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Sample records for psu ss ed

  1. The WZNW model on PSU(1, 1 vertical stroke 2)

    Energy Technology Data Exchange (ETDEWEB)

    Goetz, G. [CEA Centre d' Etudes de Saclay, 91 - Gif-sur-Yvette (France). Service de Physique Theorique; Quella, T. [King' s College London (United Kingdom). Dept. of Mathematics]|[Amsterdam Univ. (Netherlands). KdV Institute for Mathematics; Schomerus, V. [CEA Centre d' Etudes de Saclay, 91 - Gif-sur-Yvette (France). Service de Physique Theorique]|[Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)

    2006-10-15

    According to the work of Berkovits, Vafa and Witten, the non-linear sigma model on the supergroup PSU(1,1 vertical stroke 2) is the essential building block for string theory on AdS{sub 3}xS{sup 3}xT{sup 4}. Models associated with a non-vanishing value of the RR flux can be obtained through a psu(1,1 vertical stroke 2) invariant marginal deformation of the WZNW model on PSU(1,1 vertical stroke 2). We take this as a motivation to present a manifestly psu(1,1 vertical stroke 2) covariant construction of the model at the Wess-Zumino point, corresponding to a purely NSNS background 3-form flux. At this point the model possesses an enhanced psu(1,1 vertical stroke 2) current algebra symmetry whose representation theory, including explicit character formulas, is developed systematically in the first part of the paper. The space of vertex operators and a free fermion representation for their correlation functions is our main subject in the second part. Contrary to a widespread claim, bosonic and fermionic fields are necessarily coupled to each other. The interaction changes the supersymmetry transformations, with drastic consequences for the multiplets of localized normalizable states in the model. It is only this fact which allows us to decompose the full state space into multiplets of the global supersymmetry. We analyze these decompositions systematically as a preparation for a forthcoming study of the RR deformation. (orig.)

  2. Concurrent psu dic(21)(q22.3)

    Indian Academy of Sciences (India)

    Velásquez A. I. 2014 Concurrent psu dic(21)(q22.3) and t(13;17)(q14.1;p12) in a mosaic Down's syndrome patient: review of thirty-one similar dicentrics. J. Genet. 93, 189–192]. Introduction. Pseudodicentric 'mirror-image' chromosomes 21 with break-.

  3. Waffen-SS

    DEFF Research Database (Denmark)

    Poulsen, Niels Bo; Scharff Smith, Peter; Bundgård Christensen, Claus

    Bogen analyserer SS-organisationens væbnede gren, Waffen-SS ud fra en række vinkler. De vigtigste er: 1) koblingen mellem Waffen-SS og resten af SS, 2) Waffen-SS som militær faktor i Det Tredje Rige, 3) Waffen-SS soldaternes politiske indoktrinering, 4) relationerne mellem de mange nationaliteter...

  4. Draft genome sequence of Thermoanaerobacterium sp. strain PSU-2 isolated from thermophilic hydrogen producing reactor.

    Science.gov (United States)

    O-Thong, Sompong; Khongkliang, Peerawat; Mamimin, Chonticha; Singkhala, Apinya; Prasertsan, Poonsuk; Birkeland, Nils-Kåre

    2017-06-01

    Thermoanaerobacterium sp. strain PSU-2 was isolated from thermophilic hydrogen producing reactor and subjected to draft genome sequencing on 454 pyrosequencing and annotated on RAST. The draft genome sequence of strain PSU-2 contains 2,552,497 bases with an estimated G + C content of 35.2%, 2555 CDS, 8 rRNAs and 57 tRNAs. The strain had a number of genes responsible for carbohydrates metabolic, amino acids and derivatives, and protein metabolism of 17.7%, 14.39% and 9.81%, respectively. Strain PSU-2 also had gene responsible for hydrogen biosynthesis as well as the genes related to Ni-Fe hydrogenase. Comparative genomic analysis indicates strain PSU-2 shares about 94% genome sequence similarity with Thermoanaerobacterium xylanolyticum LX-11. The nucleotide sequence of this draft genome was deposited into DDBJ/ENA/GenBank under the accession MSQD00000000.

  5. The pPSU Plasmids for Generating DNA Molecular Weight Markers.

    Science.gov (United States)

    Henrici, Ryan C; Pecen, Turner J; Johnston, James L; Tan, Song

    2017-05-26

    Visualizing nucleic acids by gel electrophoresis is one of the most common techniques in molecular biology, and reference molecular weight markers or ladders are commonly used for size estimation. We have created the pPSU1 & pPSU2 pair of molecular weight marker plasmids which produce both 100 bp and 1 kb DNA ladders when digested with two common restriction enzymes. The 100 bp ladder fragments have been optimized to migrate appropriately on both agarose and native polyacrylamide, unlike many currently available DNA ladders. Sufficient plasmid DNA can be isolated from 100 ml E. coli cultures for the two plasmids to produce 100 bp or 1 kb ladders for 1000 gels. As such, the pPSU1 and pPSU2 plasmids provide reference fragments from 50 to 10000 bp at a fraction of the cost of commercial DNA ladders. The pPSU1 and pPSU2 plasmids are available without licensing restrictions to nonprofit academic users, affording freely available high-quality, low-cost molecular weight standards for molecular biology applications.

  6. Distribution of PSU Scores for Dentistry Schools in Chile during 2012-2014.

    Directory of Open Access Journals (Sweden)

    Luis Espinoza

    2014-06-01

    Full Text Available To describe distribution of applicants’ scores in the University Selection Test (Prueba de Selección Universitaria, PSU for dentistry in Chilean universities associated with the PSU test between 2012 and 2014. Methodology: A retrospective study based on the applicants’ records to dentistry in universities associated with the PSU admission process from 2012 to 2014. Results: There were twenty careers, representing sixteen universities associated with the PSU process. From them, ten were private and ten traditional. Overall, 19,469 students applied and 6,105 were summoned to fill 4,828 vacancies during the period under study. There was a drop in the number of applicants per vacancy from 5.30 to 3.11 and the selection per quota rose from 1.23 to 1.32, varying mostly in private universities. The weighted average score was 662.78 ± 44.73 with a steady decline without differences regarding sex mostly in private universities. Conclusions: During 2012-2014, there were fewer candidates for dentistry, as well as lower scores primarily affecting private schools.

  7. GyPSuM: A Detailed Tomographic Model of Mantle Density and Seismic Wave Speeds

    Energy Technology Data Exchange (ETDEWEB)

    Simmons, N A; Forte, A M; Boschi, L; Grand, S P

    2010-03-30

    GyPSuM is a tomographic model fo mantle seismic shear wave (S) speeds, compressional wave (P) speeds and detailed density anomalies that drive mantle flow. the model is developed through simultaneous inversion of seismic body wave travel times (P and S) and geodynamic observations while considering realistic mineral physics parameters linking the relative behavior of mantle properties (wave speeds and density). Geodynamic observations include the (up to degree 16) global free-air gravity field, divergence of the tectonic plates, dynamic topography of the free surface, and the flow-induced excess ellipticity of the core-mantle boundary. GyPSuM is built with the philosophy that heterogeneity that most closely resembles thermal variations is the simplest possible solution. Models of the density field from Earth's free oscillations have provided great insight into the density configuration of the mantle; but are limited to very long-wavelength solutions. Alternatively, simply scaling higher resolution seismic images to density anomalies generates density fields that do not satisfy geodynamic observations. The current study provides detailed density structures in the mantle while directly satisfying geodynamic observations through a joint seismic-geodynamic inversion process. Notable density field observations include high-density piles at the base of the superplume structures, supporting the fundamental results of past normal mode studies. However, these features are more localized and lower amplitude than past studies would suggest. When we consider all seismic anomalies in GyPSuM, we find that P and S-wave speeds are strongly correlated throughout the mantle. However, correlations between the high-velocity S zones in the deep mantle ({approx} 2000 km depth) and corresponding P-wave anomalies are very low suggesting a systematic divergence from simplified thermal effects in ancient subducted slab anomalies. Nevertheless, they argue that temperature variations are

  8. Terezine derivatives from the fungus Phoma herbarum PSU-H256.

    Science.gov (United States)

    Maha, Athip; Rukachaisirikul, Vatcharin; Saithong, Saowanit; Phongpaichit, Souwalak; Poonsuwan, Wimarak; Sakayaroj, Jariya; Saparpakorn, Patchreenart; Hannongbua, Supa

    2016-02-01

    Investigation of the fungus Phoma herbarum PSU-H256 isolated from a leaf of Hevea brasiliensis resulted in the isolation of eight terezine derivatives (E-L) together with four known compounds. Their structures were established by analysis of spectroscopic evidence. For terezines E and H, their structures were confirmed by single-crystal X-ray diffraction crystallography. In addition, the absolute configuration at C-7 in terezine E was established by Mosher's method. Terezines K and L were tested for antibacterial, antimalarial, antimycobacterial and cytotoxic activities, but were inactive. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Characterization of the various catalyst for solvent hydrogenation at 1t/d PSU; 1t/d PSU ni okeru kakushu yozai suisoka shokubai no seino hyoka

    Energy Technology Data Exchange (ETDEWEB)

    Kakebayashi, H.; Nogami, Y.; Inokuchi, K. [Mitsui SRC Development Co. Ltd., Tokyo (Japan); Aihara, Y.; Imada, K. [Nippon Steel Corp., Tokyo (Japan)

    1996-10-28

    Performance of various catalysts for hydrogenation of recycle solvent was evaluated for the operation of NEDOL process 1 t/d process supporting unit (PSU). Distillate between 220 and 538{degree}C derived from the liquefaction of Tanito Harum coal was used as recycle solvent. Deactivation behaviors of catalysts were compared using a prediction equation of catalyst life, by which aromatic carbon index (fa) after hydrogenation can be determined from the fa of recycle oil before hydrogenation, reaction temperature, and total hydrogenation time. Total hydrogenation time satisfying the {Delta}fa, 0.05 before and after hydrogenation were 8,000, 4,000, and 2,000 hours for NiMo-based catalysts C, A, and B, respectively. Catalyst C showed the longest life. Used catalysts were also characterized. The catalyst C showed larger mean pore size than those of the others, which resulted in the longer life due to the delay of pore blockage. From measurements by XPS and EPMA, relative atomic concentration of carbon increased remarkably after the use for all of catalysts, which was considered to be due to the adhesion of hydrocarbons. Increase of metal atoms, such as Fe and Cr, was also observed due to the contamination of entrainment residues. Deactivation of catalysts was caused by the adhesion of hydrocarbons, and metallic compounds, such as Fe and Cr. 3 refs., 1 fig., 5 tabs.

  10. Coal conversion rate in 1t/d PSU liquefaction reactor; 1t/d PSU ekika hannoto ni okeru sekitan tenka sokudo no kento

    Energy Technology Data Exchange (ETDEWEB)

    Ikeda, K.; Imada, K. [Nippon Steel Corp., Tokyo (Japan); Nogami, Y.; Inokuchi, K. [Mitsui SRC Development Co. Ltd., Tokyo (Japan)

    1996-10-28

    To investigate the coal liquefaction characteristics, coal slurry samples were taken from the outlets of the reactors and slurry preheater of NEDOL process 1 t/d process supporting unit (PSU), and were analyzed. Tanito Harum coal was used for liquefaction, and the slurry was prepared with recycle solvent. Liquefaction was performed using synthetic iron sulfide catalyst at reaction temperatures, 450 and 465{degree}C. Solubility of various solid samples was examined against n-hexane, toluene, and tetrahydrofuran (THF). When considering the decrease of IMO (THF-insoluble and ash) as a characteristic of coal conversion reaction, around 20% at the outlet of the slurry preheater, around 70% within the first reactor, and several percents within the successive second and third reactors were converted against supplied coal. Increase of reaction temperature led to the increase of evaporation of oil fraction, which resulted in the decrease of actual slurry flow rate and in the increase of residence time. Thus, the conversion of coal was accelerated by the synergetic effect of temperature and time. Reaction rate constant of the coal liquefaction was around 2{times}10{sup -1} [min{sup -1}], which increased slightly with increasing the reaction temperature from 450 to 465{degree}C. 3 refs., 5 figs., 1 tab.

  11. Coal liquefaction in early stage of NEDOL process 1t/d PSU; 1t/d PSU ni okeru ekika shoki hanno ni kansuru kento

    Energy Technology Data Exchange (ETDEWEB)

    Ikeda, K.; Kawabata, M.; Mochizuki, M.; Imada, K. [Nippon Steel Corp., Tokyo (Japan); Nogami, Y.; Inokuchi, K. [Mitsui SRC Development Co. Ltd., Tokyo (Japan)

    1996-10-28

    To investigate the behavior of coal liquefaction reaction in early stage as a part of studies on the coal liquefaction characteristics using NEDOL process 1 t/d process supporting unit (PSU), coal slurry sample was taken from the outlet of slurry preheater located in the upflow of liquefaction reactors, and was tested. Tanito Harum coal was used for liquefaction. Preheater was operated under the condition of pressure of 170 kg/cm{sup 2}, gas flow rate of 64 Nm{sup 3}/hr, and at temperature up to 410{degree}C at the outlet, in response to the standard test condition. The slurry sample was discharged into a high temperature separator with temperature of 250{degree}C. Liquefaction was not proceeded at the outlet of preheater. Solid residue yielded around 80%, and liquid yielded around 15%. Gases, CO and CO2, and water yielded also small amount around 3%. The solid sample contained much IOM fraction (tetrahydrofuran-insoluble and ash), and the liquid contained much heavy oil fraction. Hydrogenation was not proceeded, and the hydrogen consumption was very low showing below one-tenth of that at the usual operation. Hydrogen sulfide gas was formed at early stage, which suggested that the change of iron sulfide catalyst occur at early stage of liquefaction. 1 ref., 5 figs., 2 tabs.

  12. Fiscal 1997 achievement report. Coal liquefaction technology development - Bituminous coal liquefaction technology development - Study for supporting pilot plant - Study using 1t/d PSU (Study of operation using PSU); 1997 nendo seika hokokusho. Sekitan ekika gijutsu kaihatsu - Rekiseitan ekika gijutsu no kaihatsu - Pilot plant no shien kenkyu - 1t/d process support unit (PSU) ni yoru kenkyu (PSU ni yoru unten kenkyu)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-03-01

    The operation of a 1t/d PSU (process support unit) was studied for the NEDOL coal liquefaction process. In the modification of facilities, equipment and devices were procured for the product oil reforming facilities and their installation was partly accomplished. In the coal injection operation, a 60-day slurry operation was conducted, using coal types of the upper limit and lower limit qualities, for expanding the scope of coal types applicable to the NEDOL process and for exploring optimum conditions, and another operation of 37 days was conducted using Chinese coal and Chinese-prepared liquefaction catalysts, and the two operations were studied for difference in yields and in operationality. Characteristics of the liquefaction reactors were investigated and basic studies were made relative to the physical property of the yielded coal oil. In the operation for maintenance, the 1st liquefaction reactor was singly operated for an 8-day slurry operation, which was to check the progress of liquefaction in a 1-reactor setup. Concerning the reforming of the product oil, the hydrogenation reactors were checked for their response to temperature control. Moreover, hydrogenation solvents were produced for the PSU and for China. (NEDO)

  13. CARTOGRAPHIE DU PLASMIDE pSU100, PLASMIDE CRYPTIQUE DE LACTOBACILLUS CASEI

    Directory of Open Access Journals (Sweden)

    F BENSALAH

    2003-06-01

    Ce plasmide appelé pSU100 a été cloné dans le vecteur de transformation pUC18 au site EcoRI chez E. coli JM103. Les profils électrophorétiques de restriction obtenus par des digestions simples, doubles et triples sous l’action de 33 endonucléases, ont contribué à l’élaboration d’une carte de restriction de ce plasmide. Cinq sites uniques ont été identifiés, ainsi que d’autres sites doubles et multiples. Une étude préliminaire du rôle physiologique de ce plasmide a permis de déceler une résistance à la kanamycine.

  14. High-rate continuous hydrogen production by Thermoanaerobacterium thermosaccharolyticum PSU-2 immobilized on heat-pretreated methanogenic granules

    DEFF Research Database (Denmark)

    O-Thong, Sompong; Prasertsan, P.; Karakashev, Dimitar Borisov

    2008-01-01

    Biohydrogen production from Thermoanaerobacterium thermosaccharolyticum strain PSU-2 was examined in upflow anaerobic sludge blanket (UASB) reactor and carrier-free upflow anaerobic reactor (UA), both fed with sucrose and operating at 60 degrees C. Heat-pretreated methanogenic granules were used...

  15. Fiscal 1998 achievement report. Coal liquefaction technology development - Bituminous coal liquefaction technology development - Study for supporting pilot plant - Study using 1t/d PSU (Study of operation using PSU); 1998 nendo seika hokokusho. Sekitan ekika gijutsu kaihatsu - Rekiseitan ekika gijutsu no kaihatsu - Pilot plant no shien kenkyu - 1t/d process support unit (PSU) ni yoru kenkyu (PSU ni yoru unten kenkyu)

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1999-06-01

    The operation of a 1t/d PSU (process support unit) was studied for the NEDOL coal liquefaction process. For the modification of facilities, product oil reforming facilities were installed. In the coal injection operation, a 59-day slurry operation was carried out using Tanito Harum coal, which was for the evaluation of the reforming effect under the product reforming conditions, operationality, and liquefaction characteristics. A 37-day slurry operation was also performed using Chinese coal, which was intended to pursue improved cost performance and to collect the light oil fraction in the flow of reforming operation. In the operation for maintenance, investigations were conducted into conditions for hydrogenation in a 1-through operation involving the solvent hydrogenation process and the normal pressure distillation facilities and into the effect of pre-hydrogenation solvent properties, which aimed at grasping the effect of product oil reforming measures and their influence on operationality. In the survey of environments, the properties of wastewater from the liquefaction process using Tanito Harum coal were studied and toxicity evaluation tests were conducted for activated sludge. (NEDO)

  16. Site description of the SFR area at Forsmark at completion of the site investigation phase. SDM-PSU Forsmark

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2013-05-15

    The site descriptive model (SDM) presented in this report is an integrated model for bedrock geology, rock mechanics, bedrock hydrogeology and bedrock hydrogeochemistry of the site investigated in the SFR extension project (PSU). A description of the surface system is also included in the report. However, the surface system is not integrated with the other disciplines as new data regarding the surface system will not be available until after the completion of SDM-PSU. It is noted that SDM-PSU does not include all disciplines handled in SDM-Site Forsmark (SKB 2008b), the focus is to produce a site description that meets the needs of the SFR extension project. The overall objective of the SFR extension project is to have the application for the extension ready by 2013. This report presents an integrated site model incorporating the historic data acquired from the investigations for and construction of the existing SFR facility (1980-1986), as well as from the recent investigations for the planned extension of SFR (2008-2009). It also provides a summary of the abundant underlying data and the discipline-specific models that support the integrated site model. The description relies heavily on background reports concerning detailed data analyses and modelling in the different disciplines. It is noteworthy that the investigations conducted during the SFR extension project were guided by the choice of site prior to the investigations, which was based on the experience gained during the construction of the existing SFR facility.

  17. Inform@ed space

    DEFF Research Database (Denmark)

    Bjerrum, Peter; Olsen, Kasper Nefer

    2001-01-01

    Inform@ed space Sensorial Perception And Computer Enchancement - bidrag til Nordisk Arkitekturforskningsforenings IT-konference, AAA april 2001.......Inform@ed space Sensorial Perception And Computer Enchancement - bidrag til Nordisk Arkitekturforskningsforenings IT-konference, AAA april 2001....

  18. Sample Preparation (SS): SE59_SS01 [Metabolonote[Archive

    Lifescience Database Archive (English)

    Full Text Available SE59_SS01 Drought stress Three-week-old plants vertically grown on GM plates were i...ncubated in 24-well plates (TPP, http://www.tpp.ch/index.php). Each plant was put in a well with 3 ml water. Three

  19. PSU/WES Interlaboratory Comparative Methodology Study of an Experimental Cementitious Repository Seal Material. Report 2. Final Results.

    Science.gov (United States)

    1982-03-01

    1979. High-range water-reducing admixture ("superplasticizer"), naphthalene-sUlfonate condensate. (f) Defoaming Agent (PSU #A27) - Received May 1979. (g...naphthalene-sulfonate condensate. (e) WES AD-599, a defoamer , received 2 April 1979. (f) Mixing water, deionized, boiled, and stored, in closed...Solid Expansive Additive A31 8.34 Material NaCl B08 4.05 Water Reducer A28 1.099 Premixed Defoaming Agent A27 0.02 Solution Freshly boiled dionized

  20. SS

    African Journals Online (AJOL)

    1814,written by certain Lieutenant-Colonel William. Fuller of the King's Dragoon Guards to a British. Lord, in which ... The colonel was purely an administrative appoint- ment made by the government, and he was given a. .... motion to mark some notable event such as the successful conclusion of hostilities or the accession.

  1. Molecular characterization, prevalence and clinical relevance of Phodopus sungorus papillomavirus type 1 (PsuPV1) naturally infecting Siberian hamsters (Phodopus sungorus).

    Science.gov (United States)

    Kocjan, Boštjan J; Hošnjak, Lea; Račnik, Joško; Zadravec, Marko; Bakovnik, Nejc; Cigler, Blaž; Ummelen, Monique; Hopman, Anton H N; Gale, Nina; Švara, Tanja; Gombač, Mitja; Poljak, Mario

    2017-11-01

    Phodopus sungorus papillomavirus type 1 (PsuPV1), naturally infecting Siberian hamsters (Phodopus sungorus) and clustering in the genus Pipapillomavirus (Pi-PV), is only the second PV type isolated from the subfamily of hamsters. In silico analysis of three independent complete viral genomes obtained from cervical adenocarcinoma, oral squamous cell carcinoma and normal oral mucosa revealed that PsuPV1 encodes characteristic viral proteins (E1, E2, E4, E6, E7, L1 and L2) with conserved functional domains and a highly conserved non-coding region. The overall high prevalence (102/114; 89.5 %) of PsuPV1 infection in normal oral and anogenital mucosa suggests that asymptomatic infection with PsuPV1 is very frequent in healthy Siberian hamsters from an early age onward, and that the virus is often transmitted between both anatomical sites. Using type-specific real-time PCR and chromogenic in situ hybridization, the presence of PsuPV1 was additionally detected in several investigated tumours (cervical adenocarcinoma, cervical adenomyoma, vaginal carcinoma in situ, ovarian granulosa cell tumour, mammary ductal carcinoma, oral fibrosarcoma, hibernoma and squamous cell papilloma) and normal tissues of adult animals. In the tissue sample of the oral squamous cell carcinoma individual, punctuated PsuPV1-specific in situ hybridization spots were detected within the nuclei of infected animal cells, suggesting viral integration into the host genome and a potential etiological association of PsuPV1 with sporadic cases of this neoplasm.

  2. Line locking and SS 433

    Science.gov (United States)

    Pekarevich, M.; Piran, T.; Shaham, J.

    1984-01-01

    The general problem of acceleration by line locking in an optically thin medium is considered. Analytic solutions to the coupled equations of radiation transfer and matter motion are presented. Using these solutions, restrictions on the physical conditions of the accelerated matter are derived. By applying these conditions to SS 433, it is found that if the absorbing ion is light (hydrogen or helium), the matter must be highly clumped, and the acceleration has to begin at approximately 10 to the 12th cm. Line-locking acceleration with a hydrogen-like heavy ion such as iron demands higher than solar ion abundance.

  3. PREFACE: EDS2010 Preface

    Science.gov (United States)

    Heggie, Malcolm I.

    2011-03-01

    The biennial international conference on Extended Defects in Semiconductors started in 1978 with a meeting in Hünfeld, Germany. Subsequent meetings rotated between Poland, France, Great Britain, Germany, Russia and Italy, culminating in EDS2004 in Chernogolovka, EDS2006 in Halle and EDS2008 in Poitiers. EDS2010 was held at the University of Sussex at Brighton, UK from September 19th to 24th. An extension of the tabulation of this history, which first appeared on the EDS2006 website, is given in the attached PDF. It is with sadness that we note one of the founders of the series, Prof. Dr Helmut Alexander, passed away on 3 December 2009 and we were proud to dedicate EDS2010 to his memory. It has become a tradition to make an award in his name, and this year it was made to Ivan Isacov for his poster "Electrical levels of dislocation networks in p- and n-type silicon". A short and warm celebration of Prof. Dr Alexander's life by his friends and colleagues, Prof. Drs Helmut Gottschalk, Eicke Weber and Wolfgang Schröter, is included in this volume. The conference was a forum for the state-of-the-art of investigation and modelling of extended defects in semiconductors. Scientists from universities, research institutes and industry made contributions to a deeper understanding of extended defects, their interaction with point defects and their role in the development of semiconductor technology. The remit of the conference included extended defects, nanostructures, nanoparticles, quantum dots and interfaces within semiconducting materials ranging from narrow to wide band gaps, including graphene-derived materials and diamond. Scientific interests range from defect geometry, electronic structure, dynamics, spectroscopy, microscopy, reactions and chemistry to introduction mechanisms, such as implantation and strained layers and the operation of devices such as integrated circuits, heterostructures, and solar cells. The organisers were confronted with a long period between

  4. Efficient production of polyhydroxyalkanoates (PHAs) from Pseudomonas mendocina PSU using a biodiesel liquid waste (BLW) as the sole carbon source.

    Science.gov (United States)

    Chanasit, Wankuson; Hodgson, Brian; Sudesh, Kumar; Umsakul, Kamontam

    2016-07-01

    Conditions for the optimal production of polyhydroxyalkanoate (PHA) by Pseudomonas mendocina PSU using a biodiesel liquid waste (BLW) were determined by response surface methodology. These were an initial carbon to nitrogen ratio (C/N) of 40 (mole/mole), an initial pH of 7.0, and a temperature of 35 °C. A biomass and PHA concentration of 3.65 g/L and about 2.6 g/L (77% DCW), respectively, were achieved in a growth associated process using 20 g/L glycerol in the BLW after 36 h of exponential growth. The PHA monomer compositions were 3HB (3-hydroxybutyrate), a short-chain-length-PHA, and the medium-chain-length-PHA e.g. 3-hydroxyoctanoate and 3-hydroxydecanoate. Both the phbC and phaC genes were characterized. The phbC enzyme had not been previously detected in a Pseudomonas mendocina species. A 2.15 g/L of an exopolysaccharide, alginate, was also produced with a similar composition to that of other Pseudomonas species.

  5. Työhyvinvointi ja työssä jaksaminen hoitotyössä

    OpenAIRE

    Marjomaa, Tiina; Niemonen, Soile

    2013-01-01

    Työhyvinvointiin ja työssä jaksamiseen liittyvät tekijät ovat erittäin paljon esillä sairaanhoitajan työssä. Työhyvinvointi on menestymisen pohja jokaiselle työntekijälle ja työyhteisölle. Tässä työssä vastataan tutkimustehtäviin, miten fyysinen, psyykkinen ja sosiaalinen hyvinvointi näkyvät työssä, mikä on johtamisen merkitys hyvinvoinnille ja minkälainen merkitys on sukupolvisuudella työssäviihtymiselle. Haemme opinnäytetyössämme vastauksia siihen, mitkä tekijät edistävät tai estävät sa...

  6. Magnitude analysis of aftershocks of the Mw 9.3 Sumatra-Andaman Earthquake recorded at the temporary PSU Seismic Station in Phang nga, Thailand

    Directory of Open Access Journals (Sweden)

    Helmut Düerrast

    2008-03-01

    Full Text Available After the Mw 9.3 Sumatra-Andaman Earthquake on 26 December 2004, a temporary broadband seismic station was set up at the Khao Chang Telecommunication Station in Phang nga Province, Southern Thailand, in order to monitor aftershocks in an area bounded by 0o-20o N, and 90o-100o E. Altogether 98 events were identified during the study period from 1st to 12th January 2005; but six of these events are not listed in the catalog of the United States Geological Survey (USGS/ NEIC. Body wave magnitudes (mb and moment magnitudes (Mw of the events were determined and compared with USGS magnitudes. For mb, the PSU values are 0.215 higher than the USGS values, whereas for Mw, the PSU values are 0.268 lower than the USGS values. Differences in mb may result from differences in time windows chosen for the maximum amplitude determination, or from accuracy of the Q-value. Differences in Mw may result from assumptions made on velocity, attenuation and determination of the low-frequency part of the seismic spectrum. However, these differences are comparatively small considering that the USGS data are network values whereas the PSU data are from a single station.

  7. Molecular cloning, characterization and differential expression of a Sporothrix schenckii STE20-like protein kinase SsSte20.

    Science.gov (United States)

    Zhang, Zhenying; Hou, Binbin; Zheng, Fangliang; Yu, Xiaohong; Liu, Xiaoming

    2013-06-01

    Dimorphic switching requires fungal cells to undergo changes in polarized growth in response to environmental stimuli. The Ste20-related kinases are involved in signaling through mitogen-activated protein kinase pathways and in morphogenesis through the regulation of cytokinesis and actin-dependent polarized growth. In this report, we isolated and characterized an Ste20 homologue gene, designated SsSte20, from yeast-form Sporothrix schenckii (S. schenckii). The full length SsSte20 cDNA sequence is 2846 bp in size, and contains an open reading frame of 2505 bp encoding 835 amino acids. The predicted molecular mass of SsSte20 is 91.31 kDa with an estimated theoretical isoelectric point of 5.76. SsSte20 kinase domain shows 63% identity with that of Don3, a germinal centre kinase (GCK) from Ustilago maydis. Two exons and one intron are identified within the 2578 bp SsSte20 genomic DNA sequence of S. schenckii. Differential expression of the SsSte20 was demonstrated by real-time RT-PCR. The expression of SsSte20 was much higher in the yeast stage compared with that in the mycelial stage, which indicated that the SsSte20 may be involved in the pathogenesis of the yeast phase of S. schenckii.

  8. EDS becomes CERN Openlab contributor

    CERN Multimedia

    2007-01-01

    "EDS today announced that it has become an official contributor to CERN openlab. The purpose of the joint project between CERN and EDS is to carry out research and development in the field of monitoring, management and operaiton of grid services." (2/3 page)

  9. Rebooting the EdD

    Science.gov (United States)

    Wergin, Jon F.

    2011-01-01

    In this essay, Jon Wergin reminds readers of the philosophical and historical foundations of the doctor of education (EdD) degree. He argues that the EdD should be based, in large part, on John Dewey's progressive ideals of democratization and Paulo Freire's concepts of emancipatory education. Drawing on theories of reflective practice,…

  10. EDS becoms CERN Openlab contributor

    CERN Multimedia

    2007-01-01

    "EDS announced that it has become an official contributor to CERN openlab. The purpose of the joint project beteween CERN and EDS is to carry out research and development in the field of monitoring, management and operation of grid services." (1 page)

  11. Muutosjohtaminen Lindorff Oy.ssä

    OpenAIRE

    Huuskonen, Ville

    2011-01-01

    Muutos on pysyvä tila nykyajan liiketoiminnassa. Kehittynyt teknologia, vaativammat asiakkaat ja kovempi kilpailu ovat tekijöitä, joiden mukaan yritysten tulee pystyä kehittämään toimintaansa. Yritysten toimintaympäristö muuttuu jatkuvasti ja paikallaan pysyvät stabiilit yritykset eivät enää menesty kilpailussa. Muutos tuo mahdollisuuksien lisäksi myös epävarmuutta. Onnistunut muutosprosessi vaatii aina hyvää johtamista. Tässä opinnäytetyössä tarkastellaan muutosjohtamisen haasteita...

  12. SS-penogram: a new diagnostic test for erectile dysfunction.

    Science.gov (United States)

    Choi, Hyung Ki; Choi, Yeong Jin; Choi, Young Deuk; Rha, Koon Ho; Kim, Jang Hwan; Kim, Dong Kee

    2002-02-01

    The clinical reports on Sildenafil sulfate (Viagra) are mainly based on individual observations. However, there is a paucity of objective studies in the literature. In order to objectively examine the effect of Sildenafil, a SS (Sexual Stimulation)-Penogram that is a non-invasive, simple and physiologic method was developed using a radioisotope (RI). One hundred and four SS-penograms were performed on patients who had a documented erectile dysfunction (ED) lasting for more than 6 months. After an intravenous injection of 99mTc-RBC (15 mCi), the first penogram was taken immediately after sexual stimulation, which was done by 30 minutes of erotic videotape viewing. Forty minutes after administering 25 to 100 mg of Sildenafil, a second penogram was taken. The characteristics of each penogram were analyzed according to a previously reported method. The results were graded as follows; Type I(normal function; 5 min or more of peak erectile response with an induction period of 1 to 6 min), Type II-A (impossible function type; i.e., showing less than 2 times the basal radioactivity level), Type II-B (the unstable type; showing less than 5 min of peak erectile response), and Type II-C (the delayed type; which showed a delay of more than 15 min after the start of sexual stimulation). The patients were grouped according to their response after Sildenafil administration, and the effect of Sildenafil was assessed by comparing the radioactivity from between 7 to 22 minutes and the changes in the characteristics of the penogram. The mean age of the patients was 44.9 +/- 10.2 (23 - 68) years. In the first penogram, Type I was found in 12 patients, and Type II-A in 14, Type II-B in 73, Type II-C in 1 and a mixed (II-B + C) type was found in 4 patients. A second penogram after Sildenafil administration, showed Type I in 46 patients, and Type II-A in 10, Type II-B in 46 and a mixed type was found in 2 patients. The responses after Sildenafil were categorized as follows: 1) An

  13. TechEdSat Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The overall goal of the Technical Education Satellite (TechEdSat) is to employ a small spacecraft to evaluate, demonstrate, and validate two new technologies for...

  14. Sample Preparation (SS) - Metabolonote | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available switchLanguage; BLAST Search Image Search Home About Archive Update History Data List Contact us Metabol...ed to by S (Sample information). Data file File name: metabolonote_sample_prepara...tion_details.zip File URL: ftp://ftp.biosciencedbc.jp/archive/metabolonote/LATEST/metabolonote_sample_prepar...ation_details.zip File size: 13 KB Simple search URL http://togodb.biosciencedbc.jp/togodb/view/metabolonote...ption Download License Update History of This Database Site Policy | Contact Us Sample Preparation (SS) - Metabolonote | LSDB Archive ...

  15. Investigation of the deposit formation in pipelines connecting liquefaction reactors; 1t/d PSU ni okeru ekika hanno tokan fuchakubutsu no seisei yoin ni kansuru ichikosatsu

    Energy Technology Data Exchange (ETDEWEB)

    Okada, Y.; Nogami, Y.; Inokuchi, K. [Mitsui SRC Development Co. Ltd., Tokyo (Japan); Mochizuki, M.; Imada, K. [Nippon Steel Corp., Tokyo (Japan)

    1996-10-28

    The liquefaction reaction system of an NEDOL process coal liquefaction 1t/d PSU was opened and checked to investigate the cause of the rise of differential pressure between liquefaction reactors of the PSU. The liquefaction test at a coal concentration of 50 wt% using Tanito Harum coal was conducted, and it was found that the differential pressure between reactors was on the increase. By the two-phase flow pressure loss method, deposition thickness of deposit in pipelines was estimated at 4.4mm at the time of end operation, which agreed with a measuring value obtained from a {gamma} ray. The rise of differential pressure was caused by deposit formation in pipelines connecting reactors. The main component of the deposit is calcite (CaCO3 60-70%) and is the same as the usual one. It is also the same type as the deposit on the reactor wall. Ca in coal ash is concerned with this. To withdraw solid matters deposited in the reactor, there are installed pipelines for the withdrawal at the reactor bottom. The solid matters are regularly purged by reverse gas for prevention of clogging. As the frequency of purge increases, the deposit at the reactor bottom decreases, but the deposit attaches strongly to pipelines connecting reactors. It is presumed that this deposit is what Ca to be discharged out of the system as a form of deposition solid matter naturally in the Ca balance precipitated as calcite in the pipeline connecting the reactor. 3 refs., 5 figs., 4 tabs.

  16. Democrazia ed élites

    Directory of Open Access Journals (Sweden)

    N. BOBBIO

    2014-07-01

    Full Text Available L’articolo si propone di mostrare che la teoria della classe politica, formulata ed elaborata da scrittori conservatori con precisi intenti di polemica antidemocratica, è stata utilizzata anche da scrittori decoratici; in secondo luogo di confermare la tesi cara a Pareto, secondo cui altro è il valore scientifico di una teoria politica, altro il suo uso ideologico

  17. Mitigating Higher Ed Cyber Attacks

    Science.gov (United States)

    Rogers, Gary; Ashford, Tina

    2015-01-01

    In this presentation we will discuss the many and varied cyber attacks that have recently occurred in the higher ed community. We will discuss the perpetrators, the victims, the impact and how these institutions have evolved to meet this threat. Mitigation techniques and defense strategies will be covered as will a discussion of effective security…

  18. 35 years of EDS software.

    Science.gov (United States)

    Schamber, Frederick H

    2009-12-01

    The computerized multichannel analyzer running software specifically designed for X-ray analysis appeared very early in the commercialization of the energy dispersive X-ray spectrometer (EDS) and, like the solid-state X-ray detector itself, was built on a technology foundation originally developed for nuclear spectroscopy. However, software techniques employed for gamma-ray spectra could not accommodate the continuum component of EDS spectra, and a new approach was required. Least-squares fitting with "top-hat" filtered spectra proved to be an effective solution that is still widely used today. Though modern computer technology has subsequently contributed greatly to the speed and convenience of present-day EDS software, it seems that the achievable accuracy and precision of spectrum analysis has not fundamentally improved, and most of the early challenges are still quite relevant, although they may appear in new guises. The availability of the high speed silicon drift detector, however, may provide both the incentive and the data precision to drive future advances. This article traces the formative years of EDS software from the personalized perspective of a participant. Factors that shaped the development of the industry are identified, and future directions are speculated.

  19. Ed Watson 1940-2006

    CERN Document Server

    2006-01-01

    Ed Watson arrived at CERN in March 1973 to work on digital electronics and CAMAC systems under Bob Dobinson, after many years at Rolls Royce in Scotland. He joined the European Muon Collaboration in 1976, where he played a major role in the design, deployment and running of its data acquisition system (DAQ) with David Botterill, Bob Dobinson, and Vicky White. The CAMAC-ROMULUS system was by far the largest and most advanced of its time, and it became a defining standard for DAQ systems for years to come. Ed was deeply involved in the detailed planning of the control rooms and the experiment cabling, as well as sharing the responsibility for the CAMAC readout system. He had a real talent for trouble shooting and played a vital part in supporting the experiment throughout its lifetime. He offered great moral support to the younger members of the collaboration and helped them a great deal with their work. The EMC had a wonderful social life to which Ed was a major contributor - who can forget its barbecues?  In...

  20. Ed Watson - 1940-2006

    CERN Multimedia

    2006-01-01

    Ed Watson passed away suddenly on 1 August in Geneva, he was 66. He leaves his wife and two children. Ed Watson arrived at CERN in March 1973 to work on digital electronics and CAMAC systems under Bob Dobinson, after many years at Rolls Royce in Scotland. He joined the European Muon Collaboration in 1976, where he played a major role in the design, deployment and running of its data acquisition system (DAQ) with David Botterill, Bob Dobinson, and Vicky White. The CAMAC-ROMULUS system was by far the largest and most advanced of its time, and it became a defining standard for DAQ systems for years to come. Ed was deeply involved in the detailed planning of the control rooms and the experiment cabling, as well as sharing the responsibility for the CAMAC readout system. He had a real talent for trouble shooting and played a vital part in supporting the experiment throughout its lifetime. He offered great moral support to the younger members of the collaboration and helped them a great deal with their work. The...

  1. Molecular dynamics simulations of a single stranded (ss) DNA

    CERN Document Server

    Chatterjee, Subhasish; Thakur, Siddarth; Burin, Alexander

    2012-01-01

    The objective of the present study was to develop an understanding of short single-stranded DNA (ssDNA) to assist the development of new DNA-based biosensors. A ssDNA model containing twelve bases was constructed from the 130-145 codon sequence of the p53 gene. Various thermodynamic macroscopic observables such as temperature, energy distributions, as well as root mean square deviation (RMSD) of the nucleic acid backbone of the ssDNA were studied using molecular dynamics (MD) simulations. The AMBER program was used for building the structural model of the ssDNA, and atomistic MD simulations in three different ensembles were carried out using the NAMD program. The microcanonical (NVE), conical (NVT) and isobaric-isothermal (NPT) ensembles were employed to compare the equilibrium characteristics of ssDNA in aqueous solutions. Our results indicate that the conformational stability of the ssDNA is dependent on the thermodynamic conditions.

  2. 78 FR 55684 - ConnectED Workshop

    Science.gov (United States)

    2013-09-11

    ... National Telecommunications and Information Administration ConnectED Workshop AGENCY: National... in the United States to next- generation broadband. This Notice announces that the ConnectED Workshop... ConnectED Workshop will discuss the growing bandwidth needs of K-12 schools as more schools use mobile...

  3. AdvancED Flex 4

    CERN Document Server

    Tiwari, Shashank; Schulze, Charlie

    2010-01-01

    AdvancED Flex 4 makes advanced Flex 4 concepts and techniques easy. Ajax, RIA, Web 2.0, mashups, mobile applications, the most sophisticated web tools, and the coolest interactive web applications are all covered with practical, visually oriented recipes. * Completely updated for the new tools in Flex 4* Demonstrates how to use Flex 4 to create robust and scalable enterprise-grade Rich Internet Applications.* Teaches you to build high-performance web applications with interactivity that really engages your users.* What you'll learn Practiced beginners and intermediate users of Flex, especially

  4. Sleeping sickness (ss) in the Abraka Belt: a preliminary ...

    African Journals Online (AJOL)

    Sleeping sickness (SS) in the Abraka belt: a preliminary geographical description of the disease focus.Annals Biomedical Sciences 2002;1:111-117.This retrospective study provides an analysis of the epidemiological data on Sleeping Sickness (SS) cases between January 1999 and December 2001 at the Baptist Medical ...

  5. Two New Sharp Ostrowski-Grüss Type Inequalities

    Directory of Open Access Journals (Sweden)

    Zheng Liu

    2013-11-01

    Full Text Available The purpose of this paper is to use a variant of the Grüss inequality to derive two new sharp Ostrowski-Grüss type inequalities related to a perturbed trapezoidal type rule and a perturbed generalized interior point rule, respectively, which provide improvements of some previous results in the literatures.

  6. Molecular systematics and historical biogeography of Maianthemum s.s.

    Science.gov (United States)

    Kim, Changkyun; Cameron, Kenneth M; Kim, Joo-Hwan

    2017-06-01

    Understanding the underlying mechanisms for the evolution of intercontinental disjunct patterns has long fascinated botanists. We present a molecular phylogenetic reconstruction of Maianthemum s.l. (including Smilacina) with a focus on Maianthemum s.s. species, which have a disjunct distribution between Eurasia and North America. Within this evolutionary framework, we clarify the systematic classification and biogeographical history of Maianthemum s.s. Data from two nuclear and five chloroplast DNA regions were analyzed using the programs PAUP*, RAxML, MrBayes, BEAST, and RASP. Our molecular phylogeny supports Maianthemum s.s. as monophyletic. Maianthemum bifolium and M. canadense are grouped according to their taxonomic species, but the accessions of M. dilatatum are divided into two well-defined groups, i.e., one comprising western North American accessions, and the other composed of northeast Asian accessions. Molecular dating and biogeographic reconstructions suggest a northeast Asian origin for Maianthemum s.s. and that a complicated pattern of divergent evolution began approximately in the late Miocene. Intercontinental disjunctions of Maianthemum s.s. in the Northern Hemisphere appear to have occurred two times during the Pliocene. Based on our results, two possible ways to treat Maianthemum s.s. species can be envisioned: (1) elevate Asian populations of M. dilatatum to specific rank; or (2) combine the three Maianthemum s.s. species into a broadly defined single species. We recommend treatment of Maianthemum s.s. as a single species. The biogeographic patterns of Maianthemum s.s. coupled with molecular dating suggest both vicariance and long-distance dispersal events as key mechanisms for diversification of the clade. © 2017 Botanical Society of America.

  7. Sosiaalisen median rooli kunnan viestinnässä

    OpenAIRE

    Selkämaa, Kati

    2016-01-01

    Opinnäytetyön tavoitteena oli selvittää sosiaalisen median roolia kunnan viestinnässä sekä tutkia, miten sosiaalista mediaa hyödynnetään kuntien viestinnässä. Teoriaosuudessa tarkasteltiin sosiaalista mediaa, tutustuttiin sen tunnetuimpiin sovelluksiin sekä perehdyttiin kuntien viestintään yleisesti. Työssä tarkasteltiin myös kuntien viestintään vaikuttavia ja sitä sääteleviä lakeja. Kuntien sosiaalisen median käyttöön tutustuttiin Kuntaliiton tekemän viestintätutkimuksen tulosten pohjalt...

  8. Markkinointisuunnitelma Oy Runoilijan tie s/s Tarjanteelle

    OpenAIRE

    Suojala, Jaana

    2012-01-01

    Tämän opinnäytetyön tehtävä on markkinointisuunnitelman laatiminen s/s Tarjanteelle ja Runoilijan tie Oy:lle. Tarve työlle tuli kun toimin purjehduskauden 2011 ravintola-vastaavana laivassa. Kesän aikana kävi ilmi selkeitä puutteita varsinkin markkinoinnissa. Näihin puutteisiin on tässä opinnäytetyössä etsitty ratkaisuja teoreettisista teoksista sekä suorittamalla asiakaskysely e-lomakkeella. Lomakkeella tiedusteltiin useita eri asioita markkinoinnista, mainonnasta sekä myös palvelusta yleens...

  9. The KiSS1/GPR54 system in fish.

    Science.gov (United States)

    Elizur, Abigail

    2009-01-01

    The KiSS1/GPR54 system has now been identified in non-mammalian vertebrates. Transcripts encoding for KiSS1 and its receptor, GPR54, have been isolated from a number of fish species. The expression of their genes was characterized in the context of temporal and spatial expression and in response to endocrine manipulations. GPR54 sequence is conserved between mammals and fish, with a second receptor sequence identified in zebrafish. The KiSS1 gene sequence is highly divergent between mammals and fish, yet the human kisspeptin is capable of activating the fish GPR54. As in mammals, the fish KiSS1/GPR54 system appears to be partially regulated by gonadal steroids. The data available for fish are fragmented, yet indicate that the KiSS1/GPR54 system is functionally conserved in non-mammalian vertebrates and supports the notion that it has a role in pubertal development and reproduction in piscine systems.

  10. CellSs: Scheduling Techniques to Better Exploit Memory Hierarchy

    Directory of Open Access Journals (Sweden)

    Pieter Bellens

    2009-01-01

    Full Text Available Cell Superscalar's (CellSs main goal is to provide a simple, flexible and easy programming approach for the Cell Broadband Engine (Cell/B.E. that automatically exploits the inherent concurrency of the applications at a task level. The CellSs environment is based on a source-to-source compiler that translates annotated C or Fortran code and a runtime library tailored for the Cell/B.E. that takes care of the concurrent execution of the application. The first efforts for task scheduling in CellSs derived from very simple heuristics. This paper presents new scheduling techniques that have been developed for CellSs for the purpose of improving an application's performance. Additionally, the design of a new scheduling algorithm is detailed and the algorithm evaluated. The CellSs scheduler takes an extension of the memory hierarchy for Cell/B.E. into account, with a cache memory shared between the SPEs. All new scheduling practices have been evaluated showing better behavior of our system.

  11. Optimisation of hot isostatic pressing bonded SS/SS joints conditions for ITER blanket shield

    Energy Technology Data Exchange (ETDEWEB)

    Cedat, D. [AREVA NP Technical Centre 30, Bld de l' industrie, 71205 Le Creusot Cedex (France); Bobin, I., E-mail: isabelle.bobinvastra@areva.com [AREVA NP Technical Centre 30, Bld de l' industrie, 71205 Le Creusot Cedex (France); Boireau, B. [AREVA NP Technical Centre 30, Bld de l' industrie, 71205 Le Creusot Cedex (France); Bucci, P. [CEA DRT/Liten/DTH, 38054 Grenoble (France); Lorenzetto, P. [FUSION FOR ENERGY, Torres Diagonal Litoral, B3, Carrer Josep Pla, 2, 08019 Barcelona (Spain)

    2012-08-15

    In the engineering design activity of international thermonuclear experimental reactor (ITER), stainless steels are being considered as candidates materials for several module type structures. Hot isostatic pressing (HIP) technique is expected for the fabrication of these modules. Stainless steel powders are simultaneously consolidated as mono-material block or/and joined in bi-material module. This paper reviews the manufacturing stages, non-destructive examination and the developments of the HIP bonded joints of 316L SS (powder and solid) for application to the ITER shield blanket. It is well known that the powder surface oxidation negatively influences the impact toughness of raw material and joints consolidated by this way. In order to get acceptable mechanical properties of materials, a study on the effect of reducing the powder oxygen content has been launched. To evaluate susceptibility to the oxygen content of HIPed joint specimens, tensile and toughness tests have been performed. From this study, optimal conditions of HIP were fitted and the influence of oxygen was mastered to obtain good mechanical properties of the consolidated powder material as well as for HIPed junction.

  12. The spotted contact binary SS ARIETIS - Spectroscopy and infrared photometry

    Science.gov (United States)

    Rainger, P. P.; Bell, S. A.; Hilditch, R. W.

    1992-02-01

    The first infrared photometry for the W-UMa system SS Ari is presented. An analysis based on medium-resolution spectroscopy presented here shows that SS Ari is a W-type system with a mass ratio of 0.33. It seems certain that the asymmetry in the published light curves and those obtained for this study can be explained by the effect of spots on one or possibly both components of the system. The precise location, size and temperature of these spots require the use of Doppler Imaging techniques in conjunction with high-quality multiband photometry.

  13. Kuntosalikerho työkaluna nuorisotyössä

    OpenAIRE

    Östman, Sebastian

    2015-01-01

    Opinnäytetyöni tarkoitus oli selvittää kuinka kuntosali ja siihen liittyvä kerhotoiminta soveltuu menetelmäksi nuorisotyössä. Tutkimuksessani korostuu nuorten oma kokemus nuorisotilalla olevan kuntosalin vaikutuksista arkeen. Tutkimukseni lähestymistapana käytin toimintatutkimusta, osallistumalla ja olemalla itse mukana tutkimusympäristössä. Tutkimusympäristönä toimi Espoon Soukan ruotsinkielinen nuorisotila, Sökö ungdomslokal. Toiminta sai alkunsa vuodenvaihteessa 2013–2014, jolloin aloitin ...

  14. A new intense radio flare of SS433

    Science.gov (United States)

    Trushkin, S. A.; Nizhelskij, N. N.

    2008-10-01

    The supercritical accretor, high-mass X-ray binary, SS433 is in the very intense radio flare just in the first day of new monitoring set with the RATAN-600 radio telescope (SAO RAS). 28 October 2008 (MJD 54767.58) the measurements at 1-22 GHz show the power law spectrum: S_nu[Jy]=2.9nu^(-0.5)[GHz]. Probably that is the highest state spectrum from December 2007 (Trushkin et al. 2008, astro-ph:0810.3376) and by almost three times higher than in a quiet state of SS433.

  15. CoordSS: An Ontology Framework for Heterogeneous Networks Experimentation

    Directory of Open Access Journals (Sweden)

    V. Nejkovic

    2016-11-01

    Full Text Available Experimenting with HetNets environments is of importance because of the role that such environments have in next-generation cellular networks. In this paper, the CoordSS ontology experimentation framework is proposed with an aim to support experimenting with HetNets environments on wireless networking testbeds. In the framework, domain and system ontologies are adopted for formal representation of the knowledge about the context of the problem. This paper outlines implementation details of ontologies in the CoordSS experimentation framework. The synergy between semantic and cognitive computing is introduced as the theoretical foundation of the paper.

  16. Sosiaaliset ja vuorovaikutustaidot kulttuurituottajan työssä

    OpenAIRE

    Vauhkonen, Annukka

    2015-01-01

    Tämän tutkimuksen tarkoituksena on selvittää sosiaalisten ja vuorovaikutustaitojen merkitystä kulttuu-rituottajan työssä. Tutkimustavoitteena on kartoittaa, miten sosiaaliset ja vuorovaikutustaidot koetaan kulttuurituottajien keskuudessa työelämän erilaisissa tilanteissa. Tutkimuksessa tarkastellaan, mitkä ovat tärkeimmät sosiaaliset ja vuorovaikutustaidot tuottajan työssä ja mitä taitoja erityisesti tulisi kehittää. Kehittämistavoitteena on päivittää kulttuurituottajien työelämätaitoja ja li...

  17. ssDNA Binding Reveals the Atomic Structure of Graphene

    OpenAIRE

    Husale S; Sahoo S; Radenovic A; Traversi F; Annibale P; Kis A

    2010-01-01

    We used AFM to investigate the interaction of polyelectrolytes such as ssDNA and dsDNA molecules with graphene as a substrate. Graphene is an appropriate substrate due to its planarity, relatively large surfaces that are detectable via an optical microscope, and straightforward identification of the number of layers. We observe that in the absence of the screening ions deposited ssDNA will bind only to the graphene and not to the SiO2 substrate, confirming that the binding energy is mainly du...

  18. Työssäoppimisen ohjaamisen kehittäminen

    OpenAIRE

    Nenonen, Petri; Svala, Päivi

    2008-01-01

    Työssäoppiminen kuuluu ammatillisiin perusopintoihin. Työssäoppiminen on ohjattua ja arvioitua opiskelua työssäoppimispaikoilla ja siitä vastaa työssäoppimispaikoilla työpaikkaohjaaja. Työssäoppiminen on parhaimmillaan yhteistyötä ohjaavan opettajan ( koulu ), opiskelijan ja työpaikkaohjaajan ( yritys ) kanssa. Työssäoppimisen tavoitteena on edistää opiskelijan ammatillista kasvua ja oppimista sekä opiskelijan mahdollista työllistymistä opintojen jälkeen. Työssäoppimispaikalla on loistava mah...

  19. New EdF's three challenges; Les trois defis du nouvel EdF

    Energy Technology Data Exchange (ETDEWEB)

    Lepetit, V

    2005-11-15

    The opening of 15% of Electricite de France (EdF) capital to the private sector will allow the first world electric utility to raise 7 billions of euros of cash flow. EdF will have also to face the new European situation: a strained market, several consolidations to come and many competitors to deal with. EdF has to re-launch its production tool and to reinforce its interconnection capacities if it wants to stay the number one of power exports in Europe. The integration of Edison company (Italy) by EdF gives access to new gas capacities but other resources have to be found if EdF wants to enlarge its gas offer. A new concentration era is foreseen in Europe and EdF will have to seize the economic growth opportunities in particular in Europe and China. (J.S.)

  20. Katariina kirikus algab Geeniuste mäss / Kai Ilustrumm

    Index Scriptorium Estoniae

    Ilustrumm, Kai

    2004-01-01

    Briti Nõukogu toetusel Pärnus, Tartus ja Tallinnas toimuvatel inglise filmide päevadel "Geeniuste mäss" näidatakse ka 3 Lindsay Andersoni nn. Travise triloogia filmi "Kui..." (1968), "Oo, õnneseen!" (1973) ja "Ravila Britannia" (1982), kus Mick Travise rollis on Malcolm McDowell (1943). Lisatud kava

  1. Predictability of Joint Promotion Examinations in SS2 on Academic ...

    African Journals Online (AJOL)

    This research studied the predictability of joint SS2 promotion examinations of all the command secondary schools in Nigeria on academic performance of students in Senior School Certificate Examinations. The sample consists of 120 students selected at the Command Secondary School, Abakaliki and Command Day ...

  2. TED-Ed lessons & TED-Ed clubs: Educational activities to amplify students' voices

    Science.gov (United States)

    Villias, Georgios

    2017-04-01

    TED-Ed lessons and TED-Ed clubs are two powerful educational tools that can be used in today's school classrooms in order to create an educational environment that is engaging for the students and favors their active participation, created and fostered by TED-Ed. TED-Ed is TED's educational initiative, committed to create lessons worth sharing and amplify the voices and ideas of teachers and students around the world. TED-Ed animated lessons are fully organized lessons structured around an animated video that introduces new topics to learners in an exciting, thought-provoking way. These lessons have been created as a result of the cooperation between expert educators and animators and have been uploaded at the TED-Ed platform (http://ed.ted.com). On the other hand, TED-Ed Clubs are also an interesting way to offer students the chance, the voice and the opportunity to express their thoughts, engage actively on these matters and connect with each other, both at a local, as well as at an international level (http://ed.ted.com/clubs). By developing new TED-Ed lessons or by customizing appropriately existing animated TED-Ed lessons (translating, modifying the questions asked, introducing new discussion topics), I have created and implemented in my student-centered, didactic approach, a series of TED-ED animated lessons directly connected with the Greek national science syllabus that were used to spark students curiosity and initiate a further analytical discussion or introduce other relevant educational activities (http://gvillias.wixsite.com/education). Furthermore, at my school, we established Varvakeio TED-Ed Club, an environment that supports and empowers our students to research, develop and disseminate their own personal ideas that worth spreading. During the year, our members were inspired by watching TED talks presented by experts on their field on various different areas, including social, economical, environmental and technological-scientific issues. Our aim

  3. Canadian EdGEO National Workshop Program

    Science.gov (United States)

    Clinton, L. A.; Haidl, F. M.; Hymers, L. A.; van der Flier-Keller, E.

    2009-05-01

    Established in the early 1970s, EdGEO supports locally driven geosciences workshops for Canadian teachers. Workshops are organized by geoscientists and teachers, and typically have field, laboratory and classroom components. Grants of up to $3000 per workshop are available from the National EdGEO Program. By providing educational opportunities for today's teachers and, through them, their students, EdGEO seeks to cultivate a heightened awareness of our planet. EdGEO workshops provide teachers with potential fieldtrip sites for their students and the knowledge, enthusiasm and materials to inspire their students to engage in geoscience. Networking opportunities with local experts promote the importance of the geoscience profession. The expected result is an improved capacity on the part of Canadians to understand the Earth and to make informed decisions, especially with regard to the use of mineral and energy resources, the maintenance and remediation of the environment, and response to geological hazards. There exists a critical need to provide teachers with training and resources to tackle their Earth science curricula. In 2008, EdGEO supported fourteen workshops, with an unprecedented 521 teachers attending. These teachers then used our resources to reach an estimated 14,000 students during that single academic year. EdGEO workshops are locally driven and are therefore very diverse. Workshops are strongly tied to the provincial curriculum, focus on a specific geoscience topic, or may be largely field-based to demonstrate and practice how field activities could be incorporated into Earth science teaching. Many strive to include all of these important components. Geoscientists and teachers work collaboratively to develop and deliver EdGEO workshops to ensure that the activities can be effectively used in the classroom. The length of these professional development opportunities range from two-hour sessions to several days, and can generally accommodate up to twenty

  4. Susceptibility to Stress Corrosion Cracking of 254SMO SS

    Directory of Open Access Journals (Sweden)

    De Micheli Lorenzo

    2002-01-01

    Full Text Available The susceptibility to stress corrosion cracking (SCC of solubilized and sensitized 254SMO SS was studied in sodium chloride, and sodium fluoride solutions at 80 °C and sulfuric acid solutions in presence of sodium chloride at 25 °C. The influence of salt concentration, pH values and the addition of thiosulfate was examined. The susceptibility to SCC was evaluated by Slow Strain Rate Tests (SSRT, at 1.5 x 10-6 s-1 strain rate. The behavior of 254SMO was compared to those of AISI 316L SS and Hastelloy C276. 254SMO showed an excellent resistance to SCC in all conditions, except in the more acidic solutions (pH <= 1 where, in the sensitized conditions, intergranular stress corrosion cracking occurred.

  5. Ed Lorenz: Father of the 'Butterfly Effect'

    Indian Academy of Sciences (India)

    IAS Admin

    Ed Lorenz, rightfully acclaimed as the father of the 'Butterfly Effect', was an American math- ematician and meteorologist whose early work on weather prediction convinced the world at large about the unpredictability of weather. His seminal work on a simplified model for convec- tions in the atmosphere led to the modern ...

  6. (ED) by men in Edo state, Nigeria

    African Journals Online (AJOL)

    E. (Eshiobo) Irekpita

    2017-05-03

    May 3, 2017 ... of men in Edo state with regards to ED. The secondary objective .... Variables with sta- tistically significant correlation with IIEF score were respondents' location (P = 0.000), respondents' educational level (P = 0.000), number of wives (P ... between this variable and the level of education of the respondents.

  7. Clinical outcomes of ED patients with bandemia.

    Science.gov (United States)

    Shi, Eileen; Vilke, Gary M; Coyne, Christopher J; Oyama, Leslie C; Castillo, Edward M

    2015-07-01

    Although an elevated white blood cell count is a widely utilized measure for evidence of infection and an important criterion for evaluation of systemic inflammatory response syndrome, its component band count occupies a more contested position within clinical emergency medicine. Recent studies indicate that bandemia is highly predictive of a serious infection, suggesting that clinicians who do not appreciate the value of band counts may delay diagnosis or overlook severe infections. Whereas previous studies focused on determining the quantitative value of the band count (ie, determining sensitivity, threshold for bandemia, etc.), this study directs attention to patient-centered outcomes, hypothesizing that the degree of bandemia predisposes patients to subsequent negative clinical outcomes associated with underappreciated severe infections. This retrospective study of electronic medical records includes patients who initially presented to the emergency department (ED) with bandemia and were subsequently discharged from the ED. These patients were screened for repeat ED visits within 7 days and death within 30 days. In patients with severe bandemia who were discharged from the ED, there was a 20.9% revisit rate at 7 days and a 4.9% mortality rate at 30 days, placing severely bandemic patients at 5 times significantly greater mortality compared to nonbandemic patients (P = .032). Our review of patient outcomes suggests that the degree of bandemia, especially in the setting of concurrent tachycardia or fever, is associated with greater likelihood of negative clinical outcomes. Copyright © 2015 Elsevier Inc. All rights reserved.

  8. Atle Næss: Roten av minus en

    Directory of Open Access Journals (Sweden)

    Linda Hamrin Nesby

    2006-06-01

    Full Text Available Atle Næss har skrevet flere romaner, og to biografier om hhv Edevard Munch og Galileo Galilei. Med Roten av minus en kombinerer han roman- og biografigenren, og skriver både en kjærlighetshistorie, et utkast til en biografi og et riss av en selvbiografi som i sum blir til en roman om hvorvidt livet har den orden og nødvendighet som biografien er satt til å formidle

  9. Elastic-plastic analysis of the SS-3 tensile specimen

    Energy Technology Data Exchange (ETDEWEB)

    Majumdar, S. [Argonne National Lab., IL (United States)

    1998-09-01

    Tensile tests of most irradiated specimens of vanadium alloys are conducted using the miniature SS-3 specimen which is not ASTM approved. Detailed elastic-plastic finite element analysis of the specimen was conducted to show that, as long as the ultimate to yield strength ratio is less than or equal to 1.25 (which is satisfied by many irradiated materials), the stress-plastic strain curve obtained by using such a specimen is representative of the true material behavior.

  10. Social class and survival on the S.S. Titanic.

    Science.gov (United States)

    Hall, W

    1986-01-01

    Passengers' chances of surviving the sinking of the S.S. Titanic were related to their sex and their social class: females were more likely to survive than males, and the chances of survival declined with social class as measured by the class in which the passenger travelled. The probable reasons for these differences in rates of survival are discussed as are the reasons accepted by the Mersey Committee of Inquiry into the sinking.

  11. Sustainable Mechanism to Reduce Emergency Department (ED) Length of Stay: The Use of ED Holding (ED Transition) Orders to Reduce ED Length of Stay.

    Science.gov (United States)

    Haydar, Samir A; Strout, Tania D; Baumann, Michael R

    2016-07-01

    The objective was to evaluate the effect of an emergency clinician-initiated "ED admission holding order set" on emergency department (ED) treatment times and length of stay (LOS). We further describe the impact of a performance improvement strategy with sequential plan-do-study-act (PDSA) cycles used to influence the primary outcome measures, ED LOS, and disposition decision to patient gone (DDTPG) time, for admitted patients. We developed and implemented an expedited, emergency physician-facilitated admission protocol that bypassed typical inpatient workflows requiring inpatient evaluations prior to the placement of admission orders. During the 48-month study period, ED flow metrics generated during the care of 27,580 admissions from the 24-month period prior to the intervention were compared to the 29,978 admissions that occurred during the 24-month period following the intervention. The intervention was the result of an in-depth, five-phase PDSA cycle quality improvement intervention evaluating ED flow, which identified the requirement of bedside inpatient evaluations prior admission order placement as being a "non-value-added" activity. ED output flow metrics evaluating the admission process were tracked for 24 months following the intervention and were compared to the 24 months prior. The use of an emergency physician-initiated admission holding order protocol resulted in sustainable reductions in ED LOS when comparing the 2 years prior to the intervention, with median LOS of 410 (interquartile range [IQR] = 295 to 543) and 395 (IQR = 283 to 527) minutes, to the 2 calendar years following the intervention, with the median LOS of 313 (IQR = 21 to 431) and 316 (IQR = 224 to 438) minutes, respectively. This overall reduction in ED LOS of nearly 90 minutes was found to be primarily the result of a decrease in the time from the emergency physician's admitting DDTPG times with median times of 219 (IQR = 150 to 306) and 200 (IQR = 136 to 286) minutes for the 2 years

  12. Risk factors for ED use among homeless veterans.

    Science.gov (United States)

    Tsai, Jack; Rosenheck, Robert A

    2013-05-01

    Despite national concern about homeless veterans, there has been little examination of their use of emergency department (ED) services. This study examines factors related to the use of ED services in the Veterans Affairs (VA) healthcare system, where insurance is not a barrier to ambulatory healthcare. National VA administrative data from fiscal year 2010 are used to describe the proportions of ED users among homeless and domiciled VA patients. A case-control design is then used to compare homeless ED and non-ED users on sociodemographic and clinical correlates, as well as use of ambulatory care and psychotropic medications. Sixteen percent of domiciled VA patients used EDs at least once during the year and 1% were frequent ED users (>4 ED visits) compared to 45% of homeless VA patients, 10% who were frequent ED users. Among homeless VA patients, those who used EDs were more likely to have a range of psychiatric and medical conditions, and had more service visits and psychotropic medication prescriptions than non-ED users. Multivariate analyses suggest their risk for psychiatric and medical conditions increase their likelihood of using ED services. The high rate of ED use among homeless veterans is associated with significant morbidity, but also greater use of ambulatory care and psychotropics suggesting their ED use may reflect unmet psychosocial needs. Published by Elsevier Inc.

  13. 32 CFR Appendix E to Part 246 - Stars and Stripes (S&S) Board of Directors

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Stars and Stripes (S&S) Board of Directors E... DEFENSE (CONTINUED) MISCELLANEOUS STARS AND STRIPES (S&S) NEWSPAPER AND BUSINESS OPERATIONS Pt. 246, App. E Appendix E to Part 246—Stars and Stripes (S&S) Board of Directors A. Organization and Management...

  14. Investigation on Ti6Al4V-V-Cr-Fe-SS316 Multi-layers Metallic Structure Fabricated by Laser 3D Printing.

    Science.gov (United States)

    Li, Wei; Liou, Frank; Newkirk, Joseph; Taminger, Karen M Brown; Seufzer, William J

    2017-08-11

    Joining titanium alloy and stainless steel is becoming an urgent need since their outstanding mechanical properties can be utilized integratedly. However, direct fusion joining of Ti6Al4V to SS316 can cause brittle Ti-Fe intermetallics which compromise join bonds' mechanical properties. In this research, Laser 3D Printing was applied to explore a new Ti6Al4V to SS316 multi-metallic structure. A novel filler transition route was introduced (Ti6Al4V → V → Cr → Fe → SS316) to avoid the Ti-Fe intermetallics. Two experimental cases were performed for comparison to evaluate this novel route's effect. In the first case, SS316 layer was directly deposited on Ti6Al4V substrate by laser 3D printing, but the sample cracked in the printing process. Then fracture morphology, phase identification, and micro-hardness were analyzed. In the second case, a multi-metallic structure was fabricated via laser 3D printing following the transition route. Microstructure characterization and composition distribution were analyzed via scanning electron microscope(SEM) and energy dispersive spectrometry(EDS). x-ray diffraction(XRD) tests demonstrated the intermetallics were effectively avoided following the transition route. Vickers hardness number(VHN) showed no significant hard brittle phases in the sample. Comparing with directly depositing SS316 on Ti6Al4V, the usage of the novel transition route can eliminate the intermetallics effectively. These research results are good contributions in joining titanium alloy and stainless steel.

  15. LOFAR 150-MHz observations of SS 433 and W 50

    Science.gov (United States)

    Broderick, J. W.; Fender, R. P.; Miller-Jones, J. C. A.; Trushkin, S. A.; Stewart, A. J.; Anderson, G. E.; Staley, T. D.; Blundell, K. M.; Pietka, M.; Markoff, S.; Rowlinson, A.; Swinbank, J. D.; Horst, A. J. van der; Bell, M. E.; Breton, R. P.; Carbone, D.; Corbel, S.; Eislöffel, J.; Falcke, H.; Grießmeier, J.-M.; Hessels, J. W. T.; Kondratiev, V. I.; Law, C. J.; Molenaar, G. J.; Serylak, M.; Stappers, B. W.; van Leeuwen, J.; Wijers, R. A. M. J.; Wijnands, R.; Wise, M. W.; Zarka, P.

    2018-01-01

    We present LOFAR high-band data over the frequency range 115-189 MHz for the X-ray binary SS 433, obtained in an observing campaign from 2013 February - 2014 May. Our results include a deep, wide-field map, allowing a detailed view of the surrounding supernova remnant W 50 at low radio frequencies, as well as a light curve for SS 433 determined from shorter monitoring runs. The complex morphology of W 50 is in excellent agreement with previously published higher-frequency maps; we find additional evidence for a spectral turnover in the eastern wing, potentially due to foreground free-free absorption. Furthermore, SS 433 is tentatively variable at 150 MHz, with both a debiased modulation index of 11 per cent and a χ2 probability of a flat light curve of 8.2 × 10-3. By comparing the LOFAR flux densities with contemporaneous observations carried out at 4800 MHz with the RATAN-600 telescope, we suggest that an observed ˜0.5-1 Jy rise in the 150-MHz flux density may correspond to sustained flaring activity over a period of approximately six months at 4800 MHz. However, the increase is too large to be explained with a standard synchrotron bubble model. We also detect a wealth of structure along the nearby Galactic plane, including the most complete detection to date of the radio shell of the candidate supernova remnant G 38.7-1.4. This further demonstrates the potential of supernova remnant studies with the current generation of low-frequency radio telescopes.

  16. VirE2: a unique ssDNA-compacting molecular machine.

    Directory of Open Access Journals (Sweden)

    Wilfried Grange

    2008-02-01

    Full Text Available The translocation of single-stranded DNA (ssDNA across membranes of two cells is a fundamental biological process occurring in both bacterial conjugation and Agrobacterium pathogenesis. Whereas bacterial conjugation spreads antibiotic resistance, Agrobacterium facilitates efficient interkingdom transfer of ssDNA from its cytoplasm to the host plant cell nucleus. These processes rely on the Type IV secretion system (T4SS, an active multiprotein channel spanning the bacterial inner and outer membranes. T4SSs export specific proteins, among them relaxases, which covalently bind to the 5' end of the translocated ssDNA and mediate ssDNA export. In Agrobacterium tumefaciens, another exported protein-VirE2-enhances ssDNA transfer efficiency 2000-fold. VirE2 binds cooperatively to the transferred ssDNA (T-DNA and forms a compact helical structure, mediating T-DNA import into the host cell nucleus. We demonstrated-using single-molecule techniques-that by cooperatively binding to ssDNA, VirE2 proteins act as a powerful molecular machine. VirE2 actively pulls ssDNA and is capable of working against 50-pN loads without the need for external energy sources. Combining biochemical and cell biology data, we suggest that, in vivo, VirE2 binding to ssDNA allows an efficient import and pulling of ssDNA into the host. These findings provide a new insight into the ssDNA translocation mechanism from the recipient cell perspective. Efficient translocation only relies on the presence of ssDNA binding proteins in the recipient cell that compacts ssDNA upon binding. This facilitated transfer could hence be a more general ssDNA import mechanism also occurring in bacterial conjugation and DNA uptake processes.

  17. Lanka ja rukousnauha- hengellisyys hoitotyössä.

    OpenAIRE

    Ahvonen, Senja

    2011-01-01

    Opinnäytetyön tavoite oli kartoittaa, miten ihmisen hengellisyys ymmärretään ja nähdään hoitotyössä. Hoitajien näkökulmasta tutkittiin ammatillista valmiutta kohdata potilaan hengellisiä tarpeita ja sitä, miten uskontojen monimuotoisuus nähdään osana tulevaisuuden hoitotyötä. Potilaan näkökulmasta hengellisyyttä tarkasteltiin ihmisen kokonaisuuteen kuuluvana alueena ja pyrittiin määrittelemään, tulevatko heidän hengelliset tarpeensa kohdatuksi. Tutkimus suoritettiin laadullisena tutkim...

  18. Osler usque ad mare: the SS William Osler.

    Science.gov (United States)

    Bryan, C S; Fransiszyn, M

    1999-10-05

    William Osler's connections with the sea included a strong family history of seafaring, his own transatlantic crossings (of which there were at least 32) and the occasional use of nautical imagery in his inspirational writings. An unusual Oslerian connection with the sea emerged after his death in the form of a World War II Liberty ship. Through the SS William Osler and its sister ships, Osler was symbolically reunited with colleagues associated with the early days of the Johns Hopkins Hospital. The William Osler circumnavigated the globe in 1943 without engaging the enemy. She was then converted into an army hospital ship and renamed the USHS Wisteria.

  19. edX e-learning course development

    CERN Document Server

    Gilbert, Matthew A

    2015-01-01

    If you are an educator creating a course for edX or a corporate trainer using Open edX for large-scale learning and development initiatives, then edX E-Learning Course Development is the ideal book for you.

  20. La Costituzione Islandese: storia ed evoluzione

    Directory of Open Access Journals (Sweden)

    Fabio Quartino

    2011-03-01

    Full Text Available "La Costituzione Islandese: storia ed evoluzione" is a degree thesis defended on 15th July 2009 by Fabio Quartino under the supervision of Prof. Andrea Canepa of the University of Genoa, Italy. It offers an extensive overview of Icelandic history aimed at finding out the roots of the nation's constitutional order, which is currently in the process of being revised by the first ever elected constitutional assembly in the life of the country. Fabio Quartino's work wishes to be a source of useful information for Italian-speaking scholars who have an interest in Iceland's constitutional history and may not have access to comprehensive overviews in their native tongue.

  1. Perceptions of empowerment among ED nurses.

    Science.gov (United States)

    DeVivo, Diane; Quinn Griffin, Mary T; Donahue, Moreen; Fitzpatrick, Joyce J

    2013-11-01

    Nurses' perceptions of empowerment have been linked to a number of variables in the hospital workplace, including job satisfaction, autonomy, and work effectiveness. Yet there have been no previous studies of perceptions of empowerment specifically among emergency department (ED) nurses. Registered nurses (RNs) employed in the EDs of 6 hospitals in a major health care system in the eastern United States were surveyed regarding their perceptions of empowerment. Of the 240 RNs eligible to participate, there were 167 usable surveys. There was a moderate level of empowerment among the RNs who participated, consistent with the level of empowerment reported in several other studies of staff nurses and nurses in other positions. The moderate level of empowerment in this sample may be attributed to the many opportunities for RN involvement in the hospitals within this health care system. Nurse leaders can initiate programs focused on enhancing RN perceptions of empowerment. In addition, there is a need for further research among RNs with different specialty preparation. Copyright © 2013. Published by Mosby, Inc.

  2. Optical, UV, and EUV Oscillations of SS Cygni in Outburst

    Science.gov (United States)

    Mauche, Christopher W.

    2004-07-01

    I provide a review of observations in the optical, UV (HST), and EUV (EUVE and Chandra LETG) of the rapid periodic oscillations of nonmagnetic, disk-accreting, high mass-accretion rate cataclysmic variables (CVs), with particular emphasis on the dwarf nova SS Cyg in outburst. In addition, I drawn attention to a correlation, valid over nearly six orders of magnitude in frequency, between the frequencies of the quasi-periodic oscillations (QPOs) of white dwarf, neutron star, and black hole binaries. This correlation identifies the high frequency quasi-coherent oscillations (so-called ``dwarf nova oscillations'') of CVs with the kilohertz QPOs of low mass X-ray binaries (LMXBs), and the low frequency and low coherence QPOs of CVs with the horizontal branch oscillations (or the broad noise component identified as such) of LMXBs. Assuming that the same mechanisms produce the QPOs of white dwarf, neutron star, and black hole binaries, this correlation has important implications for QPO models.

  3. Performance Analysis of HF Band FB-MC-SS

    Energy Technology Data Exchange (ETDEWEB)

    Hussein Moradi; Stephen Andrew Laraway; Behrouz Farhang-Boroujeny

    2016-01-01

    Abstract—In a recent paper [1] the filter bank multicarrier spread spectrum (FB-MC-SS) waveform was proposed for wideband spread spectrum HF communications. A significant benefit of this waveform is robustness against narrow and partial band interference. Simulation results in [1] demonstrated good performance in a wideband HF channel over a wide range of conditions. In this paper we present a theoretical analysis of the bit error probably for this system. Our analysis tailors the results from [2] where BER performance was analyzed for maximum ration combining systems that accounted for correlation between subcarriers and channel estimation error. Equations are give for BER that closely match the simulated performance in most situations.

  4. Automaatioteknologia hyvinvointia edistämässä

    OpenAIRE

    Tommiska, Janika

    2016-01-01

    Automaatioteknologia hyvinvointialalla saa koko ajan lisää tilaa. Sovellusten ja laitteiden käyttö on tullut ihmisille tutummaksi ja uusia ideoita syntyy jatkuvasta. Automaatioteknologian tarkoituksena ei ole vähentää työntekijöiden määrää vaan vapauttaa heidän aikaansa, jotta he voisivat käyttää sitä yhä enemmän potilaiden henkilökohtaiseen ohjaamiseen ja auttamiseen. Opinnäytetyön tarkoituksena on pohtia automaatioteknologioita hyvinvoinnin edistämisen näkökulmasta. Työssä määritellään...

  5. PCI-SS: MISO dynamic nonlinear protein secondary structure prediction

    Directory of Open Access Journals (Sweden)

    Aboul-Magd Mohammed O

    2009-07-01

    Full Text Available Abstract Background Since the function of a protein is largely dictated by its three dimensional configuration, determining a protein's structure is of fundamental importance to biology. Here we report on a novel approach to determining the one dimensional secondary structure of proteins (distinguishing α-helices, β-strands, and non-regular structures from primary sequence data which makes use of Parallel Cascade Identification (PCI, a powerful technique from the field of nonlinear system identification. Results Using PSI-BLAST divergent evolutionary profiles as input data, dynamic nonlinear systems are built through a black-box approach to model the process of protein folding. Genetic algorithms (GAs are applied in order to optimize the architectural parameters of the PCI models. The three-state prediction problem is broken down into a combination of three binary sub-problems and protein structure classifiers are built using 2 layers of PCI classifiers. Careful construction of the optimization, training, and test datasets ensures that no homology exists between any training and testing data. A detailed comparison between PCI and 9 contemporary methods is provided over a set of 125 new protein chains guaranteed to be dissimilar to all training data. Unlike other secondary structure prediction methods, here a web service is developed to provide both human- and machine-readable interfaces to PCI-based protein secondary structure prediction. This server, called PCI-SS, is available at http://bioinf.sce.carleton.ca/PCISS. In addition to a dynamic PHP-generated web interface for humans, a Simple Object Access Protocol (SOAP interface is added to permit invocation of the PCI-SS service remotely. This machine-readable interface facilitates incorporation of PCI-SS into multi-faceted systems biology analysis pipelines requiring protein secondary structure information, and greatly simplifies high-throughput analyses. XML is used to represent the input

  6. Stanislaw Widlak (ed.), Italianità ed Italianistica nell'Europa Centrale ...

    African Journals Online (AJOL)

    User

    nel futuro ad intensi scambi e collaborazioni tra italianisti che sono necessari allo sviluppo ed alla crescita di ogni disciplina. I partecipanti al congresso sono stati circa una cinquantina, tra studiosi e studenti, provenienti da una ventina di università e da una dozzina di paesi. Gli argomenti trattati nei loro interventi sono stati ...

  7. Turvallisuuskulttuuri säteilyn lääketieteellisessä käytössä ja hoitotyössä : kirjallisuuskatsaus

    OpenAIRE

    Hallila, Mikko; Heinänen, Mikko; Suopanki, Sari

    2011-01-01

    Opinnäytetyömme tarkoituksena oli kartoittaa systemaattisen kirjallisuushaun avulla, mitä on turvallisuuskulttuuri säteilyn lääketieteellisessä käytössä ja hoitotyössä. Teimme kirjallisuushaun käyttämällä eri tietokantoja ennalta määriteltyjen hakusanojen pohjalta. Työmme eteni systemaattisen kirjallisuuskatsauksen rakenteen mukaisesti. Kirjallisuushaussa löytyi lopulta kolme kriteerimme täyttävää artikkelia, jotka käsittelivät turvallisuuskulttuuria säteilyn lääketieteellisessä käytössä....

  8. Citar e referenciar : APA 6ª ed.

    OpenAIRE

    Marcos, Isabel Marques

    2016-01-01

    A elaboração correta dos trabalhos científicos pressupõe o respeito por um conjunto de princípios normativos de citação e referenciação bibliográfica que salvaguardam os autores das criações intelectuais do uso indevido das mesmas. Partindo de três conceitos: citação, referência bibliográfica e lista de referências apresentam-se as normas da APA 6ª ed. nos seus elementos essenciais. Estas normas, da área da informação e documentação, definem regras para a normalização das referências bibliogr...

  9. Giocare, tra violenza ed ecologia della mente.

    Directory of Open Access Journals (Sweden)

    Enrico Euli

    2008-07-01

    Full Text Available L'articolo riflette sulle relazioni tra comunicazione, violenza e gioco, nel tentativo di rintracciare tra loro convergenze, affinità e contrasti. Le nostre relazioni, infatti, ci immettono inerzialmente in dilemmi e paradossi di così alta complessità da rendere spesso inadeguati gli strumenti 'logici' ed 'analitici': dal che può derivare un senso di impotenza, rabbia, paralisi, ma anche un desiderio potente di controllo e di soluzione degli eventi. Entrambe queste strade ci conducono dentro le false alternative della violenza. La cultura del gioco, invece, ci propone (attraverso le sue pratiche, ma anche negli scritti teorici di Freud, Winnicott, Bateson, qui riassunti e rivisitati una modalità più creativa e nonviolenta, più prossima ad un' ecologia della mente e ad un'estetica delle emozioni.

  10. Työmaatoimintojen kehittäminen Maanrakennus Ahti Virtanen Oy:ssä

    OpenAIRE

    Tammisto, Mikko

    2011-01-01

    ssä opinnäytetyössä käsiteltiin työmaatoimintojen kehittämistä Maanrakennus Ahti Virtanen Oy:ssä. Aihekokonaisuuksiin kuului työturvallisuus, toimintatapa ja riskienhallinta. Työstä rajattiin pois yrityksen hallintoon ja talouteen liittyvät asiat sekä työmaan aikataulu- ja kustannushallintaan liittyvät kokonaisuudet. Työn tarkoituksena oli luoda perusteet yrityksen toimintajärjestelmän kehittämiselle. Työssä koottiin yhteen teoria-asiaa, jonka jälkeen esiteltiin Maanrakennus Ahti Virtan...

  11. [Gene expression of AAV-ITR ssDNA mini vector in skeletal muscle of mice].

    Science.gov (United States)

    Zhu, Dongqin; Zhang, Yun; Liu, Xiaomei; Zhang, Chun

    2014-11-01

    AAV-ITR single strand DNA mini vector (AAV-ITR ssDNA mini vector) is a novel gene expression vector based on AAV-ITR. We have shown efficient gene expression of AAV-ITR ssDNA mini vector in HEK 293T. Here, we studied the efficacy of gene expression of AAV-ITR ssDNA mini vector in vivo. We injected the skeletal muscle of ICR mice separately with equal molars of AAV-ITR ssDNA mini vector, ITR mutated AAV-ITR single strand DNA mini vector (AAV-ITRmm ssDNA mutant vector), AAV-ITR dsDNA and pUC57-minivector-GFP, combined with TurboFect. Florescence microscope analysis of skeletal muscle section shows that AAV-ITR ssDNA mini vector had higher expression efficiency and longer expression period. We extracted DNA from the muscle three months after injection and quantified three vectors by Real-time PCR. RT-PCR analysis shows that there were highest copy numbers of AAV-ITR ssDNA mini vector existing in muscle. Stable existing of AAV- TR ssDNA mini vector in muscle could be the molecular basis of long term gene expression of the vector. The results suggest that AAV-ITR ssDNA mini vector might be a promising vector for gene therapy.

  12. Multimodal pyrethroid resistance in malaria vectors, Anopheles gambiae s.s., Anopheles arabiensis, and Anopheles funestus s.s. in western Kenya.

    Science.gov (United States)

    Kawada, Hitoshi; Dida, Gabriel O; Ohashi, Kazunori; Komagata, Osamu; Kasai, Shinji; Tomita, Takashi; Sonye, George; Maekawa, Yoshihide; Mwatele, Cassian; Njenga, Sammy M; Mwandawiro, Charles; Minakawa, Noboru; Takagi, Masahiro

    2011-01-01

    Anopheles gambiae s.s., Anopheles arabiensis, and Anopheles funestus s.s. are the most important species for malaria transmission. Pyrethroid resistance of these vector mosquitoes is one of the main obstacles against effective vector control. The objective of the present study was to monitor the pyrethroid susceptibility in the 3 major malaria vectors in a highly malaria endemic area in western Kenya and to elucidate the mechanisms of pyrethroid resistance in these species. Gembe East and West, Mbita Division, and 4 main western islands in the Suba district of the Nyanza province in western Kenya were used as the study area. Larval and adult collection and bioassay were conducted, as well as the detection of point mutation in the voltage-gated sodium channel (1014L) by using direct DNA sequencing. A high level of pyrethroid resistance caused by the high frequency of point mutations (L1014S) was detected in An. gambiae s.s. In contrast, P450-related pyrethroid resistance seemed to be widespread in both An. arabiensis and An. funestus s.s. Not a single L1014S mutation was detected in these 2 species. A lack of cross-resistance between DDT and permethrin was also found in An. arabiensis and An. funestus s.s., while An. gambiae s.s. was resistant to both insecticides. It is noteworthy that the above species in the same area are found to be resistant to pyrethroids by their unique resistance mechanisms. Furthermore, it is interesting that 2 different resistance mechanisms have developed in the 2 sibling species in the same area individually. The cross resistance between permethrin and DDT in An. gambiae s.s. may be attributed to the high frequency of kdr mutation, which might be selected by the frequent exposure to ITNs. Similarly, the metabolic pyrethroid resistance in An. arabiensis and An. funestus s.s. is thought to develop without strong selection by DDT.

  13. Multimodal pyrethroid resistance in malaria vectors, Anopheles gambiae s.s., Anopheles arabiensis, and Anopheles funestus s.s. in western Kenya.

    Directory of Open Access Journals (Sweden)

    Hitoshi Kawada

    Full Text Available Anopheles gambiae s.s., Anopheles arabiensis, and Anopheles funestus s.s. are the most important species for malaria transmission. Pyrethroid resistance of these vector mosquitoes is one of the main obstacles against effective vector control. The objective of the present study was to monitor the pyrethroid susceptibility in the 3 major malaria vectors in a highly malaria endemic area in western Kenya and to elucidate the mechanisms of pyrethroid resistance in these species. Gembe East and West, Mbita Division, and 4 main western islands in the Suba district of the Nyanza province in western Kenya were used as the study area. Larval and adult collection and bioassay were conducted, as well as the detection of point mutation in the voltage-gated sodium channel (1014L by using direct DNA sequencing. A high level of pyrethroid resistance caused by the high frequency of point mutations (L1014S was detected in An. gambiae s.s. In contrast, P450-related pyrethroid resistance seemed to be widespread in both An. arabiensis and An. funestus s.s. Not a single L1014S mutation was detected in these 2 species. A lack of cross-resistance between DDT and permethrin was also found in An. arabiensis and An. funestus s.s., while An. gambiae s.s. was resistant to both insecticides. It is noteworthy that the above species in the same area are found to be resistant to pyrethroids by their unique resistance mechanisms. Furthermore, it is interesting that 2 different resistance mechanisms have developed in the 2 sibling species in the same area individually. The cross resistance between permethrin and DDT in An. gambiae s.s. may be attributed to the high frequency of kdr mutation, which might be selected by the frequent exposure to ITNs. Similarly, the metabolic pyrethroid resistance in An. arabiensis and An. funestus s.s. is thought to develop without strong selection by DDT.

  14. Completing EdTPA: TSOL Candidate Performance and Reflection

    Science.gov (United States)

    Micek, Timothy A.

    2017-01-01

    edTPA is a pre-service assessment process designed to determine if a new teacher is ready for the job. edTPA is part of a national movement towards the use of performance assessments in teacher education. As of 2014, 41 states (a) require a state-approved performance assessment like edTPA for program completion or for state licensure and/or state…

  15. 'Psych ed' helps speed throughput time by 9%.

    Science.gov (United States)

    2006-02-01

    Adequate staffing, proper location, and design of psychiatric ED services area are critical to successful implementation. One ED cut average throughput time by 9%. Have mental health workers available to determine the level of care each patient needs. Have your psych area set apart from the main ED to avoid excessive noise and confusion. Have a minimal amount of equipment in the rooms so that patients cannot harm themselves.

  16. Optimization of carboxymethylcellulase production from Bacillus amyloliquefaciens SS35.

    Science.gov (United States)

    Singh, Shuchi; Moholkar, Vijayanand S; Goyal, Arun

    2014-08-01

    In this paper, we have attempted optimization of production of enzyme carboxymethylcellulase or endoglucanase from the bacterium Bacillus amyloliquefaciens SS35. Optimization has been carried out in two stages using statistical experimental design, viz. medium optimization and optimization of fermentation parameters. For medium optimization, Plackett-Burman design followed by central composite design (CCD) was used, while for optimization of fermentation parameters one-variable-at-a-time method followed by CCD was used. Carbon and nitrogen sources in the medium have been revealed to be the significant factors for enzyme production (carboxymethylcellulose 18.05 g/L; yeast extract 8 g/L and peptone 2 g/L). The inorganic salts have been found to be insignificant components of medium. Optimum fermentation parameters for optimized medium were: initial medium pH 5.65, incubation temperature = 40 °C, shaking speed = 120 rpm, and inoculum size = 6.96 %, v/v. Interestingly, the influence of all four parameters was almost independent with no interlinks. Secondly, the overall effect of all parameters was also low, as indicated by linear, square and interaction regression coefficients that were at least one order of magnitude lower than the intercept in the model equation. These results essentially meant that medium components dominate overall enzyme production process in comparison to fermentation parameters.

  17. Detection of Mycobacterium avium ss. Paratuberculosis in Blau Syndrome Tissues

    Directory of Open Access Journals (Sweden)

    C. Thomas Dow

    2010-01-01

    Full Text Available Background and Aim of the Work. Blau syndrome is an inherited granulomatous inflammatory disorder with clinical findings of uveitis, arthritis, and dermatitis. Although rare, Blau syndrome shares features with the more common diseases sarcoidosis and Crohn's disease. The clinical findings of Blau syndrome are indistinguishable from juvenile sarcoidosis; the mutations of Blau syndrome are on the same gene of chromosome 16 (CARD15 that confers susceptibility to Crohn's disease. The product of this gene is part of the innate immune system. Mycobacterium avium ss. paratuberculosis (MAP is the putative cause of Crohn's disease and has been implicated as a causative agent of sarcoidosis. Methods. Archival tissues of individuals with Blau syndrome were tested for the presence of MAP. Results. DNA evidence of MAP was detected in all of the tissues. Conclusions. This article finds that MAP is present in Blau syndrome tissue and postulates that it has a causal role. The presence of MAP in Blau syndrome—an autosomal dominant, systemic inflammatory disease—connects genetic and environmental aspects of “autoimmune” disease.

  18. Detection of Mycobacterium avium ss. Paratuberculosis in Blau Syndrome Tissues.

    Science.gov (United States)

    Dow, C Thomas; Ellingson, Jay L E

    2010-06-20

    Background and Aim of the Work. Blau syndrome is an inherited granulomatous inflammatory disorder with clinical findings of uveitis, arthritis, and dermatitis. Although rare, Blau syndrome shares features with the more common diseases sarcoidosis and Crohn's disease. The clinical findings of Blau syndrome are indistinguishable from juvenile sarcoidosis; the mutations of Blau syndrome are on the same gene of chromosome 16 (CARD15) that confers susceptibility to Crohn's disease. The product of this gene is part of the innate immune system. Mycobacterium avium ss. paratuberculosis (MAP) is the putative cause of Crohn's disease and has been implicated as a causative agent of sarcoidosis. Methods. Archival tissues of individuals with Blau syndrome were tested for the presence of MAP. Results. DNA evidence of MAP was detected in all of the tissues. Conclusions. This article finds that MAP is present in Blau syndrome tissue and postulates that it has a causal role. The presence of MAP in Blau syndrome-an autosomal dominant, systemic inflammatory disease-connects genetic and environmental aspects of "autoimmune" disease.

  19. Pore formation by T3SS translocators: liposome leakage assay.

    Science.gov (United States)

    Faudry, Eric; Perdu, Caroline; Attrée, Ina

    2013-01-01

    Gram-negative bacteria utilize a dedicated membrane-embedded apparatus, the type III secretion system (T3SS), to inject proteins into host cells. The passage of the proteins across the target membrane is accomplished by a proteinaceous pore-the translocon-formed within the host-cell cytoplasmic membrane. Translocators bound to their chaperones can be expressed in Escherichia coli and subsequently dissociated from the chaperone by guanidine treatment. The pore formation properties of the translocators can then be studied by an in-vitro liposome leakage assay. Sulforhodamine-B is encapsulated within lipid vesicles during liposome preparation. At high concentration, this fluorochrome exhibits self-quenching limiting fluorescence emission. Upon pore formation, liposome leakage leads to the dilution of Sulforhodamine-B in the medium and fluorescence emission increases. Alternatively, fluorochromes coupled to large dextran molecules can be encapsulated in order to estimate pore dimensions. Here we describe protein expression and purification, dye-liposome preparation, and leakage assay conditions.

  20. Optical, UV, and EUV Oscillations of SS Cygni in Outburst

    Energy Technology Data Exchange (ETDEWEB)

    Mauche, C W

    2003-12-19

    I provide a review of observations in the optical, UV (HST), and EUV (EUVE and Chandra LETG) of the rapid periodic oscillations of nonmagnetic, disk-accreting, high mass-accretion rate cataclysmic variables (CVs), with particular emphasis on the dwarf nova SS Cyg in outburst. In addition, I drawn attention to a correlation, valid over nearly six orders of magnitude in frequency, between the frequencies of the quasi-periodic oscillations (QPOs) of white dwarf, neutron star, and black hole binaries. This correlation identifies the high frequency quasi-coherent oscillations (so-called ''dwarf nova oscillations'') of CVs with the kilohertz QPOs of low mass X-ray binaries (LMXBs), and the low frequency and low coherence QPOs of CVs with the horizontal branch oscillations (or the broad noise component identified as such) of LMXBs. Assuming that the same mechanisms produce the QPOs of white dwarf, neutron star, and black hole binaries, this correlation has important implications for QPO models.

  1. An association between snowfall and ED presentation of cardiac arrest.

    Science.gov (United States)

    Spitalnic, S J; Jagminas, L; Cox, J

    1996-10-01

    Studies from several cities have reported increased cardiovascular mortality associated with snowfall; whether this weather also results in increased emergency department (ED) presentation of cardiac arrests is not known. A retrospective review was conducted of cardiac arrest patients presenting to a New England ED during the months of October through May, from 1991 to 1994. Comparing daily frequency of cardiac arrest patients with climactic data, a 27% increase was observed in the frequency of cardiac arrest presentation to the ED on days with snowfall (P = .0004). ED physicians and staff should anticipate an increased frequency of cardiac arrest patients on days with snowfall.

  2. Combining the antimesothelin immunotoxin SS1P with the BH3-mimetic ABT-737 induces cell death in SS1P-resistant pancreatic cancer cells.

    Science.gov (United States)

    Hollevoet, Kevin; Antignani, Antonella; Fitzgerald, David J; Pastan, Ira

    2014-01-01

    SS1P is an antimesothelin recombinant immunotoxin (RIT). Pancreatic ductal adenocarcinoma (PDAC) cell lines are resistant to SS1P, despite high mesothelin expression. The aim of this study is to examine whether combining SS1P and BH3-mimetic ABT-737 induces cell death in a panel of PDAC cell lines. ABT-737 binds and neutralizes several antiapoptotic BCL2 family proteins, but has a low affinity for the short-lived MCL1 and BCL2A1. SS1P inhibits protein synthesis, which has shown to downregulate MCL1. PDAC cell lines KLM-1, BxPc-3, and Panc 3.014 were resistant to SS1P or ABT-737 alone. Combining both compounds led to a significant increase in cell death. After 48 hours of treatment, cell death was observed in 92% of KLM-1, 55% of BxPc-3, and 23% of Panc 3.014 cells. Panc 3.014 had the highest number of mesothelin-binding sites (92×10(3)), followed by KLM-1 (58×10(3)) and BxPc-3 (3×10(3)). ABT-737 had no effect on SS1P internalization, but enhanced SS1P-induced protein synthesis inhibition significantly in KLM-1, to a lesser extent in BxPc-3, and very little in Panc 3.014. SS1P alone or in combination with ABT-737 downregulated MCL1 in KLM-1 and BxPc-3, but not in Panc 3.014. Similar observations were made for BCL2A1, which had the highest levels in Panc 3.014. Compared with KLM-1, Panc 3.014, and BxPc-3 also had lower proapoptotic BAK and a trend toward higher MCL1. Proapoptotic BAX was similar in KLM-1 and BxPc-3, but lower in Panc 3.014. In conclusion, combining SS1P with ABT-737 overcomes SS1P-resistance in PDAC, although to a variable extent. The efficacy of the combination is mainly associated with the RIT-associated inhibition of protein synthesis and the ability to downregulate MCL1 and BCL2A1, while levels of other key apoptotic proteins may also be important. Our data support the combination of an RIT and a BH3-mimetic, and identify factors that potentially limit the efficacy of such therapeutic approach.

  3. Minor injury attendance times to the ED.

    LENUS (Irish Health Repository)

    Conlon, Ciaran

    2009-07-01

    The Health Service Executive (HSE) highlights the need for effective patient throughput and management, whilst providing appropriate staffing and therapeutic interventions. It acknowledges that patient need is integral to the development of a nurse led service and advocates planning staffing levels to reflect arrival times of patients. An observational study of all patients who presented to the emergency department in July 2005 and February 2006 was undertaken (n=7768). The study identified 1577 patients suitable for treatment by the Advanced Nurse Practitioner (ANP) in these two months, which represents 20% of all patient attendances to the ED in this time period. A data collection tool was devised collectively by the ANPs to identify appropriate patients. The findings of the study revealed that 73% of patients suitable for the ANP service presented between the hours of 0800 and 2000, of which 54% attended between 0800 and 1600 h. Sunday emerged as the busiest day in July 2005 whereas Monday was found to be the busiest day in February 2006. Friday was found to be consistently busy for both months.

  4. Single-Molecule Studies of ssDNA-Binding Proteins Exchange.

    Science.gov (United States)

    Yang, Olivia; Ha, Taekjip

    2018-01-01

    Single-stranded DNA-binding protein (SSB) is important not only for the protection of single-stranded DNA (ssDNA) but also for the recruitment of other proteins for DNA replication, recombination, and repair. The interaction of SSB with ssDNA is highly dynamic as it exists as an intermediate during cellular processes that unwind dsDNA. It has been proposed that SSB redistributes itself among multiple ssDNA segments, but transient intermediates are difficult to observe in bulk experiments. We can use single-molecule FRET microscopy to observe intermediates of the transfer of a single Escherichia coli SSB from one ssDNA strand to another or exchange of one SSB for another on a single ssDNA in real time. This single-molecule approach can be further applicable to understand relative binding affinities and competitive dynamics for other SSBs and variants across various systems. © 2018 Elsevier Inc. All rights reserved.

  5. TIMES-SS - A mechanistic evaluation of an external validation study using reaction chemistry principles

    DEFF Research Database (Denmark)

    Roberts, David W.; Patlewicz, Grace; Dimitrov, Sabcho D.

    2007-01-01

    chemicals in the murine local lymph node assay (LLNA) and then compared with predictions made by TIMES-SS. The results were promising with an overall good concordance (83%) between experimental and predicted values. The LLNA results were evaluated with respect to reaction chemistry principles...... for sensitization. Additional testing on a further four chemicals was carried out to explore some of the specific reaction chemistry findings in more detail. Improvements for TIMES-SS, where appropriate, were put forward together with proposals for further research work. TIMES-SS is a promising tool to aid......The TImes MEtabolism Simulator platform used for predicting skin sensitization (TIMES-SS) is a hybrid expert system that was developed at Bourgas University using funding and data from a consortium comprised of industry and regulators. TIMES-SS encodes structure-toxicity and structure...

  6. Asymmetric PCR for good quality ssDNA generation towards DNA aptamer production

    Directory of Open Access Journals (Sweden)

    Junji Tominaga4

    2012-04-01

    Full Text Available Aptamers are ssDNA or RNA that binds to wide variety of target molecules with high affinity and specificity producedby systematic evolution of ligands by exponential enrichment (SELEX. Compared to RNA aptamer, DNA aptamer is muchmore stable, favourable to be used in many applications. The most critical step in DNA SELEX experiment is the conversion ofdsDNA to ssDNA. The purpose of this study was to develop an economic and efficient approach of generating ssDNA byusing asymmetric PCR. Our results showed that primer ratio (sense primer:antisense primer of 20:1 and sense primer amountof 10 to 100 pmol, up to 20 PCR cycles using 20 ng of initial template, in combination with polyacrylamide gel electrophoresis,were the optimal conditions for generating good quality and quantity of ssDNA. The generation of ssDNA via this approachcan greatly enhance the success rate of DNA aptamer generation.

  7. French validation of the Maslach Burnout Inventory-Student Survey (MBI-SS).

    Science.gov (United States)

    Faye-Dumanget, Christine; Carré, Julie; Le Borgne, Margaux; Boudoukha, Pr Abdel Halim

    2017-06-27

    Several international studies have been conducted on student burnout. To contribute to the clinical examination as well as research on the mental health of students, the MBI-SS (Maslach Burnout Inventory-Student Survey) has been validated and used in different countries but not in French. The aim of this study is to examine the validity of the 3-dimensional model of the French version of the MBI-SS, which is characterized by emotional "Emotional Exhaustion" (EE); "Cynicism" (CY); and low scores in "Academic Efficacy" (AE). A total of 667 university students were questioned to study the 3-dimensional structure of the French translation of the MBI-SS. The results validate the 3-dimensional structure of the MBI-SS and indicate satisfactory psychometric values. It is concluded that the MBI-SS can be used to assess burnout in French students. © 2017 John Wiley & Sons, Ltd.

  8. Selection and Application of ssDNA Aptamers against Clenbuterol Hydrochloride Based on ssDNA Library Immobilized SELEX.

    Science.gov (United States)

    Duan, Nuo; Gong, Wenhui; Wu, Shijia; Wang, Zhouping

    2017-03-01

    Clenbuterol hydrochloride (CLB) is often abused as additive feed for livestock to decrease adipose tissue deposition and to increase growth rate. It raises a potential risk to human health through the consumption of animal product. In this study, aptamers with higher affinity and specificity were screened through 16 selection rounds based on the ssDNA library immobilized systematic evolution of ligands by exponential enrichment (SELEX) technique. After cloning and sequencing, five aptamer candidates were picked out for affinity and specificity assays based on a graphene oxide (GO) adsorption method. The results showed that the aptamer CLB-2 binds specifically against CLB with a dissociation constant, Kd, value of 76.61 ± 12.70 nM. In addition, an aptamer-based fluorescence bioassay was established for CLB analysis. The correlation between the CLB concentration and fluorescent signal was found to be linear within the range of 0.10 to 50 ng/mL with a limit of detection of 0.07 ng/mL. It has been further applied for the determination of CLB in pork samples, showing its great potential for sensitive analysis in food safety control.

  9. Immunohistochemical study of KiSS1 and KiSS1R expression in human primary breast cancer: Association with breast cancer receptor status, proliferation markers and clinicopathological features.

    Science.gov (United States)

    Jarzabek, Katarzyna; Koda, Mariusz; Kozlowski, Leszek; Milewski, Robert; Wolczynski, Slawomir

    2015-06-01

    Recent studies have raised doubts about the protective role of KiSS1/KiSS1R in breast malignancy progression. However, the role of the KiSS1/KiSS1R system in primary breast cancer remains largely unknown. The aim of the present study was to characterize the biology and invasiveness potential of primary breast cancer through evaluation of KiSS1/KiSS1R protein expression and cellular localization with regard to lymph node metastasis status, receptor status (ERs, PR and HER-2/neu), and expression of aromatase, MMP-9, Ki-67 and Cyclin D1 in primary invasive breast cancer tissues. We showed increased protein expression of both KiSS1/KiSS1R and MMP-9 in the cancerous tissues compared with noncancerous tissue adjacent to the breast tumour. In the studied group of breast cancer samples, we observed a positive correlation between KiSS1 and MMP-9. We also showed a positive correlation between KiSS1R and aromatase expression in all studied breast cancers. We did not notice any associations between system and cell cycle regulators. KiSS1/KiSS1R did not correlate either with Cyclin D1 and Ki-67 or with receptor status. However, we showed higher levels of KiSS1R expression in ERα-negative cases than in ERα-positive cases in patients with lymph node metastasis. Present data do not confirm the protective role of KiSS1/KiSS1R in breast cancer progression, but our results do support the hypothesis that the KiSS1/KiSS1R system is activated even in primary breast cancer and sustained during invasion to local lymph nodes.

  10. Teaching Elementary School Social Studies Methods under edTPA

    Science.gov (United States)

    An, Sohyun

    2016-01-01

    This article reports a self-study that analyzes my experience as a teacher educator navigating a turbulent educational landscape with the advent of edTPA. The data consist of my journal entries, the syllabi, handouts, work submitted by my students, and course evaluations. Data were analyzed by using an inductive process to describe how the edTPA…

  11. AtlasT4SS: a curated database for type IV secretion systems.

    Science.gov (United States)

    Souza, Rangel C; del Rosario Quispe Saji, Guadalupe; Costa, Maiana O C; Netto, Diogo S; Lima, Nicholas C B; Klein, Cecília C; Vasconcelos, Ana Tereza R; Nicolás, Marisa F

    2012-08-09

    The type IV secretion system (T4SS) can be classified as a large family of macromolecule transporter systems, divided into three recognized sub-families, according to the well-known functions. The major sub-family is the conjugation system, which allows transfer of genetic material, such as a nucleoprotein, via cell contact among bacteria. Also, the conjugation system can transfer genetic material from bacteria to eukaryotic cells; such is the case with the T-DNA transfer of Agrobacterium tumefaciens to host plant cells. The system of effector protein transport constitutes the second sub-family, and the third one corresponds to the DNA uptake/release system. Genome analyses have revealed numerous T4SS in Bacteria and Archaea. The purpose of this work was to organize, classify, and integrate the T4SS data into a single database, called AtlasT4SS - the first public database devoted exclusively to this prokaryotic secretion system. The AtlasT4SS is a manual curated database that describes a large number of proteins related to the type IV secretion system reported so far in Gram-negative and Gram-positive bacteria, as well as in Archaea. The database was created using the RDBMS MySQL and the Catalyst Framework based in the Perl programming language and using the Model-View-Controller (MVC) design pattern for Web. The current version holds a comprehensive collection of 1,617 T4SS proteins from 58 Bacteria (49 Gram-negative and 9 Gram-Positive), one Archaea and 11 plasmids. By applying the bi-directional best hit (BBH) relationship in pairwise genome comparison, it was possible to obtain a core set of 134 clusters of orthologous genes encoding T4SS proteins. In our database we present one way of classifying orthologous groups of T4SSs in a hierarchical classification scheme with three levels. The first level comprises four classes that are based on the organization of genetic determinants, shared homologies, and evolutionary relationships: (i) F-T4SS, (ii) P-T4SS, (iii

  12. AtlasT4SS: A curated database for type IV secretion systems

    Directory of Open Access Journals (Sweden)

    Souza Rangel C

    2012-08-01

    Full Text Available Abstract Background The type IV secretion system (T4SS can be classified as a large family of macromolecule transporter systems, divided into three recognized sub-families, according to the well-known functions. The major sub-family is the conjugation system, which allows transfer of genetic material, such as a nucleoprotein, via cell contact among bacteria. Also, the conjugation system can transfer genetic material from bacteria to eukaryotic cells; such is the case with the T-DNA transfer of Agrobacterium tumefaciens to host plant cells. The system of effector protein transport constitutes the second sub-family, and the third one corresponds to the DNA uptake/release system. Genome analyses have revealed numerous T4SS in Bacteria and Archaea. The purpose of this work was to organize, classify, and integrate the T4SS data into a single database, called AtlasT4SS - the first public database devoted exclusively to this prokaryotic secretion system. Description The AtlasT4SS is a manual curated database that describes a large number of proteins related to the type IV secretion system reported so far in Gram-negative and Gram-positive bacteria, as well as in Archaea. The database was created using the RDBMS MySQL and the Catalyst Framework based in the Perl programming language and using the Model-View-Controller (MVC design pattern for Web. The current version holds a comprehensive collection of 1,617 T4SS proteins from 58 Bacteria (49 Gram-negative and 9 Gram-Positive, one Archaea and 11 plasmids. By applying the bi-directional best hit (BBH relationship in pairwise genome comparison, it was possible to obtain a core set of 134 clusters of orthologous genes encoding T4SS proteins. Conclusions In our database we present one way of classifying orthologous groups of T4SSs in a hierarchical classification scheme with three levels. The first level comprises four classes that are based on the organization of genetic determinants, shared homologies, and

  13. TechEdSat Nano-Satellite Series Fact Sheet

    Science.gov (United States)

    Murbach, Marcus; Martinez, Andres; Guarneros Luna, Ali

    2014-01-01

    TechEdSat-3p is the second generation in the TechEdSat-X series. The TechEdSat Series uses the CubeSat standards established by the California Polytechnic State University Cal Poly), San Luis Obispo. With typical blocks being constructed from 1-unit (1U 10x10x10 cm) increments, the TechEdSat-3p has a 3U volume with a 30 cm length. The project uniquely pairs advanced university students with NASA researchers in a rapid design-to-flight experience lasting 1-2 semesters.The TechEdSat Nano-Satellite Series provides a rapid platform for testing technologies for future NASA Earth and planetary missions, as well as providing students with an early exposure to flight hardware development and management.

  14. ED leadership competency matrix: an administrative management tool.

    Science.gov (United States)

    Propp, Douglas A; Glickman, Seth; Uehara, Dennis T

    2003-10-01

    A successful ED relies on its leaders to master and demonstrate core competencies to be effective in the many arenas in which they interact and are responsible. A unique matrix model for the assessment of an ED leadership's key administrative skill sets is presented. The model incorporates capabilities related to the individual's cognitive aptitude, experience, acquired technical skills, behavioral characteristics, as well as the ability to manage relationships effectively. Based on the personnel inventory using the matrix, focused evaluation, development, and recruitment of ED key leaders occurs. This dynamic tool has provided a unique perspective for the evaluation and enhancement of overall ED leadership performance. It is hoped that incorporation of such a model will similarly improve the accomplishments of EDs at other institutions.

  15. A novel protein elicitor (SsCut) from Sclerotinia sclerotiorum induces multiple defense responses in plants.

    Science.gov (United States)

    Zhang, Huajian; Wu, Qun; Cao, Shun; Zhao, Tongyao; Chen, Ling; Zhuang, Peitong; Zhou, Xiuhong; Gao, Zhimou

    2014-11-01

    In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco that closely resembled hypersensitive response lesions. Mutational analysis identified the C-terminal-half peptide and the same amino acids indispensable for both enzyme and elicitor activity. His-SsCut was caused cell death in Arabidopsis, soybean (Glycine max), oilseed rape (Brassica napus), rice (Oryza sativa), maize (Zea mays), and wheat (Triticum aestivum), indicating that both dicot and monocot species are responsive to the elicitor. Furthermore, the elicitation of tobacco was effective in the induction of the activities of hydrogen peroxide, phenylalanine ammonia-lyase, peroxides, and polyphenol oxidase. His-SsCut-treated plants exhibited enhanced resistance as indicated by a significant reduction in the number and size of S. sclerotiorum, Phytophthora sojae, and P. nicotianae lesions on leaves relative to controls. Real-time PCR results indicated that the expression of defense-related genes and genes involved in signal transduction were induced by His-SsCut. Our results demonstrate that SsCut is an elicitor that triggers defense responses in plants and will help to clarify its relationship to downstream signaling pathways that induce defense responses.

  16. Graphene oxide modified light addressable potentiometric sensor and its application for ssDNA monitoring.

    Science.gov (United States)

    Jia, Yunfang; Yin, Xue-Bo; Zhang, Jia; Zhou, Shuang; Song, Meng; Xing, Ke-Li

    2012-12-21

    A light addressable potentiometric sensor (LAPS) is a kind of silicon based semiconductor sensor, and surface modification is a fundamental problem for its application in biological fields. Graphene oxide (GO) based biochemically activated LAPS were proposed, called GO-LAPS. The GO-LAPS were applied to monitoring single strand DNA (ssDNA) probe immobilization and its hybridization with complementary ssDNA molecules of different chain lengths (30, 21 and 14 base pairs, respectively). It was discovered that the curves of LAPS' currents versus analyte concentrations for ssDNA probe binding and the target ssDNA hybridization were different. Explanations were proposed based on the semiconductor's surface-electric-field-effect and the electrical properties of ssDNA molecule. Moreover, comparisons between GO-LAPS and LAPS without GO modification were carried out. Enhanced response currents of GO-LAPS were reported experimentally and analyzed theoretically based on X-ray photoelectron spectroscopy (XPS) of GO-LAPS. The limitation of target ssDNA monitoring was 1 pM to 10 nM, which suggested that this LAPS based platform could be developed as a sensitive means for short chain ssDNA detection.

  17. Using Kentucky State Standards as Benchmarks: Quantifying Incoming Ed.S. Students' Knowledge as They Journey toward Principalship

    Science.gov (United States)

    Hearn, Jessica E.

    2015-01-01

    Principal preparation programs in Kentucky can use the items in the Dispositions, Dimensions, and Functions for School Leaders (EPSB, 2008) as mastery benchmarks to quantify incoming Educational Specialist (Ed.S) students' perceived level of mastery. This can serve both internal and external purposes by providing diagnostic feedback to students…

  18. Construction, characterization and evaluation of the protective efficacy of the Streptococcus suis double mutant strain ΔSsPep/ΔSsPspC as a live vaccine candidate in mice.

    Science.gov (United States)

    Hu, Jin; You, Wujin; Wang, Bin; Hu, Xueying; Tan, Chen; Liu, Jinlin; Chen, Huanchun; Bei, Weicheng

    2015-01-01

    Streptococcus suis serotype 2 (S. suis 2) causes sepsis and meningitis in piglets and humans, and results in one of the most serious bacterial diseases affecting the production of commercial pigs around the world. Due to the failure of the current inactivated vaccine to protect against the disease, development of a new attenuated live vaccine against S. suis 2 by deleting essential virulence factors is urgently needed. We have previously reported the construction and characterization of an SsPep single gene deletion mutant strain ΔSsPep based on S. suis 2. Our previous results have shown that SsPep plays a critical role in the pathogenesis of S. suis 2. In this study, a precisely defined double-deletion mutant ΔSsPep/ΔSsPspC of S. suis 2 without antibiotic-resistance markers was constructed based on ΔSsPep, and the levels of virulence of the wild-type (WT) and ΔSsPep/ΔSsPspC were compared in a mouse experimental infection model. We demonstrated that the double mutant ΔSsPep/ΔSsPspC was less virulent than the WT, and could induce a noticeable antibody response. Analysis of IgG subclasses (IgG1 and IgG2a) indicated that both Th1 and Th2 responses were induced by ΔSsPep/ΔSsPspC, although the IgG2a (Th1) response predominated over the IgG1 (Th2) response. Moreover, ΔSsPep/ΔSsPspC could confer 90% protective efficacy against challenge with a lethal dose of fully virulent S. suis 2. Taken together, these data demonstrate that ΔSsPep/ΔSsPspC can be used as an effective live vaccine and provide a novel strategy against infection of S. suis 2. Copyright © 2014 Elsevier GmbH. All rights reserved.

  19. Corrosion Resistance of 304L SS Spray Coated with Zirconia Nanoparticles

    Science.gov (United States)

    Maheswari, A. Uma; Sivakumar, M.; Indhumathi, N.; Mohan, Sreedevi R.

    2016-09-01

    Influence of substrate temperature on corrosion (in 3.5% NaCl) and wear resistance of nanostructured zirconia thin film coated 304L SS substrates are studied by electrochemical and nano-indentation methods. This analysis shows 304L SS substrate spray coated with nanostructured zirconia at substrate temperature closer to the boiling point of the spray solvent ethanol exhibited good corrosion and wear resistance behaviour. This is because of the compressive stress developed during film fabrication at lower substrate temperature (∼50 °C) and hence constrains the indentation plasticity, which leads to higher indentation load than the bare 304L SS.

  20. Modulación de la expresión por GA y ABA de los genes Ss1 y Ss2 que codifican sacarosa sintasa en cebada

    OpenAIRE

    Carbonero Zalduegui, Pilar; Barrero Sicilia, Cristina; Oñate Sanchez, Luis; Hernando Amado, Sara; Rueda Romero, Paloma

    2008-01-01

    En este trabajo se ha llevado a cabo un estudio comparativo entre distintas isoformas de SUSy de cereales y arabidopsis. Además se ha realizado un análisis de expresión de HvSs1 y HvSs2 en distintos órganos, incluyendo patrones temporales en semillas en desarrollo y germinación, así como la variación de su respuesta a ácido abscísico (ABA) y giberélico (GA3).

  1. The Synovial Sarcoma-Associated SS18-SSX2 Fusion Protein Induces Epigenetic Gene (De)Regulation.

    NARCIS (Netherlands)

    Bruijn, D.R.H. de; Allander, S.V.; Dijk, A.H.A.; Willemse, M.P.; Thijssen, J.; Groningen, J.J.M. van; Meltzer, P.S.; Geurts van Kessel, A.H.M.

    2006-01-01

    Fusion of the SS18 and either one of the SSX genes is a hallmark of human synovial sarcoma. The SS18 and SSX genes encode nuclear proteins that exhibit opposite transcriptional activities. The SS18 protein functions as a transcriptional coactivator and is associated with the SWI/SNF complex, whereas

  2. The kisspeptin (KiSS-1)/GPR54 system in cancer biology.

    Science.gov (United States)

    Makri, Angeliki; Pissimissis, Nikolaos; Lembessis, Peter; Polychronakos, Constantine; Koutsilieris, Michael

    2008-12-01

    Kisspeptin (KiSS-1) gene, initially described as a melanoma metastasis suppressor gene, encodes a number of peptides (kp-54, kp-14, kp-13, kp-10), which are endogenous ligands to a G protein-coupled receptor, referred as hOT7T175 or AXOR12 or GPR54. So far intensive investigation has provided substantiate evidence supporting the role of KiSS-1/GPR54 system in cancer biology as well as in the regulation of the reproductive function and trophoblast invasion. The precise mechanism by which KiSS-1/GPR54 system is affecting cancer cell growth and metastasis includes complex endocrine, paracrine and autocrine actions. Nevertheless, the detail mechanism of such actions is still under intensive investigation. Herein we review the evidence which support the role of KiSS-1/GPR54 system in cancer biology.

  3. Naisten ja miesten eriarvoisuus työelämässä Suomessa

    OpenAIRE

    Aho, Emilia

    2014-01-01

    Työn tavoitteena on tutkia syitä naisten ja miesten eriarvoiseen asemaan työelämässä. Työssä keskitytään etenkin palkkaeroihin, joista naisten ja miesten eriarvoisuus työelämässä on nähtävissä selvästi. Jotta tasa-arvoisuuteen päästäisiin naisten ja miesten välillä, on pyrittävä tukemaan heikommassa asemassa olevia. Työnantajan tulee aktiivisesti edistää tasa-arvoa työelämässä, ja tasa-arvosuunnitelman laatiminen on yksi askel kohti tasa-arvoisempaa työyhteisöä. Lainsäädäntö asettaa vaat...

  4. Grüss type integral inequalities for generalized Riemann-Liouville k-fractional integrals

    National Research Council Canada - National Science Library

    Mubeen, Shahid; Iqbal, Sana

    2016-01-01

    .... In this research article, we present the improved version of generalizations for a Grüss type integral inequality by taking a generalized Riemann-Liouville fractional integral in terms of a new parameter k > 0 $k>0...

  5. Bee Wax Propolis Extract as Eco-Friendly Corrosion Inhibitors for 304SS in Sulfuric Acid

    Directory of Open Access Journals (Sweden)

    Femiana Gapsari

    2015-01-01

    Full Text Available The inhibition properties of bee wax propolis (BWP extract on the 304SS in 0.5 M sulfuric acid were conducted using potentiodynamic polarization, EIS, and XRD. Quercetin (2-(3.4-dihydroxy phenyl-3.5.7-trihydroxy-4H-chromen-4-one was identified as the main compound in the BWP extract based on FTIR and HPLC analysis. The results showed that the inhibitor could retard the corrosion rate of 304SS in 0.5 M sulfuric acid which reached 97.29% and 91.42% at 2000 ppm based on potentiodynamic polarization and EIS measurement, respectively. The inhibition efficiency decreased with increasing temperature. The inhibition mechanism of BWP extract on the 304SS was physisorption and obeyed Temkin adsorption isotherm equation. The thin protective layer on the 304SS surface was confirmed by XRD.

  6. The Blattodea s.s. (Insecta, Dictyoptera) of the Guiana Shield

    National Research Council Canada - National Science Library

    Evangelista, Dominic A; Chan, Kimberly; Kaplan, Kayla L; Wilson, Megan M; Ware, Jessica L

    2015-01-01

    .... The complete checklist contains 234 species of Blattodea s.s. currently known in the shield. This checklist shows particularly low richness in Guianan Venezuela, Roraima and Amapa Brazil, but this is likely an artifact due to under–sampling...

  7. An investigation of p-normed sparsity evaluation for SS-LBP-based edge extraction

    Energy Technology Data Exchange (ETDEWEB)

    Chen-yi, Zhao [School of Physics, Northeast Normal University, Changchun (China); Jia-ning, Sun, E-mail: sunjn118@nenu.edu.cn [School of Mathematics and Statistics, Northeast Normal University, Changchun (China); Shuang, Qiao, E-mail: qiaos810@nenu.edu.cn [School of Physics, Northeast Normal University, Changchun (China)

    2017-02-01

    SS-LBP is a very efficient tool for fast edge extraction in digital radiography. In this paper, we introduce a p-normed sparsity evaluation strategy to improve and generalize the existing SS-LBP framework. To illustrate the feasibility of the proposed approach, several experimental results are presented. Comparisons show that 1-normed sparsity evaluation is more effective and robust in practical applications.

  8. lsnjbhakta@gmail.com A Comparative SEM-EDS Elemental ...

    African Journals Online (AJOL)

    Michael Horsfall

    X25) and microscopic. (X5000) pictures of the mud exhibited a wide range of shapes and surface texture for individual particle. Elemental criteria: EDS analysis showed the clear distribution criteria of ten elements, Carbon,. Phosphorous,. Sulfur ...

  9. Erectile Dysfunction Herbs: A Natural Treatment for ED?

    Science.gov (United States)

    ... erectile dysfunction: A natural treatment for ED? Some herbs claim to help erectile dysfunction. Find out the ... You've likely seen advertisements for erectile dysfunction herbs or supplements to "increase your sexual performance." Could ...

  10. Review: Gravers, Mikael (ed. (2007, Exploring Ethnic Diversity in Burma

    Directory of Open Access Journals (Sweden)

    Roland Mischung

    2009-01-01

    Full Text Available Review of the edited volume: Gravers, Mikael (ed., Exploring Ethnic Diversity in Burma, Copenhagen: NIAS Press (= NIAS Studies in Asian Topics Series, 39, 2007, ISBN 9788791114960, 283 pages

  11. Elementi ed esercizi di geometria analitica e proiettiva

    CERN Document Server

    Francia, Giovanni

    1950-01-01

    La retta : preliminario ; coordinate cartesiane nel piano, equazioni della retta, distanze ed angoli ; cambiamento delle coordinate, coordinate polari ; il cerchio ; le coniche come luoghi geometrici : l'ellisse, l'iperbole, parabola.

  12. Structure of catalase determined by MicroED

    Science.gov (United States)

    Nannenga, Brent L; Shi, Dan; Hattne, Johan; Reyes, Francis E; Gonen, Tamir

    2014-01-01

    MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of still diffraction patterns to determine the structure of lysozyme at 2.9 Å resolution with MicroED (Shi et al., 2013). Here we present the structure of bovine liver catalase determined from a single crystal at 3.2 Å resolution by MicroED. The data were collected by continuous rotation of the sample under constant exposure and were processed and refined using standard programs for X-ray crystallography. The ability of MicroED to determine the structure of bovine liver catalase, a protein that has long resisted atomic analysis by traditional electron crystallography, demonstrates the potential of this method for structure determination. DOI: http://dx.doi.org/10.7554/eLife.03600.001 PMID:25303172

  13. Nuclear Medicine Physics: The Basics. 7th ed.

    Science.gov (United States)

    Mihailidis, Dimitris

    2012-10-01

    Nuclear Medicine Physics: The Basics. 7th ed. Ramesh Chandra, Lippincott Williams and Wilkins, a Wolters Kluwer Business. Philadelphia, 2012. Softbound, 224 pp. Price: $69.99. ISBN: 9781451109412. © 2012 American Association of Physicists in Medicine.

  14. Promotion of plant growth by Pseudomonas fluorescens strain SS101 via novel volatile organic compounds.

    Science.gov (United States)

    Park, Yong-Soon; Dutta, Swarnalee; Ann, Mina; Raaijmakers, Jos M; Park, Kyungseok

    2015-05-29

    Volatile organic compounds (VOCs) from plant growth-promoting rhizobacteria (PGPR) play key roles in modulating plant growth and induced systemic resistance (ISR) to pathogens. Despite their significance, the physiological functions of the specific VOCs produced by Pseudomonas fluorescens SS101 (Pf.SS101) have not been precisely elucidated. The effects of Pf.SS101 and its VOCs on augmentation of plant growth promotion were investigated in vitro and in planta. A significant growth promotion was observed in plants exposed Pf.SS101 under both conditions, suggesting that its VOCs play a key role in promoting plant growth. Solid-phase micro-extraction (SPME) and a gas chromatography-mass spectrophotometer (GC-MS) system were used to characterize the VOCs emitted by Pf.SS101 and 11 different compounds were detected in samples inoculated this bacterium, including 13-Tetradecadien-1-ol, 2-butanone and 2-Methyl-n-1-tridecene. Application of these compounds resulted in enhanced plant growth. This study suggests that Pf.SS101 promotes the growth of plants via the release of VOCs including 13-Tetradecadien-1-ol, 2-butanone and 2-Methyl-n-1-tridecene, thus increasing understanding of the role of VOCs in plant-bacterial inter-communication. Copyright © 2015 Elsevier Inc. All rights reserved.

  15. KiSS1 and its G-protein-coupled receptor GPR54 in cancer development and metastasis.

    Science.gov (United States)

    Cho, Sung-Gook; Li, Dali; Tan, Kunrong; Siwko, Stefan K; Liu, Mingyao

    2012-12-01

    KiSS1 and its cognate G-protein-coupled receptor, GPR54, have diverse functions. While KiSS1 and GPR54 have been intensively studied in physiology, their role in cancer is still unclear. In cancer, KiSS1 and GPR54 have been known to suppress metastasis by inhibiting cancer cell motility. However, recent studies suggest that KiSS1 and GPR54 have varied roles even in cancer development and metastasis. Here, we examine recent advances in understanding the roles of KiSS1 and GPR54 in cancer development and metastasis.

  16. Validation of the Stroke Specific Quality of Life Scale (SS-QOL): test of reliability and validity of the Danish version (SS-QOL-DK).

    Science.gov (United States)

    Muus, Ingrid; Williams, Linda S; Ringsberg, Karin C

    2007-07-01

    To test the reliability and validity of the Danish version of the Stroke Specific Quality of Life Scale version 2.0 (SS-QOL-DK), an instrument for evaluation of health-related quality of life. A correlational study. A stroke unit that provides acute care and rehabilitation for stroke patients in Frederiksborg County, Denmark. One hundred and fifty-two stroke survivors participated; 24 of these performed test-retest. Questionnaires were sent out and returned by mail. A subsequent telephone interview assessed functional level and missing items. Test-retest was measured using Spearman's r, internal consistency was estimated using Cronbach's alpha, and evaluation of floor and ceiling values in proportion of minimum and maximum scores. Construct validity was assessed by comparing patients' scores on the SS-QOL-DK with those obtained by other test methods: Beck's Depression Index, the General Health Survey Short Form 36 (SF-36), the Barthel Index and the National Institutes of Health Stroke Scale, evaluating shared variance using coefficient of determination, r2. Comparing groups with known scores assessed known-group validity. Convergent and discriminant validity were assessed. Test-retest of SS-QOL-DK showed excellent stability, Spearman's r = 0.65-0.99. Internal consistency for all domains showed Cronbach's alpha = 0.81-0.94. Missing items rate was 1.0%. Most SS-QOL-DK domains showed moderately shared variance with similar domains of other test methods, r2 = 0.03-0.62. Groups with known differences showed statistically significant difference in scores. Item-to-scale correlation coefficients of 0.37-0.88 supported convergent validity. SS-QOL-DK is a reliable and valid instrument for measuring self-reported health-related quality of life on group level among people with mild to moderate stroke.

  17. Ambienti Circumstellari ed Interstellari di Supernovae di vario Tipo ed Applicazioni Astrofisiche

    Science.gov (United States)

    Boffi, Francesca R.

    Nella presente tesi vengono studiati gli ambienti circumstellari ed interstellari di supernovae (anche SNe; singolare SN) di vario tipo. In particolare si descrivono alcune applicazioni astrofisiche, relative a questi ambienti, che permettono di desumere informazioni sui sistemi stellari progenitori delle supernovae o di determinare le distanze a tali oggetti. L' emissione radio da supernovae, prodotta nell' ambiente circumstellare ad opera dell'interazione idrodinamica del gas di SN con l' ambiente circostante, puo' essere impiegata come mezzo di indagine del sistema di pre-supernova. In particolare si e' introdotta l'idea che tale emissione possa essere impiegata nel caso delle SNe di tipo Ia come test dei sistemi progenitori di tipo simbiotico. Il test e' stato effettuato sulla SN 1986G, per la quale avevamo ottenuto limiti superiori a lunghezze d'onda radio. Nel caso che un sistema simbiotico sia responsabile di una SN Ia, l'interazione tra l' ejecta e una shell circumstellare origina, in un'epoca molto prossima al massimo di luce, un'emissione radio rivelabile con strumenti tipo Very Large Array. Inoltre l'emissione radio fornisce informazioni circa gli stadi di pre-supernova nel caso di altri tipi di supernova. In questo lavoro di tesi vengono presentati i risultati ottenuti nel caso delle SNe di tipo II 1984E e 1986E. Queste due SNe non mostrano emissione radio: la prima, in prossimita del massimo ottico, stava interagendo con una shell circumstellare prodotta da un episodio di perdita di massa di breve durata in fase di pre-supernova; la seconda, invece e' la prima SN ``vecchia'' ad essere rivelata nell' ottico ma non nel radio (l' oggetto non ha ancora iniziato la fase di supernova remnant ed emette radiazione ottica prodotta ancora per interazione circumstellare). In ambiente circum-interstellare, gli echi di luce, prodotti dallo scattering della luce della supernova ad opera della polvere presente, possono essere utilizzati per determinare le distanze

  18. Validity of the SS-QOL in Germany and in survivors of hemorrhagic or ischemic stroke.

    Science.gov (United States)

    Ewert, Thomas; Stucki, Gerold

    2007-01-01

    The Stroke-Specific Quality of Life Scale (SS-QOL) is a recently developed measure to assess health-related quality of life in stroke patients. The objective of this study was to translate the American version of the SS-QOL and examine the validity of the German proxy version, in both ischemic and hemorrhagic stroke survivors. The translation was conducted according to published guidelines. The validation was performed in consecutive adult stroke survivors. Data were obtained 1 year after discharge. To examine the dimensionality of the SS-QOL, factor analyses were conducted. The validity was examined by the associations of the subscales with the Functional Independence Measure and Short Form 36. The literal translation revealed no major changes between the American and the German versions of the SS-QOL. Three hundred seven stroke survivors were included in the study. Unlike the 1st validation study, most of the variance could be explained by 8 instead of 12 factors; therefore, the 8-factor solution was further examined. The validity of the SS-QOL total score and "observable" scales such as "activities" was shown. For the German proxy version of the SS-QOL, an 8-factor solution was found to be the most appropriate. The psychometric properties of these 8 subscales were good or excellent with respect to internal consistency. The validity of the total score was shown, but some subscales (energy, mood, and thinking) failed the hypothesized associations. Therefore, the SS-QOL needs to be further explored in other settings and populations.

  19. Metabolic and transcriptomic changes induced in Arabidopsis by the rhizobacterium Pseudomonas fluorescens SS101.

    Science.gov (United States)

    van de Mortel, Judith E; de Vos, Ric C H; Dekkers, Ester; Pineda, Ana; Guillod, Leandre; Bouwmeester, Klaas; van Loon, Joop J A; Dicke, Marcel; Raaijmakers, Jos M

    2012-12-01

    Systemic resistance induced in plants by nonpathogenic rhizobacteria is typically effective against multiple pathogens. Here, we show that root-colonizing Pseudomonas fluorescens strain SS101 (Pf.SS101) enhanced resistance in Arabidopsis (Arabidopsis thaliana) against several bacterial pathogens, including Pseudomonas syringae pv tomato (Pst) and the insect pest Spodoptera exigua. Transcriptomic analysis and bioassays with specific Arabidopsis mutants revealed that, unlike many other rhizobacteria, the Pf.SS101-induced resistance response to Pst is dependent on salicylic acid signaling and not on jasmonic acid and ethylene signaling. Genome-wide transcriptomic and untargeted metabolomic analyses showed that in roots and leaves of Arabidopsis plants treated with Pf.SS101, approximately 1,910 genes and 50 metabolites were differentially regulated relative to untreated plants. Integration of both sets of "omics" data pointed to a prominent role of camalexin and glucosinolates in the Pf.SS101-induced resistance response. Subsequent bioassays with seven Arabidopsis mutants (myb51, cyp79B2cyp79B3, cyp81F2, pen2, cyp71A12, cyp71A13, and myb28myb29) disrupted in the biosynthesis pathways for these plant secondary metabolites showed that camalexin and glucosinolates are indeed required for the induction of Pst resistance by Pf.SS101. Also for the insect S. exigua, the indolic glucosinolates appeared to play a role in the Pf.SS101-induced resistance response. This study provides, to our knowledge for the first time, insight into the substantial biochemical and temporal transcriptional changes in Arabidopsis associated with the salicylic acid-dependent resistance response induced by specific rhizobacteria.

  20. Patient anxiety may influence the efficacy of ED pain management.

    Science.gov (United States)

    Craven, Philip; Cinar, Orhan; Madsen, Troy

    2013-02-01

    The aim of this study was to evaluate the incidence of anxiety and rates of anxiety treatment in emergency department (ED) patients presenting with pain-related complaints. We prospectively evaluated patients in an urban academic tertiary care hospital ED from 2000 through 2010. We enrolled a convenience sample of adult patients presenting with pain and recorded patient complaint, medication administration, satisfaction, and pain and anxiety scores throughout their stay. We stratified patients into 4 different groups according to anxiety score at presentation (0, none; 1-4, mild; 5-7, moderate; 8-10, severe). We enrolled 10 664 ED patients presenting with pain-related complaints. Patients reporting anxiety were as follows: 25.7%, none; 26.1%, mild; 23.7%, moderate; and 24.5%, severe. Although 48% of patients described moderate to severe anxiety at ED presentation and 60% were willing to take a medication for anxiety, only 1% received anxiety treatment. Thirty-five percent of patients still reported moderate/severe anxiety at discharge. Severe anxiety at ED presentation was associated with increased demand for pain medication (odds ratio [OR], 1.40; 95% confidence interval [CI], 1.10-1.79) and anxiety medication (OR, 4.34; 95% CI, 3.68-5.11) during the ED stay and decreased satisfaction with the treatment of pain (β coefficient = -0.328; P < .001). After adjusting for age, sex, and presentation pain scores, patients who reported severe anxiety were more likely to receive an analgesic (OR, 1.33; 95% CI, 1.19-1.50) and an opioid (OR, 1.25; 95% CI, 1.11-1.41) during the ED stay. Anxiety may be underrecognized and undertreated in patients presenting with pain-related complaints. Patients reporting severe anxiety were less likely to report satisfaction with the treatment of their pain, despite higher rates of analgesic administration. Copyright © 2013 Elsevier Inc. All rights reserved.

  1. MANY MEN USED ED TREATMENT WITHOUT THEIR PARTNERS’ KNOWLEDGE

    Directory of Open Access Journals (Sweden)

    Tan HM

    2007-01-01

    Full Text Available This paper aimed to identify and explore the understanding and perception of erectile dysfunction (ED using exploratory qualitative approaches. This study was conducted in Klang Valley, Malaysia. Purposive sampling was used to recruit 17 urban men aged 40-75 years. A semi-structural interview guide was used to explore men’s understanding of ED, impacts on their well-being and experiences with treatments. The focus groups revealed that ED was perceived to be an important loss in men’s lives as sexual performance was closely related to manhood. The men associated ED with a loss of masculinity and self-esteem. Men’s understanding of ED treatments was restricted to phophodiesterase type 5 inhibitors (PDE-5 inhibitors and traditional therapies. Although PDE-5 inhibitors were perceived to be effective, they were concerned about their safety and costs. Some of the men have learned to cope with ED rather than to seek help, particularly among older men, who attributed their decreased sexual capacities and erection difficulties to aging, and therefore conditioned themselves to accepting it. In contrast, younger men who have erectile difficulties are considered as ‘abnormal’, and are more likely to seek help and receive treatment for their sexual problem. Men’s knowledge of and experience with modern treatments of ED were mainly associated with PDE-5 inhibitors. Their perception towards PDE-5 inhibitors was largely influenced by personal experience, information from mass media and friends. The high cost and fear of side effects were two main deterrents in the use of PDE-5 inhibitors. Many men had conceded their use of PDE-5 inhibitors without their partners’ knowledge. They worried that their partners would view the matter with suspicion and mistrust.

  2. A visual assay to monitor T6SS-mediated bacterial competition.

    Science.gov (United States)

    Hachani, Abderrahman; Lossi, Nadine S; Filloux, Alain

    2013-03-20

    Type VI secretion systems (T6SSs) are molecular nanomachines allowing Gram-negative bacteria to transport and inject proteins into a wide variety of target cells(1,2). The T6SS is composed of 13 core components and displays structural similarities with the tail-tube of bacteriophages(3). The phage uses a tube and a puncturing device to penetrate the cell envelope of target bacteria and inject DNA. It is proposed that the T6SS is an inverted bacteriophage device creating a specific path in the bacterial cell envelope to drive effectors and toxins to the surface. The process could be taken further and the T6SS device could perforate other cells with which the bacterium is in contact, thus injecting the effectors into these targets. The tail tube and puncturing device parts of the T6SS are made with Hcp and VgrG proteins, respectively(4,5). The versatility of the T6SS has been demonstrated through studies using various bacterial pathogens. The Vibrio cholerae T6SS can remodel the cytoskeleton of eukaryotic host cells by injecting an "evolved" VgrG carrying a C-terminal actin cross-linking domain(6,7). Another striking example was recently documented using Pseudomonas aeruginosa which is able to target and kill bacteria in a T6SS-dependent manner, therefore promoting the establishment of bacteria in specific microbial niches and competitive environment(8,9,10). In the latter case, three T6SS-secreted proteins, namely Tse1, Tse2 and Tse3 have been identified as the toxins injected in the target bacteria (Figure 1). The donor cell is protected from the deleterious effect of these effectors via an anti-toxin mechanism, mediated by the Tsi1, Tsi2 and Tsi3 immunity proteins(8,9,10). This antimicrobial activity can be monitored when T6SS-proficient bacteria are co-cultivated on solid surfaces in competition with other bacterial species or with T6SS-inactive bacteria of the same species(8,11,12,13). The data available emphasized a numerical approach to the bacterial

  3. Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants

    Directory of Open Access Journals (Sweden)

    Renata Fava Ditt

    2011-08-01

    Full Text Available Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp. is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC. With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify sugarcane proteins that interact with SsNAC23. Using SsNAC23 as bait, we screened a cDNA expression library of sugarcane plants submitted to 4ºC for 48 h. Several interacting partners were identified, including stress-related proteins, increasing our knowledge on how sugarcane plants respond to cold stress. One of these interacting partners, a thioredoxin h1, offers insights into the regulation of SsNAC23 activity.

  4. Differential Role of the T6SS in Acinetobacter baumannii Virulence

    Science.gov (United States)

    Foucault-Grunenwald, Marie-Laure; Borges, Vitor; Charpentier, Xavier; Limansky, Adriana S.; Gomes, João Paulo; Viale, Alejandro M.; Salcedo, Suzana P.

    2015-01-01

    Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR) strains. Herein, we compared a type strain (ATCC17978), a non-clinical isolate (DSM30011) and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825), revealing distinct patterns of type VI secretion system (T6SS) functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner. PMID:26401654

  5. Fluorescence Detection and Discrimination of ss- and ds-DNA with a Water Soluble Oligopyrene Derivative

    Directory of Open Access Journals (Sweden)

    Gaoquan Shi

    2009-06-01

    Full Text Available A novel water-soluble cationic conjugated oligopyrene derivative, oligo(N1,N1,N1,N4,N4,N4-hexamethyl-2-(4-(pyren-1-yl butane-1,4-diaminium bromide (OHPBDB, was synthesized by a combination of chemical and electrochemical synthesis techniques. Each oligomer chain has five pyrene derivative repeating units and brings 10 positive charges. OHPBDB showed high and rapid fluorescence quenching in aqueous media upon addition of trace amounts of single-stranded (ss and double-stranded (ds DNA. The Stern-Volmer constants for ss- and ds-DNA were measured to be as high as 1.3 × 108 mol-1·L and 1.2 × 108 mol-1·L, respectively. On the other hand, distinct fluorescence enhancement of OHPBDB upon addition of large amount of ss-DNA or ds-DNA was observed. Furthermore, ss-DNA showed much stronger fluorescence enhancement than that of ds-DNA, thus yielding a clear and simple signal useful for the discrimination between ss- and ds-DNA in aqueous media.

  6. Study on the IFNL4 gene ss469415590 variant in Ukrainian population

    Directory of Open Access Journals (Sweden)

    Kucherenko A. M.

    2014-09-01

    Full Text Available Aim. To determine genotype and allele disribution for the IFNL4 gene ss469415590 and examine it for linkage with the IL28B gene rs12979860 in Ukrainian population. Methods. The studied group consisted of 100 unrelated donors of Eastern European origin representing the population of Ukraine. Genotyping for the IFNL4 gene ss469415590 was performed using the amplification-refractory mutation system PCR. Genotyping for the IL28B gene rs12979860 was performed by the PCR-based restriction fragment length polymorphism assay. Results. Genotype frequencies for both studied variants showed no significant deviation from those expected according to Hardy-Weinberg equilibrium. Allelic distribution for ss469415590 was: TT – 0.665, G – 0.335. Allelic frequencies of rs12979860 were: C – 0.655, T – 0.345. The results of likelihood ratio test indicated a linkage disequilibrium between the studied variants (p > 0.0001, the major alleles ss469415590 TT and rs12979860 C were in phase. The genetic structure of Ukrainian population in terms of two studied polymorphic variants is similar to the European population presented in the «1000 genomes» project. Conclusions. Considering a tight linkage revealed in Ukrainian population between the ss469415590 variant and rs12979860, a crucial genetic marker of chronic hepatitis C treatment efficiency, this polymorphism might be a promising target for further investigation as a pharmacogenetic marker.

  7. Lead (Pb) bioaccumulation; genera Bacillus isolate S1 and SS19 as a case study

    Science.gov (United States)

    Arifiyanto, Achmad; Apriyanti, Fitria Dwi; Purwaningsih, Puput; Kalqutny, Septian Hary; Agustina, Dyah; Surtiningsih, Tini; Shovitri, Maya; Zulaika, Enny

    2017-06-01

    Lead (Pb) includes a group of large heavy metal in nature was toxic either on animal or human and did not provide an advantage function biologically. Bacillus isolates S1 and SS19 known resistant to lead up to 50 mg / L PbCl2. In this research will be examined whether genera Bacillus isolates S1 and SS19 could accumulate metal lead (Pb), their capability in accumulating and profile protein differences when the bacteria genera Bacillus isolates S1 and SS19 get exposed metal lead (Pb). Inoculum at age ± 9 hours are used, with a Nutrient Broth (NB) containing 50, 75 and 100 mg / L PbCl2. Inductively Coupled Plasma Atomic Emission Spectrometry (ICP) used to assessed Pb2+ concentrations. Bioaccumulation levels of Pb2+ by Bacillus isolate S1 and SS19 related to the distinction of beginning concentration to the final concentration. Bacillus isolate S1 achieved 53% and 51% bioaccumulation efficiency rate in lead presence concentration (75 and 100 mg/L) and 51% (50 mg/L). Another way Bacillus isolate SS19 was able to accumulate 57% (50 mg/L PbCl2) and kept stable on 36% bioaccumulation efficiency rate (75 and 100 mg/L PbCl2). Regarding SDS-PAGE electrophoresis protein profile result, protein in ± 127 kDa, molecule mass detected in the presence of Lead for Bacillus isolate S1.

  8. Differential Role of the T6SS in Acinetobacter baumannii Virulence.

    Directory of Open Access Journals (Sweden)

    Guillermo D Repizo

    Full Text Available Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR strains. Herein, we compared a type strain (ATCC17978, a non-clinical isolate (DSM30011 and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825, revealing distinct patterns of type VI secretion system (T6SS functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner.

  9. GODDeSS: a Geant4 extension for easy modelling of optical detector components

    Science.gov (United States)

    Dietz-Laursonn, E.; Hebbeker, T.; Künsken, A.; Merschmeyer, M.; Nieswand, S.; Niggemann, T.

    2017-04-01

    Scintillator- and fibre-based particle detectors with SiPM readout are an indispensable tool in high-energy particle physics, medical physics and other fields of application. For designing and understanding these detectors, very detailed simulations are necessary, which require an accurate modelling of the optical physics (optics, scintillation, wavelength-shifting effects, ... ), of the optical material properties, and of the geometry. To allow for a reliable usage also by less experienced users, the necessary complexity and flexibility of a suitable simulation framework must not lead to an increasing danger of user mistakes. Additionally, the required effort for creating or modifying a detailed simulation has to be minimised in order to allow for the fast creation of flexible simulation setups. These challenges have been addressed by developing GODDeSS (Geant4 Objects for Detailed Detectors with Scintillators and SiPMs). It is an extension of the particle-physics simulation tool Geant4 and allows for the easy simulation of optical detector components, especially combinations of scintillators, optical fibres, and photodetectors. GODDeSS enables the user to create extensive setups for Geant4 simulations with a few lines of source code. At the same time, GODDeSS helps to avoid typical user mistakes. This paper introduces the basic concepts of the GODDeSS framework, its object classes, and its functionality. Furthermore, test measurements with prototype modules will be presented, which were performed to validate simulation results of the GODDeSS framework.

  10. Previously unknown and highly divergent ssDNA viruses populate the oceans.

    Science.gov (United States)

    Labonté, Jessica M; Suttle, Curtis A

    2013-11-01

    Single-stranded DNA (ssDNA) viruses are economically important pathogens of plants and animals, and are widespread in oceans; yet, the diversity and evolutionary relationships among marine ssDNA viruses remain largely unknown. Here we present the results from a metagenomic study of composite samples from temperate (Saanich Inlet, 11 samples; Strait of Georgia, 85 samples) and subtropical (46 samples, Gulf of Mexico) seawater. Most sequences (84%) had no evident similarity to sequenced viruses. In total, 608 putative complete genomes of ssDNA viruses were assembled, almost doubling the number of ssDNA viral genomes in databases. These comprised 129 genetically distinct groups, each represented by at least one complete genome that had no recognizable similarity to each other or to other virus sequences. Given that the seven recognized families of ssDNA viruses have considerable sequence homology within them, this suggests that many of these genetic groups may represent new viral families. Moreover, nearly 70% of the sequences were similar to one of these genomes, indicating that most of the sequences could be assigned to a genetically distinct group. Most sequences fell within 11 well-defined gene groups, each sharing a common gene. Some of these encoded putative replication and coat proteins that had similarity to sequences from viruses infecting eukaryotes, suggesting that these were likely from viruses infecting eukaryotic phytoplankton and zooplankton.

  11. Analysis of T4SS-induced signaling by H. pylori using quantitative phosphoproteomics

    Directory of Open Access Journals (Sweden)

    Frithjof eGlowinski

    2014-07-01

    Full Text Available Helicobacter pylori is a Gram-negative bacterial pathogen colonizing the human stomach. Infection with H. pylori causes chronic inflammation of the gastric mucosa and may lead to peptic ulceration and/or gastric cancer. A major virulence determinant of H. pylori is the type IV secretion system (T4SS, which is used to inject the virulence factor CagA into the host cell, triggering a wide range of cellular signaling events. Here, we used a phosphoproteomic approach to investigate tyrosine signaling in response to host-pathogen interaction, using stable isotope labeling in cell culture (SILAC of AGS cells to obtain a differential picture between multiple infection conditions. Cells were infected with wild type H. pylori P12, a P12ΔCagA deletion mutant, and a P12ΔT4SS deletion mutant to compare signaling changes over time and in the absence of CagA or the T4SS. Tryptic peptides were enriched for tyrosine (Tyr phosphopeptides and analysed by nano-LC-Orbitrap MS. In total, 58 different phosphosites were found to be regulated following infection. The majority of phosphosites identified were kinases of the MAPK familiy. CagA and the T4SS were found to be key regulators of Tyr phosphosites. Our findings indicate that CagA primarily induces activation of ERK1 and integrin linked factors, whereas the T4SS primarily modulates JNK and p38 activation.

  12. Potyvirus virion structure shows conserved protein fold and RNA binding site in ssRNA viruses.

    Science.gov (United States)

    Zamora, Miguel; Méndez-López, Eduardo; Agirrezabala, Xabier; Cuesta, Rebeca; Lavín, José L; Sánchez-Pina, M Amelia; Aranda, Miguel A; Valle, Mikel

    2017-09-01

    Potyviruses constitute the second largest genus of plant viruses and cause important economic losses in a large variety of crops; however, the atomic structure of their particles remains unknown. Infective potyvirus virions are long flexuous filaments where coat protein (CP) subunits assemble in helical mode bound to a monopartite positive-sense single-stranded RNA [(+)ssRNA] genome. We present the cryo-electron microscopy (cryoEM) structure of the potyvirus watermelon mosaic virus at a resolution of 4.0 Å. The atomic model shows a conserved fold for the CPs of flexible filamentous plant viruses, including a universally conserved RNA binding pocket, which is a potential target for antiviral compounds. This conserved fold of the CP is widely distributed in eukaryotic viruses and is also shared by nucleoproteins of enveloped viruses with segmented (-)ssRNA (negative-sense ssRNA) genomes, including influenza viruses.

  13. Sairaanhoitajan aseptinen työskentely heräämössä

    OpenAIRE

    Mäki, Sari; Lähteenmäki, Merja; Sinkko, Susanna

    2014-01-01

    Tämän opinnäytetyön aihe on sairaanhoitajan aseptinen työskentely heräämössä. Opinnäytetyö toteutettiin projektiyhteistyönä Laurea-ammattikorkeakoulun ja Naistenklinikan leikkaus- ja anestesiaosaston kanssa. Opinnäytetyön aihe työelämälähtöinen. Opinnäytetyön tarkoituksena on selvittää sairaanhoitajan aseptista työskentelyä heräämössä. Opinnäytetyön tutkimusmenetelmä on kvalitatiivinen. Tutkimusaineisto kerättiin avoimin kysymyksin lomakekyselynä kolmen eri sairaalan heräämössä työskentel...

  14. Combinatorial gene editing in mammalian cells using ssODNs and TALENs

    Science.gov (United States)

    Strouse, Bryan; Bialk, Pawel; Niamat, Rohina A.; Rivera-Torres, Natalia; Kmiec, Eric B.

    2014-01-01

    The regulation of gene editing is being elucidated in mammalian cells and its potential as well as its limitations are becoming evident. ssODNs carry out gene editing by annealing to their complimentary sequence at the target site and acting as primers for replication fork extension. To effect a genetic change, a large amount of ssODN molecules must be introduced into cells and as such induce a Reduced Proliferation Phenotype (RPP), a phenomenon in which corrected cells do not proliferate. To overcome this limitation, we have used TAL-Effector Nucleases (TALENs) to increase the frequency, while reducing the amount of ssODN required to direct gene correction. This strategy resolves the problem and averts the serious effects of RPP. The efficiency of gene editing can be increased significantly if cells are targeted while they progress through S phase. Our studies define new reaction parameters that will help guide experimental strategies of gene editing.

  15. FAST SS-ILM: A COMPUTATIONALLY EFFICIENT ALGORITHM TO DISCOVER SOCIALLY IMPORTANT LOCATIONS

    Directory of Open Access Journals (Sweden)

    A. S. Dokuz

    2017-11-01

    Full Text Available Socially important locations are places which are frequently visited by social media users in their social media lifetime. Discovering socially important locations provide several valuable information about user behaviours on social media networking sites. However, discovering socially important locations are challenging due to data volume and dimensions, spatial and temporal calculations, location sparseness in social media datasets, and inefficiency of current algorithms. In the literature, several studies are conducted to discover important locations, however, the proposed approaches do not work in computationally efficient manner. In this study, we propose Fast SS-ILM algorithm by modifying the algorithm of SS-ILM to mine socially important locations efficiently. Experimental results show that proposed Fast SS-ILM algorithm decreases execution time of socially important locations discovery process up to 20 %.

  16. Nucleation and growth microstructural study of ti films on 304 SS substrates

    Directory of Open Access Journals (Sweden)

    Rogério de Almeida Vieira

    2004-09-01

    Full Text Available Coating of steel surfaces with titanium films has been studied with the objective to protect them against corrosion, and to create an intermediate film for CVD diamond and TiN film deposition. In this work, the nucleation, growth mechanisms and microstructural formation of the titanium films deposited on 304 stainless steel (304 SS substrate are presented and discussed. The titanium films of variable thickness were obtained by vapour phase deposition produced by electron beam. The surfaces of these samples were observed by scanning electron microscopy. The cross sections of these samples were observed by using an atomic force microscope. The Ti film-304 SS interfaces were analyzed by X-ray diffraction. The results showed that titanium films have a columnar growth. The Ti film-304 SS interface had a residual compression stress at room temperature due to the inter-diffusion process.

  17. Ubuntu LTSP VirtualBox -ympäristössä

    OpenAIRE

    Jokinen, Mikko

    2016-01-01

    Työssä tutkitaan terminal server -järjestelmää virtuaaliympäristössä. Terminal server -järjestelmässä kaikki ohjelmat on asennettu yhdelle serverinä toimivalle tietokoneelle, jolloin käyttäjien tietokoneet ns. thin-clientit voivat olla suorituskyvyiltään vaatimattomia. Tällöin tehonkulutus on pienempi. Myös ylläpito on helpompaa, koska kaikki on vain yhdessä paikassa. Ubuntu LTSP -järjestelmä on Linux -pohjainen terminal server -järjestelmä. Tämä asenennetaan VirtualBox -ympäristöön. Vi...

  18. Anti-corrosive performance of electropolymerized phosphomolybdic acid doped PANI coating on 304SS

    Science.gov (United States)

    Gao, Yangzhi; Syed, Junaid Ali; Lu, Hongbin; Meng, Xiangkang

    2016-01-01

    A phosphomolybdic acid doped polyaniline (PANI) anti-corrosive coating was synthesized on stainless steel (304SS) by electropolymerization. The combination of phosphoric acid de-doping and phosphomolybdic acid re-doping processes leads to the direct and quick deposition of PANI-coating on 304SS without any residual oxidant impurities. The corrosion resistance of the coating was evaluated in 1 M H2SO4 solution by electrochemical techniques. The doping of phosphomolybdic acid enhances the corrosion resistance provided by the coating. The phosphomolybdic acid re-doped coating has a high Rt of 1999 Ω cm2 in comparison with the phosphoric acid doped and re-doped coatings. The increase in corrosion resistance is due to the presence of phosphomolybdic acid that promotes the formation of oxides which not only fill the pores in the PANI coating but also forms an interfacial oxide layer and hence block the corrosion of 304SS in acidic medium.

  19. Fast Ss-Ilm a Computationally Efficient Algorithm to Discover Socially Important Locations

    Science.gov (United States)

    Dokuz, A. S.; Celik, M.

    2017-11-01

    Socially important locations are places which are frequently visited by social media users in their social media lifetime. Discovering socially important locations provide several valuable information about user behaviours on social media networking sites. However, discovering socially important locations are challenging due to data volume and dimensions, spatial and temporal calculations, location sparseness in social media datasets, and inefficiency of current algorithms. In the literature, several studies are conducted to discover important locations, however, the proposed approaches do not work in computationally efficient manner. In this study, we propose Fast SS-ILM algorithm by modifying the algorithm of SS-ILM to mine socially important locations efficiently. Experimental results show that proposed Fast SS-ILM algorithm decreases execution time of socially important locations discovery process up to 20 %.

  20. Plasmon-driven dimerization via S-S chemical bond in an aqueous environment.

    Science.gov (United States)

    Cui, Lin; Wang, Peijie; Chen, Xiaowei; Fang, Yurui; Zhang, Zhenglong; Sun, Mengtao

    2014-11-27

    The surface-enhanced Raman scattering (SERS) spectra of thioanisole are experimentally investigated in an electrochemical environment in this study. Two Raman peaks, which depend strongly not only on electric potential but also on the local surface plasmon resonances (LSPR), have been observed. Theoretical calculations reveal that thioanisole is first dissociated from thiophenol via the S-CH3 bond; plasmons then drive the dimerisation of thiophenol via the S-S bond, which is strongly potential dependent. One Raman peak corresponds to the S-S vibrational mode of the thiophenol dimer, and the other corresponds to the asymmetric C-C stretching modes of the benzenyl of the thiophenol dimer. The potential-dependent two Raman modes is the potential-dependent dimerisation dynamics of thiophenol via the S-S bond. Our experimental findings provide insight into the structural elucidation of adsorbed molecules and molecular surface reaction dynamics.

  1. Automated SEM-EDS GSR Analysis for Turkish Ammunitions

    Science.gov (United States)

    Cakir, Ismail; Uner, H. Bulent

    2007-04-01

    In this work, Automated Scanning Electron Microscopy with Energy Dispersive X-ray Spectrometry (SEM-EDS) was used to characterize 7.65 and 9mm cartridges Turkish ammunition. All samples were analyzed in a SEM Jeol JSM-5600LV equipped BSE detector and a Link ISIS 300 (EDS). A working distance of 20mm, an accelerating voltage of 20 keV and gunshot residue software was used in all analysis. Automated search resulted in a high number of particles analyzed containing gunshot residues (GSR) unique elements (PbBaSb). The obtained data about the definition of characteristic GSR particles was concordant with other studies on this topic.

  2. New psych unit eases patient burden in ED.

    Science.gov (United States)

    2008-06-01

    Creating a special unit for psychiatric patients frees up beds in your ED, ensures better care for that patient population, and improves the atmosphere in the main department for the rest of your patients. Consider these suggestions: The creation of a psych unit can be extremely costly. Be prepared to demonstrate projected savings when pitching the idea to administration. Make sure an ED physician has overall responsibility for each patient at all times. Keep a regular group of nurses in rotation. This setup will help ensure smooth handoffs.

  3. Attenuation of the in vitro neurotoxicity of 316L SS by graphene oxide surface coating.

    Science.gov (United States)

    Tasnim, Nishat; Kumar, Alok; Joddar, Binata

    2017-04-01

    A persistent theme in biomaterials research comprises of surface engineering and modification of bare metallic substrates for improved cellular response and biocompatibility. Graphene Oxide (GO), a derivative of graphene, has outstanding chemical and mechanical properties; its large surface to volume ratio, ease of surface modification and processing make GO an attractive coating material. GO-coatings have been extensively studied as biosensors. Further owing to its surface nano-architecture, GO-coated surfaces promote cell adhesion and growth, making it suitable for tissue engineering applications. The need to improve the long-term durability and therapeutic effectiveness of commercially available bare 316L stainless steel (SS) surfaces led us to adopt a polymer-free approach which is cost-effective and scalable. GO was immobilized on to 316L SS utilizing amide linkage, to generate a strongly adherent uniform coating with surface roughness. GO-coated 316L SS surfaces showed increased hydrophilicity and biocompatibility with SHSY-5Y neuronal cells, which proliferated well and showed decreased reactive oxygen species (ROS) expression. In contrast, cells did not adhere to bare uncoated 316L SS meshes nor maintain viability when cultured in the vicinity of bare meshes. Therefore the combination of the improved surface properties and biocompatibility implies that GO-coating can be utilized to overcome pertinent limitations of bare metallic 316L SS implant surfaces, especially SS neural electrodes. Also, the procedure for making GO-based protective coatings can be applied to numerous other implants where the development of such protective films is necessary. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. Serum testosterone levels of HbSS (sickle cell disease male subjects in Lagos, Nigeria

    Directory of Open Access Journals (Sweden)

    Adediran Adewumi

    2011-08-01

    Full Text Available Abstract Background Infertility is a major problem in sickle cell disease patients, especially in males. In addition to low serum testosterone, other abnormalities involving the accessory sex organs, such as the seminal vesicles and the prostate gland, as well as marked decrease in ejaculate volume may be observed in male HbSS patients. Hence, the need to study the role of sex hormones as a cause of infertility in male HbSS patients. Methods An unmatched case-control study was performed using seventy-five consenting subjects from Lagos University Teaching Hospital. These included 47 patients with haemoglobin phenotype SS from the Sickle cell clinic and 28 volunteered medical students and members of staff with haemoglobin phenotype AA. Demographic data were obtained using a self-administered questionnaire. A total of 5 mls of blood was collected from each subject between 9.00 am & 11.am, and assayed for serum testosterone concentration. Results The concentrations of serum testosterone in HbSS patients ranged from 0.2 to 4.3 ng/ml with a mean of 1.28 ± 0.72 ng/ml whilst the values in HbAA controls ranged from 1.2 to 6.9 ng/ml with a mean of 2.63 ± 1.04 ng/ml. Seven (25.0% of the 28 controls had serum testosterone concentration lower than the quoted reference (normal range whereas 44 (93.6% of the 47 HbSS subjects had serum testosterone concentration lower than the reference range. Conclusion Overall, subjects with HbSS have significantly lower mean serum testosterone than HbAA controls.

  5. Phylogeny of Opuntia s.s. (Cactaceae): clade delineation, geographic origins, and reticulate evolution.

    Science.gov (United States)

    Majure, Lucas C; Puente, Raul; Griffith, M Patrick; Judd, Walter S; Soltis, Pamela S; Soltis, Douglas E

    2012-05-01

    The opuntias (nopales, prickly pears) are not only culturally, ecologically, economically, and medicinally important, but are renowned for their taxonomic difficulty due to interspecific hybridization, polyploidy, and morphological variability. Evolutionary relationships in these stem succulents have been insufficiently studied; thus, delimitation of Opuntia s.s. and major subclades, as well as the biogeographic history of this enigmatic group, remain unresolved. We sequenced the plastid intergenic spacers atpB-rbcL, ndhF-rpl32, psbJ-petA, and trnL-trnF, the plastid genes matK and ycf1, the nuclear gene ppc, and ITS to reconstruct the phylogeny of tribe Opuntieae, including Opuntia s.s. We used phylogenetic hypotheses to infer the biogeographic history, divergence times, and potential reticulate evolution of Opuntieae. Within Opuntieae, a clade of Tacinga, Opuntia lilae, Brasiliopuntia, and O. schickendantzii is sister to a well-supported Opuntia s.s., which includes Nopalea. Opuntia s.s. originated in southwestern South America (SA) and then expanded to the Central Andean Valleys and the desert region of western North America (NA). Two major clades evolved in NA, which subsequently diversified into eight subclades. These expanded north to Canada and south to Central America and the Caribbean, eventually returning back to SA primarily via allopolyploid taxa. Dating approaches suggest that most of the major subclades in Opuntia s.s. originated during the Pliocene. Opuntia s.s. is a well-supported clade that includes Nopalea. The clade originated in southwestern SA, but the NA radiation was the most extensive, resulting in broad morphological diversity and frequent species formation through reticulate evolution and polyploidy.

  6. Protective effects of SS31 on t-BHP induced oxidative damage in 661W cells

    Science.gov (United States)

    MA, WEI; ZHU, XIAOBO; DING, XIAOYAN; LI, TAO; HU, YIJUN; HU, XUTING; YUAN, LIN; LEI, LEI; HU, ANDINA; LUO, YAN; TANG, SHIBO

    2015-01-01

    The present study aimed to investigate the ability of SS31, a novel mitochondria-targeted peptide to protect against t-BHP-induced mitochondrial dysfunction and apoptosis in 661W cell lines. The 661W cells were treated with various concentrations of SS-31 and an MTT assay was used to determine cell viability. The expression of nitrotyrosine and 8-hydroxydeoxyguanosine (8-OHdG) was detected using immunofluorescent staining. Apoptosis were assessed using Hoechst staining and an annexin V/propidium iodide flow cytometer. Reactive oxygen species (ROS) were detected using MitoSOX™ with confocal microscopy. Changes in mitochondrial membrane potential were analyzed using flow cytometry. In addition, the release of cytochrome c was analyzed using confocal microscopy. The viability of the cells improved following treatment with SS31 between 100 nM and 1 µM, compared with untreated control group. Compared with the t-BHP treatment group (20.0±3.8%), the number of annexin V-positive cells decreased dose-dependently to 13.6±2.6, 9.8±0.5 and 7.4±2.0% in the SS-31 treated group at concentrations of 10 nM, 100 nM and 1 µM, respectively. Treatment with SS-31 significantly prevented the t-BHP-induced expression of nitrotyrosine and 8-OHdG, decreased the quantity of mitochondrial ROS, increased mitochondrial potential, and prevented the release of cytochrome c from mitochondria into the cytoplasm. Therefore, the SS31 mitochondria-targeted peptide protected the 661W cells from the sustained oxidative stress induced by t-BHP. PMID:26165373

  7. Sukupolvenvaihdoksen verosuunnittelu Autoväri Keijo Hirvonen Oy:ssä

    OpenAIRE

    Vauhkonen, Sanna

    2013-01-01

    Pretax Pohjois-Karjala Oy oli opinnäytetyön toimeksiantaja, jonka asiakasyritykselle Autoväri Keijo Hirvonen Oy:lle tehtiin opinnäytetyö. Sukupolvenvaihdos Autoväri Keijo Hirvonen Oy:ssä on tarkoitus toteuttaa kolmessa eri vaiheessa. Kaksi vaiheista on jo toteutettu ja viimeinen sukupolvenvaihdoksen vaihde ollaan toteuttamassa viiden vuoden sisällä. Opinnäytetyön tarkoituksena oli selvittää verotuksellisesti edullisin vastikkeeton suku-polvenvaihdoksen muoto. Opinnäytetyössä tutkittavia l...

  8. Truncated SSX protein suppresses synovial sarcoma cell proliferation by inhibiting the localization of SS18-SSX fusion protein.

    Directory of Open Access Journals (Sweden)

    Yasushi Yoneda

    Full Text Available Synovial sarcoma is a relatively rare high-grade soft tissue sarcoma that often develops in the limbs of young people and induces the lung and the lymph node metastasis resulting in poor prognosis. In patients with synovial sarcoma, specific chromosomal translocation of t(X; 18 (p11.2;q11.2 is observed, and SS18-SSX fusion protein expressed by this translocation is reported to be associated with pathogenesis. However, role of the fusion protein in the pathogenesis of synovial sarcoma has not yet been completely clarified. In this study, we focused on the localization patterns of SS18-SSX fusion protein. We constructed expression plasmids coding for the full length SS18-SSX, the truncated SS18 moiety (tSS18 and the truncated SSX moiety (tSSX of SS18-SSX, tagged with fluorescent proteins. These plasmids were transfected in synovial sarcoma SYO-1 cells and we observed the expression of these proteins using a fluorescence microscope. The SS18-SSX fusion protein showed a characteristic speckle pattern in the nucleus. However, when SS18-SSX was co-expressed with tSSX, localization of SS18-SSX changed from speckle patterns to the diffused pattern similar to the localization pattern of tSSX and SSX. Furthermore, cell proliferation and colony formation of synovial sarcoma SYO-1 and YaFuSS cells were suppressed by exogenous tSSX expression. Our results suggest that the characteristic speckle localization pattern of SS18-SSX is strongly involved in the tumorigenesis through the SSX moiety of the SS18-SSX fusion protein. These findings could be applied to further understand the pathogenic mechanisms, and towards the development of molecular targeting approach for synovial sarcoma.

  9. Auto-imagen y estabilidad emocional de homosexuales "edípicos" y "no edípicos"

    Directory of Open Access Journals (Sweden)

    Peter O. Peretti

    1976-01-01

    Full Text Available The past several years have brought about some changes in the legal status of the homosexual. In some states of the USA, the homosexual ís labelled as a criminal, in others homosexualíty is not a legal affair. Psychological theories tend to consider the homosexual from two general positions the "Oedipal" and "non-Oedípal", The present study was conducted to try to determine how "Oedipal" and "non-Oedipal" male hornosexuals perceíved their self-imageand emotional stabilíty. Significant differences were found between the twogroups. Considering self-image, "Oedipal" Ss were found tI:! be more negative. They had less self-worth, self confidence and self acceptance than "non-Oedipal". They had more negative self-concepts, self-atritudes, and self-motivation. "Oedipal" Ss had lesa emotional stability, They had more emotional problema, shame, guilt, withdrawal. and depression than "non-Oedípal" Ss; as well as less success in sexual activity and commitment to the homosexual partner.

  10. Structural requirements for the interaction between peptide antigens and I-Ed molecules

    DEFF Research Database (Denmark)

    Sette, A; Adorini, L; Appella, E

    1989-01-01

    amino acid substitution analogues of one good I-Ed binder, hen egg lysozyme 107-116, were analyzed for their capacity to bind to I-Ed molecules and to activate two different I-Ed-restricted T cell hybridomas. The results illustrate the great permissiveness of I-Ed-peptide interaction and the great......We have analyzed the structural characteristics of the interaction between I-Ed molecules and their peptide ligands. It was found that unrelated good I-Ed binders share structurally similar "core" regions that were experimentally demonstrated to be crucial for binding to I-Ed molecules. Single...

  11. Effect of Laser Surface Melting on the Microstructure and Pitting Corrosion Resistance of 304L SS Weldment

    Science.gov (United States)

    Suresh, Girija; Dasgupta, Arup; Kishor, P. S. V. R. A.; Upadhyay, B. N.; Saravanan, T.; Mallika, C.; Mudali, U. Kamachi

    2017-10-01

    The manuscript presents the effect of laser surface melting (LSM) on the microstructural variations and pitting corrosion resistance of 304L SS weldment fabricated by gas tungsten arc welding of 304L SS plates using 308L SS filler wire. The weld region was examined by X-ray radiography for defect detection. LSM of 304L SS weldment was performed using Nd:YAG pulsed laser. Microstructural evaluation was carried out using optical and electron back scatter diffraction techniques. The microstructure of 304L SS base was found to be austenitic, while the weld region of 304L SS weldment contained delta ferrite distributed in austenite matrix. The microstructure of LSM 304L SS weldment was found to be homogeneous austenite matrix with sparsely distributed ferrite. Ferrite measurements showed a decrease in the percentage ferrite in the fusion zone of 304L SS weldment after LSM. A profound enhancement in the pitting corrosion resistance was observed after LSM, which could be attributed to the homogeneous microstructure and decrease in the ferrite content. Pit density was found to be higher in the heat-affected zone of the weldment. Very few pits were observed in the LSM 304L SS weldment compared to the as-weldment.

  12. Inside the Sex Ed Studio: An Interview with Peggy Brick

    Science.gov (United States)

    Taverner, William J.

    2008-01-01

    "Inside the Sex Ed Studio" profiles leaders in the field of sexuality education. Peggy Brick, former director of Planned Parenthood of Greater Northern New Jersey's Center for Family Life Education (CFLE) and author of numerous sexuality education resources used worldwide, is the subject of this interview. Ms. Brick was interviewed by…

  13. Psychological and psychopathological variables associated with eating disorders (ED

    Directory of Open Access Journals (Sweden)

    Antonio Fernández-Delgado

    2016-07-01

    Full Text Available Objective: The aim of this study was to compare some psychological and psychopathological variables usually associated with different types of patients with eating disorders (ED. A total of 22 variables (psychological, psychopathological and specifically related to TCA were analyzed in three groups of patients with anorexia nervosa (AN, bulimia nervosa (BN and eating disorders not otherwise specified (EDNOS. Method: The sample consisted of 76 patients diagnosed with ED (mean age 20.13 ± 6.28 years; 69 women and 7 men. The following questionnaires were administered: Rosenberg's Self-Esteem Scale (SES, Symptom Checklist-90-Revised (SCL-90-R, Beck's Depression Inventory (BDI, State-Trait Anxiety Inventory (STAI, Body Shape Questionnaire (BSQ, Body Appreciation Scale (BAS, Eating Disorder Inventory-2 (EDI-2 and Body Image Quality of Life Inventory (BIQLI-SP. Results: Generally no significant differences between groups, except for the variables related to the BSQ and EDI-2 questionnaires, were found. The study of the correlations among the different variables specifically related to eating disorders and others, showed differences between groups. Conclusions: The present study shows few differences with respect to psychopathological symptoms among the different types of ED. Bearing in mind future studies, it would be interesting to use a bigger sample size, to include more men, and to distinguish between restricted/purging types of ED.

  14. Diagnostic utility of the genital Gram stain in ED patients.

    Science.gov (United States)

    Stefanski, Peter; Hafner, John W; Riley, Shanda L; Sunga, Kharmene L Y; Schaefer, Timothy J

    2010-01-01

    The study aimed to determine the diagnostic usefulness of the genital Gram stain in an emergency department (ED) population. A linked-query of an urban, tertiary-care, university- affiliated hospital laboratory database was conducted for all completed Chlamydia trachomatis and Neisseria gonorrhoeae DNA probes, Trichomonas vaginalis wet preps, and genital Gram stains performed on ED patient visits between January and December 2004. Positive criteria for a Gram stain included greater than 10 white blood cells per high-power field, gram-negative intracellular/extracellular diplococci (suggesting N gonorrhoeae), clue cells (suggesting T vaginalis), or direct visualization of T vaginalis organisms. DNA probes were used as the gold standard definition for N gonorrhoeae and C trachomatis infection. Of 1511 initially eligible ED visits, 941 were analyzed (genital Gram stain and DNA probe results both present), with a prevalence of either C trachomatis or N gonorrhoeae of 11.4%. A positive genital Gram stain was 75.7% sensitive and 43.3% specific in diagnosing either C trachomatis and/or N gonorrhoeae infection, and 80.4% sensitive and 32.2% specific when the positive cutoff was lowered to more than 5 white blood cells/high-power field. No Gram stains were positive for T vaginalis (with 47 positive wet mounts), and clue cells were noted on 117 Gram stains (11.6%). Gram stains in isolation lack sufficient diagnostic ability to detect either C trachomatis or N gonorrhoeae infection in the ED.

  15. I>Lo specchio frammentato ed il pagliaccio piangente : alienation ...

    African Journals Online (AJOL)

    Quest'articolo esamina il modo in cui il cinema di Nanni Moretti usa uno sguardo autoriflessivamente critico per parlare della condizione frammentata ed alienata di un soggetto maschile, italiano e di sinistra, nella tarda età capitalista. L'articolo mette a fuoco come l'uso morettiano dell' umorismo, con palesi tratti ...

  16. Mai alguses linastub dokfilm "Sinimäed" / Andris Feldmanis

    Index Scriptorium Estoniae

    Feldmanis, Andris, 1982-

    2006-01-01

    Valminud on 6-osalise ajaloodokumentaalide sarja "Kuum külm sõda" teine film "Sinimäed" : stsenaristid Kiur Aarma, Mart Laar, Eerik-Niiles Kross, Raimo Jõerand : režissöör Raimo Jõerand : Ruut Pictures

  17. Creating a Minnesota Statewide SNAP-Ed Program Evaluation

    Science.gov (United States)

    Gold, Abby; Barno, Trina Adler; Sherman, Shelley; Lovett, Kathleen; Hurtado, G. Ali

    2013-01-01

    Systematic evaluation is an essential tool for understanding program effectiveness. This article describes the pilot test of a statewide evaluation tool for the Supplemental Nutrition Assistance Program-Education (SNAP-Ed). A computer algorithm helped Community Nutrition Educators (CNEs) build surveys specific to their varied educational settings…

  18. Murray Pittock, ed., The Edinburgh Companion to Scottish Romanticism.

    Directory of Open Access Journals (Sweden)

    Manfred Malzahn

    2013-10-01

    Full Text Available Murray Pittock, ed., The Edinburgh Companion to Scottish Romanticism. Edinburgh: Edinburgh University Press, 2011. Pp. 251. ISBN 978-0-7486-3845-1 (hardback. £ 65.00. ISBN 978-0-7486-3846-8 (paperback. £ 21.99.

  19. Impact of Physician Navigators on productivity indicators in the ED.

    Science.gov (United States)

    Leung, Alexander K; Puri, Gaurav; Chen, Bingshu E; Gong, Zhenxian; Chan, Eddie; Feng, Edward; Duic, Marko

    2018-01-01

    We created Physician Navigators in our ED to help improve emergency physician (EP) productivity. We aimed to quantify the effect of Physician Navigators on measures of EP productivity: patient seen per hour (Pt/hr), and turn-around time (TAT) to discharge. Secondary objectives included examining their impact on measures of ED throughput for non-resuscitative patients: ED length of stay (LOS), door-to-physician time and left-without-being-seen rates (LWBS). In this retrospective study, 6845 clinical shifts worked by 20 EPs at a community ED in Newmarket, Canada from 1 January 2012 to 31 March 2015 were evaluated. Using a clustered design, we compared productivity measures between shifts with and without Physician Navigators, by physician. We used a linear mixed model to examine mean changes in Pt/hr and TAT to discharge for EPs who employed Physician Navigators. For secondary objectives, autoregressive modelling was performed to compare ED throughput metrics before and after the implementation of Physician Navigators for non-resuscitative patients. Patient volumes increased by 20 patients per day (p<0.001). Mean Pt/hr increased by 1.07 patients per hour (0.98 to 1.16, p<0.001). The mean TAT to discharge decreased by 10.6 min (-13.2 to -8.0, p<0.001). After implementation of the Physician Navigator programme, overall mean LOS for non-resuscitative patients decreased by 2.6 min (p=0.007), and mean door-to-physician time decreased by 7.4 min (p<0.001). LBWS rates decreased from 1.13% to 0.63% of daily patient volume (p<0.001). Despite an ED volume increase, the use of a Physician Navigator was associated with significant improvements in EP productivity, and significant reductions in ED throughput times. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

  20. Developmental and hormonally regulated messenger ribonucleic acid expression of KiSS-1 and its putative receptor, GPR54, in rat hypothalamus and potent luteinizing hormone-releasing activity of KiSS-1 peptide.

    Science.gov (United States)

    Navarro, V M; Castellano, J M; Fernández-Fernández, R; Barreiro, M L; Roa, J; Sanchez-Criado, J E; Aguilar, E; Dieguez, C; Pinilla, L; Tena-Sempere, M

    2004-10-01

    The gonadotropic axis is centrally controlled by a complex regulatory network of excitatory and inhibitory signals that is activated at puberty. Recently, loss of function mutations of the gene encoding G protein-coupled receptor 54 (GPR54), the putative receptor for the KiSS-1-derived peptide metastin, have been associated with lack of puberty onset and hypogonadotropic hypogonadism. Yet the pattern of expression and functional role of the KiSS-1/GPR54 system in the rat hypothalamus remain unexplored to date. In the present work, expression analyses of KiSS-1 and GPR54 genes were conducted in different physiological and experimental settings, and the effects of central administration of KiSS-1 peptide on LH release were assessed in vivo. Persistent expression of KiSS-1 and GPR54 mRNAs was detected in rat hypothalamus throughout postnatal development, with maximum expression levels at puberty in both male and female rats. Hypothalamic expression of KiSS-1 and GPR54 genes changed throughout the estrous cycle and was significantly increased after gonadectomy, a rise that was prevented by sex steroid replacement both in males and females. Moreover, hypothalamic expression of the KiSS-1 gene was sensitive to neonatal imprinting by estrogen. From a functional standpoint, intracerebroventricular administration of KiSS-1 peptide induced a dramatic increase in serum LH levels in prepubertal male and female rats as well as in adult animals. In conclusion, we provide novel evidence of the developmental and hormonally regulated expression of KiSS-1 and GPR54 mRNAs in rat hypothalamus and the ability of KiSS-1 peptide to potently stimulate LH secretion in vivo. Our current data support the contention that the hypothalamic KiSS-1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.

  1. TrED: the Trichophyton rubrum Expression Database

    Directory of Open Access Journals (Sweden)

    Liu Tao

    2007-07-01

    Full Text Available Abstract Background Trichophyton rubrum is the most common dermatophyte species and the most frequent cause of fungal skin infections in humans worldwide. It's a major concern because feet and nail infections caused by this organism is extremely difficult to cure. A large set of expression data including expressed sequence tags (ESTs and transcriptional profiles of this important fungal pathogen are now available. Careful analysis of these data can give valuable information about potential virulence factors, antigens and novel metabolic pathways. We intend to create an integrated database TrED to facilitate the study of dermatophytes, and enhance the development of effective diagnostic and treatment strategies. Description All publicly available ESTs and expression profiles of T. rubrum during conidial germination in time-course experiments and challenged with antifungal agents are deposited in the database. In addition, comparative genomics hybridization results of 22 dermatophytic fungi strains from three genera, Trichophyton, Microsporum and Epidermophyton, are also included. ESTs are clustered and assembled to elongate the sequence length and abate redundancy. TrED provides functional analysis based on GenBank, Pfam, and KOG databases, along with KEGG pathway and GO vocabulary. It is integrated with a suite of custom web-based tools that facilitate querying and retrieving various EST properties, visualization and comparison of transcriptional profiles, and sequence-similarity searching by BLAST. Conclusion TrED is built upon a relational database, with a web interface offering analytic functions, to provide integrated access to various expression data of T. rubrum and comparative results of dermatophytes. It is devoted to be a comprehensive resource and platform to assist functional genomic studies in dermatophytes. TrED is available from URL: http://www.mgc.ac.cn/TrED/.

  2. Brasiliocroton : a new crotonoid genus of Euphorbiaceae s.s. from eastern Brazil

    Science.gov (United States)

    Paul E. Berry; Ines Cordeiro; Alex C. Wiedenhoeft; Maria Amelia Vitorino-Cruz; Leticia Ribes de Lima

    2005-01-01

    A new genus and species of Euphorbiaceae s.s., Brasiliocroton mamoninha, is described from two disjunct areas of lowland forest remnants in eastern and northeastern Brazil. It is a member of tribe Crotoneae and was previously confused with Croton and Micrandra. The resemblance to Micrandra is based on the branched inflorescences and terminal position of the pistillate...

  3. Is PiSS Alpha-1 Antitrypsin Deficiency Associated with Disease?

    Directory of Open Access Journals (Sweden)

    Dawn McGee

    2010-01-01

    Full Text Available Background. Alpha-1 antitrypsin deficiency (AAT is an inherited condition that predisposes to lung and/or liver disease. Objective. The current study examined the clinical features of the PiSS genotype. Methods. Nineteen study participants (PiSS and 29 matched control participants (PiMM were telephone interviewed using a standardized questionnaire. Demographic features, cigarette smoking, vocation, medication history, and clinical diagnoses were compared. Statistical analysis was performed. Finally, a comprehensive literature review was performed by two investigators. Results. 12/19 (63.2% study participants reported the presence of lung and/or liver disease compared to 12/29 (41.4% control participants. There trended toward having a higher frequency of medication allergies in the study population (42.11% versus 20.69%. Conclusions. The PiSS genotype was associated with a similar incidence of obstructive lung disease to controls. Selective bias intrinsic in testing for AAT deficiency and the rarity of the PiSS genotype will make future study of this association dependent on population-based tests.

  4. Is PiSS Alpha-1 Antitrypsin Deficiency Associated with Disease?

    Science.gov (United States)

    McGee, Dawn; Schwarz, Laura; McClure, Rebecca; Peterka, Lauren; Rouhani, Farshid; Brantly, Mark; Strange, Charlie

    2010-01-01

    Background. Alpha-1 antitrypsin deficiency (AAT) is an inherited condition that predisposes to lung and/or liver disease. Objective. The current study examined the clinical features of the PiSS genotype. Methods. Nineteen study participants (PiSS) and 29 matched control participants (PiMM) were telephone interviewed using a standardized questionnaire. Demographic features, cigarette smoking, vocation, medication history, and clinical diagnoses were compared. Statistical analysis was performed. Finally, a comprehensive literature review was performed by two investigators. Results. 12/19 (63.2%) study participants reported the presence of lung and/or liver disease compared to 12/29 (41.4%) control participants. There trended toward having a higher frequency of medication allergies in the study population (42.11% versus 20.69%). Conclusions. The PiSS genotype was associated with a similar incidence of obstructive lung disease to controls. Selective bias intrinsic in testing for AAT deficiency and the rarity of the PiSS genotype will make future study of this association dependent on population-based tests. PMID:21687342

  5. A Bio-Electro-Fenton System Employing the Composite FePc/CNT/SS316 Cathode

    Directory of Open Access Journals (Sweden)

    Yi-Ta Wang

    2017-02-01

    Full Text Available Bio-electro-Fenton microbial fuel cells generate energy through the decomposition of organic matter by microorganisms. The generated electricity drives a Fenton reaction in a cathode chamber, which can be used for the decolorization of dye wastewater. Most of the previous works added expensive platinum catalyst to improve the electrical property of the system. In this research, aligned carbon nanotubes (CNTs were generated on the surface of SS316 stainless steel by chemical vapor deposition, and an iron phthalocyanine (FePc catalyst was added to fabricate a compound (FePc/CNT/SS316 that was applied to the cathode electrode of the fuel cell system. This was expected to improve the overall electricity generation efficiency and extent of decolorization of the system. The results showed that the maximum current density of the system with the modified electrode was 3206.30 mA/m2, and the maximum power was 726.55 mW/m2, which were increased by 937 and 2594 times, respectively, compared to the current and power densities of a system where only the SS316 stainless steel electrode was used. In addition, the decolorization of RB5 dye reached 84.6% within 12 h. Measurements of the electrical properties of bio-electro-Fenton microbial fuel cells and dye decolorization experiments with the FePc/CNT/SS316 electrode showed good results.

  6. Statement of Retraction: Kumar Ajay, Singh Neetu, Gaurav S.S. ...

    African Journals Online (AJOL)

    McRoy

    The Editors and Publisher of the International Journal of Medicine and Biomedical Research wish to state that the following article has been retracted from the journal: Kumar Ajay, Singh. Neetu, Gaurav S.S. Forensic Bioinformatics: An innovative technological advancement in the field of Forensic Medicine and Diagnosis.

  7. High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

    DEFF Research Database (Denmark)

    Chen, Fuqiang; Pruett-Miller, Shondra M; Huang, Yuping

    2011-01-01

    Zinc-finger nucleases (ZFNs) have enabled highly efficient gene targeting in multiple cell types and organisms. Here we describe methods for using simple ssDNA oligonucleotides in tandem with ZFNs to efficiently produce human cell lines with three distinct genetic outcomes: (i) targeted point...

  8. 20 CFR 225.24 - SS Earnings PIA used in survivor annuities.

    Science.gov (United States)

    2010-04-01

    ... the Social Security Act as in effect on December 31, 1974. It is computed using the deceased employee... RETIREMENT ACT PRIMARY INSURANCE AMOUNT DETERMINATIONS PIA's Used in Computing Survivor Annuities and the Amount of the Residual Lump-Sum Payable § 225.24 SS Earnings PIA used in survivor annuities. The Social...

  9. Validity of automated choroidal segmentation in SS-OCT and SD-OCT

    NARCIS (Netherlands)

    L. Zhang (Li); G.H.S. Buitendijk (Gabrielle); K. Lee (Kyungmoo); M. Sonka (Milan); H. Springelkamp (Henriët); A. Hofman (Albert); J.R. Vingerling (Hans); R.F. Mullins (Robert F.); C.C.W. Klaver (Caroline); M.D. Abràmoff (Michael)

    2015-01-01

    textabstractPURPOSE. To evaluate the validity of a novel fully automated three-dimensional (3D) method capable of segmenting the choroid from two different optical coherence tomography scanners: swept-source OCT (SS-OCT) and spectral-domain OCT (SD-OCT). METHODS. One hundred eight subjects were

  10. BER analysis of SS-WDM based FSO system for Vellore weather conditions

    Science.gov (United States)

    Prabu, K.; Charanya, S.; Jain, Mehul; Guha, Debapriya

    2017-11-01

    Spectrum Slicing Wavelength Division Multiplexing (SS-WDM) has been advanced to boost the long distance communication in FSO channel which assists immense bit rate due to its high capacity and efficiency. It is a substitute to WDM systems having multiple coherent lasers with different wavelengths which is expensive. This paper is subjected to investigate the characteristics and quality of communication links of data rate 1.56 GB/s and wavelength of 1550 nm. The SS-WDM technique has been inspected for 4, 8 and 16 channels to increase the performance of communication under various weather conditions. The proposed model is susceptible to system degradation due to turbulences where wind velocity, refractive index and height of buildings have been majorly focused. A case study has been experimented on how the height of buildings around VIT, Vellore campus interfere the transmission of light in free space. Based on the above study, the results shows that the benefits of spectrum slicing wavelength division multiplexing (SS-WDM) have been worked up on for the climatic conditions which enhance performance of system. Moreover, the graphs plotted against bit error rate (BER), attenuation and distance makes the analysis better which highlights the characteristic features of SS-WDM.

  11. ss Judutarra filma. III. Reich-eko zine propagandako trilogia antisemita

    OpenAIRE

    Díaz Ugarte, Eneko

    2016-01-01

    [EUS] Alemaniar III. Reich-eko garaian ikusentzuneko porpagandaren arloan hanibat film estreinatu ziren. Horietako bat Adibide modura hartuz, Süss Judutarra, lanean Goebbelsek Der Angriff egunkarian argitaratutako judutarren dekalogoa eta NSDAPko 25 puntuetan agertzen den antisemitismoa filman nola islatzen den aztertuko da.

  12. Characterization of Circular ssDNA Viruses within the Echinoderm Nanobiome

    Science.gov (United States)

    Jackson, E.; Bistolas, K. S.; Hewson, I.

    2016-02-01

    Viral metagenomics has revealed a great diversity and presence of circular single-stranded(ss) DNA viruses most similar to the viral family Circoviridae in various environments both ambient and host. These viruses are an emerging paradigm in viral discovery amongst aquatic invertebrates mainly from the sub-phlya Crustacea and to a lesser extent the phylum Echinodermata. This parasite-host relationship is furthered here with the discovery of circo-like viruses extracted from the tissue of members from the family Holothuroidea (sea cucumbers). Verification and presence of these viruses within the tissue of the host was confirmed through rigorous genome architecture screening and PCR amplification of the rep gene from unamplified viral DNA extracts. Phylogenetic analysis of the rep gene reveals high similarity to circular ssDNA viruses from environmental metagenomic surveys of marine habitats. The significance of these findings is changing the perception and understanding of circular ssDNA viruses by broadening the known host range and blurring certain defining characteristics established by their pathogenic counterparts. Aside from discover and characterization, the potential ecological impacts of ssDNA viruses upon their host remains relatively unknown and further investigations should aim to determine the pathology, route of infection, and ecological implications of viral infection.

  13. Pengaruh penggunaan kuning telur dan polarasol SS terhadap kemuluran kulit kras kambing

    Directory of Open Access Journals (Sweden)

    Bambang Oetojo

    1998-06-01

    Full Text Available The objective of this research is to know the influence of using egg yolk and Polarasol SS in fatliquoring process to the stretch of the goat crust leather. It was used 18 pieces of goat wet blue skin in this research. They were neutralized so as to the pH 6.0 – 6.5. A part of goat wet blue skins were fatliquored with egg yolk 5.0%, 7.5% and 10% successively. The other part was fatliquored using Polarasol SS by the same manner to that one of using egg yolk. The subsequence process were fixation, aging, sammying, drying, conditioning, sticking and taking. The goat crust leathers output were visual investigated for the appearance, softness and smoothness. Whereas physical testing for the stretch of those ones. Practical meaning of this research is, fatliquoring process using egg yolk gives a cleaner grain surface and smoother goat crust leather than using Polarasol SS. Whereas Polarasol SS gives softer and higher stretch of goat crust leather than using egg yolk.

  14. Screening and Identification of ssDNA Aptamer for Human GP73

    Directory of Open Access Journals (Sweden)

    Jingchun Du

    2015-01-01

    Full Text Available As one tumor marker of HCC, Golgi Protein 73 (GP73 is given more promise in the early diagnosis of HCC, and aptamers have been developed to compete with antibodies as biorecognition probes in different detection system. In this study, we utilized GP73 to screen specific ssDNA aptamers by SELEX technique. First, GP73 proteins were expressed and purified by prokaryotic expression system and Nickle ion affinity chromatography, respectively. At the same time, the immunogenicity of purified GP73 was confirmed by Western blotting. The enriched ssDNA library with high binding capacity for GP73 was obtained after ten rounds of SELEX. Then, thirty ssDNA aptamers were sequenced, in which two ssDNA aptamers with identical DNA sequence were confirmed, based on the alignment results, and designated as A10-2. Furthermore, the specific antibody could block the binding of A10-2 to GP73, and the specific binding of A10-2 to GP73 was also supported by the observation that several tumor cell lines exhibited variable expression level of GP73. Significantly, the identified aptamer A10-2 could distinguish normal and cancerous liver tissues. So, our results indicate that the aptamer A10-2 might be developed into one molecular probe to detect HCC from normal liver specimens.

  15. Metric-enriched categories and approximate Fra\\"{i}ss\\'{e} limits

    OpenAIRE

    Kubiś, Wiesław

    2012-01-01

    We develop the theory of approximate Fra\\"{i}ss\\'{e} limits in the context of categories enriched over metric spaces. Among applications, we construct a generic projection on the Gurarii space and we present a simpler proof of a characterization of the pseudo-arc, due to Irwin and Solecki.

  16. PU/SS EUTECTIC ASSESSMENT IN 9975 PACKAGINGS IN A STORAGE FACILITY DURING EXTENDED FIRE

    Energy Technology Data Exchange (ETDEWEB)

    Gupta, N.

    2012-03-26

    In a radioactive material (RAM) packaging, the formation of eutectic at the Pu/SS (plutonium/stainless steel) interface is a serious concern and must be avoided to prevent of leakage of fissile material to the environment. The eutectic temperature for the Pu/SS is rather low (410 C) and could seriously impact the structural integrity of the containment vessel under accident conditions involving fire. The 9975 packaging is used for long term storage of Pu bearing materials in the DOE complex where the Pu comes in contact with the stainless steel containment vessel. Due to the serious consequences of the containment breach at the eutectic site, the Pu/SS interface temperature is kept well below the eutectic formation temperature of 410 C. This paper discusses the thermal models and the results for the extended fire conditions (1500 F for 86 minutes) that exist in a long term storage facility and concludes that the 9975 packaging Pu/SS interface temperature is well below the eutectic temperature.

  17. Optimal continuous order quantity (s,S) policies; the 45-degrees algorithm

    NARCIS (Netherlands)

    E.M. Bazsa-Oldenkamp; P. den Iseger

    2002-01-01

    textabstractThe most recent optimization algorithm for (s,S) order policies with continuous demand was developed by Federgruen and Zipkin (1985). This was also the first efficient algorithm, which uses policy iteration instead of discretization. Zheng and Federgruen (1991) developed an even more

  18. Evading plant immunity: feedback control of the T3SS in Pseudomonas syringae

    Science.gov (United States)

    Waite, Christopher; Schumacher, Jörg; Jovanovic, Milija; Bennett, Mark; Buck, Martin

    2017-01-01

    Microbes are responsible for over 10% of the global yield losses in staple crops such as wheat, rice, and maize. Understanding the decision-making strategies that enable bacterial plant pathogens to evade the host immune system and cause disease is essential for managing their ever growing threat to food security. Many utilise the needle-like type III secretion system (T3SS) to suppress plant immunity, by injecting effector proteins that inhibit eukaryotic signalling pathways into the host cell cytoplasm. Plants can in turn evolve resistance to specific pathogens via recognition and blocking of the T3SS effectors, so leading to an ongoing co-evolutionary ‘arms race’ between pathogen and host pairs. The extracytoplasmic function sigma factor HrpL co-ordinates the expression of the T3SS regulon in the leaf-dwelling Pseudomonas syringae and similar pathogens. Recently, we showed that association of HrpL with a target promoter directly adjacent to the hrpL gene imposes negative autogenous control on its own expression level due to overlapping regulatory elements. Our results suggest that by down-regulating T3SS function, this fine-tuning mechanism enables P. syringae to minimise effector-mediated elicitation of plant immunity. PMID:28435841

  19. Fatigue Crack Propagation from Notched Specimens of 304 SS in elevated Temperature Aqueous Environment

    Energy Technology Data Exchange (ETDEWEB)

    Wire, G. L.; Mills, W. J.

    2002-08-01

    Fatigue crack propagation (FCP) rates for 304 stainless steel (304SS) were determined in 24 degree C and 288 degree C air and 288 degree C water using double-edged notch (DEN) specimens of 304 stainless steel (304 SS). Test performed at matched loading conditions in air and water at 288 degree C with 20-6- cc h[sub]2/kg h[sub]2O provided a direct comparison of the relative crack growth rates in air and water over a wide range of crack growth rates. The DEN crack extension ranged from short cracks (0.03-0.25 mm) to long cracks up to 4.06 mm, which are consistent with conventional deep crack tests. Crack growth rates of 304 SS in water were about 12 times the air rate. This 12X environmental enhancement persisted to crack extensions up to 4.06 mm, far outside the range associated with short crack effects. The large environmental degradation for 304 SS crack growth is consistent with the strong reduction of fatigue life in high hydrogen water. Further, very similar environmental effects w ere reported in fatigue crack growth tests in hydrogen water chemistry (HWC). Most literature data in high hydrogen water show only a mild environmental effect for 304 SS, of order 2.5 times air or less, but the tests were predominantly performed at high cyclic stress intensity or equivalently, high air rates. The environmental effect in low oxygen environments at low stress intensity depends strongly on both the stress ratio, R, and the load rise time, T[sub]r, as recently reported for austenitic stainless steel in BWR water. Fractography was performed for both tests in air and water. At 288 degree C in water, the fracture surfaces were crisply faceted with a crystallographic appearance, and showed striations under high magnification. The cleavage-like facets on the fracture surfaces suggest that hydrogen embrittlement is the primary cause of accelerated cracking.

  20. Water and CO₂ permeability of SsAqpZ, the cyanobacterium Synechococcus sp. PCC7942 aquaporin.

    Science.gov (United States)

    Ding, Xiaodong; Matsumoto, Tadashi; Gena, Patrizia; Liu, Chengwei; Pellegrini-Calace, Marialuisa; Zhong, Shihua; Sun, Xiaoli; Zhu, Yanming; Katsuhara, Maki; Iwasaki, Ikuko; Kitagawa, Yoshichika; Calamita, Giuseppe

    2013-03-01

    Cyanobacteria possess Aquaporin-Z (AqpZ) membrane channels which have been suggested to mediate the water efflux underlying osmostress-inducible gene expression and to be essential for glucose metabolism under photomixotrophic growth. However, preliminary observations suggest that the biophy-sical properties of transport and physiological meaning of AqpZ in such photosynthetic microorganisms are not yet completely assessed. In this study, we used Xenopus laevis oocyte and proteoliposome systems to directly demonstrate the water permeability of the cyanobacterium Synechococcus sp. PCC7942 aquaporin, SsAqpZ. By an in vitro assay of intracellular acidification in yeast cells, SsAqpZ was found to transport also CO2 . Consistent with this result, during the entire exponential phase of growth, Synechococcus SsAqpZ-null-mutant cells grew slower than the corresponding wild-type cells. This phenotype was stronger with higher levels of extracellular CO2 . In line with the conversion of CO2 gas into HCO3(-) ions under alkaline conditions, the impairment in growth of the SsAqpZ-null strain was weaker in more alkaline culture medium. Cyanobacterial SsAqpZ may exert a pleiotropic function in addition to the already reported roles in macronutrient homeostasis and osmotic-stress response as it appears to constitute an important pathway in CO2 uptake, a fundamental step in photosynthesis. Copyright © 2013 Soçiété Française des Microscopies and Soçiété de Biologie Cellulaire de France.

  1. MADS-box transcription factor SsMADS is involved in regulating growth and virulence in Sclerotinia sclerotiorum.

    Science.gov (United States)

    Qu, Xiaoyan; Yu, Baodong; Liu, Jinliang; Zhang, Xianghui; Li, Guihua; Zhang, Dongjing; Li, Le; Wang, Xueliang; Wang, Lu; Chen, Jingyuan; Mu, Wenhui; Pan, Hongyu; Zhang, Yanhua

    2014-05-08

    MADS-box proteins, a well-conserved family of transcription factors in eukaryotic organisms, specifically regulate a wide range of cellular functions, including primary metabolism, cell cycle, and cell identity. However, little is known about roles of the MADS-box protein family in the fungal pathogen Sclerotinia sclerotiorum. In this research, the S. sclerotiorum MADS-box gene SsMADS was cloned; it encodes a protein that is highly similar to Mcm1 orthologs from Saccharomyces cerevisiae and other fungi, and includes a highly conserved DNA-binding domain. MADS is a member of the MADS box protein SRF (serum response factor) lineage. SsMADS function was investigated using RNA interference. Silenced strains were obtained using genetic transformation of the RNA interference vectors pS1-SsMADS and pSD-SsMADS. SsMADS expression levels in silenced strains were analyzed using RT-PCR. The results showed that SsMADS mRNA expression in these silenced strains was reduced to different degrees, and growth rate in these silenced strains was significantly decreased. Infecting tomato leaflets with silenced strains indicated that SsMADS was required for leaf pathogenesis in a susceptible host. Our results suggest that the MADS-box transcription factor SsMADS is involved in S. sclerotiorum growth and virulence.

  2. Draft genome sequence of Streptomyces sp. strain SS, which produces a series of uridyl peptide antibiotic sansanmycins.

    Science.gov (United States)

    Wang, Lifei; Xie, Yunying; Li, Qinglian; He, Ning; Yao, Entai; Xu, Hongzhang; Yu, Ying; Chen, Ruxian; Hong, Bin

    2012-12-01

    Streptomyces sp. SS produces a series of uridyl peptide antibiotic sansanmycins. Here, we present a draft genome sequence of Streptomyces sp. SS containing the biosynthetic gene cluster for the antibiotics. The identification of the biosynthetic gene cluster of sansanmycins may provide further insight into biosynthetic mechanisms for uridyl peptide antibiotics.

  3. ss-siRNAs allele selectively inhibit ataxin-3 expression: multiple mechanisms for an alternative gene silencing strategy.

    Science.gov (United States)

    Liu, Jing; Yu, Dongbo; Aiba, Yuichiro; Pendergraff, Hannah; Swayze, Eric E; Lima, Walt F; Hu, Jiaxin; Prakash, Thazha P; Corey, David R

    2013-11-01

    Single-stranded silencing RNAs (ss-siRNAs) provide an alternative approach to gene silencing. ss-siRNAs combine the simplicity and favorable biodistribution of antisense oligonucleotides with robust silencing through RNA interference (RNAi). Previous studies reported potent and allele-selective inhibition of human huntingtin expression by ss-siRNAs that target the expanded CAG repeats within the mutant allele. Mutant ataxin-3, the genetic cause of Machado-Joseph Disease, also contains an expanded CAG repeat. We demonstrate here that ss-siRNAs are allele-selective inhibitors of ataxin-3 expression and then redesign ss-siRNAs to optimize their selectivity. We find that both RNAi-related and non-RNAi-related mechanisms affect gene expression by either blocking translation or affecting alternative splicing. These results have four broad implications: (i) ss-siRNAs will not always behave similarly to analogous RNA duplexes; (ii) the sequences surrounding CAG repeats affect allele-selectivity of anti-CAG oligonucleotides; (iii) ss-siRNAs can function through multiple mechanisms and; and (iv) it is possible to use chemical modification to optimize ss-siRNA properties and improve their potential for drug discovery.

  4. Microbiologic methods utilized in the MAL-ED cohort study.

    Science.gov (United States)

    Houpt, Eric; Gratz, Jean; Kosek, Margaret; Zaidi, Anita K M; Qureshi, Shahida; Kang, Gagandeep; Babji, Sudhir; Mason, Carl; Bodhidatta, Ladaporn; Samie, Amidou; Bessong, Pascal; Barrett, Leah; Lima, Aldo; Havt, Alexandre; Haque, Rashidul; Mondal, Dinesh; Taniuchi, Mami; Stroup, Suzanne; McGrath, Monica; Lang, Dennis

    2014-11-01

    A central hypothesis of The Etiology, Risk Factors and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development (MAL-ED) study is that enteropathogens contribute to growth faltering. To examine this question, the MAL-ED network of investigators set out to achieve 3 goals: (1) develop harmonized protocols to test for a diverse range of enteropathogens, (2) provide quality-assured and comparable results from 8 global sites, and (3) achieve maximum laboratory throughput and minimum cost. This paper describes the rationale for the microbiologic assays chosen and methodologies used to accomplish the 3 goals. © The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  5. Safe Handling of Snakes in an ED Setting.

    Science.gov (United States)

    Cockrell, Melanie; Swanson, Kristofer; Sanders, April; Prater, Samuel; von Wenckstern, Toni; Mick, JoAnn

    2017-01-01

    Efforts to improve consistency in management of snakes and venomous snake bites in the emergency department (ED) can improve patient and staff safety and outcomes, as well as improve surveillance data accuracy. The emergency department at a large academic medical center identified an opportunity to implement a standardized process for snake disposal and identification to reduce staff risk exposure to snake venom from snakes patients brought with them to the ED. A local snake consultation vendor and zoo Herpetologist assisted with development of a process for snake identification and disposal. All snakes have been identified and securely disposed of using the newly implemented process and no safety incidents have been reported. Other emergency department settings may consider developing a standardized process for snake disposal using listed specialized consultants combined with local resources and suppliers to promote employee and patient safety. Copyright © 2017 Emergency Nurses Association. Published by Elsevier Inc. All rights reserved.

  6. Prepared for the unthinkable: EDs respond to terrorist attacks.

    Science.gov (United States)

    2001-11-01

    The response to the terrorist attacks at the World Trade Center and the Pentagon provided many key lessons for disaster preparedness. Volunteer physicians were not used in most cases, but EDs recognized the need for a system to check credentials. Communication from disater sites was problem. Injured patients went to the nearest hospital, even if it was not the most appropriate hospital. Experts recommend using existing disaster plans as templates to design or update your own.

  7. MobilED: a step backwards to look ahead

    CSIR Research Space (South Africa)

    Van Den Berg, M

    2008-10-01

    Full Text Available of technology development and the widespread integration of the device in social and cultural practices (Kress & Pachler, 2007), has created unique opportunities for educators, researchers and practitioners. Mobile learning has developed from a few small scale... only ever reach a small fraction of them, while the network of invisible computers continues its exponential penetration into those same regions, below the radar(Selanikio, 2008). MobilED (Mobile Education) (2005) started as an international...

  8. Interact-ed interwinning nature of virtual reality

    OpenAIRE

    Özenç, Kürşat Fatih; Ozenc, Kursat Fatih

    2004-01-01

    In our highly computerized world, we live in an environment which resembles an intertwining mobius of 'real' and 'virtual'. In interact-ed's visual context, the virtual and the real have been quettioned with respect to this interrelation. The investigation is moreover done with the concept of dreams. Dreams, like virtual environments, affect our life 'apperently'; not 'really'. As affective experiences, virtual (environments) and dreams are tightly related to each other. In somne conditions t...

  9. Development of Ss-NIE-1 recombinant antigen based assays for immunodiagnosis of strongyloidiasis.

    Directory of Open Access Journals (Sweden)

    Lisa N Rascoe

    2015-04-01

    Full Text Available Strongyloides stercoralis is a widely distributed parasite that infects 30 to 100 million people worldwide. In the United States strongyloidiasis is recognized as an important infection in immigrants and refugees. Public health and commercial reference laboratories need a simple and reliable method for diagnosis of strongyloidiasis to identify and treat cases and to prevent transmission. The recognized laboratory test of choice for diagnosis of strongyloidiasis is detection of disease specific antibodies, most commonly using a crude parasite extract for detection of IgG antibodies. Recently, a luciferase tagged recombinant protein of S. stercoralis, Ss-NIE-1, has been used in a luciferase immunoprecipitation system (LIPS to detect IgG and IgG4 specific antibodies. To promote wider adoption of immunoassays for strongyloidiasis, we used the Ss-NIE-1 recombinant antigen without the luciferase tag and developed ELISA and fluorescent bead (Luminex assays to detect S. stercoralis specific IgG4. We evaluated the assays using well-characterized sera from persons with or without presumed strongyloidiasis. The sensitivity and specificity of Ss-NIE-1 IgG4 ELISA were 95% and 93%, respectively. For the IgG4 Luminex assay, the sensitivity and specificity were 93% and 95%, respectively. Specific IgG4 antibody decreased after treatment in a manner that was similar to the decrease of specific IgG measured in the crude IgG ELISA. The sensitivities of the Ss-NIE-1 IgG4 ELISA and Luminex assays were comparable to the crude IgG ELISA but with improved specificities. However, the Ss-NIE-1 based assays are not dependent on native parasite materials and can be performed using widely available laboratory equipment. In conclusion, these newly developed Ss-NIE-1 based immunoassays can be readily adopted by public health and commercial reference laboratories for routine screening and clinical diagnosis of S. stercoralis infection in refugees and immigrants in the United

  10. Active Intervention Can Decrease Burnout In Ed Nurses.

    Science.gov (United States)

    Wei, Rong; Ji, Hong; Li, Jianxin; Zhang, Liyao

    2017-03-01

    The aim of this study was to evaluate whether active intervention can decrease job burnout and improve performance among ED nurses. This study was carried out in the emergency departments of 3 hospitals randomly selected from 8 comprehensive high-level hospitals in Jinan, China. A total of 102 nurses were enrolled and randomly divided into control and intervention groups. For 6 months, nurses in intervention groups were treated with ordinary treatment plus comprehensive management, whereas nurses in the control group were treated with ordinary management, respectively. Questionnaires were sent and collected at baseline and at the end of the study. The Student t test was used to evaluate the effect of comprehensive management in decreasing burnout. All ED nurses showed symptoms of job burnout at different levels. Our data indicated that comprehensive management significantly decreased emotional exhaustion and depersonalization (P burnout in ED nurses and contribute to relieving work-related stress and may further protect against potential mental health problems. Copyright © 2016 Emergency Nurses Association. Published by Elsevier Inc. All rights reserved.

  11. Teachers are students in ZPG program called 'Pop Ed.'.

    Science.gov (United States)

    Schline, S

    1977-01-01

    Zero Population Growth's Population Education (Pop Ed) program began in 1975 as an ongoing effort to bring the "real world" into the classroom by demonstrating the relationships among population trends, food and energy resources, and environmental and economic problems. The training workshops which last for a day or 2 have the following goals: 1) to offer a brief demographic overview for teachers, 2) to provide lesson plans and techniques readily usable in the classroom, 3) to alert teachers to the best written and audiovisual materials available, 4) to identify local resources for teaching Pop Ed, and 5) to provide sample materials. In the 1st year of program operation 10 workshops were held. These workshops are credited, at least partly, with the subsequent population instruction that reached over 10,400 students and 1600 teachers. Another 15 workshops were held in the 2nd year of operation. Obstacles to the program are the assumption on the part of some teachers that Pop Ed belongs in disciplines other than their own and the belief on the part of many teachers that they will require extensive training.

  12. States leverage telepsychiatry solutions to ease ED crowding, accelerate care.

    Science.gov (United States)

    2015-02-01

    Many states are having success turning to telepsychiatry-based solutions to connect mental health patients with needed care while also decompressing crowded EDs. Just one year into a statewide telepsychiatry initiative in North Carolina (NC-STeP), administrators say the approach has saved as much as $7 million, and hospital demand for the service is higher than anticipated. In Texas, mental health emergency centers (MHEC) that use telepsychiatry to connect patients in rural areas with needed psychiatric care are freeing up EDs to focus on medical care. In just 11 months, 91 North Carolina hospitals have at least started the process to engage in NC-STeP. Much of the savings from NC-STeP come from involuntary commitment orders being overturned as a result of the telepsychiatry consults, reducing the need for expensive inpatient care. Implementing NC-STeP has involved multiple hurdles including credentialing difficulties and technical/firewall challenges. The Texas model provides 24/7 availability of psychiatrists via telemedicine through a network of MHECs. In-person staff at the MHECs perform basic screening tests and blood draws so that medical clearance can be achieved without the need for an ED visit in most cases. Funding for the MHECs comes from the state, hospitals in the region, and local governmental authorities that reap savings or benefits from the initiative.

  13. s ss S = S SS

    African Journals Online (AJOL)

    Dr Ahmed

    In all these cases, sorting may be used to achieve the arrangement in order of magnitude. The applications of sorting algorithm are numerous and cannot be exhausted in this paper. An efficient algorithm will provide better sorting time and easy of implementation. We described some popular sorting algorithm of interest, the ...

  14. Analysis of microtraces in invasive traumas using SEM/EDS.

    Science.gov (United States)

    Vermeij, E J; Zoon, P D; Chang, S B C G; Keereweer, I; Pieterman, R; Gerretsen, R R R

    2012-01-10

    Scanning electron microscopy in combination with energy-dispersive X-ray spectrometry (SEM/EDS) is a proven forensic tool and has been used to analyze several kinds of trace evidence. A forensic application of SEM/EDS is the examination of morphological characteristics of tool marks that tools and instruments leave on bone. The microtraces that are left behind by these tools and instruments on the bone are, however, often ignored or not noticed at all. In this paper we will describe the use of SEM/EDS for the analysis of microtraces in invasive sharp-force, blunt-force and bone-hacking traumas in bone. This research is part of a larger multi-disciplinary approach in which pathologists, forensic anthropologists, toolmark and microtrace experts work together to link observed injuries to a suspected weapon or, in case of an unknown weapon, to indicate a group of objects that could have been used as a weapon. Although there are a few difficulties one have to consider, the method itself is rather simple and straightforward to apply. A sample of dry and clean bone is placed into the SEM sample chamber and brightness and contrast are set such that bone appears grey, metal appears white and organic material appears black. The sample is then searched manually to find relevant features. Once features are found their elemental composition is measured by an energy dispersive X-ray spectrometer (EDS). This method is illustrated using several cases. It is shown that SEM/EDS analysis of microtraces in bone is a valuable tool to get clues about an unknown weapon and can associate a specific weapon with injuries on the basis of appearance and elemental composition. In particular the separate results from the various disciplines are complementary and may be combined to reach a conclusion with a stronger probative value. This is not only useful in the courtroom but above all in criminal investigations when one have to know for what weapon or object to look for. Copyright © 2011

  15. Health care utilization before and after an outpatient ED visit in older people.

    Science.gov (United States)

    Horney, Carolyn; Schmader, Kenneth; Sanders, Linda L; Heflin, Mitchell; Ragsdale, Luna; McConnell, Eleanor; Hocker, Michael; Hastings, S Nicole

    2012-01-01

    Older adults in the United States receive a significant amount of care in the emergency department (ED), yet the associations between ED and other types of health care utilization have not been adequately studied in this population. The goals of this study were to examine the relationships between health care use before and after an ED visit among older adults. This retrospective cohort study examined health care use among 308 patients 65 years or older discharged from a university-affiliated ED. Proportional-hazards models were used to assess the relationship between pre-ED health care use (primary care physician [PCP], specialist, ED, and hospital) and risk of return ED visits. Older ED patients in this study had visited other types of providers frequently in the previous year (median number of PCP and specialist visits, 4). Patients who used the ED on 2 or more occasions in the previous year were found to have visited their PCP more often than those without frequent ED use (median number of visits, 7.0 vs 4.0; P < .001). Despite more PCP use in this population, frequent ED use was associated with increased risk of a repeat ED visit (hazard ratio, 2.20; 95% confidence interval, 1.15-4.21), in models adjusted for demographics and health status. Older adults who use the ED are also receiving significant amounts of care from other sources; simply providing additional access to care may not improve outcomes for these vulnerable individuals. Published by Elsevier Inc.

  16. Ruokataukojen vaikutus työssäjaksamiseen ravintola-alalla Kuopiossa

    OpenAIRE

    Niemi, Roosa-Maria; Tuomainen, Teija

    2015-01-01

    Tämän opinnäytetyön tarkoitus on tutkia, kuinka ravintoloiden henkilökunta viettää taukoja ja kuinka ravinto vaikuttaa työssäjaksamiseen. Laki sekä työehdot määräävät työajoista ja tauotuksesta, mutta niiden toteutuminen käytännössä ei välttämättä mene lakien mukaan. Opinnäytetyön teoreettisessa osuudessa käsittelemme työlainsäädäntöä, ravitsemusta sekä työhyvinvointia. Ker-romme tarkemmin Matkailu-, ravintola- ja vapaa-ajan palveluiden työehtosopimuksesta sekä työaikoihin liittyvistä la...

  17. Bioconversion of glycerol for bioethanol production using isolated Escherichia coli SS1

    Directory of Open Access Journals (Sweden)

    Sheril Norliana Suhaimi

    2012-06-01

    Full Text Available Bioconverting glycerol into various valuable products is one of glycerol's promising applications due to its high availability at low cost and the existence of many glycerol-utilizing microorganisms. Bioethanol and biohydrogen, which are types of renewable fuels, are two examples of bioconverted products. The objectives of this study were to evaluate ethanol production from different media by local microorganism isolates and compare the ethanol fermentation profile of the selected strains to use of glucose or glycerol as sole carbon sources. The ethanol fermentations by six isolates were evaluated after a preliminary screening process. Strain named SS1 produced the highest ethanol yield of 1.0 mol: 1.0 mol glycerol and was identified as Escherichia coli SS1 Also, this isolated strain showed a higher affinity to glycerol than glucose for bioethanol production.

  18. CLaSS Computer Literacy Software: From Design to Implementation - A Three Year Student Evaluation

    Directory of Open Access Journals (Sweden)

    Ian Cole

    2006-12-01

    Full Text Available Both computer literacy and information retrieval techniques are required to undertake studies in higher education in the United Kingdom. This paper considers the research, development and the 3-year student evaluation of a piece of learning technology in computer and information literacy (CLaSS software. Students completed a questionnaire to examine their own assessment of knowledge and competence in computer and information literacy and based on this assessment CLaSS software was created to assist nursing students with computer and information literacy. This paper draws on existing literature and applies a specific learning model to the software while considering software engineering and user-centered design methodologies. The technical processes involved in designing and creating the software are briefly considered with software development data analysis discussed. A 3-year student evaluation of the software after it's release was undertaken to consider the long-term validity and usefulness of this software with the results analysed and discussed.

  19. EDS coal liquefaction process development. Phase V. EDS commercial plant study design update. Illinois coal. Volume 1. Main report

    Energy Technology Data Exchange (ETDEWEB)

    Epperly, W. R.

    1981-03-01

    The objectives of the Study Design Update (SDU) were to identify the technical issues facing a potential commercial-size EDS plant design; to provide a reliable basis for estimating the cost of EDS products; and to furnish research guidance to the EDS Project. The SDU consists of two distinct studies in which different processing schemes are used to produce the hydrogen and fuel gas required by the plant. These studies are referred to as the Base Case and the Market Flexibility Sensitivity Case. In the Base Case, hydrogen is generated by steam reforming of the light hydrocarbon gases produced in the plant. Fuel gas is generated by feeding the bottoms stream from the liquefaction section vacuum pipestill to a FLEXICOKING unit. In the FLEXICOKING unit reactor, the bottoms stream is converted to coke; additional liquid product is also recovered. The coke is converted to low-Btu fuel gas in the FLEXICOKING unit gasifier. In the Market Flexibility Sensitivity (MFS) Case, the bottoms stream from the vacuum pipestill is split, and about half is sent to the FLEXICOKING unit for recovery of additional liquid product and production of fuel gas. The remainder of the bottoms stream is converted to hydrogen in a Partial Oxidation Unit. Hence the MFS Case does not consume light hydrocarbon gases produced and they are available for sale. The study of these two cases has demonstrated the importance of bottoms process selection to the economics and thermal efficiency of an EDS plant. Volume 1 - Main Report has been developed to be a stand-alone document. Both the Base Case and Market Flexibility Sensitivity (MFS) Case are covered. This volume includes an overview and detailed case summaries. It also covers economics, product recovery factors, material and energy balances, cost estimates and enviromental considerations.

  20. Defining the Content of an Online Sexual Health Intervention: The MenSS Website

    OpenAIRE

    Webster, Rosie; Gerressu, Makeda; Michie, Susan; Estcourt, Claudia S; Anderson, Jane; Ang, Chee Siang; Murray, Elizabeth; Rait, Greta; Stephenson, Judith; Bailey, Julia V

    2015-01-01

    Background Health promotion and risk reduction are essential components of sexual health care. However, it can be difficult to prioritize these within busy clinical services. Digital interventions may provide a new method for supporting these. Objective The MenSS (Men?s Safer Sex) website is an interactive digital intervention developed by a multidisciplinary team, which aims to improve condom use in men who have sex with women (MSW). This paper describes the content of this intervention, and...

  1. Impact of the Arizona NExSS Winter School on Interdisciplinary Knowledge and Attitudes

    Science.gov (United States)

    Huff, Cierra; Burnam-Fink, Michael; Desch, Steven; Apai, Dániel

    2018-01-01

    The Nexus for Exoplanet System Science (NExSS) is a NASA-funded research coordination network whose focus is on investigating exoplanet diversity and devising strategies for searching for life on exoplanets. The fields of exoplanets and astrobiology are inherently highly interdisciplinary. Progress in these fields demands that researchers with various scientific backgrounds understand the issues and techniques of allied fields of study, including the tools and approaches used to solve different problems, as well as their limitations.In 2016, the NExSS teams at Arizona State University (ASU) and University of Arizona (UA) hosted 32 graduate students and postdoctoral researchers from various scientific backgrounds for one week at the Arizona NExSS Winter School. To bridge the gaps between fields and promote interdisciplinarity, students participated in lessons, field trips, hands-on activities, and a capstone proposal-writing activity. To assess the impact of the School on knowledge and attitudes about other fields, we administered a pre- and post-School questionnaire designed using the Impact Analysis Method of Davis & Scalice (2015).The results show that all participants gained knowledge at the School, especially in areas outside their primary field of study. The questionnaire revealed interesting differences in attitudes as well. When asked whether the geochemistry of Earth without life is predictable, planetary scientists were more likely than average to say yes, and geologists were more likely than average to say no. Their attitudes had converged after participation in the School. These results demonstrate that the Arizona NExSS Winter School was impactful not just in the knowledge gained, but in the interdisciplinary attitudes of students.

  2. KiSS-1 and GPR54 as new players in gonadotropin regulation and puberty.

    Science.gov (United States)

    Kaiser, Ursula B; Kuohung, Wendy

    2005-04-01

    The recent identification of loss-of-function mutations in the gene encoding GPR54, the receptor for the KiSS-1-derived peptides, kisspeptins, has highlighted a previously unrecognized pathway in the physiologic regulation of puberty and reproduction. Patients with loss-of-function mutations in GPR54 have idiopathic hypogonadotropic hypogonadism, and mice lacking GPR54 similarly fail to undergo puberty and have immature reproductive organs and low levels of sex steroids and gonadotropins. These observations have led to the hypothesis that kisspeptins activate hypothalamic GnRH release, thereby serving as a pivotal factor in the pubertal activation of the reproductive cascade. This hypothesis is supported by subsequent studies in rodent and primate models that have demonstrated localization of KiSS-1 mRNA in the hypothalamus, colocalization of GPR54 in GnRH neurons, GnRH-dependent activation of LH and FSH release by intracerebroventricular or peripheral administration of kisspeptin, and increased hypothalamic KiSS-1 and GPR54 mRNA levels at the onset of puberty. Taken together, these findings weave a compelling case for a role of the kisspeptin-GPR54 system in the activation of GnRH neurons at the time of pubertal awakening of the reproductive axis.

  3. GPR54 and KiSS-1: role in the regulation of puberty and reproduction.

    Science.gov (United States)

    Kuohung, Wendy; Kaiser, Ursula B

    2006-12-01

    The finding of inactivating mutations in GPR54 in IHH patients and the lack of reproductive maturation of the GPR54 null mouse have uncovered a previously unrecognized role for GPR54 and KiSS-1 in the physiologic regulation of puberty and reproduction. This newly identified function for GPR54 and its cognate ligand, kisspeptin, has led to additional studies that have localized GPR54 and KiSS-1 mRNA in the hypothalamus, colocalized GPR54 in GnRH neurons, demonstrated GnRH-dependent activation of LH and FSH release by kisspeptin, and shown increased hypothalamic KiSS-1 and GPR54 mRNA levels at the time of puberty. Taken together, these findings establish the role of the kisspeptin-GPR54 system in the stimulation of GnRH neurons during puberty. The mechanisms by which kisspeptin activates GnRH release, as well as the trigger for this pathway at the onset of puberty, are yet to be elucidated. In the future, modulators of GPR54 activity, including kisspeptin, may prove valuable in clinical applications in the fields of both cancer therapy and reproductive medicine.

  4. An MC-SS Platform for Short-Range Communications in the Personal Network Context

    Directory of Open Access Journals (Sweden)

    Dominique Noguet

    2008-03-01

    Full Text Available Wireless personal area networks (WPANs have gained interest in the last few years, and several air interfaces have been proposed to cover WPAN applications. A multicarrier spread spectrum (MC-SS air interface specified to achieve 130 Mbps in typical WPAN channels is presented in this paper. It operates in the 5.2 GHz ISM band and achieves a spectral efficiency of 3.25 b·s−1·Hz−1. Besides the robustness of the MC-SS approach, this air interface yields to reasonable implementation complexity. This paper focuses on the hardware design and prototype of this MC-SS air interface. The prototype includes RF, baseband, and IEEE802.15.3 compliant medium access control (MAC features. Implementation aspects are carefully analyzed for each part of the prototype, and key hardware design issues and solutions are presented. Hardware complexity and implementation loss are compared to theoretical expectations, as well as flexibility is discussed. Measurement results are provided for a real condition of operations.

  5. Biomolecular detection at ssDNA-conjugated nanoparticles by nano-impact electrochemistry.

    Science.gov (United States)

    Karimi, Anahita; Hayat, Akhtar; Andreescu, Silvana

    2017-01-15

    We describe the use of ssDNA functionalized silver nanoparticle (AgNP) probes for quantitative investigation of biorecognition and real time detection of biomolecular targets using nano-impact electrochemistry. The method is based on measurements of the individual collision events between ssDNA aptamer-functionalized AgNPs and a carbon fiber miroelectrode (CFME). Specific binding events of target analyte induced collision frequency changes enabling ultrasensitive detection of the aptamer target in a single step. These changes are assigned to the surface coverage of the NP by the ssDNA aptamers and subsequent conformational changes of the aptamer probe which affect the electron transfer between the NP and the electrode surface. The method enables sensitive and selective detection of ochratoxin A (OTA), chosen here as a model target, with a limit of detection of 0.05nM and a relative standard deviation of 4.9%. The study provides a means of characterizing bioconjugation of AgNPs with aptamers and assessing biomolecular recognition events with high sensitivity and without the use of exogenous reagents or enzyme amplification steps. This methodology can be broadly applicable to other bioconjugated systems, biosensing and related bioanalytical applications. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. Defining the Content of an Online Sexual Health Intervention: The MenSS Website.

    Science.gov (United States)

    Webster, Rosie; Gerressu, Makeda; Michie, Susan; Estcourt, Claudia; Anderson, Jane; Ang, Chee Siang; Murray, Elizabeth; Rait, Greta; Stephenson, Judith; Bailey, Julia V

    2015-07-03

    Health promotion and risk reduction are essential components of sexual health care. However, it can be difficult to prioritize these within busy clinical services. Digital interventions may provide a new method for supporting these. The MenSS (Men's Safer Sex) website is an interactive digital intervention developed by a multidisciplinary team, which aims to improve condom use in men who have sex with women (MSW). This paper describes the content of this intervention, and the rationale for it. Content was informed by a literature review regarding men's barriers to condom use, workshops with experts in sexual health and technology (N=16) and interviews with men in sexual health clinics (N=20). Data from these sources were analyzed thematically, and synthesized using the Behavior Change Wheel framework. The MenSS intervention is a website optimized for delivery via tablet computer within a clinic waiting room setting. Key targets identified were condom use skills, beliefs about pleasure and knowledge about risk. Content was developed using behavior change techniques, and interactive website features provided feedback tailored for individual users. This paper provides a detailed description of an evidence-based interactive digital intervention for sexual health, including how behavior change techniques were translated into practice within the design of the MenSS website. Triangulation between a targeted literature review, expert workshops, and interviews with men ensured that a range of potential influences on condom use were captured.

  7. Electropolymerization of camphorsulfonic acid doped conductive polypyrrole anti-corrosive coating for 304SS bipolar plates

    Science.gov (United States)

    Jiang, Li; Syed, Junaid Ali; Gao, Yangzhi; Zhang, Qiuxiang; Zhao, Junfeng; Lu, Hongbin; Meng, Xiangkang

    2017-12-01

    Conductive polymer coating doped with large molecular organic acid is an alternative method used to protect stainless steel (SS) bipolar plates in proton exchange membrane fuel cells (PEMFCs). However, it is difficult to select the proper doping acid, which improves the corrosion resistance of the coating without affecting its conductivity. In this study, large spatial molecular group camphorsulfonic acid (CSA) doped polypyrrole (PPY) conductive coating was prepared by galvanostatic electropolymerization on 304SS. The electrochemical properties of the coating were evaluated in 0.1 M H2SO4 solution in order to simulate the PEMFC service environment. The results indicate that the coating increased the corrosion potential and shifted Ecorr towards more positive value, particularly the jcorr value of PPY-CSA coated 304SS was dropped from 97.3 to 0.00187 μA cm-2. The long-term immersion tests (660 h) show that the PPY-CSA coating exhibits better corrosion resistance in comparison with the small acid (SO42-) doped PPY-SO42- or PPY/PPY-SO42- coatings. Moreover, the PPY-CSA coating presents low contact resistance and maintains strong corrosion resistance during the prolonged exposure time due to barrier effect and anodic protection.

  8. QoSS Hierarchical NoC-Based Architecture for MPSoC Dynamic Protection

    Directory of Open Access Journals (Sweden)

    Johanna Sepulveda

    2012-01-01

    Full Text Available As electronic systems are pervading our lives, MPSoC (multiprocessor system-on-chip security is becoming an important requirement. MPSoCs are able to support multiple applications on the same chip. The challenge is to provide MPSoC security that makes possible a trustworthy system that meets the performance and security requirements of all the applications. The network-on-chip (NoC can be used to efficiently incorporate security. Our work proposes the implementation of QoSS (quality of security service to overcome present MPSoC vulnerabilities. QoSS is a novel concept for data protection that introduces security as a dimension of QoS. QoSS takes advantage of the NoC wide system visibility and critical role in enabling system operation, exploiting the NoC components to detect and prevent a wide range of attacks. In this paper, we present the implementation of a layered dynamic security NoC architecture that integrates agile and dynamic security firewalls in order to detect attacks based on different security rules. We evaluate the effectiveness of our approach over several MPSoCs scenarios and estimate their impact on the overall performance. We show that our architecture can perform a fast detection of a wide range of attacks and a fast configuration of different security policies for several MPSoC applications.

  9. A novel, highly divergent ssDNA virus identified in Brazil infecting apple, pear and grapevine.

    Science.gov (United States)

    Basso, Marcos Fernando; da Silva, José Cleydson Ferreira; Fajardo, Thor Vinícius Martins; Fontes, Elizabeth Pacheco Batista; Zerbini, Francisco Murilo

    2015-12-02

    Fruit trees of temperate and tropical climates are of great economical importance worldwide and several viruses have been reported affecting their productivity and longevity. Fruit trees of different Brazilian regions displaying virus-like symptoms were evaluated for infection by circular DNA viruses. Seventy-four fruit trees were sampled and a novel, highly divergent, monopartite circular ssDNA virus was cloned from apple, pear and grapevine trees. Forty-five complete viral genomes were sequenced, with a size of approx. 3.4 kb and organized into five ORFs. Deduced amino acid sequences showed identities in the range of 38% with unclassified circular ssDNA viruses, nanoviruses and alphasatellites (putative Replication-associated protein, Rep), and begomo-, curto- and mastreviruses (putative coat protein, CP, and movement protein, MP). A large intergenic region contains a short palindromic sequence capable of forming a hairpin-like structure with the loop sequence TAGTATTAC, identical to the conserved nonanucleotide of circoviruses, nanoviruses and alphasatellites. Recombination events were not detected and phylogenetic analysis showed a relationship with circo-, nano- and geminiviruses. PCR confirmed the presence of this novel ssDNA virus in field plants. Infectivity tests using the cloned viral genome confirmed its ability to infect apple and pear tree seedlings, but not Nicotiana benthamiana. The name "Temperate fruit decay-associated virus" (TFDaV) is proposed for this novel virus. Copyright © 2015 Elsevier B.V. All rights reserved.

  10. Processing of A-form ssDNA by cryptic RNase H fold exonuclease PF2046.

    Science.gov (United States)

    Kim, Junsoo; Sambalkhundev, Gerelt-Od; Kim, Sulhee; Son, Jonghyeon; Han, Ah-Reum; Ko, Sul-Min; Hwang, Kwang Yeon; Lee, Woo Cheol

    2016-09-15

    RNase H fold protein PF2046 of Pyrococcus furiosus is a 3'-5' ssDNA exonuclease that cleaves after the second nucleotide from the 3' end of ssDNA and prefers poly-dT over poly-dA as a substrate. In our crystal structure of PF2046 complexed with an oligonucleotide of four thymidine nucleotides (dT4), PF2046 accommodates dT4 tightly in a groove and imposes steric hindrance on dT4 mainly by Phe220 such that dT4 assumes the A-form. As poly-dA prefer B-form due to the stereochemical restrictions, the A-form ssDNA binding by PF2046 should disfavor the processing of poly-dA. Phe220 variants display reduced activity toward poly-dA and the A-form appears to be a prerequisite for the processing by PF2046. Copyright © 2016 Elsevier Inc. All rights reserved.

  11. ssDNA degradation along capillary electrophoresis process using a Tris buffer.

    Science.gov (United States)

    Ric, Audrey; Ong-Meang, Varravaddheay; Poinsot, Verena; Martins-Froment, Nathalie; Chauvet, Fabien; Boutonnet, Audrey; Ginot, Frédéric; Ecochard, Vincent; Paquereau, Laurent; Couderc, François

    2017-06-01

    Tris-Acetate buffer is currently used in the selection and the characterization of ssDNA by capillary electrophoresis (CE). By applying high voltage, the migration of ionic species into the capillary generates a current that induces water electrolysis. This phenomenon is followed by the modification of the pH and the production of Tris derivatives. By injecting ten times by capillary electrophoresis ssDNA (50 nM), the whole oligonucleotide was degraded. In this paper, we will show that the Tris buffer in the running vials is modified along the electrophoretic process by electrochemical reactions. We also observed that the composition of the metal ions changes in the running buffer vials. This phenomenon, never described in CE, is important for fluorescent ssDNA analysis using Tris buffer. The oligonucleotides are degraded by electrochemically synthesized species (present in the running Tris vials) until it disappears, even if the separation buffer in the capillary is clean. To address these issues, we propose to use a sodium phosphate buffer that we demonstrate to be electrochemically inactive. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. Isolated positive anti-SS-B autoantibodies are not related to clinical features of systemic autoimmune diseases: Results from a routine population survey.

    Directory of Open Access Journals (Sweden)

    Sabine Jardel

    Full Text Available To assess in clinical practice the frequency and diagnosis associated with the SS-B-positive/SS-A negative autoantibody profile.We analyzed a one-year consecutive population of 624 patients referred by clinicians to the immunology laboratory to investigate anti-SS-A and/or anti-SS-B autoantibodies, who were detected using luminex technology. Data were analyzed for patients with isolated anti-SS-B autoantibodies. The clinical characteristics and diagnosis of connective tissue diseases (CTD were retrieved according to the international criteria.Among 1173 sera positive for anti-SS-A and/or anti-SS-B autoantibodies from 624 patients, we identified 84 patients (13.5% that had isolated anti-SS-B. Among the 75 patients positive for anti-SS-B with known clinical data, 15 were diagnosed with a CTD (20% including 4 systemic lupus erythematosus (5%, 4 rheumatoid arthritis (5%, 2 idiopathic inflammatory myositis (3%, 1 primary Sjögren's syndrome pSS (1%, 1 systemic sclerosis (1%, 2 undefined CTD (3%, and 1 mixed CTD (1%. Among the 60 other patients, 18 had non-CTD autoimmune diseases and 42 had non-autoimmune diseases. Within the CTD population, the presence of isolated anti-SS-B was not significantly associated to characteristic indicating a specific syndrome. There was no association between diagnosis of CTD and level of anti-SS-B autoantibodies (p = 0.70. Arthralgia was the more frequent sign and encountered in 10 patients (67%, of whom 3 had arthritis.The presence of anti-SS-B, without anti-SS-A autoantibodies using luminex technology, was not associated with CTD, especially pSS, in daily clinical practice. Our data suggests that the SS-B serological profile is not contributive for the classification criteria of pSS.

  13. Prevalence and correlates of erectile dysfunction (ED) and treatment seeking for ED in Asian Men: the Asian Men's Attitudes to Life Events and Sexuality (MALES) study.

    Science.gov (United States)

    Tan, Hui-Meng; Low, Wah Yun; Ng, Chirk Jenn; Chen, Kuang-Kuo; Sugita, Minoru; Ishii, Nobuhisa; Marumo, Ken; Lee, Sung Won; Fisher, William; Sand, Michael

    2007-11-01

    There have been limited multiregional studies in Asia examining the parameters of men's general and sexual health and quality of life in the general population vs. those in clinical cohorts of patients with erectile dysfunction (ED). The aims of the Asian Men's Attitudes to Life Events and Sexuality (Asian MALES) study were to investigate the prevalence of ED, associated health conditions, and ED treatment-seeking patterns in the general male population in five regions of Asia (China, Japan, Korea, Malaysia, and Taiwan). Standardized questionnaire previously used in a similar multiregional study and modified to ensure culturally appropriate content for Asia. Phase I of the study involved 10,934 adult men, aged 20-75 years, who were interviewed using the standardized questionnaire. Phase II of the study involved men with self-reported ED recruited from Phase I and via physician referral, invitations in general practitioner offices, and street interception (total Phase II sample, N = 1,209). The overall prevalence of self-reported ED in the Phase I study population was 6.4%. ED prevalence varied by region and significantly increased with age (P quality of life (P = 0.0001), compared with men without ED. Phase II of the study revealed that fewer than half of men with self-reported ED had sought treatment for their problem. Men were more likely to seek help for erection difficulties from Western doctors than from traditional medicine practitioners (P = 0.0001). A man's partner/spouse was the most common influencer of treatment seeking in all regions except Malaysia. The findings confirm those of existing research on ED in both Asian and non-Asian males: ED is a prevalent condition; the prevalence of ED increases with age and is strongly associated with comorbid conditions; and the majority of men have never sought treatment for their condition. This study highlights a substantial need for the evaluation and treatment of ED in Asian men.

  14. Treatment of Nausea and Vomiting in Pregnancy: Factors Associated with ED Revisits

    Directory of Open Access Journals (Sweden)

    Brian R. Sharp

    2016-09-01

    Full Text Available Introduction: Nausea and vomiting in pregnancy (NVP is a condition that commonly affects women in the first trimester of pregnancy. Despite frequently leading to emergency department (ED visits, little evidence exists to characterize the nature of ED visits or to guide its treatment in the ED. Our objectives were to evaluate the treatment of NVP in the ED and to identify factors that predict return visits to the ED for NVP. Methods: We conducted a retrospective database analysis using the electronic medical record from a single, large academic hospital. Demographic and treatment variables were collected using a chart review of 113 ED patient visits with a billing diagnosis of “nausea and vomiting in pregnancy” or “hyperemesis gravidarum.” Logistic regression analysis was used with a primary outcome of return visit to the ED for the same diagnoses. Results: There was wide treatment variability of nausea and vomiting in pregnancy patients in the ED. Of the 113 patient visits, 38 (33.6% had a return ED visit for NVP. High gravidity (OR 1.31, 95% CI [1.06-1.61], high parity (OR 1.50 95% CI [1.12-2.00], and early gestational age (OR 0.74 95% CI [0.60-0.90] were associated with an increase in return ED visits in univariate logistic regression models, while only early gestational age (OR 0.74 95% CI [0.59-0.91] was associated with increased return ED visits in a multiple regression model. Admission to the hospital was found to decrease the likelihood of return ED visits (p=0.002. Conclusion: NVP can be difficult to manage and has a high ED return visit rate. Optimizing care with aggressive, standardized treatment in the ED and upon discharge, particularly if factors predictive of return ED visits are present, may improve quality of care and reduce ED utilization for this condition.

  15. Patient-Centered Care Transition for Patients Admitted through the ED: Improving Patient and Employee Experience

    OpenAIRE

    Andrea Algauer BSN, RN; Stephanie Rivera BSN, RN; Robert Faurote BSN, RN

    2015-01-01

    With increasing wait times in emergency departments (ED) across America, there is a need to streamline the inpatient admission process in order to decrease wait times and more important, to increase patient and employee satisfaction. One inpatient unit at New York-Presbyterian Weill Cornell Medical Center initiated a program to help expedite the inpatient admission process from the ED. The goal of the ED Bridge program is to ease the patient's transition from the ED to an inpatient unit by vi...

  16. Ideologiasta kohti ideaalia : identiteetin ja maailmankäsityksen vaikutus teatteri-ilmaisun ohjaajan työssä

    OpenAIRE

    Aaltonen, Sanniveera

    2012-01-01

    Opinnäytetyössä tutkitaan ja reflektoidaan miten oma identiteetti ja henkilökohtainen maailmankäsitys vaikuttavat teatteri-ilmaisun ohjaajan työssä. Opinnäytetyössä esitellään autoetnografisen tutkimuksen kautta kirjoittajan subjektiivinen ja henkilökohtainen maailmankäsitys sekä identiteetin ilmentyminen suhteessa taiteelliseen työskentelyyn. Tutkimuksessa tarkastellaan identiteetin rakentumista ja eri osia postmodernin identiteettikäsityksen kautta. Käytännön esimerkkinä ja ensisijaise...

  17. Self-Esteem and Emotional Intelligence among B.Ed Trainees of Tsunami Affected Coastal Belt

    Science.gov (United States)

    Babu M, Sameer

    2008-01-01

    Through this study the author investigates the relationship between self-esteem and emotional intelligence among B.Ed trainees of Tsunami affected coastal belt of Alappey district of Kerala, India. Stream of study, marital status and age based comparisons were made among the B.Ed trainees. 92 B.Ed trainees were the participants in the study. It…

  18. EDS V26 Containment Vessel Explosive Qualification Test Report

    Energy Technology Data Exchange (ETDEWEB)

    Crocker, Robert W. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Haroldsen, Brent L. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Stofleth, Jerome H. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2013-11-01

    The objective of the test was to qualify the vessel for its intended use by subjecting it to a 1.25 times overtest. The criteria for success are that the measured strains do not exceed the calculated strains from the vessel analysis, there is no significant additional plastic strain on subsequent tests at the rated design load (shakedown), and there is no significant damage to the vessel and attached hardware that affect form, fit, or function. Testing of the V25 Vessel in 2011 established a precedent for testing V26 [2]. As with V25, two tests were performed to satisfy this objective. The first test used 9 pounds of Composition C-4 (11.25 lbs. TNT-equivalent), which is 125 percent of the design basis load. The second test used 7.2 pounds of Composition C-4 (9 lbs. TNT-equivalent) which is 100 percent of the design basis load. The first test provided the required overtest while the second test served to demonstrate shakedown and the absence of additional plastic deformation. Unlike the V25 vessel, which was mounted in a shipping cradle during testing, the V26 vessel was mounted on the EDS P2U3 trailer prior to testing. Visual inspections of the EDS vessel, surroundings, and diagnostics were completed before and after each test event. This visual inspection included analyzing the seals, fittings, and interior surfaces of the EDS vessel and documenting any abnormalities or damages. Photographs were used to visually document vessel conditions and findings before and after each test event.

  19. Book review: Psychology in a work context (2nd Ed.

    Directory of Open Access Journals (Sweden)

    Nanette Tredoux

    2003-10-01

    Full Text Available Bergh, Z. & Theron, A.L. (Eds (2003 Psychology in a work context (2nd Ed.. Cape Town: Oxford University Press. This book is an overview and introduction to Industrial and Organisational Psychology. It is a work of ambitious scope, and it is clear that the contributors have invested a great deal of thought and effort in the planning and execution of the book. The current version is the second edition, and it looks set to become one of those standard textbooks that are revised every few years to keep up with changing times. It is a handsome volume, produced to a high standard of editorial care, pleasingly laid out and organised well enough to be useful as an occasional reference source. An English-Afrikaans glossary, tables of contents for every chapter as well as for the entire book, a comprehensive index and extensive bibliography make it easy to retrieve the information relating to a particular topic. Every chapter ends with a conclusion summarising the gist of the material covered. Quality illustrations lighten the tone and help to bring some of the concepts to life. Learning outcomes and self-assessment exercises and questions for every chapter will be useful to the lecturer using the book as a source for a tutored course, and for the student studying by distance learning. If sold at the suggested retail price, the book represents good value compared to imported textbooks that cover similar ground.

  20. EMERGENCY DEPARTMENT (ED OVERCROWDING: EVIDENCE-BASED ANSWERS TO FREQUENTLY ASKED QUESTIONS

    Directory of Open Access Journals (Sweden)

    RJ Salway

    2017-03-01

    Full Text Available Overcrowding in emergency departments is a problem in many countries around the world, including the United States and Chile. Emergency department (ED overcrowding causes problems for patients and staff, including increased waiting times, increased ambulance diversion, increased length of stay, increased medical errors, increased patient mortality, and increased harm to hospitals due to financial losses. This article aims to describe the etiology of ED overcrowding and potential solutions through an examination of the evidence. Ultimately, ED overcrowding originates from hospital overcrowding and thus the solutions to this complex problem lie in the ED itself as well as outside of the ED.

  1. Improving ED specimen TAT using Lean Six Sigma.

    Science.gov (United States)

    Sanders, Janet H; Karr, Tedd

    2015-01-01

    Lean and Six Sigma are continuous improvement methodologies that have garnered international fame for improving manufacturing and service processes. Increasingly these methodologies are demonstrating their power to also improve healthcare processes. The purpose of this paper is to discuss a case study for the application of Lean and Six Sigma tools in the reduction of turnaround time (TAT) for Emergency Department (ED) specimens. This application of the scientific methodologies uncovered opportunities to improve the entire ED to lab system for the specimens. This case study provides details on the completion of a Lean Six Sigma project in a 1,000 bed tertiary care teaching hospital. Six Sigma's Define, Measure, Analyze, Improve, and Control methodology is very similar to good medical practice: first, relevant information is obtained and assembled; second, a careful and thorough diagnosis is completed; third, a treatment is proposed and implemented; and fourth, checks are made to determine if the treatment was effective. Lean's primary goal is to do more with less work and waste. The Lean methodology was used to identify and eliminate waste through rapid implementation of change. The initial focus of this project was the reduction of turn-around-times for ED specimens. However, the results led to better processes for both the internal and external customers of this and other processes. The project results included: a 50 percent decrease in vials used for testing, a 50 percent decrease in unused or extra specimens, a 90 percent decrease in ED specimens without orders, a 30 percent decrease in complete blood count analysis (CBCA) Median TAT, a 50 percent decrease in CBCA TAT Variation, a 10 percent decrease in Troponin TAT Variation, a 18.2 percent decrease in URPN TAT Variation, and a 2-5 minute decrease in ED registered nurses rainbow draw time. This case study demonstrated how the quantitative power of Six Sigma and the speed of Lean worked in harmony to improve

  2. Seasonal differential expression of KiSS-1/GPR54 in the striped hamsters (Cricetulus barabensis) among different tissues.

    Science.gov (United States)

    Xu, Laixiang; Xue, Huiliang; Li, Shenning; Xu, Jinhui; Chen, Lei

    2017-05-01

    Kisspeptins and G protein coupled receptor (GPR54) play significant roles in regulating reproductive activity among seasonally reproductive animals; however, the mechanisms of KiSS-1 and GPR54 gene affecting the seasonal reproduction in striped hamster are still unknown. In this study, real-time quantitative polymerase chain reaction was employed to examine the expression profiles of KiSS-1 and GPR54 in the hypothalamus, ovaries, testes, uterus and epididymis of striped hamsters across 4 different seasons. Our results showed that, across different seasons, the KiSS-1 expression mode of male striped hamsters and the GPR54 expression mode of female striped hamsters were consistent with the seasonal photoperiod in the hypothalamus. Meanwhile, across different seasons, the expression profile of KiSS-1 in the testes and the GPR54 expression profile of male striped hamsters in the hypothalamus were consistent with the intensity of their seasonal reproductive activity. Among different tissues, the expression trend for GPR54 is consistent across 4 seasons, while that for KiSS-1 is tissue-dependent. The expression trend for GPR54 across 4 seasons is the same regardless of gender, while that for KiSS-1 is dramatically different and sex-dependent across different seasons. These results suggest that the expressions of KiSS-1 and GPR54 in the striped hamsters were regulated by complicated mechanisms, and the regulatory mechanisms in the striped hamsters are seasonal-dependent and sex-dependent. This research will provide a theoretical basis for studying how KiSS-1 and GPR54 affect seasonal reproduction and the mechanisms behind their influence. © 2016 International Society of Zoological Sciences, Institute of Zoology/Chinese Academy of Sciences and John Wiley & Sons Australia, Ltd.

  3. Cross-cultural adaptation of the Chilean version of the Voice Symptom Scale - VoiSS.

    Science.gov (United States)

    Ruston, Francisco Contreras; Moreti, Felipe; Vivero, Martín; Malebran, Celina; Behlau, Mara

    This research aims to accomplish the cross-cultural equivalence of the Chilean version of the VoiSS protocol through its cultural and linguistic adaptation. After the translation of the VoiSS protocol to Chilean Spanish by two bilingual speech therapists and its back translation to English, we compared the items of the original tool with the previous translated version. The existing discrepancies were modified by a consensus committee of five speech therapists and the translated version was entitled Escala de Sintomas Vocales - ESV, with 30 questions and five answers: "Never", "Occasionally", "Sometimes", "Most of the time", "Always". For cross-cultural equivalence, the protocol was applied to 15 individuals with vocal problems. In each question the option of "Not applicable" was added to the answer choices for identification of the questions not comprehended or not appropriate for the target population. Two individuals had difficulty answering two questions, which made it necessary to adapt the translation of only one of them. The modified ESV was applied to three individuals with vocal problems, and there were incomprehensible inappropriate questions for the Chilean culture. The ESV reflects the original English version, both in the number of questions and the limitations of the emotional and physical domains. There is now a cross-cultural equivalence of VoiSS in Chilean Spanish, titled ESV. The validation of the ESV for Chilean Spanish is ongoing. RESUMEN Este estudio tuvo como objetivo realizar la equivalencia cultural de la versión Chilena del protocolo Voice Symptom Scale - VoiSS por medio de su adaptación cultural y lingüística. Después de la traducción del VoiSS para el Español Chileno, por dos fonoaudiólogos bilingües, y de la retro traducción para el inglés, se realizó una comparación de los ítems del instrumento original con la versión traducida, surgiendo discrepancias; tales divergencias fueron resueltas por un comité compuesto por

  4. Volume-dependent K+ transport in rabbit red blood cells comparison with oxygenated human SS cells

    Energy Technology Data Exchange (ETDEWEB)

    Al-Rohil, N.; Jennings, M.L.

    1989-07-01

    In this study the volume-dependent or N-ethylmaleimide (NEM)-stimulated, ouabain-insensitive K+ influx and efflux were measured with the tracer 86Rb+ in rabbit red blood cells. The purpose of the work was to examine the rabbit as a potential model for cell volume regulation in human SS red blood cells and also to investigate the relationship between the NEM-reactive sulfhydryl group(s) and the signal by which cell swelling activates the transport. Ouabain-resistant K+ efflux and influx increase nearly threefold in cells swollen hypotonically by 15%. Pretreatment with 2 mM NEM stimulates efflux 5-fold and influx 10-fold (each measured in an isotonic medium). The ouabain-resistant K+ efflux was dependent on the major anion in the medium. The anion dependence of K+ efflux in swollen or NEM-stimulated cells was as follows: Br- greater than Cl- much greater than NO3- = acetate. The magnitudes of both the swelling- and the NEM-stimulated fluxes are much higher in young cells (density separated but excluding reticulocytes) than in older cells. Swelling- or NEM-stimulated K+ efflux in rabbit red blood cells was inhibited 50% by 1 mM furosemide, and the inhibitory potency of furosemide was enhanced by extracellular K+, as is known to be true for human AA and low-K+ sheep red blood cells. The swelling-stimulated flux in both rabbit and human SS cells has a pH optimum at approximately 7.4. We conclude that rabbit red blood cells are a good model for swelling-stimulated K+ transport in human SS cells.

  5. [Rasch analysis on stroke-specific quality of life (SS-QOL) scale of acupuncture intervention on stroke].

    Science.gov (United States)

    Hui, Jian-Rong; Pei, Jian; Wang, Yuan-Chun; Hui, Jian-Ping; Fu, Qin-Hui; Song, Yi; Li, Hai-Yan; Liu, Zhi-Dan

    2013-04-01

    To optimize the evaluation level of active motor threshold and the functional domain of upper limbs of stroke-specific quality of life (SS-QOL) scale with Rasch analysis. Sixty patients with acute ischemic stroke that were in accord with research criterid were randomly divided into a test group (30 cases) and a control group (30 cases). Acupuncture treatment and routine western medicine were applied on the test group, and single treatment of routine western medicine was applied on the control group. Selected acupoints were MS 5, Fengchi (GB 20), Hegu (LI 4), etc. Active motor threshold and the functional domain of upper limbs of SS-QOL were self-tested by patients after one treating course. The characteristics of the above mentioned items were tested with Rasch model. The statistical result on fitness of active motor threshold and the functional domain of upper limbs of SS-QOL showed that every reference of samples and items wosin accord with the Rasch model and has well inner reliability and validity. The Infit and Outfit MnSq values of active motor threshold and the functional domain of upper limbs of SS-QOL are basically between 0.5 and 1.5. The application of Rasch analysis on the assessment of patient reported outcome (PRO) has optimized the PRO scale (the activity and upper limb function domain of SS-QOL scale) and enhanced evaluation level of active motor threshold and the functional domain of upper limbs of SS-QOL scale.

  6. A theoretical investigation on anomalous switching of single-stranded deoxyribonucleic acid (ssDNA) monolayers by water vapor

    Science.gov (United States)

    Zhao, Xin-Jun; Gao, Zhi-Fu; Jiang, Zhong-Ying

    2015-04-01

    In this paper, we use a molecular theory to study the anomalous switching of ssDNA monolayers. Here, both ssDNA-water and water-water hydrogen bonds and their explicit coupling to the ssDNA conformations are considered. We find that hydrogen bonding becomes a key element in inducing the anomalous switching of ssDNA monolayers. This finding accords well with the experimental observations. Based on our theoretical model, we predict that the anomalous switching induced by water vapor will be applicable to a wide range of hydrogen bonds polymers, and ssDNA-water hydrogen bonds and water-water hydrogen bonds hybridization will lead to the hydrogen-bond network formation of 3D ssDNA monolayers. Project supported by the National Natural Science Foundation of China (Grant Nos. 21264016, 11464047, and 21364016), the National Basic Research Program of China (Grant No. 2012CB821500), and the Natural Science Foundation of Xinjiang Uygur Autonomous Region, China (Grant No. 2013211A053).

  7. An Experimental Analysis to Reduce Cracks in Arc Welding in MS and SS 304 (L)

    OpenAIRE

    Vivekanand Jha; Mohd. Abbas

    2014-01-01

    cracks are very common welding defects .Cracks can be hot or cold cracks .Cracks can be reduced by various parameters keeping in mind. The various parameters are 1. Preheat 2.Interpass heating 3.Post weld heat treatment (PWHT) 4. Welding speed 5. Energy input 6. Thickness of joint 7. Stresses 8. High current 9. High carbon content. The aim of this experiment was to study different types of cracks formed during welding process and to reduce the cracking of SS 304 (L) and M.S in...

  8. Ikääntyneiden seksuaalisuus ja sen tukeminen hoitotyössä - ohjeita hoitajille

    OpenAIRE

    Hoffrén, Jenni; Eskelinen, Noora

    2014-01-01

    Tämän opinnäytetyön tarkoituksena on kuvata ikääntyneiden seksuaalisuutta ja kuinka hoitajat voivat tukea seksu-aalisuutta hoitotyössä. Opinnäytetyön tavoitteena on luoda opas ikääntyneiden seksuaalisuuden tukemiseksi Leivolan hoitohenkilökunnalle, johon kuuluu perus-, lähi- ja sairaanhoitajia. Toimeksiantajanamme on Leivolan palvelukeskus Leppävirralla, joka on ympärivuorokautinen hoitokoti. Palvelukeskuksen asukkaiden ikäjakauma on 63–99 vuotta. Opinnäytetyö on kehittämistyö. Opinnäyte...

  9. Corrosion Testing of 304L SS 3013 Inner Container and Teardrop Samples

    Energy Technology Data Exchange (ETDEWEB)

    Tokash, Justin Charles [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Hill, Mary Ann [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Lillard, Scott [Univ. of Akron, OH (United States); Joyce, Stephen Anthony [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Tegtmeier, Eric Lee [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Berg, John M. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Veirs, Douglas Kirk [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Worl, Laura Ann [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-06-27

    The Department of Energy (DOE) 3013 Standard specifies a minimum of two containers to be used for the storage of plutonium-bearing materials containing at least 30 wt.% plutonium and uranium. Three nested containers are typically used, the outer, inner, and convenience containers, shown in Figure 1. Both the outer and inner containers are sealed with a weld while the innermost convenience container must not be sealed. Lifetime of the containers is expected to be fifty years. The containers are fabricated of austenitic stainless steels (SS) due to their high corrosion resistance. Potential failure mechanisms of the storage containers have been examined by Kolman and Lillard et al.

  10. Imetys vauvaperhetyössä : opas sosiaalialalla toimiville vauvaperhetyöntekijöille

    OpenAIRE

    Nupponen, Anna

    2016-01-01

    Tämän opinnäytetyön tarkoituksena on ollut kirjoittaa opas imetyksestä sosiaalialalla toimiville vauvaperhetyöntekijöille. Sosiaalialalla imetys ei yleensä kuulu koulutukseen, vaikka vauvaperheiden kanssa työskentelevät saattavat tavata imettäviä äitejä usean kerran viikossa. Tässä kehittämishankkeessa kysyttiin lyhyen kyselylomakkeen avulla Helsingin kaupungin lastensuojelun perhekuntoutuksen Rastilan Vuorovaikutustiimin työntekijöiltä sekä Helsingin ensikodin työntekijöiltä heidän toive...

  11. Puun alkuperän hallinta Pyhtää Timber Ky:ssä

    OpenAIRE

    Kosonen, Kristian

    2012-01-01

    Puutuoteteollisuudessa erilaiset metsäsertifikaatit ja puun alkuperää todentavat Chain of Custody –sertifikaatit ovat yleistyneet viime vuosina ja tulevaisuudessa entistä pienemmät alan yritykset tulevat tarvitsemaan sertifikaatteja pysyäkseen markkinoilla ja kilpailussa mukana. Tämän työn tavoite on selvittää, miten Pyhtää Timber Ky voi saada FSC Chain of Custody -sertifikaatin. Työssä hahmotellaan puun alkuperän hallintajärjestelmä, jolle voidaan hakea sertifiointia. Työn alkuosassa ...

  12. Roche tomography of cataclysmic variables - VIII. The irradiated and spotted dwarf nova, SS Cygni

    Science.gov (United States)

    Hill, C. A.; Smith, Robert Connon; Hebb, L.; Szkody, P.

    2017-12-01

    We present the results of our spectroscopic study of the dwarf nova SS Cygni, using Roche tomography to map the stellar surface and derive the system parameters. Given that this technique takes into account the inhomogeneous brightness distribution on the surface of the secondary star, our derived parameters are (in principle) the most robust yet found for this system. Furthermore, our surface maps reveal that the secondary star is highly spotted, with strongly asymmetric irradiation on the inner hemisphere. Moreover, by constructing Doppler tomograms of several Balmer emission lines, we find strong asymmetric emission from the irradiated secondary star, and an asymmetric accretion disc that exhibits spiral structures.

  13. Tunti päivässä Facebook-yhteisön syntyminen

    OpenAIRE

    Sutinen, Sanna

    2011-01-01

    Opinnäytetyön tavoitteena oli toteuttaa Savonia-ammattikorkeakoulun Facebook-sivut. Työharjoittelussa tehtiin Savonialle selvitys sosiaalisen median käytöstä markkinoinnissa ja viestinnässä sekä sosiaalisen median toimintasuunnitelman vuodelle 2010. Selvityksessä Facebook nousi esiin yhtenä toteutettavana palveluna. Facebook on tunnettu, helppokäyttöinen ja sovelluksella on yli 500 miljoonaa käyttäjää. Visuaalisen ilmeen ja sisältöjen suunnittelu alkoi heti työharjoittelun päätyttyä syysku...

  14. Grüss type integral inequalities for generalized Riemann-Liouville k-fractional integrals

    Directory of Open Access Journals (Sweden)

    Shahid Mubeen

    2016-04-01

    Full Text Available Abstract Integral inequalities are considered to be important as they have many applications described by a number of researchers. Moreover, the theory of fractional calculus is used in solving differential, integral, and integro-differential equations and also in various other problems involving special functions. In this research article, we present the improved version of generalizations for a Grüss type integral inequality by taking a generalized Riemann-Liouville fractional integral in terms of a new parameter k > 0 $k>0$ . We contribute in the on going research by providing mathematical results that can be verified easily.

  15. A New Blind Adaptive Array Antenna Based on CMA Criteria for M-Ary/SS Signals Suitable for Software Defined Radio Architecture

    Science.gov (United States)

    Kozuma, Miho; Sasaki, Atsushi; Kamiya, Yukihiro; Fujii, Takeo; Umebayashi, Kenta; Suzuki, Yasuo

    M-ary/SS is a version of Direct Sequence/Spread Spectrum (DS/SS) aiming to improve the spectral efficiency employing orthogonal codes. However, due to the auto-correlation property of the orthogonal codes, it is impossible to detect the symbol timing by observing correlator outputs. Therefore, conventionally, a preamble has been inserted in M-ary/SS, signals. In this paper, we propose a new blind adaptive array antenna for M-ary/SS systems that combines signals over the space axis without any preambles. It is surely an innovative approach for M-ary/SS. The performance is investigated through computer simulations.

  16. Genetic studies of Ro (SS-A) and La (SS-B) autoantibodies in families with systemic lupus erythematosus and primary Sjögren's syndrome.

    Science.gov (United States)

    Arnett, F C; Hamilton, R G; Reveille, J D; Bias, W B; Harley, J B; Reichlin, M

    1989-04-01

    Using enzyme-linked immunosorbent assays, autoantibodies to Ro (SS-A) were detected in the sera of 21% of the first-degree relatives and 11% of the second-degree relatives of anti-Ro-positive probands with systemic lupus erythematosus (SLE) or primary Sjögren's syndrome, as compared with 3% of normal control subjects (P = 0.003 and P = 0.09, respectively). In a parallel study, anti-Ro occurred in 28% of the first-degree relatives of unselected members of families of SLE patients, regardless of the proband's serologic status, compared with 6% of the relatives from normal healthy families. Antibodies to La and to Sm/nuclear RNP were infrequent. Anti-Ro occurred in 41% of the relatives considered to have a dominant, non-HLA-linked "autoimmune trait" by virtue of having any autoimmune disorder and/or serologic abnormality (antinuclear antibodies, anti-single-stranded DNA, or biologic false-positive VDRL test result), as compared with only 2% of the healthy, seronegative relatives without the trait (P = 0.009). Moreover, HLA-DR2 and/or DR3 occurred in 90% of anti-Ro-positive subjects, regardless of their clinical status. These results demonstrate that the Ro autoantibody response occurs frequently in relatives of patients with SLE and is genetically mediated by both major histocompatibility complex and non-major histocompatibility complex effects.

  17. Fisica e fumetti: Paperone ed il deposito sotterraneo

    CERN Document Server

    Bagnoli, Franco

    2011-01-01

    I fumetti, come i film, spesso utilizzano idee scientifiche "fantasiose". Non ci riferiamo qui alla violazione implicita delle leggi della fisica, cosa permessa in un mondo di fantasia, quanto piuttosto all'uso di spiegazioni fisiche errate che vengono usate in buona fede perch\\'e a riflettono convinzioni molto diffuse, ma sbagliate, sull'interpretazione di fenomeni a partire dai principi fisici. D'altra parte questi errori possono servire a illustrare la corretta applicazione della fisica in una maniera molto pi\\`u accattivante rispetto alla modalit\\'a tradizionale di presentazione. Analizziamo qui l'avventura Paperone ed il deposito sotterraneo di Pezzin e Cavezzano. ----- Comics and cartoon movies sometimes exploit fictitious scientific ideas. It is often the case that these ideas, althought wrong, actually reflect the popular vision of some natural phenomenon. We do not refer here to the implicit violation of physical laws in fictions, a practice allowed by the underlining "poetic licence" of comics. Howe...

  18. Proteste ed emancipazione alla frontiera europea. Il caso di Lampedusa

    Directory of Open Access Journals (Sweden)

    Annalisa Lendaro

    Full Text Available Riassunto Questo articolo si propone di approfondire a livello teorico i legami tra proteste ed emancipazione dei migranti senza status legale, che si trovano in situazione di detenzione amministrativa sull’isola italiana di Lampedusa. Il presente contributo mette in evidenza il fatto che l’agency dei migranti, seppur privi di libertà e di uno status legale, è determinante per sbloccare una situazione di crisi in un contesto simile allo stato di eccezione descritto da Agamben. Più precisamente, la protesta dei migranti di Lampedusa mostra in che modo la sospensione dello stato di diritto apra degli spiragli per, non solo denunciare l’ingiustizia di alcune norme, ma anche per agire.

  19. EDS'09: 13th International Conference on Elastic & Diffractive Scattering

    CERN Multimedia

    CERN. Geneva

    2009-01-01

    The series of International Conferences on Elastic and Diffractive Scattering was founded in 1985 in the picturesque old French town of Blois, famous for its XIV - XVIIth century château, inside of which the first meeting took place. Since then, meetings have been organised every two years in different places of the world: New York (1987), Evanston (1989), Isola d'Elba (1991), Providence (1993), Blois (1995), Seoul (1997), Protvino (1999), Prague (2001), Helsinki (2003), Blois (2005) and Hamburg (2007). The conference will focus on the most recent experimental and theoretical results in particle physics with an emphasis on Quantum Chromodynamics (QCD). http://cern.ch/eds09/ The conference agenda is now full. No further contributions can be accepted.

  20. Effects of co-habitation between Anopheles gambiae s.s. and Culex quinquefasciatus aquatic stages on life history traits

    Directory of Open Access Journals (Sweden)

    Kweka Eliningaya J

    2012-02-01

    Full Text Available Abstract Background The effective measures for the control of malaria and filariasis vectors can be achieved by targeting immature stages of anopheline and culicine mosquitoes in productive habitat. To design this strategy, the mechanisms (like biotic interactions with conspecifc and heterospecific larvae regulating mosquito aquatic stages survivorship, development time and the size of emerging adults should be understood. This study explored the effect of co-habitation between An. gambiae s.s. and Cx. quinquefasciatus on different life history traits of both species under different densities and constant food supply in the habitats of the same size under semi-natural conditions. Methods Experiments were set up with three combinations; Cx. quinquefasciatus alone (single species treatment, An. gambiae s.s. alone (single species treatment; and An. gambiae s.s. with Cx. quiquefasciatus (co-habitation treatment in different densities in semi field situation. Results The effect of co-habitation of An. gambiae s.s. and Cx. quinquefasciatus was found to principally affect three parameters. The wing-lengths (a proxy measure of body size of An. gambiae s.s. in co-habitation treatments were significantly shorter in both females and males than in An. gambiae s.s single species treatments. In Cx. quinquefasciatus, no significant differences in wing-length were observed between the single species and co-habitation treatments. Daily survival rates were not significantly different between co-habitation and single species treatments for both An. gambiae s.s. and Cx. quinquefasciatus. Developmental time was found to be significantly different with single species treatments developing better than co-habitation treatments. Sex ratio was found to be significantly different from the proportion of 0.5 among single and co-habitation treatments species at different densities. Single species treatments had more males than females emerging while in co

  1. Effects of co-habitation between Anopheles gambiae s.s. and Culex quinquefasciatus aquatic stages on life history traits.

    Science.gov (United States)

    Kweka, Eliningaya J; Zhou, Goufa; Beilhe, Leila B; Dixit, Amruta; Afrane, Yaw; Gilbreath, Thomas M; Munga, Stephen; Nyindo, Mramba; Githeko, Andrew K; Yan, Guiyun

    2012-02-09

    The effective measures for the control of malaria and filariasis vectors can be achieved by targeting immature stages of anopheline and culicine mosquitoes in productive habitat. To design this strategy, the mechanisms (like biotic interactions with conspecifc and heterospecific larvae) regulating mosquito aquatic stages survivorship, development time and the size of emerging adults should be understood. This study explored the effect of co-habitation between An. gambiae s.s. and Cx. quinquefasciatus on different life history traits of both species under different densities and constant food supply in the habitats of the same size under semi-natural conditions. Experiments were set up with three combinations; Cx. quinquefasciatus alone (single species treatment), An. gambiae s.s. alone (single species treatment); and An. gambiae s.s. with Cx. quiquefasciatus (co-habitation treatment) in different densities in semi field situation. The effect of co-habitation of An. gambiae s.s. and Cx. quinquefasciatus was found to principally affect three parameters. The wing-lengths (a proxy measure of body size) of An. gambiae s.s. in co-habitation treatments were significantly shorter in both females and males than in An. gambiae s.s single species treatments. In Cx. quinquefasciatus, no significant differences in wing-length were observed between the single species and co-habitation treatments. Daily survival rates were not significantly different between co-habitation and single species treatments for both An. gambiae s.s. and Cx. quinquefasciatus. Developmental time was found to be significantly different with single species treatments developing better than co-habitation treatments. Sex ratio was found to be significantly different from the proportion of 0.5 among single and co-habitation treatments species at different densities. Single species treatments had more males than females emerging while in co-habitation treatments more females emerged than males. In this study

  2. Pivot Nursing: An Alternative to Traditional ED Triage.

    Science.gov (United States)

    Christensen, Maria; Rosenberg, Mark; Mahon, Eileen; Pineda, Sharon; Rojas, Eva; Soque, Victoria; Johansen, Mary L

    2016-09-01

    A 7.2% increase in patient volume from 130,700 to 140,800 in 2012 prompted St Joseph's Regional Medical Center Emergency Department to review existing triage processes to decrease turnaround time. "Pivot triage" is a new, efficient intake process that entails use of 4 rather than 8 determinants to identify acuity levels. The purpose of this performance improvement project was to create alternatives to traditional triage to decrease ED length of stay and door-to-physician time. After education, the pivot process was implemented using 4 determinants established by a multidisciplinary team. The pivot process was slowly implemented for 6 hours over a 1-week period to work out processing issues. Arrival time, door-to-physician time, and departure time from the emergency department were elements used to calculate the patient's turnaround time. Length of ED stay was collected monthly beginning in the fourth quarter of 2011. Comparisons were made after Pivot implementation in the fourth quarter of 2012. Despite the increasing volume, the mean door-to-physician time decreased from 71 to 40 minutes, a 43.7% reduction. The overall turnaround time decreased from 220 to 181 minutes, representing approximately a 17.7% reduction. The percentage of patients who left without being seen decreased from 2.5% to 1.0%. The pivot process improved patient flow in the emergency department, reducing time spent by the patient in the department. The pivot process is a viable alternative to traditional triage. Nurses are able to accurately pivot patients with a reduced amount of information. Copyright © 2016 Emergency Nurses Association. Published by Elsevier Inc. All rights reserved.

  3. ED services: the impact of caring behaviors on patient loyalty.

    Science.gov (United States)

    Liu, Sandra S; Franz, David; Allen, Monette; Chang, En-Chung; Janowiak, Dana; Mayne, Patricia; White, Ruth

    2010-09-01

    This article describes an observational study of caring behaviors in the emergency departments of 4 Ascension Health hospitals and the impact of these behaviors on patient loyalty to the associated hospital. These hospitals were diverse in size and geography, representing 3 large urban community hospitals in metropolitan areas and 1 in a midsized city. Research assistants from Purdue University (West Lafayette, IN) conducted observations at the first study site and validated survey instruments. The Purdue research assistants trained contracted observers at the subsequent study sites. The research assistants conducted observational studies of caregivers in the emergency departments at 4 study sites using convenience sampling of patients. Caring behaviors were rated from 0 (did not occur) to 5 (high intensity). The observation included additional information, for example, caregiver roles, timing, and type of visit. Observed and unobserved patients completed exit surveys that recorded patient responses to the likelihood-to-recommend (loyalty) questions, patient perceptions of care, and demographic information. Common themes across all study sites emerged, including (1) the area that patients considered most important to an ED experience (prompt attention to their needs upon arrival to the emergency department); (2) the area that patients rated as least positive in their actual ED experience (prompt attention to their needs upon arrival to the emergency department); (3) caring behaviors that significantly affected patient loyalty (eg, making sure that the patient is aware of care-related details, working with a caring touch, and making the treatment procedure clearly understood by the patient); and (4) the impact of wait time to see a caregiver on patient loyalty. A number of correlations between caring behaviors and patient loyalty were statistically significant (P caring behaviors that are most closely linked to patient loyalty but that occurred least frequently. The

  4. Caffeine inhibits gene conversion by displacing Rad51 from ssDNA.

    Science.gov (United States)

    Tsabar, Michael; Mason, Jennifer M; Chan, Yuen-Ling; Bishop, Douglas K; Haber, James E

    2015-08-18

    Efficient repair of chromosomal double-strand breaks (DSBs) by homologous recombination relies on the formation of a Rad51 recombinase filament that forms on single-stranded DNA (ssDNA) created at DSB ends. This filament facilitates the search for a homologous donor sequence and promotes strand invasion. Recently caffeine treatment has been shown to prevent gene targeting in mammalian cells by increasing non-productive Rad51 interactions between the DSB and random regions of the genome. Here we show that caffeine treatment prevents gene conversion in yeast, independently of its inhibition of the Mec1(ATR)/Tel1(ATM)-dependent DNA damage response or caffeine's inhibition of 5' to 3' resection of DSB ends. Caffeine treatment results in a dosage-dependent eviction of Rad51 from ssDNA. Gene conversion is impaired even at low concentrations of caffeine, where there is no discernible dismantling of the Rad51 filament. Loss of the Rad51 filament integrity is independent of Srs2's Rad51 filament dismantling activity or Rad51's ATPase activity and does not depend on non-specific Rad51 binding to undamaged double-stranded DNA. Caffeine treatment had similar effects on irradiated HeLa cells, promoting loss of previously assembled Rad51 foci. We conclude that caffeine treatment can disrupt gene conversion by disrupting Rad51 filaments. © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

  5. Ion Fluence and Energy Effects on the Optical Properties of SS Mirrors Bombarded by Hydrogen Ions

    Energy Technology Data Exchange (ETDEWEB)

    Bardamid, A.F. [T. Shevchenko National Univ., Kiev (Ukraine); Belyaeva, A.I. [National Technical Univ. KhPI, Kharkov (Ukraine); Bondarenko, V.N. [NSC KIPT, Kharkov (Ukraine)] (and others)

    2003-04-01

    The effects of hydrogen ion (H{sub 3}{sup +}) bombardment on the optical properties of polycrystal stainless steel (SS) mirrors were investigated. Ellipsometry, profilometry, scanning electron microscopy, angle distribution of scattered light, and spectral reflectance were used to characterize the surfaces. Results for the bombardment of SS mirrors with ions of fixed energy (0.3, 0.65 and 1.5 keV/H), with ion flux density (0.5-2) x 10{sup 20} H/m{sup 2}/s up to fluences of (1.1-4.3) x 10{sup 24} H/m{sup 2} are presented. The data show that the surface roughness rises with both increasing ion energy and ion fluence, and that surface roughening leads to an increase of the scattered light with a corresponding decrease of reflectance at normal incidence. The thickness of the apparent layer, obtained by ellipsometry, was found to rise with increasing ion energy at fixed ion fluence and with increasing ion fluence at fixed ion energy. Possible mechanisms for the ion energy effect on the degradation rate of stainless steel mirrors and the origin of the apparent layer are discussed.

  6. Corrosion Fatigue Behavior of 316LN SS in Acidified Sodium Chloride Solution at Applied Potential

    Science.gov (United States)

    Poonguzhali, A.; Pujar, M. G.; Mallika, C.; Mudali, U. Kamachi

    2015-05-01

    The influence of acidified 1 M NaCl solution by addition of 2 ml/L of HCl on the cyclic plastic deformation of AISI Type 316LN SS containing 0.07 wt.% and 0.22 wt.% N was investigated as a function of the applied potentials. The corrosion fatigue (CF) behavior of stainless steel (SS) was explained vis-a-vis the dislocation behavior, the propensity to form microcracks, and the evolution of the current transients based on the studies carried out at both room-temperature and boiling conditions. CF experiments were conducted using round tensile specimens at a stress ratio of 0.5 and a frequency of 0.1 Hz. Two different kinds of damage mechanisms were observed (I) the damage mechanism in the stable-passive state was correlated with the localization of the anodic dissolution due to a depassivation-repassivation process, whereas (II) the cyclic stress induced pitting corrosion in the metastable pitting state, which resulted in formation of microcracks. The study of the microcracking process and its evolution is a key to the physical mechanism by which the fatigue life of stainless steels would be affected in an aqueous corrosive solution under the applied potential.

  7. NExSS/NAI Joint ExoPAG SAG 16 Report on Remote Biosignatures for Exoplanets

    Science.gov (United States)

    Kiang, Nancy Y.; Parenteau, Mary Nicole; Domagal-Goldman, Shawn

    2017-01-01

    Future exoplanet observations will soon focus on the search for life beyond the Solar System. Exoplanet biosignatures to be sought are those with global, potentially detectable, impacts on a planet. Biosignatures occur in an environmental context in which geological, atmospheric, and stellar processes and interactions may work to enhance, suppress or mimic these biosignatures. Thus biosignature scienceis inherently interdisciplinary. Its advance is necessary to inform the design of the next flagship missions that will obtain spectra of habitable extrasolar planets. The NExSS NAI Joint Exoplanet Biosignatures Workshop Without Walls brought together the astrobiology, exoplanet, and mission concept communities to review, discuss, debate, and advance the science of remote detection of planetary biosignatures. The multi-meeting workshop began in June 2016, and was a process that engaged a broad range of experts across the interdisciplinary reaches of NASA's Nexus for Exoplanet System Science (NExSS) program, the NASA Astrobiology Institute (NAI), NASAs Exoplanet Exploration Program (ExEP), and international partners, such as the European Astrobiology Network Association (EANA) and Japans Earth Life Science Institute (ELSI). These groups spanned expertise in astronomy, planetary science, Earth sciences, heliophysics, biology, instrument mission development, and engineering.

  8. Hybrid nano-composites made of ss-DNA/wrapped carbon nanotubes and titania.

    Science.gov (United States)

    Romio, Martina; Mesa, Camillo La

    2017-04-01

    Multi-walled carbon nanotubes, MWCNTs, are stabilized thanks to the surface wrapping of single-strand DNA, ss-DNA; the resulting adducts are kinetically and thermodynamically stable Such entities build up nano-hybrids with titania, TiO2, nano-particles, in presence of surfactant as an adjuvant. The conditions leading to TiO2 adsorption onto ss-DNA/CNTs were investigated, by optimizing the concentration of adducts, nano-particles (NPs), and of the cationic surfactant (CTAB). Controlling the working conditions makes possible to get homogeneously organized hybrids. Characterization by DLS, electro-phoretic mobility, SEM and AFM clarified the surfactant-assisted association modes between adducts and CTAB-functionalized TiO2. Nano-particles' clustering onto DNA-wrapped adducts gives hybrids trough electrostatic interactions. Surface coverage by TiO2 is significant and homogeneous. It is expected that the reported hybrids can be useful for applications in heterogeneous catalysis. Copyright © 2016. Published by Elsevier B.V.

  9. Parameter Optimization Of Natural Hydroxyapatite/SS316l Via Metal Injection Molding (MIM)

    Science.gov (United States)

    Mustafa, N.; Ibrahim1, M. H. I.; Amin, A. M.; Asmawi, R.

    2017-01-01

    Metal injection molding (MIM) are well known as a worldwide application of powder injection molding (PIM) where as applied the shaping concept and the beneficial of plastic injection molding but develops the applications to various high performance metals and alloys, plus metal matrix composites and ceramics. This study investigates the strength of green part by using stainless steel 316L/ Natural hydroxyapatite composite as a feedstock. Stainless steel 316L (SS316L) was mixed with Natural hydroxyapatite (NHAP) by adding 40 wt. % Low Density Polyethylene and 60 %wt. Palm Stearin as a binder system at 63 wt. % powder loading consist of 90 % wt. of SS316 L and 10 wt. % NHAP prepared thru critical powder volume percentage (CPVC). Taguchi method was functional as a tool in determining the optimum green strength for Metal Injection Molding (MIM) parameters. The green strength was optimized with 4 significant injection parameter such as Injection temperature (A), Mold temperature (B), Pressure (C) and Speed (D) were selected throughout screening process. An orthogonal array of L9 (3)4 was conducted. The optimum injection parameters for highest green strength were established at A1, B2, C0 and D1 and where as calculated based on Signal to Noise Ratio.

  10. Varieties of charge distributions in coat proteins of ssRNA+  viruses

    Science.gov (United States)

    Lošdorfer Božič, Anže; Podgornik, Rudolf

    2018-01-01

    A major part of the interactions involved in the assembly and stability of icosahedral, positive-sense single-stranded RNA (ssRNA+) viruses is electrostatic in nature, as can be inferred from the strong pH- and salt-dependence of their assembly phase diagrams. Electrostatic interactions do not act only between the capsid coat proteins (CPs), but just as often provide a significant contribution to the interactions of the CPs with the genomic RNA, mediated to a large extent by positively charged, flexible N-terminal tails of the CPs. In this work, we provide two clear and complementary definitions of an N-terminal tail of a protein, and use them to extract the tail sequences of a large number of CPs of ssRNA+  viruses. We examine the pH-dependent interplay of charge on both tails and CPs alike, and show that—in contrast to the charge on the CPs—the net positive charge on the N-tails persists even to very basic pH values. In addition, we note a limit to the length of the wild-type genomes of those viruses which utilize positively charged tails, when compared to viruses without charged tails and similar capsid size. At the same time, we observe no clear connection between the charge on the N-tails and the genome lengths of the viruses included in our study.

  11. Ion Fluence and Energy Effects on the Optical Properties of SS Mirrors Bombarded by Hydrogen Ions

    Science.gov (United States)

    Bardamid, A. F.; Belyaeva, A. I.; Bondarenko, V. N.; Davis, J. W.; Galuza, A. A.; Garkusha, I. E.; Haasz, A. A.; Konovalov, V. G.; Kudlenko, A. D.; Poon, M.; Ryzhkov, I. V.; Solodovchenko, S. I.; Shtan, A. F.; Voitsenya, V. S.; Yakimov, K. I.

    The effects of hydrogen ion (H3+) bombardment on the optical properties of polycrystal stainless steel (SS) mirrors were investigated. Ellipsometry, profilometry, scanning electron microscopy, angle distribution of scattered light, and spectral reflectance were used to characterize the surfaces. Results for the bombardment of SS mirrors with ions of fixed energy (0.3, 0.65 and 1.5 keV/H), with ion flux density >(0.5-2) · 1020 H/m2s up to fluences of (1.1-4.3) · 1024 H}/m2 are presented.  The data show that the surface roughness rises with both increasing ion energy and ion fluence, and that surface roughening leads to an increase of the scattered light with a corresponding decrease of reflectance at normal incidence. The thickness of the apparent layer, obtained by ellipsometry, was found to rise with increasing ion energy at fixed ion fluence and with increasing ion fluence at fixed ion energy.  Possible mechanisms for the ion energy effect on the degradation rate of stainless steel mirrors and the origin of the apparent layer are discussed.

  12. Caffeine inhibits gene conversion by displacing Rad51 from ssDNA

    Science.gov (United States)

    Tsabar, Michael; Mason, Jennifer M.; Chan, Yuen-Ling; Bishop, Douglas K.; Haber, James E.

    2015-01-01

    Efficient repair of chromosomal double-strand breaks (DSBs) by homologous recombination relies on the formation of a Rad51 recombinase filament that forms on single-stranded DNA (ssDNA) created at DSB ends. This filament facilitates the search for a homologous donor sequence and promotes strand invasion. Recently caffeine treatment has been shown to prevent gene targeting in mammalian cells by increasing non-productive Rad51 interactions between the DSB and random regions of the genome. Here we show that caffeine treatment prevents gene conversion in yeast, independently of its inhibition of the Mec1ATR/Tel1ATM-dependent DNA damage response or caffeine's inhibition of 5′ to 3′ resection of DSB ends. Caffeine treatment results in a dosage-dependent eviction of Rad51 from ssDNA. Gene conversion is impaired even at low concentrations of caffeine, where there is no discernible dismantling of the Rad51 filament. Loss of the Rad51 filament integrity is independent of Srs2's Rad51 filament dismantling activity or Rad51's ATPase activity and does not depend on non-specific Rad51 binding to undamaged double-stranded DNA. Caffeine treatment had similar effects on irradiated HeLa cells, promoting loss of previously assembled Rad51 foci. We conclude that caffeine treatment can disrupt gene conversion by disrupting Rad51 filaments. PMID:26019181

  13. Effect of Microstructure on the Wear Behavior of Heat Treated SS-304 Stainless Steel

    Directory of Open Access Journals (Sweden)

    S. Kumar

    2016-12-01

    Full Text Available Sliding wear characteristics of some heat treated SS-304 stainless steel against EN-8 steel in dry condition have been studied in the present experimental work. Samples of SS-304 stainless steel have been heated in a muffle furnace in desired temperature and allowed to dwell for two hours. The heated specimen are then cooled in different media namely inside the furnace, open air, cutting grade oil (grade 44 and water at room temperature to obtain different grades of heat treatment. Microstructures and corresponding micro hardness of the samples have been measured along with Feritscopic studies. Wear characteristics have been studied in a multi tribo-tester (Ducom in dry sliding condition against EN-8 steel roller. Speed, load on job and duration of test run have been considered as the experimental parameters. The wear of the samples have been obtained directly from ‘Winducom 2006’ software. Mass loss of the samples before and after operation has also been considered as the measure of wear in the present study. All the samples have been slid against EN-8 steel roller with fixed experimental parameters. The data have been plotted, compared and analyzed. Effect of microstructures as well as micro hardness on the wear behavior has been studied and concluded accordingly.

  14. ssDNA Pairing Accuracy Increases When Abasic Sites Divide Nucleotides into Small Groups.

    Directory of Open Access Journals (Sweden)

    Alexandra Peacock-Villada

    Full Text Available Accurate sequence dependent pairing of single-stranded DNA (ssDNA molecules plays an important role in gene chips, DNA origami, and polymerase chain reactions. In many assays accurate pairing depends on mismatched sequences melting at lower temperatures than matched sequences; however, for sequences longer than ~10 nucleotides, single mismatches and correct matches have melting temperature differences of less than 3°C. We demonstrate that appropriately grouping of 35 bases in ssDNA using abasic sites increases the difference between the melting temperature of correct bases and the melting temperature of mismatched base pairings. Importantly, in the presence of appropriately spaced abasic sites mismatches near one end of a long dsDNA destabilize the annealing at the other end much more effectively than in systems without the abasic sites, suggesting that the dsDNA melts more uniformly in the presence of appropriately spaced abasic sites. In sum, the presence of appropriately spaced abasic sites allows temperature to more accurately discriminate correct base pairings from incorrect ones.

  15. Biosynthesis, characterization and antimicrobial activity of silver nanoparticles by Streptomyces sp. SS2.

    Science.gov (United States)

    Mohanta, Yugal Kishore; Behera, Sujit Kumar

    2014-11-01

    In the present study the microbial biosynthesis of silver nanoparticles (AgNPs) by secondary metabolites of Streptomyces sp. SS2 in an eco-friendly approach has been reported. The Streptomyces sp. SS2 was isolated from the soil sediment of Similipal Biosphere Reserve. The identification of this strain was determined by phenotypical characteristics (morphological and biochemical) and molecular characterization method using 16 s rDNA sequencing. The morphological study was also done by high-resolution scanning electron microscopy. The preliminary characterization of biosynthesized silver nanoparticle was carried out using UV-Vis spectrum analysis, which showed an absorption peak at 420 nm corresponding to plasmon absorption of silver. The average size and charge (zeta potential) of the particles were found to be 67.95 ± 18.52 nm and -17.7 ± 5.30 mV, respectively. The functional groups were identified by FTIR studies and their morphology (round and spherical shape) was determined by scanning electron microscopy. The synthesized AgNPs exhibited excellent antibacterial activity against Escherichia coli (MTCC 1089), Bacillus subtilis (MTCC 7164), Staphylococcus epidermis (MTCC 3615), Vibrio cholerae (MTCC 3904) and Staphylococcus aureus (MTCC 1144). These biotechnological approaches of synthesis of nanoparticles can direct a new path in biomaterial sciences and enrich biomedical applications.

  16. Lack of information underlies women's perceptions of erectile dysfunction (ED) drugs in Japan.

    Science.gov (United States)

    Mita, Koji; Kakehashi, Masayuki; Hasegawa, Yasuhisa; Inoue, Yoji; Shigeta, Masanobu; Matsubara, Akio; Usui, Tsuguru

    2008-12-01

    Some Japanese women are known to have negative attitudes toward erectile dysfunction (ED) drugs, but the environment underlying these perceptions is unclear. To reveal the underlying environment that shapes women's perceptions of ED drugs in Japan. A web-based questionnaire was conducted through an Internet-based market research company. A total of 2,593 women in five age groups (20s, 30s, 40s, 50s, and 60s or older) were randomly invited to participate in this study, with an almost equal number in each age group. The questionnaire contained 30 items related to individual background and sexual information, concerns about the image of ED drugs, ED drug-related perceptions, and attitude toward sexual information media. The women's attitude and the independent predictors that affect their partner's use of ED drugs were clarified. Answers were obtained from 1,077 women, of whom 35.4% (n=381) had a negative image of ED drugs. Although 69.5% (n=749) agreed that a sexual relationship with a male partner was important, only 26.7% (n=288) agreed that this remained important if ED drugs were used. However, 56.7% (n=611) and 57.7% (n=621) of respondents, respectively, answered that they would allow their partner's use of ED drugs if they imagined that they understood the safety and effectiveness of ED drugs and that their quality of life was improved by their partner's use of the drugs. Lack of information about ED drugs was a significant predictor for acceptance of a partner's use of ED drugs among women with a negative image of ED drugs, since they were more likely to accept the use of these drugs if they were convinced about their safety and effectiveness or positive effect on quality of life. Lack of information about ED drugs may influence the perception of women in Japan regarding these drugs.

  17. KiSS-1-mediated suppression of the invasive ability of human pancreatic carcinoma cells is not dependent on the level of KiSS-1 receptor GPR54

    Science.gov (United States)

    WANG, CHUN-HUI; QIAO, CHONG; WANG, RUO-CHEN; ZHOU, WEN-PING

    2016-01-01

    The onset of local invasion and lymphatic metastasis in pancreatic cancer limits survival following surgical intervention and additional therapies. Reduced expression of KiSS-1 in pancreatic cancer is associated with cancer metastasis. Previous studies have indicated that kisspeptin, the KiSS-1 peptide, is able to bind to its receptor-GPR54 (hOT7T175) and suppress the migration of PANC-1 pancreatic cancer cells. Whether the metastatic suppression of KiSS-1 is dependent on the levels of GPR54 in pancreatic cancer cell lines remains unclear. Human BxPC-3 pancreatic carcinoma cells are highly differentiated without exhibiting metastasis, however PANC-1 pancreatic carcinoma cells are poorly differentiated and exhibit local and lymph node metastasis. Compared with primary cultured trophoblasts, BxPc-3 and PANC-1 cells were observed to express low levels of KiSS-1 mRNA and protein, measured using reverse transcription-quantitative polymerase chain reaction and western blotting, respectively. However, greater mRNA and protein expression levels of GPR54 were observed in PANC-1 cells compared with BxPc-3 cells. An MTT assay was used to investigate the effect of KiSS-1 on BxPc-3 and PANC-1 cell proliferation. There were no significant differences in proliferation following transfection with KiSS-1 in BxPc-3 and PANC-1 cells compared with the controls (P>0.05). A Transwell assay with chambers coated with Matrigel was used to evaluate the in vitro invasive ability of BxPc-3 and PANC-1 cells, with the invasion index of BxPc-3 and PANC-1 cells significantly reduced following 48 h of KiSS-1 overexpression (PGPR54 expression was not altered (P>0.05). KiSS-1 is a metastasis suppressor gene of pancreatic cancer, and this suppression is not dependent on the expression levels of GPR54. Therefore, KiSS-1 is potentially a novel target for gene therapy. PMID:26572251

  18. A second wave of Salmonella T3SS1 activity prolongs the lifespan of infected epithelial cells

    Science.gov (United States)

    2017-01-01

    Type III secretion system 1 (T3SS1) is used by the enteropathogen Salmonella enterica serovar Typhimurium to establish infection in the gut. Effector proteins translocated by this system across the plasma membrane facilitate invasion of intestinal epithelial cells. One such effector, the inositol phosphatase SopB, contributes to invasion and mediates activation of the pro-survival kinase Akt. Following internalization, some bacteria escape from the Salmonella-containing vacuole into the cytosol and there is evidence suggesting that T3SS1 is expressed in this subpopulation. Here, we investigated the post-invasion role of T3SS1, using SopB as a model effector. In cultured epithelial cells, SopB-dependent Akt phosphorylation was observed at two distinct stages of infection: during and immediately after invasion, and later during peak cytosolic replication. Single cell analysis revealed that cytosolic Salmonella deliver SopB via T3SS1. Although intracellular replication was unaffected in a SopB deletion mutant, cells infected with ΔsopB demonstrated a lack of Akt phosphorylation, earlier time to death, and increased lysis. When SopB expression was induced specifically in cytosolic Salmonella, these effects were restored to levels observed in WT infected cells, indicating that the second wave of SopB protects this infected population against cell death via Akt activation. Thus, T3SS1 has two, temporally distinct roles during epithelial cell colonization. Additionally, we found that delivery of SopB by cytosolic bacteria was translocon-independent, in contrast to canonical effector translocation across eukaryotic membranes, which requires formation of a translocon pore. This mechanism was also observed for another T3SS1 effector, SipA. These findings reveal the functional and mechanistic adaptability of a T3SS that can be harnessed in different microenvironments. PMID:28426838

  19. Metabolic and Transcriptomic Changes Induced in Arabidopsis by the Rhizobacterium Pseudomonas fluorescens SS1011[W][OA

    Science.gov (United States)

    van de Mortel, Judith E.; de Vos, Ric C.H.; Dekkers, Ester; Pineda, Ana; Guillod, Leandre; Bouwmeester, Klaas; van Loon, Joop J.A.; Dicke, Marcel; Raaijmakers, Jos M.

    2012-01-01

    Systemic resistance induced in plants by nonpathogenic rhizobacteria is typically effective against multiple pathogens. Here, we show that root-colonizing Pseudomonas fluorescens strain SS101 (Pf.SS101) enhanced resistance in Arabidopsis (Arabidopsis thaliana) against several bacterial pathogens, including Pseudomonas syringae pv tomato (Pst) and the insect pest Spodoptera exigua. Transcriptomic analysis and bioassays with specific Arabidopsis mutants revealed that, unlike many other rhizobacteria, the Pf.SS101-induced resistance response to Pst is dependent on salicylic acid signaling and not on jasmonic acid and ethylene signaling. Genome-wide transcriptomic and untargeted metabolomic analyses showed that in roots and leaves of Arabidopsis plants treated with Pf.SS101, approximately 1,910 genes and 50 metabolites were differentially regulated relative to untreated plants. Integration of both sets of “omics” data pointed to a prominent role of camalexin and glucosinolates in the Pf.SS101-induced resistance response. Subsequent bioassays with seven Arabidopsis mutants (myb51, cyp79B2cyp79B3, cyp81F2, pen2, cyp71A12, cyp71A13, and myb28myb29) disrupted in the biosynthesis pathways for these plant secondary metabolites showed that camalexin and glucosinolates are indeed required for the induction of Pst resistance by Pf.SS101. Also for the insect S. exigua, the indolic glucosinolates appeared to play a role in the Pf.SS101-induced resistance response. This study provides, to our knowledge for the first time, insight into the substantial biochemical and temporal transcriptional changes in Arabidopsis associated with the salicylic acid-dependent resistance response induced by specific rhizobacteria. PMID:23073694

  20. RT-PCR Analysis of ED-A,ED-B, and IIICS Fibronectin Domains: A New Screening Marker For Bladder Cancer

    Directory of Open Access Journals (Sweden)

    M Ahmadi Javid

    2004-12-01

    Full Text Available Background: Fibronectin seems to play a very important role in the progression and invasion of bladder cancer. EDA, EDB, and IIICS domains of fibronectin are not expressed in the adult persons but they’re expressed in different cancers. The aim of this study is to investigate the mRNA of fibronectin in transitional carcinoma cells (TCC of bladder to study these domains. Methods: A total of 20 patients with known bladder cancer were studied. Two of them excluded since their excised tissues were not enough for both the pathological examination and RNA study. Another 20 (control group were normal volunteers who needed bladder operations. The excised tissue was immediately transferred to RNAlater (Ambion,TX. RNA was extracted via RNAWIZ (Ambion, TX. cDNA was made via RevertAid First Strand cDNA Synthesis Kit (Fermentas. PCR of the cDNAs was performed using primers for EDA, EDB, and IIICS (Eurogentec,Belgium. Results: For the first time, we present the expression of the oncofetal fibronectin mRNA in the transitional cell carcinoma of bladder. The high grade muscle invasive (G3T2 tumor, expressed ED-A, ED-B, and IIICS. Expression of ED-A, ED-B, and IIICS was confirmed in the two patients with G3T1 TCC. The four patients with G2Ta and G3Ta expressed both ED-A and ED-B. The four patients with G1T1 tumor expressed ED-A only, similar to the nine patients with G1Ta tumor. None of the normal volunteers expressed the oncofetal extra domains. The sensitivity of ED-A positive fibronectin RNA for detecting TCC of any kind is 100%, and of ED-B was only 35%. The specificity of ED-B positive fibronectin RNA for the high grade TCC is 100%. Conclusion: ED-A, ED-B, and IIICS could be used as useful markers for the diagnosis and following up of bladder carcinoma. Keywords: Transitional Cell Carcinoma, bladder cancer, fibronectin, RT-PCR, oncofetal.

  1. Patient-Centered Care Transition for Patients Admitted through the ED: Improving Patient and Employee Experience

    Directory of Open Access Journals (Sweden)

    Andrea Algauer BSN, RN

    2015-05-01

    Full Text Available With increasing wait times in emergency departments (ED across America, there is a need to streamline the inpatient admission process in order to decrease wait times and more important, to increase patient and employee satisfaction. One inpatient unit at New York-Presbyterian Weill Cornell Medical Center initiated a program to help expedite the inpatient admission process from the ED. The goal of the ED Bridge program is to ease the patient's transition from the ED to an inpatient unit by visiting the patient in the ED and introducing and setting expectations for the inpatient environment (i.e. telemetry alarms, roommates, hourly comfort rounds. Along with improving the patient experience, this program intends to improve the collaboration between ED nurses and inpatient nurses. With the continued support of our nurse management, hospital administrators and most important, our staff, this concept is aimed to increase patient satisfaction scores and subsequently employee satisfaction.

  2. Patient-Centered Care Transition for Patients Admitted through the ED: Improving Patient and Employee Experience.

    Science.gov (United States)

    Algauer, Andrea; Rivera, Stephanie; Faurote, Robert

    2015-05-01

    With increasing wait times in emergency departments (ED) across America, there is a need to streamline the inpatient admission process in order to decrease wait times and more important, to increase patient and employee satisfaction. One inpatient unit at New York-Presbyterian Weill Cornell Medical Center initiated a program to help expedite the inpatient admission process from the ED. The goal of the ED Bridge program is to ease the patient's transition from the ED to an inpatient unit by visiting the patient in the ED and introducing and setting expectations for the inpatient environment (i.e. telemetry alarms, roommates, hourly comfort rounds). Along with improving the patient experience, this program intends to improve the collaboration between ED nurses and inpatient nurses. With the continued support of our nurse management, hospital administrators and most important, our staff, this concept is aimed to increase patient satisfaction scores and subsequently employee satisfaction.

  3. SED-ED, a workflow editor for computational biology experiments written in SED-ML.

    Science.gov (United States)

    Adams, Richard R

    2012-04-15

    The simulation experiment description markup language (SED-ML) is a new community data standard to encode computational biology experiments in a computer-readable XML format. Its widespread adoption will require the development of software support to work with SED-ML files. Here, we describe a software tool, SED-ED, to view, edit, validate and annotate SED-ML documents while shielding end-users from the underlying XML representation. SED-ED supports modellers who wish to create, understand and further develop a simulation description provided in SED-ML format. SED-ED is available as a standalone Java application, as an Eclipse plug-in and as an SBSI (www.sbsi.ed.ac.uk) plug-in, all under an MIT open-source license. Source code is at https://sed-ed-sedmleditor.googlecode.com/svn. The application itself is available from https://sourceforge.net/projects/jlibsedml/files/SED-ED/.

  4. Intra-instar larval cannibalism in Anopheles gambiae (s.s. and Anopheles stephensi (Diptera: Culicidae

    Directory of Open Access Journals (Sweden)

    Daniele Porretta

    2016-11-01

    Full Text Available Abstract Background Cannibalism has been observed in a wide range of animal taxa and its importance in persistence and stability of populations has been documented. In anopheline malaria vectors the inter-instar cannibalism between fourth- and first-instar larvae (L4-L1 has been shown in several species, while intra-instar cannibalism remains poorly investigated. In this study we tested the occurrence of intra-instar cannibalism within larvae of second-, third- and fourth-instar (L2, L3 and L4 of Anopheles gambiae (s.s. and An. stephensi. Experiments were set up under laboratory conditions and the effects of larval density, duration of the contact period among larvae and the presence of an older larva (i.e. a potential cannibal of bigger size on cannibalism rate were analysed. Cannibalism was assessed by computing the number of missing larvae after 24 and 48 h from the beginning of the experiments and further documented by records with a GoPro videocamera. Results Intra-instar cannibalism was observed in all larval instars of both species with higher frequency in An. gambiae (s.s. than in An. stephensi. In both species the total number of cannibalistic events increased from 0–24 to 0–48 h. The density affected the cannibalism rate, but its effect was related to the larval instar and to the presence of older larvae. Interestingly, the lower cannibalism rate between L4 larvae was observed at the highest density and the cannibalism rate between L3 larvae decreased when one L4 was added. Conclusions The present study provides experimental evidence of intra-instar cannibalism in the malaria vectors An. gambiae (s.s. and An. stephensi and highlights the possible occurrence of complex interactions between all larval instars potentially present in the breeding sites. We hypothesize that the high density and the presence of a potential cannibal of bigger size could affect the readiness to attack conspecifics, resulting into low risk larval behavior

  5. Intra-instar larval cannibalism in Anopheles gambiae (s.s.) and Anopheles stephensi (Diptera: Culicidae).

    Science.gov (United States)

    Porretta, Daniele; Mastrantonio, Valentina; Crasta, Graziano; Bellini, Romeo; Comandatore, Francesco; Rossi, Paolo; Favia, Guido; Bandi, Claudio; Urbanelli, Sandra

    2016-11-02

    Cannibalism has been observed in a wide range of animal taxa and its importance in persistence and stability of populations has been documented. In anopheline malaria vectors the inter-instar cannibalism between fourth- and first-instar larvae (L4-L1) has been shown in several species, while intra-instar cannibalism remains poorly investigated. In this study we tested the occurrence of intra-instar cannibalism within larvae of second-, third- and fourth-instar (L2, L3 and L4) of Anopheles gambiae (s.s.) and An. stephensi. Experiments were set up under laboratory conditions and the effects of larval density, duration of the contact period among larvae and the presence of an older larva (i.e. a potential cannibal of bigger size) on cannibalism rate were analysed. Cannibalism was assessed by computing the number of missing larvae after 24 and 48 h from the beginning of the experiments and further documented by records with a GoPro videocamera. Intra-instar cannibalism was observed in all larval instars of both species with higher frequency in An. gambiae (s.s.) than in An. stephensi. In both species the total number of cannibalistic events increased from 0-24 to 0-48 h. The density affected the cannibalism rate, but its effect was related to the larval instar and to the presence of older larvae. Interestingly, the lower cannibalism rate between L4 larvae was observed at the highest density and the cannibalism rate between L3 larvae decreased when one L4 was added. The present study provides experimental evidence of intra-instar cannibalism in the malaria vectors An. gambiae (s.s.) and An. stephensi and highlights the possible occurrence of complex interactions between all larval instars potentially present in the breeding sites. We hypothesize that the high density and the presence of a potential cannibal of bigger size could affect the readiness to attack conspecifics, resulting into low risk larval behavior and lower cannibalism rate. The understanding of

  6. Reprint of "Echinococcus granulosus sensu stricto (s.s.) from the critically endangered antelope Addax nasomaculatus in Tunisia".

    Science.gov (United States)

    Boufana, Belgees; Saïd, Yousra; Dhibi, Mokhtar; Craig, Philip S; Lahmar, Samia

    2017-01-01

    Echinococcus granulosus sensu lato (s.l.) is a zoonotic disease highly endemic in Tunisia. Canids including stray and semi-stray dogs, jackals and foxes are known as definitive hosts and a wide range of ungulates have been shown to harbour the metacestode hydatid stage and may serve as intermediate hosts. Fertile hydatid cysts of Echinococcus equinus and E. granulosus sensu stricto (s.s.) were recently molecularly identified for the first time from Tunisian donkeys. E. granulosus (s.s.) was also identified from wild boars in Tunisia. Here we report the confirmation of hydatid cysts caused by E. granulosus (s.s.) in the critically endangered antelope, Addax nasomaculatus in Tunisia. DNA-based molecular analysis revealed that A. nasomaculatus was infected with E. granulosus (s.s.) which had a 100% identity with the main globally distributed E. granulosus (s.s.) (EgTu01) haplotype. Cysts of Taenia hydatigena (n=33) were also observed on the liver and in the body cavity. Due to their endangered status and their relatively small numbers, it is unlikely that hydatid infection of A. nasomaculatus will form a major contribution to the epidemiology and transmission of E. granulosus in Tunisia, but infection may result in pathology, morbidity and early mortality, and may still play a role in the perpetuation of the parasite in wildlife cycles. Copyright © 2015 Elsevier B.V. All rights reserved.

  7. Microstructural and Electrochemical Evaluation of Fusion Welded Low-Nickel and 304 SS at Different Heat Input

    Science.gov (United States)

    Bansod, Ankur V.; Patil, Awanikumar P.; Moon, Abhijeet P.; Shukla, Sourabh

    2017-11-01

    The present research study investigates the effect of heat input using E 308 electrode (controlled by welding current, i.e., 70, 85 and 100 A) on microstructure, mechanical properties and corrosion behavior of low-nickel and 304 stainless steel (SS) weldments produced by shielded metal arc welding technique. SEM investigation shows that with the higher heat input, δ-ferrite content was reduced. Dendrite and inter-dendritic length is also reduced by lowering the heat input. For all the heat inputs, it is observed that δ-ferrite content was higher in 304 stainless steel (SS) as compared to that of low-nickel austenitic stainless steel (Cr-Mn SS). Considering the heat input for Cr-Mn SS, coarse grains were observed in the heat-affected zone region. For low heat input (LHI), tensile fracture surface has exhibited river-like pattern with dimple appearance. Corrosion studies show better pitting resistance for low heat input (LHI) samples due to higher δ-ferrite present in the weld region. Similarly, higher interphase corrosion resistance is observed in both the SS grades causing more dissolution in the LHI samples.

  8. Roles of L5-7 loop in the structure and chaperone function of SsHSP14.1.

    Science.gov (United States)

    Wen, Zhen-Zhen; Wang, Yong-Hua; Yang, Bo; Xie, Ming-Quan; Chou, Kuo-Chen

    2011-03-01

    The small heat shock protein SsHSP14.1 from the hyper-thermophilic archeaon, Sulfolobus solfataricus (S. solfataricus) was able to protect proteins from thermal aggregation and prevent enzymes from heat induced inactivation. According to the 3D (dimensional) structural model of SsHSP14.1 developed by us before, the region L5-7 (β5-β7, 68-82 residues) plays an important role for the oligomerization of SsHSP14.1 and its chaperone function. Here, to validate the findings, an in-depth investigation was conducted of both the wild type SsHSP14.1 and its deletion mutant DEL75-79. With E. coli proteins and bromelain as substrate, the deletion mutant DEL75-79 can protect them from thermo-aggregating as effective as the wild protein. Interestingly, unlike the wild protein, DEL75-79 was unable to prevent bromelain and EcoRI from thermo-inactivating. Results of size exclusion HPLC showed that the oligomerization state was changed in mutant protein. This was in accordance with the changed structure and lower hydrophobicity of DEL75-79. These outcomes proved that the L5-7 loop did play a role for the oligomerizing SsHSP14.1, and that the residues 75-79 were indispensable for its function of prevent enzymes from thermo-inactivating.

  9. Microstructural and Electrochemical Evaluation of Fusion Welded Low-Nickel and 304 SS at Different Heat Input

    Science.gov (United States)

    Bansod, Ankur V.; Patil, Awanikumar P.; Moon, Abhijeet P.; Shukla, Sourabh

    2017-12-01

    The present research study investigates the effect of heat input using E 308 electrode (controlled by welding current, i.e., 70, 85 and 100 A) on microstructure, mechanical properties and corrosion behavior of low-nickel and 304 stainless steel (SS) weldments produced by shielded metal arc welding technique. SEM investigation shows that with the higher heat input, δ-ferrite content was reduced. Dendrite and inter-dendritic length is also reduced by lowering the heat input. For all the heat inputs, it is observed that δ-ferrite content was higher in 304 stainless steel (SS) as compared to that of low-nickel austenitic stainless steel (Cr-Mn SS). Considering the heat input for Cr-Mn SS, coarse grains were observed in the heat-affected zone region. For low heat input (LHI), tensile fracture surface has exhibited river-like pattern with dimple appearance. Corrosion studies show better pitting resistance for low heat input (LHI) samples due to higher δ-ferrite present in the weld region. Similarly, higher interphase corrosion resistance is observed in both the SS grades causing more dissolution in the LHI samples.

  10. Noninvasive cross-sectional imaging of proximal caries using swept-source optical coherence tomography (SS-OCT) in vivo.

    Science.gov (United States)

    Shimada, Yasushi; Nakagawa, Hisaichi; Sadr, Alireza; Wada, Ikumi; Nakajima, Masatoshi; Nikaido, Toru; Otsuki, Masayuki; Tagami, Junji; Sumi, Yasunori

    2014-07-01

    The aim of this study was to determine the diagnostic accuracy of swept-source optical coherent tomography (SS-OCT) in detecting and estimating the depth of proximal caries in posterior teeth in vivo. SS-OCT images and bitewing radiographs were obtained from 86 proximal surfaces of 53 patients. Six examiners scored the locations according to a caries lesion depth scale (0-4) using SS-OCT and the radiographs. The results were compared with clinical observations obtained after the treatment. SS-OCT could detect the presence of proximal caries in tomograms that were synthesized based on the backscatter signal obtained from the proximal carious lesion through occlusal enamel. SS-OCT showed significantly higher sensitivity and larger area under the receiver operating characteristic curve than radiographs for the detection of cavitated enamel lesions and dentin caries (Student's t -test, p proximal lesions in the clinical environment. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. X-linked anhidrotic ectodermal dysplasia (ED1 in men, mice, and cattle

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    Drögemüller Cord

    2003-06-01

    Full Text Available Abstract Ectodermal dysplasias are a large group of rare genetic disorders characterized by impaired development of hair, teeth, and eccrine glands in humans, mice, and cattle. Here, we review the cloning, mutation analyses, and functional studies of the known causative genes for the X-chromosomal anhidrotic ectodermal dysplasia (ED1 in these species. Mutations in the ectodysplasin 1 (ED1 gene are responsible for X-linked anhidrotic ectodermal dysplasia. The ED1 gene encodes a signaling molecule of the tumor necrosis factor family that is involved in development of ectodermal appendages. The bovine disorder may serve as an animal model for human ED1.

  12. A Preliminary Examination of Loss of Control Eating Disorder (LOC-ED) in Middle Childhood

    Science.gov (United States)

    Matherne, Camden E.; Tanofsky-Kraff, Marian; Altschul, Anne M.; Shank, Lisa M.; Schvey, Natasha A.; Brady, Sheila M.; Galescu, Ovidiu; Demidowich, Andrew P.; Yanovski, Susan Z.; Yanovski, Jack A.

    2015-01-01

    Loss of Control Eating Disorder (LOC-ED) has been proposed as a diagnostic category for children 6–12y with binge-type eating. However, characteristics of youth with LOC-ED have not been examined. We tested the hypothesis that the proposed criteria for LOC-ED would identify children with greater adiposity, more disordered eating attitudes, and greater mood disturbance than those without LOC-ED. Participants were 251 youth (10.29y ± 1.54, 53.8% female, 57.8 % White, 35.5% Black, 2.0% Asian, 4.8% Hispanic, 53.0% overweight). Youth were interviewed regarding eating attitudes and behaviors, completed questionnaires to assess general psychopathology, and underwent measurements of body fat mass. Using previously proposed criteria for LOC-ED, children were classified as LOC-ED (n = 19), LOC in the absence of the full disorder (subLOC, n = 33), and youth not reporting LOC (noLOC, n = 199). LOC-ED youth had higher BMIz (p = 0.001) and adiposity (p = 0.003) and reported greater disordered eating concerns (p disordered eating attitudes (p = 0.02). SubLOC youth had greater disordered eating concerns (p disordered eating cognitions and anthropometric measures compared to youth without LOC-ED. Longitudinal studies are needed to determine if those with LOC-ED are at particularly increased risk for progression of disordered eating and excess weight gain. PMID:25913008

  13. 8 Different approaches needed to manage ED demand among different age-groups.

    Science.gov (United States)

    Rimmer, Melanie; Ablard, Suzanne; O'Keeffe, Colin; Mason, Suzanne

    2017-12-01

    A variety of interventions have been proposed to manage rising demand for Emergency and Urgent Care, described by an NHS England review as unsustainable in the long term. However it is unlikely that any suggested approach will be equally suitable for the diverse population of ED users.We aimed to understand the patterns of demand amongst different types of patients attending ED. We also sought to understand the intended and unintended effects of demand management initiatives. Our study combined insights from routine data, a survey of ED patients, and qualitative interviews with ED staff. This paper describes the results of our analysis of the interviews. We conducted semi-structured interviews with 25 ED and Urgent Care Centre staff across 7 hospital sites in Yorkshire and Humber between 25 April and 11 July 2016. The interview topic guide asked about 4 broad areas; job role, description of patients and their impact on demand, description of inappropriate attendance, and current/future initiatives to deal with rising demand. Interviews were transcribed verbatim and analysed using framework analysis. We analysed the results to identify groups of patients with different patterns of use of ED services. We also explored ED staff experiences of demand management initiatives, and their suggestions for future initiatives. Although we did not ask specifically about patients' age, our analysis revealed that ED staff categorised attenders as children and young people, working age people, and older people. These groups had different reasons for attendance, different routes to the ED, different rate of non-urgent attendance, and different issues driving demand. Staff also described variation in the time taken to treat patients of different ages, with the oldest and youngest patients described as requiring the most time.There was no consensus amongst staff about the effectiveness of initiatives for managing demand. A strikingly wide variety of initiatives were mentioned

  14. DOE/PSU Graduate Student Fellowship Program for Hydropower

    Energy Technology Data Exchange (ETDEWEB)

    Cimbala, John M. [Pennsylvania State Univ., State College, PA (United States)

    2014-03-30

    The primary objective of this project is to stimulate academic interest in the conventional hydropower field by supplying research support for at least eight individual Master of Science (MS) or Doctoral (PhD) level research projects, each consisting of a graduate student supervised by a faculty member. We have completed many of the individual student research projects: 2 PhD students have finished, and 4 are still working towards their PhD degree. 4 MS students have finished, and 2 are still working towards their MS degree, one of which is due to finish this April. In addition, 4 undergraduate student projects have been completed, and one is to be completed this April. These projects were supervised by 7 faculty members and an Advisory/Review Panel. Our students and faculty have presented their work at national or international conferences and have submitted several journal publications. Three of our graduate students (Keith Martin, Dan Leonard and Hosein Foroutan) have received HRF Fellowships during the course of this project. All of the remaining students are anticipated to be graduated by the end of Fall Semester 2014. All of the tasks for this project will have been completed once all the students have been graduated, although it will be another year or two until all the journal publications have been finalized based on the work performed as part of this DOE Hydropower project.

  15. PREFACE: The 4th Symposium on the Mechanics of Slender Structures (MoSS2013)

    Science.gov (United States)

    Cao, Dengqing; Kaczmarczyk, Stefan

    2013-07-01

    This volume of Journal of Physics: Conference Series contains papers presented at the 4th Symposium on the Mechanics of Slender Structures (MoSS2013) run under the auspices of the Institute of Physics Applied Mechanics Group and hosted by Harbin Institute of Technology (China) from 7-9 January 2013. The conference has been organized in collaboration with the Technical Committee on Vibration and Sound of the American Society of Mechanical Engineers and follows a one day seminar on Ropes, Cables, Belts and Chains: Theory and Applications and the MoSS2006 symposium held at the University of Northampton (UK) in 2004 and 2006, respectively, the MoSS2008 symposium held at the University of Maryland Baltimore County (USA) in 2008 and the MoSS2010 symposium hosted by Mondragon University and held in San Sebastian (Spain) in 2010. The remit of the Symposium on the Mechanics of Slender Structures series involves a broad range of scientific areas. Applications of slender structures include terrestrial, marine and space systems. Moving elastic elements such as ropes, cables, belts and tethers are pivotal components of many engineering systems. Their lengths often vary when the system is in operation. The applications include vertical transportation installations and, more recently, space tether propulsion systems. Traction drive elevator installations employ ropes and belts of variable length as a means of suspension, and also for the compensation of tensile forces over the traction sheave. In cranes and mine hoists, cables and ropes are subject to length variation in order to carry payloads. Tethers experiencing extension and retraction are important components of offshore and marine installations, as well as being proposed for a variety of different space vehicle propulsion systems based on different applications of momentum exchange and electrodynamic interactions with planetary magnetic fields. Furthermore, cables and slender rods are used extensively in civil engineering

  16. Hoitajien stressitekijät ja niiden vähennyskeinot saattohoitotyössä

    OpenAIRE

    Mäenluoma, Aino; Mäkinen, Marika

    2016-01-01

    Tämän opinnäytetyön tarkoituksena oli kuvata hoitajien stressitekijöitä ja tuoda esille niitä vähentäviä keinoja. Tavoitteena oli tuottaa tietoa hoitajien stressitekijöistä saattohoitotyössä ja koota yhteen stressiä vähentäviä keinoja, jotta hoitajat pystyisivät tulevaisuudessa käyttämään niitä, jotta hoitajat pystyisivät hyödyntämään niitä ja työhyvinvointia voitaisiin parantaa. Opinnäytetyö toteutettiin ryhmähaastattelulla, joka teemoitettiin. Aineisto kerättiin syyskuussa 2016. ...

  17. RICOMPOSIZIONE VIRTUALE DEL TABERNACOLO DI ISAIA DA PISA PER LA CHIESA DELLA SS. TRINITÀ DI VITERBO

    Directory of Open Access Journals (Sweden)

    Dante Abate

    2012-04-01

    Full Text Available

    Questo progetto ha preso spunto da uno studio condotto da Giusy Zevolini e pubblicato nel 2003 sulla Rivista dell’Istituto Nazionale di Archeologia e Storia dell’Arte. Nell’articolo la studiosa proponeva un’ipotesi di ricomposizione di due opere scultoree in marmo, entrambe eseguite durante il XV secolo, una delle quali sicuramente dall’artista toscano Isaia da Pisa. Le due parti, in origine probabilmente inserite in un unico complesso scultoreo monumentale, all’interno della chiesa agostiniana della SS. Trinità di Viterbo, oggi sono conservate in due siti distinti del capoluogo del territorio della Tuscia.

    Virtual Reconstruction of the Tabernacolo of Isaia da Pisa for the Church of SS. Trinità in Viterbo

    This study is based upon a characteristic, Art History paper, published in 2003. Specifically it suggests a reconstruction hypotesis concerning two marble objects, created in the XV Century, which were supposed to belong to each other, in a monumental complex, inside the Augustinian Church of the SS. Trinità in Viterbo (Italy. In the article the iconography was rendered by simply stitching pictures together, losing the objects proportions, without any care given to the likely missing parts, and of course showing the image in 2D. Despite scholars mostly agreeing that the two marble artefacts were made by the same artist (Isaiada Pisa and that both belong to the same church, they are still notsure if the pieces are a unique structure. The virtual re-composition helps to enhance the visual perception of the whole construction, which is now available just in 2D flat images, and to understand how it originally would have looked. The two objects, today placed in different locations in the city of Viterbo, studied in this paper are: a marble tabernacle with Angels and Christ; a marble tympanum with a blessing by Christ. Both sculptures were acquired witha triangulation laser scanner in two different

  18. Tiimityön kehittäminen ministeriössä

    OpenAIRE

    Kuosmanen, Jaana

    2012-01-01

    Tämän opinnäytetyön toimeksiantajana on Valtiovarainministeriön Hallinnon kehittä-misosasto (nykyisin uudelleenorganisoitunut), joka on kehittänyt tiimityöskentelyään. Opinnäytetyön tarkoituksena oli selvittää, minkälaisia tiimityön teorioita ja oppeja Hallinnon kehittämisosasto on ottanut esille kehittämistyössään, ja tutkia niiden soveltuvuutta käytännön hanketyöhön, jota on tarkoitus toteuttaa tiimimäisesti. Tämän työn teoreettisessa viitekehyksessä käsitellään sellaisia tiimeihin ja t...

  19. Reduction of salt-requirement of halophilic nucleoside diphosphate kinase by engineering S-S bond.

    Science.gov (United States)

    Ishibashi, Matsujiro; Uchino, Manami; Arai, Shigeki; Kuroki, Ryota; Arakawa, Tsutomu; Tokunaga, Masao

    2012-09-01

    Nucleoside diphosphate kinase (HsNDK) from extremely halophilic haloarchaeon, Halobacterium salinarum, requires salt at high concentrations for folding. A D148C mutant, in which Asp148 was replaced with Cys, was designed to enhance stability and folding in low salt solution by S-S bond. It showed increased thermal stability by about 10 °C in 0.2 M NaCl over the wild type HsNDK. It refolded from heat-denaturation even in 0.1 M NaCl, while the wild type required 2 M NaCl to achieve the same level of activity recovery. This enhanced refolding is due to the three S-S bonds between two basic dimeric units in the hexameric HsNDK structure, indicating that assembly of the dimeric unit may be the rate-limiting step in low salt solution. Circular dichroism and native-PAGE analysis showed that heat-denatured HsNDK formed partially folded dimeric structure, upon refolding, in the absence of salt and the native-like secondary structure in the presence of salt above 0.1 M NaCl. However, it remained dimeric upon prolonged incubation at this salt concentration. In contrary, heat-denatured D148C mutant refolded into tetrameric folding intermediate in the absence of salt and native-like structure above 0.1 M salt. This native-like structure was then converted to the native hexamer with time. Copyright © 2012 Elsevier Inc. All rights reserved.

  20. The effect of topography of upper-mantle discontinuities on SS precursors

    Science.gov (United States)

    Koroni, Maria; Trampert, Jeannot

    2016-01-01

    Using the spectral-element method, we explored the effect of topography of upper-mantle discontinuities on the traveltimes of SS precursors recorded on transverse component seismograms. The latter are routinely used to infer the topography of mantle transition zone discontinuities. The step from precursory traveltimes to topographic changes is mainly done using linearised ray theory, or sometimes using finite-frequency kernels. We simulated exact seismograms in 1-D and 3-D elastic models of the mantle. In a second simulation, we added topography to the discontinuities. We compared the waveforms obtained with and without topography by cross correlation of the SS precursors. Since we did not add noise, the precursors are visible in individual seismograms without the need of stacking. The resulting time anomalies were then converted into topographic variations and compared to the original topographic models. Based on the correlation between initial and inferred models, and provided that ray coverage is good, we found that linearised ray theory gives a relatively good idea on the location of the uplifts and depressions of the discontinuities. It seriously underestimates the amplitude of the topographic variations by a factor ranging between 2 and 7. Real data depend on the 3-D elastic structure and the topography. All studies to date correct for the 3-D elastic effects assuming that the traveltimes can be linearly decomposed into a structure and a discontinuity part. We found a strong non-linearity in this decomposition which cannot be modelled without a fully non-linear inversion for elastic structure and discontinuities simultaneously.

  1. Discriminative feeding behaviour of Anopheles gambiae s.s. on endemic plants in western Kenya.

    Science.gov (United States)

    Manda, H; Gouagna, L C; Nyandat, E; Kabiru, E W; Jackson, R R; Foster, W A; Githure, J I; Beier, J C; Hassanali, A

    2007-03-01

    Anopheles gambiae Giles s.s. (Diptera: Culicidae) is known to feed on plant sugars, but this is the first experimental study to consider whether it discriminates between plant species. Thirteen perennial plant species were selected on the basis of their local availability within the vicinity of human dwellings and larval habitats of An. gambiae s.s. in western Kenya. Groups of 100 or 200 mosquitoes were released into cages either with a cutting of one plant type at a time (single-plant assay) or with cuttings of all 13 plants simultaneously (choice assay), respectively, and left overnight. In the choice assay, direct observations of the percentages of mosquitoes perching or feeding on each plant were recorded over four 1-h periods each night. For both types of assay, mosquitoes were recaptured and the percentage that had fed on plants was assessed by testing them individually for the presence of fructose. To identify which plants the choice-assay mosquitoes had fed on, gas chromatography (GC) profiles of samples of mosquito homogenates were compared with GC profiles of extracts from relevant parts of each plant. Four of the plants that were observed to have been fed on most frequently in the choice assay (Parthenium hysterophorus L., Tecoma stans L., Ricinus communis L., and Senna didymobotrya Fresen) were also shown to have been ingested most often by mosquitoes in both types of assay, suggesting that An. gambiae is differentially responsive to this range of plants, regardless of whether the plants were presented singly or mixed together. Significantly more females than males fed on plants, with the exception of P. hysterophorus L., one of the plants most frequently fed on. For most plant species (ten of 13), GC profiles indicated that An. gambiae obtained sugars primarily from flowers. The exceptions were P. hysterophorus L., Lantana camara L. and R. communis L., on which An. gambiae fed more often from leaves and stems than from flowers.

  2. Interacting large-scale magnetic fields and ionized gas in the W50/SS433 system

    Science.gov (United States)

    Farnes, J. S.; Gaensler, B. M.; Purcell, C.; Sun, X. H.; Haverkorn, M.; Lenc, E.; O'Sullivan, S. P.; Akahori, T.

    2017-06-01

    The W50/SS433 system is an unusual Galactic outflow-driven object of debatable origin. We have used the Australia Telescope Compact Array to observe a new 198 pointing mosaic, covering 3° × 2°, and present the highest-sensitivity full-Stokes data of W50 to date using wide-field, wide-band imaging over a 2 GHz bandwidth centred at 2.1 GHz. We also present a complementary Hα mosaic created using the Isaac Newton Telescope Photometric Hα Survey of the Northern Galactic Plane. The magnetic structure of W50 is consistent with the prevailing hypothesis that the nebula is a reanimated shell-like supernova remnant (SNR), which has been re-energized by the jets from SS433. We observe strong depolarization effects that correlate with diffuse Hα emission, likely due to spatially varying Faraday rotation measure (RM) fluctuations of ≥48-61 rad m-2 on scales ≤4.5-6 pc. We also report the discovery of numerous, faint, Hα filaments that are unambiguously associated with the central region of W50. These thin filaments are suggestive of an SNR's shock emission, and almost all have a radio counterpart. Furthermore, an RM-gradient is detected across the central region of W50, which we interpret as a loop magnetic field with a symmetry axis offset by ≈90° to the east-west jet-alignment axis, and implying that the evolutionary processes of both the jets and the SNR must be coupled. A separate RM-gradient is associated with the termination shock in the eastern ear, which we interpret as a ring-like field located where the shock of the jet interacts with the circumstellar medium. Future optical observations will be able to use the new Hα filaments to probe the kinematics of the shell of W50, potentially allowing for a definitive experiment on W50's formation history.

  3. Kinetics investigation of the hydrogen abstraction reaction between CH3SS and CN radicals.

    Science.gov (United States)

    Yan, Liu; Wenliang, Wang; Zhongwen, Liu; Hongjiang, Ren

    2016-01-01

    The reaction mechanisms and rates for the H abstraction reactions between CH3SS and CN radicals in the gas phase were investigated with density functional theory (DFT) methods. The geometries, harmonic vibrational frequencies, and energies of all stationary points were obtained at B3PW91/6-311G(d,p) level of theory. Relationships between the reactants, intermediates, transition states and products were confirmed, with the frequency and the intrinsic reaction coordinate (IRC) analysis at the same theoretical level. High accurate energy information was provided by the G3(MP2) method combined with the standard statistical thermodynamics. Gibbs free energies at 298.15 K for all of the reaction steps were reported, and were used to describe the profile diagrams of the potential energy surface. The rate constants were evaluated with both the classical transition state theory and the canonical variational transition state theory, in which the small-curvature tunneling correction was included. A total number of 9 intermediates (IMs) and 17 transition states (TSs) were obtained. It is shown that IM1 is the most stable intermediate by the largest energy release, and the channel of CH3SS + CN → IM3 → TS10  → P1(CH2SS + HCN) is the dominant reaction with the lowest energy barrier of 144.7 kJ mol(-1). The fitted Arrhenius expressions of the calculated CVT/SCT rate constants for the rate-determining step of the favorable channel is k =7.73 × 10(6)  T (1.40)exp(-14,423.8/T) s(-1) in the temperature range of 200-2000 K. The apparent activation energy E a(app.) for the main channel is -102.5 kJ mol(-1), which is comparable with the G3(MP2) energy barrier of -91.8 kJ mol(-1) of TS10 (relative to the reactants).

  4. Genome wide survey of microsatellites in ssDNA viruses infecting vertebrates.

    Science.gov (United States)

    Jain, Ankit; Mittal, Nikhil; Sharma, Prakash C

    2014-12-01

    Microsatellites or Simple Sequence Repeats (SSRs) are tandem iterations of one to six base pairs, non-randomly distributed throughout prokaryotic and eukaryotic genomes. Limited knowledge is available about distribution of microsatellites in single stranded DNA (ssDNA) viruses, particularly vertebrate infecting viruses. We studied microsatellite distribution in 118 ssDNA virus genomes belonging to three families of vertebrate infecting viruses namely Circoviridae, Parvoviridae, and Anelloviridae, and found that microsatellites constitute an important component of these virus genomes. Mononucleotide repeats were predominant followed by dinucleotide and trinucleotide repeats. A strong positive relationship existed between number of mononucleotide repeats and genome size among all the three virus families. A similar relationship existed for the occurrence of DTTPH (di-, tri-, tetra-, penta- and hexa-nucleotide) repeats in the families Anelloviridae and Parvoviridae only. Relative abundance and relative density of mononucleotide repeats showed a strong positive relationship with genome size in Circoviridae and Parvoviridae. However, in the case of DTTPH repeats, these features showed a strong relationship with genome size in Circoviridae only. On the other hand, relative microsatellite abundance and relative density of mononucleotide repeats were negatively correlated with GC content (%) in Parvoviridae genomes. On the basis of available annotations, our analysis revealed maximum occurrence of mononucleotide as well as DTTPH repeats in the coding regions of these virus genomes. Interestingly, after normalizing the length of the coding and non-coding regions of each virus genome, we found relative density of microsatellites much higher in the non-coding regions. We understand that the present study will help in the better characterization of the stability, genome organization and evolution of these virus classes and may provide useful leads to decipher the

  5. The MAL-ED cohort study in Mirpur, Bangladesh.

    Science.gov (United States)

    Ahmed, Tahmeed; Mahfuz, Mustafa; Islam, Md Munirul; Mondal, Dinesh; Hossain, Md Iqbal; Ahmed, Am Shamsir; Tofail, Fahmida; Gaffar, Sm Abdul; Haque, Rashidul; Guerrant, Richard L; Petri, William A

    2014-11-01

    The Etiology, Risk Factors and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development (MAL-ED) study site in Bangladesh is located in the capital city of Dhaka in an urban slum that has one of the highest population densities in the world. The site is in the Bauniabadh area of Mirpur, Dhaka. A typical squatter settlement, the average family size of households in Mirpur Bauniabadh is 4.5, with 48% females. About 20% of households have a monthly income of only US$62. About 30% of mothers never attended school, and only 3% obtained secondary school education. The majority of the people are day laborers, garment workers, and transport workers. About 72% of caregivers always wash their hands after helping the child defecate and 6.6% never wash their hands. The diarrheal attack rate for Mirpur is 4.69 episodes per child per year. The study site is representative of a typical urban slum of Dhaka city in terms of demographics, socioeconomic status, and general health indicators. © The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  6. On E.D. Jones' MICROCOSMOLOGY

    Energy Technology Data Exchange (ETDEWEB)

    Noyes, H. Pierre

    2003-01-08

    By taking seriously the limits on observability which come from combining relativistic quantum mechanics with general relativity, Ed Jones has shown that the current measurements of the cosmological constant density {Omega}{sub {Lambda}} {approx} 0.7 imply that the temperature scale at which it becomes possible to discuss cosmological models is {approx} 5 Tev (5.8 x 10{sup 16} K). This is self-consistent with the assumption that the number of Planck masses which make some sort of ''phase transition'' to this state is N{sub Pk} {approx} 4 x 10{sup 61}. We review Jones' argument and the bit-string physics calculation which gives the baryon-photon ratio at nucleosynthesis as {approx} 2/256{sup 4}, the dark matter-baryon ratio as {approx} 12.7, and hence {Omega}{sub m} {approx} 0.3, in agreement with current observations. Accepting these values for the two energy densities {Omega}{sub {Lambda}} + {Omega}{sub m} {approx} 1 in accord with recent analyses of fluctuations in the CMB showing that space is flat to about 6%. We conclude that experiments with particle accelerators in the 5-10 Tev range must either show that current theory can adequately describe the currently observed structure of our universe or force us to revise our ideas about physics at a very fundamental level.

  7. Spazio, movimento, prospettiva ed empatia: un prototipo di videogame didattico

    Directory of Open Access Journals (Sweden)

    Pio Alfredo Di Tore

    2014-09-01

    Full Text Available Questo lavoro presenta la cornice teorica che sta alla base della progettazione di un videogame didattico pensato per valutare le abilità di perspective taking (capacità di adottare la prospettiva altrui e di mental rotation (rotazione mentale dei giocatori. Lo studio mira a rilevare come tali abilità siano coinvolte nella relazione empatica e a verificarne le implicazioni in campo educativo. Il lavoro adotta la definizione di empatia riconducibile ad Alain Berthoz ed alla teoria spaziale dell’empatia qui presentata con i relativi sistemi di riferimento spaziale. È inoltre fornita una rapida revisione della letteratura sulla rappresentazione dello spazio nel bambino e sulla rappresentazione dello spazio nel gioco (su base visiva. Infine, il lavoro descrive il prototipo di gioco – realizzato presso l’Università di Salerno – in cui il giocatore si trova alle prese con tre differenti compiti di cui due progettati per misurare le abilità di perspective taking mentre il terzo è calibrato sulle abilità di mental rotation.

  8. Home modification to prevent falls by older ED patients.

    Science.gov (United States)

    Gerson, Lowell W; Camargo, Carlos A; Wilber, Scott T

    2005-05-01

    This trial was conducted at 11 EDs to test the effectiveness of distributing fall prevention information to patients 65 years or older. Intervention patients were given 2 brochures and received a reminder call 2 weeks later. All patients were called at 1 month and asked if they made home safety modifications. Three hundred ninety-seven patients were enrolled (118 control, 279 intervention). Seventy-six percent had complete follow up interviews. Nine percent of control and 8% of intervention patients reported a home modification (95% confidence interval on difference, -8.1% to 5.5%). Patients who fell in the prior year had a 2.0 increased odds (95% confidence interval, 0.8-4.6) of making a home modification. The similar home modification rates in the 2 study groups suggest that even minimum discussion (eg, the informed consent procedure) may increase patients' fall prevention activities. The stronger association in patients who fell suggests that a targeted program may have added benefit.

  9. Spirometrie v prostředí LabVIEW

    OpenAIRE

    Pernicová, Lenka

    2010-01-01

    Tato bakalářská práce se zabývá funkčním vyšetřením plic – spirometrií. Skládá se ze dvou základních částí. V první části je důležité seznámit se s jednotlivými metodami funkčního vyšetření plic. Na základě důkladného prostudování parametrů popisujících funkci plic a zaměření se na spirometrickou křivkou, bylo možné přejít k druhé části této práce, tzn. k realizaci spirometru v grafickém prostředí LabVIEW. Vytvořený virtuální přístroj nám umožňuje zobrazení spirometrické křivky, následnou ana...

  10. Alprostadil plus Vacuum (VITARUM in severe erectile dysfunction (ED

    Directory of Open Access Journals (Sweden)

    Franco Mantovani

    2017-06-01

    Full Text Available Objectives: Severe erectile dysfunction (ED is not uncommon, as can be seen from the epidemiological literature, and there are several possible causes, which are not always known, or leastways evident. Having ascertained the ineffectiveness, intolerance to or rejection of pharmacological aids, the option of prosthetic surgery remains, but before this, it may be wise when feasible to use Alprostadil cream in association with Vacuum device. Material and methods: 12 patients, aged between 55 and 65 years, with severe erectile dysfunction without palpable cavernous fibrosis, were instructed to self-insert into the urethral meatus, 3 mg of Alprostadil cream, sufficient to make it easy to place the Vacuum device over the penis. Results: In the cases observed, the preliminary use of Alprostadil cream fast produced an erection with enough rigidity to place the Vacuum. A sufficient erection was maintained, obviously using an elastic ring at the base of the penis, to achieve penetration. The reproducibility of the use of Alprostadil cream with Vacuum device was then confirmed at home, to the satisfaction of the patients.

  11. Ready-JET-Go: Split Flow Accelerates ED Throughput.

    Science.gov (United States)

    Bish, Peter A; McCormick, Mary A; Otegbeye, Mojisola

    2016-03-01

    Struggling to keep up with The Centers for Medicare and Medicaid Services out-patient throughput metrics, an adult emergency department serving Burlington and Camden Counties, New Jersey, sought to redefine its care delivery model by adopting the patient segmentation initiatives of the split-flow process of patient care. A multidisciplinary team of ED clinicians collaboratively defined the patient segmentation criteria. A joint assessment team approach to patient care was instituted. A 3-pronged approach was adopted to prepare staff for the patient care changes in line with an existing framework specified by the Institute of Medicine. Simulation and queuing analyses were used to estimate the accompanying resource needs. Since implementing split flow, the emergency department has witnessed significant improvements in patient throughput and patient satisfaction, despite a sustained 10% increase in patient volumes after split-flow implementation. The median length of stay for discharged patients and the door-to-diagnostic evaluation time are now down to 112 minutes and 30 minutes, respectively, compared with pre-split-flow values of 192 minutes and 72 minutes, respectively. Working collaboratively with all stakeholders to define the right patient care delivery model, combined with an understanding of the right resource assignments to optimally support that care delivery model, an emergency department can institute cost-effective changes to realize and sustain significant patient throughput improvements. Copyright © 2016 Emergency Nurses Association. Published by Elsevier Inc. All rights reserved.

  12. Book Review: Digital Forensic Evidence Examination (2nd ed.

    Directory of Open Access Journals (Sweden)

    Gary Kessler

    2010-06-01

    Full Text Available Cohen, F. (2010. Digital Forensic Evidence Examination (2nd ed.. Livermore, CA: ASP Press. 452 pages, ISBN: 978-1-878109-45-3, US$79.Reviewed by Gary C. Kessler, Gary Kessler Associates & Edith Cowan University (gck@garykessler.netOn the day that I sat down to start to write this review, the following e-mailcame across on one of my lists:Person A and Person B write back and forth and create an email thread. Person A then forwards the email to Person C, but changes some wording in the email exchange between A & B. What is the easiest way (and is it even possible to find out when that earlier email message was altered before sent to Person C?Before you try to answer these questions, read Fred Cohen's Digital Forensic Evidence Examination. His book won't actually tell you how to answer these questions but it will help you understand the difficulty in even trying to answer them with any level of certainty.(see PDF for full review

  13. Verbo come elemento della frase in friulano ed in frances

    Directory of Open Access Journals (Sweden)

    David Bizjak

    2003-12-01

    Full Text Available Il presente lavoro e dedicato a una parte della sfera del verbo nel friulano  lettera­ rio della seconda meta del ventesimo secolo, alla perifrasi verbale e alla locuzione verbale. Nella definizione della perifrasi verbale (PV ho seguito il modello dei lin­ guisti spagnoli Javier Garcia Gonzales, Fernandez de Castro e Leonard Gomez Torrego, essendo ilpunto di partenza ilmio postulato che la PV rappresenti in friu­ lano una categoria grammaticale a parte o, almeno, una categoria in via di gramma­ ticalizzazione. Parallelamente alla situazione in friulano osservo quella nel francese scritto contemporaneo, con lo scopo di constatare delle eventuali somiglianze e diffe­ renze. Tenendo in considerazione la realta linguistica nella regione Friuli-Venezia Giulia, dove le interferenze fra l'italiano, il friulano ed il veneto sono tali che un non-friulanofono non riesce facilmente a distinguere quando si tratta di un sintag­ ma di origine friulana e quando di un calco sintattico sull'italiano, sembra oppor­ tuno, in numerosi casi, confrontare ilsintagma friulano e quello francese anche con la variante corrispondente in italiano letterario moderno; inoltre, nel capitolo in cui sono trattati i cosiddetti tempi bicomposti, vengono citati alcuni esempi nelle diver­ se varieta venete.

  14. Etica ed evoluzionismo: la proposta di Marc Hauser

    Directory of Open Access Journals (Sweden)

    Irene Pilloni

    2013-11-01

    Full Text Available Le recenti scoperte nell’ambito della psicologia evoluzionistica potrebbero offrire una risposta al dibattito sull’origine evoluzionistica della facoltà morale dell’Homo sapiens. In passato, il tentativo di spiegare il comportamento morale a partire dalla teoria dell’evoluzione è stato intrapreso dal padre fondatore dell’evoluzionismo Charles Darwin, successivamente da T.H. Huxley e da H. Spencer e infine dal sociobiologo E. Wilson a metà degli anni ‘70 del Novecento. Oggi questa impresa è stata ereditata dallo psicologo evoluzionista Marc Hauser, le cui indagini prendono avvio da un’analogia tra facoltà morale e facoltà linguistica. Questa analogia gli consente di affermare che la pluralità di codici morali adottati dagli uomini nelle differenti culture dipende da un numero limitato di principi morali, nello stesso modo in cui la varietà di lingue con cui gli uomini si esprimono, dipende da un numero limitato di principi linguistici universali. Dunque, sembrerebbe che l’evoluzione biologica abbia plasmato dei principi morali universali e uniformi che si presentano costanti in tutti gli uomini a prescindere dalla loro appartenenza culturale. Come vadano intesi i principi morali universali e quale rapporto intercorre tra di essi e i vari codici morali sarà l’argomento di questo saggio, il quale tenterà di analizzare la proposta di Marc Hauser all’interno della cornice dei rapporti tra etica ed evoluzionismo.

  15. Sappiamo davvero come far apprendere? Credenza ed evidenza empirica.

    Directory of Open Access Journals (Sweden)

    Roberto Trinchero

    2013-08-01

    Full Text Available “I ragazzi con più stimoli apprendono di più e meglio di quelli sottoposti a meno stimoli!”, “In questa scuola non possiamo fare didattica attiva perché non abbiamo i laboratori!”, “I ragazzi imparano meglio se li si lascia sperimentare e costruire i concetti da soli!”, “La lezione frontale? Ormai è una strategia didattica superata!”, “Se hai capito il concetto una volta, poi lo saprai applicare sempre!”, “I ragazzi imparano meglio quando fanno lavori di gruppo!”. Quante volte avete sentito insegnanti ed educatori pronunciare queste frasi? Ma cosa dice la ricerca in proposito? Queste sono solo credenze o hanno davvero un fondamento empirico? Il presente articolo intende fornire alcune evidenze per supportare, confutare, chiarire le condizioni di validità degli asserti suddetti. Le evidenze sono tratte dalla ricerca sul funzionamento della mente e dalle meta-analisi che si sono occupate di sintetizzare i principali studi empirici riguardanti l’impatto di varie strategie didattiche sull’apprendimento dei soggetti in formazione.

  16. Työajan kohdentaminen uutena menettelynä : Menettelyn pilotointi liikenne- ja viestintäministeriössä

    OpenAIRE

    Ilpala, Hanna

    2016-01-01

    Opinnäytetyön tarkoituksena oli suunnitella ja toteuttaa työajan kohdentamisen pilotointi liikenne- ja viestintäministeriössä. Ministeriössä otetaan 1.4.2016 käyttöön valtion yhteinen talous- ja henkilöstöhallinnon tietojärjestelmä Kieku, jonka yhtenä osana on työajan kohdentaminen toiminnoille. Ministeriössä ei ole aikaisemmin kohdennettu työaikaa. Pilotoinnin tavoitteena oli harjoitella kohdentamista ja siten valmistaa henkilöstöä uuteen menettelyyn ja käyttöönottoon. Lisäksi tavoitteina ol...

  17. Towards a better understanding of intertribal relationships and stable tribal delimitations within Cinchonoideae s.s. (Rubiaceae).

    Science.gov (United States)

    Manns, Ulrika; Bremer, Birgitta

    2010-07-01

    This study focuses on the subfamily Cinchonoideae s.s. utilizing information from six DNA markers and 206 taxa. The nine tribes (i.e. Cinchoneae, Chiococceae s.l., Guettardeae s.s., Hamelieae, Hillieae, Hymenodictyeae, Isertieae, Naucleeae s.l., and Rondeletieae s.s.) are resolved in four major lineages, all strongly supported and relationships between them are resolved. The tropical American Cinchoneae and Isertieae constitute the first diverging lineage within the subfamily, followed by the predominantly paleotropical Naucleeae and Hymenodictyeae. The remaining two lineages primarily include neotropical taxa: Rondeletieae and Guettardeae are sister clades in the first, while the second comprises Chiococceae, Hamelieae, and Hillieae. Additionally, taxonomic placement of several genera, not previously included in molecular analyses, were confirmed: Acunaeanthus belongs in Rondeletieae, Ottoschmidtia in Guettardeae, Nernstia in Chiococceae, Pinarophyllon, Plocaniophyllon, and Syringantha in Hamelieae, and Balmea in Hillieae. Colleteria, of previously unknown taxonomic position, is resolved as sister to Chione. Copyright 2010 Elsevier Inc. All rights reserved.

  18. Decolorizing kinetics of a recombinant Escherichia coli SS125 strain harboring azoreductase gene from Bacillus latrosporus RRK1.

    Science.gov (United States)

    Sandhya, S; Sarayu, K; Uma, B; Swaminathan, K

    2008-05-01

    PCR amplified product containing gene responsible for dye decolorization was cloned and expressed in Escherichia coli. The resulting recombinant strain E. coli SS125 decolorized 200mg/l azo dye (Remazol Red) at 30 degrees C at 255 mg cell/l/h, while the host E. coli (DH5 alpha) had no color removal ability. The dependence of the decolorization rate on initial dye concentration and the maximum rate occurred with the dye at 100 mg l(-1). The decolorization rate of E. coli SS125 was optimal at 37-45 degrees C. Aeration strongly-inhibited the decolorization, but decolorization occurred effectively under static and anaerobic incubation conditions. The E. coli SS125 strain also exhibited excellent stability during reported batch operation.

  19. Hearing of Mr. Francois Roussely, President of EdF; Audition de M. Francois Roussely, President d'EdF

    Energy Technology Data Exchange (ETDEWEB)

    Roussely, F. [Electricite de France (EDF), 75 - Paris (France)

    2005-07-01

    This document is the proceedings of the hearing of F. Roussely, President of Electricite de France (EdF), at the commission of economic affairs of the French house of commons, about the advisability of the construction of the EPR (European pressurized reactor) demonstration plant and about its possible financing by EdF. In a first part, F. Roussely recalls the European context of deregulation of energy markets and its impact of the French electric power industry (opening of the French market, industrial and social actions of EdF, need of a new generation of nuclear reactor, preservation of EdF's energy mix, warranty of public utility, un-bundling between energy trade and distribution, EdF's turnover and profitability, EdF's foreign daughter companies). In a second part, F. Roussely answers a series of questions asked by the different members of the commission concerning the different points presented in the first part. (J.S.)

  20. Elevated Expression of KiSS-1 in Placenta of Chinese Women with Early-Onset Preeclampsia

    Science.gov (United States)

    Qiao, Chong; Wang, Chunhui; Zhao, Jiao; Liu, Caixia; Shang, Tao

    2012-01-01

    Preeclampsia (PE) is a heterogeneous syndrome affecting 2% to 8% of all pregnancies and is the world’s leading cause of fetal and maternal morbidity and mortality. In many cases of PE, shallow trophoblast invasion results in inappropriate maternal spiral artery remodeling and impaired placental function. Multiple genes have been implicated in trophoblast invasion, among which are KiSS-1 and GPR54. The gene product of KiSS-1 is metastin, which is a ligand for the receptor GPR54. Both metastin and GPR54 are expressed in the placenta of normal pregnancy and have been implicated in modulating trophoblast invasion through inhibiting migration of trophoblast cells. We have previously reported that the expression level of KiSS-1 was higher in trophoblasts from women with preeclampsia as compared to normal controls. Here, using quantitative RT-PCR, Western blot analysis and immunohistochemistry, we extend our analysis to demonstrate that elevated KiSS-1 expression occurs only in early-onset preeclampsia (ePE) and not late-onset preeclampsia (lPE). However, no difference in the expression levels of GPR54 is observed between ePE, lPE, and normal controls. Further, we show that KiSS-1 expression is also increased in placenta of intrauterine death and birth asphyxia in comparison to normal newborns of ePE and lPE. Our findings suggest that aberrant upregulation of KiSS-1 expression may contribute to the underlying mechanism of ePE as well as birth asphyxia. PMID:23145030

  1. Elevated expression of KiSS-1 in placenta of Chinese women with early-onset preeclampsia.

    Directory of Open Access Journals (Sweden)

    Chong Qiao

    Full Text Available Preeclampsia (PE is a heterogeneous syndrome affecting 2% to 8% of all pregnancies and is the world's leading cause of fetal and maternal morbidity and mortality. In many cases of PE, shallow trophoblast invasion results in inappropriate maternal spiral artery remodeling and impaired placental function. Multiple genes have been implicated in trophoblast invasion, among which are KiSS-1 and GPR54. The gene product of KiSS-1 is metastin, which is a ligand for the receptor GPR54. Both metastin and GPR54 are expressed in the placenta of normal pregnancy and have been implicated in modulating trophoblast invasion through inhibiting migration of trophoblast cells. We have previously reported that the expression level of KiSS-1 was higher in trophoblasts from women with preeclampsia as compared to normal controls. Here, using quantitative RT-PCR, Western blot analysis and immunohistochemistry, we extend our analysis to demonstrate that elevated KiSS-1 expression occurs only in early-onset preeclampsia (ePE and not late-onset preeclampsia (lPE. However, no difference in the expression levels of GPR54 is observed between ePE, lPE, and normal controls. Further, we show that KiSS-1 expression is also increased in placenta of intrauterine death and birth asphyxia in comparison to normal newborns of ePE and lPE. Our findings suggest that aberrant upregulation of KiSS-1 expression may contribute to the underlying mechanism of ePE as well as birth asphyxia.

  2. Antibacterial effects, biocompatibility and electrochemical behavior of zinc incorporated niobium oxide coating on 316L SS for biomedical applications

    Science.gov (United States)

    Pradeep PremKumar, K.; Duraipandy, N.; Manikantan Syamala, Kiran; Rajendran, N.

    2018-01-01

    In the present study, Nb2O5 (NZ0) composite coatings with various concentrations of zinc (NZ2, NZ4 & NZ6) are produced on 316L SS by sol-gel method with the aim of improving its antibacterial activity, bone formability and corrosion resistance properties. This work studied the surface characterization of NZ0, NZ2, NZ4 & NZ6 coated 316L SS by ATR-FTIR, XRD, HR-SEM with EDAX. The synthesized coatings were different in the morphological aspects, NZ0 shows mesoporous morphology whereas irregular cluster like morphology was observed for the zinc incorporated coatings. The chemical composition of the NZ0 and NZ4 composite coatings were studied by XPS and the results revealed that the zinc exist as ZnO and Nb as Nb2O5 in the coatings. The increase in the concentration of zinc in Nb2O5 increases the hydrophilic nature identified by water contact angle studies. The potentiodynamic polarization studies in simulated body fluid reveals the increase in polarization resistance with decrease in current density (icorr) and electrochemical impedance spectroscopic studies with increase in charge transfer resistance (Rct) and double layer capacitance (Qdl) were observed for NZ4 coated 316L SS. The inhibition of Staphylococcus aureus and Escherichia coli bacteria were identified for NZ4 coated 316L SS by bacterial viability studies. The NZ4 coated 316L SS showed better Osseo-integration by spreading the MG 63 osteoblast cells. The study results imply that zinc incorporated Nb2O5 (NZ4) composite coating exhibits antibacterial activity and also enhance the corrosion resistance and biocompatibility of the 316L SS.

  3. Structure and Cellular Dynamics of Deinococcus radiodurans Single-stranded DNA (ssDNA)-binding Protein (SSB)-DNA Complexes*

    Science.gov (United States)

    George, Nicholas P.; Ngo, Khanh V.; Chitteni-Pattu, Sindhu; Norais, Cédric A.; Battista, John R.; Cox, Michael M.; Keck, James L.

    2012-01-01

    The single-stranded DNA (ssDNA)-binding protein from the radiation-resistant bacterium Deinococcus radiodurans (DrSSB) functions as a homodimer in which each monomer contains two oligonucleotide-binding (OB) domains. This arrangement is exceedingly rare among bacterial SSBs, which typically form homotetramers of single-OB domain subunits. To better understand how this unusual structure influences the DNA binding and biological functions of DrSSB in D. radiodurans radiation resistance, we have examined the structure of DrSSB in complex with ssDNA and the DNA damage-dependent cellular dynamics of DrSSB. The x-ray crystal structure of the DrSSB-ssDNA complex shows that ssDNA binds to surfaces of DrSSB that are analogous to those mapped in homotetrameric SSBs, although there are distinct contacts in DrSSB that mediate species-specific ssDNA binding. Observations by electron microscopy reveal two salt-dependent ssDNA-binding modes for DrSSB that strongly resemble those of the homotetrameric Escherichia coli SSB, further supporting a shared overall DNA binding mechanism between the two classes of bacterial SSBs. In vivo, DrSSB levels are heavily induced following exposure to ionizing radiation. This accumulation is accompanied by dramatic time-dependent DrSSB cellular dynamics in which a single nucleoid-centric focus of DrSSB is observed within 1 h of irradiation but is dispersed by 3 h after irradiation. These kinetics parallel those of D. radiodurans postirradiation genome reconstitution, suggesting that DrSSB dynamics could play important organizational roles in DNA repair. PMID:22570477

  4. Fast bilateral breast coverage with high spectral and spatial resolution (HiSS) MRI at 3T.

    Science.gov (United States)

    Medved, Milica; Li, Hui; Abe, Hiroyuki; Sheth, Deepa; Newstead, Gillian M; Olopade, Olufunmilayo I; Giger, Maryellen L; Karczmar, Gregory S

    2017-11-01

    To develop and assess a full-coverage, sensitivity encoding (SENSE)-accelerated breast high spatial and spectral resolution (HiSS) magnetic resonance imaging (MRI) within clinically reasonable times as a potential nonenhanced MRI protocol for breast density measurement or breast cancer screening. Sixteen women with biopsy-proven cancer or suspicious lesions, and 13 women who were healthy volunteers or were screened for breast cancer, received 3T breast MRI exams, including SENSE-accelerated HiSS MRI, which was implemented as a submillimeter spatial resolution echo-planar spectroscopic imaging (EPSI) sequence. In postprocessing, fat and water resonance peak height and integral images were generated from EPSI data. The postprocessing software was custom-designed, and new algorithms were developed to enable processing of whole-coverage axial HiSS datasets. Water peak height HiSS images were compared to pre- and postcontrast T 1 -weighted images. Fat suppression was quantified as parenchymal-to-suppressed-fat signal ratio in HiSS water peak height and nonenhanced T 1 -weighted images, and artifact levels were scored. Approximately a 4-fold decrease in acquisition speed, with a concurrent 2.5-fold decrease in voxel size, was achieved, with low artifact levels, and with spectral signal-to-noise ratio (SNR) of 45:1. Fat suppression was 1.9 times more effective (P SENSE-accelerated breast HiSS MRI within clinically reasonable times, as a potential protocol for breast density measurement or breast cancer screening. 2 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2017;46:1341-1348. © 2017 International Society for Magnetic Resonance in Medicine.

  5. Investigating the presence of predatory bacteria on algal bloom samples using a T6SS gene marker.

    Science.gov (United States)

    Hendricks, J.; Sison-Mangus, M.; Mehic, S.; McMahon, E.

    2015-12-01

    Predation is considered to be a major driving force in evolution and ecology, which has been observed affecting individual organisms, communities, and entire ecosystems. The type VI secretion system (T6SS) is an intermembranal protein complex identified in certain bacteria, which appears to have evolved strictly as a mechanism of predation. The effects of bacteria on phytoplankton physiology are still understudied, however, studies have shown that the interactions between bacteria that inhabit the phycosphere of phytoplankton can possibly result in coevolution of native host and microbiota. It is unclear if bacteria can prey upon other bacteria to gain advantages during periods of high phytoplankton density. Here, we investigate the predatory interactions between bacteria and analyze environmental samples for the presence of predatory bacterial genes in an effort to understand bacteria-bacteria and phytoplankton interactions during algal blooms. DNA were extracted from bacterial samples collected weekly from size-fractionated samples using 3.0 um and 0.2 um membrane filters at the Santa Cruz wharf. PCR amplification and gel visualization for the presence of T6SS gene was carried out on bloom and non-bloom samples. Moreover, we carried out a lab- based experiment to observe bacteria-bacteria interaction that may hint for the presence of predatory behavior between bacterial taxa. We observed what appeared to be a predatory biofilm formation between certain bacterial species. These bacteria, however, did not contain the T6SS genes. On the contrary the T6SS gene was discovered in some of the bloom samples gathered from the Santa Cruz wharf. It is still unclear if the predatory mechanisms facilitate the abundance of certain groups of bacteria that contain the T6SS genes during algal blooms, but our evidence suggest that bacterial predation through T6SS mechanism is present during bloom events.

  6. SS-mPEG chemical modification of recombinant phospholipase C for enhanced thermal stability and catalytic efficiency.

    Science.gov (United States)

    Fang, Xian; Wang, Xueting; Li, Guiling; Zeng, Jun; Li, Jian; Liu, Jingwen

    2018-01-25

    PEGylation is one of the most promising and extensively studied strategies for improving the properties of proteins as well as enzymic physical and thermal stability. Phospholipase C, hydrolyzing the phospholipids offers tremendous applications in diverse fields. However, the poor thermal stability and higher cost of production have restricted its industrial application. This study focused on improving the stabilization of recombinant PLC by chemical modification with methoxypolyethylene glycol-Succinimidyl Succinate (SS-mPEG, MW 5000). PLC gene from isolate Bacillus cereus HSL3 was fused with SUMO, a novel small ubiquitin-related modifier expression vector and over expressed in Escherichia coli. The soluble fraction of SUMO-PLC reached 80% of the total recombinant protein. The enzyme exhibited maximum catalytic activity at 80 °C and was relatively thermostable at 40-70 °C. It showed extensive substrate specificity pattern and marked activity toward phosphatidylcholine, which made it a typical non-specific PLC for industrial purpose. SS-mPEG-PLC complex exhibited an enhanced thermal stability at 70-80 °C and the catalytic efficiency (Kcat/Km) had increased by 3.03 folds compared with free PLC. CD spectrum of SS-mPEG-PLC indicated a possible enzyme aggregation after chemical modification, which contributed to the higher thermostability of SS-mPEG-PLC. The increase of antiparallel β sheets in secondary structure also made it more stable than parallel β sheets. The presence of SS-mPEG chains on the enzyme molecule surface somewhat changed the binding rate of the substrates, leading to a significant improvement in catalytic efficiency. This study provided an insight into the addition of SS-mPEG for enhancing the industrial applications of phospholipase C at higher temperature. Copyright © 2018 Elsevier B.V. All rights reserved.

  7. Computational imaging analysis of fibrin matrices with the inclusion of erythrocytes from homozygous SS blood reveals agglomerated and amorphous structures.

    Science.gov (United States)

    Averett, Rodney D; Norton, David G; Fan, Natalie K; Platt, Manu O

    2017-01-01

    Sickle cell disease is a single point mutation disease that is known to alter the coagulation system, leading to hypercoagulable plasma conditions. These hypercoagulable conditions can lead to complications in the vasculature, caused by fibrin clots that form undesirably. There is a need to understand the morphology and structure of fibrin clots from patients with sickle cell disease, as this could lead to further discovery of treatments and life-saving therapies. In this work, a computational imaging analysis method is presented to evaluate fibrin agglomeration in the presence of erythrocytes (RBCs) homozygous for the sickle cell mutation (SS). Numerical algorithms were used to determine agglomeration of fibrin fibers within a matrix with SS RBCs to test the hypothesis that fibrin matrices with the inclusion of SS RBCs possess a more agglomerated structure than native fibrin matrices with AA RBCs. The numerical results showed that fibrin structures with SS RBCs displayed an overall higher degree of agglomeration as compared to native fibrin structures. The computational algorithm was also used to evaluate fibrin fiber overlap (aggregation) and anisotropy (orientation) in normal fibrin matrices compared to fibrin matrices polymerized around SS RBCs; however, there was no statistical difference. Ultrasound measurements of stiffness revealed rigid RBCs in the case of samples derived from homozygous SS blood, and densely evolving matrices, when compared to normal fibrin with the inclusion of AA RBCs. An agglomeration model is suggested to quantify the fibrin aggregation/clustering near RBCs for both normal fibrin matrices and for the altered structures. The results of this work are important in the sense that the understanding of aggregation and morphology in fibrin clots with incorporation of RBCs from persons living with sickle cell anemia may elucidate the complexities of comorbidities and other disease complications.

  8. Valmentavan johtajuuden toteutuminen ja sen kehittäminen tiimityössä - case Savonia-ammattikorkeakoulu

    OpenAIRE

    Mustonen, Marjo

    2017-01-01

    Työelämä muuttuu jatkuvasti, asiantuntijaorganisaatiot lisääntyvät ja tiimiorganisaatioissa vastuuta on jaettu enenevässä määrin tiimeille. Tämä asettaa myös johtamiselle uusia haasteita ja yhtenä ratkaisuna siihen pidetään valmentavaa johtajuutta. Tämän opinnäytetyön lähtökohtana oli tutkia, miten valmentava johtajuus näkyy ja miten sen periaatteet toteutuvat Savonia-ammattikorkeakoulun tiimityössä ja sen johtamisessa. Samalla selvitettiin tunnetaanko valmentavan johtajuuden käsite ja sen pe...

  9. Intracranial Aneurysms in Sickle-Cell Disease Are Associated With the Hemoglobin SS Genotype But Not With Moyamoya Syndrome

    DEFF Research Database (Denmark)

    Birkeland, Peter; Gardner, Kate; Kesse-Adu, Rachel

    2016-01-01

    BACKGROUND AND PURPOSE: Intracranial aneurysms and aneurysmal subarachnoid hemorrhage may occur more frequently in sickle-cell disease (SCD), and this could be related to the sickle genotype and moyamoya syndrome seen in SCD. METHODS: Records from a total of 1002 patients with SCD attending 2...... had imaging data; the prevalence was significantly higher in patients with HbSS genotype compared with other sickle genotypes with the highest prevalence (15%) observed in women in the age group 30 to 39 years. Fifty-one HbSS patients had a moyamoya vasculopathy, but only 3 of these had concomitant...

  10. Opas henkilökunnalle selkäleikkauspotilaan asentohoidosta heräämössä

    OpenAIRE

    Michelsson, Patrick; Bellaveglia, Laura

    2016-01-01

    Opinnäytetyömme tarkoitus oli tuottaa opas selkäleikkauspotilaan asentohoidon toteuttamisesta Jorvin sairaalan leikkaus- ja anestesiaosaston heräämön henkilökunnalle. Oppaan tarkoituksena on koota ydinasiat selkäleikkauspotilaan hyvän asentohoidon kannalta. Opinnäytetyön tavoitteena on kehittää selkäleikkauspotilaiden asentohoitoa heräämössä. Opinnäytetyö tehtiin osana Laurea-ammattikorkeakoulun ja HUS:n välistä Ohjaus hoitotyössä -hanketta. Toive opinnäytetyöaiheesta tuli Jorvin sairaalan le...

  11. Constraints on particle acceleration in SS433/W50 from MAGIC and H.E.S.S. observations

    OpenAIRE

    MAGIC Collaboration; Ahnen, M. L.; Ansoldi, S.; Antonelli, L. A.; Arcaro, C.; Babić, A.; B. Banerjee(Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Salt Lake, Sector-1, Kolkata 700064, India); P. Bangale(Max-Planck-Institut für Physik, D-80805 München, Germany); de Almeida, U. Barres; Barrio, J. A.; González, J. Becerra; Bednarek, W.; Bernardini, E.; Berti, A; B. Biasuzzi(b)

    2017-01-01

    The large jet kinetic power and non-thermal processes occurring in the microquasar SS 433 make this source a good candidate for a very high-energy (VHE) gamma-ray emitter. Gamma-ray fluxes have been predicted for both the central binary and the interaction regions between jets and surrounding nebula. Also, non-thermal emission at lower energies has been previously reported. We explore the capability of SS 433 to emit VHE gamma rays during periods in which the expected flux attenuation due to ...

  12. Facebook-yhteisöpalvelun hyödyntäminen yrityksen markkinointiviestinnässä

    OpenAIRE

    Pajamäki, Ida

    2014-01-01

    Tämän opinnäytetyön tavoitteena oli selvittää, miksi yritysten olisi kannattavaa olla Facebook-yhteisöpalvelussa ja millaisia keinoja niillä on käytössään Facebook-palvelussa markkinoidessaan. Opinnäytetyössä rakennettiin työn toimeksiantajalle, Mainostoimisto Gurulle, oma Facebook-sivu. Guru on Turun ammattikorkeakoulun sisäinen mainostoimisto, jossa suunnitellaan kaikenlaista markkinointiviestintää opiskelijavoimin. Teoriaosassa käsiteltiin lyhyesti sosiaalista mediaa ja Facebook-palvel...

  13. A Disability Studies in Education Analysis of the edTPA through Teacher Candidate Perspectives

    Science.gov (United States)

    Bacon, Jessica; Blachman, Sheila

    2017-01-01

    This analysis of the Special Education edTPA is written by two professors who co-taught a student teaching seminar at one institution and supported the first groups of teacher candidates required to submit the edTPA for certification in New York State. Data were gathered over three semesters and included open-ended student surveys, student…

  14. Multiple Intelligence and Digital Learning Awareness of Prospective B.Ed Teachers

    Science.gov (United States)

    Gracious, F. L. Antony; Shyla, F. L. Jasmine Anne

    2012-01-01

    The present study Multiple Intelligence and Digital Learning Awareness of prospective B.Ed teachers was probed to find the relationship between Multiple Intelligence and Digital Learning Awareness of Prospective B.Ed Teachers. Data for the study were collected using self made Multiple Intelligence Inventory and Digital Learning Awareness Scale.…

  15. A Review of "Older, Wiser, Sexually Smarter: 30 Sex Ed Lessons for Adults Only"

    Science.gov (United States)

    Garrity, Joan Mogul

    2010-01-01

    While virtually all sex ed curricula are designed to be used with children, teens and young adults, "Older, Wiser, Sexually Smarter: 30 Sex Ed Lessons for Adults Only" ([C] 2009, Planned Parenthood of Greater Northern New Jersey) offers lessons to help participants fully embrace the possibility of sexual pleasure and intimacy from mid-life through…

  16. Is There a Future for Teacher Ed Curriculum? An Answer from History and Moral Philosophy

    Science.gov (United States)

    Null, J. Wesley

    2008-01-01

    Is there a future for teacher ed "curriculum"? The author contends that he is not sure if there is a future for teacher ed curriculum, but if such a future is to exist, the answer will come only from history and moral philosophy. In this article, the author opines that individuals cannot make good decisions about the future of teacher ed…

  17. Does routine symptom screening with ESAS decrease ED visits in breast cancer patients undergoing adjuvant chemotherapy?

    Science.gov (United States)

    Barbera, L; Sutradhar, R; Howell, D; Sussman, J; Seow, H; Dudgeon, D; Atzema, C; Earle, C; Husain, A; Liu, Y; Krzyzanowska, M K

    2015-10-01

    In 2007, the provincial cancer agency in Ontario, Canada initiated a wide-scale program to screen for symptoms in the cancer population using the Edmonton Symptom Assessment Scale (ESAS). The purpose of this study is to evaluate the impact of screening with ESAS on emergency department (ED) visit rates in women with breast cancer receiving adjuvant chemotherapy. This retrospective cohort study used linked administrative health care data from across the province of Ontario, Canada. The cohort included all women aged ≥18 who were diagnosed with stage I-III breast cancer between January 2007 and December 2009 and received adjuvant chemotherapy within 6 months of diagnosis. Using an adjusted recurrent event model, we examined the association of screening with ESAS at a clinic visit on the ED visit rate. The relative rate of ED visits was 0.57 when prior ESAS screening occurred compared to when it did not. The relative rate of ED visits was 0.83 when the prior number of ESAS screens was modeled as a continuous variable. Alternatively stated, the rate of ED visits was 43 % lower among patients previously screened with ESAS compared to those not previously screened. For each additional prior ESAS assessment, there was a 17 % decreased rate of ED visits. Our results demonstrate that screening with ESAS is associated with decreased ED visits. To our knowledge, this is the first report on the effectiveness of routinely documenting a patient reported outcome on ED visits, in a real-world setting.

  18. Book Review: John M. Hobson and Leonard Seabrooke (2007) (eds) Everyday Politics of the World Economy

    DEFF Research Database (Denmark)

    Strange, Michael Stewart

    2009-01-01

    Book Review: John M. Hobson and Leonard Seabrooke (2007) (eds) Everyday Politics of the World Economy (Cambridge: Cambridge University Press), 254pp. Udgivelsesdato: 2009......Book Review: John M. Hobson and Leonard Seabrooke (2007) (eds) Everyday Politics of the World Economy (Cambridge: Cambridge University Press), 254pp. Udgivelsesdato: 2009...

  19. Mental Health and Academic Achievement among M.Ed. Students in Kerala

    Science.gov (United States)

    Moradi Sheykhjan, Tohid; K., Rajeswari; Jabari, Kamran

    2017-01-01

    The present research endeavor was aimed to assess relationship between Mental Health and Academic Achievement among M.Ed. students in Kerala. The sample of the study consisted of 314 M.Ed. students in Kerala. The method used for the present study was survey method. Mental Health Status Scale (M.H.S. Scale) was used and the study used the total…

  20. Agro obacter pop rium m pular In mediate ndica r ed gen rice Ra ...

    African Journals Online (AJOL)

    SAM

    ed equally. e and Skoog, 2 remain perma e mediate ndica r. ¥, Swadesh. Plant Patholog unge Circular ge, 1/1, Suren hutosh Colleg ceived 27 Januar ansformation ing a binary ed. Various p. , bacterial co nd the conce equence an requency we ormation tech m tumefacien ry food crop population, h and proteom two third of t.

  1. Connecting Teachers and Ed-Tech Developers: Lessons from NYC's "Gap App" Program. Technical Appendices

    Science.gov (United States)

    Villavicencio, Adriana; Siman, Nina; Lafayette, Camille; Kang, David

    2016-01-01

    In 2011, with support from a federal Investing in Innovation grant, the NYC Department of Education launched Innovate NYC Schools. The initiative was designed to address two, related challenges to effectively integrating education technology (ed-tech) into classrooms: First, procurement of ed-tech tools is often hampered by a disconnect between…

  2. Connecting Teachers and Ed-Tech Developers: Lessons from NYC's "Gap App" Program. Report

    Science.gov (United States)

    Villavicencio, Adriana; Siman, Nina; Lafayette, Camille; Kang, David

    2016-01-01

    In 2011, with support from a federal Investing in Innovation grant, the NYC Department of Education launched Innovate NYC Schools. The initiative was designed to address two, related challenges to effectively integrating education technology (ed-tech) into classrooms: First, procurement of ed-tech tools is often hampered by a disconnect between…

  3. A mechanistic nitrogen limitation model for CLM(ED)

    Science.gov (United States)

    Ali, A. A.; Xu, C.; McDowell, N. G.; Rogers, A.; Wullschleger, S. D.; Fisher, R.; Vrugt, J. A.

    2014-12-01

    Photosynthetic capacity is a key plant trait that determines the rate of photosynthesis; however, in Earth System Models it is either a fixed value or derived from a linear function of leaf nitrogen content. A mechanistic leaf nitrogen allocation model have been developed for a DOE-sponsored Community Land Model coupled to the Ecosystem Demography model (CLM-ED) to predict the photosynthetic capacity [Vc,max25 (μmol CO2 m-2 s-1)] under different environmental conditions at the global scale. We collected more than 800 data points of photosynthetic capacity (Vc,max25) for 124 species from 57 studies with the corresponding leaf nitrogen content and environmental conditions (temperature, radiation, humidity and day length) from literature and the NGEE arctic site (Barrow). Based on the data, we found that environmental control of Vc,max25 is about 4 times stronger than the leaf nitrogen content. Using the Markov-Chain Monte Carlo simulation approach, we fitted the collected data to our newly developed nitrogen allocation model, which predict the leaf nitrogen investment in different components including structure, storage, respiration, light capture, carboxylation and electron transport at different environmental conditions. Our results showed that our nitrogen allocation model explained 52% of variance in observed Vc,max25 and 65% variance in observed Jmax25 using a single set of fitted model parameters for all species. Across the growing season, we found that the modeled Vc,max25 explained 49% of the variability in measured Vc,max25. In the context of future global warming, our model predicts that a temperature increase by 5oC and the doubling of atmospheric carbon dioxide reduced the Vc,max25 by 5%, 11%, respectively.

  4. Francesco Fiorentino (ed., Figure e forme della memoria culturale

    Directory of Open Access Journals (Sweden)

    Paola Di Gennaro

    2012-12-01

    Full Text Available Lavorare la memoria, obliare, ricordare, riscrivere il passato con gli strumenti del presente, è questo il soggetto e l’oggetto dei saggi riuniti in questo volume da Francesco Fiorentino, che li introduce attraverso l’operazione artistica di Robert Rauschenberg, il quale, dopo aver chiesto un disegno a de Kooning, maestro dell’Espressionismo astratto, lo espone cancellato. Questa «sottile meditazione iconologica sul tempo edipico dell’arte moderna» (7-8 annuncia uno dei leitmotiv del volume, verità ovvia ma necessaria: se la novità ha valore estetico, come è vero dal Settecento, allora il passato diventa fondamentale come sistema interno dell’arte. È su questo necessario filtro di selezione che agiscono le memorie citate nei saggi seguenti: intersezioni di tracce e cancellazioni, di cui si indagano autenticità, costruzione, ricostruzione, senza che i confini siano tra l’altro mai definiti – ed è questo, forse, che crea l’arte, per suo mezzo e scopo, tramite l’inaffidabilità della memoria: arte che è, come il quadro di Rauschenberg, «rappresentazione della permanenza e, insieme, dell’inattingibilità di ciò che è cancellato» (11. È insomma la «dimensione performativa dell’agire culturale» che viene messa in evidenza in questo volume, memoria che è culturale, appunto, umana e storica, e che, come il Wunderblock di Freud, si cancella ma mai completamente, e mai innocuamente. L’irriconoscibilità finale del ricordo rappresenta quindi il risultato della dimenticanza, e il suo prodotto più creativo. È proprio l’infedeltà della memoria a renderla oggetto sostanziale dell’arte, così come di questo lavoro, oggetto autoriflessivo della memoria collettiva.

  5. The structure of the XPF-ssDNA complex underscores the distinct roles of the XPF and ERCC1 helix- hairpin-helix domains in ss/ds DNA recognition.

    Science.gov (United States)

    Das, Devashish; Folkers, Gert E; van Dijk, Marc; Jaspers, Nicolaas G J; Hoeijmakers, Jan H J; Kaptein, Robert; Boelens, Rolf

    2012-04-04

    Human XPF/ERCC1 is a structure-specific DNA endonuclease that nicks the damaged DNA strand at the 5' end during nucleotide excision repair. We determined the structure of the complex of the C-terminal domain of XPF with 10 nt ssDNA. A positively charged region within the second helix of the first HhH motif contacts the ssDNA phosphate backbone. One guanine base is flipped out of register and positioned in a pocket contacting residues from both HhH motifs of XPF. Comparison to other HhH-containing proteins indicates a one-residue deletion in the second HhH motif of XPF that has altered the hairpin conformation, thereby permitting ssDNA interactions. Previous nuclear magnetic resonance studies showed that ERCC1 in the XPF-ERCC1 heterodimer can bind dsDNA. Combining the two observations gives a model that underscores the asymmetry of the human XPF/ERCC1 heterodimer in binding at an ss/ds DNA junction. Copyright © 2012 Elsevier Ltd. All rights reserved.

  6. The structure of the XPF-ssDNA complex underscores the distinct roles of the XPF and ERCC1 helix- hairpin-helix domains in ss/ds DNA recognition

    NARCIS (Netherlands)

    Das, Devashish; Folkers, Gert; van Dijk, Marc; Jaspers, Nicolaas G.J.; Hoeijmakers, Jan H.J.; Kaptein, Robert; Boelens, Rolf

    2012-01-01

    Human XPF/ERCC1 is a structure-specific DNA endonuclease that nicks the damaged DNA strand at the 5' end during nucleotide excision repair. We determined the structure of the complex of the C-terminal domain of XPF with 10 nt ssDNA. A positively charged region within the second helix of the first

  7. Ääni virtuaalitodellisuudessa ja binauraalisen 3D-äänen vaikutus äänityössä

    OpenAIRE

    Silventoinen, Aki

    2015-01-01

    ssä opinnäytetyössä käsitellään virtuaalitodellisuutta yleisellä tasolla ja erityisesti äänisuunnittelun näkökulmasta. Tavoitteena on kehittää äänisuunnittelun malleja ensimmäisen persoonan virtuaalitodellisuussovelluksille. Opinnäytetyössä virtuaalitodellisuudella viitataan tekniikkaan, jossa ns. VR-lasien avulla “astutaan sisään” tietokonepohjaisesti luotuun keinotekoiseen, kolmiulotteiseen maailmaan. Työssä esitettäviä tutkimuskysymyksiä ovat: Onko binauraalisten 3D-äänten luominen s...

  8. Differential Ovarian Expression of KiSS-1 and GPR-54 During the Estrous Cycle and Photoperiod Induced Recrudescence in Siberian Hamsters (Phodopus sungorus)

    OpenAIRE

    Shahed, Asha; Young, Kelly A.

    2009-01-01

    Kisspeptins, coded by the KiSS-1 gene, regulate aspects of the reproductive axis by stimulating GnRH release via the G protein coupled receptor, GPR54. Recent reports show that KiSS/GPR54 may be key mediators in photoperiod-controlled reproduction in seasonal breeders, and that KiSS-1/GPR54 are expressed in the hypothalamus, ovaries, placenta, and pancreas. This study examined the expression of KiSS-1/GPR54 mRNA and protein in ovaries of Siberian hamsters (Phodopus sungorus). Ovaries from cyc...

  9. Ectopic overexpression of SsCBF1, a CRT/DRE-binding factor from the nightshade plant Solanum lycopersicoides, confers freezing and salt tolerance in transgenic Arabidopsis.

    Directory of Open Access Journals (Sweden)

    Lili Zhang

    Full Text Available The C-repeat (CRT/dehydration-responsive element (DRE binding factor (CBF/DREB1 transcription factors play a key role in cold response. However, the detailed roles of many plant CBFs are far from fully understood. A CBF gene (SsCBF1 was isolated from the cold-hardy plant Solanum lycopersicoides. A subcellular localization study using GFP fusion protein indicated that SsCBF1 is localized in the nucleus. We delimited the SsCBF1 transcriptional activation domain to the C-terminal segment comprising amino acid residues 193-228 (SsCBF1(193-228. The expression of SsCBF1 could be dramatically induced by cold, drought and high salinity. Transactivation assays in tobacco leaves revealed that SsCBF1 could specifically bind to the CRT cis-elements in vivo to activate the expression of downstream reporter genes. The ectopic overexpression of SsCBF1 conferred increased freezing and high-salinity tolerance and late flowering phenotype to transgenic Arabidopsis. RNA-sequencing data exhibited that a set of cold and salt stress responsive genes were up-regulated in transgenic Arabidopsis. Our results suggest that SsCBF1 behaves as a typical CBF to contribute to plant freezing tolerance. Increased resistance to high-salinity and late flowering phenotype derived from SsCBF1 OE lines lend more credence to the hypothesis that plant CBFs participate in diverse physiological and biochemical processes related to adverse conditions.

  10. Accuracy of ED Bedside Ultrasound for Identification of Gallstones: Retrospective Analysis of 575 Studies

    Directory of Open Access Journals (Sweden)

    Scruggs, William

    2008-01-01

    Full Text Available Study Objective: To determine the ability of emergency department (ED physicians to diagnose cholelithiasis with bedside ultrasound. Methods: ED gallbladder ultrasounds recorded over 37 months were compared to radiology ultrasound interpretation. Results: Of 1,690 ED gallbladder ultrasound scans performed during this period, radiology ultrasound was performed in 575/1690 (34% cases. ED physician bedside interpretation was 88% sensitive [95% CI, 84-91] and 87% specific [95% CI, 82-91], while positive predictive value (PPV was 91% [88- 94%] and negative predictive value (NPV was 83% [78-87%], using radiology interpretation as the criterion reference. Conclusion: ED physician ultrasound of the gallbladder for cholelithiasis is both sensitive and specific.

  11. High-Resolution Macromolecular Structure Determination by MicroED, a cryo-EM Method

    Science.gov (United States)

    Rodriguez, J.A.; Gonen, T.

    2017-01-01

    Microelectron diffraction (MicroED) is a new cryo-electron microscopy (cryo-EM) method capable of determining macromolecular structures at atomic resolution from vanishingly small 3D crystals. MicroED promises to solve atomic resolution structures from even the tiniest of crystals, less than a few hundred nanometers thick. MicroED complements frontier advances in crystallography and represents part of the rebirth of cryo-EM that is making macromolecular structure determination more accessible for all. Here we review the concept and practice of MicroED, for both the electron microscopist and crystallographer. Where other reviews have addressed specific details of the technique (Hattne et al., 2015; Shi et al., 2016; Shi, Nannenga, Iadanza, & Gonen, 2013), we aim to provide context and highlight important features that should be considered when performing a MicroED experiment. PMID:27572734

  12. The influence of sonority on the production of words ending in -ed by Brazilian EFL learners

    Directory of Open Access Journals (Sweden)

    Fernanda Delatorre

    2010-11-01

    Full Text Available This study investigated the influence of sonority on the production of vowel epenthesis in verbs ending in -ed by Brazilian learners of English. Participants were twenty-six upper-intermediate Brazilian EFL learners who read and audio-recorded ten paragraphs containing 91 verbs ending in ed. The results demonstrated that sonority influenced mispronunciations since the least sonorous obstruents preceding -ed induced more vowel epenthesis than the more sonorous sonorants, which in turn induced more epenthesis than vowels in the same position. The results also demonstrated that within the class of obstruents, affricates preceding -ed induced more epenthesis than stops, which induced more epenthesis than fricatives, and that, within the class of sonorants, nasals induced more epenthesis than liquids. These results suggest that markedness in terms of sonority affects the production of vowel epenthesis in words ending in -ed.

  13. The influence of sonority on the production of words ending in -ed by Brazilian EFL learners

    Directory of Open Access Journals (Sweden)

    Fernanda Delatorre

    2008-01-01

    Full Text Available http://dx.doi.org/10.5007/2175-8026.2008n55p83 This study investigated the influence of sonority on the production of vowel epenthesis in verbs ending in -ed by Brazilian learners of English. Participants were twenty-six upper-intermediate Brazilian EFL learners who read and audio-recorded ten paragraphs containing 91 verbs ending in ed. The results demonstrated that sonority influenced mispronunciations since the least sonorous obstruents preceding -ed induced more vowel epenthesis than the more sonorous sonorants, which in turn induced more epenthesis than vowels in the same position. The results also demonstrated that within the class of obstruents, affricates preceding -ed induced more epenthesis than stops, which induced more epenthesis than fricatives, and that, within the class of sonorants, nasals induced more epenthesis than liquids. These results suggest that markedness in terms of sonority affects the production of vowel epenthesis in words ending in -ed.

  14. Dynamic rockfall risk assessment along the SS113 coastal road (Northern Sicily)

    Science.gov (United States)

    Mastrolembo, V. Brunella; Penna, Ivanna; Voumard, Jérémie; Jaboyedoff, Michel

    2016-04-01

    Rockfalls are natural hazards that usually affect only small areas. However, due to the big amount of material that can be moved and the associated kinetic energy they can cost serious damages to infrastructures and people. Even fairly small rockfall fragments are a significant hazard if deposited on a highway or along a rail-road track since they are not easily detected and can cause accidents or derailments. Rockfalls can also cause the closure of streets resulting in long term indirect economic losses due to transportation delays as well as to the impact on the commercial and tourist activities. In letterature there are numerous examples of rockfall risk assessments along transportation corridors, most of them are based on the use of standard risk estimation methods. The latters are static approaches founded on a macroscopic view of road traffic, assumed as uniform in space and time, thus characterized by average values of parameters. Lately, a new dynamic approach has been developed within the 'risk analysis group' at the University of Lausanne (Voumard, 2013). It consists of a kinematic interpretation of road traffic where vehicles are parametrized as single entities with different characteristics, speed, dimensions and behaviour. We apply this new approach to estimate the dynamic risk due to rockfall occurrence on the SS113 national road running along the northern coast of Sicily. In this work we focus our attention on a ≈10 km section along which the SS113 road and a railway connect all the costal villages going through very steep cliffs and very close to the sea with evident problems of erosion and maintenance. The area is a tourist destination and many hotels and facilities are found along the road. Moreover the area was already hit in the past by numerous rockfalls resulting in the closure of the road for periods running from a few days up to a few years with big direct and indirect damages to the local socio-economic activities. In order to achieve a

  15. Pulsed laser deposition of zinc ferrite coatings on SS316 and characterization studies

    Energy Technology Data Exchange (ETDEWEB)

    Chandramohan, P.; Chandran, S.; Srinivasan, M.P.; Rangarajan, S.; Velmurugan, S.; Narasimhan, S.V. [BARC Facilities, Water and Steam Chemistry Div., Kalpakkam, Tamil Nadu (India); Krishnan, R.; Dash, S. [IGCAR, Materials Science Group, Thin Films and Coatings Section, Surface and Nanoscience Div., Kalpakkam, Tamil Nadu (India)

    2010-07-01

    Primary heat transport system in BWR and PWR releases mainly nickel and iron as the corrosion materials which get deposited on fuel clad and get activated in core and released to ex-core as Co-58 and Co-60 resulting in the incorporation of these on spinals leading to more than 90% activity. A non-radioactive element which is capable of reducing the corrosion release rate and minimize the crud deposits on fuel clad will help to bring down the surface contamination. In BWR and PWR, on PHT SS surfaces, the incorporation of Zn ions in ferrite lattice brings down corrosion rate and {sup 60}Co activity pick up considerably. Zinc, due to its higher stabilization energy, gets preferentially incorporated in the inner chromite layers compared with other elements and make the inner oxide layer more corrosion protective. An attempt has been made to prepare zinc ferrite (used as target) coating by pulse laser deposition technique on AISI 316 SS (substrate) using Nd-YAG laser (λ-1064nm; pulse width - 5 ns; pulse energy- 500 mJ; repetition rate - 10 Hz) at various temperatures ranging from 298K to 973K and at different pressures ranging from 10{sup -6} mbar to 10{sup -1}mbar. The synthesis of zinc ferrite coating by PLD causes a reduction in crystallite size. This is expected to help in better corrosion protection and reduced activity pick up. The coating thickness and surface roughness were measured using a surface profilometer and found to be ∼500nm and ∼40nm, respectively. The zinc ferrite coating prepared at 298K showed characteristic XRD pattern for pure zinc ferrite. The coating developed at higher temperatures showed a mixture of zinc ferrite and iron oxide phases. The Electrochemical impedance studies were carried out on these films at room temperature. Increase in the film deposition temperature shows decrease in the pore resistance R{sub p} and charge transfer resistance R{sub ct} of the films. Film formed with different pressure conditions show decrease in the

  16. ssHMM: extracting intuitive sequence-structure motifs from high-throughput RNA-binding protein data.

    Science.gov (United States)

    Heller, David; Krestel, Ralf; Ohler, Uwe; Vingron, Martin; Marsico, Annalisa

    2017-11-02

    RNA-binding proteins (RBPs) play an important role in RNA post-transcriptional regulation and recognize target RNAs via sequence-structure motifs. The extent to which RNA structure influences protein binding in the presence or absence of a sequence motif is still poorly understood. Existing RNA motif finders either take the structure of the RNA only partially into account, or employ models which are not directly interpretable as sequence-structure motifs. We developed ssHMM, an RNA motif finder based on a hidden Markov model (HMM) and Gibbs sampling which fully captures the relationship between RNA sequence and secondary structure preference of a given RBP. Compared to previous methods which output separate logos for sequence and structure, it directly produces a combined sequence-structure motif when trained on a large set of sequences. ssHMM's model is visualized intuitively as a graph and facilitates biological interpretation. ssHMM can be used to find novel bona fide sequence-structure motifs of uncharacterized RBPs, such as the one presented here for the YY1 protein. ssHMM reaches a high motif recovery rate on synthetic data, it recovers known RBP motifs from CLIP-Seq data, and scales linearly on the input size, being considerably faster than MEMERIS and RNAcontext on large datasets while being on par with GraphProt. It is freely available on Github and as a Docker image. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

  17. High Dietary Protein Exacerbates Hypertension and Renal Damage in Dahl SS Rats by Increasing Infiltrating Immune Cells in the Kidney

    National Research Council Canada - National Science Library

    De Miguel, Carmen; Lund, Hayley; Mattson, David L

    2011-01-01

    ...) compared to rats fed normal protein (132±3 mm Hg, 1.2±0.3) or low-protein (132±6 mm Hg, 0.3±0.1) diets. Significantly greater numbers of infiltrating T lymphocytes were observed in kidneys of SS rats fed the high-protein diet...

  18. A cerato-platanin protein SsCP1 targets plant PR1 and contributes to virulence of Sclerotinia sclerotiorum.

    Science.gov (United States)

    Yang, Guogen; Tang, Liguang; Gong, Yingdi; Xie, Jiatao; Fu, Yanping; Jiang, Daohong; Li, Guoqing; Collinge, David B; Chen, Weidong; Cheng, Jiasen

    2017-10-27

    Cerato-platanin proteins (CPs), which are secreted by filamentous fungi, are phytotoxic to host plants, but their functions have not been well defined to date. Here we characterized a CP (SsCP1) from the necrotrophic phytopathogen Sclerotinia sclerotiorum. Sscp1 transcripts accumulated during plant infection, and deletion of Sscp1 significantly reduced virulence. SsCP1 could induce significant cell death when expressed in Nicotiana benthamiana. Using yeast two-hybrid, GST pull-down, co-immunoprecipitation and bimolecular florescence complementation, we found that SsCP1 interacts with PR1 in the apoplast to facilitate infection by S. sclerotiorum. Overexpressing PR1 enhanced resistance to the wild-type strain, but not to the Sscp1 knockout strain of S. sclerotiorum. Sscp1-expressing transgenic plants showed increased concentrations of salicylic acid (SA) and higher levels of resistance to several plant pathogens (namely Botrytis cinerea, Alternaria brassicicola and Golovinomyces orontii). Our results suggest that SsCP1 is important for virulence of S. sclerotiorum and that it can be recognized by plants to trigger plant defense responses. Our results also suggest that the SA signaling pathway is involved in CP-mediated plant defense . © 2017 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University New Phytologist © 2017 New Phytologist Trust.

  19. Electrochemical and in vitro bioactivity of polypyrrole/ceramic nanocomposite coatings on 316L SS bio-implants.

    Science.gov (United States)

    Madhan Kumar, A; Nagarajan, S; Ramakrishna, Suresh; Sudhagar, P; Kang, Yong Soo; Kim, Hyongbum; Gasem, Zuhair M; Rajendran, N

    2014-10-01

    The present investigation describes the versatile fabrication and characterization of a novel composite coating that consists of polypyrrole (PPy) and Nb2O5 nanoparticles. Integration of the two materials is achieved by electrochemical deposition on 316L stainless steel (SS) from an aqueous solution of oxalic acid containing pyrrole and Nb2O5 nanoparticles. Fourier transform infrared spectral (FTIR) and X-ray diffraction (XRD) studies revealed that the existence of Nb2O5 nanoparticles in PPy matrix with hexagonal structure. Surface morphological analysis showed that the presence of Nb2O5 nanoparticles strongly influenced the surface nature of the nanocomposite coated 316L SS. Micro hardness results revealed the enhanced mechanical properties of PPy nanocomposite coated 316L SS due to the addition of Nb2O5 nanoparticles. The electrochemical studies were carried out using cyclic polarization and electrochemical impedance spectroscopy (EIS) measurements. In order to evaluate the biocompatibility, contact angle measurements and in vitro characterization were performed in simulated body fluid (SBF) and on MG63 osteoblast cells. The results showed that the nanocomposite coatings exhibit superior biocompatibility and enhanced corrosion protection performance over 316L SS than pure PPy coatings. Copyright © 2014 Elsevier B.V. All rights reserved.

  20. An efficient method for recovery of target ssDNA based on amino-modified silica-coated magnetic nanoparticles.

    Science.gov (United States)

    Ashtari, Parviz; He, Xiaoxiao; Wang, Kemin; Gong, Ping

    2005-09-15

    In this paper, an improved recovery method for target ssDNA using amino-modified silica-coated magnetic nanoparticles (ASMNPs) is reported. This method takes advantages of the amino-modified silica-coated magnetic nanoparticles prepared using water-in-oil microemulsion technique, which employs amino-modified silica as the shell and iron oxide as the core of the magnetic nanoparticles. The nanoparticles have a silica surface with amino groups and can be conjugated with any desired bio-molecules through many existing amino group chemistry. In this research, a linear DNA probe was immobilized onto nanoparticles through streptavidin conjugation using covalent bonds. A target ssDNA(I) (5'-TMR-CGCATAGGGCCTCGTGATAC-3') has been successfully recovered from a crude sample under a magnet field through their special recognition and hybridization. A designed ssDNA fragment of severe acute respiratory syndrome (SARS) virus at a much lower concentration than the target ssDNA(I) was also recovered with high efficiency and good selectivity.

  1. Efficient Generation of Orthologous Point Mutations in Pigs via CRISPR-assisted ssODN-mediated Homology-directed Repair

    Directory of Open Access Journals (Sweden)

    Kankan Wang

    2016-01-01

    Full Text Available Precise genome editing in livestock is of great value for the fundamental investigation of disease modeling. However, genetically modified pigs carrying subtle point mutations were still seldom reported despite the rapid development of programmable endonucleases. Here, we attempt to investigate single-stranded oligonucleotides (ssODN mediated knockin by introducing two orthologous pathogenic mutations, p.E693G for Alzheimer's disease and p.G2019S for Parkinson's disease, into porcine APP and LRRK2 loci, respectively. Desirable homology-directed repair (HDR efficiency was achieved in porcine fetal fibroblasts (PFFs by optimizing the dosage and length of ssODN templates. Interestingly, incomplete HDR alleles harboring partial point mutations were observed in single-cell colonies, which indicate the complex mechanism of ssODN-mediated HDR. The effect of mutation-to-cut distance on incorporation rate was further analyzed by deep sequencing. We demonstrated that a mutation-to-cut distance of 11 bp resulted in a remarkable difference in HDR efficiency between two point mutations. Finally, we successfully obtained one cloned piglet harboring the orthologous p.C313Y mutation at the MSTN locus via somatic cell nuclear transfer (SCNT. Our proof-of-concept study demonstrated efficient ssODN-mediated incorporation of pathogenic point mutations in porcine somatic cells, thus facilitating further development of disease modeling and genetic breeding in pigs.

  2. [Story of three SS dentists during World War II: Pr Hugo Blaschke, Dr Hermann Pook and Dr Willy Frank].

    Science.gov (United States)

    Riaud, Xavier

    2006-12-01

    This story of three SS dentists shows very clearly that the medical code of ethics, under a totalitarian regime, ends where ideology begins. Professor Hugo Blaschke provided dental care to the most eminent Nazi leaders, but he also was the senior SS dentist. He was in charge of dental care in the Waffen-SS, and therefore, he had responsibility for the stocks of dental gold collected from the mouths of those who died in the concentration camps, in order to make dentures for his soldiers. Dr Hermann Pook was the dentist in charge of all the other dentists practising in the concentration camps. He was responsible for gathering statistics on the dental care provided for prisoners in the camps. His instructions were very clear: "No conservation or restorative treatment. Only extractions, and with no anaesthesia!" He was also in charge of gathering the gold that was collected in the camps, for the financial department of the SS. Dr Willy Frank, an Auschwitz dentist, took part in the selection of some of the convoys for the gas chambers. His participation in the collection of gold from the mouths of the dead was also established. These three men were sentenced to prison for War Crimes and Crimes against Humanity.

  3. Wehrmacht ja relva-SS - rahvaste sõpruse kool / Jüri Kotšinev

    Index Scriptorium Estoniae

    Kotšinev, Jüri, 1960-

    2009-01-01

    Teises maailmasõjas Saksa poolel sõdinud rahvastest, kelle hulgas oli palju NSV Liitu kuulunud rahvaid. Kasakavägedest Saksa sõjaväes ja belglasest Léon Degrellest, kellest sai enim austust pälvinud Euroopa Relva-SS vabatahtlik

  4. Comparative wood anatomy of Andromedeae s.s., Gaultherieae, Lyonieae and Oxydendreae (Vaccinioideae, Ericaceae s.l.)

    NARCIS (Netherlands)

    Lens, F.; Smets, E.; Jansen, S.

    2004-01-01

    The wood anatomical structure of 11 out of 13 genera from four tribes of the Vaccinioideae, namely Andromedeae s.s., Gaultherieae, Lyonieae and Oxydendreae (Ericaceae s.l.), is described using light and scanning electron microscopy. Several features of the secondary xylem support the tribal

  5. Identification of multi-locus genetic heterogeneity in anaplasma marginale ss. centrale and its restriction following tick-borne transmission.

    Science.gov (United States)

    Anaplasma marginale ss. centrale was the first vaccine used to protect against a rickettsial disease and continues in widespread use a century after initial implementation. As its use preceded development of either cryopreservation or cell culture, the vaccine strain was maintained for decades by se...

  6. Chemostat studies of bacteriophage M13 infected Escherichia coli JM109 for continuous ssDNA production.

    Science.gov (United States)

    Kick, Benjamin; Behler, Karl Lorenz; Severin, Timm Steffen; Weuster-Botz, Dirk

    2017-09-20

    Steady state studies in a chemostat enable the control of microbial growth rate at defined reaction conditions. The effects of bacteriophage M13 infection on maximum growth rate of Escherichia coli JM109 were studied in parallel operated chemostats on a milliliter-scale to analyze the steady state kinetics of phage production. The bacteriophage infection led to a decrease in maximum specific growth rate of 15% from 0.74h-1 to 0.63h-1. Under steady state conditions, a constant cell specific ssDNA formation rate of 0.15±0.004 mgssDNA gCDW-1h-1 was observed, which was independent of the growth rate. Using the estimated kinetic parameters for E. coli infected with bacteriophage M13, the ssDNA concentration in the steady state could be predicted as function of the dilution rate and the glucose concentration in the substrate. Scalability of milliliter-scale data was approved by steady state studies on a liter-scale at a selected dilution rate. An ssDNA space-time yield of 5.7mgL-1h-1 was achieved with increased glucose concentration in the feed at a dilution rate of 0.3h-1, which is comparable to established fed-batch fermentation with bacteriophage M13 for ssDNA production. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. KiSS1/GPR54 and estrogen-related gene expression profiles in primary breast cancer.

    Science.gov (United States)

    Jarząbek, Katarzyna; Kozłowski, Leszek; Milewski, Robert; Wołczyński, Sławomir

    2012-04-01

    The estrogen receptor α (ERα)-mediated pathway plays a critical role in breast cancer development and progression. KiSS1 was previously described as a metastasis suppressor gene in certain carcinomas. However, the role of KiSS1/GPR54 in breast cancer remains controversial. Whether the function of the KiSS1/GPR54 system depends on estrogen signaling in the breast cancer cell remains to be determined. This study aimed to determine the expression profiles of the KiSS1/GPR54, ERα, ERβ, aromatase and cyclin D1 genes in human breast cancer tissues, and to identify a possible link between the expression levels of the studied genes and the selected clinical and pathological features. The study subjects comprised 59 females treated surgically for primary breast cancer. Total RNA was extracted from frozen breast cancer tissues, and expression levels were examined to determine any correlations. We observed strong positive correlations between the expression levels of the studied genes. The expression of ERα correlated positively with progesterone receptors (PRs), and in these tumors we also observed positive correlations between KiSS1, GPR54 and cyclin D1 mRNAs and the ERα protein. ER-positive breast tumors exhibited higher KiSS1 and GPR54 levels than the ER-negative tumors. The expression levels of the ERα and GPR54 transcripts were higher in the moderately differentiated tumors (G2) compared to the poorly differentiated high-grade (G3) cancers. We also found that HER-2/neu status in breast cancer is negatively associated with GPR54 mRNA expression. Decreasing GPR54 mRNA expression levels in HER-2/neu (+) tumors may be associated with the deregulation of the classical estrogen-mediated signaling pathway in breast tumors, and therefore, with promotion of tumor invasiveness. Our findings indicate that genes involved in the KiSS1/GPR54 system, as well as in the estrogen signaling pathway, may be utilizable molecular factors in pathogenesis studies of breast cancer.

  8. Structure of the hDmc1-ssDNA filament reveals the principles of its architecture.

    Directory of Open Access Journals (Sweden)

    Andrei L Okorokov

    Full Text Available In eukaryotes, meiotic recombination is a major source of genetic diversity, but its defects in humans lead to abnormalities such as Down's, Klinefelter's and other syndromes. Human Dmc1 (hDmc1, a RecA/Rad51 homologue, is a recombinase that plays a crucial role in faithful chromosome segregation during meiosis. The initial step of homologous recombination occurs when hDmc1 forms a filament on single-stranded (ss DNA. However the structure of this presynaptic complex filament for hDmc1 remains unknown. To compare hDmc1-ssDNA complexes to those known for the RecA/Rad51 family we have obtained electron microscopy (EM structures of hDmc1-ssDNA nucleoprotein filaments using single particle approach. The EM maps were analysed by docking crystal structures of Dmc1, Rad51, RadA, RecA and DNA. To fully characterise hDmc1-DNA complexes we have analysed their organisation in the presence of Ca2+, Mg2+, ATP, AMP-PNP, ssDNA and dsDNA. The 3D EM structures of the hDmc1-ssDNA filaments allowed us to elucidate the principles of their internal architecture. Similar to the RecA/Rad51 family, hDmc1 forms helical filaments on ssDNA in two states: extended (active and compressed (inactive. However, in contrast to the RecA/Rad51 family, and the recently reported structure of hDmc1-double stranded (ds DNA nucleoprotein filaments, the extended (active state of the hDmc1 filament formed on ssDNA has nine protomers per helical turn, instead of the conventional six, resulting in one protomer covering two nucleotides instead of three. The control reconstruction of the hDmc1-dsDNA filament revealed 6.4 protein subunits per helical turn indicating that the filament organisation varies depending on the DNA templates. Our structural analysis has also revealed that the N-terminal domain of hDmc1 accomplishes its important role in complex formation through domain swapping between adjacent protomers, thus providing a mechanistic basis for coordinated action of hDmc1 protomers

  9. On the Nature of the Compact Object in SS 433. Observational Evidence of X-Ray Photon Index Saturation

    Science.gov (United States)

    Seifina, Elena; Titarchuk, Lev

    2010-01-01

    We present an analysis of the X-ray spectral properties observed from black hole , candidate (BHC) binary SS 433. We have analyzed Rossi X-ray Time Explorer (RXTE) data from this source, coordinated with Green Bank Interferometer/RATAN-600. We show that SS 433 undergoes a X-ray spectral transition from the low hard state (LHS) to the intermediate state (IS). We show that the X-ray broad-band energy spectra during all spectral states are well fit by a sum of so called "Bulk Motion Comptonization (BMC) component" and by two (broad and narrow) Gaussians for the continuum and line emissions respectively. In addition to these spectral model components we also find a strong feature that we identify as a" blackbody-like (BB)" component which color temperature is in the range of 4-5 keV in 24 IS spectra during the radio outburst decay in SS 433. Our observational results on the "high temperature BB" bump leads us to suggest the presence of gravitationally redshifted annihilation line emission in this source. In fact this spectral feature has been recently reproduced in Monte Carlo simulations by Laurent and Titarchuk. We have also established the photon index saturation at about 2.3 in index vs mass accretion correlation. This index-mass accretion correlation allows us to evaluate the low limit of black hole (BH) mass of compact object in SS 433, M(sub bh) approximately > 2 solar masses, using the scaling method using BHC GX 339-4 as a reference source. Our estimate of the BH mass in SS 433 is consistent with recent BH mass measurement using the radial-velocity measurements of the binary system by Hillwig & Gies who find that M(sub x)( = (4.3 +/- 0.8) solar masses. This is the smallest BH mass found up to now among all BH sources. Moreover, the index saturation effect versus mass accretion rate revealed in SS 433, like in a number of other BH candidates, is the strong observational evidence for the presence of a BH in SS 433.

  10. Burnout syndrome among dental students: a short version of the "Burnout Clinical Subtype Questionnaire" adapted for students (BCSQ-12-SS).

    Science.gov (United States)

    Montero-Marin, Jesus; Monticelli, Francesca; Casas, Marina; Roman, Amparo; Tomas, Inmaculada; Gili, Margarita; Garcia-Campayo, Javier

    2011-12-12

    Burnout has been traditionally defined in relation to the dimensions of "exhaustion", "cynicism", and "inefficiency". More recently, the Burnout Clinical Subtype Questionnaire (BCSQ-12) further established three different subtypes of burnout: the "frenetic" subtype (related to "overload"), the "under-challenged" subtype (related to "lack of development"), and the "worn-out" subtype (related to "neglect"). However, to date, these definitions have not been applied to students. The aims of this research were (1) to adapt a Spanish version of the BCSQ-12 for use with students, (2) to test its factorial validity, internal consistency, convergent and discriminant validity, and (3) to assess potential socio-demographic and occupational risk factors associated with the development of the subtypes. We used a cross-sectional design on a sample of dental students (n = 314) from Santiago and Huesca universities (Spain). Participants completed the Burnout Clinical Subtype Questionnaire Student Survey (BCSQ-12-SS), the Maslach Burnout Inventory Student Survey (MBI-SS), and a series of socio-demographic and occupational questions formulated for the specific purpose of this study. Data were subjected to exploratory factor analysis (EFA) using the principal component method with varimax orthogonal rotation. To assess the relations with the criterion, we calculated the Pearson correlation coefficient (r), multiple correlation coefficient (R(y.123)), and the coefficient of determination (R(2)(y.123)). To assess the association between the subtypes and the socio-demographic variables, we examined the adjusted odds ratio (OR) obtained from multivariate logistic regression models. Factorial analyses supported the theoretical proposition of the BCSQ-12-SS, with α-values exceeding 0.80 for all dimensions. The "overload-exhaustion" relation was r = 0.59 (p burnout as established by the BCSQ-12-SS. As such, the BCSQ-12-SS can be used for the recognition of clinical profiles and for the

  11. Mutation of SH2B3 (LNK), a GWAS candidate for hypertension, attenuates Dahl SS hypertension via inflammatory modulation

    Science.gov (United States)

    Rudemiller, Nathan P.; Lund, Hayley; Priestley, Jessica R. C.; Endres, Bradley T.; Prokop, Jeremy W.; Jacob, Howard J.; Geurts, Aron M.; Cohen, Eric P.; Mattson, David L.

    2015-01-01

    Human genome wide association studies (GWAS) have linked SH2B3 (LNK) to hypertension and renal disease, though little experimental investigation has been done to verify a role for SH2B3 in these pathologies. SH2B3, a member of the SH2B adaptor protein family, is an intracellular adaptor protein that functions as a negative regulator in many signaling pathways, including inflammatory signaling processes. To explore a mechanistic link between SH2B3 and hypertension, we targeted the SH2B3 gene for mutation on the Dahl salt-sensitive (SS) rat genetic background with zinc-finger nucleases (ZFN). The resulting mutation was a 6 base-pair, in-frame deletion within a highly-conserved region of the Src Homology 2 (SH2) domain of SH2B3. This mutation significantly attenuated Dahl salt-sensitive (SS) hypertension and renal disease. Also, infiltration of leukocytes into the kidneys, a key mediator of Dahl SS pathology, was significantly blunted in the Sh2b3em1Mcwi mutant rats. To determine if this was due to differences in immune signaling, bone marrow transplant studies were performed in which Dahl SS and Sh2b3em1Mcwi mutants underwent total body irradiation and were then transplanted with Dahl SS or Sh2b3em1Mcwi mutant bone marrow. Rats that received Sh2b3em1Mcwi mutant bone marrow had a significant reduction in mean arterial pressure and kidney injury when placed on a high salt diet (4% NaCl). These data further support a role for the immune system as a modulator of disease severity in the pathogenesis of hypertension and provide insight into inflammatory mechanisms at play in human hypertension and renal disease. PMID:25776069

  12. Burnout syndrome among dental students: a short version of the "Burnout Clinical Subtype Questionnaire" adapted for students (BCSQ-12-SS)

    Science.gov (United States)

    2011-01-01

    Background Burnout has been traditionally defined in relation to the dimensions of "exhaustion", "cynicism", and "inefficiency". More recently, the Burnout Clinical Subtype Questionnaire (BCSQ-12) further established three different subtypes of burnout: the "frenetic" subtype (related to "overload"), the "under-challenged" subtype (related to "lack of development"), and the "worn-out" subtype (related to "neglect"). However, to date, these definitions have not been applied to students. The aims of this research were (1) to adapt a Spanish version of the BCSQ-12 for use with students, (2) to test its factorial validity, internal consistency, convergent and discriminant validity, and (3) to assess potential socio-demographic and occupational risk factors associated with the development of the subtypes. Method We used a cross-sectional design on a sample of dental students (n = 314) from Santiago and Huesca universities (Spain). Participants completed the Burnout Clinical Subtype Questionnaire Student Survey (BCSQ-12-SS), the Maslach Burnout Inventory Student Survey (MBI-SS), and a series of socio-demographic and occupational questions formulated for the specific purpose of this study. Data were subjected to exploratory factor analysis (EFA) using the principal component method with varimax orthogonal rotation. To assess the relations with the criterion, we calculated the Pearson correlation coefficient (r), multiple correlation coefficient (Ry.123), and the coefficient of determination (R2y.123). To assess the association between the subtypes and the socio-demographic variables, we examined the adjusted odds ratio (OR) obtained from multivariate logistic regression models. Results Factorial analyses supported the theoretical proposition of the BCSQ-12-SS, with α-values exceeding 0.80 for all dimensions. The "overload-exhaustion" relation was r = 0.59 (p burnout as established by the BCSQ-12-SS. As such, the BCSQ-12-SS can be used for the recognition of clinical

  13. A novel silica nanotube reinforced ionic incorporated hydroxyapatite composite coating on polypyrrole coated 316L SS for implant application

    Energy Technology Data Exchange (ETDEWEB)

    Prem Ananth, K., E-mail: kpananth01@gmail.com [Department of Nanoscience and Technology, Bharathiar University, Coimbatore – 641 046 (India); Joseph Nathanael, A. [Department of Nano, Medical and Polymer Materials, Yeungnam University, Gyeongsan 712-749 (Korea, Republic of); Jose, Sujin P. [Department of Materials Science and Nano engineering, Rice University, Texas 77005 (United States); School of Physics, Madurai Kamaraj University, Madurai-625021 (India); Oh, Tae Hwan [Department of Nano, Medical and Polymer Materials, Yeungnam University, Gyeongsan 712-749 (Korea, Republic of); Mangalaraj, D. [Department of Nanoscience and Technology, Bharathiar University, Coimbatore – 641 046 (India)

    2016-02-01

    An attempt has been made to deposit a novel smart ion (Sr, Zn, Mg) substituted hydroxyapatite (I-HAp) and silica nanotube (SiNTs) composite coatings on polypyrrole (PPy) coated surgical grade 316L stainless steel (316L SS) to improve its biocompatibility and corrosion resistance. The I-HAp/SiNTS/PPy bilayer coating on 316L SS was prepared by electrophoretic deposition technique. Potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) studies were carried out. These results confirmed the significant improvement of the corrosion resistance of the 316L SS alloy by the I-HAp/SiNTs/PPy bilayer composite coating. The adhesion strength and hardness test confirmed the anticipated mechanical properties of the composite. A low contact angle value revealed the hydrophilic nature. Inductively coupled plasma-atomic emission spectroscopy (ICP-AES) was used for the leach out analysis of the samples. Added to this, the bioactivity of the composite was analyzed by observing the apatite formation in the SBF solution for 7, 14, 21 and 28 days of incubation. An enhancement of in vitro osteoblast attachment and cell viability was observed, which could lead to the optimistic orthopedic and dental applications. - Highlights: • Polypyrrole (PPy) coated 316L SS substrates were fabricated using electrodeposition method. • A novel silica nanotube (SiNTs) and ionic substituted (Sr, Zn, Mg) hydroxyapatite composite (I-HAp) were prepared. • The composite (I-HAp/SiNTs) was coated on PPy coated 316L SS substrate using electrophoretic deposition. • These results are favorable for corrosion resistance and enhanced osteoblast cell attachment for bone formation.

  14. Functional studies of ssDNA binding ability of MarR family protein TcaR from Staphylococcus epidermidis.

    Science.gov (United States)

    Chang, Yu-Ming; Chen, Cammy K-M; Chang, Yuan-Chih; Jeng, Wen-Yih; Hou, Ming-Hon; Wang, Andrew H-J

    2012-01-01

    The negative transcription regulator of the ica locus, TcaR, regulates proteins involved in the biosynthesis of poly-N-acetylglucosamine (PNAG). Absence of TcaR increases PNAG production and promotes biofilm formation in Staphylococci. Previously, the 3D structure of TcaR in its apo form and its complex structure with several antibiotics have been analyzed. However, the detailed mechanism of multiple antibiotic resistance regulator (MarR) family proteins such as TcaR is unclear and only restricted on the binding ability of double-strand DNA (dsDNA). Here we show by electrophoretic mobility shift assay (EMSA), electron microscopy (EM), circular dichroism (CD), and Biacore analysis that TcaR can interact strongly with single-stranded DNA (ssDNA), thereby identifying a new role in MarR family proteins. Moreover, we show that TcaR preferentially binds 33-mer ssDNA over double-stranded DNA and inhibits viral ssDNA replication. In contrast, such ssDNA binding properties were not observed for other MarR family protein and TetR family protein, suggesting that the results from our studies are not an artifact due to simple charge interactions between TcaR and ssDNA. Overall, these results suggest a novel role for TcaR in regulation of DNA replication. We anticipate that the results of this work will extend our understanding of MarR family protein and broaden the development of new therapeutic strategies for Staphylococci.

  15. Functional studies of ssDNA binding ability of MarR family protein TcaR from Staphylococcus epidermidis.

    Directory of Open Access Journals (Sweden)

    Yu-Ming Chang

    Full Text Available The negative transcription regulator of the ica locus, TcaR, regulates proteins involved in the biosynthesis of poly-N-acetylglucosamine (PNAG. Absence of TcaR increases PNAG production and promotes biofilm formation in Staphylococci. Previously, the 3D structure of TcaR in its apo form and its complex structure with several antibiotics have been analyzed. However, the detailed mechanism of multiple antibiotic resistance regulator (MarR family proteins such as TcaR is unclear and only restricted on the binding ability of double-strand DNA (dsDNA. Here we show by electrophoretic mobility shift assay (EMSA, electron microscopy (EM, circular dichroism (CD, and Biacore analysis that TcaR can interact strongly with single-stranded DNA (ssDNA, thereby identifying a new role in MarR family proteins. Moreover, we show that TcaR preferentially binds 33-mer ssDNA over double-stranded DNA and inhibits viral ssDNA replication. In contrast, such ssDNA binding properties were not observed for other MarR family protein and TetR family protein, suggesting that the results from our studies are not an artifact due to simple charge interactions between TcaR and ssDNA. Overall, these results suggest a novel role for TcaR in regulation of DNA replication. We anticipate that the results of this work will extend our understanding of MarR family protein and broaden the development of new therapeutic strategies for Staphylococci.

  16. High-Salt Intake Suppressed MicroRNA-133a Expression in Dahl SS Rat Myocardium

    Directory of Open Access Journals (Sweden)

    Tong-Shuai Guo

    2014-06-01

    Full Text Available Salt-sensitive individuals show earlier and more serious cardiac damage than nonsalt-sensitive ones. Some studies have suggested that microRNA-133a could reduce cardiac hypertrophy and myocardial fibrosis. The current study aims to investigate the different functions of high-salt intake on salt-sensitive (SS rats and Sprague-Dawley (SD rats and the involvement of microRNA-133a in these roles. After high-salt intervention, the left ventricular mass (LVW and left ventricular mass index (LVMI of the salt-sensitive high salt (SHS group were obviously higher than those of the salt-sensitive low salt (SLS group. However, the difference between the Sprague-Dawley high salt (DHS group and the Sprague-Dawley low salt (DLS group was not significant. Compared with SLS group, collagen I and connective tissue growth factor (CTGF in the heart of SHS group were significantly higher, whereas no statistical difference was observed between the DHS group and the DLS group. Compared with low-salt diet, microRNA-133a in the heart of both strains were significantly decreased, but that in the SHS group decreased more significantly. These results suggest that high salt intervention could down-regulate the expression of myocardial microRNA-133a, which may be one of the mechanisms involved in myocardial fibrosis in salt-sensitive hypertension.

  17. Anterior Chamber Angle Shape Analysis and Classification of Glaucoma in SS-OCT Images

    Directory of Open Access Journals (Sweden)

    Soe Ni Ni

    2014-01-01

    Full Text Available Optical coherence tomography is a high resolution, rapid, and noninvasive diagnostic tool for angle closure glaucoma. In this paper, we present a new strategy for the classification of the angle closure glaucoma using morphological shape analysis of the iridocorneal angle. The angle structure configuration is quantified by the following six features: (1 mean of the continuous measurement of the angle opening distance; (2 area of the trapezoidal profile of the iridocorneal angle centered at Schwalbe's line; (3 mean of the iris curvature from the extracted iris image; (4 complex shape descriptor, fractal dimension, to quantify the complexity, or changes of iridocorneal angle; (5 ellipticity moment shape descriptor; and (6 triangularity moment shape descriptor. Then, the fuzzy k nearest neighbor (fkNN classifier is utilized for classification of angle closure glaucoma. Two hundred and sixty-four swept source optical coherence tomography (SS-OCT images from 148 patients were analyzed in this study. From the experimental results, the fkNN reveals the best classification accuracy (99.11±0.76% and AUC (0.98±0.012 with the combination of fractal dimension and biometric parameters. It showed that the proposed approach has promising potential to become a computer aided diagnostic tool for angle closure glaucoma (ACG disease.

  18. ssDNA-Functionalized Nanoceria: A Redox-Active Aptaswitch for Biomolecular Recognition.

    Science.gov (United States)

    Bülbül, Gonca; Hayat, Akhtar; Andreescu, Silvana

    2016-04-06

    Quantification of biomolecular binding events is a critical step for the development of biorecognition assays for diagnostics and therapeutic applications. This paper reports the design of redox-active switches based on aptamer conjugated nanoceria for detection and quantification of biomolecular recognition. It is shown that the conformational transition state of the aptamer on nanoceria, combined with the redox properties of these particles can be used to create surface based structure switchable aptasensing platforms. Changes in the redox properties at the nanoceria surface upon binding of the ssDNA and its target analyte enables rapid and highly sensitive measurement of biomolecular interactions. This concept is demonstrated as a general applicable method to the colorimetric detection of DNA binding events. An example of a nanoceria aptaswitch for the colorimetric sensing of Ochratoxin A (OTA) and applicability to other targets is provided. The system can sensitively and selectivity detect as low as 0.15 × 10(-9) m OTA. This novel assay is simple in design and does not involve oligonucleotide labeling or elaborate nanoparticle modification steps. The proposed mechanism discovered here opens up a new way of designing optical sensing methods based on aptamer recognition. This approach can be broadly applicable to many bimolecular recognition processes and related applications. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  19. Retrospektive Bestimmung der elektromagnetischen Exposition durch analoge Rundfunksender im Rahmen von KiSS

    Directory of Open Access Journals (Sweden)

    J. Philipp

    2007-06-01

    Full Text Available Im Rahmen einer epidemiologischen Fall-Kontroll-Studie zur Untersuchung eines möglichen Zusammenhangs zwischen der Häufigkeit kindlicher Leukämien und elektromagnetischer Strahlung (,,KiSS" – Kindliche Leukämien und Expositionen in der Umgebung von hochfrequenten Sendestationen soll die Exposition durch leistungsstarke analoge Rundfunksender retrospektiv (Zeitraum 1982–2003 quantifiziert werden. Die zu betrachtenden Sendernetze für AM-Hörfunk, FM-Hörfunk und analoges Fernsehen unterscheiden sich nicht nur hinsichtlich der Modulationsart und der von den Einzelsendern abgestrahlten Leistung, sondern auch in der Netzkonfiguration und den je nach Wellenbereich verschiedenen Strahlungseigenschaften der Sendeantennen. Damit sind bei diesen drei Rundfunkdiensten sowohl die absolute Größe als auch die räumliche Struktur der hervorgerufenen Exposition verschieden. Es wird dargelegt, wie die für die Prognose der Rundfunkversorgung verwendeten Rechenverfahren zur Modellierung der Feldstärke für die Modellierung der Exposition herangezogen und durch Kontrollmessungen validiert werden und wie trotz Wahrung der Vertraulichkeit der dabei unabdingbar zu verwendenden Senderbetriebsdaten eine unerwünschte Beeinflussung der Studienergebnisse durch die Senderbetreiber ausgeschlossen wird.

  20. Development of a ssDNA aptamer for detection of residual benzylpenicillin.

    Science.gov (United States)

    Lee, A-Young; Ha, Na-Reum; Jung, In-Pil; Kim, Sang-Heon; Kim, A-Ru; Yoon, Moon-Young

    2017-08-15

    Antibiotics are useful for improving the living conditions of livestock. However, residual antibiotics induce several human diseases such as food-borne illness and infection of carbapenem-resistant Enterobacteriaceae (CRE). In this study, the identification of a benzylpenicillin-specific aptamer was selected by rGO-SELEX (reduced Graphene Oxide-Systematic Evolution of Ligands by EXponential enrichment). A random ssDNA library was incubated with rGO for adsorption and eluted with benzylpenicillin. As a result of the selection process, a DNA aptamer was found that specifically bound to benzylpenicillin with high binding affinity, K d  = 383.4 nM, and had a low limit of detection (LOD) of 9.2 nM. The characterization of the aptamer was performed through the fluorescence recovery signal from rGO surface. In addition, detection of benzylpenicillin was performed in pretreated milk samples, and its detection accuracy was shown to be 100± 10%. This represented that BBA1 was used for fluorescence aptasensor system in real sample. Furthermore, this benzylpenicillin binding aptamer showed high specificity against other antibiotics except for ampicillin. With these advantageous characteristics, we expect that this aptamer could be applied to an on-site detection system for residual benzylpenicillin. Copyright © 2017 Elsevier Inc. All rights reserved.

  1. STATUS BAKU MUTU AIR LAUT PERAIRAN TELUK AMBON LUAR UNTUK WISATA BAHARI KAPAL TENGGELAM SS AQUILA

    Directory of Open Access Journals (Sweden)

    Guntur Adhi Rahmawan

    2017-09-01

    Full Text Available Ambon Bay waters consist of two parts, Inner Ambon Bay and Outer Ambon Bay separated by a gap that is narrow and shallow. Ambon Bay has a lot of functionality and usability both in transportation, conservation, and tourism. The existence of one of the sites SS. Aquila sinking ship that sank since May 27, 1958, became one of the tourist attraction diving in Ambon Bay. Determination of water pollution index Ambon Bay becomes very important to do as support material and development of sea travel. Determining pollution index is done by direct measurement using the sea water quality parameters Water Quality Checker (DKK TOA WQC Type-24, as well as laboratory analysis to determine the chemical parameters of seawater (pH, TSS, salinity, turbidity, oil, grease. The results showed that the waters of the Bay of Ambon Affairs based on some parameters water quality standard for marine tourism is still included in accordance with the standard criteria by Keputusan Menteri Negara Lingkungan Hidup Nomor: 51 Tahun 2004 on Guidelines for Determination of Water Quality Status.

  2. Neutron Imaging of Laser Melted SS316 Test Objects with Spatially Resolved Small Angle Neutron Scattering

    Directory of Open Access Journals (Sweden)

    Adam J. Brooks

    2017-12-01

    Full Text Available A novel neutron far field interferometer is explored for sub-micron porosity detection in laser sintered stainless steel alloy 316 (SS316 test objects. The results shown are images and volumes of the first quantitative neutron dark-field tomography at various autocorrelation lengths, ξ . In this preliminary work, the beam defining slits were adjusted to an uncalibrated opening of 0.5 mm horizontal and 5 cm vertical; the images are blurred along the vertical direction. In spite of the blurred attenuation images, the dark-field images reveal structural information at the micron-scale. The topics explored include: the accessible size range of defects, potentially 338 nm to 4.5 μ m, that can be imaged with the small angle scattering images; the spatial resolution of the attenuation image; the maximum sample dimensions compatible with interferometry optics and neutron attenuation; the procedure for reduction of the raw interferogram images into attenuation, differential phase contrast, and small angle scattering (dark-field images; and the role of neutron far field interferometry in additive manufacturing to assess sub-micron porosity.

  3. PN Sequence Preestimator Scheme for DS-SS Signal Acquisition Using Block Sequence Estimation

    Directory of Open Access Journals (Sweden)

    Sang Kyu Park

    2005-03-01

    Full Text Available An m-sequence (PN sequence preestimator scheme for direct-sequence spread spectrum (DS-SS signal acquisition by using block sequence estimation (BSE is proposed and analyzed. The proposed scheme consists of an estimator and a verifier which work according to the PN sequence chip clock, and provides not only the enhanced chip estimates with a threshold decision logic and one-chip error correction among the first m received chips, but also the reliability check of the estimates with additional decision logic. The probabilities of the estimator and verifier operations are calculated. With these results, the detection, the false alarm, and the missing probabilities of the proposed scheme are derived. In addition, using a signal flow graph, the average acquisition time is calculated. The proposed scheme can be used as a preestimator and easily implemented by changing the internal signal path of a generally used digital matched filter (DMF correlator or any other correlator that has a lot of sampling data memories for sampled PN sequence. The numerical results show rapid acquisition performance in a relatively good CNR.

  4. Analysis of the Corrosion Behavior of an A-TIG Welded SS 409 Weld Fusion Zone

    Science.gov (United States)

    Vidyarthy, R. S.; Dwivedi, D. K.

    2017-11-01

    AISI 409 (SS 409) ferritic stainless steel is generally used as the thick gauge section in freight train wagons, in ocean containers, and in sugar refinery equipment. Activating the flux tungsten inert gas (A-TIG) welding process can reduce the welding cost during fabrication of thick sections. However, corrosion behavior of the A-TIG weld fusion zone is a prime concern for this type of steel. In the present work, the effect of the A-TIG welding process parameters on the corrosion behavior of a weld fusion zone made of 8-mm-thick AISI 409 ferritic stainless-steel plate has been analyzed. Potentiodynamic polarization tests were performed to evaluate the corrosion behavior. The maximum corrosion potential ( E corr) was shown by the weld made using a welding current of 215 A, a welding speed of 95 mm/min, and a flux coating density of 0.81 mg/cm2. The minimum E corr was observed in the weld made using a welding current of 190 A, a welding speed of 120 mm/min, and a flux coating density of 1.40 mg/cm2. The current study also presents the inclusive microstructure-corrosion property relationships using the collective techniques of scanning electron microscopy, energy-dispersive x-ray spectroscopy, and x-ray diffraction.

  5. Model Atmosphere Spectrum Fit to the Soft X-Ray Outburst Spectrum of SS Cyg

    Directory of Open Access Journals (Sweden)

    V. F. Suleimanov

    2015-02-01

    Full Text Available The X-ray spectrum of SS Cyg in outburst has a very soft component that can be interpreted as the fast-rotating optically thick boundary layer on the white dwarf surface. This component was carefully investigated by Mauche (2004 using the Chandra LETG spectrum of this object in outburst. The spectrum shows broad ( ≈5 °A spectral features that have been interpreted as a large number of absorption lines on a blackbody continuum with a temperature of ≈250 kK. Because the spectrum resembles the photospheric spectra of super-soft X-ray sources, we tried to fit it with high gravity hot LTE stellar model atmospheres with solar chemical composition, specially computed for this purpose. We obtained a reasonably good fit to the 60–125 °A spectrum with the following parameters: Teff = 190 kK, log g = 6.2, and NH = 8 · 1019 cm−2, although at shorter wavelengths the observed spectrum has a much higher flux. The reasons for this are discussed. The hypothesis of a fast rotating boundary layer is supported by the derived low surface gravity.

  6. Homozygous hemoglobin S (HbSS) presenting with bilateral facial nerve palsy: a case report.

    Science.gov (United States)

    Ogundunmade, Babatunde Gbolahan; Jasper, Unyime Sunday

    2014-10-16

    Bilateral facial nerve palsy is a relatively rare presentation and often points to a serious underlying medical condition. Several studies have reported presentation of bilateral facial nerve palsy in association with Lyme disease, Guillain-Barre syndrome, systemic lupus erythematosus, human immunodeficiency virus, sarcoidosis, diabetes and Hanson disease. While unilateral facial nerve palsy is sometimes associated with hemiplegia in sickle cell patients, no case of bilateral facial nerve palsy have been reported in the literature. A 29-year-old black African woman who is a known homozygous haemoglobin S (HbSS) presented with bilateral facial nerve palsy. She had the said condition 2 months post delivery of her first child and reported for physiotherapy 3 months post incidence. The pre-treatment House Brackmann Facial Grading Scale (HBFGS) Scores were 3 for right side and 4 for left side. This patient was not on any medication for the facial palsy. After 4 sessions of combination therapy consisting of faradism, facial exercises and massage there was remarkable improvement in the neurological status of the facial muscles. The post treatment House Brackmann Facial Grading Scale score was 2 bilaterally. Bilateral facial nerve palsy may be an initial presentation of sickle cell anemia patients in the absence of other overt clinical presentations. Therefore sickle cell anemia should be considered among others, in the differential diagnosis of bilateral facial nerve palsy. Furthermore, this case report has highlighted the important role of physiotherapy in the management of bilateral facial nerve palsy.

  7. The Blattodea s.s. (Insecta, Dictyoptera of the Guiana Shield

    Directory of Open Access Journals (Sweden)

    Dominic A. Evangelista

    2015-01-01

    Full Text Available Here we provide a checklist of cockroach species known from areas within the Guiana Shield based on literature records and new field collection. We give records of sixteen species collected in Guyana, eight of which are new records for Guyana and one of which is a new generic record for the entire Guiana Shield. We also provide a description for a geographically disparate species of Calhypnorna Stal, and the new species Xestoblatta berenbaumae. The complete checklist contains 234 species of Blattodea s.s. currently known in the shield. This checklist shows particularly low richness in Guianan Venezuela, Roraima and Amapa Brazil, but this is likely an artifact due to under–sampling. Indeed, based on previously published data and current fieldwork, we believe that most regions of the Guiana Shield are under–sampled for cockroaches. Despite this, French Guiana (151 spp. and Suriname (136 spp. rank as the second and sixth most species dense faunas of cockroaches in the neotropics.

  8. The Blattodea s.s. (Insecta, Dictyoptera) of the Guiana Shield

    Science.gov (United States)

    Evangelista, Dominic A.; Chan, Kimberly; Kaplan, Kayla L.; Wilson, Megan M.; Ware, Jessica L.

    2015-01-01

    Abstract Here we provide a checklist of cockroach species known from areas within the Guiana Shield based on literature records and new field collection. We give records of sixteen species collected in Guyana, eight of which are new records for Guyana and one of which is a new generic record for the entire Guiana Shield. We also provide a description for a geographically disparate species of Calhypnorna Stal, and the new species Xestoblatta berenbaumae. The complete checklist contains 234 species of Blattodea s.s. currently known in the shield. This checklist shows particularly low richness in Guianan Venezuela, Roraima and Amapa Brazil, but this is likely an artifact due to under–sampling. Indeed, based on previously published data and current fieldwork, we believe that most regions of the Guiana Shield are under–sampled for cockroaches. Despite this, French Guiana (151 spp.) and Suriname (136 spp.) rank as the second and sixth most species dense faunas of cockroaches in the neotropics. PMID:25684997

  9. El trabajo artesanal en Augusta Emerita durante los ss. I-IV d. C

    Directory of Open Access Journals (Sweden)

    Macarena BUSTAMANTE ÁLVAREZ

    2014-01-01

    Full Text Available Actualmente en la antigua capital lusitana es posible examinar, además de los restos arquitectónicos de sus edificios públicos y de una espléndida musivaria en ámbito doméstico, los indicios de otras producciones locales a las que hasta ahora no se les había concedido más que un interés de tipo secundario. En el presente trabajo damos a conocer una síntesis de las evidencias arqueológicas existentes del artesanado emeritense durante los ss. I-IV d. C. Este tema había sido revisado de manera aislada y esporádica en este solar. Aquí se valoran las estructuras, modos productivos y posibles evidencias de la mano de obra usada. Las constantes excavaciones que se han desarrollado en las últimas décadas han sacado a la luz 33 hornos cerámicos, 2 molinos alimentarios, 7 fullonicae, 1 officina infectae, 4 talleres de vidrio, 1 lanifricaria, 2 talleres de hueso, 2 pistrinae, 2 hornos metalúrgicos y 3 caleras que permiten el desarrollo de estudios de cierta envergadura. La industrial textil, la alfarera, alimentaria o metalúrgica, entre otras, son analizadas arqueológicamente. A partir de ello ha sido posible establecer un mapa de distribución de las distintas áreas manufactureras y de sus posibles zonas de influencia.

  10. Melanoma inhibitor of apoptosis protein (ML-IAP) specific cytotoxic T lymphocytes cross-react with an epitope from the auto-antigen SS56

    DEFF Research Database (Denmark)

    Baek Sørensen, Rikke; Faurschou, Mikkel; Troelsen, Lone

    2009-01-01

    A large proportion of melanoma patients host a spontaneous T-cell response specifically against ML-IAP-derived peptides. In this study, we describe that some ML-IAP-specific cytotoxic T cells isolated from melanoma patients cross react with an epitope from the auto-antigen SS56. SS56 is a recently...

  11. Roles of C-Terminal Region of Yeast and Human Rad52 in Rad51-Nucleoprotein Filament Formation and ssDNA Annealing.

    Directory of Open Access Journals (Sweden)

    Nilesh V Khade

    Full Text Available Yeast Rad52 (yRad52 has two important functions at homologous DNA recombination (HR; annealing complementary single-strand DNA (ssDNA molecules and recruiting Rad51 recombinase onto ssDNA (recombination mediator activity. Its human homolog (hRAD52 has a lesser role in HR, and apparently lacks mediator activity. Here we show that yRad52 can load human Rad51 (hRAD51 onto ssDNA complexed with yeast RPA in vitro. This is biochemically equivalent to mediator activity because it depends on the C-terminal Rad51-binding region of yRad52 and on functional Rad52-RPA interaction. It has been reported that the N-terminal two thirds of both yRad52 and hRAD52 is essential for binding to and annealing ssDNA. Although a second DNA binding region has been found in the C-terminal region of yRad52, its role in ssDNA annealing is not clear. In this paper, we also show that the C-terminal region of yRad52, but not of hRAD52, is involved in ssDNA annealing. This suggests that the second DNA binding site is required for the efficient ssDNA annealing by yRad52. We propose an updated model of Rad52-mediated ssDNA annealing.

  12. Adaptation and Psychometric Properties of the Self-Efficacy/Social Support for Activity for Persons with Intellectual Disability Scale (SE/SS-AID) in a Spanish Sample

    Science.gov (United States)

    Cuesta-Vargas, Antonio Ignacio; Paz-Lourido, Berta; Lee, Miyoung; Peterson-Besse, Jana J.

    2013-01-01

    Background: In this study we aimed to develop a Spanish version of the Self-Efficacy/Social Support Scales for Activity for persons with Intellectual Disability (SE/SS-AID). Method: A cross-sectional study was carried out in a sample of 117 individuals with intellectual disability (ID). The SE/SS-AID scales were translated into Spanish and their…

  13. The Rsm regulon of plant growth-promoting Pseudomonas fluorescens SS101: role of small RNAs in regulation of lipopeptide biosynthesis

    NARCIS (Netherlands)

    Song, C.; Voort, van der M.; Mortel, van de J.; Hassan, K.A.; Elbourne, L.D.H.; Paulsen, I.T.; Loper, J.E.; Raaijmakers, J.M.

    2015-01-01

    The rhizobacterium Pseudomonas fluorescens SS101 inhibits growth of oomycete and fungal pathogens, and induces resistance in plants against pathogens and insects. To unravel regulatory pathways of secondary metabolite production in SS101, we conducted a genome-wide search for sRNAs and performed

  14. AB006. Erectile dysfunction (ED) as a marker for cardiovascular diseases (CVD)

    Science.gov (United States)

    Torres, Luiz Otavio

    2015-01-01

    In 1973 V. Michal, a vascular surgeon said “Erectile dysfunction (ED) is related to diseases of the vascular bed”. And this makes sense since ED and cardiovascular diseases (CVD) share many risk factors like aging, obesity, inactivity, smoking, depression, dyslipidemia, hypertension, diabetes/insuline resistance. These conditions may lead to an oxidative stress which ultimately can promote vasoconstriction, thrombosis, atherosclerosis and finally ED and CVD. One of the most accepted Idea is that small vessels plug earlier, it means, small arteries when have for example 50% of obstruction will probably have a clinical manifestation before bigger arteries!

  15. [Efficacy of low-dose tadalafil on ED assessed by Self-Esteem and Relationship Questionnaire].

    Science.gov (United States)

    Li, Jing-Ping; Li, Fei; Guo, Wen-Bin; Zhou, Qi-Zhao; Liu, Cun-Dong; Mao, Xiang-Ming; Tan, Wan-Long; Zheng, Shao-Bin

    2010-12-01

    To explore the effects of low-dose oral tadalafil on self-esteem, confidence and sexual relationship in ED patients. We treated 17 ED patients with oral tadalafil at the low dose of 5 mg once daily for 12 weeks, and used the paired t test to compare their scores on The Self-Esteem and Relationship Questionnaire (SEAR) and IIEF-5 and the results of nocturnal penile tumescence (NPT) obtained by nocturnal electrobioimpedance volumetric assessment (NEVA) before and after the medication. The scores on SEAR and IIEF-5 were significantly increased (P self-esteem, confidence, sexual relationship satisfaction and NPT of ED patients.

  16. Optimization of the diffusion bonding parameters for SS316L/CuCrZr with and without Nickel interlayer

    Energy Technology Data Exchange (ETDEWEB)

    Singh, K.P., E-mail: kpsingh@ipr.res.in; Patel, Alpesh; Bhope, Kedar; Khirwadkar, S.S.; Mehta, Mayur

    2016-11-15

    Highlights: • Optimization of diffusion bonding parameters for dissimilar materials SS316L with CuCrZr is proposed with and without Nickel interlayer. • Ultrasonic testing on diffusion bonded samples provides the overall information/signatures about well-bonded and de-bonded samples. • Microstructural examination confirms the diffusion of Nickel into parent materials. • Mechanical characterization such as hardness measurement and shear measurement supports the results. - Abstract: Divertor & Firstwall module of ITER like tokamak has several joints of dissimilar materials such as Tungsten (W) to Copper (Cu), Cu to CuCrZr (heat sink material) and CuCrZr to Stainless Steel (SS) materials. These Plasma Facing Components (PFC) are made of multi-layered joints, which are to be exposed to the harsh environment of Plasma with the expected heat flux of 5–10 MW/m{sup 2}. The joining of SS316L material to CuCrZr material is proposed in this paper. As SS316L/CuCrZr being dissimilar materials, direct joining of these materials is a problem, which needs a suitable fabrication process. The joining of SS316L with heat sink material (CuCrZr) requires the good thermal transfer and sound in structural joint. Diffusion bonding technique has been adopted as a process for joining of these two dissimilar materials. The primary objective of the joining study is to obtain the best diffusion bonding parameters for ITER like tokamak application. Thermo-mechanical simulator machine (Gleeble 3800) is used to perform diffusion bonding experiments at different temperatures 650 °C, 850 °C, 900 °C, 950 °C & 1000 °C, different uniaxial pressure varying from 5 MPa to 15 MPa and with the holding time of 15 min & 30 min, using pure nickel interlayer. The diffusion bonded samples have undergone Non-destructive testing (NDT) particularly the ultrasonic examination using immersion probe technique, microstructural examination by the High Resolution (HR) electron microscopy (SEM), Energy

  17. The Type IX Secretion System (T9SS): Highlights and Recent Insights into Its Structure and Function

    Science.gov (United States)

    Lasica, Anna M.; Ksiazek, Miroslaw; Madej, Mariusz; Potempa, Jan

    2017-01-01

    Protein secretion systems are vital for prokaryotic life, as they enable bacteria to acquire nutrients, communicate with other species, defend against biological and chemical agents, and facilitate disease through the delivery of virulence factors. In this review, we will focus on the recently discovered type IX secretion system (T9SS), a complex translocon found only in some species of the Bacteroidetes phylum. T9SS plays two roles, depending on the lifestyle of the bacteria. It provides either a means of movement (called gliding motility) for peace-loving environmental bacteria or a weapon for pathogens. The best-studied members of these two groups are Flavobacterium johnsoniae, a commensal microorganism often found in water and soil, and Porphyromonas gingivalis, a human oral pathogen that is a major causative agent of periodontitis. In P. gingivalis and some other periodontopathogens, T9SS translocates proteins, especially virulence factors, across the outer membrane (OM). Proteins destined for secretion bear a conserved C-terminal domain (CTD) that directs the cargo to the OM translocon. At least 18 proteins are involved in this still enigmatic process, with some engaged in the post-translational modification of T9SS cargo proteins. Upon translocation across the OM, the CTD is removed by a protease with sortase-like activity and an anionic LPS is attached to the newly formed C-terminus. As a result, a cargo protein could be secreted into the extracellular milieu or covalently attached to the bacterial surface. T9SS is regulated by a two-component system; however, the precise environmental signal that triggers it has not been identified. Exploring unknown systems contributing to bacterial virulence is exciting, as it may eventually lead to new therapeutic strategies. During the past decade, the major components of T9SS were identified, as well as hints suggesting the possible mechanism of action. In addition, the list of characterized cargo proteins is

  18. Regulation of hypothalamic expression of KiSS-1 and GPR54 genes by metabolic factors: analyses using mouse models and a cell line.

    Science.gov (United States)

    Luque, Raul M; Kineman, Rhonda D; Tena-Sempere, Manuel

    2007-10-01

    It is well established that reproductive function is metabolically gated. However, the mechanisms whereby energy stores and metabolic cues influence fertility are yet to be completely deciphered. Recently, the hypothalamic KiSS-1/GPR54 system has emerged as a fundamental regulator of the gonadotropic axis, which conveys the modulatory actions of sex steroids to GnRH neurons. Evidence is also mounting that KiSS-1 neurons may also represent the link between systemic metabolic signals and central control of reproduction. To further explore this possibility, we examined the impact of changes in energy status and key metabolic regulators on the hypothalamic expression of KiSS-1 and GPR54 genes, using different mouse models and the hypothalamic cell line N6. Time-course analysis of the effects of short-term fasting revealed a rapid (12- and 24-h) decline in KiSS-1 and GPR54 mRNA levels, which preceded that of GnRH (48 h). In contrast, diet-induced obesity or obesity associated with leptin deficiency (ob/ob vs. wild-type mice) failed to induce overt changes in hypothalamic expression of KiSS-1 and GPR54 genes. However, leptin infusion of ob/ob mice evoked a significant increase in KiSS-1 and GPR54 mRNA levels compared with pair-fed controls. Moreover, leptin, but not insulin or IGF-I, stimulated KiSS-1 mRNA expression in the mouse hypothalamic cell line N6. In addition, neuropeptide Y (NPY) null mice showed decreased KiSS-1 mRNA levels at the hypothalamus, whereas exposure to NPY increased expression of KiSS-1 in hypothalamic N6 cells. In sum, our present data further characterize the functional relevance and putative key mediators (such as leptin and NPY) of the metabolic regulation of the hypothalamic KiSS-1 system in the mouse.

  19. Involvement of NpdA, a Putative 2-Nitropropane Dioxygenase, in the T3SS Expression and Full Virulence in Ralstonia solanacearum OE1-1

    Directory of Open Access Journals (Sweden)

    Weiqi Zhang

    2017-10-01

    Full Text Available Previously, we isolated several genes that potentially affected the expression of type III secretion system (T3SS in Ralstonia solanacearum OE1-1. Here, we focused on the rsp0316, which encodes a putative 2-nitropropane dioxygenase (hereafter designated NpdA. The deletion of npdA substantially reduced the T3SS expression and virulence in OE1-1, and the complementation with functional NpdA could completely restore its reduced T3SS expression and virulence to that of wild type. The NpdA was highly conserved among diverse R. solanacearum species and the NpdA-dependent expression of T3SS was not specific to OE1-1 strain, but not the virulence. The NpdA was important for the T3SS expression in planta, while it was not required for the bacterial growth in planta. Moreover, the NpdA was not required for the elicitation of hypersensitive response (HR of R. solanacearum strains in tobacco leaves. The T3SS in R. solanacearum is directly controlled by the AraC-type transcriptional regulator HrpB and regulated by a complex regulation network. The NpdA affected the T3SS expression mediated with HrpB but through some novel pathway. All these results from genetic studies demonstrate that NpdA is a novel factor for the T3SS expression in diverse R. solanacearum species in medium, but specifically for the T3SS expression in strain OE1-1 in planta. And the NpdA-dependent expression of T3SS in planta plays an important role in pathogenicity of R. solanacearum OE1-1 in host plants.

  20. Involvement of NpdA, a Putative 2-Nitropropane Dioxygenase, in the T3SS Expression and Full Virulence in Ralstonia solanacearum OE1-1.

    Science.gov (United States)

    Zhang, Weiqi; Li, Jing; Tang, Yu; Chen, Kai; Shi, Xiaojun; Ohnishi, Kouhei; Zhang, Yong

    2017-01-01

    Previously, we isolated several genes that potentially affected the expression of type III secretion system (T3SS) in Ralstonia solanacearum OE1-1. Here, we focused on the rsp0316, which encodes a putative 2-nitropropane dioxygenase (hereafter designated NpdA). The deletion of npdA substantially reduced the T3SS expression and virulence in OE1-1, and the complementation with functional NpdA could completely restore its reduced T3SS expression and virulence to that of wild type. The NpdA was highly conserved among diverse R. solanacearum species and the NpdA-dependent expression of T3SS was not specific to OE1-1 strain, but not the virulence. The NpdA was important for the T3SS expression in planta, while it was not required for the bacterial growth in planta. Moreover, the NpdA was not required for the elicitation of hypersensitive response (HR) of R. solanacearum strains in tobacco leaves. The T3SS in R. solanacearum is directly controlled by the AraC-type transcriptional regulator HrpB and regulated by a complex regulation network. The NpdA affected the T3SS expression mediated with HrpB but through some novel pathway. All these results from genetic studies demonstrate that NpdA is a novel factor for the T3SS expression in diverse R. solanacearum species in medium, but specifically for the T3SS expression in strain OE1-1 in planta. And the NpdA-dependent expression of T3SS in planta plays an important role in pathogenicity of R. solanacearum OE1-1 in host plants.

  1. Effects of a new antiarrhythmic drug SS-68 on electrical activity in working atrial and ventricular myocardium of mouse and their ionic mechanisms

    Directory of Open Access Journals (Sweden)

    Saida K. Bogus

    2015-08-01

    Full Text Available SS-68 is a derivative of indole, which demonstrated strong antiarrhythmic effects not associated with significant QT prolongation in dog models of atrial fibrillation. Therefore, SS-68 was proposed as a new antiarrhythmic drug and the present study is the first describing its effects on action potentials (APs configuration and elucidating the ionic mechanisms of these effects. Sharp microelectrodes were used to record APs in isolated preparations of mouse atrial and ventricular myocardium. In both types of myocardium 10−6 M SS-68 produced reduction of AP duration, 3 × 10−6 M failed to alter AP waveform and 10−5 – 3 × 10−5 M prolonged APs. Sensitivity of main ionic currents to SS-68 was determined using whole-cell patch clamp. Transient potassium current Ito was slightly inhibited by SS-68 with IC50 = 1.43 × 10−4 M. IKur was more sensitive with IC50 = 1.84 × 10−5 M. Background inward rectifier showed very low sensitivity to SS-68 – only 10−4 M SS-68 caused significant reduction of IK1. ICaL was significantly inhibited by 10−6M – 3 × 10−5 M SS-68. The IC50 value for the ICaL was 1.84 × 10−6 M. Thus, main ionic currents of mouse cardiomyocytes are inhibited by SS-68 in the following order of potency: ICaL > IKur > Ito > IK1. While lower concentration of SS-68 shorten APs via suppression of ICaL, higher concentrations inhibit K+-currents leading to APs prolongation.

  2. High-Resolution X-Ray Spectra of the Symbiotic Star SS73 17

    Science.gov (United States)

    Eze, R. N. C.; Luna, G. J. M.; Smith, R. K.

    2010-01-01

    SS73 17 was an innocuous Mira-type symbiotic star until the International Gamma-Ray Astrophysics Laboratory and Swift discovered its bright hard X-ray emission, adding it to the small class of "hard X-ray emitting symbiotics." Suzaku observations in 2006 then showed it emits three bright iron lines as well, with little to no emission in the 0.3-2.0 keV bandpass. We present here follow-up observations with the Chandra High Energy Transmission Grating and Suzaku that confirm the earlier detection of strong emission lines of Fe K(alpha) fluorescence, Fe XXV and Fe XXVI but also show significantly more soft X-ray emission. The high-resolution spectrum also shows emission lines of other highly ionized ions as Si xiv and possibly S XVI. In addition, a re-analysis of the 2006 Suzaku data using the latest calibration shows that the hard (15-50 keV) X-ray emission is brighter than previously thought and remains constant in both the 2006 and 2008 data. The G ratio calculated from the Fe xxv lines shows that these lines are thermal, not photoionized, in origin.With the exception of the hard X-ray emission, the spectra from both epochs can be fit using thermal radiation assuming a differential emission measure based on a cooling-flow model combined with a full and partial absorber. We show that acceptable fits can be obtained for all the data in the 1-10 keV band varying only the partial absorber. Based on the temperature and accretion rate, the thermal emission appears to be arising from the boundary layer between the accreting white dwarf and the accretion disk.

  3. A Comparative Analysis of the Structural Architecture of ssDNA Viruses

    Directory of Open Access Journals (Sweden)

    Antonette Bennett

    2008-01-01

    Full Text Available Virus assembly, utilizing a limited number of viral coat protein (CP or VP building blocks, is an excellent example of a directed macromolecular interaction occurring in nature. Two basic principles govern the assembly of spherical (icosahedral viruses: (i Genetic Economy – the encapsidated genome encodes a single or few CPs that assemble a protective shell (the viral capsid; and (ii specificity – the CPs have to fold to recognize each other and form exact CP–CP interfacial interactions during the assembly pathway. Using a variety of biophysical techniques, including X-ray crystallography and cryo-electron microscopy, combined with homology model building, biochemistry and molecular biology, the nature of the interactions between protein–protein subunits and protein–nucleic acid that facilitate viral capsid assembly have been studied. This review discusses both the similarities and differences that have been elucidated for ssDNA Microviridae, Geminiviridae, and Parvoviridae virus families. The Microviridae represent a family of bacteriophages that utilize several CPs and scaffold proteins to assemble a T = 1 icosahedral capsid, the Geminiviridae plant viruses assemble a unique twinned quasi-isometric (geminate pseudo T = 1 virion assembled from a single CP and the Parvoviridae represent animal viruses whose T = 1 capsids are formed from the common overlapping region of two to four VPs that have unique N-terminal extensions. A survey of the three-dimensional (3D data available for these viruses shows that they utilize structural commonalities, facilitated by disparate CP/VP amino acid sequences for the successful assembly of mature infectious virions.

  4. Physical properties and microstructure study of 316L SS fabricated by metal injection moulding process

    Science.gov (United States)

    Dandang, Nur Aidah Nabihah; Harun, Wan Sharuzi Wan; Khalil, Nur Zalikha; Ismail, Muhammad Hussain; Ibrahim, Rosdi

    2017-12-01

    Metal injection moulding (MIM) has been practised to process alloy powders to become components with significant physical and mechanical properties. Dissimilar than other methods, MIM focuses on the production of high volume, a small, and complex shape of products. The performance of the compacts depends on the suitable sintering parameters that governs their strengths in the final phase which determines the excellent properties of the sintered compacts. Three different sintering temperatures were utilised; 1100, 1200, and 1300 °C with two different soaking times; 1 and 3 hours at 10 °C/min heating rate to study their effect on the physical properties and microstructure analysis of 316L SS alloy compacts. The shrinkage measurement, surface roughness, and density measurement had been conducted for physical properties study. Different sintering temperatures give an effect to the physical properties of the sintered compacts. The shrinkage measurement at 1300 °C and 3-hour sintering condition demonstrated the highest percentage reading which was 10.1 % compared to the lowest percentage reading of 6.4 % at 1100 °C and 1-hour sintering conditions. Whereas, the minimum percentage of density measurement can be found at sintering conditions of 1100 °C and 1-hour which is 83.9 % and the highest percentage is at 1300 °C and 3-hour sintering condition which is about 89.51 %. Therefore, it has been determined that there could be a significant relationship between sintering temperature and physical properties in which it can be found from the porosity of the compact based on the microstructure studies.

  5. Chandra Low Energy Transmission Grating Spectrum of SS Cygni in Outburst

    Energy Technology Data Exchange (ETDEWEB)

    Mauche, C W

    2004-02-20

    We have fitted the Chandra Low Energy Transmission Grating spectrum of SS Cygni in outburst with a single temperature blackbody suffering the photoelectric opacity of a neutral column density and the scattering opacity of an outflowing wind. We find that this simple model is capable of reproducing the essential features of the observed spectrum with the blackbody temperature T{sub bl} {approx} 250{+-}50 kK, hydrogen column density N{sub H} {approx} 5.0{sup +2.9}{sub -1.5}x10{sup 19} cm{sup -2}, fractional emitting area f {approx} 5.6{sup +60}{sub -4.5} x10{sup -3}, boundary layer luminosity Lbl {approx} 5{sup +18}{sub -3} x10{sup 33} erg s{sup -1}, wind velocity v {approx} 2500 km s{sup -1}, wind mass-loss rate w {approx} 1.1x10{sup 16} g s{sup -1}, and arbitrary values of the wind ionization fractions of 20 ions of O, Ne,Mg, Si, S, and Fe. Given that in outburst the accretion disk luminosity L{sub disk} {approx}1x10{sup 35} erg s{sup -1}, L{sub bl}/L{sub disk} {approx} 0.05{sup +0.18}{sub -0.03}, which can be explained if the white dwarf (or an equatorial belt thereon) is rotating with an angular velocity {Omega}{sub wd} {approx} 0.7{sup +0.1}{sub -0.2}Hz, hence V{sub rot}sini {approx} 2300 km s{sup -1}.

  6. SS-A/Ro52 promotes apoptosis by regulating Bcl-2 production

    Energy Technology Data Exchange (ETDEWEB)

    Jauharoh, Siti Nur Aisyah [Department of Clinical Pathology and Immunology, Kobe University Graduate School of Medicine, Hyogo 650-0017 (Japan); Faculty of Medicine and Health Science, Syarif Hidayatullah State Islamic University, Jakarta 15412 (Indonesia); Saegusa, Jun; Sugimoto, Takeshi [Department of Clinical Pathology and Immunology, Kobe University Graduate School of Medicine, Hyogo 650-0017 (Japan); Ardianto, Bambang [Department of Clinical Pathology and Immunology, Kobe University Graduate School of Medicine, Hyogo 650-0017 (Japan); Department of Child Health, Faculty of Medicine, Gadjah Mada University, Yogyakarta 55282 (Indonesia); Kasagi, Shimpei; Sugiyama, Daisuke; Kurimoto, Chiyo [Department of Clinical Pathology and Immunology, Kobe University Graduate School of Medicine, Hyogo 650-0017 (Japan); Tokuno, Osamu; Nakamachi, Yuji [Department of Laboratory Medicine, Kobe University Hospital, Hyogo 650-0017 (Japan); Kumagai, Shunichi [Department of Clinical Pathology and Immunology, Kobe University Graduate School of Medicine, Hyogo 650-0017 (Japan); Kawano, Seiji, E-mail: sjkawano@med.kobe-u.ac.jp [Department of Clinical Pathology and Immunology, Kobe University Graduate School of Medicine, Hyogo 650-0017 (Japan); Department of Laboratory Medicine, Kobe University Hospital, Hyogo 650-0017 (Japan)

    2012-01-06

    Highlights: Black-Right-Pointing-Pointer Ro52{sup low} HeLa cells are resistant to apoptosis upon various stimulations. Black-Right-Pointing-Pointer Ro52 is upregulated by IFN-{alpha}, etoposide, or IFN-{gamma} and anti-Fas Ab. Black-Right-Pointing-Pointer Ro52-mediated apoptosis is independent of p53. Black-Right-Pointing-Pointer Ro52 selectively regulates Bcl-2 expression. -- Abstract: SS-A/Ro52 (Ro52), an autoantigen in systemic autoimmune diseases such as systemic lupus erythematosus and Sjoegren's syndrome, has E3 ligase activity to ubiquitinate proteins that protect against viral infection. To investigate Ro52's role during stress, we transiently knocked it down in HeLa cells by siRo52 transfection. We found that Ro52{sup low} HeLa cells were significantly more resistant to apoptosis than wild-type HeLa cells when stimulated by H{sub 2}O{sub 2}- or diamide-induced oxidative stress, IFN-{alpha}, IFN-{gamma} and anti-Fas antibody, etoposide, or {gamma}-irradiation. Furthermore, Ro52-mediated apoptosis was not influenced by p53 protein level in HeLa cells. Depleting Ro52 in HeLa cells caused Bcl-2, but not other Bcl-2 family molecules, to be upregulated. Taken together, our data showed that Ro52 is a universal proapoptotic molecule, and that its proapoptotic effect does not depend on p53, but is exerted through negative regulation of the anti-apoptotic protein Bcl-2. These findings shed light on a new physiological role for Ro52 that is important to intracellular immunity.

  7. GERMINATION TEST IN SEEDS OF MELOTHRIA CAMPESTRIS (NAUDIN H. SCHAEF. & S.S. RENNER

    Directory of Open Access Journals (Sweden)

    ANA CARINA DA SILVA CÂNDIDO

    2016-01-01

    Full Text Available The Cerrado occupies 25% of the Brazilian territory and is the second largest biome in South America, second only to the Amazon Rainforest. Hence, studies on the native species of the Cerrado are important for implementing conservation strategies. In this context, knowledge of the requirements and conditions for germination of seeds of these native species is essential. The Cerrado watermelon ( Melothria campestris (Naudin H. Schaef. & S.S. Renner is a plant that is not cultivated, but has been used in revegetation projects in the countryside of the Cerrado because its fruits are fed upon by fauna in times of food shortage. The purpose of this study was to determine the most appropriate methodology for the germination of the Cerrado watermelon, by identifying the best - suited substrate, the optimal temperature, and the corresponding time taken for germination . The treatments consisted of four substrates: in a paper roll, on paper, in sand, and on sand; and four temperatures: 20, 25, and 30 °C (maintained constant, and a fourth temperature treatment that alternated 20 - 30 °C. The effect of the substrates and temperatures on seed performance was evaluated using the germination test, speed index, and average time for germination. The experimental design was fully randomized, with treatments distributed in a 4 × 4 (substrates × temperatures factorial scheme, with four replications. Our results show that the temperature of 20 °C had a negative influence on germination in all substrates. Further, we found that the germination of the Cerrado watermelon seeds was best in a paper roll or on sand substrate, maintained at a constant temperature of 30 °C, or when temperature was alternated 20 - 30 °C, and the time required for germination stabilization was 30 days.

  8. Review: Funston, John (ed. (2009, Divided Over Thaksin: Thailand’s Coup and Problematic Transition

    Directory of Open Access Journals (Sweden)

    Paul Chambers

    2010-04-01

    Full Text Available Review of the edited volume: Funston, John (ed. (2009, Divided Over Thaksin: Thailand’s Coup and Problematic Transition, Chiangmai: Silkworm Books, Singapore: ISEAS. ISBN 978-981-230-961-7, 203 pages.

  9. Patient-Centered Care Transition for Patients Admitted through the ED: Improving Patient and Employee Experience

    National Research Council Canada - National Science Library

    Algauer, Andrea; Rivera, Stephanie; Faurote, Robert

    2015-01-01

    With increasing wait times in emergency departments (ED) across America, there is a need to streamline the inpatient admission process in order to decrease wait times and more important, to increase patient and employee satisfaction...

  10. Hispaania võib oma väed Iraagist ära tuua / Erkki Bahovski

    Index Scriptorium Estoniae

    Bahovski, Erkki, 1970-

    2004-01-01

    Hispaania parlamendivalimised võitnud sotsialistliku partei liider, arvatav uus peaminister Jose Luis Rodriguez Zapatero lubas Hispaania väed Iraagist ära tuua. Diagrammid: Hispaania valimistulemused

  11. Jean Libis, Fabio Ferreira, Catherine Gublin, Sarah Mezaguer (eds., Les Lectures de Gaston Bachelard. Index bibliographique

    Directory of Open Access Journals (Sweden)

    Aurosa Alison

    2012-10-01

    Full Text Available Jean Libis, Fabio Ferreira, Catherine Gublin, Sarah Mezaguer (eds., Les Lectures de Gaston Bachelard. Index bibliographique (Paris, Presse Universitaire de Franche-Comté, 2011 ISBN: 978-2-84867-392 di     Aurosa Alison

  12. G. L. Castelli, principe di Torremuzza, numismatico ed antichista ad Halaesa Archonidea

    National Research Council Canada - National Science Library

    Antonino Crisà

    2009-01-01

    ... dell’antiquaria siciliana del XVIII secolo. Questo contributo esamina l’attività di ricerca di Castelli, interessato alla monetazione ed alle evidenze archeologiche di Halaesa Archonidea, oggi identificata nei pressi di Tusa (ME). È proposta...

  13. In a dynamic health care environment, take steps to fully leverage case managers in the ED.

    Science.gov (United States)

    2013-09-01

    In an effort to create more capacity while also improving the patient experience, the University of Virginia Medical Center (UVAMC) in Charlottesville, VA, has taken steps to better leverage case managers in the ED. Under the new approach, case managers work alongside clinicians on the floor. They step in when added resources are needed or services need to be arranged before discharge. Administrators say the new ED case manager role has helped UVAMC reduce medically unnecessary length-of-stay by 34 minutes, enabling the hospital to see up to 4,000 additional patients per year. The ED-based case managers learn of patients who may benefit from their involvement by regularly participating in flow rounds with clinicians on the floor. Administrators are eyeing future improvements from the approach, including a concentrated effort to reduce visits to the ED by frequent utilizers.

  14. Drying Time and Quality of EDS-Treated Compared to Untreated Beech Wood (Fagus japonica

    Directory of Open Access Journals (Sweden)

    Mihaela CAMPEAN

    2017-09-01

    Full Text Available The paper presents the results of an experimental study performed with Japanese beech timber heattreated by the EDS method (Japanese patent and then dried in order to evaluate the effects of this treatment upon the drying rate and the drying uniformity. The obtained results demonstrate benefitting effects of the EDS treatment both upon the drying time and the drying quality. The drying rate of EDS-treated beech wood is by 29% higher in the case of wood without red heart and by 11% higher in the case of wood with red heart. As far as the drying uniformity is concerned, the minimum moisture content gradient across the 50mm thickness of the timber boards was recorded for the EDS-treated beech wood with red heart (∆MC=1.66, by 21.7% lower than in the case of untreated beech wood with red heart.

  15. 2003 annual results of EdF group; Resultats annuels 2003 du groupe EDF

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-03-01

    The Electricite de France (EdF) group Board of Directors, meeting on March 11, 2004, under the Chairmanship of Francois Roussely, reviewed the audited consolidated financial statements for the year ended 12/31/2003. This document presents the consolidated results of EdF group for 2003: consolidated financial statements, highlights of the year, focus on 2003 events, commercial results, EDF in Europe and worldwide, EDF France highlights, key figures. (J.S.)

  16. Bedside point of care toxicology screens in the ED: Utility and pitfalls

    OpenAIRE

    Bhalla, Ashish

    2014-01-01

    Exposure to drugs and toxins is a major cause for patients’ visits to the emergency department (ED). For most drugs-of-abuse intoxication, ED physicians are skeptical to rely on results of urine drug testing for emergent management decisions. This is partially because immunoassays, although rapid, have limitations in sensitivity and specificity and chromatographic assays, which are more definitive, are more labor intensive. Testing for toxic alcohols is needed, but rapid commercial assays are...

  17. Test Review: Gilliam, J. E. (2015), "Attention-Deficit/Hyperactivity Disorder Test" (2nd Ed) [Assessment Instrument]. Austin, TX: Pro-Ed.

    Science.gov (United States)

    Perdue, Elizabeth A.

    2016-01-01

    The "Attention-Deficit/Hyperactivity Disorder Test-Second Edition" (ADHDT-2) is published through Pro-Ed in Austin, Texas. It was formally published in 2014, following critical revisions of the ADHDT, the reportedly popular initial version of this test that was published in 1995. The ADHDT-2 purports to act as a screener for individuals…

  18. EdF-Gaz de France. No merger without dismembering; EdF-Gaz de France. Pas de fusion sans depecage

    Energy Technology Data Exchange (ETDEWEB)

    Lepetit, V

    2007-03-15

    Gathering together the two historical French energy monopolies, Electricite de France (EdF) and Gaz de France (GdF), and warranting low electricity and gas prices is still an attractive idea for some political and syndicate representatives. However, such a merger would create a dominating position which is forbidden with respect to Brussels criteria. (J.S.)

  19. Expression, purification and preliminary crystallographic analysis of the T6SS effector protein Tse3 from Pseudomonas aeruginosa.

    Science.gov (United States)

    Lu, Defen; Shang, Guijun; Yu, Qian; Zhang, Heqiao; Zhao, Yanyu; Cang, Huaixing; Gu, Lichuan; Xu, Sujuan; Huang, Yan

    2013-05-01

    Pseudomonas aeruginosa uses the type VI secretion system (T6SS) to inject effector proteins into rival cells in niche competition. Tse3, one of the effectors of T6SS, is delivered into the periplasm of recipient cells. Tse3 functions as a muramidase that degrades the β-1,4-linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) in peptidoglycan, thus leading to lysis of the recipient cells and providing a competitive advantage to the donor cells. Here, the preliminary crystallographic study of Tse3 is reported. A crystal of Tse3 diffracted to 1.5 Å resolution. It belonged to space group C121, with unit-cell parameters a = 166.99, b = 70.13, c = 41.94 Å, α = 90.00, β = 90.52, γ  = 90.00° and one molecule per asymmetric unit.

  20. Musiikin käyttö pitkäaikaissairaan ikäihmisen hoitotyössä

    OpenAIRE

    Kolmonen, Hanna; Huhtala, Marja

    2012-01-01

    Opinnäytetyömme tavoitteena oli saada tietoa musiikin käytöstä ja merkityksestä pitkäaikaissairaiden ikäihmisten hoitotyössä. Tutkimuksemme tarkoituksena oli kuvata musiikin käyttöä ja musiikin merkitystä hoitajien näkökulmasta Rovaniemen terveyskeskussairaalan pitkäaikaissairaiden vuodeosastolla P5. Lisäksi tarkoituksenamme oli kuvata hoitajien ehdotuksia musiikin käytön kehittämiseen ja hyödyntämiseen pitkäaikaissairaiden ikäihmisten hoitotyössä. Toteutimme opinnäytetyömme laadullisell...

  1. Leanin 5S toimistoympäristössä : Kehittämistyön tulokset

    OpenAIRE

    Korhonen, Tommi

    2014-01-01

    Tämän opinnäytetyön tavoitteena oli raportoida 5S-toimintamallin käyttöönotto Aditro Oy:n Kajaanin toimistolla. Opinnäytetyössä kuvataan 5S-toimintamallin käyttöönoton vaiheet ja niiden vaikutukset toimistoympäristössä. Lisäksi tarkastellaan miten Lean–ajattelulla ja 5S-toimintamallilla voidaan tehostaa toimistoympäristön työprosesseja. Lopuksi arvioidaan 5S-toimintamallista saatuja hyötyjä sekä käyttöönottoon liittyviä ongelmia. Teoreettisena taustana oli Lean-filosofian mukainen kirjal...

  2. A Study on Thermal Desorption of Deuterium in D-loaded SS316LN for ITER Tritium Removal System

    Energy Technology Data Exchange (ETDEWEB)

    Park, Myungchul; Kim, Heemoon; Ahn, Sangbok [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Kim, Jaeyong; Lee, Sanghwa; LanAhn, Nguyen Thi [Hanyang University, Seoul (Korea, Republic of)

    2016-10-15

    Because Type B radwaste includes tritium on its inside, especially at vicinity of surface, tritium removal from the radwaste is a matter of concern in terms of the radwaste processes. Tritium behavior in materials is related with temperature. Considering a diffusion process, it is expected that tritium removal efficiency is enhanced with increasing baking temperature. However, there is a limitation about temperature due to facility capacity and economic aspect. Therefore, it is necessary to investigate the effect of temperature on the desorption behavior of Tritium in ITER materials. TDS analysis was performed in SS316LN loaded at 120, 240 and 350 °C. D2 concentration and the desorption peak temperature increased with increasing loading temperature. Using peak shift method with three ramp rates of 0.166, 0.332, and 0.5 °C/sec, trap activation energy of D in SS316LN loaded at 350 °C was 56 kJ/mol.

  3. First report of Metarhizium anisopliae IP 46 pathogenicity in adult Anopheles gambiae s.s. and An. arabiensis (Diptera; Culicidae

    Directory of Open Access Journals (Sweden)

    Lyimo Issa N

    2009-12-01

    Full Text Available Abstract The entomopathogenic fungus Metarhizium anisopliae isolate IP 46, originating from a soil sample collected in 2001 in the Cerrado of Central Brazil, was tested for its ability to reduce the survival of adult male and female Anopheles gambiae s.s. and An. arabiensis mosquitoes. A 6-h exposure to the fungus coated on test paper at a concentration of 3.3 × 106 conidia cm-2 reduced the daily survival of both mosquito species (HR = 3.14, p An. gambiae s.s relative to An. arabiensis (HR = 1.38, p 95% of mosquito cadavers in the treatment groups. The results indicate that M. anisopliae IP 46 has the potential to be a bio-control agent for African malaria vector species, and is a suitable candidate for further research and development.

  4. Pulsed laser deposited MnCo2O4 protective layer on SS430 for solid oxide fuel cell application

    Science.gov (United States)

    Gaur, Anshu; Mohiddon, Md. Ahamad; Prasad, Muvva D.

    2016-05-01

    The growth and oxidation study of pulsed laser deposited MnCo2O4 protective layer on SS430 substrate for solid oxide fuel cell application is demonstrated. MnCo2O4 has been achieved in three different ways including, deposition at higher substrate temperature (700°C), and deposition at room temperature on pre-oxidized and untreated SS430 substrate followed by annealing at 700°C for 2 hrs. X-ray diffraction and Raman spectroscopy has been applied to demonstrate the kind of phases developed in each case. These three samples were subjected to heat treatment at 750°C for 5 hr. The extent of undesired Fe2O3 phase formation in the post deposition heat treated samples is discussed based on Raman spectroscopic results.

  5. Impact of revised triage to improve throughput in an ED with limited traditional fast track population.

    Science.gov (United States)

    Gardner, Ryan M; Friedman, Nathan A; Carlson, Michael; Bradham, Tamala S; Barrett, Tyler W

    2018-01-01

    Emergency department (ED) crowding is associated with patient safety concerns, increased patients left without being seen (LWBS), low patient satisfaction, and lost ED revenue. The objective was to measure the impact of a revised triage process on ED throughput. This study took place at an urban, university-affiliated, adult ED with an annual census of 70,000 and admission rate of 34%. The revised triage approach included: identifying eligible patients at triage based on complaint, comorbidities, and illness acuity; and reallocating a nurse practitioner (NP) into our triage area. We trialed the intervention from 1100-2300 on weekdays from January 13-26, 2016. Adult patients who were not likely to require intensive evaluations were eligible. Primary outcomes were throughput measures including: time to provider, ED length of stay (LOS), and LWBS. Pre- and post-intervention metrics were compared using the Mann-Whitney U test, given the non-normal distribution of the metrics. The NP evaluated 120 patients of which 101 (84%) were discharged, 3 (2.5%) admitted, and 16 (13%) required more intense evaluation. Time to provider decreased from a median (IQR) of 42 (16, 114) to 27 (12.4, 81.5) minutes (ptriage intervention was associated with improvements in several ED throughput metrics and a reduction in LWBS. Copyright © 2017 Elsevier Inc. All rights reserved.

  6. Registered nurse scope of practice and ED complaint-specific protocols.

    Science.gov (United States)

    Castner, Jessica; Grinslade, Susan; Guay, Jennifer; Hettinger, A Zach; Seo, Jin Young; Boris, Lenore

    2013-09-01

    The use of complaint-specific protocols (CSPs) by emergency registered nurses (RNs) can improve ED efficiency. However, RN practice is influenced by regulatory environments that may facilitate or inhibit the use of protocols. The purpose of this policy analysis was to explore the language of state boards of nursing scope-of-practice documents related to the use of RN-initiated CSPs in the ED setting. A qualitative descriptive design was used to investigate how the RN's scope of practice relates to the use of CSPs in ED settings across states. Data were collected from state boards of nursing Web site documents. Three major themes emerged: cautiously within scope, intentionally vague/silent, and outside scope. Seven states (Connecticut, Illinois, Kansas, Louisiana, New Jersey, New York, and Oklahoma) were identified where RN-initiated CSPs appear to be currently outside the RN's scope of practice. Therefore 18% of the US population, or 55,973,900 people, resides in states where CSPs are not allowed. State-to-state inconsistencies in the RN's scope of practice may interfere with the implementation of practices that enhance ED efficiency. RNs in all states must ensure that they have the requisite knowledge, skill, and documented competency to implement CSPs, if supported by their employing facility. Efforts to standardize ED RN education and policy are warranted. Continued research is needed assess the impact of RN-initiated CSPs on the efficiency of ED care. Copyright © 2013 Emergency Nurses Association. Published by Mosby, Inc. All rights reserved.

  7. Chemical analysis and biological testing of materials from the EDS coal liquefaction process: a status report

    Energy Technology Data Exchange (ETDEWEB)

    Later, D.W.; Pelroy, R.A.; Wilson, B.W.

    1984-05-01

    Representative process materials were obtained from the EDS pilot plant for chemical and biological analyses. These materials were characterized for biological activity and chemical composition using a microbial mutagenicity assay and chromatographic and mass spectrometric analytical techniques. The two highest boiling distillation cuts, as well as process solvent (PS) obtained from the bottoms recycle mode operation, were tested for initiation of mouse skin tumorigenicity. All three materials were active; the crude 800/sup 0 +/F cut was substantially more potent than the crude bottoms recycle PS or 750 to 800/sup 0/F distillate cut. Results from chemical analyses showed the EDS materials, in general, to be more highly alkylated and have higher hydroaromatic content than analogous SRC II process materials (no in-line process hydrogenation) used for comparison. In the microbial mutagenicity assays the N-PAC fractions showed greater activity than did the aliphatic hydrocarbon, hydroxy-PAH, or PAH fractions, although mutagenicity was detected in certain PAH fractions by a modified version of the standard microbial mutagenicity assay. Mutagenic activities for the EDS materials were lower, overall, than those for the corresponding materials from the SRC II process. The EDS materials produced under different operational modes had distinguishable differences in both their chemical constituency and biological activity. The primary differences between the EDS materials studied here and their SRC II counterparts used for comparison are most likely attributable to the incorporation of catalytic hydrogenation in the EDS process. 27 references, 28 figures, 27 tables.

  8. Effect of Job Specialization on the Hospital Stay and Job Satisfaction of ED Nurses.

    Science.gov (United States)

    Shamsi, Vahid; Mahmoudi, Hosein; Sirati Nir, Masoud; Babatabar Darzi, Hosein

    2016-02-01

    In recent decades, the increasing crowdedness of the emergency departments has posed various problems for patients and healthcare systems worldwide. These problems include prolonged hospital stay, patient dissatisfaction and nurse burnout or job dissatisfaction. The aim of this study was to investigate the effect of emergency department (ED) nurses' job specialization on their job satisfaction and the length of patient stay in the ED. This before-after quasi-experimental study was conducted from April to May 2014 at the Baqiyatallah Hospital, Tehran, Iran. Initially, 35 patients were recruited as controls and the length of their stay in the ED was measured in minutes via a chronometer; Moreover, nurses' job satisfaction was evaluated using the Mohrman-Cooke-Mohrman job satisfaction scale. Then, a job specialization intervention was developed based on the stabilization model. After that, 35 new patients were recruited to the treatment group and received specialized care services. Accordingly, the length of their stay in the ED was measured. Moreover, the same nurses' job satisfaction was re-evaluated after the study. The study intervention lasted one month. Data were analyzed using the SPSS software version 20 and statistical tests such as the Kolmogrov-Smirnov, the paired and the independent t, and chi-square tests. There was a significant difference between the two groups of patients concerning the length of their stay in the ED (P nurses had greater job satisfaction after the study (P nurses' satisfaction and relieve the crowdedness of the EDs.

  9. The use of SEM/EDS method in mineralogical analysis of ordinary chondritic meteorite

    Directory of Open Access Journals (Sweden)

    Breda Mirtič

    2009-12-01

    Full Text Available The aim of this study was to evaluate the potential of scanning electron microscopy coupled with energy dispersiveX-ray spectroscopy (SEM/EDS for determination of mineral phases according to their stoichiometry and assessment of mineral composition of ordinary chondritic meteorite. For the purposes of this study, H3 type ordinary chondritic meteorite Abbott was selected. SEM/EDS allows identification and characterisation of mineralphases, whose size is below the resolution of an optical microscope. Mineral phases in chondrules and interstitial matrix were located in backscattered electron (BSE mode and were assessed from atomic proportions of constituent elements, obtained by the EDS analysis. SEM/EDS analyses of mineral phases showed that Abbott meteorite is characterised by Fe-rich (Fe, Ni-alloy kamacite, Fe-sulphide troilite or pyrrhotite, chromite, Mg-rich olivine, orthopyroxene bronzite or hypersthene, clinopyroxene Al-diopside, acid plagioclase oligoclase, accessory mineral chlorapatite and secondary minerals Fe-hydroxides (goethite or lepidocrocite. Results of semi-quantitative analyses confirmed that most of analysed mineralphases conform well to stoichiometric minerals with minor deviations of oxygen from stoichiometric proportions. Comparison between mineral phases in chondrules and interstitial matrix was also performed, however it showed no significant differences in elemental composition.Differences in chemical composition between minerals in interstitial matrix and chondrules are sometimes too small to be discernedby the SEM/EDS, therefore knowledge of SEM/EDS capabilities is important for correct interpretation of chondrite formation.

  10. [Survey on the barriers in providing the mental health services to the suicide attempters in ED].

    Science.gov (United States)

    Zhao, Xiaohui; Li, Tao; Hong, Xia; Jiang, Yinan; Xiong, Nana; Cao, Jinya; Wei, Jing

    2015-06-16

    The purpose of this study was to explore the barriers existing in medical settings behind the phenomenon that few suicide attempters were provided by mental health services when they got treatment in the general hospital ED. An anonymous and voluntary survey was administered among two groups: ED physicians and psychiatrists. Two tertiary general hospitals and one psychiatric hospital were chosen through convenience sampling. Suicide Behavior Attitude Questionnaire and a self-made questionnaire to evaluate barriers were used. The response rate was 68.7% for ED physicians, and 87.0% for psychiatrists. ED physicians generally scored unfavorably than psychiatrists on many items in SBAQ (P suicide attempters should have access to mental health services, the median of the scores = 10 for both group (P = 0.059), meaning highly agree. Some consistent barriers could be summarized from the self-made questionnaire. The majority of ED physicians in general hospitals still hold some misunderstanding about the suicide attempters, but they fully agree that the suicide attempters should have access to mental health services. The barriers for psychiatrists are clear.The barriers for ED physicians are complicated but workable.

  11. C-Terminal Domain Swapping of SSB Changes the Size of the ssDNA Binding Site

    Directory of Open Access Journals (Sweden)

    Yen-Hua Huang

    2014-01-01

    Full Text Available Single-stranded DNA-binding protein (SSB plays an important role in DNA metabolism, including DNA replication, repair, and recombination, and is therefore essential for cell survival. Bacterial SSB consists of an N-terminal ssDNA-binding/oligomerization domain and a flexible C-terminal protein-protein interaction domain. We characterized the ssDNA-binding properties of Klebsiella pneumoniae SSB (KpSSB, Salmonella enterica Serovar Typhimurium LT2 SSB (StSSB, Pseudomonas aeruginosa PAO1 SSB (PaSSB, and two chimeric KpSSB proteins, namely, KpSSBnStSSBc and KpSSBnPaSSBc. The C-terminal domain of StSSB or PaSSB was exchanged with that of KpSSB through protein chimeragenesis. By using the electrophoretic mobility shift assay, we characterized the stoichiometry of KpSSB, StSSB, PaSSB, KpSSBnStSSBc, and KpSSBnPaSSBc, complexed with a series of ssDNA homopolymers. The binding site sizes were determined to be 26±2, 21±2, 29±2, 21±2, and 29±2 nucleotides (nt, respectively. Comparison of the binding site sizes of KpSSB, KpSSBnStSSBc, and KpSSBnPaSSBc showed that the C-terminal domain swapping of SSB changes the size of the binding site. Our observations suggest that not only the conserved N-terminal domain but also the C-terminal domain of SSB is an important determinant for ssDNA binding.

  12. Maximization of Loading and Stability of ssDNA:Iron Oxide Nanoparticle Complexes Formed through Electrostatic Interaction

    Science.gov (United States)

    Berti, Lorenzo; Woldeyesus, Temesgen; Li, Yuanpei; Lam, Kit S.

    2010-01-01

    The use of inorganic nanoparticles (NPs) as vectors for the delivery of oligonucleotides for in vitro and in vivo applications is rapidly gaining momentum. Some of the reasons making them especially good candidates for this purpose are their ease of synthesis in a range of sizes and surface coatings, their propensity to penetrate cell membranes, their stability and biocompatibility and their unique size-dependent physical properties that impart additional diagnostic and therapeutic tools. Notwithstanding these notable attributes, a major obstacle to their practical use is given by the typically low oligonucleotide loading levels attainable through conventional bioconjugation procedures. This shortcoming is especially worrisome as toxicity concerns have been associated with co-delivery of NPs. In this paper we are analytically analyzing the formation of electrostatic complexes between negatively charged ssDNA and positively charged iron oxide nanoparticles (SPIO-NP) with the purpose of identifying the optimal conditions leading to stable formulations at high oligo loading levels. The formation and loading levels of ssDNA:SPIO-NP complexes have been investigated at different oligo:NP ratios and under different ionic strengths through Dynamic Light Scattering, fluorescence quenching experiments and pull-down assays. Through these studies we have identified optimal conditions for attaining maximal oligo loading levels and we are proposing a simple model to explain an unusual behavior observed in the formation of the complexes. Finally we introduce an alternative loading method relying on the electrostatic co-loading of an oligo sequence in the presence of a negatively charged PEGylated block copolymer, yielding very stable and high loading PEGylated ssDNA:SPIO-NPs. The findings that we are reporting are of general validity and similar conditions could be easily translated to the electrostatic formation of ssDNA:NP complexes consisting of different NP materials and

  13. Validation of the flow-through chamber (FTC and steady-state (SS methods for clearance rate measurements in bivalves

    Directory of Open Access Journals (Sweden)

    Poul S. Larsen

    2011-09-01

    To obtain precise and reliable laboratory clearance rate (filtration rate measurements with the ‘flow-through chamber method’ (FTC the design must ensure that only inflow water reaches the bivalve's inhalant aperture and that exit flow is fully mixed. As earlier recommended these prerequisites can be checked by a plot of clearance rate (CR versus increasing through-flow (Fl to reach a plateau, which is the true CR, but we also recommend to plot percent particles cleared versus reciprocal through-flow where the plateau becomes the straight line CR/Fl, and we emphasize that the percent of particles cleared is in itself neither a criterion for valid CR measurement, nor an indicator of appropriate ‘chamber geometry’ as hitherto adapted in many studies. For the ‘steady-state method’ (SS, the design must ensure that inflow water becomes fully mixed with the bivalve's excurrent flow to establish a uniform chamber concentration prevailing at its incurrent flow and at the chamber outlet. These prerequisites can be checked by a plot of CR versus increasing Fl, which should give the true CR at all through-flows. Theoretically, the experimental uncertainty of CR for a given accuracy of concentration measurements depends on the percent reduction in particle concentration (100×P from inlet to outlet of the ideal ‘chamber geomety’. For FTC, it decreases with increasing values of P while for SS it first decreases but then increases again, suggesting the use of an intermediate value of P. In practice, the optimal value of P may depend on the given ‘chamber geometry’. The fundamental differences between the FTC and the SS methods and practical guidelines for their use are pointed out, and new data on CR for the blue mussel, Mytilus edulis, illustrate a design and use of the SS method which may be employed in e.g. long-term growth experiments at constant algal concentrations.

  14. Synthesis of carbon nanotube using camphor with SS 316 as catalytic substrate via oxidative heat treatment preparation method

    Science.gov (United States)

    Wulan, Praswasti Pembangun Dyah Kencana; Angelina, Dian

    2017-11-01

    Carbon nanotube (CNT) is a material that now often become the topic in nanotechnology research. CNT is widely used in the electronics industry especially for TV and computer flat panel displays, devices, automotives for car components, and batteries. Also for defense industries as well as other industries such as sports equipment. Camphor (C10H16O), a botanical hydrocarbon, can be used as a renewable and low cost carbon source for CNT synthesis. Synthesis was performed with stainless steel-316 (SS 316) as substrate, argon as carrier gas, and hydrogen as co-reactant. Preparation of the SS 316 was through a pretreatment by oxidative heat treatment method at a temperature of 850oC for 30 minutes, to remove the layer of chrome and make a rough surface as a growth media for CNT. The operating temperature of the synthesis used was 800oC with a reaction time of 60 minutes. Reactor, which made from stainless steel 316 (SS 316), was used for synthesis CNTs with maximum camphor mass of 20 grams. This research was conducted by varying the number of camphor mass by 5, 7, 10, 12, and 15 grams. The results showed that camphor decomposed into three compounds which are 40% benzene, 8% toluene, and 52% xylene. CNT grows on the surface of the SS 316 plate for each variation. CNTs have grown by follow tips growth model with deformations like buckling growth model and continuous growth model were also founded. The results of XRD showed that CNT were found in every camphor mass variation with high intensity at 2θ angle of 26° and 43°. The best quality and yield of CNT was obtained at camphor mass of 15 grams with carbon percentage of 87,1% and diameter 33 - 44 nm.

  15. Small molecule probes finely differentiate between various ds- and ss-DNA and RNA by fluorescence, CD and NMR response

    Energy Technology Data Exchange (ETDEWEB)

    Crnolatac, Ivo; Rogan, Iva; Majić, Boris; Tomić, Sanja [Division of Organic Chemistry and Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, P.O. Box 180, 10002 Zagreb (Croatia); Deligeorgiev, Todor [Faculty of Chemistry and Pharmacy, University of Sofia (Bulgaria); Horvat, Gordan [Department of Physical Chemistry, Faculty of Science/Chemistry, Horvatovac 102A, HR-10000 Zagreb (Croatia); Makuc, Damjan; Plavec, Janez [Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, Ljubljana (Slovenia); EN-FIST Centre of Excellence, Trg Osvobodilne Fronte 13, Ljubljana (Slovenia); Pescitelli, Gennaro [Department of Chemistry, University of Pisa, Via Moruzzi 13, Pisa (Italy); Piantanida, Ivo, E-mail: pianta@irb.hr [Division of Organic Chemistry and Biochemistry, Division of Physical Chemistry, Ruđer Bošković Institute, P.O. Box 180, 10002 Zagreb (Croatia)

    2016-10-12

    Two small molecules showed intriguing properties of analytical multipurpose probes, whereby one chromophore gives different signal for many different DNA/RNA by application of several highly sensitive spectroscopic methods. Dyes revealed pronounced fluorescence ratiomeric differentiation between ds-AU-RNA, AT-DNA and GC-DNA in approximate order 10:8:1. Particularly interesting, dyes showed specific fluorimetric response for poly rA even at 10-fold excess of any other ss-RNA, and moreover such emission selectivity is preserved in multicomponent ss-RNA mixtures. The dyes also showed specific chiral recognition of poly rU in respect to the other ss-RNA by induced CD (ICD) pattern in visible range (400–500 nm), which was attributed to the dye-side-chain contribution to binding (confirmed by absence of any ICD band for reference compound lacking side-chain). Most intriguingly, minor difference in the side-chain attached to dye chromophore resulted in opposite sign of dye-ICD pattern, whereby differences in NMR NOESY contacts and proton chemical shifts between two dye/oligo rU complexes combined with MD simulations and CD calculations attributed observed bisignate ICD to the dimeric dye aggregate within oligo rU. - Highlights: • Novel dyes emit fluorescence only for poly rA even at high excess of all other ss-RNA. • Fluorescence response for AT-DNA is 8 times stronger than for GC-DNA. • Florescence induced by ds-RNA is 20% stronger that emission induced by ds-DNA. • Intrinsically non-chiral, dyes show strong and characteristic ICD response for poly rU.

  16. Statistical Analysis and Prediction on Tensile Strength of 316L-SS Joints at High Temperature Based on Weibull Distribution

    Science.gov (United States)

    An, Z. L.; Chen, T.; Cheng, D. L.; Chen, T. H.; Y Wang, Z.

    2017-12-01

    In this work, the prediction on average tensile strength of 316L stainless steel is statistically analyzed by Weibull distribution method. Direct diffusion bonding of 316L-SS was performed at high temperature of 550°C and 8 tension tests were carried out. The results obtained vary between 87.8MPa and 160.8MPa. The probability distribution of material failure is obtained by using the Weibull distribution.

  17. Direct Base-to-Base Transitions in ssDNA Revealed by Tip-Enhanced Raman Scattering

    CERN Document Server

    Lin, Xiu-Mei; Singh, Prabha; Siegmann, Michael; Kupfer, Stephan; Zhang, Zhenglong; Gräfe, Stefanie; Deckert, Volker

    2016-01-01

    In the present contribution, specifically designed single-stranded DNA (ssDNA) sequences composed of adenine and cytosine were used as nanometric rulers to target the maximum achievable spatial resolution of tip-enhanced Raman spectroscopy (TERS) under ambient conditions. By stepping along a strand with a TERS tip, the obtained spectra allowed for a clear spectral discrimination including conformational information of the nucleobases, and even sharp adenine-cytosine transitions were detected repeatedly with a spatial resolution below 1 nm.

  18. HAAVAHOITOKOULUTUS LÄHIHOITAJAOPISKELIJOILLE : Koulutuksen suunnittelu, toteutus ja arviointi Sataedun Kankaanpään yksikössä

    OpenAIRE

    Kautto, Jaana

    2012-01-01

    HAAVAHOITOKOULUTUS LÄHIHOITAJAOPISKELIJOILLE. Koulutuksen suunnittelu, toteutus ja arviointi Sataedun Kankaanpään yksikössä Kautto, Jaana Satakunnan ammattikorkeakoulu Terveyden edistämisen koulutusohjelma Marraskuu 2012 Ohjaaja: Hirvonen, Eila Sivumäärä: 54 Liitteitä: 9 Asiasanat: haavahoito, koulutus, lähihoitajaopiskelijat, arviointi _____________________________________________________________ Opinnäyteyön tarkoituksena oli laatia laadukas koulutus- j...

  19. Electrochemical and in vitro bioactivity of polypyrrole/ceramic nanocomposite coatings on 316L SS bio-implants

    Energy Technology Data Exchange (ETDEWEB)

    Madhan Kumar, A. [Center of Research Excellence in Corrosion, King Fahd University of Petroleum and Minerals, Dhahran 31261 (Saudi Arabia); Nagarajan, S. [Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, Tokyo (Japan); Ramakrishna, Suresh [Graduate School of Biomedical Science and Engineering/College of Medicine, Hanyang University, Sungdong-gu, Seoul (Korea, Republic of); Sudhagar, P.; Kang, Yong Soo [Energy Materials Laboratory, Hanyang University, Seoul 133-791 (Korea, Republic of); Kim, Hyongbum [Graduate School of Biomedical Science and Engineering/College of Medicine, Hanyang University, Sungdong-gu, Seoul (Korea, Republic of); Gasem, Zuhair M. [Center of Research Excellence in Corrosion, King Fahd University of Petroleum and Minerals, Dhahran 31261 (Saudi Arabia); Rajendran, N., E-mail: nrajendran@annauniv.edu [Department of Chemistry, Anna University, Chennai (India)

    2014-10-01

    The present investigation describes the versatile fabrication and characterization of a novel composite coating that consists of polypyrrole (PPy) and Nb{sub 2}O{sub 5} nanoparticles. Integration of the two materials is achieved by electrochemical deposition on 316L stainless steel (SS) from an aqueous solution of oxalic acid containing pyrrole and Nb{sub 2}O{sub 5} nanoparticles. Fourier transform infrared spectral (FTIR) and X-ray diffraction (XRD) studies revealed that the existence of Nb{sub 2}O{sub 5} nanoparticles in PPy matrix with hexagonal structure. Surface morphological analysis showed that the presence of Nb{sub 2}O{sub 5} nanoparticles strongly influenced the surface nature of the nanocomposite coated 316L SS. Micro hardness results revealed the enhanced mechanical properties of PPy nanocomposite coated 316L SS due to the addition of Nb{sub 2}O{sub 5} nanoparticles. The electrochemical studies were carried out using cyclic polarization and electrochemical impedance spectroscopy (EIS) measurements. In order to evaluate the biocompatibility, contact angle measurements and in vitro characterization were performed in simulated body fluid (SBF) and on MG63 osteoblast cells. The results showed that the nanocomposite coatings exhibit superior biocompatibility and enhanced corrosion protection performance over 316L SS than pure PPy coatings. - Highlights: • Effect of Nb{sub 2}O{sub 5} nanoparticles on the bio activity of PPy coatings was evaluated. • Hydrophilic, more compact and smooth morphology of nanocomposite was achieved. • Nb{sub 2}O{sub 5} nanoparticles enhanced the corrosion protection performance of PPy coating. • Mechanical and surface wettability of nanocomposite exhibited higher than PPy. • Nano Nb{sub 2}O{sub 5} in PPy coating improved the biocompatibility on osteoblast MG63 cells.

  20. Experimental comparison of pathogenic potential of two sibling species Anisakis simplex s.s. and Anisakis pegreffii in Wistar rat.

    Science.gov (United States)

    del Carmen Romero, María; Valero, Adela; Navarro-Moll, María Concepción; Martín-Sánchez, Joaquina

    2013-08-01

    There are little data available on the pathology caused by the sibling species Anisakis simplex s.s. and Anisakis pegreffii. The differences shown in their ability to penetrate the muscle of fish may also be manifested in humans. The purpose of this study is to confirm possible differences in pathogenicity between A. simplex s.s. and A. pegreffii using an experimental model which simulates infection in humans. Female Wistar rats were infected with 190 Anisakis type I L3 larvae from the Iberian coastline. After the animal was sacrificed, these L3 larvae were then recovered and identified via PCR-RFLP of the ITS1-5.8S-ITS2. A logistic regression analysis was performed searching for association between experimental pathogenic potential and species. The distribution of A. simplex s.s. and A. pegreffii between Atlantic and Mediterranean waters of the Iberian Peninsula showed statistically significant differences (P  0.3). 21.6% showed pathogenic potential, interpreted as the capacity of the larvae to cause lesions, stick to the gastrointestinal wall or penetrate it. The species variable showed association with the pathogenic role of the larva (P = 0.008). Taking A. simplex s.s. as our reference, the OR for A. pegreffii is 0.351 (P = 0.028). Despite this difference, A. pegreffii is also capable of causing anisakiasis, being responsible for 14.3% of the penetrations of the gastric mucosa found in rats, which justifies both species being considered aetiologic agents of this parasitic disorder. © 2013 John Wiley & Sons Ltd.

  1. The KIzSS network, a sentinel surveillance system for infectious diseases in day care centers: study protocol

    Directory of Open Access Journals (Sweden)

    Enserink Remko

    2012-10-01

    Full Text Available Abstract Background Day care-associated infectious diseases are widely recognized as a public health problem but rarely studied. Insights into their dynamics and their association with the day care setting are important for effective decision making in management of infectious disease control. This paper describes the purpose, design and potential of our national multi-center, day care-based sentinel surveillance network for infectious diseases (the KIzSS network. The aim of the KIzSS network is to acquire a long-term insight into the syndromic and microbiological aspects of day care-related infectious diseases and associated disease burden and to model these aspects with day care setting characteristics. Methods/design The KIzSS network applies a prospective cohort design, following day care centers rather than individual children or staff members over time. Data on infectious disease symptoms and related morbidity (children and staff, medical consumption, absenteeism and circulating enteric pathogens (children are collected on a daily, weekly or monthly basis. Every two years, a survey is performed to assess the characteristics of participating day care centers. Discussion The KIzSS network offers a unique potential to study infectious disease dynamics in the day care setting over a sustained period of time. The created (biodatabases will help us to assess day care-related disease burden of infectious diseases among attending children and staff and their relation with the day care setting. This will support the much needed development of evidence-based and pragmatic guidelines for infectious disease control in day care centers.

  2. Extensive FE-SEM/EDS, HR-TEM/EDS and ToF-SIMS studies of micron- to nano-particles in anthracite fly ash.

    Science.gov (United States)

    Ribeiro, Joana; DaBoit, Kátia; Flores, Deolinda; Kronbauer, Marcio A; Silva, Luis F O

    2013-05-01

    The generation of anthropogenic carbonaceous matter and mixed crystalline/amorphous mineral ultrafine/nano-particles in the 1 to 100 nm size range by worldwide coal power plants represents serious environmental problems due to their potential hazards. Coal fly ash (CFA) that resulted from anthracite combustion in a Portuguese thermal power plant was studied in this work. The physico-chemical characterization of ultrafine/nano-particles present in the CFA samples and their interaction with environment are the aim of this study. The methodologies applied for this work were field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray spectroscopy (EDS), high-resolution transmission electron microscopy with energy dispersive X-ray spectroscopy (HR-TEM/EDS) and time of flight secondary ion mass spectrometry (ToF-SIMS). Some hazardous volatile elements, C, N, S and Hg contents were also determined in the studied samples. Generally, the CFA samples comprise carbonaceous, glassy and metallic solid spheres with some containing mixed amorphous/crystalline phases. The EDS analysis coupled with the FE-SEM and HR-TEM observations of the fly ash particles with 100 to 0.1 nm demonstrates that these materials contain a small but significant proportion of encapsulated HVEs. In addition, the presence of abundant multi-walled carbon nanotubes (MWCNTs) and amorphous carbon particles, both containing hazardous volatile elements (HVEs), was also evidenced by the FE-SEM/EDS and HR-TEM/EDS analysis. A wide range of organic and inorganic compounds was determined by chemical maps obtained in ToF-SIMS analysis. Copyright © 2013 Elsevier B.V. All rights reserved.

  3. Extensive FE-SEM/EDS, HR-TEM/EDS and ToF-SIMS studies of micron- to nano-particles in anthracite fly ash

    Energy Technology Data Exchange (ETDEWEB)

    Ribeiro, Joana [Centro de Geologia, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto (Portugal); DaBoit, Kátia [Institute of Environmental Research and Human Development, IPADHC, Capivari de Baixo, Santa Catarina (Brazil); Flores, Deolinda [Centro de Geologia, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto (Portugal); Departamento de Geociências, Ambiente e Ordenamento do Território, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto (Portugal); Kronbauer, Marcio A. [Laboratory of Environmental Researches and Nanotechnology Development, Centro Universitário La Salle, Victor Barreto, 2288 Centro 92010-000, Canoas, RS (Brazil); Silva, Luis F.O., E-mail: felipeqma@hotmail.com [Laboratory of Environmental Researches and Nanotechnology Development, Centro Universitário La Salle, Victor Barreto, 2288 Centro 92010-000, Canoas, RS (Brazil); Environmental Science and Nanotechnology Department, Catarinense Institute of Environmental Research and Human Development, IPADHC, Capivari de Baixo, Santa Catarina (Brazil)

    2013-05-01

    The generation of anthropogenic carbonaceous matter and mixed crystalline/amorphous mineral ultrafine/nano-particles in the 1 to 100 nm size range by worldwide coal power plants represents serious environmental problems due to their potential hazards. Coal fly ash (CFA) that resulted from anthracite combustion in a Portuguese thermal power plant was studied in this work. The physico-chemical characterization of ultrafine/nano-particles present in the CFA samples and their interaction with environment are the aim of this study. The methodologies applied for this work were field emission scanning electron microscopy (FE-SEM) with energy dispersive X-ray spectroscopy (EDS), high-resolution transmission electron microscopy with energy dispersive X-ray spectroscopy (HR-TEM/EDS) and time of flight secondary ion mass spectrometry (ToF-SIMS). Some hazardous volatile elements, C, N, S and Hg contents were also determined in the studied samples. Generally, the CFA samples comprise carbonaceous, glassy and metallic solid spheres with some containing mixed amorphous/crystalline phases. The EDS analysis coupled with the FE-SEM and HR-TEM observations of the fly ash particles with 100 to 0.1 nm demonstrates that these materials contain a small but significant proportion of encapsulated HVEs. In addition, the presence of abundant multi-walled carbon nanotubes (MWCNTs) and amorphous carbon particles, both containing hazardous volatile elements (HVEs), was also evidenced by the FE-SEM/EDS and HR-TEM/EDS analysis. A wide range of organic and inorganic compounds was determined by chemical maps obtained in ToF-SIMS analysis. - Highlights: ► We examine changes in the level of ultrafine and nanoparticles of coal mining. ► Increasing geochemical information will increase human health information in this area. ► Electron bean and Tof-SIMS increase area information.

  4. How reliable is MRCP with an SS-FSE sequence at 3.0 T: comparison between SS-FSE BH and 3D-FSE BH ASSET sequences.

    Science.gov (United States)

    Lavdas, Eleftherios; Vlychou, Marianna; Arikidis, Nikolaos; Kapsalaki, Eftychia; Roka, Violeta; Arvanitis, Dimitrios L; Fezoulidis, Ioannis; Vassiou, Katerina

    2013-01-01

    The purpose of the present study was to evaluate the visibility and the image quality of the biliary and pancreatic duct system on magnetic resonance cholangiopancreatography (MRCP) images based on two breath-hold (BH) methods using array spatial sensitivity technique: a single-shot fast spin-echo (SS-FSE) sequence and a three-dimensional single slab fast spin-echo (3D-FSE) sequence. In the present prospective comparative study, 47 patients (22 male and 25 female, mean age=50 years, age range=22-82 years) that were referred for MRCP during a 12-month period are included. All of them were referred with suspected pancreaticobiliary disease. All patients underwent MRCP with both a SS-FSE BH sequence and a 3D-FSE BH sequence. Qualitative evaluation regarding the depiction of three segments of the pancreaticobiliary tree and the frequency of artifacts was performed. Two radiologists graded each sequence of the obtained studies in a blinded fashion. Quantitative evaluation including calculation of relative signal intensity (rSI) and relative contrast (RC) ratios at seven segments of the pancreaticobiliary tree between fluid-filled ductal structures and organ parenchyma at the same ductal segments was performed. In order to evaluate the parameters' differences of the two sequences, either in qualitative or in quantitative analysis, the Wilcoxon paired signed-rank test was performed. On quantitative evaluation, both rSI and RC ratios of all segments of the pancreaticobiliary tree at SS-FSE BH sequence were higher than those at 3D-FSE BH sequences. This finding was statistically significant (P0.60) between the two radiologists. The SS-FSE sequence is performed faster and significantly improves image quality; thus, it should be included into the routine MRCP sequence protocol at 3.0 T. Furthermore, we recommended SS-FSE BH MRCP examination to be applied to uncooperative patients or patients in emergency because of its short acquisition time (1 s). Copyright © 2013 Elsevier

  5. Amyotrophic Lateral Sclerosis Survival Score (ALS-SS): A simple scoring system for early prediction of patient survival.

    Science.gov (United States)

    Lunetta, Christian; Lizio, Andrea; Melazzini, Mario Giovanni; Maestri, Eleonora; Sansone, Valeria A

    2015-01-01

    Our objectives were: (1) to identify independent prognostic factors to determine a survival score for amyotrophic lateral sclerosis (ALS) in a cohort of patients followed in the NEMO Centre (NEuroMuscular Omnicentre); (2) to replicate results in an independent cohort obtained from the Pooled Resource Open Access ALS Clinical Trial Consortium (PRO-ACT) database. Samples were collected from 428 ALS patients from the NEMO database and 2481 patients from the PRO-ACT database. Study design was a retrospective analysis with clinical and biochemical variables, using univariable and multivariable Cox models of analysis. Results showed that, in multivariable analysis, age at diagnosis, diagnostic delay, ALSFRS-R total score, Body Mass Index, aspartate aminotransferase and creatinine level were independently related to survival. These factors were recoded as categorical variables assigning a score from 5 to 15, and the sums of these scores were used to obtain the ALS-Survival Score (ALS-SS). This then allowed to identify three groups having different survival curves. The ALS-SS results were also replicated using data from the PRO-ACT database. In conclusion, considering independent prognostic factors, we were able to give an estimate of survival in our cohort of ALS patients. Whether this ALS-SS may be useful in clinical practice, and potentially in clinical trials, will have to be determined prospectively.

  6. Experimental study for determining the corrosion in situ of SS-304 in heat transformers applying electrochemical techniques

    Energy Technology Data Exchange (ETDEWEB)

    Cuevas-Arteaga, C.; Siqueiros, J.; Romero, R.J. [Centro de Investigacion en Ingenieria y Ciencias Aplicadas - UAEM, Cuernavaca, Morelos (Mexico)

    2009-07-01

    In this work the corrosion performance of SS-304 experimentally tested in a single stage absorption heat transformer (SSAHT) operating with lithium-bromide aqueous solution and water as the absorbent and work fluids coupled to a desalinizer is reported. The main aim of this work was to determine the corrosion rate and corrosion mechanism of SS-304 through some electrochemical cells designed for this specific purpose and connected in one of the pipe constituting the heat transformer. Some electrochemical techniques named electrochemical noise (EN), and electrochemical impedance spectroscopy (EIS) were applied under the next experimental conditions: temperature 83-85 C, concentration of LiBr-H{sub 2}O corrosive solution 56 (wt.%), and flow rate 0.0278 m{sup 3}/s. According to the physical characterization, SS-304 suffered a mixed corrosion process, which is in concordance with the signal noise pattern obtained. Through the Nyquist plots from EIS the corrosion process was obtained as controlled by charge transfer at the first 3:25 h, and then a diffusion effect was observed. (authors)

  7. Whole genome sequence of the Treponema pallidum subsp. pallidum strain Amoy: An Asian isolate highly similar to SS14.

    Science.gov (United States)

    Tong, Man-Li; Zhao, Qiang; Liu, Li-Li; Zhu, Xiao-Zhen; Gao, Kun; Zhang, Hui-Lin; Lin, Li-Rong; Niu, Jian-Jun; Ji, Zhi-Liang; Yang, Tian-Ci

    2017-01-01

    Treponema pallidum ssp. pallidum (T. pallidum), the causative agent of the sexually transmitted disease syphilis, is an uncultivatable human pathogen. The geographical differences in T. pallidum genomes leading to differences in pathogenicity are not yet understood. Presently, twelve T. pallidum genomes are available to the public, all of which are American in origin and often co-infect patients with human immunodeficiency virus (HIV). In this study, we examined the T. pallidum subsp. pallidum strain Amoy, a syphilis pathogen found in Xiamen, China. We sequenced its genome using Illumina next-generation sequencing technology and obtained a nearly (98.83%) complete genome of approximately 1.12 Mbps. The new genome shows good synteny with its five T. pallidum sibling strains (Nichols, SS14, Mexico A, DAL-1, and Chicago), among which SS14 is the strain closest to the Amoy strain. Compared with strain SS14, the Amoy strain possesses four uncharacterized strain-specific genes and is likely missing six genes, including a gene encoding the TPR domain protein, which may partially account for the comparatively low virulence and toxicity of the Amoy strain in animal infection. Notably, we did not detect the 23S rRNA A2058G/A2059G mutation in the Amoy strain, which likely explains the sensitivity of Amoy strain to macrolides. The results of this study will lead to a better understanding of the pathogenesis of syphilis and the geographical distribution of T. pallidum genotypes.

  8. A novel silica nanotube reinforced ionic incorporated hydroxyapatite composite coating on polypyrrole coated 316L SS for implant application.

    Science.gov (United States)

    Prem Ananth, K; Joseph Nathanael, A; Jose, Sujin P; Oh, Tae Hwan; Mangalaraj, D

    2016-02-01

    An attempt has been made to deposit a novel smart ion (Sr, Zn, Mg) substituted hydroxyapatite (I-HAp) and silica nanotube (SiNTs) composite coatings on polypyrrole (PPy) coated surgical grade 316L stainless steel (316L SS) to improve its biocompatibility and corrosion resistance. The I-HAp/SiNTS/PPy bilayer coating on 316L SS was prepared by electrophoretic deposition technique. Potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) studies were carried out. These results confirmed the significant improvement of the corrosion resistance of the 316L SS alloy by the I-HAp/SiNTs/PPy bilayer composite coating. The adhesion strength and hardness test confirmed the anticipated mechanical properties of the composite. A low contact angle value revealed the hydrophilic nature. Inductively coupled plasma-atomic emission spectroscopy (ICP-AES) was used for the leach out analysis of the samples. Added to this, the bioactivity of the composite was analyzed by observing the apatite formation in the SBF solution for 7, 14, 21 and 28days of incubation. An enhancement of in vitro osteoblast attachment and cell viability was observed, which could lead to the optimistic orthopedic and dental applications. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Production of $\\eta$ mesons in 200 AGeV S+S and S+Au reactions

    CERN Document Server

    Albrecht, R.; Awes, T.C.; Barlag, C.; Berger, F.; Bloomer, M.; Blume, C.; Bock, D.; Bock, R.; Bohne, E.M.; Bucher, D.; Claussen, A.; Clewing, G.; Debbe, R.; Dragon, L.; Eklund, A.; Fokin, S.; Garpman, S.; Glasow, R.; Gustafsson, H.A.; Gutbrod, H.H.; Hansen, O.; Holker, G.; Idh, J.; Ippolitov, M.; Jacobs, P.; Kampert, K.H.; Karadev, K.; Kolb, B.W.; Lebedev, A.; Lohner, H.; Lund, I.; Manko, V.; Moskowitz, B.; Nikolaev, S.; Obenshain, F.E.; Oskarsson, A.; Otterlund, I.; Peitzmann, T.; Plasil, F.; Poskanzer, Arthur M.; Purschke, M.; Roters, B.; Santo, R.; Schmidt, H.R.; Soderstrom, K.; Sorensen, S.P.; Stankus, P.; Steffens, K.; Steinhauser, P.; Stenlund, E.; Stuken, D.; Vinogradov, A.; Wegner, H.E.; Young, G.R.

    1995-01-01

    Minimum Bias production cross sections of $\\eta$ mesons have been measured in 200AGeV S+Au and S+S collisions at the CERN SPS by reconstructing the $\\eta\\rightarrow\\gamma\\gamma$ decay. The measurements have been made over the rapidity range $2.1 \\leq y \\leq 2.9$ using the leadglass spectrometer of WA80. Within the statistical and systematical uncertainties the spectral shapes of $\\pi~0$ and $\\eta$ mesons yields are identical when their invariant differential cross section is plotted as a function of the transverse mass. The relative normalization of the $\\eta$ to $\\pi~0$ transverse mass spectra is found to be $0.53 \\pm 0.07$ for S+Au and $0.43 \\pm 0.15$ for S+S reactions. Extrapolation to full phase space leads to an integrated cross section ratio of $\\eta$ to $\\pi~0$ mesons of $0.15 \\pm 0.02 {\\rm (stat.)} \\pm 0.02 {\\rm (syst.)}$, and $0.12 \\pm 0.03 {\\rm (stat.)} \\pm 0.02 {\\rm (syst.)}$ for S+Au and S+S collisions, respectively.

  10. Combination treatments with the PKC inhibitor, enzastaurin, enhance the cytotoxicity of the anti-mesothelin immunotoxin, SS1P.

    Directory of Open Access Journals (Sweden)

    Abid R Mattoo

    Full Text Available Activated protein kinase C (PKC contributes to tumor survival and proliferation, provoking the development of inhibitory agents as potential cancer therapeutics. Immunotoxins are antibody-based recombinant proteins that employ antibody fragments for cancer targeting and bacterial toxins as the cytotoxic agent. Pseudomonas exotoxin-based immunotoxins act via the ADP-ribosylation of EF2 leading to the enzymatic inhibition of protein synthesis. Combining PKC inhibitors with the immunotoxin SS1P, targeted to surface mesothelin, was undertaken to explore possible therapeutic strategies. Enzastaurin but not two other PKC inhibitors combined with SS1P to produce synergistic cell death via apoptosis. Mechanistic insights of the synergistic killing centered on the complete loss of the prosurvival Bcl2 protein, Mcl-1, the loss of AKT and the activation of caspase 3/7. Synergy was most evident when cells exhibited resistance to the immunotoxin alone. Further, because PKC inhibition by itself was not sufficient to enhance SS1P action, enzastaurin must target other kinases that are involved in the immunotoxin pathway.

  11. Novel secretory protein Ss-Caf1 of the plant-pathogenic fungus Sclerotinia sclerotiorum is required for host penetration and normal sclerotial development.

    Science.gov (United States)

    Xiao, Xueqiong; Xie, Jiatao; Cheng, Jiasen; Li, Guoqing; Yi, Xianhong; Jiang, Daohong; Fu, Yanping

    2014-01-01

    To decipher the mechanism of pathogenicity in Sclerotinia sclerotiorum, a pathogenicity-defective mutant, Sunf-MT6, was isolated from a T-DNA insertional library. Sunf-MT6 could not form compound appressorium and failed to induce lesions on leaves of rapeseed though it could produce more oxalic acid than the wild-type strain. However, it could enter into host tissues via wounds and cause typical necrotic lesions. Furthermore, Sunf-MT6 produced fewer but larger sclerotia than the wild-type strain Sunf-M. A gene, named Ss-caf1, was disrupted by T-DNA insertion in Sunf-MT6. Gene complementation and knockdown experiments confirmed that the disruption of Ss-caf1 was responsible for the phenotypic changes of Sunf-MT6. Ss-caf1 encodes a secretory protein with a putative Ca(2+)-binding EF-hand motif. High expression levels of Ss-caf1 were observed at an early stage of compound appressorium formation and in immature sclerotia. Expression of Ss-caf1 without signal peptides in Nicotiana benthamiana via Tobacco rattle virus-based vectors elicited cell death. These results suggest that Ss-caf1 plays an important role in compound appressorium formation and sclerotial development of S. sclerotiorum. In addition, Ss-Caf1 has the potential to interact with certain host proteins or unknown substances in host cells, resulting in subsequent host cell death.

  12. The chromosomal SezAT toxin-antitoxin system promotes the maintenance of the SsPI-1 pathogenicity island in epidemic Streptococcus suis.

    Science.gov (United States)

    Yao, Xinyue; Chen, Tian; Shen, Xiaodong; Zhao, Yan; Wang, Min; Rao, Xiancai; Yin, Supeng; Wang, Jing; Gong, Yali; Lu, Shuguang; Le, Shuai; Tan, Yinling; Tang, Jiaqi; Fuquan, Hu; Li, Ming

    2015-10-01

    Streptococcus suis has emerged as a causative agent of human meningitis and streptococcal toxic shock syndrome over the last years. The high pathogenicity of S. suis may be due in part to a laterally acquired pathogenicity island (renamed SsPI-1), which can spontaneously excise and transfer to recipients. Cells harboring excised SsPI-1 can potentially lose this island if cell division occurs prior to its reintegration; however, attempts to cure SsPI-1 from the host cells have been unsuccessful. Here, we report that an SsPI-1-borne Epsilon/Zeta toxin-antitoxin system (designated SezAT) promotes SsPI-1 stability in bacterial populations. The sezAT locus consists of two closely linked sezT and sezA genes encoding a toxin and its cognate antitoxin, respectively. Overproduction of SezT induces a bactericidal effect that can be neutralized by co-expression of SezA, but not by its later action. When devoid of a functional SezAT system, large-scale deletion of SsPI-1 is straightforward. Thus, SezAT serves to ensure inheritance of SsPI-1 during cell division, which may explain the persistence of epidemic S. suis. This report presents the first functional characterization of TA loci in S. suis, and the first biochemical evidence for the adaptive significance of the Epsilon/Zeta system in the evolution of pathogen virulence. © 2015 John Wiley & Sons Ltd.

  13. SS-Wrapper: a package of wrapper applications for similarity searches on Linux clusters

    Directory of Open Access Journals (Sweden)

    Lefkowitz Elliot J

    2004-10-01

    Full Text Available Abstract Background Large-scale sequence comparison is a powerful tool for biological inference in modern molecular biology. Comparing new sequences to those in annotated databases is a useful source of functional and structural information about these sequences. Using software such as the basic local alignment search tool (BLAST or HMMPFAM to identify statistically significant matches between newly sequenced segments of genetic material and those in databases is an important task for most molecular biologists. Searching algorithms are intrinsically slow and data-intensive, especially in light of the rapid growth of biological sequence databases due to the emergence of high throughput DNA sequencing techniques. Thus, traditional bioinformatics tools are impractical on PCs and even on dedicated UNIX servers. To take advantage of larger databases and more reliable methods, high performance computation becomes necessary. Results We describe the implementation of SS-Wrapper (Similarity Search Wrapper, a package of wrapper applications that can parallelize similarity search applications on a Linux cluster. Our wrapper utilizes a query segmentation-search (QS-search approach to parallelize sequence database search applications. It takes into consideration load balancing between each node on the cluster to maximize resource usage. QS-search is designed to wrap many different search tools, such as BLAST and HMMPFAM using the same interface. This implementation does not alter the original program, so newly obtained programs and program updates should be accommodated easily. Benchmark experiments using QS-search to optimize BLAST and HMMPFAM showed that QS-search accelerated the performance of these programs almost linearly in proportion to the number of CPUs used. We have also implemented a wrapper that utilizes a database segmentation approach (DS-BLAST that provides a complementary solution for BLAST searches when the database is too large to fit into

  14. Molecular and Functional Characterization of ssDNA Aptamers that Specifically Bind Leishmania infantum PABP

    Science.gov (United States)

    Guerra-Pérez, Natalia; Ramos, Edurne; García-Hernández, Marta; Pinto, Celia; Soto, Manuel; Martín, M. Elena; González, Víctor M.

    2015-01-01

    Summary A poly (A)-binding protein from Leishmania infantum (LiPABP) has been recently cloned and characterized in our laboratory. Although this protein shows a very high homology with PABPs from other eukaryotic organisms including mammals and other parasites, exist divergences along the sequence that convert them in potential diagnostic markers and/or therapeutics targets. Aptamers are oligonucleotide ligands that are selected in vitro by their affinity and specificity for the target as a consequence of the particular tertiary structure that they are able to acquire depending on their sequence. Development of high-affinity molecules with the ability to recognize specifically Leishmania proteins is essential for the progress of this kind of study. Results We have selected a ssDNA aptamer population against a recombinant 6xHIS–LiPABP protein (rLiPABP) that is able to recognize the target with a low Kd. Cloning, sequencing and in silico analysis of the aptamers obtained from the population yielded three aptamers (ApPABP#3, ApPABP#7 and ApPABP#11) that significantly bound to PABP with higher affinity than the naïve population. These aptamers were analyzed by ELONA and slot blot to establish affinity and specificity for rLiPABP. Results demonstrated that the three aptamers have high affinity and specificity for the target and that they are able to detect an endogenous LiPABP (eLiPABP) protein amount corresponding to 2500 L. infantum promastigotes in a significant manner. The functional analysis of the aptamers also revealed that ApPABP#11 disrupts the binding of both Myc-LiPABP and eLiPABP to poly (A) in vitro. On the other hand, these aptamers are able to bind and purify LiPABP from complex mixes. Conclusion Results presented here demonstrate that aptamers represent new reagents for characterization of LiPABP and that they can affect LiPABP activity. At this respect, the use of these aptamers as therapeutic tool affecting the physiological role of PABP has to be

  15. Auv Multibeam Bathymetry and Sidescan Survey of the SS Montebello wreck Offshore Cambria CA

    Science.gov (United States)

    Caress, D. W.; Thomas, H.; Conlin, D.; Thompson, D.; Paull, C. K.

    2010-12-01

    An MBARI Mapping AUV survey of the SS Montebello wreck offshore Cambria, CA collected high-resolution multibeam bathymetry and sidescan imagery of the vessel and the surrounding seafloor. The Montebello was an oil tanker that was torpedoed and sunk about 11 km offshore in 275 m water depth by a Japanese submarine on December 23, 1941. The Montebello was loaded with 3,000,000 gallons of crude oil, and there is no evidence that significant leakage of that cargo occurred at the time of the sinking or in the 69 years since. The California Department of Fish and Game’s Office of Spill Prevention and Response (OSPR) commissioned the AUV survey as part of a multi-agency Montebello Task Force effort to assess the potential pollution threat. The survey data will be used to determine the extent and general character of the wreckage for a pending Task Force report and to guide any future ROV dive or assessment activity . The AUV surveyed the wreck site from altitudes of 75 and 25 m; the low-altitude high-resolution survey consists of a grid with a 50 m line spacing both parallel and orthogonal to the ship. The 200 kHz multibeam bathymetry images the wreck from both above and from the sides with an 0.5 m lateral resolution. The combination of soundings from all of the survey lines results in a three-dimensional distribution of soundings that delineates the external morphology and some of the internal structure of the wreck. 410 kHz chirp sidescan sonar data also image the site from both directions. The bathymetry data indicate that the Montebello was pitched forward down when it impacted the bottom, crushing and breaking off the bow section. Both forward and aft deckhouses are largely intact, and in fact the multibeam images the individual decks within those structures. About half of the forward mast remains, both amidships masts and the smokestack are missing. A good deal of the deck piping and equipment appears intact, and aside from the bow, the ship’s sides appear

  16. SS-Wrapper: a package of wrapper applications for similarity searches on Linux clusters.

    Science.gov (United States)

    Wang, Chunlin; Lefkowitz, Elliot J

    2004-10-28

    Large-scale sequence comparison is a powerful tool for biological inference in modern molecular biology. Comparing new sequences to those in annotated databases is a useful source of functional and structural information about these sequences. Using software such as the basic local alignment search tool (BLAST) or HMMPFAM to identify statistically significant matches between newly sequenced segments of genetic material and those in databases is an important task for most molecular biologists. Searching algorithms are intrinsically slow and data-intensive, especially in light of the rapid growth of biological sequence databases due to the emergence of high throughput DNA sequencing techniques. Thus, traditional bioinformatics tools are impractical on PCs and even on dedicated UNIX servers. To take advantage of larger databases and more reliable methods, high performance computation becomes necessary. We describe the implementation of SS-Wrapper (Similarity Search Wrapper), a package of wrapper applications that can parallelize similarity search applications on a Linux cluster. Our wrapper utilizes a query segmentation-search (QS-search) approach to parallelize sequence database search applications. It takes into consideration load balancing between each node on the cluster to maximize resource usage. QS-search is designed to wrap many different search tools, such as BLAST and HMMPFAM using the same interface. This implementation does not alter the original program, so newly obtained programs and program updates should be accommodated easily. Benchmark experiments using QS-search to optimize BLAST and HMMPFAM showed that QS-search accelerated the performance of these programs almost linearly in proportion to the number of CPUs used. We have also implemented a wrapper that utilizes a database segmentation approach (DS-BLAST) that provides a complementary solution for BLAST searches when the database is too large to fit into the memory of a single node. Used together

  17. Synthesis and antioxidant evaluation of (S,S)- and (R,R)-secoisolariciresinol diglucosides (SDGs).

    Science.gov (United States)

    Mishra, Om P; Simmons, Nicholas; Tyagi, Sonia; Pietrofesa, Ralph; Shuvaev, Vladimir V; Valiulin, Roman A; Heretsch, Philipp; Nicolaou, K C; Christofidou-Solomidou, Melpo

    2013-10-01

    Secoisolariciresinol diglucosides (SDGs) (S,S)-SDG-1 (major isomer in flaxseed) and (R,R)-SDG-2 (minor isomer in flaxseed) were synthesized from vanillin via secoisolariciresinol (6) and glucosyl donor 7 through a concise route that involved chromatographic separation of diastereomeric diglucoside derivatives (S,S)-8 and (R,R)-9. Synthetic (S,S)-SDG-1 and (R,R)-SDG-2 exhibited potent antioxidant properties (EC50=292.17±27.71 μM and 331.94±21.21 μM, respectively), which compared well with that of natural (S,S)-SDG-1 (EC50=275.24±13.15 μM). These values are significantly lower than those of ascorbic acid (EC50=1129.32±88.79 μM) and α-tocopherol (EC50=944.62±148.00 μM). Compounds (S,S)-SDG-1 and (R,R)-SDG-2 also demonstrated powerful scavenging activities against hydroxyl [natural (S,S)-SDG-1: 3.68±0.27; synthetic (S,S)-SDG-1: 2.09±0.16; synthetic (R,R)-SDG-2: 1.96±0.27], peroxyl [natural (S,S)-SDG-1: 2.55±0.11; synthetic (S,S)-SDG-1: 2.20±0.10; synthetic (R,R)-SDG-2: 3.03±0.04] and DPPH [natural (S,S)-SDG-1: EC50=83.94±2.80 μM; synthetic (S,S)-SDG-1: EC50=157.54±21.30 μM; synthetic (R,R)-SDG-2: EC50=123.63±8.67 μM] radicals. These results confirm previous studies with naturally occurring (S,S)-SDG-1 and establish both (S,S)-SDG-1 and (R,R)-SDG-2 as potent antioxidants and free radical scavengers for potential in vivo use. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Resequencing of Treponema pallidum ssp. pallidum strains Nichols and SS14: correction of sequencing errors resulted in increased separation of syphilis treponeme subclusters.

    Directory of Open Access Journals (Sweden)

    Helena Pětrošová

    Full Text Available BACKGROUND: Treponema pallidum ssp. pallidum (TPA, the causative agent of syphilis, is a highly clonal bacterium showing minimal genetic variability in the genome sequence of individual strains. Nevertheless, genetically characterized syphilis strains can be clearly divided into two groups, Nichols-like strains and SS14-like strains. TPA Nichols and SS14 strains were completely sequenced in 1998 and 2008, respectively. Since publication of their complete genome sequences, a number of sequencing errors in each genome have been reported. Therefore, we have resequenced TPA Nichols and SS14 strains using next-generation sequencing techniques. METHODOLOGY/PRINCIPAL FINDINGS: The genomes of TPA strains Nichols and SS14 were resequenced using the 454 and Illumina sequencing methods that have a combined average coverage higher than 90x. In the TPA strain Nichols genome, 134 errors were identified (25 substitutions and 109 indels, and 102 of them affected protein sequences. In the TPA SS14 genome, a total of 191 errors were identified (85 substitutions and 106 indels and 136 of them affected protein sequences. A set of new intrastrain heterogenic regions in the TPA SS14 genome were identified including the tprD gene, where both tprD and tprD2 alleles were found. The resequenced genomes of both TPA Nichols and SS14 strains clustered more closely with related strains (i.e. strains belonging to same syphilis treponeme subcluster. At the same time, groups of Nichols-like and SS14-like strains were found to be more distantly related. CONCLUSION/SIGNIFICANCE: We identified errors in 11.5% of all annotated genes and, after correction, we found a significant impact on the predicted proteomes of both Nichols and SS14 strains. Corrections of these errors resulted in protein elongations, truncations, fusions and indels in more than 11% of all annotated proteins. Moreover, it became more evident that syphilis is caused by treponemes belonging to two separate genetic

  19. Glutathione-Sensitive Hyaluronic Acid-SS-Mertansine Prodrug with a High Drug Content: Facile Synthesis and Targeted Breast Tumor Therapy.

    Science.gov (United States)

    Zhong, Ping; Zhang, Jian; Deng, Chao; Cheng, Ru; Meng, Fenghua; Zhong, Zhiyuan

    2016-11-14

    Low tolerability and tumor selectivity restricts many potent anticancer drugs including mertansine from wide clinical use. Here, glutathione-activatable hyaluronic acid-SS-mertansine prodrug (HA-SS-DM1) was designed and developed to achieve enhanced tolerability and targeted therapy of CD44+ human breast tumor xenografts. DM1 was readily conjugated to HA using 2-(2-pyridyldithio)-ethylamine as a linker. Notably, HA-SS-DM1 with a high DM1 content of 20 wt % had a mean size of ∼170 nm at concentrations above 0.2 mg/mL while transformed into unimers upon dilution to 0.04 mg/mL. HA-SS-DM1 exhibited a superior targetability to MCF-7 cancer cells with an exceptionally low IC50 of 0.13 μg DM1/mL. The pharmacokinetic studies displayed that Cy5-labeled HA-SS-DM1 had an elimination half-life of 2.12 h. Notably, HA-SS-DM1 displayed better tolerability with a maximum-tolerated dose 4-fold higher than free DM1. Cy5-labeled HA-SS-DM1 quickly accumulated in the MCF-7 tumor, the fluorescence intensity of which was maximized at 24 h post injection and kept strong in 48 h. The tumor Cy5 level reached 8.17%ID/g at 24 h. The therapeutic results demonstrated that HA-SS-DM1 effectively inhibited tumor growth at 800 μg DM1 equiv/kg while causing reduced side effects as compared to free DM1. Glutathione-cleavable HA-SS-DM1 prodrug with superior drug content, excellent targetability, enhanced tolerability, and easy large-scale synthesis appears to be a highly promising alternative to clinically used Trastuzumab emtansine (T-DM1) for targeted breast tumor therapy.

  20. Burnout syndrome among dental students: a short version of the "Burnout Clinical Subtype Questionnaire" adapted for students (BCSQ-12-SS

    Directory of Open Access Journals (Sweden)

    Montero-Marin Jesus

    2011-12-01

    Full Text Available Abstract Background Burnout has been traditionally defined in relation to the dimensions of "exhaustion", "cynicism", and "inefficiency". More recently, the Burnout Clinical Subtype Questionnaire (BCSQ-12 further established three different subtypes of burnout: the "frenetic" subtype (related to "overload", the "under-challenged" subtype (related to "lack of development", and the "worn-out" subtype (related to "neglect". However, to date, these definitions have not been applied to students. The aims of this research were (1 to adapt a Spanish version of the BCSQ-12 for use with students, (2 to test its factorial validity, internal consistency, convergent and discriminant validity, and (3 to assess potential socio-demographic and occupational risk factors associated with the development of the subtypes. Method We used a cross-sectional design on a sample of dental students (n = 314 from Santiago and Huesca universities (Spain. Participants completed the Burnout Clinical Subtype Questionnaire Student Survey (BCSQ-12-SS, the Maslach Burnout Inventory Student Survey (MBI-SS, and a series of socio-demographic and occupational questions formulated for the specific purpose of this study. Data were subjected to exploratory factor analysis (EFA using the principal component method with varimax orthogonal rotation. To assess the relations with the criterion, we calculated the Pearson correlation coefficient (r, multiple correlation coefficient (Ry.123, and the coefficient of determination (R2y.123. To assess the association between the subtypes and the socio-demographic variables, we examined the adjusted odds ratio (OR obtained from multivariate logistic regression models. Results Factorial analyses supported the theoretical proposition of the BCSQ-12-SS, with α-values exceeding 0.80 for all dimensions. The "overload-exhaustion" relation was r = 0.59 (p y.123 = 0.62, 30.25% in "cynicism" (Ry.123 = 0.55, and 26.01% in "inefficiency" (Ry.123 = 0

  1. Postmenopausal Increase in KiSS-1, GPR54, and Luteinizing Hormone Releasing Hormone (LHRH-1) mRNA in the Basal Hypothalamus of Female Rhesus Monkeys

    OpenAIRE

    Kim, Wooram; Jessen, Heather M; Auger, Anthony P; Terasawa, Ei

    2008-01-01

    The G-protein coupled receptor, GPR54, and its ligand, kisspeptin-54 (a KiSS-1 derived peptide) have been reported to be important players in control of LHRH-1 release. However, the role of the GPR54 signaling in primate reproductive senescence is still unclear. In the present study we investigated whether KiSS-1, GPR54, and LHRH-1 mRNA in the brain change after menopause in female rhesus monkeys using quantitative real-time PCR. Results indicate that KiSS-1, GPR54, and LHRH-1 mRNA levels in ...

  2. Potential of computer-aided diagnosis of high spectral and spatial resolution (HiSS) MRI in the classification of breast lesions.

    Science.gov (United States)

    Bhooshan, Neha; Giger, Maryellen; Medved, Milica; Li, Hui; Wood, Abbie; Yuan, Yading; Lan, Li; Marquez, Angelica; Karczmar, Greg; Newstead, Gillian

    2014-01-01

    To compare the performance of computer-aided diagnosis (CADx) analysis of precontrast high spectral and spatial resolution (HiSS) MRI to that of clinical dynamic contrast-enhanced MRI (DCE-MRI) in the diagnostic classification of breast lesions. Thirty-four malignant and seven benign lesions were scanned using two-dimensional (2D) HiSS and clinical 4D DCE-MRI protocols. Lesions were automatically segmented. Morphological features were calculated for HiSS, whereas both morphological and kinetic features were calculated for DCE-MRI. After stepwise feature selection, Bayesian artificial neural networks merged selected features, and receiver operating characteristic (ROC) analysis evaluated the performance with leave-one-lesion-out validation. AUC (area under the ROC curve) values of 0.92 ± 0.06 and 0.90 ± 0.05 were obtained using CADx on HiSS and DCE-MRI, respectively, in the task of classifying benign and malignant lesions. While we failed to show that the higher HiSS performance was significantly better than DCE-MRI, noninferiority testing confirmed that HiSS was not worse than DCE-MRI. CADx of HiSS (without contrast) performed similarly to CADx on clinical DCE-MRI; thus, computerized analysis of HiSS may provide sufficient information for diagnostic classification. The results are clinically important for patients in whom contrast agent is contra-indicated. Even in the limited acquisition mode of 2D single slice HiSS, by using quantitative image analysis to extract characteristics from the HiSS images, similar performance levels were obtained as compared with those from current clinical 4D DCE-MRI. As HiSS acquisitions become possible in 3D, CADx methods can also be applied. Because HiSS and DCE-MRI are based on different contrast mechanisms, the use of the two protocols in combination may increase diagnostic accuracy. Copyright © 2013 Wiley Periodicals, Inc.

  3. Improved Early Detection of Sepsis in the ED With a Novel Monocyte Distribution Width Biomarker.

    Science.gov (United States)

    Crouser, Elliott D; Parrillo, Joseph E; Seymour, Christopher; Angus, Derek C; Bicking, Keri; Tejidor, Liliana; Magari, Robert; Careaga, Diana; Williams, JoAnna; Closser, Douglas R; Samoszuk, Michael; Herren, Luke; Robart, Emily; Chaves, Fernando

    2017-09-01

    Sepsis most often presents to the ED, and delayed detection is harmful. WBC count is often used to detect sepsis, but changes in WBC count size also correspond to sepsis. We sought to determine if volume increases of circulating immune cells add value to the WBC count for early sepsis detection in the ED. A blinded, prospective cohort study was conducted in two different ED populations within a large academic hospital. Neutrophil and monocyte volume parameters were measured in conjunction with routine CBC testing on a UniCel DxH 800 analyzer at the time of ED admission and were evaluated for the detection of sepsis. There were 1,320 subjects in the ED consecutively enrolled and categorized as control subjects (n = 879) and those with systemic inflammatory response syndrome (SIRS) (n = 203), infection (n = 140), or sepsis (n = 98). Compared with other parameters, monocyte distribution width (MDW) best discriminated sepsis from all other conditions (area under the curve [AUC], 0.79; 95% CI, 0.73-0.84; sensitivity, 0.77; specificity, 0.73; MDW threshold, 20.50), sepsis from SIRS (AUC, 0.74; 95% CI, 0.67-0.84), and severe sepsis from noninfected patients in the ED (AUC, 0.88; 95% CI, 0.75-0.99; negative predictive value, 99%). The added value of MDW to WBC count was statistically significant (AUC, 0.89 for MDW + WBC vs 0.81 for WBC alone; P sepsis compared with WBC count alone at the time of admission in the ED. ClinicalTrials.gov; No.: NCT02232750; URL: www.clinicaltrials.gov. Copyright © 2017 American College of Chest Physicians. Published by Elsevier Inc. All rights reserved.

  4. Trends in Hospital Admission and Surgical Procedures Following ED visits for Diverticulitis.

    Science.gov (United States)

    Greenwood-Ericksen, Margaret B; Havens, Joaquim M; Ma, Jiemin; Weissman, Joel S; Schuur, Jeremiah D

    2016-07-01

    Diverticulitis is a common diagnosis in the emergency department (ED). Outpatient management of diverticulitis is safe in selected patients, yet the rates of admission and surgical procedures following ED visits for diverticulitis are unknown, as are the predictive patient characteristics. Our goal is to describe trends in admission and surgical procedures following ED visits for diverticulitis, and to determine which patient characteristics predict admission. : We performed a cross-sectional descriptive analysis using data on ED visits from 2006-2011 to determine change in admission and surgical patterns over time. The Nationwide Emergency Department Sample database, a nationally representative administrative claims dataset, was used to analyze ED visits for diverticulitis. We included patients with a principal diagnosis of diverticulitis (ICD-9 codes 562.11, 562.13). We analyzed the rate of admission and surgery in all admitted patients and in low-risk patients, defined as age diverticulitis. Fryom 2006 to 2011 ED visits for diverticulitis increased by 21.3% from 238,248 to 302,612, while the admission rate decreased from 55.7% to 48.5% (-7.2%, 95% CI [-7.78 to -6.62]; pdiverticulitis was independently associated with male gender, comorbid illnesses, higher income and commercial health insurance. The surgical rate decreased from 6.5% in 2006 to 4.7% in 2011 (-1.8%, 95% CI [-2.1 to -1.5]; pdiverticulitis increased, while ED admission rates and surgical rates declined, with comorbidity, sociodemographic factors predicting hospitalization. Future work should focus on determining if these differences reflect increased disease prevalence, increased diagnosis, or changes in management.

  5. Prevalence of Diabetes Mellitus (DM) in a population of men affected by Erectile Dysfunction (ED).

    Science.gov (United States)

    Mazzilli, R; Elia, J; Delfino, M; Benedetti, F; Scordovillo, G; Mazzilli, F

    2015-01-01

    A) to evaluate the prevalence of patients affected by Diabetes Mellitus (DM) in a population of men with Erectile Dysfunction (ED); B) to define the epidemiological, biochemical and therapeutic aspects. N.934 subjects referred at our Andrology Unit for ED were studied. The diagnosis of ED was evaluated using the IIEF-5 questionnaire (Total score ≤21). The prevalence of subjects affected by DM in a population of men with ED was 19.5% (182/934). The age ranges were: ≥55 years (108/182; 59.3%); ≥40diabetic treatment. In n.125/182 cases (68.7%) the ED onset followed the diagnosis of DM; in n.34/182 cases (18.7%) it appeared at the same time; and in n.23/182 cases (12.6%) appeared before DM diagnosis. in n.18/182 subjects (9.9%) there was a concomitant hypotestosteronemia; these patients were treated only with testosterone replacement; this treatment was efficacious (IIEF-5 total score ≥22) in 8/18 subjects (44.4%). In n.146/182 subjects (80.2%) a treatment with PDE5-i was given. Of these 146 subjects, the therapy was given "on demand" to 108 subjects (efficacy in 50.9%; 55/108) and "once a day" to the remaining 38 subjects (efficacy 63.1%, 24/38) (p=0.428, n.s.). N.15/182 subjects (8.2%) were treated with intracavernous injections of Alprostadil (efficacy in 8/15, 53.3%). In n.3/182 subjects (1.6%) a penile prosthesis was implanted. DM is one of the most frequent organic causes of ED; there were many strategies to treat this symptom without interfering with the antidiabetic treatment. Finally, ED can be predictive of DM.

  6. Suicide Prevention in an Emergency Department Population: The ED-SAFE Study.

    Science.gov (United States)

    Miller, Ivan W; Camargo, Carlos A; Arias, Sarah A; Sullivan, Ashley F; Allen, Michael H; Goldstein, Amy B; Manton, Anne P; Espinola, Janice A; Jones, Richard; Hasegawa, Kohei; Boudreaux, Edwin D

    2017-06-01

    Suicide is a leading cause of deaths in the United States. Although the emergency department (ED) is an opportune setting for initiating suicide prevention efforts, ED-initiated suicide prevention interventions remain underdeveloped. To determine whether an ED-initiated intervention reduces subsequent suicidal behavior. This multicenter study of 8 EDs in the United States enrolled adults with a recent suicide attempt or ideation and was composed of 3 sequential phases: (1) a treatment as usual (TAU) phase from August 2010 to December 2011, (2) a universal screening (screening) phase from September 2011 to December 2012, and (3) a universal screening plus intervention (intervention) phase from July 2012 to November 2013. Screening consisted of universal suicide risk screening. The intervention phase consisted of universal screening plus an intervention, which included secondary suicide risk screening by the ED physician, discharge resources, and post-ED telephone calls focused on reducing suicide risk. The primary outcome was suicide attempts (nonfatal and fatal) over the 52-week follow-up period. The proportion and total number of attempts were analyzed. A total of 1376 participants were recruited, including 769 females (55.9%) with a median (interquartile range) age of 37 (26-47) years. A total of 288 participants (20.9%) made at least 1 suicide attempt, and there were 548 total suicide attempts among participants. There were no significant differences in risk reduction between the TAU and screening phases (23% vs 22%, respectively). However, compared with the TAU phase, patients in the intervention phase showed a 5% absolute reduction in suicide attempt risk (23% vs 18%), with a relative risk reduction of 20%. Participants in the intervention phase had 30% fewer total suicide attempts than participants in the TAU phase. Negative binomial regression analysis indicated that the participants in the intervention phase had significantly fewer total suicide attempts

  7. Triage quality control is missing tools-a new observation technique for ED quality improvement.

    Science.gov (United States)

    Malmström, Tomi; Harjola, Veli-Pekka; Torkki, Paulus; Kumpulainen, Salla; Malmström, Raija

    2017-04-01

    Correct assessment of patient urgency is critical to ensuring patient safety in emergency departments (EDs). Although significant time and effort have been devoted to developing triage systems, less attention has been paid to the development of quality control. The aim of this study is to introduce and test observation technique, which enables identifying of patient groups at risk of erroneous assessment in triage. The introduced technique is aimed to be less laborious to use than existing triage quality control methods. The study developed an observation technique for identifying patients with possible erroneous assessments in triage. Data sample for the observation technique is carried out with survey form filled in by nurse. Hospital ED with ~74 000 patient visits annually. Consecutive adult patients in an ED for baseline study period of 14 days (1774 patients) in 2010 and control study period of 4 days (541 patients) in 2012. Triage observation technique for continuous improvement of triage performance. Primary measures of triage improvement were triage accuracy and nurses' ability to predict patient admissions. With the observation technique the ED staff was able to identify patient groups at risk for erroneous triage. Under-triage related mostly to patients with chest pain, shortness of breath, collapse, stomach pain and infections. Instead injures and muscular skeletal symptoms were seldom undertriaged even though they are common. EDs can control triage quality with simple observation technique. The usability of observation technique and triage quality improvement process were good.

  8. The Relationship Between the Use of a Worksite Medical Home and ED Visits or Hospitalizations

    Directory of Open Access Journals (Sweden)

    Marissa Stroo BS

    2015-10-01

    Full Text Available Worksite medical homes may be a good model for improving employee health. The aim of this study was to compare the likelihood of being seen in the emergency department (ED or being hospitalized by level of use (no use, occasional use, or primary care of a worksite medical home, overall and by type of user (employee, adult dependent, or pediatric dependent. This was a retrospective analysis of claims data, using covariate-adjusted logistic regression models for ED visits and inpatient hospitalizations. Secondary data for the years 2006 to 2008 from a company that offers an on-site health care center (HCC were used. Analyses were based on a data set that combines health plan claims and human resources demographic data. Overall, people who did not use the HCC were more likely to be seen in the ED (adjusted odds ratio [OR] = 1.20, 95% confidence interval or CI [1.06, 1.37], P = .005 or to be hospitalized (adjusted OR = 1.58; 95% CI [1.34, 1.86]; P < .0001 compared with those who used the HCC for primary care. Both ED visits and hospitalizations for employees and dependents in this study were lower among those who used the worksite medical home for primary care. Worksite medical homes can improve chronic disease management and thus reduce ED visits and hospitalizations. These findings contribute to growing evidence that worksite medical homes are potentially cost-effective.

  9. Factors affecting stress in emergency medicine residents while working in the ED.

    Science.gov (United States)

    Wrenn, Keith; Lorenzen, Brent; Jones, Ian; Zhou, Chuan; Aronsky, Dominik

    2010-10-01

    The aim of this study was to identify factors other than work hours in the emergency department (ED) work environment contributing to resident stress. This study involved a prospective cohort evaluation of emergency medicine residents in the ED. Twelve surveys were collected from 18 subjects, 4 each from the day, evening, and night shifts. The Perceived Stress Questionnaire and a visual analog stress scale were administered. Data collected included the shift number of a given consecutive sequence of shifts, number of procedures performed, number of adverse events, average age of the patients seen by the resident, triage nurse-assigned acuities of the patients seen by the resident during the shift, the number of patients seen during a shift, the number of patients admitted by the resident during the shift, anticipated overtime after a shift, and shift-specific metrics related to overcrowding, including average waiting room time both for the individual residents and for all patients, average waiting room count for all patients, and average occupancy of the ED for all patients. Among the 216 studied shifts, there was considerable variability in stress both within and between residents. In the multivariate mixed-effect regression analysis, only anticipated overtime and process failures were correlated with stress. Factors related to ED overcrowding had no significant effect on resident stress. Resident stress was most impacted by anticipation of overtime and adverse events. Overcrowding in the ED and traditional measures of workload did not seem to affect stress as much. Copyright © 2010 Elsevier Inc. All rights reserved.

  10. Hardiness Mediates Stress and Impact Level in ED Nurses Who Experienced a Violent Event.

    Science.gov (United States)

    Park, Jin Hee; Lee, Eun Nam; Kong, Kyung Ran; Jang, Moon Jung

    2017-11-01

    This secondary analysis examined the mediating effect of hardiness between stress and impact level in ED nurses who experienced a violent event. This correlational study was conducted from June to August 2014. We used the visual analog scale to measure stress level, the Impact of Event Scale-Revised to measure impact level after the violent event, and the Dispositional Resilience Scale to measure hardiness. We then analyzed mediating effects with the Sobel test. Data were collected in 31 emergency medical centers located in B city in Korea. Data from 321 ED nurses who experienced a violent event were analyzed. Most nurses (91.9%) were women, with a mean age of 28.73 years. The main outcome measure was the mediating effect of hardiness between stress and impact level after ED nurses experienced violence. We found that both violence-related stress (B = 0.22, P level from a violent event. Based on results of a Sobel test, hardiness partially mediated the relationship between violence-related stress and impact level from a violent event (Z = 2.03, P = .044). Hardiness had an effect on reducing the impact level of ED nurses who had experienced a violent event and had a mediating role in mitigating their stress. Therefore, we recommend the development of an intervention program that emphasizes the improvement of hardiness in ED nurses. Copyright © 2017 Emergency Nurses Association. Published by Elsevier Inc. All rights reserved.

  11. Mini-KiSS Online: an Internet-based intervention program for parents of young children with sleep problems - influence on parental behavior and children's sleep

    National Research Council Canada - National Science Library

    Schlarb, Angelika A; Brandhorst, Isabel

    2012-01-01

    .... Therefore, in the current study we examined the acceptance and efficacy of a newly developed Internet-based intervention program called Mini-KiSS Online for sleep disturbances for children aged 6...

  12. Operations and Maintenance Manual for Expanded Bioventing System, Site SS-41, Former Building Number 93 (Fuel Pumping Station Number 3), Charleston Air Force Base, Charleston, South Carolina

    National Research Council Canada - National Science Library

    1997-01-01

    This Operations and Maintenance (O&M) Manual has been created as a guide for monitoring and maintaining the performance of the expanded bioventing blower systems and vent well plumbing at Site SS-41 (Former Building...

  13. Effects of KiSS-1 Peptide, the Natural Ligand of GPR54, on Follicle-Stimulating Hormone Secretion in the Rat

    National Research Council Canada - National Science Library

    Navarro, V. M; Castellano, J. M; Fernández-Fernández, R; Tovar, S; Roa, J; Mayen, A; Barreiro, M. L; Casanueva, F. F; Aguilar, E; Dieguez, C; Pinilla, L; Tena-Sempere, M

    2005-01-01

    ... are able to inhibit tumor progression. Unexpectedly, a reproductive facet of this newly discovered system has recently arisen, and characterization of the role of the KiSS-1/GPR54 system in the neuroendocrine control of gonadotropin...

  14. Presence of T3SS2β genes in trh⁺ Vibrio parahaemolyticus isolated from seafood harvested along Mangalore coast, India.

    Science.gov (United States)

    Kumar, B K; Deekshit, V K; Rai, P; Shekar, M; Karunasagar, I; Karunasagar, I

    2014-05-01

    Vibrio parahaemolyticus is a seafood-borne pathogen autochthonous to the marine and estuarine ecosystem, responsible for gastroenteritis when contaminated raw seafood is consumed. The pathogenicity has been associated with thermostable direct haemolysin (TDH) and TDH-related haemolysin (TRH). Of late, the presence of T3SS2α and T3SS2β gene clusters has been well documented in clinical isolates of Vibrio parahaemolyticus and known to play an essential role in pathogenesis. However, reports on the presence of T3SSβ genes in V. parahaemolyticus isolated from the seafood and/or environmental samples are scanty. In this study, we have identified and analysed the distribution of the T3SS2β genes in V. parahaemolyticus isolated from seafood harvested along southwest coast of India. Results showed that T3SS2β genes are solely associated with trh⁺ and tdh⁺ /trh⁺ strains of V. parahaemolyticus. Reverse transcriptase PCR (RT-PCR) showed that the T3SS2β genes identified in trh⁺ V. parahaemolyticus were transcriptionally active. To our knowledge, this study appears to be the first description on the presence of T3SS2β-positive V. parahaemolyticus isolated from seafood in India. The study of T3SS2 along with other virulence factors will help in better understanding of the risk of seafood-borne illness due to V. parahaemolyticus. T3SSs (α or β) are the important virulence factors of Vibrio parahaemolyticus that contribute to their pathogenicity in humans. This study demonstrated the presence of T3SS2β genes in V. parahaemolyticus isolated from the seafood harvested along Mangalore coast. RT-PCR showed that the T3SS2β genes identified in seafood isolates of V. parahaemolyticus were found to be functional. To the best of our knowledge, this is the first description of T3SS2β genes in trh⁺ V. parahaemolyticus isolated from seafood in India. The presence of T3SS2 along with other virulence factors such as TDH and/or TRH highlights a potential health risk for

  15. Differential Ovarian Expression of KiSS-1 and GPR-54 During the Estrous Cycle and Photoperiod Induced Recrudescence in Siberian Hamsters (Phodopus sungorus)

    Science.gov (United States)

    Shahed, Asha; Young, Kelly A.

    2008-01-01

    Kisspeptins, coded by the KiSS-1 gene, regulate aspects of the reproductive axis by stimulating GnRH release via the G protein coupled receptor, GPR54. Recent reports show that KiSS/GPR54 may be key mediators in photoperiod-controlled reproduction in seasonal breeders, and that KiSS-1/GPR54 are expressed in the hypothalamus, ovaries, placenta, and pancreas. This study examined the expression of KiSS-1/GPR54 mRNA and protein in ovaries of Siberian hamsters (Phodopus sungorus). Ovaries from cycling hamsters were collected during proestrus (P), estrus (E), diestrus I (DI), and diestrus II (DII). To examine KiSS-1/GPR54 during stimulated recrudescence, additional hamsters were maintained either in long day (LD 16L:8D, control) or short day (SD 8L:16D) for 14 weeks and then transferred to LD for 0–8 weeks. Staining of KiSS-1/GPR54 protein was detected by immunohistochemistry in steroidogenic cells of preantral and antral follicles, and corpora lutea. Immunostaining peaked in P and E, but decreased in the diestrus stages (pGPR54 immunostaining was low after 14 wks of SD exposure (post transfer [PT] wk0), and increased during the early weeks of recrudescence. Expression of KiSS-1/GPR54 mRNA was low with short day exposure, but increased during recrudescence and was higher at PT wk8 as compared to PTwks 0 and 2 (pGPR54 expression during P and E suggests a potential role in ovulation in Siberian hamsters. Transient increases in KiSS-1/GPR54 expression following LD stimulation are also suggestive of possible involvement in ovulation and/or restoration of ovarian function. PMID:18937338

  16. Type 3 Secretion System (T3SS of Bradyrhizobium sp. DOA9 and Its Roles in Legume Symbiosis and Rice Endophytic Association

    Directory of Open Access Journals (Sweden)

    Pongpan Songwattana

    2017-09-01

    Full Text Available The Bradyrhizobium sp. DOA9 strain isolated from a paddy field has the ability to nodulate a wide spectrum of legumes. Unlike other bradyrhizobia, this strain has a symbiotic plasmid harboring nod, nif, and type 3 secretion system (T3SS genes. This T3SS cluster contains all the genes necessary for the formation of the secretory apparatus and the transcriptional activator (TtsI, which is preceded by a nod-box motif. An in silico search predicted 14 effectors putatively translocated by this T3SS machinery. In this study, we explored the role of the T3SS in the symbiotic performance of DOA9 by evaluating the ability of a T3SS mutant (ΩrhcN to nodulate legumes belonging to Dalbergioid, Millettioid, and Genistoid tribes. Among the nine species tested, four (Arachis hypogea, Vigna radiata, Crotalaria juncea, and Macroptilium atropurpureum responded positively to the rhcN mutation (ranging from suppression of plant defense reactions, an increase in the number of nodules and a dramatic improvement in nodule development and infection, one (Stylosanthes hamata responded negatively (fewer nodules and less nitrogen fixation and four species (Aeschynomene americana, Aeschynomene afraspera, Indigofera tinctoria, and Desmodium tortuosum displayed no phenotype. We also tested the role of the T3SS in the ability of the DOA9 strain to endophytically colonize rice roots, but detected no effect of the T3SS mutation, in contrast to what was previously reported in the Bradyrhizobium SUTN9-2 strain. Taken together, these data indicate that DOA9 contains a functional T3SS that interferes with the ability of the strain to interact symbiotically with legumes but not with rice.

  17. Type 3 Secretion System (T3SS) of Bradyrhizobium sp. DOA9 and Its Roles in Legume Symbiosis and Rice Endophytic Association.

    Science.gov (United States)

    Songwattana, Pongpan; Noisangiam, Rujirek; Teamtisong, Kamonluck; Prakamhang, Janpen; Teulet, Albin; Tittabutr, Panlada; Piromyou, Pongdet; Boonkerd, Nantakorn; Giraud, Eric; Teaumroong, Neung

    2017-01-01

    The Bradyrhizobium sp. DOA9 strain isolated from a paddy field has the ability to nodulate a wide spectrum of legumes. Unlike other bradyrhizobia, this strain has a symbiotic plasmid harboring nod, nif, and type 3 secretion system (T3SS) genes. This T3SS cluster contains all the genes necessary for the formation of the secretory apparatus and the transcriptional activator (TtsI), which is preceded by a nod-box motif. An in silico search predicted 14 effectors putatively translocated by this T3SS machinery. In this study, we explored the role of the T3SS in the symbiotic performance of DOA9 by evaluating the ability of a T3SS mutant (ΩrhcN) to nodulate legumes belonging to Dalbergioid, Millettioid, and Genistoid tribes. Among the nine species tested, four (Arachis hypogea, Vigna radiata, Crotalaria juncea, and Macroptilium atropurpureum) responded positively to the rhcN mutation (ranging from suppression of plant defense reactions, an increase in the number of nodules and a dramatic improvement in nodule development and infection), one (Stylosanthes hamata) responded negatively (fewer nodules and less nitrogen fixation) and four species (Aeschynomene americana, Aeschynomene afraspera, Indigofera tinctoria, and Desmodium tortuosum) displayed no phenotype. We also tested the role of the T3SS in the ability of the DOA9 strain to endophytically colonize rice roots, but detected no effect of the T3SS mutation, in contrast to what was previously reported in the Bradyrhizobium SUTN9-2 strain. Taken together, these data indicate that DOA9 contains a functional T3SS that interferes with the ability of the strain to interact symbiotically with legumes but not with rice.

  18. Patient-centered transfer process for patients admitted through the ED boosts satisfaction, improves safety.

    Science.gov (United States)

    2013-02-01

    To improve safety and patient flow, administrators at Hallmark Health System, based in Melrose, MA, implemented a new patient-centered transfer process for patients admitted through the ED at the health system's two hospitals. Under the new approach, inpatient nurses come down to the ED to take reports on new patients in a process that includes the ED care team as well as family members. The inpatient nurses then accompany the patients up to their designated floors. Since the new patient-transfer process was implemented in June 2012, patient satisfaction has increased by at least one point on patient satisfaction surveys. Administrators anticipate that medical errors or omissions related to the handoff process will show a drop of at least 50%, when data is tabulated.

  19. "Etica ed Estetica sono tutt’uno" Riflessioni su TLP 6.421

    Directory of Open Access Journals (Sweden)

    Gabriele Tomasi

    2011-01-01

    Full Text Available Per il primo Wittgenstein etica ed estetica erano tutt’uno. Scopo del saggio è fornire un’interpretazione di questa concezione. Esaminando il modo in cui è proposta nel Tractatus e considerando alcune annotazioni dei Quaderni 1914-1916 si evidenzia che l’unità di etica ed estetica è in un modo di vedere il mondo per cui esso non appare come fonte di limitazione. L’etica è un’estensione al mondo - alla vita - della capacità di conferire significato che nell’arte si realizza nei riguardi di oggetti particolari. Affermando l’unità di etica ed estetica Wittgenstein attira l’attenzione sul fatto che la radice dell’etica è in un certo modo di vedere le cose, in un atteggiamento verso la vita. Si tratta della prospettiva di un valore non connesso a come il mondo è e che è evocato dalla meraviglia per l’esistenza del mondo.

  20. Impact of 40 years of technology advances on EDS system performance.

    Science.gov (United States)

    McCarthy, Jon; Friel, John; Camus, Patrick

    2009-12-01

    The rapid advance and falling costs of computing power and data storage during the last 40 years have greatly enhanced the data reduction speed and analytical capacity of energy dispersive spectroscopy (EDS) systems. At the same time, the solid state X-ray detector [until recently, the Si(Li) diode] has seen performance increases due to the use of advanced materials and processing techniques. In addition, the performance of the electronic components (field effect transistor, preamp, and pulse processor) of an EDS system has been constantly improved. These technology advances have resulted in improved spectral quality, excellent light element detection, and increased count rate performance. The results have been truly remarkable and driven by the needs of the analyst. This article will summarize the progress made in these areas in the last 40 years and make a brief reference to prospects for future development in EDS system performance.

  1. Benzodiazepine-opioid co-prescribing in a national probability sample of ED encounters.

    Science.gov (United States)

    Kim, Howard S; McCarthy, Danielle M; Mark Courtney, D; Lank, Patrick M; Lambert, Bruce L

    2017-03-01

    Benzodiazepine-opioid combination therapy is potentially harmful due to the risk of synergistic respiratory depression, and the rate of death due to benzodiazepine-opioid overdose is increasing. Little is known about the prevalence and characteristics of benzodiazepine-opioid co-prescribing from the ED setting. Secondary analysis of data from the National Hospital Ambulatory Medical Care Survey, using sample weights to generate population estimates. The primary objective was to describe the annual prevalence of benzodiazepine-opioid co-prescribing from 2006 to 2012, using 95% confidence intervals (95% CI) to compare adjacent years. The secondary objective was to compare characteristics of ED encounters receiving a benzodiazepine-opioid co-prescription versus those receiving an opioid prescription alone, using a multivariable logistic regression. The prevalence of benzodiazepine-opioid co-prescribing did not significantly change from 2006 to 2012. During this period, 2.7% (95% CI: 2.5-2.8%) of ED encounters prescribed an opioid were also prescribed a benzodiazepine. Relative to encounters receiving an opioid prescription alone, encounters receiving a co-prescription were more likely to represent a follow-up rather than initial visit (Odds Ratio [OR] 1.52), receive more medications (OR 1.41) and fewer procedures (OR 0.48) while in the ED, and more likely to have a diagnosis related to mental disorder (OR 20.60) or musculoskeletal problem (OR 3.71). From 2006 to 2012, almost 3% of all ED encounters receiving an opioid prescription also received a benzodiazepine co-prescription. The odds of benzodiazepine-opioid co-prescribing were significantly higher in ED encounters representing a follow-up visit and in diagnoses relating to a mental disorder or musculoskeletal problem. Copyright © 2016 Elsevier Inc. All rights reserved.

  2. Multidisciplinary intervention decreases the use of opioid medication discharge packs from 2 urban EDs.

    Science.gov (United States)

    Gugelmann, Hallam; Shofer, Frances S; Meisel, Zachary F; Perrone, Jeanmarie

    2013-09-01

    Prescription opioid overdoses and deaths constitute a public health epidemic, and recent studies show that emergency department (ED) prescribers may contribute to this crisis. We hypothesized that a multidisciplinary educational intervention would decrease ED opioid packs dispensed at discharge. This prospective study implemented a "bundle" of interdisciplinary educational modalities: lectures, journal clubs, case discussions, and an electronic medical record decision support tool. Implementation occurred in 2 urban EDs in the same health system at different times ("affiliate," September 2011; "primary," January 2012) to better distinguish its effects. The primary outcome was preintervention/postintervention change in opioid discharge packs dispensed to all patients treated and discharged through August 2012 and was assessed by 2-way analysis of variance. The secondary outcome was bivariate analysis (using Fisher exact test) of change in opioid dispensing among patients with known risk factors for prescription opioid dependence: age less than 65 years, history of substance abuse, chronic pain, or psychiatric disorders. A total of 71,512 and 45,746 patients were evaluated and discharged from primary and affiliate EDs, respectively. Orders for opioid discharge packs decreased from 13.9% to 8.4% and 4.7% to 1.9% at the primary and affiliate hospitals (P opioid dependence at the primary ED decreased from 21.8% to 13.9%. A staged, multidisciplinary intervention targeting nurses, residents, nurse practitioners, and attending physicians was associated with decreased orders for opioid discharge packs in 2 urban EDs. Opioid discharge pack orders decreased slightly more among patients with risk factors for prescription opioid dependence. © 2013.

  3. PopED lite: An optimal design software for preclinical pharmacokinetic and pharmacodynamic studies.

    Science.gov (United States)

    Aoki, Yasunori; Sundqvist, Monika; Hooker, Andrew C; Gennemark, Peter

    2016-04-01

    Optimal experimental design approaches are seldom used in preclinical drug discovery. The objective is to develop an optimal design software tool specifically designed for preclinical applications in order to increase the efficiency of drug discovery in vivo studies. Several realistic experimental design case studies were collected and many preclinical experimental teams were consulted to determine the design goal of the software tool. The tool obtains an optimized experimental design by solving a constrained optimization problem, where each experimental design is evaluated using some function of the Fisher Information Matrix. The software was implemented in C++ using the Qt framework to assure a responsive user-software interaction through a rich graphical user interface, and at the same time, achieving the desired computational speed. In addition, a discrete global optimization algorithm was developed and implemented. The software design goals were simplicity, speed and intuition. Based on these design goals, we have developed the publicly available software PopED lite (http://www.bluetree.me/PopED_lite). Optimization computation was on average, over 14 test problems, 30 times faster in PopED lite compared to an already existing optimal design software tool. PopED lite is now used in real drug discovery projects and a few of these case studies are presented in this paper. PopED lite is designed to be simple, fast and intuitive. Simple, to give many users access to basic optimal design calculations. Fast, to fit a short design-execution cycle and allow interactive experimental design (test one design, discuss proposed design, test another design, etc). Intuitive, so that the input to and output from the software tool can easily be understood by users without knowledge of the theory of optimal design. In this way, PopED lite is highly useful in practice and complements existing tools. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  4. Biochemical Analysis of CagE: A VirB4 Homologue of Helicobacter pylori Cag-T4SS.

    Directory of Open Access Journals (Sweden)

    Mohd Shariq

    Full Text Available Helicobacter pylori are among the most successful human pathogens that harbour a distinct genomic segment called cag Pathogenicity Island (cag-PAI. This genomic segment codes for a type IV secretion system (Cag-T4SS related to the prototypical VirB/D4 system of Agrobacterium tumefaciens (Ag, a plant pathogen. Some of the components of Cag-T4SS share homology to that of VirB proteins including putative energy providing CagE (HP0544, the largest VirB4 homologue. In Ag, VirB4 is required for the assembly of the system, substrate translocation and pilus formation, however, very little is known about CagE. Here we have characterised the protein biochemically, genetically, and microscopically and report that CagE is an inner membrane associated active NTPase and has multiple interacting partners including the inner membrane proteins CagV and Cagβ. Through CagV it is connected to the outer membrane sub-complex proteins. Stability of CagE is not dependent on several of the cag-PAI proteins tested. However, localisation and stability of the pilus associated CagI, CagL and surface associated CagH are affected in its absence. Stability of the inner membrane associated energetic component Cagβ, a VirD4 homologue seems to be partially affected in its absence. Additionally, CagA failed to cross the membrane barriers in its absence and no IL-8 induction is observed under infection condition. These results thus suggest the importance of CagE in Cag-T4SS functions. In future it may help in deciphering the mechanism of substrate translocation by the system.

  5. Corrosion behavior of mild steel and SS 304L in presence of dissolved nickel under aerated and deaerated conditions

    Directory of Open Access Journals (Sweden)

    Mohd Mobin

    2011-12-01

    Full Text Available In dual purpose water/power co-generation plants, the presence of high concentration of Cu and Ni in the re-circulating brine/condensate as a result of condenser tubes corrosion has been attributed as one of the several causes of corrosion damage of flash chamber materials and water touched parts of the boilers. The present investigation deals with the effect of dissolved nickel in the concentration range of 10 ppb to 100 ppm on the corrosion behavior of mild steel and SS 304L in two aqueous medium namely, distilled water and artificial seawater. The effect of pH, dissolved oxygen and flow condition of aqueous medium on the corrosion behavior was also monitored. The experimental techniques include immersion test and electrochemical tests which include free corrosion potential measurements and potentiodynamic polarization measurements. The corrosion rate of mild steel and SS 304L under different experimental conditions was determined by weight loss method and spectrophotometric determination of iron ion entered into the test solution during the period of immersion. The pH of the test solution was also monitored during the entire period of immersion. The left over nickel ions present in the test solution after completion of immersion was also estimated using Atomic Absorption Spectrophotometer. The surface morphology of the corroded steel surface was also examined using scanning electron microscopy (SEM. The results of the studies show that SS 304L largely remains unaffected in both distilled water and artificial seawater under different experimental conditions. However, the effect of nickel on the corrosion behavior of mild steel is quite pronounced and follows interesting trends.

  6. Identification of ssDNA aptamers specific to clinical isolates of Streptococcus mutans strains with different cariogenicity.

    Science.gov (United States)

    Cui, Wei; Liu, Jiaojiao; Su, Donghua; Hu, Danyang; Hou, Shuai; Hu, Tongnan; Yang, Jiyong; Luo, Yanping; Xi, Qing; Chu, Bingfeng; Wang, Chenglong

    2016-06-01

    Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is considered to be a major etiological factor for dental caries. In this study, plaques from dental enamel surfaces of caries-active and caries-free individuals were obtained and cultivated for S. mutans isolation. Morphology examination, biochemical characterization, and polymerase chain reaction were performed to identify S. mutans The cariogenicity of S. mutans strains isolated from clinical specimens was evaluated by testing the acidogenicity, aciduricity, extracellular polysaccharide production, and adhesion ability of the bacteria. Finally, subtractive SELEX (systematic evolution of ligands by exponential enrichment) technology targeting whole intact cells was used to screen for ssDNA aptamers specific to the strains with high cariogenicity. After nine rounds of subtractive SELEX, sufficient pool enrichment was achieved as shown by radioactive isotope analysis. The enriched pool was cloned and sequenced randomly, followed by MEME online and RNA structure software analysis of the sequences. Results from the flow cytometry indicated that aptamers H1, H16, H4, L1, L10, and H19 could discriminate highly cariogenic S. mutans strains from poorly cariogenic strains. Among these, Aptamer H19 had the strongest binding capacity with cariogenic S. mutans strains with a dissociation constant of 69.45 ± 38.53 nM. In conclusion, ssDNA aptamers specific to highly cariogenic clinical S. mutans strains were successfully obtained. These ssDNA aptamers might be used for the early diagnosis and treatment of dental caries. © The Author 2016. Published by Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  7. Pathogenic Potential of Two Sibling Species, Anisakis simplex (s.s. and Anisakis pegreffii (Nematoda: Anisakidae: In Vitro and In Vivo Studies

    Directory of Open Access Journals (Sweden)

    Chan-Hyeok Jeon

    2015-01-01

    Full Text Available The pathogenic potentials of two sibling nematodes Anisakis simplex sensu stricto (s.s. and A. pegreffii were compared by in vitro and in vivo studies. Live third-stage larvae of each species were subjected to agar blocks made using PBS or RPMI-1640, overlaid with different supernatants (artificial gastric juice, PBS, and RPMI-1640, and their penetration ability was compared. Their tolerance of artificial gastric juice was also tested. Further, they were introduced into rats by gastric intubation, and the in vivo locations of them were investigated. A. pegreffii showed higher penetration ability than A. simplex (s.s. in most of the experimental conditions, except for the RPMI-1640 agar block overlaid with artificial gastric juice. In an acid tolerance test, the mean survival times were 6.1 days for A. simplex (s.s. and 4.2 days for A. pegreffii. In an animal experiment, A. simplex (s.s. stayed for a shorter time in the stomachs of rats than A. pegreffii. Some A. pegreffii and A. simplex (s.s. were embedded in the gastric mucosa or freely existed in the abdominal cavity. All of these results suggest that A. pegreffii has the pathogenic potential to cause anisakidosis in humans when ingested, as does A. simplex (s.s..

  8. Ferulic Acid, But Not All Hydroxycinnamic Acids, Is a Novel T3SS Inducer of Ralstonia solanacearum and Promotes Its Infection Process in Host Plants under Hydroponic Condition

    Directory of Open Access Journals (Sweden)

    Yong Zhang

    2017-09-01

    Full Text Available Hydroxycinnamic acids (HCAs are typical monocyclic phenylpropanoids, including cinnamic acid (Cin, coumaric acid (Cou, caffeic acid (Caf, ferulic acid (FA and their isomers, and involved in the interactions between pathogens and host plants. Here, we focused on the impact of HCAs on expression of type III secretion system (T3SS in Ralstonia solanacearum. FA significantly induced the expression of the T3SS and some type III effectors (T3Es genes in hrp-inducing medium, while did not the other HCAs. However, exogenously supplemented FA did not affect the T3SS expression in planta and the elicitation of the hypersensitive response (HR in tobacco leaves. Consistent with its central roles in pathogenicity, the FA-induced expression of the T3SS led to significant promotion on infection process of R. solanacearum in tomato plants under hydroponics cultivation. Moreover, the FA-induced expression of the T3SS was specifically mediated by the well-characterized signaling cascade PrhA-prhI/R-PrhJ-HrpG-HrpB, independent of the other known regulatory pathways. In summary, our results demonstrated that FA, a novel inducer of the T3SS in R. solanacearum, was able to promote its infection process in host plants under hydroponics condition.

  9. An atypical forkhead-containing transcription factor SsFKH1 is involved in sclerotial formation and is essential for pathogenicity in Sclerotinia sclerotiorum.

    Science.gov (United States)

    Fan, Huidong; Yu, Gang; Liu, Yanzhi; Zhang, Xianghui; Liu, Jinliang; Zhang, Yanhua; Rollins, Jeffrey A; Sun, Fengjie; Pan, Hongyu

    2017-09-01

    Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic plant pathogen with a worldwide distribution. The sclerotia of S. sclerotiorum are pigmented multicellular structures formed from the aggregation of vegetative hyphae. These survival structures play a central role in the life and infection cycles of this pathogen. Here, we characterized an atypical forkhead (FKH)-box-containing protein, SsFKH1, involved in sclerotial development and virulence. To investigate the role of SsFkh1 in S. sclerotiorum, the partial sequence of SsFkh1 was cloned and RNA interference (RNAi)-based gene silencing was employed to alter the expression of SsFkh1. RNA-silenced mutants with significantly reduced SsFkh1 RNA levels exhibited slow hyphal growth and sclerotial developmental defects. In addition, the expression levels of a set of putative melanin biosynthesis-related laccase genes and a polyketide synthase-encoding gene were significantly down-regulated in silenced strains. Disease assays demonstrated that pathogenicity in RNAi-silenced strains was significantly compromised with the development of a smaller infection lesion on tomato leaves. Collectively, the results suggest that SsFkh1 is involved in hyphal growth, virulence and sclerotial formation in S. sclerotiorum. © 2016 BSPP AND JOHN WILEY & SONS LTD.

  10. Characterisation of Shigella Spa33 and Thermotoga FliM/N reveals a new model for C-ring assembly in T3SS.

    Science.gov (United States)

    McDowell, Melanie A; Marcoux, Julien; McVicker, Gareth; Johnson, Steven; Fong, Yu Hang; Stevens, Rebecca; Bowman, Lesley A H; Degiacomi, Matteo T; Yan, Jun; Wise, Adam; Friede, Miriam E; Benesch, Justin L P; Deane, Janet E; Tang, Christoph M; Robinson, Carol V; Lea, Susan M

    2016-02-01

    Flagellar type III secretion systems (T3SS) contain an essential cytoplasmic-ring (C-ring) largely composed of two proteins FliM and FliN, whereas an analogous substructure for the closely related non-flagellar (NF) T3SS has not been observed in situ. We show that the spa33 gene encoding the putative NF-T3SS C-ring component in Shigella flexneri is alternatively translated to produce both full-length (Spa33-FL) and a short variant (Spa33-C), with both required for secretion. They associate in a 1:2 complex (Spa33-FL/C2) that further oligomerises into elongated arrays in vitro. The structure of Spa33-C2 and identification of an unexpected intramolecular pseudodimer in Spa33-FL reveal a molecular model for their higher order assembly within NF-T3SS. Spa33-FL and Spa33-C are identified as functional counterparts of a FliM-FliN fusion and free FliN respectively. Furthermore, we show that Thermotoga maritima FliM and FliN form a 1:3 complex structurally equivalent to Spa33-FL/C2 , allowing us to propose a unified model for C-ring assembly by NF-T3SS and flagellar-T3SS. © 2015 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

  11. Characterisation of Shigella Spa33 and Thermotoga FliM/N reveals a new model for C‐ring assembly in T3SS

    Science.gov (United States)

    McDowell, Melanie A.; Marcoux, Julien; McVicker, Gareth; Johnson, Steven; Fong, Yu Hang; Stevens, Rebecca; Bowman, Lesley A. H.; Degiacomi, Matteo T.; Yan, Jun; Wise, Adam; Friede, Miriam E.; Benesch, Justin L. P.; Deane, Janet E.; Tang, Christoph M.; Robinson, Carol V.

    2015-01-01

    Summary Flagellar type III secretion systems (T3SS) contain an essential cytoplasmic‐ring (C‐ring) largely composed of two proteins FliM and FliN, whereas an analogous substructure for the closely related non‐flagellar (NF) T3SS has not been observed in situ. We show that the spa33 gene encoding the putative NF‐T3SS C‐ring component in S higella flexneri is alternatively translated to produce both full‐length (Spa33‐FL) and a short variant (Spa33‐C), with both required for secretion. They associate in a 1:2 complex (Spa33‐FL/C2) that further oligomerises into elongated arrays in vitro. The structure of Spa33‐C 2 and identification of an unexpected intramolecular pseudodimer in Spa33‐FL reveal a molecular model for their higher order assembly within NF‐T3SS. Spa33‐FL and Spa33‐C are identified as functional counterparts of a FliM–FliN fusion and free FliN respectively. Furthermore, we show that T hermotoga maritima  FliM and FliN form a 1:3 complex structurally equivalent to Spa33‐FL/C2, allowing us to propose a unified model for C‐ring assembly by NF‐T3SS and flagellar‐T3SS. PMID:26538516

  12. Pathogenic Potential of Two Sibling Species, Anisakis simplex (s.s.) and Anisakis pegreffii (Nematoda: Anisakidae): In Vitro and In Vivo Studies

    Science.gov (United States)

    Jeon, Chan-Hyeok; Kim, Jeong-Ho

    2015-01-01

    The pathogenic potentials of two sibling nematodes Anisakis simplex sensu stricto (s.s.) and A. pegreffii were compared by in vitro and in vivo studies. Live third-stage larvae of each species were subjected to agar blocks made using PBS or RPMI-1640, overlaid with different supernatants (artificial gastric juice, PBS, and RPMI-1640), and their penetration ability was compared. Their tolerance of artificial gastric juice was also tested. Further, they were introduced into rats by gastric intubation, and the in vivo locations of them were investigated. A. pegreffii showed higher penetration ability than A. simplex (s.s.) in most of the experimental conditions, except for the RPMI-1640 agar block overlaid with artificial gastric juice. In an acid tolerance test, the mean survival times were 6.1 days for A. simplex (s.s.) and 4.2 days for A. pegreffii. In an animal experiment, A. simplex (s.s.) stayed for a shorter time in the stomachs of rats than A. pegreffii. Some A. pegreffii and A. simplex (s.s.) were embedded in the gastric mucosa or freely existed in the abdominal cavity. All of these results suggest that A. pegreffii has the pathogenic potential to cause anisakidosis in humans when ingested, as does A. simplex (s.s.). PMID:25685821

  13. Corrosion behavior of mild steel and SS 304L in presence of dissolved nickel under aerated and deaerated conditions

    OpenAIRE

    Mohd Mobin; Hina Shabnam

    2011-01-01

    In dual purpose water/power co-generation plants, the presence of high concentration of Cu and Ni in the re-circulating brine/condensate as a result of condenser tubes corrosion has been attributed as one of the several causes of corrosion damage of flash chamber materials and water touched parts of the boilers. The present investigation deals with the effect of dissolved nickel in the concentration range of 10 ppb to 100 ppm on the corrosion behavior of mild steel and SS 304L in two aqueous ...

  14. Polymorphisms of KiSS-1 and GPR54 genes and their relationships with litter size in sheep.

    Science.gov (United States)

    Chu, Mingxing; Xiao, Chaoting; Feng, Tao; Fu, Yan; Cao, Guiling; Fang, Li; Di, Ran; Tang, Qianqian; Huang, Dongwei; Ma, Yuehui; Li, Kui; Li, Ning

    2012-03-01

    The KiSS-1 and GPR54 genes were studied as candidate genes for the prolificacy in sheep. Four pairs of primers were designed to detect single nucleotide polymorphisms of exon 1 of KiSS-1 gene and exon 1, exon 2 and partial exon 5 of GPR54 gene in high fecundity breeds (Small Tail Han and Hu sheep) and low fecundity breeds (Dorset, Texel and Corriedale sheep) by PCR-SSCP. Polymorphisms in exon 1 of KiSS-1 gene were detected in prolific Small Tail Han sheep (AA, AB and BB genotypes) and Hu sheep (AA and CC genotypes), no polymorphism was found in low fecundity sheep breeds (only AA genotype). Polymorphisms in exon 2 of GPR54 gene were detected in prolific Hu sheep (DD and EE genotypes) and no polymorphism was found in prolific Small Tail Han sheep and low fecundity sheep breeds (only DD genotype). No polymorphism was detected in exon 1 and partial exon 5 of GPR54 gene in five sheep breeds. The polymorphic genotypes were sequenced. While compared the BB genotype with the AA genotype, one nucleotide mutation (G1035A) was detected, which resulted in amino acid change, Val25Met. Five nucleotide mutations were detected from AA to CC genotype (C981T, C996T, T997C, C1034G, C1039T), and among them four caused amino acid changes, that is, Arg7Trp, Phe12Leu, Asn24Lys, Ala26Val. While compared the EE genotype with the DD genotype, two nucleotide mutations (T2360C, A2411C) were detected, which gave rise to amino acid changes, Met90Thr and Asp107Ala, respectively. Genotype frequencies of AA, BB and AB were 0.62, 0.05 and 0.33 in Small Tail Han sheep, respectively. The Small Tail Han sheep ewes with genotype BB or AB had 0.88 (P 0.05) lambs more than those with genotype AB. These results preliminarily indicated that the KiSS-1 gene may have some association with prolificacy in sheep.

  15. Evaluation of the influence of electric nets on the behaviour of oviposition site seeking Anopheles gambiae s.s.

    Science.gov (United States)

    Dugassa, Sisay; Lindh, Jenny M; Torr, Steve J; Lindsay, Steven W; Fillinger, Ulrike

    2014-06-19

    Electric nets (e-nets) are used to analyse the flight behaviour of insects and have been used extensively to study the host-oriented flight of tsetse flies. Recently we adapted this tool to analyse the oviposition behaviour of gravid malaria vectors, Anopheles gambiae s.s., orienting towards aquatic habitats and traps by surrounding an artificial pond with e-nets and collecting electrocuted mosquitoes on sticky boards on the ground next to the nets. Here we study whether e-nets themselves affect the responses of gravid An. gambiae s.s.. Dual-choice experiments were carried out in 80 m2 screened semi-field systems where 200 gravid An. gambiae s.s. were released each night for 12 nights per experiment. The numbers of mosquito landing on or approaching an oviposition site were studied by adding detergent to the water in an artificial pond or surrounding the pond with a square of e-nets. We also assessed whether the supporting framework of the nets or the sticky boards used to retain electrocuted mosquitoes influenced the catch. Two similar detergent treated ponds presented in choice tests caught an equal proportion of the mosquitoes released, whereas a pond surrounded by e-nets caught a higher proportion than an open pond (odds ratio (OR) 1.7, 95% confidence interval (CI) 1.1 - 2.7; pelectric nets and the yellow boards on the approach of gravid females towards a pond suggests that the tower-like construction of the square of electric nets did not restrict the approach of females but the yellow sticky boards on the ground attract gravid females to a source of water (OR 2.7 95% CI 1.7 - 4.3; pelectric nets is increased when large yellow sticky boards are placed on the ground next to the e-nets to collect electrocuted mosquitoes, possibly because of increased visual contrast to the aquatic habitat. It is therefore important when comparing two treatments that the same trapping device is used in both. The importance of contrast around artificial habitats might be exploited

  16. Harjoitus- ja hengenpelastusoppaan tekeminen Oulujärven Laivaosakeyhtiön alukseen S/S Kouta

    OpenAIRE

    Heikkinen, Pekka

    2013-01-01

    Opinnäytetyön tarkoituksena oli harjoitus- ja hengenpelastusoppaan laatiminen Oulujärven Laiva-osakeyhtiön alukseen S/S Kouta. Kansainvälisen SOLAS-yleissopimuksen mukaan harjoitusopas voi olla pelastautumisharjoitus-, palontorjuntaharjoitusopas tai niiden molempien yhdistelmä. Kotimaan liikenteessä liikennealueella I toimivalla alle 300 GRT aluksella ei kansallisten määräysten mukaan tarvitse olla SOLAS-yleissopimuksen mukaista harjoitusopasta, hengenpelastuslaitteiden käyttöohjeiden löytymi...

  17. Hoitohenkilökunnan kokemuksia nuorten aikuisten hoitotyön haasteista päihdehoitotyössä

    OpenAIRE

    Tampio, Aliisa; Jääskeläinen, Minna

    2013-01-01

    Tutkimuksen tarkoituksena oli selvittää sairaanhoitajien kokemuksia nuorten aikuisten hoitotyön ongelmista päihdehoitotyössä. Tutkimuksen Tavoitteena oli tuottaa tietoa päihdenuorten hoitotyön toteutumisesta sekä mahdollisista haasteista hoitotyöhön liittyen. Oleellista tutkimuksessa oli tuoda hoitajien omia kokemuksia esille. Tutkimus toteutettiin puolistrukturoiduilla kyselylomakkeilla. Tutkimuksen kohderyhmä koostui sairaanhoitajista, jotka työskentelivät Kanta-Hämeessä päihteitä käytt...

  18. Sähköenergian varastointitekniikat älykkäässä sähköverkossa

    OpenAIRE

    Seppänen, Juha

    2014-01-01

    Sähkön varastoinnilla on tulevaisuuden sähköverkoissa tärkeä tehtävä, kun uusiutuvien energianlähteiden ja hajautetun tuotannon määrät kasvavat nykyisestä huomattavasti. Jotta asetetut tavoitteet ekologisuudelle, energiatehokkuudelle ja sähköverkon toimivuudelle täyttyisivät, ne vaatisivat energiavarastojen hyödyntämistä. Energiavarastojen kokonaiskustannusten täytyy kuitenkin olla kilpailukykyisiä muiden vaihtoehtojen rinnalla, jotta niitä käytettäisiin laajasti. Insinöörityössä selvitet...

  19. Role of T3SS-1 SipD Protein in Protecting Mice against Non-typhoidal Salmonella Typhimurium.

    Directory of Open Access Journals (Sweden)

    Bakhos Jneid

    2016-12-01

    Full Text Available Salmonella enterica species are enteric pathogens that cause severe diseases ranging from self-limiting gastroenteritis to enteric fever and sepsis in humans. These infectious diseases are still the major cause of morbidity and mortality in low-income countries, especially in children younger than 5 years and immunocompromised adults. Vaccines targeting typhoidal diseases are already marketed, but none protect against non-typhoidal Salmonella. The existence of multiple non-typhoidal Salmonella serotypes as well as emerging antibiotic resistance highlight the need for development of a broad-spectrum protective vaccine. All Salmonella spp. utilize two type III Secretion Systems (T3SS 1 and 2 to initiate infection, allow replication in phagocytic cells and induce systemic disease. T3SS-1, which is essential to invade epithelial cells and cross the barrier, forms an extracellular needle and syringe necessary to inject effector proteins into the host cell. PrgI and SipD form, respectively, the T3SS-1 needle and the tip complex at the top of the needle. Because they are common and highly conserved in all virulent Salmonella spp., they might be ideal candidate antigens for a subunit-based, broad-spectrum vaccine.We investigated the immunogenicity and protective efficacy of PrgI and SipD administered by subcutaneous, intranasal and oral routes, alone or combined, in a mouse model of Salmonella intestinal challenge. Robust IgG (in all immunization routes and IgA (in intranasal and oral immunization routes antibody responses were induced against both proteins, particularly SipD. Mice orally immunized with SipD alone or SipD combined with PrgI were protected against lethal intestinal challenge with Salmonella Typhimurium (100 Lethal Dose 50% depending on antigen, route and adjuvant.Salmonella T3SS SipD is a promising antigen for the development of a protective Salmonella vaccine, and could be developed for vaccination in tropical endemic areas to control

  20. Taidelähtöiset menetelmät lasten hoitotyössä : Hoitonetti

    OpenAIRE

    Kyrölä, Laura; Riikonen, Anni-Veera

    2013-01-01

    Taidelähtöiset menetelmät lasten hoitotyössä toimivat suojaavina, vapauttavina ja välittävinä asioina, joiden avulla saattaa tulla esille jotain, jolle lapsi ei ole aikaisemmin löytänyt sanoja. Lapsen muistia saattaa aktivoida fyysinen tekeminen eri tavalla kuin puhuminen. Kun lapsi kykenee kertomaan omasta ongelmastaan hänelle mieluisalla tavalla, kokemuksesta tulee merkityksellinen ja lapsi tuntee, että häntä kuunnellaan. Taide tarjoaa lapselle mahdollisuuden tehdä valintoja ja antaa tunte...