WorldWideScience

Sample records for profiles results show

  1. Identification of genes showing differential expression profile ...

    Indian Academy of Sciences (India)

    Suppression subtractive hybridization was used to identify genes showing differential expression profile associated withgrowth rate in skeletal muscle tissue of Landrace weanling pig. Two subtracted cDNA populations were generated from mus-culus longissimus muscle tissues of selected pigs with extreme expected ...

  2. Identification of genes showing differential expression profile ...

    Indian Academy of Sciences (India)

    firmed difference in expression profiles of the identified genes in musculus longissimus muscle tissues between the two Lan- ..... Discussion. The EEF1A2, TSG101 and TTN identified as upregulated genes in high-growth group have been reported to be involved in myotube survival and .... cDNA probes and libraries. Proc.

  3. Electronic cigarette solutions and resultant aerosol profiles.

    Science.gov (United States)

    Herrington, Jason S; Myers, Colton

    2015-10-30

    Electronic cigarettes (e-cigarettes) are growing in popularity exponentially. Despite their ever-growing acceptance, their aerosol has not been fully characterized. The current study focused on evaluating e-cigarette solutions and their resultant aerosol for potential differences. A simple sampling device was developed to draw e-cigarette aerosol into a multi-sorbent thermal desorption (TD) tube, which was then thermally extracted and analyzed via a gas chromatography (GC) mass spectrometry (GC-MS) method. This novel application provided detectable levels of over one hundred fifteen volatile organic compounds (VOCs) and semivolatile organic compounds (SVOCs) from a single 40mL puff. The aerosol profiles from four commercially available e-cigarettes were compared to their respective solution profiles with the same GC-MS method. Solution profiles produced upwards of sixty four unidentified and identified (some only tentatively) constituents and aerosol profiles produced upwards of eighty two compounds. Results demonstrated distinct analyte profiles between liquid and aerosol samples. Most notably, formaldehyde, acetaldehyde, acrolein, and siloxanes were found in the aerosol profiles; however, these compounds were never present in the solutions. These results implicate the aerosolization process in the formation of compounds not found in solutions; have potential implications for human health; and stress the need for an emphasis on electronic cigarette aerosol testing. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  4. Fingerprinting using extrolite profiles and physiological data shows sub-specific groupings of Penicillium crustosum strains

    DEFF Research Database (Denmark)

    Sonjak, Silva; Frisvad, Jens Christian; Gunde-Cimerman, Nina

    2009-01-01

    if the strains investigated already showed slight adaptations to the selected external parameter. In contrast, PCA analyses of the extrolite data showed groupings of the strains according to their origins and known physiological differences. These groupings are in full agreement with the clustering obtained...... water activity. Principal component analysis (PCA) was performed using micromorphological data, temperature- and water-dependent growth rates, and extrolite profiles obtained by HPLC analysis. The micromorphological data were less informative, while the growth-rate data were informative only...

  5. Phenolic Acids from Wheat Show Different Absorption Profiles in Plasma: A Model Experiment with Catheterized Pigs

    DEFF Research Database (Denmark)

    Nørskov, Natalja; Hedemann, Mette Skou; Theil, Peter Kappel

    2013-01-01

    , their concentrations in the plasma and the absorption profiles differed between cinnamic and benzoic acid derivatives. Cinnamic acids derivatives such as ferulic acid and caffeic acid had maximum plasma concentration of 82 ± 20 and 200 ± 7 nM, respectively, and their absorption profiles differed depending on the diet...... consumed. Benzoic acid derivatives showed low concentration in the plasma (acid, with a plasma concentration (4 ± 0.4 μM), much higher than the other plant phenolic acids, likely because it is an intermediate in the phenolic acid metabolism......The concentration and absorption of the nine phenolic acids of wheat were measured in a model experiment with catheterized pigs fed whole grain wheat and wheat aleurone diets. Six pigs in a repeated crossover design were fitted with catheters in the portal vein and mesenteric artery to study...

  6. Embryonic carcinoma cells show specific dielectric resistance profiles during induced differentiation.

    Directory of Open Access Journals (Sweden)

    Simin Öz

    Full Text Available Induction of differentiation in cancer stem cells by drug treatment represents an important approach for cancer therapy. The understanding of the mechanisms that regulate such a forced exit from malignant pluripotency is fundamental to enhance our knowledge of tumour stability. Certain nucleoside analogues, such as 2'-deoxy-5-azacytidine and 1β-arabinofuranosylcytosine, can induce the differentiation of the embryonic cancer stem cell line NTERA 2 D1 (NT2. Such induced differentiation is associated with drug-dependent DNA-damage, cellular stress and the proteolytic depletion of stem cell factors. In order to further elucidate the mode of action of these nucleoside drugs, we monitored differentiation-specific changes of the dielectric properties of growing NT2 cultures using electric cell-substrate impedance sensing (ECIS. We measured resistance values of untreated and retinoic acid treated NT2 cells in real-time and compared their impedance profiles to those of cell populations triggered to differentiate with several established substances, including nucleoside drugs. Here we show that treatment with retinoic acid and differentiation-inducing drugs can trigger specific, concentration-dependent changes in dielectric resistance of NT2 cultures, which can be observed as early as 24 hours after treatment. Further, low concentrations of nucleoside drugs induce differentiation-dependent impedance values comparable to those obtained after retinoic acid treatment, whereas higher concentrations induce proliferation defects. Finally, we show that impedance profiles of substance-induced NT2 cells and those triggered to differentiate by depletion of the stem cell factor OCT4 are very similar, suggesting that reduction of OCT4 levels has a dominant function for differentiation induced by nucleoside drugs and retinoic acid. The data presented show that NT2 cells have specific dielectric properties, which allow the early identification of differentiating

  7. Data showing phenotypic profile of uropathogenic Escherichia coli isolates from sepsis patients

    Directory of Open Access Journals (Sweden)

    Vivek Verma

    2016-06-01

    Full Text Available Bacterial virulence factors (VFs influence the site and severity of urinary tract infections (UTI and further leading to sepsis infection. Phenotypic characterisation of VFs specific to sepsis Escherichia coli strains has not been characterized in Indian population till date. In this data article, we have described important VFs of uropathogenic E. coli (UPEC that is P fim, Type-1 fim, cell surface hydrophobicity, mannose resistant haemagglutination/mannose sensitive haemagglutination (MRHA/MSHA expression and α-haemolysin production. The data includes a profile of the five VFs investigated in E. coli isolates from sepsis patients (N=78 and control group (N=50 from non-sepsis subjects. We found that P fim phenotype was expressed in 25.3% of E. coli isolates from sepsis patients, whereas Type-1 fimbriae was detected in 30.5%. Cell surface hydrophobicity phenotype was present in 30.5%, α-haemolysin in 26.3% and MRHA/MSHA in 22.1% of sepsis E. coli isolates. None of the control E. coli isolates showed presence of these phenotypes. The combined phenotypic profile of all the five VFs was significantly higher in sepsis patients as compared to the control group.

  8. Urinary Metabolite Profiles in Premature Infants Show Early Postnatal Metabolic Adaptation and Maturation

    Directory of Open Access Journals (Sweden)

    Sissel J. Moltu

    2014-05-01

    Full Text Available Objectives: Early nutrition influences metabolic programming and long-term health. We explored the urinary metabolite profiles of 48 premature infants (birth weight < 1500 g randomized to an enhanced or a standard diet during neonatal hospitalization. Methods: Metabolomics using nuclear magnetic resonance spectroscopy (NMR was conducted on urine samples obtained during the first week of life and thereafter fortnightly. Results: The intervention group received significantly higher amounts of energy, protein, lipids, vitamin A, arachidonic acid and docosahexaenoic acid as compared to the control group. Enhanced nutrition did not appear to affect the urine profiles to an extent exceeding individual variation. However, in all infants the glucogenic amino acids glycine, threonine, hydroxyproline and tyrosine increased substantially during the early postnatal period, along with metabolites of the tricarboxylic acid cycle (succinate, oxoglutarate, fumarate and citrate. The metabolite changes correlated with postmenstrual age. Moreover, we observed elevated threonine and glycine levels in first-week urine samples of the small for gestational age (SGA; birth weight < 10th percentile for gestational age as compared to the appropriate for gestational age infants. Conclusion: This first nutri-metabolomics study in premature infants demonstrates that the physiological adaptation during the fetal-postnatal transition as well as maturation influences metabolism during the breastfeeding period. Elevated glycine and threonine levels were found in the first week urine samples of the SGA infants and emerged as potential biomarkers of an altered metabolic phenotype.

  9. Metabolic profiles show specific mitochondrial toxicities in vitro in myotube cells

    Energy Technology Data Exchange (ETDEWEB)

    Xu Qiuwei, E-mail: qiuwei_xu@merck.com; Vu, Heather; Liu Liping; Wang, Ting-Chuan; Schaefer, William H. [Merck Research Laboratories (United States)

    2011-04-15

    Mitochondrial toxicity has been a serious concern, not only in preclinical drug development but also in clinical trials. In mitochondria, there are several distinct metabolic processes including fatty acid {beta}-oxidation, the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation (OXPHOS), and each process contains discrete but often intimately linked steps. Interruption in any one of those steps can cause mitochondrial dysfunction. Detection of inhibition to OXPHOS can be complicated in vivo because intermediate endogenous metabolites can be recycled in situ or circulated systemically for metabolism in other organs or tissues. Commonly used assays for evaluating mitochondrial function are often applied to ex vivo or in vitro samples; they include various enzymatic or protein assays, as well as functional assays such as measurement of oxygen consumption rate, membrane potential, or acidification rates. Metabolomics provides quantitative profiles of overall metabolic changes that can aid in the unraveling of explicit biochemical details of mitochondrial inhibition while providing a holistic view and heuristic understanding of cellular bioenergetics. In this paper, we showed the application of quantitative NMR metabolomics to in vitro myotube cells treated with mitochondrial toxicants, rotenone and antimycin A. The close coupling of the TCA cycle to the electron transfer chain (ETC) in OXPHOS enables specific diagnoses of inhibition to ETC complexes by discrete biochemical changes in the TCA cycle.

  10. Where is the science? What will it take to show that nutrient profiling systems work?

    Science.gov (United States)

    Townsend, Marilyn S

    2010-04-01

    Nutrient profiling is defined as the science of ranking or categorizing foods on the basis of their nutritional composition. Validity is a general term meaning accuracy. Nutrient profiling systems in the United States have not undergone any systematic validation effort to assess their accuracy against a comparison measure or group of measures. Different types of validation studies should be conducted: content, face, convergent, criterion, and predictive. This article provides a conceptual framework for establishing the validity of nutrient profiling systems with the desired objective of assisting US consumers with food selection to improve diet quality. For a profiling system to work successfully in the American marketplace, it must function well with consumers from most or all cultural groups, from all racial groups, and with low-literate as well as highly literate people. Emphasis should be placed on conducting different types of validation studies and multiple studies with different subpopulation groups. The use of consistent standards to assess the accuracy and usefulness of multiple profiling systems is imperative to successfully identify a nutrient profiling intervention that will have the potential to lead to improved diet quality and eventually to an improved health status in US consumers.

  11. Cytokine profiles show heterogeneity of interferon-β response in multiple sclerosis patients

    DEFF Research Database (Denmark)

    Hegen, Harald; Adrianto, Indra; Lessard, Christopher J

    2016-01-01

    OBJECTIVE: To evaluate serum cytokine profiles for their utility to determine the heterogeneous responses to interferon (IFN)-β treatment in patients with multiple sclerosis (MS). METHODS: Patients with relapsing-remitting MS (RRMS) or clinically isolated syndrome receiving de novo IFN-β treatment...... were included in this prospective, observational study. Number of relapses and changes in disability were assessed 2 years prior to and 2 years after initiation of treatment. Sera were collected at baseline and after 3 months on therapy. Cytokine levels in sera were assessed by Luminex multiplex assays...... and no worsening of disability. Each subset also had differential changes in cytokine levels after 3 months of IFN-β treatment. CONCLUSIONS: There is heterogeneity in the immunologic pathways of the RRMS population, which correlates with IFN-β response....

  12. Comparative Transcriptional Profiling of Contrasting Rice Genotypes Shows Expression Differences during Arsenic Stress

    Directory of Open Access Journals (Sweden)

    Arti Rai

    2015-07-01

    Full Text Available Accumulation of arsenic (As in rice ( L. grain is a serious concern worldwide. Long-term exposure to As affects nutritional status in rice grain and is associated with higher rates of skin, bladder, and lung cancers, and heart disease. Genotypic variations in rice for As accumulation or tolerance are prevalent and are regulated by genetic and environmental factors. To understand molecular networks involved in As accumulation, genome-wide expression analysis was performed in roots of low- and high-As accumulating rice genotypes (LARGs and HARGs. Six rice genotypes with contrasting As accumulation potential and tolerance were used in this study. Genome-wide expression analysis suggested their differential response against As stress. This study suggests up- and downregulation of a number of unique genes involved in various pathways and biological processes in response to As stress in rice genotypes. A comparison of gene expression profiles, principal component analysis, and -means clustering suggests that an independent pathway is operating during As stress tolerance or accumulation in contrasting genotypes. It was also observed that the differential behavior of genotype, Nayanmoni, from other LARGs might be due to its different genetic background. -motif profiling of As-induced coexpressed genes in diverse rice genotypes led to the identification of unique -motifs present in differentially expressed genes. This study suggests that the genetic mechanism regulating the differential As accumulation in different genotypes may not be dependent on gene expression at the transcriptional level. However, many genes identified in this study can be analyzed and used for marker–trait associations related to As accumulation in diverse genotypes around the world.

  13. Different methods to quantify Listeria monocytogenesbiofilms cells showed different profile in their viability

    Directory of Open Access Journals (Sweden)

    Lizziane Kretli Winkelströter

    2015-03-01

    Full Text Available Listeria monocytogenes is a foodborne pathogen able to adhere and to form biofilms in several materials commonly present in food processing plants. The aim of this study was to evaluate the resistance of Listeria monocytogenes attached to abiotic surface, after treatment with sanitizers, by culture method, microscopy and Quantitative Real Time Polymerase Chain Reaction (qPCR. Biofilms of L. monocytogenes were obtained in stainless steel coupons immersed in Brain Heart Infusion Broth, under agitation at 37 °C for 24 h. The methods selected for this study were based on plate count, microscopic count with the aid of viability dyes (CTC-DAPI, and qPCR. Results of culture method showed that peroxyacetic acid was efficient to kill sessile L. monocytogenes populations, while sodium hypochlorite was only partially effective to kill attached L. monocytogenes (p < 0.05. When, viability dyes (CTC/DAPI combined with fluorescence microscopy and qPCR were used and lower counts were found after treatments (p < 0.05. Selective quantification of viable cells of L. monocytogenes by qPCR using EMA revelead that the pre-treatment with EMA was not appropriate since it also inhibited amplification of DNA from live cells by ca. 2 log. Thus, the use of CTC counts was the best method to count viable cells in biofilms.

  14. Allergic asthmatics show divergent lipid mediator profiles from healthy controls both at baseline and following birch pollen provocation.

    Directory of Open Access Journals (Sweden)

    Susanna L Lundström

    Full Text Available Asthma is a respiratory tract disorder characterized by airway hyper-reactivity and chronic inflammation. Allergic asthma is associated with the production of allergen-specific IgE and expansion of allergen-specific T-cell populations. Progression of allergic inflammation is driven by T-helper type 2 (Th2 mediators and is associated with alterations in the levels of lipid mediators.Responses of the respiratory system to birch allergen provocation in allergic asthmatics were investigated. Eicosanoids and other oxylipins were quantified in the bronchoalveolar lumen to provide a measure of shifts in lipid mediators associated with allergen challenge in allergic asthmatics.Eighty-seven lipid mediators representing the cyclooxygenase (COX, lipoxygenase (LOX and cytochrome P450 (CYP metabolic pathways were screened via LC-MS/MS following off-line extraction of bronchoalveolar lavage fluid (BALF. Multivariate statistics using OPLS were employed to interrogate acquired oxylipin data in combination with immunological markers.Thirty-two oxylipins were quantified, with baseline asthmatics possessing a different oxylipin profile relative to healthy individuals that became more distinct following allergen provocation. The most prominent differences included 15-LOX-derived ω-3 and ω-6 oxylipins. Shared-and-Unique-Structures (SUS-plot modeling showed a correlation (R(2 = 0.7 between OPLS models for baseline asthmatics (R(2Y[cum] = 0.87, Q(2[cum] = 0.51 and allergen-provoked asthmatics (R(2Y[cum] = 0.95, Q(2[cum] = 0.73, with the majority of quantified lipid mediators and cytokines contributing equally to both groups. Unique structures for allergen provocation included leukotrienes (LTB(4 and 6-trans-LTB(4, CYP-derivatives of linoleic acid (epoxides/diols, and IL-10.Differences in asthmatic relative to healthy profiles suggest a role for 15-LOX products of both ω-6 and ω-3 origin in allergic inflammation. Prominent differences at baseline levels indicate

  15. Efferent Vestibular Neurons Show Homogenous Discharge Output But Heterogeneous Synaptic Input Profile In Vitro.

    Directory of Open Access Journals (Sweden)

    Miranda A Mathews

    Full Text Available Despite the importance of our sense of balance we still know remarkably little about the central control of the peripheral balance system. While previous work has shown that activation of the efferent vestibular system results in modulation of afferent vestibular neuron discharge, the intrinsic and synaptic properties of efferent neurons themselves are largely unknown. Here we substantiate the location of the efferent vestibular nucleus (EVN in the mouse, before characterizing the input and output properties of EVN neurons in vitro. We made transverse serial sections through the brainstem of 4-week-old mice, and performed immunohistochemistry for calcitonin gene-related peptide (CGRP and choline acetyltransferase (ChAT, both expressed in the EVN of other species. We also injected fluorogold into the posterior canal and retrogradely labelled neurons in the EVN of ChAT:: tdTomato mice expressing tdTomato in all cholinergic neurons. As expected the EVN lies dorsolateral to the genu of the facial nerve (CNVII. We then made whole-cell current-, and voltage-clamp recordings from visually identified EVN neurons. In current-clamp, EVN neurons display a homogeneous discharge pattern. This is characterized by a high frequency burst of action potentials at the onset of a depolarizing stimulus and the offset of a hyperpolarizing stimulus that is mediated by T-type calcium channels. In voltage-clamp, EVN neurons receive either exclusively excitatory or inhibitory inputs, or a combination of both. Despite this heterogeneous mixture of inputs, we show that synaptic inputs onto EVN neurons are predominantly excitatory. Together these findings suggest that the inputs onto EVN neurons, and more specifically the origin of these inputs may underlie EVN neuron function.

  16. Plasma and serum lipidomics of healthy white adults shows characteristic profiles by subjects' gender and age.

    Directory of Open Access Journals (Sweden)

    Masaki Ishikawa

    Full Text Available Blood is a commonly used biofluid for biomarker discovery. Although blood lipid metabolites are considered to be potential biomarker candidates, their fundamental properties are not well characterized. We aimed to (1 investigate the matrix type (serum vs. plasma that may be preferable for lipid biomarker exploration, (2 elucidate age- and gender-associated differences in lipid metabolite levels, and (3 examine the stability of lipid metabolites in matrix samples subjected to repeated freeze-thaw cycles. Using liquid chromatography-mass spectrometry, we performed lipidomic analyses for fasting plasma and serum samples for four groups (15 subjects/group of young and elderly (25-34 and 55-64 years old, respectively males and females and for an additional aliquot of samples from young males, which were subjected to repeated freeze-thaw cycles. Lysophosphatidylcholine and diacylglycerol levels were higher in serum than in plasma samples, suggesting that the clotting process influences serum lipid metabolite levels. Gender-associated differences highlighted that the levels of many sphingomyelin species were significantly higher in females than in males, irrespective of age and matrix (plasma and serum. Age-associated differences were more prominent in females than in males, and in both matrices, levels of many triacylglycerols were significantly higher in elderly females than in young females. Plasma and serum levels of most lipid metabolites were reduced by freeze-thawing. Our results indicate that plasma is an optimal matrix for exploring lipid biomarkers because it represents the original properties of an individual's blood sample. In addition, the levels of some blood lipid species of healthy adults showed gender- and age-associated differences; thus, this should be considered during biomarker exploration and its application in diagnostics. Our fundamental findings on sample selection and handling procedures for measuring blood lipid metabolites

  17. Planck intermediate results: XVI. Profile likelihoods for cosmological parameters

    DEFF Research Database (Denmark)

    Bartlett, J.G.; Cardoso, J.-F.; Delabrouille, J.

    2014-01-01

    We explore the 2013 Planck likelihood function with a high-precision multi-dimensional minimizer (Minuit). This allows a refinement of the CDM best-fit solution with respect to previously-released results, and the construction of frequentist confidence intervals using profile likelihoods. The agr...

  18. Metabolic profiling of plasma amino acids shows that histidine increases following the consumption of pork

    Directory of Open Access Journals (Sweden)

    Samman S

    2014-06-01

    Full Text Available Samir Samman,1 Ben Crossett,2 Miles Somers,1 Kirstine J Bell,1 Nicole T Lai,1,3 David R Sullivan,3 Peter Petocz4 1Discipline of Nutrition and Metabolism, 2Discipline of Proteomics and Biotechnology, School of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia; 3Department of Clinical Biochemistry, Royal Prince Alfred Hospital, Sydney, NSW, Australia; 4Department of Statistics, Macquarie University, Sydney, NSW, Australia Abstract: Amino acid (AA status is determined by factors including nutrition, metabolic rate, and interactions between the metabolism of AA, carbohydrates, and lipids. Analysis of the plasma AA profile, together with markers of glucose and lipid metabolism, will shed light on metabolic regulation. The objectives of this study were to investigate the acute responses to the consumption of meals containing either pork (PM or chicken (CM, and to identify relationships between plasma AA and markers of glycemic and lipemic control. A secondary aim was to explore AA predictors of plasma zinc concentrations. Ten healthy adults participated in a postprandial study on two separate occasions. In a randomized cross-over design, participants consumed PM or CM. The concentrations of 21 AA, glucose, insulin, triglycerides, nonesterified fatty acids, and zinc were determined over 5 hours postprandially. The meal composition did not influence glucose, insulin, triglyceride, nonesterified fatty acid, or zinc concentrations. Plasma histidine was higher following the consumption of PM (P=0.014, with consistently higher changes observed after 60 minutes (P<0.001. Greater percentage increases were noted at limited time points for valine and leucine + isoleucine in those who consumed CM compared to PM. In linear regression, some AAs emerged as predictors of the metabolic responses, irrespective of the meal that was consumed. The present study demonstrates that a single meal of PM or CM produces a differential profile of AA in the

  19. Gun shows and gun violence: fatally flawed study yields misleading results.

    Science.gov (United States)

    Wintemute, Garen J; Hemenway, David; Webster, Daniel; Pierce, Glenn; Braga, Anthony A

    2010-10-01

    A widely publicized but unpublished study of the relationship between gun shows and gun violence is being cited in debates about the regulation of gun shows and gun commerce. We believe the study is fatally flawed. A working paper entitled "The Effect of Gun Shows on Gun-Related Deaths: Evidence from California and Texas" outlined this study, which found no association between gun shows and gun-related deaths. We believe the study reflects a limited understanding of gun shows and gun markets and is not statistically powered to detect even an implausibly large effect of gun shows on gun violence. In addition, the research contains serious ascertainment and classification errors, produces results that are sensitive to minor specification changes in key variables and in some cases have no face validity, and is contradicted by 1 of its own authors' prior research. The study should not be used as evidence in formulating gun policy.

  20. Detailed characterization of multiple myeloma circulating tumor cells shows unique phenotypic, cytogenetic, functional, and circadian distribution profile.

    Science.gov (United States)

    Paiva, Bruno; Paino, Teresa; Sayagues, Jose-Maria; Garayoa, Mercedes; San-Segundo, Laura; Martín, Montserrat; Mota, Ines; Sanchez, María-Luz; Bárcena, Paloma; Aires-Mejia, Irene; Corchete, Luis; Jimenez, Cristina; Garcia-Sanz, Ramon; Gutierrez, Norma C; Ocio, Enrique M; Mateos, Maria-Victoria; Vidriales, Maria-Belen; Orfao, Alberto; San Miguel, Jesús F

    2013-11-21

    Circulating myeloma tumor cells (CTCs) as defined by the presence of peripheral blood (PB) clonal plasma cells (PCs) are a powerful prognostic marker in multiple myeloma (MM). However, the biological features of CTCs and their pathophysiological role in MM remains unexplored. Here, we investigate the phenotypic, cytogenetic, and functional characteristics as well as the circadian distribution of CTCs vs paired bone marrow (BM) clonal PCs from MM patients. Our results show that CTCs typically represent a unique subpopulation of all BM clonal PCs, characterized by downregulation (P < .05) of integrins (CD11a/CD11c/CD29/CD49d/CD49e), adhesion (CD33/CD56/CD117/CD138), and activation molecules (CD28/CD38/CD81). Fluorescence in situ hybridization analysis of fluorescence-activated cell sorter-sorted CTCs also unraveled different cytogenetic profiles vs paired BM clonal PCs. Moreover, CTCs were mostly quiescent and associated with higher clonogenic potential when cocultured with BM stromal cells. Most interestingly, CTCs showed a circadian distribution which fluctuates in a similar pattern to that of CD34(+) cells, and opposite to stromal cell-derived factor 1 plasma levels and corresponding surface expression of CXC chemokine receptor 4 on clonal PCs, suggesting that in MM, CTCs may egress to PB to colonize/metastasize other sites in the BM during the patients' resting period.

  1. Gene expression profiling in the Cynomolgus macaque Macaca fascicularis shows variation within the normal birth range

    Directory of Open Access Journals (Sweden)

    Vickers Mark H

    2011-10-01

    Full Text Available Abstract Background Although an adverse early-life environment has been linked to an increased risk of developing the metabolic syndrome, the molecular mechanisms underlying altered disease susceptibility as well as their relevance to humans are largely unknown. Importantly, emerging evidence suggests that these effects operate within the normal range of birth weights and involve mechanisms of developmental palsticity rather than pathology. Method To explore this further, we utilised a non-human primate model Macaca fascicularis (Cynomolgus macaque which shares with humans the same progressive history of the metabolic syndrome. Using microarray we compared tissues from neonates in the average birth weight (50-75th centile to those of lower birth weight (5-25th centile and studied the effect of different growth trajectories within the normal range on gene expression levels in the umbilical cord, neonatal liver and skeletal muscle. Results We identified 1973 genes which were differentially expressed in the three tissue types between average and low birth weight animals (P Conclusion These differences in gene expression levels between animals in the upper and lower percentiles of the normal birth weight range may point towards early life metabolic adaptations that in later life result in differences in disease risk.

  2. Helicobacter pylori strains from a Nigerian cohort show divergent antibiotic resistance rates and a uniform pathogenicity profile.

    Directory of Open Access Journals (Sweden)

    Ute Harrison

    Full Text Available Antibiotic resistance in Helicobacter pylori is a factor preventing its successful eradication. Particularly in developing countries, resistance against commonly used antibiotics is widespread. Here, we present an epidemiological study from Nigeria with 111 isolates. We analyzed the associated disease outcome, and performed a detailed characterization of these isolated strains with respect to their antibiotic susceptibility and their virulence characteristics. Furthermore, statistical analysis was performed on microbiological data as well as patient information and the results of the gastroenterological examination. We found that the variability concerning the production of virulence factors between strains was minimal, with 96.4% of isolates being CagA-positive and 92.8% producing detectable VacA levels. In addition, high frequency of bacterial resistance was observed for metronidazole (99.1%, followed by amoxicillin (33.3%, clarithromycin (14.4% and tetracycline (4.5%. In conclusion, this study indicated that the infection rate of H. pylori infection within the cohort in the present study was surprisingly low (36.6%. Furthermore, an average gastric pathology was observed by histological grading and bacterial isolates showed a uniform pathogenicity profile while indicating divergent antibiotic resistance rates.

  3. Application of bone scans for prostate cancer staging: Which guideline shows better result?

    Science.gov (United States)

    Chong, Ari; Hwang, Insang; Ha, Jung-min; Yu, Seong Hyeon; Hwang, Eu Chang; Yu, Ho Song; Kim, Sun Ouck; Jung, Seung-Il; Kang, Taek Won; Kwon, Dong Deuk; Park, Kwangsung

    2014-01-01

    Introduction: We evaluated the accuracy of current guidelines by analyzing bone scan results and clinical parameters of patients with prostate cancer to determine the optimal guideline for predicting bone metastasis. Methods: We retrospectively analyzed patients who were diagnosed with prostate cancer and who underwent a bone scan. Bone metastasis was confirmed by bone scan results with clinical and radiological follow-up. Serum prostate-specific antigen, Gleason score, percent of positive biopsy core, clinical staging and bone scan results were analyzed. We analyzed diagnostic performance in predicting bone metastasis of the guidelines of the European Association of Urology (EAU), American Urological Association (AUA), and the National Comprehensive Cancer Network (NCCN) guidelines as well as Briganti’s classification and regression tree (CART). We also compared the percent of positive biopsy core between patients with and without bone metastases. Results: A total 167 of 806 patients had bone metastases. Receiver operating curve analysis revealed that the AUA and EAU guidelines were better for detecting bone metastases than were Briganti’s CART and NCCN. No significant difference was observed between AUA and EAU guidelines. Patients with bone metastases had a higher percent positive core than did patients without metastasis (the cut-off value >55.6). Conclusion: The EAU and AUA guidelines showed better results than did Briganti’s CART and NCCN for predicting bone metastasis in the enrolled patients. A bone scan is strongly recommended for patients who have a higher percent positive core and who meet the EAU and AUA guidelines. PMID:25210554

  4. [Mini-open Approach Shows Good Results in Treatment of Femoroacetabular Impingement].

    Science.gov (United States)

    Kirschbaum, Stephanie Maria; Sitterlee, Frank; Perka, Carsten; Müller, Michael

    2017-04-01

    Background Femoroacetabular impingement (FAI) describes a painful, abnormal (bony) contact between the femoral neck and acetabulum, which, if left untreated, contributes to early osteoarthritis (OA) of the hip. Despite its flat learning curve and long operation times, hip arthroscopy has been used increasingly often in recent years in the surgery of femoroacetabular impingement. The mini-open technique (MOT) offers a possible alternative that addresses the abnormal morphological prominence and allows minimally invasive clearance of hip motion. There is still a lack of information on the influence of the greater degree of soft tissue intrusion inherent to the mini-open approach. Therefore we decided to evaluate the medium-term outcome of the mini-open technique and to compare the results to those of the arthroscopic technique reported in the literature. Patients, Material und Methods 99 patients (105 hips, 40 women, 59 men) underwent mini-open surgical treatment for FAI between 2005 and 2012 and were followed-up for an average of 40.5 months. The evaluation focused on the pre- and postoperative pain intensity (NRS: numeric rating scale), the clinical and functional outcomes such as ROM (range of motion), HOS (Hip Outcome Score) and mHHS (modified Harris Hip Score), changes in athletic behaviour and the satisfaction of the patients. Radiological analysis (α-angle, anterior head-neck-offset (HNO), head-neck-offset ratio, ossification, grade of OA) was performed using preoperative, immediate postoperative as well as follow-up radiographs. Finally our results were compared to those reported for the arthroscopic technique in the literature. Results The average pain level decreased from 6.5 ± 2.3 to 2.3 ± 2.6 (p impact and more patients were performing low-impact sports after surgery. Flexion (113°±16 to 117°±16) and internal rotation (12°±8 to 25°±12) improved significantly. α-angle reduction from 62°±11 to 42°± 9 (p < 0.01) was observed

  5. Long-Term Trial Results Show No Mortality Benefit from Annual Prostate Cancer Screening

    Science.gov (United States)

    Thirteen year follow-up data from the Prostate, Lung, Colorectal and Ovarian (PLCO) cancer screening trial show higher incidence but similar mortality among men screened annually with the prostate-specific antigen (PSA) test and digital rectal examination

  6. Results from a survey of the South African GISc community show ...

    African Journals Online (AJOL)

    Serena Coetzee

    spatial data in digital or graphic form for legal, social, political, educational, and design purposes. May work with Geographic Information Systems (GIS). ... Figure 1 shows that gender and population group transformation are well underway but do not. (yet) correlate with the demographics of South Africa's population ...

  7. Data for behavioral results and brain regions showing a time effect during pair-association retrieval

    Directory of Open Access Journals (Sweden)

    Koji Jimura

    2016-09-01

    Full Text Available The current data article provides behavioral and neuroimaging data for the research article "Relatedness-dependent rapid development of brain activity in anterior temporal cortex during pair-association retrieval” (Jimura et al., 2016 [1]. Behavioral performance is provided in a table. Fig. 2 of the article is based on this table. Brain regions showing time effect are provided in a table. A statistical activation map for the time effect is shown in Fig. 3C of the article.

  8. Determination of the vertical electron-density profile in ionospheric tomography: experimental results

    Directory of Open Access Journals (Sweden)

    C. N. Mitchell

    Full Text Available The reconstruction of the vertical electron-density profile is a fundamental problem in ionospheric tomography. Lack of near-horizontal ray paths limits the information available on the vertical profile, so that the resultant image of electron density is biased in a horizontal sense. The vertical profile is of great importance as it affects the authenticity of the entire tomographic image. A new method is described whereby the vertical profile is selected using relative total-electron-content measurements. The new reconstruction process has been developed from modelling studies. A range of background ionospheres, representing many possible peak heights, scale heights and electron densities are formed from a Chapman profile on the bottomside with a range of topside profiles. The iterative reconstruction process is performed on all of these background ionospheres and a numerical selection criterion employed to select the final image. The resulting tomographic images show excellent agreement in electron density when compared with independent verification provided by the EISCAT radar.

  9. Results from a survey of the South African GISc community show ...

    African Journals Online (AJOL)

    This article presents results of a survey by the Geo-information Society of South Africa (GISSA) to gain a better understanding of who the members of the South ... members of the GISc community fulfil roles of data analysis and interpretation, together with data acquisition, data management, and/or visualization/mapping.

  10. Comb-push ultrasound shear elastography of breast masses: initial results show promise.

    Directory of Open Access Journals (Sweden)

    Max Denis

    Full Text Available To evaluate the performance of Comb-push Ultrasound Shear Elastography (CUSE for classification of breast masses.CUSE is an ultrasound-based quantitative two-dimensional shear wave elasticity imaging technique, which utilizes multiple laterally distributed acoustic radiation force (ARF beams to simultaneously excite the tissue and induce shear waves. Female patients who were categorized as having suspicious breast masses underwent CUSE evaluations prior to biopsy. An elasticity estimate within the breast mass was obtained from the CUSE shear wave speed map. Elasticity estimates of various types of benign and malignant masses were compared with biopsy results.Fifty-four female patients with suspicious breast masses from our ongoing study are presented. Our cohort included 31 malignant and 23 benign breast masses. Our results indicate that the mean shear wave speed was significantly higher in malignant masses (6 ± 1.58 m/s in comparison to benign masses (3.65 ± 1.36 m/s. Therefore, the stiffness of the mass quantified by the Young's modulus is significantly higher in malignant masses. According to the receiver operating characteristic curve (ROC, the optimal cut-off value of 83 kPa yields 87.10% sensitivity, 82.61% specificity, and 0.88 for the area under the curve (AUC.CUSE has the potential for clinical utility as a quantitative diagnostic imaging tool adjunct to B-mode ultrasound for differentiation of malignant and benign breast masses.

  11. Comb-push ultrasound shear elastography of breast masses: initial results show promise.

    Science.gov (United States)

    Denis, Max; Mehrmohammadi, Mohammad; Song, Pengfei; Meixner, Duane D; Fazzio, Robert T; Pruthi, Sandhya; Whaley, Dana H; Chen, Shigao; Fatemi, Mostafa; Alizad, Azra

    2015-01-01

    To evaluate the performance of Comb-push Ultrasound Shear Elastography (CUSE) for classification of breast masses. CUSE is an ultrasound-based quantitative two-dimensional shear wave elasticity imaging technique, which utilizes multiple laterally distributed acoustic radiation force (ARF) beams to simultaneously excite the tissue and induce shear waves. Female patients who were categorized as having suspicious breast masses underwent CUSE evaluations prior to biopsy. An elasticity estimate within the breast mass was obtained from the CUSE shear wave speed map. Elasticity estimates of various types of benign and malignant masses were compared with biopsy results. Fifty-four female patients with suspicious breast masses from our ongoing study are presented. Our cohort included 31 malignant and 23 benign breast masses. Our results indicate that the mean shear wave speed was significantly higher in malignant masses (6 ± 1.58 m/s) in comparison to benign masses (3.65 ± 1.36 m/s). Therefore, the stiffness of the mass quantified by the Young's modulus is significantly higher in malignant masses. According to the receiver operating characteristic curve (ROC), the optimal cut-off value of 83 kPa yields 87.10% sensitivity, 82.61% specificity, and 0.88 for the area under the curve (AUC). CUSE has the potential for clinical utility as a quantitative diagnostic imaging tool adjunct to B-mode ultrasound for differentiation of malignant and benign breast masses.

  12. FES Training in Aging: interim results show statistically significant improvements in mobility and muscle fiber size

    Directory of Open Access Journals (Sweden)

    Helmut Kern

    2012-03-01

    Full Text Available Aging is a multifactorial process that is characterized by decline in muscle mass and performance. Several factors, including reduced exercise, poor nutrition and modified hormonal metabolism, are responsible for changes in the rates of protein synthesis and degradation that drive skeletal muscle mass reduction with a consequent decline of force generation and mobility functional performances. Seniors with normal life style were enrolled: two groups in Vienna (n=32 and two groups in Bratislava: (n=19. All subjects were healthy and declared not to have any specific physical/disease problems. The two Vienna groups of seniors exercised for 10 weeks with two different types of training (leg press at the hospital or home-based functional electrical stimulation, h-b FES. Demografic data (age, height and weight were recorded before and after the training period and before and after the training period the patients were submitted to mobility functional analyses and muscle biopsies. The mobility functional analyses were: 1. gait speed (10m test fastest speed, in m/s; 2. time which the subject needed to rise from a chair for five times (5x Chair-Rise, in s; 3. Timed –Up-Go- Test, in s; 4. Stair-Test, in s; 5. isometric measurement of quadriceps force (Torque/kg, in Nm/kg; and 6. Dynamic Balance in mm. Preliminary analyses of muscle biopsies from quadriceps in some of the Vienna and Bratislava patients present morphometric results consistent with their functional behaviors. The statistically significant improvements in functional testings here reported demonstrates the effectiveness of h-b FES, and strongly support h-b FES, as a safe home-based method to improve contractility and performances of ageing muscles.

  13. Identification of genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig.

    Science.gov (United States)

    Komatsu, Yuuta; Sukegawa, Shin; Yamashita, Mai; Katsuda, Naoki; Tong, Bin; Ohta, Takeshi; Kose, Hiroyuki; Yamada, Takahisa

    2016-06-01

    Suppression subtractive hybridization was used to identify genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig. Two subtracted cDNA populations were generated from musculus longissimus muscle tissues of selected pigs with extreme expected breeding values at the age of 100 kg. Three upregulated genes (EEF1A2, TSG101 and TTN) and six downregulated genes (ATP5B, ATP5C1, COQ3, HADHA, MYH1 and MYH7) in pig with genetic propensity for higher growth rate were identified by sequence analysis of 12 differentially expressed clones selected by differential screening following the generation of the subtracted cDNA population. Real-time PCR analysis confirmed difference in expression profiles of the identified genes in musculus longissimus muscle tissues between the two Landrace weanling pig groups with divergent genetic propensity for growth rate. Further, differential expression of the identified genes except for the TTN was validated by Western blot analysis. Additionally, the eight genes other than the ATP5C1 colocalized with the same chromosomal positions as QTLs that have been previously identified for growth rate traits. Finally, the changes of expression predicted from gene function suggested association of upregulation of expression of the EEF1A2, TSG101 and TTN genes and downregulation of the ATP5B, ATP5C1, COQ3, HADHA, MYH1 and MYH7 gene expression with increased growth rate. The identified genes will provide an important insight in understanding the molecular mechanism underlying growth rate in Landrace pig breed.

  14. Missouri Marketing Education Competency Profile. Cross-Referenced to Marketing Education Resources and the Missouri Show-Me Standards. Revised.

    Science.gov (United States)

    Missouri Univ., Columbia. Instructional Materials Lab.

    This packet consists of explanatory materials on the Missouri Marketing Education Competency Profile and related information. Section 1 describes the mission of marketing education, marketing occupations, curriculum framework, and curriculum alignment for the 1996 profile. Section 2 addresses 1996 revisions to the Missouri Marketing Education Core…

  15. Speed and direction response profiles of neurons in macaque MT and MST show modest constraint line tuning.

    Science.gov (United States)

    Duijnhouwer, Jacob; Noest, André J; Lankheet, Martin J M; van den Berg, Albert V; van Wezel, Richard J A

    2013-01-01

    Several models of heading detection during smooth pursuit rely on the assumption of local constraint line tuning to exist in large scale motion detection templates. A motion detector that exhibits pure constraint line tuning responds maximally to any 2D-velocity in the set of vectors that can be decomposed into the central, or classic, preferred velocity (the shortest vector that still yields the maximum response) and any vector orthogonal to that. To test this assumption, we measured the firing rates of isolated middle temporal (MT) and medial superior temporal (MST) neurons to random dot stimuli moving in a range of directions and speeds. We found that as a function of 2D velocity, the pooled responses were best fit with a 2D Gaussian profile with a factor of elongation, orthogonal to the central preferred velocity, of roughly 1.5 for MST and 1.7 for MT. This means that MT and MST cells are more sharply tuned for speed than they are for direction; and that they indeed show some level of constraint line tuning. However, we argue that the observed elongation is insufficient to achieve behavioral heading discrimination accuracy on the order of 1-2 degrees as reported before.

  16. Transcriptome profiling of low temperature-treated cassava apical shoots showed dynamic responses of tropical plant to cold stress

    Directory of Open Access Journals (Sweden)

    An Dong

    2012-02-01

    Full Text Available Abstract Background Cassava is an important tropical root crop adapted to a wide range of environmental stimuli such as drought and acid soils. Nevertheless, it is an extremely cold-sensitive tropical species. Thus far, there is limited information about gene regulation and signalling pathways related to the cold stress response in cassava. The development of microarray technology has accelerated the study of global transcription profiling under certain conditions. Results A 60-mer oligonucleotide microarray representing 20,840 genes was used to perform transcriptome profiling in apical shoots of cassava subjected to cold at 7°C for 0, 4 and 9 h. A total of 508 transcripts were identified as early cold-responsive genes in which 319 sequences had functional descriptions when aligned with Arabidopsis proteins. Gene ontology annotation analysis identified many cold-relevant categories, including 'Response to abiotic and biotic stimulus', 'Response to stress', 'Transcription factor activity', and 'Chloroplast'. Various stress-associated genes with a wide range of biological functions were found, such as signal transduction components (e.g., MAP kinase 4, transcription factors (TFs, e.g., RAP2.11, and reactive oxygen species (ROS scavenging enzymes (e.g., catalase 2, as well as photosynthesis-related genes (e.g., PsaL. Seventeen major TF families including many well-studied members (e.g., AP2-EREBP were also involved in the early response to cold stress. Meanwhile, KEGG pathway analysis uncovered many important pathways, such as 'Plant hormone signal transduction' and 'Starch and sucrose metabolism'. Furthermore, the expression changes of 32 genes under cold and other abiotic stress conditions were validated by real-time RT-PCR. Importantly, most of the tested stress-responsive genes were primarily expressed in mature leaves, stem cambia, and fibrous roots rather than apical buds and young leaves. As a response to cold stress in cassava, an increase

  17. ASD and schizophrenia show distinct developmental profiles in common genetic overlap with population-based social communication difficulties.

    Science.gov (United States)

    St Pourcain, B; Robinson, E B; Anttila, V; Sullivan, B B; Maller, J; Golding, J; Skuse, D; Ring, S; Evans, D M; Zammit, S; Fisher, S E; Neale, B M; Anney, R J L; Ripke, S; Hollegaard, M V; Werge, T; Ronald, A; Grove, J; Hougaard, D M; Børglum, A D; Mortensen, P B; Daly, M J; Davey Smith, G

    2017-01-03

    Difficulties in social communication are part of the phenotypic overlap between autism spectrum disorders (ASD) and schizophrenia. Both conditions follow, however, distinct developmental patterns. Symptoms of ASD typically occur during early childhood, whereas most symptoms characteristic of schizophrenia do not appear before early adulthood. We investigated whether overlap in common genetic influences between these clinical conditions and impairments in social communication depends on the developmental stage of the assessed trait. Social communication difficulties were measured in typically-developing youth (Avon Longitudinal Study of Parents and Children, N⩽5553, longitudinal assessments at 8, 11, 14 and 17 years) using the Social Communication Disorder Checklist. Data on clinical ASD (PGC-ASD: 5305 cases, 5305 pseudo-controls; iPSYCH-ASD: 7783 cases, 11 359 controls) and schizophrenia (PGC-SCZ2: 34 241 cases, 45 604 controls, 1235 trios) were either obtained through the Psychiatric Genomics Consortium (PGC) or the Danish iPSYCH project. Overlap in genetic influences between ASD and social communication difficulties during development decreased with age, both in the PGC-ASD and the iPSYCH-ASD sample. Genetic overlap between schizophrenia and social communication difficulties, by contrast, persisted across age, as observed within two independent PGC-SCZ2 subsamples, and showed an increase in magnitude for traits assessed during later adolescence. ASD- and schizophrenia-related polygenic effects were unrelated to each other and changes in trait-disorder links reflect the heterogeneity of genetic factors influencing social communication difficulties during childhood versus later adolescence. Thus, both clinical ASD and schizophrenia share some genetic influences with impairments in social communication, but reveal distinct developmental profiles in their genetic links, consistent with the onset of clinical symptoms.Molecular Psychiatry advance online

  18. Preliminary Model Results of Beach Profile Dynamics with Stratigraphy

    Science.gov (United States)

    Reniers, A. J.; Koktas, M.; Gallagher, E. L.; Wadman, H. M.; Brodie, K. L.; Johnson, B. D.; McNinch, J.

    2014-12-01

    The presence of spatial variation in grain size within the surf and swash zone is often ignored in numerical modeling whereas Upon closer inspection, a broad range of grain sizes is visible on a beach. This could potentially lead to a significant mismatch between predictions and observations of profile evolution given the strong sensitivity of sediment transport formulae to the grain size. To explore this in more detail, numerical simulations with XBeach have been performed to simulate the observations of changes in beach profile and stratigraphy within the swash zone at Duck, NC, under a range of wave and tidal conditions (see presentations by Wadman et al., and Gallagher et al. for complementary information on the observations at this conference). The research focus is to establish the morphodynamic response to the sediment dynamics at short and longer time scales in the presence of stratigraphy. A better understanding of the mechanisms and subsequently improved modeling will provide more accurate predictions of the morphodynamic response of the beach during moderate and extreme conditions. It will also help in the interpretation of sediment layering of the beach to relate to past extreme storms on geological time scales.

  19. Mouse gastric tumor models with prostaglandin E2 pathway activation show similar gene expression profiles to intestinal-type human gastric cancer

    Directory of Open Access Journals (Sweden)

    Oshima Masanobu

    2009-12-01

    Full Text Available Abstract Background Gastric cancers are generally classified into better differentiated intestinal-type tumor and poorly differentiated diffuse-type one according to Lauren's histological categorization. Although induction of prostaglandin E2 pathway promotes gastric tumors in mice in cooperation with deregulated Wnt or BMP signalings, it has remained unresolved whether the gastric tumor mouse models recapitulate either of human gastric cancer type. This study assessed the similarity in expression profiling between gastric tumors of transgenic mice and various tissues of human cancers to find best-fit human tumors for the transgenic mice models. Results Global expression profiling initially found gastric tumors from COX-2/mPGES-1 (C2mE-related transgenic mice (K19-C2mE, K19-Wnt1/C2mE, and K19-Nog/C2mE resembled gastric cancers among the several tissues of human cancers including colon, breast, lung and gastric tumors. Next, classification of the C2mE-related transgenic mice by a gene signature to distinguish human intestinal- and diffuse-type tumors showed C2mE-related transgenic mice were more similar to intestinal-type compared with diffuse one. We finally revealed that induction of Wnt pathway cooperating with the prostaglandin E2 pathway in mice (K19-Wnt1/C2mE mice further reproduce features of human gastric intestinal-type tumors. Conclusion We demonstrated that C2mE-related transgenic mice show significant similarity to intestinal-type gastric cancer when analyzed by global expression profiling. These results suggest that the C2mE-related transgenic mice, especially K19-Wnt1/C2mE mice, serve as a best-fit model to study molecular mechanism underlying the tumorigenesis of human gastric intestinal-type cancers.

  20. Progesterone profiles around the time of insemination do not show clear differences between of pregnant and not pregnant dairy cows

    DEFF Research Database (Denmark)

    Gorzecka, Justyna; Codrea, Marius Cosmin; Friggens, Nicolas C

    2011-01-01

    differences between the groups. However, the analysis correctly classified 75% of true pregnant cows. Conversely, only 60% of not pregnant animals were classified as such by the discriminate analysis. Individual analysis of progesterone profile features in pregnant and not pregnant groups of estrous cycles...

  1. A Mouse Model of Hyperproliferative Human Epithelium Validated by Keratin Profiling Shows an Aberrant Cytoskeletal Response to Injury

    Directory of Open Access Journals (Sweden)

    Samal Zhussupbekova

    2016-07-01

    Full Text Available A validated animal model would assist with research on the immunological consequences of the chronic expression of stress keratins KRT6, KRT16, and KRT17, as observed in human pre-malignant hyperproliferative epithelium. Here we examine keratin gene expression profile in skin from mice expressing the E7 oncoprotein of HPV16 (K14E7 demonstrating persistently hyperproliferative epithelium, in nontransgenic mouse skin, and in hyperproliferative actinic keratosis lesions from human skin. We demonstrate that K14E7 mouse skin overexpresses stress keratins in a similar manner to human actinic keratoses, that overexpression is a consequence of epithelial hyperproliferation induced by E7, and that overexpression further increases in response to injury. As stress keratins modify local immunity and epithelial cell function and differentiation, the K14E7 mouse model should permit study of how continued overexpression of stress keratins impacts on epithelial tumor development and on local innate and adaptive immunity.

  2. Individuals with agenesis of the corpus callosum show sensory processing differences as measured by the sensory profile.

    Science.gov (United States)

    Demopoulos, Carly; Arroyo, Monica S; Dunn, Winnie; Strominger, Zoe; Sherr, Elliott H; Marco, Elysa

    2015-09-01

    Given reports of high pain thresholds and reduced auditory response in individuals with agenesis of the corpus callosum (AgCC), this study investigated whether affected participants report atypical experiences and behaviors on a well-established sensory processing measure. Fourteen participants with AgCC (ages 11-59) completed the Adolescent/Adult Sensory Profile (Brown & Dunn, 2001). Sensory profile scales were classified as "Atypical" if they were more than 1 standard deviation from the mean. Fifty-seven percent of participants with AgCC reported reduced sensory registration as compared to an expected 16% of the normative sample. Similarly, 50% of the AgCC participants reported atypically increased auditory processing difficulties. Using a well-established sensory processing questionnaire, participants with AgCC reported measurable differences in multiple aspects of sensory processing. The most notable difference was in the quadrant of low sensory registration, suggesting that individuals with AgCC may require sensory information to be presented more slowly or at a higher intensity for adequate processing. The sensory modality that was most affected was the auditory system, which is consistent with increased rates of language disorders and autism spectrum disorders in this population. Understanding sensory processing in individuals with AgCC can both elucidate the role of interhemispheric transfer in the development of intact sensory processing as well as contribute to our knowledge of the role of the corpus callosum in a range of disorders in which sensory processes are impacted. (PsycINFO Database Record (c) 2015 APA, all rights reserved).

  3. Genome-wide expression profiling shows transcriptional reprogramming in Fusarium graminearum by Fusarium graminearum virus 1-DK21 infection

    Directory of Open Access Journals (Sweden)

    Cho Won

    2012-05-01

    Full Text Available Abstract Background Fusarium graminearum virus 1 strain-DK21 (FgV1-DK21 is a mycovirus that confers hypovirulence to F. graminearum, which is the primary phytopathogenic fungus that causes Fusarium head blight (FHB disease in many cereals. Understanding the interaction between mycoviruses and plant pathogenic fungi is necessary for preventing damage caused by F. graminearum. Therefore, we investigated important cellular regulatory processes in a host containing FgV1-DK21 as compared to an uninfected parent using a transcriptional approach. Results Using a 3′-tiling microarray covering all known F. graminearum genes, we carried out genome-wide expression analyses of F. graminearum at two different time points. At the early point of growth of an infected strain as compared to an uninfected strain, genes associated with protein synthesis, including ribosome assembly, nucleolus, and ribosomal RNA processing, were significantly up-regulated. In addition, genes required for transcription and signal transduction, including fungal-specific transcription factors and cAMP signaling, respectively, were actively up-regulated. In contrast, genes involved in various metabolic pathways, particularly in producing carboxylic acids, aromatic amino acids, nitrogen compounds, and polyamines, showed dramatic down-regulation at the early time point. Moreover, genes associated with transport systems localizing to transmembranes were down-regulated at both time points. Conclusion This is the first report of global change in the prominent cellular pathways in the Fusarium host containing FgV1-DK21. The significant increase in transcripts for transcription and translation machinery in fungal host cells seems to be related to virus replication. In addition, significant down-regulation of genes required for metabolism and transporting systems in a fungal host containing the virus appears to be related to the host defense mechanism and fungal virulence. Taken together

  4. Transcriptome profiling of low temperature-treated cassava apical shoots showed dynamic responses of tropical plant to cold stress.

    Science.gov (United States)

    An, Dong; Yang, Jun; Zhang, Peng

    2012-02-10

    Cassava is an important tropical root crop adapted to a wide range of environmental stimuli such as drought and acid soils. Nevertheless, it is an extremely cold-sensitive tropical species. Thus far, there is limited information about gene regulation and signalling pathways related to the cold stress response in cassava. The development of microarray technology has accelerated the study of global transcription profiling under certain conditions. A 60-mer oligonucleotide microarray representing 20,840 genes was used to perform transcriptome profiling in apical shoots of cassava subjected to cold at 7°C for 0, 4 and 9 h. A total of 508 transcripts were identified as early cold-responsive genes in which 319 sequences had functional descriptions when aligned with Arabidopsis proteins. Gene ontology annotation analysis identified many cold-relevant categories, including 'Response to abiotic and biotic stimulus', 'Response to stress', 'Transcription factor activity', and 'Chloroplast'. Various stress-associated genes with a wide range of biological functions were found, such as signal transduction components (e.g., MAP kinase 4), transcription factors (TFs, e.g., RAP2.11), and reactive oxygen species (ROS) scavenging enzymes (e.g., catalase 2), as well as photosynthesis-related genes (e.g., PsaL). Seventeen major TF families including many well-studied members (e.g., AP2-EREBP) were also involved in the early response to cold stress. Meanwhile, KEGG pathway analysis uncovered many important pathways, such as 'Plant hormone signal transduction' and 'Starch and sucrose metabolism'. Furthermore, the expression changes of 32 genes under cold and other abiotic stress conditions were validated by real-time RT-PCR. Importantly, most of the tested stress-responsive genes were primarily expressed in mature leaves, stem cambia, and fibrous roots rather than apical buds and young leaves. As a response to cold stress in cassava, an increase in transcripts and enzyme activities of

  5. A Mouse Model of Hyperproliferative Human Epithelium Validated by Keratin Profiling Shows an Aberrant Cytoskeletal Response to Injury.

    Science.gov (United States)

    Zhussupbekova, Samal; Sinha, Rohit; Kuo, Paula; Lambert, Paul F; Frazer, Ian H; Tuong, Zewen K

    2016-07-01

    A validated animal model would assist with research on the immunological consequences of the chronic expression of stress keratins KRT6, KRT16, and KRT17, as observed in human pre-malignant hyperproliferative epithelium. Here we examine keratin gene expression profile in skin from mice expressing the E7 oncoprotein of HPV16 (K14E7) demonstrating persistently hyperproliferative epithelium, in nontransgenic mouse skin, and in hyperproliferative actinic keratosis lesions from human skin. We demonstrate that K14E7 mouse skin overexpresses stress keratins in a similar manner to human actinic keratoses, that overexpression is a consequence of epithelial hyperproliferation induced by E7, and that overexpression further increases in response to injury. As stress keratins modify local immunity and epithelial cell function and differentiation, the K14E7 mouse model should permit study of how continued overexpression of stress keratins impacts on epithelial tumor development and on local innate and adaptive immunity. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  6. The transcriptional profile of mesenchymal stem cell populations in primary osteoporosis is distinct and shows overexpression of osteogenic inhibitors.

    Directory of Open Access Journals (Sweden)

    Peggy Benisch

    Full Text Available Primary osteoporosis is an age-related disease characterized by an imbalance in bone homeostasis. While the resorptive aspect of the disease has been studied intensely, less is known about the anabolic part of the syndrome or presumptive deficiencies in bone regeneration. Multipotent mesenchymal stem cells (MSC are the primary source of osteogenic regeneration. In the present study we aimed to unravel whether MSC biology is directly involved in the pathophysiology of the disease and therefore performed microarray analyses of hMSC of elderly patients (79-94 years old suffering from osteoporosis (hMSC-OP. In comparison to age-matched controls we detected profound changes in the transcriptome in hMSC-OP, e.g. enhanced mRNA expression of known osteoporosis-associated genes (LRP5, RUNX2, COL1A1 and of genes involved in osteoclastogenesis (CSF1, PTH1R, but most notably of genes coding for inhibitors of WNT and BMP signaling, such as Sclerostin and MAB21L2. These candidate genes indicate intrinsic deficiencies in self-renewal and differentiation potential in osteoporotic stem cells. We also compared both hMSC-OP and non-osteoporotic hMSC-old of elderly donors to hMSC of ∼30 years younger donors and found that the transcriptional changes acquired between the sixth and the ninth decade of life differed widely between osteoporotic and non-osteoporotic stem cells. In addition, we compared the osteoporotic transcriptome to long term-cultivated, senescent hMSC and detected some signs for pre-senescence in hMSC-OP.Our results suggest that in primary osteoporosis the transcriptomes of hMSC populations show distinct signatures and little overlap with non-osteoporotic aging, although we detected some hints for senescence-associated changes. While there are remarkable inter-individual variations as expected for polygenetic diseases, we could identify many susceptibility genes for osteoporosis known from genetic studies. We also found new candidates, e.g. MAB21L

  7. Transcriptome profiling shows gene regulation patterns in a flavonoid pathway in response to exogenous phenylalanine in Boesenbergia rotunda cell culture.

    Science.gov (United States)

    Md-Mustafa, Noor Diyana; Khalid, Norzulaani; Gao, Huan; Peng, Zhiyu; Alimin, Mohd Firdaus; Bujang, Noraini; Ming, Wong Sher; Mohd-Yusuf, Yusmin; Harikrishna, Jennifer A; Othman, Rofina Yasmin

    2014-11-18

    Panduratin A extracted from Boesenbergia rotunda is a flavonoid reported to possess a range of medicinal indications which include anti-dengue, anti-HIV, anti-cancer, antioxidant and anti-inflammatory properties. Boesenbergia rotunda is a plant from the Zingiberaceae family commonly used as a food ingredient and traditional medicine in Southeast Asia and China. Reports on the health benefits of secondary metabolites extracted from Boesenbergia rotunda over the last few years has resulted in rising demands for panduratin A. However large scale extraction has been hindered by the naturally low abundance of the compound and limited knowledge of its biosynthetic pathway. Transcriptome sequencing and digital gene expression (DGE) analysis of native and phenylalanine treated Boesenbergia rotunda cell suspension cultures were carried out to elucidate the key genes differentially expressed in the panduratin A biosynthetic pathway. Based on experiments that show increase in panduratin A production after 14 days post treatment with exogenous phenylalanine, an aromatic amino acid derived from the shikimic acid pathway, total RNA of untreated and 14 days post-phenylalanine treated cell suspension cultures were extracted and sequenced using next generation sequencing technology employing an Illumina-Solexa platform. The transcriptome data generated 101, 043 unigenes with 50, 932 (50.41%) successfully annotated in the public protein databases; including 49.93% (50, 447) in the non-redundant (NR) database, 34.63% (34, 989) in Swiss-Prot, 24,07% (24, 316) in Kyoto Encyclopedia of Genes and Genomes (KEGG) and 16.26% (16, 426) in Clusters of Orthologous Groups (COG). Through DGE analysis, we found that 14, 644 unigenes were up-regulated and 14, 379 unigenes down-regulated in response to exogenous phenylalanine treatment. In the phenylpropanoid pathway leading to the proposed panduratin A production, 2 up-regulated phenylalanine ammonia-lyase (PAL), 3 up-regulated 4-coumaroyl

  8. Effective Filtering of Query Results on Updated User Behavioral Profiles in Web Mining.

    Science.gov (United States)

    Sadesh, S; Suganthe, R C

    2015-01-01

    Web with tremendous volume of information retrieves result for user related queries. With the rapid growth of web page recommendation, results retrieved based on data mining techniques did not offer higher performance filtering rate because relationships between user profile and queries were not analyzed in an extensive manner. At the same time, existing user profile based prediction in web data mining is not exhaustive in producing personalized result rate. To improve the query result rate on dynamics of user behavior over time, Hamilton Filtered Regime Switching User Query Probability (HFRS-UQP) framework is proposed. HFRS-UQP framework is split into two processes, where filtering and switching are carried out. The data mining based filtering in our research work uses the Hamilton Filtering framework to filter user result based on personalized information on automatic updated profiles through search engine. Maximized result is fetched, that is, filtered out with respect to user behavior profiles. The switching performs accurate filtering updated profiles using regime switching. The updating in profile change (i.e., switches) regime in HFRS-UQP framework identifies the second- and higher-order association of query result on the updated profiles. Experiment is conducted on factors such as personalized information search retrieval rate, filtering efficiency, and precision ratio.

  9. Effective Filtering of Query Results on Updated User Behavioral Profiles in Web Mining

    Directory of Open Access Journals (Sweden)

    S. Sadesh

    2015-01-01

    Full Text Available Web with tremendous volume of information retrieves result for user related queries. With the rapid growth of web page recommendation, results retrieved based on data mining techniques did not offer higher performance filtering rate because relationships between user profile and queries were not analyzed in an extensive manner. At the same time, existing user profile based prediction in web data mining is not exhaustive in producing personalized result rate. To improve the query result rate on dynamics of user behavior over time, Hamilton Filtered Regime Switching User Query Probability (HFRS-UQP framework is proposed. HFRS-UQP framework is split into two processes, where filtering and switching are carried out. The data mining based filtering in our research work uses the Hamilton Filtering framework to filter user result based on personalized information on automatic updated profiles through search engine. Maximized result is fetched, that is, filtered out with respect to user behavior profiles. The switching performs accurate filtering updated profiles using regime switching. The updating in profile change (i.e., switches regime in HFRS-UQP framework identifies the second- and higher-order association of query result on the updated profiles. Experiment is conducted on factors such as personalized information search retrieval rate, filtering efficiency, and precision ratio.

  10. Transcriptome profiling in conifers and the PiceaGenExpress database show patterns of diversification within gene families and interspecific conservation in vascular gene expression

    Directory of Open Access Journals (Sweden)

    Raherison Elie

    2012-08-01

    Full Text Available Abstract Background Conifers have very large genomes (13 to 30 Gigabases that are mostly uncharacterized although extensive cDNA resources have recently become available. This report presents a global overview of transcriptome variation in a conifer tree and documents conservation and diversity of gene expression patterns among major vegetative tissues. Results An oligonucleotide microarray was developed from Picea glauca and P. sitchensis cDNA datasets. It represents 23,853 unique genes and was shown to be suitable for transcriptome profiling in several species. A comparison of secondary xylem and phelloderm tissues showed that preferential expression in these vascular tissues was highly conserved among Picea spp. RNA-Sequencing strongly confirmed tissue preferential expression and provided a robust validation of the microarray design. A small database of transcription profiles called PiceaGenExpress was developed from over 150 hybridizations spanning eight major tissue types. In total, transcripts were detected for 92% of the genes on the microarray, in at least one tissue. Non-annotated genes were predominantly expressed at low levels in fewer tissues than genes of known or predicted function. Diversity of expression within gene families may be rapidly assessed from PiceaGenExpress. In conifer trees, dehydrins and late embryogenesis abundant (LEA osmotic regulation proteins occur in large gene families compared to angiosperms. Strong contrasts and low diversity was observed in the dehydrin family, while diverse patterns suggested a greater degree of diversification among LEAs. Conclusion Together, the oligonucleotide microarray and the PiceaGenExpress database represent the first resource of this kind for gymnosperm plants. The spruce transcriptome analysis reported here is expected to accelerate genetic studies in the large and important group comprised of conifer trees.

  11. Colitic scid mice fed Lactobacillus spp. show an ameliorated gut histopathology and an altered cytokine profile by local T cells.

    Science.gov (United States)

    Møller, Peter Lange; Paerregaard, Anders; Gad, Monika; Kristensen, Nanna Ny; Claesson, Mogens Helweg

    2005-09-01

    Scid mice transplanted with CD4 T blast cells develop colitis. We investigated if the disease was influenced in colitic mice treated with antibiotic and fed Lactobacillus spp. Colitic scid mice were treated for 1 week with antibiotics (vancomycin/meropenem) followed or not followed by a 3-week administration of Lactobacillus reuteri DSM-12246 and Lactobacillus rhamnosus 19070-2 at 2x10 live bacteria/mouse/24 hours. After 12 weeks, the rectums were removed for histology, and CD4 T cells from the mesenteric lymph nodes (MLN) were polyclonally activated for cytokine measurements. Irrespective of no treatment or treatments with antibiotics and probiotics, all mice transplanted with T cell blasts lost 10% of their body weight during the 12-week experimental period, whereas the nontransplanted mice had a 10% weight increase (Pprobiotics showed severe gut inflammation, whereas only 2 of the 7 mice fed probiotics showed signs of severe colitis (Pprobiotics. Our data suggest that probiotics added to the drinking water may ameliorate local histopathological changes and influence local cytokine levels in colitic mice but not alter the colitis-associated weight loss.

  12. DNA Methylation Profiling of Human Prefrontal Cortex Neurons in Heroin Users Shows Significant Difference between Genomic Contexts of Hyper- and Hypomethylation and a Younger Epigenetic Age

    Directory of Open Access Journals (Sweden)

    Alexey Kozlenkov

    2017-05-01

    Full Text Available We employed Illumina 450 K Infinium microarrays to profile DNA methylation (DNAm in neuronal nuclei separated by fluorescence-activated sorting from the postmortem orbitofrontal cortex (OFC of heroin users who died from heroin overdose (N = 37, suicide completers (N = 22 with no evidence of heroin use and from control subjects who did not abuse illicit drugs and died of non-suicide causes (N = 28. We identified 1298 differentially methylated CpG sites (DMSs between heroin users and controls, and 454 DMSs between suicide completers and controls (p < 0.001. DMSs and corresponding genes (DMGs in heroin users showed significant differences in the preferential context of hyper and hypo DM. HyperDMSs were enriched in gene bodies and exons but depleted in promoters, whereas hypoDMSs were enriched in promoters and enhancers. In addition, hyperDMGs showed preference for genes expressed specifically by glutamatergic as opposed to GABAergic neurons and enrichment for axonogenesis- and synaptic-related gene ontology categories, whereas hypoDMGs were enriched for transcription factor activity- and gene expression regulation-related terms. Finally, we found that the DNAm-based “epigenetic age” of neurons from heroin users was younger than that in controls. Suicide-related results were more difficult to interpret. Collectively, these findings suggest that the observed DNAm differences could represent functionally significant marks of heroin-associated plasticity in the OFC.

  13. OPAL: Network for the Detection of Stratospheric Change ozone profiler assessment at Lauder, New Zealand 2. Intercomparison of revised results

    Science.gov (United States)

    McDermid, I. S.; Bergwerff, J. B.; Bodeker, G.; Boyd, I. S.; Brinksma, E. J.; Connor, B. J.; Farmer, R.; Gross, M. R.; Kimvilakani, P.; Matthews, W. A.; McGee, T. J.; Ormel, F. T.; Parrish, A.; Singh, U.; Swart, D. P. J.; Tsou, J. J.

    1998-11-01

    Following a blind intercomparison of ozone profiling instruments in the Network for the Detection of Stratospheric Change at Lauder, New Zealand, revisions to the analyses were made resulting in a new data set. This paper compares the revised results from two differential absorption lidars (RIVM and GSFC), a microwave radiometer (Millitech/LaRC), and electrochemical concentration cell (ECC) balloon sondes (NIWA). In general, the results are substantially improved compared to the earlier blind intercomparison. The level of agreement was similar both for single profiles and for the campaign average profile and was approximately 5% for the lidars and the sondes over the altitude range from 15 to 42 km (32 km for sondes). The revised microwave data show a bias of 5-10% high in the region from 22 to 42 km. Starting at 42 km, the lidar errors increase significantly, and comparisons of the microwave results were not possible above this altitude.

  14. Conventional physical therapy and physical therapy based on reflex stimulation showed similar results in children with myelomeningocele

    Directory of Open Access Journals (Sweden)

    Carolina Y. P. Aizawa

    Full Text Available ABSTRACT We aimed to investigate whether infants with myelomeningocele would improve their motor ability and functional independence after ten sessions of physical therapy and compare the outcomes of conventional physical therapy (CPT to a physical therapy program based on reflex stimulation (RPT. Twelve children were allocated to CPT (n = 6, age 18.3 months or RPT (n = 6, age 18.2 months. The RPT involved proprioceptive neuromuscular facilitation. Children were assessed with the Gross Motor Function Measure and the Pediatric Evaluation of Disability Inventory before and after treatment. Mann-Whitney tests compared the improvement on the two scales of CPT versus RPT and the Wilcoxon test compared CPT to RPT (before vs. after treatment. Possible correlations between the two scales were tested with Spearman correlation coefficients. Both groups showed improvement on self-care and mobility domains of both scales. There were no differences between the groups, before, or after intervention. The CPT and RPT showed similar results after ten weeks of treatment.

  15. Genomic and Enzymatic Results Show Bacillus cellulosilyticus Uses a Novel Set of LPXTA Carbohydrases to Hydrolyze Polysaccharides

    Science.gov (United States)

    Mead, David; Drinkwater, Colleen; Brumm, Phillip J.

    2013-01-01

    Background Alkaliphilic Bacillus species are intrinsically interesting due to the bioenergetic problems posed by growth at high pH and high salt. Three alkaline cellulases have been cloned, sequenced and expressed from Bacillus cellulosilyticus N-4 (Bcell) making it an excellent target for genomic sequencing and mining of biomass-degrading enzymes. Methodology/Principal Findings The genome of Bcell is a single chromosome of 4.7 Mb with no plasmids present and three large phage insertions. The most unusual feature of the genome is the presence of 23 LPXTA membrane anchor proteins; 17 of these are annotated as involved in polysaccharide degradation. These two values are significantly higher than seen in any other Bacillus species. This high number of membrane anchor proteins is seen only in pathogenic Gram-positive organisms such as Listeria monocytogenes or Staphylococcus aureus. Bcell also possesses four sortase D subfamily 4 enzymes that incorporate LPXTA-bearing proteins into the cell wall; three of these are closely related to each other and unique to Bcell. Cell fractionation and enzymatic assay of Bcell cultures show that the majority of polysaccharide degradation is associated with the cell wall LPXTA-enzymes, an unusual feature in Gram-positive aerobes. Genomic analysis and growth studies both strongly argue against Bcell being a truly cellulolytic organism, in spite of its name. Preliminary results suggest that fungal mycelia may be the natural substrate for this organism. Conclusions/Significance Bacillus cellulosilyticus N-4, in spite of its name, does not possess any of the genes necessary for crystalline cellulose degradation, demonstrating the risk of classifying microorganisms without the benefit of genomic analysis. Bcell is the first Gram-positive aerobic organism shown to use predominantly cell-bound, non-cellulosomal enzymes for polysaccharide degradation. The LPXTA-sortase system utilized by Bcell may have applications both in anchoring

  16. NMR metabolomics profiling of blood plasma mimics shows that medium- and long-chain fatty acids differently release metabolites from human serum albumin

    Science.gov (United States)

    Jupin, M.; Michiels, P. J.; Girard, F. C.; Spraul, M.; Wijmenga, S. S.

    2014-02-01

    Metabolite profiling by NMR of body fluids is increasingly used to successfully differentiate patients from healthy individuals. Metabolites and their concentrations are direct reporters of body biochemistry. However, in blood plasma the NMR-detected free-metabolite concentrations are also strongly affected by interactions with the abundant plasma proteins, which have as of yet not been considered much in metabolic profiling. We previously reported that many of the common NMR-detected metabolites in blood plasma bind to human serum albumin (HSA) and many are released by fatty acids present in fatted HSA. HSA is the most abundant plasma protein and main transporter of endogenous and exogenous metabolites. Here, we show by NMR how the two most common fatty acids (FAs) in blood plasma - the long-chain FA, stearate (C18:0) and medium-chain FA, myristate (C14:0) - affect metabolite-HSA interaction. Of the set of 18 common NMR-detected metabolites, many are released by stearate and/or myristate, lactate appearing the most strongly affected. Myristate, but not stearate, reduces HSA-binding of phenylalanine and pyruvate. Citrate signals were NMR invisible in the presence of HSA. Only at high myristate-HSA mole ratios 11:1, is citrate sufficiently released to be detected. Finally, we find that limited dilution of blood-plasma mimics releases HSA-bound metabolites, a finding confirmed in real blood plasma samples. Based on these findings, we provide recommendations for NMR experiments for quantitative metabolite profiling.

  17. Children of low socioeconomic status show accelerated linear growth in early childhood; results from the Generation R Study.

    Directory of Open Access Journals (Sweden)

    Lindsay M Silva

    Full Text Available OBJECTIVES: People of low socioeconomic status are shorter than those of high socioeconomic status. The first two years of life being critical for height development, we hypothesized that a low socioeconomic status is associated with a slower linear growth in early childhood. We studied maternal educational level (high, mid-high, mid-low, and low as a measure of socioeconomic status and its association with repeatedly measured height in children aged 0-2 years, and also examined to what extent known determinants of postnatal growth contribute to this association. METHODS: This study was based on data from 2972 mothers with a Dutch ethnicity, and their children participating in The Generation R Study, a population-based cohort study in Rotterdam, The Netherlands (participation rate 61%. All children were born between April 2002 and January 2006. Height was measured at 2 months (mid-90% range 1.0-3.9, 6 months (mid-90% range 5.6-11.4, 14 months (mid-90% range 13.7-17.9 and 25 months of age (mid-90% range 23.6-29.6. RESULTS: At 2 months, children in the lowest educational subgroup were shorter than those in the highest (difference: -0.87 cm; 95% CI: -1.16, -0.58. Between 1 and 18 months, they grew faster than their counterparts. By 14 months, children in the lowest educational subgroup were taller than those in the highest (difference at 14 months: 0.40 cm; 95% CI: 0.08,0.72. Adjustment for other determinants of postnatal growth did not explain the taller height. On the contrary, the differences became even larger (difference at 14 months: 0.61 cm; 95% CI: 0.26,0.95; and at 25 months: 1.00 cm; 95% CI: 0.57,1.43 CONCLUSIONS: Compared with children of high socioeconomic status, those of low socioeconomic status show an accelerated linear growth until the 18th month of life, leading to an overcompensation of their initial height deficit. The long-term consequences of these findings remain unclear and require further study.

  18. Malnutrition in Healthy Individuals Results in Increased Mixed Cytokine Profiles, Altered Neutrophil Subsets and Function.

    Science.gov (United States)

    Takele, Y; Adem, E; Getahun, M; Tajebe, F; Kiflie, A; Hailu, A; Raynes, J; Mengesha, B; Ayele, T A; Shkedy, Z; Lemma, M; Diro, E; Toulza, F; Modolell, M; Munder, M; Müller, I; Kropf, P

    2016-01-01

    Malnutrition is commonly associated with increased infectious disease susceptibility and severity. Whereas malnutrition might enhance the incidence of disease as well as its severity, active infection can in turn exacerbate malnutrition. Therefore, in a malnourished individual suffering from a severe infection, it is not possible to determine the contribution of the pre-existing malnutrition and/or the infection itself to increased disease severity. In the current study we focussed on two groups of malnourished, but otherwise healthy individuals: moderately malnourished (BMI: 18.4-16.5) and severely malnourished (BMI <16.5) and compared several immune parameters with those of individuals with a normal BMI (≥18.5). Our results show a similar haematological profile in all three groups, as well as a similar ratio of CD4+ and CD8+ T cells. We found significant correlations between low BMI and increased levels of T helper (Th) 1 (Interferon (IFN)-γ, (interleukin (IL)-2, IL-12), Th2 (IL-4, IL-5, IL-13), as well as IL-10, IL-33 and tumor necrosis factor-α, but not IL-8 or C reactive protein. The activities of arginase, an enzyme associated with immunosuppression, were similar in plasma, peripheral blood mononuclear cells (PBMC) and neutrophils from all groups and no differences in the expression levels of CD3ζ, a marker of T cell activation, were observed in CD4+ and CD8+T cells. Furthermore, whereas the capacity of neutrophils from the malnourished groups to phagocytose particles was not impaired, their capacity to produce reactive oxygen species was impaired. Finally we evaluated the frequency of a subpopulation of low-density neutrophils and show that they are significantly increased in the malnourished individuals. These differences were more pronounced in the severely malnourished group. In summary, our results show that even in the absence of apparent infections, healthy malnourished individuals display dysfunctional immune responses that might contribute to

  19. PROLONGED DUAL ANTIPLATELET THERAPY AFTER MYOCARDIAL INFARCTION: WHAT THE RESULTS OF THE PEGASUS-TIMI 54 TRIAL SHOW

    Directory of Open Access Journals (Sweden)

    I. S. Yavelov

    2017-01-01

    Full Text Available Prerequisites for the prolonged dual antiplatelet therapy after myocardial infarction are presented. The results of a large randomized placebo-controlled PEGASUS-TIMI 54 trial on the evaluation of efficacy and safety of prolonged use of a combination of acetylsalicylic acid and blocker P2Y12 platelet receptor, ticagrelor, in patients with high risk of thrombotic complications of atherosclerosis with a history of myocardial infarction 1-3 years ago are considered. The contribution of the results of this clinical trial in the modern understanding of the optimal use of antiplatelet agents in patients after myocardial infarction is analyzed.

  20. Gene expression profiles resulting from stable loss of p53 mirrors its role in tissue differentiation.

    Directory of Open Access Journals (Sweden)

    Oliver Couture

    Full Text Available The tumor suppressor gene p53 is involved in a variety of cellular activities such as cellular stress responses, cell cycle regulation and differentiation. In our previous studies we have shown p53's transcription activating role to be important in osteoblast differentiation. There is still a debate in the literature as to whether p53 inhibits or promotes differentiation. We have found p53 heterozygous mice to show a p53 dependency on some bone marker gene expression that is absent in knockout mice. Mice heterozygous for p53 also show a higher incidence of osteosarcomas than p53 knockout mice. This suggests that p53 is able to modify the environment within osteoblasts. In this study we compare changes in gene expression resulting after either a transient or stable reduction in p53. Accordingly we reduced p53 levels transiently and stably in C2C12 cells, which are capable of both myoblast and osteoblast differentiation, and compared the changes in gene expression of candidate genes regulated by the p53 pathway. Using a PCR array to assay for p53 target genes, we have found different expression profiles when comparing stable versus transient knockdown of p53. As expected, several genes with profound changes after transient p53 loss were related to apoptosis and cell cycle regulation. In contrast, stable p53 loss produced a greater change in MyoD and other transcription factors with tissue specific roles, suggesting that long term loss of p53 affects tissue homeostasis to a greater degree than changes resulting from acute loss of p53. These differences in gene expression were validated by measuring promoter activity of different pathway specific genes involved in differentiation. These studies suggest that an important role for p53 is context dependent, with a stable reduction in p53 expression affecting normal tissue physiology more than acute loss of p53.

  1. Host plant secondary metabolite profiling shows a complex, strain-dependent response of maize to plant growth-promoting rhizobacteria of the genus Azospirillum.

    Science.gov (United States)

    Walker, Vincent; Bertrand, Cédric; Bellvert, Floriant; Moënne-Loccoz, Yvan; Bally, René; Comte, Gilles

    2011-01-01

    Most Azospirillum plant growth-promoting rhizobacteria (PGPR) benefit plant growth through source effects related to free nitrogen fixation and/or phytohormone production, but little is known about their potential effects on plant physiology. These effects were assessed by comparing the early impacts of three Azospirillum inoculant strains on secondary metabolite profiles of two different maize (Zea mays) cultivars. After 10d of growth in nonsterile soil, maize methanolic extracts were analyzed by reverse-phase high-performance liquid chromatography (RP-HPLC) and secondary metabolites identified by liquid chromatography/mass spectrometry (LC/MS) and nuclear magnetic resonance (NMR). Seed inoculation resulted in increased shoot biomass (and also root biomass with one strain) of hybrid PR37Y15 but had no stimulatory effect on hybrid DK315. In parallel, Azospirillum inoculation led to major qualitative and quantitative modifications of the contents of secondary metabolites, especially benzoxazinoids, in the maize plants. These modifications depended on the PGPR strain×plant cultivar combination. Thus, Azospirillum inoculation resulted in early, strain-dependent modifications in the biosynthetic pathways of benzoxazine derivatives in maize in compatible interactions. This is the first study documenting a PGPR effect on plant secondary metabolite profiles, and suggests the establishment of complex interactions between Azospirillum PGPR and maize. © The Authors (2010). Journal compilation © New Phytologist Trust (2010).

  2. Early Results Show Reduced Infection Rate Using No-touch Technique for Expander/ADM Breast Reconstruction

    Directory of Open Access Journals (Sweden)

    Henry B. Wilson, MD, FACS

    2015-03-01

    Full Text Available Summary: Infection is a common complication of immediate breast reconstruction that often leads to device removal, a result emotionally devastating to the patient and frustrating for her surgeon. “No-touch” techniques have been used in other surgical disciplines and plastic surgery, but they have not been reported for breast reconstruction with tissue expanders or implants and acellular dermis. We report a novel technique of tissue expander and acellular dermis placement using no-touch principles with a self-retaining retractor system that holds promise to decrease infectious complications of breast reconstruction.

  3. Norwegian lakes show widespread recovery from acidification; results from national surveys of lakewater chemistry 1986-1997

    Directory of Open Access Journals (Sweden)

    B. L. Skjelkvåle

    1998-01-01

    Full Text Available Surveys of 485 lakes in Norway conducted in 1986 and again in 1995 reveal widespread chemical recovery from acidification. Sulphate concentrations in lakes have decreased by 40% in acidified areas in southern Norway. This decrease has been compensated about 25% by decreases in concentrations of base cations and of 75% by increased Acid Neutralising Capacity (ANC. The increased ANC in turn reflects lower concentrations of acidic cations Aluminum (ALn+ and Hydrogen (H+. A sub-set of 78 of the 485 lakes sampled yearly between 1986 and 1997 shows that, at first most of the decrease in non-marine sulphate (SO4* was compensated by a decrease in base cations, such that ANC remained unchanged. Then as SO4* continued to decrease, the concentrations of non- marine calcium and magnesium ((Ca+Mg* levelled out. Consequently, ANC increased, and H+ and Aln+ started to decrease. In eastern Norway, this shift occurred in 1989–90, and came slightly later in southern and western Norway. Similar shifts in trends in about 1991–92 can also be seen in the non-acidified areas in central and northern Norway. This shift in trends is not as pronounced in western Norway, perhaps because of the confounding influence of sea-salt episodes on water chemistry. This is the first documented national-scale recovery from acidification due to reduced acid deposition. Future climate warming and potentially increased N-leaching can counteract the positive trends in recovery from acidification.

  4. Effect of dactyloscopic powders on DNA profiling from enhanced fingerprints: results from an experimental study.

    Science.gov (United States)

    Tozzo, Pamela; Giuliodori, Alice; Rodriguez, Daniele; Caenazzo, Luciana

    2014-03-01

    We conducted a study on the effect of fingerprint enhancement methods on subsequent short tandem repeat profiling. First, we performed a study typing blood traces deposited on 5 different surfaces, treated with 8 types of dactyloscopic powders. Three different DNA extraction methods were used. Subsequently, we analyzed latent fingerprints on the same 5 surfaces enhanced with the 8 different powders used in the first part of the study. This study has demonstrated that DNA profiling can be performed on fingerprints left on different substrates, and the substrate will affect the amount of DNA that can be recovered for DNA typing. In the first phase of the study, a profile was obtained in 92% of the 120 samples analyzed; in the second part, in 55% of the 80 samples analyzed, we obtained a profile complete in 32.5% of the cases. From the results obtained, it seems that the powders used in latent fingerprints enhancement, rather than having a direct inhibitory effect on extraction and amplification of DNA, may cause partial degradation of DNA, reducing the efficiency of amplification reaction. It should not be forgotten that these results were obtained under laboratory conditions, and in real caseworks, there may still be different problems involved.

  5. Bilateral femoral neck fractures resulting from pregnancy-associated osteoporosis showed bone marrow edema on magnetic resonance imaging.

    Science.gov (United States)

    Kasahara, Kyoko; Kita, Nobuyuki; Kawasaki, Taku; Morisaki, Shinsuke; Yomo, Hiroko; Murakami, Takashi

    2017-06-01

    Femoral neck fractures resulting from pregnancy-associated osteoporosis is a rare condition. Herein, we report an undoubted case of pregnancy-associated osteoporosis in a 38-year-old primiparous patient with pre-existing anorexia nervosa who suffered bilateral femoral neck fractures in the third trimester and early post-partum period. Magnetic resonance imaging revealed femoral neck fractures as well as diffuse marrow edema involving both femoral heads, which are considered under ordinary circumstances as characteristic imaging findings of transient osteoporosis of the hip. Based on our experience, we propose that pregnancy-associated osteoporosis might be present in femoral neck fractures attributed to transient osteoporosis of the hip in pregnancy. Conversely, bone status should be carefully and accurately estimated in cases of potential transient osteoporosis of the hip in pregnancy to reduce future fracture risk. © 2017 The Authors Journal of Obstetrics and Gynaecology Research published by John Wiley & Sons Australia, Ltd on behalf of Japan Society of Obstetrics and Gynecology.

  6. First Experimental Results And Improvements On Profile Measurements With The Vibrating Wire Scanner

    CERN Document Server

    Arutunian, S G; Dobrovolski, N M; Mailian, M R; Soghoyan, H E; Vasiniuk, I E

    2003-01-01

    The paper presents the first experimental results of transverse profile scans using a wire scanner based on a vibrating wire (vibrating wire scanner - VWS). The measurements were performed at the injector electron beam (6 nA) of the Yerevan synchrotron. The beam profile information is obtained by measuring the wire natural oscillations that depend on the wire temperature. This first experiments on weak electron beam proved this new method as a very sensitive tool, even suitable for tail measurements. Additional, improvements were tested to overcome some problems connected with signal conditioning and signal transfer in the presence of electromagnetic noise. As a result the noises were neatly separated and reduced. A mathematical method for rejection of distorted data was developed. Experiments with the scanner at the PETRA accelerator at DESY are planned for measurements of beam tails.

  7. Results of the NASA Kennedy Space Center 50-MHz Doppler Radar Wind Profiler Operational Acceptance Test

    Science.gov (United States)

    Barbre', Robert E., Jr.; Decker, Ryan K.; Leahy, Frank B.; Huddleston, Lisa

    2016-01-01

    This paper presents results of the new Kennedy Space Center (KSC) 50-MHz Doppler Radar Wind Profiler (DRWP) Operational Acceptance Test (OAT). The goal of the OAT was to verify the data quality of the new DRWP against the performance of the previous DRWP in order to use wind data derived by the new DRWP for space launch vehicle operations support at the Eastern Range. The previous DRWP was used as a situational awareness asset for mission operations to identify rapid changes in the wind environment that weather balloons cannot depict. The Marshall Space Flight Center's Natural Environments Branch assessed data from the new DRWP collected during Jan-Feb 2015 against a specified set of test criteria. Data examination verified that the DRWP provides complete profiles every five minutes from 1.8-19.5 km in vertical increments of 150 m. Analysis of 49 concurrent DRWP and balloon profiles presented root mean square wind component differences around 2.0 m/s. Evaluation of the DRWP's coherence between five-minute wind pairs found the effective vertical resolution to be Nyquist-limited at 300 m for both wind components. In addition, the sensitivity to rejecting data that do not have adequate signal was quantified. This paper documents the data, quality control procedures, methodology, and results of each analysis.

  8. Tailoring the near-surface composition profiles of pressure-sensitive adhesive films and the resulting mechanical properties.

    Science.gov (United States)

    Diethert, Alexander; Ecker, Katharina; Peykova, Yana; Willenbacher, Norbert; Müller-Buschbaum, Peter

    2011-06-01

    We present a possibility of tailoring the near-surface composition profiles of pressure sensitive adhesive (PSA) films by an exposure to atmospheres of different relative humidities (RHs). The statistical copolymer P(EHA-stat-20MMA) with a majority of ethylhexylacrylate (EHA) and a minority of methylmethacrylate (MMA), being cast from a toluene based solution, is chosen as a model system. The near-surface composition profile is probed with X-ray reflectivity. All probed samples show an enrichment of PMMA at the sample surface; however, the near-surface PMMA content strongly increases with increasing RH. The influence of the RH on the composition profile is present down to a depth of 50 nm. Therefore the surface tensions being derived from contact angle measurements do not show any measurable humidity dependence. In contrast, in a mechanical tack test with a smooth punch surface, a strong influence is probed. This observation can be explained by considering the integrated PMMA content over an appropriate near-surface region and the resulting impact on the cavitation process. © 2011 American Chemical Society

  9. Job Profiles of Biomedical Informatics Graduates. Results of a Graduate Survey.

    Science.gov (United States)

    Ammenwerth, E; Hackl, W O

    2015-01-01

    Biomedical informatics programs exist in many countries. Some analyses of the skills needed and of recommendations for curricular content for such programs have been published. However, not much is known of the job profiles and job careers of their graduates. To analyse the job profiles and job careers of 175 graduates of the biomedical informatics bachelor and master program of the Tyrolean university UMIT. Survey of all biomedical informatics students who graduated from UMIT between 2001 and 2013. Information is available for 170 graduates. Eight percent of graduates are male. Of all bachelor graduates, 86% started a master program. Of all master graduates, 36% started a PhD. The job profiles are quite diverse: at the time of the survey, 35% of all master graduates worked in the health IT industry, 24% at research institutions, 9% in hospitals, 9% as medical doctors, 17% as informaticians outside the health care sector, and 6% in other areas. Overall, 68% of the graduates are working as biomedical informaticians. The results of the survey indicate a good job situation for the graduates. The job opportunities for biomedical informaticians who graduated with a bachelor or master degree from UMIT seem to be quite good. The majority of graduates are working as biomedical informaticians. A larger number of comparable surveys of graduates from other biomedical informatics programs would help to enhance our knowledge about careers in biomedical informatics.

  10. ASGR1 and ASGR2, the Genes that Encode the Asialoglycoprotein Receptor (Ashwell Receptor, Are Expressed in Peripheral Blood Monocytes and Show Interindividual Differences in Transcript Profile

    Directory of Open Access Journals (Sweden)

    Rebecca Louise Harris

    2012-01-01

    Full Text Available Background. The asialoglycoprotein receptor (ASGPR is a hepatic receptor that mediates removal of potentially hazardous glycoconjugates from blood in health and disease. The receptor comprises two proteins, asialoglycoprotein receptor 1 and 2 (ASGR1 and ASGR2, encoded by the genes ASGR1 and ASGR2. Design and Methods. Using reverse transcription amplification (RT-PCR, expression of ASGR1 and ASGR2 was investigated in human peripheral blood monocytes. Results. Monocytes were found to express ASGR1 and ASGR2 transcripts. Correctly spliced transcript variants encoding different isoforms of ASGR1 and ASGR2 were present in monocytes. The profile of transcript variants from both ASGR1 and ASGR2 differed among individuals. Transcript expression levels were compared with the hepatocyte cell line HepG2 which produces high levels of ASGPR. Monocyte transcripts were 4 to 6 orders of magnitude less than in HepG2 but nonetheless readily detectable using standard RT-PCR. The monocyte cell line THP1 gave similar results to monocytes harvested from peripheral blood, indicating it may provide a suitable model system for studying ASGPR function in this cell type. Conclusions. Monocytes transcribe and correctly process transcripts encoding the constituent proteins of the ASGPR. Monocytes may therefore represent a mobile pool of the receptor, capable of reaching sites remote from the liver.

  11. Conversion of cDNA differential display results (DDRT-PCR) into quantitative transcription profiles

    Science.gov (United States)

    Venkatesh, Balakrishnan; Hettwer, Ursula; Koopmann, Birger; Karlovsky, Petr

    2005-01-01

    Background Gene expression studies on non-model organisms require open-end strategies for transcription profiling. Gel-based analysis of cDNA fragments allows to detect alterations in gene expression for genes which have neither been sequenced yet nor are available in cDNA libraries. Commonly used protocols for gel-based transcript profiling are cDNA differential display (DDRT-PCR) and cDNA-AFLP. Both methods have been used merely as qualitative gene discovery tools so far. Results We developed procedures for the conversion of cDNA Differential Display data into quantitative transcription profiles. Amplified cDNA fragments are separated on a DNA sequencer and detector signals are converted into virtual gel images suitable for semi-automatic analysis. Data processing consists of four steps: (i) cDNA bands in lanes corresponding to samples treated with the same primer combination are matched in order to identify fragments originating from the same transcript, (ii) intensity of bands is determined by densitometry, (iii) densitometric values are normalized, and (iv) intensity ratio is calculated for each pair of corresponding bands. Transcription profiles are represented by sets of intensity ratios (control vs. treatment) for cDNA fragments defined by primer combination and DNA mobility. We demonstrated the procedure by analyzing DDRT-PCR data on the effect of secondary metabolites of oilseed rape Brassica napus on the transcriptome of the pathogenic fungus Leptosphaeria maculans. Conclusion We developed a data processing procedure for the quantitative analysis of amplified cDNA fragments separated by electrophoresis. The system utilizes common software and provides an open-end alternative to DNA microarray analysis of the transcriptome. It is expected to work equally well with DDRT-PCR and cDNA-AFLP data and be useful particularly in reseach on organisms for which microarray analysis is not available or economical. PMID:15807902

  12. Pea fiber and wheat bran fiber show distinct metabolic profiles in rats as investigated by a 1H NMR-based metabolomic approach.

    Directory of Open Access Journals (Sweden)

    Guangmang Liu

    Full Text Available This study aimed to examine the effect of pea fiber (PF and wheat bran fiber (WF supplementation in rat metabolism. Rats were assigned randomly to one of three dietary groups and were given a basal diet containing 15% PF, 15% WF, or no supplemental fiber. Urine and plasma samples were analyzed by NMR-based metabolomics. PF significantly increased the plasma levels of 3-hydroxybutyrate, and myo-inositol as well as the urine levels of alanine, hydroxyphenylacetate, phenylacetyglycine, and α-ketoglutarate. However, PF significantly decreased the plasma levels of isoleucine, leucine, lactate, and pyruvate as well as the urine levels of allantoin, bile acids, and trigonelline. WF significantly increased the plasma levels of acetone, isobutyrate, lactate, myo-inositol, and lipids as well as the urine levels of alanine, lactate, dimethylglycine, N-methylniconamide, and α-ketoglutarate. However, WF significantly decreased the plasma levels of amino acids, and glucose as well as the urine levels of acetate, allantoin, citrate, creatine, hippurate, hydroxyphenylacetate, and trigonelline. Results suggest that PF and WF exposure can promote antioxidant activity and can exhibit common systemic metabolic changes, including lipid metabolism, energy metabolism, glycogenolysis and glycolysis metabolism, protein biosynthesis, and gut microbiota metabolism. PF can also decrease bile acid metabolism. These findings indicate that different fiber diet may cause differences in the biofluid profile in rats.

  13. Chia Seed Shows Good Protein Quality, Hypoglycemic Effect and Improves the Lipid Profile and Liver and Intestinal Morphology of Wistar Rats.

    Science.gov (United States)

    da Silva, Bárbara Pereira; Dias, Desirrê Morais; de Castro Moreira, Maria Eliza; Toledo, Renata Celi Lopes; da Matta, Sérgio Luis Pinto; Lucia, Ceres Mattos Della; Martino, Hércia Stampini Duarte; Pinheiro-Sant'Ana, Helena Maria

    2016-09-01

    Chia has been consumed by the world population due to its high fiber, lipids and proteins content. The objective was to evaluate the protein quality of chia untreated (seed and flour) and heat treated (90 °C/20 min), their influence on glucose and lipid homeostasis and integrity of liver and intestinal morphology of Wistar rats. 36 male rats, weanling, divided into six groups which received control diet (casein), free protein diet (aproteic) and four diet tests (chia seed; chia seed with heat treatment; chia flour and chia flour with heat treatment) for 14 days were used. The protein efficiency ratio (PER), net protein ratio (NPR) and true digestibility (TD) were evaluated. The biochemical variables and liver and intestinal morphologies of animals were determined. The values of PER, NPR and TD did not differ among the animals that were fed with chia and were lower than the control group. The animals that were fed with chia showed lower concentrations of glucose; triacylglycerides, low-density lipoprotein cholesterol and very low-density lipoprotein and higher high-density lipoprotein cholesterol than the control group. The liver weight of animals that were fed with chia was lower than the control group. Crypt depth and thickness of intestinal muscle layers were higher in groups that were fed with chia. The consumption of chia has shown good digestibility, hypoglycemic effect, improved lipid and glycemic profiles and reduced fat deposition in liver of animals, and also promoted changes in intestinal tissue that enhanced its functionality.

  14. Leaf metabolite profile of the Brazilian resurrection plant Barbacenia purpurea Hook. (Velloziaceae shows two time-dependent responses during desiccation and recovering

    Directory of Open Access Journals (Sweden)

    Vanessa Fuentes Suguiyama

    2014-03-01

    Full Text Available Barbacenia purpurea is a resurrection species endemic to rock outcrops, in Rio de Janeiro, Brazil. It tolerates great temperature variations, which are associated to periods of up to 30 days without precipitation. Using a metabolomic approach, we analyzed, under winter and summer conditions, changes in the leaf metabolite profile (MP of potted plants of B. purpurea submitted to daily watered and water deficit for at least 20 days and subsequent slow rehydration for 5 days. Leaves were collected at different time points and had their MP analyzed by GC/MS, HPAEC, and UHPLC techniques, allowing the identification of more than 60 different compounds, including organic and amino acids, sugars, and polyols, among others. In the winter experiment, results suggest the presence of two time-dependent responses in B. purpurea under water stress. The first one starts with the increase in the content of caffeoyl-quinic acids, substances with strong antioxidant activity, until the 16th day of water suppression. When RWC reached less than 80% and 70%, in winter and summer respectively, it was observed an increase in polyols and monosaccharides, followed by an increment in the content of RFO, suggesting osmotic adjustment. Amino acids, such as GABA and asparagine, also increased due to 16 days of water suppression. During rehydration, the levels of the mentioned compounds became similar to those found at the beginning of the experiment and when compared to daily watered plants. We conclude that the tolerance of B. purpurea to dehydration involves the perception of water deficit intensity, which seems to result in different strategies to overcome the gradient of water availability imposed along a certain period of stress mainly during winter. Data from summer experiment indicate that the metabolism of B. pupurea was already primed for drought stress. The accumulation of phenolics in summer seemed to be more temperature and irradiance-dependent than on the RWC.

  15. Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth.

    Science.gov (United States)

    Kirkegaard, Kirstine; Villesen, Palle; Jensen, Jacob Malte; Hindkjær, Johnny Juhl; Kølvraa, Steen; Ingerslev, Hans Jakob; Lykke-Hartmann, Karin

    2015-10-25

    Results from animal models points towards the existence of a gene expression profile that is distinguishably different in viable embryos compared with non-viable embryos. Knowledge of human embryo transcripts is however limited, in particular with regard to how gene expression is related to clinical outcome. The purpose of the present study was therefore to determine the global gene expression profiles of human blastocysts. Next Generation Sequencing was used to identify genes that were differentially expressed in non-implanted embryos and embryos resulting in live birth. Three trophectoderm biopsies were obtained from morphologically high quality blastocysts resulting in live birth and three biopsies were obtained from non-implanting blastocysts of a comparable morphology. Total RNA was extracted from all samples followed by complete transcriptome sequencing. Using a set of filtering criteria, we obtained a list of 181 genes that were differentially expressed between trophectoderm biopsies from embryos resulting in either live birth or no implantation (negative hCG), respectively. We found that 37 of the 181 genes displayed significantly differential expression (plive birth and non-implanting, respectively. Out of the 181 genes, almost 80% (145 genes) were up-regulated in biopsies from un-implanted embryos, whereas only 20% (36 genes) showed an up-regulation in the samples from embryos resulting in live birth. Our findings suggest the presence of molecular differences visually undetectable between implanted and non-implanted embryos, and represent a proof of principle study. Copyright © 2015 Elsevier B.V. All rights reserved.

  16. Multiple intravenous doses of paracetamol result in a predictable pharmacokinetic profile in very preterm infants.

    Science.gov (United States)

    van Ganzewinkel, C; Derijks, L; Anand, K J S; van Lingen, R A; Neef, C; Kramer, B W; Andriessen, P

    2014-06-01

    The therapeutic options available to treat neonatal pain are limited, and one alternative for nonopioid systemic treatment is paracetamol. However, pharmacokinetic data from prolonged administration of intravenous paracetamol in neonates are limited. The aim of this study was to present pharmacokinetics after multiple dose of intravenous paracetamol in very preterm infants of paracetamol (7.5 mg/kg). Blood samples were taken to measure paracetamol, glutathione and hepatic function, together with urine samples for paracetamol metabolites. A two-compartment pharmacokinetic model gave the best fit for all individual patients and resulted in a predictable pharmacokinetic profile. The estimated pharmacokinetic population parameters were volume of distribution 0.764 ± 0.225 L/kg, elimination rate constant (ke ) 0.117 ± 0.091/h and intercompartment rate constants k12 0.607 ± 0.734/h and k21 1.105 ± 0.762/h. Our study found that multiple doses of intravenous paracetamol resulted in a predictable pharmacokinetic profile in very preterm infants. Increases in postmenstrual age and weight were associated with increased clearance. No evidence of hepatotoxicity was found. ©2014 Foundation Acta Paediatrica. Published by John Wiley & Sons Ltd.

  17. A Subgroup of First-Degree Relatives of Crohn's Disease Patients Shows a Profile of Inflammatory Markers in the Blood Which is More Typical of Crohn's Disease Patients Than of Normal Individuals

    Directory of Open Access Journals (Sweden)

    2006-01-01

    Full Text Available Introduction. Family member with IBD is the greatest risk factor for developing the disease. The hematological profileof first-degree relatives (FDRs of Crohn's disease (CD patients was studied in order to identify healthy FDRs at risk to develop disease. Materials and methods. Thirty CD patients, 90 FDRs, and 28 non-related individuals (controls were enrolled. Hematological profile and C-reactive protein were determined. Results. All hematological parameters were significantly different in CD patients compared to controls, with significantly higher levels of CRP, WBC, PMN, MONO, and PLT and lower Hb and lymphocyte count. The hematological profile of FDRs showed values between the controls and CD patients with ten FDRs that their parameters matched those of CD patients and significantly different from other FDRs. This group was defined as high-risk relatives (HRRs. Conclusions. Analysis of the hematological profile and CRP level might be proven as a fast, reliable, and less money-consuming tool to identify FDRs with a probable increased risk to develop the disease.

  18. Commissioning and First Results of the Electron Beam Profiler in the Main Injector at Fermilab

    Energy Technology Data Exchange (ETDEWEB)

    Thurman-Keup, R. [Fermilab; Alvarez, M. [Fermilab; Fitzgerald, J. [Fermilab; Lundberg, C. [Fermilab; Prieto, P. [Fermilab; Zagel, J. [Fermilab; Blokland, W. [Oak Ridge

    2017-08-01

    The planned neutrino program at Fermilab requires large proton beam intensities in excess of 2 MW. Measuring the transverse profiles of these high intensity beams is challenging and often depends on non-invasive techniques. One such technique involves measuring the deflection of a probe beam of electrons with a trajectory perpendicular to the proton beam. A device such as this is already in use at the Spallation Neutron Source at ORNL and a similar device has been installed in the Main Injector at Fermilab. Commissioning of the device is in progress with the goal of having it operational by the end of the year. The status of the commissioning and initial results will be presented

  19. Profile control simulations and experiments on TCV: a controller test environment and results using a model-based predictive controller

    Science.gov (United States)

    Maljaars, E.; Felici, F.; Blanken, T. C.; Galperti, C.; Sauter, O.; de Baar, M. R.; Carpanese, F.; Goodman, T. P.; Kim, D.; Kim, S. H.; Kong, M.; Mavkov, B.; Merle, A.; Moret, J. M.; Nouailletas, R.; Scheffer, M.; Teplukhina, A. A.; Vu, N. M. T.; The EUROfusion MST1-team; The TCV-team

    2017-12-01

    The successful performance of a model predictive profile controller is demonstrated in simulations and experiments on the TCV tokamak, employing a profile controller test environment. Stable high-performance tokamak operation in hybrid and advanced plasma scenarios requires control over the safety factor profile (q-profile) and kinetic plasma parameters such as the plasma beta. This demands to establish reliable profile control routines in presently operational tokamaks. We present a model predictive profile controller that controls the q-profile and plasma beta using power requests to two clusters of gyrotrons and the plasma current request. The performance of the controller is analyzed in both simulation and TCV L-mode discharges where successful tracking of the estimated inverse q-profile as well as plasma beta is demonstrated under uncertain plasma conditions and the presence of disturbances. The controller exploits the knowledge of the time-varying actuator limits in the actuator input calculation itself such that fast transitions between targets are achieved without overshoot. A software environment is employed to prepare and test this and three other profile controllers in parallel in simulations and experiments on TCV. This set of tools includes the rapid plasma transport simulator RAPTOR and various algorithms to reconstruct the plasma equilibrium and plasma profiles by merging the available measurements with model-based predictions. In this work the estimated q-profile is merely based on RAPTOR model predictions due to the absence of internal current density measurements in TCV. These results encourage to further exploit model predictive profile control in experiments on TCV and other (future) tokamaks.

  20. Processing and preliminary results of NFP/PNR20 seismic reflection profiles from the Western Swiss Alps

    Science.gov (United States)

    Levato, L.; Pruniaux, B.; Burri, M.; Escher, A.; Olivier, R.; Sellami, S.; Wagner, J.-J.

    1993-03-01

    Results of near-vertical seismic reflection profiling conducted in the western Swiss Alps are presented. All profiles were recorded using both high-fold Vibroseis and high-energy but low-fold dynamite sources, yielding two independent data sets. The Vibroseis seismic sections provide markedly superior resolution of upper crustal units down to the top of the crystalline basement. On these sections, high-impedance contrasts within the nappes are the source of strong reflections, and the basal thrust of the Penninic nappes (Penninic front) delineates a lateral change in reflection character. By comparison, the dynamite sections provide generally higher-quality images of the deep crust. In particular, they show prominent bands of strong reflections in the lower crust and a well-defined reflection Moho deepening from ~ 12 s (about 36 km) in the north to ~ 16 s (about 48 km) in the south. It is concluded that both types of data are required to resolve accurately shallow and deep structures in the Swiss Alps. Preliminary results of the dynamite survey from the most recently recorded line support proposals that shortening of the basement in the external domain has occurred, in agreement with interpretations of the ECORS-CROP seismic reflection line further south.

  1. Profile of oral piercing users and implications resulting from its use

    Directory of Open Access Journals (Sweden)

    Jeferson da Costa Marchiori

    2009-01-01

    Full Text Available Objective: To identify the profile of oral piercing users and the possible implications resulting from its use, as well as explaining to dentists about the possible consequences to their patients as a result of wearing this type of jewelry in the oral region.Methods: Fifty-one oral piercing users were interviewed. They were aged between 18 and 42 year, from different social classes, and resident in the city of Santa Maria, Rio Grande do Sul, Brazil.Results: Based on the questionnaires analyzed, the majority of users had tongue or lip piercing as a way to express identity, and 45% reported the occurrence of some type of oral and/or general alteration associated with the use of piercings. Conclusion: The majority of alterations are harmful to the piercing user’s health, therefore, professionals in the Dentistry area must be warnedto explain to their patients, who use oral piercings, about the consequences that may occur and the need for special care to ameliorate these problems.

  2. mRNA profiling for the identification of blood-Results of a collaborative EDNAP exercise

    DEFF Research Database (Denmark)

    Haas, Cordula; Hanson, E; Bär, W

    2010-01-01

    A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, usi...

  3. mRNA profiling for the identification of blood--Results of a collaborative EDNAP exercise

    DEFF Research Database (Denmark)

    Haas, C.; Hanson, E.; Bär, W.

    2011-01-01

    A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, usi...

  4. Giant cell glioblastoma and pleomorphic xanthoastrocytoma show different immunohistochemical profiles for neuronal antigens and p53 but share reactivity for class III beta-tubulin.

    Science.gov (United States)

    Martinez-Diaz, Hilda; Kleinschmidt-DeMasters, B K; Powell, Suzanne Z; Yachnis, Anthony T

    2003-09-01

    Giant cell glioblastoma multiforme (GCGBM) and pleomorphic xanthoastrocytoma (PXA) are clinically, radiographically, and histologically distinct tumors of the central nervous system. However, they share features of gross circumscription, reticulin deposition, lymphocytic infiltrates, and prominent populations of tumor giant cells. Neuronal antigens have been detected in the neoplastic cells of PXAs, but to our knowledge have not been studied previously in GCGBMs. While TP53 is mutated in most GCGBMs, a feature usually paralleled by strong immunostaining of the protein, the expression pattern of PXAs has not been extensively studied. To compare the immunoprofiles of GCGBM and PXA with regard to neuronal antigens and p53 and to evaluate the potential diagnostic utility of such a panel. Archival paraffin sections of 9 GCGBMs and 9 PXAs were immunostained for class III beta-tubulin, neuronal nuclear antigen, neurofilament protein, synaptophysin, glial fibrillary acidic protein, and p53. Giant cell glioblastomas were strongly immunoreactive for class III beta-tubulin and glial fibrillary acidic protein, but showed only rare staining for the other neuronal polypeptides. In contrast, PXAs usually showed at least focal staining of individual tumor cells for most of the neuronal antigens tested. Tubulin was strongly positive in tumor giant cells and in smaller neoplastic cells of both tumor types. Double-immunolabeling revealed distinct populations of tumor cells that expressed either glial fibrillary acidic protein or tubulin and dual-labeling of individual cells in GCGBM and PXA. Strong p53 staining was observed in many tumor cells in 5 of 8 GCGBMs tested, while staining for this antigen was negative or focally positive in 6 of 8 PXAs examined. Giant cell glioblastoma multiforme and PXA show distinct patterns of immunoreactivity for neuronal antigens and p53 that may be useful diagnostically in difficult cases or in limited samples. These results provide further evidence

  5. Aqueous and lipid nuclear magnetic resonance metabolomic profiles of the earthworm Aporrectodea caliginosa show potential as an indicator species for environmental metabolomics.

    Science.gov (United States)

    Brown, Jeffrey N; Samuelsson, Linda; Bernardi, Giuliana; Gooneratne, Ravi; Larsson, D G Joakim

    2014-10-01

    The common pasture earthworm Aporrectodea caliginosa has often been neglected in environmental metabolomics in favor of species easily bred in the laboratory. The present study assigns aqueous metabolites in A. caliginosa using high-resolution 1- and 2-dimensional nuclear magnetic resonance (NMR) spectroscopy. In total, 51 aqueous metabolites were identified, including typical amino acids (alanine, leucine, asparagine, phenylalanine), sugars (maltose, glucose), the dominant earthworm-specific 2-hexyl-5-ethyl-furansulfonate, and several previously unreported metabolites (oxoglutarate, putrescine). Examining the lesser-known earthworm lipid metabolome showed various lipid fatty acyl chains, cholesterol, and phosphatidylcholine. To briefly test if the NMR metabolomic techniques could differentiate A. caliginosa from different sites, earthworms were collected from 2 adjacent farms. Orthogonal partial least squares discriminant analysis detected metabolomic differences, suggesting the worms from the 2 sites differed in their energy metabolism, as indicated by altered levels of alanine, glutamine, glutamate, malate, fumarate, and lipids. Evidence of greater utilization of lipid energy reserves and onset of protein catabolism was also present. While the precise cause of the metabolomic differences could not be determined, the results show the potential of this species for further environmental metabolomic studies. © 2014 SETAC.

  6. Profile and results of frail patient assessed by advanced practice nursing in an Emergency Department.

    Science.gov (United States)

    Solé-Casals, Montserrat; Chirveches-Pérez, Emilia; Puigoriol-Juvanteny, Emma; Nubó-Puntí, Núria; Chabrera-Sanz, Carolina; Subirana-Casacuberta, Mireia

    2017-06-02

    To describe the profile of patients evaluated by Nurse Care Management in an Emergency Department and identify the type of alternative healthcare resource assigned and report the results of clinical practice. Prospective follow-up, on admission to the Emergency Department in an acute hospital and on discharge from the alternative healthcare resource, of patients assessed by Nurse Care Management, from July to December 2015. The patient characteristics, social environment and results of clinical practice were studied. 190 patients were included of whom 13 were readmitted (6.8%). 122 (59.8%) cases from the Emergency Department were referred to to intermediate care facilities, 71 (34.8%) cases for domiciliary care, 10 (4.9%) cases were referred to an acute care hospital and 1 (0.5%) died. Patients referred to intermediate care were more complex, presented geriatric syndromes as their reason for admission and diagnosed with dementia, while those referred to home care presented more respiratory and cardiovascular illnesses (p <0.05). The mean Barthel Index and polypharmacy before emergency admission were higher than at the time of discharge from the alternative healthcare resource (p <0.05). Patients presenting with advanced age, complexity, comorbidity, are referred to intermediate care facilities or domiciliary care, they are admitted to acute care hospitasl and are readmitted less than other patients. After being discharged from the alternative resource, they lose functional capacity and present less polypharmacy. Copyright © 2017 Elsevier España, S.L.U. All rights reserved.

  7. Different gene expression profiles in normo- and dyslipidemic men after fish oil supplementation: results from a randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Schmidt Simone

    2012-08-01

    Full Text Available Abstract Background Epidemiological studies have suggested the benefits of omega-3 polyunsaturated fatty acids (n-3 PUFAs on cardiovascular health, but only limited data are available describing n-3 PUFA regulated pathways in humans. The aim of this study was to investigate the effects of n-3 PUFA administration on whole genome expression profiles in the blood of normo- and dyslipidemic subjects. Methods Differentially expressed genes were detected after four hours, one week and twelve weeks of supplementation with either fish oil (FO or corn oil in normo- and dyslipidemic men using whole genome microarrays. Results Independent of the oil, a significantly higher number of genes was regulated in dyslipidemic subjects compared to normolipidemic subjects. Pathway analyses discovered metabolisms dominantly affected by FO after twelve weeks of supplementation, including the lipid metabolism, immune system and cardiovascular diseases. Several pro-inflammatory genes, in particular, were down-regulated in dyslipidemic subjects, indicating the immune-modulatory and anti-inflammatory capability of FO and its bioactive FAs, eicosapentaenoic acid and docosahexaenoic acid. Conclusions This is the first study showing significant differences in gene expression profiles between normo- and dyslipidemic men after FO supplementation. Further studies need to clarify the exact role of n-3 PUFAs in pathways and metabolisms which were identified as being regulated after FO supplementation in this study. Trial registration ClinicalTrials.gov (ID: NCT01089231

  8. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi.

    Science.gov (United States)

    Peña-Espinoza, Miguel; Boas, Ulrik; Williams, Andrew R; Thamsborg, Stig M; Simonsen, Henrik T; Enemark, Heidi L

    2015-12-01

    The study investigated direct anthelmintic effects of sesquiterpene lactones (SL)-containing extracts from forage chicory against free-living and parasitic stages of Ostertagia ostertagi. Freeze-dried leaves from chicory cultivars 'Spadona' and 'Puna II' were extracted using methanol/water. Total SL were further fractionated by solid-phase extraction and resulting extracts were characterised by high-performance liquid chromatography (HPLC). O. ostertagi eggs from faeces of mono-infected calves were hatched and L1 were used in a larval feeding inhibition assay (LFIA), while cultured L3 were used in a larval exsheathment inhibition assay (LEIA). Adult worms were immediately recovered after slaughter and used for motility inhibition assays (AMIA). Electron microscopy (EM) was performed on adult O. ostertagi exposed to 1000 μg extract mL(-1) of both chicory cultivars. In all assays, decreasing concentrations of SL-containing extracts in PBS (1% DMSO) were tested in replicates with 1% DMSO in PBS as negative controls. HPLC demonstrated similar concentrations of most SL in both extracts. However, Spadona-extract contained significantly higher concentrations of 11, 13-dihydro-8-deoxylactucin (P = 0.01), while Puna II-extract had increased levels of 11, 13-dihydrolactucin (P LFIA, both extracts reduced larval feeding at increasing concentrations, but Spadona-extract showed higher potency confirmed by significantly lower EC50 (P  0.05). In the AMIA, both SL-containing extracts induced a dose-dependent effect but Spadona-extract showed greater activity and exerted faster worm paralysis than Puna II-extract with significantly lower EC50 (P < 0.0001). No cuticular damage was observed by EM in worms exposed to any of the extracts. We have demonstrated that SL-containing extracts from forage chicory can inhibit feeding of free-living larvae and exert direct effects against parasitic stages of O. ostertagi. Our results may contribute to the identification of natural

  9. Gender Differences in Depressive Symptom Profile: Results from Nationwide General Population Surveys in Korea.

    Science.gov (United States)

    Kim, Ji-Hyun; Cho, Maeng Je; Hong, Jin Pyo; Bae, Jae Nam; Cho, Seong-Jin; Hahm, Bong-Jin; Lee, Dong-Woo; Park, Jong-Ik; Lee, Jun-Young; Jeon, Hong Jin; Chang, Sung Man

    2015-11-01

    This study investigated gender differences in symptom profiles of major depressive disorder (MDD) in the Korean general population. Data were pooled from the series of nationwide Korean Epidemiologic Catchment Area surveys conducted in 2001, 2006 and 2011, respectively. Of the 18,807 participants, 507 (397 women and 110 men) were diagnosed with MDD within the prior 12 months. In agreement with previous studies, women with MDD appeared to be more vulnerable to experiencing atypical depressive episodes defined as depression with two or more symptoms of fatigue, increased appetite and hypersomnia (P gender was significantly related with higher prevalence of fatigue (P = 0.008), hypersomnia (P = 0.001), noticeable psychomotor retardation (P = 0.029) and suicidal attempts (P = 0.016) with adjustment for birth cohort effect, partner status, and employment status. In the same analysis, men with MDD appeared more vulnerable to decreased libido than women (P = 0.009). This is the first report to demonstrate gender differences in symptomatology of MDD in the general Korean population, and the results are comparable to previous investigations from western societies. Assumingly, the intercultural similarity in female preponderance to atypical depression might reflect the common biological construct underlying the gender difference in mechanism of MDD. In clinical settings, gender differences of MDD should be carefully considered, because these features could be related with treatment response and drug side effects.

  10. Troublesome Tinnitus in Children: Epidemiology, Audiological Profile, and Preliminary Results of Treatment

    Directory of Open Access Journals (Sweden)

    G. Bartnik

    2012-01-01

    Results. The study showed that 41.3% of the children suffered from bothersome tinnitus. In this group 44.1% of the patients demonstrated normal hearing. The success of the therapy after 6 months was estimated on 81.4% of significant improvement. Conclusions. It is recommended that a questionnaire include an inquiry about the presence of tinnitus during hearing screening tests.

  11. Neuropsychological profile of pre-schoolers with metaphonological difficulties: results from a non-clinical sample.

    Science.gov (United States)

    Termine, C; Stella, G; Capsoni, C; Rosso, E; Binda, A; Pirola, A; Conti, C; Gruppi, E; Lanzi, G; Salini, S; Tognatti, C; Zoppello, M; Balottin, U

    2007-11-01

    The level of language development reached in pre-school age is considered the most reliable predictor of reading acquisition. In normally developing children, learning to read is strongly related to early language skills, and in particular to phonological processing abilities. In dyslexic children, reading abilities seem to show a correlation with phonological awareness. A group of 65 children (aged 5-6 years) were recruited and submitted to an in-depth neuropsychological assessment [i.e. metaphonological skills, intelligence, verbal short-term memory (VSTM) and other aspects of receptive and expressive language]. We were able to identify 14 children with significant metaphonological difficulties (MD): 11 children with exclusively MD, and the other three children with specific language impairment. This study compares the neuropsychological profile obtained from children with MD with that of a peer group without any language impairment (N). The performances of the MD were within the normal ability range in almost all the administered tests but significantly lower compared with those of their peers without language impairment (N) in some items of the intelligence scale (Wechsler Preschool and Primary Scale of Intelligence) and in the tests of VSTM and of receptive/expressive language. Nevertheless, there were not statistically significant differences between MD and N in output phonology. In pre-school age, in a group of non-clinical children, with a range of abilities, those with MD appear to be at the lower end of the normal range in many other verbal skills. These children could be considered at-risk for possible subsequent difficulties learning to read and thus need to be identified and to warrant prompt treatment.

  12. Accelerating activity coefficient calculations using multicore platforms, and profiling the energy use resulting from such calculations.

    Science.gov (United States)

    Topping, David; Alibay, Irfan; Bane, Michael

    2017-04-01

    To predict the evolving concentration, chemical composition and ability of aerosol particles to act as cloud droplets, we rely on numerical modeling. Mechanistic models attempt to account for the movement of compounds between the gaseous and condensed phases at a molecular level. This 'bottom up' approach is designed to increase our fundamental understanding. However, such models rely on predicting the properties of molecules and subsequent mixtures. For partitioning between the gaseous and condensed phases this includes: saturation vapour pressures; Henrys law coefficients; activity coefficients; diffusion coefficients and reaction rates. Current gas phase chemical mechanisms predict the existence of potentially millions of individual species. Within a dynamic ensemble model, this can often be used as justification for neglecting computationally expensive process descriptions. Indeed, on whether we can quantify the true sensitivity to uncertainties in molecular properties, even at the single aerosol particle level it has been impossible to embed fully coupled representations of process level knowledge with all possible compounds, typically relying on heavily parameterised descriptions. Relying on emerging numerical frameworks, and designed for the changing landscape of high-performance computing (HPC), in this study we focus specifically on the ability to capture activity coefficients in liquid solutions using the UNIFAC method. Activity coefficients are often neglected with the largely untested hypothesis that they are simply too computationally expensive to include in dynamic frameworks. We present results demonstrating increased computational efficiency for a range of typical scenarios, including a profiling of the energy use resulting from reliance on such computations. As the landscape of HPC changes, the latter aspect is important to consider in future applications.

  13. Patient-reported multiple drug reactions: Clinical profile and results of challenge testing

    Directory of Open Access Journals (Sweden)

    Ramam M

    2010-01-01

    Full Text Available Background: Some patients report hypersensitivity reactions to many drugs making it difficult to prescribe medications when they fall ill. Aim: To describe the clinical profile of multiple drug hypersensitivity and the results of challenge testing in a large teaching hospital.Methods: We performed a five-year retrospective review of the records of patients who complained of reactions to two or more unrelated drugs and avoided medication because of a fear of developing reactions. Oral challenge testing was carried out in hospital with drugs suspected by the patient to cause reactions and/or commonly prescribed medications. A positive reaction was diagnosed when symptoms and signs resembled previously experienced episodes and there was no such reaction with placebo. Results: Twenty three patients (aged 14-65 years; 19 females underwent challenge testing. Their complaints had been present for 1-30 years, with 2-40 drug reaction episodes reported. Antibiotics and non-steroidal anti-inflammatory drugs (NSAIDs were most commonly implicated, and urticaria/angioedema were the most often reported manifestations. The patients underwent 3-27 challenges with 1-24 drugs. Three had positive challenge reactions with various NSAIDs, 13 developed symptoms and signs that were judged not to be true reactions, and 7 had no reactions. None of our patients qualified for a diagnosis of true multiple drug hypersensitivity. Conclusion: Patients who believe they are allergic to multiple, pharmacologically unrelated drugs are usually mistaken. Challenge testing is a reliable way of demonstrating this and providing patients with a list of safe drugs.

  14. Differences in Microsatellite Instability Profiles between Endometrioid and Colorectal Cancers: A Potential Cause for False-Negative Results?

    Science.gov (United States)

    Wang, Yang; Shi, Chanjuan; Eisenberg, Rosana; Vnencak-Jones, Cindy L

    2017-01-01

    Colorectal (CRCs) and endometrioid (EMCs) cancers in patients with Lynch syndrome exhibit microsatellite instability (MSI) detected by PCR or immunohistochemistry (IHC). While both assays are equally sensitive for CRCs, some suggest that PCR has a higher false-negative rate than IHC in EMCs. We assessed the MSI profiles of 91 EMC and 311 CRC specimens using five mononucleotide repeat markers: BAT25, BAT26, NR21, NR24, and MONO27. EMCs with high MSI (MSI-H) showed a mean left shift of 3 nucleotides (nt), which was significantly different from 6 nt in CRCs. A shift of 1 nt was observed in multiple markers in 76% of MSI-H EMCs, whereas only 12% of MSI-H CRCs displayed a 1-nt shift in one of five markers. IHC against four mismatch repair proteins was performed in 78 EMCs. Loss of staining in one or more proteins was detected in 18 of 19 tumors that were MSI-H by PCR. When EMC tumor cell burden was diluted to <30%, MSI-H was no longer observed in two of three EMCs with a mean nucleotide shift of 1 nt. These results indicate that EMC and CRC MSI profiles are different and that caution should be exercised when interpreting the results, as subtle, 1-nt changes may be missed. These findings provide a potential cause of previously reported discordant MSI and IHC results in EMCs. Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology. Published by Elsevier Inc. All rights reserved.

  15. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi

    Directory of Open Access Journals (Sweden)

    Miguel Peña-Espinoza

    2015-12-01

    Full Text Available The study investigated direct anthelmintic effects of sesquiterpene lactones (SL-containing extracts from forage chicory against free-living and parasitic stages of Ostertagia ostertagi. Freeze-dried leaves from chicory cultivars ‘Spadona’ and ‘Puna II’ were extracted using methanol/water. Total SL were further fractionated by solid-phase extraction and resulting extracts were characterised by high-performance liquid chromatography (HPLC. O. ostertagi eggs from faeces of mono-infected calves were hatched and L1 were used in a larval feeding inhibition assay (LFIA, while cultured L3 were used in a larval exsheathment inhibition assay (LEIA. Adult worms were immediately recovered after slaughter and used for motility inhibition assays (AMIA. Electron microscopy (EM was performed on adult O. ostertagi exposed to 1000 μg extract mL−1 of both chicory cultivars. In all assays, decreasing concentrations of SL-containing extracts in PBS (1% DMSO were tested in replicates with 1% DMSO in PBS as negative controls. HPLC demonstrated similar concentrations of most SL in both extracts. However, Spadona-extract contained significantly higher concentrations of 11, 13-dihydro-8-deoxylactucin (P = 0.01, while Puna II-extract had increased levels of 11, 13-dihydrolactucin (P  0.05. In the AMIA, both SL-containing extracts induced a dose-dependent effect but Spadona-extract showed greater activity and exerted faster worm paralysis than Puna II-extract with significantly lower EC50 (P < 0.0001. No cuticular damage was observed by EM in worms exposed to any of the extracts. We have demonstrated that SL-containing extracts from forage chicory can inhibit feeding of free-living larvae and exert direct effects against parasitic stages of O. ostertagi. Our results may contribute to the identification of natural anti-parasitic compounds and to interpret the in vivo anthelmintic effects of forage chicory.

  16. Relationship Between Soil and Essential Oil Profiles in Salvia desoleana Populations: Preliminary Results.

    Science.gov (United States)

    Rapposelli, Emma; Melito, Sara; Barmina, Giovanni Gabriele; Foddai, Marzia; Azara, Emanuela; Scarpa, Grazia Maria

    2015-09-01

    Salvia desoleana is a herbaceous perennial shrub endemic of Sardinia (Italy). The leaves are a source of essential oil, used in pharmaceutical and cosmetic industries. The therapeutic function of this species has been associated to the presence of essential oils rich in α/β-pinene, p-cimene, linalool, linalyl acetate and 1,8-cineole. Today.the industrial request of Salvia essential oils is increasing and most of the biomass is exploited from the natural populations which are under severe risk of genetic erosion. In order to improve the essential oil production, the study of the environmental parameters that influence composition, quality and quantity of the essential oils, turns out to be necessary. Soil physical and chemical structure represents one of the determinant factors in secondary metabolites production, and could also be involved in volatiles fraction composition in the same species. The main aim of this research was to explore the relationship between essential oil profiles and soil characteristics in S. desoleana populations. GC/MS analysis performed on the essential oil extracts identified 22 principal compounds, which were extremely variable among the five S. desoleana populations studied. The analysis of the essential oils revealed different compositions in the terpenes fractions: 68.2% of monoterpenes, 27.3% of sesquiterpenes and 4.5% of diterpenes. Analysis of chemical and physical soil parameters at the collection sites revealed that silt and sand contents were correlated with α-pinene and sclareol fractions and the total K20 was significantly correlated to several compounds belonging to the three terpene fractions identified. These results will provide guidelines for the in site conservation and for the improvement of the commercial value of the species.

  17. Comorbidities with chronic physical conditions and gender profiles of illness in schizophrenia. Results from PREST, a new health dataset.

    Science.gov (United States)

    Gabilondo, Andrea; Alonso-Moran, Edurne; Nuño-Solinis, Roberto; Orueta, Juan F; Iruin, Alvaro

    2017-02-01

    Using data from a large health dataset, the objectives are to describe the epidemiology of comorbidities with chronic physical conditions in schizophrenia, to identify gender profiles of illness and to discuss findings in the light of previous research. The PREST health database was used which combines high quality and complementary data from numerous public health care resources in the Basque Country (Spain). A total number of 2,255,406 patients were included in this study and 7331 had a diagnosis of schizophrenia. 55.6% of them had one comorbid condition and 29.3% had 2 or more (e.g. multiple comorbidities). Hypertension (16.8%) was the most prevalent diagnosed comorbid condition in these patients. The risk of having neuropsychiatric disorders including Parkinson (OR up to 47.89), infectious diseases (OR up to 3.31) or diabetes (OR2.23) was increased, while the risk of having cancer (OR down to 0.76) or some cardiovascular conditions (OR down to 0.63) was reduced. Women (both with and without schizophrenia) showed higher percentages of comorbidities than men. A cluster of respiratory diseases was found only in women with schizophrenia (not in men). Results confirm partially previous findings and call for a more proactive and comprehensive approach to the health care of patients with schizophrenia. Specific profiles of risks for concrete disorders were identified which could be explained by selective underdiagnoses or higher exposition to risk factors in this group of patients. Results also suggest the need of a more gender oriented approach to health care in schizophrenia. Copyright © 2016 Elsevier Inc. All rights reserved.

  18. Guanine α-carboxy nucleoside phosphonate (G-α-CNP) shows a different inhibitory kinetic profile against the DNA polymerases of human immunodeficiency virus (HIV) and herpes viruses.

    Science.gov (United States)

    Balzarini, Jan; Menni, Michael; Das, Kalyan; van Berckelaer, Lizette; Ford, Alan; Maguire, Nuala M; Liekens, Sandra; Boehmer, Paul E; Arnold, Eddy; Götte, Matthias; Maguire, Anita R

    2017-07-15

    α-Carboxy nucleoside phosphonates (α-CNPs) are modified nucleotides that represent a novel class of nucleotide-competing reverse transcriptase (RT) inhibitors (NcRTIs). They were designed to act directly against HIV-1 RT without the need for prior activation (phosphorylation). In this respect, they differ from the nucleoside or nucleotide RTIs [N(t)RTIs] that require conversion to their triphosphate forms before being inhibitory to HIV-1 RT. The guanine derivative (G-α-CNP) has now been synthesized and investigated for the first time. The (L)-(+)-enantiomer of G-α-CNP directly and competitively inhibits HIV-1 RT by interacting with the substrate active site of the enzyme. The (D)-(-)-enantiomer proved inactive against HIV-1 RT. In contrast, the (+)- and (-)-enantiomers of G-α-CNP inhibited herpes (i.e. HSV-1, HCMV) DNA polymerases in a non- or uncompetitive manner, strongly indicating interaction of the (L)-(+)- and the (D)-(-)-G-α-CNPs at a location different from the polymerase substrate active site of the herpes enzymes. Such entirely different inhibition profile of viral polymerases is unprecedented for a single antiviral drug molecule. Moreover, within the class of α-CNPs, subtle differences in their sensitivity to mutant HIV-1 RT enzymes were observed depending on the nature of the nucleobase in the α-CNP molecules. The unique properties of the α-CNPs make this class of compounds, including G-α-CNP, direct acting inhibitors of multiple viral DNA polymerases. Copyright © 2017 Elsevier Inc. All rights reserved.

  19. Crustal structure of the Central-Eastern Greenland: results from the Topo Greenland refraction profile

    DEFF Research Database (Denmark)

    Shulgin, Alexey; Thybo, Hans

    2014-01-01

    refraction/wide-angle reflection profile. The seismic data was acquired by a team of six people during a two-month long experiment in summer of 2011 on the ice cap in the interior of central-eastern Greenland. The presence of an up to 3.4 km thick ice sheet, permanently covering most of the land mass, made...... acquisition of geophysical data logistically complicated. The profile extends 310 km inland in E-W direction from the approximate edge of the stable ice cap near the Scoresby Sund across the center of the ice cap. 350 Reftek Texan receivers recorded high-quality seismic data from 8 equidistant shots along...... the profile. Explosive charge sizes were 1 ton at the ends and ca. 500 kg along the profile, loaded with about 125 kg at 35-85 m depth in individual boreholes. Given that the data acquisition was affected by the thick ice sheet, we questioned the quality of seismic records in such experiment setup. We have...

  20. Profile grid monitor and first measurement results at the MedAustron accelerator

    CERN Document Server

    Repovz, M; Kerschbaum, A; Osmic, F; Schwarz, S; Burtin, G

    2013-01-01

    MedAustron is a synchrotron based ion beam therapy center located in Wiener Neustadt/Austria. The MedAustron accelerator design is based on CERN’s Proton-Ion Medical Machine Study (PIMMS) [1] and is currently in the accelerator installation and beam commissioning phase. One of the basic measurements for commissioning of an accelerator is also beam profile measurement. The beam at the end of the Low Energy Beam Transport (LEBT) line and in the Medium Energy Beam Transport (MEBT) line (after the fast deflector) is pulsed. Due to pulsed beam the Wire Scanner Monitor (WSX) cannot be used. To measure a beam profile at these locations a new monitor has been developed – Profile Grid Monitor (PGX). The PGX is also known as harp grid monitor and it contains 64 wires positioned vertically and 64 wires horizontally for measuring the beam profile in both transverse planes. The PGX acquires the current of all 128 wires simultaneously, converts it to voltage, digitizes the values and processes the co...

  1. Planck intermediate results: V. Pressure profiles of galaxy clusters from the Sunyaev-Zeldovich effect

    DEFF Research Database (Denmark)

    Bartlett, J.G.; Cardoso, J.-F.; Castex, G.

    2013-01-01

    Taking advantage of the all-sky coverage and broadfrequency range of the Planck satellite, we study the Sunyaev-Zeldovich (SZ) and pressure profiles of 62 nearby massive clusters detected at high significance in the 14-month nominal survey. Careful reconstruction of the SZ signal indicates that m...

  2. Indeterminate human immunodeficiency virus Western blot profiles in ethiopians with discordant screening-assay results

    NARCIS (Netherlands)

    Meles, Hailu; Wolday, Dawit; Fontanet, Arnaud; Tsegaye, Aster; Tilahun, Tesfaye; Aklilu, Mathias; Sanders, Eduard; Rinke de Wit, Tobias F.

    2002-01-01

    The Western blot (WB) assay is the most widely accepted confirmatory assay for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1). However, indeterminate WB reactivity to HIV-1 proteins may occur in individuals who do not appear to be infected with HIV. The profiles of WB

  3. Distinct differences in global gene expression profiles in non-implanted blastocysts and blastocysts resulting in live birth

    DEFF Research Database (Denmark)

    Kirkegaard, Kirstine Kjær; Fredsted, Palle Villesen; Jensen, Jacob Malte

    2015-01-01

    Results from animal models points towards the existence of a gene expression profile that is distinguishably different in viable embryos compared with non-viable embryos. Knowledge of human embryo transcripts is however limited, in particular with regard to how gene expression is related to clini......Results from animal models points towards the existence of a gene expression profile that is distinguishably different in viable embryos compared with non-viable embryos. Knowledge of human embryo transcripts is however limited, in particular with regard to how gene expression is related...... to clinical outcome. The purpose of the present study was therefore to determine the global gene expression profiles of human blastocysts. Next Generation Sequencing was used to identify genes that were differentially expressed in non-implanted embryos and embryos resulting in live birth. Three trophectoderm...

  4. Preliminary results of the Umkehr and ozonosonde ozone profile intercomparison at Marambio Base, Antarctica

    Science.gov (United States)

    Cizkova, Klara; Metelka, Ladislav; Laska, Kamil; Stanek, Martin

    2017-04-01

    Retrieved ozone profiles can be a useful tool to monitor the current state of the stratospheric ozone layer and the recovery of the Antarctic ozone hole. At the Marambio Base (64° S, 56° W), located in the Antarctic Peninsula Region, the ozone profiles are obtained by the ozonesonde observations or the Umkehr method from the Brewer spectrophotometer B199. The instrument is operated by the Czech Hydrometeorological Institute and was installed in February 2010. The ozonesonde observations have been carried out by the Finnish Meteorological Institute since late 1980s, with the highest frequency during austral spring months. In this study, the vertical ozone profiles during spring and summer 2015 were compared. The year 2015 was selected because of the late onset of the ozone hole, which offered the possibility to start the intercomparison from the beginning of the ozone hole formation. We have selected 20 days in August-December 2015, when both Umkehr and ozonesonde measurements were available. Because the ozonesonde balloon can only ascend to altitudes of about 30 km, only Umkehr layers 0-5 were taken into account. The largest mean differences between the ozonesonde and Umkehr measurements were observed in the Umkehr layer 4 with the approximate layer base height at 19.0 km. In this level, the ozonesonde observations exceeded the Umkehr retrievals in average by 10.6 %. On the other hand, in the Umkehr layer 3 with the approximate layer base height at 14.6 km, the Umkehr ozone retrievals were in average by 10.2 % higher than the ozonesonde measurements. The Umkehr and ozonesonde profiles differed largely during the ozone hole period or when the solar zenith angle for Umkehr retrievals was low. However, the ozone profile differences in the selected days varied greatly and were further analyzed and presented in the form of case studies.

  5. Profile of Nigerians with diabetes mellitus - Diabcare Nigeria study group (2008: Results of a multicenter study

    Directory of Open Access Journals (Sweden)

    Sunday Chinenye

    2012-01-01

    Full Text Available Background: Diabetes Mellitus is the commonest endocrine-metabolic disorder in Nigeria similar to the experience in other parts of the world. The aim was to assess the clinical and laboratory profile, and evaluate the quality of care of Nigerian diabetics with a view to planning improved diabetes care. Materials and Methods: In a multicenter study across seven tertiary health centers in Nigeria, the clinical and laboratory parameters of diabetic out-patients were evaluated. Clinical parameters studied include type of diabetes, anthropometry, and blood pressure (BP status, chronic complications of diabetes, and treatment types. Laboratory data assessed included fasting plasma glucose (FPG, 2-h post-prandial (2-HrPP glucose, glycated hemoglobin (HbA1c, urinalysis, serum lipids, electrolytes, urea, and creatinine. Results: A total of 531 patients, 209 (39.4% males and 322 (60.6% females enrolled. The mean age of the patients was 57.1 ± 12.3 years with the mean duration of diabetes of 8.8 ± 6.6 years. Majority (95.4% had type 2 diabetes mellitus (DM compared to type 1 DM (4.6%, with P < 0.001. The mean FPG, 2-HrPP glucose, and HbA1c were 8.1 ± 3.9 mmol/L, 10.6 ± 4.6 mmol/L, and 8.3 ± 2.2%, respectively. Only 170 (32.4% and 100 (20.4% patients achieved the ADA and IDF glycemic targets, respectively. Most patients (72.8% did not practice self-monitoring of blood glucose. Hypertension was found in 322 (60.9%, with mean systolic BP 142.0 ± 23.7 mmHg, and mean diastolic BP 80.7 ± 12.7 mmHg. Diabetic complications found were peripheral neuropathy (59.2%, retinopathy (35.5%, cataracts (25.2%, cerebrovascular disease (4.7%, diabetic foot ulcers (16.0%, and nephropathy (3.2%. Conclusion: Most Nigerian diabetics have suboptimal glycemic control, are hypertensives, and have chronic complications of DM. Improved quality of care and treatment to target is recommended to reduce diabetes-related morbidity and mortality.

  6. Sesquiterpene lactone containing extracts from two cultivars of forage chicory (Cichorium intybus) show distinctive chemical profiles and in vitro activity against Ostertagia ostertagi

    DEFF Research Database (Denmark)

    Pena-Espinoza, Miguel Angel; Boas, Ulrik; Williams, Andrew

    2015-01-01

    .01), while Puna II-extract had increased levels of 11, 13-dihydrolactucin (P EC50 (P ... interfered with the exsheathment of L3 (P > 0.05). In the AMIA, both SL-containing extracts induced a dose-dependent effect but Spadona-extract showed greater activity and exerted faster worm paralysis than Puna II-extract with significantly lower EC50 (P

  7. Activating FLT3 mutants show distinct gain-of-function phenotypes in vitro and a characteristic signaling pathway profile associated with prognosis in acute myeloid leukemia.

    Directory of Open Access Journals (Sweden)

    Hanna Janke

    Full Text Available About 30% of patients with acute myeloid leukemia (AML harbour mutations of the receptor tyrosine kinase FLT3, mostly internal tandem duplications (ITD and point mutations of the second tyrosine kinase domain (TKD. It was the aim of this study to comprehensively analyze clinical and functional properties of various FLT3 mutants. In 672 normal karyotype AML patients FLT3-ITD, but not FLT3-TKD mutations were associated with a worse relapse free and overall survival in multivariate analysis. In paired diagnosis-relapse samples FLT3-ITD showed higher stability (70% compared to FLT3-TKD (30%. In vitro, FLT3-ITD induced a strong activating phenotype in Ba/F3 cells. In contrast, FLT3-TKD mutations and other point mutations--including two novel mutations--showed a weaker but clear gain-of-function phenotype with gradual increase in proliferation and protection from apoptosis. The pro-proliferative capacity of the investigated FLT3 mutants was associated with cell surface expression and tyrosine 591 phosphorylation of the FLT3 receptor. Western blot experiments revealed STAT5 activation only in FLT3-ITD positive cell lines, in contrast to FLT3-non-ITD mutants, which displayed an enhanced signal of AKT and MAPK activation. Gene expression analysis revealed distinct difference between FLT3-ITD and FLT3-TKD for STAT5 target gene expression as well as deregulation of SOCS2, ENPP2, PRUNE2 and ART3. FLT3-ITD and FLT3 point mutations show a gain-of-function phenotype with distinct signalling properties in vitro. Although poor prognosis in AML is only associated with FLT3-ITD, all activating FLT3 mutations can contribute to leukemogenesis and are thus potential targets for therapeutic interventions.

  8. Further results on binary convolutional codes with an optimum distance profile

    DEFF Research Database (Denmark)

    Johannesson, Rolf; Paaske, Erik

    1978-01-01

    Fixed binary convolutional codes are considered which are simultaneously optimal or near-optimal according to three criteria: namely, distance profiled, free distanced_{ infty}, and minimum number of weightd_{infty}paths. It is shown how the optimum distance profile criterion can be used to limit...... the search for codes with a large value ofd_{infty}. We present extensive lists of such robustly optimal codes containing rateR = l/2nonsystematic codes, several withd_{infty}superior to that of any previously known code of the same rate and memory; rateR = 2/3systematic codes; and rateR = 2/3nonsystematic...... codes. As a counterpart to quick-look-in (QLI) codes which are not "transparent," we introduce rateR = 1/2easy-look-in-transparent (ELIT) codes with a feedforward inverse(1 + D,D). In general, ELIT codes haved_{infty}superior to that of QLI codes....

  9. The gene expression profiling of hepatocellular carcinoma by a network analysis approach shows a dominance of intrinsically disordered proteins (IDPs) between hub nodes.

    Science.gov (United States)

    Singh, Sakshi; Colonna, Giovanni; Di Bernardo, Giovanni; Bergantino, Francesca; Cammarota, Marcella; Castello, Giuseppe; Costantini, Susan

    2015-11-01

    We have analyzed the transcriptomic data from patients with hepatocellular carcinoma (HCC) after viral HCV infection at the various stages of the disease by means of a networking analysis using the publicly available E-MTAB-950 dataset. The data was compared with those obtained in our group from HepG2 cells, a cancer cell line that lacks the viral infection. By sequential pruning of data, and also taking into account the data from cells of healthy patients as blanks, we were able to obtain a distribution of hub genes for the various stages that characterize the disease and finally, we isolated a metabolic sub-net specific to HCC alone. The general picture is that the basic organization to energetically and metabolically sustain the cells in both the normal and diseased conditions is the same, but a complex cluster of sub-networks controlled by hub genes drives the HCC progression with high metabolic flexibility and plasticity. In particular, we have extracted a sub-net of genes strictly correlated to other hub genes of the network from HepG2 cells, but specific for the HCC and mainly devoted to: (i) control at chromatin levels of cell division; (ii) control of ergastoplasmatic stress through protein degradation and misfolding; (iii) control of the immune response also through an increase of mature T-cells in the thymus. This sub-net is characterized by 26 hub genes coding for intrinsically disordered proteins with a high ability to interact with numerous molecular partners. Moreover, we have also noted that periphery molecules, that is, with one or very few interactions (e.g., cytokines or post-translational enzymes), which do not have a central role in the clusters that make up the global metabolic network, essentially have roles as information transporters. The results evidence a strong presence of intrinsically disordered proteins with key roles as hubs in the sub-networks that characterize the various stages of the disease, conferring a structural plasticity to

  10. Bacterial profiling of Saharan dust deposition in the Atlantic Ocean using sediment trap moorings – year one results

    Science.gov (United States)

    Munday, Chris; Brummer, Geert-Jan; van der Does, Michelle; Korte, Laura; Stuut, Jan-Berend

    2015-04-01

    Large quantities of dust are transported from the Sahara Desert across the Atlantic Ocean towards the Caribbean each year, with a large portion of it deposited in the ocean. This dust brings an array of minerals, nutrients and organic matter, both living and dead. This input potentially fertilizes phytoplankton growth, with resulting knock-on effects throughout the food chain. The input of terrestrial microbial life may also have an impact on the marine microbial community. The current multi-year project consists of a transect of floating dust collectors and sub-surface sediment traps placed at 12°N across the Atlantic Ocean. Sediment traps are located 1200m and 3500m below the sea surface and all are synchronized to collect samples for a period of two weeks. The aim is to understand the links between dust input and the bacterial community and how this relates to ocean productivity and the carbon cycle. The first set of sediment trap samples were recovered using the RV Pelagia in November 2013 with promising results. Results from 7 sediment traps (three at 1200m and four at 3500m) were obtained. In general, the total mass flux decreased as distance from the source increased and the upper traps generally held more material than those at 3500m. Denaturing Gradient Gel Electrophoresis (DGGE) was used as a screening technique, revealing highly varied profiles, with the upper (1200m) traps generally showing more variation throughout the year. Several samples have been submitted for high throughput DNA sequencing which will identify the variations in these samples.

  11. Association Between Improvement in Cardiovascular Risk Profile and Changes in Sickness Absence: Results of the ICARIA Study.

    Science.gov (United States)

    Calvo-Bonacho, Eva; Catalina-Romero, Carlos; Cabrera, Martha; Fernández-Labandera, Carlos; Sánchez Chaparro, Miguel Ángel; Brotons, Carlos; Ruilope, Luis Miguel

    2017-11-01

    The purpose of this study was to investigate whether changes in cardiovascular risk (CVR) are associated with the length and cost of sickness absence. A prospective cohort of 179 186 participants was evaluated. Each participant's CVR (SCORE) was assessed on 2 consecutive medical examinations, approximately 1 year apart (365 ± 90 days). Cardiovascular risk was categorized as < 4% or ≥ 4%, and participants were divided into 4 groups according to changes in their risk between the 2 assessments. After the second CVR estimate, a 1-year follow-up was carried out to assess sickness absence. Differences between the 4 groups in terms of the total count of sickness absence days during the follow-up period were tested using Poisson regression models. After adjustment for covariates, participants who showed an improvement in CVR had a lower count of sickness absence days compared with both those who showed a worsening in risk and those who remained stable at ≥ 4% (RR, 0.91; 95%CI, 0.84-0.98). In comparison with participants whose CVR did not improve, more of the participants whose risk did improve had quit smoking (+17.2%; P < .001), and had controlled their blood pressure (+26.0%, P < .001), total cholesterol (+9.3%; P < .001), low-density lipoprotein cholesterol (+14.9%; P < .001), and triglyceride levels (+14.6%; P < .001). Our results suggest that an improvement in CVR profile is accompanied by a decrease in sickness absence during a 1-year follow-up. Copyright © 2017 Sociedad Española de Cardiología. Published by Elsevier España, S.L.U. All rights reserved.

  12. Genomic risk profiling of ischemic stroke: results of an international genome-wide association meta-analysis.

    Directory of Open Access Journals (Sweden)

    James F Meschia

    Full Text Available Familial aggregation of ischemic stroke derives from shared genetic and environmental factors. We present a meta-analysis of genome-wide association scans (GWAS from 3 cohorts to identify the contribution of common variants to ischemic stroke risk.This study involved 1464 ischemic stroke cases and 1932 controls. Cases were genotyped using the Illumina 610 or 660 genotyping arrays; controls, with Illumina HumanHap 550Kv1 or 550Kv3 genotyping arrays. Imputation was performed with the 1000 Genomes European ancestry haplotypes (August 2010 release as a reference. A total of 5,156,597 single-nucleotide polymorphisms (SNPs were incorporated into the fixed effects meta-analysis. All SNPs associated with ischemic stroke (P<1×10(-5 were incorporated into a multivariate risk profile model.No SNP reached genome-wide significance for ischemic stroke (P<5×10(-8. Secondary analysis identified a significant cumulative effect for age at onset of stroke (first versus fifth quintile of cumulative profiles based on SNPs associated with late onset, ß = 14.77 [10.85,18.68], P = 5.5×10(-12, as well as a strong effect showing increased risk across samples with a high propensity for stroke among samples with enriched counts of suggestive risk alleles (P<5×10(-6. Risk profile scores based only on genomic information offered little incremental prediction.There is little evidence of a common genetic variant contributing to moderate risk of ischemic stroke. Quintiles based on genetic loading of alleles associated with a younger age at onset of ischemic stroke revealed a significant difference in age at onset between those in the upper and lower quintiles. Using common variants from GWAS and imputation, genomic profiling remains inferior to family history of stroke for defining risk. Inclusion of genomic (rare variant information may be required to improve clinical risk profiling.

  13. Clinical Profile and Prognosis of Patients with Right Ventricular Dilated Cardiomyopathy: Results of a Prospective Study

    Directory of Open Access Journals (Sweden)

    Ya.R. Akhmatov

    2015-12-01

    Full Text Available The aim of our study was to investigate the clinical prevalence of dilated cardiomyopathy (DCM with predominantly failure of the right-side heart (right ventricular DCM, RV-DCM, and features of the clinical course and prognosis of the disease compared to DCM with biventricular heart failure (BV-HF. The study design suggests a prospective observation of 300 patients with idiopathic DCM between 2000 and 2012. Herewith, we followed the criteria of the WHO/ISFC Task Force (1995 on the Definationa and Classification of Cardiomyopathies. All patients underwent a comprehensive examination. Two groups were formed for further comparative analysis. Group 1 included 22 patients (mean age 42.9±14.3 years, male/female 5/17 with RV-DCM. Group 2 included 38 patients (mean age 43.6±13.8, male/female 29/9 with DCM and BV-HF. The groups were matched for age, sex, NYHA class II-III, and disease duration. According to our aim, we studied 5-year survival prognosis and analyzed the incidence and causes of deaths, as well as the occurrence of nonfatal complications of the disease. Medical therapy for DCM patients was performed according to the CHF therapy guidelines (ACC/AHA 2001, 2005. The results of our investigations during many years of research have shown that the clinical incidence of RV-DCM was 7.3% among all forms of DCM. The study of life prognosis in patients with 2 forms of DCM showed that 5-year mortality of patients was about 50%. Herewith, we detected the differences in causes of death depending on the type of heart damage, primarily development of fatal pulmonary embolism.

  14. High-Resolution Seismic Reflection Profiling Across the Black Hills Fault, Clark County, Nevada: Preliminary Results

    Science.gov (United States)

    Zaragoza, S. A.; Snelson, C. M.; Jernsletten, J. A.; Saldana, S. C.; Hirsch, A.; McEwan, D.

    2005-12-01

    The Black Hills fault (BHF) is located in the central Basin and Range Province of western North America, a region that has undergone significant Cenozoic extension. The BHF is an east-dipping normal fault that forms the northwestern structural boundary of the Eldorado basin and lies ~20 km southeast of Las Vegas, Nevada. A recent trench study indicated that the fault offsets Holocene strata, and is capable of producing Mw 6.4-6.8 earthquakes. These estimates indicate a subsurface rupture length at least 10 km greater than the length of the scarp. This poses a significant hazard to structures such as the nearby Hoover Dam Bypass Bridge, which is being built to withstand a Mw 6.2-7.0 earthquake on local faults. If the BHF does continue in the subsurface, this structure, as well as nearby communities (Las Vegas, Boulder City, and Henderson), may not be as safe as previously expected. Previous attempts to image the fault with shallow seismics (hammer source) were inconclusive. However, gravity studies imply that the fault continues south of the scarp. Therefore, a new experiment utilizing high-resolution seismic reflection was performed to image subsurface geologic structures south of the scarp. At each shot point, a stack of four 30-160 Hz vibroseis sweeps of 15 s duration was recorded on a 60-channel system with 40 Hz geophones. This produced two 300 m reflection profiles, with a maximum depth of 500-600 m. A preliminary look at these data indicates the existence of two faults, potentially confirming that the BHF continues in the subsurface south of the scarp.

  15. Antisense-mediated suppression of C-hordein biosynthesis in the barley grain results in correlated changes in the transcriptome, protein profile, and amino acid composition

    DEFF Research Database (Denmark)

    Hansen, Mette; Lange, Marianne; Friis, Carsten

    2007-01-01

    Antisense- or RNAi-mediated suppression of the biosynthesis of nutritionally inferior storage proteins is a promising strategy for improving the amino acid profile of seeds. However, the potential pleiotropic effects of this on interconnected pathways and the agronomic quality traits need...... to be addressed. In the current study, a transcriptomic analysis of an antisense C-hordein line of barley was performed, using a grain-specific cDNA array. The C-hordein antisense line is characterized by marked changes in storage protein and amino acid profiles, while the seed weight is within the normal range...... and no external morphological irregularities were observed. The results of the transcriptome analysis showed excellent correlation with data on changes in the relative proportions of storage proteins and amino acid composition. The antisense line had a lower C-hordein level and down-regulated transcript encoding...

  16. Global proteomics profiling improves drug sensitivity prediction: results from a multi-omics, pan-cancer modeling approach.

    Science.gov (United States)

    Ali, Mehreen; Khan, Suleiman A; Wennerberg, Krister; Aittokallio, Tero

    2017-11-27

    Proteomics profiling is increasingly being used for molecular stratification of cancer patients and cell-line panels. However, systematic assessment of the predictive power of large-scale proteomic technologies across various drug classes and cancer types is currently lacking. To that end, we carried out the first pan-cancer, multi-omics comparative analysis of the relative performance of two proteomic technologies, targeted reverse phase protein array (RPPA) and global mass spectrometry (MS), in terms of their accuracy for predicting the sensitivity of cancer cells to both cytotoxic chemotherapeutics and molecularly-targeted anticancer compounds. Our results in two cell-line panels demonstrate how MS profiling improves drug response predictions beyond that of the RPPA or the other omics profiles when used alone. However, frequent missing MS data values complicate its use in predictive modeling and required additional filtering, such as focusing on completely-measured or known oncoproteins, to obtain maximal predictive performance. Rather strikingly, the two proteomics profiles provided complementary predictive signal both for the cytotoxic and targeted compounds. Further, information about the cellular-abundance of primary target proteins was found critical for predicting the response of targeted compounds, although the non-target features also contributed significantly to the predictive power. The clinical relevance of the selected protein markers was confirmed in cancer patient data. These results provide novel insights into the relative performance and optimal use of the widely-applied proteomic technologies, MS and RPPA, which should prove useful in translational applications, such as defining the best combination of omics technologies and marker panels for understanding and predicting drug sensitivities in cancer patients. Processed datasets, R as well as Matlab implementations of the methods are available at https://github.com/mehr-een/bemkl-rbps. mehreen

  17. Storytelling Slide Shows to Improve Diabetes and High Blood Pressure Knowledge and Self-Efficacy: Three-Year Results among Community Dwelling Older African Americans

    Science.gov (United States)

    Bertera, Elizabeth M.

    2014-01-01

    This study combined the African American tradition of oral storytelling with the Hispanic medium of "Fotonovelas." A staggered pretest posttest control group design was used to evaluate four Storytelling Slide Shows on health that featured community members. A total of 212 participants were recruited for the intervention and 217 for the…

  18. Seismic refraction profile, Kingdom of Saudi Arabia: field operations, instrumentation, and initial results

    Science.gov (United States)

    Blank, H. Richard; Healy, J.H.; Roller, John; Lamson, Ralph; Fisher, Fred; McClearn, Robert; Allen, Steve

    1979-01-01

    In February 1978 a seismic deep-refraction profile was recorded by the USGS along a 1000-km line across the Arabian Shield in western Saudi Arabia. The line begins in Paleozoic and Mesozoic cover rocks near Riyadh on the Arabian Platform, leads southwesterly across three major Precambrian tectonic provinces, traverses Cenozoic rocks of the coastal plain near Jizan (Tihamat Asir), and terminates at the outer edge of the Farasan Bank in the southern Red Sea. More than 500 surveyed recording sites were occupied, including 19 in the Farasan Islands. Six shot points were used--five on land, with charges placed mostly below water table in drill holes, and one at sea, with charges placed on the sea floor and fired from a ship. The total charge consumed was slightly in excess of 61 metric tons in 21 discrete firings. Seismic energy was recorded by means of a set of 100 newly developed portable seismic stations. Each station consists of a standard 2-Hz vertical geophone coupled to a self-contained analog recording instrument equipped with a magnetic-tape cassette. The stations were deployed in groups of 20 by five observer teams, each generally consisting of two scientist-technicians and a surveyor-guide. On the day prior to deployment, the instruments were calibrated and programmed for automatic operation by means of a specially designed device called a hand-held tester. At each of ten pre-selected recording time windows on a designated firing day, the instruments were programmed to turn on, stabilize, record internal calibration signals, record the seismic signals at three levels of amplification, and then deactivate. After the final window in the firing sequence, all instruments were retrieved and their data tapes removed for processing. A specially designed, field tape- dubbing system was utilized at shot point camps to organize and edit data recorded on the cassette tapes. The main functions of this system are to concatenate all data from each shot on any given day

  19. Experimental test results study future of physical profile of the aviation.

    Directory of Open Access Journals (Sweden)

    Orlenko N. A.

    2012-07-01

    Full Text Available He results of studies of the effectiveness of the proposed work program for the training of professional-applied physical training future pilots. Based on the analysis of the data determined by the level of physical fitness, and also revealed the dynamics of the motor characteristics of future pilots. The got results were compared in the control group of students of 1-2 courses of speciality "Aeronavigation" (40 men and to the experimental group of 1-2 of speciality "Exploitation of aircrafts" (40 men. The norms of certifying were analysed for competition physical preparation during all period of education. Conducted experiment gave an opportunity to define the level of general physical preparedness of future pilots. It is proved that in the future pilots of physical fitness are a wide range of knowledge in physical education and professional-applied physical training, which makes the learning process more effective.

  20. Transgene silencing of the Hutchinson-Gilford progeria syndrome mutation results in a reversible bone phenotype, whereas resveratrol treatment does not show overall beneficial effects

    DEFF Research Database (Denmark)

    Strandgren, Charlotte; Nasser, Hasina Abdul; McKenna, Tomás

    2015-01-01

    Hutchinson-Gilford progeria syndrome (HGPS) is a rare premature aging disorder that is most commonly caused by a de novo point mutation in exon 11 of the LMNA gene, c.1824C>T, which results in an increased production of a truncated form of lamin A known as progerin. In this study, we used a mouse...... progerin splicing give hope to patients who are affected by HGPS.-Strandgren, C., Nasser, H. A., McKenna, T., Koskela, A., Tuukkanen, J., Ohlsson, C., Rozell, B., Eriksson, M. Transgene silencing of the Hutchinson-Gilford progeria syndrome mutation results in a reversible bone phenotype, whereas...

  1. Preliminary Results for Biochemical Profile Before Slaughtering and Meat Quality of Three Beef Commercial Hybrids

    Directory of Open Access Journals (Sweden)

    ALINA NARCISA POSTOLACHE

    2014-10-01

    Full Text Available This research is subscribed of an extensive project that has as main objective the production of crossbred specialized beef hybrids from reformed Romanian Black Spotted cattle's with bulls from breeds specialized for meat production (Blue Blanch Belgique, Aberdeen-Angus and Limousin. Averaged biochemical indicators analyzed showed a normal state of animal's health. The obtained half-breeds products (bulls were raised under semi-intensively conditions and slaughtered at 12 months of age. Live weight, back-fat thickness and carcass traits were significantly influenced by breed, all fat parameters being lower at this age than at standard minimum age of slaughter for meat production (18 months. Drip loss and chemical composition were similar for the breeds. The meat was lighter and pH24 values of longissimus muscle were between 5.57 and 5.64. Sensory panel tenderness and Warner-Bratzler shear force values indicated tougher meat at RBSxAA than at RBSxL1 or RBSxBBB. Differences in meat quality were probably due to the combined effects of brute chemical composition and pH dynamic during ripening. It is concluded that slaughtering steers at younger ages may require supplementary feeding, being recommended a slaughter age between 18 and 25 months old.

  2. Evaluation of clinical and inflammatory profile in opioid addiction patients with comorbid pain: results from a multicenter investigation

    Directory of Open Access Journals (Sweden)

    Dennis BB

    2014-11-01

    chemokine (C–C motif ligand 2 [CCL2]. The study objectives were addressed using a descriptive statistical summary and a multivariable logistic regression model constructed in STATA version 12.Results: Among the participants eligible for inclusion (n=235, serum IFN-γ level and substance abuse behavior proved to be important delineating characteristics for the detection of comorbid pain. Analysis of inflammatory profile showed IFN-γ to be significantly elevated among patients reporting comorbid pain (odds ratio [OR]: 2.02; 95% confidence interval [CI]: 1.17, 3.50; P=0.01. Patients reporting comorbid pain were also found to have an increase in positive opioid urine screens (OR: 1.02; 95% CI: 1.00, 1.03; P=0.01, indicating an increase in illicit opioid consumption.Conclusion: MMT patients with comorbid pain were shown to have elevated IFN-γ and higher rates of continued opioid abuse. The ability to objectively distinguish between patients with comorbid pain may help to both improve the prediction of poor responders to MMT as well as identify treatment approaches such as anti-inflammatory medications as safe alternatives for MMT patients with comorbid pain. Keywords: methadone maintenance treatment, inflammatory markers, TNF-α, IFN-γ, interleukins, CCL2, Brief Pain Inventory, opioid dependence

  3. Antigen-specific CD4 T cells are induced after intravesical BCG-instillation therapy in patients with bladder cancer and show similar cytokine profiles as in active tuberculosis.

    Directory of Open Access Journals (Sweden)

    Julia Elsäßer

    Full Text Available Specific T cell immunity in patients with active tuberculosis is associated with a decrease in multifunctionality. However, it is unknown whether cytokine profiles differ in patients with primary infection and those with prior contact. We therefore used intravesical immunotherapy with attenuated live Bacille Calmette-Guérin (BCG in patients with urothelial carcinoma as a model to characterise the induction of systemic immunity towards purified protein derivate (PPD and to study whether cytokine profiles differ depending on pre-existing immunity. Eighteen patients with non-muscle invasive bladder cancer were recruited during the BCG-induction course. Fifty-four healthy individuals served as controls. Interferon (IFN-γ and interleukin (IL-2 producing PPD-specific CD4 T cells were analysed longitudinally before each instillation using a rapid flow-cytometric whole blood immunoassay. Baseline levels of IFN-γ producing PPD-specific T cells were comparable to controls. T cells showed a 5-fold increase to 0.23% by week 2/3, and further increased 8-fold by week 4/5 (to 0.42%, p=0.0007. Systemic immunity was induced in all patients, although the increase was less pronounced in patients with pre-existing immunity. As in active TB, cytokine profiling during therapy revealed a lower percentage of multifunctional IFN-γ/IL-2 double-positive T cells compared to controls (60.2% vs. 71.9%, p=0.0003. Of note, when comparing patients with and without pre-existing immunity, cytokine profiles in patients with primary immunity were shifted towards IL-2 single producing T cells (p=0.02, whereas those in patients with pre-existing immunity were shifted towards IFN-γ single-positivity (p=0.01. In conclusion, systemic T cell responses were induced after BCG-therapy, and their kinetics and cytokine profile depended on pre-existing immunity. Decreased functionality is a typical feature of specific immunity in both patients with active tuberculosis and BCG

  4. Showing results? An analysis of the perceptions of internal and external stakeholders of the public performance communication by the Belgian and Dutch Railways

    NARCIS (Netherlands)

    Gelders, Dave; Galetzka, Mirjam; Verckens, Jan Pieter; Seydel, E.R.

    2008-01-01

    Information Quarterly, 15: 153–156). In Belgium and the Netherlands, one Performance measurement and communicating about it with the broader public is not self-evident if one looks at public services organizations (Hernon, 1998 P. Hernon, The government performance and results act. Government

  5. Cells exposed to a huntingtin fragment containing an expanded polyglutamine tract show no sign of ion channel formation: results arguing against the ion channel hypothesis

    DEFF Research Database (Denmark)

    Nørremølle, Anne; Grunnet, Morten; Hasholt, Lis

    2003-01-01

    Ion channels formed by expanded polyglutamine tracts have been proposed to play an important role in the pathological processes leading to neurodegeneration in Huntington's disease and other CAG repeat diseases. We tested the capacity of a huntingtin fragment containing an expanded polyglutamine ...... in the currents recorded in any of the two expression systems, indicating no changes in ion channel activity. The results therefore argue against the proposed hypothesis of expanded polyglutamines forming ion channels....

  6. Clinical and Epidemiological Profile of Breast Cancer in Mexico: Results of the Seguro Popular.

    Science.gov (United States)

    Reynoso-Noverón, Nancy; Villarreal-Garza, Cynthia; Soto-Perez-de-Celis, Enrique; Arce-Salinas, Claudia; Matus-Santos, Juan; Ramírez-Ugalde, María Teresa; Alvarado-Miranda, Alberto; Cabrera-Galeana, Paula; Meneses-García, Abelardo; Lara-Medina, Fernando; Bargalló-Rocha, Enrique; Mohar, Alejandro

    2017-12-01

    Purpose One half of the Mexican population lacks comprehensive health care coverage. In 2003, a reform to the General Health Law was approved that led to the creation of the System of Social Protection in Health and made universal health coverage mandatory. The main innovation of this reform was Seguro Popular, which provided coverage for breast cancer. Here we report the outcomes of women with breast cancer treated at a cancer center in Mexico under Seguro Popular. Materials and Methods This was a retrospective cohort study that included all patients with breast cancer treated in the Instituto Nacional de Cancerología in Mexico City between January 2007 and December 2013 with Seguro Popular coverage. Demographic and clinical information were collected and survival outcomes were analyzed. Results A total of 4,300 women with breast cancer were included in this analysis. Most patients had locally advanced disease at diagnosis (53%, n = 2,293), and 13% (n = 558) presented with stage IV disease. Neoadjuvant chemotherapy was administered to 1,834 patients (52%), with a pathologic complete response in 25.1% (n = 460). Median follow-up was 40.5 months. Five-year survival for the entire cohort was 82% (95% CI, 81% to 84%). Five-year survival was 97% for early-stage disease (95% CI, 95% to 98%), 82% for locally advanced disease (95% CI, 80% to 84%), and 36% for metastatic disease (95% CI, 30% to 42%). Conclusion This represents the first description of a cohort of patients with breast cancer treated in Mexico under Seguro Popular. Seguro Popular has allowed our institution, and other Mexican centers, to establish efficient standardized mechanisms to treat patients with breast cancer.

  7. Empty polyetheretherketone (PEEK) cages in anterior cervical diskectomy and fusion (ACDF) show slow radiographic fusion that reduces clinical improvement: results from the prospective multicenter "PIERCE-PEEK" study.

    Science.gov (United States)

    Suess, Olaf; Schomaker, Martin; Cabraja, Mario; Danne, Marco; Kombos, Theodoros; Hanna, Michael

    2017-01-01

    Anterior cervical diskectomy and fusion (ACDF) is a well-established surgical treatment for radiculopathy and myelopathy. Previous studies showed that empty PEEK cages have lower radiographic fusion rates, but the clinical relevance remains unclear. This paper's aim is to provide high-quality evidence on the outcomes of ACDF with empty PEEK cages and on the relevance of radiographic fusion for clinical outcomes. This large prospective multicenter clinical trial performed single-level ACDF with empty PEEK cages on patients with cervical radiculopathy or myelopathy. The main clinical outcomes were VAS (0-10) for pain and NDI (0-100) for functioning. Radiographic fusion was evaluated by two investigators for three different aspects. The median (range) improvement of the VAS pain score was: 3 (1-6) at 6 months, 3 (2-8) at 12 months, and 4 (2-8) at 18 months. The median (range) improvement of the NDI score was: 12 (2-34) at 6 months, 18 (4-46) at 12 months, and 22 (2-44) at 18 months. Complete radiographic fusion was reached by 126 patients (43%) at 6 months, 214 patients (73%) at 12 months, and 241 patients (83%) at 18 months. Radiographic fusion was a highly significant (p PEEK cages is slow and insufficient. Lack of complete radiographic fusion leads to less improvement of pain and disability. We recommend against using empty uncoated pure PEEK cages in ACDF. ISRCTN42774128. Retrospectively registered 14 April 2009.

  8. [First results of a German second opinion program show high patient satisfaction and large discrepancies between initial therapy recommendations and second opinion].

    Science.gov (United States)

    Weyerstraß, Jan; Prediger, Barbara; Neugebauer, Edmund; Pieper, Dawid

    2018-02-23

    Although legally anchored, there are no empirical results from German second opinion programs. In this study, various aspects within a population of a second opinion program are examined. In this study patients were analyzed who sought a second opinion in the period from August 2011 to December 2016. Differences in patient characteristics, differentiated by agreement of first and second opinion, were analyzed using multivariate logistic regression. Patients' satisfaction and quality of life were examined one, three and six months after obtaining the second opinion. In total, 1,414 patients sought a second opinion. Most frequent medical indications were the knee (38.7 %), the back (26.8 %), the hip (11.7 %), and the shoulder (10.2 %). Except for the indication (p=0.035), no patient characteristic had influence on the conformation of the second opinion. Approximately two out of three initial recommendations were not confirmed by the specialists. 89 % of the patients were satisfied or very satisfied with the second opinion and the service offered. The second opinion offers patients the opportunity to seek an additional independent medical opinion and thus provide support for decision making. Further research is needed to examine the reasons for the high discrepancies between the first and second opinions. Copyright © 2018. Published by Elsevier GmbH.

  9. Results of the Updated NASA Kennedy Space Center 50-MHz Doppler Radar Wind Profiler Operational Acceptance Test

    Science.gov (United States)

    Barbre', Robert E., Jr.; Deker, Ryan K.; Leahy, Frank B.; Huddleston, Lisa

    2016-01-01

    We present here the methodology and results of the Operational Acceptance Test (OAT) performed on the new Kennedy Space Center (KSC) 50-MHz Doppler Radar Wind Profiler (DRWP). On day-of-launch (DOL), space launch vehicle operators have used data from the DRWP to invalidate winds in prelaunch loads and trajectory assessments due to the DRWP's capability to quickly identify changes in the wind profile within a rapidly-changing wind environment. The previous DRWP has been replaced with a completely new system, which needs to undergo certification testing before being accepted for use in range operations. The new DRWP replaces the previous three-beam system made of coaxial cables and a copper wire ground plane with a four-beam system that uses Yagi antennae with enhanced beam steering capability. In addition, the new system contains updated user interface software while maintaining the same general capability as the previous system. The new DRWP continues to use the Median Filter First Guess (MFFG) algorithm to generate a wind profile from Doppler spectra at each range gate. DeTect (2015) contains further details on the upgrade. The OAT is a short-term test designed so that end users can utilize the new DRWP in a similar manner to the previous DRWP during mission operations at the Eastern Range in the midst of a long-term certification process. This paper describes the Marshall Space Flight Center Natural Environments Branch's (MSFC NE's) analyses to verify the quality and accuracy of the DRWP's meteorological data output as compared to the previous DRWP. Ultimately, each launch vehicle program has the responsibility to certify the system for their own use.

  10. ADVERSE EVENT OF DRUG THERAPY (THE FIRST RESULTS OF THE STUDY ACCORDING TO THE PROFILE OUTPATIENT REGISTER

    Directory of Open Access Journals (Sweden)

    Yu. V. Lukina

    2016-01-01

    Full Text Available Adverse events (As of medicines are becoming a more serious problem of health care and society due to the growth of the pharmaceutical market. Incomplete information about AEs of drug therapy is an important aspect of this problem. The use of the register method is very promising in the study of adverse drug reactions in clinical practice. Aim. To study the AEs of drug therapy in the PROFILE outpatient register. Material and methods. Primary patients (n=1531 were included into the PROFILE outpatient register from January 2011 to August 2015. The median age of patients was 63 (54; 71 years. The registration card of patients and patient questionnaires were used within the register for the database creation. Analysis of retrospective data of the register was fulfilled Results. Various AEs were reported in 223 (14.6% patients. Total anamnestic information on 301 cases of AEs was collected: 223 cases were recorded once, 63 – twice, 15 – three times. Patients with AEs were older than patients without AEs ((p<0.0001. AEs were registered most frequently in the use of ACE inhibitors and acetylsalicylic acid: an average of 15% of all cases, each. Various allergic reactions and symptoms of gastrointestinal disorders (pain, nausea, vomiting, diarrhea, etc. were leaders in the structure of AEs. Naturally there were more patients without AEs among those, who did not take medications. Among patients treated with 6 to 10 medications per day, there were 1.5 times more people who had at least one AE (p<0.0001. Conclusion. The use of the register method allows to add information about the safety of various drugs. However, analysis of the prospective data of the PROFILE register looks the most promising for the solution of this problem.

  11. Atmospheric boundary layer wind profile at a flat coastal site – wind speed lidar measurements and mesoscale modeling results

    DEFF Research Database (Denmark)

    Floors, Rogier; Batchvarova, Ekaterina; Gryning, Sven-Erik

    2011-01-01

    Wind profiles up to 600m height are investigated. Measurements of mean wind speed profiles were obtained from a novel wind lidar and compared to model simulations from a mesoscale model (WRFARW v3.1). It is found that WRF is able to predict the mean wind profile rather well and typically within 1......–2ms−1 to the individual measured values. WRF underpredicts the normalized wind profile, especially for stable conditions. The effect of baroclinicity on the upper part of the wind profile is discussed....

  12. The disruption of Celf6, a gene identified by translational profiling of serotonergic neurons, results in autism-related behaviors.

    Science.gov (United States)

    Dougherty, Joseph D; Maloney, Susan E; Wozniak, David F; Rieger, Michael A; Sonnenblick, Lisa; Coppola, Giovanni; Mahieu, Nathaniel G; Zhang, Juliet; Cai, Jinlu; Patti, Gary J; Abrahams, Brett S; Geschwind, Daniel H; Heintz, Nathaniel

    2013-02-13

    The immense molecular diversity of neurons challenges our ability to understand the genetic and cellular etiology of neuropsychiatric disorders. Leveraging knowledge from neurobiology may help parse the genetic complexity: identifying genes important for a circuit that mediates a particular symptom of a disease may help identify polymorphisms that contribute to risk for the disease as a whole. The serotonergic system has long been suspected in disorders that have symptoms of repetitive behaviors and resistance to change, including autism. We generated a bacTRAP mouse line to permit translational profiling of serotonergic neurons. From this, we identified several thousand serotonergic-cell expressed transcripts, of which 174 were highly enriched, including all known markers of these cells. Analysis of common variants near the corresponding genes in the AGRE collection implicated the RNA binding protein CELF6 in autism risk. Screening for rare variants in CELF6 identified an inherited premature stop codon in one of the probands. Subsequent disruption of Celf6 in mice resulted in animals exhibiting resistance to change and decreased ultrasonic vocalization as well as abnormal levels of serotonin in the brain. This work provides a reproducible and accurate method to profile serotonergic neurons under a variety of conditions and suggests a novel paradigm for gaining information on the etiology of psychiatric disorders.

  13. Young people's awareness on biobanking and DNA profiling: results of a questionnaire administered to Italian university students.

    Science.gov (United States)

    Tozzo, Pamela; Fassina, Antonio; Caenazzo, Luciana

    2017-12-01

    Current policy approaches to social and ethical issues surrounding biobanks manifest lack of public information given by researchers and government, despite the evidence that Italian citizens are well informed about technical and other public perspectives of biotechnologies. For this reason, the focus of our survey was to interview our University's students on these aspects. The sample consisted of Padua University students (N = 959), who were administered a questionnaire comprising eight questions covering their knowledge about biobanks, their perception of the related benefits and risks, their willingness to donate samples to a biobank for research purposes, their attitude to having their own DNA profile included in a forensic DNA database, and the reasons behind their answers. The vast majority of the students invited to take part in the survey completed the questionnaire, and the number of participants sufficed to be considered representative of the target population. Despite the respondents' unfamiliarity with the topics explored, suggested by the huge group of respondents answering "I don't know" to the questions regarding Itaian regulation and reality, their answers demonstrate a general agreement to participate in a biobanking scheme for research purposes, as expressed by the 91% of respondents who were reportedly willing to donate their samples. As for the idea of a forensic DNA database, 35% of respondents said they would agree to having their profile included in such a database, even if they were not fully aware of the benefits and risks of such action.This study shows that Italian people with a higher education take a generally positive attitude to the idea of donating biological samples. It contributes to empirical evidence of what Italy's citizens understand about biobanking, and of their willingness to donate samples for research purposes, and also to have their genetic profiles included in a national forensic DNA database. Our findings may have

  14. Evaluation of the Safety and Effectiveness of Add-On Tacrolimus in Patients with Rheumatoid Arthritis Who Failed to Show an Adequate Response to Biological DMARDs: The Interim Results of a Specific Drug Use-Results Survey of Tacrolimus.

    Science.gov (United States)

    Ishida, Kota; Shiraki, Katsuhisa; Yoshiyasu, Takashi

    2015-12-01

    Tacrolimus (TAC) is an immunosuppressive macrolide that blocks T-cell activation by specifically inhibiting calcineurin. TAC was approved in Japan for the treatment of rheumatoid arthritis (RA) in 2005. However, the safety and effectiveness of TAC adding on to biological disease-modifying anti-rheumatic drugs (DMARDs) in the real clinical setting may not be clear enough. We report here the interim results of post marketing surveillance (PMS) of TAC adding on to biological DMARDs in RA patients who failed to show an adequate response to biological DMARDs. Patients who had an inadequate response to biological DMARDs were enrolled. An inadequate response to biological DMARDs was defined as that all of the following conditions were met: a Simplified Disease Activity Index (SDAI) score of >3.3 when TAC was started; both the tender joint count and swollen joint count were the same or increased compared with those at 4-8 weeks prior to TAC; and biological DMARDs were used for at least 8 weeks prior to TAC. This study was conducted in compliance with the ministerial ordinance on "Good Post-Marketing Study Practice" (GPSP). The safety data collection and evaluation for 172 patients and effectiveness data collection and evaluation for 165 patients were reported. The mean age was 61.9 years. Adverse drug reactions occurred in 18 patients. The mean SDAI decreased from 20.1 at baseline to 11.7 at week 24. TAC is well tolerated and effective when added on to biological DMARDs in RA patients who failed to achieve an adequate response to biological DMARDs.

  15. Profile of neuroblastoma detected by mass screening, resected after observation without treatment: results of the Wait and See pilot study.

    Science.gov (United States)

    Oue, Takaharu; Inoue, Masami; Yoneda, Akihiro; Kubota, Akio; Okuyama, Hiroomi; Kawahara, Hisayoshi; Nishikawa, Masanori; Nakayama, Masahiro; Kawa, Keisei

    2005-02-01

    Neuroblastoma (NB) detected by mass screening (MS) usually shows favorable prognosis and sometimes regresses spontaneously. Therefore, the authors started an observation program for these patients to avoid overtreatment. In this study, the authors analyzed the profile of NB resected after observation to elucidate the nature of NB detected by MS. Between 1994 and 2004, 22 NB patients matched the following criteria and entered the observation program after obtaining informed consent: stage I or II, less than 5 cm in diameter, and without involvement of large vessels or organs. If increase in size, elevation of tumor markers, or evidence of metastasis was observed, the tumor was immediately resected. Thirteen (59%) of 22 cases showed spontaneous regression. In the remaining 9 cases, tumors were resected because of parents' request, increase in size, and/or elevation of tumor markers. Four tumors had at least one unfavorable biologic feature, and 3 of them had more than 2. According to Shimada's system, 2 had unfavorable histology. One was diploid tumor, 3 had 1p deletion, and Trk-A expression was low in 4 tumors. All patients survived without evidence of recurrence. The observation program has shown that at least one third of the NB detected by MS regressed spontaneously. On the other hand, MS may detect some cases with unfavorable tumor in early stage, which benefit from screening.

  16. Systematic review and meta-analysis shows a specific micronutrient profile in people with Down Syndrome: Lower blood calcium, selenium and zinc, higher red blood cell copper and zinc, and higher salivary calcium and sodium

    Science.gov (United States)

    Saghazadeh, Amene; Mahmoudi, Maryam; Dehghani Ashkezari, Atefeh; Oliaie Rezaie, Nooshin; Rezaei, Nima

    2017-01-01

    Different metabolic profiles as well as comorbidities are common in people with Down Syndrome (DS). Therefore it is relevant to know whether micronutrient levels in people with DS are also different. This systematic review was designed to review the literature on micronutrient levels in people with DS compared to age and sex-matched controls without DS. We identified sixty nine studies from January 1967 to April 2016 through main electronic medical databases PubMed, Scopus, and Web of knowledge. We carried out meta-analysis of the data on four essential trace elements (Cu, Fe, Se, and Zn), six minerals (Ca, Cl, K, Mg, Na, and P), and five vitamins (vitamin A, B9, B12, D, and E). People with DS showed lower blood levels of Ca (standard mean difference (SMD) = −0.63; 95% confidence interval (CI): −1.16 to −0.09), Se (SMD = -0.99; 95% CI: -1.55 to -0.43), and Zn (SMD = -1.30; 95% CI: -1.75 to -0.84), while red cell levels of Zn (SMD = 1.88; 95% CI: 0.48 to 3.28) and Cu (SMD = 2.77; 95% CI: 1.96 to 3.57) were higher. They had also higher salivary levels of Ca (SMD = 0.85; 95% CI: 0.38 to 1.33) and Na (SMD = 1.04; 95% CI: 0.39 to 1.69). Our findings that micronutrient levels are different in people with DS raise the question whether these differences are related to the different metabolic profiles, the common comorbidities or merely reflect DS. PMID:28422987

  17. Systematic review and meta-analysis shows a specific micronutrient profile in people with Down Syndrome: Lower blood calcium, selenium and zinc, higher red blood cell copper and zinc, and higher salivary calcium and sodium.

    Directory of Open Access Journals (Sweden)

    Amene Saghazadeh

    Full Text Available Different metabolic profiles as well as comorbidities are common in people with Down Syndrome (DS. Therefore it is relevant to know whether micronutrient levels in people with DS are also different. This systematic review was designed to review the literature on micronutrient levels in people with DS compared to age and sex-matched controls without DS. We identified sixty nine studies from January 1967 to April 2016 through main electronic medical databases PubMed, Scopus, and Web of knowledge. We carried out meta-analysis of the data on four essential trace elements (Cu, Fe, Se, and Zn, six minerals (Ca, Cl, K, Mg, Na, and P, and five vitamins (vitamin A, B9, B12, D, and E. People with DS showed lower blood levels of Ca (standard mean difference (SMD = -0.63; 95% confidence interval (CI: -1.16 to -0.09, Se (SMD = -0.99; 95% CI: -1.55 to -0.43, and Zn (SMD = -1.30; 95% CI: -1.75 to -0.84, while red cell levels of Zn (SMD = 1.88; 95% CI: 0.48 to 3.28 and Cu (SMD = 2.77; 95% CI: 1.96 to 3.57 were higher. They had also higher salivary levels of Ca (SMD = 0.85; 95% CI: 0.38 to 1.33 and Na (SMD = 1.04; 95% CI: 0.39 to 1.69. Our findings that micronutrient levels are different in people with DS raise the question whether these differences are related to the different metabolic profiles, the common comorbidities or merely reflect DS.

  18. Validation of Refractivity Profiles Retrieved from FORMOSAT-3/COSMIC Radio Occultation Soundings: Preliminary Results of Statistical Comparisons Utilizing Balloon-Borne Observations

    Directory of Open Access Journals (Sweden)

    Hiroo Hayashi

    2009-01-01

    Full Text Available The GPS radio occultation (RO soundings by the FORMOSAT-3/COSMIC (Taiwan¡¦s Formosa Satellite Misssion #3/Constellation Observing System for Meteorology, Ionosphere and Climate satellites launched in mid-April 2006 are compared with high-resolution balloon-borne (radiosonde and ozonesonde observations. This paper presents preliminary results of validation of the COSMIC RO measurements in terms of refractivity through the troposphere and lower stratosphere. With the use of COSMIC RO soundings within 2 hours and 300 km of sonde profiles, statistical comparisons between the collocated refractivity profiles are erformed for some tropical regions (Malaysia and Western Pacific islands where moisture-rich air is expected in the lower troposphere and for both northern and southern polar areas with a very dry troposphere. The results of the comparisons show good agreement between COSMIC RO and sonde refractivity rofiles throughout the troposphere (1 - 1.5% difference at most with a positive bias generally becoming larger at progressively higher altitudes in the lower stratosphere (1 - 2% difference around 25 km, and a very small standard deviation (about 0.5% or less for a few kilometers below the tropopause level. A large standard deviation of fractional differences in the lowermost troposphere, which reaches up to as much as 3.5 - 5%at 3 km, is seen in the tropics while a much smaller standard deviation (1 - 2% at most is evident throughout the polar troposphere.

  19. The effect of hearing aids and frequency modulation technology on results from the communication profile for the hearing impaired.

    Science.gov (United States)

    Lewis, M Samantha; Valente, Michael; Horn, Jane Enrietto; Crandell, Carl

    2005-04-01

    Hearing impairment has been associated with decline in psychosocial function. Previous investigations have reported that the utilization of hearing aids can ameliorate these reductions in psychosocial function. To date, few investigations have examined the effects of frequency modulation technology on hearing handicap, adjustment to hearing loss, and communicative strategies. The purpose of this investigation was to examine these effects and to compare them to the benefits obtained when using hearing aids alone. Subjects ranged in age from 34 to 81 years and had mean pure-tone thresholds consistent with a bilateral moderate to severe sloping sensorineural hearing loss. All subjects wore hearing aids only and hearing aids plus FM system in a randomized fashion. The Communication Profile for the Hearing Impaired (CPHI) was administered prior to fitting the study devices and once a month for three months in each of the two conditions. A statistically significant difference between device conditions was obtained for the Importance of Communication in Work Situations subscale. Additionally, statistically significant differences over time were noted in several CPHI subscales. Despite statistical significance, none of these results were clinically significant. The implications of these results will be discussed.

  20. GHEP-ISFG collaborative exercise on mixture profiles of autosomal STRs (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03): results and evaluation.

    Science.gov (United States)

    Crespillo, M; Barrio, P A; Luque, J A; Alves, C; Aler, M; Alessandrini, F; Andrade, L; Barretto, R M; Bofarull, A; Costa, S; García, M A; García, O; Gaviria, A; Gladys, A; Gorostiza, A; Hernández, A; Herrera, M; Hombreiro, L; Ibarra, A A; Jiménez, M J; Luque, G M; Madero, P; Martínez-Jarreta, B; Masciovecchio, M V; Modesti, N M; Moreno, F; Pagano, S; Pedrosa, S; Plaza, G; Prat, E; Puente, J; Rendo, F; Ribeiro, T; Sala, A; Santamaría, E; Saragoni, V G; Whittle, M R

    2014-05-01

    One of the main objectives of the Spanish and Portuguese-Speaking Group of the International Society for Forensic Genetics (GHEP-ISFG) is to promote and contribute to the development and dissemination of scientific knowledge in the area of forensic genetics. Due to this fact, GHEP-ISFG holds different working commissions that are set up to develop activities in scientific aspects of general interest. One of them, the Mixture Commission of GHEP-ISFG, has organized annually, since 2009, a collaborative exercise on analysis and interpretation of autosomal short tandem repeat (STR) mixture profiles. Until now, three exercises have been organized (GHEP-MIX01, GHEP-MIX02 and GHEP-MIX03), with 32, 24 and 17 participant laboratories respectively. The exercise aims to give a general vision by addressing, through the proposal of mock cases, aspects related to the edition of mixture profiles and the statistical treatment. The main conclusions obtained from these exercises may be summarized as follows. Firstly, the data show an increased tendency of the laboratories toward validation of DNA mixture profiles analysis following international recommendations (ISO/IEC 17025:2005). Secondly, the majority of discrepancies are mainly encountered in stutters positions (53.4%, 96.0% and 74.9%, respectively for the three editions). On the other hand, the results submitted reveal the importance of performing duplicate analysis by using different kits in order to reduce errors as much as possible. Regarding the statistical aspect (GHEP-MIX02 and 03), all participants employed the likelihood ratio (LR) parameter to evaluate the statistical compatibility and the formulas employed were quite similar. When the hypotheses to evaluate the LR value were locked by the coordinators (GHEP-MIX02) the results revealed a minor number of discrepancies that were mainly due to clerical reasons. However, the GHEP-MIX03 exercise allowed the participants to freely come up with their own hypotheses to

  1. Early adulthood television viewing and cardiometabolic risk profiles in early middle age: results from a population, prospective cohort study.

    Science.gov (United States)

    Stamatakis, E; Hamer, M; Mishra, G D

    2012-02-01

    Little research has been done on the long-term longitudinal associations between markers of sedentary behaviour and health risks. We hypothesised that television (TV) viewing in early to mid-adulthood predicts an adverse cardiometabolic risk factor profile in middle age independently of participation in physical activity. We used prospective data from 5,972 (2,947 men) participants of the 1958 British Birth Cohort study. TV viewing and exercise frequency were obtained at age 23 years. Daily TV viewing and weekly moderate to vigorous physical activity were assessed at age 44 years, as well as HbA(1c), triacylglycerol, total and HDL-cholesterol, systolic and diastolic blood pressure, and waist circumference. We used generalised linear models and multiple linear regression to examine the associations between TV viewing at age 23 years and the cardiometabolic risk markers (including a clustered cardiometabolic risk score) at 44 years, while adjusting for sex, exercise participation and TV viewing at age 44 years, and other potential confounders. In the multivariable models, TV viewing frequency at age 23 years showed positive associations with C-reactive protein (generalised linear model change 12.6%, 95% CI 3.5, 22.8; p = 0.005), fibrinogen (change 1.8%, 95% CI 0.3, 3.3; p = 0.020), waist circumference (coefficient 1.17, 95% CI 0.32, 2.01; p = 0.004), systolic (coefficient 1.44, 95% CI 0.33, 2.54; p = 0.019) and diastolic (coefficient 0.75, 95% CI -0.01, 1.51; p = 0.053) blood pressure, and clustered cardiometabolic risk score (men only, coefficient 0.06, 95% CI 0.01, 0.11; p = 0.038). Adjustments for baseline (age 23 years) BMI attenuated these associations towards null. TV viewing habits in early adulthood are associated with adverse cardiometabolic profiles in early middle adulthood that are independent of TV viewing habits and physical activity in middle age, but not independent of BMI in early adulthood.

  2. Final results of the US humanitarian device exemption study of the low-profile visualized intraluminal support (LVIS) device.

    Science.gov (United States)

    Fiorella, David; Arthur, Adam; Boulos, Alan; Diaz, Orlando; Jabbour, Pascal; Pride, Lee; Turk, Aquilla S; Woo, Henry H; Derdeyn, Colin; Millar, John; Clifton, Andrew

    2016-09-01

    The low-profile visualized intraluminal support (LVIS) device is a new, braided, intracranial microstent designed for stent-assisted coiling. To present the results of a single-arm, prospective, multicenter trial of the LVIS for treatment of wide-necked intracranial aneurysms. 31 patients with unruptured, wide-necked (neck ≥4 mm or dome:neck ratio ≤2) intracranial aneurysms were treated with the LVIS device and bare platinum coils at six US centers (investigational device exemption G110014). Clinical follow-up was conducted at 30 days and 6 months. Angiographic follow-up was performed at 6 months. The primary safety endpoint was any major stroke or death within 30 days or major ipsilateral stroke or neurological death within 6 months. 'Probable benefit' was defined as ≥90% angiographic occlusion at 6 months. An independent core laboratory adjudicated the angiographic results. An independent clinical events committee adjudicated the clinical endpoints. Average aneurysm size was 7.2 mm (SD 3.8) and average neck width was 4.6 mm (SD 1.8). 68% of patients had a dome:neck ratio ≤2. LVIS placement was technically successful in 29/31 patients (93.5%). No primary safety endpoints occurred during the study (0%). No patient had a higher modified Rankin Score at 6 months than at baseline. 26/28 (92.9%) treated aneurysms with 6-month angiographic follow-up demonstrated ≥90% angiographic occlusion. 21/28 (75%) were completely occluded at follow-up. The LVIS device facilitated the coil embolization of wide-necked intracranial aneurysms with high rates of technical success, an excellent safety profile, and very high rates of complete and near-complete occlusion at follow-up. NCT01541254. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

  3. Profiles of Organic Food Consumers in a Large Sample of French Adults: Results from the Nutrinet-Santé Cohort Study

    Science.gov (United States)

    Kesse-Guyot, Emmanuelle; Péneau, Sandrine; Méjean, Caroline; Szabo de Edelenyi, Fabien; Galan, Pilar; Hercberg, Serge; Lairon, Denis

    2013-01-01

    Background Lifestyle, dietary patterns and nutritional status of organic food consumers have rarely been described, while interest for a sustainable diet is markedly increasing. Methods Consumer attitude and frequency of use of 18 organic products were assessed in 54,311 adult participants in the Nutrinet-Santé cohort. Cluster analysis was performed to identify behaviors associated with organic product consumption. Socio-demographic characteristics, food consumption and nutrient intake across clusters are provided. Cross-sectional association with overweight/obesity was estimated using polytomous logistic regression. Results Five clusters were identified: 3 clusters of non-consumers whose reasons differed, occasional (OCOP, 51%) and regular (RCOP, 14%) organic product consumers. RCOP were more highly educated and physically active than other clusters. They also exhibited dietary patterns that included more plant foods and less sweet and alcoholic beverages, processed meat or milk. Their nutrient intake profiles (fatty acids, most minerals and vitamins, fibers) were healthier and they more closely adhered to dietary guidelines. In multivariate models (after accounting for confounders, including level of adherence to nutritional guidelines), compared to those not interested in organic products, RCOP participants showed a markedly lower probability of overweight (excluding obesity) (25≤body mass indexorganic products, a sizeable group in our sample, exhibit specific socio-demographic characteristics, and an overall healthy profile which should be accounted for in further studies analyzing organic food intake and health markers. PMID:24204721

  4. Profiling wind and greenhouse gases by infrared-laser occultation: results from end-to-end simulations in windy air

    Directory of Open Access Journals (Sweden)

    A. Plach

    2015-07-01

    Full Text Available The new mission concept of microwave and infrared-laser occultation between low-Earth-orbit satellites (LMIO is designed to provide accurate and long-term stable profiles of atmospheric thermodynamic variables, greenhouse gases (GHGs, and line-of-sight (l.o.s. wind speed with focus on the upper troposphere and lower stratosphere (UTLS. While the unique quality of GHG retrievals enabled by LMIO over the UTLS has been recently demonstrated based on end-to-end simulations, the promise of l.o.s. wind retrieval, and of joint GHG and wind retrieval, has not yet been analyzed in any realistic simulation setting. Here we use a newly developed l.o.s. wind retrieval algorithm, which we embedded in an end-to-end simulation framework that also includes the retrieval of thermodynamic variables and GHGs, and analyze the performance of both stand-alone wind retrieval and joint wind and GHG retrieval. The wind algorithm utilizes LMIO laser signals placed on the inflection points at the wings of the highly symmetric C18OO absorption line near 4767 cm−1 and exploits transmission differences from a wind-induced Doppler shift. Based on realistic example cases for a diversity of atmospheric conditions, ranging from tropical to high-latitude winter, we find that the retrieved l.o.s. wind profiles are of high quality over the lower stratosphere under all conditions, i.e., unbiased and accurate to within about 2 m s−1 over about 15 to 35 km. The wind accuracy degrades into the upper troposphere due to the decreasing signal-to-noise ratio of the wind-induced differential transmission signals. The GHG retrieval in windy air is not vulnerable to wind speed uncertainties up to about 10 m s−1 but is found to benefit in the case of higher speeds from the integrated wind retrieval that enables correction of wind-induced Doppler shift of GHG signals. Overall both the l.o.s. wind and GHG retrieval results are strongly encouraging towards further development and

  5. Development of a Multivariate Predictive Model to Estimate Ionized Calcium Concentration from Serum Biochemical Profile Results in Dogs.

    Science.gov (United States)

    Danner, J; Ridgway, M D; Rubin, S I; Le Boedec, K

    2017-09-01

    Ionized calcium concentration is the gold standard to assess calcium status in dogs, but measurement is not always available. (1) To predict ionized calcium concentration from biochemical results and compare the diagnostic performance of predicted ionized calcium concentration (piCa) to those of total calcium concentration (tCa) and 2 corrected tCa formulas; and (2) to study the relationship between biochemical results and variation of measured ionized calcium concentration (miCa). A total of 1,719 dogs with both miCa and biochemical profile results available. Cross-sectional study. Using 1,200 dogs, piCa was determined using a multivariate adaptive regression splines model. Its accuracy and performance were tested on the remaining 519 dogs. The final model included creatinine, albumin, tCa, phosphorus, sodium, potassium, chloride, alkaline phosphatase, triglycerides, and age, with tCa, albumin, and chloride having the highest impact on miCa variation. Measured ionized calcium concentration was better correlated with piCa than with tCa and corrected tCa and had higher overall diagnostic accuracy to diagnose hypocalcemia and hypercalcemia, but not significantly for hypercalcemia. For hypercalcemia, piCa was as sensitive (64%) but more specific (99.6%) than tCa and corrected tCa. For hypocalcemia, piCa was more sensitive (21.8%) and as specific (98.4%) as tCa. Positive and negative predictive values of piCa were high for both hypercalcemia (90% and 98%, respectively) and hypocalcemia (70.8% and 87.7%, respectively). Predicted ionized calcium concentration can be obtained from readily available biochemical and patient results and seems more useful than tCa and corrected tCa to assess calcium disorders in dogs when miCa is unavailable. Validation on external data, however, is warranted. Copyright © 2017 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.

  6. Transcutaneous oxygen tension exercise profile. A method for objectively assessing the results after reconstructive peripheral arterial surgery.

    Science.gov (United States)

    Larsen, J F; Christensen, K S; Egeblad, K

    1988-12-01

    Transcutaneous oxygen tension during exercise (TcPo2 exercise profile) was measured on the foot in 10 patients before reconstructive vascular surgery and 9 and 18 months later. The preoperative TcPo2 exercise profiles were abnormal in all 10 patients. In 9 of the patients the reconstructions were successful. In these patients the TcPo2 exercise profiles reverted to normal. In a control group of six healthy persons no significant changes in TcPo2 were observed during the follow-up period of 18 months. The reproducibility determined as the total week-to-week variation of claudicants and controls was 8%. The TcPo2 exercise test is suitable for monitoring the patient after reconstructive surgery, because it is based exclusively on objective data is non-invasive and the measurements are reproducible.

  7. Gestodene and desogestrel do not have a different influence on concentration profiles of ethinylestradiol in women taking oral contraceptives--results of isotope dilution mass spectrometry measurements.

    Science.gov (United States)

    Siekmann, L; Siekmann, A; Bidlingmaier, F; Brill, K; Albring, M

    1998-08-01

    A new method for the quantitative determination of 17alpha-ethinylestradiol-17beta (EE2) in serum is presented here based on the principle of isotope dilution mass spectrometry (IDMS) with [13C]EE2 as internal standard. The technique was used to determine the concentration profiles of EE2 in the serum of female subjects who had taken oral contraceptives with different progestin components. The method has proved to be very reliable with respect to trueness, specificity, precision and detection sensitivity and offers considerable advantages compared with the immunological methods of measurement used to date. Forty-seven female volunteers took two different oral contraceptives containing EE2 combined with different progestins in accordance with a cross-over design. After the administration of 30 microg EE2 combined with 75 microg gestodene (EE2/GSD) or 150 microg desogestrel (EE2/DES), blood samples were taken from the subjects on certain days and in certain previously specified cycles in the course of 12 h after medication. The biometric analysis of the results showed that the concentration profiles of EE2 were in their statistics, significantly equivalent after the administration of either of the two oral contraceptives. The sometimes contradictory results found in former studies after the administration of the different contraceptives were presumably due to the methodological shortcomings of the radioimmunological measurement technique. With the use of the highly accurate and specific technique of IDMS it can now be unequivocally established that the different progestins in the tested oral contraceptives have no influence on the bioavailability of EE2 (area under EE2 serum concentration curves, as usually defined in pharmacokinetics).

  8. Gradient Correlation Method for the Stabilization of Inversion Results of Aerosol Microphysical Properties Retrieved from Profiles of Optical Data

    Directory of Open Access Journals (Sweden)

    Kolgotin Alexei

    2016-01-01

    Full Text Available Correlation relationships between aerosol microphysical parameters and optical data are investigated. The results show that surface-area concentrations and extinction coefficients are linearly correlated with a correlation coefficient above 0.99 for arbitrary particle size distribution. The correlation relationships that we obtained can be used as constraints in our inversion of optical lidar data. Simulation studies demonstrate a significant stabilization of aerosol microphysical data products if we apply the gradient correlation method in our traditional regularization technique.

  9. Secondary Metabolic Profiles of Two Cultivars of Piper nigrum (Black Pepper Resulting from Infection by Fusarium solani f. sp. piperis

    Directory of Open Access Journals (Sweden)

    Shirlley F. M. da Luz

    2017-12-01

    Full Text Available Bragantina and Cingapura are the main black pepper (Piper nigrum L. cultivars and the Pará state is the largest producer in Brazil with about 90% of national production, representing the third largest production in the world. The infection of Fusarium solani f. sp. piperis, the causal agent of Fusarium disease in black pepper, was monitored on the cultivars Bragantina (susceptible and Cingapura (tolerant, during 45 days’ post infection (dpi. Gas Chromatography-Mass spectrometry (GC-MS analysis of the volatile concentrates of both cultivars showed that the Bragantina responded with the production of higher contents of α-bisabolol at 21 dpi and a decrease of elemol, mostly at 30 dpi; while Cingapura displayed an decrease of δ-elemene production, except at 15 dpi. The phenolic content determined by the Folin Ciocalteu method showed an increase in the leaves of plants inoculated at 7 dpi (Bragantina and 7–15 dpi (Cingapura; in the roots, the infection caused a phenolic content decrease in Bragantina cultivar at 45 dpi and an increase in the Cingapura cultivar at 15, 30 and 45 dpi. High Performance Liquid Chromatography-Mass spectrometry (HPLC-MS analysis of the root extracts showed a qualitative variation of alkamides during infection. The results indicated that there is a possible relationship between secondary metabolites and tolerance against phytopathogens.

  10. Secondary Metabolic Profiles of Two Cultivars of Piper nigrum (Black Pepper) Resulting from Infection by Fusarium solani f. sp. piperis.

    Science.gov (United States)

    da Luz, Shirlley F M; Yamaguchi, Lydia F; Kato, Massuo J; de Lemos, Oriel F; Xavier, Luciana P; Maia, José Guilherme S; Ramos, Alessandra de R; Setzer, William N; da Silva, Joyce Kelly do R

    2017-12-07

    Bragantina and Cingapura are the main black pepper ( Piper nigrum L.) cultivars and the Pará state is the largest producer in Brazil with about 90% of national production, representing the third largest production in the world. The infection of Fusarium solani f. sp. piperis , the causal agent of Fusarium disease in black pepper, was monitored on the cultivars Bragantina (susceptible) and Cingapura (tolerant), during 45 days' post infection (dpi). Gas Chromatography-Mass spectrometry (GC-MS) analysis of the volatile concentrates of both cultivars showed that the Bragantina responded with the production of higher contents of α-bisabolol at 21 dpi and a decrease of elemol, mostly at 30 dpi; while Cingapura displayed an decrease of δ-elemene production, except at 15 dpi. The phenolic content determined by the Folin Ciocalteu method showed an increase in the leaves of plants inoculated at 7 dpi (Bragantina) and 7-15 dpi (Cingapura); in the roots, the infection caused a phenolic content decrease in Bragantina cultivar at 45 dpi and an increase in the Cingapura cultivar at 15, 30 and 45 dpi. High Performance Liquid Chromatography-Mass spectrometry (HPLC-MS) analysis of the root extracts showed a qualitative variation of alkamides during infection. The results indicated that there is a possible relationship between secondary metabolites and tolerance against phytopathogens.

  11. [Cardiovascular risk profile and lifestyle habits in a cohort of Italian cardiologists. Results of the SOCRATES survey].

    Science.gov (United States)

    Faggianoi, Pompilio; Temporelli, Pier Luigi; Zito, Giovanni; Bovenzi, Francesco; Colivicchi, Furio; Fattirolli, Francesco; Greco, Cesare; Mureddu, Gianfrancesco; Riccio, Carmine; Scherillo, Marino; Uguccioni, Massimo; Faden, Giacomo

    2013-09-01

    To offer a snapshot of the personal health habits of Italian cardiologists, the Survey on Cardiac Risk Profile and Lifestyle Habits in a Cohort of Italian Cardiologists (SOCRATES) study was undertaken. Cardiologists' cardiovascular profile and lifestyle habits are poorly known worldwide. A Web-based electronic self-reported survey, accessible through a dedicated website, was used for data entry, and data were transferred via the web to a central database. The survey was divided in 4 sections: baseline characteristics, medical illnesses and traditional cardiovascular risk factors, lifestyle habits and selected medication use. The e-mail databases of three national scientific societies were used to survey a large and representative sample of Italian cardiologists. During the 3-month period of the survey, 1770 out of the 5240 cardiologists contacted (33.7%) completed and returned one or more sections of the questionnaire. More than 49% of the participants had 1 out of 5 classical risk factors (e.g. hypertension, hypercholesterolemia, active smoking, diabetes and previous vascular events). More than 28% of respondents had 2 to 5 risk factors and only 22.1% had none and therefore, according to age and sex, could be considered at low-intermediate risk. Despite the reported risk factors, more than 90% of cardiologists had a self-reported risk perception quantified as mild, such as low or intermediate. Furthermore, overweight/obesity, physical inactivity and stress at work or at home were commonly reported, as well as a limited use of cardiovascular drugs, such as statins or aspirin. The average cardiovascular profile of Italian cardiologist is unlikely to be considered ideal or even favorable according to recent statements and guidelines regarding cardiovascular risk. Thus, there is a large room for improvement and a need for education and intervention.

  12. Bile acid profiles over 5 years after gastric bypass and duodenal switch: results from a randomized clinical trial.

    Science.gov (United States)

    Risstad, Hilde; Kristinsson, Jon A; Fagerland, Morten W; le Roux, Carel W; Birkeland, Kåre I; Gulseth, Hanne L; Thorsby, Per M; Vincent, Royce P; Engström, My; Olbers, Torsten; Mala, Tom

    2017-09-01

    Bile acids have been proposed as key mediators of the metabolic effects after bariatric surgery. Currently no reports on bile acid profiles after duodenal switch exist, and long-term data after gastric bypass are lacking. To investigate bile acid profiles up to 5 years after Roux-en-Y gastric bypass and biliopancreatic diversion with duodenal switch and to explore the relationship among bile acids and weight loss, lipid profile, and glucose metabolism. Two Scandinavian University Hospitals. We present data from a randomized clinical trial of 60 patients with body mass index 50-60 kg/m2 operated with gastric bypass or duodenal switch. Repeated measurements of total and individual bile acids from fasting serum during 5 years after surgery were performed. Mean concentrations of total bile acids increased from 2.3 µmol/L (95% confidence interval [CI], -.1 to 4.7) at baseline to 5.9 µmol/L (3.5-8.3) 5 years after gastric bypass and from 1.0 µmol/L (95% CI, -1.4 to 3.5) to 9.5 µmol/L (95% CI, 7.1-11.9) after duodenal switch; mean between-group difference was -4.8 µmol/L (95% CI, -9.3 to -.3), P = .036. Mean concentrations of primary bile acids increased more after duodenal switch, whereas secondary bile acids increased proportionally across the groups. Higher levels of total bile acids at 5 years were associated with lower body mass index, greater weight loss, and lower total cholesterol. Total bile acid concentrations increased substantially over 5 years after both gastric bypass and duodenal switch, with greater increases in total and primary bile acids after duodenal switch. (Surg Obes Relat Dis 2017;0:000-000.) © 2017 American Society for Metabolic and Bariatric Surgery. All rights reserved. Copyright © 2017 American Society for Bariatric Surgery. Published by Elsevier Inc. All rights reserved.

  13. Evaluation of the aerosol vertical distribution in global aerosol models through comparison against CALIOP measurements: AeroCom phase II results: AEROSOL PROFILES IN AEROCOM II GCM

    Energy Technology Data Exchange (ETDEWEB)

    Koffi, Brigitte [European Commission, Joint Research Centre, Institute for Environment and Sustainability, Ispra Italy; Schulz, Michael [Norwegian Meteorological Institute, Oslo Norway; Bréon, François-Marie [Laboratoire des Sciences du Climat et de l' Environnement, Gif-sur-Yvette France; Dentener, Frank [European Commission, Joint Research Centre, Institute for Environment and Sustainability, Ispra Italy; Steensen, Birthe Marie [Norwegian Meteorological Institute, Oslo Norway; Griesfeller, Jan [Norwegian Meteorological Institute, Oslo Norway; Winker, David [NASA Langley Research Center, MS/475, Hampton Virginia USA; Balkanski, Yves [Laboratoire des Sciences du Climat et de l' Environnement, Gif-sur-Yvette France; Bauer, Susanne E. [Center for Climate Systems Research, Columbia University, New York New York USA; NASA Goddard Institute for Space Studies, New York New York USA; Bellouin, Nicolas [Department of Meteorology, University of Reading, Reading UK; Berntsen, Terje [Department of Geosciences, University of Oslo, Oslo Norway; Center for International Climate and Environmental Research-Oslo (CICERO), Oslo Norway; Bian, Huisheng [NASA Goddard Space Flight Center, Greenbelt Maryland USA; Joint Center for Earth Systems Technology, University of Maryland Baltimore County, Baltimore Country Maryland USA; Chin, Mian [NASA Goddard Space Flight Center, Greenbelt Maryland USA; Diehl, Thomas [European Commission, Joint Research Centre, Institute for Environment and Sustainability, Ispra Italy; Easter, Richard [Pacific Northwest National Laboratory, Richland Washington USA; Ghan, Steven [Pacific Northwest National Laboratory, Richland Washington USA; Hauglustaine, Didier A. [Laboratoire des Sciences du Climat et de l' Environnement, Gif-sur-Yvette France; Iversen, Trond [Norwegian Meteorological Institute, Oslo Norway; Department of Geosciences, University of Oslo, Oslo Norway; Kirkevåg, Alf [Norwegian Meteorological Institute, Oslo Norway; Liu, Xiaohong [Pacific Northwest National Laboratory, Richland Washington USA; Now at University of Wyoming, Laramie Wyoming USA; Lohmann, Ulrike [ETH-Zentrum, Zürich Switzerland; Myhre, Gunnar [Center for International Climate and Environmental Research-Oslo (CICERO), Oslo Norway; Rasch, Phil [NASA Goddard Space Flight Center, Greenbelt Maryland USA; Seland, Øyvind [Norwegian Meteorological Institute, Oslo Norway; Skeie, Ragnhild B. [Center for International Climate and Environmental Research-Oslo (CICERO), Oslo Norway; Steenrod, Stephen D. [NASA Goddard Space Flight Center, Greenbelt Maryland USA; Stier, Philip [Department of Physics, University of Oxford, Oxford UK; Tackett, Jason [Science Systems and Applications, Inc., Hampton Virginia USA; Takemura, Toshihiko [Research Institute for Applied Mechanics, Kyushu University, Fukuoka Japan; Tsigaridis, Kostas [Center for Climate Systems Research, Columbia University, New York New York USA; NASA Goddard Institute for Space Studies, New York New York USA; Vuolo, Maria Raffaella [Laboratoire des Sciences du Climat et de l' Environnement, Gif-sur-Yvette France; Now at National Institute for Agronomic Research, Thiverval-Grignon France; Yoon, Jinho [Pacific Northwest National Laboratory, Richland Washington USA; Now at Gwangju Institute of Science and Technology, Gwangju Korea; Zhang, Kai [Pacific Northwest National Laboratory, Richland Washington USA; Max Planck Institute for Meteorology, Hamburg Germany

    2016-06-27

    The ability of eleven models in simulating the aerosol vertical distribution from regional to global scales, as part of the second phase of the AeroCom model inter-comparison initiative (AeroCom II) is assessed and compared to results of the first phase. The evaluation is performed using a global monthly gridded dataset of aerosol extinction profiles built on purpose from the CALIOP (Cloud-Aerosol Lidar with Orthogonal Polarization) Layer Product 3.01. Results over 12 sub-continental regions show that five models improved whereas three degraded in reproducing the Zα 0-6 km mean extinction height diagnostic, which is computed over the 0-6 km altitude range for each studied region and season. While the models’ performance remains highly variable, it has generally improved in terms of inter-regional diversity and seasonality. The biases in Zα 0-6 km have notably decreased in the U.S. and European industrial and downwind maritime regions, whereas the timing of the Zα 0-6 km peak season has improved for all but two models. However, most of the models now show a Zα 0-6 km underestimation over land, notably in the dust and biomass burning regions in Asia and Africa. At global scale, the AeroCom II models better reproduce the Zα 0-6 km latitudinal variability over ocean than over land. Hypotheses for the (changes in the) the performance of the individual models and for the inter-model diversity are discussed. We also provide an analysis of the CALIOP limitations and uncertainties that can contribute to the differences between the simulations and observations.

  14. Greenhouse gas profiling by infrared-laser and microwave occultation: retrieval algorithm and demonstration results from end-to-end simulations

    Science.gov (United States)

    Proschek, V.; Kirchengast, G.; Schweitzer, S.

    2011-10-01

    Measuring greenhouse gas (GHG) profiles with global coverage and high accuracy and vertical resolution in the upper troposphere and lower stratosphere (UTLS) is key for improved monitoring of GHG concentrations in the free atmosphere. In this respect a new satellite mission concept adding an infrared-laser part to the already well studied microwave occultation technique exploits the joint propagation of infrared-laser and microwave signals between Low Earth Orbit (LEO) satellites. This synergetic combination, referred to as LEO-LEO microwave and infrared-laser occultation (LMIO) method, enables to retrieve thermodynamic profiles (pressure, temperature, humidity) and accurate altitude levels from the microwave signals and GHG profiles from the simultaneously measured infrared-laser signals. However, due to the novelty of the LMIO method, a retrieval algorithm for GHG profiling is not yet available. Here we introduce such an algorithm for retrieving GHGs from LEO-LEO infrared-laser occultation (LIO) data, applied as a second step after retrieving thermodynamic profiles from LEO-LEO microwave occultation (LMO) data. We thoroughly describe the LIO retrieval algorithm and unveil the synergy with the LMO-retrieved pressure, temperature, and altitude information. We furthermore demonstrate the effective independence of the GHG retrieval results from background (a priori) information in discussing demonstration results from LMIO end-to-end simulations for a representative set of GHG profiles, including carbon dioxide (CO2), water vapor (H2O), methane (CH4), and ozone (O3). The GHGs except for ozone are well retrieved throughout the UTLS, while ozone is well retrieved from about 10 km to 15 km upwards, since the ozone layer resides in the lower stratosphere. The GHG retrieval errors are generally smaller than 1% to 3% r.m.s., at a vertical resolution of about 1 km. The retrieved profiles also appear unbiased, which points to the climate benchmarking capability of the LMIO

  15. Genome-wide gene expression profiling and a forward genetic screen show that differential expression of the sodium ion transporter Ena21 contributes to the differential tolerance of Candida albicans and Candida dubliniensis to osmotic stress.

    LENUS (Irish Health Repository)

    Enjalbert, Brice

    2009-04-01

    Candida albicans is more pathogenic than Candida dubliniensis. However, this disparity in virulence is surprising given the high level of sequence conservation and the wide range of phenotypic traits shared by these two species. Increased sensitivity to environmental stresses has been suggested to be a possible contributory factor to the lower virulence of C. dubliniensis. In this study, we investigated, in the first comparison of C. albicans and C. dubliniensis by transcriptional profiling, global gene expression in each species when grown under conditions in which the two species exhibit differential stress tolerance. The profiles revealed similar core responses to stresses in both species, but differences in the amplitude of the general transcriptional responses to thermal, salt and oxidative stress. Differences in the regulation of specific stress genes were observed between the two species. In particular, ENA21, encoding a sodium ion transporter, was strongly induced in C. albicans but not in C. dubliniensis. In addition, ENA21 was identified in a forward genetic screen for C. albicans genomic sequences that increase salt tolerance in C. dubliniensis. Introduction of a single copy of CaENA21 was subsequently shown to be sufficient to confer salt tolerance upon C. dubliniensis.

  16. Adverse lipid profile is not associated with relapse risk in MS: results from an observational cohort study.

    Science.gov (United States)

    Tettey, Prudence; Simpson, Steve; Taylor, Bruce; Blizzard, Leigh; Ponsonby, Anne-Louise; Dwyer, Terence; Kostner, Karam; van der Mei, Ingrid

    2014-05-15

    There is increasing evidence that serum lipids and apolipoproteins may be associated with multiple sclerosis (MS) clinical course. To investigate the associations between serum lipids, apolipoproteins, body mass index and relapse in MS. A prospective cohort of 141 participants with relapsing-remitting MS was followed from 2002 to 2005. Serum lipid and apolipoprotein levels were measured biannually, and body mass index at baseline. The association with hazard of relapse was assessed using survival analysis. Neither body mass index nor any of the lipid-related measures were associated with the hazard of relapse. Serum lipid profile and body mass index are not associated with the hazard of relapse in MS. Copyright © 2014 Elsevier B.V. All rights reserved.

  17. Gender differences in symptomatic profiles of depression: results from the São Paulo Megacity Mental Health Survey.

    Science.gov (United States)

    Alexandrino-Silva, Clóvis; Wang, Yuan-Pang; Carmen Viana, Maria; Bulhões, Rodrigo S; Martins, Sílvia S; Andrade, Laura Helena

    2013-05-01

    Few studies have investigated symptomatic subtypes of depression and their correlates by gender. Data are from the São Paulo Megacity Mental Health Survey. Symptom profiles of 1207 subjects (864 women; 343 men) based upon symptoms of the worst depressive episode in lifetime were examined through latent class analysis. Correlates of gender-specific latent classes were analyzed by logistic regression. For both men and women, a 3-class model was the best solution. A mild class was found in both genders (41.1% in women; 40.1% in men). Gender differences appeared in the most symptomatic classes. In women, they were labeled melancholic (39.3%) and atypical (19.5%), differing among each other in somatic/vegetative symptoms. The melancholic class presented inhibition and eating/sleeping symptoms in the direction of decreasing, whereas the atypical class had increased appetite/weight, and hypersomnia. For men, symptoms that differentiate the two most symptomatic classes were related to psychomotor activity: a melancholic/psychomotor retarded (40.4%) and agitated depression (19.6%). The highest between-class proportion of agitation and racing thoughts was found among men in the agitated class, with similarity to bipolar mixed state. Analyses were restricted to those who endorsed questions about their worst lifetime depressive episode; the standardized assessment by lay interviewers; the small male sample size. The construct of depression of current classifications is heterogeneous at the symptom level, where gender different subtypes can be identified. These symptom profiles have potential implications for the nosology and the therapeutics of depression. Copyright © 2012 Elsevier B.V. All rights reserved.

  18. Pharmacogenetic profile of a South Portuguese population: results from the pilot study of the European Health Examination Survey in Portugal.

    Science.gov (United States)

    Gaio, Vânia; Picanço, Isabel; Nunes, Baltazar; Fernandes, Aida; Mendonça, Francisco; Horta Correia, Filomena; Beleza, Álvaro; Gil, Ana Paula; Bourbon, Mafalda; Vicente, Astrid; Matias Dias, Carlos; Barreto da Silva, Marta

    2015-01-01

    The genetic inter-individual variability of drug response can lead to therapeutic failure or adverse drug reactions (ADRs). The aims of this study were to assess the pharmacogenetic profile of a South Portuguese population according to established dosing guidelines for commonly prescribed drugs and to compare it with that of previously genotyped populations. A cross-sectional study was developed in the context of the Portuguese Component of the European Health Examination Survey (EHES). A total of 47 pharmacogenetically relevant variants in 23 different genes were genotyped in 208 participants. Allelic and genotypic frequencies were calculated, and the pharmacogenetic profile of the participants was defined. A comparative analysis was conducted through electronic database search. Pairwise Fst calculations were performed to assess the genetic distance between populations. We found a significant small differentiation between the Portuguese regional populations regarding CYP2C9 rs1057910, CYP2D6 rs3892097, MTHFR rs1801133 and F5 rs6025. When consid-ering 4 HapMap populations, ADH1B rs2066702, ADH1B rs1229984, NAT2 rs1799931 and VKORC1 rs9923231 displayed a significant population differentiation. We found that 18.9% of the participants are intermediate or poor metabolizers for at least 3 drugs simultaneously and that 84.6% of the participants have at least one therapeutic failure or ADR risk allele for the considered drugs. There is a high prevalence of risk alleles associated with an altered drug metabolism regarding drugs largely used by the South Portuguese population. This knowledge contributes to the prediction of their clinical efficacy and/or toxicity, optimizing therapeutic response while improving cost-effectiveness. © 2015 S. Karger AG, Basel.

  19. Laser-assisted penetrating keratoplasty: 1-year results in patients using a laser-welded anvil-profiled graft.

    Science.gov (United States)

    Canovetti, Annalisa; Malandrini, Alex; Lenzetti, Ivo; Rossi, Francesca; Pini, Roberto; Menabuoni, Luca

    2014-10-01

    To describe a new laser-assisted penetrating keratoplasty technique combining a femtosecond anvil-like trephination pattern with the laser welding procedure. Cohort prospective study with 1 year of follow-up (June 2011 through January 2013). This study was performed at Misericordia e Dolce Public Hospital, Prato, Italy. Twenty-four eyes of 22 patients underwent penetrating keratoplasty: 4 had granular dystrophy, 12 had keratoconus in its final stages, 3 had post-herpetic leukoma, and 5 had corneal scars. A femtosecond laser was used to create anvil-profiled cuts in donor and recipient corneas. Diode laser welding was performed, supporting standard suturing. All patients were evaluated for corrected distance visual acuity, pachymetry, manifest astigmatism, and endothelial cell density after 1, 3, 6, and 12 months. Mean ± standard deviation postoperative corrected visual acuity was 0.48 ± 0.23 logarithm of the minimal angle of resolution (logMAR), 0.30 ± 0.18 logMAR, 0.18 ± 0.13 logMAR, and 0.13 ± 0.16 logMAR at 1, 3, 6, and 12 months, respectively. At the same follow-up times, mean pachymetry was 537 ± 57 μm, 533 ± 74 μm, 528 ± 72 μm, and 529 ± 58 μm, respectively; and mean endothelial cell density was 1945 ± 371 cells/mm(2), 1881 ± 410 cells/mm(2), 1781 ± 401 cells/mm(2), and 1730 ± 376 cells/mm(2), respectively. Mean manifest and topographic postoperative astigmatism were: 3.6 ± 2.5 diopters (D) and 4.65 ± 2.57 D at 1 month, 2.93 ± 2.34 D and 4.79 ± 2.85 D at 3 months, 2.82 ± 1.75 D and 3.44 ± 2.28 D at 6 months, and 2.08 ± 1.25 D and 2.73 ± 2.01 D at 12 months, respectively. All surgical operations were successful and without intraoperative complications. The use of the anvil trephination profile was effective for performing laser-assisted penetrating keratoplasty. The large donor-recipient interface enables the laser welding procedure and good preservation of the recipient's endothelial cell pool. Copyright © 2014 Elsevier Inc. All

  20. Dangers resulting from DNA profiling of biological materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT with regard to forensic genetic analysis

    Directory of Open Access Journals (Sweden)

    Renata Jacewicz

    2016-07-01

    Full Text Available The study documents the risk that comes with DNA analysis of materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT in forensic genetics. DNA chimerism was studied in 30 patients after allo-HSCT, based on techniques applied in contemporary forensic genetics, i.e. real-time PCR and multiplex PCR-STR with the use of autosomal DNA as well as Y-DNA markers. The results revealed that the DNA profile of the recipient’s blood was identical with the donor’s in the majority of cases. Therefore, blood analysis can lead to false conclusions in personal identification as well as kinship analysis. An investigation of buccal swabs revealed a mixture of DNA in the majority of recipients. Consequently, personal identification on the basis of stain analysis of the same origin may be impossible. The safest (but not ideal material turned out to be the hair root. Its analysis based on autosomal DNA revealed 100% of the recipient’s profile. However, an analysis based on Y-chromosome markers performed in female allo-HSCT recipients with male donors demonstrated the presence of donor DNA in hair cells – similarly to the blood and buccal swabs. In the light of potential risks arising from DNA profiling of biological materials derived from persons after allotransplantation in judicial aspects, certain procedures were proposed to eliminate such dangers. The basic procedures include abandoning the approach based exclusively on blood collection, both for kinship analysis and personal identification; asking persons who are to be tested about their history of allo-HSCT before sample collection and profile entry in the DNA database, and verification of DNA profiling based on hair follicles in uncertain cases.

  1. Dangers resulting from DNA profiling of biological materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) with regard to forensic genetic analysis.

    Science.gov (United States)

    Jacewicz, R; Lewandowski, K; Rupa-Matysek, J; Jędrzejczyk, M; Berent, J

    The study documents the risk that comes with DNA analysis of materials derived from patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT) in forensic genetics. DNA chimerism was studied in 30 patients after allo-HSCT, based on techniques applied in contemporary forensic genetics, i.e. real-time PCR and multiplex PCR-STR with the use of autosomal DNA as well as Y-DNA markers. The results revealed that the DNA profile of the recipient's blood was identical with the donor's in the majority of cases. Therefore, blood analysis can lead to false conclusions in personal identification as well as kinship analysis. An investigation of buccal swabs revealed a mixture of DNA in the majority of recipients. Consequently, personal identification on the basis of stain analysis of the same origin may be impossible. The safest (but not ideal) material turned out to be the hair root. Its analysis based on autosomal DNA revealed 100% of the recipient's profile. However, an analysis based on Y-chromosome markers performed in female allo-HSCT recipients with male donors demonstrated the presence of donor DNA in hair cells - similarly to the blood and buccal swabs. In the light of potential risks arising from DNA profiling of biological materials derived from persons after allotransplantation in judicial aspects, certain procedures were proposed to eliminate such dangers. The basic procedures include abandoning the approach based exclusively on blood collection, both for kinship analysis and personal identification; asking persons who are to be tested about their history of allo-HSCT before sample collection and profile entry in the DNA database, and verification of DNA profiling based on hair follicles in uncertain cases.

  2. Tropospheric ozone profiles by DIAL at Maïdo Observatory (Reunion Island): system description, instrumental performance and result comparison with ozone external data set

    Science.gov (United States)

    Duflot, Valentin; Baray, Jean-Luc; Payen, Guillaume; Marquestaut, Nicolas; Posny, Francoise; Metzger, Jean-Marc; Langerock, Bavo; Vigouroux, Corinne; Hadji-Lazaro, Juliette; Portafaix, Thierry; De Mazière, Martine; Coheur, Pierre-Francois; Clerbaux, Cathy; Cammas, Jean-Pierre

    2017-09-01

    In order to recognize the importance of ozone (O3) in the troposphere and lower stratosphere in the tropics, a DIAL (differential absorption lidar) tropospheric O3 lidar system (LIO3TUR) was developed and installed at the Université de la Réunion campus site (close to the sea) on Reunion Island (southern tropics) in 1998. From 1998 to 2010, it acquired 427 O3 profiles from the low to the upper troposphere and has been central to several studies. In 2012, the system was moved up to the new Maïdo Observatory facility (2160 m a.m.s.l. - metres above mean sea level) where it started operation in February 2013. The current system (LIO3T) configuration generates a 266 nm beam obtained with the fourth harmonic of a Nd:YAG laser sent into a Raman cell filled up with deuterium (using helium as buffer gas), generating the 289 and 316 nm beams to enable the use of the DIAL method for O3 profile measurements. The optimal range for the actual system is 6-19 km a.m.s.l., depending on the instrumental and atmospheric conditions. For a 1 h integration time, vertical resolution varies from 0.7 km at 6 km a.m.s.l. to 1.3 km at 19 km a.m.s.l., and mean uncertainty within the 6-19 km range is between 6 and 13 %. Comparisons with eight electrochemical concentration cell (ECC) sondes simultaneously launched from the Maïdo Observatory show good agreement between data sets with a 6.8 % mean absolute relative difference (D) between 6 and 17 km a.m.s.l. (LIO3T lower than ECC). Comparisons with 37 ECC sondes launched from the nearby Gillot site during the daytime in a ±24 h window around lidar shooting result in a 9.4 % D between 6 and 19 km a.m.s.l. (LIO3T lower than ECC). Comparisons with 11 ground-based Network for Detection of Atmospheric Composition Change (NDACC) Fourier transform infrared (FTIR) spectrometer measurements acquired during the daytime in a ±24 h window around lidar shooting show good agreement between data sets with a D of 11.8 % for the 8.5-16 km partial column

  3. Influence of the fluid structure on the binding potential: Comparing liquid drop profiles from density functional theory with results from mesoscopic theory.

    Science.gov (United States)

    Hughes, Adam P; Thiele, Uwe; Archer, Andrew J

    2017-02-14

    For a film of liquid on a solid surface, the binding potential g(h) gives the free energy as a function of the film thickness h and also the closely related (structural) disjoining pressure Π=-∂g/∂h. The wetting behaviour of the liquid is encoded in the binding potential and the equilibrium film thickness corresponds to the value at the minimum of g(h). Here, the method we developed in the work of Hughes et al. [J. Chem. Phys. 142, 074702 (2015)], and applied with a simple discrete lattice-gas model, is used with continuum density functional theory (DFT) to calculate the binding potential for a Lennard-Jones fluid and other simple liquids. The DFT used is based on fundamental measure theory and so incorporates the influence of the layered packing of molecules at the surface and the corresponding oscillatory density profile. The binding potential is frequently input in mesoscale models from which liquid drop shapes and even dynamics can be calculated. Here we show that the equilibrium droplet profiles calculated using the mesoscale theory are in good agreement with the profiles calculated directly from the microscopic DFT. For liquids composed of particles where the range of the attraction is much less than the diameter of the particles, we find that at low temperatures g(h) decays in an oscillatory fashion with increasing h, leading to highly structured terraced liquid droplets.

  4. Influence of the fluid structure on the binding potential: Comparing liquid drop profiles from density functional theory with results from mesoscopic theory

    Science.gov (United States)

    Hughes, Adam P.; Thiele, Uwe; Archer, Andrew J.

    2017-02-01

    For a film of liquid on a solid surface, the binding potential g(h) gives the free energy as a function of the film thickness h and also the closely related (structural) disjoining pressure Π =-∂g /∂h . The wetting behaviour of the liquid is encoded in the binding potential and the equilibrium film thickness corresponds to the value at the minimum of g(h). Here, the method we developed in the work of Hughes et al. [J. Chem. Phys. 142, 074702 (2015)], and applied with a simple discrete lattice-gas model, is used with continuum density functional theory (DFT) to calculate the binding potential for a Lennard-Jones fluid and other simple liquids. The DFT used is based on fundamental measure theory and so incorporates the influence of the layered packing of molecules at the surface and the corresponding oscillatory density profile. The binding potential is frequently input in mesoscale models from which liquid drop shapes and even dynamics can be calculated. Here we show that the equilibrium droplet profiles calculated using the mesoscale theory are in good agreement with the profiles calculated directly from the microscopic DFT. For liquids composed of particles where the range of the attraction is much less than the diameter of the particles, we find that at low temperatures g(h) decays in an oscillatory fashion with increasing h, leading to highly structured terraced liquid droplets.

  5. Multi-domain simulation of transient junction temperatures and resulting stress-strain behavior of power switches for long-term mission profiles

    Energy Technology Data Exchange (ETDEWEB)

    Drofenik, U.; Kovacevic, I.; Kolar, J. W. [Swiss Federal Institute of Technology, Power Electronic Systems Laboratory, Zuerich (Switzerland); Schmidt, R. [ABB Switzerland Ltd., Corporate Research, Baden-Daettwil (Switzerland)

    2008-07-01

    For lifetime estimation of power converters in traction applications, one method is to calculate numerically the stress-strain hysteresis curves of the interfaces silicon-solder-DCB and/or DCB-solder-baseplate inside the power modules. This can only be achieved if the transient junction temperatures in these layers are known for a defined mission profile. Therefore, one has to couple circuit simulation with thermal simulation and stress-strain computation. The second challenge of this problem is to perform this transient simulation taking into account switching losses in the {mu}s-range for mission profiles over a couple of minutes. In this paper we employ a new multi-domain simulation software to achieve results with reasonable computational effort. (author)

  6. Showing Value (Editorial

    Directory of Open Access Journals (Sweden)

    Denise Koufogiannakis

    2009-06-01

    librarians on student achievement. Todd notes, “If we do not show value, we will not have a future. Evidence-based practice is not about the survival of school librarians, it’s about the survival of our students” (40. In this issue we feature school libraries and their connection to evidence based practice. Former Editor-in-Chief, Lindsay Glynn, began putting the wheels in motion for this feature almost a year ago. She invited Carol Gordon and Ross Todd to act as guest editors of the section, drawing upon their contacts and previous work in this field. The result is an issue with five feature articles exploring different aspects of the connection between school libraries and evidence based practice, from the theoretical to the practical. In addition, there is a thought-provoking Commentary by David Loertscher, asking whether we need the evolutionary model of evidence based practice, or something more revolutionary!In addition to the Feature section, we have a well-rounded issue with articles on the topics of library human resources, and the development of a scholars’ portal. As well, there are a record 10 evidence summaries and our educational EBL101 column. I hope there is something for everyone in this issue of EBLIP – enjoy, and see you soon in Stockholm!

  7. Development of a Multivariate Predictive Model to Estimate Ionized Calcium Concentration from Serum Biochemical Profile Results in Dogs

    OpenAIRE

    Danner, J.; Ridgway, M.D.; Rubin, S I; Le Boedec, K.

    2017-01-01

    Background Ionized calcium concentration is the gold standard to assess calcium status in dogs, but measurement is not always available. Objectives (1) To predict ionized calcium concentration from biochemical results and compare the diagnostic performance of predicted ionized calcium concentration (piCa) to those of total calcium concentration (tCa) and 2 corrected tCa formulas; and (2) to study the relationship between biochemical results and variation of measured ionized calcium concentrat...

  8. Administration of a new diabetes-specific enteral formula results in an improved 24h glucose profile in type 2 diabetic patients.

    Science.gov (United States)

    Ceriello, Antonio; Lansink, Mirian; Rouws, Carlette H F C; van Laere, Katrien M J; Frost, Gary S

    2009-06-01

    Study the effect of several boluses of a new diabetes-specific formula (DSF) during the day on 24h glucose profile. In this randomized, controlled, double-blind, cross-over study 12 ambulatory type 2 diabetic patients were included. Subjects received a new DSF and an isocaloric standard fibre-containing formula (SF) while continuing their anti-diabetic medication. Subjects received 100% of their calculated daily energy requirements as bolus feeding every 3h (5 times/day, starting at 8.00 a.m.+/-1h). Glucose profiles were significantly better after administration of DSF compared with SF determined as mean glucose concentration (+/-SEM) (8.7+/-0.5 versus 9.6+/-0.6 mmol/L, p<0.05 during 24h; 9.4+/-0.6 versus 10.7+/-0.6 mmol/L, p<0.001 during daytime) or as incremental area under the curve during daytime (-44%; p<0.05). Subjects receiving DSF experienced less hyperglycaemic time over 24h (-26%; p<0.05) and during daytime (-30%; p<0.05). Furthermore, lower individual and mean (delta) peak glucose levels were found (p<0.05). No clinically relevant differences in gastrointestinal tolerance were observed. Using DSF resulted in significantly better 24h and postprandial glucose profiles than fibre-containing SF after bolus administration and may therefore help to improve glycaemic control in diabetic patients.

  9. Geological affinity of reflecting boundaries in the intermediate structural stage of the Chu Sarysuyskiy depression based on results of vertical seismic profilling

    Energy Technology Data Exchange (ETDEWEB)

    Davydov, N.G.; Kiselevskiy, Yu.N.

    1983-01-01

    A computer (EVM) and an ASOI-VSP-SK program complex are used to analyze data from seismic exploration and acoustical logging with interval by interval calculation of the velocity every four meters. Vertical seismic profilling (VSP) results are used to identify all the upper layers as reference layers. The basic reference level, the third, which corresponds to the floor of the carbonate middle to upper Visean series, is not sustained due to the thin layered state of the terrigeneous section. Based on data from vertical seismic profilling, the reflected wave method (MOV) and the common depth point method (MOGT), the reference 3-a and 6-a levels are identified. Deep reflections of the seventh, 7-a and Rf, approximately confined to the roof and floor of the lower Paleozoic deposits and the upper part of the upper reef series, are noted in the series of the Caledonian cap of the Prebaykal massifs based on vertical seismic profilling. Collector levels are noted on the basis of the frequency of the wave spectra and from the absorption coefficient in the Testas structure and in other low amplitude structures. The insufficiency of the depth capability of the common depth point method and the poor knowledge level of seismic exploration of the section of the lower Paleozoa and the upper Proterozoa of the Chu Sarysuyskiy depresion are noted.

  10. Transmission of the agent of sheep scrapie to deer results in PrPSc with two distinct molecular profiles

    Science.gov (United States)

    The purpose of this work was to determine susceptibility of white-tailed deer (WTD) to the agent of sheep scrapie and to compare the resultant PrPSc to that of the original inoculum and chronic wasting disease (CWD). We inoculated WTD by a natural route of exposure (concurrent oral and intranasal (I...

  11. Show-Bix &

    DEFF Research Database (Denmark)

    2014-01-01

    The anti-reenactment 'Show-Bix &' consists of 5 dias projectors, a dial phone, quintophonic sound, and interactive elements. A responsive interface will enable the Dias projectors to show copies of original dias slides from the Show-Bix piece ”March på Stedet”, 265 images in total. The copies...... are made from digital scans of the original dias slides located in the collection of the Museum of Contemporary Art in Roskilde. In front of the audience entering the space and placed on it’s own stand, is an original 60s style telephone with turning dial. Action begins when the audience lift the phone...... and dial a number. Any number will make the Dias change. All numbers are also assigned to specific sound documents: clips form rare interviews and the complete sound-re-enactment of the Show-Bix piece ‘Omringning’ (‘Surrounding’) in five channels (a quintophonie). This was originally produced...

  12. Show and Tell

    DEFF Research Database (Denmark)

    2013-01-01

    Fredag d. 1 november blev Kunsthal Charlottenborg indtaget af performanceprogrammet Show & Tell med et bredspektret program af danske og internationale kunstnere indenfor performance-, lyd- og installationskunst. Programmet præsenterer værker, der undersøger kroppens stadig mere symbiotiske forhold...... og studienævnet på Performance-design. Show & Tell - Performance program: kl. 16.30-19 Adresse: Kunsthal Charlottenborg, Nyhavn 2, 1051 København K...

  13. [Profile of sexual behavior in 12- to 19-year-old Mexican adolescents. Results of ENSA 2000].

    Science.gov (United States)

    González-Garza, Carlos; Rojas-Martínez, Rosalba; Hernández-Serrato, María I; Olaiz-Fernández, Gustavo

    2005-01-01

    To describe traits associated with sexual behaviors in Mexican adolescents, their knowledge about contraception, and factors associated with pregnancy and utilization of contraceptives during first sexual intercourse. Data from the Mexican National Health Survey 2000 (ENSA 2000) were analyzed. This study, conducted between September 1999 and March 2000, was a complex survey with a probabilistic, stratified, and cluster sampling design. The sample population included 15 241 adolescents 12 to 19 years of age. Data analysis included descriptive statistics and a chi-squared test for differences of proportions; also, logistic regression was used to obtain odds ratios. A total of 69.2% adolescents reported knowledge of at least one contraception method; 16.4% of subjects reported having had sexual intercourse. Males initiated sexual intercourse earlier than females and only 37% of all adolescents utilized contraceptives during their first sexual intercourse. Logistic regression analysis showed that being male, having a higher education, having knowledge of at least one contraception method, and having initiated sexual intercourse at an older age, were factors associated with utilization of a contraception method in their first sexual intercourse. A total of 55.7% of sexually active female adolescents had been pregnant. Pregnancy among adolescents was associated with low educational level, sexual activity at early age, and to have sometime lived in free union. The majority of sexually active adolescents did not use contraception during their first sexual intercourse, rendering them at risk of unwanted pregnancies.

  14. Contig sequences and their annotation (amino acid sequence and results of homology search), and expression profile - Dicty_cDB | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available List Contact us Dicty_cDB Contig sequences and their annotation (amino acid sequence and results of homology... search), and expression profile Data detail Data name Contig sequences and their annotation (amino acid seq...f data contents Contig sequences of cDNA sequences of Dictyostelium discoideum and the...EST-3'EST-ligated sequence and full-length cDNA sequence by the assembly program ...Phrap ( http://www.phrap.org/index.html ). Link to the list of clones constituting the contig, the information on its mapping to the

  15. Code for the calculation of the instrumental profile: preliminary results. (Spanish Title: Código para el cálculo del perfil instrumental: resultados preliminares)

    Science.gov (United States)

    Pintado, O. I.; Santillán, L.; Marquetti, M. E.

    All images obtained with a telescope are distorted by the instrument. This distorsion is known as instrumental profile or instrumental broadening. The deformations in the spectra could introduce large errors in the determination of different parameters, especially in those dependent on the spectral lines shapes, such as chemical abundances, winds, microturbulence, etc. To correct this distortion, in some cases, the spectral lines are convolved with a Gaussian function and in others the lines are widened with a fixed value. Some codes used to calculate synthetic spectra, as SYNTHE, include this corrections. We present results obtained for the spectrograph REOSC and EBASIM of CASLEO.

  16. Acute Myocardial Infarction and Pulmonary Diseases Result in Two Different Degradation Profiles of Elastin as Quantified by Two Novel ELISAs

    DEFF Research Database (Denmark)

    Skjøt-Arkil, Helene; Clausen, Rikke E; Rasmussen, Lars M

    2013-01-01

    and ELM-2 ELISAs was evaluated in patients with acute myocardial infarction (AMI), no AMI, high coronary calcium, or low coronary calcium. The serological release of ELM-2 in patients with chronic obstructive pulmonary disease (COPD) or idiopathic pulmonary fibrosis (IPF) was compared to controls. RESULTS......BACKGROUND: Elastin is a signature protein of the arteries and lungs, thus it was hypothesized that elastin is subject to enzymatic degradation during cardiovascular and pulmonary diseases. The aim was to investigate if different fragments of the same protein entail different information associated...... and no correlation was observed between ELM-2 and ELM. ELM-2 was not elevated in the COPD and IPF patients and was not correlated to ELM. ELM was shown to be correlated with smoking habits (ppulmonary diseases...

  17. Combination of niacin extended-release and simvastatin results in a less atherogenic lipid profile than atorvastatin monotherapy

    Directory of Open Access Journals (Sweden)

    William Insull Jr

    2010-11-01

    Full Text Available William Insull Jr1, Peter P Toth2, H Robert Superko3, Roopal B Thakkar4, Scott Krause4, Ping Jiang4, Rhea A Parreno4, Robert J Padley41Baylor College of Medicine and Methodist Hospital, Houston, Texas; 2University of Illinois College of Medicine, Peoria, Illinois; 3Celera, Alameda, California, Mercer University, Atlanta, Georgia; 4Abbott, Abbott Park, Illinois, USAObjective: To compare the effects of combination niacin extended-release + simvastatin (NER/S versus atorvastatin alone on apolipoproteins and lipid fractions in a post hoc analysis from SUPREME, a study which compared the lipid effects of niacin extended-release + simvastatin and atorvastatin in patients with hyperlipidemia or mixed dyslipidemia.Patients and methods: Patients (n = 137 with dyslipidemia (not previously receiving statin therapy or having discontinued any lipid-altering treatment 4–5 weeks prior to the study received NER/S (1000/40 mg/day for four weeks, then 2000/40 mg/day for eight weeks or atorvastatin 40 mg/day for 12 weeks. Median percent changes in apolipoprotein (apo A-1, apo B, and the apo B:A-I ratio, and nuclear magnetic resonance lipoprotein subclasses from baseline to week 12 were compared using the Wilcoxon rank-sum test and Fisher’s exact test.Results: NER/S treatment produced significantly greater percent changes in apo A-I and apo B:A-I, and, at the final visit, apo B <80 mg/dL was attained by 59% versus 33% of patients, compared with atorvastatin treatment (P = 0.003. NER/S treatment resulted in greater percent reductions in calculated particle numbers for low-density lipoprotein (LDL, 52% versus 43%; P = 0.022, small LDL (55% versus 45%; P = 0.011, very low-density lipoprotein (VLDL and total chylomicrons (63% versus 39%; P < 0.001, and greater increases in particle size for LDL (2.7% versus 1.0%; P = 0.007 and VLDL (9.3% versus 0.1%; P < 0.001, compared with atorvastatin.Conclusion: NER/S treatment significantly improved apo A-I levels and the apo

  18. Performance profile of a FDG-PET cancer screening program for detecting gastric cancer: results from a nationwide Japanese survey.

    Science.gov (United States)

    Minamimoto, Ryogo; Senda, Michio; Jinnouchi, Seishi; Terauchi, Takashi; Yoshida, Tsuyoshi; Inoue, Tomio

    2014-05-01

    It has been reported that gastric cancer is the sixth most common cancer found during the (18)F-fluorodeoxyglucose-positron emission tomography (FDG-PET) cancer screening program, which is defined as cancer screening of asymptomatic subjects using FDG-PET(/CT) (in combination with other screening tests or not). The aim of this study was to analyze the detection rate and the effectiveness of the FDG-PET cancer screening program at detecting gastric cancer between 2006 and 2009 in Japan. A total of 153,775 asymptomatic subjects (92,255 men, 61,520 women) between 30 and 80 years old underwent the FDG-PET cancer screening program. Of these, we analyzed 790 cases with findings of possible gastric cancer in any screening test. The number of cases who were verified to have gastric cancer was 124. Among these, only 47 cases were detected by FDG-PET, which resulted in a relative sensitivity of 37.9% and a positive predictive value of 33.6%. The relative sensitivity of FDG-PET was much lower than those of gastric endoscopy and the serum pepsinogen test. The FDG-PET screening program in Japan detected some cases of early-stage gastric cancer, but this was not achieved using FDG-PET alone but in combination with gastric endoscopy. Gastric endoscopy should be included in FDG-PET cancer screening programs to screen for gastric cancer.

  19. The safety profile of perflutren microsphere contrast echocardiography during rest and stress imaging: results from an Australian multicentre cohort.

    Science.gov (United States)

    Platts, David G; Luis, Sushil A; Roper, Damian; Burstow, Darryl; Call, Tony; Forshaw, Anthony; Pascoe, Roess

    2013-12-01

    Contrast enhanced echocardiography (CEE) is utilised when sub-optimal image quality results in non-diagnostic echocardiograms. However, there have been numerous safety notices issued by regulatory authorities regarding rare but potentially serious adverse reactions (AR). This multi-centre, retrospective analysis was performed to assess the short-term safety of CEE in a broad range of indications. All CEE performed over 58 months at three institutions were assessed for AR within 30 min. A total of 5956 CEE were performed in 5576 patients. A total of 4903 were stress CEE and 1053 resting CCE. Bolus administration in 5719, infusion in 237 cases; 89.9% of CCE were outpatients. Commonest CEE indication was functional stress testing (82.3%). There were 16 AR related to CEE (0.27%). All AR were mild, transient and all patients made a full recovery. No cases of serious anaphylaxis or death within 30 min of contrast administration. Comparing those with and without an AR, there were no significant differences in age, gender, BMI, LVEF, patient location, exam type or RVSP. There was a slightly increased likelihood of an AR during infusion versus bolus dosing (p = 0.02). CEE is a safe investigation in a broad range of indications and clinical scenarios. AR are very rare, mild and transient. Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.

  20. Metabolic analysis revealed altered amino acid profiles in Lupinus albus organs as a result of boron deficiency.

    Science.gov (United States)

    Alves, Marta; Chicau, Paula; Matias, Helena; Passarinho, José; Pinheiro, Carla; Ricardo, Cândido Pinto

    2011-07-01

    We analysed the changes in the metabolites of Lupinus albus organs (leaf-blades, petioles, apexes, hypocotyls and roots) as a consequence of B deficiency. The deficiency did not affect malate concentration and induced only minor changes in the sugar content, suggesting that the carbohydrate metabolism is little affected by the deficiency. Contrarily, marked changes in the content of free amino acids were observed, with some specific variations associated with the different organs. These changes indicate that various aspects of metabolism implicated in the amino acid accumulation were affected by B deficiency. Most of the detected changes appear to have implications with some stress responses or signalling processes. Asparagine and proline that increase in many stresses also accumulated in petioles, apexes and hypocotyls. Accumulation of γ-aminobutyric acid shunt amino acids, indicative of production of reactive oxygen species, occurs in the same three organs and also the roots. The increase in the branched-chain amino acids, observed in all organs, suggests the involvement of B with the cytoskeleton, whereas glycine decrease in leaf-blades and active growing organs (apexes and roots) could be associated with the proposed role of this amino acids in plant signalling in processes that might be associated with the decreased growth rates observed in B deficiency. Despite the admitted importance of free amino acids in plant metabolism, the available information on this matter is scarce. So our results bring new information concerning the effects of B deficiency in the metabolism of the several L. albus organs. Copyright © Physiologia Plantarum 2011.

  1. Cigarette smoking and schizophrenia: a specific clinical and therapeutic profile? Results from the FACE-Schizophrenia cohort.

    Science.gov (United States)

    Mallet, J; Le Strat, Y; Schürhoff, F; Mazer, N; Portalier, C; Andrianarisoa, M; Aouizerate, B; Berna, F; Brunel, L; Capdevielle, D; Chereau, I; D'Amato, T; Denizot, H; Dubreucq, J; Faget, C; Gabayet, F; Lançon, C; Llorca, P M; Misdrahi, D; Rey, R; Roux, P; Schandrin, A; Urbach, M; Vidailhet, P; Fond, G; Dubertret, C

    2017-10-03

    Tobacco use is common in patients with schizophrenia (SZ) but little is known on the role of tobacco in the physiopathology or on the course of the disease. Only few studies embrace an extensive examination of clinical and therapeutic characteristics in stabilized patients. The objective of the present study was to determine the prevalence of tobacco smoking in stabilized SZ outpatients and the clinical and treatment characteristics associated with daily tobacco use in a large community-dwelling sample of patients. Three-hundred-and-sixty-one patients were included in the network of the FondaMental Expert Centers for Schizophrenia. Current tobacco status was self-declared. 53.7% were smokers. Mean age at tobacco onset was 17.2years old. In multivariate analyses, after adjustment for confounding factors, positive symptoms and mean daily antipsychotic dose were associated with a higher frequency of tobacco use (OR=1.06 95%IC[1.02-1.12], for positive symptoms, OR=1.1, 95%IC[1.02-1.18] for daily antipsychotic dose). Education level, negative symptoms, anticholinergic agents, clozapine or aripiprazole administration were independently associated with a lower frequency of tobacco use (respectively OR=0.87, 95%IC [0.79, 0.95], OR=0.95, 95%IC[0.91-0.98], OR=0.41, 95%IC[0.22-0.76], OR=0.56, 95%IC=[0.32, 0.99] and OR=0.49, 95%IC [0.26-0.91]). The prevalence of current tobacco smoking in a French community-dwelling SZ patients is higher that observed in the general population. Patients with tobacco use present clinical and therapeutic specificities that may involve interaction between cholinergic-nicotinic and dopaminergic systems. The present study suggests that some therapeutics may improve daily smoking behavior in smokers. These results should be confirmed in longitudinal studies. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. A Mediterranean-type diet is associated with better metabolic profile in urban Polish adults: Results from the HAPIEE study

    Science.gov (United States)

    Grosso, Giuseppe; Stepaniak, Urszula; Micek, Agnieszka; Topor-Mądry, Roman; Stefler, Denes; Szafraniec, Krystyna; Bobak, Martin; Pająk, Andrzej

    2015-01-01

    Objective The aim of this study was to evaluate the relationship between adherence to a Mediterranean-type diet and metabolic syndrome (MetS) in the Polish arm of the Health, Alcohol and Psychosocial factors In Eastern Europe (HAPIEE) cohort study. Materials/methods A cross-sectional survey including 8821 adults was conducted in Krakow, Poland. Food intake was evaluated through a validated food frequency questionnaire and adherence to the dietary pattern was assessed using a score specifically developed for non-Mediterranean countries (MedTypeDiet score). Linear and logistic regression models were performed to estimate beta and odds ratios (ORs) and 95% confidence intervals (CIs), respectively. Results Significant associations between the MedTypeDiet score and waist circumference (β = − 0.307 ± 0.239 cm), systolic blood pressure (β = − 0.440 ± 0.428 mmHg), and triglycerides (β = − 0.021 ± 0.016 mmol/L) were observed. After multivariable adjustment, individuals in the highest quartile of the score were less likely to have MetS, central obesity, high triglycerides, and hypertension. Increase of one standard deviation of the score was associated with 7% less odds of having MetS (OR 0.93, 95% CI: 0.88, 0.97). When analyzing the relation of single components of the MedTypeDiet score, wine, dairy products, and the total unsaturated:saturated fatty acids ratio were associated with MetS. Conclusions Adherence to a Mediterranean-like diet may decrease the risk of MetS also among non-Mediterranean populations. PMID:25752843

  3. Superposition of nanostructures on microrough titanium–aluminum–vanadium alloy surfaces results in an altered integrin expression profile in osteoblasts

    Science.gov (United States)

    Gittens, Rolando A.; Olivares-Navarrete, Rene; Hyzy, Sharon L.; Sandhage, Kenneth H.; Schwartz, Zvi; Boyan, Barbara D.

    2014-01-01

    Recent studies of new surface modifications that superimpose well-defined nanostructures on microrough implants, thereby mimicking the hierarchical complexity of native bone, report synergistically enhanced osteoblast maturation and local factor production at the protein level compared to growth on surfaces that are smooth, nanorough, or microrough. Whether the complex micro/nanorough surfaces enhance the osteogenic response by triggering similar patterns of integrin receptors and their associated signaling pathways as with well-established microrough surfaces, is not well understood. Human osteoblasts (hOBs) were cultured until confluent for gene expression studies on tissue culture polystyrene (TCPS) or on titanium alloy (Ti6Al4V) disks with different surface topographies: smooth, nanorough, microrough, and micro/nanorough surfaces. mRNA expression of osteogenesis-related markers such as osteocalcin (BGLAP) and bone sialoprotein (BSP), bone morphogenetic protein 2 (BMP2), BMP4, noggin (NOG) and gremlin 1 (GREM1) were all higher on microrough and micro/nanorough surfaces, with few differences between them, compared to smooth and nanorough groups. Interestingly, expression of integrins α1 and β2, which interact primarily with collagens and laminin and have been commonly associated with osteoblast differentiation on microrough Ti and Ti6Al4V, were expressed at lower levels on micro/nanorough surfaces compared to microrough ones. Conversely, the av subunit, which binds ligands such as vitronectin, osteopontin, and bone sialoprotein among others, had higher expression on micro/nanorough surfaces concomitantly with regulation of the β3 mRNA levels on nanomodified surfaces. These results suggest that the maturation of osteoblasts on micro/nanorough surfaces may be occurring through different integrin engagement than those established for microrough-only surfaces. PMID:25158204

  4. ANALYSIS OF THE RESULTS OF PRESBYOPIA CORRECTION WITH APPLICATION OF A BI-ASPHERIC MULTIFOCAL PROFILE ON THE CORNEA BY THE PRK FOR PATIENTS WITH MYOPIA AND HYPEROPIA

    Directory of Open Access Journals (Sweden)

    E. N. Eskina

    2017-01-01

    Full Text Available Purpose. To compare the efficacy, safety and predictability of presbyopia correction by the PRK with application of a bi-aspheric multifocal profile on the cornea with PresbyMax software for patients with hyperopia and myopia.Patients and methods: There were 2 patients group of 25 people each (50 eyes. They were operated with PRK method with application of a multifocal biaspheric profile for simultaneous correction of ametropia and presbyopia. Group I — patients with myopia and presbyopia. Group II — patients with hypermetropia and presbyopia.Results. In the group 1 year post operation binocular DUCVA was 0,93±0,19, NUCVA — 0,86±0,14, IUCVA — 0,67±0,11. Only two eyes (4 % lost DBCVA on the 1–2 lines. Target refraction of a dominant eye — emmetropia was 72 % of patients, 28 % observed myopia of –0,75 D. Target refraction of a nondominant eye was –0,75, 68 % had this result, 28 % had deviation –0,50 D of the target refraction, and 4 % — 1,00 D. In group 2 a year post operation binocular DUCVA was 0,96±0,16, NUCVA — 0,77±0,19, IUCVA — 0,64±0,15. Only two eyes (4 % lost DBCVA on the 1–2 lines. Target refraction of a dominant eye — emmetropia was 72 % of patients, 28 % observed myopia of –0,75 D. Target refraction of a nondominant eye was –0,75, 68 % had this result, 32 % had deviation to –0,75 D of the target refraction.Conclusions. PRK with application of a bi-aspheric multifocal profile on the cornea using PresbyMAX software and µ-monovision is effective for correction myopia and hypermetropia. Simultaneous correction of ametropia and presbyopia by the PRK method with the application of the multifocal biospheric profile to the cornea with the formation of a slight anisometropia (the concept of µ-monovision with the PresbyMax software is an effective, safe and predictable method of surgical intervention, both in myopia and hypermetropia.

  5. Talking with TV shows

    DEFF Research Database (Denmark)

    Sandvik, Kjetil; Laursen, Ditte

    2014-01-01

    User interaction with radio and television programmes is not a new thing. However, with new cross-media production concepts such as X Factor and Voice, this is changing dramatically. The second-screen logic of these productions encourages viewers, along with TV’s traditional one-way communication...... mode, to communicate on interactive (dialogue-enabling) devices such as laptops, smartphones and tablets. Using the TV show Voice as our example, this article shows how the technological and situational set-up of the production invites viewers to engage in new ways of interaction and communication....... More specifically, the article demonstrates how online comments posted on the day of Voice’s 2012 season finale can be grouped into four basic action types: (1) Invitation to consume content, (2) Request for participation, (3) Request for collaboration and (4) Online commenting. These action types...

  6. Um show de cacau

    OpenAIRE

    Rezende, José Francisco; UNIGRANRIO / PPGA; Mello, Simone; UNIGRANRIO

    2016-01-01

    O caso de ensino apresenta a trajetória de Alexandre Tadeu da Costa e da chocolateria Cacau Show. Seu objetivo é levar os estudantes a identificar alternativas e tomar decisões sobre posicionamento para continuidade do desenvolvimento de vantagens competitivas, sustentação de competência logística e possíveis abordagens ao mercado externo. 

  7. Profiling cancer

    DEFF Research Database (Denmark)

    Ciro, Marco; Bracken, Adrian P; Helin, Kristian

    2003-01-01

    In the past couple of years, several very exciting studies have demonstrated the enormous power of gene-expression profiling for cancer classification and prediction of patient survival. In addition to promising a more accurate classification of cancer and therefore better treatment of patients......, gene-expression profiling can result in the identification of novel potential targets for cancer therapy and a better understanding of the molecular mechanisms leading to cancer....

  8. Profiles of organic food consumers in a large sample of French adults: results from the Nutrinet-Santé cohort study.

    Science.gov (United States)

    Kesse-Guyot, Emmanuelle; Péneau, Sandrine; Méjean, Caroline; Szabo de Edelenyi, Fabien; Galan, Pilar; Hercberg, Serge; Lairon, Denis

    2013-01-01

    Lifestyle, dietary patterns and nutritional status of organic food consumers have rarely been described, while interest for a sustainable diet is markedly increasing. Consumer attitude and frequency of use of 18 organic products were assessed in 54,311 adult participants in the Nutrinet-Santé cohort. Cluster analysis was performed to identify behaviors associated with organic product consumption. Socio-demographic characteristics, food consumption and nutrient intake across clusters are provided. Cross-sectional association with overweight/obesity was estimated using polytomous logistic regression. Five clusters were identified: 3 clusters of non-consumers whose reasons differed, occasional (OCOP, 51%) and regular (RCOP, 14%) organic product consumers. RCOP were more highly educated and physically active than other clusters. They also exhibited dietary patterns that included more plant foods and less sweet and alcoholic beverages, processed meat or milk. Their nutrient intake profiles (fatty acids, most minerals and vitamins, fibers) were healthier and they more closely adhered to dietary guidelines. In multivariate models (after accounting for confounders, including level of adherence to nutritional guidelines), compared to those not interested in organic products, RCOP participants showed a markedly lower probability of overweight (excluding obesity) (25 ≤ body mass indexorganic products, a sizeable group in our sample, exhibit specific socio-demographic characteristics, and an overall healthy profile which should be accounted for in further studies analyzing organic food intake and health markers.

  9. Taking in a Show.

    Science.gov (United States)

    Boden, Timothy W

    2016-01-01

    Many medical practices have cut back on education and staff development expenses, especially those costs associated with conventions and conferences. But there are hard-to-value returns on your investment in these live events--beyond the obvious benefits of acquired knowledge and skills. Major vendors still exhibit their services and wares at many events, and the exhibit hall is a treasure-house of information and resources for the savvy physician or administrator. Make and stick to a purposeful plan to exploit the trade show. You can compare products, gain new insights and ideas, and even negotiate better deals with representatives anxious to realize returns on their exhibition investments.

  10. Routine use of microarray-based gene expression profiling to identify patients with low cytogenetic risk acute myeloid leukemia: accurate results can be obtained even with suboptimal samples

    Directory of Open Access Journals (Sweden)

    de la Blétière Diane Raingeard

    2012-01-01

    Full Text Available Abstract Background Gene expression profiling has shown its ability to identify with high accuracy low cytogenetic risk acute myeloid leukemia such as acute promyelocytic leukemia and leukemias with t(8;21 or inv(16. The aim of this gene expression profiling study was to evaluate to what extent suboptimal samples with low leukemic blast load (range, 2-59% and/or poor quality control criteria could also be correctly identified. Methods Specific signatures were first defined so that all 71 acute promyelocytic leukemia, leukemia with t(8;21 or inv(16-AML as well as cytogenetically normal acute myeloid leukemia samples with at least 60% blasts and good quality control criteria were correctly classified (training set. The classifiers were then evaluated for their ability to assign to the expected class 111 samples considered as suboptimal because of a low leukemic blast load (n = 101 and/or poor quality control criteria (n = 10 (test set. Results With 10-marker classifiers, all training set samples as well as 97 of the 101 test samples with a low blast load, and all 10 samples with poor quality control criteria were correctly classified. Regarding test set samples, the overall error rate of the class prediction was below 4 percent, even though the leukemic blast load was as low as 2%. Sensitivity, specificity, negative and positive predictive values of the class assignments ranged from 91% to 100%. Of note, for acute promyelocytic leukemia and leukemias with t(8;21 or inv(16, the confidence level of the class assignment was influenced by the leukemic blast load. Conclusion Gene expression profiling and a supervised method requiring 10-marker classifiers enable the identification of favorable cytogenetic risk acute myeloid leukemia even when samples contain low leukemic blast loads or display poor quality control criterion.

  11. Deep structure and historical earthquakes in the Calabrian subduction zone (Southern Italy): preliminary results from multi-channel seismic reflection profiles

    Science.gov (United States)

    Gallais, F.; Gutscher, M.; Torelli, L.; Polonia, A.; Riminucci, F.

    2009-12-01

    The Calabrian subduction zone is located in the complex Central Mediterranean area. This subduction is characterized by the presence of deep earthquakes under the Tyrrhenian Sea down to 500 km depth. The Tethyan remnant Ionian slab descends towards the NW at a dip of about 70° and is associated with an active volcanic arc (the Aeolian Islands). Recently reported GPS and seismicity studies suggest that the subduction of the Ionian lithosphere beneath the Calabrian Arc may be locally still active, though at very slow rates (thrust earthquakes, characteristic of active subduction zone, suggests that if subduction is active, the fault plane may be locked since the instrumental period. To seek evidence of continuous tectonic activity of the Calabrian system, we present preliminary results from reprocessed 96-channels seismic reflection profiles (French Archimede cruise, 1997) offshore Sicily. This analysis permits to recognize a well-defined stratigraphy in the Ionian Abyssal Plain, this stratigraphy becomes difficult to follow under the deformed Calabrian Prism. But the joint interpretation with the reprocessed PM01 profile (French PRISMED cruise, 1994) helps constrain this interpretation and to image some characteristic structures of an accretionary wedge (fore/back-thrusts, basal decollement...). This study also include interpretation of a more recent Italian seismic cruise (Calamare, 2008) and CROP profiles. This work will help to prepare a future cruise proposal (CIRCEE, to be submitted in January 2010) to study the Calabrian subduction with OBS, MCS seismic, heat-flow measurements and sediment coring. The goals are : 1/ to image the deep structure of this subduction zone, 2/ to characterize its thermal state to deduce a geometry of the seismogenic part of the plate interface and add new constraints on seismic risk linked with the Calabrian subduction.

  12. Dietary fiber showed no preventive effect against colon and rectal cancers in Japanese with low fat intake: an analysis from the results of nutrition surveys from 23 Japanese prefectures

    Directory of Open Access Journals (Sweden)

    Sugawara Kazuo

    2001-10-01

    Full Text Available Abstract Background Since Fuchs' report in 1999, the reported protective effect of dietary fiber from colorectal carcinogenesis has led many researchers to question its real benefit. The aim of this study is to evaluate the association between diet, especially dietary fiber and fat and colorectal cancer in Japan. Methods A multiple regression analysis (using the stepwise variable selection method was performed using the standardized mortality ratios (SMRs of colon and rectal cancer in 23 Japanese prefectures as objective variables and dietary fiber, nutrients and food groups as explanatory variables. Results As for colon cancer, the standardized partial correlation coefficients were positively significant for fat (1,13, P = 0.000, seaweeds (0.41, P = 0.026 and beans (0.45, P = 0.017 and were negatively significant for vitamin A (-0.63, P = 0.003, vitamin C (-0.42, P = 0.019 and yellow-green vegetables (-0.37, P = 0.046. For rectal cancer, the standardized partial correlation coefficient in fat (0.60, P = 0.002 was positively significant. Dietary fiber was not found to have a significant relationship with either colon or rectal cancers. Conclusions This study failed to show any protective effect of dietary fiber in subjects with a low fat intake (Japanese in this analysis, which supports Fuchs' findings in subjects with a high fat intake (US Americans.

  13. STRESSORS, SYMPTOM PROFILE, AND PREDICTORS OF ADJUSTMENT DISORDER IN CANCER PATIENTS. RESULTS FROM AN EPIDEMIOLOGICAL STUDY WITH THE COMPOSITE INTERNATIONAL DIAGNOSTIC INTERVIEW, ADAPTATION FOR ONCOLOGY (CIDI-O).

    Science.gov (United States)

    Hund, Bianca; Reuter, Katrin; Härter, Martin; Brähler, Elmar; Faller, Hermann; Keller, Monika; Schulz, Holger; Wegscheider, Karl; Weis, Joachim; Wittchen, Hans-Ulrich; Koch, Uwe; Friedrich, Michael; Mehnert, Anja

    2016-02-01

    We aimed to investigate type and frequency of stressors, predominant symptom profiles, and predictors of adjustment disorders (AD) in cancer patients across major tumor entities. In this epidemiological study, we examined 2,141 cancer patients out of 4,020 screened with the Composite International Diagnostic Interview, adaptation for oncology (CIDI-O). AD were operationalized as subthreshold disorders according to DSM-IV criteria. In our sample, 265 out of 2,141 patients (12.4%) met all criteria for AD (unweighted 4-week prevalence). The disclosure of the cancer diagnosis, relapse or metastases, and cancer treatments were most frequently described as stressors associated with depressive or anxious symptoms. With regard to AD symptom profiles, patients showed high prevalence rates of affective symptoms according to the DSM-IV criteria of Major Depression: The highest prevalence rates were found for cognitive disturbances (concentration and memory problems) (88%), sleeping disturbances (86%), and depressive mood (83%). We found sex, education, and metastasis as significant predictors for AD. Higher education was the most influential predictor. Men were half as likely to report symptoms fulfilling the AD criteria as women. Patients with metastasized tumors had a more than 80% higher risk of AD than those without metastasis. However, the explained variance of our model is very small (Nagelkerke's R² = 0.08). Patients with AD can be identified using a standardized instrument and deserve clinical attention, as they often show severe clinical symptoms and impairments. Improving the clinical conceptualization of AD by the adding-on of potential stress-response-symptoms is necessary to identify severe psychological strain. © 2015 Wiley Periodicals, Inc.

  14. [Systemic Antimicrobials Consumption and Expenditures in Departments of Surgery of Multi-Profile Hospitals in the Russian Federation and the Republic of Belarus: Results of Multicentre Pharmacoepidemiological Study].

    Science.gov (United States)

    Belkova, Yu A; Rachina, S A; Kozlov, R S; Mishchenko, V M; Pavlukov, R A; Abubakirova, A I; Berezhanskiy, B V; Eliseeva, E V; Zubareva, N A; Karpov, I A; Kopylova, I A; Palyutin, Sh Kh; Portnyagina, U S; Pribytkova, O V; Samuylo, E K

    2016-01-01

    The results of the systemic antimicrobials (AM) consumption and expenditures assessment in the departments of surgery of multi-profile hospitals in different regions of the Russian Federation and the Republic of Belarus in 2009-2010 based on retrospective collection and analysis of the data from the hospital expenditure notes using ATC/DDD methodology are presented. The average AM consumption and expenditure rates in the above mentioned departments varied from 24.9 DDD/100 bed-days to 61.7 DDD/100 bed-days depending on the department profile, with beta-lactams (cephalosporins and penicillins) share in the consumption being as high as 70-90%, followed by fluoroquinolones and aminoglycosides. Only 55-70% of the consumed AM belonged to the drugs of choice, whereas the improper AM consumption and expenditure rates amounted up to 10-18%. The study outputs can be used for the budget allocation and AM distribution improvement in the departments of surgery, as well as for the development and efficacy control of the local antimicrobial stewardship programs.

  15. Liquid drops on a surface: using density functional theory to calculate the binding potential and drop profiles and comparing with results from mesoscopic modelling.

    Science.gov (United States)

    Hughes, Adam P; Thiele, Uwe; Archer, Andrew J

    2015-02-21

    The contribution to the free energy for a film of liquid of thickness h on a solid surface due to the interactions between the solid-liquid and liquid-gas interfaces is given by the binding potential, g(h). The precise form of g(h) determines whether or not the liquid wets the surface. Note that differentiating g(h) gives the Derjaguin or disjoining pressure. We develop a microscopic density functional theory (DFT) based method for calculating g(h), allowing us to relate the form of g(h) to the nature of the molecular interactions in the system. We present results based on using a simple lattice gas model, to demonstrate the procedure. In order to describe the static and dynamic behaviour of non-uniform liquid films and drops on surfaces, a mesoscopic free energy based on g(h) is often used. We calculate such equilibrium film height profiles and also directly calculate using DFT the corresponding density profiles for liquid drops on surfaces. Comparing quantities such as the contact angle and also the shape of the drops, we find good agreement between the two methods. We also study in detail the effect on g(h) of truncating the range of the dispersion forces, both those between the fluid molecules and those between the fluid and wall. We find that truncating can have a significant effect on g(h) and the associated wetting behaviour of the fluid.

  16. The presence of resilience is associated with a healthier psychological profile in intensive care unit (ICU) nurses: results of a national survey.

    Science.gov (United States)

    Mealer, Meredith; Jones, Jacqueline; Newman, Julia; McFann, Kim K; Rothbaum, Barbara; Moss, Marc

    2012-03-01

    ICU nurses are repeatedly exposed to work related stresses resulting in the development of psychological disorders including posttraumatic stress disorder and burnout syndrome. Resilience is a learnable multidimensional characteristic enabling one to thrive in the face of adversity. In a national survey, we sought to determine whether resilience was associated with healthier psychological profiles in intensive care unit nurses. Surveys were mailed to 3500 randomly selected ICU nurses across the United States and included: demographic questions, the Posttraumatic Diagnostic Scale, Hospital Anxiety and Depression Scale, Maslach Burnout Inventory and the Connor-Davidson Resilience Scale. Overall, 1239 of the mailed surveys were returned for a response rate of 35%, and complete data was available on a total of 744 nurses. Twenty-two percent of the intensive care unit nurses were categorized as being highly resilient. The presence of high resilience in these nurses was significantly associated with a lower prevalence of posttraumatic stress disorder, symptoms of anxiety or depression, and burnout syndrome (burnout syndrome (pburnout syndrome in intensive care unit nurses. Future research is needed to better understand coping mechanisms employed by highly resilient nurses and how they maintain a healthier psychological profile. Copyright © 2011 Elsevier Ltd. All rights reserved.

  17. Metabolite Profile Resulting from the Activation/Inactivation of 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 2-Methyltetrahydro-β-carboline by Oxidative Enzymes

    Directory of Open Access Journals (Sweden)

    Tomás Herraiz

    2013-01-01

    Full Text Available Metabolic enzymes are involved in the activation/deactivation of the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyiridine (MPTP neurotoxin and its naturally occurring analogs 2-methyltetrahydro-β-carbolines. The metabolic profile and biotransformation of these protoxins by three enzymes, monoamine oxidase (MAO, cytochrome P450, and heme peroxidases (myeloperoxidase and lactoperoxidase, were investigated and compared. The metabolite profile differed among the enzymes investigated. MAO and heme peroxidases activated these substances to toxic pyridinium and β-carbolinium species. MAO catalyzed the oxidation of MPTP to 1-methyl-4-phenyl-2,3-dihydropyridinium cation (MPDP+, whereas heme peroxidases catalyzed the oxidation of MPDP+ to 1-methyl-4-phenylpyridinium (MPP+ and of 2-methyltetrahydro-β-carboline to 2-methyl-3,4-dihydro-β-carbolinium cation (2-Me-3,4-DHβC+. These substances were inactivated by cytochrome P450 2D6 through N-demethylation and aromatic hydroxylation (MPTP and aromatic hydroxylation (2-methyltetrahydro-β-carboline. In conclusion, the toxicological effects of these protoxins might result from a balance between the rate of their activation to toxic products (i.e., N-methylpyridinium-MPP+ and MPDP+- and N-methyl-β-carbolinium—βC+— by MAO and heme peroxidases and the rate of inactivation (i.e., N-demethylation, aromatic hydroxylation by cytochrome P450 2D6.

  18. KSHV oral shedding and plasma viremia result in significant changes in the extracellular tumorigenic miRNA expression profile in individuals infected with the malaria parasite.

    Science.gov (United States)

    Ikoma, Minako; Gantt, Soren; Casper, Corey; Ogata, Yuko; Zhang, Qing; Basom, Ryan; Dyen, Michael R; Rose, Timothy M; Barcy, Serge

    2018-01-01

    Kaposi's sarcoma herpesvirus (KSHV) is the etiological agent of Kaposi's sarcoma (KS). Both KSHV and HIV infections are endemic in Uganda, where KS is among the most common cancers in HIV-infected individuals. Recent studies examined the use of small RNAs as biomarkers of disease, including microRNAs (miRNAs), with viral and tumor-derived miRNAs being detected in exosomes from individuals with KSHV-associated malignancies. In the current study, the host and viral extracellular mature miRNA expression profiles were analyzed in blood of KS-negative individuals in Uganda, comparing those with or without KSHV detectable from the oropharynx. We observed increased levels of cellular oncogenic miRNAs and decreased levels of tumor-suppressor miRNAs in plasma of infected individuals exhibiting oral KSHV shedding. These changes in host oncomiRs were exacerbated in people co-infected with HIV, and partially reversed after 2 years of anti-retroviral therapy. We also detected KSHV miRNAs in plasma of KSHV infected individuals and determined that their expression levels correlated with KSHV plasma viremia. Deep sequencing revealed an expected profile of small cellular RNAs in plasma, with miRNAs constituting the major RNA biotype. In contrast, the composition of small RNAs in exosomes was highly atypical with high levels of YRNA and low levels of miRNAs. Mass spectrometry analysis of the exosomes revealed eleven different peptides derived from the malaria parasite, Plasmodium falciparum, and small RNA sequencing confirmed widespread plasmodium co-infections in the Ugandan cohorts. Proteome analysis indicated an exosomal protein profile consistent with erythrocyte and keratinocyte origins for the plasma exosomes. A strong correlation was observed between the abundance of Plasmodium proteins and cellular markers of malaria. As Plasmodium falciparum is an endemic pathogen in Uganda, our study shows that co-infection with other pathogens, such as KSHV, can severely impact the small

  19. The effects of megafaunal burrows on radiotracer profiles and organic composition in deep-sea sediments: preliminary results from two sites in the bathyal north-east Atlantic

    Science.gov (United States)

    Hughes, D. J.; Brown, L.; Cook, G. T.; Cowie, G.; Gage, J. D.; Good, E.; Kennedy, H.; MacKenzie, A. B.; Papadimitriou, S.; Shimmield, G. B.; Thomson, J.; Williams, M.

    2005-01-01

    Megafaunal burrows were detected in boxcores from two sites in the bathyal north-east Atlantic. Burrow contents were analysed to assess their significance to sediment radiotracer profiles and organic composition. At 1100 m depth, burrow openings up to 3 cm diameter occurred at a density of approximately 5 m -2. Burrows at 12-18 cm sediment depth extending horizontally for up to 35 cm and linked to the surface by vertical shafts were provisionally attributed to echiuran worms, although no occupants were found in situ. In one example the horizontal burrow section was filled with green slurry, for which scanning electron microscopy, 210Pb excess and organic content all indicated a phytodetrital origin. At 1920 m depth no large burrow openings were found in five boxcores examined, but large subsurface biogenic structures were present. Galleries at 15-26 cm depth were traced horizontally for up to 30 cm, but contained no occupants or filling. Extended linear bands of faecal pellets were found in three boxcores at 13-17 cm depth. Excess 210Pb content indicated that most of these structures resulted from surface deposit feeding. Faecal pellet bands may partially explain the occurrence of subsurface peaks detected in profiles of 210Pb excess at this site. Results suggest that 'caching' of phytodetritus and subsurface deposition of faeces are two mechanisms for the rapid, deep burial of relatively fresh organic matter, but the significance of these processes to sediment geochemistry cannot be quantified without much information on the distribution, identity and abundance of burrowing megafauna in the deep sea.

  20. Geometry of the Arabia-Somalia Plate Boundary into Afar: Preliminary Results from the Seismic Profile Across the Asal Rift (Djibouti)

    Science.gov (United States)

    Vergne, J.; Doubre, C.; Mohamed, K.; Tiberi, C.; Leroy, S.; Maggi, A.

    2010-12-01

    In the Afar Depression, the Asal-Ghoubbet Rift in Djibouti is a young segment on land at the propagating tip of the Aden Ridge. This segment represents an ideal laboratory to observe the mechanisms of extension and the structural evolutions involved, from the continental break-up to the first stage of oceanic spreading. However, we lack first order information about the crustal and upper mantle structure in this region, which for example prevent detailed numerical modeling of the deformations observed at the surface from GPS or InSAR. Moreover the current permanent network is not well suited to precisely constrain the ratio of seismic/aseismic deformation and to characterize the active deformation and the rifting dynamics. Since November 2009 we have maintained a temporary network of 25 seismic stations deployed along a 150 km-long profile. Because we expect rapid variations of the lithospheric structure across the 10 km-wide central part of the rift, we gradually decreased the inter-stations spacing to less than 1 km in the middle section of the profile. In order to obtain a continuous image of the plate boundary, from the topographic surface to the upper mantle, several techniques and methods will be applied: P and S wave receiver functions, tomographies based on body waves, surface waves and seismic noise correlation, anisotropy, and finally a gravity-seismic joint inversion. We present some preliminary results deduced from the receiver functions applied to the data acquired during the first months of the experiment. We migrate several sets of receiver functions computed in various frequency bands to resolve both mantle interfaces and fine scale structures within the thin crust in the center of the rift. These first images confirm a rapid variation of the Moho depth on both sides of the rift and a very complex lithospheric structure in the central section with several low velocity zones within the top 50km that might correspond to magma lenses.

  1. Acute Toxicity Profile and Compliance to Accelerated Radiotherapy Plus Carbogen and Nicotinamide for Clinical Stage T2-4 Laryngeal Cancer: Results of a Phase III Randomized Trial

    Energy Technology Data Exchange (ETDEWEB)

    Janssens, Geert O., E-mail: g.janssens@rther.umcn.nl [Department of Radiation Oncology, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands); Terhaard, Chris H. [Department of Radiation Oncology, University Medical Center Utrecht, Utrecht (Netherlands); Doornaert, Patricia A. [Department of Radiation Oncology, VU University Medical Center, Amsterdam (Netherlands); Bijl, Hendrik P. [Department of Radiation Oncology, University Medical Center Groningen, Groningen (Netherlands); Ende, Piet van den [Department of Radiation Oncology, Maastricht University Medical Centre, Maastricht (Netherlands); Chin, Alim [Department of Clinical Oncology, Leiden University Medical Centre, Leiden (Netherlands); Pop, Lucas A.; Kaanders, Johannes H. [Department of Radiation Oncology, Radboud University Nijmegen Medical Centre, Nijmegen (Netherlands)

    2012-02-01

    Purpose: To report the acute toxicity profile and compliance from a randomized Phase III trial comparing accelerated radiotherapy (AR) with accelerated radiotherapy plus carbogen and nicotinamide (ARCON) in laryngeal cancer. Methods and Materials: From April 2001 to February 2008, 345 patients with cT2-4 squamous cell laryngeal cancer were randomized to AR (n = 174) and ARCON (n = 171). Acute toxicity was scored weekly until Week 8 and every 2-4 weeks thereafter. Compliance to carbogen and nicotinamide was reported. Results: Between both treatment arms (AR vs. ARCON) no statistically significant difference was observed for incidence of acute skin reactions (moist desquamation: 56% vs. 58%, p = 0.80), acute mucosal reactions (confluent mucositis: 79% vs. 85%, p = 0.14), and symptoms related to acute mucositis (severe pain on swallowing: 53% vs. 58%, p = 0.37; nasogastric tube feeding: 28% vs. 28%, p = 0.98; narcotic medicines required: 58% vs. 58%, p = 0.97). There was a statistically significant difference in median duration of confluent mucositis in favor of AR (2.0 vs 3.0 weeks, p = 0.01). There was full compliance with carbogen breathing and nicotinamide in 86% and 80% of the patients, with discontinuation in 6% and 12%, respectively. Adjustment of antiemesis prophylaxis was needed in 42% of patients. Conclusion: With the exception of a slight increase in median duration of acute confluent mucositis, the present data reveal a similar acute toxicity profile between both regimens and a good compliance with ARCON for clinical stage T2-4 laryngeal cancers. Treatment outcome and late morbidity will determine the real therapeutic benefit.

  2. A new method to determine cortical bone thickness in CT images using a hybrid approach of parametric profile representation and local adaptive thresholds: Accuracy results.

    Science.gov (United States)

    Museyko, Oleg; Gerner, Bastian; Engelke, Klaus

    2017-01-01

    Cortical bone is an important contributor to bone strength and is pivotal to understand the etiology of osteoporotic fractures and the specific mechanisms of antiosteoporotic treatment regimen. 3D computed tomography (CT) can be used to measure cortical thickness, density, and mass in the proximal femur, lumbar vertebrae, and distal forearm. However, the spatial resolution of clinical whole body CT scanners is limited by radiation exposure; partial volume artefacts severely impair the accurate assessment of cortical parameters, in particular in locations where the cortex is thin such as in the lumbar vertebral bodies or in the femoral neck. Model-based deconvolution approaches recover the cortical thickness by numerically deconvolving the image along 1D profiles using an estimated scanner point spread function (PSF) and a hypothesized uniform cortical bone mineral density (reference density). In this work we provide a new essentially analytical unique solution to the model-based cortex recovery problem using few characteristics of the measured profile and thus eliminate the non-linear optimization step for deconvolution. Also, the proposed approach allows to get rid of the PSF in the model and reduces sensitivity to errors in the reference density. Additionally, run-time and memory effective computation of cortical thickness was achieved with the help of a lookup table. The method accuracy and robustness was validated and compared to that of a deconvolution approach recently proposed for cortical bone and of the 50% relative threshold technique: in a simulated environment with noise and various error levels in the reference density and using CT acquisitions of the European Forearm Phantom (EFP II), a modification of a widely used anthropomorphic standard of cortical and trabecular bone compartments that was scanned with various scan protocols. Results of simulations and of phantom data analysis verified the following properties of the new method: 1) Robustness

  3. Routine molecular profiling of patients with advanced non-small-cell lung cancer: results of a 1-year nationwide programme of the French Cooperative Thoracic Intergroup (IFCT).

    Science.gov (United States)

    Barlesi, Fabrice; Mazieres, Julien; Merlio, Jean-Philippe; Debieuvre, Didier; Mosser, Jean; Lena, Hervé; Ouafik, L'Houcine; Besse, Benjamin; Rouquette, Isabelle; Westeel, Virginie; Escande, Fabienne; Monnet, Isabelle; Lemoine, Antoinette; Veillon, Rémi; Blons, Hélène; Audigier-Valette, Clarisse; Bringuier, Pierre-Paul; Lamy, Régine; Beau-Faller, Michèle; Pujol, Jean-Louis; Sabourin, Jean-Christophe; Penault-Llorca, Frédérique; Denis, Marc G; Lantuejoul, Sylvie; Morin, Franck; Tran, Quân; Missy, Pascale; Langlais, Alexandra; Milleron, Bernard; Cadranel, Jacques; Soria, Jean-Charles; Zalcman, Gérard

    2016-04-02

    The molecular profiling of patients with advanced non-small-cell lung cancer (NSCLC) for known oncogenic drivers is recommended during routine care. Nationally, however, the feasibility and effects on outcomes of this policy are unknown. We aimed to assess the characteristics, molecular profiles, and clinical outcomes of patients who were screened during a 1-year period by a nationwide programme funded by the French National Cancer Institute. This study included patients with advanced NSCLC, who were routinely screened for EGFR mutations, ALK rearrangements, as well as HER2 (ERBB2), KRAS, BRAF, and PIK3CA mutations by 28 certified regional genetics centres in France. Patients were assessed consecutively during a 1-year period from April, 2012, to April, 2013. We measured the frequency of molecular alterations in the six routinely screened genes, the turnaround time in obtaining molecular results, and patients' clinical outcomes. This study is registered with ClinicalTrials.gov, number NCT01700582. 18,679 molecular analyses of 17,664 patients with NSCLC were done (of patients with known data, median age was 64·5 years [range 18-98], 65% were men, 81% were smokers or former smokers, and 76% had adenocarcinoma). The median interval between the initiation of analysis and provision of the written report was 11 days (IQR 7-16). A genetic alteration was recorded in about 50% of the analyses; EGFR mutations were reported in 1947 (11%) of 17,706 analyses for which data were available, HER2 mutations in 98 (1%) of 11,723, KRAS mutations in 4894 (29%) of 17,001, BRAF mutations in 262 (2%) of 13,906, and PIK3CA mutations in 252 (2%) of 10,678; ALK rearrangements were reported in 388 (5%) of 8134 analyses. The median duration of follow-up at the time of analysis was 24·9 months (95% CI 24·8-25·0). The presence of a genetic alteration affected first-line treatment for 4176 (51%) of 8147 patients and was associated with a significant improvement in the proportion of patients

  4. Preliminary results from the Stereo-SCIDAR at the VLT Observatory: extraction of reference atmospheric turbulence profiles for E-ELT adaptive optics instrument performance simulations

    Science.gov (United States)

    Sarazin, Marc S.; Osborn, James; Chacon-Oelckers, Arlette; Dérie, Frédéric J.; Le Louarn, Miska; Milli, Julien; Navarrete, Julio; Wilson, Richard R. W.

    2017-09-01

    The Stereo-SCIDAR (Scintillation Detection and Ranging) atmospheric turbulence profiler, built for ESO by Durham University, observes the scintillation patterns of binary elements with one of the four VLT-Interferometer 1.8m auxiliary telescopes at the ESO Paranal Observatory. The primary products are the vertical profiles of the index of refraction structure coefficient and of the wind velocity which allow to compute the wavefront coherence time and the isoplanatic angle with a vertical resolution of 250m. The several thousands of profiles collected during more than 30 nights of operation are grouped following criteria based on the altitude distribution or on principal component analysis. A set of reference profiles representative of the site is proposed as input for the various simulation models developed by the E-ELT (European Extremely Large Telescope) instruments Consortia.

  5. Mental health promotion in a school community by using the results from the Well-being Profile: an action research project.

    Science.gov (United States)

    Puolakka, Kristiina; Haapasalo-Pesu, Kirsi-Maria; Konu, Anne; Astedt-Kurki, Päivi; Paavilainen, Eija

    2014-01-01

    This article presents an action research project as a method to combine science and practical expertise in order to develop the practices of the health care system. The project aimed at developing mental health promotion in the school community in general and at finding tools for timely help when mental health is at risk. The underlying idea is that mental health is an integral part of health and by promoting general well-being it is also possible to promote and ensure mental health at school. The study was conducted in a Finnish lower secondary school of 446 pupils where the pupils are aged between 12 and 15 years. The initial survey was conducted using the School Well-being Profile, a tool developed by Anne Konu. A well-being questionnaire was used to identify the areas in need of improvement, providing the basis for planning and implementing development measures together with the local actors. The instrument proved to be a usable way of collecting feedback of the well-being of the school environment. As a result of the action research project, the school's physical conditions and social relationships improved and appropriate practices for future problem situations were set.

  6. A new method to determine cortical bone thickness in CT images using a hybrid approach of parametric profile representation and local adaptive thresholds: Accuracy results.

    Directory of Open Access Journals (Sweden)

    Oleg Museyko

    Full Text Available Cortical bone is an important contributor to bone strength and is pivotal to understand the etiology of osteoporotic fractures and the specific mechanisms of antiosteoporotic treatment regimen. 3D computed tomography (CT can be used to measure cortical thickness, density, and mass in the proximal femur, lumbar vertebrae, and distal forearm. However, the spatial resolution of clinical whole body CT scanners is limited by radiation exposure; partial volume artefacts severely impair the accurate assessment of cortical parameters, in particular in locations where the cortex is thin such as in the lumbar vertebral bodies or in the femoral neck.Model-based deconvolution approaches recover the cortical thickness by numerically deconvolving the image along 1D profiles using an estimated scanner point spread function (PSF and a hypothesized uniform cortical bone mineral density (reference density. In this work we provide a new essentially analytical unique solution to the model-based cortex recovery problem using few characteristics of the measured profile and thus eliminate the non-linear optimization step for deconvolution. Also, the proposed approach allows to get rid of the PSF in the model and reduces sensitivity to errors in the reference density. Additionally, run-time and memory effective computation of cortical thickness was achieved with the help of a lookup table.The method accuracy and robustness was validated and compared to that of a deconvolution approach recently proposed for cortical bone and of the 50% relative threshold technique: in a simulated environment with noise and various error levels in the reference density and using CT acquisitions of the European Forearm Phantom (EFP II, a modification of a widely used anthropomorphic standard of cortical and trabecular bone compartments that was scanned with various scan protocols.Results of simulations and of phantom data analysis verified the following properties of the new method: 1

  7. Controlled type II diabetes mellitus has no major influence on platelet micro-RNA expression. Results from micro-array profiling in a cohort of 60 patients.

    Science.gov (United States)

    Stratz, Christian; Nührenberg, Thomas; Fiebich, Bernd L; Amann, Michael; Kumar, Asit; Binder, Harald; Hoffmann, Isabell; Valina, Christian; Hochholzer, Willibald; Trenk, Dietmar; Neumann, Franz-Josef

    2014-05-05

    Diabetes mellitus as a major contributor to cardiovascular disease burden induces dysfunctional platelets. Platelets contain abundant miRNAs, which are linked to inflammatory responses and, thus, may play a role in atherogenesis. While diabetes mellitus affects plasma miRNAs, no data exist on platelet miRNA profiles in this disease. Therefore, this study sought to explore the miRNA profile of platelets in patients with diabetes mellitus that is unrelated to the presence or absence of coronary artery disease (CAD). Platelet miRNA profiles were assessed in stable diabetic and non-diabetic patients (each n=30); 15 patients in each group had CAD. Platelet miRNA was isolated from leucocyte-depleted platelet-rich plasma, and miRNA profiling was performed using LNA micro-array technology (miRBase18.0, containing 1,917 human miRNAs). Effects of diabetes mellitus were explored by univariate statistical tests for each miRNA, adjusted for potential confounders, and by developing a multivariable signature; evaluated by resampling techniques. Platelets in non-diabetic patients demonstrated miRNA expression profiles comparable to previous data. The miRNA profiles of platelets in diabetics were similar. Statistical analysis unveiled three miRNAs (miR-377-5p, miR-628-3p, miR-3137) with high reselection probabilities in resampling techniques, corresponding to signatures with modest discriminatory performance. Functional annotation of predicted targets for these miRNAs pointed towards an influence of diabetes mellitus on mRNA processing. We did not find major differences in platelet miRNA profiles between diabetics and non-diabetics. Minor differences pertained to miRNAs associated with mRNA processing. Thus, described differences in plasma miRNAs between diabetic and non-diabetic patients cannot be explained by plain changes in platelet miRNA profile.

  8. Analysis of the VCX3A, VCX2 and VCX3B genes shows that VCX3A gene deletion is not sufficient to result in mental retardation in X-linked ichthyosis.

    Science.gov (United States)

    Cuevas-Covarrubias, S A; González-Huerta, L M

    2008-03-01

    X-linked ichthyosis (XLI), an inborn error of metabolism, is due to steroid sulphatase (STS) deficiency. Most patients with XLI harbour complete deletion of the STS gene and flanking sequences. The presence of low copy number repeats on either side of the STS gene seems to have a major role in the high frequency of these deletions. Some patients with XLI with terminal deletions of Xp22.3 involving marker DXS1139 and the STS gene show mental retardation (MR); VCX3A is the only gene located on this critical region. To analyse the VCX3A, VCX, VCX2 and VCX3B genes in 80 unrelated Mexican patients with XLI with normal intelligence. STS activity was measured in the leucocytes using 7-[3H]-dehydroepiandrosterone sulphate as a substrate. Amplification of the regions from telomeric DXS89 to centromeric DXS1134 including both extremes of the STS and the VCX3A, VCX, VCX2 and VCX3B genes was performed using polymerase chain reaction. No STS activity was detected in the patients with XLI (0.00 pmol mg(-1) protein h(-1)). We observed two different deletion patterns: the first group included 62 patients with deletion of VCX3A and VCX genes. The second group included 18 patients with breakpoints at several regions on either side of the STS gene not including the VCX3A gene. These data indicate that more complex mechanisms, apart from possible VCX3A gene participation, are occurring in the genesis of MR in XLI, at least in the sample of Mexican patients analysed.

  9. SELECTED MACROECONOMIC FACTORS OBSERVATION IMPACT ON THE ECONOMY OF THE REPUBLIC OF SERBIA AND THE WESTERN BALKANS IN THE OBSERVATION PERIOD 2000-2012, SHOWING RESULTS FROM 2008 TO 2012.

    Directory of Open Access Journals (Sweden)

    BOGDAN LABAN

    2017-12-01

    Full Text Available The application of the neoliberal concept in the Western Balkans has had effects on the economies of those countries that can say das resulted in unsustainable economic growth and macroeconomic instability. Besides the application of the same given the developments that had features of weakness especially since 2008., from the onset of the global economic crisis. The consequences of the liberalization of international economic developments have enabled the countries of the Western Balkans obtain foreign investment in the form of foreign direct investment, but their economies have done even more dependent on foreign capital, which is increasingly going in economic activities that have a faster way could fertilize the invested capital.

  10. The "Life Potential": a new complex algorithm to assess "Heart Rate Variability" from Holter records for cognitive and diagnostic aims. Preliminary experimental results showing its dependence on age, gender and health conditions

    CERN Document Server

    Barra, Orazio A

    2013-01-01

    Although HRV (Heart Rate Variability) analyses have been carried out for several decades, several limiting factors still make these analyses useless from a clinical point of view. The present paper aims at overcoming some of these limits by introducing the "Life Potential" (BMP), a new mathematical algorithm which seems to exhibit surprising cognitive and predictive capabilities. BMP is defined as a linear combination of five HRV Non-Linear Variables, in turn derived from the thermodynamic formalism of chaotic dynamic systems. The paper presents experimental measurements of BMP (Average Values and Standard Deviations) derived from 1048 Holter tests, matched in age and gender, including a control group of 356 healthy subjects. The main results are: (a) BMP always decreases when the age increases, and its dependence on age and gender is well established; (b) the shape of the age dependence within "healthy people" is different from that found in the general group: this behavior provides evidence of possible illn...

  11. Behavioral Risk Profile of Men Who Have Sex with Men in Beijing, China: Results from a Cross-sectional Survey with Randomized Response Techniques

    Directory of Open Access Journals (Sweden)

    Guo-Zhu Geng

    2016-01-01

    Conclusions: Our study employed RRTs in a survey containing questions on sexual behavior among MSM, and the results showed that RRT might be a useful tool to obtain truthful feedback on sensitive information such as sexual behavior from the respondents, especially in traditional Chinese cultural settings.

  12. Cholesterol testing and results

    Science.gov (United States)

    Cholesterol test results; LDL test results; VLDL test results; HDL test results; Coronary risk profile results; Hyperlipidemia-results; Lipid disorder test results; Heart disease - cholesterol results

  13. Nifedipine plus candesartan combination increases blood pressure control regardless of race and improves the side effect profile: DISTINCT randomized trial results.

    Science.gov (United States)

    Kjeldsen, Sverre E; Sica, Domenic; Haller, Hermann; Cha, Gloria; Gil-Extremera, Blas; Harvey, Peter; Heyvaert, Frank; Lewin, Andrew J; Villa, Giuseppe; Mancia, Giuseppe

    2014-12-01

    DISTINCT (reDefining Intervention with Studies Testing Innovative Nifedipine GITS - Candesartan Therapy) aimed to determine the dose-response and tolerability of nifedipine GITS and/or candesartan cilexetil therapy in participants with hypertension. In this 8-week, multinational, multicentre, randomized, double-blind, placebo-controlled study, adults with mean seated DBP of at least 95 to less than 110 mmHg received combination or monotherapy with nifedipine GITS (N) 20, 30 or 60 mg and candesartan cilexetil (C) 4, 8, 16 or 32 mg, or placebo. The primary endpoint, change in DBP from baseline to Week 8, was analysed using the response surface model (RSM); this analysis was repeated for mean seated SBP. Overall, 1381 participants (mean baseline SBP/DBP: 156.5/99.6 mmHg) were randomized. Both N and C contributed independently to SBP/DBP reductions [P < 0.0001 (RSM)]. A positive dose-response was observed, with all combinations providing statistically better blood pressure (BP) reductions from baseline versus respective monotherapies (P < 0.05) and N60C32 achieving the greatest reduction [-23.8/-16.5 mmHg; P < 0.01 versus placebo (-5.3/-6.7 mmHg) and component monotherapies]. Even very low-dose (N20 and C4) therapy provided significant BP-lowering, and combination therapy was similarly effective in different racial groups. N/C combination demonstrated a lower incidence of vasodilatory adverse events than N monotherapy (18.3 versus 23.6%), including headache (5.5 versus 11.0%; P = 0.003, chi-square test) and peripheral oedema over time (3.6 versus 5.8%; n.s.). N/C combination was effective in participants with hypertension and showed an improved side effect profile compared with N monotherapy.

  14. Evaluation of real-time data obtained from gravimetric preparation of antineoplastic agents shows medication errors with possible critical therapeutic impact: Results of a large-scale, multicentre, multinational, retrospective study.

    Science.gov (United States)

    Terkola, R; Czejka, M; Bérubé, J

    2017-08-01

    Medication errors are a significant cause of morbidity and mortality especially with antineoplastic drugs, owing to their narrow therapeutic index. Gravimetric workflow software systems have the potential to reduce volumetric errors during intravenous antineoplastic drug preparation which may occur when verification is reliant on visual inspection. Our aim was to detect medication errors with possible critical therapeutic impact as determined by the rate of prevented medication errors in chemotherapy compounding after implementation of gravimetric measurement. A large-scale, retrospective analysis of data was carried out, related to medication errors identified during preparation of antineoplastic drugs in 10 pharmacy services ("centres") in five European countries following the introduction of an intravenous workflow software gravimetric system. Errors were defined as errors in dose volumes outside tolerance levels, identified during weighing stages of preparation of chemotherapy solutions which would not otherwise have been detected by conventional visual inspection. The gravimetric system detected that 7.89% of the 759 060 doses of antineoplastic drugs prepared at participating centres between July 2011 and October 2015 had error levels outside the accepted tolerance range set by individual centres, and prevented these doses from reaching patients. The proportion of antineoplastic preparations with deviations >10% ranged from 0.49% to 5.04% across sites, with a mean of 2.25%. The proportion of preparations with deviations >20% ranged from 0.21% to 1.27% across sites, with a mean of 0.71%. There was considerable variation in error levels for different antineoplastic agents. Introduction of a gravimetric preparation system for antineoplastic agents detected and prevented dosing errors which would not have been recognized with traditional methods and could have resulted in toxicity or suboptimal therapeutic outcomes for patients undergoing anticancer treatment.

  15. Character profiles and life satisfaction.

    Science.gov (United States)

    Park, Hwanjin; Suh, Byung Seong; Kim, Won Sool; Lee, Hye-Kyung; Park, Seon-Cheol; Lee, Kounseok

    2015-04-01

    There is a surge of interest in subjective well-being (SWB), which concerns how individuals feel about their happiness. Life satisfaction tends to be influenced by individual psychological traits and external social factors. The aim of this study was to examine the relationship between individual character and SWB. Data from 3522 university students were analyzed in this study. Character profiles were evaluated using the Temperament and Character Inventory-Revised Short version (TCI-RS). Life satisfaction was assessed using the Satisfaction with Life Scale (SWLS). All statistical tests regarding the correlations between each character profile and life satisfaction were conducted using ANOVAs, t-tests, multiple linear regression models and correlation analyses. The creative (SCT) profile was associated with the highest levels of life satisfaction, whereas the depressive (sct) profile was associated with the lowest levels of life satisfaction. Additionally, high self-directedness, self-transcendence and cooperation were associated with high life satisfaction. The results of gender-adjusted multiple regression analysis showed that the effects of self-directedness were the strongest in the assessment of one's quality of life, followed by self-transcendence and cooperativeness, in that order. All of the three-character profiles were significantly correlated with one's quality of life, and the character profiles of TCI-RS explained 27.6% of life satisfaction in total. Among the three-character profiles, the self-directedness profile was most associated with life satisfaction. Our study was cross-sectional, and self-reported data from students at a single university were analyzed. The results of this study showed that, among the character profiles, the effects of self-directedness were the strongest for predicting life satisfaction. Copyright © 2014 Elsevier Inc. All rights reserved.

  16. Natural current profiles in a tokamak

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, J.B.

    1990-08-01

    In this paper I show how one may arrive at a universal, or natural, family of Tokamak profiles using only accepted physical principles. These particular profiles are similar to ones proposed previously on the basis of ad hoc variational principles and the point of the present paper is to provide a justification for them. However in addition, the present work provides an interesting view of Tokamak fluctuations and leads to a new result -- a relationship between the inward particle pinch velocity, the diffusion coefficient and the current profile. The basic Tokamak model is described in this paper. Then an analogy is developed between Tokamak profiles and the equilibrium of a realisable dynamical system. Then the equations governing the natural Tokamak profiles are derived by applying standard statistical mechanics to this analog. The profiles themselves are calculated and some other results of the theory are described.

  17. Statistical Correlations between HPLC Activity-Based Profiling Results and NMR/MS Microfraction Data to Deconvolute Bioactive Compounds in Mixtures

    Directory of Open Access Journals (Sweden)

    Samuel Bertrand

    2016-02-01

    Full Text Available Recent approaches in natural product (NP research are leading toward the discovery of bioactive chemical entities at the microgram level. In comparison to classical large scale bioassay-guided fractionation, the use of LC-MS metabolite profiling in combination with microfractionation for both bioactivity profiling and NMR analysis, allows the identification of bioactive compounds at a very early stage. In that context, this study aims to assess the potential of statistic correlation analysis to enable unambiguous identification of features related to bioactive compounds in mixtures, without the need for complete isolation. For that purpose, a mixture of NPs was microfractionated by rapid small-scale semi-preparative HPLC for proof-of-concept. UHPLC-ESI-TOFMS profiles, micro-flow CapNMR spectra and a cancer chemopreventive assay carried out on every microfraction were analysed by statistical correlations.

  18. Stem cells show promising results for lymphoedema treatment

    DEFF Research Database (Denmark)

    Toyserkani, Navid Mohamadpour; Quaade, Marlene Louise; Sheikh, Søren Paludan

    2015-01-01

    Abstract Lymphoedema is a debilitating condition, manifesting in excess lymphatic fluid and swelling of subcutaneous tissues. Lymphoedema is as of yet still an incurable condition and current treatment modalities are not satisfactory. The capacity of mesenchymal stem cells to promote angiogenesis......, secrete growth factors, regulate the inflammatory process, and differentiate into multiple cell types make them a potential ideal therapy for lymphoedema. Adipose tissue is the richest and most accessible source of mesenchymal stem cells and they can be harvested, isolated, and used for therapy...... in a single stage procedure as an autologous treatment. The aim of this paper was to review all studies using mesenchymal stem cells for lymphoedema treatment with a special focus on the potential use of adipose-derived stem cells. A systematic search was performed and five preclinical and two clinical...

  19. Buried volcanic structures in the Gulf of Naples (Southern Tyrrhenian Sea, Italy resulting from high resolution magnetic survey and seismic profiling

    Directory of Open Access Journals (Sweden)

    S. Ruggieri

    2005-06-01

    Full Text Available In this paper we present a correlation between volcanic structures and magnetic anomalies in the Gulf of Naples (Southern Tyrrhenian Sea based on high resolution magnetic profiling. A densely spaced grid of magnetic profiles coupled with multichannel seismics (seismic source Watergun 15 cubic inch was recorded in the Gulf of Naples, representing an active volcanic area during the Late Quaternary (volcanic centers of Somma-Vesuvius, Phlegraean Fields and Ischia and Procida islands. The dataset was collected during the oceanographic cruise GMS00-05 which took place during October-November 2000 in the South Tyrrhenian Sea onboard of the R/V Urania (National Research Council, Italy. Shallow volcanic structures in the subsurface of the gulf were recognized by seismo-stratigraphic analysis of high resolution profiles; the volcanic nature of some of these structures was inferred identifying the magnetic anomalies on a high resolution magnetic anomaly map of the gulf. Even if qualitative, the correlations between seismic and magnetic profiles allow us to better assess the geological structure of the Gulf of Naples.

  20. ACUTE TOXICITY PROFILE AND COMPLIANCE TO ACCELERATED RADIOTHERAPY PLUS CARBOGEN AND NICOTINAMIDE FOR CLINICAL STAGE T2-4 LARYNGEAL CANCER : RESULTS OF A PHASE III RANDOMIZED TRIAL

    NARCIS (Netherlands)

    Janssens, Geert O.; Terhaard, Chris H.; Doornaert, Patricia A.; Bijl, Hendrik P.; van den Ende, Piet; Chin, Alim; Pop, Lucas A.; Kaanders, Johannes H.

    2012-01-01

    Purpose: To report the acute toxicity profile and compliance from a randomized Phase III trial comparing accelerated radiotherapy (AR) with accelerated radiotherapy plus carbogen and nicotinamide (ARCON) in laryngeal cancer. Methods and Materials: From April 2001 to February 2008, 345 patients with

  1. Acute toxicity profile and compliance to accelerated radiotherapy plus carbogen and nicotinamide for clinical stage T2-4 laryngeal cancer: results of a phase III randomized trial.

    NARCIS (Netherlands)

    Janssens, G.O.R.J.; Terhaard, C.H.J.; Doornaert, P.A.; Bijl, H.P.; Ende, P. van den; Chin, A.; Pop, L.A.M.; Kaanders, J.H.A.M.

    2012-01-01

    PURPOSE: To report the acute toxicity profile and compliance from a randomized Phase III trial comparing accelerated radiotherapy (AR) with accelerated radiotherapy plus carbogen and nicotinamide (ARCON) in laryngeal cancer. METHODS AND MATERIALS: From April 2001 to February 2008, 345 patients with

  2. Latent profile analysis of students’ motivation and outcomes in mathematics: an organismic integration theory perspective

    Directory of Open Access Journals (Sweden)

    Chee Keng John Wang

    2017-05-01

    Full Text Available The purpose of the current study was to identify the motivation profiles at the intraindividual level using a latent profile analyses (LPA approach. A total of 1151 secondary school students aged 13 to 17 years old from Singapore took part in the study. Using LPA, four distinct motivational profiles were identified based on four motivation regulations. Profile 1 has very low introjected and low autonomous motivation (6% of sample. Profile 2 had high external and identified regulations and very low intrinsic regulation (10%. Profile 3 consisted of students with high identified and intrinsic regulations (51%. Profile 4 had moderately low identified and intrinsic regulations (33%. The results showed that the four profiles differed significantly in terms of effort, competence, value, and time spent on math beyond homework. The best profile (Profile 3 reported highest scores in effort, value, competence and time spent on Math beyond homework. The worst profile (Profile 1 reported lowest scores in all the four outcome variables.

  3. "Would you accept having your DNA profile inserted in the National Forensic DNA database? Why?" Results of a questionnaire applied in Portugal.

    Science.gov (United States)

    Machado, Helena; Silva, Susana

    2014-01-01

    The creation and expansion of forensic DNA databases might involve potential threats to the protection of a range of human rights. At the same time, such databases have social benefits. Based on data collected through an online questionnaire applied to 628 individuals in Portugal, this paper aims to analyze the citizens' willingness to donate voluntarily a sample for profiling and inclusion in the National Forensic DNA Database and the views underpinning such a decision. Nearly one-quarter of the respondents would indicate 'no', and this negative response increased significantly with age and education. The overriding willingness to accept the inclusion of the individual genetic profile indicates an acknowledgement of the investigative potential of forensic DNA technologies and a relegation of civil liberties and human rights to the background, owing to the perceived benefits of protecting both society and the individual from crime. This rationale is mostly expressed by the idea that all citizens should contribute to the expansion of the National Forensic DNA Database for reasons that range from the more abstract assumption that donating a sample for profiling would be helpful in fighting crime to the more concrete suggestion that everyone (criminals and non-criminals) should be in the database. The concerns with the risks of accepting the donation of a sample for genetic profiling and inclusion in the National Forensic DNA Database are mostly related to lack of control and insufficient or unclear regulations concerning safeguarding individuals' data and supervising the access and uses of genetic data. By providing an empirically-grounded understanding of the attitudes regarding willingness to donate voluntary a sample for profiling and inclusion in a National Forensic DNA Database, this study also considers the citizens' perceived benefits and risks of operating forensic DNA databases. These collective views might be useful for the formation of international common

  4. Clinical and Demographic Profile of Patients Receiving Fingolimod in Clinical Practice in Germany and the Benefit-Risk Profile of Fingolimod After 1 Year of Treatment: Initial Results From the Observational, Noninterventional Study PANGAEA.

    Science.gov (United States)

    Ziemssen, Tjalf; Lang, Michael; Tackenberg, Björn; Schmidt, Stephan; Albrecht, Holger; Klotz, Luisa; Haas, Judith; Lassek, Christoph; Medin, Jennie; Cornelissen, Christian

    2017-12-22

    The population with multiple sclerosis receiving treatment in clinical practice differs from that in randomized controlled trials (RCTs). An assessment of the real-world benefit-risk profile of therapies is needed. This analysis used data from the large, noninterventional, observational German study Post-Authorization Non-interventional German sAfety study of GilEnyA (PANGAEA) to assess prospectively baseline characteristics and outcomes after 12 months (± 90 days) of fingolimod treatment. Patients were divided into 2 cohorts: fingolimod starter [first received fingolimod in PANGAEA (n = 3315)] and previous study [received fingolimod before enrollment in PANGAEA in RCTs (n = 875), some of whom also had baseline data at entry into RCTs (n = 505)]. At PANGAEA baseline, patients in the fingolimod starter versus the previous study cohort had a higher annualized relapse rate [ARR (95% confidence interval): 1.79 (1.75-1.83) vs 1.32 (1.25-1.40)] and Expanded Disability Status Scale score [3.11 (3.04-3.17) vs 2.55 (2.44-2.66)]. A greater proportion in the fingolimod starter versus previous study cohort had diabetes (2.0% vs 0.7%). After 12 months of fingolimod, ARRs were lower than in the 12 months before PANGAEA enrollment in the fingolimod starter [0.386 (0.360-0.414)] and previous study [0.276 (0.238-0.320)] cohorts. Expanded Disability Status Scale scores were stable versus baseline. Adverse events were experienced by similar proportions in both cohorts during fingolimod treatment. Relevant differences exist in disease activity and comorbidities between patients receiving fingolimod in clinical practice versus RCTs. Irrespective of baseline differences indicating a higher proportion at an advanced stage of multiple sclerosis in the real world versus RCTs, fingolimod remains effective, with a manageable safety profile.

  5. Study on Rail Profile Optimization Based on the Nonlinear Relationship between Profile and Wear Rate

    Directory of Open Access Journals (Sweden)

    Jianxi Wang

    2017-01-01

    Full Text Available This paper proposes a rail profile optimization method that takes account of wear rate within design cycle so as to minimize rail wear at the curve in heavy haul railway and extend the service life of rail. Taking rail wear rate as the object function, the vertical coordinate of rail profile at range optimization as independent variable, and the geometric characteristics and grinding depth of rail profile as constraint conditions, the support vector machine regression theory was used to fit the nonlinear relationship between rail profile and its wear rate. Then, the profile optimization model was built. Based on the optimization principle of genetic algorithm, the profile optimization model was solved to achieve the optimal rail profile. A multibody dynamics model was used to check the dynamic performance of carriage running on optimal rail profile. The result showed that the average relative error of support vector machine regression model remained less than 10% after a number of training processes. The dynamic performance of carriage running on optimized rail profile met the requirements on safety index and stability. The wear rate of optimized profile was lower than that of standard profile by 5.8%; the allowable carrying gross weight increased by 12.7%.

  6. Efficacy and safety profile of a compound composed of platelet-rich plasma and hyaluronic acid in the treatment for knee osteoarthritis (preliminary results).

    Science.gov (United States)

    Abate, Michele; Verna, Sandra; Schiavone, Cosima; Di Gregorio, Patrizia; Salini, Vincenzo

    2015-12-01

    The combined use of hyaluronic acid and platelet-rich plasma has never been reported in the treatment for osteoarthritis. Aim of this paper was to evaluate the efficacy of this association and to compare retrospectively these results with those of a cohort of patients treated with platelet-rich plasma only. Subjects with mild-to-moderate knee osteoarthritis were enrolled. After clinical and ultrasound evaluation, patients received a weekly intra-articular injection of 2 ml of hyaluronic acid added with 2 ml of platelet-rich plasma for 3 weeks. Follow-up was performed at 1, 3, and 6 months. The same clinical parameters were retrospectively collected from a cohort of patients treated with 4-5 ml of platelet-rich plasma only. Forty knees were treated in both groups. The intra-group comparison showed a significant improvement in clinical and functional outcomes at 1, 3, and 6 months, while the infra-group comparison did not show any significant difference. The association of platelet-rich plasma + hyaluronic acid has the same efficacy of platelet-rich plasma only, administered in higher volume. We may infer that hyaluronic acid works synergically and improves the activity of several molecules contained in platelet-rich plasma.

  7. MPI Profiling

    Energy Technology Data Exchange (ETDEWEB)

    Han, D K; Jones, T R

    2005-02-11

    The Message Passing Interface (MPI) is the de facto message-passing standard for massively parallel programs. It is often the case that application performance is a crucial factor, especially for solving grand challenge problems. While there have been many studies on the scalability of applications, there have not been many focusing on the specific types of MPI calls being made and their impact on application performance. Using a profiling tool called mpiP, a large spectrum of parallel scientific applications were surveyed and their performance results analyzed.

  8. The effects of rituximab on the lipid profile of patients with active systemic lupus erythematosus: results from a nationwide cohort in Spain (LESIMAB).

    Science.gov (United States)

    Fernández-Nebro, A; Marenco, J L; López-Longo, F; Galindo, M; Hernández-Cruz, B E; Narváez, J; Rúa-Figueroa, I; Raya-Alvarez, E; Zea, A; Freire, M; Sánchez-Atrio, A I; García-Vicuña, R; Pego-Reigosa, J M; Manrique-Arija, S; Nieves-Martín, L; Carreño, L

    2014-09-01

    Patients with systemic lupus erythematosus (SLE) have increased cardiovascular risk related to lipid changes induced by inflammatory activity, proteinuria and treatments. Our objective was to analyse lipid changes in a cohort of patients with SLE resistant to standard treatments who were treated with rituximab. The study population comprised a retrospective multicentre, national cohort of patients with SLE resistant to standard treatments who were treated with rituximab. The basic lipid profile, concomitant treatment and disease activity were analysed at the start of the treatment, 24 weeks later, and at the end of the follow-up period. The effects of the main lupus variables and therapy on the lipid changes were analysed. Seventy-nine patients with active lupus treated with rituximab were assessed during 149.3 patient-years. Prior to the treatment, 69% had dyslipidaemia. The most frequent abnormalities were a low-density lipoprotein (LDL) level of ≥100 mg/dl (34%) and a high-density lipoprotein (HDL) level of lipid profile of patients with SLE refractory to standard treatment, mainly by reducing inflammatory activity. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

  9. Testing the Capacity of a Multi-Nutrient Profiling System to Guide Food and Beverage Reformulation: Results from Five National Food Composition Databases

    Directory of Open Access Journals (Sweden)

    Emilie Combet

    2017-04-01

    Full Text Available Nutrient profiling ranks foods based on their nutrient composition, with applications in multiple aspects of food policy. We tested the capacity of a category-specific model developed for product reformulation to improve the average nutrient content of foods, using five national food composition datasets (UK, US, China, Brazil, France. Products (n = 7183 were split into 35 categories based on the Nestlé Nutritional Profiling Systems (NNPS and were then classified as NNPS ‘Pass’ if all nutrient targets were met (energy (E, total fat (TF, saturated fat (SFA, sodium (Na, added sugars (AS, protein, calcium. In a modelling scenario, all NNPS Fail products were ‘reformulated’ to meet NNPS standards. Overall, a third (36% of all products achieved the NNPS standard/pass (inter-country and inter-category range: 32%–40%; 5%–72%, respectively, with most products requiring reformulation in two or more nutrients. The most common nutrients to require reformulation were SFA (22%–44% and TF (23%–42%. Modelled compliance with NNPS standards could reduce the average content of SFA, Na and AS (10%, 8% and 6%, respectively at the food supply level. Despite the good potential to stimulate reformulation across the five countries, the study highlights the need for better data quality and granularity of food composition databases.

  10. Testing the Capacity of a Multi-Nutrient Profiling System to Guide Food and Beverage Reformulation: Results from Five National Food Composition Databases.

    Science.gov (United States)

    Combet, Emilie; Vlassopoulos, Antonis; Mölenberg, Famke; Gressier, Mathilde; Privet, Lisa; Wratten, Craig; Sharif, Sahar; Vieux, Florent; Lehmann, Undine; Masset, Gabriel

    2017-04-21

    Nutrient profiling ranks foods based on their nutrient composition, with applications in multiple aspects of food policy. We tested the capacity of a category-specific model developed for product reformulation to improve the average nutrient content of foods, using five national food composition datasets (UK, US, China, Brazil, France). Products ( n = 7183) were split into 35 categories based on the Nestlé Nutritional Profiling Systems (NNPS) and were then classified as NNPS 'Pass' if all nutrient targets were met (energy (E), total fat (TF), saturated fat (SFA), sodium (Na), added sugars (AS), protein, calcium). In a modelling scenario, all NNPS Fail products were 'reformulated' to meet NNPS standards. Overall, a third (36%) of all products achieved the NNPS standard/pass (inter-country and inter-category range: 32%-40%; 5%-72%, respectively), with most products requiring reformulation in two or more nutrients. The most common nutrients to require reformulation were SFA (22%-44%) and TF (23%-42%). Modelled compliance with NNPS standards could reduce the average content of SFA, Na and AS (10%, 8% and 6%, respectively) at the food supply level. Despite the good potential to stimulate reformulation across the five countries, the study highlights the need for better data quality and granularity of food composition databases.

  11. Efficacy, patient-reported outcomes and safety profile of ATX-101 (deoxycholic acid), an injectable drug for the reduction of unwanted submental fat: results from a phase III, randomized, placebo-controlled study.

    Science.gov (United States)

    Ascher, B; Hoffmann, K; Walker, P; Lippert, S; Wollina, U; Havlickova, B

    2014-12-01

    Unwanted submental fat (SMF) may result in an unattractive chin profile and dissatisfaction with appearance. An approved and rigorously tested non-surgical method for SMF reduction is lacking. To evaluate the efficacy and safety of ATX-101 for the pharmacological reduction of unwanted SMF in a phase III randomized, double-blind, placebo-controlled study. Patients (n = 360) with moderate or severe SMF were randomized to receive ATX-101 1 or 2 mg/cm(2) or placebo injected into their SMF for up to four treatments ~28 days apart, with a 12-week follow-up. Coprimary efficacy endpoints were the proportions of treatment responders, defined as a ≥1-point reduction in SMF on the Clinician-Reported Submental Fat Rating Scale (CR-SMFRS), and those satisfied with their appearance in association with their face and chin after treatment on the Subject Self-Rating Scale (SSRS score ≥4). Secondary efficacy endpoints included a ≥1-point improvement in SMF on the Patient-Reported Submental Fat Rating Scale (PR-SMFRS) and changes in the Patient-Reported Submental Fat Impact Scale (PR-SMFIS). Additional patient-reported outcomes and changes in the Skin Laxity Rating Scale were recorded. Adverse events (AEs) and laboratory test results were monitored. Compared with placebo, a greater proportion of patients treated with ATX-101 1 and 2 mg/cm(2) showed a ≥1-point improvement in CR-SMFRS (58.3% and 62.3%, respectively, vs. 34.5% with placebo; P ATX-101 1 mg/cm(2) , P ATX-101 2 mg/cm(2) vs. placebo) and emotions and perceived self-image (PR-SMFIS; P ATX-101 was effective and well tolerated, and may be an alternative to surgery for patients desiring improvement of their submental profile. © 2014 The Authors Journal of the European Academy of Dermatology and Venereology published by John Wiley & Sons Ltd on behalf of the European Academy of Dermatology and Venereology.

  12. Longitudinal analysis of cardiovascular disease risk profile in neighbourhood poverty subgroups: 5-year results from an afterschool fitness programme in the USA.

    Science.gov (United States)

    D'Agostino, Emily M; Patel, Hersila H; Hansen, Eric; Mathew, M Sunil; Nardi, Maria; Messiah, Sarah E

    2017-11-24

    The WHO calls for affordable population-based prevention strategies for reducing the global burden of cardiovascular disease (CVD) on morbidity and mortality; however, effective, sustainable and accessible community-based approaches for CVD prevention in at-risk youth have yet to be identified. We examined the effects of implementing a daily park-based afterschool fitness programme on youth CVD risk profiles over 5 years and across area poverty subgroups. The study included 2264 youth (mean age 9.4 years, 54% male, 50% Hispanic, 47% non-Hispanic black, 70% high/very high area poverty) in Miami, Florida, USA. We used three-level repeated measures mixed models to determine the longitudinal effects of programme participation on modifiable CVD outcomes (2010-2016). Duration of programme participation was significantly associated with CVD risk profile improvements, including body mass index (BMI) z-score, diastolic/systolic blood pressure, skinfold thicknesses, waist-hip ratio, sit-ups, push-ups, Progressive Aerobic Cardiovascular Endurance Run (PACER) score, 400 m run time, probability of developing systolic/diastolic hypertension and overweight/obesity in high/very high poverty neighbourhoods (P<0.001). Diastolic blood pressure decreased 3.4 percentile points (95% CI -5.85 to -0.85), 8.1 percentile points (95% CI -11.98 to -4.26), 6.1 percentile points (95% CI -11.49 to -0.66), 7.6 percentile points (95% CI -15.33 to -0.15) and 11.4 percentile points (95% CI -25.32 to 2.61) for 1-5 years, respectively, in high/very high poverty areas. In contrast, significant improvements were found only for PACER score and waist-hip ratio in low/mid poverty areas. This analysis presents compelling evidence demonstrating that park-based afterschool programmes can successfully maintain or improve at-risk youth CVD profiles over multiple years. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. No

  13. 2-μm Coherent DIAL for CO2, H2O and Wind Field Profiling in the Lower Atmosphere: Instrumentation and Results

    Directory of Open Access Journals (Sweden)

    Gibert Fabien

    2016-01-01

    Full Text Available We report on 2-μm coherent differential absorption lidar (CDIAL measurements of carbon dioxide (CO2, water vapour (H2O absorption and wind field profiling in the atmospheric boundary layer. The CDIAL uses a Tm:fiber pumped, single longitudinal mode Q-switched seeded Ho:YLF laser and a fibercoupled coherent detection. The laser operates at a pulse repetition frequency of 2 kHz and emits an output energy of 10 mJ with a pulse width of 40 ns (FWHM. Experimental horizontal and vertical range-resolved measurements were made in the atmospheric boundary layer and compared to colocated in-situ sensor data.

  14. Emergency contraceptive pill safety profile. Comparison of the results of a follow-up study to those coming from spontaneous reporting.

    Science.gov (United States)

    Carvajal, Alfonso; Sáinz, María; Velasco, Verónica; García Ortega, Pilar; Treceño, Carlos; Martín Arias, Luis H; Pellón, María; García Sevillano, Luis

    2015-01-01

    The emergency contraceptive pill (ECP) containing levonorgestrel is dispensed without a prescription in Spain since 2009. An easy access could diminish unwanted pregnancies; however, there is a risk of misuse and, in any case, of developing some adverse events. The aim of the present study is to further learn the adverse effects of this ECP. An ad hoc follow-up study was carried out in three community pharmacies in a city of Central Spain; the sample was composed of those women asking for the ECP; they were interviewed by telephone after at least a month since the last menses. We completed the safety profile obtained with that coming from spontaneous reporting in Spain. Out of 139 women surveyed, 113 developed any adverse event--two considered as severe; the most frequently reported events were menstrual disturbances, which accounted for 21% of all events. Through spontaneous reporting, 36 cases of whatever adverse events related to levonorgestrel as ECP were identified. Twenty-five cases were considered as severe. Both types of reaction and severity were significantly different in the follow-up study and in the spontaneous reporting. Some of the reactions identified, such as miscarriage, febrile neutropenia, and porphyria, are not included in the Summary of Product Characteristics. Levonorgestrel as an ECP is mostly safe. Attention should be paid to some severe events and particularly to those risk factors for them to appear. Combining spontaneous reporting with an ad hoc follow-up study, the whole safety profile of a given medication can be obtained. Copyright © 2014 John Wiley & Sons, Ltd.

  15. Results of joint processing of data on nuclear and chemical explosions recorded on the long-range Quartz profile (Murmansk-Kyzyl)

    Science.gov (United States)

    Pavlenkova, G. A.; Pavlenkova, N. I.

    2008-04-01

    The long-range seismic profile Quartz, measured by the GEON Center (Ministry of Geology of the USSR), crosses a few large geostructures: the East European platform, Timan-Pechora plate, Northern Urals, West Siberian plate (WSP), and Altai. Observations of nuclear and chemical explosions were conducted on the profile. Joint processing of records from sources of both types provided detailed structures of the crust and upper mantle. They have confirmed the known patterns in the structure of these shells of the Earth and revealed new ones. Mountain roots are observed beneath the Urals and Altai, and areas of a higher heat flow are matched by lower velocity zones in the upper mantle. Moreover, it is shown that the Timan-Pechora plate is characterized by a two-layer crust untypical of other young plates of central Eurasia and the upper mantle has the same velocities beneath the ancient East European platform and the young Altai orogen. It is also shown that the vast region including the Timan-Pechora plate, Urals, and WSP is bounded on both sides by deep faults in the upper mantle dipping toward the center of Western Siberia. A few nearly continuous reflectors traceable in the upper mantle are represented by thin-layered heterogeneous beds. The largest horizontal heterogeneity is observed in the upper 100-km layer, often underlain by a lower velocity zone. The asthenosphere, as a layer of lower seismic velocities at the depth of a possible solidus (200 250 km), has not been revealed. The latter is evidently a feature specific to inner parts of the Eurasian continent; in marginal regions, e.g., in Western Europe, the asthenospheric layer is identified almost ubiquitously.

  16. Acute toxicity profile and compliance to accelerated radiotherapy plus carbogen and nicotinamide for clinical stage T2-4 laryngeal cancer: results of a phase III randomized trial.

    Science.gov (United States)

    Janssens, Geert O; Terhaard, Chris H; Doornaert, Patricia A; Bijl, Hendrik P; van den Ende, Piet; Chin, Alim; Pop, Lucas A; Kaanders, Johannes H

    2012-02-01

    To report the acute toxicity profile and compliance from a randomized Phase III trial comparing accelerated radiotherapy (AR) with accelerated radiotherapy plus carbogen and nicotinamide (ARCON) in laryngeal cancer. From April 2001 to February 2008, 345 patients with cT2-4 squamous cell laryngeal cancer were randomized to AR (n = 174) and ARCON (n = 171). Acute toxicity was scored weekly until Week 8 and every 2-4 weeks thereafter. Compliance to carbogen and nicotinamide was reported. Between both treatment arms (AR vs. ARCON) no statistically significant difference was observed for incidence of acute skin reactions (moist desquamation: 56% vs. 58%, p = 0.80), acute mucosal reactions (confluent mucositis: 79% vs. 85%, p = 0.14), and symptoms related to acute mucositis (severe pain on swallowing: 53% vs. 58%, p = 0.37; nasogastric tube feeding: 28% vs. 28%, p = 0.98; narcotic medicines required: 58% vs. 58%, p = 0.97). There was a statistically significant difference in median duration of confluent mucositis in favor of AR (2.0 vs 3.0 weeks, p = 0.01). There was full compliance with carbogen breathing and nicotinamide in 86% and 80% of the patients, with discontinuation in 6% and 12%, respectively. Adjustment of antiemesis prophylaxis was needed in 42% of patients. With the exception of a slight increase in median duration of acute confluent mucositis, the present data reveal a similar acute toxicity profile between both regimens and a good compliance with ARCON for clinical stage T2-4 laryngeal cancers. Treatment outcome and late morbidity will determine the real therapeutic benefit. Copyright © 2012 Elsevier Inc. All rights reserved.

  17. Energy spectra, angular spread, fluence profiles and dose distributions of 6 and 18 MV photon beams: results of Monte Carlo simulations for a Varian 2100EX accelerator

    Science.gov (United States)

    Ding, George X.

    2002-04-01

    The purpose of this study is to provide detailed characteristics of incident photon beams for different field sizes and beam energies. This information is critical to the future development of accurate treatment planning systems. It also enhances our knowledge of radiotherapy photon beams. The EGS4 Monte Carlo code, BEAM, has been used to simulate 6 and 18 MV photon beams from a Varian Clinac-2100EX accelerator. A simulated realistic beam is stored in a phase space data file, which contains details of each particle's complete history including where it has been and where it has interacted. The phase space files are analysed to obtain energy spectra, angular distribution, fluence profile and mean energy profiles at the phantom surface for particles separated according to their charge and history. The accuracy of a simulated beam is validated by the excellent agreement between the Monte Carlo calculated and measured dose distributions. Measured depth-dose curves are obtained from depth-ionization curves by accounting for newly introduced chamber fluence corrections and the stopping-power ratios for realistic beams. The study presents calculated depth-dose components from different particles as well as calculated surface dose and contribution from different particles to surface dose across the field. It is shown that the increase of surface dose with the increase of the field size is mainly due to the increase of incident contaminant charged particles. At 6 MV, the incident charged particles contribute 7% to 21% of maximum dose at the surface when the field size increases from 10 × 10 to 40 × 40 cm2. At 18 MV, their contributions are up to 11% and 29% of maximum dose at the surface for 10 × 10 cm2 and 40 × 40 cm2 fields respectively. However, the fluence of these incident charged particles is less than 1% of incident photon fluence in all cases.

  18. Sequential extraction results in improved proteome profiling of medicinal plant Pinellia ternata tubers, which contain large amounts of high-abundance proteins.

    Directory of Open Access Journals (Sweden)

    Xiaolin Wu

    Full Text Available Pinellia ternata tuber is one of the well-known Chinese traditional medicines. In order to understand the pharmacological properties of tuber proteins, it is necessary to perform proteome analysis of P. ternata tubers. However, a few high-abundance proteins (HAPs, mainly mannose-binding lectin (agglutinin, exist in aggregates of various sizes in the tubers and seriously interfere with proteome profiling by two-dimensional electrophoresis (2-DE. Therefore, selective depletion of these HAPs is a prerequisite for enhanced proteome analysis of P. ternata tubers. Based on differential protein solubility, we developed a novel protocol involving two sequential extractions for depletion of some HAPs and prefractionation of tuber proteins prior to 2-DE. The first extraction using 10% acetic acid selectively extracted acid-soluble HAPs and the second extraction using the SDS-containing buffer extracted remaining acid-insoluble proteins. After application of the protocol, 2-DE profiles of P. ternata tuber proteins were greatly improved and more protein spots were detected, especially low-abundance proteins. Moreover, the subunit composition of P. ternata lectin was analyzed by electrophoresis. Native lectin consists of two hydrogen-bonded subunits (11 kDa and 25 kDa and the 11 kDa subunit was a glycoprotein. Subsequently, major HAPs in the tubers were analyzed by mass spectrometry, with nine protein spots being identified as lectin isoforms. The methodology was easy to perform and required no specialized apparatus. It would be useful for proteome analysis of other tuber plants of Araceae.

  19. Data Profiling

    OpenAIRE

    Hladíková, Radka

    2010-01-01

    Title: Data Profiling Author: Radka Hladíková Department: Department of Software Engineering Supervisor: Ing. Vladimír Kyjonka Supervisor's e-mail address: Abstract: This thesis puts mind on problems with data quality and data profiling. This Work analyses and summarizes problems of data quality, data defects, process of data quality, data quality assessment and data profiling. The main topic is data profiling as a process of researching data available in existing...

  20. Show Me My Health Plans

    Directory of Open Access Journals (Sweden)

    Mary C. Politi PhD

    2016-11-01

    Full Text Available Introduction: Since the Affordable Care Act was passed, more than 12 million individuals have enrolled in the health insurance marketplace. Without support, many struggle to make an informed plan choice that meets their health and financial needs. Methods: We designed and evaluated a decision aid, Show Me My Health Plans (SMHP, that provides education, preference assessment, and an annual out-of-pocket cost calculator with plan recommendations produced by a tailored, risk-adjusted algorithm incorporating age, gender, and health status. We evaluated whether SMHP compared to HealthCare.gov improved health insurance decision quality and the match between plan choice, needs, and preferences among 328 Missourians enrolling in the marketplace. Results: Participants who used SMHP had higher health insurance knowledge (LS-Mean = 78 vs. 62; P < 0.001, decision self-efficacy (LS-Mean = 83 vs. 75; P < 0.002, confidence in their choice (LS-Mean = 3.5 vs. 2.9; P < 0.001, and improved health insurance literacy (odds ratio = 2.52, P < 0.001 compared to participants using HealthCare.gov . Those using SMHP were 10.3 times more likely to select a silver- or gold-tier plan (P < 0.0001. Discussion: SMHP can improve health insurance decision quality and the odds that consumers select an insurance plan with coverage likely needed to meet their health needs. This study represents a unique context through which to apply principles of decision support to improve health insurance choices.

  1. Facial profile esthetic preferences: perception in two Brazilian states

    Directory of Open Access Journals (Sweden)

    Marina Detoni Vieira de Oliveira

    2015-06-01

    Full Text Available OBJECTIVE: The aim of this study was to assess the regional influence on the perception of facial profile esthetics in Rio de Janeiro state (RJ and Rio Grande do Sul state (RS, Brazil. METHODS: Two Caucasian models, a man and a woman, with balanced facial profiles, had their photographs digitally manipulated so as to produce seven different profiles. First year dental students (laypeople assessed the images and classified them according to their esthetic preference. RESULTS: The result of the t test for independent samples showed differences among states for certain facial profiles. The female photograph identified with the letter 'G' (mandibular retrusion received higher scores in RS state (p = 0.006. No differences were found for male photographs (p > 0.007. The evaluators' sex seemed not to influence their esthetic perception (p > 0.007. Considering all evaluators together, ANOVA/Tukey's test showed differences among the profiles (p ≤ 0.05 for both male and female photographs. The female photograph that received the highest score was the one identified with the letter 'F' (dentoalveolar bimaxillary retrusion/ straight profile. For the male profiles, photograph identified with the letter 'E' (dentoalveolar bimaxillary protrusion/ straight profile received the best score. CONCLUSION: Regional differences were observed regarding preferences of facial profile esthetics. In Rio de Janeiro state, more prominent lips were preferred while in Rio Grande do Sul state, profiles with straight lips were favored. Class III profiles were considered less attractive.

  2. Hey Teacher, Your Personality's Showing!

    Science.gov (United States)

    Paulsen, James R.

    1977-01-01

    A study of 30 fourth, fifth, and sixth grade teachers and 300 of their students showed that a teacher's age, sex, and years of experience did not relate to students' mathematics achievement, but that more effective teachers showed greater "freedom from defensive behavior" than did less effective teachers. (DT)

  3. Planning a Successful Tech Show

    Science.gov (United States)

    Nikirk, Martin

    2011-01-01

    Tech shows are a great way to introduce prospective students, parents, and local business and industry to a technology and engineering or career and technical education program. In addition to showcasing instructional programs, a tech show allows students to demonstrate their professionalism and skills, practice public presentations, and interact…

  4. Prevalence and cardiovascular risk profile of chronic kidney disease in Italy: results of the 2008-12 National Health Examination Survey.

    Science.gov (United States)

    De Nicola, Luca; Donfrancesco, Chiara; Minutolo, Roberto; Lo Noce, Cinzia; Palmieri, Luigi; De Curtis, Amalia; Iacoviello, Licia; Zoccali, Carmine; Gesualdo, Loreto; Conte, Giuseppe; Vanuzzo, Diego; Giampaoli, Simona

    2015-05-01

    National surveys in countries outside Europe have reported a high prevalence (11-13%) of chronic kidney disease (CKD). Studies in Europe have provided a variable prevalence likely due to differences in study design, including age and extent of geographic areas, equation used to evaluate estimated glomerular filtration rate (eGFR) and CKD stages examined. The 2008-12 National Health Examination Survey in Italy randomly extracted samples from the general population aged 35-79 years, stratified by age and gender, from the resident list of each Italian region (440 persons/1.5 million of residents). We estimated the prevalence of CKD by means of urinary albumin : creatinine ratio and eGFR (CKD-EPI equation-enzymatic assay of serum creatinine). Cardiovascular (CV) risk profile was also evaluated. Three thousand eight hundred and forty-eight men and 3704 women were examined. In the whole population, mean age was 57 ± 12 and 56 ± 12 years in men and women, respectively; hypertension was prevalent in men and women, respectively (56 and 43%) and the same held true for overweight (48 and 33%), obesity (26 and 27%), diabetes (14 and 9%) and smoking (21 and 18%), whereas CV disease was less frequent (9 and 6%). Overall, the prevalence of CKD (95% confidence interval) was 7.05% (6.48-7.65). Early stages constituted 59% of the CKD population [Stage G1-2 A2-3: 4.16% (3.71-4.61) and Stage G3-5: 2.89% (2.51-3.26)]. At multivariate regression analysis, age, obesity, hypertension, diabetes, CV disease and smoking were all independent correlates of CKD. CKD has a relatively lower prevalence in Italy, in particular for advanced stages, when compared with similar national surveys outside Europe. This occurs despite older age and unfavourable CV risk profile of the whole population. © The Author 2014. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved.

  5. Dependence of Selected Water Quality Parameters on Flow Rates in River Profiles in the Czech Republic

    Directory of Open Access Journals (Sweden)

    Eduard Hanslík

    2016-06-01

    The results show that in the monitored profiles, there is a direct relationship with flow rate in case of N-NO3-, suspended solids and O2. Temperature shows an inverse relationship with the flow rate. Other parameters show different relationship with the flow rate in individual monitored profiles or do not show statistically significant relation.

  6. Socio-demographic and reproductive health profile of women who experienced signs of obstetric fistula: Results from Pakistan Demographic and Health Survey (PDHS) 2006-2007.

    Science.gov (United States)

    Khan, Sadaf; Zaheer, Sidra

    2017-11-01

    to examine the socio-demographic profile of women experiencing signs of obstetric fistula and factors contributing to the development of this condition in Pakistan. secondary data analysis of Pakistan Demographic and Health Survey 2006-07, which for the first time measured signs of obstetric fistula, using a questionnaire at a population level. For the present study, questions directly related to obstetric fistula signs were used to construct a dependent variable. Data were analysed by descriptive and logistic regression analysis, to examine factors associated with development of fistula. among women of reproductive age (n = 9134, aged 15-49 years), some 277 (3.0%, 30 per 1000 women who ever gave birth) experienced obstetric fistula signs, whereas 103 (1.1% of all women) were still experiencing the condition at the time of survey. In a logistic regression model, women from the Punjab region (OR = 5.67, 95%CI = 2.10-15.31), women who delivered by caesarean section (OR = 1.91, 95% CI = 1.06-3.42) and reported having complications during pregnancy were more likely to develop obstetric fistula (OR = 1.96, 95%CI = 1.19-3.16). Obstetric fistula is one of the neglected public and reproductive health concerns in Pakistan. To eliminate this preventable tragedy, there is a need for better emergency obstetric care facilities and the availability of a fistula repair service throughout the country. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Risk Aversion in Game Shows

    DEFF Research Database (Denmark)

    Andersen, Steffen; Harrison, Glenn W.; Lau, Morten I.

    2008-01-01

    We review the use of behavior from television game shows to infer risk attitudes. These shows provide evidence when contestants are making decisions over very large stakes, and in a replicated, structured way. Inferences are generally confounded by the subjective assessment of skill in some games......, and the dynamic nature of the task in most games. We consider the game shows Card Sharks, Jeopardy!, Lingo, and finally Deal Or No Deal. We provide a detailed case study of the analyses of Deal Or No Deal, since it is suitable for inference about risk attitudes and has attracted considerable attention....

  8. Measuring performance at trade shows

    DEFF Research Database (Denmark)

    Hansen, Kåre

    2004-01-01

    Trade shows is an increasingly important marketing activity to many companies, but current measures of trade show performance do not adequately capture dimensions important to exhibitors. Based on the marketing literature's outcome and behavior-based control system taxonomy, a model is built...... that captures a outcome-based sales dimension and four behavior-based dimensions (i.e. information-gathering, relationship building, image building, and motivation activities). A 16-item instrument is developed for assessing exhibitors perceptions of their trade show performance. The paper presents evidence...... of the scale's reliability, factor structure, and validity on the basis of analyzing data from independent samples of exhibitors at the international trade shows SIAL (Paris) and ANUGA (Cologne); and it concludes with a discussion of potential managerial applications and implications for future research. New...

  9. Yoga based cardiac rehabilitation after coronary artery bypass surgery: One-year results on LVEF, lipid profile and psychological states – A randomized controlled study

    Science.gov (United States)

    Raghuram, Nagarathna; Parachuri, Venkateshwara Rao; Swarnagowri, M.V.; Babu, Suresh; Chaku, Ritu; Kulkarni, Ravi; Bhuyan, Bhagavan; Bhargav, Hemant; Nagendra, Hongasandra Ramarao

    2014-01-01

    Objective To compare the long term effects of yoga based cardiac rehabilitation program with only physiotherapy based program as an add-on to conventional rehabilitation after coronary artery bypass grafting (CABG) on risk factors. Methods In this single blind prospective randomized parallel two armed active control study, 1026 patients posted for CABG at Narayana Hrudayalaya Institute of Cardiac Sciences, Bengaluru (India) were screened. Of these, 250 male participants (35–65 years) who satisfied the selection criteria and consented were randomized into two groups. Within and between group comparisons were done at three points of follow up (i.e. 6th week, 6th month, and 12th month) by using Wilcoxon's signed ranks test and Mann Whitney U test respectively. Results Yoga group had significantly (p = 0.001, Mann Whitney) better improvement in LVEF than control group in those with abnormal baseline EF (yoga group (p = 0.038, between groups) in those with high baseline BMI (≥23) after 12 months. Yoga group showed significant (p = 0.008, Wilcoxon's) reduction in blood glucose at one year in those with high baseline FBS ≥110 mg/dl. There was significantly better improvement in yoga than the control group in HDL (p = 0.003), LDL (p = 0.01) and VLDL (p = 0.03) in those with abnormal baseline values. There was significantly better improvement (p = 0.02, between groups) in positive affect in yoga group. Within Yoga group, there was significant decrease in perceived stress (p = 0.001), anxiety (p = 0.001), depression (p = 0.001), and negative affect (p = 0.03) while in the control group there was reduction (p = 0.003) only in scores on anxiety. Conclusion Addition of yoga based relaxation to conventional post-CABG cardiac rehabilitation helps in better management of risk factors in those with abnormal baseline values and may help in preventing recurrence. PMID:25443601

  10. Gender and age differences in the perception of bother and health care seeking for lower urinary tract symptoms: results from the hospitalised and outpatients' profile and expectations study.

    Science.gov (United States)

    Apostolidis, Apostolos; Kirana, Paraskevi-Sofia; Chiu, Gretchen; Link, Carol; Tsiouprou, Marina; Hatzichristou, Dimitrios

    2009-12-01

    Few comparisons have been made of health care seeking behaviour for lower urinary tract symptoms (LUTS) between men and women, as well as trends across age groups. To investigate the bother from LUTS and effect on health care seeking in both men and women of different age groups and in comparison between the two genders. A representative cross section of each of 13 clinics of a general academic hospital, with equal numbers of subjects recruited in each of six design cells that were defined by age (18-40, 41-60, 61-80 yr) and gender. A 2-h in-person interview, conducted by a trained psychologist/interviewer in a clinic office. Severity of LUTS was measured by the International Prostate Symptom Score (IPSS). Treatment seeking was measured by a single item. A bother question was modified to assess overall bother. Impact on quality of life (QoL) was measured by the IPSS QoL question. The final study sample comprised 415 patients. More women than men reported the presence of LUTS (85.5% vs 75.2%; p=0.01). LUTS were more bothersome in women (25.4% of women vs 17.6% of men with bother "some" or "a lot"; p=0.02). Severity of LUTS increased with age in both genders (men: pgenders (ponly in men (ponly bother from LUTS was associated with treatment seeking in women, compared with bother, age, and the presence of voiding symptoms in men. In our hospital-based sample, differences in LUTS frequency, bother, and health care seeking profiles between men and women suggest a different perception and response to LUTS between the two genders.

  11. A note on critical dimensions in profile semiparametric estimation

    OpenAIRE

    Andresen, Andreas

    2014-01-01

    This paper complements the results of Andresen et. al "Critical dimension in profile semiparametric estimation" (2014) on profile estimators in semiparametric models. We present two examples. One that illustrates that the smoothness constraint on the expected value of the contrast functional used to define the profile M-estimator is necessary for the bound derived for the critical ratio of dimension to sample size. A second one to show that in the case that the target dimension is proportiona...

  12. Tokyo Motor Show 2003; Tokyo Motor Show 2003

    Energy Technology Data Exchange (ETDEWEB)

    Joly, E.

    2004-01-01

    The text which follows present the different techniques exposed during the 37. Tokyo Motor Show. The report points out the great tendencies of developments of the Japanese automobile industry. The hybrid electric-powered vehicles or those equipped with fuel cells have been highlighted by the Japanese manufacturers which allow considerable budgets in the research of less polluting vehicles. The exposed models, although being all different according to the manufacturer, use always a hybrid system: fuel cell/battery. The manufacturers have stressed too on the intelligent systems for navigation and safety as well as on the design and comfort. (O.M.)

  13. Reality show: um paradoxo nietzschiano

    Directory of Open Access Journals (Sweden)

    Ilana Feldman

    2011-01-01

    Full Text Available

    O fenômeno dos reality shows - e a subseqüente relação entre imagem e verdade - assenta-se sobre uma série de paradoxos. Tais paradoxos podem ser compreendidos à luz do pensamento do filósofo alemão Friedrich Nietzsche, que, através dos usos de formulações paradoxais, concebia a realidade como um mundo de pura aparência e a verdade como um acréscimo ficcional, como um efeito. A ficção é então tomada, na filosofia de Nietzsche, não em seu aspecto falsificante e desrealizador - como sempre pleiteou nossa tradição metafísica -, mas como condição necessária para que certa espécie de invenção possa operar como verdade. Sendo assim, a própria expressão reality show, através de sua formulação paradoxal, engendra explicitamente um mundo de pura aparência, em que a verdade, a parte reality da proposição, é da ordem do suplemento, daquilo que se acrescenta ficcionalmente - como um adjetivo - a show. O ornamento, nesse caso, passa a ocupar o lugar central, apontando para o efeito produzido: o efeito-de-verdade. Seguindo, então, o pensamento nietzschiano e sua atualização na contemporaneidade, investigaremos de que forma os televisivos “shows de realidade” operam paradoxalmente, em consonância com nossas paradoxais práticas culturais.

  14. LIPID PROFILE IN SUDDEN SENSORINEURAL HEARING LOSS- A PROSPECTIVE STUDY

    National Research Council Canada - National Science Library

    Anil H. T; S. D. Mahamood Pasha

    2017-01-01

    .... Relevant history was obtained. Examination and investigations were conducted. RESULTS Statistical analysis showed that there was significant difference between the means of lipid profile of the patients and the control group...

  15. Profils d’apprentissage et représentations dans l’apprentissage des langues en environnement multimédia. Résultats d’une enquête en contexte universitaire. Learner profiles and attitudes in foreign language learning in a multimedia environment. Results of a survey conducted in a higher education context.

    Directory of Open Access Journals (Sweden)

    Joseph Rézeau

    1999-06-01

    Full Text Available Dans le domaine de l’apprentissage, les profils d’apprentissage et les représentations des apprenants jouent un rôle fondamental. La recherche exposée dans cet article met en évidence des différences sensibles de profil d’apprentissage des langues chez des étudiants spécialisés en langues d’une part et des étudiants d’autres disciplines d’autre part. Elle montre également que les représentations de l’apprentissage des langues et celles des Technologies de l’Information et de la Communication (TIC chez ces deux populations sont des représentations sociales, qui montrent un recouvrement entre les deux domaines étudiés. Enfin, une étude longitudinale d’un échantillon de la population étudiée montre que les profils d’apprentissage et les représentations sont susceptibles d’évolution. La question reste posée de l’influence de l’environnement multimédia d’apprentissage de la langue sur cette évolution constatée.In the field of learning, the learners’ attitudes and representations play a fundamental role. The research presented in this article shows significant differences in the language learning profiles of university students specialised in languages on the one hand and specialists of other disciplines on the other hand. Our research also shows that the representations of language learning and the representations of the Information and Communication Technologies (ICT held by the subjects in these two populations are social representations, showing overlap between the two domains studied. Finally, a longitudinal study of a sample of the population under review shows that both learners’ profiles and representations are open to evolution. The question remains of the influence exerted by the multimedia language learning environment over the said evolution.

  16. Modeling of Changing Electrode Profiles

    Energy Technology Data Exchange (ETDEWEB)

    Prentice, Geoffrey Allen [Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials and Molecular Research Division; Univ. of California, Berkeley, CA (United States). Dept. of Chemical Engineering

    1980-12-01

    A model for simulating the transient behavior of solid electrodes undergoing deposition or dissolution has been developed. The model accounts for ohmic drop, charge transfer overpotential, and mass transport limitations. The finite difference method, coupled with successive overrelaxation, was used as the basis of the solution technique. An algorithm was devised to overcome the computational instabilities associated with the calculations of the secondary and tertiary current distributions. Simulations were performed on several model electrode profiles: the sinusoid, the rounded corner, and the notch. Quantitative copper deposition data were obtained in a contoured rotating cylinder system, Sinusoidal cross-sections, machined on stainless steel cylinders, were used as model geometries, Kinetic parameters for use in the simulation were determined from polarization curves obtained on copper rotating cylinders, These parameters, along with other physical property and geometric data, were incorporated in simulations of growing sinusoidal profiles. The copper distributions on the sinusoidal cross-sections were measured and found to compare favorably with the simulated results. At low Wagner numbers the formation of a slight depression at the profile peak was predicted by the simulation and observed on the profile. At higher Wagner numbers, the simulated and experimental results showed that the formation of a depression was suppressed. This phenomenon was shown to result from the competition between ohmic drop and electrode curvature.

  17. Graphene Oxides Show Angiogenic Properties.

    Science.gov (United States)

    Mukherjee, Sudip; Sriram, Pavithra; Barui, Ayan Kumar; Nethi, Susheel Kumar; Veeriah, Vimal; Chatterjee, Suvro; Suresh, Kattimuttathu Ittara; Patra, Chitta Ranjan

    2015-08-05

    Angiogenesis, a process resulting in the formation of new capillaries from the pre-existing vasculature plays vital role for the development of therapeutic approaches for cancer, atherosclerosis, wound healing, and cardiovascular diseases. In this report, the synthesis, characterization, and angiogenic properties of graphene oxide (GO) and reduced graphene oxide (rGO) have been demonstrated, observed through several in vitro and in vivo angiogenesis assays. The results here demonstrate that the intracellular formation of reactive oxygen species and reactive nitrogen species as well as activation of phospho-eNOS and phospho-Akt might be the plausible mechanisms for GO and rGO induced angiogenesis. The results altogether suggest the possibilities for the development of alternative angiogenic therapeutic approach for the treatment of cardiovascular related diseases where angiogenesis plays a significant role. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. YOUNG ATHLETES' MOTIVATIONAL PROFILES

    Directory of Open Access Journals (Sweden)

    Juan Antonio Moreno Murcia

    2007-06-01

    Full Text Available The aim of this study was to examine the relationship between motivational characteristics and dispositional flow. In order to accomplish this goal, motivational profiles emerging from key constructs within Achievement Goal Theory and Self-Determination Theory were related to the dispositional flow measures. A sample of 413 young athletes (Age range 12 to 16 years completed the PMCSQ-2, POSQ, SMS and DFS measures. Cluster analysis results revealed three profiles: a "self-determined profile" characterised by higher scores on the task-involving climate perception and on the task orientation; a "non-self-determined profile", characterised by higher scores on ego-involving climate perception and ego orientation; and a "low self-determined and low non-self-determined profile" which had the lowest dispositional flow. No meaningful differences were found between the "self-determined profile" and the "non-self-determined profile" in dispositional flow. The "self-determined profile" was more commonly associated with females, athletes practising individual sports and those training more than three days a week. The "non-self-determined profile" was more customary of males and athletes practising team sports as well as those training just two or three days a week

  19. What Do Blood Tests Show?

    Science.gov (United States)

    ... 9 to 12 hours of fasting. High blood cholesterol is a risk factor for coronary heart disease . Your doctor should discuss your results ... NEWS August 04, 2015 | News Release Measurement of Cholesterol Function Might Provide Link to Heart Attack Risk in Patients with Psoriasis WHAT: Scientists now report ...

  20. Nutritional Stress Induced by Tryptophan-Degrading Enzymes Results in ATF4-Dependent Reprogramming of the Amino Acid Transporter Profile in Tumor Cells

    DEFF Research Database (Denmark)

    Timosenko, Elina; Ghadbane, Hemza; Silk, Jonathan D

    2016-01-01

    Tryptophan degradation is an immune escape strategy shared by many tumors. However, cancer cells' compensatory mechanisms remain unclear. We demonstrate here that a shortage of tryptophan caused by expression of indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO) resulted in AT...

  1. Use of metabolic profiling to study grape skin polyphenol behavior as a result of canopy microclimate manipulation in a 'Pinot noir' vineyard.

    Science.gov (United States)

    Sternad Lemut, Melita; Sivilotti, Paolo; Franceschi, Pietro; Wehrens, Ron; Vrhovsek, Urska

    2013-09-18

    Canopy microclimate manipulation can have a significant effect on grapevine gene expression and can thus affect the yield of many important berry compounds. Focusing on only a few targeted phenolics in the past, advanced multimethod analytical approaches are opening up much wider possibilities to fill in the gaps of missing knowledge about plant secondary metabolism. Different leaf removal timings, leading to different microclimate scenarios, were thus introduced in a 'Pinot noir' vineyard to reveal related alterations of multiple classes of skin phenolics, including some rarely studied to date. Different accumulation trends during cluster development were detected not only between groups but also between individual compounds within groups. Although many significant changes were observed early in the season, these were later often less significant. However, at harvest, 31 of 72 detected compounds showed significant differences in comparison to control for at least one of three leaf removal approaches.

  2. Nutritional Stress Induced by Tryptophan-Degrading Enzymes Results in ATF4-Dependent Reprogramming of the Amino Acid Transporter Profile in Tumor Cells.

    Science.gov (United States)

    Timosenko, Elina; Ghadbane, Hemza; Silk, Jonathan D; Shepherd, Dawn; Gileadi, Uzi; Howson, Lauren J; Laynes, Robert; Zhao, Qi; Strausberg, Robert L; Olsen, Lars R; Taylor, Stephen; Buffa, Francesca M; Boyd, Richard; Cerundolo, Vincenzo

    2016-11-01

    Tryptophan degradation is an immune escape strategy shared by many tumors. However, cancer cells' compensatory mechanisms remain unclear. We demonstrate here that a shortage of tryptophan caused by expression of indoleamine 2,3-dioxygenase (IDO) and tryptophan 2,3-dioxygenase (TDO) resulted in ATF4-dependent upregulation of several amino acid transporters, including SLC1A5 and its truncated isoforms, which in turn enhanced tryptophan and glutamine uptake. Importantly, SLC1A5 failed to be upregulated in resting human T cells kept under low tryptophan conditions but was enhanced upon cognate antigen T-cell receptor engagement. Our results highlight key differences in the ability of tumor and T cells to adapt to tryptophan starvation and provide important insights into the poor prognosis of tumors coexpressing IDO and SLC1A5. Cancer Res; 76(21); 6193-204. ©2016 AACR. ©2016 American Association for Cancer Research.

  3. A ranking index for quality assessment of forensic DNA profiles forensic DNA profiles

    Directory of Open Access Journals (Sweden)

    Ansell Ricky

    2010-11-01

    Full Text Available Abstract Background Assessment of DNA profile quality is vital in forensic DNA analysis, both in order to determine the evidentiary value of DNA results and to compare the performance of different DNA analysis protocols. Generally the quality assessment is performed through manual examination of the DNA profiles based on empirical knowledge, or by comparing the intensities (allelic peak heights of the capillary electrophoresis electropherograms. Results We recently developed a ranking index for unbiased and quantitative quality assessment of forensic DNA profiles, the forensic DNA profile index (FI (Hedman et al. Improved forensic DNA analysis through the use of alternative DNA polymerases and statistical modeling of DNA profiles, Biotechniques 47 (2009 951-958. FI uses electropherogram data to combine the intensities of the allelic peaks with the balances within and between loci, using Principal Components Analysis. Here we present the construction of FI. We explain the mathematical and statistical methodologies used and present details about the applied data reduction method. Thereby we show how to adapt the ranking index for any Short Tandem Repeat-based forensic DNA typing system through validation against a manual grading scale and calibration against a specific set of DNA profiles. Conclusions The developed tool provides unbiased quality assessment of forensic DNA profiles. It can be applied for any DNA profiling system based on Short Tandem Repeat markers. Apart from crime related DNA analysis, FI can therefore be used as a quality tool in paternal or familial testing as well as in disaster victim identification.

  4. A ranking index for quality assessment of forensic DNA profiles forensic DNA profiles

    Science.gov (United States)

    2010-01-01

    Background Assessment of DNA profile quality is vital in forensic DNA analysis, both in order to determine the evidentiary value of DNA results and to compare the performance of different DNA analysis protocols. Generally the quality assessment is performed through manual examination of the DNA profiles based on empirical knowledge, or by comparing the intensities (allelic peak heights) of the capillary electrophoresis electropherograms. Results We recently developed a ranking index for unbiased and quantitative quality assessment of forensic DNA profiles, the forensic DNA profile index (FI) (Hedman et al. Improved forensic DNA analysis through the use of alternative DNA polymerases and statistical modeling of DNA profiles, Biotechniques 47 (2009) 951-958). FI uses electropherogram data to combine the intensities of the allelic peaks with the balances within and between loci, using Principal Components Analysis. Here we present the construction of FI. We explain the mathematical and statistical methodologies used and present details about the applied data reduction method. Thereby we show how to adapt the ranking index for any Short Tandem Repeat-based forensic DNA typing system through validation against a manual grading scale and calibration against a specific set of DNA profiles. Conclusions The developed tool provides unbiased quality assessment of forensic DNA profiles. It can be applied for any DNA profiling system based on Short Tandem Repeat markers. Apart from crime related DNA analysis, FI can therefore be used as a quality tool in paternal or familial testing as well as in disaster victim identification. PMID:21062433

  5. Immortalization of T-Cells Is Accompanied by Gradual Changes in CpG Methylation Resulting in a Profile Resembling a Subset of T-Cell Leukemias

    Directory of Open Access Journals (Sweden)

    Sofie Degerman

    2014-07-01

    Full Text Available We have previously described gene expression changes during spontaneous immortalization of T-cells, thereby identifying cellular processes important for cell growth crisis escape and unlimited proliferation. Here, we analyze the same model to investigate the role of genome-wide methylation in the immortalization process at different time points pre-crisis and post-crisis using high-resolution arrays. We show that over time in culture there is an overall accumulation of methylation alterations, with preferential increased methylation close to transcription start sites (TSSs, islands, and shore regions. Methylation and gene expression alterations did not correlate for the majority of genes, but for the fraction that correlated, gain of methylation close to TSS was associated with decreased gene expression. Interestingly, the pattern of CpG site methylation observed in immortal T-cell cultures was similar to clinical T-cell acute lymphoblastic leukemia (T-ALL samples classified as CpG island methylator phenotype positive. These sites were highly overrepresented by polycomb target genes and involved in developmental, cell adhesion, and cell signaling processes. The presence of non-random methylation events in in vitro immortalized T-cell cultures and diagnostic T-ALL samples indicates altered methylation of CpG sites with a possible role in malignant hematopoiesis.

  6. Beach Profile Locations

    Data.gov (United States)

    California Department of Resources — Beaches are commonly characterized by cross-shore surveys. The resulting profiles represent the elevation of the beach surface and nearshore seabed from the back of...

  7. Grustal shortening in the Alpine Orogen: Results from deep seismic reflection profiling in the eastern Swiss Alps, Line NFP 20-east

    Science.gov (United States)

    Pfiffner, O. A.; Frei, W.; Valasek, P.; StäUble, M.; Levato, L.; Dubois, L.; Schmid, S. M.; Smithson, S. B.

    1990-12-01

    The deep crustal seismic line NFP 20-EAST crosses almost the entire Swiss Alps. Despite the complex geometry of the well-exposed nappe structure and the considerable axial plunge of some of the units, the Vibroseis survey yielded coherent reflections from several individually identifiable nappe contacts. In the northern part of the survey the Vibroseis data closely match the internal structure of the Helvetic nappes and the underlying autochthonous-parautochthonous Mesozoic sediments. On the northern flank of the Aar massif, an external basement uplift, these Mesozoic sediments seem to rise from a depth of approximately 7-8 km below sea level to the surface in a series of steps which is interpreted to represent crustal shortening achieved by a combination of folding and thrusting. In the southern part of the survey it was possible to image a number of thin slivers of Mesozoic carbonates pinched between slabs of Penninic basement nappes as well as nappe contacts between lithologically contrasting units. In addition, it seems that the Insubric fault zone, which marks the contact between the Penninic zone and the Southern Alps and which outcrops about 30 km to the south of the survey, shows up as steeply north dipping reflections. The lower European crust in the northern part of the survey is relatively transparent as opposed to the Adriatic lower crust, whose reflective nature may stem from shear zones associated with Mesozoic crustal stretching. The base of both the European and Adriatic crust coincides with a 1-s-thick band of laterally discontinuous reflections. This reflection Moho drops to greater depths going from the north toward the center of the Alpine chain, where it disappears with a steep southerly dip. The Moho reappears as a reflection band farther south. This Moho gap is situated above the lithospheric root and may be caused by perturbations related to subduction of lower crustal material. The crustal-scale structure obtained from the Vibroseis data

  8. Symptoms of Anxiety and Depression Are Associated With Satisfaction With Information Provision and Internet Use Among 3080 Cancer Survivors: Results of the PROFILES Registry.

    Science.gov (United States)

    Beekers, Nienke; Husson, Olga; Mols, Floortje; van Eenbergen, Mies; van de Poll-Franse, Lonneke V

    2015-01-01

    Dissatisfaction with information provided by healthcare providers may be a reason for cancer survivors to seek health information on the Internet and may also result in more symptoms of anxiety and depression among this population. The aim of this study was to investigate whether anxious and depressive symptoms are associated with satisfaction with information provision and Internet use among cancer survivors. All individuals diagnosed with endometrial or colorectal cancer between 1998 and 2007 or lymphoma or multiple myeloma between 1999 and 2008 as registered in the Eindhoven Cancer Registry were invited for participation. In total, 4 446 survivors received a questionnaire including the 25-item European Organisation for Research and Treatment of Cancer Quality of Life Group Information questionnaire and Hospital Anxiety and Depression Scale; 69% responded (n = 3080). Having anxious (odds ratio [OR], 0.7; 95% confidence interval [CI], 0.5-0.9; P information. Having depressive symptoms or having both depressive and anxious symptoms were negatively associated with satisfaction with information (OR, 0.5; 95% CI, 0.4-0.7; OR, 0.5; 95% CI, 0.4-0.7, respectively; P information provision is suboptimal, either because it is not adjusted to the mental health status of cancer patients or because it is unsatisfactory and thereby causing anxious and depressive symptoms among cancer patients. More attention should be paid to optimally adjust the information provision to the individual needs of the cancer patients for better mental health.

  9. Biophysical Profile

    Science.gov (United States)

    ... and pregnancy High-risk pregnancy Biophysical profile About Advertisement Mayo Clinic does not endorse companies or products. ... a Job Site Map About This Site Twitter Facebook Google YouTube Pinterest Mayo Clinic is a not- ...

  10. Hanford Site Ecological Quality Profile

    Energy Technology Data Exchange (ETDEWEB)

    Bilyard, Gordon R.; Sackschewsky, Michael R.; Tzemos, Spyridon

    2002-02-17

    This report reviews the ecological quality profile methodology and results for the Hanford Site. It covers critical ecological assets and terrestrial resources, those in Columbia River corridor and those threatened and engdangered, as well as hazards and risks to terrestrial resources. The features of a base habitat value profile are explained, as are hazard and ecological quality profiles.

  11. The Comparison Between Chinese Puppet Show and Chinese-Javanese Blend Puppet Show in Indonesia

    Directory of Open Access Journals (Sweden)

    Agustinus Sufianto

    2011-04-01

    Full Text Available Since some centuries ago, Indonesia has been one of the destinations for immigrating Chinese. When the Chinese came to Indonesia, they also brought their cultures and customs. Some of them have a cultural-cross marriage with local Indonesian and brought their cultures to be introduced to local Indonesian. When the relationship between Chinese and Indonesian became stronger in some fields such as trade, education and politic, some of Chinese cultures has been adapted by local Indonesian or has been assimilated with local cultures. This adaption process was hindered when the New Order of Soeharto government had banned all Chinese culture, including anything closely related. So, the Chinese-Javanese blend puppet show that also brings Chinese culture couldn’t get any supports and its development faces difficulties. The writer using a qualitative methodology interviewed some of Chinese-Javanese puppet show artist and observation of the puppet show performance to make a comparison between Chinese puppet show and Chinese-Javanese blend puppet show in Indonesia. Chinese-Javanese puppet show or usually can be determined as Indonesian potehi puppet Show has some differences from Chinese puppet show. The differences can be found in the making of puppet, puppet profiles, performance way and stories. 

  12. A ranking index for quality assessment of forensic DNA profiles forensic DNA profiles.

    Science.gov (United States)

    Hedman, Johannes; Ansell, Ricky; Nordgaard, Anders

    2010-11-09

    Assessment of DNA profile quality is vital in forensic DNA analysis, both in order to determine the evidentiary value of DNA results and to compare the performance of different DNA analysis protocols. Generally the quality assessment is performed through manual examination of the DNA profiles based on empirical knowledge, or by comparing the intensities (allelic peak heights) of the capillary electrophoresis electropherograms. We recently developed a ranking index for unbiased and quantitative quality assessment of forensic DNA profiles, the forensic DNA profile index (FI) (Hedman et al. Improved forensic DNA analysis through the use of alternative DNA polymerases and statistical modeling of DNA profiles, Biotechniques 47 (2009) 951-958). FI uses electropherogram data to combine the intensities of the allelic peaks with the balances within and between loci, using Principal Components Analysis. Here we present the construction of FI. We explain the mathematical and statistical methodologies used and present details about the applied data reduction method. Thereby we show how to adapt the ranking index for any Short Tandem Repeat-based forensic DNA typing system through validation against a manual grading scale and calibration against a specific set of DNA profiles. The developed tool provides unbiased quality assessment of forensic DNA profiles. It can be applied for any DNA profiling system based on Short Tandem Repeat markers. Apart from crime related DNA analysis, FI can therefore be used as a quality tool in paternal or familial testing as well as in disaster victim identification.

  13. The Dark Side of the Affective Profiles

    Directory of Open Access Journals (Sweden)

    Danilo Garcia

    2015-12-01

    Full Text Available The affective profiles model is based on the combination of individuals’ experience of high/low positive affect and high/low negative affect: self-fulfilling, high affective, low affective, and self-destructive. We used the profiles as the backdrop for the investigation of individual differences in malevolent character traits (i.e., the Dark Triad: psychopathy, Machiavellianism, and narcissism. A total of 1,000 participants (age: M = 31.50 SD = 10.27, 667 males and 333 females, recruited through Amazons’ Mechanical Turk (MTurk, responded to the Positive Affect Negative Affect Schedule and the Dark Triad Dirty Dozen. Individuals with a high affective profile reported higher degree of narcissism than those with any other profile, and together with individuals with a self-destructive profile, also higher degree of Machiavellianism and psychopathy than individuals with a low affective and self-fulfilling profile. Males scored higher in Machiavellianism and psychopathy. Together with earlier findings, our results show that while individuals in both the self-fulfilling and high affective profiles are extrovert and self-directed, only those in the high affective profile express an immature and malevolent character (i.e., high levels of all Dark Triad traits. Conversely, individuals in the self-fulfilling profile have earlier reported higher levels of cooperativeness and faith. More importantly, the unique association between high levels of positive emotions and narcissism and the unified association between negative emotions to both psychopathy and Machiavellianism imply a dyad rather than a triad of malevolent character traits.

  14. Association between gene expression profiles and clinical outcome of pemetrexed-based treatment in patients with advanced non-squamous non-small cell lung cancer: exploratory results from a phase II study.

    Directory of Open Access Journals (Sweden)

    Dean A Fennell

    Full Text Available We report exploratory gene-expression profiling data from a single-arm Phase-II-study in patients with non-squamous (nsNSCLC treated with pemetrexed and cisplatin. Previously disclosed results indicated a significant association of low thymidylate-synthase (TS-expression with longer progression-free and overall survival (PFS/OS.Treatment-naïve nsNSCLC patients (IIIB/IV received 4 cycles of pemetrexed/cisplatin; non-progressing patients continued on pemetrexed-maintenance. Diagnostic tissue-samples were used to assess TS-expression by immunohistochemistry (IHC and mRNA-expression array-profiling (1,030 lung cancer-specific genes. Cox proportional-hazard models were applied to explore the association between each gene and PFS/OS. Genes significantly correlated with PFS/OS were further correlated with TS-protein expression (Spearman-rank. Unsupervised clustering was applied to all evaluable samples (n = 51 for all 1,030 genes and an overlapping 870-gene subset associated with adenocarcinoma (ADC, n = 47.51/70 tissue-samples (72.9% were evaluable; 9 of 1,030 genes were significantly associated with PFS/OS (unadjusted p < 0.01, genes: Chromosome 16 open reading frame 89, napsin A, surfactant protein B, aquaporin 4, TRAF2- and Nck-interacting kinase, Lysophosphatidylcholine acyltransferase 1, Interleukin 1 receptor type II, NK2 homeobox 1, ABO glycosyl-transferase; expression for all except IL1R2 correlated negatively with nuclear TS-expression (statistically significant for 5/8 genes, unadjusted p<0.01. Cluster-analysis based on 1,030 genes revealed no clear trend regarding PFS/OS; the ADC-based cluster analysis identified 3 groups (n = 21/11/15 with median (95%CI PFS of 8.1(6.9,NE/2.4(1.2,NE/4.4(1.2,NE months and OS of 20.3(17.5,NE/4.3(1.4,NE/8.3(3.9,NE months, respectively.These exploratory gene-expression profiling results describe genes potentially linked to low TS-expression. Nine genes were significantly associated with PFS/OS but could not be

  15. Fellow Profile

    Indian Academy of Sciences (India)

    Fellow Profile. Elected: 1971 Section: Chemistry. Narasimhan, Prof. Palliakaranai Thirumalai Ph.D. (Madras), FNA, FNASc. Date of birth: 28 July 1928. Date of death: 3 May 2013. Specialization: Theoretical Chemistry and Magnetic Resonance Last known address: 1013, Lupine Drive, Sunnyvale, CA 94086, USA. YouTube ...

  16. New-Generation NASA Aura Ozone Monitoring Instrument (OMI) Volcanic SO2 Dataset: Algorithm Description, Initial Results, and Continuation with the Suomi-NPP Ozone Mapping and Profiler Suite (OMPS)

    Science.gov (United States)

    Li, Can; Krotkov, Nickolay A.; Carn, Simon; Zhang, Yan; Spurr, Robert J. D.; Joiner, Joanna

    2017-01-01

    Since the fall of 2004, the Ozone Monitoring Instrument (OMI) has been providing global monitoring of volcanic SO2 emissions, helping to understand their climate impacts and to mitigate aviation hazards. Here we introduce a new-generation OMI volcanic SO2 dataset based on a principal component analysis (PCA) retrieval technique. To reduce retrieval noise and artifacts as seen in the current operational linear fit (LF) algorithm, the new algorithm, OMSO2VOLCANO, uses characteristic features extracted directly from OMI radiances in the spectral fitting, thereby helping to minimize interferences from various geophysical processes (e.g., O3 absorption) and measurement details (e.g., wavelength shift). To solve the problem of low bias for large SO2 total columns in the LF product, the OMSO2VOLCANO algorithm employs a table lookup approach to estimate SO2 Jacobians (i.e., the instrument sensitivity to a perturbation in the SO2 column amount) and iteratively adjusts the spectral fitting window to exclude shorter wavelengths where the SO2 absorption signals are saturated. To first order, the effects of clouds and aerosols are accounted for using a simple Lambertian equivalent reflectivity approach. As with the LF algorithm, OMSO2VOLCANO provides total column retrievals based on a set of predefined SO2 profiles from the lower troposphere to the lower stratosphere, including a new profile peaked at 13 km for plumes in the upper troposphere. Examples given in this study indicate that the new dataset shows significant improvement over the LF product, with at least 50% reduction in retrieval noise over the remote Pacific. For large eruptions such as Kasatochi in 2008 (approximately 1700 kt total SO2/ and Sierra Negra in 2005 (greater than 1100DU maximum SO2), OMSO2VOLCANO generally agrees well with other algorithms that also utilize the full spectral content of satellite measurements, while the LF algorithm tends to underestimate SO2. We also demonstrate that, despite the

  17. New-generation NASA Aura Ozone Monitoring Instrument (OMI) volcanic SO2 dataset: algorithm description, initial results, and continuation with the Suomi-NPP Ozone Mapping and Profiler Suite (OMPS)

    Science.gov (United States)

    Li, Can; Krotkov, Nickolay A.; Carn, Simon; Zhang, Yan; Spurr, Robert J. D.; Joiner, Joanna

    2017-02-01

    Since the fall of 2004, the Ozone Monitoring Instrument (OMI) has been providing global monitoring of volcanic SO2 emissions, helping to understand their climate impacts and to mitigate aviation hazards. Here we introduce a new-generation OMI volcanic SO2 dataset based on a principal component analysis (PCA) retrieval technique. To reduce retrieval noise and artifacts as seen in the current operational linear fit (LF) algorithm, the new algorithm, OMSO2VOLCANO, uses characteristic features extracted directly from OMI radiances in the spectral fitting, thereby helping to minimize interferences from various geophysical processes (e.g., O3 absorption) and measurement details (e.g., wavelength shift). To solve the problem of low bias for large SO2 total columns in the LF product, the OMSO2VOLCANO algorithm employs a table lookup approach to estimate SO2 Jacobians (i.e., the instrument sensitivity to a perturbation in the SO2 column amount) and iteratively adjusts the spectral fitting window to exclude shorter wavelengths where the SO2 absorption signals are saturated. To first order, the effects of clouds and aerosols are accounted for using a simple Lambertian equivalent reflectivity approach. As with the LF algorithm, OMSO2VOLCANO provides total column retrievals based on a set of predefined SO2 profiles from the lower troposphere to the lower stratosphere, including a new profile peaked at 13 km for plumes in the upper troposphere. Examples given in this study indicate that the new dataset shows significant improvement over the LF product, with at least 50 % reduction in retrieval noise over the remote Pacific. For large eruptions such as Kasatochi in 2008 (˜ 1700 kt total SO2) and Sierra Negra in 2005 (> 1100 DU maximum SO2), OMSO2VOLCANO generally agrees well with other algorithms that also utilize the full spectral content of satellite measurements, while the LF algorithm tends to underestimate SO2. We also demonstrate that, despite the coarser spatial and

  18. Effects of gender and physical attractiveness on visual attention to Facebook profiles.

    Science.gov (United States)

    Seidman, Gwendolyn; Miller, Olivia S

    2013-01-01

    The current study examined viewers' gaze while observing Facebook profiles of strangers varying in gender and physical attractiveness. Fifty-one participants viewed four Facebook profiles, a physically attractive and unattractive individual of each gender. Participants' eye movements were tracked as they viewed each profile for 60 seconds. Results showed that participants paid more attention to the physical appearance (main profile photograph) of female than of male profile owners and to the personal information (likes and interests) of male than to female profile owners. Participants spent more time focusing on information that was irrelevant to forming an impression of the profile owner (advertisements) when viewing the profiles of unattractive than attractive individuals, suggesting that they made a greater effort to learn about these individuals.

  19. The influence of humidity fluxes on offshore wind speed profiles

    DEFF Research Database (Denmark)

    Barthelmie, Rebecca Jane; Sempreviva, Anna Maria; Pryor, Sara

    2010-01-01

    extrapolation from lower measurements. With humid conditions and low mechanical turbulence offshore, deviations from the traditional logarithmic wind speed profile become significant and stability corrections are required. This research focuses on quantifying the effect of humidity fluxes on stability corrected...... wind speed profiles. The effect on wind speed profiles is found to be important in stable conditions where including humidity fluxes forces conditions towards neutral. Our results show that excluding humidity fluxes leads to average predicted wind speeds at 150 m from 10 m which are up to 4% higher...... than if humidity fluxes are included, and the results are not very sensitive to the method selected to estimate humidity fluxes....

  20. Country Education Profiles: Algeria.

    Science.gov (United States)

    International Bureau of Education, Geneva (Switzerland).

    One of a series of profiles prepared by the Cooperative Educational Abstracting Service, this brief outline provides basic background information on educational principles, system of administration, structure and organization, curricula, and teacher training in Algeria. Statistics provided by the Unesco Office of Statistics show enrollment at all…

  1. Family Profiles of Cohesion and Parenting Practices and Latino Youth Adjustment.

    Science.gov (United States)

    Bámaca-Colbert, Mayra Y; Gonzales-Backen, Melinda; Henry, Carolyn S; Kim, Peter S Y; Roblyer, Martha Zapata; Plunkett, Scott W; Sands, Tovah

    2017-08-10

    Using a sample of 279 (52% female) Latino youth in 9th grade (M = 14.57, SD = .56), we examined profiles of family cohesion and parenting practices and their relation to youth adjustment. The results of latent profile analyses revealed four family profiles: Engaged, Supportive, Intrusive, and Disengaged. Latino youth in the Supportive family profile showed most positive adjustment (highest self-esteem and lowest depressive symptoms), followed by youth in the Engaged family profile. Youth in the Intrusive and Disengaged profiles showed the lowest levels of positive adjustment. The findings contribute to the current literature on family dynamics, family profiles, and youth psychological adjustment within specific ethnic groups. © 2017 Family Process Institute.

  2. Preliminary results from combined wide-angle and reflection seismic data in the Natal Valley, South Mozambique margin across the Almirante Leite volcanic ridge : MZ2 profile (MOZ3/5 cruise).

    Science.gov (United States)

    Verrier, Fanny; Leprêtre, Angélique; Evain, Mikael; Schnurle, Philippe; Watremez, Louise; Aslanian, Daniel; De Clarens, Philippe; Afonso Dias, Nuno; Afilhado, Alexandra; Leroy, Sylvie; d'Acremont, Elia; Castilla, Raymi; Moulin, Maryline

    2017-04-01

    The study of South Mozambique passive margin is essential to understand its rifting evolution and better constrain kinematic reconstructions model of the Indian Ocean. MOZ3-5 oceanographic cruises (2016) is part of the PAMELA project (PAssive Margin Exploration LAboratory), conducted by TOTAL, IFREMER, in collaboration with Université de Bretagne Occidentale, Université Rennes 1, Université Pierre and Marie Curie, CNRS et IFPEN. These campaigns allowed the acquisition of wide-angle and multichannel seismic data as well as high resolution bathymetric data, dredges, magnetic and gravimetric data. This work focuses on the deep structure of the northern segment of the Natal Valley which was investigated along a 300 km long E-W seismic transect cross-cutting the Almirante Leite volcanic ridge (MZ2 profile). The wide-angle data set is composed of 23 OBS (Ocean Bottom Seismometers) and 19 LSS (Land Seismic Station) spaced by about 12 km and 4-5 km respectively. Forward modelling of the wide-angle data led to a preliminary 2D P-waves velocity model revealing the sedimentary architecture, crustal and lithospherical structures and shallow high velocity material at the volcanic ridge. The aim of this work is to present the first results on the crustal structure from P-waves velocity modeling along the profile MZ2, in order to discuss the sedimentary sequences, the geometry and nature of the crust (oceanic or continental) as well as structures associated with volcanism, and to better understand the margin's evolution. The post-doc of Fanny Verrier is co-funded by TOTAL and IFREMER as part of the PAMELA (Passive Margin Exploration Laboratories) scientific project. Moulin, M., Aslanian, D., 2016. PAMELA-MOZ03 cruise, RV Pourquoi pas ?, http://dx.doi.org/10.17600/16001600 Moulin, M., Evain, M., 2016. PAMELA-MOZ05 cruise, RV Pourquoi pas ?, http://dx.doi.org/10.17600/16009500

  3. An Innovative and Efficient Method for Reverse Design of Wheel-Rail Profiles

    Directory of Open Access Journals (Sweden)

    Rong Chen

    2018-02-01

    Full Text Available Well-designed wheel-rail profiles are not only helpful in achieving expected dynamic vehicle performance but also in extending the service lives of wheels and rails. In this paper, a method for designing wheel-rail profiles is presented based on the rolling radius difference. This method consists of three parts, i.e., the reverse designs of wheel profiles, symmetric rail profiles and non-symmetric rail profiles. A reverse design method for wheel-rail profiles that can obtain smooth profile formed by quadratic curves is established according to the mapping relation between the rolling radius difference and the wheel-rail profile gradient. This reverse design method is verified and an example for optimizing the design of wheel profiles is introduced. Results show that the design method is effective and efficient. The static/dynamic indexes of the optimized wheel profiles matched with CHN60 can be greatly improved. According to the comparative analysis of wheel-rail contact and dynamic performance, when the lateral displacement reaches 6 mm, the maximum contact stress will be distributed evenly and can be decreased by 184.4 MPa compared to that of existing profiles, while the critical speed can be increased by 10.8% and the running stability can be improved by around 7%. It can be seen that this method is useful for the design of new wheel-rail profiles and optimization of existing wheel-rail profiles.

  4. Online Italian fandoms of American TV shows

    Directory of Open Access Journals (Sweden)

    Eleonora Benecchi

    2015-06-01

    Full Text Available The Internet has changed media fandom in two main ways: it helps fans connect with each other despite physical distance, leading to the formation of international fan communities; and it helps fans connect with the creators of the TV show, deepening the relationship between TV producers and international fandoms. To assess whether Italian fan communities active online are indeed part of transnational online communities and whether the Internet has actually altered their relationship with the creators of the original text they are devoted to, qualitative analysis and narrative interviews of 26 Italian fans of American TV shows were conducted to explore the fan-producer relationship. Results indicated that the online Italian fans surveyed preferred to stay local, rather than using geography-leveling online tools. Further, the sampled Italian fans' relationships with the show runners were mediated or even absent.

  5. Quantitative 1D saturation profiles on chalk by NMR

    DEFF Research Database (Denmark)

    Olsen, Dan; Topp, Simon; Stensgaard, Anders

    1996-01-01

    Quantitative one-dimensional saturation profiles showing the distribution of water and oil in chalk core samples are calculated from NMR measurements utilizing a 1D CSI spectroscopy pulse sequence. Saturation profiles may be acquired under conditions of fluid flow through the sample. Results reveal...... that strong saturation gradients exist in chalk core samples after core floods, due to capillary effects. The method is useful in analysis of corefloods, e.g., for determination of capillary pressure functions...

  6. Consultant radiographers: Profile of the first generation

    Energy Technology Data Exchange (ETDEWEB)

    Forsyth, Lesley J., E-mail: l.forsyth@rgu.ac.u [School of Health Sciences, Robert Gordon University, Aberdeen, AB10 7QG (United Kingdom); Maehle, Valerie [Faculty of Health and Social Care, Robert Gordon University, Aberdeen, AB10 7QG (United Kingdom)

    2010-11-15

    Aim: The aim of this research is to examine the profile of first generation consultant radiographers: their demographics, educational backgrounds, qualifications and training, career experience and progression, teaching, lecturing and research activities. Method: Participant recruitment was drawn from the Society and College of Radiographers consultant radiographer group. Data collection involved a self-administered paper based and web based questionnaire. Results: Participant response rate of 55% (n = 11). Conclusions: The profile of the first consultant radiographer cohort reflects a diverse and eclectic mix. While some aspects of their development such as educational background, clinical training and skills enhancement are comparable to nurse consultants, clinical experience and employment history show some differences. Commitment to development of expert clinical skills is evident within the profile of the first generation cohort of consultant radiographers however research and leadership training are not strong features.

  7. Comparative analysis of gene expression profiles of OPN signalling ...

    Indian Academy of Sciences (India)

    The results showed that 23, 33, 59 and 74 genes were significantly changed in the above four kinds of liver diseases, respectively. H-clustering analysis showed that the expression profiles of OPN signalling-related genes were notably different in four kinds of liver diseases. Subsequently, a total of above-mentioned 147 ...

  8. The metabolic profile of long-lived Drosophila melanogaster

    DEFF Research Database (Denmark)

    Sarup, Pernille Merete; Pedersen, Simon Metz Mariendal; Nielsen, Niels Chr

    2012-01-01

    and among age groups. Contrary to results found in a previous study of the transcriptome of these lines the metabolic profile did not show a younger pattern in longevity-selected (LS) flies than in same aged control (C) flies. Rather, many of the metabolites affected by age had levels common to older......We investigated the age-related changes in the metabolic profile of male Drosophila melanogaster and compared the metabolic profile of flies selected for increased longevity to that of control flies of equal age. We found clear differences in metabolite composition between selection regimes...

  9. Effects of Lifestyle Intervention in Pregnancy and Anthropometrics at Birth on Offspring Metabolic Profile at 2.8 Years: Results From the Lifestyle in Pregnancy and Offspring (LiPO) Study

    National Research Council Canada - National Science Library

    Tanvig, Mette; Vinter, Christina A; Jørgensen, Jan S; Wehberg, Sonja; Ovesen, Per G; Beck-Nielsen, Henning; Christesen, Henrik T; Jensen, Dorte M

    2015-01-01

    Context: Maternal obesity and gestational weight gain are linked to offspring adverse metabolic profiles, and lifestyle interventions during pregnancy in obese women may have long-term positive effects on their children...

  10. The effects of polyphenol-rich chokeberry juice on fatty acid profiles and lipid peroxidation of active handball players: results from a randomized, double-blind, placebo-controlled study.

    Science.gov (United States)

    Petrovic, Snjezana; Arsic, Aleksandra; Glibetic, Marija; Cikiriz, Nikola; Jakovljevic, Vladimir; Vucic, Vesna

    2016-10-01

    The effect of polyphenol-rich chokeberry juice consumption on plasma phospholipid fatty acid profiles of 32 active male and female handball players was examined. This randomized, double-blind, placebo-controlled study was conducted during the preparatory training in a closed campus, where 18 players (8 males, 10 females) consumed 100 mL of chokeberry juice, while 14 players (7 males, 7 females) consumed placebo. Lipid status, glucose, thiobarbituric acid reactive substances (TBARS), and percentages of fatty acids were assessed at baseline and at the end of the study. Consumption of chokeberry juice induced decreases of C18:1n-9 and C18:3n-3 in men, but no changes in female players. However, placebo-controlled groups had reduced proportions of mono- (C16:1n-7, C18:1n-7) and polyunsaturated fatty acids (PUFAs: C18:3n-3, C20:5n-3, and C22:4n-6) in males, as well as n-6 PUFAs and total PUFAs in females after consumption. These results indicate that chokeberry juice had a weak impact on attenuating the effect of intensive training in active handball players.

  11. Chronic pain in the Republic of Ireland--community prevalence, psychosocial profile and predictors of pain-related disability: results from the Prevalence, Impact and Cost of Chronic Pain (PRIME) study, part 1.

    Science.gov (United States)

    Raftery, Miriam N; Sarma, Kiran; Murphy, Andrew W; De la Harpe, Davida; Normand, Charles; McGuire, Brian E

    2011-05-01

    The aims of the PRIME study (Prevalence, Impact and Cost of Chronic Pain) were 3-fold: (1) to determine the point prevalence of chronic pain in Ireland; (2) to compare the psychological and physical health profiles of those with and without chronic pain; and (3) to explore a predictive model of pain-related disability. A postal survey of 3136 people was conducted with a representative community-based sample of adults. Measures were obtained for sociodemographic variables, physical and psychological well-being, depressive symptoms, presence of pain, pain severity, pain-related disability, and illness perceptions. Responses were received from 1204 people. The prevalence of chronic pain was 35.5% (95% CI=32.8-38.2) (n=428). No gender difference in prevalence was found. Prevalence of pain increased with age and was associated with manual employment. The most commonly reported site of pain was the lower back (47.6%); however, multiple pain sites was the norm, with more than 80% of participants reporting more than 1 pain site. Approximately 12% of participants were unable to work or were on reduced work hours because of pain. Of those with chronic pain, 15% met the criteria for clinically relevant depression compared with 2.8% of those without pain. A multiple regression analysis, predicting 67% of variance, showed that pain intensity was the strongest predictor of pain-related disability. Depression and illness perceptions were also predictive of pain-related disability, after controlling for the effects of pain intensity. Chronic pain is a prevalent health problem in Ireland and is associated with significant psychological and functional disability. Psychological factors appear to influence the level of pain-related disability. Copyright © 2011 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.

  12. The tolerability profile of clindamycin 1%/benzoyl peroxide 5% gel vs. adapalene 0.1%/benzoyl peroxide 2.5% gel for facial acne: results of a randomized, single-blind, split-face study.

    Science.gov (United States)

    Gonzalez, Pablo; Vila, Ricardo; Cirigliano, Marcela

    2012-12-01

    Topical combination therapy, such as that with fixed-dose clindamycin/benzoyl peroxide (BPO) or adapalene/BPO, is the recommended first-line approach for the treatment of facial acne. To compare the tolerability of clindamycin 1%/BPO 5% gel vs. adapalene 0.1% BPO 2.5% gel for the first 2 weeks of treatment in patients with facial acne. Using a randomized, single-blind, split-face method, 48 patients with acne received both clindamycin/BPO and adapalene/BPO once daily for 2 weeks. The primary endpoint was investigator-assessed tolerability. Treatment efficacy, patient-assessed tolerability and satisfaction, and safety were also investigated. Forty-five patients completed treatment. Investigator-rated scores for erythema, dryness, and peeling were significantly higher with adapalene/BPO than clindamycin/BPO. Patients rated clindamycin/BPO as significantly more tolerable than adapalene/BPO for redness, dryness, burning, itching, and scaling. Investigator Static Global Assessment scores and lesion counts improved with both products, with no significant difference between treatments. Patients' Global Change Assessment showed a statistically significant difference in favor of clindamycin/BPO at week 1, but not week 2. Overall, >80% of patients were "satisfied" or "very satisfied" with treatment at week 2, but 63% of patients stated that they preferred clindamycin/BPO. Both products were well tolerated, with no serious adverse events (AEs), but a post hoc analysis indicated that treatment-related AEs, including irritation, dryness and erythema, were significantly less common with clindamycin/BPO. Clindamycin/BPO had a better tolerability profile than adapalene/BPO during 2 weeks of split-face treatment. Treatment satisfaction was highest with clindamycin/BPO. © 2012 Wiley Periodicals, Inc.

  13. On the Escarpment Wind Profile

    DEFF Research Database (Denmark)

    Jensen, Niels Otto; Peterson, E. W.

    1978-01-01

    It is shown that miscellaneous theories for flow over low ridges give results consistent with each other and that these results can be used to quantify certain observed features of the wind profile downwind from an escarpment......It is shown that miscellaneous theories for flow over low ridges give results consistent with each other and that these results can be used to quantify certain observed features of the wind profile downwind from an escarpment...

  14. Surface glycosylation profiles of urine extracellular vesicles.

    Directory of Open Access Journals (Sweden)

    Jared Q Gerlach

    Full Text Available Urinary extracellular vesicles (uEVs are released by cells throughout the nephron and contain biomolecules from their cells of origin. Although uEV-associated proteins and RNA have been studied in detail, little information exists regarding uEV glycosylation characteristics. Surface glycosylation profiling by flow cytometry and lectin microarray was applied to uEVs enriched from urine of healthy adults by ultracentrifugation and centrifugal filtration. The carbohydrate specificity of lectin microarray profiles was confirmed by competitive sugar inhibition and carbohydrate-specific enzyme hydrolysis. Glycosylation profiles of uEVs and purified Tamm Horsfall protein were compared. In both flow cytometry and lectin microarray assays, uEVs demonstrated surface binding, at low to moderate intensities, of a broad range of lectins whether prepared by ultracentrifugation or centrifugal filtration. In general, ultracentrifugation-prepared uEVs demonstrated higher lectin binding intensities than centrifugal filtration-prepared uEVs consistent with lesser amounts of co-purified non-vesicular proteins. The surface glycosylation profiles of uEVs showed little inter-individual variation and were distinct from those of Tamm Horsfall protein, which bound a limited number of lectins. In a pilot study, lectin microarray was used to compare uEVs from individuals with autosomal dominant polycystic kidney disease to those of age-matched controls. The lectin microarray profiles of polycystic kidney disease and healthy uEVs showed differences in binding intensity of 6/43 lectins. Our results reveal a complex surface glycosylation profile of uEVs that is accessible to lectin-based analysis following multiple uEV enrichment techniques, is distinct from co-purified Tamm Horsfall protein and may demonstrate disease-specific modifications.

  15. Burnout Syndrome Profiles among Teachers (Perfiles del Síndrome de Burnout entre Profesores

    Directory of Open Access Journals (Sweden)

    Sheila Gonçalves Câmara

    2017-12-01

    Full Text Available Burnout Syndrome (BS is a psychological reaction related to chronic occupational stress, both interpersonal and emotional. This study assessed three discriminant profiles of BS in teachers according to psychosocial factors. Profile 1 represents teachers with or without BS; Profile 2 represents teachers with BS or without BS in reference to the dimension guilt; and Profile 3 represents Profile 1 and Profile 2. The sample comprised 679 Brazilian primary school teachers. The instruments used were: sociodemographic data questionnaire; Spanish Burnout Inventory; and Battery of Psychosocial Risk Assessment to assess role ambiguity, social support, self-efficacy, autonomy, role conflict, and overload and intention to leave the profession. Differences between the groups were assessed using discriminant analysis. Results show that the variables that compose the discriminant profiles (Profile 1 and Profile 2 were intention to leave the profession, overload, role conflict, and role ambiguity, with self-efficacy discriminating teachers without BS in Profile 1. Profiles 1 and 2 were discriminated by two determinant factors: role conflict, and intention to leave the profession. The results suggest a need for interventions on psychosocial stressors to prevent burnout.

  16. Histone profiles in cancer.

    Science.gov (United States)

    Riedel, Simone S; Neff, Tobias; Bernt, Kathrin M

    2015-10-01

    While DNA abnormalities have long been recognized as the cause of cancer, the contribution of chromatin is a relatively recent discovery. Excitement in the field of cancer epigenetics is driven by 3 key elements: 1. Chromatin may play an active and often critical role in controlling gene expression, DNA stability and cell identity. 2. Chromatin modifiers are frequent targets of DNA aberrations, in some cancers reaching near 100%. Particularly in cancers with low rates of DNA mutations, the key "driver" of malignancy is often a chromatin modifier. 3. Cancer-associated aberrant chromatin is amenable to pharmacologic modulation. This has sparked the rapidly expanding development of small molecules targeting chromatin modifiers or reader domains, several of which have shown promise in clinical trials. In parallel, technical advances have greatly enhanced our ability to perform comprehensive chromatin/histone profiling. Despite the discovery that distinct histone profiles are associated with prognostic subgroups, and in some instances may point towards an underlying aberration that can be targeted, histone profiling has not entered clinical diagnostics. Even eligibility for clinical trials targeting chromatin hinges on traditional histologic or DNA-based molecular criteria rather than chromatin profiles. This review will give an overview of the philosophical debate around the role of histones in controlling or modulating gene expression and discuss the most common techniques for histone profiling. In addition, we will provide prominent examples of aberrantly expressed or mutated chromatin modifiers that result in either globally or locally aberrant histone profiles, and that may be promising therapeutic targets. Copyright © 2015 Elsevier Inc. All rights reserved.

  17. Beneficial Effects of Coenzyme Q10 Supplementation on Lipid Profile and Intereukin-6 and Intercellular Adhesion Molecule-1 Reduction, Preliminary Results of a Double-blind Trial in Acute Myocardial Infarction

    Science.gov (United States)

    Mohseni, Mona; Vafa, Mohammadreza; Zarrati, Mitra; Shidfar, Farzad; Hajimiresmail, Seyed Javad; Rahimi Forushani, Abbas

    2015-01-01

    Background: The present investigation was aimed to improve the inflammatory factors and lipoproteins concentration in patients with myocardial infarction (MI) by supplementation with coenzyme Q10 (CoQ10). Methods: In a double-blind, placebo-controlled study, we measured serum concentrations of one soluble cell adhesion molecules (intercellular adhesion molecule-1 [ICAM-1]), serum concentration of intereukin-6 (IL-6) and lipid profiles (high-density lipoprotein-cholesterol [HDL-C], low-density lipoprotein-cholesterol [LDL-C], total cholesterol and triglyceride [TG]) in CoQ10 supplementation group (n = 26) compared with placebo group (n = 26) in hyperlipidemic patients with MI. Fifty-two patients were randomized to receive 200 mg/day of CoQ10 or placebo for 12 weeks. Results: There were no significant differences for serum LDL-C, total cholesterol, and TG between two mentioned groups after the intervention. A significant enhancement in serum HDL-C level was observed between groups after the intervention (55.46 ± 6.87 and 44.07 ± 6.99 mg/dl in CoQ10 and placebo groups, respectively P < 0.001). Concentrations of ICAM-1 (415.03 ± 96.89 and 453.38 ± 0.7 ng/dl CoQ10 and placebo groups, respectively, P = 0.001) and IL-6 (11 ± 9.57 and 12.55 ± 8.76 pg/ml CoQ10 and placebo groups, respectively P = 0.001) in serum were significantly decreased in CoQ10 group. Conclusions: Supplementation with CoQ10 in hyperlipidemic patients with MI that have statin therapy has beneficial effects on their aspects of health. PMID:26330989

  18. Safety profiles and efficacy of infliximab therapy in Japanese patients with plaque psoriasis with or without psoriatic arthritis, pustular psoriasis or psoriatic erythroderma: Results from the prospective post-marketing surveillance.

    Science.gov (United States)

    Torii, Hideshi; Terui, Tadashi; Matsukawa, Miyuki; Takesaki, Kazumi; Ohtsuki, Mamitaro; Nakagawa, Hidemi

    2016-07-01

    A large-scale prospective post-marketing surveillance was conducted to evaluate the safety and efficacy of infliximab in Japanese patients with plaque psoriasis, psoriatic arthritis, pustular psoriasis and psoriatic erythroderma. This study was conducted in all psoriasis patients treated with infliximab after its Japanese regulatory approval. Infliximab was administrated at 5 mg/kg at weeks 0, 2 and 6, and every 8 weeks thereafter. Patients were serially enrolled and observed for 6 months to evaluate the safety and efficacy. The safety and efficacy were evaluated in 764 and 746 patients, respectively. Incidences of any and serious adverse drug reactions were 22.51% and 6.94%, respectively, and those of any and serious infusion reactions were 6.15% and 1.31%, respectively, which were comparable with the results in the post-marketing surveillance with 5000 rheumatoid arthritis patients in Japan. Major adverse drug reactions during the follow-up period were infections (5.10%) including pneumonia, cellulitis and herpes zoster, however, no tuberculosis was observed. The safety profiles were equivalent, regardless of the psoriasis types. No new safety problems were identified. The response rates on global improvement and median improvement rate of Psoriasis Area and Severity Index in all patients were 88.0% and 85.0%, respectively. Of note, the efficacy was equivalent for each psoriasis type as well as for each body region. Infliximab was also effective in pustular psoriasis symptoms, joint symptoms and nail psoriasis, as well as improvement of quality of life. Infliximab was confirmed to be highly effective and well tolerated in treating refractory psoriasis, including pustular psoriasis and psoriatic erythroderma. © 2015 Japanese Dermatological Association.

  19. Sparse Representation Denoising for Radar High Resolution Range Profiling

    Directory of Open Access Journals (Sweden)

    Min Li

    2014-01-01

    Full Text Available Radar high resolution range profile has attracted considerable attention in radar automatic target recognition. In practice, radar return is usually contaminated by noise, which results in profile distortion and recognition performance degradation. To deal with this problem, in this paper, a novel denoising method based on sparse representation is proposed to remove the Gaussian white additive noise. The return is sparsely described in the Fourier redundant dictionary and the denoising problem is described as a sparse representation model. Noise level of the return, which is crucial to the denoising performance but often unknown, is estimated by performing subspace method on the sliding subsequence correlation matrix. Sliding window process enables noise level estimation using only one observation sequence, not only guaranteeing estimation efficiency but also avoiding the influence of profile time-shift sensitivity. Experimental results show that the proposed method can effectively improve the signal-to-noise ratio of the return, leading to a high-quality profile.

  20. Preclinical profile of cabazitaxel

    Science.gov (United States)

    Vrignaud, Patricia; Semiond, Dorothée; Benning, Veronique; Beys, Eric; Bouchard, Hervé; Gupta, Sunil

    2014-01-01

    First-generation taxanes have changed the treatment paradigm for a wide variety of cancers, but innate or acquired resistance frequently limits their use. Cabazitaxel is a novel second-generation taxane developed to overcome such resistance. In vitro, cabazitaxel showed similar antiproliferative activity to docetaxel in taxane-sensitive cell lines and markedly greater activity in cell lines resistant to taxanes. In vivo, cabazitaxel demonstrated excellent antitumor activity in a broad spectrum of docetaxel-sensitive tumor xenografts, including a castration-resistant prostate tumor xenograft, HID28, where cabazitaxel exhibited greater efficacy than docetaxel. Importantly, cabazitaxel was also active against tumors with innate or acquired resistance to docetaxel, suggesting therapeutic potential for patients progressing following taxane treatment and those with docetaxel-refractory tumors. In patients with tumors of the central nervous system (CNS), and in patients with pediatric tumors, therapeutic success with first-generation taxanes has been limited. Cabazitaxel demonstrated greater antitumor activity than docetaxel in xenograft models of CNS disease and pediatric tumors, suggesting potential clinical utility in these special patient populations. Based on therapeutic synergism observed in an in vivo tumor model, cabazitaxel is also being investigated clinically in combination with cisplatin. Nonclinical evaluation of the safety of cabazitaxel in a range of animal species showed largely reversible changes in the bone marrow, lymphoid system, gastrointestinal tract, and male reproductive system. Preclinical safety signals of cabazitaxel were consistent with the previously reported safety profiles of paclitaxel and docetaxel. Clinical observations with cabazitaxel were consistent with preclinical results, and cabazitaxel is indicated, in combination with prednisone, for the treatment of patients with hormone-refractory metastatic prostate cancer previously treated

  1. Competency profile of PR professional in sport

    OpenAIRE

    Gazdíková, Lenka

    2013-01-01

    Title: Competency profile of PR professional in sport Objective: The objective is to identify competencies required to a position of PR professional in sport. The aim of a survey is to verify the level of competencies which follows compiling competency profile and comparison with existing profile of PR specialist. Methods: The objective is achieved using survey, interview and analysis of competency models database. Results: The result of this paper is competency profile of PR professional in ...

  2. Trematode hemoglobins show exceptionally high oxygen affinity.

    Science.gov (United States)

    Kiger, L; Rashid, A K; Griffon, N; Haque, M; Moens, L; Gibson, Q H; Poyart, C; Marden, M C

    1998-08-01

    Ligand binding studies were made with hemoglobin (Hb) isolated from trematode species Gastrothylax crumenifer (Gc), Paramphistomum epiclitum (Pe), Explanatum explanatum (Ee), parasitic worms of water buffalo Bubalus bubalis, and Isoparorchis hypselobagri (Ih) parasitic in the catfish Wallago attu. The kinetics of oxygen and carbon monoxide binding show very fast association rates. Whereas oxygen can be displaced on a millisecond time scale from human Hb at 25 degrees C, the dissociation of oxygen from trematode Hb may require a few seconds to over 20 s (for Hb Pe). Carbon monoxide dissociation is faster, however, than for other monomeric hemoglobins or myoglobins. Trematode hemoglobins also show a reduced rate of autoxidation; the oxy form is not readily oxidized by potassium ferricyanide, indicating that only the deoxy form reacts rapidly with this oxidizing agent. Unlike most vertebrate Hbs, the trematodes have a tyrosine residue at position E7 instead of the usual distal histidine. As for Hb Ascaris, which also displays a high oxygen affinity, the trematodes have a tyrosine in position B10; two H-bonds to the oxygen molecule are thought to be responsible for the very high oxygen affinity. The trematode hemoglobins display a combination of high association rates and very low dissociation rates, resulting in some of the highest oxygen affinities ever observed.

  3. Programs to obtain vertical heights from mean sea level and for computing volume of sand/mineral along beaches: A case study with Kalbadevi beach profiling data and results

    Digital Repository Service at National Institute of Oceanography (India)

    Ganesan, P.

    Two programs have been developed to process profile data, for obtaining vertical heights with respect to mean sea level (M.S.L.) and for computation of volume of heavy mineral / sand accumulation or erosion along the beaches. The final output...

  4. Fecal estrone sulfate profile of a sow showing abnormal pregnancy with fetal mummification.

    Science.gov (United States)

    Ohtaki, T; Moriyoshi, M; Nakada, K; Nakao, T

    2000-03-01

    Fecal and plasma E1S of a sow with mummified fetuses, was compared with normal delivery cases. Fecal and plasma fluctuation patterns in E1S were similar. In the sow with fetal mummification both fecal and plasma E1S concentration rapidly decreased after day 80-90 compared to normal farrowing sows. This coincided with the estimated time of fetal death.

  5. Soybean roots grown under heat stress show global changes in their transcriptional and proteomic profiles

    Directory of Open Access Journals (Sweden)

    Oswaldo eValdes-Lopez

    2016-04-01

    Full Text Available Heat stress is likely to be a key factor in the negative impact of climate change on crop production. Heat stress significantly influences the functions of roots, which provide support, water and nutrients to other plant organs. Likewise, roots play an important role in the establishment of symbiotic associations with different microorganisms. Despite the physiological relevance of roots, few studies have examined their response to heat stress. In this study, we performed genome-wide transcriptomic and proteomic analyses on isolated root hairs, which are a single, epidermal cell type, and compared their response to stripped roots. On average, we identified 1,849 and 3,091 genes differentially regulated in root hairs and stripped roots, respectively, in response to heat stress. Our gene regulatory module analysis identified ten key modules that might control the majority of the transcriptional response to heat stress. We also conducted proteomic analysis on membrane fractions isolated from root hairs and compared these responses to stripped roots. These experiments identified a variety of proteins whose expression changed within 3 hours of application of heat stress. Most of these proteins were predicted to play a significant role in thermo-tolerance, as well as in chromatin remodeling and post-transcriptional regulation. The data presented represent an in-depth analysis of the heat stress response of a single cell type in soybean.

  6. Plasma lipid profiling shows similar associations with prediabetes and type 2 diabetes.

    Directory of Open Access Journals (Sweden)

    Peter J Meikle

    Full Text Available The relationship between lipid metabolism with prediabetes (impaired fasting glucose and impaired glucose tolerance and type 2 diabetes mellitus is poorly defined. We hypothesized that a lipidomic analysis of plasma lipids might improve the understanding of this relationship. We performed lipidomic analysis measuring 259 individual lipid species, including sphingolipids, phospholipids, glycerolipids and cholesterol esters, on fasting plasma from 117 type 2 diabetes, 64 prediabetes and 170 normal glucose tolerant participants in the Australian Diabetes, Obesity and Lifestyle Study (AusDiab then validated our findings on 1076 individuals from the San Antonio Family Heart Study (SAFHS. Logistic regression analysis of identified associations with type 2 diabetes (135 lipids and prediabetes (134 lipids, after adjusting for multiple covariates. In addition to the expected associations with diacylglycerol, triacylglycerol and cholesterol esters, type 2 diabetes and prediabetes were positively associated with ceramide, and its precursor dihydroceramide, along with phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. Significant negative associations were observed with the ether-linked phospholipids alkylphosphatidylcholine and alkenylphosphatidylcholine. Most of the significant associations in the AusDiab cohort (90% were subsequently validated in the SAFHS cohort. The aberration of the plasma lipidome associated with type 2 diabetes is clearly present in prediabetes, prior to the onset of type 2 diabetes. Lipid classes and species associated with type 2 diabetes provide support for a number of existing paradigms of dyslipidemia and suggest new avenues of investigation.

  7. Incipient ovarian failure and premature ovarian failure show the same immunological profile

    NARCIS (Netherlands)

    van Kasteren, YM; von Blomberg, M; Hoek, A; de Koning, C; Lambalk, N; van Montfrans, J; Kuik, J

    PROBLEM: Incipient ovarian failure (IOF) is characterized by regular menstrual cycles, infertility and a raised early-follicular FSH in women under 40. IOF might be a precursor or a mitigated form of premature ovarian failure (POF). Disturbances in the immune system may play a role in ovarian

  8. Soybean Roots Grown under Heat Stress Show Global Changes in Their Transcriptional and Proteomic Profiles

    Energy Technology Data Exchange (ETDEWEB)

    Valdés-López, Oswaldo; Batek, Josef; Gomez-Hernandez, Nicolas; Nguyen, Cuong T.; Isidra-Arellano, Mariel C.; Zhang, Ning; Joshi, Trupti; Xu, Dong; Hixson, Kim K.; Weitz, Karl K.; Aldrich, Joshua T.; Paša-Tolić, Ljiljana; Stacey, Gary

    2016-04-25

    Heat stress is likely to be a key factor in the negative impact of climate change on crop production. Roots provide support, water and nutrients to other plant organs. Likewise, roots play an important role in the establishment of symbiotic associations with different microorganisms. Despite the physiological relevance of roots, few studies have examined the response of these plant organs to heat stress. In this study, we performed genome-wide transcriptomic and proteomic analyses on isolated root hairs, which are a single, epidermal cell type, and compared their response to whole roots. We identified 2,013 genes differentially regulated in root hairs in response to heat stress. Our gene regulatory module analysis identified ten, key modules that controlled the majority of the transcriptional response to heat stress. We also conducted proteomic analysis on membrane fractions isolated from roots and root hairs. These experiments identified a variety of proteins whose expression changed within 3 hours of application of heat stress. Most of these proteins were predicted to play a role in thermotolerance, as well as in chromatin remodeling and post-transcriptional regulation. The data presented represent an in-depth analysis of the heat stress response of a single cell type in soybean.

  9. DAST in Flight Showing Diverging Wingtip Oscillations

    Science.gov (United States)

    1980-01-01

    -104 chase plane. When the DAST's mission ended, it deployed a parachute and then a specially equipped Air Force helicopter recovered the drone in mid-air. On the ground, a pilot controlled the DAST vehicle from a remote cockpit while researchers in another room monitored flight data transmitted via telemetry. They made decisions on the conduct of the flight while the DAST was in the air. In case of failure in any of the ground systems, the DAST vehicle could also be flown to a recovery site using a backup control system in the F-104. The DAST Program experienced numerous problems. Only eighteen flights were achieved, eight of them captive (in which the aircraft flew only while still attached to the launch aircraft). Four of the flights were aborted and two resulted in crashes--one on June 12, 1980, and the second on June 1, 1983. Meanwhile, flight experiments with higher profiles, better funded remotely piloted research vehicles took priority over DAST missions. After the 1983 crash, which was caused by a malfunction that disconnected the landing parachute from the drone, the program was disbanded. Because DAST drones were considered expendable, certain losses were anticipated. Managers and researchers involved in other high-risk flight projects gained insights from the DAST program that could be applied to their own flight research programs. The DAST aircraft had a wingspan of 14 feet, four inches and a nose-to-tail length of 28 feet, 4 inches. The fuselage had a radius of about 2.07 feet. The aircraft's maximum loaded weight was about 2,200 pounds. It derived its power from a Continental YJ69-T-406 engine.

  10. Somatic symptom profiles in the general population

    DEFF Research Database (Denmark)

    Eliasen, Marie; Jørgensen, Torben; Schröder, Andreas

    2017-01-01

    . The profiles were further described by their association with age, sex, chronic disease, and self-perceived health. RESULTS: We identified 10 different somatic symptom profiles defined by number, type, and site of the symptoms. The majority of the population (74.0%) had a profile characterized...... by no considerable bothering symptoms, while a minor group of 3.9% had profiles defined by a high risk of multiple somatic symptoms. The remaining profiles were more likely to be characterized by a few specific symptoms. The profiles could further be described by their associations with age, sex, chronic disease...

  11. Social comparison 2.0: examining the effects of online profiles on social-networking sites.

    Science.gov (United States)

    Haferkamp, Nina; Krämer, Nicole C

    2011-05-01

    Through their features--such as profile photographs or the personal vita--online profiles on social-networking sites offer a perfect basis for social comparison processes. By looking at the profile photograph, the user gains an impression of a person's physical attractiveness, and the user's vita shows which career path the person is pursuing. Against the background of Festinger's Social Comparison Theory, the focus of this research is on the effects of online profiles on their recipients. Therefore, qualitative interviews (N = 12) and two online experiments were conducted in which virtual online profiles of either physically attractive or unattractive persons (N = 93) and profiles of users with either high or low occupational attainment (N = 103) were presented to the participants. Although qualitative interviews did not initially give reason to expect online profiles to constitute a basis for comparison processes, results of the experiments proved otherwise. The first study indicates that recipients have a more negative body image after looking at beautiful users than persons who were shown the less attractive profile pictures. Male participants of the second study, who were confronted with profiles of successful males, showed a higher perceived discrepancy between their current career status and an ideal vita than male participants who looked at profiles of less successful persons.

  12. Non-LTE profiles of the Al I autoionization lines. [for solar model atmospheres

    Science.gov (United States)

    Finn, G. D.; Jefferies, J. T.

    1974-01-01

    A non-LTE formulation is given for the transfer of radiation in the autoionizing lines of neutral aluminum at 1932 and 1936 A through both the Bilderberg and Harvard-Smithsonian model atmospheres. Numerical solutions for the common source function of these lines and their theoretical line profiles are calculated and compared with the corresponding LTE profiles. The results show that the non-LTE profiles provide a better match with the observations. They also indicate that the continuous opacity of the standard solar models should be increased in this wavelength region if the center-limb variations of observed and theoretical profiles of these lines are to be in reasonable agreement.

  13. Template-based quaternary structure prediction of proteins using enhanced profile-profile alignments.

    Science.gov (United States)

    Nakamura, Tsukasa; Oda, Toshiyuki; Fukasawa, Yoshinori; Tomii, Kentaro

    2017-11-27

    Proteins often exist as their multimeric forms when they function as so-called biological assemblies consisting of the specific number and arrangement of protein subunits. Consequently, elucidating biological assemblies is necessary to improve understanding of protein function. Template-Based Modeling (TBM), based on known protein structures, has been used widely for protein structure prediction. Actually, TBM has become an increasingly useful approach in recent years because of the increased amounts of information related to protein amino acid sequences and three-dimensional structures. An apparently similar situation exists for biological assembly structure prediction as protein complex structures in the PDB increase, although the inference of biological assemblies is not a trivial task. Many methods using TBM, including ours, have been developed for protein structure prediction. Using enhanced profile-profile alignments, we participated in the 12th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP12), as the FONT team (Group # 480). Herein, we present experimental procedures and results of retrospective analyses using our approach for the Quaternary Structure Prediction category of CASP12. We performed profile-profile alignments of several types, based on FORTE, our profile-profile alignment algorithm, to identify suitable templates. Results show that these alignment results enable us to find templates in almost all possible cases. Moreover, we have come to understand the necessity of developing a model selection method that provides improved accuracy. Results also demonstrate that, to some extent, finding templates of protein complexes is useful even for MEDIUM and HARD assembly prediction. © 2017 The Authors Proteins: Structure, Function and Bioinformatics Published by Wiley Periodicals, Inc.

  14. Profiling and Racial Profiling: An Interactive Exercise

    Science.gov (United States)

    Semple, Philip

    2013-01-01

    Racial Profiling has been recognized as a serious problem that affects many segments of our society and is especially notable in law enforcement. Governments and police services have pronounced that racial profiling is not acceptable and will not be tolerated. They have gone to great lengths in trying to eradicate racial profiling through…

  15. Strong profiling is not mathematically optimal for discovering rare malfeasors

    Energy Technology Data Exchange (ETDEWEB)

    Press, William H [Los Alamos National Laboratory

    2008-01-01

    In a large population of individuals labeled j = 1,2,...,N, governments attempt to find the rare malfeasor j = j, (terrorist, for example) by making use of priors p{sub j} that estimate the probability of individual j being a malfeasor. Societal resources for secondary random screening such as airport search or police investigation are concentrated against individuals with the largest priors. They may call this 'strong profiling' if the concentration is at least proportional to p{sub j} for the largest values. Strong profiling often results in higher probability, but otherwise innocent, individuals being repeatedly subjected to screening. They show here that, entirely apart from considerations of social policy, strong profiling is not mathematically optimal at finding malfeasors. Even if prior probabilities were accurate, their optimal use would be only as roughly the geometric mean between a strong profiling and a completely uniform sampling of the population.

  16. Thirty Stage Annular Centrifugal Contactor Thermal Profile Measurements

    Energy Technology Data Exchange (ETDEWEB)

    David H. Meikrantz; Troy G. Garn; Jack D. Law

    2010-02-01

    A thirty stage 5 cm annular centrifugal contactor cascade has been assembled and tested to obtain thermal profiles during both ambient and heated input conditions of operation. Thermocouples were installed on every stage as well as feed inputs and Real-time data was taken during experiments lasting from two to eight hours at total flow rates of 0.5 to 1.4 liters per minute. Ambient temperature profile results show that only a small amount of heat is generated by the mechanical energy of the contactors. Steady state temperature profiles mimic the ambient temperature of the lab but are higher toward the middle of the cascade. Heated inlet solutions gave temperature profiles with smaller temperature gradients, more driven by the temperature of the inlet solutions than ambient lab temperature. Temperature effects of solution mixing, even at rotor speeds of 4000 rpm, were not measurable.

  17. Examining young children's social competence using functional ability profiles.

    Science.gov (United States)

    McLaughlin, Tara W; Snyder, Patricia A; Algina, James

    2017-08-13

    To explore the use of International Classification of Functioning, Disability, and Health for Children and Youth (ICF-CY) based profiles of children's functional abilities in relation to their social competence. Subgroups based on shared profiles of functional ability were investigated as an alternative or complement to subgroups defined by disability categories. Secondary analysis of a nationally representative data set of young children identified for special education services in the United States was used for the present study. Using five subgroups of children with shared profiles of functional ability, derived from latent class analysis in previous work, regression analyses were used to examine the relationships between social competence and functional abilities profile subgroup membership. Differences among the subgroups were examined using standardized effect sizes. R2 values were used to examine explained variance in social competence in relation to subgroup membership, disability category, and these variables in combination. Functional ability profile subgroup membership was moderately related to children's social competence outcomes: social skills and problem behaviors. Effect sizes showed significant differences between subgroups. Subgroup membership accounted for more variance in social competence outcomes than disability category. The results provide empirical support for the importance of functional ability profiles when examining social competence within a population of young children with disabilities. Implications for Rehabilitation The extent to which children with disabilities experience difficulty with social competence varies by their functional characteristics. Functional ability profiles can provide practitioners and researchers working young children with disabilities important tools to examine social competence and to inform interventions.

  18. Raman lidar water vapor profiling over Warsaw, Poland

    Science.gov (United States)

    Stachlewska, Iwona S.; Costa-Surós, Montserrat; Althausen, Dietrich

    2017-09-01

    Water vapor mixing ratio and relative humidity profiles were derived from the multi-wavelength Raman PollyXT lidar at the EARLINET site in Warsaw, using the Rayleigh molecular extinction calculation based on atmospheric temperature and pressure from three different sources: i) the standard atmosphere US 62, ii) the Global Data Assimilation System (GDAS) model output, and iii) the WMO 12374 radiosoundings launched at Legionowo. With each method, 136 midnight relative humidity profiles were obtained for lidar observations from July 2013 to August 2015. Comparisons of these profiles showed in favor of the latter method (iii), but it also indicated that the other two data sources could replace it, if necessary. Such use was demonstrated for an automated retrieval of water vapor mixing ratio from dusk until dawn on 19/20 March 2015; a case study related to an advection of biomass burning aerosol from forest fires over Ukraine. Additionally, an algorithm that applies thresholds to the radiosounding relative humidity profiles to estimate macro-physical cloud vertical structure was used for the first time on the Raman lidar relative humidity profiles. The results, based on a subset of 66 profiles, indicate that below 6 km cloud bases/tops can be successfully obtained in 53% and 76% cases from lidar and radiosounding profiles, respectively. Finally, a contribution of the lidar derived mean relative humidity to cloudy conditions within the range of 0.8 to 6.2 km, in comparison to clear-sky conditions, was estimated.

  19. Programs to obtain vertical heights from mean sea level and for computing volume of sand/mineral along beaches:A case study with Kalbadevi beach profiling data and results.

    Digital Repository Service at National Institute of Oceanography (India)

    Ganesan, P.

    II) Program for obtaining volume of sand / mineral along profiles from M.S.L. The listing of the software is given in Annexure: I and Annexure :2 of this report. LOGIC AND FLOW DIAGRAM The logic used in first program ?Program to obtain... data points from second set up = (HC1 ? successive staff readings observed from second set up) 5 The logical flow diagram is given below Fig.1 Flow chart for obtaining vertical heights with respect to mean sea...

  20. Estimating Planetary Boundary Layer Heights from NOAA Profiler Network Wind Profiler Data

    Science.gov (United States)

    Molod, Andrea M.; Salmun, H.; Dempsey, M

    2015-01-01

    An algorithm was developed to estimate planetary boundary layer (PBL) heights from hourly archived wind profiler data from the NOAA Profiler Network (NPN) sites located throughout the central United States. Unlike previous studies, the present algorithm has been applied to a long record of publicly available wind profiler signal backscatter data. Under clear conditions, summertime averaged hourly time series of PBL heights compare well with Richardson-number based estimates at the few NPN stations with hourly temperature measurements. Comparisons with clear sky reanalysis based estimates show that the wind profiler PBL heights are lower by approximately 250-500 m. The geographical distribution of daily maximum PBL heights corresponds well with the expected distribution based on patterns of surface temperature and soil moisture. Wind profiler PBL heights were also estimated under mostly cloudy conditions, and are generally higher than both the Richardson number based and reanalysis PBL heights, resulting in a smaller clear-cloudy condition difference. The algorithm presented here was shown to provide a reliable summertime climatology of daytime hourly PBL heights throughout the central United States.

  1. Agricultural Pilot's Audiological Profile

    Directory of Open Access Journals (Sweden)

    Foltz, Lucas

    2010-09-01

    Full Text Available Introduction: The agricultural airplane pilot are daily exposed to intense noises, being susceptible to the noise-induced hearing loss (NIHL and its auditory and extra auditory effects. Objective: To analyze the audiological profile of this population, verifying the work's influence on its hearing. Method: It was realized a retrospective, individual, observational, and cross-sectional study through the data obtained by means of a questionnaire and audiometric thresholds of 41 agricultural pilots. To the statistical analysis were utilized the chi-square, Spearman, and Wilcoxon tests with significance level of 5%. Results: It was verified that 95,1% of the pilots use PPE ( personal protective equipment during flight and 58,5% have contact with pesticides. More than half of individuals referred to feel auditory and extra auditory symptoms, being the buzz the more frequent (29,1%. It has the occurrence of 29,3% of NIHL suggestive hearing loss and 68,3% of normality, taking this presence of unilateral notch in 24,4% and bilateral notch in 31,7%. It was found correlation statistically significant in the associations between time of service and the average of the acute frequencies in the right ear (p=0038, and in the left ear (p=0,010. It has a statistical tendency in the association between audiometric configuration and contact with pesticides (p=0,088. Conclusion: The hearing loss prevalence in this study was showed high. More than half of the sample has normal audiometric thresholds with notch configuration. Such data lead to the conclusion that the agricultural pilots, even with PPE use, they still suffer with the damages caused by noise, needing best proposals of hearing loss prevention.

  2. Le profil SVP : sismique au voisinage du puits The Wvp (Well Vicinity Profiling Stack

    Directory of Open Access Journals (Sweden)

    Blondin E.

    2006-11-01

    Full Text Available L'augmentation de l'investigation latérale du PSV (profil sismique vertical et le calage de la sismique de surface au puits peuvent être améliorés en combinant un profil sismique réflexion de surface d'une mise en oeuvre particulière (à base réceptrice fixe appelé SVP (sismique au voisinage du puits et un profil sismique vertical. Dans cette mise en oeuvre combinée, la sonde n'est immobilisée que pour le temps d'acquisition du PSV. L'utilisation d'une base sismique réceptrice fixe rend la mise en oeuvre du profil SVP légère et peu onéreuse. Les résultats obtenus montrent que les résolutions verticales du profil SVP et du PSV sont équivalentes. L'investigation latérale de cette mise en oeuvre mixte est de plusieurs centaines de mètres quelle que soit la profondeur. Cette recherche méthodologique a été réalisée en collaboration avec Gaz de France. Increasing the lateral range of investigation of VSP (vertical seismic profiling and the calibration of surface seismic profiles with well geological horizons can be improved by combining a surface seismic profile having a specific implementation (with a fixed receiving base called WVP (well vicinity profiling and a vertical seismic profile. In this combined implementation, the drilling rig is immobilized only for the acquisition time of the VSP. The use of a fixed seismic receiving base makes the implementation of the WVP stack light and inexpensive. The results obtained show that the vertical resolutions of the WVP stack and of the VSP are the same. The lateral range of investigation of this mixed implementation is several hundred meters no matter what the depth may be. This methodological research was done in collaboration with Gaz de France.

  3. Laquinimod Safety Profile

    DEFF Research Database (Denmark)

    Sørensen, Per Soelberg; Comi, Giancarlo; Vollmer, Timothy L

    2017-01-01

    the safety profile of laquinimod versus placebo. Adverse events (AEs), laboratory value changes, and potential risks identified in preclinical studies were evaluated in participants in ALLEGRO and BRAVO treated with at least one dose of laquinimod or matching placebo (1:1 random assignment). RESULTS......: In total, 1988 patients received at least one dose of study drug (laquinimod: n = 983 [mean ± SD duration, 639 ± 190 days]; placebo: n = 1005 [mean ± SD duration, 627 ± 198 days]). Early terminations due to AEs were infrequent (laquinimod: 6.4%; placebo: 4.7%). Death was reported in four patients...... laquinimod studies demonstrate a safety profile comprising benign or manageable AEs and asymptomatic laboratory findings with a clear temporal pattern. Potential risks noted in preclinical studies were not observed....

  4. Molecular dynamics simulations and free energy profile of ...

    Indian Academy of Sciences (India)

    Also, the convergence in time of the free energy profile of the Paracetamol along a DPPC bilayer normal was calculated by umbrella sampling method. From the obtained results, it can be concluded that neutral form of Paracetamol shows a generally similar behaviour in DPPC and DMPC lipid bilayers. It was shown that the ...

  5. Petroleum Hydrocarbons Contamination Profile of Ochani Stream in ...

    African Journals Online (AJOL)

    Petroleum hydrocarbon contamination profile, heavy metals and some physicochemical parameters were investigated in Ochani Stream site in Ejamah Ebubu, Eleme Local Government Area of Rivers State. The results show that a major crude oil spillage occurred at Ejamah Ebubu, Rivers State, Nigeria approximately 30 ...

  6. Proximate composition and amino acid profile of rice husk ...

    African Journals Online (AJOL)

    Native rice husk (NRH) was fermented with Pleurotus ostreatus for 7, 14 and 21 days to improve the nutritional values. The proximate composition and amino acid profiles were determined. The results showed that crude fibre (CF), nitrogen free extract (NFE), acid detergent fibre (ADF), and neutral detergent fibre (NDF) were ...

  7. Antimicrobial susceptibility profile of Listeria species isolated from ...

    African Journals Online (AJOL)

    The antimicrobial susceptibility profile of L. monocytogenes and other Listeria species isolated from some ready-to-eat (RTE) foods sold in Kano metropolis, north-western Nigeria was carried out using disc-diffusion method. The results obtained showed that L. monocytogenes was moderately susceptible to all the ...

  8. Frequency of MMPI Profile Types in Three Chronic Illness Populations.

    Science.gov (United States)

    Naliboff, Bruce D.; And Others

    1983-01-01

    Developed sorting rules to identify Minnesota Multiphasic Personality Inventory configurations and applied these to patients with chronic low back pain, migraine headaches, hypertension, and diabetes (N=157). Results showed that profile types did not differ in frequency among the groups but were more elevated in the back pain patients. (LLL)

  9. Profile of congenital malformations in Sagamu, Nigeria | Oloyede ...

    African Journals Online (AJOL)

    The profile of congenital malformations seen in Sagamu, Southwestern Nigeria during a period of eight years is described. The result of this retrospective cross sectional study showed an incidence of congenital malformation of 10.7 per 1000 live births. Low order of parity (1 and 2) was mostly affected (65.8%), and 80.0% of ...

  10. Leukocyte Profile in HIV Positive Adults in Owerri, Nigeria ...

    African Journals Online (AJOL)

    Leukocyte profile of 102 HIV positive asymptomatic adults (aged 18 – 56 years) in Owerri was compared with that of 80 HIV-negative adults (aged 22 – 50 years). The goal was to define early indicators of HIV-pathophysiology using leukocyte distribution pattern. Results show a definite pattern of leukopenia, relative ...

  11. Nutritional profiles of tiger Nut ( Cyperus esculentus ) plant organs ...

    African Journals Online (AJOL)

    This study was carried out to determine major nutrient profiles changes of tiger nut plant during its growth period. The plant leaves, roots, tuber moisture, starch, fat and protein were analyzed by oven drying, enzymatic hydrolysis, glucose assay, soxhlet extraction and kjeldahl methods. The results show the moisture content ...

  12. Total lipid profile with aqueous fruit extract of Solanum macrocarpum ...

    African Journals Online (AJOL)

    The results show that the aqueous fruit extract of the plant may be capable of reducing circulating lipids in high cholesterol-diet fed rats. However, more work still needs to be done on this as the changes in the serum lipid profile were not significant so as to determine whether the plant either inhibits lipid biosynthesis or ...

  13. Assimilation of NUCAPS Retrieved Profiles in GSI for Unique Forecasting Applications

    Science.gov (United States)

    Berndt, Emily Beth; Zavodsky, Bradley; Srikishen, Jayanthi; Blankenship, Clay

    2015-01-01

    Hyperspectral IR profiles can be assimilated in GSI as a separate observation other than radiosondes with only changes to tables in the fix directory. Assimilation of profiles does produce changes to analysis fields and evidenced by: Innovations larger than +/-2.0 K are present and represent where individual profiles impact the final temperature analysis.The updated temperature analysis is colder behind the cold front and warmer in the warm sector. The updated moisture analysis is modified more in the low levels and tends to be drier than the original model background Analysis of model output shows: Differences relative to 13-km RAP analyses are smaller when profiles are assimilated with NUCAPS errors. CAPE is under-forecasted when assimilating NUCAPS profiles, which could be problematic for severe weather forecasting Refining the assimilation technique to incorporate an error covariance matrix and creating a separate GSI module to assimilate satellite profiles may improve results.

  14. Program analysis and presentation of results of the profiles and depth dose rates obtained with the PTW software MC{sub 2} MEPHYSTO; Programa de analisis y presentacion de resultados de los perfiles y porcentajes de dosis en profundidad adquiridos con el software MEPHYSTO MC2 de PTW

    Energy Technology Data Exchange (ETDEWEB)

    Tato de la Cuevas, F.

    2011-07-01

    In the periodic quality control of linear accelerators is usually included acquisition and analysis of profiles and PDDs (percentage depth dose). In the protocol of Quality Control of electron accelerators for clinical use of the proposed analysis SEFM 4 Profiles for each of the energies used clinically. This involves a large number of curves to be analyzed and the subsequent introduction of the parameters in a spreadsheet or similar for your assessment as to the reference state. We have developed a program that analyzes the curves acquired by mcc Mephysto PTW software and presents the results of that analysis in a spreadsheet.

  15. The Physics of Equestrian Show Jumping

    Science.gov (United States)

    Stinner, Art

    2014-01-01

    This article discusses the kinematics and dynamics of equestrian show jumping. For some time I have attended a series of show jumping events at Spruce Meadows, an international equestrian center near Calgary, Alberta, often referred to as the "Wimbledon of equestrian jumping." I have always had a desire to write an article such as this…

  16. On Active Current Selection for Lagrangian Profilers

    Directory of Open Access Journals (Sweden)

    J. Jouffroy

    2013-01-01

    Full Text Available Autonomous Lagrangian profilers are now widely used as measurement and monitoring platforms, notably in observation programs as Argo. In a typical mode of operation, the profilers drift passively at their parking depthbefore making a vertical profile to go back to the surface. This paperpresents simple and computationally-efficient control strategies to activelyselect and use ocean currents so that a profiler can autonomously reach adesired destination. After briefly presenting a typical profiler andpossible mechanical modifications for a coastal environment, we introducesimple mathematical models for the profiler and the currents it will use. Wethen present simple feedback controllers that, using the direction of thecurrents and taking into account the configuration of the environment(coastal or deep-sea, is able to steer the profiler to any desiredhorizontal location. To illustrate the approach, a few results are presentedusing both simulated currents and real current velocity profiles from theNorth Sea.

  17. Measurement of inner and/or outer profiles of pipes using ring beam devices

    Science.gov (United States)

    Wakayama, T.; Yoshizawa, T.

    2009-11-01

    Inner profile measurement is an important matter in such fields as medicine, dentistry and anthropology as well as mechanical engineering and industry. Here we propose a measurement method for inner diameter of pipes and/or holes. The key device in this technique is a ring beam device which consists of a conical mirror and a laser diode. And the fundamental principle is based on optical sectioning without any contact probe. The optically sectioned profile of an inner wall of a pipe-like object is analyzed to give the inner profile in addition to the inner diameter. This optical instrument with a simple and small configuration is now under development for practical uses. In the hitherto-tried experimental works, the availability of this instrument has been highly evaluated and usability for practical applications is expected, especially, for measurement and inspection of mechanical components and elements besides pipes. This ring beam device consisting of a conical mirror and a LD is assembled to form a disklike light beam sheet. We show measurement result of pipes and holes, and, at the same time, report a compact inner profile measuring instrument. Both the ring beam device and a miniaturized CCD camera are fabricated in a glass tube. Availability of this instrument is shown by measuring the inner profiles of various pipes. In response to this trial, there appeared a strong request that not only the internal but external profiles should be measured simultaneously. Therefore we propose an improved method for measuring the external profile in addition to the internal profile. In our arrangement, one pair of concaved conical mirrors is used for the external profile measurement. In combination with the inner profile measurement technique, simultaneous measurement of the inner and outer profiles becomes attainable. A measurement result on a bevel gear shows availability of newly proposed principle. Now we are aiming to realize simultaneous measurement of the internal

  18. Voyeurismo Televisivo, Reality Shows e Brasilidade Televisiva

    Directory of Open Access Journals (Sweden)

    Suzana Kilpp

    2008-12-01

    Full Text Available In the last years we watched a boom of reality shows in the media and also in the academic production specialized in this subject. It remains, however, a epistemological gap related to the aesthetic and techniques (which are related to the televisions grammars that TV uses in these programs to enunciate ethics directions to its own voyeurism, that goes far beyond reality shows, having repercussions on social imaginary of transparency and surveillance, and the redesign of public and private spaces. In this gap, the article points out the debate of Brazilian reality shows in the perspective of the televisions grammars.

  19. Preclinical profile of cabazitaxel

    Directory of Open Access Journals (Sweden)

    Vrignaud P

    2014-10-01

    safety profiles of paclitaxel and docetaxel. Clinical observations with cabazitaxel were consistent with preclinical results, and cabazitaxel is indicated, in combination with prednisone, for the treatment of patients with hormone-refractory metastatic prostate cancer previously treated with docetaxel. In conclusion, the demonstrated activity of cabazitaxel in tumors with innate or acquired resistance to docetaxel, CNS tumors, and pediatric tumors made this agent a candidate for further clinical evaluation in a broader range of patient populations compared with first-generation taxanes. Keywords: XRP6258, CNS tumors, mCRPC, pediatric tumor, taxane resistance, xenograft

  20. Diabetes Drug Shows Promise Against Parkinson's

    Science.gov (United States)

    ... fullstory_167612.html Diabetes Drug Shows Promise Against Parkinson's Byetta improved symptoms of motor disease in small, ... may do double duty as a treatment for Parkinson's disease, a new study suggests. "This is a ...

  1. UV Photography Shows Hidden Sun Damage

    Science.gov (United States)

    ... mcat1=de12", ]; for (var c = 0; c UV photography shows hidden sun damage A UV photograph gives ... developing skin cancer and prematurely aged skin. Normal photography UV photography 18 months of age: This boy's ...

  2. Career development at London Vet Show.

    Science.gov (United States)

    2016-09-03

    Are you considering a career change? Perhaps you want help to develop within your current role? Either way, you will find a relevant session in the BVA Career Development stream at the London Vet Show in November. British Veterinary Association.

  3. Laser Therapy Shows Promise Against Eye 'Floaters'

    Science.gov (United States)

    ... page: https://medlineplus.gov/news/fullstory_167324.html Laser Therapy Shows Promise Against Eye 'Floaters' These spots ... 2017 THURSDAY, July 20, 2017 (HealthDay News) -- A laser treatment can reduce spots in people's vision known ...

  4. A STUDY OF LIPID PROFILE IN PREDIABETES

    Directory of Open Access Journals (Sweden)

    Manoj

    2016-06-01

    Full Text Available BACKGROUND Lipid abnormalities are common in diabetes mellitus and play an important role in acceleration of atherosclerosis leading to increased cardiovascular diseases. Due to increasing burden of diabetes, it is becoming important to identify dyslipidaemia in high-risk state for diabetes especially prediabetes so that early intervention can reduce cardiovascular risk. AIM To study lipid profile in prediabetes individuals. METHODS This study was a cross-sectional case control study which included 107 prediabetes and 101 healthy controls. Lipid profile of prediabetes and controls were measured and statistically analysed. RESULT Total cholesterol, LDL, triglycerides, VLDL, TG/HDL ratio, and LDL/HDL ratio were significantly high whereas HDL was significantly low in prediabetes subjects as compared to controls. CONCLUSION This study showed significant lipid abnormalities in prediabetes subjects. Because of these they are at high risk of developing atherosclerotic cardiovascular diseases. Therefore, proper screening and appropriate therapy of these conditions becomes important.

  5. Geometrical theory to predict eccentric photorefraction intensity profiles in the human eye

    Science.gov (United States)

    Roorda, Austin; Campbell, Melanie C. W.; Bobier, W. R.

    1995-08-01

    In eccentric photorefraction, light returning from the retina of the eye is photographed by a camera focused on the eye's pupil. We use a geometrical model of eccentric photorefraction to generate intensity profiles across the pupil image. The intensity profiles for three different monochromatic aberration functions induced in a single eye are predicted and show good agreement with the measured eccentric photorefraction intensity profiles. A directional reflection from the retina is incorporated into the calculation. Intensity profiles for symmetric and asymmetric aberrations are generated and measured. The latter profile shows a dependency on the source position and the meridian. The magnitude of the effect of thresholding on measured pattern extents is predicted. Monochromatic aberrations in human eyes will cause deviations in the eccentric photorefraction measurements from traditional crescents caused by defocus and may cause misdiagnoses of ametropia or anisometropia. Our results suggest that measuring refraction along the vertical meridian is preferred for screening studies with the eccentric photorefractor.

  6. Positron depth profiling of the structural and electronic structure transformations of hydrogenated Mg-based thin films

    NARCIS (Netherlands)

    Eijt, S.W.H.; Kind, R.; Singh, S.; Schut, H.; Legerstee, W.J.; Hendrikx, R.W.A.; Svetchnikov, V.L.; Westerwaal, R.J.; Dam, B.

    2009-01-01

    We report positron depth-profiling studies on the hydrogen sorption behavior and phase evolution of Mg-based thin films. We show that the main changes in the depth profiles resulting from the hydrogenation to the respective metal hydrides are related to a clear broadening in the observed electron

  7. Validation of the Concept of a Common Typical Time of Disease Duration for Hepatocellular Carcinoma Patients Using the Fisher Information Processing of Tumor Imaging Results Combined With Network Phenotyping Strategy Quantification of Individual Patient Clinical Profile Patterns.

    Science.gov (United States)

    Pančoška, Petr; Skála, Lubomír; Nešetřil, Jaroslav; Carr, Brian I

    2015-08-01

    A primary goal of current clinical cancer research is the identification of prognostic tumor subtypes. It is increasingly clear that tumor growth depends on both internal tumor factors, and factors that are external to the tumor, such as microenvironment. We recently showed that parameter values alone are less important than the patterns of all patient parameters together for the identification of prognostic subtypes and have identified a network phenotyping strategy method to quantitatively describe the dependency of the tumor on the environment, to characterize hepatocellular carcinoma (HCC) subtypes. We have also shown that information about tumor mass together with patterns of other prognostic factors is related to survival. We now use a different patient cohort to validate this prognostic approach. A main finding is our identification of a common time of total disease duration (TDD) for every HCC patient. Clinical prognosis at the time of baseline patient evaluation is then calculable as the difference between TDD and the time from disease onset to diagnosis (T(onset)). We show that the total pattern of all parameter values and the differences in the relationships between this pattern and a reference pattern that, together with the tumor mass, best reflects the patient's prognosis at baseline. Our approach led us to identify 15 different composite HCC subtypes. Our results highlight the nearly identical TDD in all patients, which must therefore be a characteristic of the HCC disease, as opposed to the variable quantity of T(onset), which is impacted by multiple macro- and micro-environmental factors. Copyright © 2015 Elsevier Inc. All rights reserved.

  8. 2008 LHC Open Days Physics: the show

    CERN Document Server

    2008-01-01

    A host of events and activities await visitors to the LHC Open Days on 5 and 6 April. A highlight will be the physics shows funded by the European Physical Society (EPS), which are set to surprise and challenge children and adults alike! School children use their experience of riding a bicycle to understand how planets move around the sun (Copyright : Circus Naturally) Participating in the Circus Naturally show could leave a strange taste in your mouth! (Copyright : Circus Naturally) The Rino Foundation’s experiments with liquid nitrogen can be pretty exciting! (Copyright: The Rino Foundation)What does a bicycle have in common with the solar system? Have you ever tried to weigh air or visualise sound? Ever heard of a vacuum bazooka? If you want to discover the answers to these questions and more then come to the Physics Shows taking place at the CERN O...

  9. Molecular typing of Salmonella from Sergipe, Northeastern Brazil, showing the epidemiological relationship between poultry and human infection.

    Science.gov (United States)

    Góis, P B P; Carneiro, M R P; Jain, S; Santos, M I S; Batista, M V A; Cândido, A L

    2015-09-25

    Randomly amplified polymorphic DNA (RAPD) has been widely used for epidemiological and phylogenetic purposes ow-ing to its rapidity and efficiency. The aim of this study was to perform genome typing of Salmonella samples isolated from different sources by RAPD profiling. Thirty-three Salmonella samples from the bacterial collection of the Laboratório de Virologia Comparada, Departamento de Morfologia, Universidade Federal de Sergipe, Brazil, and two standard samples were used. RAPD profiling was conducted using six primers of the Ready-To-Go RAPD system. The amplified products were electro-phoresed on 5% polyacrylamide gel and silver-stained. RAPD analysis resulted in reproducible and stable banding patterns and showed high genetic diversity among the isolated strains. The Primer P1-generated dendrogram showed an epidemiologic relationship between the human and poultry isolated samples, highlighting the usefulness of RAPD for molecular typing and epidemiological studies.

  10. Satire og diskurs i The Daily Show

    OpenAIRE

    Rygaard, Alexander Leicht; Dall, Christoffer; Jakobsen, Sebastian Rasch; Duvander, Søren; Knøster, Morten; Kirkegaard, Elissa Valles; Wallin, Dea

    2014-01-01

    This project examines how The Daily Show and its host Jon Stewart takes part in the American political debate. Furthermore, the term satire is explained by linguist Paul Simpson who reckons that satire is not as much a genre as it is a practice of discourse. By the use of detailed statistics by Gallup and Pew Research Center, a picture of the American media and its consumers is provided to explain why The Daily Show has gained more viewership through the years. Throughout a critical discourse...

  11. Variation of Chemical Elements and their Associations in Laterite Soil Profile

    Directory of Open Access Journals (Sweden)

    M Tufaila

    2015-05-01

    Full Text Available The objective of this study was to evaluate the variations of twelve chemical elements (Ni, Fe, Co, Mg, Mn, Al, Si, Zn, Ti, P, Ca and Cr in laterite soil profiles from Wantulasi area in South East Sulawesi Province of Indonesia. Eighty four (84 samples of three profiles (i.e. each profile consists of eight samples had been used to study their variations in soil using the X-Ray Fluorescence (XRF. Results analysis of the chemical elements content in three profile using XRF indicated that there were good correlationsbetween the chemical elements in the soil profile with the significant correlations were found in Ni and Fe, Ni and Si, Ti and P, Fe and Al, and Co and Mn, respectively. On the other hand,the results of study showed that the variations of the chemical elements could be related to the enrichment and translocation of the elements in soil profile and also their possibilities to be related with a given chemical elements in soil profile. Therefore, we suggest that the observed patterns in chemical elements with a good correlation in laterite profile can be used as proxies to integrate the evaluation of the chemical and physical weathering process based on the elements characteristics in soil profiles.

  12. The Sensitivity Analysis of Axial Pressure Tube Creep Profile for Dryout Power in PHWR

    Energy Technology Data Exchange (ETDEWEB)

    Ryu, Euiseung; Kim, Youngae [KHNP CRI, Daejeon (Korea, Republic of)

    2016-10-15

    The Stern Laboratory performed the CHF tests with only one axial pressure tube creep profile per 3.3%, 5.1% peak crept channel and made CHF correlation including creep factor from the CHF test results. Wolsong nuclear power plants also have utilized the same CHF correlation derived by CNL. Pressure tube diameter creep rate is function of fast neutron, coolant temperature, and coolant pressure in a channel. It means that various axial pressure tube creep profiles exist in PHWR due to the history of operating conditions. Usually, CHF correlation is used during ROP(Regional Overpower Protection) Trip Setpoint Analysis or Safety Analysis in PHWR. The sensitivity analysis for CHF effects using various creep profiles is needed. This paper summarizes the comparison results of dryout power between CHF test creep profile and estimated creep profiles of Wolsong units. The effect of axial pressure tube creep profile for dryout power in fuel channel is evaluated by using Stern Lab. CHF test creep profile and 380 channel creep profiles of Wolsong. The dryout powers at 3.3% and 5.1% test conditions are slightly smaller when using 380 Wolsong channels creep profiles. These also show that the simulated dryout powers maintain consistency regardless of flow conditions.

  13. Long-term Results from the Empowering a Multimodal Pathway Toward Healthy Youth Program, a Multimodal School-Based Approach, Show Marked Reductions in Suicidality, Depression, and Anxiety in 6,227 Students in Grades 6-12 (Aged 11-18).

    Science.gov (United States)

    Silverstone, Peter H; Bercov, Marni; Suen, Victoria Y M; Allen, Andrea; Cribben, Ivor; Goodrick, Jodi; Henry, Stu; Pryce, Catherine; Langstraat, Pieter; Rittenbach, Katherine; Chakraborty, Samprita; Engles, Rutger C; McCabe, Christopher

    2017-01-01

    Here, we report on findings from a 15-month follow-up of a school-based program called Empowering a Multimodal Pathway Toward Healthy Youth (EMPATHY). This was primarily intended to reduce suicidal thinking in pre-teens, adolescents, and youth students aged 11-18 in middle schools (Grades 6-8) and high SCHOOLS (Grades 9-12). It also aimed to reduce depression and anxiety. The EMPATHY multimodal program consisted of repeated data collection, identification of a high-risk group, a rapid intervention for this high-risk group including offering supervised online cognitive behavioral therapy (CBT) program, a universal CBT intervention for those in Grades 6-8, a variety of interactions with trained staff ("Resiliency Coaches"), and referral to external medical and psychiatric services where appropriate. There were four time-points at which assessments were made: baseline, 3, 7, and 15 months. Here, we report cross-sectional findings over 15 months in a total of 6,227 students who were assessed at least once during the study period. Additionally, we report longitudinal findings from the 1,884 students who completed all 4 assessments. Our results found highly statistically significant decreases in suicidality rates, with the percentage of the total school population who were actively suicidal decreasing from 4.4% at baseline (n = 143 of 3,244) to 2.8% at 15 months (n = 125 of 4,496) (p < 0.001). There were also highly statistically significant reductions in depression and anxiety scores at each time-point. Thus, Mean Depression scores at baseline for the entire student population were 3.73 ± 3.87 (n = 3,244) at baseline and decreased to 3.22 ± 3.52 (n = 4,496) (p < 0.001). Since most students were not depressed, whole population changes such as this may indicate impact in many areas. In the longitudinal analysis of students who completed all four assessments, there were also highly statistically significant improvements in depression

  14. Long-term Results from the Empowering a Multimodal Pathway Toward Healthy Youth Program, a Multimodal School-Based Approach, Show Marked Reductions in Suicidality, Depression, and Anxiety in 6,227 Students in Grades 6–12 (Aged 11–18

    Directory of Open Access Journals (Sweden)

    Peter H. Silverstone

    2017-05-01

    Full Text Available Here, we report on findings from a 15-month follow-up of a school-based program called Empowering a Multimodal Pathway Toward Healthy Youth (EMPATHY. This was primarily intended to reduce suicidal thinking in pre-teens, adolescents, and youth students aged 11–18 in middle schools (Grades 6–8 and high SCHOOLS (Grades 9–12. It also aimed to reduce depression and anxiety. The EMPATHY multimodal program consisted of repeated data collection, identification of a high-risk group, a rapid intervention for this high-risk group including offering supervised online cognitive behavioral therapy (CBT program, a universal CBT intervention for those in Grades 6–8, a variety of interactions with trained staff (“Resiliency Coaches”, and referral to external medical and psychiatric services where appropriate. There were four time-points at which assessments were made: baseline, 3, 7, and 15 months. Here, we report cross-sectional findings over 15 months in a total of 6,227 students who were assessed at least once during the study period. Additionally, we report longitudinal findings from the 1,884 students who completed all 4 assessments. Our results found highly statistically significant decreases in suicidality rates, with the percentage of the total school population who were actively suicidal decreasing from 4.4% at baseline (n = 143 of 3,244 to 2.8% at 15 months (n = 125 of 4,496 (p < 0.001. There were also highly statistically significant reductions in depression and anxiety scores at each time-point. Thus, Mean Depression scores at baseline for the entire student population were 3.73 ± 3.87 (n = 3,244 at baseline and decreased to 3.22 ± 3.52 (n = 4,496 (p < 0.001. Since most students were not depressed, whole population changes such as this may indicate impact in many areas. In the longitudinal analysis of students who completed all four assessments, there were also highly statistically significant

  15. TV-Show Retrieval and Classification

    NARCIS (Netherlands)

    Musto, C.; Narducci, F.; Lops, P.; Semeraro G.; Gemmis, M. de; Barbieri, M.; Korst, J.H.M.; Pronk, S.P.P.; Clout, R.A.W.

    2012-01-01

    Recommender systems are becoming popular tools to aid users in finding interesting and relevant TV-shows and other digital video assets,based on implicitly learned user preferences. In this context, a common assumption is that user preferences can be specified by program types (movie, sports, ...)

  16. The Last Great American Picture Show

    NARCIS (Netherlands)

    Elsaesser, Thomas; King, Noel; Horwath, Alexander

    2004-01-01

    The Last Great American Picture Show brings together essays by scholars and writers who chart the changing evaluations of the American cinema of the 1970s, sometimes referred to as the decade of the lost generation, but now more and more recognized as the first New Hollywood, without which the

  17. Mike Pentz showing visitors around CESAR

    CERN Multimedia

    CERN PhotoLab

    1964-01-01

    Mike Pentz, leader of the CESAR Group, shows visitors around the 2 MeV electron storage ring. Here they are in the vault of the injector (a 2 MV van de Graaff generator), next to the 2 beam lines, one leading to the ring, the other to the spectrometer.

  18. Show Them You Really Want the Job

    Science.gov (United States)

    Perlmutter, David D.

    2012-01-01

    Showing that one really "wants" the job entails more than just really wanting the job. An interview is part Broadway casting call, part intellectual dating game, part personality test, and part, well, job interview. When there are 300 applicants for a position, many of them will "fit" the required (and even the preferred) skills listed in the job…

  19. Laser entertainment and light shows in education

    Science.gov (United States)

    Sabaratnam, Andrew T.; Symons, Charles

    2002-05-01

    Laser shows and beam effects have been a source of entertainment since its first public performance May 9, 1969, at Mills College in Oakland, California. Since 1997, the Photonics Center, NgeeAnn Polytechnic, Singapore, has been using laser shows as a teaching tool. Students are able to exhibit their creative skills and learn at the same time how lasers are used in the entertainment industry. Students will acquire a number of skills including handling three- phase power supply, operation of cooling system, and laser alignment. Students also acquire an appreciation of the arts, learning about shapes and contours as they develop graphics for the shows. After holography, laser show animation provides a combination of the arts and technology. This paper aims to briefly describe how a krypton-argon laser, galvanometer scanners, a polychromatic acousto-optic modulator and related electronics are put together to develop a laser projector. The paper also describes how students are trained to make their own laser animation and beam effects with music, and at the same time have an appreciation of the operation of a Class IV laser and the handling of optical components.

  20. See you at London Vet Show.

    Science.gov (United States)

    2016-11-05

    London Vet Show is fast approaching: it takes place from November 17 to 18 and is being held at ExCeL London for the first time. Zoe Davies, marketing manager, highlights some of what BVA is offering at the event. British Veterinary Association.

  1. UV Photography Shows Hidden Sun Damage

    Science.gov (United States)

    ... var c = 0; c UV photography shows hidden sun damage A UV photograph gives us a safe way to see how the sun damages our skin. In the UV photos that ... on the right, you can see what hidden sun damage looks like. Compare these UV photos with ...

  2. Effects of lifestyle intervention in pregnancy and anthropometrics at birth on offspring metabolic profile at 2.8 years - results from the Lifestyle in Pregnancy and Offspring (LiPO) study

    DEFF Research Database (Denmark)

    Tanvig, Mette; Vinter, Christina A; Jørgensen, Jan S

    2014-01-01

    Context: Maternal obesity and gestational weight gain are linked to offspring adverse metabolic profile, and lifestyle intervention during pregnancy in obese women may have long-term positive effect on their children. Furthermore, although the association between birth weight and later metabolic...... outcomes is well established, little is known about the predictive value of abdominal circumference at birth. Objectives: To study: i) effects of lifestyle intervention during pregnancy in obese women on offspring metabolic risk factors and ii) predictive values of birth weight (BW) and birth abdominal...... circumference (BAC). Design: Follow-up of a randomized controlled trial; the Lifestyle in Pregnancy (LiP) study Setting: Odense and Aarhus University Hospitals, Denmark Participants: Offspring of LiP study participants (n=157) and offspring of normal weight mothers (external reference group, ER, n=97...

  3. Long-term Results from the Empowering a Multimodal Pathway Toward Healthy Youth Program, a Multimodal School-Based Approach, Show Marked Reductions in Suicidality, Depression, and Anxiety in 6,227 Students in Grades 6–12 (Aged 11–18)

    Science.gov (United States)

    Silverstone, Peter H.; Bercov, Marni; Suen, Victoria Y. M.; Allen, Andrea; Cribben, Ivor; Goodrick, Jodi; Henry, Stu; Pryce, Catherine; Langstraat, Pieter; Rittenbach, Katherine; Chakraborty, Samprita; Engles, Rutger C.; McCabe, Christopher

    2017-01-01

    Here, we report on findings from a 15-month follow-up of a school-based program called Empowering a Multimodal Pathway Toward Healthy Youth (EMPATHY). This was primarily intended to reduce suicidal thinking in pre-teens, adolescents, and youth students aged 11–18 in middle schools (Grades 6–8) and high SCHOOLS (Grades 9–12). It also aimed to reduce depression and anxiety. The EMPATHY multimodal program consisted of repeated data collection, identification of a high-risk group, a rapid intervention for this high-risk group including offering supervised online cognitive behavioral therapy (CBT) program, a universal CBT intervention for those in Grades 6–8, a variety of interactions with trained staff (“Resiliency Coaches”), and referral to external medical and psychiatric services where appropriate. There were four time-points at which assessments were made: baseline, 3, 7, and 15 months. Here, we report cross-sectional findings over 15 months in a total of 6,227 students who were assessed at least once during the study period. Additionally, we report longitudinal findings from the 1,884 students who completed all 4 assessments. Our results found highly statistically significant decreases in suicidality rates, with the percentage of the total school population who were actively suicidal decreasing from 4.4% at baseline (n = 143 of 3,244) to 2.8% at 15 months (n = 125 of 4,496) (p anxiety scores at each time-point. Thus, Mean Depression scores at baseline for the entire student population were 3.73 ± 3.87 (n = 3,244) at baseline and decreased to 3.22 ± 3.52 (n = 4,496) (p anxiety scores at all time-points. For example, depression scores decreased from a mean of 3.43 ± 3.67 (n = 1,884) at baseline to 2.95 ± 3.53 (n = 1,884) at 15-months (p school-based multimodal programs, utilizing a combination of interventions, can have meaningful benefits across entire school populations. PMID:28555115

  4. Technical player profiles related to the physical fitness of young female volleyball players predict team performance.

    Science.gov (United States)

    Dávila-Romero, C; Hernández-Mocholí, M A; García-Hermoso, A

    2015-03-01

    This study is divided into three sequential stages: identification of fitness and game performance profiles (individual player performance), an assessment of the relationship between these profiles, and an assessment of the relationship between individual player profiles and team performance during play (in championship performance). The overall study sample comprised 525 (19 teams) female volleyball players aged 12-16 years and a subsample (N.=43) used to examine study aims one and two was selected from overall sample. Anthropometric, fitness and individual player performance (actual game) data were collected in the subsample. These data were analyzed through clustering methods, ANOVA and independence chi-square test. Then, we investigated whether the proportion of players with the highest individual player performance profile might predict a team's results in the championship. Cluster analysis identified three volleyball fitness profiles (high, medium, and low) and two individual player performance profiles (high and low). The results showed a relationship between both types of profile (fitness and individual player performance). Then, linear regression revealed a moderate relationship between the number of players with a high volleyball fitness profile and a team's results in the championship (R2=0.23). The current study findings may enable coaches and trainers to manage training programs more efficiently in order to obtain tailor-made training, identify volleyball-specific physical fitness training requirements and reach better results during competitions.

  5. Optimized Estimation of Surface Layer Characteristics from Profiling Measurements

    Directory of Open Access Journals (Sweden)

    Doreene Kang

    2016-01-01

    Full Text Available New sampling techniques such as tethered-balloon-based measurements or small unmanned aerial vehicles are capable of providing multiple profiles of the Marine Atmospheric Surface Layer (MASL in a short time period. It is desirable to obtain surface fluxes from these measurements, especially when direct flux measurements are difficult to obtain. The profiling data is different from the traditional mean profiles obtained at two or more fixed levels in the surface layer from which surface fluxes of momentum, sensible heat, and latent heat are derived based on Monin-Obukhov Similarity Theory (MOST. This research develops an improved method to derive surface fluxes and the corresponding MASL mean profiles of wind, temperature, and humidity with a least-squares optimization method using the profiling measurements. This approach allows the use of all available independent data. We use a weighted cost function based on the framework of MOST with the cost being optimized using a quasi-Newton method. This approach was applied to seven sets of data collected from the Monterey Bay. The derived fluxes and mean profiles show reasonable results. An empirical bias analysis is conducted using 1000 synthetic datasets to evaluate the robustness of the method.

  6. Project Listen Compute Show (LCS) - Marine

    Science.gov (United States)

    2004-02-01

    TECHNICAL REPORT NATICK/TR-04/010 APA ^QO^U PROJECT LISTEN, COMPUTE, SHOW (LCS) - MARINE by Victor Zue* John Wroclawski* and Michael Bolotski...Voice Communication Strategy 37 6.3 Adaptive Channel Multiplexing 38 6.4 Web Performance and the Cache Information Protocol 41 7. Handheld Device...view of the BB-4 stack. The digital board is at the top. Most of the area is the large FPGA and the two frame buffer memories 61 Figure 14

  7. Trematode Hemoglobins Show Exceptionally High Oxygen Affinity

    OpenAIRE

    Kiger, Laurent; Rashid, Aftab K.; Griffon, Nathalie; Haque, Masoodul; Moens, Luc; Gibson, Quentin H.; Poyart, Claude; Marden, Michael C.

    1998-01-01

    Ligand binding studies were made with hemoglobin (Hb) isolated from trematode species Gastrothylax crumenifer (Gc), Paramphistomum epiclitum (Pe), Explanatum explanatum (Ee), parasitic worms of water buffalo Bubalus bubalis, and Isoparorchis hypselobagri (Ih) parasitic in the catfish Wallago attu. The kinetics of oxygen and carbon monoxide binding show very fast association rates. Whereas oxygen can be displaced on a millisecond time scale from human Hb at 25 degrees C, the dissociation of ox...

  8. The influence of humidity fluxes on offshore wind speed profiles

    Directory of Open Access Journals (Sweden)

    R. J. Barthelmie

    2010-05-01

    Full Text Available Wind energy developments offshore focus on larger turbines to keep the relative cost of the foundation per MW of installed capacity low. Hence typical wind turbine hub-heights are extending to 100 m and potentially beyond. However, measurements to these heights are not usually available, requiring extrapolation from lower measurements. With humid conditions and low mechanical turbulence offshore, deviations from the traditional logarithmic wind speed profile become significant and stability corrections are required. This research focuses on quantifying the effect of humidity fluxes on stability corrected wind speed profiles. The effect on wind speed profiles is found to be important in stable conditions where including humidity fluxes forces conditions towards neutral. Our results show that excluding humidity fluxes leads to average predicted wind speeds at 150 m from 10 m which are up to 4% higher than if humidity fluxes are included, and the results are not very sensitive to the method selected to estimate humidity fluxes.

  9. Reality, ficción o show

    Directory of Open Access Journals (Sweden)

    Sandra Ruíz Moreno

    2002-01-01

    Full Text Available Para tener un punto de vista claro y objetivo frente a la polémica establecida en torno al programa “Protagonistas de novela” y la tendiente proliferación de los reality show en las parrillas de programación de la televisión colombiana, se realizó un análisis de texto y contenido de dicho programa, intentando definirlo desde sus posibilidades de realidad, ficción y show. Las unidades de análisis y el estudio de su tratamiento arrojaron un alto contenido que gira en torno a las emociones del ser humano relacionadas con la convivencia, tratadas a manera de show y con algunos aportes textuales de ficción, pero sin su elemento mediador básico, el actor, quitándole toda la posibilidad de tener un tratamiento con la profundidad, distancia y ética que requieren los temas de esta índole. El resultado es un formato que sólo busca altos índices de sintonía y que pertenece más a la denominada televisión “trash”, que a una búsqueda de realidad del hombre y mucho menos de sociedad.

  10. Measurement of electron density profile by microwave reflectometry on tokamaks

    Energy Technology Data Exchange (ETDEWEB)

    Simonet, F.

    1985-05-01

    A new method for measuring the electron density spatial profile has been successfully tested on the tokamak of Fontenay aux Roses (TFR). This method is based on the total reflection experienced by a wave of frequency F on the layer where F = F/sub p/e(r). The experimental results show that the maximum electron density in the discharge is also easily measured and that accurate determination of a density profile can be obtained with a time resolution of 5 ms. This diagnostic is well adapted to all fusion devices where access to the total plasma cross section is limited, particularly for large tokamaks.

  11. Soil Moisture Profile Effect on Radar Signal Measurement

    OpenAIRE

    André Chanzy; Nicolas Baghdadi; Mehrez Zribi; Aurélie Le Morvan

    2008-01-01

    The objective of this paper is to analyze the behaviour of a backscattered signal according to soil moisture depth over bare soils. Analysis based on experimental vertical moisture profiles and ASAR/ENVISAT measurements has been carried out. A modified IEM model with three permittivity layers (0-1cm, 1-2cm, 2-5cm) has been developed and used in this study. Results show a small effect of moisture profile on the backscattered signal (less than 0.5dB). However, measurements and simulations have ...

  12. Software for portable laser light show system

    Science.gov (United States)

    Buruchin, Dmitrey J.; Leonov, Alexander F.

    1995-04-01

    Portable laser light show system LS-3500-10M is connected to the parallel port of IBM PC/AT compatible computer. Computer performs output of digital control data describing images. Specially designed control device is used to convert digital data coming from parallel port to the analog signal driving scanner. Capabilities of even cost nothing 286 computer are quite enough for laser graphics control. Technology of scanning used in laser graphics system LS-3500-10M essentially differs from widely spread systems based on galvanometers with mobile core or with mobile magnet. Such devices are based on the same principle of work as electrically driven servo-mechanism. As scanner we use elastic system with hydraulic dampen oscillations and opened loop. For most of applications of laser graphics such system provides satisfactory precision and speed of scanning. LS-3500-10M software gives user ability to create on PC and play his own laser graphics demonstrations. It is possible to render recognizable text and pictures using different styles, 3D and abstract animation. All types of demonstrations can be mixed in slide-show. Time synchronization is supported. Software has the following features: (1) Different types of text output. Built-in text editor for typing and editing of textural information. Different fonts can be used to display text. User can create his own fonts using specially developed font editor. (2) Editor of 3D animation with library of predefined shapes. (3) Abstract animation provided by software routines. (4) Support of different graphics files formats (PCX or DXF). Original algorithm of raster image tracing was implemented. (5) Built-in slide-show editor.

  13. Perfil do idoso em área metropolitana na região sudeste do Brasil: resultados de inquérito domiciliar Profile of the elderly in an metropolitan area of Southeastern Brazil: results of a household survey

    Directory of Open Access Journals (Sweden)

    Luiz Roberto Ramos

    1993-04-01

    Full Text Available Trata-se de estudo multicêntrico visando levantar as necessidades de saúde da população de idosos residentes em zona urbana, conduzido em 6 países na América Latina e coordenado pela Organização Panamericana da Saúde. No Brasil, 1.602 idosos (60 anos e + residentes no Distrito de São Paulo, participaram de inquérito domiciliar com questionário de avaliação funcional multidimensional - amostra populacional aleatória, em múltiplos estágios, estratificada por nível socioeconômico. Os resultados mostraram uma população bastante carente (70% tinha uma renda per capita de menos de 100 dólares por mês, vivendo predominantemente em domicílios multigeracionais (59% viviam com os filhos e/ou com netos, com alta prevalência de doenças crônicas (somente 14% referiu não ter nenhuma doença e distúrbios psiquiátricos (27% foram considerados casos psiquiátricos, e com uma elevada proporção de pessoas com perda de autonomia (47% precisavam de ajuda para realizar pelo menos uma das atividades da vida diária. Os resultados são analisados tendo em vista as demandas futuras por serviços de saúde especializados e suporte social por parte da crescente população de idosos no Brasil.A multicentre study concerned with the health needs of the elderly population living in urban areas and coordinated by the Panamerican Health Organization was undertaken in 6 Latin-American countries. In Brazil, 1,602 elderly residents (of 60 years of age and over in the District of S. Paulo, constituting a multistage random sample stratified by socioeconomic status, participated in a household survey using a multidemensional functional assessment questionnaire. The results showed a highly deprived population (70% had a per capita income of less than US$ 100 per month, living mostly in multigenerational households (59% were living with children and/or grandchildren, with a high prevalence of chronic physical illnesses (only 14% referred no illness and

  14. Ozone ProfilE Retrieval Algorithm (OPERA for nadir-looking satellite instruments in the UV–VIS

    Directory of Open Access Journals (Sweden)

    J. C. A. van Peet

    2014-03-01

    Full Text Available For the retrieval of the vertical distribution of ozone in the atmosphere the Ozone ProfilE Retrieval Algorithm (OPERA has been further developed. The new version (1.26 of OPERA is capable of retrieving ozone profiles from UV–VIS observations of most nadir-looking satellite instruments like GOME, SCIAMACHY, OMI and GOME-2. The setup of OPERA is described and results are presented for GOME and GOME-2 observations. The retrieved ozone profiles are globally compared to ozone sondes for the years 1997 and 2008. Relative differences between GOME/GOME-2 and ozone sondes are within the limits as specified by the user requirements from the Climate Change Initiative (CCI programme of ESA (20% in the troposphere, 15% in the stratosphere. To demonstrate the performance of the algorithm under extreme circumstances, the 2009 Antarctic ozone hole season was investigated in more detail using GOME-2 ozone profiles and lidar data, which showed an unusual persistence of the vortex over the Río Gallegos observing station (51° S, 69.3° W. By applying OPERA to multiple instruments, a time series of ozone profiles from 1996 to 2013 from a single robust algorithm can be created.

  15. Asteroid Ida - 6 Views Showing Rotation

    Science.gov (United States)

    1994-01-01

    This composite image shows the asteroid 243 Ida as seen from the Galileo spacecraft during its approach on August 28, 1993. The six views were shuttered through the camera's green filter and show Ida's rotation over a period of about 3 hours 18 minutes. The asteroid makes a complete rotation every 4 hours 38 minutes; therefore, this set of images spans about 3/4 of Ida's rotation period and shows most of Ida's surface. By combining the information in these views with that from the highest resolution images returned from the spacecraft in September 1993, the size and shape of this irregular body can now be determined accurately The asteroid appears to be about 58 kilometers (36 miles) long and about 23 kilometers wide, with a very irregular shape and volume of some 16,000 cubic kilometers. The images are arranged in chronological order from a time 3 hours 51 minutes before closest approach (upper left), through upper right, middle left, middle right lower left and lower right (33 minutes before closest approach). The six images show Ida at the same scale throughout. Ida's rotation axis is roughly vertical in these images, and the rotation causes the right-hand end of Ida to move toward the viewer as time progresses. The first image was taken from a range of about 171,000 km (106,000 miles) and provides an image resolution of about 1,700 meters per pixel (the highest resolution achieved for Ida is about 25 meters per pixel). The second, taken 70 minutes later, is from 119,000 kilometers, followed by 102,000 kilometers, 85,000 kilometers, 50,000 kilometers, and 25,000 kilometers. The features on Ida are less sharp in the earlier views because of the greater distances. Prominent in the middle three views is a deep depression across the short axis of the Asteroid. This feature tends to support the idea that Ida may have originally been formed from two or more separate large objects that collided softly and stuck together. Also visible in the lower left view is an

  16. Tomato Fruits Show Wide Phenomic Diversity but Fruit Developmental Genes Show Low Genomic Diversity.

    Directory of Open Access Journals (Sweden)

    Vijee Mohan

    Full Text Available Domestication of tomato has resulted in large diversity in fruit phenotypes. An intensive phenotyping of 127 tomato accessions from 20 countries revealed extensive morphological diversity in fruit traits. The diversity in fruit traits clustered the accessions into nine classes and identified certain promising lines having desirable traits pertaining to total soluble salts (TSS, carotenoids, ripening index, weight and shape. Factor analysis of the morphometric data from Tomato Analyzer showed that the fruit shape is a complex trait shared by several factors. The 100% variance between round and flat fruit shapes was explained by one discriminant function having a canonical correlation of 0.874 by stepwise discriminant analysis. A set of 10 genes (ACS2, COP1, CYC-B, RIN, MSH2, NAC-NOR, PHOT1, PHYA, PHYB and PSY1 involved in various plant developmental processes were screened for SNP polymorphism by EcoTILLING. The genetic diversity in these genes revealed a total of 36 non-synonymous and 18 synonymous changes leading to the identification of 28 haplotypes. The average frequency of polymorphism across the genes was 0.038/Kb. Significant negative Tajima'D statistic in two of the genes, ACS2 and PHOT1 indicated the presence of rare alleles in low frequency. Our study indicates that while there is low polymorphic diversity in the genes regulating plant development, the population shows wider phenotype diversity. Nonetheless, morphological and genetic diversity of the present collection can be further exploited as potential resources in future.

  17. Are Corporate Universities (CU possible in emerging countries? A survey conducted in Argentina showed impacting results

    Directory of Open Access Journals (Sweden)

    Leandro A. Viltard

    2014-09-01

    Full Text Available At the time of our investigation, the CU was not a widespread concept in Argentina, being viewed as a “foreign - far long project” (coming from developed countries and standing for the long term. It is suggested that the rate of CU evolution, in emerging countries like Argentina, is more related to mentality issues than to CU strategic or operative limitations. Although the executives who replied to a survey were not the only power factor in their organization, their comments allow us to think that, in those countries, the CU may have a better future perspective. The research used a quali-quantitative methodology, which was based on a survey to top executives of different kinds of companies located in Argentina. The research design was not experimental and transversal, as it was limited to a specific moment in time.

  18. Pilot evaluation of a media literacy program for tobacco prevention targeting early adolescents shows mixed results.

    Science.gov (United States)

    Kaestle, Christine E; Chen, Yvonnes; Estabrooks, Paul A; Zoellner, Jamie; Bigby, Brandon

    2013-01-01

    The purpose of this pilot study was to assess the impact of media literacy for tobacco prevention for youth delivered through a community site. A randomized pretest-posttest evaluation design with matched-contact treatment and control conditions. The pilot study was delivered through the YMCA in a lower-income suburban and rural area of Southwest Virginia, a region long tied, both economically and culturally, to the tobacco industry. Children ages 8 to 14 (76% white, 58% female) participated in the study (n = 38). The intervention was an antismoking media literacy program (five 1-hour lessons) compared with a matched-contact creative writing control program. General media literacy, three domains of tobacco-specific media literacy ("authors and audiences," "messages and meanings," and "representation and reality"), tobacco attitudes, and future expectations were assessed. Multiple regression modeling assessed the impact of the intervention, controlling for pretest measures, age, and sex. General media literacy and tobacco-specific "authors and audiences" media literacy improved significantly for treatment compared with control (p literacy measures and for tobacco attitudes were not significant. Future expectations of smoking increased significantly for treatment participants ages 10 and younger (p literacy are accompanied by an increase in future expectations to smoke for younger children.

  19. FES Training in Aging: interim results show statistically significant improvements in mobility and muscle fiber size

    OpenAIRE

    Helmut Kern; Stefan Loefler; Christian Hofer; Michael Vogelauer; Samantha Burggraf; Martina Grim-Stieger; Jan Cvecka; Dusan Hamar; Nejc Sarabon; Feliciano Protasi; Antonio Musarò; Marco Sandri; Katia Rossini; Ugo Carraro; Sandra Zampieri

    2012-01-01

    Aging is a multifactorial process that is characterized by decline in muscle mass and performance. Several factors, including reduced exercise, poor nutrition and modified hormonal metabolism, are responsible for changes in the rates of protein synthesis and degradation that drive skeletal muscle mass reduction with a consequent decline of force generation and mobility functional performances. Seniors with normal life style were enrolled: two groups in Vienna (n=32) and two groups in Bratisla...

  20. Cacodylic acid for precommercial thinning in mixed-conifer stands shows erratic results.

    Science.gov (United States)

    William W. Oliver

    1970-01-01

    In a small-scale test, a silvicide consisting of cacodylic acid was injected during the growing season at dosages recommended by the manufacturer. The treatment did not thin adequately two of three mixed-conifer stands. Ponderosa pine and lower crown classes seemed more susceptible to the silvicide than Douglas-fir and upper crown classes. No flashback was recognized....

  1. EXPERT SYSTEMS SHOW PROMISE FOR CUSTOMER INQUIRIES

    Science.gov (United States)

    This article describes results of an agreement between the North Penn Water Authority in Lansdale, Pa., and the US Environmental Protection Agency, Drinking Water Research Division, Cincinnati, Ohio, to study use of expert systems technology in a water utility. The threeyear stud...

  2. Reports show fewer pallets entering landfills

    Science.gov (United States)

    Robert J. Bush; Philip A. Araman

    2010-01-01

    Information from a series of Virginia Tech studies reveals current trends in core utilization by the pallet industry. The most recent report released this year studied trends from 2006. Virginia Tech researchers compared these results to five previous studies going all the way back to 1992.

  3. GLL RPT IONOSPHERE PROFILES

    Data.gov (United States)

    National Aeronautics and Space Administration — The Galileo Radio Propagation Team Ionosphere Profile data set is small number of electron density profiles derived from radio occultation data collected while...

  4. GHGRP Industrial Profiles

    Science.gov (United States)

    EPA's Greenhouse Gas Reporting Program periodically produces detailed profiles of the various industries that report under the program. These profiles contain detailed analyses. This page hosts data highlights for all sectors.

  5. HOPWA Performance Profiles

    Data.gov (United States)

    Department of Housing and Urban Development — HOPWA Performance Profiles are generated quarterly for all agencies receiving HOPWA formula or competitive grants. Performance Profiles are available at the national...

  6. Bully/Victim Profiles' Differential Risk for Worsening Peer Acceptance: The Role of Friendship.

    Science.gov (United States)

    Kochel, Karen P; Ladd, Gary W; Bagwell, Catherine L; Yabko, Brandon A

    2015-01-01

    Study aims were to: (1) evaluate the association between bully/victim profiles, derived via latent profile analysis (LPA), and changes in peer acceptance from the fall to spring of 7 th grade, and (2) investigate the likelihood of friendlessness, and the protective function of mutual friendship, among identified profiles. Participants were 2,587 7 th graders; peer nomination and rating-scale data were collected in the fall and spring. Four profiles, including bullies, victims, bully-victims, and uninvolved adolescents, were identified at each time point. Findings showed that for victims, more so than for bullies and uninvolved profiles, acceptance scores worsened over time. Results further revealed that bully-victim and victim profiles included a greater proportion of friendless youth relative to the bully profile, which, in turn, contained a greater proportion of friendless adolescents than the uninvolved profile. Findings also provided evidence for the buffering role of friendship among all bully/victim profiles and among bully-victims especially.

  7. Microbiological and environmental issues in show caves.

    Science.gov (United States)

    Saiz-Jimenez, Cesareo

    2012-07-01

    Cultural tourism expanded in the last half of the twentieth century, and the interest of visitors has come to include caves containing archaeological remains. Some show caves attracted mass tourism, and economical interests prevailed over conservation, which led to a deterioration of the subterranean environment and the rock art. The presence and the role of microorganisms in caves is a topic that is often ignored in cave management. Knowledge of the colonisation patterns, the dispersion mechanisms, and the effect on human health and, when present, over rock art paintings of these microorganisms is of the utmost importance. In this review the most recent advances in the study of microorganisms in caves are presented, together with the environmental implications of the findings.

  8. PROFILER: 1D galaxy light profile decomposition

    Science.gov (United States)

    Ciambur, Bogdan C.

    2017-05-01

    Written in Python, PROFILER analyzes the radial surface brightness profiles of galaxies. It accurately models a wide range of galaxies and galaxy components, such as elliptical galaxies, the bulges of spiral and lenticular galaxies, nuclear sources, discs, bars, rings, and spiral arms with a variety of parametric functions routinely employed in the field (Sérsic, core-Sérsic, exponential, Gaussian, Moffat and Ferrers). In addition, Profiler can employ the broken exponential model (relevant for disc truncations or antitruncations) and two special cases of the edge-on disc model: namely along the major axis (in the disc plane) and along the minor axis (perpendicular to the disc plane).

  9. Genotypically defined lissencephalies show distinct pathologies.

    Science.gov (United States)

    Forman, Mark S; Squier, Waney; Dobyns, William B; Golden, Jeffrey A

    2005-10-01

    Lissencephaly is traditionally divided into 2 distinct pathologic forms: classic (type I) and cobblestone (type II). To date, mutations in 4 genes, LIS1, DCX, RELN, and ARX, have been associated with distinct type I lissencephaly syndromes. Each of these genes has been shown to play a role in normal cell migration, consistent with the presumed pathogenesis of type I lissencephaly. Based on these data, we hypothesized that all forms of radiographically defined type I lissencephaly independent of genotype would be pathologically similar. To test this hypothesis, we examined brains from 16 patients, including 15 lissencephalic patients and one patient with subcortical band heterotopia. Of these 16 patients, 6 had LIS1 deletions, 2 had DCX mutations, and 2 had ARX mutations. In addition, 6 patients had no defined genetic defect, although the patient with subcortical band heterotopia exhibited the same pattern of malformation expected with an XLIS mutation. In all cases, the cortex was thickened; however, the topographic distribution of the cortical pathology varied, ranging from frontal- to occipital-biased pathology to diffuse involvement of the neocortex. Although brains with LIS1 deletions exhibited the classic 4-layer lissencephalic architecture, patients with DCX and ARX mutations each had unique cytoarchitectural findings distinct from LIS1. Furthermore, 2 of the 5 patients with no known genetic defect showed a fourth type of histopathology characterized by a 2-layered cortex. Interestingly, the 2 brains with the fourth type of lissencephaly showed profound brainstem and cerebellar abnormalities. In summary, we identified at least 4 distinct histopathologic subtypes of lissencephaly that stratify with the underlying genetic defect. Based on these data, a new classification for lissencephaly is proposed that incorporates both pathologic and genetic findings.

  10. Relative vegetation profiles in a Neotropical forest: comparison of lidar instrumentation and field-based measurements

    Science.gov (United States)

    Sullivan, F. B.; Palace, M. W.; Ducey, M.; Czarnecki, C.; Zanin Shimbo, J.; Mota e Silva, J.

    2012-12-01

    Tropical forests are considered to be some of the most structurally complex forests in the world. Understanding vegetation height structure in these forests can aid in understanding the spatial temporal components of disturbance, from blowdowns to gap dynamics. Vegetation profiles can be used to better estimate carbon storage and flux across the landscape. Using light detection and ranging (lidar) data collected at La Selva, Costa Rica from four instruments (three airborne, one terrestrial) at four times since 2005, and field data collected in January 2012, we generated relative vegetation profiles for twenty plots in La Selva. Relative vegetation profiles were derived from lidar data by accounting for obscured plant material through a log transformation of the cumulative proportion of observations (percent canopy closure). Profiles were derived from field data using two different sets of allometric equations describing crown shape and tree height. We conducted a cluster analysis on similarity matrices developed in R (version 2.14.1) using three different metrics (sum of squares, Kullback-Leibler divergence, Kolmogorov-Smirnov D statistic) and identified general similarity between lidar profiles. Results were consistent across each of the three similarity metrics. Three distinct clusters were found, with profiles from three airborne lidar instruments, two profiles from a terrestrial lidar instrument, and profiles derived from field data forming the clusters. Our results indicate that although estimating lidar relative vegetation profiles from field data was not possible, terrestrial lidar relative vegetation profiles are generally similar to airborne relative vegetation profiles. Given the rapidity and repeatability of terrestrial lidar measurements, these results show promise for terrestrial lidar instruments to collect plot-specific data on forest structure and vertical distribution of plant material. Furthermore, identifying relationships between terrestrial and

  11. Individual ant workers show self-control.

    Science.gov (United States)

    Wendt, Stephanie; Czaczkes, Tomer J

    2017-10-01

    Often, the first option is not the best. Self-control can allow humans and animals to improve resource intake under such conditions. Self-control in animals is often investigated using intertemporal choice tasks-choosing a smaller reward immediately or a larger reward after a delay. However, intertemporal choice tasks may underestimate self-control, as test subjects may not fully understand the task. Vertebrates show much greater apparent self-control in more natural foraging contexts and spatial discounting tasks than in intertemporal choice tasks. However, little is still known about self-control in invertebrates. Here, we investigate self-control in the black garden ant Lasius niger We confront individual workers with a spatial discounting task, offering a high-quality reward far from the nest and a poor-quality reward closer to the nest. Most ants (69%) successfully ignored the closer, poorer reward in favour of the further, better one. However, when both the far and the close rewards were of the same quality, most ants (83%) chose the closer feeder, indicating that the ants were indeed exercising self-control, as opposed to a fixation on an already known food source. © 2017 The Author(s).

  12. Quantity language speakers show enhanced subcortical processing.

    Science.gov (United States)

    Dawson, Caitlin; Aalto, Daniel; Šimko, Juraj; Putkinen, Vesa; Tervaniemi, Mari; Vainio, Martti

    2016-07-01

    The complex auditory brainstem response (cABR) can reflect language-based plasticity in subcortical stages of auditory processing. It is sensitive to differences between language groups as well as stimulus properties, e.g. intensity or frequency. It is also sensitive to the synchronicity of the neural population stimulated by sound, which results in increased amplitude of wave V. Finnish is a full-fledged quantity language, in which word meaning is dependent upon duration of the vowels and consonants. Previous studies have shown that Finnish speakers have enhanced behavioural sound duration discrimination ability and larger cortical mismatch negativity (MMN) to duration change compared to German and French speakers. The next step is to find out whether these enhanced duration discrimination abilities of quantity language speakers originate at the brainstem level. Since German has a complementary quantity contrast which restricts the possible patterns of short and long vowels and consonants, the current experiment compared cABR between nonmusician Finnish and German native speakers using seven short complex stimuli. Finnish speakers had a larger cABR peak amplitude than German speakers, while the peak onset latency was only affected by stimulus intensity and spectral band. The results suggest that early cABR responses are better synchronised for Finns, which could underpin the enhanced duration sensitivity of quantity language speakers. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  13. Effects of lifestyle intervention in pregnancy and anthropometrics at birth on offspring metabolic profile at 2.8 years: results from the Lifestyle in Pregnancy and Offspring (LiPO) study.

    Science.gov (United States)

    Tanvig, Mette; Vinter, Christina A; Jørgensen, Jan S; Wehberg, Sonja; Ovesen, Per G; Beck-Nielsen, Henning; Christesen, Henrik T; Jensen, Dorte M

    2015-01-01

    Maternal obesity and gestational weight gain are linked to offspring adverse metabolic profiles, and lifestyle interventions during pregnancy in obese women may have long-term positive effects on their children. Furthermore, although the association between birth weight and later metabolic outcomes is well established, little is known about the predictive value of abdominal circumference at birth. The purpose of this study was to determine (1) the effects of lifestyle interventions during pregnancy in obese women on offspring metabolic risk factors and (2) predictive values of birth weight (BW) and birth abdominal circumference (BAC). This was a follow-up of a randomized controlled trial, the Lifestyle in Pregnancy (LiP) study. The study was conducted in Odense and Aarhus University Hospitals, Denmark. We studied the offspring of LiP study participants (n = 157) and offspring of normal-weight mothers (external reference group, n = 97). INTERVENTIONs included dietary advice, coaching, and exercise during pregnancy. The outcome measures were body mass index (BMI) Z-score, abdominal circumference, blood pressure, and fasting plasma glucose, insulin, high-density lipoprotein, and triglycerides at the age of 2.8 years. No differences were detected in BMI Z-scores or metabolic risk factors between the LiP intervention and control groups or between the LiP and external reference groups. BAC and BW were associated (all P lifestyle interventions in obese pregnant women. Offspring of obese mothers who participated in the LiP study were comparable to offspring of normal-weight mothers, possibly indicating a general beneficial effect of trial participation. BAC and BW were both associated with later metabolic risk factors.

  14. An equilibrium profile model for tidal environments

    Directory of Open Access Journals (Sweden)

    A. M. Bernabeu

    2002-12-01

    Full Text Available During a full tidal cycle, the beach profile is exposed to continuously changing hydrodynamical conditions. Consequently, the profile evolves constantly to adapt to these changes. The equilibrium condition on tidal beaches is defined in terms of the relative occurrence of swash, surf zone and shoaling processes. We have assumed that the tidal beach profile is in equilibrium when the net sediment transport along a tidal cycle is zero. In this model the contribution of swash is considered negligible. A simple and easy-to-apply equilibrium profile formulation is proposed. This model is based on the assumption that surf zone processes dominate the profile morphology wherever wave breaking occurs during the tidal cycle. The obtained equilibrium profile is valid from the high tide level to the breaker point at low tide level. The tidal influence on the profile morphology is the lengthening of the surf profile. The higher the tidal range, the longer the surf profile. The model was tested against field and laboratory data, showing reasonable predictions of measured beach profiles.

  15. Spatial resolution limits of an optical profiler

    Science.gov (United States)

    Creath, Katherine

    1990-07-01

    Interferometric optical profilers have a spatial resolution which is either limited by the detector array sample spacing and element size or by the optical resolution of the system. To test the working spatial resolution of an optical profiler a sinsusoidal grating with 300 lines/mm was measured using an optical profiler at lOx 2Ox 40x and 200x with detector arrays having element-to-element spacings of 6. 8 j. tm and 40 tm. The highest magnification gave the greatest and most accurate depth for the grating for all of the detectors. At 40x as long as there were more than about 8 sample points per cycle as there were with the two smaller detector spacings the grating depth can be measured quite accurately. With fewer points the peak-to-valley height measurement of the grating is too low even though the optical resolution of the system is sufficient enough to resolve the grating. The results of this work show that for accurate representation of surface heights containing high frequency structures oversampling is desirable. Summary The spatial resolution of an interferometric optical proffler depends upon both the optical resolution of the system and the characteristics of the detector array used to sample the image. The limiting resolution wifi be the larger of the optical and detector resolution. One means of defining optical resolution is the Sparrow criterion which states that the image of two points is just

  16. The safety profile of the fluoroquinolones.

    Science.gov (United States)

    Bertino, J; Fish, D

    2000-07-01

    Premarketing trials showed the fluoroquinolone agents to have a favorable side-effect profile, with treatment-related adverse events comprising gastrointestinal, central nervous system, and dermatologic effects that were generally mild and reversible on cessation of treatment. However, postmarketing surveillance studies have identified severe adverse events, including severe anaphylaxis, QTc-interval prolongation, and potential cardiotoxicity, associated with 3 quinolone agents that either resulted in the removal of the agent from the market (temafloxacin and grepafloxacin) or significantly restricted its use due to substantial mortality and morbidity associated with liver toxicity (trovafloxacin). To date, there have been no such significant adverse events associated with the older fluoroquinolone agents, including ciprofloxacin, ofloxacin, norfloxacin, and levofloxacin. However, there are fewer data from postmarketing surveillance studies on the most recently approved agents, such as moxifloxacin and gatifloxacin, or agents awaiting approval, such as gemifloxacin. This paper examines safety data from the premarketing trials and postmarketing surveillance studies of fluoroquinolones available in the United States. A MEDLINE search was performed to identify all English-language studies published since 1980 concerning the safety profiles of the fluoroquinolones. The fluoroquinolone antibacterial agents offer broad-spectrum therapy in patients with a variety of infections. Given similar spectra of activity, the choice between quinolones may be based on differences in efficacy and safety or tolerability profiles. Most drug reactions involving these agents are minor and reversible on discontinuing treatment, but adverse effects can be associated with significant mortality and morbidity, as was seen in the case of trovafloxacin and temafloxacin.

  17. Characteristics between the meshing pairs with different envelope profile in single screw compressors

    Science.gov (United States)

    Huang, R.; Liu, F.; Li, T.; Feng, Q.

    2017-08-01

    Single screw compressors have been used in various industrial fields. However, because the star-wheel teeth are easy to wear, the market for the development of single screw compressors is limited. In order to extend the service life of the star-wheel, researchers have developed different kinds of star-wheel tooth profile, such as single line envelope profile, single column envelope profile, and multi-column envelope profile. These profiles greatly affect the lubrication characteristics between the star-wheel teeth and the screw grooves. In this article, the lubrication characteristics between the meshing pairs with different envelope profiles are analyzed. Results show that the pressure peak of the single line envelope profile, single column envelope profile, and multi-column envelope profile are 3.23×105Pa, 3.38×105Pa, and 4.31×105Pa, respectively. This means that the multi-column enveloped meshing pair can resist the biggest external impact load. The deviation angle (γ) of the single line envelope profile, single column envelope profile, and multi-column envelope profile are 0.0139°~0.0286°, 0.0225°~0.0306° and 0.0122°~0.0262°, respectively. Thus, the self-balancing ability of the multi-column enveloped meshing pair is the strongest, and the oil film thickness on both sides of the multi-column enveloped star-wheel tooth is the most reasonable, which indicates a good lubrication state during operation, that is, longer operation life of the star-wheel teeth.

  18. Offshore technology stresses substance over show

    Energy Technology Data Exchange (ETDEWEB)

    Wright, T.R. Jr.; Abraham, K.S.

    1988-07-01

    Emphasis these days continues to be on practical, cost-effective solutions to real problems. With a couple of exceptions, gone are the glitz and glamour of big-ticket items developed on speculation for projects and situations that don't yet exist. Manufacturers are cautiously spending money on R and D for more down-to-earth items. There seems to be a motto that ''less can be more,'' if researchers focus their limited resources on developing items that are more efficient, easier to install and operate, and save money. Items reviewed on this and other pages by WORLD OIL this year are certainly reflective of such attitudes. Short on show, but long on substance, they provide practical answers to everyday, routine problems encountered by offshore operators. They range from a subsea wireline lubricator developed by BP and Schlumberger, to a new ''armoring'' system that coats platform risers and saves them from the ravages of Mother Nature. There are also an electric safety valve and assorted other goodies. In addition, engineers have completed a fascinating study, which determined why some drilling vessels really sank during blowouts. It is incorrect to assume that large-sized innovations have disappeared completely. For instance, Conoco and others have designed a single-leg, tension-leg platform for Gulf of Mexico duty, which is featured below. Keep in mind, however, the primary motivation for this design was to improve logistics, shorten the timeframe, and save money.

  19. The information seeking and procurment needs of attendees at an industrial trade show

    Directory of Open Access Journals (Sweden)

    N. C. Bresler

    2009-12-01

    Full Text Available Purpose: The purpose of this article is to describe what attracts visitors to an industrial trade show, and to profile them. This will enable the show organisers to attract the right mix of exhibitors and fulfil their dual role of satisfying the needs of both attendees and exhibitors and improve the role of exhibitions in the latter's marketing mix. Problem investigated: The research seeks to elicit the attendance objectives of participants in order to ascertain their information seeking and procurement needs. This can be used to improve the marketing communication of both the organisers and exhibitors. Research methodology: The research design is a multi method, descriptive study. A non probability, judgemental sample was drawn; 1020 interviews were conducted per Electra Mining Africa expo in 2004 and 2006. Both open ended and fixed response questions were posed and due to the similarity of responses, 300 per show were analysed. The researcher fulfilled a participant observer role to enhance the validity and reliability of the findings. Findings/implications: The prime reason for visitation was to see what is new, discover, and gather information, and attendees were not disappointed in that. The trade shows attracted an informed, niche audience. Exhibitors gained access to key decision makers with buying influence. Attendees represented all roles in the buying process and intended to buy some capital items exhibited within the following year. Business contacts were made. The attraction and contact efficiency of the exhibition's were high and may result in conversion efficiency. In terms of market and geographical coverage it is a vertical international show. Originality: This paper contributes to limited research conducted on trade shows, especially in South Africa. It is unique in that it describes the effectiveness of an industrial trade show from a demand perspective in order to improve the facilitating role of exhibition organisers. It

  20. Curcumin Shows Antiviral Properties against Norovirus.

    Science.gov (United States)

    Yang, Minji; Lee, GilJae; Si, Jiyeon; Lee, Sung-Joon; You, Hyun Ju; Ko, GwangPyo

    2016-10-20

    Phytochemicals provide environmentally friendly and relatively inexpensive natural products, which could potentially benefit public health by controlling human norovirus (HuNoV) infection. In this study, 18 different phytochemicals were evaluated for antiviral effects against norovirus using murine norovirus (MNV) as a model for norovirus biology. Among these phytochemicals, curcumin (CCM) was the most potent anti-noroviral phytochemical, followed by resveratrol (RVT). In a cell culture infection model, exposure to CCM or RVT for 3 days reduced infectivity of norovirus by 91% and 80%, respectively. To confirm the antiviral capability of CCM, we further evaluated its antiviral efficacy at various doses (0.25, 0.5, 0.75, 1, and 2 mg/mL) and durations (short-term: 10, 30, 60, and 120 min; long-term: 1, 3, 7, and 14 days). The anti-noroviral effect of CCM was verified to occur in a dose-dependent manner. Additionally, we evaluated the inhibitory effect of each phytochemical on the replication of HuNoV using a HuNoV replicon-bearing cell line (HG23). Neither CCM nor RVT had a strong inhibitory effect on HuNoV replication, which suggests that their antiviral mechanism may involve viral entry or other life cycle stages rather than the replication of viral RNA. Our results demonstrated that CCM may be a promising candidate for development as an anti-noroviral agent to prevent outbreaks of foodborne illness.

  1. Revisiting internal gravity waves analysis using GPS RO density profiles: comparison with temperature profiles and application for wave field stability study

    Directory of Open Access Journals (Sweden)

    P. Pisoft

    2018-01-01

    Full Text Available We revise selected findings regarding the utilization of Global Positioning System radio occultation (GPS RO density profiles for the analysis of internal gravity waves (IGW, introduced by Sacha et al. (2014. Using various GPS RO datasets, we show that the differences in the IGW spectra between the dry-temperature and dry-density profiles that were described in the previous study as a general issue are in fact present in one specific data version only. The differences between perturbations in the temperature and density GPS RO profiles do not have any physical origin, and there is not the information loss of IGW activity that was suggested in Sacha et al. (2014. We investigate the previously discussed question of the temperature perturbations character when utilizing GPS RO dry-temperature profiles, derived by integration of the hydrostatic balance. Using radiosonde profiles as a proxy for GPS RO, we provide strong evidence that the differences in IGW perturbations between the real and retrieved temperature profiles (which are based on the assumption of hydrostatic balance include a significant nonhydrostatic component that is present sporadically and might be either positive or negative. The detected differences in related spectra of IGW temperature perturbations are found to be mostly about ±10 %. The paper also presents a detailed study on the utilization of GPS RO density profiles for the characterization of the wave field stability. We have analyzed selected stability parameters derived from the density profiles together with a study of the vertical rotation of the wind direction. Regarding the Northern Hemisphere the results point to the western border of the Aleutian high, where potential IGW breaking is detected. These findings are also supported by an analysis of temperature and wind velocity profiles. Our results confirm advantages of the utilization of the density profiles for IGW analysis.

  2. Career Profiles of Generation Y and Their Potential Influencers

    Directory of Open Access Journals (Sweden)

    Helena Talita Dante Cordeiro

    2017-10-01

    Full Text Available This study aims to identify the predominant career profiles among Generation Y in Brazil and investigate the influence of demographic and professional characteristics in these profiles. Career profiles are defined as combinations of the presence of Boundaryless and Protean career attitudes. This study is descriptive, the sample is non-probabilistic and intentional and consists of 2,376 people. Cluster Analysis and Chi-square tests were used. The results show that people from Generation Y have a high presence of attitudes related to new careers and that these new careers are associated with high education and high income, mainly the Boundaryless career. However, there is a small portion of the sample that doesn’t present new career attitudes, indicating that this is just a movement and cannot be considered a reality for all studied subjects.

  3. Perfil del comportamiento sexual en adolescentes mexicanos de 12 a 19 años de edad: resultados de la ENSA 2000 Profile of sexual behavior in 12 to 19 year-old Mexican adolescents: results of ENSA 2000

    Directory of Open Access Journals (Sweden)

    Carlos González-Garza

    2005-06-01

    embarazos.OBJECTIVE: To describe traits associated with sexual behaviors in Mexican adolescents, their knowledge about contraception, and factors associated with pregnancy and utilization of contraceptives during first sexual intercourse. MATERIAL AND METHODS: Data from the Mexican National Health Survey 2000 (ENSA 2000 were analyzed. This study, conducted between September 1999 and March 2000, was a complex survey with a probabilistic, stratified, and cluster sampling design. The sample population included 15 241 adolescents 12 to 19 years of age. Data analysis included descriptive statistics and a chi-squared test for differences of proportions; also, logistic regression was used to obtain odds ratios. RESULTS: A total of 69.2% adolescents reported knowledge of at least one contraception method; 16.4% of subjects reported having had sexual intercourse. Males initiated sexual intercourse earlier than females and only 37% of all adolescents utilized contraceptives during their first sexual intercourse. Logistic regression analysis showed that being male, having a higher education, having knowledge of at least one contraception method, and having initiated sexual intercourse at an older age, were factors associated with utilization of a contraception method in their first sexual intercourse. A total of 55.7% of sexually active female adolescents had been pregnant. Pregnancy among adolescents was associated with low educational level, sexual activity at early age, and to have sometime lived in free union. CONCLUSIONS: The majority of sexually active adolescents did not use contraception during their first sexual intercourse, rendering them at risk of unwanted pregnancies.

  4. Correction of unevenness in recycler beam profile

    Energy Technology Data Exchange (ETDEWEB)

    Crisp, J.; Hu, M.; Ng, K.Y.; /Fermilab

    2006-05-01

    A beam confined between two rf barriers in the Fermilab Recycler Ring exhibits very uneven longitudinal profile. This leads to the consequence that the momentum-mined antiproton bunches will have an intolerable variation in bunch intensity. The observed profile unevenness is the result of a tiny amount of rf imperfection and rf beam-loading. The profile unevenness can be flattened by feeding back the uneven rf fan-back gap voltage to the low-level rf.

  5. Showing Emulsion Properties with Common Dairy Foods

    Science.gov (United States)

    Bravo-Diaz, Carlos; Gonzalez-Romero, Elisa

    1996-09-01

    Foods are mixtures of different chemical compounds, and the quality we sense (taste, texture, color, etc.) are all manifestations of its chemical properties. Some of them can be visualized with the aid of simple, safe and inexpensive experiments using dairy products that can be found in any kitchen and using almost exclusively kitchen utensils. In this paper we propose some of them related with food emulsions. Food emulsions cover an extremely wide area of daily-life applications such as milk, sauces, dressings and beverages. Experimentation with some culinary recipes to prepare them and the analyisis of the observed results is close to ideal subject for the introduction of chemical principles, allowing to discuss about the nature and composition of foods, the effects of additives, etc. At the same time it allows to get insights into the scientific reasons that underlie on the recipes (something that it is not usually found in most cookbooks). For example, when making an emulsion like mayonnaise, why the egg yolks and water are the first materials in the bowl , and the oil is added to them rather than in the other way around? How you can "rescue" separate emulsions (mayonnaise)? Which parameters affect emulsion stability? Since safety, in its broad sense, is the first requisite for any food, concerns about food exist throughout the world and the more we are aware of our everyday life, the more likely we will be to deal productively with the consequences. On the other hand, understanding what foods are and how cooking works destroys no delightful mystery of the art of cuisine, instead the mystery expands.

  6. Towards understanding tree root profiles: simulating hydrologically optimal strategies for root distribution

    Directory of Open Access Journals (Sweden)

    M. T. van Wijk

    2001-01-01

    Full Text Available In this modelling study differences in vertical root distributions measured in four contrasting forest locations in the Netherlands were investigated. Root distributions are seen as a reflection of the plant’s optimisation strategy, based on hydrological grounds. The 'optimal' root distribution is defined as the one that maximises the water uptake from the root zone over a period of ten years. The optimal root distributions of four forest locations with completely different soil physical characteristics are calculated using the soil hydrological model SWIF. Two different model configurations for root interactions were tested: the standard model configuration in which one single root profile was used (SWIF-NC, and a model configuration in which two root profiles compete for the same available water (SWIF-C. The root profiles were parameterised with genetic algorithms. The fitness of a certain root profile was defined as the amount of water uptake over a simulation period of ten years. The root profiles of SWIF-C were optimised using an evolutionary game. The results showed clear differences in optimal root distributions between the various sites and also between the two model configurations. Optimisation with SWIF-C resulted in root profiles that were easier to interpret in terms of feasible biological strategies. Preferential water uptake in wetter soil regions was an important factor for interpretation of the simulated root distributions. As the optimised root profiles still showed differences with measured profiles, this analysis is presented, not as the final solution for explaining differences in root profiles of vegetation but as a first step using an optimisation theory to increase understanding of the root profiles of trees. Keywords: forest hydrology, optimisation, roots

  7. Sensing the wind profile

    Energy Technology Data Exchange (ETDEWEB)

    Pena, A.

    2009-03-15

    This thesis consists of two parts. The first is a synopsis of the theoretical progress of the study that is based on a number of journal papers. The papers, which constitute the second part of the report, aim to analyze, measure, and model the wind prole in and beyond the surface layer by combining observations from cup anemometers with lidars. The lidar is necessary to extend the measurements on masts at the Horns Rev offshore wind farm and over at land at Hoevsoere, Denmark. Both sensing techniques show a high degree of agreement for wind speed measurements performed at either sites. The wind speed measurements are averaged for several stability conditions and compare well with the surface-layer wind profile. At Hoevsoere, it is sufficient to scale the wind speed with the surface friction velocity, whereas at Horns Rev a new scaling is added, due to the variant roughness length. This new scaling is coupled to wind prole models derived for flow over the sea and tested against the wind proles up to 160 m at Horns Rev. The models, which account for the boundary-layer height in stable conditions, show better agreement with the measurements than compared to the traditional theory. Mixing-length parameterizations for the neutral wind prole compare well with length-scale measurements up to 300 m at Hoevsoere and 950 m at Leipzig. The mixing-length-derived wind proles strongly deviate from the logarithmic wind prole, but agree better with the wind speed measurements. The length-scale measurements are compared to the length scale derived from a spectral analysis performed up to 160 m at Hoevsoere showing high agreement. Mixing-length parameterizations are corrected to account for stability and used to derive wind prole models. These compared better to wind speed measurements up to 300 m at Hoevsoere than the surface-layer wind prole. The boundary-layer height is derived in nearneutral and stable conditions based on turbulent momentum uxes only and in unstable conditions

  8. CIVIL ENGINEER PROFILE: A VIEW FROM HIS GENERIC AND SPECIFIC COMPETENCES

    Directory of Open Access Journals (Sweden)

    Daniela Brenzini

    2012-07-01

    Full Text Available This paper was aimed to determine the presence of generic and specific competences in the profile of civil engineering as a graduate of the Eastern Coast of Lake Campus of the University of Zulia; is part of a larger project. The study was carried out through a descriptive, transectional and field research. The population consisted of 38 companies. We applied a questionnaire with closed questions. The results showed the importance that generic and specific competences present in the profile of Civil Engineer; we investigated the presence of very few powers of this kind in the profile of Civil Engineers under investigation.

  9. 3D profile measurement of large-scale curvature plates using structured light source

    Science.gov (United States)

    Heo, EunChang; Kim, ByoungChang; Lee, Hyunho; Han, JongMan

    2008-08-01

    In heavy industry, especially in the shipbuilding process, 3D profile measurement of large-scale hull pieces is needed for fabrication and assembly. Currently, using many kinds of templates made of wood or plastic still do an important role as a standard ruler. We suggest an efficient method of 3D profile measurement to obtain the xyz-coordinates of curvature plates. The measurement system comprises multiple line structured laser sources and performs profile measurement by projecting structured light source on the object surface. The measurement results show that measurement accuracy is within the boundary of accuracy required in the shipbuilding process.

  10. DNA microarray profiling of a diverse collection of nosocomial methicillin-resistant staphylococcus aureus isolates assigns the majority to the correct sequence type and staphylococcal cassette chromosome mec (SCCmec) type and results in the subsequent identification and characterization of novel SCCmec-SCCM1 composite islands.

    LENUS (Irish Health Repository)

    Shore, Anna C

    2012-10-01

    One hundred seventy-five isolates representative of methicillin-resistant Staphylococcus aureus (MRSA) clones that predominated in Irish hospitals between 1971 and 2004 and that previously underwent multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing were characterized by spa typing (175 isolates) and DNA microarray profiling (107 isolates). The isolates belonged to 26 sequence type (ST)-SCCmec types and subtypes and 35 spa types. The array assigned all isolates to the correct MLST clonal complex (CC), and 94% (100\\/107) were assigned an ST, with 98% (98\\/100) correlating with MLST. The array assigned all isolates to the correct SCCmec type, but subtyping of only some SCCmec elements was possible. Additional SCCmec\\/SCC genes or DNA sequence variation not detected by SCCmec typing was detected by array profiling, including the SCC-fusidic acid resistance determinant Q6GD50\\/fusC. Novel SCCmec\\/SCC composite islands (CIs) were detected among CC8 isolates and comprised SCCmec IIA-IIE, IVE, IVF, or IVg and a ccrAB4-SCC element with 99% DNA sequence identity to SCC(M1) from ST8\\/t024-MRSA, SCCmec VIII, and SCC-CI in Staphylococcus epidermidis. The array showed that the majority of isolates harbored one or more superantigen (94%; 100\\/107) and immune evasion cluster (91%; 97\\/107) genes. Apart from fusidic acid and trimethoprim resistance, the correlation between isolate antimicrobial resistance phenotype and the presence of specific resistance genes was ≥97%. Array profiling allowed high-throughput, accurate assignment of MRSA to CCs\\/STs and SCCmec types and provided further evidence of the diversity of SCCmec\\/SCC. In most cases, array profiling can accurately predict the resistance phenotype of an isolate.

  11. Kinome profiling of Arabidopsis using arrays of kinase consensus substrates

    Directory of Open Access Journals (Sweden)

    Pieterse Corné MJ

    2007-02-01

    Full Text Available Abstract Background Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since substrates from many organisms are present we decided to test these arrays for the determination of kinase activities in the model plant species Arabidopsis thaliana. Results Kinome profiling using Arabidopsis cell extracts resulted in the labelling of many consensus peptides by kinases from the plant, indicating the usefulness of this kinome profiling tool for plants. Method development showed that fresh and frozen plant material could be used to make cell lysates containing active kinases. Dilution of the plant extract increased the signal to noise ratio and non-radioactive ATP enhances full development of spot intensities. Upon infection of Arabidopsis with an avirulent strain of the bacterial pathogen Pseudomonas syringae pv. tomato, we could detect differential kinase activities by measuring phosphorylation of consensus peptides. Conclusion We show that kinome profiling on arrays with consensus substrates can be used to monitor kinase activities in plants. In a case study we show that upon infection with avirulent P. syringae differential kinase activities can be found. The PepChip can for example be used to purify (unknown kinases that play a role in P. syringae infection. This paper shows that kinome profiling using arrays of consensus peptides is a valuable new tool to study signal-transduction in plants. It complements the available methods for genomics and proteomics research.

  12. Serum iron, ferritin, transferrin and haptoglobin concentration variations during repeated show jumping competition in horse

    Directory of Open Access Journals (Sweden)

    Anna Assenza

    2016-01-01

    Full Text Available Modifications of the iron profile in athlete horses during two international three star (*** show jumping competitions performed in two consecutive weekends were evaluated. Serum iron, ferritin, transferrin, and haptoglobin were assessed in 12 well-trained Italian Saddle horses. Blood samplings were performed before the first day of competition (R1, within 10 min from the end of each competition (J1, J2 and on the day after competition (R2. The same plan was followed during the second weekend (J3, J4 and R3. One-way repeated measures analysis of variance (ANOVA was applied on obtained data, and a significant effect of exercise (P < 0.05 on all studied indices was found. These results suggest that serum iron, transferrin, ferritin and haptoglobin are responsive to intense exercise and could be considered important indicators that may give important information about the horse’s performance.

  13. Motivational Profiles of Adult Learners

    Science.gov (United States)

    Rothes, Ana; Lemos, Marina S.; Gonçalves, Teresa

    2017-01-01

    This study investigated profiles of autonomous and controlled motivation and their effects in a sample of 188 adult learners from two Portuguese urban areas. Using a person-centered approach, results of cluster analysis and multivariate analysis of covariance revealed four motivational groups with different effects in self-efficacy, engagement,…

  14. Secondary maxima in ozone profiles

    Directory of Open Access Journals (Sweden)

    R. Lemoine

    2004-01-01

    Full Text Available Ozone profiles from balloon soundings as well as SAGEII ozone profiles were used to detect anomalous large ozone concentrations of ozone in the lower stratosphere. These secondary ozone maxima are found to be the result of differential advection of ozone-poor and ozone-rich air associated with Rossby wave breaking events. The frequency and intensity of secondary ozone maxima and their geographical distribution is presented. The occurrence and amplitude of ozone secondary maxima is connected to ozone variability and trend at Uccle and account for a large part of the total ozone and lower stratospheric ozone variability.

  15. Transposon identification using profile HMMs

    Science.gov (United States)

    2010-01-01

    Background Transposons are "jumping genes" that account for large quantities of repetitive content in genomes. They are known to affect transcriptional regulation in several different ways, and are implicated in many human diseases. Transposons are related to microRNAs and viruses, and many genes, pseudogenes, and gene promoters are derived from transposons or have origins in transposon-induced duplication. Modeling transposon-derived genomic content is difficult because they are poorly conserved. Profile hidden Markov models (profile HMMs), widely used for protein sequence family modeling, are rarely used for modeling DNA sequence families. The algorithm commonly used to estimate the parameters of profile HMMs, Baum-Welch, is prone to prematurely converge to local optima. The DNA domain is especially problematic for the Baum-Welch algorithm, since it has only four letters as opposed to the twenty residues of the amino acid alphabet. Results We demonstrate with a simulation study and with an application to modeling the MIR family of transposons that two recently introduced methods, Conditional Baum-Welch and Dynamic Model Surgery, achieve better estimates of the parameters of profile HMMs across a range of conditions. Conclusions We argue that these new algorithms expand the range of potential applications of profile HMMs to many important DNA sequence family modeling problems, including that of searching for and modeling the virus-like transposons that are found in all known genomes. PMID:20158867

  16. TOPOLOGICAL AGGREGATION, THE TWIN PARADOX AND THE NO SHOW PARADOX

    OpenAIRE

    Chèze, Guillaume

    2016-01-01

    Consider the framework of topological aggregation introduced by Chichilnisky (1980). We prove that in this framework the Twin Paradox and the No Show Paradox cannot be avoided. Anonymity and unanimity are not needed to obtain these results.

  17. Characterisation and concentration profile of aluminium during ...

    African Journals Online (AJOL)

    Characterisation and concentration profile of aluminium during drinking-water treatment. ... at a surface water treatment plant (Buffalo Pound Water Treatment Plant ... Seasonal evaluations of Al at BPWTP showed that raw lake water total ...

  18. Intercultural Profiles and Adaptation Among Immigrant and Autochthonous Adolescents.

    Science.gov (United States)

    Inguglia, Cristiano; Musso, Pasquale

    2015-02-01

    Few studies examine relationships between intercultural strategies and adaptation among adolescents using a person-oriented approach. Framed from an intercultural psychology perspective, this study used such an approach in order to examine the influence of intercultural profiles, patterns of relationships among variables related to intercultural strategies, on the adaptation of adolescents of both non-dominant and dominant groups. Two hundred and fifty-six adolescents living in Italy and aged from 14 to 18 participated to the study: 127 immigrants from Tunisia (males = 49.61%) and 129 autochthonous (males = 44.19%). Data were collected through self-report questionnaires. Using cluster analytic methods to identify profiles, the results showed that immigrant adolescents were divided in two acculturation profiles, ethnic and integrated-national, with adolescents belonging to the latter showing higher self-esteem, life satisfaction and sociocultural competence than the former. Also among autochthonous adolescents two acculturation expectation profiles were identified, not-multicultural and multicultural, with adolescents belonging to the latter showing higher self-esteem and life satisfaction than the former. Findings highlight the importance of using multiple indicators in order to gain a more comprehensive understanding of the acculturation process as well as suggesting implications for the social policies in this field.

  19. Intercultural Profiles and Adaptation Among Immigrant and Autochthonous Adolescents

    Directory of Open Access Journals (Sweden)

    Cristiano Inguglia

    2015-02-01

    Full Text Available Few studies examine relationships between intercultural strategies and adaptation among adolescents using a person-oriented approach. Framed from an intercultural psychology perspective, this study used such an approach in order to examine the influence of intercultural profiles, patterns of relationships among variables related to intercultural strategies, on the adaptation of adolescents of both non-dominant and dominant groups. Two hundred and fifty-six adolescents living in Italy and aged from 14 to 18 participated to the study: 127 immigrants from Tunisia (males = 49.61% and 129 autochthonous (males = 44.19%. Data were collected through self-report questionnaires. Using cluster analytic methods to identify profiles, the results showed that immigrant adolescents were divided in two acculturation profiles, ethnic and integrated-national, with adolescents belonging to the latter showing higher self-esteem, life satisfaction and sociocultural competence than the former. Also among autochthonous adolescents two acculturation expectation profiles were identified, not-multicultural and multicultural, with adolescents belonging to the latter showing higher self-esteem and life satisfaction than the former. Findings highlight the importance of using multiple indicators in order to gain a more comprehensive understanding of the acculturation process as well as suggesting implications for the social policies in this field.

  20. Modeling of heat generations for different tool profiles in friction stir welding: study of tool geometry and contact conditions

    Directory of Open Access Journals (Sweden)

    Akindoye Waheed

    2017-04-01

    Full Text Available In this work, improved heat generation models are developed for straight and tapered shoulder geometries with different tool pin profiles in friction stir welding. The models are developed considering the welding process as a combination of the pure sliding and the pure sticking conditions. From the results, the amount of heat generation is directly proportional to the number of edges in the pin profiles in such a way that the heat generated in the profiles increases from the triangular pin profile to hexagonal pin profile. Also, increase in the tool rotational speed under constant weld speed increases the heat input while increase in the weld speed under constant tool rotational speed decreases the heat input and the rate of heat generation at the shoulder in a flat shoulder tool is more than that of conical/tapered shoulder tool. The predicted results show good agreements with the experimental results in literature.

  1. Optimizing UV laser focus profiles for improved MALDI performance.

    Science.gov (United States)

    Holle, Armin; Haase, Andreas; Kayser, Markus; Höhndorf, Jens

    2006-06-01

    Matrix assisted laser desorption/ionization (MALDI) applications, such as proteomics, genomics, clinical profiling and MALDI imaging, have created a growing demand for faster instrumentation. Since the commonly used nitrogen lasers have throughput and life span limitations, diode-pumped solid-state lasers are an alternative. Unfortunately this type of laser shows clear performance limitations in MALDI in terms of sensitivity, resolution and ease of use, for applications such as thin-layer sample preparations, acceptance of various matrices (e.g. DHB for glycopeptides) and MALDI imaging. While it is obvious that the MALDI process has some dependence on the characteristics of the laser used, it is unclear which features are the most critical in determining laser performance for MALDI. In this paper we show, for the first time, that a spatially structured laser beam profile in lieu of a Gaussian profile is of striking importance. This result enabled us to design diode-pumped Nd : YAG lasers that on various critical applications perform as well for MALDI as the nitrogen lasers and in some respects even better. The modulation of the beam profile appears to be a new parameter for optimizing the MALDI process. In addition, the results trigger new questions directing us to a better understanding of the MALDI process. Copyright (c) 2006 John Wiley & Sons, Ltd.

  2. Somatic symptom profiles in the general population

    DEFF Research Database (Denmark)

    Eliasen, Marie; Jørgensen, Torben; Schröder, Andreas

    2017-01-01

    PURPOSE: The aim of this study was to identify and describe somatic symptom profiles in the general adult population in order to enable further epidemiological research within multiple somatic symptoms. METHODS: Information on 19 self-reported common somatic symptoms was achieved from a population....... The profiles were further described by their association with age, sex, chronic disease, and self-perceived health. RESULTS: We identified 10 different somatic symptom profiles defined by number, type, and site of the symptoms. The majority of the population (74.0%) had a profile characterized...... population-based studies with specific focus on symptom burden....

  3. Aerodynamics profile not in stationary flow

    Directory of Open Access Journals (Sweden)

    А.А. Загорулько

    2006-02-01

    Full Text Available  Consider the question about influence of unsteady flight on the size of drag and lift coefficients of theaerodynamic profile. Distinctive features of this investigation are obtaining data about aerodynamic drag chancing in process unsteady on high angle at attack and oscillation profile in subsonic and transonic flight. Given analysis of oscillation profile show, that dynamic loops accompany change of lift and dray force. The researches show that it is necessary to clarity the mathematic model of the airplane flight dynamics by introducing numbers, with take into account unsteady effects.

  4. Temperamental profiles and language development: a replication and an extension.

    Science.gov (United States)

    Garello, Valentina; Viterbori, Paola; Usai, M Carmen

    2012-02-01

    Individual differences in child temperament are associated with individual differences in language development. The present study examined the relationship between temperament and language ability in 109 twenty-four- to 30-month-old children. Parents and day-care teachers completed two questionnaires: the Primo Vocabolario del Bambino (Caselli & Casadio, 1995) and the Questionari Italiani del Temperamento (Axia, 2002). Researchers administered the First Language Test (Axia, 1993) to assess productive and receptive language in each child. Replicating previous research (Usai, Garello, & Viterbori, 2009), day-care teachers identified three temperamental profiles: most of the children fit into the first profile, typical of the Italian population; another profile was made up of easily distractible and not very persistent children, with a poor capacity to modulate motor activity; and the third profile of children were inhibited in new situations. A relationship was found between temperament assessed by day-care teachers and different levels of linguistic competence. In particular, the groups of "inattentive" and "inhibited" children showed poorer lexical and morphological abilities and a more immature vocabulary, characterised by the presence of more primitive components of the lexical repertory compared to the group of "typical" children. Unlike the results from day-care teachers, temperament questionnaires completed by parents revealed a 4-cluster-solution. Also, for parents, the "typical" profile is characterised by the largest vocabulary (productive and receptive) and the most mature semantic production. Copyright © 2011 Elsevier Inc. All rights reserved.

  5. Diffusion pore imaging with generalized temporal gradient profiles.

    Science.gov (United States)

    Laun, Frederik B; Kuder, Tristan A

    2013-09-01

    In porous material research, one main interest of nuclear magnetic resonance diffusion (NMR) experiments is the determination of the shape of pores. While it has been a longstanding question if this is in principle achievable, it has been shown recently that it is indeed possible to perform NMR-based diffusion pore imaging. In this work we present a generalization of these previous results. We show that the specific temporal gradient profiles that were used so far are not unique as more general temporal diffusion gradient profiles may be used. These temporal gradient profiles may consist of any number of "short" gradient pulses, which fulfil the short-gradient approximation. Additionally, "long" gradient pulses of small amplitude may be present, which can be used to fulfil the rephasing condition for the complete profile. Some exceptions exist. For example, classical q-space gradients consisting of two short gradient pulses of opposite sign cannot be used as the phase information is lost due to the temporal antisymmetry of this profile. Copyright © 2013 Elsevier Inc. All rights reserved.

  6. Phenotypic Variation Profile of Marsilea crenata Presl. Cultivated in Water and in the Soil

    Directory of Open Access Journals (Sweden)

    Mangestuti Agil

    2017-01-01

    Full Text Available The aim of this research was to investigate possible phenotypic variation profile of Marsilea crenata Presl. cultivated in water and in the soil, to find alternative cultivation techniques to fulfill the increasing demand for pollutant-free plants. Phenotypic profile was investigated through macroscopic and microscopic examinations of all plant parts, and phytochemical screening on the ethanol extract of the leaves using thin layer chromatography technique was conducted to detect the terpenoid constituents. Results showed a variation of phenotypic profiles in the macroscopic examination caused by different cultivation methods. Phytochemical screening showed spots of terpenoid compounds with different color intensity. No differences were found in the microscopic examination of the leaves, petioles, and stems. Since major profile changes did not occur, M. crenata is recommended to be cultivated in water as its original habitat by providing a better caring and quality of water. Due to its phytoremediation property, it is necessary to grow M. crenata in pollutant-free water.

  7. Accurate, fully-automated NMR spectral profiling for metabolomics.

    Science.gov (United States)

    Ravanbakhsh, Siamak; Liu, Philip; Bjorndahl, Trent C; Bjordahl, Trent C; Mandal, Rupasri; Grant, Jason R; Wilson, Michael; Eisner, Roman; Sinelnikov, Igor; Hu, Xiaoyu; Luchinat, Claudio; Greiner, Russell; Wishart, David S

    2015-01-01

    Many diseases cause significant changes to the concentrations of small molecules (a.k.a. metabolites) that appear in a person's biofluids, which means such diseases can often be readily detected from a person's "metabolic profile"-i.e., the list of concentrations of those metabolites. This information can be extracted from a biofluids Nuclear Magnetic Resonance (NMR) spectrum. However, due to its complexity, NMR spectral profiling has remained manual, resulting in slow, expensive and error-prone procedures that have hindered clinical and industrial adoption of metabolomics via NMR. This paper presents a system, BAYESIL, which can quickly, accurately, and autonomously produce a person's metabolic profile. Given a 1D 1H NMR spectrum of a complex biofluid (specifically serum or cerebrospinal fluid), BAYESIL can automatically determine the metabolic profile. This requires first performing several spectral processing steps, then matching the resulting spectrum against a reference compound library, which contains the "signatures" of each relevant metabolite. BAYESIL views spectral matching as an inference problem within a probabilistic graphical model that rapidly approximates the most probable metabolic profile. Our extensive studies on a diverse set of complex mixtures including real biological samples (serum and CSF), defined mixtures and realistic computer generated spectra; involving > 50 compounds, show that BAYESIL can autonomously find the concentration of NMR-detectable metabolites accurately (~ 90% correct identification and ~ 10% quantification error), in less than 5 minutes on a single CPU. These results demonstrate that BAYESIL is the first fully-automatic publicly-accessible system that provides quantitative NMR spectral profiling effectively-with an accuracy on these biofluids that meets or exceeds the performance of trained experts. We anticipate this tool will usher in high-throughput metabolomics and enable a wealth of new applications of NMR in

  8. Accurate, fully-automated NMR spectral profiling for metabolomics.

    Directory of Open Access Journals (Sweden)

    Siamak Ravanbakhsh

    Full Text Available Many diseases cause significant changes to the concentrations of small molecules (a.k.a. metabolites that appear in a person's biofluids, which means such diseases can often be readily detected from a person's "metabolic profile"-i.e., the list of concentrations of those metabolites. This information can be extracted from a biofluids Nuclear Magnetic Resonance (NMR spectrum. However, due to its complexity, NMR spectral profiling has remained manual, resulting in slow, expensive and error-prone procedures that have hindered clinical and industrial adoption of metabolomics via NMR. This paper presents a system, BAYESIL, which can quickly, accurately, and autonomously produce a person's metabolic profile. Given a 1D 1H NMR spectrum of a complex biofluid (specifically serum or cerebrospinal fluid, BAYESIL can automatically determine the metabolic profile. This requires first performing several spectral processing steps, then matching the resulting spectrum against a reference compound library, which contains the "signatures" of each relevant metabolite. BAYESIL views spectral matching as an inference problem within a probabilistic graphical model that rapidly approximates the most probable metabolic profile. Our extensive studies on a diverse set of complex mixtures including real biological samples (serum and CSF, defined mixtures and realistic computer generated spectra; involving > 50 compounds, show that BAYESIL can autonomously find the concentration of NMR-detectable metabolites accurately (~ 90% correct identification and ~ 10% quantification error, in less than 5 minutes on a single CPU. These results demonstrate that BAYESIL is the first fully-automatic publicly-accessible system that provides quantitative NMR spectral profiling effectively-with an accuracy on these biofluids that meets or exceeds the performance of trained experts. We anticipate this tool will usher in high-throughput metabolomics and enable a wealth of new applications of

  9. High Resolution Optical Spectroscopy of the Classical Nova V5668 Sgr Showing the Presence of Lithium

    Science.gov (United States)

    Wagner, R. Mark; Woodward, Charles E.; Starrfield, Sumner; Ilyin, Ilya; Strassmeier, Klaus

    2018-01-01

    The classical nova (CN) V5668 Sgr was discovered on 2015 March 15.634 and initial optical spectra implied it was an Fe II-class CN. We obtained high resolution optical spectroscopy on 30 nights between 2015 April 3 and 2016 June 5 with the 2 x 8.4 m Large Binocular Telescope (LBT) and the 1.8 m Vatican Advanced Technology Telescope (VATT) using the Potsdam Echelle Polarimetric Spectroscopic Instrument (PEPSI). The spectra cover all or part of the 3830-9065 Å spectral region at a spectral resolution of up to 270,000 (1 km/s); the highest resolution currently available on any 8-10 m class telescope. The early spectra are dominated by emission lines of the Balmer and Paschen series of hydrogen, Fe II, Ca II, and Na I with P Cyg-type line profiles as well as emission lines of [O I]. Numerous interstellar lines and bands are readily apparent at high spectral resolution. The permitted line profiles show complex and dramatic variations in the multi-component P Cyg-type line profiles with time. We detect a weak blue-shifted absorption line at a velocity consistent with Li I 6708 Å when compared with the line profiles of Hβ, Fe II 5169 Å, and Na I D. This line is present in spectra obtained on 7 of 8 consecutive nights up to day 21 of the outburst; but absent on day 42 when it is evident that the ionization of the ejecta has significantly increased. The equivalent width of the line converted to a column density, and the resulting mass fraction, imply a significant enrichment of 7Li in the ejecta. 7Li is produced by the decay of unstable 7Be created during the thermonuclear runaway. The discovery of the resonance lines of 7Be II in the optical spectra of the recent CNe V339 Del, V2944 Oph, and V5668 Sgr by Tajitsu et al. (2016) and its subsequent decay to 7Li (half life of 53 days) suggests a significant enrichment of 7Li in the Galaxy from CNe is possible. Our observations of the Li I 6708 Å line in the early optical spectra of V5668 Sgr mark the second direct

  10. Flood-profile analysis, Big Darby Creek at State Route 762, Orient, Ohio

    Science.gov (United States)

    Bartlett, W.P.; Sherwood, J.M.

    1984-01-01

    The U.S. Geological Survey, in cooperation with the Ohio Department of Transportation, made a hydraulic analysis of the 25- and 100-year floods on Big Darby Creek where State Route 762 crosses the stream at Orient, Ohio. Two proposed bridge plans were analyzed to determine the effects on flood profiles subsequent to the placement of a 376-foot-long, four-span deck bridge across the stream 90 feet upstream of the existing State Route 762 bridge. In plan 1, the brigde is set at a 25-degrees skew to the river and at sufficient elevation to pass the 100-year flood below it. In plan 2, the skew is 28 degrees and elevations are 3 feet lower than in plan 1, which result in partial submergence of the bridge during the 100-year flood. This analysis shows that the 25-year flood profiles upstream of the new bridge would increase by 0.4 foot if plan 1 is adopted and by 0.3 foot if plan 2 is adopted. Both profiles converge with the present-condition profiles 5,750 feet upstream. The profiles for the 100-year flood would increase by 0.6 foot plan 1 and 1.1 feet for plan 2. This additional backwater affects profiles is 0.1 foot higher than for present conditions, and the plan 2 profile is 0.2 foot higher than profiles for present conditions.

  11. Flupyradifurone: a brief profile of a new butenolide insecticide

    OpenAIRE

    Nauen, Ralf; Jeschke, Peter; Velten, Robert; Beck, Michael E; Ebbinghaus-Kintscher, Ulrich; Thielert, Wolfgang; W?lfel, Katharina; Haas, Matthias; Kunz, Klaus; Raupach, Georg

    2014-01-01

    BACKGROUND The development and commercialisation of new chemical classes of insecticides for efficient crop protection measures against destructive invertebrate pests is of utmost importance to overcome resistance issues and to secure sustainable crop yields. Flupyradifurone introduced here is the first representative of the novel butenolide class of insecticides active against various sucking pests and showing an excellent safety profile. RESULTS The discovery of flupyradifurone was inspired...

  12. Psychometric characteristics of The Self-Perception Profile for Children

    Directory of Open Access Journals (Sweden)

    Petra Dolenc

    2007-06-01

    Full Text Available The purpose of the study was to examine the psychometric characteristics of the Slovene version of the Self-Perception Profile for Children (Harter, 1985. Results on a sample of 480 students showed that the Slovene version of the questionnaire had construct validity and reliability comparable of those found in the original version. Although the scale represents a useful instrument to ascertain self-concept in school settings, it requires further examination and some improvements of its metric characteristics.

  13. Expression of P190 and P210 BCR/ABL1 in normal human CD34(+) cells induces similar gene expression profiles and results in a STAT5-dependent expansion of the erythroid lineage

    DEFF Research Database (Denmark)

    Järås, Marcus; Johnels, Petra; Agerstam, Helena

    2009-01-01

    OBJECTIVE: The P190 and P210 BCR/ABL1 fusion genes are mainly associated with different types of hematologic malignancies, but it is presently unclear whether they are functionally different following expression in primitive human hematopoietic cells. MATERIALS AND METHODS: We investigated...... and systematically compared the effects of retroviral P190 BCR/ABL1 and P210 BCR/ABL1 expression on cell proliferation, differentiation, and global gene expression in human CD34(+) cells from cord blood. RESULTS: Expression of either P190 BCR/ABL1 or P210 BCR/ABL1 resulted in expansion of erythroid cells...... and stimulated erythropoietin-independent burst-forming unit-erythroid colony formation. By using a lentiviral anti-signal transducer and activator of transcription 5 (STAT5) short-hairpin RNA, we found that both P190 BCR/ABL1- and P210 BCR/ABL1-induced erythroid cell expansion were STAT5-dependent. Under...

  14. Nadir Ozone Profile Retrieval from SCIAMACHY: application to the Antarctic Ozone Hole

    Science.gov (United States)

    Shah, Sweta; Piet, Stammes; Tuinder, Olaf N. E.; de Laat, Jos

    2017-04-01

    We present new nadir ozone profile retrievals using SCIAMACHY UV reflectance spectra for the mission period of the Envisat satellite. We have used the most recent Level-1 data version (v8 with degradation correction included) in the UV range (265-330 nm) and have used the OPERA optimal estimation algorithm (van Peet et al., AMT, 2014) developed in KNMI. We first show the comparison of the retrieved satellite profiles to co-located ozone sonde profiles in order to evaluate the accuracy of the retrieved ozone profile dataset. Based on these results, we have further processed the SCIAMCHY nadir dataset, specifically all the southern hemisphere pixels south of 45 degrees latitude for the months of August-November for the complete years 2003-2011. We show the monthly mean profiles, time-series of daily averages and minima of the retrieved stratospheric columns, and finally the ozone profile trend over the years 2003-2011. We also show the comparison of our results with the literature and hence the consistency of this new SCIAMACHY dataset.

  15. Parent Prevention Communication Profiles and Adolescent Substance Use: A Latent Profile Analysis and Growth Curve Model

    Science.gov (United States)

    Choi, Hye Jeong; Miller-Day, Michelle; Shin, YoungJu; Hecht, Michael L.; Pettigrew, Jonathan; Krieger, Janice L.; Lee, JeongKyu; Graham, John W.

    2017-01-01

    This current study identifies distinct parent prevention communication profiles and examines whether youth with different parental communication profiles have varying substance use trajectories over time. Eleven schools in two rural school districts in the Midwestern United States were selected, and 784 students were surveyed at three time points from the beginning of 7th grade to the end of 8th grade. A series of latent profile analyses were performed to identify discrete profiles/subgroups of substance-specific prevention communication (SSPC). The results revealed a 4-profile model of SSPC: Active-Open, Passive-Open, Active-Silent, and Passive-Silent. A growth curve model revealed different rates of lifetime substance use depending on the youth’s SSPC profile. These findings have implications for parenting interventions and tailoring messages for parents to fit specific SSPC profiles. PMID:29056872

  16. Individual Profiling Using Text Analysis

    Science.gov (United States)

    2016-04-15

    likelihood that it belongs to the input text , although early experiments showed that this added no benefit. Parts–of– speech In early experiments all...tweets were POS tagged as part of the pre– processing step using a Twitter specific part–of– speech tagger [8]. Various studies have identified POS tags as...AFRL-AFOSR-UK-TR-2016-0011 Individual Profiling using Text Analysis 140333 Mark Stevenson UNIVERSITY OF SHEFFIELD, DEPARTMENT OF PSYCHOLOGY Final

  17. Nissan at 33rd Tokyo Motor Show; Dai 33 kai Tokyo Motor show

    Energy Technology Data Exchange (ETDEWEB)

    Sekiguchi, M. [Nissan Motor Co. Ltd., Tokyo (Japan)

    2000-01-01

    The Tokyo Motor Show is one of the leading exhibition opportunities for automobile companies world wide in terms of both scale and features, including the number of visitors as well as the devotion of each participant. For many years, we have worked hard to excell at each session of this show, (through the display of concept cars and Nissan Motor's technological developments), the show has played an increasing role in enhancing Nissan's brand image. At the 33rd Tokyo Motor Show in October 1999 was held at a time when Nissan had drawn the attention of the market because of its tic-up with Renault. Nissan demonstrated a revolutionary change through the introduction of concept cars, environmental technology, safety technology, new models, and so on. (author)

  18. Solid-phase microextraction-based cuticular hydrocarbon profiling for intraspecific delimitation in Acyrthosiphon pisum

    Science.gov (United States)

    Chen, Nan; Bai, Yu; Fan, Yong-Liang

    2017-01-01

    Insect cuticular hydrocarbons (CHCs) play critical roles in reducing water loss and chemical communication. Species-specific CHC profiles have been used increasingly as an excellent character for species classification. However, considerably less is known about their potential for population delimitation within species. The aims of this study were to develop a solid-phase microextraction (SPME)-based CHC collection method and to investigate whether CHC profiles could serve as potential chemotaxonomic tools for intraspecific delimitation in Acyrthosiphon pisum. Optimization of fibers for SPME sampling revealed that 7 μm polydimethylsiloxane (PDMS) demonstrated the most efficient adsorption of CHCs among five different tested fibers. SPME sampling showed good reproducibility with repeated collections of CHCs from a single aphid. Validation of SPME was performed by comparing CHC profiles with those from conventional hexane extractions. The two methods showed no qualitative differences in CHCs, although SPME appeared to extract relatively fewer short-chained CHCs. While CHC profiles of a given population differed among developmental stages, wing dimorphism types, and host plants, wingless adult aphids showed very low variance in relative proportions of individual CHC components. Reproducibility of CHC profiles was explored further to classify wingless adult morphs of A. pisum from five different geographic regions that showed no variation in mitochondrial COI gene sequences. Our results demonstrate that CHC profiles are useful in intraspecific delimitation in the field of insect chemotaxonomy. PMID:28859151

  19. Lipid Profiles of Persons With Paraplegia and Tetraplegia: Sex Differences

    Science.gov (United States)

    Schmid, Andreas; Knöebber, Judith; Vogt, Stefan; König, Daniel; Deibert, Peter; Bültermann, Dirk; Heinrich, Lothar; Baumstark, Manfred W; Berg, Aloys; Storch, Max-Jürgen

    2008-01-01

    Background/Objective: To examine the lipoprotein profiles of men and women with paraplegia and tetraplegia. Impairment of the sympathetic nervous system (dependent on the level of injury) and the extent of physical capacity and activity were correlated with the lipid profile in men with spinal cord injury (SCI). Sex-related differences of the lipoprotein profiles could be found in nondisabled and premenopausal women with SCI mainly because of the different effects of sexual hormones. Methods: Lipoprotein profiles of 112 participants with SCI (32 premenopausal women, 80 men) were analyzed and correlated to sex, lesion level, and physical performance capacity. Results: Women with tetraplegia or paraplegia showed significantly higher levels of high-density lipoprotein and lower ratios of total cholesterol to high-density lipoprotein-cholesterol compared with men with corresponding lesion levels, without a difference in peak oxygen consumption. Concentrations of very-low-density lipoproteins were lower in women with paraplegia than in men with paraplegia; no differences were found in total cholesterol, low-density lipoprotein-cholesterol, and triglycerides. Sex-independent elevations in total cholesterol and low-density lipoprotein-cholesterol were associated with paraplegia, and sex-independent elevations in triglyceride levels were associated with tetraplegia. Conclusions: Persons with SCI showed sex-related differences in their lipoprotein profiles. Independent of physical fitness, the lipoprotein profile of premenopausal women with SCI did not exhibit the adverse lipoprotein characteristics observed in men with SCI, probably because of the influence of sexual hormones independent of lesion level. PMID:18795478

  20. Patient Profile in Dermatology

    Directory of Open Access Journals (Sweden)

    Esra Adışen

    2008-10-01

    Full Text Available Background and Design: Skin diseases are among the most prevalent problems in medical practice. Dermatologists have become active, not only in treatment of skin diseases, but also for the cosmetic and surgical procedures. The purpose of this study was to evaluate the profiles of patients visiting outpatient dermatology clinic of an university hospital and a private dermatologist’s office located in Ankara. Material and Method: The study comprised 5952 patients in each group. Age, gender, diagnosis, and the places they live, were all recorded. Results: There were 3778 women and 2174 men with a mean age of 37.8±15.9 years (6 months-81 years in private visit group, and 3570 women and 2382 men with a mean age of 37.9±18.6 years (1-100 years in hospital visit group. The most common cause for visiting dermatologist’s office was acne. It was fungal diseases for hospital visits. Women with fungal disease, alopecia, nail disorders, urticaria, psychocutaneous dermatoses, bacterial infections, and men with fungal disease, acne, alopecia, urticaria, nail disorders, preferred hospitals over private offices. Both women and men with benign and malignant tumors, nevi, pigmentation disorders, preferred private office over hospital. Men with syphilis, genital herpes, anogenital verruca preferred mainly private offices. Visits to dermatologist’s office for cosmetic procedures constituted only 4% of overall visits.Conclusion: Our finding underscores the fact that medical or clinical dermatology continues to be the focus of most dermatology practices. Apart from cosmetic procedures, the main difference between patient profiles visiting private offices and hospitals is observed in sexually transmitted diseases. (Turkderm 2008; 42: 82-6

  1. Disorder-specific cognitive profiles in major depressive disorder and generalized anxiety disorder.

    Science.gov (United States)

    Hendriks, Sanne M; Licht, Carmilla M M; Spijker, Jan; Beekman, Aartjan T F; Hardeveld, Florian; de Graaf, Ron; Penninx, Brenda W J H

    2014-04-01

    This investigation examines differences in cognitive profiles in subjects with major depressive disorder (MDD) and generalized anxiety disorder (GAD). Data were used from subjects with current MDD (n = 655), GAD (n = 107) and comorbid MDD/GAD (n = 266) diagnosis from the Netherlands Study of Depression and Anxiety (NESDA). The Composite Interview Diagnostic Instrument was used to diagnose MDD and GAD. Cognitive profiles were measured using the Leiden Index of Depression Sensitivity, the Anxiety Sensitivity Index, and the Penn State Worry Questionnaire. Results showed that differences in cognitive profiles between single MDD and single GAD subjects were present: scores on hopelessness/suicidality and rumination were significantly higher in MDD than GAD, whereas anxiety sensitivity for physical concerns and pathological worry were higher in GAD than MDD. The cognitive profile of comorbid MDD/GAD showed more extreme depression cognitions compared to single disorders, and a similar anxiety profile compared to single GAD subjects. Despite the commonalities in cognitive profiles in MDD and GAD, there are differences suggesting that MDD and GAD have disorder-specific cognitive profiles. Findings of this investigation give support for models like the cognitive content-specificity model and the tripartite model and could provide useful handles for treatment focus.

  2. Scaling behavior of the Compton profile of alkali metals

    Science.gov (United States)

    Sekania, Michael; Appelt, Wilhelm H.; Benea, Diana; Ebert, Hubert; Vollhardt, Dieter; Chioncel, Liviu

    2018-01-01

    The contribution of the valence electrons to the Compton profiles of the crystalline alkali metals is calculated using density functional theory. We show that the Compton profiles can be modeled by a q-Gaussian distribution, which is characterized by an anisotropic, element dependent parameter q. Thereby we derive an unexpected scaling behavior of the Compton profiles of all alkali metals.

  3. Corn oil improves the plasma lipoprotein lipid profile compared with extra-virgin olive oil consumption in men and women with elevated cholesterol: results from a randomized controlled feeding trial.

    Science.gov (United States)

    Maki, Kevin C; Lawless, Andrea L; Kelley, Kathleen M; Kaden, Valerie N; Geiger, Constance J; Dicklin, Mary R

    2015-01-01

    Restricted intakes of saturated and trans-fatty acids is emphasized in heart-healthy diets, and replacement with poly- and monounsaturated fatty acids is encouraged. To compare the effects of polyunsaturated fatty acid-rich corn oil (CO) and monounsaturated fatty acid-rich extra-virgin olive oil (EVOO) on plasma lipids in men and women (N = 54) with fasting low-density lipoprotein cholesterol (LDL-C) ≥130 mg/dL and yogurt) as part of a weight-maintenance diet (∼35% fat, cholesterol). Subjects ate breakfast at the clinic every weekday throughout the study. Lunches, dinners, and snacks (and breakfasts on weekends) were provided for consumption away from the clinic. Baseline mean (standard error) lipids in mg/dL were: LDL-C 153.3 (3.5), total cholesterol (total-C) 225.7 (3.9), non-high-density lipoprotein (non-HDL)-C 178.3 (3.7), HDL-C 47.4 (1.7), total-C/HDL-C 5.0 (0.2), and TG 124.8 (7.2). CO resulted in significantly larger least-squares mean % changes (all P cholesterol diet resulted in more favorable changes in LDL-C and other atherogenic lipids vs EVOO. Copyright © 2015 National Lipid Association. Published by Elsevier Inc. All rights reserved.

  4. INFLAMMATORY BOWEL DISEASE: OUTPATIENT TREATMENT PROFILE

    Directory of Open Access Journals (Sweden)

    Rachael Miranda dos SANTOS

    2017-03-01

    Full Text Available ABSTRACT BACKGROUND Crohn’s disease and ulcerative colitis are the two major forms of inflammatory bowel disease. The incidence and prevalence of both conditions have increased and are progressively increasing. These diseases are frequently recurrent and clinically highly severe. In Brazil, the lack of epidemiological data related to such diseases has left these patients in a vulnerable state and contributed to increased morbidity. OBJECTIVE To describe the profiles of patients with inflammatory bowel disease treated in an outpatient service in Brazil. METHODS This descriptive, exploratory, and retrospective documentary study with a quantitative approach was performed in an outpatient treatment service for inflammatory bowel disease, at a university polyclinic located in Rio de Janeiro, Brazil, from May to July 2016. The study included 556 patients and was approved by the research ethics committee of the institution (CAAE no. 55179316.6.0000.5259/2016. RESULTS The data showed a high prevalence of inflammatory bowel disease in white female patients. Crohn’s disease was diagnosed in more patients than was ulcerative colitis; the ileocolon was the most commonly affected location in patients with Crohn’s disease. The stenotic phenotype was prevalent in patients with Crohn’s disease. CONCLUSION The prevalence of the stenotic phenotype in Crohn’s disease in relation to others demonstrates the need for further investigations in this field of study in Brazil. In conclusion, the data showed that the epidemiologic profile of the study population is similar to that published in the national and international literature.

  5. Monocytes of patients with familial hypercholesterolemia show alterations in cholesterol metabolism

    Directory of Open Access Journals (Sweden)

    Soufi Muhidien

    2008-11-01

    Full Text Available Abstract Background Elevated plasma cholesterol promotes the formation of atherosclerotic lesions in which monocyte-derived lipid-laden macrophages are frequently found. To analyze, if circulating monocytes already show increased lipid content and differences in lipoprotein metabolism, we compared monocytes from patients with Familial Hypercholesterolemia (FH with those from healthy individuals. Methods Cholesterol and oxidized cholesterol metabolite serum levels of FH and of healthy, gender/age matched control subjects were measured by combined gas chromatography – mass spectroscopy. Monocytes from patients with FH and from healthy subjects were isolated by antibody-assisted density centrifugation. Gene expression profiles of isolated monocytes were measured using Affymetrix HG-U 133 Plus 2.0 microarrays. We compared monocyte gene expression profiles from FH patients with healthy controls using a Welch T-test with correction for multiple testing (p Results Using microarray analysis we found in FH patients a significant up-regulation of 1,617 genes and a down-regulation of 701 genes compared to monocytes from healthy individuals. These include genes of proteins that are involved in the uptake, biosynthesis, disposition, and cellular efflux of cholesterol. In addition, plasma from FH patients contains elevated amounts of sterols and oxysterols. An increased uptake of oxidized as well as of native LDL by FH monocytes combined with a down-regulation of NPC1 and ABCA1 explains the lipid accumulation observed in these cells. Conclusion Our data demonstrate that circulating FH monocytes show differences in cell physiology that may contribute to the early onset of atherosclerosis in this disease.

  6. [Profiles of resilience and quality of life in people with acquired disability due to traffic accidents].

    Science.gov (United States)

    Suriá Martínez, Raquel

    2015-09-01

    To identify distinct profiles of resilience in people with spinal cord injuries due to traffic accidents and to determine whether the profiles identified are related to differences in subjective well-being. The Resilience Scale (Wagnild and Young, 1993) and an adapted quality of life scale (GENCAT) were administered to 98 people with physical disabilities due to traffic accidents. Cluster analyses identified three different resilience profiles: a high-resilience group, a low-resilience group, and a group showing a predominance of high scores in self and life acceptance and social competence. The results also revealed statistically significant differences among profiles in most domains of subjective well-being. The results suggest the need to study resilience in greater depth and to design programs to enhance quality of life among people with disabilities due to traffic accidents. Copyright © 2014 SESPAS. Published by Elsevier Espana. All rights reserved.

  7. Profile Analysis of the Universal Nonverbal Intelligence Test Standardization Sample.

    Science.gov (United States)

    Wilhoit, Brian E.; McCallum, R. Steve

    2002-01-01

    A normative typology was developed and applied using multivariate profile analysis of subtest scores of the Universal Nonverbal Intelligence Test (UNIT) standardization sample. The results yielded a seven-profile cluster solution for the Extended Battery, and a six-profile cluster solution for the Standard Battery. Additionally, the results lend…

  8. Criminal Psychological Profiling

    Science.gov (United States)

    1993-10-18

    landmark report became known to the general population. Dr. Langer’s profile broke new ground. While the practice of psychoanalysis was not new, this marked...school or college dropout. Suspect is probably suffering from one or more forms of paranoid psychosis .6 Perpetrator: Based on this profile, the police

  9. Compton profile of tantalum

    Indian Academy of Sciences (India)

    scale-Compton profile is directly related to the momentum distribution of the electrons. The spectral analysis ... proximation, the Compton profile J(q) is the projection of the target's electron momentum distribution, n(p) ... densed matter and serves as a reliable test of the accuracy of the calculated wave functions. Such basic ...

  10. Reinforced aerodynamic profile

    DEFF Research Database (Denmark)

    2010-01-01

    The present invention relates to the prevention of deformations in an aerodynamic profile caused by lack of resistance to the bending moment forces that are created when such a profile is loaded in operation. More specifically, the invention relates to a reinforcing element inside an aerodynamic...

  11. Chemical profiling of explosives

    NARCIS (Netherlands)

    Brust, G.M.H.

    2014-01-01

    The primary goal of this thesis is to develop analytical methods for the chemical profiling of explosives. Current methodologies for the forensic analysis of explosives focus on identification of the explosive material. However, chemical profiling of explosives becomes increasingly important, as

  12. PROFIL KOMPETENSI DUNIA KERJA BIDANG PERBAIKAN BODI OTOMOTIF DAN TINGKAT RELEVANSINYA DENGAN DUNIA PENDIDIKAN

    Directory of Open Access Journals (Sweden)

    Afri Yudantoko

    2016-08-01

    of TPBO SMK in Bantul. This research was descriptive comparative using the quantitative research approach accompanied by qualitative data as supporting data in the discussion chapter with the survey method. This research involved 42 respondents from BW/IW automotive body repair sector, 7 teachers, and 29 students from TPBO SMK in Bantul. The results showed: (1 there were 147 items of competency in the competency profile of BW/IW automotive body repair sector, (2 there were 85 items of competency contained in the curriculum of TPBO SMK in Bantul and 103 items of competency in the work competency profile implemented in teaching processes at TPBO SMK in Bantul, and (3 the level of relevance between the competency profile of BW/IW automotive body repair sector and the competency profile of the curriculum of TPBO SMK in Bantul was 27.211% (irrelevant. The level of relevance with the work competency profile implemented in teaching processes at TPBO SMK in Bantul was 70.068% (relevant. Keywords: competency profile of world of work of automotive body repair sector, competency profile of TPBO SMK, and relevance

  13. [Fatty acids profile and microstructure of avocado puree after microwave heating].

    Science.gov (United States)

    Guzmán-Gerónimo, Rosa I; Dorantes, Lidia

    2008-09-01

    Changes in the fatty acid profile and the microstructure of avocado puree after microwave treatment were evaluated. The main components of the fatty acid profile were oleic, palmitic, linoleic and palmitoleic acids. Fatty acids profile of microwaved avocado puree did not show significant changes (p puree showed significant changes in its microstructure. Samples treated with microwaves for less than 40 s preserved the cells shape, causing only a minimal modification. On the other hand, microwave treated avocado puree using more than 40 s, showed a disruption of idioblast oil cells, releasing the oil contained on them. The results might be explained based on the sensory evaluation that was performed on the microwaved avocado puree, where samples at 60 s showed oily texture and grassy flavor.

  14. Gene expression profiling during murine tooth development

    Directory of Open Access Journals (Sweden)

    Maria A dos Santos silva Landin

    2012-07-01

    Full Text Available The aim of this study was to describe the expression of genes, including ameloblastin (Ambn, amelogenin X chromosome (Amelx and enamelin (Enam during early (pre-secretory tooth development. The expression of these genes has predominantly been studied at post-secretory stages. Deoxyoligonucleotide microarrays were used to study gene expression during development of the murine first molar tooth germ at 24h intervals, starting at the eleventh embryonic day (E11.5 and up to the seventh day after birth (P7. The profile search function of Spotfire software was used to select genes with similar expression profile as the enamel genes (Ambn, Amelx and Enam. Microarray results where validated using real-time Reverse Transcription-Polymerase Chain Reaction (real-time RT-PCR, and translated proteins identified by Western blotting. In situ localisation of the Ambn, Amelx and Enam mRNAs were monitored from E12.5 to E17.5 using deoxyoligonucleotide probes. Bioinformatics analysis was used to associate biological functions with differentially (p ≤0.05 expressed (DE genes.Microarray results showed a total of 4362 genes including Ambn, Amelx and Enam to be significant differentially expressed throughout the time-course. The expression of the three enamel genes was low at pre-natal stages (E11.5-P0 increasing after birth (P1-P7. Profile search lead to isolation of 87 genes with significantly similar expression to the three enamel proteins. The mRNAs expressed in dental epithelium and epithelium derived cells. Although expression of Ambn, Amelx and Enam were lower during early tooth development compared to secretory stages enamel proteins were detectable by Western blotting. Bioinformatic analysis associated the 87 genes with multiple biological functions. Around thirty-five genes were associated with fifteen transcription factors.

  15. Best in show but not best shape: a photographic assessment of show dog body condition.

    Science.gov (United States)

    Such, Z R; German, A J

    2015-08-01

    Previous studies suggest that owners often wrongly perceive overweight dogs to be in normal condition. The body shape of dogs attending shows might influence owners' perceptions, with online images of overweight show winners having a negative effect. This was an observational in silico study of canine body condition. 14 obese-prone breeds and 14 matched non-obese-probe breeds were first selected, and one operator then used an online search engine to identify 40 images, per breed, of dogs that had appeared at a major national UK show (Crufts). After images were anonymised and coded, a second observer subjectively assessed body condition, in a single sitting, using a previously validated method. Of 1120 photographs initially identified, 960 were suitable for assessing body condition, with all unsuitable images being from longhaired breeds. None of the dogs (0 per cent) were underweight, 708 (74 per cent) were in ideal condition and 252 (26 per cent) were overweight. Pugs, basset hounds and Labrador retrievers were most likely to be overweight, while standard poodles, Rhodesian ridgebacks, Hungarian vizslas and Dobermanns were least likely to be overweight. Given the proportion of show dogs from some breeds that are overweight, breed standards should be redefined to be consistent with a dog in optimal body condition. British Veterinary Association.

  16. Parametric study on shear behaviour for triangular web profile steel section with openings

    Science.gov (United States)

    De'nan, Fatimah; Keong, Choong Kok; Hashim, Nor Salwani; Yan, Kong Jing

    2017-10-01

    The cost of steel section had been the important factor in design process. Hence, triangular web profile which has higher shear capacity than normal flat web had been introduced. In order to reduce the steel weight, opening in web had been introduced. In this paper, a numerical study on shear strength for triangular web profile steel section with opening is carried out. A total of four various opening shapes and sizes of triangular web profile steel section are developed. A comparison of finite element analysis results on triangular web profile steel section with various web openings is reported. Overall results show that in term of deformed shapes, each model of the triangular web profile steel section with openings behave similarly. Triangular web profile steel section without opening has higher ultimate shear strength compared with triangular web profile steel section with openings. The best shape of opening is diamond shape because it's give highest shear buckling capacity compared to circle, square and hexagon shape openings. Reduction in volume for diamond shape is lesser than the reduction in volume for circle, square and hexagon shape openings. The reduction in volume decreases, as the opening size decreases.

  17. Novel knowledge-based mean force potential at the profile level

    Directory of Open Access Journals (Sweden)

    Wang Xiaolong

    2006-06-01

    Full Text Available Abstract Background The development and testing of functions for the modeling of protein energetics is an important part of current research aimed at understanding protein structure and function. Knowledge-based mean force potentials are derived from statistical analyses of interacting groups in experimentally determined protein structures. Current knowledge-based mean force potentials are developed at the atom or amino acid level. The evolutionary information contained in the profiles is not investigated. Based on these observations, a class of novel knowledge-based mean force potentials at the profile level has been presented, which uses the evolutionary information of profiles for developing more powerful statistical potentials. Results The frequency profiles are directly calculated from the multiple sequence alignments outputted by PSI-BLAST and converted into binary profiles with a probability threshold. As a result, the protein sequences are represented as sequences of binary profiles rather than sequences of amino acids. Similar to the knowledge-based potentials at the residue level, a class of novel potentials at the profile level is introduced. We develop four types of profile-level statistical potentials including distance-dependent, contact, Φ/Ψ dihedral angle and accessible surface statistical potentials. These potentials are first evaluated by the fold assessment between the correct and incorrect models generated by comparative modeling from our own and other groups. They are then used to recognize the native structures from well-constructed decoy sets. Experimental results show that all the knowledge-base mean force potentials at the profile level outperform those at the residue level. Significant improvements are obtained for the distance-dependent and accessible surface potentials (5–6%. The contact and Φ/Ψ dihedral angle potential only get a slight improvement (1–2%. Decoy set evaluation results show that the distance

  18. Analytical profile of moxidectin.

    Science.gov (United States)

    Awasthi, Atul; Razzak, Majid; Al-Kassas, Raida; Harvey, Joanne; Garg, Sanjay

    2013-01-01

    Moxidectin or F28249α is a potent endectocide and semisynthetic methoxime derivative of naturally occurring nemadectin. It is well known for the novel mode of action against a broad range of nematode and anthropod animal parasites. In this work, physicochemical and pharmaceutical aspects of moxidectin are described including stability, semisynthesis, purification processes, formulation compositions, impurities, and degradation pathways. Additional experiments such as DSC, XRD, and CHN analysis were carried out to complete the profile of moxidectin. The importance of safety and quality of drug substances was highlighted by chronological developments involving moxidectin and its analogues. The information gathered from the literature was used to trace the origins of moxidectin-related substances presented in the European Pharmacopeia (EP) compendial monograph. During the review, it was noticed that majority of impurities presented in the EP does not have any potential to increase with time in drug substance or formulated products; therefore, they do not require monitoring during stability studies. This also showed the requirement for further characterization of the impurities observed during long-term storage and development of stability indicating methods distinguishing between process impurities and the true degradation products. Furthermore, the stability of moxidectin in formulations is also reviewed in conjunction with known degradation routes and innovative ways to formulate products that are stable and effective at intended shelf life. Copyright © 2013 Elsevier Inc. All rights reserved.

  19. Sensing the wind profile

    DEFF Research Database (Denmark)

    Pena Diaz, Alfredo

    2009-01-01

    This thesis consists of two parts. The first is a synopsis of the theoretical progress of the study that is based on a number of journal papers. The papers, which constitute the second part of the report, aim to analyze, measure, and model the wind prole in and beyond the surface layer by combining...... observations from cup anemometers with lidars. The lidar is necessary to extend the measurements on masts at the Horns Rev offshore wind farm and over at land at Høvsøre, Denmark. Both sensing techniques show a high degree of agreement for wind speed measurements performed at either sites. The wind speed...... measurements are averaged for several stability conditions and compare well with the surface-layer wind profile. At Høvsøre, it is sufficient to scale the wind speed with the surface friction velocity, whereas at Horns Rev a new scaling is added, due to the variant roughness length. This new scaling is coupled...

  20. Aliskiren: a clinical profile

    Directory of Open Access Journals (Sweden)

    Roland E Schmieder

    2006-06-01

    Full Text Available Aliskiren is a novel oral antihypertensive agent, and the first in the new class of direct renin inhibitors. Here we review the key criteria that a new antihypertensive drug should possess, notably effective blood pressure lowering as monotherapy and combination therapy, 24-hour blood pressure control, safety and tolerability, end-organ protective effects, minimal drug interaction and efficacy during long-term use.Aliskiren fulfils key criteria for a new antihypertensive agent.The drug demonstrates effective blood lowering in a number of studies as monotherapy and in combination with a thiazide diuretic (hydrochlorothiazide, an angiotensin-converting enzyme inhibitor (ramipril and a calcium channel blocker (amlodipine. Other studies applying ambulatory blood pressure monitoring show that aliskiren maintains blood pressure control for more than 24 hours. Aliskiren, 150 mg and 300 mg have demonstrated a placebo-like safety and tolerability profile, with no interactions with a wide range of commonly used drugs. Three studies (AVOID, ALOFT and ALLAY are ongoing properties. with aliskiren to assess end-organ protective properties.

  1. Aliskiren: A clinical profile

    Directory of Open Access Journals (Sweden)

    Roland E Schmieder

    2006-06-01

    Full Text Available Aliskiren is a novel oral antihypertensive agent, and the first in the new class of direct renin inhibitors. Here we review the key criteria that a new antihypertensive drug should possess, notably effective blood pressure lowering as monotherapy and combination therapy, 24-hour blood pressure control, safety and tolerability end-organ protective effects, minimal drug interaction and efficacy during long-term use. Aliskiren fulfils key criteria for a new antihypertensive agent. The drug demonstrates effective blood lowering in a number of studies as monotherapy and in combination with a thiazide diuretic (hydrochlorothiazide, an angiotensin-converting enzyme inhibitor (ramipril and a calcium channel blocker (amlodipine. Other studies applying ambulatory blood pressure monitoring show that aliskiren maintains blood pressure control for more than 24 hours. Aliskiren, 150 mg and 300 mg have demonstrated a placebo-like safety and tolerability profile with no interactions with a wide range of commonly used drugs. Three studies (AVOID, ALOFT and ALLAY are ongoing with aliskiren to assess end-organ protective properties.

  2. A Quantitative Profiling Tool for Diverse Genomic Data Types Reveals Potential Associations between Chromatin and Pre-mRNA Processing.

    Science.gov (United States)

    Kremsky, Isaac; Bellora, Nicolás; Eyras, Eduardo

    2015-01-01

    High-throughput sequencing, and genome-based datasets in general, are often represented as profiles centered at reference points to study the association of protein binding and other signals to particular regulatory mechanisms. Although these profiles often provide compelling evidence of these associations, they do not provide a quantitative assessment of the enrichment, which makes the comparison between signals and conditions difficult. In addition, a number of biases can confound profiles, but are rarely accounted for in the tools currently available. We present a novel computational method, ProfileSeq, for the quantitative assessment of biological profiles to provide an exact, nonparametric test that specific regions of the test profile have higher or lower signal densities than a control set. The method is applicable to high-throughput sequencing data (ChIP-Seq, GRO-Seq, CLIP-Seq, etc.) and to genome-based datasets (motifs, etc.). We validate ProfileSeq by recovering and providing a quantitative assessment of several results reported before in the literature using independent datasets. We show that input signal and mappability have confounding effects on the profile results, but that normalizing the signal by input reads can eliminate these biases while preserving the biological signal. Moreover, we apply ProfileSeq to ChIP-Seq data for transcription factors, as well as for motif and CLIP-Seq data for splicing factors. In all examples considered, the profiles were robust to biases in mappability of sequencing reads. Furthermore, analyses performed with ProfileSeq reveal a number of putative relationships between transcription factor binding to DNA and splicing factor binding to pre-mRNA, adding to the growing body of evidence relating chromatin and pre-mRNA processing. ProfileSeq provides a robust way to quantify genome-wide coordinate-based signal. Software and documentation are freely available for academic use at https://bitbucket.org/regulatorygenomicsupf/profileseq/.

  3. The effects of nonuniform illumination and temperature profiles on silicon solar cells under concentrated sunlight

    Science.gov (United States)

    Sanderson, R. W.; Odonnel, D. T.; Backus, C. E.

    The independent effects of either nonuniform illumination or nonuniform temperature distribution on the performance of silicon solar cells under concentrated sunlight are examined. Results from both a theoretical model and experimental observations show that the electrical performance of the concentrator solar cell under nonuniform temperature is dependent on both the magnitude and the location of the nonuniform temperature profile. The model results, however, failed to predict the degree of bending of the open circuit voltage curve which was observed experimentally. For nonuniform illumination, experimental and theoretical results show that both the efficiency and the open circuit voltage are decreased compared with the uniform illumination case. The nonillumination profiles included step changes of illuminated to nonilluminated parts of the cell as well as continuously varying profiles across the geometry of the cell. It is found that the efficiency and the overall voltage of the cell can be increased by illuminating the cell closer to the busbar.

  4. Renewable Energy Country Profiles. Caribbean

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-09-15

    IRENA Renewable Energy Country Profiles take stock of the latest developments in the field of renewables at country level around the world. Each profile combines analysis by IRENA's specialists with the latest available country data and additional information from a wide array of sources. The resulting reports provide a brief yet comprehensive picture of the situation with regard to renewable energy, including energy supply, electrical generation and grid capacity, and access. Energy policies, targets and projects are also considered, along with each country's investment climate and endowment with renewable energy resources. The energy statistics presented here span the period from 2009 until 2012, reflecting varying timelines in the source material. Since data availability differs from country to country, wider regional comparisons are possible only for the latest year with figures available for every country included. Despite the time lag in some cases, the evident differences and disparities between countries and regions around the world remain striking. The current package of country profiles is just a starting point. The geographic scope will continue to expand, and existing profiles will be enhanced with new indicators, with the whole series maintained as a live product on the IRENA website (www.irena.org)

  5. An empirical assessment of content in criminal psychological profiles.

    Science.gov (United States)

    Kocsis, Richard N

    2003-02-01

    Although criminal psychological profiling has been in use by law enforcement agencies for almost three decades, there is a paucity of empirical research examining the technique. A fundamental issue that has received little attention is the empirical evaluation of information contained in profiles composed by professional profilers. In this study, a group of profilers, police officers, psychologists, college students, and self-declared psychics were given information from a solved murder investigation, after which the participants composed a written profile predicting the probable offender. Professional profilers tended to write more lengthy profiles that contained more information about the nonphysical attributes of the offender and more information about the crime scene or the offender's behavior before, during, and after the crime. These results are discussed in terms of their implication for our broader understanding of the technique of profiling and future directions for research into profiling.

  6. Impact of Atmospheric Infrared Sounder (AIRS) Thermodynamic Profiles on Regional Weather Forecasting

    Science.gov (United States)

    Chou, Shih-Hung; Zavodsky, Bradley T.; Jedlovee, Gary J.

    2010-01-01

    In data sparse regions, remotely-sensed observations can be used to improve analyses and lead to better forecasts. One such source comes from the Atmospheric Infrared Sounder (AIRS), which together with the Advanced Microwave Sounding Unit (AMSU), provides temperature and moisture profiles with accuracy comparable to that of radiosondes. The purpose of this paper is to describe a procedure to assimilate AIRS thermodynamic profile data into a regional configuration of the Advanced Research Weather Research and Forecasting (WRF-ARW) model using its three-dimension variational (3DVAR) analysis component (WRF-Var). Quality indicators are used to select only the highest quality temperature and moisture profiles for assimilation in both clear and partly cloudy regions. Separate error characteristics for land and water profiles are also used in the assimilation process. Assimilation results indicate that AIRS profiles produce an analysis closer to in situ observations than the background field. Forecasts from a 37-day case study period in the winter of 2007 show that AIRS profile data can lead to improvements in 6-h cumulative precipitation forecasts due to instability added in the forecast soundings by the AIRS profiles. Additionally, in a convective heavy rainfall event from February 2007, assimilation of AIRS profiles produces a more unstable boundary layer resulting in enhanced updrafts in the model. These updrafts produce a squall line and precipitation totals that more closely reflect ground-based observations than a no AIRS control forecast. The location of available high-quality AIRS profiles ahead of approaching storm systems is found to be of paramount importance to the amount of impact the observations will have on the resulting forecasts.

  7. Profiling the Mobile Customer

    DEFF Research Database (Denmark)

    Jessen, Pernille Wegener; King, Nancy J.

    2010-01-01

    Mobile customers are increasingly being tracked and profiled by behavioural advertisers to enhance delivery of personalized advertising. This type of profiling relies on automated processes that mine databases containing personally-identifying or anonymous consumer data, and it raises a host...... of significant concerns about privacy and data protection. This second article in a two part series on "Profiling the Mobile Customer" explores how to best protect consumers' privacy and personal data through available mechanisms that include industry self-regulation, privacy-enhancing technologies...

  8. A prototype ionization profile monitor for RHIC

    Energy Technology Data Exchange (ETDEWEB)

    Connolly, R.; Cameron, P.; Ryan, W. [and others

    1997-07-01

    Transverse beam profiles in the Relativistic Heavy-Ion Collider (RHIC) will be measured with ionization profile monitors (IPM`s). Each IPM collects and measures the distribution of electrons in the beamline resulting from residual gas ionization during bunch passage. The electrons are swept transversely from the beamline and collected on strip anodes oriented parallel to the beam axis. At each bunch passage the charge pulses are amplified, integrated, and digitized for display as a profile histogram. A prototype detector was tested in the injection line during the RHIC Sextant Test. This paper describes the detector and gives results from the beam tests.

  9. Profile Patterns in the 16PF Questionnaire.

    Science.gov (United States)

    Lorr, Maurice; Suziedelis, Antanas

    1985-01-01

    Four profile clusters were identified and replicated across two or three samples of students and adults and for three cluster analysis methods. The results were compared with those reported by other investigators. (Author/BL)

  10. Refactoring Real-Time Java Profiles

    DEFF Research Database (Denmark)

    Søndergaard, Hans; Thomsen, Bent; Ravn, Anders Peter

    2011-01-01

    it a suitable foundation for the proposed levels of SCJ. The ongoing work of specifying the SCJ profile builds on sub classing of RTSJ. This spurred our interest in a refactoring approach. It starts by extracting the common kernel of the specifications in a core package, which defines interfaces only......Just like other software, Java profiles benefits from refactoring when they have been used and have evolved for some time. This paper presents a refactoring of the Real-Time Specification for Java (RTSJ) and the Safety Critical Java (SCJ) profile (JSR-302). It highlights core concepts and makes....... It is then possible to refactor SCJ with its three levels and RTSJ in such a way that each profile is in a separate package. This refactoring results in cleaner class hierarchies with no superfluous methods, well defined SCJ levels, elimination of SCJ annotations like @SCJAllowed, thus making the profiles easier...

  11. ROMANIAN ENTREPRENEUR PROFILE AND BEHAVIOR

    OpenAIRE

    Lavinia Mirabela JIANU (BORCOS); Oana Mihaela BARA

    2014-01-01

    Due to major contribution of the entrepreneurs to the economic and social development, the knowledge of their main characteristics has a special significance The paper presents the results of a economic research conducted in April and May 2014 and offers an exhaustive approach on the characteristics and behavior of the Romanian entrepreneur, a lot of aspects being analyzed which are not present in other researches.According to this research, the Romanian entrepreneur’s profile is the followin...

  12. OMANIAN ENTREPRENEUR PROFILE AND BEHAVIOR

    OpenAIRE

    Lavinia Mirabela JIANU (BORCOS); Oana Mihaela BARA

    2014-01-01

    Due to major contribution of the entrepreneurs to the economic and social development, the knowledge of their main characteristics has a special significance The paper presents the results of a economic research conducted in April and May 2014 and offers an exhaustive approach on the characteristics and behavior of the Romanian entrepreneur, a lot of aspects being analyzed which are not present in other researches.According to this research, the Romanian entrepreneur’s profile is the followin...

  13. Photometric analysis of esthetically pleasant and unpleasant facial profile

    Directory of Open Access Journals (Sweden)

    Helena Nunes da Rocha Fortes

    2014-04-01

    Full Text Available Objective: To identify which linear, angular and proportionality measures could influence a profile to be considered esthetically pleasant or unpleasant, and to assess sexual dimorphism. Methods: 150 standardized facial profile photographs of dental students of both sexes were obtained and printed on photographic paper. Ten plastic surgeons, ten orthodontists and ten layperson answered a questionnaire characterizing each profile as pleasant, acceptable or unpleasant. With the use of a score system, the 15 most pleasant and unpleasant profiles of each sex were selected. The photographs were scanned into AutoCAD computer software. Linear, angular and proportion measurements were obtained using the software tools. The average values between groups were compared by the Student's t-test and the Mann-Whitney test at 5%. Results: The linear measures LL-S, LL-H, LL-E, LL-B and Pn-H showed statistically significant differences (p < 0.05. Statistical differences were also found in the angular measures G'.Pn.Pg', G'.Sn.Pg' and Sn.Me'.C and in the proportions G'-Sn:Sn-Me' and Sn-Gn':Gn'-C (p < 0.05. Differences between sexes were found for the linear measure Ala-Pn, angles G'-Pg'.N-Pn, Sn.Me'.C, and proportions Gn'-Sn:Sn-Me' and Ala-Pn:N'-Sn. (p < 0.05. Conclusion: The anteroposterior position of the lower lip, the amount of nose that influences the profile, facial convexity, total vertical proportion and lip-chin proportion appear to influence pleasantness of facial profile. Sexual dimorphism was identified in nasal length, nasofacial and lower third of the face angles, total vertical and nasal height/length proportions.

  14. Computer-Assisted Instruction: What the Research Shows.

    Science.gov (United States)

    Bracey, Gerald W.

    1987-01-01

    Briefly reviews current research on the effectiveness of computer-assisted instruction (CAI) and microcomputers. The Hawthorne Effect and research results for elementary, secondary, and college level students are discussed, courseware requirements are described, and research studies showing positive and negative effects of CAI are listed. (LRW)

  15. Auditory temporal-order thresholds show no gender differences

    NARCIS (Netherlands)

    van Kesteren, Marlieke T. R.; Wierslnca-Post, J. Esther C.

    2007-01-01

    Purpose: Several studies on auditory temporal-order processing showed gender differences. Women needed longer inter-stimulus intervals than men when indicating the temporal order of two clicks presented to the left and right ear. In this study, we examined whether we could reproduce these results in

  16. Perturbation of the Hematopoietic Profile by Anabolic Androgenic Steroids

    Directory of Open Access Journals (Sweden)

    Jenny Erkander Mullen

    2014-01-01

    Full Text Available Objective. The aim of this study was to investigate the hematopoietic profile in AAS abusers, during or short after their last abuse and approximately six months later. Moreover, we studied if supraphysiological doses of testosterone influence the concentration of hemoglobin and erythropoietin in healthy volunteers. Design and Methods. Subjects (N=31 were recruited through an antidoping hotline. The hematological profile was measured when the subjects entered the study and approximately 6 months later. Testosterone enanthate (500 mg was administered to healthy volunteers (N=24. Gene expression was studied in human hek293 cells exposed to 1 μM testosterone. Results. Decreased levels of hemoglobin, erythrocyte volume fraction, and erythrocyte counts were observed after 6 months without the use of AAS. Results in volunteers show that hemoglobin increased 3% four and 15 days after testosterone administration, whereas EPO was significantly increased by 38% four days after dose. Agreeingly, in vitro study shows that testosterone induces the mRNA level of EPO with 65% after 24-hour exposure. Conclusion. These results indicate that supraphysiological doses of testosterone may cause a perturbation in the hematopoietic profile. This is of interest in relation to the adverse cardiovascular effects observed in AAS abusers.

  17. Profile Physical Education modules at medical universities

    Directory of Open Access Journals (Sweden)

    Pavlov V.I.

    2011-12-01

    Full Text Available The article states the results of investigation in development and introduction into the medical educational practice of experimental educational modules of profile Physical Education. The research goal is the development and realization of the pedagogical process of medical specialists by means of profiled training modules. More than 1,000 people were involved in the research. The results of the survey assisted in preparing three educational methodical manuals, recommended for the use in the educational process of medical specialists

  18. Food System - Results Profile Nov 2016 ENGLISH.PUB

    International Development Research Centre (IDRC) Digital Library (Canada)

    fdieudonne

    Policies for healthier food environments. • Community healthy food initiatives. • Reducing salt, sugar, and fat. • Food marketing and labelling. Policies for healthier food environments. Food policies and regulations influence the affordability, availability, accessibility, and diversity of healthy foods and shape food choices made ...

  19. Fishing Community Profiles

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — To enable fisheries managers to comply with National Standard 8 (NS8), NMFS social scientists around the nation are preparing fishing community profiles that present...

  20. Wind Profiling Radar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Clutter present in radar return signals as used for wind profiling is substantially removed by carrying out a Daubechies wavelet transformation on a time series of...

  1. Prescription Drug Profiles PUF

    Data.gov (United States)

    U.S. Department of Health & Human Services — This release contains the Prescription Drug Profiles Public Use Files (PUFs) drawn from Medicare prescription drug claims for the year of the date on which the...

  2. Fire Management Species Profiles

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The objective of the Fire Management Species Profile project is to identify habitat management objectives that are specific, measurable, achievable, clearly...

  3. Household electricity demand profiles

    DEFF Research Database (Denmark)

    Marszal, Anna Joanna; Heiselberg, Per Kvols; Larsen, Olena Kalyanova

    2016-01-01

    Highlights •A 1-min resolution household electricity load model is presented. •Model adapts a bottom-up approach with single appliance as the main building block. •Load profiles are used to analyse the flexibility potential of household appliances. •Load profiles can be applied in other domains, e.......g. building energy simulations. •The demand level of houses with different number of occupants is well captured....

  4. Absolute beam emittance measurements at RHIC using ionization profile monitors

    Energy Technology Data Exchange (ETDEWEB)

    Minty, M. [Brookhaven National Lab. (BNL), Upton, NY (United States). Collider-Accelerator Dept.; Connolly, R [Brookhaven National Lab. (BNL), Upton, NY (United States). Collider-Accelerator Dept.; Liu, C. [Brookhaven National Lab. (BNL), Upton, NY (United States). Collider-Accelerator Dept.; Summers, T. [Brookhaven National Lab. (BNL), Upton, NY (United States). Collider-Accelerator Dept.; Tepikian, S. [Brookhaven National Lab. (BNL), Upton, NY (United States). Collider-Accelerator Dept.

    2014-08-15

    In the past, comparisons between emittance measurements obtained using ionization profile monitors, Vernier scans (using as input the measured rates from the zero degree counters, or ZDCs), the polarimeters and the Schottky detectors evidenced significant variations of up to 100%. In this report we present studies of the RHIC ionization profile monitors (IPMs). After identifying and correcting for two systematic instrumental errors in the beam size measurements, we present experimental results showing that the remaining dominant error in beam emittance measurements at RHIC using the IPMs was imprecise knowledge of the local beta functions. After removal of the systematic errors and implementation of measured beta functions, precise emittance measurements result. Also, consistency between the emittances measured by the IPMs and those derived from the ZDCs was demonstrated.

  5. Direct comparisons of GOMOS and SAGE III NO3 vertical profiles

    Directory of Open Access Journals (Sweden)

    J. R. Moore

    2012-07-01

    Full Text Available In this paper, we present the first global comparisons between the two unique satellite-borne data sets of nitrogen trioxide (NO3 vertical profiles retrieved from the GOMOS (Global Ozone Monitoring by the Occultation of Stars stellar occultations and the SAGE III (Stratospheric Aerosol and Gas Experiment lunar occultations. The comparison results indicate that between the altitudes 25 km and 45 km the median difference between these two data sets is within ± 25%. The study of zonal median profiles shows that the climatologies calculated from GOMOS and SAGE III profiles are comparable and represent the same features in all latitude bands. No clear systematic differences are observed. The median profiles are closest in the tropics and slightly deviating at high latitudes.

  6. Development of residual gas ionization profile monitor for high intensity proton beams

    CERN Document Server

    Sato, Y; Hirose, E; Ieiri, M; Igarashi, Y; Inaba, S; Katoh, Y; Minakawa, M; Noumi, H; Saitó, M; Suzuki, Y; Takahashi, H; Takasaki, M; Tanaka, K; Toyoda, A; Yamada, Y; Yamanoi, Y; Watanabe, H

    2006-01-01

    Nondestructive beam profile monitor utilizing ionizations of residual gas has been developed for continuous monitoring of 3?0(J-PARC). Knock-on electrons produced in the ionizations of residual gas vacuumed to 1 Pa are collected with a uniform electric field applied between electrodes. Applying a uniform electric field parallel to the electric field is essential to reduce diffusion of electrons crossing over magnetic flux. A prototype monitor has been constructed and installed in EP2-C beam line at KEK 12 GeV proton synchrotron (12 Ge V-PS). The profiles measured with the present monitor agree with the ones measured with the existing destructive profile monitor. The present monitor shows sufficient performances as a candidate of the profile monitor at J-PARC. In the present article, the working principle of the present monitor, the results of test experiments, and further developments are described in detail.

  7. Identification of multiple intelligences with the Multiple Intelligence Profiling Questionnaire III

    OpenAIRE

    KIRSI TIRRI; PETRI NOKELAINEN

    2008-01-01

    In this study, we present the latest version of the Multiple Intelligences Profiling Questionnaire (MIPQ III) that is based on Howard Gardner’s (e.g., 1983, 1999) MI theory. The operationalization of nine MI scales is tested with an empirical sample of Finnish preadolescents and adults (n = 410). Results of the internal consistency analysis show that the nine MIPQ III dimensions have satisfactory reliability coefficients with the sample. Results of the interscale correlation analysis show tha...

  8. Gender-typed attributes and marital satisfaction among Mexican immigrant couples: A latent profile approach.

    Science.gov (United States)

    Wood, Claire A; Helms, Heather M; Supple, Andrew J; Perlman, Daniel

    2015-06-01

    Informed by socioecological and dyadic approaches to understanding marriage, the current study examined the patterning of gender-typed attributes among 120 Mexican immigrant opposite sex couples and the subsequent links with spouses' reports of marital satisfaction. Latent profile analysis (LPA) was used to identify typologies of couples based on spouses' self-reported masculine and feminine attributes. Three couple profiles were identified: (a) Androgynous Couples, (b) Undifferentiated Couples, and (c) Mismatched Couples. Results from a mixed model ANCOVA showed profile differences in couples' marital satisfaction based on profile membership, suggesting that spouses in the Undifferentiated Profile were the least satisfied. Findings illustrate a lack of gender-typing at the individual and couple levels that challenge stereotypical and patriarchal depictions of Latino marital relationships and propose a more complex understanding of Mexican-origin spouses' gender-typed attributes than has yet been portrayed in the literature. The finding that couples with 1 androgynous partner (i.e., wives in the Mismatched Profile) reported similar levels of marital satisfaction to couples in the Androgynous Profile offers additional insights regarding how these qualities operate under the unique socioecological niches that Mexican immigrant couples inhabit-contexts that may place demands on spouses that challenge gendered and culturally bound depictions of marriage. (c) 2015 APA, all rights reserved).

  9. The virialization density of peaks with general density profiles under spherical collapse

    Energy Technology Data Exchange (ETDEWEB)

    Rubin, Douglas [Department of Physics, Harvard University, Cambridge, MA, 02138 (United States); Loeb, Abraham, E-mail: dsrubin@physics.harvard.edu, E-mail: aloeb@cfa.harvard.edu [Department of Astronomy, Harvard University, Cambridge, MA, 02138 (United States)

    2013-12-01

    We calculate the non-linear virialization density, Δ{sub c}, of halos under spherical collapse from peaks with an arbitrary initial and final density profile. This is in contrast to the standard calculation of Δ{sub c} which assumes top-hat profiles. Given our formalism, the non-linear halo density can be calculated once the shape of the initial peak's density profile and the shape of the virialized halo's profile are provided. We solve for Δ{sub c} for halos in an Einstein de-Sitter and a ΛCDM universe. As examples, we consider power-law initial profiles as well as spherically averaged peak profiles calculated from the statistics of a Gaussian random field. We find that, depending on the profiles used, Δ{sub c} is smaller by a factor of a few to as much as a factor of 10 as compared to the density given by the standard calculation ( ≈ 200). Using our results, we show that, for halo finding algorithms that identify halos through an over-density threshold, the halo mass function measured from cosmological simulations can be enhanced at all halo masses by a factor of a few. This difference could be important when using numerical simulations to assess the validity of analytic models of the halo mass function.

  10. Cannabinoid agonists showing BuChE inhibition as potential therapeutic agents for Alzheimer's disease.

    Science.gov (United States)

    González-Naranjo, Pedro; Pérez-Macias, Natalia; Campillo, Nuria E; Pérez, Concepción; Arán, Vicente J; Girón, Rocio; Sánchez-Robles, Eva; Martín, María Isabel; Gómez-Cañas, María; García-Arencibia, Moisés; Fernández-Ruiz, Javier; Páez, Juan A

    2014-02-12

    Designing drugs with a specific multi-target profile is a promising approach against multifactorial illnesses as Alzheimer's disease. In this work, new indazole ethers that possess dual activity as both cannabinoid agonists CB2 and inhibitors of BuChE have been designed by computational methods. On the basis of this knowledge, the synthesis, pharmacological evaluation and docking studies of a new class of indazoles has been performed. Pharmacological evaluation includes radioligand binding assays with [(3)H]-CP55940 for CB1R and CB2R and functional activity for cannabinoid receptors on isolated tissue. Additionally, in vitro inhibitory assays of AChE/BuChE and the corresponding competition studies have been carried out. The results of pharmacological tests have revealed that three of these derivatives behave as CB2 cannabinoid agonists and simultaneously show BuChE inhibition. In particular, compounds 3 and 24 have emerged as promising candidates as novel cannabinoids that inhibit BuChE by a non-competitive or mixed mechanism, respectively. On the other hand, both molecules show antioxidant properties. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  11. Resultados histológicos e detecção do HPV em mulheres com células escamosas atípicas de significado indeterminado e lesão escamosa intra-epitelial de baixo grau na colpocitologia oncológica Histological results and HPV detection in women with pap smear showing atypical squamous cells of undetermined significance and low-grade squamous intraepithelial lesion

    Directory of Open Access Journals (Sweden)

    André Luis Ferreira Santos

    2004-07-01

    cells of unknown significance (ASCUS or low-grade squamous intraepithelial lesions (LSIL. METHODS: a cross-sectional study comprising 161 women referred to the Taubaté University Hospital due to ASCUS/LSIL, between August 2000 and September 2002. All women responded to a questionnaire regarding sociodemographic and reproductive characteristics and were subjected to gynecological examination with specimen collection for Pap test and HCII, along with colposcopy and eventual cervical biopsy. The relationship between HCII results and age, use of condom, oral hormonal contraception, and smoking were evaluated by the chi-square test. The sensitivity, specificity, positive and negative predictive values of both Pap test and HCII were calculated. All calculations were performed within 95% confidence intervals. RESULTS: sixty-seven percent of the women that tested positive for HPV were less than 30 years old. Pap smear and HCII showed the same 82% sensitivity in detecting CIN2/3 when the threshold for a positive Pap result was ASCUS, LSIL or HSIL. Pap smear specificity and positive predictive values were substantially increased when only HSIL results were considered as positive (from 29 to 95% and 12 to 50%, respectively. These figures were superior to those of HCII, but at the expense of an expressive loss of sensitivity (from 82% to 41%. CONCLUSIONS: our results substantiate the potential of HCII in detecting CIN2/3 among women referred due to ASCUS/LSIL.

  12. The Well-being Profile--an Internet tool for school health promotion.

    Science.gov (United States)

    Lintonen, Tomi; Konu, A I

    2006-01-01

    For years, the WHO has, in accordance with the 'settings' idea, encouraged a whole school approach when trying to promote health and well-being in schools. This developmental study analyses the implementation and pilot phase experiences of a holistic well-being evaluation tool for schools, the School Well-being Profile, on the Internet. The Profile is based on the theoretically established School Well-being Model. The School Well-being Profile consists of electronic survey forms and an automatic facility that analyses and produces results on the data in graphic and numeric form. After the data has been entered, the primary user within the school can immediately view and print out the results. The figures can be compared with the averages of all schools to pinpoint areas where well-being is different from that in the other schools. The Profile resides on a Finnish National Board of Education server (www2.edu.fi/hyvinvointiprofiili) and its use is free of charge for all schools. The Profile became popular in its first year: it was used by 33 primary schools, 28 lower secondary schools and 9 upper secondary schools with a total of 9,169 respondents. Overall, 94% of the students and 99% of the personnel expressed that it had been at least fairly easy to fill in the questionnaire. The paper shows that theoretical research in health promotion can effectively be put into practice using information technology tools. The project that produced the School Well-being Profile ended in 2004, yet the WWW-Profile continues to gain new users. The school administrators, personnel and students have found the Profile easy to use and worth the time and effort invested by the school. The strategies adopted in the design and dissemination of the Profile seem to have been successful in providing a sustainable resource to contribute to the future promotion of well-being in schools.

  13. Affective profiles in Italian high school students: life satisfaction, psychological well-being, self-esteem, and optimism

    Directory of Open Access Journals (Sweden)

    Annamaria eDi Fabio

    2015-09-01

    Full Text Available The affective profiles model distinguishes between individuals who are self-fulfilling (high positive affect, low negative affect, high affective (high positive affect, high negative affect, low affective (low positive affect, low negative affect, and self-destructive (low positive affect, high negative affect. The literature shows that the affective profiles model has been used with Swedish people in particular in order to determine differences among profiles in relation to life satisfaction, psychological well-being, self-esteem, and optimism. The present research investigated these differences in Italian high school students. Two studies were conducted: the first with 156 Italian high school students and the second with 148 Italian high school students. The first study analyzed differences among affective profiles with regard to life satisfaction and psychological well-being while the second study analyzed differences among affective profiles with regard to self-esteem and optimism. In the first study, the Positive and Negative Affect Schedule (PANAS, the Satisfaction With Life Scale (SWLS, and the Meaningful Life Measure (MLM were administered to the participants. In the second study, the Positive and Negative Affect Schedule (PANAS, the Self-Esteem Scale (SES, the Life Orientation Test - revised (LOT-r were administered to the participants. The results of the first study showed that, with respect to the other profiles, the self-fulfilling participants had greater life satisfaction and psychological well-being. The results of the second study showed that, with respect to the other profiles, the self-fulfilling participants had higher self-esteem and optimism. These results revealed differences among affective profiles regarding life satisfaction, psychological well-being, self-esteem, and optimism in the Italian context as well thereby offering new possibilities for cross-cultural research and for enhancing self-fulfilling profiles.

  14. Affective profiles in Italian high school students: life satisfaction, psychological well-being, self-esteem, and optimism.

    Science.gov (United States)

    Di Fabio, Annamaria; Bucci, Ornella

    2015-01-01

    The affective profiles model distinguishes between individuals who are self-fulfilling (high positive affect, low negative affect), high affective (high positive affect, high negative affect), low affective (low positive affect, low negative affect), and self-destructive (low positive affect, high negative affect). The literature shows that the affective profiles model has been used with Swedish people in particular in order to determine differences among profiles in relation to life satisfaction, psychological well-being, self-esteem, and optimism. The present research investigated these differences in Italian high school students. Two studies were conducted: the first with 156 Italian high school students and the second with 148 Italian high school students. The first study analyzed differences among affective profiles with regard to life satisfaction and psychological well-being while the second study analyzed differences among affective profiles with regard to self-esteem and optimism. In the first study, the Positive and Negative Affect Schedule (PANAS), the Satisfaction with Life Scale, and the Meaningful Life Measure were administered to the participants. In the second study, the PANAS, the Self-Esteem Scale, the Life Orientation Test-revised were administered to the participants. The results of the first study showed that, with respect to the other profiles, the self-fulfilling participants had greater life satisfaction and psychological well-being. The results of the second study showed that, with respect to the other profiles, the self-fulfilling participants had higher self-esteem and optimism. These results revealed differences among affective profiles regarding life satisfaction, psychological well-being, self-esteem, and optimism in the Italian context as