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Sample records for profiler suite omps

  1. NOAA JPSS Ozone Mapping and Profiler Suite (OMPS) Nadir Profile Science Sensor Data Record (SDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Ozone Mapping and Profiler Suite (OMPS) onboard the Suomi-NPP satellite monitors ozone from space. OMPS will collect total column and vertical profile ozone data...

  2. NOAA JPSS Ozone Mapping and Profiler Suite (OMPS) Nadir Total Column Sensor Data Record (SDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Ozone Mapping and Profiler Suite (OMPS) onboard the Suomi NPP satellite monitors ozone from space. OMPS will collect total column and vertical profile ozone data...

  3. NOAA JPSS Ozone Mapping and Profiler Suite (OMPS) Version 8 Nadir Profile Ozone (V8Pro) Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of nadir profiler ozone from the Ozone Mapping and Profiling Suite (OMPS) instrument...

  4. NOAA JPSS Ozone Mapping and Profiler Suite (OMPS) Version 8 Total Ozone (V8TOz) Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of total column ozone from the Ozone Mapping and Profiling Suite (OMPS) instrument...

  5. New-Generation NASA Aura Ozone Monitoring Instrument (OMI) Volcanic SO2 Dataset: Algorithm Description, Initial Results, and Continuation with the Suomi-NPP Ozone Mapping and Profiler Suite (OMPS)

    Science.gov (United States)

    Li, Can; Krotkov, Nickolay A.; Carn, Simon; Zhang, Yan; Spurr, Robert J. D.; Joiner, Joanna

    2017-01-01

    coarser spatial and spectral resolution of the Suomi National Polar-orbiting Partnership (Suomi-NPP) Ozone Mapping and Profiler Suite (OMPS) instrument, application of the new PCA algorithm to OMPS data produces highly consistent retrievals between OMI and OMPS. The new PCA algorithm is therefore capable of continuing the volcanic SO2 data record well into the future using current and future hyperspectral UV satellite instruments.

  6. Retrieval of ozone profiles from OMPS limb scattering observations

    Directory of Open Access Journals (Sweden)

    C. Arosio

    2018-04-01

    Full Text Available This study describes a retrieval algorithm developed at the University of Bremen to obtain vertical profiles of ozone from limb observations performed by the Ozone Mapper and Profiler Suite (OMPS. This algorithm is based on the technique originally developed for use with data from the SCanning Imaging Absorption spectroMeter for Atmospheric CHartographY (SCIAMACHY instrument. As both instruments make limb measurements of the scattered solar radiation in the ultraviolet (UV and visible (Vis spectral ranges, an underlying objective of the study is to obtain consolidated and consistent ozone profiles from the two satellites and to produce a combined data set. The retrieval algorithm uses radiances in the UV and Vis wavelength ranges normalized to the radiance at an upper tangent height to obtain ozone concentrations in the altitude range of 12–60 km. Measurements at altitudes contaminated by clouds in the instrument field of view are identified and filtered out. An independent aerosol retrieval is performed beforehand and its results are used to account for the stratospheric aerosol load in the ozone inversion. The typical vertical resolution of the retrieved profiles varies from  ∼  2.5 km at lower altitudes ( < 30 km to  ∼  1.5 km (about 45 km and becomes coarser at upper altitudes. The retrieval errors resulting from the measurement noise are estimated to be 1–4 % above 25 km, increasing to 10–30 % in the upper troposphere. OMPS data are processed for the whole of 2016. The results are compared with the NASA product and validated against profiles derived from passive satellite observations or measured in situ by balloon-borne sondes. Between 20 and 60 km, OMPS ozone profiles typically agree with data from the Microwave Limb Sounder (MLS v4.2 within 5–10 %, whereas in the lower altitude range the bias becomes larger, especially in the tropics. The comparison of OMPS profiles with ozonesonde

  7. Retrieval of ozone profiles from OMPS limb scattering observations

    Science.gov (United States)

    Arosio, Carlo; Rozanov, Alexei; Malinina, Elizaveta; Eichmann, Kai-Uwe; von Clarmann, Thomas; Burrows, John P.

    2018-04-01

    This study describes a retrieval algorithm developed at the University of Bremen to obtain vertical profiles of ozone from limb observations performed by the Ozone Mapper and Profiler Suite (OMPS). This algorithm is based on the technique originally developed for use with data from the SCanning Imaging Absorption spectroMeter for Atmospheric CHartographY (SCIAMACHY) instrument. As both instruments make limb measurements of the scattered solar radiation in the ultraviolet (UV) and visible (Vis) spectral ranges, an underlying objective of the study is to obtain consolidated and consistent ozone profiles from the two satellites and to produce a combined data set. The retrieval algorithm uses radiances in the UV and Vis wavelength ranges normalized to the radiance at an upper tangent height to obtain ozone concentrations in the altitude range of 12-60 km. Measurements at altitudes contaminated by clouds in the instrument field of view are identified and filtered out. An independent aerosol retrieval is performed beforehand and its results are used to account for the stratospheric aerosol load in the ozone inversion. The typical vertical resolution of the retrieved profiles varies from ˜ 2.5 km at lower altitudes ( passive satellite observations or measured in situ by balloon-borne sondes. Between 20 and 60 km, OMPS ozone profiles typically agree with data from the Microwave Limb Sounder (MLS) v4.2 within 5-10 %, whereas in the lower altitude range the bias becomes larger, especially in the tropics. The comparison of OMPS profiles with ozonesonde measurements shows differences within ±5 % between 13 and 30 km at northern middle and high latitudes. At southern middle and high latitudes, an agreement within 5-7 % is also achieved in the same altitude range. An unexpected bias of approximately 10-20 % is detected in the lower tropical stratosphere. The processing of the 2013 data set using the same retrieval settings and its validation against ozonesondes reveals a much

  8. The OMPS Limb Profiler Instrument: Two-Dimensional Retrieval Algorithm

    Science.gov (United States)

    Rault, Didier F.

    2010-01-01

    The upcoming Ozone Mapper and Profiler Suite (OMPS), which will be launched on the NPOESS Preparatory Project (NPP) platform in early 2011, will continue monitoring the global distribution of the Earth's middle atmosphere ozone and aerosol. OMPS is composed of three instruments, namely the Total Column Mapper (heritage: TOMS, OMI), the Nadir Profiler (heritage: SBUV) and the Limb Profiler (heritage: SOLSE/LORE, OSIRIS, SCIAMACHY, SAGE III). The ultimate goal of the mission is to better understand and quantify the rate of stratospheric ozone recovery. The focus of the paper will be on the Limb Profiler (LP) instrument. The LP instrument will measure the Earth's limb radiance (which is due to the scattering of solar photons by air molecules, aerosol and Earth surface) in the ultra-violet (UV), visible and near infrared, from 285 to 1000 nm. The LP simultaneously images the whole vertical extent of the Earth's limb through three vertical slits, each covering a vertical tangent height range of 100 km and each horizontally spaced by 250 km in the cross-track direction. Measurements are made every 19 seconds along the orbit track, which corresponds to a distance of about 150km. Several data analysis tools are presently being constructed and tested to retrieve ozone and aerosol vertical distribution from limb radiance measurements. The primary NASA algorithm is based on earlier algorithms developed for the SOLSE/LORE and SAGE III limb scatter missions. All the existing retrieval algorithms rely on a spherical symmetry assumption for the atmosphere structure. While this assumption is reasonable in most of the stratosphere, it is no longer valid in regions of prime scientific interest, such as polar vortex and UTLS regions. The paper will describe a two-dimensional retrieval algorithm whereby the ozone distribution is simultaneously retrieved vertically and horizontally for a whole orbit. The retrieval code relies on (1) a forward 2D Radiative Transfer code (to model limb

  9. Post-launch performance evaluation of the OMPS Nadir Mapper and Nadir Profiler

    Science.gov (United States)

    Grotenhuis, Michael G.; Wu, Xiangqian; Flynn, Larry; Beach, Eric; Niu, Jianguo; Yu, Wei

    2014-09-01

    The Joint Polar Satellite System (JPSS) represents the latest generation of polar-orbiting satellites operated by the National Oceanic and Atmospheric Administration (NOAA). The first in the JPSS series of satellites, the Suomi National Polar-orbiting Partnership (NPP) spacecraft was launched in November 2011 to bridge the gap between the current Polar Operational Environmental Satellites (POES) and the future JPSS-1. The Ozone Mapping Profiler Suite (OMPS) is a suite of hyperspectral instruments onboard the Suomi NPP spacecraft designed to continue atmospheric ozone records through both atmospheric profiles and global distribution mapping. OMPS will also be included on the future JPSS payloads. In order to properly extend measurements from previous ozone instruments, including the Solar Backscatter Ultraviolet (SBUV) instrument on POES, proper OMPS calibration is necessary. In this study, the postlaunch performance of the OMPS Nadir Mapper (NM) and Nadir Profiler (NP) are evaluated through their Sensor Data Records (SDRs), which validates their end-to-end calibration. This is achieved through stability monitoring and intercomparison.

  10. Expected Performance of Ozone Climate Data Records from Ozone Mapping and Profiler Suite Limb Profiler

    Science.gov (United States)

    Xu, P. Q.; Rault, D. F.; Pawson, S.; Wargan, K.; Bhartia, P. K.

    2012-01-01

    The Ozone Mapping and Profiler Suite Limb Profiler (OMPS/LP) was launched on board of the Soumi NPP space platform in late October 2011. It provides ozone-profiling capability with high-vertical resolution from 60 Ian to cloud top. In this study, an end-to-end Observing System Simulation Experiment (OSSE) of OMPS/LP ozone is discussed. The OSSE was developed at NASA's Global Modeling and Assimilation Office (GMAO) using the Goddard Earth Observing System (GEOS-5) data assimilation system. The "truth" for this OSSE is built by assimilating MLS profiles and OMI ozone columns, which is known to produce realistic three-dimensional ozone fields in the stratosphere and upper troposphere. OMPS/LP radiances were computed at tangent points computed by an appropriate orbital model. The OMPS/LP forward RT model, Instrument Models (IMs) and EDR retrieval model were introduced and pseudo-observations derived. The resultant synthetic OMPS/LP observations were evaluated against the "truth" and subsequently these observations were assimilated into GEOS-5. Comparison of this assimilated dataset with the "truth" enables comparisons of the likely uncertainties in 3-D analyses of OMPS/LP data. This study demonstrated the assimilation capabilities of OMPS/LP ozone in GEOS-5, with the monthly, zonal mean (O-A) smaller than 0.02ppmv at all levels, the nns(O-A) close to O.lppmv from 100hPa to 0.2hPa; and the mean(O-B) around the 0.02ppmv for all levels. The monthly zonal mean analysis generally agrees to within 2% of the truth, with larger differences of 2-4% (0.1-0.2ppmv) around 10hPa close to North Pole and in the tropical tropopause region, where the difference is above 20% due to the very low ozone concentrations. These OSSEs demonstrated that, within a single data assimilation system and the assumption that assimilated MLS observations provide a true rendition of the stratosphere, the OMPS/LP ozone data are likely to produce accurate analyses through much of the stratosphere

  11. A New Radiometric Calibration Paradigm for the OMPS Nadir Total Column and Profile Instruments

    Science.gov (United States)

    Heath, Donald; Georgiew, Georgi

    2011-01-01

    A fused silica Mie Scattering Diffuser (MSD) has been developed at Ball Aerospace & Technology Corp. that has measured characteristics which could be used to increase the accuracy of the spectral albedo calibration of the Ozone Mapping and Profiler Suite (OMPS) Nadir ozone total column and profile instrument by almost an order of magnitude. Measurements have been made of the optical characteristics on both natural and synthetic forms of fused silica MSDs. Preliminary measurements suggest that MSDs are useable in the solar reflective wavelength region from 250 nm to 3.7 m. To date synthetic and natural MSDs have been irradiated for 60 hours of UV radiation from a solar simulator, and synthetic MSDs have been irradiated with increasing doses of Co-60 gamma rays at 30, 500 krads up to 1.5 Mrads, and 30 krads of 200 MeV protons. The principal effects have been small loses in transmittance at wavelengths < 350 nm. The high energy particle irradiation measurements were provided by Neal Nickles and Dean Spieth.

  12. Validation of ozone profile retrievals derived from the OMPS LP version 2.5 algorithm against correlative satellite measurements

    Science.gov (United States)

    Kramarova, Natalya A.; Bhartia, Pawan K.; Jaross, Glen; Moy, Leslie; Xu, Philippe; Chen, Zhong; DeLand, Matthew; Froidevaux, Lucien; Livesey, Nathaniel; Degenstein, Douglas; Bourassa, Adam; Walker, Kaley A.; Sheese, Patrick

    2018-05-01

    The Limb Profiler (LP) is a part of the Ozone Mapping and Profiler Suite launched on board of the Suomi NPP satellite in October 2011. The LP measures solar radiation scattered from the atmospheric limb in ultraviolet and visible spectral ranges between the surface and 80 km. These measurements of scattered solar radiances allow for the retrieval of ozone profiles from cloud tops up to 55 km. The LP started operational observations in April 2012. In this study we evaluate more than 5.5 years of ozone profile measurements from the OMPS LP processed with the new NASA GSFC version 2.5 retrieval algorithm. We provide a brief description of the key changes that had been implemented in this new algorithm, including a pointing correction, new cloud height detection, explicit aerosol correction and a reduction of the number of wavelengths used in the retrievals. The OMPS LP ozone retrievals have been compared with independent satellite profile measurements obtained from the Aura Microwave Limb Sounder (MLS), Atmospheric Chemistry Experiment Fourier Transform Spectrometer (ACE-FTS) and Odin Optical Spectrograph and InfraRed Imaging System (OSIRIS). We document observed biases and seasonal differences and evaluate the stability of the version 2.5 ozone record over 5.5 years. Our analysis indicates that the mean differences between LP and correlative measurements are well within required ±10 % between 18 and 42 km. In the upper stratosphere and lower mesosphere (> 43 km) LP tends to have a negative bias. We find larger biases in the lower stratosphere and upper troposphere, but LP ozone retrievals have significantly improved in version 2.5 compared to version 2 due to the implemented aerosol correction. In the northern high latitudes we observe larger biases between 20 and 32 km due to the remaining thermal sensitivity issue. Our analysis shows that LP ozone retrievals agree well with the correlative satellite observations in characterizing vertical, spatial and temporal

  13. Merged SAGE II, Ozone_cci and OMPS ozone profile dataset and evaluation of ozone trends in the stratosphere

    Directory of Open Access Journals (Sweden)

    V. F. Sofieva

    2017-10-01

    Full Text Available In this paper, we present a merged dataset of ozone profiles from several satellite instruments: SAGE II on ERBS, GOMOS, SCIAMACHY and MIPAS on Envisat, OSIRIS on Odin, ACE-FTS on SCISAT, and OMPS on Suomi-NPP. The merged dataset is created in the framework of the European Space Agency Climate Change Initiative (Ozone_cci with the aim of analyzing stratospheric ozone trends. For the merged dataset, we used the latest versions of the original ozone datasets. The datasets from the individual instruments have been extensively validated and intercompared; only those datasets which are in good agreement, and do not exhibit significant drifts with respect to collocated ground-based observations and with respect to each other, are used for merging. The long-term SAGE–CCI–OMPS dataset is created by computation and merging of deseasonalized anomalies from individual instruments. The merged SAGE–CCI–OMPS dataset consists of deseasonalized anomalies of ozone in 10° latitude bands from 90° S to 90° N and from 10 to 50 km in steps of 1 km covering the period from October 1984 to July 2016. This newly created dataset is used for evaluating ozone trends in the stratosphere through multiple linear regression. Negative ozone trends in the upper stratosphere are observed before 1997 and positive trends are found after 1997. The upper stratospheric trends are statistically significant at midlatitudes and indicate ozone recovery, as expected from the decrease of stratospheric halogens that started in the middle of the 1990s and stratospheric cooling.

  14. Validation of ozone profile retrievals derived from the OMPS LP version 2.5 algorithm against correlative satellite measurements

    Directory of Open Access Journals (Sweden)

    N. A. Kramarova

    2018-05-01

    Full Text Available The Limb Profiler (LP is a part of the Ozone Mapping and Profiler Suite launched on board of the Suomi NPP satellite in October 2011. The LP measures solar radiation scattered from the atmospheric limb in ultraviolet and visible spectral ranges between the surface and 80 km. These measurements of scattered solar radiances allow for the retrieval of ozone profiles from cloud tops up to 55 km. The LP started operational observations in April 2012. In this study we evaluate more than 5.5 years of ozone profile measurements from the OMPS LP processed with the new NASA GSFC version 2.5 retrieval algorithm. We provide a brief description of the key changes that had been implemented in this new algorithm, including a pointing correction, new cloud height detection, explicit aerosol correction and a reduction of the number of wavelengths used in the retrievals. The OMPS LP ozone retrievals have been compared with independent satellite profile measurements obtained from the Aura Microwave Limb Sounder (MLS, Atmospheric Chemistry Experiment Fourier Transform Spectrometer (ACE-FTS and Odin Optical Spectrograph and InfraRed Imaging System (OSIRIS. We document observed biases and seasonal differences and evaluate the stability of the version 2.5 ozone record over 5.5 years. Our analysis indicates that the mean differences between LP and correlative measurements are well within required ±10 % between 18 and 42 km. In the upper stratosphere and lower mesosphere (> 43 km LP tends to have a negative bias. We find larger biases in the lower stratosphere and upper troposphere, but LP ozone retrievals have significantly improved in version 2.5 compared to version 2 due to the implemented aerosol correction. In the northern high latitudes we observe larger biases between 20 and 32 km due to the remaining thermal sensitivity issue. Our analysis shows that LP ozone retrievals agree well with the correlative satellite observations in characterizing

  15. SUIT

    DEFF Research Database (Denmark)

    Algreen-Ussing, Gregers; Wedebrunn, Ola

    2003-01-01

    Leaflet om project SUIT udgivet af European Commission. Tryksagen forklarer i korte ord resultatet af projektet SUIT. Kulturværdier i Miljøspørgsmål. Vurdering af projekter og indvirkning på miljø....

  16. Profiling the SO2 Plume from Volcan Turrialba: Ticosonde Balloon Measurements Compared with OMI and OMPS Retrievals

    Science.gov (United States)

    Selkirk, H. B.; Krotkov, N. A.; Li, C.; Morris, G.; Diaz, J. A.; Carn, S. A.; Voemel, H.; Nord, P. M.; Larson, K.

    2014-12-01

    The summit of Volcan Turrialba (elev. 3340 m) lies less than 50 km upstream in the prevailing easterlies from the Ticosonde balloon launch site at San Jose, Costa Rica, where ECC ozone sondes have been launched regularly since 2005. In 2006 we began to see telltale notches in the ozone profiles in the altitude range between 2 and 6 km. Given the proximity of Turrialba, it seemed likely that SO2 in the volcano's plume was interfering in the chemical reaction in the ECC ozone sonde used to detect ozone. In early 2010, fumarolic activity in the Turrialba crater increased strongly, and the profile notches in our soundings increased in frequency as well, consistent with this hypothesis. In February 2012 we tested a dual ECC sonde system, where an additional sonde is flown on the same payload using a selective SO2 filter. The difference of the measurements in the dual sonde is a direct measure of the amount of SO2 encountered. This first dual sonde passed through the plume, and the data indicated a tropospheric SO2 column of 1.4 DU, comparing favorably with a total column of 1.7 DU in the OMI 3-km linear fit (LF) product at the sonde profile location and at nearly the same time. We are now launching dual sondes on a regular basis with 18 launches in the first 12 months through July 2014; 11 of these have detectable SO2 signals. These soundings have great potential for validation of the Aura OMI and the Suomi-NPP OMPS retrievals of SO2. Here we present the sonde measurements and compare them with two satellite datasets: the Aura OMI Linear Fit (LF) product and the Suomi-NPP OMPS Principal Components Analysis (PCA) boundary layer product. The PCA algorithm reduces retrieval noise and artifacts by more accurately accounting for various interferences in SO2 retrievals such as O3 absorption and rotational Raman scattering. The comparisons with the in situ observations indicate a significant improvement of the PCA algorithm in capturing relatively weak volcanic SO2 signals.

  17. Profiling the SO2 Plume from Volcan Turrialba: Ticosonde Balloon Measurements Compared with OMI and OMPS Retrievals

    Science.gov (United States)

    Selkirk, Henry; Krotkov, Nickolay; Li, Can; Morris, Gary (Inventor); Diaz, Jorge Andres; Carn, Simon; Vomel, Holger; Corrales, Ernesto; Nord, Paul; Larson, Kelsey

    2014-01-01

    The summit of Volcan Turrialba (elev. 3340 m) lies less than 50 km upstream in the prevailing easterlies from the Ticosonde balloon launch site at San Jose, Costa Rica, where ECC ozone sondes have been launched regularly since 2005. In 2006 we began to see telltale notches in the ozone profiles in the altitude range between 2 and 6 km. Given the proximity of Turrialba, it seemed likely that SO2 in the volcano's plume was interfering in the chemical reaction in the ECC ozone sonde used to detect ozone. In early 2010, fumarolic activity in the Turrialba crater increased strongly, and the profile notches in our soundings increased in frequency as well, consistent with this hypothesis. In February 2012 we tested a dual ECC sonde system, where an additional sonde is flown on the same payload using a selective SO2 filter. The difference of the measurements in the dual sonde is a direct measure of the amount of SO2 encountered. This first dual sonde passed through the plume, and the data indicated a tropospheric SO2 column of 1.4 DU, comparing favorably with a total column of 1.7 DU in the OMI 3-km linear fit (LF) product at the sonde profile location and at nearly the same time. We are now launching dual sondes on a regular basis with 18 launches in the first 12 months through July 2014; 11 of these have detectable SO2 signals. These soundings have great potential for validation of the Aura OMI and the Suomi-NPP OMPS retrievals of SO2. Here we present the sonde measurements and compare them with two satellite datasets: the Aura OMI Linear Fit (LF) product and the Suomi-NPP OMPS Principal Components Analysis (PCA) boundary layer product. The PCA algorithm reduces retrieval noise and artifacts by more accurately accounting for various interferences in SO2 retrievals such as O3 absorption and rotational Raman scattering. The comparisons with the in situ observations indicate a significant improvement of the PCA algorithm in capturing relatively weak volcanic SO2 signals.

  18. OMPS Near Real-time Products Available Through NASA LANCE (Land Atmosphere Near Real-time Capability for EOS)

    Science.gov (United States)

    Warnock, A.; Durbin, P. B.; Cechini, M. F.; Masuoka, E.

    2017-12-01

    Near real-time (NRT) images from the NASA Ozone Mapping and Profiler Suite (OMPS) for sulfur dioxide, total column ozone and aerosol index products are now available through NASA's online Land Atmosphere Near real-time Capability for EOS (LANCE) system. Color palettes, image dimensions and data ranges have been aligned with the corresponding OMI products, allowing for direct comparison of OMPS NRT images with OMI NRT images already available in NASA Worldview. The images are delivered to LANCE within hours of satellite observation. LANCE NRT imagery can be interactively viewed through Worldview and the Global Imagery Browse Services (GIBS).

  19. Aerosol layer height from synergistic use of VIIRS and OMPS

    Science.gov (United States)

    Lee, J.; Hsu, N. Y. C.; Sayer, A. M.; Kim, W.; Seftor, C. J.

    2017-12-01

    This study presents an Aerosol Single-scattering albedo and Height Estimation (ASHE) algorithm, which retrieves the height of UV-absorbing aerosols by synergistically using the Visible Infrared Imaging Radiometer Suite (VIIRS) and the Ozone Mapping and Profiler Suite (OMPS). ASHE provides height information over a much broader area than ground-based or spaceborne lidar measurements by benefitting from the wide swaths of the two instruments used. As determination of single-scattering albedo (SSA) of the aerosol layer is the most critical part for the performance and coverage of ASHE, here we demonstrate three different strategies to constrain the SSA. First, ASHE is able to retrieve the SSA of UV-absorbing aerosols when Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) provides vertical profiles of the aerosol layer of interest. Second, Aerosol Robotic Network (AERONET) inversions can directly constrain the SSA of the aerosol layer when collocated with VIIRS or OMPS. Last, a SSA climatology from ASHE, AERONET, or other data sources can be used for large-scale, aged aerosol events, for which climatological SSA is well-known, at the cost of a slight decrease in retrieval accuracy. The same algorithm can be applied to measurements of similar type, such as those made by the Moderate Resolution Imaging Spectroradiometer (MODIS) and Ozone Monitoring Instrument (OMI), for a long-term, consistent data record.

  20. A New Algorithm for Detecting Cloud Height using OMPS/LP Measurements

    Science.gov (United States)

    Chen, Zhong; DeLand, Matthew; Bhartia, Pawan K.

    2016-01-01

    The Ozone Mapping and Profiler Suite Limb Profiler (OMPS/LP) ozone product requires the determination of cloud height for each event to establish the lower boundary of the profile for the retrieval algorithm. We have created a revised cloud detection algorithm for LP measurements that uses the spectral dependence of the vertical gradient in radiance between two wavelengths in the visible and near-IR spectral regions. This approach provides better discrimination between clouds and aerosols than results obtained using a single wavelength. Observed LP cloud height values show good agreement with coincident Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation (CALIPSO) measurements.

  1. Using MERRA-2 analysis fields to simulate limb scattered radiance profiles for inhomogeneous atmospheric lines of sight: Preparation for data assimilation of OMPS LP radiances through 2D single-scattering GSLS radiative transfer model development

    Science.gov (United States)

    Loughman, R. P.; Bhartia, P. K.; Moy, L.; Kramarova, N. A.; Wargan, K.

    2016-12-01

    Many remote sensing techniques used to monitor the Earth's upper atmosphere fall into the broad category of "limb viewing" (LV) measurements, which includes any method for which the line of sight (LOS) fails to intersect the surface. Occultation, limb emission and limb scattering (LS) measurements are all LV methods that offer strong sensitivity to changes in the atmosphere near the tangent point of the LOS, due to the enhanced geometric path through the tangent layer (where the concentration also typically peaks, for most atmospheric species). But many of the retrieval algorithms used to interpret LV measurements assume that the atmosphere consists of "spherical shells", in which the atmospheric properties vary only with altitude (creating a 1D atmosphere). This assumption simplifies the analysis, but at the possible price of misinterpreting measurements made in the real atmosphere. In this presentation, we focus on the problem of LOS inhomogeneity for LS measurements made by the OMPS Limb Profiler (LP) instrument during the 2015 ozone hole period. The GSLS radiative transfer model (RTM) used in the default OMPS LP algorithms assumes a spherical-shell atmosphere defined at levels spaced 1 km apart, with extinction coefficients assumed to vary linearly with height between levels. Several recent improvements enable an updated single-scattering version of the GSLS RTM to ingest 3D MERRA-2 analysis fields (including temperature, pressure, and ozone concentration) when creating the model atmosphere, by introducing flexible altitude grids, flexible atmospheric specification along the LOS, and improved treatment of the radiative transfer within each atmospheric layer. As a result, the effect of LOS inhomogeneity on the current (1D) OMPS LP retrieval algorithm can now be studied theoretically, using realistic 3D atmospheric profiles. This work also represents a step towards enabling OMPS LP data to be ingested as part of future data assimilation efforts.

  2. Volcanic Ash and SO2 Monitoring Using Suomi NPP Direct Broadcast OMPS Data

    Science.gov (United States)

    Seftor, C. J.; Krotkov, N. A.; McPeters, R. D.; Li, J. Y.; Brentzel, K. W.; Habib, S.; Hassinen, S.; Heinrichs, T. A.; Schneider, D. J.

    2014-12-01

    NASA's Suomi NPP Ozone Science Team, in conjunction with Goddard Space Flight Center's (GSFC's) Direct Readout Laboratory, developed the capability of processing, in real-time, direct readout (DR) data from the Ozone Mapping and Profiler Suite (OMPS) to perform SO2 and Aerosol Index (AI) retrievals. The ability to retrieve this information from real-time processing of DR data was originally developed for the Ozone Monitoring Instrument (OMI) onboard the Aura spacecraft and is used by Volcano Observatories and Volcanic Ash Advisory Centers (VAACs) charged with mapping ash clouds from volcanic eruptions and providing predictions/forecasts about where the ash will go. The resulting real-time SO2 and AI products help to mitigate the effects of eruptions such as the ones from Eyjafjallajokull in Iceland and Puyehue-Cordón Caulle in Chile, which cause massive disruptions to airline flight routes for weeks as airlines struggle to avoid ash clouds that could cause engine failure, deeply pitted windshields impossible to see through, and other catastrophic events. We will discuss the implementation of real-time processing of OMPS DR data by both the Geographic Information Network of Alaska (GINA) and the Finnish Meteorological Institute (FMI), which provide real-time coverage over some of the most congested airspace and over many of the most active volcanoes in the world, and show examples of OMPS DR processing results from recent volcanic eruptions.

  3. The Use of OMPS Near Real Time Products in Volcanic Cloud Risk Mitigation and Smoke/Dust Air Quality Assessments

    Science.gov (United States)

    Seftor, C. J.; Krotkov, N. A.; McPeters, R. D.; Li, J. Y.; Durbin, P. B.

    2015-12-01

    Near real time (NRT) SO2 and aerosol index (AI) imagery from Aura's Ozone Monitoring Instrument (OMI) has proven invaluable in mitigating the risk posed to air traffic by SO2 and ash clouds from volcanic eruptions. The OMI products, generated as part of NASA's Land, Atmosphere Near real-time Capability for EOS (LANCE) NRT system and available through LANCE and both NOAA's NESDIS and ESA's Support to Aviation Control Service (SACS) portals, are used to monitor the current location of volcanic clouds and to provide input into Volcanic Ash (VA) advisory forecasts. NRT products have recently been developed using data from the Ozone Mapping and Profiler Suite onboard the Suomi NPP platform; they are currently being made available through the SACS portal and will shortly be incorporated into the LANCE NRT system. We will show examples of the use of OMPS NRT SO2 and AI imagery to monitor recent volcanic eruption events. We will also demonstrate the usefulness of OMPS AI imagery to detect and track dust storms and smoke from fires, and how this information can be used to forecast their impact on air quality in areas far removed from their source. Finally, we will show SO2 and AI imagery generated from our OMPS Direct Broadcast data to highlight the capability of our real time system.

  4. Analysis of Block OMP using Block RIP

    OpenAIRE

    Wang, Jun; Li, Gang; Zhang, Hao; Wang, Xiqin

    2011-01-01

    Orthogonal matching pursuit (OMP) is a canonical greedy algorithm for sparse signal reconstruction. When the signal of interest is block sparse, i.e., it has nonzero coefficients occurring in clusters, the block version of OMP algorithm (i.e., Block OMP) outperforms the conventional OMP. In this paper, we demonstrate that a new notion of block restricted isometry property (Block RIP), which is less stringent than standard restricted isometry property (RIP), can be used for a very straightforw...

  5. Selecting a Benchmark Suite to Profile High-Performance Computing (HPC) Machines

    Science.gov (United States)

    2014-11-01

    architectures. Machines now contain central processing units (CPUs), graphics processing units (GPUs), and many integrated core ( MIC ) architecture all...evaluate the feasibility and applicability of a new architecture just released to the market . Researchers are often unsure how available resources will...architectures. Having a suite of programs running on different architectures, such as GPUs, MICs , and CPUs, adds complexity and technical challenges

  6. A computationally efficient OMP-based compressed sensing reconstruction for dynamic MRI

    International Nuclear Information System (INIS)

    Usman, M; Prieto, C; Schaeffter, T; Batchelor, P G; Odille, F; Atkinson, D

    2011-01-01

    Compressed sensing (CS) methods in MRI are computationally intensive. Thus, designing novel CS algorithms that can perform faster reconstructions is crucial for everyday applications. We propose a computationally efficient orthogonal matching pursuit (OMP)-based reconstruction, specifically suited to cardiac MR data. According to the energy distribution of a y-f space obtained from a sliding window reconstruction, we label the y-f space as static or dynamic. For static y-f space images, a computationally efficient masked OMP reconstruction is performed, whereas for dynamic y-f space images, standard OMP reconstruction is used. The proposed method was tested on a dynamic numerical phantom and two cardiac MR datasets. Depending on the field of view composition of the imaging data, compared to the standard OMP method, reconstruction speedup factors ranging from 1.5 to 2.5 are achieved. (note)

  7. First Observations of SO2 from the Satellite Suomi NPP OMPS: Widespread Air Pollution Events Over China

    Science.gov (United States)

    Yang, Kai; Dickerson, Russell R.; Carn, Simon A.; Ge, Cui; Wang, Jun

    2013-01-01

    Severe smog episodes over China in January 2013 received worldwide attention. This air pollution was distinguished by heavy loadings of fine particulate matter and SO2. To characterize these episodes, we employed the Ozone Mapping and Profiler Suite, Nadir Mapper (OMPS NM), an ultraviolet (UV) spectrometer flying on the Suomi National Polar-orbiting Partnership (SNPP) spacecraft since October 2011. We developed an advanced algorithm to quantify SO2 in the lower troposphere and achieved high-quality retrievals from OMPS NM, which are characterized by high precision, approx. 0.2 Dobson Units (DU; 1 DU = 2.69 x 10(exp 16) molecules/sq cm) for instantaneous field of view SO2 data and low biases (within +/-0.2 DU). Here we report SO2 retrievals and UV aerosol index data for these pollution events. The SO2 columns and the areas covered by high pollutant concentrations are quantified; the results reveal for the first time the full extent (an area of approx. 10(exp 6) sq km containing up to 60 kt of SO2) of these episodes.

  8. Outer membrane protein A (OmpA: a new player in shigella flexneri protrusion formation and inter-cellular spreading.

    Directory of Open Access Journals (Sweden)

    Cecilia Ambrosi

    Full Text Available Outer membrane protein A (OmpA is a multifaceted predominant outer membrane protein of Escherichia coli and other Enterobacteriaceae whose role in the pathogenesis of various bacterial infections has recently been recognized. Here, the role of OmpA on the virulence of Shigella flexneri has been investigated. An ompA mutant of wild-type S. flexneri 5a strain M90T was constructed (strain HND92 and it was shown to be severely impaired in cell-to-cell spreading since it failed to plaque on HeLa cell monolayers. The lack of OmpA significantly reduced the levels of IcsA while the levels of cell associated and released IcsP-cleaved 95 kDa amino-terminal portion of the mature protein were similar. Nevertheless, the ompA mutant displayed IcsA exposed across the entire bacterial surface. Surprisingly, the ompA mutant produced proper F-actin comet tails, indicating that the aberrant IcsA exposition at bacterial lateral surface did not affect proper activation of actin-nucleating proteins, suggesting that the absence of OmpA likely unmasks mature or cell associated IcsA at bacterial lateral surface. Moreover, the ompA mutant was able to invade and to multiply within HeLa cell monolayers, although internalized bacteria were found to be entrapped within the host cell cytoplasm. We found that the ompA mutant produced significantly less protrusions than the wild-type strain, indicating that this defect could be responsible of its inability to plaque. Although we could not definitely rule out that the ompA mutation might exert pleiotropic effects on other S. flexneri genes, complementation of the ompA mutation with a recombinant plasmid carrying the S. flexneri ompA gene clearly indicated that a functional OmpA protein is required and sufficient for proper IcsA exposition, plaque and protrusion formation. Moreover, an independent ompA mutant was generated. Since we found that both mutants displayed identical virulence profile, these results further supported the

  9. Satellite Observations of Tropospheric BrO over Salt Lakes and Northern High Latitudes from EOS/OMI and SNPP/OMPS

    Science.gov (United States)

    Kurosu, T. P.; Stutz, J.; Brockway, N.; Saiz-Lopez, A.; Suleiman, R. M.; Natraj, V.; Jaross, G.; Seftor, C. J.

    2017-12-01

    We present observations of tropospheric bromine monoxide (BrO) derived from two satellite instruments: the Ozone Monitoring Instrument (OMI) on EOS-Aura, and the Nadir Mapper component of the Ozone Mapping and Profiler Suite (OMPS) on Suomi/NPP. BrO observations from OMPS constitute a new and experimental measurement that we first report on here and compare with the standard BrO data product from OMI. BrO is a halogen oxide present mostly in the lower stratosphere, where it catalytically destroys ozone with about 25 times the efficiency of ClO. BrO also has a tropospheric component, where it is released from sea surfaces, at the interface of ocean water and sea ice in the polar spring, in volcanic plumes, and in the vicinity of salt lakes. Tropospheric BrO has been linked to mercury (Hg) deposition through BrO-induced conversion of gaseous Hg to reactive Hg, which is then deposited on the surface and enters the food chain, ultimately affecting human health. As part of NASA's Aura Science Team, we are developing an OMI Tropospheric BrO data product that provides a unique global data set on BrO spatial and vertical distribution in the troposphere and stratosphere. Information of this kind is currently unavailable from any of the past and present bromine-monitoring instruments. In this presentation, we focus on multi-year time series of BrO released from a range of salt lakes - the Rann of Kutch, Salar de Uyuni, the Aral Sea, and others. We quantify the amount of bromine released from the lakes and investigate the possibility of lake desiccation monitoring based on independent BrO observations. The quality and limits of OMI and OMPS tropospheric BrO observations is investigated by comparison with ground-based MAX-DOAS observations over central Greenland.

  10. A new software suite for NO2 vertical profile retrieval from ground-based zenith-sky spectrometers

    International Nuclear Information System (INIS)

    Denis, L.; Roscoe, H.K.; Chipperfield, M.P.; Roozendael, M. van; Goutail, F.

    2005-01-01

    Here we present an operational method to improve accuracy and information content of ground-based measurements of stratospheric NO 2 . The motive is to improve the investigation of trends in NO 2 , and is important because the current trend in NO 2 appears to contradict the trend in its source, suggesting that the stratospheric circulation has changed. To do so, a new software package for retrieving NO 2 vertical profiles from slant columns measured by zenith-sky spectrometers has been created. It uses a Rodgers optimal linear inverse method coupled with a radiative transfer model for calculations of transfer functions between profiles and columns, and a chemical box model for taking into account the NO 2 variations during twilight and during the day. Each model has parameters that vary according to season and location. Forerunners of each model have been previously validated. The scheme maps random errors in the measurements and systematic errors in the models and their parameters on to the retrieved profiles. Initialisation for models is derived from well-established climatologies. The software has been tested by comparing retrieved profiles to simultaneous balloon-borne profiles at mid-latitudes in spring

  11. A new software suite for NO{sub 2} vertical profile retrieval from ground-based zenith-sky spectrometers

    Energy Technology Data Exchange (ETDEWEB)

    Denis, L. [British Antarctic Survey/NERC, Madingley Road, Cambridge CB3 0ET (United Kingdom); Roscoe, H.K. [British Antarctic Survey/NERC, Madingley Road, Cambridge CB3 0ET (United Kingdom)]. E-mail: h.roscoe@bas.ac.uk; Chipperfield, M.P. [Environment Centre, University of Leeds, Leeds LS2 9JT (United Kingdom); Roozendael, M. van [Belgian Institute for Space Aeronomy (BIRA/IASB), 1180 Brussels (Belgium); Goutail, F. [Service d' Aeronomie du CNRS, BP3, 91271 Verrieres le Buisson (France)

    2005-05-15

    Here we present an operational method to improve accuracy and information content of ground-based measurements of stratospheric NO{sub 2}. The motive is to improve the investigation of trends in NO{sub 2}, and is important because the current trend in NO{sub 2} appears to contradict the trend in its source, suggesting that the stratospheric circulation has changed. To do so, a new software package for retrieving NO{sub 2} vertical profiles from slant columns measured by zenith-sky spectrometers has been created. It uses a Rodgers optimal linear inverse method coupled with a radiative transfer model for calculations of transfer functions between profiles and columns, and a chemical box model for taking into account the NO{sub 2} variations during twilight and during the day. Each model has parameters that vary according to season and location. Forerunners of each model have been previously validated. The scheme maps random errors in the measurements and systematic errors in the models and their parameters on to the retrieved profiles. Initialisation for models is derived from well-established climatologies. The software has been tested by comparing retrieved profiles to simultaneous balloon-borne profiles at mid-latitudes in spring.

  12. BvrR/BvrS-Controlled Outer Membrane Proteins Omp3a and Omp3b Are Not Essential for Brucella abortus Virulence▿

    Science.gov (United States)

    Manterola, Lorea; Guzmán-Verri, Caterina; Chaves-Olarte, Esteban; Barquero-Calvo, Elías; de Miguel, María-Jesús; Moriyón, Ignacio; Grilló, María-Jesús; López-Goñi, Ignacio; Moreno, Edgardo

    2007-01-01

    The Brucella abortus two-component regulatory system BvrR/BvrS controls the expression of outer membrane proteins (Omp) Omp3a (Omp25) and Omp3b (Omp22). Disruption of bvrS or bvrR generates avirulent mutants with altered cell permeability, higher sensitivity to microbicidal peptides, and complement. Consequently, the role of Omp3a and Omp3b in virulence was examined. Similar to bvrS or bvrR mutants, omp3a and omp3b mutants displayed increased attachment to cells, indicating surface alterations. However, they showed unaltered permeability; normal expression of Omp10, Omp16, Omp19, Omp2b, and Omp1; native hapten polysaccharide; and lipopolysaccharide and were resistant to complement and polymyxin B at ranges similar to those of the wild-type (WT) counterpart. Likewise, omp3a and omp3b mutants were able to replicate in murine macrophages and in HeLa cells, were resistant to the killing action of human neutrophils, and persisted in mice, like the WT strain. Murine macrophages infected with the omp3a mutant generated slightly higher levels of tumor necrosis factor alpha than the WT, whereas the bvrS mutant induced lower levels of this cytokine. Since the absence of Omp3a or Omp3b does not result in attenuation, it can be concluded that BvrR/BvrS influences additional Brucella properties involved in virulence. Our results are discussed in the light of previous works suggesting that disruption of omp3a generates attenuated Brucella strains, and we speculate on the role of group 3 Omps. PMID:17664262

  13. The TOMS V9 Algorithm for OMPS Nadir Mapper Total Ozone: An Enhanced Design That Ensures Data Continuity

    Science.gov (United States)

    Haffner, D. P.; McPeters, R. D.; Bhartia, P. K.; Labow, G. J.

    2015-12-01

    The TOMS V9 total ozone algorithm will be applied to the OMPS Nadir Mapper instrument to supersede the exisiting V8.6 data product in operational processing and re-processing for public release. Becuase the quality of the V8.6 data is already quite high, enchancements in V9 are mainly with information provided by the retrieval and simplifcations to the algorithm. The design of the V9 algorithm has been influenced by improvements both in our knowledge of atmospheric effects, such as those of clouds made possible by studies with OMI, and also limitations in the V8 algorithms applied to both OMI and OMPS. But the namesake instruments of the TOMS algorithm are substantially more limited in their spectral and noise characterisitics, and a requirement of our algorithm is to also apply the algorithm to these discrete band spectrometers which date back to 1978. To achieve continuity for all these instruments, the TOMS V9 algorithm continues to use radiances in discrete bands, but now uses Rodgers optimal estimation to retrieve a coarse profile and provide uncertainties for each retrieval. The algorithm remains capable of achieving high accuracy results with a small number of discrete wavelengths, and in extreme cases, such as unusual profile shapes and high solar zenith angles, the quality of the retrievals is improved. Despite the intended design to use limited wavlenegths, the algorithm can also utilitze additional wavelengths from hyperspectral sensors like OMPS to augment the retreival's error detection and information content; for example SO2 detection and correction of Ring effect on atmospheric radiances. We discuss these and other aspects of the V9 algorithm as it will be applied to OMPS, and will mention potential improvements which aim to take advantage of a synergy with OMPS Limb Profiler and Nadir Mapper to further improve the quality of total ozone from the OMPS instrument.

  14. Isolation of OmpA gene from Salmonella typhimurium and ...

    African Journals Online (AJOL)

    Isolation of OmpA gene from Salmonella typhimurium and transformation into alfalfa in order to develop an edible plant based vaccine. ... The recombinant OmpA was expressed in Escherichia coli TG1. The new construct was used to transform the Agrobacterium tumefaciens Strain LBA4404 before plant transformation.

  15. Loop residues and catalysis in OMP synthase

    DEFF Research Database (Denmark)

    Wang, Gary P.; Hansen, Michael Riis; Grubmeyer, Charles

    2012-01-01

    binding of OMP or PRPP in binary complexes was affected little by loop mutation, suggesting that the energetics of ground-state binding have little contribution from the catalytic loop, or that a favorable binding energy is offset by costs of loop reorganization. Pre-steady-state kinetics for mutants...... values for all four substrate molecules. The 20% (i.e., 1.20) intrinsic [1?-3H]OMP kinetic isotope effect (KIE) for WT is masked because of high forward and reverse commitment factors. K103A failed to express intrinsic KIEs fully (1.095 ± 0.013). In contrast, H105A, which has a smaller catalytic lesion...... (preceding paper in this issue, DOI 10.1021/bi300083p)]. The full expression of KIEs by H105A and E107A may result from a less secure closure of the catalytic loop. The lower level of expression of the KIE by K103A suggests that in these mutant proteins the major barrier to catalysis is successful closure...

  16. Structural and dynamical properties of the porins OmpF and OmpC: insights from molecular simulations

    International Nuclear Information System (INIS)

    Kumar, Amit; Hajjar, Eric; Ruggerone, Paolo; Ceccarelli, Matteo

    2010-01-01

    In this paper we investigate the structural and dynamical properties of the two major porins (OmpF and OmpC) in Escherichia coli, using molecular dynamics (MD) simulations. In particular we characterized the atomic fluctuations, correlated motions, temperature dependence, solvent-accessible cross-sectional area and water dynamics in the key regions of the two channels. Our in-depth analysis allows us to highlight the importance of both the key conserved and substituted residues between OmpF and OmpC. The latter is characterized by a narrower and longer constriction region with respect to OmpF. OmpC also showed a higher stability upon increasing temperature. We then present the results of transport properties by using accelerated MD simulations to probe the diffusion of norfloxacin (a fluoroquinolone antibiotic) through the two porins OmpF/OmpC. Our study constitutes a step forward towards understanding the structure-function relationship of the two porins' channels. This will benefit the research of antibacterials with improved permeation properties and nanopores that aim to use these porins as sensing systems.

  17. Describing Compton scattering and two-quanta positron annihilation based on Compton profiles: Two models suited for the Monte Carlo method

    CERN Document Server

    Bohlen, TT; Patera, V; Sala, P R

    2012-01-01

    An accurate description of the basic physics processes of Compton scattering and positron annihilation in matter requires the consideration of atomic shell structure effects and, in specific, the momentum distributions of the atomic electrons. Two algorithms which model Compton scattering and two-quanta positron annihilation at rest accounting for shell structure effects are proposed. Two-quanta positron annihilation is a physics process which is of particular importance for applications such as positron emission tomography (PET). Both models use a detailed description of the processes which incorporate consistently Doppler broadening and binding effects. This together with the relatively low level of complexity of the models makes them particularly suited to be employed by fast sampling methods for Monte Carlo particle transport. Momentum distributions of shell electrons are obtained from parametrized one-electron Compton profiles. For conduction electrons, momentum distributions are derived in the framework...

  18. Modelling aluminium wire bond reliability in high power OMP devices

    NARCIS (Netherlands)

    Kregting, R.; Yuan, C.A.; Xiao, A.; Bruijn, F. de

    2011-01-01

    In a RF power application such as the OMP, the wires are subjected to high current (because of the high power) and high temperature (because of the heat from IC and joule-heating from the wire itself). Moreover, the wire shape is essential to the RF performance. Hence, the aluminium wire is

  19. Cell envelope proteins of environmental Vibrio cholerae non O1 isolates from Albufera Lake (Valencia, Spain) influence of some factors on OMP expression.

    Science.gov (United States)

    Amaro, C; Herrero, E; Arnau, A; Garay, E

    1989-11-01

    The cell envelope proteins of 89 environmental Vibrio cholerae non O1 strains isolated from lake and coastal waters near Valencia, Spain, and six Vibrio cholerae strains from culture collections were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Considerable heterogeneity was found in the major proteins of the environmental non-O1 strains, but bands between 25,000 and 48,000 daltons were observed in the majority of the strains. Estimated relative mobilities of the total protein profile ranged between 11 and more than 100 Kd. Cluster analysis revealed four groups of strains distinguishable by presence or absence of high and low molecular weight proteins. After treatment with Sarkosyl, the outer membrane proteins (OMPs) were characterized in all strains by densitometric methods. They ranged from 19 to 87 Kilodaltons, and corresponded to the major proteins observed in the total membrane preparations. The major OMP most frequently found had a molecular weight around 37 Kd, similar to that of porins in other Gram-negative bacteria. The OMP composition varied in response to culture medium and growth phase. Generally the OMP expression was affected only in a quantitative way by the growth phase while the growth medium had both a qualitative and a quantitative effect.

  20. Functional assessment of EnvZ/OmpR two-component system in Shewanella oneidensis.

    Directory of Open Access Journals (Sweden)

    Jie Yuan

    Full Text Available EnvZ and OmpR constitute the bacterial two-component signal transduction system known to mediate osmotic stress response in a number of gram-negative bacteria. In an effort to understand the mechanism through which Shewanella oneidensis senses and responds to environmental osmolarity changes, structure of the ompR-envZ operon was determined with Northern blotting assay and roles of the EnvZ/OmpR two-component system in response to various stresses were investigated with mutational analysis, quantitative reverse transcriptase PCR (qRT-PCR, and phenotype microarrays. Results from the mutational analysis and qRT-PCR suggested that the EnvZ/OmpR system contributed to osmotic stress response of S. oneidensis and very likely engaged a similar strategy employed by E. coli, which involved reciprocal regulation of two major porin coding genes. Additionally, the ompR-envZ system was also found related to cell motility. We further showed that the ompR-envZ dependent regulation of porin genes and motility resided almost completely on ompR and only partially on envZ, indicating additional mechanisms for OmpR phosphorylation. In contrast to E. coli lacking ompR-envZ, however, growth of S. oneidensis did not show a significant dependence on ompR-envZ even under osmotic stress. Further analysis with phenotype microarrays revealed that the S. oneidensis strains lacking a complete ompR-envZ system displayed hypersensitivities to a number of agents, especially in alkaline environment. Taken together, our results suggest that the function of the ompR-envZ system in S. oneidensis, although still connected with osmoregulation, has diverged considerably from that of E. coli. Additional mechanism must exist to support growth of S. oneidensis under osmotic stress.

  1. Software Application Profile: RVPedigree: a suite of family-based rare variant association tests for normally and non-normally distributed quantitative traits.

    Science.gov (United States)

    Oualkacha, Karim; Lakhal-Chaieb, Lajmi; Greenwood, Celia Mt

    2016-04-01

    RVPedigree (Rare Variant association tests in Pedigrees) implements a suite of programs facilitating genome-wide analysis of association between a quantitative trait and autosomal region-based genetic variation. The main features here are the ability to appropriately test for association of rare variants with non-normally distributed quantitative traits, and also to appropriately adjust for related individuals, either from families or from population structure and cryptic relatedness. RVPedigree is available as an R package. The package includes calculation of kinship matrices, various options for coping with non-normality, three different ways of estimating statistical significance incorporating triaging to enable efficient use of the most computationally-intensive calculations, and a parallelization option for genome-wide analysis. The software is available from the Comprehensive R Archive Network [CRAN.R-project.org] under the name 'RVPedigree' and at [https://github.com/GreenwoodLab]. It has been published under General Public License (GPL) version 3 or newer. © The Author 2016; all rights reserved. Published by Oxford University Press on behalf of the International Epidemiological Association.

  2. Instant Spring Tool Suite

    CERN Document Server

    Chiang, Geoff

    2013-01-01

    Filled with practical, step-by-step instructions and clear explanations for the most important and useful tasks. A tutorial guide that walks you through how to use the features of Spring Tool Suite using well defined sections for the different parts of Spring.Instant Spring Tool Suite is for novice to intermediate Java developers looking to get a head-start in enterprise application development using Spring Tool Suite and the Spring framework. If you are looking for a guide for effective application development using Spring Tool Suite, then this book is for you.

  3. Molecular identification of the ompL1 gene within Leptospira interrogans standard serovars.

    Science.gov (United States)

    Dezhbord, Mehrangiz; Esmaelizad, Majid; Khaki, Pejvak; Fotohi, Fariba; Zarehparvar Moghaddam, Athena

    2014-06-11

    Leptospirosis, caused by infection with pathogenic Leptospira species, is one of the most prevalent zoonotic diseases in the world. Current leptospiral vaccines are mainly multivalent dead whole-cell mixtures made of several local dominant serovars. Therefore, design and construction of an efficient recombinant vaccine for leptospirosis control is very important. OmpL1 is an immunogenic porin protein that could be of special significance in vaccination and serodiagnosis for leptospirosis. Three strains belonging to pathogenic L. interrogans were analyzed. The specific primers for proliferation of the ompL1 gene were designed. The amplified gene was cloned. In order to investigate the ompL1 nucleotide sequence and homological analysis of this gene, ompL1 genes cloned from standard vaccinal Leptospira serovars prevalent in Iran were sequenced and cloned. PCR amplification of the ompL1 gene using the designed primers resulted in a 963 bp ompL1 gene product. The PCR based on the ompL1 gene detected all pathogenic reference serovars of Leptospira spp. tested. Based on alignment and phylogenetic analysis, although the ompL1 nucleotide sequence was slightly different within three vaccinal serovars (100%-85% identity), amino acid alignment of the OmpL1 proteins revealed that there would be inconsiderable difference among them. The ompL1 gene of the three isolates was well conserved, differing only by a total of 6 bp and the proteins by 2 amino acids. The cloned gene could be further used for expression and recombinant OmpL1 as an efficient and conserved antigen, and may be a useful vaccine candidate against leptospirosis in our region.

  4. Iron is a signal for Stenotrophomonas maltophilia biofilm formation, oxidative stress response, OMPs expression and virulence

    Directory of Open Access Journals (Sweden)

    Carlos Adrian Garcia

    2015-09-01

    Full Text Available Stenotrophomonas maltophilia is an emerging nosocomial pathogen. In many bacteria iron availability regulates, trough the Fur system, not only iron homeostasis but also virulence. The aim of this work was to assess the role of iron on S. maltophilia biofilm formation, EPS production, oxidative stress response, OMPs regulation, quorum sensing (QS, and virulence. Studies were done on K279 and its isogenic fur mutant F60 cultured in the presence or absence of dipyridyl. This is the first report of spontaneous fur mutants obtained in S. maltophilia. F60 produced higher amounts of biofilms than K279a and CLSM analysis demonstrated improved adherence and biofilm organization. Under iron restricted conditions, K279a produced biofilms with more biomass and enhanced thickness. In addition, F60 produced higher amounts of EPS than K279a but with a similar composition, as revealed by ATR-FTIR spectroscopy. With respect to the oxidative stress response, MnSOD was the only SOD isoenzyme detected in K279a. F60 presented higher SOD activity than the wt strain in planktonic and biofilm cultures, and iron deprivation increased K279a SOD activity. Under iron starvation, SDS-PAGE profile from K279a presented two iron-repressed proteins. Mass spectrometry analysis revealed homology with FepA and another putative TonB-dependent siderophore receptor of K279a. In silico analysis allowed the detection of potential Fur boxes in the respective coding genes. K279a encodes the QS diffusible signal factor (DSF. Under iron restriction K279a produced higher amounts of DSF than under iron rich condition. Finally, F60 was more virulent than K279a in the Galleria mellonella killing assay. These results put in evidence that iron levels regulate, likely through the Fur system, S. maltophilia biofilm formation, oxidative stress response, OMPs expression, DSF production and virulence.

  5. Validation suite for MCNP

    International Nuclear Information System (INIS)

    Mosteller, Russell D.

    2002-01-01

    Two validation suites, one for criticality and another for radiation shielding, have been defined and tested for the MCNP Monte Carlo code. All of the cases in the validation suites are based on experiments so that calculated and measured results can be compared in a meaningful way. The cases in the validation suites are described, and results from those cases are discussed. For several years, the distribution package for the MCNP Monte Carlo code1 has included an installation test suite to verify that MCNP has been installed correctly. However, the cases in that suite have been constructed primarily to test options within the code and to execute quickly. Consequently, they do not produce well-converged answers, and many of them are physically unrealistic. To remedy these deficiencies, sets of validation suites are being defined and tested for specific types of applications. All of the cases in the validation suites are based on benchmark experiments. Consequently, the results from the measurements are reliable and quantifiable, and calculated results can be compared with them in a meaningful way. Currently, validation suites exist for criticality and radiation-shielding applications.

  6. Pharmacy settles suit.

    Science.gov (United States)

    1998-10-02

    A suit was filed by an HIV-positive man against a pharmacy that inadvertently disclosed his HIV status to his ex-wife and children. His ex-wife tried to use the information in a custody battle for their two children. The suit against the pharmacy was settled, but the terms of the settlement remain confidential.

  7. Comparison of ompP5 sequence-based typing and pulsed-filed gel ...

    African Journals Online (AJOL)

    In this study, comparison of the outer membrane protein P5 gene (ompP5) sequence-based typing with pulsed-field gel electrophoresis (PFGE) for the genotyping of Haemophilus parasuis, the 15 serovar reference strains and 43 isolates were investigated. When comparing the two methods, 31 ompP5 sequence types ...

  8. The Role of OmpK35, OmpK36 Porins, and Production of β-Lactamases on Imipenem Susceptibility in Klebsiella pneumoniae Clinical Isolates, Cairo, Egypt.

    Science.gov (United States)

    Wassef, Mona; Abdelhaleim, Mona; AbdulRahman, Eiman; Ghaith, Doaa

    2015-12-01

    OmpK35 and OmpK36 are the major outer membrane porins of Klebsiella pneumoniae. We aimed to study the effect of combined porin loss and production of extended-spectrum β-lactamases (ESBLs) on imipenem susceptibility among K. pneumoniae clinical isolates. This study included 91 suspected ESBL-producing K. pneumoniae clinical isolates, isolated from different patient specimens at the Cairo University hospital from January to June 2010. All isolates were subjected to genotypic analysis of the outer membrane protein gene expression using reverse transcription-PCR (RT-PCR) and analysis of OmpK35/36 of 38 isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). By RT-PCR, loss of Omp35 was detected in 78 (85.7%) isolates, loss of Omp36 was detected in 64 (70.32%), and loss of both porins was detected in 62 (68.1%). Out of 91 isolates, 45 (49.5%) were resistant to cefoxitin, and 17 (18.7%) were confirmed as derepressed AmpC producers. Omp35 was lost in all FOX-resistant isolates, whereas Omp36 was lost in 42 (93.3%) (p-value 0.002). The mean of ceftazidime inhibition zone diameter was significantly decreased among ESBL-producing isolates with loss of Omp35/36 (p-value 0.041 and 0.006), respectively. The mean of imipenem minimal inhibitory concentration (MIC) was markedly increased to 8.55 μg/ml among AmpC-producing isolates with Omp35/36 loss, while the mean of imipenem MIC among the 66 confirmed ESBL producers was 0.32 μg/ml. Imipenem MIC was markedly increased among K. pneumoniae isolates showing AmpC production with loss of both porins OmpK35/36. Meanwhile, the association of porin OmpK35/36 loss with ESBL production was not a direct cause of resistance to imipenem.

  9. Fragrance material review on anisyl alcohol (o-m-p-).

    Science.gov (United States)

    Scognamiglio, J; Jones, L; Letizia, C S; Api, A M

    2012-09-01

    A toxicologic and dermatologic review of anisyl alcohol (o-m-p-) when used as a fragrance ingredient is presented. Anisyl alcohol (o-m-p-) is a member of the fragrance structural group Aryl Alkyl Alcohols and is a primary alkyl alcohol. The AAAs are a structurally diverse class of fragrance ingredients that includes primary, secondary, and tertiary alkyl alcohols covalently bonded to an aryl (Ar) group, which may be either a substituted or unsubstituted benzene ring. The common structural element for the AAA fragrance ingredients is an alcohol group -C-(R1)(R2)OH and generically the AAA fragrances can be represented as an Ar-C-(R1)(R2)OH or Ar(-)Alkyl-C-(R1)(R2)OH group. This review contains a detailed summary of all available toxicology and dermatology papers that are related to this individual fragrance ingredient and is not intended as a stand-alone document. A safety assessment of the entire Aryl Alkyl Alcohols will be published simultaneously with this document; please refer to Belsito et al. (2012) for an overall assessment of the safe use of this material and all other branched chain saturated alcohols in fragrances. Copyright © 2011 Elsevier Ltd. All rights reserved.

  10. The host outer membrane proteins OmpA and OmpC are associated with the Shigella phage Sf6 virion

    International Nuclear Information System (INIS)

    Zhao Haiyan; Sequeira, Reuben D.; Galeva, Nadezhda A.; Tang Liang

    2011-01-01

    Assembly of dsDNA bacteriophage is a precisely programmed process. Potential roles of host cell components in phage assembly haven't been well understood. It was previously reported that two unidentified proteins were present in bacteriophage Sf6 virion (Casjens et al, 2004, J.Mol.Biol. 339, 379-394, Fig. 2A). Using tandem mass spectrometry, we have identified the two proteins as outer membrane proteins (OMPs) OmpA and OmpC from its host Shigella flexneri. The transmission electron cryo-microscopy structure of Sf6 shows significant density at specific sites at the phage capsid inner surface. This density fit well with the characteristic beta-barrel domains of OMPs, thus may be due to the two host proteins. Locations of this density suggest a role in Sf6 morphogenesis reminiscent of phage-encoded cementing proteins. These data indicate a new, OMP-related phage:host linkage, adding to previous knowledge that some lambdoid bacteriophage genomes contain OmpC-like genes that express phage-encoded porins in the lysogenic state.

  11. Convective and large-scale mass flux profiles over tropical oceans determined from synergistic analysis of a suite of satellite observations

    Science.gov (United States)

    Masunaga, Hirohiko; Luo, Zhengzhao Johnny

    2016-07-01

    A new, satellite-based methodology is developed to evaluate convective mass flux and large-scale total mass flux. To derive the convective mass flux, candidate profiles of in-cloud vertical velocity are first constructed with a simple plume model under the constraint of ambient sounding and then narrowed down to the solution that matches satellite-derived cloud top buoyancy. Meanwhile, the large-scale total mass flux is provided separately from satellite soundings by a method developed previously. All satellite snapshots are sorted into a composite time series that delineates the evolution of a vigorous and organized convective system. Principal findings are the following. First, convective mass flux is modulated primarily by convective cloud cover, with the intensity of individual convection being less variable over time. Second, convective mass flux dominates the total mass flux only during the early hours of the convective evolution; as convective system matures, a residual mass flux builds up in the mass flux balance that is reminiscent of stratiform dynamics. The method developed in this study is expected to be of unique utility for future observational diagnosis of tropical convective dynamics and for evaluation of global climate model cumulus parameterizations in a global sense.

  12. Enrofloxacin Permeation Pathways across the Porin OmpC.

    Science.gov (United States)

    Prajapati, Jigneshkumar Dahyabhai; Solano, Carlos José Fernández; Winterhalter, Mathias; Kleinekathöfer, Ulrich

    2018-02-01

    In Gram-negative bacteria, the lack or quenching of antibiotic translocation across the outer membrane is one of the main factors for acquiring antibiotic resistance. An atomic-level comprehension of the key features governing the transport of drugs by outer-membrane protein channels would be very helpful in developing the next generation of antibiotics. In a previous study [ J. D. Prajapati et al. J. Chem. Theory Comput. 2017 , 13 , 4553 ], we characterized the diffusion pathway of a ciprofloxacin molecule through the outer membrane porin OmpC of Escherichia coli by combining metadynamics and a zero-temperature string method. Here, we evaluate the diffusion route through the OmpC porin for a similar fluoroquinolone, that is, the enrofloxacin molecule, using the previously developed protocol. As a result, it was found that the lowest-energy pathway was similar to that for ciprofloxacin; namely, a reorientation was required on the extracellular side with the carboxyl group ahead before enrofloxacin reached the constriction region. In turn, the free-energy basins for both antibiotics are located at similar positions in the space defined by selected reaction coordinates, and their affinity sites share a wide number of porin residues. However, there are some important deviations due to the chemical differences of these two drugs. On the one hand, a slower diffusion process is expected for enrofloxacin, as the permeation pathway exhibits higher overall energy barriers, mainly in the constriction region. On the other hand, enrofloxacin needs to replace some polar interactions in its affinity sites with nonpolar ones. This study demonstrates how minor chemical modifications can qualitatively affect the translocation mechanism of an antibiotic molecule.

  13. RAJA Performance Suite

    Energy Technology Data Exchange (ETDEWEB)

    2017-09-01

    The RAJA Performance Suite is designed to evaluate performance of the RAJA performance portability library on a wide variety of important high performance computing (HPC) algorithmic lulmels. These kernels assess compiler optimizations and various parallel programming model backends accessible through RAJA, such as OpenMP, CUDA, etc. The Initial version of the suite contains 25 computational kernels, each of which appears in 6 variants: Baseline SequcntiaJ, RAJA SequentiaJ, Baseline OpenMP, RAJA OpenMP, Baseline CUDA, RAJA CUDA. All variants of each kernel perform essentially the same mathematical operations and the loop body code for each kernel is identical across all variants. There are a few kernels, such as those that contain reduction operations, that require CUDA-specific coding for their CUDA variants. ActuaJ computer instructions executed and how they run in parallel differs depending on the parallel programming model backend used and which optimizations are perfonned by the compiler used to build the Perfonnance Suite executable. The Suite will be used primarily by RAJA developers to perform regular assessments of RAJA performance across a range of hardware platforms and compilers as RAJA features are being developed. It will also be used by LLNL hardware and software vendor panners for new defining requirements for future computing platform procurements and acceptance testing. In particular, the RAJA Performance Suite will be used for compiler acceptance testing of the upcoming CORAUSierra machine {initial LLNL delivery expected in late-2017/early 2018) and the CORAL-2 procurement. The Suite will aJso be used to generate concise source code reproducers of compiler and runtime issues we uncover so that we may provide them to relevant vendors to be fixed.

  14. Bacteroides Fragilis OmpA: Utility as a Live Vaccine Vector for Biodefense Agents

    Science.gov (United States)

    2009-01-01

    prominent pathogen in adult periodontal diseases 62 (Mineoka et al., 2008). OmpA proteins are among the most conserved of all OMPs in bacteria and consist...only involves growing the modified bacteria . We have met these goals and using recent and novel advances in cloning techniques, designed several...seen a precipitous rise in the percentages of multi- antimicrobial resistant bacteria . Over the past 15 years, scientists have developed experimental

  15. OmpA Binding Mediates the Effect of Antimicrobial Peptide LL-37 on Acinetobacter baumannii.

    Directory of Open Access Journals (Sweden)

    Ming-Feng Lin

    Full Text Available Multidrug-resistant Acinetobacter baumannii has recently emerged as an important pathogen in nosocomial infection; thus, effective antimicrobial regimens are urgently needed. Human antimicrobial peptides (AMPs exhibit multiple functions and antimicrobial activities against bacteria and fungi and are proposed to be potential adjuvant therapeutic agents. This study examined the effect of the human cathelicidin-derived AMP LL-37 on A. baumannii and revealed the underlying mode of action. We found that LL-37 killed A. baumannii efficiently and reduced cell motility and adhesion. The bacteria-killing effect of LL-37 on A. baumannii was more efficient compared to other AMPs, including human ß-defensin 3 (hBD3 and histatin 5 (Hst5. Both flow cytometric analysis and immunofluorescence staining showed that LL-37 bound to A. baumannii cells. Moreover, far-western analysis demonstrated that LL-37 could bind to the A. baumannii OmpA (AbOmpA protein. An ELISA assay indicated that biotin-labelled LL-37 (BA-LL37 bound to the AbOmpA74-84 peptide in a dose-dependent manner. Using BA-LL37 as a probe, the ~38 kDa OmpA signal was detected in the wild type but the ompA deletion strain did not show the protein, thereby validating the interaction. Finally, we found that the ompA deletion mutant was more sensitive to LL-37 and decreased cell adhesion by 32% compared to the wild type. However, ompA deletion mutant showed a greatly reduced adhesion defect after LL-37 treatment compared to the wild strain. Taken together, this study provides evidence that LL-37 affects A. baumannii through OmpA binding.

  16. Antibody Reactivity to Omp31 from Brucella melitensis in Human and Animal Infections by Smooth and Rough Brucellae

    Science.gov (United States)

    Cassataro, Juliana; Pasquevich, Karina; Bruno, Laura; Wallach, Jorge C.; Fossati, Carlos A.; Baldi, Pablo C.

    2004-01-01

    Group 3 of outer membrane proteins (OMPs) of Brucella includes Omp25 and Omp31, which share 34% identity. Omp25 is highly conserved in Brucella species, and Omp31 is present in all Brucella species, except Brucella abortus. Antibodies to Brucella melitensis Omp31 have been sought only in infected sheep, and Western blotting of sera from infected sheep did not reveal anti-Omp31 reactivity. We obtained recombinant purified Omp31 (B. melitensis) and tested its recognition by sera from humans and animals suffering from brucellosis by an indirect enzyme-linked immunosorbent assay (ELISA). Serum samples from 74 patients, 57 sheep, and 47 dogs were analyzed; brucellosis was confirmed by bacteriological isolation in all ovine and canine cases and 31 human cases of brucellosis. Thirty-five patients (47%) were positive for antibodies to Omp31, including seven cases of Brucella suis infection, two cases of B. abortus infection, and three cases of B. melitensis infection. Of 39 sheep naturally infected with B. melitensis (biovars 1 and 3), 23 (59%) were positive for antibodies to Omp31. Anti-Omp31 antibodies were also detected in 12 of 18 rams (67%) in which Brucella ovis was isolated from semen. Antibodies to Omp31 were also found in 41 (87%) of the 47 dogs, including 13 with recent infection. These results suggest that an indirect ELISA using recombinant purified Omp31 from B. melitensis would be of limited value for the diagnosis of human and animal brucellosis. Nevertheless, the potential usefulness of this antigen in combination with other recombinant proteins from Brucella should not be dismissed.   PMID:14715555

  17. Associations of Escherichia coli K-12 OmpF trimers with rough and smooth lipopolysaccharides

    International Nuclear Information System (INIS)

    Diedrich, D.L.; Stein, M.A.; Schnaitman, C.A.

    1990-01-01

    The associations of both rough and smooth lipopolysaccharides (LPS) with the OmpF porin of Escherichia coli K-12 were examined in galE strains deleted for ompC. Transformation with pSS37 and growth with galactose conferred the ability to assemble a Shigella dysenteriae O antigen onto the core oligosaccharide of E. coli K-12 LPS. The association of LPS with OmpF trimers was assessed by staining, autoradiography of LPS specifically labeled with [1-14C]galactose, and Western immunoblotting with a monoclonal antibody specific for OmpF trimers. These techniques revealed that the migration distances and multiple banding patterns of OmpF porin trimers in sodium dodecyl sulfate-polyacrylamide gels were dictated by the chemotype of associated LPS. Expression of smooth LPS caused almost all of the trimeric OmpF to run in gels with a slower mobility than trimers from rough strains. The LPS associated with trimers from a smooth strain differed from the bulk-phase LPS by consisting almost exclusively of molecules with O antigen

  18. Immune enhancement of Taishan Robinia pseudoacacia polysaccharide on recombinant Proteus mirabilis OmpA in chickens.

    Science.gov (United States)

    Zhang, Yongbing; Yang, Shifa; Zhao, Xue; Yang, Ya; Li, Bing; Zhu, Fujie; Zhu, Ruiliang

    2014-09-01

    This study was conducted to evaluate the effects of Taishan Robinia pseudoacacia polysaccharide (TRPPS) on immune responses of chickens immunized with Proteus mirabilis outer membrane protein A (OmpA) recombinant protein vaccine. OmpA was expressed in Pichia pastoris and mixed with TRPPS. 360 chickens were randomly divided into six groups. Groups I to IV were treated with OmpA which contained TRPPS of three different dosages, Freund's adjuvant, respectively. Groups V and VI were treated with pure OmpA and physiological saline, respectively. The data showed that the antibody titers against OmpA, the concentration of IL-2, CD4 +, and CD8 +, T lymphocyte proliferation rate in Group II were significantly higher (P < 0.05) than those in the other groups, little difference in SIgA content was observed among groups I to VI. These results indicated that TRPPS strengthened humoral and cellular immune responses against recombinant OmpA vaccine. Moreover, 200 mg/mL TRPPS showed significance (P < 0.05) compared with Freund's adjuvant. Therefore, TRPPS can be developed into an adjuvant for recombinant subunit vaccine. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. Brucella ovis PA mutants for outer membrane proteins Omp10, Omp19, SP41, and BepC are not altered in their virulence and outer membrane properties.

    Science.gov (United States)

    Sidhu-Muñoz, Rebeca S; Sancho, Pilar; Vizcaíno, Nieves

    2016-04-15

    Mutants in several genes have been obtained on the genetic background of virulent rough (lacking O-polysaccharide) Brucella ovis PA. The target genes encode outer membrane proteins previously associated with the virulence of smooth (bearing O-polysaccharide chains in the lipopolysaccharide) Brucella strains. Multiple attempts to delete omp16, coding for a homologue to peptidoglycan-associated lipoproteins, were unsuccessful, which suggests that Omp16 is probably essential for in vitro survival of B. ovis PA. Single deletion of omp10 or omp19-that encode two other outer membrane lipoproteins--was achieved, but the simultaneous removal of both genes failed, suggesting an essential complementary function between both proteins. Two other deletion mutants, defective in the Tol-C-homologue BepC or in the SP41 adhesin, were also obtained. Surprisingly when compared to previous results obtained with smooth Brucella, none of the B. ovis mutants showed attenuation in the virulence, either in the mouse model or in cellular models of professional and non-professional phagocytes. Additionally, and in contrast to the observations reported with smooth Brucella strains, several properties related to the outer membrane remained almost unaltered. These results evidence new distinctive traits between naturally rough B. ovis and smooth brucellae. Copyright © 2016 Elsevier B.V. All rights reserved.

  20. Learning DHTMLX suite UI

    CERN Document Server

    Geske, Eli

    2013-01-01

    A fast-paced, example-based guide to learning DHTMLX.""Learning DHTMLX Suite UI"" is for web designers who have a basic knowledge of JavaScript and who are looking for powerful tools that will give them an extra edge in their own application development. This book is also useful for experienced developers who wish to get started with DHTMLX without going through the trouble of learning its quirks through trial and error. Readers are expected to have some knowledge of JavaScript, HTML, Document Object Model, and the ability to install a local web server.

  1. OmpL1 is an extracellular matrix- and plasminogen-interacting protein of Leptospira spp.

    Science.gov (United States)

    Fernandes, Luis G V; Vieira, Monica L; Kirchgatter, Karin; Alves, Ivy J; de Morais, Zenaide M; Vasconcellos, Silvio A; Romero, Eliete C; Nascimento, Ana L T O

    2012-10-01

    Leptospirosis is a zoonosis with multisystem involvement caused by pathogenic strains of the genus Leptospira. OmpL1 is an outer membrane protein of Leptospira spp. that is expressed during infection. In this work, we investigated novel features of this protein. We describe that OmpL1 is a novel leptospiral extracellular matrix (ECM)-binding protein and a plasminogen (PLG) receptor. The recombinant protein was expressed in Escherichia coli BL21(DE3) Star/pLysS as inclusion bodies, refolded, and purified by metal-chelating chromatography. The protein presented a typical β-strand secondary structure, as evaluated by circular dichroism spectroscopy. The recombinant protein reacted with antibodies in serum samples from convalescent leptospirosis patients with a high specificity compared to serum samples from individuals with unrelated diseases. These data strengthen the usefulness of OmpL1 as a diagnostic marker of leptospirosis. The characterization of the immunogenicity of recombinant OmpL1 in inoculated BALB/c mice showed that the protein has the capacity to elicit humoral and cellular immune responses, as denoted by high antibody titers and the proliferation of lymphocytes. We demonstrate that OmpL1 has the ability to mediate attachment to laminin and plasma fibronectin, with K(D) (equilibrium dissociation constant) values of 2,099.93 ± 871.03 nM and 1,239.23 ± 506.85 nM, respectively. OmpL1 is also a PLG receptor, with a K(D) of 368.63 ± 121.23 nM, capable of generating enzymatically active plasmin. This is the first report that shows and characterizes OmpL1 as an ECM-interacting and a PLG-binding protein of Leptospira spp. that may play a role in bacterial pathogenesis when expressed during infection.

  2. Effects of beta-lactamases and omp mutation on susceptibility to beta-lactam antibiotics in Escherichia coli.

    Science.gov (United States)

    Hiraoka, M; Okamoto, R; Inoue, M; Mitsuhashi, S

    1989-01-01

    Four types of beta-lactamases consisting of a penicillinase type I (TEM-1), a penicillinase type II (OXA-1), a cephalosporinase of Citrobacter freundii, and a cephalosporinase of Proteus vulgaris were introduced into Escherichia coli MC4100 and its omp mutants, MH1160 (MC4100 ompR1) and MH760 (MC4100 ompR2), by transformation. Effects of the combination of the omp mutations and these beta-lactamases on the susceptibility of E. coli strains were studied with 15 beta-lactam antibiotics including cephalosporins, cephamycins, penicillins, imipenem, and aztreonam. The ompR1 mutant, MH1160, lacks OmpF and OmpC, and it showed reduced susceptibility to 11 of the 15 beta-lactam agents. The reduction in susceptibility to cefoxitin, moxalactam, and flomoxef was much greater than reduction in susceptibility to the other agents. When the ompR1 mutant produced the cephalosporinase of C. freundii, the susceptibility of the mutant to 12 of the 15 beta-lactam antibiotics decreased. The reduction in susceptibility of MH1160 to 10 of the 12 agents affected by the enzyme was two- to fourfold greater than that observed in MC4100. Such a synergistic effect was also observed with the cephalosporinase of P. vulgaris and ompR1 mutation against six cephalosporins, moxalactam, and aztreonam. Images PMID:2658786

  3. Continuation of Global NO2 and SO2 Monitoring with Suomi NPP OMPS

    Science.gov (United States)

    Yang, K.; Zhang, H.; Wang, J.; Ge, C.; Wang, Y.

    2017-12-01

    We have produced high-quality NO2 and SO2 standard products (named NMNO2 and NMSO2 respectively) from the SNPP OMPS-NM daily global observations. These OMPS standard products have been archived and publicly released at NASA Goddard Earth Sciences Data and Information Services Center (https://daac.gsfc.nasa.gov/information/news/595e9675624016d1af392c73/omps-nm-no- 2-and-so-2-l-2-data-products-released). Analyses and comparisons have demonstrated that the qualities of these OMPS standard products match or surpass those of the corresponding OMI products, enabling the continuity and extension of these two key standard Earth System Data Records (ESDRs) that begun with NASA's EOS Aura mission using the SNPP observations. In this presentation, we summarize the new techniques and algorithm advances that improve the accuracy and consistency of these ESDRs from satellite observations, and highlight the regional changes in NO2 and SO2 detected from half a decade of SNPP OMPS observations.

  4. Clementine sensor suite

    Energy Technology Data Exchange (ETDEWEB)

    Ledebuhr, A.G. [Lawrence Livermore National Lab., CA (United States)

    1994-11-15

    LLNL designed and built the suite of six miniaturized light-weight space-qualified sensors utilized in the Clementine mission. A major goal of the Clementine program was to demonstrate technologies originally developed for Ballistic Missile Defense Organization Programs. These sensors were modified to gather data from the moon. This overview presents each of these sensors and some preliminary on-orbit performance estimates. The basic subsystems of these sensors include optical baffles to reject off-axis stray light, light-weight ruggedized optical systems, filter wheel assemblies, radiation tolerant focal plane arrays, radiation hardened control and readout electronics and low mass and power mechanical cryogenic coolers for the infrared sensors. Descriptions of each sensor type are given along with design specifications, photographs and on-orbit data collected.

  5. Immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody.

    Science.gov (United States)

    Zheng, W Y; Wang, Y; Zhang, Z C; Yan, F

    2015-10-05

    We examined the immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody. Genomic DNA from the M5 strain of goat Brucella was amplified by polymerase chain reaction and cloned into the prokaryotic expression vector pGEX-4T-1. The expression and immunological characteristics of the fusion protein GST-omp31 were subjected to preliminary western blot detection with goat Brucella rabbit immune serum. The Brucella immunized BALB/c mouse serum was detected using purified protein. The high-potency mouse splenocytes and myeloma Sp2/0 cells were fused. Positive clones were screened by enzyme-linked immunosorbent assay to establish a hybridoma cell line. Mice were inoculated intraperitoneally with hybridoma cells to prepare ascites. The mAb was purified using the n-caprylic acid-ammonium sulfate method. The characteristics of mAb were examined using western blotting and enzyme-linked immunosorbent assay. A 680-base pair band was observed after polymerase chain reaction. Enzyme digestion identification and sequencing showed that the pGEX-4T-1-omp31 prokaryotic expression vector was successfully established; a target band of approximately 57 kDa with an apparent molecular weight consistent with the size of the target fusion protein. At 25°C, the expression of soluble expression increased significantly; the fusion protein GST-omp31 was detected by western blotting. Anti-omp31 protein mAb was obtained from 2 strains of Brucella. The antibody showed strong specificity and sensitivity and did not cross-react with Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Mycobacterium tuberculosis, or Bacillus pyocyaneus. The pGEX-4T-1-omp31 prokaryotic expression vector was successfully established and showed good immunogenicity. The antibody also showed strong specificity and good sensitivity.

  6. Modulation of inv gene expression by the OmpR two-component response regulator protein of Yersinia enterocolitica.

    Science.gov (United States)

    Raczkowska, A; Brzóstkowska, M; Kwiatek, A; Bielecki, J; Brzostek, K

    2011-07-01

    To elucidate the physiological meaning of OmpR-dependent expression of invasin gene (inv) inhibition in Yersinia enterocolitica, the function of the EnvZ/OmpR regulatory pathway in osmoregulation of inv expression was analyzed in detail. The osmoregulation of inv expression was found to be a multifaceted process involving both OmpR-dependent and -independent mechanisms. Analysis of inv transcription in strains lacking OmpR or EnvZ proteins indicated that kinase EnvZ is not the only regulator of OmpR phosphorylation. Using the transcriptional inv::lacZ fusion in a heterologous system (Escherichia coli) we tried to clarify the role of OmpR in the inv regulatory circuit composed of negative (H-NS) and positive (RovA) regulators of inv gene transcription. We were able to show a significant increase in inv expression in E. coli ompR background under H-NS( Ecoli )-repressed condition. Moreover, H-NS-mediated inv repression was relieved when RovA of Y. enterocolitica was expressed from a plasmid. Furthermore, we showed that RovA may activate inv expression irrespective on the presence of H-NS protein. Using this strategy we showed that OmpR of Y. enterocolitica decrease RovA-mediated inv activation.

  7. Protective immunity induced by the vaccination of recombinant Proteus mirabilis OmpA expressed in Pichia pastoris.

    Science.gov (United States)

    Zhang, Yongbing; Yang, Shifa; Dai, Xiumei; Liu, Liping; Jiang, Xiaodong; Shao, Mingxu; Chi, Shanshan; Wang, Chuanwen; Yu, Cuilian; Wei, Kai; Zhu, Ruiliang

    2015-01-01

    Proteus mirabilis (P. mirabilis) is a zoonotic pathogen that has recently presented a rising infection rate in the poultry industry. To develop an effective vaccine to protect chickens against P. mirabilis infection, OmpA, one of the major outer membrane proteins of P. mirabilis, was expressed in Pichia pastoris. The concentration of the expressed recombinant OmpA protein reached 8.0μg/mL after induction for 96h with 1.0% methanol in the culture. In addition, OmpA protein was confirmed by SDS-PAGE and Western blot analysis using the antibody against Escherichia coli-expressed OmpA protein. Taishan Pinus massoniana pollen polysaccharide, a known plant-derived adjuvant, was mixed into the recombinant OmpA protein to prepare the OmpA subunit vaccine. We then subcutaneously inoculated this vaccine into chickens to examine the immunoprotective effects. ELISA analysis indicated that an excellent antibody response against OmpA was elicited in the vaccinated chickens. Moreover, a high protection rate of 80.0% was observed in the vaccinated group, which was subsequently challenged with P. mirabilis. The results suggest that the eukaryotic P. mirabilis OmpA was an ideal candidate protein for developing an effective subunit vaccine against P. mirabilis infection. Copyright © 2014 Elsevier Inc. All rights reserved.

  8. Energy Efficiency Experiments on Samsung Exynos 5 Heterogeneous Multicore using OmpSs Task Based Programming

    OpenAIRE

    Holmgren, Rune

    2015-01-01

    This thesis explore the energy efficiency of task based programming with OpenMP SuperScalar (OmpSs) on the heterogeneous Samsung Exynos 5422 system on a chip. The system features small energy efficient cores, large high performance cores and a GPGPU, and OmpSs tasks were run on all three different processors. Experiments running a genetic algorithm and a Cholesky decomposition were used to gather results. The option of running applications on the energy efficient cores, on the high perfo...

  9. Parenteral immunization of PLA/PLGA nanoparticle encapsulating outer membrane protein (Omp) from Aeromonas hydrophila: Evaluation of immunostimulatory action in Labeo rohita (rohu).

    Science.gov (United States)

    Rauta, Pradipta Ranjan; Nayak, Bismita

    2015-05-01

    Advanced vaccine research approaches needs to explore on biodegradable nanoparticles (NPs) based vaccine carrier that can serve as antigen delivery systems as well as immuno-stimulatory action to induce both innate and adaptive immune response in fish. Immunogenicity of PLA and PLGA NPs encapsulating outer membrane protein (Omp) antigen of Aeromonas hydrophila were evaluated through intra-peritoneal injection in fish, Labeo rohita. Antigen loaded PLA-Omp (223.5 ± 13.19 nm) and PLGA-Omp (166.4 ± 21.23 nm) NPs were prepared using double emulsion method by efficiently encapsulating the antigen reaching the encapsulation efficiency 44 ± 4.58% and 59.33 ± 5.13% respectively. Our formulated PLA Omp and PLGA-Omp NPs were in nanometer range (PLA-Omp, it showed considerably slower antigen release in vitro than PLGA-Omp NPs. Other physical properties like zetapotential values and poly dispersity index (PDI) confirmed the stability as well as monodisperse nature of the formulated nanoparticles. The spherical and isolated nature of PLA-Omp and PLGA-Omp NPs were revealed by SEM analysis. Upon immunization of all antigenic formulations (PLA-Omp NP, PLGA-Omp NP, FIA-Omp, PLA NP, PLGA NP, PBS as control), significant higher bacterial agglutination titre and haemolytic activity were observed in case of PLA-Omp and PLGA-Omp immunized groups than rest groups at both 21 days and 42 days. The specific antibody response was significantly increased and persisted up to 42 days of post immunization by PLA-Omp, PLGA-Omp, FIA-Omp. PLA-Omp NPs showed better immune response (higher bacterial agglutination titre, haemolytic activity, specific antibody titre, higher percent survival upon A. hydrophila challenge) than PLGA-Omp in L. rohita confirming its better efficacy. Comparable antibody response of PLA-Omp and PLGA-Omp with FIA-Omp treated groups suggested that PLA and PLGA could be replacement for Freund's adjuvant (for stimulating antibody response) to overcome many side effects

  10. The Outer Membrane Protein OmpW Forms an Eight-Stranded beta-Barrel with a Hydrophobic Channel

    International Nuclear Information System (INIS)

    Hong, H.; Patel, D.; Tamm, L.; van den Berg, B.

    2006-01-01

    Escherichia coli OmpW belongs to a family of small outer membrane (OM) proteins that are widespread in Gram-negative bacteria. Their functions are unknown, but recent data suggest that they may be involved in the protection of bacteria against various forms of environmental stress. In order to gain insight into the function of these proteins we have determined the crystal structure of Escherichia coli OmpW to 2.7 Angstroms resolution. The structure shows that OmpW forms an eight-stranded beta-barrel with a long and narrow hydrophobic channel that contains a bound LDAO detergent molecule. Single channel conductance experiments show that OmpW functions as an ion channel in planar lipid bilayers. The channel activity can be blocked by the addition of LDAO. Taken together, the data suggest that members of the OmpW family could be involved in the transport of small hydrophobic molecules across the bacterial OM

  11. Organic micropollutants (OMPs) in natural waters: Oxidation by UV/H2O2 treatment and toxicity assessment.

    Science.gov (United States)

    Rozas, Oscar; Vidal, Cristiane; Baeza, Carolina; Jardim, Wilson F; Rossner, Alfred; Mansilla, Héctor D

    2016-07-01

    Organic micropollutants (OMPs) are ubiquitous in natural waters even in places where the human activity is limited. The presence of OMPs in natural water sources for human consumption encourages the evaluation of different water purification technologies to ensure water quality. In this study, the Biobío river (Chile) was selected since the watershed includes urban settlements and economic activities (i.e. agriculture, forestry) that incorporate a variety of OMPs into the aquatic environment, such as pesticides, pharmaceuticals and personal care products. Atrazine (herbicide), caffeine (psychotropic), diclofenac (anti-inflammatory) and triclosan (antimicrobial) in Biobío river water and in different stages of a drinking and two wastewater treatment plants downstream Biobío river were determined using solid phase extraction (SPE) and liquid chromatography/tandem mass spectrometry (LC-MS/MS) and electrospray ionization (ESI). Quantification of these four compounds showed concentrations in the range of 8 ± 2 to 55 ± 10 ng L(-1) in Biobío river water, 11 ± 2 to 74 ± 21 ng L(-1) in the drinking water treatment plant, and 60 ± 10 to 15,000 ± 1300 ng L(-1) in the wastewater treatment plants. Caffeine was used as an indicator of wastewater discharges. Because conventional water treatment technologies are not designed to eliminate some emerging organic pollutants, alternative treatment processes, UV and UV/H2O2, were employed. The transformation of atrazine, carbamazepine (antiepileptic), diclofenac and triclosan was investigated at laboratory scale. Both processes were tested at different UV doses and the Biobío river water matrix effects were evaluated. Initial H2O2 concentration used was 10 mg L(-1). Results showed that, the transformation profile obtained using UV/H2O2 at UV doses up to 900 mJ cm(-2), followed the trend of diclofenac > triclosan > atrazine > carbamazepine. Furthermore acute toxicity tests with Daphnia magna were carried

  12. Revealing genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles under osmotic stress in Escherichia coli K-12 MG1655

    DEFF Research Database (Denmark)

    Seo, Sang Woo; Gao, Ye; Kim, Donghyuk

    2017-01-01

    ) belong to OmpR regulon. Among them, 26 genes show more than two-fold changes in expression level in an OmpR knock-out strain. Specifically, we find that: 1) OmpR regulates mostly membrane-located gene products involved in diverse fundamental biological processes, such as narU (encoding nitrate/nitrite...

  13. Profiles

    International Nuclear Information System (INIS)

    2004-01-01

    Profiles is a synthetic overview of more than 100 national energy markets in the world, providing insightful facts and key energy statistics. A Profile is structured around 6 main items and completed by key statistics: Ministries, public agencies, energy policy are concerned; main companies in the oil, gas, electricity and coal sectors, status, shareholders; reserve, production, imports and exports, electricity and refining capacities; deregulation of prices, subsidies, taxes; consumption trends by sector, energy market shares; main energy projects, production and consumption prospects. Statistical Profiles are present in about 3 pages the main data and indicators on oil, gas, coal and electricity. (A.L.B.)

  14. Apparatus for storing protective suits

    International Nuclear Information System (INIS)

    Englemann, H.J.; Koller, J.; Schrader, H.R.; Schade, G.; Pedrerol, J.

    1975-01-01

    Arrangements are described for storing one or more protective suits when contaminated on the outside. In order to permit a person wearing a contaminated suit to leave a contaminated area safely, and without contaminating the environment, it has hitherto been the practice for the suit to be passed through a 'lock' and cleansed under decontaminating showers whilst still being worn. This procedure is time wasting and not always completely effective, and it may be necessary to provide a second suit for use whilst the first suit is being decontaminated. Repeated decontamination may also result in undue wear and tear. The arrangements described provide a 'lock' chamber in which a contaminated suit may be stowed away without its interior becoming contaminated, thus allowing repeated use by persons donning and shedding it. (U.K.)

  15. An integrative in silico approach to the structure of Omp33-36 in Acinetobacter baumannii.

    Science.gov (United States)

    Jahangiri, Abolfazl; Rasooli, Iraj; Owlia, Parviz; Fooladi, Abbas Ali Imani; Salimian, Jafar

    2018-02-01

    Omp33-36 in A. baumannii, a bacterium causing serious nosocomial infections, is a virulence factor associated with the pathogen metabolic fitness as well as its adherence and invasion to human epithelial cells. This protein is also involved in interaction of the bacteria with host cells by binding to fibronectin. Moreover, Omp33-36 renders cytotoxicity to A. baumannii in addition to inducing apoptosis and modulation of autophagy. In the present study, an integrated strategy is launched to pierce into the 3D structure of Omp33-36 protein. The signal peptide within the sequence was determined, then, topology as well as secondary and tertiary structures of the protein were predicted. The mature protein assigned as a 14-stranded barrel in which residues 1-19 is removed as signal peptide. The obtained 3D models were evaluated in terms of quality; and then, served as queries to find similar protein structures. The hits were analyzed regarding topology among which 14-stranded were considered. The most qualified model was refined and then its sequence aligned to its counterpart similar structure protein (CymA from Klebsiella oxytoca). The determined structure of Omp33-36 could justify its porin function and carbapenem-resistance associated with the loss of this protein. Copyright © 2018. Published by Elsevier Ltd.

  16. Structure of Salmonella typhimurium OMP Synthase in a Complete Substrate Complex

    DEFF Research Database (Denmark)

    Grubmeyer, Charles; Hansen, Michael Riis; Fedorov, Alexander A.

    2012-01-01

    Dimeric Salmonella typhimurium orotate phosphoribosyltransferase (OMP synthase, EC 2.4.2.10), a key enzyme in de novo pyrimidine nucleotide synthesis, has been cocrystallized in a complete substrate E·MgPRPP·orotate complex and the structure determined to 2.2 Å resolution. This structure resem...

  17. Structures of the OmpF porin crystallized in the presence of foscholine-12.

    Science.gov (United States)

    Kefala, Georgia; Ahn, Chihoon; Krupa, Martin; Esquivies, Luis; Maslennikov, Innokentiy; Kwiatkowski, Witek; Choe, Senyon

    2010-05-01

    The endogenous Escherichia coli porin OmpF was crystallized as an accidental by-product of our efforts to express, purify, and crystallize the E. coli integral membrane protein KdpD in the presence of foscholine-12 (FC12). FC12 is widely used in membrane protein studies, but no crystal structure of a protein that was both purified and crystallized with this detergent has been reported in the Protein Data Bank. Crystallization screening for KdpD yielded two different crystals of contaminating protein OmpF. Here, we report two OmpF structures, the first membrane protein crystal structures for which extraction, purification, and crystallization were done exclusively with FC12. The first structure was refined in space group P21 with cell parameters a = 136.7 A, b = 210.5 A, c = 137 A, and beta = 100.5 degrees , and the resolution of 3.8 A. The second structure was solved at the resolution of 4.4 A and was refined in the P321 space group, with unit cell parameters a = 215.5 A, b = 215.5 A, c = 137.5 A, and gamma = 120 degrees . Both crystal forms show novel crystal packing, in which the building block is a tetrahedral arrangement of four trimers. Additionally, we discuss the use of FC12 for membrane protein crystallization and structure determination, as well as the problem of the OmpF contamination for membrane proteins overexpressed in E. coli.

  18. Comparison of ompP5 sequence-based typing and pulsed-filed gel ...

    African Journals Online (AJOL)

    Yomi

    2012-03-15

    Mar 15, 2012 ... 37°C in a 5% CO2-enriched atmosphere. Polymerase chain .... A 25. B5. HM747099. Zhang et al., 2011. SC099 (serovar15). Brain. 26. A 26. B6 .... was more effective than ompP5-based typing method at genotyping H.

  19. [Different aluminum adjuvants significantly enhances the effect of immunization on Brucella Omp31].

    Science.gov (United States)

    Qing, Rui; Xiang, Qingke; Liu, Zhongqi; Xiao, Fei; Yang, Fan

    2018-02-01

    Objective To investigate the effect of aluminum phosphate (AP) and aluminum hydroxide (AH) as adjuvants on Brucella outer membrane protein 31 (Omp31) in inducing humoral and cellular immune responses and immune protection. Methods AP and AH adjuvants were prepared and separately mixed with Brucella Omp31 protein to measure the adsorption rates. The AP- and AH-absorbed Omp31 protein were intraperitoneally injected into BLAB/c mice at 0, 2, and 4 weeks, and meanwhile, unabsorbed Omp31 protein and PBS were used as controls. The levels of serum IgG, IgG1, IgG2a and genital tract secretion sIgA were determined by ELISA at 0, 2, 4 and 6 weeks. Spleen cells were collected for culture at 6 weeks, and the cells were stimulated by Omp31 for 48 hours followed by the analysis of IFN-γ and IL-10 levels in the supernatants by ELISA, and the determination of lymphocyte proliferation by CCK-8 assay. The mice were challenged with Brucella at 6 weeks, and bacterial content in spleen tissue was determined 1 and 2 weeks later. Results AP and AH could absorb over 70% and 85% of the Omp31 protein, respectively, for solutions at all the tested concentrations. ELISA suggested that serum IgG, IgG1, IgG2a and genital tract sIgA levels peaked 2 weeks after the last immunization for both AP and AH groups, and antibody level was higher in the AP and AH groups than the control groups, and higher in the AH group than in the AP group. CCK-8 assay showed that the proliferating rate of lymphocytes induced by the AH group was significantly higher than that by the AP group, and the AH group also showed significantly higher IFN-γ level in the supernatant than the AP group, but no significant difference in IL-10 level. The AH group had remarkably lower bacterial load in the spleen than the AP group 2 weeks after challenged by Brucella 16M strain. Conclusion Both AP and AH adjuvants effectively enhanced immunogenicity and immune protection of the Brucella Omp31 protein, and AH was superior to AP in

  20. An open labeled, comparative clinical study on efficacy and tolerability of oral minipulse of steroid (OMP alone, OMP with PUVA and broad / narrow band UVB phototherapy in progressive vitiligo

    Directory of Open Access Journals (Sweden)

    Rath Namita

    2008-01-01

    Full Text Available Background: Several modalities of treatment have been tried in vitiligo with varied results; however, Indian data on comparative studies of two or more therapies are limited. Aims: We compared different phototherapy methods with an oral steroid as an adjunct to determine the method with the best tolerability and efficacy. Methods: Eighty-six patients with progressive vitiligo were randomly assigned to different study groups according to a continuous selection method over a period of one year. Group 1 was given OMP + PUVA, group 2 OMP + UVB (NB, group 3 OMP + UVB (BB and group 4 was given OMP alone. Each patient was followed up for six months and then released from treatment. Clinical evaluation was made at the end of three and six months. Results: In group 1 (OMP + PUVA, marked improvement was seen in 18.51% while moderate improvement was seen in 66.66% of the patients. Marked improvement was seen in 37.03% in group 2 (OMP + NB-UVB while 44.44% had moderate improvement. In group 3 (OMP + BB UVB, 8.33% showed marked improvement while moderate improvement was seen in 25% of the patients. Marked and moderate improvement was seen in 5 and 10% of group 4 (OMP patients, respectively. Conclusions: Our study compared four treatment modalities in vitiligo patients, out of which oral minipulse of steroids (OMP only had an adjunct value and was not very effective by itself. Narrow band UVB has a definite edge over broad band UVB and should be preferred when both options are available. NB-UVB and PUVA showed comparable efficacy.

  1. Homology analysis and cross-immunogenicity of OmpA from pathogenic Yersinia enterocolitica, Yersinia pseudotuberculosis and Yersinia pestis.

    Science.gov (United States)

    Chen, Yuhuang; Duan, Ran; Li, Xu; Li, Kewei; Liang, Junrong; Liu, Chang; Qiu, Haiyan; Xiao, Yuchun; Jing, Huaiqi; Wang, Xin

    2015-12-01

    The outer membrane protein A (OmpA) is one of the intra-species conserved proteins with immunogenicity widely found in the family of Enterobacteriaceae. Here we first confirmed OmpA is conserved in the three pathogenic Yersinia: Yersinia pestis, Yersinia pseudotuberculosis and pathogenic Yersinia enterocolitica, with high homology at the nucleotide level and at the amino acid sequence level. The identity of ompA sequences for 262 Y. pestis strains, 134 Y. pseudotuberculosis strains and 219 pathogenic Y. enterocolitica strains are 100%, 98.8% and 97.7% similar. The main pattern of OmpA of pathogenic Yersinia are 86.2% and 88.8% identical at the nucleotide and amino acid sequence levels, respectively. Immunological analysis showed the immunogenicity of each OmpA and cross-immunogenicity of OmpA for pathogenic Yersinia where OmpA may be a vaccine candidate for Y. pestis and other pathogenic Yersinia. Copyright © 2015 Elsevier Ltd. All rights reserved.

  2. Adobe Creative Suite 4 Bible

    CERN Document Server

    Padova, Ted

    2009-01-01

    As one of the few books to cover integration and workflow issues between Photoshop, Illustrator, InDesign, GoLive, Acrobat, and Version Cue, this comprehensive reference is the one book that Creative Suite users need; Two well-known and respected authors cover topics such as developing consistent color-managed workflows, moving files among the Creative Suite applications, preparing files for print or the Web, repurposing documents, and using the Creative Suite with Microsoft Office documents; More than 1,200 pages are packed with valuable advice and techniques for tackling common everyday issu

  3. EDL Sensor Suite, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Optical Air Data Systems (OADS) L.L.C. proposes a LIDAR based remote measurement sensor suite capable of satisfying a significant number of the desired sensing...

  4. Satellite Ocean Heat Content Suite

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This collection contains an operational Satellite Ocean Heat Content Suite (SOHCS) product generated by NOAA National Environmental Satellite, Data, and Information...

  5. EVA Suit Microbial Leakage Investigation

    Data.gov (United States)

    National Aeronautics and Space Administration — The objective of this project is to collect microbial samples from various EVA suits to determine how much microbial contamination is typically released during...

  6. OMPS/NPP PCA SO2 Total Column 1-Orbit L2 Swath 50x50km NRT

    Data.gov (United States)

    National Aeronautics and Space Administration — The OMPS-NPP L2 NM Sulfur Dioxide (SO2) Total and Tropospheric Column swath orbital collection 2 version 2.0 product contains the retrieved sulfur dioxide (SO2)...

  7. Space Suit Joint Torque Testing

    Science.gov (United States)

    Valish, Dana J.

    2011-01-01

    In 2009 and early 2010, a test was performed to quantify the torque required to manipulate joints in several existing operational and prototype space suits in an effort to develop joint torque requirements appropriate for a new Constellation Program space suit system. The same test method was levied on the Constellation space suit contractors to verify that their suit design meets the requirements. However, because the original test was set up and conducted by a single test operator there was some question as to whether this method was repeatable enough to be considered a standard verification method for Constellation or other future space suits. In order to validate the method itself, a representative subset of the previous test was repeated, using the same information that would be available to space suit contractors, but set up and conducted by someone not familiar with the previous test. The resultant data was compared using graphical and statistical analysis and a variance in torque values for some of the tested joints was apparent. Potential variables that could have affected the data were identified and re-testing was conducted in an attempt to eliminate these variables. The results of the retest will be used to determine if further testing and modification is necessary before the method can be validated.

  8. Regulatory protein OmpR influences the serum resistance of Yersinia enterocolitica O:9 by modifying the structure of the outer membrane.

    Directory of Open Access Journals (Sweden)

    Karolina Skorek

    Full Text Available The EnvZ/OmpR two-component system constitutes a regulatory pathway involved in bacterial adaptive responses to environmental cues. Our previous findings indicated that the OmpR regulator in Yersinia enterocolitica O:9 positively regulates the expression of FlhDC, the master flagellar activator, which influences adhesion/invasion properties and biofilm formation. Here we show that a strain lacking OmpR grown at 37°C exhibits extremely high resistance to the bactericidal activity of normal human serum (NHS compared with the wild-type strain. Analysis of OMP expression in the ompR mutant revealed that OmpR reciprocally regulates Ail and OmpX, two homologous OMPs of Y. enterocolitica, without causing significant changes in the level of YadA, the major serum resistance factor. Analysis of mutants in individual genes belonging to the OmpR regulon (ail, ompX, ompC and flhDC and strains lacking plasmid pYV, expressing YadA, demonstrated the contribution of the respective proteins to serum resistance. We show that Ail and OmpC act in an opposite way to the OmpX protein to confer serum resistance to the wild-type strain, but are not responsible for the high resistance of the ompR mutant. The serum resistance phenotype of ompR seems to be multifactorial and mainly attributable to alterations that potentiate the function of YadA. Our results indicate that a decreased level of FlhDC in the ompR mutant cells is partly responsible for the serum resistance and this effect can be suppressed by overexpression of flhDC in trans. The observation that the loss of FlhDC enhances the survival of wild-type cells in NHS supports the involvement of FlhDC regulator in this phenotype. In addition, the ompR mutant exhibited a lower level of LPS, but this was not correlated with changes in the level of FlhDC. We propose that OmpR might alter the susceptibility of Y. enterocolitica O:9 to complement-mediated killing through remodeling of the outer membrane.

  9. ompW is cooperatively upregulated by MarA and SoxS in response to menadione.

    Science.gov (United States)

    Collao, B; Morales, E H; Gil, F; Calderón, I L; Saavedra, C P

    2013-04-01

    OmpW is a minor porin whose biological function has not been clearly defined. Evidence obtained in our laboratory indicates that in Salmonella enterica serovar Typhimurium the expression of OmpW is activated by SoxS upon exposure to paraquat and it is required for resistance. SoxS belongs to the AraC family of transcriptional regulators, like MarA and Rob. Due to their high structural similarity, the genes under their control have been grouped in the mar/sox/rob regulon, which presents a DNA-binding consensus sequence denominated the marsox box. In this work, we evaluated the role of the transcription factors MarA, SoxS and Rob of S. enterica serovar Typhimurium in regulating ompW expression in response to menadione. We determined the transcript and protein levels of OmpW in different genetic backgrounds; in the wild-type and Δrob strains ompW was upregulated in response to menadione, while in the ΔmarA and ΔsoxS strains the induction was abolished. In a double marA soxS mutant, ompW transcript levels were lowered after exposure to menadione, and only complementation in trans with both genes restored the positive regulation. Using transcriptional fusions and electrophoretic mobility shift assays with mutant versions of the promoter region we demonstrated that two of the predicted sites were functional. Additionally, we demonstrated that MarA increases the affinity of SoxS for the ompW promoter region. In conclusion, our study shows that ompW is upregulated in response to menadione in a cooperative manner by MarA and SoxS through a direct interaction with the promoter region.

  10. A Test Suite for Safety-Critical Java using JML

    DEFF Research Database (Denmark)

    Ravn, Anders Peter; Søndergaard, Hans

    2013-01-01

    Development techniques are presented for a test suite for the draft specification of the Java profile for Safety-Critical Systems. Distinguishing features are: specification of conformance constraints in the Java Modeling Language, encoding of infrastructure concepts without implementation bias......, and corresponding specifications of implicitly stated behavioral and real-time properties. The test programs are auto-generated from the specification, while concrete values for test parameters are selected manually. The suite is open source and publicly accessible....

  11. Overexpression of MicA induces production of OmpC-enriched outer membrane vesicles that protect against Salmonella challenge.

    Science.gov (United States)

    Choi, Hyun-Il; Kim, Moonjeong; Jeon, Jinseong; Han, Jin Kwan; Kim, Kwang-Sun

    2017-08-26

    Outer membrane vesicles (OMVs) derived from bacteria are promising candidates for subunit vaccines. Stresses that modulate the composition of outer membrane proteins (OMPs) are important for OMV synthesis. Small RNAs (sRNAs) expressed in response to stress regulate OMPs, although the mechanism underlying sRNA-mediated OMV biogenesis and its utility for developing vaccine platforms remains to be elucidated. Here, we characterized the role of a sRNA, MicA, which regulates OmpA, a major OMP involved in both production of OMVs and reactive immunity against Salmonella challenge. A Salmonella strain overexpressing MicA generated more OMVs than a control strain. In addition, OmpC was the major component of MicA-derived OMV proteins. MicA-derived OMVs induced Th1- and Th17-type immune responses in vitro and reduced Salmonella-mediated lethality in a mouse model. Thus, OmpA-regulatory sRNA-derived OMVs may facilitate production of Salmonella-protective vaccines. Copyright © 2017 Elsevier Inc. All rights reserved.

  12. Development of Power Assisting Suit

    Science.gov (United States)

    Yamamoto, Keijiro; Ishii, Mineo; Hyodo, Kazuhito; Yoshimitsu, Toshihiro; Matsuo, Takashi

    In order to realize a wearable power assisting suit for assisting a nurse to carry a patient in her arms, the power supply and control systems of the suit have to be miniaturized, and it has to be wireless and pipeline-less. The new wearable suit consists of shoulders, arms, back, waist and legs units to be fitted on the nurse's body. The arms, waist and legs have new pneumatic rotary actuators driven directly by micro air pumps supplied by portable Ni-Cd batteries. The muscle forces are sensed by a new muscle hardness sensor utilizing a sensing tip mounted on a force sensing film device. An embedded microcomputer is used for the calculations of control signals. The new wearable suit was applied practically to a human body and a series of movement experiments that weights in the arms were held and taken up and down was performed. Each unit of the suit could transmit assisting torque directly to each joint verifying its practicability.

  13. The outer membrane protein Omp35 affects the reduction of Fe(III, nitrate, and fumarate by Shewanella oneidensis MR-1

    Directory of Open Access Journals (Sweden)

    Myers Charles R

    2004-06-01

    Full Text Available Abstract Background Shewanella oneidensis MR-1 uses several electron acceptors to support anaerobic respiration including insoluble species such as iron(III and manganese(IV oxides, and soluble species such as nitrate, fumarate, dimethylsulfoxide and many others. MR-1 has complex branched electron transport chains that include components in the cytoplasmic membrane, periplasm, and outer membrane (OM. Previous studies have implicated a role for anaerobically upregulated OM electron transport components in the use of insoluble electron acceptors, and have suggested that other OM components may also contribute to insoluble electron acceptor use. In this study, the role for an anaerobically upregulated 35-kDa OM protein (Omp35 in the use of anaerobic electron acceptors was explored. Results Omp35 was purified from the OM of anaerobically grown cells, the gene encoding Omp35 was identified, and an omp35 null mutant (OMP35-1 was isolated and characterized. Although OMP35-1 grew on all electron acceptors tested, a significant lag was seen when grown on fumarate, nitrate, and Fe(III. Complementation studies confirmed that the phenotype of OMP35-1 was due to the loss of Omp35. Despite its requirement for wild-type rates of electron acceptor use, analysis of Omp35 protein and predicted sequence did not identify any electron transport moieties or predicted motifs. OMP35-1 had normal levels and distribution of known electron transport components including quinones, cytochromes, and fumarate reductase. Omp35 is related to putative porins from MR-1 and S. frigidimarina as well as to the PorA porin from Neisseria meningitidis. Subcellular fraction analysis confirmed that Omp35 is an OM protein. The seven-fold anaerobic upregulation of Omp35 is mediated post-transcriptionally. Conclusion Omp35 is a putative porin in the OM of MR-1 that is markedly upregulated anaerobically by a post-transcriptional mechanism. Omp35 is required for normal rates of growth on Fe

  14. A FRET-based DNA biosensor tracks OmpR-dependent acidification of Salmonella during macrophage infection.

    Directory of Open Access Journals (Sweden)

    Smarajit Chakraborty

    2015-04-01

    Full Text Available In bacteria, one paradigm for signal transduction is the two-component regulatory system, consisting of a sensor kinase (usually a membrane protein and a response regulator (usually a DNA binding protein. The EnvZ/OmpR two-component system responds to osmotic stress and regulates expression of outer membrane proteins. In Salmonella, EnvZ/OmpR also controls expression of another two-component system SsrA/B, which is located on Salmonella Pathogenicity Island (SPI 2. SPI-2 encodes a type III secretion system, which functions as a nanomachine to inject bacterial effector proteins into eukaryotic cells. During the intracellular phase of infection, Salmonella switches from assembling type III secretion system structural components to secreting effectors into the macrophage cytoplasm, enabling Salmonella to replicate in the phagocytic vacuole. Major questions remain regarding how bacteria survive the acidified vacuole and how acidification affects bacterial secretion. We previously reported that EnvZ sensed cytoplasmic signals rather than extracellular ones, as intracellular osmolytes altered the dynamics of a 17-amino-acid region flanking the phosphorylated histidine. We reasoned that the Salmonella cytoplasm might acidify in the macrophage vacuole to activate OmpR-dependent transcription of SPI-2 genes. To address these questions, we employed a DNA-based FRET biosensor ("I-switch" to measure bacterial cytoplasmic pH and immunofluorescence to monitor effector secretion during infection. Surprisingly, we observed a rapid drop in bacterial cytoplasmic pH upon phagocytosis that was not predicted by current models. Cytoplasmic acidification was completely dependent on the OmpR response regulator, but did not require known OmpR-regulated genes such as ompC, ompF, or ssaC (SPI-2. Microarray analysis highlighted the cadC/BA operon, and additional experiments confirmed that it was repressed by OmpR. Acidification was blocked in the ompR null background in a

  15. Suited Contingency Ops Food - 2

    Science.gov (United States)

    Glass, J. W.; Leong, M. L.; Douglas, G. L.

    2014-01-01

    The contingency scenario for an emergency cabin depressurization event may require crewmembers to subsist in a pressurized suit for up to 144 hours. This scenario requires the capability for safe nutrition delivery through a helmet feed port against a 4 psi pressure differential to enable crewmembers to maintain strength and cognition to perform critical tasks. Two nutritional delivery prototypes were developed and analyzed for compatibility with the helmet feed port interface and for operational effectiveness against the pressure differential. The bag-in-bag (BiB) prototype, designed to equalize the suit pressure with the beverage pouch and enable a crewmember to drink normally, delivered water successfully to three different subjects in suits pressurized to 4 psi. The Boa restrainer pouch, designed to provide mechanical leverage to overcome the pressure differential, did not operate sufficiently. Guidelines were developed and compiled for contingency beverages that provide macro-nutritional requirements, a minimum one-year shelf life, and compatibility with the delivery hardware. Evaluation results and food product parameters have the potential to be used to improve future prototype designs and develop complete nutritional beverages for contingency events. These feeding capabilities would have additional use on extended surface mission EVAs, where the current in-suit drinking device may be insufficient.

  16. The ZPIC educational code suite

    Science.gov (United States)

    Calado, R.; Pardal, M.; Ninhos, P.; Helm, A.; Mori, W. B.; Decyk, V. K.; Vieira, J.; Silva, L. O.; Fonseca, R. A.

    2017-10-01

    Particle-in-Cell (PIC) codes are used in almost all areas of plasma physics, such as fusion energy research, plasma accelerators, space physics, ion propulsion, and plasma processing, and many other areas. In this work, we present the ZPIC educational code suite, a new initiative to foster training in plasma physics using computer simulations. Leveraging on our expertise and experience from the development and use of the OSIRIS PIC code, we have developed a suite of 1D/2D fully relativistic electromagnetic PIC codes, as well as 1D electrostatic. These codes are self-contained and require only a standard laptop/desktop computer with a C compiler to be run. The output files are written in a new file format called ZDF that can be easily read using the supplied routines in a number of languages, such as Python, and IDL. The code suite also includes a number of example problems that can be used to illustrate several textbook and advanced plasma mechanisms, including instructions for parameter space exploration. We also invite contributions to this repository of test problems that will be made freely available to the community provided the input files comply with the format defined by the ZPIC team. The code suite is freely available and hosted on GitHub at https://github.com/zambzamb/zpic. Work partially supported by PICKSC.

  17. Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk Proteins

    Science.gov (United States)

    Ibañez, Andrés E.; Smaldini, Paola; Coria, Lorena M.; Delpino, María V.; Pacífico, Lucila G. G.; Oliveira, Sergio C.; Risso, Gabriela S.; Pasquevich, Karina A.; Fossati, Carlos Alberto; Giambartolomei, Guillermo H.; Docena, Guillermo H.; Cassataro, Juliana

    2013-01-01

    The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVA-specific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow’s Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations. PMID:23861971

  18. Enabling interoperability in Geoscience with GI-suite

    Science.gov (United States)

    Boldrini, Enrico; Papeschi, Fabrizio; Santoro, Mattia; Nativi, Stefano

    2015-04-01

    GI-suite is a brokering framework targeting interoperability of heterogeneous systems in the Geoscience domain. The framework is composed by different brokers each one focusing on a specific functionality: discovery, access and semantics (i.e. GI-cat, GI-axe, GI-sem). The brokering takes place between a set of heterogeneous publishing services and a set of heterogeneous consumer applications: the brokering target is represented by resources (e.g. coverages, features, or metadata information) required to seamlessly flow from the providers to the consumers. Different international and community standards are now supported by GI-suite, making possible the successful deployment of GI-suite in many international projects and initiatives (such as GEOSS, NSF BCube and several EU funded projects). As for the publisher side more than 40 standards and implementations are supported (e.g. Dublin Core, OAI-PMH, OGC W*S, Geonetwork, THREDDS Data Server, Hyrax Server, etc.). The support for each individual standard is provided by means of specific GI-suite components, called accessors. As for the consumer applications side more than 15 standards and implementations are supported (e.g. ESRI ArcGIS, Openlayers, OGC W*S, OAI-PMH clients, etc.). The support for each individual standard is provided by means of specific profiler components. The GI-suite can be used in different scenarios by different actors: - A data provider having a pre-existent data repository can deploy and configure GI-suite to broker it and making thus available its data resources through different protocols to many different users (e.g. for data discovery and/or data access) - A data consumer can use GI-suite to discover and/or access resources from a variety of publishing services that are already publishing data according to well-known standards. - A community can deploy and configure GI-suite to build a community (or project-specific) broker: GI-suite can broker a set of community related repositories and

  19. Structure refinement and membrane positioning of selectively labeled OmpX in phospholipid nanodiscs

    Energy Technology Data Exchange (ETDEWEB)

    Hagn, Franz, E-mail: franz.hagn@tum.de; Wagner, Gerhard, E-mail: gerhard-wagner@hms.harvard.edu [Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology (United States)

    2015-04-15

    NMR structural studies on membrane proteins are often complicated by their large size, taking into account the contribution of the membrane mimetic. Therefore, classical resonance assignment approaches often fail. The large size of phospholipid nanodiscs, a detergent-free phospholipid bilayer mimetic, prevented their use in high-resolution solution-state NMR spectroscopy so far. We recently introduced smaller nanodiscs that are suitable for NMR structure determination. However, side-chain assignments of a membrane protein in nanodiscs still remain elusive. Here, we utilized a NOE-based approach to assign (stereo-) specifically labeled Ile, Leu, Val and Ala methyl labeled and uniformly {sup 15}N-Phe and {sup 15}N-Tyr labeled OmpX and calculated a refined high-resolution structure. In addition, we were able to obtain residual dipolar couplings (RDCs) of OmpX in nanodiscs using Pf1 phage medium for the induction of weak alignment. Back-calculated NOESY spectra of the obtained NMR structures were compared to experimental NOESYs in order to validate the quality of these structures. We further used NOE information between protonated lipid head groups and side-chain methyls to determine the position of OmpX in the phospholipid bilayer. These data were verified by paramagnetic relaxation enhancement (PRE) experiments obtained with Gd{sup 3+}-modified lipids. Taken together, this study emphasizes the need for the (stereo-) specific labeling of membrane proteins in a highly deuterated background for high-resolution structure determination, particularly in large membrane mimicking systems like phospholipid nanodiscs. Structure validation by NOESY back-calculation will be helpful for the structure determination and validation of membrane proteins where NOE assignment is often difficult. The use of protein to lipid NOEs will be beneficial for the positioning of a membrane protein in the lipid bilayer without the need for preparing multiple protein samples.

  20. Efficient Programming for Multicore Processor Heterogeneity: OpenMP versus OmpSs

    OpenAIRE

    Butko , Anastasiia; Bruguier , Florent; Gamatié , Abdoulaye; Sassatelli , Gilles

    2017-01-01

    International audience; ARM single-ISA heterogeneous multicore processors combine high-performance big cores with power-efficient small cores. They aim at achieving a suitable balance between performance and energy. How- ever, a main challenge is to program such architectures so as to efficiently exploit their features. In this paper, we study the impact on performance and energy trade-offs of single-ISA architecture according to OpenMP 3.0 and the OmpSs programming models. We consider differ...

  1. Accelerated microevolution in an outer membrane protein (OMP of the intracellular bacteria Wolbachia

    Directory of Open Access Journals (Sweden)

    Russell Jacob A

    2010-02-01

    Full Text Available Abstract Background Outer membrane proteins (OMPs of Gram-negative bacteria are key players in the biology of bacterial-host interactions. However, while considerable attention has been given to OMPs of vertebrate pathogens, relatively little is known about the role of these proteins in bacteria that primarily infect invertebrates. One such OMP is found in the intracellular bacteria Wolbachia, which are widespread symbionts of arthropods and filarial nematodes. Recent experimental studies have shown that the Wolbachia surface protein (WSP can trigger host immune responses and control cell death programming in humans, suggesting a key role of WSP for establishment and persistence of the symbiosis in arthropods. Results Here we performed an analysis of 515 unique alleles found in 831 Wolbachia isolates, to investigate WSP structure, microevolution and population genetics. WSP shows an eight-strand transmembrane β-barrel structure with four extracellular loops containing hypervariable regions (HVRs. A clustering approach based upon patterns of HVR haplotype diversity was used to group similar WSP sequences and to estimate the relative contribution of mutation and recombination during early stages of protein divergence. Results indicate that although point mutations generate most of the new protein haplotypes, recombination is a predominant force triggering diversity since the very first steps of protein evolution, causing at least 50% of the total amino acid variation observed in recently diverged proteins. Analysis of synonymous variants indicates that individual WSP protein types are subject to a very rapid turnover and that HVRs can accommodate a virtually unlimited repertoire of peptides. Overall distribution of WSP across hosts supports a non-random association of WSP with the host genus, although extensive horizontal transfer has occurred also in recent times. Conclusions In OMPs of vertebrate pathogens, large recombination impact, positive

  2. An OmpA Family Protein, a Target of the GinI/GinR Quorum-Sensing System in Gluconacetobacter intermedius, Controls Acetic Acid Fermentation▿ †

    Science.gov (United States)

    Iida, Aya; Ohnishi, Yasuo; Horinouchi, Sueharu

    2008-01-01

    Via N-acylhomoserine lactones, the GinI/GinR quorum-sensing system in Gluconacetobacter intermedius NCI1051, a gram-negative acetic acid bacterium, represses acetic acid and gluconic acid fermentation. Two-dimensional polyacrylamide gel electrophoretic analysis of protein profiles of strain NCI1051 and ginI and ginR mutants identified a protein that was produced in response to the GinI/GinR regulatory system. Cloning and nucleotide sequencing of the gene encoding this protein revealed that it encoded an OmpA family protein, named GmpA. gmpA was a member of the gene cluster containing three adjacent homologous genes, gmpA to gmpC, the organization of which appeared to be unique to vinegar producers, including “Gluconacetobacter polyoxogenes.” In addition, GmpA was unique among the OmpA family proteins in that its N-terminal membrane domain forming eight antiparallel transmembrane β-strands contained an extra sequence in one of the surface-exposed loops. Transcriptional analysis showed that only gmpA of the three adjacent gmp genes was activated by the GinI/GinR quorum-sensing system. However, gmpA was not controlled directly by GinR but was controlled by an 89-amino-acid protein, GinA, a target of this quorum-sensing system. A gmpA mutant grew more rapidly in the presence of 2% (vol/vol) ethanol and accumulated acetic acid and gluconic acid in greater final yields than strain NCI1051. Thus, GmpA plays a role in repressing oxidative fermentation, including acetic acid fermentation, which is unique to acetic acid bacteria and allows ATP synthesis via ethanol oxidation. Consistent with the involvement of gmpA in oxidative fermentation, its transcription was also enhanced by ethanol and acetic acid. PMID:18487322

  3. An OmpA family protein, a target of the GinI/GinR quorum-sensing system in Gluconacetobacter intermedius, controls acetic acid fermentation.

    Science.gov (United States)

    Iida, Aya; Ohnishi, Yasuo; Horinouchi, Sueharu

    2008-07-01

    Via N-acylhomoserine lactones, the GinI/GinR quorum-sensing system in Gluconacetobacter intermedius NCI1051, a gram-negative acetic acid bacterium, represses acetic acid and gluconic acid fermentation. Two-dimensional polyacrylamide gel electrophoretic analysis of protein profiles of strain NCI1051 and ginI and ginR mutants identified a protein that was produced in response to the GinI/GinR regulatory system. Cloning and nucleotide sequencing of the gene encoding this protein revealed that it encoded an OmpA family protein, named GmpA. gmpA was a member of the gene cluster containing three adjacent homologous genes, gmpA to gmpC, the organization of which appeared to be unique to vinegar producers, including "Gluconacetobacter polyoxogenes." In addition, GmpA was unique among the OmpA family proteins in that its N-terminal membrane domain forming eight antiparallel transmembrane beta-strands contained an extra sequence in one of the surface-exposed loops. Transcriptional analysis showed that only gmpA of the three adjacent gmp genes was activated by the GinI/GinR quorum-sensing system. However, gmpA was not controlled directly by GinR but was controlled by an 89-amino-acid protein, GinA, a target of this quorum-sensing system. A gmpA mutant grew more rapidly in the presence of 2% (vol/vol) ethanol and accumulated acetic acid and gluconic acid in greater final yields than strain NCI1051. Thus, GmpA plays a role in repressing oxidative fermentation, including acetic acid fermentation, which is unique to acetic acid bacteria and allows ATP synthesis via ethanol oxidation. Consistent with the involvement of gmpA in oxidative fermentation, its transcription was also enhanced by ethanol and acetic acid.

  4. Methylation and in vivo expression of the surface-exposed Leptospira interrogans outer-membrane protein OmpL32.

    Science.gov (United States)

    Eshghi, Azad; Pinne, Marija; Haake, David A; Zuerner, Richard L; Frank, Ami; Cameron, Caroline E

    2012-03-01

    Recent studies have revealed that bacterial protein methylation is a widespread post-translational modification that is required for virulence in selected pathogenic bacteria. In particular, altered methylation of outer-membrane proteins has been shown to modulate the effectiveness of the host immune response. In this study, 2D gel electrophoresis combined with MALDI-TOF MS identified a Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 protein, corresponding to ORF LIC11848, which undergoes extensive and differential methylation of glutamic acid residues. Immunofluorescence microscopy implicated LIC11848 as a surface-exposed outer-membrane protein, prompting the designation OmpL32. Indirect immunofluorescence microscopy of golden Syrian hamster liver and kidney sections revealed expression of OmpL32 during colonization of these organs. Identification of methylated surface-exposed outer-membrane proteins, such as OmpL32, provides a foundation for delineating the role of this post-translational modification in leptospiral virulence.

  5. Ducks as a potential reservoir for Pasteurella multocida infection detected using a new rOmpH-based ELISA.

    Science.gov (United States)

    Liu, Rongchang; Chen, Cuiteng; Cheng, Longfei; Lu, Ronghui; Fu, Guanghua; Shi, Shaohua; Chen, Hongmei; Wan, Chunhe; Lin, Jiansheng; Fu, Qiuling; Huang, Yu

    2017-07-28

    Pasteurella multocida is an important pathogen of numerous domestic poultry and wild animals and is associated with a variety of diseases including fowl cholera. The aim of this study was to develop an indirect enzyme-linked immunosorbent assay (ELISA) based on recombinant outer-membrane protein H (rOmpH) for detection of anti-P. multocida antibodies in serum to determine their prevalence in Chinese ducks. The P. multocida ompH gene was cloned into pET32a, and rOmpH was expressed in Escherichia coli BL21 (DE3). Western blotting revealed that purified rOmpH was recognized by duck antisera against P. multocida, and an indirect ELISA was established. During analysis of serum samples (n=115) from ducks, the rOmpH ELISA showed 95.0% specificity, 100% sensitivity and a 92.0% κ coefficient (95% confidence interval 0.844-0.997) as compared with a microtiter agglutination test. Among 165 randomly selected serum samples, which were collected in 2015 and originated from six duck farms across Fujian Province, China, anti-P. multocida antibodies were detected in 22.42% of apparently healthy ducks, including 25 of 90 sheldrakes (27.8%), eight of 50 Peking ducks (16.0%) and four of 25 Muscovy ducks (16%). Overall, the data suggest that rOmpH is a suitable candidate antigen for the development of an indirect ELISA for detection of P. multocida in ducks; moreover, our results showed that ducks could serve as a potential reservoir for P. multocida infection.

  6. MzrA: a novel modulator of the EnvZ/OmpR two-component regulon

    Science.gov (United States)

    Gerken, Henri; Charlson, Emily S; Cicirelli, Elisha M; Kenney, Linda J; Misra, Rajeev

    2009-01-01

    Analysis of suppressors that alleviate the acute envelope stress phenotype of a ΔbamBΔdegP strain of Escherichia coli identified a novel protein MzrA and pleiotropic envZ mutations. Genetic evidence shows that overexpression of MzrA – formerly known as YqjB and EcfM – modulates the activity of EnvZ/OmpR similarly to pleiotropic EnvZ mutants and alter porin expression. However, porin expression in strains devoid of MzrA or overexpressing it is still sensitive to medium osmolarity, pH and procaine, all of which modulate EnvZ/OmpR activities. Thus, MzrA appears to alter the output of the EnvZ/OmpR system but not its ability to receive and respond to various environmental signals. Localization and topology experiments indicate that MzrA is a type II membrane protein, with its N-terminus exposed in the cytoplasm and C-terminus in the periplasm. Bacterial two-hybrid experiments determined that MzrA specifically interacts with EnvZ but not with OmpR or the related membrane sensor kinase, CpxA. This and additional genetic and biochemical evidence suggest that the interaction of MzrA with EnvZ would either enhance EnvZ's kinase activity or reduce its phosphatase activity, thus elevating the steady state levels of OmpR∼P. Furthermore, our data show that MzrA links the two-component envelope stress response regulators, CpxA/CpxR and EnvZ/OmpR. PMID:19432797

  7. Serum Anti-Vibrio cholerae Immunoglobulin Isotype in BALB/c Mice Immunized With ompW-Loaded Chitosan

    Directory of Open Access Journals (Sweden)

    Mahdi Fasihi-Ramandi

    2016-05-01

    Full Text Available Background: Chitosan, a liner polysaccharide, is a biocompatible and safe material for the delivery of therapeutic proteins and antigens, particularly via mucosal systems. Objectives: In this study, the production of antibodies in response to outermembrane protein W (ompW-loaded chitosan in BALB/c mice was evaluated. Materials and Methods: Mice were subjected to intraperitoneal injection of ompW or nasal administration of ompW-loaded chitosan on days 1, 14, and 28, and the antibodies were measured on day 42 with ELISA. Results: The titration of antibodies indicated that the nasal administration of ompW-loaded chitosan was better able to stimulate the immune response compared to intraperitoneal injections. However, the titration of total and IgG isotypes showed a significant difference between intraperitoneal and nasal immunization (P < 0.01. A significant difference was also seen in serum IgA isotypes at over 1/80 titrations, but not at lower dilutions (P < 0.01. Despite the serum antibodies, the results of lavage fluid analysis revealed that the IgG and IgA isotypes in the mice subjected to nasal immunization with ompW-loaded chitosan were significantly higher than in the other group (P < 0.01. Conclusions: Based on the preliminary results presented in this research, it is suggested that ompW-loaded chitosan could be a suitable choice for nasal application to immunize the host against Vibrio cholerae. However, more work is required to determine the efficiency of the antibodies in neutralizing the bacterial toxin or bacterial movement.

  8. Host factor I, Hfq, binds to Escherichia coli ompA mRNA in a growth rate-dependent fashion and regulates its stability

    DEFF Research Database (Denmark)

    Vytvytska, O; Jakobsen, J S; Balcunaite, G

    1998-01-01

    RNA. In hfq mutant cells with a deficient Hfq gene product, the RNA-binding activity is missing, and analysis of the ompA mRNA showed that the growth-rate dependence of degradation is lost. Furthermore, the half-life of the ompA mRNA is prolonged in the mutant cells, irrespective of growth rate. Hfq has...

  9. Sequencing of the Chlamydophila psittaci ompA Gene Reveals a New Genotype, E/B, and the Need for a Rapid Discriminatory Genotyping Method

    Science.gov (United States)

    Geens, Tom; Desplanques, Ann; Van Loock, Marnix; Bönner, Brigitte M.; Kaleta, Erhard F.; Magnino, Simone; Andersen, Arthur A.; Everett, Karin D. E.; Vanrompay, Daisy

    2005-01-01

    Twenty-one avian Chlamydophila psittaci isolates from different European countries were characterized using ompA restriction fragment length polymorphism, ompA sequencing, and major outer membrane protein serotyping. Results reveal the presence of a new genotype, E/B, in several European countries and stress the need for a discriminatory rapid genotyping method. PMID:15872282

  10. Export-defective lamB protein is a target for translational control caused by ompC porin overexpression.

    OpenAIRE

    Click, E M; Schnaitman, C A

    1989-01-01

    Overexpression of OmpC protein from an inducible plasmid vector reduced the amount of the precursor form of LamB protein in LamB signal sequence mutants. The stability of the precursor form of LamB protein was not affected, indicating that the effect of OmpC overexpression was on the synthesis of the precursor rather than on degradation. These results indicate that a functional signal sequence is not required on an outer membrane protein for it to be a target for translational control.

  11. Data Continuity of Aerosol Index from Suomi NPP/OMPS Observations

    Science.gov (United States)

    Ahn, C.; Torres, O.; Tiruchirapalli, R.; Taylor, S.; Jethva, H. T.

    2017-12-01

    Since the development of the Aerosol Index (AI) concept from Nimubs-7 TOMS near-UV measurements, the AI product has been widely used by the aerosol community in a variety of applications including monitoring of the sources and sinks of carbonaceous and desert dust aerosols. The AI uses a pair of near-UV radiances to detect the presence of absorbing particles even over bright backgrounds such as clouds and snow/ice covered areas. Since its inception in the mid 90's, the AI has been available as a by-product of the total ozone product. Due to the implementation of a new total ozone algorithm, the standard AI product will no longer be available starting in 2018. To assure the continuity of the AI record, we have developed an improved AI algorithm that uses a better forward modeling method of the top of atmosphere radiances. The enhanced modelling capability accounts for the scattering of clouds using Mie theory, and includes the effect of wavelength and angle dependent surface reflectance effects. By doing this, we have significantly reduced angular dependent false AI signals such as sun glint over the ocean. We will discuss the improved AI algorithm and present the long term AI record from various UV space borne sensors including TOMS, OMI, OMPS, and EPIC with consistent AI algorithms, followed by future plans for near-real time processing and operational production of a new OMPS AI product.

  12. Comprehensive Benchmark Suite for Simulation of Particle Laden Flows Using the Discrete Element Method with Performance Profiles from the Multiphase Flow with Interface eXchanges (MFiX) Code

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Peiyuan [Univ. of Colorado, Boulder, CO (United States); Brown, Timothy [Univ. of Colorado, Boulder, CO (United States); Fullmer, William D. [Univ. of Colorado, Boulder, CO (United States); Hauser, Thomas [Univ. of Colorado, Boulder, CO (United States); Hrenya, Christine [Univ. of Colorado, Boulder, CO (United States); Grout, Ray [National Renewable Energy Lab. (NREL), Golden, CO (United States); Sitaraman, Hariswaran [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-01-29

    Five benchmark problems are developed and simulated with the computational fluid dynamics and discrete element model code MFiX. The benchmark problems span dilute and dense regimes, consider statistically homogeneous and inhomogeneous (both clusters and bubbles) particle concentrations and a range of particle and fluid dynamic computational loads. Several variations of the benchmark problems are also discussed to extend the computational phase space to cover granular (particles only), bidisperse and heat transfer cases. A weak scaling analysis is performed for each benchmark problem and, in most cases, the scalability of the code appears reasonable up to approx. 103 cores. Profiling of the benchmark problems indicate that the most substantial computational time is being spent on particle-particle force calculations, drag force calculations and interpolating between discrete particle and continuum fields. Hardware performance analysis was also carried out showing significant Level 2 cache miss ratios and a rather low degree of vectorization. These results are intended to serve as a baseline for future developments to the code as well as a preliminary indicator of where to best focus performance optimizations.

  13. Transcriptional regulation of the outer membrane porin gene ompW reveals its physiological role during the transition from the aerobic to the anaerobic lifestyle of Escherichia coli

    Directory of Open Access Journals (Sweden)

    Minfeng eXiao

    2016-05-01

    Full Text Available Understanding bacterial physiology relies on elucidating the regulatory mechanisms and cellular functions of those differentially expressed genes in response to environmental changes. A widespread Gram-negative bacterial outer membrane protein OmpW has been implicated in the adaptation to stresses in various species. It is recently found to be present in the regulon of the global anaerobic transcription factor FNR and ArcA in E. coli. However, little is known about the physiological implications of this regulatory disposition. In this study, we demonstrate that transcription of ompW is indeed mediated by a series of global regulators involved in the anaerobiosis of E. coli. We show that FNR can both activate and repress the expression of ompW through its direct binding to two distinctive sites, -81.5 and -126.5 bp respectively, on ompW promoter. ArcA also participates in repression of ompW under anaerobic condition, but in an FNR dependent manner. Additionally, ompW is also subject to the regulation by CRP and NarL which senses the availability and types of carbon sources and respiration electron acceptors in the environment respectively, implying a role of OmpW in the carbon and energy metabolism of E. coli during its anaerobic adaptation. Molecular docking reveals that OmpW can bind fumarate, an alternative electron acceptor in anaerobic respiration, with sufficient affinity. Moreover, supplement of fumarate or succinate which belongs to the C4-dicarboxylates family of metabolite, to E. coli culture rescues OmpW-mediated colicin S4 killing. Taken together, we propose that OmpW is involved in anaerobic carbon and energy metabolism to mediate the transition from aerobic to anaerobic lifestyle in E. coli.

  14. Genotyping of Pasteurella multocida ovine and bovine isolates from Iran based on PCR-RFLP of ompH gene

    Directory of Open Access Journals (Sweden)

    A. Ghanizadeh

    2015-10-01

    Full Text Available Pasteurella multocida (P. multocida, A Gram-negative facultative anaerobic bacterium, is a causative animal pathogen in porcine atrophic rhinitis and avian fowl cholera. The outer membrane of Gram-negative bacteria contains of many different protein in very high copy numbers. One of the major outer membrane, the H proteins have functional as high immunogenicity and antigenicity. In this study to increase information about epidemiology of ovine and bovine P. multocida, the 24 isolates from sheep and nine isolates from cattle were investigated by PCR-RFLP analysis of the ompH gene. In all 33 isolates, digestion of the amplified fragment of ompH gene by using EcoRI, cfoI and HindIII produced 3, 5 and 3 different restriction patterns respectively. Sixteen RFLP patterns were found among 33 investigated P.multocida isolates. This study showed that, the PCR RFLP based on ompH gene is potentially a useful method for typing of P. multocida isolates from sheep and cattle. The RFLP patterns of this gene exhibited extensive restriction site heterogeneity, which may be particularly suitable for fingerprinting of P. multocida isolates.Considering ompH protein as a protective immunogenic moiety of P.ultocida, the results of this study showed a heterogenic bacteria and this means the possibility to produce a multivalent vaccine to be protective against diseases caused by this organism in sheep and cattle in Iran.

  15. Expression, crystallization and preliminary X-ray crystallographic studies of the outer membrane protein OmpW from Escherichia coli

    International Nuclear Information System (INIS)

    Albrecht, Reinhard; Zeth, Kornelius; Söding, Johannes; Lupas, Andrei; Linke, Dirk

    2006-01-01

    The outer membrane protein OmpW from E. coli was overexpressed in inclusion bodies and refolded with the help of detergent. The protein has been crystallized and the crystals diffract to 3.5 Å resolution. OmpW is an eight-stranded 21 kDa molecular-weight β-barrel protein from the outer membrane of Gram-negative bacteria. It is a major antigen in bacterial infections and has implications in antibiotic resistance and in the oxidative degradation of organic compounds. OmpW from Escherichia coli was cloned and the protein was expressed in inclusion bodies. A method for refolding and purification was developed which yields properly folded protein according to circular-dichroism measurements. The protein has been crystallized and crystals were obtained that diffracted to a resolution limit of 3.5 Å. The crystals belong to space group P422, with unit-cell parameters a = 122.5, c = 105.7 Å. A homology model of OmpW is presented based on known structures of eight-stranded β-barrels, intended for use in molecular-replacement trials

  16. Safety in the use of pressurized suits

    International Nuclear Information System (INIS)

    1984-01-01

    This Code of Practice describes the procedures relating to the safe operation of Pressurized Suit Areas and their supporting services. It is directed at personnel responsible for the design and/or operation of Pressurized Suit Areas. (author)

  17. The OmpA-like protein Loa22 is essential for leptospiral virulence.

    Directory of Open Access Journals (Sweden)

    Paula Ristow

    2007-07-01

    Full Text Available Pathogenic mechanisms of Leptospira interrogans, the causal agent of leptospirosis, remain largely unknown. This is mainly due to the lack of tools for genetic manipulations of pathogenic species. In this study, we characterized a mutant obtained by insertion of the transposon Himar1 into a gene encoding a putative lipoprotein, Loa22, which has a predicted OmpA domain based on sequence identity. The resulting mutant did not express Loa22 and was attenuated in virulence in the guinea pig and hamster models of leptospirosis, whereas the genetically complemented strain was restored in Loa22 expression and virulence. Our results show that Loa22 was expressed during host infection and exposed on the cell surface. Loa22 is therefore necessary for virulence of L. interrogans in the animal model and represents, to our knowledge, the first genetically defined virulence factor in Leptospira species.

  18. Z-2 Suit Support Stand and MKIII Suit Center of Gravity Test

    Science.gov (United States)

    Nguyen, Tuan Q.

    2014-01-01

    NASA's next generation spacesuits are the Z-Series suits, made for a range of possible exploration missions in the near future. The prototype Z-1 suit has been developed and assembled to incorporate new technologies that has never been utilized before in the Apollo suits and the Extravehicular Mobility Unit (EMU). NASA engineers tested the Z-1 suit extensively in order to developed design requirements for the new Z-2 suit. At the end of 2014, NASA will be receiving the new Z-2 suit to perform more testing and to further develop the new technologies of the suit. In order to do so, a suit support stand will be designed and fabricated to support the Z-2 suit during maintenance, sizing, and structural leakage testing. The Z-2 Suit Support Stand (Z2SSS) will be utilized for these purposes in the early testing stages of the Z-2 suit.

  19. Chicken IgY Fc linked to Bordetella avium ompA and Taishan Pinus massoniana pollen polysaccharide adjuvant enhances macrophage function and specific immune responses

    Directory of Open Access Journals (Sweden)

    Zhu Ruiliang

    2016-11-01

    Full Text Available Fc-fusion technologies, in which immunoglobulin Fc is genetically fused to an antigenic protein, have been developed to confer antibody-like properties to proteins and peptides. Mammalian IgG Fc fusion exhibits improved antigen-induced immune responses by providing aggregates with high avidity for the IgG Fc receptor and salvaging the antigenic portion from endosomal degradation. However, whether the linked chicken IgY Fc fragment shares similar characteristics to mammalian IgG Fc remains unclear. In this study, we linked the chicken IgY Fc gene to the outer membrane protein A (ompA of Borderella avium through overlapping PCR. The fusion gene was cloned into the pPIC9 plasmid to construct the recombinant Pichia pastoris transformant expressing the ompA–Fc fusion protein. The effects of the linked Fc on macrophage vitality, activity, efficiency of antigen processing, and immune responses induced by the fused ompA were investigated. Furthermore, the effect of Taishan Pinus massoniana pollen polysaccharide (TPPPS, an immunomodulator, on chicken macrophage activation was evaluated. TPPPS was also used as an adjuvant to investigate its immunomodulatory effect on immunoresponses induced by the fused ompA–Fc in chickens. The pinocytosis, phagocytosis, secretion of nitric oxide and TNF-α, and MHC-II molecular expression of the macrophages treated with the fused ompA–Fc were significantly higher than those of the macrophages treated with ompA alone. The addition of TPPPS to the fused ompA–Fc further enhanced macrophage functions. The fused ompA–Fc elicited higher antigen-specific immune responses and protective efficacy compared with ompA alone. Moreover, the fused ompA–Fc conferred higher serum antibody titers, serum IL-2 and IL-4 concentrations, CD4+ and CD8+ T-lymphocyte counts, lymphocyte transformation rate, and protection rate compared with ompA alone. Notably, the prepared TPPPS adjuvant ompA–Fc vaccines induced high immune

  20. Influenza viral vectors expressing the Brucella OMP16 or L7/L12 proteins as vaccines against B. abortus infection.

    Science.gov (United States)

    Tabynov, Kaissar; Sansyzbay, Abylai; Kydyrbayev, Zhailaubay; Yespembetov, Bolat; Ryskeldinova, Sholpan; Zinina, Nadezhda; Assanzhanova, Nurika; Sultankulova, Kulaisan; Sandybayev, Nurlan; Khairullin, Berik; Kuznetsova, Irina; Ferko, Boris; Egorov, Andrej

    2014-04-10

    We generated novel, effective candidate vaccine against Brucella abortus based on recombinant influenza viruses expressing the Brucella ribosomal protein L7/L12 or outer membrane protein (Omp)-16 from the NS1 open reading frame. The main purpose of this work was to evaluate the safety, immunogenicity and protectiveness of vaccine candidate in laboratory animals. Four recombinant influenza A viral constructs of the subtypes Н5N1 or H1N1 expressing the Brucella proteins L7/L12 or Omp16 were obtained by a reverse genetics method: Flu-NS1-124-L7/L12-H5N1, Flu-NS1-124-Omp16-H5N1, Flu-NS1-124-L7/L12-H1N1 and Flu-NS1-124-Omp16-H1N1. Despite of substantial modification of NS1 gene, all constructs replicated well and were retain their Brucella inserts over five passages in embryonated chicken eggs (CE). Administration of the mono- or bivalent vaccine formulation via prime-boost intranasal (i.n.), conjunctival (c.) or subcutaneous (s.c.) immunization was safe in mice; no deaths, body weight loss or pathomorphological changes were observed over 56 days. Moreover, guinea pigs vaccinated i.n. with vaccine vectors did not shed the vaccine viruses through their upper respiratory tract after the prime and booster vaccination. These findings confirmed the replication-deficient phenotype of viral vectors. The highest antibody response to Brucella antigen was obtained with constructs expressing L7/L12 (ELISA, GMT 242.5-735.0); whereas the highest T-cell immune response- with construct expressing Omp16 (ELISPOT, 337 ± 52-651 ± 45 spots/4×105cells), which was comparable (P > 0.05) to the response induced by the commercial vaccine B. abortus 19. Interestingly, c. immunization appeared to be optimal for eliciting T-cell immune response. In guinea pigs, the highest protective efficacy after challenge with B. abortus 544 was achieved with Omp16 expressing constructs in both monovalent or bivalent vaccine formulations; protective efficacy was comparable to those induced by

  1. Functional assay of Salmonella typhi OmpC using reconstituted large unilamellar vesicles: a general method for characterization of outer membrane proteins.

    Science.gov (United States)

    Sundara Baalaji, N; Mathew, M K; Krishnaswamy, S

    2006-10-01

    The immunodominant trimeric beta-barrel outer membrane protein OmpC from Salmonella typhi, the causative agent of typhoid, has been functionally characterized here. The activity in the vesicle environment was studied in vitro using OmpC reconstituted into proteoliposomes. Passage of polysaccharides and polyethyleneglycols through OmpC has been examined to determine the permeability properties. The relative rate of neutral solute flux yields a radius of 1.1 nm for the S. typhi OmpC pore. This is almost double the pore size of Escherichia coli. This provides an example of large pore size present in the porins that form trimers as in the general bacterial porin family. The method used in this study provides a good membrane model for functional studies of porins.

  2. The BRITNeY Suite Animation Tool

    DEFF Research Database (Denmark)

    Westergaard, Michael; Lassen, Kristian Bisgaard

    2006-01-01

    This paper describes the BRITNeY suite, a tool which enables users to create visualizations of formal models. BRITNeY suite is integrated with CPN Tools, and we give an example of how to extend a simple stop-and-wait protocol with a visualization in the form of message sequence charts. We also sh...... examples of animations created during industrial projects to give an impression of what is possible with the BRITNeY suite....

  3. The Role of OmpR in the Expression of Genes of the KdgR Regulon Involved in the Uptake and Depolymerization of Oligogalacturonides in Yersinia enterocolitica

    Directory of Open Access Journals (Sweden)

    Marta Nieckarz

    2017-08-01

    Full Text Available Oligogalacturonide (OGA-specific porins of the KdgM family have previously been identified and characterized in enterobacterial plant pathogens. We found that deletion of the gene encoding response regulator OmpR causes the porin KdgM2 to become one of the most abundant proteins in the outer membrane of the human enteropathogen Yersinia enterocolitica. Reporter gene fusion and real-time PCR analysis confirmed that the expression of kdgM2 is repressed by OmpR. We also found that kdgM2 expression is subject to negative regulation by KdgR, a specific repressor of genes involved in the uptake and metabolism of pectin derivatives in plant pathogens. The additive effect of kdgR and ompR mutations suggested that KdgR and OmpR regulate kdgM2 expression independently. We confirmed that kdgM2 occurs in an operon with the pelP gene, encoding the periplasmic pectate lyase PelP. A pectinolytic assay showed strong upregulation of PelP production/activity in a Y. enterocolitica strain lacking OmpR and KdgR, which corroborates the repression exerted by these regulators on kdgM2. In addition, our data showed that OmpR is responsible for up regulation of the kdgM1 gene encoding the second specific oligogalacturonide porin KdgM1. This indicates the involvement of OmpR in the reciprocal regulation of both KdgM1 and KdgM2. Moreover, we demonstrated the negative impact of OmpR on kdgR transcription, which might positively affect the expression of genes of the KdgR regulon. Binding of OmpR to the promoter regions of the kdgM2-pelP-sghX operon, and kdgM1 and kdgR genes was confirmed using the electrophoretic mobility shift assay, suggesting that OmpR can directly regulate their transcription. We also found that the overexpression of porin KdgM2 increases outer membrane permeability. Thus, OmpR-mediated regulation of the KdgM porins may contribute to the fitness of Y. enterocolitica in particular local environments.

  4. HPC Benchmark Suite NMx, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Intelligent Automation Inc., (IAI) and University of Central Florida (UCF) propose to develop a comprehensive numerical test suite for benchmarking current and...

  5. ASDA - Advanced Suit Design Analyzer computer program

    Science.gov (United States)

    Bue, Grant C.; Conger, Bruce C.; Iovine, John V.; Chang, Chi-Min

    1992-01-01

    An ASDA model developed to evaluate the heat and mass transfer characteristics of advanced pressurized suit design concepts for low pressure or vacuum planetary applications is presented. The model is based on a generalized 3-layer suit that uses the Systems Integrated Numerical Differencing Analyzer '85 in conjunction with a 41-node FORTRAN routine. The latter simulates the transient heat transfer and respiratory processes of a human body in a suited environment. The user options for the suit encompass a liquid cooled garment, a removable jacket, a CO2/H2O permeable layer, and a phase change layer.

  6. Dual Regulation of the Small RNA MicC and the Quiescent Porin OmpN in Response to Antibiotic Stress in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Sushovan Dam

    2017-12-01

    Full Text Available Antibiotic resistant Gram-negative bacteria are a serious threat for public health. The permeation of antibiotics through their outer membrane is largely dependent on porin, changes in which cause reduced drug uptake and efficacy. Escherichia coli produces two major porins, OmpF and OmpC. MicF and MicC are small non-coding RNAs (sRNAs that modulate the expression of OmpF and OmpC, respectively. In this work, we investigated factors that lead to increased production of MicC. micC promoter region was fused to lacZ, and the reporter plasmid was transformed into E. coli MC4100 and derivative mutants. The response of micC–lacZ to antimicrobials was measured during growth over a 6 h time period. The data showed that the expression of micC was increased in the presence of β-lactam antibiotics and in an rpoE depleted mutant. Interestingly, the same conditions enhanced the activity of an ompN–lacZ fusion, suggesting a dual transcriptional regulation of micC and the quiescent adjacent ompN. Increased levels of OmpN in the presence of sub-inhibitory concentrations of chemicals could not be confirmed by Western blot analysis, except when analyzed in the absence of the sigma factor σE. We suggest that the MicC sRNA acts together with the σE envelope stress response pathway to control the OmpC/N levels in response to β-lactam antibiotics.

  7. Application of virtual phase-shifting speckle-interferometry for detection of polymorphism in the Chlamydia trachomatis omp1 gene

    Science.gov (United States)

    Feodorova, Valentina A.; Saltykov, Yury V.; Zaytsev, Sergey S.; Ulyanov, Sergey S.; Ulianova, Onega V.

    2018-04-01

    Method of phase-shifting speckle-interferometry has been used as a new tool with high potency for modern bioinformatics. Virtual phase-shifting speckle-interferometry has been applied for detection of polymorphism in the of Chlamydia trachomatis omp1 gene. It has been shown, that suggested method is very sensitive to natural genetic mutations as single nucleotide polymorphism (SNP). Effectiveness of proposed method has been compared with effectiveness of the newest bioinformatic tools, based on nucleotide sequence alignment.

  8. Long-term trends in invasive Haemophilus influenzae type B disease among indigenous Australian children following use of PRP-OMP and PRP-T vaccines.

    Science.gov (United States)

    Menzies, Robert Ian; Bremner, Kyla Margaret; Wang, Han; Beard, Frank Hudson; McIntyre, Peter Bruce

    2015-06-01

    Among indigenous populations with high incidence and early onset of invasive Haemophilus influenzae type b (Hib) disease, PRP-OMP vaccines are used in the United States and PRP-T vaccines in Canada. In Australia, PRP-OMP vaccines were exclusively used in indigenous children from 1993 until they were replaced by PRP-T between late 2005 and 2009. Analytic descriptive study of 20 years of enhanced surveillance data (1993-2013) for invasive Hib disease in Australian children PRP-OMP period (1993-1996) to 6.2 (95% CI: 4.0, 9.2) and 4.7 (95% CI: 1.7, 10.3) in the later PRP-OMP (1996-2009) and PRP-T periods (2009-2013), respectively. The indigenous:nonindigenous incidence rate ratio increased to 43 (95% CI: 16, 145) and 58 (95% CI: 7, 2660) in the later PRP-OMP and PRP-T periods, respectively, more than 10-fold higher than in lesser-incidence Australian regions. We found no change in Hib incidence among indigenous Australian children living in high-incidence regions in the first 4 years following a change to PRP-T-containing combination vaccines. This may be of relevance to North American indigenous populations characterized by suboptimal living conditions and young age of onset for whom PRP-OMP continues to be recommended, such as Alaska Natives.

  9. The Shigella flexneri OmpA amino acid residues 188EVQ190 are essential for the interaction with the virulence factor PhoN2.

    Science.gov (United States)

    Scribano, Daniela; Damico, Rosanna; Ambrosi, Cecilia; Superti, Fabiana; Marazzato, Massimiliano; Conte, Maria Pia; Longhi, Catia; Palamara, Anna Teresa; Zagaglia, Carlo; Nicoletti, Mauro

    2016-12-01

    Shigella flexneri is an intracellular pathogen that deploys an arsenal of virulence factors promoting host cell invasion, intracellular multiplication and intra- and inter-cellular dissemination. We have previously reported that the interaction between apyrase (PhoN2), a periplasmic ATP-diphosphohydrolase, and the C-terminal domain of the outer membrane (OM) protein OmpA is likely required for proper IcsA exposition at the old bacterial pole and thus for full virulence expression of Shigella flexneri (Scribano et al., 2014). OmpA, that is the major OM protein of Gram-negative bacteria, is a multifaceted protein that plays many different roles both in the OM structural integrity and in the virulence of several pathogens. Here, by using yeast two-hybrid technology and by constructing an in silico 3D model of OmpA from S. flexneri 5a strain M90T, we observed that the OmpA residues 188 EVQ 190 are likely essential for PhoN2-OmpA interaction. The 188 EVQ 190 amino acids are located within a flexible region of the OmpA protein that could represent a scaffold for protein-protein interaction.

  10. A comprehensive analysis of the Omp85/TpsB protein superfamily structural diversity, taxonomic occurrence, and evolution

    Science.gov (United States)

    Heinz, Eva; Lithgow, Trevor

    2014-01-01

    Members of the Omp85/TpsB protein superfamily are ubiquitously distributed in Gram-negative bacteria, and function in protein translocation (e.g., FhaC) or the assembly of outer membrane proteins (e.g., BamA). Several recent findings are suggestive of a further level of variation in the superfamily, including the identification of the novel membrane protein assembly factor TamA and protein translocase PlpD. To investigate the diversity and the causal evolutionary events, we undertook a comprehensive comparative sequence analysis of the Omp85/TpsB proteins. A total of 10 protein subfamilies were apparent, distinguished in their domain structure and sequence signatures. In addition to the proteins FhaC, BamA, and TamA, for which structural and functional information is available, are families of proteins with so far undescribed domain architectures linked to the Omp85 β-barrel domain. This study brings a classification structure to a dynamic protein superfamily of high interest given its essential function for Gram-negative bacteria as well as its diverse domain architecture, and we discuss several scenarios of putative functions of these so far undescribed proteins. PMID:25101071

  11. Sibelius. Karelia Suite, Op. 11 / Robert Layton

    Index Scriptorium Estoniae

    Layton, Robert

    1996-01-01

    Uuest heliplaadist "Sibelius. Karelia Suite, Op. 11. Luonnotar, Op. 70 a. Andante festivo. The Oceanides, Op. 73. King Christian II, Op. 27-Suite. Finlandia, Op. 26a. Gothenburg Symphony Orchester, Neeme Järvi" DG 447 760-2GH (72 minutes: DDD)

  12. Evaluating Suit Fit Using Performance Degradation

    Science.gov (United States)

    Margerum, Sarah E.; Cowley, Matthew; Harvill, Lauren; Benson, Elizabeth; Rajulu, Sudhakar

    2011-01-01

    The Mark III suit has multiple sizes of suit components (arm, leg, and gloves) as well as sizing inserts to tailor the fit of the suit to an individual. This study sought to determine a way to identify the point an ideal suit fit transforms into a bad fit and how to quantify this breakdown using mobility-based physical performance data. This study examined the changes in human physical performance via degradation of the elbow and wrist range of motion of the planetary suit prototype (Mark III) with respect to changes in sizing and as well as how to apply that knowledge to suit sizing options and improvements in suit fit. The methods implemented in this study focused on changes in elbow and wrist mobility due to incremental suit sizing modifications. This incremental sizing was within a range that included both optimum and poor fit. Suited range of motion data was collected using a motion analysis system for nine isolated and functional tasks encompassing the elbow and wrist joints. A total of four subjects were tested with motions involving both arms simultaneously as well as the right arm only. The results were then compared across sizing configurations. The results of this study indicate that range of motion may be used as a viable parameter to quantify at what stage suit sizing causes a detriment in performance; however the human performance decrement appeared to be based on the interaction of multiple joints along a limb, not a single joint angle. The study was able to identify a preliminary method to quantify the impact of size on performance and to develop a means to gauge tolerances around optimal size. More work is needed to improve the assessment of optimal fit and to compensate for multiple joint interactions.

  13. Evaluation of recombinant porin (rOmp2a) protein as a potential antigen candidate for serodiagnosis of Human Brucellosis.

    Science.gov (United States)

    Pathak, Prachi; Kumar, Ashu; Thavaselvam, Duraipandian

    2017-07-11

    Brucellosis is an important zoonotic disease caused by different Brucella species and human brucellosis is commonly prevalent in different states of India. Among various Brucella species, B. melitensis is most pathogenic to human and included as category B biothreat which can cause infection through aerosol, cut, wounds in skin and contact with infected animals. The diagnosis of human brucellosis is very important for proper treatment and management of disease as there is no vaccine available for human use. The present study was designed to clone, express and purify immunodominant recombinant omp2a (rOmp2a) porin protein of B. melitensis and to evaluate this new antigen candidate for specific serodiagnosis of human brucellosis by highly sensitive iELISA (indirect enzyme linked immunosorbent assay). Omp2a gene of B. melitensis 16 M strain was cloned and expressed in pET-SUMO expression system. The recombinant protein was purified under denaturing conditions using 8 M urea. The purified recombinant protein was confirmed by western blotting by reacting with anti-HIS antibody. The sero-reactivity of the recombinant protein was also checked by reacting with antisera of experimentally infected mice with B. melitensis 16 M at different time points. Serodiagnostic potential of recombinant porin antigen was tested against 185 clinical serum samples collected from regions endemic to brucellosis in southern part of India by iELISA. The samples were grouped into five groups. Group 1 contained cultured confirmed positive serum samples of brucellosis (n = 15), group 2 contained sera samples from positive cases of brucellosis previously tested by conventional methods of RBPT (n = 28) and STAT (n = 26), group 3 contained sera samples negative by RBPT(n = 36) and STAT (n = 32), group 4 contained sera samples of other febrile illness and PUO case (n = 35) and group 5 contained confirmed negative sera samples from healthy donors (n = 23). The rOmp2a was found to be

  14. A novel Geobacteraceae-specific outer membrane protein J (OmpJ is essential for electron transport to Fe (III and Mn (IV oxides in Geobacter sulfurreducens

    Directory of Open Access Journals (Sweden)

    Schiffer Marianne

    2005-07-01

    Full Text Available Abstract Background Metal reduction is thought to take place at or near the bacterial outer membrane and, thus, outer membrane proteins in the model dissimilatory metal-reducing organism Geobacter sulfurreducens are of interest to understand the mechanisms of Fe(III reduction in the Geobacter species that are the predominant Fe(III reducers in many environments. Previous studies have implicated periplasmic and outer membrane cytochromes in electron transfer to metals. Here we show that the most abundant outer membrane protein of G. sulfurreducens, OmpJ, is not a cytochrome yet it is required for metal respiration. Results When outer membrane proteins of G. sulfurreducens were separated via SDS-PAGE, one protein, designated OmpJ (outer membrane protein J, was particularly abundant. The encoding gene, which was identified from mass spectrometry analysis of peptide fragments, is present in other Geobacteraceae, but not in organisms outside this family. The predicted localization and structure of the OmpJ protein suggested that it was a porin. Deletion of the ompJ gene in G. sulfurreducens produced a strain that grew as well as the wild-type strain with fumarate as the electron acceptor but could not grow with metals, such as soluble or insoluble Fe (III and insoluble Mn (IV oxide, as the electron acceptor. The heme c content in the mutant strain was ca. 50% of the wild-type and there was a widespread loss of multiple cytochromes from soluble and membrane fractions. Transmission electron microscopy analyses of mutant cells revealed an unusually enlarged periplasm, which is likely to trigger extracytoplasmic stress response mechanisms leading to the degradation of periplasmic and/or outer membrane proteins, such as cytochromes, required for metal reduction. Thus, the loss of the capacity for extracellular electron transport in the mutant could be due to the missing c-type cytochromes, or some more direct, but as yet unknown, role of OmpJ in metal

  15. The OmpL37 surface-exposed protein is expressed by pathogenic Leptospira during infection and binds skin and vascular elastin.

    Science.gov (United States)

    Pinne, Marija; Choy, Henry A; Haake, David A

    2010-09-07

    Pathogenic Leptospira spp. shed in the urine of reservoir hosts into freshwater can be transmitted to a susceptible host through skin abrasions or mucous membranes causing leptospirosis. The infection process involves the ability of leptospires to adhere to cell surface and extracellular matrix components, a crucial step for dissemination and colonization of host tissues. Therefore, the elucidation of novel mediators of host-pathogen interaction is important in the discovery of virulence factors involved in the pathogenesis of leptospirosis. In this study, we assess the functional roles of transmembrane outer membrane proteins OmpL36 (LIC13166), OmpL37 (LIC12263), and OmpL47 (LIC13050), which we recently identified on the leptospiral surface. We determine the capacity of these proteins to bind to host tissue components by enzyme-linked immunosorbent assay. OmpL37 binds elastin preferentially, exhibiting dose-dependent, saturating binding to human skin (K(d), 104±19 nM) and aortic elastin (K(d), 152±27 nM). It also binds fibrinogen (K(d), 244±15 nM), fibrinogen fragment D (K(d), 132±30 nM), plasma fibronectin (K(d), 359±68 nM), and murine laminin (K(d), 410±81 nM). The binding to human skin elastin by both recombinant OmpL37 and live Leptospira interrogans is specifically enhanced by rabbit antiserum for OmpL37, suggesting the involvement of OmpL37 in leptospiral binding to elastin and also the possibility that host-generated antibodies may promote rather than inhibit the adherence of leptospires to elastin-rich tissues. Further, we demonstrate that OmpL37 is recognized by acute and convalescent leptospirosis patient sera and also by Leptospira-infected hamster sera. Finally, OmpL37 protein is detected in pathogenic Leptospira serovars and not in saprophytic Leptospira. Thus, OmpL37 is a novel elastin-binding protein of pathogenic Leptospira that may be promoting attachment of Leptospira to host tissues.

  16. Z-1 Prototype Space Suit Testing Summary

    Science.gov (United States)

    Ross, Amy

    2013-01-01

    The Advanced Space Suit team of the NASA-Johnson Space Center performed a series of test with the Z-1 prototype space suit in 2012. This paper discusses, at a summary level, the tests performed and results from those tests. The purpose of the tests were two-fold: 1) characterize the suit performance so that the data could be used in the downselection of components for the Z-2 Space Suit and 2) develop interfaces with the suitport and exploration vehicles through pressurized suit evaluations. Tests performed included isolated and functional range of motion data capture, Z-1 waist and hip testing, joint torque testing, CO2 washout testing, fit checks and subject familiarizations, an exploration vehicle aft deck and suitport controls interface evaluation, delta pressure suitport tests including pressurized suit don and doff, and gross mobility and suitport ingress and egress demonstrations in reduced gravity. Lessons learned specific to the Z-1 prototype and to suit testing techniques will be presented.

  17. Expression of the AcrAB Components of the AcrAB-TolC Multidrug Efflux Pump of Yersinia enterocolitica Is Subject to Dual Regulation by OmpR.

    Directory of Open Access Journals (Sweden)

    Adrianna Raczkowska

    Full Text Available OmpR is a transcriptional regulator implicated in the control of various cellular processes and functions in Enterobacteriaceae. This study was undertaken to identify genes comprising the OmpR regulon in the human gastrointestinal pathogen Yersinia enterocolitica. Derivatives of an ompR-negative strain with random transposon insertions creating transcriptional fusions with the reporter gene lacZ were isolated. These were supplied with the wild-type ompR allele in trans and then screened for OmpR-dependent changes in β-galactosidase activity. Using this strategy, five insertions in genes/operons positively regulated by OmpR and two insertions in genes negatively regulated by this protein were identified. Genetic analysis of one of these fusion strains revealed that the gene acrR, encoding transcriptional repressor AcrR is negatively regulated by OmpR. Differential analysis of membrane proteins by SDS-PAGE followed by mass spectrometry identified the protein AcrB, a component of the AcrAB-TolC multidrug efflux pump, as being positively regulated by OmpR. Analysis of the activity of the acrR and acrAB promoters using gfp fusions confirmed their OmpR-dependent repression and activation, respectively. The identification of putative OmpR-binding sites and electrophoretic mobility shift assays confirmed that this regulator binds specifically to both promoter regions with different affinity. Examination of the activity of the acrR and acrAB promoters after the exposure of cells to different chemicals showed that bile salts can act as an OmpR-independent inducer. Taken together, our findings suggest that OmpR positively controls the expression of the AcrAB-TolC efflux pump involved in the adaptive response of Y. enterocolitica O:9 to different chemical stressors, thus conferring an advantage in particular ecological niches.

  18. Oracle SOA Suite 11g performance cookbook

    CERN Document Server

    Brasier, Matthew; Wright, Nicholas

    2013-01-01

    This is a Cookbook with interesting, hands-on recipes, giving detailed descriptions and lots of practical walkthroughs for boosting the performance of your Oracle SOA Suite.This book is for Oracle SOA Suite 11g administrators, developers, and architects who want to understand how they can maximise the performance of their SOA Suite infrastructure. The recipes contain easy to follow step-by-step instructions and include many helpful and practical tips. It is suitable for anyone with basic operating system and application server administration experience.

  19. HPC Benchmark Suite NMx, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — In the phase II effort, Intelligent Automation Inc., (IAI) and University of Central Florida (UCF) propose to develop a comprehensive numerical test suite for...

  20. Coupled Human-Space Suit Mobility Studies

    Data.gov (United States)

    National Aeronautics and Space Administration — Current EVA mobility studies only allow for comparisons of how the suit moves when actuated by a human and how the human moves when unsuited. There are now new...

  1. Strauss: Der Rosenkavalier - Suite / Michael Kennedy

    Index Scriptorium Estoniae

    Kennedy, Michael

    1990-01-01

    Uuest heliplaadist "Strauss: Der Rosenkavalier - Suite, Salome-Dance of the seven veils, Capriccio-Prelude, Intermezzo, Morgen Mittag um elf! Felicity Lott, Scottish National Orchestra, Neeme Järvi" Chandos ABRD 1397. ABTD 1397. CHAN 8758

  2. Interoperative efficiency in minimally invasive surgery suites.

    Science.gov (United States)

    van Det, M J; Meijerink, W J H J; Hoff, C; Pierie, J P E N

    2009-10-01

    Performing minimally invasive surgery (MIS) in a conventional operating room (OR) requires additional specialized equipment otherwise stored outside the OR. Before the procedure, the OR team must collect, prepare, and connect the equipment, then take it away afterward. These extra tasks pose a thread to OR efficiency and may lengthen turnover times. The dedicated MIS suite has permanently installed laparoscopic equipment that is operational on demand. This study presents two experiments that quantify the superior efficiency of the MIS suite in the interoperative period. Preoperative setup and postoperative breakdown times in the conventional OR and the MIS suite in an experimental setting and in daily practice were analyzed. In the experimental setting, randomly chosen OR teams simulated the setup and breakdown for a standard laparoscopic cholecystectomy (LC) and a complex laparoscopic sigmoid resection (LS). In the clinical setting, the interoperative period for 66 LCs randomly assigned to the conventional OR or the MIS suite were analyzed. In the experimental setting, the setup and breakdown times were significantly shorter in the MIS suite. The difference between the two types of OR increased for the complex procedure: 2:41 min for the LC (p < 0.001) and 10:47 min for the LS (p < 0.001). In the clinical setting, the setup and breakdown times as a whole were not reduced in the MIS suite. Laparoscopic setup and breakdown times were significantly shorter in the MIS suite (mean difference, 5:39 min; p < 0.001). Efficiency during the interoperative period is significantly improved in the MIS suite. The OR nurses' tasks are relieved, which may reduce mental and physical workload and improve job satisfaction and patient safety. Due to simultaneous tasks of other disciplines, an overall turnover time reduction could not be achieved.

  3. Space Suit Joint Torque Measurement Method Validation

    Science.gov (United States)

    Valish, Dana; Eversley, Karina

    2012-01-01

    In 2009 and early 2010, a test method was developed and performed to quantify the torque required to manipulate joints in several existing operational and prototype space suits. This was done in an effort to develop joint torque requirements appropriate for a new Constellation Program space suit system. The same test method was levied on the Constellation space suit contractors to verify that their suit design met the requirements. However, because the original test was set up and conducted by a single test operator there was some question as to whether this method was repeatable enough to be considered a standard verification method for Constellation or other future development programs. In order to validate the method itself, a representative subset of the previous test was repeated, using the same information that would be available to space suit contractors, but set up and conducted by someone not familiar with the previous test. The resultant data was compared using graphical and statistical analysis; the results indicated a significant variance in values reported for a subset of the re-tested joints. Potential variables that could have affected the data were identified and a third round of testing was conducted in an attempt to eliminate and/or quantify the effects of these variables. The results of the third test effort will be used to determine whether or not the proposed joint torque methodology can be applied to future space suit development contracts.

  4. Oral immunization with recombinant Lactobacillus casei expressing OmpAI confers protection against Aeromonas veronii challenge in common carp, Cyprinus carpio.

    Science.gov (United States)

    Zhang, Dong-Xing; Kang, Yuan-Huan; Chen, Long; Siddiqui, Shahrood Ahmed; Wang, Chun-Feng; Qian, Ai-Dong; Shan, Xiao-Feng

    2018-01-01

    Aeromonas veronii is a gram-negative pathogen capable of infecting both fish and mammals, including humans, and natural infection in fish results in irreparable damage to the aquaculture industry. Lactic acid bacteria (LAB) have a number of properties that make them attractive candidates as delivery vehicles for presentation to the mucosa sites of compounds with pharmaceutical interest, in particular vaccines. In this study, we generated two recombinant Lactobacillus casei (surface-displayed or secretory) expressing the OmpAI of A.veronii and evaluated the effect on immune responses in fish model. A 1022 bp gene fragment of the 42 kDa OmpAI antigen of A.veronii was cloned into pPG-1 (surface-displayed) and pPG-2 (secretory) and electrotransformed into Lactobacillus casei CC16. The recombinant plasmid in L.casei could be stably inherited over 50 generations, and production of OmpAI protein had slight limited effects on cells growth. Treatment of common carp with the recombinant vaccine candidate stimulated high serum or skin mucus specific antibody titers and induced a higher lysozyme, ACP, SOD activity, while fish fed with Lc-pPG or PBS had no detectable immobilizing immune responses. Expression of IL-10, IL-β, IFN-γ, TNF-α genes in the group immunized with recombinant L.casei were significantly (P casei strains were directly delivered and survive throughout the intestinal tract, the recombinant OmpAI was also detected in intestine mucosal. The results showed that common carp received Lc-pPG1-OmpAI (66.7%) and Lc-pPG2-OmpAI (50.0%) had higher survival rates compared with the controls after challenge with A.veronii, indicating that Lc-pPG1-OmpAI and Lc-pPG2-OmpAI had beneficial effects on immune response and enhanced disease resistance of common carp against A.veronii infection. Our study here demonstrates, for the first time, the ability of recombinant L.casei as oral vaccine against A.veronii infection in carps. The combination of OmpAI delivery and LAB

  5. Insights into PG-binding, conformational change, and dimerization of the OmpA C-terminal domains from Salmonella enterica serovar Typhimurium and Borrelia burgdorferi: Characterization of OmpA C-Terminal Domain

    Energy Technology Data Exchange (ETDEWEB)

    Tan, Kemin [Center for Structural Genomics of Infectious Diseases, University of Chicago, 5735 South Ellis Avenue Chicago Illinois 60637; Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Structural Biology Center, Biosciences, Argonne National Laboratory, Argonne Illinois 60439; Deatherage Kaiser, Brooke L. [National Security Directorate, Pacific Northwest National Laboratory, Richland Washington 99352; Wu, Ruiying [Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Cuff, Marianne [Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Fan, Yao [Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Bigelow, Lance [Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Jedrzejczak, Robert P. [Center for Structural Genomics of Infectious Diseases, University of Chicago, 5735 South Ellis Avenue Chicago Illinois 60637; Adkins, Joshua N. [Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland Washington 99352; Cort, John R. [Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland Washington 99352; Babnigg, Gyorgy [Center for Structural Genomics of Infectious Diseases, University of Chicago, 5735 South Ellis Avenue Chicago Illinois 60637; Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Joachimiak, Andrzej [Center for Structural Genomics of Infectious Diseases, University of Chicago, 5735 South Ellis Avenue Chicago Illinois 60637; Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Structural Biology Center, Biosciences, Argonne National Laboratory, Argonne Illinois 60439

    2017-06-19

    S. Typhimurium can induce both humoral and cell-mediated responses when establishing itself in the host. These responses are primarily stimulated against the lipopolysaccharide and major outer membrane (OM) proteins of the bacterium. OmpA is one of these major OM proteins. It comprises a N-terminal eight-stranded -barrel membrane domain and a C-terminal so-called OmpA C-terminal domain (OmpACTD). The OmpACTD and its homologs are believed to bind to peptidoglycan (PG) within the periplasm, maintaining bacterial osmotic homeostasis and modulating the permeability and integrity of the outer membrane. Here we present the structures of two forms of the OmpACTD of S. Typhimurium (STOmpACTD) and one structure of the less-studied OmpACTD of Borrelia burgdorferi (BbOmpACTD). In the open form of STOmpACTD, an aspartic acid residue from a long 2-3 loop points into the binding pocket, suggesting that an anion group such as a carboxylate group from PG is favored at the binding site. In the closed form of STOmpACTD and in the structure of BbOmpACTD, a sulfate group from the crystallization buffer is tightly bound at the equivalent site. The differences between the closed and open forms of STOmpACTD, suggest a large conformational change that includes an extension of 3 helix by ordering a part of 2-3 loop. We suggest that the sulfate anion observed in these structures mimics the carboxylate group of PG when bound to STOmpACTD. In addition, the binding of PG or a ligand mimic may enhance dimerization of STOmpACTD, or possibly that of full length STOmpA.

  6. Identification of OmpR-family response regulators interacting with thioredoxin in the Cyanobacterium Synechocystis sp. PCC 6803.

    Directory of Open Access Journals (Sweden)

    Taro Kadowaki

    Full Text Available The redox state of the photosynthetic electron transport chain is known to act as a signal to regulate the transcription of key genes involved in the acclimation responses to environmental changes. We hypothesized that the protein thioredoxin (Trx acts as a mediator connecting the redox state of the photosynthetic electron transport chain and transcriptional regulation, and established a screening system to identify transcription factors (TFs that interact with Trx. His-tagged TFs and S-tagged mutated form of Trx, TrxMC35S, whose active site cysteine 35 was substituted with serine to trap the target interacting protein, were co-expressed in E. coli cells and Trx-TF complexes were detected by immuno-blotting analysis. We examined the interaction between Trx and ten OmpR family TFs encoded in the chromosome of the cyanobacterium Synechocystis sp. PCC 6803 (S.6803. Although there is a highly conserved cysteine residue in the receiver domain of all OmpR family TFs, only three, RpaA (Slr0115, RpaB (Slr0947 and ManR (Slr1837, were identified as putative Trx targets [corrected].The recombinant forms of wild-type TrxM, RpaA, RpaB and ManR proteins from S.6803 were purified following over-expression in E. coli and their interaction was further assessed by monitoring changes in the number of cysteine residues with free thiol groups. An increase in the number of free thiols was observed after incubation of the oxidized TFs with Trx, indicating the reduction of cysteine residues as a consequence of interaction with Trx. Our results suggest, for the first time, the possible regulation of OmpR family TFs through the supply of reducing equivalents from Trx, as well as through the phospho-transfer from its cognate sensor histidine kinase.

  7. Suites of dwarfs around Nearby giant galaxies

    International Nuclear Information System (INIS)

    Karachentsev, Igor D.; Kaisina, Elena I.; Makarov, Dmitry I.

    2014-01-01

    The Updated Nearby Galaxy Catalog (UNGC) contains the most comprehensive summary of distances, radial velocities, and luminosities for 800 galaxies located within 11 Mpc from us. The high density of observables in the UNGC makes this sample indispensable for checking results of N-body simulations of cosmic structures on a ∼1 Mpc scale. The environment of each galaxy in the UNGC was characterized by a tidal index Θ 1 , depending on the separation and mass of the galaxy's main disturber (MD). We grouped UNGC galaxies with a common MD in suites, and ranked suite members according to their Θ 1 . All suite members with positive Θ 1 are assumed to be physical companions of the MD. About 58% of the sample are members of physical groups. The distribution of suites by the number of members, n, follows a relation N(n) ∼ n –2 . The 20 most populated suites contain 468 galaxies, i.e., 59% of the UNGC sample. The fraction of MDs among the brightest galaxies is almost 100% and drops to 50% at M B = –18 m . We discuss various properties of MDs, as well as galaxies belonging to their suites. The suite abundance practically does not depend on the morphological type, linear diameter, or hydrogen mass of the MD, the tightest correlation being with the MD dynamical mass. Dwarf galaxies around MDs exhibit well-known segregation effects: the population of the outskirts has later morphological types, richer H I contents, and higher rates of star formation activity. Nevertheless, there are some intriguing cases where dwarf spheroidal galaxies occur at the far periphery of the suites, as well as some late-type dwarfs residing close to MDs. Comparing simulation results with galaxy groups, most studies assume the Local Group is fairly typical. However, we recognize that the nearby groups significantly differ from each other and there is considerable variation in their properties. The suites of companions around the Milky Way and M31, consisting of the Local Group, do not

  8. Analytical Tools for Space Suit Design

    Science.gov (United States)

    Aitchison, Lindsay

    2011-01-01

    As indicated by the implementation of multiple small project teams within the agency, NASA is adopting a lean approach to hardware development that emphasizes quick product realization and rapid response to shifting program and agency goals. Over the past two decades, space suit design has been evolutionary in approach with emphasis on building prototypes then testing with the largest practical range of subjects possible. The results of these efforts show continuous improvement but make scaled design and performance predictions almost impossible with limited budgets and little time. Thus, in an effort to start changing the way NASA approaches space suit design and analysis, the Advanced Space Suit group has initiated the development of an integrated design and analysis tool. It is a multi-year-if not decadal-development effort that, when fully implemented, is envisioned to generate analysis of any given space suit architecture or, conversely, predictions of ideal space suit architectures given specific mission parameters. The master tool will exchange information to and from a set of five sub-tool groups in order to generate the desired output. The basic functions of each sub-tool group, the initial relationships between the sub-tools, and a comparison to state of the art software and tools are discussed.

  9. Micro-coil NMR to monitor optimization of the reconstitution conditions for the integral membrane protein OmpW in detergent micelles

    International Nuclear Information System (INIS)

    Stanczak, Pawel; Zhang Qinghai; Horst, Reto; Serrano, Pedro; Wüthrich, Kurt

    2012-01-01

    Optimization of aqueous solutions of the integral membrane protein (IMP) OmpW for NMR structure determination has been monitored with micro-coil NMR, which enables the acquisition of NMR spectra using only micrograms of protein and detergent. The detergent 30-Fos (2-undecylphosphocholine) was found to yield the best 2D [ 15 N, 1 H]-TROSY correlation NMR spectra of [ 2 H, 15 N]-labeled OmpW. For the OmpW structure determination we then optimized the 30-Fos concentration, the sample temperature and long-time stability, and the deuteration level of the protein. Some emerging guidelines for reconstitution of β-barrel integral membrane proteins in structural biology are discussed.

  10. Z-2 Prototype Space Suit Development

    Science.gov (United States)

    Ross, Amy; Rhodes, Richard; Graziosi, David; Jones, Bobby; Lee, Ryan; Haque, Bazle Z.; Gillespie, John W., Jr.

    2014-01-01

    NASA's Z-2 prototype space suit is the highest fidelity pressure garment from both hardware and systems design perspectives since the Space Shuttle Extravehicular Mobility Unit (EMU) was developed in the late 1970's. Upon completion the Z-2 will be tested in the 11 foot human-rated vacuum chamber and the Neutral Buoyancy Laboratory (NBL) at the NASA Johnson Space Center to assess the design and to determine applicability of the configuration to micro-, low- (asteroid), and planetary- (surface) gravity missions. This paper discusses the 'firsts' that the Z-2 represents. For example, the Z-2 sizes to the smallest suit scye bearing plane distance for at least the last 25 years and is being designed with the most intensive use of human models with the suit model.

  11. Advanced EVA Suit Camera System Development Project

    Science.gov (United States)

    Mock, Kyla

    2016-01-01

    The National Aeronautics and Space Administration (NASA) at the Johnson Space Center (JSC) is developing a new extra-vehicular activity (EVA) suit known as the Advanced EVA Z2 Suit. All of the improvements to the EVA Suit provide the opportunity to update the technology of the video imagery. My summer internship project involved improving the video streaming capabilities of the cameras that will be used on the Z2 Suit for data acquisition. To accomplish this, I familiarized myself with the architecture of the camera that is currently being tested to be able to make improvements on the design. Because there is a lot of benefit to saving space, power, and weight on the EVA suit, my job was to use Altium Design to start designing a much smaller and simplified interface board for the camera's microprocessor and external components. This involved checking datasheets of various components and checking signal connections to ensure that this architecture could be used for both the Z2 suit and potentially other future projects. The Orion spacecraft is a specific project that may benefit from this condensed camera interface design. The camera's physical placement on the suit also needed to be determined and tested so that image resolution can be maximized. Many of the options of the camera placement may be tested along with other future suit testing. There are multiple teams that work on different parts of the suit, so the camera's placement could directly affect their research or design. For this reason, a big part of my project was initiating contact with other branches and setting up multiple meetings to learn more about the pros and cons of the potential camera placements we are analyzing. Collaboration with the multiple teams working on the Advanced EVA Z2 Suit is absolutely necessary and these comparisons will be used as further progress is made for the overall suit design. This prototype will not be finished in time for the scheduled Z2 Suit testing, so my time was

  12. Distribution of the ompA-types among ruminant and swine pneumonic strains of Pasteurella multocida exhibiting various cap-locus and toxA patterns.

    Science.gov (United States)

    Vougidou, C; Sandalakis, V; Psaroulaki, A; Siarkou, V; Petridou, E; Ekateriniadou, L

    2015-05-01

    Pasteurella multocida is an important pathogen in food-producing animals and numerous virulence genes have been identified in an attempt to elucidate the pathogenesis of pasteurellosis. Currently, some of these genes including the capsule biosynthesis genes, the toxA and the OMPs-encoding genes have been suggested as epidemiological markers. However, the number of studies concerning ruminant isolates is limited, while, no attempt has ever been made to investigate the existence of ompA sequence diversity among P. multocida isolates. The aim of the present study was the comparative analysis of 144 P. multocida pneumonic isolates obtained from sheep, goats, cattle and pigs by determining the distribution of the ompA-types in conjunction with the cap-locus and toxA patterns. The ompA genotypes of the isolates were determined using both a PCR-RFLP method and DNA sequence analysis. The most prevalent capsule biosynthesis gene among the isolates was capA (86.1%); a noticeable, however, rate of capD-positive isolates (38.6%) was found among the ovine isolates that had been associated primarily with the capsule type A in the past. Moreover, an unexpectedly high percentage of toxA-positive pneumonic isolates was noticed among small ruminants (93.2% and 85.7% in sheep and goats, respectively), indicating an important epidemiological role of toxigenic P. multocida for these species. Despite their great heterogeneity, certain ompA-genotypes were associated with specific host species, showing evidence of a host preference. The OmpA-based PCR-RFLP method developed proved to be a valuable tool in typing P. multocida strains. Copyright © 2015 Elsevier GmbH. All rights reserved.

  13. Immunogenicity in chickens with orally administered recombinant chicken-borne Lactobacillus saerimneri expressing FimA and OmpC antigen of O78 avian pathogenic Escherichia coli.

    Science.gov (United States)

    Ma, Sun-Ting; Ding, Guo-Jie; Huang, Xue-Wei; Wang, Zi-Wei; Wang, Li; Yu, Mei-Ling; Shi, Wen; Jiang, Yan-Ping; Tang, Li-Jie; Xu, Yi-Gang; Li, Yi-Jing

    2018-03-01

    Avian colibacillosis is responsible for economic losses to poultry producers worldwide. To combat this, we aimed to develop an effective oral vaccine for chicken against O78 avian pathogenic Escherichia coli (APEC) infection through a Lactobacillus delivery system. Eight Lactobacillus strains isolated from the intestines of broiler chickens were evaluated based on their in vitro adherence ability to assess their potential as a delivery vector. Fimbrial subunit A (FimA) and outer-membrane protein C (OmpC) of APEC with and without fusion to dendritic cell-targeting peptide (DCpep) and microfold cell-targeting peptide (Co1) were displayed on the surface of Lactobacillus saerimneri M-11 and yielded vaccine groups (pPG-ompC-fimA/M-11 and pPG-ompC-fimA-Co1-DCpep/M-11, respectively). The colonization of the recombinant strains in vivo was assessed and the immunogenicity and protective efficacy of orally administered recombinant strains in chickens were evaluated. The colonization of the recombinant strains in vivo revealed no significant differences between the recombinant and wild-type strains. Chickens orally administered with vaccine groups showed significantly higher levels of OmpC/FimA-specific IgG in serum and mucosal IgA in cecum lavage, nasal lavage and stool compared to the pPG/M-11 group. After challenge with APEC CVCC1553, better protective efficacy was observed in chickens orally immunized with pPG-ompC-fimA/M-11 and pPG-ompC-fimA-Co1-DCpep/M-11, but no significant differences were observed between the two groups. Recombinant chicken-borne L. saerimneri M-11 showed good immunogenicity in chickens, suggesting that it may be a promising vaccine candidate against APEC infections. However, the activity of mammalian DCpep and Co1 was not significant in chickens.

  14. Immersion Suit Usage Within the RAAF

    Science.gov (United States)

    1992-01-01

    IMMERSION SUIT USED UVIC QDIS HOLDINGS 202. in 12 Sizes, held by ALSS 492SQN REQUIREMENTS No comment USAGE POLICY REFERENCE DIRAF) AAP 7215.004-1 (P3C...held by ALSS 492SQN. REQUIREMENTS No comment ISACE POLICY REFERENCE DIIAF) AAP 7215.004-1 (P3C Flight Manual) RAAF Supplement No 92 USAGE POUICY UVIC...TYPE P3C REFERENCE Telecon FLTLT Toft I I SQNfRESO AVMED Dated 22 Mar 91 IMMERSION SUIT USED UVIC QDIS HOLDINGS No comment REQUIREMENTS No comment USAGE

  15. Advanced Sensor Platform to Evaluate Manloads for Exploration Suit Architectures

    Data.gov (United States)

    National Aeronautics and Space Administration — Space suit manloads are defined as the outer bounds of force that the human occupant of a suit is able to exert onto the suit during motion. They are defined on a...

  16. What's New with MS Office Suites

    Science.gov (United States)

    Goldsborough, Reid

    2012-01-01

    If one buys a new PC, laptop, or netbook computer today, it probably comes preloaded with Microsoft Office 2010 Starter Edition. This is a significantly limited, advertising-laden version of Microsoft's suite of productivity programs, Microsoft Office. This continues the trend of PC makers providing ever more crippled versions of Microsoft's…

  17. Antigravity Suits For Studies Of Weightlessness

    Science.gov (United States)

    Kravik, Stein E.; Greenleaf, John

    1992-01-01

    Report presents results of research on use of "antigravity" suit, one applying positive pressure to lower body to simulate some effects of microgravity. Research suggests lower-body positive pressure is alternative to bed rest or immersion in water in terrestrial studies of cardioregulatory, renal, electrolyte, and hormonal changes induced in humans by microgravity.

  18. Prokofiev. "Romeo and Juliet" - Suites / Iran March

    Index Scriptorium Estoniae

    March, Iran

    1991-01-01

    Uuest heliplaadist "Prokofiev. "Romeo and Juliet" - Suites: N 1 Op. 64 bis a; N 2 Op. 64 ter b; N 3 Op. 101 c. Royal Scottish National Orchestra /Neeme Järvi" Chandos cassette ABTD 1536; CD CHAN 8940 (78 minutes) etc

  19. A Suite of Tools for Technology Assessment

    Science.gov (United States)

    2007-09-01

    Saden, Povinelli & Rosen, 1989). • This was a significant change in emphasis on the part of NASA, where technology had previously viewed as merely...Cost Analysis Symposium, April 13, 2005. A Suite of Tools for Technology Assessment 24 Bibliography - continued: • Sadin, Stanley T.; Povinelli

  20. 28 CFR 36.501 - Private suits.

    Science.gov (United States)

    2010-07-01

    ... ACCOMMODATIONS AND IN COMMERCIAL FACILITIES Enforcement § 36.501 Private suits. (a) General. Any person who is... order. Upon timely application, the court may, in its discretion, permit the Attorney General to... general public importance. Upon application by the complainant and in such circumstances as the court may...

  1. Open architecture of smart sensor suites

    Science.gov (United States)

    Müller, Wilmuth; Kuwertz, Achim; Grönwall, Christina; Petersson, Henrik; Dekker, Rob; Reinert, Frank; Ditzel, Maarten

    2017-10-01

    Experiences from recent conflicts show the strong need for smart sensor suites comprising different multi-spectral imaging sensors as core elements as well as additional non-imaging sensors. Smart sensor suites should be part of a smart sensor network - a network of sensors, databases, evaluation stations and user terminals. Its goal is to optimize the use of various information sources for military operations such as situation assessment, intelligence, surveillance, reconnaissance, target recognition and tracking. Such a smart sensor network will enable commanders to achieve higher levels of situational awareness. Within the study at hand, an open system architecture was developed in order to increase the efficiency of sensor suites. The open system architecture for smart sensor suites, based on a system-of-systems approach, enables combining different sensors in multiple physical configurations, such as distributed sensors, co-located sensors combined in a single package, tower-mounted sensors, sensors integrated in a mobile platform, and trigger sensors. The architecture was derived from a set of system requirements and relevant scenarios. Its mode of operation is adaptable to a series of scenarios with respect to relevant objects of interest, activities to be observed, available transmission bandwidth, etc. The presented open architecture is designed in accordance with the NATO Architecture Framework (NAF). The architecture allows smart sensor suites to be part of a surveillance network, linked e.g. to a sensor planning system and a C4ISR center, and to be used in combination with future RPAS (Remotely Piloted Aircraft Systems) for supporting a more flexible dynamic configuration of RPAS payloads.

  2. ANALYSIS OF DESIGN ELEMENTS IN SKI SUITS

    Directory of Open Access Journals (Sweden)

    Birsen Çileroğlu

    2014-06-01

    Full Text Available Popularity of Ski Sport in 19th century necessitated a new perspective on protective skiing clothing ag ainst the mountain climates and excessive cold. Winter clothing were the basis of ski attire during this period. By the beginning of 20th century lining cloth were used to minimize the wind effect. The difference between the men and women’s ski attire of the time consisted of a knee - length skirts worn over the golf trousers. Subsequent to the First World War, skiing suit models were influenced by the period uniforms and the producers reflected the fashion trends to the ski clothing. In conformance with th e prevailing trends, ski trousers were designed and produced for the women thus leading to reduction in gender differences. Increases in the ski tourism and holding of the first winter olympics in 1924 resulted in variations in ski attires, development of design characteristics, growth in user numbers, and enlargement of production capacities. Designers emphasized in their collections combined presence of elegance and practicality in the skiing attire. In 1930s, the ski suits influenced by pilots’ uniforms included characteristics permitting freedom of motion, and the design elements exhibited changes in terms of style, material and aerodynamics. In time, the ski attires showed varying design features distinguishing professionals from the amateurs. While protective functionality was primary consideration for the amateurs, for professionals the aerodynamic design was also a leading factor. Eventually, the increased differences in design characteristics were exhibited in ski suit collections, World reknown brands were formed, production and sales volumes showed significant rise. During 20th century the ski suits influenced by fashion trends to acquire unique styles reached a position of dominance to impact current fashion trends, and apart from sports attir es they became a style determinant in the clothing of cold climates. Ski suits

  3. Prevalence of Chlamydia trachomatis Genotypes in Men Who Have Sex with Men and Men Who Have Sex with Women Using Multilocus VNTR Analysis-ompA Typing in Guangzhou, China.

    Directory of Open Access Journals (Sweden)

    Xiaolin Qin

    Full Text Available Chlamydia trachomatis is one of the most prevalent bacterial sexually transmitted infection in China. Although C. trachomatis genotypes can be discriminated by outer membrane protein gene (ompA sequencing, currently available methods have limited resolutions. This study used a high-resolution genotyping method, namely, multilocus variable number tandem-repeat analysis with ompA sequencing (MLVA-ompA, to investigate the local epidemiology of C. trachomatis infections among men who have sex with men (MSM and men who have sex with women (MSW attending a sexually transmitted diseases (STD clinic in Guangzhou, China.Rectal specimens from MSM and urethral specimens from MSW were collected between January 2013 and July 2014 at the Guangdong Provincial Center STD clinic. The specimens were sent to the laboratory for analyses. All specimens that were tested positive for C. trachomatis by the commercial nucleic acid amplification tests were genotyped by MLVA-ompA.Fifty-one rectal specimens from MSM and 96 urethral specimens from MSW were identified with C. trachomatis. One hundred and forty-four of the 147 specimens were fully genotyped by MLVA-ompA. Rectal specimens from MSM were divided into four ompA genotypes and urethral specimens from MSW into nine genotypes. No mixed infections were found among all specimens. The most frequent genotypes were D, G, J, E and F. All specimens were further divided into 46 types after ompA genotyping was combined with MLVA. Genotypes D-8.7.1 and G-3.4a.3 were the most frequent among MSM, whereas genotypes D-3.4a.4, E-8.5.1, F-8.5.1, and J-3.4a.2 were the most frequent subtypes among MSW. The discriminatory index D was 0.90 for MLVA, 0.85 for ompA, and 0.95 for MLVA-ompA.The most prevalent MLVA-ompA genotypes were significantly different between MSM and MSW from Guangzhou, China. Moreover, MLVA-ompA represented a more favorable degree of discrimination than ompA and could be a reliable complement for ompA for the routine

  4. Host adaptation of Chlamydia pecorum towards low virulence evident in co-evolution of the ompA, incA, and ORF663 Loci.

    Science.gov (United States)

    Mohamad, Khalil Yousef; Kaltenboeck, Bernhard; Rahman, Kh Shamsur; Magnino, Simone; Sachse, Konrad; Rodolakis, Annie

    2014-01-01

    Chlamydia (C.) pecorum, an obligate intracellular bacterium, may cause severe diseases in ruminants, swine and koalas, although asymptomatic infections are the norm. Recently, we identified genetic polymorphisms in the ompA, incA and ORF663 genes that potentially differentiate between high-virulence C. pecorum isolates from diseased animals and low-virulence isolates from asymptomatic animals. Here, we expand these findings by including additional ruminant, swine, and koala strains. Coding tandem repeats (CTRs) at the incA locus encoded a variable number of repeats of APA or AGA amino acid motifs. Addition of any non-APA/AGA repeat motif, such as APEVPA, APAVPA, APE, or APAPE, associated with low virulence (PincA CTRs (P = 0.0028). In ORF663, high numbers of 15-mer CTRs correlated with low virulence (P = 0.0001). Correction for ompA phylogram position in ORF663 and incA abolished the correlation between genetic changes and virulence, demonstrating co-evolution of ompA, incA, and ORF663 towards low virulence. Pairwise divergence of ompA, incA, and ORF663 among isolates from healthy animals was significantly higher than among strains isolated from diseased animals (P≤10-5), confirming the longer evolutionary path traversed by low-virulence strains. All three markers combined identified 43 unique strains and 4 pairs of identical strains among all 57 isolates tested, demonstrating the suitability of these markers for epidemiological investigations.

  5. 16S rRNA and Omp31 Gene Based Molecular Characterization of Field Strains of B. melitensis from Aborted Foetus of Goats in India

    Science.gov (United States)

    Singh, Ajay; Gupta, Vivek Kumar; Kumar, Amit; Singh, Vikas Kumar; Nayakwadi, Shivasharanappa

    2013-01-01

    Brucellosis is a reemerging infectious zoonotic disease of worldwide importance. In human, it is mainly caused by Brucella melitensis, a natural pathogen for goats. In India, a large number of goats are reared in semi-intensive to intensive system within the close vicinity of human being. At present, there is no vaccination and control strategy for caprine brucellosis in the country. Thus, to formulate an effective control strategy, the status of etiological agent is essential. To cope up with these, the present study was conducted to isolate and identify the prevalent Brucella species in caprine brucellosis in India. The 30 samples (fetal membrane, fetal stomach content and vaginal swabs) collected throughout India from the aborted fetus of goats revealed the isolation of 05 isolates all belonging to Brucella melitensis biovars 3. All the isolates produced amplification products of 1412 and 720 bp in polymerase chain reaction with genus and species specific 16S rRNA and omp31 gene based primers, respectively. Moreover, the amplification of omp31 gene in all the isolates confirmed the presence of immuno dominant outer membrane protein (31 kDa omp) in all the field isolates of B. melitensis in aborted foetus of goats in India. These findings can support the development of omp31 based specific serodiagnostic test as well as vaccine for the control of caprine brucellosis in India. PMID:24453799

  6. Construction of a recombinant Lactococcus lactis strain expressing a fusion protein of Omp22 and HpaA from Helicobacter pylori for oral vaccine development.

    Science.gov (United States)

    Zhang, Rongguang; Duan, Guangcai; Shi, Qingfeng; Chen, Shuaiyin; Fan, Qingtang; Sun, Nan; Xi, Yuanlin

    2016-11-01

    To develop orally administrated anti-Helicobacter pylori vaccination, a Lactococcus lactis strain was genetically constructed for fusion expression of H. pylori protective antigens HpaA and Omp22. The fusion gene of omp22 and hpaA with an adapter encoding three glycines was cloned from a plasmid pMAL-c2x-omp22-hpaA into Escherichia coli MC1061 and L. lactis NZ3900 successively using a shutter vector pNZ8110. Expression of the fusion gene in L. lactis was induced with nisin resulting in production of proteins with molecular weights of 50 and 28 kDa. Both of them were immunoreactive with mouse anti-H. pylori sera as determined via western blotting. Oral vaccination of BALB/c mice using the L. lactis strain carrying pNZ8110-omp22-hpaA elicited significant systematic humoral immune response (P lactis with immunogenicity. This is a considerable step towards H. pylori vaccines.

  7. Extending and Enhancing SAS (Static Analysis Suite)

    CERN Document Server

    Ho, David

    2016-01-01

    The Static Analysis Suite (SAS) is an open-source software package used to perform static analysis on C and C++ code, helping to ensure safety, readability and maintainability. In this Summer Student project, SAS was enhanced to improve ease of use and user customisation. A straightforward method of integrating static analysis into a project at compilation time was provided using the automated build tool CMake. The process of adding checkers to the suite was streamlined and simplied by developing an automatic code generator. To make SAS more suitable for continuous integration, a reporting mechanism summarising results was added. This suitability has been demonstrated by inclusion of SAS in the Future Circular Collider Software nightly build system. Scalability of the improved package was demonstrated by using the tool to analyse the ROOT code base.

  8. Enhancements to the opera-3d suite

    International Nuclear Information System (INIS)

    Riley, C.P.

    1997-01-01

    The OPERA-3D suite of programs has been enhanced to include 2 additional 3 dimensional finite element based solvers, with complimentary features in the pre- and postprocessing. SOPRANO computes electromagnetic fields at high frequency including displacement current effects. It has 2 modules emdash a deterministic solution at a user defined frequency and an eigenvalue solution for modal analysis. It is suitable for designing microwave structures and cavities found in particle accelerators. SCALA computes electrostatic fields in the presence of space charge from charged particle beams. The user may define the emission characteristics of electrodes or plasma surfaces and compute the resultant space charge limited beams, including the presence of magnetic fields. Typical applications in particle accelerators are electron guns and ion sources. Other enhancements to the suite include additional capabilities in TOSCA and ELEKTRA, the static and dynamic solvers. copyright 1997 American Institute of Physics

  9. The BTeV Software Tutorial Suite

    International Nuclear Information System (INIS)

    Kutschke, Robert K.

    2004-01-01

    The BTeV Collaboration is starting to develop its C++ based offline software suite, an integral part of which is a series of tutorials. These tutorials are targeted at a diverse audience, including new graduate students, experienced physicists with little or no C++ experience, those with just enough C++ to be dangerous, and experts who need only an overview of the available tools. The tutorials must both teach C++ in general and the BTeV specific tools in particular. Finally, they must teach physicists how to find and use the detailed documentation. This report will review the status of the BTeV experiment, give an overview of the plans for and the state of the software and will then describe the plans for the tutorial suite

  10. A new device for the inflation of the antigravity suit.

    Science.gov (United States)

    Brodrick, P M

    1986-02-01

    The 'Schuco' orthopaedic tourniquet inflator can be simply converted into a suitable device for inflating an antigravity suit (G-suit). The antigravity suit may be used on neurosurgical patients undergoing procedures in the sitting position to help prevent hypotension and air embolism. The availability of this device may encourage the more widespread use of an antigravity suit in neuro-anaesthetic practice.

  11. MzrA-EnvZ Interactions in the Periplasm Influence the EnvZ/OmpR Two-Component Regulon▿ †

    Science.gov (United States)

    Gerken, Henri; Misra, Rajeev

    2010-01-01

    MzrA was identified as a modulator of the EnvZ/OmpR two-component signal transduction system. Previous evidence indicated that MzrA interacts with EnvZ and modulates its enzymatic activities to influence OmpR phosphate (OmpR∼P) levels. Moreover, MzrA was shown to connect the bacterial envelope stress response systems CpxA/CpxR and σE to EnvZ/OmpR to widen the defensive response regulatory network. In this study, experiments were carried out to establish whether the membrane or periplasmic domain of MzrA is critical for MzrA-EnvZ interactions and to reveal MzrA residues that play an important role in these interactions. Data obtained from chimeric constructs, in which the transmembrane domain of MzrA was replaced with the unrelated transmembrane domain of NarX or signal sequence of PhoA, showed that the transmembrane domain residues of MzrA do not play a critical role in MzrA-EnvZ interactions. The importance of the periplasmic domain of MzrA in MzrA-EnvZ interactions was revealed by characterizing bifunctional, fully soluble, and periplasmically localized MalE::MzrA chimeras. This was further corroborated through the isolation of loss-of-function, single-amino-acid substitutions in the conserved periplasmic domain of MzrA that interfered with MzrA-EnvZ binding in a bacterial two-hybrid system. Together, the data suggest that the binding of MzrA to EnvZ influences the ability of EnvZ to receive and/or respond to environmental signals in the periplasm and modulate its biochemical output to OmpR. PMID:20889743

  12. Space suit bioenergetics: framework and analysis of unsuited and suited activity.

    Science.gov (United States)

    Carr, Christopher E; Newman, Dava J

    2007-11-01

    Metabolic costs limit the duration and intensity of extravehicular activity (EVA), an essential component of future human missions to the Moon and Mars. Energetics Framework: We present a framework for comparison of energetics data across and between studies. This framework, applied to locomotion, differentiates between muscle efficiency and energy recovery, two concepts often confused in the literature. The human run-walk transition in Earth gravity occurs at the point for which energy recovery is approximately the same for walking and running, suggesting a possible role for recovery in gait transitions. Muscular Energetics: Muscle physiology limits the overall efficiency by which chemical energy is converted through metabolism to useful work. Unsuited Locomotion: Walking and running use different methods of energy storage and release. These differences contribute to the relative changes in the metabolic cost of walking and running as gravity is varied, with the metabolic cost of locomoting at a given velocity changing in proportion to gravity for running and less than in proportion for walking. Space Suits: Major factors affecting the energetic cost of suited movement include suit pressurization, gravity, velocity, surface slope, and space suit configuration. Apollo lunar surface EVA traverse metabolic rates, while unexpectedly low, were higher than other activity categories. The Lunar Roving Vehicle facilitated even lower metabolic rates, thus longer duration EVAs. Muscles and tendons act like springs during running; similarly, longitudinal pressure forces in gas pressure space suits allow spring-like storage and release of energy when suits are self-supporting.

  13. Evaluation of an ompA-based phage-mediated DNA vaccine against Chlamydia abortus in piglets.

    Science.gov (United States)

    Ou, Changbo; Tian, Deyu; Ling, Yong; Pan, Qing; He, Qing; Eko, Francis O; He, Cheng

    2013-08-01

    Chlamydia abortus (C. abortus) is an obligate intracellular pathogen that causes abortion in pigs and poses a zoonotic risk in pregnant women. Although attenuated and inactivated vaccines are available, they do not provide complete protection in animals underlining the need to develop new vaccines. In this study, we tested the hypothesis that intramuscular immunization with an ompA-based phage-mediated DNA chlamydial vaccine candidate will induce significant antigen-specific cellular and humoral immune responses. Thus, groups of piglets (five per group) were immunized intramuscularly with the phage-MOMP vaccine (λ-MOMP) or a commercial live-attenuated vaccine (1B vaccine) or a GFP-expressing phage (λ-GFP) or phosphate buffered saline (PBS) (control) and antigen-specific cell-mediated and humoral immune responses were evaluated. By day 63 post-immunization, the λ-MOMP vaccine elicited significantly higher (Pabortus. Copyright © 2013 Elsevier B.V. All rights reserved.

  14. Evolutionary history of the OmpR/IIIA family of signal transduction two component systems in Lactobacillaceae and Leuconostocaceae

    Directory of Open Access Journals (Sweden)

    González-Candelas Fernando

    2011-02-01

    Full Text Available Abstract Background Two component systems (TCS are signal transduction pathways which typically consist of a sensor histidine kinase (HK and a response regulator (RR. In this study, we have analyzed the evolution of TCS of the OmpR/IIIA family in Lactobacillaceae and Leuconostocaceae, two families belonging to the group of lactic acid bacteria (LAB. LAB colonize nutrient-rich environments such as foodstuffs, plant materials and the gastrointestinal tract of animals thus driving the study of this group of both basic and applied interest. Results The genomes of 19 strains belonging to 16 different species have been analyzed. The number of TCS encoded by the strains considered in this study varied between 4 in Lactobacillus helveticus and 17 in Lactobacillus casei. The OmpR/IIIA family was the most prevalent in Lactobacillaceae accounting for 71% of the TCS present in this group. The phylogenetic analysis shows that no new TCS of this family has recently evolved in these Lactobacillaceae by either lineage-specific gene expansion or domain shuffling. Furthermore, no clear evidence of non-orthologous replacements of either RR or HK partners has been obtained, thus indicating that coevolution of cognate RR and HKs has been prevalent in Lactobacillaceae. Conclusions The results obtained suggest that vertical inheritance of TCS present in the last common ancestor and lineage-specific gene losses appear as the main evolutionary forces involved in their evolution in Lactobacillaceae, although some HGT events cannot be ruled out. This would agree with the genomic analyses of Lactobacillales which show that gene losses have been a major trend in the evolution of this group.

  15. Insights into PG-binding, conformational change, and dimerization of the OmpA C-terminal domains from Salmonella enterica serovar Typhimurium and Borrelia burgdorferi: Characterization of OmpA C-Terminal Domain

    Energy Technology Data Exchange (ETDEWEB)

    Tan, Kemin [Center for Structural Genomics of Infectious Diseases, University of Chicago, 5735 South Ellis Avenue Chicago Illinois 60637; Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Structural Biology Center, Biosciences, Argonne National Laboratory, Argonne Illinois 60439; Deatherage Kaiser, Brooke L. [National Security Directorate, Pacific Northwest National Laboratory, Richland Washington 99352; Wu, Ruiying [Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Cuff, Marianne [Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Fan, Yao [Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Bigelow, Lance [Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Jedrzejczak, Robert P. [Center for Structural Genomics of Infectious Diseases, University of Chicago, 5735 South Ellis Avenue Chicago Illinois 60637; Adkins, Joshua N. [Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland Washington 99352; Cort, John R. [Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland Washington 99352; Babnigg, Gyorgy [Center for Structural Genomics of Infectious Diseases, University of Chicago, 5735 South Ellis Avenue Chicago Illinois 60637; Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Joachimiak, Andrzej [Center for Structural Genomics of Infectious Diseases, University of Chicago, 5735 South Ellis Avenue Chicago Illinois 60637; Midwest Center for Structural Genomics, Argonne National Laboratory, Argonne Illinois 60439; Structural Biology Center, Biosciences, Argonne National Laboratory, Argonne Illinois 60439

    2017-06-19

    S. Typhimurium can induce both humoral and cell-mediated responses when establishing itself in the host. These responses are primarily stimulated against the lipopolysaccharide and major outer membrane (OM) proteins. OmpA is one of these major OM proteins. It comprises a N-terminal eight-stranded b-barrel trans membrane domain and a C-terminal domain (OmpACTD). The OmpACTD and its homologs are believed to bind to peptidoglycan (PG) within the periplasm, maintaining bacterial osmotic homeostasis and modulating the permeability and integrity of the OM. Here we present the first crystal structures of the OmpACTD from two pathogens: S. Typhimurium (STOmpACTD) in open and closed forms and causative agent of Lyme Disease Borrelia burgdorferi (BbOmpACTD), in closed form. In the open form of STOmpACTD, an aspartic acid residue from a long b2-a3 loop points into the binding pocket, suggesting that an anion group such as a carboxylate group from PG is favored at the binding site. In the closed form of STOmpACTD and in the structure of BbOmpACTD, a sulfate group from the crystallization buffer is tightly bound at the binding site. The differences between the closed and open forms of STOmpACTD, suggest a large conformational change that includes an extension of a3 helix by ordering a part of b2-a3 loop. We propose that the sulfate anion observed in these structures mimics the carboxylate group of PG when bound to STOmpACTD suggesting PG-anchoring mechanism. In addition, the binding of PG or a ligand mimic may enhance dimerization of STOmpACTD, or possibly that of full length STOmpA.

  16. NIH bows to part of Rifkin suit.

    Science.gov (United States)

    Sun, M

    1984-11-30

    Having lost a round in its legal battle with Jeremy Rifkin over field tests of genetically engineered bacteria, the National Institutes of Health will conduct the simpler of two ecological analyses required by the National Environmental Policy Act on three proposed experiments. In May 1984 a federal district court ruling halted a University of California field test pending a decision on Rifkin's 1983 suit, which alleged that NIH had violated the Act by approving experiments without studying the ecological consequences. Still to be decided by the U.S. Court of Appeals is whether NIH must also issue full-scale environmental impact statements.

  17. Geophysical characterization from Itu intrusive suite

    International Nuclear Information System (INIS)

    Pascholati, M.E.

    1989-01-01

    The integrated use of geophysical, geological, geochemical, petrographical and remote sensing data resulted in a substantial increase in the knowledge of the Itu Intrusive Suite. The main geophysical method was gamma-ray spectrometry together with fluorimetry and autoradiography. Three methods were used for calculation of laboratory gamma-ray spectrometry data. For U, the regression method was the best one. For K and Th, equations system and absolute calibration presented the best results. Surface gamma-ray spectrometry allowed comparison with laboratory data and permitted important contribution to the study of environmental radiation. (author)

  18. Implementing Sentinels in the TARGIT BI Suite

    DEFF Research Database (Denmark)

    Middelfart, Morten; Pedersen, Torben Bach

    2011-01-01

    This paper describes the implementation of socalled sentinels in the TARGIT BI Suite. Sentinels are a novel type of rules that can warn a user if one or more measure changes in a multi-dimensional data cube are expected to cause a change to another measure critical to the user. Sentinels notify u...... pattern mining or correlation techniques. We demonstrate, through extensive experiments, that mining and usage of sentinels is feasible with good performance for the typical users on a real, operational data warehouse....

  19. ANALYSIS OF DESIGN ELEMENTS IN SKI SUITS

    OpenAIRE

    Çileroğlu, Birsen; Kelleci Özeren, Figen; Kıvılcımlar, İnci Seda

    2015-01-01

    Popularity of Ski Sport in 19th century necessitated a new perspective on protective skiing clothing against the mountain climates and excessive cold. Winter clothing were the basis of ski attire during this period.  By the beginning of 20th century lining cloth were used to minimize the wind effect. The difference between the men and women’s ski attire of the time consisted of a knee-length skirts worn over the golf trousers.  Subsequent to the First World War, skiing suit models were influe...

  20. Durable Suit Bladder with Improved Water Permeability for Pressure and Environment Suits

    Science.gov (United States)

    Bue, Grant C.; Kuznetz, Larry; Orndoff, Evelyne; Tang, Henry; Aitchison, Lindsay; Ross, Amy

    2009-01-01

    Water vapor permeability is shown to be useful in rejecting heat and managing moisture accumulation in launch-and-entry pressure suits. Currently this is accomplished through a porous Gortex layer in the Advanced Crew and Escape Suit (ACES) and in the baseline design of the Constellation Suit System Element (CSSE) Suit 1. Non-porous dense monolithic membranes (DMM) that are available offer potential improvements for water vapor permeability with reduced gas leak. Accordingly, three different pressure bladder materials were investigated for water vapor permeability and oxygen leak: ElasthaneTM 80A (thermoplastic polyether urethane) provided from stock polymer material and two custom thermoplastic polyether urethanes. Water vapor, carbon dioxide and oxygen permeability of the DMM's was measured in a 0.13 mm thick stand-alone layer, a 0.08 mm and 0.05 mm thick layer each bonded to two different nylon and polyester woven reinforcing materials. Additional water vapor permeability and mechanical compression measurements were made with the reinforced 0.05 mm thick layers, further bonded with a polyester wicking and overlaid with moistened polyester fleece thermal underwear .This simulated the pressure from a supine crew person. The 0.05 mm thick nylon reinforced sample with polyester wicking layer was further mechanically tested for wear and abrasion. Concepts for incorporating these materials in launch/entry and Extravehicular Activity pressure suits are presented.

  1. Integrated Instrument Simulator Suites for Earth Science

    Science.gov (United States)

    Tanelli, Simone; Tao, Wei-Kuo; Matsui, Toshihisa; Hostetler, Chris; Hair, John; Butler, Carolyn; Kuo, Kwo-Sen; Niamsuwan, Noppasin; Johnson, Michael P.; Jacob, Joseph C.; hide

    2012-01-01

    The NASA Earth Observing System Simulators Suite (NEOS3) is a modular framework of forward simulations tools for remote sensing of Earth's Atmosphere from space. It was initiated as the Instrument Simulator Suite for Atmospheric Remote Sensing (ISSARS) under the NASA Advanced Information Systems Technology (AIST) program of the Earth Science Technology Office (ESTO) to enable science users to perform simulations based on advanced atmospheric and simple land surface models, and to rapidly integrate in a broad framework any experimental or innovative tools that they may have developed in this context. The name was changed to NEOS3 when the project was expanded to include more advanced modeling tools for the surface contributions, accounting for scattering and emission properties of layered surface (e.g., soil moisture, vegetation, snow and ice, subsurface layers). NEOS3 relies on a web-based graphic user interface, and a three-stage processing strategy to generate simulated measurements. The user has full control over a wide range of customizations both in terms of a priori assumptions and in terms of specific solvers or models used to calculate the measured signals.This presentation will demonstrate the general architecture, the configuration procedures and illustrate some sample products and the fundamental interface requirements for modules candidate for integration.

  2. In-silico design, expression, and purification of novel chimeric Escherichia coli O157:H7 OmpA fused to LTB protein in Escherichia coli.

    Directory of Open Access Journals (Sweden)

    Aytak Novinrooz

    Full Text Available E. coli O157:H7, one of the major EHEC serotypes, is capable of developing bloody diarrhea, hemorrhagic colitis (HC, and fatal hemolytic uremic syndrome (HUS and is accompanied by high annual economic loss worldwide. Due to the increased risk of HC and HUS development following antibiotic therapy, the prevention of infections caused by this pathogen is considered to be one of the most effective ways of avoiding the consequences of this infection. The main aim of the present study was to design, express, and purify a novel chimeric protein to develope human vaccine candidate against E. coli O157:H7 containing loop 2-4 of E. coli O157:H7, outer membrane protein A (OmpA, and B subunit of E. coli heat labile enterotoxin (LTB which are connected by a flexible peptide linker. Several online databases and bioinformatics software were utilized to choose the peptide linker among 537 analyzed linkers, design the chimeric protein, and optimize the codon of the relative gene encoding this protein. Subsequently, the recombinant gene encoding OmpA-LTB was synthesized and cloned into pET-24a (+ expression vector and transferred to E. coli BL21(DE3 cells. The expression of OmpA-LTB chimeric protein was then carried out by induction of cultured E. coli Bl21 (DE3 cells with 1mM isopropyl-β-D-thiogalactopyranoside (IPTG. The purification of OmpA-LTB was then performed by nickel affinity chromatography. Expression and purification were analyzed by sodium dodecyl sulphate poly acrylamide gel electrophoresis. Moreover, the identity of the expressed protein was analyzed by western blotting. SDS-PAGE and western immunoblotting confirmed the successful expression of a 27 KDa recombinant protein after 24 hours at 37°C post-IPTG induction. OmpA-LTB was then successfully purified, using nickel affinity chromatography under denaturing conditions. The yield of purification was 12 mg per liter of culture media. Ultimately, we constructed the successful design and efficient

  3. Soil Aquifer Treatment (SAT) and Constructed Wetlands (CW) Applications for Nutrients and Organic Micropollutants (OMPs) Attenuation Using Primary and Secondary Wastewater Effluents

    KAUST Repository

    Hamadeh, Ahmed F.

    2014-06-01

    Constructed wetlands (CW) and soil aquifer treatment (SAT) represent natural wastewater treatment systems (NWTSs). The high costs of conventional wastewater treatment techniques encourage more studies to investigate lower cost treatment methods which make these appropriate for developing and also in developed countries. The main objective of this research was to investigate the removals of nutrients and organic micropollutants (OMPs) through SAT, CW and the CW-SAT hybrid system. CWs are an efficient technology to purify and remove different nutrients as well as OMPs from wastewater. They removed most of the dissolved organic matter (DOC), total nitrogen (TN), ammonium and phosphate. Furthermore, CWs aeration could be used as one of the alternatives to reduce CWs footprint by around 10%. The vegetation in CWs plays an essential role in the treatment especially for nitrogen and phosphate removals, it is responsible for the removal of 15%, 55%, 38%, and 22% for TN, dissolved organic nitrogen (DON), nitrate and phosphate, respectively. CWs achieved a very high removal for some OMPs; they attenuated acetaminophen, caffeine, fluoxetine and trimethoprim (>90%) under different redox conditions. Moreover, it was found that increasing temperature (up to 36 C) could enhance the removals of atenolol, caffeine, DEET and trimethoprim by 17%, 14%, 28% and 45%, respectively. On the other hand, some OMPs, were found to be removed by vegetation such as: acetaminophen, caffeine, fluoxetine, sulfamethoxazole, and trimethoprim. Moreover, atenolol, caffeine, fluoxetine and trimethoprim, showed high removal (>80%) through SAT system. It was also found that, temperature increasing and using primary instead of secondary effluent could enhance the removal of some OMPs. The CWs performance study showed that these systems are adapted to the prevailing extreme arid conditions and the average percent removals are about, 88%, 96%, 98%, 98% and 92%, for COD, BOD and TSS, ammonium and phosphate

  4. MODEL: A software suite for data acquisition

    Energy Technology Data Exchange (ETDEWEB)

    Sendall, D M; Boissat, C; Bozzoli, W; Burkimsher, P; Jones, R; Matheys, J P; Mornacchi, G; Nguyen, T; Vyvre, P vande; Vascotto, A; Weaver, D [European Organization for Nuclear Research, Geneva (Switzerland). DD Div.

    1989-12-01

    MODEL is a new suite of modular data-acquisition software. It is aimed at the needs of LEP experiments, and is also general enough to be more widely used. It can accomodate a variety of users styles. It runs on a set of loosely coupled processors, and makes use of the remote procedure call technique. Implemented originally for the VAX family, some of its services have already been extended to other systems, including embedded microprocessors. The software modules available include facilities for data-flow management, a framework for monitoring programs, a window-oriented human interface, an error message utility, a process control utility and a run control scheme. It is already in use in a variety of experiments, and is still under development in the light of user experience. (orig.).

  5. UniPOPS: Unified data reduction suite

    Science.gov (United States)

    Maddalena, Ronald J.; Garwood, Robert W.; Salter, Christopher J.; Stobie, Elizabeth B.; Cram, Thomas R.; Morgan, Lorrie; Vance, Bob; Hudson, Jerome

    2015-03-01

    UniPOPS, a suite of programs and utilities developed at the National Radio Astronomy Observatory (NRAO), reduced data from the observatory's single-dish telescopes: the Tucson 12-m, the Green Bank 140-ft, and archived data from the Green Bank 300-ft. The primary reduction programs, 'line' (for spectral-line reduction) and 'condar' (for continuum reduction), used the People-Oriented Parsing Service (POPS) as the command line interpreter. UniPOPS unified previous analysis packages and provided new capabilities; development of UniPOPS continued within the NRAO until 2004 when the 12-m was turned over to the Arizona Radio Observatory (ARO). The submitted code is version 3.5 from 2004, the last supported by the NRAO.

  6. Vadose zone flow convergence test suite

    Energy Technology Data Exchange (ETDEWEB)

    Butcher, B. T. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2017-06-05

    Performance Assessment (PA) simulations for engineered disposal systems at the Savannah River Site involve highly contrasting materials and moisture conditions at and near saturation. These conditions cause severe convergence difficulties that typically result in unacceptable convergence or long simulation times or excessive analyst effort. Adequate convergence is usually achieved in a trial-anderror manner by applying under-relaxation to the Saturation or Pressure variable, in a series of everdecreasing RELAxation values. SRNL would like a more efficient scheme implemented inside PORFLOW to achieve flow convergence in a more reliable and efficient manner. To this end, a suite of test problems that illustrate these convergence problems is provided to facilitate diagnosis and development of an improved convergence strategy. The attached files are being transmitted to you describing the test problem and proposed resolution.

  7. Metallogenic aspects of Itu intrusive suite

    International Nuclear Information System (INIS)

    Amaral, G.; Pascholati, E.M.

    1990-01-01

    The integrated use of geological, geochemical, geophysical and remote sensing data is providing interesting new information on the metallogenic characteristics of the Itu Intrusive Suite. During World War II, up to 1959, a wolframite deposit was mined near the border of the northernmost body (Itupeva Granite). This deposit is formed by greisen veins associated with cassiterite and topaz, clearly linked with later phases of magmatic differentiation. Generally those veins are related to hydrothermal alteration of the granites and the above mentioned shear zone. U, Th and K determinations by field and laboratory gammaspectrometry were used for regional distribution analysis of those elements and its ratios and calculation of radioactivity heat production. In this aspects, the Itupeva Granite is the hottest and presents several anomalies in the Th/U ratio, indicative of late or post magmatic oxidation processes. (author)

  8. Specification for the VERA Depletion Benchmark Suite

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Kang Seog [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2015-12-17

    CASL-X-2015-1014-000 iii Consortium for Advanced Simulation of LWRs EXECUTIVE SUMMARY The CASL neutronics simulator MPACT is under development for the neutronics and T-H coupled simulation for the pressurized water reactor. MPACT includes the ORIGEN-API and internal depletion module to perform depletion calculations based upon neutron-material reaction and radioactive decay. It is a challenge to validate the depletion capability because of the insufficient measured data. One of the detoured methods to validate it is to perform a code-to-code comparison for benchmark problems. In this study a depletion benchmark suite has been developed and a detailed guideline has been provided to obtain meaningful computational outcomes which can be used in the validation of the MPACT depletion capability.

  9. Outer membrane protein A (OmpA of Shigella flexneri 2a induces TLR2-mediated activation of B cells: involvement of protein tyrosine kinase, ERK and NF-κB.

    Directory of Open Access Journals (Sweden)

    Rajsekhar Bhowmick

    Full Text Available B cells are critically important in combating bacterial infections and their differentiation into plasma cells and memory cells aids bacterial clearance and long-lasting immunity conferred by essentially all vaccines. Outer membrane protein A (OmpA of Shigella flexneri 2a has been demonstrated to induce the production of IgG and IgA in vivo following immunization of mice through intranasal route, but the direct involvement of B cells in OmpA-mediated immune regulation was not determined. Consequently, we investigated whether OmpA can modulate B cell functions and identified the molecular events involved in OmpA-induced B cell immune response in vitro. We show that OmpA of S. flexneri 2a activates B cells to produce protective cytokines, IL-6 and IL-10 as well as facilitates their differentiation into antibody secreting cells (ASCs. The immunostimulatory properties of OmpA are attributed to the increased surface expression of MHCII and CD86 on B cells. We also report here that B cell activation by OmpA is mediated strictly through recognition by TLR2, resulting in initiation of cascades of signal transduction events, involving increased phosphorylation of protein tyrosine kinases (PTKs, ERK and IκBα, leading to nuclear translocation of NF-κB. Importantly, a TLR2 antibody diminishes OmpA-induced upregulation of MHCII and CD86 on B cell surface as well as significantly inhibits B cell differentiation and cytokine secretion. Furthermore, we illustrate that B cell differentiation into ASCs and induction of cytokine secretion by OmpA are dependent on PTKs activity. Moreover, we identify that OmpA-induced B cell differentiation is entirely dependent on ERK pathway, whereas both NF-κB and ERK are essential for cytokine secretion by B cells. Overall, our data demonstrate that OmpA of S. flexneri 2a amplifies TLR signaling in B cells and triggers B cell immune response, which is critical for the development of an effective adaptive immunity to an

  10. Instrumented Suit Hard Upper Torso (HUT) for Ergonomic Assessment

    Data.gov (United States)

    National Aeronautics and Space Administration — It is well known that the EVA suit (EMU) has the potential to cause crew injury and decreased performance. Engineering data on the suit interaction of the human...

  11. Variable Vector Countermeasure Suit for Space Habitation and Exploration

    Data.gov (United States)

    National Aeronautics and Space Administration — The "Variable Vector Countermeasure Suit (V2Suit) for Space Habitation and Exploration" is a visionary system concept that will revolutionize space missions by...

  12. Development of a Fan for Future Space Suit Applications

    Science.gov (United States)

    Paul. Heather L.; Converse, David; Dionne, Steven; Moser, Jeff

    2010-01-01

    NASA's next generation space suit system will place new demands on the fan used to circulate breathing gas through the ventilation loop of the portable life support system. Long duration missions with frequent extravehicular activities (EVAs), the requirement for significant increases in reliability and durability, and a mission profile that imposes strict limits on weight, volume and power create the basis for a set of requirements that demand more performance than is available from existing fan designs. This paper describes the development of a new fan to meet these needs. A centrifugal fan was designed with a normal operating speed of approximately 39,400 rpm to meet the ventilation flow requirements while also meeting the aggressive minimal packaging, weight and power requirements. The prototype fan also operates at 56,000 rpm to satisfy a second operating condition associated with a single fan providing ventilation flow to two spacesuits connected in series. This fan incorporates a novel nonmetallic "can" to keep the oxygen flow separate from the motor electronics, thus eliminating ignition potential. The nonmetallic can enables a small package size and low power consumption. To keep cost and schedule within project bounds a commercial motor controller was used. The fan design has been detailed and implemented using materials and approaches selected to address anticipated mission needs. Test data is presented to show how this fan performs relative to anticipated ventilation requirements for the EVA portable life support system. Additionally, data is presented to show tolerance to anticipated environmental factors such as acoustics, shock, and vibration. Recommendations for forward work to progress the technology readiness level and prepare the fan for the next EVA space suit system are also discussed.

  13. 鸭多杀性巴氏杆菌外膜蛋白A基因表达及抗原性鉴定%Cloning and Expression of the omp A Gene of pasteurella multocida

    Institute of Scientific and Technical Information of China (English)

    孙龚; 郭东春; 刘家森; 姜骞; 司昌德; 林欢; 韩凌霞; 崔玉东; 曲连东

    2010-01-01

    根据已发表的Pm 70株的序列(GenBank登录号:AE004439)设计了两对引物,用PCR方法扩增了鸭多杀性巴氏杆菌标准株C48-102的外膜蛋白基因A(omp A),扩增的片段为1 062 bp.将测序结果与GenBank中多杀性巴氏杆菌P52、PM 70、T931317、194289的omp A序列比对结果表明,核苷酸水平上同源性为89.0%~98.9%;在氨基酸水平上同源性为90.7%~99.2%.全omp A序列在大肠杆菌中干扰蛋白的正常表达,因此截断omp A蛋白的信号肽序列,将去信号肽的omp A基因插入到pPRO-EX-Htb载体上,构建了原核表达载体Htb-omp A,转化BL21,并诱导表达,SDS-PAGE结果表明,表达蛋白约为35 kDa,与预期的分子量大小相符.Western-blot结果表明,表达的蛋白具有良好的抗原活性.本研究重组蛋白omp A的获得,为敲除omp A基因多杀性巴氏杆菌突变株的血清学检测奠定基础.

  14. Characterization of novel OmpA-like protein of Leptospira interrogans that binds extracellular matrix molecules and plasminogen.

    Science.gov (United States)

    Oliveira, Rosane; de Morais, Zenaide Maria; Gonçales, Amane Paldes; Romero, Eliete Caló; Vasconcellos, Silvio Arruda; Nascimento, Ana L T O

    2011-01-01

    Leptospira interrogans is the etiological agent of leptospirosis, a zoonotic disease of human and veterinary concern. The identification of novel proteins that mediate host-pathogen interactions is important for understanding the bacterial pathogenesis as well as to identify protective antigens that would help fight the disease. We describe in this work the cloning, expression, purification and characterization of three predicted leptospiral membrane proteins, LIC10258, LIC12880 (Lp30) and LIC12238. We have employed Escherichia coli BL21 (SI) strain as a host expression system. Recently, we have identified LIC12238 as a plasminogen (PLG)-binding receptor. We show now that Lp30 and rLIC10258 are also PLG-receptors of Leptospira, both exhibiting dose-dependent and saturating binding (K(D), 68.8±25.2 nM and 167.39±60.1 nM, for rLIC10258 and rLIC12880, respectively). In addition, LIC10258, which is a novel OmpA-like protein, binds laminin and plasma fibronectin ECM molecules and hence, it was named Lsa66 (Leptospiral surface adhesin of 66 kDa). Binding of Lsa66 to ECM components was determined to be specific, dose-dependent and saturable, with a K(D) of 55.4±15.9 nM to laminin and of 290.8±11.8 nM to plasma fibronectin. Binding of the recombinant proteins to PLG or ECM components was assessed by using antibodies against each of the recombinant proteins obtained in mice and confirmed by monoclonal anti-polyhistidine antibodies. Lsa66 caused partial inhibition on leptospiral adherence to immobilized ECM and PLG. Moreover, this adhesin and rLIC12238 are recognized by antibodies in serum samples of confirmed leptospirosis cases. Thus, Lsa66 is a novel OmpA-like protein with dual activity that may promote the attachment of Leptospira to host tissues and may contribute to the leptospiral invasion. To our knowledge, this is the first leptospiral protein with ECM and PLG binding properties reported to date.

  15. Characterization of novel OmpA-like protein of Leptospira interrogans that binds extracellular matrix molecules and plasminogen.

    Directory of Open Access Journals (Sweden)

    Rosane Oliveira

    Full Text Available Leptospira interrogans is the etiological agent of leptospirosis, a zoonotic disease of human and veterinary concern. The identification of novel proteins that mediate host-pathogen interactions is important for understanding the bacterial pathogenesis as well as to identify protective antigens that would help fight the disease. We describe in this work the cloning, expression, purification and characterization of three predicted leptospiral membrane proteins, LIC10258, LIC12880 (Lp30 and LIC12238. We have employed Escherichia coli BL21 (SI strain as a host expression system. Recently, we have identified LIC12238 as a plasminogen (PLG-binding receptor. We show now that Lp30 and rLIC10258 are also PLG-receptors of Leptospira, both exhibiting dose-dependent and saturating binding (K(D, 68.8±25.2 nM and 167.39±60.1 nM, for rLIC10258 and rLIC12880, respectively. In addition, LIC10258, which is a novel OmpA-like protein, binds laminin and plasma fibronectin ECM molecules and hence, it was named Lsa66 (Leptospiral surface adhesin of 66 kDa. Binding of Lsa66 to ECM components was determined to be specific, dose-dependent and saturable, with a K(D of 55.4±15.9 nM to laminin and of 290.8±11.8 nM to plasma fibronectin. Binding of the recombinant proteins to PLG or ECM components was assessed by using antibodies against each of the recombinant proteins obtained in mice and confirmed by monoclonal anti-polyhistidine antibodies. Lsa66 caused partial inhibition on leptospiral adherence to immobilized ECM and PLG. Moreover, this adhesin and rLIC12238 are recognized by antibodies in serum samples of confirmed leptospirosis cases. Thus, Lsa66 is a novel OmpA-like protein with dual activity that may promote the attachment of Leptospira to host tissues and may contribute to the leptospiral invasion. To our knowledge, this is the first leptospiral protein with ECM and PLG binding properties reported to date.

  16. Carbapenem and cefoxitin resistance of Klebsiella pneumoniae strains associated with porin OmpK36 loss and DHA-1 β-lactamase production

    Directory of Open Access Journals (Sweden)

    Weifeng Shi

    2013-01-01

    Full Text Available Clinical isolates of carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae strains are being increased worldwide. Five pan-resistant K. pneumoniae strains have been isolated from respiratory and ICU wards in a Chinese hospital, and reveal strong resistance to all β-lactams, fluoroquinolones and aminoglycosides. Totally 27 β-lactamase genes and 2 membrane pore protein (porin genes in 5 K. pneumoniae strains were screened by polymerase chain reaction (PCR. The results indicated that all of 5 K. pneumoniae strains carried blaTEM-1 and blaDHA-1 genes, as well as base deletion and mutation of OmpK35 or OmpK36 genes. Compared with carbapenem-sensitive isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, the resistant isolates markedly lacked the protein band of 34-40 kDa, which might be the outer membrane proteins of OmpK36 according to the electrophoresis mobility. In addition, the conjugation test was confirmed that blaDHA-1 mediated by plasmids could be transferred between resistant and sensitive strains. When reserpine (30 µg/mL and carbonyl cyanide m-chlorophenylhydrazone (CCCP (50 µg/mL were added in imipenem and meropenem, the MICs had no change against K. pneumoniae strains. These results suggest that both DHA-1 β-lactamase and loss or deficiency of porin OmpK36 may be the main reason for the cefoxitin and carbapenem resistance in K. pneumoniae strains in our hospital.

  17. Improving Fab' fragment retention in an autonucleolytic Escherichia coli strain by swapping periplasmic nuclease translocation signal from OmpA to DsbA.

    Science.gov (United States)

    Schofield, Desmond M; Sirka, Ernestas; Keshavarz-Moore, Eli; Ward, John M; Nesbeth, Darren N

    2017-12-01

    To reduce unwanted Fab' leakage from an autonucleolytic Escherichia coli strain, which co-expresses OmpA-signalled Staphylococcal nuclease and Fab' fragment in the periplasm, by substituting in Serratial nuclease and the DsbA periplasm translocation signal as alternatives. We attempted to genetically fuse a nuclease from Serratia marcescens to the OmpA signal peptide but plasmid construction failed, possibly due to toxicity of the resultant nuclease. Combining Serratial nuclease to the DsbA signal peptide was successful. The strain co-expressing this nuclease and periplasmic Fab' grew in complex media and exhibited nuclease activity detectable by DNAse agar plate but its growth in defined medium was retarded. Fab' coexpression with Staphylococcal nuclease fused to the DsbA signal peptide resulted in cells exhibiting nuclease activity and growth in defined medium. In cultivation to high cell density in a 5 l bioreactor, DsbA-fused Staphylococcal nuclease co-expression coincided with reduced Fab' leakage relative to the original autonucleolytic Fab' strain with OmpA-fused staphylococcal nuclease. We successfully rescued Fab' leakage back to acceptable levels and established a basis for future investigation of the linkage between periplasmic nuclease expression and leakage of co-expressed periplasmic Fab' fragment to the surrounding growth media.

  18. High-resolution diffraction from crystals of a membrane-protein complex: bacterial outer membrane protein OmpC complexed with the antibacterial eukaryotic protein lactoferrin

    International Nuclear Information System (INIS)

    Sundara Baalaji, N.; Acharya, K. Ravi; Singh, T. P.; Krishnaswamy, S.

    2005-01-01

    Crystals of the complex formed between the bacterial membrane protein OmpC and the antibacterial protein lactoferrin suitable for high-resolution structure determination have been obtained. The crystals belong to the hexagonal space group P6, with unit-cell parameters a = b = 116.3, c = 152.4 Å. Crystals of the complex formed between the outer membrane protein OmpC from Escherichia coli and the eukaryotic antibacterial protein lactoferrin from Camelus dromedarius (camel) have been obtained using a detergent environment. Initial data processing suggests that the crystals belong to the hexagonal space group P6, with unit-cell parameters a = b = 116.3, c = 152.4 Å, α = β = 90, γ = 120°. This indicated a Matthews coefficient (V M ) of 3.3 Å 3 Da −1 , corresponding to a possible molecular complex involving four molecules of lactoferrin and two porin trimers in the unit cell (4832 amino acids; 533.8 kDa) with 63% solvent content. A complete set of diffraction data was collected to 3 Å resolution at 100 K. Structure determination by molecular replacement is in progress. Structural study of this first surface-exposed membrane-protein complex with an antibacterial protein will provide insights into the mechanism of action of OmpC as well as lactoferrin

  19. The fitness costs and trade-off shapes associated with the exclusion of nine antibiotics by OmpF porin channels.

    Science.gov (United States)

    Phan, Katherine; Ferenci, Thomas

    2017-06-01

    The trade-off relationship between antibiotic exclusion and nutrient access across the Gram-negative outer membrane is determined by structural constraints in porin channels. The precise nutritional cost of exclusion is unknown for different antibiotics, as are the shapes of the nutrition-susceptibility trade-off. Using a library of 10 engineered isogenic Escherichia coli strains with structural modifications of OmpF porin expressed at a constant level, susceptibilities were measured for nine antibiotics and the nutritional fitness costs estimated by competitions in chemostats. Different antibiotics exhibited a remarkably varied range of geometries in the nutrition-susceptibility trade-off, including convex, concave and sigmoidal trade-off shapes. The trade-off patterns predict the possibility of adaptations in contributing to antibiotic resistance; exclusion of amoxicillin or trimethoprim in ompF mutants can occur with little loss of fitness whereas kanamycin and streptomycin exclusion has a high cost. Some individual OmpF changes even allow positive correlations (trade-ups), resulting in increased fitness and decreased susceptibility specifically to cephalexin or ciprofloxacin. The surprising plasticity of the nutrition-exclusion relationship means that there are no generalisable rules that apply to decreasing susceptibility for all antibiotics. The protein changes are exquisitely specific in determining nutritional fitness and adaptive outcomes in a structural constraint trade-off.

  20. Down-regulation of outer membrane proteins by noncoding RNAs: unraveling the cAMP-CRP- and sigmaE-dependent CyaR-ompX regulatory case

    DEFF Research Database (Denmark)

    Johansen, Jesper; Eriksen, Maiken; Kallipolitis, Birgitte

    2008-01-01

    is sufficient to trigger the envelope stress response. Recent work indicates that small Hfq-binding RNAs play a major role in maintaining envelope homeostasis and, so far, two sigma(E)-dependent small noncoding RNAs (sRNAs), MicA and RybB, have been shown to facilitate rapid removal of multiple omp transcripts......The sigma(E) (extracytoplasmic stress response sigma factor in Escherichia coli) signaling system of Gram-negative bacteria plays an essential role in the maintenance of the extracytoplasmic compartment. Upon induction of this system, approximately 100 genes are up-regulated. The majority...... is also up-regulated, directly or indirectly, by sigma(E). In addition, this work identified MicA as a factor that cooperates in the negative control of ompX expression. The conservation of CyaR, MicA, RybB, and their targets suggests that the omp mRNA-sRNA regulatory network is an integral part...

  1. Chlamydia trachomatis ompA genotypes in male patients with urethritis in Greece: conservation of the serovar distribution and evidence for mixed infections with Chlamydophila abortus.

    Science.gov (United States)

    Psarrakos, Panagiotis; Papadogeorgakis, Eleni; Sachse, Konrad; Vretou, Evangelia

    2011-08-01

    PCR amplification and nucleotide sequencing of the ompA gene of Chlamydia trachomatis were used to determine the prevalence and distribution of genotypes in 51 urine and urethral specimens from Greek male patients with urethritis, that were positive by the COBAS Amplicor test. A single C. trachomatis serovar was identified in 43 of the 51 amplified samples. Serovars F and E were the most prevalent (both 12, 28%), followed by D (9, 21%), G (4, 9%), B and K (both 2, 5%) and H and J (both 1, 2%). Over one third of the samples bared a variant ompA genotype that had been previously identified in other areas worldwide. Two results in this study, both observed for the first time, were of particular interest. First, the emergence of the unique variant genotype D/Ep6 (X77364.2) identified in 3 urethral samples. Second, the ompA genotype OCLH196 of the animal pathogen Chlamydophila abortus as well as a 23S rRNA gene fragment of this species detected by the assay ArrayTube™ was found in 7 urethral samples. The implications resulting from this observation for the health of the general population are discussed. Copyright © 2011 Elsevier Ltd. All rights reserved.

  2. Improved airline-type supplied-air plastic suit

    International Nuclear Information System (INIS)

    Jolley, L. Jr.; Zippler, D.B.; Cofer, C.H.; Harper, J.A.

    1978-06-01

    Two piece supplied-air plastic suits are used extensively at the Savannah River Plant for personnel protection against inhalation of airborne plutonium and tritium. Worker comfort and noise level problems gave impetus to development of an improved suit and aid distribution system. The resulting plastic suit and development work are discussed. The plastic suit unit cost is less than $20, the hearing zone noise level is less than 75 dBA, protection factors exceed 10,000, and user comfort is approved. This suit is expected to meet performance requirements for unrestricted use

  3. [Antigravity suit used for neurosurgical operations in sitting position].

    Science.gov (United States)

    Szpiro-Zurkowska, A; Milczarek, Z; Marchel, A; Jagielski, J

    1996-01-01

    The aviator's antigravity suit (G-suit) was used for 40 operations on neurosurgical patients operated on in sitting position. The G-suit was filled with air to 0.2 atmosphere (20 kPa) pressure in 26 cases, and 0.3 atm. (30 kPa) in 14 cases. In all cases G-suit filling was followed by central venous pressure rise and mean arterial pressure rise. Venous air embolism was found in 5 (12.5%) patients. No other complications connected with the use of G-suit were observed.

  4. STELLTRANS: A Transport Analysis Suite for Stellarators

    Science.gov (United States)

    Mittelstaedt, Joseph; Lazerson, Samuel; Pablant, Novimir; Weir, Gavin; W7-X Team

    2016-10-01

    The stellarator transport code STELLTRANS allows us to better analyze the power balance in W7-X. Although profiles of temperature and density are measured experimentally, geometrical factors are needed in conjunction with these measurements to properly analyze heat flux densities in stellarators. The STELLTRANS code interfaces with VMEC to find an equilibrium flux surface configuration and with TRAVIS to determine the RF heating and current drive in the plasma. Stationary transport equations are then considered which are solved using a boundary value differential equation solver. The equations and quantities considered are averaged over flux surfaces to reduce the system to an essentially one dimensional problem. We have applied this code to data from W-7X and were able to calculate the heat flux coefficients. We will also present extensions of the code to a predictive capacity which would utilize DKES to find neoclassical transport coefficients to update the temperature and density profiles.

  5. Features of Two New Proteins with OmpA-Like Domains Identified in the Genome Sequences of Leptospira interrogans

    Science.gov (United States)

    Teixeira, Aline F.; de Morais, Zenaide M.; Kirchgatter, Karin; Romero, Eliete C.; Vasconcellos, Silvio A.; Nascimento, Ana Lucia T. O.

    2015-01-01

    Leptospirosis is an acute febrile disease caused by pathogenic spirochetes of the genus Leptospira. It is considered an important re-emerging infectious disease that affects humans worldwide. The knowledge about the mechanisms by which pathogenic leptospires invade and colonize the host remains limited since very few virulence factors contributing to the pathogenesis of the disease have been identified. Here, we report the identification and characterization of two new leptospiral proteins with OmpA-like domains. The recombinant proteins, which exhibit extracellular matrix-binding properties, are called Lsa46 - LIC13479 and Lsa77 - LIC10050 (Leptospiral surface adhesins of 46 and 77 kDa, respectively). Attachment of Lsa46 and Lsa77 to laminin was specific, dose dependent and saturable, with KD values of 24.3 ± 17.0 and 53.0 ± 17.5 nM, respectively. Lsa46 and Lsa77 also bind plasma fibronectin, and both adhesins are plasminogen (PLG)-interacting proteins, capable of generating plasmin (PLA) and as such, increase the proteolytic ability of leptospires. The proteins corresponding to Lsa46 and Lsa77 are present in virulent L. interrogans L1-130 and in saprophyte L. biflexa Patoc 1 strains, as detected by immunofluorescence. The adhesins are recognized by human leptospirosis serum samples at the onset and convalescent phases of the disease, suggesting that they are expressed during infection. Taken together, our data could offer valuable information to the understanding of leptospiral pathogenesis. PMID:25849456

  6. Features of two new proteins with OmpA-like domains identified in the genome sequences of Leptospira interrogans.

    Directory of Open Access Journals (Sweden)

    Aline F Teixeira

    Full Text Available Leptospirosis is an acute febrile disease caused by pathogenic spirochetes of the genus Leptospira. It is considered an important re-emerging infectious disease that affects humans worldwide. The knowledge about the mechanisms by which pathogenic leptospires invade and colonize the host remains limited since very few virulence factors contributing to the pathogenesis of the disease have been identified. Here, we report the identification and characterization of two new leptospiral proteins with OmpA-like domains. The recombinant proteins, which exhibit extracellular matrix-binding properties, are called Lsa46 - LIC13479 and Lsa77 - LIC10050 (Leptospiral surface adhesins of 46 and 77 kDa, respectively. Attachment of Lsa46 and Lsa77 to laminin was specific, dose dependent and saturable, with KD values of 24.3 ± 17.0 and 53.0 ± 17.5 nM, respectively. Lsa46 and Lsa77 also bind plasma fibronectin, and both adhesins are plasminogen (PLG-interacting proteins, capable of generating plasmin (PLA and as such, increase the proteolytic ability of leptospires. The proteins corresponding to Lsa46 and Lsa77 are present in virulent L. interrogans L1-130 and in saprophyte L. biflexa Patoc 1 strains, as detected by immunofluorescence. The adhesins are recognized by human leptospirosis serum samples at the onset and convalescent phases of the disease, suggesting that they are expressed during infection. Taken together, our data could offer valuable information to the understanding of leptospiral pathogenesis.

  7. Engineering Software Suite Validates System Design

    Science.gov (United States)

    2007-01-01

    EDAptive Computing Inc.'s (ECI) EDAstar engineering software tool suite, created to capture and validate system design requirements, was significantly funded by NASA's Ames Research Center through five Small Business Innovation Research (SBIR) contracts. These programs specifically developed Syscape, used to capture executable specifications of multi-disciplinary systems, and VectorGen, used to automatically generate tests to ensure system implementations meet specifications. According to the company, the VectorGen tests considerably reduce the time and effort required to validate implementation of components, thereby ensuring their safe and reliable operation. EDASHIELD, an additional product offering from ECI, can be used to diagnose, predict, and correct errors after a system has been deployed using EDASTAR -created models. Initial commercialization for EDASTAR included application by a large prime contractor in a military setting, and customers include various branches within the U.S. Department of Defense, industry giants like the Lockheed Martin Corporation, Science Applications International Corporation, and Ball Aerospace and Technologies Corporation, as well as NASA's Langley and Glenn Research Centers

  8. The Inelastic Instrument suite at the SNS

    International Nuclear Information System (INIS)

    Granroth, Garrett E; Abernathy, Douglas L; Ehlers, Georg; Hagen, Mark E; Herwig, Kenneth W; Mamontov, Eugene; Ohl, Michael E; Wildgruber, Christoph U

    2008-01-01

    The instruments in the extensive suite of spectrometers at the SNS are in various stages of installation and commissioning. The Back Scattering Spectrometer (BASIS) is installed and is in commissioning. It's near backscattering analyzer crystals provide the 3 eV resolution as expected. BASIS will enter the user program in the fall of 2007. The ARCS wide angular-range thermal to epithermal neutron spectrometer will come on line in the fall of 2007 followed shortly by the Cold Neutron Chopper Spectrometer. These two direct geometry instruments provide moderate resolution and the ability to trade resolution for flux. In addition both instruments have detector coverage out to 140o to provide a large Q range. The SEQUOIA spectrometer, complete in 2008, is the direct geometry instrument that will provide fine resolution in the thermal to epithermal range. The Spin-Echo spectrometer, to be completed on a similar time scale, will provide the finest energy resolution worldwide. The HYSPEC spectrometer, available no later than 2011, will provide polarized capabilities and optimized flux in the thermal energy range. Finally, the Vision chemical spectrometer will use crystal analyzers to study energy transfers into the epithermal range

  9. Automated Structure Solution with the PHENIX Suite

    Energy Technology Data Exchange (ETDEWEB)

    Zwart, Peter H.; Zwart, Peter H.; Afonine, Pavel; Grosse-Kunstleve, Ralf W.; Hung, Li-Wei; Ioerger, Tom R.; McCoy, A.J.; McKee, Eric; Moriarty, Nigel; Read, Randy J.; Sacchettini, James C.; Sauter, Nicholas K.; Storoni, L.C.; Terwilliger, Tomas C.; Adams, Paul D.

    2008-06-09

    Significant time and effort are often required to solve and complete a macromolecular crystal structure. The development of automated computational methods for the analysis, solution and completion of crystallographic structures has the potential to produce minimally biased models in a short time without the need for manual intervention. The PHENIX software suite is a highly automated system for macromolecular structure determination that can rapidly arrive at an initial partial model of a structure without significant human intervention, given moderate resolution and good quality data. This achievement has been made possible by the development of new algorithms for structure determination, maximum-likelihood molecular replacement (PHASER), heavy-atom search (HySS), template and pattern-based automated model-building (RESOLVE, TEXTAL), automated macromolecular refinement (phenix.refine), and iterative model-building, density modification and refinement that can operate at moderate resolution (RESOLVE, AutoBuild). These algorithms are based on a highly integrated and comprehensive set of crystallographic libraries that have been built and made available to the community. The algorithms are tightly linked and made easily accessible to users through the PHENIX Wizards and the PHENIX GUI.

  10. Automated structure solution with the PHENIX suite

    Energy Technology Data Exchange (ETDEWEB)

    Terwilliger, Thomas C [Los Alamos National Laboratory; Zwart, Peter H [LBNL; Afonine, Pavel V [LBNL; Grosse - Kunstleve, Ralf W [LBNL

    2008-01-01

    Significant time and effort are often required to solve and complete a macromolecular crystal structure. The development of automated computational methods for the analysis, solution, and completion of crystallographic structures has the potential to produce minimally biased models in a short time without the need for manual intervention. The PHENIX software suite is a highly automated system for macromolecular structure determination that can rapidly arrive at an initial partial model of a structure without significant human intervention, given moderate resolution, and good quality data. This achievement has been made possible by the development of new algorithms for structure determination, maximum-likelihood molecular replacement (PHASER), heavy-atom search (HySS), template- and pattern-based automated model-building (RESOLVE, TEXTAL), automated macromolecular refinement (phenix. refine), and iterative model-building, density modification and refinement that can operate at moderate resolution (RESOLVE, AutoBuild). These algorithms are based on a highly integrated and comprehensive set of crystallographic libraries that have been built and made available to the community. The algorithms are tightly linked and made easily accessible to users through the PHENIX Wizards and the PHENIX GUI.

  11. The Variable Vector Countermeasure Suit (V2Suit for Space Habitation and Exploration

    Directory of Open Access Journals (Sweden)

    Kevin R Duda

    2015-04-01

    Full Text Available The Variable Vector Countermeasure Suit (V2Suit for Space Habitation and Exploration is a novel system concept that provides a platform for integrating sensors and actuators with daily astronaut intravehicular activities to improve health and performance, while reducing the mass and volume of the physiologic adaptation countermeasure systems, as well as the required exercise time during long-duration space exploration missions. The V2Suit system leverages wearable kinematic monitoring technology and uses inertial measurement units (IMUs and control moment gyroscopes (CMGs within miniaturized modules placed on body segments to provide a viscous resistance during movements against a specified direction of down – initially as a countermeasure to the sensorimotor adaptation performance decrements that manifest themselves while living and working in microgravity and during gravitational transitions during long-duration spaceflight, including post-flight recovery and rehabilitation. Several aspects of the V2Suit system concept were explored and simulated prior to developing a brassboard prototype for technology demonstration. This included a system architecture for identifying the key components and their interconnects, initial identification of key human-system integration challenges, development of a simulation architecture for CMG selection and parameter sizing, and the detailed mechanical design and fabrication of a module. The brassboard prototype demonstrates closed-loop control from down initialization through CMG actuation, and provides a research platform for human performance evaluations to mitigate sensorimotor adaptation, as well as a tool for determining the performance requirements when used as a musculoskeletal deconditioning countermeasure. This type of countermeasure system also has Earth benefits, particularly in gait or movement stabilization and rehabilitation.

  12. The ESA's Space Trajectory Analysis software suite

    Science.gov (United States)

    Ortega, Guillermo

    The European Space Agency (ESA) initiated in 2005 an internal activity to develop an open source software suite involving university science departments and research institutions all over the world. This project is called the "Space Trajectory Analysis" or STA. This article describes the birth of STA and its present configuration. One of the STA aims is to promote the exchange of technical ideas, and raise knowledge and competence in the areas of applied mathematics, space engineering, and informatics at University level. Conceived as a research and education tool to support the analysis phase of a space mission, STA is able to visualize a wide range of space trajectories. These include among others ascent, re-entry, descent and landing trajectories, orbits around planets and moons, interplanetary trajectories, rendezvous trajectories, etc. The article explains that STA project is an original idea of the Technical Directorate of ESA. It was born in August 2005 to provide a framework in astrodynamics research at University level. As research and education software applicable to Academia, a number of Universities support this development by joining ESA in leading the development. ESA and Universities partnership are expressed in the STA Steering Board. Together with ESA, each University has a chair in the board whose tasks are develop, control, promote, maintain, and expand the software suite. The article describes that STA provides calculations in the fields of spacecraft tracking, attitude analysis, coverage and visibility analysis, orbit determination, position and velocity of solar system bodies, etc. STA implements the concept of "space scenario" composed of Solar system bodies, spacecraft, ground stations, pads, etc. It is able to propagate the orbit of a spacecraft where orbital propagators are included. STA is able to compute communication links between objects of a scenario (coverage, line of sight), and to represent the trajectory computations and

  13. OCAMS: The OSIRIS-REx Camera Suite

    Science.gov (United States)

    Rizk, B.; Drouet d'Aubigny, C.; Golish, D.; Fellows, C.; Merrill, C.; Smith, P.; Walker, M. S.; Hendershot, J. E.; Hancock, J.; Bailey, S. H.; DellaGiustina, D. N.; Lauretta, D. S.; Tanner, R.; Williams, M.; Harshman, K.; Fitzgibbon, M.; Verts, W.; Chen, J.; Connors, T.; Hamara, D.; Dowd, A.; Lowman, A.; Dubin, M.; Burt, R.; Whiteley, M.; Watson, M.; McMahon, T.; Ward, M.; Booher, D.; Read, M.; Williams, B.; Hunten, M.; Little, E.; Saltzman, T.; Alfred, D.; O'Dougherty, S.; Walthall, M.; Kenagy, K.; Peterson, S.; Crowther, B.; Perry, M. L.; See, C.; Selznick, S.; Sauve, C.; Beiser, M.; Black, W.; Pfisterer, R. N.; Lancaster, A.; Oliver, S.; Oquest, C.; Crowley, D.; Morgan, C.; Castle, C.; Dominguez, R.; Sullivan, M.

    2018-02-01

    The OSIRIS-REx Camera Suite (OCAMS) will acquire images essential to collecting a sample from the surface of Bennu. During proximity operations, these images will document the presence of satellites and plumes, record spin state, enable an accurate model of the asteroid's shape, and identify any surface hazards. They will confirm the presence of sampleable regolith on the surface, observe the sampling event itself, and image the sample head in order to verify its readiness to be stowed. They will document Bennu's history as an example of early solar system material, as a microgravity body with a planetesimal size-scale, and as a carbonaceous object. OCAMS is fitted with three cameras. The MapCam will record color images of Bennu as a point source on approach to the asteroid in order to connect Bennu's ground-based point-source observational record to later higher-resolution surface spectral imaging. The SamCam will document the sample site before, during, and after it is disturbed by the sample mechanism. The PolyCam, using its focus mechanism, will observe the sample site at sub-centimeter resolutions, revealing surface texture and morphology. While their imaging requirements divide naturally between the three cameras, they preserve a strong degree of functional overlap. OCAMS and the other spacecraft instruments will allow the OSIRIS-REx mission to collect a sample from a microgravity body on the same visit during which it was first optically acquired from long range, a useful capability as humanity reaches out to explore near-Earth, Main-Belt and Jupiter Trojan asteroids.

  14. Vehicle-network defensive aids suite

    Science.gov (United States)

    Rapanotti, John

    2005-05-01

    Defensive Aids Suites (DAS) developed for vehicles can be extended to the vehicle network level. The vehicle network, typically comprising four platoon vehicles, will benefit from improved communications and automation based on low latency response to threats from a flexible, dynamic, self-healing network environment. Improved DAS performance and reliability relies on four complementary sensor technologies including: acoustics, visible and infrared optics, laser detection and radar. Long-range passive threat detection and avoidance is based on dual-purpose optics, primarily designed for manoeuvring, targeting and surveillance, combined with dazzling, obscuration and countermanoeuvres. Short-range active armour is based on search and track radar and intercepting grenades to defeat the threat. Acoustic threat detection increases the overall robustness of the DAS and extends the detection range to include small calibers. Finally, detection of active targeting systems is carried out with laser and radar warning receivers. Synthetic scene generation will provide the integrated environment needed to investigate, develop and validate these new capabilities. Computer generated imagery, based on validated models and an acceptable set of benchmark vignettes, can be used to investigate and develop fieldable sensors driven by real-time algorithms and countermeasure strategies. The synthetic scene environment will be suitable for sensor and countermeasure development in hardware-in-the-loop simulation. The research effort focuses on two key technical areas: a) computing aspects of the synthetic scene generation and b) and development of adapted models and databases. OneSAF is being developed for research and development, in addition to the original requirement of Simulation and Modelling for Acquisition, Rehearsal, Requirements and Training (SMARRT), and is becoming useful as a means for transferring technology to other users, researchers and contractors. This procedure

  15. Advanced Sensor Platform to Evaluate Manloads For Exploration Suit Architectures

    Science.gov (United States)

    McFarland, Shane; Pierce, Gregory

    2016-01-01

    Space suit manloads are defined as the outer bounds of force that the human occupant of a suit is able to exert onto the suit during motion. They are defined on a suit-component basis as a unit of maximum force that the suit component in question must withstand without failure. Existing legacy manloads requirements are specific to the suit architecture of the EMU and were developed in an iterative fashion; however, future exploration needs dictate a new suit architecture with bearings, load paths, and entry capability not previously used in any flight suit. No capability currently exists to easily evaluate manloads imparted by a suited occupant, which would be required to develop requirements for a flight-rated design. However, sensor technology has now progressed to the point where an easily-deployable, repeatable and flexible manloads measuring technique could be developed leveraging recent advances in sensor technology. INNOVATION: This development positively impacts schedule, cost and safety risk associated with new suit exploration architectures. For a final flight design, a comprehensive and accurate man loads requirements set must be communicated to the contractor; failing that, a suit design which does not meet necessary manloads limits is prone to failure during testing or worse, during an EVA, which could cause catastrophic failure of the pressure garment posing risk to the crew. This work facilitates a viable means of developing manloads requirements using a range of human sizes & strengths. OUTCOME / RESULTS: Performed sensor market research. Highlighted three viable options (primary, secondary, and flexible packaging option). Designed/fabricated custom bracket to evaluate primary option on a single suit axial. Manned suited manload testing completed and general approach verified.

  16. Innovative technology summary report: Sealed-seam sack suits

    International Nuclear Information System (INIS)

    1998-09-01

    Sealed-seam sack suits are an improved/innovative safety and industrial hygiene technology designed to protect workers from dermal exposure to contamination. Most of these disposable, synthetic-fabric suits are more protective than cotton suits, and are also water-resistant and gas permeable. Some fabrics provide a filter to aerosols, which is important to protection against contamination, while allowing air to pass, increasing comfort level of workers. It is easier to detect body-moisture breakthrough with the disposable suits than with cotton, which is also important to protecting workers from contamination. These suits present a safe and cost-effective (6% to 17% less expensive than the baseline) alternative to traditional protective clothing. This report covers the period from October 1996 to August 1997. During that time, sealed-seam sack suits were demonstrated during daily activities under normal working conditions at the C Reactor and under environmentally controlled conditions at the Los Alamos National Laboratory (LANL)

  17. Alterations in MAST suit pressure with changes in ambient temperature.

    Science.gov (United States)

    Sanders, A B; Meislin, H W; Daub, E

    1983-01-01

    A study was undertaken to test the hypothesis that change in ambient air temperature has an effect on MAST suit pressure according to the ideal gas law. Two different MAST suits were tested on Resusci-Annie dummies. The MAST suits were applied in a cold room at 4.4 degrees C and warmed to 44 degrees C. Positive linear correlations were found in nine trials, but the two suits differed in their rate of increase in pressure. Three trials using humans were conducted showing increased pressure with temperature but at a lesser rate than with dummies. A correlation of 0.5 to 1.0 mm Hg increase in MAST suit pressure for each 1.0 degrees C increase in ambient temperature was found. Implications are discussed for the use of the MAST suit in environmental conditions where the temperature changes.

  18. Problem of Office Suite Training at the University

    Directory of Open Access Journals (Sweden)

    Natalia A. Nastashchuk

    2013-01-01

    Full Text Available Te paper considers the problem of office suite applications training, caused by a rapid change of their versions, variety of software developers and a rapid development of software and hardware platforms. The content of office suite applications training, based on the system of office suite notions, its basic functional and standards of information technologies development (OpenDocument Format Standard, ISO 26300-200Х is presented.

  19. Miniature Flexible Humidity Sensitive Patches for Space Suits, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Advanced space suit technologies demand improved, simplified, long-life regenerative sensing technologies, including humidity sensors, that exceed the performance of...

  20. Leveraging Active Knit Technologies for Aerospace Pressure Suit Applications

    Data.gov (United States)

    National Aeronautics and Space Administration — Anti-Gravity Suits (AGS) are garments used in astronautics to prevent crew from experiencing orthostatic intolerance (OI) and consequential blackouts while...

  1. Chloroform-Methanol Residue of Coxiella burnetii Markedly Potentiated the Specific Immunoprotection Elicited by a Recombinant Protein Fragment rOmpB-4 Derived from Outer Membrane Protein B of Rickettsia rickettsii in C3H/HeN Mice.

    Directory of Open Access Journals (Sweden)

    Wenping Gong

    Full Text Available The obligate intracellular bacteria, Rickettsia rickettsii and Coxiella burnetii, are the potential agents of bio-warfare/bio-terrorism. Here C3H/HeN mice were immunized with a recombinant protein fragment rOmp-4 derived from outer membrane protein B, a major protective antigen of R. rickettsii, combined with chloroform-methanol residue (CMR extracted from phase I C. burnetii organisms, a safer Q fever vaccine. These immunized mice had significantly higher levels of IgG1 and IgG2a to rOmpB-4 and interferon-γ (IFN-γ and tumor necrosis factor-α (TNF-α, two crucial cytokines in resisting intracellular bacterial infection, as well as significantly lower rickettsial loads and slighter pathological lesions in organs after challenge with R. rickettsii, compared with mice immunized with rOmpB-4 or CMR alone. Additionally, after challenge with C. burnetii, the coxiella loads in the organs of these mice were significantly lower than those of mice immunized with rOmpB-4 alone. Our results prove that CMR could markedly potentiate enhance the rOmpB-4-specific immunoprotection by promoting specific and non-specific immunoresponses and the immunization with the protective antigen of R. rickettsii combined with CMR of C. burnetii could confer effective protection against infection of R. rickettsii or C. burnetii.

  2. Use MACES IVA Suit for EVA Mobility Evaluations

    Science.gov (United States)

    Watson, Richard D.

    2014-01-01

    The use of an Intra-Vehicular Activity (IVA) suit for a spacewalk or Extra-Vehicular Activity (EVA) was evaluated for mobility and usability in the Neutral Buoyancy Lab (NBL) environment. The Space Shuttle Advanced Crew Escape Suit (ACES) has been modified (MACES) to integrate with the Orion spacecraft. The first several missions of the Orion MPCV spacecraft will not have mass available to carry an EVA specific suit so any EVA required will have to be performed by the MACES. Since the MACES was not designed with EVA in mind, it was unknown what mobility the suit would be able to provide for an EVA or if a person could perform useful tasks for an extended time inside the pressurized suit. The suit was evaluated in multiple NBL runs by a variety of subjects including crewmembers with significant EVA experience. Various functional mobility tasks performed included: translation, body positioning, carrying tools, body stabilization, equipment handling, and use of tools. Hardware configurations included with and without TMG, suit with IVA gloves and suit with EVA gloves. Most tasks were completed on ISS mockups with existing EVA tools. Some limited tasks were completed with prototype tools on a simulated rocky surface. Major findings include: demonstration of the ability to weigh-out the suit, understanding the need to have subjects perform multiple runs prior to getting feedback, determination of critical sizing factors, and need for adjustment of suit work envelop. The early testing has demonstrated the feasibility of EVA's limited duration and limited scope. Further testing is required with more flight like tasking and constraints to validate these early results. If the suit is used for EVA, it will require mission specific modifications for umbilical management or PLSS integration, safety tether attachment, and tool interfaces. These evaluations are continuing through calendar year 2014.

  3. Morphing: A Novel Approach to Astronaut Suit Sizing

    Science.gov (United States)

    Margerum, Sarah; Clowers, Kurt; Rajulu, Sudhakar

    2006-01-01

    The fitting of a spacesuit to an astronaut is an iterative process consisting of two parts. The first uses anthropometric data to provide an approximation of the suit components that will fit the astronaut. The second part is the subjective fitting, where small adjustments are made based on the astronaut s preference. By providing a better approximation of the correct suit components, the entire fit process time can be reduced significantly. The goals of this project are twofold: (1) To evaluate the effectiveness of the existing sizing algorithm for the Mark III Hybrid suit and (2) to determine what additional components are needed in order to provide adequate sizing for the existing astronaut population. A single subject was scanned using a 3D whole-body scanner (VITUS 3D) in the Mark III suit in eight different poses and four subjects in minimal clothing were also scanned in similar poses. The 3D external body scans of the suit and the subject are overlaid and visually aligned in a customized MATLAB program. The suit components were contracted or expanded linearly along the subjects limbs to match the subjects segmental lengths. Two independent measures were obtained from the morphing program on four subjects and compared with the existing sizing information. Two of the four subjects were in correspondence with the sizing algorithm and morphing results. The morphing outcome for a third subject, incompatible with the suit, suggested that an additional arm element at least 6 inches smaller than the existing smallest suit component would need to be acquired. The morphing result of the fourth subject, deemed incompatible with the suit using the sizing algorithm, indicated a different suit configuration which would be compatible. This configuration matched with the existing suit fit check data.

  4. Exploration Space Suit Architecture and Destination Environmental-Based Technology Development

    Science.gov (United States)

    Hill, Terry R.; McFarland, Shane M.; Korona, F. Adam

    2013-01-01

    This paper continues forward where EVA Space Suit Architecture: Low Earth Orbit Vs. Moon Vs. Mars left off in the development of a space suit architecture that is modular in design and could be reconfigured prior to launch or during any given mission depending on the tasks or destination. This space suit system architecture and technologies required based on human exploration (EVA) destinations will be discussed, and how these systems should evolve to meet the future exploration EVA needs of the US human space flight program. A series of exercises and analyses provided a strong indication that the Constellation Program space suit architecture, with its maximum reuse of technology and functionality across a range of mission profiles and destinations, is postured to provide a viable solution for future space exploration missions. The destination environmental analysis demonstrates that the modular architecture approach could provide the lowest mass and mission cost for the protection of the crew, given any human mission outside of low-Earth orbit. Additionally, some of the high-level trades presented here provide a review of the environmental and nonenvironmental design drivers that will become increasingly important as humans venture farther from Earth. The presentation of destination environmental data demonstrates a logical clustering of destination design environments that allows a focused approach to technology prioritization, development, and design that will maximize the return on investment, largely independent of any particular design reference mission.

  5. The BRITNeY Suite: A Platfor for Experiments

    DEFF Research Database (Denmark)

    Westergaard, Michael

    2006-01-01

    This paper describes a platform, the BRITNeY Suite, for experimenting with Coloured Petri nets. The BRITNeY Suite provides access to data-structures and a simulator for Coloured Petri nets via a powerful scripting language and plug-in-mechanism, thereby making it easy to perform customized...

  6. Planetary Suit Hip Bearing Model for Predicting Design vs. Performance

    Science.gov (United States)

    Cowley, Matthew S.; Margerum, Sarah; Harvil, Lauren; Rajulu, Sudhakar

    2011-01-01

    Designing a planetary suit is very complex and often requires difficult trade-offs between performance, cost, mass, and system complexity. In order to verifying that new suit designs meet requirements, full prototypes must eventually be built and tested with human subjects. Using computer models early in the design phase of new hardware development can be advantageous, allowing virtual prototyping to take place. Having easily modifiable models of the suit hard sections may reduce the time it takes to make changes to the hardware designs and then to understand their impact on suit and human performance. A virtual design environment gives designers the ability to think outside the box and exhaust design possibilities before building and testing physical prototypes with human subjects. Reductions in prototyping and testing may eventually reduce development costs. This study is an attempt to develop computer models of the hard components of the suit with known physical characteristics, supplemented with human subject performance data. Objectives: The primary objective was to develop an articulating solid model of the Mark III hip bearings to be used for evaluating suit design performance of the hip joint. Methods: Solid models of a planetary prototype (Mark III) suit s hip bearings and brief section were reverse-engineered from the prototype. The performance of the models was then compared by evaluating the mobility performance differences between the nominal hardware configuration and hardware modifications. This was accomplished by gathering data from specific suited tasks. Subjects performed maximum flexion and abduction tasks while in a nominal suit bearing configuration and in three off-nominal configurations. Performance data for the hip were recorded using state-of-the-art motion capture technology. Results: The results demonstrate that solid models of planetary suit hard segments for use as a performance design tool is feasible. From a general trend perspective

  7. Correction factors for assessing immersion suits under harsh conditions.

    Science.gov (United States)

    Power, Jonathan; Tikuisis, Peter; Ré, António Simões; Barwood, Martin; Tipton, Michael

    2016-03-01

    Many immersion suit standards require testing of thermal protective properties in calm, circulating water while these suits are typically used in harsher environments where they often underperform. Yet it can be expensive and logistically challenging to test immersion suits in realistic conditions. The goal of this work was to develop a set of correction factors that would allow suits to be tested in calm water yet ensure they will offer sufficient protection in harsher conditions. Two immersion studies, one dry and the other with 500 mL of water within the suit, were conducted in wind and waves to measure the change in suit insulation. In both studies, wind and waves resulted in a significantly lower immersed insulation value compared to calm water. The minimum required thermal insulation for maintaining heat balance can be calculated for a given mean skin temperature, metabolic heat production, and water temperature. Combining the physiological limits of sustainable cold water immersion and actual suit insulation, correction factors can be deduced for harsh conditions compared to calm. The minimum in-situ suit insulation to maintain thermal balance is 1.553-0.0624·TW + 0.00018·TW(2) for a dry calm condition. Multiplicative correction factors to the above equation are 1.37, 1.25, and 1.72 for wind + waves, 500 mL suit wetness, and both combined, respectively. Calm water certification tests of suit insulation should meet or exceed the minimum in-situ requirements to maintain thermal balance, and correction factors should be applied for a more realistic determination of minimum insulation for harsh conditions. Crown Copyright © 2015. Published by Elsevier Ltd. All rights reserved.

  8. [Eukaryotic expression of Leptospira interrogans lipL32/1-ompL1/1 fusion gene encoding genus-specific protein antigens and the immunoreactivity of expression products].

    Science.gov (United States)

    Yan, Jie; Zhao, Shou-feng; Mao, Ya-fei; Ruan, Ping; Luo, Yi-hui; Li, Shu-ping; Li, Li-wei

    2005-01-01

    To construct the eukaryotic expression system of L.interrogans lipL32/1-ompL1/1 fusion gene and to identify the immunoreactivity of expression products. PCR with linking primer was used to construct the fusion gene lipL32/1-ompL1/1. The P.pastoris eukaryotic expression system of the fusion gene, pPIC9K-lipL32/1-ompL1/1-P. pastorisGS115, was constructed after the fusion gene was cloned and sequenced. Colony with phenotype His(+)Mut(+) was isolated by using MD and MM plates and His(+) Mut(+) transformant with high resistance to G418 was screened out by using YPD plate. Using lysate of His(+) Mut(+) colony with high copies of the target gene digested with yeast lyase as the template and 5'AOX1 and 3'AOX1 as the primers, the target fusion gene in chromosome DNA of the constructed P. pastoris engineering strain was detected by PCR. Methanol in BMMY medium was used to induce the target recombinant protein rLipL32/1-rOmpL1/1 expression. rLipL32/1-rOmpL1/1 in the medium supernatant was extracted by using ammonium sulfate precipitation and Ni-NTA affinity chromatography. Output and immunoreactivity of rLipL32/1-rOmpL1/1 were measured by SDS-PAGE and Western blot methods, respectively. Amplification fragments of the obtained fusion gene lipL32/1-ompL1/1 was 1794 bp in size. The homogeneity of nucleotide and putative amino acid sequences of the fusion gene were as high as 99.94 % and 100 %, respectively, compared with the sequences of original lipL32/1 and ompL1/1 genotypes. The constructed eukaryotic expression system was able to secrete rLipL32/1-rOmpL1/1 with an output of 10 % of the total proteins in the supernatant, which located the expected position after SDS-PAGE. The rabbit anti-rLipL32/1 and anti-rOmpL1/1 sera could combine the expressed rLipL32/1-rOmpL1/1. An eukaryotic expression system with high efficiency in P.pastoris of L.interrogans lipL32/1-ompL1/1 fusion gene was successfully constructed in this study. The expressed fusion protein shows specific

  9. Development of the DL/H-1 full pressure suit for private spaceflight

    Science.gov (United States)

    León, Pablo de; Harris, Gary L.

    2010-06-01

    The objective of this paper is to detail the need for full pressure suits to protect spaceflight participants during the experimental phases of flight testing of new space vehicles. It also details the objectives, historical background, basis for design, problems encountered by the designers and final development of the DL/H-1 full pressure suit. It will include justification for its use and results of the initial tests in the high altitude chamber and spacecraft simulator at the J.D. Odegard School of Aerospace Sciences at the University of North Dakota. For the test flights of early commercial space vehicles and tourist suborbital spacecrafts, emergency protection from the rarified air of the upper atmosphere and the vacuum of low Earth orbit almost certainly will be a requirement. Suborbital vehicles could be operating in "space equivalent conditions" for as long as 30 min to as much as several hours. In the case of cabin pressure loss, without personal protection, catastrophic loss of crew and vehicle could result. This paper explains the different steps taken by the authors who designed and built a preflight hardware pressure suit that can meet the physiological and comfort requirements of the tourist suborbital industry and the early commercial private spaceflight community. The suborbital tourist and commercial spaceflight industry have unique problems confronting the pressure suit builder such as unpressurized comfort, reasonable expense, unique sizing of the general population, decompression complications of persons not fitting a past military physiology profile and equipment weight issues. In addition, the lack of a certifying agency or guidance from international or national aviation authorities has created the opportunity for the emerging civilian pressure suit industry to create a new safety standard by which it can regulate itself in the same way the recreational SCUBA diving industry has since the late 1950s.

  10. Hybrid Enhanced Epidermal SpaceSuit Design Approaches

    Science.gov (United States)

    Jessup, Joseph M.

    A Space suit that does not rely on gas pressurization is a multi-faceted problem that requires major stability controls to be incorporated during design and construction. The concept of Hybrid Epidermal Enhancement space suit integrates evolved human anthropomorphic and physiological adaptations into its functionality, using commercially available bio-medical technologies to address shortcomings of conventional gas pressure suits, and the impracticalities of MCP suits. The prototype HEE Space Suit explored integumentary homeostasis, thermal control and mobility using advanced bio-medical materials technology and construction concepts. The goal was a space suit that functions as an enhanced, multi-functional bio-mimic of the human epidermal layer that works in attunement with the wearer rather than as a separate system. In addressing human physiological requirements for design and construction of the HEE suit, testing regimes were devised and integrated into the prototype which was then subject to a series of detailed tests using both anatomical reproduction methods and human subject.

  11. General Method to Determine the Flux of Charged Molecules through Nanopores Applied to β-Lactamase Inhibitors and OmpF.

    Science.gov (United States)

    Ghai, Ishan; Pira, Alessandro; Scorciapino, Mariano Andrea; Bodrenko, Igor; Benier, Lorraine; Ceccarelli, Matteo; Winterhalter, Mathias; Wagner, Richard

    2017-03-16

    A major challenge in the discovery of the new antibiotics against Gram-negative bacteria is to achieve sufficiently fast permeation in order to avoid high doses causing toxic side effects. So far, suitable assays for quantifying the uptake of charged antibiotics into bacteria are lacking. We apply an electrophysiological zero-current assay using concentration gradients of β-lactamase inhibitors combined with single-channel conductance to quantify their flux rates through OmpF. Molecular dynamic simulations provide in addition details on the interactions between the nanopore wall and the charged solutes. In particular, the interaction barrier for three β-lactamase inhibitors is surprisingly as low as 3-5 kcal/mol and only slightly above the diffusion barrier of ions such as chloride. Within our macroscopic constant field model, we determine that at a zero-membrane potential a concentration gradient of 10 μM of avibactam, sulbactam, or tazobactam can create flux rates of roughly 620 molecules/s per OmpF trimer.

  12. Vaccination with recombinant L7/L12-truncated Omp31 protein induces protection against Brucella infection in BALB/c mice.

    Science.gov (United States)

    Golshani, Maryam; Rafati, Sima; Dashti, Amir; Gholami, Elham; Siadat, Seyed Davar; Oloomi, Mana; Jafari, Anis; Bouzari, Saeid

    2015-06-01

    Brucellosis is the most common bacterial zoonotic disease worldwide and no vaccine is available for the prevention of human brucellosis. In humans, brucellosis is mostly caused by Brucella melitensis and Brucella abortus. The Outer membrane protein 31 (Omp31) and L7/L12 are immunodominant and protective antigens conserved in human Brucella pathogens. In the present study, we evaluated the humoral and cellular immune responses induced by a fusion protein designed based on the Truncated form of Omp31 (TOmp31) and L7-L12 antigens. Vaccination of BALB/c mice with the recombinant fusion protein (rL7/L12-TOmp31) provided the significant protection level against B. melitensis and B. abortus challenge. Moreover, rL7/L12-TOmp31 elicited a strong specific IgG response (higher IgG2a titers) and significant IFN-γ/IL2 production and T-cell proliferation was also observed. The T helper1 (Th1) oriented response persisted for 12 weeks after the first immunization. The rL7/L12-TOmp31 could be a new potential antigen candidate for the development of a subunit vaccine against B. melitensis and B. abortus. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. A Secure Communication Suite for Underwater Acoustic Sensor Networks

    Directory of Open Access Journals (Sweden)

    Angelica Lo Duca

    2012-11-01

    Full Text Available In this paper we describe a security suite for Underwater Acoustic Sensor Networks comprising both fixed and mobile nodes. The security suite is composed of a secure routing protocol and a set of cryptographic primitives aimed at protecting the confidentiality and the integrity of underwater communication while taking into account the unique characteristics and constraints of the acoustic channel. By means of experiments and simulations based on real data, we show that the suite is suitable for an underwater networking environment as it introduces limited, and sometimes negligible, communication and power consumption overhead.

  14. Development on smart suit for dairy work assistance.

    Science.gov (United States)

    Nara, Hiroyuki; Kusaka, Takashi; Tanaka, Takayuki; Yamagishi, Takayuki; Ogura, Shotaroh

    2013-01-01

    Our purpose in this study is to achieve an independent life and a social involvement for the elderly using KEIROKA Technology(fatigue-reduction) which makes it possible to improve the quality of chores and occupations by removing excessive strain and tiredness. The authors have developed power assist suits named "smart suit". The authors have evaluated the effect that the purpose of dairy work assistance, to measure EMG of the worker, compared to the potential of the surface of the non-wearing and wearing "smart suit".

  15. Inertial motion capture system for biomechanical analysis in pressure suits

    Science.gov (United States)

    Di Capua, Massimiliano

    A non-invasive system has been developed at the University of Maryland Space System Laboratory with the goal of providing a new capability for quantifying the motion of the human inside a space suit. Based on an array of six microprocessors and eighteen microelectromechanical (MEMS) inertial measurement units (IMUs), the Body Pose Measurement System (BPMS) allows the monitoring of the kinematics of the suit occupant in an unobtrusive, self-contained, lightweight and compact fashion, without requiring any external equipment such as those necessary with modern optical motion capture systems. BPMS measures and stores the accelerations, angular rates and magnetic fields acting upon each IMU, which are mounted on the head, torso, and each segment of each limb. In order to convert the raw data into a more useful form, such as a set of body segment angles quantifying pose and motion, a series of geometrical models and a non-linear complimentary filter were implemented. The first portion of this works focuses on assessing system performance, which was measured by comparing the BPMS filtered data against rigid body angles measured through an external VICON optical motion capture system. This type of system is the industry standard, and is used here for independent measurement of body pose angles. By comparing the two sets of data, performance metrics such as BPMS system operational conditions, accuracy, and drift were evaluated and correlated against VICON data. After the system and models were verified and their capabilities and limitations assessed, a series of pressure suit evaluations were conducted. Three different pressure suits were used to identify the relationship between usable range of motion and internal suit pressure. In addition to addressing range of motion, a series of exploration tasks were also performed, recorded, and analysed in order to identify different motion patterns and trajectories as suit pressure is increased and overall suit mobility is reduced

  16. Suite of Activity-Based Probes for Cellulose-Degrading Enzymes

    Energy Technology Data Exchange (ETDEWEB)

    Chauvigne-Hines, Lacie M.; Anderson, Lindsey N.; Weaver, Holly M.; Brown, Joseph N.; Koech, Phillip K.; Nicora, Carrie D.; Hofstad, Beth A.; Smith, Richard D.; Wilkins, Michael J.; Callister, Stephen J.; Wright, Aaron T.

    2012-12-19

    Microbial glycoside hydrolases play a dominant role in the biochemical conversion of cellulosic biomass to high-value biofuels. Anaerobic cellulolytic bacteria are capable of producing multicomplex catalytic subunits containing cell-adherent cellulases, hemicellulases, xylanases, and other glycoside hydrolases to facilitate the degradation of highly recalcitrant cellulose and other related plant cell wall polysaccharides. Clostridium thermocellum is a cellulosome producing bacterium that couples rapid reproduction rates to highly efficient degradation of crystalline cellulose. Herein, we have developed and applied a suite of difluoromethylphenyl aglycone, N-halogenated glycosylamine, and 2-deoxy-2-fluoroglycoside activity-based protein profiling (ABPP) probes to the direct labeling of the C. thermocellum cellulosomal secretome. These activity-based probes (ABPs) were synthesized with alkynes to harness the utility and multimodal possibilities of click chemistry, and to increase enzyme active site inclusion for LC-MS analysis. We directly analyzed ABP-labeled and unlabeled global MS data, revealing ABP selectivity for glycoside hydrolase (GH) enzymes in addition to a large collection of integral cellulosome-containing proteins. By identifying reactivity and selectivity profiles for each ABP, we demonstrate our ability to widely profile the functional cellulose degrading machinery of the bacterium. Derivatization of the ABPs, including reactive groups, acetylation of the glycoside binding groups, and mono- and disaccharide binding groups, resulted in considerable variability in protein labeling. Our probe suite is applicable to aerobic and anaerobic cellulose degrading systems, and facilitates a greater understanding of the organismal role associated within biofuel development.

  17. Improvements to the APBS biomolecular solvation software suite: Improvements to the APBS Software Suite

    Energy Technology Data Exchange (ETDEWEB)

    Jurrus, Elizabeth [Pacific Northwest National Laboratory, Richland Washington; Engel, Dave [Pacific Northwest National Laboratory, Richland Washington; Star, Keith [Pacific Northwest National Laboratory, Richland Washington; Monson, Kyle [Pacific Northwest National Laboratory, Richland Washington; Brandi, Juan [Pacific Northwest National Laboratory, Richland Washington; Felberg, Lisa E. [University of California, Berkeley California; Brookes, David H. [University of California, Berkeley California; Wilson, Leighton [University of Michigan, Ann Arbor Michigan; Chen, Jiahui [Southern Methodist University, Dallas Texas; Liles, Karina [Pacific Northwest National Laboratory, Richland Washington; Chun, Minju [Pacific Northwest National Laboratory, Richland Washington; Li, Peter [Pacific Northwest National Laboratory, Richland Washington; Gohara, David W. [St. Louis University, St. Louis Missouri; Dolinsky, Todd [FoodLogiQ, Durham North Carolina; Konecny, Robert [University of California San Diego, San Diego California; Koes, David R. [University of Pittsburgh, Pittsburgh Pennsylvania; Nielsen, Jens Erik [Protein Engineering, Novozymes A/S, Copenhagen Denmark; Head-Gordon, Teresa [University of California, Berkeley California; Geng, Weihua [Southern Methodist University, Dallas Texas; Krasny, Robert [University of Michigan, Ann Arbor Michigan; Wei, Guo-Wei [Michigan State University, East Lansing Michigan; Holst, Michael J. [University of California San Diego, San Diego California; McCammon, J. Andrew [University of California San Diego, San Diego California; Baker, Nathan A. [Pacific Northwest National Laboratory, Richland Washington; Brown University, Providence Rhode Island

    2017-10-24

    The Adaptive Poisson-Boltzmann Solver (APBS) software was developed to solve the equations of continuum electrostatics for large biomolecular assemblages that has provided impact in the study of a broad range of chemical, biological, and biomedical applications. APBS addresses three key technology challenges for understanding solvation and electrostatics in biomedical applications: accurate and efficient models for biomolecular solvation and electrostatics, robust and scalable software for applying those theories to biomolecular systems, and mechanisms for sharing and analyzing biomolecular electrostatics data in the scientific community. To address new research applications and advancing computational capabilities, we have continually updated APBS and its suite of accompanying software since its release in 2001. In this manuscript, we discuss the models and capabilities that have recently been implemented within the APBS software package including: a Poisson-Boltzmann analytical and a semi-analytical solver, an optimized boundary element solver, a geometry-based geometric flow solvation model, a graph theory based algorithm for determining pKa values, and an improved web-based visualization tool for viewing electrostatics.

  18. CAMEO (Computer-Aided Management of Emergency Operations) Software Suite

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — CAMEO is the umbrella name for a system of software applications used widely to plan for and respond to chemical emergencies. All of the programs in the suite work...

  19. Segane saksa-eesti kirjakeel ja eesti lauluraamat / Gustav Suits

    Index Scriptorium Estoniae

    Suits, Gustav, 1883-1956

    1999-01-01

    Varem ilmunud: Suits, Gustav. Eesti kirjanduslugu I. Lund : Eesti Kirjanike Kooperatiiv, 1953. Heinrich Stahli käsiraamatu Hand-, Hausz- und Kirchenbuch (1654-1656) osana ilmunud lauluraamatust Neu Ehstnisches Gesangbuch (1656)

  20. Touring the Tomato: A Suite of Chemistry Laboratory Experiments

    Science.gov (United States)

    Sarkar, Sayantani; Chatterjee, Subhasish; Medina, Nancy; Stark, Ruth E.

    2013-01-01

    An eight-session interdisciplinary laboratory curriculum has been designed using a suite of analytical chemistry techniques to study biomaterials derived from an inexpensive source such as the tomato fruit. A logical

  1. Arensky. Silhouettes (Suite N 2), Op. 23 / Jonathan Swain

    Index Scriptorium Estoniae

    Swain, Jonathan

    1991-01-01

    Uuest heliplaadist "Arensky. Silhouettes (Suite N 2), Op. 23. Scrjabin. Symphony N 3 in C minor, Op. 43 "Le divin poeme". Danish National Radio Symphony Orchestra. Neeme Järvi. Chandos cassette ABTD 1509; CD CHAN 8898 (66 minutes)

  2. 33 CFR 144.20-5 - Exposure suits.

    Science.gov (United States)

    2010-07-01

    ... light that is approved under 46 CFR 161.012. Each light must be securely attached to the front shoulder... lanyard coiled and stopped off. (f) No stowage container for exposure suits may be capable of being locked...

  3. EVA Physiology and Medical Considerations Working in the Suit

    Science.gov (United States)

    Parazynski, Scott

    2012-01-01

    This "EVA Physiology and Medical Considerations Working in the Suit" presentation covers several topics related to the medical implications and physiological effects of suited operations in space from the perspective of a physician with considerable first-hand Extravehicular Activity (EVA) experience. Key themes include EVA physiology working in a pressure suit in the vacuum of space, basic EVA life support and work support, Thermal Protection System (TPS) inspections and repairs, and discussions of the physical challenges of an EVA. Parazynski covers the common injuries and significant risks during EVAs, as well as physical training required to prepare for EVAs. He also shares overall suit physiological and medical knowledge with the next generation of Extravehicular Mobility Unit (EMU) system designers.

  4. Advanced Gas Sensing Technology for Space Suits, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Advanced space suits require lightweight, low-power, durable sensors for monitoring critical life support materials. No current compact sensors have the tolerance...

  5. Virtual Suit Fit Assessment Using Body Shape Model

    Data.gov (United States)

    National Aeronautics and Space Administration — Shoulder injury is one of the most serious risks for crewmembers in long-duration spaceflight. While suboptimal suit fit and contact pressures between the shoulder...

  6. Tchaikovsky, P.: Orchestral Suite no. 3 op. 55 / Terry Williams

    Index Scriptorium Estoniae

    Williams, Terry

    1996-01-01

    Uuest heliplaadist "Tchaikovsky, P.: Orchestral Suite no. 3 op. 55. Francesca di Rimini op. 32. Detroit Symphony Orchestra, Neeme Järvi". Chandos CHAN 9 419, distribution Media 7 (CD: 160F). TT: 1h 09'20"

  7. Prokofiev: War and Peace - Symphonic Suite (arr. Palmer) / Ivan March

    Index Scriptorium Estoniae

    March, Ivan

    1993-01-01

    Uuest heliplaadist "Prokofiev: War and Peace - Symphonic Suite (arr. Palmer), Summer Night, Op. 123. Russian Overture, Op. 72. Philharmonia Orchestra / Neeme Järvi. Chandos ABTD 1598 CHAN9096 (64 minutes:DDD) Igor - Polovtsian Dances

  8. Prokofiev: Romeo and Juliet - Suite N1 / Ivan March

    Index Scriptorium Estoniae

    March, Ivan

    1990-01-01

    Uuest heliplaadist "Prokofiev: Romeo and Juliet - Suite N1, Op.64b, N2, Op.64c. Philharmonia Orchestra, Barry Wordsworth" Collins Classics cassette 1116-4. CD. Võrreldud Neeme Järvi plaadistustega 1116-2

  9. Nonventing Thermal and Humidity Control for EVA Suits, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Future manned space exploration missions will require space suits with capabilities beyond the current state of the art. Portable Life Support Systems for these...

  10. U.S. Climate Normals Product Suite (1981-2010)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA Climate Normals are a large suite of data products that provide users with many tools to understand typical climate conditions for thousands of locations...

  11. Assuring Condition and Inventory Accountability of Chemical Protective Suits

    National Research Council Canada - National Science Library

    2000-01-01

    .... As part of the Defense Logistics Agency's efforts to consolidate depot operations and improve inventory accuracy, chemical protective suits were transferred to the Defense Depot, Albany, Georgia, during FY 1991.

  12. Corrections of the NIST Statistical Test Suite for Randomness

    OpenAIRE

    Kim, Song-Ju; Umeno, Ken; Hasegawa, Akio

    2004-01-01

    It is well known that the NIST statistical test suite was used for the evaluation of AES candidate algorithms. We have found that the test setting of Discrete Fourier Transform test and Lempel-Ziv test of this test suite are wrong. We give four corrections of mistakes in the test settings. This suggests that re-evaluation of the test results should be needed.

  13. Cosmonaut Sergei Krikalev receives assistance from suit technician

    Science.gov (United States)

    1994-01-01

    Sergei Krikalev, alternative mission specialist for STS-63, gets help from Dawn Mays, a Boeing suit technician. The cosmonaut was about to participate in a training session at JSC's Weightless Environment Training Facility (WETF). Wearing the training version of the extravehicular mobility unit (EMU) space suit, weighted to allow neutral buoyancy in the 25 feet deep WETF pool, Krikalev minutes later was underwater simulating a contingency spacewalk, or extravehicular activity (EVA).

  14. Results and Analysis from Space Suit Joint Torque Testing

    Science.gov (United States)

    Matty, Jennifer

    2010-01-01

    This joint mobility KC lecture included information from two papers, "A Method for and Issues Associated with the Determination of Space Suit Joint Requirements" and "Results and Analysis from Space Suit Joint Torque Testing," as presented for the International Conference on Environmental Systems in 2009 and 2010, respectively. The first paper discusses historical joint torque testing methodologies and approaches that were tested in 2008 and 2009. The second paper discusses the testing that was completed in 2009 and 2010.

  15. STS-74 M.S. Jerry L. Ross suits up

    Science.gov (United States)

    1995-01-01

    Spaceflight veteran Jerry L. Ross, Mission Specialist 2 on Shuttle Mission STS-74, is assisted by a suit technician as he finishes getting into his launch/entry suit in the Operations and Checkout Building. Ross and four fellow astronauts will depart shortly for Launch Pad 39A, where the Space Shuttle Atlantis awaits a second liftoff attempt during a seven-minute window scheduled to open at approximately 7:30 a.m. EST, Nov. 12.

  16. Designing a suite of measurements to understand the critical zone

    Science.gov (United States)

    Brantley, Susan L.; DiBiase, Roman A.; Russo, Tess A.; Shi, Yuning; Lin, Henry; Davis, Kenneth J.; Kaye, Margot; Hill, Lillian; Kaye, Jason; Eissenstat, David M.; Hoagland, Beth; Dere, Ashlee L.; Neal, Andrew L.; Brubaker, Kristen M.; Arthur, Dan K.

    2016-03-01

    Many scientists have begun to refer to the earth surface environment from the upper canopy to the depths of bedrock as the critical zone (CZ). Identification of the CZ as an integral object worthy of study implicitly posits that the study of the whole earth surface will provide benefits that do not arise when studying the individual parts. To study the CZ, however, requires prioritizing among the measurements that can be made - and we do not generally agree on the priorities. Currently, the Susquehanna Shale Hills Critical Zone Observatory (SSHCZO) is expanding from a small original focus area (0.08 km2, Shale Hills catchment), to a larger watershed (164 km2, Shavers Creek watershed) and is grappling with the prioritization. This effort is an expansion from a monolithologic first-order forested catchment to a watershed that encompasses several lithologies (shale, sandstone, limestone) and land use types (forest, agriculture). The goal of the project remains the same: to understand water, energy, gas, solute, and sediment (WEGSS) fluxes that are occurring today in the context of the record of those fluxes over geologic time as recorded in soil profiles, the sedimentary record, and landscape morphology. Given the small size of the Shale Hills catchment, the original design incorporated measurement of as many parameters as possible at high temporal and spatial density. In the larger Shavers Creek watershed, however, we must focus the measurements. We describe a strategy of data collection and modeling based on a geomorphological and land use framework that builds on the hillslope as the basic unit. Interpolation and extrapolation beyond specific sites relies on geophysical surveying, remote sensing, geomorphic analysis, the study of natural integrators such as streams, groundwaters or air, and application of a suite of CZ models. We hypothesize that measurements of a few important variables at strategic locations within a geomorphological framework will allow

  17. COMPARISON OF 16S rRNA-PCR-RFLP, LipL32-PCR AND OmpL1-PCR METHODS IN THE DIAGNOSIS OF LEPTOSPIROSIS

    Directory of Open Access Journals (Sweden)

    Tülin GÜVEN GÖKMEN

    Full Text Available SUMMARY Leptospirosis is still one of the most important health problems in developing countries located in humid tropical and subtropical regions. Human infections are generally caused by exposure to water, soil or food contaminated with the urine of infected wild and domestic animals such as rodents and dogs. The clinical course of leptospirosis is variable and may be difficult to distinguish from many other infectious diseases. The dark-field microscopy (DFM, serology and nucleic acid amplification techniques are used to diagnose leptospirosis, however, a distinctive standard reference method is still lacking. Therefore, in this study, we aimed to determine the presence of Leptospira spp., to differentiate the pathogenic L. interrogans and the non-pathogenic L. biflexa, and also to determine the sensitivity and specificity values of molecular methods as an alternative to conventional ones. A total of 133 serum samples, from 47 humans and 86 cattle were evaluated by two conventional tests: the Microagglutination Test (MAT and the DFM, as well as three molecular methods, the 16S rRNA-PCR followed by Restriction Fragment Lenght Polymorphism (RFLP of the amplification products 16S rRNA-PCR-RFLP, LipL32-PCR and OmpL1-PCR. In this study, for L. interrogans, the specificity and sensitivity rates of the 16S rRNA-PCR and the LipL32-PCR were considered similar (100% versus 98.25% and 100% versus 98.68%, respectively. The OmpL1-PCR was able to classify L. interrogans into two intergroups, but this PCR was less sensitive (87.01% than the other two PCR methods. The 16S rRNA-PCR-RFLP could detect L. biflexa DNA, but LipL32-PCR and OmpL1-PCR could not. The 16S rRNA-PCR-RFLP provided an early and accurate diagnosis and was able to distinguish pathogenic and non-pathogenic Leptospira species, hence it may be used as an alternative method to the conventional gold standard techniques for the rapid disgnosis of leptospirosis.

  18. Immunoproteomic profiling of Rickettsia parkeri and Rickettsia amblyommii.

    Science.gov (United States)

    Pornwiroon, Walairat; Bourchookarn, Apichai; Paddock, Christopher D; Macaluso, Kevin R

    2015-09-01

    Rickettsia parkeri is an Amblyomma-associated, spotted fever group Rickettsia species that causes an eschar-associated, febrile illness in multiple countries throughout the Western Hemisphere. Many other rickettsial species of known or uncertain pathogenicity have been detected in Amblyomma spp. ticks in the Americas, including Rickettsia amblyommii, "Candidatus Rickettsia andeanae" and Rickettsia rickettsii. In this study, we utilized an immunoproteomic approach to compare antigenic profiles of low-passage isolates of R. parkeri and R. amblyommii with serum specimens from patients with PCR- and culture-confirmed infections with R. parkeri. Five immunoreactive proteins of R. amblyommii and nine immunoreactive proteins of R. parkeri were identified by matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry. Four of these, including the outer membrane protein (Omp) A, OmpB, translation initiation factor IF-2, and cell division protein FtsZ, were antigens common to both rickettsiae. Serum specimens from patients with R. parkeri rickettsiosis reacted specifically with cysteinyl-tRNA synthetase, DNA-directed RNA polymerase subunit alpha, putative sigma (54) modulation protein, chaperonin GroEL, and elongation factor Tu of R. parkeri which have been reported as virulence factors in other bacterial species. Unique antigens identified in this study may be useful for further development of the better serological assays for diagnosing infection caused by R. parkeri. Copyright © 2015 The Authors. Published by Elsevier GmbH.. All rights reserved.

  19. Determination of ocean/atmosphere carbon dioxide flux within OMP survey area. Final technical progress report, June, 1 1993--May 31, 1995

    Energy Technology Data Exchange (ETDEWEB)

    Chipman, D.W.; Takahashi, T.

    1995-10-17

    Determination of the net flux of atmospheric CO{sub 2} with the ocean at the continental margin is one of the three principal goals of the Ocean Margins Program. The work reported here represents the initial phase of that determination, as carried out during two cruises within the OMP survey area in 1993 and 1994. The interannual variability was addressed through the occupation of hydrographic stations of nearly identical location one year apart, while the spatial variability in the air-sea PCO{sub 2} difference (ApCO{sub 2}), representing the driving force for net CO{sub 2} flux, was addressed during a survey of much of the continental shelf between the survey area off North Carolina and Georges Bank. Not addressed by the initial cruises was the seasonal variability of the net CO{sub 2} flux, since both scoping cruises were mounted during the same season of the respective years.

  20. Amplification, cloning and expression of Brucella melitensis bp26 gene (OMP28 isolated from Markazi province (Iran and purification of Bp26 Protein

    Directory of Open Access Journals (Sweden)

    Hosseini, S.D.

    2013-12-01

    Full Text Available Brucellosis is a debilitative disease that imposes costs on both economy and society. It is shown that although the vaccine can prevent abortion, it does not provide complete protection against infection. In Iran, Brucella melitensis is a common causative agent for brucellosis and BP26 protein of this bacterium having a good antigenesity and an important vaccine candidate. In this study B. melitensis bp26 gene was cloned first in to PTZ57R/T vector and accessed on the PET28a vector and sequenced. Recombinant vector transformed and expressed in to E. coli BL21 (DE3 and then recombinant protein was purified with Ni-NTA column of chromatography against His tag. Obtained rOmp28 could be used as a research experimental tool to find its potential as a detection kit and vaccine candidate.

  1. Reliability performance testing of totally encapsulating chemical protective suits

    International Nuclear Information System (INIS)

    Johnson, J.S.; Swearengen, P.M.

    1991-01-01

    The need to assure a high degree of reliability for totally encapsulating chemical protective (TECP) suits has been recognized by Lawrence Livermore National Laboratory's (LLNL) Hazards Control Department for some time. The following four tests were proposed as necessary to provide complete evaluation of TECP suit performance: 1. Quantitative leak test (ASTM draft), 2. Worst-case chemical exposure test (conceptual), 3. Pressure leak-rate test (complete, ASTM F1057-87), and 4. Chemical leak-rate test (ASTM draft). This paper reports on these tests which should be applied to measuring TECP suit performance in two stages: design qualification tests and field use tests. Test 1, 2, and 3 are used as design qualification tests, and tests 3 and 4 are used as field use tests

  2. NetSuite OneWorld Implementation 2011 R2

    CERN Document Server

    Foydel, Thomas

    2011-01-01

    This book is a focused, step-by step tutorial that shows you how to successfully implement NetSuite OneWorld into your organization. It is written in an easy-to-read style, with a strong emphasis on real-world, practical examples with step-by-step explanations. The book focuses on NetSuite OneWorld 2011 R1. If you are an application administrator, business analyst, project team member or business process owner who wants to implement NetSuite OneWorld into your organization, then this book is for you. This book might also be useful if you are a business manager considering a new system for your

  3. Modulation of enrofloxacin binding in OmpF by Mg2+ as revealed by the analysis of fast flickering single-porin current

    Science.gov (United States)

    Brauser, Annemarie; Schroeder, Indra; Gutsmann, Thomas; Cosentino, Cristian; Moroni, Anna; Winterhalter, Mathias

    2012-01-01

    One major determinant of the efficacy of antibiotics on Gram-negative bacteria is the passage through the outer membrane. During transport of the fluoroquinolone enrofloxacin through the trimeric outer membrane protein OmpF of Escherichia coli, the antibiotic interacts with two binding sites within the pore, thus partially blocking the ionic current. The modulation of one affinity site by Mg2+ reveals further details of binding sites and binding kinetics. At positive membrane potentials, the slow blocking events induced by enrofloxacin in Mg2+-free media are converted to flickery sojourns at the highest apparent current level (all three pores flickering). This indicates weaker binding in the presence of Mg2+. Analysis of the resulting amplitude histograms with β distributions revealed the rate constants of blocking (kOB) and unblocking (kBO) in the range of 1,000 to 120,000 s−1. As expected for a bimolecular reaction, kOB was proportional to blocker concentration and kBO independent of it. kOB was approximately three times lower for enrofloxacin coming from the cis side than from the trans side. The block was not complete, leading to a residual conductivity of the blocked state being ∼25% of that of the open state. Interpretation of the results has led to the following model: fast flickering as caused by interaction of Mg2+ and enrofloxacin is related to the binding site at the trans side, whereas the cis site mediates slow blocking events which are also found without Mg2+. The difference in the accessibility of the binding sites also explains the dependency of kOB on the side of enrofloxacin addition and yields a means of determining the most plausible orientation of OmpF in the bilayer. The voltage dependence suggests that the dipole of the antibiotic has to be adequately oriented to facilitate binding. PMID:22689827

  4. Ultraviolet Testing of Space Suit Materials for Mars

    Science.gov (United States)

    Larson, Kristine; Fries, Marc

    2017-01-01

    Human missions to Mars may require radical changes in the approach to extra-vehicular (EVA) suit design. A major challenge is the balance of building a suit robust enough to complete multiple EVAs under intense ultraviolet (UV) light exposure without losing mechanical strength or compromising the suit's mobility. To study how the materials degrade on Mars in-situ, the Jet Propulsion Laboratory (JPL) invited the Advanced Space Suit team at NASA's Johnson Space Center (JSC) to place space suit materials on the Scanning Habitable Environments with Raman & Luminescence for Organics and Chemicals (SHERLOC) instrument's calibration target of the Mars 2020 rover. In order to select materials for the rover and understand the effects from Mars equivalent UV exposure, JSC conducted ground testing on both current and new space suit materials when exposed to 2500 hours of Mars mission equivalent UV. To complete this testing, JSC partnered with NASA's Marshall Space Flight Center to utilize their UV vacuum chambers. Materials tested were Orthofabric, polycarbonate, Teflon, Dacron, Vectran, spectra, bladder, nGimat coated Teflon, and nGimat coated Orthofabric. All samples were measured for mass, tensile strength, and chemical composition before and after radiation. Mass loss was insignificant (less than 0.5%) among the materials. Most materials loss tensile strength after radiation and became more brittle with a loss of elongation. Changes in chemical composition were seen in all radiated materials through Spectral Analysis. Results from this testing helped select the materials that will fly on the Mars 2020 rover. In addition, JSC can use this data to create a correlation to the chemical changes after radiation-which is what the rover will send back while on Mars-to the mechanical changes, such as tensile strength.

  5. STS-82 Pilot Scott J. 'Doc' Horowitz Suit Up

    Science.gov (United States)

    1997-01-01

    STS-82 Pilot Scott J. 'Doc' Horowitz puts on a glove of his launch and entry suit with assistance from a suit technician in the Operations and Checkout Building. This is Horowitz''';s second space flight. He and the six other crew members will depart shortly for Launch Pad 39A, where the Space Shuttle Discovery awaits liftoff on a 10-day mission to service the orbiting Hubble Space Telescope (HST). This will be the second HST servicing mission. Four back-to-back spacewalks are planned.

  6. STS-87 Mission Specialist Winston E. Scott suits up

    Science.gov (United States)

    1997-01-01

    STS-87 Mission Specialist Winston Scott dons his launch and entry suit with the assistance of a suit technician in the Operations and Checkout Building. This is Scotts second space flight. He and the five other crew members will depart shortly for Launch Pad 39B, where the Space Shuttle Columbia awaits liftoff on a 16-day mission to perform microgravity and solar research. Scott is scheduled to perform an extravehicular activity spacewalk with Mission Specialist Takao Doi, Ph.D., of the National Space Development Agency of Japan, during STS-87. He also performed a spacewalk on STS-72.

  7. Extending the GI Brokering Suite to Support New Interoperability Specifications

    Science.gov (United States)

    Boldrini, E.; Papeschi, F.; Santoro, M.; Nativi, S.

    2014-12-01

    The GI brokering suite provides the discovery, access, and semantic Brokers (i.e. GI-cat, GI-axe, GI-sem) that empower a Brokering framework for multi-disciplinary and multi-organizational interoperability. GI suite has been successfully deployed in the framework of several programmes and initiatives, such as European Union funded projects, NSF BCube, and the intergovernmental coordinated effort Global Earth Observation System of Systems (GEOSS). Each GI suite Broker facilitates interoperability for a particular functionality (i.e. discovery, access, semantic extension) among a set of brokered resources published by autonomous providers (e.g. data repositories, web services, semantic assets) and a set of heterogeneous consumers (e.g. client applications, portals, apps). A wide set of data models, encoding formats, and service protocols are already supported by the GI suite, such as the ones defined by international standardizing organizations like OGC and ISO (e.g. WxS, CSW, SWE, GML, netCDF) and by Community specifications (e.g. THREDDS, OpenSearch, OPeNDAP, ESRI APIs). Using GI suite, resources published by a particular Community or organization through their specific technology (e.g. OPeNDAP/netCDF) can be transparently discovered, accessed, and used by different Communities utilizing their preferred tools (e.g. a GIS visualizing WMS layers). Since Information Technology is a moving target, new standards and technologies continuously emerge and are adopted in the Earth Science context too. Therefore, GI Brokering suite was conceived to be flexible and accommodate new interoperability protocols and data models. For example, GI suite has recently added support to well-used specifications, introduced to implement Linked data, Semantic Web and precise community needs. Amongst the others, they included: DCAT: a RDF vocabulary designed to facilitate interoperability between Web data catalogs. CKAN: a data management system for data distribution, particularly used by

  8. Results from Carbon Dioxide Washout Testing Using a Suited Manikin Test Apparatus with a Space Suit Ventilation Test Loop

    Science.gov (United States)

    Chullen, Cinda; Conger, Bruce; McMillin, Summer; Vonau, Walt; Kanne, Bryan; Korona, Adam; Swickrath, Mike

    2016-01-01

    NASA is developing an advanced portable life support system (PLSS) to meet the needs of a new NASA advanced space suit. The PLSS is one of the most critical aspects of the space suit providing the necessary oxygen, ventilation, and thermal protection for an astronaut performing a spacewalk. The ventilation subsystem in the PLSS must provide sufficient carbon dioxide (CO2) removal and ensure that the CO2 is washed away from the oronasal region of the astronaut. CO2 washout is a term used to describe the mechanism by which CO2 levels are controlled within the helmet to limit the concentration of CO2 inhaled by the astronaut. Accumulation of CO2 in the helmet or throughout the ventilation loop could cause the suited astronaut to experience hypercapnia (excessive carbon dioxide in the blood). A suited manikin test apparatus (SMTA) integrated with a space suit ventilation test loop was designed, developed, and assembled at NASA in order to experimentally validate adequate CO2 removal throughout the PLSS ventilation subsystem and to quantify CO2 washout performance under various conditions. The test results from this integrated system will be used to validate analytical models and augment human testing. This paper presents the system integration of the PLSS ventilation test loop with the SMTA including the newly developed regenerative Rapid Cycle Amine component used for CO2 removal and tidal breathing capability to emulate the human. The testing and analytical results of the integrated system are presented along with future work.

  9. PLEASE: The Python Low-energy Electron Analysis SuitE – Enabling Rapid Analysis of LEEM and LEED Data

    Directory of Open Access Journals (Sweden)

    Maxwell Grady

    2018-02-01

    Full Text Available PLEASE, the Python Low-energy Electron Analysis SuitE, provides an open source and cross-platform graphical user interface (GUI for rapid analysis and visualization of low energy electron microscopy (LEEM data sets. LEEM and the associated technique, selected area micro-spot low energy electron diffraction (μ-LEED, are powerful tools for analysis of the surface structure for many novel materials. Specifically, these tools are uniquely suited for the characterization of two-dimensional materials. PLEASE offers a user-friendly point-and-click method for extracting intensity-voltage curves from LEEM and LEED data sets. Analysis of these curves provides insight into the atomic structure of the target material surface with unparalleled resolution.

  10. The Los Alamos suite of relativistic atomic physics codes

    International Nuclear Information System (INIS)

    Fontes, C J; Zhang, H L; Jr, J Abdallah; Clark, R E H; Kilcrease, D P; Colgan, J; Cunningham, R T; Hakel, P; Magee, N H; Sherrill, M E

    2015-01-01

    The Los Alamos suite of relativistic atomic physics codes is a robust, mature platform that has been used to model highly charged ions in a variety of ways. The suite includes capabilities for calculating data related to fundamental atomic structure, as well as the processes of photoexcitation, electron-impact excitation and ionization, photoionization and autoionization within a consistent framework. These data can be of a basic nature, such as cross sections and collision strengths, which are useful in making predictions that can be compared with experiments to test fundamental theories of highly charged ions, such as quantum electrodynamics. The suite can also be used to generate detailed models of energy levels and rate coefficients, and to apply them in the collisional-radiative modeling of plasmas over a wide range of conditions. Such modeling is useful, for example, in the interpretation of spectra generated by a variety of plasmas. In this work, we provide a brief overview of the capabilities within the Los Alamos relativistic suite along with some examples of its application to the modeling of highly charged ions. (paper)

  11. Measuring Test Case Similarity to Support Test Suite Understanding

    NARCIS (Netherlands)

    Greiler, M.S.; Van Deursen, A.; Zaidman, A.E.

    2012-01-01

    Preprint of paper published in: TOOLS 2012 - Proceedings of the 50th International Conference, Prague, Czech Republic, May 29-31, 2012; doi:10.1007/978-3-642-30561-0_8 In order to support test suite understanding, we investigate whether we can automatically derive relations between test cases. In

  12. Knowledge Architect : A Tool Suite for Managing Software Architecture Knowledge

    NARCIS (Netherlands)

    Liang, Peng; Jansen, Anton; Avgeriou, Paris

    2009-01-01

    Management of software architecture knowledge (AK) is vital for improving an organization’s architectural capabilities. To support the architecting process within our industrial partner: Astron, the Dutch radio astronomy institute, we implemented the Knowledge Architect (KA): a tool suite for

  13. The Zoot Suit Riots: Exploring Social Issues in American History

    Science.gov (United States)

    Chiodo, John J.

    2013-01-01

    The Zoot Suit Riots provide students with a case study of social unrest in American history. The influx of Latinos into the Los Angeles area prior to World War II created high levels of social unrest between Mexican Americans, military servicemen, and local residences. With large numbers of soldiers stationed in the area during the Second World…

  14. 28 CFR 36.503 - Suit by the Attorney General.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Suit by the Attorney General. 36.503... discretion, the Attorney General may commence a civil action in any appropriate United States district court if the Attorney General has reasonable cause to believe that— (a) Any person or group of persons is...

  15. DYNA3D/ParaDyn Regression Test Suite Inventory

    Energy Technology Data Exchange (ETDEWEB)

    Lin, Jerry I. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2016-09-01

    The following table constitutes an initial assessment of feature coverage across the regression test suite used for DYNA3D and ParaDyn. It documents the regression test suite at the time of preliminary release 16.1 in September 2016. The columns of the table represent groupings of functionalities, e.g., material models. Each problem in the test suite is represented by a row in the table. All features exercised by the problem are denoted by a check mark (√) in the corresponding column. The definition of “feature” has not been subdivided to its smallest unit of user input, e.g., algorithmic parameters specific to a particular type of contact surface. This represents a judgment to provide code developers and users a reasonable impression of feature coverage without expanding the width of the table by several multiples. All regression testing is run in parallel, typically with eight processors, except problems involving features only available in serial mode. Many are strictly regression tests acting as a check that the codes continue to produce adequately repeatable results as development unfolds; compilers change and platforms are replaced. A subset of the tests represents true verification problems that have been checked against analytical or other benchmark solutions. Users are welcomed to submit documented problems for inclusion in the test suite, especially if they are heavily exercising, and dependent upon, features that are currently underrepresented.

  16. Safety Tips: Avoiding Negligence Suits in Chemistry Teaching.

    Science.gov (United States)

    Gerlovich, Jack A.

    1983-01-01

    Discusses various aspects related to negligence on the part of chemistry teachers. Areas addressed include negligence in tort law, avoiding negligence suits, proper instructions, proper supervision, equipment maintenance, and other considerations such as sovereign immunity, and contributory versus comparative negligence. (JN)

  17. Automated integration of lidar into the LANDFIRE product suite

    Science.gov (United States)

    Birgit Peterson; Kurtis J. Nelson; Carl Seielstad; Jason Stoker; W. Matt Jolly; Russell Parsons

    2015-01-01

    Accurate information about three-dimensional canopy structure and wildland fuel across the landscape is necessary for fire behaviour modelling system predictions. Remotely sensed data are invaluable for assessing these canopy characteristics over large areas; lidar data, in particular, are uniquely suited for quantifying three-dimensional canopy structure. Although...

  18. ASIM - an Instrument Suite for the International Space Station

    DEFF Research Database (Denmark)

    Neubert, Torsten; Crosby, B.; Huang, T.-Y.

    2009-01-01

    ASIM (Atmosphere-Space Interactions Monitor) is an instrument suite for studies of severe thunderstorms and their effects on the atmosphere and ionosphere. The instruments are designed to observe transient luminous events (TLEs)—sprites, blue jets and elves—and terrestrial gamma-ray flashes (TGFs...

  19. Rimsky-Korsakov: Symphony N2 (Symphonic Suite) / Warrack, John

    Index Scriptorium Estoniae

    Warrack, John

    1990-01-01

    Uuest heliplaadist "Rimsky-Korsakov: Symphony N2 (Symphonic Suite), Op. 9, "Antar" Russian Easter Festival Overture, Op.36. Philharmonia Orchestra, Evgeni Svetlanov. Hyperion KA 66399. CDA 66399. Teise sümfoonia esitust võrreldud Neeme Järvi plaadistusega

  20. Heat and mass transfer in air-fed pressurised suits

    International Nuclear Information System (INIS)

    Tesch, K.; Collins, M.W.; Karayiannis, T.G.; Atherton, M.A.; Edwards, P.

    2009-01-01

    Air-fed pressurised suits are used to protect workers against contamination and hazardous environments. The specific application here is the necessity for regular clean-up maintenance within the torus chamber of fusion reactors. The current design of suiting has been developed empirically. It is, therefore, very desirable to formulate a thermo-fluids model, which will be able to define optimum designs and operating parameters. Two factors indicate that the modelling should be as comprehensive as possible. Firstly, the overall thermo-fluids problem is three-dimensional and includes mass as well as heat transfer. The fluid field is complex, bounded on one side by the human body and on the other by what may be distensible, porous and multi-layer clothing. In this paper, we report firstly the modelling necessary for the additional mass and heat transport processes. This involves the use of Fick's and Fourier's laws and conjugate heat transfer. The results of an initial validation study are presented. Temperatures at the outlet of the suits were obtained experimentally and compared with those predicted by the overall CFD model. Realistic three-dimensional geometries were used for the suit and human body. Calculations were for turbulent flow with single- and two-component (species) models

  1. Tailoring Vantage 5 (fuel) to suit each operator's need

    Energy Technology Data Exchange (ETDEWEB)

    Chapin, D L; Secker, J R [Westinghouse Electric Corp., Philadelphia, PA (USA)

    1990-03-01

    By the end of 1989, Westinghouse Vantage 5 fuel had been reloaded into 36 nuclear power plants. The fuel offers a number of features operators can choose from to suit their own particular needs. Experience so far has shown the fuel to have performed well, with coolant activity levels remaining low. (author).

  2. Exploration Space Suit Architecture: Destination Environmental-Based Technology Development

    Science.gov (United States)

    Hill, Terry R.

    2010-01-01

    This paper picks up where EVA Space Suit Architecture: Low Earth Orbit Vs. Moon Vs. Mars (Hill, Johnson, IEEEAC paper #1209) left off in the development of a space suit architecture that is modular in design and interfaces and could be reconfigured to meet the mission or during any given mission depending on the tasks or destination. This paper will walk though the continued development of a space suit system architecture, and how it should evolve to meeting the future exploration EVA needs of the United States space program. In looking forward to future US space exploration and determining how the work performed to date in the CxP and how this would map to a future space suit architecture with maximum re-use of technology and functionality, a series of thought exercises and analysis have provided a strong indication that the CxP space suit architecture is well postured to provide a viable solution for future exploration missions. Through the destination environmental analysis that is presented in this paper, the modular architecture approach provides the lowest mass, lowest mission cost for the protection of the crew given any human mission outside of low Earth orbit. Some of the studies presented here provide a look and validation of the non-environmental design drivers that will become every-increasingly important the further away from Earth humans venture and the longer they are away. Additionally, the analysis demonstrates a logical clustering of design environments that allows a very focused approach to technology prioritization, development and design that will maximize the return on investment independent of any particular program and provide architecture and design solutions for space suit systems in time or ahead of being required for any particular manned flight program in the future. The new approach to space suit design and interface definition the discussion will show how the architecture is very adaptable to programmatic and funding changes with

  3. Observing atmospheric formaldehyde (HCHO from space: validation and intercomparison of six retrievals from four satellites (OMI, GOME2A, GOME2B, OMPS with SEAC4RS aircraft observations over the southeast US

    Directory of Open Access Journals (Sweden)

    L. Zhu

    2016-11-01

    Full Text Available Formaldehyde (HCHO column data from satellites are widely used as a proxy for emissions of volatile organic compounds (VOCs, but validation of the data has been extremely limited. Here we use highly accurate HCHO aircraft observations from the NASA SEAC4RS (Studies of Emissions, Atmospheric Composition, Clouds and Climate Coupling by Regional Surveys campaign over the southeast US in August–September 2013 to validate and intercompare six retrievals of HCHO columns from four different satellite instruments (OMI, GOME2A, GOME2B and OMPS; for clarification of these and other abbreviations used in the paper, please refer to Appendix A and three different research groups. The GEOS-Chem chemical transport model is used as a common intercomparison platform. All retrievals feature a HCHO maximum over Arkansas and Louisiana, consistent with the aircraft observations and reflecting high emissions of biogenic isoprene. The retrievals are also interconsistent in their spatial variability over the southeast US (r  =  0.4–0.8 on a 0.5°  ×  0.5°  grid and in their day-to-day variability (r  =  0.5–0.8. However, all retrievals are biased low in the mean by 20–51 %, which would lead to corresponding bias in estimates of isoprene emissions from the satellite data. The smallest bias is for OMI-BIRA, which has high corrected slant columns relative to the other retrievals and low scattering weights in its air mass factor (AMF calculation. OMI-BIRA has systematic error in its assumed vertical HCHO shape profiles for the AMF calculation, and correcting this would eliminate its bias relative to the SEAC4RS data. Our results support the use of satellite HCHO data as a quantitative proxy for isoprene emission after correction of the low mean bias. There is no evident pattern in the bias, suggesting that a uniform correction factor may be applied to the data until better understanding is achieved.

  4. Characterization and immunogenicity of rLipL32/1-LipL21-OmpL1/2 fusion protein as a novel immunogen for a vaccine against Leptospirosis

    Directory of Open Access Journals (Sweden)

    Zhao Xin

    2015-01-01

    Full Text Available Vaccination is an effective strategy to prevent leptospirosis, a global zoonotic disease caused by infection with pathogenic Leptospira species. However, the currently used multiple-valence vaccine, which is prepared with whole cells of several Leptospira serovars, has major side effects, while its cross-immunogenicity among different Leptospira serovars is weak. LipL32, LipL21 and 2 OmpL1 have been confirmed as surface-exposed antigens in all pathogenic Leptospira strains, but their immunoprotective efficiency needs to be improved. In the present study, we generated a fusion gene lipL32/1-lipL21-ompL1/2 using primer-linking PCR and an engineered E. coli strain to express the recombinant fusion protein rLipL32/1-LipL21-OmpL1/2 (rLLO. Subsequently, the expression conditions were optimized using a central composite design that increased the fusion protein yield 2.7-fold. Western blot assays confirmed that rLLO was recognized by anti-rLipL32/1, anti-rLipL21, and anti-rOmpL1/2 sera as well as 98.5% of the sera from leptospirosis patients. The microscopic agglutination test (MAT demonstrated that rLLO antiserum had a stronger ability to agglutinate the strains of different Leptospira serovars than the rLipL32/1, rLipL21, and rOmpL1/2 antisera. More importantly, tests in hamsters showed that rLLO provided higher immunoprotective rates (91.7% than rLipL32/1, rLipL21 and rOmpL1/2 (50.0-75.0%. All the data indicate that rLLO, a recombinant fusion protein incorporating three antigens, has increased antigenicity and immunoprotective effects, and so can be used as a novel immunogen to develop a universal genetically engineered vaccine against leptospirosis.

  5. Prokofieff: Krieg und Frieden (Sinfonische Suite), Die Verlobung im Kloster (Sommernacht-Suite), Russische Overtüre. Philharmonia Orchestra, Neeme Järvi / G. W.

    Index Scriptorium Estoniae

    G. W.

    1993-01-01

    Uuest heliplaadist "Prokofieff: Krieg und Frieden (Sinfonische Suite), Die Verlobung im Kloster (Sommernacht-Suite), Russische Overtüre. Philharmonia Orchestra, Neeme Järvi. (AD: 1991). Chandos/Koch CD 9096

  6. STS-95 Mission Specialist Pedro Duque suits up for launch

    Science.gov (United States)

    1998-01-01

    STS-95 Mission Specialist Pedro Duque of Spain, with the European Space Agency, is helped with his flight suit by suit tech Tommy McDonald in the Operations and Checkout Building. The final fitting takes place prior to the crew walkout and transport to Launch Pad 39B. Targeted for launch at 2 p.m. EST on Oct. 29, the mission is expected to last 8 days, 21 hours and 49 minutes, and return to KSC at 11:49 a.m. EST on Nov. 7. The STS-95 mission includes research payloads such as the Spartan solar-observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as the SPACEHAB single module with experiments on space flight and the aging process.

  7. Failure to exercise due diligence costs plaintiff her suit.

    Science.gov (United States)

    1997-11-28

    The Mississippi State Supreme Court affirmed a lower court ruling dismissing a last-minute suit filed by a plaintiff against United Blood Services of Mississippi and the American Association of Blood Banks. A woman known as D. Doe was a recipient of a tainted transfusion. She contracted HIV in 1983 and died of AIDS-related causes in 1991. Her daughter, the plaintiff, filed a contaminated blood transfusion lawsuit just five days before the statute of limitations ran out but failed to ascertain the correct identity of the blood bank. She named two blood banks in her suit because she was unable to determine the source of the blood. The Supreme Court ruled that waiting until five days before the statute elapsed indicated that the plaintiff did not exercise reasonable diligence within a specific time frame.

  8. STS-90 Pilot Scott Altman is suited up for launch

    Science.gov (United States)

    1998-01-01

    STS-90 Pilot Scott Altman is assisted during suit-up activities by Lockheed Suit Technician Valerie McNeil from Johnson Space Center in KSC's Operations and Checkout Building. Altman and the rest of the STS-90 crew will shortly depart for Launch Pad 39B, where the Space Shuttle Columbia awaits a second liftoff attempt at 2:19 p.m. EDT. His first trip into space, Altman is participating in a life sciences research flight that will focus on the most complex and least understood part of the human body - - the nervous system. Neurolab will examine the effects of spaceflight on the brain, spinal cord, peripheral nerves and sensory organs in the human body.

  9. Mission Specialist Scott Parazynski checks his flight suit

    Science.gov (United States)

    1998-01-01

    STS-95 Mission Specialist Scott E. Parazynski gets help with his flight suit in the Operations and Checkout Building from a suit technician George Brittingham. The final fitting takes place prior to the crew walkout and transport to Launch Pad 39B. Targeted for launch at 2 p.m. EST on Oct. 29, the mission is expected to last 8 days, 21 hours and 49 minutes, and return to KSC at 11:49 a.m. EST on Nov. 7. The STS-95 mission includes research payloads such as the Spartan solar-observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as the SPACEHAB single module with experiments on space flight and the aging process.

  10. STS-76 Payload Cmdr Ronald Sega suits up

    Science.gov (United States)

    1996-01-01

    STS-76 Payload Commander Ronald M. Sega is donning his launch/entry suit in the Operations and Checkout Building with assistance from a suit technician. The third docking between the Russian Space Station Mir and the U.S. Space Shuttle marks the second trip into space for Sega, who recently served a five-month assignment in Russia as operations director for NASA activities there. Once suitup activities are completed the six-member STS-76 flight crew will depart for Launch Pad 39B, where the Space Shuttle Atlantis is undergoing final preparations for liftoff during an approximately seven-minute launch window opening around 3:13 a.m. EST, March 22.

  11. The IMBA suite: integrated modules for bioassay analysis

    Energy Technology Data Exchange (ETDEWEB)

    Birchall, A.; Jarvis, N.S.; Peace, M.S.; Riddell, A.E.; Battersby, W.P

    1998-07-01

    The increasing complexity of models representing the biokinetic behaviour of radionuclides in the body following intake poses problems for people who are required to implement these models. The problem is exacerbated by the current paucity of suitable software. In order to remedy this situation, a collaboration between British Nuclear Fuels, Westlakes Research Institute and the National Radiological Protection Board has started with the aim of producing a suite of modules for estimating intakes and doses from bioassay measurements using the new ICRP models. Each module will have a single purpose (e.g. to calculate respiratory tract deposition) and will interface with other software using data files. The elements to be implemented initially are plutonium, uranium, caesium, iodine and tritium. It is intended to make the software available to other parties under terms yet to be decided. This paper describes the proposed suite of integrated modules for bioassay analysis, IMBA. (author)

  12. Enhanced verification test suite for physics simulation codes

    Energy Technology Data Exchange (ETDEWEB)

    Kamm, James R.; Brock, Jerry S.; Brandon, Scott T.; Cotrell, David L.; Johnson, Bryan; Knupp, Patrick; Rider, William J.; Trucano, Timothy G.; Weirs, V. Gregory

    2008-09-01

    This document discusses problems with which to augment, in quantity and in quality, the existing tri-laboratory suite of verification problems used by Los Alamos National Laboratory (LANL), Lawrence Livermore National Laboratory (LLNL), and Sandia National Laboratories (SNL). The purpose of verification analysis is demonstrate whether the numerical results of the discretization algorithms in physics and engineering simulation codes provide correct solutions of the corresponding continuum equations.

  13. Non-Venting Thermal and Humidity Control for EVA Suits

    Science.gov (United States)

    Izenson, Mike; Chen, Weibo; Bue, Grant

    2011-01-01

    Future EVA suits need processes and systems to control internal temperature and humidity without venting water to the environment. This paper describes an absorption-based cooling and dehumidification system as well as laboratory demonstrations of the key processes. There are two main components in the system: an evaporation cooling and dehumidification garment (ECDG) that removes both sensible heat and latent heat from the pressure garment, and an absorber radiator that absorbs moisture and rejects heat to space by thermal radiation. This paper discusses the overall design of both components, and presents recent data demonstrating their operation. We developed a design and fabrication approach to produce prototypical heat/water absorbing elements for the ECDG, and demonstrated by test that these elements could absorb heat and moisture at a high flux. Proof-of-concept tests showed that an ECDG prototype absorbs heat and moisture at a rate of 85 W/ft under conditions that simulate operation in an EVA suit. The heat absorption was primarily due to direct absorption of water vapor. It is possible to construct large, flexible, durable cooling patches that can be incorporated into a cooling garment with this system. The proof-of-concept test data was scaled to calculate area needed for full metabolic loads, thus showing that it is feasible to use this technology in an EVA suit. Full-scale, lightweight absorber/radiator modules have also been built and tested. They can reject heat at a flux of 33 W/ft while maintaining ECDG operation at conditions that will provide a cool and dry environment inside the EVA suit.

  14. Compression under a mechanical counter pressure space suit glove

    Science.gov (United States)

    Waldie, James M A.; Tanaka, Kunihiko; Tourbier, Dietmar; Webb, Paul; Jarvis, Christine W.; Hargens, Alan R.

    2002-01-01

    Background: Current gas-pressurized space suits are bulky stiff shells severely limiting astronaut function and capability. A mechanical counter pressure (MCP) space suit in the form of a tight elastic garment could dramatically improve extravehicular activity (EVA) dexterity, but also be advantageous in safety, cost, mass and volume. The purpose of this study was to verify that a prototype MCP glove exerts the design compression of 200 mmHg, a pressure similar to the current NASA EVA suit. Methods: Seven male subjects donned a pressure measurement array and MCP glove on the right hand, which was placed into a partial vacuum chamber. Average compression was recorded on the palm, the bottom of the middle finger, the top of the middle finger and the dorsum of the hand at pressures of 760 (ambient), 660 and 580 mmHg. The vacuum chamber was used to simulate the pressure difference between the low breathing pressure of the current NASA space suits (approximately 200 mmHg) and an unprotected hand in space. Results: At ambient conditions, the MCP glove compressed the dorsum of the hand at 203.5 +/- 22.7 mmHg, the bottom of the middle finger at 179.4 +/- 16.0 mmHg, and the top of the middle finger at 183.8 +/- 22.6 mmHg. The palm compression was significantly lower (59.6 +/- 18.8 mmHg, pglove compression with the chamber pressure reductions. Conclusions: The MCP glove compressed the dorsum of the hand and middle finger at the design pressure.

  15. Astronaut Ronald Evans is suited up for EVA training

    Science.gov (United States)

    1972-01-01

    Astronaut Ronald E. Evans, command module pilot of the Apollo 17 lunar landing mission, is assisted by technicians in suiting up for extravehicular activity (EVA) training in a water tank in bldg 5 at the Manned Spacecraft Center (49970); Evans participates in EVA training in a water tank in bldg 5 at the Manned Spacecraft Center. The structure in the picture simulates the Scientific Instrument Module (SIM) bay of the Apollo 17 Service Module (49971).

  16. Apollo 11 astronaut Neil Armstrong suits up before launch

    Science.gov (United States)

    1969-01-01

    Apollo 11 Commander Neil Armstrong prepares to put on his helmet with the assistance of a spacesuit technician during suiting operations in the Manned Spacecraft Operations Building (MSOB) prior to the astronauts' departure to Launch Pad 39A. The three astronauts, Edwin E. Aldrin Jr., Neil A Armstrong and Michael Collins, will then board the Saturn V launch vehicle, scheduled for a 9:32 a.m. EDT liftoff, for the first manned lunar landing mission.

  17. The Sample Analysis at Mars Investigation and Instrument Suite

    Science.gov (United States)

    Mahaffy, Paul; Webster, Christopher R.; Conrad, Pamela G.; Arvey, Robert; Bleacher, Lora; Brinckerhoff, William B.; Eigenbrode, Jennifer L.; Chalmers, Robert A.; Dworkin, Jason P.; Errigo, Therese; hide

    2012-01-01

    The Sample Analysis at Mars (SAM) investigation of the Mars Science Laboratory (MSL) addresses the chemical and isotopic composition of the atmosphere and volatiles extracted from solid samples. The SAM investigation is designed to contribute substantially to the mission goal of quantitatively assessing the habitability of Mars as an essential step in the search for past or present life on Mars. SAM is a 40 kg instrument suite located in the interior of MSL's Curiosity rover. The SAM instruments are a quadrupole mass spectrometer, a tunable laser spectrometer, and a 6-column gas chromatograph all coupled through solid and gas processing systems to provide complementary information on the same samples. The SAM suite is able to measure a suite of light isotopes and to analyze volatiles directly from the atmosphere or thermally released from solid samples. In addition to measurements of simple inorganic compounds and noble gases SAM will conduct a sensitive search for organic compounds with either thermal or chemical extraction from sieved samples delivered by the sample processing system on the Curiosity rover's robotic arm,

  18. Statutes of limitations: the special problem of DES suits

    International Nuclear Information System (INIS)

    Feigin, C.A.

    1981-01-01

    In 1971, medical studies determined that DES causes a rare type of vaginal cancer in a small number of daughters of mothers who took DES during pregnancy. Subsequently, medical studies determined that exposure to DES can cause other vaginal abnormalities in the daughters, some of which may be precancerous. As a result of these discoveries, many lawsuits have been filed by these daughters against DES manufacturers. Many DES suits may be barred by statutes of limitations, both because the number of years between the daughters' exposure to DES in utero and the discovery that DES can cause injuries exceeds the statutory period, and because the cancer or other injuries caused by DES may not develop for many additional years. This Note discusses two methods that DES plaintiffs may be able to use to overcome the potential statutes of limitations bar: the discovery rule, and state provisions which toll the statute of limitations for minors. The Note contends that courts should apply an expanded discovery rule to DES suits to avoid the unfair result of barring a claim before the plaintiff could have known that she had a cause of action. In addition, the Note argues that the injury which causes the statute of limitations to begin to run in DES suits should not be rigidly defined. Finally, the Note urges that courts allow eligible DES plaintiffs to take advantage of applicable state provisions that toll the statute of limitations for minors

  19. 46 CFR 199.214 - Immersion suits and thermal protective aids.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Immersion suits and thermal protective aids. 199.214... Passenger Vessels § 199.214 Immersion suits and thermal protective aids. (a) Each passenger vessel must... an immersion suit. (c) The immersion suits and thermal protective aids required under paragraphs (a...

  20. 33 CFR 150.518 - What are the inspection requirements for work vests and immersion suits?

    Science.gov (United States)

    2010-07-01

    ... requirements for work vests and immersion suits? 150.518 Section 150.518 Navigation and Navigable Waters COAST... vests and immersion suits? (a) All work vests and immersion suits must be inspected by the owner or... a work vest or immersion suit is inspected and is in serviceable condition, then it may remain in...

  1. Comprehensive understandings of energy confinement in LHD plasmas through extensive application of the integrated transport analysis suite

    International Nuclear Information System (INIS)

    Yokoyama, M.; Seki, R.; Suzuki, C.; Ida, K.; Osakabe, M.; Satake, S.; Yamada, H.; Murakami, S.

    2014-10-01

    The integrated transport analysis suite, TASK3D-a, has enhanced energy transport analyses in LHD. It has clearly elucidated (1) the systematic dependence of ion and electron energy confinement on wide variation of plasma parameters, and (2) statistically-derived fitting expressions for the ion and electron heat diffusivities (χ i and χ e ), separately, taking also those radial-profile information into account. In particular, the latter approach can outstrip the conventional scaling laws for the global confinement time (τ E ) in terms of its considerations on profiles (temperature, density, heating depositions etc.). This has been made possible with the analysis database accumulated by the extensive application of the integrated transport analysis suite to experiment data. In this proceeding, TASK3D-a analysis-database for high-ion-temperature (high-T i ) plasmas in LHD (Large Helical Device) are exemplified. This approach should be applicable to any other combinations of integrated transport analysis suites and fusion experiments. (author)

  2. A deterministic electron, photon, proton and heavy ion transport suite for the study of the Jovian moon Europa

    International Nuclear Information System (INIS)

    Badavi, Francis F.; Blattnig, Steve R.; Atwell, William; Nealy, John E.; Norman, Ryan B.

    2011-01-01

    extended mission. For the upcoming EJSM mission with an expected launch date of 2020, the transport suite is used to compute the depth dose profile for the traditional aluminum silicon as a standard shield target combination, as well as simulating the shielding response of a high charge number (Z) material such as tantalum (Ta). Finally, a shield optimization algorithm is discussed which can guide the instrument designers and fabrication personnel with the choice of graded-Z shield selection and analysis.

  3. Virulence gene profiles of avian pathogenic Escherichia coli isolated from chickens with colibacillosis in Bulawayo, Zimbabwe

    Directory of Open Access Journals (Sweden)

    Joshua Mbanga

    2015-04-01

    Full Text Available Colibacillosis, a disease caused by avian pathogenic Escherichia coli (APEC, is one of the main causes of economic losses in the poultry industry worldwide. This study was carried out in order to determine the APEC-associated virulence genes contained by E. coli isolates causing colibacillosis in chickens. A total of 45 E. coli isolates were obtained from the diagnostics and research branch of the Central Veterinary Laboratories, Bulawayo, Zimbabwe. These isolates were obtained from chickens with confirmed cases of colibacillosis after postmortem examination. The presence of the iutA, hlyF, ompT, frz, sitD, fimH, kpsM, sitA, sopB, uvrY, pstB and vat genes were investigated by multiplex polymerase chain reaction (PCR assay. Of the 45 isolates, 93% were positive for the presence of at least one virulence gene. The three most prevalent virulence genes were iutA (80%, fimH (33.3% and hlyF (24.4%. The kpsM, pstB and ompT genes had the lowest prevalence, having been detected in only 2.2% of the isolates. All 12 virulence genes studied were detected in the 45 APEC isolates. Virulence gene profiles were constructed for each APEC isolate from the multiplex data. The APEC isolates were profiled as 62.2% fitting profile A, 31.1% profile B and 6.7% profile C. None of the isolates had more than seven virulence genes. Virulence profiles of Zimbabwean APEC isolates are different from those previously reported. Zimbabwean APEC isolates appear to be less pathogenic and may rely on environmental factors and stress in hosts to establish infection.

  4. Inducción de IgA e IgG anti-omp48 en conejos inmunizados intragástricamente con una fracción extracelular de Aeromonas veronii

    Directory of Open Access Journals (Sweden)

    Eduardo Ruiz-Bustos

    2011-01-01

    Full Text Available Una proteína de membrana externa de 48 kDa (omp48 de Aeromonas veronii (A. veronii, que tiene afinidad por secreciones de la mucosa (tal como mucina, lactoferrina, inmunoglobulinas o colágeno, induce una respuesta inmune estadísticamente significativa al ser administrada intragástricamente a conejos blancos de Nueva Zelanda. Estudios posteriores mostraron una correlación positiva entre las inmunoglobulinas y la respuesta de células productoras de anticuerpo (CPA. La administración intragástrica del antígeno induce una respuesta vigorosa, tanto local como sistémica, principalmente de inmunoglobulinas A y G contra la omp48 de A. veronii. Estas se evidenciaron por la presencia de (i células productoras de anticuerpos contra omp48 en el tracto gastrointestinal, bazo y sangre, y (ii elevados niveles de anticuerpos circulantes.La estimulación del sistema inmune de mucosa mediante antígenos bacterianos inmunogénicos, puede ser una importante alternativa para el control y prevención de enfermedades asociadas a Aeromonas.

  5. Suited versus unsuited analog astronaut performance using the Aouda.X space suit simulator: the DELTA experiment of MARS2013.

    Science.gov (United States)

    Soucek, Alexander; Ostkamp, Lutz; Paternesi, Roberta

    2015-04-01

    Space suit simulators are used for extravehicular activities (EVAs) during Mars analog missions. Flight planning and EVA productivity require accurate time estimates of activities to be performed with such simulators, such as experiment execution or traverse walking. We present a benchmarking methodology for the Aouda.X space suit simulator of the Austrian Space Forum. By measuring and comparing the times needed to perform a set of 10 test activities with and without Aouda.X, an average time delay was derived in the form of a multiplicative factor. This statistical value (a second-over-second time ratio) is 1.30 and shows that operations in Aouda.X take on average a third longer than the same operations without the suit. We also show that activities predominantly requiring fine motor skills are associated with larger time delays (between 1.17 and 1.59) than those requiring short-distance locomotion or short-term muscle strain (between 1.10 and 1.16). The results of the DELTA experiment performed during the MARS2013 field mission increase analog mission planning reliability and thus EVA efficiency and productivity when using Aouda.X.

  6. FRHAM-TEX trademark cool suit - OST reference No. 1854. Deactivation and decommissioning focus area

    International Nuclear Information System (INIS)

    1998-02-01

    This paper describes a demonstration project for the FRHAM-TEX Cool Suit trademark manufactured by FRHAM Safety Products. It is a one-piece, disposable, breathable, waterproof coverall designed to permit moisture generated by the wearer to be transmitted outside the suit. The performance of this suit was compared to a Tyvek reg-sign suit as a baseline. The suit is proposed as safety ware for workers at decontamination and decommissioning projects

  7. Data Profiling

    OpenAIRE

    Hladíková, Radka

    2010-01-01

    Title: Data Profiling Author: Radka Hladíková Department: Department of Software Engineering Supervisor: Ing. Vladimír Kyjonka Supervisor's e-mail address: Abstract: This thesis puts mind on problems with data quality and data profiling. This Work analyses and summarizes problems of data quality, data defects, process of data quality, data quality assessment and data profiling. The main topic is data profiling as a process of researching data available in existing...

  8. Novel vector vaccine against Brucella abortus based on influenza A viruses expressing Brucella L7/L12 or Omp16 proteins: evaluation of protection in pregnant heifers.

    Science.gov (United States)

    Tabynov, Kaissar; Yespembetov, Bolat; Sansyzbay, Abylai

    2014-10-14

    The present study provides the first information about the protection of a novel influenza viral vector vaccine expressing the Brucella proteins ribosomal L7/L12 or Omp16 containing the adjuvant Montanide Gel01 in pregnant heifers. Immunization of pregnant heifers was conducted via the conjunctival (n=10) or subcutaneous (n=10) route using cross prime and booster vaccination schedules at an interval of 28 days. The vector vaccine was evaluated in comparison with positive control groups vaccinated with Brucella abortus S19 (n=10) or B. abortus RB51 (n=10) and a negative (PBS+Montanide Gel01; n=10) control group. Via both the conjunctival or subcutaneous route, evaluation of protectiveness against abortion, effectiveness of vaccination and index of infection (in heifers and their fetuses or calves) demonstrated the vector vaccine provided good protection against B. abortus 544 infection compared to the negative control group (PBS+Montanide Gel01) and comparable protection to commercial vaccines B. abortus S19 or B. abortus RB51. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Structure analysis of OmpC, one of the major proteins in the outer membrane of E. coli, by high resolution electron microscopy

    International Nuclear Information System (INIS)

    Chang, C.F.

    1983-07-01

    This dissertation is concerned with the structure analysis of a pore-forming membrane protein, OmpC, which is one of the major proteins in the outer membrane of Escherichia coli. In order to obtain structural information it was necessary to develop a suitable technique for preparing two-dimensional crystalline arrays of this membrane protein in an unfixed, unstained and hydrated condition. Electron micrographs were recorded at exposures of less than 5 electrons/A 2 in order to avoid severe radiation damage. The resulting images were crystallographically averaged, in order to overcome the statistical limitations associated with the low electron exposures. The resulting images, which extend to a resolution of approx. 13.5 A, lend themselves to a natural interpretation that is consistent with the mass density of protein, water and lipid, prior data from 2-D and 3-D structure studies of negatively stained specimens at approx. = 20 A resolution, and published spectroscopic data on the peptide chain secondary structure

  10. Clonality, outer-membrane proteins profile and efflux pump in KPC- producing Enterobacter sp. in Brazil.

    Science.gov (United States)

    Rosa, Juliana Ferraz; Rizek, Camila; Marchi, Ana Paula; Guimaraes, Thais; Miranda, Lourdes; Carrilho, Claudia; Levin, Anna S; Costa, Silvia F

    2017-03-17

    Carbapenems resistance in Enterobacter spp. has increased in the last decade, few studies, however, described the mechanisms of resistance in this bacterium. This study evaluated clonality and mechanisms of carbapenems resistance in clinical isolates of Enterobacter spp. identified in three hospitals in Brazil (Hospital A, B and C) over 7-year. Antibiotics sensitivity, pulsed-field gel electrophoresis (PFGE), PCR for carbapenemase and efflux pump genes were performed for all carbapenems-resistant isolates. Outer-membrane protein (OMP) was evaluated based on PFGE profile. A total of 130 isolates of Enterobacter spp were analyzed, 44/105 (41, 9%) E. aerogenes and 8/25 (32,0%) E. cloacae were resistant to carbapenems. All isolates were susceptible to fosfomycin, polymyxin B and tigecycline. KPC was present in 88.6% of E. aerogenes and in all E. cloacae resistant to carbapenems. The carbapenems-resistant E. aerogenes identified in hospital A belonged to six clones, however, a predominant clone was identified in this hospital over the study period. There is a predominant clone in Hospital B and Hospital C as well. The mechanisms of resistance to carbapenems differ among subtypes. Most of the isolates co-harbored blaKPC, blaTEM and /or blaCTX associated with decreased or lost of 35-36KDa and or 39 KDa OMP. The efflux pump AcrAB-TolC gene was only identified in carbapenems-resistant E. cloacae. There was a predominant clone in each hospital suggesting that cross-transmission of carbapenems-resistant Enterobacter spp. was frequent. The isolates presented multiple mechanisms of resistance to carbapenems including OMP alteration.

  11. The Annotation, Mapping, Expression and Network (AMEN suite of tools for molecular systems biology

    Directory of Open Access Journals (Sweden)

    Primig Michael

    2008-02-01

    Full Text Available Abstract Background High-throughput genome biological experiments yield large and multifaceted datasets that require flexible and user-friendly analysis tools to facilitate their interpretation by life scientists. Many solutions currently exist, but they are often limited to specific steps in the complex process of data management and analysis and some require extensive informatics skills to be installed and run efficiently. Results We developed the Annotation, Mapping, Expression and Network (AMEN software as a stand-alone, unified suite of tools that enables biological and medical researchers with basic bioinformatics training to manage and explore genome annotation, chromosomal mapping, protein-protein interaction, expression profiling and proteomics data. The current version provides modules for (i uploading and pre-processing data from microarray expression profiling experiments, (ii detecting groups of significantly co-expressed genes, and (iii searching for enrichment of functional annotations within those groups. Moreover, the user interface is designed to simultaneously visualize several types of data such as protein-protein interaction networks in conjunction with expression profiles and cellular co-localization patterns. We have successfully applied the program to interpret expression profiling data from budding yeast, rodents and human. Conclusion AMEN is an innovative solution for molecular systems biological data analysis freely available under the GNU license. The program is available via a website at the Sourceforge portal which includes a user guide with concrete examples, links to external databases and helpful comments to implement additional functionalities. We emphasize that AMEN will continue to be developed and maintained by our laboratory because it has proven to be extremely useful for our genome biological research program.

  12. The PAUL Suit(©) : an experience of ageing.

    Science.gov (United States)

    Bennett, Paul; Moore, Malcolm; Wenham, John

    2016-04-01

    An ageing population worldwide makes it increasingly important that health students understand issues that elderly people face and can provide empathic care to them. This teaching department in an isolated rural setting developed an interprofessional learning session to assist health students to understand issues of functional loss and social isolation that can affect elderly people. The Premature Ageing Unisex Leisure (PAUL) Suit(©) was developed as part of a 1-day learning session for undergraduate health students - including students of medicine, nursing and allied health - attending clinical placement in far-west New South Wales. The suit was developed locally and can be adjusted to simulate a wide range of functional losses in the wearer. Students undertake a range of daily tasks in the community while wearing the suit in the company of a student 'carer'. Over the past 4 years, approximately 140 students have participated in the simulation. Post-simulation evaluations report that students gain a greater understanding of some functional issues associated with ageing, and of the social isolation that can be associated with these. The experiential nature of the activity leads to some powerful insights. This activity is an innovative, experiential tool to deepen students understanding of issues related to ageing This activity is an innovative, experiential tool to deepen students understanding of issues relating to ageing. The interprofessional nature of the activity is an important factor in the success of the day, and produces a wide range of shared insights. The activity also enhances the partnerships between the university, the health service and the local community. Our experience supports the value of simulation in providing a deep learning opportunity in the area of ageing and disability. © 2015 John Wiley & Sons Ltd.

  13. pcircle - A Suite of Scalable Parallel File System Tools

    Energy Technology Data Exchange (ETDEWEB)

    2015-10-01

    Most of the software related to file system are written for conventional local file system, they are serialized and can't take advantage of the benefit of a large scale parallel file system. "pcircle" software builds on top of ubiquitous MPI in cluster computing environment and "work-stealing" pattern to provide a scalable, high-performance suite of file system tools. In particular - it implemented parallel data copy and parallel data checksumming, with advanced features such as async progress report, checkpoint and restart, as well as integrity checking.

  14. The Chernobyl cloud: comments on a non-suit

    International Nuclear Information System (INIS)

    2011-01-01

    This article comments the non-suit decision taken by a Paris court at the benefit of Pierre Pellerin after a trial about his declarations concerning the Chernobyl cloud. It recalls the great number of controls of radioactive contamination levels performed in France at this time by the SCRPI with Mr Pellerin at its head. It states that French authorities behaved like other European authorities with respect to the contamination brought by the cloud, that no epidemiological study has ever revealed pathologies which could be due to the cloud, and that the increase of cancers in Corsica is not proved

  15. OpenMP 4.5 Validation and Verification Suite

    Energy Technology Data Exchange (ETDEWEB)

    2017-12-15

    OpenMP, a directive-based programming API, introduce directives for accelerator devices that programmers are starting to use more frequently in production codes. To make sure OpenMP directives work correctly across architectures, it is critical to have a mechanism that tests for an implementation's conformance to the OpenMP standard. This testing process can uncover ambiguities in the OpenMP specification, which helps compiler developers and users make a better use of the standard. We fill this gap through our validation and verification test suite that focuses on the offload directives available in OpenMP 4.5.

  16. Chromite symplectites in Mg-suite troctolite 76535 as evidence for infiltration metasomatism of a lunar layered intrusion

    Science.gov (United States)

    Elardo, Stephen M.; McCubbin, Francis M.; Shearer, Charles K.

    2012-06-01

    Despite the very low chromium concentrations in its cumulus olivine (˜140 ppm), lunar troctolite 76535 contains large amounts of Cr sporadically, but highly concentrated, in symplectite assemblages consisting of Mg-Al-chromite and two pyroxenes. Previously proposed symplectite formation mechanisms include crystallization of trapped interstitial melt, diffusion of Cr from cumulus olivine, and/or remobilization of cumulus chromite grains. These mechanisms would imply that the highly Cr-depleted nature of Mg-suite parental magmas and their source materials inferred from cumulus olivine may be illusory. We have conducted a detailed petrologic and textural study of symplectites, as well as chromite veins, intercumulus assemblages, olivine-hosted melt inclusions and clinopyroxene-troilite veins in 76535 with the goals of constraining the origin of the symplectites, and the degree of Cr-depletion in Mg-suite magmas relative to other lunar basalts. Orthopyroxene and clinopyroxene in melt inclusions are depleted in Cr relative to their symplectite counterparts, averaging 900 and 1200 ppm vs. 7400 and 8100 ppm Cr2O3, respectively. Olivine in contact with symplectite assemblages may exhibit a diffusion profile of Cr going into olivine, whereas olivine boundaries away from symplectites show no diffusion profile. There is also a distinct lack of primary chromite as inclusions in cumulus phases and melt inclusions. Multiple textural observations, melt inclusion chemistry, and modeling of chromite-olivine equilibrium rule out previously proposed symplectite formation mechanisms, and strongly suggest that chromite was not a primary crystallization product of the 76535 parental magma. Accordingly, the post-cumulus addition of Cr and Fe is required to produce the symplectites. After considering multiple models, the addition of Cr and Fe to 76535 via infiltration metasomatism by an exogenous chromite-saturated melt is the model most consistent with multiple textural and geochemical

  17. Karolinske psychodynamic profile (KAPP)

    DEFF Research Database (Denmark)

    Mathiesen, Birgit Bork; Søgaard, Ulf

    2006-01-01

    psykologiske testmetoder, assesment, Karolinska psychodynamic profile (KAPP), psykodynamisk profil......psykologiske testmetoder, assesment, Karolinska psychodynamic profile (KAPP), psykodynamisk profil...

  18. Differential expression of in vivo and in vitro protein profile of outer membrane of Acidovorax avenae subsp. avenae.

    Directory of Open Access Journals (Sweden)

    Muhammad Ibrahim

    Full Text Available Outer membrane (OM proteins play a significant role in bacterial pathogenesis. In this work, we examined and compared the expression of the OM proteins of the rice pathogen Acidovorax avenae subsp. avenae strain RS-1, a Gram-negative bacterium, both in an in vitro culture medium and in vivo rice plants. Global proteomic profiling of A. avenae subsp. avenae strain RS-1 comparing in vivo and in vitro conditions revealed the differential expression of proteins affecting the survival and pathogenicity of the rice pathogen in host plants. The shotgun proteomics analysis of OM proteins resulted in the identification of 97 proteins in vitro and 62 proteins in vivo by mass spectrometry. Among these OM proteins, there is a high number of porins, TonB-dependent receptors, lipoproteins of the NodT family, ABC transporters, flagellins, and proteins of unknown function expressed under both conditions. However, the major proteins such as phospholipase and OmpA domain containing proteins were expressed in vitro, while the proteins such as the surface anchored protein F, ATP-dependent Clp protease, OmpA and MotB domain containing proteins were expressed in vivo. This may indicate that these in vivo OM proteins have roles in the pathogenicity of A. avenae subsp. avenae strain RS-1. In addition, the LC-MS/MS identification of OmpA and MotB validated the in silico prediction of the existance of Type VI secretion system core components. To the best of our knowledge, this is the first study to reveal the in vitro and in vivo protein profiles, in combination with LC-MS/MS mass spectra, in silico OM proteome and in silico genome wide analysis, of pathogenicity or plant host required proteins of a plant pathogenic bacterium.

  19. Differential expression of in vivo and in vitro protein profile of outer membrane of Acidovorax avenae subsp. avenae.

    Science.gov (United States)

    Ibrahim, Muhammad; Shi, Yu; Qiu, Hui; Li, Bin; Jabeen, Amara; Li, Liping; Liu, He; Kube, Michael; Xie, Guanlin; Wang, Yanli; Blondel, Carlos; Santiviago, Carlos A; Contreras, Ines; Sun, Guochang

    2012-01-01

    Outer membrane (OM) proteins play a significant role in bacterial pathogenesis. In this work, we examined and compared the expression of the OM proteins of the rice pathogen Acidovorax avenae subsp. avenae strain RS-1, a Gram-negative bacterium, both in an in vitro culture medium and in vivo rice plants. Global proteomic profiling of A. avenae subsp. avenae strain RS-1 comparing in vivo and in vitro conditions revealed the differential expression of proteins affecting the survival and pathogenicity of the rice pathogen in host plants. The shotgun proteomics analysis of OM proteins resulted in the identification of 97 proteins in vitro and 62 proteins in vivo by mass spectrometry. Among these OM proteins, there is a high number of porins, TonB-dependent receptors, lipoproteins of the NodT family, ABC transporters, flagellins, and proteins of unknown function expressed under both conditions. However, the major proteins such as phospholipase and OmpA domain containing proteins were expressed in vitro, while the proteins such as the surface anchored protein F, ATP-dependent Clp protease, OmpA and MotB domain containing proteins were expressed in vivo. This may indicate that these in vivo OM proteins have roles in the pathogenicity of A. avenae subsp. avenae strain RS-1. In addition, the LC-MS/MS identification of OmpA and MotB validated the in silico prediction of the existance of Type VI secretion system core components. To the best of our knowledge, this is the first study to reveal the in vitro and in vivo protein profiles, in combination with LC-MS/MS mass spectra, in silico OM proteome and in silico genome wide analysis, of pathogenicity or plant host required proteins of a plant pathogenic bacterium.

  20. Anesthesia and the pediatric cardiac catheterization suite: a review.

    Science.gov (United States)

    Lam, Jennifer E; Lin, Erica P; Alexy, Ryan; Aronson, Lori A

    2015-02-01

    Advances in technology over the last couple of decades have caused a shift in pediatric cardiac catheterization from a primary focus on diagnostics to innovative therapeutic interventions. These improvements allow patients a wider range of nonsurgical options for treatment of congenital heart disease. However, these therapeutic modalities can entail higher risk in an already complex patient population, compounded by the added challenges inherent to the environment of the cardiac catheterization suite. Anesthesiologists caring for children with congenital heart disease must understand not only the pathophysiology of the disease but also the effects the anesthetics and interventions have on the patient in order to provide a safe perioperative course. It is the aim of this article to review the latest catheterization modalities offered to patients with congenital heart disease, describe the unique challenges presented in the cardiac catheterization suite, list the most common complications encountered during catheterization and finally, to review the literature regarding different anesthetic drugs used in the catheterization lab. © 2014 John Wiley & Sons Ltd.

  1. SSAGES: Software Suite for Advanced General Ensemble Simulations

    Science.gov (United States)

    Sidky, Hythem; Colón, Yamil J.; Helfferich, Julian; Sikora, Benjamin J.; Bezik, Cody; Chu, Weiwei; Giberti, Federico; Guo, Ashley Z.; Jiang, Xikai; Lequieu, Joshua; Li, Jiyuan; Moller, Joshua; Quevillon, Michael J.; Rahimi, Mohammad; Ramezani-Dakhel, Hadi; Rathee, Vikramjit S.; Reid, Daniel R.; Sevgen, Emre; Thapar, Vikram; Webb, Michael A.; Whitmer, Jonathan K.; de Pablo, Juan J.

    2018-01-01

    Molecular simulation has emerged as an essential tool for modern-day research, but obtaining proper results and making reliable conclusions from simulations requires adequate sampling of the system under consideration. To this end, a variety of methods exist in the literature that can enhance sampling considerably, and increasingly sophisticated, effective algorithms continue to be developed at a rapid pace. Implementation of these techniques, however, can be challenging for experts and non-experts alike. There is a clear need for software that provides rapid, reliable, and easy access to a wide range of advanced sampling methods and that facilitates implementation of new techniques as they emerge. Here we present SSAGES, a publicly available Software Suite for Advanced General Ensemble Simulations designed to interface with multiple widely used molecular dynamics simulations packages. SSAGES allows facile application of a variety of enhanced sampling techniques—including adaptive biasing force, string methods, and forward flux sampling—that extract meaningful free energy and transition path data from all-atom and coarse-grained simulations. A noteworthy feature of SSAGES is a user-friendly framework that facilitates further development and implementation of new methods and collective variables. In this work, the use of SSAGES is illustrated in the context of simple representative applications involving distinct methods and different collective variables that are available in the current release of the suite. The code may be found at: https://github.com/MICCoM/SSAGES-public.

  2. STS-93 Pilot Ashby suits up before launch

    Science.gov (United States)

    1999-01-01

    In the Operations and Checkout Building during final launch preparations for the second time, STS-93 Pilot Jeffrey S. Ashby waves after donning his launch and entry suit while a suit tech adjusts his boot. After Space Shuttle Columbia's July 20 launch attempt was scrubbed at the T-7 second mark in the countdown, the launch was rescheduled for Thursday, July 22, at 12:28 a.m. EDT. The target landing date is July 26, 1999, at 11:24 p.m. EDT. STS- 93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The new telescope is 20 to 50 times more sensitive than any previous X-ray telescope and is expected unlock the secrets of supernovae, quasars and black holes. The STS-93 crew numbers five: Commander Eileen M. Collins, Ashby, and Mission Specialists Stephen A. Hawley (Ph.D.), Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  3. STS-93 M.S. Hawley suits up for launch

    Science.gov (United States)

    1999-01-01

    During final launch preparations in the Operations and Checkout Building, STS-93 Mission Specialist Steven A. Hawley (Ph.D.)gets help donning his launch and entry suit from a suit tech. After Space Shuttle Columbia's July 20 launch attempt was scrubbed at the T-7 second mark in the countdown, the launch was rescheduled for Thursday, July 22, at 12:28 a.m. EDT. The target landing date is July 26, 1999, at 11:24 p.m. EDT. STS-93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The new telescope is 20 to 50 times more sensitive than any previous X- ray telescope and is expected unlock the secrets of supernovae, quasars and black holes. The STS-93 crew numbers five: Commander Eileen M. Collins, Pilot Jeffrey S. Ashby, and Mission Specialists Hawley, Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  4. SSAGES: Software Suite for Advanced General Ensemble Simulations

    Energy Technology Data Exchange (ETDEWEB)

    Sidky, Hythem [Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, USA; Colón, Yamil J. [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Institute for Molecular Engineering and Materials Science Division, Argonne National Laboratory, Lemont, Illinois 60439, USA; Helfferich, Julian [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Steinbuch Center for Computing, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany; Sikora, Benjamin J. [Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, USA; Bezik, Cody [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Chu, Weiwei [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Giberti, Federico [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Guo, Ashley Z. [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Jiang, Xikai [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Lequieu, Joshua [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Li, Jiyuan [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Moller, Joshua [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Quevillon, Michael J. [Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, USA; Rahimi, Mohammad [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Ramezani-Dakhel, Hadi [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois 60637, USA; Rathee, Vikramjit S. [Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, USA; Reid, Daniel R. [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Sevgen, Emre [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Thapar, Vikram [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Webb, Michael A. [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Institute for Molecular Engineering and Materials Science Division, Argonne National Laboratory, Lemont, Illinois 60439, USA; Whitmer, Jonathan K. [Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, Indiana 46556, USA; de Pablo, Juan J. [Institute for Molecular Engineering, University of Chicago, Chicago, Illinois 60637, USA; Institute for Molecular Engineering and Materials Science Division, Argonne National Laboratory, Lemont, Illinois 60439, USA

    2018-01-28

    Molecular simulation has emerged as an essential tool for modern-day research, but obtaining proper results and making reliable conclusions from simulations requires adequate sampling of the system under consideration. To this end, a variety of methods exist in the literature that can enhance sampling considerably, and increasingly sophisticated, effective algorithms continue to be developed at a rapid pace. Implementation of these techniques, however, can be challenging for experts and non-experts alike. There is a clear need for software that provides rapid, reliable, and easy access to a wide range of advanced sampling methods, and that facilitates implementation of new techniques as they emerge. Here we present SSAGES, a publicly available Software Suite for Advanced General Ensemble Simulations designed to interface with multiple widely used molecular dynamics simulations packages. SSAGES allows facile application of a variety of enhanced sampling techniques—including adaptive biasing force, string methods, and forward flux sampling—that extract meaningful free energy and transition path data from all-atom and coarse grained simulations. A noteworthy feature of SSAGES is a user-friendly framework that facilitates further development and implementation of new methods and collective variables. In this work, the use of SSAGES is illustrated in the context of simple representative applications involving distinct methods and different collective variables that are available in the current release of the suite.

  5. Tier-3 Monitoring Software Suite (T3MON) proposal

    CERN Document Server

    Andreeva, J; The ATLAS collaboration; Klimentov, A; Korenkov, V; Oleynik, D; Panitkin, S; Petrosyan, A

    2011-01-01

    The ATLAS Distributed Computing activities concentrated so far in the “central” part of the computing system of the experiment, namely the first 3 tiers (CERN Tier0, the 10 Tier1s centres and the 60+ Tier2s). This is a coherent system to perform data processing and management on a global scale and host (re)processing, simulation activities down to group and user analysis. Many ATLAS Institutes and National Communities built (or have plans to build) Tier-3 facilities. The definition of Tier-3 concept has been outlined (REFERENCE). Tier-3 centres consist of non-pledged resources mostly dedicated for the data analysis by the geographically close or local scientific groups. Tier-3 sites comprise a range of architectures and many do not possess Grid middleware, which would render application of Tier-2 monitoring systems useless. This document describes a strategy to develop a software suite for monitoring of the Tier3 sites. This software suite will enable local monitoring of the Tier3 sites and the global vie...

  6. Wireless hydrotherapy smart suit for monitoring handicapped people

    Science.gov (United States)

    Correia, Jose H.; Mendes, Paulo M.

    2005-02-01

    This paper presents a smart suit, water impermeable, containing sensors and electronics for monitoring handicapped people at hydrotherapy sessions in swimming-pools. For integration into textiles, electronic components should be designed in a functional, robust and inexpensive way. Therefore, small-size electronics microsystems are a promising approach. The smart suit allows the monitoring of individual biometric data, such as heart rate, temperature and movement of the body. Two solutions for transmitting the data wirelessly are presented: through a low-voltage (3.0 V), low-power, CMOS RF IC (1.6 mm x 1.5 mm size dimensions) operating at 433 MHz, with ASK modulation and a patch antenna built on lossy substrates compatible with integrated circuits fabrication. Two different substrates were used for antenna implementation: high-resistivity silicon (HRS) and Corning Pyrex #7740 glass. The antenna prototypes were built to operate close to the 5 GHz ISM band. They operate at a center frequency of 5.705 GHz (HRS) and 5.995 GHz (Pyrex). The studied parameters were: substrate thickness, substrate losses, oxide thickness, metal conductivity and thickness. The antenna on HRS uses an area of 8 mm2, providing a 90 MHz bandwidth and ~0.3 dBi of gain. On a glass substrate, the antenna uses 12 mm2, provides 100 MHz bandwidth and ~3 dBi of gain.

  7. A comparison of two Shuttle launch and entry suits - Reach envelope, isokinetic strength, and treadmill tests

    Science.gov (United States)

    Schafer, Lauren E.; Rajulu, Sudhakar L.; Klute, Glenn K.

    1992-01-01

    A quantification has been conducted of any existing differences between the performance, in operational conditions, of the Space Shuttle crew Launch Entry Suit (LES) and the new Advanced Crew Escape Suit (ACES). While LES is a partial-pressure suit, the ACES system which is being considered as a replacement for LES is a full-pressure suit. Three tests have been conducted with six subjects to ascertain the suits' reach envelope, strength, and treadmill performance. No significant operational differences were found between the two suit designs.

  8. Architecture of a consent management suite and integration into IHE-based Regional Health Information Networks.

    Science.gov (United States)

    Heinze, Oliver; Birkle, Markus; Köster, Lennart; Bergh, Björn

    2011-10-04

    The University Hospital Heidelberg is implementing a Regional Health Information Network (RHIN) in the Rhine-Neckar-Region in order to establish a shared-care environment, which is based on established Health IT standards and in particular Integrating the Healthcare Enterprise (IHE). Similar to all other Electronic Health Record (EHR) and Personal Health Record (PHR) approaches the chosen Personal Electronic Health Record (PEHR) architecture relies on the patient's consent in order to share documents and medical data with other care delivery organizations, with the additional requirement that the German legislation explicitly demands a patients' opt-in and does not allow opt-out solutions. This creates two issues: firstly the current IHE consent profile does not address this approach properly and secondly none of the employed intra- and inter-institutional information systems, like almost all systems on the market, offers consent management solutions at all. Hence, the objective of our work is to develop and introduce an extensible architecture for creating, managing and querying patient consents in an IHE-based environment. Based on the features offered by the IHE profile Basic Patient Privacy Consent (BPPC) and literature, the functionalities and components to meet the requirements of a centralized opt-in consent management solution compliant with German legislation have been analyzed. Two services have been developed and integrated into the Heidelberg PEHR. The standard-based Consent Management Suite consists of two services. The Consent Management Service is able to receive and store consent documents. It can receive queries concerning a dedicated patient consent, process it and return an answer. It represents a centralized policy enforcement point. The Consent Creator Service allows patients to create their consents electronically. Interfaces to a Master Patient Index (MPI) and a provider index allow to dynamically generate XACML-based policies which are

  9. Enhanced Verification Test Suite for Physics Simulation Codes

    Energy Technology Data Exchange (ETDEWEB)

    Kamm, J R; Brock, J S; Brandon, S T; Cotrell, D L; Johnson, B; Knupp, P; Rider, W; Trucano, T; Weirs, V G

    2008-10-10

    This document discusses problems with which to augment, in quantity and in quality, the existing tri-laboratory suite of verification problems used by Los Alamos National Laboratory (LANL), Lawrence Livermore National Laboratory (LLNL), and Sandia National Laboratories (SNL). The purpose of verification analysis is demonstrate whether the numerical results of the discretization algorithms in physics and engineering simulation codes provide correct solutions of the corresponding continuum equations. The key points of this document are: (1) Verification deals with mathematical correctness of the numerical algorithms in a code, while validation deals with physical correctness of a simulation in a regime of interest. This document is about verification. (2) The current seven-problem Tri-Laboratory Verification Test Suite, which has been used for approximately five years at the DOE WP laboratories, is limited. (3) Both the methodology for and technology used in verification analysis have evolved and been improved since the original test suite was proposed. (4) The proposed test problems are in three basic areas: (a) Hydrodynamics; (b) Transport processes; and (c) Dynamic strength-of-materials. (5) For several of the proposed problems we provide a 'strong sense verification benchmark', consisting of (i) a clear mathematical statement of the problem with sufficient information to run a computer simulation, (ii) an explanation of how the code result and benchmark solution are to be evaluated, and (iii) a description of the acceptance criterion for simulation code results. (6) It is proposed that the set of verification test problems with which any particular code be evaluated include some of the problems described in this document. Analysis of the proposed verification test problems constitutes part of a necessary--but not sufficient--step that builds confidence in physics and engineering simulation codes. More complicated test cases, including physics models of

  10. A suite of standards for radiation monitors and their revisions

    International Nuclear Information System (INIS)

    Noda, Kimio

    1991-01-01

    A suite of standards for radiation monitors applied in nuclear facilities in Japan was compiled mainly by Health Physicists in Power Reactor and Nuclear Fuel Development (PNC) and Japan Atomic Energy Research Institute (JAERI), and issued in 1971 as 'The Standard for Radiation Monitors'. PNC facilities such as Reprocessing Plant and Plutonium Fuel Fabrication Facility, as well as other nuclear industries have applied the standard, and contributed improvement of practical maintenability and availability of the radiation monitors. Meanwhile, the radiation monitors have remarkably progressed in its application and size of the monitors is growing. Furthermore, manufacturing techniques have significantly progressed especially in the field of system concepts and electronics elements. These progresses require revision of the standards. 'The Standard for Radiation Monitors' has been revised considering the problems in practical application and data processing capability. Considerations are given to keep compatibility of old and new modules. (author)

  11. User Guide for the STAYSL PNNL Suite of Software Tools

    Energy Technology Data Exchange (ETDEWEB)

    Greenwood, Lawrence R.; Johnson, Christian D.

    2013-02-27

    The STAYSL PNNL software suite provides a set of tools for working with neutron activation rates measured in a nuclear fission reactor, an accelerator-based neutron source, or any neutron field to determine the neutron flux spectrum through a generalized least-squares approach. This process is referred to as neutron spectral adjustment since the preferred approach is to use measured data to adjust neutron spectra provided by neutron physics calculations. The input data consist of the reaction rates based on measured activities, an initial estimate of the neutron flux spectrum, neutron activation cross sections and their associated uncertainties (covariances), and relevant correction factors. The output consists of the adjusted neutron flux spectrum and associated covariance matrix, which is useful for neutron dosimetry and radiation damage calculations.

  12. Court rules against failed viatical firm in investor suit.

    Science.gov (United States)

    1999-10-01

    A Federal appeals court has revived a claim against Dignity Partners Inc., a viatical business, and offshoot of a financial-services firm. Dignity Partners operated by buying the life insurance policies of terminally ill people. The company was charged with making false and misleading statements in its prospectus for an initial public stock offering. Five months later, the company announced that it would not accept new customers with AIDS, a group which represented 95 percent of its accounts at that time. The company had information from researchers and clinicians that the introduction of protease inhibitors would greatly increase life expectancy for its customers and would reduce company profits. This information was not generally available to potential investors. The suit against the company alleges violations of the Securities Act of 1933 and the Exchange Act of 1934, both which govern stock trading.

  13. STS-93 Commander Collins suits up for launch

    Science.gov (United States)

    1999-01-01

    During the third launch preparations in the Operations and Checkout Building, STS-93 Commander Eileen M. Collins waves while having her launch and entry suit checked. After Space Shuttle Columbia's July 20 and 22 launch attempts were scrubbed, the launch was again rescheduled for Friday, July 23, at 12:24 a.m. EDT. STS-93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The STS-93 crew numbers five: Commander Collins, Pilot Jeffrey S. Ashby, and Mission Specialists Stephen A. Hawley (Ph.D.), Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  14. STS-93 Mission Specialist Cady Coleman suits up for launch

    Science.gov (United States)

    1999-01-01

    For the third time, during final launch preparations in the Operations and Checkout Building, STS-93 Mission Specialist Catherine G. Coleman (Ph.D.) dons her launch and entry suit. After Space Shuttle Columbia's July 20 and 22 launch attempts were scrubbed, the launch was again rescheduled for Friday, July 23, at 12:24 a.m. EDT. STS-93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The STS-93 crew numbers five: Commander Eileen M. Collins, Pilot Jeffrey S. Ashby, and Mission Specialists Stephen A. Hawley (Ph.D.), Coleman, and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  15. STS-93 Commander Eileen Collins suits up for launch

    Science.gov (United States)

    1999-01-01

    For the third time, in the Operations and Checkout Building, STS- 93 Commander Eileen M. Collins tries on her helmet with her launch and entry suit. After Space Shuttle Columbia's July 20 and 22 launch attempts were scrubbed, the launch was again rescheduled for Friday, July 23, at 12:24 a.m. EDT. STS-93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The STS-93 crew numbers five: Commander Collins, Pilot Jeffrey S. Ashby, and Mission Specialists Stephen A. Hawley (Ph.D.), Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  16. STS-93 Mission Specialist Hawley suits up for launch

    Science.gov (United States)

    1999-01-01

    For the third time, during final launch preparations in the Operations and Checkout Building, STS-93 Mission Specialist Steven A. Hawley (Ph.D.) waves after donning his launch and entry suit. After Space Shuttle Columbia's July 20 and 22 launch attempts were scrubbed, the launch was again rescheduled for Friday, July 23, at 12:24 a.m. EDT. STS-93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The STS-93 crew numbers five: Commander Eileen M. Collins, Pilot Jeffrey S. Ashby, and Mission Specialists Hawley, Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  17. STS-93 Pilot Ashby suits up for launch

    Science.gov (United States)

    1999-01-01

    In the Operations and Checkout Building during final launch preparations for the third time, STS-93 Pilot Jeffrey S. Ashby pulls on his glove, part of his launch and entry suit. After Space Shuttle Columbia's July 20 and 22 launch attempts were scrubbed, the launch was again rescheduled for Friday, July 23, at 12:24 a.m. EDT. STS-93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The STS-93 crew numbers five: Commander Eileen Collins, Ashby, and Mission Specialists Stephen A. Hawley (Ph.D.), Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  18. STS-92 Pilot Pam Melroy suits up for launch

    Science.gov (United States)

    2000-01-01

    In the Operations and Checkout Building, STS-92 Pilot Pamela Ann Melroy smiles during suit check before heading out to the Astrovan for the ride to Launch Pad 39A. During the 11-day mission to the International Space Station, four extravehicular activities (EVAs), or spacewalks, are planned for construction. The payload includes the Integrated Truss Structure Z-1 and the third Pressurized Mating Adapter. The Z-1 truss is the first of 10 that will become the backbone of the Space Station, eventually stretching the length of a football field. PMA-3 will provide a Shuttle docking port for solar array installation on the sixth Station flight and Lab installation on the seventh Station flight. Launch is scheduled for 7:17 p.m. EDT. Landing is expected Oct. 22 at 2:10 p.m. EDT.

  19. The Characterization of Biosignatures in Caves Using an Instrument Suite

    Science.gov (United States)

    Uckert, Kyle; Chanover, Nancy J.; Getty, Stephanie; Voelz, David G.; Brinckerhoff, William B.; McMillan, Nancy; Xiao, Xifeng; Boston, Penelope J.; Li, Xiang; McAdam, Amy; Glenar, David A.; Chavez, Arriana

    2017-12-01

    The search for life and habitable environments on other Solar System bodies is a major motivator for planetary exploration. Due to the difficulty and significance of detecting extant or extinct extraterrestrial life in situ, several independent measurements from multiple instrument techniques will bolster the community's confidence in making any such claim. We demonstrate the detection of subsurface biosignatures using a suite of instrument techniques including IR reflectance spectroscopy, laser-induced breakdown spectroscopy, and scanning electron microscopy/energy dispersive X-ray spectroscopy. We focus our measurements on subterranean calcium carbonate field samples, whose biosignatures are analogous to those that might be expected on some high-interest astrobiology targets. In this work, we discuss the feasibility and advantages of using each of the aforementioned instrument techniques for the in situ search for biosignatures and present results on the autonomous characterization of biosignatures using multivariate statistical analysis techniques.

  20. Comparing apples and oranges: the Community Intercomparison Suite

    Science.gov (United States)

    Schutgens, Nick; Stier, Philip; Kershaw, Philip; Pascoe, Stephen

    2015-04-01

    Visual representation and comparison of geoscientific datasets presents a huge challenge due to the large variety of file formats and spatio-temporal sampling of data (be they observations or simulations). The Community Intercomparison Suite attempts to greatly simplify these tasks for users by offering an intelligent but simple command line tool for visualisation and colocation of diverse datasets. In addition, CIS can subset and aggregate large datasets into smaller more manageable datasets. Our philosophy is to remove as much as possible the need for specialist knowledge by the user of the structure of a dataset. The colocation of observations with model data is as simple as: "cis col ::" which will resample the simulation data to the spatio-temporal sampling of the observations, contingent on a few user-defined options that specify a resampling kernel. As an example, we apply CIS to a case study of biomass burning aerosol from the Congo. Remote sensing observations, in-situe observations and model data are shown in various plots, with the purpose of either comparing different datasets or integrating them into a single comprehensive picture. CIS can deal with both gridded and ungridded datasets of 2, 3 or 4 spatio-temporal dimensions. It can handle different spatial coordinates (e.g. longitude or distance, altitude or pressure level). CIS supports both HDF, netCDF and ASCII file formats. The suite is written in Python with entirely publicly available open source dependencies. Plug-ins allow a high degree of user-moddability. A web-based developer hub includes a manual and simple examples. CIS is developed as open source code by a specialist IT company under supervision of scientists from the University of Oxford and the Centre of Environmental Data Archival as part of investment in the JASMIN superdatacluster facility.

  1. Automated integration of lidar into the LANDFIRE product suite

    Science.gov (United States)

    Peterson, Birgit; Nelson, Kurtis; Seielstad, Carl; Stoker, Jason M.; Jolly, W. Matt; Parsons, Russell

    2015-01-01

    Accurate information about three-dimensional canopy structure and wildland fuel across the landscape is necessary for fire behaviour modelling system predictions. Remotely sensed data are invaluable for assessing these canopy characteristics over large areas; lidar data, in particular, are uniquely suited for quantifying three-dimensional canopy structure. Although lidar data are increasingly available, they have rarely been applied to wildland fuels mapping efforts, mostly due to two issues. First, the Landscape Fire and Resource Planning Tools (LANDFIRE) program, which has become the default source of large-scale fire behaviour modelling inputs for the US, does not currently incorporate lidar data into the vegetation and fuel mapping process because spatially continuous lidar data are not available at the national scale. Second, while lidar data are available for many land management units across the US, these data are underutilized for fire behaviour applications. This is partly due to a lack of local personnel trained to process and analyse lidar data. This investigation addresses these issues by developing the Creating Hybrid Structure from LANDFIRE/lidar Combinations (CHISLIC) tool. CHISLIC allows individuals to automatically generate a suite of vegetation structure and wildland fuel parameters from lidar data and infuse them into existing LANDFIRE data sets. CHISLIC will become available for wider distribution to the public through a partnership with the U.S. Forest Service’s Wildland Fire Assessment System (WFAS) and may be incorporated into the Wildland Fire Decision Support System (WFDSS) with additional design and testing. WFAS and WFDSS are the primary systems used to support tactical and strategic wildland fire management decisions.

  2. Space Suit Simulator (S3) for Partial Gravity EVA Experimentation and Training, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Pressurized space suits impose high joint torques on the wearer, reducing mobility for upper and lower body motions. Using actual space suits in training or...

  3. Astronaut Neil Armstrong in Launch Complex 16 trailer during suiting up

    Science.gov (United States)

    1966-01-01

    Astronaut Neil A. Armstrong, command pilot of the Gemini 8 space flight, sits in the Launch Complex 16 trailer during suiting up operations for the Gemini 8 mission. Suit technician Jim Garrepy assists.

  4. Development of an online men’s suits customizing system using heuristic procedure for wheelchair users

    NARCIS (Netherlands)

    Jeong, Minseok; Yang, Chuneun; You, Heecheon; Park, Kwangae; Lee, W.

    2016-01-01

    An online suit-customizing system for the special accessibility needs of wheelchair users should be developed because the demand for business suits by wheelchair users involved in economic activities has increased. This study
    develops a user interface an online customizing system for men's suits

  5. A Lactococcus lactis BFE920 feed vaccine expressing a fusion protein composed of the OmpA and FlgD antigens from Edwardsiella tarda was significantly better at protecting olive flounder (Paralichthys olivaceus) from edwardsiellosis than single antigen vaccines.

    Science.gov (United States)

    Beck, Bo Ram; Lee, Soon Ho; Kim, Daniel; Park, Ji Hye; Lee, Hyun Kyung; Kwon, San-Sung; Lee, Kwan Hee; Lee, Jae Il; Song, Seong Kyu

    2017-09-01

    Edwardsiellosis is a major fish disease that causes a significant economic damage in the aquaculture industry. Here, we assessed vaccine efficacy after feeding oral vaccines to olive flounder (Paralichthys olivaceus), either L. lactis BFE920 expressing Edwardsiella tarda outer membrane protein A (OmpA), flagellar hook protein D (FlgD), or a fusion antigen of the two. Feed vaccination was done twice with a one-week interval. Fish were fed regular feed adsorbed with the vaccines. Feed vaccination was given over the course of one week to maximize the interaction between the feed vaccines and the fish intestine. Flounder fed the vaccine containing the fusion antigen had significantly elevated levels T cell genes (CD4-1, CD4-2, and CD8α), type 1 helper T cell (Th1) subset indicator genes (T-bet and IFN-γ), and antigen-specific antibodies compared to the groups fed the single antigen-expressing vaccines. Furthermore, the superiority of the fusion vaccine was also observed in survival rates when fish were challenged with E. tarda: OmpA-FlgD-expressing vaccine (82.5% survival); FlgD-vaccine (55.0%); OmpA-vaccine (50%); WT L. lactis BFE920 (37.5%); Ctrl (10%). In addition, vaccine-fed fish exhibited increased weight gain (∼20%) and a decreased feed conversion ratio (∼20%) during the four week vaccination period. Flounder fed the FlgD-expressing vaccine, either the single or the fusion form, had significantly increased expression of TLR5M, IL-1β, and IL-12p40, suggesting that the FlgD may be a ligand of olive flounder TLR5M receptor or closely related to the TLR5M pathway. In conclusion, the present study demonstrated that olive flounder fed L. lactis BFE920 expressing a fusion antigen composed of E. tarda OmpA and FlgD showed a strong protective effect against edwardsiellosis indicating this may be developed as an E. tarda feed vaccine. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Using Piezoelectric Ceramics for Dust Mitigation of Space Suits

    Science.gov (United States)

    Angel, Heather K.

    2004-01-01

    The particles that make up moon dust and Mars soil can be hazardous to an astronaut s health if not handled properly. In the near future, while exploring outer space, astronauts plan to wander the surfaces of unknown planets. During these explorations, dust and soil will cling to their space suits and become imbedded in the fabric. The astronauts will track moon dust and mars soil back into their living quarters. This not only will create a mess with millions of tiny air-born particles floating around, but will also be dangerous in the case that the fine particles are breathed in and become trapped in an astronaut s lungs. research center are investigating ways to remove these particles from space suits. This problem is very difficult due to the nature of the particles: They are extremely small and have jagged edges which can easily latch onto the fibers of the fabric. For the past summer, I have been involved in researching the potential problems, investigating ways to remove the particles, and conducting experiments to validate the techniques. The current technique under investigation uses piezoelectric ceramics imbedded in the fabric that vibrate and shake the particles free. The particles will be left on the planet s surface or collected a vacuum to be disposed of later. The ceramics vibrate when connected to an AC voltage supply and create a small scale motion similar to what people use at the beach to shake sand off of a beach towel. Because the particles are so small, similar to volcanic ash, caution must be taken to make sure that this technique does not further inbed them in the fabric and make removal more difficult. Only a very precise range of frequency and voltage will produce a suitable vibration. My summer project involved many experiments to determine the correct range. Analysis involved hands on experience with oscilloscopes, amplifiers, piezoelectrics, a high speed camera, microscopes and computers. perfect this technology. Someday, vibration to

  7. Philosophies Applied in the Selection of Space Suit Joint Range of Motion Requirements

    Science.gov (United States)

    Aitchison, Lindsway; Ross, Amy; Matty, Jennifer

    2009-01-01

    Space suits are the most important tool for astronauts working in harsh space and planetary environments; suits keep crewmembers alive and allow them to perform exploration, construction, and scientific tasks on a routine basis over a period of several months. The efficiency with which the tasks are performed is largely dictated by the mobility features of the space suit. For previous space suit development programs, the mobility requirements were written as pure functional mobility requirements that did not separate joint ranges of motion from the joint torques. The Constellation Space Suit Element has the goal to make more quantitative mobility requirements that focused on the individual components of mobility to enable future suit designers to build and test systems more effectively. This paper details the test planning and selection process for the Constellation space suit pressure garment range of motion requirements.

  8. Development of an advanced rocket propellant handler's suit

    Science.gov (United States)

    Doerr, D. F.

    2001-01-01

    Most launch vehicles and satellites in the US inventory rely upon the use of hypergolic rocket propellants, many of which are toxic to humans. These fuels and oxidizers, such as hydrazine and nitrogen tetroxide have threshold limit values as low as 0.01 PPM. It is essential to provide space workers handling these agents whole body protection as they are universally hazardous not only to the respiratory system, but the skin as well. This paper describes a new method for powering a whole body protective garment to assure the safety of ground servicing crews. A new technology has been developed through the small business innovative research program at the Kennedy Space Center. Currently, liquid air is used in the environmental control unit (ECU) that powers the propellant handlers suit (PHE). However, liquid air exhibits problems with attitude dependence, oxygen enrichment, and difficulty with reliable quantity measurement. The new technology employs the storage of the supply air as a supercritical gas. This method of air storage overcomes all of three problems above while maintaining high density storage at relatively low vessel pressures (rights reserved.

  9. Advanced Space Suit Portable Life Support Subsystem Packaging Design

    Science.gov (United States)

    Howe, Robert; Diep, Chuong; Barnett, Bob; Thomas, Gretchen; Rouen, Michael; Kobus, Jack

    2006-01-01

    This paper discusses the Portable Life Support Subsystem (PLSS) packaging design work done by the NASA and Hamilton Sundstrand in support of the 3 future space missions; Lunar, Mars and zero-g. The goal is to seek ways to reduce the weight of PLSS packaging, and at the same time, develop a packaging scheme that would make PLSS technology changes less costly than the current packaging methods. This study builds on the results of NASA s in-house 1998 study, which resulted in the "Flex PLSS" concept. For this study the present EMU schematic (low earth orbit) was used so that the work team could concentrate on the packaging. The Flex PLSS packaging is required to: protect, connect, and hold the PLSS and its components together internally and externally while providing access to PLSS components internally for maintenance and for technology change without extensive redesign impact. The goal of this study was two fold: 1. Bring the advanced space suit integrated Flex PLSS concept from its current state of development to a preliminary design level and build a proof of concept mockup of the proposed design, and; 2. "Design" a Design Process, which accommodates both the initial Flex PLSS design and the package modifications, required to accommodate new technology.

  10. STS-95 Mission Specialist Duque suits up during TCDT

    Science.gov (United States)

    1998-01-01

    STS-95 Mission Specialist Pedro Duque of Spain, representing the European Space Agency, suits up in the Operations and Checkout Building prior to his trip to Launch Pad 39-B. Duque and the rest of the STS-95 crew are at KSC to participate in the Terminal Countdown Demonstration Test (TCDT) which includes mission familiarization activities, emergency egress training, and a simulated main engine cutoff. The other crew members are Payload Specialist Chiaki Mukai (M.D., Ph.D.), representing the National Space Development Agency of Japan (NASDA), Pilot Steven W. Lindsey, Mission Specialist Scott E. Parazynski, Mission Specialist Stephen K. Robinson, Payload Specialist John H. Glenn Jr., senator from Ohio, and Mission Commander Curtis L. Brown. The STS-95 mission, targeted for liftoff on Oct. 29, includes research payloads such as the Spartan solar-observing deployable spacecraft, the Hubble Space Telescope Orbital Systems Test Platform, the International Extreme Ultraviolet Hitchhiker, as well as the SPACEHAB single module with experiments on space flight and the aging process. Following the TCDT, the crew will be returning to Houston for final flight preparations.

  11. SECIMTools: a suite of metabolomics data analysis tools.

    Science.gov (United States)

    Kirpich, Alexander S; Ibarra, Miguel; Moskalenko, Oleksandr; Fear, Justin M; Gerken, Joseph; Mi, Xinlei; Ashrafi, Ali; Morse, Alison M; McIntyre, Lauren M

    2018-04-20

    Metabolomics has the promise to transform the area of personalized medicine with the rapid development of high throughput technology for untargeted analysis of metabolites. Open access, easy to use, analytic tools that are broadly accessible to the biological community need to be developed. While technology used in metabolomics varies, most metabolomics studies have a set of features identified. Galaxy is an open access platform that enables scientists at all levels to interact with big data. Galaxy promotes reproducibility by saving histories and enabling the sharing workflows among scientists. SECIMTools (SouthEast Center for Integrated Metabolomics) is a set of Python applications that are available both as standalone tools and wrapped for use in Galaxy. The suite includes a comprehensive set of quality control metrics (retention time window evaluation and various peak evaluation tools), visualization techniques (hierarchical cluster heatmap, principal component analysis, modular modularity clustering), basic statistical analysis methods (partial least squares - discriminant analysis, analysis of variance, t-test, Kruskal-Wallis non-parametric test), advanced classification methods (random forest, support vector machines), and advanced variable selection tools (least absolute shrinkage and selection operator LASSO and Elastic Net). SECIMTools leverages the Galaxy platform and enables integrated workflows for metabolomics data analysis made from building blocks designed for easy use and interpretability. Standard data formats and a set of utilities allow arbitrary linkages between tools to encourage novel workflow designs. The Galaxy framework enables future data integration for metabolomics studies with other omics data.

  12. Astronaut Anna Fisher Suits Up for NBS Training

    Science.gov (United States)

    1980-01-01

    The Hubble Space Telescope (HST) is a cooperative program of the European Space Agency (ESA) and the National Aeronautical and Space Administration (NASA) to operate a long-lived space-based observatory. It was the flagship mission of NASA's Great Observatories program. The HST program began as an astronomical dream in the 1940s. During the 1970s and 1980s, the HST was finally designed and built becoming operational in the 1990s. The HST was deployed into a low-Earth orbit on April 25, 1990 from the cargo bay of the Space Shuttle Discovery (STS-31). The design of the HST took into consideration its length of service and the necessity of repairs and equipment replacement by making the body modular. In doing so, subsequent shuttle missions could recover the HST, replace faulty or obsolete parts and be re-released. Marshall Space Flight Center's (MSFC's) Neutral Buoyancy Simulator (NBS) served as the test center for shuttle astronauts training for Hubble related missions. Shown is astronaut Anna Fisher suiting up for training on a mockup of a modular section of the HST for an axial scientific instrument change out.

  13. Astronaut Anna Fisher Suiting Up For NBS Training

    Science.gov (United States)

    1980-01-01

    The Hubble Space Telescope (HST) is a cooperative program of the European Space Agency (ESA) and the National Aeronautical and Space Administration (NASA) to operate a long-lived space-based observatory. It was the flagship mission of NASA's Great Observatories program. The HST program began as an astronomical dream in the 1940s. During the 1970s and 1980s, the HST was finally designed and built becoming operational in the 1990s. The HST was deployed into a low-Earth orbit on April 25, 1990 from the cargo bay of the Space Shuttle Discovery (STS-31). The design of the HST took into consideration its length of service and the necessity of repairs and equipment replacement by making the body modular. In doing so, subsequent shuttle missions could recover the HST, replace faulty or obsolete parts and be re-released. Marshall Space Flight Center's (MSFC's) Neutral Buoyancy Simulator (NBS) served as the test center for shuttle astronauts training for Hubble related missions. Shown is astronaut Anna Fisher suiting up for training on a mockup of a modular section of the HST for an axial scientific instrument change out.

  14. Astronaut Anna Fisher Suited Up For NBS Training

    Science.gov (United States)

    1980-01-01

    The Hubble Space Telescope (HST) is a cooperative program of the European Space Agency (ESA) and the National Aeronautical and Space Administration (NASA) to operate a long-lived space-based observatory. It was the flagship mission of NASA's Great Observatories program. The HST program began as an astronomical dream in the 1940s. During the 1970s and 1980s, the HST was finally designed and built becoming operational in the 1990s. The HST was deployed into a low-Earth orbit on April 25, 1990 from the cargo bay of the Space Shuttle Discovery (STS-31). The design of the HST took into consideration its length of service and the necessity of repairs and equipment replacement by making the body modular. In doing so, subsequent shuttle missions could recover the HST, replace faulty or obsolete parts and be re-released. Marshall Space Flight Center's (MSFC's) Neutral Buoyancy Simulator (NBS) served as the test center for shuttle astronauts training for Hubble related missions. Shown is astronaut Anna Fisher suited up for training on a mockup of a modular section of the HST for an axial scientific instrument change out.

  15. Development of an advanced rocket propellant handler's suit

    Science.gov (United States)

    Doerr, DonaldF.

    2001-08-01

    Most launch vehicles and satellites in the US inventory rely upon the use of hypergolic rocket propellants, many of which are toxic to humans. These fuels and oxidizers, such as hydrazine and nitrogen tetroxide have threshold limit values as low as 0.01 PPM. It is essential to provide space workers handling these agents whole body protection as they are universally hazardous not only to the respiratory system, but the skin as well. This paper describes a new method for powering a whole body protective garment to assure the safety of ground servicing crews. A new technology has been developed through the small business innovative research program at the Kennedy Space Center. Currently, liquid air is used in the environmental control unit (ECU) that powers the propellant handlers suit (PHE). However, liquid air exhibits problems with attitude dependence, oxygen enrichment, and difficulty with reliable quantity measurement. The new technology employs the storage of the supply air as a supercritical gas. This method of air storage overcomes all of three problems above while maintaining high density storage at relatively low vessel pressures (protective ensemble marked an advancement in the state-of-the-art in personal protective equipment. Not only was long duration environmental control provided, but it was done without a high pressure vessel. The unit met human performance needs for attitude independence, oxygen stability, and relief of heat stress. This supercritical air (and oxygen) technology is suggested for microgravity applications in life support such as the Extravehicular Mobility Unit.

  16. eXtended CASA Line Analysis Software Suite (XCLASS)

    Science.gov (United States)

    Möller, T.; Endres, C.; Schilke, P.

    2017-02-01

    The eXtended CASA Line Analysis Software Suite (XCLASS) is a toolbox for the Common Astronomy Software Applications package (CASA) containing new functions for modeling interferometric and single dish data. Among the tools is the myXCLASS program which calculates synthetic spectra by solving the radiative transfer equation for an isothermal object in one dimension, whereas the finite source size and dust attenuation are considered as well. Molecular data required by the myXCLASS program are taken from an embedded SQLite3 database containing entries from the Cologne Database for Molecular Spectroscopy (CDMS) and JPL using the Virtual Atomic and Molecular Data Center (VAMDC) portal. Additionally, the toolbox provides an interface for the model optimizer package Modeling and Analysis Generic Interface for eXternal numerical codes (MAGIX), which helps to find the best description of observational data using myXCLASS (or another external model program), that is, finding the parameter set that most closely reproduces the data. http://www.astro.uni-koeln.de/projects/schilke/myXCLASSInterface A copy of the code is available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (http://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/598/A7

  17. California court says disability benefits do not preclude suit.

    Science.gov (United States)

    1998-05-01

    A California appeals court reversed a lower court decision barring a worker from pursuing an HIV discrimination claim against his employer. [Name removed] claims that [name removed] violated California's Fair Employment and Housing Act when it rescinded accommodations that the bank had made earlier for HIV-related medical needs. The accommodations included a compressed work week and one day of telecommuting per week, which [name removed] performed well enough to earn a promotion. With a change in management, the accommodations were canceled, ostensibly to control costs. The lower court ruled that [name removed] was barred from suing his former employer because of statements on his disability insurance application. However, the appeals court ruled that [name removed]'s statements on the form were honest and did not preclude him from future litigation. Myron Quon, an attorney with Lambda Legal Defense and Education Fund in Los Angeles, noted that [name removed]'s deft handling of the questions was vital to the success of the suit. [Name removed] had made comments and notations on the form, rather than just checking the appropriate yes or no boxes, and noted that he could return to work with a reasonable accommodation. Others applying for disability are cautioned to do the same to preserve their legal rights.

  18. A wearable exoskeleton suit for motion assistance to paralysed patients.

    Science.gov (United States)

    Chen, Bing; Zhong, Chun-Hao; Zhao, Xuan; Ma, Hao; Guan, Xiao; Li, Xi; Liang, Feng-Yan; Cheng, Jack Chun Yiu; Qin, Ling; Law, Sheung-Wai; Liao, Wei-Hsin

    2017-10-01

    The number of patients paralysed due to stroke, spinal cord injury, or other related diseases is increasing. In order to improve the physical and mental health of these patients, robotic devices that can help them to regain the mobility to stand and walk are highly desirable. The aim of this study is to develop a wearable exoskeleton suit to help paralysed patients regain the ability to stand up/sit down (STS) and walk. A lower extremity exoskeleton named CUHK-EXO was developed with considerations of ergonomics, user-friendly interface, safety, and comfort. The mechanical structure, human-machine interface, reference trajectories of the exoskeleton hip and knee joints, and control architecture of CUHK-EXO were designed. Clinical trials with a paralysed patient were performed to validate the effectiveness of the whole system design. With the assistance provided by CUHK-EXO, the paralysed patient was able to STS and walk. As designed, the actual joint angles of the exoskeleton well followed the designed reference trajectories, and assistive torques generated from the exoskeleton actuators were able to support the patient's STS and walking motions. The whole system design of CUHK-EXO is effective and can be optimised for clinical application. The exoskeleton can provide proper assistance in enabling paralysed patients to STS and walk.

  19. Freud: a software suite for high-throughput simulation analysis

    Science.gov (United States)

    Harper, Eric; Spellings, Matthew; Anderson, Joshua; Glotzer, Sharon

    Computer simulation is an indispensable tool for the study of a wide variety of systems. As simulations scale to fill petascale and exascale supercomputing clusters, so too does the size of the data produced, as well as the difficulty in analyzing these data. We present Freud, an analysis software suite for efficient analysis of simulation data. Freud makes no assumptions about the system being analyzed, allowing for general analysis methods to be applied to nearly any type of simulation. Freud includes standard analysis methods such as the radial distribution function, as well as new methods including the potential of mean force and torque and local crystal environment analysis. Freud combines a Python interface with fast, parallel C + + analysis routines to run efficiently on laptops, workstations, and supercomputing clusters. Data analysis on clusters reduces data transfer requirements, a prohibitive cost for petascale computing. Used in conjunction with simulation software, Freud allows for smart simulations that adapt to the current state of the system, enabling the study of phenomena such as nucleation and growth, intelligent investigation of phases and phase transitions, and determination of effective pair potentials.

  20. A Coupled Calculation Suite for Atucha II Operational Transients Analysis

    International Nuclear Information System (INIS)

    Mazzantini, O.; Schivo, M.; Cesare, J.D.; Garbero, R.; Rivero, M.; Theler, G.

    2011-01-01

    While more than a decade ago reactor and thermal hydraulic calculations were tedious and often needed a lot of approximations and simplifications that forced the designers to take a very conservative approach, computational resources available nowadays allow engineers to cope with increasingly complex problems in a reasonable time. The use of best-estimate calculations provides tools to justify convenient engineering margins, reduces costs, and maximises economic benefits. In this direction, a suite of coupled best-estimate specific calculation codes was developed to analyse the behaviour of the Atucha II nuclear power plant in Argentina. The developed tool includes three-dimensional spatial neutron kinetics, a channel-level model of the core thermal hydraulics with subcooled boiling correlations, a one-dimensional model of the primary and secondary circuits including pumps, steam generators, heat exchangers, and the turbine with all their associated control loops, and a complete simulation of the reactor control, limitation, and protection system working in closed-loop conditions as a faithful representation of the real power plant. In the present paper, a description of the coupling scheme between the codes involved is given, and some examples of their application to Atucha II are shown

  1. STS-93 Commander Collins suits up before launch

    Science.gov (United States)

    1999-01-01

    In the Operations and Checkout Building, STS-93 Commander Eileen M. Collins gets help donning her launch and entry suit. After Space Shuttle Columbia's July 20 launch attempt was scrubbed at the T-7 second mark in the countdown, the launch was rescheduled for Thursday, July 22, at 12:28 a.m. EDT. The target landing date is July 26, 1999, at 11:24 p.m. EDT. STS-93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The new telescope is 20 to 50 times more sensitive than any previous X- ray telescope and is expected unlock the secrets of supernovae, quasars and black holes. The STS-93 crew numbers five: Commander Collins, Pilot Jeffrey S. Ashby, and Mission Specialists Stephen A. Hawley (Ph.D.), Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  2. STS-93 Commander Collins waves after suiting up before launch

    Science.gov (United States)

    1999-01-01

    During final launch preparations in the Operations and Checkout Building, STS-93 Commander Eileen M. Collins waves after donning her launch and entry suit. After Space Shuttle Columbia's July 20 launch attempt was scrubbed at the T-7 second mark in the countdown, the launch was rescheduled for Thursday, July 22, at 12:28 a.m. EDT. The target landing date is July 26, 1999, at 11:24 p.m. EDT. STS-93 is a five-day mission primarily to release the Chandra X-ray Observatory, which will allow scientists from around the world to study some of the most distant, powerful and dynamic objects in the universe. The new telescope is 20 to 50 times more sensitive than any previous X-ray telescope and is expected unlock the secrets of supernovae, quasars and black holes. The STS-93 crew numbers five: Commander Collins, Pilot Jeffrey S. Ashby, and Mission Specialists Stephen A. Hawley (Ph.D.), Catherine G. Coleman (Ph.D.) and Michel Tognini of France, with the Centre National d'Etudes Spatiales (CNES). Collins is the first woman to serve as commander of a shuttle mission.

  3. The possession law suit, caused by forbidden immissions

    Directory of Open Access Journals (Sweden)

    Popov Danica D.

    2015-01-01

    Full Text Available In the Serbian Law and in most of jurisdictions, there are limits on exercising the right of property. The real estate owner must refrain from activities by which the use of other real estate is being impoded through the immission of execive gasses, vapors, smoke, heat noise, tremors etc. The property ownership whose is affected by immission exceeding the set limits, has the right to request a stop of immisions which exceeded the allowed volume of immissions. In article author describes various kinds of immissions. The general feature of this law suit is that there is only discussion on the facts and not for a legal matters. Subject matter jurisdiction for the resolution of such disputes belongs to the court of general jurisdiction, while the disputes itself is a litigation. The special rule of proceedings of action of disturbance are: provisionality of the protection of possession; urgency in proceedings; initiation of proceedings; limiting of objection; prescribing temporary measures; rendering a ruling in the form of order; appeals which may be filed within a short deadline and which does not have suspensive effect (do not delay the execution of the order; revision is not allowed etc.

  4. The JPEG XT suite of standards: status and future plans

    Science.gov (United States)

    Richter, Thomas; Bruylants, Tim; Schelkens, Peter; Ebrahimi, Touradj

    2015-09-01

    The JPEG standard has known an enormous market adoption. Daily, billions of pictures are created, stored and exchanged in this format. The JPEG committee acknowledges this success and spends continued efforts in maintaining and expanding the standard specifications. JPEG XT is a standardization effort targeting the extension of the JPEG features by enabling support for high dynamic range imaging, lossless and near-lossless coding, and alpha channel coding, while also guaranteeing backward and forward compatibility with the JPEG legacy format. This paper gives an overview of the current status of the JPEG XT standards suite. It discusses the JPEG legacy specification, and details how higher dynamic range support is facilitated both for integer and floating-point color representations. The paper shows how JPEG XT's support for lossless and near-lossless coding of low and high dynamic range images is achieved in combination with backward compatibility to JPEG legacy. In addition, the extensible boxed-based JPEG XT file format on which all following and future extensions of JPEG will be based is introduced. This paper also details how the lossy and lossless representations of alpha channels are supported to allow coding transparency information and arbitrarily shaped images. Finally, we conclude by giving prospects on upcoming JPEG standardization initiative JPEG Privacy & Security, and a number of other possible extensions in JPEG XT.

  5. Improvements to the APBS biomolecular solvation software suite.

    Science.gov (United States)

    Jurrus, Elizabeth; Engel, Dave; Star, Keith; Monson, Kyle; Brandi, Juan; Felberg, Lisa E; Brookes, David H; Wilson, Leighton; Chen, Jiahui; Liles, Karina; Chun, Minju; Li, Peter; Gohara, David W; Dolinsky, Todd; Konecny, Robert; Koes, David R; Nielsen, Jens Erik; Head-Gordon, Teresa; Geng, Weihua; Krasny, Robert; Wei, Guo-Wei; Holst, Michael J; McCammon, J Andrew; Baker, Nathan A

    2018-01-01

    The Adaptive Poisson-Boltzmann Solver (APBS) software was developed to solve the equations of continuum electrostatics for large biomolecular assemblages that have provided impact in the study of a broad range of chemical, biological, and biomedical applications. APBS addresses the three key technology challenges for understanding solvation and electrostatics in biomedical applications: accurate and efficient models for biomolecular solvation and electrostatics, robust and scalable software for applying those theories to biomolecular systems, and mechanisms for sharing and analyzing biomolecular electrostatics data in the scientific community. To address new research applications and advancing computational capabilities, we have continually updated APBS and its suite of accompanying software since its release in 2001. In this article, we discuss the models and capabilities that have recently been implemented within the APBS software package including a Poisson-Boltzmann analytical and a semi-analytical solver, an optimized boundary element solver, a geometry-based geometric flow solvation model, a graph theory-based algorithm for determining pK a values, and an improved web-based visualization tool for viewing electrostatics. © 2017 The Protein Society.

  6. Profiling cancer

    DEFF Research Database (Denmark)

    Ciro, Marco; Bracken, Adrian P; Helin, Kristian

    2003-01-01

    In the past couple of years, several very exciting studies have demonstrated the enormous power of gene-expression profiling for cancer classification and prediction of patient survival. In addition to promising a more accurate classification of cancer and therefore better treatment of patients......, gene-expression profiling can result in the identification of novel potential targets for cancer therapy and a better understanding of the molecular mechanisms leading to cancer....

  7. VOLCWORKS: A suite for optimization of hazards mapping

    Science.gov (United States)

    Delgado Granados, H.; Ramírez Guzmán, R.; Villareal Benítez, J. L.; García Sánchez, T.

    2012-04-01

    Making hazards maps is a process linking basic science, applied science and engineering for the benefit of the society. The methodologies for hazards maps' construction have evolved enormously together with the tools that allow the forecasting of the behavior of the materials produced by different eruptive processes. However, in spite of the development of tools and evolution of methodologies, the utility of hazards maps has not changed: prevention and mitigation of volcanic disasters. Integration of different tools for simulation of different processes for a single volcano is a challenge to be solved using software tools including processing, simulation and visualization techniques, and data structures in order to build up a suit that helps in the construction process starting from the integration of the geological data, simulations and simplification of the output to design a hazards/scenario map. Scientific visualization is a powerful tool to explore and gain insight into complex data from instruments and simulations. The workflow from data collection, quality control and preparation for simulations, to achieve visual and appropriate presentation is a process that is usually disconnected, using in most of the cases different applications for each of the needed processes, because it requires many tools that are not built for the solution of a specific problem, or were developed by research groups to solve particular tasks, but disconnected. In volcanology, due to its complexity, groups typically examine only one aspect of the phenomenon: ash dispersal, laharic flows, pyroclastic flows, lava flows, and ballistic projectile ejection, among others. However, when studying the hazards associated to the activity of a volcano, it is important to analyze all the processes comprehensively, especially for communication of results to the end users: decision makers and planners. In order to solve this problem and connect different parts of a workflow we are developing the

  8. A suite of global, cross-scale topographic variables for environmental and biodiversity modeling

    Science.gov (United States)

    Amatulli, Giuseppe; Domisch, Sami; Tuanmu, Mao-Ning; Parmentier, Benoit; Ranipeta, Ajay; Malczyk, Jeremy; Jetz, Walter

    2018-03-01

    Topographic variation underpins a myriad of patterns and processes in hydrology, climatology, geography and ecology and is key to understanding the variation of life on the planet. A fully standardized and global multivariate product of different terrain features has the potential to support many large-scale research applications, however to date, such datasets are unavailable. Here we used the digital elevation model products of global 250 m GMTED2010 and near-global 90 m SRTM4.1dev to derive a suite of topographic variables: elevation, slope, aspect, eastness, northness, roughness, terrain roughness index, topographic position index, vector ruggedness measure, profile/tangential curvature, first/second order partial derivative, and 10 geomorphological landform classes. We aggregated each variable to 1, 5, 10, 50 and 100 km spatial grains using several aggregation approaches. While a cross-correlation underlines the high similarity of many variables, a more detailed view in four mountain regions reveals local differences, as well as scale variations in the aggregated variables at different spatial grains. All newly-developed variables are available for download at Data Citation 1 and for download and visualization at http://www.earthenv.org/topography.

  9. Developing defensive aids suite technology on a virtual battlefield

    Science.gov (United States)

    Rapanotti, John L.; DeMontigny-Leboeuf, Annie; Palmarini, Marc; Cantin, Andre

    2002-07-01

    Modern anti-tank missiles and the requirement of rapid deployment are limiting the use of passive armour in protecting land vehicles. Vehicle survivability is becoming more dependent on sensors, computers and countermeasures to detect and avoid threats. The integration of various technologies into a Defensive Aids Suite (DAS) can be designed and analyzed by combining field trials and laboratory data with modeling and simulation. MATLAB is used as a quick prototyping tool to model DAS systems and facilitate transfer to other researchers. The DAS model can be transferred from MATLAB or programmed directly in ModSAF (Modular Semi-Automated Forces), which is used to construct the virtual battlefield. Through scripted input files, a fixed battle approach ensures implementation and analysis meeting the requirements of three different interests. These three communities include the scientists and engineers, military and operations research. This approach ensures the modelling of processes known to be important regardless of the level of information available about the system. A system can be modelled phenomenologically until more information is available. Further processing of the simulation can be used to optimize the vehicle for a specific mission. ModSAF will be used to analyze and plan trials and develop DAS technology for future vehicles. Survivability of a DAS-equipped vehicle can be assessed relative to a basic vehicle without a DAS. In later stages, more complete DAS systems will be analyzed to determine the optimum configuration of the DAS components and the effectiveness of a DAS-equipped vehicle for specific missions. These concepts and approach will be discussed in the paper.

  10. BioWord: A sequence manipulation suite for Microsoft Word

    Directory of Open Access Journals (Sweden)

    Anzaldi Laura J

    2012-06-01

    Full Text Available Abstract Background The ability to manipulate, edit and process DNA and protein sequences has rapidly become a necessary skill for practicing biologists across a wide swath of disciplines. In spite of this, most everyday sequence manipulation tools are distributed across several programs and web servers, sometimes requiring installation and typically involving frequent switching between applications. To address this problem, here we have developed BioWord, a macro-enabled self-installing template for Microsoft Word documents that integrates an extensive suite of DNA and protein sequence manipulation tools. Results BioWord is distributed as a single macro-enabled template that self-installs with a single click. After installation, BioWord will open as a tab in the Office ribbon. Biologists can then easily manipulate DNA and protein sequences using a familiar interface and minimize the need to switch between applications. Beyond simple sequence manipulation, BioWord integrates functionality ranging from dyad search and consensus logos to motif discovery and pair-wise alignment. Written in Visual Basic for Applications (VBA as an open source, object-oriented project, BioWord allows users with varying programming experience to expand and customize the program to better meet their own needs. Conclusions BioWord integrates a powerful set of tools for biological sequence manipulation within a handy, user-friendly tab in a widely used word processing software package. The use of a simple scripting language and an object-oriented scheme facilitates customization by users and provides a very accessible educational platform for introducing students to basic bioinformatics algorithms.

  11. BioWord: A sequence manipulation suite for Microsoft Word

    Science.gov (United States)

    2012-01-01

    Background The ability to manipulate, edit and process DNA and protein sequences has rapidly become a necessary skill for practicing biologists across a wide swath of disciplines. In spite of this, most everyday sequence manipulation tools are distributed across several programs and web servers, sometimes requiring installation and typically involving frequent switching between applications. To address this problem, here we have developed BioWord, a macro-enabled self-installing template for Microsoft Word documents that integrates an extensive suite of DNA and protein sequence manipulation tools. Results BioWord is distributed as a single macro-enabled template that self-installs with a single click. After installation, BioWord will open as a tab in the Office ribbon. Biologists can then easily manipulate DNA and protein sequences using a familiar interface and minimize the need to switch between applications. Beyond simple sequence manipulation, BioWord integrates functionality ranging from dyad search and consensus logos to motif discovery and pair-wise alignment. Written in Visual Basic for Applications (VBA) as an open source, object-oriented project, BioWord allows users with varying programming experience to expand and customize the program to better meet their own needs. Conclusions BioWord integrates a powerful set of tools for biological sequence manipulation within a handy, user-friendly tab in a widely used word processing software package. The use of a simple scripting language and an object-oriented scheme facilitates customization by users and provides a very accessible educational platform for introducing students to basic bioinformatics algorithms. PMID:22676326

  12. Response Surface Modeling Tool Suite, Version 1.x

    Energy Technology Data Exchange (ETDEWEB)

    2016-07-05

    The Response Surface Modeling (RSM) Tool Suite is a collection of three codes used to generate an empirical interpolation function for a collection of drag coefficient calculations computed with Test Particle Monte Carlo (TPMC) simulations. The first code, "Automated RSM", automates the generation of a drag coefficient RSM for a particular object to a single command. "Automated RSM" first creates a Latin Hypercube Sample (LHS) of 1,000 ensemble members to explore the global parameter space. For each ensemble member, a TPMC simulation is performed and the object drag coefficient is computed. In the next step of the "Automated RSM" code, a Gaussian process is used to fit the TPMC simulations. In the final step, Markov Chain Monte Carlo (MCMC) is used to evaluate the non-analytic probability distribution function from the Gaussian process. The second code, "RSM Area", creates a look-up table for the projected area of the object based on input limits on the minimum and maximum allowed pitch and yaw angles and pitch and yaw angle intervals. The projected area from the look-up table is used to compute the ballistic coefficient of the object based on its pitch and yaw angle. An accurate ballistic coefficient is crucial in accurately computing the drag on an object. The third code, "RSM Cd", uses the RSM generated by the "Automated RSM" code and the projected area look-up table generated by the "RSM Area" code to accurately compute the drag coefficient and ballistic coefficient of the object. The user can modify the object velocity, object surface temperature, the translational temperature of the gas, the species concentrations of the gas, and the pitch and yaw angles of the object. Together, these codes allow for the accurate derivation of an object's drag coefficient and ballistic coefficient under any conditions with only knowledge of the object's geometry and mass.

  13. LANDFIRE Remap: A New National Baseline Product Suite

    Science.gov (United States)

    Dockter, D.; Peterson, B.; Picotte, J. J.; Long, J.; Tolk, B.; Callahan, K.; Davidson, A.; Earnhardt, T.

    2017-12-01

    LANDFIRE, also known as the Landscape Fire and Resource Management Planning Tools Program, is a vegetation, fire, and fuel characteristic data creation program managed by both the U.S. Department of Agriculture Forest Service and the U.S. Department of the Interior with involvement from The Nature Conservancy. LANDFIRE represents the first and only complete, nationally consistent collection of over 20 geo-spatial layers (e.g., vegetation type and structure, fuels, fire regimes), databases, and ecological models that can be used across multiple disciplines to support cross-boundary planning, management, and operations across all lands of the United States and insular areas. Since 2004, LANDFIRE has produced comprehensive, consistent, and scientifically based suites of mapped products and associated databases for the United States and affiliated territories. These products depict the nation's major ecosystems and wildlife habitats. Over a decade has passed since the development of the first LANDFIRE base map, and an overhaul of the data products, i.e., a "Remap", is needed to maintain their functionality and relevance. To prepare for Remap production LANDFIRE has invested in a prototyping phase that focused on exploring various input data sources and new modeling and mapping techniques. While still grounded in a solid base consisting of Landsat imagery and high-quality field observations, the prototyping efforts explored different image compositing techniques, the integration of lidar data, modeling approaches as well as other factors that will inform Remap production. Several of these various research efforts are highlighted here and are currently being integrated into an end-to-end data processing flow that will drive the Remap production. The current Remap prototype effort has focused on several study areas throughout CONUS, with additional studies anticipated for Alaska, Hawaii and the territories. The LANDFIRE Remap effort is expected to take three to four years

  14. BioWord: a sequence manipulation suite for Microsoft Word.

    Science.gov (United States)

    Anzaldi, Laura J; Muñoz-Fernández, Daniel; Erill, Ivan

    2012-06-07

    The ability to manipulate, edit and process DNA and protein sequences has rapidly become a necessary skill for practicing biologists across a wide swath of disciplines. In spite of this, most everyday sequence manipulation tools are distributed across several programs and web servers, sometimes requiring installation and typically involving frequent switching between applications. To address this problem, here we have developed BioWord, a macro-enabled self-installing template for Microsoft Word documents that integrates an extensive suite of DNA and protein sequence manipulation tools. BioWord is distributed as a single macro-enabled template that self-installs with a single click. After installation, BioWord will open as a tab in the Office ribbon. Biologists can then easily manipulate DNA and protein sequences using a familiar interface and minimize the need to switch between applications. Beyond simple sequence manipulation, BioWord integrates functionality ranging from dyad search and consensus logos to motif discovery and pair-wise alignment. Written in Visual Basic for Applications (VBA) as an open source, object-oriented project, BioWord allows users with varying programming experience to expand and customize the program to better meet their own needs. BioWord integrates a powerful set of tools for biological sequence manipulation within a handy, user-friendly tab in a widely used word processing software package. The use of a simple scripting language and an object-oriented scheme facilitates customization by users and provides a very accessible educational platform for introducing students to basic bioinformatics algorithms.

  15. Interaction of Space Suits with Windblown Soil: Preliminary Mars Wind Tunnel Results

    Science.gov (United States)

    Marshall, J.; Bratton, C.; Kosmo, J.; Trevino, R.

    1999-09-01

    Experiments in the Mars Wind Tunnel at NASA Ames Research Center show that under Mars conditions, spacesuit materials are highly susceptible to dust contamination when exposed to windblown soil. This effect was suspected from knowledge of the interaction of electrostatically adhesive dust with solid surfaces in general. However, it is important to evaluate the respective roles of materials, meteorological and radiation effects, and the character of the soil. The tunnel permits evaluation of dust contamination and sand abrasion of space suits by simulating both pressure and wind conditions on Mars. The long-term function of space suits on Mars will be primarily threatened by dust contamination. Lunar EVA activities caused heavy contamination of space suits, but the problem was never seriously manifest because of the brief utilization of the suits, and the suits were never reused. Electrostatically adhering dust grains have various detrimental effects: (1) penetration and subsequent wear of suit fabrics, (2) viewing obscuration through visors and scratching/pitting of visor surfaces, (3) penetration, wear, and subsequent seizing-up of mechanical suit joints, (4) changes in albedo and therefore of radiation properties of external heat-exchanger systems, (5) changes in electrical conductivity of suit surfaces which may affect tribocharging of suits and create spurious discharge effects detrimental to suit electronics/radio systems. Additional information is contained in the original.

  16. Immunogenicity and protective efficacy of Brucella abortus recombinant protein cocktail (rOmp19+rP39) against B. abortus 544 and B. melitensis 16M infection in murine model.

    Science.gov (United States)

    Tadepalli, Ganesh; Singh, Amit Kumar; Balakrishna, Konduru; Murali, Harishchandra Sripathy; Batra, Harsh Vardhan

    2016-03-01

    In this study, the immunogenicity and protective efficacy of recombinant proteins Omp19 (rO) and P39 (rP) from Brucella abortus were evaluated individually and compared with the cocktail protein (rO+rP) against B. abortus 544 and Brucella melitensis 16M infection in BALB/c mouse model. Intra-peritoneal (I.P.) immunization with rO+rP cocktail developed substantially higher antibody titers predominant with Th1 mediated isotypes (IgG2a/2b). Western blot analysis using anti-rO+rP antibodies showed specific reactivity with native Omp19 (19 kDa) and P39 (39 kDa) among whole cell proteins of B. abortus and B. melitensis. Splenocytes extracted from rO+rP immunized mice induced significantly (Pabortus 544 (72.27%) and B. melitensis 16M (68.57%). On the other hand, individual anti-rO and anti-rP polysera resulted in relatively lesser protection against the pathogens (64.79%, 54.45% and 47.13%, 45.11%, respectively). Immunized group of mice when I.P. challenged with 5 × 10(4) CFU of B. abortus 544 and B. melitensis 16M were found significantly (PBrucella vaccine. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. Comparison of potential protection conferred by three immunization strategies (protein/protein, DNA/DNA, and DNA/protein) against Brucella infection using Omp2b in BALB/c Mice.

    Science.gov (United States)

    Golshani, Maryam; Rafati, Sima; Nejati-Moheimani, Mehdi; Ghasemian, Melina; Bouzari, Saeid

    2016-12-25

    In the present study, immunogenicity and protective efficacy of the Brucella outer membrane protein 2b (Omp2b) was evaluated in BALB/c mice using Protein/Protein, DNA/DNA and DNA/Protein vaccine strategies. Immunization of mice with three vaccine regimens elicited a strong specific IgG response (higher IgG2a titers over IgG1 titers) and provided Th1-oriented immune response. Vaccination of BALB/c mice with the DNA/Pro regimen induced higher levels of IFN-γ/IL-2 and conferred more protection levels against B. melitenisis and B. abortus challenge than did the protein or DNA alone. In conclusion, Omp2b is able to stimulate specific immune responses and to confer cross protection against B. melitensis and B. abortus infection. Therefore, it could be introduced as a new potential candidate for the development of a subunit vaccine against Brucella infection. Copyright © 2016 Elsevier B.V. All rights reserved.

  18. Assessment of Protective Gloves for Use with Airfed Suits.

    Science.gov (United States)

    Millard, Claire E; Vaughan, Nicholas P

    2015-10-01

    Gloves are often needed for hand protection at work, but they can impair manual dexterity, especially if they are multilayered or ill-fitting. This article describes two studies of gloves to be worn with airfed suits (AFS) for nuclear decommissioning or containment level 4 (CL4) microbiological work. Both sets of workers wear multiple layers of gloves for protection and to accommodate decontamination procedures. Nuclear workers are also often required to wear cut-resistant gloves as an extra layer of protection. A total of 15 subjects volunteered to take part in manual dexterity testing of the different gloving systems. The subjects' hands were measured to ensure that the appropriate sized gloves were used. The gloves were tested with the subjects wearing the complete clothing ensembles appropriate to the work, using a combination of standard dexterity tests: the nine-hole peg test; a pin test adapted from the European Standard for protective gloves, the Purdue Pegboard test, and the Minnesota turning test. Specialized tests such as a hand tool test were used to test nuclear gloves, and laboratory-type manipulation tasks were used to test CL4 gloves. Subjective assessments of temperature sensation and skin wettedness were made before and after the dexterity tests of the nuclear gloves only. During all assessments, we made observations and questioned the subjects about ergonomic issues related to the clothing ensembles. Overall, the results show that the greater the thickness of the gloves and the number of layers the more the levels of manual dexterity performance are degraded. The nuclear cut-resistant gloves with the worst level of dexterity were stiff and inflexible and the subjects experienced problems picking up small items and bending their hands. The work also highlighted other factors that affect manual dexterity performance, including proper sizing, interactions with the other garments worn at the time, and the work equipment in use. In conclusion, when

  19. Oracle E-Business Suite Financials R12 A Functionality Guide

    CERN Document Server

    Iyer, Mohan

    2012-01-01

    This is a step-by-step functional guide to get you started easily with Oracle EBS Financials. If you are an Oracle E-Business Suite Financial consultant or an administrator looking to get a quick review on the capabilities of Oracle E-Business Suite and improve the use of the systems functionality, then this is the best guide for you. This book assumes that you have a fundamental knowledge of EBS Suite.

  20. Gamma-ray spectrometry of granitic suites of the Paranaguá Terrane, Southern Brazil

    Science.gov (United States)

    Weihermann, Jessica Derkacz; Ferreira, Francisco José Fonseca; Cury, Leonardo Fadel; da Silveira, Claudinei Taborda

    2016-09-01

    The Paranaguá Terrane, located in the coastal portion of the states of Santa Catarina, Paraná and São Paulo in Southern Brazil is a crustal segment constituted mainly by an igneous complex, with a variety of granitic rocks inserted into the Serra do Mar ridge. The average altitude is approximately 1200 m above sea level, with peaks of up to 1800 m. Due to the difficulty of accessing the area, a shortage of outcrops and the thick weathering mantle, this terrane is understudied. This research aims to evaluate the gamma-ray spectrometry data of the granitic suites of the Paranaguá Terrane, in correspondence with the geological, petrographical, lithogeochemical, relief and mass movement information available in the literature. Aerogeophysical data were acquired along north-south lines spaced at 500 m, with a mean terrain clearance of 100 m. These data cover potassium (K, %), equivalent in thorium (eTh, ppm) and equivalent in uranium (eU, ppm). After performing a critical analysis of the data, basic (K, eU, eTh) and ternary (R-K/G-eTh/B-eU) maps were generated and then superimposed on the digital elevation model (DEM). The investigation of the radionuclide mobility across the relief and weathering mantle consisted of an analysis of the schematic profiles of elevation related with each radionuclide; a comparison of the K, eU and eTh maps with their 3D correspondents; and the study of mass movements registered in the region. A statistical comparison of lithogeochemical (K, U, Th) and geophysical (K, eU, eTh) data showed consistency in all the granitic suites studied (Morro Inglês, Rio do Poço and Canavieiras-Estrela). Through gamma-ray spectrometry, it was possible to establish relationships between scars (from mass movements) and the gamma-ray responses as well as the radionuclide mobility and the relief and to map the granitic bodies.

  1. A Parametric Model of Shoulder Articulation for Virtual Assessment of Space Suit Fit

    Science.gov (United States)

    Kim, K. Han; Young, Karen S.; Bernal, Yaritza; Boppana, Abhishektha; Vu, Linh Q.; Benson, Elizabeth A.; Jarvis, Sarah; Rajulu, Sudhakar L.

    2016-01-01

    Suboptimal suit fit is a known risk factor for crewmember shoulder injury. Suit fit assessment is however prohibitively time consuming and cannot be generalized across wide variations of body shapes and poses. In this work, we have developed a new design tool based on the statistical analysis of body shape scans. This tool is aimed at predicting the skin deformation and shape variations for any body size and shoulder pose for a target population. This new process, when incorporated with CAD software, will enable virtual suit fit assessments, predictively quantifying the contact volume, and clearance between the suit and body surface at reduced time and cost.

  2. A Method for and Issues Associated with the Determination of Space Suit Joint Requirements

    Science.gov (United States)

    Matty, Jennifer E.; Aitchison, Lindsay

    2009-01-01

    In the design of a new space suit it is necessary to have requirements that define what mobility space suit joints should be capable of achieving in both a system and at the component level. NASA elected to divide mobility into its constituent parts-range of motion (ROM) and torque- in an effort to develop clean design requirements that limit subject performance bias and are easily verified. Unfortunately, the measurement of mobility can be difficult to obtain. Current technologies, such as the Vicon motion capture system, allow for the relatively easy benchmarking of range of motion (ROM) for a wide array of space suit systems. The ROM evaluations require subjects in the suit to accurately evaluate the ranges humans can achieve in the suit. However, when it comes to torque, there are significant challenges for both benchmarking current performance and writing requirements for future suits. This is reflected in the fact that torque definitions have been applied to very few types of space suits and with limited success in defining all the joints accurately. This paper discussed the advantages and disadvantages to historical joint torque evaluation methods, describes more recent efforts directed at benchmarking joint torques of prototype space suits, and provides an outline for how NASA intends to address joint torque in design requirements for the Constellation Space Suit System (CSSS).

  3. Characterization of the Radiation Shielding Properties of US and Russian EVA Suits

    International Nuclear Information System (INIS)

    Benton, E.R.; Benton, E.V.; Frank, A.L.

    2001-01-01

    Reported herein are results from the Eril Research, Inc. (ERI) participation in the NASA Johnson Space Center sponsored study characterizing the radiation shielding properties of the two types of space suit that astronauts are wearing during the EVA on-orbit assembly of the International Space Station (ISS). Measurements using passive detectors were carried out to assess the shielding properties of the USEMU Suit and the Russian Orlan-M suit during irradiations of the suits and a tissue equivalent phantom to monoenergetic proton and electron beams at the Loma Linda University Medical Center (LLUMC). During irradiations of 6 MeV electrons and 60 MeV protons, absorbed dose as a function of depth was measured using TLDs exposed behind swatches of the two suit materials and inside the two EVA helmets. Considerable reduction in electron dose was measured behind all suit materials in exposures to 6MeV electrons. Slowing of the proton beam in the suit materials led to an increase in dose measured in exposures to 60 MeV protons. During 232 MeV proton irradiations, measurements were made with TLDs and CR-39 PNTDs at five organ locations inside a tissue equivalent phantom, exposed both with and without the two EVA suits. The EVA helmets produce a 13 to 27 percent reduction in total dose and a 0 to 25 percent reduction in dose equivalent when compared to measurements made in the phantom head alone. Differences in dose and dose equivalent between the suit and non-suit irradiations for the lower portions of the two EVA suits tended to be smaller. Proton-induced target fragmentation was found to be a significant source of increased dose equivalent, especially within the two EVA helmets, and average quality factor inside the EMU and Orlan-M helmets was 2 to 14 percent greater than that measured in the bare phantom head

  4. Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 1. Biosafety Level 4 Suit Laboratory Suite Entry and Exit Procedures.

    Science.gov (United States)

    Janosko, Krisztina; Holbrook, Michael R; Adams, Ricky; Barr, Jason; Bollinger, Laura; Newton, Je T'aime; Ntiforo, Corrie; Coe, Linda; Wada, Jiro; Pusl, Daniela; Jahrling, Peter B; Kuhn, Jens H; Lackemeyer, Matthew G

    2016-10-03

    Biosafety level 4 (BSL-4) suit laboratories are specifically designed to study high-consequence pathogens for which neither infection prophylaxes nor treatment options exist. The hallmarks of these laboratories are: custom-designed airtight doors, dedicated supply and exhaust airflow systems, a negative-pressure environment, and mandatory use of positive-pressure ("space") suits. The risk for laboratory specialists working with highly pathogenic agents is minimized through rigorous training and adherence to stringent safety protocols and standard operating procedures. Researchers perform the majority of their work in BSL-2 laboratories and switch to BSL-4 suit laboratories when work with a high-consequence pathogen is required. Collaborators and scientists considering BSL-4 projects should be aware of the challenges associated with BSL-4 research both in terms of experimental technical limitations in BSL-4 laboratory space and the increased duration of such experiments. Tasks such as entering and exiting the BSL-4 suit laboratories are considerably more complex and time-consuming compared to BSL-2 and BSL-3 laboratories. The focus of this particular article is to address basic biosafety concerns and describe the entrance and exit procedures for the BSL-4 laboratory at the NIH/NIAID Integrated Research Facility at Fort Detrick. Such procedures include checking external systems that support the BSL-4 laboratory, and inspecting and donning positive-pressure suits, entering the laboratory, moving through air pressure-resistant doors, and connecting to air-supply hoses. We will also discuss moving within and exiting the BSL-4 suit laboratories, including using the chemical shower and removing and storing positive-pressure suits.

  5. The Role of KREEP in the Production of Mg-Suite Magmas and Its Influence on the Extent of Mg-Suite Magmatism in the Lunar Crust

    Science.gov (United States)

    Elardo, S. M.; Shearer, C. K.; McCubbin, F. M.

    2017-01-01

    The lunar magnesian-suite, or Mg-suite, is a series of ancient plutonic rocks from the lunar crust. They have received a considerable amount of attention from lunar scientists since their discovery for three primary reasons: 1) their ages and geochemistry indicate they represent pristine magmatic samples that crystallized very soon after the formation of the Moon; 2) their ages often overlap with ages of the ferroan anorthosite (FAN) crust; and 3) planetary-scale processes are needed in formation models to account for their unique geochemical features. Taken as a whole, the Mg-suite samples, as magmatic cumulate rocks, approximate a fractional crystallization sequence in the low-pressure forsterite-anorthite-silica system, and thus these samples are generally thought to be derived from layered mafic intrusions which crystallized very slowly from magmas that intruded the anorthositic crust. However, no direct linkages have been established between different Mg-suite samples based either on field relationships or geochemistry.The model for the origin of the Mg-suite, which best fits the limited available data, is one where Mg-suite magmas form from melting of a hybrid cumulate package consisting of deep mantle dunite, crustal anorthosite, and KREEP (potassium-rare earth elements-phosphorus) at the base of the crust under the Procellarum KREEP Terrane (PKT). In this model, these three LMO (Lunar Magma Ocean) cumulate components are brought into close proximity by the cumulate overturn process. Deep mantle dunitic cumulates with an Mg number of approximately 90 rise to the base of the anorthositic crust due to their buoyancy relative to colder, more dense Fe- and Ti-rich cumulates. This hybridized source rock melts to form Mg-suite magmas, saturated in Mg-rich olivine and anorthitic plagioclase, that have a substantial KREEP component.

  6. Yersinia enterocolitica-Specific Infection by Bacteriophages TG1 and ϕR1-RT Is Dependent on Temperature-Regulated Expression of the Phage Host Receptor OmpF.

    Science.gov (United States)

    Leon-Velarde, Carlos G; Happonen, Lotta; Pajunen, Maria; Leskinen, Katarzyna; Kropinski, Andrew M; Mattinen, Laura; Rajtor, Monika; Zur, Joanna; Smith, Darren; Chen, Shu; Nawaz, Ayesha; Johnson, Roger P; Odumeru, Joseph A; Griffiths, Mansel W; Skurnik, Mikael

    2016-09-01

    Bacteriophages present huge potential both as a resource for developing novel tools for bacterial diagnostics and for use in phage therapy. This potential is also valid for bacteriophages specific for Yersinia enterocolitica To increase our knowledge of Y. enterocolitica-specific phages, we characterized two novel yersiniophages. The genomes of the bacteriophages vB_YenM_TG1 (TG1) and vB_YenM_ϕR1-RT (ϕR1-RT), isolated from pig manure in Canada and from sewage in Finland, consist of linear double-stranded DNA of 162,101 and 168,809 bp, respectively. Their genomes comprise 262 putative coding sequences and 4 tRNA genes and share 91% overall nucleotide identity. Based on phylogenetic analyses of their whole-genome sequences and large terminase subunit protein sequences, a genus named Tg1virus within the family Myoviridae is proposed, with TG1 and ϕR1-RT (R1RT in the ICTV database) as member species. These bacteriophages exhibit a host range restricted to Y. enterocolitica and display lytic activity against the epidemiologically significant serotypes O:3, O:5,27, and O:9 at and below 25°C. Adsorption analyses of lipopolysaccharide (LPS) and OmpF mutants demonstrate that these phages use both the LPS inner core heptosyl residues and the outer membrane protein OmpF as phage receptors. Based on RNA sequencing and quantitative proteomics, we also demonstrate that temperature-dependent infection is due to strong repression of OmpF at 37°C. In addition, ϕR1-RT was shown to be able to enter into a pseudolysogenic state. Together, this work provides further insight into phage-host cell interactions by highlighting the importance of understanding underlying factors which may affect the abundance of phage host receptors on the cell surface. Only a small number of bacteriophages infecting Y. enterocolitica, the predominant causative agent of yersiniosis, have been previously described. Here, two newly isolated Y. enterocolitica phages were studied in detail, with the aim of

  7. High Performance Electrical Modeling and Simulation Verification Test Suite - Tier I; TOPICAL

    International Nuclear Information System (INIS)

    SCHELLS, REGINA L.; BOGDAN, CAROLYN W.; WIX, STEVEN D.

    2001-01-01

    This document describes the High Performance Electrical Modeling and Simulation (HPEMS) Global Verification Test Suite (VERTS). The VERTS is a regression test suite used for verification of the electrical circuit simulation codes currently being developed by the HPEMS code development team. This document contains descriptions of the Tier I test cases

  8. Modular Algorithm Testbed Suite (MATS): A Software Framework for Automatic Target Recognition

    Science.gov (United States)

    2017-01-01

    NAVAL SURFACE WARFARE CENTER PANAMA CITY DIVISION PANAMA CITY, FL 32407-7001 TECHNICAL REPORT NSWC PCD TR-2017-004 MODULAR ...31-01-2017 Technical Modular Algorithm Testbed Suite (MATS): A Software Framework for Automatic Target Recognition DR...flexible platform to facilitate the development and testing of ATR algorithms. To that end, NSWC PCD has created the Modular Algorithm Testbed Suite

  9. Surgical Space Suits Increase Particle and Microbiological Emission Rates in a Simulated Surgical Environment.

    Science.gov (United States)

    Vijaysegaran, Praveen; Knibbs, Luke D; Morawska, Lidia; Crawford, Ross W

    2018-05-01

    The role of space suits in the prevention of orthopedic prosthetic joint infection remains unclear. Recent evidence suggests that space suits may in fact contribute to increased infection rates, with bioaerosol emissions from space suits identified as a potential cause. This study aimed to compare the particle and microbiological emission rates (PER and MER) of space suits and standard surgical clothing. A comparison of emission rates between space suits and standard surgical clothing was performed in a simulated surgical environment during 5 separate experiments. Particle counts were analyzed with 2 separate particle counters capable of detecting particles between 0.1 and 20 μm. An Andersen impactor was used to sample bacteria, with culture counts performed at 24 and 48 hours. Four experiments consistently showed statistically significant increases in both PER and MER when space suits are used compared with standard surgical clothing. One experiment showed inconsistent results, with a trend toward increases in both PER and MER when space suits are used compared with standard surgical clothing. Space suits cause increased PER and MER compared with standard surgical clothing. This finding provides mechanistic evidence to support the increased prosthetic joint infection rates observed in clinical studies. Copyright © 2017 Elsevier Inc. All rights reserved.

  10. Virtual reality simulation training in a high-fidelity procedure suite

    DEFF Research Database (Denmark)

    Lönn, Lars; Edmond, John J; Marco, Jean

    2012-01-01

    To assess the face and content validity of a novel, full physics, full procedural, virtual reality simulation housed in a hybrid procedure suite.......To assess the face and content validity of a novel, full physics, full procedural, virtual reality simulation housed in a hybrid procedure suite....

  11. A suite of models to support the quantitative assessment of spread in pest risk analysis

    NARCIS (Netherlands)

    Robinet, C.; Kehlenbeck, H.; Werf, van der W.

    2012-01-01

    In the frame of the EU project PRATIQUE (KBBE-2007-212459 Enhancements of pest risk analysis techniques) a suite of models was developed to support the quantitative assessment of spread in pest risk analysis. This dataset contains the model codes (R language) for the four models in the suite. Three

  12. Telemetry Standards, IRIG Standard 106-17, Chapter 22, Network Based Protocol Suite

    Science.gov (United States)

    2017-07-01

    requirements. 22.2 Network Access Layer 22.2.1 Physical Layer Connectors and cable media should meet the electrical or optical properties required by the...Telemetry Standards, IRIG Standard 106-17 Chapter 22, July 2017 i CHAPTER 22 Network -Based Protocol Suite Acronyms...iii Chapter 22. Network -Based Protocol Suite

  13. Fluid replacement advice during work in fully encapsulated impermeable chemical protective suits

    NARCIS (Netherlands)

    Rubenstein, C.D.; Hartog, E.A. den; Deaton, A.S.; Bogerd, C.P.; Kant, S. de

    2017-01-01

    A major concern for responders to hazardous materials (HazMat) incidents is the heat strain that is caused by fully encapsulated impermeable chemical protective suits. In a research project, funded by the US Department of Defense, the thermal strain experienced when wearing these suits was studied.

  14. DEMON/ANGEL - A SUITE OF PROGRAMS TO CARRY OUT DENSITY MODIFICATION

    NARCIS (Netherlands)

    VELLIEUX, FMDAP; HUNT, JF; ROY, S; READ, RJ

    1995-01-01

    The DEMON/ANGEL suite of computer programs has been developed to carry out density modification by non-crystallographic symmetry-averaging, solvent-flattening and histogram-mapping techniques. This suite consists of programs that allow molecular envelopes to be defined and modified,

  15. Statistical Evaluation of Causal Factors Associated with Astronaut Shoulder Injury in Space Suits.

    Science.gov (United States)

    Anderson, Allison P; Newman, Dava J; Welsch, Roy E

    2015-07-01

    Shoulder injuries due to working inside the space suit are some of the most serious and debilitating injuries astronauts encounter. Space suit injuries occur primarily in the Neutral Buoyancy Laboratory (NBL) underwater training facility due to accumulated musculoskeletal stress. We quantitatively explored the underlying causal mechanisms of injury. Logistic regression was used to identify relevant space suit components, training environment variables, and anthropometric dimensions related to an increased propensity for space-suited injury. Two groups of subjects were analyzed: those whose reported shoulder incident is attributable to the NBL or working in the space suit, and those whose shoulder incidence began in active duty, meaning working in the suit could be a contributing factor. For both groups, percent of training performed in the space suit planar hard upper torso (HUT) was the most important predictor variable for injury. Frequency of training and recovery between training were also significant metrics. The most relevant anthropometric dimensions were bideltoid breadth, expanded chest depth, and shoulder circumference. Finally, record of previous injury was found to be a relevant predictor for subsequent injury. The first statistical model correctly identifies 39% of injured subjects, while the second model correctly identifies 68% of injured subjects. A review of the literature suggests this is the first work to quantitatively evaluate the hypothesized causal mechanisms of all space-suited shoulder injuries. Although limited in predictive capability, each of the identified variables can be monitored and modified operationally to reduce future impacts on an astronaut's health.

  16. Study of the suit inflation effect on crew safety during landing using a full-pressure IVA suit for new-generation reentry space vehicles

    Science.gov (United States)

    Wataru, Suzuki

    Recently, manned space capsules have been recognized as beneficial and reasonable human space vehicles again. The Dragon capsule already achieved several significant successes. The Orion capsule is going to be sent to a high-apogee orbit without crews for experimental purposes in September 2014. For such human-rated space capsules, the study of acceleration impacts against the human body during splashdown is essential to ensure the safety of crews. Moreover, it is also known that wearing a full pressure rescue suit significantly increases safety of a crew, compared to wearing a partial pressure suit. This is mainly because it enables the use of a personal life support system independently in addition to that which installed in the space vehicle. However, it is unclear how the inflation of the full pressure suit due to pressurization affects the crew safety during splashdown, especially in the case of the new generation manned space vehicles. Therefore, the purpose of this work is to investigate the effect of the suit inflation on crew safety against acceleration impact during splashdown. For this objective, the displacements of the safety harness in relation with the suit, a human surrogate, and the crew seats during pressurizing the suit in order to determine if the safety and survivability of a crew can be improved by wearing a full pressure suit. For these tests, the DL/H-1 full pressure IVA suit, developed by Pablo de Leon and Gary L. Harris, will be used. These tests use image analysis techniques to determine the displacements. It is expected, as a result of these tests, that wearing a full pressure suit will help to mitigate the impacts and will increase the safety and survivability of a crew during landing since it works as a buffer to mitigate impact forces during splashdown. This work also proposes a future plan for sled test experiments using a sled facility such as the one in use by the Civil Aerospace Medical Institute (CAMI) for experimental validation

  17. The antigravity suit in neurosurgery. Cardiovascular responses in seated neurosurgical patients.

    Science.gov (United States)

    Brodrick, P M; Ingram, G S

    1988-09-01

    The haemodynamic responses associated with inflation of the antigravity suit (G suit, aviation type) to 8.0 kPa were studied in a series of 40 patients who underwent neurosurgical operations in the sitting position. The study showed statistically significant increases in systolic arterial pressure (p less than 0.005) and mean central venous pressure (p less than 0.001) with inflation of the suit. The systolic arterial and mean central venous pressures remained significantly elevated immediately before deflation of the suit at the end of the operation (p less than 0.001 and p less than 0.005 respectively). The addition of 0.8-1.0 kPa positive end expiratory pressure during suit inflation was also investigated. A further increase in central venous pressure occurred but this did not achieve statistical significance.

  18. A transitional alkalic dolerite dike suite of Mesozoic age in Southeastern New England

    Science.gov (United States)

    Hermes, O. Don; Rao, J. M.; Dickenson, M. P.; Pierce, T. A.

    1984-12-01

    Dike rocks from the New England platform of Rhode Island and adjacent Massachusetts consist of premetamorphic and post-metamorphic suites. The older group includes metamorphosed dolerite, minette, and schistose dioritic rocks. Post-metamorphic dikes consist of dolerite and sparse monchiquite. The post-metamorphic dolerites are of comparable age to the Eastern North American dolerite suite associated with the Mesozoic basins along the eastern seaboard of North America. However, the southeastern New England dolerites exhibit mineralogy and chemistry more typical of a transitional alkalic suite compared to the more subalkalic tholeiitic dolerites of the Eastern North American suite. Both suites are compatible with a rift tectonic setting, but the more alkalic dolerites may represent a deeper source of small volume melts compared to the Eastern North American dolerites. These more alkaline melts may have concentrated at local centers, or they may be typical of flank dolerites as opposed to the less alkalic varieties that occur within the central axial rift.

  19. Space Suit Performance: Methods for Changing the Quality of Quantitative Data

    Science.gov (United States)

    Cowley, Matthew; Benson, Elizabeth; Rajulu, Sudhakar

    2014-01-01

    NASA is currently designing a new space suit capable of working in deep space and on Mars. Designing a suit is very difficult and often requires trade-offs between performance, cost, mass, and system complexity. To verify that new suits will enable astronauts to perform to their maximum capacity, prototype suits must be built and tested with human subjects. However, engineers and flight surgeons often have difficulty understanding and applying traditional representations of human data without training. To overcome these challenges, NASA is developing modern simulation and analysis techniques that focus on 3D visualization. Early understanding of actual performance early on in the design cycle is extremely advantageous to increase performance capabilities, reduce the risk of injury, and reduce costs. The primary objective of this project was to test modern simulation and analysis techniques for evaluating the performance of a human operating in extra-vehicular space suits.

  20. Integrated Suit Test 1 - A Study to Evaluate Effects of Suit Weight, Pressure, and Kinematics on Human Performance during Lunar Ambulation

    Science.gov (United States)

    Gernhardt, Michael L.; Norcross, Jason; Vos, Jessica R.

    2008-01-01

    In an effort to design the next generation Lunar suit, NASA has initiated a series of tests aimed at understanding the human physiological and biomechanical affects of space suits under a variety of conditions. The first of these tests was the EVA Walkback Test (ICES 2007-01-3133). NASA-JSC assembled a multi-disciplinary team to conduct the second test of the series, titled Integrated Suit Test 1 (IST-1), from March 6 through July 24, 2007. Similar to the Walkback Test, this study was performed with the Mark III (MKIII) EVA Technology Demonstrator suit, a treadmill, and the Partial Gravity Simulator in the Space Vehicle Mock-Up Facility at Johnson Space Center. The data collected for IST-1 included metabolic rates, ground reaction forces, biomechanics, and subjective workload and controllability feedback on both suited and unsuited (shirt-sleeve) astronaut subjects. For IST-1 the center of gravity was controlled to a nearly perfect position while the weight, pressure and biomechanics (waist locked vs. unlocked) were varied individually to evaluate the effects of each on the ability to perform level (0 degree incline) ambulation in simulated Lunar gravity. The detailed test methodology and preliminary key findings of IST-1 are summarized in this report.

  1. The Apollo Number: space suits, self-support, and the walk-run transition.

    Directory of Open Access Journals (Sweden)

    Christopher E Carr

    Full Text Available BACKGROUND: How space suits affect the preferred walk-run transition is an open question with relevance to human biomechanics and planetary extravehicular activity. Walking and running energetics differ; in reduced gravity (<0.5 g, running, unlike on Earth, uses less energy per distance than walking. METHODOLOGY/PRINCIPAL FINDINGS: The walk-run transition (denoted * correlates with the Froude Number (Fr = v(2/gL, velocity v, gravitational acceleration g, leg length L. Human unsuited Fr* is relatively constant (approximately 0.5 with gravity but increases substantially with decreasing gravity below approximately 0.4 g, rising to 0.9 in 1/6 g; space suits appear to lower Fr*. Because of pressure forces, space suits partially (1 g or completely (lunar-g support their own weight. We define the Apollo Number (Ap = Fr/M as an expected invariant of locomotion under manipulations of M, the ratio of human-supported to total transported mass. We hypothesize that for lunar suited conditions Ap* but not Fr* will be near 0.9, because the Apollo Number captures the effect of space suit self-support. We used the Apollo Lunar Surface Journal and other sources to identify 38 gait events during lunar exploration for which we could determine gait type (walk/lope/run and calculate Ap. We estimated the binary transition between walk/lope (0 and run (1, yielding Fr* (0.36+/-0.11, mean+/-95% CI and Ap* (0.68+/-0.20. CONCLUSIONS/SIGNIFICANCE: The Apollo Number explains 60% of the difference between suited and unsuited Fr*, appears to capture in large part the effects of space suits on the walk-run transition, and provides several testable predictions for space suit locomotion and, of increasing relevance here on Earth, exoskeleton locomotion. The knowledge of how space suits affect gait transitions can be used to optimize space suits for use on the Moon and Mars.

  2. Novel influenza virus vectors expressing Brucella L7/L12 or Omp16 proteins in cattle induced a strong T-cell immune response, as well as high protectiveness against B. abortus infection.

    Science.gov (United States)

    Tabynov, Kaissar; Kydyrbayev, Zhailaubay; Ryskeldinova, Sholpan; Yespembetov, Bolat; Zinina, Nadezhda; Assanzhanova, Nurika; Kozhamkulov, Yerken; Inkarbekov, Dulat; Gotskina, Tatyana; Sansyzbay, Abylai

    2014-04-11

    This paper presents the results of a study of the immunogenicity and protectiveness of new candidate vector vaccine against Brucella abortus - a bivalent vaccine formulation consisting of a mixture of recombinant influenza A subtype H5N1 or H1N1 (viral constructs vaccine formulation) viruses expressing Brucella ribosomal protein L7/L12 and Omp16, in cattle. To increase the effectiveness of the candidate vaccine, adjuvants such as Montanide Gel01 or chitosan were included in its composition. Immunization of cattle (heifers aged 1-1.5 years, 5 animals per group) with the viral constructs vaccine formulation only, or its combination with adjuvants Montanide Gel01 or chitosan, was conducted via the conjunctival method using cross prime (influenza virus subtype H5N1) and booster (influenza virus subtype H1N1) vaccination schedules at an interval of 28 days. Vaccine candidates were evaluated in comparison with the positive (B. abortus S19) and negative (PBS) controls. The viral constructs vaccine formulations, particularly in combination with Montanide Gel01 adjuvant promoted formation of IgG antibodies (with a predominance of antibodies of isotype IgG2a) against Brucella L7/L12 and Omp16 proteins in ELISA. Moreover, these vaccines in cattle induced a strong antigen-specific T-cell immune response, as indicated by a high number of CD4(+) and CD8(+) cells, as well as the concentration of IFN-γ, and most importantly provided a high level of protectiveness comparable to the commercial B. abortus S19 vaccine and superior to the B. abortus S19 vaccine in combination with Montanide Gel01 adjuvant. Based on these findings, we recommended the bivalent vaccine formulation containing the adjuvant Montanide Gel01 for practical use in cattle. Copyright © 2014 The Authors. Published by Elsevier Ltd.. All rights reserved.

  3. Walking a mile in another's shoes: The impact of wearing an Age Suit.

    Science.gov (United States)

    Lavallière, Martin; D'Ambrosio, Lisa; Gennis, Angelina; Burstein, Arielle; Godfrey, Kathryn M; Waerstad, Hilde; Puleo, Rozanne M; Lauenroth, Andreas; Coughlin, Joseph F

    2017-01-01

    The "Age Suit" described in this article was developed to enable future designers, business leaders, and engineers to experience navigating the world as many older adults must. Tools such as this Age Suit offer the opportunity to "walk a mile" in another's shoes to develop empathy that can result in better design of spaces, goods, and services to meet the needs of a rapidly growing older population. This work first examined, through a series of clinical tests, whether younger adults' physical capacities were reduced in a direction consistent with aging by wearing a suit developed by the MIT AgeLab. An experiential learning task was then completed with the suit to understand its impact on completion of an instrumental activity of daily living. Results showed that younger adults wearing the suit experienced changes in task performance consistent with expected changes associated with aging. Participants' self-reports from the experiential learning task indicated that they were able to empathize with older adults regarding some issues they face while completing a grocery shopping task. Future research with the suit should involve a wider range of individuals from the population and examine what effect participants' levels of fitness have on the experience of wearing the suit.

  4. Inherent work suit buoyancy distribution: effects on lifejacket self-righting performance.

    Science.gov (United States)

    Barwood, Martin J; Long, Geoffrey M; Lunt, Heather; Tipton, Michael J

    2014-09-01

    Accidental immersion in cold water is an occupational risk. Work suits and life jackets (LJ) should work effectively in combination to keep the airway clear of the water (freeboard) and enable self-righting. We hypothesized that inherent buoyancy, in the suit or LJ, would be beneficial for enabling freeboard, but its distribution may influence LJ self-righting. Six participants consented to complete nine immersions. Suits and LJ tested were: flotation suit (FLOAT; 85 N inherent buoyancy); oilskins 1 (OS-1) and 2 (OS-2), both with no inherent buoyancy; LJs (inherent buoyancy/buoyancy after inflation/total buoyancy), LJ-1 50/150/200 N, LJ-2 0/290/290 N, LJ-3 80/190/270 N. Once dressed, the subject entered an immersion pool where uninflated freeboard, self-righting performance, and inflated freeboard were measured. Data were compared using Friedman's test to the 0.05 alpha level. All suits and LJs enabled uninflated and inflated freeboard, but differences were seen between the suits and LJs. Self-righting was achieved on 43 of 54 occasions, irrespective of suit or LJ. On all occasions that self-righting was not achieved, this occurred in an LJ that included inherent buoyancy (11/54 occasions). Of these 11 failures, 8 occurred (73% of occasions) when the FLOAT suit was being worn. LJs that included inherent buoyancy, that are certified as effective on their own, worked less effectively from the perspective of self-righting in combination with a work suit that also included inherent buoyancy. Equipment that is approved for use in the workplace should be tested in combination to ensure adequate performance in an emergency scenario.

  5. Axial profiles of burned and fraction of holes for calculations of criticality with credit for BWR fuel burning

    International Nuclear Information System (INIS)

    Casado Sanchez, C.; Rubio Oviedo, P.

    2014-01-01

    This paper presents a method to define surround profiles of burning and fraction of holes suited for use in applications of credit to burning of BWR fuel from results obtained with the module STARBUCS of SCALE. (Author)

  6. FusionCharts Beginner's Guide The Official Guide for FusionCharts Suite

    CERN Document Server

    Nadhani, Sanket; Bhattacharya, Shamasis

    2012-01-01

    The book is written as a practical, step-by-step guide to using FusionCharts Suite. The book not only teaches you the fundamentals and implementation of FusionCharts Suite, but also makes you the data visualization guru among your friends and colleagues by teaching how to select the right chart type and usability tips. Filled with examples, code samples and practical tips in a no-nonsense way, the book is a breeze to read.This book is both for beginners and advanced web developers who need to create interactive charts for their web applications. No previous knowledge of FusionCharts Suite is a

  7. Adobe Creative Suite 6 Design and Web Premium all-in-one for dummies

    CERN Document Server

    Smith, Jennifer; Gerantabee, Fred

    2012-01-01

    The must-have book on the leading suite of software for graphic and web designers Fully revised and updated this hands-on resource offers a one-stop learning opportunity through eight mini-book dedicated to each product inside Adobe's Design & Web Premium Suite. The mini-books include Adobe Creative Suite Basics, InDesign, Illustrator, Photoshop, Acrobat, Dreamweaver, Flash, Fireworks. The book may contain new image enhancements to After Effects, 64-bit versions of Illustrator and Flash Professional, and a new tool, dubbed Helium, that will enable designers to create content using HTML5 and

  8. The use of antigravity suits in the treatment of idiopathic orthostatic hypotension

    Science.gov (United States)

    Landmark, K.; Kravik, S.

    1980-01-01

    Idiopathic orthostatic hypotension is an uncommon disease characterized by a drop in blood pressure when going from a recumbent to a standing position. Treatment by medication generally produces poor results. Three patients at the Royal Hospital in Oslo were treated with antigravity suits and all were able to maintain adequate blood pressures in the standing position. One patient improved dramatically and was able to take short walks while wearing the suit. The two other patients, however, felt that wearing the suits eventually became uncomfortable. This treatment represents a useful treatment alternative for intractable cases.

  9. Carbon Dioxide Washout Testing Using Various Inlet Vent Configurations in the Mark-III Space Suit

    Science.gov (United States)

    Korona, F. Adam; Norcross, Jason; Conger, Bruce; Navarro, Moses

    2014-01-01

    Requirements for using a space suit during ground testing include providing adequate carbon dioxide (CO2) washout for the suited subject. Acute CO2 exposure can lead to symptoms including headache, dyspnea, lethargy, and eventually unconsciousness or even death. Symptoms depend on several factors including inspired partial pressure of CO2 (ppCO2), duration of exposure, metabolic rate of the subject, and physiological differences between subjects. Computational Fluid Dynamics (CFD) analysis has predicted that the configuration of the suit inlet vent has a significant effect on oronasal CO2 concentrations. The main objective of this test was to characterize inspired oronasal ppCO2 for a variety of inlet vent configurations in the Mark-III suit across a range of workload and flow rates. Data and trends observed during testing along with refined CFD models will be used to help design an inlet vent configuration for the Z-2 space suit. The testing methodology used in this test builds upon past CO2 washout testing performed on the Z-1 suit, Rear Entry I-Suit, and the Enhanced Mobility Advanced Crew Escape Suit. Three subjects performed two test sessions each in the Mark-III suit to allow for comparison between tests. Six different helmet inlet vent configurations were evaluated during each test session. Suit pressure was maintained at 4.3 psid. Suited test subjects walked on a treadmill to generate metabolic workloads of approximately 2000 and 3000 BTU/hr. Supply airflow rates of 6 and 4 actual cubic feet per minute were tested at each workload. Subjects wore an oronasal mask with an open port in front of the mouth and were allowed to breathe freely. Oronasal ppCO2 was monitored real-time via gas analyzers with sampling tubes connected to the oronasal mask. Metabolic rate was calculated from the CO2 production measured by an additional gas analyzer at the air outlet from the suit. Real-time metabolic rate measurements were used to adjust the treadmill workload to meet

  10. Metabolic and Subjective Results Review of the Integrated Suit Test Series

    Science.gov (United States)

    Norcross, J.R.; Stroud, L.C.; Klein, J.; Desantis, L.; Gernhardt, M.L.

    2009-01-01

    Crewmembers will perform a variety of exploration and construction activities on the lunar surface. These activities will be performed while inside an extravehicular activity (EVA) spacesuit. In most cases, human performance is compromised while inside an EVA suit as compared to a crewmember s unsuited performance baseline. Subjects completed different EVA type tasks, ranging from ambulation to geology and construction activities, in different lunar analog environments including overhead suspension, underwater and 1-g lunar-like terrain, in both suited and unsuited conditions. In the suited condition, the Mark III (MKIII) EVA technology demonstrator suit was used and suit pressure and suit weight were parameters tested. In the unsuited conditions, weight, mass, center of gravity (CG), terrain type and navigation were the parameters. To the extent possible, one parameter was varied while all others were held constant. Tests were not fully crossed, but rather one parameter was varied while all others were left in the most nominal setting. Oxygen consumption (VO2), modified Cooper-Harper (CH) ratings of operator compensation and ratings of perceived exertion (RPE) were measured for each trial. For each variable, a lower value correlates to more efficient task performance. Due to a low sample size, statistical significance was not attainable. Initial findings indicate that suit weight, CG and the operational environment can have a large impact on human performance during EVA. Systematic, prospective testing series such as those performed to date will enable a better understanding of the crucial interactions of the human and the EVA suit system and their environment. However, work remains to be done to confirm these findings. These data have been collected using only unsuited subjects and one EVA suit prototype that is known to fit poorly on a large demographic of the astronaut population. Key findings need to be retested using an EVA suit prototype better suited to a

  11. Controlatron Neutron Tube Test Suite Software Manual - Operation Manual (V2.2)

    CERN Document Server

    Noel, W P; Hertrich, R J; Martinez, M L; Wallace, D L

    2002-01-01

    The Controlatron Software Suite is a custom built application to perform automated testing of Controlatron neutron tubes. The software package was designed to allowing users to design tests and to run a series of test suites on a tube. The data is output to ASCII files of a pre-defined format for data analysis and viewing with the Controlatron Data Viewer Application. This manual discusses the operation of the Controlatron Test Suite Software and a brief discussion of state machine theory, as state machine is the functional basis of the software.

  12. Geophysical validation of SCIAMACHY Limb Ozone Profiles

    Directory of Open Access Journals (Sweden)

    E. J. Brinksma

    2006-01-01

    Full Text Available We discuss the quality of the two available SCIAMACHY limb ozone profile products. They were retrieved with the University of Bremen IFE's algorithm version 1.61 (hereafter IFE, and the official ESA offline algorithm (hereafter OL versions 2.4 and 2.5. The ozone profiles were compared to a suite of correlative measurements from ground-based lidar and microwave, sondes, SAGE II and SAGE III (Stratospheric Aerosol and Gas Experiment. To correct for the expected Envisat pointing errors, which have not been corrected implicitly in either of the algorithms, we applied a constant altitude shift of -1.5 km to the SCIAMACHY ozone profiles. The IFE ozone profile data between 16 and 40 km are biased low by 3-6%. The average difference profiles have a typical standard deviation of 10% between 20 and 35 km. We show that more than 20% of the SCIAMACHY official ESA offline (OL ozone profiles version 2.4 and 2.5 have unrealistic ozone values, most of these are north of 15° S. The remaining OL profiles compare well to correlative instruments above 24 km. Between 20 and 24 km, they underestimate ozone by 15±5%.

  13. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Snow Cover Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of snow cover from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument...

  14. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Smoothed Normalized Difference Vegetation Index (NDVI) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Visible Infrared Imaging Radiometer Suite (VIIRS) Smoothed Normalized Difference Vegetation Index (NDVI) from NDE is a weekly product derived from the VIIRS...

  15. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Aerosol Detection Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains a high quality operational Environmental Data Record (EDR) of suspended matter from the Visible Infrared Imaging Radiometer Suite (VIIRS)...

  16. What was uniform about the fin-de-siècle sailor suit?

    Science.gov (United States)

    Rose, Clare

    2011-01-01

    The sailor suits widely worn by children in late-nineteenth-century Britain have been interpreted at the time, and since, as expressions of an Imperial ethos. Yet, a closer examination of the ways that these garments were produced by mass manufacturers, mediated by advertisers and fashion advisors and consumed by families makes us question this characterization. Manufacturers interpreted sailor suits not as unchanging uniforms but as fashion items responding to seasonal changes. Consumers used them to assert social identities and social distinctions, selecting from the multiple variants available. Cultural commentators described sailor suits as emulating Royal practice—but also as ‘common’ and to be avoided. A close analysis of large samples of images and texts from the period 1870–1900 reveals how these different meanings overlapped, making the fin-de-siècle sailor suit a garment that undermines many of our assumptions.

  17. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Sensor Data Record (SDR) from IDPS

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Sensor Data Records (SDRs), or Level 1b data, from the Visible Infrared Imaging Radiometer Suite (VIIRS) are the calibrated and geolocated radiance and reflectance...

  18. Corporation suit in administrative proceedings - BVerwG, NJW 1981, 362

    International Nuclear Information System (INIS)

    Skouris, W.

    1982-01-01

    The above mentioned decisions show that the repeated demand for an admission of the corporation suit has not had much impact on jurisdiction. Still the courts are examining whether the rights of corporations taking action against the executive measures are being infringed by them or not. They do not seem to be willing to admit the enforcement of members' rights or of public interests by means of a corporation suit except in those cases that are already embodied in the law. The latest statement of the administrative courts prove that the administrative procedural law (still) in force does not accept any general law on the conduct of a case on behalf of associations for the protection of their members' rights (egoistic corporation suit), nor does it acknowledge the legitimacy of corporations to see against objective illegalities in official decisions with the intention of reducing deficiencies in the execution (altruistic corporation suit). (orig.) [de

  19. Comparison of different calibration methods suited for calibration problems with many variables

    DEFF Research Database (Denmark)

    Holst, Helle

    1992-01-01

    This paper describes and compares different kinds of statistical methods proposed in the literature as suited for solving calibration problems with many variables. These are: principal component regression, partial least-squares, and ridge regression. The statistical techniques themselves do...

  20. Even at a Board Meeting, Your Immunity to Defamation Suits Is Limited.

    Science.gov (United States)

    Henderson, Donald H.

    1986-01-01

    Common law grants immunity for defamatory statements to administrative bodies such as boards. The limitations under which board participants function in relation to immunity from defamation suits are outlined. Includes discussion of specific court cases. (MD)

  1. Tartumurdelise kirjanduse ettejõudmine ; Parandatud kirikukeele ja rahvaraamatute poole / Gustav Suits

    Index Scriptorium Estoniae

    Suits, Gustav, 1883-1956

    1999-01-01

    Varem ilmunud: Suits, Gustav. Eesti kirjanduslugu I. Lund : Eesti Kirjanike Kooperatiiv, 1953. Lõunaeestikeelne "Meie Issanda Jesusse Kristusse Wastne Testament" (Riia, 1686). Eeltöödest põhjaeestikeelse piibli väljaandmiseks

  2. PROPOSED SUITE OF MODELS FOR ESTIMATING DOSE RESULTING FROM EXPOSURES BY THE DERMAL ROUTE

    Science.gov (United States)

    Recent risk assessment guidance emphasizes consideration of mechanistic factors for influencing disposition of a toxicant. To incorporate mechanistic information into risk assessment, a suite of models is proposed for use in characterizing and quantifying dosimetry of toxic age...

  3. 75 FR 24946 - Proposed Consent Decree, Clean Air Act Citizen Suit

    Science.gov (United States)

    2010-05-06

    ..., South Carolina, Wisconsin, Indiana, Michigan, Ohio, Louisiana, Kansas, Nebraska, Missouri, Colorado..., Wisconsin, Indiana, Michigan, Ohio, Louisiana, Kansas, Nebraska, Missouri, Colorado, Montana, South Dakota... ENVIRONMENTAL PROTECTION AGENCY [FRL-9146-3] Proposed Consent Decree, Clean Air Act Citizen Suit...

  4. Higher Order Thinking in the Australian Army Suite of Logistic Officer Courses

    National Research Council Canada - National Science Library

    Bradford, Scott R

    2006-01-01

    .... The current Suite of Logistic Officer Courses (SOLOC) has been recently criticized for failing to meet this requirement, with the general perception that there is a distinct lack of higher-order thinking competencies within this continuum...

  5. NGSUtils: a software suite for analyzing and manipulating next-generation sequencing datasets

    OpenAIRE

    Breese, Marcus R.; Liu, Yunlong

    2013-01-01

    Summary: NGSUtils is a suite of software tools for manipulating data common to next-generation sequencing experiments, such as FASTQ, BED and BAM format files. These tools provide a stable and modular platform for data management and analysis.

  6. NOAA JPSS Visible Infrared Imaging Radiometer Suite (VIIRS) Cloud Mask Environmental Data Record (EDR) from NDE

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains a high quality Environmental Data Record (EDR) of cloud masks from the Visible Infrared Imaging Radiometer Suite (VIIRS) instrument onboard...

  7. Assessing the Effectiveness of Defensive Aid Suite Technology Using a Field Trial and Modelling and Simulation

    National Research Council Canada - National Science Library

    Fournier, Pierre

    2002-01-01

    ...) community that Defensive Aid Suite (DAS) technologies can improve the protection of LAVs. A prototype DAS system was developed by DRDC Valcartier and tested in field trials held in 1995 and 1999...

  8. MULTIFUNCTIONAL, SELF-HEALING HYBRIDSIL MATERIALS FOR EVA SPACE SUIT PRESSURE GARMENT SYSTEMS, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — A Phase II SBIR transition of NanoSonic's high flex HybridSil space suit bladder and glove materials will provide a pivotal funding bridge toward Phase III...

  9. Closed-Loop, Non-Venting Thermal Control for Mars EVA Suits, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — NASA seeks new thermal control technology for EVA suits on Mars. The system must be closed-loop and non-venting, have negligible impact on the Martian environment,...

  10. Dynamic Human-Centered Suit Design: A Computational and Experimental Method

    Data.gov (United States)

    National Aeronautics and Space Administration — Introduction: Manned space flight necessitates an ability to provide life support to crewmembers during multiple mission stages, in the form of space suits. With...

  11. Airport Gate Activity Monitoring Tool Suite for Improved Turnaround Prediction, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — The objective of this research is to create a suite of tools for monitoring airport gate activities with the objective of improving aircraft turnaround. Airport ramp...

  12. Astronaut John Young during final suiting operations for Apollo 10 mission

    Science.gov (United States)

    1969-01-01

    A technician attaches hose from test stand to spacesuit of Astronaut John W. Young, Apollo 10 command module pilot, during final suiting operations for the Apollo 10 lunar orbit mission. Another technician makes adjustment behind Young.

  13. Compact, Efficient, and Reliable Ventilation Fan for EVA Suits, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Advanced EVA suits for space exploration will need a portable life support system (PLSS) that is compact, lightweight, and highly reliable. A key component is a...

  14. Multipurpose Cooling Garment for Improved Space Suit Environmental Control, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — Future manned space exploration missions will require space suits with capabilities beyond the current state of the art. Portable Life Support Systems for these...

  15. Compact, Efficient, and Reliable Ventilation Fan for EVA Suits, Phase II

    Data.gov (United States)

    National Aeronautics and Space Administration — Advanced EVA suits for space exploration will need a portable life support system (PLSS) that is compact, lightweight, highly reliable, and meets stringent...

  16. Evaluation of Triathlon Suit Characteristics Relevant to Thermophysiology of an Athlete

    Directory of Open Access Journals (Sweden)

    Chris Watson

    2018-02-01

    Full Text Available The thermophysiological function of clothing influences athletic wellbeing and performance, particularly in outdoor endurance activities such as triathlon. However, there is very little existing research on the performance of triathlon suits relative to thermophysiological function of the wearer. This pilot study provides a benchmark for triathlon suit performance and insights into improving the suit design and material engineering. The study assessed the thermal and breathability attributes of 6 triathlon suits and concluded that while both of the attributes were similar overall, they varied in different body zones due to different design, construction and materials. Local thermal and evaporative performance were affected by fabric construction; double fabric layering in the stomach panel; the number, size, shape and material structure of rear pockets; cycle crotch pad size, shape and thickness; and panel design. The results of this study show the importance of garment design, construction and materials for the best thermal and evaporative resistance attributes of sportswear.

  17. A Laser-Based Diagnostic Suite for Hypersonic Test Facilities, Phase I

    Data.gov (United States)

    National Aeronautics and Space Administration — In this SBIR effort, Los Gatos Research (LGR) proposes to develop a suite of laser-based diagnostics for the study of reactive and non-reactive hypersonic flows....

  18. Stravinsky: Symphony in E flat, Op. 1. Firebird-Suite (1919 version / Jonathan Swain

    Index Scriptorium Estoniae

    Swain, Jonathan

    1994-01-01

    Uuest heliplaadist "Stravinsky: Symphony in E flat, Op. 1. Firebird-Suite (1919 version). Royal Philharmonic Orchestra / Dalia Atlas; Symphony - comparative versions: SNO, Gibson. SRO, Järvi (2/94)(CHAN) CHAN 9236

  19. Microbial contamination determination of Cream suit,Traditional Ice Cream and Olovia in Yasuj City

    OpenAIRE

    SS Khoramrooz; M Sarikhani; SA Khosravani; M Farhang Falah; Y Mahmoudi; A Sharifi

    2015-01-01

    Background & aim: Prevalence of diseases caused by consumption of contaminated food has always been a problem all over the world, and every year spent on improving the disease is costly.Cream suit, Ice cream & olowye for ingredient substance and manufacture & preservation conditional have very high possibility for contamination.The aim of this study is Microbial contamination determination of Cream suit, Traditional Ice Cream and Olovia in Yasuj City Methods: This study is randomized cros...

  20. A geological investigation of the Proterozoic T'Oubep Suite, northwestern Cape Province, South Africa

    International Nuclear Information System (INIS)

    Agenbacht, A.L.D.; Schoch, A.E.; Van der Merwe, S.W.

    1990-01-01

    The T'Oubep Suite occurs in the central part of the Namaqua mobile belt approximately 80km southwest of Kakamas. The Suite comprises seven different rock types with compositions ranging from betabasite through granodiorite and tonalite to granite. The igneous rocks were extensively metamorphosed during the Namaqua tectogenesis. The comagmatic origin for the granitic rocks suggested by the field relations is corroborated by whole rock, trace element and REE chemistry. 5 refs., 3 figs

  1. New isotope data from the Koperberg Suite and some associated rocks, Okiep district, Namaqualand, South Africa

    International Nuclear Information System (INIS)

    Clifford, T.N.; Barton, E.S.; Retief, E.A.; Rex, D.C.

    1990-01-01

    The Koperberg Suite is an anorthosite-charnockite kindred and 1500 bodies of these rocks have been recognised in the Okiep district and 30 of them have been mined for copper since 1852. The suite is intrusive into country rocks. New isotope data presented includes U-Pb zircon and whole-rock Pb-Pb, Rb-Sr, Sm-Nd and Ar-Ar analyses. 10 refs

  2. Extreme-Environment Silicon-Carbide (SiC) Wireless Sensor Suite

    Science.gov (United States)

    Yang, Jie

    2015-01-01

    Phase II objectives: Develop an integrated silicon-carbide wireless sensor suite capable of in situ measurements of critical characteristics of NTP engine; Compose silicon-carbide wireless sensor suite of: Extreme-environment sensors center, Dedicated high-temperature (450 deg C) silicon-carbide electronics that provide power and signal conditioning capabilities as well as radio frequency modulation and wireless data transmission capabilities center, An onboard energy harvesting system as a power source.

  3. Time Management in the Operating Room: An Analysis of the Dedicated Minimally Invasive Surgery Suite

    Science.gov (United States)

    Hsiao, Kenneth C.; Machaidze, Zurab

    2004-01-01

    Background: Dedicated minimally invasive surgery suites are available that contain specialized equipment to facilitate endoscopic surgery. Laparoscopy performed in a general operating room is hampered by the multitude of additional equipment that must be transported into the room. The objective of this study was to compare the preparation times between procedures performed in traditional operating rooms versus dedicated minimally invasive surgery suites to see whether operating room efficiency is improved in the specialized room. Methods: The records of 50 patients who underwent laparoscopic procedures between September 2000 and April 2002 were retrospectively reviewed. Twenty-three patients underwent surgery in a general operating room and 18 patients in an minimally invasive surgery suite. Nine patients were excluded because of cystoscopic procedures undergone prior to laparoscopy. Various time points were recorded from which various time intervals were derived, such as preanesthesia time, anesthesia induction time, and total preparation time. A 2-tailed, unpaired Student t test was used for statistical analysis. Results: The mean preanesthesia time was significantly faster in the minimally invasive surgery suite (12.2 minutes) compared with that in the traditional operating room (17.8 minutes) (P=0.013). Mean anesthesia induction time in the minimally invasive surgery suite (47.5 minutes) was similar to time in the traditional operating room (45.7 minutes) (P=0.734). The average total preparation time for the minimally invasive surgery suite (59.6 minutes) was not significantly faster than that in the general operating room (63.5 minutes) (P=0.481). Conclusion: The amount of time that elapses between the patient entering the room and anesthesia induction is statically shorter in a dedicated minimally invasive surgery suite. Laparoscopic surgery is performed more efficiently in a dedicated minimally invasive surgery suite versus a traditional operating room. PMID

  4. G SUIT FOR EDUCATION AS AN ENVIRONMENT FOR STUDENTS OF EDUCATIONAL PRACTICES

    OpenAIRE

    Olena Glazunova; Olena Kuzminska; Tetyana Voloshyna; Taisia Sayapina; Valentyna Korolchuk

    2017-01-01

    Materials article analyzes the opportunities and benefits of using cloud services G Suite (Google Apps) during the practical training of students. In the course of the study, an e-environment based on G Suit for Education was developed and its use for effective organization of information technology practice training. A model of e-learning environment for organizing group project work in Google Classroom-based training has been built. The design methodology is grounded as one of the most effe...

  5. Human skin in vitro permeation of bentazon and isoproturon formulations with or without protective clothing suit.

    Science.gov (United States)

    Berthet, Aurélie; Hopf, Nancy B; Miles, Alexandra; Spring, Philipp; Charrière, Nicole; Garrigou, Alain; Baldi, Isabelle; Vernez, David

    2014-01-01

    Skin exposures to chemicals may lead, through percutaneous permeation, to a significant increase in systemic circulation. Skin is the primary route of entry during some occupational activities, especially in agriculture. To reduce skin exposures, the use of personal protective equipment (PPE) is recommended. PPE efficiency is characterized as the time until products permeate through material (lag time, Tlag). Both skin and PPE permeations are assessed using similar in vitro methods; the diffusion cell system. Flow-through diffusion cells were used in this study to assess the permeation of two herbicides, bentazon and isoproturon, as well as four related commercial formulations (Basagran(®), Basamais(®), Arelon(®) and Matara(®)). Permeation was measured through fresh excised human skin, protective clothing suits (suits) (Microchem(®) 3000, AgriSafe Pro(®), Proshield(®) and Microgard(®) 2000 Plus Green), and a combination of skin and suits. Both herbicides, tested by itself or as an active ingredient in formulations, permeated readily through human skin and tested suits (Tlag < 2 h). High permeation coefficients were obtained regardless of formulations or tested membranes, except for Microchem(®) 3000. Short Tlag, were observed even when skin was covered with suits, except for Microchem(®) 3000. Kp values tended to decrease when suits covered the skin (except when Arelon(®) was applied to skin covered with AgriSafe Pro and Microgard(®) 2000), suggesting that Tlag alone is insufficient in characterizing suits. To better estimate human skin permeations, in vitro experiments should not only use human skin but also consider the intended use of the suit, i.e., the active ingredient concentrations and type of formulations, which significantly affect skin permeation.

  6. Russian contribution to ExoMars Trace Gas Orbiter: Atmospheric Chemistry Suite (ACS)

    Science.gov (United States)

    Shakun, Alexey; Korablev, Oleg; Trokhimovskiy, Alexander; Grigoriev, Alexey; Anufreychik, Konstantin; Fedorova, Anna; Ignatiev, Nikolay; Ivanov, Yuriy; Moshkin, Boris; Kalinnikov, Yuriy; Montmessin, Franck

    2016-04-01

    Atmospheric Chemistry Suite (ACS) is a part of science payload of Trace Gas Orbiter (TGO), ExoMars mission. This project developed by European Space Agency (ESA) in collaboration with Russian Space Agency (Roscosmos). Russian contribution to ExoMars TGO is the Proton rocket and two science instruments ACS (three infrared spectrometers) and FREND (neutron detector). ACS consists of three infrared spectrometers (ACS/NIR, ACS/MIR and ACS/TIRVIM) capable to take spectral measurements from near to thermal infrared range simultaneously or separately. Spectrometric channels of ACS share common mechanical, electrical, and thermal interfaces. Electronic box (ACS/BE) provides to spectrometric channels power and data transfer interfaces. SpaceWire link is used for science data transfer and MIL-1553 link - for commanding and housekeeping data transfer. The NIR channel is an echelle spectrometer with acousto-optic tunable filter (AOTF) for the selection of diffraction orders. ACS NIR is capable to perform nadir and occultation observations. NIR covers the spectral range of 0.7-1.7 μm with resolving power of ~25000. NIR will perform unique for TGO instruments nightglow science (searching for O2, OH, NO nightglow emissions on Mars). From the 1.38 μm band NIR will do water vapour mapping in nadir and H2O vertical profiling in solar occultations. High resolution NIR measurements of 1.27 μm O2(a1Δg) dayglow will supply indirect ozone observations on the dayside on nadir. In solar occultation mode, the O2 vertical profiles will be measured from the surface (in case of low dust activity) to the 40 km altitude based on 0.76 μm absorption band. Together with MIR channel in solar occultation NIR will support the measurements of CO2 density profiles (based on 1.43 μm band) and aerosols characterization from 0.7 to 4 μm. The wide spectral range will allow not just determine aerosol particle sizes and density at different altitudes, but also distinguish between dust and ice particles

  7. Post-collisional magmatism in the central East African Orogen: The Maevarano Suite of north Madagascar

    Science.gov (United States)

    Goodenough, K.M.; Thomas, Ronald J.; De Waele, B.; Key, R.M.; Schofield, D.I.; Bauer, W.; Tucker, R.D.; Rafahatelo, J.-M.; Rabarimanana, M.; Ralison, A.V.; Randriamananjara, T.

    2010-01-01

    Late tectonic, post-collisional granite suites are a feature of many parts of the Late Neoproterozoic to Cambrian East African Orogen (EAO), where they are generally attributed to late extensional collapse of the orogen, accompanied by high heat flow and asthenospheric uprise. The Maevarano Suite comprises voluminous plutons which were emplaced in some of the tectonostratigraphic terranes of northern Madagascar, in the central part of the EAO, following collision and assembly during a major orogeny at ca. 550 Ma. The suite comprises three main magmatic phases: a minor early phase of foliated gabbros, quartz diorites, and granodiorites; a main phase of large batholiths of porphyritic granitoids and charnockites; and a late phase of small-scale plutons and sheets of monzonite, syenite, leucogranite and microgranite. The main phase intrusions tend to be massive, but with variably foliated margins. New U-Pb SHRIMP zircon data show that the whole suite was emplaced between ca. 537 and 522 Ma. Geochemically, all the rocks of the suite are enriched in the LILE, especially K, and the LREE, but are relatively depleted in Nb, Ta and the HREE. These characteristics are typical of post-collisional granitoids in the EAO and many other orogenic belts. It is proposed that the Maevarano Suite magmas were derived by melting of sub-continental lithospheric mantle that had been enriched in the LILE during earlier subduction events. The melting occurred during lithospheric delamination, which was associated with extensional collapse of the East African Orogen. ?? 2009 Natural Environment Research Council.

  8. Z-2 Space Suit: A Case Study in Human Spaceflight Public Outreach

    Science.gov (United States)

    McFarland, Shane M.

    2016-01-01

    NASA Johnson Space Center's Z-series of planetary space suit prototypes is an iterative development platform with a Mars-forward design philosophy, targeting a Mars surface mission in the mid-2030s. The first space suit assembly, called the Z-1, was delivered in 2012. While meeting the project's stated requirements and objectives, the general public's reception primarily focused on the color scheme, which vaguely invoked similarity to a certain animated cartoon character. The public at large has and continues to be exposed to varying space suit design aesthetics from popular culture and low TRL technology maturation efforts such as mechanical counterpressure. The lesson learned was that while the design aesthetic is not important from an engineering perspective, the perception of the public is important for NASA and human spaceflight in general. For the Z-2 space suit, an integrated public outreach strategy was employed to engage, excite and educate the public on the current technology of space suits and NASA's plans moving forward. The keystone of this strategy was a public vote on three different suit cover layer aesthetics, the winner of which would be used as inspiration in fabrication. Other components included social media, university collaboration, and select media appearances, the cumulative result of which, while intangible in its benefit, was ultimately a positive effect in terms of the image of NASA as well as the dissemination of information vital to dispelling public misconceptions.

  9. A method of evaluating efficiency during space-suited work in a neutral buoyancy environment

    Science.gov (United States)

    Greenisen, Michael C.; West, Phillip; Newton, Frederick K.; Gilbert, John H.; Squires, William G.

    1991-01-01

    The purpose was to investigate efficiency as related to the work transmission and the metabolic cost of various extravehicular activity (EVA) tasks during simulated microgravity (whole body water immersion) using three space suits. Two new prototype space station suits, AX-5 and MKIII, are pressurized at 57.2 kPa and were tested concurrently with the operationally used 29.6 kPa shuttle suit. Four male astronauts were asked to perform a fatigue trial on four upper extremity exercises during which metabolic rate and work output were measured and efficiency was calculated in each suit. The activities were selected to simulate actual EVA tasks. The test article was an underwater dynamometry system to which the astronauts were secured by foot restraints. All metabolic data was acquired, calculated, and stored using a computerized indirect calorimetry system connected to the suit ventilation/gas supply control console. During the efficiency testing, steady state metabolic rate could be evaluated as well as work transmitted to the dynamometer. Mechanical efficiency could then be calculated for each astronaut in each suit performing each movement.

  10. Awake craniotomy for gliomas in a high-field intraoperative magnetic resonance imaging suite: analysis of 42 cases.

    Science.gov (United States)

    Maldaun, Marcos V C; Khawja, Shumaila N; Levine, Nicholas B; Rao, Ganesh; Lang, Frederick F; Weinberg, Jeffrey S; Tummala, Sudhakar; Cowles, Charles E; Ferson, David; Nguyen, Anh-Thuy; Sawaya, Raymond; Suki, Dima; Prabhu, Sujit S

    2014-10-01

    The object of this study was to describe the experience of combining awake craniotomy techniques with high-field (1.5 T) intraoperative MRI (iMRI) for tumors adjacent to eloquent cortex. From a prospective database the authors obtained and evaluated the records of all patients who had undergone awake craniotomy procedures with cortical and subcortical mapping in the iMRI suite. The integration of these two modalities was assessed with respect to safety, operative times, workflow, extent of resection (EOR), and neurological outcome. Between February 2010 and December 2011, 42 awake craniotomy procedures using iMRI were performed in 41 patients for the removal of intraaxial tumors. There were 31 left-sided and 11 right-sided tumors. In half of the cases (21 [50%] of 42), the patient was kept awake for both motor and speech mapping. The mean duration of surgery overall was 7.3 hours (range 4.0-13.9 hours). The median EOR overall was 90%, and gross-total resection (EOR ≥ 95%) was achieved in 17 cases (40.5%). After viewing the first MR images after initial resection, further resection was performed in 17 cases (40.5%); the mean EOR in these cases increased from 56% to 67% after further resection. No deficits were observed preoperatively in 33 cases (78.5%), and worsening neurological deficits were noted immediately after surgery in 11 cases (26.2%). At 1 month after surgery, however, worsened neurological function was observed in only 1 case (2.3%). There was a learning curve with regard to patient positioning and setup times, although it did not adversely affect patient outcomes. Awake craniotomy can be safely performed in a high-field (1.5 T) iMRI suite to maximize tumor resection in eloquent brain areas with an acceptable morbidity profile at 1 month.

  11. Radiation Protection Studies of International Space Station Extravehicular Activity Space Suits

    Science.gov (United States)

    Cucinotta, Francis A. (Editor); Shavers, Mark R. (Editor); Saganti, Premkumar B. (Editor); Miller, Jack (Editor)

    2003-01-01

    This publication describes recent investigations that evaluate radiation shielding characteristics of NASA's and the Russian Space Agency's space suits. The introduction describes the suits and presents goals of several experiments performed with them. The first chapter provides background information about the dynamic radiation environment experienced at ISS and summarized radiation health and protection requirements for activities in low Earth orbit. Supporting studies report the development and application of a computer model of the EMU space suit and the difficulty of shielding EVA crewmembers from high-energy reentrant electrons, a previously unevaluated component of the space radiation environment. Chapters 2 through 6 describe experiments that evaluate the space suits' radiation shielding characteristics. Chapter 7 describes a study of the potential radiological health impact on EVA crewmembers of two virtually unexamined environmental sources of high-energy electrons-reentrant trapped electrons and atmospheric albedo or "splash" electrons. The radiological consequences of those sources have not been evaluated previously and, under closer scrutiny. A detailed computational model of the shielding distribution provided by components of the NASA astronauts' EMU is being developed for exposure evaluation studies. The model is introduced in Chapters 8 and 9 and used in Chapter 10 to investigate how trapped particle anisotropy impacts female organ doses during EVA. Chapter 11 presents a review of issues related to estimating skin cancer risk form space radiation. The final chapter contains conclusions about the protective qualities of the suit brought to light form these studies, as well as recommendations for future operational radiation protection.

  12. Ergonomic assessment of neck posture in the minimally invasive surgery suite during laparoscopic cholecystectomy.

    Science.gov (United States)

    van Det, M J; Meijerink, W J H J; Hoff, C; van Veelen, M A; Pierie, J P E N

    2008-11-01

    With the expanding implementation of minimally invasive surgery, the operating team is confronted with challenges in the field of ergonomics. Visual feedback is derived from a monitor placed outside the operating field. This crossover trial was conducted to evaluate and compare neck posture in relation to monitor position in a dedicated minimally invasive surgery (MIS) suite and a conventional operating room. Assessment of the neck was conducted for 16 surgeons, assisting surgeons, and scrub nurses performing a laparoscopic cholecystectomy in both types of operating room. Flexion and rotation of the cervical spine were measured intraoperatively using a video analysis system. A two-question visual analog scale (VAS) questionnaire was used to evaluate posture in relation to the monitor position. Neck rotation was significantly reduced in the MIS suite for the surgeon (p = 0.018) and the assisting surgeon (p < 0.001). Neck flexion was significantly improved in the MIS suite for the surgeon (p < 0.001) and the scrub nurse (p = 0.018). On the questionnaire, the operating room team scored their posture significantly higher in the MIS suite and also indicated fewer musculoskeletal complaints. The ergonomic quality of the neck posture is significantly improved in the MIS suite for the entire operating room team.

  13. The CMSSW benchmarking suite: Using HEP code to measure CPU performance

    International Nuclear Information System (INIS)

    Benelli, G

    2010-01-01

    The demanding computing needs of the CMS experiment require thoughtful planning and management of its computing infrastructure. A key factor in this process is the use of realistic benchmarks when assessing the computing power of the different architectures available. In recent years a discrepancy has been observed between the CPU performance estimates given by the reference benchmark for HEP computing (SPECint) and actual performances of HEP code. Making use of the CPU performance tools from the CMSSW performance suite, comparative CPU performance studies have been carried out on several architectures. A benchmarking suite has been developed and integrated in the CMSSW framework, to allow computing centers and interested third parties to benchmark architectures directly with CMSSW. The CMSSW benchmarking suite can be used out of the box, to test and compare several machines in terms of CPU performance and report with the wanted level of detail the different benchmarking scores (e.g. by processing step) and results. In this talk we describe briefly the CMSSW software performance suite, and in detail the CMSSW benchmarking suite client/server design, the performance data analysis and the available CMSSW benchmark scores. The experience in the use of HEP code for benchmarking will be discussed and CMSSW benchmark results presented.

  14. An integrative variant analysis suite for whole exome next-generation sequencing data

    Directory of Open Access Journals (Sweden)

    Challis Danny

    2012-01-01

    Full Text Available Abstract Background Whole exome capture sequencing allows researchers to cost-effectively sequence the coding regions of the genome. Although the exome capture sequencing methods have become routine and well established, there is currently a lack of tools specialized for variant calling in this type of data. Results Using statistical models trained on validated whole-exome capture sequencing data, the Atlas2 Suite is an integrative variant analysis pipeline optimized for variant discovery on all three of the widely used next generation sequencing platforms (SOLiD, Illumina, and Roche 454. The suite employs logistic regression models in conjunction with user-adjustable cutoffs to accurately separate true SNPs and INDELs from sequencing and mapping errors with high sensitivity (96.7%. Conclusion We have implemented the Atlas2 Suite and applied it to 92 whole exome samples from the 1000 Genomes Project. The Atlas2 Suite is available for download at http://sourceforge.net/projects/atlas2/. In addition to a command line version, the suite has been integrated into the Genboree Workbench, allowing biomedical scientists with minimal informatics expertise to remotely call, view, and further analyze variants through a simple web interface. The existing genomic databases displayed via the Genboree browser also streamline the process from variant discovery to functional genomics analysis, resulting in an off-the-shelf toolkit for the broader community.

  15. An improved air-supplied plastic suit for protection against tritium

    International Nuclear Information System (INIS)

    Wiernicki, C.

    1987-01-01

    A newly developed Saran/CPE plastic suit material is described which offers significantly better protection against HTO penetration and permeation than the 12-mil PVC currently used at SRP and most other DOE and commercial sites where tritium and HTO are exposure hazards. Tritium breakthrough time is an important parameter when evaluating the applicability of protective clothing; previously published tritium permeation tests did not measure this parameter. Future studies should quantify steady-state permeation rate and breakthrough time to more fully evaluate potential tritium protective clothing. Saran/CPE has successfully been fabricated into a plastic suit because, in addition to its superior tritium resistance, it has all the characteristics required to construct a rugged, dependable, and comfortable suit. The use of the Saran/CPE suit at SRP reactor and tritium production facilities should be a major contribution to the site As Low As Reasonably Achievable program. Both Saran/CPE have demonstrated excellent resistance to a wide range of chemical contaminants; therefore, this suit material may have applications in the general chemical industry and hazardous waste site cleanup operations. 4 refs., 3 figs., 1 tab

  16. Improved airline-type supplied-air plastic suit. [For personnel protection against inhalation of airborne plutonium and tritium

    Energy Technology Data Exchange (ETDEWEB)

    Jolley, L. Jr.; Zippler, D.B.; Cofer, C.H.; Harper, J.A.

    1978-06-01

    Two piece supplied-air plastic suits are used extensively at the Savannah River Plant for personnel protection against inhalation of airborne plutonium and tritium. Worker comfort and noise level problems gave impetus to development of an improved suit and aid distribution system. The resulting plastic suit and development work are discussed. The plastic suit unit cost is less than $20, the hearing zone noise level is less than 75 dBA, protection factors exceed 10,000, and user comfort is approved. This suit is expected to meet performance requirements for unrestricted use.

  17. Profile: Asian Americans

    Science.gov (United States)

    ... and Data > Minority Population Profiles > Asian American Profile: Asian Americans Asian American Profile (Map of the US with the top 10 states displaying the largest Asian American population according to the Census Bureau) CA - ...

  18. An overview of recent projects to study thermal protection in life rafts, lifeboats and immersion suits

    Energy Technology Data Exchange (ETDEWEB)

    Mak, L.; DuCharme, M. B.; Farnworth, B.; Wissler, E. H.; Brown, R.; Kuczora, A. [Maritime and Arctic Survival Scientific and Engineering Ressearch Team (Canada)

    2011-07-01

    Survival during a marine evacuation in cold regions is very challenging. However international regulations do not require specific thermal protection or ventilation performance criteria for lifeboats. In the same way, the testing methods for approval testing of immersion suits are not standardised. This paper investigated recent projects completed or on-going to study thermal protection in life rafts, lifeboats and immersion suits. An overview of several projects from the Maritime and Arctic Survival Scientific and Engineering Research Team (MASSERT) was conducted. This review provided the necessary knowledge to advance international standards and develop the thermal protection requirements for survival in the Arctic. The results showed the MASSERT correlated thermal insulation values between human subjects and thermal manikins in life rafts and in immersion suits. It was found that the manikins are a valuable evaluation tool, as well as the computerised models used as prediction tools.

  19. Fabrication and performance analysis of a DEA cuff designed for dry-suit applications

    International Nuclear Information System (INIS)

    Ahmadi, S; Camacho Mattos, A; Barbazza, A; Soleimani, M; Boscariol, P; Menon, C

    2013-01-01

    A method for manufacturing a cylindrical dielectric elastomer actuator (DEA) is presented. The cylindrical DEA can be used in fabricating the cuff area of dry-suits where the garment is very tight and wearing the suit is difficult. When electrically actuated, the DEA expands radially and the suit can be worn more comfortably. In order to study the performance of the DEA, a customized testing setup was designed, and silicone-made cuff samples with different material stiffnesses were tested. Analytical and FEM modeling were considered to evaluate the experimental output. The results revealed that although the stiffness of the DEA material has a direct relationship with the radial constrictive pressure caused by mechanically stretching the DEA, it has a minor effect on the actuation pressure. It was also found that stacking multiple layers of the DEA to fabricate a laminated structure enabled the attainment of a desired variation of pressure required for the implementation of an electrically tunable cuff. (paper)

  20. Experimental Evaluation of Energy Efficiency for a Soft Wearable Robotic Suit.

    Science.gov (United States)

    Jin, Shanhai; Iwamoto, Noriyasu; Hashimoto, Kazunobu; Yamamoto, Motoji

    2016-10-12

    This paper presents a new soft wearable robotic suit for energy-efficient walking in daily activities for elderly persons. The presented robotic suit provides a small yet effective assistive force for hip flexion through winding belts that include elastic elements. In addition, it does not restrict the range of movement in the lower limbs. Moreover, its structure is simple and lightweight, and thus wearers can easily take the device on and off by themselves. Experimental results on nine elderly subjects (age = 74.23.7 years) show that the robotic suit worn and powered on (PON) significantly reduced energy expenditure by an average of 5.9 % compared with the condition of worn but powered off (POFF). Furthermore, compared with the POFF condition, there was a significant improvement in gait characteristics in the PON condition for all subjects.

  1. Ventilation Transport Trade Study for Future Space Suit Life Support Systems

    Science.gov (United States)

    Kempf, Robert; Vogel, Matthew; Paul, Heather L.

    2008-01-01

    A new and advanced portable life support system (PLSS) for space suit surface exploration will require a durable, compact, and energy efficient system to transport the ventilation stream through the space suit. Current space suits used by NASA circulate the ventilation stream via a ball-bearing supported centrifugal fan. As NASA enters the design phase for the next generation PLSS, it is necessary to evaluate available technologies to determine what improvements can be made in mass, volume, power, and reliability for a ventilation transport system. Several air movement devices already designed for commercial, military, and space applications are optimized in these areas and could be adapted for EVA use. This paper summarizes the efforts to identify and compare the latest fan and bearing technologies to determine candidates for the next generation PLSS.

  2. The audio and visual communication systems for suited engineering activities on JET

    Energy Technology Data Exchange (ETDEWEB)

    Pearce, R.J.H. E-mail: robert.pearce@jet.uk; Bruce, J.; Callaghan, C.; Hart, M.; Martin, P.; Middleton, R.; Tait, J

    2001-11-01

    The beryllium and/or tritium contamination of the JET tokamak and auxiliary systems necessitates that many activities are carried out in air line fed pressurised suits. To enable often complex engineering activities to be performed, a number of novel audio and visual and communications systems have been designed. The paper describes these systems which give freedom of visual and audio communication between suited personnel, supervisors, operators and engineers. The system enhances the safety of the working environment as well as helping to minimise the radiation dose to personnel. It is concluded, from a number of years experience of using the audio and visual communications systems for suited operations, that safety and the progress of complex engineering tasks have been significantly enhanced.

  3. Equation-of-State Test Suite for the DYNA3D Code

    Energy Technology Data Exchange (ETDEWEB)

    Benjamin, Russell D. [Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

    2015-11-05

    This document describes the creation and implementation of a test suite for the Equationof- State models in the DYNA3D code. A customized input deck has been created for each model, as well as a script that extracts the relevant data from the high-speed edit file created by DYNA3D. Each equation-of-state model is broken apart and individual elements of the model are tested, as well as testing the entire model. The input deck for each model is described and the results of the tests are discussed. The intent of this work is to add this test suite to the validation suite presently used for DYNA3D.

  4. The audio and visual communication systems for suited engineering activities on JET

    International Nuclear Information System (INIS)

    Pearce, R.J.H.; Bruce, J.; Callaghan, C.; Hart, M.; Martin, P.; Middleton, R.; Tait, J.

    2001-01-01

    The beryllium and/or tritium contamination of the JET tokamak and auxiliary systems necessitates that many activities are carried out in air line fed pressurised suits. To enable often complex engineering activities to be performed, a number of novel audio and visual and communications systems have been designed. The paper describes these systems which give freedom of visual and audio communication between suited personnel, supervisors, operators and engineers. The system enhances the safety of the working environment as well as helping to minimise the radiation dose to personnel. It is concluded, from a number of years experience of using the audio and visual communications systems for suited operations, that safety and the progress of complex engineering tasks have been significantly enhanced

  5. Defining Constellation Suit Helmet Field of View Requirements Employing a Mission Segment Based Reduction Process

    Science.gov (United States)

    McFarland, Shane

    2009-01-01

    Field of view has always been a design feature paramount to helmets, and in particular space suits, where the helmet must provide an adequate field of view for a large range of activities, environments, and body positions. For Project Constellation, a different approach to helmet requirement maturation was utilized; one that was less a direct function of body position and suit pressure and more a function of the mission segment in which the field of view will be required. Through taxonimization of various parameters that affect suited field of view, as well as consideration for possible nominal and contingency operations during that mission segment, a reduction process was employed to condense the large number of possible outcomes to only six unique field of view angle requirements that still captured all necessary variables while sacrificing minimal fidelity.

  6. The European space suit, a design for productivity and crew safety

    Science.gov (United States)

    Skoog, A. Ingemar; Berthier, S.; Ollivier, Y.

    In order to fulfil the two major mission objectives, i.e. support planned and unplanned external servicing of the COLUMBUS FFL and support the HERMES vehicle for safety critical operations and emergencies, the European Space Suit System baseline configuration incorporates a number of design features, which shall enhance the productivity and the crew safety of EVA astronauts. The work in EVA is today - and will be for several years - a manual work. Consequently, to improve productivity, the first challenge is to design a suit enclosure which minimizes movement restrictions and crew fatigue. It is covered by the "ergonomic" aspect of the suit design. Furthermore, it is also necessary to help the EVA crewmember in his work, by giving him the right information at the right time. Many solutions exist in this field of Man-Machine Interface, from a very simple system, based on cuff check lists, up to advanced systems, including Head-Up Displays. The design concept for improved productivity encompasses following features: • easy donning/doffing thru rear entry, • suit ergonomy optimisation, • display of operational information in alpha-numerical and graphical from, and • voice processing for operations and safety critical information. Concerning crew safety the major design features are: • a lower R-factor for emergency EVA operations thru incressed suit pressure, • zero prebreath conditions for normal operations, • visual and voice processing of all safety critical functions, and • an autonomous life support system to permit unrestricted operations around HERMES and the CFFL. The paper analyses crew safety and productivity criteria and describes how these features are being built into the design of the European Space Suit System.

  7. Monitoring Human Performance During Suited Operations: A Technology Feasibility Study Using EMU Gloves

    Science.gov (United States)

    Bekdash, Omar; Norcross, Jason; McFarland, Shane

    2015-01-01

    Mobility tracking of human subjects while conducting suited operations still remains focused on the external movement of the suit and little is known about the human movement within it. For this study, accelerometers and bend sensitive resistors were integrated into a custom carrier glove to quantify range of motion and dexterity from within the pressurized glove environment as a first stage feasibility study of sensor hardware, integration, and reporting capabilities. Sensors were also placed on the exterior of the pressurized glove to determine if it was possible to compare a glove joint angle to the anatomical joint angle of the subject during tasks. Quantifying human movement within the suit was feasible, with accelerometers clearly detecting movements in the wrist and reporting expected joint angles at maximum flexion or extension postures with repeatability of plus or minus 5 degrees between trials. Bend sensors placed on the proximal interphalangeal and distal interphalangeal joints performed less well. It was not possible to accurately determine the actual joint angle using these bend sensors, but these sensors could be used to determine when the joint was flexed to its maximum and provide a general range of mobility needed to complete a task. Further work includes additional testing with accelerometers and the possible inclusion of hardware such as magnetometers or gyroscopes to more precisely locate the joint in 3D space. We hope to eventually expand beyond the hand and glove and develop a more comprehensive suit sensor suite to characterize motion across more joints (knee, elbow, shoulder, etc.) and fully monitor the human body operating within the suit environment.

  8. Effect of swimming suit design on the energy demands of swimming.

    Science.gov (United States)

    Starling, R D; Costill, D L; Trappe, T A; Jozsi, A C; Trappe, S W; Goodpaster, B H

    1995-07-01

    Eight competitive male swimmers completed a standardized 365.8 m (400 yd) freestyle swimming trial at a fixed pace (approximately 90% of maximal effort) while wearing a torso swim suit (TOR) or a standard racing suit (STD). Oxygen uptake (VO2), blood lactate, heart rate (HR), and distance per stroke (DPS) measurements were obtained. In addition, a video-computer system was used to collect velocity data during a prone underwater glide following a maximal leg push-off from the side of the pool while wearing the TOR and STD suits. These data were used to calculate the total distance covered during the glides. VO2 (3.76 +/- 0.16 vs 3.92 +/- 0.18 l.min-1) and lactate (8.08 +/- 0.53 vs, 9.66 +/- 0.66 mM) were significantly (P 0.05) between the TOR (170.1 +/- 5.1 b.min-1) and STD (173.5 +/- 5.7 b.min-1) trials. DPS was significantly greater during the TOR (2.70 +/- 0.066 m.stroke-1) versus STD (2.58 +/- 0.054 m.stroke-1) trial. A significantly greater total distance was covered during the prone glide while wearing the TOR (2.05 +/- 0.067 m) compared to the STD (2.00 +/- 0.080 m) suit. These findings demonstrate that a specially designed torso suit reduces the energy demand of swimming compared to a standard racing suit which may be due to a reduction in body drag.

  9. The decommissioning and demolition of four suites of high active chemical analysis cells at DNPDE

    International Nuclear Information System (INIS)

    Black, R.M.; Spence, B.W.; Stewart, C.W.

    1987-11-01

    The decommissioning and demolition of four laboratory suites of high active cells at DNPDE is described. All four suites had suffered drain leaks of high active liquor into underfloor ducts; the options available at the time and current policy for dealing with the resultant activity deposits are given. The decommissioning procedures are detailed to provide information for future similar exercises. Features to ease demolition of such facilities and to eliminate the possibility of long term activity deposition from drain leaks are highlighted for incorporation in future designs. The waste arisings and radiation doses received during the work are tabulated. (author)

  10. Specifications and test procedures for airline-type supplied-air suits

    International Nuclear Information System (INIS)

    Revoir, W.H.; Pritchard, J.A.; Davis, T.O.; Richards, C.P.; Wheat, L.D.

    1975-05-01

    Procedures and requirements have been established to permit airline-type supplied-air suits needed by contractors of the Energy Research and Development Administration to be tested for performance by the Respirator Research and Development Section, Industrial Hygiene Group, of the Los Alamos Scientific Laboratory, and to have the adequacy of the performance of these devices evaluated by the Los Alamos Scientific Laboratory Respirator Advisory Committee. Test equipment, test methods, and performance criteria for airline-type supplied-air suits are prescribed. (U.S.)

  11. Aerogel Use as a Skin Protective Liner In Space Suits and Prosthetic Limbs Project

    Science.gov (United States)

    Roberson, Luke Bennett

    2014-01-01

    Existing materials for prosthetic liners tend to be thick and airtight, causing perspiration to accumulate inside the liner and potentially causing infection and injury. The purpose of this project was to examine the suitability of aerogel for prosthetic liner applications for use in space suits and orthopedics. Three tests were performed on several types of aerogel to assess the properties of each material, and our initial findings demonstrated that these materrials would be excellent candidates for liner applications for prosthetics and space suits. The project is currently on hold until additional funding is obtained for application testing at the VH Hospitals in Tampa

  12. Multiple marker abundance profiling

    DEFF Research Database (Denmark)

    Hooper, Cornelia M.; Stevens, Tim J.; Saukkonen, Anna

    2017-01-01

    proteins and the scoring accuracy of lower-abundance proteins in Arabidopsis. NPAS was combined with subcellular protein localization data, facilitating quantitative estimations of organelle abundance during routine experimental procedures. A suite of targeted proteomics markers for subcellular compartment...

  13. Optimizing the ATLAS code with different profilers

    CERN Document Server

    Kama, S; The ATLAS collaboration

    2013-01-01

    After the current maintenance period, the LHC will provide higher energy collisions with increased luminosity. In order to keep up with these higher rates, ATLAS software needs to speed up substantially. However, ATLAS code is composed of approximately 4M lines, written by many different programmers with different backgrounds, which makes code optimisation a challenge. To help with this effort different profiling tools and techniques are being used. These include well known tools, such as the Valgrind suite and Intel Amplifier; less common tools like PIN, PAPI, and GOODA; as well as techniques such as library interposing. In this talk we will mainly focus on PIN tools and GOODA. PIN is a dynamic binary instrumentation tool which can obtain statistics such as call counts, instruction counts and interrogate functions' arguments. It has been used to obtain CLHEP Matrix profiles, operations and vector sizes for linear algebra calculations which has provided the insight necessary to achieve significant performance...

  14. Asteroid Redirect Crewed Mission Space Suit and EVA System Architecture Trade Study

    Science.gov (United States)

    Bowie, Jonathan; Buffington, Jesse; Hood, Drew; Kelly, Cody; Naids, Adam; Watson, Richard; Blanco, Raul; Sipila, Stephanie

    2014-01-01

    The Asteroid Redirect Crewed Mission (ARCM) requires a Launch/Entry/Abort (LEA) suit capability and short duration Extra Vehicular Activity (EVA) capability from the Orion spacecraft. For this mission, the pressure garment selected for both functions is the Modified Advanced Crew Escape Suit (MACES) with EVA enhancements and the life support option that was selected is the Exploration Portable Life Support System (PLSS) currently under development for Advanced Exploration Systems (AES). The proposed architecture meets the ARCM constraints, but much more work is required to determine the details of the suit upgrades, the integration with the PLSS, and the tools and equipment necessary to accomplish the mission. This work has continued over the last year to better define the operations and hardware maturation of these systems. EVA simulations were completed in the Neutral Buoyancy Lab (NBL) and interfacing options were prototyped and analyzed with testing planned for late 2014. This paper discusses the work done over the last year on the MACES enhancements, the use of tools while using the suit, and the integration of the PLSS with the MACES.

  15. DEALed : A tool suite for distributed real-time systems development

    NARCIS (Netherlands)

    Bolshakov, K.; Karpov, Y.; Sintotski, A.; Malyshkin, V.

    1999-01-01

    DEALed is a tool suite for development of distributed systems using DEAL language. DEAL is being developed at Eindhoven University of Technology as a part of DEDOS project. Area of application of the DEALed is the development of the distributed real- time safety-critical control systems.

  16. UKRmol: a low-energy electron- and positron-molecule scattering suite

    Science.gov (United States)

    Carr, J. M.; Galiatsatos, P. G.; Gorfinkiel, J. D.; Harvey, A. G.; Lysaght, M. A.; Madden, D.; Mašín, Z.; Plummer, M.; Tennyson, J.; Varambhia, H. N.

    2012-03-01

    We describe the UK computational implementation of the R-matrix method for the treatment of electron and positron scattering from molecules. Recent developments in the UKRmol suite are detailed together with the collision processes it is enabling us to treat.

  17. Effectiveness comparison of partially executed t-way test suite based generated by existing strategies

    Science.gov (United States)

    Othman, Rozmie R.; Ahmad, Mohd Zamri Zahir; Ali, Mohd Shaiful Aziz Rashid; Zakaria, Hasneeza Liza; Rahman, Md. Mostafijur

    2015-05-01

    Consuming 40 to 50 percent of software development cost, software testing is one of the most resource consuming activities in software development lifecycle. To ensure an acceptable level of quality and reliability of a typical software product, it is desirable to test every possible combination of input data under various configurations. Due to combinatorial explosion problem, considering all exhaustive testing is practically impossible. Resource constraints, costing factors as well as strict time-to-market deadlines are amongst the main factors that inhibit such consideration. Earlier work suggests that sampling strategy (i.e. based on t-way parameter interaction or called as t-way testing) can be effective to reduce number of test cases without effecting the fault detection capability. However, for a very large system, even t-way strategy will produce a large test suite that need to be executed. In the end, only part of the planned test suite can be executed in order to meet the aforementioned constraints. Here, there is a need for test engineers to measure the effectiveness of partially executed test suite in order for them to assess the risk they have to take. Motivated by the abovementioned problem, this paper presents the effectiveness comparison of partially executed t-way test suite generated by existing strategies using tuples coverage method. Here, test engineers can predict the effectiveness of the testing process if only part of the original test cases is executed.

  18. Getting Started With Oracle SOA Suite 11g R1 - A Hands-On Tutorial

    CERN Document Server

    Buelow, Heidi; Palvankar, Prasen

    2009-01-01

    This fully illustrated step-by-step tutorial is based on proven training material that has been highly praised by hundreds of developers in product training courses given as part of the SOA Suite 11g rollout. You will learn how to build a services-oriente

  19. An Effective Strategy to Build Up a Balanced Test Suite for Spectrum-Based Fault Localization

    Directory of Open Access Journals (Sweden)

    Ning Li

    2016-01-01

    Full Text Available During past decades, many automated software faults diagnosis techniques including Spectrum-Based Fault Localization (SBFL have been proposed to improve the efficiency of software debugging activity. In the field of SBFL, suspiciousness calculation is closely related to the number of failed and passed test cases. Studies have shown that the ratio of the number of failed and passed test case has more significant impact on the accuracy of SBFL than the total number of test cases, and a balanced test suite is more beneficial to improving the accuracy of SBFL. Based on theoretical analysis, we proposed an PNF (Passed test cases, Not execute Faulty statement strategy to reduce test suite and build up a more balanced one for SBFL, which can be used in regression testing. We evaluated the strategy making experiments using the Siemens program and Space program. Experiments indicated that our PNF strategy can be used to construct a new test suite effectively. Compared with the original test suite, the new one has smaller size (average 90% test case was reduced in experiments and more balanced ratio of failed test cases to passed test cases, while it has the same statement coverage and fault localization accuracy.

  20. Data Analysis and Graphing in an Introductory Physics Laboratory: Spreadsheet versus Statistics Suite

    Science.gov (United States)

    Peterlin, Primoz

    2010-01-01

    Two methods of data analysis are compared: spreadsheet software and a statistics software suite. Their use is compared analysing data collected in three selected experiments taken from an introductory physics laboratory, which include a linear dependence, a nonlinear dependence and a histogram. The merits of each method are compared. (Contains 7…

  1. T3i: A Tool for Generating and Querying Test Suites for Java

    NARCIS (Netherlands)

    Prasetya, I.S.W.B.

    2015-01-01

    T3i is an automated unit-testing tool to test Java classes. To expose interactions T3i generates test-cases in the form of sequences of calls to the methods of the target class. What separates it from other testing tools is that it treats test suites as first class objects and allows users to e.g.

  2. The VI-Suite: a set of environmental analysis tools with geospatial data applications

    NARCIS (Netherlands)

    Southall, Ryan; Biljecki, F.

    2017-01-01

    Background: The VI-Suite is a free and open-source addon for the 3D content creation application Blender, developed primarily as a tool for the contextual and performative analysis of buildings. Its functionality has grown from simple, static lighting analysis to fully parametric lighting,

  3. The pressurized suit and some considerations leading to the development of physiologically safe working conditions

    International Nuclear Information System (INIS)

    Evans, J.C.

    1980-01-01

    The safe working environment of men wearing pressurized suits in the U.K.A.E.A. is based on the results of two experimental programmes, the first investigated the respiratory hazards, the second the assessment and control of the level of thermal stress. Both are described. (author)

  4. Malpractice suits in chest radiology: an evaluation of the histories of 8265 radiologists.

    Science.gov (United States)

    Baker, Stephen R; Patel, Ronak H; Yang, Lily; Lelkes, Valdis M; Castro, Alejandro

    2013-11-01

    The aim of this study was to present rates of claims, causes of error, percentage of cases resulting in a judgment, and average payments made by radiologists in chest-related malpractice cases in a survey of 8265 radiologists. The malpractice histories of 8265 radiologists were evaluated from the credentialing files of One-Call Medical Inc., a preferred provider organization for computed tomography/magnetic resonance imaging in workers' compensation cases. Of the 8265 radiologists, 2680 (32.4%) had at least 1 malpractice suit. Of those who were sued, the rate of claims was 55.1 per 1000 person years. The rate of thorax-related suits was 6.6 claims per 1000 radiology practice years (95% confidence interval, 6.0-7.2). There were 496 suits encompassing 48 different causes. Errors in diagnosis comprised 78.0% of the causes. Failure to diagnose lung cancer was by far the most frequent diagnostic error, representing 211 cases or 42.5%. Of the 496 cases, an outcome was known in 417. Sixty-one percent of these were settled in favor of the plaintiff, with a mean payment of $277,230 (95% confidence interval, 226,967-338,614). Errors in diagnosis, and among them failure to diagnose lung cancer, were by far the most common reasons for initiating a malpractice suit against radiologists related to the thorax and its contents.

  5. Community Intercomparison Suite (CIS) v1.4.0: A tool for intercomparing models and observations

    NARCIS (Netherlands)

    Watson-Parris, Duncan; Schutgens, Nick; Cook, Nicholas; Kipling, Zak; Kershaw, Philip; Gryspeerdt, Edward; Lawrence, Bryan; Stier, Philip

    2016-01-01

    The Community Intercomparison Suite (CIS) is an easy-to-use command-line tool which has been developed to allow the straightforward intercomparison of remote sensing, in-situ and model data. While there are a number of tools available for working with climate model data, the large diversity of

  6. Data analysis and graphing in an introductory physics laboratory: spreadsheet versus statistics suite

    International Nuclear Information System (INIS)

    Peterlin, Primoz

    2010-01-01

    Two methods of data analysis are compared: spreadsheet software and a statistics software suite. Their use is compared analysing data collected in three selected experiments taken from an introductory physics laboratory, which include a linear dependence, a nonlinear dependence and a histogram. The merits of each method are compared.

  7. The IMAGE model suite used for the OECD Environmental Outlook to 2050

    Energy Technology Data Exchange (ETDEWEB)

    Kram, T.; Stehfest, E.

    2012-03-15

    In the Environmental Outlook to 2050 from the Organisation for Economic Co-operation and Development (OECD) a number of scenarios and projection are used which are calculated with the IMAGE model suite. This document describes the models and modules used and their interconnections.

  8. TypingSuite: Integrated Software for Presenting Stimuli, and Collecting and Analyzing Typing Data

    Science.gov (United States)

    Mazerolle, Erin L.; Marchand, Yannick

    2015-01-01

    Research into typing patterns has broad applications in both psycholinguistics and biometrics (i.e., improving security of computer access via each user's unique typing patterns). We present a new software package, TypingSuite, which can be used for presenting visual and auditory stimuli, collecting typing data, and summarizing and analyzing the…

  9. 78 FR 18979 - Proposed Settlement Agreement, Clean Air Act Citizen Suit

    Science.gov (United States)

    2013-03-28

    .... Jackson, Case No. 12- 1096 (D.D.C.) (``LEAN v. Jackson''). Plaintiffs filed this suit to compel the... INFORMATION CONTACT: Melina Williams, Air and Radiation Law Office (2344A), Office of General Counsel, U.S... Amended Complaint with prejudice in LEAN v. Jackson. In addition, the proposed settlement agreement...

  10. Vibrotactile perception assessment for a haptic interface on an antigravity suit.

    Science.gov (United States)

    Ko, Sang Min; Lee, Kwangil; Kim, Daeho; Ji, Yong Gu

    2017-01-01

    Haptic technology is used in various fields to transmit information to the user with or without visual and auditory cues. This study aimed to provide preliminary data for use in developing a haptic interface for an antigravity (anti-G) suit. With the structural characteristics of the anti-G suit in mind, we determined five areas on the body (lower back, outer thighs, inner thighs, outer calves, and inner calves) on which to install ten bar-type eccentric rotating mass (ERM) motors as vibration actuators. To determine the design factors of the haptic anti-G suit, we conducted three experiments to find the absolute threshold, moderate intensity, and subjective assessments of vibrotactile stimuli. Twenty-six fighter pilots participated in the experiments, which were conducted in a fixed-based flight simulator. From the results of our study, we recommend 1) absolute thresholds of ∼11.98-15.84 Hz and 102.01-104.06 dB, 2) moderate intensities of 74.36 Hz and 126.98 dB for the lower back and 58.65 Hz and 122.37 dB for either side of the thighs and calves, and 3) subjective assessments of vibrotactile stimuli (displeasure, easy to perceive, and level of comfort). The results of this study will be useful for the design of a haptic anti-G suit. Copyright © 2016 Elsevier Ltd. All rights reserved.

  11. Recommendations concerning models and parameters best suited to breeder reactor environmental radiological assessments

    International Nuclear Information System (INIS)

    Miller, C.W.; Baes, C.F. III; Dunning, D.E. Jr.

    1980-05-01

    Recommendations are presented concerning the models and parameters best suited for assessing the impact of radionuclide releases to the environment by breeder reactor facilities. These recommendations are based on the model and parameter evaluations performed during this project to date. Seven different areas are covered in separate sections

  12. 46 CFR 160.171-17 - Approval testing for adult size immersion suit.

    Science.gov (United States)

    2010-10-01

    ... test described in § 160.171-17(d). (c) Mobility and flotation tests. The mobility and flotation... suit at the beginning of the period of flotation by more than 200 grams. (11) Impact. While wearing a... is required for this test: (i) A sealed copper or aluminum can that has at least two parallel flat...

  13. MRI-guided stereotactic neurosurgical procedures in a diagnostic MRI suite: Background and safe practice recommendations.

    Science.gov (United States)

    Larson, Paul S; Willie, Jon T; Vadivelu, Sudhakar; Azmi-Ghadimi, Hooman; Nichols, Amy; Fauerbach, Loretta Litz; Johnson, Helen Boehm; Graham, Denise

    2017-07-01

    The development of navigation technology facilitating MRI-guided stereotactic neurosurgery has enabled neurosurgeons to perform a variety of procedures ranging from deep brain stimulation to laser ablation entirely within an intraoperative or diagnostic MRI suite while having real-time visualization of brain anatomy. Prior to this technology, some of these procedures required multisite workflow patterns that presented significant risk to the patient during transport. For those facilities with access to this technology, safe practice guidelines exist only for procedures performed within an intraoperative MRI. There are currently no safe practice guidelines or parameters available for facilities looking to integrate this technology into practice in conventional MRI suites. Performing neurosurgical procedures in a diagnostic MRI suite does require precautionary measures. The relative novelty of technology and workflows for direct MRI-guided procedures requires consideration of safe practice recommendations, including those pertaining to infection control and magnet safety issues. This article proposes a framework of safe practice recommendations designed for assessing readiness and optimization of MRI-guided neurosurgical interventions in the diagnostic MRI suite in an effort to mitigate patient risk. The framework is based on existing clinical evidence, recommendations, and guidelines related to infection control and prevention, health care-associated infections, and magnet safety, as well as the clinical and practical experience of neurosurgeons utilizing this technology. © 2017 American Society for Healthcare Risk Management of the American Hospital Association.

  14. 78 FR 43200 - Proposed Settlement Agreement, Clean Air Act Citizen Suit

    Science.gov (United States)

    2013-07-19

    ... Social Responsibility--Los Angeles v. EPA, No. 12-56175, upon receipt of written notice from EPA that the... ENVIRONMENTAL PROTECTION AGENCY [EPA-HQ-OGC-2013-0484; FRL-9835-6] Proposed Settlement Agreement, Clean Air Act Citizen Suit AGENCY: Environmental Protection Agency (EPA). ACTION: Notice of proposed...

  15. Optimizing the physical ergonomics indices for the use of partial pressure suits.

    Science.gov (United States)

    Ding, Li; Li, Xianxue; Hedge, Alan; Hu, Huimin; Feathers, David; Qin, Zhifeng; Xiao, Huajun; Xue, Lihao; Zhou, Qianxiang

    2015-03-01

    This study developed an ergonomic evaluation system for the design of high-altitude partial pressure suits (PPSs). A total of twenty-one Chinese males participated in the experiment which tested three types of ergonomics indices (manipulative mission, operational reach and operational strength) were studied using a three-dimensional video-based motion capture system, a target-pointing board, a hand dynamometer, and a step-tread apparatus. In total, 36 ergonomics indices were evaluated and optimized using regression and fitting analysis. Some indices that were found to be linearly related and redundant were removed from the study. An optimal ergonomics index system was established that can be used to conveniently and quickly evaluate the performance of different pressurized/non-pressurized suit designs. The resulting ergonomics index system will provide a theoretical basis and practical guidance for mission planners, suit designers and engineers to design equipment for human use, and to aid in assessing partial pressure suits. Copyright © 2014 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  16. Get the lead out: Wildlife advocates file suit to replace toxic ammo ...

    African Journals Online (AJOL)

    2007-03-02

    Mar 2, 2007 ... Get the lead out: Wildlife advocates file suit to replace toxic ammo with safer alternatives. Simple, practical ... who eats game, which often contains shot or small metal fragments. Lead is an extremely toxic ... eating meat from animals taken with lead ammunition often have unhealthy lead levels in their own ...

  17. Effect of fastskin suits on performance, drag, and energy cost of swimming.

    Science.gov (United States)

    Chatard, Jean-Claude; Wilson, Barry

    2008-06-01

    To investigate the effect of fastskin suits on 25- to 800-m performances, drag, and energy cost of swimming. The performances, stroke rate and distance per stroke, were measured for 14 competitive swimmers in a 25-m pool, when wearing a normal suit (N) and when wearing a full-body suit (FB) or a waist-to-ankle suit (L). Passive drag, oxygen uptake, blood lactate, and the perceived exertion were measured in a flume. There was a 3.2% +/- 2.4% performance benefit for all subjects over the six distances covered at maximal speed wearing FB and L when compared with N. When wearing L, the gain was significantly lower (1.8% +/- 2.5%, P energy cost of swimming was significantly reduced when wearing FB and L by 4.5% +/- 5.4% and 5.5% +/- 3.1%, respectively (P energy cost of submaximal swimming and an increased distance per stroke, at the same stroke rates, and reduced freestyle performance time.

  18. Analisa Pengaruh Komunikasi Internal, Intrinsic Rewards Dan Recognition Terhadap Employee Engagement Di Surabaya Suite Hotel

    OpenAIRE

    Lorensia, Ines Margaretha; Ngo, Diana Kartika; Widjaja, Debora

    2015-01-01

    Penelitian ini dilakukan untuk menganalisa pengaruh komunikasi internal, intrinsic rewards, dan recognition terhadap employee engagementdi SurabayaSuiteHotel. Employee engagement penting untuk meningkatkan kinerja karyawan demi keberhasilan organisasi. Komunikasi internal adalah proses pertukaran informasi dalam internal organisasi. Intrinsic rewards adalah kepuasan pribadi dari pekerjaan itu sendiri. Dan recognition adalah pengakuan yang diberikan atas kinerja karyawan. Teknik analisa yang d...

  19. Rimsky-Korsakov: Symphony N2 (Symphonic Suite), Op. 9 / John Warrack

    Index Scriptorium Estoniae

    Warrack, John

    1990-01-01

    Uuest heliplaadist "Rimsky-Korsakov: Symphony N2 (Symphonic Suite), Op. 9, "Antar" Russian Easter Festival Overture, Op.36. Philharmonia Orchestra, Evgeni Svetlanov" Hyperion KA 66399. CDA 66399. Teise sümfoonia esitust võrreldud Neeme Järvi plaadistusega

  20. Nielsen: Aladdin-Suite, FS89. Maskarade-Overture / Robert Layton

    Index Scriptorium Estoniae

    Layton, Robert

    1996-01-01

    Uuest heliplaadist "Nielsen: Aladdin-Suite, FS89. Maskarade-Overture, Prelude, Act 2. The Cockerels' Dance. Rhapsody Overture: An imaginary journey to the Faroe Islands, FS123. Helios Overture, FS32. Saga-Drom, FS46. Pan and Syrinx, FS87. Gothenburg Symphony Orchestra, Neeme Järvi" DG 447 757-2GH (72 minutes: DDD)