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Sample records for prunus serotina black

  1. Agrobacterium-medicated transformation of mature Prunus serotina (black cherry) and regeneration of trangenic shoots

    Science.gov (United States)

    Xiaomei Liu; Paula Pijut

    2010-01-01

    A protocol for Agrobacterium-mediated transformation was developed for in vitro leaf explants of an elite, mature Prunus serotina tree. Agrobacterium tumefaciens strain EHA105 harboring an RNAi plasmid with the black cherry AGAMOUS (AG) gene was used. Bacteria were induced...

  2. Cloning and characterization of prunus serotina AGAMOUS, a putative flower homeotic gene

    Science.gov (United States)

    Xiaomei Liu; Joseph Anderson; Paula Pijut

    2010-01-01

    Members of the AGAMOUS subfamily of MADS-box transcription factors play an important role in regulating the development of reproductive organs in flowering plants. To help understand the mechanism of floral development in black cherry (Prunus serotina), PsAG (a putative flower homeotic identity gene) was isolated...

  3. Early growth performances of various seed sources of black (Prunus ...

    African Journals Online (AJOL)

    Early growth performances of various seed sources of black (Prunus serotina Erhr.) and wild cherry ( Prunus avium L.) seedlings on low and high elevation sites in the western Black Sea Region of Turkey.

  4. Can Prunus serotina be genetically engineered for reproductive sterility and insect pest resistance?

    Science.gov (United States)

    Ying Wang; Paula M. Pijut

    2014-01-01

    Black cherry (Prunus serotina) is a valuable hardwood timber species, and its value highly depends on the wood quality which is often threatened by insect pests. Transgenic black cherry plants that are more resistant to cambial-mining insects may reduce the occurrence of gummosis and have great economic benefits to landowners and the forest products...

  5. Nutraceutical Value of Black Cherry Prunus serotina Ehrh. Fruits: Antioxidant and Antihypertensive Properties

    Directory of Open Access Journals (Sweden)

    Francisco J. Luna-Vázquez

    2013-11-01

    Full Text Available In Mexico black cherry (Prunus serotina Ehrh. fruits are consumed fresh, dried or prepared in jam. Considering the evidence that has linked intake of fruits and vegetables rich in polyphenols to cardiovascular risk reduction, the aim of this study was to characterize the phenolic profile of black cherry fruits and to determine their antioxidant, vasorelaxant and antihypertensive effects. The proximate composition and mineral contents of these fruits were also assessed. Black cherry fruits possess a high content of phenolic compounds and display a significant antioxidant capacity. High-performance liquid chromatography/mass spectrometric analysis indicated that hyperoside, anthocyanins and chlorogenic acid were the main phenolic compounds found in these fruits. The black cherry aqueous extract elicited a concentration-dependent relaxation of aortic rings and induced a significant reduction on systolic blood pressure in L-NAME induced hypertensive rats after four weeks of treatment. Proximate analysis showed that black cherry fruits have high sugar, protein, and potassium contents. The results derived from this study indicate that black cherry fruits contain phenolic compounds which elicit significant antioxidant and antihypertensive effects. These findings suggest that these fruits might be considered as functional foods useful for the prevention and treatment of cardiovascular diseases.

  6. Crystal Macropattern Development in Prunus serotina (Rosaceae, Prunoideae) Leaves

    OpenAIRE

    LERSTEN, NELS R.; HORNER, HARRY T.

    2006-01-01

    • Background and Aims Prunus, subgenus Padus, exhibits two completely different calcium oxalate crystal macropatterns in mature leaves. Foliar macropattern development has been described previously in P. virginiana, representing one version. Prunus serotina, in the group exhibiting the second macropattern, is described here. The goal was to describe developmental details for comparison with P. virginiana, and to extend the sparse current knowledge of crystal macropatterns.

  7. Physiological and foliar symptom response of Prunus serotina, Fraxinus americana and Acer rubrum canopy trees to ozone under differing site conditions

    Science.gov (United States)

    M. Schaub; J.M. Skelly; J.W. Zhang; J.A. Ferdinand; J.E. Savage; R.E. Stevenson; D.D. Davis; K.C. Steiner

    2005-01-01

    The crowns of five canopy dominant black cherry ( Prunus serotina Ehrh.), five white ash ( Fraxinus americana L.), and six red maple ( Acer rubrum L.) trees on naturally differing environmental conditions were accessed with scaffold towers within a mixed hardwood forest stand in central Pennsylvania....

  8. PHYSIOLOGICAL AND FOLIAR INJURY RESPONSES OF PRUNUS SEROTINA, FRAXINUS AMERICANA, AND ACER RUBRUM SEEDLINGS TO VARYING SOIL MOISTURE AND OZONE. (R825244)

    Science.gov (United States)

    Sixteen black cherry (Prunus serotina, Ehrh.), 10 white ash (Fraxinus americana, L.) and 10 red maple (Acer rubrum, L.) 1-year old seedlings were planted per plot in 1997 on a former nursery bed within 12 open-top chambers and six open plots. Seedlings wer...

  9. Risico voor fruitbomen en inheemse bomen na bestrijding van Amerikaanse vogelkers (Prunus serotina) met loodglansschimmel (Chondrostereum purpureum) = [Risk to fruit trees and native trees due to control of black cherry (Prunus serotina) by silverleaf fungus (Chondrostereum purpureum)

    NARCIS (Netherlands)

    Jong, de M.D.

    1988-01-01

    The shrub Prunus serotina , introduced from North America, became a forest pest in the Netherlands. Biological control was considered using the fungus Chondrostereum purpureum , commonly present as a saprophyte and parasite in wood. C. purpureum can cause

  10. Soil feedback and pathogen activity in Prunus serotina throughout its native range

    NARCIS (Netherlands)

    Reinhart, K.O.; Royo, A.A.; Putten, van der W.H.; Clay, K.

    2005-01-01

    1 Oomycete soil pathogens are known to have a negative effect on Prunus serotina seedling establishment and to promote tree diversity in a deciduous forest in Indiana, USA. Here, we investigate whether negative feedbacks operate widely in its native range in eastern USA. 2 In laboratory experiments,

  11. Soil feedback and pathogen activity in Prunus serotina throughout its native range

    Science.gov (United States)

    Kurt O. Reinhart; Alejandro Royo; Wim H. Van der Putten; Keith Clay

    2005-01-01

    1 Oomycete soil pathogens are known to have a negative effect on Prunus serotina seedling establishment and to promote tree diversity in a deciduous forest in Indiana, USA. Here, we investigate whether negative feedbacks operate widely in its native range in eastern USA. 2 In laboratory experiments, soil sterilization was used to test the...

  12. Antioxidant and antimicrobial activity of capulin (Prunus serotina subsp capuli) extracts

    OpenAIRE

    Jimenez, M.; Castillo, I.; Azuara, E.; Beristain, C.I.

    2011-01-01

    Capulin (Prunus serotina subsp. capuli) is an annual fruit widely used in Mexico for the elaboration of several traditional products, such as medicinal tea, which is considered to present antioxidant and antimicrobial properties. The aim of this work was to evaluate the antioxidant and antimicrobial properties of aqueous, acetone, ethanol and methanol extracts. The ethanol extract presented a high anthocyanin (102±7.70 mg Cyd-3-glu/100 g extract) and polyphenol (1732±43.40 mg GAE /100 g extra...

  13. Leaf age affects the responses of foliar injury and gas exchange to tropospheric ozone in Prunus serotina seedlings

    Science.gov (United States)

    Jianwei Zhang; Marcus Schaub; Jonathan A. Ferdinand; John M. Skelly; Kim C. Steiner; James E. Savage

    2010-01-01

    We investigated the effect of leaf age on the response of net photosynthesis (A), stomatal conductance (gwv), foliar injury, and leaf nitrogen concentration (NL) to tropospheric ozone (O3) on Prunus serotina seedlings grown in open-plots (AA) and open-top...

  14. Crystal macropattern development in Prunus serotina (Rosaceae, Prunoideae) leaves.

    Science.gov (United States)

    Lersten, Nels R; Horner, Harry T

    2006-05-01

    Prunus, subgenus Padus, exhibits two completely different calcium oxalate crystal macropatterns in mature leaves. Foliar macropattern development has been described previously in P. virginiana, representing one version. Prunus serotina, in the group exhibiting the second macropattern, is described here. The goal was to describe developmental details for comparison with P. virginiana, and to extend the sparse current knowledge of crystal macropatterns. Leaves at various developmental stages were removed from local trees and from herbarium specimens. Early leaf stages and freehand leaf and stem sections were mounted directly in aqueous glycerine; larger leaves were processed whole or in representative pieces in household bleach, dehydrated in alcohol/xylol, and mounted in Permount. Crystals were detected microscopically between crossed polarizers. Bud scales have a dense druse population. Druses appear first at the stipule tip and proliferate basipetally but soon stop forming; growing stipules therefore have a declining density of druses. Druses appear at the tip of leaves virginiana, and shows that two closely related species can develop radically different modes of crystallization. The few detailed macropattern studies to date reveal striking variations that indicate a new level of organization that must be integrated with the anatomical, physiological and molecular approaches that have been dominant so far.

  15. Evaluation of ozone injury on foliage of black cherry (Prunus serotina) and tall milkweed (Asclepias exaltata) in Great Smoky Mountains National Park.

    Science.gov (United States)

    Chappelka, A; Renfro, J; Somers, G; Nash, B

    1997-01-01

    The incidence and severity of visible foliar ozone injury on black cherry (Prunus serotina) seedlings and saplings and tall milkweed (Asclepias exaltata) plants in Great Smoky Mountains National Park (GRSM) were determined by surveys along selected trails conducted during late summer 1992. The incidence (% injured plants) of ozone injury on black cherry was 47% and the percent injured leaves/injured plant and average leaf area injured were 43 and 6%, respectively. Maximum severity (avg. leaf area of the most severely injured leaf) was 12%. Black cherry seedlings and saplings exhibiting ozone injury were taller than non-injured plants. When insect feeding was present, it occurred 96% of the time on plants with ozone injury. Significantly more injury (p=0.007) on black cherry (% injured leaves/injured black cherry) occurred in the NW section of GRSM compared with the other Park sections. Regression analyses showed no relationships in ozone injury with respect to aspect, slope or elevation. Tall milkweed was evaluated twice during August for ozone injury. The incidence (% injured plants) of ozone injury was 74 and 79% for the first and second survey, respectively. The percentage of injured leaves per plant from the first to second survey was 63 to 79%, respectively. Tall milkweeds showing ozone injury were taller than the non-injured plants. The percentage of insect-damaged plants was 50% among plants without ozone injury and 60% among ozone-injured plants. Non-injured tall milkweed had fewer flowers and/or pods than the injured plants. Mean leaf area injured increased over time, and mean maximum leaf area injured increased from 8 to 11% during the same period. Regression analyses showed no differences in ozone injury regarding aspect, slope or elevation. Our findings indicate that ozone injury is widespread throughout the Park on sensitive vegetation.

  16. Leaf area and net photosynthesis during development of Prunus serotina seedlings

    Science.gov (United States)

    Stephen B. Horsley; Kurt W. Gottschalk

    1993-01-01

    We used the plastochron index to study the relationship between plant age, leaf age and development, and net photosynthesis of black cherry (Prtmus serotina Ehrh.) seedlings. Leaf area and net photosynthesis were measured on all leaves >=75 mm of plants ranging in age from 7 to 20 plastochrons. Effects of plant developmental stage...

  17. Adaptability of black walnut, black cherry, and Northern red oak to Northern California

    Science.gov (United States)

    Philip M. McDonald

    1987-01-01

    When planted in sheltered sites in northern California, only 49% of black walnut (Juglans nigra L.) and 58% of black cherry (Prunus serotina Ehrh.) survived for 15 years, and 20% of northern red oak (Quercus rubra L.) survived for 10 years. The black walnut trees averaged 0.6 inches diameter at breast...

  18. Simultaneous quantification by HPLC of the phenolic compounds for the crude drug of Prunus serotina subsp. capuli.

    Science.gov (United States)

    Rivero-Cruz, Blanca

    2014-08-01

    Prunus serotina Ehrenb. subsp. capuli (Cav.) McVaugh (Rosaceae), commonly known as "capulin", is a native North American tree, commercialized and used in folk medicine for the treatment of the hypertension, gastrointestinal illnesses, and cough. This work developed a suitable HPLC method for quantifying the major active constituents of the infusion of P. serotina, the most important preparation consumed by populations around the world. The analytical method was performed using a Fortis-RP column (150 mm × 4.6 mm; film thickness 5 µm). The mobile phase consisted of an isocratic acetate buffer solution (pH 2.7; A) and methanol (B) (65:35 v/v) at a flow rate of 1.0 mL min(-1). The proposed method was applied to the quantification of 1-3 in several samples of the leaves of P. serotina. The results indicated that amounts of 1-3 in the samples analyzed are uniform, and greater amounts of chlorogenic acid (2; 479.9 ± 33.6 µg g(-1), dry matter) along with hyperoside (1; 185.7 ± 55.3 µg g(-1), dry matter) were present. On the other hand, benzaldehyde (3; 118.2 ± 12.1 µg g(-1) dry matter) was found to be in lower concentration. A simple, sensitive, precise, and reproducible HPLC method for the simultaneous quantification of 1-3 in P. serotina was developed and validated. This is the first report on the quantification of 1-3 as active principles, and compound 1 was selected as a marker of P. serotina, which could be useful to guarantee the quality of the crude drug and herbal products.

  19. Ozone uptake (flux) as it relates to ozone-induced foliar symptoms of Prunus serotina and Populus maximowizii x trichocarpa

    International Nuclear Information System (INIS)

    Orendovici-Best, T.; Skelly, J.M.; Davis, D.D.; Ferdinand, J.A.; Savage, J.E.; Stevenson, R.E.

    2008-01-01

    Field studies were conducted during 2003 and 2004 from early June to the end of August, at 20 sites of lower or higher elevation within north-central Pennsylvania, using seedlings of black cherry (Prunus serotina, Ehrh.) and ramets of hybrid poplar (Populus maximowizii x trichocarpa). A linear model was developed to estimate the influence of local environmental conditions on stomatal conductance. The most significant factors explaining stomatal variance were tree species, air temperature, leaf vapor pressure deficit, elevation, and time of day. Overall, environmental factors explained less than 35% of the variation in stomatal conductance. Ozone did not affect gas exchange rates in either poplar or cherry. Ozone-induced foliar injury was positively correlated with cumulative ozone exposures, expressed as SUM40. Overall, the amount of foliar injury was better correlated to a flux-based approach rather than to an exposure-based approach. More severe foliar injuries were observed on plants growing at higher elevations. - Within heterogeneous environments, ozone flux does not completely explain the variation observed in ozone-induced visible injury

  20. Leaf age affects the responses of foliar injury and gas exchange to tropospheric ozone in Prunus serotina seedlings

    International Nuclear Information System (INIS)

    Zhang Jianwei; Schaub, Marcus; Ferdinand, Jonathan A.; Skelly, John M.; Steiner, Kim C.; Savage, James E.

    2010-01-01

    We investigated the effect of leaf age on the response of net photosynthesis (A), stomatal conductance (g wv ), foliar injury, and leaf nitrogen concentration (N L ) to tropospheric ozone (O 3 ) on Prunus serotina seedlings grown in open-plots (AA) and open-top chambers, supplied with either carbon-filtered or non-filtered air. We found significant variation in A, g wv , foliar injury, and N L (P 3 treatments. Seedlings in AA showed the highest A and g wv due to relatively low vapor pressure deficit (VPD). Older leaves showed significantly lower A, g wv , N L , and higher foliar injury (P wv , and foliar injury to O 3 . Both VPD and N L had a strong influence on leaf gas exchange. Foliar O 3 -induced injury appeared when cumulative O 3 uptake reached 8-12 mmol m -2 , depending on soil water availability. The mechanistic assessment of O 3 -induced injury is a valuable approach for a biologically relevant O 3 risk assessment for forest trees. - Ozone effects on symptom development and leaf gas exchange interacted with leaf age and N-content on black cherry seedlings.

  1. Light energy partitioning, photosynthetic efficiency and biomass allocation in invasive Prunus serotina and native Quercus petraea in relation to light environment, competition and allelopathy.

    Science.gov (United States)

    Robakowski, Piotr; Bielinis, Ernest; Sendall, Kerrie

    2018-05-01

    This study addressed whether competition under different light environments was reflected by changes in leaf absorbed light energy partitioning, photosynthetic efficiency, relative growth rate and biomass allocation in invasive and native competitors. Additionally, a potential allelopathic effect of mulching with invasive Prunus serotina leaves on native Quercus petraea growth and photosynthesis was tested. The effect of light environment on leaf absorbed light energy partitioning and photosynthetic characteristics was more pronounced than the effects of interspecific competition and allelopathy. The quantum yield of PSII of invasive P. serotina increased in the presence of a competitor, indicating a higher plasticity in energy partitioning for the invasive over the native Q. petraea, giving it a competitive advantage. The most striking difference between the two study species was the higher crown-level net CO 2 assimilation rates (A crown ) of P. serotina compared with Q. petraea. At the juvenile life stage, higher relative growth rate and higher biomass allocation to foliage allowed P. serotina to absorb and use light energy for photosynthesis more efficiently than Q. petraea. Species-specific strategies of growth, biomass allocation, light energy partitioning and photosynthetic efficiency varied with the light environment and gave an advantage to the invader over its native competitor in competition for light. However, higher biomass allocation to roots in Q. petraea allows for greater belowground competition for water and nutrients as compared to P. serotina. This niche differentiation may compensate for the lower aboveground competitiveness of the native species and explain its ability to co-occur with the invasive competitor in natural forest settings.

  2. Leaf age affects the responses of foliar injury and gas exchange to tropospheric ozone in Prunus serotina seedlings

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Jianwei, E-mail: jianweizhang@fs.fed.u [Environmental Resources Research Institute, Pennsylvania State University, University Park, PA 16802 (United States); School of Forest Resources, Pennsylvania State University, University Park, PA 16802 (United States); Schaub, Marcus; Ferdinand, Jonathan A. [Environmental Resources Research Institute, Pennsylvania State University, University Park, PA 16802 (United States); Skelly, John M. [Department of Plant Pathology, Pennsylvania State University, University Park, PA 16802 (United States); Steiner, Kim C. [School of Forest Resources, Pennsylvania State University, University Park, PA 16802 (United States); Savage, James E. [Department of Plant Pathology, Pennsylvania State University, University Park, PA 16802 (United States)

    2010-08-15

    We investigated the effect of leaf age on the response of net photosynthesis (A), stomatal conductance (g{sub wv}), foliar injury, and leaf nitrogen concentration (N{sub L}) to tropospheric ozone (O{sub 3}) on Prunus serotina seedlings grown in open-plots (AA) and open-top chambers, supplied with either carbon-filtered or non-filtered air. We found significant variation in A, g{sub wv}, foliar injury, and N{sub L} (P < 0.05) among O{sub 3} treatments. Seedlings in AA showed the highest A and g{sub wv} due to relatively low vapor pressure deficit (VPD). Older leaves showed significantly lower A, g{sub wv}, N{sub L}, and higher foliar injury (P < 0.001) than younger leaves. Leaf age affected the response of A, g{sub wv}, and foliar injury to O{sub 3}. Both VPD and N{sub L} had a strong influence on leaf gas exchange. Foliar O{sub 3}-induced injury appeared when cumulative O{sub 3} uptake reached 8-12 mmol m{sup -2}, depending on soil water availability. The mechanistic assessment of O{sub 3}-induced injury is a valuable approach for a biologically relevant O{sub 3} risk assessment for forest trees. - Ozone effects on symptom development and leaf gas exchange interacted with leaf age and N-content on black cherry seedlings.

  3. Occurrence of gum spots in black cherry after partial harvest cutting

    Science.gov (United States)

    Charles O. Rexrode; H. Clay Smith; H. Clay Smith

    1990-01-01

    Bark beetles, primarily the bark beetle Phlosotribus liminori (Harris), are the major cause of gum spots in sawtimber-size black cherry Prunus serotina Ehrh. Approximately 90 percent of all gum spots in the bole sections are caused by bark beetles. Gum spots were studied in 95 black cherry trees near Parsons, West Virginia. Over 50 percent of the bark beetle-caused gum...

  4. Ozone exposure, uptake, and response of different-sized black cherry trees

    Science.gov (United States)

    Todd S. Frederickson; John M. Skelly; Kim C. Steiner; Thomas E. Kolb

    1996-01-01

    Differences in exposure, uptake and relative sensitivity to ozone between seedling, sapling, and canopy black cherry (Prunus serotina Ehrh.) trees were characterized during two growing seasons in north central Pennsylvania. Open-grown trees of all sizes received a similar amount of ozone exposure. Seedlings had greater foliar ozone injury, expressed...

  5. Acoustic tomography for decay detection in black cherry trees

    Science.gov (United States)

    Xiping Wang; Jan Wiedenbeck; Shanqing Liang

    2009-01-01

    This study investigated the potential of using acoustic tomography for detecting internal decay in high-value hardwood trees in the forest. Twelve black cherry (Prunus serotina) trees that had a wide range of physical characteristics were tested in a stand of second-growth hardwoods in Kane, PA, using a PiCUS Sonic Tomograph tool. The trees were felled after the field...

  6. Physiological and foliar injury responses of Prunus serotina, Fraxinus americana, and Acer rubrum seedlings to varying soil moisture and ozone

    International Nuclear Information System (INIS)

    Schaub, M.; Skelly, J.M.; Steiner, K.C.; Davis, D.D.; Pennypacker, S.P.; Zhang, J.; Ferdinand, J.A.; Savage, J.E.; Stevenson, R.E.

    2003-01-01

    High soil water availability favors ozone uptake, increases foliar injury, and exacerbates the negative ozone effect on gas exchange of seedlings of deciduous tree species. - Sixteen black cherry (Prunus serotina, Ehrh.), 10 white ash (Fraxinus americana, L.) and 10 red maple (Acer rubrum, L.) 1-year old seedlings were planted per plot in 1997 on a former nursery bed within 12 open-top chambers and six open plots. Seedlings were exposed to three different ozone scenarios (ambient air: 100% O 3 ; non-filtered air: 98% ambient O 3 ; charcoal-filtered air: 50% ambient O 3 ) within each of two different water regimes (nine plots irrigated, nine plots non-irrigated) during three growing seasons. During the 1998 and 1999 growing season, leaf gas exchange, plant water relations, and foliar injury were measured. Climatic data, ambient- and chamber-ozone-concentrations were monitored. We found that seedlings grown under irrigated conditions had similar (in 1998) but significantly higher gas exchange rates (in 1999) than seedlings grown within non-irrigated plots among similar ozone exposures. Cherry and ash had similar ozone uptake but cherry developed more ozone-induced injury (<34% affected leaf area, LAA) than ash (<5% LAA), while maple rarely showed foliar injury, indicating the species differed in ozone sensitivity. Significantly more severe injury on seedlings grown under irrigated conditions than seedlings grown under non-irrigated conditions demonstrated that soil moisture altered seedling responses to ambient ozone exposures

  7. Validating the Operational Draft Regional Guidebook for the Functional Assessment of High-Gradient Ephemeral and Intermittent Headwater Streams in Western West Virginia and Eastern Kentucky

    Science.gov (United States)

    2014-04-01

    blackgum, wild black cherry (Prunus serotina), sweet cherry (Prunus avium), slippery elm (Ulmus rubra), sourwood (Oxydendrum arboreum), red maple, and...virginiana), American hornbeam (Carpinus caroliniana), and slippery elm . Buffalo nut (Pyrularia pubera), wild hydrangea (Hydrangea arborescens...Pinus strobus), wild black cherry, boxelder (Acer negundo), sycamore, black locust (Robinia pseudo-acacia), slippery elm , and white ash. The shrub

  8. Incorporation of an invasive plant into a native insect herbivore food web

    NARCIS (Netherlands)

    Schilthuizen, Menno; Santos Pimenta, Lúcia P; Lammers, Youri; Steenbergen, Peter J; Flohil, Marco; Beveridge, Nils G P; van Duijn, Pieter T; Meulblok, Marjolein M; Sosef, Nils; van de Ven, Robin; Werring, Ralf; Beentjes, Kevin K; Meijer, Kim; Vos, Rutger A; Vrieling, Klaas; Gravendeel, Barbara; Choi, Young; Verpoorte, Robert; Smit, Chris; Beukeboom, Leo W

    2016-01-01

    The integration of invasive species into native food webs represent multifarious dynamics of ecological and evolutionary processes. We document incorporation of Prunus serotina (black cherry) into native insect food webs. We find that P. serotina harbours a herbivore community less dense but more

  9. Physiological and foliar symptom response in the crowns of Prunus serotina, Fraxinus americana and Acer rubrum canopy trees to ambient ozone under forest conditions

    International Nuclear Information System (INIS)

    Schaub, M.; Skelly, J.M.; Zhang, J.W.; Ferdinand, J.A.; Savage, J.E.; Stevenson, R.E.; Davis, D.D.; Steiner, K.C.

    2005-01-01

    The crowns of five canopy dominant black cherry (Prunus serotina Ehrh.), five white ash (Fraxinus americana L.), and six red maple (Acer rubrum L.) trees on naturally differing environmental conditions were accessed with scaffold towers within a mixed hardwood forest stand in central Pennsylvania. Ambient ozone concentrations, meteorological parameters, leaf gas exchange and leaf water potential were measured at the sites during the growing seasons of 1998 and 1999. Visible ozone-induced foliar injury was assessed on leaves within the upper and lower crown branches of each tree. Ambient ozone exposures were sufficient to induce typical symptoms on cherry (0-5% total affected leaf area, LAA), whereas foliar injury was not observed on ash or maple. There was a positive correlation between increasing cumulative ozone uptake (U) and increasing percent of LAA for cherry grown under drier site conditions. The lower crown leaves of cherry showed more severe foliar injury than the upper crown leaves. No significant differences in predawn leaf water potential (ψ L ) were detected for all three species indicating no differing soil moisture conditions across the sites. Significant variation in stomatal conductance for water vapor (g wv ) was found among species, soil moisture, time of day and sample date. When comparing cumulative ozone uptake and decreased photosynthetic activity (P n ), red maple was the only species to show higher gas exchange under mesic vs. drier soil conditions (P wv and P n demonstrate the strong influence of heterogeneous environmental conditions within forest canopies. - Within the heterogeneous environment of a mature forest, many factors in addition to soil moisture play a significant role in determining exposure/response relationships to ozone

  10. Polyphyly of the Padus group of Prunus (Rosaceae) and the evolution of biogeographic disjunctions between eastern Asia and eastern North America.

    Science.gov (United States)

    Liu, Xiao-Lin; Wen, Jun; Nie, Ze-Long; Johnson, Gabriel; Liang, Zong-Suo; Chang, Zhao-Yang

    2013-05-01

    Prunus subgenus Padus is a group with a wide distribution in temperate eastern Asia and eastern North America with one species extending to Europe and one to Central America. Phylogenetic relationships of subgenus Padus were reconstructed using sequences of nuclear ribosomal ITS, and plastid ndhF gene, and rps16 intron and rpl16 intron. Prunus subgenus Padus is shown to be polyphyletic. Taxa of subgenus Padus and subgenus Laurocerasus are highly intermixed in both the ITS and the plastid trees. The results support two disjunctions between eastern North America and Eurasia within the Padus group. One disjunction is between Prunus virginiana of eastern North America and P. padus of Eurasia, estimated to have diverged at 2.99 (95 % HPD 0.59-6.15)-4.1 (95 % HPD 0.63-8.59) mya. The other disjunction is between P. serotina and its Asian relatives. The second disjunction may have occurred earlier than the former one, but the age estimate is difficult due to the unresolved phylogenetic position of the P. serotina complex.

  11. National Wetland Plant List Indicator Rating Definitions

    Science.gov (United States)

    2012-07-01

    glaucus (blue rye grass), Eragrostis pilosa (Indian love grass), Oenothera biennis (king’s-cureall), Ostrya virginiana (eastern hop-hornbeam...profile with a low wa- ter-holding capacity. ERDC/CRREL TN-12-1 6 Prunus serotina (black cherry), Phleum pretense (common timothy), Sarcobatus

  12. Environmental Impact Research Program. Eastern Gray Squirrel (Sciurus carolinensis). Section 4.7.1, US Army Corps of Engineers Wildlife Resources Management Manual.

    Science.gov (United States)

    1986-07-01

    Palmetto Sabal spp. Pawpaw Asimina triloba Pecan Carya illinoensis Persimmon Diospyros virginiana Pine Pinus app. Loblolly pine P. taeda Red mulberry...grandifotia Bitter pecan Carya aquatica Blackberry Rubus app. Black cherry Prunus serotina Blackgum Nyssa sylvatica Black walnut Jugtans nigra Blueberry...americana Hickory Carya Spp. Bitternut hickory C. cordiformis Shagbark hickory C. ovata Shellbark hickory C. Zaciniosa Hophornbeam Ostrya virginiana

  13. Hanseniaspora nodinigri, a new yeast species found in black knots (Dibotryon morbosum) of Prunus virginiana.

    Science.gov (United States)

    Lachance, M A

    1981-07-01

    The new yeast species Hanseniaspora nodinigri is described to accommodate members of the genus Hanseniaspora that are unable to assimilate glucono-sigma-lactone and isolated from stromatal tissue of black knots (Dobotryon morbosum) of chokecherry, Prunus virginiana. The newly described taxon shows much resemblance, by other criteria, to H. vineae van der Walt et Tscheuschner and H. osmophila (Niehaus) Phaff, Miller et Shifrine.

  14. Xylem development in prunus flower buds and the relationship to deep supercooling.

    Science.gov (United States)

    Ashworth, E N

    1984-04-01

    Xylem development in eight Prunus species was examined and the relationship to deep supercooling assessed. Dormant buds of six species, P. armeniaca, P. avium, P. cerasus, P. persica, P. salicina, and P. sargentii deep supercooled. Xylem vessel elements were not observed within the dormant floral primordia of these species. Instead, discrete bundles containing procambial cells were observed. Vascular differentiation resumed and xylem continuity was established during the time that the capacity to deep supercool was lost. In P. serotina and P. virginiana, two species which do not supercool, xylem vessels ran the length of the inflorescence and presumably provided a conduit for the spread of ice into the bud. The results support the hypothesis that the lack of xylem continuity is an important feature of buds which deep supercool.

  15. Prunus fruit juices

    NARCIS (Netherlands)

    Toydemir, Gamze; Boyacioglu, Dilek; Hall, R.D.; Beekwilder, M.J.; Capanoglu, Esra

    2017-01-01

    The juice drinks obtained from Prunus fruit species, apricot (Prunus armeniaca), cherry (sweet cherry (Prunus avium) and sour cherry (Prunus cerasus)), peach (Prunus persica), and plum (Prunus domestica), are gaining increasing interest as a convenient alternative to fresh fruits. The conventional

  16. Characterization of a new (R)-hydroxynitrile lyase from the Japanese apricot Prunus mume and cDNA cloning and secretory expression of one of the isozymes in Pichia pastoris.

    Science.gov (United States)

    Fukuta, Yasuhisa; Nanda, Samik; Kato, Yasuo; Yurimoto, Hiroya; Sakai, Yasuyoshi; Komeda, Hidenobu; Asano, Yasuhisa

    2011-01-01

    PmHNL, a hydroxynitrile lyase from Japanese apricot ume (Prunus mume) seed was purified to homogeneity by ammonium sulfate fractionation and chromatographic steps. The purified enzyme was a monomer with molecular mass of 58 kDa. It was a flavoprotein similar to other hydroxynitrile lyases of the Rosaceae family. It was active over a broad temperature, and pH range. The N-terminal amino acid sequence (20 amino acids) was identical with that of the enzyme from almond (Prunus dulcis). Based on the N-terminal sequence of the purified enzyme and the conserved amino acid sequences of the enzymes from Pr. dulcis, inverse PCR method was used for cloning of a putative PmHNL (PmHNL2) gene from a Pr. mume seedling. Then the cDNA for the enzyme was cloned. The deduced amino acid sequence was found to be highly similar (95%) to that of an enzyme from Pr. serotina, isozyme 2. The recombinant Pichia pastoris transformed with the PmHNL2 gene secreted an active enzyme in glycosylated form.

  17. 7 CFR 301.74-2 - Regulated articles.

    Science.gov (United States)

    2010-01-01

    ... following are regulated articles: (a) All plant material and plant parts of Prunus (stone fruit) species.... serotina, P. serrula, P. serrulata, P. subhirtella, P. yedoensis, and P. virginiana, except for seeds and...

  18. Isolation and structure elucidation of some compounds of Solidago gigantea var. serotina (O. Kuntze) Cronquist

    International Nuclear Information System (INIS)

    Lichtenberger, E.

    1985-03-01

    Studies on plants of the genus Solidago used in folk medicine as a remedy against diseases of the urinary-system induced the investigation of Solidago gigantea Aiton var. serotina (O. Kuntze) Cronquist = S. serotina. A methanolic extract of the aerial parts was examined for the biologically active saponin complex. The most effective constituents were found to be a mixture of closely related triterpene glycosides named saponin G, H, I and saponin K. Fractionation over a series of silicagel columns and droplet countercurrent chromatographies resulted in pure saponin G and saponin H. The screening of the hydrolized glycosides afforded the genins bayogenin as major compound, oleanolic acid and three additional aglycones in trace quantities. The identy of bayogenin, oleanolic acid and bayogeninglucosid was established by mass-, 1 H-NMR- and 13 C-NMR-measurements and by direct comparison with authentic samples. Gas chromatographic analysis of the sugar moiety yielded rhamnose, xylose, glucose and 6-desoxyglucose after KILIANI-degradation in a ratio 3:3:1:1. Beside the triterpene derivatives, mentioned above, we found a diterpene, sterol and sterolglycosed as well. The diterpene structure was confirmed by UV-, IR-, mass-, 1 H-NMR- and 13 C-NMR-spectroscopy. This diterpene was not previously known to be present in S. serotina. The sterol-compounds could be determined as α-spinasterol and the respective hexosid. (Author)

  19. Survey of Prunus necrotic ringspot virus in Rose and Its Variability in Rose and Prunus spp.

    Science.gov (United States)

    Moury, B; Cardin, L; Onesto, J P; Candresse, T; Poupet, A

    2001-01-01

    ABSTRACT A survey for viruses in rose propagated in Europe resulted in detection of only Prunus necrotic ringspot virus (PNRSV) among seven viruses screened. Four percent of cut-flower roses from different sources were infected with PNRSV. Progression of the disease under greenhouse conditions was very slow, which should make this virus easy to eradicate through sanitary selection. Comparison of the partial coat protein gene sequences for three representative rose isolates indicated that they do not form a distinct phylogenetic group and show close relations to Prunus spp. isolates. However, a comparison of the reactivity of monoclonal antibodies raised against these isolates showed that the most prevalent PNRSV serotype in rose was different from the most prevalent serotype in Prunus spp. All of the 27 rose isolates tested infected P. persica seedlings, whereas three of the four PNRSV isolates tested from Prunus spp. were poorly infectious in Rosa indica plants. These data suggest adaptation of PNRSV isolates from Prunus spp., but not from rose, to their host plants. The test methodologies developed here to evaluate PNRSV pathogenicity in Prunus spp. and rose could also help to screen for resistant genotypes.

  20. DIAGNOSTICS OF VIRUS PHYTOPATHOGENS FRUIT TREE PLUM POX VIRUS, PRUNUS NECROTIC RINGSPOT VIRUS AND PRUNUS DWARF VIRUS BY BIOLOGICAL AND MOLECULAR DIAGNOSTICS

    Directory of Open Access Journals (Sweden)

    Július Rozák

    2013-02-01

    Full Text Available The aim of this study was to determine the incidence of viral phytopathogen Plum pox virus, Prunus necrotic ringspot virus and Prunus dwarf virus in selected localities of Slovakia and diagnose them using a molecular and biological methods. Forty samples of fruit trees of the genus Prunus, twenty samples from intensive plantings and twenty samples from wild subject were analysed. Biological diagnostic by using biological indicators Prunus persica cv. GF 305, Prunus serrulata cv. Schirofugen and molecular diagnostic by mRT-PCR were applied. Five samples with Plum pox virus were infected. The two samples positive for Prunus necrotic ringspot virus and one sample for Prunus dwarf virus were confirmed. The two samples were found to be infected with two viruses Prunus necrotic ringspot virus and Prunus dwarf virus. This work focuses on two techniques, their application to the diagnosis of stone fruit viruses and their routinely used for sanitary and certification programmes.

  1. Field responses of Prunus serotina and Asclepias syriaca to ozone around southern Lake Michigan.

    Science.gov (United States)

    Bennett, J P; Jepsen, E A; Roth, J A

    2006-07-01

    Higher ozone concentrations east of southern Lake Michigan compared to west of the lake were used to test hypotheses about injury and growth effects on two plant species. We measured approximately 1000 black cherry trees and over 3000 milkweed stems from 1999 to 2001 for this purpose. Black cherry branch elongation and milkweed growth and pod formation were significantly higher west of Lake Michigan while ozone injury was greater east of Lake Michigan. Using classification and regression tree (CART) analyses we determined that departures from normal precipitation, soil nitrogen and ozone exposure/peak hourly concentrations were the most important variables affecting cherry branch elongation, and milkweed stem height and pod formation. The effects of ozone were not consistently comparable with the effects of soil nutrients, weather, insect or disease injury, and depended on species. Ozone SUM06 exposures greater than 13 ppm-h decreased cherry branch elongation 18%; peak 1-h exposures greater than 93 ppb reduced milkweed stem height 13%; and peak 1-h concentrations greater than 98 ppb reduced pod formation 11% in milkweed.

  2. Korte mededeling

    NARCIS (Netherlands)

    Reijerse, Fons (A.) I.

    1997-01-01

    Tijdens de inventarisatie van km-hok 52.14.42 (N. van Venray) trof ik op 3 september 1996 een exemplaar aan van Galeopsis ladanum L. (Brede raai). De plant stond op de overgang van een geploegde akker naar een bosrand (merendeels Pinus sylvestris met ondergroei van Prunus serotina) met

  3. DIAGNOSTICS OF VIRUS PHYTOPATHOGENS FRUIT TREE PLUM POX VIRUS, PRUNUS NECROTIC RINGSPOT VIRUS AND PRUNUS DWARF VIRUS BY BIOLOGICAL AND MOLECULAR DIAGNOSTICS

    OpenAIRE

    Július Rozák; Zdenka Gálová

    2013-01-01

    The aim of this study was to determine the incidence of viral phytopathogen Plum pox virus, Prunus necrotic ringspot virus and Prunus dwarf virus in selected localities of Slovakia and diagnose them using a molecular and biological methods. Forty samples of fruit trees of the genus Prunus, twenty samples from intensive plantings and twenty samples from wild subject were analysed. Biological diagnostic by using biological indicators Prunus persica cv. GF 305, Prunus serrulata cv. Schirofugen a...

  4. Field responses of Prunus serotina and Asclepias syriaca to ozone around southern Lake Michigan

    Energy Technology Data Exchange (ETDEWEB)

    Bennett, J.P. [U.S. Geological Survey and Institute for Environmental Studies, University of Wisconsin, Madison, WI 53706 (United States)]. E-mail: jpbennet@wisc.edu; Jepsen, E.A. [Bureau of Air Management, Wisconsin Department of Natural Resources, Madison, WI 53707 (United States); Roth, J.A. [Bureau of Air Management, Wisconsin Department of Natural Resources, Madison, WI 53707 (United States)

    2006-07-15

    Higher ozone concentrations east of southern Lake Michigan compared to west of the lake were used to test hypotheses about injury and growth effects on two plant species. We measured approximately 1000 black cherry trees and over 3000 milkweed stems from 1999 to 2001 for this purpose. Black cherry branch elongation and milkweed growth and pod formation were significantly higher west of Lake Michigan while ozone injury was greater east of Lake Michigan. Using classification and regression tree (CART) analyses we determined that departures from normal precipitation, soil nitrogen and ozone exposure/peak hourly concentrations were the most important variables affecting cherry branch elongation, and milkweed stem height and pod formation. The effects of ozone were not consistently comparable with the effects of soil nutrients, weather, insect or disease injury, and depended on species. Ozone SUM06 exposures greater than 13 ppm-h decreased cherry branch elongation 18%; peak 1-h exposures greater than 93 ppb reduced milkweed stem height 13%; and peak 1-h concentrations greater than 98 ppb reduced pod formation 11% in milkweed. - Decreased cherry branch elongation, milkweed stem height and pod production, and foliar injury on both species occurred at sites around southern Lake Michigan at ozone exposures of 13 SUM06 ppm-h and 93-98 ppb peak hourly.

  5. Field responses of Prunus serotina and Asclepias syriaca to ozone around southern Lake Michigan

    International Nuclear Information System (INIS)

    Bennett, J.P.; Jepsen, E.A.; Roth, J.A.

    2006-01-01

    Higher ozone concentrations east of southern Lake Michigan compared to west of the lake were used to test hypotheses about injury and growth effects on two plant species. We measured approximately 1000 black cherry trees and over 3000 milkweed stems from 1999 to 2001 for this purpose. Black cherry branch elongation and milkweed growth and pod formation were significantly higher west of Lake Michigan while ozone injury was greater east of Lake Michigan. Using classification and regression tree (CART) analyses we determined that departures from normal precipitation, soil nitrogen and ozone exposure/peak hourly concentrations were the most important variables affecting cherry branch elongation, and milkweed stem height and pod formation. The effects of ozone were not consistently comparable with the effects of soil nutrients, weather, insect or disease injury, and depended on species. Ozone SUM06 exposures greater than 13 ppm-h decreased cherry branch elongation 18%; peak 1-h exposures greater than 93 ppb reduced milkweed stem height 13%; and peak 1-h concentrations greater than 98 ppb reduced pod formation 11% in milkweed. - Decreased cherry branch elongation, milkweed stem height and pod production, and foliar injury on both species occurred at sites around southern Lake Michigan at ozone exposures of 13 SUM06 ppm-h and 93-98 ppb peak hourly

  6. A Regional Guidebook for Conducting Functional Assessments of Wetland and Riparian Forests in the Ouachita Mountains and Crowley’s Ridge Regions of Arkansas

    Science.gov (United States)

    2006-12-01

    Liquidambar styraciflua Gleditsia triacanthos Quercus alba Pinus taeda Juniperus virginiana Quercus pagoda Quercus nigra Prunus serotina Quercus phellos...maple, sugarberry (Celtis laevigata), American elm (Ulmus americana), and persimmon (Diospyros virginiana ). Wetlands of higher terraces typically are...hazels (Hamamelis virginiana , H. vernalis), and highbush blueberry (Vaccinium arboreum). The groundcover layer is usually very diverse and may include

  7. Micropropagation of ornamental Prunus spp. and GF305 peach, a Prunus viral indicator.

    Science.gov (United States)

    Kalinina, Anna; Brown, Daniel C W

    2007-07-01

    A micropropagation approach was developed for nine ornamental Prunus species, P. americana, P. cistena, P. glandulosa, P. serrulata 'Kwanzan', P. laurocerasus, P. sargentii, P. tomentosa, P. triloba, P. virginiana 'Schubert', commercially important in North America, and GF305 peach, commonly used for Prunus virus indexing. The micropropagation cycle based on proliferation of vegetative tissues includes establishment of tissue culture through introduction of shoot meristems in vitro, shoot proliferation, root induction and plant acclimatization steps and can be completed in 5 months. A meristem sterilization protocol minimized bacterial and fungal contamination. Multiple shoot formation in ornamental Prunus was obtained through the use of 1 mg l(-1) 6-benzyladenine. For GF305 peach, alteration in the sugar composition, fructose instead of sucrose, and addition of 1 mg l(-1 )ferulic acid had a significant impact on the shoot proliferation rate and maintenance of long-term in vitro culture. Rooting and plant acclimatization conditions were improved using a two-step protocol with a 4-day root induction in indole-3-butiric acid (IBA)-containing media with consequent 3-week root elongation in IBA-free media. One-month incubation of rooted shoots in a vermiculite-based medium resulted in additional shoot and root growth and provided better acclimatization and plant recovery. The micropropagation approach can be used for maintenance of the clonal properties for Prunus spp. as well as a protocol to support meristem therapy against viral infection.

  8. Draft Environmental Impact Statement. Space Shuttle Advanced Solid Rocket Motor Program

    Science.gov (United States)

    1988-12-01

    species, including orchids ( Orchidaceae ) and several carnivorous plants, such as sundews (Drosera sp.), pitcher plants (Sarracenia sp.) and pipeworts...a dense shrub cover including saw palmetto, sea grape (Coccoloba urifera), wax myrtle (Myrica cerifera), and tough buckthorn (Bumelia tenax) (George...Cherry Prunus serotina Shrubs Galberries Ilex glabra, I. coriacea Wax Myrtle yrica cerifeFa Vines Bamboo Vine Similax laurifolia Poison Ivy Rhus radicans

  9. Community development on 35-year-old planted minespoil banks in Pennsylvania

    Energy Technology Data Exchange (ETDEWEB)

    Schuster, W S; Hutnik, R J

    1987-07-01

    Invading plant communities were studied in 1982 on a series of 35-year old strip-mine test plantings in the main bituminous region of Pennsylvania. Ten of the original 22 sites were evaluated; the others had been significantly disturbed or destroyed. The sites varied in mean pH, in survival and basal area of the planted trees and in the species planted. The study involved 24 plots of the following species: eastern white pine (Pine strobus), red pine (Pinus resinosa), Japanese larch (Laprix leptolepis), red oak (Quercus rubra), black locust (Robinia pseudoacacia), white ash (Fraxinus americana), green ash (Fraxinus pennsylvanica) and hybrid poplar (Populus hyb.) and seven plots where plantings were unsuccessful (control plots). In the cluster analysis of invading communities described by a modified Braun-Blanquet scale, strong clustering trends were evident by both site and planted species. Clustering by sites was most pronounced for those sites with extremes of soil pH. There was also strong clustering among pine plots and among some hardwood plots. Species richness was higher on white ash, red oak, Japanese larch and control plots than on white pine plots. Black cherry (Prunus serotina) was the most common of the 169 invading species and was especially numerous on black locust plots. Aspens (Populus sp.) were also common invaders, especially on pine and ash plots. These species, along with pin cherry (Prunus Pennsylvanica) and the planted black locust are declining in importance, whereas black cherry, red maple (Acer rubrum) and slippery elm (Ulmus rubra) are increasing. Invading oaks (Quercus sp.), hickories (Carya sp.) and a few sugar maple (Acer saccharum) and American beech (Fagus grandifolia) suggest future successional changes. 26 refs., 2 figs., 4 tabs.

  10. A molecular phylogeny of selected species of genus Prunus L ...

    African Journals Online (AJOL)

    (Syn. Prunus amygdalus) and Prunus cornuta (Wall. ex. Royle) Steudel. These are indigenous to Pakistan. In the ITS strict consensus results for example, the clade consisting of Laurocerasus, Padus and Cerasus subgenera are sister to the rest of the clades in the phylogenetic tree. Key words: Phylogeny, Prunus, Pakistan, ...

  11. Manejo y síndromes de domesticación del capulín (Prunus serotina Ehrh ssp. capuli (Cav.) Mc Vaugh) en comunidades del estado de Tlaxcala

    OpenAIRE

    Avendaño-Gómez, Aidé; Lira-Saade, Rafael; Madrigal-Calle, Beatriz; García-Moya, Edmundo; Soto-Hernández, Marcos; Romo de Vivar-Romo, Alfonso

    2015-01-01

    Existen alrededor de 5000 a 7000 especies de plantas en México en estados evolutivos tempranos e intermedios hasta llegar a los domesticados y un ejemplo es el capulín (Prunus serótina Ehrh ssp. capuli (Cav.) Mc Vaugh) clasificado como domesticado, pero sin estudios sobre los mecanismos de selección e intervención que lo llevaron a este grado de manejo. Por lo tanto, el objetivo de este estudio fue describir aspectos etnobotánicos relacionados con el uso, reconocimiento de la variación y el m...

  12. Prunus dulcis, Batch

    African Journals Online (AJOL)

    STORAGESEVER

    2010-06-07

    Jun 7, 2010 ... almond (Prunus dulcis, Batch) genotypes as revealed by PCR analysis. Yavar Sharafi1*, Jafar Hajilou1, Seyed AbolGhasem Mohammadi2, Mohammad Reza Dadpour1 and Sadollah Eskandari3. 1Department of Horticulture, Faculty of Agriculture, University of Tabriz, Tabriz, 5166614766, Iran.

  13. Development and cross-species/genera transferability of microsatellite markers discovered using 454 genome sequencing in chokecherry (Prunus virginiana L.).

    Science.gov (United States)

    Wang, Hongxia; Walla, James A; Zhong, Shaobin; Huang, Danqiong; Dai, Wenhao

    2012-11-01

    Chokecherry (Prunus virginiana L.) (2n = 4x = 32) is a unique Prunus species for both genetics and disease-resistance research due to its tetraploid nature and X-disease resistance. However, no genetic and genomic information on chokecherry is available. A partial chokecherry genome was sequenced using Roche 454 sequencing technology. A total of 145,094 reads covering 4.8 Mbp of the chokecherry genome were generated and 15,113 contigs were assembled, of which 11,675 contigs were larger than 100 bp in size. A total of 481 SSR loci were identified from 234 (out of 11,675) contigs and 246 polymerase chain reaction (PCR) primer pairs were designed. Of 246 primers, 212 (86.2 %) effectively produced amplification from the genomic DNA of chokecherry. All 212 amplifiable chokecherry primers were used to amplify genomic DNA from 11 other rosaceous species (sour cherry, sweet cherry, black cherry, peach, apricot, plum, apple, crabapple, pear, juneberry, and raspberry). Thus, chokecherry SSR primers can be transferable across Prunus species and other rosaceous species. An average of 63.2 and 58.7 % of amplifiable chokecherry primers amplified DNA from cherry and other Prunus species, respectively, while 47.2 % of amplifiable chokecherry primers amplified DNA from other rosaceous species. Using random genome sequence data generated from next-generation sequencing technology to identify microsatellite loci appears to be rapid and cost-efficient, particularly for species with no sequence information available. Sequence information and confirmed transferability of the identified chokecherry SSRs among species will be valuable for genetic research in Prunus and other rosaceous species. Key message A total of 246 SSR primers were identified from chokecherry genome sequences. Of which, 212 were confirmed amplifiable both in chokecherry and other 11 other rosaceous species.

  14. Use of bioindicators and passive sampling devices to evaluate ambient ozone concentrations in north central Pennsylvania

    Energy Technology Data Exchange (ETDEWEB)

    Yuska, D.E.; Skelly, J.M.; Ferdinand, J.A.; Stevenson, R.E.; Savage, J.E.; Mulik, J.D.; Hines, A

    2003-09-01

    Passive samplers and bioindicator plants detect ozone air pollution in north central Pennsylvania. - Ambient concentrations of tropospheric ozone and ozone-induced injury to black cherry (Prunus serotina) and common milkweed (Asclepias syriaca) were determined in north central Pennsylvania from 29 May to 5 September 2000 and from 28 May to 18 September 2001. Ogawa passive ozone samplers were utilized within openings at 15 forested sites of which six were co-located with TECO model 49 continuous ozone monitors. A significant positive correlation was observed between the Ogawa passive samplers and the TECO model 49 continuous ozone monitors for the 2000 (r=0.959) and 2001 (r=0.979) seasons. In addition, a significant positive correlation existed in 2000 and 2001 between ozone concentration and elevation (r=0.720) and (r=0.802), respectively. Classic ozone-induced symptoms were observed on black cherry and common milkweed. In 2000, initial injury was observed in early June, whereas for the 2001 season, initial injury was initially observed in late June. During both seasons, injury was noted at most sites by mid- to late-July. Soil moisture potential was measured for the 2001 season and a significant positive relationship (P<0.001) showed that injury to black cherry was a function of cumulative ozone concentrations and available soil moisture.

  15. Use of bioindicators and passive sampling devices to evaluate ambient ozone concentrations in north central Pennsylvania

    International Nuclear Information System (INIS)

    Yuska, D.E.; Skelly, J.M.; Ferdinand, J.A.; Stevenson, R.E.; Savage, J.E.; Mulik, J.D.; Hines, A.

    2003-01-01

    Passive samplers and bioindicator plants detect ozone air pollution in north central Pennsylvania. - Ambient concentrations of tropospheric ozone and ozone-induced injury to black cherry (Prunus serotina) and common milkweed (Asclepias syriaca) were determined in north central Pennsylvania from 29 May to 5 September 2000 and from 28 May to 18 September 2001. Ogawa passive ozone samplers were utilized within openings at 15 forested sites of which six were co-located with TECO model 49 continuous ozone monitors. A significant positive correlation was observed between the Ogawa passive samplers and the TECO model 49 continuous ozone monitors for the 2000 (r=0.959) and 2001 (r=0.979) seasons. In addition, a significant positive correlation existed in 2000 and 2001 between ozone concentration and elevation (r=0.720) and (r=0.802), respectively. Classic ozone-induced symptoms were observed on black cherry and common milkweed. In 2000, initial injury was observed in early June, whereas for the 2001 season, initial injury was initially observed in late June. During both seasons, injury was noted at most sites by mid- to late-July. Soil moisture potential was measured for the 2001 season and a significant positive relationship (P<0.001) showed that injury to black cherry was a function of cumulative ozone concentrations and available soil moisture

  16. Two Types of New Natural Materials for Fruit Vinegar in Prunus Plants

    Directory of Open Access Journals (Sweden)

    Zhao Han

    2017-01-01

    Full Text Available To increase Prunus armeniaca × P. sibirica and P. domestica × P. armeniaca added value; three natural fruit vinegars were designed. The results showed the nutrition of Prunus domestica × P. armeniaca cultivar Fengweimeigui vinegar (T1 had high minerals and microelements, especially the Ca and Mg reached to the 150.00mg/L, 85.40 mg/L, respectively; the vinegar of Prunus armeniaca × P. sibirica cultivar Zhongren No.1 (T2 not only have rich Na (2800.00 mg/L, P (123.00 mg/L, but also have plentiful amino acid that content reached to 200.08 mg/L. However, the mixture vinegar (T3 with pulps from Prunus domestica × P. armeniaca and Prunus armeniaca × P. sibirica had the middle nutrient contents, but the property was balanced. We therefore conclude that solid fermentation is a suitable method to preserve nutrients and value-added for Prunus plants fruit, and three types vinegars are suitable for different age people, and the difference nutrient contents and typical characteristic indicate that three vinegars are competitive products in market.

  17. Characterization of cytokinin signaling and homeostasis gene families in two hardwood tree species: Populus trichocarpa and Prunus persica.

    Science.gov (United States)

    Immanen, Juha; Nieminen, Kaisa; Duchens Silva, Héctor; Rodríguez Rojas, Fernanda; Meisel, Lee A; Silva, Herman; Albert, Victor A; Hvidsten, Torgeir R; Helariutta, Ykä

    2013-12-16

    Through the diversity of cytokinin regulated processes, this phytohormone has a profound impact on plant growth and development. Cytokinin signaling is involved in the control of apical and lateral meristem activity, branching pattern of the shoot, and leaf senescence. These processes influence several traits, including the stem diameter, shoot architecture, and perennial life cycle, which define the development of woody plants. To facilitate research about the role of cytokinin in regulation of woody plant development, we have identified genes associated with cytokinin signaling and homeostasis pathways from two hardwood tree species. Taking advantage of the sequenced black cottonwood (Populus trichocarpa) and peach (Prunus persica) genomes, we have compiled a comprehensive list of genes involved in these pathways. We identified genes belonging to the six families of cytokinin oxidases (CKXs), isopentenyl transferases (IPTs), LONELY GUY genes (LOGs), two-component receptors, histidine containing phosphotransmitters (HPts), and response regulators (RRs). All together 85 Populus and 45 Prunus genes were identified, and compared to their Arabidopsis orthologs through phylogenetic analyses. In general, when compared to Arabidopsis, differences in gene family structure were often seen in only one of the two tree species. However, one class of genes associated with cytokinin signal transduction, the CKI1-like family of two-component histidine kinases, was larger in both Populus and Prunus than in Arabidopsis.

  18. CLONING AND SEQUENCING OF PGIP FROM ‘JIN SERIES’ ALMOND (PRUNUS DULCIS

    Directory of Open Access Journals (Sweden)

    Yuhu Han

    2015-12-01

    Full Text Available Specific primers synthesized according to conservative regions of polygalacturonase inhibiting protein (PGIP gene were used to amplify Prunus Dulcis genomic DNA by polymerase-chain reaction (PCR. Six bands (pgip1, pgip2, pgip3, pgip4, pgip5 and pgip6 of genes were obtained and cloned into PBS-T vector. According to the length of bands, 717bp, 864bp, 796bp were A1 (pgip1, pgip2, pgip3, A2 (pgip4, A4 (pgip5, pgip6, respectively. DNA sequences showed that the fragments taken together were the gene encoding PGIP. A2 and A3 contained two exons interrupted by one intron, which has GT-AG sequence. Its DNA and amino acid sequences were highly homologies to those from Prunus Persica; Prunus Salicina; Prunus Americana; Prunus Mume, respectively. A conserved lencinerial fragment exists in the derived protein sequence.

  19. Spatial heterogeneity in post-dispersal predation on Prunus and Uvularia seeds.

    Science.gov (United States)

    Webb, Sara L; Willson, Mary F

    1985-08-01

    We investigated effects of seed density, distance from parent, and habitat (woods, open field) on post-dispersal predation risk (chiefly by rodents) for seeds of Prunus virginiana (Rosaceae). Additional study of the habitat effect (woods, open field, treefall gap) was made with seeds of Prunus avium (Rosaceae) and Uvularia grandiflora (Liliaceae). Density of Prunus seeds (range 2-40 seeds/group) did not affect predation risk for individual seeds. Distance from parent plants did influence predation risk, which was greatest directly beneath parents. This distance effect primarily comprised a sharp drop in risk within 2 m of parents, a distance too small to generate a "spacing rule" for conspecifics.We found that habitat strongly influenced predation intensity. Rates of removal of Prunus seeds were higher in woods than in open fields, except when overall predation intensity was very low and no pattern could be discerned. Prunus seed removal rates were higher in closed woods than in treefall gaps. Consequently, a Prunus seed will more likely escape predation if dispersed to an open site. In contrast, Uvularia seed removal rates were higher in open fields than in woods but did not differ between closed woods and tree-fall gaps.Predation intensity was spatially patchy between and within experimental arrays, but was consistent over time at some specific points in space, possibly reflecting home ranges of seed predators.

  20. A fruit quality gene map of Prunus

    Directory of Open Access Journals (Sweden)

    Bliss Fredrick A

    2009-12-01

    Full Text Available Abstract Background Prunus fruit development, growth, ripening, and senescence includes major biochemical and sensory changes in texture, color, and flavor. The genetic dissection of these complex processes has important applications in crop improvement, to facilitate maximizing and maintaining stone fruit quality from production and processing through to marketing and consumption. Here we present an integrated fruit quality gene map of Prunus containing 133 genes putatively involved in the determination of fruit texture, pigmentation, flavor, and chilling injury resistance. Results A genetic linkage map of 211 markers was constructed for an intraspecific peach (Prunus persica progeny population, Pop-DG, derived from a canning peach cultivar 'Dr. Davis' and a fresh market cultivar 'Georgia Belle'. The Pop-DG map covered 818 cM of the peach genome and included three morphological markers, 11 ripening candidate genes, 13 cold-responsive genes, 21 novel EST-SSRs from the ChillPeach database, 58 previously reported SSRs, 40 RAFs, 23 SRAPs, 14 IMAs, and 28 accessory markers from candidate gene amplification. The Pop-DG map was co-linear with the Prunus reference T × E map, with 39 SSR markers in common to align the maps. A further 158 markers were bin-mapped to the reference map: 59 ripening candidate genes, 50 cold-responsive genes, and 50 novel EST-SSRs from ChillPeach, with deduced locations in Pop-DG via comparative mapping. Several candidate genes and EST-SSRs co-located with previously reported major trait loci and quantitative trait loci for chilling injury symptoms in Pop-DG. Conclusion The candidate gene approach combined with bin-mapping and availability of a community-recognized reference genetic map provides an efficient means of locating genes of interest in a target genome. We highlight the co-localization of fruit quality candidate genes with previously reported fruit quality QTLs. The fruit quality gene map developed here is a

  1. A molecular phylogeny of selected species of Genus Prunus L ...

    African Journals Online (AJOL)

    Administrator

    2011-05-30

    May 30, 2011 ... The genus Prunus L. is an important plant for fruit production and it includes plums, apricots, cherries, almonds ... classification and placement of different genera under different sub-families. ... cultivated primarily or their beautiful flowers, such as ..... described the character evolution in the 37 Prunus and 8.

  2. Radiosensitivity and recovery of tree crowns in a gamma-irradiated northern forest community

    International Nuclear Information System (INIS)

    Buech, R.R.

    1977-01-01

    Crown mortality was observed on 13 tree species in a gamma-irradiated forest community located near Rhinelander, Wis. Observations at the end of the first (1973) and second (1974) postirradiation growing seasons are presented for each species. Crown mortality was most severe during 1973 for Acer rubrum, A. saccharum, Ostrya virginiana, Populus tremuloides, Prunus serotina, Quercus rubra, and Ulmus americana. For some species, however, crown mortality was most severe during 1974. These were Betula alleghaniensis, B. papyrifera, Tilia americana, and possibly Fraxinus americana and F. nigra. These differences in year of primary response are explained on the basis of bud differentiation and mortality. The most resistant species were A. saccharum, O. virginiana, and Populus tremuloides, and the most sensitive were T. americana and U. americana. Others were intermediate in sensitivity. Taken collectively, exposures less than 2 or 3 kr did not seriously affect crowns of most species, whereas exposures exceeding about 40 kr were lethal to the crowns of all tree species. The observed zonation of tree mortality closely approximated predictions published by others except for underestimating the resistance of A. saccharum and overestimating the resistance of T. americana, F. americana, and Prunus serotina. Not surprisingly, the tree stratum of the northern forest community was found to be more radiosensitive than lichen, grassland, or herbaceous communities in other irradiation studies

  3. Characterization of sour (Prunus cerasus L. and sweet cherry (Prunus avium L. varieties with five isozyme systems Caracterização de variedades de ginjeira (Prunus cerasus L. e cerejeira (Prunus avium L. em cinco sistemas isoenzimáticos

    Directory of Open Access Journals (Sweden)

    Remedios Morales Corts

    2008-03-01

    Full Text Available Extracts from young leaves of nine sweet cherry (Prunus avium L. and eight sour cherry (Prunus cerasus L. varieties, located in the germplasm collection of the 'Direção Regional de Agricultura da Beira Interior' (Fundão, Portugal, were analysed for five isozyme systems in order to characterise these varieties and detect problems of synonymies and homonymies that frequently present. The sweet and sour cherry varieties analyzed showed low isoenzymatic polymorphism, being PGM and PGI the systems with the highest discrimination power. These systems presented seven and five different zymogrames, respectively. IDH showed four patterns. SKDH and 6-PGD grouped the varieties only into two patterns. The evident and discriminant restrictions of this type of analysis had got results that have only been a complement for agronomical and morphological characterization.Nove extratos de folhas com desenvolvimento encompleto foram obtidos de 9 variedades de cerejeira (Prunus avium L. e 8 de ginjeira (Prunus cerasus L., localizados no campo de germoplasma da "Direcção Regional de Agricultura da Beira Interior" (Fundão, Portugal. Esses extratos foram analisados para 5 sistemas isoenzimáticos a fim de caracterizar essas variedades e simultaneamente detectar sinonímias e homonímias, freqüentes neste tipo de estudos. As variedades de cerejeira e ginjeira analisadas mostraram baixo polimorfismo isoenzimático, sendo que os sistemas PGM e PGI mostraram maior poder de discriminação. Estes sistemas apresentaram 7 e 5 zimogramas diferentes, respectivamente. IDH mostrou 4 padrões. SKDH e 6-PGD agruparam as variedades em 2 padrões. Os evidentes limites discriminatórios desse tipo de análise serviram somente de complemento para a caracterização agronômica e morfológica.

  4. Appalachian hardwood stump sprouts are potential sawlog crop trees. Research note NE-299. [Oak, maple, linden, cherry trees

    Energy Technology Data Exchange (ETDEWEB)

    Lanson, N.I.

    1976-01-01

    Dbh and height of 8- and 12-year-old coppice shoots and the number of live shoots were recorded for 736 stumps at least 12 inches in diameter of Liriodendron tulipifera, Prunus serotina, Quercus rubra, Acer rubrum, and Tilia americana in north-central West Virgina. Of the 8-year-old shoots, T. americana had the greatest number of live stems/stump (16.8) and the greatest number of potential crop trees (dominant or co-dominant shoots attached to the stump not more than 6 inches above the ground), averaging 2.5 stems/stump. The average numbers of potential crop trees/stump were similar at 8 and 12 year. At 12 years, at least 88% of stumps of each species had at least one stem of groundline origin with no forks below 25 feet were 84% for L. tulipifera, 59% for Q rubra, 73% for A. rubrum, and 65% for P. Serotina.

  5. Physicochemical characterisation of four cherry species (Prunus spp.) grown in China.

    Science.gov (United States)

    Cao, Jinping; Jiang, Qing; Lin, Juanying; Li, Xian; Sun, Chongde; Chen, Kunsong

    2015-04-15

    The physicochemical characteristics of four cherry species (Prunus avium, Prunus cerasus, Prunus pseudocerasus and Prunus tomentosa) were evaluated. Inter-species variability was greater than intra-species differences. Glucose and fructose were the main sugars, and malic acid was the main organic acid in all species. Combining HPLC-DAD and LC-ESI-MS/MS technologies, total 25 phenolic components were preliminarily identified. P. avium was characterised by high fruit weight, edible proportion, sugar content and low acid content, which made it suitable for fresh eating. P. cerasus was high in acid content and anthocyanins content, making it a good processing species. P. pseudocerasus had rich flavonols varieties and high proportion of hydrocinnamic acids. P. tomentosa was characterised by high total phenolics content (especially flavonols and tannins) and antioxidant activity, indicating a great developmental potential as a health fruit. The results of the present study might provide theoretical guidance for the further development and utilisation of cherries. Copyright © 2014 Elsevier Ltd. All rights reserved.

  6. Genomic segments RNA1 and RNA2 of Prunus necrotic ringspot virus codetermine viral pathogenicity to adapt to alternating natural Prunus hosts.

    Science.gov (United States)

    Cui, Hongguang; Hong, Ni; Wang, Guoping; Wang, Aiming

    2013-05-01

    Prunus necrotic ringspot virus (PNRSV) affects Prunus fruit production worldwide. To date, numerous PNRSV isolates with diverse pathological properties have been documented. To study the pathogenicity of PNRSV, which directly or indirectly determines the economic losses of infected fruit trees, we have recently sequenced the complete genome of peach isolate Pch12 and cherry isolate Chr3, belonging to the pathogenically aggressive PV32 group and mild PV96 group, respectively. Here, we constructed the Chr3- and Pch12-derived full-length cDNA clones that were infectious in the experimental host cucumber and their respective natural Prunus hosts. Pch12-derived clones induced much more severe symptoms than Chr3 in cucumber, and the pathogenicity discrepancy between Chr3 and Pch12 was associated with virus accumulation. By reassortment of genomic segments, swapping of partial genomic segments, and site-directed mutagenesis, we identified the 3' terminal nucleotide sequence (1C region) in RNA1 and amino acid K at residue 279 in RNA2-encoded P2 as the severe virulence determinants in Pch12. Gain-of-function experiments demonstrated that both the 1C region and K279 of Pch12 were required for severe virulence and high levels of viral accumulation. Our results suggest that PNRSV RNA1 and RNA2 codetermine viral pathogenicity to adapt to alternating natural Prunus hosts, likely through mediating viral accumulation.

  7. The genome of Prunus mume.

    Science.gov (United States)

    Zhang, Qixiang; Chen, Wenbin; Sun, Lidan; Zhao, Fangying; Huang, Bangqing; Yang, Weiru; Tao, Ye; Wang, Jia; Yuan, Zhiqiong; Fan, Guangyi; Xing, Zhen; Han, Changlei; Pan, Huitang; Zhong, Xiao; Shi, Wenfang; Liang, Xinming; Du, Dongliang; Sun, Fengming; Xu, Zongda; Hao, Ruijie; Lv, Tian; Lv, Yingmin; Zheng, Zequn; Sun, Ming; Luo, Le; Cai, Ming; Gao, Yike; Wang, Junyi; Yin, Ye; Xu, Xun; Cheng, Tangren; Wang, Jun

    2012-01-01

    Prunus mume (mei), which was domesticated in China more than 3,000 years ago as ornamental plant and fruit, is one of the first genomes among Prunus subfamilies of Rosaceae been sequenced. Here, we assemble a 280M genome by combining 101-fold next-generation sequencing and optical mapping data. We further anchor 83.9% of scaffolds to eight chromosomes with genetic map constructed by restriction-site-associated DNA sequencing. Combining P. mume genome with available data, we succeed in reconstructing nine ancestral chromosomes of Rosaceae family, as well as depicting chromosome fusion, fission and duplication history in three major subfamilies. We sequence the transcriptome of various tissues and perform genome-wide analysis to reveal the characteristics of P. mume, including its regulation of early blooming in endodormancy, immune response against bacterial infection and biosynthesis of flower scent. The P. mume genome sequence adds to our understanding of Rosaceae evolution and provides important data for improvement of fruit trees.

  8. Incidence of ozone symptoms on vegetation within a National Wildlife Refuge in New Jersey, USA

    International Nuclear Information System (INIS)

    Davis, Donald D.; Orendovici, Teodora

    2006-01-01

    During 1993-1996 and 2001-2003, we evaluated the percentage of plants (incidence) exhibiting ozone-induced foliar symptoms on vegetation within a National Wildlife Refuge located along the Atlantic Ocean coast of New Jersey, USA. Incidence varied among plant species and years. Bioindicator plants most sensitive to ozone, across all years, included native common milkweed (Asclepias syriaca) and wild grape (Vitis spp.), as well as introduced tree-of-heaven (Ailanthus altissima). Less sensitive bioindicators included Virginia creeper (Parthenocissus quinquefolia) and winged sumac (Rhus coppolina). Black cherry (Prunus serotina) and sassafras (Sassafras albidum) were least sensitive. The greatest incidence of ozone symptoms, across all plant species, occurred in 1996, followed by 2001 > 1995 > 1994 > 1993 > 2003 > 2002. A model was developed that showed a statistically significant relationship between incidence of ozone symptoms and the following parameters: plant species, Palmer Drought Severity Index, and the interaction of W126 x N100 measures of ambient ozone. - Vegetation in a National Wildlife Refuge containing a Class I wilderness area exhibits foliar symptoms from ambient ozone

  9. Incidence of ozone symptoms on vegetation within a National Wildlife Refuge in New Jersey, USA

    Energy Technology Data Exchange (ETDEWEB)

    Davis, Donald D. [Department of Plant Pathology, Ecology Program, Penn State Institutes of the Environment, Pennsylvania State University, University Park, PA 16803 (United States)]. E-mail: ddd2@psu.edu; Orendovici, Teodora [Department of Plant Pathology, Ecology Program, Penn State Institutes of the Environment, Pennsylvania State University, University Park, PA 16803 (United States)

    2006-10-15

    During 1993-1996 and 2001-2003, we evaluated the percentage of plants (incidence) exhibiting ozone-induced foliar symptoms on vegetation within a National Wildlife Refuge located along the Atlantic Ocean coast of New Jersey, USA. Incidence varied among plant species and years. Bioindicator plants most sensitive to ozone, across all years, included native common milkweed (Asclepias syriaca) and wild grape (Vitis spp.), as well as introduced tree-of-heaven (Ailanthus altissima). Less sensitive bioindicators included Virginia creeper (Parthenocissus quinquefolia) and winged sumac (Rhus coppolina). Black cherry (Prunus serotina) and sassafras (Sassafras albidum) were least sensitive. The greatest incidence of ozone symptoms, across all plant species, occurred in 1996, followed by 2001 > 1995 > 1994 > 1993 > 2003 > 2002. A model was developed that showed a statistically significant relationship between incidence of ozone symptoms and the following parameters: plant species, Palmer Drought Severity Index, and the interaction of W126 x N100 measures of ambient ozone. - Vegetation in a National Wildlife Refuge containing a Class I wilderness area exhibits foliar symptoms from ambient ozone.

  10. Pondering the monoterpene composition of Pinus serotina Michx.: can limonene be used as a chemotaxonomic marker for the identification of old turpentine stumps?

    Science.gov (United States)

    Thomas L. Eberhardt; Jolie M. Mahfouz; Philip M. Sheridan

    2010-01-01

    Wood samples from old turpentine stumps in Virginia were analyzed by GC-MS to determine if the monoterpene compositions could be used for species identification. Given that limonene is reported to be the predominant monoterpene for pond pine (Pinus serotina Michx.), low relative proportions of limonene in these samples appeared to suggest that these...

  11. Self-incompatibility of Prunus tenella and evidence that reproductively isolated species of Prunus have different SFB alleles coupled with an identical S-RNase allele.

    Science.gov (United States)

    Surbanovski, Nada; Tobutt, Kenneth R; Konstantinović, Miroslav; Maksimović, Vesna; Sargent, Daniel J; Stevanović, Vladimir; Bosković, Radovan I

    2007-05-01

    Many species of Prunus display an S-RNase-based gametophytic self-incompatibility (SI), controlled by a single highly polymorphic multigene complex termed the S-locus. This comprises tightly linked stylar- and pollen-expressed genes that determine the specificity of the SI response. We investigated SI of Prunus tenella, a wild species found in small, isolated populations on the Balkan peninsula, initially by pollination experiments and identifying stylar-expressed RNase alleles. Nine P. tenella S-RNase alleles (S(1)-S(9)) were cloned; their sequence analysis showed a very high ratio of non-synonymous to synonymous nucleotide substitutions (K(a)/K(s)) and revealed that S-RNase alleles of P. tenella, unlike those of Prunus dulcis, show positive selection in all regions except the conserved regions and that between C2 and RHV. Remarkably, S(8)-RNase, was found to be identical to S(1)-RNase from Prunus avium, a species that does not interbreed with P. tenella and, except for just one amino acid, to S(11) of P. dulcis. However, the corresponding introns and S-RNase-SFB intergenic regions showed considerable differences. Moreover, protein sequences of the pollen-expressed SFB alleles were not identical, harbouring 12 amino-acid replacements between those of P. tenella SFB(8) and P. avium SFB(1). Implications of this finding for hypotheses about the evolution of new S-specificities are discussed.

  12. Field Assessment of Gopher Tortoise Habitat at Camp Shelby, MS. Phase II: Overstory and Combined Assessments

    Science.gov (United States)

    2010-10-17

    Magnolia virginiana 0 4.5 0 3.3 0 8.4 Nyssa sylvatica 0 6.7 0 8.8 0 2.5 Pinus echinata 0 0.2 1.8 0.7 0 0 Pinus elliotii 0 0 0.9 0 0.7 0 Pinus...palustris 93.0 53.4 71.9 38.2 88.1 73.6 Pinus taeda 0.5 5.3 0.9 8.8 0.7 1.9 Prunus serotina 0 6.7 1.4 1.1 0 0 Quercus spp. (red) 4.9 16.7 16.1 10.6

  13. Pegamento e crescimento inicial de enxertos do pessegueiro 'Aurora-1' em clones de umezeiro (Prunus mume Sieb. et Zucc. e 'Okinawa' [Prunus persica (L. Batsch] propagados por estacas herbáceas Tissue union and initial growth of 'Aurora-1' peach buds on mume clones (Prunus mume Sieb. et Zucc. and 'Okinawa' [Prunus persica (L. Batsch] propagated by herbaceous cuttings

    Directory of Open Access Journals (Sweden)

    Newton Alex Mayer

    2005-04-01

    Full Text Available O presente estudo teve por objetivo avaliar o pegamento e o crescimento inicial de enxertos do pessegueiro 'Aurora-1' em clones de umezeiro (Prunus mume Sieb. et Zucc. e 'Okinawa' [Prunus persica (L. Batsch] propagados por estacas herbáceas. Realizaram-se dois experimentos, adotando-se a enxertia de borbulhia por escudo (março e borbulhia por escudo modificada (julho. Com os resultados obtidos, pode-se concluir que é viável a realização da enxertia do 'Aurora-1' nos Clones 05; 10 e 15 de umezeiro e no 'Okinawa', tanto em março quanto em julho, com as metodologias utilizadas. O 'Okinawa' induz crescimento mais rápido ao enxerto, de forma que o ponto máximo do comprimento é atingido em tempo menor.This study aimed to evaluate the tissue union and initial growth of 'Aurora-1' peach buds on mume clones (Prunus mume Sieb. et Zucc. and 'Okinawa' [Prunus persica (L. Batsch] propagated by herbaceous cuttings. Two experiments were carried out, being adopted the chip budding (March and chip budding modified (July. The results showed that accomplishment of 'Aurora-1' peach bud on mume Clones 05, 10 and 15 and 'Okinawa' is viable, in both periods, with the methodologies used. The 'Okinawa' induces faster growth to the bud and the maximum length point is reached in a short time.

  14. Host Plants of Xylosandrus mutilatus in Mississippi

    International Nuclear Information System (INIS)

    Stone, W.D.; Nebeker, T.E.; Gerard, P.D.

    2007-01-01

    Host range of Xylosandrus mutilatus (Blandford) in North America is reported here for the first time. Descriptive data such as number of attacks per host, size of stems at point of attacks, and height of attacks above ground are presented. Hosts observed in Mississippi were Acer rubrum L., Acer saccharum Marsh., Acer palmatum Thunb., Ostrya virginiana (Mill.) K. Koch., Cornus florida L., Fagus grandifolia Ehrh., Liquidamber styraciflua L., Carya spp., Liriodendron tulipifera L., Melia azedarach L., Pinus taeda L., Prunus serotina Ehrh., Prunus americana Marsh., Ulmus alata Michaux, and Vitus rotundifolia Michaux. Liquidamber styraciflua had significantly more successful attacks, significantly higher probability of attacks, and significantly higher number of adult beetles per host tree than did Carya spp., A. rubrum, and L. tulipifera. This information is relevant in determining the impact this exotic beetle may have in nurseries, urban areas, and other forestry systems where this beetle becomes established. (author) [es

  15. Self-compatible peach (Prunus persica) has mutant versions of the S haplotypes found in self-incompatible Prunus species.

    Science.gov (United States)

    Tao, Ryutaro; Watari, Akiko; Hanada, Toshio; Habu, Tsuyoshi; Yaegaki, Hideaki; Yamaguchi, Masami; Yamane, Hisayo

    2007-01-01

    This study demonstrates that self-compatible (SC) peach has mutant versions of S haplotypes that are present in self-incompatible (SI) Prunus species. All three peach S haplotypes, S (1), S (2), and S (2m), found in this study encode mutated pollen determinants, SFB, while only S (2m) has a mutation that affects the function of the pistil determinant S-RNase. A cysteine residue in the C5 domain of the S (2m)-RNase is substituted by a tyrosine residue, thereby reducing RNase stability. The peach SFB mutations are similar to the SFB mutations found in SC haplotypes of sweet cherry (P. avium) and Japanese apricot (P. mume). SFB (1) of the S (1) haplotype, a mutant version of almond (P. dulcis) S (k) haplotype, encodes truncated SFB due to a 155 bp insertion. SFB (2) of the S (2) and S (2m) haplotypes, both of which are mutant versions of the S (a) haplotype in Japanese plum (P. salicina), encodes a truncated SFB due to a 5 bp insertion. Thus, regardless of the functionality of the pistil determinant, all three peach S haplotypes are SC haplotypes. Our finding that peach has mutant versions of S haplotypes that function in almond and Japanese plum, which are phylogenetically close and remote species, respectively, to peach in the subfamily Prunoideae of the Roasaceae, provides insight into the SC/SI evolution in Prunus. We discuss the significance of SC pollen part mutation in peach with special reference to possible differences in the SI mechanisms between Prunus and Solanaceae.

  16. Breeding rootstocks for Prunus species: Advances in genetic and genomics of peach and cherry as a model

    Directory of Open Access Journals (Sweden)

    Verónica Guajardo

    2015-08-01

    Full Text Available Prunus rootstock is an important choice in optimizing productivity of grafted cultivars. Nevertheless, many Prunus rootstocks are notoriously intolerant to hypoxia which is caused by waterlogging and/or heavy soils. There is no available information to help select Prunus rootstocks that are tolerant to stress conditions such as root hypoxia caused by excess moisture. Information from genetic maps has demonstrated a high level of synteny among Prunus species, and this suggests that they all share a similar genomic structure. It should be possible to identify the genetic determinants involved in tolerance to hypoxia and other traits in Prunus rootstocks by applying methods to identify regions of the genome involved in the expression of important traits; these have been developed mainly in peach which is the model species for the genus. Molecular markers that are tightly linked to major genes would be useful in marker-assisted selection (MAS to optimize new rootstock selection. This article provides insight on the advances in the development of molecular markers, genetic maps, and gene identification in Prunus, mainly in peach; the aim is to provide a general approach for identifying the genetic determinants of hypoxia stress in rootstocks.

  17. Molecular Variability Among Isolates of Prunus Necrotic Ringspot Virus from Different Prunus spp.

    Science.gov (United States)

    Aparicio, F; Myrta, A; Di Terlizzi, B; Pallás, V

    1999-11-01

    ABSTRACT Viral sequences amplified by polymerase chain reaction from 25 isolates of Prunus necrotic ringspot virus (PNRSV), varying in the symptomatology they cause in six different Prunus spp., were analyzed for restriction fragment polymorphisms. Most of the isolates could be discriminated by using a combination of three different restriction enzymes. The nucleotide sequences of the RNA 4 of 15 of these isolates were determined. Sequence comparisons and phylogenetic analyses of the RNA 4 and coat proteins (CPs) revealed that all of the isolates clustered into three different groups, represented by three previously sequenced PNRSV isolates: PV32, PE5, and PV96. The PE5-type group was characterized by a 5' untranslated region that was clearly different from that of the other two groups. The PV32-type group was characterized by an extra hexanucleotide consisting of a duplication of the six immediately preceding nucleotides. Although most of the variability was observed in the first third of the CP, the amino acid residues in this region, which were previously thought to be functionally important in the replication cycle of the virus, were strictly conserved. No clear correlation with the type of symptom or host specificity could be observed. The validity of this grouping was confirmed when other isolates recently characterized by other authors were included in these analyses.

  18. Growth response of four species of Eastern hardwood tree seedlings exposed to ozone, acidic precipitation, and sulfur dioxide

    International Nuclear Information System (INIS)

    Davis, D.D. Skelly, J.M.

    1992-01-01

    In 1987 a study was conducted in controlled environment chambers to determine the foliar sensitivity of tree seedlings of eight species to ozone and acidic precipitation, and to determine the influence of leaf position on symptom severity. Jensen and Dochinger conducted concurrent similar studies in Continuously Stirred Tank Reactor (CSTR) chambers with ten species of forest trees. Based on the results of these initial studies, four species representing a range in foliar sensitivity to ozone were chosen: black cherry (Prunus serotina Ehrh.), red maple (Acer rubrum L.), northern red oak (Quercus rubra L.) and yellow-poplar (Liriodendron tulipifera L.). These species were also chosen because of their ecological and/or commercial importance in Pennsylvania. Seedlings were exposed in growth chambers simulated acid rain. In addition acute exposures to sulfur dioxide were conducted in a regime based on unpublished monitoring data collected near coal-fired power plants. The objective of this study was to determine if the pollutant treatments influenced the growth and productivity of seedlings of these four species. This information will help researchers and foresters understand the role of air pollution in productivity of eastern forests

  19. Wild Prunus Fruit Species as a Rich Source of Bioactive Compounds.

    Science.gov (United States)

    Mikulic-Petkovsek, Maja; Stampar, Franci; Veberic, Robert; Sircelj, Helena

    2016-08-01

    Sugars, organic acids, carotenoids, tocopherols, chlorophylls, and phenolic compounds were quantified in fruit of 4 wild growing Prunus species (wild cherry, bird cherry, blackthorn, and mahaleb cherry) using HPLC-DAD-MSn. In wild Prunus, the major sugars were glucose and fructose, whereas malic and citric acids dominated among organic acids. The most abundant classes of phenolic compounds in the analyzed fruit species were anthocyanins, flavonols, derivatives of cinnamic acids, and flavanols. Two major groups of anthocyanins measured in Prunus fruits were cyanidin-3-rutinoside and cyanidin-3-glucoside. Flavonols were represented by 19 derivatives of quercetin, 10 derivatives of kaempferol, and 2 derivatives of isorhamnetin. The highest total flavonol content was measured in mahaleb cherry and bird cherry, followed by blackthorn and wild cherry fruit. Total phenolic content varied from 2373 (wild cherry) to 11053 mg GAE per kg (bird cherry) and ferric reducing antioxidant power antioxidant activity from 7.26 to 31.54 mM trolox equivalents per kg fruits. © 2016 Institute of Food Technologists®

  20. Prunus hybrids rootstocks for flat peach

    Directory of Open Access Journals (Sweden)

    Pilar Legua

    2012-02-01

    Full Text Available Peach (Prunus persica L. is the most important stone fruit tree grown in Spain and is the second most important fruit crop in Europe. The influence of eight Prunus rootstocks (GF-677, Krymsk® 86, PADAC 97-36, PADAC 99-05, PADAC 9912-03, PADAC 0024-01, PAC 0021-01 and PAC 0022-01 on vigor, yield and fruit quality traits of 'UFO 3' flat peach cultivar was studied. The highest trunk cross sectional area was exhibited by GF-677 and the lowest by PADAC 99-05, while intermediate values were found on the other rootstocks. The highest yield efficiency was found on PADAC 99-05, PAC 0021-01, PAC 0022-01 and PADAC 0024-01 and the lowest was shown on Krymsk® 86. The fruit quality parameters measured were color, fruit and stone weights, equatorial diameter, pulp thickness, pulp yield, firmness, pH, soluble solids content and titratable acidity. 'UFO 3' grafted on GF-677 resulted in the largest fruit weight, while the smallest was on PADAC 99-05. Fruits of 'UFO 3' showed a tendency to have higher firmness, higher red colored skin and RI when grafted on PADAC 99-05.

  1. Basic RNases of wild almond (Prunus webbii): cloning and characterization of six new S-RNase and one "non-S RNase" genes.

    Science.gov (United States)

    Banović, Bojana; Surbanovski, Nada; Konstantinović, Miroslav; Maksimović, Vesna

    2009-03-01

    In order to investigate the S-RNase allele structure of a Prunus webbii population from the Montenegrin region of the Balkans, we analyzed 10 Prunus webbii accessions. We detected 10 different S-RNase allelic variants and obtained the nucleotide sequences for six S-RNases. The BLAST analysis showed that these six sequences were new Prunus webbii S-RNase alleles. It also revealed that one of sequenced alleles, S(9)-RNase, coded for an amino acid sequence identical to that for Prunus dulcis S(14)-RNase, except for a single conservative amino acid replacement in the signal peptide region. Another, S(3)-RNase, was shown to differ by only three amino acid residues from Prunus salicina Se-RNase. The allele S(7)-RNase was found to be inactive by stylar protein isoelectric focusing followed by RNase-specific staining, but the reason for the inactivity was not at the coding sequence level. Further, in five of the 10 analyzed accessions, we detected the presence of one active basic RNase (marked PW(1)) that did not amplify with S-RNase-specific DNA primers. However, it was amplified with primers designed from the PA1 RNase nucleotide sequence (basic "non-S RNase" of Prunus avium) and the obtained sequence showed high homology (80%) with the PA1 allele. Although homologs of PA1 "non-S RNases" have been reported in four other Prunus species, this is the first recorded homolog in Prunus webbii. The evolutionary implications of the data are discussed.

  2. Accelerated solvent extraction of carotenoids from: Tunisian Kaki (Diospyros kaki L.), peach (Prunus persica L.) and apricot (Prunus armeniaca L.).

    Science.gov (United States)

    Zaghdoudi, Khalil; Pontvianne, Steve; Framboisier, Xavier; Achard, Mathilde; Kudaibergenova, Rabiga; Ayadi-Trabelsi, Malika; Kalthoum-Cherif, Jamila; Vanderesse, Régis; Frochot, Céline; Guiavarc'h, Yann

    2015-10-01

    Extraction of carotenoids from biological matrices and quantifications remains a difficult task. Accelerated solvent extraction was used as an efficient extraction process for carotenoids extraction from three fruits cultivated in Tunisia: kaki (Diospyros kaki L.), peach (Prunus persica L.) and apricot (Prunus armeniaca L.). Based on a design of experiment (DoE) approach, and using a binary solvent consisting of methanol and tetrahydrofuran, we could identify the best extraction conditions as being 40°C, 20:80 (v:v) methanol/tetrahydrofuran and 5 min of extraction time. Surprisingly and likely due to the high extraction pressure used (103 bars), these conditions appeared to be the best ones both for extracting xanthophylls such as lutein, zeaxanthin or β-cryptoxanthin and carotenes such as β-carotene, which present quite different polarities. Twelve surface responses were generated for lutein, zeaxanthin, β-cryptoxanthin and β-carotene in kaki, peach and apricot. Further LC-MS analysis allowed comparisons in carotenoids profiles between the fruits. Copyright © 2015 Elsevier Ltd. All rights reserved.

  3. Optimization of microwave roasting of almond (Prunus dulcis)

    Science.gov (United States)

    Microwave (MW) almond roasting was investigated as an alternative to hot air (HA) roasting. Nonpareil almonds (Prunus dulcis) were roasted at 140°C in a convection oven for different times to achieve light, medium, and dark roasting levels. Several instrumental measurements were taken, establishin...

  4. Mapping X-Disease Phytoplasma Resistance in Prunus virginiana.

    Science.gov (United States)

    Lenz, Ryan R; Dai, Wenhao

    2017-01-01

    Phytoplasmas such as " Candidatus Phytoplasma pruni," the causal agent of X-disease of stone fruits, lack detailed biological analysis. This has limited the understanding of plant resistance mechanisms. Chokecherry ( Prunus virginiana L.) is a promising model to be used for the plant-phytoplasma interaction due to its documented ability to resist X-disease infection. A consensus chokecherry genetic map "Cho" was developed with JoinMap 4.0 by joining two parental maps. The new map contains a complete set of 16 linkage groups, spanning a genetic distance of 2,172 cM with an average marker density of 3.97 cM. Three significant quantitative trait loci (QTL) associated with X-disease resistance were identified contributing to a total of 45.9% of the phenotypic variation. This updated genetic linkage map and the identified QTL will provide the framework needed to facilitate molecular genetics, genomics, breeding, and biotechnology research concerning X-disease in chokecherry and other Prunus species.

  5. Mapping X-Disease Phytoplasma Resistance in Prunus virginiana

    Directory of Open Access Journals (Sweden)

    Ryan R. Lenz

    2017-11-01

    Full Text Available Phytoplasmas such as “Candidatus Phytoplasma pruni,” the causal agent of X-disease of stone fruits, lack detailed biological analysis. This has limited the understanding of plant resistance mechanisms. Chokecherry (Prunus virginiana L. is a promising model to be used for the plant-phytoplasma interaction due to its documented ability to resist X-disease infection. A consensus chokecherry genetic map “Cho” was developed with JoinMap 4.0 by joining two parental maps. The new map contains a complete set of 16 linkage groups, spanning a genetic distance of 2,172 cM with an average marker density of 3.97 cM. Three significant quantitative trait loci (QTL associated with X-disease resistance were identified contributing to a total of 45.9% of the phenotypic variation. This updated genetic linkage map and the identified QTL will provide the framework needed to facilitate molecular genetics, genomics, breeding, and biotechnology research concerning X-disease in chokecherry and other Prunus species.

  6. Enraizamento in vitro de porta-enxertos de Prunus In vitro rooting of Prunus rootstocks

    Directory of Open Access Journals (Sweden)

    Marcelo Rogalski

    2003-08-01

    Full Text Available Na micropropagação de Prunus sp., o enraizamento tem sido considerado uma fase crítica, pois determina a sobrevivência das plantas durante a aclimatização. Dentre os fatores importantes ao enraizamento in vitro, destacam-se o genótipo e as auxinas por serem determinantes na indução e na formação de raízes. O objetivo deste trabalho foi avaliar o efeito de diferentes concentrações de IBA no enraizamento in vitro dos porta-enxertos de espécies do gênero Prunus: cultivares Capdeboscq e GF677, e seleções VP411 e VP417. Para o enraizamento in vitro, brotos com 2-3cm de comprimento foram introduzidos em meio de Lepoivre suplementado com 0,1; 0,5; 1,0 e 2,0 mg.L-1 IBA. Observou-se que o porta-enxerto 'Capdeboscq' apresentou maior taxa de enraizamento e maior número de raízes in vitro, sendo superior aos demais genótipos quanto a estas características. O nível de 1,0 mg.L-1 de IBA esteve associado à maior taxa média de enraizamento (100%, 64% e 64,0%, respectivamente para os porta-enxertos 'Capdeboscq', 'GF677' e VP411. O nível de 2,0 mg.L-1 de IBA foi superior para a seleção VP417 com taxa de 64% de enraizamento. Para os porta-enxertos 'Capdeboscq' e 'GF677', o número máximo de raízes foi de 9,6 e 5,2 raízes por broto, respectivamente, em resposta ao nível de 2,0 mg.L-1 de IBA, enquanto as seleções VP411 e VP417 apresentaram o maior número de raízes (3,6 e 3,9, respectivamente em resposta ao nível de 1,0 mg.L-1 de IBA.In Prunus sp. micropropagation of rooting is considered a critical stage, since it determines the plant survival during the acclimatization. Among important factors associated with rooting, the genotype and the auxins are considered important in the induction and formation of roots. The objective of the present work was to evaluate the effect of different IBA on the in vitro rooting of Prunus rootstocks Capdeboscq and GF677, and the selections VP411 and VP417. For the in vitro rooting stage, shoots of

  7. Synteny conservation between two distantly-related Rosaceae genomes: Prunus (the stone fruits and Fragaria (the strawberry

    Directory of Open Access Journals (Sweden)

    Sargent Daniel J

    2008-06-01

    Full Text Available Abstract Background The Rosaceae encompass a large number of economically-important diploid and polyploid fruit and ornamental species in many different genera. The basic chromosome numbers of these genera are x = 7, 8 and 9 and all have compact and relatively similar genome sizes. Comparative mapping between distantly-related genera has been performed to a limited extent in the Rosaceae including a comparison between Malus (subfamily Maloideae and Prunus (subfamily Prunoideae; however no data has been published to date comparing Malus or Prunus to a member of the subfamily Rosoideae. In this paper we compare the genome of Fragaria, a member of the Rosoideae, to Prunus, a member of the Prunoideae. Results The diploid genomes of Prunus (2n = 2x = 16 and Fragaria (2n = 2x = 14 were compared through the mapping of 71 anchor markers – 40 restriction fragment length polymorphisms (RFLPs, 29 indels or single nucleotide polymorphisms (SNPs derived from expressed sequence tags (ESTs and two simple-sequence repeats (SSRs – on the reference maps of both genera. These markers provided good coverage of the Prunus (78% and Fragaria (78% genomes, with maximum gaps and average densities of 22 cM and 7.3 cM/marker in Prunus and 32 cM and 8.0 cM/marker in Fragaria. Conclusion Our results indicate a clear pattern of synteny, with most markers of each chromosome of one of these species mapping to one or two chromosomes of the other. A large number of rearrangements (36, most of which produced by inversions (27 and the rest (9 by translocations or fission/fusion events could also be inferred. We have provided the first framework for the comparison of the position of genes or DNA sequences of these two economically valuable and yet distantly-related genera of the Rosaceae.

  8. Prunasin Hydrolases during Fruit Development in Sweet and Bitter Almonds1[C][W][OA

    Science.gov (United States)

    Sánchez-Pérez, Raquel; Belmonte, Fara Sáez; Borch, Jonas; Dicenta, Federico; Møller, Birger Lindberg; Jørgensen, Kirsten

    2012-01-01

    Amygdalin is a cyanogenic diglucoside and constitutes the bitter component in bitter almond (Prunus dulcis). Amygdalin concentration increases in the course of fruit formation. The monoglucoside prunasin is the precursor of amygdalin. Prunasin may be degraded to hydrogen cyanide, glucose, and benzaldehyde by the action of the β-glucosidase prunasin hydrolase (PH) and mandelonitirile lyase or be glucosylated to form amygdalin. The tissue and cellular localization of PHs was determined during fruit development in two sweet and two bitter almond cultivars using a specific antibody toward PHs. Confocal studies on sections of tegument, nucellus, endosperm, and embryo showed that the localization of the PH proteins is dependent on the stage of fruit development, shifting between apoplast and symplast in opposite patterns in sweet and bitter cultivars. Two different PH genes, Ph691 and Ph692, have been identified in a sweet and a bitter almond cultivar. Both cDNAs are 86% identical on the nucleotide level, and their encoded proteins are 79% identical to each other. In addition, Ph691 and Ph692 display 92% and 86% nucleotide identity to Ph1 from black cherry (Prunus serotina). Both proteins were predicted to contain an amino-terminal signal peptide, with the size of 26 amino acid residues for PH691 and 22 residues for PH692. The PH activity and the localization of the respective proteins in vivo differ between cultivars. This implies that there might be different concentrations of prunasin available in the seed for amygdalin synthesis and that these differences may determine whether the mature almond develops into bitter or sweet. PMID:22353576

  9. Molecular characterization of the plum collection [Prunus domestica ...

    African Journals Online (AJOL)

    Eight Random Amplified Microsatellite markers (RAMs) were used to characterize the genetic diversity found in 14 Prunus materials belonging to the deciduous collection of the Pedagogical and Technological University of Colombia. A total of 121 bands were generated: they range from nine for the GT primer to 26 for the ...

  10. Analysis of Agromorphological Descriptors to Differentiate between Duke Cherry (Prunus x gondouinii (Poit. & TurpinRehd. and Its Progenitors: Sweet Cherry (Prunus avium L. and Sour Cherry (Prunus cerasus L. Análisis de Descriptores Agromorfológicos para Diferenciar entre Cerezo Duke (Prunus x gondouinii (Poit. & Turpin Rehd. y sus Progenitores: Cerezo (Prunus avium L. y Guindo (Prunus cerasus L.

    Directory of Open Access Journals (Sweden)

    Rodrigo Pérez

    2010-03-01

    Full Text Available The rapid identification of the hybrids between sweet cherry (Prunus avium L. and sour cherry (Prunus cerasus L. is not easy. In order to resolve this problem, 18 Spanish sweet, sour and duke cherry cultivars were surveyed and characterized using 43 agromorphological descriptors evaluated in flowers, leaves, dormant 1-yr-old shoots, fruits, and trees during 2005 and 2006. Based on quantitative parameters, ANOVA and stepwise discriminant analysis (SDA were carried out. For qualitative descriptors, statistical comparisons were done by means of the chi-square (χ2 test. As result of the study, two quantitative (titratable acidity and number of lenticels and six qualitative descriptors (shape of the central and lateral lobes in the internal bracts of the flower fascicles, leaf shape and margin, pubescence in the veins of the lower side of the leaf, and type of sulci of the seed coat were identified as differential parameters in P. avium, P. cerasus and P. x gondouinii(Poit. & Turpin Rehd. Also, another four qualitative descriptors (petal coloration at the end of blooming, leaf stipule type, and seed shape and viability were found to be useful for easy differentiation between sour and duke cherry. None of these parameters has been employed previously to discriminate among sweet, sour and duke cherry.Los híbridos de cerezo (Prunus avium L. y guindo (Prunus cerasus L. no son fáciles de identificar. Para resolver este problema, 18 cultivares de cerezo, guindo y sus híbridos fueron prospectados y caracterizados agromorfológicamente mediante el estudio de 43 descriptores evaluados en flores, hojas, frutos, ramas de 1 año y árbol durante los años 2005 y 2006. En base a los resultados obtenidos del estudio de los diferentes parámetros cuantitativos se realizaron un ANDEVA y un análisis discriminante escalonado (SDA. Los descriptores cualitativos fueron analizados mediante el test de Chi-cuadrado (χ². Como resultado del estudio se identificaron

  11. Slaat Xanthomonas dit jaar weer toe in Prunus laurocerasus

    NARCIS (Netherlands)

    Doorn, van J.; Dalfsen, van P.; Pham, K.T.K.

    2012-01-01

    Een diagnostische test moet duidelijk maken of er sprake is van Xanthomonas in Prunus laurocerasus. De bacterieziekte is namelijk makkelijk te verwarren met andere ziekten. Onderzoek, gefinancierd door het Productschap Tuinbouw, richt zich op het toetsen van moerplanten voordat hier van gestekt gaat

  12. Characterization of polymorphic SSRs among Prunus chloroplast genomes

    Science.gov (United States)

    An in silico mining process yielded 80, 75, and 78 microsatellites in the chloroplast genome of Prunus persica, P. kansuensis, and P. mume. A and T repeats were predominant in the three genomes, accounting for 67.8% on average and most of them were successful in primer design. For the 80 P. persica ...

  13. Prunus serotina unleashed: invader dominance after 70 years of forest development

    NARCIS (Netherlands)

    Vanhellemont, M.; Wauters, L.; Baeten, L.; Bijlsma, R.J.; Frenne, De P.; Hermy, M.; Verheyen, K.

    2010-01-01

    Propagule pressure and disturbance have both been found to facilitate invasion. Therefore, knowledge on the history of introduction and disturbance is vital for understanding an invasion process, and research should focus on areas in which the invasive species has not been deliberately introduced or

  14. Anatomy and cell wall polysaccharides of almond (Prunus dulcis D. A. Webb) seeds.

    Science.gov (United States)

    Dourado, Fernando; Barros, António; Mota, Manuel; Coimbra, Manuel A; Gama, Francisco M

    2004-03-10

    The anatomy of Prunus dulcis was analyzed by applying several differential staining techniques and light microscopy. Prunus dulcis seed has a thin and structurally complex seed coat, with lignified cellulosic tissue. The embryo has two voluminous cotyledons. Cotyledon cells have a high number of protein and lipid bodies, some of which have phytin. The provascular tissue, located in the cotyledons, is oriented in small bundles perpendicular to the transverse embryonic axis. Prunus dulcis cell wall material is very rich in arabinose (45 mol %). Glucose (23%), uronic acids (12%), and xylose (12%) are also major sugar components. The polymers obtained from the imidazole and Na(2)CO(3) extracts contain mainly pectic substances rich in arabinose, but the sugar content of these extracts was very low. The majority of the pectic substances (also rich in arabinose) was recovered with the KOH extracts. These extracts, with high sugar content, yielded also xyloglucans and acidic xylans. The 4 M KOH + H(3)BO(3) extracts yielded polysaccharides rich in uronic acids and xylose and very rich in arabinose, accounting for 27% of the cell wall material.

  15. Micropropagation of Prunus species relevant to cherry fruit production.

    Science.gov (United States)

    Druart, Philippe

    2013-01-01

    Cherry tree micropropagation is limited to the production of healthy cultivars of Prunus avium and Prunus cerasus, and their rootstocks; mainly the dwarfing ones. By using meristem-tip (0.1 mm long) or healthy shoot tips/nodes, four successive steps are needed to obtain whole plants capable of growing in the nursery: multiplication by axillary branching, shoot elongation, rooting, and plantlet acclimation. Along this process, several parameters have to be adjusted for each phase of the culture, including media composition, environmental culture conditions and plant handling. These parameters vary depending on genotypic response and specific vulnerability to physiological disorders such as hyperhydricity, apex necrosis, unstable propagation, and rooting rates. Based on a 40 year-long experience of study and application of culture conditions to large-scale plant production, this document summarizes the main problems (variability of the propagation rate, hyperhydricity, apex necrosis, plant re-growth) and solutions encountered to solve them, with means validated on many mericlones.

  16. Allelic diversity of S-RNase at the self-incompatibility locus in natural flowering cherry populations (Prunus lannesiana var. speciosa).

    Science.gov (United States)

    Kato, S; Mukai, Y

    2004-03-01

    In the Rosaceae family, which includes Prunus, gametophytic self-incompatibility (GSI) is controlled by a single multiallelic locus (S-locus), and the S-locus product expressed in the pistils is a glycoprotein with ribonuclease activity (S-RNase). Two populations of flowering cherry (Prunus lannesiana var. speciosa), located on Hachijo Island in Japan's Izu Islands, were sampled, and S-allele diversity was surveyed based on the sequence polymorphism of S-RNase. A total of seven S-alleles were cloned and sequenced. The S-RNases of flowering cherry showed high homology to those of Prunus cultivars (P. avium and P. dulcis). In the phylogenetic tree, the S-RNases of flowering cherry and other Prunus cultivars formed a distinct group, but they did not form species-specific subgroups. The nucleotide substitution pattern in S-RNases of flowering cherry showed no excess of nonsynonymous substitutions relative to synonymous substitutions. However, the S-RNases of flowering cherry had a higher Ka/Ks ratio than those of other Prunus cultivars, and a subtle heterogeneity in the nucleotide substitution rates was observed among the Prunus species. The S-genotype of each individual was determined by Southern blotting of restriction enzyme-digested genomic DNA, using cDNA for S-RNase as a probe. A total of 22 S-alleles were identified. All individuals examined were heterozygous, as expected under GSI. The allele frequencies were, contrary to the expectation under GSI, significantly unequal. The two populations studied showed a high degree of overlap, with 18 shared alleles. However, the allele frequencies differed considerably between the two populations.

  17. Effects of fertilization and rootstock on nutrient status and fruit set in sour cherry Prunus cerasus 'Stevnsbaer'

    DEFF Research Database (Denmark)

    Jensen, N. L.; Toldam-Andersen, Torben; Dencker, Ivar Blücher

    2007-01-01

    The purpose of this experiment was to investigate the effects of two potassium fertilization treatments on fruit set and flower bud, flower and bract leaf nutrient concentrations in Prunus cerasus 'Stevnsbaer' on Prunus avium and 'Colt' rootstocks. Single applications of KNO3 or KCl were applied ...

  18. A molecular phylogeny of selected species of genus Prunus L ...

    African Journals Online (AJOL)

    USER

    2010-08-02

    Aug 2, 2010 ... 52 - 56°C with the primers ITS-9 and ITS-6 or Trn-L and Trn-F. Polymerase chain .... The sub-genus Prunus has also relatively good support (81%) including .... Stevens, Michael J, Donoghue (1999). Plant Systematics. A.

  19. [Analysis of total proteins in the seed of almond (Prunus dulcis) by two-dimensional electrophoresis].

    Science.gov (United States)

    Li, Dong-dong; He, Shao-heng

    2004-07-01

    To analyse the total proteins in the seeds of almond (Prunus dulcis), one of the popular ingestent allergens in China, by two-dimensional electrophoresis. The total proteins of the seeds were extracted by trichloracetic acid (TCA) method, and then separated by isoelectric focusing as first dimension and SDS-PAGE as the second dimension. The spots of proteins were visualized by staining with Coomassie Brilliant Blue R-250. After analysis with software (ImageMaster 2D), 188 different proteins were detected. The isoelectric points (pI) for approximately 28% of total proteins were between 4.5-5.5, and the relative molecular mass (M(r)) of approximately 62% total proteins were between (20-25)x10(3). This was the first high-resolution, two-dimensional protein map of the seed of almond (Prunus dulcis) in China. Our finding has laid a solid foundation for further identification, characterization, gene cloning and standardization of allergenic proteins in the seed of almond (Prunus dulcis).

  20. Cryopreservation of in vitro -grown shoot tips of apricot ( Prunus ...

    African Journals Online (AJOL)

    In vitro grown apricot (Prunus armeniaca L.) cv. El-Hamawey shoot tips were successfully cryopreserved using an encapsulation-dehydration procedure. Shoot tips were encapsulated in calcium-alginate beads before preculture on woody plant (WP) medium supplemented with different sucrose concentrations; 0.1, 0.3, 0.5, ...

  1. Novel Rosaceae plant elicitor peptides as sustainable tools to control Xanthomonas arboricola pv. pruni in Prunus spp.

    Science.gov (United States)

    Ruiz, Cristina; Nadal, Anna; Montesinos, Emilio; Pla, Maria

    2018-02-01

    Fruit crops are regarded as important health promoters and constitute a major part of global agricultural production, and Rosaceae species are of high economic impact. Their culture is threatened by bacterial diseases, whose control is based on preventative treatments using compounds of limited efficacy and negative environmental impact. One of the most economically relevant examples is the pathogen Xanthomonas arboricola pv. pruni (Xap) affecting Prunus spp. The plant immune response against pathogens can be triggered and amplified by plant elicitor peptides (Peps), perceived by specific receptors (PEPRs). Although they have been described in various angiosperms, scarce information is available on Rosaceae species. Here, we identified the Pep precursor (PROPEP), Pep and PEPR orthologues of 10 Rosaceae species and confirmed the presence of the Pep/PEPR system in this family. We showed the perception and elicitor activity of Rosaceae Peps using the Prunus-Xap pathosystem as proof-of-concept. Treatment with nanomolar doses of Peps induced the corresponding PROPEP and a set of defence-related genes in Prunus leaves, and enhanced resistance against Xap. Peps from the same species had the highest efficiencies. Rosaceae Peps could potentially be used to develop natural, targeted and environmentally friendly strategies to enhance the resistance of Prunus species against biotic attackers. © 2017 BSPP AND JOHN WILEY & SONS LTD.

  2. Growth and fruit bearing of the sweet cherry (Prunus avium L

    African Journals Online (AJOL)

    Radunic

    2011-06-06

    Jun 6, 2011 ... Modern intensive production of sweet cherry (Prunus avium L.) tends to planting of high ... the highest was recorded on "V", while the smallest was in Spanish bush. Training system and density did not affect the fruit weight.

  3. Rapid detection of Prunus necrotic ringspot virus using magnetic nanoparticle-assisted reverse transcription loop-mediated isothermal amplification.

    Science.gov (United States)

    Zong, Xiaojuan; Wang, Wenwen; Wei, Hairong; Wang, Jiawei; Chen, Xin; Xu, Li; Zhu, Dongzi; Tan, Yue; Liu, Qingzhong

    2014-11-01

    Prunus necrotic ringspot virus (PNRSV) has seriously reduced the yield of Prunus species worldwide. In this study, a highly efficient and specific two-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) was developed to detect PNRSV. Total RNA was extracted from sweet cherry leaf samples using a commercial kit based on a magnetic nanoparticle technique. Transcripts were used as the templates for the assay. The results of this assay can be detected using agarose gel electrophoresis or by assessing in-tube fluorescence after adding SYBR Green I. The assay is highly specific for PNRSV, and it is more sensitive than reverse-transcription polymerase chain reaction (RT-PCR). Restriction enzyme digestion verified further the reliability of this RT-LAMP assay. To our knowledge, this is the first report of the application of RT-LAMP to PNRSV detection in Prunus species. Copyright © 2014 Elsevier B.V. All rights reserved.

  4. Looking into flowering time in almond (Prunus dulcis (Mill) D. A. Webb): the candidate gene approach.

    Science.gov (United States)

    Silva, C; Garcia-Mas, J; Sánchez, A M; Arús, P; Oliveira, M M

    2005-03-01

    Blooming time is one of the most important agronomic traits in almond. Biochemical and molecular events underlying flowering regulation must be understood before methods to stimulate late flowering can be developed. Attempts to elucidate the genetic control of this process have led to the identification of a major gene (Lb) and quantitative trait loci (QTLs) linked to observed phenotypic differences, but although this gene and these QTLs have been placed on the Prunus reference genetic map, their sequences and specific functions remain unknown. The aim of our investigation was to associate these loci with known genes using a candidate gene approach. Two almond cDNAs and eight Prunus expressed sequence tags were selected as candidate genes (CGs) since their sequences were highly identical to those of flowering regulatory genes characterized in other species. The CGs were amplified from both parental lines of the mapping population using specific primers. Sequence comparison revealed DNA polymorphisms between the parental lines, mainly of the single nucleotide type. Polymorphisms were used to develop co-dominant cleaved amplified polymorphic sequence markers or length polymorphisms based on insertion/deletion events for mapping the candidate genes on the Prunus reference map. Ten candidate genes were assigned to six linkage groups in the Prunus genome. The positions of two of these were compatible with the regions where two QTLs for blooming time were detected. One additional candidate was localized close to the position of the Evergrowing gene, which determines a non-deciduous behaviour in peach.

  5. Insights into the Prunus-Specific S-RNase-Based Self-Incompatibility System from a Genome-Wide Analysis of the Evolutionary Radiation of S Locus-Related F-box Genes.

    Science.gov (United States)

    Akagi, Takashi; Henry, Isabelle M; Morimoto, Takuya; Tao, Ryutaro

    2016-06-01

    Self-incompatibility (SI) is an important plant reproduction mechanism that facilitates the maintenance of genetic diversity within species. Three plant families, the Solanaceae, Rosaceae and Plantaginaceae, share an S-RNase-based gametophytic SI (GSI) system that involves a single S-RNase as the pistil S determinant and several F-box genes as pollen S determinants that act via non-self-recognition. Previous evidence has suggested a specific self-recognition mechanism in Prunus (Rosaceae), raising questions about the generality of the S-RNase-based GSI system. We investigated the evolution of the pollen S determinant by comparing the sequences of the Prunus S haplotype-specific F-box gene (SFB) with those of its orthologs in other angiosperm genomes. Our results indicate that the Prunus SFB does not cluster with the pollen S of other plants and diverged early after the establishment of the Eudicots. Our results further indicate multiple F-box gene duplication events, specifically in the Rosaceae family, and suggest that the Prunus SFB gene originated in a recent Prunus-specific gene duplication event. Transcriptomic and evolutionary analyses of the Prunus S paralogs are consistent with the establishment of a Prunus-specific SI system, and the possibility of subfunctionalization differentiating the newly generated SFB from the original pollen S determinant. © The Author 2016. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  6. Alternaria cerasidanica sp nov., isolated in Denmark from drupes of Prunus avium

    DEFF Research Database (Denmark)

    Roberts, R. G.; Reymond, S. T.; Andersen, Birgitte

    2010-01-01

    The ex-type strain of Alternaria cerasidanica was isolated in 2001 from an immature, asymptomatic drupe of Prunus avium collected at a commercial cherry orchard near Skaelskor, Denmark. Cultural morphology, sporulation pattern and cluster analyses of combined RAPD, RAMS (microsatellite), and AFLP...

  7. Mapping X-Disease Phytoplasma Resistance in Prunus virginiana

    OpenAIRE

    Ryan R. Lenz; Wenhao Dai

    2017-01-01

    Phytoplasmas such as “Candidatus Phytoplasma pruni,” the causal agent of X-disease of stone fruits, lack detailed biological analysis. This has limited the understanding of plant resistance mechanisms. Chokecherry (Prunus virginiana L.) is a promising model to be used for the plant-phytoplasma interaction due to its documented ability to resist X-disease infection. A consensus chokecherry genetic map “Cho” was developed with JoinMap 4.0 by joining two parental maps. The new map contains a com...

  8. Multiple Events of Allopolyploidy in the Evolution of the Racemose Lineages in Prunus (Rosaceae Based on Integrated Evidence from Nuclear and Plastid Data.

    Directory of Open Access Journals (Sweden)

    Liang Zhao

    Full Text Available Prunus is an economically important genus well-known for cherries, plums, almonds, and peaches. The genus can be divided into three major groups based on inflorescence structure and ploidy levels: (1 the diploid solitary-flower group (subg. Prunus, Amygdalus and Emplectocladus; (2 the diploid corymbose group (subg. Cerasus; and (3 the polyploid racemose group (subg. Padus, subg. Laurocerasus, and the Maddenia group. The plastid phylogeny suggests three major clades within Prunus: Prunus-Amygdalus-Emplectocladus, Cerasus, and Laurocerasus-Padus-Maddenia, while nuclear ITS trees resolve Laurocerasus-Padus-Maddenia as a paraphyletic group. In this study, we employed sequences of the nuclear loci At103, ITS and s6pdh to explore the origins and evolution of the racemose group. Two copies of the At103 gene were identified in Prunus. One copy is found in Prunus species with solitary and corymbose inflorescences as well as those with racemose inflorescences, while the second copy (II is present only in taxa with racemose inflorescences. The copy I sequences suggest that all racemose species form a paraphyletic group composed of four clades, each of which is definable by morphology and geography. The tree from the combined At103 and ITS sequences and the tree based on the single gene s6pdh had similar general topologies to the tree based on the copy I sequences of At103, with the combined At103-ITS tree showing stronger support in most clades. The nuclear At103, ITS and s6pdh data in conjunction with the plastid data are consistent with the hypothesis that multiple independent allopolyploidy events contributed to the origins of the racemose group. A widespread species or lineage may have served as the maternal parent for multiple hybridizations involving several paternal lineages. This hypothesis of the complex evolutionary history of the racemose group in Prunus reflects a major step forward in our understanding of diversification of the genus and has

  9. Tractor-mounted, GPS-based spot fumigation system manages Prunus replant disease

    Science.gov (United States)

    Our research goal was to use recent advances in global positioning system (GPS) and computer technology to apply just the right amount of fumigant where it is most needed (i.e., in a small target treatment zone in and around each tree replanting site) to control Prunus replant disease (PRD). We deve...

  10. Transcriptomic analysis of Prunus domestica undergoing hypersensitive response to plum pox virus infection.

    Science.gov (United States)

    Rodamilans, Bernardo; San León, David; Mühlberger, Louisa; Candresse, Thierry; Neumüller, Michael; Oliveros, Juan Carlos; García, Juan Antonio

    2014-01-01

    Plum pox virus (PPV) infects Prunus trees around the globe, posing serious fruit production problems and causing severe economic losses. One variety of Prunus domestica, named 'Jojo', develops a hypersensitive response to viral infection. Here we compared infected and non-infected samples using next-generation RNA sequencing to characterize the genetic complexity of the viral population in infected samples and to identify genes involved in development of the resistance response. Analysis of viral reads from the infected samples allowed reconstruction of a PPV-D consensus sequence. De novo reconstruction showed a second viral isolate of the PPV-Rec strain. RNA-seq analysis of PPV-infected 'Jojo' trees identified 2,234 and 786 unigenes that were significantly up- or downregulated, respectively (false discovery rate; FDR≤0.01). Expression of genes associated with defense was generally enhanced, while expression of those related to photosynthesis was repressed. Of the total of 3,020 differentially expressed unigenes, 154 were characterized as potential resistance genes, 10 of which were included in the NBS-LRR type. Given their possible role in plant defense, we selected 75 additional unigenes as candidates for further study. The combination of next-generation sequencing and a Prunus variety that develops a hypersensitive response to PPV infection provided an opportunity to study the factors involved in this plant defense mechanism. Transcriptomic analysis presented an overview of the changes that occur during PPV infection as a whole, and identified candidates suitable for further functional characterization.

  11. Transcriptomic analysis of Prunus domestica undergoing hypersensitive response to plum pox virus infection.

    Directory of Open Access Journals (Sweden)

    Bernardo Rodamilans

    Full Text Available Plum pox virus (PPV infects Prunus trees around the globe, posing serious fruit production problems and causing severe economic losses. One variety of Prunus domestica, named 'Jojo', develops a hypersensitive response to viral infection. Here we compared infected and non-infected samples using next-generation RNA sequencing to characterize the genetic complexity of the viral population in infected samples and to identify genes involved in development of the resistance response. Analysis of viral reads from the infected samples allowed reconstruction of a PPV-D consensus sequence. De novo reconstruction showed a second viral isolate of the PPV-Rec strain. RNA-seq analysis of PPV-infected 'Jojo' trees identified 2,234 and 786 unigenes that were significantly up- or downregulated, respectively (false discovery rate; FDR≤0.01. Expression of genes associated with defense was generally enhanced, while expression of those related to photosynthesis was repressed. Of the total of 3,020 differentially expressed unigenes, 154 were characterized as potential resistance genes, 10 of which were included in the NBS-LRR type. Given their possible role in plant defense, we selected 75 additional unigenes as candidates for further study. The combination of next-generation sequencing and a Prunus variety that develops a hypersensitive response to PPV infection provided an opportunity to study the factors involved in this plant defense mechanism. Transcriptomic analysis presented an overview of the changes that occur during PPV infection as a whole, and identified candidates suitable for further functional characterization.

  12. Immunochemical and biological properties of a mouse monoclonal antibody reactive to prunus necrotic ringspot ilarvirus.

    Science.gov (United States)

    Aebig, J A; Jordan, R L; Lawson, R H; Hsu, H T

    1987-01-01

    A monoclonal antibody reacting with prunus necrotic ringspot ilarvirus was tested in immunochemical studies, neutralization of infectivity assays, and by immuno-electron microscopy. The antibody was able to detect the 27,000 Mr coat protein of prunus necrotic ringspot ilarvirus in western blots and also detected all polypeptide fragments generated after incubation of whole virus with proteolytic enzymes. In neutralization of infectivity studies, the antibody blocked virus infectivity, although it did not precipitate the antigen in agar gel Ouchterlony double diffusion tests. Immuno-electron microscopy confirmed that the antibody coats virions but does not cause clumping. The antibody may be a useful tool for investigating coat protein-dependent initiation of ilarvirus infection.

  13. Multiyear evaluation of the durability of the resistance conferred by Ma and RMia genes to Meloidogyne incognita in Prunus under controlled conditions.

    Science.gov (United States)

    Khallouk, Samira; Voisin, Roger; Portier, Ulysse; Polidori, Joël; Van Ghelder, Cyril; Esmenjaud, Daniel

    2013-08-01

    Root-knot nematodes (RKNs) (Meloidogyne spp.) are highly polyphagous pests that parasitize Prunus crops in Mediterranean climates. Breeding for RKN-resistant Prunus cultivars, as an alternative to the now-banned use of nematicides, is a real challenge, because the perennial nature of these trees increases the risk of resistance breakdown. The Ma plum resistance (R) gene, with a complete spectrum, and the RMia peach R gene, with a more restricted spectrum, both provide total control of Meloidogyne incognita, the model parthenogenetic species of the genus and the most important RKN in terms of economic losses. We investigated the durability of the resistance to this nematode conferred by these genes, comparing the results obtained with those for the tomato Mi-1 reference gene. In multiyear experiments, we applied a high and continuous nematode inoculum pressure by cultivating nematode-infested susceptible tomato plants with either Prunus accessions carrying Ma or RMia R genes, or with resistant tomato plants carrying the Mi-1 gene. Suitable conditions for Prunus development were achieved by carrying out the studies in a glasshouse, in controlled conditions allowing a short winter leaf fall and dormancy. We first assessed the plum accession 'P.2175', which is heterozygous for the Ma gene, in two successive 2-year evaluations, for resistance to two M. incognita isolates. Whatever the isolate used, no nematodes reproducing on P.2175 were detected, whereas galls and nematodes reproducing on tomato plants carrying Mi-1 were observed. In a second experiment with the most aggressive isolate, interspecific full-sib material (P.2175 × ['Garfi' almond × 'Nemared' peach]), carrying either Ma or RMia (from Nemared) or both (in the heterozygous state) or neither of these genes, was evaluated for 4 years. No virulent nematodes developed on Prunus spp. carrying R genes, whereas galling and virulent individuals were observed on Mi-1-resistant tomato plants. Thus, the resistance to

  14. Rapid Detection of Prunus Necrotic Ringspot Virus by Reverse Transcription-cross-priming Amplification Coupled with Nucleic Acid Test Strip Cassette.

    Science.gov (United States)

    Huo, Ya-Yun; Li, Gui-Fen; Qiu, Yan-Hong; Li, Wei-Min; Zhang, Yong-Jiang

    2017-11-23

    Prunus necrotic ringspot virus (PNRSV) is one of the most devastating viruses to Prunus spp. In this study, we developed a diagnostic system RT-CPA-NATSC, wherein reverse transcription-cross-priming amplification (RT-CPA) is coupled with nucleic acid test strip cassette (NATSC), a vertical flow (VF) visualization, for PNRSV detection. The RT-CPA-NATSC assay targets the encoding gene of the PNRSV coat protein with a limit of detection of 72 copies per reaction and no cross-reaction with the known Prunus pathogenic viruses and viroids, demonstrating high sensitivity and specificity. The reaction is performed on 60 °C and can be completed less than 90 min with the prepared template RNA. Field sample test confirmed the reliability of RT-CPA-NATSC, indicating the potential application of this simple and rapid detection method in routine test of PNRSV.

  15. A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica for Functional Genomics Analyses

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    LEE MEISEL

    2005-01-01

    Full Text Available Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica. Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the fruits of Prunus persica. This RNA may be used for many functional genomic based experiments such as RT-PCR and the construction of large-insert cDNA libraries.

  16. Extracts of Canadian first nations medicinal plants, used as natural products, inhibit neisseria gonorrhoeae isolates with different antibiotic resistance profiles.

    Science.gov (United States)

    Cybulska, Paulina; Thakur, Sidharath D; Foster, Brian C; Scott, Ian M; Leduc, Renée I; Arnason, John T; Dillon, Jo-Anne R

    2011-07-01

    Neisseria gonorrhoeae (Ng) has developed resistance to most antimicrobial agents and the antibiotics recommended for therapy are restricted, for the most part, to third generation cephalosporins. In order to investigate new potential sources of antimicrobial agents, the antibacterial properties of 14 Canadian plants used in traditional First Nations' medicine were tested against Ng isolates having differing antimicrobial susceptibility profiles. Ethanolic extracts of 14 Canadian botanicals, analyzed by high-performance liquid chromatography, were tested for their antimicrobial activity (disc diffusion and/or agar dilution assays) against susceptible Ng reference strains and a panel of 28 Ng isolates with various antimicrobial resistance profiles. Extracts of Arctostaphylos uva ursi (kinnikinnick or bearberry), Hydrastis canadensis (goldenseal), Prunus serotina (black cherry), and Rhodiola rosea (roseroot) inhibited the growth of all Ng isolates with minimum inhibitory concentrations of 32 μg/mL, 4 to 32 μg/mL, 16 to >32 μg/mL, and 32 to 64 μg/mL, respectively. Extracts of Acorus americanus (sweet flag), Berberis vulgaris (barberry), Cimicifuga racemosa (black cohosh), Equisetum arvense (field horsetail), Gaultheria procumbens (wintergreen), Ledum groenlandicum (Labrador tea), Ledum palustre (marsh Labrador tea), Oenothera biennis (common evening primrose), Sambucus nigra (elderberry), and Zanthoxylum americanum (prickly ash) had weak or no antimicrobial activity against the Ng isolates with minimum inhibitory concentrations ≥256 μg/mL. The phytochemical berberine from H. canadensis inhibited the growth of all Ng isolates. The phytochemicals, salidroside and rosavin, present in R. rosea, also showed inhibitory activity against Ng strains. Canadian botanicals represent a potential source of novel compounds which inhibit Ng, including isolates resistant to antibiotics.

  17. Mining microsatellites in the peach genome: development of new long-core SSR markers for genetic analyses in five Prunus species.

    Science.gov (United States)

    Dettori, Maria Teresa; Micali, Sabrina; Giovinazzi, Jessica; Scalabrin, Simone; Verde, Ignazio; Cipriani, Guido

    2015-01-01

    A wide inventory of molecular markers is nowadays available for individual fingerprinting. Microsatellites, or simple sequence repeats (SSRs), play a relevant role due to their relatively ease of use, their abundance in the plant genomes, and their co-dominant nature, together with the availability of primer sequences in many important agricultural crops. Microsatellites with long-core motifs are more easily scored and were adopted long ago in human genetics but they were developed only in few crops, and Prunus species are not among them. In the present work the peach whole-genome sequence was used to select 216 SSRs containing long-core motifs with tri-, tetra- and penta-nucleotide repeats. Microsatellite primer pairs were designed and tested for polymorphism in the five diploid Prunus species of economic relevance (almond, apricot, Japanese plum, peach and sweet cherry). A set of 26 microsatellite markers covering all the eight chromosomes, was also selected and used in the molecular characterization, population genetics and structure analyses of a representative sample of the five diploid Prunus species, assessing their transportability and effectiveness. The combined probability of identity between two random individuals for the whole set of 26 SSRs was quite low, ranging from 2.30 × 10(-7) in peach to 9.48 × 10(-10) in almond, confirming the usefulness of the proposed set for fingerprinting analyses in Prunus species.

  18. Promotion of adventitious root formation of difficult-to-root hardwood tree species

    Science.gov (United States)

    Paula M. Pijut; Keith E. Woeste; Charles H. Michler

    2011-01-01

    North American hardwood tree species, such as alder (Alnus spp.), ash (Fraxinus spp.), basswood (Tilia spp.), beech (Fagus spp.), birch (Betula spp.), black cherry (Prunus seratina), black walnut (Juglans nigra), black willow (...

  19. Genome-wide association links candidate genes to resistance to Plum Pox Virus in apricot (Prunus armeniaca).

    Science.gov (United States)

    Mariette, Stéphanie; Wong Jun Tai, Fabienne; Roch, Guillaume; Barre, Aurélien; Chague, Aurélie; Decroocq, Stéphane; Groppi, Alexis; Laizet, Yec'han; Lambert, Patrick; Tricon, David; Nikolski, Macha; Audergon, Jean-Marc; Abbott, Albert G; Decroocq, Véronique

    2016-01-01

    In fruit tree species, many important traits have been characterized genetically by using single-family descent mapping in progenies segregating for the traits. However, most mapped loci have not been sufficiently resolved to the individual genes due to insufficient progeny sizes for high resolution mapping and the previous lack of whole-genome sequence resources of the study species. To address this problem for Plum Pox Virus (PPV) candidate resistance gene identification in Prunus species, we implemented a genome-wide association (GWA) approach in apricot. This study exploited the broad genetic diversity of the apricot (Prunus armeniaca) germplasm containing resistance to PPV, next-generation sequence-based genotyping, and the high-quality peach (Prunus persica) genome reference sequence for single nucleotide polymorphism (SNP) identification. The results of this GWA study validated previously reported PPV resistance quantitative trait loci (QTL) intervals, highlighted other potential resistance loci, and resolved each to a limited set of candidate genes for further study. This work substantiates the association genetics approach for resolution of QTL to candidate genes in apricot and suggests that this approach could simplify identification of other candidate genes for other marked trait intervals in this germplasm. © 2015 INRA, UMR 1332 BFP New Phytologist © 2015 New Phytologist Trust.

  20. Evaluation of the hydroxynitrile lyase activity in cell cultures of capulin (Prunus serotina).

    Science.gov (United States)

    Hernández, Liliana; Luna, Héctor; Navarro-Ocaña, Arturo; Olivera-Flores, Ma Teresa de Jesús; Ayala, Ivon

    2008-07-01

    Enzymatic preparations obtained from young plants and cell cultures of capulin were screened for hydroxynitrile lyase activity. The three week old plants, grown under sterile conditions, were used to establish a solid cell culture. Crude preparations obtained from this plant material were evaluated for the transformation of benzaldehyde to the corresponding cyanohydrin (mandelonitrile). The results show that the crude material from roots, stalks, and leaves of young plants and calli of roots, stalks, internodes and petioles biocatalyzed the addition of hydrogen cyanide (HCN) to benzaldehyde with a modest to excellent enantioselectivity.

  1. Kirsipuu (Prunus avium) : [luuletused] / R. W. Stedingh ; tlk. ja saatesõna: Jüri Talvet

    Index Scriptorium Estoniae

    Stedingh, R. W.

    2003-01-01

    Sisu: Kirsipuu (Prunus avium) ; Rubus spectabilis ; Rododendron (Rhododendron macrophyllum) ; Lysuchitum americanum ; Tulp (Tulipa gesneriana) ; Kanada hani (Branta canadensis) ; Metsorava pärastlõuna (Sciurus carolinensis) ; Ohakalind (Spinus tristis) ; Shakespeare'i mälestusmärk (kogust "Stanley pargi süit")

  2. Brown Rot Strikes Prunus Fruit: An Ancient Fight Almost Always Lost.

    Science.gov (United States)

    Oliveira Lino, Leandro; Pacheco, Igor; Mercier, Vincent; Faoro, Franco; Bassi, Daniele; Bornard, Isabelle; Quilot-Turion, Bénédicte

    2016-05-25

    Brown rot (BR) caused by Monilinia spp., has been an economic problem for the stone fruit market due to dramatic losses, mainly during the postharvest period. There is much literature about basic aspects of Monilinia spp. infection, which indicates that environment significantly influences its occurrence in the orchard. However, progress is needed to sustainably limit this disease: the pathogen is able to develop resistance to pesticides, and most of BR resistance research programs in plant models perish. Solving this problem becomes important due to the need to decrease chemical treatments and reduce residues on fruit. Thus, research has recently increased, exploring a wide range of disease control strategies (e.g., genetic, chemical, physical). Summarizing this information is difficult, as studies evaluate different Monilinia and Prunus model species, with diverse strategies and protocols. Thus, the purpose of this review is to present the diversity and distribution of agents causing BR, focusing on the biochemical mechanisms of Monilinia spp. infection both of the fungi and of the fruit, and report on the resistance sources in Prunus germplasm. This review comprehensively compiles the information currently available to better understand mechanisms related to BR resistance.

  3. Preparation of recombinant coat protein of Prunus necrotic ringspot virus.

    Science.gov (United States)

    Petrzik, K; Mráz, I; Kubelková, D

    2001-02-01

    The coat protein (CP) gene of Prunus necrotic ringspot virus (PNRSV) was cloned into pET 16b vector and expressed in Escherichia coli. CP-enriched fractions were prepared from whole cell lysate by differential centrifugation. The fraction sedimenting at 20,000 x g for 30 mins was used for preparation of a rabbit antiserum to CP. This antiserum had a titer of 1:2048 and reacted in a double-antibody sandwich ELISA (DAS-ELISA).

  4. Formulation and quality control of Prunus domestica syrup, prepared according to Iranian Traditional Medicine

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    M. Hamzeloo-Moghadam

    2015-05-01

    Full Text Available Background and objectives: Prunus domestica (plum has been considered as a useful remedy for several disorders in Iranian Traditional Medicine (ITM. It has cold and wet temperament and is used as syrup for hot temperament diseases such as hot headache and stomach disorders. In the present study, plum syrup has been formulated according to ITM manuscripts and quality control evaluations have been accomplished to present a suitable formulation. Methods: The fruits of Prunus domestica L. were macerated in water, then decocted. The mixture was filtered. The filtrate was concentrated to have a suitable viscosity. The extract was sweetened by adding sugar (1:2 and heated till sugar was completely dissolved. The final product was evaluated physicochemically and microbiologically according to standard protocols and total phenolics content of the syrup stability was determined. The syrup was assessed in accelerated condition (40 ºC during 6 months. Results: The prepared formulation was a viscose and brown syrup with plum flavor and fragrance. No precipitation and cap locking were observed in the syrup. Dry residue, pH, density, viscosity and total phenolics of the syrup were found 43.1%, 3.49, 1.27 g/ml, 6.5 cP and 152.3 mg/100ml, respectively. No microbial growth was observed in the formulation. In the accelerated stability tests, no remarkable changes were seen in the product. Total phenolics content was decreased 2.2% during 6 months in 40 ºC. Conclusion: The formulated Prunus domestica syrup could be introduced for further mass production after completing the final required evaluations.

  5. Industrial processing effects on phenolic compounds in sour cherry (Prunus cerasus L.) fruit

    NARCIS (Netherlands)

    Toydemir, G.; Capanoglu, E.; Gomez-Roldan, M.V.; Vos, de R.C.H.; Boyacioglu, D.; Hall, R.D.; Beekwilder, M.J.

    2013-01-01

    The processed juice (or nectar) of the sour cherry, Prunus cerasus L., is widely consumed in the Balkan region and Turkey. Sour cherry is known to be rich in polyphenolic compounds, such as anthocyanins and procyanidins. In this work, the effects of processing of sour cherry fruit to nectar on

  6. Preliminary Guide to the Onsite Identification and Delineation of the Wetlands of the South Atlantic United States.

    Science.gov (United States)

    1982-05-01

    shield) Ceratophyilum demersum (Coontail) Myriophyllum spp. (Watermilfoil) Nelumbo lutea ( American lotus) Nuphar luteum (Spatterdock) Nymphaea odorata...Overcup oak) Quercus palustris (Pin oak) Quercus phelios (Willow oak) Ulmus americana ( American elm) UlmuS rubra (Slippery elm) c. Atlantic White...var. biflora (Swamp black gum) Persea borbonia (Red bay) Pinus serotina (Pond pine) Smilax laurifolia (Bamboo vine) Sphagnum spp. (Sphagnum moss

  7. Analysis of expressed sequence tags from Prunus mume flower and fruit and development of simple sequence repeat markers

    Directory of Open Access Journals (Sweden)

    Gao Zhihong

    2010-07-01

    Full Text Available Abstract Background Expressed Sequence Tag (EST has been a cost-effective tool in molecular biology and represents an abundant valuable resource for genome annotation, gene expression, and comparative genomics in plants. Results In this study, we constructed a cDNA library of Prunus mume flower and fruit, sequenced 10,123 clones of the library, and obtained 8,656 expressed sequence tag (EST sequences with high quality. The ESTs were assembled into 4,473 unigenes composed of 1,492 contigs and 2,981 singletons and that have been deposited in NCBI (accession IDs: GW868575 - GW873047, among which 1,294 unique ESTs were with known or putative functions. Furthermore, we found 1,233 putative simple sequence repeats (SSRs in the P. mume unigene dataset. We randomly tested 42 pairs of PCR primers flanking potential SSRs, and 14 pairs were identified as true-to-type SSR loci and could amplify polymorphic bands from 20 individual plants of P. mume. We further used the 14 EST-SSR primer pairs to test the transferability on peach and plum. The result showed that nearly 89% of the primer pairs produced target PCR bands in the two species. A high level of marker polymorphism was observed in the plum species (65% and low in the peach (46%, and the clustering analysis of the three species indicated that these SSR markers were useful in the evaluation of genetic relationships and diversity between and within the Prunus species. Conclusions We have constructed the first cDNA library of P. mume flower and fruit, and our data provide sets of molecular biology resources for P. mume and other Prunus species. These resources will be useful for further study such as genome annotation, new gene discovery, gene functional analysis, molecular breeding, evolution and comparative genomics between Prunus species.

  8. Genome-Wide Analysis Suggests the Relaxed Purifying Selection Affect the Evolution of WOX Genes in Pyrus bretschneideri, Prunus persica, Prunus mume, and Fragaria vesca

    Directory of Open Access Journals (Sweden)

    Yunpeng Cao

    2017-06-01

    Full Text Available WUSCHEL-related homeobox (WOX family is one of the largest group of transcription factors (TFs specifically found in plant kingdom. WOX TFs play an important role in plant development processes and evolutionary novelties. Although the roles of WOXs in Arabidopsis and rice have been well-studied, however, little are known about the relationships among the main clades in the molecular evolution of these genes in Rosaceae. Here, we carried out a genome-wide analysis and identified 14, 10, 10, and 9 of WOX genes from four Rosaceae species (Fragaria vesca, Prunus persica, Prunus mume, and Pyrus bretschneideri, respectively. According to evolutionary analysis, as well as amino acid sequences of their homodomains, these genes were divided into three clades with nine subgroups. Furthermore, due to the conserved structural patterns among these WOX genes, it was proposed that there should exist some highly conserved regions of microsynteny in the four Rosaceae species. Moreover, most of WOX gene pairs were presented with the conserved orientation among syntenic genome regions. In addition, according to substitution models analysis using PMAL software, no significant positive selection was detected, but type I functional divergence was identified among certain amino acids in WOX protein. These results revealed that the relaxed purifying selection might be the main driving force during the evolution of WOX genes in the tested Rosaceae species. Our result will be useful for further precise research on evolution of the WOX genes in family Rosaceae.

  9. Coniochaeta (Lecythophora), Collophora gen. nov. And Phaeomoniella species associated with wood necroses of Prunus trees

    NARCIS (Netherlands)

    Damm, U.; Fourie, P.H.; Crous, P.W.

    2010-01-01

    Species of the genus Coniochaeta (anamorph: Lecythophora) are known as pathogens of woody hosts, but can also cause opportunistic human infections. Several fungi with conidial stages resembling Lecythophora were isolated from necrotic wood samples of Prunus trees in South Africa. In order to reveal

  10. Caracterização de três genótipos de umezeiro (Prunus mume Sieb. et Zucc. por marcadores RAPD Characterization of three mume genotypes (Prunus mume Sieb. et Zucc. by RAPD markers

    Directory of Open Access Journals (Sweden)

    Newton Alex Mayer

    2008-12-01

    Full Text Available Um projeto de pesquisa visando à utilização de clones de umezeiro (Prunus mume Sieb. et Zucc. como porta-enxertos para pessegueiro [Prunus persica (L. Batsch] está sendo conduzido na FCAV/UNESP, Câmpus de Jaboticabal-SP, com promissoras perspectivas de sucesso. Três genótipos de umezeiro foram selecionados de acordo com características agronômicas desejáveis para esta finalidade. A distinção dos três genótipos entre si, baseada exclusivamente em características morfológicas, apresenta limitações. Dessa forma, o objetivo do presente trabalho foi identificar marcadores RAPD capazes de diferenciar e caracterizar os Clones 05, 15 e a cv. Rigitano (Clone 10 de umezeiro, utilizando-se das cultivares Aurora-1 e Okinawa de pessegueiro como outgroup. Dos 220 primers testados, foram selecionados 42, que amplificaram todos os cinco genótipos. Verificou-se que os marcadores RAPD permitiram a distinção entre o Clone 05, o Clone 15 e a cv. Rigitano de umezeiro, demonstrando a existência de variabilidade genética entre os mesmos. Dentre os três genótipos de umezeiro estudados, constatou-se que a similaridade genética é maior entre o Clone 05 e o Clone 15.A research project with the objective do develop mume clones (Prunus mume Sieb. et Zucc., to be used as rootstocks for peach tree [Prunus persica (L. Batsch] is been carried out at the Faculdade de Ciências Agrárias e Veterinárias (FCAV/UNESP, Jaboticabal Campus, São Paulo State, Brazil. These project showed promising perspectives of success, with three clones that were selected according to their characteristics for peach rootstock. But the distinction of the three clones among them, based only in morphologic characteristics, has presented limitations. The objective of the present research was to identify RAPD markers able to characterize and differentiate the 05 and 15 Clones and Rigitano mume cultivar, using Aurora-1 and Okinawa peach tree as outgroup. Among the 220 tested

  11. Whole-Genome Characterization of Prunus necrotic ringspot virus Infecting Sweet Cherry in China.

    Science.gov (United States)

    Wang, Jiawei; Zhai, Ying; Zhu, Dongzi; Liu, Weizhen; Pappu, Hanu R; Liu, Qingzhong

    2018-03-01

    Prunus necrotic ringspot virus (PNRSV) causes yield loss in most cultivated stone fruits, including sweet cherry. Using a small RNA deep-sequencing approach combined with end-genome sequence cloning, we identified the complete genomes of all three PNRSV strands from PNRSV-infected sweet cherry trees and compared them with those of two previously reported isolates. Copyright © 2018 Wang et al.

  12. Molecular characterization of peach [Prunus persica (L.) Batsch] germplasm in the United States using microsatellite markers

    Science.gov (United States)

    Peach [Prunus persica (L.) Batsch] is an important medicinal fruit with immense health benefits and antioxidant activity. In this study, microsatellite markers were used as DNA fingerprinting tools for the identification and characterization of peach germplasm in the United States. Eleven microsatel...

  13. In situ volatiles from a single cultivar of Prunus dulcis and their relationship to navel orangeworm

    Science.gov (United States)

    Nonpareil almonds, Prunus dulcis, account for the largest percentage of almond varieties grown in the Central and San Joaquin valleys of California. Several studies have investigated the various non-volatile and volatile components of various plant parts; however, the volatile organic compound (VOC)...

  14. Endogenous hormones response to cytokinins with regard to organogenesis in explants of peach (Prunus persica L. Batsch) cultivars and rootstocks (P. persica × Prunus dulcis).

    Science.gov (United States)

    Pérez-Jiménez, Margarita; Cantero-Navarro, Elena; Pérez-Alfocea, Francisco; Cos-Terrer, José

    2014-11-01

    Organogenesis in peach (Prunus persica L. Batsch) and peach rootstocks (P. persica × Prunus dulcis) has been achieved and the action of the regeneration medium on 7 phytohormones, zeatin (Z), zeatin riboside (ZR), indole-3-acetic acid (IAA), abscisic acid (ABA), ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), and jasmonic acid (JA), has been studied using High performance liquid chromatography - mass spectrometry (HPLC-MS/MS). Three scion peach cultivars, 'UFO-3', 'Flariba' and 'Alice Bigi', and the peach × almond rootstocks 'Garnem' and 'GF677' were cultured in two different media, Murashige and Skoog supplemented with plant growth regulators (PGRs) (regeneration medium) and without PGRs (control medium), in order to study the effects of the media and/or genotypes in the endogenous hormones content and their role in organogenesis. The highest regeneration rate was obtained with the peach × almond rootstocks and showed a lower content of Z, IAA, ABA, ACC and JA. Only Z, ZR and IAA were affected by the action of the culture media. This study shows which hormones are external PGRs-dependent and what is the weight of the genotype and hormones in peach organogenesis that provide an avenue to manipulate in vitro organogenesis in peach. Copyright © 2014 Elsevier Masson SAS. All rights reserved.

  15. Tests for Transmission of Prunus Necrotic Ringspot and Two Nepoviruses by Criconemella xenoplax.

    Science.gov (United States)

    Yuan, W Q; Barnett, O W; Westcott, S W; Scott, S W

    1990-10-01

    In two of three trials, detectable color reactions in ELISA for Prunus necrotic ringspot virus (PNRSV) were observed for Criconemella xenoplax handpicked from the root zone of infected peach trees. Criconemella xenoplax (500/pot) handpicked from root zones of peach trees infected with PNRSV failed to transmit the virus to cucumber or peach seedlings. The nematode also failed to transmit tomato ringspot (TomRSV) or tobacco ringspot viruses between cucumbers, although Xiphinema americanum transmitted TomRSV under the same conditions. Plants of peach, cucumber, Chenopodium quinoa, and Catharanthus roseus were not infected by PNRSV when grown in soil containing C. xenoplax collected from root zones of PNRSV-infected trees. Shirofugen cherry scions budded on Mazzard cherry seedling rootstocks remained symptomless when transplanted into root zones of PNRSV-infected trees. Virus transmission was not detected by ELISA when C. xenoplax individuals were observed to feed on cucumber root explants that were infected with PNRSV and subsequently fed on roots of Prunus besseyi in agar cultures. Even if virus transmission by C. xenoplax occurs via contamination rather than by a specific mechanism, it must be rare.

  16. Genetic and molecular characterization of three novel S-haplotypes in sour cherry (Prunus cerasus L.).

    Science.gov (United States)

    Tsukamoto, Tatsuya; Potter, Daniel; Tao, Ryutaro; Vieira, Cristina P; Vieira, Jorge; Iezzoni, Amy F

    2008-01-01

    Tetraploid sour cherry (Prunus cerasus L.) exhibits gametophytic self-incompatibility (GSI) whereby the specificity of self-pollen rejection is controlled by alleles of the stylar and pollen specificity genes, S-RNase and SFB (S haplotype-specific F-box protein gene), respectively. As sour cherry selections can be either self-compatible (SC) or self-incompatible (SI), polyploidy per se does not result in SC. Instead the genotype-dependent loss of SI in sour cherry is due to the accumulation of non-functional S-haplotypes. The presence of two or more non-functional S-haplotypes within sour cherry 2x pollen renders that pollen SC. Two new S-haplotypes from sour cherry, S(33) and S(34), that are presumed to be contributed by the P. fruticosa species parent, the complete S-RNase and SFB sequences of a third S-haplotype, S(35), plus the presence of two previously identified sweet cherry S-haplotypes, S(14) and S(16) are described here. Genetic segregation data demonstrated that the S(16)-, S(33)-, S(34)-, and S(35)-haplotypes present in sour cherry are fully functional. This result is consistent with our previous finding that 'hetero-allelic' pollen is incompatible in sour cherry. Phylogenetic analyses of the SFB and S-RNase sequences from available Prunus species reveal that the relationships among S-haplotypes show no correspondence to known organismal relationships at any taxonomic level within Prunus, indicating that polymorphisms at the S-locus have been maintained throughout the evolution of the genus. Furthermore, the phylogenetic relationships among SFB sequences are generally incongruent with those among S-RNase sequences for the same S-haplotypes. Hypotheses compatible with these results are discussed.

  17. Changes in sour cherry (Prunus cerasus L.) antioxidants during nectar processing and in vitro gastrointestinal digestion.

    NARCIS (Netherlands)

    Toydemir, G.; Capanoglu, E.; Kamiloglu, S.; Boyacioglu, D.; Vos, de C.H.; Hall, R.D.; Beekwilder, M.J.

    2013-01-01

    Sour cherry (Prunus cerasus L.) is rich in polyphenols, and like its processed products, is especially rich in anthocyanins. We have applied HPLC, spectrophotometric and on-line antioxidant detection methods to follow the fate of cherry antioxidants during an entire multi-step industrial-scale

  18. Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis).

    Science.gov (United States)

    Mekuria, Genet; Ramesh, Sunita A; Alberts, Evita; Bertozzi, Terry; Wirthensohn, Michelle; Collins, Graham; Sedgley, Margaret

    2003-12-01

    A technique based on the reverse transcriptase-polymerase chain reaction (RT-PCR) has been developed to detect the presence of Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) simultaneously in almond. This paper presents the results of a 3-year study comparing both enzyme-linked immunosorbent assay (ELISA) and RT-PCR for the detection of PNRSV and PDV using 175 almond leaf samples. Multiplex RT-PCR was found to be more sensitive than ELISA, especially when followed by nested PCR for the detection of PDV. The RT-PCR technique has the added advantage that plant material can be tested at any time throughout the growing season.

  19. Generic Amplicon Deep Sequencing to Determine Ilarvirus Species Diversity in Australian Prunus.

    Science.gov (United States)

    Kinoti, Wycliff M; Constable, Fiona E; Nancarrow, Narelle; Plummer, Kim M; Rodoni, Brendan

    2017-01-01

    The distribution of Ilarvirus species populations amongst 61 Australian Prunus trees was determined by next generation sequencing (NGS) of amplicons generated using a genus-based generic RT-PCR targeting a conserved region of the Ilarvirus RNA2 component that encodes the RNA dependent RNA polymerase (RdRp) gene. Presence of Ilarvirus sequences in each positive sample was further validated by Sanger sequencing of cloned amplicons of regions of each of RNA1, RNA2 and/or RNA3 that were generated by species specific PCRs and by metagenomic NGS. Prunus necrotic ringspot virus (PNRSV) was the most frequently detected Ilarvirus , occurring in 48 of the 61 Ilarvirus -positive trees and Prune dwarf virus (PDV) and Apple mosaic virus (ApMV) were detected in three trees and one tree, respectively. American plum line pattern virus (APLPV) was detected in three trees and represents the first report of APLPV detection in Australia. Two novel and distinct groups of Ilarvirus -like RNA2 amplicon sequences were also identified in several trees by the generic amplicon NGS approach. The high read depth from the amplicon NGS of the generic PCR products allowed the detection of distinct RNA2 RdRp sequence variant populations of PNRSV, PDV, ApMV, APLPV and the two novel Ilarvirus -like sequences. Mixed infections of ilarviruses were also detected in seven Prunus trees. Sanger sequencing of specific RNA1, RNA2, and/or RNA3 genome segments of each virus and total nucleic acid metagenomics NGS confirmed the presence of PNRSV, PDV, ApMV and APLPV detected by RNA2 generic amplicon NGS. However, the two novel groups of Ilarvirus -like RNA2 amplicon sequences detected by the generic amplicon NGS could not be associated to the presence of sequence from RNA1 or RNA3 genome segments or full Ilarvirus genomes, and their origin is unclear. This work highlights the sensitivity of genus-specific amplicon NGS in detection of virus sequences and their distinct populations in multiple samples, and the

  20. Generic Amplicon Deep Sequencing to Determine Ilarvirus Species Diversity in Australian Prunus

    Directory of Open Access Journals (Sweden)

    Wycliff M. Kinoti

    2017-06-01

    Full Text Available The distribution of Ilarvirus species populations amongst 61 Australian Prunus trees was determined by next generation sequencing (NGS of amplicons generated using a genus-based generic RT-PCR targeting a conserved region of the Ilarvirus RNA2 component that encodes the RNA dependent RNA polymerase (RdRp gene. Presence of Ilarvirus sequences in each positive sample was further validated by Sanger sequencing of cloned amplicons of regions of each of RNA1, RNA2 and/or RNA3 that were generated by species specific PCRs and by metagenomic NGS. Prunus necrotic ringspot virus (PNRSV was the most frequently detected Ilarvirus, occurring in 48 of the 61 Ilarvirus-positive trees and Prune dwarf virus (PDV and Apple mosaic virus (ApMV were detected in three trees and one tree, respectively. American plum line pattern virus (APLPV was detected in three trees and represents the first report of APLPV detection in Australia. Two novel and distinct groups of Ilarvirus-like RNA2 amplicon sequences were also identified in several trees by the generic amplicon NGS approach. The high read depth from the amplicon NGS of the generic PCR products allowed the detection of distinct RNA2 RdRp sequence variant populations of PNRSV, PDV, ApMV, APLPV and the two novel Ilarvirus-like sequences. Mixed infections of ilarviruses were also detected in seven Prunus trees. Sanger sequencing of specific RNA1, RNA2, and/or RNA3 genome segments of each virus and total nucleic acid metagenomics NGS confirmed the presence of PNRSV, PDV, ApMV and APLPV detected by RNA2 generic amplicon NGS. However, the two novel groups of Ilarvirus-like RNA2 amplicon sequences detected by the generic amplicon NGS could not be associated to the presence of sequence from RNA1 or RNA3 genome segments or full Ilarvirus genomes, and their origin is unclear. This work highlights the sensitivity of genus-specific amplicon NGS in detection of virus sequences and their distinct populations in multiple samples

  1. Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.

    OpenAIRE

    FAJARDO, T. V. M.; NASCIMENTO, M. B.; EIRAS, M.; NICKEL, O.; PIO-RIBEIRO, G.

    2016-01-01

    ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of...

  2. A fissitunicate ascus mechanism in the Calosphaeriaceae, and novel species of Jattaea and Calosphaeria on Prunus wood

    NARCIS (Netherlands)

    Damm, U.; Crous, P.W.; Fourie, P.H.

    2008-01-01

    During a survey of Prunus wood from South Africa, isolations were made of three presumably Calosphaerialean fungi that formed hyphomycetous, phialidic anamorphs in culture. In order to reveal the phylogenetic relationship of these fungi, they were characterised on a morphological and molecular (LSU

  3. Complete chloroplast genome of Prunus yedoensis Matsum.(Rosaceae), wild and endemic flowering cherry on Jeju Island, Korea.

    Science.gov (United States)

    Cho, Myong-Suk; Hyun Cho, Chung; Yeon Kim, Su; Su Yoon, Hwan; Kim, Seung-Chul

    2016-09-01

    The complete chloroplast genome sequences of the wild flowering cherry, Prunus yedoensis Matsum., which is native and endemic to Jeju Island, Korea, is reported in this study. The genome size is 157 786 bp in length with 36.7% GC content, which is composed of LSC region of 85 908 bp, SSC region of 19 120 bp and two IR copies of 26 379 bp each. The cp genome contains 131 genes, including 86 coding genes, 8 rRNA genes and 37 tRNA genes. The maximum likelihood analysis was conducted to verify a phylogenetic position of the newly sequenced cp genome of P. yedoensis using 11 representatives of complete cp genome sequences within the family Rosaceae. The genus Prunus exhibited monophyly and the result of the phylogenetic relationship agreed with the previous phylogenetic analyses within Rosaceae.

  4. Engineering cherry rootstocks with resistance to Prunus necrotic ring spot virus through RNAi-mediated silencing.

    Science.gov (United States)

    Song, Guo-qing; Sink, Kenneth C; Walworth, Aaron E; Cook, Meridith A; Allison, Richard F; Lang, Gregory A

    2013-08-01

    Prunus necrotic ringspot virus (PNRSV) is a major pollen-disseminated ilarvirus that adversely affects many Prunus species. In this study, an RNA interference (RNAi) vector pART27-PNRSV containing an inverted repeat (IR) region of PNRSV was transformed into two hybrid (triploid) cherry rootstocks, 'Gisela 6' (GI 148-1) and 'Gisela 7'(GI 148-8)', which are tolerant and sensitive, respectively, to PNRSV infection. One year after inoculation with PNRSV plus Prune Dwarf Virus, nontransgenic 'Gisela 6' exhibited no symptoms but a significant PNRSV titre, while the transgenic 'Gisela 6' had no symptoms and minimal PNRSV titre. The nontransgenic 'Gisela 7' trees died, while the transgenic 'Gisela 7' trees survived. These results demonstrate the RNAi strategy is useful for developing viral resistance in fruit rootstocks, and such transgenic rootstocks may have potential to enhance production of standard, nongenetically modified fruit varieties while avoiding concerns about transgene flow and exogenous protein production that are inherent for transformed fruiting genotypes. © 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

  5. Incidence of Prunus necrotic ringspot virus in Jordan

    Directory of Open Access Journals (Sweden)

    N. Salem

    2003-12-01

    Full Text Available A survey of Prunus necrotic ringspot virus (PNRSV incidence in Jordan stone-fruit growing areas was conducted during 2000–2002. A total of 2552 samples were collected from 72 commercial orchards, a mother block, 15 nurseries, and a varietal collection. A total of 208 almond, 451 apricot, 149 cherry, 250 nectarine, 1016 peach, and 478 plum trees were tested individually for PNRSV by the double-antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA. Around 15% of tested samples were infected with PNRSV. The virus incidence in almond, nectarine, plum, peach, cherry, and apricot was 24, 16, 16, 14, 13, and 10% of tested trees respectively. The level of viral infection was highest in the mother block (19%, and lowest in the samples from the nurseries (10%.

  6. Ilarviruses of Prunus spp.: a continued concern for fruit trees.

    Science.gov (United States)

    Pallas, V; Aparicio, F; Herranz, M C; Amari, K; Sanchez-Pina, M A; Myrta, A; Sanchez-Navarro, J A

    2012-12-01

    Prunus spp. are affected by a large number of viruses, causing significant economic losses through either direct or indirect damage, which results in reduced yield and fruit quality. Among these viruses, members of the genus Ilarvirus (isometric labile ringspot viruses) occupy a significant position due to their distribution worldwide. Although symptoms caused by these types of viruses were reported early in the last century, their molecular characterization was not achieved until the 1990s, much later than for other agronomically relevant viruses. This was mainly due to the characteristic liability of virus particles in tissue extracts. In addition, ilarviruses, together with Alfalfa mosaic virus, are unique among plant viruses in that they require a few molecules of the coat protein in the inoculum in order to be infectious, a phenomenon known as genome activation. Another factor that has made the study of this group of viruses difficult is that infectious clones have been obtained only for the type member of the genus, Tobacco streak virus. Four ilarviruses, Prunus necrotic ringspot virus, Prune dwarf virus, Apple mosaic virus, and American plum line pattern virus, are pathogens of the main cultivated fruit trees. As stated in the 9th Report of the International Committee on Taxonomy of Viruses, virions of this genus are "unpromising subjects for the raising of good antisera." With the advent of molecular approaches for their detection and characterization, it has been possible to get a more precise view of their prevalence and genome organization. This review updates our knowledge on the incidence, genome organization and expression, genetic diversity, modes of transmission, and diagnosis, as well as control of this peculiar group of viruses affecting fruit trees.

  7. Efeito do ácido indolbutírico no enraizamento de estacas de ramos de plantas de ameixeira (Prunus salicina, Lindl. Effect of indolbutyric acid on the rooting of plum branch cuttings (Prunus sofrena, Lindl.

    Directory of Open Access Journals (Sweden)

    E. Kersten

    1993-05-01

    Full Text Available O presente trabalho foi realizado com o objetivo de avaliar o efeito de quatro concentrações de ácido indolbutírico (IBA, no enraizamento de estacas de ramos de ameixeira (Prunus salicina, Lindl., coletadas de plantas tratadas com bórax e sulfato de zinco e controle, executado em quatro épocas. Os experimentos foram conduzidos em viveiros, sob condição de nebulização artificial intermitente. Os resultados obtidos mostram a influência de cultivar, época e concentração de IBA, não sendo verificado efeito de bórax ou sulfato de zinco na percentagem de estacas enraizadas.The objective of this work was to evaluate the effect of four concentrations of indolbutyric acid on the rooting of plum branch cuttings (Prunus salicina, Lindl. collected from plants treated with borax and zinc sulfate, in four periods. The experiments were conducted in a nursery with intermitent artificial mist conditions. The results showed a positive effect of cultivars, periods and IBA concentrations, and there was no effect of either borax or zinc sulfate on rooting of branch cuttings.

  8. Differentiation among isolates of prunus necrotic ringspot virus by transcript conformation polymorphism.

    Science.gov (United States)

    Rosner, A; Maslenin, L; Spiegel, S

    1998-09-01

    A method based on differences in electrophoretic mobility of RNA transcripts made from polymerase chain reaction (PCR) products was used for differentiation among virus isolates. A T7 RNA polymerase promoter was attached to amplified prunus necrotic ringspot virus (PNRSV) sequences by PCR. The PCR products then served as a template for transcription. Single-stranded transcripts originated from different PNRSV isolates varied in electrophoretic mobility in polyacrylamide gels, presumably because of transcript conformation polymorphism (TCP). This procedure was applied for the differentiation of PNRSV isolates.

  9. Genetic diversity of the movement and coat protein genes of South American isolates of Prunus necrotic ringspot virus.

    Science.gov (United States)

    Fiore, Nicola; Fajardo, Thor V M; Prodan, Simona; Herranz, María Carmen; Aparicio, Frederic; Montealegre, Jaime; Elena, Santiago F; Pallás, Vicente; Sánchez-Navarro, Jesús

    2008-01-01

    Prunus necrotic ringspot virus (PNRSV) is distributed worldwide, but no molecular data have been previously reported from South American isolates. The nucleotide sequences corresponding to the movement (MP) and coat (CP) proteins of 23 isolates of PNRSV from Chile, Brazil, and Uruguay, and from different Prunus species, have been obtained. Phylogenetic analysis performed with full-length MP and CP sequences from all the PNRSV isolates confirmed the clustering of the isolates into the previously reported PV32-I, PV96-II and PE5-III phylogroups. No association was found between specific sequences and host, geographic origin or symptomatology. Comparative analysis showed that both MP and CP have phylogroup-specific amino acids and all of the motifs previously characterized for both proteins. The study of the distribution of synonymous and nonsynonymous changes along both open reading frames revealed that most amino acid sites are under the effect of negative purifying selection.

  10. Interspecific variation in tree seedlings establishment in canopy gaps in relation to tree density

    Energy Technology Data Exchange (ETDEWEB)

    Reader, R.J.; Bonser, S.P.; Duralia, T.E.; Bricker, B.D. [Guelph Univ., ON (Canada). Dept. of Botany

    1995-10-01

    We tested whether interspecific variation in tree seedling establishment in canopy gaps was significantly related to interspecific variation in tree density, for seven deciduous forest tree species (Quercus alba, Hamamelis virginiana, Acer rubrum, Sassafras albidum, Quercus rubra, Prunus serotina, Ostrya virginiana). For each species, seedling establishment was calculated as the difference in seedling density before experimental gap creation versus three years after gap creation. In each of the six experimentally-created gap types (33% or 66% removal of tree basal area from 0.01ha, 0.05ha or 0.20ha patches), differences in seedling establishment among species were significantly related to differences in their density in the tree canopy. A regression model with log{sub e} tree density as the independent variable accounted for between 93% and 98% of interspecific variation in seedling establishment. Our results provide empirical support for models of tree dynamics in gaps that assume seedling establishment depends on canopy tree density. 17 refs, 1 fig, 3 tabs

  11. Composition and antioxidant properties of fresh and frozen stored blackthorn fruits (Prunus spinosa L.)

    OpenAIRE

    Elżbieta Sikora; Małgorzata I. Bieniek; Barbara Borczak

    2013-01-01

      Aim. Blackthorn (Prunus spinosa L.) is quite widespread bush occurring in Poland. Its fruits are easily available food products. The aim of this study was to investigate the basal chemical composition, especially antioxidant compounds in fresh and frozen stored blackthorn fruits. Material  and methods. Research material consisted of blackthorn fruits collected from the wild grown bushes, near Łącko. In the wash-out, dried and stone-loss fruits, the content of dry matter, protein, ...

  12. Cultural Resources Survey and Testing Along Ditch 19 and Extensive Testing of 23DU289, Dunklin and Stoddard Counties, Missouri.

    Science.gov (United States)

    1988-06-30

    Carya illinoensis ) 1 1 Persimmon (Diospyros virginiana) T 2 2 Pl.um (Prunus sp.) T Red Haw (Crataegus sp.) T 1 11 Red Mulberry (Morus rubra) T...2 Cherry (Prunus sp.) T Cottonwood (Populus sp.) 1 3 Dogwood (Cornus sp.) 1 Hackberry (Celtus occidentalis) 12 9 Hickory, ( Carya sp.) 5 4 Shellhark... Carya laciniosa) T Hornbeam (Ostrya virginiana) 2 Kentucky Coffee Tree( Gymnoeladus dioica)T Locust, T Black (Robinia pseudo-acacia) T Honey

  13. Resistance to Plum Pox Virus (PPV) in apricot (Prunus armeniaca L.) is associated with down-regulation of two MATHd genes.

    Science.gov (United States)

    Zuriaga, Elena; Romero, Carlos; Blanca, Jose Miguel; Badenes, Maria Luisa

    2018-01-27

    Plum pox virus (PPV), causing Sharka disease, is one of the main limiting factors for Prunus production worldwide. In apricot (Prunus armeniaca L.) the major PPV resistance locus (PPVres), comprising ~ 196 kb, has been mapped to the upper part of linkage group 1. Within the PPVres, 68 genomic variants linked in coupling to PPV resistance were identified within 23 predicted transcripts according to peach genome annotation. Taking into account the predicted functions inferred from sequence homology, some members of a cluster of meprin and TRAF-C homology domain (MATHd)-containing genes were pointed as PPV resistance candidate genes. Here, we have characterized the global apricot transcriptome response to PPV-D infection identifying six PPVres locus genes (ParP-1 to ParP-6) differentially expressed in resistant/susceptible cultivars. Two of them (ParP-3 and ParP-4), that encode MATHd proteins, appear clearly down-regulated in resistant cultivars, as confirmed by qRT-PCR. Concurrently, variant calling was performed using whole-genome sequencing data of 24 apricot cultivars (10 PPV-resistant and 14 PPV-susceptible) and 2 wild relatives (PPV-susceptible). ParP-3 and ParP-4, named as Prunus armeniaca PPVres MATHd-containing genes (ParPMC), are the only 2 genes having allelic variants linked in coupling to PPV resistance. ParPMC1 has 1 nsSNP, while ParPMC2 has 15 variants, including a 5-bp deletion within the second exon that produces a frameshift mutation. ParPMC1 and ParPMC2 are adjacent and highly homologous (87.5% identity) suggesting they are paralogs originated from a tandem duplication. Cultivars carrying the ParPMC2 resistant (mutated) allele show lack of expression in both ParPMC2 and especially ParPMC1. Accordingly, we hypothesize that ParPMC2 is a pseudogene that mediates down-regulation of its functional paralog ParPMC1 by silencing. As a whole, results strongly support ParPMC1 and/or ParPMC2 as host susceptibility genes required for PPV infection which

  14. Virulence and molecular polymorphism of Prunus necrotic ringspot virus isolates.

    Science.gov (United States)

    Hammond, R W; Crosslin, J M

    1998-07-01

    Prunus necrotic ringspot virus (PNRSV) occurs as numerous strains or isolates that vary widely in their pathogenic, biophysical and serological properties. Prior attempts to distinguish pathotypes based upon physical properties have not been successful; our approach was to examine the molecular properties that may distinguish these isolates. The nucleic acid sequence was determined from 1.65 kbp RT-PCR products derived from RNA 3 of seven distinct isolates of PNRSV that differ serologically and in pathology on sweet cherry. Sequence comparisons of ORF 3a (putative movement protein) and ORF 3b (coat protein) revealed single nucleotide and amino acid differences with strong correlations to serology and symptom types (pathotypes). Sequence differences between serotypes and pathotypes were also reflected in the overall phylogenetic relationships between the isolates.

  15. Variability and molecular typing of the woody-tree infecting prunus necrotic ringspot ilarvirus.

    Science.gov (United States)

    Vasková, D; Petrzik, K; Karesová, R

    2000-01-01

    The 3'-part of the movement protein gene, the intergenic region and the complete coat protein gene of sixteen isolates of Prunus necrotic ringspot virus (PNRSV) from five different host species from the Czech Republic were sequenced in order to search for the bases of extensive variability of viroses caused by this pathogen. According to phylogenetic analyses all the 46 isolates sequenced to date split into three main groups, which correlated to a certain extend with their geographic origin. Modelled serological properties showed that all the new isolates belong to one serotype.

  16. Propagation of the endangered Azorean cherry Prunus azorica using stem cuttings and air layering

    OpenAIRE

    Moreira, Orlanda; Martins, José; Silva, Luís; Moura, Mónica

    2009-01-01

    Prunus azorica (Hort. ex Mouillef.) Rivas Mart., Lousã, Fern. Prieto, E. Dias, J.C. Costa & C. Aguiar is an endangered tree endemic to the Azores, with an ecological and ornamental interest. The objective of this study was to determine the conditions necessary for the successful propagation of P. azorica by stem cuttings and air-layering. Stem cuttings collected in March with two apical leaf pairs pruned to 1/3 of their leaf area were submitted to different treatments, including a basal split...

  17. Novel Prunus rootstock somaclonal variants with divergent ability to tolerate waterlogging.

    Science.gov (United States)

    Pistelli, Laura; Iacona, Calogero; Miano, Dario; Cirilli, Marco; Colao, Maria Chiara; Mensuali-Sodi, Anna; Muleo, Rosario

    2012-03-01

    Plants require access to free water for nutrient uptake, but excess water surrounding the roots can be injurious or even lethal because it blocks the transfer of free oxygen between the soil and the atmosphere. Genetic improvement efforts in this study were focused on the increased tolerance in roots to waterlogging. Among a pool of clones generated in vitro from leaf explants of rootstock Mr.S.2/5 of Prunus cerasifera L., the S.4 clone was flood tolerant whereas the S.1 clone was sensitive. The S.4 clone formed adventitious roots on exposure to flooding. Moreover, the chlorophyll content and mitochondrial activity in the leaf and root, soluble sugar content, alcohol dehydrogenase activity and ethylene content were different between the clones. The sorbitol transporter gene (SOT1) was up-regulated during hypoxia, the alcohol dehydrogenase genes (ADH1 and ADH3) were up-regulated in the leaves and down-regulated in the roots of the S.4 clone during hypoxia, and the 1-aminocyclopropane-1-oxidase gene (ACO1) was up-regulated in the leaves and roots of the S.4 clone during hypoxia and down-regulated in the wild-type roots. In addition, in the S.4 root, hypoxia induced significant down-regulation of a glycosyltransferase-like gene (GTL), which has a yet-undefined role. Although the relevant variation in the S.4 genome has yet to be determined, genetic alteration clearly conferred a flooding-tolerant phenotype. The isolation of novel somaclonals with the same genomic background but with divergent tolerance to flooding may offer new insights in the elucidation of the genetic machinery of resistance to flooding and aid in the selection of new Prunus rootstocks to be used in various adverse environments.

  18. Wintercuring of Prunus dulcis cv ‘Butte,’ P. webbii and their interspecific hybrid in response to Xylella fastidiosa infections

    Science.gov (United States)

    Clonal replicates of Prunus dulcis cv ‘Butte,’ P. webbii and their interspecific hybrid P 63-61 were inoculated with Xylella fastidiosa strain M23 and evaluated for Almond Leaf Scorch Disease and subsequent wintercuring of infections during three growing seasons. Initial inoculations established gr...

  19. Microclonal Multiplication of wild Cherry (Prunus avium L.) from Shoot Tips and Root Sucker Buds

    OpenAIRE

    Pevalek-Kozlina, Branka; Michler, Charles H.; Jelaska, Sibila

    1994-01-01

    The effects of different combinations and concentrations of the growth regulators: 6-benzylaminopurine (BA), 6-furfurylaminopurine (KIN), N6- (2-isopentenyl) adenine (2iP), indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and a-naphthaleneacetic acid (NAA) on axillary shoot multiplication rates for wild cherry (Prunus avium L.) shoot explants were determined. Apical shoot tips and axillary buds from juvenile trees (5-year old) and from root suckers of mature trees (55-year old) were us...

  20. An in situ, seasonal study of volatiles from a single cultivar of Prunus dulcis, and their relationship to navel orangeworm moth

    Science.gov (United States)

    Nonpareil almonds, Prunus dulcis, account for the largest percentage of almond varieties grown in the Central and San Joaquin valleys of California. Several studies have investigated the various non-volatile and volatile components of various plant parts; however, the volatile organic compound (VOC)...

  1. Characterization of New Isolates of Apricot vein clearing-associated virus and of a New Prunus-Infecting Virus: Evidence for Recombination as a Driving Force in Betaflexiviridae Evolution.

    Directory of Open Access Journals (Sweden)

    Armelle Marais

    Full Text Available Double stranded RNAs from Prunus samples gathered from various surveys were analyzed by a deep-sequencing approach. Contig annotations revealed the presence of a potential new viral species in an Azerbaijani almond tree (Prunus amygdalus and its genome sequence was completed. Its genomic organization is similar to that of the recently described Apricot vein clearing associated virus (AVCaV for which two new isolates were also characterized, in a similar fashion, from two Japanese plums (Prunus salicina from a French germplasm collection. The amino acid identity values between the four proteins encoded by the genome of the new virus have identity levels with those of AVCaV which fall clearly outside the species demarcation criteria. The new virus should therefore be considered as a new species for which the name of Caucasus prunus virus (CPrV has been proposed. Phylogenetic relationships and nucleotide comparisons suggested that together with AVCaV, CPrV could define a new genus (proposed name: Prunevirus in the family Betaflexiviridae. A molecular test targeting both members of the new genus was developed, allowing the detection of additional AVCaV isolates, and therefore extending the known geographical distribution and the host range of AVCaV. Moreover, the phylogenetic trees reconstructed with the amino acid sequences of replicase, movement and coat proteins of representative Betaflexiviridae members suggest that Citrus leaf blotch virus (CLBV, type member of the genus Citrivirus may have evolved from a recombination event involving a Prunevirus, further highlighting the importance of recombination as a driving force in Betaflexiviridae evolution. The sequences reported in the present manuscript have been deposited in the GenBank database under accession numbers KM507061-KM504070.

  2. Seed washing, exogenous application of gibberellic acid, and cold stratification enhance the germination of sweet cherry (Prunus avium L.) seed

    NARCIS (Netherlands)

    Javanmard, T.; Zamani, Z.; Keshavarz Afshar, R.; Hashemi, M.; Struik, P.C.

    2014-01-01

    Seed germination in sweet cherry (Prunus avium L.) is a slow and lengthy process which has delayed breeding efforts. In this study, seed from ripe fruit of the sweet cherry cultivar ‘Lambert’ were collected and, after removing the endocarp, various dormancy-breaking treatments such as seed washing,

  3. Analysis of intra-host genetic diversity of Prunus necrotic ringspot virus (PNRSV) using amplicon next generation sequencing.

    Science.gov (United States)

    Kinoti, Wycliff M; Constable, Fiona E; Nancarrow, Narelle; Plummer, Kim M; Rodoni, Brendan

    2017-01-01

    PCR amplicon next generation sequencing (NGS) analysis offers a broadly applicable and targeted approach to detect populations of both high- or low-frequency virus variants in one or more plant samples. In this study, amplicon NGS was used to explore the diversity of the tripartite genome virus, Prunus necrotic ringspot virus (PNRSV) from 53 PNRSV-infected trees using amplicons from conserved gene regions of each of PNRSV RNA1, RNA2 and RNA3. Sequencing of the amplicons from 53 PNRSV-infected trees revealed differing levels of polymorphism across the three different components of the PNRSV genome with a total number of 5040, 2083 and 5486 sequence variants observed for RNA1, RNA2 and RNA3 respectively. The RNA2 had the lowest diversity of sequences compared to RNA1 and RNA3, reflecting the lack of flexibility tolerated by the replicase gene that is encoded by this RNA component. Distinct PNRSV phylo-groups, consisting of closely related clusters of sequence variants, were observed in each of PNRSV RNA1, RNA2 and RNA3. Most plant samples had a single phylo-group for each RNA component. Haplotype network analysis showed that smaller clusters of PNRSV sequence variants were genetically connected to the largest sequence variant cluster within a phylo-group of each RNA component. Some plant samples had sequence variants occurring in multiple PNRSV phylo-groups in at least one of each RNA and these phylo-groups formed distinct clades that represent PNRSV genetic strains. Variants within the same phylo-group of each Prunus plant sample had ≥97% similarity and phylo-groups within a Prunus plant sample and between samples had less ≤97% similarity. Based on the analysis of diversity, a definition of a PNRSV genetic strain was proposed. The proposed definition was applied to determine the number of PNRSV genetic strains in each of the plant samples and the complexity in defining genetic strains in multipartite genome viruses was explored.

  4. Analysis of intra-host genetic diversity of Prunus necrotic ringspot virus (PNRSV using amplicon next generation sequencing.

    Directory of Open Access Journals (Sweden)

    Wycliff M Kinoti

    Full Text Available PCR amplicon next generation sequencing (NGS analysis offers a broadly applicable and targeted approach to detect populations of both high- or low-frequency virus variants in one or more plant samples. In this study, amplicon NGS was used to explore the diversity of the tripartite genome virus, Prunus necrotic ringspot virus (PNRSV from 53 PNRSV-infected trees using amplicons from conserved gene regions of each of PNRSV RNA1, RNA2 and RNA3. Sequencing of the amplicons from 53 PNRSV-infected trees revealed differing levels of polymorphism across the three different components of the PNRSV genome with a total number of 5040, 2083 and 5486 sequence variants observed for RNA1, RNA2 and RNA3 respectively. The RNA2 had the lowest diversity of sequences compared to RNA1 and RNA3, reflecting the lack of flexibility tolerated by the replicase gene that is encoded by this RNA component. Distinct PNRSV phylo-groups, consisting of closely related clusters of sequence variants, were observed in each of PNRSV RNA1, RNA2 and RNA3. Most plant samples had a single phylo-group for each RNA component. Haplotype network analysis showed that smaller clusters of PNRSV sequence variants were genetically connected to the largest sequence variant cluster within a phylo-group of each RNA component. Some plant samples had sequence variants occurring in multiple PNRSV phylo-groups in at least one of each RNA and these phylo-groups formed distinct clades that represent PNRSV genetic strains. Variants within the same phylo-group of each Prunus plant sample had ≥97% similarity and phylo-groups within a Prunus plant sample and between samples had less ≤97% similarity. Based on the analysis of diversity, a definition of a PNRSV genetic strain was proposed. The proposed definition was applied to determine the number of PNRSV genetic strains in each of the plant samples and the complexity in defining genetic strains in multipartite genome viruses was explored.

  5. Radiation induced cerebellum impairments in Swiss albino mice and its modulation by dietary Prunus domestica

    International Nuclear Information System (INIS)

    Sharma, Garima; Sisodia, Rashmi

    2012-01-01

    To study the biochemical, quantitative histopathological and behavioural changes after 5 Gy whole body irradiation and its modulation by supplementation of Prunus domestica extract (PDE) for 15 consecutive days on male Swiss albino. For this study healthy mice from an inbred colony were divided into five groups: (i) Control; (ii) PDE treated - mice in this group were orally supplemented with PDE (400 mg/kg body weight (bw)/day) once daily for 15 consecutive days; (iii) Irradiated-mice were whole body exposed to 5 Gy irradiated; (iv) PDE + irradiated-mice in this group were orally supplemented PDE for 15 days (once a day) prior to irradiation; and (v) irradiated+PDE -mice in this group were administered PDE orally for 15 days (once a day) consequently after irradiation. Marked radiation induced changes in the amount of cerebellar lipid peroxidation (LPO), glutathione (GSH), protein, superoxide dismutase (SOD), catalase and histopathological changes (molecular layer, granular layer and purkinje cell numbers) could be significantly ameliorated supplementation of PDE prior/post irradiation. Radiation induced deficits in learning and memory were also significantly ameliorated. PDE was found to have strong radical scavenging activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and also showed in vitro radioprotective activity. The result of present study showed that prior/post-supplementation of Prunus domestica has radioprotective potential as well as neuroprotective properties against the radiation. (author)

  6. Food and biomass potential of Prunus virginiana L. (chokecherry).

    Science.gov (United States)

    Wang, Sunmin; Young, Lester; Faye, Amberly; Li, Bonnie; Clancy, Johanna; Bors, Bob; Reaney, Martin

    2012-03-14

    Prunus virginiana L. (chokecherry) fruit has potential to provide both food and energy and as annual yield of biomass and energy are much greater than annual crops such as canola and wheat. We determined chokecherry fruit weight fractions as well as pit and extracted seed oil concentrations and fatty acid composition. Gross energy for each of the fractions was determined, as were carbon and nitrogen content. Extrapolation of these data suggests that gross energy from pits alone over a 24-year period (890 GJ·ha(-1)) is equivalent to that from an entire canola/wheat rotation (850 GJ·ha(-1)). After maturity, pulp contributes an additional 1130 GJ·ha(-1) over 21 years from ~3.4 t·ha(-1)·year(-1) (dw), while wood from pruning could add another 60 GJ·ha(-1)·year(-1). Over this time period, chokecherry would produce 1.5-2.5 times the amount of oil produced by a canola/wheat rotation.

  7. Pomological and technological characteristics of collected selections of cherry plum Prunus cerasifera Erhr.

    Directory of Open Access Journals (Sweden)

    Miletić Rade

    2005-01-01

    Full Text Available A plantation collection containing 32 genotypes selected from spontaneous populations of cherry plum Prunus cerasifera Erhr. was set up in the region of the Eastern Serbian town of Svrljig. The fruit trees budded from Prunus cerasifera seedlings and were planted at 5x4 m spacing on a mild slope of south-western aspect. This study shows the most important characteristics of the 19 selections in the collection, and the average results recorded in the 2000-2003 period. The most significant characteristics of the trees, their productivity, and fruit and stone characteristics are presented. The average coarseness of fruits, i.e. their length, width and thickness, measured 25.0x 24.4x25.0 mm, while stone coarseness was 14.4x10.3x3.6 mm. The average fruit weight was 12.1 g (24.3-4.8 g, and stone weight 0.85 g (2.2-0.3 g. Depending on fruit and stone weight, the mesocarp content was 93% (96.3-90.3%. Taking into consideration the possibility of fruit exploitation for the production of biologically high-quality food, the mesocarp chemical composition was thoroughly examined. The fruits were found to have increased contents of total acids, achieving an average of 3.09% (3.44-2.60%, which was the initial objective of this selection. Total solids content was 13.5% (16.2-10.3%, total soluble solids 12.5% (14.5-9.5% and total sugars 6.00% (11.45-3.14%. Considering these characteristics, the selections that were singled out deserve more attention in terms of preserving their biodiversity, forming a gene bank and commercial cultivation.

  8. Using Perls Staining to Trace the Iron Uptake Pathway in Leaves of a Prunus Rootstock Treated with Iron Foliar Fertilizers.

    Science.gov (United States)

    Rios, Juan J; Carrasco-Gil, Sandra; Abadía, Anunciación; Abadía, Javier

    2016-01-01

    The aim of this study was to trace the Fe uptake pathway in leaves of Prunus rootstock (GF 677; Prunus dulcis × Prunus persica) plants treated with foliar Fe compounds using the Perls blue method, which detects labile Fe pools. Young expanded leaves of Fe-deficient plants grown in nutrient solution were treated with Fe-compounds using a brush. Iron compounds used were the ferrous salt FeSO4, the ferric salts Fe2(SO4)3 and FeCl3, and the chelate Fe(III)-EDTA, all of them at concentrations of 9 mM Fe. Leaf Fe concentration increases were measured at 30, 60, 90 min, and 24 h, and 70 μm-thick leaf transversal sections were obtained with a vibrating microtome and stained with Perls blue. In vitro results show that the Perls blue method is a good tool to trace the Fe uptake pathway in leaves when using Fe salts, but is not sensitive enough when using synthetic Fe(III)-chelates such as Fe(III)-EDTA and Fe(III)-IDHA. Foliar Fe fertilization increased leaf Fe concentrations with all Fe compounds used, with inorganic Fe salts causing larger leaf Fe concentration increases than Fe(III)-EDTA. Results show that Perls blue stain appeared within 30 min in the stomatal areas, indicating that Fe applied as inorganic salts was taken up rapidly via stomata. In the case of using FeSO4 a progression of the stain was seen with time toward vascular areas in the leaf blade and the central vein, whereas in the case of Fe(III) salts the stain mainly remained in the stomatal areas. Perls stain was never observed in the mesophyll areas, possibly due to the low concentration of labile Fe pools.

  9. In silico and experimental evaluation of DNA-based detection methods for the ability to discriminate almond from other Prunus spp.

    Science.gov (United States)

    Brežná, Barbara; Šmíd, Jiří; Costa, Joana; Radvanszky, Jan; Mafra, Isabel; Kuchta, Tomáš

    2015-04-01

    Ten published DNA-based analytical methods aiming at detecting material of almond (Prunus dulcis) were in silico evaluated for potential cross-reactivity with other stone fruits (Prunus spp.), including peach, apricot, plum, cherry, sour cherry and Sargent cherry. For most assays, the analysis of nucleotide databases suggested none or insufficient discrimination of at least some stone fruits. On the other hand, the assay targeting non-specific lipid transfer protein (Röder et al., 2011, Anal Chim Acta 685:74-83) was sufficiently discriminative, judging from nucleotide alignments. Empirical evaluation was performed for three of the published methods, one modification of a commercial kit (SureFood allergen almond) and one attempted novel method targeting thaumatin-like protein gene. Samples of leaves and kernels were used in the experiments. The empirical results were favourable for the method from Röder et al. (2011) and a modification of SureFood allergen almond kit, both showing cross-reactivity <10(-3) compared to the model almond. Copyright © 2014 Elsevier Ltd. All rights reserved.

  10. Studies on induced mutation in maitres (Prunus mume Sieb et. Zucc) through irradiation

    International Nuclear Information System (INIS)

    Niu Chuantang; He Daoyi; Li Yazhi

    1995-01-01

    Five varieties of maitre were treated with different doses of 60 Co γ-ray, then grafted to stock (Prunus armeniaca L.). Survival rate of grafting reduced with the increase of the doses. Optimum level of dose is 20∼30 Gy. At base of grafted seedling from scion woods irradiated by 30 Gy, a mutant shoot was found. the mutant has shorter inter-node than mother plant and lance-shaped leaves. All morphological characters and colour of flower of the mutant are not significantly different from mother plant. It is shown that the mutant differs from it's mother plant in leaf microstructure and on biochemistry level

  11. Post-storage cell wall metabolism in two sweet cherry (Prunus avium L.) cultivars displaying different postharvest performance

    OpenAIRE

    Belge, Burcu; Comabella, Eva; Graell i Sarle, Jordi; Lara Ayala, Isabel

    2015-01-01

    The biochemical processes underlying firmness loss of sweet cherry (Prunus avium L.) fruit are poorly understood.Studies on cell wall metabolism of sweet cherry have been generally undertaken during on-tree development or at harvest maturity, while published reports on postharvest changes are scarce and fragmentary. In this work, cell wall modifications after storage at 0 ºC were studied in two cherry cultivars ('Celeste' and 'Somerset') displaying different postharvest potential. Firmness wa...

  12. Authenticity assessment of gamma- and delta-decalactone from prunus fruits by gas chromatography combustion/pyrolysis isotope ratio mass spectrometry (GC-C/P-IRMS).

    Science.gov (United States)

    Tamura, Hirotoshi; Appel, Markus; Richling, Elke; Schreier, Peter

    2005-06-29

    Authenticity assessment of gamma-decalactone (1) and delta-decalactone (2) from peach (Prunus persica var. persica), apricot (Prunus armeniaca), and nectarine (Prunus persica var. nectarina) was performed using gas chromatography-isotope ratio mass spectrometry (GC-IRMS) in the combustion (C) and pyrolysis (P) mode. In addition, commercially available synthetic (nature-identical) 1 and 2 as well as biotechnologically produced samples (declared to be "natural") were characterized by their delta(2)H(V)(-)(SMOW) and delta(13)C(V)(-)(PDB) values. For the Prunus fruits under study, rather narrow ranges of delta(13)C(V)(-)(PDB) and delta(2)H(V)(-)(SMOW) data of 1, varying from - 34.6 per thousand to - 38.4 per thousand and -160 per thousand to -206 per thousand, respectively, were obtained. Synthetic references of 1 showed delta(13)C(V)(-)(PDB) and delta(2)H(V)(-)(SMOW) data ranging from -27.4 per thousand to -28.3 per thousand and -151 per thousand to -184 per thousand, respectively. Samples of 1 declared to be "natural" exhibited ranges from -28.1 per thousand to -29.2 per thousand and -192 per thousand to -286 per thousand for delta(13)C(V)(-)(PDB) and delta(2)H(V)(-)(SMOW), respectively. For 2 from peach, apricot, and nectarine, delta(13)C(V)(-)(PDB) values ranging from -34.0 per thousand to -37.9 per thousand were determined; the delta(2)H(V)(-)(SMOW) values ranged from -171 per thousand to -228 per thousand. The delta(13)C(V)(-)(PDB) and delta(2)H(V)(-)(SMOW) data for synthetic 2 were -28.2 per thousand and -171 per thousand, respectively, that is, similar to those of 2 from "natural" origin, ranging from -27.7 per thousand to -30.1 per thousand and -185 per thousand to -230 per thousand for delta(13)C(V)(-)(PDB) and delta(2)H(V)(-)(SMOW), respectively. GC-C/P-IRMS allowed clear-cut analytical differentiation of the synthetic and "ex-plant" origin of 1 and 2, whereas narrow ranges of delta(13)C(V)(-)(PDB) and delta(2)H(V)(-)(SMOW) data were found for samples of

  13. A Forest Tent Caterpillar Outbreak Increased Resource Levels and Seedling Growth in a Northern Hardwood Forest.

    Directory of Open Access Journals (Sweden)

    Danaë M A Rozendaal

    Full Text Available In closed-canopy forests, gap formation and closure are thought to be major drivers of forest dynamics. Crown defoliation by insects, however, may also influence understory resource levels and thus forest dynamics. We evaluate the effect of a forest tent caterpillar outbreak on understory light availability, soil nutrient levels and tree seedling height growth in six sites with contrasting levels of canopy defoliation in a hardwood forest in northern lower Michigan. We compared resource levels and seedling growth of six hardwood species before, during and in the three years after the outbreak (2008-2012. Canopy openness increased strongly during the forest tent caterpillar outbreak in the four moderately and severely defoliated sites, but not in lightly defoliated sites. Total inorganic soil nitrogen concentrations increased in response to the outbreak in moderately and severely defoliated sites. The increase in total inorganic soil nitrogen was driven by a strong increase in soil nitrate, and tended to become stronger with increasing site defoliation. Seedling height growth increased for all species in the moderately and severely defoliated sites, but not in lightly defoliated sites, either during the outbreak year or in the year after the outbreak. Growth increases did not become stronger with increasing site defoliation, but were strongest in a moderately defoliated site with high soil nutrient levels. Growth increases tended to be strongest for the shade intolerant species Fraxinus americana and Prunus serotina, and the shade tolerant species Ostrya virginiana. The strong growth response of F. americana and P. serotina suggests that recurring forest tent caterpillar outbreaks may facilitate the persistence of shade intolerant species in the understory in the absence of canopy gaps. Overall, our results suggest that recurrent canopy defoliation resulting from cyclical forest insect outbreaks may be an additional driver of dynamics in

  14. A Forest Tent Caterpillar Outbreak Increased Resource Levels and Seedling Growth in a Northern Hardwood Forest.

    Science.gov (United States)

    Rozendaal, Danaë M A; Kobe, Richard K

    2016-01-01

    In closed-canopy forests, gap formation and closure are thought to be major drivers of forest dynamics. Crown defoliation by insects, however, may also influence understory resource levels and thus forest dynamics. We evaluate the effect of a forest tent caterpillar outbreak on understory light availability, soil nutrient levels and tree seedling height growth in six sites with contrasting levels of canopy defoliation in a hardwood forest in northern lower Michigan. We compared resource levels and seedling growth of six hardwood species before, during and in the three years after the outbreak (2008-2012). Canopy openness increased strongly during the forest tent caterpillar outbreak in the four moderately and severely defoliated sites, but not in lightly defoliated sites. Total inorganic soil nitrogen concentrations increased in response to the outbreak in moderately and severely defoliated sites. The increase in total inorganic soil nitrogen was driven by a strong increase in soil nitrate, and tended to become stronger with increasing site defoliation. Seedling height growth increased for all species in the moderately and severely defoliated sites, but not in lightly defoliated sites, either during the outbreak year or in the year after the outbreak. Growth increases did not become stronger with increasing site defoliation, but were strongest in a moderately defoliated site with high soil nutrient levels. Growth increases tended to be strongest for the shade intolerant species Fraxinus americana and Prunus serotina, and the shade tolerant species Ostrya virginiana. The strong growth response of F. americana and P. serotina suggests that recurring forest tent caterpillar outbreaks may facilitate the persistence of shade intolerant species in the understory in the absence of canopy gaps. Overall, our results suggest that recurrent canopy defoliation resulting from cyclical forest insect outbreaks may be an additional driver of dynamics in temperate closed

  15. Genotyping by sequencing for SNP-based linkage analysis and identification of QTLs linked to fruit quality traits in Japanese plum (Prunus salicina Lindl.)

    Science.gov (United States)

    Marker-assisted selection (MAS) in stone fruit (Prunus species) breeding is currently difficult to achieve due to the polygenic nature of themost relevant agronomic traits linked to fruit quality. Genotyping by sequencing (GBS), however, provides a large quantity of useful data suitable for finemapp...

  16. Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

    Directory of Open Access Journals (Sweden)

    Thor Vinícius Martins Fajardo

    2015-12-01

    Full Text Available ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV, except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP and coat (CP protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

  17. Investigation of the aroma of commercial peach (Prunus persica L. Batsch) types by Proton Transfer Reaction-Mass Spectrometry (PTR-MS) and sensory analysis

    NARCIS (Netherlands)

    Cardoso Ferreira Pinhancos de Bianchi, Tiago; Weesepoel, Yannick; Koot, Alex; Iglesias, Ignasi; Eduardo, Iban; Gratacós-Cubarsí, Marta; Guerrero, Luis; Hortós, Maria; Ruth, van Saskia

    2017-01-01

    The aim of this study was to investigate the aroma and sensory profiles of various types of peaches (Prunus persica L. Batsch.). Forty-three commercial cultivars comprising peaches, flat peaches, nectarines, and canning peaches (pavías) were grown over two consecutive harvest years. Fruits were

  18. Black to Black

    DEFF Research Database (Denmark)

    Langkjær, Michael Alexander

    2012-01-01

    Pop musicians performing in black stage costume take advantage of cultural traditions relating to matters black. Stylistically, black is a paradoxical color: although a symbol of melancholy, pessimism, and renunciation, black also expresses minimalist modernity and signifies exclusivity (as is hi...

  19. Plum pox virus accumulates mutations in different genome parts during a long-term maintenance in Prunus host plants and passage in Nicotiana benthamiana.

    Science.gov (United States)

    Vozárová, Z; Kamencayová, M; Glasa, M; Subr, Z

    2013-01-01

    Plum pox virus (PPV) isolates of the strain PPV-M prevalently infect peaches under natural conditions in Middle Europe. Comparison of complete genome sequences obtained from subisolates of a PPV-M isolate maintained experimentally over a 6-year period in different Prunus host species and passaged in Nicotiana benthamiana was performed with the aim to highlight the mutations potentially connected with the virus-host adaptation. The results showed that the lowest number of non-silent mutations was accumulated in PPV-M maintained in peach (original host species), approximately two times higher diversity was recorded in plum, apricot and N. benthamiana, indicating the genetic determination of the PPV host preference. The sequence variability of Prunus subisolates was distributed more or less evenly along the PPV genome and no amino acid motif could be outlined as responsible for the host adaptation. In N. benthamiana the mutations were accumulated notably in the P1 and P3 genes indicating their non-essentiality in the infection of this experimental host plant.

  20. Propagation and Growth of Chokecherry (Prunus virginaiana) as an Alternative, Water-wise, Fruit Crop for the Intermountain West

    OpenAIRE

    Crook, Jeremy R.

    2010-01-01

    Utah fruit growers have shown interest in chokecherry (Prunus virginiana) as an alternative crop that has low requirements for water and soil fertility. Consumers want native fruits like chokecherry that are healthy and taste good. Currently, the limiting factor in developing a chokecherry industry in Utah is the ability to propagate large numbers of plants for orchard establishment. Chokecherries are difficult to propagate by traditional means because of their low rooting percentages. Plant ...

  1. Molecular characterization of two prunus necrotic ringspot virus isolates from Canada.

    Science.gov (United States)

    Cui, Hongguang; Hong, Ni; Wang, Guoping; Wang, Aiming

    2012-05-01

    We determined the entire RNA1, 2 and 3 sequences of two prunus necrotic ringspot virus (PNRSV) isolates, Chr3 from cherry and Pch12 from peach, obtained from an orchard in the Niagara Fruit Belt, Canada. The RNA1, 2 and 3 of the two isolates share nucleotide sequence identities of 98.6%, 98.4% and 94.5%, respectively. Their RNA1- and 2-encoded amino acid sequences are about 98% identical to the corresponding sequences of a cherry isolate, CH57, the only other PNRSV isolate with complete RNA1 and 2 sequences available. Phylogenetic analysis of the coat protein and movement protein encoded by RNA3 of Pch12 and Chr3 and published PNRSV isolates indicated that Chr3 belongs to the PV96 group and Pch12 belongs to the PV32 group.

  2. EVALUATION OF GAS EXCHANGES IN DIFFERENT Prunus SPP. ROOTSTOCKS UNDER DROUGHT AND FLOODING STRESS

    Directory of Open Access Journals (Sweden)

    ELSA KUHN KLUMB

    2017-10-01

    Full Text Available ABSTRACT The state of Rio Grande do Sul is the largest peach productor in Brazil; however, it still possesses poor yield values when compared with other states. One of the problems associated with this is the occurrence of soils with drainage problems, mainly in Pelotas region, which depending on the year period, may undergo water deficit or flooding situations in the great majority of the years, which harm the crop development and yield. Among the harmful effects caused by these stresses stand out, the decrease in the net assimilation rate, closure of stomata, reduction of the cell activities, production of reactive oxygen species, membrane and protein destabilization. Thus, the aim of this study was to investigate in what magnitude of the gaseous exchange parameters of Prunus spp. rootstocks are influenced under drought and flood stress. In the experiment, gas exchange parameters net photosynthetic rate (A stomata conductance (gs, intercellular carbon (Ci and transpiration (E] were evaluated in three Prunus spp. rootstocks (peach tree ‘Capdeboscq’ and plum trees ‘Julior’ and ‘Marianna 2624’ under three water conditions (control, water deficit and soil flooding for seven days. The three rootstocks proved more susceptible to flooding than to water deficit, only varying in response time, which is intrinsic to each genotype, and that there is a genetic variability for the tolerance to the studied stresses. The variation on physiological response to the water deficit stress was later in both evaluated genotypes. However, in general, ‘Julior’ presented greater tolerance to both stresses when compared to the other rootstocks evaluated. Such information is useful to help in the choice of rootstocks for plant production, in the orchard management and for plant breeding programs, aiming at the selection of new genotypes with increased tolerance to these water stresses.

  3. Vegetative and seedling regeneration of pin cherry (Prunus pensylvanica): Efficacy of herbicide treatment. NODA note No. 21

    Energy Technology Data Exchange (ETDEWEB)

    Mallik, A U; Bell, F W; Peterson, G W

    1996-11-01

    Pin cherry (Prunus pensylvanica L.) is a major competing plant commonly found in young conifer plantations in both boreal and northern hardwood forests. This note describes and presents results of a study conducted to determine, for pin cherry, the ratio of the current year`s seedling recruitment versus the previous year`s stem density; seed production; the soil seed bank; and the efficacy of a glyphosate herbicide treatment to control this competitor. The study was carried out in a seven-year-old jack pine plantation north of Atikokan, Ontario.

  4. Estabelecimento e multiplicação in vitro de Prunus sp. em diferentes meios de cultivo Establishment and multiplication in vitro of Prunus sp. in different culture media

    Directory of Open Access Journals (Sweden)

    Alexandre Couto Rodrigues

    2003-04-01

    Full Text Available O trabalho foi realizado com o objetivo de avaliar diferentes meios de cultivo no estabelecimento e multiplicação in vitro de espécies do gênero Prunus. Os segmentos nodais de 1,0 cm foram mantidos sob luz fluorescente com radiação de 20 mE.m-2.s-1, fotoperíodo de 16 horas e temperatura de 25±2ºC. No estabelecimento, testaram os meios MS, MS ¾, SH e Villegas, e na multiplicação: SH e MS ¾. O meio MS ¾ foi testado em diferentes concentrações de ágar (4,5; 5,5; 6,5 g.L-1. Avaliaram-se as percentagens de estabelecimento dos explantes, contaminação, oxidação e segmentos não brotados. O meio Villegas apresentou menor oxidação durante o período de estabelecimento in vitro. Com o meio MS ¾, verificou-se maior percentagem de estabelecimento dos explantes. Na fase de multiplicação, avaliaram-se a percentagem de crescimento, a taxa de multiplicação e o número de brotações. O meio MS ¾, com 5,5 g.L-1 de ágar, apresentou os melhores resultados.The work was performed aiming to evaluate different media for the establishment and in vitro multiplication of some rootstocks species of Prunus. Microcutings of 1,0cm long were incubated under 20 mE.m-2.s-1 radiation provided by white fluorescent lamps, 16-hour photoperiod and temperature of 25±2ºC. The establishment media used were as follow: MS, ¾ MS, SH and Villegas, and the multiplication media used were: SH and ¾ MS. The ¾ MS medium was also tested with different agar concentrations (4.5; 5.5; 6.5 g.L-1. The percentage of establishment, contamination, oxidation and growing of the explants were evaluated. The Villegas medium provided low oxidation during the period of in vitro establishment. The ¾ MS medium provided higher percentage of establishment of explants. In the multiplication phase, the highest growth percentage, multiplication rate and bud number, were found in ¾ MS medium with 5.5 g.L-1 agar.

  5. Compared leaf anatomy and water relations of commercial and traditional Prunus dulcis (Mill.) cultivars under rain-fed conditions

    DEFF Research Database (Denmark)

    Oliveira, I.; Meyer, A.; Afonso, S.

    2018-01-01

    Leaf anatomy and water relations of seven almond (Prunus dulcis Mill.) cultivars, traditional (Bonita, Casanova, Parada, Pegarinhos and Verdeal) and commercial (Ferragnès and Glorieta), grown under rain-fed conditions, were studied. The performed measurements included thickness of leaf tissues...... cuticle thickness, while Pegarinhos adds a thicker epidermis and palisade parenchyma to increase protection to water loss. These data is one of the first comparative approaches to the leaf characterization of these cultivars, and should now be combined with physiological and biochemical studies...

  6. Genotyping-by-sequencing in an orphan plant species Physocarpus opulifolius helps identify the evolutionary origins of the genus Prunus.

    Science.gov (United States)

    Buti, Matteo; Sargent, Daniel J; Mhelembe, Khethani G; Delfino, Pietro; Tobutt, Kenneth R; Velasco, Riccardo

    2016-05-11

    The Rosaceae family encompasses numerous genera exhibiting morphological diversification in fruit types and plant habit as well as a wide variety of chromosome numbers. Comparative genomics between various Rosaceous genera has led to the hypothesis that the ancestral genome of the family contained nine chromosomes, however, the synteny studies performed in the Rosaceae to date encompass species with base chromosome numbers x = 7 (Fragaria), x = 8 (Prunus), and x = 17 (Malus), and no study has included species from one of the many Rosaceous genera containing a base chromosome number of x = 9. A genetic linkage map of the species Physocarpus opulifolius (x = 9) was populated with sequence characterised SNP markers using genotyping by sequencing. This allowed for the first time, the extent of the genome diversification of a Rosaceous genus with a base chromosome number of x = 9 to be performed. Orthologous loci distributed throughout the nine chromosomes of Physocarpus and the eight chromosomes of Prunus were identified which permitted a meaningful comparison of the genomes of these two genera to be made. The study revealed a high level of macro-synteny between the two genomes, and relatively few chromosomal rearrangements, as has been observed in studies of other Rosaceous genomes, lending further support for a relatively simple model of genomic evolution in Rosaceae.

  7. Development of novel techniques to extract phenolic compounds from Romanian cultivars of Prunus domestica L. and their biological properties.

    Science.gov (United States)

    Mocan, Andrei; Diuzheva, Alina; Carradori, Simone; Andruch, Vasil; Massafra, Chiara; Moldovan, Cadmiel; Sisea, Cristian; Petzer, Jacobus P; Petzer, Anél; Zara, Susi; Marconi, Guya Diletta; Zengin, Gokhan; Crișan, Gianina; Locatelli, Marcello

    2018-04-21

    In the present work, fourteen cultivars of Prunus domestica were analysed to investigate their phenolic pattern with the purpose of using the leaves as potential resources of bioactive compounds in the pharmaceutical and food industry. Microwave-assisted extraction (MAE), dispersive liquid-liquid microextraction and sugaring-out liquid-liquid extraction techniques were optimized in order to obtain an exhaustive multi-component panel of phenolic compounds. The best phenolic-enriched recovery was achieved using MAE in water:methanol (30:70), and this procedure was further applied for quantitative analysis of phenolic compounds in real samples. In order to prove the safeness of these extracts, the biological potential of the Prunus cultivars was tested by several in vitro antioxidant and enzyme inhibitory assays. Moreover, their cytotoxicity was evaluated on human gingival fibroblasts (HGFs), and in most of the cases the treatment with different concentrations of extracts didn't show cytotoxicity up to 500 μg/mL. Only 'Carpatin' and 'Minerva' cultivars, at 250 and 500 μg/mL, reduced partially cell viability of HGFs population. Noteworthy, Centenar cultivar was the most active for the α-glucosidase inhibition (6.77 mmolACAE/g extract), whereas Ialomița cultivar showed the best antityrosinase activity (23.07 mgKAE/g extract). Overall, leaves of P. domestica represent a rich alternative source of bioactive compounds. Copyright © 2018. Published by Elsevier Ltd.

  8. SSR allelic variation in almond (Prunus dulcis Mill.).

    Science.gov (United States)

    Xie, Hua; Sui, Yi; Chang, Feng-Qi; Xu, Yong; Ma, Rong-Cai

    2006-01-01

    Sixteen SSR markers including eight EST-SSR and eight genomic SSRs were used for genetic diversity analysis of 23 Chinese and 15 international almond cultivars. EST- and genomic SSR markers previously reported in species of Prunus, mainly peach, proved to be useful for almond genetic analysis. DNA sequences of 117 alleles of six of the 16 SSR loci were analysed to reveal sequence variation among the 38 almond accessions. For the four SSR loci with AG/CT repeats, no insertions or deletions were observed in the flanking regions of the 98 alleles sequenced. Allelic size variation of these loci resulted exclusively from differences in the structures of repeat motifs, which involved interruptions or occurrences of new motif repeats in addition to varying number of AG/CT repeats. Some alleles had a high number of uninterrupted repeat motifs, indicating that SSR mutational patterns differ among alleles at a given SSR locus within the almond species. Allelic homoplasy was observed in the SSR loci because of base substitutions, interruptions or compound repeat motifs. Substitutions in the repeat regions were found at two SSR loci, suggesting that point mutations operate on SSRs and hinder the further SSR expansion by introducing repeat interruptions to stabilize SSR loci. Furthermore, it was shown that some potential point mutations in the flanking regions are linked with new SSR repeat motif variation in almond and peach.

  9. Looking for the invisible universe - Black matter, black energy, black holes

    International Nuclear Information System (INIS)

    Elbaz, David

    2016-01-01

    As the discovery of the expansion of the universe and of black holes put the study of cosmology into question again because it now refers to invisible things such as black holes, black energy and black matter, the author proposes an other view on the universe within such a context. He first discusses these three enigmas of black matter, black energy and black holes. In a second part, he addresses, discusses and comments five illusions: the Uranian illusion (questions of the existence of an anti-world, of black matter temperature), the Mercurian illusion (quantum gravity, the string theory), the Martian illusion (a patchwork universe, the illusion of the infinite), the cosmic Maya (the John Wheeler's cup, the holographic universe), and the narcissistic illusion

  10. The Crisis in Black and Black.

    Science.gov (United States)

    Hutchinson, Earl Ofari

    These essays explore why the historic conflict between blacks and whites in the United States has become a crisis that divides many African Americans. The changing racial dynamic is not marked by conflicts. between the black middle class and the poor, black men and women, the black intellectual elite and rappers, black politicians and the urban…

  11. Vasorelaxant effect of Prunus yedoensis bark

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    Lee Kyungjin

    2013-02-01

    Full Text Available Abstract Background Prunus yedoensis Matsum. is used as traditional medicine—‘Yaeng-Pi’ or ‘Hua-Pi’—in Japan and Korea. However, no studies have examined the pharmacological activities of the P. yedoensis bark. Only the antioxidant and antiviral activities of P. yedoensis fruit and the anti-hyperglycaemic effect of P. yedoensis leaf have been investigated. While studying the antihypertensive effects of several medicinal plants, we found that a methanol extract of P. yedoensis bark (MEPY had distinct vasorelaxant effects on rat aortic rings. Methods The aortic rings were removed from Sprague–Dawley rats and suspended in organ chambers containing 10 ml Krebs-Henseleit solution. The aortic rings were placed between 2 tungsten stirrups and connected to an isometric force transducer. Changes in tension were recorded via isometric transducers connected to a data acquisition system. Results MEPY relaxed the contraction induced by phenylephrine (PE both in endothelium-intact and endothelium-denuded aortic rings concentration dependently. However, the vasorelaxant effects of MEPY on endothelium-denuded aortic rings were lower than endothelium-intact aortic rings. The vasorelaxant effects of MEPY on endothelium-intact aortic rings were reduced by pre-treatment with l-NAME, methylene blue, or ODQ. However, pre-treatment with indomethacin, atropine, glibenclamide, tetraethylammonium, or 4-aminopyridine had no affection. In addition, MEPY inhibited the contraction induced by extracellular Ca2+ in endothelium-denuded rat thoracic aorta rings pre-contracted by PE (1 μM or KCl (60 mM in Ca2+-free solution. Conclusions Our results suggest that MEPY exerts its vasorelaxant effects via the activation of NO formation by means of l-Arg and NO-cGMP pathways and via the blockage of extracellular Ca2+ channels.

  12. Sucrose and light effects on in vitro cultures of potato (Solanum tuberosum L.), chokecherry (Prunus virginiana L.) and Saskatoon berry (Amelanchier alnifolia Nutt.) during low temperature storage

    NARCIS (Netherlands)

    Pruski, K.; Kozai, T.; Lewis, T.; Astatkie, T.; Nowak, J.

    2000-01-01

    Cultures of potato (Solanum tuberosum) cv. Atlantic, chokecherry ( Prunus virginiana L.) cv. Garrington and saskatoon berry (Amelancher alnifolia Nutt.) cv. Northline grown in vitro for 3 weeks at 24/22 °C, 16-h photoperiod, 150 μmol m-2 s-1 photosynthetic photon flux density (PPFD) mixed

  13. Leaf cuticles as mediators of environmental influences: new developments in the use of isolated cuticles

    International Nuclear Information System (INIS)

    Scherbatskoy, T.

    1994-01-01

    Isolated leaf cuticles have been used in our research to characterize trans-cuticular ion diffusion rates under various environmental treatments and to measure cuticular attenuation of solar radiation. These studies have been conducted to understand better the role of the leaf cuticle as a protective barrier against potential environmental stressors including acid rain, ozone and ultraviolet radiation. These studies provide examples of a variety of current research uses for isolated leaf cuticles. Ion permeability coefficients and exchange rates in isolated cuticles of Acer, Prunus and Citrus species have been studied using several experimental approaches, including measurement of adsorption kinetics, electrical potential and conductance, and perfusion rates. Measured electrical (diffusion) potentials under KCl gradients across isolated cuticles are positive, indicating greater cation permeability. Electrical potentials (and permeability coefficients) vary with ionic strength and pH, and affect the driving force for ion diffusion through cuticles. Ion permeability in cuticles of Prunus serotina foliage was affected by experimental exposure to ozone and ultraviolet treatments. These studies indicate that cuticle permeability properties can be significantly altered by environmental factors. These and related studies on the ion exchange kinetics of Acer saccharum leaf cuticles suggest that foliar ''leaching'' is dominated by cuticle surface exchange mechanisms, with the magnitude of cuticular ion permeation being relatively small. In working with various hardwood species, we observed that the success rate for cuticle isolation varies with tree species and time of year. Scanning electron micrographs of inner cuticle surfaces indicate that the effects of enzymatic digestion could vary with exposure time, possibly affecting transport properties of isolated cuticles. Experimental work to test this in Citrus, however, showed no significant effect of isolation time on

  14. Effects of processing techniques on oxidative stability of Prunus pedunculatus seed oil

    Directory of Open Access Journals (Sweden)

    J. Yan

    2017-09-01

    Full Text Available This paper investigated the effects of Prunus pedunculatus (P. pedunculatus seed pre-treatment, including microwaving (M, roasting (R, steaming (S and roasting plus steaming (RS on crude oil quality in terms of yield, color change, fatty acid composition, and oxidative stability. The results showed an increase in monounsaturated fatty acid content and oxidative stability of the oils obtained from different processing treatments compared to the oil obtained from raw seeds (RW without processing. The oils, obtained from pretreated seeds, had higher conjugated diene (CD and 2-thiobarbituric acid (2-TBA values, compared to that obtained from RW when stored in a Schaal oven at 65 °C for 168 h. However, polyphenol and tocopherol contents decreased in all oil samples, processed or unprocessed. The effect of pre-treating the seeds was more prominent in the oil sample obtained through the RS technique, and showed higher oxidative stability than the other processed oils and the oil from RW.

  15. Future Applications of Apricot (Prunus Armeniaca Kaisa ß Galactosidase in Dairy Industry

    Directory of Open Access Journals (Sweden)

    Ansari Shakeel Ahmed

    2014-09-01

    Full Text Available The present study demonstrates the immobilization of β galactosidase from apricots (Prunus armeniaca kaisa on an inexpensive concanavalin A layered cellulose-alginate hybrid gel. Immobilized β galactosidase retained 78% of the initial activity after crosslinking by glutaraldehyde. It exhibited greater fraction of activity at both acidic and basic pH, and showed broad spectrum temperature optimum as compared to free enzyme. Moreover, immobilized enzyme exhibited higher thermal stability at 60°C and retained 80% of the original enzyme activity in presence of 3% galactose. The crosslinked immobilized enzyme showed improved hydrolysis of lactose from milk and whey in batch processes at 50°C as well as in continuous reactors operated at fl ow rate of 20 mL/h and 30 mL/h even after one month. Moreover, crosslinked adsorbed β galactosidase retained 76% activity even after its sixth repeated use, thereby promoting its use for lactose hydrolysis in various dairy products even for longer durations.

  16. Efeito do comprimento de estacas herbáceas de dois clones de umezeiro (Prunus mume Sieb & Zucc. no enraizamento adventício Effect of the length of herbaceous cuttings of two clones of japanese apricot (Prunus mume Sieb & Zucc. in adventicious rooting

    Directory of Open Access Journals (Sweden)

    NEWTON ALEX MAYER

    2002-08-01

    Full Text Available O umezeiro (Prunus mumeSieb & Zucc. é uma rosácea de folhas caducas, nativa da China, cujos frutos e flores são muito apreciados pelos povos orientais. No Brasil, alguns estudos foram realizados visando a sua utilização como porta-enxerto para pessegueiro e nectarineira, dadas as suas características de adaptação, rusticidade, redução do porte da planta e compatibilidade com algumas cultivares de Prunus persica. O presente estudo foi conduzido em câmara de nebulização sob ripado, pertencente ao Departamento de Produção Vegetal da FCAV/UNESP, Câmpus de Jaboticabal-SP. Objetivou-se verificar a influência de quatro comprimentos de estacas herbáceas no enraizamento de dois clones de umezeiro. O material vegetal, identificado como Clone 10 e Clone 15, foi oriundo do Programa de Melhoramento Genético do Instituto Agronômico de Campinas-SP. O experimento foi constituido de fatorial 2 x 4, em blocos casualizados, sendo o fator clone em 2 níveis (Clone 10 e Clone 15 e o fator comprimento de estaca em 4 níveis (12; 15; 18 e 25cm. Pelos resultados observados, verificou-se diferença entre os clones somente na porcentagem de estacas brotadas e número de raízes por estaca. O comprimento da estaca influenciou na porcentagem de enraizamento e na mortalidade das estacas, sendo que estacas maiores tenderam a apresentar maiores porcentagens de enraizamento e menores de mortalidade. As estacas com 12cm, embora apresentando menor número de raízes por estaca, são recomendadas por permitirem a obtenção de um maior número de estacas por planta-matriz. Houve efeito significativo da interação entre os fatores para número e comprimento de raízes.The japanese apricot (Prunus mume Sieb & Zucc. is a Rosaceae of falling leaves, native of China, whose fruits and flowers are quite appreciated by the oriental people. In Brazil, some studies were accomplished seeking its use as rootstock for peach and nectarine trees, due its adaptation

  17. Effects of cadmium on lipids of almond seedlings (Prunus dulcis).

    Science.gov (United States)

    Elloumi, Nada; Zouari, Mohamed; Chaari, Leila; Jomni, Chiraz; Marzouk, Brahim; Ben Abdallah, Ferjani

    2014-12-01

    Cadmium uptake and distribution, as well as its effects on lipid composition was investigated in almond seedlings (Prunus dulcis) grown in culture solution supplied with two concentrations of Cd (50 and 150 μM). The accumulation of Cd increased with external metal concentrations, and was considerably higher in roots than in leaves. Fourteen days after Cd treatment, the membrane lipids were extracted and separated on silica-gel thin layer chromatography (TLC). Fatty acid methyl esters were analyzed by FID-GC on a capillary column. Our results showed that Cd stress decreased the quantities of all lipids classes (phospholipids, galactolipids and neutral lipids). Galactolipid, phospholipid and neutral lipid concentrations decreased more in roots than in leaves by Cd-treatment. In almost all lipid classes the proportion of palmitic acid (16:0), linoleic (18: 2) and that of linolenic (18: 3) acid decreased, suggesting that heavy metal treatment induced an alteration in the fatty acid synthesis processes. In conclusion, our results show that the changes found in total fatty acids, in the quantities of all lipids classes, and in the in the profiles of individual polar lipids suggest that membrane structure and function might be altered by Cd stress.

  18. Some physico-chemical properties of Prunus armeniaca L. gum exudates.

    Science.gov (United States)

    Fathi, Morteza; Mohebbi, Mohebbat; Koocheki, Arash

    2016-01-01

    The objectives of this paper were to investigate some physicochemical properties of Prunus armeniaca L. gum exudates (PAGE). PAGE had, on average, 66.89% carbohydrate, 10.47% uronic acids, 6.9% moisture (w.b.), 2.91% protein, 4% ash and 1.59% fat. PAGE was composed of monosaccharides including l-arabinose, d-galactose, xylose, mannose and rhamnose in molar percentages of 41.52%, 23.72%, 17.82%, 14.40% and 2.54%, respectively. Elemental analysis showed that PAGE had high values of nutrients. FTIR analysis demonstrated the presence of carboxyl, hydroxyl and methyl groups and glycoside bonds. The weight average molecular weight, number average molecular weight and polydispersity index were found to be approximately 5.69 × 10(5)g/mol, 4.33 g/mol and 1.31, respectively. Rheological measurement of PAGE solutions as a function of concentration (8, 10 and 12% (w/w)) and temperature (10, 20, 30 and 40°C) demonstrated that the gum solutions had a non Newtonian shear thinning behaviour. Intrinsic viscosity for PAGE in deionized water was 3.438 dl/g based on Kramer equation. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. AVALIAÇÃO DA COMPATIBILIDADE DA ENXERTIA EM Prunus sp. EVALUATION OF THE GRAFT COMPATIBILITY IN Prunus sp.

    Directory of Open Access Journals (Sweden)

    ALEXANDRE COUTO RODRIGUES

    2001-08-01

    Full Text Available A atividade de peroxidase e concentração de fenóis foi determinada com o objetivo de se avaliar aspectos de compatibilidade entre porta-enxertos e enxertos. As amostras foram processadas e obtidas a partir da casca e lenho dos porta-enxertos de pessegueiros (GF 677, Okinawa, Capdeboscq e Aldrighi e de ameixeiras (Mirabolano e Marianna, enxertados ou não com as cultivares Diamante, Eldorado e Santa Rosa. Concluiu-se que a atividade de peroxidase e a concentração de fenóis foram relacionadas com união entre enxerto e porta-enxerto, particularmente, em Marianna e Mirabolano, onde a atividade de peroxidase e a concentração de fenóis foram mais elevados. A cultivar Santa Rosa foi compatível tanto com os porta-enxertos de ameixeiras quanto com os de pessegueiros.The work was accomplished aiming to quantify the peroxidase activity and total phenols, in order to verify the physiological and biochemical processes in grafting of Prunus sp. cultivars. The samples were processed and obtained in bark and wood of the peach rootstocks (GF 677, Okinawa, Capdeboscq and Aldrighi and plum rootstocks (Mirabolano and Marianna, after they had or not been grafted with the stock Diamante, Eldorado and Santa Rosa. It could be concluded that the peroxidase and the total phenols activity influenced the union between stock and rootstock; after grafting, the incompatibility degree is related with high peroxidase activity and total phenols in the rootstock Marianna and Mirabolano. The Santa Rosa plum graft is as compatible to plum rootstocks as to the peach ones.

  20. Tractor-mounted, GPS-based spot fumigation system manages Prunus replant disease

    Directory of Open Access Journals (Sweden)

    V. Udompetaikul

    2013-10-01

    Full Text Available Our research goal was to use recent advances in global positioning system (GPS and computer technology to apply just the right amount of fumigant where it is most needed (i.e., in a small target treatment zone in and around each tree replanting site to control Prunus replant disease (PRD. We developed and confirmed the function of (1 GPS-based software that can be used on cleared orchard land to flexibly plan and map all of an orchard's future tree sites and associated spot fumigation treatment zones and 2 a tractor-based GPS-controlled spot fumigation system to quickly and safely treat the targeted tree site treatment zones. In trials in two almond orchards and one peach orchard, our evaluations of the composite mapping and application system, which examined spatial accuracy of the spot treatments, delivery rate accuracy of the spot treatments, and tree growth responses to the spot treatments, all indicated that GPS spot fumigation has excellent potential to greatly reduce fumigant usage while adequately managing the PRD complex.

  1. Characterisation of stilbenes in California almonds (Prunus dulcis) by UHPLC-MS.

    Science.gov (United States)

    Xie, Liyang; Bolling, Bradley W

    2014-04-01

    Stilbene polyphenols are present in some fruits and nuts, but their abundance in many foods, such as almonds, is unknown. Therefore, we characterised stilbenes from Nonpareil, Butte and Carmel almond (Prunus dulcis) varieties from California. UHPLC-MS conditions were optimised to resolve cis- and trans-resveratrol, d4-resveratrol, dienestrol, hexestrol, oxyresveratrol, piceatannol, pterostilbene, and resveratrol-3-β-glucoside (polydatin). Stilbenes were isolated from ethanolic almond extracts by solid-phase extraction and identified with UHPLC-MS by comparison of retention times, mass spectra, in-source CID spectra, and enzymatic hydrolysis to authentic standards. Polydatin was identified in almond extracts, with 7.19-8.52 μg/100 g almond. Piceatannol+oxyresveratrol was tentatively identified in almond blanch water, at 0.19-2.55 μg/100 g almond. Polydatin was concentrated in almond skins, which contained 95.6-97.5% of the total almond content. Therefore, almonds contain the stilbene class of polyphenols in addition to the previously identified proanthocyanidin, hydrolysable tannin, flavonoid, and phenolic acid classes. Copyright © 2013 Elsevier Ltd. All rights reserved.

  2. Antioxidant Defenses in Plants with Attention to Prunus and Citrus spp.

    Directory of Open Access Journals (Sweden)

    Milvia Luisa Racchi

    2013-11-01

    Full Text Available This short review briefly introduces the formation of reactive oxygen species (ROS as by-products of oxidation/reduction (redox reactions, and the ways in which the antioxidant defense machinery is involved directly or indirectly in ROS scavenging. Major antioxidants, both enzymatic and non enzymatic, that protect higher plant cells from oxidative stress damage are described. Biochemical and molecular features of the antioxidant enzymes superoxide dismutase (SOD, catalase (CAT, and ascorbate peroxidase (APX are discussed because they play crucial roles in scavenging ROS in the different cell compartments and in response to stress conditions. Among the non enzymatic defenses, particular attention is paid to ascorbic acid, glutathione, flavonoids, carotenoids, and tocopherols. The operation of ROS scavenging systems during the seasonal cycle and specific developmental events, such as fruit ripening and senescence, are discussed in relation to the intense ROS formation during these processes that impact fruit quality. Particular attention is paid to Prunus and Citrus species because of the nutritional and antioxidant properties contained in these commonly consumed fruits.

  3. Diversità funzionale in cloni di ciliegio da legno (Prunus avium L. di provenienza Appennino toscano

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    Andrea Cutini

    2009-12-01

    Full Text Available Functionality in wild cherry (Prunus avium L. clones of Tuscany Appenines provenances. Results of a research regarding the functionality of already selected wild cherry (Prunus avium L. clones are reported. The main target was to select the genotypes with the best ecological efficiency and less sensible to environmental stress, in order to give concrete indications for arboriculture for wood productions. Starting from 2002, measurements were carried out in the experimental plot of Papiano (Stia, AR, where the following clones with provenance from the Tuscan Apennines were compared: Casina Alpe 1 (A, Casina Alpe 2 (D, Puzzòlo (C, Paradisino (E, Piantata Catenaia (F. Dendrometrical data were collected at the beginning and at the end of each season, in order to evaluate the growth and the individual current increment of the clones. To better characterize the canopies of each clone, measurements of photosynthetic active radiation (PAR and of the leaf area index (LAI where carried out with ceptometers and PCA LAI 2000. In order to evaluate differences between the clones regarding functionality and response to environmental stress, growth and productivity were related to the most important canopy characteristics. Ecological efficiency was calculated for the different clones using the net assimilation rate (NAR. The results show that the clone E has the most developed canopies and the best results in terms of growth. But at the same time it also presents densely branched round canopies and results more sensible to the effects of summer drought. These elements contribute to advise against the use of this clone in future genetic improvement programs and in high quality wood productions. On the contrary, the clones C and A have both good growth characteristics and a better general architecture and are therefore advised for high quality wood productions especially in the same geographic region.

  4. Green synthesis of nitrogen-doped graphitic carbon sheets with use of Prunus persica for supercapacitor applications

    Energy Technology Data Exchange (ETDEWEB)

    Atchudan, Raji, E-mail: atchudanr@yu.ac.kr [School of Chemical Engineering, Yeungnam University, Gyeongsan 38541 (Korea, Republic of); Edison, Thomas Nesakumar Jebakumar Immanuel [School of Chemical Engineering, Yeungnam University, Gyeongsan 38541 (Korea, Republic of); Perumal, Suguna [Department of Applied Chemistry, Kyungpook National University, Daegu 41566 (Korea, Republic of); Lee, Yong Rok, E-mail: yrlee@yu.ac.kr [School of Chemical Engineering, Yeungnam University, Gyeongsan 38541 (Korea, Republic of)

    2017-01-30

    Highlights: • N-GCSs was synthesized from the unripe Prunus persica by direct hydrothermal method. • The resulting N-GCSs-2 exhibit an excellent graphitization with 9.33% of nitrogen. • N-GCSs-2 provide high C{sub s} of 176 F g{sup −1} at current density of 0.1 A g{sup −1} in 1 M H{sub 2}SO{sub 4}. • N-GCSs-2 have high capacitance retention and 20% capacity growth after 2000 cycles. • First time, N-GCSs resulted from peach via green route for flexible supercapacitors. - Abstract: Nitrogen-doped graphitic carbon sheets (N-GCSs) were prepared from the extract of unripe Prunus persica fruit by a direct hydrothermal method. The synthesized N-GCSs were examined by high resolution transmission electron microscopy (HRTEM), nitrogen adsorption-desorption isotherms, X-ray diffraction (XRD), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FT-IR) spectroscopy. HRTEM showed that the synthesized carbon sheets were graphitic with lattice fringes and an inter-layer distance of 0.36 nm. Doping with the nitrogen moiety present over the synthesized GCSs was confirmed by XPS, FT-IR spectroscopy, and energy dispersive X-ray spectroscopy elemental mapping. The fruit extract associated with hydrothermal-carbonization method is economical and eco-friendly with a single step process. The resulting carbon sheets could be modified and are promising candidates for nano-electronic applications, including supercapacitors. The synthesized N-GCSs-2 provided a high specific capacitance of 176 F g{sup −1} at a current density of 0.1 A g{sup −1}. This electrode material has excellent cyclic stability, even after 2000 cycles of charge-discharge at a current density of 0.5 A g{sup −1}.

  5. Effects of processing techniques on oxidative stability of Prunus pedunculatus seed oil; Efectos de las técnicas de procesamiento sobre la estabilidad oxidativa del aceite de semilla de Prunus pedunculatus

    Energy Technology Data Exchange (ETDEWEB)

    Yan, J.; Guo, M.M.; Shen, Y. H.; Wang, Y.Y.; Luan, X.; Li, C.

    2017-07-01

    This paper investigated the effects of Prunus pedunculatus (P. pedunculatus) seed pre-treatment, including microwaving (M), roasting (R), steaming (S) and roasting plus steaming (RS) on crude oil quality in terms of yield, color change, fatty acid composition, and oxidative stability. The results showed an increase in monounsaturated fatty acid content and oxidative stability of the oils obtained from different processing treatments compared to the oil obtained from raw seeds (RW) without processing. The oils, obtained from pretreated seeds, had higher conjugated diene (CD) and 2-thiobarbituric acid (2-TBA) values, compared to that obtained from RW when stored in a Schaal oven at 65 °C for 168 h. However, polyphenol and tocopherol contents decreased in all oil samples, processed or unprocessed. The effect of pre-treating the seeds was more prominent in the oil sample obtained through the RS technique, and showed higher oxidative stability than the other processed oils and the oil from RW. [Spanish] Se investigó los efectos del pretratamiento de las semillas de Prunus pedunculatus, incluyendo microondas (M), tostado (R), cocción al vapor (S) y torrefacción más vapor (RS), sobre la calidad del aceite crudo, el rendimiento, cambio de color, composición en ácidos grasos y estabilidad oxidativa. Los resultados mostraron un aumento en el contenido de ácidos grasos monoinsaturados y en la estabilidad oxidativa de los aceites obtenidos con diferentes tratamientos de procesamiento en comparación con el aceite obtenido a partir de semillas crudas (RW) sin procesamiento. Los aceites obtenidos a partir de semillas pretratadas presentaron mayores valores de dienos conjugados (CD) y de ácido 2-tiobarbitúrico (2-TBA), comparado con el obtenido de RW cuando se almacenaron en horno a 65 °C durante 168 h. Sin embargo, el contenido de polifenoles y tocoferoles disminuyó en todas las muestras de aceites, procesadas o no procesadas. El efecto del pretratamiento de las

  6. Proteome analysis of the almond kernel (Prunus dulcis).

    Science.gov (United States)

    Li, Shugang; Geng, Fang; Wang, Ping; Lu, Jiankang; Ma, Meihu

    2016-08-01

    Almond (Prunus dulcis) is a popular tree nut worldwide and offers many benefits to human health. However, the importance of almond kernel proteins in the nutrition and function in human health requires further evaluation. The present study presents a systematic evaluation of the proteins in the almond kernel using proteomic analysis. The nutrient and amino acid content in almond kernels from Xinjiang is similar to that of American varieties; however, Xinjiang varieties have a higher protein content. Two-dimensional electrophoresis analysis demonstrated a wide distribution of molecular weights and isoelectric points of almond kernel proteins. A total of 434 proteins were identified by LC-MS/MS, and most were proteins that were experimentally confirmed for the first time. Gene ontology (GO) analysis of the 434 proteins indicated that proteins involved in primary biological processes including metabolic processes (67.5%), cellular processes (54.1%), and single-organism processes (43.4%), the main molecular function of almond kernel proteins are in catalytic activity (48.0%), binding (45.4%) and structural molecule activity (11.9%), and proteins are primarily distributed in cell (59.9%), organelle (44.9%), and membrane (22.8%). Almond kernel is a source of a wide variety of proteins. This study provides important information contributing to the screening and identification of almond proteins, the understanding of almond protein function, and the development of almond protein products. © 2015 Society of Chemical Industry. © 2015 Society of Chemical Industry.

  7. An efficient viral vector for functional genomic studies of Prunus fruit trees and its induced resistance to Plum pox virus via silencing of a host factor gene.

    Science.gov (United States)

    Cui, Hongguang; Wang, Aiming

    2017-03-01

    RNA silencing is a powerful technology for molecular characterization of gene functions in plants. A commonly used approach to the induction of RNA silencing is through genetic transformation. A potent alternative is to use a modified viral vector for virus-induced gene silencing (VIGS) to degrade RNA molecules sharing similar nucleotide sequence. Unfortunately, genomic studies in many allogamous woody perennials such as peach are severely hindered because they have a long juvenile period and are recalcitrant to genetic transformation. Here, we report the development of a viral vector derived from Prunus necrotic ringspot virus (PNRSV), a widespread fruit tree virus that is endemic in all Prunus fruit production countries and regions in the world. We show that the modified PNRSV vector, harbouring the sense-orientated target gene sequence of 100-200 bp in length in genomic RNA3, could efficiently trigger the silencing of a transgene or an endogenous gene in the model plant Nicotiana benthamiana. We further demonstrate that the PNRSV-based vector could be manipulated to silence endogenous genes in peach such as eukaryotic translation initiation factor 4E isoform (eIF(iso)4E), a host factor of many potyviruses including Plum pox virus (PPV). Moreover, the eIF(iso)4E-knocked down peach plants were resistant to PPV. This work opens a potential avenue for the control of virus diseases in perennial trees via viral vector-mediated silencing of host factors, and the PNRSV vector may serve as a powerful molecular tool for functional genomic studies of Prunus fruit trees. © 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

  8. A Cultural Resources Survey of Steele, New Franklin, and Main Ditches, and National Register of Historic Places Significance Testing of Sites 2PM574, 575, 577, and 23PM578, Pemiscot County Missouri

    Science.gov (United States)

    1990-07-15

    White (Quercus alba) 1 1 Pecan ( Carya illinoensis ) 1 1 Persimmon (Diospyros virginiana) T 2 2 Plum (Prunus sp.) T Red Haw (Crataegus sp.) T 1 11 Red... Carya sp.) 5 4 Shellbark (Car.ya laciniosa) T Hornbeam ( Ostrya virgin/iana) 2 Kentucky Coffee Tree(Gymnocladus dioica)T "Locust, ? T Black (Robinia

  9. Evaluation of multiple approaches to identify genome-wide polymorphisms in closely related genotypes of sweet cherry (Prunus avium L.

    Directory of Open Access Journals (Sweden)

    Seanna Hewitt

    Full Text Available Identification of genetic polymorphisms and subsequent development of molecular markers is important for marker assisted breeding of superior cultivars of economically important species. Sweet cherry (Prunus avium L. is an economically important non-climacteric tree fruit crop in the Rosaceae family and has undergone a genetic bottleneck due to breeding, resulting in limited genetic diversity in the germplasm that is utilized for breeding new cultivars. Therefore, it is critical to recognize the best platforms for identifying genome-wide polymorphisms that can help identify, and consequently preserve, the diversity in a genetically constrained species. For the identification of polymorphisms in five closely related genotypes of sweet cherry, a gel-based approach (TRAP, reduced representation sequencing (TRAPseq, a 6k cherry SNParray, and whole genome sequencing (WGS approaches were evaluated in the identification of genome-wide polymorphisms in sweet cherry cultivars. All platforms facilitated detection of polymorphisms among the genotypes with variable efficiency. In assessing multiple SNP detection platforms, this study has demonstrated that a combination of appropriate approaches is necessary for efficient polymorphism identification, especially between closely related cultivars of a species. The information generated in this study provides a valuable resource for future genetic and genomic studies in sweet cherry, and the insights gained from the evaluation of multiple approaches can be utilized for other closely related species with limited genetic diversity in the breeding germplasm. Keywords: Polymorphisms, Prunus avium, Next-generation sequencing, Target region amplification polymorphism (TRAP, Genetic diversity, SNParray, Reduced representation sequencing, Whole genome sequencing (WGS

  10. Unusual behavior of growing pollen tubes in the ovary of plum culture (Prunus domestica L.

    Directory of Open Access Journals (Sweden)

    Đorđević Milena

    2010-01-01

    Full Text Available Unusual behavior of growing pollen tubes in different combinations of pollination was observed in the ovary of the plum (Prunus domestica L. cv 'Čačanska Lepotica'. It primarily refers to several issues, i.e. the curling up of pollen tubes within the micropyle, the growth of two pollen tubes into the nucellus of an ovule, the occurrence of a bundle above the nucellar cap and fluorescence of the part of the embryo sac containing the egg apparatus. Upon the growth of pollen tubes into the nucellus of the ovule, subsequently penetrating pollen tubes form a bundle either above the micropyle entrance or above the nucellus. Branching and bending of pollen tubes by 180o upon their growth into the micropyle was also observed.

  11. Ectopic expression of class 1 KNOX genes induce and adventitious shoot regeneration and alter growth and development of tobacco (Nicotiana tabacum L) and European plum (Prunus domestica L)

    Science.gov (United States)

    Transgenic plants of tobacco (Nicotiana tabacum L) and plum (Prunus domestica L) were produced by transforming with apple class 1 KNOX genes (MdKN1 and MdKN2) or corn KN1 gene. Transgenic tobacco plants were regenerated in vitro from transformed leaf discs cultured in a tissue medium lacking cytoki...

  12. Adsorption Properties of Low-Cost Biomaterial Derived from Prunus amygdalus L. for Dye Removal from Water

    Science.gov (United States)

    Deniz, Fatih

    2013-01-01

    The capability of Prunus amygdalus L. (almond) shell for dye removal from aqueous solutions was investigated and methyl orange was used as a model compound. The effects of operational parameters including pH, ionic strength, adsorbent concentration and mesh size, dye concentration, contact time, and temperature on the removal of dye were evaluated. The adsorption kinetics conformed to the pseudo-second-order kinetic model. The equilibrium data pointed out excellent fit to the Langmuir isotherm model with maximum monolayer adsorption capacity of 41.34 mg g−1 at 293 K. Thermodynamic analysis proved a spontaneous, favorable, and exothermic process. It can be concluded that almond shell might be a potential low-cost adsorbent for methyl orange removal from aqueous media. PMID:23935442

  13. Purification, identification and preliminary crystallographic studies of Pru du amandin, an allergenic protein from Prunus dulcis.

    Science.gov (United States)

    Gaur, Vineet; Sethi, Dhruv K; Salunke, Dinakar M

    2008-01-01

    Food allergies appear to be one of the foremost causes of hypersensitivity reactions. Nut allergies account for most food allergies and are often permanent. The 360 kDa hexameric protein Pru du amandin, a known allergen, was purified from almonds (Prunus dulcis) by ammonium sulfate fractionation and ion-exchange chromatography. The protein was identified by a BLAST homology search against the nonredundant sequence database. Pru du amandin belongs to the 11S legumin family of seed storage proteins characterized by the presence of a cupin motif. Crystals were obtained by the hanging-drop vapour-diffusion method. The crystals belong to space group P4(1) (or P4(3)), with unit-cell parameters a = b = 150.7, c = 164.9 A.

  14. Phylogeny of isolates of Prunus necrotic ringspot virus from the Ilarvirus Ringtest and identification of group-specific features.

    Science.gov (United States)

    Hammond, R W

    2003-06-01

    Isolates of Prunus necrotic ringspot virus (PNRSV) were examined to establish the level of naturally occurring sequence variation in the coat protein (CP) gene and to identify group-specific genome features that may prove valuable for the generation of diagnostic reagents. Phylogenetic analysis of a 452 bp sequence of 68 virus isolates, 20 obtained from the European Union Ilarvirus Ringtest held in October 1998, confirmed the clustering of the isolates into three distinct groups. Although no correlation was found between the sequence and host or geographic origin, there was a general trend for severe isolates to cluster into one group. Group-specific features have been identified for discrimination between virus strains.

  15. Antiproliferative terpenoids from almond hulls (Prunus dulcis): identification and structure-activity relationships.

    Science.gov (United States)

    Amico, Vincenzo; Barresi, Vincenza; Condorelli, Daniele; Spatafora, Carmela; Tringali, Corrado

    2006-02-08

    Bioassay-guided fractionation of the EtOAc crude extract from Sicilian almond hulls, a waste material from Prunus dulcis crop, allowed identification of 10 constituents, isolated as pure compounds (1-5, 7, and 10) or unseparable mixtures (5 + 6 and 8 + 9). All compounds were subjected to spectroscopic analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide bioassay on MCF-7 human breast cancer cells. In addition to the main components oleanolic (1), ursolic (2), and betulinic (3) acids, the 2-hydroxy analogues alphitolic (4), corosolic (5), and maslinic (6) acids, as well as the related aldehydes, namely, betulinic (7), oleanolic (8), and ursolic (9), were identified. From a more polar fraction, the beta-sitosterol 3-O-glucoside (10) was also identified. A sample of commercially available betulin (11) was also included in bioassays as further support to a structure-activity relationship study. Betulinic acid showed antiproliferative activity toward MCF-7 cells (GI50 = 0.27 microM), higher than the anticancer drug 5-fluorouracil.

  16. [Procedure of seed quality testing and seed grading standard of Prunus humilis].

    Science.gov (United States)

    Wen, Hao; Ren, Guang-Xi; Gao, Ya; Luo, Jun; Liu, Chun-Sheng; Li, Wei-Dong

    2014-11-01

    So far there exists no corresponding quality test procedures and grading standards for the seed of Prunus humilis, which is one of the important source of base of semen pruni. Therefor we set up test procedures that are adapt to characteristics of the P. humilis seed through the study of the test of sampling, seed purity, thousand-grain weight, seed moisture, seed viability and germination percentage. 50 cases of seed specimens of P. humilis tested. The related data were analyzed by cluster analysis. Through this research, the seed quality test procedure was developed, and the seed quality grading standard was formulated. The seed quality of each grade should meet the following requirements: for first grade seeds, germination percentage ≥ 68%, thousand-grain weight 383 g, purity ≥ 93%, seed moisture ≤ 5%; for second grade seeds, germination percentage ≥ 26%, thousand-grain weight ≥ 266 g, purity ≥ 73%, seed moisture ≤9%; for third grade seeds, germination percentage ≥ 10%, purity ≥ 50%, thousand-grain weight ≥ 08 g, seed moisture ≤ 13%.

  17. Chlorophyll Catabolites in Senescent Leaves of the Plum Tree (Prunus domestica).

    Science.gov (United States)

    Erhart, Theresia; Mittelberger, Cecilia; Vergeiner, Clemens; Scherzer, Gerhard; Holzner, Barbara; Robatscher, Peter; Oberhuber, Michael; Kräutler, Bernhard

    2016-11-01

    In cold extracts of senescent leaves of the plum tree (Prunus domestica ssp. domestica), six colorless non-fluorescent chlorophyll catabolites (NCCs) were characterized, named Pd-NCCs. In addition, several minor NCC fractions were tentatively classified. The structure of the most polar one of the NCCs, named Pd-NCC-32, featured an unprecedented twofold glycosidation pattern. Three of the NCCs are also functionalized at their 3 2 -position by a glucopyranosyl group. In addition, two of these glycosidated NCCs carry a dihydroxyethyl group at their 18-position. In the polar Pd-NCC-32, the latter group is further glycosidated at the terminal 18 2 -position. Four other major Pd-NCCs and one minor Pd-NCC were identified with five NCCs from higher plants known to belong to the 'epi'-series. In addition, tentative structures were derived for two minor fractions, classified as yellow chlorophyll catabolites, which represented (formal) oxidation products of two of the observed Pd-NCCs. The chlorophyll catabolites in leaves of plum feature the same basic structural pattern as those found in leaves of apple and pear trees. © 2016 The Authors. Chemistry & Biodiversity Published by Wiley-VHCA AG, Zurich, Switzerland.

  18. Black Silicon Solar Cells with Black Ribbons

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Tang, Peter Torben; Mizushima, Io

    2016-01-01

    We present the combination of mask-less reactive ion etch (RIE) texturing and blackened interconnecting ribbons as a method for obtaining all-black solar panels, while using conventional, front-contacted solar cells. Black silicon made by mask-less reactive ion etching has total, average...... in the range 15.7-16.3%. The KOH-textured reference cell had an efficiency of 17.9%. The combination of black Si and black interconnecting ribbons may result in aesthetic, all-black panels based on conventional, front-contacted silicon solar cells....... reflectance below 0.5% across a 156x156 mm2 silicon (Si) wafer. Black interconnecting ribbons were realized by oxidizing copper resulting in reflectance below 3% in the visible wavelength range. Screen-printed Si solar cells were realized on 156x156 mm2 black Si substrates with resulting efficiencies...

  19. Seasonal variation of Prunus necrotic ringspot virus concentration in almond, peach, and plum cultivars

    Directory of Open Access Journals (Sweden)

    N. Salem

    2003-08-01

    Full Text Available Levels of Prunus necrotic ringspot virus (PNRSV infection in almond, peach, and plum cultivars over the course of an entire year were determined by testing different plant parts of naturally infected trees, using the double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA. The data showed that spring was the best time of year for PNRSV detection in flowers, active growing buds, and young leaves. PNRSV detection was less reliable during the summer months. Young leaves of all cultivars were the most reliable source for distinguishing between healthy and infected plants, while flowers and buds yielded high values in some cultivars but not in others. Seasonal fluctuations in virus concentration did not follow the same pattern in all cultivars. It is therefore impossible to distinguish between infected and healthy trees on the basis of one single sampling time for all cultivars.

  20. A Quantitative Index of Forest Structural Sustainability

    Directory of Open Access Journals (Sweden)

    Jonathan A. Cale

    2014-07-01

    Full Text Available Forest health is a complex concept including many ecosystem functions, interactions and values. We develop a quantitative system applicable to many forest types to assess tree mortality with respect to stable forest structure and composition. We quantify impacts of observed tree mortality on structure by comparison to baseline mortality, and then develop a system that distinguishes between structurally stable and unstable forests. An empirical multivariate index of structural sustainability and a threshold value (70.6 derived from 22 nontropical tree species’ datasets differentiated structurally sustainable from unsustainable diameter distributions. Twelve of 22 species populations were sustainable with a mean score of 33.2 (median = 27.6. Ten species populations were unsustainable with a mean score of 142.6 (median = 130.1. Among them, Fagus grandifolia, Pinus lambertiana, P. ponderosa, and Nothofagus solandri were attributable to known disturbances; whereas the unsustainability of Abies balsamea, Acer rubrum, Calocedrus decurrens, Picea engelmannii, P. rubens, and Prunus serotina populations were not. This approach provides the ecological framework for rational management decisions using routine inventory data to objectively: determine scope and direction of change in structure and composition, assess excessive or insufficient mortality, compare disturbance impacts in time and space, and prioritize management needs and allocation of scarce resources.

  1. GENETIC DIVERSITY OF SOME IRANIAN SWEET CHERRY (PRUNUS AVIUM) CULTIVARS USING MICROSATELLITE MARKERS AND MORPHOLOGICAL TRAITS.

    Science.gov (United States)

    Farsad, A; Esna-Ashari, M

    2016-01-01

    The aim of this study was to characterize 23 important Iranian sweet cherry (Prunus avium) cultivars collected from different provinces of Iran and 1 foreign cultivar, which was used as control, considered for breeding programs by using 21 microsatellite markers and 27 morphological traits. In sweet cherry (Prunus avium) accessions, leaf, fruit, and stone morphological characters were evaluated during two consecutive years. The study revealed a high variability in the set of evaluated sweet cherry accessions. The majority of important correlations were determined among variables representing fruit and leaf size and variables related to color. Cluster analysis distinguished sweet cherry accessions into two distinct groups. Principal component analysis (PCA) of qualitative and quantitative morphological parameters explained over 86.59% of total variability in the first seven axes. In PCA, leaf traits such as leaf length and width, and fruit traits such as length, width, and weight, and fruit flesh and juice color were predominant in the first two components, indicating that they were useful for the assessment of sweet cherry germplasm characterization. Out of 21 SSR markers, 16 were polymorphic, producing 177 alleles that varied from 4 to 16 alleles (9.35 on average) with a mean heterozygosity value of 0.82 that produced successful amplifications and revealed DNA polymorphisms. Allele size varied from 95 to 290 bp. Cluster analyses showed that the studied sweet cherry genotypes were classified intofive main groups based mainly on their species characteristics and SSR data. In general, our results did not show a clear structuring of genetic variability within the Iranian diffusion area of sweet cherry, so it was not possible to draw any indications on regions of provenance delimitation. The results of this study contribute to a better understanding of sweet cherry genetic variations in Iran, thus making for more efficient programs aimed at preserving biodiversity and

  2. Black holes

    International Nuclear Information System (INIS)

    Feast, M.W.

    1981-01-01

    This article deals with two questions, namely whether it is possible for black holes to exist, and if the answer is yes, whether we have found any yet. In deciding whether black holes can exist or not the central role in the shaping of our universe played by the forse of gravity is discussed, and in deciding whether we are likely to find black holes in the universe the author looks at the way stars evolve, as well as white dwarfs and neutron stars. He also discusses the problem how to detect a black hole, possible black holes, a southern black hole, massive black holes, as well as why black holes are studied

  3. Evolutionary patterns at the RNase based gametophytic self - incompatibility system in two divergent Rosaceae groups (Maloideae and Prunus).

    Science.gov (United States)

    Vieira, Jorge; Ferreira, Pedro G; Aguiar, Bruno; Fonseca, Nuno A; Vieira, Cristina P

    2010-06-28

    Within Rosaceae, the RNase based gametophytic self-incompatibility (GSI) system has been studied at the molecular level in Maloideae and Prunus species that have been diverging for, at least, 32 million years. In order to understand RNase based GSI evolution within this family, comparative studies must be performed, using similar methodologies. It is here shown that many features are shared between the two species groups such as levels of recombination at the S-RNase (the S-pistil component) gene, and the rate at which new specificities arise. Nevertheless, important differences are found regarding the number of ancestral lineages and the degree of specificity sharing between closely related species. In Maloideae, about 17% of the amino acid positions at the S-RNase protein are found to be positively selected, and they occupy about 30% of the exposed protein surface. Positively selected amino acid sites are shown to be located on either side of the active site cleft, an observation that is compatible with current models of specificity determination. At positively selected amino acid sites, non-conservative changes are almost as frequent as conservative changes. There is no evidence that at these sites the most drastic amino acid changes may be more strongly selected. Many similarities are found between the GSI system of Prunus and Maloideae that are compatible with the single origin hypothesis for RNase based GSI. The presence of common features such as the location of positively selected amino acid sites and lysine residues that may be important for ubiquitylation, raise a number of issues that, in principle, can be experimentally addressed in Maloideae. Nevertheless, there are also many important differences between the two Rosaceae GSI systems. How such features changed during evolution remains a puzzling issue.

  4. Evolutionary patterns at the RNase based gametophytic self - incompatibility system in two divergent Rosaceae groups (Maloideae and Prunus

    Directory of Open Access Journals (Sweden)

    Fonseca Nuno A

    2010-06-01

    Full Text Available Abstract Background Within Rosaceae, the RNase based gametophytic self-incompatibility (GSI system has been studied at the molecular level in Maloideae and Prunus species that have been diverging for, at least, 32 million years. In order to understand RNase based GSI evolution within this family, comparative studies must be performed, using similar methodologies. Result It is here shown that many features are shared between the two species groups such as levels of recombination at the S-RNase (the S-pistil component gene, and the rate at which new specificities arise. Nevertheless, important differences are found regarding the number of ancestral lineages and the degree of specificity sharing between closely related species. In Maloideae, about 17% of the amino acid positions at the S-RNase protein are found to be positively selected, and they occupy about 30% of the exposed protein surface. Positively selected amino acid sites are shown to be located on either side of the active site cleft, an observation that is compatible with current models of specificity determination. At positively selected amino acid sites, non-conservative changes are almost as frequent as conservative changes. There is no evidence that at these sites the most drastic amino acid changes may be more strongly selected. Conclusions Many similarities are found between the GSI system of Prunus and Maloideae that are compatible with the single origin hypothesis for RNase based GSI. The presence of common features such as the location of positively selected amino acid sites and lysine residues that may be important for ubiquitylation, raise a number of issues that, in principle, can be experimentally addressed in Maloideae. Nevertheless, there are also many important differences between the two Rosaceae GSI systems. How such features changed during evolution remains a puzzling issue.

  5. Sugar maple growth in relation to nutrition and stress in the northeastern United States.

    Science.gov (United States)

    Long, Robert P; Horsley, Stephen B; Hallett, Richard A; Bailey, Scott W

    2009-09-01

    Sugar maple, Acer saccharum, decline disease is incited by multiple disturbance factors when imbalanced calcium (Ca), magnesium (Mg), and manganese (Mn) act as predisposing stressors. Our objective in this study was to determine whether factors affecting sugar maple health also affect growth as estimated by basal area increment (BAI). We used 76 northern hardwood stands in northern Pennsylvania, New York, Vermont, and New Hampshire, USA, and found that sugar maple growth was positively related to foliar concentrations of Ca and Mg and stand level estimates of sugar maple crown health during a high stress period from 1987 to 1996. Foliar nutrient threshold values for Ca, Mg, and Mn were used to analyze long-term BAI trends from 1937 to 1996. Significant (P maples sampled in the 1990s had decreased growth in the 1970s, 10-20 years in advance of the 1980s and 1990s decline episode in Pennsylvania. Even apparently healthy stands that had no defoliation, but had below-threshold amounts of Ca or Mg and above-threshold Mn (from foliage samples taken in the mid 1990s), had decreasing growth by the 1970s. Co-occurring black cherry, Prunus serotina, in a subset of the Pennsylvania and New York stands, showed opposite growth responses with greater growth in stands with below-threshold Ca and Mg compared with above-threshold stands. Sugar maple growing on sites with the highest concentrations of foliar Ca and Mg show a general increase in growth from 1937 to 1996 while other stands with lower Ca and Mg concentrations show a stable or decreasing growth trend. We conclude that acid deposition induced changes in soil nutrient status that crossed a threshold necessary to sustain sugar maple growth during the 1970s on some sites. While nutrition of these elements has not been considered in forest management decisions, our research shows species specific responses to Ca and Mg that may reduce health and growth of sugar maple or change species composition, if not addressed.

  6. Identification of volatile compounds in thinning discards from plum trees (Prunus salicina Lindl. cultivar Harry Pickstone

    Directory of Open Access Journals (Sweden)

    Rossana Podestá

    2011-09-01

    Full Text Available Plum (Prunus salicina Lindl. cv. Harry Pickstone, a China indigenous fruit, is widely produced and consumed in countries such as Japan and Brazil. The practice of thinning is common in horticulture and the fruits removed are discarded as waste. Like the great majority of vegetables, these thinning discards also contain essential oils which have not been investigated until the present time. The extraction of the plum thinning discards volatile oil, through the hydrodistillation method, produced a yield of 0.06% (m/m and a total of 21 components were identified, with 11 of them being responsible for 72,9% of the total oil composition. The major compounds determined through GC and GC-MS were Z-α-bisabolene (13.7%, n-hexadecanoic acid (12.7%, phytol (12.7%, and β-caryophyllene (10.4%.

  7. Black silicon with black bus-bar strings

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Tang, Peter Torben; Mizushima, Io

    2016-01-01

    We present the combination of black silicon texturing and blackened bus-bar strings as a potential method for obtaining all-black solar panels, while using conventional, front-contacted solar cells. Black silicon was realized by mask-less reactive ion etching resulting in total, average reflectance...... below 0.5% across a 156x156 mm2 silicon wafer. Black bus-bars were realized by oxidized copper resulting in reflectance below 3% in the entire visible wavelength range. The combination of these two technologies may result in aesthetic, all-black panels based on conventional, front-contacted solar cells...

  8. Mutational analysis of the RNA-binding domain of the Prunus necrotic ringspot virus (PNRSV) movement protein reveals its requirement for cell-to-cell movement

    OpenAIRE

    Herranz, M. Carmen; Sánchez Navarro, Jesús A.; Saurí Peris, Ana; Mingarro Muñoz, Ismael; Pallás Benet, Vicente

    2005-01-01

    The movement protein (MP) of Prunus necrotic ringspot virus (PNRSV) is required for cell-to-cell movement. MP subcellular localization studies using a GFP fusion protein revealed highly punctate structures between neighboring cells, believed to represent plasmodesmata. Deletion of the RNA-binding domain (RBD) of PNRSV MP abolishes the cell-to-cell movement. A mutational analysis on this RBD was performed in order to identify in vivo the features that govern viral transport. Loss of positive c...

  9. Sobrevivencia del duraznillo (Prunus annularis en plantación forestal y en sistemas agroforestales

    Directory of Open Access Journals (Sweden)

    Javier Monge

    2005-01-01

    Full Text Available Se estudió la sobrevivencia inicial (30 meses del duraznillo (Prunus annularis en plantación forestal y en sistema agroforestal en la zona de vida Bosque muy Húmedo Montano Bajo, en Costa Rica. Se evaluó 5 tratamientos, en 2 lotes con 2 repeticiones en cada lote, en parcelas de 25 árboles (722 ha-1. Los sistemas de producción evaluados fueron: plantación forestal, con manejo de eliminación de malezas cada 4 meses (PF-4 y cada 2 meses (PF-2, y sistemas agroforestales duraznillo-naranjilla (Solanum quitoense, duraznillo-menta (Satureja viminea y duraznillo-maíz (Zea mays. La sobrevivencia a los 30 meses osciló entre 56 y 81% siendo menor en plantación forestal (PF-4. La sobrevivencia mostrada por el duraznillo se consideró intermedia con respecto a otras especies establecidas en sitios con la misma zona de vida.

  10. Microencapsulation of plum (Prunus salicina Lindl. phenolics by spray drying technology and storage stability

    Directory of Open Access Journals (Sweden)

    Yibin LI

    2017-10-01

    Full Text Available Abstract To improve the stability of the phenolic extracts from plum fruit (Prunus salicina Lindl., the microencapsulation conditions of spray drying were optimized by the response surface method. The Box-Behnken experimental results indicated the optimal conditions involved an inlet air temperature of 142.8 °C, a core material content of 23.7% and a feed solids content of 11.7%. The maximum microencapsulating efficiency was 87.7% at optimal conditions. Further, the physicochemical properties of the microcapsule powders were improved overall due to the addition of the coating agents. There were no statistically significant differences in phenolic content of the obtained microcapsules for the first 40 days of storage at 25 °C in dark condition (p > 0.05, and the retention rate of total phenol remained above 85% after 60 days. Microcapsules can be potentially developed as a source of natural pigment or functional food based on the advantages of rich phenolic compounds and red color.

  11. Black rings

    International Nuclear Information System (INIS)

    Emparan, Roberto; Reall, Harvey S

    2006-01-01

    A black ring is a five-dimensional black hole with an event horizon of topology S 1 x S 2 . We provide an introduction to the description of black rings in general relativity and string theory. Novel aspects of the presentation include a new approach to constructing black ring coordinates and a critical review of black ring microscopics. (topical review)

  12. "Black Like Me": Reframing Blackness for Decolonial Politics

    Science.gov (United States)

    Dei, George J. Sefa

    2018-01-01

    From a particular vantage point, as an African-born scholar with a politics to affirm my Black subjectivity and Indigeneity in a diasporic context, my article engages a (re)theorization of Blackness for decolonial politics. Building on existing works of how Black scholars, themselves, have theorized Blackness, and recognizing the fluid,…

  13. VPLIV UPORABE RASTNIH HORMONOV V RAZLIČNIH RAZVOJNIH FAZAH PLODOV NA KOLIČINO IN KAKOVOST PRIDELKA ČEŠNJE (Prunus avium L.)

    OpenAIRE

    Pelc, David

    2015-01-01

    V letu 2008 smo v Sadjarskem centru Maribor – Gačnik spremljali vpliv uporabe rastnih hormonov v različnih razvojnih fazah plodov na količino in kakovost pridelka češnje (Prunus avium L.). Namen poskusa je bil ugotoviti, ali rastni hormoni (avksini, citokinini) lahko pri češnji vplivajo na kakovost, velikost in na količino pridelka. Vključenih je bilo 6 obravnavanj, od katerih so 3 obravnavanja predstavljala tretiranje z avksini (Amid-thin, Maxim), 1 obravnavanje s citokinini (CPPU), 1 obravn...

  14. Adaptive covariation between the coat and movement proteins of prunus necrotic ringspot virus.

    Science.gov (United States)

    Codoñer, Francisco M; Fares, Mario A; Elena, Santiago F

    2006-06-01

    The relative functional and/or structural importance of different amino acid sites in a protein can be assessed by evaluating the selective constraints to which they have been subjected during the course of evolution. Here we explore such constraints at the linear and three-dimensional levels for the movement protein (MP) and coat protein (CP) encoded by RNA 3 of prunus necrotic ringspot ilarvirus (PNRSV). By a maximum-parsimony approach, the nucleotide sequences from 46 isolates of PNRSV varying in symptomatology, host tree, and geographic origin have been analyzed and sites under different selective pressures have been identified in both proteins. We have also performed covariation analyses to explore whether changes in certain amino acid sites condition subsequent variation in other sites of the same protein or the other protein. These covariation analyses shed light on which particular amino acids should be involved in the physical and functional interaction between MP and CP. Finally, we discuss these findings in the light of what is already known about the implication of certain sites and domains in structure and protein-protein and RNA-protein interactions.

  15. Molecular mechanisms regulating flowering time in sweet cherry (Prunus avium L.)

    DEFF Research Database (Denmark)

    Ionescu, Irina Alexandra

    The timing of flowering is a well-researched but at the same time incredibly complex process in angiosperms. Although we are in possession of detailed knowledge on the genetic level of flowering time regulation in the model plant Arabidopsis thaliana, it is often difficult to transfer this knowle......The timing of flowering is a well-researched but at the same time incredibly complex process in angiosperms. Although we are in possession of detailed knowledge on the genetic level of flowering time regulation in the model plant Arabidopsis thaliana, it is often difficult to transfer...... as a result of hydrogen cyanamide treatment: the jasmonate pathway, the hydrogen cyanide pathway and the cytokinin pathway. We further analyzed the levels of cyanogenic glucosides and their derivatives during endodormancy and its release in sweet cherry and almond (Prunus dulcis (Mill.) D. A. Webb). Prunasin...... and its amide coincided with flowering time in both species. Taken together, these results contribute to elucidating parts of the complex network regulating flowering time in perennial plants....

  16. Wound Healing Effects of Prunus yedoensis Matsumura Bark in Scalded Rats

    Directory of Open Access Journals (Sweden)

    Jin-Ho Lee

    2017-01-01

    Full Text Available Pruni Cortex has been used to treat asthma, measles, cough, urticaria, pruritus, and dermatitis in traditional Korean medicine. The objective of this study was to investigate the effects of Prunus yedoensis Matsumura bark methanol extract (PYE on scald-induced dorsal skin wounds in rats. Scalds were produced in Sprague-Dawley rats with 100°C water and treated with 5% and 20% PYE (using Vaseline as a base, silver sulfadiazine (SSD, and Vaseline once a day for 21 days, beginning 24 hours after scald by treatment group allocation. The PYE-treated groups showed accelerated healing from 12 days after scald, demonstrated by rapid eschar exfoliation compared to the control and SSD groups. PYE-treated groups showed higher wound contraction rates and better tissue regeneration in comparison with the control group. Serum analysis showed that transforming growth factor beta 1 and vascular endothelial growth factor levels remained high or gradually increased up to day 14 in both PYE groups and then showed a sharp decline by day 21, implying successful completion of the inflammatory phase and initiation of tissue regeneration. These findings suggested that PYE is effective in promoting scald wound healing in the inflammation and tissue proliferation stages.

  17. De novo transcriptome assembly and comparative analysis of differentially expressed genes in Prunus dulcis Mill. in response to freezing stress.

    Directory of Open Access Journals (Sweden)

    Sadegh Mousavi

    Full Text Available Almond (Prunus dulcis Mill., one of the most important nut crops, requires chilling during winter to develop fruiting buds. However, early spring chilling and late spring frost may damage the reproductive tissues leading to reduction in the rate of productivity. Despite the importance of transcriptional changes and regulation, little is known about the almond's transcriptome under the cold stress conditions. In the current research, we used RNA-seq technique to study the response of the reproductive tissues of almond (anther and ovary to frost stress. RNA sequencing resulted in more than 20 million reads from anther and ovary tissues of almond, individually. About 40,000 contigs were assembled and annotated de novo in each tissue. Profile of gene expression in ovary showed significant alterations in 5,112 genes, whereas in anther 6,926 genes were affected by freezing stress. Around two thousands of these genes were common altered genes in both ovary and anther libraries. Gene ontology indicated the involvement of differentially expressed (DE genes, responding to freezing stress, in metabolic and cellular processes. qRT-PCR analysis verified the expression pattern of eight genes randomly selected from the DE genes. In conclusion, the almond gene index assembled in this study and the reported DE genes can provide great insights on responses of almond and other Prunus species to abiotic stresses. The obtained results from current research would add to the limited available information on almond and Rosaceae. Besides, the findings would be very useful for comparative studies as the number of DE genes reported here is much higher than that of any previous reports in this plant.

  18. De novo transcriptome assembly and comparative analysis of differentially expressed genes in Prunus dulcis Mill. in response to freezing stress.

    Science.gov (United States)

    Mousavi, Sadegh; Alisoltani, Arghavan; Shiran, Behrouz; Fallahi, Hossein; Ebrahimie, Esameil; Imani, Ali; Houshmand, Saadollah

    2014-01-01

    Almond (Prunus dulcis Mill.), one of the most important nut crops, requires chilling during winter to develop fruiting buds. However, early spring chilling and late spring frost may damage the reproductive tissues leading to reduction in the rate of productivity. Despite the importance of transcriptional changes and regulation, little is known about the almond's transcriptome under the cold stress conditions. In the current research, we used RNA-seq technique to study the response of the reproductive tissues of almond (anther and ovary) to frost stress. RNA sequencing resulted in more than 20 million reads from anther and ovary tissues of almond, individually. About 40,000 contigs were assembled and annotated de novo in each tissue. Profile of gene expression in ovary showed significant alterations in 5,112 genes, whereas in anther 6,926 genes were affected by freezing stress. Around two thousands of these genes were common altered genes in both ovary and anther libraries. Gene ontology indicated the involvement of differentially expressed (DE) genes, responding to freezing stress, in metabolic and cellular processes. qRT-PCR analysis verified the expression pattern of eight genes randomly selected from the DE genes. In conclusion, the almond gene index assembled in this study and the reported DE genes can provide great insights on responses of almond and other Prunus species to abiotic stresses. The obtained results from current research would add to the limited available information on almond and Rosaceae. Besides, the findings would be very useful for comparative studies as the number of DE genes reported here is much higher than that of any previous reports in this plant.

  19. Black Alcoholism.

    Science.gov (United States)

    Watts, Thomas D.; Wright, Roosevelt

    1988-01-01

    Examines some aspects of the problem of alcoholism among Blacks, asserting that Black alcoholism can best be considered in an ecological, environmental, sociocultural, and public health context. Notes need for further research on alcoholism among Blacks and for action to reduce the problem of Black alcoholism. (NB)

  20. Efficient removal of Acid Green 25 dye from wastewater using activated Prunus Dulcis as biosorbent: Batch and column studies.

    Science.gov (United States)

    Jain, Suyog N; Gogate, Parag R

    2018-03-15

    Biosorbent synthesized from dead leaves of Prunus Dulcis with chemical activation during the synthesis was applied for the removal of Acid Green 25 dye from wastewater. The obtained biosorbent was characterized using Brunauer-Emmett-Teller analysis, Fourier transform-infrared spectroscopy and scanning electron microscopy measurements. It was demonstrated that alkali treatment during the synthesis significantly increased surface area of biosorbent from 67.205 to 426.346 m 2 /g. The effect of various operating parameters on dye removal was investigated in batch operation and optimum values of parameters were established as pH of 2, 14 g/L as the dose of natural biosorbent and 6 g/L as the dose of alkali treated biosorbent. Relative error values were determined to check fitting of obtained data to the different kinetic and isotherm models. It was established that pseudo-second order kinetic model and Langmuir isotherm fitted suitably to the obtained batch experimental data. Maximum biosorption capacity values were estimated as 22.68 and 50.79 mg/g for natural biosorbent and for alkali activated Prunus Dulcis, respectively. Adsorption was observed as endothermic and activation energy of 6.22 kJ/mol confirmed physical type of adsorption. Column experiments were also conducted to probe the effectiveness of biosorbent for practical applications in continuous operation. Breakthrough parameters were established by studying the effect of biosorbent height, flow rate of dye solution and initial dye concentration on the extent of dye removal. The maximum biosorption capacity under optimized conditions in the column operation was estimated as 28.57 mg/g. Thomas and Yoon-Nelson models were found to be suitably fitted to obtained column data. Reusability study carried out in batch and continuous column operations confirmed that synthesized biosorbent can be used repeatedly for dye removal from wastewater. Copyright © 2018 Elsevier Ltd. All rights reserved.

  1. Purification, identification and preliminary crystallographic studies of Pru du amandin, an allergenic protein from Prunus dulcis

    International Nuclear Information System (INIS)

    Gaur, Vineet; Sethi, Dhruv K.; Salunke, Dinakar M.

    2007-01-01

    The purification, identification, crystallization and preliminary crystallographic studies of an allergy-related protein, Pru du amandin, from P. dulcis nuts are reported. Food allergies appear to be one of the foremost causes of hypersensitivity reactions. Nut allergies account for most food allergies and are often permanent. The 360 kDa hexameric protein Pru du amandin, a known allergen, was purified from almonds (Prunus dulcis) by ammonium sulfate fractionation and ion-exchange chromatography. The protein was identified by a BLAST homology search against the nonredundant sequence database. Pru du amandin belongs to the 11S legumin family of seed storage proteins characterized by the presence of a cupin motif. Crystals were obtained by the hanging-drop vapour-diffusion method. The crystals belong to space group P4 1 (or P4 3 ), with unit-cell parameters a = b = 150.7, c = 164.9 Å

  2. Préparation et caractérisation d'un charbon actif à partir de la coquille d'amande (Prunus amygdalus amère

    Directory of Open Access Journals (Sweden)

    Trachi, M.

    2014-01-01

    Full Text Available Preparation and characterization of activated coal from bitter almond shells (Prunus amygdalus. Description of the subject. The present study concerns the preparation of activated coal (AC from bitter almond (Prunus amygdalus shells (BASh, a fruit that grows spontaneously in the Setif region (northeast Algeria. Obtaining and characterizing activated coal was the valorization method adopted here. Objectives. The aim of this study was to elucidate the feasibility of the chemical activation of BASh in order to obtain two types of activated coal (AC. Method. The two ACs were obtained from BASh by acid (CAa and basic (CAb activation. The final products were investigated for their different physicochemical characteristics: angle of repose, ash, differential screening calorimetry, etc. In the case of CAa, the modeling of the adsorption kinetic of methylene blue (MB, as well as of adsorption isotherms, was also performed. Results. The activation mode seemed to affect unequally the different physicochemical characteristics of both the obtained coals. On the other hand, the adsorption kinetics of the MB by CAa was correctly described by the pseudo-2nd-order model (R² = 0.999. Concerning the modeling of the adsorption isotherm, of the three models tested, the Freundlich model appeared to be the most appropriate to fit the experimental data (R2 = 0.898, mean relative error (MRE = 38.638 and root mean square error (RMSE = 1.039. In addition, the activated shell showed an interesting capacity for MB adsorption, estimated at 99.05%.

  3. Genome wide identification of chilling responsive microRNAs in Prunus persica

    Directory of Open Access Journals (Sweden)

    Barakat Abdelali

    2012-09-01

    Full Text Available Abstract Background MicroRNAs (miRNAs are small RNAs (sRNAs approximately 21 nucleotides in length that negatively control gene expression by cleaving or inhibiting the translation of target gene transcripts. Within this context, miRNAs and siRNAs are coming to the forefront as molecular mediators of gene regulation in plant responses to annual temperature cycling and cold stress. For this reason, we chose to identify and characterize the conserved and non-conserved miRNA component of peach (Prunus persica (L. Batsch focusing our efforts on both the recently released whole genome sequence of peach and sRNA transcriptome sequences from two tissues representing non-dormant leaves and dormant leaf buds. Conserved and non-conserved miRNAs, and their targets were identified. These sRNA resources were used to identify cold-responsive miRNAs whose gene targets co-localize with previously described QTLs for chilling requirement (CR. Results Analysis of 21 million peach sRNA reads allowed us to identify 157 and 230 conserved and non-conserved miRNA sequences. Among the non-conserved miRNAs, we identified 205 that seem to be specific to peach. Comparative genome analysis between peach and Arabidopsis showed that conserved miRNA families, with the exception of miR5021, are similar in size. Sixteen of these conserved miRNA families are deeply rooted in land plant phylogeny as they are present in mosses and/or lycophytes. Within the other conserved miRNA families, five families (miR1446, miR473, miR479, miR3629, and miR3627 were reported only in tree species (Populustrichocarpa, Citrus trifolia, and Prunus persica. Expression analysis identified several up-regulated or down-regulated miRNAs in winter buds versus young leaves. A search of the peach proteome allowed the prediction of target genes for most of the conserved miRNAs and a large fraction of non-conserved miRNAs. A fraction of predicted targets in peach have not been previously reported in other

  4. Immunocapture reverse transcription-polymerase chain reaction combined with nested PCR greatly increases the detection of Prunus necrotic ring spot virus in the peach.

    Science.gov (United States)

    Helguera, P R; Taborda, R; Docampo, D M; Ducasse, D A

    2001-06-01

    A detection system based on nested PCR after IC-RT-PCR (IC-RT-PCR-Nested PCR) was developed to improve indexing of Prunus necrotic ringspot virus in peach trees. Inhibitory effects and inconsistencies of the standard IC-RT-PCR were overcome by this approach. IC-RT-PCR-Nested PCR improved detection by three orders of magnitude compared with DAS-ELISA for the detection of PNRSV in leaves. Several different tissues were evaluated and equally consistent results were observed. The main advantages of the method are its consistency, high sensitivity and easy application in quarantine programs.

  5. Evaluación productiva, económica y social del agua de riego de durazno (Prunus persica L. Batsch) en Zacatecas (México)

    OpenAIRE

    José Luis Ríos-Flores; Miriam Torres-Moreno; José Ruiz-Torres; Marco Antonio Torres-Moreno; Jesús Enrique Cantú-Brito

    2015-01-01

    La escasez de agua limita la agricultura, por lo que su uso debe ser más eficiente en la producción de alimentos. El objetivo de este trabajo fue determinar la productividad económica, física y social del agua del cultivo de durazno (Prunus persica L. Batsch), en la región del Distrito de Desarrollo Rural 183 —correspondiente a Fresnillo, Zacatecas— para el ciclo 2012; se desarrollaron modelos matemáticos para estimar la productividad y eficiencia del agua. En Zacatecas se cosecharon 817 ha d...

  6. Black holes

    OpenAIRE

    Brügmann, B.; Ghez, A. M.; Greiner, J.

    2001-01-01

    Recent progress in black hole research is illustrated by three examples. We discuss the observational challenges that were met to show that a supermassive black hole exists at the center of our galaxy. Stellar-size black holes have been studied in x-ray binaries and microquasars. Finally, numerical simulations have become possible for the merger of black hole binaries.

  7. The phase structure of higher-dimensional black rings and black holes

    International Nuclear Information System (INIS)

    Emparan, Roberto; Harmark, Troels; Niarchos, Vasilis; Obers, Niels A.; RodrIguez, Maria J.

    2007-01-01

    We construct an approximate solution for an asymptotically flat, neutral, thin rotating black ring in any dimension D ≥ 5 by matching the near-horizon solution for a bent boosted black string, to a linearized gravity solution away from the horizon. The rotating black ring solution has a regular horizon of topology S 1 x S D-3 and incorporates the balancing condition of the ring as a zero-tension condition. For D = 5 our method reproduces the thin ring limit of the exact black ring solution. For D ≥ 6 we show that the black ring has a higher entropy than the Myers-Perry black hole in the ultra-spinning regime. By exploiting the correspondence between ultra-spinning black holes and black membranes on a two-torus, we take steps towards qualitatively completing the phase diagram of rotating blackfolds with a single angular momentum. We are led to propose a connection between MP black holes and black rings, and between MP black holes and black Saturns, through merger transitions involving two kinds of 'pinched' black holes. More generally, the analogy suggests an infinite number of pinched black holes of spherical topology leading to a complicated pattern of connections and mergers between phases

  8. On the Charter Question: Black Marxism and Black Nationalism

    Science.gov (United States)

    Stern, Mark; Hussain, Khuram

    2015-01-01

    This article brings two black intellectual traditions to bear on the question of charter schools: black Marxism and black nationalism. The authors examine the theoretical and rhetorical devices used to talk about charters schools by focusing on how notions of "black liberation" are deployed by the charter movement, and to what end. The…

  9. "Be Real Black for Me": Imagining BlackCrit in Education

    Science.gov (United States)

    Dumas, Michael J.; ross, kihana miraya

    2016-01-01

    The authors put forward a theorization of a Black Critical Theory, or what might be called BlackCrit, within, and in response to, Critical Race Theory, and then outline ways that BlackCrit in education helps us to more incisively analyze how the specificity of (anti)blackness matters in explaining how Black bodies become marginalized, disregarded,…

  10. Caracterización con RAMs de la colección de durazno (Prunus persica L. Batsch existente en la Universidad Pedagógica y Tecnológica de Colombia

    Directory of Open Access Journals (Sweden)

    Ana Cruz Morillo Coronado

    2014-12-01

    Full Text Available Utilizando ocho cebadores Microsatélites Amplificados al Azar (RAMs fue evaluada la diversidad genética de 31 materiales de Prunus de la colección de caducifolios existente en la Universidad Pedagógica y Tecnológica de Colombia. Se generaron un total de 121 amplicones con pesos moleculares entre 260 y 1000 Kb. Con un coeficiente de similitud de 0.75, se formaron tres grupos, de acuerdo principalmente con las características del fruto, encontrando en el grupo 2 las variedades de importancia económica. El número de loci polimórficos varió entre 5 y 16 para los cebadores GT y CCA, respectivamente. El valor promedio de heterocigosidad fue de 0.22, más bajo que los encontrados en otros estudios de diversidad genética en el género Prunus; por tanto, se deben buscar estrategias para incrementar la variabilidad genética de la colección como la introducción de nuevos materiales o híbridos interespecíficos. La técnica RAMs es útil para evaluar la diversidad genética en especies caducifolias.

  11. Black hole astrophysics

    International Nuclear Information System (INIS)

    Blandford, R.D.; Thorne, K.S.

    1979-01-01

    Following an introductory section, the subject is discussed under the headings: on the character of research in black hole astrophysics; isolated holes produced by collapse of normal stars; black holes in binary systems; black holes in globular clusters; black holes in quasars and active galactic nuclei; primordial black holes; concluding remarks on the present state of research in black hole astrophysics. (U.K.)

  12. Almond (Prunus dulcis (Mill.) D.A. Webb) skins as a potential source of bioactive polyphenols.

    Science.gov (United States)

    Monagas, Maria; Garrido, Ignacio; Lebrón-Aguilar, Rosa; Bartolome, Begoña; Gómez-Cordovés, Carmen

    2007-10-17

    An exhaustive study of the phenolic composition of almond ( Prunus dulcis (Mill.) D.A. Webb) skins was carried out in order to evaluate their potential application as a functional food ingredient. Using the HPLC-DAD/ESI-MS technique, a total of 33 compounds corresponding to flavanols, flavonols, dihydroflavonols and flavanones, and other nonflavonoid compounds were identified. Peaks corresponding to another 23 structure-related compounds were also detected. MALDI-TOF MS was applied to characterize almond skin proanthocyanidins, revealing the existence of a series of A- and B-type procyanidins and propelargonidins up to heptamers, and A- and B-type prodelphinidins up to hexamers. Flavanols and flavonol glycosides were the most abundant phenolic compounds in almond skins, representing up to 38-57% and 14-35% of the total quantified phenolics, respectively. Due to their antioxidant properties, measured as oxygen-radical absorbance capacity (ORAC) at 0.398-0.500 mmol Trolox/g, almond skins can be considered as a value-added byproduct for elaborating dietary antioxidant ingredients.

  13. UTILIZACION DE ISOENZIMAS DE EXTRACTOS DE HOJAS EN LA CARACTERIZACION DE CULTIVARES DE DURAZNERO (Prunus persica (L Batsch THE USE OF ISOZYME LEAF EXTRACTS IN THE CHARACTERIZATION OF PEACH CULTIVARS (Prunus persica L Batsch

    Directory of Open Access Journals (Sweden)

    HECTOR ABEL ALTUBE

    2001-08-01

    Full Text Available La caracterización de cultivares de duraznero (Prunus persica (L Batsch se hace por medio de la descripción de caracteres agronómicos y morfológicos codificados por organizaciones internacionales, los cuales están fuertemente influenciados por el ambiente. Se han buscado métodos alternativos de caracterización y las isoenzimas han sido utilizadas por su independencia de las condiciones del ambiente, además de identificar individuos en etapas tempranas de su desarrollo. El objetivo del presente estudio es caracterizar cultivares de duraznero mediante el análisis isoenzimático de catecol oxidasas, fosfatasas ácidas, esterazas y peroxidazos en extractos de hojas. Los cultivares de duraznero analizados presentaron bajo polimorfismo isoenzimático, las esterazas caracterizaron diez cultivares, las catecol oxidasas un cultivar agrupándose el resto en cinco modelos, las fosfatasas ácidas caracterizaron dos cultivares agrupándose los otros en siete modelos y las peroxidazos formaron tres grupos. Ello puede explicarse ya que el duraznero es una especie autofértil y presenta una base genética muy reducida. Los evidentes límites discriminatorios de este tipo de análisis hacen que su aporte sea sólo complementario a los métodos de los caracteres agronómicos y morfológicos.Characterization of peach cultivars (Prunus persica (L Batsh was made by description of agronomical and morphological characters codified from international organizations, which are strongly affected by environmental conditions. Alternative methods of characterization have been searched, and isoenzymes have been used as independent of environmental conditions in addition to identify some individuals in early stages of development. The goal of this study is the peach cultivars characterization by isoenzymatic analysis of catecol oxidases, acid phosphatases, esterases and peroxidases within the leaf extracts. The peach cultivars analyzed have showed low isoenzymatic

  14. Biosorption of eriochrome black t and astrazon fggl blue using almond and cotton seed oil cake biomass in a batch mode

    International Nuclear Information System (INIS)

    Safa, Y.

    2014-01-01

    In the present research study, the biosorption of Eriochrome Black T (EBT) and Astrazon FGGL blue (A-FGGL) onto novel biomasses Almond (Prunus dulcis) oil cake and Cotton seed oil cake respectively was investigated in the batch mode using different process parameters like pH, particle size, biosorbent dose, initial dye concentration, contact time and temperature. Maximum biosorption capacity was observed at pH 3 for EBT onto almond oil cake and pH2 for Astrazon FGGL blue onto cotton seed oil cake.The biosorption capacity was efficient at the smallest particle size of biosorbent. The amount of dye sorbed (mg/g) decreased with the decrease in biosorbent dose and increased with increase in initial dye concentration and temperature. Optimum contact time for equilibrium to achieve was found to be 120 and 180 minutes for EBT and A-FGGL blue, respectively. The Langmuir isotherm model was best fitted to experimental data. The biosorption followed the pseudo-second order kinetic model suggesting a chemisorption mechanism. The positive value of deltaH showed the endothermic nature of the process. In this research, the influence of electrolytes, heavy metals and surfactants on the removal of dyes was also examined. (author)

  15. Purification, identification and preliminary crystallographic studies of Pru du amandin, an allergenic protein from Prunus dulcis

    Energy Technology Data Exchange (ETDEWEB)

    Gaur, Vineet; Sethi, Dhruv K.; Salunke, Dinakar M., E-mail: dinakar@nii.res.in [National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067 (India)

    2008-01-01

    The purification, identification, crystallization and preliminary crystallographic studies of an allergy-related protein, Pru du amandin, from P. dulcis nuts are reported. Food allergies appear to be one of the foremost causes of hypersensitivity reactions. Nut allergies account for most food allergies and are often permanent. The 360 kDa hexameric protein Pru du amandin, a known allergen, was purified from almonds (Prunus dulcis) by ammonium sulfate fractionation and ion-exchange chromatography. The protein was identified by a BLAST homology search against the nonredundant sequence database. Pru du amandin belongs to the 11S legumin family of seed storage proteins characterized by the presence of a cupin motif. Crystals were obtained by the hanging-drop vapour-diffusion method. The crystals belong to space group P4{sub 1} (or P4{sub 3}), with unit-cell parameters a = b = 150.7, c = 164.9 Å.

  16. Contemporary Black Theatre.

    Science.gov (United States)

    Thomas, Pearl

    The distinguishable black theatre in America, mirroring a distinguishable black experience, is an artistic product which demands audience involvement. Both the Afro-American oral tradition and the art of gesture are integral aspects of black theatre. In addition, the tragedy found black theatre is not tragedy in the classic sense, as blacks feel…

  17. Comparative effects of prolonged administration of cyanide, thiocyanate and chokecherry (Prunus virginiana) to goats.

    Science.gov (United States)

    Soto-Blanco, B; Stegelmeier, B L; Pfister, J A; Gardner, D R; Panter, K E

    2008-04-01

    The aim of the present study was to determine and compare the clinical, hematological, biochemical and histopathological changes induced by cyanide, thiocyanate and chokecherry (Prunus virginiana) in goats. Sixteen Boer-Spanish cross-bred female goats were divided into four treatment groups: (1) control, (2) potassium cyanide (KCN) at 3.8 mg kg(-1) day(-1), (3) potassium thiocyanate (KSCN) at 4.5 mg kg(-1) day(-1) and (4) ground frozen chokecherry leaves and flowers at a target dose of 2.5 mg HCN kg(-1) day(-1), all for 4 weeks. Clinical signs were observed in two goats treated with chokecherry. Only sporadic changes were found in the hematological and blood chemical panel. Goats treated with chokecherry and thiocyanate had an increased number of vacuoles in the colloid of thyroid glands. Spongiosis and spheroids were found in the mesencephalon from goats treated with KCN and chokecherry. These findings suggest the thyroid lesions can be attributed to thiocyanate, whereas the effects on the nervous system were most likely caused by cyanide.

  18. Caged black holes: Black holes in compactified spacetimes. I. Theory

    International Nuclear Information System (INIS)

    Kol, Barak; Sorkin, Evgeny; Piran, Tsvi

    2004-01-01

    In backgrounds with compact dimensions there may exist several phases of black objects including a black hole and a black string. The phase transition between them raises questions and touches on fundamental issues such as topology change, uniqueness, and cosmic censorship. No analytic solution is known for the black hole, and moreover one can expect approximate solutions only for very small black holes, while phase transition physics happens when the black hole is large. Hence we turn to numerical solutions. Here some theoretical background to the numerical analysis is given, while the results will appear in a subsequent paper. The goals for a numerical analysis are set. The scalar charge and tension along the compact dimension are defined and used as improved order parameters which put both the black hole and the black string at finite values on the phase diagram. The predictions for small black holes are presented. The differential and the integrated forms of the first law are derived, and the latter (Smarr's formula) can be used to estimate the 'overall numerical error'. Field asymptotics and expressions for physical quantities in terms of the numerical values are supplied. The techniques include the 'method of equivalent charges', free energy, dimensional reduction, and analytic perturbation for small black holes

  19. Link between defoliation and light treatments on root vitality of five understory shrubs with different resistance to insect herbivory.

    Science.gov (United States)

    Karolewski, Piotr; Zadworny, Marcin; Mucha, Joanna; Napierała-Filipiak, Anna; Oleksyn, Jacek

    2010-08-01

    Understory shrubs are frequently attacked by insect herbivores. However, very little is known regarding possible interactions between light condition, defoliation (D) and fine root vitality (% live roots) and metabolic activity, and whether different plant strategies (compensation, trade-off and equilibrium) to defoliation depend on individual species light requirements. To explore the response of roots to such conditions, an experiment was established in which we experimentally removed 50% of leaves in 1-year-old seedlings of Sambucus nigra, Cornus sanguinea, Prunus serotina, Frangula alnus and Corylus avellana grown in 15% and full sunlight. On average, defoliation leads to a 15% reduction in fine root (vitality (% live roots). However, a statistically significant reduction in root vitality after defoliation was detected only in those species that are less herbivorized in nature (48% in S. nigra and 5% in C. sanguinea). On average, shade conditions (L) resulted in 18% decline in root vitality, and the effects of defoliation were also 22% higher than for plants grown in full light. Root vitality in both treatments (D and L) was significantly correlated with their dry mass, concentration of total phenol (TPh) and carbon to nitrogen ratio, and negatively correlated with nitrogen, soluble carbohydrates, starch and total non-structural carbohydrates (TNC). To a large extent, root vitality and chemistry varied by species. Higher root vitality was related to higher concentrations of phenolics, more than to N and TNC concentrations. Concentrations of phenolics also differed significantly between defoliated plants and controls. However, in defoliated plants, an increase in TPh was observed only in two species, which belong to two different groups in light requirements and susceptibility to insect grazing (C. sanguinea and P. serotina). This study indicated that higher vitality of roots occurred in species that are characterized by higher insect defoliation under natural

  20. Primary black holes

    International Nuclear Information System (INIS)

    Novikov, I.; Polnarev, A.

    1981-01-01

    Proves are searched for of the formation of the so-called primary black holes at the very origin of the universe. The black holes would weigh less than 10 13 kg. The formation of a primary black hole is conditional on strong fluctuations of the gravitational field corresponding roughly to a half of the fluctuation maximally permissible by the general relativity theory. Only big fluctuations of the gravitational field can overcome the forces of the hot gas pressure and compress the originally expanding matter into a black hole. Low-mass black holes have a temperature exceeding that of the black holes formed from stars. A quantum process of particle formation, the so-called evaporation takes place in the strong gravitational field of a black hole. The lower the mass of the black hole, the shorter the evaporation time. The analyses of processes taking place during the evaporation of low-mass primary black holes show that only a very small proportion of the total mass of the matter in the universe could turn into primary black holes. (M.D.)

  1. 77 FR 70423 - Black Bear Hydro Partners, LLC and Black Bear Development Holdings, LLC and Black Bear SO, LLC...

    Science.gov (United States)

    2012-11-26

    ... Bear Hydro Partners, LLC and Black Bear Development Holdings, LLC and Black Bear SO, LLC; Notice of..., 2012, Black Bear Hydro Partners, LLC, sole licensee (transferor) and Black Bear Development Holdings, LLC and Black Bear SO, LLC (transferees) filed an application for the partial the transfer of licenses...

  2. Impacts of Military Training and Land Management on Threatened and Endangered Species in the Southeastern Fall Line Sandhills Communities

    Science.gov (United States)

    2009-05-01

    0.01 a 0 0.16 a 0.03 a 0.11 a Diospyros virginiana (persimmon) * 0 0 0.01 a 0 * 0 0 Nyssa sylvatica (black gum) 0 * 0 0.05 a 0 0.05 a 0 0...0 0 0.02 b 0 0.01 b 0.16 a 0 Prunus spp. (cherry) 0 0.42 a 0 * 0.02 b 0.01 b 0 0.02 b Sassafras albidum (sassafras) 0 * 0 * * 0 0 0...disturbance treatments were composed primarily of longleaf pine, turkey oak, sand post oak, bluejack oak, persimmon (Diospyros virginiana ), black gum (Nyssa

  3. Phase transition for black holes with scalar hair and topological black holes

    International Nuclear Information System (INIS)

    Myung, Yun Soo

    2008-01-01

    We study phase transitions between black holes with scalar hair and topological black holes in asymptotically anti-de Sitter spacetimes. As the ground state solutions, we introduce the non-rotating BTZ black hole in three dimensions and topological black hole with hyperbolic horizon in four dimensions. For the temperature matching only, we show that the phase transition between black hole with scalar hair (Martinez-Troncoso-Zanelli black hole) and topological black hole is second-order by using differences between two free energies. However, we do not identify what order of the phase transition between scalar and non-rotating BTZ black holes occurs in three dimensions, although there exists a possible decay of scalar black hole to non-rotating BTZ black hole

  4. From binary black hole simulation to triple black hole simulation

    International Nuclear Information System (INIS)

    Bai Shan; Cao Zhoujian; Han, Wen-Biao; Lin, Chun-Yu; Yo, Hwei-Jang; Yu, Jui-Ping

    2011-01-01

    Black hole systems are among the most promising sources for a gravitational wave detection project. Now, China is planning to construct a space-based laser interferometric detector as a follow-on mission of LISA in the near future. Aiming to provide some theoretical support to this detection project on the numerical relativity side, we focus on black hole systems simulation in this work. Considering the globular galaxy, multiple black hole systems also likely to exist in our universe and play a role as a source for the gravitational wave detector we are considering. We will give a progress report in this paper on our black hole system simulation. More specifically, we will present triple black hole simulation together with binary black hole simulation. On triple black hole simulations, one novel perturbational method is proposed.

  5. Vertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling.

    Science.gov (United States)

    Amari, Khalid; Burgos, Lorenzo; Pallás, Vicente; Sánchez-Pina, Maria Amelia

    2009-07-01

    The aim of this work was to follow Prunus necrotic ringspot virus (PNRSV) infection in apricot reproductive tissues and transmission of the virus to the next generation. For this, an analysis of viral distribution in apricot reproductive organs was carried out at different developmental stages. PNRSV was detected in reproductive tissues during gametogenesis. The virus was always present in the nucellus and, in some cases, in the embryo sac. Studies within infected seeds at the embryo globular stage revealed that PNRSV infects all parts of the seed, including embryo, endosperm and testa. In the torpedo and bent cotyledon developmental stages, high concentrations of the virus were detected in the testa and endosperm. At seed maturity, PNRSV accumulated slightly more in the embryo than in the cotyledons. In situ hybridization showed the presence of PNRSV RNA in embryos obtained following hand-pollination of virus-free pistils with infected pollen. Interestingly, tissue-printing from fruits obtained from these pistils showed viral RNA in the periphery of the fruits, whereas crosses between infected pistils and infected pollen resulted in a total invasion of the fruits. Taken together, these results shed light on the vertical transmission of PNRSV from gametes to seedlings.

  6. Recent advancements to study flowering time in almond and other Prunus species.

    Science.gov (United States)

    Sánchez-Pérez, Raquel; Del Cueto, Jorge; Dicenta, Federico; Martínez-Gómez, Pedro

    2014-01-01

    Flowering time is an important agronomic trait in almond since it is decisive to avoid the late frosts that affect production in early flowering cultivars. Evaluation of this complex trait is a long process because of the prolonged juvenile period of trees and the influence of environmental conditions affecting gene expression year by year. Consequently, flowering time has to be studied for several years to have statistical significant results. This trait is the result of the interaction between chilling and heat requirements. Flowering time is a polygenic trait with high heritability, although a major gene Late blooming (Lb) was described in "Tardy Nonpareil." Molecular studies at DNA level confirmed this polygenic nature identifying several genome regions (Quantitative Trait Loci, QTL) involved. Studies about regulation of gene expression are scarcer although several transcription factors have been described as responsible for flowering time. From the metabolomic point of view, the integrated analysis of the mechanisms of accumulation of cyanogenic glucosides and flowering regulation through transcription factors open new possibilities in the analysis of this complex trait in almond and in other Prunus species (apricot, cherry, peach, plum). New opportunities are arising from the integration of recent advancements including phenotypic, genetic, genomic, transcriptomic, and metabolomics studies from the beginning of dormancy until flowering.

  7. Surface geometry of 5D black holes and black rings

    International Nuclear Information System (INIS)

    Frolov, Valeri P.; Goswami, Rituparno

    2007-01-01

    We discuss geometrical properties of the horizon surface of five-dimensional rotating black holes and black rings. Geometrical invariants characterizing these 3D geometries are calculated. We obtain a global embedding of the 5D rotating black horizon surface into a flat space. We also describe the Kaluza-Klein reduction of the black ring solution (along the direction of its rotation) which, though it is nakedly singular, relates this solution to the 4D metric of a static black hole distorted by the presence of external scalar (dilaton) and vector ('electromagnetic') fields. The properties of the reduced black hole horizon and its embedding in E 3 are briefly discussed

  8. Black Holes

    OpenAIRE

    Townsend, P. K.

    1997-01-01

    This paper is concerned with several not-quantum aspects of black holes, with emphasis on theoretical and mathematical issues related to numerical modeling of black hole space-times. Part of the material has a review character, but some new results or proposals are also presented. We review the experimental evidence for existence of black holes. We propose a definition of black hole region for any theory governed by a symmetric hyperbolic system of equations. Our definition reproduces the usu...

  9. Safety and efficacy of a novel Prunus domestica extract (Sitoprin, CR002) on testosterone-induced benign prostatic hyperplasia (BPH) in male Wistar rats.

    Science.gov (United States)

    Swaroop, Anand; Bagchi, Manashi; Kumar, Pawan; Preuss, Harry G; Bagchi, Debasis

    2015-01-01

    The efficacy of a novel Prunus domestica bark extract (Sitoprin, CR002) was investigated on testosterone propionate (TP)-induced benign prostatic hyperplasia (BPH) in male Wistar rats. BPH was induced by daily subcutaneous administration of TP (3.0 mg/kg) over a period of 15 days (interim sacrifice group) and for an additional 21 days (terminal sacrifice group). We evaluated the dose-dependent efficacy (0, 50, 100 and 200 mg/kg body weight/day) of CR002 and a control group against BPH, and compared with a reference standard Prunus africana extract (CR001). Extensive clinical examinations were carried out on days 1, 7, 14, 21, 28 and 35 of treatment period to determine the onset, duration and severity of clinical signs. Clinical pathology, hematology, biochemistry and histopathology were performed on days 15 and 35, prior to necropsy. Animals were fasted overnight prior to blood collection. Prostate glands and tissues were examined. On day 36, histopathology of ventral prostrate of control rats demonstrates single layer of columnar mucin secreting epithelial cells along with a lumen occupied with eosinophilic secretion. In contrast, CR002 and CR001 groups (100 and 200 mg/kg/day) exhibited no hyperplasia and proliferation of epithelial cells. Prostate histopathology of these treated groups was comparable with control rats. The hyperplasia and hypertrophy of prostrate was reduced to single-layered cell indicating the efficacy of CR002 and CR001. Overall, results demonstrate that CR002 exhibits therapeutic efficacy/activity in TP-induced BPH in rats, which is comparable to CR001.

  10. Black silicon solar cells with black bus-bar strings

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Tang, Peter Torben; Mizushima, Io

    2016-01-01

    We present the combination of black silicon texturing and blackened bus-bar strings as a potential method for obtaining all-black solar panels, while using conventional, front-contacted solar cells. Black silicon was realized by maskless reactive ion etching resulting in total, average reflectance...... below 0.5% across a 156x156 mm2 silicon wafer. Four different methods to obtain blackened bus-bar strings were compared with respect to reflectance, and two of these methods (i.e., oxidized copper and etched solder) were used to fabricate functional allblack solar 9-cell panels. The black bus-bars (e.......g., by oxidized copper) have a reflectance below 3% in the entire visible wavelength range. The combination of black silicon cells and blackened bus-bars results in aesthetic, all-black panels based on conventional, front-contacted solar cells without compromising efficiency....

  11. Distortion of Schwarzschild-anti-de Sitter black holes to black strings

    International Nuclear Information System (INIS)

    Tomimatsu, Akira

    2005-01-01

    Motivated by the existence of black holes with various topologies in four-dimensional spacetimes with a negative cosmological constant, we study axisymmetric static solutions describing any large distortions of Schwarzschild-anti-de Sitter black holes parametrized by the mass m. Under the approximation such that m is much larger than the anti-de Sitter radius, it is found that a cylindrically symmetric black string is obtained as a special limit of distorted spherical black holes. Such a prolonged distortion of the event horizon connecting a Schwarzschild-anti-de Sitter black hole to a black string is allowed without violating both the usual black hole thermodynamics and the hoop conjecture for the horizon circumference

  12. Low-mass black holes as the remnants of primordial black hole formation.

    Science.gov (United States)

    Greene, Jenny E

    2012-01-01

    Bridging the gap between the approximately ten solar mass 'stellar mass' black holes and the 'supermassive' black holes of millions to billions of solar masses are the elusive 'intermediate-mass' black holes. Their discovery is key to understanding whether supermassive black holes can grow from stellar-mass black holes or whether a more exotic process accelerated their growth soon after the Big Bang. Currently, tentative evidence suggests that the progenitors of supermassive black holes were formed as ∼10(4)-10(5) M(⊙) black holes via the direct collapse of gas. Ongoing searches for intermediate-mass black holes at galaxy centres will help shed light on this formation mechanism.

  13. Soil magnetic susceptibility: A quantitative proxy of soil drainage for use in ecological restoration

    Science.gov (United States)

    Grimley, D.A.; Wang, J.-S.; Liebert, D.A.; Dawson, J.O.

    2008-01-01

    Flooded, saturated, or poorly drained soils are commonly anaerobic, leading to microbially induced magnetite/maghemite dissolution and decreased soil magnetic susceptibility (MS). Thus, MS is considerably higher in well-drained soils (MS typically 40-80 ?? 10-5 standard international [SI]) compared to poorly drained soils (MS typically 10-25 ?? 10-5 SI) in Illinois, other soil-forming factors being equal. Following calibration to standard soil probings, MS values can be used to rapidly and precisely delineate hydric from nonhydric soils in areas with relatively uniform parent material. Furthermore, soil MS has a moderate to strong association with individual tree species' distribution across soil moisture regimes, correlating inversely with independently reported rankings of a tree species' flood tolerance. Soil MS mapping can thus provide a simple, rapid, and quantitative means for precisely guiding reforestation with respect to plant species' adaptations to soil drainage classes. For instance, in native woodlands of east-central Illinois, Quercus alba , Prunus serotina, and Liriodendron tulipifera predominantly occur in moderately well-drained soils (MS 40-60 ?? 10-5 SI), whereas Acer saccharinum, Carya laciniosa, and Fraxinus pennsylvanica predominantly occur in poorly drained soils (MS Urbana, IL, U.S.A.). Through use of soil MS maps calibrated to soil drainage class and native vegetation occurrence, restoration efforts can be conducted more successfully and species distributions more accurately reconstructed at the microecosystem level. ?? 2008 Society for Ecological Restoration International.

  14. Sweet almond (Prunus amygdalus “dulcis” seeds as a potential feedstock for Nigerian Biodiesel Automotive Project

    Directory of Open Access Journals (Sweden)

    Solomon Giwa

    2014-01-01

    Full Text Available This work presents sweet almond (Prunus amygdalus “dulcis” seed oil (SASO as a non-conventional feedstock for the preparation of biodiesel in Nigeria, rather than the traditional oils of palm, groundnut and palm kernel. SASO was extracted via the solvent method, pretreated to reduce the acid value, and transesterified using methanol (solvent and sodium hydroxide (catalyst. The oil content and acid value of SASO were 51.45 ± 3.92% and 1.07 mg KOH/g, respectively. The fatty acid composition of SASO reveals the predominance of oleic acid (69.7%, linoleic acid (18.2% and palmitic acid (9.3%. Specific fuel properties of sweet almond oil methyl esters (SAOME were determined using standard test methods and were found to satisfy both EN 14214 and ASTM D6751 biodiesel standards; the cold flow properties were particularly outstanding (cloud point; -3ºC and pour point; -9ºC. SASO appears to offer great promise as a potential feedstock for biodiesel production in Nigeria.

  15. Effect of Temperature and Moisture on the Development of Concealed Damage in Raw Almonds (Prunus dulcis).

    Science.gov (United States)

    Rogel-Castillo, Cristian; Zuskov, David; Chan, Bronte Lee; Lee, Jihyun; Huang, Guangwei; Mitchell, Alyson E

    2015-09-23

    Concealed damage (CD) is a brown discoloration of nutmeat that appears only after kernels are treated with moderate heat (e.g., roasting). Identifying factors that promote CD in almonds is of significant interest to the nut industry. Herein, the effect of temperature (35 and 45 °C) and moisture (almonds (Prunus dulcis var. Nonpareil) was studied using HS-SPME-GC/MS. A CIE LCh colorimetric method was developed to identify raw almonds with CD. A significant increase in CD was demonstrated in almonds exposed to moisture (8% kernel moisture content) at 45 °C as compared to 35 °C. Elevated levels of volatiles related to lipid peroxidation and amino acid degradation were observed in almonds with CD. These results suggest that postharvest moisture exposure resulting in an internal kernel moisture ≥ 8% is a key factor in the development of CD in raw almonds and that CD is accelerated by temperature.

  16. Black Culture

    Directory of Open Access Journals (Sweden)

    Angela Khristin Brown

    2013-07-01

    Full Text Available The migration of blacks in North America through slavery became united.  The population of blacks past downs a tradition of artist through art to native born citizens. The art tradition involved telling stories to each generation in black families. The black culture elevated by tradition created hope to determine their personal freedom to escape from poverty of enslavement and to establish a way of life through tradition. A way of personal freedoms was through getting a good education that lead to a better foundation and a better way of life.

  17. Magnetized black holes and black rings in the higher dimensional dilaton gravity

    International Nuclear Information System (INIS)

    Yazadjiev, Stoytcho S.

    2006-01-01

    In this paper we consider magnetized black holes and black rings in the higher dimensional dilaton gravity. Our study is based on exact solutions generated by applying a Harrison transformation to known asymptotically flat black hole and black ring solutions in higher dimensional spacetimes. The explicit solutions include the magnetized version of the higher dimensional Schwarzschild-Tangherlini black holes, Myers-Perry black holes, and five-dimensional (dipole) black rings. The basic physical quantities of the magnetized objects are calculated. We also discuss some properties of the solutions and their thermodynamics. The ultrarelativistic limits of the magnetized solutions are briefly discussed and an explicit example is given for the D-dimensional magnetized Schwarzschild-Tangherlini black holes

  18. The effect of the time of budding of mahaleb cherry (Prunus mahaleb L. seedlings on the quality of maiden trees of sour cherry (Prunus cerasus L. 'Łutówka'

    Directory of Open Access Journals (Sweden)

    Piotr Baryła

    2012-12-01

    Full Text Available The present study was conducted at the Felin Experi- mental Farm, belonging to the University of Life Sciences in Lublin, during the period 2005–2008. The experimental material consisted of maiden trees of sour cherry 'Łutówka' budded on seedlings of mahaleb cherry (Prunus mahaleb L. of unknown origin. The experiment evaluated the effect of four budding times: 15 July, 1 August, 15 August, and 1 September, on the quality of cherry trees in a nursery. The mean for the three years showed that budding time did not have a significant effect on the quality of cherry trees in the nursery. It was observed that the budding of mahaleb cherry performed on the two August dates (1st and 15th had a more beneficial effect on the growth and branching of trees than the budding done on 15 July and 1 September. The quality of maiden cherry trees 'Łutówka' in the nursery was primarily dependent on weather conditions in a given growing season, which is evidenced by the significant differences between production cycles, high variation in the quantitative results in individual years, and the absence of significant differences in the mean for 2006–2008.

  19. Recreational Appendix Report, Elm Fork Flood Control Project, Dallas and Denton Counties, Texas.

    Science.gov (United States)

    1973-05-01

    Juniperus virginiana 2. Willow Salix nigra 3. Cottonwood Populus deltoides 4. Black Walnut Juglans migra 5. Pecan Carya illinoensis 6. Bur Oak...Maclura pomifera 12. Red Mulberry Morus rubra 13. Sycamore Platanus occidentailis 14. Red Haw Crataegus, sps. 15. Wild Plum Prunus mexicana 16. Mesquite...also provides a habitat for a number of mammals including: 1. Opossum Didelphis virginiana 2. Shrews Blarina brevicuada and Cryptotis parva 3. Raccoon

  20. The Black Lives Matter Movement and Historically Black Colleges and Universities

    Science.gov (United States)

    Gasman, Marybeth

    2017-01-01

    This article looks at the Black Lives Matter Movement and Historically Black Colleges and Universities. Historically Black Colleges and Universities (HBCUs) continue to play an important role in society. However, what the Black Lives Matter movement shows consistently is that predominantly White institutions need to change, to step up and embrace…

  1. Enantioselective Synthesis of Various Cyanohydrins Using Covalently Immobilized Preparations of Hydroxynitrile Lyase from Prunus dulcis.

    Science.gov (United States)

    Alagöz, Dilek; Tükel, S Seyhan; Yildirim, Deniz

    2015-11-01

    The carrier-based and carrier-free (cross-linked enzyme aggregate) covalent immobilizations of Prunus dulcis hydroxynitrile lyase were investigated. The immobilized preparations were tested for enantioselective carbon-carbon bond formation activity in the biphasic medium. Of the tested preparations, only cross-linked enzyme aggregate of P. dulcis hydroxynitrile lyase (PdHNL-CLEA) achieved the synthesis of (R)-mandelonitrile with 93% yield and 99% enantiopurity. PdHNL-CLEA was also used in the synthesis of various (R)-cyanohydrins from corresponding aldehydes/ketones and hydrocyanic acid. When 4-methoxybenzaldehyde, 4-methyl benzaldehyde, and 4-hydroxybenzaldehyde were used as substrates, the yield-enantiomeric excess of corresponding (R)-cyanohydrins were obtained as 95-95, 85-79, and 2-25%, respectively, after 96 h at pH 4.0 and 5 °C. For acetophenone, 4-fluoroacetophenone, 4-chloroacetophenone, 4-bromoacetophenone, and 4-iodoacetophenone, the yield-enantiomeric excess of corresponding (R)-cyanohydrins were 1-99, 20-84, 11-95, 5-99, and 3-24%, respectively at the same conditions. The results demonstrate PdHNL-CLEA can be effectively used in the synthesis of (R)-mandelonitrile.

  2. Pollination Requirements of Almond (Prunus dulcis): Combining Laboratory and Field Experiments.

    Science.gov (United States)

    Henselek, Yuki; Eilers, Elisabeth J; Kremen, Claire; Hendrix, Stephen D; Klein, Alexandra-Maria

    2018-03-08

    Almond (Prunus dulcis (Mill.) D. A. Webb; Rosales: Rosaceae) is a cash crop with an estimated global value of over seven billion U.S. dollars annually and commercial varieties are highly dependent on insect pollination. Therefore, the understanding of basic pollination requirements of the main varieties including pollination efficiency of honey bees (Apis mellifera, Linnaeus, Hymenoptera: Apidae) and wild pollinators is essential for almond production. We first conducted two lab experiments to examine the threshold number of pollen grains needed for successful pollination and to determine if varietal identity or diversity promotes fruit set and weight. Further, we examined stigma and ovules of flowers visited by Apis and non-Apis pollinators in the field to study the proportion of almond to non-almond pollen grains deposited, visitation time per flower visit, and tube set. Results indicate that the threshold for successful fertilization is around 60 pollen grains, but pollen can be from any compatible variety as neither pollen varietal identity nor diversity enhanced fruit set or weight. Andrena cerasifolii Cockerell (Hymenoptera: Andrenidae) was a more effective pollinator on a per single visit basis than Apis and syrphid flies. Nevertheless, Apis was more efficient than A. cerasifolii and syrphid flies as they spent less time on a flower during a single visit. Hence, planting with two compatible varieties and managing for both Apis and non-Apis pollinators is likely to be an optimal strategy for farmers to secure high and stable pollination success.

  3. Black hole levitron

    International Nuclear Information System (INIS)

    Arsiwalla, Xerxes D.; Verlinde, Erik P.

    2010-01-01

    We study the problem of spatially stabilizing four dimensional extremal black holes in background electric/magnetic fields. Whilst looking for stationary stable solutions describing black holes placed in external fields we find that taking a continuum limit of Denef et al.'s multicenter supersymmetric black hole solutions provides a supergravity description of such backgrounds within which a black hole can be trapped within a confined volume. This construction is realized by solving for a levitating black hole over a magnetic dipole base. We comment on how such a construction is akin to a mechanical levitron.

  4. Black Holes

    OpenAIRE

    Horowitz, Gary T.; Teukolsky, Saul A.

    1998-01-01

    Black holes are among the most intriguing objects in modern physics. Their influence ranges from powering quasars and other active galactic nuclei, to providing key insights into quantum gravity. We review the observational evidence for black holes, and briefly discuss some of their properties. We also describe some recent developments involving cosmic censorship and the statistical origin of black hole entropy.

  5. Search for black holes

    International Nuclear Information System (INIS)

    Cherepashchuk, Anatolii M

    2003-01-01

    Methods and results of searching for stellar mass black holes in binary systems and for supermassive black holes in galactic nuclei of different types are described. As of now (June 2002), a total of 100 black hole candidates are known. All the necessary conditions Einstein's General Relativity imposes on the observational properties of black holes are satisfied for candidate objects available, thus further assuring the existence of black holes in the Universe. Prospects for obtaining sufficient criteria for reliably distinguishing candidate black holes from real black holes are discussed. (reviews of topical problems)

  6. Noncommutative black holes

    Energy Technology Data Exchange (ETDEWEB)

    Lopez-DomInguez, J C [Instituto de Fisica de la Universidad de Guanajuato PO Box E-143, 37150 Leoen Gto. (Mexico); Obregon, O [Instituto de Fisica de la Universidad de Guanajuato PO Box E-143, 37150 Leoen Gto. (Mexico); RamIrez, C [Facultad de Ciencias FIsico Matematicas, Universidad Autonoma de Puebla, PO Box 1364, 72000 Puebla (Mexico); Sabido, M [Instituto de Fisica de la Universidad de Guanajuato PO Box E-143, 37150 Leoen Gto. (Mexico)

    2007-11-15

    We study noncommutative black holes, by using a diffeomorphism between the Schwarzschild black hole and the Kantowski-Sachs cosmological model, which is generalized to noncommutative minisuperspace. Through the use of the Feynman-Hibbs procedure we are able to study the thermodynamics of the black hole, in particular, we calculate Hawking's temperature and entropy for the 'noncommutative' Schwarzschild black hole.

  7. Aroma peculiarities of apricot (Armeniaca vulgaris Lam. and cherry-plum (Prunus cerasifera Ehrh. flowers

    Directory of Open Access Journals (Sweden)

    В. М. Горіна

    2013-02-01

    Full Text Available In the component composition of volatile solutions determining fragrance of the flowers in apricot and cherry-plum varieties and Prunus brigantiaca Vill. x Armeniaca vulgaris Lam. hybrids there are 36 highest hydrocarbons and benzaldehyde that prevail. There are fewer amounts of the solutions which scare bees (benzaldehyde in the fragrance of cherry-plum varieties as compared to the flowers of apricot and hybrids. At the same time, the content of tricosane, pentacosane, docosane, heneycosane, eicosane, nonadecan that probably attract bees is higher in the cherry-plum flowers than in the fragrance of apricot and hybrid flowers. The average three years yield of cherry-plum plants (Nikitska Zhovta 10,7 and Salgirskaya Rumjanaya 28,5 t/ ha is higher than for apricot (Recolte de Schatene 0,3; Rodnik 2,9; Ananasniy Tsurupinsky 7,4 t/ha and hybrids (8110 – 5,2; 8098 – 6,4 t/ha that could be explained with better pollination of flowers and better fruit formation. Prevailing components of flower aroma of these plants    and their possible link with yield of the objects in questions have been analyzed.

  8. Black hole critical phenomena without black holes

    Indian Academy of Sciences (India)

    large values of Ф, black holes do form and for small values the scalar field ... on the near side of the ridge ultimately evolve to form black holes while those configu- ... The inset shows a bird's eye view looking down on the saddle point.

  9. Black widow spider

    Science.gov (United States)

    ... medlineplus.gov/ency/article/002858.htm Black widow spider To use the sharing features on this page, please enable JavaScript. The black widow spider (Latrodectus) has a shiny black body with a ...

  10. In Vitro Pollen Viability and Pollen Germination in Cherry Laurel (Prunus laurocerasus L.

    Directory of Open Access Journals (Sweden)

    Melekber Sulusoglu

    2014-01-01

    Full Text Available Pollen quality is important for growers and breeders. This study was carried out to determine in vitro pollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasus L.. Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride and IKI (iodine potassium iodide, were used. Pollen traits of genotypes were studied using an in vitro medium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentrations for germination. In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination. The viability rates were different according to genotypes and tests used. The IKI and TTC staining tests and pollen germination had low correlation (r2 = 0.0614 and r2 = 0.0015, resp.. Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability estimated with TTC staining test was better than that estimated with the IKI staining test. 15% sucrose gave the best germination rates in most of the genotypes. Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

  11. In vitro pollen viability and pollen germination in cherry laurel (Prunus laurocerasus L.).

    Science.gov (United States)

    Sulusoglu, Melekber; Cavusoglu, Aysun

    2014-01-01

    Pollen quality is important for growers and breeders. This study was carried out to determine in vitro pollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasus L.). Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride) and IKI (iodine potassium iodide), were used. Pollen traits of genotypes were studied using an in vitro medium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentrations for germination. In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination. The viability rates were different according to genotypes and tests used. The IKI and TTC staining tests and pollen germination had low correlation (r(2) = 0.0614 and r(2) = 0.0015, resp.). Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability estimated with TTC staining test was better than that estimated with the IKI staining test. 15% sucrose gave the best germination rates in most of the genotypes. Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

  12. A Dancing Black Hole

    Science.gov (United States)

    Shoemaker, Deirdre; Smith, Kenneth; Schnetter, Erik; Fiske, David; Laguna, Pablo; Pullin, Jorge

    2002-04-01

    Recently, stationary black holes have been successfully simulated for up to times of approximately 600-1000M, where M is the mass of the black hole. Considering that the expected burst of gravitational radiation from a binary black hole merger would last approximately 200-500M, black hole codes are approaching the point where simulations of mergers may be feasible. We will present two types of simulations of single black holes obtained with a code based on the Baumgarte-Shapiro-Shibata-Nakamura formulation of the Einstein evolution equations. One type of simulations addresses the stability properties of stationary black hole evolutions. The second type of simulations demonstrates the ability of our code to move a black hole through the computational domain. This is accomplished by shifting the stationary black hole solution to a coordinate system in which the location of the black hole is time dependent.

  13. Predicción científica y prescripción en mejora genética vegetal en cuanto Ciencia Aplicada de Diseño: El caso de la mejora de frutales del género Prunus

    Directory of Open Access Journals (Sweden)

    Pedro Martínez-Gómez

    2017-01-01

    Full Text Available La mejora genética vegetal tiene una doble índole científica: Ciencia Empírica de la Naturaleza y Ciencia Aplicada de Diseño. En este contexto el presente trabajo pretende indagar en la predicción científica como rasgo esencial de esta disciplina en cuanto Ciencia Aplicada de Diseño con especial referencia la mejora de frutales de hueso (del género Prunus. En mejora genética vegetal podemos hablar de tres niveles de conocimiento asociados a la biología molecular (nivel micro, la constitución genética de un individuo (nivel meso y al fenotipo o aspecto global de la nueva variedad (nivel macro, que afectan tanto a los tipos de predicción como a las metodologías a aplicar. La predicción constituye el objetivo principal de la mejora genética como Ciencia de Diseño. Es clave tener un conocimiento del futuro posible para poder hacer un nuevo diseño que estará completo al cabo de algunos años, más de 12 años en el caso de los frutales de hueso que es el objeto de este trabajo. Asimismo, es necesario tener en cuenta en el desarrollo de esta predicción científica aplicada a la mejora de Prunus diversas variables internas (la naturaleza genética del material vegetal de partida, las metodologías disponibles, etc. y externas (la aceptación social, los factores medioambientales, los estreses bióticos y abióticos, etc. a la hora de llevar a cabo esta predicción. El grado de conocimiento de estas variables determinará la calidad de la predicción en el diseño de nuevas variedades de Prunus.

  14. Conspecificity of Hanseniaspora nodinigri and Hanseniaspora vineae: comparison by DNA reassociation.

    Science.gov (United States)

    Smith, M T; Poot, G A

    1985-01-01

    Hanseniaspora nodinigri Lachance 1981, found in black knots (caused by Dibotryon morbosum) of Prunus virginiana, was described as a new species, some time after publication of the extensive study by Meyer et al. (1978) on the systematics of Hanseniaspora Zikes and its imperfect counterpart Kloeckera Janke. Lachance delimited the species from other members of the genus because of absence of growth on glucono-delta-lactone. He also stated that this species, although evidently similar in many ways to H. vineae van der Walt et Tscheuschner and H. osmophila (Niehaus) Phaff et al., has 'diverged from them, possibly in its adaptation to growth in association with black knots'.

  15. Environ: E00442 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00442 Prunus humilis, Prunus japonica seed Crude drug ... Prunus humilis [TAX:434060], Prunus jap...onica [TAX:97319] ... Rosaceae (rose family) Prunus humilis, Prunus japonica mature seed (dried) ...

  16. Astrophysical black holes

    CERN Document Server

    Gorini, Vittorio; Moschella, Ugo; Treves, Aldo; Colpi, Monica

    2016-01-01

    Based on graduate school lectures in contemporary relativity and gravitational physics, this book gives a complete and unified picture of the present status of theoretical and observational properties of astrophysical black holes. The chapters are written by internationally recognized specialists. They cover general theoretical aspects of black hole astrophysics, the theory of accretion and ejection of gas and jets, stellar-sized black holes observed in the Milky Way, the formation and evolution of supermassive black holes in galactic centers and quasars as well as their influence on the dynamics in galactic nuclei. The final chapter addresses analytical relativity of black holes supporting theoretical understanding of the coalescence of black holes as well as being of great relevance in identifying gravitational wave signals. With its introductory chapters the book is aimed at advanced graduate and post-graduate students, but it will also be useful for specialists.

  17. Tradition and Culture Change in the Oklahoma Delaware Big House Community: 1867-1924.

    Science.gov (United States)

    1981-01-01

    sore throat Slippery Elm Ulmus fulva xkwikpi Drink from soaked strips of bark used to relieve chills Wild Black Cherry Prunus scrotina mwmfeli Boiled...Berries used to make dye for baskets Pokeberry Phytolacca americana cAk%.ngwtm Tonic given to chickens made from roots Slippery Elm Ulmus fulva xkwilpi...along drainages, forests of elm , ash, and cottonwood trees. The grasslands extended up the rockier col- luvial slopes where they gave way in places to

  18. Lumpy AdS5× S5 black holes and black belts

    International Nuclear Information System (INIS)

    Dias, Óscar J.C.; Santos, Jorge E.; Way, Benson

    2015-01-01

    Sufficiently small Schwarzschild black holes in global AdS 5 ×S 5 are Gregory-Laflamme unstable. We construct new families of black hole solutions that bifurcate from the onset of this instability and break the full SO(6) symmetry group of the S 5 down to SO(5). These new “lumpy" solutions are labelled by the harmonics ℓ. We find evidence that the ℓ=1 branch never dominates the microcanonical/canonical ensembles and connects through a topology-changing merger to a localised black hole solution with S 8 topology. We argue that these S 8 black holes should become the dominant phase in the microcanonical ensemble for small enough energies, and that the transition to Schwarzschild black holes is first order. Furthermore, we find two branches of solutions with ℓ=2. We expect one of these branches to connect to a solution containing two localised black holes, while the other branch connects to a black hole solution with horizon topology S 4 ×S 4 which we call a “black belt”.

  19. Black holes in binary stars

    NARCIS (Netherlands)

    Wijers, R.A.M.J.

    1996-01-01

    Introduction Distinguishing neutron stars and black holes Optical companions and dynamical masses X-ray signatures of the nature of a compact object Structure and evolution of black-hole binaries High-mass black-hole binaries Low-mass black-hole binaries Low-mass black holes Formation of black holes

  20. Black Interpretation, Black American Literature, and Grey Audiences.

    Science.gov (United States)

    Washington, Earl M.

    1981-01-01

    Defines and illustrates language techniques used by Black authors writing to and for Blacks in the 1960s and 1970s. Suggests how language and theme barriers of such literature might be overcome in a contemporary integrated oral interpretation classroom. (PD)

  1. #BlackBabiesMatter: Analyzing Black Religious Media in Conservative and Progressive Evangelical Communities

    Directory of Open Access Journals (Sweden)

    Monique Moultrie

    2017-11-01

    Full Text Available This article explores how conservative and progressive black Protestants interrogate the theological theme of the sacrality of black life through digital media. The innovations of religious media in black evangelical communities remain an understudied phenomenon in African American religion, making this an apt arena for further discovery. This current intervention into the study of African American Religion examines digital activism through examples of religious media produced by blacks for black audiences. This article begins its interrogation of the sacrality of black life by juxtaposing those who contend that Black Babies Matter as pro-birth-oriented, religiously motivated activists with those religious opponents asserting Black Lives Matter who present an intersectional pro-life approach. The comparison of views relies on womanist cultural analysis as its main methodology to analyze and interpret digital media and explore its ramifications for African American Religion.

  2. Black holes. Chapter 6

    International Nuclear Information System (INIS)

    Penrose, R.

    1980-01-01

    Conditions for the formation of a black hole are considered, and the properties of black holes. The possibility of Cygnus X-1 as a black hole is discussed. Einstein's theory of general relativity in relation to the formation of black holes is discussed. (U.K.)

  3. Black ringoids: spinning balanced black objects in d≥5 dimensions — the codimension-two case

    International Nuclear Information System (INIS)

    Kleihaus, Burkhard; Kunz, Jutta; Radu, Eugen

    2015-01-01

    We propose a general framework for the study of asymptotically flat black objects with k+1 equal magnitude angular momenta in d≥5 spacetime dimensions (with 0≤k≤[((d−5)/2)]). In this approach, the dependence on all angular coordinates but one is factorized, which leads to a codimension-two problem. This framework can describe black holes with spherical horizon topology, the simplest solutions corresponding to a class of Myers-Perry black holes. A different set of solutions describes balanced black objects with S"n"+"1×S"2"k"+"1 horizon topology. The simplest members of this family are the black rings (k=0). The solutions with k>0 are dubbed black ringoids. Based on the nonperturbative numerical results found for several values of (n,k), we propose a general picture for the properties and the phase diagram of these solutions and the associated black holes with spherical horizon topology: n=1 black ringoids repeat the k=0 pattern of black rings and Myers-Perry black holes in 5 dimensions, whereas n>1 black ringoids follow the pattern of higher dimensional black rings associated with ‘pinched’ black holes and Myers-Perry black holes.

  4. Black theology in South Africa – A theology of human dignity and black identity

    Directory of Open Access Journals (Sweden)

    Timothy van Aarde

    2016-08-01

    Full Text Available Black theology in South Africa is still relevant 20 years after the apartheid regime ended. It is a theology that gave to Black South Africans human dignity and a black identity. Black theology in South Africa confronted the imbalances of power and abusive power structures through an affirmation of human dignity and the uniqueness of the identity of black people. The biblical narrative of the Exodus is a definitive narrative in American black theology and liberation theology in overcoming oppression understood as political victimisation. Black theology in South Africa is not primarily about power and economics but also about the rediscovery of human dignity and black identity and to a lesser extent about victimisation. A third generation of black theology in South Africa will gain impetus through a rediscovery of human dignity and identity as its core values instead of a Black American liberation theology of victimisation or a Marxist liberation theology of the eradication of all power or economic imbalances.

  5. Genome-wide analysis of the GRAS gene family in Prunus mume.

    Science.gov (United States)

    Lu, Jiuxing; Wang, Tao; Xu, Zongda; Sun, Lidan; Zhang, Qixiang

    2015-02-01

    Prunus mume is an ornamental flower and fruit tree in Rosaceae. We investigated the GRAS gene family to improve the breeding and cultivation of P. mume and other Rosaceae fruit trees. The GRAS gene family encodes transcriptional regulators that have diverse functions in plant growth and development, such as gibberellin and phytochrome A signal transduction, root radial patterning, and axillary meristem formation and gametogenesis in the P. mume genome. Despite the important roles of these genes in plant growth regulation, no findings on the GRAS genes of P. mume have been reported. In this study, we discerned phylogenetic relationships of P. mume GRAS genes, and their locations, structures in the genome and expression levels of different tissues. Out of 46 identified GRAS genes, 45 were located on the 8 P. mume chromosomes. Phylogenetic results showed that these genes could be classified into 11 groups. We found that Group X was P. mume-specific, and three genes of Group IX clustered with the rice-specific gene Os4. We speculated that these genes existed before the divergence of dicotyledons and monocotyledons and were lost in Arabidopsis. Tissue expression analysis indicated that 13 genes showed high expression levels in roots, stems, leaves, flowers and fruits, and were related to plant growth and development. Functional analysis of 24 GRAS genes and an orthologous relationship analysis indicated that many functioned during plant growth and flower and fruit development. Our bioinformatics analysis provides valuable information to improve the economic, agronomic and ecological benefits of P. mume and other Rosaceae fruit trees.

  6. Black hole hair removal

    International Nuclear Information System (INIS)

    Banerjee, Nabamita; Mandal, Ipsita; Sen, Ashoke

    2009-01-01

    Macroscopic entropy of an extremal black hole is expected to be determined completely by its near horizon geometry. Thus two black holes with identical near horizon geometries should have identical macroscopic entropy, and the expected equality between macroscopic and microscopic entropies will then imply that they have identical degeneracies of microstates. An apparent counterexample is provided by the 4D-5D lift relating BMPV black hole to a four dimensional black hole. The two black holes have identical near horizon geometries but different microscopic spectrum. We suggest that this discrepancy can be accounted for by black hole hair - degrees of freedom living outside the horizon and contributing to the degeneracies. We identify these degrees of freedom for both the four and the five dimensional black holes and show that after their contributions are removed from the microscopic degeneracies of the respective systems, the result for the four and five dimensional black holes match exactly.

  7. A remarkable synergistic effect at the transcriptomic level in peach fruits doubly infected by prunus necrotic ringspot virus and peach latent mosaic viroid.

    Science.gov (United States)

    Herranz, Mari Carmen; Niehl, Annette; Rosales, Marlene; Fiore, Nicola; Zamorano, Alan; Granell, Antonio; Pallas, Vicente

    2013-05-28

    Microarray profiling is a powerful technique to investigate expression changes of large amounts of genes in response to specific environmental conditions. The majority of the studies investigating gene expression changes in virus-infected plants are limited to interactions between a virus and a model host plant, which usually is Arabidopsis thaliana or Nicotiana benthamiana. In the present work, we performed microarray profiling to explore changes in the expression profile of field-grown Prunus persica (peach) originating from Chile upon single and double infection with Prunus necrotic ringspot virus (PNRSV) and Peach latent mosaic viroid (PLMVd), worldwide natural pathogens of peach trees. Upon single PLMVd or PNRSV infection, the number of statistically significant gene expression changes was relatively low. By contrast, doubly-infected fruits presented a high number of differentially regulated genes. Among these, down-regulated genes were prevalent. Functional categorization of the gene expression changes upon double PLMVd and PNRSV infection revealed protein modification and degradation as the functional category with the highest percentage of repressed genes whereas induced genes encoded mainly proteins related to phosphate, C-compound and carbohydrate metabolism and also protein modification. Overrepresentation analysis upon double infection with PLMVd and PNRSV revealed specific functional categories over- and underrepresented among the repressed genes indicating active counter-defense mechanisms of the pathogens during infection. Our results identify a novel synergistic effect of PLMVd and PNRSV on the transcriptome of peach fruits. We demonstrate that mixed infections, which occur frequently in field conditions, result in a more complex transcriptional response than that observed in single infections. Thus, our data demonstrate for the first time that the simultaneous infection of a viroid and a plant virus synergistically affect the host transcriptome in

  8. UH-60M Black Hawk Helicopter (UH-60M Black Hawk)

    Science.gov (United States)

    2016-12-01

    Selected Acquisition Report (SAR) RCS: DD-A&T(Q&A)823-341 UH-60M Black Hawk Helicopter (UH-60M Black Hawk ) As of FY 2017 President’s Budget Defense...Acquisition Management Information Retrieval (DAMIR) March 21, 2016 18:25:45 UNCLASSIFIED UH-60M Black Hawk December 2015 SAR March 21, 2016 18...Operational Requirements Document OSD - Office of the Secretary of Defense O&S - Operating and Support PAUC - Program Acquisition Unit Cost UH-60M Black Hawk

  9. Black American and Nigerian Pentecostalism: A Black Religious ...

    African Journals Online (AJOL)

    Black American and Nigerian Pentecostalism: A Black Religious Schizophrenia, 1910-2010. ... in American and African Pentecostalism as is related to social crisis, the dislocation of masses brought on by economic deprivation, urbanization, the break up of traditional society and consequence loss of traditional values.

  10. Black Tea

    Science.gov (United States)

    ... mental alertness as well as learning, memory, and information processing skills. It is also used for treating headache; ... of carbamazepine. Since black tea contains caffeine, in theory taking black tea with carbamazepine might decrease the ...

  11. Evaluation of apricot (Prunus armeniaca L.) seed kernel as a potential feedstock for the production of liquid bio-fuels and activated carbons

    International Nuclear Information System (INIS)

    Fadhil, Abdelrahman B.

    2017-01-01

    Highlights: • Apricot (Prunus armeniaca L.) is presented as a source for biodiesel, bio-oil and activated carbon. • Methylic and ethylic esters of apricot seed kernel oil conformed to ASTM (D6751) standards. • High yield (43.66% w/w) of bio-oil was produced by pyrolysis of de-oiled seed kernel. • High quality of activated carbon was obtained from the biochar. - Abstract: Production of liquid bio-fuels (biodiesel and bio-oil) as well as activated carbon from one non-edible feedstock, apricot (Prunus armeniaca L.) seed kernel was the main objective of the present research work. The oil was extracted from apricot seed kernel with a yield of 49.44% w/w of kernels. Potassium hydroxide-catalyzed transesterification of apricot (Prunus armeniaca L.) seed kernel oil with methanol and ethanol was then applied to produce methylic and ethylic, respectively. Properties of the obtained biodiesels were evaluated and found conformed to ASTM D 6751 limits. The apricot de-oiled seed kernel was pyrolyzed in a semi-batch reactor for bio-oil production. The effect of the pyrolysis temperatures (350, 400, 450, 500, 550 and 600 °C), pyrolysis time (30, 60, 90, 120 and 150 min) and feed particles size (0.25, 0.40, 0.59 and 0.84 mm) on the bio-oil yield was investigated. The maximum production of bio-oil (43.66% w/w) was achieved at a pyrolysis temperature of 450 °C, 60 min pyrolysis time and a feed particles size of 0.25 mm. The bio-oil obtained under the optimal conditions was characterized by the elemental analysis, FTIR spectroscopy and column chromatography. The FTIR analysis of the produced bio-fuel indicated that it composes mainly of alkanes, alkenes, ketones, carboxylic acids and amines. Properties of the resulting bio-oil were analyzed in terms of calorific value, density, flash point, pH, acid value, pour point and refractive index. The properties were close to those of petroleum fractions and comparable to those of other bio-oils published in literature. Referring to

  12. Black holes are warm

    International Nuclear Information System (INIS)

    Ravndal, F.

    1978-01-01

    Applying Einstein's theory of gravitation to black holes and their interactions with their surroundings leads to the conclusion that the sum of the surface areas of several black holes can never become less. This is shown to be analogous to entropy in thermodynamics, and the term entropy is also thus applied to black holes. Continuing, expressions are found for the temperature of a black hole and its luminosity. Thermal radiation is shown to lead to explosion of the black hole. Numerical examples are discussed involving the temperature, the mass, the luminosity and the lifetime of black mini-holes. It is pointed out that no explosions corresponding to the prediction have been observed. It is also shown that the principle of conservation of leptons and baryons is broken by hot black holes, but that this need not be a problem. The related concept of instantons is cited. It is thought that understanding of thermal radiation from black holes may be important for the development of a quantified gravitation theory. (JIW)

  13. Is It because I'm Black? A Black Female Research Experience

    Science.gov (United States)

    Maylor, Uvanney

    2009-01-01

    This article examines what it means to be a Black female researcher in contemporary Britain. Drawing on Black feminist theory and critical race theory (CRT), this article seeks to highlight some of the experiences and challenges that Black female researchers face when undertaking research, particularly research that has diversity, equality or…

  14. Differentiation of closely related but biologically distinct cherry isolates of Prunus necrotic ringspot virus by polymerase chain reaction.

    Science.gov (United States)

    Hammond, R W; Crosslin, J M; Pasini, R; Howell, W E; Mink, G I

    1999-07-01

    Prunus necrotic ringspot ilarvirus (PNRSV) exists as a number of biologically distinct variants which differ in host specificity, serology, and pathology. Previous nucleotide sequence alignment and phylogenetic analysis of cloned reverse transcription-polymerase chain reaction (RT-PCR) products of several biologically distinct sweet cherry isolates revealed correlations between symptom type and the nucleotide and amino acid sequences of the 3a (putative movement protein) and 3b (coat protein) open reading frames. Based upon this analysis, RT-PCR assays have been developed that can identify isolates displaying different symptoms and serotypes. The incorporation of primers in a multiplex PCR protocol permits rapid detection and discrimination among the strains. The results of PCR amplification using type-specific primers that amplify a portion of the coat protein gene demonstrate that the primer-selection procedure developed for PNRSV constitutes a reliable method of viral strain discrimination in cherry for disease control and will also be useful for examining biological diversity within the PNRSV virus group.

  15. Synthesis of disaccharides using β-glucosidases from Aspergillus niger, A. awamori and Prunus dulcis.

    Science.gov (United States)

    da Silva, Ayla Sant'Ana; Molina, Javier Freddy; Teixeira, Ricardo Sposina Sobral; Valdivieso Gelves, Luis G; Bon, Elba P S; Ferreira-Leitão, Viridiana S

    2017-11-01

    Glucose conversion into disaccharides was performed with β-glucosidases from Prunus dulcis (β-Pd), Aspergillus niger (β-An) and A. awamori (β-Aa), in reactions containing initial glucose of 700 and 900 g l -1 . The reactions' time courses were followed regarding glucose and product concentrations. In all cases, there was a predominant formation of gentiobiose over cellobiose and also of oligosaccharides with a higher molecular mass. For reactions containing 700 g glucose l -1 , the final substrate conversions were 33, 38, and 23.5% for β-An, β-Aa, and β-Pd, respectively. The use of β-An yielded 103 g gentiobiose l -1 (15.5% yield), which is the highest reported for a fungal β-glucosidase. The increase in glucose concentration to 900 g l -1 resulted in a significant increase in disaccharide synthesis by β-Pd, reaching 128 g gentiobiose l -1 (15% yield), while for β-An and β-Aa, there was a shift toward the synthesis of higher oligosaccharides. β-Pd and the fungal β-An and β-Aa β-glucosidases present quite dissimilar kinetics and selective properties regarding the synthesis of disaccharides; while β-Pd showed the highest productivity for gentiobiose synthesis, β-An presented the highest specificity.

  16. Selection of autochthonous sour cherry (Prunus cerasus L. genotypes in Feketić region

    Directory of Open Access Journals (Sweden)

    Radičević Sanja

    2012-01-01

    Full Text Available Autochthonous genotypes of fruit species are very important source of genetic variability and valuable material for breeding work. Fruit Research Institute-Čačak has a long tradition of studying autochthonous genotypes of temperate fruits sporadically spread and preserved in some localities in Serbia. Over 2005-2006, the following properties of nine autochthonous sour cherry genotypes grown in Feketic region were investigated: flowering and ripening time, pomological properties, biochemical composition of fruits and field resistance to causal agents of cherry diseases - cherry leaf spot (Blumeriella jaapii (Rehm. v. Arx., shot-hole (Clasterosporium carpophilum (Lév. Aderh. and brown rot (Monilinia laxa /Ader et Ruhl./ Honey ex Whetz.. The genotypes were tested for the presence of Prune dwarf virus and Prunus necrotic ring spot virus. In majority of genotypes fruits were large, with exceptional organoleptical properties, whereas ripening time was in the first ten or twenty days of June. The highest fruit weight was observed in F-1 genotype (8.1 g. The highest soluble solids and total sugars content were found in F- 4 genotype (17.60% and 14.25%, respectively. As for field resistance to causal agents of diseases and good pomo-technological properties, F-1, F-2, F-3, F-7 and F-8 genotypes were singled out. [Projekat Ministarstva nauke Republike Srbije, br. TR31064

  17. The stable problem of the black-hole connected region in the Schwarzschild black hole

    OpenAIRE

    Tian, Guihua

    2005-01-01

    The stability of the Schwarzschild black hole is studied. Using the Painlev\\'{e} coordinate, our region can be defined as the black-hole-connected region(r>2m, see text) of the Schwarzschild black hole or the white-hole-connected region(r>2m, see text) of the Schwarzschild black hole. We study the stable problems of the black-hole-connected region. The conclusions are: (1) in the black-hole-connected region, the initially regular perturbation fields must have real frequency or complex frequen...

  18. Quantum aspects of black holes

    CERN Document Server

    2015-01-01

    Beginning with an overview of the theory of black holes by the editor, this book presents a collection of ten chapters by leading physicists dealing with the variety of quantum mechanical and quantum gravitational effects pertinent to black holes. The contributions address topics such as Hawking radiation, the thermodynamics of black holes, the information paradox and firewalls, Monsters, primordial black holes, self-gravitating Bose-Einstein condensates, the formation of small black holes in high energetic collisions of particles, minimal length effects in black holes and small black holes at the Large Hadron Collider. Viewed as a whole the collection provides stimulating reading for researchers and graduate students seeking a summary of the quantum features of black holes.

  19. Black holes and beyond

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2002-02-01

    Belief in the existence of black holes is the ultimate act of faith for a physicist. First suggested by the English clergyman John Michell in the year 1784, the gravitational pull of a black hole is so strong that nothing - not even light - can escape. Gravity might be the weakest of the fundamental forces but black-hole physics is not for the faint-hearted. Black holes present obvious problems for would-be observers because they cannot, by definition, be seen with conventional telescopes - although before the end of the decade gravitational-wave detectors should be able to study collisions between black holes. Until then astronomers can only infer the existence of a black hole from its gravitational influence on other matter, or from the X-rays emitted by gas and dust as they are dragged into the black hole. However, once this material passes through the 'event horizon' that surrounds the black hole, we will never see it again - not even with X-ray specs. Despite these observational problems, most physicists and astronomers believe that black holes do exist. Small black holes a few kilometres across are thought to form when stars weighing more than about two solar masses collapse under the weight of their own gravity, while supermassive black holes weighing millions of solar masses appear to be present at the centre of most galaxies. Moreover, some brave physicists have proposed ways to make black holes - or at least event horizons - in the laboratory. The basic idea behind these 'artificial black holes' is not to compress a large amount of mass into a small volume, but to reduce the speed of light in a moving medium to less than the speed of the medium and so create an event horizon. The parallels with real black holes are not exact but the experiments could shed new light on a variety of phenomena. The first challenge, however, is to get money for the research. One year on from a high-profile meeting on artificial black holes in London, for

  20. Complicated Contradictions Amid Black Feminism and Millennial Black Women Teachers Creating Curriculum for Black Girls

    Science.gov (United States)

    Nyachae, Tiffany M.

    2016-01-01

    Millennial Black women teachers wrestle with two simultaneous burdens: disrupting the racist and sexist status quo of schooling through curriculum, and employing tactics to survive school politics among their majority White women colleagues. This article describes how the "Sisters of Promise" (SOP) curriculum aligned with Black feminism…

  1. Rotating black holes at future colliders. III. Determination of black hole evolution

    International Nuclear Information System (INIS)

    Ida, Daisuke; Oda, Kin-ya; Park, Seong Chan

    2006-01-01

    TeV scale gravity scenario predicts that the black hole production dominates over all other interactions above the scale and that the Large Hadron Collider will be a black hole factory. Such higher-dimensional black holes mainly decay into the standard model fields via the Hawking radiation whose spectrum can be computed from the greybody factor. Here we complete the series of our work by showing the greybody factors and the resultant spectra for the brane-localized spinor and vector field emissions for arbitrary frequencies. Combining these results with the previous works, we determine the complete radiation spectra and the subsequent time evolution of the black hole. We find that, for a typical event, well more than half a black hole mass is emitted when the hole is still highly rotating, confirming our previous claim that it is important to take into account the angular momentum of black holes

  2. BlackBerry For Dummies

    CERN Document Server

    Kao, Robert

    2010-01-01

    Get the most juice out of your BlackBerry handheld!. Feature-rich and complex, the BlackBerry is the number one smartphone in the corporate world is among the most popular handhelds for business users. This new and updated edition includes all the latest and greatest information on new and current BlackBerry mobile devices. Covering a range of valuable how-to topics, this helpful guide explores the BlackBerry's most useful features, techniques for getting the most out of your BlackBerry, and practical information about power usage.: Covers all aspects of the number one smartphone in the corpor

  3. Black Hole Area Quantization rule from Black Hole Mass Fluctuations

    OpenAIRE

    Schiffer, Marcelo

    2016-01-01

    We calculate the black hole mass distribution function that follows from the random emission of quanta by Hawking radiation and with this function we calculate the black hole mass fluctuation. From a complete different perspective we regard the black hole as quantum mechanical system with a quantized event horizon area and transition probabilities among the various energy levels and then calculate the mass dispersion. It turns out that there is a perfect agreement between the statistical and ...

  4. Framing black boys: parent, teacher, and student narratives of the academic lives of black boys.

    Science.gov (United States)

    Rowley, Stephanie J; Ross, Latisha; Lozada, Fantasy T; Williams, Amber; Gale, Adrian; Kurtz-Costes, Beth

    2014-01-01

    The discourse on Black boys tends to suggest that Black boys are in complete peril. We begin with evidence that Black boys are excelling in certain contexts (i.e., in certain states, in certain schools, and in certain courses). We then discuss the ways in which the narratives used by parents, teachers, and Black boys themselves may serve to further reinforce views that Black boys are beyond hope. Research on Black parents suggests that they tend to view their sons as vulnerable and have lower expectations for sons than for daughters. Studies of teachers show that they tend to view Black boys as unteachable, as social problems, and as scary. Research on Black boys shows that they are sometimes complicit in supporting these narratives by engaging in negative or ste reotypical behavior. We also include recent research that includes counter-narratives of Black boys. We end with suggestions for future research.

  5. Chemical characterization and thermal properties of kernel oils from Tunisian peach and nectarine varieties of Prunus persica

    International Nuclear Information System (INIS)

    Chamli, D.; Bootello, M.A.; Bouali, I.; Jouhri, S.; Boukhchina, S.; Martínez-Force, S.

    2017-01-01

    A comparative study was conducted to determine the fatty acids, triacylglycerol compositions and thermal properties of Tunisian kernel oils from the Prunus persica varieties, peach and nectarine, grown in two areas of Tunisia, Gabes and Morneg. Qualitatively, the fatty acids composition and triacylglycerol species were identical for all samples. Oleic acid (67.7-75.0%) was the main fatty acid, followed by linoleic (15.7-22.1%) and palmitic (5.6-6.3%) acids. The major triacylglycerol species were triolein, OOO (38.4-50.5%), followed by OOL (18.2-23.2%), POO (8.3-9.7%) and OLL (6.3-10.1%). The thermal profiles were highly influenced by the high content of triolein due to the importance of oleic acid in these oils. Moreover, the fatty acids distribution in TAG external positions was determined as corresponding to an α asymmetry coefficient that was between 0.10 and 0.12, indicating a high asymmetry in the distribution of saturated fatty acids in the position sn-1 and sn-3 in the TAG species of all samples. [es

  6. Chemical characterization and thermal properties of kernel oils from Tunisian peach and nectarine varieties of Prunus persica

    Directory of Open Access Journals (Sweden)

    D. Chamli

    2017-09-01

    Full Text Available A comparative study was conducted to determine the fatty acids, triacylglycerol compositions and thermal properties of Tunisian kernel oils from the Prunus persica varieties, peach and nectarine, grown in two areas of Tunisia, Gabes and Morneg. Qualitatively, the fatty acids composition and triacylglycerol species were identical for all samples. Oleic acid (67.7-75.0% was the main fatty acid, followed by linoleic (15.7-22.1% and palmitic (5.6-6.3% acids. The major triacylglycerol species were triolein, OOO (38.4-50.5%, followed by OOL (18.2-23.2%, POO (8.3-9.7% and OLL (6.3-10.1%. The thermal profiles were highly influenced by the high content of triolein due to the importance of oleic acid in these oils. Moreover, the fatty acids distribution in TAG external positions was determined as corresponding to an α asymmetry coefficient that was between 0.10 and 0.12, indicating a high asymmetry in the distribution of saturated fatty acids in the position sn-1 and sn-3 in the TAG species of all samples.

  7. The Black Family as Educator.

    Science.gov (United States)

    Wallace, Joan

    The black family is the primary socializing agent of the black child and, thus, the primary educator. The culture of blacks in America, in which the child is steeped, is unique, complex and rich-the result of a convergence and fusion of African, American, and European influences. In its education of the black child, the black family must deal,…

  8. Monopole Black Hole Skyrmions

    OpenAIRE

    Moss, Ian G; Shiiki, N; Winstanley, E

    2000-01-01

    Charged black hole solutions with pion hair are discussed. These can be\\ud used to study monopole black hole catalysis of proton decay.\\ud There also exist\\ud multi-black hole skyrmion solutions with BPS monopole behaviour.

  9. MAINTENANCE OF ECOLOGICALLY SIGNIFICANT GENETIC VARIATION IN THE TIGER SWALLOWTAIL BUTTERFLY THROUGH DIFFERENTIAL SELECTION AND GENE FLOW.

    Science.gov (United States)

    Bossart, J L; Scriber, J M

    1995-12-01

    Differential selection in a heterogeneous environment is thought to promote the maintenance of ecologically significant genetic variation. Variation is maintained when selection is counterbalanced by the homogenizing effects of gene flow and random mating. In this study, we examine the relative importance of differential selection and gene flow in maintaining genetic variation in Papilio glaucus. Differential selection on traits contributing to successful use of host plants (oviposition preference and larval performance) was assessed by comparing the responses of southern Ohio, north central Georgia, and southern Florida populations of P. glaucus to three hosts: Liriodendron tulipifera, Magnolia virginiana, and Prunus serotina. Gene flow among populations was estimated using allozyme frequencies from nine polymorphic loci. Significant genetic differentiation was observed among populations for both oviposition preference and larval performance. This differentiation was interpreted to be the result of selection acting on Florida P. glaucus for enhanced use of Magnolia, the prevalent host in Florida. In contrast, no evidence of population differentiation was revealed by allozyme frequencies. F ST -values were very small and Nm, an estimate of the relative strengths of gene flow and genetic drift, was large, indicating that genetic exchange among P. glaucus populations is relatively unrestricted. The contrasting patterns of spatial differentiation for host-use traits and lack of differentiation for electrophoretically detectable variation implies that differential selection among populations will be counterbalanced by gene flow, thereby maintaining genetic variation for host-use traits. © 1995 The Society for the Study of Evolution.

  10. Skyrmion black hole hair: Conservation of baryon number by black holes and observable manifestations

    Energy Technology Data Exchange (ETDEWEB)

    Dvali, Gia [Arnold Sommerfeld Center, Ludwig-Maximilians-Universität, 80333 München (Germany); Max-Planck-Institut für Physik, Werner-Heisenberg-Institut, 80805 München (Germany); Center for Cosmology and Particle Physics, Department of Physics, New York University, 4 Washington Place, New York, NY 10003 (United States); Gußmann, Alexander, E-mail: alexander.gussmann@physik.uni-muenchen.de [Arnold Sommerfeld Center, Ludwig-Maximilians-Universität, 80333 München (Germany)

    2016-12-15

    We show that the existence of black holes with classical skyrmion hair invalidates standard proofs that global charges, such as the baryon number, cannot be conserved by a black hole. By carefully analyzing the standard arguments based on a Gedankenexperiment in which a black hole is seemingly-unable to return the baryon number that it swallowed, we identify inconsistencies in this reasoning, which does not take into the account neither the existence of skyrmion black holes nor the baryon/skyrmion correspondence. We then perform a refined Gedankenexperiment by incorporating the new knowledge and show that no contradiction with conservation of baryon number takes place at any stage of black hole evolution. Our analysis also indicates no conflict between semi-classical black holes and the existence of baryonic gauge interaction arbitrarily-weaker than gravity. Next, we study classical cross sections of a minimally-coupled massless probe scalar field scattered by a skyrmion black hole. We investigate how the skyrmion hair manifests itself by comparing this cross section with the analogous cross section caused by a Schwarzschild black hole which has the same ADM mass as the skyrmion black hole. Here we find an order-one difference in the positions of the characteristic peaks in the cross sections. The peaks are shifted to smaller scattering angles when the skyrmion hair is present. This comes from the fact that the skyrmion hair changes the near horizon geometry of the black hole when compared to a Schwarzschild black hole with same ADM mass. We keep the study of this second aspect general so that the qualitative results which we obtain can also be applied to black holes with classical hair of different kind.

  11. Statistical black-hole thermodynamics

    International Nuclear Information System (INIS)

    Bekenstein, J.D.

    1975-01-01

    Traditional methods from statistical thermodynamics, with appropriate modifications, are used to study several problems in black-hole thermodynamics. Jaynes's maximum-uncertainty method for computing probabilities is used to show that the earlier-formulated generalized second law is respected in statistically averaged form in the process of spontaneous radiation by a Kerr black hole discovered by Hawking, and also in the case of a Schwarzschild hole immersed in a bath of black-body radiation, however cold. The generalized second law is used to motivate a maximum-entropy principle for determining the equilibrium probability distribution for a system containing a black hole. As an application we derive the distribution for the radiation in equilibrium with a Kerr hole (it is found to agree with what would be expected from Hawking's results) and the form of the associated distribution among Kerr black-hole solution states of definite mass. The same results are shown to follow from a statistical interpretation of the concept of black-hole entropy as the natural logarithm of the number of possible interior configurations that are compatible with the given exterior black-hole state. We also formulate a Jaynes-type maximum-uncertainty principle for black holes, and apply it to obtain the probability distribution among Kerr solution states for an isolated radiating Kerr hole

  12. Black holes without firewalls

    Science.gov (United States)

    Larjo, Klaus; Lowe, David A.; Thorlacius, Larus

    2013-05-01

    The postulates of black hole complementarity do not imply a firewall for infalling observers at a black hole horizon. The dynamics of the stretched horizon, that scrambles and reemits information, determines whether infalling observers experience anything out of the ordinary when entering a large black hole. In particular, there is no firewall if the stretched horizon degrees of freedom retain information for a time of the order of the black hole scrambling time.

  13. Fear of a Black femme: The existential conundrum of embodying a Black femme identity while being a professor of Black, queer, and feminist studies.

    Science.gov (United States)

    Story, Kaila Adia

    2017-10-02

    Although a Black femme identity has been defined and embodied by many as an identity with Black feminist roots and revolutionary potentials, Black femmes are still rendered hypervisible and invisible through racist and heteronormative politics. Similarly, embodying a Black femme identity as a professor in academia often engenders these same pretenses of hypervisibility and invisibility. This essay explores what this existential conundrum has been for me as both a Black femme and professor of Black queer and feminist studies, while illuminating the mix of forces within academia that have attempted to stifle my chosen sexual identity and gendered performance.

  14. Plant size and abiotic factors determine the intra-specific variation in the multi-stemmed architecture of Prunus lusitanica in the Northeast limit of its global distribution

    Directory of Open Access Journals (Sweden)

    A. Muñoz Costa

    2013-12-01

    Full Text Available Aim of study: The present work provides novel insights on factors (either intrinsic or extrinsic that trigger sprouting in woody species living at range margins. We aim to explain the inter-individual variability in the multi-stemmed architecture of Prunus lusitanica L., an Iberian evergreen relict tree related to the Tertiary flora.Area of study: Northeastern Mediterranean mountains of the Iberian Peninsula, the Northeast limit of the global distribution of the species.Material and Methods: We gathered data on two modes of vegetative reproduction, basal and layering sprouts, in 288 clumps of Prunus lusitanica from four populations. We modeled and analyzed the effect of environmental factors (topography, canopy cover, soil moisture and disturbances and plant size (diameter at breast height on sprouting by means of Generalized Linear Model and other statistical approaches.Main results: Plant size arises as the principal factor to explain the variability of the numbers of both types of sprouts yet it is not a trigger factor. Natural and anthropogenic disturbances promote basal and layering shoots, while tree canopy is mainly relevant for basal shoots, and slope and soil moisture are significant factors for layering shoots.Research highlights: The multi-stemmed architecture of P. lusitanica at the Northeastern limit of its worldwide distribution is triggered by local environmental factors and disturbances. Each external factor shows different levels of influence on the variability and type of vegetative reproduction yet the intensity of the response is driven by the size of the largest trunk of each clump.Key words: vegetative reproduction; sprouting; disturbances; woody plants; relict tree; subtropical; Iberian Peninsula.

  15. Black Lives Matter of Black Identity Extremist? : The FBI, Black Activists and the Struggle for Victimhood

    NARCIS (Netherlands)

    Mutsaers, Paul

    2017-01-01

    The question 'who is the victim?' is an important social directive that shapes the struggles for victimhood in which Black Lives Matters, U.S. police forces and their various (counter)publics are currently engaging. This column begins with a controversial FBI report on so-called Black Identity

  16. Einstein-Gauss-Bonnet metrics: black holes, black strings and a staticity theorem

    International Nuclear Information System (INIS)

    Bogdanos, C.; Charmousis, C.; Gouteraux, B.; Zegers, R.

    2009-01-01

    We find the general solution of the 6-dimensional Einstein-Gauss-Bonnet equations in a large class of space and time-dependent warped geometries. Several distinct families of solutions are found, some of which include black string metrics, space and time-dependent solutions and black holes with exotic horizons. Among these, some are shown to verify a Birkhoff type staticity theorem, although here, the usual assumption of maximal symmetry on the horizon is relaxed, allowing exotic horizon geometries. We provide explicit examples of such static exotic black holes, including ones whose horizon geometry is that of a Bergman space. We find that the situation is very different from higher-dimensional general relativity, where Einstein spaces are admissible black hole horizons and the associated black hole potential is not even affected. In Einstein-Gauss-Bonnet theory, on the contrary, the non-trivial Weyl tensor of such exotic horizons is exposed to the bulk dynamics through the higher order Gauss-Bonnet term, severely constraining the allowed horizon geometries and adding a novel charge-like parameter to the black hole potential. The latter is related to the Euler characteristic of the four-dimensional horizon and provides, in some cases, additional black hole horizons.

  17. Accreting Black Holes

    OpenAIRE

    Begelman, Mitchell C.

    2014-01-01

    I outline the theory of accretion onto black holes, and its application to observed phenomena such as X-ray binaries, active galactic nuclei, tidal disruption events, and gamma-ray bursts. The dynamics as well as radiative signatures of black hole accretion depend on interactions between the relatively simple black-hole spacetime and complex radiation, plasma and magnetohydrodynamical processes in the surrounding gas. I will show how transient accretion processes could provide clues to these ...

  18. Naked black holes

    International Nuclear Information System (INIS)

    Horowitz, G.T.; Ross, S.F.

    1997-01-01

    It is shown that there are large static black holes for which all curvature invariants are small near the event horizon, yet any object which falls in experiences enormous tidal forces outside the horizon. These black holes are charged and near extremality, and exist in a wide class of theories including string theory. The implications for cosmic censorship and the black hole information puzzle are discussed. copyright 1997 The American Physical Society

  19. In situ seasonal study of the volatile production of almonds (Prunus dulcis) var. 'Nonpareil' and relationship to navel orangeworm.

    Science.gov (United States)

    Beck, John J; Merrill, Glory B; Higbee, Bradley S; Light, Douglas M; Gee, Wai S

    2009-05-13

    Nonpareil almonds, Prunus dulcis , account for the largest percentage of almond varieties grown in the Central Valley of California. Several studies have investigated the various nonvolatile and volatile components of various plant parts; however, the volatile organic compound (VOC) emission of almonds from a single cultivar has not been studied over the course of a growing season. This aspect is particularly relevant to research concerning the navel orangeworm (NOW), a major insect pest of almonds and other tree nuts. Despite the continued presence of NOW, the identification of particular VOCs and their relationship to NOW have not been addressed. The VOC emission of Nonpareil almonds was collected in situ over the course of a growing season by solid-phase microextraction (SPME). The VOCs (Z)-hex-3-enyl acetate, (Z)-hex-3-enyl butyrate, undecan-2-ol, beta-bourbonene, and tetradecane were present for the majority of the days investigated. Several VOCs exhibited positive electroantennographic signals from male and/or female NOW moths.

  20. Whole-plant allocation to storage and defense in juveniles of related evergreen and deciduous shrub species.

    Science.gov (United States)

    Wyka, T P; Karolewski, P; Żytkowiak, R; Chmielarz, P; Oleksyn, J

    2016-05-01

    In evergreen plants, old leaves may contribute photosynthate to initiation of shoot growth in the spring. They might also function as storage sites for carbohydrates and nitrogen (N). We hence hypothesized that whole-plant allocation of carbohydrates and N to storage in stems and roots may be lower in evergreen than in deciduous species. We selected three species pairs consisting of an evergreen and a related deciduous species: Mahonia aquifolium (Pursh) Nutt. and Berberis vulgaris L. (Berberidaceae), Prunus laurocerasus L. and Prunus serotina Ehrh. (Rosaceae), and Viburnum rhytidophyllum Hemsl. and Viburnum lantana L. (Adoxaceae). Seedlings were grown outdoors in pots and harvested on two dates during the growing season for the determination of biomass, carbohydrate and N allocation ratios. Plant size-adjusted pools of nonstructural carbohydrates in stems and roots were lower in the evergreen species of Berberidaceae and Adoxaceae, and the slope of the carbohydrate pool vs plant biomass relationship was lower in the evergreen species of Rosaceae compared with the respective deciduous species, consistent with the leading hypothesis. Pools of N in stems and roots, however, did not vary with leaf habit. In all species, foliage contained more than half of the plant's nonstructural carbohydrate pool and, in late summer, also more than half of the plant's N pool, suggesting that in juvenile individuals of evergreen species, leaves may be a major storage site. Additionally, we hypothesized that concentration of defensive phenolic compounds in leaves should be higher in evergreen than in deciduous species, because the lower carbohydrate pool in stems and roots of the former restricts their capacity for regrowth following herbivory and also because of the need to protect their longer-living foliage. Our results did not support this hypothesis, suggesting that evergreen plants may rely predominantly on structural defenses. In summary, our study indicates that leaf habit has

  1. Legitimizing Blacks in Philosophy

    Directory of Open Access Journals (Sweden)

    Jameliah Shorter-Bourhanou

    2017-12-01

    Full Text Available In its efforts toward improving diversity, the discipline of philosophy has tended to focus on increasing the number of black philosophers. One crucial issue that has received less attention is the extent to which black philosophers are delegitimized in the discipline because their philosophical contributions challenge the status quo. A systematic problem that bars black philosophers from equal and full participation, this delegitimization precludes the emergence of genuine diversity and reveals the importance of interrogating broader attitudes toward black philosophical contributions. In this essay, I argue for radical systematic changes to disciplinary hallmarks of professionalization such as pedagogy, mentoring, publishing, and hiring practices with the aim of legitimizing black philosophers and their contributions.

  2. 3,4-Dihydroxybenzaldehyde Derived from Prunus mume Seed Inhibits Oxidative Stress and Enhances Estradiol Secretion in Human Ovarian Granulosa Tumor Cells

    International Nuclear Information System (INIS)

    Kono, Ryohei; Nomura, Sachiko; Okuno, Yoshiharu; Nakamura, Misa; Maeno, Akihiro; Kagiya, Tomoko; Tokuda, Akihiko; Inada, Ken-ichi; Matsuno, Akira; Utsunomiya, Tomoko; Utsunomiya, Hirotoshi

    2014-01-01

    Granulosa cells form ovarian follicles and play important roles in the growth and maturation of oocytes. The protection of granulosa cells from cellular injury caused by oxidative stress is an effective therapy for female infertility. We here investigated an effective bioactive compound derived from Prunus mume seed extract that protects granulosa cells from hydrogen peroxide (H 2 O 2 )-induced apoptosis. We detected the bioactive compound, 3,4-dihydroxybenzaldehyde (3,4-DHBA), via bioactivity-guided isolation and found that it inhibited the H 2 O 2 -induced apoptosis of granulosa cells. We also showed that 3,4-DHBA promoted estradiol secretion in granulosa cells and enhanced the mRNA expression levels of steroidogenic factor 1, a promoter of key steroidogenic enzymes. These results suggest that P. mume seed extract may have clinical potential for the prevention and treatment of female infertility

  3. Black holes are hot

    International Nuclear Information System (INIS)

    Gibbons, G.

    1976-01-01

    Recent work, which has been investigating the use of the concept of entropy with respect to gravitating systems, black holes and the universe as a whole, is discussed. The resulting theory of black holes assigns a finite temperature to them -about 10 -7 K for ordinary black holes of stellar mass -which is in complete agreement with thermodynamical concepts. It is also shown that black holes must continuously emit particles just like ordinary bodies which have a certain temperature. (U.K.)

  4. Intermediate-Mass Black Holes

    Science.gov (United States)

    Miller, M. Coleman; Colbert, E. J. M.

    2004-01-01

    The mathematical simplicity of black holes, combined with their links to some of the most energetic events in the universe, means that black holes are key objects for fundamental physics and astrophysics. Until recently, it was generally believed that black holes in nature appear in two broad mass ranges: stellar-mass (M~3 20 M⊙), which are produced by the core collapse of massive stars, and supermassive (M~106 1010 M⊙), which are found in the centers of galaxies and are produced by a still uncertain combination of processes. In the last few years, however, evidence has accumulated for an intermediate-mass class of black holes, with M~102 104 M⊙. If such objects exist they have important implications for the dynamics of stellar clusters, the formation of supermassive black holes, and the production and detection of gravitational waves. We review the evidence for intermediate-mass black holes and discuss future observational and theoretical work that will help clarify numerous outstanding questions about these objects.

  5. Investigation on the pollen morphology of traditional cultivars of Prunus species in Sicily

    Directory of Open Access Journals (Sweden)

    Anna Geraci

    2012-08-01

    Full Text Available In this study pollen grains of 13 cultivars and 3 rootstocks belonging to 5 species (P. armeniaca, P. domestica, P. dulcis, P. persica, P. avium of the genus Prunus collected from North-East Sicily were examined for the micromorphological characterization through the scanning electron microscopy (SEM. The length of polar axis (P and the equatorial diameter (E of grain, P/E ratio, the length of colpi (C, diameter of perforations (DP and the number of perforations in 25 μm2 (PN, the width of muri (WM, the distance between muri (DM and their number in 25 μm2 (MN, the width of grooves (WG were measured and their variation was compared among studied taxa. Moreover multivariate statistical analysis was carried out to distinguish morphometric information from measured parameters. All pollen grains are trizonocolpate, isopolar, medium-large sized and their shape varies from prolate to perprolate. Regarding outline pollen grains are subtriangular in polar view and elliptic in equatorial view. Exine sculpturing is striate with perforations on grain surface. The arrangement of ridges appears roughly parallel but too sloped (sometimes curved compared to polar axis, or branched and oriented in different directions, or perfectly parallel or more irregular with bifurcated ridges often sinuous. The analyses showed a great variability (particularly in P. domestica cultivars related in some cases to the diversity in the morphological features of the leaves and the fruits of the investigated entities.

  6. The Effect of Black Peers on Black Test Scores

    Science.gov (United States)

    Armor, David J.; Duck, Stephanie

    2007-01-01

    Recent studies have used increasingly complex methodologies to estimate the effect of peer characteristics--race, poverty, and ability--on student achievement. A paper by Hanushek, Kain, and Rivkin using Texas state testing data has received particularly wide attention because it found a large negative effect of school percent black on black math…

  7. Black hole multiplicity at particle colliders (Do black holes radiate mainly on the brane?)

    International Nuclear Information System (INIS)

    Cavaglia, Marco

    2003-01-01

    If gravity becomes strong at the TeV scale, we may have the chance to produce black holes at particle colliders. In this Letter we revisit some phenomenological signatures of black hole production in TeV-gravity theories. We show that the bulk-to-brane ratio of black hole energy loss during the Hawking evaporation phase depends crucially on the black hole greybody factors and on the particle degrees of freedom. Since the greybody factors have not yet been calculated in the literature, and the particle content at trans-Planckian energies is not known, it is premature to claim that the black hole emits mainly on the brane. We also revisit the decay time and the multiplicity of the decay products of black hole evaporation. We give general formulae for black hole decay time and multiplicity. We find that the number of particles produced during the evaporation phase may be significantly lower than the average multiplicity which has been used in the past literature

  8. Understanding the Black Aesthetic Experience.

    Science.gov (United States)

    Curtis, Marvin V.

    1988-01-01

    Discussing the importance of the Black aesthetic experience, Curtis examines Black cultural heritage and participatory style, the spiritual, and the creation and recreation of Black music. Advocating multicultural music education in teacher training, he suggests that Black music be studied for its value and contribution to society. Lists five ways…

  9. Black Writers' Views of America.

    Science.gov (United States)

    Hairston, Loyle

    1979-01-01

    This article argues that the stagnation, pessimism, and self-pity evident in recent Black writing results in part from the alienation of Black writers from the mainstream of Black life, and in part from the illusions that they share with other Blacks who have embraced the American value system. (Author/EB)

  10. String-Corrected Black Holes

    Energy Technology Data Exchange (ETDEWEB)

    Hubeny, V.

    2005-01-12

    We investigate the geometry of four dimensional black hole solutions in the presence of stringy higher curvature corrections to the low energy effective action. For certain supersymmetric two charge black holes these corrections drastically alter the causal structure of the solution, converting seemingly pathological null singularities into timelike singularities hidden behind a finite area horizon. We establish, analytically and numerically, that the string-corrected two-charge black hole metric has the same Penrose diagram as the extremal four-charge black hole. The higher derivative terms lead to another dramatic effect--the gravitational force exerted by a black hole on an inertial observer is no longer purely attractive. The magnitude of this effect is related to the size of the compactification manifold.

  11. Prunus necrotic ringspot virus Early Invasion and Its Effects on Apricot Pollen Grain Performance.

    Science.gov (United States)

    Amari, Khalid; Burgos, Lorenzo; Pallas, Vicente; Sanchez-Pina, María Amelia

    2007-08-01

    ABSTRACT The route of infection and the pattern of distribution of Prunus necrotic ringspot virus (PNRSV) in apricot pollen were studied. PNRSV was detected both within and on the surface of infected pollen grains. The virus invaded pollen during its early developmental stages, being detected in pollen mother cells. It was distributed uniformly within the cytoplasm of uni- and bicellular pollen grains and infected the generative cell. In mature pollen grains, characterized by their triangular shape, the virus was located mainly at the apertures, suggesting that PNRSV distribution follows the same pattern as the cellular components required for pollen tube germination and cell wall tube synthesis. PNRSV also was localized inside pollen tubes, especially in the growth zone. In vitro experiments demonstrated that infection with PNRSV decreases the germination percentage of pollen grains by more than half and delays the growth of pollen tubes by approximately 24 h. However, although PNRSV infection affected apricot pollen grain performance during germination, the presence of the virus did not completely prevent fertilization, because the infected apricot pollen tubes, once germinated, were able to reach the apricot embryo sacs, which, in the climatic conditions of southeastern Spain, mature later than in other climates. Thus, infected pollen still could play an important role in the vertical transmission of PNRSV in apricot.

  12. Propagation of the endangered Azorean cherry Prunus azorica using stem cuttings and air layering

    Directory of Open Access Journals (Sweden)

    MOREIRA, O.

    2009-01-01

    Full Text Available Prunus azorica (Hort. ex Mouillef. Rivas Mart., Lousã, Fern. Prieto, E. Dias, J.C. Costa &C. Aguiar is an endangered tree endemic to the Azores, with an ecological and ornamentalinterest. The objective of this study was to determine the conditions necessary for thesuccessful propagation of P. azorica by stem cuttings and air-layering. Stem cuttingscollected in March with two apical leaf pairs pruned to 1/3 of their leaf area were submittedto different treatments, including a basal split wound, two rooting mixtures, namely, perlite and peat (1:1 or perlite and natural soil (1:1, and dipping of the base in indole-3-butyric acid (IBA solution at four concentrations (0, 2500, 5000 or 7000 mg/L. After eight weeks 75% rooting was achieved with 75 to 88% survival, without addition of IBA and with split wound using both substrate mixtures. Air layering was conducted in June in branches of adult trees with the addition of 0, 2500 or 3000 mg/L of IBA. After 12 months 100%rooting and survival was recorded in all treatments. Our study thus indicates that P. azorica is a taxon amenable for vegetative propagation by stem cuttings and air layering withoutrequiring addition of IBA to induce rooting. Both methods should be used in order to recover natural populations, when seeds are not available or are available in reduced amounts.

  13. BlackMax: A black-hole event generator with rotation, recoil, split branes, and brane tension

    International Nuclear Information System (INIS)

    Dai Dechang; Starkman, Glenn; Stojkovic, Dejan; Issever, Cigdem; Tseng, Jeff; Rizvi, Eram

    2008-01-01

    We present a comprehensive black-hole event generator, BlackMax, which simulates the experimental signatures of microscopic and Planckian black-hole production and evolution at the LHC in the context of brane world models with low-scale quantum gravity. The generator is based on phenomenologically realistic models free of serious problems that plague low-scale gravity, thus offering more realistic predictions for hadron-hadron colliders. The generator includes all of the black-hole gray-body factors known to date and incorporates the effects of black-hole rotation, splitting between the fermions, nonzero brane tension, and black-hole recoil due to Hawking radiation (although not all simultaneously). The generator can be interfaced with Herwig and Pythia. The main code can be downloaded from http://www-pnp.physics.ox.ac.uk/~issever/BlackMax/blackmax.html.

  14. Internal structure of black holes

    International Nuclear Information System (INIS)

    Cvetic, Mirjam

    2013-01-01

    Full text: We review recent progress that sheds light on the internal structure of general black holes. We first summarize properties of general multi-charged rotating black holes both in four and five dimensions. We show that the asymptotic boundary conditions of these general asymptotically flat black holes can be modified such that a conformal symmetry emerges. These subtracted geometries preserve the thermodynamic properties of the original black holes and are of the Lifshitz type, thus describing 'a black hole in the asymptotically conical box'. Recent efforts employ solution generating techniques to construct interpolating geometries between the original black hole and their subtracted geometries. Upon lift to one dimension higher, these geometries lift to AdS 3 times a sphere, and thus provide a microscopic interpretation of the black hole entropy in terms of dual two-dimensional conformal field theory. (author)

  15. Black supernovae and black holes in non-local gravity

    Energy Technology Data Exchange (ETDEWEB)

    Bambi, Cosimo [Center for Field Theory and Particle Physics and Department of Physics, Fudan University,200433 Shanghai (China); Theoretical Astrophysics, Eberhard-Karls Universität Tübingen,72076 Tübingen (Germany); Malafarina, Daniele [Department of Physics, Nazarbayev University,010000 Astana (Kazakhstan); Modesto, Leonardo [Center for Field Theory and Particle Physics and Department of Physics, Fudan University,200433 Shanghai (China)

    2016-04-22

    In a previous paper, we studied the interior solution of a collapsing body in a non-local theory of gravity super-renormalizable at the quantum level. We found that the classical singularity is replaced by a bounce, after which the body starts expanding. A black hole, strictly speaking, never forms. The gravitational collapse does not create an event horizon but only an apparent one for a finite time. In this paper, we solve the equations of motion assuming that the exterior solution is static. With such an assumption, we are able to reconstruct the solution in the whole spacetime, namely in both the exterior and interior regions. Now the gravitational collapse creates an event horizon in a finite comoving time, but the central singularity is approached in an infinite time. We argue that these black holes should be unstable, providing a link between the scenarios with and without black holes. Indeed, we find a non catastrophic ghost-instability of the metric in the exterior region. Interestingly, under certain conditions, the lifetime of our black holes exactly scales as the Hawking evaporation time.

  16. Black Sea aerosols

    International Nuclear Information System (INIS)

    Hacisalihoglu, G.; Eliyakut, F.; Anwari, M.A.; Ataman, O.Y.; Balkas, T.I.; Tuncel, G.; Olmez, I.

    1991-01-01

    Shipboard, high volume air particulate samples were collected from the Black Sea atmosphere and analyzed by instrumental neutron activation analysis, atomic absorption spectrophotometry and ion chromatography for about 40 elements and ions. Concentrations of elements in the eastern and western parts of the Black Sea are different at the 95% confidence level, with lower concentrations in the eastern Black Sea. Back-trajectories and concentrations of elements in trajectory groups show that Europe accounts for more than 70% of the anthropogenic elements in the atmosphere. The average sulfate concentration was 7 μg/m 3 , which is comparable with rural sulfate levels in western Europe. Fluxes of elements from the atmosphere to the Black Sea are in good agreement with the results of similar flux calculations for other regions

  17. Controle da infestação natural de ceratitis capitata (Wied., 1824 (Diptera, Tephritidae em pêssegos(Prunus persica através das radiações gama Control of naturally infested peaches (Prunus persica by mediterranean fruit fly (Ceratitis capitata through the use of gamma radiation

    Directory of Open Access Journals (Sweden)

    V. Arthur

    1993-12-01

    Full Text Available Determinou-se a dose desinfestante de radiações gama para pêssegos, Prunus persica, infestados com larvas da mosca do Mediterrâneo, Ceratitis capitata. Utilizaram-se frutas de procedência conhecida no campo fazendo-se uma amostragem prévia, constatando-se que cada fruta continha em média nove larvas do último ínstar da mosca praga. As frutas foram irradiadas em uma fonte de Cobalto-60 com as seguintes doses de radiação gama: 0 (test., 25, 50, 100, 200, 400, 600, 800, 1000 e 1200 Gy, sob uma taxa de 58 Gy por minuto. Após a irradiação as frutas foram colocadas em câmaras climatizadas com a temperatura variando entre 23 e 27°C e a umidade relativa variando entre 65 e 75%. Aguardou-se que as larvas deixassem as frutas e se transformassem em pupas e adultos. A dose letal para larvas, pelos resultados obtidos no experimento, concluiu-se ser de 600 Gy. A dose letal para pupas provenientes de larvas irradiadas dentro das frutas foi de 50 Gy, impedindo totalmente a emergência de adultos.Determination of the dose of gamma radiation to disinfest peaches, Prunus pérsica infested with larvae of Ceratitis capitata (Wied., 1824 was made. Fruits were collected in the field, each one holding about nine larvae of the last instar of the fruit-fly. The fruits were irradiated with Cobalt-60 gamma radiation source at the following doses: 0 (control, 25, 50, 100, 200, 400, 600, 800, 1000 and 1200 Gy; at a dose rate of 58 Gy per minute. After irradiation the fruits were kept in a climatic chamber with the temperature adjusted between 23 and 27°C, and relative humidity between 65 and 75 percent, until the larvae left the fruits and were transformed into pupae and adults. It was concluded that the lethal dose of gamma radiation for larvae at the last instar, in naturally infested peaches, was 600 Gy and the dose of 50 Gy inhibited completely the emergency of adults.

  18. New Directions for Black Women?

    Science.gov (United States)

    McGuigan, Dorothy, Ed.

    1978-01-01

    This collection of papers deals with various aspects of the black female experience in America. "The Black Woman in Transition" discusses the effects of sexism and racism on black women with particular reference to employment and education; it is noted that black women, in comparison with other groups in society, suffer a proportionately higher…

  19. Black-spot poison ivy.

    Science.gov (United States)

    Schram, Sarah E; Willey, Andrea; Lee, Peter K; Bohjanen, Kimberly A; Warshaw, Erin M

    2008-01-01

    In black-spot poison ivy dermatitis, a black lacquerlike substance forms on the skin when poison ivy resin is exposed to air. Although the Toxicodendron group of plants is estimated to be the most common cause of allergic contact dermatitis in the United States, black-spot poison ivy dermatitis is relatively rare.

  20. What is black hole?

    Indian Academy of Sciences (India)

    First page Back Continue Last page Overview Graphics. What is black hole? Possible end phase of a star: A star is a massive, luminous ball of plasma having continuous nuclear burning. Star exhausts nuclear fuel →. White Dwarf, Neutron Star, Black Hole. Black hole's gravitational field is so powerful that even ...

  1. Genocide and Black Ecology

    Science.gov (United States)

    Sinnette, Calvin H.

    1972-01-01

    Contends that the survival of black people is in serious jeopardy as is evidenced in contemporary discussions on the worldwide plight of black people, and that an exhaustive study of the problem in its many dimensions is seriously lacking; the moral and ethical issues of genocide require examination from a black perspective. (JW)

  2. Quantum effects in black holes

    International Nuclear Information System (INIS)

    Frolov, V.P.

    1979-01-01

    A strict definition of black holes is presented and some properties with regard to their mass are enumerated. The Hawking quantum effect - the effect of vacuum instability in the black hole gravitational field, as a result of shich the black hole radiates as a heated body is analyzed. It is shown that in order to obtain results on the black hole radiation it is sufficient to predetermine the in-vacuum state at a time moment in the past, when the collapsing body has a large size, and its gravitational field can be neglected. The causes and the place of particle production by the black hole, and also the space-time inside the black hole, are considered

  3. Reconceptualizing successful aging among black women and the relevance of the strong black woman archetype.

    Science.gov (United States)

    Baker, Tamara A; Buchanan, NiCole T; Mingo, Chivon A; Roker, Rosalyn; Brown, Candace S

    2015-02-01

    Although there are multiple pathways to successful aging, little is known of what it means to age successfully among black women. There is a growing body of literature suggesting that black women experience a number of social challenges (sexism and racism) that may present as barriers to aging successfully. Applying aspects of the Strong Black Women ideal, into theoretical concepts of successful aging, may be particularly relevant in understanding which factors impair or promote the ability of black women to age successfully. The Strong Black Women archetype is a culturally salient ideal prescribing that black women render a guise of self-reliance, selflessness, and psychological, emotional, and physical strength. Although this ideal has received considerable attention in the behavioral sciences, it has been largely absent within the gerontology field. Nevertheless, understanding the dynamics of this cultural ideal may enhance our knowledge while developing an appreciation of the black woman's ability to age successfully. Rather than summarize the social, physical, and mental health literature focusing on health outcomes of black women, this conceptual review examines the Strong Black Women archetype and its application to the lived experiences of black women and contributions to current theories of successful aging. Focusing on successful aging exclusively among black women enhances our understanding of this group by considering their identity as women of color while recognizing factors that dictate their ability to age successfully. © The Author 2014. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  4. The molecular variability analysis of the RNA 3 of fifteen isolates of Prunus necrotic ringspot virus sheds light on the minimal requirements for the synthesis of its subgenomic RNA.

    Science.gov (United States)

    Aparicio, Frederic; Pallás, Vicente

    2002-01-01

    The nucleotide sequences of the RNA 3 of fifteen isolates of Prunus necrotic ringspot virus (PNRSV) varying in the symptomatology they cause in six different Prunus spp. were determined. Analysis of the molecular variability has allowed, in addition to study the phylogenetic relationships among them, to evaluate the minimal requirements for the synthesis of the subgenomic RNA in Ilarvirus genus and their comparison to other members of the Bromoviridae family. Computer assisted comparisons led recently to Jaspars (Virus Genes 17, 233-242, 1998) to propose that a hairpin structure in viral minus strand RNA is required for subgenomic promoter activity of viruses from at least two, and possibly all five, genera in the family of Bromoviridae. For PNRSV and Apple mosaic virus two stable hairpins were proposed whereas for the rest of Ilarviruses and the other four genera of the Bromoviridae family only one stable hairpin was predicted. Comparative analysis of this region among the fifteen PNRSV isolates characterized in this study revealed that two of them showed a 12-nt deletion that led to the disappearance of the most proximal hairpin to the initiation site. Interestingly, the only hairpin found in these two isolates is very similar in primary and secondary structure to the one previously shown in Brome mosaic virus to be required for the synthesis of the subgenomic RNA. In this hairpin, the molecular diversity was concentrated mostly at the loop whereas compensatory mutations were observed at the base of the stem strongly suggesting its functional relevance. The evolutionary implications of these observations are discussed.

  5. Carbon black vs. black carbon and other airborne materials containing elemental carbon: Physical and chemical distinctions

    International Nuclear Information System (INIS)

    Long, Christopher M.; Nascarella, Marc A.; Valberg, Peter A.

    2013-01-01

    Airborne particles containing elemental carbon (EC) are currently at the forefront of scientific and regulatory scrutiny, including black carbon, carbon black, and engineered carbon-based nanomaterials, e.g., carbon nanotubes, fullerenes, and graphene. Scientists and regulators sometimes group these EC-containing particles together, for example, interchangeably using the terms carbon black and black carbon despite one being a manufactured product with well-controlled properties and the other being an undesired, incomplete-combustion byproduct with diverse properties. In this critical review, we synthesize information on the contrasting properties of EC-containing particles in order to highlight significant differences that can affect hazard potential. We demonstrate why carbon black should not be considered a model particle representative of either combustion soots or engineered carbon-based nanomaterials. Overall, scientific studies need to distinguish these highly different EC-containing particles with care and precision so as to forestall unwarranted extrapolation of properties, hazard potential, and study conclusions from one material to another. -- Highlights: •Major classes of elemental carbon-containing particles have distinct properties. •Despite similar names, carbon black should not be confused with black carbon. •Carbon black is distinguished by a high EC content and well-controlled properties. •Black carbon particles are characterized by their heterogenous properties. •Carbon black is not a model particle representative of engineered nanomaterials. -- This review demonstrates the significant physical and chemical distinctions between elemental carbon-containing particles e.g., carbon black, black carbon, and engineered nanomaterials

  6. Prunus mume leaf extract lowers blood glucose level in diabetic mice.

    Science.gov (United States)

    Lee, Min Woo; Kwon, Jung Eun; Lee, Young-Jong; Jeong, Yong Joon; Kim, Inhye; Cho, Young Mi; Kim, Yong-Min; Kang, Se Chan

    2016-10-01

    Context Diabetes is a common metabolic disease with long-term complications. Prunus mume Sieb. et Zucc. (Rosaceae) fruits have shown to ameliorate glucose intolerance. However, the antidiabetic effects of P. mume leaves have not been investigated. Objective This study evaluated the effects of P. mume leaf 70% ethanol extract (PMLE) on alleviating diabetes in vivo and in vitro. Materials and methods PMLE was fractionated into n-hexane, dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (BuOH) and water. Polyphenol and flavonoid contents in PMLE fractions were determined using Folin-Ciocalteu reagent and the aluminium chloride colorimetric method, respectively. We evaluated α-glucosidase inhibition using a microplate reader at 400 nm. Adipocyte differentiation by lipid accumulation was measured using Nile Red staining. Male imprinting control region (ICR) mice were injected with streptozotocin (STZ, 100 mg/kg, i.p.). High-fat diets were provided for three weeks prior to PMLE treatments to induce type 2 diabetes. PMLE (0, 5, 25 or 50 mg/kg) was administrated for four weeks with high-fat diets. Results The EtOAc fraction of PMLE inhibited α-glucosidase activity (IC50 = 68.2 μg/mL) and contained 883.5 ± 14.9 mg/g of polyphenols and 820.1 ± 7.7 mg/g of flavonoids. The 50 mg/kg PMLE supplement reduced 40% of blood glucose level compared to obese/diabetes mice. Obese/diabetic mice treated with 50 mg/kg PMLE showed a lower level of triacylglycerol (320.7 ± 20.73 mg/dL) compared to obese/diabetes mice (494.9 ± 14.80 mg/dL). Conclusion The data demonstrate that P. mume leaves exert antidiabetic effects that may be attributable to high concentrations of polyphenols and flavonoids.

  7. Towards further understanding on the antioxidative activities of Prunus persica fruit: A comparative study with four different fractions

    Science.gov (United States)

    Dhingra, Naveen; Sharma, Rajesh; Kar, Anand

    2014-11-01

    In the present study we have evaluated the antioxidant activities of different fractions (hexane, ethyl acetate, n-butanol and aqueous fractions) of Prunus persica fruit. For extraction simple warring blender method was employed and total phenolic and flavonoid contents were correlated with different antioxidant activities (total antioxidant, 2,2-diphenyl-1-picrylhydrazyl (DPPH), H2O2 scavenging, superoxide radical scavenging, iron chelating and their reducing power properties). Different in vitro antioxidant studies showed that ethyl acetate and n-butanol fractions had the maximum activities that were well correlated with total phenolic and flavonoid contents. Maximum yield (25.14 ± 2.2%) was obtained in its aqueous fraction. Both ethyl acetate and n-butanol fractions showed significant inhibitory effects on different antioxidant activities. A significantly high correlation coefficient existed between total antioxidant activities and with total phenolic as well as total flavonoid contents. It appears that ethyl acetate and n-butanol fractions of P. persica may serve as new potential sources of natural antioxidants and could be of therapeutic use in treating several diseases.

  8. Examining African self-consciousness and Black racial identity as predictors of Black men's psychological well-being.

    Science.gov (United States)

    Pierre, Martin R; Mahalik, James R

    2005-02-01

    This study investigated African self-consciousness and Black racial identity as predictors of psychological distress and self-esteem for Black men. One hundred thirty Black men from a college and community sample completed the African Self-Consciousness Scale, the Racial Identity Attitude Scale-B, the Symptom Checklist 90-Revised, and the Coopersmith Self-Esteem Inventory. Canonical correlation analysis found 2 significant roots with the 1st root indicating that Black men whose attitudes reflected Preencounter and Immersion racial identity attitudes and who do not resist against anti-African/Black forces reported greater psychological distress and less esteem. Results from the 2nd root suggested that Black men whose attitudes reflect greater Internalization racial identity attitudes, greater resistance to anti-African/Black forces, and less identification with Blacks reported greater self-esteem. ((c) 2005 APA, all rights reserved).

  9. The angry black woman: the impact of pejorative stereotypes on psychotherapy with black women.

    Science.gov (United States)

    Ashley, Wendy

    2014-01-01

    In the aftermath of slavery and the resulting social, economic, and political effects, Black women have become the victims of negative stereotyping in mainstream American culture. Such stereotypes include the myth of the angry Black woman that characterizes these women as aggressive, ill tempered, illogical, overbearing, hostile, and ignorant without provocation. Symptoms presented by Black women during mental health treatment may reinforce this myth. However, many of the negative characteristics of the angry Black woman developed in response to external stressors and historical factors. Black women also have a unique experience with and expressions of anger that shape the presenting symptoms interpreted by the mental health clinician. This myth and corresponding negative stereotypes significantly affect Black women intrapsychically, interpersonally, and are likely to influence the efficacy of mental health treatment. Understanding and treatment of Black women in a mental health context should be influenced by the cultural norms and sociopolitical dynamics affecting these clients. Successful mental health treatment requires cultural competence and clinicians who are well prepared to navigate the inherent complexities of culture with clients. Awareness of the angry Black woman mythology, including its genesis, manifestations, and the unique experiences of Black women, may raise the standards of cultural competence for clinicians and provide more successful treatment outcomes in working with this population. A case example illustrates the assiduity essential to practicing in a culturally competent manner. A client is presented from a traditional psychotherapeutic perspective and then viewed through a lens that integrates psychotherapeutic practice with conscious awareness of the mythology and stereotypes impacting Black women. Implications for culturally relevant practice are discussed.

  10. Over spinning a black hole?

    Energy Technology Data Exchange (ETDEWEB)

    Bouhmadi-Lopez, Mariam; Cardoso, Vitor; Nerozzi, Andrea; Rocha, Jorge V, E-mail: mariam.bouhmadi@ist.utl.pt, E-mail: vitor.cardoso@ist.utl.pt, E-mail: andrea.nerozzi@ist.utl.pt, E-mail: jorge.v.rocha@ist.utl.pt [CENTRA, Department de Fisica, Instituto Superior Tecnico, Av. Rovisco Pais 1, 1049 Lisboa (Portugal)

    2011-09-22

    A possible process to destroy a black hole consists on throwing point particles with sufficiently large angular momentum into the black hole. In the case of Kerr black holes, it was shown by Wald that particles with dangerously large angular momentum are simply not captured by the hole, and thus the event horizon is not destroyed. Here we reconsider this gedanken experiment for black holes in higher dimensions. We show that this particular way of destroying a black hole does not succeed and that Cosmic Censorship is preserved.

  11. Black Holes in Higher Dimensions

    Directory of Open Access Journals (Sweden)

    Reall Harvey S.

    2008-09-01

    Full Text Available We review black-hole solutions of higher-dimensional vacuum gravity and higher-dimensional supergravity theories. The discussion of vacuum gravity is pedagogical, with detailed reviews of Myers–Perry solutions, black rings, and solution-generating techniques. We discuss black-hole solutions of maximal supergravity theories, including black holes in anti-de Sitter space. General results and open problems are discussed throughout.

  12. Black hole decay as geodesic motion

    International Nuclear Information System (INIS)

    Gupta, Kumar S.; Sen, Siddhartha

    2003-01-01

    We show that a formalism for analyzing the near-horizon conformal symmetry of Schwarzschild black holes using a scalar field probe is capable of describing black hole decay. The equation governing black hole decay can be identified as the geodesic equation in the space of black hole masses. This provides a novel geometric interpretation for the decay of black holes. Moreover, this approach predicts a precise correction term to the usual expression for the decay rate of black holes

  13. The Black Atlantic as reversal: A reappraisal of African and black theology

    Directory of Open Access Journals (Sweden)

    Hans Engdahl

    2017-09-01

    Full Text Available In this article, I will try to do three things. Firstly, pay attention to the notion of Black Atlantic as coined by Paul Gilroy, which in effect could signify a reversal of colonialism and slavery. Secondly, revisit the 1970s and the debate about the relevance of Black theology vis-à-vis African theology, using John Mbiti’s article ‘An African Views American Black Theology’ as entry point. Here, I will discuss contributions also made by Desmond Tutu and James Cone. Thirdly, starting with the premise that both theologies are relevant and soul mates today, which would (probably be confirmed by all the above mentioned at this point in time, an assessment of current voices will be made, that is, Tinyiko Maluleke and Vuyani Vellem on black and African ecclesiologies from a South African perspective, and Lawrence Burnley on the fate of the Black Church in the United States.

  14. Black report up-date

    International Nuclear Information System (INIS)

    1988-12-01

    In 1984, the Black Report published its results on the incidence of cancer and leukaemia in under 25-year olds in the village of Seascale and the surrounding area near the Sellafield nuclear reprocessing plant. Since then the pressure group core, as a result of their own independent research, has discovered ten additional cases of childhood cancer which should have been included in the Black Report and a Further two cases since the Black Report was written. This represents an additional 31% over the initial numbers considered and a review of the Black Report is sought. (UK)

  15. Lifshitz topological black holes

    International Nuclear Information System (INIS)

    Mann, R.B.

    2009-01-01

    I find a class of black hole solutions to a (3+1) dimensional theory gravity coupled to abelian gauge fields with negative cosmological constant that has been proposed as the dual theory to a Lifshitz theory describing critical phenomena in (2+1) dimensions. These black holes are all asymptotic to a Lifshitz fixed point geometry and depend on a single parameter that determines both their area (or size) and their charge. Most of the solutions are obtained numerically, but an exact solution is also obtained for a particular value of this parameter. The thermodynamic behaviour of large black holes is almost the same regardless of genus, but differs considerably for small black holes. Screening behaviour is exhibited in the dual theory for any genus, but the critical length at which it sets in is genus-dependent for small black holes.

  16. Acceleration of black hole universe

    Science.gov (United States)

    Zhang, T. X.; Frederick, C.

    2014-01-01

    Recently, Zhang slightly modified the standard big bang theory and developed a new cosmological model called black hole universe, which is consistent with Mach's principle, governed by Einstein's general theory of relativity, and able to explain all observations of the universe. Previous studies accounted for the origin, structure, evolution, expansion, and cosmic microwave background radiation of the black hole universe, which grew from a star-like black hole with several solar masses through a supermassive black hole with billions of solar masses to the present state with hundred billion-trillions of solar masses by accreting ambient matter and merging with other black holes. This paper investigates acceleration of the black hole universe and provides an alternative explanation for the redshift and luminosity distance measurements of type Ia supernovae. The results indicate that the black hole universe accelerates its expansion when it accretes the ambient matter in an increasing rate. In other words, i.e., when the second-order derivative of the mass of the black hole universe with respect to the time is positive . For a constant deceleration parameter , we can perfectly explain the type Ia supernova measurements with the reduced chi-square to be very close to unity, χ red˜1.0012. The expansion and acceleration of black hole universe are driven by external energy.

  17. Why Blue-Collar Blacks Help Less

    OpenAIRE

    Smith, Sandra Susan; Young, Kara Alexis

    2013-01-01

    Why are blue-collar blacks less likely to help jobseekers than jobholders from other ethnoracial groups or even than more affluent blacks? Drawing from in-depth, semi-structured interviews with 97 black and Latino workers at one large, public sector employer, we find that blue-collar black workers both helped less proactively and rejected more requests for assistance than did blue-collar Latino and white-collar black workers. We attribute blue-collar blacks’ more passive engagement to their...

  18. In vitro and in vivo mapping of the Prunus necrotic ringspot virus coat protein C-terminal dimerization domain by bimolecular fluorescence complementation.

    Science.gov (United States)

    Aparicio, Frederic; Sánchez-Navarro, Jesús A; Pallás, Vicente

    2006-06-01

    Interactions between viral proteins are critical for virus viability. Bimolecular fluorescent complementation (BiFC) technique determines protein interactions in real-time under almost normal physiological conditions. The coat protein (CP) of Prunus necrotic ringspot virus is required for multiple functions in its replication cycle. In this study, the region involved in CP dimerization has been mapped by BiFC in both bacteria and plant tissue. Full-length and C-terminal deleted forms of the CP gene were fused in-frame to the N- and C-terminal fragments of the yellow fluorescent protein. The BiFC analysis showed that a domain located between residues 9 and 27 from the C-end plays a critical role in dimerization. The importance of this C-terminal region in dimer formation and the applicability of the BiFC technique to analyse viral protein interactions are discussed.

  19. Curvature invariant characterization of event horizons of four-dimensional black holes conformal to stationary black holes

    Science.gov (United States)

    McNutt, David D.

    2017-11-01

    We introduce three approaches to generate curvature invariants that transform covariantly under a conformal transformation of a four-dimensional spacetime. For any black hole conformally related to a stationary black hole, we show how a set of conformally covariant invariants can be combined to produce a conformally covariant invariant that detects the event horizon of the conformally related black hole. As an application we consider the rotating dynamical black holes conformally related to the Kerr-Newman-Unti-Tamburino-(anti)-de Sitter spacetimes and construct an invariant that detects the conformal Killing horizon along with a second invariant that detects the conformal stationary limit surface. In addition, we present necessary conditions for a dynamical black hole to be conformally related to a stationary black hole and apply these conditions to the ingoing Kerr-Vaidya and Vaidya black hole solutions to determine if they are conformally related to stationary black holes for particular choices of the mass function. While two of the three approaches cannot be generalized to higher dimensions, we discuss the existence of a conformally covariant invariant that will detect the event horizon for any higher dimensional black hole conformally related to a stationary black hole which admits at least two conformally covariant invariants, including all vacuum spacetimes.

  20. Recognition of cis-acting sequences in RNA 3 of Prunus necrotic ringspot virus by the replicase of Alfalfa mosaic virus.

    Science.gov (United States)

    Aparicio, F; Sánchez-Navarro, J A; Olsthoorn, R C; Pallás, V; Bol, J F

    2001-04-01

    Alfalfa mosaic virus (AMV) and Prunus necrotic ringspot virus (PNRSV) belong to the genera ALFAMOVIRUS: and ILARVIRUS:, respectively, of the family BROMOVIRIDAE: Initiation of infection by AMV and PNRSV requires binding of a few molecules of coat protein (CP) to the 3' termini of the inoculum RNAs and the CPs of the two viruses are interchangeable in this early step of the replication cycle. CIS:-acting sequences in PNRSV RNA 3 that are recognized by the AMV replicase were studied in in vitro replicase assays and by inoculation of AMV-PNRSV RNA 3 chimeras to tobacco plants and protoplasts transformed with the AMV replicase genes (P12 plants). The results showed that the AMV replicase recognized the promoter for minus-strand RNA synthesis in PNRSV RNA 3 but not the promoter for plus-strand RNA synthesis. A chimeric RNA with PNRSV movement protein and CP genes accumulated in tobacco, which is a non-host for PNRSV.

  1. New geometries for black hole horizons

    Energy Technology Data Exchange (ETDEWEB)

    Armas, Jay [Physique Théorique et Mathématique,Université Libre de Bruxelles and International Solvay Institutes, ULB-Campus Plaine CP231, B-1050 Brussels (Belgium); Blau, Matthias [Albert Einstein Center for Fundamental Physics, University of Bern,Sidlerstrasse 5, 3012 Bern (Switzerland)

    2015-07-10

    We construct several classes of worldvolume effective actions for black holes by integrating out spatial sections of the worldvolume geometry of asymptotically flat black branes. This provides a generalisation of the blackfold approach for higher-dimensional black holes and yields a map between different effective theories, which we exploit by obtaining new hydrodynamic and elastic transport coefficients via simple integrations. Using Euclidean minimal surfaces in order to decouple the fluid dynamics on different sections of the worldvolume, we obtain local effective theories for ultraspinning Myers-Perry branes and helicoidal black branes, described in terms of a stress-energy tensor, particle currents and non-trivial boost vectors. We then study in detail and present novel compact and non-compact geometries for black hole horizons in higher-dimensional asymptotically flat space-time. These include doubly-spinning black rings, black helicoids and helicoidal p-branes as well as helicoidal black rings and helicoidal black tori in D≥6.

  2. Statistical mechanics of black holes

    International Nuclear Information System (INIS)

    Harms, B.; Leblanc, Y.

    1992-01-01

    We analyze the statistical mechanics of a gas of neutral and charged black holes. The microcanonical ensemble is the only possible approach to this system, and the equilibrium configuration is the one for which most of the energy is carried by a single black hole. Schwarzschild black holes are found to obey the statistical bootstrap condition. In all cases, the microcanonical temperature is identical to the Hawking temperature of the most massive black hole in the gas. U(1) charges in general break the bootstrap property. The problems of black-hole decay and of quantum coherence are also addressed

  3. Few-layer black phosphorus nanoparticles.

    Science.gov (United States)

    Sofer, Zdenek; Bouša, Daniel; Luxa, Jan; Mazanek, Vlastimil; Pumera, Martin

    2016-01-28

    Herein, black phosphorus quantum dots and nanoparticles of a few layer thickness were prepared and characterized using STEM, AFM, dynamic light scattering, X-ray photoelectron spectroscopy, X-ray diffraction, Raman spectroscopy and photoluminescence. Impact electrochemistry of the induvidual black phosphorus nanoparticles allows their size determination. The centrifugation of colloidal black phosphorus nanoparticles allowed separation of quantum dots with sizes up to 15 nm. These black phosphorus nanoparticles exhibit a large band gap and are expected to find a wide range of applications from semiconductors to biomolecule tags. The use of black phosphorus nanoparticles for vapour sensing was successfully demonstrated.

  4. Regular black hole in three dimensions

    OpenAIRE

    Myung, Yun Soo; Yoon, Myungseok

    2008-01-01

    We find a new black hole in three dimensional anti-de Sitter space by introducing an anisotropic perfect fluid inspired by the noncommutative black hole. This is a regular black hole with two horizons. We compare thermodynamics of this black hole with that of non-rotating BTZ black hole. The first-law of thermodynamics is not compatible with the Bekenstein-Hawking entropy.

  5. Boosted quantum black hole and black string in M-theory, and quantum correction to Gregory-Laflamme instability

    Energy Technology Data Exchange (ETDEWEB)

    Hyakutake, Yoshifumi [Faculty of Science, Ibaraki University,Bunkyo 2-1-1, Mito, Ibaraki, 310-8512 (Japan)

    2015-09-11

    We take into account higher derivative R{sup 4} corrections in M-theory and construct quantum black hole and black string solutions in 11 dimensions up to the next leading order. The quantum black string is stretching along the 11th direction and the Gregory-Laflamme instability is examined at the quantum level. Thermodynamics of the boosted quantum black hole and black string are also discussed. Especially we take the near horizon limit of the quantum black string and investigate its instability quantitatively.

  6. Molecular adaptation within the coat protein-encoding gene of Tunisian almond isolates of Prunus necrotic ringspot virus.

    Science.gov (United States)

    Boulila, Moncef; Ben Tiba, Sawssen; Jilani, Saoussen

    2013-04-01

    The sequence alignments of five Tunisian isolates of Prunus necrotic ringspot virus (PNRSV) were searched for evidence of recombination and diversifying selection. Since failing to account for recombination can elevate the false positive error rate in positive selection inference, a genetic algorithm (GARD) was used first and led to the detection of potential recombination events in the coat protein-encoding gene of that virus. The Recco algorithm confirmed these results by identifying, additionally, the potential recombinants. For neutrality testing and evaluation of nucleotide polymorphism in PNRSV CP gene, Tajima's D, and Fu and Li's D and F statistical tests were used. About selection inference, eight algorithms (SLAC, FEL, IFEL, REL, FUBAR, MEME, PARRIS, and GA branch) incorporated in HyPhy package were utilized to assess the selection pressure exerted on the expression of PNRSV capsid. Inferred phylogenies pointed out, in addition to the three classical groups (PE-5, PV-32, and PV-96), the delineation of a fourth cluster having the new proposed designation SW6, and a fifth clade comprising four Tunisian PNRSV isolates which underwent recombination and selective pressure and to which the name Tunisian outgroup was allocated.

  7. The Anti-Inflammatory Effect of Prunus yedoensis Bark Extract on Adipose Tissue in Diet-Induced Obese Mice

    Directory of Open Access Journals (Sweden)

    Hee Kang

    2015-01-01

    Full Text Available Chronic, low-grade inflammatory responses occur in obese adipose tissue and play a crucial role in the development of insulin resistance. Macrophages exposed to high glucose upregulate the expression of SRA, a macrophage-specific scavenger receptor. The present study investigated whether Prunus yedoensis (PY bark extract affects the inflammatory response and scavenger receptor gene expression observed in a diet-induced obesity model in vivo. Oral administration of PY extract significantly reduced fasting blood glucose levels without a change in body weight in mice fed a high fat diet for 17 weeks. PY extract significantly suppressed expression of inflammatory and macrophage genes such as tumor necrosis factor-α, interleukin-6, and F4/80 in epididymal adipose tissue. Among scavenger receptor genes, SRA expression was significantly reduced. The inhibitory responses of PY extract and its fractions were determined through evaluation of scavenger receptor expression in THP-1 cells. PY extract and its ethyl acetate fraction decreased the levels of SRA mRNA and phospho-ERK1/2 during monocyte differentiation. Our data indicate that the anti-inflammatory effects of PY extract and its downregulation of SRA seem to account for its hypoglycemic effects.

  8. Stationary black holes: large D analysis

    International Nuclear Information System (INIS)

    Suzuki, Ryotaku; Tanabe, Kentaro

    2015-01-01

    We consider the effective theory of large D stationary black holes. By solving the Einstein equations with a cosmological constant using the 1/D expansion in near zone of the black hole we obtain the effective equation for the stationary black hole. The effective equation describes the Myers-Perry black hole, bumpy black holes and, possibly, the black ring solution as its solutions. In this effective theory the black hole is represented as an embedded membrane in the background, e.g., Minkowski or Anti-de Sitter spacetime and its mean curvature is given by the surface gravity redshifted by the background gravitational field and the local Lorentz boost. The local Lorentz boost property of the effective equation is observed also in the metric itself. In fact we show that the leading order metric of the Einstein equation in the 1/D expansion is generically regarded as a Lorentz boosted Schwarzschild black hole. We apply this Lorentz boost property of the stationary black hole solution to solve perturbation equations. As a result we obtain an analytic formula for quasinormal modes of the singly rotating Myers-Perry black hole in the 1/D expansion.

  9. Black branes as piezoelectrics.

    Science.gov (United States)

    Armas, Jay; Gath, Jakob; Obers, Niels A

    2012-12-14

    We find a realization of linear electroelasticity theory in gravitational physics by uncovering a new response coefficient of charged black branes, exhibiting their piezoelectric behavior. Taking charged dilatonic black strings as an example and using the blackfold approach we measure their elastic and piezolectric moduli. We also use our results to draw predictions about the equilibrium condition of charged dilatonic black rings in dimensions higher than six.

  10. Black hole levitron

    NARCIS (Netherlands)

    Arsiwalla, X.D.; Verlinde, E.P.

    2010-01-01

    We study the problem of spatially stabilizing four dimensional extremal black holes in background electric/magnetic fields. Whilst looking for stationary stable solutions describing black holes placed in external fields we find that taking a continuum limit of Denef et al.’s multicenter

  11. Black Hole Grabs Starry Snack

    Science.gov (United States)

    2006-01-01

    [figure removed for brevity, see original site] Poster Version This artist's concept shows a supermassive black hole at the center of a remote galaxy digesting the remnants of a star. NASA's Galaxy Evolution Explorer had a 'ringside' seat for this feeding frenzy, using its ultraviolet eyes to study the process from beginning to end. The artist's concept chronicles the star being ripped apart and swallowed by the cosmic beast over time. First, the intact sun-like star (left) ventures too close to the black hole, and its own self-gravity is overwhelmed by the black hole's gravity. The star then stretches apart (middle yellow blob) and eventually breaks into stellar crumbs, some of which swirl into the black hole (cloudy ring at right). This doomed material heats up and radiates light, including ultraviolet light, before disappearing forever into the black hole. The Galaxy Evolution Explorer was able to watch this process unfold by observing changes in ultraviolet light. The area around the black hole appears warped because the gravity of the black hole acts like a lens, twisting and distorting light.

  12. A nonsingular rotating black hole

    International Nuclear Information System (INIS)

    Ghosh, Sushant G.

    2015-01-01

    The spacetime singularities in classical general relativity are inevitable, as predicated by the celebrated singularity theorems. However, it is a general belief that singularities do not exist in Nature and that they are the limitations of the general relativity. In the absence of a welldefined quantum gravity, models of regular black holes have been studied. We employ a probability distribution inspired mass function m(r) to replace the Kerr black hole mass M to represent a nonsingular rotating black hole that is identified asymptotically (r >> k, k > 0 constant) exactly as the Kerr-Newman black hole, and as the Kerr black hole when k = 0. The radiating counterpart renders a nonsingular generalization of Carmeli's spacetime as well as Vaidya's spacetime, in the appropriate limits. The exponential correction factor changing the geometry of the classical black hole to remove the curvature singularity can also be motivated by quantum arguments. The regular rotating spacetime can also be understood as a black hole of general relativity coupled to nonlinear electrodynamics. (orig.)

  13. Thermodynamic theory of black holes

    Energy Technology Data Exchange (ETDEWEB)

    Davies, P C.W. [King' s Coll., London (UK). Dept. of Mathematics

    1977-04-21

    The thermodynamic theory underlying black hole processes is developed in detail and applied to model systems. It is found that Kerr-Newman black holes undergo a phase transition at a = 0.68M or Q = 0.86M, where the heat capacity has an infinite discontinuity. Above the transition values the specific heat is positive, permitting isothermal equilibrium with a surrounding heat bath. Simple processes and stability criteria for various black hole situations are investigated. The limits for entropically favoured black hole formation are found. The Nernst conditions for the third law of thermodynamics are not satisfied fully for black holes. There is no obvious thermodynamic reason why a black hole may not be cooled down below absolute zero and converted into a naked singularity. Quantum energy-momentum tensor calculations for uncharged black holes are extended to the Reissner-Nordstrom case, and found to be fully consistent with the thermodynamic picture for Q < M. For Q < M the model predicts that 'naked' collapse also produces radiation, with such intensity that the collapsing matter is entirely evaporated away before a naked singularity can form.

  14. MULTIPLICAÇÃO IN VITRO DE PORTA-ENXERTOS DO GÊNERO PRUNUS SOB DIFERENTES CONCENTRAÇÕES DE BAP EM DOIS MEIOS DE CULTURA IN VITRO MULTIPLICATION OF PRUNUS ROOTSTOCKS IN DIFFERENT BAP CONCENTRATIONS IN TWO CULTURE MEDIA

    Directory of Open Access Journals (Sweden)

    CARLOS AUGUSTO POSSER SILVEIRA

    2001-12-01

    Full Text Available Este trabalho teve como objetivo determinar a melhor concentração de BAP (6-benzilaminopurina e meio de cultura para a multiplicação in vitro de porta-enxertos de Prunus sp., recentemente introduzidos no Brasil. Os porta-enxertos G x N22, GF 677, Mr.S 2/5, Marianna e Mirabolano foram testados em dois meios de cultura, meio MS e meio MS ¾ (reduzido em 25% dos sais do meio inteiro, combinados com quatro concentrações da citocinina BAP: 0,1; 0,3; 0,5 e 0,7 mg.L-1. Observou-se que os genótipos apresentaram comportamentos diferentes entre si em relação às concentrações de BAP e aos meios. Concluiu-se que, para os porta-enxertos G x N22 e Mr.S 2/5, o melhor meio de multiplicação é o MS ¾ com BAP na concentração de 0,7 mg.L-1; para o porta-enxerto Marianna, o meio MS com BAP na concentração de 0,7 mg.L-1; para o porta-enxerto Mirabolano, o meio MS ¾ com BAP na concentração de 0,5 mg.L-1, sendo que, para este porta-enxerto, o BAP exerce efeito negativo sobre o tamanho das brotações, independentemente do tipo de meio. Já para o porta-enxerto G x N22, este efeito se fez notar apenas no meio MS. O porta-enxerto GF 677 não apresentou bons resultados na propagação in vitro.The purpose of this work was to determine the best BAP concentration and culture media for the in vitro multiplication of Prunus rootstocks starting from nodal segments. The rootstocks G x N22, GF 677, Mr.S 2/5, Marianna and Mirabolano were tested in two culture media (MS and MS ¾ combined with four BAP concentrations (0.1; 0.3; 0.5 and 0.7 mg.L-1. It was observed that the genotypes had different performance as the two media and BAP concentrations are concerned. BAP provided better results in the MS ¾ for the rootstocks G x N22 and Mr.S 2/5. For 'Marianna', the best results were found in the MS at 0.7 mg.L-1 BAP. 'Mirabolano', showed better results in the MS ¾ at 0.5 mg.L-1 BAP and in the two tested media, the shoot length decreased with the increase for

  15. NASA Observatory Confirms Black Hole Limits

    Science.gov (United States)

    2005-02-01

    The very largest black holes reach a certain point and then grow no more, according to the best survey to date of black holes made with NASA's Chandra X-ray Observatory. Scientists have also discovered many previously hidden black holes that are well below their weight limit. These new results corroborate recent theoretical work about how black holes and galaxies grow. The biggest black holes, those with at least 100 million times the mass of the Sun, ate voraciously during the early Universe. Nearly all of them ran out of 'food' billions of years ago and went onto a forced starvation diet. Focus on Black Holes in the Chandra Deep Field North Focus on Black Holes in the Chandra Deep Field North On the other hand, black holes between about 10 and 100 million solar masses followed a more controlled eating plan. Because they took smaller portions of their meals of gas and dust, they continue growing today. "Our data show that some supermassive black holes seem to binge, while others prefer to graze", said Amy Barger of the University of Wisconsin in Madison and the University of Hawaii, lead author of the paper describing the results in the latest issue of The Astronomical Journal (Feb 2005). "We now understand better than ever before how supermassive black holes grow." One revelation is that there is a strong connection between the growth of black holes and the birth of stars. Previously, astronomers had done careful studies of the birthrate of stars in galaxies, but didn't know as much about the black holes at their centers. DSS Optical Image of Lockman Hole DSS Optical Image of Lockman Hole "These galaxies lose material into their central black holes at the same time that they make their stars," said Barger. "So whatever mechanism governs star formation in galaxies also governs black hole growth." Astronomers have made an accurate census of both the biggest, active black holes in the distance, and the relatively smaller, calmer ones closer by. Now, for the first

  16. Erratic Black Hole Regulates Itself

    Science.gov (United States)

    2009-03-01

    New results from NASA's Chandra X-ray Observatory have made a major advance in explaining how a special class of black holes may shut off the high-speed jets they produce. These results suggest that these black holes have a mechanism for regulating the rate at which they grow. Black holes come in many sizes: the supermassive ones, including those in quasars, which weigh in at millions to billions of times the mass of the Sun, and the much smaller stellar-mass black holes which have measured masses in the range of about 7 to 25 times the Sun's mass. Some stellar-mass black holes launch powerful jets of particles and radiation, like seen in quasars, and are called "micro-quasars". The new study looks at a famous micro-quasar in our own Galaxy, and regions close to its event horizon, or point of no return. This system, GRS 1915+105 (GRS 1915 for short), contains a black hole about 14 times the mass of the Sun that is feeding off material from a nearby companion star. As the material swirls toward the black hole, an accretion disk forms. This system shows remarkably unpredictable and complicated variability ranging from timescales of seconds to months, including 14 different patterns of variation. These variations are caused by a poorly understood connection between the disk and the radio jet seen in GRS 1915. Chandra, with its spectrograph, has observed GRS 1915 eleven times since its launch in 1999. These studies reveal that the jet in GRS 1915 may be periodically choked off when a hot wind, seen in X-rays, is driven off the accretion disk around the black hole. The wind is believed to shut down the jet by depriving it of matter that would have otherwise fueled it. Conversely, once the wind dies down, the jet can re-emerge. "We think the jet and wind around this black hole are in a sort of tug of war," said Joseph Neilsen, Harvard graduate student and lead author of the paper appearing in the journal Nature. "Sometimes one is winning and then, for reasons we don

  17. Atypical features of hyperthyroidism in Blacks

    International Nuclear Information System (INIS)

    Kalk, W.J.

    1980-01-01

    Hyperthyroidism is reportedly uncommon in the indigenous populations of Africa. The presenting symptoms volunteered, the symptoms elicited by direct questioning, and the results of physical examination were therefore prospectively compared in 60 Black and 56 White patients with thyrotoxicosis attending a single thyroid clinic. Fewer Blacks than Whites volunteered information about weight loss, while more Blacks complained only of the presence of a goitre. A 'chance' diagnosis of hyperthyroidism was made more frequently in Blacks. Symptomatology elicited by direct questioning and findings on physical examination were generally similar in each group, except that Blacks presented more frequently with complicated disease (cardiac failure and overt myopathy) and infiltrative ophthalmopathy. The frequency with which hyperthyroidism presents 'atypically' in Black compared with White patients may reflect educational, socio-economic and cultural differences in the Black and White populations, and may partly explain the infrequency with which this disease is diagnosed in Blacks

  18. Black layers on historical architecture.

    Science.gov (United States)

    Toniolo, Lucia; Zerbi, Carlotta M; Bugini, Roberto

    2009-03-01

    The external surface of any building in urban polluted environment is unavoidably destined to be covered with layers that assume a grey to black colour and are generally called 'black crusts'. These, according to standard protocols and glossary, are deteriorated surface layers of stone material; they can have variable thickness, are hard and fragile and can detach spontaneously from the substrate, which, in general, is quite decayed. Plain visual examination may lead to consider 'black crusts' all similar, whilst only a careful diagnostic investigation can distinguish 'black crusts' and the consequences of their formation on stone substrates. In this paper, various black layers on marble are studied and compared and the morphological and compositional characteristics discussed according to the related mechanisms of formation. Differences between old (hundred years) and recent crusts (30 years) are investigated and pointed out. Samples of black crusts collected from the Milan Cathedral façade (Candoglia Marble) have been studied and compared with the careful and synergic employ of traditional techniques: optical (transmission and reflected VIS light) and electron microscopy, X-ray spectrometry and micro-Fourier transform infrared spectroscopy. Visual examination of loose fragments does not allow to point out outstanding differences amongst the various samples; black layers have similar main mineral components, gypsum and airborne particles, with different spatial distribution. The microscopic studies allowed to point out the porosity differences, the gypsum crystallisation habit, different amount of embedded particles, level and progress of marble decay. The observations lead to define three main types of black crusts: black crust deriving from marble sulphation, compact deposit and encrustation due to exogenic materials deposition. Black crusts show evidence of sulphation in progress, without a clear continuity solution between crust and marble; the lack of

  19. The importance of bee pollination of the sour cherry (Prunus cerasus Cultivar ‘Stevnsbaer’ in Denmark

    Directory of Open Access Journals (Sweden)

    Lise Hansted

    2012-12-01

    Full Text Available Low fruit set, despite normally-developed flowers, is often a significant contributor to poor yield of the self-fertile sour cherry (Prunus cerasus cultivar ‘Stevnsbaer’ in Denmark. The aim of this study was to investigate the effect of insect, and particularly, bee pollination on the fruit set of this cultivar, in order to provide orchard management information for both Danish ‘Stevnsbaer’ growers and beekeepers. Visits to cherry flowers by honey bees (Apis mellifera, Bombus species and solitary bees, were recorded during the flowering of ‘Stevnsbaer’ in five separate Danish orchards. The results indicate that there is a significantly higher fruit set on open pollinated branches when compared to caged branches, where bees and other pollinating insects where excluded. The results were qualitatively consistent over three different seasons (2007, 2009 and 2010. A period of prolonged cold, humid weather before and during early flowering probably reduced fruit set significantly in 2010 compared to 2009. Regarding the apparent benefits of bee pollination on fruit set and subsequent implications for yield, we recommend placing honeybees in ‘Stevnsbaer’ orchards during flowering to sustain commercially viable production. Another valuable management strategy would be to improve foraging and nesting conditions to support both honey and wild bees in and around the orchards.

  20. Nonsingular black hole

    Energy Technology Data Exchange (ETDEWEB)

    Chamseddine, Ali H. [American University of Beirut, Physics Department, Beirut (Lebanon); I.H.E.S., Bures-sur-Yvette (France); Mukhanov, Viatcheslav [Niels Bohr Institute, Niels Bohr International Academy, Copenhagen (Denmark); Ludwig-Maximilians University, Theoretical Physics, Munich (Germany); MPI for Physics, Munich (Germany)

    2017-03-15

    We consider the Schwarzschild black hole and show how, in a theory with limiting curvature, the physical singularity ''inside it'' is removed. The resulting spacetime is geodesically complete. The internal structure of this nonsingular black hole is analogous to Russian nesting dolls. Namely, after falling into the black hole of radius r{sub g}, an observer, instead of being destroyed at the singularity, gets for a short time into the region with limiting curvature. After that he re-emerges in the near horizon region of a spacetime described by the Schwarzschild metric of a gravitational radius proportional to r{sub g}{sup 1/3}. In the next cycle, after passing the limiting curvature, the observer finds himself within a black hole of even smaller radius proportional to r{sub g}{sup 1/9}, and so on. Finally after a few cycles he will end up in the spacetime where he remains forever at limiting curvature. (orig.)

  1. Black Teenage Pregnancy

    Directory of Open Access Journals (Sweden)

    Loretta I. Winters

    2012-01-01

    Full Text Available This article examines the relative importance of race and socioeconomic status (SES in determining whether Black and White teenagers report having ever been pregnant. Data gathered from 1999 to 2006 by the National Center for Health Statistics of the Center for Disease Control and Prevention included 1,580 Black and White females aged 15 to 19 years. Results supported the effects of race and SES, with SES having the stronger effect. However, the effects of race and SES differ when controlling for the state of the economy. No difference between Blacks and Whites was found during better economic times. During 2003-2004, the period of greatest economic stress, race was determined to be the only predictor of teenage pregnancy. In particular, during 2005-2006, the reduction in pregnancy rates for Black minors (15-17 fell below those for White minors within their respective SES categories. Policy implications are discussed in light of these findings.

  2. "What Exactly Is a Black?": Interrogating the Reality of Race in Jean Genet's The Blacks

    Directory of Open Access Journals (Sweden)

    Debby Thompson

    2002-06-01

    Full Text Available On the dedication page of The Blacks , Genet writes "One evening an actor asked me to write a play for an all-black cast. But what exactly is a black? First of all, what's his color?" Prefiguring major issues and paradoxes of African American cultural studies today, The Blacks insists on the very real ways in which the black/white racial binary, like the very concept of race itself, is lived and socially enforced, and at the same time argues that the binary is ultimately a fiction, made real through performative reification. Genet's "clown show," ambiguously reversing the blackface minstrelsy tradition, dramatizes how racial identity can become internalized and naturalized through cultural myths of interracial rape and colonialist narratives of a "heart of darkness," an imaginary site from which "black essence" arises. These racist performance traditions and narratives are so powerful and resilient that attempts to protest them become subtle and unwitting ways of re-creating them. The Blacks' parody of the minstrel tradition to interrogate all racial identity is so permanently suspended in a dialectic of both reinforcing the black/white binary and invalidating it, that the play's strategies present vital paradigms for the study of other and diverse interrogations of racial ontologies.

  3. Identité et marchandisation : Le cas des black memorabilia et black collectibles Identity and Commodification: The Case of Black Memorabilia and Black Collectibles

    Directory of Open Access Journals (Sweden)

    Eliane Elmaleh

    2009-07-01

    Full Text Available The aim of this article is to raise the issue of the commodification of African American culture, mainly through a certain form of art that has re-emerged with Black memorabilia which paradoxically fit into a black-driven market. However, these items penetrated American culture from 1920 to the 1950s to convey images of black people as lazy, stupid, childlike and happy. This condition of permanent happiness, typified by a broad smile and white teeth, was a fundamental component of this racist and stereotyped imagery. Thus, this article focuses on the thin border between racism and the denunciation of racism, commodification and denunciation of commodification, art and its by-products being always co-opted in the specific logic of private productivity.

  4. Black Girl Cartography: Black Girlhood and Place-Making in Education Research

    Science.gov (United States)

    Butler, Tamara T.

    2018-01-01

    Drawing on research in education, Black Girlhood studies, and conversations connected to girlhood and cartography, this chapter calls for transdisciplinary analyses of Black girls' sociocultural and geopolitical locations in education research. In reviewing education research documenting the practices and interrogating the experiences of Black…

  5. Axion-dilation black holes

    International Nuclear Information System (INIS)

    Kallosh, R.

    1993-01-01

    In this talk some essential features of stringy black holes are described. The author considers charged U(1) and U(1) x U(1) four-dimensional axion-dilaton black holes. The Hawking temperature and the entropy of all solutions are shown to be simple functions of the squares of supercharges, defining the positivity bounds. Spherically symmetric and multi black hole solutions are presented. The extreme solutions with zero entropy (holons) represent a ground state of the theory and are characterized by elementary dilaton, axion, electric, and magnetic charges. The attractive gravitational and axion-dilaton force is balanced by the repulsive electromagnetic force. The author discusses the possibility of splitting of nearly extreme black holes. 11 refs

  6. Black History, Inc! Investigating the Production of Black History through Walmart's Corporate Web Site

    Science.gov (United States)

    King, LaGarrett J.; Brown, Anthony L.

    2012-01-01

    Social and public sites are becoming a popular medium for intellectual consumption of Black history. Given the educational climate in which many students' exposure to Black history may come from outside of schools, the authors examine how Walmart's Black History Month Web site produced simplistic and safe narratives about African American history.

  7. What Can We Learn About Black-Hole Formation from Black-Hole X-ray Binaries?

    NARCIS (Netherlands)

    Nelemans, G.A.

    2007-01-01

    I discuss the effect of the formation of a black hole on a (close) binary and show some of the current constraints that the observed properties of black hole X-ray binaries put on the formation of black holes. In particular, I discuss the evidence for and against asymmetric kicks imparted on the

  8. Why Are Black Employers More Likely Than White Employers To Hire Blacks? Discussion Paper.

    Science.gov (United States)

    Stoll, Michael A.; Raphael, Steven; Holzer, Harry J.

    This study investigated why black employers tend to hire blacks at higher rates than do white employers and examined individual steps in the hiring process, the role of the hiring agent's race, and the degree to which variation in black application rates related to differences in observable characteristics, such as an establishment's physical…

  9. Black holes and the multiverse

    International Nuclear Information System (INIS)

    Garriga, Jaume; Vilenkin, Alexander; Zhang, Jun

    2016-01-01

    Vacuum bubbles may nucleate and expand during the inflationary epoch in the early universe. After inflation ends, the bubbles quickly dissipate their kinetic energy; they come to rest with respect to the Hubble flow and eventually form black holes. The fate of the bubble itself depends on the resulting black hole mass. If the mass is smaller than a certain critical value, the bubble collapses to a singularity. Otherwise, the bubble interior inflates, forming a baby universe, which is connected to the exterior FRW region by a wormhole. A similar black hole formation mechanism operates for spherical domain walls nucleating during inflation. As an illustrative example, we studied the black hole mass spectrum in the domain wall scenario, assuming that domain walls interact with matter only gravitationally. Our results indicate that, depending on the model parameters, black holes produced in this scenario can have significant astrophysical effects and can even serve as dark matter or as seeds for supermassive black holes. The mechanism of black hole formation described in this paper is very generic and has important implications for the global structure of the universe. Baby universes inside super-critical black holes inflate eternally and nucleate bubbles of all vacua allowed by the underlying particle physics. The resulting multiverse has a very non-trivial spacetime structure, with a multitude of eternally inflating regions connected by wormholes. If a black hole population with the predicted mass spectrum is discovered, it could be regarded as evidence for inflation and for the existence of a multiverse

  10. Black holes and the multiverse

    Energy Technology Data Exchange (ETDEWEB)

    Garriga, Jaume [Departament de Fisica Fonamental i Institut de Ciencies del Cosmos, Universitat de Barcelona, Marti i Franques, 1, Barcelona, 08028 Spain (Spain); Vilenkin, Alexander; Zhang, Jun, E-mail: jaume.garriga@ub.edu, E-mail: vilenkin@cosmos.phy.tufts.edu, E-mail: jun.zhang@tufts.edu [Institute of Cosmology, Tufts University, 574 Boston Ave, Medford, MA, 02155 (United States)

    2016-02-01

    Vacuum bubbles may nucleate and expand during the inflationary epoch in the early universe. After inflation ends, the bubbles quickly dissipate their kinetic energy; they come to rest with respect to the Hubble flow and eventually form black holes. The fate of the bubble itself depends on the resulting black hole mass. If the mass is smaller than a certain critical value, the bubble collapses to a singularity. Otherwise, the bubble interior inflates, forming a baby universe, which is connected to the exterior FRW region by a wormhole. A similar black hole formation mechanism operates for spherical domain walls nucleating during inflation. As an illustrative example, we studied the black hole mass spectrum in the domain wall scenario, assuming that domain walls interact with matter only gravitationally. Our results indicate that, depending on the model parameters, black holes produced in this scenario can have significant astrophysical effects and can even serve as dark matter or as seeds for supermassive black holes. The mechanism of black hole formation described in this paper is very generic and has important implications for the global structure of the universe. Baby universes inside super-critical black holes inflate eternally and nucleate bubbles of all vacua allowed by the underlying particle physics. The resulting multiverse has a very non-trivial spacetime structure, with a multitude of eternally inflating regions connected by wormholes. If a black hole population with the predicted mass spectrum is discovered, it could be regarded as evidence for inflation and for the existence of a multiverse.

  11. SEP-class genes in Prunus mume and their likely role in floral organ development.

    Science.gov (United States)

    Zhou, Yuzhen; Xu, Zongda; Yong, Xue; Ahmad, Sagheer; Yang, Weiru; Cheng, Tangren; Wang, Jia; Zhang, Qixiang

    2017-01-13

    Flower phylogenetics and genetically controlled development have been revolutionised during the last two decades. However, some of these evolutionary aspects are still debatable. MADS-box genes are known to play essential role in specifying the floral organogenesis and differentiation in numerous model plants like Petunia hybrida, Arabidopsis thaliana and Antirrhinum majus. SEPALLATA (SEP) genes, belonging to the MADS-box gene family, are members of the ABCDE and quartet models of floral organ development and play a vital role in flower development. However, few studies of the genes in Prunus mume have yet been conducted. In this study, we cloned four PmSEPs and investigated their phylogenetic relationship with other species. Expression pattern analyses and yeast two-hybrid assays of these four genes indicated their involvement in the floral organogenesis with PmSEP4 specifically related to specification of the prolificated flowers in P. mume. It was observed that the flower meristem was specified by PmSEP1 and PmSEP4, the sepal by PmSEP1 and PmSEP4, petals by PmSEP2 and PmSEP3, stamens by PmSEP2 and PmSEP3 and pistils by PmSEP2 and PmSEP3. With the above in mind, flower development in P. mume might be due to an expression of SEP genes. Our findings can provide a foundation for further investigations of the transcriptional factors governing flower development, their molecular mechanisms and genetic basis.

  12. Dual role of imidazole as activator/inhibitor of sweet almond (Prunus dulcis β-glucosidase

    Directory of Open Access Journals (Sweden)

    Sara Caramia

    2017-07-01

    Full Text Available The activity of Prunus dulcis (sweet almond β-glucosidase at the expense of p-nitrophenyl-β-d-glucopyranoside at pH 6 was determined, both under steady-state and pre-steady-state conditions. Using crude enzyme preparations, competitive inhibition by 1–5 mM imidazole was observed under both kinetic conditions tested. However, when imidazole was added to reaction mixtures at 0.125–0.250 mM, we detected a significant enzyme activation. To further inspect this effect exerted by imidazole, β-glucosidase was purified to homogeneity. Two enzyme isoforms were isolated, i.e. a full-length monomer, and a dimer containing a full-length and a truncated subunit. Dimeric β-glucosidase was found to perform much better than the monomeric enzyme, independently of the kinetic conditions used to assay enzyme activity. In addition, the sensitivity towards imidazole was found to differ between the two isoforms. While monomeric enzyme was indeed found to be relatively insensitive to imidazole, dimeric β-glucosidase was observed to be significantly activated by 0.125–0.250 mM imidazole under pre-steady-state conditions. Further, steady-state assays revealed that the addition of 0.125 mM imidazole to reaction mixtures increases the Km of dimeric enzyme from 2.3 to 6.7 mM. The activation of β-glucosidase dimer by imidazole is proposed to be exerted via a conformational transition poising the enzyme towards proficient catalysis.

  13. Dual role of imidazole as activator/inhibitor of sweet almond (Prunus dulcis) β-glucosidase.

    Science.gov (United States)

    Caramia, Sara; Gatius, Angela Gala Morena; Dal Piaz, Fabrizio; Gaja, Denis; Hochkoeppler, Alejandro

    2017-07-01

    The activity of Prunus dulcis (sweet almond) β-glucosidase at the expense of p -nitrophenyl-β-d-glucopyranoside at pH 6 was determined, both under steady-state and pre-steady-state conditions. Using crude enzyme preparations, competitive inhibition by 1-5 mM imidazole was observed under both kinetic conditions tested. However, when imidazole was added to reaction mixtures at 0.125-0.250 mM, we detected a significant enzyme activation. To further inspect this effect exerted by imidazole, β-glucosidase was purified to homogeneity. Two enzyme isoforms were isolated, i.e. a full-length monomer, and a dimer containing a full-length and a truncated subunit. Dimeric β-glucosidase was found to perform much better than the monomeric enzyme, independently of the kinetic conditions used to assay enzyme activity. In addition, the sensitivity towards imidazole was found to differ between the two isoforms. While monomeric enzyme was indeed found to be relatively insensitive to imidazole, dimeric β-glucosidase was observed to be significantly activated by 0.125-0.250 mM imidazole under pre-steady-state conditions. Further, steady-state assays revealed that the addition of 0.125 mM imidazole to reaction mixtures increases the K m of dimeric enzyme from 2.3 to 6.7 mM. The activation of β-glucosidase dimer by imidazole is proposed to be exerted via a conformational transition poising the enzyme towards proficient catalysis.

  14. In vitro evidence for RNA binding properties of the coat protein of prunus necrotic ringspot ilarvirus and their comparison to related and unrelated viruses.

    Science.gov (United States)

    Pallás, V; Sánchez-Navarro, J A; Díez, J

    1999-01-01

    The RNA binding properties of the prunus necrotic ringspot virus (PNRSV) coat protein (CP) were demonstrated by northwestern and dot-blot analyses. The capability to bind PNRSV RNA 4 was compared with viruses representing three different interactions prevailing in the assembly and architecture of virions. The results showed that cucumber mosaic virus (CMV) and PNRSV CPs, which stabilise their virions mainly through RNA-protein interactions bound PNRSV RNA 4 even at very high salt concentrations. The CP of cherry leaf roll nepovirus, whose virions are predominantly stabilised by protein-protein interactions did not bind even at the lowest salt concentration tested. Finally the CP of carnation mottle carmovirus, that has an intermediate position in which both RNA-protein and protein-protein interactions are equally important showed a salt-dependent RNA binding.

  15. Super-horizon primordial black holes

    International Nuclear Information System (INIS)

    Harada, Tomohiro; Carr, B.J.

    2005-01-01

    We discuss a new class of solutions to the Einstein equations which describe a primordial black hole (PBH) in a flat Friedmann background. Such solutions arise if a Schwarzschild black hole is patched onto a Friedmann background via a transition region. They are possible providing the black hole event horizon is larger than the cosmological apparent horizon. Such solutions have a number of strange features. In particular, one has to define the black hole and cosmological horizons carefully and one then finds that the mass contained within the black hole event horizon decreases when the black hole is larger than the Friedmann cosmological apparent horizon, although its area always increases. These solutions involve two distinct future null infinities and are interpreted as the conversion of a white hole into a black hole. Although such solutions may not form from gravitational collapse in the same way as standard PBHs, there is nothing unphysical about them, since all energy and causality conditions are satisfied. Their conformal diagram is a natural amalgamation of the Kruskal diagram for the extended Schwarzschild solution and the conformal diagram for a black hole in a flat Friedmann background. In this paper, such solutions are obtained numerically for a spherically symmetric universe containing a massless scalar field, but it is likely that they exist for more general matter fields and less symmetric systems

  16. THE BITTER TRUTH: A COMPARATIVE ANALYSIS OF BLACK MALE AND BLACK FEMALE ENTREPRENEURS

    OpenAIRE

    SHERRHONDA R. GIBBS

    2014-01-01

    The paper examines variables stated to be central to entrepreneurial success and reports differences between black male and black female entrepreneurs. Variables studied include task-specific efficacy, firm performance and opportunity recognition. Using a sample of 85 males and 58 females, results indicated that although black male and female entrepreneurs are somewhat similar in terms of business environment and demographics (e.g., education levels, years of business industry experience, lik...

  17. When Supermassive Black Holes Wander

    Science.gov (United States)

    Kohler, Susanna

    2018-05-01

    Are supermassive black holes found only at the centers of galaxies? Definitely not, according to a new study in fact, galaxies like the Milky Way may harbor several such monsters wandering through their midst.Collecting Black Holes Through MergersIts generally believed that galaxies are built up hierarchically, growing in size through repeated mergers over time. Each galaxy in a major merger likely hosts a supermassive black hole a black hole of millions to billions of times the mass of the Sun at its center. When a pair of galaxies merges, their supermassive black holes will often sink to the center of the merger via a process known as dynamical friction. There the supermassive black holes themselves will eventually merge in a burst of gravitational waves.Spatial distribution and velocities of wandering supermassive black holes in three of the authors simulated galaxies, shown in edge-on (left) and face-on (right) views of the galaxy disks. Click for a closer look. [Tremmel et al. 2018]But if a galaxy the size of the Milky Way was built through a history of many major galactic mergers, are we sure that all its accumulated supermassive black holes eventually merged at the galactic center? A new study suggests that some of these giants might have escaped such a fate and they now wander unseen on wide orbits through their galaxies.Black Holes in an Evolving UniverseLed by Michael Tremmel (Yale Center for Astronomy Astrophysics), a team of scientists has used data from a large-scale cosmological simulation, Romulus25, to explore the possibility of wandering supermassive black holes. The Romulus simulations are uniquely suited to track the formation and subsequent orbital motion of supermassive black holes as galactic halos are built up through mergers over the history of the universe.From these simulations, Tremmel and collaborators find an end total of 316 supermassive black holes residing within the bounds of 26 Milky-Way-mass halos. Of these, roughly a third are

  18. Black powder in gas pipelines

    Energy Technology Data Exchange (ETDEWEB)

    Sherik, Abdelmounam [Saudi Aramco, Dhahran (Saudi Arabia)

    2009-07-01

    Despite its common occurrence in the gas industry, black powder is a problem that is not well understood across the industry, in terms of its chemical and physical properties, source, formation, prevention or management of its impacts. In order to prevent or effectively manage the impacts of black powder, it is essential to have knowledge of its chemical and physical properties, formation mechanisms and sources. The present paper is divided into three parts. The first part of this paper is a synopsis of published literature. The second part reviews the recent laboratory and field work conducted at Saudi Aramco Research and Development Center to determine the compositions, properties, sources and formation mechanisms of black powder in gas transmission systems. Microhardness, nano-indentation, X-ray Diffraction (XRD), X-ray Fluorescence (XRF) and Scanning Electron Microscopy (SEM) techniques were used to analyze a large number of black powder samples collected from the field. Our findings showed that black powder is generated inside pipelines due to internal corrosion and that the composition of black powder is dependent on the composition of transported gas. The final part presents a summary and brief discussion of various black powder management methods. (author)

  19. Anyon black holes

    Science.gov (United States)

    Aghaei Abchouyeh, Maryam; Mirza, Behrouz; Karimi Takrami, Moein; Younesizadeh, Younes

    2018-05-01

    We propose a correspondence between an Anyon Van der Waals fluid and a (2 + 1) dimensional AdS black hole. Anyons are particles with intermediate statistics that interpolates between a Fermi-Dirac statistics and a Bose-Einstein one. A parameter α (0 quasi Fermi-Dirac statistics for α >αc, but a quasi Bose-Einstein statistics for α quasi Bose-Einstein statistics. For α >αc and a range of values of the cosmological constant, there is, however, no event horizon so there is no black hole solution. Thus, for these values of cosmological constants, the AdS Anyon Van der Waals black holes have only quasi Bose-Einstein statistics.

  20. Black-hole driven winds

    International Nuclear Information System (INIS)

    Punsly, B.M.

    1988-01-01

    This dissertation is a study of the physical mechanism that allows a large scale magnetic field to torque a rapidly rotating, supermassive black hole. This is an interesting problem as it has been conjectured that rapidly rotating black holes are the central engines that power the observed extragalactic double radio sources. Axisymmetric solutions of the curved space-time version of Maxwell's equations in the vacuum do not torque black holes. Plasma must be introduced for the hole to mechanically couple to the field. The dynamical aspect of rotating black holes that couples the magnetic field to the hole is the following. A rotating black hole forces the external geometry of space-time to rotate (the dragging of inertial frames). Inside of the stationary limit surface, the ergosphere, all physical particle trajectories must appear to rotate in the same direction as the black hole as viewed by the stationary observers at asymptotic infinity. In the text, it is demonstrated how plasma that is created on field lines that thread both the ergosphere and the equatorial plane will be pulled by gravity toward the equator. By the aforementioned properties of the ergosphere, the disk must rotate. Consequently, the disk acts like a unipolar generator. It drives a global current system that supports the toroidal magnetic field in an outgoing, magnetically dominated wind. This wind carries energy (mainly in the form of Poynting flux) and angular momentum towards infinity. The spin down of the black hole is the ultimate source of this energy and angular momentum flux

  1. ULTRAMASSIVE BLACK HOLE COALESCENCE

    International Nuclear Information System (INIS)

    Khan, Fazeel Mahmood; Holley-Bockelmann, Kelly; Berczik, Peter

    2015-01-01

    Although supermassive black holes (SMBHs) correlate well with their host galaxies, there is an emerging view that outliers exist. Henize 2-10, NGC 4889, and NGC 1277 are examples of SMBHs at least an order of magnitude more massive than their host galaxy suggests. The dynamical effects of such ultramassive central black holes is unclear. Here, we perform direct N-body simulations of mergers of galactic nuclei where one black hole is ultramassive to study the evolution of the remnant and the black hole dynamics in this extreme regime. We find that the merger remnant is axisymmetric near the center, while near the large SMBH influence radius, the galaxy is triaxial. The SMBH separation shrinks rapidly due to dynamical friction, and quickly forms a binary black hole; if we scale our model to the most massive estimate for the NGC 1277 black hole, for example, the timescale for the SMBH separation to shrink from nearly a kiloparsec to less than a parsec is roughly 10 Myr. By the time the SMBHs form a hard binary, gravitational wave emission dominates, and the black holes coalesce in a mere few Myr. Curiously, these extremely massive binaries appear to nearly bypass the three-body scattering evolutionary phase. Our study suggests that in this extreme case, SMBH coalescence is governed by dynamical friction followed nearly directly by gravitational wave emission, resulting in a rapid and efficient SMBH coalescence timescale. We discuss the implications for gravitational wave event rates and hypervelocity star production

  2. "Iron-Clad" Evidence For Spinning Black Hole

    Science.gov (United States)

    2003-09-01

    Telltale X-rays from iron may reveal if black holes are spinning or not, according to astronomers using NASA's Chandra X-ray Observatory and the European Space Agency's XMM-Newton Observatory. The gas flows and bizarre gravitational effects observed near stellar black holes are similar to those seen around supermassive black holes. Stellar black holes, in effect, are convenient `scale models' of their much larger cousins. Black holes come in at least two different sizes. Stellar black holes are between five and 20 times the mass of the Sun. At the other end of the size scale, supermassive black holes contain millions or billions times the mass of our Sun. The Milky Way contains both a supermassive black hole at its center, as well as a number of stellar black holes sprinkled throughout the Galaxy. At a press conference at the "Four Years of Chandra" symposium in Huntsville, Ala., Jon Miller of the Harvard-Smithsonian Center for Astrophysics in Cambridge, Mass. discussed recent results on the X-ray spectra, or distribution of X-rays with energy, from the iron atoms in gas around three stellar black holes in the Milky Way. "Discovering the high degree of correspondence between stellar and supermassive black holes is a real breakthrough," said Miller. "Because stellar black holes are smaller, everything happens about a million times faster, so they can be used as a test-bed for theories of how spinning black holes affect the space and matter around them." X-rays from a stellar black hole are produced when gas from a nearby companion star is heated to tens of millions of degrees as it swirls toward the black hole. Iron atoms in this gas produce distinctive X-ray signals that can be used to study the orbits of particles around the black hole. For example, the gravity of a black hole can shift the X-rays to lower energies. "The latest work provides the most precise measurements yet of the X-ray spectra for stellar black holes," said Miller. "These data help rule out

  3. Protecting Black Girls

    Science.gov (United States)

    Morris, Monique W.

    2016-01-01

    Statistics show that black girls in U.S. K-12 public schools are overrepresented among students who face disciplinary approaches (such as suspensions) that exclude or even criminalize them. Morris explains how black girls face conditions that make them vulnerable to a phenomenon she calls "school to confinement pathways"--conditions like…

  4. Black holes a very short introduction

    CERN Document Server

    Blundell, Katherine

    2015-01-01

    Black holes are a constant source of fascination to many due to their mysterious nature. Black Holes: A Very Short Introduction addresses a variety of questions, including what a black hole actually is, how they are characterized and discovered, and what would happen if you came too close to one. It explains how black holes form and grow—by stealing material that belongs to stars—as well as how many there may be in the Universe. It also explores the large black holes found in the centres of galaxies, and how black holes power quasars and lie behind other spectacular phenomena in the cosmos.

  5. Dye-sensitized solar cells fabricated with black raspberry, black carrot and rosella juice

    Science.gov (United States)

    Tekerek, S.; Kudret, A.; Alver, Ü.

    2011-10-01

    In this work, dye sensitized solar cells (DSSC's) were constructed from black raspberry ( Rubus Ideaus), black carrot ( Daucuscarota L.) and rosella juice ( Hibiscus Sabdariffa L.). In order to fabricate a DSSC the fluorine-doped tin (IV) oxide (FTO) thin films obtained by using spray pyrolysis technique were used as a substrate. TiO2 films on FTO layers were prepared by doctor-blading technique. Platinum-coated counter electrode and liquid Iodide/Iodine electrolyte solution were used to fabricate DSSC's. The efficiencies of solar cells produced with black carrot, rosella and black raspberry juice were calculated as 0.25%, 0.16% and 0.16% respectively, under a sunny day in Kahramanmaraş-Turkey.

  6. Familial Influences on Poverty Among Young Children in Black Immigrant, U.S.-born Black, and Nonblack Immigrant Families

    Science.gov (United States)

    Thomas, Kevin J. A.

    2014-01-01

    This study examines how familial contexts affect poverty disparities between the children of immigrant and U.S.-born blacks, and among black and nonblack children of immigrants. Despite lower gross child poverty rates in immigrant than in U.S.-born black families, accounting for differences in family structure reveals that child poverty risks among blacks are highest in single-parent black immigrant families. In addition, within two-parent immigrant families, child poverty declines associated with increasing assimilation are greater than the respective declines in single-parent families. The heads of black immigrant households have more schooling than those of native-black households. However, increased schooling has a weaker negative association with child poverty among the former than among the latter. In terms of racial disparities among the children of immigrants, poverty rates are higher among black than nonblack children. This black disadvantage is, however, driven by the outcomes of first-generation children of African and Hispanic-black immigrants. The results also show that although children in refugee families face elevated poverty risks, these risks are higher among black than among nonblack children of refugees. In addition, the poverty-reducing impact associated with having an English-proficient household head is about three times lower among black children of immigrants than among non-Hispanic white children of immigrants. PMID:21491186

  7. Graft-copolymerization onto carbon black

    International Nuclear Information System (INIS)

    Nakase, Yoshiaki; Nishii, Masanobu; Kijima, Toshiyuki; Kato, Hiroshi.

    1988-07-01

    Radiation-induced graft copolymerization of vinyl monomer onto carbon black was performed. During the γ-ray- and electron beam-induced polymerization (In-source), or the electron beam post-polymerization, the graft-copolymerization behavior was affected by the kinds of both carbon blacks and monomers, i.e. the smaller the size of carbon black particles, the higher the apparent grafted fraction. Homopolymer in the grafted carbon black samples was washed out by the solvent of the polymer, and the extracted polymer seemed to be dimer or trimer of the used monomer. In the case of the post-polymerization with the pre-irradiation doses of 50 Mrad, homopolymer was hardly observed. The polymer sheets of plastics or rubbers with grafted carbon black had an electrical conductivity unalterable considerably by the heating cycles. The particles of grafted carbon black in the sheet might be kept much more at the surface layer within 100 nm depth than at the inner layer. (author)

  8. Particle creation by black holes

    International Nuclear Information System (INIS)

    Hawking, S.W.

    1975-01-01

    In the classical theory black holes can only absorb and not emit particles. However it is shown that quantum mechanical effects cause black holes to create and emit particles. This thermal emission leads to a slow decrease in the mass of the black hole and to its eventual disappearance: any primordial black hole of mass less than about 10 15 g would have evaporated by now. Although these quantum effects violate the classical law that the area of the event horizon of a black hole cannot decrease, there remains a Generalized Second Law: S + 1/4 A never decreases where S is the entropy of matter outside black holes and A is the sum of the surface areas of the event horizons. This shows that gravitational collapse converts the baryons and leptons in the collapsing body into entropy. It is tempting to speculate that this might be the reason why the Universe contains so much entropy per baryon. (orig.) [de

  9. Black Hole's 1/N Hair

    CERN Document Server

    Dvali, Gia

    2013-01-01

    According to the standard view classically black holes carry no hair, whereas quantum hair is at best exponentially weak. We show that suppression of hair is an artifact of the semi-classical treatment and that in the quantum picture hair appears as an inverse mass-square effect. Such hair is predicted in the microscopic quantum description in which a black hole represents a self-sustained leaky Bose-condensate of N soft gravitons. In this picture the Hawking radiation is the quantum depletion of the condensate. Within this picture we show that quantum black hole physics is fully compatible with continuous global symmetries and that global hair appears with the strength B/N, where B is the global charge swallowed by the black hole. For large charge this hair has dramatic effect on black hole dynamics. Our findings can have interesting astrophysical consequences, such as existence of black holes with large detectable baryonic and leptonic numbers.

  10. Shaping Globular Clusters with Black Holes

    Science.gov (United States)

    Kohler, Susanna

    2018-03-01

    How many black holes lurk within the dense environments of globular clusters, and how do these powerful objects shape the properties of the cluster around them? One such cluster, NGC 3201, is now helping us to answer these questions.Hunting Stellar-Mass Black HolesSince the detection of merging black-hole binaries by the Laser Interferometer Gravitational-Wave Observatory (LIGO), the dense environments of globular clusters have received increasing attention as potential birthplaces of these compact binary systems.The central region of the globular star cluster NGC 3201, as viewed by Hubble. The black hole is in orbit with the star marked by the blue circle. [NASA/ESA]In addition, more and more stellar-mass black-hole candidates have been observed within globular clusters, lurking in binary pairs with luminous, non-compact companions. The most recent of these detections, found in the globular cluster NGC 3201, stands alone as the first stellar-mass black hole candidate discovered via radial velocity observations: the black holes main-sequence companion gave away its presence via a telltale wobble.Now a team of scientists led by Kyle Kremer (CIERA and Northwestern University) is using models of this system to better understand the impact that black holes might have on their host clusters.A Model ClusterThe relationship between black holes and their host clusters is complicated. Though the cluster environment can determine the dynamical evolution of the black holes, the retention rate of black holes in a globular cluster (i.e., how many remain in the cluster when they are born as supernovae, rather than being kicked out during the explosion) influences how the host cluster evolves.Kremer and collaborators track this complex relationship by modeling the evolution of a cluster similar to NGC 3201 with a Monte Carlo code. The code incorporates physics relevant to the evolution of black holes and black-hole binaries in globular clusters, such as two-body relaxation

  11. Stability of squashed Kaluza-Klein black holes

    International Nuclear Information System (INIS)

    Kimura, Masashi; Ishihara, Hideki; Murata, Keiju; Soda, Jiro

    2008-01-01

    The stability of squashed Kaluza-Klein black holes is studied. The squashed Kaluza-Klein black hole looks like a five-dimensional black hole in the vicinity of horizon and looks like a four-dimensional Minkowski spacetime with a circle at infinity. In this sense, squashed Kaluza-Klein black holes can be regarded as black holes in the Kaluza-Klein spacetimes. Using the symmetry of squashed Kaluza-Klein black holes, SU(2)xU(1)≅U(2), we obtain master equations for a part of the metric perturbations relevant to the stability. The analysis based on the master equations gives strong evidence for the stability of squashed Kaluza-Klein black holes. Hence, the squashed Kaluza-Klein black holes deserve to be taken seriously as realistic black holes in the Kaluza-Klein spacetime.

  12. Replication of alfalfa mosaic virus RNA 3 with movement and coat protein genes replaced by corresponding genes of Prunus necrotic ringspot ilarvirus.

    Science.gov (United States)

    Sánchez-Navarro, J A; Reusken, C B; Bol, J F; Pallás, V

    1997-12-01

    Alfalfa mosaic virus (AMV) and Prunus necrotic ringspot virus (PNRSV) are tripartite positive-strand RNA plant viruses that encode functionally similar translation products. Although the two viruses are phylogenetically closely related, they infect a very different range of natural hosts. The coat protein (CP) gene, the movement protein (MP) gene or both genes in AMV RNA 3 were replaced by the corresponding genes of PNRSV. The chimeric viruses were tested for heterologous encapsidation, replication in protoplasts from plants transformed with AMV replicase genes P1 and P2 (P12 plants) and for cell-to-cell transport in P12 plants. The chimeric viruses exhibited basic competence for encapsidation and replication in P12 protoplasts and for a low level of cell-to-cell movement in P12 plants. The potential involvement of the MP gene in determining host specificity in ilarviruses is discussed.

  13. Queering Black Racial Identity Development

    Science.gov (United States)

    Johnson, Alandis A.; Quaye, Stephen John

    2017-01-01

    We used queer theory to encourage readers to think differently about previous theories about Black racial identity development. Queer theory facilitates new and deeper understandings of how Black people develop their racial identities, prompting more fluidity and nuance. Specifically, we present a queered model of Black racial identity development…

  14. A Typology of Black Leadership.

    Science.gov (United States)

    Tryman, Donald L.

    1977-01-01

    A leadership typology is developed which describes the relationship of Black leadership styles to the Black masses. The role that such leadership plays in the political integration of Blacks as an ethnic group is analyzed. Four authority types are discussed: traditional brokerage, cultural nationalist, religious nationalist, and faddist…

  15. Black p-branes versus black holes in non-asymptotically flat Einstein-Yang-Mills theory

    Science.gov (United States)

    Habib Mazharimousavi, S.; Halilsoy, M.

    2016-09-01

    We present a class of non-asymptotically flat (NAF) charged black p-branes (BpB) with p-compact dimensions in higher-dimensional Einstein-Yang-Mills theory. Asymptotically the NAF structure manifests itself as an anti-de sitter spacetime. We determine the total mass/energy enclosed in a thin shell located outside the event horizon. By comparing the entropies of BpB with those of black holes in the same dimensions we derive transition criteria between the two types of black objects. Given certain conditions satisfied, our analysis shows that BpB can be considered excited states of black holes. An event horizon r+ versus charge square Q2 plot for the BpB reveals such a transition where r+ is related to the horizon radius rh of the black hole (BH) both with the common charge Q.

  16. The search for black holes

    International Nuclear Information System (INIS)

    Torn, K.

    1976-01-01

    Conceivable experimental investigations to prove the existence of black holes are discussed. Double system with a black hole turning around a star-satellite are in the spotlight. X-radiation emmited by such systems and resulting from accretion of the stellar gas by a black hole, and the gas heating when falling on the black hole might prove the model suggested. A source of strong X-radiation observed in the Cygnus star cluster and referred to as Cygnus X-1 may be thus identified as a black hole. Direct registration of short X-ray pulses with msec intervals might prove the suggestion. The lack of appropriate astrophysic facilities is pointed out to be the major difficulty on the way of experimental verifications

  17. Nonextremal stringy black hole

    International Nuclear Information System (INIS)

    Suzuki, K.

    1997-01-01

    We construct a four-dimensional BPS saturated heterotic string solution from the Taub-NUT solution. It is a nonextremal black hole solution since its Euler number is nonzero. We evaluate its black hole entropy semiclassically. We discuss the relation between the black hole entropy and the degeneracy of string states. The entropy of our string solution can be understood as the microscopic entropy which counts the elementary string states without any complications. copyright 1997 The American Physical Society

  18. How Black women make sense of 'White' and 'Black' fashion magazines: a qualitative think aloud study.

    Science.gov (United States)

    Ogden, Jane; Russell, Sheriden

    2013-12-01

    This qualitative think aloud study explored how Black women (n = 32) processed information from a White or Black fashion magazine. Comments to the 'White' magazine were characterised by rejection, being critical of the media and ambivalence, whereas they responded to the 'Black' magazine with celebration, identification and a search for depth. Transcending these themes was their self-identity of being a Black woman that was brought to the fore either by a sense of exclusion (White magazine) or engagement (Black magazine). Such an identity provides resilience against the media's thin ideals by minimising the processes of social comparison and internalisation.

  19. Charged black rings at large D

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Bin [Department of Physics and State Key Laboratory of Nuclear Physics and Technology,Peking University,5 Yiheyuan Rd, Beijing 100871 (China); Collaborative Innovation Center of Quantum Matter,5 Yiheyuan Rd, Beijing 100871 (China); Center for High Energy Physics, Peking University,5 Yiheyuan Rd, Beijing 100871 (China); Li, Peng-Cheng; Wang, Zi-zhi [Department of Physics and State Key Laboratory of Nuclear Physics and Technology,Peking University,5 Yiheyuan Rd, Beijing 100871 (China)

    2017-04-28

    We study the charged slowly rotating black holes in the Einstein-Maxwell theory in the large dimensions (D). By using the 1/D expansion in the near regions of the black holes we obtain the effective equations for the charged slowly rotating black holes. The effective equations capture the dynamics of various stationary solutions, including the charged black ring, the charged slowly rotating Myers-Perry black hole and the charged slowly boosted black string. Via different embeddings we construct these stationary solutions explicitly. For the charged black ring at large D, we find that the charge lowers the angular momentum due to the regularity condition on the solution. By performing the perturbation analysis of the effective equations, we obtain the quasinormal modes of the charge perturbation and the gravitational perturbation analytically. Like the neutral case the charged thin black ring suffers from the Gregory-Laflamme-like instability under the non-axisymmetric perturbations, but the charge weakens the instability. Besides, we find that the large D analysis always respects the cosmic censorship.

  20. VIOLENT BLACK MAN AND THE MYTH OF BLACK MACHO IN EUGENE O’NEILL’S "THE DREAMY KID"

    Directory of Open Access Journals (Sweden)

    Agnieszka Łobodziec

    2014-10-01

    Full Text Available The aim of this article is the investigation of the extent to which Eugene O’Neill, in his drama "The Dreamy Kid", (reconstructs, or employs, the black macho myth, which negatively images black men as marginalized and violent. This undertaking questions O’Neill’s self-proclaimed progressive racial attitudes and stated focus on the universal nature of human existence, which he professed to express through drama. Moreover, the article challenges the mainstream view of "The Dreamy Kid" as progressive. Although the play focalizes the experience of a marginalized black man, which could be interpreted as the playwright’s concern with the unjust racial and class stratification of American society, O’Neill’s production, unfortunately, contains a number of pervasive stereotypes relative to black people’s alleged moral degeneracy, primitiveness, and violent behavior, which threatens the white dominated constructed order. In terms of gendered racial politics, in the article attention is given to O’Neill’s presentation of black male negotiations with the white patriarchal power structure, embodied by the police forces, and with black matriarchy, represented by O’Neill’s stereotypical character portrayal of a dominant elderly black woman. Lastly, an overall analysis is performed in light of the call for an autonomous black art, which emerged among black artists in opposition to the demeaning, unrealistic, stereotypical portrayals of black victims of oppression by white American writers.

  1. Charged black holes with scalar hair

    Energy Technology Data Exchange (ETDEWEB)

    Fan, Zhong-Ying; Lü, H. [Center for Advanced Quantum Studies, Department of Physics,Beijing Normal University, Beijing 100875 (China)

    2015-09-10

    We consider a class of Einstein-Maxwell-Dilaton theories, in which the dilaton coupling to the Maxwell field is not the usual single exponential function, but one with a stationary point. The theories admit two charged black holes: one is the Reissner-Nordstrøm (RN) black hole and the other has a varying dilaton. For a given charge, the new black hole in the extremal limit has the same AdS{sub 2}×Sphere near-horizon geometry as the RN black hole, but it carries larger mass. We then introduce some scalar potentials and obtain exact charged AdS black holes. We also generalize the results to black p-branes with scalar hair.

  2. Information Retention by Stringy Black Holes

    CERN Document Server

    Ellis, John

    2015-01-01

    Building upon our previous work on two-dimensional stringy black holes and its extension to spherically-symmetric four-dimensional stringy black holes, we show how the latter retain information. A key r\\^ole is played by an infinite-dimensional $W_\\infty$ symmetry that preserves the area of an isolated black-hole horizon and hence its entropy. The exactly-marginal conformal world-sheet operator representing a massless stringy particle interacting with the black hole necessarily includes a contribution from $W_\\infty$ generators in its vertex function. This admixture manifests the transfer of information between the string black hole and external particles. We discuss different manifestations of $W_\\infty$ symmetry in black-hole physics and the connections between them.

  3. Is there life inside black holes?

    International Nuclear Information System (INIS)

    Dokuchaev, V I

    2011-01-01

    Bound inside rotating or charged black holes, there are stable periodic planetary orbits, which neither come out nor terminate at the central singularity. Stable periodic orbits inside black holes exist even for photons. These bound orbits may be defined as orbits of the third kind, following the Chandrasekhar classification of particle orbits in the black hole gravitational field. The existence domain for the third-kind orbits is rather spacious, and thus there is place for life inside supermassive black holes in the galactic nuclei. Interiors of the supermassive black holes may be inhabited by civilizations, being invisible from the outside. In principle, one can get information from the interiors of black holes by observing their white hole counterparts. (paper)

  4. Black holes turn white fast, otherwise stay black: no half measures

    International Nuclear Information System (INIS)

    Barceló, Carlos; Carballo-Rubio, Raúl; Garay, Luis J.

    2016-01-01

    Recently, various authors have proposed that the dominant ultraviolet effect in the gravitational collapse of massive stars to black holes is the transition between a black-hole geometry and a white-hole geometry, though their proposals are radically different in terms of their physical interpretation and characteristic time scales http://dx.doi.org/10.1142/S021827181442022X, http://dx.doi.org/10.1103/PhysRevD.92.104020. Several decades ago, it was shown by Eardley that white holes are highly unstable to the accretion of small amounts of matter, being rapidly turned into black holes http://dx.doi.org/10.1103/PhysRevLett.33.442. Studying the crossing of null shells on geometries describing the black-hole to white-hole transition, we obtain the conditions for the instability to develop in terms of the parameters of these geometries. We conclude that transitions with long characteristic time scales are pathologically unstable: occasional perturbations away from the perfect vacuum around these compact objects, even if being imperceptibly small, suffocate the white-hole explosion. On the other hand, geometries with short characteristic time scales are shown to be robust against perturbations, so that the corresponding processes could take place in real astrophysical scenarios. This motivates a conjecture about the transition amplitudes of different decay channels for black holes in a suitable ultraviolet completion of general relativity.

  5. Black holes and quantum processes in them

    International Nuclear Information System (INIS)

    Frolov, V.P.

    1976-01-01

    The latest achievements in the physics of black holes are reviewed. The problem of quantum production in a strong gravitational field of black holes is considered. Another parallel discovered during investigation of interactions between black holes and between black holes and surrounding media, is also drawn with thermodynamics. A gravitational field of rotating black holes is considered. Some cosmological aspects of evaporation of small black holes are discussed as well as possibilities to observe them

  6. The Black Man in American Society.

    Science.gov (United States)

    Framingham Public Schools, MA.

    GRADE OR AGES: Junior high school. SUBJECT MATTER: The black man in American society. ORGANIZATION AND PHYSICAL APPEARANCE: There are four major parts each with an overview. The four parts concern a) the African heritage of the black man, b) the American exploitation of the black man, c) the black man's contribution to American society, d) the…

  7. The Black Journalist in South Africa.

    Science.gov (United States)

    Hachten, William A.

    The role of the black journalist in South Africa is contradictory, precarious, and permeated with politics. There is little freedom of expression for blacks in South Africa, yet white-owned newspapers have expanded their coverage of black news, and some have special editions for black readers. As a result, the English language press is using more…

  8. Statistical clustering of primordial black holes

    Energy Technology Data Exchange (ETDEWEB)

    Carr, B J [Cambridge Univ. (UK). Inst. of Astronomy

    1977-04-01

    It is shown that Meszaros theory of galaxy formation, in which galaxies form from the density perturbations associated with the statistical fluctuation in the number density of primordial black holes, must be modified if the black holes are initially surrounded by regions of lower radiation density than average (as is most likely). However, even in this situation, the sort of effect Meszaros envisages does occur and could in principle cause galactic mass-scales to bind at the conventional time. In fact, the requirement that galaxies should not form prematurely implies that black holes could not have a critical density in the mass range above 10/sup 5/ M(sun). If the mass spectrum of primordial black holes falls off more slowly than m/sup -3/ (as expected), then the biggest black holes have the largest clustering effect. In this case the black hole clustering theory of galaxy formation reduces to the black hole seed theory of galaxy formation, in which each galaxy becomes bound under the gravitational influence of a single black hole nucleus. The seed theory could be viable only if the early Universe had a soft equation of state until a time exceeding 10/sup -4/ s or if something prevented black hole formation before 1 s.

  9. Black hole as a wormhole factory

    Directory of Open Access Journals (Sweden)

    Sung-Won Kim

    2015-12-01

    Full Text Available There have been lots of debates about the final fate of an evaporating black hole and the singularity hidden by an event horizon in quantum gravity. However, on general grounds, one may argue that a black hole stops radiation at the Planck mass (ħc/G1/2∼10−5 g, where the radiated energy is comparable to the black hole's mass. And also, it has been argued that there would be a wormhole-like structure, known as “spacetime foam”, due to large fluctuations below the Planck length (ħG/c31/2∼10−33 cm. In this paper, as an explicit example, we consider an exact classical solution which represents nicely those two properties in a recently proposed quantum gravity model based on different scaling dimensions between space and time coordinates. The solution, called “Black Wormhole”, consists of two different states, depending on its mass parameter M and an IR parameter ω: For the black hole state (with ωM2>1/2, a non-traversable wormhole occupies the interior region of the black hole around the singularity at the origin, whereas for the wormhole state (with ωM2<1/2, the interior wormhole is exposed to an outside observer as the black hole horizon is disappearing from evaporation. The black hole state becomes thermodynamically stable as it approaches the merging point where the interior wormhole throat and the black hole horizon merges, and the Hawking temperature vanishes at the exact merge point (with ωM2=1/2. This solution suggests the “Generalized Cosmic Censorship” by the existence of a wormhole-like structure which protects the naked singularity even after the black hole evaporation. One could understand the would-be wormhole inside the black hole horizon as the result of microscopic wormholes created by “negative” energy quanta which have entered the black hole horizon in Hawking radiation process; the quantum black hole could be a wormhole factory! It is found that this speculative picture may be consistent with the

  10. Black string in dRGT massive gravity

    Energy Technology Data Exchange (ETDEWEB)

    Tannukij, Lunchakorn [Mahidol University, Department of Physics, Faculty of Science, Bangkok (Thailand); Hanyang University, Department of Physics, Seoul (Korea, Republic of); Naresuan University, The Institute for Fundamental Study, Phitsanulok (Thailand); Wongjun, Pitayuth [Naresuan University, The Institute for Fundamental Study, Phitsanulok (Thailand); Ministry of Education, Thailand Center of Excellence in Physics, Bangkok (Thailand); Ghosh, Suchant G. [Jamia Millia Islamia, Centre of Theoretical Physics, New Delhi (India); University of Kwazulu-Natal, Astrophysics and Cosmology Research Unit, School of Mathematical Sciences, Durban (South Africa)

    2017-12-15

    We present a cylindrically symmetric solution, both charged and uncharged, which is known as a black string solution to the nonlinear ghost-free massive gravity found by de Rham, Gabadadze, and Tolley (dRGT). This ''dRGT black string'' can be thought of as a generalization of the black string solution found by Lemos. Moreover, the dRGT black string solution includes other classes of black string solution such as the monopole-black string ones since the graviton mass contributes to the global monopole term as well as the cosmological-constant term. To investigate the solution, we compute mass, temperature, and entropy of the dRGT black string. We found that the existence of the graviton mass drastically affects the thermodynamics of the black string. Furthermore, the Hawking-Page phase transition is found to be possible for the dRGT black string as well as the charged dRGT black string. The dRGT black string solution is thermodynamically stable for r > r{sub c} with negative thermodynamical potential and positive heat capacity while it is unstable for r < r{sub c} where the potential is positive. (orig.)

  11. Interior structure of rotating black holes. III. Charged black holes

    International Nuclear Information System (INIS)

    Hamilton, Andrew J. S.

    2011-01-01

    This paper extends to the case of charged rotating black holes the conformally stationary, axisymmetric, conformally separable solutions presented for uncharged rotating black holes in a companion paper. In the present paper, the collisionless fluid accreted by the black hole may be charged. The charge of the black hole is determined self-consistently by the charge accretion rate. As in the uncharged case, hyper-relativistic counterstreaming between ingoing and outgoing streams drives inflation at (just above) the inner horizon, followed by collapse. If both ingoing and outgoing streams are charged, then conformal separability holds during early inflation, but fails as inflation develops. If conformal separability is imposed throughout inflation and collapse, then only one of the ingoing and outgoing streams can be charged: the other must be neutral. Conformal separability prescribes a hierarchy of boundary conditions on the ingoing and outgoing streams incident on the inner horizon. The dominant radial boundary conditions require that the incident ingoing and outgoing number densities be uniform with latitude, but the charge per particle must vary with latitude such that the incident charge densities vary in proportion to the radial electric field. The subdominant angular boundary conditions require specific forms of the incident number- and charge-weighted angular motions. If the streams fall freely from outside the horizon, then the prescribed angular conditions can be achieved by the charged stream, but not by the neutral stream. Thus, as in the case of an uncharged black hole, the neutral stream must be considered to be delivered ad hoc to just above the inner horizon.

  12. Black hole shadow in an asymptotically flat, stationary, and axisymmetric spacetime: The Kerr-Newman and rotating regular black holes

    Science.gov (United States)

    Tsukamoto, Naoki

    2018-03-01

    The shadow of a black hole can be one of the strong observational evidences for stationary black holes. If we see shadows at the center of galaxies, we would say whether the observed compact objects are black holes. In this paper, we consider a formula for the contour of a shadow in an asymptotically-flat, stationary, and axisymmetric black hole spacetime. We show that the formula is useful for obtaining the contour of the shadow of several black holes such as the Kerr-Newman black hole and rotating regular black holes. Using the formula, we can obtain new examples of the contour of the shadow of rotating black holes if assumptions are satisfied.

  13. Turbulent black holes.

    Science.gov (United States)

    Yang, Huan; Zimmerman, Aaron; Lehner, Luis

    2015-02-27

    We demonstrate that rapidly spinning black holes can display a new type of nonlinear parametric instability-which is triggered above a certain perturbation amplitude threshold-akin to the onset of turbulence, with possibly observable consequences. This instability transfers from higher temporal and azimuthal spatial frequencies to lower frequencies-a phenomenon reminiscent of the inverse cascade displayed by (2+1)-dimensional fluids. Our finding provides evidence for the onset of transitory turbulence in astrophysical black holes and predicts observable signatures in black hole binaries with high spins. Furthermore, it gives a gravitational description of this behavior which, through the fluid-gravity duality, can potentially shed new light on the remarkable phenomena of turbulence in fluids.

  14. The renaissance of black phosphorus.

    Science.gov (United States)

    Ling, Xi; Wang, Han; Huang, Shengxi; Xia, Fengnian; Dresselhaus, Mildred S

    2015-04-14

    One hundred years after its first successful synthesis in the bulk form in 1914, black phosphorus (black P) was recently rediscovered from the perspective of a 2D layered material, attracting tremendous interest from condensed matter physicists, chemists, semiconductor device engineers, and material scientists. Similar to graphite and transition metal dichalcogenides (TMDs), black P has a layered structure but with a unique puckered single-layer geometry. Because the direct electronic band gap of thin film black P can be varied from 0.3 eV to around 2 eV, depending on its film thickness, and because of its high carrier mobility and anisotropic in-plane properties, black P is promising for novel applications in nanoelectronics and nanophotonics different from graphene and TMDs. Black P as a nanomaterial has already attracted much attention from researchers within the past year. Here, we offer our opinions on this emerging material with the goal of motivating and inspiring fellow researchers in the 2D materials community and the broad readership of PNAS to discuss and contribute to this exciting new field. We also give our perspectives on future 2D and thin film black P research directions, aiming to assist researchers coming from a variety of disciplines who are desirous of working in this exciting research field.

  15. Australian black coal statistics 1990

    Energy Technology Data Exchange (ETDEWEB)

    1991-01-01

    This second edition of Australian black coal statistics replaces the Joint Coal Board's publication 'Black coal in Australia'. It includes an expanded international coal trade supplement. Sections cover resources of black coal, coal supply and demand, coal production, employment and productivity of mines, export data, coal consumption and a directory of producers.

  16. Spin One Hawking Radiation from Dirty Black Holes

    OpenAIRE

    Petarpa Boonserm; Tritos Ngampitipan; Matt Visser

    2013-01-01

    A “clean” black hole is a black hole in vacuum such as the Schwarzschild black hole. However in real physical systems, there are matter fields around a black hole. Such a black hole is called a “dirty black hole”. In this paper, the effect of matter fields on the black hole and the greybody factor is investigated. The results show that matter fields make a black hole smaller. They can increase the potential energy to a black hole to obstruct Hawking radiation to propagate. This causes the gre...

  17. Oxidative stress induction by Prunus necrotic ringspot virus infection in apricot seeds.

    Science.gov (United States)

    Amari, Khalid; Díaz-Vivancos, Pedro; Pallás, Vincente; Sánchez-Pina, María Amelia; Hernández, José Antonio

    2007-10-01

    Prunus necrotic ringspot rvirus (PNRSV) was able to invade the immature apricot seed including the embryo. The amount of virus was very high inside the embryo compared with that present in the cotyledons. PNRSV infection produced an oxidative stress in apricot seeds as indicated by the increase in lipid peroxidation, measured as thiobarbituric acid-reactive substances. This lipid peroxidation increase was parallelled with an imbalance in the seed antioxidant enzymes. A significant decrease in the ascorbate-GSH cycle enzymes as well as in peroxidase (POX) activity took place in infected seeds, suggesting a low capability to eliminate H2O2. No changes in superoxide dismutase (SOD) or catalase activity were observed. A significant decrease in polyphenoloxidase (PPO) activity was also observed. Native PAGE revealed the presence of three different SOD activity bands in apricot seeds: a Mn-containing SOD and two CuZn-containing SODs. Only an isozyme with catalase, glutathione reductase (GR) or PPO activity was detected in both healthy and infected apricot seeds. Regarding POX staining, three bands with POX activity were detected in native gels in both healthy and infected seeds. The gel results emphasise that the drop detected in POX, GR and PPO activities in PNRSV-infected apricot seeds by kinetic analyses was also evident from the results obtained by native PAGE. The oxidative stress and the imbalance in the antioxidant systems from PNRSV-infected apricot seeds resemble the hypersensitive response observed in some virus-host interactions. This defence mechanism would inactivate PNRSV during seed formation and/or the storage period or even during seed germination. Those results can explain the decrease in seed germination and the low transmission of PNRSV by seeds in apricot trees.

  18. Black holes and everyday physics

    International Nuclear Information System (INIS)

    Bekenstein, J.D.

    1982-01-01

    Black holes have piqued much curiosity. But thus far they have been important only in ''remote'' subjects like astrophysics and quantum gravity. It is shown that the situation can be improved. By a judicious application of black hole physics, one can obtain new results in ''everyday physics''. For example, black holes yield a quantum universal upper bound on the entropy-to-energy ratio for ordinary thermodynamical systems which was unknown earlier. It can be checked, albeit with much labor, by ordinary statistical methods. Black holes set a limitation on the number of species of elementary particles-quarks, leptons, neutrinos - which may exist. And black holes lead to a fundamental limitation on the rate at which information can be transferred for given message energy by any communication system. (author)

  19. Black hole final state conspiracies

    International Nuclear Information System (INIS)

    McInnes, Brett

    2009-01-01

    The principle that unitarity must be preserved in all processes, no matter how exotic, has led to deep insights into boundary conditions in cosmology and black hole theory. In the case of black hole evaporation, Horowitz and Maldacena were led to propose that unitarity preservation can be understood in terms of a restriction imposed on the wave function at the singularity. Gottesman and Preskill showed that this natural idea only works if one postulates the presence of 'conspiracies' between systems just inside the event horizon and states at much later times, near the singularity. We argue that some AdS black holes have unusual internal thermodynamics, and that this may permit the required 'conspiracies' if real black holes are described by some kind of sum over all AdS black holes having the same entropy

  20. Gamma ray bursts of black hole universe

    Science.gov (United States)

    Zhang, T. X.

    2015-07-01

    Slightly modifying the standard big bang theory, Zhang recently developed a new cosmological model called black hole universe, which has only a single postulate but is consistent with Mach's principle, governed by Einstein's general theory of relativity, and able to explain existing observations of the universe. In the previous studies, we have explained the origin, structure, evolution, expansion, cosmic microwave background radiation, quasar, and acceleration of black hole universe, which grew from a star-like black hole with several solar masses through a supermassive black hole with billions of solar masses to the present state with hundred billion-trillions of solar masses by accreting ambient matter and merging with other black holes. This study investigates gamma ray bursts of black hole universe and provides an alternative explanation for the energy and spectrum measurements of gamma ray bursts according to the black hole universe model. The results indicate that gamma ray bursts can be understood as emissions of dynamic star-like black holes. A black hole, when it accretes its star or merges with another black hole, becomes dynamic. A dynamic black hole has a broken event horizon and thus cannot hold the inside hot (or high-frequency) blackbody radiation, which flows or leaks out and produces a GRB. A star when it collapses into its core black hole produces a long GRB and releases the gravitational potential energy of the star as gamma rays. A black hole that merges with another black hole produces a short GRB and releases a part of their blackbody radiation as gamma rays. The amount of energy obtained from the emissions of dynamic star-like black holes are consistent with the measurements of energy from GRBs. The GRB energy spectra derived from this new emission mechanism are also consistent with the measurements.

  1. Black holes matter

    DEFF Research Database (Denmark)

    Kragh, Helge Stjernholm

    2016-01-01

    Review essay, Marcia Bartusiak, Black Hole: How an Idea Abandoned by Newtonians, Hated by Einstein, and Gambled On by Hawking Became Loved (New Haven: Yale University Press, 2015).......Review essay, Marcia Bartusiak, Black Hole: How an Idea Abandoned by Newtonians, Hated by Einstein, and Gambled On by Hawking Became Loved (New Haven: Yale University Press, 2015)....

  2. "Styled by Their Perceptions": Black Adolescent Girls Interpret Representations of Black Females in Popular Culture

    Science.gov (United States)

    Muhammad, Gholnecsar E.; McArthur, Sherell A.

    2015-01-01

    Identity formation is a critical process shaping the lives of adolescents and can present distinct challenges for Black adolescent girls who are positioned in society to negotiate ideals of self when presented with false and incomplete images representing Black girlhood. Researchers have found distorted images of Black femininity derived from…

  3. The black church as the womb of black liberation theology?: Why the ...

    African Journals Online (AJOL)

    2015-04-30

    Apr 30, 2015 ... Southern Africa (URCSA) is not a genuine black church? This article ..... peculiar constituency of black slaves and freed men[sic] what no one else was .... church in the USA, which started during the early 20th century when ...

  4. Chemical characterization and thermal properties of kernel oils from Tunisian peach and nectarine varieties of Prunus persica; Caracterización química y propiedades térmicas de los aceites de semillas de variedades tunecinas de melocotón y nectarina de Prunus pérsica

    Energy Technology Data Exchange (ETDEWEB)

    Chamli, D.; Bootello, M.A.; Bouali, I.; Jouhri, S.; Boukhchina, S.; Martínez-Force, S.

    2017-07-01

    A comparative study was conducted to determine the fatty acids, triacylglycerol compositions and thermal properties of Tunisian kernel oils from the Prunus persica varieties, peach and nectarine, grown in two areas of Tunisia, Gabes and Morneg. Qualitatively, the fatty acids composition and triacylglycerol species were identical for all samples. Oleic acid (67.7-75.0%) was the main fatty acid, followed by linoleic (15.7-22.1%) and palmitic (5.6-6.3%) acids. The major triacylglycerol species were triolein, OOO (38.4-50.5%), followed by OOL (18.2-23.2%), POO (8.3-9.7%) and OLL (6.3-10.1%). The thermal profiles were highly influenced by the high content of triolein due to the importance of oleic acid in these oils. Moreover, the fatty acids distribution in TAG external positions was determined as corresponding to an α asymmetry coefficient that was between 0.10 and 0.12, indicating a high asymmetry in the distribution of saturated fatty acids in the position sn-1 and sn-3 in the TAG species of all samples. [Spanish] Se ha realizado un estudio comparativo de aceites tunecinos obtenidos a partir de las semillas de variedades de Prunus persica, melocotón y nectarina, cultivadas en dos zonas de Túnez, Gabes y Morneg. Cualitativamente, la composición de ácidos grasos y de especies de triglicéridos fueron idénticas para todas las muestras. El ácido oleico (67,7-75,0%) fue el ácido graso principal, seguido del linoleico (15,7-22,1%) y el palmítico (5,6-6,3%). Las especies principales de triacilglicéridos fueron la trioleina, OOO (38,4-50,5%), seguida de OOL (18,2-23,2%), POO (8,3-9,7%) y OLL (6,3-10,1%). Los perfiles térmicos fueron muy influidos por el alto contenido de trioleina debido a la importancia del ácido oleico en estos aceites. Por otra parte, se determinó la distribución de ácidos grasos en las posiciones externas de los TAG correspondiendo a un coeficiente de asimetría α entre 0,10 y 0,12, lo que indica una alta asimetría en la distribuci

  5. Overview of prostate cancer in indigenous black Africans and blacks of African ancestry in diaspora 1935-2007.

    Science.gov (United States)

    Magoha, G A O

    2007-09-01

    To carry out an overview of prostate cancer in indigenous back Africans in sub-Saharan Africa and blacks of African ancestry in diaspora. Review of all published literature on prostate cancer on indigenous black Africans and Africans in diaspora was carried out through medline and index medicus searches. Published data of prostate cancer in indigenous black Africans and black men in diaspora from 1935-2007 were included in the review. Abstracts of articles identified were assessed, read and analysed to determine their possible suitability and relevance to the title under review. After establishing relevance from the abstract, the entire paper was read, and the significant points included in the review. Prostate cancer incidence and magnitude in black Africans was grossly misunderstood and underestimated in the past. Prostate cancer incidence is on the increase and currently is perhaps the most common urological malignancy affecting black Africans. Its incidence and clinical characteristics is similar to that of the Africans in diaspora but different from all other races. There currently exists significant evidence which suggests a common enhancing genetic predisposition in black men to prostate cancer. There is very urgent need for further investigation of this phenomenon through randomised controlled multicentre studies involving indigenous black Africans and black men in diaspora.

  6. Warped products and black holes

    International Nuclear Information System (INIS)

    Hong, Soon-Tae

    2005-01-01

    We apply the warped product space-time scheme to the Banados-Teitelboim-Zanelli black holes and the Reissner-Nordstroem-anti-de Sitter black hole to investigate their interior solutions in terms of warped products. It is shown that there exist no discontinuities of the Ricci and Einstein curvatures across event horizons of these black holes

  7. Uncovering Black Womanhood in Engineering

    Science.gov (United States)

    Gibson, Sheree L.; Espino, Michelle M.

    2016-01-01

    Despite the growing research that outlines the experiences of Blacks and women undergraduates in engineering, little is known about Black women in this field. The purpose of this qualitative study was to uncover how eight Black undergraduate women in engineering understood their race and gender identities in a culture that can be oppressive to…

  8. Instability of ultra-spinning black holes

    International Nuclear Information System (INIS)

    Emparan, Roberto; Myers, Robert C.

    2003-01-01

    It has long been known that, in higher-dimensional general relativity, there are black hole solutions with an arbitrarily large angular momentum for a fixed mass. We examine the geometry of the event horizon of such ultra-spinning black holes and argue that these solutions become unstable at large enough rotation. Hence we find that higher-dimensional general relativity imposes an effective 'Kerr-bound' on spinning black holes through a dynamical decay mechanism. Our results also give indications of the existence of new stationary black holes with 'rippled' horizons of spherical topology. We consider various scenarios for the possible decay of ultra-spinning black holes, and finally discuss the implications of our results for black holes in braneworld scenarios. (author)

  9. Braneworld black holes and entropy bounds

    Directory of Open Access Journals (Sweden)

    Y. Heydarzade

    2018-01-01

    Full Text Available The Bousso's D-bound entropy for the various possible black hole solutions on a 4-dimensional brane is checked. It is found that the D-bound entropy here is apparently different from that of obtained for the 4-dimensional black hole solutions. This difference is interpreted as the extra loss of information, associated to the extra dimension, when an extra-dimensional black hole is moved outward the observer's cosmological horizon. Also, it is discussed that N-bound entropy is hold for the possible solutions here. Finally, by adopting the recent Bohr-like approach to black hole quantum physics for the excited black holes, the obtained results are written also in terms of the black hole excited states.

  10. Atomic structure in black hole

    International Nuclear Information System (INIS)

    Nagatani, Yukinori

    2006-01-01

    We propose that any black hole has atomic structure in its inside and has no horizon as a model of black holes. Our proposal is founded on a mean field approximation of gravity. The structure of our model consists of a (charged) singularity at the center and quantum fluctuations of fields around the singularity, namely, it is quite similar to that of atoms. Any properties of black holes, e.g. entropy, can be explained by the model. The model naturally quantizes black holes. In particular, we find the minimum black hole, whose structure is similar to that of the hydrogen atom and whose Schwarzschild radius is approximately 1.1287 times the Planck length. Our approach is conceptually similar to Bohr's model of the atomic structure, and the concept of the minimum Schwarzschild radius is similar to that of the Bohr radius. The model predicts that black holes carry baryon number, and the baryon number is rapidly violated. This baryon number violation can be used as verification of the model. (author)

  11. Do David and Goliath Play the Same Game? Explanation of the Abundance of Rare and Frequent Invasive Alien Plants in Urban Woodlands in Warsaw, Poland.

    Science.gov (United States)

    Obidziński, Artur; Mędrzycki, Piotr; Kołaczkowska, Ewa; Ciurzycki, Wojciech; Marciszewska, Katarzyna

    2016-01-01

    Invasive Alien Plants occur in numbers differing by orders of magnitude at subsequent invasion stages. Effective sampling and quantifying niches of rare invasive plants are quite problematic. The aim of this paper is an estimation of the influence of invasive plants frequency on the explanation of their local abundance. We attempted to achieve it through: (1) assessment of occurrence of self-regenerating invasive plants in urban woodlands, (2) comparison of Random Forest modelling results for frequent and rare species. We hypothesized that the abundance of frequent species would be explained better than that of rare ones and that both rare and frequent species share a common hierarchy of the most important determinants. We found 15 taxa in almost two thirds of 1040 plots with a total number of 1068 occurrences. There were recorded 6 taxa of high frequency-Prunus serotina, Quercus rubra, Acer negundo, Robinia pseudoacacia, Impatiens parviflora and Solidago spp.-and 9 taxa of low frequency: Acer saccharinum, Amelanchier spicata, Cornus spp., Fraxinus spp., Parthenocissus spp., Syringa vulgaris, Echinocystis lobata, Helianthus tuberosus, Reynoutria spp. Random Forest's models' quality grows with the number of occurrences of frequent taxa but not of the rare ones. Both frequent and rare taxa share a similar hierarchy of predictors' importance: Land use > Tree stand > Seed source and, for frequent taxa, Forest properties as well. We conclude that there is an 'explanation jump' at higher species frequencies, but rare species are surprisingly similar to frequent ones in their determinant's hierarchy, with differences conforming with their respective stages of invasion.

  12. Do David and Goliath Play the Same Game? Explanation of the Abundance of Rare and Frequent Invasive Alien Plants in Urban Woodlands in Warsaw, Poland.

    Directory of Open Access Journals (Sweden)

    Artur Obidziński

    Full Text Available Invasive Alien Plants occur in numbers differing by orders of magnitude at subsequent invasion stages. Effective sampling and quantifying niches of rare invasive plants are quite problematic. The aim of this paper is an estimation of the influence of invasive plants frequency on the explanation of their local abundance. We attempted to achieve it through: (1 assessment of occurrence of self-regenerating invasive plants in urban woodlands, (2 comparison of Random Forest modelling results for frequent and rare species. We hypothesized that the abundance of frequent species would be explained better than that of rare ones and that both rare and frequent species share a common hierarchy of the most important determinants. We found 15 taxa in almost two thirds of 1040 plots with a total number of 1068 occurrences. There were recorded 6 taxa of high frequency-Prunus serotina, Quercus rubra, Acer negundo, Robinia pseudoacacia, Impatiens parviflora and Solidago spp.-and 9 taxa of low frequency: Acer saccharinum, Amelanchier spicata, Cornus spp., Fraxinus spp., Parthenocissus spp., Syringa vulgaris, Echinocystis lobata, Helianthus tuberosus, Reynoutria spp. Random Forest's models' quality grows with the number of occurrences of frequent taxa but not of the rare ones. Both frequent and rare taxa share a similar hierarchy of predictors' importance: Land use > Tree stand > Seed source and, for frequent taxa, Forest properties as well. We conclude that there is an 'explanation jump' at higher species frequencies, but rare species are surprisingly similar to frequent ones in their determinant's hierarchy, with differences conforming with their respective stages of invasion.

  13. Photosynthetic responses to understory shade and elevated carbon dioxide concentration in 4 northern hardwood tree species

    International Nuclear Information System (INIS)

    Sefcik, L.T.; Zak, D.R.; Ellsworth, D.S.

    2006-01-01

    Stimulation of photosynthesis in response to elevated carbon dioxide (CO 2 ) varies among tree species and species groups. In this study, seedling responses to elevated atmospheric carbon dioxide (CO 2 ) concentrations and solar irradiance over 2 growing seasons were investigated for shade tolerant Acer saccharum Marsh.; Fagus grandifolia J.F. Ehrh; and shade-intolerant Prunus serotina. Seedlings were exposed to a combination of elevated and ambient concentrations of CO 2 and understory shade in open-top chambers placed in a forest understory. It was observed that the elevated CO 2 treatment increased mean light-saturated net photosynthetic rates by 63 per cent in the shade-tolerant species and 67 per cent in the shade-intolerant species. When measured at the elevated CO 2 , long-term enhancement of photosynthesis was 10 per cent lower than the instantaneous enhancement observed in ambient-CO 2 -grown plants. As the growth irradiance increased, proportional enhancement due to elevated CO 2 decreased from 97 per cent for plants grown in deep shade to 47 per cent for plants grown in moderate shade. Results indicated that in nitrogen (N) limited northern temperate forests, trees grown in deep shade may display greater photosynthetic gains from a CO 2 enriched atmosphere than trees growing in more moderate shade, due to greater down-regulation. It was concluded that if elevated CO 2 levels promote the survival of shade-intolerant species in dim understory light, the future composition and dynamics of successional forest communities may be altered. 70 refs., 2 tabs., 3 figs

  14. Do David and Goliath Play the Same Game? Explanation of the Abundance of Rare and Frequent Invasive Alien Plants in Urban Woodlands in Warsaw, Poland

    Science.gov (United States)

    Mędrzycki, Piotr; Kołaczkowska, Ewa; Ciurzycki, Wojciech; Marciszewska, Katarzyna

    2016-01-01

    Invasive Alien Plants occur in numbers differing by orders of magnitude at subsequent invasion stages. Effective sampling and quantifying niches of rare invasive plants are quite problematic. The aim of this paper is an estimation of the influence of invasive plants frequency on the explanation of their local abundance. We attempted to achieve it through: (1) assessment of occurrence of self-regenerating invasive plants in urban woodlands, (2) comparison of Random Forest modelling results for frequent and rare species. We hypothesized that the abundance of frequent species would be explained better than that of rare ones and that both rare and frequent species share a common hierarchy of the most important determinants. We found 15 taxa in almost two thirds of 1040 plots with a total number of 1068 occurrences. There were recorded 6 taxa of high frequency–Prunus serotina, Quercus rubra, Acer negundo, Robinia pseudoacacia, Impatiens parviflora and Solidago spp.–and 9 taxa of low frequency: Acer saccharinum, Amelanchier spicata, Cornus spp., Fraxinus spp., Parthenocissus spp., Syringa vulgaris, Echinocystis lobata, Helianthus tuberosus, Reynoutria spp. Random Forest’s models’ quality grows with the number of occurrences of frequent taxa but not of the rare ones. Both frequent and rare taxa share a similar hierarchy of predictors’ importance: Land use > Tree stand > Seed source and, for frequent taxa, Forest properties as well. We conclude that there is an ‘explanation jump’ at higher species frequencies, but rare species are surprisingly similar to frequent ones in their determinant’s hierarchy, with differences conforming with their respective stages of invasion. PMID:27992516

  15. Quantum information erasure inside black holes

    International Nuclear Information System (INIS)

    Lowe, David A.; Thorlacius, Larus

    2015-01-01

    An effective field theory for infalling observers in the vicinity of a quasi-static black hole is given in terms of a freely falling lattice discretization. The lattice model successfully reproduces the thermal spectrum of outgoing Hawking radiation, as was shown by Corley and Jacobson, but can also be used to model observations made by a typical low-energy observer who enters the black hole in free fall at a prescribed time. The explicit short distance cutoff ensures that, from the viewpoint of the infalling observer, any quantum information that entered the black hole more than a scrambling time earlier has been erased by the black hole singularity. This property, combined with the requirement that outside observers need at least of order the scrambling time to extract quantum information from the black hole, ensures that a typical infalling observer does not encounter drama upon crossing the black hole horizon in a theory where black hole information is preserved for asymptotic observers.

  16. Watchable Wildlife: The Black Bear

    Science.gov (United States)

    Lynn L. Rogers

    1992-01-01

    Black bears are the bears people most often encounter. Black bears live in forests over much of North America, unlike grizzlies that live only in Alaska, northern and western Canada, and the northern Rocky Mountains. This brochure presents the latest information on black bear life and how this species responds to an ever-increasing number of campers, hikers, and...

  17. Black Thyroid Associated with Thyroid Carcinoma

    Directory of Open Access Journals (Sweden)

    Emad Kandil

    2010-01-01

    Full Text Available Objective. Black thyroid is a rare pigmented change seen almost exclusively in patients upon minocycline ingestion, and the process has previously been thought to be generally benign. There have been 61 reported cases of black thyroid. We are aware of 13 cases previously reported in association with thyroid carcinoma. This paper reports six patients with black thyroid pigmentation in association with thyroid carcinoma. Design. The medical records of six patients who were diagnosed with black thyroid syndrome, all of whom underwent thyroid surgery, were reviewed. Data on age, gender, race, preoperative fine needle aspiration biopsy (FNA, thyroid function levels, and pathology reports were collected. Main Outcome. The mean age was 60 years. There were 5 females, 4 of whom were African American. All patients were clinically and biochemically euthyroid. Black pigmentation was not diagnosed in preoperative FNA, and only one patient had a preoperative diagnosis of papillary thyroid carcinoma. The other patients underwent surgery and were found to have black pigmentation of the thyroid associated with carcinoma. Conclusions. FNA does not diagnose black thyroid, which is associated with thyroid carcinoma. Thyroid glands with black pigmentation deserve thorough pathologic examination, including several sections of each specimen.

  18. Black holes new horizons

    CERN Document Server

    Hayward, Sean Alan

    2013-01-01

    Black holes, once just fascinating theoretical predictions of how gravity warps space-time according to Einstein's theory, are now generally accepted as astrophysical realities, formed by post-supernova collapse, or as supermassive black holes mysteriously found at the cores of most galaxies, powering active galactic nuclei, the most powerful objects in the universe. Theoretical understanding has progressed in recent decades with a wider realization that local concepts should characterize black holes, rather than the global concepts found in textbooks. In particular, notions such as trapping h

  19. Geometry of higher-dimensional black hole thermodynamics

    International Nuclear Information System (INIS)

    Aaman, Jan E.; Pidokrajt, Narit

    2006-01-01

    We investigate thermodynamic curvatures of the Kerr and Reissner-Nordstroem (RN) black holes in spacetime dimensions higher than four. These black holes possess thermodynamic geometries similar to those in four-dimensional spacetime. The thermodynamic geometries are the Ruppeiner geometry and the conformally related Weinhold geometry. The Ruppeiner geometry for a d=5 Kerr black hole is curved and divergent in the extremal limit. For a d≥6 Kerr black hole there is no extremality but the Ruppeiner curvature diverges where one suspects that the black hole becomes unstable. The Weinhold geometry of the Kerr black hole in arbitrary dimension is a flat geometry. For the RN black hole the Ruppeiner geometry is flat in all spacetime dimensions, whereas its Weinhold geometry is curved. In d≥5 the Kerr black hole can possess more than one angular momentum. Finally we discuss the Ruppeiner geometry for the Kerr black hole in d=5 with double angular momenta

  20. Ain't I Black Too: Counterstories of Black Atheists in College

    Science.gov (United States)

    Snipes, Jeremy T.

    2017-01-01

    Increasingly Black college students are identifying as atheist, however few empirical studies in higher education and student affairs are exploring the phenomenon. This dissertation examines the question, "How do Black atheist understand their identity in college?" Using Higginbotham's Politics of Respectability and tenants of Critical…

  1. Statistical Hair on Black Holes

    International Nuclear Information System (INIS)

    Strominger, A.

    1996-01-01

    The Bekenstein-Hawking entropy for certain BPS-saturated black holes in string theory has recently been derived by counting internal black hole microstates at weak coupling. We argue that the black hole microstate can be measured by interference experiments even in the strong coupling region where there is clearly an event horizon. Extracting information which is naively behind the event horizon is possible due to the existence of statistical quantum hair carried by the black hole. This quantum hair arises from the arbitrarily large number of discrete gauge symmetries present in string theory. copyright 1996 The American Physical Society

  2. On black hole horizon fluctuations

    International Nuclear Information System (INIS)

    Tuchin, K.L.

    1999-01-01

    A study of the high angular momentum particles 'atmosphere' near the Schwarzschild black hole horizon suggested that strong gravitational interactions occur at invariant distance of the order of 3 √M [2]. We present a generalization of this result to the Kerr-Newman black hole case. It is shown that the larger charge and angular momentum black hole bears, the larger invariant distance at which strong gravitational interactions occur becomes. This invariant distance is of order 3 √((r + 2 )/((r + - r - ))). This implies that the Planckian structure of the Hawking radiation of extreme black holes is completely broken

  3. Thermodynamics of Accelerating Black Holes.

    Science.gov (United States)

    Appels, Michael; Gregory, Ruth; Kubizňák, David

    2016-09-23

    We address a long-standing problem of describing the thermodynamics of an accelerating black hole. We derive a standard first law of black hole thermodynamics, with the usual identification of entropy proportional to the area of the event horizon-even though the event horizon contains a conical singularity. This result not only extends the applicability of black hole thermodynamics to realms previously not anticipated, it also opens a possibility for studying novel properties of an important class of exact radiative solutions of Einstein equations describing accelerated objects. We discuss the thermodynamic volume, stability, and phase structure of these black holes.

  4. Black Political Socialization and Political Change: The Black Panther Party Platform as a "Model" of Radical Political Socialization

    Science.gov (United States)

    McLemore, Leslie Burl

    1975-01-01

    Considers the potential impact of black revolutionary nationalism--cultural and military--upon the social patterns of black people, arguing that a racial, cultural, and military approach through black organizations with a highly developed system of communications holds the key to black salvation in America. (Author/JM)

  5. Compressibility of rotating black holes

    International Nuclear Information System (INIS)

    Dolan, Brian P.

    2011-01-01

    Interpreting the cosmological constant as a pressure, whose thermodynamically conjugate variable is a volume, modifies the first law of black hole thermodynamics. Properties of the resulting thermodynamic volume are investigated: the compressibility and the speed of sound of the black hole are derived in the case of nonpositive cosmological constant. The adiabatic compressibility vanishes for a nonrotating black hole and is maximal in the extremal case--comparable with, but still less than, that of a cold neutron star. A speed of sound v s is associated with the adiabatic compressibility, which is equal to c for a nonrotating black hole and decreases as the angular momentum is increased. An extremal black hole has v s 2 =0.9 c 2 when the cosmological constant vanishes, and more generally v s is bounded below by c/√(2).

  6. Black-hole creation in quantum cosmology

    Energy Technology Data Exchange (ETDEWEB)

    Zhong Chao, Wu [Rome, Univ. `La Sapienza` (Italy). International Center for Relativistic Astrophysics]|[Specola Vaticana, Vatican City State (Vatican City State, Holy See)

    1997-11-01

    It is proven that the probability of a black hole created from the de Sitter space-time background, at the Wkb level, is the exponential of one quarter of the sum of the black hole and cosmological horizon areas, or the total entropy of the universe. This is true not only for the spherically symmetric cases of the Schwarzschild or Reissner-Nordstroem black holes, but also for the rotating cases of the Kerr black hole and the rotating charged case of the Newman black hole. The de Sitter metric is the most probable evolution at the Planckian era of the universe.

  7. Six-dimensional localized black holes: Numerical solutions

    International Nuclear Information System (INIS)

    Kudoh, Hideaki

    2004-01-01

    To test the strong-gravity regime in Randall-Sundrum braneworlds, we consider black holes bound to a brane. In a previous paper, we studied numerical solutions of localized black holes whose horizon radii are smaller than the AdS curvature radius. In this paper, we improve the numerical method and discuss properties of the six-dimensional (6D) localized black holes whose horizon radii are larger than the AdS curvature radius. At a horizon temperature T≅1/2πl, the thermodynamics of the localized black hole undergo a transition with its character changing from a 6D Schwarzschild black hole type to a 6D black string type. The specific heat of the localized black holes is negative, and the entropy is greater than or nearly equal to that of the 6D black strings with the same thermodynamic mass. The large localized black holes show flattened horizon geometries, and the intrinsic curvature of the horizon four-geometry becomes negative near the brane. Our results indicate that the recovery mechanism of lower-dimensional Einstein gravity on the brane works even in the presence of the black holes

  8. Black holes with halos

    Science.gov (United States)

    Monten, Ruben; Toldo, Chiara

    2018-02-01

    We present new AdS4 black hole solutions in N =2 gauged supergravity coupled to vector and hypermultiplets. We focus on a particular consistent truncation of M-theory on the homogeneous Sasaki–Einstein seven-manifold M 111, characterized by the presence of one Betti vector multiplet. We numerically construct static and spherically symmetric black holes with electric and magnetic charges, corresponding to M2 and M5 branes wrapping non-contractible cycles of the internal manifold. The novel feature characterizing these nonzero temperature configurations is the presence of a massive vector field halo. Moreover, we verify the first law of black hole mechanics and we study the thermodynamics in the canonical ensemble. We analyze the behavior of the massive vector field condensate across the small-large black hole phase transition and we interpret the process in the dual field theory.

  9. From Black Power to Black Studies: How a Radical Social Movement became an Academic Discipline

    Science.gov (United States)

    Rojas, Fabio

    2007-01-01

    The black power movement helped redefine African Americans' identity and establish a new racial consciousness in the 1960s. As an influential political force, this movement in turn spawned the academic discipline known as Black Studies. Today there are more than a hundred Black Studies degree programs in the United States, many of them located in…

  10. Post-storage cell wall metabolism in two sweet cherry (Prunus avium L.) cultivars displaying different postharvest performance.

    Science.gov (United States)

    Belge, Burcu; Comabella, Eva; Graell, Jordi; Lara, Isabel

    2015-09-01

    The biochemical processes underlying firmness loss of sweet cherry (Prunus avium L.) fruit are poorly understood. Studies on cell wall metabolism of sweet cherry have been generally undertaken during on-tree development or at harvest maturity, while published reports on postharvest changes are scarce and fragmentary. In this work, cell wall modifications after storage at 0 ℃ were studied in two cherry cultivars ('Celeste' and 'Somerset') displaying different postharvest potential. Firmness was largely determined by the yields of the Na2CO3- and KOH-soluble fractions, enriched in covalently-bound pectins and in matrix glycans, respectively, and correlated well with ascorbic acid contents. The yields of these two cell wall fractions were correlated inversely with pectinmethylesterase and endo-1,4-β-d-glucanase activities, indicating a relevant role of these two enzymes in postharvest firmness changes in sweet cherry. The amount of solubilised cell wall materials was closely associated to the contents of dehydroascorbic acid, suggesting the possible involvement of oxidative mechanisms in cell wall disassembly. These data may help understanding the evolution of fruit quality during the marketing period, and give hints for the design of suitable management strategies to preserve key attributes. © The Author(s) 2014.

  11. Preclinical renal chemo-protective potential of Prunus amygdalus Batsch seed coat via alteration of multiple molecular pathways.

    Science.gov (United States)

    Pandey, Preeti; Bhatt, Prakash Chandra; Rahman, Mahfoozur; Patel, Dinesh Kumar; Anwar, Firoz; Al-Abbasi, Fahad; Verma, Amita; Kumar, Vikas

    2018-02-01

    Prunus amygdalus Batsch (almond) is a classical nutritive traditional Indian medicine. Along with nutritive with anti-oxidant properties, it is, clinically, used in the treatment of various diseases with underlying anti-oxidant mechanism. This study is an effort to scrutinise the renal protective effect of P. amygdalus Batsch or green almond (GA) seed coat extract and its underlying mechanism in animal model of Ferric nitrilotriacetate (Fe-NTA) induced renal cell carcinoma (RCC). RCC was induced in Swiss Albino Wistar rats by intraperitoneal injection of Fe-NTA. The rats were then treated with ethanolic extract of GA (25, 50 and 100 mg/kg per oral) for 22 weeks. Efficacy of GA administration was evaluated by change in biochemical, renal, macroscopical and histopathological parameters and alterations. Additionally, interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inflammatory mediator including prostaglandin E2 (PGE 2 ), nuclear factor-kappa B (NF-κB) were also observed to explore the possible mechanisms. The oral administration of GA significantly (p Bowman capsules and inflammatory cells. Hence, it can be concluded that GA possesses observable chemo-protective action and effect on Fe-NTA induced RCC via dual inhibition mechanism one by inhibiting free radical generation and second by inhibiting inflammation.

  12. BlackBerry All-in-One for Dummies

    CERN Document Server

    Sarigumba, Dante; Petz, William

    2010-01-01

    Go beyond BlackBerry basics and get everything your BlackBerry can deliver. BlackBerry is the leading smartphone for business users, and its popularity continues to explode. When you discover the amazing array of BlackBerry possibilities in this fun and friendly guide, you'll be even happier with your choice of smartphones. BlackBerry All-in-One For Dummies explores every feature and application common to all BlackBerry devices. It explains the topics in depth, with tips, tricks, workarounds, and includes detailed information about cool new third-party applications, accessories, and downloads

  13. Instability of charged anti-de Sitter black holes

    International Nuclear Information System (INIS)

    Gwak, Bogeun; Lee, Bum-Hoon; Ro, Daeho

    2016-01-01

    We have studied the instability of charged anti-de Sitter black holes in four- or higher-dimensions under fragmentation. The unstable black holes under fragmentation can be broken into two black holes. Instability depends not only on the mass and charge of the black hole but also on the ratio between the fragmented black hole and its predecessor. We have found that the near extremal black holes are unstable, and Schwarzschild-AdS black holes are stable. These are qualitatively similar to black holes in four dimensions and higher. The detailed instabilities are numerically investigated.

  14. The Black Studies Boondoggle

    Science.gov (United States)

    Long, Richard A.

    1970-01-01

    Indicates tendencies dangerous to the basic purpose of Black Studies, and identifies four external challeges--imperialism, paternalism, nihilism, and materialism. An internal challenge is considered to be the use of European and Establishment constructs to analyze black reality. (DM)

  15. Black Films and Film-Makers.

    Science.gov (United States)

    Patterson, Lindsay, Ed.

    The development of black films and the attitudes of the film industry toward black films and black actors are some of the topics examined in this anthology of essays. Section 1, "Nigger to Supernigger," contains such articles as "The Death of Rastus: Negroes in American Films" by Thomas R. Cripps and "Folk Values in a New Medium" by Alain Locke…

  16. Factors affecting branch wound occlusion and associated decay following pruning – a case study with wild cherry (Prunus avium L.

    Directory of Open Access Journals (Sweden)

    Jonathan Sheppard

    2016-11-01

    Full Text Available Pruning wild cherry (Prunus avium L. is a common silvicultural practice carried out to produce valuable timber at a veneer wood quality. Sub-optimal pruning treatments can permit un-occluded pruning wounds to develop devaluing decay. The aim of this study is to determine relevant branch, tree and pruning characteristics affecting the occlusion process of pruning wounds. Important factors influencing occlusion time for an optimised pruning treatment for valuable timber production utilising wild cherry are derived. 85 artificially pruned branches originating from ten wild cherry trees were retrospectively analysed. Branch stub length, branch diameter and radial stem increment during occlusion were found to be significant predictors for occlusion time. From the results it could be concluded that for the long term success of artificial pruning of wild cherry it is crucial to (i keep branch stubs short (while avoiding damage to the branch collar, (ii to enable the tree to maintain significant radial growth after pruning, (iii to avoid large pruning wounds (>2.5 cm by removing steeply angled and fast growing branches at an early stage.

  17. Black holes and cosmic censorship

    International Nuclear Information System (INIS)

    Hiscock, W.A.

    1979-01-01

    It is widely accepted that the complete gravitational collapse of a body always yields a black hole, and that naked singularities are never produced (the cosmic censorship hypothesis). The local (or strong) cosmic censorship hypothesis states that singularities which are even locally naked (e.g., to an observer inside a black hole) are never produced. This dissertation studies the validity of these two conjectures. The Kerr-Newman metrics describes the black holes only when M 2 greater than or equal to Q 2 + P 2 , where M is the mass of the black hole, a = J/M its specific angular momentum, Q its electric charge, and P its magnetic charge. In the first part of this dissertation, the possibility of converting an extreme Kerr-Newman black hole (M 2 = a 2 + Q 2 + P 2 ) into a naked singularity by the accretion of test particles is considered. The motion of test particles is studied with a large angular momentum to energy ratio, and also test particles with a large charge to energy ratio. The final state is always found to be a black hole if the angular momentum, electric charge, and magnetic charge of the black hole are all much greater than the corresponding angular momentum, electric charge, and magnetic charge of the test particle. In Part II of this dissertation possible black hole interior solutions are studied. The Cauchy horizons and locally naked timelike singularities of the charged (and/or rotating) solutions are contrasted with the spacelike all-encompassing singularity of the Schwarzschild solution. It is determined which portions of the analytic extension of the Reissner-Nordstroem solution are relevant to realistic gravitational collapse

  18. Selective coating for solar panels. [using black chrome and black nickel

    Science.gov (United States)

    Mcdonald, G. E. (Inventor)

    1977-01-01

    The energy absorbing properties of solar heating panels are improved by depositing a black chrome coating of controlled thickness on a specially prepared surface of a metal substrate. The surface is prepared by depositing a dull nickel on the substrate, and the black chrome is plated on this low emittance surface to a thickness between 0.5 micron and 2.5 microns.

  19. Quantum capacity of quantum black holes

    Science.gov (United States)

    Adami, Chris; Bradler, Kamil

    2014-03-01

    The fate of quantum entanglement interacting with a black hole has been an enduring mystery, not the least because standard curved space field theory does not address the interaction of black holes with matter. We discuss an effective Hamiltonian of matter interacting with a black hole that has a precise analogue in quantum optics and correctly reproduces both spontaneous and stimulated Hawking radiation with grey-body factors. We calculate the quantum capacity of this channel in the limit of perfect absorption, as well as in the limit of a perfectly reflecting black hole (a white hole). We find that the white hole is an optimal quantum cloner, and is isomorphic to the Unruh channel with positive quantum capacity. The complementary channel (across the horizon) is entanglement-breaking with zero capacity, avoiding a violation of the quantum no-cloning theorem. The black hole channel on the contrary has vanishing capacity, while its complement has positive capacity instead. Thus, quantum states can be reconstructed faithfully behind the black hole horizon, but not outside. This work sheds new light on black hole complementarity because it shows that black holes can both reflect and absorb quantum states without violating the no-cloning theorem, and makes quantum firewalls obsolete.

  20. Black Edens, country Eves: Listening, performance, and black queer longing in country music.

    Science.gov (United States)

    Royster, Francesca T

    2017-07-03

    This article explores Black queer country music listening, performance, and fandom as a source of pleasure, nostalgia, and longing for Black listeners. Country music can be a space for alliance and community, as well as a way of accessing sometimes repressed cultural and personal histories of violence: lynching and other forms of racial terror, gender surveillance and disciplining, and continued racial and economic segregation. For many Black country music listeners and performers, the experience of being a closeted fan also fosters an experience of ideological hailing, as well as queer world-making. Royster suggests that through Black queer country music fandom and performance, fans construct risky and soulful identities. The article uses Tina Turner's solo album, Tina Turns the Country On! (1974) as an example of country music's power as a tool for resistance to racial, sexual, and class disciplining.