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Sample records for proton-driven flagellar motor

  1. Exchange of rotor components in functioning bacterial flagellar motor

    International Nuclear Information System (INIS)

    Fukuoka, Hajime; Inoue, Yuichi; Terasawa, Shun; Takahashi, Hiroto; Ishijima, Akihiko

    2010-01-01

    The bacterial flagellar motor is a rotary motor driven by the electrochemical potential of a coupling ion. The interaction between a rotor and stator units is thought to generate torque. The overall structure of flagellar motor has been thought to be static, however, it was recently proved that stators are exchanged in a rotating motor. Understanding the dynamics of rotor components in functioning motor is important for the clarifying of working mechanism of bacterial flagellar motor. In this study, we focused on the dynamics and the turnover of rotor components in a functioning flagellar motor. Expression systems for GFP-FliN, FliM-GFP, and GFP-FliG were constructed, and each GFP-fusion was functionally incorporated into the flagellar motor. To investigate whether the rotor components are exchanged in a rotating motor, we performed fluorescence recovery after photobleaching experiments using total internal reflection fluorescence microscopy. After photobleaching, in a tethered cell producing GFP-FliN or FliM-GFP, the recovery of fluorescence at the rotational center was observed. However, in a cell producing GFP-FliG, no recovery of fluorescence was observed. The transition phase of fluorescence intensity after full or partially photobleaching allowed the turnover of FliN subunits to be calculated as 0.0007 s -1 , meaning that FliN would be exchanged in tens of minutes. These novel findings indicate that a bacterial flagellar motor is not a static structure even in functioning state. This is the first report for the exchange of rotor components in a functioning bacterial flagellar motor.

  2. An Element of Determinism in a Stochastic Flagellar Motor Switch.

    Science.gov (United States)

    Xie, Li; Altindal, Tuba; Wu, Xiao-Lun

    2015-01-01

    Marine bacterium Vibrio alginolyticus uses a single polar flagellum to navigate in an aqueous environment. Similar to Escherichia coli cells, the polar flagellar motor has two states; when the motor is counter-clockwise, the cell swims forward and when the motor is clockwise, the cell swims backward. V. alginolyticus also incorporates a direction randomization step at the start of the forward swimming interval by flicking its flagellum. To gain an understanding on how the polar flagellar motor switch is regulated, distributions of the forward Δf and backward Δb intervals are investigated herein. We found that the steady-state probability density functions, P(Δf) and P(Δb), of freely swimming bacteria are strongly peaked at a finite time, suggesting that the motor switch is not Poissonian. The short-time inhibition is sufficiently strong and long lasting, i.e., several hundred milliseconds for both intervals, which is readily observed and characterized. Treating motor reversal dynamics as a first-passage problem, which results from conformation fluctuations of the motor switch, we calculated P(Δf) and P(Δb) and found good agreement with the measurements.

  3. Mechanics of torque generation in the bacterial flagellar motor.

    Science.gov (United States)

    Mandadapu, Kranthi K; Nirody, Jasmine A; Berry, Richard M; Oster, George

    2015-08-11

    The bacterial flagellar motor (BFM) is responsible for driving bacterial locomotion and chemotaxis, fundamental processes in pathogenesis and biofilm formation. In the BFM, torque is generated at the interface between transmembrane proteins (stators) and a rotor. It is well established that the passage of ions down a transmembrane gradient through the stator complex provides the energy for torque generation. However, the physics involved in this energy conversion remain poorly understood. Here we propose a mechanically specific model for torque generation in the BFM. In particular, we identify roles for two fundamental forces involved in torque generation: electrostatic and steric. We propose that electrostatic forces serve to position the stator, whereas steric forces comprise the actual "power stroke." Specifically, we propose that ion-induced conformational changes about a proline "hinge" residue in a stator α-helix are directly responsible for generating the power stroke. Our model predictions fit well with recent experiments on a single-stator motor. The proposed model provides a mechanical explanation for several fundamental properties of the flagellar motor, including torque-speed and speed-ion motive force relationships, backstepping, variation in step sizes, and the effects of key mutations in the stator.

  4. Model Studies of the Dynamics of Bacterial Flagellar Motors

    Energy Technology Data Exchange (ETDEWEB)

    Bai, F; Lo, C; Berry, R; Xing, J

    2009-03-19

    The Bacterial Flagellar Motor is a rotary molecular machine that rotates the helical filaments which propel swimming bacteria. Extensive experimental and theoretical studies exist on the structure, assembly, energy input, power generation and switching mechanism of the motor. In our previous paper, we explained the general physics underneath the observed torque-speed curves with a simple two-state Fokker-Planck model. Here we further analyze this model. In this paper we show (1) the model predicts that the two components of the ion motive force can affect the motor dynamics differently, in agreement with the latest experiment by Lo et al.; (2) with explicit consideration of the stator spring, the model also explains the lack of dependence of the zero-load speed on stator number in the proton motor, recently observed by Yuan and Berg; (3) the model reproduces the stepping behavior of the motor even with the existence of the stator springs and predicts the dwelling time distribution. Predicted stepping behavior of motors with two stators is discussed, and we suggest future experimental verification.

  5. Model Studies of the Dynamics of Bacterial Flagellar Motors

    Science.gov (United States)

    Bai, Fan; Lo, Chien-Jung; Berry, Richard M.; Xing, Jianhua

    2009-01-01

    Abstract The bacterial flagellar motor is a rotary molecular machine that rotates the helical filaments that propel swimming bacteria. Extensive experimental and theoretical studies exist on the structure, assembly, energy input, power generation, and switching mechanism of the motor. In a previous article, we explained the general physics underneath the observed torque-speed curves with a simple two-state Fokker-Planck model. Here, we further analyze that model, showing that 1), the model predicts that the two components of the ion motive force can affect the motor dynamics differently, in agreement with latest experiments; 2), with explicit consideration of the stator spring, the model also explains the lack of dependence of the zero-load speed on stator number in the proton motor, as recently observed; and 3), the model reproduces the stepping behavior of the motor even with the existence of the stator springs and predicts the dwell-time distribution. The predicted stepping behavior of motors with two stators is discussed, and we suggest future experimental procedures for verification. PMID:19383460

  6. Impact of fluorescent protein fusions on the bacterial flagellar motor.

    Science.gov (United States)

    Heo, M; Nord, A L; Chamousset, D; van Rijn, E; Beaumont, H J E; Pedaci, F

    2017-10-03

    Fluorescent fusion proteins open a direct and unique window onto protein function. However, they also introduce the risk of perturbation of the function of the native protein. Successful applications of fluorescent fusions therefore rely on a careful assessment and minimization of the side effects, but such insight is still lacking for many applications. This is particularly relevant in the study of the internal dynamics of motor proteins, where both the chemical and mechanical reaction coordinates can be affected. Fluorescent proteins fused to the stator of the Bacterial Flagellar Motor (BFM) have previously been used to unveil the motor subunit dynamics. Here we report the effects on single motors of three fluorescent proteins fused to the stators, all of which altered BFM behavior. The torque generated by individual stators was reduced while their stoichiometry remained unaffected. MotB fusions decreased the switching frequency and induced a novel bias-dependent asymmetry in the speed in the two directions. These effects could be mitigated by inserting a linker at the fusion point. These findings provide a quantitative account of the effects of fluorescent fusions to the stator on BFM dynamics and their alleviation- new insights that advance the use of fluorescent fusions to probe the dynamics of protein complexes.

  7. Applying torque to the Escherichia coli flagellar motor using magnetic tweezers

    NARCIS (Netherlands)

    van Oene, M.M.; Dickinson, L.E.; Cross, B.; Pedaci, F.; Lipfert, J.; Dekker, N.H.

    2017-01-01

    The bacterial flagellar motor of Escherichia coli is a nanoscale rotary engine essential for bacterial propulsion. Studies on the power output of single motors rely on the measurement of motor torque and rotation under external load. Here, we investigate the use of magnetic tweezers, which in

  8. Applying torque to the Escherichia coli flagellar motor using magnetic tweezers

    Science.gov (United States)

    van Oene, Maarten M.; Dickinson, Laura E.; Cross, Bronwen; Pedaci, Francesco; Lipfert, Jan; Dekker, Nynke H.

    2017-01-01

    The bacterial flagellar motor of Escherichia coli is a nanoscale rotary engine essential for bacterial propulsion. Studies on the power output of single motors rely on the measurement of motor torque and rotation under external load. Here, we investigate the use of magnetic tweezers, which in principle allow the application and active control of a calibrated load torque, to study single flagellar motors in Escherichia coli. We manipulate the external load on the motor by adjusting the magnetic field experienced by a magnetic bead linked to the motor, and we probe the motor’s response. A simple model describes the average motor speed over the entire range of applied fields. We extract the motor torque at stall and find it to be similar to the motor torque at drag-limited speed. In addition, use of the magnetic tweezers allows us to force motor rotation in both forward and backward directions. We monitor the motor’s performance before and after periods of forced rotation and observe no destructive effects on the motor. Our experiments show how magnetic tweezers can provide active and fast control of the external load while also exposing remaining challenges in calibration. Through their non-invasive character and straightforward parallelization, magnetic tweezers provide an attractive platform to study nanoscale rotary motors at the single-motor level. PMID:28266562

  9. Bio-hybrid micro/nanodevices powered by flagellar motor: challenges and strategies

    Directory of Open Access Journals (Sweden)

    Jin-Woo eKim

    2015-07-01

    Full Text Available Molecular motors, which are precision-engineered by nature, offer exciting possibilities for bio-hybrid engineered systems. They could enable real applications ranging from micro/nano fluidics, to biosensing, to medical diagnoses. This review describes the fundamental biological insights and fascinating potentials of these remarkable sensing and actuation machines, in particular bacterial flagellar motors, as well as their engineering perspectives with regard to applications in bio-engineered hybrid systems and nanobiotechnology.

  10. Speed of the bacterial flagellar motor near zero load depends on the number of stator units.

    Science.gov (United States)

    Nord, Ashley L; Sowa, Yoshiyuki; Steel, Bradley C; Lo, Chien-Jung; Berry, Richard M

    2017-10-31

    The bacterial flagellar motor (BFM) rotates hundreds of times per second to propel bacteria driven by an electrochemical ion gradient. The motor consists of a rotor 50 nm in diameter surrounded by up to 11 ion-conducting stator units, which exchange between motors and a membrane-bound pool. Measurements of the torque-speed relationship guide the development of models of the motor mechanism. In contrast to previous reports that speed near zero torque is independent of the number of stator units, we observe multiple speeds that we attribute to different numbers of units near zero torque in both Na + - and H + -driven motors. We measure the full torque-speed relationship of one and two H + units in Escherichia coli by selecting the number of H + units and controlling the number of Na + units in hybrid motors. These experiments confirm that speed near zero torque in H + -driven motors increases with the stator number. We also measured 75 torque-speed curves for Na + -driven chimeric motors at different ion-motive force and stator number. Torque and speed were proportional to ion-motive force and number of stator units at all loads, allowing all 77 measured torque-speed curves to be collapsed onto a single curve by simple rescaling. Published under the PNAS license.

  11. Temperature Dependences of Torque Generation and Membrane Voltage in the Bacterial Flagellar Motor

    Science.gov (United States)

    Inoue, Yuichi; Baker, Matthew A.B.; Fukuoka, Hajime; Takahashi, Hiroto; Berry, Richard M.; Ishijima, Akihiko

    2013-01-01

    In their natural habitats bacteria are frequently exposed to sudden changes in temperature that have been shown to affect their swimming. With our believed to be new methods of rapid temperature control for single-molecule microscopy, we measured here the thermal response of the Na+-driven chimeric motor expressed in Escherichia coli cells. Motor torque at low load (0.35 μm bead) increased linearly with temperature, twofold between 15°C and 40°C, and torque at high load (1.0 μm bead) was independent of temperature, as reported for the H+-driven motor. Single cell membrane voltages were measured by fluorescence imaging and these were almost constant (∼120 mV) over the same temperature range. When the motor was heated above 40°C for 1–2 min the torque at high load dropped reversibly, recovering upon cooling below 40°C. This response was repeatable over as many as 10 heating cycles. Both increases and decreases in torque showed stepwise torque changes with unitary size ∼150 pN nm, close to the torque of a single stator at room temperature (∼180 pN nm), indicating that dynamic stator dissociation occurs at high temperature, with rebinding upon cooling. Our results suggest that the temperature-dependent assembly of stators is a general feature of flagellar motors. PMID:24359752

  12. Coevolved Mutations Reveal Distinct Architectures for Two Core Proteins in the Bacterial Flagellar Motor.

    Directory of Open Access Journals (Sweden)

    Alessandro Pandini

    Full Text Available Switching of bacterial flagellar rotation is caused by large domain movements of the FliG protein triggered by binding of the signal protein CheY to FliM. FliG and FliM form adjacent multi-subunit arrays within the basal body C-ring. The movements alter the interaction of the FliG C-terminal (FliGC "torque" helix with the stator complexes. Atomic models based on the Salmonella entrovar C-ring electron microscopy reconstruction have implications for switching, but lack consensus on the relative locations of the FliG armadillo (ARM domains (amino-terminal (FliGN, middle (FliGM and FliGC as well as changes during chemotaxis. The generality of the Salmonella model is challenged by the variation in motor morphology and response between species. We studied coevolved residue mutations to determine the unifying elements of switch architecture. Residue interactions, measured by their coevolution, were formalized as a network, guided by structural data. Our measurements reveal a common design with dedicated switch and motor modules. The FliM middle domain (FliMM has extensive connectivity most simply explained by conserved intra and inter-subunit contacts. In contrast, FliG has patchy, complex architecture. Conserved structural motifs form interacting nodes in the coevolution network that wire FliMM to the FliGC C-terminal, four-helix motor module (C3-6. FliG C3-6 coevolution is organized around the torque helix, differently from other ARM domains. The nodes form separated, surface-proximal patches that are targeted by deleterious mutations as in other allosteric systems. The dominant node is formed by the EHPQ motif at the FliMMFliGM contact interface and adjacent helix residues at a central location within FliGM. The node interacts with nodes in the N-terminal FliGc α-helix triad (ARM-C and FliGN. ARM-C, separated from C3-6 by the MFVF motif, has poor intra-network connectivity consistent with its variable orientation revealed by structural data. ARM

  13. Proton-driven Plasma Wakefield Acceleration

    CERN Multimedia

    CERN. Geneva

    2012-01-01

    The construction of ever larger and costlier accelerator facilities has a limited future, and new technologies will be needed to push the energy frontier. Plasma wakefield acceleration is a rapidly developing field and is a promising candidate technology for future high energy colliders. We focus on the recently proposed idea of proton-driven plasma wakefield acceleration and describe the current status and plans for this approach.

  14. Crystallization and preliminary X-ray analysis of the flagellar motor `brake' molecule YcgR with c-di-GMP from Escherichia coli.

    Science.gov (United States)

    Hou, Yanjie; Li, De Feng; Wang, Da Cheng

    2013-06-01

    In Escherichia coli and Salmonella enterica, bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP), a ubiquitous bacterial second-messenger molecule that participates in many cellular processes, can regulate flagellar motor speed and reduce cell swimming velocity by binding to the PilZ-containing protein YcgR. Here, the crystallization and preliminary X-ray crystallographic analysis of YcgR with c-di-GMP are reported. The crystals diffracted to 2.3 Å resolution and belonged to space group R3:H, with unit-cell parameters a = b = 93.96, c = 109.61 Å. The asymmetric unit appeared to contain one subunit with a Matthews coefficient of 3.21 Å(3) Da(-1). The results reported here provide a sound basis for solving the crystal structure of YcgR with c-di-GMP and revealing its structure-function relationship based on the three-dimensional structure.

  15. Crystallographic and molecular dynamics analysis of loop motions unmasking the peptidoglycan-binding site in stator protein MotB of flagellar motor.

    Directory of Open Access Journals (Sweden)

    Cyril F Reboul

    Full Text Available BACKGROUND: The C-terminal domain of MotB (MotB-C shows high sequence similarity to outer membrane protein A and related peptidoglycan (PG-binding proteins. It is believed to anchor the power-generating MotA/MotB stator unit of the bacterial flagellar motor to the peptidoglycan layer of the cell wall. We previously reported the first crystal structure of this domain and made a puzzling observation that all conserved residues that are thought to be essential for PG recognition are buried and inaccessible in the crystal structure. In this study, we tested a hypothesis that peptidoglycan binding is preceded by, or accompanied by, some structural reorganization that exposes the key conserved residues. METHODOLOGY/PRINCIPAL FINDINGS: We determined the structure of a new crystalline form (Form B of Helicobacter pylori MotB-C. Comparisons with the existing Form A revealed conformational variations in the petal-like loops around the carbohydrate binding site near one end of the β-sheet. These variations are thought to reflect natural flexibility at this site required for insertion into the peptidoglycan mesh. In order to understand the nature of this flexibility we have performed molecular dynamics simulations of the MotB-C dimer. The results are consistent with the crystallographic data and provide evidence that the three loops move in a concerted fashion, exposing conserved MotB residues that have previously been implicated in binding of the peptide moiety of peptidoglycan. CONCLUSION/SIGNIFICANCE: Our structural analysis provides a new insight into the mechanism by which MotB inserts into the peptidoglycan mesh, thus anchoring the power-generating complex to the cell wall.

  16. Inactivation of ferric uptake regulator (Fur) attenuates Helicobacter pylori J99 motility by disturbing the flagellar motor switch and autoinducer-2 production.

    Science.gov (United States)

    Lee, Ai-Yun; Kao, Cheng-Yen; Wang, Yao-Kuan; Lin, Ssu-Yuan; Lai, Tze-Ying; Sheu, Bor-Shyang; Lo, Chien-Jung; Wu, Jiunn-Jong

    2017-08-01

    Flagellar motility of Helicobacter pylori has been shown to be important for the bacteria to establish initial colonization. The ferric uptake regulator (Fur) is a global regulator that has been identified in H. pylori which is involved in the processes of iron uptake and establishing colonization. However, the role of Fur in H. pylori motility is still unclear. Motility of the wild-type, fur mutant, and fur revertant J99 were determined by a soft-agar motility assay and direct video observation. The bacterial shape and flagellar structure were evaluated by transmission electron microscopy. Single bacterial motility and flagellar switching were observed by phase-contrast microscopy. Autoinducer-2 (AI-2) production in bacterial culture supernatant was analyzed by a bioluminescence assay. The fur mutant showed impaired motility in the soft-agar assay compared with the wild-type J99 and fur revertant. The numbers and lengths of flagellar filaments on the fur mutant cells were similar to those of the wild-type and revertant cells. Phenotypic characterization showed similar swimming speed but reduction in switching rate in the fur mutant. The AI-2 production of the fur mutant was dramatically reduced compared with wild-type J99 in log-phase culture medium. These results indicate that Fur positively modulates H. pylori J99 motility through interfering with bacterial flagellar switching. © 2017 John Wiley & Sons Ltd.

  17. H(+) and Na(+) are involved in flagellar rotation of the spirochete Leptospira.

    Science.gov (United States)

    Islam, Md Shafiqul; Morimoto, Yusuke V; Kudo, Seishi; Nakamura, Shuichi

    2015-10-16

    Leptospira is a spirochete possessing intracellular flagella. Each Leptospira flagellar filament is linked with a flagellar motor composed of a rotor and a dozen stators. For many bacterial species, it is known that the stator functions as an ion channel and that the ion flux through the stator is coupled with flagellar rotation. The coupling ion varies depending on the species; for example, H(+) is used in Escherichia coli, and Na(+) is used in Vibrio spp. to drive a polar flagellum. Although genetic and structural studies illustrated that the Leptospira flagellar motor also contains a stator, the coupling ion for flagellar rotation remains unknown. In the present study, we analyzed the motility of Leptospira under various pH values and salt concentrations. Leptospira cells displayed motility in acidic to alkaline pH. In the presence of a protonophore, the cells completely lost motility in acidic to neutral pH but displayed extremely slow movement under alkaline conditions. This result suggests that H(+) is a major coupling ion for flagellar rotation over a wide pH range; however, we also observed that the motility of Leptospira was significantly enhanced by the addition of Na(+), though it vigorously moved even under Na(+)-free conditions. These results suggest that H(+) is preferentially used and that Na(+) is secondarily involved in flagellar rotation in Leptospira. The flexible ion selectivity in the flagellar system could be advantageous for Leptospira to survive in a wide range of environment. Copyright © 2015 Elsevier Inc. All rights reserved.

  18. Second-chance signal transduction explains cooperative flagellar switching.

    Science.gov (United States)

    Zot, Henry G; Hasbun, Javier E; Minh, Nguyen Van

    2012-01-01

    The reversal of flagellar motion (switching) results from the interaction between a switch complex of the flagellar rotor and a torque-generating stationary unit, or stator (motor unit). To explain the steeply cooperative ligand-induced switching, present models propose allosteric interactions between subunits of the rotor, but do not address the possibility of a reaction that stimulates a bidirectional motor unit to reverse direction of torque. During flagellar motion, the binding of a ligand-bound switch complex at the dwell site could excite a motor unit. The probability that another switch complex of the rotor, moving according to steady-state rotation, will reach the same dwell site before that motor unit returns to ground state will be determined by the independent decay rate of the excited-state motor unit. Here, we derive an analytical expression for the energy coupling between a switch complex and a motor unit of the stator complex of a flagellum, and demonstrate that this model accounts for the cooperative switching response without the need for allosteric interactions. The analytical result can be reproduced by simulation when (1) the motion of the rotor delivers a subsequent ligand-bound switch to the excited motor unit, thereby providing the excited motor unit with a second chance to remain excited, and (2) the outputs from multiple independent motor units are constrained to a single all-or-none event. In this proposed model, a motor unit and switch complex represent the components of a mathematically defined signal transduction mechanism in which energy coupling is driven by steady-state and is regulated by stochastic ligand binding. Mathematical derivation of the model shows the analytical function to be a general form of the Hill equation (Hill AV (1910) The possible effects of the aggregation of the molecules of haemoglobin on its dissociation curves. J Physiol 40: iv-vii).

  19. H+ and Na+ are involved in flagellar rotation of the spirochete Leptospira

    International Nuclear Information System (INIS)

    Islam, Md. Shafiqul; Morimoto, Yusuke V.; Kudo, Seishi; Nakamura, Shuichi

    2015-01-01

    Leptospira is a spirochete possessing intracellular flagella. Each Leptospira flagellar filament is linked with a flagellar motor composed of a rotor and a dozen stators. For many bacterial species, it is known that the stator functions as an ion channel and that the ion flux through the stator is coupled with flagellar rotation. The coupling ion varies depending on the species; for example, H + is used in Escherichia coli, and Na + is used in Vibrio spp. to drive a polar flagellum. Although genetic and structural studies illustrated that the Leptospira flagellar motor also contains a stator, the coupling ion for flagellar rotation remains unknown. In the present study, we analyzed the motility of Leptospira under various pH values and salt concentrations. Leptospira cells displayed motility in acidic to alkaline pH. In the presence of a protonophore, the cells completely lost motility in acidic to neutral pH but displayed extremely slow movement under alkaline conditions. This result suggests that H + is a major coupling ion for flagellar rotation over a wide pH range; however, we also observed that the motility of Leptospira was significantly enhanced by the addition of Na + , though it vigorously moved even under Na + -free conditions. These results suggest that H + is preferentially used and that Na + is secondarily involved in flagellar rotation in Leptospira. The flexible ion selectivity in the flagellar system could be advantageous for Leptospira to survive in a wide range of environment. - Highlights: • This is a study on input energy for motility in the spirochete Leptospira. • Leptospira biflexa exhibited active motility in acidic to alkaline pH. • Both H + and Na + are involved in flagellar rotation in Leptospira. • H + is a primary energy source, but Na + can secondarily enhance motility.

  20. H{sup +} and Na{sup +} are involved in flagellar rotation of the spirochete Leptospira

    Energy Technology Data Exchange (ETDEWEB)

    Islam, Md. Shafiqul [Department of Applied Physics, Graduate School of Engineering, Tohoku University, 6-6-05 Aoba, Aoba-ku, Sendai, Miyagi 980-8579 (Japan); Morimoto, Yusuke V. [Quantitative Biology Center, RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874 (Japan); Graduate School of Frontier BioSciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871 (Japan); Kudo, Seishi [Department of Applied Physics, Graduate School of Engineering, Tohoku University, 6-6-05 Aoba, Aoba-ku, Sendai, Miyagi 980-8579 (Japan); Nakamura, Shuichi, E-mail: naka@bp.apph.tohoku.ac.jp [Department of Applied Physics, Graduate School of Engineering, Tohoku University, 6-6-05 Aoba, Aoba-ku, Sendai, Miyagi 980-8579 (Japan); Graduate School of Frontier BioSciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871 (Japan)

    2015-10-16

    Leptospira is a spirochete possessing intracellular flagella. Each Leptospira flagellar filament is linked with a flagellar motor composed of a rotor and a dozen stators. For many bacterial species, it is known that the stator functions as an ion channel and that the ion flux through the stator is coupled with flagellar rotation. The coupling ion varies depending on the species; for example, H{sup +} is used in Escherichia coli, and Na{sup +} is used in Vibrio spp. to drive a polar flagellum. Although genetic and structural studies illustrated that the Leptospira flagellar motor also contains a stator, the coupling ion for flagellar rotation remains unknown. In the present study, we analyzed the motility of Leptospira under various pH values and salt concentrations. Leptospira cells displayed motility in acidic to alkaline pH. In the presence of a protonophore, the cells completely lost motility in acidic to neutral pH but displayed extremely slow movement under alkaline conditions. This result suggests that H{sup +} is a major coupling ion for flagellar rotation over a wide pH range; however, we also observed that the motility of Leptospira was significantly enhanced by the addition of Na{sup +}, though it vigorously moved even under Na{sup +}-free conditions. These results suggest that H{sup +} is preferentially used and that Na{sup +} is secondarily involved in flagellar rotation in Leptospira. The flexible ion selectivity in the flagellar system could be advantageous for Leptospira to survive in a wide range of environment. - Highlights: • This is a study on input energy for motility in the spirochete Leptospira. • Leptospira biflexa exhibited active motility in acidic to alkaline pH. • Both H{sup +} and Na{sup +} are involved in flagellar rotation in Leptospira. • H{sup +} is a primary energy source, but Na{sup +} can secondarily enhance motility.

  1. AWAKE, The Advanced Proton Driven Plasma Wakefield Acceleration Experiment at CERN

    CERN Document Server

    Gschwendtner, E.; Amorim, L.; Apsimon, R.; Assmann, R.; Bachmann, A.M.; Batsch, F.; Bauche, J.; Berglyd Olsen, V.K.; Bernardini, M.; Bingham, R.; Biskup, B.; Bohl, T.; Bracco, C.; Burrows, P.N.; Burt, G.; Buttenschon, B.; Butterworth, A.; Caldwell, A.; Cascella, M.; Chevallay, E.; Cipiccia, S.; Damerau, H.; Deacon, L.; Dirksen, P.; Doebert, S.; Dorda, U.; Farmer, J.; Fedosseev, V.; Feldbaumer, E.; Fiorito, R.; Fonseca, R.; Friebel, F.; Gorn, A.A.; Grulke, O.; Hansen, J.; Hessler, C.; Hofle, W.; Holloway, J.; Huther, M.; Jaroszynski, D.; Jensen, L.; Jolly, S.; Joulaei, A.; Kasim, M.; Keeble, F.; Li, Y.; Liu, S.; Lopes, N.; Lotov, K.V.; Mandry, S.; Martorelli, R.; Martyanov, M.; Mazzoni, S.; Mete, O.; Minakov, V.A.; Mitchell, J.; Moody, J.; Muggli, P.; Najmudin, Z.; Norreys, P.; Oz, E.; Pardons, A.; Pepitone, K.; Petrenko, A.; Plyushchev, G.; Pukhov, A.; Rieger, K.; Ruhl, H.; Salveter, F.; Savard, N.; Schmidt, J.; Seryi, A.; Shaposhnikova, E.; Sheng, Z.M.; Sherwood, P.; Silva, L.; Soby, L.; Sosedkin, A.P.; Spitsyn, R.I.; Trines, R.; Tuev, P.V.; Turner, M.; Verzilov, V.; Vieira, J.; Vincke, H.; Wei, Y.; Welsch, C.P.; Wing, M.; Xia, G.; Zhang, H.

    2016-01-01

    The Advanced Proton Driven Plasma Wakefield Acceleration Experiment (AWAKE) aims at studying plasma wakefield generation and electron acceleration driven by proton bunches. It is a proof-of-principle R&D experiment at CERN and the world's first proton driven plasma wakefield acceleration experiment. The AWAKE experiment will be installed in the former CNGS facility and uses the 400 GeV/c proton beam bunches from the SPS. The first experiments will focus on the self-modulation instability of the long (rms ~12 cm) proton bunch in the plasma. These experiments are planned for the end of 2016. Later, in 2017/2018, low energy (~15 MeV) electrons will be externally injected to sample the wakefields and be accelerated beyond 1 GeV. The main goals of the experiment will be summarized. A summary of the AWAKE design and construction status will be presented.

  2. Nonlinear amplitude dynamics in flagellar beating.

    Science.gov (United States)

    Oriola, David; Gadêlha, Hermes; Casademunt, Jaume

    2017-03-01

    The physical basis of flagellar and ciliary beating is a major problem in biology which is still far from completely understood. The fundamental cytoskeleton structure of cilia and flagella is the axoneme, a cylindrical array of microtubule doublets connected by passive cross-linkers and dynein motor proteins. The complex interplay of these elements leads to the generation of self-organized bending waves. Although many mathematical models have been proposed to understand this process, few attempts have been made to assess the role of dyneins on the nonlinear nature of the axoneme. Here, we investigate the nonlinear dynamics of flagella by considering an axonemal sliding control mechanism for dynein activity. This approach unveils the nonlinear selection of the oscillation amplitudes, which are typically either missed or prescribed in mathematical models. The explicit set of nonlinear equations are derived and solved numerically. Our analysis reveals the spatio-temporal dynamics of dynein populations and flagellum shape for different regimes of motor activity, medium viscosity and flagellum elasticity. Unstable modes saturate via the coupling of dynein kinetics and flagellum shape without the need of invoking a nonlinear axonemal response. Hence, our work reveals a novel mechanism for the saturation of unstable modes in axonemal beating.

  3. Nanobody-Displaying Flagellar Nanotubes.

    Science.gov (United States)

    Klein, Ágnes; Kovács, Mátyás; Muskotál, Adél; Jankovics, Hajnalka; Tóth, Balázs; Pósfai, Mihály; Vonderviszt, Ferenc

    2018-02-26

    In this work we addressed the problem how to fabricate self-assembling tubular nanostructures displaying target recognition functionalities. Bacterial flagellar filaments, composed of thousands of flagellin subunits, were used as scaffolds to display single-domain antibodies (nanobodies) on their surface. As a representative example, an anti-GFP nanobody was successfully inserted into the middle part of flagellin replacing the hypervariable surface-exposed D3 domain. A novel procedure was developed to select appropriate linkers required for functional internal insertion. Linkers of various lengths and conformational properties were chosen from a linker database and they were randomly attached to both ends of an anti-GFP nanobody to facilitate insertion. Functional fusion constructs capable of forming filaments on the surface of flagellin-deficient host cells were selected by magnetic microparticles covered by target GFP molecules and appropriate linkers were identified. TEM studies revealed that short filaments of 2-900 nm were formed on the cell surface. ITC and fluorescent measurements demonstrated that the fusion protein exhibited high binding affinity towards GFP. Our approach allows the development of functionalized flagellar nanotubes against a variety of important target molecules offering potential applications in biosensorics and bio-nanotechnology.

  4. High-quality electron beam generation in a proton-driven hollow plasma wakefield accelerator

    Science.gov (United States)

    Li, Y.; Xia, G.; Lotov, K. V.; Sosedkin, A. P.; Hanahoe, K.; Mete-Apsimon, O.

    2017-10-01

    Simulations of proton-driven plasma wakefield accelerators have demonstrated substantially higher accelerating gradients compared to conventional accelerators and the viability of accelerating electrons to the energy frontier in a single plasma stage. However, due to the strong intrinsic transverse fields varying both radially and in time, the witness beam quality is still far from suitable for practical application in future colliders. Here we demonstrate the efficient acceleration of electrons in proton-driven wakefields in a hollow plasma channel. In this regime, the witness bunch is positioned in the region with a strong accelerating field, free from plasma electrons and ions. We show that the electron beam carrying the charge of about 10% of 1 TeV proton driver charge can be accelerated to 0.6 TeV with a preserved normalized emittance in a single channel of 700 m. This high-quality and high-charge beam may pave the way for the development of future plasma-based energy frontier colliders.

  5. Flagellar glycosylation in Clostridium botulinum.

    Science.gov (United States)

    Twine, Susan M; Paul, Catherine J; Vinogradov, Evgeny; McNally, David J; Brisson, Jean-Robert; Mullen, James A; McMullin, David R; Jarrell, Harold C; Austin, John W; Kelly, John F; Logan, Susan M

    2008-09-01

    Flagellins from Clostridium botulinum were shown to be post-translationally modified with novel glycan moieties by top-down MS analysis of purified flagellin protein from strains of various toxin serotypes. Detailed analyses of flagellin from two strains of C. botulinum demonstrated that the protein is modified by a novel glycan moiety of mass 417 Da in O-linkage. Bioinformatic analysis of available C. botulinum genomes identified a flagellar glycosylation island containing homologs of genes recently identified in Campylobacter coli that have been shown to be responsible for the biosynthesis of legionaminic acid derivatives. Structural characterization of the carbohydrate moiety was completed utilizing both MS and NMR spectroscopy, and it was shown to be a novel legionaminic acid derivative, 7-acetamido-5-(N-methyl-glutam-4-yl)-amino-3,5,7,9-tetradeoxy-D-glycero-alpha-D-galacto-nonulosonic acid, (alphaLeg5GluNMe7Ac). Electron transfer dissociation MS with and without collision-activated dissociation was utilized to map seven sites of O-linked glycosylation, eliminating the need for chemical derivatization of tryptic peptides prior to analysis. Marker ions for novel glycans, as well as a unique C-terminal flagellin peptide marker ion, were identified in a top-down analysis of the intact protein. These ions have the potential for use in for rapid detection and discrimination of C. botulinum cells, indicating botulinum neurotoxin contamination. This is the first report of glycosylation of Gram-positive flagellar proteins by the 'sialic acid-like' nonulosonate sugar, legionaminic acid.

  6. Flagellar Synchronization Is a Simple Alternative to Cell Cycle Synchronization for Ciliary and Flagellar Studies.

    Science.gov (United States)

    Dutta, Soumita; Avasthi, Prachee

    2017-01-01

    The unicellular green alga Chlamydomonas reinhardtii is an ideal model organism for studies of ciliary function and assembly. In assays for biological and biochemical effects of various factors on flagellar structure and function, synchronous culture is advantageous for minimizing variability. Here, we have characterized a method in which 100% synchronization is achieved with respect to flagellar length but not with respect to the cell cycle. The method requires inducing flagellar regeneration by amputation of the entire cell population and limiting regeneration time. This results in a maximally homogeneous distribution of flagellar lengths at 3 h postamputation. We found that time-limiting new protein synthesis during flagellar synchronization limits variability in the unassembled pool of limiting flagellar protein and variability in flagellar length without affecting the range of cell volumes. We also found that long- and short-flagella mutants that regenerate normally require longer and shorter synchronization times, respectively. By minimizing flagellar length variability using a simple method requiring only hours and no changes in media, flagellar synchronization facilitates the detection of small changes in flagellar length resulting from both chemical and genetic perturbations in Chlamydomonas . This method increases our ability to probe the basic biology of ciliary size regulation and related disease etiologies. IMPORTANCE Cilia and flagella are highly conserved antenna-like organelles that found in nearly all mammalian cell types. They perform sensory and motile functions contributing to numerous physiological and developmental processes. Defects in their assembly and function are implicated in a wide range of human diseases ranging from retinal degeneration to cancer. Chlamydomonas reinhardtii is an algal model system for studying mammalian cilium formation and function. Here, we report a simple synchronization method that allows detection of small

  7. An $ep$ collider based on proton-driven plasma wakefield acceleration

    CERN Document Server

    Wing, M.; Mete, O.; Aimidula, A.; Welsch, C.; Chattopadhyay, S.; Mandry, S.

    2014-01-01

    Recent simulations have shown that a high-energy proton bunch can excite strong plasma wakefields and accelerate a bunch of electrons to the energy frontier in a single stage of acceleration. This scheme could lead to a future $ep$ collider using the LHC for the proton beam and a compact electron accelerator of length 170 m, producing electrons of energy up to 100 GeV. The parameters of such a collider are discussed as well as conceptual layouts within the CERN accelerator complex. The physics of plasma wakefield acceleration will also be introduced, with the AWAKE experiment, a proof of principle demonstration of proton-driven plasma wakefield acceleration, briefly reviewed, as well as the physics possibilities of such an $ep$ collider.

  8. Bacteria exploit a polymorphic instability of the flagellar filament to escape from traps.

    Science.gov (United States)

    Kühn, Marco J; Schmidt, Felix K; Eckhardt, Bruno; Thormann, Kai M

    2017-06-13

    Many bacterial species swim by rotating single polar helical flagella. Depending on the direction of rotation, they can swim forward or backward and change directions to move along chemical gradients but also to navigate their obstructed natural environment in soils, sediments, or mucus. When they get stuck, they naturally try to back out, but they can also resort to a radically different flagellar mode, which we discovered here. Using high-speed microscopy, we monitored the swimming behavior of the monopolarly flagellated species Shewanella putrefaciens with fluorescently labeled flagellar filaments at an agarose-glass interface. We show that, when a cell gets stuck, the polar flagellar filament executes a polymorphic change into a spiral-like form that wraps around the cell body in a spiral-like fashion and enables the cell to escape by a screw-like backward motion. Microscopy and modeling suggest that this propagation mode is triggered by an instability of the flagellum under reversal of the rotation and the applied torque. The switch is reversible and bacteria that have escaped the trap can return to their normal swimming mode by another reversal of motor direction. The screw-type flagellar arrangement enables a unique mode of propagation and, given the large number of polarly flagellated bacteria, we expect it to be a common and widespread escape or motility mode in complex and structured environments.

  9. Kinematics of flagellar swimming in Euglena gracilis: Helical trajectories and flagellar shapes.

    Science.gov (United States)

    Rossi, Massimiliano; Cicconofri, Giancarlo; Beran, Alfred; Noselli, Giovanni; DeSimone, Antonio

    2017-12-12

    The flagellar swimming of euglenids, which are propelled by a single anterior flagellum, is characterized by a generalized helical motion. The 3D nature of this swimming motion, which lacks some of the symmetries enjoyed by more common model systems, and the complex flagellar beating shapes that power it make its quantitative description challenging. In this work, we provide a quantitative, 3D, highly resolved reconstruction of the swimming trajectories and flagellar shapes of specimens of Euglena gracilis We achieved this task by using high-speed 2D image recordings taken with a conventional inverted microscope combined with a precise characterization of the helical motion of the cell body to lift the 2D data to 3D trajectories. The propulsion mechanism is discussed. Our results constitute a basis for future biophysical research on a relatively unexplored type of eukaryotic flagellar movement. Copyright © 2017 the Author(s). Published by PNAS.

  10. Emergence of flagellar beating from the collective behavior of individual ATP-powered dyneins

    OpenAIRE

    Namdeo, S.; Onck, P. R.

    2016-01-01

    Flagella are hair-like projections from the surface of eukaryotic cells, and they play an important role in many cellular functions, such as cell-motility. The beating of flagella is enabled by their internal architecture, the axoneme, and is powered by a dense distribution of motor proteins, dyneins. The dyneins deliver the required mechanical work through the hydrolysis of ATP. Although the dynein-ATP cycle, the axoneme microstructure, and the flagellar-beating kinematics are well studied, ...

  11. An intrinsically disordered linker controlling the formation and the stability of the bacterial flagellar hook

    OpenAIRE

    Barker, Clive S.; Meshcheryakova, Irina V.; Kostyukova, Alla S.; Freddolino, Peter L.; Samatey, Fadel A.

    2017-01-01

    Background In a macro-molecular complex, any minor change may prove detrimental. For a supra-molecular nano-machine like the bacterial flagellum, which consists of several distinct parts with specific characteristics, stability is important. During the rotation of the bacterial flagellar motor, which is located in the membrane, the flagella rotate at speeds between 200 and 2000 rpm, depending on the bacterial species. The hook substructure of the bacterial flagellum acts as a universal joint ...

  12. AWAKE Design Report: A Proton-Driven Plasma Wakefield Acceleration Experiment at CERN

    CERN Document Server

    Caldwell, A; Lotov, K; Muggli, P; Wing, M

    2013-01-01

    The AWAKE Collaboration has been formed in order to demonstrate proton driven plasma wakefield acceleration for the first time. This technology could lead to future colliders of high energy but of a much reduced length compared to proposed linear accelerators. The SPS proton beam in the CNGS facility will be injected into a 10m plasma cell where the long proton bunches will be modulated into significantly shorter micro-bunches. These micro-bunches will then initiate a strong wakefield in the plasma with peak fields above 1 GV/m that will be harnessed to accelerate a bunch of electrons from about 20MeV to the GeV scale within a few meters. The experimental program is based on detailed numerical simulations of beam and plasma interactions. The main accelerator components, the experimental area and infrastructure required as well as the plasma cell and the diagnostic equipment are discussed in detail. First protons to the experiment are expected at the end of 2015 and this will be followed by an initial 3–4 ye...

  13. Proton-driven plasma wakefield acceleration: a path to the future of high-energy particle physics

    CERN Document Server

    Assmann, R.; Bohl, T.; Bracco, C.; Buttenschon, B.; Butterworth, A.; Caldwell, A.; Chattopadhyay, S.; Cipiccia, S.; Feldbaumer, E.; Fonseca, R.A.; Goddard, B.; Gross, M.; Grulke, O.; Gschwendtner, E.; Holloway, J.; Huang, C.; Jaroszynski, D.; Jolly, S.; Kempkes, P.; Lopes, N.; Lotov, K.; Machacek, J.; Mandry, S.R.; McKenzie, J.W.; Meddahi, M.; Militsyn, B.L.; Moschuering, N.; Muggli, P.; Najmudin, Z.; Noakes, T.C.Q.; Norreys, P.A.; Oz, E.; Pardons, A.; Petrenko, A.; Pukhov, A.; Rieger, K.; Reimann, O.; Ruhl, H.; Shaposhnikova, E.; Silva, L.O.; Sosedkin, A.; Tarkeshian, R.; Trines, R.M.G.N.; Tuckmantel, T.; Vieira, J.; Vincke, H.; Wing, M.; Xia, G.

    2014-01-01

    New acceleration technology is mandatory for the future elucidation of fundamental particles and their interactions. A promising approach is to exploit the properties of plasmas. Past research has focused on creating large-amplitude plasma waves by injecting an intense laser pulse or an electron bunch into the plasma. However, the maximum energy gain of electrons accelerated in a single plasma stage is limited by the energy of the driver. Proton bunches are the most promising drivers of wakefields to accelerate electrons to the TeV energy scale in a single stage. An experimental program at CERN -- the AWAKE experiment -- has been launched to study in detail the important physical processes and to demonstrate the power of proton-driven plasma wakefield acceleration. Here we review the physical principles and some experimental considerations for a future proton-driven plasma wakefield accelerator.

  14. Proton-driven plasma wakefield acceleration: a path to the future of high-energy particle physics

    International Nuclear Information System (INIS)

    Assmann, R; Gross, M; Bingham, R; Holloway, J; Bohl, T; Bracco, C; Butterworth, A; Feldbaumer, E; Goddard, B; Gschwendtner, E; Buttenschön, B; Caldwell, A; Chattopadhyay, S; Cipiccia, S; Jaroszynski, D; Fonseca, R A; Grulke, O; Kempkes, P; Huang, C; Jolly, S

    2014-01-01

    New acceleration technology is mandatory for the future elucidation of fundamental particles and their interactions. A promising approach is to exploit the properties of plasmas. Past research has focused on creating large-amplitude plasma waves by injecting an intense laser pulse or an electron bunch into the plasma. However, the maximum energy gain of electrons accelerated in a single plasma stage is limited by the energy of the driver. Proton bunches are the most promising drivers of wakefields to accelerate electrons to the TeV energy scale in a single stage. An experimental program at CERN—the AWAKE experiment—has been launched to study in detail the important physical processes and to demonstrate the power of proton-driven plasma wakefield acceleration. Here we review the physical principles and some experimental considerations for a future proton-driven plasma wakefield accelerator. (paper)

  15. Slipping slender bodies and enhanced flagellar locomotion

    Science.gov (United States)

    Man, Yi; Lauga, Eric

    2017-11-01

    In the biological world, many cells exploit slender appendages to swim, include numerous species of bacteria, algae and spermatozoa. A classical method to describe the flow field around such appendages is slender-body theory (SBT), which is often used to study flagellar motility in Newtonian fluids. However, biology environments are often rheologically complex due to the presence of polymers. These polymers generically phase-separate near rigid boundaries where low-viscosity fluid layers lead to effective slip on the surface. In this talk, we present an analytical derivation of SBT in the case where the no-slip boundary condition on the appendage is replaced by a Navier slip boundary condition. Our results demonstrate in particular a systematic reduction of the resistance coefficient of the slender filaments in their tangential direction, which leads to enhanced flagellar locomotion.

  16. Flagellar Motility of Trypanosoma cruzi Epimastigotes

    Directory of Open Access Journals (Sweden)

    G. Ballesteros-Rodea

    2012-01-01

    Full Text Available The hemoflagellate Trypanosoma cruzi is the causative agent of American trypanosomiasis. Despite the importance of motility in the parasite life cycle, little is known about T. cruzi motility, and there is no quantitative description of its flagellar beating. Using video microscopy and quantitative vectorial analysis of epimastigote trajectories, we find a forward parasite motility defined by tip-to-base symmetrical flagellar beats. This motion is occasionally interrupted by base-to-tip highly asymmetric beats, which represent the ciliary beat of trypanosomatid flagella. The switch between flagellar and ciliary beating facilitates the parasite's reorientation, which produces a large variability of movement and trajectories that results in different distance ranges traveled by the cells. An analysis of the distance, speed, and rotational angle indicates that epimastigote movement is not completely random, and the phenomenon is highly dependent on the parasite behavior and is characterized by directed and tumbling parasite motion as well as their combination, resulting in the alternation of rectilinear and intricate motility paths.

  17. Emergence of flagellar beating from the collective behavior of individual ATP-powered dyneins

    Science.gov (United States)

    Namdeo, S.; Onck, P. R.

    2016-10-01

    Flagella are hair-like projections from the surface of eukaryotic cells, and they play an important role in many cellular functions, such as cell-motility. The beating of flagella is enabled by their internal architecture, the axoneme, and is powered by a dense distribution of motor proteins, dyneins. The dyneins deliver the required mechanical work through the hydrolysis of ATP. Although the dynein-ATP cycle, the axoneme microstructure, and the flagellar-beating kinematics are well studied, their integration into a coherent picture of ATP-powered flagellar beating is still lacking. Here we show that a time-delayed negative-work-based switching mechanism is able to convert the individual sliding action of hundreds of dyneins into a regular overall beating pattern leading to propulsion. We developed a computational model based on a minimal representation of the axoneme consisting of two representative doublet microtubules connected by nexin links. The relative sliding of the microtubules is incorporated by modeling two groups of ATP-powered dyneins, each responsible for sliding in opposite directions. A time-delayed switching mechanism is postulated, which is key in converting the local individual sliding action of multiple dyneins into global beating. Our results demonstrate that an overall nonreciprocal beating pattern can emerge with time due to the spatial and temporal coordination of the individual dyneins. These findings provide insights in the fundamental working mechanism of axonemal dyneins and could possibly open new research directions in the field of flagellar motility.

  18. The Flagellar Regulon of Legionella—A Review

    Science.gov (United States)

    Appelt, Sandra; Heuner, Klaus

    2017-01-01

    The Legionella genus comprises more than 60 species. In particular, Legionella pneumophila is known to cause severe illnesses in humans. Legionellaceae are ubiquitous inhabitants of aquatic environments. Some Legionellaceae are motile and their motility is important to move around in habitats. Motility can be considered as a potential virulence factor as already shown for various human pathogens. The genes of the flagellar system, regulator and structural genes, are structured in hierarchical levels described as the flagellar regulon. Their expression is modulated by various environmental factors. For L. pneumophila it was shown that the expression of genes of the flagellar regulon is modulated by the actual growth phase and temperature. Especially, flagellated Legionella are known to express genes during the transmissive phase of growth that are involved in the expression of virulence traits. It has been demonstrated that the alternative sigma-28 factor is part of the link between virulence expression and motility. In the following review, the structure of the flagellar regulon of L. pneumophila is discussed and compared to other flagellar systems of different Legionella species. Recently, it has been described that Legionella micdadei and Legionella fallonii contain a second putative partial flagellar system. Hence, the report will focus on flagellated and non-flagellated Legionella strains, phylogenetic relationships, the role and function of the alternative sigma factor (FliA) and its anti-sigma-28 factor (FlgM). PMID:29104863

  19. Impact of fluorescent protein fusions on the bacterial flagellar motor

    NARCIS (Netherlands)

    Heo, M.; Nord, A. L.; Chamousset, D.; van Rijn, E.; Beaumont, H.J.E.; Pedaci, F.

    2017-01-01

    Fluorescent fusion proteins open a direct and unique window onto protein function. However, they also introduce the risk of perturbation of the function of the native protein. Successful applications of fluorescent fusions therefore rely on a careful assessment and minimization of the side

  20. Reactions of chicken sera to recombinant Campylobacter jejuni flagellar proteins.

    Science.gov (United States)

    Yeh, Hung-Yueh; Hiett, Kelli L; Line, John E

    2015-03-01

    Campylobacter jejuni is a Gram-negative spiral rod bacterium and is the leading but underreported bacterial food-borne pathogen that causes human campylobacteriosis worldwide. Raw or undercooked poultry products are regarded as a major source for human infection. C. jejuni flagella have been implicated in colonization and adhesion to the mucosal surface of chicken gastrointestinal tracts. Therefore, flagellar proteins would be the excellent targets for further investigation. In this report, we used the recombinant technology to generate a battery of C. jejuni flagellar proteins, which were purified by His tag affinity chromatography and determined antigenic profiles of these recombinant flagellar proteins using sera from chickens older than 6 weeks of age. The immunoblot results demonstrate that each chicken serum reacted to various numbers of recombinant flagellar proteins. Among these recombinant proteins, chicken sera reacted predominantly to the FlgE1, FlgK, FlhF, FliG and FliY proteins. These antibody screening results provide a rationale for further evaluation of these recombinant flagellar proteins as potential vaccines for chickens to improve food safety as well as investigation of host immune response to C. jejuni.

  1. In situ ellipsometric study of surface immobilization of flagellar filaments

    Energy Technology Data Exchange (ETDEWEB)

    Kurunczi, S., E-mail: kurunczi@mfa.kfki.hu [Department of Photonics, Research Institute for Technical Physics and Materials Science, H-1121, Konkoly Thege Miklos ut 29-33, Budapest (Hungary); Nemeth, A.; Huelber, T. [Department of Photonics, Research Institute for Technical Physics and Materials Science, H-1121, Konkoly Thege Miklos ut 29-33, Budapest (Hungary); Kozma, P. [Department of Photonics, Research Institute for Technical Physics and Materials Science, H-1121, Konkoly Thege Miklos ut 29-33, Budapest (Hungary); Department of Nanotechnology, Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, Veszprem, H-8200 (Hungary); Petrik, P. [Department of Photonics, Research Institute for Technical Physics and Materials Science, H-1121, Konkoly Thege Miklos ut 29-33, Budapest (Hungary); Jankovics, H. [Department of Nanotechnology, Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, Veszprem, H-8200 (Hungary); Sebestyen, A. [Department of Photonics, Research Institute for Technical Physics and Materials Science, H-1121, Konkoly Thege Miklos ut 29-33, Budapest (Hungary); Department of Nanotechnology, Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, Veszprem, H-8200 (Hungary); Vonderviszt, F. [Department of Photonics, Research Institute for Technical Physics and Materials Science, H-1121, Konkoly Thege Miklos ut 29-33, Budapest (Hungary); Department of Nanotechnology, Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, Veszprem, H-8200 (Hungary); Institute of Enzymology, Karolina ut 29-33, Budapest, H-1113 (Hungary); and others

    2010-10-15

    Protein filaments composed of thousands of subunits are promising candidates as sensing elements in biosensors. In this work in situ spectroscopic ellipsometry is applied to monitor the surface immobilization of flagellar filaments. This study is the first step towards the development of layers of filamentous receptors for sensor applications. Surface activation is performed using silanization and a subsequent glutaraldehyde crosslinking. Structure of the flagellar filament layers immobilized on activated and non-activated Si wafer substrates is determined using a two-layer effective medium model that accounted for the vertical density distribution of flagellar filaments with lengths of 300-1500 nm bound to the surface. The formation of the first interface layer can be explained by the multipoint covalent attachment of the filaments, while the second layer is mainly composed of tail pinned filaments floating upwards with the free parts. As confirmed by atomic force microscopy, covalent immobilization resulted in an increased surface density compared to absorption.

  2. Human sperm steer with second harmonics of the flagellar beat.

    Science.gov (United States)

    Saggiorato, Guglielmo; Alvarez, Luis; Jikeli, Jan F; Kaupp, U Benjamin; Gompper, Gerhard; Elgeti, Jens

    2017-11-10

    Sperm are propelled by bending waves traveling along their flagellum. For steering in gradients of sensory cues, sperm adjust the flagellar waveform. Symmetric and asymmetric waveforms result in straight and curved swimming paths, respectively. Two mechanisms causing spatially asymmetric waveforms have been proposed: an average flagellar curvature and buckling. We image flagella of human sperm tethered with the head to a surface. The waveform is characterized by a fundamental beat frequency and its second harmonic. The superposition of harmonics breaks the beat symmetry temporally rather than spatially. As a result, sperm rotate around the tethering point. The rotation velocity is determined by the second-harmonic amplitude and phase. Stimulation with the female sex hormone progesterone enhances the second-harmonic contribution and, thereby, modulates sperm rotation. Higher beat frequency components exist in other flagellated cells; therefore, this steering mechanism might be widespread and could inspire the design of synthetic microswimmers.

  3. Identification and characterization of a stage specific membrane protein involved in flagellar attachment in Trypanosoma brucei.

    Directory of Open Access Journals (Sweden)

    Katherine Woods

    Full Text Available Flagellar attachment is a visibly striking morphological feature of African trypanosomes but little is known about the requirements for attachment at a molecular level. This study characterizes a previously undescribed membrane protein, FLA3, which plays an essential role in flagellar attachment in Trypanosoma brucei. FLA3 is heavily N-glycosylated, locates to the flagellar attachment zone and appears to be a bloodstream stage specific protein. Ablation of the FLA3 mRNA rapidly led to flagellar detachment and a concomitant failure of cytokinesis in the long slender bloodstream form but had no effect on the procyclic form. Flagellar detachment was obvious shortly after induction of the dsRNA and the newly synthesized flagellum was often completely detached after it emerged from the flagellar pocket. Within 12 h most cells possessed detached flagella alongside the existing attached flagellum. These results suggest that proteins involved in attachment are not shared between the new and old attachment zones. In other respects the detached flagella appear normal, they beat rapidly although directional motion was lost, and they possess an apparently normal axoneme and paraflagellar rod structure. The flagellar attachment zone appeared to be disrupted when FLA3 was depleted. Thus, while flagellar attachment is a constitutive feature of the life cycle of trypanosomes, attachment requires stage specific elements at the protein level.

  4. Changes in the flagellar bundling time account for variations in swimming behavior of flagellated bacteria in viscous media

    Science.gov (United States)

    Qu, Zijie; Temel, Fatma; Henderikx, Rene; Breuer, Kenneth

    2017-11-01

    The motility of bacteria E.coli in viscous fluids has been widely studied, although conflicting results on the effect of viscosity on swimming speed abound. The swimming mode of wild-type E.coli is idealized as a run-and-tumble sequence in which periods of straight swimming at a constant speed are randomly interrupted by a tumble, defined as a sudden change of direction with a very low speed. Using a tracking microscope, we follow cells for extended time and find that the swimming behavior of a single cell can exhibit a variety of behaviors including run-and-tumble and ``slow-random-walk'' in which the cells move at relatively low speed without the characteristic run. Although the characteristic swimming speed varies between individuals and in different polymer solutions, we find that the skewness of the speed distribution is solely a function of viscosity, and uniquely determines the ratio of the average speed to the characteristic run speed. Using Resistive Force Theory and the cell-specific measured characteristic run speed, we show that differences in the swimming behavior observed in solutions of different viscosity are due to changes in the flagellar bundling time, which increases as the viscosity rises, due to lower rotation rate of the flagellar motor. National Science Foundation.

  5. Flagellar-phase variation: isolation of the rh1 gene

    International Nuclear Information System (INIS)

    Silverman, M.; Zieg, J.; Simon, M.

    1979-01-01

    In Salmonella, expression of flagellar antigen alternates between two serotypes (phases) encoded by two genes, H1 and H2. The mechanism which controls the alternative expression of the H1 and H2 genes was examined by cloning these genes and the genetic elements which control their activity on hybrid vehicles in Escherichia coli. H2 gene activity was shown to be controlled by a recombinational switch located adjacent to the H2 gene. Activity of the H1 gene is thought to be repressed, when the H2 gene is expressed, by the product of another gene, rhl (repressor of H1), which is controlled coordinately with the H2 gene. In this report, we describe the construction of hybrid lambda vehicles which contain, in addition to the H2 gene, a genetic activity corresponding to rhl. Variation of flagellar antigens analogous to that observed in Salmonella was observed when E. coli strains were transduced with the hybrid lambda. By using the lambda H2rhl hybrid to program protein syntheis in uv-irradiated cells, the synthesis of a polypeptide was correlated with rhl gene product activity. We conclude that the H2 region consists of two cotranscribed genes, H2 and rhl. The expression of both gene products is regulated by the same recombinational event

  6. Bacterial flagellar capping proteins adopt diverse oligomeric states

    Energy Technology Data Exchange (ETDEWEB)

    Postel, Sandra; Deredge, Daniel; Bonsor, Daniel A.; Yu, Xiong; Diederichs, Kay; Helmsing, Saskia; Vromen, Aviv; Friedler, Assaf; Hust, Michael; Egelman, Edward H.; Beckett, Dorothy; Wintrode, Patrick L.; Sundberg, Eric J. (UV); (Braunschweig); (Maryland-MED); (Konstanz); (Maryland); (Hebrew)

    2016-09-24

    Flagella are crucial for bacterial motility and pathogenesis. The flagellar capping protein (FliD) regulates filament assembly by chaperoning and sorting flagellin (FliC) proteins after they traverse the hollow filament and exit the growing flagellum tip. In the absence of FliD, flagella are not formed, resulting in impaired motility and infectivity. Here, we report the 2.2 Å resolution X-ray crystal structure of FliD fromPseudomonas aeruginosa, the first high-resolution structure of any FliD protein from any bacterium. Using this evidence in combination with a multitude of biophysical and functional analyses, we find thatPseudomonasFliD exhibits unexpected structural similarity to other flagellar proteins at the domain level, adopts a unique hexameric oligomeric state, and depends on flexible determinants for oligomerization. Considering that the flagellin filaments on which FliD oligomers are affixed vary in protofilament number between bacteria, our results suggest that FliD oligomer stoichiometries vary across bacteria to complement their filament assemblies.

  7. Flagellar motility confers epiphytic fitness advantages upon Pseudomonas syringae

    International Nuclear Information System (INIS)

    Haefele, D.M.; Lindow, S.E.

    1987-01-01

    The role of flagellar motility in determining the epiphytic fitness of an ice-nucleation-active strain of Pseudomonas syringae was examined. The loss of flagellar motility reduced the epiphytic fitness of a normally motile P. syringae strain as measured by its growth, survival, and competitive ability on bean leaf surfaces. Equal population sizes of motile parental or nonmotile mutant P. syringae strains were maintained on bean plants for at least 5 days following the inoculation of fully expanded primary leaves. However, when bean seedlings were inoculated before the primary leaves had expanded and bacterial populations on these leaves were quantified at full expansion, the population size of the nonmotile derivative strain reached only 0.9% that of either the motile parental or revertant strain. When fully expanded bean primary leaves were coinoculated with equal numbers of motile and nonmotile cells, the population size of a nonmotile derivative strain was one-third of that of the motile parental or revertant strain after 8 days. Motile and nonmotile cells were exposed in vitro and on plants to UV radiation and desiccating conditions. The motile and nonmotile strains exhibited equal resistance to both stresses in vitro. However, the population size of a nonmotile strain on leaves was less than 20% that of a motile revertant strain when sampled immediately after UV irradiation. Epiphytic populations of both motile and nonmotile P. syringae declined under desiccating conditions on plants, and after 8 days, the population size of a nonmotile strain was less than one-third that of the motile parental or revertant strain

  8. Rab23 is a flagellar protein in Trypanosoma brucei

    Directory of Open Access Journals (Sweden)

    Field Mark C

    2011-06-01

    Full Text Available Abstract Background Rab small GTPases are important mediators of membrane transport, and orthologues frequently retain similar locations and functions, even between highly divergent taxa. In metazoan organisms Rab23 is an important negative regulator of Sonic hedgehog signaling and is crucial for correct development and differentiation of cellular lineages by virtue of an involvement in ciliary recycling. Previously, we reported that Trypanosoma brucei Rab23 localized to the nuclear envelope 1, which is clearly inconsistent with the mammalian location and function. As T. brucei is unicellular the potential that Rab23 has no role in cell signaling was possible. Here we sought to further investigate the role(s of Rab23 in T. brucei to determine if Rab23 was an example of a Rab protein with divergent function in distinct taxa. Methods/major findings The taxonomic distribution of Rab23 was examined and compared with the presence of flagella/cilia in representative taxa. Despite evidence for considerable secondary loss, we found a clear correlation between a conventional flagellar structure and the presence of a Rab23 orthologue in the genome. By epitope-tagging, Rab23 was localized and found to be present at the flagellum throughout the cell cycle. However, RNAi knockdown did not result in a flagellar defect, suggesting that Rab23 is not required for construction or maintenance of the flagellum. Conclusions The location of Rab23 at the flagellum is conserved between mammals and trypanosomes and the Rab23 gene is restricted to flagellated organisms. These data may suggest the presence of a Rab23-mediated signaling mechanism in trypanosomes.

  9. Protein export through the bacterial flagellar type III export pathway.

    Science.gov (United States)

    Minamino, Tohru

    2014-08-01

    For construction of the bacterial flagellum, which is responsible for bacterial motility, the flagellar type III export apparatus utilizes both ATP and proton motive force across the cytoplasmic membrane and exports flagellar proteins from the cytoplasm to the distal end of the nascent structure. The export apparatus consists of a membrane-embedded export gate made of FlhA, FlhB, FliO, FliP, FliQ, and FliR and a water-soluble ATPase ring complex consisting of FliH, FliI, and FliJ. FlgN, FliS, and FliT act as substrate-specific chaperones that do not only protect their cognate substrates from degradation and aggregation in the cytoplasm but also efficiently transfer the substrates to the export apparatus. The ATPase ring complex facilitates the initial entry of the substrates into the narrow pore of the export gate. The export gate by itself is a proton-protein antiporter that uses the two components of proton motive force, the electric potential difference and the proton concentration difference, for different steps of the export process. A specific interaction of FlhA with FliJ located in the center of the ATPase ring complex allows the export gate to efficiently use proton motive force to drive protein export. The ATPase ring complex couples ATP binding and hydrolysis to its assembly-disassembly cycle for rapid and efficient protein export cycle. This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey. © 2013 Elsevier B.V. All rights reserved.

  10. Nonlinear instability in flagellar dynamics: a novel modulation mechanism in sperm migration?

    KAUST Repository

    Gadelha, H.; Gaffney, E. A.; Smith, D. J.; Kirkman-Brown, J. C.

    2010-01-01

    . We study the effect of geometrical nonlinearity, focusing on the spermatozoon flagellum. For a wide range of physiologically relevant parameters, the nonlinear model predicts that flagellar compression by the internal forces initiates an effective

  11. Minimum Information Loss Based Multi-kernel Learning for Flagellar Protein Recognition in Trypanosoma Brucei

    KAUST Repository

    Wang, Jim Jing-Yan; Gao, Xin

    2014-01-01

    for the purposes of both biological research and drug design. In this paper, we investigate computationally recognizing flagellar proteins in T. Brucei by pattern recognition methods. It is argued that an optimal decision function can be obtained as the difference

  12. Hierarchical protein export mechanism of the bacterial flagellar type III protein export apparatus.

    Science.gov (United States)

    Minamino, Tohru

    2018-06-01

    The bacterial flagellum is supramolecular motility machinery consisting of the basal body, the hook and the filament. Flagellar proteins are translocated across the cytoplasmic membrane via a type III protein export apparatus, diffuse down the central channel of the growing structure and assemble at the distal end. Flagellar assembly begins with the basal body, followed by the hook and finally the filament. The completion of hook assembly is the most important morphological checkpoint of the sequential flagellar assembly process. When the hook reaches its mature length of about 55 nm in Salmonella enterica, the type III protein export apparatus switches export specificity from proteins required for the structure and assembly of the hook to those responsible for filament assembly, thereby terminating hook assembly and initiating filament assembly. Three flagellar proteins, namely FliK, FlhB and FlhA, are responsible for this substrate specificity switching. Upon completion of the switching event, interactions among FlhA, the cytoplasmic ATPase complex and flagellar type III export chaperones establish the assembly order of the filament at the hook tip. Here, we describe our current understanding of a hierarchical protein export mechanism used in flagellar type III protein export.

  13. Cloning, overexpression, purification, crystallization and preliminary X-ray analysis of CheY3, a response regulator that directly interacts with the flagellar ‘switch complex’ in Vibrio cholerae

    International Nuclear Information System (INIS)

    Khamrui, Susmita; Biswas, Maitree; Sen, Udayaditya; Dasgupta, Jhimli

    2010-01-01

    A chemotaxis response regulator CheY3 from V. cholerae has been cloned, overexpressed, purified and crystallized. The crystals of CheY3 diffracted to 1.86 Å resolution. Vibrio cholerae is the aetiological agent of the severe diarrhoeal disease cholera. This highly motile organism uses the processes of motility and chemotaxis to travel and colonize the intestinal epithelium. Chemotaxis in V. cholerae is far more complex than that in Escherichia coli or Salmonella typhimurium, with multiple paralogues of various chemotaxis genes. In contrast to the single copy of the chemotaxis response-regulator protein CheY in E. coli, V. cholerae contains four CheYs (CheY1–CheY4), of which CheY3 is primarily responsible for interacting with the flagellar motor protein FliM, which is one of the major constituents of the ‘switch complex’ in the flagellar motor. This interaction is the key step that controls flagellar rotation in response to environmental stimuli. CheY3 has been cloned, overexpressed and purified by Ni–NTA affinity chromatography followed by gel filtration. Crystals of CheY3 were grown in space group R3, with a calculated Matthews coefficient of 2.33 Å 3 Da −1 (47% solvent content) assuming the presence of one molecule per asymmetric unit

  14. Flagellar region 3b supports strong expression of integrated DNA and the highest chromosomal integration efficiency of the Escherichia coli flagellar regions.

    Science.gov (United States)

    Juhas, Mario; Ajioka, James W

    2015-07-01

    The Gram-negative bacterium Escherichia coli is routinely used as the chassis for a variety of biotechnology and synthetic biology applications. Identification and analysis of reliable chromosomal integration and expression target loci is crucial for E. coli engineering. Chromosomal loci differ significantly in their ability to support integration and expression of the integrated genetic circuits. In this study, we investigate E. coli K12 MG1655 flagellar regions 2 and 3b. Integration of the genetic circuit into seven and nine highly conserved genes of the flagellar regions 2 (motA, motB, flhD, flhE, cheW, cheY and cheZ) and 3b (fliE, F, G, J, K, L, M, P, R), respectively, showed significant variation in their ability to support chromosomal integration and expression of the integrated genetic circuit. While not reducing the growth of the engineered strains, the integrations into all 16 target sites led to the loss of motility. In addition to high expression, the flagellar region 3b supports the highest efficiency of integration of all E. coli K12 MG1655 flagellar regions and is therefore potentially the most suitable for the integration of synthetic genetic circuits. © 2015 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

  15. Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella

    Directory of Open Access Journals (Sweden)

    Bin Dong

    2017-11-01

    Full Text Available Intraflagellar transport (IFT particles are composed of polyprotein complexes IFT-A and IFT-B as well as cargo adaptors such as the BBSome. Two IFT-B subunits, IFT25 and IFT27 were found to form a heterodimer, which is essential in exporting the BBSome out of the cilium but not involved in flagellar assembly and cytokinesis in vertebrates. Controversial results were, however, recorded to show that defects in IFT, flagellar assembly and even cytokinesis were caused by IFT27 knockdown in Chlamydomonas reinhardtii. Using C. reinhardtii as a model organism, we report that depletion of IFT25 has no effect on flagellar assembly and does not affect the entry of the BBSome into the flagellum, but IFT25 depletion did impair BBSome movement out of the flagellum, clarifying the evolutionally conserved role of IFT25 in regulating the exit of the BBSome from the flagellum cross species. Interestingly, depletion of IFT25 causes dramatic reduction of IFT27 as expected, which does not cause defects in flagellar assembly and cytokinesis in C. reinhardtii. Our data thus support that Chlamydomonas IFT27, like its vertebrate homologues, is not involved in flagellar assembly and cytokinesis.

  16. Architecture of the flagellar apparatus and related structures in the type species of Peridinium, P. cinctum (Dinophyceae)

    DEFF Research Database (Denmark)

    Calado, A.C.; Hansen, Gert; Moestrup, Øjvind

    1999-01-01

    The ultrastructure of Peridinium cinctum, was examined by serial sectioning with particular emphasis on the detailed construction of the flagellar apparatus. The pusular system of P. cinctum included two sac pusules in open connection with the flagellar canals; disorganized material was found ins...

  17. Role of calmodulin and calcineurin in regulating flagellar motility and wave polarity in Leishmania.

    Science.gov (United States)

    Mukhopadhyay, Aakash Gautam; Dey, Chinmoy Sankar

    2017-11-01

    We have previously reported the involvement of cyclic AMP in regulating flagellar waveforms in Leishmania. Here, we investigated the roles of calcium, calmodulin, and calcineurin in flagellar motility regulation in L. donovani. Using high-speed videomicroscopy, we show that calcium-independent calmodulin and calcineurin activity is necessary for motility in Leishmania. Inhibition of calmodulin and calcineurin induced ciliary beats interrupting flagellar beating in both live (in vivo) and ATP-reactivated (in vitro) parasites. Our results indicate that signaling mediated by calmodulin and calcineurin operates antagonistically to cAMP signaling in regulating the waveforms of Leishmania flagellum. These two pathways are possibly involved in maintaining the balance between the two waveforms, essential for responding to environmental cues, survival, and infectivity.

  18. Flagellar coordination in Chlamydomonas cells held on micropipettes.

    Science.gov (United States)

    Rüffer, U; Nultsch, W

    1998-01-01

    The two flagella of Chlamydomonas are known to beat synchronously: During breaststroke beating they are generally coordinated in a bilateral way while in shock responses during undulatory beating coordination is mostly parallel [Rüffer and Nultsch, 1995: Botanica Acta 108:169-276]. Analysis of a great number of shock responses revealed that in undulatory beats also periods of bilateral coordination are found and that the coordination type may change several times during a shock response, without concomitant changes of the beat envelope and the beat period. In normal wt cells no coordination changes are found during breaststroke beating, but only short temporary asynchronies: During 2 or 3 normal beats of the cis flagellum, the trans flagellum performs 3 or 4 flat beats with a reduced beat envelope and a smaller beat period, resulting in one additional trans beat. Long periods with flat beats of the same shape and beat period are found in both flagella of the non-phototactic mutant ptx1 and in defective wt 622E cells. During these periods, the coordination is parallel, the two flagella beat alternately. A correlation between normal asynchronous trans beats and the parallel-coordinated beats in the presumably cis defective cells and also the undulatory beats is discussed. In the cis defective cells, a perpetual spontaneous change between parallel beats with small beat periods (higher beat frequency) and bilateral beats with greater beat periods (lower beat frequency) are observed and render questionable the existence of two different intrinsic beat frequencies of the two flagella cis and trans. Asynchronies occur spontaneously but may also be induced by light changes, either step-up or step-down, but not by both stimuli in turn as breaststroke flagellar photoresponses (BFPRs). Asynchronies are not involved in phototaxis. They are independent of the BFPRs, which are supposed to be the basis of phototaxis. Both types of coordination must be assumed to be regulated

  19. In Vitro Reconstitution of Functional Type III Protein Export and Insights into Flagellar Assembly.

    Science.gov (United States)

    Terashima, Hiroyuki; Kawamoto, Akihiro; Tatsumi, Chinatsu; Namba, Keiichi; Minamino, Tohru; Imada, Katsumi

    2018-06-26

    The type III secretion system (T3SS) forms the functional core of injectisomes, protein transporters that allow bacteria to deliver virulence factors into their hosts for infection, and flagella, which are critical for many pathogens to reach the site of infection. In spite of intensive genetic and biochemical studies, the T3SS protein export mechanism remains unclear due to the difficulty of accurate measurement of protein export in vivo Here, we developed an in vitro flagellar T3S protein transport assay system using an inverted cytoplasmic membrane vesicle (IMV) for accurate and controlled measurements of flagellar protein export. We show that the flagellar T3SS in the IMV fully retains export activity. The flagellar hook was constructed inside the lumen of the IMV by adding purified component proteins externally to the IMV solution. We reproduced the hook length control and export specificity switch in the IMV consistent with that seen in the native cell. Previous in vivo analyses showed that flagellar protein export is driven by proton motive force (PMF) and facilitated by ATP hydrolysis by FliI, a T3SS-specific ATPase. Our in vitro assay recapitulated these previous in vivo observations but furthermore clearly demonstrated that even ATP hydrolysis by FliI alone can drive flagellar protein export. Moreover, this assay showed that addition of the FliH 2 /FliI complex to the assay solution at a concentration similar to that in the cell dramatically enhanced protein export, confirming that the FliH 2 /FliI complex in the cytoplasm is important for effective protein transport. IMPORTANCE The type III secretion system (T3SS) is the functional core of the injectisome, a bacterial protein transporter used to deliver virulence proteins into host cells, and bacterial flagella, critical for many pathogens. The molecular mechanism of protein transport is still unclear due to difficulties in accurate measurements of protein transport under well-controlled conditions in

  20. The Bacterial Flagellar Type III Export Gate Complex Is a Dual Fuel Engine That Can Use Both H+ and Na+ for Flagellar Protein Export.

    Directory of Open Access Journals (Sweden)

    Tohru Minamino

    2016-03-01

    Full Text Available The bacterial flagellar type III export apparatus utilizes ATP and proton motive force (PMF to transport flagellar proteins to the distal end of the growing flagellar structure for self-assembly. The transmembrane export gate complex is a H+-protein antiporter, of which activity is greatly augmented by an associated cytoplasmic ATPase complex. Here, we report that the export gate complex can use sodium motive force (SMF in addition to PMF across the cytoplasmic membrane to drive protein export. Protein export was considerably reduced in the absence of the ATPase complex and a pH gradient across the membrane, but Na+ increased it dramatically. Phenamil, a blocker of Na+ translocation, inhibited protein export. Overexpression of FlhA increased the intracellular Na+ concentration in the presence of 100 mM NaCl but not in its absence, suggesting that FlhA acts as a Na+ channel. In wild-type cells, however, neither Na+ nor phenamil affected protein export, indicating that the Na+ channel activity of FlhA is suppressed by the ATPase complex. We propose that the export gate by itself is a dual fuel engine that uses both PMF and SMF for protein export and that the ATPase complex switches this dual fuel engine into a PMF-driven export machinery to become much more robust against environmental changes in external pH and Na+ concentration.

  1. The Bacterial Flagellar Type III Export Gate Complex Is a Dual Fuel Engine That Can Use Both H+ and Na+ for Flagellar Protein Export.

    Science.gov (United States)

    Minamino, Tohru; Morimoto, Yusuke V; Hara, Noritaka; Aldridge, Phillip D; Namba, Keiichi

    2016-03-01

    The bacterial flagellar type III export apparatus utilizes ATP and proton motive force (PMF) to transport flagellar proteins to the distal end of the growing flagellar structure for self-assembly. The transmembrane export gate complex is a H+-protein antiporter, of which activity is greatly augmented by an associated cytoplasmic ATPase complex. Here, we report that the export gate complex can use sodium motive force (SMF) in addition to PMF across the cytoplasmic membrane to drive protein export. Protein export was considerably reduced in the absence of the ATPase complex and a pH gradient across the membrane, but Na+ increased it dramatically. Phenamil, a blocker of Na+ translocation, inhibited protein export. Overexpression of FlhA increased the intracellular Na+ concentration in the presence of 100 mM NaCl but not in its absence, suggesting that FlhA acts as a Na+ channel. In wild-type cells, however, neither Na+ nor phenamil affected protein export, indicating that the Na+ channel activity of FlhA is suppressed by the ATPase complex. We propose that the export gate by itself is a dual fuel engine that uses both PMF and SMF for protein export and that the ATPase complex switches this dual fuel engine into a PMF-driven export machinery to become much more robust against environmental changes in external pH and Na+ concentration.

  2. Minimum Information Loss Based Multi-kernel Learning for Flagellar Protein Recognition in Trypanosoma Brucei

    KAUST Repository

    Wang, Jim Jing-Yan

    2014-12-01

    Trypanosma brucei (T. Brucei) is an important pathogen agent of African trypanosomiasis. The flagellum is an essential and multifunctional organelle of T. Brucei, thus it is very important to recognize the flagellar proteins from T. Brucei proteins for the purposes of both biological research and drug design. In this paper, we investigate computationally recognizing flagellar proteins in T. Brucei by pattern recognition methods. It is argued that an optimal decision function can be obtained as the difference of probability functions of flagella protein and the non-flagellar protein for the purpose of flagella protein recognition. We propose to learn a multi-kernel classification function to approximate this optimal decision function, by minimizing the information loss of such approximation which is measured by the Kull back-Leibler (KL) divergence. An iterative multi-kernel classifier learning algorithm is developed to minimize the KL divergence for the problem of T. Brucei flagella protein recognition, experiments show its advantage over other T. Brucei flagellar protein recognition and multi-kernel learning methods. © 2014 IEEE.

  3. Self-Sustained Oscillatory Sliding Movement of Doublet Microtubules and Flagellar Bend Formation.

    Directory of Open Access Journals (Sweden)

    Sumio Ishijima

    Full Text Available It is well established that the basis for flagellar and ciliary movements is ATP-dependent sliding between adjacent doublet microtubules. However, the mechanism for converting microtubule sliding into flagellar and ciliary movements has long remained unresolved. The author has developed new sperm models that use bull spermatozoa divested of their plasma membrane and midpiece mitochondrial sheath by Triton X-100 and dithiothreitol. These models enable the observation of both the oscillatory sliding movement of activated doublet microtubules and flagellar bend formation in the presence of ATP. A long fiber of doublet microtubules extruded by synchronous sliding of the sperm flagella and a short fiber of doublet microtubules extruded by metachronal sliding exhibited spontaneous oscillatory movements and constructed a one beat cycle of flagellar bending by alternately actuating. The small sliding displacement generated by metachronal sliding formed helical bends, whereas the large displacement by synchronous sliding formed planar bends. Therefore, the resultant waveform is a half-funnel shape, which is similar to ciliary movements.

  4. Identification of flagellar motility genes in Yersinia ruckeri by transposon mutagenesis

    DEFF Research Database (Denmark)

    Evenhuis, Jason P:; LaPatra, Scott E.; Verner-Jeffreys, David W.

    2009-01-01

    Here we demonstrate that flagellar secretion is required for production of secreted lipase activity in the fish pathogen Yersinia ruckeri and that neither of these activities is necessary for virulence in rainbow trout. Our results suggest a possible mechanism for the emergence of nonmotile biotype...

  5. Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD

    DEFF Research Database (Denmark)

    Givskov, M; Eberl, L; Christiansen, Gunna

    1995-01-01

    . Expression of flagella is demonstrated to follow a growth-phase-dependent pattern. Cloning, complementation studies and DNA-sequencing analysis has identified a genetic region in Serratia liquefaciens which exhibits extensive homology to the Escherichia coli flhD flagellar master operon. Interruption...... of the chromosomal flhD operon in S. liquefaciens results in non-flagellated and phospholipase-negative cells, but the synthesis of other exoenzymes is not affected. By placing the flhD operon under the control of a foreign inducible promoter we have shown that increased transcription through the flhD operon leads...

  6. Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD

    DEFF Research Database (Denmark)

    Givskov, M; Eberl, L; Christiansen, Gunna

    1995-01-01

    When a liquid culture of Serratia spp. reaches the last part of the logarithmic phase of growth it induces the synthesis of several extracellular hydrolytic enzymes. In this communication we show that synthesis and secretion of the extracellular phospholipase is coupled to expression of flagella....... Expression of flagella is demonstrated to follow a growth-phase-dependent pattern. Cloning, complementation studies and DNA-sequencing analysis has identified a genetic region in Serratia liquefaciens which exhibits extensive homology to the Escherichia coli flhD flagellar master operon. Interruption...

  7. The phylogeny of swimming kinematics: The environment controls flagellar waveforms in sperm motility

    Science.gov (United States)

    Guasto, Jeffrey; Burton, Lisa; Zimmer, Richard; Hosoi, Anette; Stocker, Roman

    2013-11-01

    In recent years, phylogenetic and molecular analyses have dominated the study of ecology and evolution. However, physical interactions between organisms and their environment, a fundamental determinant of organism ecology and evolution, are mediated by organism form and function, highlighting the need to understand the mechanics of basic survival strategies, including locomotion. Focusing on spermatozoa, we combined high-speed video microscopy and singular value decomposition analysis to quantitatively compare the flagellar waveforms of eight species, ranging from marine invertebrates to humans. We found striking similarities in sperm swimming kinematics between genetically dissimilar organisms, which could not be uncovered by phylogenetic analysis. The emergence of dominant waveform patterns across species are suggestive of biological optimization for flagellar locomotion and point toward environmental cues as drivers of this convergence. These results reinforce the power of quantitative kinematic analysis to understand the physical drivers of evolution and as an approach to uncover new solutions for engineering applications, such as micro-robotics.

  8. Flagellar filament bio-templated inorganic oxide materials – towards an efficient lithium battery anode

    Science.gov (United States)

    Beznosov, Sergei N.; Veluri, Pavan S.; Pyatibratov, Mikhail G.; Chatterjee, Abhijit; MacFarlane, Douglas R.; Fedorov, Oleg V.; Mitra, Sagar

    2015-01-01

    Designing a new generation of energy-intensive and sustainable electrode materials for batteries to power a variety of applications is an imperative task. The use of biomaterials as a nanosized structural template for these materials has the potential to produce hitherto unachievable structures. In this report, we have used genetically modified flagellar filaments of the extremely halophilic archaea species Halobacterium salinarum to synthesize nanostructured iron oxide composites for use as a lithium-ion battery anode. The electrode demonstrated a superior electrochemical performance compared to existing literature results, with good capacity retention of 1032 mAh g−1 after 50 cycles and with high rate capability, delivering 770 mAh g−1 at 5 A g−1 (~5 C) discharge rate. This unique flagellar filament based template has the potential to provide access to other highly structured advanced energy materials in the future. PMID:25583370

  9. Flagellar filament bio-templated inorganic oxide materials - towards an efficient lithium battery anode.

    Science.gov (United States)

    Beznosov, Sergei N; Veluri, Pavan S; Pyatibratov, Mikhail G; Chatterjee, Abhijit; MacFarlane, Douglas R; Fedorov, Oleg V; Mitra, Sagar

    2015-01-13

    Designing a new generation of energy-intensive and sustainable electrode materials for batteries to power a variety of applications is an imperative task. The use of biomaterials as a nanosized structural template for these materials has the potential to produce hitherto unachievable structures. In this report, we have used genetically modified flagellar filaments of the extremely halophilic archaea species Halobacterium salinarum to synthesize nanostructured iron oxide composites for use as a lithium-ion battery anode. The electrode demonstrated a superior electrochemical performance compared to existing literature results, with good capacity retention of 1032 mAh g(-1) after 50 cycles and with high rate capability, delivering 770 mAh g(-1) at 5 A g(-1) (~5 C) discharge rate. This unique flagellar filament based template has the potential to provide access to other highly structured advanced energy materials in the future.

  10. Flagellar apparatus and nuclear chambers of the green dinoflagellate Gymnodinium chlorophorum

    DEFF Research Database (Denmark)

    Hansen, Gert; Moestrup, Øjvind

    2005-01-01

    The green dinoflagellate Gymnodinium chlorophorum (BAH ME 100, the type culture) was reexamined with emphasis on the structure of the flagellar apparatus and nuclear envelope. Like other Gymnodinium species, G. chlorophorum possessed a nuclear fibrous connective linking the flagellar apparatus...... present in G. chlorophorum similar to those reported in Gymnodinium aureolum and Gymnodinium nolleri. In contrast to the type species of Gymnodinium, Gymnodinium fuscum, only one nuclear pore was present per chamber. The presence of a feeding tube (peduncle) suggests that G. chlorophorum is mixotrophic....... Although the fine structure of G. chlorophorum revealed its affiliation to the Gymnodinium group the above discrepancies set it apart, indicating that it might belong in a different genus....

  11. Trypanin, a component of the flagellar Dynein regulatory complex, is essential in bloodstream form African trypanosomes.

    Directory of Open Access Journals (Sweden)

    Katherine S Ralston

    2006-09-01

    Full Text Available The Trypanosoma brucei flagellum is a multifunctional organelle with critical roles in motility, cellular morphogenesis, and cell division. Although motility is thought to be important throughout the trypanosome lifecycle, most studies of flagellum structure and function have been restricted to the procyclic lifecycle stage, and our knowledge of the bloodstream form flagellum is limited. We have previously shown that trypanin functions as part of a flagellar dynein regulatory system that transmits regulatory signals from the central pair apparatus and radial spokes to axonemal dyneins. Here we investigate the requirement for this dynein regulatory system in bloodstream form trypanosomes. We demonstrate that trypanin is localized to the flagellum of bloodstream form trypanosomes, in a pattern identical to that seen in procyclic cells. Surprisingly, trypanin RNA interference is lethal in the bloodstream form. These knockdown mutants fail to initiate cytokinesis, but undergo multiple rounds of organelle replication, accumulating multiple flagella, nuclei, kinetoplasts, mitochondria, and flagellum attachment zone structures. These findings suggest that normal flagellar beat is essential in bloodstream form trypanosomes and underscore the emerging concept that there is a dichotomy between trypanosome lifecycle stages with respect to factors that contribute to cell division and cell morphogenesis. This is the first time that a defined dynein regulatory complex has been shown to be essential in any organism and implicates the dynein regulatory complex and other enzymatic regulators of flagellar motility as candidate drug targets for the treatment of African sleeping sickness.

  12. Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model.

    Science.gov (United States)

    Engel, Benjamin D; Ludington, William B; Marshall, Wallace F

    2009-10-05

    The assembly and maintenance of eukaryotic flagella are regulated by intraflagellar transport (IFT), the bidirectional traffic of IFT particles (recently renamed IFT trains) within the flagellum. We previously proposed the balance-point length control model, which predicted that the frequency of train transport should decrease as a function of flagellar length, thus modulating the length-dependent flagellar assembly rate. However, this model was challenged by the differential interference contrast microscopy observation that IFT frequency is length independent. Using total internal reflection fluorescence microscopy to quantify protein traffic during the regeneration of Chlamydomonas reinhardtii flagella, we determined that anterograde IFT trains in short flagella are composed of more kinesin-associated protein and IFT27 proteins than trains in long flagella. This length-dependent remodeling of train size is consistent with the kinetics of flagellar regeneration and supports a revised balance-point model of flagellar length control in which the size of anterograde IFT trains tunes the rate of flagellar assembly.

  13. Tetrameric structure of the flagellar cap protein FliD from Serratia marcescens.

    Science.gov (United States)

    Cho, So Yeon; Song, Wan Seok; Hong, Ho Jeong; Lee, Geun-Shik; Kang, Seung Goo; Ko, Hyun-Jeong; Kim, Pyeung-Hyeun; Yoon, Sung-Il

    2017-07-15

    Bacterial motility is provided by the flagellum. FliD is located at the distal end of the flagellum and plays a key role in the insertion of each flagellin protein at the growing tip of the flagellar filament. Because FliD functions as an oligomer, the determination of the oligomeric state of FliD is critical to understanding the molecular mechanism of FliD-mediated flagellar growth. FliD has been shown to adopt a pentameric or a hexameric structure depending on the bacterial species. Here, we report another distinct oligomeric form of FliD based on structural and biochemical studies. The crystal structures of the D2 and D3 domains of Serratia marcescens FliD (smFliD) were determined in two crystal forms and together revealed that smFliD assembles into a tetrameric architecture that resembles a four-pointed star plate. smFliD tetramerization was also confirmed in solution by cross-linking experiments. Although smFliD oligomerizes in a head-to-tail orientation using a common primary binding interface between the D2 and D3' domains (the prime denotes the second subunit in the oligomer) similarly to other FliD orthologs, the smFliD tetramer diverges to present a unique secondary D2-D2' binding interface. Our structure-based comparative analysis of FliD suggests that bacteria have developed diverse species-specific oligomeric forms of FliD that range from tetramers to hexamers for flagellar growth. Copyright © 2017 Elsevier Inc. All rights reserved.

  14. Nonlinear instability in flagellar dynamics: a novel modulation mechanism in sperm migration?

    KAUST Repository

    Gadelha, H.

    2010-05-12

    Throughout biology, cells and organisms use flagella and cilia to propel fluid and achieve motility. The beating of these organelles, and the corresponding ability to sense, respond to and modulate this beat is central to many processes in health and disease. While the mechanics of flagellum-fluid interaction has been the subject of extensive mathematical studies, these models have been restricted to being geometrically linear or weakly nonlinear, despite the high curvatures observed physiologically. We study the effect of geometrical nonlinearity, focusing on the spermatozoon flagellum. For a wide range of physiologically relevant parameters, the nonlinear model predicts that flagellar compression by the internal forces initiates an effective buckling behaviour, leading to a symmetry-breaking bifurcation that causes profound and complicated changes in the waveform and swimming trajectory, as well as the breakdown of the linear theory. The emergent waveform also induces curved swimming in an otherwise symmetric system, with the swimming trajectory being sensitive to head shape-no signalling or asymmetric forces are required. We conclude that nonlinear models are essential in understanding the flagellar waveform in migratory human sperm; these models will also be invaluable in understanding motile flagella and cilia in other systems.

  15. Autonomously responsive pumping by a bacterial flagellar forest: A mean-field approach

    Science.gov (United States)

    Martindale, James D.; Fu, Henry C.

    2017-09-01

    This study is motivated by a microfluidic device that imparts a magnetic torque on an array of bacterial flagella. Bacterial flagella can transform their helical geometry autonomously in response to properties of the background fluid, which provides an intriguing mechanism allowing their use as an engineered element for the regulation or transport of chemicals in microscale applications. The synchronization of flagellar phase has been widely studied in biological contexts, but here we examine the synchronization of flagellar tilt, which is necessary for effective pumping. We first examine the effects of helical geometry and tilt on the pumping flows generated by a single rotating flagellum. Next, we explore a mean-field model for an array of helical flagella to understand how collective tilt arises and influences pumping. The mean-field methodology allows us to take into account possible phase differences through a time-averaging procedure and to model an infinite array of flagella. We find array separation distances, magnetic field strengths, and rotation frequencies that produce nontrivial self-consistent pumping solutions. For individual flagella, pumping is reversed when helicity or rotation is reversed; in contrast, when collective effects are included, self-consistent tilted pumping solutions become untilted nonpumping solutions when helicity or rotation is reversed.

  16. Ultrastructure of the harmful unarmored dinoflagellate Cochlodinium polykrikoides (Dinophyceae) with reference to the apical groove and Flagellar apparatus.

    Science.gov (United States)

    Iwataki, Mitsunori; Hansen, Gert; Moestrup, Øjvind; Matsuoka, Kazumi

    2010-01-01

    The external and internal ultrastructure of the harmful unarmored dinoflagellate Cochlodinium polykrikoides Margalef has been examined with special reference to the apical groove and three-dimensional structure of the flagellar apparatus. The apical groove is U-shaped and connected to the anterior sulcal extension on the dorsal side of the epicone. The eyespot is located dorsally and composed of two layers of globules situated within the chloroplast. A narrow invagination of the plasma membrane is associated with the eyespot. The nuclear envelope has normal nuclear pores similar to other eukaryotes but different from the Gymnodinium group with diagnostic nuclear chambers. The longitudinal and transverse basal bodies are separated by approximately 0.5-1.0 microm and interconnected directly by a striated basal body connective and indirectly by microtubular and fibrous structures. Characteristic features of the flagellar apparatus are as follows: (1) a nuclear extension projects to the R1 (longitudinal microtubular root) and is connected to the root by thin fibrous material; (2) fibrillar structures are associated with the longitudinal and transverse flagellar canal; and (3) a striated ventral connective extends toward the posterior end of the cell along the longitudinal flagellar canal. We conclude, based on both morphological and molecular evidence, that Cochlodinium is only distantly related to Gymnodinium.

  17. Assembly and stoichiometry of the core structure of the bacterial flagellar type III export gate complex.

    Science.gov (United States)

    Fukumura, Takuma; Makino, Fumiaki; Dietsche, Tobias; Kinoshita, Miki; Kato, Takayuki; Wagner, Samuel; Namba, Keiichi; Imada, Katsumi; Minamino, Tohru

    2017-08-01

    The bacterial flagellar type III export apparatus, which is required for flagellar assembly beyond the cell membranes, consists of a transmembrane export gate complex and a cytoplasmic ATPase complex. FlhA, FlhB, FliP, FliQ, and FliR form the gate complex inside the basal body MS ring, although FliO is required for efficient export gate formation in Salmonella enterica. However, it remains unknown how they form the gate complex. Here we report that FliP forms a homohexameric ring with a diameter of 10 nm. Alanine substitutions of conserved Phe-137, Phe-150, and Glu-178 residues in the periplasmic domain of FliP (FliPP) inhibited FliP6 ring formation, suppressing flagellar protein export. FliO formed a 5-nm ring structure with 3 clamp-like structures that bind to the FliP6 ring. The crystal structure of FliPP derived from Thermotoga maritia, and structure-based photo-crosslinking experiments revealed that Phe-150 and Ser-156 of FliPP are involved in the FliP-FliP interactions and that Phe-150, Arg-152, Ser-156, and Pro-158 are responsible for the FliP-FliO interactions. Overexpression of FliP restored motility of a ∆fliO mutant to the wild-type level, suggesting that the FliP6 ring is a functional unit in the export gate complex and that FliO is not part of the final gate structure. Copurification assays revealed that FlhA, FlhB, FliQ, and FliR are associated with the FliO/FliP complex. We propose that the assembly of the export gate complex begins with FliP6 ring formation with the help of the FliO scaffold, followed by FliQ, FliR, and FlhB and finally FlhA during MS ring formation.

  18. The Lipid Raft Proteome of African Trypanosomes Contains Many Flagellar Proteins.

    Science.gov (United States)

    Sharma, Aabha I; Olson, Cheryl L; Engman, David M

    2017-08-24

    Lipid rafts are liquid-ordered membrane microdomains that form by preferential association of 3-β-hydroxysterols, sphingolipids and raft-associated proteins often having acyl modifications. We isolated lipid rafts of the protozoan parasite Trypanosoma brucei and determined the protein composition of lipid rafts in the cell. This analysis revealed a striking enrichment of flagellar proteins and several putative signaling proteins in the lipid raft proteome. Calpains and intraflagellar transport proteins, in particular, were found to be abundant in the lipid raft proteome. These findings provide additional evidence supporting the notion that the eukaryotic cilium/flagellum is a lipid raft-enriched specialized structure with high concentrations of sterols, sphingolipids and palmitoylated proteins involved in environmental sensing and cell signaling.

  19. Testing the time-of-flight model for flagellar length sensing.

    Science.gov (United States)

    Ishikawa, Hiroaki; Marshall, Wallace F

    2017-11-07

    Cilia and flagella are microtubule-based organelles that protrude from the surface of most cells, are important to the sensing of extracellular signals, and make a driving force for fluid flow. Maintenance of flagellar length requires an active transport process known as intraflagellar transport (IFT). Recent studies reveal that the amount of IFT injection negatively correlates with the length of flagella. These observations suggest that a length-dependent feedback regulates IFT. However, it is unknown how cells recognize the length of flagella and control IFT. Several theoretical models try to explain this feedback system. We focused on one of the models, the "time-of-flight" model, which measures the length of flagella on the basis of the travel time of IFT protein in the flagellar compartment. We tested the time-of-flight model using Chlamydomonas dynein mutant cells, which show slower retrograde transport speed. The amount of IFT injection in dynein mutant cells was higher than that in control cells. This observation does not support the prediction of the time-of-flight model and suggests that Chlamydomonas uses another length-control feedback system rather than that described by the time-of-flight model. © 2017 Ishikawa and Marshall. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

  20. Evidence for loss of a partial flagellar glycolytic pathway during trypanosomatid evolution.

    Directory of Open Access Journals (Sweden)

    Robert W B Brown

    Full Text Available Classically viewed as a cytosolic pathway, glycolysis is increasingly recognized as a metabolic pathway exhibiting surprisingly wide-ranging variations in compartmentalization within eukaryotic cells. Trypanosomatid parasites provide an extreme view of glycolytic enzyme compartmentalization as several glycolytic enzymes are found exclusively in peroxisomes. Here, we characterize Trypanosoma brucei flagellar proteins resembling glyceraldehyde-3-phosphate dehydrogenase (GAPDH and phosphoglycerate kinase (PGK: we show the latter associates with the axoneme and the former is a novel paraflagellar rod component. The paraflagellar rod is an essential extra-axonemal structure in trypanosomes and related protists, providing a platform into which metabolic activities can be built. Yet, bioinformatics interrogation and structural modelling indicate neither the trypanosome PGK-like nor the GAPDH-like protein is catalytically active. Orthologs are present in a free-living ancestor of the trypanosomatids, Bodo saltans: the PGK-like protein from B. saltans also lacks key catalytic residues, but its GAPDH-like protein is predicted to be catalytically competent. We discuss the likelihood that the trypanosome GAPDH-like and PGK-like proteins constitute molecular evidence for evolutionary loss of a flagellar glycolytic pathway, either as a consequence of niche adaptation or the re-localization of glycolytic enzymes to peroxisomes and the extensive changes to glycolytic flux regulation that accompanied this re-localization. Evidence indicating loss of localized ATP provision via glycolytic enzymes therefore provides a novel contribution to an emerging theme of hidden diversity with respect to compartmentalization of the ubiquitous glycolytic pathway in eukaryotes. A possibility that trypanosome GAPDH-like protein additionally represents a degenerate example of a moonlighting protein is also discussed.

  1. Porters versus rowers: a unified stochastic model of motor proteins.

    Science.gov (United States)

    Leibler, S; Huse, D A

    1993-06-01

    We present a general phenomenological theory for chemical to mechanical energy transduction by motor enzymes which is based on the classical "tight-coupling" mechanism. The associated minimal stochastic model takes explicitly into account both ATP hydrolysis and thermal noise effects. It provides expressions for the hydrolysis rate and the sliding velocity, as functions of the ATP concentration and the number of motor enzymes. It explains in a unified way many results of recent in vitro motility assays. More importantly, the theory provides a natural classification scheme for the motors: it correlates the biochemical and mechanical differences between "porters" such as cellular kinesins or dyneins, and "rowers" such as muscular myosins or flagellar dyneins.

  2. CRIS-a novel cAMP-binding protein controlling spermiogenesis and the development of flagellar bending.

    Directory of Open Access Journals (Sweden)

    Anke Miriam Krähling

    Full Text Available The second messengers cAMP and cGMP activate their target proteins by binding to a conserved cyclic nucleotide-binding domain (CNBD. Here, we identify and characterize an entirely novel CNBD-containing protein called CRIS (cyclic nucleotide receptor involved in sperm function that is unrelated to any of the other members of this protein family. CRIS is exclusively expressed in sperm precursor cells. Cris-deficient male mice are either infertile due to a lack of sperm resulting from spermatogenic arrest, or subfertile due to impaired sperm motility. The motility defect is caused by altered Ca(2+ regulation of flagellar beat asymmetry, leading to a beating pattern that is reminiscent of sperm hyperactivation. Our results suggest that CRIS interacts during spermiogenesis with Ca(2+-regulated proteins that--in mature sperm--are involved in flagellar bending.

  3. Motor Neurons

    DEFF Research Database (Denmark)

    Hounsgaard, Jorn

    2017-01-01

    Motor neurons translate synaptic input from widely distributed premotor networks into patterns of action potentials that orchestrate motor unit force and motor behavior. Intercalated between the CNS and muscles, motor neurons add to and adjust the final motor command. The identity and functional...... in in vitro preparations is far from complete. Nevertheless, a foundation has been provided for pursuing functional significance of intrinsic response properties in motoneurons in vivo during motor behavior at levels from molecules to systems....

  4. Extensive flagellar remodeling during the complex life cycle of &ITParatrypanosoma&IT, an early-branching trypanosomatid

    Czech Academy of Sciences Publication Activity Database

    Skalický, Tomáš; Dobáková, Eva; Wheeler, R. J.; Tesařová, Martina; Flegontov, P.; Jirsová, Dagmar; Votýpka, Jan; Yurchenko, V.; Ayala, F. J.; Lukeš, Julius

    2017-01-01

    Roč. 114, č. 44 (2017), s. 11757-11762 ISSN 0027-8424 R&D Projects: GA ČR(CZ) GA14-23986S; GA MŠk LL1601; GA MŠk(CZ) LM2015062 Institutional support: RVO:60077344 Keywords : trypanosomatid * evolution * flagellar remodeling * haptomonads * cytostome Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology Impact factor: 9.661, year: 2016

  5. Deduction of upstream sequences of Xanthomonas campestris flagellar genes responding to transcription activation by FleQ

    International Nuclear Information System (INIS)

    Hu, R.-M.; Yang, T.-C.; Yang, S.-H.; Tseng, Y.-H.

    2005-01-01

    Xanthomonas campestris pv. campestris (Xcc), a close relative to Pseudomonas aeruginosa, is the pathogen causing black rot in cruciferous plants. In P. aeruginosa, FleQ serves as a cognate activator of σ 54 in transcription from several σ 54 -dependent promoters of flagellar genes. These P. aeruginosa promoters have been analyzed for FleQ-binding sequences; however, no consensus was deduced. Xcc, although lacks fleSR, has a fleQ homologue residing among over 40 contiguously clustered flagellar genes. A fleQ mutant, Xc17fleQ, constructed by insertional mutation is deficient in FleQ protein, non-flagellated, and immobile. Transcriptional fusion assays on six putative σ 54 -dependent promoters of the flagellar genes, fliE, fliQ, fliL, flgG, flgB, and flhF, indicated that each of them is also FleQ dependent. Each of these promoters has a sequence with weak consensus to 5'-gaaacCCgccgCcgctTt-3', immediately upstream of the predicted σ 54 -binding site, with an imperfect inverted repeat containing a GC-rich center flanked by several A and T at 5'- and 3'-ends, respectively. Replacing this region in fliE promoter with a HindIII recognition sequence abolished the transcription, indicating that this region responds to transcription activation by FleQ

  6. Association of Lis1 with outer arm dynein is modulated in response to alterations in flagellar motility

    Science.gov (United States)

    Rompolas, Panteleimon; Patel-King, Ramila S.; King, Stephen M.

    2012-01-01

    The cytoplasmic dynein regulatory factor Lis1, which induces a persistent tight binding to microtubules and allows for transport of cargoes under high-load conditions, is also present in motile cilia/flagella. We observed that Lis1 levels in flagella of Chlamydomonas strains that exhibit defective motility due to mutation of various axonemal substructures were greatly enhanced compared with wild type; this increase was absolutely dependent on the presence within the flagellum of the outer arm dynein α heavy chain/light chain 5 thioredoxin unit. To assess whether cells might interpret defective motility as a “high-load environment,” we reduced the flagellar beat frequency of wild-type cells through enhanced viscous load and by reductive stress; both treatments resulted in increased levels of flagellar Lis1, which altered the intrinsic beat frequency of the trans flagellum. Differential extraction of Lis1 from wild-type and mutant axonemes suggests that the affinity of outer arm dynein for Lis1 is directly modulated. In cytoplasm, Lis1 localized to two punctate structures, one of which was located near the base of the flagella. These data reveal that the cell actively monitors motility and dynamically modulates flagellar levels of the dynein regulatory factor Lis1 in response to imposed alterations in beat parameters. PMID:22855525

  7. The MogR Transcriptional Repressor Regulates Nonhierarchal Expression of Flagellar Motility Genes and Virulence in Listeria monocytogenes.

    Directory of Open Access Journals (Sweden)

    2006-04-01

    Full Text Available Flagella are surface structures critical for motility and virulence of many bacterial species. In Listeria monocytogenes, MogR tightly represses expression of flagellin (FlaA during extracellular growth at 37 degrees C and during intracellular infection. MogR is also required for full virulence in a murine model of infection. Using in vitro and in vivo infection models, we determined that the severe virulence defect of MogR-negative bacteria is due to overexpression of FlaA. Specifically, overproduction of FlaA in MogR-negative bacteria caused pleiotropic defects in bacterial division (chaining phenotype, intracellular spread, and virulence in mice. DNA binding and microarray analyses revealed that MogR represses transcription of all known flagellar motility genes by binding directly to a minimum of two TTTT-N(5-AAAA recognition sites positioned within promoter regions such that RNA polymerase binding is occluded. Analysis of MogR protein levels demonstrated that modulation of MogR repression activity confers the temperature-specificity to flagellar motility gene expression. Epistasis analysis revealed that MogR repression of transcription is antagonized in a temperature-dependent manner by the DegU response regulator and that DegU further regulates FlaA levels through a posttranscriptional mechanism. These studies provide the first known example to our knowledge of a transcriptional repressor functioning as a master regulator controlling nonhierarchal expression of flagellar motility genes.

  8. Genetical and functional investigation of fliC genes encoding flagellar serotype H4 in wildtype strains of Escherichia coli and in a laboratory E. coli K-12 strain expressing flagellar antigen type H48

    Directory of Open Access Journals (Sweden)

    Schaudinn Christoph

    2005-01-01

    Full Text Available Abstract Background Serotyping of O-(lipopolysaccharide and H-(flagellar antigens is a wideley used method for identification of pathogenic strains and clones of Escherichia coli. At present, 176 O- and 53 H-antigens are described for E. coli which occur in different combinations in the strains. The flagellar antigen H4 is widely present in E. coli strains of different O-serotypes and pathotypes and we have investigated the genetic relationship between H4 encoding fliC genes by PCR, nucleotide sequencing and expression studies. Results The complete nucleotide sequence of fliC genes present in E. coli reference strains U9-41 (O2:K1:H4 and P12b (O15:H17 was determined and both were found 99.3% (1043 of 1050 nucleotides identical in their coding sequence. A PCR/RFLP protocol was developed for typing of fliC-H4 strains and 88 E. coli strains reacting with H4 antiserum were investigated. Nucleotide sequencing of complete fliC genes of six E. coli strains which were selected based on serum agglutination titers, fliC-PCR genotyping and reference data revealed 96.6 to 100% identity on the amino acid level. The functional expression of flagellin encoded by fliC-H4 from strain U9-41 and from our strain P12b which is an H4 expressing variant type was investigated in the E. coli K-12 strain JM109 which encodes flagellar type H48. The fliC recombinant plasmid carrying JM109 strains reacted with both H4 and H48 specific antisera whereas JM109 reacted only with the H48 antiserum. By immunoelectron microscopy, we could show that the flagella made by the fliC-H4 recombinant plasmid carrying strain are constituted of H48 and H4 flagellins which are co-assembled into functional flagella. Conclusion The flagellar serotype H4 is encoded by closely related fliC genes present in serologically different types of E. coli strainswhich were isolated at different time periods and geographical locations. Our expression studies show for the first time, that flagellins of

  9. CDKL5 regulates flagellar length and localizes to the base of the flagella in Chlamydomonas

    Science.gov (United States)

    Tam, Lai-Wa; Ranum, Paul T.; Lefebvre, Paul A.

    2013-01-01

    The length of Chlamydomonas flagella is tightly regulated. Mutations in four genes—LF1, LF2, LF3, and LF4—cause cells to assemble flagella up to three times wild-type length. LF2 and LF4 encode protein kinases. Here we describe a new gene, LF5, in which null mutations cause cells to assemble flagella of excess length. The LF5 gene encodes a protein kinase very similar in sequence to the protein kinase CDKL5. In humans, mutations in this kinase cause a severe form of juvenile epilepsy. The LF5 protein localizes to a unique location: the proximal 1 μm of the flagella. The proximal localization of the LF5 protein is lost when genes that make up the proteins in the cytoplasmic length regulatory complex (LRC)—LF1, LF2, and LF3—are mutated. In these mutants LF5p becomes localized either at the distal tip of the flagella or along the flagellar length, indicating that length regulation involves, at least in part, control of LF5p localization by the LRC. PMID:23283985

  10. Imaging of the 3D dynamics of flagellar beating in human sperm.

    Science.gov (United States)

    Silva-Villalobos, F; Pimentel, J A; Darszon, A; Corkidi, G

    2014-01-01

    The study of the mechanical and environmental factors that regulate a fundamental event such as fertilization have been subject of multiple studies. Nevertheless, the microscopical size of the spermatozoa and the high beating frequency of their flagella (up to 20 Hz) impose a series of technological challenges for the study of the mechanical factors implicated. Traditionally, due to the inherent characteristics of the rapid sperm movement, and to the technological limitations of microscopes (optical or confocal) to follow in three dimensions (3D) their movement, the analysis of their dynamics has been studied in two dimensions, when the head is confined to a surface. Flagella propel sperm and while their head can be confined to a surface, flagellar movement is not restricted to 2D, always displaying 3D components. In this work, we present a highly novel and useful tool to analyze sperm flagella dynamics in 3D. The basis of the method is a 100 Hz oscillating objective mounted on a bright field optical microscope covering a 16 microns depth space at a rate of ~ 5000 images per second. The best flagellum focused subregions were associated to their respective Z real 3D position. Unprecedented graphical results making evident the 3D movement of the flagella are shown in this work and supplemental material illustrating a 3D animation using the obtained experimental results is also included.

  11. Crystallization of FcpA from Leptospira, a novel flagellar protein that is essential for pathogenesis.

    Science.gov (United States)

    San Martin, Fabiana; Mechaly, Ariel E; Larrieux, Nicole; Wunder, Elsio A; Ko, Albert I; Picardeau, Mathieu; Trajtenberg, Felipe; Buschiazzo, Alejandro

    2017-03-01

    The protein FcpA is a unique component of the flagellar filament of spirochete bacteria belonging to the genus Leptospira. Although it plays an essential role in translational motility and pathogenicity, no structures of FcpA homologues are currently available in the PDB. Its three-dimensional structure will unveil the novel motility mechanisms that render pathogenic Leptospira particularly efficient at invading and disseminating within their hosts, causing leptospirosis in humans and animals. FcpA from L. interrogans was purified and crystallized, but despite laborious attempts no useful X ray diffraction data could be obtained. This challenge was solved by expressing a close orthologue from the related saprophytic species L. biflexa. Three different crystal forms were obtained: a primitive and a centred monoclinic form, as well as a hexagonal variant. All forms diffracted X-rays to suitable resolutions for crystallographic analyses, with the hexagonal type typically reaching the highest limits of 2.0 Å and better. A variation of the quick-soaking procedure resulted in an iodide derivative that was instrumental for single-wavelength anomalous diffraction methods.

  12. Are there intracellular Ca2+ oscillations correlated with flagellar beating in human sperm? A three vs. two-dimensional analysis.

    Science.gov (United States)

    Corkidi, G; Montoya, F; Hernández-Herrera, P; Ríos-Herrera, W A; Müller, M F; Treviño, C L; Darszon, A

    2017-09-01

    Are there intracellular Ca2+ ([Ca2+]i) oscillations correlated with flagellar beating in human sperm? The results reveal statistically significant [Ca2+]i oscillations that are correlated with the human sperm flagellar beating frequency, when measured in three-dimensions (3D). Fast [Ca2+]i oscillations that are correlated to the beating flagellar frequency of cells swimming in a restricted volume have been detected in hamster sperm. To date, such findings have not been confirmed in any other mammalian sperm species. An important question that has remained regarding these observations is whether the fast [Ca2+]i oscillations are real or might they be due to remaining defocusing effects of the Z component arising from the 3D beating of the flagella. Healthy donors whose semen samples fulfill the WHO criteria between the age of 18-28 were selected. Cells from at least six different donors were utilized for analysis. Approximately the same number of experimental and control cells were analyzed. Motile cells were obtained by the swim-up technique and were loaded with Fluo-4 (Ca2+ sensitive dye) or with Calcein (Ca2+ insensitive dye). Ni2+ was used as a non-specific plasma membrane Ca2+ channel blocker. Fluorescence data and flagella position were acquired in 3D. Each cell was recorded for up to 5.6 s within a depth of 16 microns with a high speed camera (coupled to an image intensifier) acquiring at a rate of 3000 frames per second, while an oscillating objective vibrated at 90 Hz via a piezoelectric device. From these samples, eight experimental and nine control sperm cells were analyzed in both 2D and 3D. We have implemented a new system that allows [Ca2+]i measurements of the human sperm flagellum beating in 3D. These measurements reveal statistically significant [Ca2+]i oscillations that correlate with the flagellar beating frequency. These oscillations may arise from intracellular sources and/or Ca2+ transporters, as they were insensitive to external Ni2+, a non

  13. Characterization of a subunit of the outer dynein arm docking complex necessary for correct flagellar assembly in Leishmania donovani.

    Directory of Open Access Journals (Sweden)

    Simone Harder

    Full Text Available BACKGROUND: In order to proceed through their life cycle, Leishmania parasites switch between sandflies and mammals. The flagellated promastigote cells transmitted by the insect vector are phagocytized by macrophages within the mammalian host and convert into the amastigote stage, which possesses a rudimentary flagellum only. During an earlier proteomic study of the stage differentiation of the parasite we identified a component of the outer dynein arm docking complex, a structure of the flagellar axoneme. The 70 kDa subunit of the outer dynein arm docking complex consists of three subunits altogether and is essential for the assembly of the outer dynein arm onto the doublet microtubule of the flagella. According to the nomenclature of the well-studied Chlamydomonas reinhardtii complex we named the Leishmania protein LdDC2. METHODOLOGY/PRINCIPAL FINDINGS: This study features a characterization of the protein over the life cycle of the parasite. It is synthesized exclusively in the promastigote stage and localizes to the flagellum. Gene replacement mutants of lddc2 show reduced growth rates and diminished flagellar length. Additionally, the normally spindle-shaped promastigote parasites reveal a more spherical cell shape giving them an amastigote-like appearance. The mutants lose their motility and wiggle in place. Ultrastructural analyses reveal that the outer dynein arm is missing. Furthermore, expression of the amastigote-specific A2 gene family was detected in the deletion mutants in the absence of a stage conversion stimulus. In vitro infectivity is slightly increased in the mutant cell line compared to wild-type Leishmania donovani parasites. CONCLUSIONS/SIGNIFICANCE: Our results indicate that the correct assembly of the flagellum has a great influence on the investigated characteristics of Leishmania parasites. The lack of a single flagellar protein causes an aberrant morphology, impaired growth and altered infectiousness of the parasite.

  14. Independent evolution of neurotoxin and flagellar genetic loci in proteolytic Clostridium botulinum.

    Science.gov (United States)

    Carter, Andrew T; Paul, Catherine J; Mason, David R; Twine, Susan M; Alston, Mark J; Logan, Susan M; Austin, John W; Peck, Michael W

    2009-03-19

    Proteolytic Clostridium botulinum is the causative agent of botulism, a severe neuroparalytic illness. Given the severity of botulism, surprisingly little is known of the population structure, biology, phylogeny or evolution of C. botulinum. The recent determination of the genome sequence of C. botulinum has allowed comparative genomic indexing using a DNA microarray. Whole genome microarray analysis revealed that 63% of the coding sequences (CDSs) present in reference strain ATCC 3502 were common to all 61 widely-representative strains of proteolytic C. botulinum and the closely related C. sporogenes tested. This indicates a relatively stable genome. There was, however, evidence for recombination and genetic exchange, in particular within the neurotoxin gene and cluster (including transfer of neurotoxin genes to C. sporogenes), and the flagellar glycosylation island (FGI). These two loci appear to have evolved independently from each other, and from the remainder of the genetic complement. A number of strains were atypical; for example, while 10 out of 14 strains that formed type A1 toxin gave almost identical profiles in whole genome, neurotoxin cluster and FGI analyses, the other four strains showed divergent properties. Furthermore, a new neurotoxin sub-type (A5) has been discovered in strains from heroin-associated wound botulism cases. For the first time, differences in glycosylation profiles of the flagella could be linked to differences in the gene content of the FGI. Proteolytic C. botulinum has a stable genome backbone containing specific regions of genetic heterogeneity. These include the neurotoxin gene cluster and the FGI, each having evolved independently of each other and the remainder of the genetic complement. Analysis of these genetic components provides a high degree of discrimination of strains of proteolytic C. botulinum, and is suitable for clinical and forensic investigations of botulism outbreaks.

  15. Independent evolution of neurotoxin and flagellar genetic loci in proteolytic Clostridium botulinum

    Directory of Open Access Journals (Sweden)

    Twine Susan M

    2009-03-01

    Full Text Available Abstract Background Proteolytic Clostridium botulinum is the causative agent of botulism, a severe neuroparalytic illness. Given the severity of botulism, surprisingly little is known of the population structure, biology, phylogeny or evolution of C. botulinum. The recent determination of the genome sequence of C. botulinum has allowed comparative genomic indexing using a DNA microarray. Results Whole genome microarray analysis revealed that 63% of the coding sequences (CDSs present in reference strain ATCC 3502 were common to all 61 widely-representative strains of proteolytic C. botulinum and the closely related C. sporogenes tested. This indicates a relatively stable genome. There was, however, evidence for recombination and genetic exchange, in particular within the neurotoxin gene and cluster (including transfer of neurotoxin genes to C. sporogenes, and the flagellar glycosylation island (FGI. These two loci appear to have evolved independently from each other, and from the remainder of the genetic complement. A number of strains were atypical; for example, while 10 out of 14 strains that formed type A1 toxin gave almost identical profiles in whole genome, neurotoxin cluster and FGI analyses, the other four strains showed divergent properties. Furthermore, a new neurotoxin sub-type (A5 has been discovered in strains from heroin-associated wound botulism cases. For the first time, differences in glycosylation profiles of the flagella could be linked to differences in the gene content of the FGI. Conclusion Proteolytic C. botulinum has a stable genome backbone containing specific regions of genetic heterogeneity. These include the neurotoxin gene cluster and the FGI, each having evolved independently of each other and the remainder of the genetic complement. Analysis of these genetic components provides a high degree of discrimination of strains of proteolytic C. botulinum, and is suitable for clinical and forensic investigations of botulism

  16. The flagellar master operon flhDC is a pleiotropic regulator involved in motility and virulence of the fish pathogen Yersinia ruckeri

    Science.gov (United States)

    Aims: To investigate the function of the master flagellar operon flhDC in the fish pathogen Yersinia ruckeri and compare the effect of flhD mutation to a naturally occurring mutation causing loss-of-motility in emergent biotype 2 (BT2) strains. Methods and Results: In this study isogenic Y. ruckeri ...

  17. Ultrastructure of the harmful unarmored dinoflagellate Cochlodinium polykrikoides (Dinophyceae) with reference to the apical groove and flagellar apparatus

    DEFF Research Database (Denmark)

    Iwataki, Mitsunori; Hansen, Gert; Moestrup, Øjvind

    2010-01-01

    The external and internal ultrastructure of the harmful unarmored dinoflagellate Cochlodinium polykrikoides Margalef has been examined with special reference to the apical groove and three-dimensional structure of the flagellar apparatus. The apical groove is U-shaped and connected to the anterior...

  18. The electric motor handbook

    Energy Technology Data Exchange (ETDEWEB)

    Hurst, R.W.; Feltham, P. (eds.)

    2004-05-01

    This handbook outlines the important role that electric motors play in modern society. It covers the field of motor applications from various motor types to their use and repair. It also presents practical applications of electric motors and methods on motor efficiency. More than half of all electricity generated, and 75 per cent of all industrial electricity consumption is consumed by electric motors. Electrical personnel must be aware of all factors involved in electric motors in order to choose and apply the appropriate size of electric motor. These factors include efficiency, sizing and proper application. The efficient use and maximum life expectancy of electric motors depends on proper motor protection, control and maintenance. This handbook includes articles from leading experts on electric motors in modern electrical systems. The content includes: design considerations; proper electric motor sizing techniques; optimal electric motor application; electric motor protection technology; electric motor control principles; electric motor maintenance and troubleshooting; induction electric motors; electric motor bearing currents; electric motor bearing lubrication; electromagnetism; electric motor enclosures; electric motor testing; electric motor repair; DC electric motor; electric motor starters; electric motor brushes; industrial electric motors; electric motor diagrams; AC electric motors; electric motor wiring; electric motor service; electric motor rewinding; electric motor winding; diagram of electric motor wiring; electric motor kit; and, troubleshooting electric motors. A directory of motor manufacturers and suppliers was also included. refs., tabs., figs.

  19. Emergence of flagellar beating from the collective behavior of individual ATP-powered dyneins

    NARCIS (Netherlands)

    Namdeo, S.; Onck, P. R.

    2016-01-01

    Flagella are hair-like projections from the surface of eukaryotic cells, and they play an important role in many cellular functions, such as cell-motility. The beating of flagella is enabled by their internal architecture, the axoneme, and is powered by a dense distribution of motor proteins,

  20. Impaired competence in flagellar mutants of Bacillus subtilis is connected to the regulatory network governed by DegU

    DEFF Research Database (Denmark)

    Hölscher, Theresa; Schiklang, Tina; Dragos, Anna

    2018-01-01

    The competent state is a developmentally distinct phase, in which bacteria are able to take up and integrate exogenous DNA into their genome. Bacillus subtilis is one of the naturally competent bacterial species and the domesticated laboratory strain 168 is easily transformable. In this study, we...... report a reduced transformation frequency of B. subtilis mutants lacking functional and structural flagellar components. This includes hag, the gene encoding the flagellin protein forming the filament of the flagellum. We confirm that the observed decrease of the transformation frequency is due...... a close link between motility and natural competence in B. subtilis suggesting that hindrance in motility has great impact on differentiation of this bacterium not restricted only to the transition towards sessile growth stage....

  1. Interaction between the flagellar pocket collar and the hook complex via a novel microtubule-binding protein in Trypanosoma brucei.

    Directory of Open Access Journals (Sweden)

    Anna Albisetti

    2017-11-01

    Full Text Available Trypanosoma brucei belongs to a group of unicellular, flagellated parasites that are responsible for human African trypanosomiasis. An essential aspect of parasite pathogenicity is cytoskeleton remodelling, which occurs during the life cycle of the parasite and is accompanied by major changes in morphology and organelle positioning. The flagellum originates from the basal bodies and exits the cell body through the flagellar pocket (FP but remains attached to the cell body via the flagellum attachment zone (FAZ. The FP is an invagination of the pellicular membrane and is the sole site for endo- and exocytosis. The FAZ is a large complex of cytoskeletal proteins, plus an intracellular set of four specialised microtubules (MtQ that elongate from the basal bodies to the anterior end of the cell. At the distal end of the FP, an essential, intracellular, cytoskeletal structure called the flagellar pocket collar (FPC circumvents the flagellum. Overlapping the FPC is the hook complex (HC (a sub-structure of the previously named bilobe that is also essential and is thought to be involved in protein FP entry. BILBO1 is the only functionally characterised FPC protein and is necessary for FPC and FP biogenesis. Here, we used a combination of in vitro and in vivo approaches to identify and characterize a new BILBO1 partner protein-FPC4. We demonstrate that FPC4 localises to the FPC, the HC, and possibly to a proximal portion of the MtQ. We found that the C-terminal domain of FPC4 interacts with the BILBO1 N-terminal domain, and we identified the key amino acids required for this interaction. Interestingly, the FPC4 N-terminal domain was found to bind microtubules. Over-expression studies highlight the role of FPC4 in its association with the FPC, HC and FPC segregation. Our data suggest a tripartite association between the FPC, the HC and the MtQ.

  2. Cationic amino acid uptake constitutes a metabolic regulation mechanism and occurs in the flagellar pocket of Trypanosoma cruzi.

    Directory of Open Access Journals (Sweden)

    Mariana R Miranda

    Full Text Available Trypanosomatids' amino acid permeases are key proteins in parasite metabolism since they participate in the adaptation of parasites to different environments. Here, we report that TcAAP3, a member of a Trypanosoma cruzi multigene family of permeases, is a bona fide arginine transporter. Most higher eukaryotic cells incorporate cationic amino acids through a single transporter. In contrast, T. cruzi can recognize and transport cationic amino acids by mono-specific permeases since a 100-fold molar excess of lysine could not affect the arginine transport in parasites that over-express the arginine permease (TcAAP3 epimastigotes. In order to test if the permease activity regulates downstream processes of the arginine metabolism, the expression of the single T. cruzi enzyme that uses arginine as substrate, arginine kinase, was evaluated in TcAAP3 epimastigotes. In this parasite model, intracellular arginine concentration increases 4-folds and ATP level remains constant until cultures reach the stationary phase of growth, with decreases of about 6-folds in respect to the controls. Interestingly, Western Blot analysis demonstrated that arginine kinase is significantly down-regulated during the stationary phase of growth in TcAAP3 epimastigotes. This decrease could represent a compensatory mechanism for the increase in ATP consumption as a consequence of the displacement of the reaction equilibrium of arginine kinase, when the intracellular arginine concentration augments and the glucose from the medium is exhausted. Using immunofluorescence techniques we also determined that TcAAP3 and the specific lysine transporter TcAAP7 co-localize in a specialized region of the plasma membrane named flagellar pocket, staining a single locus close to the flagellar pocket collar. Taken together these data suggest that arginine transport is closely related to arginine metabolism and cell energy balance. The clinical relevance of studying trypanosomatids' permeases

  3. Two Types of Genetic Interaction Implicate the Whirligig Gene of Drosophila Melanogaster in Microtubule Organization in the Flagellar Axoneme

    Science.gov (United States)

    Green, L. L.; Wolf, N.; McDonald, K. L.; Fuller, M. T.

    1990-01-01

    The mutant nc4 allele of whirligig (3-54.4) of Drosophila melanogaster fails to complement mutations in an α-tubulin locus, α1t, mutations in a β-tubulin locus, B2t, or a mutation in the haywire locus. However, wrl fails to map to any of the known α- or β-tubulin genes. The extragenic failure to complement could indicate that the wrl product participates in structural interactions with microtubule proteins. The whirligig locus appears to be haploinsufficient for male fertility. Both a deficiency of wrl and possible loss of function alleles obtained by reverting the failure to complement between wrl(nc4) and B2t(n) are dominant male sterile in a genetic background wild type for tubulin. The dominant male sterility of the revertant alleles is suppressed if the flies are also heterozygous for B2t(n), for a deficiency of α1t, or for the hay(nc2) allele. These results suggest that it is not the absolute level of wrl gene product but its level relative to tubulin or microtubule function that is important for normal spermatogenesis. The phenotype of homozygous wrl mutants suggests that the whirligig product plays a role in postmeiotic spermatid differentiation, possibly in organizing the microtubules of the sperm flagellar axoneme. Flies homozygous for either wrl(nc4) or revertant alleles are viable and female fertile but male sterile. Premeiotic and meiotic stages of spermatogenesis appear normal. However, in post-meiotic stages, flagellar axonemes show loss of the accessory microtubule on the B-subfiber of outer doublet microtubules, outer triplet instead of outer doublet microtubules, and missing central pair microtubules. PMID:2127579

  4. Motor homopolar

    OpenAIRE

    Martín Muñoz, Agustín

    2007-01-01

    Mostramos la construcción de un modelo de motor homopolar, uno de los más antiguos tipos de motores eléctricos. Se caracterizan porque el campo magnético del imán mantiene siempre la misma polaridad (de ahí su nombre, del griego homos, igual), de modo que, cuando una corriente eléctrica atraviesa el campo magnético, aparece una fuerza que hace girar los elementos no fijados mecánicamente. En el sencillísimo motor homopolar colgado (Schlichting y Ucke 2004), el imán puede girar ...

  5. Application of stepping motor

    International Nuclear Information System (INIS)

    1980-10-01

    This book is divided into three parts, which is about practical using of stepping motor. The first part has six chapters. The contents of the first part are about stepping motor, classification of stepping motor, basic theory og stepping motor, characteristic and basic words, types and characteristic of stepping motor in hybrid type and basic control of stepping motor. The second part deals with application of stepping motor with hardware of stepping motor control, stepping motor control by microcomputer and software of stepping motor control. The last part mentions choice of stepping motor system, examples of stepping motor, measurement of stepping motor and practical cases of application of stepping motor.

  6. Curcumin Reduces the Motility of Salmonella enterica Serovar Typhimurium by Binding to the Flagella, Thereby Leading to Flagellar Fragility and Shedding

    Science.gov (United States)

    Balakrishnan, Arjun; Negi, Vidya Devi; Sakorey, Deepika; Chandra, Nagasuma

    2016-01-01

    ABSTRACT One of the important virulence properties of the pathogen is its ability to travel to a favorable environment, cross the viscous mucus barrier (intestinal barrier for enteric pathogens), and reach the epithelia to initiate pathogenesis with the help of an appendage, like flagella. Nonetheless, flagella can act as an “Achilles heel,” revealing the pathogen's presence to the host through the stimulation of innate and adaptive immune responses. We assessed whether curcumin, a dietary polyphenol, could alter the motility of Salmonella, a foodborne pathogen. It reduced the motility of Salmonella enterica serovar Typhimurium by shortening the length of the flagellar filament (from ∼8 μm to ∼5 μm) and decreasing its density (4 or 5 flagella/bacterium instead of 8 or 9 flagella/bacterium). Upon curcumin treatment, the percentage of flagellated bacteria declined from ∼84% to 59%. However, no change was detected in the expression of the flagellin gene and protein. A fluorescence binding assay demonstrated binding of curcumin to the flagellar filament. This might make the filament fragile, breaking it into smaller fragments. Computational analysis predicted the binding of curcumin, its analogues, and its degraded products to a flagellin molecule at an interface between domains D1 and D2. Site-directed mutagenesis and a fluorescence binding assay confirmed the binding of curcumin to flagellin at residues ASN120, ASP123, ASN163, SER164, ASN173, and GLN175. IMPORTANCE This work, to our knowledge the first report of its kind, examines how curcumin targets flagellar density and affects the pathogenesis of bacteria. We found that curcumin does not affect any of the flagellar synthesis genes. Instead, it binds to the flagellum and makes it fragile. It increases the torsional stress on the flagellar filament that then breaks, leaving fewer flagella around the bacteria. Flagella, which are crucial ligands for Toll-like receptor 5, are some of the most important

  7. The Sensor Kinase GacS Negatively Regulates Flagellar Formation and Motility in a Biocontrol Bacterium, Pseudomonas chlororaphis O6

    Directory of Open Access Journals (Sweden)

    Ji Soo Kim

    2014-06-01

    Full Text Available The GacS/GacA two component system regulates various traits related to the biocontrol potential of plant-associated pseudomonads. The role of the sensor kinase, GacS, differs between strains in regulation of motility. In this study, we determined how a gacS mutation changed cell morphology and motility in Pseudomonas chlororaphis O6. The gacS mutant cells were elongated in stationary-phase compared to the wild type and the complemented gacS mutant, but cells did not differ in length in logarithmic phase. The gacS mutant had a two-fold increase in the number of flagella compared with the wild type strain; flagella number was restored to that of the wild type in the complemented gacS mutant. The more highly flagellated gacS mutant cells had greater swimming motilities than that of the wild type strain. Enhanced flagella formation in the gacS mutant correlated with increased expression of three genes, fleQ, fliQ and flhF, involved in flagellar formation. Expression of these genes in the complemented gacS mutant was similar to that of the wild type. These findings show that this root-colonizing pseudomonad adjusts flagella formation and cell morphology in stationary-phase using GacS as a major regulator.

  8. A novel flagellar sheath protein, FcpA, determines filament coiling, translational motility and virulence for the Leptospira spirochete.

    Science.gov (United States)

    Wunder, Elsio A; Figueira, Cláudio P; Benaroudj, Nadia; Hu, Bo; Tong, Brian A; Trajtenberg, Felipe; Liu, Jun; Reis, Mitermayer G; Charon, Nyles W; Buschiazzo, Alejandro; Picardeau, Mathieu; Ko, Albert I

    2016-08-01

    Leptospira are unique among bacteria based on their helical cell morphology with hook-shaped ends and the presence of periplasmic flagella (PF) with pronounced spontaneous supercoiling. The factors that provoke such supercoiling, as well as the role that PF coiling plays in generating the characteristic hook-end cell morphology and motility, have not been elucidated. We have now identified an abundant protein from the pathogen L. interrogans, exposed on the PF surface, and named it Flagellar-coiling protein A (FcpA). The gene encoding FcpA is highly conserved among Leptospira and was not found in other bacteria. fcpA(-) mutants, obtained from clinical isolates or by allelic exchange, had relatively straight, smaller-diameter PF, and were not able to produce translational motility. These mutants lost their ability to cause disease in the standard hamster model of leptospirosis. Complementation of fcpA restored the wild-type morphology, motility and virulence phenotypes. In summary, we identified a novel Leptospira 36-kDa protein, the main component of the spirochete's PF sheath, and a key determinant of the flagella's coiled structure. FcpA is essential for bacterial translational motility and to enable the spirochete to penetrate the host, traverse tissue barriers, disseminate to cause systemic infection and reach target organs. © 2016 John Wiley & Sons Ltd.

  9. Zernike phase contrast cryo-electron tomography of sodium-driven flagellar hook-basal bodies from Vibrio alginolyticus.

    Science.gov (United States)

    Hosogi, Naoki; Shigematsu, Hideki; Terashima, Hiroyuki; Homma, Michio; Nagayama, Kuniaki

    2011-01-01

    Vibrio alginolyticus use flagella to swim. A flagellum consists of a filament, hook and basal body. The basal body is made up of a rod and several ring structures. This study investigates the structure of the T ring which is a unique component of the V. alginolyticus sodium ion-driven flagellar basal body. Using Zernike phase contrast (ZPC) cryo-electron tomography, we compared the 3D structures of purified hook-basal bodies (HBB) from a wild-type strain (KK148) and a deletion mutant lacking MotX and MotY (TH3), which are thought to form the T ring. ZPC images of HBBs had highly improved signal-to-noise ratio compared to conventional phase contrast images. We observed the outline of the HBBs from strains KK148 and TH3, and the TH3 mutant was missing its T ring. In the wild-type strain, the T ring was beneath the LP ring and seemed to form a ring shape with diameter of 32 nm. Copyright © 2010 Elsevier Inc. All rights reserved.

  10. Jidosha's Motors

    OpenAIRE

    Shirakawa Okuma, Rosely; Calderón Orejuela, Javier

    2016-01-01

    La tesis narra la situación de una empresa concesionaria de vehículos nuevos, Jidosha's Motors, perteneciente a una corporación japonesa que cuenta con una cultura muy arraigada de ética y de cumplimiento. Se plantean respuestas, se identifican problemas y sus alternativas de solución para una toma adecuada de decisiones por parte de los directivos, siguiendo una estructura de análisis de situaciones de negocios (ASN). Tesis

  11. Complex Interplay between FleQ, Cyclic Diguanylate and Multiple σ Factors Coordinately Regulates Flagellar Motility and Biofilm Development in Pseudomonas putida.

    Directory of Open Access Journals (Sweden)

    Alicia Jiménez-Fernández

    Full Text Available Most bacteria alternate between a free living planktonic lifestyle and the formation of structured surface-associated communities named biofilms. The transition between these two lifestyles requires a precise and timely regulation of the factors involved in each of the stages that has been likened to a developmental process. Here we characterize the involvement of the transcriptional regulator FleQ and the second messenger cyclic diguanylate in the coordinate regulation of multiple functions related to motility and surface colonization in Pseudomonas putida. Disruption of fleQ caused strong defects in flagellar motility, biofilm formation and surface attachment, and the ability of this mutation to suppress multiple biofilm-related phenotypes associated to cyclic diguanylate overproduction suggests that FleQ mediates cyclic diguanylate signaling critical to biofilm growth. We have constructed a library containing 94 promoters potentially involved in motility and biofilm development fused to gfp and lacZ, screened this library for FleQ and cyclic diguanylate regulation, and assessed the involvement of alternative σ factors σN and FliA in the transcription of FleQ-regulated promoters. Our results suggest a dual mode of action for FleQ. Low cyclic diguanylate levels favor FleQ interaction with σN-dependent promoters to activate the flagellar cascade, encompassing the flagellar cluster and additional genes involved in cyclic diguanylate metabolism, signal transduction and gene regulation. On the other hand, characterization of the FleQ-regulated σN- and FliA-independent PlapA and PbcsD promoters revealed two disparate regulatory mechanisms leading to a similar outcome: the synthesis of biofilm matrix components in response to increased cyclic diguanylate levels.

  12. Proteomic Analysis of Intact Flagella of Procyclic Trypanosoma brucei Cells Identifies Novel Flagellar Proteins with Unique Sub-localization and Dynamics*

    Science.gov (United States)

    Subota, Ines; Julkowska, Daria; Vincensini, Laetitia; Reeg, Nele; Buisson, Johanna; Blisnick, Thierry; Huet, Diego; Perrot, Sylvie; Santi-Rocca, Julien; Duchateau, Magalie; Hourdel, Véronique; Rousselle, Jean-Claude; Cayet, Nadège; Namane, Abdelkader; Chamot-Rooke, Julia; Bastin, Philippe

    2014-01-01

    Cilia and flagella are complex organelles made of hundreds of proteins of highly variable structures and functions. Here we report the purification of intact flagella from the procyclic stage of Trypanosoma brucei using mechanical shearing. Structural preservation was confirmed by transmission electron microscopy that showed that flagella still contained typical elements such as the membrane, the axoneme, the paraflagellar rod, and the intraflagellar transport particles. It also revealed that flagella severed below the basal body, and were not contaminated by other cytoskeletal structures such as the flagellar pocket collar or the adhesion zone filament. Mass spectrometry analysis identified a total of 751 proteins with high confidence, including 88% of known flagellar components. Comparison with the cell debris fraction revealed that more than half of the flagellum markers were enriched in flagella and this enrichment criterion was taken into account to identify 212 proteins not previously reported to be associated to flagella. Nine of these were experimentally validated including a 14-3-3 protein not yet reported to be associated to flagella and eight novel proteins termed FLAM (FLAgellar Member). Remarkably, they localized to five different subdomains of the flagellum. For example, FLAM6 is restricted to the proximal half of the axoneme, no matter its length. In contrast, FLAM8 is progressively accumulating at the distal tip of growing flagella and half of it still needs to be added after cell division. A combination of RNA interference and Fluorescence Recovery After Photobleaching approaches demonstrated very different dynamics from one protein to the other, but also according to the stage of construction and the age of the flagellum. Structural proteins are added to the distal tip of the elongating flagellum and exhibit slow turnover whereas membrane proteins such as the arginine kinase show rapid turnover without a detectible polarity. PMID:24741115

  13. Proteomic analysis of intact flagella of procyclic Trypanosoma brucei cells identifies novel flagellar proteins with unique sub-localization and dynamics.

    Science.gov (United States)

    Subota, Ines; Julkowska, Daria; Vincensini, Laetitia; Reeg, Nele; Buisson, Johanna; Blisnick, Thierry; Huet, Diego; Perrot, Sylvie; Santi-Rocca, Julien; Duchateau, Magalie; Hourdel, Véronique; Rousselle, Jean-Claude; Cayet, Nadège; Namane, Abdelkader; Chamot-Rooke, Julia; Bastin, Philippe

    2014-07-01

    Cilia and flagella are complex organelles made of hundreds of proteins of highly variable structures and functions. Here we report the purification of intact flagella from the procyclic stage of Trypanosoma brucei using mechanical shearing. Structural preservation was confirmed by transmission electron microscopy that showed that flagella still contained typical elements such as the membrane, the axoneme, the paraflagellar rod, and the intraflagellar transport particles. It also revealed that flagella severed below the basal body, and were not contaminated by other cytoskeletal structures such as the flagellar pocket collar or the adhesion zone filament. Mass spectrometry analysis identified a total of 751 proteins with high confidence, including 88% of known flagellar components. Comparison with the cell debris fraction revealed that more than half of the flagellum markers were enriched in flagella and this enrichment criterion was taken into account to identify 212 proteins not previously reported to be associated to flagella. Nine of these were experimentally validated including a 14-3-3 protein not yet reported to be associated to flagella and eight novel proteins termed FLAM (FLAgellar Member). Remarkably, they localized to five different subdomains of the flagellum. For example, FLAM6 is restricted to the proximal half of the axoneme, no matter its length. In contrast, FLAM8 is progressively accumulating at the distal tip of growing flagella and half of it still needs to be added after cell division. A combination of RNA interference and Fluorescence Recovery After Photobleaching approaches demonstrated very different dynamics from one protein to the other, but also according to the stage of construction and the age of the flagellum. Structural proteins are added to the distal tip of the elongating flagellum and exhibit slow turnover whereas membrane proteins such as the arginine kinase show rapid turnover without a detectible polarity. © 2014 by The

  14. The flagellar master operon flhDC is a pleiotropic regulator involved in motility and virulence of the fish pathogen Yersinia ruckeri.

    Science.gov (United States)

    Jozwick, A K S; Graf, J; Welch, T J

    2017-03-01

    To investigate the function of the master flagellar operon flhDC in the fish pathogen Yersinia ruckeri and compare the effect of a constructed flhD mutation to a naturally occurring fliR mutation causing loss-of-motility in emergent biotype 2 (BT2) strains. Yersinia ruckeri flhD and fliR mutants were constructed in a motile strain. Both mutations caused loss-of-motility, ablation of flagellin synthesis and phospholipase secretion, similar to naturally occurring BT2 strains. Transcriptome analysis confirmed flhDC regulation of flagellar, chemotaxis and phospholipase loci as well as other genes of diverse function. The flhD mutation confers a competitive advantage within the fish host when compared with its parent strain, while this advantage was not seen with the naturally occurring fliR mutation. An intact flhD is necessary for expression of the flagellar secretion system as well as other diverse loci, consistent with a role for flhD as a pleiotropic regulator. The maintenance of the flhD locus in Y. ruckeri strains suggests its importance for aspects of Y. ruckeri biology other than virulence, since the flhD mutation conferred a competitive advantage during experimental challenge of rainbow trout. Yersinia ruckeri is the causative agent of enteric red mouth disease, an invasive septicaemia that affects farmed salmonid fish species. Disease outbreaks can cause severe economic losses in aquaculture. BT2 variants, which have independently emerged worldwide, are an increasing threat to farmed fish production. Knowledge of mechanisms involved in virulence, conserved functions and gene regulation among strains may be exploited for the development of novel disease control strategies to prevent pathogen growth or virulence phenotypes within aquaculture. Published 2016. This article is a U.S. Government work and is in the public domain in the USA.

  15. ATG24 Represses Autophagy and Differentiation and Is Essential for Homeostasy of the Flagellar Pocket in Trypanosoma brucei.

    Directory of Open Access Journals (Sweden)

    Ana Brennand

    Full Text Available We have previously identified homologs for nearly half of the approximately 30 known yeast Atg's in the genome database of the human sleeping sickness parasite Trypanosoma brucei. So far, only a few of these homologs have their role in autophagy experimentally confirmed. Among the candidates was the ortholog of Atg24 that is involved in pexophagy in yeast. In T. brucei, the peroxisome-like organelles named glycosomes harbor core metabolic processes, especially glycolysis. In the autotrophic yeast, autophagy is essential for adaptation to different nutritional environments by participating in the renewal of the peroxisome population. We hypothesized that autophagic turnover of the parasite's glycosomes plays a role in differentiation during its life cycle, which demands adaptation to different host environments and associated dramatic changes in nutritional conditions. We therefore characterized T. brucei ATG24, the T. brucei ortholog of yeast Atg24 and mammalian SNX4, and found it to have a regulatory role in autophagy and differentiation as well as endocytic trafficking. ATG24 partially localized on endocytic membranes where it was recruited via PI3-kinase III/VPS34. ATG24 silencing severely impaired receptor-mediated endocytosis of transferrin, but not adsorptive uptake of a lectin, and caused a major enlargement of the flagellar pocket. ATG24 silencing approximately doubled the number of autophagosomes, suggesting a role in repressing autophagy, and strongly accelerated differentiation, in accordance with a role of autophagy in parasite differentiation. Overexpression of the two isoforms of T. brucei ATG8 fused to GFP slowed down differentiation, possibly by a dominant-negative effect. This was overcome by ATG24 depletion, further supporting its regulatory role.

  16. Two new species of Piaroa (Arachnida: Schizomida, Hubbardiidae) from Colombia, with comments on the genus taxonomy and the flagellar setae pattern of Hubbardiinae.

    Science.gov (United States)

    Moreno-González, Jairo A; Delgado-Santa, Leonardo; De Armas, Luis F

    2014-08-14

    Two new species of the genus Piaroa Villarreal, Tourinho & Giupponi, 2008, P. escalerete sp. nov. and P. bacata sp. nov. are described from Valle del Cauca, and Cundinamarca departments, Colombia, respectively. The female flagellum is fully illustrated for a Piaroa species for the first time; the generic diagnosis is also emended and the relationships of the new species with those previously described are discussed. New characters for Piaroa species, a new nomenclature for the chitinized arch and a reinterpretation of the Hubbardiinae flagellar setae pattern are proposed. A distribution map of the known species of Piaroa is provided. 

  17. Fine motor control

    Science.gov (United States)

    ... gross (large, general) motor control. An example of gross motor control is waving an arm in greeting. Problems ... out the child's developmental age. Children develop fine motor skills over time, by practicing and being taught. To ...

  18. A Xanthomonas citri subsp citri hypothetical protein related to virulence contains a non-functional HD domain and is implicated in flagellar motility.

    Science.gov (United States)

    Vieira, F C F; Gonçalves, A M; Mendoza, E F R; Ferreira, R M; Costa, M L M; Balbuena, T S; Sebinelli, H G; Ciancaglini, P; Pizauro Junior, J M; Ferro, J A

    2017-08-31

    Citrus canker, caused by the Gram-negative bacterium Xanthomonas citri subsp citri (Xac), severely affects most economically important citrus varieties worldwide. A previous study showed that disruption of the ORF XAC1201 from the Xac 306 strain by transposon Tn5 decreased bacterium virulence in the Rangpur lime host (Citrus limonia L. Osbeck). However, little is known regarding the possible function of the hypothetical protein XAC1201 and how it affects the virulence of Xac 306. Here, we confirmed that disruption of ORF XAC1201 reduces Xac 306 virulence in two different hosts, delaying the onset of typical symptoms. In silico analysis suggested that XAC1201 interacts with the flagellar proteins FliM and FliL, known to be an important factor for virulence. In fact, motility assays revealed that the XAC1201 mutant has a significant difference in motility compared to the wild-type Xac 306. Also, a 3-D structure model revealed modified cofactor binding sites and suggested that XAC1201 has a non-functional HD domain. This hypothesis was confirmed by enzymatic assays performed in purified, XAC1201 recombinant protein expressed in Escherichia coli, which revealed no significant activities previously associated with HD domains for the tested substrates. Thus, the role of the XAC1201 protein in Xac 306 virulence seems to be related to flagellar motility, although a non-classic role for the HD domain cannot be dismissed.

  19. Cloning, purification, crystallization and preliminary X-ray studies of flagellar hook scaffolding protein FlgD from Pseudomonas aeruginosa PAO1

    International Nuclear Information System (INIS)

    Luo, Miao; Niu, Siqiang; Yin, Yibing; Huang, Ailong; Wang, Deqiang

    2009-01-01

    In order to better elucidate the functions of FlgD in flagellar hook biosynthesis, the three-dimensional structure of FlgD is being determined by X-ray crystallography. Here, the expression, purification, crystallization and preliminary crystallographic analysis of FlgD from P. aeruginosa are reported. FlgD regulates the assembly of the hook cap structure to prevent leakage of hook monomers into the medium and hook monomer polymerization and also plays a role in determination of the correct hook length, with the help of the FliK protein. In order to better elucidate the functions of FlgD in flagellar hook biosynthesis, the three-dimensional structure of FlgD is being determined by X-ray crystallography. Here, the expression, purification, crystallization and preliminary crystallographic analysis of FlgD from P. aeruginosa are reported. The crystal belonged to space group I222 and diffracted to a resolution of 2.5 Å, with unit-cell parameters a = 116.47, b = 118.71, c = 118.85 Å. The crystals are most likely to contain three molecules in the asymmetric unit, with a V M value of 2.73 Å 3 Da −1

  20. Motor control for a brushless DC motor

    Science.gov (United States)

    Peterson, William J. (Inventor); Faulkner, Dennis T. (Inventor)

    1985-01-01

    This invention relates to a motor control system for a brushless DC motor having an inverter responsively coupled to the motor control system and in power transmitting relationship to the motor. The motor control system includes a motor rotor speed detecting unit that provides a pulsed waveform signal proportional to rotor speed. This pulsed waveform signal is delivered to the inverter to thereby cause an inverter fundamental current waveform output to the motor to be switched at a rate proportional to said rotor speed. In addition, the fundamental current waveform is also pulse width modulated at a rate proportional to the rotor speed. A fundamental current waveform phase advance circuit is controllingly coupled to the inverter. The phase advance circuit is coupled to receive the pulsed waveform signal from the motor rotor speed detecting unit and phase advance the pulsed waveform signal as a predetermined function of motor speed to thereby cause the fundamental current waveform to be advanced and thereby compensate for fundamental current waveform lag due to motor winding reactance which allows the motor to operate at higher speeds than the motor is rated while providing optimal torque and therefore increased efficiency.

  1. A Proton-Driven Plasma Wakefield Acceleration experiment at CERN

    CERN Multimedia

    The AWAKE Collaboration has been formed in order to demonstrate protondriven plasma wakefield acceleration for the first time. This technology could lead to future colliders of high energy but of a much reduced length compared to proposed linear accelerators. The SPS proton beam in the CNGS facility will be injected into a 10m plasma cell where the long proton bunches will be modulated into significantly shorter micro-bunches. These micro-bunches will then initiate a strong wakefield in the plasma with peak fields above 1 GV/m that will be harnessed to accelerate a bunch of electrons from about 20MeV to the GeV scale within a few meters. The experimental program is based on detailed numerical simulations of beam and plasma interactions. The main accelerator components, the experimental area and infrastructure required as well as the plasma cell and the diagnostic equipment are discussed in detail. First protons to the experiment are expected at the end of 2016 and this will be followed by an initial 3–4 yea...

  2. High efficiency motors; Motores de alta eficiencia

    Energy Technology Data Exchange (ETDEWEB)

    Uranga Favela, Ivan Jaime [Energia Controlada de Mexico, S. A. de C. V., Mexico, D. F. (Mexico)

    1993-12-31

    This paper is a technical-financial study of the high efficiency and super-premium motors. As it is widely known, more than 60% of the electrical energy generated in the country is used for the operation of motors, in industry as well as in commerce. Therefore the importance that the motors have in the efficient energy use. [Espanol] El presente trabajo es un estudio tecnico-financiero de los motores de alta eficiencia y los motores super premium. Como es ampliamente conocido, mas del 60% de la energia electrica generada en el pais, es utilizada para accionar motores, dentro de la industria y el comercio. De alli la importancia que los motores tienen en el uso eficiente de la energia.

  3. High efficiency motors; Motores de alta eficiencia

    Energy Technology Data Exchange (ETDEWEB)

    Uranga Favela, Ivan Jaime [Energia Controlada de Mexico, S. A. de C. V., Mexico, D. F. (Mexico)

    1992-12-31

    This paper is a technical-financial study of the high efficiency and super-premium motors. As it is widely known, more than 60% of the electrical energy generated in the country is used for the operation of motors, in industry as well as in commerce. Therefore the importance that the motors have in the efficient energy use. [Espanol] El presente trabajo es un estudio tecnico-financiero de los motores de alta eficiencia y los motores super premium. Como es ampliamente conocido, mas del 60% de la energia electrica generada en el pais, es utilizada para accionar motores, dentro de la industria y el comercio. De alli la importancia que los motores tienen en el uso eficiente de la energia.

  4. Flagellar membrane fusion and protein exchange in trypanosomes; a new form of cell-cell communication? [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Simon Imhof

    2016-04-01

    Full Text Available Diverse structures facilitate direct exchange of proteins between cells, including plasmadesmata in plants and tunnelling nanotubes in bacteria and higher eukaryotes.  Here we describe a new mechanism of protein transfer, flagellar membrane fusion, in the unicellular parasite Trypanosoma brucei. When fluorescently tagged trypanosomes were co-cultured, a small proportion of double-positive cells were observed. The formation of double-positive cells was dependent on the presence of extracellular calcium and was enhanced by placing cells in medium supplemented with fresh bovine serum. Time-lapse microscopy revealed that double-positive cells arose by bidirectional protein exchange in the absence of nuclear transfer.  Furthermore, super-resolution microscopy showed that this process occurred in ≤1 minute, the limit of temporal resolution in these experiments. Both cytoplasmic and membrane proteins could be transferred provided they gained access to the flagellum. Intriguingly, a component of the RNAi machinery (Argonaute was able to move between cells, raising the possibility that small interfering RNAs are transported as cargo. Transmission electron microscopy showed that shared flagella contained two axonemes and two paraflagellar rods bounded by a single membrane. In some cases flagellar fusion was partial and interactions between cells were transient. In other cases fusion occurred along the entire length of the flagellum, was stable for several hours and might be irreversible. Fusion did not appear to be deleterious for cell function: paired cells were motile and could give rise to progeny while fused. The motile flagella of unicellular organisms are related to the sensory cilia of higher eukaryotes, raising the possibility that protein transfer between cells via cilia or flagella occurs more widely in nature.

  5. Electric motor handbook

    CERN Document Server

    Chalmers, B J

    2013-01-01

    Electric Motor Handbook aims to give practical knowledge in a wide range of capacities such as plant design, equipment specification, commissioning, operation and maintenance. The book covers topics such as the modeling of steady-state motor performance; polyphase induction, synchronous, and a.c. commutator motors; ambient conditions, enclosures, cooling and loss dissipation; and electrical supply systems and motor drives. Also covered are topics such as variable-speed drives and motor control; materials and motor components; insulation types, systems, and techniques; and the installation, sit

  6. Handbook on linear motor application

    International Nuclear Information System (INIS)

    1988-10-01

    This book guides the application for Linear motor. It lists classification and speciality of Linear Motor, terms of linear-induction motor, principle of the Motor, types on one-side linear-induction motor, bilateral linear-induction motor, linear-DC Motor on basic of the motor, linear-DC Motor for moving-coil type, linear-DC motor for permanent-magnet moving type, linear-DC motor for electricity non-utility type, linear-pulse motor for variable motor, linear-pulse motor for permanent magneto type, linear-vibration actuator, linear-vibration actuator for moving-coil type, linear synchronous motor, linear electromagnetic motor, linear electromagnetic solenoid, technical organization and magnetic levitation and linear motor and sensor.

  7. Interacting adiabatic quantum motor

    Science.gov (United States)

    Bruch, Anton; Kusminskiy, Silvia Viola; Refael, Gil; von Oppen, Felix

    2018-05-01

    We present a field-theoretic treatment of an adiabatic quantum motor. We explicitly discuss a motor called the Thouless motor which is based on a Thouless pump operating in reverse. When a sliding periodic potential is considered to be the motor degree of freedom, a bias voltage applied to the electron channel sets the motor in motion. We investigate a Thouless motor whose electron channel is modeled as a Luttinger liquid. Interactions increase the gap opened by the periodic potential. For an infinite Luttinger liquid the coupling-induced friction is enhanced by electron-electron interactions. When the Luttinger liquid is ultimately coupled to Fermi liquid reservoirs, the dissipation reduces to its value for a noninteracting electron system for a constant motor velocity. Our results can also be applied to a motor based on a nanomagnet coupled to a quantum spin Hall edge.

  8. Chronic motor tic disorder

    Science.gov (United States)

    Chronic vocal tic disorder; Tic - chronic motor tic disorder ... Chronic motor tic disorder is more common than Tourette syndrome . Chronic tics may be forms of Tourette syndrome. Tics usually start ...

  9. Piezoelectric Motors, an Overview

    OpenAIRE

    Karl Spanner; Burhanettin Koc

    2016-01-01

    Piezoelectric motors are used in many industrial and commercial applications. Various piezoelectric motors are available in the market. All of the piezoelectric motors use the inverse piezoelectric effect, where microscopically small oscillatory motions are converted into continuous or stepping rotary or linear motions. Methods of obtaining long moving distance have various drive and functional principles that make these motors categorized into three groups: resonance-drive (piezoelectric ult...

  10. Electric Motor Thermal Management

    Energy Technology Data Exchange (ETDEWEB)

    Bennion, Kevin S [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2017-09-01

    Thermal management enables more efficient and cost-effective motors. This Annual Merit Review presentation describes the technical accomplishments and progress in electric motor thermal management R&D over the last year. This project supports a broad industry demand for data, analysis methods, and experimental techniques to improve and better understand motor thermal management.

  11. Programmable dc motor controller

    Science.gov (United States)

    Hopwood, J. E.

    1982-11-01

    A portable programmable dc motor controller, with features not available on commercial instruments was developed for controlling fixtures during welding processes. The controller can be used to drive any dc motor having tachometer feedback and motor requirements not exceeding 30 volts, 3 amperes. Among the controller's features are delayed start time, upslope time, speed, and downslope time.

  12. Motor/generator

    Science.gov (United States)

    Hickam, Christopher Dale [Glasford, IL

    2008-05-13

    A motor/generator is provided for connecting between a transmission input shaft and an output shaft of a prime mover. The motor/generator may include a motor/generator housing, a stator mounted to the motor/generator housing, a rotor mounted at least partially within the motor/generator housing and rotatable about a rotor rotation axis, and a transmission-shaft coupler drivingly coupled to the rotor. The transmission-shaft coupler may include a clamp, which may include a base attached to the rotor and a plurality of adjustable jaws.

  13. Piezoelectric Motors, an Overview

    Directory of Open Access Journals (Sweden)

    Karl Spanner

    2016-02-01

    Full Text Available Piezoelectric motors are used in many industrial and commercial applications. Various piezoelectric motors are available in the market. All of the piezoelectric motors use the inverse piezoelectric effect, where microscopically small oscillatory motions are converted into continuous or stepping rotary or linear motions. Methods of obtaining long moving distance have various drive and functional principles that make these motors categorized into three groups: resonance-drive (piezoelectric ultrasonic motors, inertia-drive, and piezo-walk-drive. In this review, a comprehensive summary of piezoelectric motors, with their classification from initial idea to recent progress, is presented. This review also includes some of the industrial and commercial applications of piezoelectric motors that are presently available in the market as actuators.

  14. Motor degradation prediction methods

    Energy Technology Data Exchange (ETDEWEB)

    Arnold, J.R.; Kelly, J.F.; Delzingaro, M.J.

    1996-12-01

    Motor Operated Valve (MOV) squirrel cage AC motor rotors are susceptible to degradation under certain conditions. Premature failure can result due to high humidity/temperature environments, high running load conditions, extended periods at locked rotor conditions (i.e. > 15 seconds) or exceeding the motor`s duty cycle by frequent starts or multiple valve stroking. Exposure to high heat and moisture due to packing leaks, pressure seal ring leakage or other causes can significantly accelerate the degradation. ComEd and Liberty Technologies have worked together to provide and validate a non-intrusive method using motor power diagnostics to evaluate MOV rotor condition and predict failure. These techniques have provided a quick, low radiation dose method to evaluate inaccessible motors, identify degradation and allow scheduled replacement of motors prior to catastrophic failures.

  15. Neuroplasticity & Motor Learning

    DEFF Research Database (Denmark)

    Jensen, Jesper Lundbye

    Practice of a new motor task is usually associated with an improvement in performance. Indeed, if we stop practicing and return the next day to the same task, we find that our performance has been maintained and may even be better than it was at the start of the first day. This improvement...... is a measure of our ability to form and store a motor memory of the task. However, the initial memory of the task is labile and may be subject to interference. During and following motor learning plastic changes occur within the central nervous system. On one hand these changes are driven by motor practice......, on the other hand the changes underlie the formation of motor memory and the retention of improved motor performance. During motor learning changes may occur at many different levels within the central nervous system dependent on the type of task and training. Here, we demonstrate different studies from our...

  16. Unique ATPase site architecture triggers cis-mediated synchronized ATP binding in heptameric AAA+-ATPase domain of flagellar regulatory protein FlrC.

    Science.gov (United States)

    Dey, Sanjay; Biswas, Maitree; Sen, Udayaditya; Dasgupta, Jhimli

    2015-04-03

    Bacterial enhancer-binding proteins (bEBPs) oligomerize through AAA(+) domains and use ATP hydrolysis-driven energy to isomerize the RNA polymerase-σ(54) complex during transcriptional initiation. Here, we describe the first structure of the central AAA(+) domain of the flagellar regulatory protein FlrC (FlrC(C)), a bEBP that controls flagellar synthesis in Vibrio cholerae. Our results showed that FlrC(C) forms heptamer both in nucleotide (Nt)-free and -bound states without ATP-dependent subunit remodeling. Unlike the bEBPs such as NtrC1 or PspF, a novel cis-mediated "all or none" ATP binding occurs in the heptameric FlrC(C), because constriction at the ATPase site, caused by loop L3 and helix α7, restricts the proximity of the trans-protomer required for Nt binding. A unique "closed to open" movement of Walker A, assisted by trans-acting "Glu switch" Glu-286, facilitates ATP binding and hydrolysis. Fluorescence quenching and ATPase assays on FlrC(C) and mutants revealed that although Arg-349 of sensor II, positioned by trans-acting Glu-286 and Tyr-290, acts as a key residue to bind and hydrolyze ATP, Arg-319 of α7 anchors ribose and controls the rate of ATP hydrolysis by retarding the expulsion of ADP. Heptameric state of FlrC(C) is restored in solution even with the transition state mimicking ADP·AlF3. Structural results and pulldown assays indicated that L3 renders an in-built geometry to L1 and L2 causing σ(54)-FlrC(C) interaction independent of Nt binding. Collectively, our results underscore a novel mechanism of ATP binding and σ(54) interaction that strives to understand the transcriptional mechanism of the bEBPs, which probably interact directly with the RNA polymerase-σ(54) complex without DNA looping. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

  17. Motor degradation prediction methods

    International Nuclear Information System (INIS)

    Arnold, J.R.; Kelly, J.F.; Delzingaro, M.J.

    1996-01-01

    Motor Operated Valve (MOV) squirrel cage AC motor rotors are susceptible to degradation under certain conditions. Premature failure can result due to high humidity/temperature environments, high running load conditions, extended periods at locked rotor conditions (i.e. > 15 seconds) or exceeding the motor's duty cycle by frequent starts or multiple valve stroking. Exposure to high heat and moisture due to packing leaks, pressure seal ring leakage or other causes can significantly accelerate the degradation. ComEd and Liberty Technologies have worked together to provide and validate a non-intrusive method using motor power diagnostics to evaluate MOV rotor condition and predict failure. These techniques have provided a quick, low radiation dose method to evaluate inaccessible motors, identify degradation and allow scheduled replacement of motors prior to catastrophic failures

  18. Cryogenic Electric Motor Tested

    Science.gov (United States)

    Brown, Gerald V.

    2004-01-01

    Technology for pollution-free "electric flight" is being evaluated in a number of NASA Glenn Research Center programs. One approach is to drive propulsive fans or propellers with electric motors powered by fuel cells running on hydrogen. For large transport aircraft, conventional electric motors are far too heavy to be feasible. However, since hydrogen fuel would almost surely be carried as liquid, a propulsive electric motor could be cooled to near liquid hydrogen temperature (-423 F) by using the fuel for cooling before it goes to the fuel cells. Motor windings could be either superconducting or high purity normal copper or aluminum. The electrical resistance of pure metals can drop to 1/100th or less of their room-temperature resistance at liquid hydrogen temperature. In either case, super or normal, much higher current density is possible in motor windings. This leads to more compact motors that are projected to produce 20 hp/lb or more in large sizes, in comparison to on the order of 2 hp/lb for large conventional motors. High power density is the major goal. To support cryogenic motor development, we have designed and built in-house a small motor (7-in. outside diameter) for operation in liquid nitrogen.

  19. Hybrid vehicle motor alignment

    Science.gov (United States)

    Levin, Michael Benjamin

    2001-07-03

    A rotor of an electric motor for a motor vehicle is aligned to an axis of rotation for a crankshaft of an internal combustion engine having an internal combustion engine and an electric motor. A locator is provided on the crankshaft, a piloting tool is located radially by the first locator to the crankshaft. A stator of the electric motor is aligned to a second locator provided on the piloting tool. The stator is secured to the engine block. The rotor is aligned to the crankshaft and secured thereto.

  20. Crystallization and preliminary X-ray analysis of a C-terminal fragment of FlgJ, a putative flagellar rod cap protein from Salmonella

    International Nuclear Information System (INIS)

    Kikuchi, Yuki; Matsunami, Hideyuki; Yamane, Midori; Imada, Katsumi; Namba, Keiichi

    2008-01-01

    A C-terminal fragment of Salmonella FlgJ, FlgJ 120–316 , which has peptidoglycan-hydrolysing activity, has been overproduced, purified and crystallized and the crystals have been characterized by X-ray diffraction. The formation of the bacterial flagellar axial structure, including the filament, the hook and the rod, requires the attachment of a cap complex to the distal end of the growing structure. Because the rod penetrates the peptidoglycan (PG) layer, the rod cap complex is thought to have PG-hydrolyzing activity. FlgJ is a putative rod cap protein whose C-terminal region shows sequence similarity to known muramidases. In this study, FlgJ 120–316 , a C-terminal fragment of FlgJ which contains the muramidase region, was overproduced, purified and crystallized. Crystals were obtained by the sitting-drop vapour-diffusion technique using PEG 3350 as a crystallizing agent and belonged to the orthorhombic space group P2 1 2 1 2 1 , with unit-cell parameters a = 38.8, b = 43.9, c = 108.5 Å. Anomalous difference Patterson maps calculated from the diffraction data set of a selenomethionine-labelled crystal showed significant peaks in the Harker sections, indicating that the data were suitable for structure determination

  1. Chagas' disease: IgG isotypes against cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens of Trypanosoma cruzi in chronic Chagasic patients.

    Science.gov (United States)

    Verçosa, A F A; Lorena, V M B; Carvalho, C L; Melo, M F A D; Cavalcanti, M G A; Silva, E D; Ferreira, A G P; Pereira, V R A; Souza, W V; Gomes, Y M

    2007-01-01

    The wide range of clinical Chagas' disease manifestations, of which heart involvement is the most significant, because of its characteristics, frequency and consequences, and lack of treatment and cure, justify research in this area. Specific immunoglobulin G (IgG) antibody subclasses have been associated with human Chagas' disease. Thus, in this study, the profile of IgG subclasses against cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive T. cruzi-specific antigens was correlated with cardiac (CARD, n=33), cardiodigestive (CD, n=7), and indeterminate (IND, n=20) forms of Chagas' disease by indirect enzyme-linked immunosorbent assay (ELISA). IgG subclasses were detected in almost all Chagas patients studied. Nevertheless, only specific IgG2 isotype FRA was found with a significant statistical difference in CARD patients when compared to IND patients. This result suggests the potential use of this isotype for prognostic purposes, for monitoring the progression of chronic Chagas' disease, and for predicting the risk of CARD damage. This is important information, as it could help physicians to evaluate and manage the treatment of their patients. However, a follow-up study is necessary to confirm our result. (c) 2007 Wiley-Liss, Inc.

  2. Modeling Induction Motor Imbalances

    DEFF Research Database (Denmark)

    Armah, Kabenla; Jouffroy, Jerome; Duggen, Lars

    2016-01-01

    This paper gives a study into the development of a generalized model for a three-phase induction motor that offers flexibility of simulating balanced and unbalanced parameter scenarios. By analyzing the interaction of forces within the motor, we achieve our main objective of deriving the system d...

  3. Artificial molecular motors

    NARCIS (Netherlands)

    Kassem, Salma; van Leeuwen, Thomas; Lubbe, Anouk S.; Wilson, Miriam R.; Feringa, Ben L.; Leigh, David A.

    2017-01-01

    Motor proteins are nature's solution for directing movement at the molecular level. The field of artificial molecular motors takes inspiration from these tiny but powerful machines. Although directional motion on the nanoscale performed by synthetic molecular machines is a relatively new

  4. To What Extent Can Motor Imagery Replace Motor Execution While Learning a Fine Motor Skill?

    NARCIS (Netherlands)

    Sobierajewicz, Jagna; Szarkiewicz, Sylwia; Prekoracka-Krawczyk, Anna; Jaskowski, Wojciech; van der Lubbe, Robert Henricus Johannes

    2016-01-01

    Motor imagery is generally thought to share common mechanisms with motor execution. In the present study, we examined to what extent learning a fine motor skill by motor imagery may substitute physical practice. Learning effects were assessed by manipulating the proportion of motor execution and

  5. Higher Efficiency HVAC Motors

    Energy Technology Data Exchange (ETDEWEB)

    Flynn, Charles Joseph [QM Power, Inc., Kansas City, MO (United States)

    2018-02-13

    The objective of this project was to design and build a cost competitive, more efficient heating, ventilation, and air conditioning (HVAC) motor than what is currently available on the market. Though different potential motor architectures among QMP’s primary technology platforms were investigated and evaluated, including through the building of numerous prototypes, the project ultimately focused on scaling up QM Power, Inc.’s (QMP) Q-Sync permanent magnet synchronous motors from available sub-fractional horsepower (HP) sizes for commercial refrigeration fan applications to larger fractional horsepower sizes appropriate for HVAC applications, and to add multi-speed functionality. The more specific goal became the research, design, development, and testing of a prototype 1/2 HP Q-Sync motor that has at least two operating speeds and 87% peak efficiency compared to incumbent electronically commutated motors (EC or ECM, also known as brushless direct current (DC) motors), the heretofore highest efficiency HVACR fan motor solution, at approximately 82% peak efficiency. The resulting motor prototype built achieved these goals, hitting 90% efficiency and .95 power factor at full load and speed, and 80% efficiency and .7 power factor at half speed. Q-Sync, developed in part through a DOE SBIR grant (Award # DE-SC0006311), is a novel, patented motor technology that improves on electronically commutated permanent magnet motors through an advanced electronic circuit technology. It allows a motor to “sync” with the alternating current (AC) power flow. It does so by eliminating the constant, wasteful power conversions from AC to DC and back to AC through the synthetic creation of a new AC wave on the primary circuit board (PCB) by a process called pulse width modulation (PWM; aka electronic commutation) that is incessantly required to sustain motor operation in an EC permanent magnet motor. The Q-Sync circuit improves the power factor of the motor by removing all

  6. Linear motor coil assembly and linear motor

    NARCIS (Netherlands)

    2009-01-01

    An ironless linear motor (5) comprising a magnet track (53) and a coil assembly (50) operating in cooperation with said magnet track (53) and having a plurality of concentrated multi-turn coils (31 a-f, 41 a-d, 51 a-k), wherein the end windings (31E) of the coils (31 a-f, 41 a-e) are substantially

  7. Spinal cord: motor neuron diseases.

    Science.gov (United States)

    Rezania, Kourosh; Roos, Raymond P

    2013-02-01

    Spinal cord motor neuron diseases affect lower motor neurons in the ventral horn. This article focuses on the most common spinal cord motor neuron disease, amyotrophic lateral sclerosis, which also affects upper motor neurons. Also discussed are other motor neuron diseases that only affect the lower motor neurons. Despite the identification of several genes associated with familial amyotrophic lateral sclerosis, the pathogenesis of this complex disease remains elusive. Copyright © 2013 Elsevier Inc. All rights reserved.

  8. [Children and motor competence].

    Science.gov (United States)

    Sigmundsson, H; Haga, M

    2000-10-20

    Recently, the topic of motor competence has figured prominently in the media. The claims made are many, but the research that support the statements is seldom cited. The aim of this review article is to address that deficiency by documenting what is really known about the motor competence of children. Motor competence not only allows children to carry out everyday practical tasks, but it is also an important determinant of their level of self-esteem and of their popularity and status in their peer group. While many studies have shown a significant correlation between motor problems and other problems in the social sphere, it has been difficult to establish causal relationships with any degree of confidence, as there appear to be several interactions which need to be taken into account. Research has shown that 6-10% of Norwegian children in the 7 to 10 year age group have a motor competence well below the norm. It is unusual for motor problems to simply disappear over time. In the absence of intervention the syndrome is likely to continue to manifest itself. More recent research points to some of the circularity in this causal network, children with motor problems having been shown to be less physically active than their peers. In a larger health perspective this in itself can have very serious consequences for the child.

  9. Soluble components of the flagellar export apparatus, FliI, FliJ, and FliH, do not deliver flagellin, the major filament protein, from the cytosol to the export gate.

    Science.gov (United States)

    Sajó, Ráchel; Liliom, Károly; Muskotál, Adél; Klein, Agnes; Závodszky, Péter; Vonderviszt, Ferenc; Dobó, József

    2014-11-01

    Flagella, the locomotion organelles of bacteria, extend from the cytoplasm to the cell exterior. External flagellar proteins are synthesized in the cytoplasm and exported by the flagellar type III secretion system. Soluble components of the flagellar export apparatus, FliI, FliH, and FliJ, have been implicated to carry late export substrates in complex with their cognate chaperones from the cytoplasm to the export gate. The importance of the soluble components in the delivery of the three minor late substrates FlgK, FlgL (hook-filament junction) and FliD (filament-cap) has been convincingly demonstrated, but their role in the transport of the major filament component flagellin (FliC) is still unclear. We have used continuous ATPase activity measurements and quartz crystal microbalance (QCM) studies to characterize interactions between the soluble export components and flagellin or the FliC:FliS substrate-chaperone complex. As controls, interactions between soluble export component pairs were characterized providing Kd values. FliC or FliC:FliS did not influence the ATPase activity of FliI alone or in complex with FliH and/or FliJ suggesting lack of interaction in solution. Immobilized FliI, FliH, or FliJ did not interact with FliC or FliC:FliS detected by QCM. The lack of interaction in the fluid phase between FliC or FliC:FliS and the soluble export components, in particular with the ATPase FliI, suggests that cells use different mechanisms for the export of late minor substrates, and the major substrate, FliC. It seems that the abundantly produced flagellin does not require the assistance of the soluble export components to efficiently reach the export gate. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. The non-flagellar type III secretion system evolved from the bacterial flagellum and diversified into host-cell adapted systems.

    Directory of Open Access Journals (Sweden)

    Sophie S Abby

    2012-09-01

    Full Text Available Type 3 secretion systems (T3SSs are essential components of two complex bacterial machineries: the flagellum, which drives cell motility, and the non-flagellar T3SS (NF-T3SS, which delivers effectors into eukaryotic cells. Yet the origin, specialization, and diversification of these machineries remained unclear. We developed computational tools to identify homologous components of the two systems and to discriminate between them. Our analysis of >1,000 genomes identified 921 T3SSs, including 222 NF-T3SSs. Phylogenomic and comparative analyses of these systems argue that the NF-T3SS arose from an exaptation of the flagellum, i.e. the recruitment of part of the flagellum structure for the evolution of the new protein delivery function. This reconstructed chronology of the exaptation process proceeded in at least two steps. An intermediate ancestral form of NF-T3SS, whose descendants still exist in Myxococcales, lacked elements that are essential for motility and included a subset of NF-T3SS features. We argue that this ancestral version was involved in protein translocation. A second major step in the evolution of NF-T3SSs occurred via recruitment of secretins to the NF-T3SS, an event that occurred at least three times from different systems. In rhizobiales, a partial homologous gene replacement of the secretin resulted in two genes of complementary function. Acquisition of a secretin was followed by the rapid adaptation of the resulting NF-T3SSs to multiple, distinct eukaryotic cell envelopes where they became key in parasitic and mutualistic associations between prokaryotes and eukaryotes. Our work elucidates major steps of the evolutionary scenario leading to extant NF-T3SSs. It demonstrates how molecular evolution can convert one complex molecular machine into a second, equally complex machine by successive deletions, innovations, and recruitment from other molecular systems.

  11. Motor Vehicle Safety

    Science.gov (United States)

    ... these crashes is one part of motor vehicle safety. Here are some things you can do to ... speed or drive aggressively Don't drive impaired Safety also involves being aware of others. Share the ...

  12. Motor Carrier Crash Data -

    Data.gov (United States)

    Department of Transportation — Contains data on large trucks and buses involved in Federally reportable crashes as per Title 49 U.S.C. Part 390.5 (crashes involving a commercial motor vehicle, and...

  13. High Power Density Motors

    Science.gov (United States)

    Kascak, Daniel J.

    2004-01-01

    With the growing concerns of global warming, the need for pollution-free vehicles is ever increasing. Pollution-free flight is one of NASA's goals for the 21" Century. , One method of approaching that goal is hydrogen-fueled aircraft that use fuel cells or turbo- generators to develop electric power that can drive electric motors that turn the aircraft's propulsive fans or propellers. Hydrogen fuel would likely be carried as a liquid, stored in tanks at its boiling point of 20.5 K (-422.5 F). Conventional electric motors, however, are far too heavy (for a given horsepower) to use on aircraft. Fortunately the liquid hydrogen fuel can provide essentially free refrigeration that can be used to cool the windings of motors before the hydrogen is used for fuel. Either High Temperature Superconductors (HTS) or high purity metals such as copper or aluminum may be used in the motor windings. Superconductors have essentially zero electrical resistance to steady current. The electrical resistance of high purity aluminum or copper near liquid hydrogen temperature can be l/lOO* or less of the room temperature resistance. These conductors could provide higher motor efficiency than normal room-temperature motors achieve. But much more importantly, these conductors can carry ten to a hundred times more current than copper conductors do in normal motors operating at room temperature. This is a consequence of the low electrical resistance and of good heat transfer coefficients in boiling LH2. Thus the conductors can produce higher magnetic field strengths and consequently higher motor torque and power. Designs, analysis and actual cryogenic motor tests show that such cryogenic motors could produce three or more times as much power per unit weight as turbine engines can, whereas conventional motors produce only 1/5 as much power per weight as turbine engines. This summer work has been done with Litz wire to maximize the current density. The current is limited by the amount of heat it

  14. Split-phase motor running as capacitor starts motor and as capacitor run motor

    Directory of Open Access Journals (Sweden)

    Yahaya Asizehi ENESI

    2016-07-01

    Full Text Available In this paper, the input parameters of a single phase split-phase induction motor is taken to investigate and to study the output performance characteristics of capacitor start and capacitor run induction motor. The value of these input parameters are used in the design characteristics of capacitor run and capacitor start motor with each motor connected to rated or standard capacitor in series with auxiliary winding or starting winding respectively for the normal operational condition. The magnitude of capacitor that will develop maximum torque in capacitor start motor and capacitor run motor are investigated and determined by simulation. Each of these capacitors is connected to the auxiliary winding of split-phase motor thereby transforming it into capacitor start or capacitor run motor. The starting current and starting torque of the split-phase motor (SPM, capacitor run motor (CRM and capacitor star motor (CSM are compared for their suitability in their operational performance and applications.

  15. Electrodynamic linear motor

    Energy Technology Data Exchange (ETDEWEB)

    Munehiro, H

    1980-05-29

    When driving the carriage of a printer through a rotating motor, there are problems regarding the limited accuracy of the carriage position due to rotation or contraction and ageing of the cable. In order to solve the problem, a direct drive system was proposed, in which the printer carriage is driven by a linear motor. If one wants to keep the motor circuit of such a motor compact, then the magnetic flux density in the air gap must be reduced or the motor travel must be reduced. It is the purpose of this invention to create an electrodynamic linear motor, which on the one hand is compact and light and on the other hand has a relatively high constant force over a large travel. The invention is characterised by the fact that magnetic fields of alternating polarity are generated at equal intervals in the magnetic field, and that the coil arrangement has 2 adjacent coils, whose size corresponds to half the length of each magnetic pole. A logic circuit is provided to select one of the two coils and to determine the direction of the current depending on the signals of a magnetic field sensor on the coil arrangement.

  16. Markov process of muscle motors

    International Nuclear Information System (INIS)

    Kondratiev, Yu; Pechersky, E; Pirogov, S

    2008-01-01

    We study a Markov random process describing muscle molecular motor behaviour. Every motor is either bound up with a thin filament or unbound. In the bound state the motor creates a force proportional to its displacement from the neutral position. In both states the motor spends an exponential time depending on the state. The thin filament moves at a velocity proportional to the average of all displacements of all motors. We assume that the time which a motor stays in the bound state does not depend on its displacement. Then one can find an exact solution of a nonlinear equation appearing in the limit of an infinite number of motors

  17. Electric vehicle motors and controllers

    Science.gov (United States)

    Secunde, R. R.

    1981-01-01

    Improved and advanced components being developed include electronically commutated permanent magnet motors of both drum and disk configuration, an unconventional brush commutated motor, and ac induction motors and various controllers. Test results on developmental motors, controllers, and combinations thereof indicate that efficiencies of 90% and higher for individual components, and 80% to 90% for motor/controller combinations can be obtained at rated power. The simplicity of the developmental motors and the potential for ultimately low cost electronics indicate that one or more of these approaches to electric vehicle propulsion may eventually displace presently used controllers and brush commutated dc motors.

  18. A versatile stepping motor controller for systems with many motors

    International Nuclear Information System (INIS)

    Feng, S.K.; Siddons, D.P.

    1989-01-01

    A versatile system for controlling beamlines or complex experimental setups is described. The system as currently configured can control up to 32 motors, with all motors capable of full speed operation concurrently. There are 2 limit switch inputs for each motor, and a further input to accept a reference position marker. The motors can be controlled via a front panel keyboard with display, or by a host computer over an IEEE-488 interface. Both methods can be used together if required. There is an ''emergency stop'' key on the front panel keyboard to stop the motion of all motors without losing track of the motors' position. 3 refs., 4 figs., 1 tab

  19. Flood-proof motors

    Energy Technology Data Exchange (ETDEWEB)

    Schmitt, Marcus [AREVA NP GmbH, Erlangen (Germany)

    2013-07-01

    Even before the Fukushima event occurred some German nuclear power plants (NPP) have considered flooding scenarios. As a result of one of these studies, AREVA performed an upgrade project in NPP Isar 1 with flood-proof motors as a replacement of existing air-cooled low-voltage and high-voltage motors of the emergency cooling chain. After the Fukushima event, in which the cooling chains failed, the topic flood-proof equipment gets more and more into focus. This compact will introduce different kinds of flood-proof electrical motors which are currently installed or planned for installation into NPPs over the world. Moreover the process of qualification, as it was performed during the project in NPP Isar 1, will be shown. (orig.)

  20. Advanced AC Motor Control

    Energy Technology Data Exchange (ETDEWEB)

    Kazmierkowski, M.P. [Institute of Control and Industrial Electronics, Warsaw University of Technology, Warszawa (Poland)

    1997-12-31

    In this paper a review of control methods for high performance PWM inverter-fed induction motor drives is presented. Starting from the description of an induction motor by the help of the space vectors, three basic control strategic are discussed. As first, the most popular Field Oriented Control (FOC) is described. Secondly, the Direct Torque and Flux vector Control (DTFC) method, which - in contrast to FOC - depart from idea of coordinate transformation and analogy with DC motor, is briefly characterized. The last group is based on Feedback Linearization Control (FLC) and can be easy combined with sliding mode control. The simulation and experimental oscillograms that illustrate the performance of the discussed control strategies are shown. (orig.) 35 refs.

  1. Flood-proof motors

    International Nuclear Information System (INIS)

    Schmitt, Marcus

    2013-01-01

    Even before the Fukushima event occurred some German nuclear power plants (NPP) have considered flooding scenarios. As a result of one of these studies, AREVA performed an upgrade project in NPP Isar 1 with flood-proof motors as a replacement of existing air-cooled low-voltage and high-voltage motors of the emergency cooling chain. After the Fukushima event, in which the cooling chains failed, the topic flood-proof equipment gets more and more into focus. This compact will introduce different kinds of flood-proof electrical motors which are currently installed or planned for installation into NPPs over the world. Moreover the process of qualification, as it was performed during the project in NPP Isar 1, will be shown. (orig.)

  2. Mechanical design of electric motors

    CERN Document Server

    Tong, Wei

    2014-01-01

    Rapid increases in energy consumption and emphasis on environmental protection have posed challenges for the motor industry, as has the design and manufacture of highly efficient, reliable, cost-effective, energy-saving, quiet, precisely controlled, and long-lasting electric motors.Suitable for motor designers, engineers, and manufacturers, as well as maintenance personnel, undergraduate and graduate students, and academic researchers, Mechanical Design of Electric Motors provides in-depth knowledge of state-of-the-art design methods and developments of electric motors. From motor classificati

  3. Transformers and motors

    CERN Document Server

    Shultz, George

    1991-01-01

    Transformers and Motors is an in-depth technical reference which was originally written for the National Joint Apprenticeship Training Committee to train apprentice and journeymen electricians. This book provides detailed information for equipment installation and covers equipment maintenance and repair. The book also includes troubleshooting and replacement guidelines, and it contains a minimum of theory and math.In this easy-to-understand, practical sourcebook, you'll discover:* Explanations of the fundamental concepts of transformers and motors* Transformer connections and d

  4. Linear induction motor

    International Nuclear Information System (INIS)

    Barkman, W.E.; Adams, W.Q.; Berrier, B.R.

    1978-01-01

    A linear induction motor has been operated on a test bed with a feedback pulse resolution of 5 nm (0.2 μin). Slewing tests with this slide drive have shown positioning errors less than or equal to 33 nm (1.3 μin) at feedrates between 0 and 25.4 mm/min (0-1 ipm). A 0.86-m (34-in)-stroke linear motor is being investigated, using the SPACO machine as a test bed. Initial results were encouraging, and work is continuing to optimize the servosystem compensation

  5. Human spinal motor control

    DEFF Research Database (Denmark)

    Nielsen, Jens Bo

    2016-01-01

    Human studies in the past three decades have provided us with an emerging understanding of how cortical and spinal networks collaborate to ensure the vast repertoire of human behaviors. We differ from other animals in having direct cortical connections to spinal motoneurons, which bypass spinal...... the central motor command by opening or closing sensory feedback pathways. In the future, human studies of spinal motor control, in close collaboration with animal studies on the molecular biology of the spinal cord, will continue to document the neural basis for human behavior. Expected final online...

  6. Electrodynamic ratchet motor.

    Science.gov (United States)

    Lim, Jiufu; Sader, John E; Mulvaney, Paul

    2009-03-01

    Brownian ratchets produce directed motion through rectification of thermal fluctuations and have been used for separation processes and colloidal transport. We propose a flashing ratchet motor that enables the transduction of electrical energy into rotary micromechanical work. This is achieved through torque generation provided by boundary shaping of equipotential surfaces. The present device contrasts to previous implementations that focus on translational motion. Stochastic simulations elucidate the performance characteristics of this device as a function of its geometry. Miniaturization to nanoscale dimensions yields rotational speeds in excess of 1 kHz, which is comparable to biomolecular motors of similar size.

  7. The effect of induction motor shaft diameter on motor performance

    Directory of Open Access Journals (Sweden)

    Asım Gökhan Yetgin

    2017-10-01

    Full Text Available Induction motors are used in many areas from the past to the present and in different fields with the development of technology has continued to be used. It is obvious that induction motors as an improvement to the efficiency in terms of energy saving would cause great benefit. In that context, induction motor manufacturers and designers are constantly trying out new methods to improve motor performance and efficiency. In this study, what would be the optimum diameter of the shaft in order to increase the efficiency of the induction motor were investigated. In the study, 5.5 kW, 7.5 kW and 11 kW motors analyzes were also performed. Obtained shaft diameter values were compared with the manufacturer values. In addition, critical points such as the magnetic flux values, weight values and performances of the motors were examined and optimal shaft diameter values for each motor have been determined.

  8. HTSL massive motor. Project: Motor field calculation. Final report

    International Nuclear Information System (INIS)

    Gutt, H.J.; Gruener, A.

    2003-01-01

    HTS motors up to 300 kW were to be developed and optimized. For this, specific calculation methods were enhanced to include superconducting rotor types (hysteresis, reluctance and permanent magnet HTS rotors). The experiments were carried out in a SHM70-45 hysteresis motor. It was shown how static and dynamic trapped field magnetisation of the rotor with YBCO rings will increase flux in the air gap motor, increasing the motor capacity to twice its original level. (orig.) [de

  9. Stepping Motor - Hydraulic Motor Servo Drives for an NC Milling ...

    African Journals Online (AJOL)

    In this paper the retrofit design of the control system of an NC milling machine with a stepping motor and stepping motor - actuated hydraulic motor servo mechanism on the machines X-axis is described. The servo designed in the course of this study was tested practically and shown to be linear - the velocity following errors ...

  10. Thiokol Solid Rocket Motors

    Science.gov (United States)

    Graves, S. R.

    2000-01-01

    This paper presents viewgraphs on thiokol solid rocket motors. The topics include: 1) Communications; 2) Military and government intelligence; 3) Positioning satellites; 4) Remote sensing; 5) Space burial; 6) Science; 7) Space manufacturing; 8) Advertising; 9) Space rescue space debris management; 10) Space tourism; 11) Space settlements; 12) Hazardous waste disposal; 13) Extraterrestrial resources; 14) Fast package delivery; and 15) Space utilities.

  11. Reciprocating Linear Electric Motor

    Science.gov (United States)

    Goldowsky, M. P.

    1984-01-01

    Features include structural simplicity and good force/displacement characteristics. Reciprocating motor has simple, rugged construction, relatively low reciprocating weight, improved power delivery, and improved force control. Wear reduced by use of magnetic bearings. Intended to provide drivers for long-lived Stirling-cycle cryogenic refrigerators, concept has less exotic applications, such as fuel pumps.

  12. Switched reluctance motor drives

    Indian Academy of Sciences (India)

    Davis RM, Ray WF, Blake RJ 1981 Inverter drive for switched reluctance: circuits and component ratings. Inst. Elec. Eng. Proc. B128: 126-136. Ehsani M. 1991 Position Sensor elimination technique for the switched reluctance motor drive. US Patent No. 5,072,166. Ehsani M, Ramani K R 1993 Direct control strategies based ...

  13. Motor Incoordination in ADHD

    Directory of Open Access Journals (Sweden)

    J Gordon Millichap

    2004-07-01

    Full Text Available The relationship between motor performance, attention deficit, impulsiveness, and hyperactivity in 42 school-aged children with ADHD (36 males, 6 females; mean age 8 years 2 months; range 6-11 years was studied at National Taiwan University, Taipei, Taiwan.

  14. Deafness and motor abilities level

    Directory of Open Access Journals (Sweden)

    A Zwierzchowska

    2008-09-01

    Full Text Available The audition injury hinders some motor motions and the organised coordination at the higher level and may be a cause of disturbances and disorder in some motor abilities adoption. It was assumed that deafness including its aetiology and injury mechanism may significantly influence the motor development of human being. The study aimed in checking if the deafness, as a result of various unfavourable factors, determines the motor development of children and youngsters. Consequently the dependency between qualitative features i.e.: signed motor level and aetiology, audition injury mechanism and the deafness degree was examined. The mechanism and aetiology of hearing correlated with the motor abilities displayed statistically significant dependencies in few motor trials only. Revealed correlations regarded mostly the coordination trials excluding the flexibility one. Statistically significant dependencies between the audition diminution and the motor abilities level were not found.

  15. MOTORIC STIMULATION RELATED TO FINE MOTORIC DEVELOPMENT ON CHILD

    Directory of Open Access Journals (Sweden)

    Mira Triharini

    2017-07-01

    Full Text Available Introduction: Motor developmental stimulation is an activity undertaken to stimulate the children basic skills and so they can grow and develop optimally. Children who obtain a direct stimulus will grow faster than who get less stimulus. Mother’s behavior of stimulation is very important for children, it is considering as the basic needs of children and it must be fulfilled. Providing good stimulation could optimize fine motor development in children. The purpose of this study was to analyze mother’s behavior about motor stimulation with fine motor development in toddler age 4-5 years old. Method: Design have been  used in this study was cross sectional. Population were mothers and their toddler in Group A of Dharma Wanita Persatuan Driyorejo Gresik Preschool. Sample were 51 respondents recruited by using purposive sampling technique according to inclusion and exclusion criteria. The independent variable was mother’s behavior about motor stimulation whereas dependent variable was fine motor development in toddler. The data were collected using questionnaire and conducting observation on fine motor development based on Denver Development Screening Test (DDST. Data then analyzed using Spearman Rho (r test to find relation between mother’s behaviors about stimulation motor on their toddler fine motor development. Result: Results  of this study showed that there were correlations between mother’s knowledge and fine motor development in toddler (p=0.000, between mother’s attitude and fine motor development in toddler (p=0.000, and between mother’s actions and fine motor development in toddler (p=0.000. Analysis: In sort study found that there were relation between fine motor development and mother’s behavior. Discussion: Therefore mother’s behavior needed to be improved. Further research about stimulation motor and fine motor development aspects in toddler is required.

  16. Microprocessor controller for stepping motors

    International Nuclear Information System (INIS)

    Strait, B.G.; Thuot, M.E.

    1977-01-01

    A new concept for digital computer control of multiple stepping motors which operate in a severe electromagnetic pulse environment is presented. The motors position mirrors in the beam-alignment system of a 100-kJ CO 2 laser. An asynchronous communications channel of a computer is used to send coded messages, containing the motor address and stepping-command information, to the stepping-motor controller in a bit serial format over a fiber-optics communications link. The addressed controller responds by transmitting to the computer its address and other motor information, thus confirming the received message. Each controller is capable of controlling three stepping motors. The controller contains the fiber-optics interface, a microprocessor, and the stepping-motor driven circuits. The microprocessor program, which resides in an EPROM, decodes the received messages, transmits responses, performs the stepping-motor sequence logic, maintains motor-position information, and monitors the motor's reference switch. For multiple stepping-motor application, the controllers are connected in a daisy chain providing control of many motors from one asynchronous communications channel of the computer

  17. Multiple stage miniature stepping motor

    International Nuclear Information System (INIS)

    Niven, W.A.; Shikany, S.D.; Shira, M.L.

    1981-01-01

    A stepping motor comprising a plurality of stages which may be selectively activated to effect stepping movement of the motor, and which are mounted along a common rotor shaft to achieve considerable reduction in motor size and minimum diameter, whereby sequential activation of the stages results in successive rotor steps with direction being determined by the particular activating sequence followed

  18. Experiments with a DC Motor

    Science.gov (United States)

    Kraftmakher, Yaakov

    2010-01-01

    Experiments with an electric motor provide good opportunity to demonstrate some basic laws of electricity and magnetism. The aim of the experiments with a low-power dc motor is to show how the motor approaches its steady rotation and how its torque, mechanical power and efficiency depend on the rotation velocity. The tight relationship between the…

  19. Fundamental Principles underlying Motor Reflexes

    NARCIS (Netherlands)

    K. Zhou (Kuikui)

    2017-01-01

    markdownabstractThe cerebellum has been suggested to be involved in motor control ever since the early 19th century. The motor control ranges from timing and strength of simple reflexes to multiple joint/limb coordination and complex motor sequence acquisition. The current thesis discusses the

  20. Motor Vehicle Theft. Special Report.

    Science.gov (United States)

    Harlow, Caroline Wolf

    Thirteen years of data from the National Crime Survey were analyzed to examine the characteristics of motor vehicle theft, to identify trends during the past 13 years, and to determine who are most likely to be victims of motor vehicle theft. All motor vehicle thefts reported to the National Crime Survey from 1973 through 1985 were examined.…

  1. Segmented motor drive - with multi-phase induction motor

    DEFF Research Database (Denmark)

    Bendixen, Flemming Buus

    of the induction motor is set up. The model is able to calculate dynamical electric, magnetic and mechanic state variables, but initially it is used to calculate static characteristics in motors with different number of phases and different voltage supply shapes. This analysis show i.e. that the efficiency....... The multi-phase motor is selected for further analysis. The project is limited to examine if increasing the number of phases can improve the characteristics for induction motor drives. In the literature it is demonstrated that torque production in a six-phase motor can be increased, if a 3rd harmonic......This PhD project commences in modulation of motor drives, i.e. having the advantage of reducing the number of variants and improves the system reliability at error situations. Four different motor drive topologies with modular construction as common denominator are compared on a general level...

  2. Variation in motor output and motor performance in a centrally generated motor pattern

    Science.gov (United States)

    Norris, Brian J.; Doloc-Mihu, Anca; Calabrese, Ronald L.

    2014-01-01

    Central pattern generators (CPGs) produce motor patterns that ultimately drive motor outputs. We studied how functional motor performance is achieved, specifically, whether the variation seen in motor patterns is reflected in motor performance and whether fictive motor patterns differ from those in vivo. We used the leech heartbeat system in which a bilaterally symmetrical CPG coordinates segmental heart motor neurons and two segmented heart tubes into two mutually exclusive coordination modes: rear-to-front peristaltic on one side and nearly synchronous on the other, with regular side-to-side switches. We assessed individual variability of the motor pattern and the beat pattern in vivo. To quantify the beat pattern we imaged intact adults. To quantify the phase relations between motor neurons and heart constrictions we recorded extracellularly from two heart motor neurons and movement from the corresponding heart segments in minimally dissected leeches. Variation in the motor pattern was reflected in motor performance only in the peristaltic mode, where larger intersegmental phase differences in the motor neurons resulted in larger phase differences between heart constrictions. Fictive motor patterns differed from those in vivo only in the synchronous mode, where intersegmental phase differences in vivo had a larger front-to-rear bias and were more constrained. Additionally, load-influenced constriction timing might explain the amplification of the phase differences between heart segments in the peristaltic mode and the higher variability in motor output due to body shape assumed in this soft-bodied animal. The motor pattern determines the beat pattern, peristaltic or synchronous, but heart mechanics influence the phase relations achieved. PMID:24717348

  3. Split-phase motor running as capacitor starts motor and as capacitor run motor

    OpenAIRE

    Yahaya Asizehi ENESI; Jacob TSADO; Mark NWOHU; Usman Abraham USMAN; Odu Ayo IMORU

    2016-01-01

    In this paper, the input parameters of a single phase split-phase induction motor is taken to investigate and to study the output performance characteristics of capacitor start and capacitor run induction motor. The value of these input parameters are used in the design characteristics of capacitor run and capacitor start motor with each motor connected to rated or standard capacitor in series with auxiliary winding or starting winding respectively for the normal operational condition. The ma...

  4. Acute exercise improves motor memory

    DEFF Research Database (Denmark)

    Skriver, Kasper Christen; Roig, Marc; Lundbye-Jensen, Jesper

    2014-01-01

    We have recently shown that a single bout of acute cardiovascular exercise improves motor skill learning through an optimization of long-term motor memory. Here we expand this previous finding, to explore potential exercise-related biomarkers and their association with measures of motor memory...... practice whereas lactate correlated with better retention 1 hour as well as 24 hours and 7 days after practice. Thus, improvements in motor skill acquisition and retention induced by acute cardiovascular exercise are associated with increased concentrations of biomarkers involved in memory and learning...... processes. More mechanistic studies are required to elucidate the specific role of each biomarker in the formation of motor memory....

  5. Dynamically Timed Electric Motor

    Science.gov (United States)

    Casper, Ann M. (Inventor)

    1997-01-01

    A brushless DC motor including a housing having an end cap secured thereto. The housing encloses a rotor. a stator and a rotationally displaceable commutation board having sensors secured thereon and spaced around the periphery of the rotor. An external rotational force is applied to the commutation board for displacement of the sensors to various positions whereby varying feedback signals are generated by the positioning of the sensors relative to the rotating rotor. The commutation board is secured in a fixed position in response to feedback signals indicative of optimum sensor position being determined. The rotation of the commutation board and the securing of the sensors in the desired fixed position is accomplished without requiring the removal of the end cap and with the DC motor operating.

  6. TFTR Motor Generator

    International Nuclear Information System (INIS)

    Murray, J.G.; Bronner, G.; Horton, M.

    1977-01-01

    A general description is given of 475 MVA pulsed motor generators for TFTR at Princeton Plasma Physics Laboratory. Two identical generators operating in parallel are capable of supplying 950 MVA for an equivalent square pulse of 6.77 seconds and 4,500 MJ at 0.7 power factor to provide the energy for the pulsed electrical coils and heating system for TFTR. The description includes the operational features of the 15,000 HP wound rotor motors driving each generator with its starting equipment and cycloconverter for controlling speed, power factor, and regulating line voltage during load pulsing where the generator speed changes from 87.5 to 60 Hz frequency variation to provide the 4,500 MJ or energy. The special design characteristics such as fatigue stress calculations for 10 6 cycles of operation, forcing factor on exciter to provide regulation, and low generator impedance are reviewed

  7. Libert-E Motor

    Science.gov (United States)

    Sieloff, Susan F.; Kinnunen, Raymond; Chevarley, Joseph

    2011-01-01

    Kei Yun Wong has big dreams. She has been entrusted with the United States launch of Libert-E Motor, a new line of Chinese-manufactured electric scooters. With only $750,000 of her original budget of $3 million left, she needs to make sure that the launch succeeds, as it represents the initial step in her desire to create the first Chinese global…

  8. 350 KVA motor generators

    CERN Multimedia

    CERN PhotoLab

    1974-01-01

    Each logic circuit in the central computers consumes only a fraction of a watt: however, the final load constituted by many such circuits plus peripheral equipment is nearly half a million watts. Shown here are two 350 KVA motor generators used to convert 50 Hz mains to 60 Hz (US standard). Flywheels on the M.G. shafts remove power dropouts of up to 0.5 s.

  9. Motor Fuel Excise Taxes

    Energy Technology Data Exchange (ETDEWEB)

    2015-09-01

    A new report from the National Renewable Energy Laboratory (NREL) explores the role of alternative fuels and energy efficient vehicles in motor fuel taxes. Throughout the United States, it is common practice for federal, state, and local governments to tax motor fuels on a per gallon basis to fund construction and maintenance of our transportation infrastructure. In recent years, however, expenses have outpaced revenues creating substantial funding shortfalls that have required supplemental funding sources. While rising infrastructure costs and the decreasing purchasing power of the gas tax are significant factors contributing to the shortfall, the increased use of alternative fuels and more stringent fuel economy standards are also exacerbating revenue shortfalls. The current dynamic places vehicle efficiency and petroleum use reduction polices at direct odds with policies promoting robust transportation infrastructure. Understanding the energy, transportation, and environmental tradeoffs of motor fuel tax policies can be complicated, but recent experiences at the state level are helping policymakers align their energy and environmental priorities with highway funding requirements.

  10. Motor car driving; Kraftfahrzeugfuehrung

    Energy Technology Data Exchange (ETDEWEB)

    Juergensohn, T. [Technische Univ. Berlin (Germany). ISS-Fahrzeugtechnik; Timpe, K.P. (eds.) [Technische Univ. Berlin (DE). Zentrum Mensch-Maschine-Systeme (ZMMS)

    2001-07-01

    This is the first comprehensive book on motor car driving, i.e. all aspects of motor car technology that cannot be looked at separately from the needs, characteristics and limitations of the human driver. This includes ergonomics as well as the design of the driver interface in consideration of the findings of cognitive science, problems of driving simulation in the context of simulation of technical systems, problems relating to optimal car automation up to traffic psychology. The book is in honour of Prof. Dr. Willumeit who died in summer 2000. Prof. Willumeit was one of the few scientists in Germany who had been an expert on all aspects of motor car driving for many years. [German] Erstmalig wird das Thema der Fahrzeugfuehrung geschlossen dargestellt. Die Thematik der 'Kraftfahrzeugfuehrung' umfasst in diesem Zusammenhang alle Aspekte der Kraftfahrzeugtechnik, die nicht isoliert von den Erfordernissen, Eigenschaften und Grenzen des menschlichen Fahrers betrachtet werden koennen. Dies beinhaltet u.a. Probleme der Ergonomie, aber auch Fragen nach einer kognitionswissenschaftlich unterstuetzten Schnittstellengestaltung, Fragen der Simulation des Fahrverhalten im Kontext der Simulation technischer Systeme oder Fragen einer optimalen Fahrzeugautomatisierung bis hin zu verkehrspsychologischen Aspekten. Das Buch ist als Gedenkband fuer Prof. Dr. Willumeit konzipiert, der im Sommer 2000 verstarb. Prof. Willumeit war einer der wenigen Wissenschaftler in Deutschland, der ueber viele Jahre diese Thematik der Kraftfahrzeugfuehrung in ihrer vollen Breite verfolgte. (orig.)

  11. Plasmid AZOBR_p1-borne fabG gene for putative 3-oxoacyl-[acyl-carrier protein] reductase is essential for proper assembly and work of the dual flagellar system in the alphaproteobacterium Azospirillum brasilense Sp245.

    Science.gov (United States)

    Filip'echeva, Yulia A; Shelud'ko, Andrei V; Prilipov, Alexei G; Burygin, Gennady L; Telesheva, Elizaveta M; Yevstigneyeva, Stella S; Chernyshova, Marina P; Petrova, Lilia P; Katsy, Elena I

    2018-02-01

    Azospirillum brasilense can swim and swarm owing to the activity of a constitutive polar flagellum (Fla) and inducible lateral flagella (Laf), respectively. Experimental data on the regulation of the Fla and Laf assembly in azospirilla are scarce. Here, the coding sequence (CDS) AZOBR_p1160043 (fabG1) for a putative 3-oxoacyl-[acyl-carrier protein (ACP)] reductase was found essential for the construction of both types of flagella. In an immotile leaky Fla - Laf - fabG1::Omegon-Km mutant, Sp245.1610, defects in flagellation and motility were fully complemented by expressing the CDS AZOBR_p1160043 from plasmid pRK415. When pRK415 with the cloned CDS AZOBR_p1160045 (fliC) for a putative 65.2 kDa Sp245 Fla flagellin was transferred into the Sp245.1610 cells, the bacteria also became able to assemble a motile single flagellum. Some cells, however, had unusual swimming behavior, probably because of the side location of the organelle. Although the assembly of Laf was not restored in Sp245.1610 (pRK415-p1160045), this strain was somewhat capable of swarming motility. We propose that the putative 3-oxoacyl-[ACP] reductase encoded by the CDS AZOBR_p1160043 plays a role in correct flagellar location in the cell envelope and (or) in flagellar modification(s), which are also required for the inducible construction of Laf and for proper swimming and swarming motility of A. brasilense Sp245.

  12. 76 FR 647 - Energy Conservation Program: Test Procedures for Electric Motors and Small Electric Motors

    Science.gov (United States)

    2011-01-05

    ... Electric Motors and Small Electric Motors; Proposed Rule #0;#0;Federal Register / Vol. 76, No. 3... Motors and Small Electric Motors AGENCY: Office of Energy Efficiency and Renewable Energy, Department of... motors and small electric motors, clarify the scope of energy conservation standards for electric motors...

  13. 46 CFR 111.70-3 - Motor controllers and motor-control centers.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Motor controllers and motor-control centers. 111.70-3... ELECTRIC SYSTEMS-GENERAL REQUIREMENTS Motor Circuits, Controllers, and Protection § 111.70-3 Motor controllers and motor-control centers. (a) General. The enclosure for each motor controller or motor-control...

  14. Performance Comparison between a Permanent Magnet Synchronous Motor and an Induction Motor as a Traction Motor for High Speed Train

    Science.gov (United States)

    Kondo, Minoru; Kawamura, Junya; Terauchi, Nobuo

    Performance tests are carried out to demonstrate the superiority of a permanent magnet synchronous motor to an induction motor as a traction motor for high-speed train. A prototype motor was manufactured by replacing the rotor of a conventional induction motor. The test results show that the permanent magnet motor is lighter, efficient and more silent than the induction motor because of the different rotor structure.

  15. Neurons other than motor neurons in motor neuron disease.

    Science.gov (United States)

    Ruffoli, Riccardo; Biagioni, Francesca; Busceti, Carla L; Gaglione, Anderson; Ryskalin, Larisa; Gambardella, Stefano; Frati, Alessandro; Fornai, Francesco

    2017-11-01

    Amyotrophic lateral sclerosis (ALS) is typically defined by a loss of motor neurons in the central nervous system. Accordingly, morphological analysis for decades considered motor neurons (in the cortex, brainstem and spinal cord) as the neuronal population selectively involved in ALS. Similarly, this was considered the pathological marker to score disease severity ex vivo both in patients and experimental models. However, the concept of non-autonomous motor neuron death was used recently to indicate the need for additional cell types to produce motor neuron death in ALS. This means that motor neuron loss occurs only when they are connected with other cell types. This concept originally emphasized the need for resident glia as well as non-resident inflammatory cells. Nowadays, the additional role of neurons other than motor neurons emerged in the scenario to induce non-autonomous motor neuron death. In fact, in ALS neurons diverse from motor neurons are involved. These cells play multiple roles in ALS: (i) they participate in the chain of events to produce motor neuron loss; (ii) they may even degenerate more than and before motor neurons. In the present manuscript evidence about multi-neuronal involvement in ALS patients and experimental models is discussed. Specific sub-classes of neurons in the whole spinal cord are reported either to degenerate or to trigger neuronal degeneration, thus portraying ALS as a whole spinal cord disorder rather than a disease affecting motor neurons solely. This is associated with a novel concept in motor neuron disease which recruits abnormal mechanisms of cell to cell communication.

  16. Comparison of capabilities of reluctance synchronous motor and induction motor

    International Nuclear Information System (INIS)

    Stumberger, Gorazd; Hadziselimovic, Miralem; Stumberger, Bojan; Miljavec, Damijan; Dolinar, Drago; Zagradisnik, Ivan

    2006-01-01

    This paper compares the capabilities of a reluctance synchronous motor (RSM) with those of an induction motor (IM). An RSM and IM were designed and made, with the same rated power and speed. They differ only in the rotor portion while their stators, housings and cooling systems are identical. The capabilities of both motors in a variable speed drive are evaluated by comparison of the results obtained by magnetically nonlinear models and by measurements

  17. Control of synchronous motors

    CERN Document Server

    Louis, Jean-Paul

    2013-01-01

    Synchronous motors are indubitably the most effective device to drive industrial production systems and robots with precision and rapidity. Their control law is thus critical for combining at the same time high productivity to reduced energy consummation. As far as possible, the control algorithms must exploit the properties of these actuators. Therefore, this work draws on well adapted models resulting from the Park's transformation, for both the most traditional machines with sinusoidal field distribution and for machines with non-sinusoidal field distribution which are more and more used in

  18. Electric Motors for Vehicle Propulsion

    OpenAIRE

    Larsson, Martin

    2014-01-01

    This work is intended to contribute with knowledge to the area of electic motorsfor propulsion in the vehicle industry. This is done by first studying the differentelectric motors available, the motors suitable for vehicle propulsion are then dividedinto four different types to be studied separately. These four types are thedirect current, induction, permanent magnet and switched reluctance motors. Thedesign and construction are then studied to understand how the different typesdiffer from ea...

  19. High efficiency motor selection handbook

    Science.gov (United States)

    McCoy, Gilbert A.; Litman, Todd; Douglass, John G.

    1990-10-01

    Substantial reductions in energy and operational costs can be achieved through the use of energy-efficient electric motors. A handbook was compiled to help industry identify opportunities for cost-effective application of these motors. It covers the economic and operational factors to be considered when motor purchase decisions are being made. Its audience includes plant managers, plant engineers, and others interested in energy management or preventative maintenance programs.

  20. Motor development of blind toddler

    OpenAIRE

    Likar, Petra

    2013-01-01

    For blind toddlers, development of motor skills enables possibilities for learning and exploring the environment. The purpose of this graduation thesis is to systematically mark the milestones in development of motor skills in blind toddlers, to establish different factors which affect this development, and to discover different ways for teachers for visually impaired and parents to encourage development of motor skills. It is typical of blind toddlers that they do not experience a wide varie...

  1. Motor fuel prices in Turkey

    International Nuclear Information System (INIS)

    Erdogdu, Erkan

    2014-01-01

    The world's most expensive motor fuel (gasoline, diesel and LPG) is sold most likely in the Republic of Turkey. This paper investigates the key issues related to the motor fuel prices in Turkey. First of all, the paper analyses the main reason behind high prices, namely motor fuel taxes in Turkey. Then, it estimates the elasticity of motor fuel demand in Turkey using an econometric analysis. The findings indicate that motor fuel demand in Turkey is quite inelastic and, therefore, not responsive to price increases caused by an increase in either pre-tax prices or taxes. Therefore, fuel market in Turkey is open to opportunistic behavior by firms (through excessive profits) and the government (through excessive taxes). Besides, the paper focuses on the impact of high motor fuel prices on road transport associated activities, including the pattern of passenger transportation, motorization rate, fuel use, total kilometers traveled and CO 2 emissions from road transportation. The impact of motor fuel prices on income distribution in Turkey and Turkish public opinion about high motor fuel prices are also among the subjects investigated in the course of the study. - Highlights: • The key issues (e.g. taxes) related to motor fuel prices in Turkey are explored. • Their impact on transport activities and income distribution is also investigated. • An econometric analysis is performed to estimate motor fuel demand in Turkey. • Motor fuel demand in Turkey is found to be quite inelastic. • Turkish fuel market is open to opportunistic behavior by firms and the government

  2. Motor of the future - superconducting

    International Nuclear Information System (INIS)

    Moen, Odd

    2001-01-01

    High-temperature superconductors count as the most innovative and future-oriented technology for electric motors. When these materials are used, the engine rating can be doubled and at the same time the losses halved while retaining the same size of construction. Siemens have recently developed a synchronous motor based on a high-temperature superconducting excitation winding. The rated power of the motor is 380 kW. The high-temperature superconductor that is used in this motor requires considerably less cooling outfit than low-temperature superconductors

  3. Overview of Bearingless Induction Motors

    Directory of Open Access Journals (Sweden)

    Xiaodong Sun

    2014-01-01

    Full Text Available Bearingless induction motors combining functions of both torque generation and noncontact magnetic suspension together have attracted more and more attention in the past decades due to their definite advantages of compactness, simple structure, less maintenance, no wear particles, high rotational speed, and so forth. This paper overviews the key technologies of the bearingless induction motors, with emphasis on motor topologies, mathematical models, and control strategies. Particularly, in the control issues, the vector control, independent control, direct torque control, nonlinear decoupling control, sensorless control, and so forth are investigated. In addition, several possible development trends of the bearingless induction motors are also discussed.

  4. Gap junctions and motor behavior

    DEFF Research Database (Denmark)

    Kiehn, Ole; Tresch, Matthew C.

    2002-01-01

    The production of any motor behavior requires coordinated activity in motor neurons and premotor networks. In vertebrates, this coordination is often assumed to take place through chemical synapses. Here we review recent data suggesting that electrical gap-junction coupling plays an important role...... in coordinating and generating motor outputs in embryonic and early postnatal life. Considering the recent demonstration of a prevalent expression of gap-junction proteins and gap-junction structures in the adult mammalian spinal cord, we suggest that neuronal gap-junction coupling might also contribute...... to the production of motor behavior in adult mammals....

  5. Design of an HTS motor

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Y; Pei, R; Hong, Z; Jiang, Q; Coombs, T A [Cambridge University engineering Department, Trumpington Street, Cambridge CB2 1PZ (United Kingdom)], E-mail: yj222@cam.ac.uk

    2008-02-01

    This paper gives a detailed description of the design of a high temperature superconducting (HTS) motor. The stator of the motor consists of six air cored HTS racetrack windings, together with an iron shield. The rotor is made of 80 superconducting YBCO pucks, which can be magnetized and equates to a four-pole permanent magnet. The whole HTS motor is cooled by liquid nitrogen to 77K, and acts as a permanent magnet synchronous motor with the power rate of 15.7 kW.

  6. Experiments with a dc motor

    International Nuclear Information System (INIS)

    Kraftmakher, Yaakov

    2010-01-01

    Experiments with an electric motor provide good opportunity to demonstrate some basic laws of electricity and magnetism. The aim of the experiments with a low-power dc motor is to show how the motor approaches its steady rotation and how its torque, mechanical power and efficiency depend on the rotation velocity. The tight relationship between the mechanical and electrical parameters of the motor is clearly seen. The measurements are carried out with the ScienceWorkshop data-acquisition system and the DataStudio software from PASCO scientific. The experiments are well related to university courses of electricity and magnetism and can be used in undergraduate laboratories or for lecture demonstrations.

  7. Harmonic modeling of induction motors

    Energy Technology Data Exchange (ETDEWEB)

    Pedra, J.; Sainz, L.; Corcoles, F. [Department of Electrical Engineering, ETSEIB-UPC, Av. Diagonal 647, 08028 Barcelona (Spain)

    2006-07-15

    The paper proposes an induction motor model for the study of harmonic load flow in balanced and unbalanced conditions. The parameters of this model are obtained from motor manufacturer data and the positive- and negative-sequence equivalent circuits of the single- and double-cage models. An approximate harmonic model based on motor manufacturer data only is also proposed. In addition, the paper includes manufacturer data and the calculated parameters of 36 induction motors of different rated powers. This database is used to analyze the proposed models. (author)

  8. Motor carrier evaluation program

    International Nuclear Information System (INIS)

    Portsmouth, James

    1992-01-01

    The U.S. Department of Energy-Headquarters (DOE-HQ), Transportation Management Program (TMP) has the overall responsibility to provide a well-managed transportation program for the safe, efficient, and economical transportation of DOE-owned materials. The DOE-TMP has established an excellent safety record in the transportation of hazardous materials including radioactive materials and radioactive wastes. This safety record can be maintained only through continued diligence and sustained effort on the part of the DOE-TMP, its field offices, and the contractors' organizations. Key elements in the DOE'S effective hazardous and radioactive materials shipping program are (1) integrity of packages, (2) strict adherence to regulations and procedures, (3) trained personnel, (4) complete management support, and (5) use of the best commercial carriers. The DOE Motor Carrier Evaluation Program was developed to better define the criteria and methodology needed to identify motor carriers for use in the transportation of Highway Route Controlled Quantities (HRCQ), Truck Load (TL) quantities of radioactive materials, hazardous materials and waste. (author)

  9. Zenn Motor Company

    Energy Technology Data Exchange (ETDEWEB)

    Clifford, I. [Zenn Motor Company, Toronto, ON (Canada)

    2010-07-01

    Zenn Motor company is a leader in the electric vehicle space and builds and markets a low speed vehicle known as the zero emission, no noise (ZENN). This presentation provided background information on the Zenn Motor Company as well as on EEStor, a company that develops four-wheeled vehicles and that is seeking partners to fund the development for a modified barium titanate-based ultracapacitor. In 2004, ZENN entered into a technology agreement with EEStor that secured certain exclusive and non-exclusive rights to purchase and deploy EEStor's EESU technology as part of its ZENNergy solutions in several markets, including exclusive rights for new four-passenger vehicles with a curb weight of up to 1,400 kilograms; exclusive rights for the neighbourhood electric vehicles (NEV) and golf carts market; exclusive rights for utility vehicles; and exclusive rights for the aftermarket conversion to ZENNergy of any four-wheeled vehicles. The presentation also addressed ZENNergy and the art of integrating high energy drive solutions. Lessons learned and EEStor's technology attributes were discussed. A hypothetical case study was also offered. The presentation concluded with a discussion of EEStor technology status and opportunity horizons. It was concluded that a better battery is needed to enable the mass adoption of electric vehicles. tabs.

  10. Motor-operated valve (MOV) actuator motor and gearbox testing

    International Nuclear Information System (INIS)

    DeWall, K.; Watkins, J.C.; Bramwell, D.

    1997-07-01

    Researchers at the Idaho National Engineering and Environmental Laboratory tested the performance of electric motors and actuator gearboxes typical of the equipment installed on motor-operated valves used in nuclear power plants. Using a test stand that simulates valve closure loads against flow and pressure, the authors tested five electric motors (four ac and one dc) and three gearboxes at conditions a motor might experience in a power plant, including such off-normal conditions as operation at high temperature and reduced voltage. They also monitored the efficiency of the actuator gearbox. All five motors operated at or above their rated starting torque during tests at normal voltages and temperatures. For all five motors, actual torque losses due to voltage degradation were greater than the losses calculated by methods typically used for predicting motor torque at degraded voltage conditions. For the dc motor the actual torque losses due to elevated operating temperatures were greater than the losses calculated by the typical predictive method. The actual efficiencies of the actuator gearboxes were generally lower than the running efficiencies published by the manufacturer and were generally nearer the published pull-out efficiencies. Operation of the gearbox at elevated temperature did not affect the operating efficiency

  11. Torque control for electric motors

    Science.gov (United States)

    Bernard, C. A.

    1980-01-01

    Method for adjusting electric-motor torque output to accomodate various loads utilizes phase-lock loop to control relay connected to starting circuit. As load is imposed, motor slows down, and phase lock is lost. Phase-lock signal triggers relay to power starting coil and generate additional torque. Once phase lock is recoverd, relay restores starting circuit to its normal operating mode.

  12. Open-Ended Electric Motor

    Science.gov (United States)

    Gould, Mauri

    1975-01-01

    Presents complete instructions for assembling an electric motor which does not require large amounts of power to operate and which is inexpensive as well as reliable. Several open-ended experiments with the motor are included as well as information for obtaining a kit of parts and instructions. (BR)

  13. Genetic heterogeneity of motor neuropathies.

    Science.gov (United States)

    Bansagi, Boglarka; Griffin, Helen; Whittaker, Roger G; Antoniadi, Thalia; Evangelista, Teresinha; Miller, James; Greenslade, Mark; Forester, Natalie; Duff, Jennifer; Bradshaw, Anna; Kleinle, Stephanie; Boczonadi, Veronika; Steele, Hannah; Ramesh, Venkateswaran; Franko, Edit; Pyle, Angela; Lochmüller, Hanns; Chinnery, Patrick F; Horvath, Rita

    2017-03-28

    To study the prevalence, molecular cause, and clinical presentation of hereditary motor neuropathies in a large cohort of patients from the North of England. Detailed neurologic and electrophysiologic assessments and next-generation panel testing or whole exome sequencing were performed in 105 patients with clinical symptoms of distal hereditary motor neuropathy (dHMN, 64 patients), axonal motor neuropathy (motor Charcot-Marie-Tooth disease [CMT2], 16 patients), or complex neurologic disease predominantly affecting the motor nerves (hereditary motor neuropathy plus, 25 patients). The prevalence of dHMN is 2.14 affected individuals per 100,000 inhabitants (95% confidence interval 1.62-2.66) in the North of England. Causative mutations were identified in 26 out of 73 index patients (35.6%). The diagnostic rate in the dHMN subgroup was 32.5%, which is higher than previously reported (20%). We detected a significant defect of neuromuscular transmission in 7 cases and identified potentially causative mutations in 4 patients with multifocal demyelinating motor neuropathy. Many of the genes were shared between dHMN and motor CMT2, indicating identical disease mechanisms; therefore, we suggest changing the classification and including dHMN also as a subcategory of Charcot-Marie-Tooth disease. Abnormal neuromuscular transmission in some genetic forms provides a treatable target to develop therapies. Copyright © 2017 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.

  14. Energy-saving motor; Energiesparmotor

    Energy Technology Data Exchange (ETDEWEB)

    Lindegger, M.

    2002-07-01

    This report for the Swiss Federal Office of Energy (SFOE) describes the development and testing of an advanced electrical motor using a permanent-magnet rotor. The aims of the project - to study the technical feasibility and market potential of the Eco-Motor - are discussed and the three phases of the project described. These include the calculation and realisation of a 250-watt prototype operating at 230 V, the measurement of the motor's characteristics as well as those of a comparable asynchronous motor on the test bed at the University of Applied Science in Lucerne, Switzerland, and a market study to establish if the Eco-Motor and its controller can compete against normal asynchronous motors. Also, the results of an analysis of the energy-savings potential is made, should such Eco-Motors be used. Detailed results of the three phases of the project are presented and the prospects of producing such motors in Switzerland for home use as well as for export are examined.

  15. Advances in esophageal motor disorders

    NARCIS (Netherlands)

    Smout, André Jpm

    2008-01-01

    PURPOSE OF REVIEW: Esophageal motor disorders, often leading to dysphagia and chest pain, continue to pose diagnostic and therapeutic problems. In the past 12 months important new information regarding esophageal motor disorders was published. This information will be reviewed in this paper. RECENT

  16. Advances in esophageal motor disorders

    NARCIS (Netherlands)

    Smout, Andre J. P. M.

    Purpose of review Esophageal motor disorders, often leading to dysphagia and chest pain, continue to pose diagnostic and therapeutic problems. In the past 12 months important new information regarding esophageal motor disorders was published. This information will be reviewed in this paper. Recent

  17. 77 FR 26607 - Energy Conservation Program: Test Procedures for Electric Motors and Small Electric Motors

    Science.gov (United States)

    2012-05-04

    ... Conservation Program: Test Procedures for Electric Motors and Small Electric Motors; Final Rules #0;#0;Federal... Procedures for Electric Motors and Small Electric Motors AGENCY: Office of Energy Efficiency and Renewable... electric motors and small electric motors. That supplemental proposal, along with an earlier proposal from...

  18. Piezoelectric wave motor

    Science.gov (United States)

    Yerganian, Simon Scott

    2001-07-17

    A piezoelectric motor having a stator in which piezoelectric elements are contained in slots formed in the stator transverse to the desired wave motion. When an electric field is imposed on the elements, deformation of the elements imposes a force perpendicular to the sides of the slot, deforming the stator. Appropriate frequency and phase shifting of the electric field will produce a wave in the stator and motion in a rotor. In a preferred aspect, the piezoelectric elements are configured so that deformation of the elements in direction of an imposed electric field, generally referred to as the d.sub.33 direction, is utilized to produce wave motion in the stator. In a further aspect, the elements are compressed into the slots so as to minimize tensile stresses on the elements in use.

  19. Reciprocating linear motor

    Science.gov (United States)

    Goldowsky, Michael P. (Inventor)

    1987-01-01

    A reciprocating linear motor is formed with a pair of ring-shaped permanent magnets having opposite radial polarizations, held axially apart by a nonmagnetic yoke, which serves as an axially displaceable armature assembly. A pair of annularly wound coils having axial lengths which differ from the axial lengths of the permanent magnets are serially coupled together in mutual opposition and positioned with an outer cylindrical core in axial symmetry about the armature assembly. One embodiment includes a second pair of annularly wound coils serially coupled together in mutual opposition and an inner cylindrical core positioned in axial symmetry inside the armature radially opposite to the first pair of coils. Application of a potential difference across a serial connection of the two pairs of coils creates a current flow perpendicular to the magnetic field created by the armature magnets, thereby causing limited linear displacement of the magnets relative to the coils.

  20. Economical motor transport operations

    Energy Technology Data Exchange (ETDEWEB)

    Cooke, P

    1979-09-01

    Motor transport is one area in a company where energy conservation is a function primarily of operative education and motivation rather than mechanical or technical control and monitoring. Unless the driver wants to save energy by proper operation of the vehicle, there is nothing the company can do to force him, whatever equipment it fits to the vehicles, or incentives it offers. This article gives an overview of the use of energy in road transport and examines a number of actions that can be taken to conserve energy. It discusses the question of the cost-effectiveness of transport energy conservation in the light of the complex issues involved. The problems and opportunities of implementing energy-saving programs are examined. (MCW)

  1. Stabilizing motor fuels

    Energy Technology Data Exchange (ETDEWEB)

    1935-07-12

    Motor fuel is stabilized by adding less than 2% of a tar fraction from peat, coal, torbanite or shale, said fraction containing sufficient constituents boiling between 200 and 325/sup 0/C, to inhibit gum formation. Low-temperature coal-tar fractions are specified. The preferred boiling ranges are from 225 or 250/sup 0/ to 275/sup 0/C. In examples, the quantity added was 0.01%. The fuel may be a cracked distillate of gasoline boiling-point range or containing gasoline, and may contain relatively large proportions of di- and tri-olefines. The material added to the fuel may be (1) the tar fraction itself; (2) its alkali-soluble constituents; (3) its acid-soluble constituents; (4) a mixture of (2) and (3); (5) a blend of (2), (3) or (4) with a normal tar fraction; (6) the residue after extraction with alkali; (7) the residue after extraction with acid and alkali.

  2. Sucker rod motor

    Energy Technology Data Exchange (ETDEWEB)

    Radzalov, N N; Radzhabov, N A

    1983-01-01

    The motor consists of rollers mounted on the wellmouth and connected by a flexible rink. Reciprocating mechanism is in the form of a horizontal non-mobile single-side operation cylinder, inside which a plunger and rod are mounted. The working housing of the hydrocylinder is connected to a gas-hydr aulic batter, and when running is connected via plunger to the high pressure source; running in reverse it is connected with a safety valve and automatic control unit. The unit is equipped with a reducer and a mechanical transformer consisting of screw and nut, and which is shutoff with a single-side lining. The plunger rod consists of an auger-like unit. The high pressure source is provided by the injection line of the sucker rod that has been equipped with a reverse valve.

  3. Motor-operated gearbox efficiency

    Energy Technology Data Exchange (ETDEWEB)

    DeWall, K.G.; Watkins, J.C.; Bramwell, D. [Idaho National Engineering Lab., Idaho Falls, ID (United States); Weidenhamer, G.H.

    1996-12-01

    Researchers at the Idaho National Engineering Laboratory recently conducted tests investigating the operating efficiency of the power train (gearbox) in motor-operators typically used in nuclear power plants to power motor-operated valves. Actual efficiency ratios were determined from in-line measurements of electric motor torque (input to the operator gearbox) and valve stem torque (output from the gearbox) while the operators were subjected to gradually increasing loads until the electric motor stalled. The testing included parametric studies under reduced voltage and elevated temperature conditions. As part of the analysis of the results, the authors compared efficiency values determined from testing to the values published by the operator manufacturer and typically used by the industry in calculations for estimating motor-operator capabilities. The operators they tested under load ran at efficiencies lower than the running efficiency (typically 50%) published by the operator manufacturer.

  4. Motor-operated gearbox efficiency

    International Nuclear Information System (INIS)

    DeWall, K.G.; Watkins, J.C.; Bramwell, D.; Weidenhamer, G.H.

    1996-01-01

    Researchers at the Idaho National Engineering Laboratory recently conducted tests investigating the operating efficiency of the power train (gearbox) in motor-operators typically used in nuclear power plants to power motor-operated valves. Actual efficiency ratios were determined from in-line measurements of electric motor torque (input to the operator gearbox) and valve stem torque (output from the gearbox) while the operators were subjected to gradually increasing loads until the electric motor stalled. The testing included parametric studies under reduced voltage and elevated temperature conditions. As part of the analysis of the results, the authors compared efficiency values determined from testing to the values published by the operator manufacturer and typically used by the industry in calculations for estimating motor-operator capabilities. The operators they tested under load ran at efficiencies lower than the running efficiency (typically 50%) published by the operator manufacturer

  5. Motor-operator gearbox efficiency

    International Nuclear Information System (INIS)

    DeWall, K.G.; Watkins, J.C.; Bramwell, D.

    1996-01-01

    Researchers at the Idaho National Engineering Laboratory recently conducted tests investigating the operating efficiency of the power train (gearbox) in motor-operators typically used in nuclear power plants to power motor-operated valves. Actual efficiency ratios were determined from in-line measurements of electric motor torque (input to the operator gearbox) and valve stem torque (output from the gearbox) while the operators were subjected to gradually increasing loads until the electric motor stalled. The testing included parametric studies under reduced voltage and elevated temperature conditions. As part of the analysis of the results, we compared efficiency values determined from testing to the values published by the operator manufacturer and typically used by the industry in calculations for estimating motor-operator capabilities. The operators we tested under load ran at efficiencies lower than the running efficiency (typically 50%) published by the operator manufacturer

  6. Electric motors for use in radiation environments

    International Nuclear Information System (INIS)

    Aslam, T.U.D.; Mahmood, S.B.

    1981-01-01

    Requirements of electric motors for a nuclear plant and the effect of nuclear radiations on different parts of the motors are discussed. Feasibility of using locally-fabricated motors is also considered. (author)

  7. VERY SLOW SPEED AXIAL MOTION RELUCTANCE MOTOR

    African Journals Online (AJOL)

    Dr Obe

    1984-09-01

    Sep 1, 1984 ... VERY SLOW SPEED AXIAL MOTION RELUCTANCE MOTOR by. L. A. Agu ... order as that of the screw-thread motor can be obtained. LIST OF .... The n stator have equal non- magnetic spacers .... induction motor. An.

  8. Actions to promote energy efficient electric motors. Motors study group

    Energy Technology Data Exchange (ETDEWEB)

    Almeida, A.T. de [Coimbra Univ. (PT). Inst. of Systems and Robotics (ISR)

    1996-10-01

    Motor electricity consumption is influenced by many factors including: motor efficiency, motor speed controls, power supply quality, harmonics, systems oversizing, distribution network, mechanical transmission system, maintenance practices, load management and cycling, and the efficiency of the end-use device (e.g. fan, pump, etc.). Due to their importance, an overview of these factors is presented in this report. This study also describes the electricity use in the industrial and tertiary sectors and the electricity consumption associated with the different types of electric motors systems in the Member States of the European Union, as well as estimated future evolution until 2010. The studies for individual countries were carried out by the different partners of the motors study group at a previous stage. The study has found that there is a lack of accurate information about the motor electricity consumption, installed motor capacity and the motor market in almost all the European Union countries and only some general statistical sources are available. There is little field data, which is mainly available in Denmark, France, Italy and the Netherlands. Due to this lack of primary information, some common assumptions were made, based on the experience of the members of the study group. This lack of end-use characterisation data shows the need for improvement from the point of view of current knowledge. It is therefore recommended that further research is undertaken to arrive at more accurate figures. These could be the basis for a better understanding for motor use in practice and - as a consequence - for a more precise appraisal of potentials and barriers to energy efficiency. (orig.)

  9. Submersible canned motor mixer pump

    International Nuclear Information System (INIS)

    Guardiani, R.F.; Pollick, R.D.

    1997-01-01

    A mixer pump is described used in a waste tank for mobilizing high-level radioactive liquid waste having a column assembly containing power cables, a motor housing with electric motor means which includes a stator can of a stator assembly and a rotor can of a rotor assembly, and an impeller assembly with an impeller connected to a shaft of the rotor assembly. The column assembly locates the motor housing with the electric motor means adjacent to the impeller which creates an hydraulic head, and which forces the liquid waste into the motor housing to cool the electric motor means and to lubricate radial and thrust bearing assemblies. Hard-on-hard bearing surfaces of the bearing assemblies and a ring assembly between the impeller and electric motor means act to grind down large particles in the liquid waste flow. These larger particles are received in slots in the static bearing members of the radial bearing assemblies. Only solid waste particles smaller than the clearances in the system can pass there through, thereby resisting damage to and the interruption of the operation of the mixer pump. 10 figs

  10. Pathogenesis of motor neuron disease

    Institute of Scientific and Technical Information of China (English)

    Xuefei Wang

    2006-01-01

    OBJECTIVE: To summarize and analyze the factors and theories related to the attack of motor neuron disease, and comprehensively investigate the pathogenesis of motor neuron disease.DATA SOURCES: A search of Pubmed database was undertaken to identify articles about motor neuron disease published in English from January 1994 to June 2006 by using the keywords of "neurodegenerative diseases". Other literatures were collected by retrieving specific journals and articles.STUDY SELECTION: The data were checked primarily, articles related to the pathogenesis of motor neuron disease were involved, and those obviously irrelated to the articles were excluded.DATA EXTRACTION: Totally 54 articles were collected, 30 of them were involved, and the other 24 were excluded.DATA SYNTHESIS: The pathogenesis of motor neuron disease has multiple factors, and the present related theories included free radical oxidation, excitotoxicity, genetic and immune factors, lack of neurotrophic factor,injury of neurofilament, etc. The studies mainly come from transgenic animal models, cell culture in vitro and patients with familial motor neuron disease, but there are still many restrictions and disadvantages.CONCLUSION: It is necessary to try to find whether there is internal association among different mechanisms,comprehensively investigate the pathogenesis of motor neuron diseases, in order to provide reliable evidence for the clinical treatment.

  11. Hereditary motor neuropathies and motor neuron diseases: which is which.

    Science.gov (United States)

    Hanemann, Clemens O; Ludolph, Albert C

    2002-12-01

    When Charcot first defined amyotrophic lateral sclerosis (ALS) he used the clinical and neuropathological pattern of vulnerability as a guideline. Similarly other motor neuron diseases such as the spinal muscular atrophies (SMA) and the motor neuropathies (MN) were grouped following clinical criteria. However, ever since the etiology of these diseases has started to be disclosed by genetics, we have learnt that the limits of the syndromes are not as well defined as our forefathers thought. A mutation leading to ALS can also be associated with the clinical picture of spinal muscular atrophy; even more unexpected is the overlap of the so-called motor neuropathies with the clinical syndrome of slowly progressive ALS or that primary lateral sclerosis (PLS) can be caused by the same gene as that responsible for some cases of ALS. In this review we summarise recent work showing that there is a considerable overlap between CMT, MN, SMA, ALS and PLS. Insights into these phenotypes should lead to study of the variants of motor neuron disease and possibly to a reclassification. This comprehensive review should help to improve understanding of the pathogenesis of motor neuron degeneration and finally may aid the research for urgently needed new treatment strategies, perhaps with validity for the entire group of motor neuron diseases.

  12. The change in perceived motor competence and motor task values during elementary school : Gender and motor performance differences

    NARCIS (Netherlands)

    Noordstar, J.J.; van der Net, J.; Jak, S.; Helders, P.J.M.; Jongmans, M.J.

    2016-01-01

    Participation in motor activities is essential for social interaction and life satisfaction in children. Self-perceptions and task values have a central position in why children do or do not participate in (motor) activities. Investigating developmental changes in motor self-perceptions and motor

  13. 'Motor control center obsolescence'

    International Nuclear Information System (INIS)

    Irish, C.S.

    2003-01-01

    A significant and growing problem within the global nuclear industry is the aging of motor control center (MCC) components. MCC's have a very important role in the safety and critical to generation requirements of a nuclear power plant. Although many OEM's MCC's such as ITE/Telemechanique, GE, Westinghouse, Cutler Hammer, Klockner Moeller, etc. have been used throughout the global nuclear industry, they all have one common aspect obsolescence. Obsolescence of various components within the MCC's such as molded case circuit breakers, starters, relays, heaters, contactors, etc. are impacting the reliability of the MCC to serve its intended function. The paper will discuss the options which the nuclear industry is faced with to increase the reliability of the MCC's while maintaining design control, qualification and meeting budget constraints. The options as listed below shall be discussed in detail with examples to enhance the readers understanding of the situation: 1) Component by component replacement: The hurdles associated with trying to find equivalent components to replace the obsolete components while still worki (mechanically and electrically) in the original cubicle will be presented. 2) Complete MCC cubicle with new internal components replacement: The process of supplying a replacement cubicle, with all new internal components and new door to replace the original cubicle will be discussed. The presentation will conclude with a comparison of the advantages and dis-advantages of the two methods to bring the MCC to an as new condition with the overall goal of increasing reliability. (author)

  14. Aberrant supplementary motor complex and limbic activity during motor preparation in motor conversion disorder.

    Science.gov (United States)

    Voon, Valerie; Brezing, Christina; Gallea, Cecile; Hallett, Mark

    2011-11-01

    Conversion disorder (CD) is characterized by unexplained neurological symptoms presumed related to psychological issues. The main hypotheses to explain conversion paralysis, characterized by a lack of movement, include impairments in either motor intention or disruption of motor execution, and further, that hyperactive self-monitoring, limbic processing or top-down regulation from higher order frontal regions may interfere with motor execution. We have recently shown that CD with positive abnormal or excessive motor symptoms was associated with greater amygdala activity to arousing stimuli along with greater functional connectivity between the amygdala and supplementary motor area. Here we studied patients with such symptoms focusing on motor initiation. Subjects performed either an internally or externally generated 2-button action selection task in a functional MRI study. Eleven CD patients without major depression and 11 age- and gender-matched normal volunteers were assessed. During both internally and externally generated movement, conversion disorder patients relative to normal volunteers had lower left supplementary motor area (SMA) (implicated in motor initiation) and higher right amygdala, left anterior insula, and bilateral posterior cingulate activity (implicated in assigning emotional salience). These findings were confirmed in a subgroup analysis of patients with tremor symptoms. During internally versus externally generated action in CD patients, the left SMA had lower functional connectivity with bilateral dorsolateral prefrontal cortices. We propose a theory in which previously mapped conversion motor representations may in an arousing context hijack the voluntary action selection system, which is both hypoactive and functionally disconnected from prefrontal top-down regulation. Copyright © 2011 Movement Disorder Society.

  15. Aberrant supplementary motor complex and limbic activity during motor preparation in motor conversion disorder

    Science.gov (United States)

    Voon, V; Brezing, C; Gallea, C; Hallett, M

    2014-01-01

    Background Conversion disorder is characterized by unexplained neurological symptoms presumed related to psychological issues. The main hypotheses to explain conversion paralysis, characterized by a lack of movement, include impairments in either motor intention or disruption of motor execution, and further, that hyperactive self-monitoring, limbic processing or top-down regulation from higher order frontal regions may interfere with motor execution. We have recently shown that conversion disorder with positive abnormal or excessive motor symptoms was associated with greater amygdala activity to arousing stimuli along with greater functional connectivity between the amgydala and supplementary motor area. Here we studied patients with such symptoms focusing on motor initiation. Methods Subjects performed either an internally or externally generated two-button action selection task in a functional MRI study. Results Eleven conversion disorder patients without major depression and 11 age- and gender-matched normal volunteers were assessed. During both internally and externally generated movement, conversion disorder patients relative to normal volunteers had lower left supplementary motor area (SMA) (implicated in motor initiation) and higher right amygdala, left anterior insula and bilateral posterior cingulate activity (implicated in assigning emotional salience). These findings were confirmed in a subgroup analysis of patients with tremor symptoms. During internally versus externally generated action in CD patients, the left SMA had lower functional connectivity with bilateral dorsolateral prefrontal cortices. Conclusion We propose a theory in which previously mapped conversion motor representations may in an arousing context hijack the voluntary action selection system which is both hypoactive and functionally disconnected from prefrontal top-down regulation. PMID:21935985

  16. Honda Motor Company's CVCC Engine

    Science.gov (United States)

    1980-01-01

    From 1968 to 1972, Honda Motor Co. of Japan designed, tested, and massproduced a stratified charge engine, the CVCC, which was lower in carbon monoxide, hydrocarbon, and nitrogen oxide emissions and higher in fuel economy than contemporary convention...

  17. Fault diagnosis of induction motors

    CERN Document Server

    Faiz, Jawad; Joksimović, Gojko

    2017-01-01

    This book is a comprehensive, structural approach to fault diagnosis strategy. The different fault types, signal processing techniques, and loss characterisation are addressed in the book. This is essential reading for work with induction motors for transportation and energy.

  18. 78 FR 66801 - Motor Carrier Safety Advisory Committee; Charter Renewal

    Science.gov (United States)

    2013-11-06

    ... DEPARTMENT OF TRANSPORTATION Federal Motor Carrier Safety Administration [Docket No. FMCSA-2006-26367] Motor Carrier Safety Advisory Committee; Charter Renewal AGENCY: Federal Motor Carrier Safety... and recommendations on motor carrier safety programs and motor carrier safety regulations through a...

  19. Electrical stimulation and motor recovery.

    Science.gov (United States)

    Young, Wise

    2015-01-01

    In recent years, several investigators have successfully regenerated axons in animal spinal cords without locomotor recovery. One explanation is that the animals were not trained to use the regenerated connections. Intensive locomotor training improves walking recovery after spinal cord injury (SCI) in people, and >90% of people with incomplete SCI recover walking with training. Although the optimal timing, duration, intensity, and type of locomotor training are still controversial, many investigators have reported beneficial effects of training on locomotor function. The mechanisms by which training improves recovery are not clear, but an attractive theory is available. In 1949, Donald Hebb proposed a famous rule that has been paraphrased as "neurons that fire together, wire together." This rule provided a theoretical basis for a widely accepted theory that homosynaptic and heterosynaptic activity facilitate synaptic formation and consolidation. In addition, the lumbar spinal cord has a locomotor center, called the central pattern generator (CPG), which can be activated nonspecifically with electrical stimulation or neurotransmitters to produce walking. The CPG is an obvious target to reconnect after SCI. Stimulating motor cortex, spinal cord, or peripheral nerves can modulate lumbar spinal cord excitability. Motor cortex stimulation causes long-term changes in spinal reflexes and synapses, increases sprouting of the corticospinal tract, and restores skilled forelimb function in rats. Long used to treat chronic pain, motor cortex stimuli modify lumbar spinal network excitability and improve lower extremity motor scores in humans. Similarly, epidural spinal cord stimulation has long been used to treat pain and spasticity. Subthreshold epidural stimulation reduces the threshold for locomotor activity. In 2011, Harkema et al. reported lumbosacral epidural stimulation restores motor control in chronic motor complete patients. Peripheral nerve or functional electrical

  20. Mathematical modeling of molecular motors

    OpenAIRE

    Keller, Peter

    2013-01-01

    Amongst the many complex processes taking place in living cells, transport of cargoes across the cytosceleton is fundamental to cell viability and activity. To move cargoes between the different cell parts, cells employ Molecular Motors. The motors operate by transporting cargoes along the so-called cellular micro-tubules, namely rope-like structures that connect, for instance, the cell-nucleus and outer membrane. We introduce a new Markov Chain, the killed Quasi-Random-Walk, for such transpo...

  1. Segmented rail linear induction motor

    Science.gov (United States)

    Cowan, Jr., Maynard; Marder, Barry M.

    1996-01-01

    A segmented rail linear induction motor has a segmented rail consisting of a plurality of nonferrous electrically conductive segments aligned along a guideway. The motor further includes a carriage including at least one pair of opposed coils fastened to the carriage for moving the carriage. A power source applies an electric current to the coils to induce currents in the conductive surfaces to repel the coils from adjacent edges of the conductive surfaces.

  2. Motor performance of preschool children

    OpenAIRE

    Karina Słonka; Manuela Dyas; Tadeusz Słonka; Tomasz Szurmik

    2017-01-01

    Introduction: Pre‑school age is a period of intensive development when children shape their posture, habits and motor memory. Movement is child's physiological need.  Motive activity supports not only physical development, but also psychical, intellectual and social.   Aim: The aim of the study is to assess motor ability in preschool children from the city of Opole and District Dobrzeń Wielki. Materials and methods: The research involved 228 children, aged 5 and 6. The method used in...

  3. Paliperidone-associated motor tics.

    Science.gov (United States)

    Hsieh, Ming-Han; Chiu, Nan-Ying

    2014-01-01

    Paliperidone-associated motor tics. Case report. We report a 30-year-old man with schizophrenia who developed motor tics (eye blinking) after treatment of paliperidone up to 15 mg daily. Tic-like symptoms, from simple eye blinking to complex Tourette-like syndrome, may occur during paliperidone treatment, especially with high dose. Crown Copyright © 2014. Published by Elsevier Inc. All rights reserved.

  4. High-speed AC motors

    Energy Technology Data Exchange (ETDEWEB)

    Jokinen, T.; Arkkio, A. [Helsinki University of Technology Laboratory of Electromechanics, Otaniemi (Finland)

    1997-12-31

    The paper deals with various types of highspeed electric motors, and their limiting powers. Standard machines with laminated rotors can be utilised if the speed is moderate. The solid rotor construction makes it possible to reach higher power and speed levels than those of laminated rotors. The development work on high-speed motors done at Helsinki University of Technology is presented, too. (orig.) 12 refs.

  5. Humoral and cellular immune responses in BALB/c and C57BL/6 mice immunized with cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive antigens, in acute experimental Trypanosoma cruzi infection.

    Science.gov (United States)

    Pereira, Valéria R A; Lorena, Virginia M B; Nakazawa, Mineo; Luna, Carlos F; Silva, Edimilson D; Ferreira, Antonio G P; Krieger, Marco Aurélio; Goldenberg, Samuel; Soares, Milena B P; Coutinho, Eridan M; Correa-Oliveira, Rodrigo; Gomes, Yara M

    2005-06-01

    In previous studies, cytoplasmic repetitive antigen (CRA) and flagellar repetitive antigen (FRA) proteins induced specific humoral and cellular immune responses in susceptible and resistant mice in the absence of Trypanosoma cruzi infection with a significant induction of the Interferon-gamma (IFN-gamma) production in those animals. In this follow-up paper, the immunostimulatory and protective effects of these proteins were evaluated by immunizing with CRA or FRA antigens, BALB/c and C57BL/6 mice and challenging with a T. cruzi (Y strain). Both proteins induced humoral response with high levels of IgG isotypes as well as cellular immunity with high levels of IFN-gamma when compared to controls. However, the lymphocyte proliferative response was minimal. The survival rate at 30 days post-infection was significant in CRA (60%) or FRA (50%)--immunized BALB/c mice and CRA (83.3%)--immunized C57BL/6 mice. Taken as a whole these findings indicate that CRA and FRA are immunogenic and potentially important for protective immunity.

  6. Transcriptional Analysis of the MrpJ Network: Modulation of Diverse Virulence-Associated Genes and Direct Regulation of mrp Fimbrial and flhDC Flagellar Operons in Proteus mirabilis

    Science.gov (United States)

    Bode, Nadine J.; Debnath, Irina; Kuan, Lisa; Schulfer, Anjelique; Ty, Maureen

    2015-01-01

    The enteric bacterium Proteus mirabilis is associated with a significant number of catheter-associated urinary tract infections (UTIs). Strict regulation of the antagonistic processes of adhesion and motility, mediated by fimbriae and flagella, respectively, is essential for disease progression. Previously, the transcriptional regulator MrpJ, which is encoded by the mrp fimbrial operon, has been shown to repress both swimming and swarming motility. Here we show that MrpJ affects an array of cellular processes beyond adherence and motility. Microarray analysis found that expression of mrpJ mimicking levels observed during UTIs leads to differential expression of 217 genes related to, among other functions, bacterial virulence, type VI secretion, and metabolism. We probed the molecular mechanism of transcriptional regulation by MrpJ using transcriptional reporters and chromatin immunoprecipitation (ChIP). Binding of MrpJ to two virulence-associated target gene promoters, the promoters of the flagellar master regulator flhDC and mrp itself, appears to be affected by the condensation state of the native chromosome, although both targets share a direct MrpJ binding site proximal to the transcriptional start. Furthermore, an mrpJ deletion mutant colonized the bladders of mice at significantly lower levels in a transurethral model of infection. Additionally, we observed that mrpJ is widely conserved in a collection of recent clinical isolates. Altogether, these findings support a role of MrpJ as a global regulator of P. mirabilis virulence. PMID:25847961

  7. Electric motor for laser-mechanical drilling

    Science.gov (United States)

    Grubb, Daryl L.; Faircloth, Brian O.; Zediker, Mark S.

    2015-07-07

    A high power laser drilling system utilizing an electric motor laser bottom hole assembly. A high power laser beam travels within the electric motor for advancing a borehole. High power laser drilling system includes a down hole electrical motor having a hollow rotor for conveying a high power laser beam through the electrical motor.

  8. Electric motor for laser-mechanical drilling

    Science.gov (United States)

    Grubb, Daryl L.; Faircloth, Brian O.; Zediker, Mark S.

    2017-10-10

    A high power laser drilling system utilizing an electric motor laser bottom hole assembly. A high power laser beam travels within the electric motor for performing a laser operation. A system includes a down hole electrical motor having a hollow rotor for conveying a high power laser beam having a wavelength less than 1060 nm through the electrical motor.

  9. A proton-driven, intense, subcritical, fission neutron source for radioisotope production

    Energy Technology Data Exchange (ETDEWEB)

    Jongen, Y. [Chemin du Cyclotron, Louvain-la-Neuve (Belgium)

    1995-10-01

    {sup 99m}Tc, the most frequently used radioisotope in nuclear medicine, is distributed as {sup 99}Mo=>{sup 99m}Tc generators. {sup 99}Mo is a fission product of {sup 235}U. To replace the aging nuclear reactors used today for this production, the author proposes to use a spallation neutron source, with neutron multiplication by fission. A 150 MeV, H{sup {minus}} cyclotron can produce a 225 kW proton beam with 50% total system energy efficiency. The proton beam would hit a molten lead target, surrounded by a water moderator and a graphite reflector, producing around 0.96 primary neutron per proton. The primary spallation neutrons, moderated, would strike secondary targets containing a subcritical amount of {sup 235}U. The assembly would show a k{sub eff} of 0.8, yielding a fivefold neutron multiplication. The thermal neutron flux at the targets location would be 2 {times} 10{sup 14} n/cm{sup 2}.s, resulting in a fission power of 500 to 750 kW. One such system could supply the world demand in {sup 99}Mo, as well as other radioisotopes. Preliminary indications show that the cost would be lower than the cost of a commercial 10 MW isotope production reactor. The cost of operation, of disposal of radiowaste and of decommissioning should be significantly lower as well. Finally, the non-critical nature of the system would make it more acceptable for the public than a nuclear reactor and should simplify the licensing process.

  10. Accelerator Studies on a possible Experiment on Proton-Driven Plasma Wakefields at CERN

    CERN Document Server

    Assmann, R W; Fartoukh, S; Geschonke, G; Goddard, B; Hessler, C; Hillenbrand, S; Meddahi, M; Roesler, S; Zimmermann, F; Caldwell, A; Muggli, P; Xia, G

    2011-01-01

    There has been a proposal by Caldwell et al to use proton beams as drivers for high energy linear colliders. An experimental test with CERN’s proton beams is being studied. Such a test requires a transfer line for transporting the beam to the experiment, a focusing section for beam delivery into the plasma, the plasma cell and a downstream diagnostics and dump section. The work done at CERN towards the conceptual layout and design of such a test area is presented. A possible development of such a test area into a CERN test facility for high-gradient acceleration experiments is discussed.

  11. Control of non-conventional synchronous motors

    CERN Document Server

    Louis, Jean-Paul

    2013-01-01

    Classical synchronous motors are the most effective device to drive industrial production systems and robots with precision and rapidity. However, numerous applications require efficient controls in non-conventional situations. Firstly, this is the case with synchronous motors supplied by thyristor line-commutated inverters, or with synchronous motors with faults on one or several phases. Secondly, many drive systems use non-conventional motors such as polyphase (more than three phases) synchronous motors, synchronous motors with double excitation, permanent magnet linear synchronous motors,

  12. Movement Sonification: Audiovisual benefits on motor learning

    Directory of Open Access Journals (Sweden)

    Weber Andreas

    2011-12-01

    Full Text Available Processes of motor control and learning in sports as well as in motor rehabilitation are based on perceptual functions and emergent motor representations. Here a new method of movement sonification is described which is designed to tune in more comprehensively the auditory system into motor perception to enhance motor learning. Usually silent features of the cyclic movement pattern "indoor rowing" are sonified in real time to make them additionally available to the auditory system when executing the movement. Via real time sonification movement perception can be enhanced in terms of temporal precision and multi-channel integration. But beside the contribution of a single perceptual channel to motor perception and motor representation also mechanisms of multisensory integration can be addressed, if movement sonification is configured adequately: Multimodal motor representations consisting of at least visual, auditory and proprioceptive components - can be shaped subtly resulting in more precise motor control and enhanced motor learning.

  13. 41 CFR 102-34.85 - What motor vehicles require motor vehicle identification?

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What motor vehicles require motor vehicle identification? 102-34.85 Section 102-34.85 Public Contracts and Property Management... 34-MOTOR VEHICLE MANAGEMENT Identifying and Registering Motor Vehicles Motor Vehicle Identification...

  14. Applications of superconductors to electric motors

    International Nuclear Information System (INIS)

    McConnell, B.W.

    1988-01-01

    This paper reviews previous experience in applying superconductors to electric motors and examines the difficulties encountered. While motors and generators have a common basis, several significant differences exist. The application of high temperature superconductors to the major electric motor types is discussed and expected difficulties are presented. The limitations imposed by various motor designs are reflected in a statement of the desired material properties for high temperature superconductor electric motor applications

  15. Space shuttle booster separation motor design

    Science.gov (United States)

    Smith, G. W.; Chase, C. A.

    1976-01-01

    The separation characteristics of the space shuttle solid rocket boosters (SRBs) are introduced along with the system level requirements for the booster separation motors (BSMs). These system requirements are then translated into specific motor requirements that control the design of the BSM. Each motor component is discussed including its geometry, material selection, and fabrication process. Also discussed is the propellant selection, grain design, and performance capabilities of the motor. The upcoming test program to develop and qualify the motor is outlined.

  16. Motor demand-dependent activation of ipsilateral motor cortex.

    Science.gov (United States)

    Buetefisch, Cathrin M; Revill, Kate Pirog; Shuster, Linda; Hines, Benjamin; Parsons, Michael

    2014-08-15

    The role of ipsilateral primary motor cortex (M1) in hand motor control during complex task performance remains controversial. Bilateral M1 activation is inconsistently observed in functional (f)MRI studies of unilateral hand performance. Two factors limit the interpretation of these data. As the motor tasks differ qualitatively in these studies, it is conceivable that M1 contributions differ with the demand on skillfulness. Second, most studies lack the verification of a strictly unilateral execution of the motor task during the acquisition of imaging data. Here, we use fMRI to determine whether ipsilateral M1 activity depends on the demand for precision in a pointing task where precision varied quantitatively while movement trajectories remained equal. Thirteen healthy participants used an MRI-compatible joystick to point to targets of four different sizes in a block design. A clustered acquisition technique allowed simultaneous fMRI/EMG data collection and confirmed that movements were strictly unilateral. Accuracy of performance increased with target size. Overall, the pointing task revealed activation in contralateral and ipsilateral M1, extending into contralateral somatosensory and parietal areas. Target size-dependent activation differences were found in ipsilateral M1 extending into the temporal/parietal junction, where activation increased with increasing demand on accuracy. The results suggest that ipsilateral M1 is active during the execution of a unilateral motor task and that its activity is modulated by the demand on precision. Copyright © 2014 the American Physiological Society.

  17. Changes of motor-cortical oscillations associated with motor learning.

    Science.gov (United States)

    Pollok, B; Latz, D; Krause, V; Butz, M; Schnitzler, A

    2014-09-05

    Motor learning results from practice but also between practice sessions. After skill acquisition early consolidation results in less interference with other motor tasks and even improved performance of the newly learned skill. A specific significance of the primary motor cortex (M1) for early consolidation has been suggested. Since synchronized oscillatory activity is assumed to facilitate neuronal plasticity, we here investigate alterations of motor-cortical oscillations by means of event-related desynchronization (ERD) at alpha (8-12 Hz) and beta (13-30 Hz) frequencies in healthy humans. Neuromagnetic activity was recorded using a 306-channel whole-head magnetoencephalography (MEG) system. ERD was investigated in 15 subjects during training on a serial reaction time task and 10 min after initial training. The data were compared with performance during a randomly varying sequence serving as control condition. The data reveal a stepwise decline of alpha-band ERD associated with faster reaction times replicating previous findings. The amount of beta-band suppression was significantly correlated with reduction of reaction times. While changes of alpha power have been related to lower cognitive control after initial skill acquisition, the present data suggest that the amount of beta suppression represents a neurophysiological marker of early cortical reorganization associated with motor learning. Copyright © 2014 IBRO. Published by Elsevier Ltd. All rights reserved.

  18. Submersible canned motor transfer pump

    International Nuclear Information System (INIS)

    Guardiani, R.F.; Pollick, R.D.; Nyilas, C.P.; Denmeade, T.J.

    1997-01-01

    A transfer pump is described which is used in a waste tank for transferring high-level radioactive liquid waste from a waste tank and having a column assembly, a canned electric motor means, and an impeller assembly with an upper impeller and a lower impeller connected to a shaft of a rotor assembly. The column assembly locates a motor housing with the electric motor means adjacent to the impeller assembly which creates an hydraulic head, and which forces the liquid waste, into the motor housing to cool the electric motor means and to cool and/or lubricate the radial and thrust bearing assemblies. Hard-on-hard bearing surfaces of the bearing assemblies and a ring assembly between the upper impeller and electric motor means grind large particles in the liquid waste flow. Slots in the static bearing member of the radial bearing assemblies further grind down the solid waste particles so that only particles smaller than the clearances in the system can pass there through, thereby resisting damage to and the interruption of the operation of the transfer pump. The column assembly is modular so that sections can be easily assembled, disassembled and/or removed. A second embodiment employs a stator jacket which provides an alternate means for cooling the electric motor means and lubricating and/or cooling the bearing assemblies, and a third embodiment employs a variable level suction device which allows liquid waste to be drawn into the transfer pump from varying and discrete levels in the waste tank. 17 figs

  19. 77 FR 65765 - Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; General...

    Science.gov (United States)

    2012-10-30

    ... the vehicle. The antenna module translates the radio frequency signal received from the key into a... DEPARTMENT OF TRANSPORTATION National Highway Traffic Safety Administration Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; General Motors Corporation AGENCY...

  20. 77 FR 25534 - Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; General...

    Science.gov (United States)

    2012-04-30

    ... response back to the vehicle. The antenna module translates the radio frequency signal received from the... DEPARTMENT OF TRANSPORTATION National Highway Traffic Safety Administration Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; General Motors Corporation AGENCY...

  1. 75 FR 22317 - Federal Motor Vehicle Safety Standards; Small Business Impacts of Motor Vehicle Safety

    Science.gov (United States)

    2010-04-28

    ... 1300 [Docket No. NHTSA-2010-0054] Federal Motor Vehicle Safety Standards; Small Business Impacts of Motor Vehicle Safety AGENCY: National Highway Traffic Safety Administration (NHTSA), Department of..., multipurpose passenger vehicles, trucks, buses, trailers, incomplete vehicles, motorcycles, and motor vehicle...

  2. Comparison of Linear Induction Motor Theories for the LIMRV and TLRV Motors

    Science.gov (United States)

    1978-01-01

    The Oberretl, Yamamura, and Mosebach theories of the linear induction motor are described and also applied to predict performance characteristics of the TLRV & LIMRV linear induction motors. The effect of finite motor width and length on performance ...

  3. 46 CFR 169.684 - Overcurrent protection for motors and motor branch circuits.

    Science.gov (United States)

    2010-10-01

    ...) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Machinery and Electrical Electrical Installations Operating at... motor that is responsive to motor current or to both motor current and temperature may be used. (b) The...

  4. On line protection systems for induction motors

    International Nuclear Information System (INIS)

    Colak, I.; Celik, H.; Sefa, I.; Demirbas, S.

    2005-01-01

    Protection of induction motors is very important since they are widely used in industry for many applications due to their high robustness, reliability, low cost and maintenance, high efficiency and long service life. So, protecting these motors is crucial for operations. This paper presents a combined protection approach for induction motors. To achieve this, the electrical values of the induction motor were measured with sensitivity ±1% through a data acquisition card and processed with software developed in Visual C++. An on line protection system for induction motors was achieved easily and effectively. The experimental results have shown that the induction motor was protected against the possible problems faced during the operation. The software developed for this protection provides flexible and reliable media for operators and their motors. It is expected that the motor protection achieved in this study might be faster than the classical techniques and also may be applied to larger motors easily after small modifications of the software

  5. Mirror neurons and motor intentionality.

    Science.gov (United States)

    Rizzolatti, Giacomo; Sinigaglia, Corrado

    2007-01-01

    Our social life rests to a large extent on our ability to understand the intentions of others. What are the bases of this ability? A very influential view is that we understand the intentions of others because we are able to represent them as having mental states. Without this meta-representational (mind-reading) ability their behavior would be meaningless to us. Over the past few years this view has been challenged by neurophysiological findings and, in particular, by the discovery of mirror neurons. The functional properties of these neurons indicate that intentional understanding is based primarily on a mechanism that directly matches the sensory representation of the observed actions with one's own motor representation of those same actions. These findings reveal how deeply motor and intentional components of action are intertwined, suggesting that both can be fully comprehended only starting from a motor approach to intentionality.

  6. Induction Motors by Electric Measurements

    Directory of Open Access Journals (Sweden)

    Andrzej M. Trzynadlowski

    1999-01-01

    Full Text Available The paper gives an overview of the issues and means of detection of mechanical abnormalities in induction motors by electric measurements. If undetected and untreated, the worn or damaged bearings, rotor imbalance and eccentricity, broken bars of the rotor cage, and torsional and lateral vibration lead to roughly a half of all failures of induction motor drives. The detection of abnormalities is based on the fact that they cause periodic disturbance of motor variables, such as the speed, torque, current, and magnetic flux. Thus, spectral analysis of those or related quantities may yield a warning about an incipient failure of the drive system. Although the traditional non-invasive diagnostics has mostly been based on the signature analysis of the stator current, other media can also be employed. In particular, the partial instantaneous input power is shown, theoretically and experimentally, to offer distinct advantages under noisy operating conditions. Use of torque and flux estimates is also discussed.

  7. Linear Synchronous Motor Repeatability Tests

    International Nuclear Information System (INIS)

    Ward, C.R.

    2002-01-01

    A cart system using linear synchronous motors was being considered for the Plutonium Immobilization Plant (PIP). One of the applications in the PIP was the movement of a stack of furnace trays, filled with the waste form (pucks) from a stacking/unstacking station to several bottom loaded furnaces. A system was ordered to perform this function in the PIP Ceramic Prototype Test Facility (CPTF). This system was installed and started up in SRTC prior to being installed in the CPTF. The PIP was suspended and then canceled after the linear synchronous motor system was started up. This system was used to determine repeatability of a linear synchronous motor cart system for the Modern Pit Facility

  8. Conceptual design of stepper motor replacing servo motor for control rod controller

    International Nuclear Information System (INIS)

    Mohd Dzul Aiman Aslan; Mohd Idris Taib; Izhar Abu Hussin; Mohd Khairulezwan Abdul Manan; Mohd Sabri Minhat

    2010-01-01

    In PUSPATI TRIGA Reactor, current control rod controller are using servo motor to control the movement. Control rod is a very important safety element and measure in every nuclear reactor. So, precision is very important in measurement of security in the nuclear reactor. In this case, there are a few disadvantages when using the servo motor is measurement of the motor is not precise. One solution to overcome this is by shifting servo motor with stepper motor. A stepper motor (or step motor) is a brush less, synchronous electric motor that can divide a full rotation into a large number of steps. (author)

  9. Superconducting DC homopolar motors for ship propulsion

    Energy Technology Data Exchange (ETDEWEB)

    Heiberger, M.; Reed, M.R.; Creedon, W.P.; O' Hea, B.J. [General Atomic (United States)

    2000-07-01

    Superconducting DC homopolar motors have undergone recent advances in technology, warranting serious consideration of their use for ship propulsion. Homopolar motor propulsion is now practical because of two key technology developments: cryogen-free superconducting refrigeration and high performance motor fiber brushes. These compact motors are ideal for podded applications, where reduced drag and fuel consumption are predicted. In addition, the simple DC motor controller is more efficient and reliable compared with AC motor controllers. Military ships also benefit from increased stealth implicit in homopolar DC excitation, which also allows the option for direct hull or pod mounting. (authors)

  10. Homopolar motor with dual rotors

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, J.S.

    1998-12-01

    A homopolar motor has a field rotor mounted on a frame for rotation in a first rotational direction and for producing an electromagnetic field, and an armature rotor mounted for rotation on said frame within said electromagnetic field and in a second rotational direction counter to said first rotational direction of said field rotor. The two rotors are coupled through a 1:1 gearing mechanism, so as to travel at the same speed but in opposite directions. This doubles the output voltage and output power, as compared to a motor in which only the armature is rotated. Several embodiments are disclosed. 7 figs.

  11. Homopolar motor with dual rotors

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, John S. (Oak Ridge, TN)

    1998-01-01

    A homopolar motor (10) has a field rotor (15) mounted on a frame (11) for rotation in a first rotational direction and for producing an electromagnetic field, and an armature rotor (17) mounted for rotation on said frame (11) within said electromagnetic field and in a second rotational direction counter to said first rotational direction of said field rotor (15). The two rotors (15, 17) are coupled through a 1:1 gearing mechanism (19), so as to travel at the same speed but in opposite directions. This doubles the output voltage and output power, as compared to a motor in which only the armature is rotated. Several embodiments are disclosed.

  12. The Advanced Solid Rocket Motor

    Science.gov (United States)

    Mitchell, Royce E.

    1992-01-01

    The Advanced Solid Rocket Motor will utilize improved design features and automated manufacturing methods to produce an inherently safer propulsive system for the Space Shuttle and future launch systems. This second-generation motor will also provide an additional 12,000 pounds of payload to orbit, enhancing the utility and efficiency of the Shuttle system. The new plant will feature strip-wound, asbestos-free insulation; propellant continuous mixing and casting; and extensive robotic systems. Following a series of static tests at the Stennis Space Center, MS flights are targeted to begin in early 1997.

  13. Interference in ballistic motor learning - is motor interference really sensory?

    DEFF Research Database (Denmark)

    Lundbye-Jensen, Jesper; Petersen, Tue Hvass; Rothwell, John C

    Skill gained after a short period of practice in one motor task can be abolished if a second task is learned shortly afterwards. We hypothesised that interference requires the same circuits to be engaged in the two tasks and provoke competing processes of synaptic plasticity. To test this, subjects...

  14. To what extent does motor imagery resemble motor preparation?

    NARCIS (Netherlands)

    van der Lubbe, Rob; Sobierajewicz, Jagna; Jongsma, Marijtje; Przekoracka-Krawczyk, Anna

    2017-01-01

    Motor imagery may be defined as the generation of an image of the acting self that lacks the final execution of a movement. This image is thought to be a simulation of the intended action from a first-person perspective. Recent studies with a Go/NoGo version of the discrete sequence production

  15. Motor learning as a criterion for evaluating coordination motor abilities

    Directory of Open Access Journals (Sweden)

    Boraczynski Tomasz

    2011-10-01

    Full Text Available The aim of the study was to evaluate the ability of motor learning based on objective, metric criteria, in terms of pedagogical process aimed at improving the accuracy of hits a golf ball to the target. A group of 77 students of physical education participated in the study. Within 8 months there were performed 11 measurement sessions. In each session, subjects performed 10 hits a golf ball to the target from a distance of 9 m. Accuracy of hits was recorded. Effect of motor learning has been demonstrated in the progress of 10 consecutive hits a golf ball to the target in each session (operational control; in the dynamics of performance improvement between sessions (current control; as well as in the total result of eight-month experiment (stage control. There were developed norms for quantitative and qualitative assessment of accuracy of hits a golf ball to the target. Developed quantitative and qualitative criteria for assessing the speed of motor learning in various conditions of the educational process creates the possibility of organization the operational, current and stage control of the level of human coordination motor abilities, as required by leading process.

  16. Coupling with concentric contact around motor shaft for line start synchronous motor

    Science.gov (United States)

    Melfi, Michael J.; Burdeshaw, Galen E.

    2017-10-03

    A method comprises providing a line-start synchronous motor. The motor has a stator, a rotor core disposed within the stator, and a motor shaft. In accordance with a step of the method, a coupling for coupling a load to the motor is provided. The coupling has a motor shaft attachment portion configured to provide substantially concentric contact around the shaft at the end of the motor shaft. The coupling has a load attachment portion configured to operatively connect to a load. In accordance with a step of the method, a load is coupled to the motor with the coupling, and driven from start to at least near synchronous speed during steady state operation of the motor with a load coupled thereto. The motor shaft attachment portion may comprise a bushing assembly with matching and opposed tapered surfaces that cooperate to secure the motor shaft attachment portion around the motor shaft.

  17. Motor cortex is required for learning but not for executing a motor skill.

    Science.gov (United States)

    Kawai, Risa; Markman, Timothy; Poddar, Rajesh; Ko, Raymond; Fantana, Antoniu L; Dhawale, Ashesh K; Kampff, Adam R; Ölveczky, Bence P

    2015-05-06

    Motor cortex is widely believed to underlie the acquisition and execution of motor skills, but its contributions to these processes are not fully understood. One reason is that studies on motor skills often conflate motor cortex's established role in dexterous control with roles in learning and producing task-specific motor sequences. To dissociate these aspects, we developed a motor task for rats that trains spatiotemporally precise movement patterns without requirements for dexterity. Remarkably, motor cortex lesions had no discernible effect on the acquired skills, which were expressed in their distinct pre-lesion forms on the very first day of post-lesion training. Motor cortex lesions prior to training, however, rendered rats unable to acquire the stereotyped motor sequences required for the task. These results suggest a remarkable capacity of subcortical motor circuits to execute learned skills and a previously unappreciated role for motor cortex in "tutoring" these circuits during learning. Copyright © 2015 Elsevier Inc. All rights reserved.

  18. Motor cortex is required for learning but not executing a motor skill

    Science.gov (United States)

    Kawai, Risa; Markman, Timothy; Poddar, Rajesh; Ko, Raymond; Fantana, Antoniu; Dhawale, Ashesh; Kampff, Adam R.; Ölveczky, Bence P.

    2018-01-01

    Motor cortex is widely believed to underlie the acquisition and execution of motor skills, yet its contributions to these processes are not fully understood. One reason is that studies on motor skills often conflate motor cortex’s established role in dexterous control with roles in learning and producing task-specific motor sequences. To dissociate these aspects, we developed a motor task for rats that trains spatiotemporally precise movement patterns without requirements for dexterity. Remarkably, motor cortex lesions had no discernible effect on the acquired skills, which were expressed in their distinct pre-lesion forms on the very first day of post-lesion training. Motor cortex lesions prior to training, however, rendered rats unable to acquire the stereotyped motor sequences required for the task. These results suggest a remarkable capacity of subcortical motor circuits to execute learned skills and a previously unappreciated role for motor cortex in ‘tutoring’ these circuits during learning. PMID:25892304

  19. Aversive stimuli exacerbate defensive motor behaviour in motor conversion disorder.

    Science.gov (United States)

    Blakemore, Rebekah L; Sinanaj, Indrit; Galli, Silvio; Aybek, Selma; Vuilleumier, Patrik

    2016-12-01

    Conversion disorder or functional neurological symptom disorder (FND) can affect the voluntary motor system, without an organic cause. Functional symptoms are thought to be generated unconsciously, arising from underlying psychological stressors. However, attempts to demonstrate a direct relationship between the limbic system and disrupted motor function in FND are lacking. We tested whether negative affect would exacerbate alterations of motor control and corresponding brain activations in individuals with FND. Ten patients and ten healthy controls produced an isometric precision-grip contraction at 10% of maximum force while either viewing visual feedback of their force output, or unpleasant or pleasant emotional images (without feedback). Force magnitude was continuously recorded together with change in brain activity using fMRI. For controls, force output decayed from the target level while viewing pleasant and unpleasant images. Patients however, maintained force at the target level without decay while viewing unpleasant images, indicating a pronounced effect of negative affect on force output in FND. This emotional modulation of force control was associated with different brain activation patterns between groups. Contrasting the unpleasant with the pleasant condition, controls showed increased activity in the inferior frontal cortex and pre-supplementary motor area, whereas patients had greater activity in the cerebellum (vermis), posterior cingulate cortex, and hippocampus. Engagement of a cerebellar-limbic network in patients is consistent with heightened processing of emotional salience, and supports the role of the cerebellum in freezing responses in the presence of aversive events. These data highlight a possible neural circuit through which psychological stressors elicit defensive behaviour and modulate motor function in FND. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Transcriptional analysis of the MrpJ network: modulation of diverse virulence-associated genes and direct regulation of mrp fimbrial and flhDC flagellar operons in Proteus mirabilis.

    Science.gov (United States)

    Bode, Nadine J; Debnath, Irina; Kuan, Lisa; Schulfer, Anjelique; Ty, Maureen; Pearson, Melanie M

    2015-06-01

    The enteric bacterium Proteus mirabilis is associated with a significant number of catheter-associated urinary tract infections (UTIs). Strict regulation of the antagonistic processes of adhesion and motility, mediated by fimbriae and flagella, respectively, is essential for disease progression. Previously, the transcriptional regulator MrpJ, which is encoded by the mrp fimbrial operon, has been shown to repress both swimming and swarming motility. Here we show that MrpJ affects an array of cellular processes beyond adherence and motility. Microarray analysis found that expression of mrpJ mimicking levels observed during UTIs leads to differential expression of 217 genes related to, among other functions, bacterial virulence, type VI secretion, and metabolism. We probed the molecular mechanism of transcriptional regulation by MrpJ using transcriptional reporters and chromatin immunoprecipitation (ChIP). Binding of MrpJ to two virulence-associated target gene promoters, the promoters of the flagellar master regulator flhDC and mrp itself, appears to be affected by the condensation state of the native chromosome, although both targets share a direct MrpJ binding site proximal to the transcriptional start. Furthermore, an mrpJ deletion mutant colonized the bladders of mice at significantly lower levels in a transurethral model of infection. Additionally, we observed that mrpJ is widely conserved in a collection of recent clinical isolates. Altogether, these findings support a role of MrpJ as a global regulator of P. mirabilis virulence. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  1. ELECTRIC MOTORS FOR FARM USE.

    Science.gov (United States)

    Illinois Univ., Urbana. Coll. of Agriculture.

    BETWEEN 2 AND 8 HOURS ARE REQUIRED FOR USE OF THIS TEXTUAL OR REFERENCE MATERIAL ON ELECTRIC MOTORS. IT WAS DEVELOPED BY AN AGRICULTURAL EDUCATION-AGRICULTURAL ENGINEERING SPECIALIST ON THE BASIS OF CONFERENCES WITH SUBJECT MATTER SPECIALISTS, TEACHER EDUCATORS, SUPERVISORS, AND TEACHERS. THE OBJECTIVES AND SUBJECT MATTER CENTER AROUND THE…

  2. Imaging the ocular motor nerves.

    NARCIS (Netherlands)

    Ferreira, T.; Verbist, B.M.; Buchem, M. van; Osch, T. van; Webb, A.

    2010-01-01

    The ocular motor nerves (OMNs) comprise the oculomotor, trochlear and the abducens nerves. According to their course, they are divided into four or five anatomic segments: intra-axial, cisternal, cavernous and intra-orbital and, for the abducens nerve, an additional interdural segment. Magnetic

  3. Linear Motor With Air Slide

    Science.gov (United States)

    Johnson, Bruce G.; Gerver, Michael J.; Hawkey, Timothy J.; Fenn, Ralph C.

    1993-01-01

    Improved linear actuator comprises air slide and linear electric motor. Unit exhibits low friction, low backlash, and more nearly even acceleration. Used in machinery in which positions, velocities, and accelerations must be carefully controlled and/or vibrations must be suppressed.

  4. Technology and Motor Ability Development

    Science.gov (United States)

    Wang, Lin; Lang, Yong; Luo, Zhongmin

    2014-01-01

    As a new member joining the technology family, active video games have been developed to promote physical exercise. This working-in-progress paper shares an ongoing project on examining the basic motor abilities that are enhanced through participating in commercially available active video games. [For the full proceedings see ED557181.

  5. Motor Skill Learning in Children.

    Science.gov (United States)

    Gabbard, Carl P.

    The purpose of this article is to briefly describe schema theory and indicate its relevance to early childhood development, with specific reference to children's acquisition of motor skills. Schema theory proposes an explanation of how individuals learn and perform a seemingly endless variety of movements. According to Schmidt (1975), goal…

  6. Shape memory alloy based motor

    Indian Academy of Sciences (India)

    1989a,b) that SMA spring or strip generates force as a non-linear function of its deformed length. ... magnetic property, smooth, jerk free operation and insensitivity to space radiations namely, electrons, protons ..... will be 1 Amp DC. The motor ...

  7. Treatment of functional motor disorders

    NARCIS (Netherlands)

    Gelauff, Jeannette M.; Dreissen, Yasmine E. M.; Tijssen, Marina A. J.; Stone, Jon

    OPINION STATEMENT: For the treatment of functional motor disorder, we recommend a three-stage approach. Firstly, patients must be assessed and given an unambiguous diagnosis, with an explanation that helps them understand that they have a genuine disorder, with the potential for reversibility. A key

  8. Motor Action and Emotional Memory

    Science.gov (United States)

    Casasanto, Daniel; Dijkstra, Katinka

    2010-01-01

    Can simple motor actions affect how efficiently people retrieve emotional memories, and influence what they choose to remember? In Experiment 1, participants were prompted to retell autobiographical memories with either positive or negative valence, while moving marbles either upward or downward. They retrieved memories faster when the direction…

  9. Magnetic Signature of Brushless Electric Motors

    National Research Council Canada - National Science Library

    Clarke, David

    2006-01-01

    Brushless electric motors are used in a number of underwater vehicles. When these underwater vehicles are used for mine clearance operations the magnetic signature of the brushless motors is important...

  10. Improving commercial motor vehicle safety in Oregon.

    Science.gov (United States)

    2010-08-01

    This study addressed the primary functions of the Oregon Department of Transportations (ODOTs) Motor Carrier Safety Assistance Program (MCSAP), which is administered by the Motor Carrier Transportation Division (MCTD). The study first documente...

  11. Energy Optimal Control of Induction Motor Drives

    DEFF Research Database (Denmark)

    Abrahamsen, Flemming

    This thesis deals with energy optimal control of small and medium-size variable speed induction motor drives for especially Heating, Ventilation and Air-Condition (HVAC) applications. Optimized efficiency is achieved by adapting the magnetization level in the motor to the load, and the basic...... demonstrated that energy optimal control will sometimes improve and sometimes deteriorate the stability. Comparison of small and medium-size induction motor drives with permanent magnet motor drives indicated why, and in which applications, PM motors are especially good. Calculations of economical aspects...... improvement by energy optimal control for any standard induction motor drive between 2.2 kW and 90 kW. A simple method to evaluate the robustness against load disturbances was developed and used to compare the robustness of different motor types and sizes. Calculation of the oscillatory behavior of a motor...

  12. Experimental thermodynamics of single molecular motor.

    Science.gov (United States)

    Toyabe, Shoichi; Muneyuki, Eiro

    2013-01-01

    Molecular motor is a nano-sized chemical engine that converts chemical free energy to mechanical motions. Hence, the energetics is as important as kinetics in order to understand its operation principle. We review experiments to evaluate the thermodynamic properties of a rotational F1-ATPase motor (F1-motor) at a single-molecule level. We show that the F1-motor achieves 100% thermo dynamic efficiency at the stalled state. Furthermore, the motor reduces the internal irreversible heat inside the motor to almost zero and achieves a highly-efficient free energy transduction close to 100% during rotations far from quasistatic process. We discuss the mechanism of how the F1-motor achieves such a high efficiency, which highlights the remarkable property of the nano-sized engine F1-motor.

  13. Motor Vehicle Crash Injuries PSA (:60)

    Centers for Disease Control (CDC) Podcasts

    This 60 second Public Service Announcement is based on the October 2014 CDC Vital Signs report. Motor vehicle crashes are costly and preventable. Learn what can be done to help prevent motor vehicle injuries.

  14. Optimization analysis of propulsion motor control efficiency

    Directory of Open Access Journals (Sweden)

    CAI Qingnan

    2017-12-01

    Full Text Available [Objectives] This paper aims to strengthen the control effect of propulsion motors and decrease the energy used during actual control procedures.[Methods] Based on the traditional propulsion motor equivalence circuit, we increase the iron loss current component, introduce the definition of power matching ratio, calculate the highest efficiency of a motor at a given speed and discuss the flux corresponding to the power matching ratio with the highest efficiency. In the original motor vector efficiency optimization control module, an efficiency optimization control module is added so as to achieve motor efficiency optimization and energy conservation.[Results] MATLAB/Simulink simulation data shows that the efficiency optimization control method is suitable for most conditions. The operation efficiency of the improved motor model is significantly higher than that of the original motor model, and its dynamic performance is good.[Conclusions] Our motor efficiency optimization control method can be applied in engineering to achieve energy conservation.

  15. How to make spinal motor neurons.

    Science.gov (United States)

    Davis-Dusenbery, Brandi N; Williams, Luis A; Klim, Joseph R; Eggan, Kevin

    2014-02-01

    All muscle movements, including breathing, walking, and fine motor skills rely on the function of the spinal motor neuron to transmit signals from the brain to individual muscle groups. Loss of spinal motor neuron function underlies several neurological disorders for which treatment has been hampered by the inability to obtain sufficient quantities of primary motor neurons to perform mechanistic studies or drug screens. Progress towards overcoming this challenge has been achieved through the synthesis of developmental biology paradigms and advances in stem cell and reprogramming technology, which allow the production of motor neurons in vitro. In this Primer, we discuss how the logic of spinal motor neuron development has been applied to allow generation of motor neurons either from pluripotent stem cells by directed differentiation and transcriptional programming, or from somatic cells by direct lineage conversion. Finally, we discuss methods to evaluate the molecular and functional properties of motor neurons generated through each of these techniques.

  16. Ultrasonic Linear Motor with Two Independent Vibrations

    Science.gov (United States)

    Muneishi, Takeshi; Tomikawa, Yoshiro

    2004-09-01

    We propose a new structure of an ultrasonic linear motor in order to solve the problems of high-power ultrasonic linear motors that drive the XY-stage for electron beam equipment and to expand the application fields of the motor. We pay special attention to the following three points: (1) the vibration in two directions of the ultrasonic linear motor should not influence mutually each other, (2) the vibration in two directions should be divided into the stage traveling direction and the pressing direction of the ultrasonic linear motor, and (3) the rigidity of the stage traveling direction of the ultrasonic linear motor should be increased. As a result, the supporting method of ultrasonic linear motors is simplified. The efficiency of the motor is improved and temperature rise is reduced. The stage position drift is also improved.

  17. Ventajas de los motores de flujo axial

    Directory of Open Access Journals (Sweden)

    Alberto M Basanta Otero

    2011-03-01

    Full Text Available Es importante conocer sobre una familia de motores que a diferencia de los convencionales o tradicionales no presentanun flujo rotatorio radial, denominados motores de flujo axial. Dichos motores presentan altos valores de par motriz abajas velocidades, una alta eficiencia y alta densidad de potencia. Este trabajo constituye un breve análisis dealgunos motores de la referencia bibliográfica.  Is important to know about a family of motors that at difference whit the traditional, don't have a rotator radial flux,called, axial flux motors. These motors have high torque for low speed, high efficiency and high power density. Thiswork is a brief analysis of several motors of the bibliographic references.

  18. The micro-step motor controller

    International Nuclear Information System (INIS)

    Hong, Kwang Pyo; Lee, Chang Hee; Moon, Myung Kook; Choi, Bung Hun; Choi, Young Hyun; Cheon, Jong Gu

    2004-11-01

    The developed micro-step motor controller can handle 4 axes stepping motor drivers simultaneously and provide high power bipolar driving mechanism with constant current mode. It can be easily controlled by manual key functions and the motor driving status is displayed by the front panel VFD. Due to the development of several kinds of communication and driving protocol, PC can operate even several micro-step motor controllers at once by multi-drop connection

  19. High-Tc superconducting electric motors

    International Nuclear Information System (INIS)

    Schiferl, R.; Stein, J.

    1992-01-01

    In this paper, the advantages and limitations of using superconductors in motors are discussed. A synchronous motor with a high temperature superconducting field winding for pump and fan drive applications is described and some of its unique design features are identified. A 10,000 horsepower superconducting motor design is presented. The critical field and current density requirements for high temperature superconducting wire in motors is discussed. Finally, recent progress in superconducting wire performance is presented

  20. Frequency modulation drive for a piezoelectric motor

    Science.gov (United States)

    Mittas, Anthony

    2001-01-01

    A piezoelectric motor has peak performance at a specific frequency f.sub.1 that may vary over a range of frequencies. A drive system is disclosed for operating such a motor at peak performance without feedback. The drive system consists of the motor and an ac source connected to power the motor, the ac source repeatedly generating a frequency over a range from f.sub.1 -.DELTA.x to f.sub.1 +.DELTA.y.

  1. Some simple demonstration experiments involving homopolar motors

    OpenAIRE

    Stewart, Seán M.

    2007-01-01

    The ready availability of very strong permanent magnets in the form of rare-earth magnetic alloys such as neodymium-iron-boron has lead to renewed interest in one of the oldest types of electric motors - the homopolar motor. The ease with which a demonstration homopolar motor can now be built and operated when neodymium magnets are used is quite remarkable. In this paper some simple homopolar motors employing neodymium magnets suitable for demonstrational purposes are described and discussed....

  2. Some simple demonstration experiments involving homopolar motors

    OpenAIRE

    Stewart,Seán M.

    2007-01-01

    The ready availability of very strong permanent magnets in the form of rare-earth magnetic alloys such as neodymium-iron-boron has lead to renewed interest in one of the oldest types of electric motors - the homopolar motor. The ease with which a demonstration homopolar motor can now be built and operated when neodymium magnets are used is quite remarkable. In this paper some simple homopolar motors employing neodymium magnets suitable for demonstrational purposes are described and discussed.

  3. Physical mechanisms of biological molecular motors

    Energy Technology Data Exchange (ETDEWEB)

    Miller, John H. Jr. [Department of Physics and Texas Center for Superconductivity, University of Houston, 4800 Calhoun Road, Ste. 617 SR1 Houston, TX 77204-5005 (United States)], E-mail: jhmiller@uh.edu; Vajrala, Vijayanand; Infante, Hans L. [Department of Physics and Texas Center for Superconductivity, University of Houston, 4800 Calhoun Road, Ste. 617 SR1 Houston, TX 77204-5005 (United States); Claycomb, James R. [Department of Physics and Texas Center for Superconductivity, University of Houston, 4800 Calhoun Road, Ste. 617 SR1 Houston, TX 77204-5005 (United States); Department of Mathematics and Physics, Houston Baptist University, 7502 Fondren Road, Houston, TX 77074-3298 (United States); Palanisami, Akilan; Fang Jie; Mercier, George T. [Department of Physics and Texas Center for Superconductivity, University of Houston, 4800 Calhoun Road, Ste. 617 SR1 Houston, TX 77204-5005 (United States)

    2009-03-01

    Biological motors generally fall into two categories: (1) those that convert chemical into mechanical energy via hydrolysis of a nucleoside triphosphate, usually adenosine triphosphate, regarded as life's chemical currency of energy and (2) membrane bound motors driven directly by an ion gradient and/or membrane potential. Here we argue that electrostatic interactions play a vital role for both types of motors and, therefore, the tools of physics can greatly contribute to understanding biological motors.

  4. Physical mechanisms of biological molecular motors

    International Nuclear Information System (INIS)

    Miller, John H. Jr.; Vajrala, Vijayanand; Infante, Hans L.; Claycomb, James R.; Palanisami, Akilan; Fang Jie; Mercier, George T.

    2009-01-01

    Biological motors generally fall into two categories: (1) those that convert chemical into mechanical energy via hydrolysis of a nucleoside triphosphate, usually adenosine triphosphate, regarded as life's chemical currency of energy and (2) membrane bound motors driven directly by an ion gradient and/or membrane potential. Here we argue that electrostatic interactions play a vital role for both types of motors and, therefore, the tools of physics can greatly contribute to understanding biological motors

  5. FUZZY LOGIC CONTROL OF ELECTRIC MOTORS AND MOTOR DRIVES: FEASIBILITY STUDY

    Science.gov (United States)

    The report gives results of a study (part 1) of fuzzy logic motor control (FLMC). The study included: 1) reviews of existing applications of fuzzy logic, of motor operation, and of motor control; 2) a description of motor control schemes that can utilize FLMC; 3) selection of a m...

  6. Het motorrijwiel : handboek voor motor- en scooterrijders, motor- en scootermonteurs en -technici

    NARCIS (Netherlands)

    Seyffardt, A.L.W.

    1961-01-01

    INHOUD: 1. De werking van de motor ; 2. De hoofddelen van de motor ; 3. De kleppen en de klepbeweging ; 4. Balancering, de meercilinder motor ; 5. Bijzonderheden over de werking ; 6. De carburateur ; 7. De smering van de motor ; 8. De tweetaktmotor ; 9. De elektrische installatie ; 10. De

  7. The influence of motor imagery on the learning of a fine hand motor skill

    NARCIS (Netherlands)

    Sobierajewicz, Jagna; Przekoracka-Krawczyk, Anna; Jaśkowski, Wojciech; Verwey, Willem B.; van der Lubbe, Rob

    2017-01-01

    Motor imagery has been argued to affect the acquisition of motor skills. The present study examined the specificity of motor imagery on the learning of a fine hand motor skill by employing a modified discrete sequence production task: the Go/NoGo DSP task. After an informative cue, a response

  8. 76 FR 12792 - Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; General...

    Science.gov (United States)

    2011-03-08

    ... From the Federal Motor Vehicle Motor Theft Prevention Standard; General Motors Corporation AGENCY..., Exemption from the Theft Prevention Standard. This petition is granted because the agency has determined... in reducing and deterring motor vehicle theft as compliance with the parts-marking requirements of...

  9. 77 FR 22383 - Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; TESLA

    Science.gov (United States)

    2012-04-13

    ... From the Federal Motor Vehicle Motor Theft Prevention Standard; TESLA AGENCY: National Highway Traffic... exemption. SUMMARY: This document grants in full the petition of Tesla Motors Inc's. (Tesla) for an... 49 CFR Part 541, Federal Motor Vehicle Theft Prevention Standard. Tesla requested confidential...

  10. Neuronal mechanisms of motor learning and motor memory consolidation in healthy old adults

    NARCIS (Netherlands)

    Berghuis, K. M. M.; Veldman, M. P.; Solnik, S.; Koch, G.; Zijdewind, I.; Hortobagyi, T.

    It is controversial whether or not old adults are capable of learning new motor skills and consolidate the performance gains into motor memory in the offline period. The underlying neuronal mechanisms are equally unclear. We determined the magnitude of motor learning and motor memory consolidation

  11. Assessment of Preschoolers' Gross Motor Proficiency: Revisiting Bruininks-Oseretsky Test of Motor Proficiency

    Science.gov (United States)

    Lam, Hazel Mei Yung

    2011-01-01

    Literature reveals that there are very few validated motor proficiency tests for young children. According to Gallahue and Ozmun, the Bruininks-Oseretsky Test of Motor Proficiency is a valid test. However, manipulative skills, which are classified as gross motor skills by most motor development specialists, are only tested in the Upper Limb…

  12. Motor Programming in Apraxia of Speech

    Science.gov (United States)

    Maas, Edwin; Robin, Donald A.; Wright, David L.; Ballard, Kirrie J.

    2008-01-01

    Apraxia of Speech (AOS) is an impairment of motor programming. However, the exact nature of this deficit remains unclear. The present study examined motor programming in AOS in the context of a recent two-stage model [Klapp, S. T. (1995). Motor response programming during simple and choice reaction time: The role of practice. "Journal of…

  13. Agricultural Electricity. Electric Motors. Student Manual.

    Science.gov (United States)

    Benson, Robert T.

    Addressed to the student, this manual, which includes supplementary diagrams, discusses the following topics and principles: Electromagnetic fields, electromagnets, parts of an electric motor, determining speed of an electric motor, types of electric motors in common use (split-phase, capacitor, repulsion-induction, three-phase), the electric…

  14. Control of a superconducting synchronous motor

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, Y; Pei, R; Jiang, Q; Hong, Z; Coombs, T A [Engineering Department, Cambridge University, Trumpington Street, Cambridge CB2 1PZ (United Kingdom)

    2007-04-15

    This paper presents a control algorithm for starting up a high temperature superconducting synchronous motor. The mathematical model of the motor has been established in m-file in Matlab and the parameters have been identified by means of the finite-element analysis method. Different starting methods for the motor have been compared and discussed, and eventually a hybrid control algorithm is proposed.

  15. Motor Development Programming in Trisomic-21 Babies

    Science.gov (United States)

    Sanz, Teresa; Menendez, Javier; Rosique, Teresa

    2011-01-01

    The present study contributes to the understanding of gross motor development in babies with Down's syndrome. Also, it facilitates the comprehension of the efficiency of the early motor stimulation as well as of beginning it as early as possible. We worked with two groups of babies with Down's syndrome, beginning the early motor training in each…

  16. Electric Motor Thermal Management R&D

    Energy Technology Data Exchange (ETDEWEB)

    Bennion, Kevin

    2016-06-07

    Thermal management enables more efficient and cost-effective motors. This Annual Merit Review presentation describes the technical accomplishments and progress in electric motor thermal management R&D over the last year. This project supports a broad industry demand for data, analysis methods, and experimental techniques to improve and better understand motor thermal management.

  17. [Non-motor symptoms of Parkinson's disease

    NARCIS (Netherlands)

    Weerkamp, N.J.; Nijhof, A.; Tissingh, G.

    2012-01-01

    Parkinson's disease has traditionally been viewed as a disease with only motor features. Nowadays, a wide variety of non-motor symptoms and signs are also recognised as being characteristic of the disease. Non-motor symptoms, most importantly autonomic dysfunction, neuropsychiatric symptoms and

  18. Motor performance in children with Noonan syndrome

    NARCIS (Netherlands)

    Croonen, E.A.; Essink, M.; Burgt, I. van der; Draaisma, J.M.; Noordam, C.; Nijhuis-Van der Sanden, M.W.G.

    2017-01-01

    Although problems with motor performance in daily life are frequently mentioned in Noonan syndrome, the motor performance profile has never been systematically investigated. The aim of this study was to examine whether a specific profile in motor performance in children with Noonan syndrome was seen

  19. 33 CFR 127.1311 - Motor vehicles.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Motor vehicles. 127.1311 Section... Waterfront Facilities Handling Liquefied Hazardous Gas Operations § 127.1311 Motor vehicles. (a) When LHG is... operator shall ensure that no person— (1) Stops or parks a motor vehicle in a space other than a designated...

  20. 33 CFR 159.69 - Motor ratings.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Motor ratings. 159.69 Section 159.69 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) POLLUTION MARINE SANITATION DEVICES Design, Construction, and Testing § 159.69 Motor ratings. Motors must be rated...

  1. 47 CFR 32.2112 - Motor vehicles.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Motor vehicles. 32.2112 Section 32.2112... FOR TELECOMMUNICATIONS COMPANIES Instructions for Balance Sheet Accounts § 32.2112 Motor vehicles. This account shall include the original cost of motor vehicles of the type which are designed and...

  2. 76 FR 32390 - Motor Carrier Safety Advisory Committee Public Meeting

    Science.gov (United States)

    2011-06-06

    ... DEPARTMENT OF TRANSPORTATION Federal Motor Carrier Safety Administration [Docket No. FMCSA-2006-26367] Motor Carrier Safety Advisory Committee Public Meeting AGENCY: Federal Motor Carrier Safety Administration (FMCSA), DOT. ACTION: Notice of Motor Carrier Safety Advisory Committee (MCSAC) Meeting. SUMMARY...

  3. 77 FR 46555 - Motor Carrier Safety Advisory Committee: Public Meeting

    Science.gov (United States)

    2012-08-03

    ... DEPARTMENT OF TRANSPORTATION Federal Motor Carrier Safety Administration [Docket No. FMCSA-2006-26367] Motor Carrier Safety Advisory Committee: Public Meeting AGENCY: Federal Motor Carrier Safety Administration (FMCSA), DOT. ACTION: Notice of meeting of Motor Carrier Safety Advisory Committee (MCSAC...

  4. 75 FR 2923 - Motor Carrier Safety Advisory Committee Public Meeting

    Science.gov (United States)

    2010-01-19

    ... DEPARTMENT OF TRANSPORTATION Federal Motor Carrier Safety Administration [Docket No. FMCSA-2006-26367] Motor Carrier Safety Advisory Committee Public Meeting AGENCY: Federal Motor Carrier Safety Administration (FMCSA), DOT. ACTION: Notice of Motor Carrier Safety Advisory Committee Meeting. SUMMARY: FMCSA...

  5. 75 FR 29384 - Motor Carrier Safety Advisory Committee Public Meeting

    Science.gov (United States)

    2010-05-25

    ... DEPARTMENT OF TRANSPORTATION Federal Motor Carrier Safety Administration [Docket No. FMCSA-2010-0143] Motor Carrier Safety Advisory Committee Public Meeting AGENCY: Federal Motor Carrier Safety Administration (FMCSA), DOT. ACTION: Notice of Motor Carrier Safety Advisory Committee meeting. SUMMARY: FMCSA...

  6. 75 FR 72863 - Motor Carrier Safety Advisory Committee Public Meeting

    Science.gov (United States)

    2010-11-26

    ... DEPARTMENT OF TRANSPORTATION Federal Motor Carrier Safety Administration [Docket No. FMCSA-2006-26367] Motor Carrier Safety Advisory Committee Public Meeting AGENCY: Federal Motor Carrier Safety Administration, DOT. ACTION: Notice of Motor Carrier Safety Advisory Committee Meeting. SUMMARY: FMCSA announces...

  7. 75 FR 50797 - Motor Carrier Safety Advisory Committee Public Meeting

    Science.gov (United States)

    2010-08-17

    ... DEPARTMENT OF TRANSPORTATION Federal Motor Carrier Safety Administration [Docket No. FMCSA-2010-0143] Motor Carrier Safety Advisory Committee Public Meeting AGENCY: Federal Motor Carrier Safety Administration (FMCSA), DOT. ACTION: Notice of Motor Carrier Safety Advisory Committee Meeting. SUMMARY: FMCSA...

  8. Pre-motor and motor activities in early handwriting

    OpenAIRE

    van Zwieten, Koos Jaap

    2011-01-01

    Behavioural studies make use of handwritten letters’ characteristics like strokes, roundedness, etcetera. In consequence, Fisher et al. (2010) studying brain activation during rejected love, noticed typical pre-motor activity patterns, as suggested by irregular writing patterns as well, due to basal ganglia dysfunction (Mergl et al., 2004). A short historical text written in a presumably depressed mood was checked on such characteristics in the light of hypothesised finger-, and hand movement...

  9. DEVELOPMENT OF FINE MOTOR COORDINATION AND VISUAL-MOTOR INTEGRATION IN PRESCHOOL CHILDREN

    OpenAIRE

    MEMISEVIC Haris; HADZIC Selmir

    2015-01-01

    Fine motor skills are prerequisite for many everyday activities and they are a good predictor of a child's later academic outcome. The goal of the present study was to assess the effects of age on the development of fine motor coordination and visual-motor integration in preschool children. The sample for this study consisted of 276 preschool children from Canton Sara­jevo, Bosnia and Herzegovina. We assessed children's motor skills with Beery Visual Motor Integration Test and Lafayette Pegbo...

  10. Maximum power operation of interacting molecular motors

    DEFF Research Database (Denmark)

    Golubeva, Natalia; Imparato, Alberto

    2013-01-01

    , as compared to the non-interacting system, in a wide range of biologically compatible scenarios. We furthermore consider the case where the motor-motor interaction directly affects the internal chemical cycle and investigate the effect on the system dynamics and thermodynamics.......We study the mechanical and thermodynamic properties of different traffic models for kinesin which are relevant in biological and experimental contexts. We find that motor-motor interactions play a fundamental role by enhancing the thermodynamic efficiency at maximum power of the motors...

  11. Motor control is decision-making.

    Science.gov (United States)

    Wolpert, Daniel M; Landy, Michael S

    2012-12-01

    Motor behavior may be viewed as a problem of maximizing the utility of movement outcome in the face of sensory, motor and task uncertainty. Viewed in this way, and allowing for the availability of prior knowledge in the form of a probability distribution over possible states of the world, the choice of a movement plan and strategy for motor control becomes an application of statistical decision theory. This point of view has proven successful in recent years in accounting for movement under risk, inferring the loss function used in motor tasks, and explaining motor behavior in a wide variety of circumstances. Copyright © 2012 Elsevier Ltd. All rights reserved.

  12. Mathematical Modeling of Diaphragm Pneumatic Motors

    Directory of Open Access Journals (Sweden)

    Fojtášek Kamil

    2014-03-01

    Full Text Available Pneumatic diaphragm motors belong to the group of motors with elastic working parts. This part is usually made of rubber with a textile insert and it is deformed under the pressure of a compressed air or from the external mass load. This is resulting in a final working effect. In this type of motors are in contact two different elastic environments – the compressed air and the esaltic part. These motors are mainly the low-stroke and working with relatively large forces. This paper presents mathematical modeling static properties of diaphragm motors.

  13. Motor Skill Competence and Perceived Motor Competence: Which Best Predicts Physical Activity among Girls?

    Science.gov (United States)

    Khodaverdi, Zeinab; Bahram, Abbas; Khalaji, Hassan; Kazemnejad, Anoshirvan

    2013-10-01

    The main purpose of this study was to determine which correlate, perceived motor competence or motor skill competence, best predicts girls' physical activity behavior. A sample of 352 girls (mean age=8.7, SD=0.3 yr) participated in this study. To assess motor skill competence and perceived motor competence, each child completed the Test of Gross Motor Development-2 and Physical Ability sub-scale of Marsh's Self-Description Questionnaire. Children's physical activity was assessed by the Physical Activity Questionnaire for Older Children. Multiple linear regression model was used to determine whether perceived motor competence or motor skill competence best predicts moderate-to-vigorous self-report physical activity. Multiple regression analysis indicated that motor skill competence and perceived motor competence predicted 21% variance in physical activity (R(2)=0.21, F=48.9, P=0.001), and motor skill competence (R(2)=0.15, ᵝ=0.33, P= 0.001) resulted in more variance than perceived motor competence (R(2)=0.06, ᵝ=0.25, P=0.001) in physical activity. Results revealed motor skill competence had more influence in comparison with perceived motor competence on physical activity level. We suggest interventional programs based on motor skill competence and perceived motor competence should be administered or implemented to promote physical activity in young girls.

  14. Motor-sensory confluence in tactile perception.

    Science.gov (United States)

    Saig, Avraham; Gordon, Goren; Assa, Eldad; Arieli, Amos; Ahissar, Ehud

    2012-10-03

    Perception involves motor control of sensory organs. However, the dynamics underlying emergence of perception from motor-sensory interactions are not yet known. Two extreme possibilities are as follows: (1) motor and sensory signals interact within an open-loop scheme in which motor signals determine sensory sampling but are not affected by sensory processing and (2) motor and sensory signals are affected by each other within a closed-loop scheme. We studied the scheme of motor-sensory interactions in humans using a novel object localization task that enabled monitoring the relevant overt motor and sensory variables. We found that motor variables were dynamically controlled within each perceptual trial, such that they gradually converged to steady values. Training on this task resulted in improvement in perceptual acuity, which was achieved solely by changes in motor variables, without any change in the acuity of sensory readout. The within-trial dynamics is captured by a hierarchical closed-loop model in which lower loops actively maintain constant sensory coding, and higher loops maintain constant sensory update flow. These findings demonstrate interchangeability of motor and sensory variables in perception, motor convergence during perception, and a consistent hierarchical closed-loop perceptual model.

  15. Motor carrier evaluation program plan

    International Nuclear Information System (INIS)

    Portsmouth, J.H.; Maxwell, J.E.; Boness, G.O.; Rice, L.E.

    1991-04-01

    The US Department of Energy (DOE) Transportation Management Program (TMP) has established a program to assist the DOE field offices and their contractors in evaluating the motor carriers used to transport DOE-owned hazardous and radioactive materials. This program was initiated to provide the DOE field offices with the tools necessary to help ensure, during this period of motor carrier deregulation, that only highly qualified carriers transport radioactive and hazardous commodities for the DOE. This program will assist DOE in maintaining their excellent performance record in the safe transportation of hazardous commodities. The program was also developed in response to public concern surrounding the transportation of hazardous materials. Representatives of other federal agencies, states, and tribal governments, as well as the news media, have expressed concern about the selection and qualification of carriers engaged in the transportation of Highway Route-Controlled Quantities (HRCQ) and Truckload (TL) quantities of radioactive material for the DOE. 8 refs

  16. Wet motor geroter fuel pump

    Energy Technology Data Exchange (ETDEWEB)

    Wiernicki, M.V.

    1987-05-05

    This patent describes a wet motor gerotor fuel pump for pumping fuel from a fuel source to an internal combustion which consists of: gerotor pump means comprising an inner pump gear, an outer pump gear, and second tang means located on one of the inner and outer pump gears. The second tang means further extends in a second radial direction radially offset from the first radial direction and forms a driving connection with the first tang means such that the fuel pump pumps fuel from the fuel source into the narrow conduit inlet chamber, through the gerotor pump means past the electric motor means into the outlet housing means substantially along the flow axis to the internal combustion engine.

  17. Online Monitoring of Induction Motors

    Energy Technology Data Exchange (ETDEWEB)

    McJunkin, Timothy R. [Idaho National Lab. (INL), Idaho Falls, ID (United States); Agarwal, Vivek [Idaho National Lab. (INL), Idaho Falls, ID (United States); Lybeck, Nancy Jean [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2016-01-01

    The online monitoring of active components project, under the Advanced Instrumentation, Information, and Control Technologies Pathway of the Light Water Reactor Sustainability Program, researched diagnostic and prognostic models for alternating current induction motors (IM). Idaho National Laboratory (INL) worked with the Electric Power Research Institute (EPRI) to augment and revise the fault signatures previously implemented in the Asset Fault Signature Database of EPRI’s Fleet Wide Prognostic and Health Management (FW PHM) Suite software. Induction Motor diagnostic models were researched using the experimental data collected by Idaho State University. Prognostic models were explored in the set of literature and through a limited experiment with 40HP to seek the Remaining Useful Life Database of the FW PHM Suite.

  18. Validating the Rett Syndrome Gross Motor Scale

    DEFF Research Database (Denmark)

    Downs, Jenny; Stahlhut, Michelle; Wong, Kingsley

    2016-01-01

    .93-0.98). The standard error of measurement for the total score was 2 points and we would be 95% confident that a change 4 points in the 45-point scale would be greater than within-subject measurement error. The Rett Syndrome Gross Motor Scale could be an appropriate measure of gross motor skills in clinical practice......Rett syndrome is a pervasive neurodevelopmental disorder associated with a pathogenic mutation on the MECP2 gene. Impaired movement is a fundamental component and the Rett Syndrome Gross Motor Scale was developed to measure gross motor abilities in this population. The current study investigated...... the validity and reliability of the Rett Syndrome Gross Motor Scale. Video data showing gross motor abilities supplemented with parent report data was collected for 255 girls and women registered with the Australian Rett Syndrome Database, and the factor structure and relationships between motor scores, age...

  19. Motor memory in sports success

    Directory of Open Access Journals (Sweden)

    Silvia GRĂDINARU

    2017-02-01

    Full Text Available The model of modern sports performance asks for certain graduation in the treatment of its efficiency. Besides the coaching model, what matters is the genetic potential of the child or junior, and particularly the selection of the young talented athlete identified at the proper time and included in a proper training system, in full harmony with the education process. The sports output is determined by the simultaneous action of several factors whose influences are different. At present, there is a tendency to improve those factors on which rely sports outcomes and that need to be analysed and selected. Psychic capacity is a major factor, and mental control – the power to focus, motor intelligence, motor memory, creativity, and tactical skills play a major role in an athlete’s style. This study aims at showing the measure in which motor memory allows early and reliable diagnosis of future performance. The subjects selected are components of the mini-basket team of the Sports Club “Sport Star” from Timisoara, little girls that have played basketball since 1st grade in their free time (some of the girls have played it for four years. The research was carried out during a competitive year; we monitored the subjects both during coach lessons and minibasketball championship. To assess motor memory, we used the “cerebral module” consisting in memorising a complex of technical and tactical elements and applying them depending on the situation in the field. The research also involved monitoring the subjects in four directions considered defining in the assessment of the young athletes: somatic data, physical features, basketball features and intellectual potential. Most parameters point out a medium homogeneity of the group, except for height and commitment (great homogeneity. Half of the athletes of the tested group are above the mean of the group, which allows guiding them towards higher coaching forms (allowing them to practice basketball

  20. Hermetically sealed superconducting magnet motor

    Science.gov (United States)

    DeVault, Robert C.; McConnell, Benjamin W.; Phillips, Benjamin A.

    1996-01-01

    A hermetically sealed superconducting magnet motor includes a rotor separated from a stator by either a radial gap, an axial gap, or a combined axial and radial gap. Dual conically shaped stators are used in one embodiment to levitate a disc-shaped rotor made of superconducting material within a conduit for moving cryogenic fluid. As the rotor is caused to rotate when the field stator is energized, the fluid is pumped through the conduit.

  1. Advances in esophageal motor disorders.

    Science.gov (United States)

    Smout, André Jpm

    2008-07-01

    Esophageal motor disorders, often leading to dysphagia and chest pain, continue to pose diagnostic and therapeutic problems. In the past 12 months important new information regarding esophageal motor disorders was published. This information will be reviewed in this paper. A number of studies have addressed the issue of heterogeneity in achalasia, the best defined esophageal motility disorder. The spastic esophageal motility disorders nutcracker esophagus and diffuse esophageal spasm may coexist with gastroesophageal reflux disease, which has consequences for the management of patients with these disorders. The entity labelled ineffective esophageal motility is associated with reflux esophagitis, but also with morbid obesity. For the detection of disordered transit caused by ineffective esophageal motility, application of intraluminal impedance monitoring in conjunction with manometry leads to improved diagnosis. New data on the effect of Nissen fundoplication on esophageal motility were published during the last year. Recent knowledge on the heterogeneity of achalasia and the association of spastic esophageal motor disorders and ineffective motility with reflux disease will help the clinician in the management of patients with these disorders.

  2. Summary: High Temperature Downhole Motor

    Energy Technology Data Exchange (ETDEWEB)

    Raymond, David W. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2017-10-01

    Directional drilling can be used to enable multi-lateral completions from a single well pad to improve well productivity and decrease environmental impact. Downhole rotation is typically developed with a motor in the Bottom Hole Assembly (BHA) that develops drilling power (speed and torque) necessary to drive rock reduction mechanisms (i.e., the bit) apart from the rotation developed by the surface rig. Historically, wellbore deviation has been introduced by a “bent-sub,” located in the BHA, that introduces a small angular deviation, typically less than 3 degrees, to allow the bit to drill off-axis with orientation of the BHA controlled at the surface. The development of a high temperature downhole motor would allow reliable use of bent subs for geothermal directional drilling. Sandia National Laboratories is pursuing the development of a high temperature motor that will operate on either drilling fluid (water-based mud) or compressed air to enable drilling high temperature, high strength, fractured rock. The project consists of designing a power section based upon geothermal drilling requirements; modeling and analysis of potential solutions; and design, development and testing of prototype hardware to validate the concept. Drilling costs contribute substantially to geothermal electricity production costs. The present development will result in more reliable access to deep, hot geothermal resources and allow preferential wellbore trajectories to be achieved. This will enable development of geothermal wells with multi-lateral completions resulting in improved geothermal resource recovery, decreased environmental impact and enhanced well construction economics.

  3. Motor Proficiency in Young Children

    Directory of Open Access Journals (Sweden)

    Fotini Venetsanou

    2016-01-01

    Full Text Available This study aimed to examine motor proficiency in young children, focusing on potential gender differences. For that purpose, the Bruininks-Oseretsky Test of Motor Proficiency–Long Form (BOTMP-LF was administered to 540 children (272 boys, 4½ to 6 years old. First, the 2 (sex × 4 (age groups ANOVA computed on children’s total BOTMP-LF scores showed that age had a statistically significant effect, whereas gender did not. Second, the one-way MANCOVA applied on subtest scores, with age as covariate, revealed statistical significant gender differences; however, η2 values were found to be small or moderate. Finally, the MANCOVA applied on items where significant gender differences have been reported showed a significant effect of gender. Nonetheless, η2 values exceeded the limit of practical significance only on two items (“standing on preferred leg on floor”, “throwing a ball at a target with preferred hand” that are associated with gender-stereotyped activities. It can be concluded that (a besides statistical significance, effect sizes should be examined for the results of a study to be adequately interpreted; (b young boys’ and girls’ motor proficiency is similar rather than different. Gender differences in specific skills should be used for movement programs to be individualized.

  4. What happens to the motor theory of perception when the motor system is damaged?

    Science.gov (United States)

    Stasenko, Alena; Garcea, Frank E; Mahon, Bradford Z

    2013-09-01

    Motor theories of perception posit that motor information is necessary for successful recognition of actions. Perhaps the most well known of this class of proposals is the motor theory of speech perception, which argues that speech recognition is fundamentally a process of identifying the articulatory gestures (i.e. motor representations) that were used to produce the speech signal. Here we review neuropsychological evidence from patients with damage to the motor system, in the context of motor theories of perception applied to both manual actions and speech. Motor theories of perception predict that patients with motor impairments will have impairments for action recognition. Contrary to that prediction, the available neuropsychological evidence indicates that recognition can be spared despite profound impairments to production. These data falsify strong forms of the motor theory of perception, and frame new questions about the dynamical interactions that govern how information is exchanged between input and output systems.

  5. Sequence Variations in the Flagellar Antigen Genes fliCH25 and fliCH28 of Escherichia coli and Their Use in Identification and Characterization of Enterohemorrhagic E. coli (EHEC O145:H25 and O145:H28.

    Directory of Open Access Journals (Sweden)

    Lothar Beutin

    Full Text Available Enterohemorrhagic E. coli (EHEC serogroup O145 is regarded as one of the major EHEC serogroups involved in severe infections in humans. EHEC O145 encompasses motile and non-motile strains of serotypes O145:H25 and O145:H28. Sequencing the fliC-genes associated with the flagellar antigens H25 and H28 revealed the genetic diversity of the fliCH25 and fliCH28 gene sequences in E. coli. Based on allele discrimination of these fliC-genes real-time PCR tests were designed for identification of EHEC O145:H25 and O145:H28. The fliCH25 genes present in O145:H25 were found to be very similar to those present in E. coli serogroups O2, O100, O165, O172 and O177 pointing to their common evolution but were different from fliCH25 genes of a multiple number of other E. coli serotypes. In a similar way, EHEC O145:H28 harbor a characteristic fliCH28 allele which, apart from EHEC O145:H28, was only found in enteropathogenic (EPEC O28:H28 strains that shared some common traits with EHEC O145:H28. The real time PCR-assays targeting these fliCH25[O145] and fliCH28[O145] alleles allow better characterization of EHEC O145:H25 and EHEC O145:H28. Evaluation of these PCR assays in spiked ready-to eat salad samples resulted in specific detection of both types of EHEC O145 strains even when low spiking levels of 1-10 cfu/g were used. Furthermore these PCR assays allowed identification of non-motile E. coli strains which are serologically not typable for their H-antigens. The combined use of O-antigen genotyping (O145wzy and detection of the respective fliCH25[O145] and fliCH28[O145] allele types contributes to improve identification and molecular serotyping of E. coli O145 isolates.

  6. Genetic Diversity of the fliC Genes Encoding the Flagellar Antigen H19 of Escherichia coli and Application to the Specific Identification of Enterohemorrhagic E. coli O121:H19

    Science.gov (United States)

    Beutin, Lothar; Delannoy, Sabine

    2015-01-01

    Enterohemorrhagic Escherichia coli (EHEC) O121:H19 belong to a specific clonal type distinct from other classical EHEC and major enteropathogenic E. coli groups and is regarded as one of the major EHEC serogroups involved in severe infections in humans. Sequencing of the fliC genes associated with the flagellar antigen H19 (fliCH19) revealed the genetic diversity of the fliCH19 gene sequences in E. coli. A cluster analysis of 12 fliCH19 sequences, 4 from O121 and 8 from non-O121 E. coli strains, revealed five different genotypes. All O121:H19 strains fell into one cluster, whereas a second cluster was formed by five non-O121:H19 strains. Cluster 1 and cluster 2 strains differ by 27 single nucleotide exchanges in their fliCH19 genes (98.5% homology). Based on allele discrimination of the fliCH19 genes, a real-time PCR test was designed for specific identification of EHEC O121:H19. The O121 fliCH19 PCR tested negative in 73 E. coli H19 strains that belonged to serogroups other than O121, including 28 different O groups, O-nontypeable H19, and O-rough:H19 strains. The O121 fliCH19 PCR reacted with all 16 tested O121:H19 strains and 1 O-rough:H19 strain which was positive for the O121 wzx gene. A cross-reaction was observed only with E. coli H32 strains which share sequence similarities in the target region of the O121 fliCH19 PCR. The combined use of O-antigen genotyping (O121 wzx) and the detection of O121 fliCH19 allele type contributes to improving the identification and molecular serotyping of EHEC O121:H19 motile and nonmotile strains and variants of these strains lacking stx genes. PMID:25862232

  7. Recruitment of rat diaphragm motor units across motor behaviors with different levels of diaphragm activation.

    Science.gov (United States)

    Seven, Yasin B; Mantilla, Carlos B; Sieck, Gary C

    2014-12-01

    Phrenic motor neurons are recruited across a range of motor behaviors to generate varying levels of diaphragm muscle (DIAm) force. We hypothesized that DIAm motor units are recruited in a fixed order across a range of motor behaviors of varying force levels, consistent with the Henneman Size Principle. Single motor unit action potentials and compound DIAm EMG activities were recorded in anesthetized, neurally intact rats across different motor behaviors, i.e., eupnea, hypoxia-hypercapnia (10% O2 and 5% CO2), deep breaths, sustained airway occlusion, and sneezing. Central drive [estimated by root-mean-squared (RMS) EMG value 75 ms after the onset of EMG activity (RMS75)], recruitment delay, and onset discharge frequencies were similar during eupnea and hypoxia-hypercapnia. Compared with eupnea, central drive increased (∼25%) during deep breaths, and motor units were recruited ∼12 ms earlier (P motor units were recruited ∼30 ms earlier (P motor unit onset discharge frequencies were significantly higher (P Recruitment order of motor unit pairs observed during eupnea was maintained for 98%, 87%, and 84% of the same pairs recorded during hypoxia-hypercapnia, deep breaths, and airway occlusion, respectively. Reversals in motor unit recruitment order were observed primarily if motor unit pairs were recruited motor unit recruitment order being determined primarily by the intrinsic size-dependent electrophysiological properties of phrenic motor neurons. Copyright © 2014 the American Physiological Society.

  8. Using the motor to monitor pump conditions

    Energy Technology Data Exchange (ETDEWEB)

    Casada, D. [Oak Ridge National Lab., TN (United States)

    1996-12-01

    When the load of a mechanical device being driven by a motor changes, whether in response to changes in the overall process or changes in the performance of the driven device, the motor inherently responds. For induction motors, the current amplitude and phase angle change as the shaft load changes. By examining the details of these changes in amplitude and phase, load fluctuations of the driven device can be observed. The usefulness of the motor as a transducer to improve the understanding of devices with high torque fluctuations, such as positive displacement compressors and motor-operated valves, has been recognized and demonstrated for a number of years. On such devices as these, the spectrum of the motor current amplitude, phase, or power normally has certain characteristic peaks associated with various load components, such as the piston stroke or gear tooth meshing frequencies. Comparison and trending of the amplitudes of these peaks has been shown to provide some indication of their mechanical condition. For most centrifugal pumps, the load fluctuations are normally low in torque amplitude, and as a result, the motor experiences a correspondingly lower level of load fluctuation. However, both laboratory and field test data have demonstrated that the motor does provide insight into some important pump performance conditions, such as hydraulic stability and pump-to-motor alignment. Comparisons of other dynamic signals, such as vibration and pressure pulsation, to motor data for centrifugal pumps are provided. The effects of inadequate suction head, misalignment, mechanical and hydraulic unbalance on these signals are presented.

  9. Using the motor to monitor pump conditions

    International Nuclear Information System (INIS)

    Casada, D.

    1996-01-01

    When the load of a mechanical device being driven by a motor changes, whether in response to changes in the overall process or changes in the performance of the driven device, the motor inherently responds. For induction motors, the current amplitude and phase angle change as the shaft load changes. By examining the details of these changes in amplitude and phase, load fluctuations of the driven device can be observed. The usefulness of the motor as a transducer to improve the understanding of devices with high torque fluctuations, such as positive displacement compressors and motor-operated valves, has been recognized and demonstrated for a number of years. On such devices as these, the spectrum of the motor current amplitude, phase, or power normally has certain characteristic peaks associated with various load components, such as the piston stroke or gear tooth meshing frequencies. Comparison and trending of the amplitudes of these peaks has been shown to provide some indication of their mechanical condition. For most centrifugal pumps, the load fluctuations are normally low in torque amplitude, and as a result, the motor experiences a correspondingly lower level of load fluctuation. However, both laboratory and field test data have demonstrated that the motor does provide insight into some important pump performance conditions, such as hydraulic stability and pump-to-motor alignment. Comparisons of other dynamic signals, such as vibration and pressure pulsation, to motor data for centrifugal pumps are provided. The effects of inadequate suction head, misalignment, mechanical and hydraulic unbalance on these signals are presented

  10. Tokyo Motor Show 2003; Tokyo Motor Show 2003

    Energy Technology Data Exchange (ETDEWEB)

    Joly, E.

    2004-01-01

    The text which follows present the different techniques exposed during the 37. Tokyo Motor Show. The report points out the great tendencies of developments of the Japanese automobile industry. The hybrid electric-powered vehicles or those equipped with fuel cells have been highlighted by the Japanese manufacturers which allow considerable budgets in the research of less polluting vehicles. The exposed models, although being all different according to the manufacturer, use always a hybrid system: fuel cell/battery. The manufacturers have stressed too on the intelligent systems for navigation and safety as well as on the design and comfort. (O.M.)

  11. Interference in motor learning - is motor interference sensory?

    DEFF Research Database (Denmark)

    Jensen, Jesper Lundbye; Petersen, Tue Hvass; Rothwell, John C

    mechanisms determine whether or not interference occurs. We hypothesised that interference requires the same neural circuits to be engaged in the two tasks and provoke competing processes of synaptic plasticity. To test this, subjects learned a ballistic ankle plantarflexion task. Early motor memory...... was disrupted by subsequent learning of a precision tracking task with the same agonist muscle group, but not by learning involving antagonist muscles or by voluntary agonist contractions that did not require learning. If the competing task was learned with the same agonist muscle group 4 hours following...

  12. Apraxia and motor dysfunction in corticobasal syndrome.

    Directory of Open Access Journals (Sweden)

    James R Burrell

    Full Text Available BACKGROUND: Corticobasal syndrome (CBS is characterized by multifaceted motor system dysfunction and cognitive disturbance; distinctive clinical features include limb apraxia and visuospatial dysfunction. Transcranial magnetic stimulation (TMS has been used to study motor system dysfunction in CBS, but the relationship of TMS parameters to clinical features has not been studied. The present study explored several hypotheses; firstly, that limb apraxia may be partly due to visuospatial impairment in CBS. Secondly, that motor system dysfunction can be demonstrated in CBS, using threshold-tracking TMS, and is linked to limb apraxia. Finally, that atrophy of the primary motor cortex, studied using voxel-based morphometry analysis (VBM, is associated with motor system dysfunction and limb apraxia in CBS. METHODS: Imitation of meaningful and meaningless hand gestures was graded to assess limb apraxia, while cognitive performance was assessed using the Addenbrooke's Cognitive Examination - Revised (ACE-R, with particular emphasis placed on the visuospatial subtask. Patients underwent TMS, to assess cortical function, and VBM. RESULTS: In total, 17 patients with CBS (7 male, 10 female; mean age 64.4+/- 6.6 years were studied and compared to 17 matched control subjects. Of the CBS patients, 23.5% had a relatively inexcitable motor cortex, with evidence of cortical dysfunction in the remaining 76.5% patients. Reduced resting motor threshold, and visuospatial performance, correlated with limb apraxia. Patients with a resting motor threshold <50% performed significantly worse on the visuospatial sub-task of the ACE-R than other CBS patients. Cortical function correlated with atrophy of the primary and pre-motor cortices, and the thalamus, while apraxia correlated with atrophy of the pre-motor and parietal cortices. CONCLUSIONS: Cortical dysfunction appears to underlie the core clinical features of CBS, and is associated with atrophy of the primary motor and

  13. Motor-pump unit provided with a lifting appliance of the motor

    International Nuclear Information System (INIS)

    Veronesi, Luciano; Francis, W.R.

    1978-01-01

    This invention relates to lifting appliances and particularly concerns a 'pump and motor set' or motor-pump unit fitted with a lifting appliance enabling the motor to be separated from the pump. In nuclear power stations the reactor discharges heat that is carried by the coolant to a distant point away from the reactor to generate steam and electricity conventionally. In order to cause the reactor coolant to flow through the system, coolant motor-pump units are provided in the cooling system. These units are generally of the vertical type with an electric motor fitted vertically on the pump by means of a cylindrical or conical structure called motor support [fr

  14. Reduction of power consumption in motor-driven applications by using PM motors; PM = Permanent Magnet; Reduktion af elforbrug til motordrift ved anvendelse af PM motorer

    Energy Technology Data Exchange (ETDEWEB)

    Hvenegaard, C.M.; Hansen, Mads P.R.; Groenborg Nikolaisen, C. (Teknologisk Institut, Taastrup (Denmark)); Nielsen, Sandie B. (Teknologisk Institut, AArhus (Denmark)); Ritchie, E.; Leban, K. (Aalborg Univ., Aalborg (Denmark))

    2009-12-15

    The traditional asynchronous motor with aluminum rotor is today by far the most widespread and sold electric motor, but a new and more energy efficient type of engine - the permanent magnet motor (PM motor) - is expected in the coming years to win larger and larger market shares. Several engine manufacturers in Europe, USA and Asia are now beginning to market the PM motors, which can replace the traditional asynchronous motor. The project aims to uncover the pros and cons of replacing asynchronous motors including EFF1 engines with PM motors, including the price difference. Furthermore, it is identified how the efficiency of PM motors is affected by low load levels and at various forms of control. Finally, the energy savings potential is analysed, by replacing asynchronous motors with PM motors. The study includes laboratory tests of PM motors, made in a test stand at Danish Technological Institute. (ln)

  15. Concurrent word generation and motor performance: further evidence for language-motor interaction.

    Directory of Open Access Journals (Sweden)

    Amy D Rodriguez

    Full Text Available Embodied/modality-specific theories of semantic memory propose that sensorimotor representations play an important role in perception and action. A large body of evidence supports the notion that concepts involving human motor action (i.e., semantic-motor representations are processed in both language and motor regions of the brain. However, most studies have focused on perceptual tasks, leaving unanswered questions about language-motor interaction during production tasks. Thus, we investigated the effects of shared semantic-motor representations on concurrent language and motor production tasks in healthy young adults, manipulating the semantic task (motor-related vs. nonmotor-related words and the motor task (i.e., standing still and finger-tapping. In Experiment 1 (n = 20, we demonstrated that motor-related word generation was sufficient to affect postural control. In Experiment 2 (n = 40, we demonstrated that motor-related word generation was sufficient to facilitate word generation and finger tapping. We conclude that engaging semantic-motor representations can have a reciprocal influence on motor and language production. Our study provides additional support for functional language-motor interaction, as well as embodied/modality-specific theories.

  16. Parkinson's Disease Subtypes Identified from Cluster Analysis of Motor and Non-motor Symptoms.

    Science.gov (United States)

    Mu, Jesse; Chaudhuri, Kallol R; Bielza, Concha; de Pedro-Cuesta, Jesus; Larrañaga, Pedro; Martinez-Martin, Pablo

    2017-01-01

    Parkinson's disease is now considered a complex, multi-peptide, central, and peripheral nervous system disorder with considerable clinical heterogeneity. Non-motor symptoms play a key role in the trajectory of Parkinson's disease, from prodromal premotor to end stages. To understand the clinical heterogeneity of Parkinson's disease, this study used cluster analysis to search for subtypes from a large, multi-center, international, and well-characterized cohort of Parkinson's disease patients across all motor stages, using a combination of cardinal motor features (bradykinesia, rigidity, tremor, axial signs) and, for the first time, specific validated rater-based non-motor symptom scales. Two independent international cohort studies were used: (a) the validation study of the Non-Motor Symptoms Scale ( n = 411) and (b) baseline data from the global Non-Motor International Longitudinal Study ( n = 540). k -means cluster analyses were performed on the non-motor and motor domains (domains clustering) and the 30 individual non-motor symptoms alone (symptoms clustering), and hierarchical agglomerative clustering was performed to group symptoms together. Four clusters are identified from the domains clustering supporting previous studies: mild, non-motor dominant, motor-dominant, and severe. In addition, six new smaller clusters are identified from the symptoms clustering, each characterized by clinically-relevant non-motor symptoms. The clusters identified in this study present statistical confirmation of the increasingly important role of non-motor symptoms (NMS) in Parkinson's disease heterogeneity and take steps toward subtype-specific treatment packages.

  17. Skeletal maturation, fundamental motor skills and motor coordination in children 7-10 years.

    Science.gov (United States)

    Freitas, Duarte L; Lausen, Berthold; Maia, José António; Lefevre, Johan; Gouveia, Élvio Rúbio; Thomis, Martine; Antunes, António Manuel; Claessens, Albrecht L; Beunen, Gaston; Malina, Robert M

    2015-01-01

    Relationships between skeletal maturation and fundamental motor skills and gross motor coordination were evaluated in 429 children (213 boys and 216 girls) 7-10 years. Skeletal age was assessed (Tanner-Whitehouse 2 method), and stature, body mass, motor coordination (Körperkoordinations Test für Kinder, KTK) and fundamental motor skills (Test of Gross Motor Development, TGMD-2) were measured. Relationships among chronological age, skeletal age (expressed as the standardised residual of skeletal age on chronological age) and body size and fundamental motor skills and motor coordination were analysed with hierarchical multiple regression. Standardised residual of skeletal age on chronological age interacting with stature and body mass explained a maximum of 7.0% of the variance in fundamental motor skills and motor coordination over that attributed to body size per se. Standardised residual of skeletal age on chronological age alone accounted for a maximum of 9.0% of variance in fundamental motor skills, and motor coordination over that attributed to body size per se and interactions between standardised residual of skeletal age on chronological age and body size. In conclusion, skeletal age alone or interacting with body size has a negligible influence on fundamental motor skills and motor coordination in children 7-10 years.

  18. Control of permanent magnet synchronous motors

    CERN Document Server

    Vaez-Zadeh, Sadegh

    2018-01-01

    This is the first comprehensive, coherent, and up-to-date book devoted solely to the control of permanent magnet synchronous (PMS) motors, as the fastest growing AC motor. It covers a deep and detailed presentation of major PMS motor modeling and control methods. The readers can find rich materials on the fundamentals of PMS motor control in addition to new motor control methods, which have mainly been developed in the last two decades, including recent advancements in the field in a systematic manner. These include extensive modeling of PMS motors and a full range of vector control and direct torque control schemes, in addition to predictive control, deadbeat control, and combined control methods. All major sensorless control and parameter estimation methods are also studied. The book covers about 10 machine models in various reference frames and 70 control and estimation schemes with sufficient analytical and implementation details including about 200 original figures. A great emphasis is placed on energy-s...

  19. Error Sonification of a Complex Motor Task

    Directory of Open Access Journals (Sweden)

    Riener Robert

    2011-12-01

    Full Text Available Visual information is mainly used to master complex motor tasks. Thus, additional information providing augmented feedback should be displayed in other modalities than vision, e.g. hearing. The present work evaluated the potential of error sonification to enhance learning of a rowing-type motor task. In contrast to a control group receiving self-controlled terminal feedback, the experimental group could not significantly reduce spatial errors. Thus, motor learning was not enhanced by error sonification, although during the training the participant could benefit from it. It seems that the motor task was too slow, resulting in immediate corrections of the movement rather than in an internal representation of the general characteristics of the motor task. Therefore, further studies should elaborate the impact of error sonification when general characteristics of the motor tasks are already known.

  20. Motor Integrated Variable Speed Drives

    DEFF Research Database (Denmark)

    Singh, Yash Veer

    rectifier at the front end is presented in this thesis and requirements of a buffer stage in the form of ESI is explained in detail. An equivalent circuit and linear model are developed to give the transfer function and control of the ESI based three-phase rectifier. In this thesis a power converter...... with ESI is designed and tested with standard induction motor to verify functionality of a working drive. One modified version of the ESI based converter has also been looked into to reduce losses of converter, but because of difficulties in reducing the bus-bar inductance in that design, further...

  1. Motor performance of preschool children

    OpenAIRE

    Słonka Karina; Dyas Manuela; Słonka Tadeusz; Szurmik Tomasz

    2017-01-01

    Słonka Karina, Dyas Manuela, Słonka Tadeusz, Szurmik Tomasz. Motor performance of preschool children. Journal of Education, Health and Sport. 2017;7(8):1308-1323. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.1045272 http://ojs.ukw.edu.pl/index.php/johs/article/view/5028 https://pbn.nauka.gov.pl/sedno-webapp/works/836989 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26.01.2017...

  2. Environmental diagnosis of the washing machine motor

    DEFF Research Database (Denmark)

    Erichsen, Hanne K. Linnet

    1997-01-01

    An environmental diagnosis of the washing machine focusing on the motor is performed. The goal of the diagnosis is to designate environmental focus points in the product. The LCA of the washing machine showed impact potentials from the life cycle of the product (see: LCA of a washing machine). Th...... up 2%, Manually disassembling and recycling of metals, Reuse of motor in a new washing machine, aluminium wire instead of copper wire in the motor....

  3. Rotary steerable motor system for underground drilling

    Science.gov (United States)

    Turner, William E [Durham, CT; Perry, Carl A [Middletown, CT; Wassell, Mark E [Kingwood, TX; Barbely, Jason R [Middletown, CT; Burgess, Daniel E [Middletown, CT; Cobern, Martin E [Cheshire, CT

    2008-06-24

    A preferred embodiment of a system for rotating and guiding a drill bit in an underground bore includes a drilling motor and a drive shaft coupled to drilling motor so that drill bit can be rotated by the drilling motor. The system further includes a guidance module having an actuating arm movable between an extended position wherein the actuating arm can contact a surface of the bore and thereby exert a force on the housing of the guidance module, and a retracted position.

  4. MOTOR FUEL TAXES AND THE ENVIRONMENTAL PROTECTION

    OpenAIRE

    Michal Ptak

    2011-01-01

    Motor fuel taxes are primarily revenue-raising taxes. However, due to high fuel consumption these taxes can be quite an efficient source of general budget revenue in many countries. It seems that the taxes on motor fuels may also be useful instruments for environmental policy or climate change policy. Environmental objectives can be achieved through change of behavior of drivers. The paper presents theoretical basis for taxes levied on motor fuels. Attention is paid to the problem of external...

  5. Circuit Regulates Speed Of dc Motor

    Science.gov (United States)

    Weaver, Charles; Padden, Robin; Brown, Floyd A., Jr.

    1990-01-01

    Driving circuit regulates speed of small dc permanent-magnet motor in tape recorder. Two nested feedback loops maintain speed within 1 percent of constant value. Inner loop provides coarse regulation, while outer loop removes most of variation in speed that remains in the presence of regulation by the inner loop. Compares speed of motor with commanded speed and adjusts current supplied to motor accordingly.

  6. Design of motors for inverter operation

    Energy Technology Data Exchange (ETDEWEB)

    Haring, T. [ABB Motors OY, Vaasa (Finland)

    2000-07-01

    This paper describes very practical principles of how an induction motor should be designed for converter application. The main focus targets the efficiency of the motor and drive. The results presented are based on actual test motors and FEM-calculation simulations. FEM-calculation together with a time-stepping function is a powerful tool for estimating magnetic flux densities, iron losses, current densities and corresponding losses in windings, in other words a tool for optimisation of the motor design. Time-stepping is rather time consuming because all the circuit equations must be solved for each time-step, but it provides a way to estimate the iron losses; hysteresis and eddy current losses as well as current distribution and current losses. The calculation tool also provides the possibility to check if an existing motor is feasible for a converter drive. Alternatively if a motor is only to be supplied by a converter there are many more degrees of freedom in the electrical design and the motor may be optimised for that converter drive by incorporationg rather simple design changes. Additionally a design compromise, ''a general purpose motor'' useable for DOL and feasible for converter drive can be produced following the principles presented herewith. The converter types which are considered are indirect types and mainly voltage source converters since they are the most common on the market and are ''general purpose converters'' and providing a certain freedom to select the motor for the drive. Current source converters require ''matching'' with the motor and therefore need a precise knowledge of the motor equivalent circuit, making the selection of the motor more complicated. (orig.)

  7. 78 FR 77790 - Petition for Exemption From the Federal Motor Vehicle Theft Prevention Standard; General Motors...

    Science.gov (United States)

    2013-12-24

    ... vehicle. The antenna module translates the radio frequency signal received from the key into a digital... DEPARTMENT OF TRANSPORTATION National Highway Traffic Safety Administration Petition for Exemption From the Federal Motor Vehicle Theft Prevention Standard; General Motors Corporation AGENCY: National...

  8. The correlation between motor impairments and event-related desynchronization during motor imagery in ALS patients

    Directory of Open Access Journals (Sweden)

    Kasahara Takashi

    2012-06-01

    Full Text Available Abstract Background The event-related desynchronization (ERD in EEG is known to appear during motor imagery, and is thought to reflect cortical processing for motor preparation. The aim of this study is to examine the modulation of ERD with motor impairment in ALS patients. ERD during hand motor imagery was obtained from 8 ALS patients with a variety of motor impairments. ERD was also obtained from age-matched 11 healthy control subjects with the same motor task. The magnitude and frequency of ERD were compared between groups for characterization of ALS specific changes. Results The ERD of ALS patients were significantly smaller than those of control subjects. Bulbar function and ERD were negatively correlated in ALS patients. Motor function of the upper extremities did was uncorrelated with ERD. Conclusions ALS patients with worsened bulbar scales may show smaller ERD. Motor function of the upper extremities did was uncorrelated with ERD.

  9. Ultrafast Excited State Dynamics in Molecular Motors : Coupling of Motor Length to Medium Viscosity

    NARCIS (Netherlands)

    Conyard, Jamie; Stacko, Peter; Chen, Jiawen; McDonagh, Sophie; Hall, Christopher R.; Laptenok, Sergey P.; Browne, Wesley R.; Feringa, Ben L.; Meech, Stephen R.

    2017-01-01

    Photochemically driven molecular motors convert the energy of incident radiation to intramolecular rotational motion. The motor molecules considered here execute four step unidirectional rotational motion. This comprises a pair of successive light induced isomerizations to a metastable state

  10. Neuronal mechanisms of motor learning and motor memory consolidation in healthy old adults.

    Science.gov (United States)

    Berghuis, K M M; Veldman, M P; Solnik, S; Koch, G; Zijdewind, I; Hortobágyi, T

    2015-06-01

    It is controversial whether or not old adults are capable of learning new motor skills and consolidate the performance gains into motor memory in the offline period. The underlying neuronal mechanisms are equally unclear. We determined the magnitude of motor learning and motor memory consolidation in healthy old adults and examined if specific metrics of neuronal excitability measured by magnetic brain stimulation mediate the practice and retention effects. Eleven healthy old adults practiced a wrist extension-flexion visuomotor skill for 20 min (MP, 71.3 years), while a second group only watched the templates without movements (attentional control, AC, n = 11, 70.5 years). There was 40 % motor learning in MP but none in AC (interaction, p learn a new motor skill and consolidate the learned skill into motor memory, processes that are most likely mediated by disinhibitory mechanisms. These results are relevant for the increasing number of old adults who need to learn and relearn movements during motor rehabilitation.

  11. Modeling an electric motor in 1-D

    Science.gov (United States)

    Butler, Thomas G.

    1991-01-01

    Quite often the dynamicist will be faced with having an electric drive motor as a link in the elastic path of a structure such that the motor's characteristics must be taken into account to properly represent the dynamics of the primary structure. He does not want to model it so accurately that he could get detailed stress and displacements in the motor proper, but just sufficiently to represent its inertia loading and elastic behavior from its mounting bolts to its drive coupling. Described here is how the rotor and stator of such a motor can be adequately modeled as a colinear pair of beams.

  12. Electric motor predictive and preventive maintenance guide

    International Nuclear Information System (INIS)

    Oliver, J.A.

    1992-07-01

    Electric motor performance is vital to the reliable and efficient operation of power plants. The failure of one or more critical motors could cause lost capacity and excessive repair and maintenance cost. However, existing maintenance recommendations proposed by vendors for electric motors have sometimes encouraged many overly conservative maintenance practices. These practices have lead to excessive maintenance activities and costs which have provided no extra margin of operability. EPRI has sponsored RP2814-35 to develop a guide which provides power plants with information and guidance for establishing an effective maintenance program which will aid in preventing unexpected motor failures and assist in planning motor maintenance efforts. The guide includes a technical description which summarizes technical data relative to the four basic types of motors and their components in general use in power plants. The significant causes of motor failures are investigated and described in detail and methods to optimize service life and minimize maintenance cost through appropriate preventive maintenance and conditioning program are presented. This guide provides a foundation for an effective electric motor maintenance program and simplifies the selection of predictive and preventive maintenance tasks. Its use will enable maintenance personnel in nuclear and fossil plants to plan motor repairs during scheduled outages and avoid costly unexpected failures

  13. Premium Efficiency Motors And Market Penetration Policy

    Energy Technology Data Exchange (ETDEWEB)

    Benhaddadi, Mohamed; Olivier, Guy

    2010-09-15

    This paper illustrates the induced enormous energy saving potential, permitted by using high-efficiency motors. Furthermore, the most important barriers to larger high-efficiency motors utilization are identified, and some incentives recommendations are given to overcome identified impediments. The authors consider that there is a strong case to enhance incentives policies for larger market penetration. The US Energy Policy Act and the Canadian Energy Efficient Act have lead to North American leadership on motor efficiency implementation. North America is not on the leading edge for energy saving and conservation. Motor efficiency is an exception that should be at least maintained.

  14. Presentation of electric motor and motor control technology for electric vehicles and hybrid vehicles; Denki jidosha hybrid sha yo motor oyobi motor seigyo gijutsu no shokai

    Energy Technology Data Exchange (ETDEWEB)

    Matsudaira, N.; Masakik, R.; Tajima, F. [Hitachi, Ltd., Tokyo (Japan)

    1999-02-01

    The authors have developed a motor drive system for electric vehicles and hybrid vehicles. This system consists of a permanent magnet type synchronous motor, an inverter using insulated gate bipolar transistors (IGBTs) and a controller based on a single-chip microcomputer. To achieve a compact and light weight synchronous motor, an internal permanent magnet type rotor structure was designed. This paper presents motor control technology for electric vehicles, such as an optimization method of field weakening control and a new current control method. (author)

  15. Introduction to the permanent magnet motor market

    Energy Technology Data Exchange (ETDEWEB)

    Sawa, Toshihiro; Hamada, Kaneyuki [Yaskawa Electric Corp. (Japan)

    2000-07-01

    According to the Kyoto summit on global warming (COP3) in December 1997, the green-house gas emission level has to be reduced to 92-94% of the 1990 green-house gas level by the year 2014-2018. This would require conserving energy. An efficient means of achieving this voluntary goal is by employing high-efficiency drives, since motors consume 70% of all electricity for industrial use in Japan. As adjustable speed drives become popular, interior permanent magnet (IPM) motors, lately, have been recognized for high-efficiency performance. Due to the progress in permanent magnet technology combined with modern control methods, especially vector control with and without speed-sensors, the IPM motor is gaining in popularity. Compact size and high-efficiency performance is furthering the IPM motor as the preferred motor in many applications. This paper describes the principle and operation of IPM motors and compares its performance with that of an induction motor. Important features and practical control methods for IPM motors are presented. Various application examples highlighting the advantages of employing an IPM motor system are discussed. The applications include, but are not limited to, machine tools, fans, pumps, elevators, cranes, etc. (orig.)

  16. Linear Motor for Drive of Belt Conveyor

    Directory of Open Access Journals (Sweden)

    Milan Krasl

    2006-01-01

    Full Text Available This paper introduces a novel approach on the design of a linear motor for drive of belt conveyor (LMBC. The motor is a simple combination of asynchronous motor in plane. The electromagnetic forces is one of the most important parameters of electrical machines. This parameter is necessary for the checking of the design. This paper describes several variants: linear motor with slots in platens, slots in one half of platens and optimization of slots. The electromagnetic force can be found with the help of a Finite Elements Method – based program. For solution was used QuickField program.

  17. Parkinson's disease motor subtypes and mood.

    Science.gov (United States)

    Burn, David J; Landau, Sabine; Hindle, John V; Samuel, Michael; Wilson, Kenneth C; Hurt, Catherine S; Brown, Richard G

    2012-03-01

    Parkinson's disease is heterogeneous, both in terms of motor symptoms and mood. Identifying associations between phenotypic variants of motor and mood subtypes may provide clues to understand mechanisms underlying mood disorder and symptoms in Parkinson's disease. A total of 513 patients were assessed using the Hospital Anxiety and Depression Scale, and separately classified into anxious, depressed, and anxious-depressed mood classes based on latent class analysis of a semistructured interview. Motor subtypes assessed related to age-of-onset, rate of progression, presence of motor fluctuations, lateralization of motor symptoms, tremor dominance, and the presence of postural instability and gait symptoms and falls. The directions of observed associations tended to support previous findings with the exception of lateralization of symptoms, for which there were no consistent or significant results. Regression models examining a range of motor subtypes together indicated increased risk of anxiety in patients with younger age-of-onset and motor fluctuations. In contrast, depression was most strongly related to axial motor symptoms. Different risk factors were observed for depressed patients with and without anxiety, suggesting heterogeneity within Parkinson's disease depression. Such association data may suggest possible underlying common risk factors for motor subtype and mood. Combined with convergent evidence from other sources, possible mechanisms may include cholinergic system damage and white matter changes contributing to non-anxious depression in Parkinson's disease, while situational factors related to threat and unpredictability may contribute to the exacerbation and maintenance of anxiety in susceptible individuals. Copyright © 2011 Movement Disorder Society.

  18. Mechatronical Design Studies on Small Brushless Motors

    Directory of Open Access Journals (Sweden)

    W. Amrhein

    2003-01-01

    Full Text Available Brushless DC- and AC-permanent-magnet motors controlled by powerful micro-controller electronics have opened up a significant share of the small motor market in the last years. Based on the mechanical low cost construction of single-phase motor the paper presents electronic drive concepts to improve the performance and for special applications also the lifetime of brushless motors. The tangential and radial forces acting on the rotor are controlled by special phase current curves to reduce the torque ripple and to avoid expendable machinery parts like ball or sliding bearings.

  19. The motor theory of speech perception revisited.

    Science.gov (United States)

    Massaro, Dominic W; Chen, Trevor H

    2008-04-01

    Galantucci, Fowler, and Turvey (2006) have claimed that perceiving speech is perceiving gestures and that the motor system is recruited for perceiving speech. We make the counter argument that perceiving speech is not perceiving gestures, that the motor system is not recruitedfor perceiving speech, and that speech perception can be adequately described by a prototypical pattern recognition model, the fuzzy logical model of perception (FLMP). Empirical evidence taken as support for gesture and motor theory is reconsidered in more detail and in the framework of the FLMR Additional theoretical and logical arguments are made to challenge gesture and motor theory.

  20. The Model of Brushless Dc Motor Drive

    Directory of Open Access Journals (Sweden)

    Aurelijus Pitrėnas

    2013-05-01

    Full Text Available The research considered the operation, control, mathematical and simulation models of BLDC motor. A simplified idealized simulation model was designed and tested using Matlab Simulink software package. The simulation model uses Hall effect sensor signals for determining the rotor position. Simulation was done for Maxon, EC-4 pole 22 BL A series motor. The obtained model testing results deviate from the data supplied by the motor manufacturer by as little as 0.2–10.6%; consequently, the implemented model is suitable for BLDC motor control study and research.Article in Lithuanian

  1. Electronically commutated DC motor. Elektronisch kommutierter Gleichstrommotor

    Energy Technology Data Exchange (ETDEWEB)

    Gruenleitner, H; Schalk, K; Koegler, G

    1981-08-13

    The purpose of the invention is to create a controlled and electronically commutated DC motor, so that the braking current regulator does not act with the frequency motor current regulator, where an additional switch is not required to decouple the braking current transistor while running, and where the reference value of braking current need not be greater than the reference value of motor current. According to the invention, this problem is solved by a connection, by which, while running, the braking current regulator is interlocked out by means of the output signal of the motor current regulator. A cheap diode and the associated wiring are all that is required for the interlock.

  2. Analisa Perbandingan Efisiensi Motor Dc Kompon Pendek Dengan Motor Dc Kompon Panjang Akibat Penambahan Kutub

    OpenAIRE

    Sitompul, Fuad Rahim

    2015-01-01

    Motor listrik merupakan perangkat elekromagnetis yang mengubah energi listrik menjadi energi mekanik. Motor DC memerlukan tegangan searah pada kumparan medan untuk diubah menjadi energi mekanik. Energi mekanik ini digunakan sebagai penggerak peralatan listrik seperti pompa,penggerak kipas angin, lift dan lain-lain. Karena penggunaannya yang cukup luas maka kinerjanya harus baik. Kinerja suatu motor DC dikatakan baik jika efisiensi motor tersebut tinggi. Hal itu dapat dicapai dengan mengatur b...

  3. Fundamental motor skill proficiency is necessary for children's motor activity inclusion

    OpenAIRE

    Barela, José Angelo

    2013-01-01

    Motor development is influenced by many factors such as practice and appropriate instruction, provided by teachers, even in preschool and elementary school. The goal of this paper was to discuss the misconception that maturation underlies children's motor skill development and to show that physical education, even in early years of our school system, is critical to promote proficiency and enrolment of children's in later motor activities. Motor skill development, as a curricular focus, has be...

  4. Imaging the ocular motor nerves

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, Teresa [Department of Radiology, Leiden University Medical Center (Netherlands)], E-mail: T.A.Ferreira@lumc.nl; Verbist, Berit [Department of Radiology, Leiden University Medical Center (Netherlands)], E-mail: B.M.Verbist@lumc.nl; Buchem, Mark van [Department of Radiology, Leiden University Medical Center (Netherlands)], E-mail: M.A.van_Buchem@lumc.nl; Osch, Thijs van [C.J. Gorter for High-Field MRI, Department of Radiology, Leiden University Medical Center (Netherlands)], E-mail: M.J.P.van_Osch@lumc.nl; Webb, Andrew [C.J. Gorter for High-Field MRI, Department of Radiology, Leiden University Medical Center (Netherlands)], E-mail: A.Webb@lumc.nl

    2010-05-15

    The ocular motor nerves (OMNs) comprise the oculomotor, trochlear and the abducens nerves. According to their course, they are divided into four or five anatomic segments: intra-axial, cisternal, cavernous and intra-orbital and, for the abducens nerve, an additional interdural segment. Magnetic resonance imaging is the imaging method of choice in the evaluation of the normal and pathologic ocular motor nerves. CT still plays a limited but important role in the evaluation of the intraosseous portions at the skull base and bony foramina. We describe for each segment of these cranial nerves, the normal anatomy, the most appropriate image sequences and planes, their imaging appearance and pathologic conditions. Magnetic resonance imaging with high magnetic fields is a developing and promising technique. We describe our initial experience with a Phillips 7.0 T MRI scanner in the evaluation of the brainstem segments of the OMNs. As imaging becomes more refined, an understanding of the detailed anatomy is increasingly necessary, as the demand on radiology to diagnose smaller lesions also increases.

  5. Esophageal motor disorders: recent advances.

    Science.gov (United States)

    Dogan, Ibrahim; Mittal, Ravinder K

    2006-07-01

    The aim of this article is to highlight literature published during the last year in the context of previous knowledge. A number of novel techniques - high-resolution manometry, esophageal electrical impedance and intra-luminal ultrasound imaging - have improved our understanding of esophageal function in health and disease. Several studies address the function of longitudinal muscle layer of the esophagus in normal subjects and patients with motor disorders of the esophagus. Esophageal electrical impedance recordings reveal abnormal transit in patients with diffuse esophageal spasm, achalasia and patients with normal manometry. Loss of the mammalian Sprouty2 gene leads to enteric neuronal hyperplasia and esophageal achalasia. Several studies showed excellent long-term results of medical and surgical treatment of achalasia of the esophagus. For the first time, mechanisms of gastroesophageal reflux in critically ill mechanically ventilated patients are reported. Novel pharmacologic strategies in the treatment of reflux disease are highlighted. Several novel techniques, perfected during recent years, have improved our understanding of esophageal function and dysfunction. A number of important observations, reviewed here, provide important insight into the pathogenesis of esophageal motor disorders and treatment of gastroesophageal reflux disease.

  6. Bone and motor organ diseases

    International Nuclear Information System (INIS)

    Fujiwara, Saeko

    1992-01-01

    Osteosarcoma arising from X-ray radiation therapy has first been reported in the 1920s. The 1950-1965 ABCC-RERF Life Span Study using the sample population of approximately 76,000 persons have revealed no evidence of correlation between osteosarcoma and A-bomb radiation. There is a relative paucity of data supporting the statistical correlation between A-bomb radiation and osseous cancer. This paper deals with the correlation between A-bomb exposure and bone and motor organ diseases. In prenatally exposed children using the Nagasaki's sample (n=74) and the Hiroshima's sample (n=219), there was no difference in skeletal abnormalities between the exposed and control groups in both cities. In the ABCC-study using 264 A-bomb survivors, the incidence of osteoporosis was found to be high in women aged 50 years or older at the time of A-bombing who were exposed at 2,000 m or less from the hypocenter. The RERF Adult Health Study using approximately 14,000 persons have revealed no evidence of correlation between the incidence of lumbar vertebral bone fractures and radiation doses. There was no correlation between the prevalence of rheumatoid arthritis and distance from the hypocenter in A-bomb survivors. Continuing studies are expected to confirm the delayed effects of A-bomb radiation on the bone and motor organs with aging in A-bomb survivors. (N.K.)

  7. Automation of motor dexterity assessment.

    Science.gov (United States)

    Heyer, Patrick; Castrejon, Luis R; Orihuela-Espina, Felipe; Sucar, Luis Enrique

    2017-07-01

    Motor dexterity assessment is regularly performed in rehabilitation wards to establish patient status and automatization for such routinary task is sought. A system for automatizing the assessment of motor dexterity based on the Fugl-Meyer scale and with loose restrictions on sensing technologies is presented. The system consists of two main elements: 1) A data representation that abstracts the low level information obtained from a variety of sensors, into a highly separable low dimensionality encoding employing t-distributed Stochastic Neighbourhood Embedding, and, 2) central to this communication, a multi-label classifier that boosts classification rates by exploiting the fact that the classes corresponding to the individual exercises are naturally organized as a network. Depending on the targeted therapeutic movement class labels i.e. exercises scores, are highly correlated-patients who perform well in one, tends to perform well in related exercises-; and critically no node can be used as proxy of others - an exercise does not encode the information of other exercises. Over data from a cohort of 20 patients, the novel classifier outperforms classical Naive Bayes, random forest and variants of support vector machines (ANOVA: p home-based virtual rehabilitation and telerehabilitation alternatives.

  8. 36 CFR 292.45 - Use of motorized and non-motorized rivercraft.

    Science.gov (United States)

    2010-07-01

    ... practicable, conflicts between motorized and non-motorized rivercraft users and between both types of...-motorized rivercraft may be permitted subject to restrictions on size, type of craft, numbers, duration... Service where such activity may be permitted subject to restrictions on size, type of craft, numbers...

  9. DEVELOPMENT OF FINE MOTOR COORDINATION AND VISUAL-MOTOR INTEGRATION IN PRESCHOOL CHILDREN

    Directory of Open Access Journals (Sweden)

    Haris MEMISEVIC

    2013-03-01

    Full Text Available Fine motor skills are prerequisite for many everyday activities and they are a good predictor of a child's later academic outcome. The goal of the present study was to assess the effects of age on the development of fine motor coordination and visual-motor integration in preschool children. The sample for this study consisted of 276 preschool children from Canton Sara­jevo, Bosnia and Herzegovina. We assessed children's motor skills with Beery Visual Motor Integration Test and Lafayette Pegboard Test. Data were analyzed with one-way ANOVA, followed by planned com­parisons between the age groups. We also performed a regression analysis to assess the influence of age and motor coordination on visual-motor integration. The results showed that age has a great effect on the development of fine motor skills. Furthermore, the results indicated that there are possible sensitive periods at preschool age in which the development of fine motor skills is accelerated. Early intervention specialists should make a thorough evaluations of fine motor skills in preschool children and make motor (rehabilitation programs for children at risk of fine motor delays.

  10. 75 FR 67770 - General Motors Company, Formerly Known as General Motors Corporation, Orion Assembly Plant...

    Science.gov (United States)

    2010-11-03

    ..., Formerly Known as General Motors Corporation, Orion Assembly Plant, Including On-Site Leased Workers From Aerotek Automotive, Ryder and Premier Manufacturing Support Services, Lake Orion, MI; Amended... of General Motors Company, formerly known as General Motors Corporation, Orion Assembly Plant, Lake...

  11. Repetitive Transcranial Magnetic Stimulation to the Primary Motor Cortex Interferes with Motor Learning by Observing

    Science.gov (United States)

    Brown, Liana E.; Wilson, Elizabeth T.; Gribble, Paul L.

    2009-01-01

    Neural representations of novel motor skills can be acquired through visual observation. We used repetitive transcranial magnetic stimulation (rTMS) to test the idea that this "motor learning by observing" is based on engagement of neural processes for learning in the primary motor cortex (M1). Human subjects who observed another person learning…

  12. Enhanced Multisensory Integration and Motor Reactivation after Active Motor Learning of Audiovisual Associations

    Science.gov (United States)

    Butler, Andrew J.; James, Thomas W.; James, Karin Harman

    2011-01-01

    Everyday experience affords us many opportunities to learn about objects through multiple senses using physical interaction. Previous work has shown that active motor learning of unisensory items enhances memory and leads to the involvement of motor systems during subsequent perception. However, the impact of active motor learning on subsequent…

  13. Cerebellar motor learning versus cerebellar motor timing: the climbing fibre story

    Science.gov (United States)

    Llinás, Rodolfo R

    2011-01-01

    Abstract Theories concerning the role of the climbing fibre system in motor learning, as opposed to those addressing the olivocerebellar system in the organization of motor timing, are briefly contrasted. The electrophysiological basis for the motor timing hypothesis in relation to the olivocerebellar system is treated in detail. PMID:21486816

  14. 77 FR 4396 - Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; Toyota

    Science.gov (United States)

    2012-01-27

    ... From the Federal Motor Vehicle Motor Theft Prevention Standard; Toyota AGENCY: National Highway Traffic... exemption. SUMMARY: This document grants in full the petition of Toyota Motor North America, Inc's., (Toyota.... SUPPLEMENTARY INFORMATION: In a petition dated September 30, 2011, Toyota requested an exemption from the parts...

  15. 78 FR 3081 - Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; Toyota

    Science.gov (United States)

    2013-01-15

    ... From the Federal Motor Vehicle Motor Theft Prevention Standard; Toyota AGENCY: National Highway Traffic.... SUMMARY: This document grants in full Toyota Motor North America, Inc.'s (Toyota) petition for an... a petition dated October 16, 2012, Toyota requested an exemption from the parts-marking requirements...

  16. 76 FR 12221 - Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; Toyota

    Science.gov (United States)

    2011-03-04

    ... From the Federal Motor Vehicle Motor Theft Prevention Standard; Toyota AGENCY: National Highway Traffic.... SUMMARY: This document grants in full the petition of Toyota Motor North America, Inc's., (Toyota... INFORMATION: In a petition dated January 24, 2011, Toyota requested an exemption from the parts-marking...

  17. A Motor-Skills Programme to Enhance Visual Motor Integration of Selected Pre-School Learners

    Science.gov (United States)

    Africa, Eileen K.; van Deventer, Karel J.

    2017-01-01

    Pre-schoolers are in a window period for motor skill development. Visual-motor integration (VMI) is the foundation for academic and sport skills. Therefore, it must develop before formal schooling. This study attempted to improve VMI skills. VMI skills were measured with the "Beery-Buktenica developmental test of visual-motor integration 6th…

  18. Chemical and mechanical efficiencies of molecular motors and implications for motor mechanisms

    International Nuclear Information System (INIS)

    Wang Hongyun

    2005-01-01

    Molecular motors operate in an environment dominated by viscous friction and thermal fluctuations. The chemical reaction in a motor may produce an active force at the reaction site to directly move the motor forward. Alternatively a molecular motor may generate a unidirectional motion by rectifying thermal fluctuations using free energy barriers established in the chemical reaction. The reaction cycle has many occupancy states, each having a different effect on the motor motion. The average effect of the chemical reaction on the motor motion can be characterized by the motor potential profile. The biggest advantage of studying the motor potential profile is that it can be reconstructed from the time series of motor positions measured in single-molecule experiments. In this paper, we use the motor potential profile to express the Stokes efficiency as the product of the chemical efficiency and the mechanical efficiency. We show that both the chemical and mechanical efficiencies are bounded by 100% and, thus, are properly defined efficiencies. We discuss implications of high efficiencies for motor mechanisms: a mechanical efficiency close to 100% implies that the motor potential profile is close to a constant slope; a chemical efficiency close to 100% implies that (i) the chemical transitions are not slower than the mechanical motion and (ii) the equilibrium constant of each chemical transition is close to one

  19. 77 FR 29752 - Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; Jaguar...

    Science.gov (United States)

    2012-05-18

    ... From the Federal Motor Vehicle Motor Theft Prevention Standard; Jaguar Land Rover AGENCY: National... part 543, Exemption from the Theft Prevention Standard. This petition is granted, because the agency... be as effective in reducing and deterring motor vehicle theft as compliance with the parts-marking...

  20. 76 FR 12220 - Petition for Exemption From the Federal Motor Vehicle Motor Theft Prevention Standard; Jaguar...

    Science.gov (United States)

    2011-03-04

    ... From the Federal Motor Vehicle Motor Theft Prevention Standard; Jaguar Land Rover AGENCY: National... 543, Exemption from the Theft Prevention Standard. This petition is granted because the agency has... effective in reducing and deterring motor vehicle theft as compliance with the parts-marking requirements of...

  1. 50 CFR 35.5 - Commercial enterprises, roads, motor vehicles, motorized equipment, motorboats, aircraft...

    Science.gov (United States)

    2010-10-01

    ... 50 Wildlife and Fisheries 6 2010-10-01 2010-10-01 false Commercial enterprises, roads, motor... Rules § 35.5 Commercial enterprises, roads, motor vehicles, motorized equipment, motorboats, aircraft... private rights, there shall be no commercial enterprise and no permanent road within a wilderness unit...

  2. Brief Assessment of Motor Function: Content Validity and Reliability of the Upper Extremity Gross Motor Scale

    Science.gov (United States)

    Cintas, Holly Lea; Parks, Rebecca; Don, Sarah; Gerber, Lynn

    2011-01-01

    Content validity and reliability of the Brief Assessment of Motor Function (BAMF) Upper Extremity Gross Motor Scale (UEGMS) were evaluated in this prospective, descriptive study. The UEGMS is one of five BAMF ordinal scales designed for quick documentation of gross, fine, and oral motor skill levels. Designed to be independent of age and…

  3. No Role for Motor Affordances in Visual Working Memory

    Science.gov (United States)

    Pecher, Diane

    2013-01-01

    Motor affordances have been shown to play a role in visual object identification and categorization. The present study explored whether working memory is likewise supported by motor affordances. Use of motor affordances should be disrupted by motor interference, and this effect should be larger for objects that have motor affordances than for…

  4. Mountain Plains Learning Experience Guide: Electric Motor Repair.

    Science.gov (United States)

    Ziller, T.

    This Electric Motor Repair Course is designed to provide the student with practical information for winding, repairing, and troubleshooting alternating current and direct current motors, and controllers. The course is comprised of eight units: (1) Electric Motor Fundamentals, (2) Rewinding, (3) Split-phase Induction Motors, (4) Capacitor Motors,…

  5. 41 CFR 109-38.5103 - Motor vehicle utilization standards.

    Science.gov (United States)

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false Motor vehicle... AVIATION, TRANSPORTATION, AND MOTOR VEHICLES 38-MOTOR EQUIPMENT MANAGEMENT 38.51-Utilization of Motor Equipment § 109-38.5103 Motor vehicle utilization standards. (a) The following average utilization standards...

  6. 49 CFR 574.9 - Requirements for motor vehicle dealers.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Requirements for motor vehicle dealers. 574.9... RECORDKEEPING § 574.9 Requirements for motor vehicle dealers. (a) Each motor vehicle dealer who sells a used motor vehicle for purposes other than resale, who leases a motor vehicle for more than 60 days, that is...

  7. Induction motor for superconducting synchronous/asynchronous motor

    International Nuclear Information System (INIS)

    Litz, D.C.; Haller, H.E. III.

    1975-01-01

    An induction motor structure for use on the outside of a superconducting rotor comprising a cylindrical shell of solid and laminated, magnetic iron with squirrel cage windings embedded in the outer circumference of said shell is described. The sections of the shell between the superconducting windings of the rotor are solid magnetic iron. The sections of the shell over the superconducting windings are made of laminations of magnetic iron. These laminations are parallel to the axis of the machine and are divided in halves with the laminations in each half oriented in diagonal opposition so that the intersection of the laminations forms a V. This structure presents a relatively high reluctance to leakage flux from the superconducting windings in the synchronous operating mode, while presenting a low reluctance path to the stator flux during asynchronous operation

  8. 75 FR 26794 - New United Motor Manufacturing, Inc., Formerly a Joint Venture of General Motors Corporation and...

    Science.gov (United States)

    2010-05-12

    ... Manufacturing, Inc., Formerly a Joint Venture of General Motors Corporation and Toyota Motor Corporation... United Motor Manufacturing, Inc., formerly a joint venture of General Motors Corporation and Toyota Motor... reviewed the certification for workers of the subject firm. The workers assemble the Toyota Corolla and the...

  9. 76 FR 10396 - New United Motor Manufacturing, Inc., Formerly a Joint Venture of General Motors Corporation and...

    Science.gov (United States)

    2011-02-24

    ... Manufacturing, Inc., Formerly a Joint Venture of General Motors Corporation and Toyota Motor Corporation, Including On- Site Leased Workers From Corestaff, ABM Janitorial, Toyota Engineering and Manufacturing North... Motor Manufacturing, Inc., formerly a joint venture of General Motors Corporation and Toyota Motor...

  10. 33 CFR 127.311 - Motor vehicles.

    Science.gov (United States)

    2010-07-01

    ...) WATERFRONT FACILITIES WATERFRONT FACILITIES HANDLING LIQUEFIED NATURAL GAS AND LIQUEFIED HAZARDOUS GAS Waterfront Facilities Handling Liquefied Natural Gas Operations § 127.311 Motor vehicles. (a) The operator... storage tank or loading flange. (b) During transfer operations, no person may— (1) Stop or park a motor...

  11. Analysing Simple Electric Motors in the Classroom

    Science.gov (United States)

    Yap, Jeff; MacIsaac, Dan

    2006-01-01

    Electromagnetic phenomena and devices such as motors are typically unfamiliar to both teachers and students. To better visualize and illustrate the abstract concepts (such as magnetic fields) underlying electricity and magnetism, we suggest that students construct and analyse the operation of a simply constructed Johnson electric motor. In this…

  12. Oscillation control system for electric motor drive

    Science.gov (United States)

    Slicker, J.M.; Sereshteh, A.

    1988-08-30

    A feedback system for controlling mechanical oscillations in the torsionally complaint drive train of an electric or other vehicle. Motor speed is converted in a processor to estimate state signals in which a plant model which are used to electronically modify the torque commands applied to the motor. 5 figs.

  13. Motor Control Research Requires Nonlinear Dynamics

    Science.gov (United States)

    Guastello, Stephen J.

    2006-01-01

    The author comments on the original article "The Cinderella of psychology: The neglect of motor control in the science of mental life and behavior," by D. A. Rosenbaum. Rosenbaum draws attention to the study of motor control and evaluates seven possible explanations for why the topic has been relatively neglected. The point of this comment is that…

  14. Microcomputer-based stepping-motor controller

    International Nuclear Information System (INIS)

    Johnson, K.

    1983-04-01

    A microcomputer-controlled stepping motor is described. A Motorola MC68701 microcomputer unit is interfaced to a Cybernetic CY500 stored-program controller that outputs through Motorola input/output isolation modules to the stepping motor. A complex multifunction controller with enhanced capabilities is thus available with a minimum number of parts

  15. Vital Signs-Motor Vehicle Crash Injuries

    Centers for Disease Control (CDC) Podcasts

    2014-10-07

    This podcast is based on the October 2014 CDC Vital Signs report. Motor vehicle crashes are costly and preventable. Learn what can be done to help prevent motor vehicle injuries.  Created: 10/7/2014 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 10/7/2014.

  16. Motor Vehicle Crash Injuries PSA (:60)

    Centers for Disease Control (CDC) Podcasts

    2014-10-07

    This 60 second Public Service Announcement is based on the October 2014 CDC Vital Signs report. Motor vehicle crashes are costly and preventable. Learn what can be done to help prevent motor vehicle injuries.  Created: 10/7/2014 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 10/7/2014.

  17. Dual wound dc brush motor gearhead

    Science.gov (United States)

    Henson, Barrie W.

    1986-01-01

    The design requirements, the design, development tests and problems, the qualification and life test and the findings of the strip examination of a dual wound DC brushed motor gearhead are described. It is the only space qualified dual wound dc brushed motor gearhead in Europe.

  18. Gravity Compensation Technique Uses Small dc Motor

    Science.gov (United States)

    Hollow, Richard

    1988-01-01

    Small dc servomotor powered by simple constant-current source and with suitable gearing used to cancel effect of gravity upon load. Lead-screw positioning system has load counterbalanced by small supplementary motor powered by constant current source. Motor lighter and more compact alternative to counterbalance. Used in variety of mechanical systems where load positioned or accelerated in vertical plane.

  19. Computerized Torque Control for Large dc Motors

    Science.gov (United States)

    Willett, Richard M.; Carroll, Michael J.; Geiger, Ronald V.

    1987-01-01

    Speed and torque ranges in generator mode extended. System of shunt resistors, electronic switches, and pulse-width modulation controls torque exerted by large, three-phase, electronically commutated dc motor. Particularly useful for motor operating in generator mode because it extends operating range to low torque and high speed.

  20. Advanced dc-Traction-Motor Control System

    Science.gov (United States)

    Vittone, O.

    1985-01-01

    Motor-control concept for battery-powered vehicles includes stateof-the-art power-transistor switching and separate excitation of motor windings in traction and regenerative braking. Switching transistors and other components of power-conditioning subsystem operate under control of computer that coordinates traction, braking, and protective functions.

  1. Linear motor with contactless energy transfer

    NARCIS (Netherlands)

    2014-01-01

    An integrated electromagnetic energy conversions device is provided that includes a synchronous or brushless linear (SoBL) motor, and a transformer, where the transformer is integrated electromagnetically and topologically with the SoBL motor, where an electromagnetic field orientation of the

  2. Motor subtype changes in early Parkinson's disease.

    Science.gov (United States)

    Eisinger, Robert S; Hess, Christopher W; Martinez-Ramirez, Daniel; Almeida, Leonardo; Foote, Kelly D; Okun, Michael S; Gunduz, Aysegul

    2017-10-01

    Distinct motor subtypes of Parkinson's disease (PD) have been described through both clinical observation and through data-driven approaches. However, the extent to which motor subtypes change during disease progression remains unknown. Our objective was to determine motor subtypes of PD using an unsupervised clustering methodology and evaluate subtype changes with disease duration. The Parkinson's Progression Markers Initiative database of 423 newly diagnosed PD patients was utilized to retrospectively identify unique motor subtypes through a data-driven, hierarchical correlational clustering approach. For each patient, we assigned a subtype to each motor assessment at each follow-up visit (time points) and by using published criteria. We examined changes in PD subtype with disease duration using both qualitative and quantitative methods. Five distinct motor subtypes were identified based on the motor assessment items and these included: Tremor Dominant (TD), Axial Dominant, Appendicular Dominant, Rigidity Dominant, and Postural and Instability Gait Disorder Dominant. About half of the patients had consistent subtypes at all time points. Most patients met criteria for TD subtype soon after diagnosis. For patients with inconsistent subtypes, there was an overall trend to shift away from a TD phenotype with disease duration, as shown by chi-squared test, p motor subtypes in PD can shift with increasing disease duration. Shifting subtypes is a factor that should be accounted for in clinical practice or in clinical trials. Copyright © 2017 Elsevier Ltd. All rights reserved.

  3. Assessment of Motor Units in Neuromuscular Disease.

    Science.gov (United States)

    Henderson, Robert D; McCombe, Pamela A

    2017-01-01

    The motor unit comprises the anterior horn cell, its axon, and the muscle fibers that it innervates. Although the true number of motor units is unknown, the number of motor units appears to vary greatly between different muscles and between different individuals. Assessment of the number and function of motor units is needed in diseases of the anterior horn cell and other motor nerve disorders. Amyotrophic lateral sclerosis is the most important disease of anterior horn cells. The need for an effective biomarker for assessing disease progression and for use in clinical trials in amyotrophic lateral sclerosis has stimulated the study of methods to measure the number of motor units. Since 1970 a number of different methods, including the incremental, F-wave, multipoint, and statistical methods, have been developed but none has achieved widespread applicability. Two methods (MUNIX and the multipoint incremental method) are in current use across multiple centres and are discussed in detail in this review, together with other recently published methods. Imaging with magnetic resonance and ultrasound is increasingly being applied to this area. Motor unit number estimates have also been applied to other neuromuscular diseases such as spinal muscular atrophy, compression neuropathies, and prior poliomyelitis. The need for an objective measure for the assessment of motor units remains tantalizingly close but unfulfilled in 2016.

  4. Motor automaticity in Parkinson’s disease

    Science.gov (United States)

    Wu, Tao; Hallett, Mark; Chan, Piu

    2017-01-01

    Bradykinesia is the most important feature contributing to motor difficulties in Parkinson’s disease (PD). However, the pathophysiology underlying bradykinesia is not fully understood. One important aspect is that PD patients have difficulty in performing learned motor skills automatically, but this problem has been generally overlooked. Here we review motor automaticity associated motor deficits in PD, such as reduced arm swing, decreased stride length, freezing of gait, micrographia and reduced facial expression. Recent neuroimaging studies have revealed some neural mechanisms underlying impaired motor automaticity in PD, including less efficient neural coding of movement, failure to shift automated motor skills to the sensorimotor striatum, instability of the automatic mode within the striatum, and use of attentional control and/or compensatory efforts to execute movements usually performed automatically in healthy people. PD patients lose previously acquired automatic skills due to their impaired sensorimotor striatum, and have difficulty in acquiring new automatic skills or restoring lost motor skills. More investigations on the pathophysiology of motor automaticity, the effect of L-dopa or surgical treatments on automaticity, and the potential role of using measures of automaticity in early diagnosis of PD would be valuable. PMID:26102020

  5. Simulation of linear Switched Reluctance Motor drives

    OpenAIRE

    Garcia Amoros, Jordi; Blanqué Molina, Balduino; Andrada Gascón, Pedro

    2011-01-01

    This paper presents a simulation model of linear switched reluctance motor drives. A Matlab-Simulink environment coupled with finite element analysis is used to perform the simulations. Experimental and simulation results for a double sided linear switched motor drive prototype are reported and compared to verify the simulation model.

  6. A linear motor as seismic horizontal vibrator

    NARCIS (Netherlands)

    Drijkoningen, G.; Veltman, A.; Hendrix, W.H.A.; Brouwer, J.; Hemstede, A.

    2006-01-01

    In this paper we propose to use the concept of linear synchronous motors to act as a seismic shear-wave vibratory source. We show that a linear motor, even with a design that is not focussed on application of seismic surveying, gives seismic records that are convincing and comparable with an

  7. Neuromodulation of vertebrate motor neuron membrane properties

    DEFF Research Database (Denmark)

    Hultborn, Hans; Kiehn, Ole

    1992-01-01

    The short-term function of motor neurons is to integrate synaptic inputs converging onto the somato-dendritic membrane and to transform the net synaptic drive into spike trains. A set of voltage-gated ion channels determines the electro-responsiveness and thereby the motor neuron's input-output f...

  8. Speed controller for an alternating - current motor

    International Nuclear Information System (INIS)

    Bolie, V.W.

    1984-01-01

    A controller for a multi-phase ac motor that is subject to a large inertial load, e.g. an induction motor driving a heavy spinning rotor of a neutron chopper that must be rotated in phase-locked synchronism with a reference pulse train that is representative of an ac power supply signal Esub(L) having a meandering line frequency, includes a sensor which provides a feedback pulse train representative of the actual speed of the motor which is compared (by counting clock pulses between feedback pulses) with a reference clock signal in a computing unit to provide a motor control signal Esub(c). The motor control signal is a weighted linear sum of a speed error signal, a phase error signal, and a drift error signal, the magnitudes of which are recalculated and updated with each revolution of the motor shaft. The speed error signal is constant for large speed errors but highly sensitive to small speed errors. The stator windings of the motor are driven by variable-frequency power amplifiers which are controlled by the motor control signal Esub(c) via PROMs which store digital representations of sine and cosine waveforms in quadrature. (author)

  9. Air pollution from motor vehicle emissions

    International Nuclear Information System (INIS)

    Petrushevska, Ljubica

    1996-01-01

    This paper presents some aspects of air pollution from motor vehicle emissions as: characteristic primary and secondary pollutants, dependence of the motor vehicle emission from the engine type; the relationship of typical engine emission and performance to air-fuel ratio, transport of pollutants from mobile sources of emissions, as well as some world experiences in the control approaches for exhaust emissions. (author)

  10. Validating the Rett Syndrome Gross Motor Scale.

    Directory of Open Access Journals (Sweden)

    Jenny Downs

    Full Text Available Rett syndrome is a pervasive neurodevelopmental disorder associated with a pathogenic mutation on the MECP2 gene. Impaired movement is a fundamental component and the Rett Syndrome Gross Motor Scale was developed to measure gross motor abilities in this population. The current study investigated the validity and reliability of the Rett Syndrome Gross Motor Scale. Video data showing gross motor abilities supplemented with parent report data was collected for 255 girls and women registered with the Australian Rett Syndrome Database, and the factor structure and relationships between motor scores, age and genotype were investigated. Clinical assessment scores for 38 girls and women with Rett syndrome who attended the Danish Center for Rett Syndrome were used to assess consistency of measurement. Principal components analysis enabled the calculation of three factor scores: Sitting, Standing and Walking, and Challenge. Motor scores were poorer with increasing age and those with the p.Arg133Cys, p.Arg294* or p.Arg306Cys mutation achieved higher scores than those with a large deletion. The repeatability of clinical assessment was excellent (intraclass correlation coefficient for total score 0.99, 95% CI 0.93-0.98. The standard error of measurement for the total score was 2 points and we would be 95% confident that a change 4 points in the 45-point scale would be greater than within-subject measurement error. The Rett Syndrome Gross Motor Scale could be an appropriate measure of gross motor skills in clinical practice and clinical trials.

  11. A Novel Approach to Diagnosing Motor Skills

    Science.gov (United States)

    Aguirre, Aitor; Lozano-Rodero, Alberto; Matey, Luis M.; Villamañe, Mikel; Ferrero, Begoña

    2014-01-01

    The combination of virtual reality interactive systems and educational technologies have been used in the training of procedural tasks, but there is a lack of research with regard to providing specific assistance for acquiring motor skills. In this paper we present a novel approach to evaluating motor skills with an interactive intelligent…

  12. Lateralization of cortical negative motor areas.

    Science.gov (United States)

    Borggraefe, Ingo; Catarino, Claudia B; Rémi, Jan; Vollmar, Christian; Peraud, Aurelia; Winkler, Peter A; Noachtar, Soheyl

    2016-10-01

    The lateral and mesial aspects of the central and frontal cortex were studied by direct electrical stimulation of the cortex in epilepsy surgery candidates in order to determine the localization of unilateral and bilateral negative motor responses. Results of electrical cortical stimulation were examined in epilepsy surgery candidates in whom invasive electrodes were implanted. The exact localization of subdural electrodes was defined by fusion of 3-dimensional reconstructed MRI and CT images in 13 patients and by analysis of plane skull X-rays and intraoperative visual localization of the electrodes in another 7 patients. Results of electrical stimulation of the cortex were evaluated in a total of 128 patients in whom invasive electrodes were implanted for planning resective epilepsy surgery. Twenty patients, in whom negative motor responses were obtained, were included in the study. Bilateral upper limb negative motor responses were more often elicited from stimulation of the mesial frontal cortex whereas stimulation of the lateral central cortex leads to contralateral upper limb negative motor responses (pfrontal gyrus whereas contralateral negative motor responses localized predominantly in the anterior part of the precentral gyrus (pgyrus and the mesial fronto-central cortex showing functional differences with regard to unilateral and bilateral upper limb representation. The lateral fronto-central negative motor area serves predominantly contralateral upper limb motor control whereas the mesial frontal negative motor area represents bilateral upper limb movement control. Copyright © 2016 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.

  13. Improved motor sequence retention by motionless listening.

    Science.gov (United States)

    Lahav, Amir; Katz, Tal; Chess, Roxanne; Saltzman, Elliot

    2013-05-01

    This study examined the effect of listening to a newly learned musical piece on subsequent motor retention of the piece. Thirty-six non-musicians were trained to play an unfamiliar melody on a piano keyboard. Next, they were randomly assigned to participate in three follow-up listening sessions over 1 week. Subjects who, during their listening sessions, listened to the same initial piece showed significant improvements in motor memory and retention of the piece despite the absence of physical practice. These improvements included increased pitch accuracy, time accuracy, and dynamic intensity of key pressing. Similar improvements, though to a lesser degree, were observed in subjects who, during their listening sessions, were distracted by another task. Control subjects, who after learning the piece had listened to nonmusical sounds, showed impaired motoric retention of the piece at 1 week from the initial acquisition day. These results imply that motor sequences can be established in motor memory without direct access to motor-related information. In addition, the study revealed that the listening-induced improvements did not generalize to the learning of a new musical piece composed of the same notes as the initial piece learned, limiting the effects to musical motor sequences that are already part of the individual's motor repertoire.

  14. Tidal dynamics in the sand motor lagoon

    NARCIS (Netherlands)

    De Vries, S.; Radermacher, M.; De Schipper, M.A.; Stive, M.J.F.

    2015-01-01

    The Sand Motor is a mega-nourishment characterized by a very large sand volume of around 20 million m3 placed along the Dutch coast. The Sand Motor is a pilot project to evaluate the performance of an alternative nourishment strategy with respect to different functions of the coastal system. Within

  15. 48 CFR 908.7101 - Motor vehicles.

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Motor vehicles. 908.7101 Section 908.7101 Federal Acquisition Regulations System DEPARTMENT OF ENERGY COMPETITION ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES AND SERVICES Acquisition of Special Items 908.7101 Motor vehicles. ...

  16. Timing and motor control in drumming

    DEFF Research Database (Denmark)

    Dahl, Sofia; Grossbach, Michael; Altenmüller, Eckart

    the stick movement becomes increasingly difficult, sometimes resulting in irregularities in timing and/or striking force. Timing irregularities can also be a revealing sign of motor control problems, such as focal dystonia (Jabusch, Vauth & Altenmüller, 2004). The "breakdown" in motor control can therefore...

  17. Motor Intentionality and the Case of Schneider

    DEFF Research Database (Denmark)

    Jensen, Rasmus Thybo

    2009-01-01

    I argue that Merleau-Ponty’s use of the case of Schneider in his arguments for the existence of non-conconceptual and non-representational motor intentionality contains a problematic methodological ambiguity. Motor intentionality is both to be revealed by its perspicuous preservation and by its...

  18. Design methods in solid rocket motors

    Energy Technology Data Exchange (ETDEWEB)

    1987-03-01

    A compilation of lectures summarizing the current state-of-the-art in designing solid rocket motors and and their components is presented. The experience of several countries in the use of new technologies and methods is represented. Specific sessions address propellant grains, cases, nozzles, internal thermal insulation, and the general optimization of solid rocket motor designs.

  19. Permanent magnet motor technology design and applications

    CERN Document Server

    Gieras, Jacek F

    2009-01-01

    Demonstrates the construction of permanent magnet (PM) motor drives and supplies ready-to-implement solutions to common roadblocks along the way. This book also supplies fundamental equations and calculations for determining and evaluating system performance, efficiency, reliability, and cost. It explores modern computer-aided design of PM motors.

  20. Validating the Rett Syndrome Gross Motor Scale.

    Science.gov (United States)

    Downs, Jenny; Stahlhut, Michelle; Wong, Kingsley; Syhler, Birgit; Bisgaard, Anne-Marie; Jacoby, Peter; Leonard, Helen

    2016-01-01

    Rett syndrome is a pervasive neurodevelopmental disorder associated with a pathogenic mutation on the MECP2 gene. Impaired movement is a fundamental component and the Rett Syndrome Gross Motor Scale was developed to measure gross motor abilities in this population. The current study investigated the validity and reliability of the Rett Syndrome Gross Motor Scale. Video data showing gross motor abilities supplemented with parent report data was collected for 255 girls and women registered with the Australian Rett Syndrome Database, and the factor structure and relationships between motor scores, age and genotype were investigated. Clinical assessment scores for 38 girls and women with Rett syndrome who attended the Danish Center for Rett Syndrome were used to assess consistency of measurement. Principal components analysis enabled the calculation of three factor scores: Sitting, Standing and Walking, and Challenge. Motor scores were poorer with increasing age and those with the p.Arg133Cys, p.Arg294* or p.Arg306Cys mutation achieved higher scores than those with a large deletion. The repeatability of clinical assessment was excellent (intraclass correlation coefficient for total score 0.99, 95% CI 0.93-0.98). The standard error of measurement for the total score was 2 points and we would be 95% confident that a change 4 points in the 45-point scale would be greater than within-subject measurement error. The Rett Syndrome Gross Motor Scale could be an appropriate measure of gross motor skills in clinical practice and clinical trials.

  1. DC motors and servo-motors controlled by Raspberry Pi 2B

    Directory of Open Access Journals (Sweden)

    Šustek Michal

    2017-01-01

    Full Text Available The expanding capabilities of today’s microcontrollers and other devices lead to an increased utilization of these technologies in diverse fields. The automation and issue of remote control of moving objects belong to these fields. In this project, a microcontroller Raspberry Pi 2B was chosen for controlling DC motors and servo-motors. This paper provides basic insight into issue of controlling DC motors and servo-motors, connection between Raspberry and other components on breadboard and programming syntaxes for controlling motors in Python programming language.

  2. Motor Skill Competence and Perceived Motor Competence: Which Best Predicts Physical Activity among Girls?

    OpenAIRE

    Khodaverdi, Zeinab; Bahram, Abbas; Khalaji, Hassan; Kazemnejad, Anoshirvan

    2013-01-01

    Abstract Background The main purpose of this study was to determine which correlate, perceived motor competence or motor skill competence, best predicts girls? physical activity behavior. Methods A sample of 352 girls (mean age=8.7, SD=0.3 yr) participated in this study. To assess motor skill competence and perceived motor competence, each child completed the Test of Gross Motor Development-2 and Physical Ability sub-scale of Marsh?s Self-Description Questionnaire. Children?s physical activit...

  3. Tongue motor training support system.

    Science.gov (United States)

    Sasaki, Makoto; Onishi, Kohei; Nakayama, Atsushi; Kamata, Katsuhiro; Stefanov, Dimitar; Yamaguchi, Masaki

    2014-01-01

    In this paper, we introduce a new tongue-training system that can be used for improvement of the tongue's range of motion and muscle strength after dysphagia. The training process is organized in game-like manner. Initially, we analyzed surface electromyography (EMG) signals of the suprahyoid muscles of five subjects during tongue-training motions. This test revealed that four types tongue training motions and a swallowing motion could be classified with 93.5% accuracy. Recognized EMG signals during tongue motions were designed to allow control of a mouse cursor via intentional tongue motions. Results demonstrated that simple PC games could be played by tongue motions, achieving in this way efficient, enjoyable and pleasant tongue training. Using the proposed method, dysphagia patients can choose games that suit their preferences and/or state of mind. It is expected that the proposed system will be an efficient tool for long-term tongue motor training and maintaining patients' motivation.

  4. Integration of motors and drives

    Energy Technology Data Exchange (ETDEWEB)

    Jackson, D.C. [Brook Hansen (United Kingdom)

    2000-07-01

    This paper examines the benefits of adopting a second-generation integrated motor and inverter. Removing the barriers to ensure that variable speed drives are more readily applied results in on-going cost savings to the user through energy savings plus process control benefits. In addition, the use of an integrated product instead of two separate components results in cost and time-savings to the installer. The simplification of integration, by transferring the guarantees of performance in efficiency, torque overload and stiffness, speed accuracy, noise and EMC compliance, allows optimisation by the design team to be realised by users and ease of application since the primary design team guarantees the product performance. The introduction of second generation compact product assists user conversion from present inefficient mechanical solutions. This technology is currently applicable in power ratings below 22 kW, which includes the vast majority of practical applications. (orig.)

  5. PFP supply fan motor starters

    International Nuclear Information System (INIS)

    Keck, R.D.

    1995-01-01

    The Plutonium Finishing Plant (PFP) is currently stabilizing about 25 kg of Pu sludge; upon completion of this task, PFP will be maintained in a safe standby condition to await decision from the PFP NEPA review. It can take about 10 years to initiate and complete terminal cleanout after this; the facility will then be decommissioned and decontaminated. The 234-5Z ventilation system must continue to operate until terminal cleanout. Part of the ventilation system is the seismic fan shutdown system which shuts down the ventilation supply fans in case of strong earthquake. This document presents criteria for installing solid state, reduced voltage motor starters and isolation contactors for the 8 main ventilation supply fans. The isolation contactors will shutdown the supply fans in event of earthquake

  6. Propulsion Wheel Motor for an Electric Vehicle

    Science.gov (United States)

    Figuered, Joshua M. (Inventor); Herrera, Eduardo (Inventor); Waligora, Thomas M. (Inventor); Bluethmann, William J. (Inventor); Farrell, Logan Christopher (Inventor); Lee, Chunhao J. (Inventor); Vitale, Robert L. (Inventor); Winn, Ross Briant (Inventor); Eggleston, IV, Raymond Edward (Inventor); Guo, Raymond (Inventor); hide

    2016-01-01

    A wheel assembly for an electric vehicle includes a wheel rim that is concentrically disposed about a central axis. A propulsion-braking module is disposed within an interior region of the wheel rim. The propulsion-braking module rotatably supports the wheel rim for rotation about the central axis. The propulsion-braking module includes a liquid cooled electric motor having a rotor rotatable about the central axis, and a stator disposed radially inside the rotor relative to the central axis. A motor-wheel interface hub is fixedly attached to the wheel rim, and is directly attached to the rotor for rotation with the rotor. The motor-wheel interface hub directly transmits torque from the electric motor to the wheel rim at a 1:1 ratio. The propulsion-braking module includes a drum brake system having an electric motor that rotates a cam device, which actuates the brake shoes.

  7. Motor competence and physical fitness in adolescents.

    Science.gov (United States)

    Gísladóttir, Ordís; Haga, Monika; Sigmundsson, Hermundur

    2014-01-01

    In this study we examined the relationship between physical fitness and motor competence in adolescents aged 15 to 16 years. A sample of 94 adolescents participated in the study. To test motor competence, the Movement Assessment Battery for Children-2 was used. Physical fitness was assessed using the following test items: standing broad jump, 20-m dash, reduced Cooper test, and sit-and-reach test. The results revealed a significant but weak relationship (0.248) between motor competence and physical fitness for the whole sample. More specifically, the correlation between the 2 variables was significant for girls (0.353) but not for boys (0.248). The relatively weak relationship between motor competence and physical fitness suggests that motor competence might not be critical in adolescents to maintain their physical fitness.

  8. Electronically commutated motors for vehicle applications

    Science.gov (United States)

    Echolds, E. F.

    1980-02-01

    Two permanent magnet electronically commutated motors for electric vehicle traction are discussed. One, based on existing technology, produces 23 kW (peak) at 26,000 rpm, and 11 kW continuous at 18,000 rpm. The motor has a conventional design: a four-pole permanent magnet rotor and a three-phase stator similar to those used on ordinary induction motors. The other, advanced technology motor, is rated at 27 kW (peak) at 14,000 rpm, and 11 kW continuous at 10,500 rpm. The machine employs a permanent magnet rotor and a novel ironless stator design in an axial air gap, homopolar configuration. Comparison of the new motors with conventional brush type machines indicates potential for substantial cost savings.

  9. Motor Skill Learning and Corticospinal Excitability

    DEFF Research Database (Denmark)

    Christiansen, Lasse

    Background Motor skill learning (MSL) is the persistent increase in performance of a skill obtained through practice. This process is associated with changes throughout the central nervous system. One of these is a change in corticospinal excitability (CSE) assessable with Transcranial Magnetic...... a novel visuomotor skill. I hypothesized that changes in CSE accompanying long-term motor practice relate to the process of learning rather than repetitive practice on an acquired skill and investigated this by incrementally increasing task difficulty and thus postponing saturation of learning....... Furthermore, we aimed to investigate the feasibility of applying paired associative stimulation to the investigation of learning-dependent motor cortical plasticity by comparing the transient increase in CSE accompanying motor skill learning to the associative plasticity induced by pairing electrical motor...

  10. Application and Perspectives of Multiphase Induction Motors

    Directory of Open Access Journals (Sweden)

    Benas Kundrotas

    2012-04-01

    Full Text Available The article considers the areas of applying multiphase induction motors. Their advantages against three phase motors have become the main reason for employing them in multiphase drives. The paper deals with the six-phase induction motor having two similar three phase windings in the stator shifted by 30 degrees in space and three phase windings in the rotor. Differential equations for this motor are presented and transformed to dq synchronous reference frame. The transformed equations are expressed in a matrix form and solved by MATLAB software using the Dormand-Prince (ode45 method. The transient characteristics of the torque, speed and current of the six-phase induction motor are calculated and discussed.Article in Lithuanian

  11. Modulation of motor performance and motor learning by transcranial direct current stimulation.

    Science.gov (United States)

    Reis, Janine; Fritsch, Brita

    2011-12-01

    Transcranial direct current stimulation (tDCS) has shown preliminary success in improving motor performance and motor learning in healthy individuals, and restitution of motor deficits in stroke patients. This brief review highlights some recent work. Within the past years, behavioural studies have confirmed and specified the timing and polarity specific effects of tDCS on motor skill learning and motor adaptation. There is strong evidence that timely co-application of (hand/arm) training and anodal tDCS to the contralateral M1 can improve motor learning. Improvements in motor function as measured by clinical scores have been described for combined tDCS and training in stroke patients. For this purpose, electrode montages have been modified with respect to interhemispheric imbalance after brain injury. Cathodal tDCS applied to the unlesioned M1 or bihemispheric M1 stimulation appears to be well tolerated and useful to induce improvements in motor function. Mechanistic studies in humans and animals are discussed with regard to physiological motor learning. tDCS is well tolerated, easy to use and capable of inducing lasting improvements in motor function. This method holds promise for the rehabilitation of motor disabilities, although acute studies in patients with brain injury are so far lacking.

  12. Task-dependent activation of distinct fast and slow(er) motor pathways during motor imagery.

    Science.gov (United States)

    Keller, Martin; Taube, Wolfgang; Lauber, Benedikt

    2018-02-22

    Motor imagery and actual movements share overlapping activation of brain areas but little is known about task-specific activation of distinct motor pathways during mental simulation of movements. For real contractions, it was demonstrated that the slow(er) motor pathways are activated differently in ballistic compared to tonic contractions but it is unknown if this also holds true for imagined contractions. The aim of the present study was to assess the activity of fast and slow(er) motor pathways during mentally simulated movements of ballistic and tonic contractions. H-reflexes were conditioned with transcranial magnetic stimulation at different interstimulus intervals to assess the excitability of fast and slow(er) motor pathways during a) the execution of tonic and ballistic contractions, b) motor imagery of these contraction types, and c) at rest. In contrast to the fast motor pathways, the slow(er) pathways displayed a task-specific activation: for imagined ballistic as well as real ballistic contractions, the activation was reduced compared to rest whereas enhanced activation was found for imagined tonic and real tonic contractions. This study provides evidence that the excitability of fast and slow(er) motor pathways during motor imagery resembles the activation pattern observed during real contractions. The findings indicate that motor imagery results in task- and pathway-specific subliminal activation of distinct subsets of neurons in the primary motor cortex. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

  13. Motor experience with a sport-specific implement affects motor imagery

    Science.gov (United States)

    Zhu, Hua; Shen, Cheng; Zhang, Jian

    2018-01-01

    The present study tested whether sport-specific implements facilitate motor imagery, whereas nonspecific implements disrupt motor imagery. We asked a group of basketball players (experts) and a group of healthy controls (novices) to physically perform (motor execution) and mentally simulate (motor imagery) basketball throws. Subjects produced motor imagery when they were holding a basketball, a volleyball, or nothing. Motor imagery performance was measured by temporal congruence, which is the correspondence between imagery and execution times estimated as (imagery time minus execution time) divided by (imagery time plus execution time), as well as the vividness of motor imagery. Results showed that experts produced greater temporal congruence and vividness of kinesthetic imagery while holding a basketball compared to when they were holding nothing, suggesting a facilitation effect from sport-specific implements. In contrast, experts produced lower temporal congruence and vividness of kinesthetic imagery while holding a volleyball compared to when they were holding nothing, suggesting the interference effect of nonspecific implements. Furthermore, we found a negative correlation between temporal congruence and the vividness of kinesthetic imagery in experts while holding a basketball. On the contrary, the implement manipulation did not modulate the temporal congruence of novices. Our findings suggest that motor representation in experts is built on motor experience associated with specific-implement use and thus was subjected to modulation of the implement held. We conclude that sport-specific implements facilitate motor imagery, whereas nonspecific implements could disrupt motor representation in experts. PMID:29719738

  14. Survival motor neuron protein in motor neurons determines synaptic integrity in spinal muscular atrophy.

    Science.gov (United States)

    Martinez, Tara L; Kong, Lingling; Wang, Xueyong; Osborne, Melissa A; Crowder, Melissa E; Van Meerbeke, James P; Xu, Xixi; Davis, Crystal; Wooley, Joe; Goldhamer, David J; Lutz, Cathleen M; Rich, Mark M; Sumner, Charlotte J

    2012-06-20

    The inherited motor neuron disease spinal muscular atrophy (SMA) is caused by deficient expression of survival motor neuron (SMN) protein and results in severe muscle weakness. In SMA mice, synaptic dysfunction of both neuromuscular junctions (NMJs) and central sensorimotor synapses precedes motor neuron cell death. To address whether this synaptic dysfunction is due to SMN deficiency in motor neurons, muscle, or both, we generated three lines of conditional SMA mice with tissue-specific increases in SMN expression. All three lines of mice showed increased survival, weights, and improved motor behavior. While increased SMN expression in motor neurons prevented synaptic dysfunction at the NMJ and restored motor neuron somal synapses, increased SMN expression in muscle did not affect synaptic function although it did improve myofiber size. Together these data indicate that both peripheral and central synaptic integrity are dependent on motor neurons in SMA, but SMN may have variable roles in the maintenance of these different synapses. At the NMJ, it functions at the presynaptic terminal in a cell-autonomous fashion, but may be necessary for retrograde trophic signaling to presynaptic inputs onto motor neurons. Importantly, SMN also appears to function in muscle growth and/or maintenance independent of motor neurons. Our data suggest that SMN plays distinct roles in muscle, NMJs, and motor neuron somal synapses and that restored function of SMN at all three sites will be necessary for full recovery of muscle power.

  15. 2008 South Dakota motor vehicle traffic crash summary

    Science.gov (United States)

    2009-06-01

    The Motor Vehicle Traffic Crash Summary is divided into two main sections, Historical : Trends and 2008 Motor Vehicle Traffic Crash Profile. The Historical Trend section : provides information on alcohol involvement in motor vehicle crashes, severity...

  16. 2010 South Dakota motor vehicle traffic crash summary

    Science.gov (United States)

    2011-01-01

    The Motor Vehicle Traffic Crash Summary is divided into two main sections, Historical Trends and 2010 Motor Vehicle Traffic Crash Profile. The Historical Trend section provides information on alcohol involvement in motor vehicle crashes, severity of ...

  17. 2009 South Dakota motor vehicle traffic crash summary

    Science.gov (United States)

    2010-06-01

    The Motor Vehicle Traffic Crash Summary is divided into two main sections, Historical : Trends and 2009 Motor Vehicle Traffic Crash Profile. The Historical Trend section : provides information on alcohol involvement in motor vehicle crashes, severity...

  18. Brief Overview of Motor Learning and It's Application to Rehabilitation: Part Ⅰ: Motor Learning Theory

    Institute of Scientific and Technical Information of China (English)

    Christopher A Zaino

    2003-01-01

    @@ 1 DEFINITION OF MOTOR LEARNING Motor learning is the study of how we acquire and modify movements.1 The acquisition of motor skills is the process of learning how to do a particular movement (performance), but the real key to therapeutic intervention is being able to affect permanent changes in motor skills via the process of motor learning. Therefore, motor learning is defined as the ability to retain the ability to perform a motor task at a later time. In rehabilitation, it is important to be cognizant of the concepts of acquisition and retention. We can facilitate acquisition,but do little to assist in the retention of the task (learning). Conversely, we can arrange practice such that acquisition is slowed, but we can actually be assisting learning the task. It is important to have a clear goal in mind and work towards the eventual learning of the task to allow full functional use of that skill.

  19. Association Between Gross-Motor and Executive Function Depends on Age and Motor Task Complexity

    DEFF Research Database (Denmark)

    Spedden, Meaghan E; Malling, Anne Sofie B; Andersen, Ken K

    2017-01-01

    The objective was to examine associations between motor and executive function across the adult lifespan and to investigate the role of motor complexity in these associations. Young, middle-aged and older adults (n = 82; 19-83y) performed two gross-motor tasks with different levels of complexity...... and a Stroop-like computer task. Performance was decreased in older adults. The association between motor and cognitive performance was significant for older adults in the complex motor task (p = 0.03, rs = -0.41), whereas no significant associations were found for young or middle-aged groups, suggesting...... that the link between gross-motor and executive function emerges with age and depends on motor complexity....

  20. Motor unit recruitment by size does not provide functional advantages for motor performance.

    Science.gov (United States)

    Dideriksen, Jakob L; Farina, Dario

    2013-12-15

    It is commonly assumed that the orderly recruitment of motor units by size provides a functional advantage for the performance of movements compared with a random recruitment order. On the other hand, the excitability of a motor neuron depends on its size and this is intrinsically linked to its innervation number. A range of innervation numbers among motor neurons corresponds to a range of sizes and thus to a range of excitabilities ordered by size. Therefore, if the excitation drive is similar among motor neurons, the recruitment by size is inevitably due to the intrinsic properties of motor neurons and may not have arisen to meet functional demands. In this view, we tested the assumption that orderly recruitment is necessarily beneficial by determining if this type of recruitment produces optimal motor output. Using evolutionary algorithms and without any a priori assumptions, the parameters of neuromuscular models were optimized with respect to several criteria for motor performance. Interestingly, the optimized model parameters matched well known neuromuscular properties, but none of the optimization criteria determined a consistent recruitment order by size unless this was imposed by an association between motor neuron size and excitability. Further, when the association between size and excitability was imposed, the resultant model of recruitment did not improve the motor performance with respect to the absence of orderly recruitment. A consistent observation was that optimal solutions for a variety of criteria of motor performance always required a broad range of innervation numbers in the population of motor neurons, skewed towards the small values. These results indicate that orderly recruitment of motor units in itself does not provide substantial functional advantages for motor control. Rather, the reason for its near-universal presence in human movements is that motor functions are optimized by a broad range of innervation numbers.

  1. Efficiency Characteristics of Low Power Hybrid Switched Reluctance Motor

    DEFF Research Database (Denmark)

    Jakobsen, Uffe; Ahn, Jin-Woo

    2009-01-01

    Switched reluctance motors (SRM) are usually considered inferior in terms of efficiency as compared to permanent magnet synchronous motors (PMSM) and brushless DC-motors (BLDC), but less costly. This article presents a test of a 70W hybrid switched reluctance motor (HSRM), that archieves a peak...... efficiency for the motor drive of more than 74%, and an efficiency for the motor of almost 80%....

  2. A Squirrel Cage Type Electric Motor Rotor Assembly.

    Science.gov (United States)

    1996-09-05

    cage motor, but also provides efficiencies approaching those of permanent magnet motors . With the above and other objects in view, as will...and active motor life relative to known permanent magnet motors . Referring to FIG. 4, there is illustrated an alternative embodiment in which...part the.known advantages of a squirrel cage motor, and further provides improved efficiencies approaching those of permanent magnet motors . It is to

  3. An Evaluation of Electric Motors for Ship Propulsion

    Science.gov (United States)

    2003-06-01

    AIM). Permanent magnet motors are more power dense than a comparatively sized in- duction motor. The permanent magnet motor has been chosen to...study. They include the axial flux, the ra- dial flux, and the transverse flux permanent magnet motors . Each motor has its unique advantages...to be ideal for ship propulsion, work is ongoing to develop the PMSM for ship propulsion. Permanent magnet motors are expected to have significant

  4. High-inertia drive motors and their starting characteristics

    International Nuclear Information System (INIS)

    Anon.

    1980-01-01

    The motor for a large reactor coolant pump failed while starting. The motor-application and the motor-failure are discussed in detail. A review of applications of motors for high-inertia drives shows that a motor designed and built to today's industry-standards might be overstressed while experiencing abnormal starting conditions, even though its protection is in accord with accepted practice. The inter-relationship between motor characteristics and characteristics of various types of protection are discussed, briefly. The review concludes that motor specifications and motor standards should be augmented. 1 ref

  5. Motor training reduces surround inhibition in the motor cortex.

    Science.gov (United States)

    Akkad, Haya; Di Stasio, Flavio; Tibold, Robert; Kassavetis, Panagiotis; Rothwell, John C; Edwards, Mark J

    2016-06-01

    Surround inhibition (SI) is thought to facilitate focal contraction of a hand muscle by keeping nearby muscles silent. Unexpectedly, SI is reduced in skilled pianists. We tested whether repeated practice of focal contraction in non-pianists could reduce SI. Motor-evoked potentials were elicited by transcranial magnetic stimulation in the relaxed abductor digiti minimi randomly at the onset and 5s after offset of a 2s focal contraction (10% maximum) of the first dorsal interosseous (FDI). Over 5 blocks of 40 trials participants obtained points for increasing contraction speed and stability in FDI. In a final block, the interval between contractions was varied randomly to increase attention to the task. Over the first 5 blocks, SI declined as performance (points scored) improved. In the final "attention" block SI increased towards baseline without affecting performance. Although SI may be useful during the early stages of learning, skilled focal finger movement does not require SI to prevent activity in non-involved muscles. This could be due to better targeting of the excitatory command to move. Results from the final block suggest that increased attention can re-engage SI when task parameters change. SI is not necessary for successful focal contraction, but may contribute during learning and during attention to task. Copyright © 2016 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.

  6. Fuzzy control of small servo motors

    Science.gov (United States)

    Maor, Ron; Jani, Yashvant

    1993-01-01

    To explore the benefits of fuzzy logic and understand the differences between the classical control methods and fuzzy control methods, the Togai InfraLogic applications engineering staff developed and implemented a motor control system for small servo motors. The motor assembly for testing the fuzzy and conventional controllers consist of servo motor RA13M and an encoder with a range of 4096 counts. An interface card was designed and fabricated to interface the motor assembly and encoder to an IBM PC. The fuzzy logic based motor controller was developed using the TILShell and Fuzzy C Development System on an IBM PC. A Proportional-Derivative (PD) type conventional controller was also developed and implemented in the IBM PC to compare the performance with the fuzzy controller. Test cases were defined to include step inputs of 90 and 180 degrees rotation, sine and square wave profiles in 5 to 20 hertz frequency range, as well as ramp inputs. In this paper we describe our approach to develop a fuzzy as well as PH controller, provide details of hardware set-up and test cases, and discuss the performance results. In comparison, the fuzzy logic based controller handles the non-linearities of the motor assembly very well and provides excellent control over a broad range of parameters. Fuzzy technology, as indicated by our results, possesses inherent adaptive features.

  7. Two-Phase Induction Motor Drives

    Directory of Open Access Journals (Sweden)

    Gholam Reza Arab Markadeh

    2010-10-01

    Full Text Available The lack of variable-speed drives for two (single induction motor is a reality. This article attempts mainly to investigate the reasons for this lack of variable – speed drives. This paper deals with literature survey of various existing converter topologies, which have been proposed for adjustable speed single phase induction motor drives. Various converter topologies have been compared in this paper. Among these converter topologies, the adjustable frequency PWM inverter is the best choice for single-phase induction motor drives. However, adjustable-frequency drives have not been widely used with single-phase Induction motors. The open-loop constant V/F control law cannot be used with the single-phase induction motor drives as it is used with three phase motors. The variation of the operating frequency at lower speed range with constant load torque causes variation in motor's slip. A constant V/F control is suitable only over the upper speed range.

  8. Homopolar dc motor and trapped flux brushless dc motor using high temperature superconductor materials

    Science.gov (United States)

    Crapo, Alan D.; Lloyd, Jerry D.

    1991-03-01

    Two motors have been designed and built for use with high-temperature superconductor (HTSC) materials. They are a homopolar dc motor that uses HTSC field windings and a brushless dc motor that uses bulk HTSC materials to trap flux in steel rotor poles. The HTSC field windings of the homopolar dc motor are designed to operate at 1000 A/sq cm in a 0.010-T field. In order to maximize torque in the homopolar dc motor, an iron magnetic circuit with small air gaps gives maximum flux for minimum Ampere turns in the field. A copper field winding version of the homopolar dc motor has been tested while waiting for 575 A turn HTSC coils. The trapped flux brushless dc motor has been built and is ready to test melt textured bulk HTSC rings that are currently being prepared. The stator of the trapped flux motor will impress a magnetic field in the steel rotor poles with warm HTSC bulk rings. The rings are then cooled to 77 K to trap the flux in the rotor. The motor can then operate as a brushless dc motor.

  9. Permanent magnet DC motor control by using arduino and motor drive module BTS7960

    Science.gov (United States)

    Syukriyadin, S.; Syahrizal, S.; Mansur, G.; Ramadhan, H. P.

    2018-05-01

    This study proposes a control system for permanent magnet DC (PMDC) motor. PMDC drive control system has two critical parameters: control and monitoring. Control system includes rotation speed control and direction of rotation of motor using motor drive module BTS7960. The PWM signal has a fixed frequency of waves with varying duty cycles (between 0% and 100%), so the motor rotation can be regulated gradually using a potentiometer already programmed on the Arduino Uno board. The motor rotation direction setting uses the H-bridge circuit method using a 3-way switch to set the direction of forward-reverse rotation of the motor. The monitoring system includes measurements of rotational speed, current, and voltage. Motor rotation speed can be adjusted from the armature voltage settings through the duty cycle PWM setting so that the motor speed can be increased or decreased by the desired duty cycle. From the unload PMDC motor test results it has also been shown that the torque of the motor is relatively constant when there is a change in speed from low rpm to high rpm or vice versa.

  10. Gene Expression Changes in the Motor Cortex Mediating Motor Skill Learning

    Science.gov (United States)

    Cheung, Vincent C. K.; DeBoer, Caroline; Hanson, Elizabeth; Tunesi, Marta; D'Onofrio, Mara; Arisi, Ivan; Brandi, Rossella; Cattaneo, Antonino; Goosens, Ki A.

    2013-01-01

    The primary motor cortex (M1) supports motor skill learning, yet little is known about the genes that contribute to motor cortical plasticity. Such knowledge could identify candidate molecules whose targeting might enable a new understanding of motor cortical functions, and provide new drug targets for the treatment of diseases which impair motor function, such as ischemic stroke. Here, we assess changes in the motor-cortical transcriptome across different stages of motor skill acquisition. Adult rats were trained on a gradually acquired appetitive reach and grasp task that required different strategies for successful pellet retrieval, or a sham version of the task in which the rats received pellet reward without needing to develop the reach and grasp skill. Tissue was harvested from the forelimb motor-cortical area either before training commenced, prior to the initial rise in task performance, or at peak performance. Differential classes of gene expression were observed at the time point immediately preceding motor task improvement. Functional clustering revealed that gene expression changes were related to the synapse, development, intracellular signaling, and the fibroblast growth factor (FGF) family, with many modulated genes known to regulate synaptic plasticity, synaptogenesis, and cytoskeletal dynamics. The modulated expression of synaptic genes likely reflects ongoing network reorganization from commencement of training till the point of task improvement, suggesting that motor performance improves only after sufficient modifications in the cortical circuitry have accumulated. The regulated FGF-related genes may together contribute to M1 remodeling through their roles in synaptic growth and maturation. PMID:23637843

  11. Motoric subtypes of delirium in geriatric patients

    Directory of Open Access Journals (Sweden)

    Sandeep Grover

    2014-01-01

    Results: On amended DMSS, hyperactive subtype (N = 45; 45.9% was the most common motoric subtype of delirium, followed by hypoactive subtype (N = 23; 23.5%, and mixed subtype (N = 21; 21.4%. On DRS-R-98, all patients fulfilled the criteria of ′acute (temporal onset of symptoms′, ′presence of an underlying physical disorder′ and ′difficulty in attention′. In the total sample, >90% of the patients had disturbances in sleep-wake cycle, orientation and fluctuation of symptoms. The least common symptoms were delusions, visuospatial disturbances and motor retardation. When compared to hypoactive group, significantly higher proportion of patients with hyperactive subtype had delusions, perceptual disturbances, and motor agitation. Whereas, compared to hyperactive subtype, significantly higher proportion of patients with hypoactive subtype had thought process abnormality and motor retardation. When the hyperactive and mixed motoric subtype groups were compared, patients with mixed subtype group had significantly higher prevalence of thought process abnormality and motor retardation. Comparison of hypoactive and mixed subtype revealed significant differences in the frequency of perceptual disturbances, delusions and motor agitation and all these symptoms being found more commonly in patients with the mixed subtype. Severity of symptoms were found to be significantly different across the various motoric subtypes for some of the non-cognitive symptoms, but significant differences were not seen for the cognitive symptoms as assessed on DRS-R-98. Conclusion: In elderly patients, motor subtypes of delirium differ from each other on non-cognitive symptom profile in terms of frequency and severity.

  12. Functional connectivity between somatosensory and motor brain areas predicts individual differences in motor learning by observing.

    Science.gov (United States)

    McGregor, Heather R; Gribble, Paul L

    2017-08-01

    Action observation can facilitate the acquisition of novel motor skills; however, there is considerable individual variability in the extent to which observation promotes motor learning. Here we tested the hypothesis that individual differences in brain function or structure can predict subsequent observation-related gains in motor learning. Subjects underwent an anatomical MRI scan and resting-state fMRI scans to assess preobservation gray matter volume and preobservation resting-state functional connectivity (FC), respectively. On the following day, subjects observed a video of a tutor adapting her reaches to a novel force field. After observation, subjects performed reaches in a force field as a behavioral assessment of gains in motor learning resulting from observation. We found that individual differences in resting-state FC, but not gray matter volume, predicted postobservation gains in motor learning. Preobservation resting-state FC between left primary somatosensory cortex and bilateral dorsal premotor cortex, primary motor cortex, and primary somatosensory cortex and left superior parietal lobule was positively correlated with behavioral measures of postobservation motor learning. Sensory-motor resting-state FC can thus predict the extent to which observation will promote subsequent motor learning. NEW & NOTEWORTHY We show that individual differences in preobservation brain function can predict subsequent observation-related gains in motor learning. Preobservation resting-state functional connectivity within a sensory-motor network may be used as a biomarker for the extent to which observation promotes motor learning. This kind of information may be useful if observation is to be used as a way to boost neuroplasticity and sensory-motor recovery for patients undergoing rehabilitation for diseases that impair movement such as stroke. Copyright © 2017 the American Physiological Society.

  13. Variable-Reluctance Motor For Electric Vehicles

    Science.gov (United States)

    Lang, Jeffrey H.

    1987-01-01

    Report describes research on variable-reluctance electric-motor drive for eventual use in electric-vehicle propulsion. Primary design and performance criteria were torque and power output per unit mass of motor, cost, and drive efficiency. For each criterion, optimized drive design developed, and designs unified to yield single electric-vehicle drive. Scaled-down motor performed as expected. Prototype of paraplegic lift operated by toggle switch and joystick. Lift plugs into household electrical outlet for recharging when not in use.

  14. Investigation of vibration characteristics of electric motors

    Science.gov (United States)

    Bakshis, A. K.; Tamoshyunas, Y. K.

    1973-01-01

    The vibration characteristics of electric motors were analyzed using mathematical statistics methods. The equipment used and the method of conducting the test are described. Curves are developed to show the visualization of the electric motor vibrations in the vertical direction. Additional curves are included to show the amplitude-phase frequency characteristic of dynamic rotor-housing vibrations at the first lug and the same data for the second lug of the electric motor. Mathematical models were created to show the transmission function of the dynamic rotor housing system.

  15. Fleet wide motor asset management program

    International Nuclear Information System (INIS)

    Rosemeier, R.G.; Moxie, J.J.; Mendez, W.E.

    2011-01-01

    The Institute of Nuclear Power Operations (INPO) performed several studies concerning the effects of motor failures on US power production. Problems with aging and the lack of preventative maintenance were noted as major contributors to this production loss. Westinghouse has developed, and successfully utilized, a computer program to assist plants in the decision making process for maximizing the operational availability of their motor fleet. The program considers motors of a fleet as a single, critical to production asset, and aids the operator in decisions regarding the following: 1.) Purchase of spares. 2.) Rewind and refurbishment prioritization. 3.) Long-term budget forecasting. (author)

  16. A dynamical systems approach to motor development.

    Science.gov (United States)

    Kamm, K; Thelen, E; Jensen, J L

    1990-12-01

    The study of motor development has long influenced the clinical practice of physical therapy. We first review the contributions and deficiencies of two traditional maturational and reflex-based models of motor development. Second, we describe basic principles of kinematic and kinetic analyses of movement and show how we have applied these techniques to understand infant stepping and kicking. Third, we propose a theory of motor development based on a dynamical systems perspective that is consistent with our infant studies. Finally, we explore the implications of the model for physical therapists.

  17. Economical motor protection using microcomputer technology

    Energy Technology Data Exchange (ETDEWEB)

    Woodruff, N.

    1983-09-01

    A trend to design new motors closer to their design limits and the high cost of plant shutdown has increased the need for better protection of smaller three phase motors. A single chip microcomputer relay can be applied to replace thermal overloads which are of limited effectiveness on low and medium voltage machines with comprehensive, economical motor protection. The requirement for different protection features and how they are achieved is presented. All the protection features discussed are commercially available in a compact unit that uses a single chip microcomputer.

  18. Two-phase induction motor drives

    DEFF Research Database (Denmark)

    Blaabjerg, Frede; Lungeanu, Florin; Skaug, Kenneth

    2004-01-01

    There is a continuous debate today concerning the ?motor of the future? for low-power applications requiring less than 1-2 kW. The specialists are focusing on superior motors [1] like brushless-dc, permanent-magnet synchronous, or electronically commutated types, because they show improvements...... in efficiency, reliability, torque-per-volume/mass ratio, maintenance, and service life. Despite this effort, the industry seems to be responding with manifest rigidity in changing standard induction motor solutions when considering the installed volume into the field, the investment in manufacturing base...

  19. Three phase full wave dc motor decoder

    Science.gov (United States)

    Studer, P. A. (Inventor)

    1977-01-01

    A three phase decoder for dc motors is disclosed which employs an extremely simple six transistor circuit to derive six properly phased output signals for fullwave operation of dc motors. Six decoding transistors are coupled at their base-emitter junctions across a resistor network arranged in a delta configuration. Each point of the delta configuration is coupled to one of three position sensors which sense the rotational position of the motor. A second embodiment of the invention is disclosed in which photo-optical isolators are used in place of the decoding transistors.

  20. Linear Parameter Varying Control of Induction Motors

    DEFF Research Database (Denmark)

    Trangbæk, Klaus

    The subject of this thesis is the development of linear parameter varying (LPV) controllers and observers for control of induction motors. The induction motor is one of the most common machines in industrial applications. Being a highly nonlinear system, it poses challenging control problems...... for high performance applications. This thesis demonstrates how LPV control theory provides a systematic way to achieve good performance for these problems. The main contributions of this thesis are the application of the LPV control theory to induction motor control as well as various contributions...

  1. Development of a miniature fan motor

    Science.gov (United States)

    Wang, Chien-Chang; Yao, Yeong-Der; Liang, Kun-Yi; Huang, Chung-Chun; Chang, Yu-Choung

    2012-04-01

    A novel compact axial flux fan motor was developed. Such a micromotor could be a potential candidate for using as the cooling solution for the next generation mobile devices, for example, smart phones and pico-projectors. The key parameters of the motor, such as back electromotive force, cogging torque, and axial preload are predicted using finite element method. In addition, new approaches are proposed to measure these items, and the corresponding experimental results are in good agreement with the simulated one. Moreover, the undesired vibration harmonic is successfully suppressed, and the fan motor represents a high static pressure and air flow rate.

  2. Motor Torque Calculations For Electric Vehicle

    Directory of Open Access Journals (Sweden)

    Saurabh Chauhan

    2015-08-01

    Full Text Available Abstract It is estimated that 25 of the total cars across the world will run on electricity by 2025. An important component that is an integral part of all electric vehicles is the motor. The amount of torque that the driving motor delivers is what plays a decisive role in determining the speed acceleration and performance of an electric vehicle. The following work aims at simplifying the calculations required to decide the capacity of the motor that should be used to drive a vehicle of particular specifications.

  3. Energy-efficient electric motors study

    Science.gov (United States)

    1981-03-01

    A survey conducted of purchasers of integral horsepower polyphase motors measured current knowledge of and awareness of energy efficient motors, decision making criteria, information sources, purchase and usage patterns, and related factors. The data obtained were used for the electric motor market penetration analysis. Additionally, a telephone survey was made. The study also provides analyses of distribution channels, commercialization constraints, and the impacts of government programs and rising energy prices. Study findings, conclusions, and recommendations are presented. Sample questionnaires and copies of letters to respondents are presented in appendices as well as descriptions of the methods used.

  4. The motor system and its disorders

    DEFF Research Database (Denmark)

    Rowe, James B; Siebner, Hartwig R

    2012-01-01

    to study safely the processes of neuroplasticity, neural networks and neuropharmacology in stroke and movement disorders and offers a sensitive tool to assess novel therapeutics. In the reverse direction, imaging of clinical populations has promoted innovations in cognitive theory, experimental design...... on the understanding of diverse motor functions, including motor learning, decision making, inhibition and the mirror neuron system. In addition, we show how imaging of the motor system has supported a powerful platform for bidirectional translational neuroscience. In one direction, it has provided the opportunity...

  5. Motor Importance of motor assessment in school children: analysis of the reliability of the motor development scale

    Directory of Open Access Journals (Sweden)

    Kassandra Nunes Amaro

    2010-09-01

    Full Text Available The objective of this study was to investigate the motor performance of school chil-dren aged 6 to 10 years without learning difficulties (n=101, and to analyze the reliability of the Motor Development Scale (MDS (Rosa Neto, 2002. Descriptive statistics with calculation of the mean, standard deviation, and range was used for data analysis. The internal consistency of the MDS was assessed using Cronbach’s alpha coefficient, and the correlation between variables was determined by Pearson’s linear correlation, with p<0.05. The results showed (1 that motor development was within normal limits in 96% of the children, and (2 a high correlation betwe-en chronological age and general motor age, indicating good internal consistency. These data demonstrate the logic and structured design of the MDS, confirming its reliability.

  6. A CFD study of Screw Compressor Motor Cooling Analysis

    Science.gov (United States)

    Branch, S.

    2017-08-01

    Screw compressors use electric motors to drive the male screw rotor. They are cooled by the suction refrigerant vapor that flows around the motor. The thermal conditions of the motor can dramatically influence the performance and reliability of the compressor. The more optimized this flow path is, the better the motor performance. For that reason it is important to understand the flow characteristics around the motor and the motor temperatures. Computational fluid dynamics (CFD) can be used to provide a detailed analysis of the refrigerant’s flow behavior and motor temperatures to identify the undesirable hot spots in the motor. CFD analysis can be used further to optimize the flow path and determine the reduction of hot spots and cooling effect. This study compares the CFD solutions of a motor cooling model to a motor installed with thermocouples measured in the lab. The compressor considered for this study is an R134a screw compressor. The CFD simulation of the motor consists of a detailed breakdown of the stator and rotor components. Orthotropic thermal conductivity material properties are used to represent the simplified motor geometry. In addition, the analysis includes the motor casings of the compressor to draw heat away from the motor by conduction. The study will look at different operating conditions and motor speeds. Finally, the CFD study will investigate the predicted motor temperature change by varying the vapor mass flow rates and motor speed. Recommendations for CFD modeling of such intricate heat transfer phenomenon have thus been proposed.

  7. [Functional and motor gastrointestinal disorders].

    Science.gov (United States)

    Mearin, Fermín; Rey, Enrique; Balboa, Agustín

    2015-09-01

    This article discusses the most interesting studies on functional and motor gastrointestinal disorders presented at Digestive Diseases Week (DDW), 2015. Researchers are still seeking biomarkers for irritable bowel syndrome and have presented new data. One study confirmed that the use of low-dose antidepressants has an antinociceptive effect without altering the psychological features of patients with functional dyspepsia. A contribution that could have immediate application is the use of transcutaneous electroacupuncture, which has demonstrated effectiveness in controlling nausea in patients with gastroparesis. New data have come to light on the importance of diet in irritable bowel syndrome, although the effectiveness of a low-FODMAP diet seems to be losing momentum with time. Multiple data were presented on the long-term efficacy of rifaximin therapy in patients with irritable bowel syndrome and diarrhoea. In addition, among other contributions, and more as a curiosity, a study evaluated the effect of histamine in the diet of patients with irritable bowel syndrome. Copyright © 2015 Elsevier España, S.L.U. All rights reserved.

  8. Solid rocket motor cost model

    Science.gov (United States)

    Harney, A. G.; Raphael, L.; Warren, S.; Yakura, J. K.

    1972-01-01

    A systematic and standardized procedure for estimating life cycle costs of solid rocket motor booster configurations. The model consists of clearly defined cost categories and appropriate cost equations in which cost is related to program and hardware parameters. Cost estimating relationships are generally based on analogous experience. In this model the experience drawn on is from estimates prepared by the study contractors. Contractors' estimates are derived by means of engineering estimates for some predetermined level of detail of the SRM hardware and program functions of the system life cycle. This method is frequently referred to as bottom-up. A parametric cost analysis is a useful technique when rapid estimates are required. This is particularly true during the planning stages of a system when hardware designs and program definition are conceptual and constantly changing as the selection process, which includes cost comparisons or trade-offs, is performed. The use of cost estimating relationships also facilitates the performance of cost sensitivity studies in which relative and comparable cost comparisons are significant.

  9. Motor proteins and molecular motors: how to operate machines at the nanoscale

    International Nuclear Information System (INIS)

    Kolomeisky, Anatoly B

    2013-01-01

    Several classes of biological molecules that transform chemical energy into mechanical work are known as motor proteins or molecular motors. These nanometer-sized machines operate in noisy stochastic isothermal environments, strongly supporting fundamental cellular processes such as the transfer of genetic information, transport, organization and functioning. In the past two decades motor proteins have become a subject of intense research efforts, aimed at uncovering the fundamental principles and mechanisms of molecular motor dynamics. In this review, we critically discuss recent progress in experimental and theoretical studies on motor proteins. Our focus is on analyzing fundamental concepts and ideas that have been utilized to explain the non-equilibrium nature and mechanisms of molecular motors. (topical review)

  10. Individual differences in motor timing and its relation to cognitive and fine motor skills.

    Directory of Open Access Journals (Sweden)

    Håvard Lorås

    Full Text Available The present study investigated the relationship between individual differences in timing movements at the level of milliseconds and performance on selected cognitive and fine motor skills. For this purpose, young adult participants (N = 100 performed a repetitive movement task paced by an auditory metronome at different rates. Psychometric measures included the digit-span and symbol search subtasks from the Wechsler battery as well as the Raven SPM. Fine motor skills were assessed with the Purdue Pegboard test. Motor timing performance was significantly related (mean r = .3 to cognitive measures, and explained both unique and shared variance with information-processing speed of Raven's scores. No significant relations were found between motor timing measures and fine motor skills. These results show that individual differences in cognitive and motor timing performance is to some extent dependent upon shared processing not associated with individual differences in manual dexterity.

  11. Benefit on motor and non-motor behavior in a specialized unit for Parkinson's disease.

    Science.gov (United States)

    Müller, Thomas; Öhm, Gabi; Eilert, Kathrin; Möhr, Katharina; Rotter, Stephanie; Haas, Thomas; Küchler, Matthias; Lütge, Sven; Marg, Marion; Rothe, Hartmut

    2017-06-01

    Treatment of patients with Parkinson's disease in specialized units is quite common in Germany. Data on the benefit of this hospitalization of patients with Parkinson's disease on motor and non-motor symptoms in conjunction with standardized tests are rare. Objective was to determine the efficacy of this therapeutic setting. We scored disease severity and performed clinical tests, respectively, instrumental procedures under standardized conditions in consecutively referred in-patients initially and at the end of their hospital stay. There was a decrease of motor and non-motor symptoms. The extent of improvement of non-motor and motor symptoms correlated to each other. Performance of complex movement sequences became better, whereas execution of simple movement series did not ameliorate. The interval for the timed up and go test went down. We demonstrate the effectiveness of an in-patient stay in a specialized unit for Parkinson's disease. Objective standardized testing supplements subjective clinical scoring with established rating scales.

  12. Fundamental motor skill proficiency is necessary for children's motor activity inclusion

    Directory of Open Access Journals (Sweden)

    José Angelo Barela

    2013-09-01

    Full Text Available Motor development is influenced by many factors such as practice and appropriate instruction, provided by teachers, even in preschool and elementary school. The goal of this paper was to discuss the misconception that maturation underlies children's motor skill development and to show that physical education, even in early years of our school system, is critical to promote proficiency and enrolment of children's in later motor activities. Motor skill development, as a curricular focus, has been marginalized in many of our physical education proposal and in doing so, we have not promote motor competence in our children who lack proficiency to engage and to participate in later motor activities such as sport-related or recreational.

  13. Synchronous motor with soft start element formed between the motor rotor and motor output shaft to successfully synchronize loads that have high inertia and/or high torque

    Science.gov (United States)

    Umans, Stephen D; Nisley, Donald L; Melfi, Michael J

    2014-10-28

    A line-start synchronous motor has a housing, a rotor shaft, and an output shaft. A soft-start coupling portion is operatively coupled to the output shaft and the rotor shaft. The soft-start coupling portion is configurable to enable the synchronous motor to obtain synchronous operation and to drive, at least near synchronous speed during normal steady state operation of the motor, a load having characteristics sufficient to prevent obtaining normal synchronous operation of the motor when the motor is operatively connected to the load in the absence of the soft-start coupling. The synchronous motor is sufficiently rated to obtain synchronous operation and to drive, at least near synchronous speed during normal steady state operation of the motor, a load having characteristics sufficient to prevent obtaining normal synchronous operation of the motor when the motor is operatively connected to the load in the absence of the soft-start coupling.

  14. Auditory-motor interactions in pediatric motor speech disorders: neurocomputational modeling of disordered development.

    Science.gov (United States)

    Terband, H; Maassen, B; Guenther, F H; Brumberg, J

    2014-01-01

    Differentiating the symptom complex due to phonological-level disorders, speech delay and pediatric motor speech disorders is a controversial issue in the field of pediatric speech and language pathology. The present study investigated the developmental interaction between neurological deficits in auditory and motor processes using computational modeling with the DIVA model. In a series of computer simulations, we investigated the effect of a motor processing deficit alone (MPD), and the effect of a motor processing deficit in combination with an auditory processing deficit (MPD+APD) on the trajectory and endpoint of speech motor development in the DIVA model. Simulation results showed that a motor programming deficit predominantly leads to deterioration on the phonological level (phonemic mappings) when auditory self-monitoring is intact, and on the systemic level (systemic mapping) if auditory self-monitoring is impaired. These findings suggest a close relation between quality of auditory self-monitoring and the involvement of phonological vs. motor processes in children with pediatric motor speech disorders. It is suggested that MPD+APD might be involved in typically apraxic speech output disorders and MPD in pediatric motor speech disorders that also have a phonological component. Possibilities to verify these hypotheses using empirical data collected from human subjects are discussed. The reader will be able to: (1) identify the difficulties in studying disordered speech motor development; (2) describe the differences in speech motor characteristics between SSD and subtype CAS; (3) describe the different types of learning that occur in the sensory-motor system during babbling and early speech acquisition; (4) identify the neural control subsystems involved in speech production; (5) describe the potential role of auditory self-monitoring in developmental speech disorders. Copyright © 2014 Elsevier Inc. All rights reserved.

  15. Axial Field Electric Motor and Method

    National Research Council Canada - National Science Library

    Cho, Chahee P

    2007-01-01

    .... A hybrid field, brushless, permanent magnet electric motor utilizing a rotor with two sets of permanent magnets oriented such that the flux produced by the two sets of magnets is perpendicular to each...

  16. The Neuronal Network Orchestration behind Motor Behaviors

    DEFF Research Database (Denmark)

    Petersen, Peter Christian

    to motoneurons during rhythmic motor behaviors, and specifically the hypothesis that motoneurons receive concurrent excitatory and inhibitory (E/I) inputs. Berg et al. (2007) presented the concurrent hypothesis, which goes against the classical feed forward reciprocal model for spinal motor networks that has...... gained widespread acceptance. We developed an adult turtle preparation where the spinal motor network was intact, which also allowed us to perform intracellular recordings from motoneurons during rhythmic motor activity. We estimated the synaptic excitatory and inhibitory conductances by two individual...... (Buzsáki and Mizuseki, 2014). Roxin et al. (2011) detailed the firing rate distribution in networks in the balanced regime, and found it to be similar to a lognormal distribution and describing the data from the population studies very well. Our experimental observations and analysis are in agreement...

  17. Energy-efficient electric motors study

    Energy Technology Data Exchange (ETDEWEB)

    1981-03-23

    The study identifies the industrial decision makers, investigated the information they needed to know, how they can best be reached, and the motivating factors for purchasing energy-efficient electric motors. A survey was conducted of purchasers of integral horsepower polyphase motors. The survey measured current knowledge of and awareness of energy-efficient motors, decision-making criteria, information sources, purchase and usage patterns, and related factors. The survey data were used for the electric motor market penetration analysis. Additionally, a telephone survey was made. The study also provides analyses of distribution channels, commercialization constraints, and the impacts of government programs and rising energy prices. A description of study findings, conclusions, and recommendations is presented. Sample questionnaires and copies of letters to respondents are presented in 3 appendices. Appendices D and E contain descriptions of the methods used. (MCW)

  18. Cryogenic Rotary Piezoelectric Motor, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Piezoelectric motors operate on the principal of high frequency oscillation of high force precision ceramic elements. The high power oscillations are converted to...

  19. Motor aphasia after cervical myelography with Metrizamide

    International Nuclear Information System (INIS)

    Boeker, D.K.; Sartor, K.; Winkler, D.; Allgemeines Krankenhaus Altona, Hamburg

    1980-01-01

    Two cases of transient motor aphasia after cervical myelography with Metrizamide are described. A possible mechanism is thought to be prolonged contact of contrast with a brain already damaged by a pre-existing vascular abnormality. (orig.) [de

  20. Electronically commutated dc motors for electric vehicles

    Science.gov (United States)

    Maslowski, E. A.

    1981-01-01

    A motor development program to explore the feasibility of electronically commutated dc motors (also known as brushless) for electric cars is described. Two different design concepts and a number of design variations based on these concepts are discussed. One design concept is based on a permanent magnet, medium speed, machine rated at 7000 to 9000 rpm, and powered via a transistor inverter power conditioner. The other concept is based on a permanent magnet, high speed, machine rated at 22,000 to 26,000 rpm, and powered via a thyristor inverter power conditioner. Test results are presented for a medium speed motor and a high speed motor each of which have been fabricated using samarium cobalt permanent magnet material.