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Sample records for profile lp sampled

  1. Sampling with removal in LP-type problems

    Directory of Open Access Journals (Sweden)

    Bernd Gärtner

    2015-02-01

    Full Text Available Random sampling is an important tool in optimization subject to finitely or infinitely many constraints. Here we are interested in obtaining solutions of low cost that violate only few constraints. Under convexity or similar favorable conditions, and assuming fixed dimension, one can indeed derive combinatorial bounds on the expected number (or probability mass of constraints violated by the optimal solution subject to a (small random sample of  constraints. The cost of the sample solution, however, cannot be bounded combinatorially.Suppose that after picking the sample, we remove $k$ constraints from it in such a way that the best improvement of objective function is achieved. Can we still guarantee (good bounds on the number or mass of violated constraints?  This question was asked and  partially answered in the framework of chance-constrained optimization.Here we study the question in the completely combinatorial setting LP-type problems, which allows a unified treatment of many different optimization problems. Given a nondegenerate LP-type problem of combinatorial dimension $\\delta$, we consider sampling with subsequent removal of the $k$ constraints that lead to the best improvement of objective function. We show that this induces an LP-type problem of combinatorial dimension $O(\\delta^{k+1}$, and that this bound is tight in the worst case. It follows that through this kind of removal, the expected number of violated constraints blows up by a factor of at most $O(\\delta^{k}$; for relevant sample sizes, however, a matching lower bound for the blowup factor is not implied. At the same time, our results show that further improvements require new tools that go beyond combinatorial dimension.

  2. OMPS-NPP L2 LP Aerosol Extinction Vertical Profile swath daily 3slit V1 (OMPS_NPP_LP_L2_AER675_DAILY) at GES DISC

    Data.gov (United States)

    National Aeronautics and Space Administration — The OMPS-NPP L2 LP Aerosol Extinction Vertical Profile swath daily 3slit collection contains the retrieved aerosol extinction coefficients at 675 nm (AER675)...

  3. SCIAMACHY stratospheric aerosol extinction profile retrieval using the OMPS/LP algorithm

    Directory of Open Access Journals (Sweden)

    G. Taha

    2011-03-01

    Full Text Available The Ozone Mapper and Profiler Suite, Limp Profiler (OMPS/LP algorithm is used to retrieve ozone concentration and aerosol extinction profiles using a series of 120 SCIAMACHY limb measurements collocated with SAGE II solar occultation events. The primary goal of the study is to ascertain the capability of the OMPS/LP retrieval algorithm to accurately retrieve the vertical distribution of stratospheric aerosol extinction coefficient so as to better account for aerosol effects in the ozone profiling retrieval process. Using simulated radiances, we show that the aerosol extinction coefficient can be retrieved from limb scatter measurements within 5% and a standard deviation better than 15%, which is more than sufficient to improve the OMPS/LP ozone products to be used as Environmental Data Records. We also illustrate the ability of SCIAMACHY limb measurements to retrieve stratospheric aerosol extinction profiles with accuracy comparable to other instruments. The retrieved aerosol extinction profiles agree with collocated SAGE II measurements on average to within 25%, with a standard deviation of 35%.

  4. The Lp Lp Lp-curvature images of convex bodies and Lp Lp Lp ...

    Indian Academy of Sciences (India)

    Abstract. Associated with the Lp-curvature image defined by Lutwak, some inequali- ties for extended mixed p-affine surface areas of convex bodies and the support functions of Lp-projection bodies are established. As a natural extension of a result due to Lutwak, an Lp-type affine isoperimetric inequality, whose special ...

  5. [Differences in Measured Values among Homogenous Assay Reagents of LDL-C in LP-X Positive Serum Samples].

    Science.gov (United States)

    Abe, Misako; Kurosawa, Hideo; Sato, Ryo; Ito, Kumie; Tomono, Yoshiharu; Manita, Daisuke; Hirowatari, Yuji; Yoshida, Hiroshi

    2015-03-01

    The LDL-C level measures with homogeneous (direct) assays in almost of clinical laboratories. Several reports however showed differences in measured values among the assay reagents. We investigated the differences in LDL-C values among direct assays and Friedewald formula (F-f) in 58 LP-X positive serum samples from jaundice patients by comparing LDL-C values measured by anion-exchange chromatography (AEX-HPLC), largely comparable to ultracentrifugation method. Changes in LDL-C values during the treatment of 8 patients were also investigated. Direct assay reagents from Sekisui Medical (S-r), Denka-Seiken (D-r), Wako Chemical (W-r), and Kyowa Medics (K-r) were used for comparison. F-f, S-r, and D-r correlated with AEX-HPLC with r values 0.6. Two samples in which F-f values provided 500 mg/dL plus bias to AEX-HPLC (LDL-C value of 220 mg/dL) demonstrated increased levels of IDL-C before treatment. LDL-C values (S-r and D-r) of the 2 samples were relatively high and near to F-f data while LDL-C values (W-r and K-r) were relatively low and close to AEX-HPLC data. The jaundice treatment decreased LDL-C values (S-r and D-r) and converged to 220 mg/dL, indicating that S-r and D-r might react markedly to IDL. These changes were consistent with decreases in serum free cholesterol and phospholipid in support of LP-X. By contrast, W-r and K-r data showed upward tendency and also converged to 220 mg/dL. These results suggest that LDL-C direct assay reagents would be classified into 2 groups with respect to the reagent reactivity to LP-X.

  6. LP L RSS LINE OF SIGHT ACCELERATION PROFILES V1.0

    Data.gov (United States)

    National Aeronautics and Space Administration — Line of Sight Acceleration Profile Data Records (LOSAPDR) consist of data from Doppler tracking of the orbiting spacecraft. The relative motion of the spacecraft and...

  7. LP-gas marketers nationwide reveal hardships encountered during crisis

    Energy Technology Data Exchange (ETDEWEB)

    1990-04-01

    The authors report on a survey of 150 randomly-selected LP-gas marketers nationwide. Replies were solicited from every major geographical region. The purpose of the survey was to obtain a comprehensive sampling of information on local price increases, shipping difficulties, relations with customers, and strategies for the future. This paper presents a profile that sums up the effects of the propane emergency on marketers and customers.

  8. Lp Lp Lp-mixed intersection bodies and star duality

    Indian Academy of Sciences (India)

    Lutwak introduced mixed projection bodies and derived their fundamental inequalities. In 1999, Moszynska [28] introduced the notion of star dual of star body. Recently,. Haberl and Ludwig [16] introduced Lp-intersection bodies. Following Haberl, Ludwig and Moszynska, the paper introduces a new concept of q-dual mixed ...

  9. Using MERRA-2 analysis fields to simulate limb scattered radiance profiles for inhomogeneous atmospheric lines of sight: Preparation for data assimilation of OMPS LP radiances through 2D single-scattering GSLS radiative transfer model development

    Science.gov (United States)

    Loughman, R. P.; Bhartia, P. K.; Moy, L.; Kramarova, N. A.; Wargan, K.

    2016-12-01

    Many remote sensing techniques used to monitor the Earth's upper atmosphere fall into the broad category of "limb viewing" (LV) measurements, which includes any method for which the line of sight (LOS) fails to intersect the surface. Occultation, limb emission and limb scattering (LS) measurements are all LV methods that offer strong sensitivity to changes in the atmosphere near the tangent point of the LOS, due to the enhanced geometric path through the tangent layer (where the concentration also typically peaks, for most atmospheric species). But many of the retrieval algorithms used to interpret LV measurements assume that the atmosphere consists of "spherical shells", in which the atmospheric properties vary only with altitude (creating a 1D atmosphere). This assumption simplifies the analysis, but at the possible price of misinterpreting measurements made in the real atmosphere. In this presentation, we focus on the problem of LOS inhomogeneity for LS measurements made by the OMPS Limb Profiler (LP) instrument during the 2015 ozone hole period. The GSLS radiative transfer model (RTM) used in the default OMPS LP algorithms assumes a spherical-shell atmosphere defined at levels spaced 1 km apart, with extinction coefficients assumed to vary linearly with height between levels. Several recent improvements enable an updated single-scattering version of the GSLS RTM to ingest 3D MERRA-2 analysis fields (including temperature, pressure, and ozone concentration) when creating the model atmosphere, by introducing flexible altitude grids, flexible atmospheric specification along the LOS, and improved treatment of the radiative transfer within each atmospheric layer. As a result, the effect of LOS inhomogeneity on the current (1D) OMPS LP retrieval algorithm can now be studied theoretically, using realistic 3D atmospheric profiles. This work also represents a step towards enabling OMPS LP data to be ingested as part of future data assimilation efforts.

  10. Determination of DNA profiling of siwak and toothbrush samples ...

    African Journals Online (AJOL)

    Background: DNA profiling is an integral part of forensic work. Enough and good samples for DNA quantification and profiling are mandatory. Aim of the study: To quantify and profile DNA from siwak and toothbrushes and study the effect of time on this process. Methodology: The present study included DNA profiling from ...

  11. Profile Analysis of the Universal Nonverbal Intelligence Test Standardization Sample.

    Science.gov (United States)

    Wilhoit, Brian E.; McCallum, R. Steve

    2002-01-01

    A normative typology was developed and applied using multivariate profile analysis of subtest scores of the Universal Nonverbal Intelligence Test (UNIT) standardization sample. The results yielded a seven-profile cluster solution for the Extended Battery, and a six-profile cluster solution for the Standard Battery. Additionally, the results lend…

  12. CORN, LP Goldfield Approval

    Science.gov (United States)

    This November 19, 2015 letter from EPA approves the petition from CORN, LP, Goldfield facility, regarding non-grandfathered ethanol produced through a dry mill process, qualifying under the Clean Air Act for renewable fuel (D-code 6) RINs under the RFS pro

  13. Isometric multipliers of Lp(G, X) Lp(G, X) Lp(G, X)

    Indian Academy of Sciences (India)

    R. Narasimhan (Krishtel eMaging) 1461 1996 Oct 15 13:05:22

    Abstract. Let G be a locally compact group with a fixed right Haar measure and X a separable Banach space. Let Lp(G, X) be the space of X-valued measurable functions whose norm-functions are in the usual Lp . A left multiplier of Lp(G, X) is a bounded linear operator on Lp(G, X) which commutes with all left translations.

  14. Toxic Metals Profiles in Hair Samples from Street Roaming Animals ...

    African Journals Online (AJOL)

    Assessment of toxic metals profiles in hair samples from street roaming animals in Yelwa-Yauri town, North Western, Nigeria was carried out. Hair samples for the analyses were collected from 108 animal (54 sheep and 54 goats) roaming the street of Yelwa-Yauri town and 45 samples from animals in the neighboring ...

  15. Apolipoprotein AI- and AI:AII-containing lipoproteins in white men and women of the HERITAGE Family study: Associations with metabolic risk profile variables.

    Science.gov (United States)

    Couillard, Charles; Bergeron, Jean; Després, Jean-Pierre; Gagnon, Jacques; Rankinen, Tuomo; Leon, Arthur S; Rao, D C; Skinner, James S; Wilmore, Jack H; Bouchard, Claude

    2003-12-01

    It is now well established that an increased high-density lipoprotein (HDL) cholesterol level, especially in the HDL(2) subfraction, is associated with a reduced risk of coronary heart disease (CHD). However, little is known about the associations between the apolipoprotein (apo) composition of HDL and CHD metabolic risk factors. In the present study, we examined the gender differences in plasma concentration of HDL containing apo AI only (LpAI) versus both apoAI and apoAII (LpAI/AII), and also compared their associations with body composition, adipose tissue (AT) distribution, and metabolic risk profile variables. For that purpose, we measured fasting plasma lipoprotein-lipid levels including LpAI and LpAI/AII concentrations in a sample of 215 men and 174 women, all Caucasians, of the HERITAGE Family Study. All subjects underwent anthropometric, body fatness (underwater weighing) and abdominal AT accumulation (computed tomography) measurements. We found that, women had higher LpAI and lower LpAI/AII concentrations compared with men. Whereas in women, LpAI levels were correlated to body fat mass and waist circumference, no association between body composition, fat distribution, and LpAI concentrations was noted in men. Increased LpAI concentrations were associated with higher HDL(2) cholesterol levels in both men and women. Overall, elevated LpAI and LpAI/AII concentrations showed contrasting associations with metabolic risk profile variables as high LpAI, but not LpAI/AII concentrations were associated with a more favorable metabolic risk profile. We also found that high HDL cholesterol appeared to be more closely related to a better metabolic risk profile than high LpAI in both genders. Our results suggest that LpAI and HDL cholesterol levels are good correlates of the metabolic profile, but that HDL cholesterol concentrations could still represent a better index in CHD risk assessment.

  16. Lp(a-cholesterol is associated with HDL-cholesterol in overweight and obese African American children and is not an independent risk factor for CVD

    Directory of Open Access Journals (Sweden)

    Sharma Sushma

    2012-01-01

    Full Text Available Abstract Background The role of Lipoprotein (a cholesterol {Lp(a-C}as an additional and/or independent risk factor for cardiovascular disease (CVD is not clear. We evaluated the associations between Lp(a-C and other CVD risk factors including plasma lipoprotein concentrations and body fatness in overweight and obese African American children. Methods A cross-sectional analysis was carried out using data from a sample of 121 African American children aged 9-11 years with Body Mass Index (BMI's greater than the 85th percentile. Body height, weight and waist circumference (WC were measured. Fasting plasma concentrations of Lp(a-C, Total cholesterol (TC, High density lipoprotein cholesterol (HDL-C, Very low density lipoprotein cholesterol (VLDL-C, Intermediate density lipoprotein cholesterol (IDL-C, Low density lipoprotein cholesterol (LDL-C, and Triacylglycerides (TAG were analyzed using the vertical auto profile (VAP cholesterol method. Results After adjusting for child age, gender, and pubertal status, Lp(a-C was positively associated with both HDL-C and TC, and negatively associated with VLDL-C and TAG. Including BMIz and WC as additional covariates did not alter the direction of the relationships between Lp(a-C and the other lipoproteins. Finally, after adjusting for the other plasma lipoproteins, Lp(a-C remained strongly associated with HDL-C, whereas the associations of Lp(a-C with the other lipoproteins were not significant when HDL-C was simultaneously included in the regression models. Conclusions Lp(a-C was positively associated with HDL-C and this association is not influenced by other lipoprotein subclasses or by the degree of obesity. We conclude that Lp(a cholesterol is not an independent risk factor for CVD in African American children.

  17. Deep clonal profiling of formalin fixed paraffin embedded clinical samples.

    Directory of Open Access Journals (Sweden)

    Tara Holley

    Full Text Available Formalin fixed paraffin embedded (FFPE tissues are a vast resource of annotated clinical samples. As such, they represent highly desirable and informative materials for the application of high definition genomics for improved patient management and to advance the development of personalized therapeutics. However, a limitation of FFPE tissues is the variable quality of DNA extracted for analyses. Furthermore, admixtures of non-tumor and polyclonal neoplastic cell populations limit the number of biopsies that can be studied and make it difficult to define cancer genomes in patient samples. To exploit these valuable tissues we applied flow cytometry-based methods to isolate pure populations of tumor cell nuclei from FFPE tissues and developed a methodology compatible with oligonucleotide array CGH and whole exome sequencing analyses. These were used to profile a variety of tumors (breast, brain, bladder, ovarian and pancreas including the genomes and exomes of matching fresh frozen and FFPE pancreatic adenocarcinoma samples.

  18. Mineral Profile of Children's Fast Food Menu Samples.

    Science.gov (United States)

    Cervera, Maria Luisa; de la Guardia, Miguel; Ruiz-de-Cenzano, Manuela; Rochina-Marco, Arancha; López-Salazar, Óscar

    2017-05-26

    Children's fast food menus, including hamburgers served with french fries, dessert, and a soft drink, were analyzed to obtain the mineral profile of trace elements. The developed analytical methodology involved sample digestion under pressure inside a microwave oven with a mixture of HNO₃ and H₂O₂ and inductively coupled plasma-optical emission spectrometry. The method was validated by carrying out the analysis of certified reference materials (NIST 1570a spinach leaves, NCS ZC73016 chicken, and NIST 1568a rice flour) and using recovery experiments. Repeatability was verified by analyzing replicate samples. Twenty-six elements were studied, 12 of which-aluminum, barium, calcium, copper, iron, potassium, lithium, magnesium, manganese, sodium, strontium, and zinc-were quantitatively determined. Results were compared with other studies of fast food and children's menus published in the literature, and the nutritional value of samples was assessed with dietary intake guidelines.

  19. Headspace profiling of cocaine samples for intelligence purposes.

    Science.gov (United States)

    Dujourdy, Laurence; Besacier, Fabrice

    2008-08-06

    A method for determination of residual solvents in illicit hydrochloride cocaine samples using static headspace-gas chromatography (HS-GC) associated with a storage computerized procedure is described for the profiling and comparison of seizures. The system involves a gas chromatographic separation of 18 occluded solvents followed by fully automatic data analysis and transfer to a PHP/MySQL database. First, a fractional factorial design was used to evaluate the main effects of some critical method parameters (salt choice, vial agitation intensity, oven temperature, pressurization and loop equilibration) on the results with a minimum of experiments. The method was then validated for tactical intelligence purposes (batch comparison) via several studies: selection of solvents and mathematical comparison tool, reproducibility and "cutting" influence studies. The decision threshold to determine the similarity of two samples was set and false positives and negatives evaluated. Finally, application of the method to distinguish geographical origins is discussed.

  20. Online lektiehjælp

    DEFF Research Database (Denmark)

    Hansen, Jens Jørgen; Remvig, Kirstin

    2016-01-01

    vejledningsdidaktik, herunder metoder, modeller og materialer for lektievejledning i regi af Lektier Online. Lektier Online er en organisation ved Statsbiblioteket i Århus, der tilbyder en online lektiecafe, hvor bl.a. gymnasie-elever kan få hjælp af frivillige universitetsstuderende. Dette didaktiske de-sign er et...

  1. FANTOM5 CAGE profiles of human and mouse samples

    KAUST Repository

    Noguchi, Shuhei

    2017-08-29

    In the FANTOM5 project, transcription initiation events across the human and mouse genomes were mapped at a single base-pair resolution and their frequencies were monitored by CAGE (Cap Analysis of Gene Expression) coupled with single-molecule sequencing. Approximately three thousands of samples, consisting of a variety of primary cells, tissues, cell lines, and time series samples during cell activation and development, were subjected to a uniform pipeline of CAGE data production. The analysis pipeline started by measuring RNA extracts to assess their quality, and continued to CAGE library production by using a robotic or a manual workflow, single molecule sequencing, and computational processing to generate frequencies of transcription initiation. Resulting data represents the consequence of transcriptional regulation in each analyzed state of mammalian cells. Non-overlapping peaks over the CAGE profiles, approximately 200,000 and 150,000 peaks for the human and mouse genomes, were identified and annotated to provide precise location of known promoters as well as novel ones, and to quantify their activities.

  2. Determination of DNA profiling of siwak and toothbrush samples ...

    African Journals Online (AJOL)

    Nagy Alfadaly

    2016-06-01

    Jun 1, 2016 ... Abstract Background: DNA profiling is an integral part of forensic work. Enough and good sam- ples for DNA quantification and profiling are mandatory. Aim of the study: To quantify and profile DNA from siwak and toothbrushes and study the effect of time on this process. Methodology: The present study ...

  3. Expression and Genomic Profiling of Minute Breast Cancer Samples

    Science.gov (United States)

    2006-07-01

    Alteration of gene expression profiles of peripheral mononuclear blood cells by tobacco smoke : implications for periodontal diseases . Oral...potentially revolutionize (2) the existing cancer staging system and the management of early disease . Microarray- based gene expression profiling and...2002) Understanding disease cell by cell. Science, 296, 1329-1330. 15. Emmert-Buck, M.R., Bonner, R.F., Smith, P.D., Chuaqui, R.F., Zhuang, Z

  4. Temperament profiles of Sasang typology in a child clinical sample

    Directory of Open Access Journals (Sweden)

    Soo Jin Lee

    2012-12-01

    Conclusion: These results demonstrated distinct temperament traits associated with traditional Korean Sasang types in children using an objective biopsychological personality inventory. With further investigation into the biopsychological profiles of the children, the longitudinal stability of the Sasang typology can be examined.

  5. Profiling critical cancer gene mutations in clinical tumor samples.

    Directory of Open Access Journals (Sweden)

    Laura E MacConaill

    2009-11-01

    Full Text Available Detection of critical cancer gene mutations in clinical tumor specimens may predict patient outcomes and inform treatment options; however, high-throughput mutation profiling remains underdeveloped as a diagnostic approach. We report the implementation of a genotyping and validation algorithm that enables robust tumor mutation profiling in the clinical setting.We developed and implemented an optimized mutation profiling platform ("OncoMap" to interrogate approximately 400 mutations in 33 known oncogenes and tumor suppressors, many of which are known to predict response or resistance to targeted therapies. The performance of OncoMap was analyzed using DNA derived from both frozen and FFPE clinical material in a diverse set of cancer types. A subsequent in-depth analysis was conducted on histologically and clinically annotated pediatric gliomas. The sensitivity and specificity of OncoMap were 93.8% and 100% in fresh frozen tissue; and 89.3% and 99.4% in FFPE-derived DNA. We detected known mutations at the expected frequencies in common cancers, as well as novel mutations in adult and pediatric cancers that are likely to predict heightened response or resistance to existing or developmental cancer therapies. OncoMap profiles also support a new molecular stratification of pediatric low-grade gliomas based on BRAF mutations that may have immediate clinical impact.Our results demonstrate the clinical feasibility of high-throughput mutation profiling to query a large panel of "actionable" cancer gene mutations. In the future, this type of approach may be incorporated into both cancer epidemiologic studies and clinical decision making to specify the use of many targeted anticancer agents.

  6. Chemical profiling of six samples of Brazilian propolis

    Directory of Open Access Journals (Sweden)

    Caroline C. Fernandes-Silva

    2013-01-01

    Full Text Available Six samples of Brazilian propolis from Minas Gerais and Paraná states were analyzed to identify the constituents (GC/MS and HPLC/MS and to determine their contents (HPLC and external standardization. All samples contained characteristic constituents of green propolis, but the samples from Minas Gerais had higher contents of prenylated phenylpropanoids and caffeoylquinic acids. Kaempferide and two other flavonoids were among the major constituents of the samples from Minas Gerais. Luteolin 5-O-methyl ether was detected only in samples from Paraná. Baccharis dracunculifolia was a source of resins for all samples analyzed, but the samples from Paraná had more complex plant origin.

  7. Expression and Genomic Profiling of Minute Breast Cancer Samples. Addendum

    Science.gov (United States)

    2007-07-01

    Alteration of gene expression profiles of peripheral mononuclear blood cells by tobacco smoke : implications for periodontal diseases . Oral Microbiol...promises to refine (1) and potentially revolutionize (2) the existing cancer staging system and the management of early disease . Microarray- based...14. Rubin, M.A. (2002) Understanding disease cell by cell. Science, 296, 1329-1330. 12 13 15. Emmert-Buck, M.R., Bonner, R.F., Smith, P.D

  8. Sample storage conditions significantly influence faecal microbiome profiles.

    Science.gov (United States)

    Choo, Jocelyn M; Leong, Lex E X; Rogers, Geraint B

    2015-11-17

    Sequencing-based studies of the human faecal microbiota are increasingly common. Appropriate storage of sample material is essential to avoid the introduction of post-collection bias in microbial community composition. Rapid freezing to -80 °C is commonly considered to be best-practice. However, this is not feasible in many studies, particularly those involving sample collection in participants' homes. We determined the extent to which a range of stabilisation and storage strategies maintained the composition of faecal microbial community structure relative to freezing to -80 °C. Refrigeration at 4 °C, storage at ambient temperature, and the use of several common preservative buffers (RNAlater, OMNIgene.GUT, Tris-EDTA) were assessed relative to freezing. Following 72 hours of storage, faecal microbial composition was assessed by 16 S rRNA amplicon sequencing. Refrigeration was associated with no significant alteration in faecal microbiota diversity or composition. However, samples stored using other conditions showed substantial divergence compared to -80 °C control samples. Aside from refrigeration, the use of OMNIgene.GUT resulted in the least alteration, while the greatest change was seen in samples stored in Tris-EDTA buffer. The commercially available OMNIgene.GUT kit may provide an important alternative where refrigeration and cold chain transportation is not available.

  9. Low-profile self-sealing sample transfer flexure box.

    Science.gov (United States)

    Simon, Kevin; Porz, Lukas; Swamy, Tushar; Chiang, Yet-Ming; Slocum, Alexander

    2017-08-01

    A flexural bearing mechanism has enabled the development of a self-sealing box for protecting air sensitive samples during transfer between glove boxes, micro-machining equipment, and microscopy equipment. The simplicity and self-actuating feature of this design makes it applicable to many devices that operate under vacuum conditions. The models used to design the flexural mechanism are presented in detail. The device has been tested in a Zeiss Merlin GEMINI II scanning electron microscope with Li 3 PS 4 samples, showing effective isolation from air and corrosion prevention.

  10. FANTOM5 CAGE profiles of human and mouse samples

    NARCIS (Netherlands)

    Noguchi, Shuhei; Arakawa, Takahiro; Fukuda, Shiro; Furuno, Masaaki; Hasegawa, Akira; Hori, Fumi; Ishikawa-Kato, Sachi; Kaida, Kaoru; Kaiho, Ai; Kanamori-Katayama, Mutsumi; Kawashima, Tsugumi; Kojima, Miki; Kubosaki, Atsutaka; Manabe, Ri-ichiroh; Murata, Mitsuyoshi; Nagao-Sato, Sayaka; Nakazato, Kenichi; Ninomiya, Noriko; Nishiyori-Sueki, Hiromi; Noma, Shohei; Saijyo, Eri; Saka, Akiko; Sakai, Mizuho; Simon, Christophe; Suzuki, Naoko; Tagami, Michihira; Watanabe, Shoko; Yoshida, Shigehiro; Arner, Peter; Axton, Richard A.; Babina, Magda; Baillie, J. Kenneth; Barnett, Timothy C.; Beckhouse, Anthony G.; Blumenthal, Antje; Bodega, Beatrice; Bonetti, Alessandro; Briggs, James; Brombacher, Frank; Carlisle, Ailsa J.; Clevers, Hans C.; Davis, Carrie A.; Detmar, Michael; Dohi, Taeko; Edge, Albert S. B.; Edinger, Matthias; Ehrlund, Anna; Ekwall, Karl; Endoh, Mitsuhiro; Enomoto, Hideki; Eslami, Afsaneh; Fagiolini, Michela; Fairbairn, Lynsey; Farach-Carson, Mary C.; Faulkner, Geoffrey J.; Ferrai, Carmelo; Fisher, Malcolm E.; Forrester, Lesley M.; Fujita, Rie; Furusawa, Jun-ichi; Geijtenbeek, Teunis B.; Gingeras, Thomas; Goldowitz, Daniel; Guhl, Sven; Guler, Reto; Gustincich, Stefano; Ha, Thomas J.; Hamaguchi, Masahide; Hara, Mitsuko; Hasegawa, Yuki; Herlyn, Meenhard; Heutink, Peter; Hitchens, Kelly J.; Hume, David A.; Ikawa, Tomokatsu; Ishizu, Yuri; Kai, Chieko; Kawamoto, Hiroshi; Kawamura, Yuki I.; Kempfle, Judith S.; Kenna, Tony J.; Kere, Juha; Khachigian, Levon M.; Kitamura, Toshio; Klein, Sarah; Klinken, S. Peter; Knox, Alan J.; Kojima, Soichi; Koseki, Haruhiko; Koyasu, Shigeo; Lee, Weonju; Lennartsson, Andreas; Mackay-sim, Alan; Mejhert, Niklas; Mizuno, Yosuke; Morikawa, Hiromasa; Morimoto, Mitsuru; Moro, Kazuyo; Morris, Kelly J.; Motohashi, Hozumi; Mummery, Christine L.; Nakachi, Yutaka; Nakahara, Fumio; Nakamura, Toshiyuki; Nakamura, Yukio; Nozaki, Tadasuke; Ogishima, Soichi; Ohkura, Naganari; Ohno, Hiroshi; Ohshima, Mitsuhiro; Okada-Hatakeyama, Mariko; Okazaki, Yasushi; Orlando, Valerio; Ovchinnikov, Dmitry A.; Passier, Robert; Patrikakis, Margaret; Pombo, Ana; Pradhan-Bhatt, Swati; Qin, Xian-Yang; Rehli, Michael; Rizzu, Patrizia; Roy, Sugata; Sajantila, Antti; Sakaguchi, Shimon; Sato, Hiroki; Satoh, Hironori; Savvi, Suzana; Saxena, Alka; Schmidl, Christian; Schneider, Claudio; Schulze-Tanzil, Gundula G.; Schwegmann, Anita; Sheng, Guojun; Shin, Jay W.; Sugiyama, Daisuke; Sugiyama, Takaaki; Summers, Kim M.; Takahashi, Naoko; Takai, Jun; Tanaka, Hiroshi; Tatsukawa, Hideki; Tomoiu, Andru; Toyoda, Hiroo; van de Wetering, Marc; van den Berg, Linda M.; Verardo, Roberto; Vijayan, Dipti; Wells, Christine A.; Winteringham, Louise N.; Wolvetang, Ernst; Yamaguchi, Yoko; Yamamoto, Masayuki; Yanagi-Mizuochi, Chiyo; Yoneda, Misako; Yonekura, Yohei; Zhang, Peter G.; Zucchelli, Silvia; Abugessaisa, Imad; Arner, Erik; Harshbarger, Jayson; Kondo, Atsushi; Lassmann, Timo; Lizio, Marina; Sahin, Serkan; Sengstag, Thierry; Severin, Jessica; Shimoji, Hisashi; Suzuki, Masanori; Suzuki, Harukazu; Kawai, Jun; Kondo, Naoto; Itoh, Masayoshi; Daub, Carsten O.; Kasukawa, Takeya; Kawaji, Hideya; Carninci, Piero; Forrest, Alistair R. R.; Hayashizaki, Yoshihide

    2017-01-01

    In the FANTOM5 project, transcription initiation events across the human and mouse genomes were mapped at a single base-pair resolution and their frequencies were monitored by CAGE (Cap Analysis of Gene Expression) coupled with single-molecule sequencing. Approximately three thousands of samples,

  11. Protein Profile study of clinical samples using Laser Induced Fluorescence as the detection method

    DEFF Research Database (Denmark)

    Karemore, Gopal Raghunath; Raja, Sujatha N.; Rai, Lavanya

    2009-01-01

      Protein profiles of tissue homogenates were recorded using HPLC separation and LIF detection method. The samples were collected from volunteers with clinically normal or cervical cancer conditions. It is shown that the protein profile can be classified as belonging to malignant or normal state ...

  12. 75 FR 39680 - Houston Pipe Line Company LP, Worsham-Steed Gas Storage, L.P., Energy Transfer Fuel, LP, Mid...

    Science.gov (United States)

    2010-07-12

    ... Storage, L.P., Energy Transfer Fuel, LP, Mid Continent Market Center, L.L.C., Oasis Pipeline, LP (Not... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. PR10-44-000; Docket No. PR10-46-000; Docket No. PR10-48...

  13. Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples

    Directory of Open Access Journals (Sweden)

    Daniel Belstrøm

    2016-03-01

    Full Text Available Background and objective: The microbial profiles of stimulated saliva samples have been shown to differentiate between patients with periodontitis, patients with dental caries, and orally healthy individuals. Saliva was stimulated to allow for easy and rapid collection; however, microbial composition may not reflect the more natural, unstimulated state. The purpose of this study was to validate whether stimulated saliva is an adequate surrogate for unstimulated saliva in determining salivary microbiomes. Design: Unstimulated (n=20 and stimulated (n=20 saliva samples were collected from 20 orally and systemically healthy, non-smoking participants. Salivary bacterial profiles were analyzed by means of the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS, and statistical analysis was performed using Mann–Whitney test with Benjamini–Hochberg's correction for multiple comparison, cluster analysis, principal component analysis, and correspondence analysis. Results: From a total of 40 saliva samples, 496 probe targets were identified with a mean number of targets per sample of 203 (range: 146–303, and a mean number of probe targets of 206 and 200 in unstimulated and stimulated saliva samples, respectively (p=0.62. Based on all statistical methods used for this study, the microbial profiles of unstimulated and stimulated saliva samples collected from the same person were not statistically significantly different. Conclusions: Analysis of bacterial salivary profiles in unstimulated and stimulated saliva samples collected from the same individual showed comparable results. Thus, the results verify that stimulated saliva is an adequate surrogate of unstimulated saliva for microbiome-related studies.

  14. Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples

    DEFF Research Database (Denmark)

    Belstrøm, Daniel; Holmstrup, Palle; Jensen, Allan Bardow

    2016-01-01

    BACKGROUND AND OBJECTIVE: The microbial profiles of stimulated saliva samples have been shown to differentiate between patients with periodontitis, patients with dental caries, and orally healthy individuals. Saliva was stimulated to allow for easy and rapid collection; however, microbial...... orally and systemically healthy, non-smoking participants. Salivary bacterial profiles were analyzed by means of the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS), and statistical analysis was performed using Mann-Whitney test with Benjamini-Hochberg's correction...

  15. Maternal influences on the transmission of leukocyte gene expression profiles in population samples from Brisbane, Australia.

    Directory of Open Access Journals (Sweden)

    Elizabeth Mason

    Full Text Available Two gene expression profiling studies designed to identify maternal influences on development of the neonate immune system and to address the population structure of the leukocyte transcriptome were carried out in Brisbane, Australia. In the first study, a comparison of 19 leukocyte samples obtained from mothers in the last three weeks of pregnancy with 37 umbilical cord blood samples documented differential expression of 7,382 probes at a false discovery rate of 1%, representing approximately half of the expressed transcriptome. An even larger component of the variation involving 8,432 probes, notably enriched for Vitamin E and methotrexate-responsive genes, distinguished two sets of individuals, with perfect transmission of the two profile types between each of 16 mother-child pairs in the study. A minor profile of variation was found to distinguish the gene expression profiles of obese mothers and children of gestational diabetic mothers from those of children born to obese mothers. The second study was of adult leukocyte profiles from a cross-section of Red Cross blood donors sampled throughout Brisbane. The first two axes in this study are related to the third and fourth axes of variation in the first study and also reflect variation in the abundance of CD4 and CD8 transcripts. One of the profiles associated with the third axis is largely excluded from samples from the central portion of the city. Despite enrichment of insulin signaling and aspects of central metabolism among the differentially expressed genes, there was little correlation between leukocyte expression profiles and body mass index overall. Our data is consistent with the notion that maternal health and cytokine milieu directly impact gene expression in fetal tissues, but that there is likely to be a complex interplay between cultural, genetic, and other environmental factors in the programming of gene expression in leukocytes of newborn children.

  16. Microbial profile comparisons of saliva, pooled and site-specific subgingival samples in periodontitis patients

    DEFF Research Database (Denmark)

    Belstrøm, Daniel; Sembler-Møller, Maria Lynn; Grande, Maria Anastasia

    2017-01-01

    OBJECTIVES: The purpose of this study was to compare microbial profiles of saliva, pooled and site-specific subgingival samples in patients with periodontitis. We tested the hypotheses that saliva can be an alternative to pooled subgingival samples, when screening for presence of periopathogens....... DESIGN: Site specific subgingival plaque samples (n = 54), pooled subgingival plaque samples (n = 18) and stimulated saliva samples (n = 18) were collected from 18 patients with generalized chronic periodontitis. Subgingival and salivary microbiotas were characterized by means of HOMINGS (Human Oral...... to an AUC of 0.76 (sensitivity: 0.56, specificity: 0.94) in pooled subgingival samples. CONCLUSIONS: Site-specific presence of periodontal pathogens was detected with comparable accuracy in stimulated saliva samples and pooled subgingival plaque samples. Consequently, saliva may be a reasonable surrogate...

  17. Different organochlorine contaminant profiles in groups of flounders (Platichthys flesus) from sampling locations around Denmark

    DEFF Research Database (Denmark)

    Vorkamp, Katrin; Svendsen, Tore Christian; Rønsholdt, Bent

    2012-01-01

    Flounders (Platichthys flesus) from the waters around Denmark were analysed for their organochlorine (OC) profile to study whether fish from the same genetic population could be separated into characteristic subpopulations, based on their feeding grounds. The chemical analysis of fish liver...... provided a data set of 16 OC compounds in 94 samples from 2004 to 2006. Except for hexachlorocyclohexane, OC compounds were intercorrelated, indicating similar environmental fate and bioaccumulation. OC profiles are less affected than absolute concentrations by potentially confounding biological factors...... and thus more suitable for studies of intrapopulation differences in relation to feeding grounds. Principal component analysis grouped the samples according to locations. All but three of the 94 samples could be reclassified. Samples from the same and additional locations collected in 2003 provided...

  18. Profiles of Chronic Illness Knowledge in a Community Sample of American Adults

    Science.gov (United States)

    Jackson, Todd

    2009-01-01

    The author identified profiles of chronic illness knowledge (i.e., heart disease, cancer, diabetes) in a community sample of American adults and examined the effect of sociodemographic influences on relations of illness knowledge to health practices and well-being. Participants were 181 women and 120 men who completed measures of illness…

  19. Microbial profile comparisons of saliva, pooled and site-specific subgingival samples in periodontitis patients

    DEFF Research Database (Denmark)

    Belstrøm, Daniel; Sembler-Møller, Maria Lynn; Grande, Maria Anastasia

    2017-01-01

    . DESIGN: Site specific subgingival plaque samples (n = 54), pooled subgingival plaque samples (n = 18) and stimulated saliva samples (n = 18) were collected from 18 patients with generalized chronic periodontitis. Subgingival and salivary microbiotas were characterized by means of HOMINGS (Human Oral......OBJECTIVES: The purpose of this study was to compare microbial profiles of saliva, pooled and site-specific subgingival samples in patients with periodontitis. We tested the hypotheses that saliva can be an alternative to pooled subgingival samples, when screening for presence of periopathogens...... by pooled subgingival samples. Presence of Porphyromonas gingivalis, Treponema denticola, Prevotella intermedia, Filifactor alocis, Tannerella forsythia and Parvimona micra in site-specific subgingival samples were detected in saliva with an AUC of 0.79 (sensitivity: 0.61, specificity: 0.94), compared...

  20. Immunomodulatory Effects of Lactobacillus plantarum Lp62 on Intestinal Epithelial and Mononuclear Cells

    Directory of Open Access Journals (Sweden)

    Thalis Ferreira dos Santos

    2016-01-01

    Full Text Available Probiotic lactic acid bacteria are known for their ability to modulate the immune system. They have been shown to inhibit inflammation in experiments with animal models, cell culture, and clinical trials. The objective of this study was to elucidate the anti-inflammatory potential of Lactobacillus plantarum Lp62, isolated from cocoa fermentation, in a cell culture model. Lp62 inhibited IL-8 production by Salmonella Typhi-stimulated HT-29 cells and prevented the adhesion of pathogens to these epithelial cells. The probiotic strain was able to modulate TNF-α, IL1-β, and IL-17 secretion by J774 macrophages. J774 activation was reduced by coincubation with Lp62. PBMC culture showed significantly higher levels of CD4+CD25+ T lymphocytes following treatment with Lp62. Probiotics also induced increased IL-10 secretion by mononuclear cells. L. plantarum Lp62 was able to inhibit inflammatory stimulation in epithelial cells and macrophages and activated a tolerogenic profile in mononuclear cells of healthy donors. These results indicate this strain for a possible application in the treatment or prevention of inflammatory diseases.

  1. A unified treatment for Lp Brunn-Minkowski type inequalities

    OpenAIRE

    Zou, Du; Xiong, Ge

    2016-01-01

    A unified approach used to generalize classical Brunn-Minkowski type inequalities to Lp Brunn-Minkowski type inequalities, called the Lp transference principle, is refined in this paper. As illustrations of the effectiveness and practicability of this method, several new Lp Brunn-Minkowski type inequalities concerning the mixed volume, moment of inertia, quermassintegral, projection body and capacity are established.

  2. Sterile paper points as a bacterial DNA-contamination source in microbiome profiles of clinical samples.

    Science.gov (United States)

    van der Horst, Joyce; Buijs, Mark J; Laine, Marja L; Wismeijer, Daniël; Loos, Bruno G; Crielaard, Wim; Zaura, Egija

    2013-12-01

    High throughput sequencing of bacterial DNA from clinical samples provides untargeted, open-ended information on the entire microbial community. The downside of this approach is the vulnerability to DNA contamination from other sources than the clinical sample. Here we describe contamination from sterile paper points (PPs) used in microbial sample collection. Peri-implant samples from 48 individuals were collected using sterile PPs. Control samples contained only PPs or DNA extraction blank controls. 16S rRNA gene libraries were sequenced using 454 pyrosequencing. 16S rRNA gene copy numbers were measured by quantitative PCR. Nearly half of the sequencing reads belonged to two OTUs classified as Enterococcus (25% of reads) or Exiguobacterium (21%), which are not typical oral microorganisms. Of 87 peri-implant samples, only 10 samples (11%) contained neither of the two OTUs. The relative abundance of both unusual OTUs correlated with each other (pDNA equivalent to 1.2 × 10(3) and 1.1 × 10(4) cells respectively, which was within the range of DNA in the clinical samples (average 1.8 × 10(7), SD 4.8 × 10(7), min 4.4 × 10(2), max 2.8 × 10(8)). The microbial profile from these PPs was dominated (>83% of reads) by the two unusual OTUs. Sterile PPs can contain contaminating bacterial DNA. The use of PPs as a sampling tool for microbial profiling of clinical samples by open-ended techniques such as sequencing or DGGE should be avoided. Clinicians working with PPs as sampling tools for bacterial DNA should consider using an alternative sampling tool, because sterile unused PPs can be a considerable source of foreign bacterial DNA. We recommend sterile curettes for collecting clinical samples for open-ended techniques, such as sequencing or DGGE. Copyright © 2013 Elsevier Ltd. All rights reserved.

  3. Language profiles in young children with autism spectrum disorder: A community sample using multiple assessment instruments.

    Science.gov (United States)

    Nevill, Rose; Hedley, Darren; Uljarević, Mirko; Sahin, Ensu; Zadek, Johanna; Butter, Eric; Mulick, James A

    2017-11-01

    This study investigated language profiles in a community-based sample of 104 children aged 1-3 years who had been diagnosed with autism spectrum disorder using Diagnostic and Statistical Manual of Mental Disorders (5th ed.) diagnostic criteria. Language was assessed with the Mullen scales, Preschool Language Scale, fifth edition, and Vineland-II parent-report. The study aimed to determine whether the receptive-to-expressive language profile is independent from the assessment instrument used, and whether nonverbal cognition, early communicative behaviors, and autism spectrum disorder symptoms predict language scores. Receptive-to-expressive language profiles differed between assessment instruments and reporters, and Preschool Language Scale, fifth edition profiles were also dependent on developmental level. Nonverbal cognition and joint attention significantly predicted receptive language scores, and nonverbal cognition and frequency of vocalizations predicted expressive language scores. These findings support the administration of multiple direct assessment and parent-report instruments when evaluating language in young children with autism spectrum disorder, for both research and in clinical settings. Results also support that joint attention is a useful intervention target for improving receptive language skills in young children with autism spectrum disorder. Future research comparing language profiles of young children with autism spectrum disorder to children with non-autism spectrum disorder developmental delays and typical development will add to our knowledge of early language development in children with autism spectrum disorder.

  4. MicroRNA profiling of pericardial fluid samples from patients with heart failure.

    Directory of Open Access Journals (Sweden)

    Suvi M Kuosmanen

    Full Text Available Multicellular organisms maintain vital functions through intercellular communication. Release of extracellular vesicles that carry signals to even distant target organs is one way of accomplishing this communication. MicroRNAs can also be secreted from the cells in exosomes and act as paracrine signalling molecules. In addition, microRNAs have been implicated in the pathogenesis of a large number of diseases, including cardiovascular diseases, and are considered as promising candidate biomarkers due to their relative stability and easy quantification from clinical samples. Pericardial fluid contains hormones secreted by the heart and is known to reflect the cardiac function. In this study, we sought to investigate whether pericardial fluid contains microRNAs and if so, whether they could be used to distinguish between different cardiovascular pathologies and disease stages.Pericardial fluid was collected from heart failure patients during open-heart surgery. MicroRNA profiles of altogether 51 patients were measured by quantitative real-time PCR (qPCR using Exiqon human panels I and II. On the average, 256 microRNAs were detected per sample, and 70 microRNAs out of 742 profiled microRNAs were detected in every sample. The five most abundant microRNAs in pericardial fluid were miR-21-5p, miR-451a, miR-125b-5p, let-7b-5p and miR-16-5p. No specific signatures for cardiovascular pathologies or clinically assessed heart failure stages could be detected from the profiles and, overall, microRNA profiles of the samples were found to be very similar despite the heterogeneity in the study population.Measured microRNA profiles did not separate the samples according to the clinical features of the patients. However, several previously identified heart failure marker microRNAs were detected. The pericardial fluid microRNA profile appeared to be a result of an active and selective secretory process indicating that microRNAs may act as paracrine signalling

  5. Personality profiles and problematic internet use in a sample of Italian adolescents

    OpenAIRE

    Fanny Guglielmucci; Antonella Granieri; Donato Munno; Giuseppina Zullo; Marta Saroldi

    2017-01-01

    Objective: The aim of the present study was to investigate the relationship between specific personality profiles and Internet use in a sample of Italian adolescents. Method: Four hundred thirty-two adolescents (58.3% males) with an average age of 14.41 years (SD=.95) were enrolled in the study. Participants were administered the Internet Addiction Test (IAT) and the Minnesota Multiphasic Inventory – Adolescent Form (MMPI-A). A two-step cluster analysis was relied according to IAT items’ ...

  6. 75 FR 20870 - Montreux Equity Partners II SBIC, L.P.; Montreux Equity Partners III SBIC, L.P.; Notice Seeking...

    Science.gov (United States)

    2010-04-21

    ... ADMINISTRATION Montreux Equity Partners II SBIC, L.P.; Montreux Equity Partners III SBIC, L.P.; Notice Seeking... given that Montreux Equity Partners II SBIC, L.P. and Montreux Equity Partners III SBIC, L.P., 3000 Sand...). Montreux Equity Partners III SBIC, L.P. proposes to sell part of its equity interest in Renal CarePartners...

  7. The relation between Lp-PLA2 levels with periodic limb movements.

    Science.gov (United States)

    Bekci, Taha Tahir; Kayrak, Mehmet; Kiyici, Aysel; Ari, Hatem; Teke, Turgut; Maden, Emin; Akilli, Hakan

    2012-03-01

    Lipoprotein-associated phospholipase A2 (Lp-PLA2), a novel marker of vulnerable plaque to prone rupture, is a predictor of both cardiovascular event and cerebrovascular event, and highly sensitive-C-reactive protein (hs-CRP) is an acute-phase response protein implicated in a broad range of cardiovascular diseases. We aimed to examine the association between periodic limb movements in sleep (PLMs) with circulating Lp-PLA2 and hs-CRP levels in patients with PLMs. Seventy patients with newly diagnosed PLM with polysomnography were enrolled this study. Patients were divided into two groups according to PLM index (normal PLM index, PLM index, ≥15). Lp-PLA2 and hs-CRP concentrations were measured in serum samples by turbidimetric and nephelometric methods, respectively. The concentrations of these parameters were compared between two groups and correlation analysis was performed between PLMs and Lp-PLA2 and hs-CRP levels. Lp-PLA2 levels and hs-CRP were significantly increased in elevated PLM index group compared with the control group (206.8 ± 78.1 vs 157.8 ± 56.7, p = 0.003, and 4.2 ± 3.5 vs 2.4 ± 2.1, p = 0.02, respectively). PLM index was positively correlated with Lp-PLA2 levels (r = 0.40, p = 0.001) and hs-CRP (r = 0.24, p = 0.05). In the linear regression model, Lp-PLA2 was an independent predictor of PLM index (R(2) = 0.36, p = 0.005). This study demonstrated an independent linear relation between PLM index and Lp-PLA2. In addition, it was seen increased Lp-PLA2 and hs-CRP levels in patients with elevated PLM index. Based on these results, we can suggest that risk of vascular events may be increased in patients with PLMs and with increased PLM index.

  8. Validation of refractivity profiles derived from GRAS raw-sampling data

    Directory of Open Access Journals (Sweden)

    F. Zus

    2011-07-01

    Full Text Available Results from GRAS (GNSS Receiver for Atmospheric Sounding RO (Radio Occultation data recorded in RS (Raw Sampling mode processed at the GFZ (German Research Centre for Geoscience Potsdam are presented. The experimental processing software POCS-X includes FSI (Full Spectrum Inversion in order to cope with multi-path regions and enables in connection with RS data to retrieve atmospheric refractivity profiles down to the Earths surface. Radio occultation events observed between 30 September and 30 October 2007 are processed and the retrievals are validated against co-located ECMWF (European Centre for Medium-Range Weather Forecasts profiles. The intercomparison indicates good quality of the retrieved profiles. In the altitude range 8 to 25 km the standard deviation is below 1 %. The mean deviation in this altitude range tends to be negative. At 30 km the negative bias reaches about −0.4 %. Below 8 km the standard deviation increases, reaching 2.5 % at 2 km. Below 2 km the mean deviation tends to be negative, reaching −1.9 % close to the ground. The negative bias mainly stems from the tropical lower troposphere; there, the negative bias reaches −3 %. The tropospheric penetration depth obtained from RS data shows a vast improvement compared to the tropospheric penetration depth typically obtained from CL (Closed Loop data; 50 % of all retrieved profiles reach 720 m.

  9. Language, reading, and math learning profiles in an epidemiological sample of school age children.

    Science.gov (United States)

    Archibald, Lisa M D; Oram Cardy, Janis; Joanisse, Marc F; Ansari, Daniel

    2013-01-01

    Dyscalculia, dyslexia, and specific language impairment (SLI) are relatively specific developmental learning disabilities in math, reading, and oral language, respectively, that occur in the context of average intellectual capacity and adequate environmental opportunities. Past research has been dominated by studies focused on single impairments despite the widespread recognition that overlapping and comorbid deficits are common. The present study took an epidemiological approach to study the learning profiles of a large school age sample in language, reading, and math. Both general learning profiles reflecting good or poor performance across measures and specific learning profiles involving either weak language, weak reading, weak math, or weak math and reading were observed. These latter four profiles characterized 70% of children with some evidence of a learning disability. Low scores in phonological short-term memory characterized clusters with a language-based weakness whereas low or variable phonological awareness was associated with the reading (but not language-based) weaknesses. The low math only group did not show these phonological deficits. These findings may suggest different etiologies for language-based deficits in language, reading, and math, reading-related impairments in reading and math, and isolated math disabilities.

  10. Language, Reading, and Math Learning Profiles in an Epidemiological Sample of School Age Children

    Science.gov (United States)

    Archibald, Lisa M. D.; Oram Cardy, Janis; Joanisse, Marc F.; Ansari, Daniel

    2013-01-01

    Dyscalculia, dyslexia, and specific language impairment (SLI) are relatively specific developmental learning disabilities in math, reading, and oral language, respectively, that occur in the context of average intellectual capacity and adequate environmental opportunities. Past research has been dominated by studies focused on single impairments despite the widespread recognition that overlapping and comorbid deficits are common. The present study took an epidemiological approach to study the learning profiles of a large school age sample in language, reading, and math. Both general learning profiles reflecting good or poor performance across measures and specific learning profiles involving either weak language, weak reading, weak math, or weak math and reading were observed. These latter four profiles characterized 70% of children with some evidence of a learning disability. Low scores in phonological short-term memory characterized clusters with a language-based weakness whereas low or variable phonological awareness was associated with the reading (but not language-based) weaknesses. The low math only group did not show these phonological deficits. These findings may suggest different etiologies for language-based deficits in language, reading, and math, reading-related impairments in reading and math, and isolated math disabilities. PMID:24155959

  11. Language, reading, and math learning profiles in an epidemiological sample of school age children.

    Directory of Open Access Journals (Sweden)

    Lisa M D Archibald

    Full Text Available Dyscalculia, dyslexia, and specific language impairment (SLI are relatively specific developmental learning disabilities in math, reading, and oral language, respectively, that occur in the context of average intellectual capacity and adequate environmental opportunities. Past research has been dominated by studies focused on single impairments despite the widespread recognition that overlapping and comorbid deficits are common. The present study took an epidemiological approach to study the learning profiles of a large school age sample in language, reading, and math. Both general learning profiles reflecting good or poor performance across measures and specific learning profiles involving either weak language, weak reading, weak math, or weak math and reading were observed. These latter four profiles characterized 70% of children with some evidence of a learning disability. Low scores in phonological short-term memory characterized clusters with a language-based weakness whereas low or variable phonological awareness was associated with the reading (but not language-based weaknesses. The low math only group did not show these phonological deficits. These findings may suggest different etiologies for language-based deficits in language, reading, and math, reading-related impairments in reading and math, and isolated math disabilities.

  12. [Distribution and migration of heavy metals in soil profiles by high-resolution sampling].

    Science.gov (United States)

    Ruan, Xin-ling; Zhang, Gan-lin; Zhao, Yu-guo; Yuan, Da-gang; Wu, Yun-jin

    2006-05-01

    The vertical distribution of heavy metals in soils profiles is a result of heavy metals accumulation and migration under combining influence of edaphic factors and environmental conditions. It's an important basis for evaluation of heavy metals pollution and remediation of contaminated soils. By traditional sampling methods, i.e., soils were sampled according to pedogenetic horizons, only very general information about element migration can be learned. In the current study, three sites near a steel factory were selected to represent three types of land use, i.e. forest, dry land for vegetable cultivation and rice paddy field. Soils were sampled horizontally by high-resolution sampling method. In the top of 40 cm soils were sectioned in 2 cm intervals, then 5 cm intervals in next 40 cm, and 10 cm intervals in the last 20 cm of profile. Total content of Cu, Zn, Pb, Cr and Cd were determined, and the vertical distribution of Cu, Zn, Pb, Cr, Cd in every profile was analyzed. The results indicated that enrichment of heavy metals appeared in the upper most layer of the natural forest soil that without any anthropic disturbance, and this phenomenon proved that heavy metals were coming from atmospheric deposition. We found that Cu, Zn and Pb moved downward in a short distance, Cd migrated relatively faster than Cu, Zn and Pb, while Cr had no recognizable location of migration front. In the soil profiles of dry land and paddy field, there were influences of agricultural practice, the distribution and movement of metals were thus different form those of the forest soil. In cultivated layer heavy metals were evenly distributed because soils in the upper layer were mixed by cultivation, however, bellow the cultivated layer obvious migration took place again. It is concluded that different heavy metals have different mobility and there is such a relative order: Cd>Cu>Zn>Pb. The study shows that the distribution pattern can be obtained with the currently adopted high

  13. Serum LP(A) levels in randomized healthy men from different European countries

    NARCIS (Netherlands)

    Cigolini, M; Seidell, J C; Zenti, M G; Bonadonna, G; Zambelli, L; Deslypere, J P; Contaldo, F; Cruz, A.; Charzewska, J; Targher, G

    Serum lipoprotein(a) [Lp(a)], blood lipids, serum insulin and anthropometric parameters were determined in randomized samples of 38-year-old men living in six European cities: Ede (The Netherlands), Deinze (Belgium), Warsaw (Poland), Lumiar (Portugal), Verona and Naples (respectively in northern and

  14. Whole-genome gene expression profiling of formalin-fixed, paraffin-embedded tissue samples.

    Directory of Open Access Journals (Sweden)

    Craig April

    2009-12-01

    Full Text Available We have developed a gene expression assay (Whole-Genome DASL, capable of generating whole-genome gene expression profiles from degraded samples such as formalin-fixed, paraffin-embedded (FFPE specimens.We demonstrated a similar level of sensitivity in gene detection between matched fresh-frozen (FF and FFPE samples, with the number and overlap of probes detected in the FFPE samples being approximately 88% and 95% of that in the corresponding FF samples, respectively; 74% of the differentially expressed probes overlapped between the FF and FFPE pairs. The WG-DASL assay is also able to detect 1.3-1.5 and 1.5-2 -fold changes in intact and FFPE samples, respectively. The dynamic range for the assay is approximately 3 logs. Comparing the WG-DASL assay with an in vitro transcription-based labeling method yielded fold-change correlations of R(2 approximately 0.83, while fold-change comparisons with quantitative RT-PCR assays yielded R(2 approximately 0.86 and R(2 approximately 0.55 for intact and FFPE samples, respectively. Additionally, the WG-DASL assay yielded high self-correlations (R(2>0.98 with low intact RNA inputs ranging from 1 ng to 100 ng; reproducible expression profiles were also obtained with 250 pg total RNA (R(2 approximately 0.92, with approximately 71% of the probes detected in 100 ng total RNA also detected at the 250 pg level. When FFPE samples were assayed, 1 ng total RNA yielded self-correlations of R(2 approximately 0.80, while still maintaining a correlation of R(2 approximately 0.75 with standard FFPE inputs (200 ng.Taken together, these results show that WG-DASL assay provides a reliable platform for genome-wide expression profiling in archived materials. It also possesses utility within clinical settings where only limited quantities of samples may be available (e.g. microdissected material or when minimally invasive procedures are performed (e.g. biopsied specimens.

  15. High Resolution Hydraulic Profiling and Groundwater Sampling using FLUTe™ System in a Fractured Limestone Setting

    DEFF Research Database (Denmark)

    Janniche, Gry Sander; Christensen, Anders G.; Grosen, Bernt

    innovative investi-gation methods for characterization of the source zone hydrogeology and contamination, including FLUTe system hydraulic profiling and Water-FLUTe multilevel groundwater sampling, in fractured bryo-zoan limestone bedrock. High resolution hydraulic profiling was conducted in three cored......Characterization of the contaminant source zone architecture and the hydraulics is essential to develop accurate site specific conceptual models, delineate and quantify contaminant mass, perform risk as-sessment, and select and design remediation alternatives. This characterization is particularly...... challeng-ing in deposit types as fractured limestone. The activities of a bulk distribution facility for perchloroe-thene (PCE) and trichloroethene (TCE) at the Naverland site near Copenhagen, Denmark, has resulted in PCE and TCE DNAPL impacts to a fractured clay till and an underlying fractured limestone...

  16. Sampling Modification Effects in the Subgingival Microbiome Profile of Healthy Children.

    Science.gov (United States)

    Santigli, Elisabeth; Trajanoski, Slave; Eberhard, Katharina; Klug, Barbara

    2016-01-01

    sampling modes ( p = 0.914 by R 2 = 0.09). Conclusion: A slight modification of sampling mode has minor effects corresponding to a natural variability in the microbiome profiles of healthy children. The inter-individual variability in subgingival microprints is greater than intra-individual differences. Statistical analyses of microbial populations should consider this baseline variability and move beyond mere quantification with input from visual analytics. Comparative results are difficult to summarize as methods for studying huge datasets are still evolving. Advanced approaches are needed for sample size calculations in clinical settings.

  17. Sampling Modification Effects in the Subgingival Microbiome Profile of Healthy Children

    Science.gov (United States)

    Santigli, Elisabeth; Trajanoski, Slave; Eberhard, Katharina; Klug, Barbara

    2017-01-01

    grouping by sampling modes (p = 0.914 by R2 = 0.09). Conclusion: A slight modification of sampling mode has minor effects corresponding to a natural variability in the microbiome profiles of healthy children. The inter-individual variability in subgingival microprints is greater than intra-individual differences. Statistical analyses of microbial populations should consider this baseline variability and move beyond mere quantification with input from visual analytics. Comparative results are difficult to summarize as methods for studying huge datasets are still evolving. Advanced approaches are needed for sample size calculations in clinical settings. PMID:28149291

  18. The Land Processes Distributed Active Archive Center (LP DAAC)

    Science.gov (United States)

    Golon, Danielle K.

    2016-10-03

    The Land Processes Distributed Active Archive Center (LP DAAC) operates as a partnership with the U.S. Geological Survey and is 1 of 12 DAACs within the National Aeronautics and Space Administration (NASA) Earth Observing System Data and Information System (EOSDIS). The LP DAAC ingests, archives, processes, and distributes NASA Earth science remote sensing data. These data are provided to the public at no charge. Data distributed by the LP DAAC provide information about Earth’s surface from daily to yearly intervals and at 15 to 5,600 meter spatial resolution. Data provided by the LP DAAC can be used to study changes in agriculture, vegetation, ecosystems, elevation, and much more. The LP DAAC provides several ways to access, process, and interact with these data. In addition, the LP DAAC is actively archiving new datasets to provide users with a variety of data to study the Earth.

  19. Latent profile analysis and comorbidity in a sample of individuals with compulsive buying disorder.

    Science.gov (United States)

    Mueller, Astrid; Mitchell, James E; Black, Donald W; Crosby, Ross D; Berg, Kelly; de Zwaan, Martina

    2010-07-30

    The aims of this study were to perform a latent profile analysis in a sample of individuals with compulsive buying, to explore the psychiatric comorbidity, and to examine whether or not more severe compulsive buying is associated with greater comorbidity. Compulsive buying measures and SCID data obtained from 171 patients with compulsive buying behavior who had participated in treatment trials at different clinical centers in the U.S. and Germany were analyzed. Latent profile analysis produced two clusters. Overall, cluster 2, included subjects with more severe compulsive buying, and was characterized by higher lifetime as well as current prevalence rates for Axis I and impulse control disorders. Nearly 90% of the total sample reported at least one lifetime Axis I diagnosis, particularly mood (74%) and anxiety (57%) disorders. Twenty-one percent had a comorbid impulse control disorder, most commonly intermittent explosive disorder (11%). Half of the sample presented with at least one current Axis I disorder, most commonly anxiety disorders (44%). Given the substantial psychiatric comorbidity, it is reasonable to question whether or not compulsive buying represents a distinct psychiatric entity vs. an epiphenomenon of other psychiatric disorders. Copyright 2010 Elsevier Ltd. All rights reserved.

  20. Survival associated pathway identification with group Lp penalized global AUC maximization

    Directory of Open Access Journals (Sweden)

    Liu Zhenqiu

    2010-08-01

    Full Text Available Abstract It has been demonstrated that genes in a cell do not act independently. They interact with one another to complete certain biological processes or to implement certain molecular functions. How to incorporate biological pathways or functional groups into the model and identify survival associated gene pathways is still a challenging problem. In this paper, we propose a novel iterative gradient based method for survival analysis with group Lp penalized global AUC summary maximization. Unlike LASSO, Lp (p 1. We first extend Lp for individual gene identification to group Lp penalty for pathway selection, and then develop a novel iterative gradient algorithm for penalized global AUC summary maximization (IGGAUCS. This method incorporates the genetic pathways into global AUC summary maximization and identifies survival associated pathways instead of individual genes. The tuning parameters are determined using 10-fold cross validation with training data only. The prediction performance is evaluated using test data. We apply the proposed method to survival outcome analysis with gene expression profile and identify multiple pathways simultaneously. Experimental results with simulation and gene expression data demonstrate that the proposed procedures can be used for identifying important biological pathways that are related to survival phenotype and for building a parsimonious model for predicting the survival times.

  1. Impurity profiling/comparative analyses of samples of 1-phenyl-2-propanone.

    Science.gov (United States)

    Krawczyk, W; Kunda, T; Perkowska, I; Dudek, D

    2005-01-01

    1-Phenyl-2-propanone (P-2-P), also known as benzyl methyl ketone (BMK), is the main precursor used in amphetamine synthesis. In recent years, the number of seizures of P-2-P from both licit and illicit drug manufacture has increased. The present article comprises a discussion of some of the largest seizures of P-2-P diverted from regular production to the illicit market. It also presents the methods used in clandestine laboratories to synthesize P-2-P and a forensic approach to identify and differentiate between these methods. To that end, and to facilitate the monitoring of the P-2-P market, a method of P-2-P impurity profiling was designed for comparative purposes and for the identification of the synthesis route. P-2-P samples were analysed by means of gas chromatography/mass spectrometry (GC/MS). Out of 36 identified impurities, 14 were selected as markers for sample comparison. On the basis of the GC peak areas of those 14 markers, a cluster analysis was carried out, resulting in three clusters, each corresponding to a given P-2-P synthesis route. The results of P-2-P impurity profiling are stored in both a forensic database and a police database. The forensic database comprises chemical data, such as those on P-2-P purity, additives and specific impurities, as well as information on seized P-2-P samples having a similar impurity profile. Data stored in the police database, which is linked with the forensic database by case identification number, cover the circumstances of seizures and personal details of offenders. The databases enable the full use of forensic data in intelligence work and police investigative activities.

  2. Effects of tetracycline administration on the proteomic profile of pig muscle samples (L. dorsi)

    DEFF Research Database (Denmark)

    Gratacos-Cubarsi, M.; Castellari, M.; Hortos, M.

    2008-01-01

    Effect of tetracycline (TC) administration on the proteomic profile of pig muscle was evaluated by 2D electrophoresis and MALDI-TOF mass spectrometry. The TC content at slaughter was determined in L. dorsi samples by HPLC-DAD. Mean residual concentration of TC in the muscle of treated animals......, calculated as the sum of TC and epi-TC was 126.3 mu g/kg, indicating a rapid elimination of TC in this tissue. Several differential spots (n = 54, p match for 5 differential spots, that is...

  3. Observations from a 4-Year Contamination Study of a Sample Depth Profile Through Martian Meteorite Nakhla

    Science.gov (United States)

    Toporski,Jan; Steele, Andrew

    2007-05-01

    Morphological, compositional, and biological evidence indicates the presence of numerous well-developed microbial hyphae structures distributed within four different sample splits of the Nakhla meteorite obtained from the British Museum (allocation BM1913,25). By examining depth profiles of the sample splits over time, morphological changes displayed by the structures were documented, as well as changes in their distribution on the samples, observations that indicate growth, decay, and reproduction of individual microorganisms. Biological staining with DNA-specific molecular dyes followed by epifluorescence microscopy showed that the hyphae structures contain DNA. Our observations demonstrate the potential of microbial interaction with extraterrestrial materials, emphasize the need for rapid investigation of Mars return samples as well as any other returned or impactor-delivered extraterrestrial materials, and suggest the identification of appropriate storage conditions that should be followed immediately after samples retrieved from the field are received by a handling/curation facility. The observations are further relevant in planetary protection considerations as they demonstrate that microorganisms may endure and reproduce in extraterrestrial materials over long (at least 4 years) time spans. The combination of microscopy images coupled with compositional and molecular staining techniques is proposed as a valid method for detection of life forms in martian materials as a first-order assessment. Time-resolved in situ observations further allow observation of possible (bio)dynamics within the system.

  4. Temperament and Character Profiles of Sasang Typology in an Adult Clinical Sample

    Directory of Open Access Journals (Sweden)

    Soo Hyun Park

    2011-01-01

    Full Text Available The purpose of this study was to examine the biopsychological personality profiles of traditional Korean Sasang typology based on the Temperament and Character Inventory (TCI in a Korean adult clinical sample. A total of 97 adults completed the Korean version of the TCI. The participants were classified as one of three traditional Korean Sasang types (31 So-Yang, 41 Tae-Eum, 25 So-Eum by three specialists in Sasang typology. The seven dimensions of TCI were compared between the different Sasang types using analysis of variance (ANOVA and profile analysis. There were no significant differences in age, gender and education across the Sasang types. The TCI profile for each of the Sasang types was significantly different (profile analysis, df = 5.038, F = 3.546, P = .004. There were significant differences in the temperament dimensions of Novelty Seeking (F = 3.43, P = .036 and Harm Avoidance (F = 5.43, P = .006 among the Sasang types. The Novelty Seeking score of the So-Yang type (31.90 ± 9.87 was higher than that of the So-Eum type (25.24 ± 9.21; P = .019 while the So-Eum type (44.64 ± 8.47 scored higher on the Harm Avoidance score compared to the So-Yang type (35.16 ± 11.50; P = .003. There were no significant differences in the temperament dimension of Reward Dependence and Persistence, and the three character dimensions of Self-Directedness, Cooperativeness and Self-Transcendence. Results demonstrated distinct temperament traits associated with traditional Korean Sasang types using an objective biopsychological personality inventory. With further study, the Sasang typology may lead to enhanced clinical safety and efficacy as part of personalized medicine with traditional medicine.

  5. Investigation of Pectenotoxin Profiles in the Yellow Sea (China Using a Passive Sampling Technique

    Directory of Open Access Journals (Sweden)

    Zhaoxin Li

    2010-04-01

    Full Text Available Pectenotoxins (PTXs are a group of lipophilic algal toxins. These toxins have been found in algae and shellfish from Japan, New Zealand, Ireland, Norway and Portugal. PTX profiles vary with geographic location of collection site. The aim of the present study was to investigate PTX profiles from the Yellow Sea, China. The sampling location was within an aquatic farm (N36°12.428´, E120°17.826´ near the coast of Qingdao, China, in the Yellow Sea from 28July to 29August 2006. PTXs in seawater were determined using a solid phase adsorption toxin tracking (SPATT method. PTXs were analyzed by HPLC-MSMS. PTX-2, PTX-2 sec acid (PTX-2 SA and 7-epi-PTX-2 SA were found in seawater samples. The highest levels of PTXs (107 ng/g of resin PTX-2, 50 ng/g of resin PTX-2 SA plus 7-epi-PTX-2 SA in seawater were found on 1 August, 2006. From 1 August to 29 August, the levels of PTX-2 and PTX-2 SA decreased. In the same area, the marine algae, Dinophysis acuminata was found in the seawater in the summer months of 2006. This indicated that Dinophysis acuumuta might be the original source of PTXs. PTX-11 and PTX-12a/b were not found in seawater.

  6. Efficient recovery of electrophoretic profiles of nucleoside metabolites from urine samples by multivariate curve resolution.

    Science.gov (United States)

    Szymańska, Ewa; Markuszewski, Michał J; Vander Heyden, Yvan; Kaliszan, Roman

    2009-10-01

    Chemometric techniques usually employed in purity assessment and resolution of multicomponent peaks have been applied to analytical data from complex biological samples obtained with CE-DAD. In the assessment of the purity of the electrophoretic peaks, the orthogonal projection approach, the orthogonal projection approach with Durbin-Watson criterion, and the simple-to-use interactive self-modeling mixture analysis method have been employed. Multivariate curve resolution with alternating least squares has been successfully implemented to resolve co-migrating peaks of metabolites in CE-DAD and to recover qualitative and quantitative information about co-migrating components of urine extract. The main challenge consisted of developing high-quality multivariate curve resolution with alternating least squares models of multicomponent peaks acquired during the CE analysis of nucleoside patterns in 18 urine samples. The recovered ultraviolet visible (UV-Vis) spectra have been employed to identify additional nucleosides, such as 1-methylinosine, 2-methylguanosine, and 1-methylguanosine, whose presence in the metabolic profile produced by the applied CE-DAD method has not yet been recognized. Concentration profiles of these compounds can be used in metabonomic studies.

  7. Comorbidity profiles and inpatient outcomes during hospitalization for heart failure: an analysis of the U.S. Nationwide inpatient sample

    OpenAIRE

    Lee, Christopher S.; Chien, Christopher V.; Bidwell, Julie T.; Gelow, Jill M.; Denfeld, Quin E.; Creber, Ruth Masterson; Buck, Harleah G.; Mudd, James O.

    2014-01-01

    Background Treatment of heart failure (HF) is particularly complex in the presence of comorbidities. We sought to identify and associate comorbidity profiles with inpatient outcomes during HF hospitalizations. Methods Latent mixture modeling was used to identify common profiles of comorbidities during adult hospitalizations for HF from the 2009 Nationwide Inpatient Sample (n = 192,327). Results Most discharges were characterized by "common" comorbidities. A "lifestyle" profile was characteriz...

  8. Establishment of a clinical pathway for home enteral nutrition prescription in Murcia. Profile and sample characteristics

    Science.gov (United States)

    Ferrer Gómez, Mercedes; Sánchez Romera, Juan Francisco; García Zafra, María Victoria; Cuenca Sánchez, José Ramón; Hernández Cascales, Ana Belén; Aranda García, Ana; Rausell Rausell, Víctor José; Hernández Martínez, Antonio Miguel

    2017-06-05

    Introduction: Before 2010, prescribed home enteral nutrition (HEN) in Murcia was characterized by the great variability of the receptor patients, in addition to a higher use compared with other geographical areas. Objectives: Developing and describing a clinical pathway for attending candidates for HEN, and analyzing their profi le and prescription characteristics. Methods: Establishment of a clinical pathway for HEN prescription. Bidirectional observational study of the samples of HEN in a specific area (Health Area I of the Region of Murcia) during 2010 (HEN1) and 2013-14 (HEN2). Results: An official management statement was established, generalizing the clinical pathway for the rest of the regional areas (Instruction no. 4/2012 of July 12 th). Although most prevalent diseases in both samples were neurological, followed, with a wide spread, by oncological and digestive cases, there was a signifi cant difference regarding distribution. The HEN1 sample showed a great number of no candidate patients according to the management statement. In both samples, the most prevalent route of administration was oral, but with a trend reversal to feeding tube and gastrostomy in HEN2, where the specifi c formulas were also reduced. Conclusions: The profile of HEN, before and after the deployment of the clinical pathway, changes signifi cantly concerning the main disease, the route of administration and the formula. It has been proved that there is a need for controlling HEN for an appropriate prescription.

  9. Comparison of tear lipid profile among basal, reflex, and flush tear samples.

    Science.gov (United States)

    Rohit, Athira; Stapleton, Fiona; Brown, Simon H J; Mitchell, Todd W; Willcox, Mark D P

    2014-12-01

    To determine whether tear collection by flushing the ocular surface with saline (flush tears) or collection by stimulation (reflex tears) can be used as an alternative to basal tear collection for the identification and quantification of lipids in the tear film. Tear samples were collected from 10 participants with no history of ocular surface disease or contact lens wear. Up to 10 μl of basal, reflex, and flush tear samples were collected from each eye using a microcapillary tube on three occasions with the order of methods randomized and allowing at least 24 hours between each collection method. Lipids were quantified from each tear sample using nano-electrospray ionization tandem mass spectrometry. Total lipids significantly differed in their concentration (pmol/μl) and mole % with each collection technique. Cholesterol esters [mean % (SE)] formed the major component of the total lipidome in basal [54.8% (3.1)], reflex [35.7% (6.4)], and flush [33.0% (3.1)] tear samples. However, the mole % of each lipid class substantially varied with each tear collection method. Nonpolar lipids, including cholesterol, wax esters, and triacylglycerols, dominated the tear lipidome in basal [92.8% (1.9)], reflex [71.8% (7.9)], and flush [83.6% (3.8)] tear samples. However, the mole % of phospholipids in reflex [27.5% (8.1)] and flush [15.8% (3.8)] tear samples was higher (p = 0.005) than that in basal tears [5.4% (2.0)]. Flush or reflex tears did not have similar lipid profiles in either concentration or in mole % to basal tears. It is recommended that basal tears are used for tear lipid analysis as the reflex or flush tears contain very low levels of most lipid components.

  10. miRNA expression profiling enables risk stratification in archived and fresh neuroblastoma tumor samples.

    Science.gov (United States)

    De Preter, Katleen; Mestdagh, Pieter; Vermeulen, Joëlle; Zeka, Fjoralba; Naranjo, Arlene; Bray, Isabella; Castel, Victoria; Chen, Caifu; Drozynska, Elzbieta; Eggert, Angelika; Hogarty, Michael D; Izycka-Swieszewska, Ewa; London, Wendy B; Noguera, Rosa; Piqueras, Marta; Bryan, Kenneth; Schowe, Benjamin; van Sluis, Peter; Molenaar, Jan J; Schramm, Alexander; Schulte, Johannes H; Stallings, Raymond L; Versteeg, Rogier; Laureys, Geneviève; Van Roy, Nadine; Speleman, Frank; Vandesompele, Jo

    2011-12-15

    More accurate assessment of prognosis is important to further improve the choice of risk-related therapy in neuroblastoma (NB) patients. In this study, we aimed to establish and validate a prognostic miRNA signature for children with NB and tested it in both fresh frozen and archived formalin-fixed paraffin-embedded (FFPE) samples. Four hundred-thirty human mature miRNAs were profiled in two patient subgroups with maximally divergent clinical courses. Univariate logistic regression analysis was used to select miRNAs correlating with NB patient survival. A 25-miRNA gene signature was built using 51 training samples, tested on 179 test samples, and validated on an independent set of 304 fresh frozen tumor samples and 75 archived FFPE samples. The 25-miRNA signature significantly discriminates the test patients with respect to progression-free and overall survival (P < 0.0001), both in the overall population and in the cohort of high-risk patients. Multivariate analysis indicates that the miRNA signature is an independent predictor of patient survival after controlling for current risk factors. The results were confirmed in an external validation set. In contrast to a previously published mRNA classifier, the 25-miRNA signature was found to be predictive for patient survival in a set of 75 FFPE neuroblastoma samples. In this study, we present the largest NB miRNA expression study so far, including more than 500 NB patients. We established and validated a robust miRNA classifier, able to identify a cohort of high-risk NB patients at greater risk for adverse outcome using both fresh frozen and archived material. ©2011 AACR.

  11. Trigonometric bases for matrix weighted Lp-spaces

    DEFF Research Database (Denmark)

    Nielsen, Morten

    2010-01-01

    We give a complete characterization of 2π-periodic matrix weights W for which the vector-valued trigonometric system forms a Schauder basis for the matrix weighted space Lp(T;W). Then trigonometric quasi-greedy bases for Lp(T;W) are considered. Quasi-greedy bases are systems for which the simple...

  12. 76 FR 68745 - Seneca Power Partners, L.P.

    Science.gov (United States)

    2011-11-07

    ...] Seneca Power Partners, L.P. v. New York Independent System Operator, Inc.; Notice of Complaint Take... and Procedures, 18 CFR 385.206, Seneca Power Partners, L.P. (Complainant) filed a complaint against... TTY, call (202) 502-8659. Comment Date: 5 p.m. Eastern Time on November 28, 2011. Dated: October 28...

  13. 78 FR 16846 - Notice of Application; Equitrans, L.P.

    Science.gov (United States)

    2013-03-19

    ... Energy Regulatory Commission Notice of Application; Equitrans, L.P. Take notice that on March 1, 2013, Equitrans, L.P. (Equitrans), 625 Liberty Avenue, Suite 1700, Pittsburgh, Pennsylvania 15222, filed in Docket...: 5:00 p.m. Eastern Time on April 2, 2013. Dated: March 12, 2013. Kimberly D. Bose, Secretary. BILLING...

  14. The role of LP gas in eradicating energy poverty

    Energy Technology Data Exchange (ETDEWEB)

    Kelly, Michael; Behuria, Sarthak

    2010-09-15

    LP Gas is an ideal solution for dealing with energy poverty. Clean burning, low carbon, extremely efficient, requiring minimal infrastructure or R and D investment and with plentiful long term global supply, LP Gas can be quickly introduced to play an important role in eradicating energy poverty and steering both industrialised and developing countries onto more sustainable energy development paths.

  15. Flygtninge på langvarig starthjælp

    DEFF Research Database (Denmark)

    Johansen, Adam; Højland, Marianne; Hansen, Henning

    Spørgeskema-, fokusgruppe- og interviewbaseret undersøgelse af personlige og sociale konsekvenser af familiers forsørgelse på starthjælp. Kvalitativ og kvantitativ undersøgelse af starthjælpens konsekvenser for flygtningefamilier med mere end 1 år på starthjælp...

  16. 76 FR 50881 - Airworthiness Directives; M7 Aerospace LP Airplanes

    Science.gov (United States)

    2011-08-17

    ...-025-AD; Amendment 39-16771; AD 2011-17-07] RIN 2120-AA64 Airworthiness Directives; M7 Aerospace LP.... SUMMARY: We are adopting a new airworthiness directive (AD) for certain M7 Aerospace LP Models SA226-T... AD, contact M7 Aerospace, LC, 10823 NE. Entrance Road, San Antonio, Texas 78216; telephone (210) 824...

  17. Microbial profiling of cpn60 universal target sequences in artificial mixtures of vaginal bacteria sampled by nylon swabs or self-sampling devices under different storage conditions.

    Science.gov (United States)

    Schellenberg, John J; Oh, Angela Yena; Hill, Janet E

    2017-05-01

    The vaginal microbiome is increasingly characterized by deep sequencing of universal genes. However, there are relatively few studies of how different specimen collection and sample storage and processing influence these molecular profiles. Here, we evaluate molecular microbial community profiles of samples collected using the HerSwab™ self-sampling device, compared to nylon swabs and under different storage conditions. In order to minimize technical variation, mixtures of 11 common vaginal bacteria in simulated vaginal fluid medium were sampled and DNA extracts prepared for massively parallel sequencing of the cpn60 universal target (UT). Three artificial mixtures imitating commonly observed vaginal microbiome profiles were easily distinguished and proportion of sequence reads correlated with the estimated proportion of the organism added to the artificial mixtures. Our results indicate that cpn60 UT amplicon sequencing quantifies the proportional abundance of member organisms in these artificial communities regardless of swab type or storage conditions, although some significant differences were observed between samples that were stored frozen and thawed prior to DNA extraction, compared to extractions from samples stored at room temperature for up to 7days. Our results indicate that an on-the-market device developed for infectious disease diagnostics may be appropriate for vaginal microbiome profiling, an approach that is increasingly facilitated by rapidly dropping deep sequencing costs. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Risk Profile in a Sample of Patients with Breast Cancer from the Public Health Perspective

    Directory of Open Access Journals (Sweden)

    Sorina IRIMIE

    2010-12-01

    Full Text Available Cancer represents a major public health and economical burden in developed countries and has emerged as a major public health problem in developing countries, matching its effect in industrialized nations. Although there have been recent declines in breast cancer mortality rates in some European Union countries, breast cancer remains of key importance to public health in Europe. Now days there is increasing recognition of the causative role of lifestyle factors, as smoking, diet, alcohol consumption, or lake of physical activity. The present study aimed to appreciate the presence and magnitude of modifiable risk factors for breast cancer in a sample of patients diagnosed with the disease, and to outline a risk profile liable to be changed in the intention of reducing the global risk. Risk factors have been investigated in 65 patients diagnosed with breast cancer using a questionnaire for breast cancer risk factors evaluation. The high risk profile was identified as taking shape for urban environment, modulated by the impact of overweight-obesity, smoking, reproductive factors and environmental exposure to different chemical substances. From the public health perspective, the control of overweight and obesity comes out in the foreground of preventive activities. Public health approaches emphasize on inexpensive, practical methods and in this perspective the approach of obesity should focus on the alteration of environmental context, promoting healthy eating and increased physical activity which could have a positive, independent impact on breast cancer risk

  19. Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay

    Directory of Open Access Journals (Sweden)

    Klotzle Brandy

    2011-08-01

    Full Text Available Abstract Background Microarray-based gene expression analysis of peripheral whole blood is a common strategy in the development of clinically relevant biomarker panels for a variety of human diseases. However, the results of such an analysis are often plagued by decreased sensitivity and reliability due to the effects of relatively high levels of globin mRNA in whole blood. Globin reduction assays have been shown to overcome such effects, but they require large amounts of total RNA and may induce distinct gene expression profiles. The Illumina whole genome DASL assay can detect gene expression levels using partially degraded RNA samples and has the potential to detect rare transcripts present in highly heterogeneous whole blood samples without the need for globin reduction. We assessed the utility of the whole genome DASL assay in an analysis of peripheral whole blood gene expression profiles. Results We find that gene expression detection is significantly increased with the use of whole genome DASL compared to the standard IVT-based direct hybridization. Additionally, globin-probe negative whole genome DASL did not exhibit significant improvements over globin-probe positive whole genome DASL. Globin reduction further increases the detection sensitivity and reliability of both whole genome DASL and IVT-based direct hybridization with little effect on raw intensity correlations. Raw intensity correlations between total RNA and globin reduced RNA were 0.955 for IVT-based direct hybridization and 0.979 for whole genome DASL. Conclusions Overall, the detection sensitivity of the whole genome DASL assay is higher than the IVT-based direct hybridization assay, with or without globin reduction, and should be considered in conjunction with globin reduction methods for future blood-based gene expression studies.

  20. Deteksi Kanker Kulit Melanoma dengan Linear Discriminant Analysis-Fuzzy k-Nearest Neigbhour Lp-Norm

    Directory of Open Access Journals (Sweden)

    Mustika Mentari

    2016-02-01

    Full Text Available Abstrak Seiring perkembangan teknologi dilakukan otomatisasi deteksi kanker kulit melalui citra dermoscopy. Pengambilan informasi fitur citra dermoscopy terganggu dengan outlier dan overfitting, karena faktor jenis kulit, penyebaran kanker yang tidak merata atau kesalahan sampling. Penelitian ini mengusulkan deteksi kanker kulit melanoma dengan mengintegrasikan metode fuzzy K-Nearest Neighbour (FuzzykNN, Lp-norm dan Linear Discriminant Analysis (LDA untuk mengurangi outlier dan overfitting. Masukan berupa citra warna RGB yang dinormalisasi menjadi RGBr. Reduksi dimensi dengan LDA menghasilkan fitur dengan nilai eigen paling menonjol. LDA pada penelitian ini menghasilkan dua fitur paling menonjol dari 141 jenis fitur, yaitu wilayah tumor dan minimum wilayah tumor channel R. Kemudian dilakukan klasifikasi FuzzykNN dan metode pengukur jarak Lp-norm. Penggunaan metode LDA dan Lp-norm dalam proses klasifikasi ini mengatasi terjadinya overfitting. Akurasi yang dihasilkan metode LDA-fuzzykNN Lp Norm, yaitu 72% saat masing-masing nilai p dan k = 25. Metode gabungan ini terbukti cukup baik dari pada metode yang dijalankan terpisah. Kata kunci: melanoma, fuzzy, KNN, Lp-norm, LDA. Abstract As the advancement of technology skin cancer detection need to be automated with the use of dermoscopy image. Outlier and overfitting are the problem in feature extraction of dermoscopy image, this can be caused by skin type, uneven cancer distribution or sampling error. This study proposed melanoma skin cancer detection by fuzzy K-Nearest Neighbour (FuzzykNN with Lp-norm integrated with Linear Discriminant Analysis (LDA to reduce the problem of outlier and overfitting. Input used in this study are images with RGB channel, then it adapted to RGBr. Dimensional reduction with LDA result in features with highest eigen value. LDA in this research select 2 discriminant, they are tumor area and minimum tumor area in R channel. This features then classified by fuzzykNN with Lp

  1. Concomitant effects of Ramadan fasting and time-of-day on apolipoprotein AI, B, Lp-a and homocysteine responses during aerobic exercise in Tunisian soccer players.

    Directory of Open Access Journals (Sweden)

    Omar Hammouda

    Full Text Available OBJECTIVE: To examine the time-of-day and Ramadan fasting (RF effects on serum apolipoprotein-AI (Apo-AI and B (Apo-B, lipoprotein particles-a (Lp-a, high-sensitive C-reactive-protein (hs-CRP, and homocysteine (Hcy during the Yo-Yo intermittent recovery test (YYIRT. DESIGN: Performance and biochemical measures were completed at two times-of-day (07:00 and 17:00 h, 1-week before RF (BR, the second week of RF (SWR, and the fourth week of RF (ER. SETTING: For each session, subjects performed the YYIRT, and blood samples were taken before and 3-min after the test for biochemical measures. PARTICIPANTS: Fifteen soccer players. MAIN OUTCOME MEASURES: Total distance during the YYIRT, core temperature, body composition, dietary intakes, lipid (HDL-C, LDL-C, Apo-AI, B and Lp-a and inflammatory (hs-CRP and Hcy profiles. RESULTS: Performances during the YYIRT were higher in the evening than the morning BR (P < 0.05, but this fluctuation was not observed during RF. Moreover, LDL-C, ApoB, and Lp-a were stable throughout the daytime BR. However, during RF, they decreased at 17:00 h (P < 0.05. Likewise, HDL-C and Apo-AI increased after the exercise and were higher at 17:00 h BR (P < 0.001. Moreover, these parameters increased during RF (P < 0.01. Furthermore, Hcy and hs-CRP increased during the exercise (P < 0.01 with higher evening levels BR. During ER, the diurnal pattern of Hcy was inversed (P < 0.001. CONCLUSIONS: This study concluded that caloric restriction induced by RF seems to ameliorate lipid and inflammatory markers of cardiovascular health during intermittent exercise performed in the evening.

  2. An uncooked vegan diet shifts the profile of human fecal microflora: computerized analysis of direct stool sample gas-liquid chromatography profiles of bacterial cellular fatty acids.

    Science.gov (United States)

    Peltonen, R; Ling, W H; Hänninen, O; Eerola, E

    1992-01-01

    The effect of an uncooked extreme vegan diet on fecal microflora was studied by direct stool sample gas-liquid chromatography (GLC) of bacterial cellular fatty acids and by quantitative bacterial culture by using classical microbiological techniques of isolation, identification, and enumeration of different bacterial species. Eighteen volunteers were divided randomly into two groups. The test group received an uncooked vegan diet for 1 month and a conventional diet of mixed Western type for the other month of the study. The control group consumed a conventional diet throughout the study period. Stool samples were collected. Bacterial cellular fatty acids were extracted directly from the stool samples and measured by GLC. Computerized analysis of the resulting fatty acid profiles was performed. Such a profile represents all bacterial cellular fatty acids in a sample and thus reflects its microflora and can be used to detect changes, differences, or similarities of bacterial flora between individual samples or sample groups. GLC profiles changed significantly in the test group after the induction and discontinuation of the vegan diet but not in the control group at any time, whereas quantitative bacterial culture did not detect any significant change in fecal bacteriology in either of the groups. The results suggest that an uncooked extreme vegan diet alters the fecal bacterial flora significantly when it is measured by direct stool sample GLC of bacterial fatty acids. PMID:1482187

  3. Child abuse and neglect profiles and their psychosocial consequences in a large sample of incarcerated males.

    Science.gov (United States)

    Debowska, Agata; Boduszek, Daniel

    2017-03-01

    Research examining child abuse and neglect (CAN) profiles among adult offender populations is lacking. Therefore, the primary aim of the present study was to address this limitation by using latent class analysis (LCA) to identify meaningful classes of individuals who have experienced physical abuse, emotional abuse, sexual abuse, as well as neglect. Another aim was to estimate the association between CAN class membership and external criteria (psychopathy factors, self-esteem, attitudes towards male sexual violence in dating relationships, child sexual abuse myth acceptance, violent offending, and age). Data were collected among a large systematically selected sample of adult male inmates (N=1261). Based on LCA, three unique classes of CAN were distinguished, including a 'low abuse' group (43.4% of the sample), a 'high physical and emotional abuse' group (51.3%), and a 'poly-victimized' group (5.3%). The analysis revealed that the CAN classes were differentially associated with affective responsiveness, cognitive responsiveness, personal self-esteem, prison self-esteem, attitudes towards male sexual violence in dating relationships, and violent offending. Findings highlight the unique nature of CAN constellations among criminal justice involved participants. The significance of the present results is discussed in relation to past and future research. Potential contributions to treatment strategies are also presented. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. Whole genome transcript profiling from fingerstick blood samples: a comparison and feasibility study

    Directory of Open Access Journals (Sweden)

    Williams Adam R

    2009-12-01

    Full Text Available Abstract Background Whole genome gene expression profiling has revolutionized research in the past decade especially with the advent of microarrays. Recently, there have been significant improvements in whole blood RNA isolation techniques which, through stabilization of RNA at the time of sample collection, avoid bias and artifacts introduced during sample handling. Despite these improvements, current human whole blood RNA stabilization/isolation kits are limited by the requirement of a venous blood sample of at least 2.5 mL. While fingerstick blood collection has been used for many different assays, there has yet to be a kit developed to isolate high quality RNA for use in gene expression studies from such small human samples. The clinical and field testing advantages of obtaining reliable and reproducible gene expression data from a fingerstick are many; it is less invasive, time saving, more mobile, and eliminates the need of a trained phlebotomist. Furthermore, this method could also be employed in small animal studies, i.e. mice, where larger sample collections often require sacrificing the animal. In this study, we offer a rapid and simple method to extract sufficient amounts of high quality total RNA from approximately 70 μl of whole blood collected via a fingerstick using a modified protocol of the commercially available Qiagen PAXgene RNA Blood Kit. Results From two sets of fingerstick collections, about 70 uL whole blood collected via finger lancet and capillary tube, we recovered an average of 252.6 ng total RNA with an average RIN of 9.3. The post-amplification yields for 50 ng of total RNA averaged at 7.0 ug cDNA. The cDNA hybridized to Affymetrix HG-U133 Plus 2.0 GeneChips had an average % Present call of 52.5%. Both fingerstick collections were highly correlated with r2 values ranging from 0.94 to 0.97. Similarly both fingerstick collections were highly correlated to the venous collection with r2 values ranging from 0.88 to 0

  5. Serum Protein Profile Study of Clinical Samples Using High Performance Liquid Chromatography-Laser Induced Fluorescence

    DEFF Research Database (Denmark)

    Karemore, Gopal Raghunath; Ukendt, Sujatha; Rai, Lavanya

    2009-01-01

    The serum protein profiles of normal subjects, patients diagnosed with cervical cancer, and oral cancer were recorded using High Performance Liquid Chromatography combined with Laser Induced Fluorescence detection (HPLC-LIF). Serum protein profiles of the above three classes were tested...... for establishing the ability of HPLC-LIF protein profiling technique for discrimination, using hard clustering and Fuzzy clustering methods. The clustering algorithms have quite successfully classified the profiles as belonging to normal, cancer of cervix, and oral cancer conditions....

  6. Inferring predominant pathways in cellular models of breast cancer using limited sample proteomic profiling

    Directory of Open Access Journals (Sweden)

    Klinke David J

    2010-06-01

    Full Text Available Abstract Background Molecularly targeted drugs inhibit aberrant signaling within oncogenic pathways. Identifying the predominant pathways at work within a tumor is a key step towards tailoring therapies to the patient. Clinical samples pose significant challenges for proteomic profiling, an attractive approach for identifying predominant pathways. The objective of this study was to determine if information obtained from a limited sample (i.e., a single gel replicate can provide insight into the predominant pathways in two well-characterized breast cancer models. Methods A comparative proteomic analysis of total cell lysates was obtained from two cellular models of breast cancer, BT474 (HER2+/ER+ and SKBR3 (HER2+/ER-, using two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Protein interaction networks and canonical pathways were extracted from the Ingenuity Pathway Knowledgebase (IPK based on association with the observed pattern of differentially expressed proteins. Results Of the 304 spots that were picked, 167 protein spots were identified. A threshold of 1.5-fold was used to select 62 proteins used in the analysis. IPK analysis suggested that metabolic pathways were highly associated with protein expression in SKBR3 cells while cell motility pathways were highly associated with BT474 cells. Inferred protein networks were confirmed by observing an up-regulation of IGF-1R and profilin in BT474 and up-regulation of Ras and enolase in SKBR3 using western blot. Conclusion When interpreted in the context of prior information, our results suggest that the overall patterns of differential protein expression obtained from limited samples can still aid in clinical decision making by providing an estimate of the predominant pathways that underpin cellular phenotype.

  7. Contamination profiles of short-chain polychlorinated n-alkanes in foodstuff samples from Japan

    Energy Technology Data Exchange (ETDEWEB)

    Matsukami, Hidenori; Kurunthachalam, S.; Ohi, Etsumasa; Takasuga, Takumi [Shimadzu Techno Research, Inc., Kyoto (Japan); Iino, Fukuya; Nakanishi, Junko [National Inst. of Advanced Industrial Science and Technology, Tsukuba (Japan)

    2004-09-15

    Polychlorinated n-alkanes (PCAs) are group of chemicals manufactured by chlorination of liquid n-paraffin or paraffin wax that contain 30 to 70% chlorine by weight. Large amounts of PCAs are widely used as plasticizers for vinyl chloride, lubricants, paints, and flame retardants and number of other industrial applications. Annual global production of PCAs is approximately 300 kilo tones, with a majority having medium-carbon-chain (C14-C19) length. According to the investigation made by Kagaku Kogyo Nippon-Sha, the annual consumption of PCAs in Japan was about 83,000 tons in between 1986-2001. Short-carbon-chain (C10-C13) has been placed on the Priority Substance List under Canadian Environmental Protection Act and on the Environmental Protection Agency Toxic Release Inventory in the USA due to its potential to act as tumor promoters in mammals. Data on environment levels of PCAs is meager, nevertheless, PCAs have been measured at relatively high concentrations in biota from Sweden, biota, sediment from Canada and marine biota and human milk from the Canadian Arctic. In our earlier study, we reported concentrations of short-chain PCAs from sewage treatment plant (STP) collected from Tama River, Tokyo and river water and sediment from Tokyo and Osaka. STP influent water contained greater shortchain PCAs concentrations than STP effluent. In addition, some river water and sediment samples contained detectable concentrations of short-chain PCAs, which was similar to other industrial countries. However, there is no study conducted to explore the contamination profiles of short-chain PCAs in human foodstuff samples. In the present study, we analyzed eleven foodstuff samples that were purchased from various supermarkets in order to know the short-chain PCAs concentrations in the foodstuff and possible human total daily intake (TDI) amounts.

  8. Profiling the clinical presentation of diagnostic characteristics of a sample of symptomatic TMD patients

    Directory of Open Access Journals (Sweden)

    e Silva Machado Luciana

    2012-08-01

    Full Text Available Abstract Background Temporomandibular disorder (TMD patients might present a number of concurrent clinical diagnoses that may be clustered according to their similarity. Profiling patients’ clinical presentations can be useful for better understanding the behavior of TMD and for providing appropriate treatment planning. The aim of this study was to simultaneously classify symptomatic patients diagnosed with a variety of subtypes of TMD into homogenous groups based on their clinical presentation and occurrence of comorbidities. Methods Clinical records of 357 consecutive TMD patients seeking treatment in a private specialized clinic were included in the study sample. Patients presenting multiple subtypes of TMD diagnosed simultaneously were categorized according to the AAOP criteria. Descriptive statistics and two-step cluster analysis were used to characterize the clinical presentation of these patients based on the primary and secondary clinical diagnoses. Results The most common diagnoses were localized masticatory muscle pain (n = 125 and disc displacement without reduction (n = 104. Comorbidity was identified in 288 patients. The automatic selection of an optimal number of clusters included 100% of cases, generating an initial 6-cluster solution and a final 4-cluster solution. The interpretation of within-group ranking of the importance of variables in the clustering solutions resulted in the following characterization of clusters: chronic facial pain (n = 36, acute muscle pain (n = 125, acute articular pain (n = 75 and chronic articular impairment (n = 121. Conclusion Subgroups of acute and chronic TMD patients seeking treatment can be identified using clustering methods to provide a better understanding of the clinical presentation of TMD when multiple diagnosis are present. Classifying patients into identifiable symptomatic profiles would help clinicians to estimate how common a disorder is within a population of

  9. Gradient Compared Lp-LMS Algorithms for Sparse System Identification

    OpenAIRE

    Feng, Yong; Wu, Jiasong; Zeng, Rui; Luo, Limin; Shu, Huazhong

    2015-01-01

    In this paper, we propose two novel p-norm penalty least mean square (Lp-LMS) algorithms as supplements of the conventional Lp-LMS algorithm established for sparse adaptive filtering recently. A gradient comparator is employed to selectively apply the zero attractor of p-norm constraint for only those taps that have the same polarity as that of the gradient of the squared instantaneous error, which leads to the new proposed gradient compared p-norm constraint LMS algorithm (LpGC-LMS). We expl...

  10. Concomitant effects of Ramadan fasting and time-of-day on apolipoprotein AI, B, Lp-a and homocysteine responses during aerobic exercise in Tunisian soccer players.

    Science.gov (United States)

    Hammouda, Omar; Chtourou, Hamdi; Aloui, Asma; Chahed, Henda; Kallel, Choumous; Miled, Abdelhedi; Chamari, Karim; Chaouachi, Anis; Souissi, Nizar

    2013-01-01

    To examine the time-of-day and Ramadan fasting (RF) effects on serum apolipoprotein-AI (Apo-AI) and B (Apo-B), lipoprotein particles-a (Lp-a), high-sensitive C-reactive-protein (hs-CRP), and homocysteine (Hcy) during the Yo-Yo intermittent recovery test (YYIRT). Performance and biochemical measures were completed at two times-of-day (07:00 and 17:00 h), 1-week before RF (BR), the second week of RF (SWR), and the fourth week of RF (ER). For each session, subjects performed the YYIRT, and blood samples were taken before and 3-min after the test for biochemical measures. Fifteen soccer players. Total distance during the YYIRT, core temperature, body composition, dietary intakes, lipid (HDL-C, LDL-C, Apo-AI, B and Lp-a) and inflammatory (hs-CRP and Hcy) profiles. Performances during the YYIRT were higher in the evening than the morning BR (P exercise and were higher at 17:00 h BR (P exercise (P evening levels BR. During ER, the diurnal pattern of Hcy was inversed (P exercise performed in the evening.

  11. Relative power and sample size analysis on gene expression profiling data

    Directory of Open Access Journals (Sweden)

    den Dunnen JT

    2009-09-01

    Full Text Available Abstract Background With the increasing number of expression profiling technologies, researchers today are confronted with choosing the technology that has sufficient power with minimal sample size, in order to reduce cost and time. These depend on data variability, partly determined by sample type, preparation and processing. Objective measures that help experimental design, given own pilot data, are thus fundamental. Results Relative power and sample size analysis were performed on two distinct data sets. The first set consisted of Affymetrix array data derived from a nutrigenomics experiment in which weak, intermediate and strong PPARα agonists were administered to wild-type and PPARα-null mice. Our analysis confirms the hierarchy of PPARα-activating compounds previously reported and the general idea that larger effect sizes positively contribute to the average power of the experiment. A simulation experiment was performed that mimicked the effect sizes seen in the first data set. The relative power was predicted but the estimates were slightly conservative. The second, more challenging, data set describes a microarray platform comparison study using hippocampal δC-doublecortin-like kinase transgenic mice that were compared to wild-type mice, which was combined with results from Solexa/Illumina deep sequencing runs. As expected, the choice of technology greatly influences the performance of the experiment. Solexa/Illumina deep sequencing has the highest overall power followed by the microarray platforms Agilent and Affymetrix. Interestingly, Solexa/Illumina deep sequencing displays comparable power across all intensity ranges, in contrast with microarray platforms that have decreased power in the low intensity range due to background noise. This means that deep sequencing technology is especially more powerful in detecting differences in the low intensity range, compared to microarray platforms. Conclusion Power and sample size analysis

  12. Relative power and sample size analysis on gene expression profiling data

    Science.gov (United States)

    van Iterson, M; 't Hoen, PAC; Pedotti, P; Hooiveld, GJEJ; den Dunnen, JT; van Ommen, GJB; Boer, JM; Menezes, RX

    2009-01-01

    Background With the increasing number of expression profiling technologies, researchers today are confronted with choosing the technology that has sufficient power with minimal sample size, in order to reduce cost and time. These depend on data variability, partly determined by sample type, preparation and processing. Objective measures that help experimental design, given own pilot data, are thus fundamental. Results Relative power and sample size analysis were performed on two distinct data sets. The first set consisted of Affymetrix array data derived from a nutrigenomics experiment in which weak, intermediate and strong PPARα agonists were administered to wild-type and PPARα-null mice. Our analysis confirms the hierarchy of PPARα-activating compounds previously reported and the general idea that larger effect sizes positively contribute to the average power of the experiment. A simulation experiment was performed that mimicked the effect sizes seen in the first data set. The relative power was predicted but the estimates were slightly conservative. The second, more challenging, data set describes a microarray platform comparison study using hippocampal δC-doublecortin-like kinase transgenic mice that were compared to wild-type mice, which was combined with results from Solexa/Illumina deep sequencing runs. As expected, the choice of technology greatly influences the performance of the experiment. Solexa/Illumina deep sequencing has the highest overall power followed by the microarray platforms Agilent and Affymetrix. Interestingly, Solexa/Illumina deep sequencing displays comparable power across all intensity ranges, in contrast with microarray platforms that have decreased power in the low intensity range due to background noise. This means that deep sequencing technology is especially more powerful in detecting differences in the low intensity range, compared to microarray platforms. Conclusion Power and sample size analysis based on pilot data give

  13. AppLP: A Dialogue on Applications of Logic Programming

    OpenAIRE

    Warren, David S.; Liu, Yanhong A.

    2017-01-01

    This document describes the contributions of the 2016 Applications of Logic Programming Workshop (AppLP), which was held on October 17 and associated with the International Conference on Logic Programming (ICLP) in Flushing, New York City.

  14. LP formulation of asymmetric zero-sum stochastic games

    KAUST Repository

    Li, Lichun

    2014-12-15

    This paper provides an efficient linear programming (LP) formulation of asymmetric two player zero-sum stochastic games with finite horizon. In these stochastic games, only one player is informed of the state at each stage, and the transition law is only controlled by the informed player. Compared with the LP formulation of extensive stochastic games whose size grows polynomially with respect to the size of the state and the size of the uninformed player\\'s actions, our proposed LP formulation has its size to be linear with respect to the size of the state and the size of the uninformed player, and hence greatly reduces the computational complexity. A travelling inspector problem is used to demonstrate the efficiency of the proposed LP formulation.

  15. Influence of common preanalytical variations on the metabolic profile of serum samples in biobanks

    Energy Technology Data Exchange (ETDEWEB)

    Fliniaux, Ophelie [University of Picardie Jules Verne, Laboratoire de Phytotechnologie EA 3900-BioPI (France); Gaillard, Gwenaelle [Biobanque de Picardie (France); Lion, Antoine [University of Picardie Jules Verne, Laboratoire de Phytotechnologie EA 3900-BioPI (France); Cailleu, Dominique [Batiment Serres-Transfert, rue de Mai/rue Dallery, Plateforme Analytique (France); Mesnard, Francois, E-mail: francois.mesnard@u-picardie.fr [University of Picardie Jules Verne, Laboratoire de Phytotechnologie EA 3900-BioPI (France); Betsou, Fotini [Integrated Biobank of Luxembourg (Luxembourg)

    2011-12-15

    A blood pre-centrifugation delay of 24 h at room temperature influenced the proton NMR spectroscopic profiles of human serum. A blood pre-centrifugation delay of 24 h at 4 Degree-Sign C did not influence the spectroscopic profile as compared with 4 h delays at either room temperature or 4 Degree-Sign C. Five or ten serum freeze-thaw cycles also influenced the proton NMR spectroscopic profiles. Certain common in vitro preanalytical variations occurring in biobanks may impact the metabolic profile of human serum.

  16. Determination of DNA profiling of siwak and toothbrush samples used in Kingdom of Saudi Arabia

    Directory of Open Access Journals (Sweden)

    Nagy Alfadaly

    2016-10-01

    Conclusion: Siwak contains enough quantity of DNA, and retained good DNA profiling; and when compared to toothbrushes, siwak is a reasonable source of DNA profiling when found at the scene of crime. In addition, time of storage up to 4 months had no or little effects on results.

  17. Determining the group velocity dispersion by field analysis for the LP0X, LP1X, and LP2X mode groups independently of the fiber length: applications to step-index fibers

    DEFF Research Database (Denmark)

    Israelsen, Stine Møller; Usuga Castaneda, Mario A.; Rottwitt, Karsten

    2017-01-01

    By knowing the electric field distribution of a guided mode in an optical fiber, we are able to evaluate the group velocity dispersion in a weakly guiding step-index fiber for a pure mode in the LP0X, LP1X, and LP2X mode groups independently of the fiber length. We demonstrate the method...

  18. PERSONALITY PROFILES AND PROBLEMATIC INTERNET USE IN A SAMPLE OF ITALIAN ADOLESCENTS

    Directory of Open Access Journals (Sweden)

    Fanny Guglielmucci

    2017-02-01

    Full Text Available Objective: The aim of the present study was to investigate the relationship between specific personality profiles and Internet use in a sample of Italian adolescents. Method: Four hundred thirty-two adolescents (58.3% males with an average age of 14.41 years (SD=.95 were enrolled in the study. Participants were administered the Internet Addiction Test (IAT and the Minnesota Multiphasic Inventory – Adolescent Form (MMPI-A. A two-step cluster analysis was relied according to IAT items’ scores. Results: Participants were grouped into three clusters labeled “Regulated Internet users” (n=180, “Involved with Internet activities” (n=105, and “At risk for problematic Internet use” (n=147. Consistently, the group at-risk for problematic Internet use showed higher IAT score and MMPI-A scores than the other groups, while no differences emerged between the group of regulated Internet users and the group of those involved with Internet activities. For the group at risk for problematic Internet use, the MMPI-A Clinical Scales on Paranoia (Pa and Schizophrenia (Sc showed the highest elevation, indicating a MMPI-A codetype 6-8/8-6 which describes adolescents with ego immaturity, dysregulated affects and behaviors, and reduced reality testing. Conclusions: Adolescents at risk for developing a dysfunctional use of the Internet may have little insight, bizarre beliefs, grandiose thought, and a persecutory view of the external world that may limit their capacity to counteract feelings of hopelessness and anguish. They could perceive the Internet as safe environment where it is possible to express such dysregulated feelings and behaviors, and to cope with emotional distress.

  19. Microbial profile of a kefir sample preparations: grains in natura and lyophilized and fermented suspension

    Directory of Open Access Journals (Sweden)

    Rafaela Strada de Oliveira Bergmann

    2010-12-01

    Full Text Available Probiotics are supplementary foods developed by microbial strains that improve animal health beyond basic nutrition. Probiotics are consumed orally, regardless of being considered as normal inhabitants of the intestines, able to survive in enzimatic and biliary secretions. Kefir is a probiotic originated from the old continent, fermented by several bacteria and yeasts, encapsulated in a polyssacharide matrix, and resembles jelly grains. Kefir is also presented as its sourish product both in sugary or milky suspensions containing vitamins, aminoacids, peptides, carbohydrates, ethanol, and volatile compounds. Kefir is known to have a diverse microbial content depending on the country and fermentative substrates, which cause distinct probiotic effects. In this sense, the purpose of this work was to isolate, identify, and quantify the microbial content of a native sugary kefir sample (fermented suspension and lyophilized natural grains. Serial dilutions were plated on Rogosa agar (AR and De Man, Rogosa and Sharpe (MRS, for Lactobacillus; Brain Heart Infusion (BHI, for total bacteria; Sabouraud-Dextrose-Agar (SDA, for yeasts and filamentous fungi; Thioglycolate Agar (TA, for Streptococcus, Acetobacteria and Leuconostoc; and Coconut Water Agar (CWA, and CWA supplemented with yeast extract (CWAY, for various genera. Genera and species for all strains were identified through biochemical reactions and specific API systems. The microbial profile of kefir was different from other sources of grains despite the presence of similar microorganisms and others which have not been reported yet. The data obtained with the CWA and CWAE media suggest that both substrates are alternative and salutary media for culture of kefir strains.

  20. Variation in LPA is associated with Lp(a levels in three populations from the Third National Health and Nutrition Examination Survey.

    Directory of Open Access Journals (Sweden)

    Logan Dumitrescu

    2011-01-01

    Full Text Available The distribution of lipoprotein(a [Lp(a] levels can differ dramatically across diverse racial/ethnic populations. The extent to which genetic variation in LPA can explain these differences is not fully understood. To explore this, 19 LPA tagSNPs were genotyped in 7,159 participants from the Third National Health and Nutrition Examination Survey (NHANES III. NHANES III is a diverse population-based survey with DNA samples linked to hundreds of quantitative traits, including serum Lp(a. Tests of association between LPA variants and transformed Lp(a levels were performed across the three different NHANES subpopulations (non-Hispanic whites, non-Hispanic blacks, and Mexican Americans. At a significance threshold of p<0.0001, 15 of the 19 SNPs tested were strongly associated with Lp(a levels in at least one subpopulation, six in at least two subpopulations, and none in all three subpopulations. In non-Hispanic whites, three variants were associated with Lp(a levels, including previously known rs6919246 (p = 1.18 × 10(-30. Additionally, 12 and 6 variants had significant associations in non-Hispanic blacks and Mexican Americans, respectively. The additive effects of these associated alleles explained up to 11% of the variance observed for Lp(a levels in the different racial/ethnic populations. The findings reported here replicate previous candidate gene and genome-wide association studies for Lp(a levels in European-descent populations and extend these findings to other populations. While we demonstrate that LPA is an important contributor to Lp(a levels regardless of race/ethnicity, the lack of generalization of associations across all subpopulations suggests that specific LPA variants may be contributing to the observed Lp(a between-population variance.

  1. Dose-Response Analysis of RNA-Seq Profiles in Archival Formalin-Fixed Paraffin-Embedded (FFPE) Samples.

    Science.gov (United States)

    Use of archival resources has been limited to date by inconsistent methods for genomic profiling of degraded RNA from formalin-fixed paraffin-embedded (FFPE) samples. RNA-sequencing offers a promising way to address this problem. Here we evaluated transcriptomic dose responses us...

  2. Proteomic Profiling of Paraffin-Embedded Samples Identifies Metaplasia-Specific and Early-Stage Gastric Cancer Biomarkers

    Science.gov (United States)

    Sousa, Josane F.; Ham, Amy-Joan L.; Whitwell, Corbin; Nam, Ki Taek; Lee, Hyuk-Joon; Yang, Han-Kwang; Kim, Woo Ho; Zhang, Bing; Li, Ming; LaFleur, Bonnie; Liebler, Daniel C.; Goldenring, James R.

    2013-01-01

    Early diagnosis and curative resection are the predominant factors associated with increased survival in patients with gastric cancer. However, most gastric cancer cases are still diagnosed at later stages. Since most pathologic specimens are archived as FFPE samples, the ability to use them to generate expression profiles can greatly improve cancer biomarker discovery. We sought to uncover new biomarkers for stomach preneoplastic metaplasias and neoplastic lesions by generating proteome profiles using FFPE samples. We combined peptide isoelectric focusing and liquid chromatography–tandem mass spectrometry analysis to generate proteomic profiles from FFPE samples of intestinal-type gastric cancer, metaplasia, and normal mucosa. The expression patterns of selected proteins were analyzed by immunostaining first in single tissue sections from normal stomach, metaplasia, and gastric cancer and later in larger tissue array cohorts. We detected 60 proteins up-regulated and 87 proteins down-regulated during the progression from normal mucosa to metaplasia to gastric cancer. Two of the up-regulated proteins, LTF and DMBT1, were validated as specific markers for spasmolytic polypeptide–expressing metaplasia and intestinal metaplasia, respectively. In cancers, significantly lower levels of DMBT1 or LTF correlated with more advanced disease and worse prognosis. Thus, proteomic profiling using FFPE samples has led to the identification of two novel markers for stomach metaplasias and gastric cancer prognosis. PMID:22944598

  3. Bacterial community profiling of cryogenically ground samples from the apical and coronal root segments of teeth with apical periodontitis.

    Science.gov (United States)

    Alves, Flávio R F; Siqueira, José F; Carmo, Flávia L; Santos, Adriana L; Peixoto, Raquel S; Rôças, Isabela N; Rosado, Alexandre S

    2009-04-01

    Bacteria located at the apical part of infected root canals are arguably directly involved in the pathogenesis of apical periodontitis. This study was conducted to profile and further compare the bacterial communities established at the apical and middle/coronal segments of infected root canals. Extracted teeth with attached apical periodontitis lesions were sectioned so as to obtain two root fragments representing the apical third and the coronal two thirds. Root fragments were subjected to a cryogenic grinding approach. DNA was extracted from root powder samples and used as a template for bacterial community profiling using a 16S ribosomal RNA gene-based seminested polymerase chain reaction/denaturing gradient gel electrophoresis approach. The mean number of bands in apical samples from teeth with primary infections was 28, ranging from 18 to 48, whereas in the middle/coronal samples, it was also 28, ranging from 19 to 36. Findings showed that the profile of bacterial community colonizing the apical third of infected root canals is as diverse as that occurring at the middle/coronal thirds. A high variability was observed for both interindividual (samples from the same region but from different patients) and intraindividual (samples from different regions of the same tooth) comparisons. The methodology used to prepare and analyze samples was highly effective in disclosing a previously unanticipated broad diversity of endodontic bacterial communities, especially at the apical part of infected root canals.

  4. Sparse Adaptive Channel Estimation Based on lp-Norm-Penalized Affine Projection Algorithm

    OpenAIRE

    Yingsong Li; Wenxing Li; Wenhua Yu; Jian Wan; Zhiwei Li

    2014-01-01

    We propose an lp-norm-penalized affine projection algorithm (LP-APA) for broadband multipath adaptive channel estimations. The proposed LP-APA is realized by incorporating an lp-norm into the cost function of the conventional affine projection algorithm (APA) to exploit the sparsity property of the broadband wireless multipath channel, by which the convergence speed and steady-state performance of the APA are significantly improved. The implementation of the LP-APA is equivalent to adding a z...

  5. The Harter Self-Perception Profile for Adolescents: Psychometrics for an Early Adolescent, African American Sample.

    Science.gov (United States)

    Thomson, Nicole Renick; Zand, Debra H.

    2002-01-01

    Studied the factor structure and other psychometric properties of S. Harter's (1988) Self-Perception Profile for Adolescents for 174 African American early adolescents. Findings yielded six conceptually relevant factors and also indicated gender differences in several domains. (SLD)

  6. Comparison of DNA extraction methods for microbial community profiling with an application to pediatric bronchoalveolar lavage samples.

    Directory of Open Access Journals (Sweden)

    Dana Willner

    Full Text Available Barcoded amplicon sequencing is rapidly becoming a standard method for profiling microbial communities, including the human respiratory microbiome. While this approach has less bias than standard cultivation, several steps can introduce variation including the type of DNA extraction method used. Here we assessed five different extraction methods on pediatric bronchoalveolar lavage (BAL samples and a mock community comprised of nine bacterial genera to determine method reproducibility and detection limits for these typically low complexity communities. Additionally, using the mock community, we were able to evaluate contamination and select a relative abundance cut-off threshold based on the geometric distribution that optimizes the trade off between detecting bona fide operational taxonomic units and filtering out spurious ones. Using this threshold, the majority of genera in the mock community were predictably detected by all extraction methods including the hard-to-lyse Gram-positive genus Staphylococcus. Differences between extraction methods were significantly greater than between technical replicates for both the mock community and BAL samples emphasizing the importance of using a standardized methodology for microbiome studies. However, regardless of method used, individual patients retained unique diagnostic profiles. Furthermore, despite being stored as raw frozen samples for over five years, community profiles from BAL samples were consistent with historical culturing results. The culture-independent profiling of these samples also identified a number of anaerobic genera that are gaining acceptance as being part of the respiratory microbiome. This study should help guide researchers to formulate sampling, extraction and analysis strategies for respiratory and other human microbiome samples.

  7. Characterization of human high-density lipoprotein subclasses LP A-I and LP A-I/A-II and binding to HepG2 cells

    NARCIS (Netherlands)

    E.P.C. Kilsdonk (Liesbeth); T. van Gent (Teus); A. van Tol (Arie)

    1990-01-01

    markdownabstractAbstract Plasma HDL can be classified according to their apolipoprotein content into at least two types of lipoprotein particles: lipoproteins containing both apo A-I and apo A-II (LP A-I/A-II) and lipoproteins with apo A-I but without apo A-II (LP A-I). LP A-I and LP A-I/A-II

  8. Increased risk of coronary artery calcification progression in subjects with high baseline Lp(a) levels: The Kangbuk Samsung Health Study.

    Science.gov (United States)

    Cho, Jung Hwan; Lee, Da Young; Lee, Eun Seo; Kim, Jihyun; Park, Se Eun; Park, Cheol-Young; Lee, Won-Young; Oh, Ki-Won; Park, Sung-Woo; Rhee, Eun-Jung

    2016-11-01

    Results from previous studies support the association of lipoprotein(a) [Lp(a)] levels and coronary artery disease risk. In this study, we analyzed the association between baseline Lp(a) levels and future progression of coronary artery calcification (CAC) in apparently healthy Korean adults. A total of 2611 participants (mean age: 41years, 92% mend) who underwent a routine health check-up in 2010 and 2014 were enrolled. Coronary artery calcium score (CACS) were measured by multi-detector computed tomography. Baseline Lp(a) was measured by high-sensitivity immunoturbidimetric assay. Progression of CAC was defined as a change in CACS >0 over four years. Bivariate correlation analyses with baseline Lp(a) and other metabolic parameters revealed age, total cholesterol, HDL-C, LDL-C and CACS to have a significant positive correlation, while body weight, fasting glucose level, blood pressure and triglyceride level were negatively correlated with baseline Lp(a) level. After four years of follow-up, 635 subjects (24.3%) had CAC progression. The participants who had CAC progression were older, composed of more men, more obese, and had higher fasting glucose levels and worse baseline lipid profiles compared to those who did not have CAC progression. The mean serum Lp(a) level was significantly higher in subjects who had CAC progression compared to those who did not (32.5 vs. 28.9mg/dL, p<0.01). When the risk for CAC progression according to baseline Lp(a) was calculated, those with Lp(a) level≥50mg/dL had an odds ratio of 1.333 (95% CI 1.027-1.730) for CAC progression compared to those with Lp(a)<50mg/dL after adjusting for confounding factors. In this study, the subjects who had higher Lp(a) were at significantly higher risk for CAC progression after four years of follow-up, suggesting the role of high Lp(a) in CAC progression. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  9. Questionnaires suggest a small overall effect of physical activity level and stress control over men’s lipid profile

    Directory of Open Access Journals (Sweden)

    Maria Fátima Glaner

    2013-07-01

    Full Text Available This study aimed to verify whether lipid profile (LP differs between men classified into two categories of physical activity level (PAL and stress control; and to analyze the results according to the evidence that suggests an inverse relationship among PAL, LP and stress control. The sample was composed of 109 subjects (age = 33.7±8.0 years. To evaluate the PAL we used the “Habitual Physical Activity Questionnaire”. To evaluate stress control, we used the “Profile of Individual Lifestyle” questionnaire, classifying subjects into one of the following categories: “bad PAL” or “good PAL”, “yes stress control” or “no stress control”. We obtained the LP using an enzymatic spectrophotometric method. Analysis of variance with two factors (p 0.05 between men in the “bad PAL” / “good PAL” groups and in the “yes stress control” / “no stress control” groups. There was a small chance (p > 0.05 for PAL and stress control to have an influence on LP, ranging from 14.5 (VLDL to 23.7% (LDL for PAL and from 5.1 (LDL to 10.9% (VLDL for stress control. These factors hardly explained (p > 0.05 the LP variation, which peaked at 1.7%. When under the same circumstances of this study, the overall effect of PAL and stress control may apply to all LP. These findings have important implications for studies that aim to verify whether the subjects reached “good PAL”. We suppose that these findings are due to a possible inaccuracy in the PAL and stress control assessment.

  10. How Confocal Is Confocal Raman Microspectroscopy on the Skin? Impact of Microscope Configuration and Sample Preparation on Penetration Depth Profiles.

    Science.gov (United States)

    Lunter, Dominique Jasmin

    2016-01-01

    The aim of the study was to elucidate the effect of sample preparation and microscope configuration on the results of confocal Raman microspectroscopic evaluation of the penetration of a pharmaceutical active into the skin (depth profiling). Pig ear skin and a hydrophilic formulation containing procaine HCl were used as a model system. The formulation was either left on the skin during the measurement, or was wiped off or washed off prior to the analysis. The microscope configuration was varied with respect to objectives and pinholes used. Sample preparation and microscope configuration had a tremendous effect on the results of depth profiling. Regarding sample preparation, the best results could be observed when the formulation was washed off the skin prior to the analysis. Concerning microscope configuration, the use of a 40 × 0.6 numerical aperture (NA) objective in combination with a 25-µm pinhole or a 100 × 1.25 NA objective in combination with a 50-µm pinhole was found to be advantageous. Complete removal of the sample from the skin before the analysis was found to be crucial. A thorough analysis of the suitability of the chosen microscope configuration should be performed before acquiring concentration depth profiles. © 2016 S. Karger AG, Basel.

  11. Profiles of Emotional Intelligence and Learning Strategies in a Sample of Chilean Students

    Science.gov (United States)

    García-Fernández, José M.; Inglés, Cándido J.; Suriá, Raquel; Martín, Nelly Lagos-San; Gonzálvez-Maciá, Carolina; Aparisi, David; Martínez-Monteagudo, Mari C.

    2015-01-01

    In the last few years, one of the lines of research of great interest in the field of emotional intelligence (EI) has been the analysis of the role of emotions in the educational context and, in particular, their influence on learning strategies. The aims of this study are to identify the existence of different EI profiles and to determine…

  12. The importance of Guthrie cards and other medical samples for the direct matching of disaster victims using DNA profiling.

    Science.gov (United States)

    Hartman, D; Benton, L; Morenos, L; Beyer, J; Spiden, M; Stock, A

    2011-02-25

    The identification of disaster victims through the use of DNA analysis is an integral part of any Disaster Victim Identification (DVI) response, regardless of the scale and nature of the disaster. As part of the DVI response to the 2009 Victorian Bushfires Disaster, DNA analysis was performed to assist in the identification of victims through kinship (familial matching to relatives) or direct (self source sample) matching of DNA profiles. Although most of the DNA identifications achieved were to reference samples from relatives, there were a number of DNA identifications (12) made through direct matching. Guthrie cards, which have been collected in Australia over the past 30 years, were used to provide direct reference samples. Of the 236 ante-mortem (AM) samples received, 21 were Guthrie cards and one was a biopsy specimen; all yielding complete DNA profiles when genotyped. This publication describes the use of such Biobanks and medical specimens as a sample source for the recovery of good quality DNA for comparisons to post-mortem (PM) samples. Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.

  13. A simple and sensitive method for lipoprotein and lipids profiles analysis of individual micro-liter scale serum samples.

    Science.gov (United States)

    Yang, Liu; Fan, Baoyan; Yang, Kangmin; Zhu, Haibo

    2012-02-01

    A simple and sensitive method to determine lipoprotein and lipids profiles in micro-liter scale individual serum sample is not presently available. Traditional lipoprotein separation techniques either by ultra-centrifugation or by liquid chromatography methods have their disadvantages in both lipoprotein separation and lipids component quantification. In this study we used small volume needing size-exclusion fast protein liquid chromatography to separate different lipoprotein subclasses in 50μL serum. And lipids contents, such as cholesterol, cholesterol ester and triacylglycerol, were measured by using two different fluorescence-based lipid detection methods. With this method, very low density lipoprotein, low density lipoprotein and high density lipoprotein could be easily separated, and follow-up lipid detection was completed by simple kinds of reactions. Serum lipoprotein and lipids profiling from C57BL/6 mice (n=5) and human (n=5) were analyzed. The elution profiles of five individuals were highly reproducible, and there were lipoprotein and lipids distribution variations between C57BL/6 mice and human beings. In conclusion, this method which combined small volume needing size-exclusion fast protein liquid chromatography and fluorescence-based lipids measurement, provided a simple, efficient, integrity and reproducible procedure for determining serum lipoprotein and lipids profiles in micro-liter scale levels. It becomes possible that determination of lipoprotein profiles and gaining information of lipids in different lipoproteins can be accomplished simultaneously. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  14. Sampling

    CERN Document Server

    Thompson, Steven K

    2012-01-01

    Praise for the Second Edition "This book has never had a competitor. It is the only book that takes a broad approach to sampling . . . any good personal statistics library should include a copy of this book." —Technometrics "Well-written . . . an excellent book on an important subject. Highly recommended." —Choice "An ideal reference for scientific researchers and other professionals who use sampling." —Zentralblatt Math Features new developments in the field combined with all aspects of obtaining, interpreting, and using sample data Sampling provides an up-to-date treat

  15. IHC Profiler: an open source plugin for the quantitative evaluation and automated scoring of immunohistochemistry images of human tissue samples.

    Directory of Open Access Journals (Sweden)

    Frency Varghese

    Full Text Available In anatomic pathology, immunohistochemistry (IHC serves as a diagnostic and prognostic method for identification of disease markers in tissue samples that directly influences classification and grading the disease, influencing patient management. However, till today over most of the world, pathological analysis of tissue samples remained a time-consuming and subjective procedure, wherein the intensity of antibody staining is manually judged and thus scoring decision is directly influenced by visual bias. This instigated us to design a simple method of automated digital IHC image analysis algorithm for an unbiased, quantitative assessment of antibody staining intensity in tissue sections. As a first step, we adopted the spectral deconvolution method of DAB/hematoxylin color spectra by using optimized optical density vectors of the color deconvolution plugin for proper separation of the DAB color spectra. Then the DAB stained image is displayed in a new window wherein it undergoes pixel-by-pixel analysis, and displays the full profile along with its scoring decision. Based on the mathematical formula conceptualized, the algorithm is thoroughly tested by analyzing scores assigned to thousands (n = 1703 of DAB stained IHC images including sample images taken from human protein atlas web resource. The IHC Profiler plugin developed is compatible with the open resource digital image analysis software, ImageJ, which creates a pixel-by-pixel analysis profile of a digital IHC image and further assigns a score in a four tier system. A comparison study between manual pathological analysis and IHC Profiler resolved in a match of 88.6% (P<0.0001, CI = 95%. This new tool developed for clinical histopathological sample analysis can be adopted globally for scoring most protein targets where the marker protein expression is of cytoplasmic and/or nuclear type. We foresee that this method will minimize the problem of inter-observer variations across labs and

  16. IHC Profiler: an open source plugin for the quantitative evaluation and automated scoring of immunohistochemistry images of human tissue samples.

    Science.gov (United States)

    Varghese, Frency; Bukhari, Amirali B; Malhotra, Renu; De, Abhijit

    2014-01-01

    In anatomic pathology, immunohistochemistry (IHC) serves as a diagnostic and prognostic method for identification of disease markers in tissue samples that directly influences classification and grading the disease, influencing patient management. However, till today over most of the world, pathological analysis of tissue samples remained a time-consuming and subjective procedure, wherein the intensity of antibody staining is manually judged and thus scoring decision is directly influenced by visual bias. This instigated us to design a simple method of automated digital IHC image analysis algorithm for an unbiased, quantitative assessment of antibody staining intensity in tissue sections. As a first step, we adopted the spectral deconvolution method of DAB/hematoxylin color spectra by using optimized optical density vectors of the color deconvolution plugin for proper separation of the DAB color spectra. Then the DAB stained image is displayed in a new window wherein it undergoes pixel-by-pixel analysis, and displays the full profile along with its scoring decision. Based on the mathematical formula conceptualized, the algorithm is thoroughly tested by analyzing scores assigned to thousands (n = 1703) of DAB stained IHC images including sample images taken from human protein atlas web resource. The IHC Profiler plugin developed is compatible with the open resource digital image analysis software, ImageJ, which creates a pixel-by-pixel analysis profile of a digital IHC image and further assigns a score in a four tier system. A comparison study between manual pathological analysis and IHC Profiler resolved in a match of 88.6% (P<0.0001, CI = 95%). This new tool developed for clinical histopathological sample analysis can be adopted globally for scoring most protein targets where the marker protein expression is of cytoplasmic and/or nuclear type. We foresee that this method will minimize the problem of inter-observer variations across labs and further help in

  17. Application of chemometric tools for automatic classification and profile extraction of DNA samples in forensic tasks.

    Science.gov (United States)

    Talavera Bustamante, Isneri; Silva Mata, Francisco; Hernández González, Noslen; González Gazapo, Ricardo; Palau, Juan; Ferreira, Marcia M Castro

    2007-07-09

    In this paper a method for the automatic DNA spots classification and extraction of profiles associated in DNA polyacrylamide gel electrophoresis is presented and it integrates the use of image processing techniques and chemometrics tools. A software which implements this method was developed; for feature extraction a combination of a PCA analysis and a C4.5 decision tree were used. To obtain good results in the profile extraction only DNA spots are useful; therefore, it was necessary to solve a two-class classification problem among DNA spots and no-DNA spots. In order to perform the classification process with high velocity, effectiveness and robustness, comparative classification studies among support vector machine (SVM), K-NN and PLS-DA classifiers were made. The best results obtained with the SVM classifier demonstrated the advantages attributed to it in the literature as a two-class classifier. A Sequential Cluster Leader Algorithm and another one developed for the restoration of pattern missing spots were needed to conclude the profiles extraction step. The experimental results show that this method has a very effective computational behavior and effectiveness, and provide a very useful tool to decrease the time and increase the quality of the specialist responses.

  18. Total Protein Profile and Drug Resistance in Candida albicans Isolated from Clinical Samples

    Directory of Open Access Journals (Sweden)

    Kamal Uddin Zaidi

    2016-01-01

    Full Text Available This study was done to assess the antifungal susceptibility of clinical isolates of Candida albicans and to evaluate its total protein profile based on morphological difference on drug resistance. Hundred and twenty clinical isolates of C. albicans from various clinical specimens were tested for susceptibility against four antifungal agents, namely, fluconazole, itraconazole, amphotericin B, and ketoconazole. A significant increase of drug resistance in clinical isolates of C. albicans was observed. The study showed 50% fluconazole and itraconazole resistance at 32 μg mL−1 with a MIC50 and MIC90 values at 34 and 47 and 36 and 49 μg mL−1, respectively. All isolates were sensitive to amphotericin B and ketoconazole. The SDS-PAGE protein profile showed a prevalent band of ~52.5 kDa, indicating overexpression of gene in 72% strains with fluconazole resistance. Since the opportunistic infections of Candida spp. are increasing along with drug resistance, the total protein profile will help in understanding the evolutionary changes in drug resistance and also to characterize them.

  19. Sterile paper points as a bacterial DNA-contamination source in microbiome profiles of clinical samples

    NARCIS (Netherlands)

    van der Horst, J.; Buijs, M.J.; Laine, M.L.; Wismeijer, D.; Loos, B.G.; Crielaard, W.; Zaura, E.

    2013-01-01

    Objectives High throughput sequencing of bacterial DNA from clinical samples provides untargeted, open-ended information on the entire microbial community. The downside of this approach is the vulnerability to DNA contamination from other sources than the clinical sample. Here we describe

  20. Fire Service Training. LP and Compressed Gas Emergencies.

    Science.gov (United States)

    North Carolina State Dept. of Community Colleges, Raleigh.

    Part of a series of instructional outlines intended for use in a training program for firemen with no previous formal training, this curriculum guide discusses safety precautions for liquefied petroleum (LP) and compressed gas emergencies. During its twelve hours of instruction it covers (1) terminology; (2) the chemical composition of the gases;…

  1. 2-Summing operators on C([0,1],lp)

    Indian Academy of Sciences (India)

    In this paper, in case s = 2, we give necessary and sufficient conditions that natural operators on C([0, 1],lp) with values in l1 satisfies (α), (β) and (γ), which show that the above implication is the best possible result. Keywords. Banach spaces of continuous functions; tensor products; operator ideals; p-summing operators. 1.

  2. Optimization of lipase production by Staphylococcus sp. Lp12 ...

    African Journals Online (AJOL)

    A bacterial strain isolated from an oil contaminated soil, identified as Staphylococcus sp. Lp12 was screened for lipase activity on tributyrin agar and spirit blue agar medium. Maximum lipase production was observed at 48 h of growth (3.5 Eu/ml). Peptone was found to be as an ideal nitrogen source for production at a ...

  3. Somatic Coliphage Profiles of Produce and Environmental Samples from Farms in Northern México.

    Science.gov (United States)

    Bartz, Faith E; Hodge, Domonique Watson; Heredia, Norma; de Aceituno, Anna Fabiszewski; Solís, Luisa; Jaykus, Lee-Ann; Garcia, Santos; Leon, Juan S

    2016-09-01

    Somatic coliphages were quantified in 459 produce and environmental samples from 11 farms in Northern Mexico to compare amounts of somatic coliphages among different types of fresh produce and environmental samples across the production steps on farms. Rinsates from cantaloupe melons, jalapeño peppers, tomatoes, and the hands of workers, soil, and water were collected during 2011-2012 at four successive steps on each farm, from the field before harvest through the packing facility, and assayed by FastPhage MPN Quanti-tray method. Cantaloupe farm samples contained more coliphages than jalapeño or tomato (p range food safety during fruit and vegetable production.

  4. Assessing childhood maltreatment and mental health correlates of disordered eating profiles in a nationally representative sample of English females.

    Science.gov (United States)

    Armour, Cherie; Műllerová, Jana; Fletcher, Shelley; Lagdon, Susan; Burns, Carol Rhonda; Robinson, Martin; Robinson, Jake

    2016-03-01

    Previous research suggests that childhood maltreatment is associated with the onset of eating disorders (ED). In turn, EDs are associated with alternative psychopathologies such as depression and posttraumatic stress disorder (PTSD), and with suicidality. Moreover, it has been reported that various ED profiles may exist. The aim of the current study was to examine the profiles of disordered eating and the associations of these with childhood maltreatment and with mental health psychopathology. The current study utilised a representative sample of English females (N = 4206) and assessed for the presence of disordered eating profiles using Latent Class Analysis. Multinomial logistic regression was implemented to examine the associations of childhood sexual and physical abuse with the disordered eating profiles and the associations of these with PTSD, depression and suicidality. Results supported those of previous findings in that we found five latent classes of which three were regarded as disordered eating classes. Significant relationships were found between these and measures of childhood trauma and mental health outcomes. Childhood sexual and physical abuse increased the likelihood of membership in disordered eating classes and these in turn increased the likelihood of adverse mental health and suicidal outcomes.

  5. Methoxetamine: An early report on the motivations for use, effect profile and prevalence of use in a UK clubbing sample.

    Science.gov (United States)

    Winstock, Adam R; Lawn, Will; Deluca, Paolo; Borschmann, Rohan

    2016-03-01

    To assess the prevalence of use and subjective effect profile of methoxetamine among a group of polydrug users. Cross-sectional, anonymous, online survey of UK-based polydrug users was conducted. Prevalence of lifetime, last year and last month use, sourcing of the drugs, motivations for use, and subjective effect and risk profile compared with that of ketamine were measured. There were 7700 UK-based polydrug users, of whom 326 reported recent use of methoxetamine. Of the whole sample, 4.2% reported last 12 month use of methoxetamine compared with 24.5% for ketamine. The most common route of use was intranasal and the predominate effect described as psychedelic. Of the 15.5% of last year users of ketamine reporting last year use of methoxetamine, only 18.7% reported that they thought methoxetamine was less damaging to their kidneys or bladder than ketamine. Its broad effect profile, based on participants' first experience of use, was very similar to that of ketamine. Almost one-third of users reported that they did not intend to try the drug again. Methoxetamine appears to have a broadly similar effect profile to that of ketamine. Only a minority of participants were motivated to use it because they believed it was less damaging to their kidneys or bladder than ketamine. The impact of the recent temporary banning order on availability and use of both methoxetamine and ketamine should be monitored carefully. © 2015 Australasian Professional Society on Alcohol and other Drugs.

  6. A novel Whole Air Sample Profiler (WASP for the quantification of volatile organic compounds in the boundary layer

    Directory of Open Access Journals (Sweden)

    J. E. Mak

    2013-10-01

    Full Text Available The emission and fate of reactive volatile organic compounds (VOCs is of inherent interest to those studying chemical biosphere–atmosphere interactions. In-canopy VOC observations are obtainable using tower-based samplers, but the lack of suitable sampling systems for the full boundary layer has limited the availability of data characterizing the vertical structure of such gases above the canopy height and still in the boundary layer. This is an important region where many reactive VOCs are oxidized or otherwise removed. Here we describe an airborne sampling system designed to collect a vertical profile of air into a 3/8 in. OD (outer diameter tube 150 m in length. The inlet ram air pressure is used to flow sampled air through the tube, which results in a varying flow rate based on aircraft speed and altitude. Since aircraft velocity decreases during ascent, it is necessary to account for the variable flow rate into the tube. This is accomplished using a reference gas that is pulsed into the air stream so that the precise altitude of the collected air can be reconstructed post-collection. The pulsed injections are also used to determine any significant effect from diffusion/mixing within the sampling tube, either during collection or subsequent extraction for gas analysis. This system has been successfully deployed, and we show some measured vertical profiles of isoprene and its oxidation products methacrolein and methyl vinyl ketone from a mixed canopy near Columbia, Missouri.

  7. Sorption, desorption and mineralisation of the herbicides glyphosate and MCPA in samples from two Danish soil and subsurface profiles.

    Science.gov (United States)

    Sørensen, Sebastian R; Schultz, Anne; Jacobsen, Ole S; Aamand, Jens

    2006-05-01

    The vertical distribution of the sorption, desorption and mineralisation of glyphosate and MCPA was examined in samples from two contrasting soil and subsurface profiles, obtained from a sandy agricultural site and a non-agricultural clay rich site. The highest mineralisation of [14C-methylen]glyphosate, with 9.3-14.7% degraded to 14CO2 within 3 months was found in the deepest sample from the clay site. In the deeper parts of the sandy profile high sorption and low desorption of glyphosate coincided with no or minor mineralisation indicating a limited glyphosate bioavailability. MCPA was readily mineralised except in the deepest samples from both sites. The highest MCPA mineralisation was detected just below the surface layers with 72% or 44% degraded to 14CO2 at the sandy or the clay sites, respectively. MCPA sorped to a minor extent in all samples and no indications of sorption-controlled mineralisation was revealed. None of the herbicides were mineralised under anoxic conditions.

  8. Gene expression profiling of brain samples from patients with Lewy body dementia.

    Science.gov (United States)

    Pietrzak, Maciej; Papp, Audrey; Curtis, Amanda; Handelman, Samuel K; Kataki, Maria; Scharre, Douglas W; Rempala, Grzegorz; Sadee, Wolfgang

    2016-10-28

    Dementia with Lewy Bodies (DLB) is the second most common neurodegenerative disorder in the elderly. The development and progression of DLB remain unclear. In this study we used next generation sequencing to assess RNA expression profiles and cellular processes associated with DLB in the anterior cingulate cortex, a brain region affected by DLB pathology. The expression measurements were made in autopsy brain tissues from 8 DLB subjects and 10 age-matched controls using AmpliSeq technology with ion torrent sequencing. The analysis of RNA expression profiles revealed 490 differentially expressed genes, among which 367 genes were down-regulated and 123 were up-regulated. Functional enrichment analysis of genes differentially expressed in DLB indicated downregulation of genes associated with myelination, neurogenesis, and regulation of nervous system development. miRNA binding sites enriched in these mRNAs yielded a list of candidate miRNAs participating in DLB pathophysiology. Our study provides a comprehensive picture of gene expression landscape in DLB, identifying key cellular processes associated with DLB pathology. Copyright © 2016 Elsevier Inc. All rights reserved.

  9. Mutational profile of KIT and PDGFRA genes in gastrointestinal stromal tumors in Peruvian samples

    Directory of Open Access Journals (Sweden)

    José Buleje

    2015-02-01

    Full Text Available Introduction: Gastrointestinal stromal tumors (GISTs are mesenchymal neoplasms usually caused by somatic mutations in the genes KIT (c-KIT or PDGFRA. Mutation characterization has become an important exam for GIST patients because it is useful in predicting the response to the inhibitors of receptor tyrosine kinase (RTK. Objectives: The aim of this study was to determine the frequency of KIT and PDGFRA mutations in 25 GIST samples collected over two years at two national reference hospitals in Peru. There were 21 samples collected from the Instituto Nacional de Enfermedades Neoplásicas (INEN, national cancer center and 4 samples collected from Hospital A. Loayza. Methods and materials: In this retrospective study, we performed polymerase chain reaction (PCR amplification and deoxyribonucleic acid (DNA sequencing of KIT (exons 9, 11, 13, and 17 and PDGFRA (exons 12 and 18 genes in 20 FFPE (formalin-fixed, paraffin-embedded and 5 frozen GIST samples. Results: We report 21 mutations, including deletions, duplications, and missense, no mutations in 2 samples, and 2 samples with no useful DNA for further analysis. Eighty-six percent of these mutations were located in exon 11 of KIT, and 14 % were located in exon 18 of PDGFRA. Conclusions: Our study identified mutations in 21 out of 25 GIST samples from 2 referential national hospitals in Peru, and the mutation proportion follows a global tendency observed from previous studies (i.e., the majority of samples presented KIT mutations followed by a minor percentage of PDGFRA mutations. This study presents the first mutation data of the KIT and PDGFRA genes from Peruvian individuals with GIST.

  10. Application of a new vertical profiling tool (ESASS) for sampling groundwater quality during hollow-stem auger drilling

    Science.gov (United States)

    Harte, Philip T.; Flanagan, Sarah M.

    2011-01-01

    A new tool called ESASS (Enhanced Screen Auger Sampling System) was developed by the U.S. Geological Survey. The use of ESASS, because of its unique U.S. patent design (U.S. patent no. 7,631,705 B1), allows for the collection of representative, depth-specific groundwater samples (vertical profiling) in a quick and efficient manner using a 0.305-m long screen auger during hollow-stem auger drilling. With ESASS, the water column in the flights above the screen auger is separated from the water in the screen auger by a specially designed removable plug and collar. The tool fits inside an auger of standard inner diameter (82.55 mm). The novel design of the system constituted by the plug, collar, and A-rod allows the plug to be retrieved using conventional drilling A-rods. After retrieval, standard-diameter (50.8 mm) observation wells can be installed within the hollow-stem augers. Testing of ESASS was conducted at one waste-disposal site with tetrachloroethylene (PCE) contamination and at two reference sites with no known waste-disposal history. All three sites have similar geology and are underlain by glacial, stratified-drift deposits. For the applications tested, ESASS proved to be a useful tool in vertical profiling of groundwater quality. At the waste site, PCE concentrations measured with ESASS profiling at several depths were comparable (relative percent difference nitrate and nitrite) over short (0.61 m) distances.

  11. 75 FR 38514 - Application to Export Electric Energy; Brookfield Energy Marketing LP

    Science.gov (United States)

    2010-07-02

    ... Application to Export Electric Energy; Brookfield Energy Marketing LP AGENCY: Office of Electricity Delivery and Energy Reliability, DOE. ACTION: Notice of application. SUMMARY: Brookfield Energy Marketing LP... power marketing agencies and other entities within the United States. The existing international...

  12. DMD-based software-configurable spatially-offset Raman spectroscopy for spectral depth-profiling of optically turbid samples.

    Science.gov (United States)

    Liao, Zhiyu; Sinjab, Faris; Gibson, Graham; Padgett, Miles; Notingher, Ioan

    2016-06-13

    Spectral depth-profiling of optically turbid samples is of high interest to a broad range of applications. We present a method for measuring spatially-offset Raman spectroscopy (SORS) over a range of length scales by incorporating a digital micro-mirror device (DMD) into a sample-conjugate plane in the detection optical path. The DMD can be arbitrarily programmed to collect/reject light at spatial positions in the 2D sample-conjugate plane, allowing spatially offset Raman measurements. We demonstrate several detection geometries, including annular and simultaneous multi-offset modalities, for both macro- and micro-SORS measurements, all on the same instrument. Compared to other SORS modalities, DMD-based SORS provides more flexibility with only minimal additional experimental complexity for subsurface Raman collection.

  13. Untargeted metabolomic profiling plasma samples of patients with lung cancer for searching significant metabolites by HPLC-MS method

    Science.gov (United States)

    Dementeva, N.; Ivanova, K.; Kokova, D.; Kurzina, I.; Ponomaryova, A.; Kzhyshkowska, J.

    2017-09-01

    Lung cancer is one of the most common types of cancer leading to death. Consequently, the search and the identification of the metabolites associated with the risk of developing cancer are very valuable. For the purpose, untargeted metabolic profiling of the plasma samples collected from the patients with lung cancer (n = 100) and the control group (n = 100) was conducted. After sample preparation, the plasma samples were analyzed using LC-MS method. Biostatistics methods were applied to pre-process the data for elicitation of dominating metabolites which responded to the difference between the case and the control groups. At least seven significant metabolites were evaluated and annotated. The most part of identified metabolites are connected with lipid metabolism and their combination could be useful for follow-up studies of lung cancer pathogenesis.

  14. Deregulation of LP gas industry and future problems; LP gas sangyo no kisei kanwa to kongo no kadai

    Energy Technology Data Exchange (ETDEWEB)

    Mori, T.; Okaya, Y.; Kaneko, Y. [The Institute of Energy Economics, Tokyo (Japan)

    1997-01-30

    This paper describes deregulation of LP gas industry and future problems. The conventional regulation in LP gas industry aims at the security of safety of LP gas and the proper business. The deregulation consists of the release of social regulation of safety and the reconsideration based on the introduction of competitive principle. The law was enforced from April, 1997. This law includes the proper response to advancement of safety technology, addition of self-safety incentive, open information to general consumers, and simplification of procedures. Safety and sales are separated through an establishment of safety organization. License system of sales is changed to registration system for easier participation. The current LP gas industry is loosing its competitive force due to its higher cost compared with the competitive fuels. The demand stays in a low level. There are a lot of small-scale retailers, and the enhancement of them is a problem. It is required to improve the productivity by the expansion of scale through cooperation and integration, to reduce the cost by rationalization through bulk supply and centralized monitoring, and to realize the diversification of management.

  15. Identifying student profiles and their impact on academic performance in a Brazilian undergraduate student sample.

    Science.gov (United States)

    da Silva, E T; de Fátima Nunes, M; Santos, L B; Queiroz, M G; Leles, C R

    2012-02-01

    The recognition of the student profile provides strategic information for planning educational policies in the university environment. The aims of this study were to identify natural segmentation of freshman undergraduate dental students based on demographic, socioeconomic and educational variables, and to subsequently investigate their impact on academic performance of Brazilian undergraduate students. Cluster analysis (two-step algorithm) was used to segment students who entered dental school in the time period from 1999 to 2001 (n=158) into groups based on responses to a questionnaire completed by students at the time of the admission examination. Clustering analysis revealed three natural groups. Age, the parents' level of education, and performance on the first admission test were the most important variables for cluster segmentation. Cluster 1 (n=42; 26.6%) was characterized by female students with higher socioeconomic status and better previous educational indicators. Cluster 2 (n=62; 39.2%) represented disadvantaged socioeconomic profiles, with a predominance of females and older students. Cluster 3 (n=54; 34.2%) showed similar socioeconomic characteristics to cluster 1, except for male prevalence, higher age, and experiencing difficulty in the admission test. Clusters' academic performance was satisfactory in both overall course and course groups (grade point average of at least 7.0), with average ranging from 7.89 (SD=0.44) to 8.13 (SD=0.31) and 7.37 (SD=0.75) to 8.31(SD=0.26), respectively. Our findings provide encouraging evidence for the current context of equality of access to education and reveal the importance of financial support to maximize successful educational experiences of socioeconomically disadvantaged dental students. © 2010 John Wiley & Sons A/S.

  16. VizieR Online Data Catalog: REXCESS sample optical and X-ray profiles (Holland+, 2015)

    Science.gov (United States)

    Holland, J. G.; Bohringer, H.; Chon, G.; Pierini, D.

    2015-09-01

    The REXCESS sample has been compiled as a galaxy cluster sample, representative of clusters detected by their X-ray luminosity and independent of their morphology. The sample selection is described in Bohringer et al. (2007A&A...469..363B). The X-ray observations are described in Bohringer et al. (2007A&A...469..363B). Each cluster was observed using all three detectors (MOS1, MOS2 and PN). We used the Wide Field Imager (WFI) on the Max-Planck-Gesellschaft (MPG)/European Southern Observatory (ESO) 2.2m Telescope at La Silla, which is well suited to XMM-Newton follow-up as it has a similar field of view (34-arcminx33-arcmin). Each set of optical data cover the cluster and a region outside r200 which we use for the background assessment. (3 data files).

  17. IMPACT: a whole-exome sequencing analysis pipeline for integrating molecular profiles with actionable therapeutics in clinical samples.

    Science.gov (United States)

    Hintzsche, Jennifer; Kim, Jihye; Yadav, Vinod; Amato, Carol; Robinson, Steven E; Seelenfreund, Eric; Shellman, Yiqun; Wisell, Joshua; Applegate, Allison; McCarter, Martin; Box, Neil; Tentler, John; De, Subhajyoti; Robinson, William A; Tan, Aik Choon

    2016-07-01

    Currently, there is a disconnect between finding a patient's relevant molecular profile and predicting actionable therapeutics. Here we develop and implement the Integrating Molecular Profiles with Actionable Therapeutics (IMPACT) analysis pipeline, linking variants detected from whole-exome sequencing (WES) to actionable therapeutics. The IMPACT pipeline contains 4 analytical modules: detecting somatic variants, calling copy number alterations, predicting drugs against deleterious variants, and analyzing tumor heterogeneity. We tested the IMPACT pipeline on whole-exome sequencing data in The Cancer Genome Atlas (TCGA) lung adenocarcinoma samples with known EGFR mutations. We also used IMPACT to analyze melanoma patient tumor samples before treatment, after BRAF-inhibitor treatment, and after BRAF- and MEK-inhibitor treatment. IMPACT Food and Drug Administration (FDA) correctly identified known EGFR mutations in the TCGA lung adenocarcinoma samples. IMPACT linked these EGFR mutations to the appropriate FDA-approved EGFR inhibitors. For the melanoma patient samples, we identified NRAS p.Q61K as an acquired resistance mutation to BRAF-inhibitor treatment. We also identified CDKN2A deletion as a novel acquired resistance mutation to BRAFi/MEKi inhibition. The IMPACT analysis pipeline predicts these somatic variants to actionable therapeutics. We observed the clonal dynamic in the tumor samples after various treatments. We showed that IMPACT not only helped in successful prioritization of clinically relevant variants but also linked these variations to possible targeted therapies. IMPACT provides a new bioinformatics strategy to delineate candidate somatic variants and actionable therapies. This approach can be applied to other patient tumor samples to discover effective drug targets for personalized medicine.IMPACT is publicly available at http://tanlab.ucdenver.edu/IMPACT. © The Author 2016. Published by Oxford University Press on behalf of the American Medical

  18. Profiling quinones in ambient air samples collected from the Athabasca region (Canada).

    Science.gov (United States)

    Wnorowski, Andrzej; Charland, Jean-Pierre

    2017-12-01

    This paper presents new findings on polycyclic aromatic hydrocarbon oxidation products-quinones that were collected in ambient air samples in the proximity of oil sands exploration. Quinones were characterized for their diurnal concentration variability, phase partitioning, and molecular size distribution. Gas-phase (GP) and particle-phase (PM) ambient air samples were collected separately in the summer; a lower quinone content was observed in the PM samples from continuous 24-h sampling than from combined 12-h sampling (day and night). The daytime/nocturnal samples demonstrated that nighttime conditions led to lower concentrations and some quinones not being detected. The highest quinone levels were associated with wind directions originating from oil sands exploration sites. The statistical correlation with primary pollutants directly emitted from oil sands industrial activities indicated that the bulk of the detected quinones did not originate directly from primary emission sources and that quinone formation paralleled a reduction in primary source NOx levels. This suggests a secondary chemical transformation of primary pollutants as the origin of the determined quinones. Measurements of 19 quinones included five that have not previously been reported in ambient air or in Standard Reference Material 1649a/1649b and seven that have not been previously measured in ambient air in the underivatized form. This is the first paper to report on quinone characterization in secondary organic aerosols originating from oil sands activities, to distinguish chrysenequinone and anthraquinone positional isomers in ambient air, and to report the requirement of daylight conditions for benzo[a]pyrenequinone and naphthacenequinone to be present in ambient air. Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

  19. Combined perianal-intrarectal (PI) lidocaine-prilocaine (LP) cream and lidocaine-ketorolac gel provide better pain relief than combined PI LP cream and periprostatic nerve block during transrectal prostate biopsy.

    Science.gov (United States)

    Cormio, Luigi; Pagliarulo, Vincenzo; Lorusso, Fabrizio; Selvaggio, Oscar; Perrone, Antonia; Sanguedolce, Francesca; Bufo, Pantaleo; Carrieri, Giuseppe

    2012-06-01

    What's known on the subject? and What does the study add? Study Type - Harm Reduction RCT Level of Evidence 1b The combination of perianal-intrarectal lidocaine-prilocaine cream and periprostatic nerve block effectively counteracts probe and sampling related pain during transrectal prostate biopsy, but not pain due to periprostatic infiltration. The novel combination of lidocaine-prilocaine cream and lidocaine-ketorolac gel, both administered perianal-intrarectally, provides the same probe and sampling-related pain relief than combined perianal-intrarectal lidocaine-prilocaine cream and periprostatic nerve block and prevents the non-negligible pain due to periprostatic infiltration, thus leading to better overall patients' compliance to the procedure. • To compare the efficacy and safety of combined perianal-intrarectal (PI) lidocaine-prilocaine (LP) cream and lidocaine-ketorolac (LK) gel with combined PI LP cream and periprostatic nerve block (PPNB) in relieving pain during transrectal ultrasonography guided prostate biopsy (TPB). • In all, 200 patients were randomized to receive combined PI LP cream and LK gel (group 1) or combined PI LP cream and PPNB (group 2) before TPB. • The 0-10-point visual analogue scale (VAS) was used for assessing pain at probe insertion and movements (VAS-1), periprostatic infiltration (VAS-2) when applied, and prostate sampling (VAS-3), as well as maximal procedural pain (MPP). • Complications occurring ≤ 20 days after the TPB were recorded. • The groups were comparable for patients' age, serum PSA level, prostate volume, and cancer detection rate. • All patients tolerated the procedure well. The two anaesthetic regimens provided almost equal mean VAS-1 (0.33 vs 0.37; P= 0.701) and VAS-3 (0.52 vs 0.51; P= 0.954) scores, but patients in group 2 reported significantly greater MPP scores (0.68 vs 1.53; P PI LP cream and LK gel provided the same probe- and sampling- related pain relief as combined PI LP and PPNB; moreover

  20. LP Norm SAR Tomography by Iteratively Rewighted Least Square: First Results on Hong Kong

    Science.gov (United States)

    Mancon, Simone; Tebaldini, Stefano; Monti Guarnieri, Andre

    2014-11-01

    Synthetic aperture radar tomography (TomoSAR) is the natural extension to 3-D of conventional 2-D Synthetic Aperture Radar (SAR) imaging. In this work, we focus on urban scenarios where targets of interest are point-like and radiometrically strong, i.e. the reflectivity profile in elevation is sparse. Accordingly, the method for TomoSAR imaging suggested in this work is based on Compressive Sensing (CS) theory. CS problems are typically solved by looking for the minimal solution in some Lp norm, where 0≤ p ≤ 1. The solution that minimizes an arbitrary Lp norm can be obtained using the Iteratively Reweighted Least Square (IRLS) algorithm. Based on an experimental comparison among different choices for p, the conclusion drawn is that the usual choice p = 1 is the best trade-off between resolution and robustness to noise. Results from real data will be discussed by reporting a TomoSAR reconstruction of an area in Hong Kong (China), acquired by COSMO-SkyMed.

  1. Space charge profiles in low density polyethylene samples containing a permittivity/conductivity gradient

    DEFF Research Database (Denmark)

    Bambery, K.R.; Fleming, R.J.; Holbøll, Joachim

    2001-01-01

    Laser induced pressure pulse space charge measurements were made on 1.5 mm thick plaques of high purity low density polyethylene equipped with vacuum-evaporated aluminium electrodes. Temperature differences up to 20 °C were maintained across the samples, which were subjected to dc fields up to 1...

  2. RNA sample preparation applied to gene expression profiling for the horse biological passport.

    Science.gov (United States)

    Bailly-Chouriberry, Ludovic; Baudoin, Florent; Cormant, Florence; Glavieux, Yohan; Loup, Benoit; Garcia, Patrice; Popot, Marie-Agnès; Bonnaire, Yves

    2017-09-01

    The improvement of doping control is an ongoing race. Techniques to fight doping are usually based on the direct detection of drugs or their metabolites by analytical methods such as chromatography hyphenated to mass spectrometry after ad hoc sample preparation. Nowadays, omic methods constitute an attractive development and advances have been achieved particularly by application of molecular biology tools for detection of anabolic androgenic steroids (AAS), erythropoiesis-stimulating agent (ESA), or to control human growth hormone misuses. These interesting results across different animal species have suggested that modification of gene expression offers promising new methods of improving the window of detection of banned substances by targeting their effects on blood cell gene expression. In this context, the present study describes the possibility of using a modified version of the dedicated Human IVD (in vitro Diagnostics) PAXgene® Blood RNA Kit for horse gene expression analysis in blood collected on PAXgene® tubes applied to the horse biological passport. The commercial kit was only approved for human blood samples and has required an optimization of specific technical requirements for equine blood samples. Improvements and recommendations were achieved for sample collection, storage and RNA extraction procedure. Following these developments, RNA yield and quality were demonstrated to be suitable for downstream gene expression analysis by qPCR techniques. Copyright © 2017 John Wiley & Sons, Ltd. Copyright © 2017 John Wiley & Sons, Ltd.

  3. Antibiotic resistance profile of bacteria isolated from raw milk samples of cattle and buffaloes

    Directory of Open Access Journals (Sweden)

    Tahlina Tanzin

    2016-03-01

    Conclusion: Two different species of bacteria i.e., S. aureus and E. coli are contaminating with milk samples. The pathogenic bacteria can be controlled effectively by using Ciprofloxacin and Levofloxacin in the case of mastitis in cattle and buffaloes in Bangladesh. [J Adv Vet Anim Res 2016; 3(1.000: 62-67

  4. A test for comparing two groups of samples when analyzing multiple omics profiles

    NARCIS (Netherlands)

    Chaturvedi, N.; Goeman, J.J.; Boer, J.M.A.; Wieringen, W.N. van; Menezes, R.X. de

    2014-01-01

    BACKGROUND: A number of statistical models has been proposed for studying the association between gene expression and copy number data in integrated analysis. The next step is to compare association patterns between different groups of samples. RESULTS: We propose a method, named dSIM, to find

  5. A test for comparing two groups of samples when analyzing multiple omics profiles

    NARCIS (Netherlands)

    N. Chaturvedi (Nimisha); J.J. Goeman (Jelle); J.M. Boer (Judith); W.N. Wieringen (Wessel); R.X. de Menezes (Renee)

    2014-01-01

    textabstractBackground: A number of statistical models has been proposed for studying the association between gene expression and copy number data in integrated analysis. The next step is to compare association patterns between different groups of samples.Results: We propose a method, named dSIM, to

  6. A test for comparing two groups of samples when analyzing multiple omics profiles

    NARCIS (Netherlands)

    Chaturvedi, N.; Goeman, J.J.; Boer, J.M.; van Wieringen, W.N.; Menezes, R.

    2014-01-01

    Background: A number of statistical models has been proposed for studying the association between gene expression and copy number data in integrated analysis. The next step is to compare association patterns between different groups of samples.Results: We propose a method, named dSIM, to find

  7. Mass-spectra-based peak alignment for automatic nontargeted metabolic profiling analysis for biomarker screening in plant samples.

    Science.gov (United States)

    Fu, Hai-Yan; Hu, Ou; Zhang, Yue-Ming; Zhang, Li; Song, Jing-Jing; Lu, Peang; Zheng, Qing-Xia; Liu, Ping-Ping; Chen, Qian-Si; Wang, Bing; Wang, Xiao-Yu; Han, Lu; Yu, Yong-Jie

    2017-09-01

    Nontargeted metabolic profiling analysis is a difficult task in a routine investigation because hundreds of chromatographic peaks are eluted within a short time, and the time shift problem is severe across samples. To address these problems, the present work developed an automatic nontargeted metabolic profiling analysis (anTMPA) method. First, peaks from the total ion chromatogram were extracted using modified multiscale Gaussian smoothing method. Then, a novel peak alignment strategy was employed based on the mass spectra and retention times of the peaks in which the maximum mass spectral correlation coefficient path was extracted using a modified dynamic programming method. Moreover, an automatic landmark peak-searching strategy was employed for self-adapting time shift modification. Missing peaks across samples were grouped and registered into the aligned peak list table for final refinement. Finally, the aligned peaks across samples were analyzed using statistical methods to identify potential biomarkers. Mass spectral information on the screened biomarkers could be directly imported into the National Institute of Standards and Technology library to select the candidate compounds. The performance of the anTMPA method was evaluated using a complicated plant gas chromatography-mass spectrometry dataset with the aim of identifying biomarkers between the growth and maturation stages of the tested plant. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Microbial Indicator Profiling of Fresh Produce and Environmental Samples from Farms and Packing Facilities in Northern Mexico.

    Science.gov (United States)

    Heredia, Norma; Caballero, Cindy; Cárdenas, Carmen; Molina, Karina; García, Rafael; Solís, Luisa; Burrowes, Vanessa; Bartz, Faith E; de Aceituno, Anna Fabiszewski; Jaykus, Lee-Ann; García, Santos; Leon, Juan

    2016-07-01

    To compare microbiological indicator and pathogen contamination among different types of fresh produce and environmental samples along the production chain, 636 samples of produce (rinsates from cantaloupe melons, jalapeño peppers, and tomatoes) and environmental samples (rinsates from hands of workers, soil, and water) were collected at four successive steps in the production process (from the field before harvest through the packing facility) on 11 farms in northern Mexico during 2011 and 2012. Samples were assayed for enteric pathogens (Escherichia coli O157:H7, other Shiga toxigenic E. coli, Salmonella, and Listeria monocytogenes) and microbial indicators (coliforms, other E. coli strains, and Enterococcus spp.). Salmonella was the only pathogen detected; it was found in one preharvest jalapeño sample (detection limits: 0.0033 CFU/ml in produce and hand samples, 0.0013 CFU/ml in water, and 0.04 CFU/g in soil). Microbial indicator profiles for produce, worker hands, and soil from jalapeño and tomato farms were similar, but cantaloupe farm samples had higher indicator levels (P coliforms, E. coli, and Enterococcus, respectively, and lower E. coli levels in soil (water from tomato farms, E. coli indicators were significantly more prevalent (70 to 89% of samples were positive; P = 0.01 to 0.02), and geometric mean levels were higher (0.3 to 0.6 log CFU/100 ml) than those in cantaloupe farm water (32 to 38% of samples were positive, geometric mean <1 CFU/100 ml). Microbial indicators were present during all production steps, but prevalence and levels were generally highest at the final on-farm production step (the packing facility) (P < 0.03 for significant comparisons). The finding that microbial contamination on produce farms is influenced by produce type and production step can inform the design of effective approaches to mitigate microbial contamination.

  9. Career anchor profiles of a sample of business and professional women

    Directory of Open Access Journals (Sweden)

    Ronel Erwee

    1990-06-01

    Full Text Available The career anchors of a sample of 95 business and professional woman were studied by means of the Career Orientations Inventory (DeLong, 1982. The hierarchy of career anchors of the sample was as follows: Service, Variety, Security (job tenure, Managerial competence. Autonomy, Identity, Technical/functional competence, Entrepreneurship and Security (geographical location. A few significant differences in career anchors did occur between various occupational groups. The implicatons of these findings in terms of career management in organisations are discussed. Opsomming Die loopbaanankers van 'n steekproef van 95 sake- en beroepsvroue is bestudeer deur middel van die "Career Orientations Inventory" (DeLong, 1982. Die steekproef het die volgende hierargie van loopbaanankers getoon: Diens, Verskeidenheid, Sekuriteit (organisatories, Bestuursbevoegdheid, Outonomie, Identiteit, Tegnies/funksionele bevoegdheid, Entrepreneurskap en Sekuriteit (geografies. 'n Paar beduidende verskille in loopbaanankerprofiele het tussen die onderskeie beroepsgroepe voorgekom. Die implikasies van die bevindings ten opsigte van loopbaanbeplanning in ondernemings word bespreek.

  10. Prevalence profile of odontogenic cysts and tumors on Brazilian sample after the reclassification of odontogenic keratocyst.

    Science.gov (United States)

    Jaeger, Filipe; de Noronha, Mariana Saturnino; Silva, Maiza Luiza Vieira; Amaral, Márcio Bruno Figueiredo; Grossmann, Soraya de Mattos Carmago; Horta, Martinho Campolina Rebello; de Souza, Paulo Eduardo Alencar; de Aguiar, Maria Cássia Ferreira; Mesquita, Ricardo Alves

    2017-02-01

    The aim of this study was to evaluate the impact of the reclassification of odontogenic keratocyst (OKC) as a tumor on the prevalence profile of odontogenic cysts (OCs) and odontogenic tumors (OTs). Two referral Oral and Maxillofacial Pathology services in Brazil were evaluated. All cases diagnosed as OCs or OTs were selected and classified according to the 1992 WHO-classification (cases before 2005 WHO classification of tumors excluding OKC) and the 2005 WHO classification of tumors, going forward including cases of odontogenic keratocyst tumor (KCOT). The frequency and prevalence of OCs and OTs were compared before and after the reclassification. Among 27,854 oral biopsies, 4920 (17.66%) were OCs and 992 (3.56%) were OTs. The prevalence of OTs before 2005 WHO classification of tumors was 2.04%, while the prevalence after 2005 WHO classification was 11.51% (p tumor diagnosed was odontoma with 194 cases (39.67%), and after 2005 WHO classification of tumors the KCOT was the most frequent with 207 cases (41.07%). The increase in the prevalence of OTs after 2005 WHO is related to the improvement of pathology services and to the inclusion of KCOT in the OTs group. Copyright © 2016 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

  11. Sampling Modification Effects in the Subgingival Microbiome Profile of Healthy Children

    OpenAIRE

    Santigli, Elisabeth; Trajanoski, Slave; Eberhard, Katharina; Klug, Barbara

    2017-01-01

    Background: Oral microbiota are considered major players in the development of periodontal diseases. Thorough knowledge of intact subgingival microbiomes is required to elucidate microbial shifts from health to disease. Aims: This comparative study investigated the subgingival microbiome of healthy children, possible inter- and intra-individual effects of modified sampling, and basic comparability of subgingival microprints. Methods: In five 10-year-old children, biofilm was collected from th...

  12. Direct molecular diagnosis of aspergillosis and CYP51A profiling from respiratory samples of French patients

    Directory of Open Access Journals (Sweden)

    Yanan Zhao

    2016-07-01

    Full Text Available Background: Microbiological diagnosis of aspergillosis and triazole resistance is limited by poor culture yield. To better estimate this shortcoming, we compared culture and molecular detection of A. fumigatus in respiratory samples from French patients at risk for aspergillosis. Methods: A total of 97 respiratory samples including bronchoalveolar lavages (BAL, bronchial aspirates (BA, tracheal aspirates, sputa, pleural fluids, and lung biopsy were collected from 33 patients having invasive aspergillosis (n=12, chronic pulmonary aspergillosis (n=3, allergic bronchopulmonary aspergillosis (n=7 or colonization (n=11 and 28 controls. Each specimen was evaluated by culture, pan-Aspergillus qPCR, and CYP51A PCR and sequencing. Results: One A. flavus and 19 A. fumigatus with one multiazole resistant strain (5.3% were cultured from 20 samples. Culture positivity was 62.5%, 75%, 42.9%, and 15.8% in ABPA, CPA, IA and colonized patients, respectively. Aspergillus detection rate was significantly higher by pan-Aspergillus qPCR than by culture in IA (90.5% vs 42.9%; P<0.05 and colonization group (73.7% vs 15.8%; P<0.05. The CYP51A PCR found one TR34/L98H along with 5 novel cyp51A mutations (4 non-synonymous and 1 promoter mutations, yet no association can be established currently between these novel mutations and azole resistance. The analysis of 11 matched pairs of BA and BAL samples found that 9/11 BA carried greater fungal load than BAL and CYP51A detection was more sensitive in BA than in BAL. Conclusion: Direct molecular detection of Aspergillus spp. and azole resistance markers are useful adjunct tools for comprehensive aspergillosis diagnosis. The observed superior diagnostic value of BAs to BAL fluids warrants more in-depth study.

  13. Metabolite profiling on wheat grain to enable a distinction of samples from organic and conventional farming systems.

    Science.gov (United States)

    Bonte, Anja; Neuweger, Heiko; Goesmann, Alexander; Thonar, Cécile; Mäder, Paul; Langenkämper, Georg; Niehaus, Karsten

    2014-10-01

    Identification of biomarkers capable of distinguishing organic and conventional products would be highly welcome to improve the strength of food quality assurance. Metabolite profiling was used for biomarker search in organic and conventional wheat grain (Triticum aestivum L.) of 11 different old and new bread wheat cultivars grown in the DOK system comparison trial. Metabolites were extracted using methanol and analysed by gas chromatography-mass spectrometry. Altogether 48 metabolites and 245 non-identified metabolites (TAGs) were detected in the cultivar Runal. Principal component analysis showed a sample clustering according to farming systems and significant differences in peak areas between the farming systems for 10 Runal metabolites. Results obtained from all 11 cultivars indicated a greater influence of the cultivar than the farming system on metabolite concentrations. Nevertheless, a t-test on data of all cultivars still detected 5 metabolites and 11 TAGs with significant differences between the farming systems. Based on individual cultivars, metabolite profiling showed promising results for the categorization of organic and conventional wheat. Further investigations are necessary with wheat from more growing seasons and locations before definite conclusions can be drawn concerning the feasibility to evolve a combined set of biomarkers for organically grown wheat using metabolite profiles. © 2014 Society of Chemical Industry.

  14. Neuropsychological profile of pre-schoolers with metaphonological difficulties: results from a non-clinical sample.

    Science.gov (United States)

    Termine, C; Stella, G; Capsoni, C; Rosso, E; Binda, A; Pirola, A; Conti, C; Gruppi, E; Lanzi, G; Salini, S; Tognatti, C; Zoppello, M; Balottin, U

    2007-11-01

    The level of language development reached in pre-school age is considered the most reliable predictor of reading acquisition. In normally developing children, learning to read is strongly related to early language skills, and in particular to phonological processing abilities. In dyslexic children, reading abilities seem to show a correlation with phonological awareness. A group of 65 children (aged 5-6 years) were recruited and submitted to an in-depth neuropsychological assessment [i.e. metaphonological skills, intelligence, verbal short-term memory (VSTM) and other aspects of receptive and expressive language]. We were able to identify 14 children with significant metaphonological difficulties (MD): 11 children with exclusively MD, and the other three children with specific language impairment. This study compares the neuropsychological profile obtained from children with MD with that of a peer group without any language impairment (N). The performances of the MD were within the normal ability range in almost all the administered tests but significantly lower compared with those of their peers without language impairment (N) in some items of the intelligence scale (Wechsler Preschool and Primary Scale of Intelligence) and in the tests of VSTM and of receptive/expressive language. Nevertheless, there were not statistically significant differences between MD and N in output phonology. In pre-school age, in a group of non-clinical children, with a range of abilities, those with MD appear to be at the lower end of the normal range in many other verbal skills. These children could be considered at-risk for possible subsequent difficulties learning to read and thus need to be identified and to warrant prompt treatment.

  15. Biases during DNA extraction affect bacterial and archaeal community profile of anaerobic digestion samples.

    Science.gov (United States)

    Roopnarain, Ashira; Mukhuba, Mashudu; Adeleke, Rasheed; Moeletsi, Mokhele

    2017-12-01

    The anaerobic digestion (AD) of organic waste for biogas production has received much attention in recent years due to the increasing need for renewable energy and environmentally friendly waste management systems. Identification of the microbial community involved in AD aids in better understanding and optimising of the process. The choice of DNA extraction method is an integral step in any molecular biodiversity study. In the present study, potential biases introduced by DNA extraction methods were examined by comparing quality, quantity and representability of DNA extracted from AD samples using various extraction methods. In spite of the non-kit based method (cetyltrimethylammonium bromide) yielding the largest quantity of DNA (approximately 44 µg DNA per gram dry weight), the extracted DNA contained PCR inhibitors. Furthermore, the quantity of extracted DNA was not proportional to species diversity. Diversity, determined using denaturing gradient gel electrophoresis (DGGE), was strongly linked to the type of extraction method used. The spin-column filter-based kit that incorporated mechanical and chemical lysis (Macherey-Nagel kit) gave the best results in terms of bacterial and archaeal diversity (Shannon-Wiener indices: average 2.5 and 2.6, respectively). Furthermore, this kit was the most effective at lysing hard-to-lyse bacterial and archaeal cells. The choice of DNA extraction method significantly influences the reliability and comparability of results obtained during AD microbial ecology investigations. Moreover, the careful selection of the DNA extraction method is of particular importance when analysing AD samples since these samples are rich in PCR inhibitors and hard-to-lyse cells such as archaea and gram-positive bacteria.

  16. MPLEx: a method for simultaneous pathogen inactivation and extraction of samples for multi-omics profiling

    Energy Technology Data Exchange (ETDEWEB)

    Burnum-Johnson, Kristin E.; Kyle, Jennifer E.; Eisfeld, Amie J.; Casey, Cameron P.; Stratton, Kelly G.; Gonzalez, Juan F.; Habyarimana, Fabien; Negretti, Nicholas M.; Sims, Amy C.; Chauhan, Sadhana; Thackray, Larissa B.; Halfmann, Peter J.; Walters, Kevin B.; Kim, Young-Mo; Zink, Erika M.; Nicora, Carrie D.; Weitz, Karl K.; Webb-Robertson, Bobbie-Jo M.; Nakayasu, Ernesto S.; Ahmer, Brian; Konkel, Michael E.; Motin, Vladimir; Baric, Ralph S.; Diamond, Michael S.; Kawaoka, Yoshihiro; Waters, Katrina M.; Smith, Richard D.; Metz, Thomas O.

    2017-01-01

    The continued emergence and spread of infectious agents is of increasing concern due to increased population growth and the associated increased livestock production to meet food demands, increased urbanization and land-use changes, and greater travel. A systems biology approach to infectious disease research can significantly advance our understanding of host-pathogen relationships and facilitate the development of new therapies and vaccines. Molecular characterization of infectious samples outside of appropriate biosafety containment can only take place subsequent to pathogen inactivation. Herein, we describe a modified Folch extraction using chloroform/methanol that facilitates the molecular characterization of infectious samples by enabling simultaneous pathogen inactivation and extraction of proteins, metabolites, and lipids for subsequent mass spectrometry-based multi-omics measurements. This metabolite, protein and lipid extraction (MPLEx) method resulted in complete inactivation of bacterial and viral pathogens with exposed lipid membranes, including Yersinia pestis, Salmonella Typhimurium, and Campylobacter jejuni in pure culture, and Yersinia pestis, Campylobacter jejuni, West Nile, MERS-CoV, Ebola, and influenza H7N9 viruses in infection studies. Partial inactivation was observed for pathogens without exposed lipid membranes including 99.99% inactivation of community-associated methicillin-resistant Staphylococcus aureus, 99.6% and >99% inactivation of Clostridium difficile spores and vegetative cells, respectively, and 50% inactivation of adenovirus type 5. To demonstrate that MPLEx yields biomaterial of sufficient quality for subsequent multi-omics analyses, we highlight select proteomics, metabolomics and lipidomics data from human epithelial lung cells infected with wild-type and mutant forms of influenza H7N9. We believe that MPLEx will facilitate systems biology studies of infectious samples by enabling simultaneous pathogen inactivation and multi

  17. Comparison of Dilution, Filtration, and Microwave Digestion Sample Pretreatments in Elemental Profiling of Wine by ICP-MS

    Directory of Open Access Journals (Sweden)

    Joshua Godshaw

    2017-09-01

    Full Text Available Wine elemental composition varies by cultivar, geographic origin, viticultural and enological practices, and is often used for authenticity validation. Elemental analysis of wine by Inductively Coupled Plasma Mass Spectrometry (ICP-MS is challenging due to the potential for non-spectral interferences and plasma instability arising from organic matrix components. Sample preparation mitigates these interferences, however, conflicting recommendations of best practices in ICP-MS analysis of wine have been reported. This study compared direct dilution, microwave-assisted acid digestion, and two filtration sample pretreatments, acidification prior to filtration and filtration followed by acidification, in elemental profiling of one white and three red table wines by ICP-MS. Of 43 monitored isotopes, 37 varied by sample preparation method, with significantly higher results of 17 isotopes in the microwave-digested samples. Both filtration treatments resulted in lower results for 11 isotopes compared to the other methods. Finally, isotope dilution determination of copper based on natural abundances and the 63Cu:65Cu instrument response ratio agreed with external calibration and confirmed a significant sample preparation effect. Overall, microwave digestion did not compare favorably, and direct dilution was found to provide the best compromise between ease of use and result accuracy and precision, although all preparation strategies were able to differentiate the wines.

  18. HeLP-Diabetes: randomised controlled trial protocol.

    Science.gov (United States)

    Murray, Elizabeth; Dack, Charlotte; Barnard, Maria; Farmer, Andrew; Li, Jinshuo; Michie, Susan; Pal, Kingshuk; Parrott, Steve; Ross, Jamie; Sweeting, Michael; Wood, Bindie; Yardley, Lucy

    2015-12-29

    Type 2 Diabetes Mellitus (T2DM) is common, affecting nearly 400 million people worldwide. Achieving good health for people with T2DM requires active self-management; however, uptake of self-management education is poor, and there is an urgent need to find better, more acceptable, cost-effective methods of providing self-management support. Web-based self-management support has many potential benefits for patients and health services. The aim of this trial is to determine the effectiveness and cost-effectiveness of a web-based self-management support programme for people with T2DM. This will be a multi-centre individually randomised controlled trial in primary care, recruiting adults with T2DM who are registered with participating general practices in England. Participants will be randomised to receive either an evidence-based, theoretically informed, web-based self-management programme for people with T2DM which addresses medical, emotional, and role management, called Healthy Living for People with type 2 Diabetes (HeLP-Diabetes) or a simple information website. The joint primary outcomes are glycated haemoglobin (HbA1c) and diabetes-related distress, measured by the Problem Areas In Diabetes (PAID) questionnaire. Secondary outcomes include cardiovascular risk factors, depression and anxiety, and self-efficacy for self-management of diabetes. Health economic data include health service use, costs due to the intervention, and EQ-5D for calculation of Quality Adjusted Life Years (QALYS). Data will be collected at baseline, 3 months and 12 months, with the primary endpoint at 12 months. Practice nurses, blinded to patient allocation, collect clinical data; patients complete online questionnaires for patient reported measures. A sample size of 350 recruited participants allows for attrition of up to 15 % and will provide 90 % power of detecting at a 5 % significance level a true average difference in the PAID score of 4.0 and 0.25 % change in HbA1c (both small effect

  19. Chemical characterization of a commercial Commiphora wightii resin sample and chemical profiling to assess for authenticity.

    Science.gov (United States)

    Ahmed, Rida; Ali, Zulfiqar; Wu, Yunshan; Kulkarni, Swapnil; Avery, Mitchell A; Choudhary, Muhammed Iqbal; Khan, Ikhlas A

    2011-06-01

    The gum resin of Commiphora wightii [(Hook. ex Stocks) Engl.] is an ayurvedic medicine for the treatment of arthritis, inflammation, obesity, lipid disorders, and cardiovascular diseases and is known as guggul. Morphologically, it is not easy to distinguish guggul from closely related gum resins of other plants. Reliability of the commercially available guggul is critical due to the high risk of adulteration. To check authenticity, a commercial guggul sample was investigated for its chemical markers and 17 metabolites were identified, including three new, 20(S),21-epoxy-3-oxocholest-4-ene (1), 8 β-hydroxy-3,20-dioxopregn-4,6-diene (2), and 5-(13' Z-nonadecenyl)resorcinol (17) from the ethyl acetate soluble part. During the current study, compounds 14- 17 were identified as constituents of Mangifera indica gum, as an adulterant in the commercial guggul sample. This discovery highlighted the common malpractices in the trade of medicinal raw material in the developing world. The structures of the compounds were deduced by the spectroscopic technique and chemical methods, as well as by comparison with the reported data. The structure of 20(S),21-epoxy-3-oxocholest-4-ene (1) was also unambiguously deduced by single-crystal X-ray diffraction technique. © Georg Thieme Verlag KG Stuttgart · New York.

  20. Depth profile of 236U/238U in soil samples in La Palma, Canary Islands

    Science.gov (United States)

    Srncik, M.; Steier, P.; Wallner, G.

    2011-01-01

    The vertical distribution of the 236U/238U isotopic ratio was investigated in soil samples from three different locations on La Palma (one of the seven Canary Islands, Spain). Additionally the 240Pu/239Pu atomic ratio, as it is a well establish tool for the source identification, was determined. The radiochemical procedure consisted of a U separation step by extraction chromatography using UTEVA® Resin (Eichrom Technologies, Inc.). Afterwards Pu was separated from Th and Np by anion exchange using Dowex 1x2 (Dow Chemical Co.). Furthermore a new chemical procedure with tandem columns to separate Pu and U from the matrix was tested. For the determination of the uranium and plutonium isotopes by alpha spectrometry thin sources were prepared by microprecipitation techniques. Additionally these fractions separated from the soil samples were measured by Accelerator Mass Spectrometry (AMS) to get information on the isotopic ratios 236U/238U, 240Pu/239Pu and 236U/239Pu, respectively. The 236U concentrations [atoms/g] in each surface layer (∼2 cm) were surprisingly high compared to deeper layers where values around two orders of magnitude smaller were found. Since the isotopic ratio 240Pu/239Pu indicated a global fallout signature we assume the same origin as the probable source for 236U. Our measured 236U/239Pu value of around 0.2 is within the expected range for this contamination source. PMID:21481502

  1. RNA Profiling for Biomarker Discovery: Practical Considerations for Limiting Sample Sizes

    Directory of Open Access Journals (Sweden)

    Danny J. Kelly

    2005-01-01

    Full Text Available We have compared microarray data generated on Affymetrix™ chips from standard (8 micrograms or low (100 nanograms amounts of total RNA. We evaluated the gene signals and gene fold-change estimates obtained from the two methods and validated a subset of the results by real time, polymerase chain reaction assays. The correlation of low RNA derived gene signals to gene signals obtained from standard RNA was poor for less to moderately abundant genes. Genes with high abundance showed better correlation in signals between the two methods. The signal correlation between the low RNA and standard RNA methods was improved by including a reference sample in the microarray analysis. In contrast, the fold-change estimates for genes were better correlated between the two methods regardless of the magnitude of gene signals. A reference sample based method is suggested for studies that would end up comparing gene signal data from a combination of low and standard RNA templates; no such referencing appears to be necessary when comparing fold-changes of gene expression between standard and low template reactions.

  2. Kvartsimaasälpäjauheen käyttö betonin osa-aineksena

    OpenAIRE

    Kynsilehto, Tuomas

    2017-01-01

    Opinnäytetyössä selvitettiin Keliber Oy:n toimittaman kvartsimaasälpäjauheen soveltuvuutta betonin osa-ainekseksi. Kvartsimaasälpäjauheen soveltuvuutta ei ollut aiemmin testattu. Työssä tehtiin kiviaineksen alkututkimukset ja selvitettiin, onko kvartsimaasälpäjauheen käyttö mahdollista lujuusominaisuuksiensa vuoksi, sekä mikä on kvartsimaasälpäjauheen vaikutus tuoreen betonin työstettävyysominaisuuksiin. Kvartsimaasälpäjauheen soveltuvuus testattiin luomalla yhdeksän eri normaalin, täryt...

  3. Metabolomics profiling of pre-and post-anesthesia plasma samples of colorectal patients obtained via Ficoll separation.

    Science.gov (United States)

    Ghini, Veronica; Unger, Florian T; Tenori, Leonardo; Turano, Paola; Juhl, Hartmut; David, Kerstin A

    2015-01-01

    (1)H NMR spectroscopy was used to investigate the metabolic consequences of general anesthesia in the plasma of two groups of patients with diagnosis for non-metastatic colorectal cancer and metastatic colorectal cancer with liver-metastasis, respectively. Patients were treated with etomidate or propofol, two frequently used sedation agents. Plasma samples were obtained via Ficoll separation. Here, we demonstrated that this procedure introduces a number of limitations for NMR-based metabolomics studies, due to the appearance of spurious signals. Nevertheless, the comparison of the (1)H NMR metabolomic profiles of patients treated with etomidate or propofol at equipotent dose ranges was still feasible and proved that both agents significantly decrease the plasma levels of several NMR-detectable metabolites. Consequently, samples collected during anesthesia are not suitable for metabolic profiling studies aimed at patient stratification, because interpersonal variability are reduced by the overall depression of metabolites levels. On the other hand, this study showed that plasma metabolomics could represent a valuable tool to monitor the effect of different sedation agents and/or the individual metabolic response to anesthesia, providing hints for an appropriate tuning of personalized sedation procedures. In our reference groups, the metabolomic signatures were slightly different in patients anesthetized with etomidate versus propofol. The importance of standardized collection procedures and availability of exhaustive metadata of the experimental design for the accurate evaluation of the significance of the observed changes in metabolites levels are critically discussed.

  4. Dødshjælp, drabsforbud og deontologi

    DEFF Research Database (Denmark)

    Dige, Morten

    2008-01-01

    Et velkendt argument imod aktiv dødshjælp har det deontologiske forbud mod drab eller pligten til ikke at slå ihjel som en central præmis. Sådanne deontologiske argumenter spiller en fremtrædende rolle i nylige publikationer fra Det Etiske Råd. For at gælde ukvalificeret, må disse argumenter imid...

  5. Sensory profiles of bread made from paired samples of organic and conventionally grown wheat grain.

    Science.gov (United States)

    Annett, L E; Spaner, D; Wismer, W V

    2007-05-01

    The Canadian hard red spring wheat cultivar "Park" was grown in 2005 in Edmonton, AB, Canada on both conventionally and organically managed land, situated less than 1 km apart. Grains from the paired wheat samples were compared for cereal-grain-quality attributes. For sensory analysis, organically and conventionally produced wheat grains were milled into flour and baked into 60% whole wheat bread. Color, texture, taste, and aroma attributes of bread were compared using the sensory technique of descriptive analysis. Organic grain contained more wholemeal protein than conventional grain (P grain quality for yeast-leavened bread. Mixograph analysis revealed that conventional flour produced stronger bread dough than organic flour (P 0.05), but the panel perceived the organic bread to be more "dense" in texture (P < or = 0.05) with smaller air cells in the appearance of the crumb (P < or = 0.05) than conventional bread.

  6. Relationship between clinical parameters and cytokine profiles in inflamed gingival tissue and serum samples from patients with chronic periodontitis.

    Science.gov (United States)

    Górska, Renata; Gregorek, Hanna; Kowalski, Jan; Laskus-Perendyk, Agnieszka; Syczewska, Małgorzata; Madaliński, Kazimierz

    2003-12-01

    The purpose of the present study was to assess the relation between clinical parameters and concentrations of the key (IL-1beta, TNF-alpha, IL-2, IFN-gamma, IL-4, IL-10) cytokines, important in the initiation and progression of periodontal diseases, within inflamed gingival tissues and serum samples from patients with severe chronic periodontitis. Twenty-five patients with severe chronic periodontitis, who had sites with probing depths (PD) > 5 mm, and 25 periodontally healthy persons were included in the study. Clinical examinations including PD, clinical attachment loss, plaque index, and bleeding index were performed before periodontal treatment. Gingival tissue biopsies were collected from one active site of each patient and from healthy individuals, and blood samples were withdrawn on the day of tissue biopsy. The concentrations of cytokines were determined by an enzyme-linked immunosorbent assay, and the relationship between their profiles in situ and in circulation with clinical parameters was analysed. The concentrations of IL-1beta, TNF-alpha, IL-2, IFN-gamma were, on average, significantly higher in serum samples and gingival tissue biopsies from periodontitis patients than in healthy controls. However, serum samples from both groups showed high individual variability of cytokine profiles, and no association between cytokine concentrations and clinical parameters of periodontitis was found. On the contrary, the levels of IL-4 and IL-10 in both kinds of samples obtained from patients and controls were generally low or even undetectable, and remained, on average, on the same level. However, the frequency of IL-4 (88% positive samples) and IL-10 (72%) was much higher in healthy gingival tissues. High concentrations of TNF-alpha, IFN-gamma and IL-2 and, especially, a high ratio of IL-1beta/IL-10 and TNF-alpha/IL-4 found in tissue biopsies from periodontitis patients, strongly correlated with the severity of periodontitis. These results indicate that high

  7. Smell of danger: an analysis of LP-gas odorization

    Energy Technology Data Exchange (ETDEWEB)

    Cain, W.S.; Turk, A.

    1985-03-01

    LP-gas derives warning properties from the odorants ethyl mercaptan or thiophane. Laboratory tests have implied that the average person has the ability to smell the odors before leaking LP-gas reaches one-fifth its lower limit of flammability. Generally, however, laboratory tests ignore or discard persons with a poor sense of smell, especially the elderly and persons with certain types of hyposmia. Some persons who apparently can smell the warning agents when directed may otherwise fail to notice or identify them. Elderly men seem particularly vulnerable to instances of incidental anosmia and olfactory agnosia. Psychophysical testing of the warning agents has been rather unsophisticated. There exists neither a standard protocol for testing nor adequate specification of the perceptual properties that might make one warning agent better than another. Without such developments, improvement in warning agents will fail to occur. Possible improvements include increases in concentration, the use of blends to insure more uniform delivery of agent and, to decrease the perceptual vulnerability of relatively insensitive people, use of agents with favorable psychophysical (stimulus-response) functions and use of agents with favorable adaptation characteristics. Even without a change in existing products, it seems advisable to learn more about the vulnerability of LP-gas users and to employ educational means to reduce risks.

  8. Mycotoxin profiling of 1000 beer samples with a special focus on craft beer

    Science.gov (United States)

    van Dam, Ruud; van Doorn, Ronald; Katerere, David; Berthiller, Franz; Haasnoot, Willem; Nielen, Michel W. F.

    2017-01-01

    Currently beer is booming, mainly due to the steady rise of craft breweries worldwide. Previous surveys for occurrence of mycotoxins in beer, were mainly focussed on industrial produced beer. The present survey reports the presence of mycotoxins in craft beer and how this compares to industrial produced beer. More than 1000 beers were collected from 47 countries, of which 60% were craft beers. A selection of 1000 samples were screened for the presence of aflatoxin B1, ochratoxin A (OTA), zearalenone (ZEN), fumonisins (FBs), T-2 and HT-2 toxins (T-2 and HT-2) and deoxynivalenol (DON) using a mycotoxin 6-plex immunoassay. For confirmatory analysis, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and applied. The 6-plex screening showed discrepancies with the LC-MS/MS analysis, possibly due to matrix interference and/or the presence of unknown mycotoxin metabolites. The major mycotoxins detected were DON and its plant metabolite deoxynivalenol-3-β-D-glucopyranoside (D3G). The 6-plex immunoassay reported the sum of DON and D3G (DON+D3G) contaminations ranging from 10 to 475 μg/L in 406 beers, of which 73% were craft beers. The popular craft beer style imperial stout, had the highest percentage of samples suspected positive (83%) with 29% of all imperial stout beers having DON+D3G contaminations above 100 μg/L. LC-MS/MS analysis showed that industrial pale lagers from Italy and Spain, predominantly contained FBs (3–69 μg/L). Besides FBs, African traditional beers also contained aflatoxins (0.1–1.2 μg/L). The presence of OTA, T-2, HT-2, ZEN, β-zearalenol, 3/15-acetyl-DON, nivalenol and the conjugated mycotoxin zearalenone 14-sulfate were confirmed in some beers. This study shows that in 27 craft beers, DON+D3G concentrations occurred above (or at) the Tolerable Daily Intake (TDI). Exceeding the TDI, may have a health impact. A better control of brewing malts for craft beer, should be put in place to circumvent this potential

  9. Mycotoxin profiling of 1000 beer samples with a special focus on craft beer.

    Directory of Open Access Journals (Sweden)

    Jeroen Peters

    Full Text Available Currently beer is booming, mainly due to the steady rise of craft breweries worldwide. Previous surveys for occurrence of mycotoxins in beer, were mainly focussed on industrial produced beer. The present survey reports the presence of mycotoxins in craft beer and how this compares to industrial produced beer. More than 1000 beers were collected from 47 countries, of which 60% were craft beers. A selection of 1000 samples were screened for the presence of aflatoxin B1, ochratoxin A (OTA, zearalenone (ZEN, fumonisins (FBs, T-2 and HT-2 toxins (T-2 and HT-2 and deoxynivalenol (DON using a mycotoxin 6-plex immunoassay. For confirmatory analysis, a liquid chromatography tandem mass spectrometry (LC-MS/MS method was developed and applied. The 6-plex screening showed discrepancies with the LC-MS/MS analysis, possibly due to matrix interference and/or the presence of unknown mycotoxin metabolites. The major mycotoxins detected were DON and its plant metabolite deoxynivalenol-3-β-D-glucopyranoside (D3G. The 6-plex immunoassay reported the sum of DON and D3G (DON+D3G contaminations ranging from 10 to 475 μg/L in 406 beers, of which 73% were craft beers. The popular craft beer style imperial stout, had the highest percentage of samples suspected positive (83% with 29% of all imperial stout beers having DON+D3G contaminations above 100 μg/L. LC-MS/MS analysis showed that industrial pale lagers from Italy and Spain, predominantly contained FBs (3-69 μg/L. Besides FBs, African traditional beers also contained aflatoxins (0.1-1.2 μg/L. The presence of OTA, T-2, HT-2, ZEN, β-zearalenol, 3/15-acetyl-DON, nivalenol and the conjugated mycotoxin zearalenone 14-sulfate were confirmed in some beers. This study shows that in 27 craft beers, DON+D3G concentrations occurred above (or at the Tolerable Daily Intake (TDI. Exceeding the TDI, may have a health impact. A better control of brewing malts for craft beer, should be put in place to circumvent this potential

  10. Mycotoxin profiling of 1000 beer samples with a special focus on craft beer.

    Science.gov (United States)

    Peters, Jeroen; van Dam, Ruud; van Doorn, Ronald; Katerere, David; Berthiller, Franz; Haasnoot, Willem; Nielen, Michel W F

    2017-01-01

    Currently beer is booming, mainly due to the steady rise of craft breweries worldwide. Previous surveys for occurrence of mycotoxins in beer, were mainly focussed on industrial produced beer. The present survey reports the presence of mycotoxins in craft beer and how this compares to industrial produced beer. More than 1000 beers were collected from 47 countries, of which 60% were craft beers. A selection of 1000 samples were screened for the presence of aflatoxin B1, ochratoxin A (OTA), zearalenone (ZEN), fumonisins (FBs), T-2 and HT-2 toxins (T-2 and HT-2) and deoxynivalenol (DON) using a mycotoxin 6-plex immunoassay. For confirmatory analysis, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and applied. The 6-plex screening showed discrepancies with the LC-MS/MS analysis, possibly due to matrix interference and/or the presence of unknown mycotoxin metabolites. The major mycotoxins detected were DON and its plant metabolite deoxynivalenol-3-β-D-glucopyranoside (D3G). The 6-plex immunoassay reported the sum of DON and D3G (DON+D3G) contaminations ranging from 10 to 475 μg/L in 406 beers, of which 73% were craft beers. The popular craft beer style imperial stout, had the highest percentage of samples suspected positive (83%) with 29% of all imperial stout beers having DON+D3G contaminations above 100 μg/L. LC-MS/MS analysis showed that industrial pale lagers from Italy and Spain, predominantly contained FBs (3-69 μg/L). Besides FBs, African traditional beers also contained aflatoxins (0.1-1.2 μg/L). The presence of OTA, T-2, HT-2, ZEN, β-zearalenol, 3/15-acetyl-DON, nivalenol and the conjugated mycotoxin zearalenone 14-sulfate were confirmed in some beers. This study shows that in 27 craft beers, DON+D3G concentrations occurred above (or at) the Tolerable Daily Intake (TDI). Exceeding the TDI, may have a health impact. A better control of brewing malts for craft beer, should be put in place to circumvent this potential problem.

  11. Metabolic Profiling and Classification of Propolis Samples from Southern Brazil: An NMR-Based Platform Coupled with Machine Learning.

    Science.gov (United States)

    Maraschin, Marcelo; Somensi-Zeggio, Amélia; Oliveira, Simone K; Kuhnen, Shirley; Tomazzoli, Maíra M; Raguzzoni, Josiane C; Zeri, Ana C M; Carreira, Rafael; Correia, Sara; Costa, Christopher; Rocha, Miguel

    2016-01-22

    The chemical composition of propolis is affected by environmental factors and harvest season, making it difficult to standardize its extracts for medicinal usage. By detecting a typical chemical profile associated with propolis from a specific production region or season, certain types of propolis may be used to obtain a specific pharmacological activity. In this study, propolis from three agroecological regions (plain, plateau, and highlands) from southern Brazil, collected over the four seasons of 2010, were investigated through a novel NMR-based metabolomics data analysis workflow. Chemometrics and machine learning algorithms (PLS-DA and RF), including methods to estimate variable importance in classification, were used in this study. The machine learning and feature selection methods permitted construction of models for propolis sample classification with high accuracy (>75%, reaching ∼90% in the best case), better discriminating samples regarding their collection seasons comparatively to the harvest regions. PLS-DA and RF allowed the identification of biomarkers for sample discrimination, expanding the set of discriminating features and adding relevant information for the identification of the class-determining metabolites. The NMR-based metabolomics analytical platform, coupled to bioinformatic tools, allowed characterization and classification of Brazilian propolis samples regarding the metabolite signature of important compounds, i.e., chemical fingerprint, harvest seasons, and production regions.

  12. Gene expression profiling of human breast tissue samples using SAGE-Seq.

    Science.gov (United States)

    Wu, Zhenhua Jeremy; Meyer, Clifford A; Choudhury, Sibgat; Shipitsin, Michail; Maruyama, Reo; Bessarabova, Marina; Nikolskaya, Tatiana; Sukumar, Saraswati; Schwartzman, Armin; Liu, Jun S; Polyak, Kornelia; Liu, X Shirley

    2010-12-01

    We present a powerful application of ultra high-throughput sequencing, SAGE-Seq, for the accurate quantification of normal and neoplastic mammary epithelial cell transcriptomes. We develop data analysis pipelines that allow the mapping of sense and antisense strands of mitochondrial and RefSeq genes, the normalization between libraries, and the identification of differentially expressed genes. We find that the diversity of cancer transcriptomes is significantly higher than that of normal cells. Our analysis indicates that transcript discovery plateaus at 10 million reads/sample, and suggests a minimum desired sequencing depth around five million reads. Comparison of SAGE-Seq and traditional SAGE on normal and cancerous breast tissues reveals higher sensitivity of SAGE-Seq to detect less-abundant genes, including those encoding for known breast cancer-related transcription factors and G protein-coupled receptors (GPCRs). SAGE-Seq is able to identify genes and pathways abnormally activated in breast cancer that traditional SAGE failed to call. SAGE-Seq is a powerful method for the identification of biomarkers and therapeutic targets in human disease.

  13. Fast filtration sampling protocol for mammalian suspension cells tailored for phosphometabolome profiling by capillary ion chromatography - tandem mass spectrometry.

    Science.gov (United States)

    Kvitvang, Hans F N; Bruheim, Per

    2015-08-15

    Capillary ion chromatography (capIC) is the premium separation technology for low molecular phosphometabolites and nucleotides in biological extracts. Removal of excessive amounts of salt during sample preparation stages is a prerequisite to enable high quality capIC separation in combination with reproducible and sensitive MS detection. Existing sampling protocols for mammalian cells used for GC-MS and LC-MS metabolic profiling can therefore not be directly applied to capIC separations. Here, the development of a fast filtration sampling protocol for mammalian suspension cells tailored for quantitative profiling of the phosphometabolome on capIC-MS/MS is presented. The whole procedure from sampling the culture to transfer of filter to quenching and extraction solution takes less than 10s. To prevent leakage it is critical that a low vacuum pressure is applied, and satisfactorily reproducibility was only obtained by usage of a vacuum pressure controlling device. A vacuum of 60mbar was optimal for filtration of multiple myeloma Jjn-3 cell cultures through 5μm polyvinylidene (PVDF) filters. A quick deionized water (DI-water) rinse step prior to extraction was tested, and significantly higher metabolite yields were obtained during capIC-MS/MS analyses in this extract compared to extracts prepared by saline and reduced saline (25%) washing steps only. In addition, chromatographic performance was dramatically improved. Thus, it was verified that a quick DI-water rinse is tolerated by the cells and can be included as the final stage during filtration. Over 30 metabolites were quantitated in JJN-3 cell extracts by using the optimized sampling protocol with subsequent capIC-MS/MS analysis, and up to 2 million cells can be used in a single filtration step for the chosen filter and vacuum pressure. The technical set-up is also highly advantageous for microbial metabolome filtration protocols after optimization of vacuum pressure and washing solutions, and the reduced salt

  14. Quantitative large scale gene expression profiling from human stem cell culture micro samples using multiplex pre-amplification.

    Science.gov (United States)

    Kibschull, Mark; Lye, Stephen J; Okino, Steven T; Sarras, Haya

    2016-01-01

    Transcriptional profiling is a powerful tool to study biological mechanisms during stem cell differentiation and reprogramming. Genome-wide methods like microarrays or next generation sequencing are expensive, time consuming, and require special equipment and bioinformatics expertise. Quantitative RT-PCR remains one of today's most widely accepted and used methods for analyzing gene expression in biological samples. However, limitations in the amount of starting materials often hinder the quantity and quality of information that could be obtained from a given sample. Here, we present a fast 4-step workflow allowing direct, column-free RNA isolation from limited human pluripotent stem cell (hPSC) cultures that is directly compatible with subsequent reverse transcription, target specific multiplex pre-amplification, and standard SYBR-Green quantitative PCR (qPCR) analysis. The workflow delivers excellent correlations in normalized gene-expression data obtained from different samples of hPSCs over a wide range of cell numbers (500-50,000 cells). We demonstrate accurate and unbiased target gene quantification in limiting stem cell cultures which allows for monitoring embryoid body differentiation and induced pluripotent stem cell (iPSC) reprogramming. This method highlights a rapid and cost effective screening process, allowing reduction of culture formats and increase of processing throughputs for various stem cell applications.

  15. A descriptive social and health profile of a community sample of adults and adolescents with Asperger syndrome.

    Science.gov (United States)

    Balfe, Myles; Tantam, Digby

    2010-11-12

    Little is known about the health and social profile of adolescents and adults with Asperger syndrome (AS) living in the community. We conducted a study to describe the living, employment and psycho-social situation of a community sample of forty two adults and adolescents with AS, and to describe these indivdiuals' experiences of accessing health services and taking medication. Most respondents (including those over eighteen years of age) lived at home with their parents. Most had trouble reading and responding to other people's feelings, and coping with unexpected changes. Difficulties with life skills, such as cleaning, washing and hygiene were prevalent. The majority of respondents were socially isolated and a large minority had been sexually or financially exploited. Almost all respondents had been bullied. Mental health problems such as anxiety or depression were common. 30% of respondents said that they regularly became violent and hit other people and 15% had attempted suicide. More positively, the majority of respondents felt that they could access health services if they had a health problem. The results of this study suggest a relatively poor social and health profile for many people with Asperger syndrome living in the community, with high levels of social problems and social exclusion, and difficulties managing day to day tasks such as washing and cleaning; these findings support the results of other studies that have examined psycho-social functioning in this group.

  16. A comparison of the Sensory Profile scores of children with autism and an age- and gender-matched sample.

    Science.gov (United States)

    Brockevelt, Barbara L; Nissen, Ranelle; Schweinle, William E; Kurtz, Eric; Larson, Kyle J

    2013-11-01

    The Centers for Disease Control and Prevention (CDC) reports that autism spectrum disorder (ASD) affects one in 88 children in the United States. The American Psychiatric Association's Diagnostic and Statistical Manual, Text Revision (DSM-IV-TR) defines ASD as a pervasive neurodevelopmental disorder characterized by qualitative impairment in communication and social interaction, and restricted, repetitive and stereotyped behavior patterns. The purpose of this study was to determine whether children with autism differ in their response to sensory input relative to typically developing age- and gender-matched peers. The Sensory Profile (SP) is a 125-item caregiver questionnaire designed to measure a child's ability to process sensory information and to profile the effect of sensory processing on daily life activity. The results of the SP of 21 participants with autism ages 3 to 9 years were compared with an age- and gender-matched sample of typically developing children. Significant differences were found across all four SP quadrants (Registration, Seeking, Sensitivity, and Avoiding) as well as eight of the nine SP factor scores. This study adds to the evidence indicating that children with autism process and respond to sensory input differently than typically-developing peers. The findings from this study support previous research findings that sensory processing differences exist between children with ASD and their typically-developing peers, as measured by the SP.

  17. A descriptive social and health profile of a community sample of adults and adolescents with Asperger syndrome

    Directory of Open Access Journals (Sweden)

    Tantam Digby

    2010-11-01

    Full Text Available Abstract Background Little is known about the health and social profile of adolescents and adults with Asperger syndrome (AS living in the community. We conducted a study to describe the living, employment and psycho-social situation of a community sample of forty two adults and adolescents with AS, and to describe these indivdiuals' experiences of accessing health services and taking medication. Findings Most respondents (including those over eighteen years of age lived at home with their parents. Most had trouble reading and responding to other people's feelings, and coping with unexpected changes. Difficulties with life skills, such as cleaning, washing and hygiene were prevalent. The majority of respondents were socially isolated and a large minority had been sexually or financially exploited. Almost all respondents had been bullied. Mental health problems such as anxiety or depression were common. 30% of respondents said that they regularly became violent and hit other people and 15% had attempted suicide. More positively, the majority of respondents felt that they could access health services if they had a health problem. Conclusions The results of this study suggest a relatively poor social and health profile for many people with Asperger syndrome living in the community, with high levels of social problems and social exclusion, and difficulties managing day to day tasks such as washing and cleaning; these findings support the results of other studies that have examined psycho-social functioning in this group.

  18. The reliability and factorial validity of the career decision profile for a sample of afrikaans-speaking students

    Directory of Open Access Journals (Sweden)

    G. P. De Bruin

    1997-06-01

    Full Text Available The purpose of this study was to examine the utility of the Career Decision Profile (CDP; Jones, 1989 for a sample of 133 Afrikaans-speaking students. The scores obtained on all six subscales showed acceptable reliability. The factor structure proposed by Jones for the Career Decision Needs dimension of the CDP was confirmed by means of a confirmatory factor analysis. In conclusion it appears that the CDP may be profitably used for the multidimensional assessment of career indecision with Afrikaans-speaking students. Opsomming Die doel van hierdie studie was om die bruikbaarheid van die Career Decision Profile (CDP; Jones, 1989 vir 'n steekproef van 133 Afrikaanssprekende studente te ondersoek. Al ses subskale het tellings met bevredigende betroubaarheidskoeffisiente opgelewer. Die faktorstruktuur wat deur Jones vir die Career Decision Needs-dimensie van die CDP gepostuleer is, is aan die hand van 'n bevestigende faktorontleding ondersteun. Ten slotte blyk dit dat die CDP nuttig aangewend kan word vir die multi-dimensionele taksering van loopbaanbesluiteloosheid by Afrikaanssprekende studente.

  19. A descriptive social and health profile of a community sample of adults and adolescents with Asperger syndrome

    Science.gov (United States)

    2010-01-01

    Background Little is known about the health and social profile of adolescents and adults with Asperger syndrome (AS) living in the community. We conducted a study to describe the living, employment and psycho-social situation of a community sample of forty two adults and adolescents with AS, and to describe these indivdiuals' experiences of accessing health services and taking medication. Findings Most respondents (including those over eighteen years of age) lived at home with their parents. Most had trouble reading and responding to other people's feelings, and coping with unexpected changes. Difficulties with life skills, such as cleaning, washing and hygiene were prevalent. The majority of respondents were socially isolated and a large minority had been sexually or financially exploited. Almost all respondents had been bullied. Mental health problems such as anxiety or depression were common. 30% of respondents said that they regularly became violent and hit other people and 15% had attempted suicide. More positively, the majority of respondents felt that they could access health services if they had a health problem. Conclusions The results of this study suggest a relatively poor social and health profile for many people with Asperger syndrome living in the community, with high levels of social problems and social exclusion, and difficulties managing day to day tasks such as washing and cleaning; these findings support the results of other studies that have examined psycho-social functioning in this group. PMID:21070680

  20. Protein profile study of clinical samples using laser induced fluorescence as the detection method: case of malignant and normal cervical tissues

    Science.gov (United States)

    Karemore, Gopal; Raja, Sujatha N.; Rai, Lavanya; Kartha, V. B.; Chidangil, Santhosh

    2009-02-01

    Protein profiles of tissue homogenates were recorded using HPLC separation and LIF detection method. The samples were collected from volunteers with clinically normal or cervical cancer conditions. It is shown that the protein profile can be classified as belonging to malignant or normal state by using hard and Fuzzy clustering methods. The study was performed to test the utility of the HPLC-LIF protein profiling method for classification of tissue samples as well as to establish a complementary method for histopathology for clinical diagnosis of the tissue as normal or malignant.

  1. Reference values for Lactate Pro 2™ in fetal blood sampling during labor: a cross-sectional study.

    Science.gov (United States)

    Birgisdottir, Brynhildur Tinna; Holzmann, Malin; Varli, Ingela Hulthén; Graner, Sofie; Saltvedt, Sissel; Nordström, Lennart

    2017-04-01

    Lactate Pro™ (LP1) is the only lactate meter evaluated for fetal scalp blood sampling (FBS) in intrapartum use. The reference values for this meter are: normal value 4.8 mmol/L. The production of this meter has been discontinued. An updated version, Lactate Pro 2TM (LP2), has been launched and is shown to be differently calibrated. The aims of the study were to retrieve a conversion equation to convert lactate values in FBS measured with LP2 to an estimated value if using LP1 and to define reference values for clinical management when using LP2. A cross-sectional study was conducted at a university hospital in Sweden. A total of 113 laboring women with fetal heart rate abnormalities on cardiotocography (CTG) had FBS carried out. Lactate concentration was measured bedside with both LP1 and LP2 from the same blood sample capillary. A linear regression model was constructed to retrieve a conversion equation to convert LP2 values to LP1 values. LP2 measured higher values than LP1 in all analyses. We found that 4.2 mmol/L with LP1 corresponded to 6.4 mmol/L with LP2. Likewise, 4.8 mmol/L with LP1 corresponded to 7.3 mmol/L with LP2. The correlation between the analyses was excellent (Spearman's rank correlation, r=0.97). We recommend the following guidelines when interpreting lactate concentration in FBS with LP2: 7.3 mmol/L as acidemia indicating intervention.

  2. A Self-Powered Fast-Sampling Profiling Float in support of a Mesoscale Ocean Observing System in the Western North Pacific

    Science.gov (United States)

    Valdez, T.; Chao, Y.; Davis, R. E.; Jones, J.

    2012-12-01

    This talk will describe a new self-powered profiling float that can perform fast sampling over the upper ocean for long durations in support of a mesoscale ocean observing system in the Western North Pacific. The current state-of-the-art profiling floats can provide several hundreds profiles for the upper ocean every ten days. To quantify the role of the upper ocean in modulating the development of Typhoons requires at least an order of magnitude reduction for the sampling interval. With today's profiling float and battery technology, a fast sampling of one day or even a few hours will reduce the typical lifetime of profiling floats from years to months. Interactions between the ocean and typhoons often involves mesoscale eddies and fronts, which require a dense array of floats to reveal the 3-dimensional structure. To measure the mesoscale ocean over a large area like the Western North Pacific therefore requires a new technology that enables fast sampling and long duration at the same time. Harvesting the ocean renewable energy associated with the vertical temperature differentials has the potential to power profiling floats with fast sampling over long durations. Results from the development and deployment of a prototype self-powered profiling float (known as SOLO-TREC) will be presented. With eight hours sampling in the upper 500 meters, the upper ocean temperature and salinity reveal pronounced high frequency variations. Plans to use the SOLO-TREC technology in support of a dense array of fast sampling profiling floats in the Western North Pacific will be discussed.

  3. A linear programming approach for estimating the structure of a sparse linear genetic network from transcript profiling data

    Directory of Open Access Journals (Sweden)

    Chandra Nagasuma R

    2009-02-01

    Full Text Available Abstract Background A genetic network can be represented as a directed graph in which a node corresponds to a gene and a directed edge specifies the direction of influence of one gene on another. The reconstruction of such networks from transcript profiling data remains an important yet challenging endeavor. A transcript profile specifies the abundances of many genes in a biological sample of interest. Prevailing strategies for learning the structure of a genetic network from high-dimensional transcript profiling data assume sparsity and linearity. Many methods consider relatively small directed graphs, inferring graphs with up to a few hundred nodes. This work examines large undirected graphs representations of genetic networks, graphs with many thousands of nodes where an undirected edge between two nodes does not indicate the direction of influence, and the problem of estimating the structure of such a sparse linear genetic network (SLGN from transcript profiling data. Results The structure learning task is cast as a sparse linear regression problem which is then posed as a LASSO (l1-constrained fitting problem and solved finally by formulating a Linear Program (LP. A bound on the Generalization Error of this approach is given in terms of the Leave-One-Out Error. The accuracy and utility of LP-SLGNs is assessed quantitatively and qualitatively using simulated and real data. The Dialogue for Reverse Engineering Assessments and Methods (DREAM initiative provides gold standard data sets and evaluation metrics that enable and facilitate the comparison of algorithms for deducing the structure of networks. The structures of LP-SLGNs estimated from the INSILICO1, INSILICO2 and INSILICO3 simulated DREAM2 data sets are comparable to those proposed by the first and/or second ranked teams in the DREAM2 competition. The structures of LP-SLGNs estimated from two published Saccharomyces cerevisae cell cycle transcript profiling data sets capture known

  4. Identification of sixteen peptides reflecting heat and/or storage induced processes by profiling of commercial milk samples.

    Science.gov (United States)

    Ebner, Jennifer; Baum, Florian; Pischetsrieder, Monika

    2016-09-16

    Peptide profiles of different drinking milk samples were examined to study how the peptide fingerprint of milk reflects processing conditions. The combination of a simple and fast method for peptide extraction using stage tips and MALDI-TOF-MS enabled the fast and easy generation and relative quantification of peptide fingerprints for high-temperature short-time (HTST), extended shelf life (ESL) and ultra-high temperature (UHT) milk of the same dairies. The relative quantity of 16 peptides changed as a function of increasing heat load. Additional heating experiments showed that among those, the intensity of peptide β-casein 196-209 (m/z 1460.9Da) was most heavily influenced by heat treatment indicating a putative marker peptide for milk processing conditions. Storage experiments with HTST- and UHT milk revealed that the differences between different types of milk samples were not only caused by the heating process. Relevant was also the proteolytic activity of enzymes during storage, which were differently influenced by the heat treatment. These results indicate that the peptide profile may be suitable to monitor processing as well as storage conditions of milk. In the present study, peptide profiling of different types of milk was carried out by MALDI-TOF-MS after stage-tip extraction and relative quantification using an internal reference peptide. Although MALDI-TOF-MS covers only part of the peptidome, the method is easy and quick and is, therefore, suited for routine analysis to address several aspects of food authenticity. Using this method, 16 native peptides were detected in milk that could be modulated by different industrial processes. Subsequent heating and storage experiments with pasteurized and UHT milk confirmed that these peptides are indeed related to the production or storage conditions of the respective products. Furthermore, the heating experiments revealed one peptide, namely the β-casein-derived sequence β-casein 196-209, which underwent

  5. Effects of apple consumption on lipid profile of hyperlipidemic and overweight men

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Vafa

    2011-01-01

    Full Text Available Objectives: Fruits and vegetables may be beneficial on lipid profile of hyperlipidemic subjects. The present study was aimed to verify the effect of golden delicious apple on Lipid Profile in hyperlipidemic and overweight men. Methods: Forty six hyperlipidemic and overweight men were randomly divided into two groups. Intervention group received 300g golden delicious apple per day for 8 weeks. Control group had the regular dietary regimen for the same period of time. Blood samples were analyzed for serum triglycerides (TG, total cholesterol (TC, low density lipoprotein-cholesterol (LDL-C, high density lipoprotein-cholesterol (HDL-C, very low density lipoprotein-cholesterol (VLDL, apolipoprotein B (Apo B, lipoprotein a (Lp a and LDL/HDL ratio at baseline and after intervention. Results: Total polyphenols and fibers were 485 mg/kg and 4.03 g/100g in fresh apple respectively. After 8 weeks, significant statistical differences were observed considering the TG and VLDL levels between two groups, but no significant differences were observed regarding TC, LDL-C, HDL-C, Apo (B, Lp (a and LDL/HDL ratio. Conclusions: Consumption of Golden delicious apple may be increased serum TG and VLDL in hyperlipidemic and overweight men. We need more studies to assay the effect of apple consumption on serum TC, LDL-C, HDL-C, Apo (B, Lp (a and LDL/HDL ratio.

  6. The profile of Porphyromonas gingivalis kgp biotype and fimA genotype mosaic in subgingival plaque samples.

    Science.gov (United States)

    Nadkarni, Mangala A; Chhour, Kim-Ly; Chapple, Cheryl C; Nguyen, Ky-Anh; Hunter, Neil

    2014-12-01

    Combined analysis of allelic variation of the virulence-associated, strain-specific lys-gingipain gene (kgp) and major fimbrial gene (fimA) of Porphyromonas gingivalis was undertaken in 116 subgingival plaque samples to understand the kgp biotype and fimA genotype profile in a subject-specific manner. Allelic variation in the polyadhesin domain of kgp from P. gingivalis strains 381 (ATCC 33277), HG66 and W83 generated four isoforms corresponding to four biotypes of P. gingivalis. Similarly, variation in the fimA subunit of the fimA gene cluster of P. gingivalis resulted in six fimA genotypes. Strain-specific differential PCR was performed for kgp and fimA using DNA isolated from subgingival plaque samples. Our findings demonstrate that all of the P. gingivalis kgp biotypes detected in this study were predominantly associated with the fimA II genotype. Dominance of kgp biotypes 381 or HG66 combined with fimA II fimbriae could imply an adaptive strategy by P. gingivalis to generate the fittest strains for survival in the host environment. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

  7. Diversity in the stellar velocity dispersion profiles of a large sample of Brightest Cluster Galaxies z ≤ 0.3

    Science.gov (United States)

    Loubser, S. I.; Hoekstra, H.; Babul, A.; O'Sullivan, E.

    2018-02-01

    We analyse spatially-resolved deep optical spectroscopy of Brightest Cluster Galaxies (BCGs) located in 32 massive clusters with redshifts of 0.05 ≤z ≤ 0.30, to investigate their velocity dispersion profiles. We compare these measurements to those of other massive early-type galaxies, as well as central group galaxies, where relevant. This unique, large sample extends to the most extreme of massive galaxies, spanning MK between -25.7 to -27.8 mag, and host cluster halo mass M500 up to 1.7 × 1015 M⊙. To compare the kinematic properties between brightest group and cluster members, we analyse similar spatially-resolved long-slit spectroscopy for 23 nearby Brightest Group Galaxies (BGGs) from the Complete Local-Volume Groups Sample (CLoGS). We find a surprisingly large variety in velocity dispersion slopes for BCGs, with a significantly larger fraction of positive slopes, unique compared to other (non-central) early-type galaxies as well as the majority of the brightest members of the groups. We find that the velocity dispersion slopes of the BCGs and BGGs correlate with the luminosity of the galaxies, and we quantify this correlation. It is not clear whether the full diversity in velocity dispersion slopes that we see is reproduced in simulations.

  8. Sparse Adaptive Channel Estimation Based on lp-Norm-Penalized Affine Projection Algorithm

    Directory of Open Access Journals (Sweden)

    Yingsong Li

    2014-01-01

    Full Text Available We propose an lp-norm-penalized affine projection algorithm (LP-APA for broadband multipath adaptive channel estimations. The proposed LP-APA is realized by incorporating an lp-norm into the cost function of the conventional affine projection algorithm (APA to exploit the sparsity property of the broadband wireless multipath channel, by which the convergence speed and steady-state performance of the APA are significantly improved. The implementation of the LP-APA is equivalent to adding a zero attractor to its iterations. The simulation results, which are obtained from a sparse channel estimation, demonstrate that the proposed LP-APA can efficiently improve channel estimation performance in terms of both the convergence speed and steady-state performance when the channel is exactly sparse.

  9. Human glioblastoma-associated microglia/monocytes express a distinct RNA profile compared to human control and murine samples.

    Science.gov (United States)

    Szulzewsky, Frank; Arora, Sonali; de Witte, Lot; Ulas, Thomas; Markovic, Darko; Schultze, Joachim L; Holland, Eric C; Synowitz, Michael; Wolf, Susanne A; Kettenmann, Helmut

    2016-08-01

    Glioblastoma (GBM) is the most aggressive brain tumor in adults. It is strongly infiltrated by microglia and peripheral monocytes that support tumor growth. In the present study we used RNA sequencing to compare the expression profile of CD11b(+) human glioblastoma-associated microglia/monocytes (hGAMs) to CD11b(+) microglia isolated from non-tumor samples. Hierarchical clustering and principal component analysis showed a clear separation of the two sample groups and we identified 334 significantly regulated genes in hGAMs. In comparison to human control microglia hGAMs upregulated genes associated with mitotic cell cycle, cell migration, cell adhesion, and extracellular matrix organization. We validated the expression of several genes associated with extracellular matrix organization in samples of human control microglia, hGAMs, and the hGAMs-depleted fraction via qPCR. The comparison to murine GAMs (mGAMs) showed that both cell populations share a significant fraction of upregulated transcripts compared with their respective controls. These genes were mostly related to mitotic cell cycle. However, in contrast to murine cells, human GAMs did not upregulate genes associated to immune activation. Comparison of human and murine GAMs expression data to several data sets of in vitro-activated human macrophages and murine microglia showed that, in contrast to mGAMs, hGAMs share a smaller overlap to these data sets in general and in particular to cells activated by proinflammatory stimulation with LPS + INFγ or TNFα. Our findings provide new insights into the biology of human glioblastoma-associated microglia/monocytes and give detailed information about the validity of murine experimental models. GLIA 2016 GLIA 2016;64:1416-1436. © 2016 Wiley Periodicals, Inc.

  10. Gemini NIFS survey of feeding and feedback processes in nearby active galaxies - II. The sample and surface mass density profiles

    Science.gov (United States)

    Riffel, R. A.; Storchi-Bergmann, T.; Riffel, R.; Davies, R.; Bianchin, M.; Diniz, M. R.; Schönell, A. J.; Burtscher, L.; Crenshaw, M.; Fischer, T. C.; Dahmer-Hahn, L. G.; Dametto, N. Z.; Rosario, D.

    2018-02-01

    We present and characterize a sample of 20 nearby Seyfert galaxies selected for having BAT 14-195 keV luminosities LX ≥ 1041.5 erg s-1, redshift z ≤ 0.015, being accessible for observations with the Gemini Near-Infrared Field Spectrograph (NIFS) and showing extended [O III]λ5007 emission. Our goal is to study Active Galactic Nucleus (AGN) feeding and feedback processes from near-infrared integral-field spectra, which include both ionized (H II) and hot molecular (H2) emission. This sample is complemented by other nine Seyfert galaxies previously observed with NIFS. We show that the host galaxy properties (absolute magnitudes MB, MH, central stellar velocity dispersion and axial ratio) show a similar distribution to those of the 69 BAT AGN. For the 20 galaxies already observed, we present surface mass density (Σ) profiles for H II and H2 in their inner ˜500 pc, showing that H II emission presents a steeper radial gradient than H2. This can be attributed to the different excitation mechanisms: ionization by AGN radiation for H II and heating by X-rays for H2. The mean surface mass densities are in the range (0.2 ≤ ΣH II ≤ 35.9) M⊙ pc-2, and (0.2 ≤ ΣH2 ≤ 13.9)× 10-3 M⊙ pc-2, while the ratios between the H II and H2 masses range between ˜200 and 8000. The sample presented here will be used in future papers to map AGN gas excitation and kinematics, providing a census of the mass inflow and outflow rates and power as well as their relation with the AGN luminosity.

  11. Ældre indvandrere med behov for (hjemme)hjælp

    DEFF Research Database (Denmark)

    Liversage, Anika

    2017-01-01

    utilstrækkelig kvalitet. Muligheden for at fravælge kommunal hjemmehjælp kan betinges af, af de ældre indvandrere i stedet modtager hjælp fra yngre, primært kvindelige, familiemedlemmer. Denne familiære hjælp kan dog betyde, at fx nogle svigerdøtre kommer til at varetage ganske krævende omsorgsopgaver. I nogle...

  12. Extremum of geometric functionals involving general L p $L_{p}$ -projection bodies

    Directory of Open Access Journals (Sweden)

    Weidong Wang

    2016-05-01

    Full Text Available Abstract Following the discovery of general L p $L_{p}$ -projection bodies by Ludwig, Haberl and Schuster determined the extremum of the volume of the polars of this family of L p $L_{p}$ -projection bodies. In this paper, the result of Haberl and Schuster is extended to all dual quermassintegrals, and a dual counterpart for the quermassintegrals of general L p $L_{p}$ -projection bodies is also obtained. Moreover, the extremum of the L q $L_{q}$ -dual affine surface areas of polars of general L p $L_{p}$ -projection bodies are determined.

  13. Neuropsychological profiles correlated with clinical and behavioral impairments in a sample of Brazilian children with Attention Deficit Hyperactivity Disorder (ADHD

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    Sueli eRizzutti

    2015-11-01

    Full Text Available ADHD is a complex neurodevelopmental disorder that implies several-step process and there is no single test to diagnose both ADHD and associated comorbidities such as oppositional defiant disorder, anxiety disorder, depression and certain types of learning disabilities. The purpose of the present study was to examine correlations between behavioral and clinical symptoms by administering an extensive neuropsychological battery to a sample of children and adolescents from a developing country. The sample was divided into three groups: non-ADHD; ADHD-non-comorbid; and ADHD+comorbidity. A full neuropsychological battery and clinical assessment found that 105 children met DSM-5 criteria, of whom 46.6% had the predominantly inattentive presentation, 37.3% had combined presentation and 16% were predominantly hyperactive/impulsive presentation. The internal correlation between neuropsychological tests did not reach statistical significance in the comparison between ADHD and non-ADHD cases (p<0.17. Clinical ADHD cases, including both +comorbidity and non-comorbid groups, performed substantially worse on CPT, working memory. Comparing ADHD-non-comorbid and ADHD+comorbidity groups, the latter did significantly worse on inhibitory control, time processing and the level of perseveration response on CPT indexes, as well as on working memory performance and CBCL tests particularly the CBCL-DESR (deficient emotional self-regulation test in the ADHD+comorbidity group. Children diagnosed as oppositional-defiant (ODD or with conduct disorder (CD showed close correlations between clinical CBCL profiles and externalized symptoms. Our findings suggest that ADHD+comorbidity and ADHD non-comorbid cases may be differentiated by a number of neuropsychological measures, such as processing speed, inhibitory control and working memory, that may reflect different levels of involvement of the hot and cool executive domains, which are more impaired in cases of severe

  14. Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

    Science.gov (United States)

    Lu, Chen; Wonsidler, Joshua L.; Li, Jianwei; Du, Yanming; Block, Timothy; Haab, Brian; Chen, Songming

    2012-01-01

    In this study, we describe an effective protocol for use in a multiplexed high-throughput antibody microarray with glycan binding protein detection that allows for the glycosylation profiling of specific proteins. Glycosylation of proteins is the most prevalent post-translational modification found on proteins, and leads diversified modifications of the physical, chemical, and biological properties of proteins. Because the glycosylation machinery is particularly susceptible to disease progression and malignant transformation, aberrant glycosylation has been recognized as early detection biomarkers for cancer and other diseases. However, current methods to study protein glycosylation typically are too complicated or expensive for use in most normal laboratory or clinical settings and a more practical method to study protein glycosylation is needed. The new protocol described in this study makes use of a chemically blocked antibody microarray with glycan-binding protein (GBP) detection and significantly reduces the time, cost, and lab equipment requirements needed to study protein glycosylation. In this method, multiple immobilized glycoprotein-specific antibodies are printed directly onto the microarray slides and the N-glycans on the antibodies are blocked. The blocked, immobilized glycoprotein-specific antibodies are able to capture and isolate glycoproteins from a complex sample that is applied directly onto the microarray slides. Glycan detection then can be performed by the application of biotinylated lectins and other GBPs to the microarray slide, while binding levels can be determined using Dylight 549-Streptavidin. Through the use of an antibody panel and probing with multiple biotinylated lectins, this method allows for an effective glycosylation profile of the different proteins found in a given human or animal sample to be developed. Introduction Glycosylation of protein, which is the most ubiquitous post-translational modification on proteins, modifies

  15. Stratigraphy of a proposed wind farm site southeast of Block Island: Utilization of borehole samples, downhole logging, and seismic profiles

    Science.gov (United States)

    Sheldon, Dane P. H.

    Seismic stratigraphy, sedimentology, lithostratigraphy, downhole geophysical logging, mineralogy, and palynology were used to study and interpret the upper 70 meters of the inner continental shelf sediments within a proposed wind farm site located approximately two to three nautical miles to the southeast of Block Island, Rhode Island. Core samples and downhole logging collected from borings drilled for geotechnical purposes at proposed wind turbine sites along with seismic surveys in the surrounding area provide the data for this study. Cretaceous coastal plain sediments that consist of non-marine to marine sand, silt, and clay are found overlying bedrock at a contact depth beyond the sampling depth of this study. The upper Cretaceous sediments sampled in borings are correlated with the Magothy/Matawan formations described regionally from New Jersey to Nantucket. An unconformity formed through sub-aerial, fluvial, marine, and glacial erosion marks the upper strata of the Cretaceous sediments separating them from the overlying deposits. The majority of Quaternary deposits overlying the unconformity represent the advance, pulsing, and retreat of the Laurentide ice sheet that reached its southern terminus in the area of Block Island approximately 25,000 to 21,000 years before present. The sequence consists of a basal glacial till overlain by sediments deposited by meltwater environments ranging from deltaic to proglacial lakefloor. A late Pleistocene to early Holocene unconformity marks the top of the glacial sequence and was formed after glacial retreat through fluvial and subaerial erosion/deposition. Overlying the glacial sequence are sediments deposited during the late Pleistocene and Holocene consisting of interbedded gravel, sand, silt, and clay. Sampling of these sediments was limited and surficial reflectors in seismic profiles were masked due to a hard bottom return. However, two depositional periods are interpreted as representing fluvial and estuarine

  16. Body Composition, Nutritional Profile and Muscular Fitness Affect Bone Health in a Sample of Schoolchildren from Colombia: The Fuprecol Study

    Science.gov (United States)

    Forero-Bogotá, Mónica Adriana; Ojeda-Pardo, Mónica Liliana; García-Hermoso, Antonio; Correa-Bautista, Jorge Enrique; González-Jiménez, Emilio; Schmidt-RíoValle, Jacqueline; Navarro-Pérez, Carmen Flores; Gracia-Marco, Luis; Vlachopoulos, Dimitris; Martínez-Torres, Javier; Ramírez-Vélez, Robinson

    2017-01-01

    The objective of the present study is to investigate the relationships between body composition, nutritional profile, muscular fitness (MF) and bone health in a sample of children and adolescents from Colombia. Participants included 1118 children and adolescents (54.6% girls). Calcaneal broadband ultrasound attenuation (c-BUA) was obtained as a marker of bone health. Body composition (fat mass and lean mass) was assessed using bioelectrical impedance analysis. Furthermore height, weight, waist circumference and Tanner stage were measured and body mass index (BMI) was calculated. Standing long-jump (SLJ) and isometric handgrip dynamometry were used respectively as indicators of lower and upper body muscular fitness. A muscular index score was also computed by summing up the standardised values of both SLJ and handgrip strength. Dietary intake and degree of adherence to the Mediterranean diet were assessed by a 7-day recall questionnaire for food frequency and the Kidmed questionnaire. Poor bone health was considered using a z-score cut off of ≤−1.5 standard deviation. Once the results were adjusted for age and Tanner stage, the predisposing factors of having a c-BUA z-score ≤−1.5 standard deviation included being underweight or obese, having an unhealthy lean mass, having an unhealthy fat mass, SLJ performance, handgrip performance, and unhealthy muscular index score. In conclusion, body composition (fat mass and lean body mass) and MF both influenced bone health in a sample of children and adolescents from Colombia. Thus promoting strength adaptation and preservation in Colombian youth will help to improve bone health, an important protective factor against osteoporosis in later life. PMID:28165360

  17. Body Composition, Nutritional Profile and Muscular Fitness Affect Bone Health in a Sample of Schoolchildren from Colombia: The Fuprecol Study

    Directory of Open Access Journals (Sweden)

    Mónica Adriana Forero-Bogotá

    2017-02-01

    Full Text Available The objective of the present study is to investigate the relationships between body composition, nutritional profile, muscular fitness (MF and bone health in a sample of children and adolescents from Colombia. Participants included 1118 children and adolescents (54.6% girls. Calcaneal broadband ultrasound attenuation (c-BUA was obtained as a marker of bone health. Body composition (fat mass and lean mass was assessed using bioelectrical impedance analysis. Furthermore height, weight, waist circumference and Tanner stage were measured and body mass index (BMI was calculated. Standing long-jump (SLJ and isometric handgrip dynamometry were used respectively as indicators of lower and upper body muscular fitness. A muscular index score was also computed by summing up the standardised values of both SLJ and handgrip strength. Dietary intake and degree of adherence to the Mediterranean diet were assessed by a 7-day recall questionnaire for food frequency and the Kidmed questionnaire. Poor bone health was considered using a z-score cut off of ≤−1.5 standard deviation. Once the results were adjusted for age and Tanner stage, the predisposing factors of having a c-BUA z-score ≤−1.5 standard deviation included being underweight or obese, having an unhealthy lean mass, having an unhealthy fat mass, SLJ performance, handgrip performance, and unhealthy muscular index score. In conclusion, body composition (fat mass and lean body mass and MF both influenced bone health in a sample of children and adolescents from Colombia. Thus promoting strength adaptation and preservation in Colombian youth will help to improve bone health, an important protective factor against osteoporosis in later life.

  18. Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry

    Directory of Open Access Journals (Sweden)

    Müller Maren

    2011-11-01

    Full Text Available Abstract Background Plant hormones play a pivotal role in several physiological processes during a plant's life cycle, from germination to senescence, and the determination of endogenous concentrations of hormones is essential to elucidate the role of a particular hormone in any physiological process. Availability of a sensitive and rapid method to quantify multiple classes of hormones simultaneously will greatly facilitate the investigation of signaling networks in controlling specific developmental pathways and physiological responses. Due to the presence of hormones at very low concentrations in plant tissues (10-9 M to 10-6 M and their different chemistries, the development of a high-throughput and comprehensive method for the determination of hormones is challenging. Results The present work reports a rapid, specific and sensitive method using ultrahigh-performance liquid chromatography coupled to electrospray ionization tandem spectrometry (UPLC/ESI-MS/MS to analyze quantitatively the major hormones found in plant tissues within six minutes, including auxins, cytokinins, gibberellins, abscisic acid, 1-amino-cyclopropane-1-carboxyic acid (the ethylene precursor, jasmonic acid and salicylic acid. Sample preparation, extraction procedures and UPLC-MS/MS conditions were optimized for the determination of all plant hormones and are summarized in a schematic extraction diagram for the analysis of small amounts of plant material without time-consuming additional steps such as purification, sample drying or re-suspension. Conclusions This new method is applicable to the analysis of dynamic changes in endogenous concentrations of hormones to study plant developmental processes or plant responses to biotic and abiotic stresses in complex tissues. An example is shown in which a hormone profiling is obtained from leaves of plants exposed to salt stress in the aromatic plant, Rosmarinus officinalis.

  19. Bile acid profiling in human biological samples: comparison of extraction procedures and application to normal and cholestatic patients.

    Science.gov (United States)

    Humbert, Lydie; Maubert, Marie Anne; Wolf, Claude; Duboc, Henri; Mahé, Myriam; Farabos, Dominique; Seksik, Philippe; Mallet, Jean Maurice; Trugnan, Germain; Masliah, Joëlle; Rainteau, Dominique

    2012-06-15

    The role of bile acids in cell metabolism, membrane biology and cell signaling is increasingly recognized, thus making necessary a robust and versatile technique to extract, separate and quantify a large concentration range of these numerous molecular species. HPLC-MS/MS analysis provides the highest sensitivity to detect and identify bile acids. However, due to their large chemical diversity, extraction methods are critical and quite difficult to optimize, as shown by a survey of the literature. This paper compares the performances of four bile acid extraction protocols applied to either liquid (serum, urine, bile) or solid (stool) samples. Acetonitrile was found to be the best solvent for deproteinizing liquid samples and NaOH the best one for stool extraction. These optimized extraction procedures allowed us to quantitate as much as 27 distinct bile acids including sulfated species in a unique 30 min HPLC run, including both hydrophilic and hydrophobic species with a high efficiency. Tandem MS provided a non ambiguous identification of each metabolite with a good sensitivity (LOQ below 20 nmol/l except for THDCA and TLCA). After validation, these methods, successfully applied to a group of 39 control patients, detected 14 different species in serum in the range of 30-800 nmol/l, 11 species in urine in the range of 20-200 nmol/l and 25 species in stool in the range of 0.4-2000 nmol/g. The clinical interest of this method has been then validated on cholestatic patients. The proposed protocols seem suitable for profiling bile acids in routine analysis. Copyright © 2012 Elsevier B.V. All rights reserved.

  20. Detection of metabolites discriminating subtypes of thyroid cancer: Molecular profiling of FFPE samples using the GC/MS approach.

    Science.gov (United States)

    Wojakowska, Anna; Chekan, Mykola; Marczak, Łukasz; Polanski, Krzysztof; Lange, Dariusz; Pietrowska, Monika; Widlak, Piotr

    2015-12-05

    One of the critical issues in thyroid cancer diagnostic is differentiation between follicular adenoma, follicular carcinoma and the follicular variant of papillary carcinoma, which in some cases is not possible based on histopathological features only. In this paper we performed molecular profiling of thyroid tissue aiming to identify metabolites characteristic for different types of thyroid cancer. FFPE tissue specimens were analysed from 5 different types of thyroid malignancies (follicular, papillary/classical variant, papillary/follicular variant, medullary and anaplastic cancers), benign follicular adenoma and normal thyroid. Extracted metabolites were identified and semi-quantified using the GC/MS approach. There were 28 metabolites identified, whose abundances were significantly different among different types of thyroid tumours, including lipids, carboxylic acids, and saccharides. We concluded, that multi-component metabolome signature could be used for classification of different subtypes of follicular thyroid lesions. Moreover, potential applicability of the GC/MS-based analysis of FFPE tissue samples in diagnostics of thyroid cancer has been proved. Copyright © 2015 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.

  1. The profile of selected samples of Croatian athletes based on the items of sport jealousy scale (SJS

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    Sindik Joško

    2016-01-01

    Full Text Available The role of jealousy in sport, as a negative emotional reaction, accompanied by thoughts of inadequacy when compared to others, is the issue of this article. This study had a purpose to define the characteristic profiles of the Croatian athletes, based on single items of Sport Jealousy Scale (SJS II, labeled by several variables: gender, type of sport, age group. Purposive sample of 73 athletes competing at Croatian championships in different sports (football, bowling, volleyball and handball were examined with Croatian version of SJS-II. Three clusters obtained are similarly balanced, according to the number of cases in each cluster. The most simply explained, clusters clearly differentiate the most jealous, moderately jealous and slightly/low jealous athletes. Among the features of the athletes in each cluster, in the most jealous (first cluster are the athletes from team sports, women and older athletes. Females, bowling athletes, athletes from individual (coactive sports and the youngest athletes are the least jealous (grouped in third cluster.

  2. Dark Matter Profiles in Dwarf Galaxies: A Statistical Sample Using High-Resolution Hα Velocity Fields from PCWI

    Science.gov (United States)

    Relatores, Nicole C.; Newman, Andrew B.; Simon, Joshua D.; Ellis, Richard; Truong, Phuongmai N.; Blitz, Leo

    2018-01-01

    We present high quality Hα velocity fields for a sample of nearby dwarf galaxies (log M/M⊙ = 8.4-9.8) obtained as part of the Dark Matter in Dwarf Galaxies survey. The purpose of the survey is to investigate the cusp-core discrepancy by quantifying the variation of the inner slope of the dark matter distributions of 26 dwarf galaxies, which were selected as likely to have regular kinematics. The data were obtained with the Palomar Cosmic Web Imager, located on the Hale 5m telescope. We extract rotation curves from the velocity fields and use optical and infrared photometry to model the stellar mass distribution. We model the total mass distribution as the sum of a generalized Navarro-Frenk-White dark matter halo along with the stellar and gaseous components. We present the distribution of inner dark matter density profile slopes derived from this analysis. For a subset of galaxies, we compare our results to an independent analysis based on CO observations. In future work, we will compare the scatter in inner density slopes, as well as their correlations with galaxy properties, to theoretical predictions for dark matter core creation via supernovae feedback.

  3. Profile cytological tests, sensibility and specificity of fine needle puncture into skin and subcutaneous samples in dogs.

    Directory of Open Access Journals (Sweden)

    Arlinda Flores Coleto

    2016-09-01

    Full Text Available ABSTRACT. Coleto A.F., Moreira T. de A., Gundim L.F., Silva S. de A., de Castro M. de R., Bandarra M. de B. & Medeiros-Ronchi A.A. [Profile cytological tests, sensibility and specificity of fine needle puncture into skin and subcutaneous samples in dogs.] Perfil de exames citológicos, sensibilidade e especificidade da punção por agulha fina para amostras cutâneas e subcutâneas em cães. Revista Brasileira de Medicina Veterinária, 38(3:311-315, 2016. Programa de Pós-Graduação em Ciências Veterinárias, Universidade Federal de Uberlândia, Rua Ceará s/n - Bloco 2D, Sala 3, Campus Umuarama, Uberlândia, MG 38400- 902, Brasil. E-mail: arlindacoleto@hotmail.com The cytology has been routinely used in veterinary clinic and has a good correlation with histological examination. The objective was to conduct a retrospective study of cytological examinations in the period of six years and then calculate accuracy, sensitivity and specificity of cytology examination for neoplasms in cutaneous and subcutaneous samples collected by PAF (fine needle punction in dogs, using histology as golden pattern. The canine species was the most common in 92.2% (736/798. The PAF was the most used technique in 84.0% (814/968. The most frequent samples were from the Cutaneous System in 36.1% (344/951. There was 67.0% (36/53 agreement between the exams. The sensitivity of cytology in the diagnosis of neoplasms was 77% (moderate and specificity of 12.5% (low, and the PPV 83% and NPV 9.0%. Cytology is the most commonly test used in cutaneous and subcutaneous lesions in dogs and the PAF is the most used technique. The examination is recommended as a screening method because of its high sensitivity.

  4. Infimal Convolution Regularisation Functionals of BV and Lp Spaces

    KAUST Repository

    Burger, Martin

    2016-02-03

    We study a general class of infimal convolution type regularisation functionals suitable for applications in image processing. These functionals incorporate a combination of the total variation seminorm and Lp norms. A unified well-posedness analysis is presented and a detailed study of the one-dimensional model is performed, by computing exact solutions for the corresponding denoising problem and the case p=2. Furthermore, the dependency of the regularisation properties of this infimal convolution approach to the choice of p is studied. It turns out that in the case p=2 this regulariser is equivalent to the Huber-type variant of total variation regularisation. We provide numerical examples for image decomposition as well as for image denoising. We show that our model is capable of eliminating the staircasing effect, a well-known disadvantage of total variation regularisation. Moreover as p increases we obtain almost piecewise affine reconstructions, leading also to a better preservation of hat-like structures.

  5. A fast L(p) spike alignment metric.

    Science.gov (United States)

    Dubbs, Alexander J; Seiler, Brad A; Magnasco, Marcelo O

    2010-11-01

    The metrization of the space of neural responses is an ongoing research program seeking to find natural ways to describe, in geometrical terms, the sets of possible activities in the brain. One component of this program is spike metrics-notions of distance between two spike trains recorded from a neuron. Alignment spike metrics work by identifying "equivalent" spikes in both trains. We present an alignment spike metric having L(p) underlying geometrical structure; the L(2) version is Euclidean and is suitable for further embedding in Euclidean spaces by multidimensional scaling methods or related procedures. We show how to implement a fast algorithm for the computation of this metric based on bipartite graph matching theory.

  6. 78 FR 11567 - Airworthiness Directives; Gulfstream Aerospace LP (Type Certificate Previously Held by Israel...

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    2013-02-19

    ... Aerospace LP (Type Certificate Previously Held by Israel Aircraft Industries, Ltd.) Airplanes AGENCY... Previously Held by Israel Aircraft Industries, Ltd.) Model Gulfstream G150 airplanes. This AD was prompted by...: 2013-03-23 Gulfstream Aerospace LP (Type Certificate Previously Held by Israel Aircraft Industries, Ltd...

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    2012-07-30

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    2012-10-23

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    2011-11-10

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  13. 77 FR 28375 - Eagle Rock Desoto Pipeline, L.P.; Notice of Petition for Rate Approval

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    2012-05-14

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Eagle Rock Desoto Pipeline, L.P.; Notice of Petition for Rate Approval Take notice that on May 1, 2012, Eagle Rock Desoto Pipeline, L.P. (Desoto) filed a Rate Election pursuant to...

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    2010-05-18

    ... Rock Desoto Pipeline, L.P.; Notice of Rate Election May 11, 2010. Take notice that on May 3, 2010, Eagle Rock Desoto Pipeline, L.P., (Desoto) filed a Notice of Rate Election pursuant to section 284.123(b...

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    2011-04-14

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Eagle Rock Desoto Pipeline, L.P.; Notice of Filing Take notice that on April 7, 2011, Eagle Rock Desoto Pipeline, L.P. filed a revised Statement of Operating Conditions to...

  17. On the boundedness of operators in LP(ιq and Triebel-Lizorkin Spaces

    Directory of Open Access Journals (Sweden)

    João Pedro Boto

    2008-01-01

    Full Text Available Given a bounded linear operator T:LPO(ℝn→Lp1(ℝn, we state conditions under which T defines a bounded operator between corresponding pairs of Lp(ℝn;ιq spaces and Triebel-Lizorkin spaces Fp,qs(ℝn. Applications are given to linear parabolic equations and to Schrödinger semigroups.

  18. 77 FR 74280 - Proposed Information Collection (Learner's Perception (LP) Survey); Comment Request

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    2012-12-13

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    2013-02-20

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  20. Automated Test Assembly Using lp_Solve Version 5.5 in R

    Science.gov (United States)

    Diao, Qi; van der Linden, Wim J.

    2011-01-01

    This article reviews the use of the software program lp_solve version 5.5 for solving mixed-integer automated test assembly (ATA) problems. The program is freely available under Lesser General Public License 2 (LGPL2). It can be called from the statistical language R using the lpSolveAPI interface. Three empirical problems are presented to…

  1. Automated test assembly using lp_solve version 5.5 in R

    NARCIS (Netherlands)

    Diao, Qi; van der Linden, Willem J.

    2011-01-01

    This article reviews the use of the software program lp_solve version 5.5 for solving mixed-integer automated test assembly (ATA) problems. The program is freely available under Lesser General Public License 2 (LGPL2). It can be called from the statistical language R using the lpSolveAPI interface.

  2. Specific profiles of neurocognitive and reading functions in a sample of 42 Italian boys with Duchenne Muscular Dystrophy.

    Science.gov (United States)

    Lorusso, Maria Luisa; Civati, Federica; Molteni, Massimo; Turconi, Anna Carla; Bresolin, Nereo; D'Angelo, Maria Grazia

    2013-01-01

    A group of 42 Italian boys with Duchenne Muscular Dystrophy was compared with a control group of 10 boys with Spinal Muscular Atrophy and Osteogenesis Imperfecta on tests assessing general intellectual ability, language, neuropsychological functions, and reading skills with the aim of describing a comprehensive profile of the various functions and investigating their interrelationships. The influence of general intellectual level on performance was analyzed. Further, correlations between various neuropsychological measures and language performances were computed for the group with Duchenne Muscular Dystrophy, as well as the correlations between reading scores and other cognitive and linguistic measures. A general lowering in VIQ, PIQ, and FSIQ scores was found to characterize the group with Duchenne Muscular Dystrophy. Expressive language skills were within the normal range, while syntactic and grammatical comprehension were significantly impaired. The presence of below-average reading performances was further confirmed. However, unlike previous studies on irregular orthographies, the present results show that (a) the mild reading difficulties found in the sample essentially concern speed rather than accuracy; (b) they concern word rather than nonword reading; (c) lower reading performances are related to lower scores in general IQ; (d) no correlations emerge with phonological abilities, verbal short-term memory, or working memory, but rather with long-term memory and lexical skills. This may suggest that language-specific effects modulate the cognitive expressions of Duchenne Muscular Dystrophy and raises the possibility that the dysfunctions underlying the reading difficulties observed in affected readers of regular orthographies involve different neurocognitive systems than the cortico-cerebellar circuits usually invoked.

  3. Molecular identification and antifungal susceptibility profiles of Candida parapsilosis complex species isolated from culture collection of clinical samples

    Directory of Open Access Journals (Sweden)

    Fábio Silvestre Ataides

    2015-08-01

    Full Text Available AbstractINTRODUCTION:Candida parapsilosis is a common yeast species found in cases of onychomycosis and candidemia associated with infected intravascular devices. In this study, we differentiated Candida parapsilosis sensu stricto, Candida orthopsilosis , and Candida metapsilosis from a culture collection containing blood and subungual scraping samples. Furthermore, we assessed the in vitro antifungal susceptibility of these species to fluconazole, itraconazole, voriconazole, posaconazole, amphotericin B, and caspofungin.METHODS:Differentiation of C. parapsilosis complex species was performed by amplification of the secondary alcohol dehydrogenase (SADH gene and digestion by the restriction enzyme Ban I. All isolates were evaluated for the determination of minimal inhibitory concentrations using Etest, a method for antifungal susceptibility testing.RESULTS:Among the 87 isolates, 78 (89.7% were identified as C. parapsilosis sensu stricto , five (5.7% were identified as C. orthopsilosis , and four (4.6% were identified as C. metapsilosis . Analysis of antifungal susceptibility showed that C. parapsilosis sensu strictoisolates were less susceptible to amphotericin B and itraconazole. One C. parapsilosis sensu stricto isolate was resistant to amphotericin B and itraconazole. Moreover, 10.2% of C. parapsilosis sensu stricto isolates were resistant to caspofungin. Two C. parapsilosis sensu strictoisolates and one C. metapsilosis isolate were susceptible to fluconazole in a dose-dependent manner.CONCLUSIONS:We reported the first molecular identification of C. parapsilosiscomplex species in State of Goiás, Brazil. Additionally, we showed that although the three species exhibited differences in antifungal susceptibility profiles, the primary susceptibility of this species was to caspofungin.

  4. Microdialysis Sampling from Wound Fluids Enables Quantitative Assessment of Cytokines, Proteins, and Metabolites Reveals Bone Defect-Specific Molecular Profiles.

    Science.gov (United States)

    Förster, Yvonne; Schmidt, Johannes R; Wissenbach, Dirk K; Pfeiffer, Susanne E M; Baumann, Sven; Hofbauer, Lorenz C; von Bergen, Martin; Kalkhof, Stefan; Rammelt, Stefan

    2016-01-01

    Bone healing involves a variety of different cell types and biological processes. Although certain key molecules have been identified, the molecular interactions of the healing progress are not completely understood. Moreover, a clinical routine for predicting the quality of bone healing after a fracture in an early phase is missing. This is mainly due to a lack of techniques to comprehensively screen for cytokines, growth factors and metabolites at their local site of action. Since all soluble molecules of interest are present in the fracture hematoma, its in-depth assessment could reveal potential markers for the monitoring of bone healing. Here, we describe an approach for sampling and quantification of cytokines and metabolites by using microdialysis, combined with solid phase extractions of proteins from wound fluids. By using a control group with an isolated soft tissue wound, we could reveal several bone defect-specific molecular features. In bone defect dialysates the neutrophil chemoattractants CXCL1, CXCL2 and CXCL3 were quantified with either a higher or earlier response compared to dialysate from soft tissue wound. Moreover, by analyzing downstream adaptions of the cells on protein level and focusing on early immune response, several proteins involved in the immune cell migration and activity could be identified to be specific for the bone defect group, e.g. immune modulators, proteases and their corresponding inhibitors. Additionally, the metabolite screening revealed different profiles between the bone defect group and the control group. In summary, we identified potential biomarkers to indicate imbalanced healing progress on all levels of analysis.

  5. Microdialysis Sampling from Wound Fluids Enables Quantitative Assessment of Cytokines, Proteins, and Metabolites Reveals Bone Defect-Specific Molecular Profiles.

    Directory of Open Access Journals (Sweden)

    Yvonne Förster

    Full Text Available Bone healing involves a variety of different cell types and biological processes. Although certain key molecules have been identified, the molecular interactions of the healing progress are not completely understood. Moreover, a clinical routine for predicting the quality of bone healing after a fracture in an early phase is missing. This is mainly due to a lack of techniques to comprehensively screen for cytokines, growth factors and metabolites at their local site of action. Since all soluble molecules of interest are present in the fracture hematoma, its in-depth assessment could reveal potential markers for the monitoring of bone healing. Here, we describe an approach for sampling and quantification of cytokines and metabolites by using microdialysis, combined with solid phase extractions of proteins from wound fluids. By using a control group with an isolated soft tissue wound, we could reveal several bone defect-specific molecular features. In bone defect dialysates the neutrophil chemoattractants CXCL1, CXCL2 and CXCL3 were quantified with either a higher or earlier response compared to dialysate from soft tissue wound. Moreover, by analyzing downstream adaptions of the cells on protein level and focusing on early immune response, several proteins involved in the immune cell migration and activity could be identified to be specific for the bone defect group, e.g. immune modulators, proteases and their corresponding inhibitors. Additionally, the metabolite screening revealed different profiles between the bone defect group and the control group. In summary, we identified potential biomarkers to indicate imbalanced healing progress on all levels of analysis.

  6. The runaway binary LP 400-22 is leaving the Galaxy

    Science.gov (United States)

    Kilic, Mukremin; Gianninas, A.; Brown, Warren R.; Harris, Hugh C.; Dahn, Conard C.; Agüeros, M. A.; Heinke, Craig O.; Kenyon, S. J.; Panei, J. A.; Camilo, Fernando

    2013-10-01

    We present optical spectroscopy, astrometry, radio and X-ray observations of the runaway binary LP 400-22. We refine the orbital parameters of the system based on our new radial velocity observations. Our parallax data indicate that LP 400-22 is significantly more distant (3σ lower limit of 840 pc) than initially predicted. LP 400-22 has a tangential velocity in excess of 830 km s-1; it is unbound to the Galaxy. Our radio and X-ray observations fail to detect a recycled millisecond pulsar companion, indicating that LP 400-22 is a double white dwarf system. This essentially rules out a supernova runaway ejection mechanism. Based on its orbit, a Galactic Centre origin is also unlikely. However, its orbit intersects the locations of several globular clusters; dynamical interactions between LP 400-22 and other binary stars or a central black hole in a dense cluster could explain the origin of this unusual binary.

  7. Passive Sampling and High Resolution Mass Spectrometry for Chemical Profiling of French Coastal Areas with a Focus on Marine Biotoxins.

    Science.gov (United States)

    Zendong, Zita; Bertrand, Samuel; Herrenknecht, Christine; Abadie, Eric; Jauzein, Cécile; Lemée, Rodolphe; Gouriou, Jérémie; Amzil, Zouher; Hess, Philipp

    2016-08-16

    Passive samplers (solid phase adsorption toxin tracking: SPATT) are able to accumulate biotoxins produced by microalgae directly from seawater, thus providing useful information for monitoring of the marine environment. SPATTs containing 0.3, 3, and 10 g of resin were deployed at four different coastal areas in France and analyzed using liquid chromatography coupled to high resolution mass spectrometry. Quantitative targeted screening provided insights into toxin profiles and showed that toxin concentrations and profiles in SPATTs were dependent on the amount of resin used. Between the three amounts of resin tested, SPATTs containing 3 g of resin appeared to be the best compromise, which is consistent with the use of 3 g of resin in SPATTs by previous studies. MassHunter and Mass Profiler Professional softwares were used for data reprocessing and statistical analyses. A differential profiling approach was developed to investigate and compare the overall chemical diversity of dissolved substances in different coastal water bodies. Principal component analysis (PCA) allowed for spatial differentiation between areas. Similarly, SPATTs retrieved from the same location at early, medium, and late deployment periods were also differentiated by PCA, reflecting seasonal variations in chemical profiles and in the microalgal community. This study used an untargeted metabolomic approach for spatial and temporal differentiation of marine environmental chemical profiles using SPATTs, and we propose this approach as a step forward in the discovery of chemical markers of short- or long-term changes in the microbial community structure.

  8. Primary health clinic toilet/bathroom surface swab sampling can indicate community profile of sexually transmitted infections.

    Science.gov (United States)

    Giffard, Philip M; Su, Jiunn-Yih; Andersson, Patiyan; Holt, Deborah C

    2017-01-01

    The microbiome of built environment surfaces is impacted by the presence of humans. In this study, we tested the hypothesis that analysis of surface swabs from clinic toilet/bathroom yields results correlated with sexually transmitted infection (STI) notifications from corresponding human populations. We extended a previously reported study in which surfaces in toilet/bathroom facilities in primary health clinics in the Australian Northern Territory (NT) were swabbed then tested for nucleic acid from the STI agents Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis. This was in the context of assessing the potential for such nucleic acid to contaminate specimens collected in such facilities. STIs are notifiable in the NT, thus allowing comparison of swab and notification data. An assumption in the design was that while absolute built environment loads of STI nucleic acids will be a function of patient traffic density and facility cleaning protocols, the relative loads of STI nucleic acids from different species will be largely unaffected by these processes. Another assumption was that the proportion of swabs testing positive for STIs provides a measure of surface contamination. Accordingly, "STI profiles" were calculated. These were the proportions that each of the three STIs of interest contributed to the summed STI positive swabs or notifications. Three comparisons were performed, using swab data from clinics in remote Indigenous communities, clinics in small-medium towns, and a single urban sexual health clinic. These data were compared with time and place-matched STI notifications. There were significant correlations between swab and notifications data for the both the remote Indigenous and regional data. For the remote Indigenous clinics the p values ranged from 0.041 to 0.0089, depending on data transformation and p value inference method. Further, the swab data appeared to strongly indicate known higher relative prevalence of gonorrhoeae

  9. Mode division multiplexing coupler of four LP modes based on a five-core microstructured optical fiber

    Science.gov (United States)

    Zhang, Hongwei; Liu, Yange; Wang, Zhi; Han, Ya; Yang, Kang; Yu, Jie

    2018-03-01

    An all-fiber mode division multiplexing coupler based on a five-core microstructured optical fiber is proposed to multiplex or demultiplex LP11, LP21, LP02 and LP01 modes simultaneously. The five-core microstructured optical fiber is composed of a few mode core supporting the four LP modes propagating at 1550 nm and four single-mode cores surrounding the few-mode core. The coupling mechanism between LP01 mode in each single-mode core and the corresponding four LP modes in the few-mode core is based on avoid-crossing effect. Numerical simulation results show that coupling bandwidths of LP11, LP21, LP02 and LP01 mode are 115 nm, 56 nm, 30 nm, 46 nm respectively, with a coupling length of 2156.1 μm. The proposed mode division multiplexing coupler can multiplex or demultiplex four LP modes simultaneously with large operation bandwidth, making it a good candidate for space-division multiplexing system.

  10. 77 FR 76586 - Founders Equity SBIC I, L.P.; Notice Seeking Exemption Under Section 312 of the Small Business...

    Science.gov (United States)

    2012-12-28

    ... Doc No: 2012-31325] SMALL BUSINESS ADMINISTRATION [License No. 02/72-0625] Founders Equity SBIC I, L.P... Notice is hereby given that Founders Equity SBIC I, L.P., 711 Fifth Avenue, 5th Floor, New York, NY 10022... (``SBA'') Rules and Regulations (13 CFR 107.730). Founders Equity SBIC I, L.P. proposes to provide debt...

  11. 78 FR 57444 - Eagle Fund III, L.P.; Notice Seeking Exemption Under the Small Business Investment Act, Conflicts...

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    2013-09-18

    ... ADMINISTRATION Eagle Fund III, L.P.; Notice Seeking Exemption Under the Small Business Investment Act, Conflicts of Interest Notice is hereby given that Eagle Fund III, L.P., 101 S. Hanley Road, Suite 1250, St... Business Administration (``SBA'') Rules and Regulations. Eagle Fund III, L.P., provided debt and equity...

  12. 78 FR 57444 - Eagle Fund III-A, L.P.; Notice Seeking Exemption Under the Small Business Investment Act...

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    2013-09-18

    ... ADMINISTRATION Eagle Fund III-A, L.P.; Notice Seeking Exemption Under the Small Business Investment Act, Conflicts of Interest Notice is hereby given that Eagle Fund III-A, L.P., 101 S. Hanley Road, Suite 1250, St... Business Administration (``SBA'') Rules and Regulations. Eagle Fund III-A, L.P., provided debt and equity...

  13. 76 FR 59767 - Plexus Fund II, L.P.; Notice Seeking Exemption Under Section 312 of the Small Business Investment...

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    2011-09-27

    ... ADMINISTRATION Plexus Fund II, L.P.; Notice Seeking Exemption Under Section 312 of the Small Business Investment Act, Conflicts of Interest Notice is hereby given that Plexus Fund II, L.P., 200 Providence Road... Business Administration (``SBA'') Rules and Regulations (13 CFR 107.730). Plexus II, L.P., proposes to...

  14. Routine use of microarray-based gene expression profiling to identify patients with low cytogenetic risk acute myeloid leukemia: accurate results can be obtained even with suboptimal samples

    Directory of Open Access Journals (Sweden)

    de la Blétière Diane Raingeard

    2012-01-01

    Full Text Available Abstract Background Gene expression profiling has shown its ability to identify with high accuracy low cytogenetic risk acute myeloid leukemia such as acute promyelocytic leukemia and leukemias with t(8;21 or inv(16. The aim of this gene expression profiling study was to evaluate to what extent suboptimal samples with low leukemic blast load (range, 2-59% and/or poor quality control criteria could also be correctly identified. Methods Specific signatures were first defined so that all 71 acute promyelocytic leukemia, leukemia with t(8;21 or inv(16-AML as well as cytogenetically normal acute myeloid leukemia samples with at least 60% blasts and good quality control criteria were correctly classified (training set. The classifiers were then evaluated for their ability to assign to the expected class 111 samples considered as suboptimal because of a low leukemic blast load (n = 101 and/or poor quality control criteria (n = 10 (test set. Results With 10-marker classifiers, all training set samples as well as 97 of the 101 test samples with a low blast load, and all 10 samples with poor quality control criteria were correctly classified. Regarding test set samples, the overall error rate of the class prediction was below 4 percent, even though the leukemic blast load was as low as 2%. Sensitivity, specificity, negative and positive predictive values of the class assignments ranged from 91% to 100%. Of note, for acute promyelocytic leukemia and leukemias with t(8;21 or inv(16, the confidence level of the class assignment was influenced by the leukemic blast load. Conclusion Gene expression profiling and a supervised method requiring 10-marker classifiers enable the identification of favorable cytogenetic risk acute myeloid leukemia even when samples contain low leukemic blast loads or display poor quality control criterion.

  15. Optical manipulation of biological particles using LP21 mode in fiber

    Science.gov (United States)

    Chen, Shijie; Huang, He; Zou, Hongmei; Li, Qing; Fu, Jian; Lin, Feng; Wu, X.

    2014-12-01

    We demonstrate the optical manipulation of biological particles using a low-order LP21 fiber mode. The focused four-lobed LP21 mode distribution was theoretically and experimentally found to be effective in optical tweezer applications, including selective cellular pick-up, pairing, grouping or separation, as well as rotation of cell dimers and clusters. Our proposed theoretical model estimates both the translational dragging force and rotational torque in good accordance with experimental data. With a simple all-fiber configuration, and low peak irradiation to target bioparticles, the proposed LP21 ‘optical chuck’ system has great application potential in biological test systems.

  16. The maximal operator in weighted variable spaces Lp(⋅

    Directory of Open Access Journals (Sweden)

    Vakhtang Kokilashvili

    2007-01-01

    Full Text Available We study the boundedness of the maximal operator in the weighted spaces Lp(⋅(ρ over a bounded open set Ω in the Euclidean space ℝn or a Carleson curve Γ in a complex plane. The weight function may belong to a certain version of a general Muckenhoupt-type condition, which is narrower than the expected Muckenhoupt condition for variable exponent, but coincides with the usual Muckenhoupt class Ap in the case of constant p. In the case of Carleson curves there is also considered another class of weights of radial type of the form ρ(t=∏k=1mwk(|t-tk|, tk∈Γ, where wk has the property that r1p(tkwk(r∈Φ10, where Φ10 is a certain Zygmund-Bari-Stechkin-type class. It is assumed that the exponent p(t satisfies the Dini–Lipschitz condition. For such radial type weights the final statement on the boundedness is given in terms of the index numbers of the functions wk (similar in a sense to the Boyd indices for the Young functions defining Orlich spaces.

  17. Social Media Membership, Browsing, and Profile Updating in a Representative U.S. Sample: Independent and Interdependent Effects of Big Five Traits and Aging and Social Factors

    Science.gov (United States)

    Bogg, Tim

    2017-01-01

    Guided by cybernetic perspectives on personality, the present work used a representative sample of U.S. adults (N = 992) to examine Big Five personality traits and social and aging factors as predictors of social media network membership and past-month browsing/searching and profile updating among members. The results showed adults who were less extraverted and less neurotic and who reported greater physical limitations were less likely to be members. Moreover, extraverted adults without partners were more likely to be members than introverted adults without partners. Among members, the results showed extraverted and emotionally stable younger and older adults reported a similar frequency of profile updating. In contrast, older adults with all other combinations of extraversion and neuroticism showed a reduced frequency of profile updating compared to younger adults. The findings are discussed in terms of social media involvement as a response of a self-regulatory system of personality adaptation. PMID:28713320

  18. Social Media Membership, Browsing, and Profile Updating in a Representative U.S. Sample: Independent and Interdependent Effects of Big Five Traits and Aging and Social Factors.

    Science.gov (United States)

    Bogg, Tim

    2017-01-01

    Guided by cybernetic perspectives on personality, the present work used a representative sample of U.S. adults ( N = 992) to examine Big Five personality traits and social and aging factors as predictors of social media network membership and past-month browsing/searching and profile updating among members. The results showed adults who were less extraverted and less neurotic and who reported greater physical limitations were less likely to be members. Moreover, extraverted adults without partners were more likely to be members than introverted adults without partners. Among members, the results showed extraverted and emotionally stable younger and older adults reported a similar frequency of profile updating. In contrast, older adults with all other combinations of extraversion and neuroticism showed a reduced frequency of profile updating compared to younger adults. The findings are discussed in terms of social media involvement as a response of a self-regulatory system of personality adaptation.

  19. Culture and Validity of the Symptom Checklist-90-Revised and Profile of Mood States in a New Zealand Student Sample.

    Science.gov (United States)

    Barker-Collo, Suzanne L.

    2003-01-01

    New Zealand students' performance was examined on assessments of psychopathology and mood as compared to normative data from the United States. New Zealand university students (N=137) completed the Symptom Checklist-90-Revised (SCL-90-R) and Profile of Mood States (POMS). Mean performances differed significantly from normative data for each…

  20. An LC-MS/MS method for steroid profiling during adrenal venous sampling for investigation of primary aldosteronism

    NARCIS (Netherlands)

    Peitzsch, M.; Dekkers, T.; Haase, M.; Sweep, F.C.; Quack, I.; Antoch, G.; Siegert, G.; Lenders, J.W.M.; Deinum, J.; Willenberg, H.S.; Eisenhofer, G.

    2015-01-01

    BACKGROUND: Steroid profiling for diagnosis of endocrine disorders featuring disordered production of steroid hormones is now possible from advances in liquid chromatography with tandem mass spectrometry (LC-MS/MS). Adrenal venous (AV) measurements of aldosterone and cortisol are a standard practice

  1. Somatoform symptoms profiles in relation to psychological disorders - A population classification analysis in a large sample of general adults.

    Science.gov (United States)

    Heidari, Zahra; Feizi, Awat; Roohafza, Hamidreza; Hassanzadeh Keshteli, Ammar; Adibi, Payman

    2017-08-01

    In order to identifying somatoform symptoms profiles, classifying study population and evaluating of psychological disorders in extracted classes, we carried out a cross-sectional study on 4762 Iranian adults. Somatoform symptoms were assessed using a comprehensive 30-items questionnaire and psychological disorders were evaluated by 12-item General Health Questionnaire (GHQ-12) and Hospital Anxiety and Depression Scale (HADS) questionnaires. Factor analysis and factor mixture modeling (FMM) were used for data analysis. Four somatoform symptoms profiles were extracted, including 'psycho-fatigue', 'gastrointestinal', 'neuro- skeletal' and 'pharyngeal-respiratory'. According to FMM results, a two-class four-factor structure, based somatoform symptoms, was identified in our study population. Two identified classes were labeled as "low psycho-fatigue complaints" and "high psycho-fatigue complaints". The scores of psychological disorders profile was significantly associated with four somatoform symptoms profiles in both classes; however the stronger relationship was observed in high psycho-fatigue complaints class. The prevalence of all the somatoform symptoms among participants assigned to the "high psycho-fatigue complaints" class was significantly higher than other class. We concluded that somatoform symptoms have a dimensional-categorical structure within our study population. Our study also provided informative pathways on the association of psychological disorders with somatoform symptoms. These findings could be useful for dealing with treatment's approaches. Copyright © 2017. Published by Elsevier B.V.

  2. Robust BRCA1-like classification of copy number profiles of samples repeated across different datasets and platforms

    NARCIS (Netherlands)

    Schouten, Philip C.; Grigoriadis, Anita; Kuilman, Thomas; Mirza, Hasan; Watkins, Johnathan A.; Cooke, Saskia A.; van Dyk, Ewald; Severson, Tesa M.; Rueda, Oscar M.; Hoogstraat, Marlous; Verhagen, Caroline V. M.; Natrajan, Rachael; Chin, Suet-Feung; Lips, Esther H.; Kruizinga, Janneke; Velds, Arno; Nieuwland, Marja; Kerkhoven, Ron M.; Krijgsman, Oscar; Vens, Conchita; Peeper, Daniel; Nederlof, Petra M.; Caldas, Carlos; Tutt, Andrew N.; Wessels, Lodewyk F.; Linn, Sabine C.

    Breast cancers with BRCA1 germline mutation have a characteristic DNA copy number (CN) pattern. We developed a test that assigns CN profiles to be 'BRCA1-like' or 'non-BRCA1-like', which refers to resembling a BRCA1-mutated tumor or resembling a tumor without a BRCA1 mutation, respectively.

  3. Role of the beam profile and sample geometry in the bonded-disk method on the shrinkage kinetics of two dental resins.

    Science.gov (United States)

    Kostylev, I; Winters, T; Labrie, D; Price, R B T

    2016-08-01

    To investigate the time evolution of the two dimensional axial shrinkage field for two dental resins in the bonded disk geometry and further test the bonded-disk method. An interferometric technique employing a camera was used to image the 2D axial shrinkage field when polymerizing dental resins in real time both during and after light exposure. Four different beam profiles from two light curing units and three sample geometries were utilized to investigate their roles on the 2D axial shrinkage field. The 2D axial shrinkage field correlates qualitatively with the beam profile shortly after the start of light exposure but takes on distinct shapes caused by the rigidity of the coverslip, beam profile, and the resin viscoelasticity. Using the conventional bonded disk geometry and uniform beam profile from a light curing unit, the axial 2D shrinkage field was uniform to within 4% in the central part of bonded disk samples. Copyright © 2016 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

  4. Lipid profile and lipoprotein(a) in chronic renal failure patients with and without hemodialysis

    OpenAIRE

    Hariom Sharma; Tejas J Shah; Jignesh H Gorasia; Dipika P Baria

    2012-01-01

    Objectives: Chronic renal failure (CRF) is complicated by characteristic dyslipidemias. CRF patients on hemodialysis have abnormalities in lipid profile and have a high incidence of cardiovascular diseases. Lipoprotein(a) [Lp(a)] is now considered as a novel cardiovascular risk factor and its level is increased in CRF patients with and without hemodialysis. We sought to evaluate the pattern of lipid profile including Lp(a) level in CRF patients with and without hemodialysis. Methodol...

  5. Partial pressure (or fugacity) of carbon dioxide, dissolved inorganic carbon, temperature, salinity and other variables collected from discrete sample, profile and time series profile observations using CTD, bottle and other instruments from ARNI FRIDRIKSSON and BJARNI SAEMUNDSSON in the North Greenland Sea from 1991-08-15 to 2006-10-02 (NODC Accession 0100063)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0100063 includes chemical, discrete sample, physical, profile and time series profile data collected from ARNI FRIDRIKSSON and BJARNI SAEMUNDSSON in...

  6. Partial pressure (or fugacity) of carbon dioxide, dissolved inorganic carbon, temperature, salinity and other variables collected from discrete sample, profile and time series profile observations using CTD, bottle and other instruments from ARNI FRIDRIKSSON and BJARNI SAEMUNDSSON in the North Atlantic Ocean from 1991-08-08 to 2006-02-02 (NODC Accession 0100114)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0100114 includes chemical, discrete sample, physical, profile and time series profile data collected from ARNI FRIDRIKSSON and BJARNI SAEMUNDSSON in...

  7. Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample, profile and time series profile observations using CTD, bottle and other instruments from LA CURIEUSE in the Indian Ocean from 1990-01-27 to 1995-01-08 (NODC Accession 0112882)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0112882 includes biological, chemical, discrete sample, physical, profile and time series profile data collected from LA CURIEUSE in the Indian Ocean...

  8. Partial pressure (or fugacity) of carbon dioxide, dissolved inorganic carbon, pH, alkalinity, temperature, salinity and other variables collected from discrete sample, profile and time series profile observations using Alkalinity titrator, CTD and other instruments from HERMANO GINES in the Caribbean Sea from 1995-11-08 to 2015-07-29 (NODC Accession 0112926)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0112926 includes discrete sample, profile and time series profile data collected from HERMANO GINES in the Caribbean Sea from 1995-11-08 to...

  9. Dissolved inorganic carbon, alkalinity, temperature, salinity and other variables collected from discrete sample, profile and time series profile observations using Alkalinity titrator, CTD and other instruments from POLARFRONT in the Norwegian Sea from 2001-10-31 to 2007-11-29 (NODC Accession 0112884)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0112884 includes chemical, discrete sample, physical, profile and time series profile data collected from POLARFRONT in the Norwegian Sea from...

  10. DETERMINATION OF LEVEL OF FOOD ADDITIVES IN Labisia pumila (LP BEVERAGES CONSUMED IN KUANTAN, MALAYSIA

    Directory of Open Access Journals (Sweden)

    Ade Chandra Iwansyah

    2013-03-01

    Full Text Available The content levels of several food additives (gallic acid, benzoic acid and caffeine in commercial Labisia pumila (LP beverage samples in Kuantan, Malaysia were determined by high performances liquid chromatography (HPLC. These analytical measurements were undertaken primarily to assess the compliance of content levels of the investigated food additives and their daily intake doses with permissible levels. The results obtained from this study indicated that the average levels of GA, caffeine and benzoic acid in the analyzed beverages were 37.62-229.35 ppm, 43.46 -168.00 ppm and 98.10-241.13 ppm, respectively. In addition, the concentrations of these food additives have been converted into daily intake doses based on beverage consumption. It was estimated that the mean daily intake of GA, caffeine and benzoic acid  by the adult population of Kuantan through the consumption of the analyzed beverages  were 0.39 mg/kg body weight/day for GA, 0.59 mg/kg body weight/day for caffeine (19.6 % ADI and 0.43 mg/kg body weight/day for benzoic acid (8.6%ADI. None of the analyzed beverage sample was found to violate the current legal limits as stipulated in Malaysian food regulation.   Keywords: beverages, daily intake, food additives, Labisia pumila

  11. LP-tee pikeneb Tartust Pärnuni / Sirje Pärismaa

    Index Scriptorium Estoniae

    Pärismaa, Sirje, 1958-

    2015-01-01

    Norrast pärit tõenduspõhist koostöökultuuri arendava LP-mudeliga (learning environment and pedagogical analysis) on liitumas seitse kooli Pärnust, Viljandist, Puhjast, Tartust ning kolm lasteaeda

  12. LibraryH3lp: A New Flexible Chat Reference System

    Directory of Open Access Journals (Sweden)

    Pam Sessoms

    2008-09-01

    Full Text Available LibraryH3lp is an integrated IM and web chat system designed specifically for Virtual Reference services in libraries. The software was designed for, and is currently used by, a night-time chat reference collaboraton between several large academic libraries. LibraryH3lp is designed for the workflow of chat reference, supporting multiple simultaneous operators and routing to queues of operators in a particular service area. It also supports web page embeddable chat 'widgets', as well as simultaneous gateways to multiple IM protocols. This article discusses the motivation for the development of the software, and provides an overview of LibraryH3lp's features and technical architecture. Parts of LibraryH3lp are available as open source. The complete application is available as a low-cost hosted service, and will eventually be available to be licensed for local hosting.

  13. Characterization of LpGPAT Gene in Lilium pensylvanicum and Response to Cold Stress

    Directory of Open Access Journals (Sweden)

    Shao-kun Sun

    2015-01-01

    Full Text Available LpGPAT was obtained from L. pensylvanicum using RT-PCR and rapid amplification of cDNA ends. The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46 KDa. The nucleic acid sequence analysis showed that it shared high homology with other known GPATs. SMAT result suggests that there is a PlsC that exists in 176-322 amino acid sequence of LpGAPT; it means LpGPAT protein is a member of the family of acyltransferase and has acyltransferase enzymatic activity. Result of real-time quantitative PCR and semiquantitative PCR support LpGPAT gene is definitely induced by low temperature stress.

  14. LP MOON GAMMA RAY SPECTROMETER 3 RDR V1.0

    Data.gov (United States)

    National Aeronautics and Space Administration — This data set contains processed LP-GRS time-series data that are used as the fundamental data for deriving composition measurements of the lunar surface using...

  15. Consumer-based product characterization using Pivot Profile, Projective Mapping and Check-all-that-apply (CATA): A comparative case with Greek yogurt samples.

    Science.gov (United States)

    Esmerino, Erick A; Tavares Filho, Elson R; Thomas Carr, B; Ferraz, Juliana P; Silva, Hugo L A; Pinto, Letícia P F; Freitas, Mônica Q; Cruz, Adriano G; Bolini, Helena M A

    2017-09-01

    Product characterization has been a primary concern for the food industry, and methodologies based on consumers' perceptions have become popular and widely used by industries to replace classical methods. Although there are several studies on other methods, the potential of reference-based one such as Pivot Profile is still little explored. Therefore, the aims of this study were to characterize Greek yogurt samples according to consumers' perceptions using three different methodologies: Pivot Profile (PP), Check-all-that-apply (CATA), and Projective Mapping (PM), and to assess which method is easier for consumers to describe products. The rapid methodologies assessed were equally effective in characterizing the different samples; however, some drawbacks evidenced in the study can help in targeting and choosing the best method to perform the sensory characterization. Pivot Profile showed some advantages, bypassing some limitations presented by the other methods. In addition, its experimental versatility also allows for broad applications evidencing the PP technique as a promising tool for routine use. Some implications of using it were also discussed. We suggest the supplemental use of Multidimensional Alignment (MDA) as it shows more accurately the correlations between attributes and samples, especially in the case of PP data. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Metabolite profiling on wheat grain to enable a distinction of samples from organic and conventional farming systems

    OpenAIRE

    Bonte, Anja; Neuweger, Heiko; Goesmann, Alexander; Thonar, Cécile; Mäder, Paul; Langenkämper, Georg; Niehaus, Karsten

    2014-01-01

    Identification of biomarkers capable of distinguishing organic and conventional products would be highly welcome to improve the strength of food quality assurance. Metabolite profiling was used for biomarker search in organic and conventional wheat grain (Triticum aestivum L.) of 11 different old and new bread wheat cultivars grown in the DOK system comparison trial. Metabolites were extracted usingmethanol and analysed by gas chromatography–mass spectrometry.

  17. Latent homeless risk profiles of a national sample of homeless veterans and their relation to program referral and admission patterns.

    Science.gov (United States)

    Tsai, Jack; Kasprow, Wesley J; Rosenheck, Robert A

    2013-12-01

    We identified risk and need profiles of homeless veterans and examined the relation between profiles and referrals and admissions to Department of Veterans Affairs (VA) homeless service programs. We examined data from the VA's new Homeless Operations Management and Evaluation System on 120,852 veterans from 142 sites nationally in 2011 and 2012 using latent class analyses based on 9 homeless risk factors. The final 4-class solution compared both referral and admission to VA homeless services. We identified 4 latent classes: relatively few problems, dual diagnosis, poverty-substance abuse-incarceration, and disabling medical problems. Homeless veterans in the first group were more likely to be admitted to the VA's permanent supportive housing program, whereas those in the second group were more likely to be admitted to more restrictive VA residential treatment. Homeless veterans in the third group were more likely to be admitted to the VA's prisoner re-entry program, and those in the fourth group were more likely to be directed to VA medical services. The heterogeneous risk and need profiles of homeless veterans supported the diversity of VA homeless services and encouraged the development of specialized services to meet their diverse needs.

  18. Assessing mental health in boys with Duchenne muscular dystrophy: Emotional, behavioural and neurodevelopmental profile in an Italian clinical sample.

    Science.gov (United States)

    Colombo, Paola; Nobile, Maria; Tesei, Alessandra; Civati, Federica; Gandossini, Sandra; Mani, Elisa; Molteni, Massimo; Bresolin, Nereo; D'Angelo, Grazia

    2017-07-01

    To evaluate through a comprehensive protocol, the psychopathological profile of DMD boys. The primary aim of this observational study was to describe the emotional and behavioural profile and the neurodevelopmental problems of Italian boys with Duchenne Muscular Dystrophy (DMD); the secondary aim was to explore the relation between psychopathological profile and DMD genotype. 47 DMD boys, aged 2-18, were included in the study and assessed through structured and validated tools including Wechsler scales or Griffiths for cognitive ability, Child Behavior Check List (CBCL), Youth Self Report (YSR) and Strengths and Difficulties Questionnaire (SDQ) for emotional and behavioural features. Patients "at risk" based on questionnaires scores were evaluated by a clinical structured interview using Development and Well Being Assessment (DAWBA) or Autism Diagnostic Observation Schedule (ADOS), as required. The 47 enrolled patients, defined with a Full Scale Intelligence Quotient (FSIQ) of 80.38 (one SD below average), and presenting a large and significant difference in FSIQ in relation to the site of mutation along the dystrophin gene (distal mutations associated with a more severe cognitive deficit), were showing Internalizing Problems (23.4%) and Autism Spectrum Disorders (14.8%). Interestingly, an association of internalizing problems with distal deletion of the DMD gene is documented. Even though preliminary, these data show that the use of validated clinical instruments, that focus on the impact of emotional/behaviour problems on everyday life, allows to carefully identify clinically significant psychopathology. Copyright © 2017 European Paediatric Neurology Society. Published by Elsevier Ltd. All rights reserved.

  19. HT-LP thermometamorphism modelling : Agly massif, French Pyrenees.

    Science.gov (United States)

    Tournaire Guille, Baptiste; Pascal, Marie-Lola; Lejeune, Anne-Marie; Annen, Catherine

    2017-04-01

    Owing to the strongly anomalous thermal gradients implied, HT-LP metamorphism is a worldwide type of processes in which magma emplacement and solidification at relatively high levels in the crust must be considered as a potentially major heat source. Thermal modelling (e.g. Annen et al. 2005) is an appropriate tool for constraining the part played by such processes in practical cases of thermometamorphism. We study the Agly massif, an exhumed part of middle crust from the Variscan belt in the French Pyrenees. This massif is a classical example of HT-LP metamorphism (Vielzeuf 1996), composed of a metasedimentary cover, mainly micaschists aged from upper Cambrian to Devonian, unconformably overlying an older basement of para- and orthogneisses. The Variscan metamorphic facies extend from greenschists, in the upper part of the cover, to granulites in the basement (Fonteilles 1976). The apparent geotherm of about 110°/km in the metasedimentary cover (amphibolite and greenschist facies) has given way to contrasting interpretations. Magmatic activity partly synchronous with and probably related to the Variscan thermometamorphism is observed at the outcropping level as at least 4 magmatic bodies of mantle origin (Touil 1994), of Stephanian age, including granodiorites and subordinate diorites and gabbros. Recent U/Pb datations on zircons (Tournaire-Guille et al., in prep) also reveal the presence of lower Cambrian magmatism in the gneisses, therefore confirming their interpretation as a pre-Variscan basement. The location (depth), volume (thickness), temperature (composition) and timing of magma emplacement are the parameters controlling the thermal effect to be modelled with a Matlab® code (Annen et al. 2005). In order to constrain these parameters, we have updated the lithostratigraphy and the PT conditions of the Variscan metamorphism in the Agly area. Mineralogic and petrologic data exploited in thermobarometric analyses compared with thermodynamic PerpleX modelling

  20. Elevated inflammatory Lp-PLA2 and IL-6 link e-waste Pb toxicity to cardiovascular risk factors in preschool children.

    Science.gov (United States)

    Lu, Xueling; Xu, Xijin; Zhang, Yu; Zhang, Yuling; Wang, Chenyang; Huo, Xia

    2018-03-01

    Cardiovascular toxicity of lead (Pb) manifests primarily as an effect on blood pressure and eventual increased risk of atherosclerosis and cardiovascular events. Therefore, we investigated vascular inflammatory biomarkers and cardiovascular effects of Pb-exposed children. A total of 590 children (3-7 years old) were recruited from Guiyu (n = 337), an electronic waste (e-waste)-exposed group, and Haojiang (n = 253), a reference group, from November to December 2016. We measured child blood Pb levels (BPbs), and systolic and diastolic blood pressure. Pulse pressure was calculated for the latter two. Serum biomarkers including lipid profiles and inflammatory cytokines, and plasma lipoprotein-associated phospholipase A2 (Lp-PLA2) were detected. Unadjusted regression analysis illustrated that higher ln-transformed BPb associated with lower systolic blood pressure and pulse pressure. After adjustment for various confounders, the relational degree of lnBPb and blood pressure measures became slightly attenuated or not significant. Elevated BPb was associated with higher Lp-PLA2, interleukin (IL)-6, triglycerides (TG) and lower high-density lipoprotein (HDL). Lp-PLA2 remained inversely associated with pulse pressure and HDL, but positively with ratios of total cholesterol to HDL (Tc/HDL) and low-density lipoprotein to HDL (LDL/HDL). IL-6 was associated negatively with systolic blood pressure, pulse pressure and HDL, and positively associated with TG, Tc/HDL and LDL/HDL. The mediation effect of biomarkers on the association of BPb with pulse pressure was insignificant except for Lp-PLA2. Available data supports the conclusion that e-waste-exposed children with higher BPbs and concomitant abnormal measures of cardiovascular physiology have an augmented prevalence of vascular inflammation, as well as lipid disorder. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Optimal protein extraction methods from diverse sample types for protein profiling by using Two-Dimensional Electrophoresis (2DE).

    Science.gov (United States)

    Tan, A A; Azman, S N; Abdul Rani, N R; Kua, B C; Sasidharan, S; Kiew, L V; Othman, N; Noordin, R; Chen, Y

    2011-12-01

    There is a great diversity of protein samples types and origins, therefore the optimal procedure for each sample type must be determined empirically. In order to obtain a reproducible and complete sample presentation which view as many proteins as possible on the desired 2DE gel, it is critical to perform additional sample preparation steps to improve the quality of the final results, yet without selectively losing the proteins. To address this, we developed a general method that is suitable for diverse sample types based on phenolchloroform extraction method (represented by TRI reagent). This method was found to yield good results when used to analyze human breast cancer cell line (MCF-7), Vibrio cholerae, Cryptocaryon irritans cyst and liver abscess fat tissue. These types represent cell line, bacteria, parasite cyst and pus respectively. For each type of samples, several attempts were made to methodically compare protein isolation methods using TRI-reagent Kit, EasyBlue Kit, PRO-PREP™ Protein Extraction Solution and lysis buffer. The most useful protocol allows the extraction and separation of a wide diversity of protein samples that is reproducible among repeated experiments. Our results demonstrated that the modified TRI-reagent Kit had the highest protein yield as well as the greatest number of total proteins spots count for all type of samples. Distinctive differences in spot patterns were also observed in the 2DE gel of different extraction methods used for each type of sample.

  2. The impact of freeze-drying infant fecal samples on measures of their bacterial community profiles and milk-derived oligosaccharide content.

    Science.gov (United States)

    Lewis, Zachery T; Davis, Jasmine C C; Smilowitz, Jennifer T; German, J Bruce; Lebrilla, Carlito B; Mills, David A

    2016-01-01

    Infant fecal samples are commonly studied to investigate the impacts of breastfeeding on the development of the microbiota and subsequent health effects. Comparisons of infants living in different geographic regions and environmental contexts are needed to aid our understanding of evolutionarily-selected milk adaptations. However, the preservation of fecal samples from individuals in remote locales until they can be processed can be a challenge. Freeze-drying (lyophilization) offers a cost-effective way to preserve some biological samples for transport and analysis at a later date. Currently, it is unknown what, if any, biases are introduced into various analyses by the freeze-drying process. Here, we investigated how freeze-drying affected analysis of two relevant and intertwined aspects of infant fecal samples, marker gene amplicon sequencing of the bacterial community and the fecal oligosaccharide profile (undigested human milk oligosaccharides). No differences were discovered between the fecal oligosaccharide profiles of wet and freeze-dried samples. The marker gene sequencing data showed an increase in proportional representation of Bacteriodes and a decrease in detection of bifidobacteria and members of class Bacilli after freeze-drying. This sample treatment bias may possibly be related to the cell morphology of these different taxa (Gram status). However, these effects did not overwhelm the natural variation among individuals, as the community data still strongly grouped by subject and not by freeze-drying status. We also found that compensating for sample concentration during freeze-drying, while not necessary, was also not detrimental. Freeze-drying may therefore be an acceptable method of sample preservation and mass reduction for some studies of microbial ecology and milk glycan analysis.

  3. The impact of freeze-drying infant fecal samples on measures of their bacterial community profiles and milk-derived oligosaccharide content

    Directory of Open Access Journals (Sweden)

    Zachery T. Lewis

    2016-01-01

    Full Text Available Infant fecal samples are commonly studied to investigate the impacts of breastfeeding on the development of the microbiota and subsequent health effects. Comparisons of infants living in different geographic regions and environmental contexts are needed to aid our understanding of evolutionarily-selected milk adaptations. However, the preservation of fecal samples from individuals in remote locales until they can be processed can be a challenge. Freeze-drying (lyophilization offers a cost-effective way to preserve some biological samples for transport and analysis at a later date. Currently, it is unknown what, if any, biases are introduced into various analyses by the freeze-drying process. Here, we investigated how freeze-drying affected analysis of two relevant and intertwined aspects of infant fecal samples, marker gene amplicon sequencing of the bacterial community and the fecal oligosaccharide profile (undigested human milk oligosaccharides. No differences were discovered between the fecal oligosaccharide profiles of wet and freeze-dried samples. The marker gene sequencing data showed an increase in proportional representation of Bacteriodes and a decrease in detection of bifidobacteria and members of class Bacilli after freeze-drying. This sample treatment bias may possibly be related to the cell morphology of these different taxa (Gram status. However, these effects did not overwhelm the natural variation among individuals, as the community data still strongly grouped by subject and not by freeze-drying status. We also found that compensating for sample concentration during freeze-drying, while not necessary, was also not detrimental. Freeze-drying may therefore be an acceptable method of sample preservation and mass reduction for some studies of microbial ecology and milk glycan analysis.

  4. Inorganic profile of some Brazilian medicinal plants obtained from ethanolic extract and ''in natura'' samples

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, M.O.M.; de Sousa, P.T.; Salvador, V.L.R.; Sato, I.M.

    2004-10-03

    The Anadenathera macrocarpa, Schinus molle, Hymenaea courbaril, Cariniana legalis, Solidago microglossa and Stryphnodendron barbatiman, were collected ''in natura'' samples (leaves, flowers, barks and seeds) from different commercial suppliers. The pharmaco-active compounds in ethanolic extracts had been made by the Mato Grosso Federal University (UFMT). The energy-dispersive x-ray fluorescence (ED-XRF) spectrometry was used for the elemental analysis in different parts of the plants and respective ethanolic extracts. The Ca, Cl, Cu, Fe, K, Mg, Mn, Na, Ni, P, Rb, S, Sr and Zn concentrations were determined by the fundamental parameters method. Some specimens showed a similar inorganic profile for ''in natura'' and ethanolic extract samples and some ones showed a distinct inorganic profile. For example, the Anadenathera macrocarpa showed a similar concentration in Mg, P, Cu, Zn and Rb elements in ''in natura'' and ethanolic extract samples; however very different concentration in Na, S, Cl, K , Ca, Mn, Fe and Sr was observed in distinctive samples. The Solidago microglossa showed the K, Ca, Cl, S, Mg, P and Fe elements as major constituents in both samples, suggesting that the extraction process did not affect in a considerable way the ''in natura'' inorganic composition. The elemental composition of the different parts of the plants (leaves, flowers, barks and seeds) has been also determined. For example, the Schinus molle specimen showed P, K, Cl and Ca elements as major constituents in the seeds, Mg, K and Sr in the barks and Mg, S, Cl and Mn in the leaves, demonstrating a differentiated elementary distribution. These inorganic profiles will contribute to evaluate the quality control of the Brazilian herbaceous trade and also will assist to identify which parts of the medicinal plants has greater therapeutic effect.

  5. A novel putative lipoprotein receptor (CasLpR) in the hemocytes of the blue crab, Callinectes sapidus: cloning and up-regulated expression after the injection of LPS and LTA.

    Science.gov (United States)

    Tsutsui, Naoaki; Chung, J Sook

    2012-03-01

    The full-length cDNA encoding a putative lipoprotein receptor (CasLpR) was isolated from the hemocytes of Callinectes sapidus using 5' and 3' RACEs. The open reading frame for CasLpR contains a precursor of putative CasLpR consisting of 1710 amino acid residues including 22 amino acid residues of the signal peptide (22 amino acids). Mature CasLpR (1688 amino acids with 5.6% of phosphorylation sites) has multiple, putative functional domains: five low-density lipoprotein receptor domains in the N-terminus, and a G-protein-coupled receptor proteolysis site domain and a 7 transmembrane receptor (secretin family) domain in the C-terminus. To date, there are no proteins with a similar domain structure in the GenBank. The expression pattern of CasLpR was exclusive in hemocytes among all tested tissues obtained from a juvenile female at intermolt stage: brain, eyestalk ganglia, pericardial organs, and thoracic ganglia complex (nervous system); hepatopancreas (digestive system); heart, artery and hemocytes (circulatory system); gill and antennal gland (excretory system), hypodermis; and Y-organ (endocrine organ). There was no CasLpR expression in the ovary of an adult female. A putative function of CasLpR was examined after challenges of lipopolysaccharides (LPS) and lipoteichoic acid (LTA) in vivo using qRT-PCR assays. Animals at 24 h after injection of LPS or LTA up-regulated the expression of CasLpR in hemocytes by ∼3.5 and 1.4 folds, respectively, compared to the controls that received saline injection. LPS challenge also caused the greatest increment (∼55 folds) of heat shock protein 90 (Hsp90) expression in these samples. These data indicate that putative CasLpR and CasHsp90 may be involved in the defense system or the stress response of C. sapidus. Copyright © 2011 Elsevier Ltd. All rights reserved.

  6. Evaluation of volatile profiles obtained for minimally-processed pineapple fruit samples during storage by headspace-solid phase microextraction gas chromatography-mass spectrometry

    Directory of Open Access Journals (Sweden)

    Francielle Crocetta TURAZZI

    Full Text Available Abstract This paper describes the application of the solid-phase microextraction (SPME technique for the determination and monitoring of the volatile profile of minimally-processed pineapple fruit stored at various temperatures (-12 °C, 4 °C and 25 °C for different periods (1, 4 and 10 days. The SPME fiber coating composed of Car/PDMS presented the best performance. The optimal extraction conditions obtained through a Doehlert design were 60 min at 35 °C. The profiles for the volatile compounds content of the fruit at each stage of storage were determined by gas chromatography-mass spectrometry (GC-MS. The variation in the volatile profile over time was greater when the fruit samples were stored at 25 °C and at -12 °C compared to 4 °C. Thus, according to the volatile profiles associated with the storage conditions evaluated in this study, packaged pineapple retains best its fresh fruit aroma when stored at 4 °C.

  7. Selective 5-HT7 receptor agonists LP 44 and LP 211 elicit an analgesic effect on formalin-induced orofacial pain in mice

    Science.gov (United States)

    DEMİRKAYA, Kadriye; AKGÜN, Özlem Martı; ŞENEL, Buğra; ÖNCEL TORUN, Zeynep; SEYREK, Melik; LACİVİTA, Enza; LEOPOLDO, Marcello; DOĞRUL, Ahmet

    2016-01-01

    ABSTRACT The most recently identified serotonin (5-HT) receptor is the 5-HT7 receptor. The antinociceptive effects of a 5-HT7 receptor agonist have been shown in neuropathic and inflammatory animal models of pain. A recent study demonstrated the functional expression of 5-HT7 receptors in the substantia gelatinosa (SG) of the trigeminal subnucleus caudalis, which receives and processes orofacial nociceptive inputs. Objective To investigate the antinociceptive effects of pharmacological activation of 5-HT7 receptors on orofacial pain in mice. Material and Methods Nociception was evaluated by using an orofacial formalin test in male Balb-C mice. Selective 5-HT7 receptor agonists, LP 44 and LP 211 (1, 5, and 10 mg/kg), were given intraperitoneally 30 min prior to a formalin injection. A bolus of 10 µl of 4% subcutaneous formalin was injected into the upper lip of mice and facial grooming behaviors were monitored. The behavioral responses consisted of two distinct periods, the early phase corresponding to acute pain (Phase I: 0–12 min) and the late phase (Phase II: 12–30 min). Results LP 44 and LP 211 (1, 5, and 10 mg/kg) produced an analgesic effect with reductions in face rubbing time in both Phase I and Phase II of the formalin test. Conclusion Our results suggest that 5-HT7 receptor agonists may be promising analgesic drugs in the treatment of orofacial pain. PMID:27383702

  8. Selective 5-HT7 receptor agonists LP 44 and LP 211 elicit an analgesic effect on formalin-induced orofacial pain in mice.

    Science.gov (United States)

    Demirkaya, Kadriye; Akgün, Özlem Martı; Şenel, Buğra; Öncel Torun, Zeynep; Seyrek, Melik; Lacivita, Enza; Leopoldo, Marcello; Doğrul, Ahmet

    2016-01-01

    To investigate the antinociceptive effects of pharmacological activation of 5-HT7 receptors on orofacial pain in mice. Nociception was evaluated by using an orofacial formalin test in male Balb-C mice. Selective 5-HT7 receptor agonists, LP 44 and LP 211 (1, 5, and 10 mg/kg), were given intraperitoneally 30 min prior to a formalin injection. A bolus of 10 µl of 4% subcutaneous formalin was injected into the upper lip of mice and facial grooming behaviors were monitored. The behavioral responses consisted of two distinct periods, the early phase corresponding to acute pain (Phase I: 0-12 min) and the late phase (Phase II: 12-30 min). LP 44 and LP 211 (1, 5, and 10 mg/kg) produced an analgesic effect with reductions in face rubbing time in both Phase I and Phase II of the formalin test. Our results suggest that 5-HT7 receptor agonists may be promising analgesic drugs in the treatment of orofacial pain.

  9. Equalizer Complexity for 6-LP Mode 112 Gbit/s m-ary DP-QAM Space Division Multiplexed Transmission in Strongly Coupled Few-Mode-Fibers

    DEFF Research Database (Denmark)

    Asif, Rameez; Ye, Feihong; Morioka, Toshio

    2015-01-01

    We have quantified equalizer complexity for transmitting dual-polarized 6-LP modes (LP01, LP11a, LP11b, LP21a, LP21b and LP02) of 112 Gbit/s m-ary QAM (m=4, 16, 32, 64, 256) single carrier signals over 20 km step-index few-mode fiber. The transmitted signals are strongly coupled and recovered using...... 12×12 multiple-input multiple-output (MIMO) adaptive digital signal processing (DSP). Time domain equalization (TDE) is realized to implement the linear adaptive MIMO module that is implemented in blind mode using conventional constant-modulusalgorithm (CMA) for finite impulse response (FIR) adaptive...

  10. Chemical profile and anti-leishmanial activity of three Ecuadorian propolis samples from Quito, Guayaquil and Cotacachi regions.

    Science.gov (United States)

    Cuesta-Rubio, Osmany; Campo Fernández, Mercedes; Márquez Hernández, Ingrid; Jaramillo, Carmita Gladys Jaramillo; González, Victor Hugo; Montes De Oca Porto, Rodny; Marrero Delange, David; Monzote Fidalgo, Lianet; Piccinelli, Anna Lisa; Campone, Luca; Rastrelli, Luca

    2017-07-01

    Three propolis samples were collected from different regions of Ecuador (Quito, Guayaquil and Cotacachi) and their methanolic extracts were prepared. Preliminary information supplied by TLC and NMR data, allowed us to define two main types of propolis: Cotacachi propoli sample (CPS), rich in flavonoids and Quito and Guayaquil samples (QPS and GPS) containing triterpenic alcohols and acetyl triterpenes as the main constituents. Two different approaches based on RP-HPLC preparative procedure and NMR structural determination (CPS) and GC-MS analysis (QPS and GPS) were successfully used for the chemical characterization of their major compounds. All three propolis extracts were able to inhibit Leishmania amazonensis growth but propolis sample rich in flavonoids was the most active (IC50=17.1±1.7μg/mL). In the literature this is the first study on propolis from Ecuador. Copyright © 2017. Published by Elsevier B.V.

  11. Extensive surface protein profiles of extracellular vesicles from cancer cells may provide diagnostic signatures from blood samples

    Directory of Open Access Journals (Sweden)

    Larissa Belov

    2016-04-01

    Full Text Available Extracellular vesicles (EV are membranous particles (30–1,000 nm in diameter secreted by cells. Important biological functions have been attributed to 2 subsets of EV, the exosomes (bud from endosomal membranes and the microvesicles (MV; bud from plasma membranes. Since both types of particles contain surface proteins derived from their cell of origin, their detection in blood may enable diagnosis and prognosis of disease. We have used an antibody microarray (DotScan to compare the surface protein profiles of live cancer cells with those of their EV, based on their binding patterns to immobilized antibodies. Initially, EV derived from the cancer cell lines, LIM1215 (colorectal cancer and MEC1 (B-cell chronic lymphocytic leukaemia; CLL, were used for assay optimization. Biotinylated antibodies specific for EpCAM (CD326 and CD19, respectively, were used to detect captured particles by enhanced chemiluminescence. Subsequently, this approach was used to profile CD19+ EV from the plasma of CLL patients. These EV expressed a subset (~40% of the proteins detected on CLL cells from the same patients: moderate or high levels of CD5, CD19, CD31, CD44, CD55, CD62L, CD82, HLA-A,B,C, HLA-DR; low levels of CD21, CD49c, CD63. None of these proteins was detected on EV from the plasma of age- and gender-matched healthy individuals.

  12. Sample size estimation to substantiate freedom from disease for clustered binary data with a specific risk profile

    DEFF Research Database (Denmark)

    Kostoulas, P.; Nielsen, Søren Saxmose; Browne, W. J.

    2013-01-01

    SUMMARY Disease cases are often clustered within herds or generally groups that share common characteristics. Sample size formulae must adjust for the within-cluster correlation of the primary sampling units. Traditionally, the intra-cluster correlation coefficient (ICC), which is an average meas...... subsp. paratuberculosis infection, in Danish dairy cattle and a study on critical control points for Salmonella cross-contamination of pork, in Greek slaughterhouses....

  13. [Genetic profiling of Giardia intestinalis by polimerase chain in human and dogs samples of Colombian Caribean Coast].

    Science.gov (United States)

    Arroyo-Salgado, Bárbara; Buelvas-Montes, Yaleyvis; Villalba-Vizcaíno, Vivian; Salomón-Arzuza, Octavio

    2014-01-01

    Giardia intestinalis (G. Intestinalis) is a protozoan that causes diarrheal disease and malabsorption syndrome in humans and other mammals. It presents a high genetic diversity evidenced in the recognition of 7 genotypes (A-G). Genotypes A and B are commonly associated to humans and domestic animals such as dogs. The aim of this study was to conduct a preliminary genetic characterization of G. intestinalis in humans and dogs from two cities on the Caribbean coast of Colombia. Sampling areas were selected according to the highest numbers of acute diarrheal disease. Stool samples were collected from children under 7 years old, with positive medical tests for G. intestinalis. Cysts were purified by sucrose gradient and DNA samples were isolated by extraction with organic solvents. Molecular characterization was performed by amplifying the gene triose phosphate isomerase (tpi) by using a semi-nested PCR. A total of 202 samples of DNA were obtained; of these, 111 were positive in coproparasitological analysis (13 dogs and 98 children). Genotype distribution in positive samples was: 5.1% belonged to genotype A and 92.3% to genotype B. Genotype B was present in humans and animals. The most common genotype in both human and animal samples was genotype B, suggesting a zoonotic transmission cycle. Copyright © 2012 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

  14. Characterization of Lactobacillus plantarum Lp27 isolated from Tibetan kefir grains: a potential probiotic bacterium with cholesterol-lowering effects.

    Science.gov (United States)

    Huang, Ying; Wu, Fei; Wang, Xiaojun; Sui, Yujie; Yang, Longfei; Wang, Jinfeng

    2013-05-01

    Lactobacillus plantarum Lp27 was isolated from Tibetan kefir grains. The Lp27 isolate survived a 3-h incubation at pH 2.0 and grew normally in 0.3% oxgall. In addition, the Lp27 isolate exhibited an adhesion ratio of 9.5 ± 2.5% with Caco-2 cells. Antibiotic susceptibility tests indicated that the Lp27 isolate was sensitive to gentamicin, tetracycline, erythromycin, and chloramphenicol, and was resistant to vancomycin with a minimum inhibitory value of 23µg/mL. The Lp27 isolate inhibited cholesterol absorption through downregulation of Niemann-Pick C1-like 1 (NPC1L1) expression in Caco-2 cells. The Lp27 isolate was fed to hypercholesterolemic rats at a dose of 10(9) cfu/d for 4wk. The Lp27 feeding significantly lowered serum total cholesterol, low-density lipoprotein cholesterol, and triglycerides concentrations, but no change was observed in the serum high-density lipoprotein cholesterol concentrations. In addition, liver total cholesterol and triglycerides were decreased in the Lp27-fed group. The expression of NPC1L1 in the duodenum and jejunum was significantly decreased following Lp27 feeding. These results indicate that Lp27 might be an effective cholesterol-lowering probiotic and a possible mechanism for the cholesterol-reducing effects of probiotics. Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  15. Expression profiling of blood samples from an SU5416 Phase III metastatic colorectal cancer clinical trial: a novel strategy for biomarker identification

    Directory of Open Access Journals (Sweden)

    Smolich Beverly D

    2003-02-01

    Full Text Available Abstract Background Microarray-based gene expression profiling is a powerful approach for the identification of molecular biomarkers of disease, particularly in human cancers. Utility of this approach to measure responses to therapy is less well established, in part due to challenges in obtaining serial biopsies. Identification of suitable surrogate tissues will help minimize limitations imposed by those challenges. This study describes an approach used to identify gene expression changes that might serve as surrogate biomarkers of drug activity. Methods Expression profiling using microarrays was applied to peripheral blood mononuclear cell (PBMC samples obtained from patients with advanced colorectal cancer participating in a Phase III clinical trial. The PBMC samples were harvested pre-treatment and at the end of the first 6-week cycle from patients receiving standard of care chemotherapy or standard of care plus SU5416, a vascular endothelial growth factor (VEGF receptor tyrosine kinase (RTK inhibitor. Results from matched pairs of PBMC samples from 23 patients were queried for expression changes that consistently correlated with SU5416 administration. Results Thirteen transcripts met this selection criterion; six were further tested by quantitative RT-PCR analysis of 62 additional samples from this trial and a second SU5416 Phase III trial of similar design. This method confirmed four of these transcripts (CD24, lactoferrin, lipocalin 2, and MMP-9 as potential biomarkers of drug treatment. Discriminant analysis showed that expression profiles of these 4 transcripts could be used to classify patients by treatment arm in a predictive fashion. Conclusions These results establish a foundation for the further exploration of peripheral blood cells as a surrogate system for biomarker analyses in clinical oncology studies.

  16. Not-so-clean fun: a profile of bath salt users among a college sample in the United States.

    Science.gov (United States)

    Miller, Bryan Lee; Stogner, John M

    2014-01-01

    This research examines the characteristics of users of synthetic stimulants marketed as "bath salts." Synthetic stimulants such as MDPV (3,4-Methylenedioxypyrovalerone), Mephedrone (4-Methylmethcathinone), and Methylone (3,4-Methylenedioxymethcathinone) are often contained in products sold at convenience stores and over the Internet in the United States. Despite the recent legal action banning these types of synthetic stimulants, little is known about the characteristics of the users of these substances. This research provides a profile of bath salt users in the United States among an emerging adult population. A self-report survey instrument was administered to 2,349 students at a large university in the southeastern United States. Respondents indicated whether they had used synthetic stimulants and reported demographic characteristics. Results indicated that users of bath salts were more likely to be male, Hispanic or Native American, student athletes, employed, identify as a members of the LGBT community, and users of other substances.

  17. HUBUNGAN PENGGUNAAN LP-LPO DENGAN KECUKUPAN DAN KERASIONALAN PENGGUNAAN OBAT DI PUSKESMAS

    Directory of Open Access Journals (Sweden)

    Max Joseph Herman

    2012-09-01

    Full Text Available A study to determine the impact of the implementation of LP-LPO (Drug-use Report and Drug-request Form upon the availability and the rational use of drugs at Puskesmas (Health Centre had been carried out during 1998. The study location were four districts, i.e. Pasaman and Agam in West Sumatera and Pasuruan and Malang in East Java. The survey was conducted retrospectively by visiting 40 PHCs selected purposively to interview, observe and tranfer data from 1997/1998 LP-LPO. Data concerning the implementation of LP-LPO through some variables, the availability of drugs and an evaluation of rational prescription based on the standard treatment guidelines in the year of 1997/1998 were collected. Multiple regression was used to analyze the correlation among variables of the implementation of LP-LPO and the availability as well as the rational use of drugs, following a general description of each variable involved at selected HCs. The study revealed good results in the implementation of LP-LPO to manage the availability of indicator drugs at HCs. However, at most HCs the drug supply did not fit the request. The percentages of HCs in implementing LP-LPO in terms of variables of planning, scheduling, record keeping, timing of reporting, methods and data resources ranged from 50 to 80%. Statistically there was a significant correlation between the implementation of LP-LPO in terms of the above variables and the availability of drugs. On the contrary, there was no statistically significant correlation between drug availability and rational prescription, which was found only at 5% of HCs.

  18. SaVanT: a web-based tool for the sample-level visualization of molecular signatures in gene expression profiles.

    Science.gov (United States)

    Lopez, David; Montoya, Dennis; Ambrose, Michael; Lam, Larry; Briscoe, Leah; Adams, Claire; Modlin, Robert L; Pellegrini, Matteo

    2017-10-25

    Molecular signatures are collections of genes characteristic of a particular cell type, tissue, disease, or perturbation. Signatures can also be used to interpret expression profiles generated from heterogeneous samples. Large collections of gene signatures have been previously developed and catalogued in the MSigDB database. In addition, several consortia and large-scale projects have systematically profiled broad collections of purified primary cells, molecular perturbations of cell types, and tissues from specific diseases, and the specificity and breadth of these datasets can be leveraged to create additional molecular signatures. However, to date there are few tools that allow the visualization of individual signatures across large numbers of expression profiles. Signature visualization of individual samples allows, for example, the identification of patient subcategories a priori on the basis of well-defined molecular signatures. Here, we generate and compile 10,985 signatures (636 newly-generated and 10,349 previously available from MSigDB) and provide a web-based Signature Visualization Tool (SaVanT; http://newpathways.mcdb.ucla.edu/savant ), to visualize these signatures in user-generated expression data. We show that using SaVanT, immune activation signatures can distinguish patients with different types of acute infections (influenza A and bacterial pneumonia). Furthermore, SaVanT is able to identify the prominent signatures within each patient group, and identify the primary cell types underlying different leukemias (acute myeloid and acute lymphoblastic) and skin disorders. The development of SaVanT facilitates large-scale analysis of gene expression profiles on a patient-level basis to identify patient subphenotypes, or potential therapeutic target pathways.

  19. Meta-analysis of gene expression profiles associated with histological classification and survival in 829 ovarian cancer samples.

    Science.gov (United States)

    Fekete, Tibor; Rásó, Erzsébet; Pete, Imre; Tegze, Bálint; Liko, István; Munkácsy, Gyöngyi; Sipos, Norbert; Rigó, János; Györffy, Balázs

    2012-07-01

    Transcriptomic analysis of global gene expression in ovarian carcinoma can identify dysregulated genes capable to serve as molecular markers for histology subtypes and survival. The aim of our study was to validate previous candidate signatures in an independent setting and to identify single genes capable to serve as biomarkers for ovarian cancer progression. As several datasets are available in the GEO today, we were able to perform a true meta-analysis. First, 829 samples (11 datasets) were downloaded, and the predictive power of 16 previously published gene sets was assessed. Of these, eight were capable to discriminate histology subtypes, and none was capable to predict survival. To overcome the differences in previous studies, we used the 829 samples to identify new predictors. Then, we collected 64 ovarian cancer samples (median relapse-free survival 24.5 months) and performed TaqMan Real Time Polimerase Chain Reaction (RT-PCR) analysis for the best 40 genes associated with histology subtypes and survival. Over 90% of subtype-associated genes were confirmed. Overall survival was effectively predicted by hormone receptors (PGR and ESR2) and by TSPAN8. Relapse-free survival was predicted by MAPT and SNCG. In summary, we successfully validated several gene sets in a meta-analysis in large datasets of ovarian samples. Additionally, several individual genes identified were validated in a clinical cohort. Copyright © 2011 UICC.

  20. PARTICULATE ORGANIC CARBON and PARTICULATE ORGANIC NITROGEN profile, discrete sample and site samples data collected in the South Atlantic Ocean and South Pacific Ocean on the NATHANIEL B. PALMER cruises NBP0103, NBP0104 and others as part of the Southern Ocean GLOBEC project from 2001-04-29 to 2002-09-12 (NODC Accession 0112164)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112164 includes profile, discrete sample, site samples and chemical data collected aboard the NATHANIEL B. PALMER during cruises NBP0103, NBP0104,...

  1. Cancer associated epigenetic transitions identified by genome-wide histone methylation binding profiles in human colorectal cancer samples and paired normal mucosa

    Directory of Open Access Journals (Sweden)

    Wallerman Ola

    2011-10-01

    Full Text Available Abstract Background Despite their well-established functional roles, histone modifications have received less attention than DNA methylation in the cancer field. In order to evaluate their importance in colorectal cancer (CRC, we generated the first genome-wide histone modification profiles in paired normal colon mucosa and tumor samples. Methods Chromatin immunoprecipitation and microarray hybridization (ChIP-chip was used to identify promoters enriched for histone H3 trimethylated on lysine 4 (H3K4me3 and lysine 27 (H3K27me3 in paired normal colon mucosa and tumor samples from two CRC patients and for the CRC cell line HT29. Results By comparing histone modification patterns in normal mucosa and tumors, we found that alterations predicted to have major functional consequences were quite rare. Furthermore, when normal or tumor tissue samples were compared to HT29, high similarities were observed for H3K4me3. However, the differences found for H3K27me3, which is important in determining cellular identity, indicates that cell lines do not represent optimal tissue models. Finally, using public expression data, we uncovered previously unknown changes in CRC expression patterns. Genes positive for H3K4me3 in normal and/or tumor samples, which are typically already active in normal mucosa, became hyperactivated in tumors, while genes with H3K27me3 in normal and/or tumor samples and which are expressed at low levels in normal mucosa, became hypersilenced in tumors. Conclusions Genome wide histone modification profiles can be used to find epigenetic aberrations in genes associated with cancer. This strategy gives further insights into the epigenetic contribution to the oncogenic process and may identify new biomarkers.

  2. Physicochemical characteristics and sensory profile of honey samples from stingless bees (Apidae: Meliponinae) submitted to a dehumidification process.

    Science.gov (United States)

    Carvalho, Carlos A L; Sodré, Geni S; Fonseca, Antonio A O; Alves, Rogério M O; Souza, Bruno A; Clarton, Lana

    2009-03-01

    This study was conducted to evaluate the effect of a dehumidification process on the physicochemical and sensory characteristics of stingless-bee honey. Melipona scutellaris and M. quadrifasciata honey samples were submitted to a dehumidification process and to physicochemical (reducing sugars, apparent sucrose, moisture, diastatic activity, hydroxymethylfurfural, ash, pH, acidity, and electric conductivity) and sensory evaluations (fluidity, color, aroma, crystallization,flavor,and acceptability). The results indicated that the dehumidification process does not interfere with honey quality and acceptability.

  3. Metabolomics profiling of pre-and post-anesthesia plasma samples of colorectal patients obtained via Ficoll separation

    OpenAIRE

    Ghini, Veronica; Unger, Florian T; Tenori, Leonardo; Turano, Paola; Juhl, Hartmut; David, Kerstin A.

    2015-01-01

    1H NMR spectroscopy was used to investigate the metabolic consequences of general anesthesia in the plasma of two groups of patients with diagnosis for non-metastatic colorectal cancer and metastatic colorectal cancer with liver-metastasis, respectively. Patients were treated with etomidate or propofol, two frequently used sedation agents. Plasma samples were obtained via Ficoll separation. Here, we demonstrated that this procedure introduces a number of limitations for NMR-based metabolomics...

  4. Diagnostic Role of MicroRNA Expression Profile in the Prenatal Amniotic Fluid Samples of Pregnant Women with Down Syndrome.

    Science.gov (United States)

    Karaca, Emin; Aykut, Ayça; Ertürk, Biray; Durmaz, Burak; Güler, Ahmet; Büke, Barış; Yeniel, Ahmet Özgür; Ergenoğlu, Ahmet Mete; Özkınay, Ferda; Özeren, Mehmet; Kazandı, Mert; Akercan, Fuat; Sağol, Sermet; Gündüz, Cumhur; Çoğulu, Özgür

    2017-12-08

    Down syndrome is the most common chromosomal anomaly in humans. Down syndrome is the most common chromosomal anomaly in humans affecting people from every race and age. Most of the cases are can be diagnosed by prenatal diagnostic methods in pregnancy. Due to the longtime of culture method applied for prenatal diagnosis, genetic analysis has been developed and developing for rapid diagnosis. For this reason, the effective use of miRNAs was investigated in the rapid analysis of prenatal samples with Down syndrome. We evaluated the expression levels of 14 miRNAs, located on chromosome 21 in prenatal samples and their utility for prenatal testing of Down syndrome. Case-control study. A total of 56 patients who underwent invasive prenatal testing, 23 carrying fetuses with DS; 33 control cases carrying fetuses with normal karyotype, were included. Indications for invasive prenatal testing were advanced maternal age and increased risk of Down syndrome in screening tests. Gestation weeks ranged between 17-18 in study and control groups. MicroRNA expression levels were measured by real-time-polymerase chain reaction. miR-125b-2, miR-155 and miR-3156 expression levels were significantly higher in prenatal samples. Significantly dysregulated miRNAs either may be associated with the phenotype or may be the result of abnormal development. Further large scale comparative studies carried out in a variety of conditions may bring novel insights in the field of abnormal conditions.

  5. Analysis of error floor of LDPC codes under LP decoding over the BSC

    Energy Technology Data Exchange (ETDEWEB)

    Chertkov, Michael [Los Alamos National Laboratory; Chilappagari, Shashi [UNIV OF AZ; Vasic, Bane [UNIV OF AZ; Stepanov, Mikhail [UNIV OF AZ

    2009-01-01

    We consider linear programming (LP) decoding of a fixed low-density parity-check (LDPC) code over the binary symmetric channel (BSC). The LP decoder fails when it outputs a pseudo-codeword which is not a codeword. We propose an efficient algorithm termed the instanton search algorithm (ISA) which, given a random input, generates a set of flips called the BSC-instanton and prove that: (a) the LP decoder fails for any set of flips with support vector including an instanton; (b) for any input, the algorithm outputs an instanton in the number of steps upper-bounded by twice the number of flips in the input. We obtain the number of unique instantons of different sizes by running the ISA sufficient number of times. We then use the instanton statistics to predict the performance of the LP decoding over the BSC in the error floor region. We also propose an efficient semi-analytical method to predict the performance of LP decoding over a large range of transition probabilities of the BSC.

  6. Genetic invalidation of Lp-PLA2 as a therapeutic target

    DEFF Research Database (Denmark)

    Gregson, John M; Freitag, Daniel F; Surendran, Praveen

    2017-01-01

    AIMS: Darapladib, a potent inhibitor of lipoprotein-associated phospholipase A2 (Lp-PLA2), has not reduced risk of cardiovascular disease outcomes in recent randomized trials. We aimed to test whether Lp-PLA2 enzyme activity is causally relevant to coronary heart disease. METHODS: In 72......,657 patients with coronary heart disease and 110,218 controls in 23 epidemiological studies, we genotyped five functional variants: four rare loss-of-function mutations (c.109+2T > C (rs142974898), Arg82His (rs144983904), Val279Phe (rs76863441), Gln287Ter (rs140020965)) and one common modest-impact variant...... to compare effects of darapladib treatment on soluble Lp-PLA2 activity, conventional cardiovascular risk factors, and coronary heart disease risk with corresponding effects of Lp-PLA2-lowering alleles. RESULTS: Lp-PLA2 activity was decreased by 64% (p = 2.4 × 10(-25)) with carriage of any of the four loss...

  7. [Changes in lipoprotein (a) [Lp(a)] level after an ischemic stroke].

    Science.gov (United States)

    Wehr, Hanna; Rado, Maria; Mendel, Tadeusz; Swiderska, Małgorzata; Bednarska-Makaruk, Małgorzata; Szirkowiec, Walentyna

    2004-01-01

    The aim of the work was to recognize whether often observed high levels of apolipoprotein (a) [Lp(a)] in patients shortly after an ischemic stroke are a result of the acute phase reaction. In 13 patients Lp(a) was determined within the first 24 hours after the stroke onset, after the next 7 days and after three months i.e. when it could be considered that Lp(a) level was the same as before onset of the disease. In 17 patients only two determinations were performed. Another acute phase indicator: C-reactive protein (CRP), as well as serum lipids were also determined. CRP level was increased in the first determination and increased further after 7 days. After three months it returned to low values. High density lipoprotein (HDL) cholesterol which demonstrates a negative acute phase response changed in the opposite way. No similar fluctuations of Lp(a) level were observed. It can be concluded that during the investigated period Lp(a) had no properties of the acute phase reactant.

  8. MicroRNA profiling of dogs with transitional cell carcinoma of the bladder using blood and urine samples.

    Science.gov (United States)

    Kent, Michael S; Zwingenberger, Allison; Westropp, Jodi L; Barrett, Laura E; Durbin-Johnson, Blythe P; Ghosh, Paramita; Vinall, Ruth L

    2017-11-15

    Early signs of canine transitional cell carcinoma (TCC) are frequently assumed to be caused by other lower urinary tract diseases (LUTD) such as urinary tract infections, resulting in late diagnosis of TCC which could be fatal. The development of a non-invasive clinical test for TCC could dramatically reduce mortality. To determine whether microRNAs (miRNAs) can be used as non-invasive diagnostic biomarkers, we assessed miRNA expression in blood and/or urine from dogs with clinically normal bladders (n = 28), LUTD (n = 25), and TCC (n = 17). Expression levels of 5 miRNA associated with TCC pathophysiology (miR-34a, let-7c, miR-16, miR-103b, and miR-106b) were assessed by quantitative real-time PCR. Statistical analyses using ranked ANOVA identified significant differences in miR-103b and miR-16 levels between urine samples from LUTD and TCC patients (miR-103b, p = 0.002; and miR-16, p = 0.016). No statistically significant differences in miRNA levels were observed between blood samples from LUTD versus TCC patients. Expression levels of miR-34a trended with miR-16, let-7c, and miR-103b levels in individual normal urine samples, however, this coordination was completely lost in TCC urine samples. In contrast, co-ordination of miR-34a, miR-16, let-7c, and miR-103b expression levels was maintained in blood samples from TCC patients. Our combined data indicate a potential role for miR-103b and miR-16 as diagnostic urine biomarkers for TCC, and that further investigation of miR-103b and miR-16 in the dysregulation of coordinated miRNA expression in bladder carcinogenesis is warranted.

  9. Cross-reactivity profiles of hybrid capture II, cobas, and APTIMA human papillomavirus assays: split-sample study.

    Science.gov (United States)

    Preisler, Sarah; Rebolj, Matejka; Ejegod, Ditte Møller; Lynge, Elsebeth; Rygaard, Carsten; Bonde, Jesper

    2016-07-20

    High-risk Human Papillomavirus (HPV) testing is replacing cytology in cervical cancer screening as it is more sensitive for preinvasive cervical lesions. However, the bottleneck of HPV testing is the many false positive test results (positive tests without cervical lesions). Here, we evaluated to what extent these can be explained by cross-reactivity, i.e. positive test results without evidence of high-risk HPV genotypes. The patterns of cross-reactivity have been thoroughly studied for hybrid capture II (HC2) but not yet for newer HPV assays although the manufacturers claimed no or limited frequency of cross-reactivity. In this independent study we evaluated the frequency of cross-reactivity for HC2, cobas, and APTIMA assays. Consecutive routine cervical screening samples from 5022 Danish women, including 2859 from women attending primary screening, were tested with the three evaluated DNA and mRNA HPV assays. Genotyping was undertaken using CLART HPV2 assay, individually detecting 35 genotypes. The presence or absence of cervical lesions was determined with histological examinations; women with abnormal cytology were managed as per routine recommendations; those with normal cytology and positive high-risk HPV test results were invited for repeated testing in 18 months. Cross-reactivity to low-risk genotypes was detected in 109 (2.2 %) out of 5022 samples on HC2, 62 (1.2 %) on cobas, and 35 (0.7 %) on APTIMA with only 10 of the samples cross-reacting on all 3 assays. None of the 35 genotypes was detected in 49 (1.0 %), 162 (3.2 %), and 56 (1.1 %) samples, respectively. In primary screening at age 30 to 65 years (n = 2859), samples of 72 (25 %) out of 289 with high-risk infections on HC2 and < CIN2 histology were due to cross-reactivity. On cobas, this was 106 (26 %) out of 415, and on APTIMA 48 (21 %) out of 224. Despite manufacturer claims, all three assays showed cross-reactivity. In primary cervical screening at age ≥30 years, cross

  10. The lysine-peptoid hybrid LP5 maintain activity under physiological conditions and affects virulence gene expression in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Gottschalk, Sanne; Ingmer, Hanne; Thomsen, Line E.

    2016-01-01

    The antimicrobial peptide, LP5, is a lysine-peptoid hybrid, with antimicrobial activity against clinically relevant bacteria. Here, we investigated how various environmental conditions affect the antimicrobial activity of LP5 against Staphylococcus aureus (S. aureus). We found that LP5 maintained...... to sub-inhibitory concentrations of LP5 affected the expression of the major virulence factors of S. aureus, revealing a potential as anti virulence compound. Thus, these results show how environmental factors affect the peptide efficiency and further add to the knowledge on how the peptide affects S...

  11. Circulating and PBMC Lp-PLA2 associate differently with oxidative stress and subclinical inflammation in nonobese women (menopausal status.

    Directory of Open Access Journals (Sweden)

    Jean Kyung Paik

    Full Text Available BACKGROUND: This study aimed to determine the association of lipoprotein-associated phospholipase A(2 (Lp-PLA(2 activity in circulation and peripheral blood mononuclear cells (PBMCs with inflammatory and oxidative stress markers in nonobese women and according to menopausal status. Lp-PLA(2 activity, a marker for cardiovascular risk is associated with inflammation and oxidative stress. METHODOLOGY/PRINCIPAL FINDINGS: Eighty postmenopausal women (53.0±4.05 yr and 96 premenopausal women (39.7±9.25 yr participated in this study. Lp-PLA(2 activities, interleukin (IL-6, tumor necrosis factor (TNF-α, and IL-1β in plasma as well as in PBMCs were measured. Plasma ox-LDL was also measured. Postmenopausal women demonstrated higher circulating levels of ox-LDL and IL-6, as well as IL-6, TNF-α, and IL-1β in PBMCs, than premenopausal women. In both groups, plasma Lp-PLA(2 activity positively correlated with Lp-PLA(2 activity in PBMCs and plasma ox-LDL. In premenopausal women, Lp-PLA(2 activities in plasma and PBMCs positively correlated with IL-6, TNF-α, and IL-1β in PBMCs. In postmenopausal women, plasma ox-LDL positively correlated with PBMC cytokine production. In subgroup analysis of postmenopausal women according to plasma ox-LDL level (median level: 48.715 U/L, a significant increase in Lp-PLA(2 activity in the plasma but not the PBMCs was found in the high ox-LDL subgroup. Plasma Lp-PLA(2 activity positively correlated with unstimulated PBMC Lp-PLA(2 activity in the low ox-LDL subgroup (r = 0.627, P<0.001, whereas in the high ox-LDL circulating Lp-PLA(2 activity positively correlated with plasma ox-LDL (r = 0.390, P = 0.014 but not with Lp-PLA(2 activity in PBMCs. CONCLUSIONS/SIGNIFICANCE: The lack of relation between circulating Lp-PLA(2 activity and Lp-PLA(2 activity in PBMCs was found in postmenopausal women with high ox-LDL. This may indicate other sources of circulating Lp-PLA(2 activity except PBMC in postmenopausal women

  12. The antimicrobial lysine-peptoid hybrid LP5 inhibits DNA replication and induces the SOS response in Staphylococcus aureus

    DEFF Research Database (Denmark)

    Gottschalk, Sanne; Ifrah, Dan; Lerche, Sandra

    2013-01-01

    the growth of S. aureus without ATP leakage. Instead, LP5 bound DNA and inhibited macromolecular synthesis. The binding to DNA also led to inhibition of DNA gyrase and topoisomerase IV and caused induction of the SOS response. CONCLUSIONS: Our data demonstrate that LP5 may have a dual mode of action against...... S. aureus. At MIC concentrations, LP5 binds DNA and inhibits macromolecular synthesis and growth, whereas at concentrations above the MIC, LP5 targets the bacterial membrane leading to disruption of the membrane. These results add new information about the MOA of a new synthetic AMP and aid...... in the future design of synthetic peptides with increased therapeutic potential....

  13. Techniques of Ozone Monitoring in a Mountain Forest Region: Passive and Continuous Sampling, Vertical and Canopy Profiles

    Directory of Open Access Journals (Sweden)

    Giacomo Gerosa

    2001-01-01

    Full Text Available Ozone is the most harmful air pollutant for plant ecosystems in the Mediterranean and Alpine areas due to its biological and economic damage to crops and forests. In order to evaluate the relation between ozone exposure and vegetation injury under on-field conditions, suitable ozone monitoring techniques were investi-gated. In the framework of a 5-year research project aimed at ozone risk assessment on forests, both continuous analysers and passive samplers were employed during the summer seasons (1994�1998 in different sites of a wide mountain region (80 x 40 km2 on the southern slope of the European Alps. Continuous analysers allowed the recording of ozone hourly concentration means necessary both to calculate specific exposure indexes (such as AOT, SUM, W126 and to record daily time-courses. Passive samplers, even though supplied only weekly mean concentration values, made it possible to estimate the altitude concentration gradient useful to correct the altitude dependence of ozone concentrations to be inserted into exposure indexes. In-canopy ozone profiles were also determined by placing passive samplers at different heights inside the forest canopy. Vertical ozone soundings by means of tethered balloons (kytoons allowed the measurement of the vertical concentration gradient above the forest canopy. They also revealed ozone reservoirs aloft and were useful to explain the ozone advection dynamic in mountain slopes where ground measurement proved to be inadequate. An intercomparison between passive (PASSAM, CH and continuous measurements highlighted the necessity to accurately standardize all the exposure operations, particularly the pre- and postexposure conservation at cold temperature to avoid dye (DPE activity. Advantages and disadvantages from each mentioned technique are discussed.

  14. Prevalence, Virulence Potential, and Antibiotic Susceptibility Profile of Listeria monocytogenes Isolated From Bovine Raw Milk Samples Obtained From Rajasthan, India.

    Science.gov (United States)

    Sharma, Sanjita; Sharma, Vishnu; Dahiya, Dinesh Kumar; Khan, Aarif; Mathur, Manisha; Sharma, Amit

    2017-03-01

    Listeriosis is a serious foodborne disease of a global concern, and can effectively be controlled by a continuous surveillance of the virulent and multidrug-resistant strains of Listeria monocytogenes. This study was planned to investigate prevalence of L. monocytogenes in bovine raw milk samples. A total of 457 raw milk samples collected from 15 major cities in Rajasthan, India, were analyzed for the presence of L. monocytogenes by using standard microbiological and molecular methods. Five of the 457 samples screen tested positive for L. monocytogenes. Multiplex serotyping showed that 3/5 strains belonged to serotype 4b followed by one strain each to 1/2a and to 1/2c. Further virulence potential assessment indicated that all strains possessed inlA and inlC internalins, and, in addition, two strains also possessed the gene for inlB. All strains were positive for Listeriolysin O (LLO) and showed phosphatidylinositol-specific phospholipase C (PI-PLC) activity on an in vitro agar medium with variations in production levels among the strains. A good correlation between the in vitro pathogenicity test and the chick embryo test was observed, as the strains showing higher LLO and PI-PLC activity were found to be lethal to fertilized chick embryos. All strains were resistant to the majority of antibiotics and were designated as multidrug-resistant strains. However, these strains were susceptible to 9 of the 22 tested antibiotics. The maximum zone of inhibition (mm) and acceptable minimum inhibitory concentration were observed with azithromycin, and thus it could be the first choice of a treatment. Overall, the presence of multidrug-resistant L. monocytogenes strains in the raw milk of Rajasthan region is an indicator of public health hazard and highlighting the need of consumer awareness in place and implementation of stricter food safety regulations at all levels of milk production.

  15. Sequential enzymatic derivatization coupled with online microdialysis sampling for simultaneous profiling of mouse tumor extracellular hydrogen peroxide, lactate, and glucose.

    Science.gov (United States)

    Su, Cheng-Kuan; Tseng, Po-Jen; Chiu, Hsien-Ting; Del Vall, Andrea; Huang, Yu-Fen; Sun, Yuh-Chang

    2017-03-01

    Probing tumor extracellular metabolites is a vitally important issue in current cancer biology. In this study an analytical system was constructed for the in vivo monitoring of mouse tumor extracellular hydrogen peroxide (H2O2), lactate, and glucose by means of microdialysis (MD) sampling and fluorescence determination in conjunction with a smart sequential enzymatic derivatization scheme-involving a loading sequence of fluorogenic reagent/horseradish peroxidase, microdialysate, lactate oxidase, pyruvate, and glucose oxidase-for step-by-step determination of sampled H2O2, lactate, and glucose in mouse tumor microdialysate. After optimization of the overall experimental parameters, the system's detection limit reached as low as 0.002 mM for H2O2, 0.058 mM for lactate, and 0.055 mM for glucose, based on 3 μL of microdialysate, suggesting great potential for determining tumor extracellular concentrations of lactate and glucose. Spike analyses of offline-collected mouse tumor microdialysate and monitoring of the basal concentrations of mouse tumor extracellular H2O2, lactate, and glucose, as well as those after imparting metabolic disturbance through intra-tumor administration of a glucose solution through a prior-implanted cannula, were conducted to demonstrate the system's applicability. Our results evidently indicate that hyphenation of an MD sampling device with an optimized sequential enzymatic derivatization scheme and a fluorescence spectrometer can be used successfully for multi-analyte monitoring of tumor extracellular metabolites in living animals. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. Comparative studies on polyphenolic profile and antimicrobial activity of propolis samples selected from distinctive geographical areas of Hungary.

    Science.gov (United States)

    Molnár, Szabolcs; Mikuska, Kata; Patonay, Katalin; Sisa, Krisztina; Daood, Hussein G; Némedi, Erzsébet; Kiss, Attila

    2017-06-01

    The present paper reports about a comparative survey on the chemical composition, antioxidant activity and in vitro antimicrobial activity of selected propolis samples collected in Hungary. The total levels of polyphenolic compounds including flavonoids in ethanolic extracts of propolis were assessed. The major constituents of ethanolic extracts of propolis were analysed by gas chromatography/mass spectrometry analysis. Total phenolic content was determined spectrophotometrically using a Folin-Ciocalteu reagent. Free radical scavenging activities were evaluated by means of 2,2-diphenyl-1-picrylhydrazyl assay. In vitro inhibitory activity was investigated against eight different bacterial strains by agar well diffusion assay. An extensive comparison was carried out regarding general parameters and specific polyphenolic components. The experimental data led to the observation that there is considerable variability in terms of the quality and the biological value of the distinctive propolis samples. These findings confirm the hypothesis of the study; versatile experimental results are required for proper, well-reasoned, balanced and standardised industrial applications. The major flavonoid components were found to be chrysin and pinocembrin; however, versatile minor components were also detected. The total polyphenol content of ethanolic extracts of propolis ranged between 104.6 mg/g and 286.9 mg/g (gallic acid equivalent). The radical scavenging activity of ethanolic extracts of propolis varied between 101.7 mg/g and 286.9 mg/g (ascorbic acid equivalent). As the quality of propolis depends on the season, vegetation and the area of collection, marked differences were found among the different products examined in terms of both composition and general characteristics. The studied samples exhibited significant differences in term of antimicrobial activities.

  17. Bacteriological Profile and Antimicrobial Susceptibility Patterns of Bacteria Isolated from Pus/Wound Swab Samples from Children Attending a Tertiary Care Hospital in Kathmandu, Nepal

    Directory of Open Access Journals (Sweden)

    Salu Rai

    2017-01-01

    Full Text Available In Nepal, little is known about the microbiological profile of wound infections in children and their antimicrobial susceptibility patterns. Total of 450 pus/wound swab samples collected were cultured using standard microbiological techniques and the colonies grown were identified with the help of biochemical tests. The antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion technique. Methicillin-resistant Staphylococcus aureus isolates were detected by using cefoxitin disc and confirmed by determining minimum inhibitory concentrations (MIC of oxacillin. 264 (59% samples were culture positive. The highest incidence of bacterial infections was noted in the age group of less than 1 year (76%. Out of 264 growth positive samples, Gram-positive bacteria were isolated from 162 (61% samples and Gram-negative bacteria were found in 102 (39% samples. Staphylococcus aureus (99% was the predominant Gram-positive bacteria isolated and Pseudomonas aeruginosa (44% was predominant Gram-negative bacteria. About 19% of S. aureus isolates were found to be methicillin-resistant MIC of oxacillin ranging from 4 μg/mL to 128 μg/mL. Among the children of Nepal, those of age less than 1 year were at higher risk of wound infections by bacteria. Staphylococcus aureus followed by Pseudomonas aeruginosa were the most common bacteria causing wound infections in children.

  18. Potential bacterial core species associated with digital dermatitis in cattle herds identified by molecular profiling of interdigital skin samples

    DEFF Research Database (Denmark)

    Weiss Nielsen, Martin; Strube, Mikael Lenz; Isbrand, Anastasia

    2016-01-01

    of different molecular methods. Deep sequencing of the 16S rRNA gene variable regions V1–V2 showed that Treponema, Mycoplasma, Fusobacterium and Porphyromonas were the genera best differentiating the DD samples from the controls. Additional deep sequencing analysis of the most abundant genus, Treponema......, targeting another variable region of the 16S rRNA gene, V3–V4, identified 15 different phylotypes, among which Treponema phagedenis-like and Treponema refringens-like species were the most abundant. Although the presence of Treponema spp., Fusobacterium necrophorum and Porphyromonas levii was confirmed...

  19. Versatile pulsed laser setup for depth profiling analysis of multilayered samples in the field of cultural heritage

    Science.gov (United States)

    Mendes, N. F. C.; Osticioli, I.; Striova, J.; Sansonetti, A.; Becucci, M.; Castellucci, E.

    2009-04-01

    The present study considers the use of a nanosecond pulsed laser setup capable of performing laser induced breakdown spectroscopy (LIBS) and pulsed Raman spectroscopy for the study of multilayered objects in the field of cultural heritage. Controlled etching using the 4th harmonic 266 nm emission of a Nd:YAG laser source with a 8 ns pulse duration was performed on organic films and mineral strata meant to simulate different sequence of layers usually found in art objects such as in easel and mural paintings. The process of micro ablation coupled with powerful spectroscopic techniques operating with the same laser source, constitutes an interesting alternative to mechanical sampling especially when dealing with artworks such as ceramics and metal works which are problematic due to their hardness and brittleness. Another case is that of valuable pieces where sampling is not an option and the materials to analyse lie behind the surface. The capabilities and limitations of such instrumentation were assessed through several tests in order to characterize the trend of the laser ablation on different materials. Monitored ablation was performed on commercial sheets of polyethylene terephthalate (PET), a standard material of known thickness and mechanical stability, and rabbit glue, an adhesive often used in works of art. Measurements were finally carried out on a specimen with a stratigraphy similar to those found in real mural paintings.

  20. Comprehensive profiling of retroviral integration sites using target enrichment methods from historical koala samples without an assembled reference genome

    Directory of Open Access Journals (Sweden)

    Pin Cui

    2016-03-01

    Full Text Available Background. Retroviral integration into the host germline results in permanent viral colonization of vertebrate genomes. The koala retrovirus (KoRV is currently invading the germline of the koala (Phascolarctos cinereus and provides a unique opportunity for studying retroviral endogenization. Previous analysis of KoRV integration patterns in modern koalas demonstrate that they share integration sites primarily if they are related, indicating that the process is currently driven by vertical transmission rather than infection. However, due to methodological challenges, KoRV integrations have not been comprehensively characterized. Results. To overcome these challenges, we applied and compared three target enrichment techniques coupled with next generation sequencing (NGS and a newly customized sequence-clustering based computational pipeline to determine the integration sites for 10 museum Queensland and New South Wales (NSW koala samples collected between the 1870s and late 1980s. A secondary aim of this study sought to identify common integration sites across modern and historical specimens by comparing our dataset to previously published studies. Several million sequences were processed, and the KoRV integration sites in each koala were characterized. Conclusions. Although the three enrichment methods each exhibited bias in integration site retrieval, a combination of two methods, Primer Extension Capture and hybridization capture is recommended for future studies on historical samples. Moreover, identification of integration sites shows that the proportion of integration sites shared between any two koalas is quite small.

  1. Molecular characterization of Salmonella enterica serotype Enteritidis isolates from food and human samples by serotyping, antimicrobial resistance, plasmid profiling, (GTG5-PCR and ERIC-PCR

    Directory of Open Access Journals (Sweden)

    F. Fardsanei

    2016-11-01

    Full Text Available In recent years, Salmonella enterica serovar Enteritidis has been a primary cause of human salmonellosis in many countries. The major objective of this study was to investigate genetic diversity among Salmonella Enteritidis strains from different origins (food and human by Enterobacterial Repetitive Intergenic Consensus (ERIC -PCR, as well as to assess their plasmid profiling and antimicrobial resistance. A total of 30 Salmonella Enteritidis isolates, 15 from food samples (chicken, lamb, beef and duck meats and 15 from clinical samples were collected in Tehran. Identification of isolates as Salmonella was confirmed by using conventional standard biochemical and serological tests. Multiplex-PCR was used for serotyping of isolates to identify Salmonella Enteritidis. Antimicrobial susceptibility testing to 16 agents founds drug resistance patterns among Salmonella Enteritidis isolates. No resistance was observed to cephalexin, ceftriaxone, ceftazidime and cefotaxime, ciprofloxacin, imipenem or meropenem, chloramphenicol and gentamicin. The highest resistance (96.7% was observed to nitrofurantoin. Seven plasmid profiles (P1–P7 were detected, and a 68-kb plasmid was found in all isolates. Two different primers; ERIC and (GTG5 were used for genotyping, which each produced four profiles. The majority of clinical and food isolates fell into two separate common types (CTs with a similar percentage of 95% by ERIC-PCR. Using primer (GTG5, 29 isolates incorporated in three CTs with 70% of isolates showing a single banding pattern. Limited genetic diversity among human and food isolates of Salmonella Enteritidis may indicate that contaminated foods were possibly the source of human salmonellosis. These results confirmed that ERIC-PCR genotyping has limited discriminatory power for Salmonella Enteritidis of different origin.

  2. Highly sensitive and specific analysis of sterol profiles in biological samples by HPLC-ESI-MS/MS.

    Science.gov (United States)

    Honda, Akira; Miyazaki, Teruo; Ikegami, Tadashi; Iwamoto, Junichi; Yamashita, Kouwa; Numazawa, Mitsuteru; Matsuzaki, Yasushi

    2010-08-01

    High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is a powerful method for the microanalysis of compounds in biological samples. Compared with gas chromatography-mass spectrometry (GC-MS), this method is more broadly applicable to various compounds and usually does not require a derivatization step before analysis. However, when neutral sterols are analyzed, the sensitivities of usual HPLC-MS/MS method are not superior to those of GC-MS because the sterols are relatively resistant to ionization. In this review, we introduce the recent development of HPLC-MS/MS analysis for the quantification of non-cholesterol sterols. By adding an effective derivatization step to the conventional procedure, sterol analysis by HPLC-MS/MS surpassed that obtained by GC-MS in sensitivity. In addition, sufficient specificity of this method was achieved by selected reaction monitoring (SRM) and thorough chromatographic separation of each sterol. Copyright 2010 Elsevier Ltd. All rights reserved.

  3. Trace metal profiles in hair samples from children in urban and rural regions of the United Arab Emirates.

    Science.gov (United States)

    Hasan, M Y; Kosanovic, M; Fahim, M A; Adem, A; Petroianu, G

    2004-06-01

    Pollution has increased with industrialization and humans are subjected to exposure to heavy metals from different environmental sources. In oil-producing countries heavy metals are considered a major threat to the population. Metals such as lead, aluminum, manganese, nickel and cadmium may impact various organs of the body, and controlling their toxicity is crucial for individuals at risk. Previous studies utilized blood levels for monitoring metal toxicity. The current study was designed to investigate exposure to lead, aluminum, manganese, nickel and cadmium using scalp hair. Hair samples were randomly collected from 42 children (aged 6-18 y) representing rural and urban areas of the United Arab Emirates. The rural regions were defined as at least 50 km away from factories or traffic sites. Immediately after cutting, hairs were stored in plastic bags and attached to a questionnaire with the relevant background information. Samples were dried, weighed and sealed with polyethylene envelopes. Following extraction procedures with nitric acid, ICP-MS was utilized for metals determination. The analytical instrument showed a high degree of sensitivity and revealed significant differences between levels of some metals in hairs from rural and urban areas. Children from rural areas had mean hair lead levels (microg/g) of 0.79 + 0.10 whereas children from urban area had higher hair lead levels (3.47 + 0.47). Measuring metals concentration in scalp hair could be a useful method for studying exposure and assessing environmental pollution. Although the technique has the potential of being an effective tool for evaluating extent of pollution and identifying potentially toxic elements, it cannot yet replace the standard procedures of measuring air, water and soil metal content.

  4. Impact of delay to cryopreservation on RNA integrity and genome-wide expression profiles in resected tumor samples.

    Directory of Open Access Journals (Sweden)

    Elodie Caboux

    Full Text Available The quality of tissue samples and extracted mRNA is a major source of variability in tumor transcriptome analysis using genome-wide expression microarrays. During and immediately after surgical tumor resection, tissues are exposed to metabolic, biochemical and physical stresses characterized as "warm ischemia". Current practice advocates cryopreservation of biosamples within 30 minutes of resection, but this recommendation has not been systematically validated by measurements of mRNA decay over time. Using Illumina HumanHT-12 v3 Expression BeadChips, providing a genome-wide coverage of over 24,000 genes, we have analyzed gene expression variation in samples of 3 hepatocellular carcinomas (HCC and 3 lung carcinomas (LC cryopreserved at times up to 2 hours after resection. RNA Integrity Numbers (RIN revealed no significant deterioration of mRNA up to 2 hours after resection. Genome-wide transcriptome analysis detected non-significant gene expression variations of -3.5%/hr (95% CI: -7.0%/hr to 0.1%/hr; p = 0.054. In LC, no consistent gene expression pattern was detected in relation with warm ischemia. In HCC, a signature of 6 up-regulated genes (CYP2E1, IGLL1, CABYR, CLDN2, NQO1, SCL13A5 and 6 down-regulated genes (MT1G, MT1H, MT1E, MT1F, HABP2, SPINK1 was identified (FDR <0.05. Overall, our observations support current recommendation of time to cryopreservation of up to 30 minutes and emphasize the need for identifying tissue-specific genes deregulated following resection to avoid misinterpreting expression changes induced by warm ischemia as pathologically significant changes.

  5. Inhibitors for the bacterial ectonucleotidase Lp1NTPDase from Legionella pneumophila.

    Science.gov (United States)

    Fiene, Amelie; Baqi, Younis; Malik, Enas M; Newton, Patrice; Li, Wenjin; Lee, Sang-Yong; Hartland, Elizabeth L; Müller, Christa E

    2016-09-15

    Legionella pneumophila is an aerobic, Gram-negative bacterium of the genus Legionella, which constitutes the major causative agent of Legionnaires' disease. Recently a nucleoside triphosphate diphosphohydrolase (NTPDase) from L. pneumophila was identified and termed Lp1NTPDase; it was found to be a structural and functional homolog of mammalian NTPDases catalyzing the hydrolysis of ATP to ADP and ADP to AMP. Its activity is believed to contribute to the virulence of Legionella pneumophila. Therefore Lp1NTPDase inhibitors are considered as novel antibacterial drugs. However, only weakly potent compounds are available so far. In the present study, a capillary electrophoresis (CE)-based enzyme assay for monitoring the Lp1NTPDase activity was established. The enzymatic reaction was performed in a test tube followed by separation of substrate and products by CE and subsequent quantification by UV analysis. After kinetic characterization of the enzyme, a series of 1-amino-4-ar(alk)ylamino-2-sulfoanthraquinone derivatives structurally related to the anthraquinone dye Reactive Blue 2, a non-selective ecto-NTPDase inhibitor, was investigated for inhibitory activity on Lp1NTPDase using the CE-based enzyme assay. Derivatives bearing a large lipophilic substituent (e.g., fused aromatic rings) in the 4-position of the 1-amino-2-sulfoanthraquinone showed the highest inhibitory activity. Compounds with IC50 values in the low micromolar range were identified. The most potent inhibitor was 1-amino-4-[phenanthrene-9-yl-amino]-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate (28, PSB-16131), with an IC50-value of 4.24μM. It represents the most potent Lp1NTPDase inhibitor described to date. These findings may serve as a starting point for further optimization. Lp1NTPDase inhibition provides a novel approach for the (immuno)therapy of Legionella infections. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Capillary tone: cyclooxygenase, shear stress, luminal glycocalyx, and hydraulic conductivity (Lp).

    Science.gov (United States)

    Williams, Donna A; Flood, Mary H

    2015-04-01

    Control of capillary hydraulic conductivity (Lp) is the physiological mechanism that underpins systemic hydration. Capillaries form the largest surface of endothelial cells in any species with a cardiovascular system and all capillaries are exposed to the flow-induced force, shear stress (τ). Vasoactive molecules such as prostacyclin (cyclooxygenase product, COX) are released from endothelial cells in response to τ. Little is known about how COX activity impacts capillary Lp. The purpose here was to assess Lp in situ following an acute Δτ stimulus and during COX1/COX2 inhibition. Mesenteric true capillaries (TC) of Rana pipiens (pithed) were cannulated for Lp assessment using the modified Landis technique. Rana were randomized into Control and Test groups. Two capillaries per animal were used (perfusate, 10 mg·mL(-1) BSA/frog Ringer's; superfusate, frog Ringer's or indomethacin (10(-5) mol·L(-1)) mixed in frog Ringer's solution). Three distinct responses of Lp to indomethacin (TC2) were demonstrated (TC1 and TC2 medians: Test Subgroup 1, 3.0 vs. 1.8; Test Subgroup 2, 18.2 vs. 2.2; Test Subgroup 3, 4.2 vs. 10.2 × 10(-7) cm·sec(-1)·cm H2O(-1)). Multiple regression analysis revealed a relationship between capillary Lp and systemic red blood cell concentration or hematocrit, plasma protein concentration, and Δτ (Test Subgroup 1, R(2) = 0.59, P healthy state. Recovering barrier function may be an unrecognized benefit of transfusions during blood loss or edema formation. © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

  7. An LP-based heuristic for the fixed charge transportation problem

    DEFF Research Database (Denmark)

    Klose, Andreas

    2007-01-01

    The fixed charge transportation problem consists in finding a minimum cost network flow from a set of suppliers to a set of customers. Beside costs proportional to quantities transported, transportation costs also include a fixed charge. The paper describes a linear programming based heuristic...... approach for computing lower and upper bounds on the minimal cost. To this end, the LP relaxation is iteratively strengthened by means of adding cuts; in each iteration the current LP solution is then used to guide a local search heuristic. In addition to standard polyhedral cuts as lifted cover...

  8. Long Period (LP) volcanic earthquake source location at Merapi volcano by using dense array technics

    Science.gov (United States)

    Metaxian, Jean Philippe; Budi Santoso, Agus; Laurin, Antoine; Subandriyo, Subandriyo; Widyoyudo, Wiku; Arshab, Ghofar

    2015-04-01

    Since 2010, Merapi shows unusual activity compared to last decades. Powerful phreatic explosions are observed; some of them are preceded by LP signals. In the literature, LP seismicity is thought to be originated within the fluid, and therefore to be representative of the pressurization state of the volcano plumbing system. Another model suggests that LP events are caused by slow, quasi-brittle, low stress-drop failure driven by transient upper-edifice deformations. Knowledge of the spatial distribution of LP events is fundamental for better understanding the physical processes occurring in the conduit, as well as for the monitoring and the improvement of eruption forecasting. LP events recorded at Merapi have a spectral content dominated by frequencies between 0.8 and 3 Hz. To locate the source of these events, we installed a seismic antenna composed of 4 broadband CMG-6TD Güralp stations. This network has an aperture of 300 m. It is located on the site of Pasarbubar, between 500 and 800 m from the crater rim. Two multi-parameter stations (seismic, tiltmeter, S-P) located in the same area, equipped with broadband CMG-40T Güralp sensors may also be used to complete the data of the antenna. The source of LP events is located by using different approaches. In the first one, we used a method based on the measurement of the time delays between the early beginnings of LP events for each array receiver. The observed differences of time delays obtained for each pair of receivers are compared to theoretical values calculated from the travel times computed between grid nodes, which are positioned in the structure, and each receiver. In a second approach, we estimate the slowness vector by using MUSIC algorithm applied to 3-components data. From the slowness vector, we deduce the back-azimuth and the incident angle, which give an estimation of LP source depth in the conduit. This work is part of the Domerapi project funded by French Agence Nationale de la Recherche (https

  9. High Throughput qPCR Expression Profiling of Circulating MicroRNAs Reveals Minimal Sex- and Sample Timing-Related Variation in Plasma of Healthy Volunteers.

    Science.gov (United States)

    Mooney, Catherine; Raoof, Rana; El-Naggar, Hany; Sanz-Rodriguez, Amaya; Jimenez-Mateos, Eva M; Henshall, David C

    2015-01-01

    MicroRNAs are a class of small non-coding RNA that regulate gene expression at a post-transcriptional level. MicroRNAs have been identified in various body fluids under normal conditions and their stability as well as their dysregulation in disease opens up a new field for biomarker study. However, diurnal and day-to-day variation in plasma microRNA levels, and differential regulation between males and females, may affect biomarker stability. A QuantStudio 12K Flex Real-Time PCR System was used to profile plasma microRNA levels using OpenArray in male and female healthy volunteers, in the morning and afternoon, and at four time points over a one month period. Using this system we were able to run four OpenArray plates in a single run, the equivalent of 32 traditional 384-well qPCR plates or 12,000 data points. Up to 754 microRNAs can be identified in a single plasma sample in under two hours. 108 individual microRNAs were identified in at least 80% of all our samples which compares favourably with other reports of microRNA profiles in serum or plasma in healthy adults. Many of these microRNAs, including miR-16-5p, miR-17-5p, miR-19a-3p, miR-24-3p, miR-30c-5p, miR-191-5p, miR-223-3p and miR-451a are highly expressed and consistent with previous studies using other platforms. Overall, microRNA levels were very consistent between individuals, males and females, and time points and we did not detect significant differences in levels of microRNAs. These results suggest the suitability of this platform for microRNA profiling and biomarker discovery and suggest minimal confounding influence of sex or sample timing. However, the platform has not been subjected to rigorous validation which must be demonstrated in future biomarker studies where large differences may exist between disease and control samples.

  10. High Throughput qPCR Expression Profiling of Circulating MicroRNAs Reveals Minimal Sex- and Sample Timing-Related Variation in Plasma of Healthy Volunteers.

    Directory of Open Access Journals (Sweden)

    Catherine Mooney

    Full Text Available MicroRNAs are a class of small non-coding RNA that regulate gene expression at a post-transcriptional level. MicroRNAs have been identified in various body fluids under normal conditions and their stability as well as their dysregulation in disease opens up a new field for biomarker study. However, diurnal and day-to-day variation in plasma microRNA levels, and differential regulation between males and females, may affect biomarker stability. A QuantStudio 12K Flex Real-Time PCR System was used to profile plasma microRNA levels using OpenArray in male and female healthy volunteers, in the morning and afternoon, and at four time points over a one month period. Using this system we were able to run four OpenArray plates in a single run, the equivalent of 32 traditional 384-well qPCR plates or 12,000 data points. Up to 754 microRNAs can be identified in a single plasma sample in under two hours. 108 individual microRNAs were identified in at least 80% of all our samples which compares favourably with other reports of microRNA profiles in serum or plasma in healthy adults. Many of these microRNAs, including miR-16-5p, miR-17-5p, miR-19a-3p, miR-24-3p, miR-30c-5p, miR-191-5p, miR-223-3p and miR-451a are highly expressed and consistent with previous studies using other platforms. Overall, microRNA levels were very consistent between individuals, males and females, and time points and we did not detect significant differences in levels of microRNAs. These results suggest the suitability of this platform for microRNA profiling and biomarker discovery and suggest minimal confounding influence of sex or sample timing. However, the platform has not been subjected to rigorous validation which must be demonstrated in future biomarker studies where large differences may exist between disease and control samples.

  11. Genome-wide association study on detailed profiles of smoking behavior and nicotine dependence in a twin sample.

    Science.gov (United States)

    Loukola, A; Wedenoja, J; Keskitalo-Vuokko, K; Broms, U; Korhonen, T; Ripatti, S; Sarin, A-P; Pitkäniemi, J; He, L; Häppölä, A; Heikkilä, K; Chou, Y-L; Pergadia, M L; Heath, A C; Montgomery, G W; Martin, N G; Madden, P A F; Kaprio, J

    2014-05-01

    Smoking is a major risk factor for several somatic diseases and is also emerging as a causal factor for neuropsychiatric disorders. Genome-wide association (GWA) and candidate gene studies for smoking behavior and nicotine dependence (ND) have disclosed too few predisposing variants to account for the high estimated heritability. Previous large-scale GWA studies have had very limited phenotypic definitions of relevance to smoking-related behavior, which has likely impeded the discovery of genetic effects. We performed GWA analyses on 1114 adult twins ascertained for ever smoking from the population-based Finnish Twin Cohort study. The availability of 17 smoking-related phenotypes allowed us to comprehensively portray the dimensions of smoking behavior, clustered into the domains of smoking initiation, amount smoked and ND. Our results highlight a locus on 16p12.3, with several single-nucleotide polymorphisms (SNPs) in the vicinity of CLEC19A showing association (Psmoking quantity. Interestingly, CLEC19A is located close to a previously reported attention-deficit hyperactivity disorder (ADHD) linkage locus and an evident link between ADHD and smoking has been established. Intriguing preliminary association (PMental Disorders, 4th edition) ND diagnosis and several SNPs in ERBB4, coding for a Neuregulin receptor, on 2q33. The association between ERBB4 and DSM-IV ND diagnosis was replicated in an independent Australian sample. Recently, a significant increase in ErbB4 and Neuregulin 3 (Nrg3) expression was revealed following chronic nicotine exposure and withdrawal in mice and an association between NRG3 SNPs and smoking cessation success was detected in a clinical trial. ERBB4 has previously been associated with schizophrenia; further, it is located within an established schizophrenia linkage locus and within a linkage locus for a smoker phenotype identified in this sample. In conclusion, we disclose novel tentative evidence for the involvement of ERBB4 in ND

  12. Slow Magic Angle Sample Spinning: A Non- or Minimally Invasive Method for High- Resolution 1H Nuclear Magnetic Resonance (NMR) Metabolic Profiling

    Energy Technology Data Exchange (ETDEWEB)

    Hu, Jian Z.

    2011-05-01

    High resolution 1H magic angle spinning nuclear magnetic resonance (NMR), using a sample spinning rate of several kHz or more (i.e., high resolution-magic angle spinning (hr-MAS)), is a well established method for metabolic profiling in intact tissues without the need for sample extraction. The only shortcoming with hr-MAS is that it is invasive and is thus unusable for non-destructive detections. Recently, a method called slow-MAS, using the concept of two dimensional NMR spectroscopy, has emerged as an alternative method for non- or minimal invasive metabolomics in intact tissues, including live animals, due to the slow or ultra-slow-sample spinning used. Although slow-MAS is a powerful method, its applications are hindered by experimental challenges. Correctly designing the experiment and choosing the appropriate slow-MAS method both require a fundamental understanding of the operation principles, in particular the details of line narrowing due to the presence of molecular diffusion. However, these fundamental principles have not yet been fully disclosed in previous publications. The goal of this chapter is to provide an in depth evaluation of the principles associated with slow-MAS techniques by emphasizing the challenges associated with a phantom sample consisting of glass beads and H2O, where an unusually large magnetic susceptibility field gradient is obtained.

  13. A spinach O-acetylserine(thiollyase homologue, SoCSaseLP, suppresses cysteine biosynthesis catalysed by other enzyme isoforms

    Directory of Open Access Journals (Sweden)

    Miki Noda

    2016-06-01

    Full Text Available An enzyme, O-acetylserine(thiollyase (OASTL, also known as O-acetylserine sulfhydrylase or cysteine synthase (CSase, catalyses the incorporation of sulfide into O-acetylserine and produces cysteine. We previously identified a cDNA encoding an OASTL-like protein from Spinacia oleracea, (SoCSaseLP, but a recombinant SoCSaseLP produced in Escherichia coli did not show OASTL activity. The exon-intron structure of the SoCSaseLP gene shared conserved structures with other spinach OASTL genes. The SoCSaseLP and a Beta vulgaris homologue protein, KMT13462, comprise a unique clade in the phylogenetic tree of the OASTL family. Interestingly, when the SoCSaseLP gene was expressed in tobacco plants, total OASTL activity in tobacco leaves was reduced. This reduction in total OASTL activity was most likely caused by interference by SoCSaseLP with cytosolic OASTL. To investigate the possible interaction of SoCSaseLP with a spinach cytosolic OASTL isoform SoCSaseA, a pull-down assay was carried out. The recombinant glutathione S-transferase (GST-SoCSaseLP fusion protein was expressed in E. coli together with the histidine-tagged SoCSaseA protein, and the protein extract was subjected to glutathione affinity chromatography. The histidine-tagged SoCSaseA was co-purified with the GST-SoCSaseLP fusion protein, indicating the binding of SoCSaseLP to SoCSaseA. Consistent with this interaction, the OASTL activity of the co-purified SoCSaseA was reduced compared with the activity of SoCSaseA that was purified on its own. These results strongly suggest that SoCSaseLP negatively regulates the activity of other cytosolic OASTL family members by direct interaction.

  14. Association of carotid plaque Lp-PLA(2 with macrophages and Chlamydia pneumoniae infection among patients at risk for stroke.

    Directory of Open Access Journals (Sweden)

    Berna Atik

    Full Text Available BACKGROUND: We previously showed that the burden of Chlamydia pneumoniae in carotid plaques was significantly associated with plaque interleukin (IL-6, and serum IL-6 and C-reactive protein (CRP, suggesting that infected plaques contribute to systemic inflammatory markers in patients with stroke risk. Since lipoprotein-associated phospholipase A2 (Lp-PLA(2 mediates inflammation in atherosclerosis, we hypothesized that serum Lp-PLA(2 mass and activity levels and plaque Lp-PLA(2 may be influenced by plaque C. pneumoniae infection. METHODOLOGY/PRINCIPAL FINDINGS: Forty-two patients underwent elective carotid endarterectomy. Tissue obtained at surgery was stained by immunohistochemistry for Lp-PLA(2 grade, macrophages, IL-6, C. pneumoniae and CD4+ and CD8+ cells. Serum Lp-PLA(2 activity and mass were measured using the colorimetric activity method (CAM and ELISA, respectively. Serum homocysteine levels were measured by HPLC. Eleven (26.2% patients were symptomatic with transient ischemic attacks. There was no correlation between patient risk factors (smoking, coronary artery disease, elevated cholesterol, diabetes, obesity, hypertension and family history of genetic disorders for atherosclerosis and serum levels or plaque grade for Lp-PLA(2. Plaque Lp-PLA(2 correlated with serum homocysteine levels (p = 0.013, plaque macrophages (p<0.01, and plaque C. pneumoniae (p<0.001, which predominantly infected macrophages, co-localizing with Lp-PLA(2. CONCLUSIONS: The significant association of plaque Lp-PLA(2 with plaque macrophages and C. pneumoniae suggests an interactive role in accelerating inflammation in atherosclerosis. A possible mechanism for C. pneumoniae in the atherogenic process may involve infection of macrophages that induce Lp-PLA(2 production leading to upregulation of inflammatory mediators in plaque tissue. Additional in vitro and in vivo research will be needed to advance our understanding of specific C. pneumoniae and Lp-PLA(2

  15. Sample-directed pseudotargeted method for the metabolic profiling analysis of rice seeds based on liquid chromatography with mass spectrometry.

    Science.gov (United States)

    Zhang, Junjie; Zhao, Chunxia; Zeng, Zhongda; Luo, Ping; Zhao, Yanni; Zhao, Jieyu; Li, Lili; Lu, Xin; Xu, Guowang

    2016-01-01

    Rice is one of the most important food crops in the world. Metabolite composition in rice seeds varies significantly depending on genetic variety, climatic alternation and agricultural practice. Metabolomics is a powerful tool to reveal the metabolic response of rice to various conditions. In this work, a rice seed sample-directed pseudotargeted metabolomics method was first established and validated based on ultra high performance liquid chromatography with triple quadrupole mass spectrometry in the multiple reaction monitoring mode. A total of 749 and 617 ion pairs in positive and negative modes were achieved, respectively. Among them, about 200 metabolites were identified or tentatively identified. The developed method showed better linearity and repeatability than those of non-targeted metabolomics method. Good intra-day and inter-day precisions, recoveries and wide linear range were also obtained. Furthermore, the method was applied for the investigation of metabolic variation of rice seeds with two wild cultivars and their transgenic lines that were grown in two locations. Principal component analysis indicated that the effects of cultivar and location on metabolic variations were far more than those of gene modification. The nonparametric Mann-Whitney U test revealed that most metabolites were influenced by cultivar, location and gene modifications together. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Physicochemical characteristics and sensory profile of honey samples from stingless bees (Apidae: Meliponinae submitted to a dehumidification process

    Directory of Open Access Journals (Sweden)

    Carlos A.L. Carvalho

    2009-03-01

    Full Text Available This study was conducted to evaluate the effect of a dehumidification process on the physicochemical and sensory characteristics of stingless-bee honey. Melipona scutellaris and M. quadrifasciata honey samples were submitted to a dehumidification process and to physicochemical (reducing sugars, apparent sucrose, moisture, diastatic activity, hydroxymethylfurfural, ash, pH, acidity, and electric conductivity and sensory evaluations (fluidity, color, aroma, crystallization,flavor,and acceptability. The results indicated that the dehumidification process does not interfere with honey quality and acceptability.Este estudo foi conduzido com o objetivo de avaliar o efeito do processo de desumidificação sobre as características físico-químicas e sensoriais do mel das abelhas sem ferrão. Amostras de méis de Melipona scutellaris e M. quadrifasciata foram submetidas ao processo de desumidificação, passando em seguida por avaliações físico-químicas (açúcares redutores, sacarose aparente, umidade, atividade diastásica, hidroximetilfurfural, cinzas, pH, acidez e condutividade elétrica e sensoriais (fluidez, cor, aroma, cristalização, sabor e aceitabilidade. Os resultados indicaram que o processo de desumidificação não interfere na qualidade e aceitabilidade do mel.

  17. Virome profiling of bats from Myanmar by metagenomic analysis of tissue samples reveals more novel Mammalian viruses.

    Directory of Open Access Journals (Sweden)

    Biao He

    Full Text Available Bats are reservoir animals harboring many important pathogenic viruses and with the capability of transmitting these to humans and other animals. To establish an effective surveillance to monitor transboundary spread of bat viruses between Myanmar and China, complete organs from the thorax and abdomen from 853 bats of six species from two Myanmar counties close to Yunnan province, China, were collected and tested for their virome through metagenomics by Solexa sequencing and bioinformatic analysis. In total, 3,742,314 reads of 114 bases were generated, and over 86% were assembled into 1,649,512 contigs with an average length of 114 bp, of which 26,698 (2% contigs were recognizable viral sequences belonging to 24 viral families. Of the viral contigs 45% (12,086/26,698 were related to vertebrate viruses, 28% (7,443/26,698 to insect viruses, 27% (7,074/26,698 to phages and 95 contigs to plant viruses. The metagenomic results were confirmed by PCR of selected viruses in all bat samples followed by phylogenetic analysis, which has led to the discovery of some novel bat viruses of the genera Mamastrovirus, Bocavirus, Circovirus, Iflavirus and Orthohepadnavirus and to their prevalence rates in two bat species. In conclusion, the present study aims to present the bat virome in Myanmar, and the results obtained further expand the spectrum of viruses harbored by bats.

  18. Isotope-coded ESI-enhancing derivatization reagents for differential analysis, quantification and profiling of metabolites in biological samples by LC/MS: A review.

    Science.gov (United States)

    Higashi, Tatsuya; Ogawa, Shoujiro

    2016-10-25

    The analysis of the qualitative and quantitative changes of metabolites in body fluids and tissues yields valuable information for the diagnosis, pathological analysis and treatment of many diseases. Recently, liquid chromatography/electrospray ionization-(tandem) mass spectrometry [LC/ESI-MS(/MS)] has been widely used for these purposes due to the high separation capability of LC, broad coverage of ESI for various compounds and high specificity of MS(/MS). However, there are still two major problems to be solved regarding the biological sample analysis; lack of sensitivity and limited availability of stable isotope-labeled analogues (internal standards, ISs) for most metabolites. Stable isotope-coded derivatization (ICD) can be the answer for these problems. By the ICD, different isotope-coded moieties are introduced to the metabolites and one of the resulting derivatives can serve as the IS, which minimize the matrix effects. Furthermore, the derivatization can improve the ESI efficiency, fragmentation property in the MS/MS and chromatographic behavior of the metabolites, which lead to a high sensitivity and specificity in the various detection modes. Based on this background, this article reviews the recently-reported isotope-coded ESI-enhancing derivatization (ICEED) reagents, which are key components for the ICD-based LC/MS(/MS) studies, and their applications to the detection, identification, quantification and profiling of metabolites in human and animal samples. The LC/MS(/MS) using the ICEED reagents is the powerful method especially for the differential analysis (relative quantification) of metabolites in two comparative samples, simultaneous quantification of multiple metabolites whose stable isotope-labeled ISs are not available, and submetabolome profiling. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. Cytokine and hormonal profile in serum samples of patients undergoing controlled ovarian stimulation: interleukin-1beta predicts ongoing pregnancy.

    Science.gov (United States)

    Bonetti, T C S; Salomao, R; Brunialti, M; Braga, D P A F; Borges, E; Silva, I D C G

    2010-08-01

    Changes in the endometrium are not regulated exclusively by ovarian hormones; the immune system has also been implicated in normal endometrial function, similar to processes taking place during inflammatory and reparative path. Many cytokines are crucially important for reproductive processes, and the role of cytokines in the female reproductive system function has been broadly investigated during controlled ovarian stimulation (COS) for IVF attempts. The aim of this study was to evaluate the levels of serum cytokines and hormones, and the clinical outcomes of women who underwent COS and ICSI procedures. The study prospectively included 96 patients (aged 22-43 years, unexplained or male infertility, n = 61; female infertility factors, n = 35) who underwent ICSI cycles. Serum levels of interleukin (IL-8, IL-6, IL-1beta, IL-10, IL-12), tumour necrosis factor and leukaemia-inhibitory factor (LIF) and the hormones FSH, estradiol, progesterone, anti-Mullerian hormone and Inhibin-B were measured on the day of oocyte retrieval. The ongoing pregnancy rate was 25.3%. The presence of serum IL-1beta positively affected the implantation rate (P = 0.004) and increased the chance of becoming pregnant by 15 fold. Furthermore, the percentage of patients with detectable serum IL-1beta levels who conceived (62.5%) was higher than those who failed to conceive (37.5%; P = 0.019). The LIF was undetectable in all serum samples, and no other factors influenced the clinical outcomes of patients undergoing ICSI cycles. Our findings revealed that detectable serum levels of IL-1beta on the day of oocyte retrieval in patients undergoing COS and ICSI are predictive of successful implantation and ongoing pregnancy.

  20. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the OCEANUS in the North Atlantic Ocean from 2008-05-10 to 2008-05-23 (NODC Accession 0117673)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117673 includes chemical, discrete sample, physical and profile data collected from OCEANUS in the North Atlantic Ocean from 2008-05-10 to 2008-05-23...

  1. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from USCGC POLAR STAR in the Arctic Ocean from 2002-08-19 to 2002-09-23 (NODC Accession 0115588)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0115588 includes discrete sample and profile data collected from USCGC POLAR STAR in the Arctic Ocean from 2002-08-19 to 2002-09-23. These data...

  2. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the POLARSTERN in the South Atlantic Ocean from 1989-09-06 to 1989-10-30 (NODC Accession 0116645)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116645 includes chemical, discrete sample, physical and profile data collected from POLARSTERN in the South Atlantic Ocean from 1989-09-06 to...

  3. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the SHOYO in the North Pacific Ocean from 1993-10-14 to 1993-11-27 (NODC Accession 0115607)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115607 includes chemical, discrete sample, physical and profile data collected from SHOYO in the North Pacific Ocean from 1993-10-14 to 1993-11-27...

  4. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the JAN MAYEN in the Norwegian Sea from 1993-04-01 to 1995-11-01 (NODC Accession 0115677)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115677 includes chemical, discrete sample, physical and profile data collected from JAN MAYEN in the Norwegian Sea from 1993-04-01 to 1995-11-01 and...

  5. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the KNORR in the North Atlantic Ocean from 1986-04-24 to 1986-05-18 (NODC Accession 0117678)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117678 includes chemical, discrete sample, physical and profile data collected from KNORR in the North Atlantic Ocean from 1986-04-24 to 1986-05-18...

  6. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the KNORR in the South Pacific Ocean from 2006-01-30 to 2006-03-14 (NODC Accession 0115593)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115593 includes chemical, discrete sample, physical and profile data collected from KNORR in the South Pacific Ocean from 2006-01-30 to 2006-03-14...

  7. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the AURORA AUSTRALIS in the Indian Ocean from 1991-09-25 to 1991-10-27 (NODC Accession 0116370)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116370 includes chemical, discrete sample, physical and profile data collected from AURORA AUSTRALIS in the Indian Ocean from 1991-09-25 to...

  8. PH, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from DISCOVERY in the North Atlantic Ocean from 1998-04-23 to 1998-06-01 (NODC Accession 0113536)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0113536 includes biological, chemical, discrete sample, physical and profile data collected from DISCOVERY in the North Atlantic Ocean from 1998-04-23...

  9. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from CHARLES DARWIN in the Indian Ocean from 1987-11-12 to 1987-12-17 (NCEI Accession 0157468)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0157468 includes chemical, discrete sample, physical and profile data collected from CHARLES DARWIN in the Indian Ocean from 1987-11-12 to 1987-12-17....

  10. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the DISCOVERY in the North Atlantic Ocean from 1989-07-16 to 1989-08-10 (NODC Accession 0113532)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113532 includes chemical, discrete sample, physical and profile data collected from DISCOVERY in the North Atlantic Ocean from 1989-07-16 to...

  11. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the METEOR in the North Atlantic Ocean from 2003-06-26 to 2003-07-21 (NODC Accession 0115682)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115682 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 2003-06-26 to 2003-07-21...

  12. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the Hakuho Maru in the North Pacific Ocean from 1993-05-13 to 1993-05-30 (NODC Accession 0115496)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115496 includes chemical, discrete sample, physical and profile data collected from Hakuho Maru in the North Pacific Ocean from 1993-05-13 to...

  13. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from RYOFU MARU in the Philippine Sea from 1992-02-10 to 1992-02-17 (NCEI Accession 0143943)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0143943 includes discrete sample and profile data collected from RYOFU MARU in the Philippine Sea from 1992-02-10 to 1992-02-17. These data include...

  14. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the DARVIN in the North Atlantic Ocean from 1991-07-12 to 1991-07-24 (NODC Accession 0113526)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113526 includes chemical, discrete sample, physical and profile data collected from DARVIN in the North Atlantic Ocean from 1991-07-12 to 1991-07-24...

  15. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the RYOFU MARU in the Philippine Sea from 1995-11-15 to 1995-11-30 (NODC Accession 0117669)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0117669 includes chemical, discrete sample, physical and profile data collected from RYOFU MARU in the Philippine Sea from 1995-11-15 to 1995-11-30...

  16. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from DISCOVERY in the Indian Ocean from 1995-01-06 to 1995-02-21 (NCEI Accession 0160543)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0160543 includes biological, chemical, discrete sample, physical and profile data collected from DISCOVERY in the Indian Ocean from 1995-01-06 to...

  17. PH, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from SHUMPU MARU in the Philippine Sea from 1992-02-25 to 1992-02-29 (NODC Accession 0115709)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0115709 includes chemical, discrete sample, physical and profile data collected from SHUMPU MARU in the Philippine Sea from 1992-02-25 to 1992-02-29....

  18. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from ATLANTIS in the North Atlantic Ocean from 2006-01-18 to 2006-01-31 (NCEI Accession 0157469)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0157469 includes chemical, discrete sample, physical and profile data collected from ATLANTIS in the North Atlantic Ocean from 2006-01-18 to...

  19. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the OCEAN RESEARCHER I in the Philippine Sea from 1991-06-26 to 1991-07-04 (NODC Accession 0115598)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115598 includes chemical, discrete sample, physical and profile data collected from OCEAN RESEARCHER I in the Philippine Sea from 1991-06-26 to...

  20. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from Kaiyo in the Philippine Sea from 1994-02-12 to 1994-02-16 (NCEI Accession 0143635)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0143635 includes discrete sample and profile data collected from Kaiyo in the Philippine Sea from 1994-02-12 to 1994-02-16. These data include DELTA...

  1. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from DISCOVERY in the South Atlantic Ocean from 1992-12-22 to 1993-02-01 (NCEI Accession 0144287)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0144287 includes discrete sample and profile data collected from DISCOVERY in the South Atlantic Ocean from 1992-12-22 to 1993-02-01. These data...

  2. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from PROFESSOR MULTANOVSKIY in the North Atlantic Ocean from 1993-09-11 to 1993-11-21 (NCEI Accession 0143931)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0143931 includes discrete sample and profile data collected from PROFESSOR MULTANOVSKIY in the North Atlantic Ocean from 1993-09-11 to 1993-11-21....

  3. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the OCEAN RESEARCHER I in the Philippine Sea from 1990-10-11 to 1990-10-15 (NODC Accession 0115600)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115600 includes chemical, discrete sample, physical and profile data collected from OCEAN RESEARCHER I in the Philippine Sea from 1990-10-11 to...

  4. PH, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from THALASSA in the North Atlantic Ocean from 2004-06-04 to 2004-07-06 (NODC Accession 0113918)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0113918 includes chemical, discrete sample, physical and profile data collected from THALASSA in the North Atlantic Ocean from 2004-06-04 to...

  5. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from THOMAS G. THOMPSON in the North Pacific Ocean from 1985-08-04 to 1985-09-07 (NCEI Accession 0143394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0143394 includes discrete sample and profile data collected from THOMAS G. THOMPSON in the North Pacific Ocean from 1985-08-04 to 1985-09-07 and...

  6. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the METEOR in the North Atlantic Ocean from 1999-08-13 to 1999-08-31 (NODC Accession 0115603)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115603 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 1999-08-13 to 1999-08-31...

  7. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from DISCOVERY in the Indian Ocean and Southern Oceans from 1994-02-19 to 1994-03-30 (NCEI Accession 0144242)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0144242 includes discrete sample and profile data collected from DISCOVERY in the Indian Ocean and Southern Oceans (> 60 degrees South) from...

  8. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the NEW HORIZON in the North Pacific Ocean from 1980-08-10 to 1980-09-03 (NODC Accession 0116707)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0116707 includes chemical, discrete sample, physical and profile data collected from NEW HORIZON in the North Pacific Ocean from 1980-08-10 to...

  9. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from THOMAS G. THOMPSON in the South Pacific Ocean from 2013-10-25 to 2013-12-20 (NCEI Accession 0163186)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NCEI Accession 0163186 includes chemical, discrete sample, physical and profile data collected from THOMAS G. THOMPSON in the South Pacific Ocean from 2013-10-25 to...

  10. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the METEOR in the North Atlantic Ocean from 2001-06-20 to 2001-07-15 (NODC Accession 0115601)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0115601 includes chemical, discrete sample, physical and profile data collected from METEOR in the North Atlantic Ocean from 2001-06-20 to 2001-07-15...

  11. Temperature, salinity and other variables collected from discrete sample and profile observations using CTD, bottle and other instruments from the ENDEAVOR in the North Atlantic Ocean from 1991-03-28 to 1991-04-21 (NODC Accession 0113988)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0113988 includes chemical, discrete sample, physical and profile data collected from ENDEAVOR in the North Atlantic Ocean from 1991-03-28 to...

  12. Efficacy and Safety of Lactobacillus plantarum DSM 9843 (LP299V) in the Prevention of Antibiotic-Associated Gastrointestinal Symptoms in Children-Randomized, Double-Blind, Placebo-Controlled Study.

    Science.gov (United States)

    Olek, Agnieszka; Woynarowski, Marek; Ahrén, Irini Lazou; Kierkuś, Jarosław; Socha, Piotr; Larsson, Niklas; Önning, Gunilla

    2017-07-01

    To determine if Lactobacillus plantarum DSM9843 (LP299V) reduces the frequency of antibiotic-associated loose/watery stools and gastrointestinal symptoms, and can be administered safely to children who are prescribed antibiotics. We performed a prospective, double-blind, randomized, placebo-controlled, multicenter, parallel-group study in children receiving outpatient antibiotic therapy in primary healthcare settings. The children were given LP299V/placebo during the antibiotic therapy and for 1 week after the end of treatment. The primary outcome measure was the incidence of at least 1 loose/watery stool (type 6 or 7 according to the Bristol Stool Form Scale). Gastrointestinal symptoms (abdominal pain, abdominal distention, vomiting, and flatulence) were followed up until 1 week after the last intake of the study product. A total of 438 children (male: 235, female: 203) aged 1-11 years (mean ± SD: 5.2 ± 2.7) were randomized to receive LP299V (N = 218) or placebo (N = 220). The incidence of loose/watery stools in the 2 study groups (LP299V and placebo) was similar, 39% vs 44.5% respectively (P = .26) as was the mean number of loose/watery stools (3.9 ± 3.5 vs 4.7 ± 6.3; P = .9). Antibiotic-associated diarrhea (defined as ≥3 loose/watery stools/24 hours starting from 2 hours after initiation of antibiotic treatment until the end of the study) occurred in 2.8% of the subjects receiving LP299V compared with 4.1% in the placebo arm (P = .4). The number of children with abdominal symptoms did not differ between the groups. No beneficial effect of LP299V compared with placebo was observed for the incidence of loose/watery stools, mean number of loose/watery stools, or the incidence of abdominal symptoms. LP299V had a satisfactory safety profile. ClinicalTrials.gov: NCT01940913. Copyright © 2017 Elsevier Inc. All rights reserved.

  13. Primary health clinic toilet/bathroom surface swab sampling can indicate community profile of sexually transmitted infections

    Science.gov (United States)

    Su, Jiunn-Yih; Andersson, Patiyan; Holt, Deborah C.

    2017-01-01

    relative prevalence of gonorrhoeae in central Australia than in northern Australia. Similarly, the regional clinics yielded p values from 0.0088–0.0022. In contrast, swab and notifications data from the sexual health clinic were not correlated. Discussion Strong correlations between swab and notifications were observed. However, there was evidence for limitations of this approach. Despite the correlation observed with the regional clinics data, one clinic yielded zero positive swabs for C. trachomatis, although this STI constituted 25.1% of the corresponding notifications. This could be ascribed to stochastic effects. The lack of correlation observed for sexual health clinic data was also likely due to stochastic effects. It was concluded that toilet/bathroom surface swab sampling has considerable potential for public health surveillance. The approach may be applicable in situations other than primary health clinics, and for targets other than STIs. PMID:28652937

  14. Primary health clinic toilet/bathroom surface swab sampling can indicate community profile of sexually transmitted infections

    Directory of Open Access Journals (Sweden)

    Philip M. Giffard

    2017-06-01

    known higher relative prevalence of gonorrhoeae in central Australia than in northern Australia. Similarly, the regional clinics yielded p values from 0.0088–0.0022. In contrast, swab and notifications data from the sexual health clinic were not correlated. Discussion Strong correlations between swab and notifications were observed. However, there was evidence for limitations of this approach. Despite the correlation observed with the regional clinics data, one clinic yielded zero positive swabs for C. trachomatis, although this STI constituted 25.1% of the corresponding notifications. This could be ascribed to stochastic effects. The lack of correlation observed for sexual health clinic data was also likely due to stochastic effects. It was concluded that toilet/bathroom surface swab sampling has considerable potential for public health surveillance. The approach may be applicable in situations other than primary health clinics, and for targets other than STIs.

  15. 75 FR 8921 - Grant of Authority for Subzone Status; Brightpoint North America L.P. (Cell Phone Kitting and...

    Science.gov (United States)

    2010-02-26

    ... authority to establish a special-purpose subzone at the cell phone kitting and distribution facilities of... kitting and distribution of cell phones at the facilities of Brightpoint North America L.P., located in... Foreign-Trade Zones Board Grant of Authority for Subzone Status; Brightpoint North America L.P. (Cell...

  16. 75 FR 39244 - Arizona Public Service Company, Sequent Energy Management, L.P.; Notice of Joint Petition for...

    Science.gov (United States)

    2010-07-08

    ... Energy Regulatory Commission Arizona Public Service Company, Sequent Energy Management, L.P.; Notice of... notice that on June 25, 2010, Arizona Public Service Company and Sequent Energy Management, L.P. filed...://www.ferc.gov , using the ``eLibrary'' link and is available for review in the Commission's Public...

  17. 75 FR 49926 - Arena Energy, L.P. v. Sea Robin Pipeline Company, LLC; Notice of Complaint

    Science.gov (United States)

    2010-08-16

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Arena Energy, L.P. v. Sea Robin Pipeline Company, LLC; Notice of Complaint... Commission's (Commission) Rules of Practice and Procedure, 18 CFR 385.206 (2010), Arena Energy, L.P...

  18. Prenatal exposure to a maternal LP diet decreases BDNF expression in the brains of the neonatal offspring

    Science.gov (United States)

    Maternal low protein (LP) diets during gestation cause learning and mernmy impai1ment as well as cognitive deficits in the neonatal and adult offsp1ing. The cellular and molecular mechanism that mediate the deleterious effects of prenatal exposure to a maternal LP diet on cognitive function is egreg...

  19. 75 FR 56093 - Eagle Rock Desoto Pipeline, LP; Notice of Motion for Extension of Rate Case Filing Deadline

    Science.gov (United States)

    2010-09-15

    ... Energy Regulatory Commission Eagle Rock Desoto Pipeline, LP; Notice of Motion for Extension of Rate Case Filing Deadline September 8, 2010. Take notice that on September 8, 2010, Eagle Rock Desoto Pipeline, L.P. (Eagle Rock) filed a request to extend the date for filing its next rate case to May 1, 2012. Eagle Rock...

  20. 76 FR 22695 - Yuba City Cogenerations Partners, LP; Supplemental Notice That Initial Market-Based Rate Filing...

    Science.gov (United States)

    2011-04-22

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Yuba City Cogenerations Partners, LP; Supplemental Notice That Initial... notice in the above-referenced proceeding of Yuba City Cogeneration Partners, LP's application for market...

  1. 78 FR 338 - Buckeye Hammond Railroad, L.L.C.; Acquisition and Operation Exemption; Buckeye Partners, L.P.

    Science.gov (United States)

    2013-01-03

    ...] Buckeye Hammond Railroad, L.L.C.; Acquisition and Operation Exemption; Buckeye Partners, L.P. Buckeye Hammond Railroad, L.L.C. (BHRR), a noncarrier, has filed a verified notice of exemption under 49 CFR 1150.31 \\1\\ to acquire from Buckeye Partners, L.P., a noncarrier, and to operate approximately 6,797 feet...

  2. Unge indvandrere på kontanthjælp i Københavns kommune: Barrierer for uddannelse og arbejde

    DEFF Research Database (Denmark)

    Pico Geerdsen, Lars; Jakobsen, Vibeke

    I dette arbejdspapir undersøges, hvilke barrierer for uddannelse og beskæftigelse, der eksisterer for unge indvandrere på kontanthjælp i Københavns Kommune. I undersøgelsen gives en karakteristik af de unge indvandrere på kontanthjælp. Desuden analyseres deres kontakt med aktiveringssystemet og e...

  3. 76 FR 36910 - Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas...

    Science.gov (United States)

    2011-06-23

    ... Energy Regulatory Commission Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas Turbine Power LLC, Dunkirk Power LLC, Huntley Power LLC, Oswego Harbor Power LLC, TC... that on June 15, 2011, Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC...

  4. 76 FR 34692 - Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas...

    Science.gov (United States)

    2011-06-14

    ... Energy Regulatory Commission Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas Turbine Power LLC, Dunkirk Power LLC, Huntley Power LLC, Oswego Harbor Power LLC, TC... (Commission), 18 CFR 385.206 (2011), Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill...

  5. 78 FR 4842 - Linden VFT, LLC v. Brookfield Energy Marketing, LP, Cargill Power Markets, LLC; Notice of Complaint

    Science.gov (United States)

    2013-01-23

    ... Energy Regulatory Commission Linden VFT, LLC v. Brookfield Energy Marketing, LP, Cargill Power Markets... 206 and 306 of the Federal Power Act, 16 U.S.C. 824(e) and 825(e), Linden VFT, LLC (Complainant) filed a formal complaint against Brookfield Energy Marketing, LP and Cargill Power Markets, LLC...

  6. 77 FR 60430 - Brigham Oil & Gas, L.P.; Notice for Temporary Waiver of Filing and Reporting Requirements

    Science.gov (United States)

    2012-10-03

    ... Federal Energy Regulatory Commission Brigham Oil & Gas, L.P.; Notice for Temporary Waiver of Filing and... Rules of Practice and Procedure, 18 CFR 385.204 (2012), Brigham Oil & Gas, L.P., (Brigham Oil & Gas.... Brigham Oil & Gas requested review on an expedited basis and that a Commission order granting the...

  7. Estimating the Population Impact of Lp(a) Lowering on the Incidence of Myocardial Infarction and Aortic Stenosis

    DEFF Research Database (Denmark)

    Afshar, Mehdi; Kamstrup, Pia R; Williams, Ken

    2016-01-01

    OBJECTIVE: High lipoprotein(a) (Lp[a]) is the most common genetic dyslipidemia and is a causal factor for myocardial infarction (MI) and aortic stenosis (AS). We sought to estimate the population impact of Lp(a) lowering that could be achieved in primary prevention using the therapies in developm...

  8. Profiles of organic food consumers in a large sample of French adults: results from the Nutrinet-Santé cohort study.

    Science.gov (United States)

    Kesse-Guyot, Emmanuelle; Péneau, Sandrine; Méjean, Caroline; Szabo de Edelenyi, Fabien; Galan, Pilar; Hercberg, Serge; Lairon, Denis

    2013-01-01

    Lifestyle, dietary patterns and nutritional status of organic food consumers have rarely been described, while interest for a sustainable diet is markedly increasing. Consumer attitude and frequency of use of 18 organic products were assessed in 54,311 adult participants in the Nutrinet-Santé cohort. Cluster analysis was performed to identify behaviors associated with organic product consumption. Socio-demographic characteristics, food consumption and nutrient intake across clusters are provided. Cross-sectional association with overweight/obesity was estimated using polytomous logistic regression. Five clusters were identified: 3 clusters of non-consumers whose reasons differed, occasional (OCOP, 51%) and regular (RCOP, 14%) organic product consumers. RCOP were more highly educated and physically active than other clusters. They also exhibited dietary patterns that included more plant foods and less sweet and alcoholic beverages, processed meat or milk. Their nutrient intake profiles (fatty acids, most minerals and vitamins, fibers) were healthier and they more closely adhered to dietary guidelines. In multivariate models (after accounting for confounders, including level of adherence to nutritional guidelines), compared to those not interested in organic products, RCOP participants showed a markedly lower probability of overweight (excluding obesity) (25 ≤ body mass indexorganic products, a sizeable group in our sample, exhibit specific socio-demographic characteristics, and an overall healthy profile which should be accounted for in further studies analyzing organic food intake and health markers.

  9. Profiles of Organic Food Consumers in a Large Sample of French Adults: Results from the Nutrinet-Santé Cohort Study

    Science.gov (United States)

    Kesse-Guyot, Emmanuelle; Péneau, Sandrine; Méjean, Caroline; Szabo de Edelenyi, Fabien; Galan, Pilar; Hercberg, Serge; Lairon, Denis

    2013-01-01

    Background Lifestyle, dietary patterns and nutritional status of organic food consumers have rarely been described, while interest for a sustainable diet is markedly increasing. Methods Consumer attitude and frequency of use of 18 organic products were assessed in 54,311 adult participants in the Nutrinet-Santé cohort. Cluster analysis was performed to identify behaviors associated with organic product consumption. Socio-demographic characteristics, food consumption and nutrient intake across clusters are provided. Cross-sectional association with overweight/obesity was estimated using polytomous logistic regression. Results Five clusters were identified: 3 clusters of non-consumers whose reasons differed, occasional (OCOP, 51%) and regular (RCOP, 14%) organic product consumers. RCOP were more highly educated and physically active than other clusters. They also exhibited dietary patterns that included more plant foods and less sweet and alcoholic beverages, processed meat or milk. Their nutrient intake profiles (fatty acids, most minerals and vitamins, fibers) were healthier and they more closely adhered to dietary guidelines. In multivariate models (after accounting for confounders, including level of adherence to nutritional guidelines), compared to those not interested in organic products, RCOP participants showed a markedly lower probability of overweight (excluding obesity) (25≤body mass indexorganic products, a sizeable group in our sample, exhibit specific socio-demographic characteristics, and an overall healthy profile which should be accounted for in further studies analyzing organic food intake and health markers. PMID:24204721

  10. 75 FR 76317 - Airworthiness Directives; Gulfstream Aerospace LP (Type Certificate Previously Held by Israel...

    Science.gov (United States)

    2010-12-08

    ... Directives; Gulfstream Aerospace LP (Type Certificate Previously Held by Israel Aircraft Industries, Ltd... Israel (CAAI), which is the aviation authority for Israel, has issued Israeli Airworthiness Directive 28... Israel Aircraft Industries, Ltd.): Docket No. FAA-2010-1164; Directorate Identifier 2010-NM-057-AD...

  11. 75 FR 57844 - Airworthiness Directives; Gulfstream Aerospace LP (Type Certificate Previously Held by Israel...

    Science.gov (United States)

    2010-09-23

    ... Aerospace LP (Type Certificate Previously Held by Israel Aircraft Industries, Ltd.) Model Galaxy and... Held by Israel Aircraft Industries, Ltd.): Amendment 39-16438. Docket No. FAA-2010-0555; Directorate... Certificate previously held by Israel Aircraft Industries, Ltd.) Model Galaxy and Gulfstream 200 airplanes...

  12. 77 FR 32069 - Airworthiness Directives; Gulfstream Aerospace LP (Type Certificate Previously Held by Israel...

    Science.gov (United States)

    2012-05-31

    ... Aerospace LP (Type Certificate Previously Held by Israel Aircraft Industries, Ltd.) Airplanes AGENCY... previously held by Israel Aircraft Industries, Ltd.) Model Galaxy and Gulfstream 200 airplanes. This proposed... receive about this proposed AD. Discussion The Civil Aviation Authority of Israel (CAAI), which is the...

  13. 77 FR 58323 - Airworthiness Directives; Gulfstream Aerospace LP (Type Certificate Previously Held by Israel...

    Science.gov (United States)

    2012-09-20

    ... Aerospace LP (Type Certificate Previously Held by Israel Aircraft Industries, Ltd.) Airplanes AGENCY... Previously Held by Israel Aircraft Industries, Ltd.) Model Gulfstream G150 airplanes. This proposed AD was... Israel (CAAI), which is the aviation authority for Israel, has issued Israeli Airworthiness Directive 01...

  14. 75 FR 36296 - Airworthiness Directives; Gulfstream Aerospace LP (Type Certificate Previously Held by Israel...

    Science.gov (United States)

    2010-06-25

    ... Directives; Gulfstream Aerospace LP (Type Certificate Previously Held by Israel Aircraft Industries, Ltd... The Civil Aviation Authority of Israel (CAAI), which is the aviation authority for Israel, has issued... Previously Held by Israel Aircraft Industries, Ltd.): Docket No. FAA-2010-0555; Directorate Identifier 2010...

  15. 78 FR 47546 - Airworthiness Directives; Gulfstream Aerospace LP (Type Certificate Previously Held by Israel...

    Science.gov (United States)

    2013-08-06

    ... Aerospace LP (Type Certificate Previously Held by Israel Aircraft Industries, Ltd.) Airplanes AGENCY... Certificate Previously Held by Israel Aircraft Industries, Ltd.) Model Gulfstream 100 airplanes, and Model... of Israel (CAAI), which is the aviation authority for Israel, has issued Israeli Airworthiness...

  16. 78 FR 35263 - Freeport LNG Development, L.P.; Application for Blanket Authorization To Export Previously...

    Science.gov (United States)

    2013-06-12

    ... the LNG at the time of export. The Application was filed under section 3 of the Natural Gas Act (NGA... not prohibited by U.S. law or policy. Current Application The current Application is filed in... Freeport LNG Development, L.P.; Application for Blanket Authorization To Export Previously Imported...

  17. State of the Art in LP-WAN Solutions for Industrial IoT Services

    Directory of Open Access Journals (Sweden)

    Ramon Sanchez-Iborra

    2016-05-01

    Full Text Available The emergence of low-cost connected devices is enabling a new wave of sensorization services. These services can be highly leveraged in industrial applications. However, the technologies employed so far for managing this kind of system do not fully cover the strict requirements of industrial networks, especially those regarding energy efficiency. In this article a novel paradigm, called Low-Power Wide Area Networking (LP-WAN, is explored. By means of a cellular-type architecture, LP-WAN–based solutions aim at fulfilling the reliability and efficiency challenges posed by long-term industrial networks. Thus, the most prominent LP-WAN solutions are reviewed, identifying and discussing the pros and cons of each of them. The focus is also on examining the current deployment state of these platforms in Spain. Although LP-WAN systems are at early stages of development, they represent a promising alternative for boosting future industrial IIoT (Industrial Internet of Things networks and services.

  18. 76 FR 57087 - Stone Harbor Investment Partners LP, et al.; Notice of Application

    Science.gov (United States)

    2011-09-15

    ..., or cause more than 10% of the acquired company's voting stock to be owned by investment companies and... COMMISSION Stone Harbor Investment Partners LP, et al.; Notice of Application September 7, 2011. AGENCY... the Investment Company Act of 1940 (``Act'') for an exemption from rule 12d1-2(a) under the Act...

  19. 77 FR 71585 - Brookfield Energy Marketing LP v. ISO New England Inc.; Notice of Complaint

    Science.gov (United States)

    2012-12-03

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Brookfield Energy Marketing LP v. ISO New England Inc.; Notice of... Procedure of the Federal Energy Regulatory Commission (Commission), 18 CFR 385.206 and sections 206 and 306...

  20. 76 FR 66618 - Airworthiness Directives; Eurocopter France (Eurocopter) Model EC225LP Helicopters

    Science.gov (United States)

    2011-10-27

    ...-43-AD; Amendment 39-16815; AD 2011-20-05] RIN 2120-AA64 Airworthiness Directives; Eurocopter France (Eurocopter) Model EC225LP Helicopters AGENCY: Federal Aviation Administration, DOT. ACTION: Final rule... directive to read as follows: 2011-20-05 Eurocopter France (Eurocopter): Amendment 39-16815; Docket No. FAA...

  1. 76 FR 66613 - Airworthiness Directives; Eurocopter France (Eurocopter) Model EC225LP Helicopters

    Science.gov (United States)

    2011-10-27

    ...-028-AD; Amendment 39-16834; AD 2011-21-11] RIN 2120-AA64 Airworthiness Directives; Eurocopter France (Eurocopter) Model EC225LP Helicopters AGENCY: Federal Aviation Administration, DOT. ACTION: Final rule... amended by adding a new airworthiness directive (AD) to read as follows: 2011-21-11 Eurocopter France...

  2. A novel cold-adapted lipase, LP28, from a mesophilic Streptomyces strain.

    Science.gov (United States)

    Simkhada, Jaya Ram; Yoo, Hah Young; Cho, Seung Sik; Choi, Yun Hee; Kim, Seung Wook; Park, Don Hee; Yoo, Jin Cheol

    2012-01-01

    Fossil fuel is limited but its usage has been growing rapidly, thus the fuel is predicted to be completely running out and causing an unbearable global energy crisis in the near future. To solve this potential crisis, incorporating with increasing environmental concerns, significant attentions have been given to biofuel production in the recent years. With the aim of isolating a microbial biocatalyst with potential application in the field of biofuel, a lipase from Streptomyces sp. CS628, LP28, was purified using hydroxyapatite column chromatography followed by a gel filtration. Molecular weight of LP28 was estimated to be 32,400 Da by SDS-PAGE. The activity was the highest at 30 °C and pH 8.0 and was stable at pH 6.0-8.0 and below 25 °C. The enzyme preferentially hydrolyzed p-nitrophenyl decanoate (C10), a medium chain substrate. Furthermore, LP28 non-specifically hydrolyzed triolein releasing both 1,2- and 1,3-diolein. More importantly, LP28 manifestly catalyzed biodiesel production using palm oil and methanol; therefore, it can be a potential candidate in the field of biofuel.

  3. Espacios $L_p$ y teoremas de convergencia para integrales vectoriales

    OpenAIRE

    Bravo de la Parra, Rafael

    1987-01-01

    This paper deals with the study of a bilinear integral, which is an extension of Bartle $^\\ast$-integral for locally convex spaces. Attention is focused on the construction of spaces $L_p$, and theorems of convergence of integrable functions.

  4. 78 FR 12048 - Gulf Shore Energy Partners, LP; Notice of Abbreviated Application for Limited Amendment to...

    Science.gov (United States)

    2013-02-21

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Gulf Shore Energy Partners, LP; Notice of Abbreviated Application for Limited Amendment to Certificate of Public Convenience and Necessity On February 11, 2013, Gulf Shore...

  5. 76 FR 41432 - Airworthiness Directives; Gulfstream Aerospace LP (Type Certificate Previously Held by Israel...

    Science.gov (United States)

    2011-07-14

    ... Aerospace LP (Type Certificate Previously Held by Israel Aircraft Industries, Ltd.) Model Galaxy, Gulfstream... actuator centering spring rod was discovered on a model G100 aircraft during a routine scheduled maintenance inspection. * * * This latent failure of a centering spring rod, if not detected and corrected, in...

  6. Norra LP-mudel ajab Eestis juuri / intervjueerinud Sirje Pärismaa

    Index Scriptorium Estoniae

    2016-01-01

    Teaduspõhise LP-mudeli (Learning environment and pedagogical analysis) - abivahendi koolikultuuri parandamiseks ja süsteemsemaks lähenemiseks tekkinud probleemidele - on kasutusele võtnud 10 kooli ja 3 lasteaeda. Norra hariduseksperdid Janne Stoen ja Hanna Jahnsen vastasid Õpetajate Lehe küsimustele

  7. TRIGONOMETRIC QUASI-GREEDY BASES FOR L-p (T; w)

    DEFF Research Database (Denmark)

    Nielsen, Morten

    2009-01-01

    We give a complete characterization of 2π-periodic weights w for which the usual trigonometric system forms a quasi-greedy basis for Lp(T;w), i.e. bases for which simple thresholding approximants converge in norm. The characterization implies that this can happen only for p=2 and whenever the sys...

  8. 78 FR 14778 - Application to Export Electric Energy; Shell Energy North America (US), L.P.

    Science.gov (United States)

    2013-03-07

    ... applicant have a franchised service area. The electric energy that Shell Energy proposes to export to Mexico... Shell Energy application to export electric energy to ] Mexico should be clearly marked with OE Docket... Application to Export Electric Energy; Shell Energy North America (US), L.P. AGENCY: Office of Electricity...

  9. 47 CFR 74.1204 - Protection of FM broadcast, FM Translator and LP100 stations.

    Science.gov (United States)

    2010-10-01

    ... LP100 stations. 74.1204 Section 74.1204 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED... effective radiated power (ERP) to be used is the maximum ERP of the main radiated lobe in the pertinent... component or the horizontal component of the ERP should be used, whichever is greater in the pertinent...

  10. 78 FR 8611 - WINCO Investment Partnership 2008 L.P. and Winstead PC; Notice of Application

    Science.gov (United States)

    2013-02-06

    ... security or other property to the investment company or knowingly purchasing a security or other property... COMMISSION WINCO Investment Partnership 2008 L.P. and Winstead PC; Notice of Application January 31, 2013... under sections 6(b) and 6(e) of the Investment Company Act of 1940 (the ``Act'') granting an exemption...

  11. Weighted $L^p$ estimates for the elliptic Schrödinger operator

    Directory of Open Access Journals (Sweden)

    Fengping Yao

    2014-07-01

    Full Text Available In this paper we study weighted $L^p$ estimates for the elliptic Schrödinger operator $P=-\\Delta +V(x $ with non-negative potentials $V(x$ on $\\mathbb{R}^n~(n\\geq 3$ which belongs to certain reverse Hölder class.

  12. Robust Granger Analysis in Lp Norm Space for Directed EEG Network Analysis.

    Science.gov (United States)

    Li, Peiyang; Huang, Xiaoye; Li, Fali; Wang, Xurui; Zhou, Weiwei; Liu, Huan; Ma, Teng; Zhang, Tao; Guo, Daqing; Yao, Dezhong; Xu, Peng

    2017-11-01

    Granger analysis (GA) is widely used to construct directed brain networks based on various physiological recordings, such as functional magnetic resonance imaging, and electroencephalogram (EEG). However, in real applications, EEGs are inevitably contaminated by unexpected artifacts that may distort the networks because of the L2 norm structure utilized in GAs when estimating directed links. Compared with the L2 norm, the Lp ( ) norm can compress outlier effects. In this paper, an extended GA is constructed by applying the Lp ( ) norm strategy to estimate robust causalities under outlier conditions, and a feasible iteration procedure is utilized to solve the new GA model. A quantitative evaluation using a predefined simulation network demonstrates smaller bias errors and higher linkage consistence for the Lp ( , 0.8, 0.6, 0.4, 0.2) -GAs compared with both the Lasso- and L2-GAs under various simulated outlier conditions. Applications in resting-state EEGs that contain ocular artifacts also show that the proposed GA can effectively compress the ocular outlier influence and recover the reliable networks. The proposed Lp-GA may be helpful in capturing the reliable network structure when EEGs are contaminated with artifacts in related studies.

  13. Harried LP-gas marketers recount the recent price/supply nightmare

    Energy Technology Data Exchange (ETDEWEB)

    Prowler, S

    1990-02-01

    This article presents a survey of several LP-gas marketers, who discuss how their companies responded to the propane fuel crisis during the winter of 1989-1990. wholesale and retail pricing, as well as consumer response during this period are described. Supply strategies for the future are discussed.

  14. Serum Lp-PLA2: as a novel viewpoint in periodontal treatment of hyperlipidaemics.

    Science.gov (United States)

    Fentoğlu, Özlem; Kirzioğlu, Fatma Yeşim; Tözüm Bulut, Memduha; Kurgan, Şivge; Koçak, Havva; Sütcü, Recep; Kale Köroğlu, Banu; Günhan, Meral

    2015-01-01

    To evaluate the effects of periodontal treatment on serum lipoprotein-associated phospholipase A2 (Lp-PLA2) and C-reactive protein (CRP) levels in hyperlipidaemic patients with periodontitis. The study included 52 hyperlipidaemics and 28 systemically healthy controls (C) with periodontitis. Of the 52 hyperlipidaemics, 29 received a suggested diet (HD), and 23 of them were prescribed statin (HS). Clinical periodontal parameters, serum lipids, Lp-PLA2, and CRP levels were assessed at the baseline and 2 months after the completion of the nonsurgical periodontal treatment (2MPT). Serum parameters were also evaluated 1 week following the periodontal treatment (1WPT). At the baseline, patients in the HS group had a higher percentage of bleeding on probing than those in the C and HD groups. Hyperlipidaemics had higher serum triglyceride levels than the control group at 2MPT compared to the baseline. At 2MPT, the levels of Lp-PLA2 in the HS group were significantly higher compared to the baseline and 1WPT. There were no statistically significant differences in CRP levels between study periods for all groups. The periodontal treatment may affect the inflammatory control of hyperlipidaemic patients with periodontitis via increased Lp-PLA2 levels and severity of the impaired lipid metabolism. These findings may be important regarding the therapeutic strategies for hyperlipidaemics with periodontitis.

  15. 76 FR 65507 - Notice of Petition for Rate Approval; Eagle Ford Midstream, LP

    Science.gov (United States)

    2011-10-21

    ... Energy Regulatory Commission Notice of Petition for Rate Approval; Eagle Ford Midstream, LP Take notice that on October 11, 2011, (Eagle Ford) filed a petition for rate approval pursuant to section 284.123(b.... Eagle Ford states that it is an existing intrastate pipeline, within the meaning of sections 2(16) and...

  16. Måling af friktion ved hjælp af spindelpressen

    DEFF Research Database (Denmark)

    Henningsen, Poul

    Teknisk Manual. Denne rapport gennemgår forsøgsopstillinger på spindelpressen til måling af friktion. Der bliver kort gennemgået hvilket udstyr der skal bruges, og hvordan data opsamles og transducerne kalibreres. Rapporten er en hjælp til opstilling af udstyret. Beskrivelser af testprincipper og...

  17. 76 FR 65717 - Erie Boulevard Hydropower, L.P.; Notice of Availability of Environmental Assessment

    Science.gov (United States)

    2011-10-24

    ... Energy Regulatory Commission Erie Boulevard Hydropower, L.P.; Notice of Availability of Environmental Assessment In accordance with the National Environmental Policy Act of 1969 and the Federal Energy Regulatory... has prepared an Environmental Assessment (EA) for the project. The project does not occupy any federal...

  18. 76 FR 46287 - Erie Boulevard Hydropower, L.P.; Notice of Availability of Environmental Assessment

    Science.gov (United States)

    2011-08-02

    ... Energy Regulatory Commission Erie Boulevard Hydropower, L.P.; Notice of Availability of Environmental Assessment In accordance with the National Environmental Policy Act of 1969 and the Federal Energy Regulatory... environmental assessment (EA). The project is located on the Sacandaga River in Saratoga County, New York. The...

  19. State of the Art in LP-WAN Solutions for Industrial IoT Services.

    Science.gov (United States)

    Sanchez-Iborra, Ramon; Cano, Maria-Dolores

    2016-05-17

    The emergence of low-cost connected devices is enabling a new wave of sensorization services. These services can be highly leveraged in industrial applications. However, the technologies employed so far for managing this kind of system do not fully cover the strict requirements of industrial networks, especially those regarding energy efficiency. In this article a novel paradigm, called Low-Power Wide Area Networking (LP-WAN), is explored. By means of a cellular-type architecture, LP-WAN-based solutions aim at fulfilling the reliability and efficiency challenges posed by long-term industrial networks. Thus, the most prominent LP-WAN solutions are reviewed, identifying and discussing the pros and cons of each of them. The focus is also on examining the current deployment state of these platforms in Spain. Although LP-WAN systems are at early stages of development, they represent a promising alternative for boosting future industrial IIoT (Industrial Internet of Things) networks and services.

  20. Microextraction by Packed Sorbent (MEPS) and Solid-Phase Microextraction (SPME) as Sample Preparation Procedures for the Metabolomic Profiling of Urine

    Science.gov (United States)

    Silva, Catarina; Cavaco, Carina; Perestrelo, Rosa; Pereira, Jorge; Câmara, José S.

    2014-01-01

    For a long time, sample preparation was unrecognized as a critical issue in the analytical methodology, thus limiting the performance that could be achieved. However, the improvement of microextraction techniques, particularly microextraction by packed sorbent (MEPS) and solid-phase microextraction (SPME), completely modified this scenario by introducing unprecedented control over this process. Urine is a biological fluid that is very interesting for metabolomics studies, allowing human health and disease characterization in a minimally invasive form. In this manuscript, we will critically review the most relevant and promising works in this field, highlighting how the metabolomic profiling of urine can be an extremely valuable tool for the early diagnosis of highly prevalent diseases, such as cardiovascular, oncologic and neurodegenerative ones. PMID:24958388

  1. Association of Lp-PLA2 Mass and Aysmptomatic Intracranial and Extracranial Arterial Stenosis in Hypertension Patients.

    Science.gov (United States)

    Wang, Yan; Zhang, Jin; Qian, Yuesheng; Tang, Xiaofeng; Ling, Huawei; Chen, Kemin; Gao, Pingjin; Zhu, Dingliang

    2015-01-01

    Intracranial arterial stenosis (ICAS) is a common cause of ischemic stroke in Asians, whereas whites tend to have more extracranial lesions. Lipoprotein-associated phospholipase A2 (Lp-PLA2) has been associated with ischemic stroke by a large amount of work. However, there are few studies focusing on the relationship of Lp-PLA2 and asymptomatic ICAS or extracranial arterial stenosis (ECAS). Wehereby sought to explore the relationship of Lp-PLA2 and ICAS, ECAS and concurrent stenosis in stroke-free hypertensive patients in Chinese population. All the subjects were evaluated for the presence and severity of ICAS and ECAS through computerized tomographic angiography (CTA) covered the whole brain down to the level of aortic arch. Lp-PLA2 mass was measured by enzyme linked immunoassay. The association of Lp-PLA2 and vascular stenosis was analyzed through multivariate logistic regression. Among 414 participants, 163 (39.4%) had no ICAS or ECAS, 63 (15.2%) had ECAS only, 111 (26.8%) had ICAS only and 77 (18.6%) had concurrent extraintracranial stenosis. Lp-PLA2 mass was significantly associated with isolated ICAS (OR: 2.3; 95% CI: 1.14-4.64), and concurrent stenosis (OR: 3.93; 95% CI: 1.62-9.51), but was not related to isolated ECAS (OR: 1.54; 95% CI: 0.68-3.48). Lp-PLA2 mass was also associated with moderate to severe ICAS no matter how was the ECAS. Moreover, patients with higher Lp-PLA2 mass showed more sever ICAS and had more intracranial arterial lesions. This study revealed the association of Lp-PLA2 mass with ICAS in stroke-free hypertensive patients in Chinese population. The further long-term cohort study was warranted to elucidate the concrete effect of Lp-PLA2 on the asymptomatic ICAS.

  2. The effect of an inhibitor of gut serotonin (LP533401) during the induction of periodontal disease.

    Science.gov (United States)

    Lima, G M G; Corazza, B J M; Moraes, R M; de Oliveira, F E; de Oliveira, L D; Franco, G C N; Perrien, D S; Elefteriou, F; Anbinder, A L

    2016-10-01

    LP533401 is an inhibitor of tryptophan hydroxylase 1, which regulates serotonin production in the gut. Previous work indicates that LP533401 has an anabolic effect in bone. Thus, we hypothesized that inhibition of gut serotonin production may modulate the host response in periodontal disease. In this study, we aimed to analyze the effects of LP533401 in a rat periodontitis model to evaluate the role of gut serotonin in periodontitis pathophysiology. Twenty-four rats were divided into three groups: treated group (T: ligature-induced periodontal disease and LP533401, 25 mg/kg/d) by gavage; ligature group (L: ligature-induced periodontal disease only); and control group (C: without ligature-induced periodontal disease). After 28 d, radiographic alveolar bone support was measured on digital radiographs, and alveolar bone volume fraction, tissue mineral density and trabeculae characteristics were quantified by microcomputed tomography in the right hemi-mandible. Left hemi-mandibles were decalcified and alveolar bone loss, attachment loss and area of collagen in the gingiva were histologically analyzed. Significant difference between the L and C groups was found, confirming that periodontal disease was induced. We observed no difference between the T and L groups regarding alveolar bone destruction and area of collagen. LP533401 (25 mg/kg/d) for 28 d does not prevent bone loss and does not modulate host response in a rat model of induced periodontal disease. © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  3. 77 FR 74544 - Eagle Fund III, L.P., License No. 07/07-0116; Notice Seeking Exemption Under Section 312 of the...

    Science.gov (United States)

    2012-12-14

    ... ADMINISTRATION Eagle Fund III, L.P., License No. 07/07-0116; Notice Seeking Exemption Under Section 312 of the Small Business Investment Act, Conflicts of Interest Notice is hereby given that Eagle Fund III, L.P... interest, of the Small Business Administration Rules and Regulations (13 CFR part 107). Eagle Fund III, L.P...

  4. Transcriptome analysis of the epidermis of the purple quail-like (q-lp) mutant of silkworm, Bombyx mori.

    Science.gov (United States)

    Wang, Pingyang; Qiu, Zhiyong; Xia, Dingguo; Tang, Shunming; Shen, Xingjia; Zhao, Qiaoling

    2017-01-01

    A new purple quail-like (q-lp) mutant found from the plain silkworm strain 932VR has pigment dots on the epidermis similar to the pigment mutant quail (q). In addition, q-lp mutant larvae are inactive, consume little and grow slowly, with a high death rate and other developmental abnormalities. Pigmentation of the silkworm epidermis consists of melanin, ommochrome and pteridine. Silkworm development is regulated by ecdysone and juvenile hormone. In this study, we performed RNA-Seq on the epidermis of the q-lp mutant in the 4th instar during molting, with 932VR serving as the control. The results showed 515 differentially expressed genes, of which 234 were upregulated and 281 downregulated in q-lp. BLASTGO analysis indicated that the downregulated genes mainly encode protein-binding proteins, membrane components, oxidation/reduction enzymes, and proteolytic enzymes, whereas the upregulated genes largely encode cuticle structural constituents, membrane components, transport related proteins, and protein-binding proteins. Quantitative reverse transcription PCR was used to verify the accuracy of the RNA-Seq data, focusing on key genes for biosynthesis of the three pigments and chitin as well as genes encoding cuticular proteins and several related nuclear receptors, which are thought to play key roles in the q-lp mutant. We drew three conclusions based on the results: 1) melanin, ommochrome and pteridine pigments are all increased in the q-lp mutant; 2) more cuticle proteins are expressed in q-lp than in 932VR, and the number of upregulated cuticular genes is significantly greater than downregulated genes; 3) the downstream pathway regulated by ecdysone is blocked in the q-lp mutant. Our research findings lay the foundation for further research on the developmental changes responsible for the q-lp mutant.

  5. LP-search and its use in analysis of the accuracy of control systems with acoustical models

    Science.gov (United States)

    Sergeyev, V. I.; Sobol, I. M.; Statnikov, R. B.; Statnikov, I. N.

    1973-01-01

    The LP-search is proposed as an analog of the Monte Carlo method for finding values in nonlinear statistical systems. It is concluded that: To attain the required accuracy in solution to the problem of control for a statistical system in the LP-search, a considerably smaller number of tests is required than in the Monte Carlo method. The LP-search allows the possibility of multiple repetitions of tests under identical conditions and observability of the output variables of the system.

  6. Differential Gene Expression of TRPM1, the Potential Cause of Congenital Stationary Night Blindness and Coat Spotting Patterns (LP) in the Appaloosa Horse (Equus caballus)

    Science.gov (United States)

    Bellone, Rebecca R.; Brooks, Samantha A.; Sandmeyer, Lynne; Murphy, Barbara A.; Forsyth, George; Archer, Sheila; Bailey, Ernest; Grahn, Bruce

    2008-01-01

    The appaloosa coat spotting pattern in horses is caused by a single incomplete dominant gene (LP). Homozygosity for LP (LP/LP) is directly associated with congenital stationary night blindness (CSNB) in Appaloosa horses. LP maps to a 6-cM region on ECA1. We investigated the relative expression of two functional candidate genes located in this LP candidate region (TRPM1 and OCA2), as well as three other linked loci (TJP1, MTMR10, and OTUD7A) by quantitative real-time RT–PCR. No large differences were found for expression levels of TJP1, MTMR10, OTUD7A, and OCA2. However, TRPM1 (Transient Receptor Potential Cation Channel, Subfamily M, Member 1) expression in the retina of homozygous appaloosa horses was 0.05% the level found in non-appaloosa horses (R = 0.0005). This constitutes a >1800-fold change (FC) decrease in TRPM1 gene expression in the retina (FC = −1870.637, P = 0.001) of CSNB-affected (LP/LP) horses. TRPM1 was also downregulated in LP/LP pigmented skin (R = 0.005, FC = −193.963, P = 0.001) and in LP/LP unpigmented skin (R = 0.003, FC = −288.686, P = 0.001) and was downregulated to a lesser extent in LP/lp unpigmented skin (R = 0.027, FC = −36.583, P = 0.001). TRP proteins are thought to have a role in controlling intracellular Ca2+ concentration. Decreased expression of TRPM1 in the eye and the skin may alter bipolar cell signaling as well as melanocyte function, thus causing both CSNB and LP in horses. PMID:18660533

  7. Efficacy and safety of the probiotic Lactobacillus paracasei LP-33 in allergic rhinitis

    DEFF Research Database (Denmark)

    Costa, D J; Marteau, P; Amouyal, M

    2014-01-01

    BACKGROUND/OBJECTIVES: An imbalance between Th1 and Th2 cells is involved in allergic rhinitis (AR) that may be improved by probiotics. To test the efficacy of the probiotic Lactobacillus paracasei subsp. paracasei LP-33, a double-blind, placebo-controlled, randomized trial was carried out......). All received loratadine for 5 weeks. The primary end point was the improvement in Rhinitis Quality of Life (RQLQ) global score at the fifth week of LP-33 consumption compared with placebo (in addition to loratadine). Secondary end points included nasal and ocular symptoms (individual and total symptom......=-0.286 (95% confidence interval (CI): -0.536; -0.035)). No significant differences were noted for the change of the rhinitis total symptom score 5 global score between groups (P=0.1288, difference=-0.452 (95% CI: -1.036; 0.132)). Significant differences in ocular symptoms (RQLQ) were observed between...

  8. Switchable narrow linewidth fiber laser with LP11 transverse mode output

    Science.gov (United States)

    Shen, Ya; Ren, Guobin; Yang, Yuguang; Yao, Shuzhi; Wu, Yue; Jiang, Youchao; Xu, Yao; Jin, Wenxing; Zhu, Bofeng; Jian, Shuisheng

    2018-01-01

    We experimentally demonstrate a switchable narrow linewidth single-longitudinal-mode (SLM) erbium-doped fiber (EDF) ring laser with LP11 transverse mode output. The laser is based on a mode selective all-fiber fused coupler which is composed of a single-mode fiber (SMF) and a two-mode fiber (TMF). By controlling the polarization state of the output light, the laser can provide narrow linewidth SLM output with LP11 transverse mode at two specific wavelengths, which correspond to two transmission peaks of the chirped moiré fiber grating (CMFBG). The 20 dB linewidth of the fiber laser for each wavelength is approximately 7.2 and 6.4 kHz.

  9. 16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice.

    Science.gov (United States)

    Walker, Alan W; Martin, Jennifer C; Scott, Paul; Parkhill, Julian; Flint, Harry J; Scott, Karen P

    2015-01-01

    Characterisation of the bacterial composition of the gut microbiota is increasingly carried out with a view to establish the role of different bacterial species in causation or prevention of disease. It is thus essential that the methods used to determine the microbial composition are robust. Here, several widely used molecular techniques were compared to establish the optimal methods to assess the bacterial composition in faecal samples from babies, before weaning. The bacterial community profile detected in the faeces of infants is highly dependent on the methodology used. Bifidobacteria were the most abundant bacteria detected at 6 weeks in faeces from two initially breast-fed babies using fluorescent in situ hybridisation (FISH), in agreement with data from previous culture-based studies. Using the 16S rRNA gene sequencing approach, however, we found that the detection of bifidobacteria in particular crucially depended on the optimisation of the DNA extraction method, and the choice of primers used to amplify the V1-V3 regions of 16S rRNA genes prior to subsequent sequence analysis. Bifidobacteria were only well represented among amplified 16S rRNA gene sequences when mechanical disruption (bead-beating) procedures for DNA extraction were employed together with optimised "universal" PCR primers. These primers incorporate degenerate bases at positions where mismatches to bifidobacteria and other bacterial taxa occur. The use of a DNA extraction kit with no bead-beating step resulted in a complete absence of bifidobacteria in the sequence data, even when using the optimised primers. This work emphasises the importance of sample processing methodology to downstream sequencing results and illustrates the value of employing multiple approaches for determining microbiota composition.

  10. Physical profiling and IR spectroscopy: simple and effective methods to discriminate between genuine and counterfeit samples of Viagra® and Cialis®.

    Science.gov (United States)

    Custers, Deborah; Vandemoortele, Suzanne; Bothy, Jean-Luc; De Beer, Jacques O; Courselle, Patricia; Apers, Sandra; Deconinck, Eric

    2016-01-01

    Counterfeit medicines are a global threat to public health. High amounts enter the European market, enforcing the need for simple techniques to help customs detect these pharmaceuticals. This study focused on physical profiling and IR spectroscopy to obtain a prime discrimination between genuine and illegal Viagra® and Cialis® medicines. Five post-tableting characteristics were explored: colour, mass, long length, short length, and thickness. Hypothesis testing showed that most illegal samples (between 60 and 100%) significantly differ from the genuine medicines, in particular for mass and long length. Classification and Regression Trees (CART) analysis resulted in a good discrimination between genuine and illegal medicines (98.93% correct classification rate for Viagra®, 99.42% for Cialis®). Moreover, CART confirmed the observation that mass and long length are the key physical characteristics which determine the observed discrimination. IR analysis was performed on tablets without blister and on tablets in intact blister. These data were analyzed using Soft Independent Modelling of Class Analogy (SIMCA) and Partial Least Squares - Discriminant Analysis (PLS-DA). Supervised techniques needed to be applied since Principal Component Analysis (PCA) was not able to generate the desired discrimination. Our study shows that a perfect discrimination between genuine and illegal medicines can be made by both SIMCA and PLS-DA without removing the tablets from the blister. This approach has the advantage of keeping the blister intact. Our study demonstrates that these user friendly techniques are reliable methods to aid customs to obtain a prime distinction between genuine and illegal samples on the spot. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.

  11. German vegan study: diet, life-style factors, and cardiovascular risk profile.

    Science.gov (United States)

    Waldmann, A; Koschizke, J W; Leitzmann, C; Hahn, A

    2005-01-01

    Evaluation of cardiovascular risk profile in 154 German vegans. Cross-sectional study, Germany. Study instruments: 2 FFQ, 2 questionnaires, analyses of fasting venous blood samples. The total study population had a low BMI (mean: 22.3 kg/m(2)), a moderate blood pressure (mean: 120/75 mm Hg), an extremely low consumption of alcohol (mean: 0.77 g/day) and 96.8% were nonsmokers. Moderate physical activity (PAL) was reported by nearly 50%, whereas 22.7% declared to have a high PAL (>3 h/week). Median triacylglycerol (TG) was 0.81 mmol/l, total cholesterol (TC) was 4.33 mmol/l, HDL was 1.34 mmol/l. The mean TC/HDL-ratio was 3.3. Lipoprotein(a) (Lp(a)) was 8.13 mg/dl, concentrations of >30 mg/dl were prevalent in 25% of the participants. In general, status of folate and pyridoxine were sufficient, while 49.7% showed cobalamin concentrations vegan nutrition were the main determinants of homocysteine in the total study population. Although TC and LDL concentrations were favorable, low HDL and elevated homocysteine and Lp(a) concentrations were unfavorable. Overall, these results confirm the notion that a vegan diet is deficient in vitamin B(12), which may have an unfavorable effect on CHD risk.

  12. Théorie $L^p$ pour l'équation de Cauchy-Riemann

    OpenAIRE

    Laurent-Thiébaut, Christine

    2013-01-01

    In this paper we propose a systematic study of the Cauchy-Riemann operator in the $L^p$-setting in complex manifolds. We first consider $L^p_{loc}$-theory and then we develop an $L^p$ Andreotti-Grauert theory. Finally we consider Serre duality and its applications to the solvability of the Cauchy-Riemann equation with exact support in $L^p$-spaces.

  13. Th\\'eorie $L^p$ pour l'\\'equation de Cauchy-Riemann

    OpenAIRE

    Laurent-Thiébaut, Christine

    2013-01-01

    In this paper we propose a systematic study of the Cauchy-Riemann operator in the $L^p$-setting in complex manifolds. We first consider $L^p_{loc}$-theory and then we develop an $L^p$ Andreotti-Grauert theory. Finally we consider Serre duality and its applications to the solvability of the Cauchy-Riemann equation with exact support in $L^p$-spaces.

  14. Characteristics of a Monoacylglycerol Lipase Isolated from Pseudomonas sp. LP7315 -Hydrolysis and Synthesis of Monoglycerides

    OpenAIRE

    Sakiyama, Takaharu; Yoshimi, Tsuyoshi; Miyake, Akira; Umeoka, Midori; Tanaka, Atsushi; Ozaki, Sho; Nakanishi, Kazuhiro

    2001-01-01

    A monoacylglycerol lipase (MGL) was purified from Pseudomonas sp. LP7315 by ammonium sulfate precipitation, anion-exchange chromatography, and preparative electrophoresis. The purified enzyme was homogeneous on an SDS-polyacrylamide gel with a molecular mass of 59 kDa. Itshydrolytic activity was confirmed to be specific for monoglycerides: the enzyme did not hydrolyze diandtriglycerides. MGL was found to be stable even after l-h incubation at 65℃. The hydrolytic activity depended not only on ...

  15. A convolution type characterization for Lp - multipliers for the Heisenberg group

    Directory of Open Access Journals (Sweden)

    R. Radha

    2007-01-01

    Full Text Available It is well known that if m is an Lp - multiplier for the Fourier transform on ℝn(1

  16. Safety analysis report -- Packages LP-50 tritium package (Packaging of fissile and other radioactive materials)

    Energy Technology Data Exchange (ETDEWEB)

    Gates, A.A.; McCarthy, P.G.; Edl, J.W.; Chalfant, G.G. (comps.); Cadelli, G.

    1975-05-01

    Elemental tritium is shipped at low pressure in a stainless steel container (LP-50) surrounded by an aluminum vessel and Celotex insulation at least 4 in. thick in a steel drum. Each package contains a large quantity (greater than a Type A quantity) of nonfissile material, as defined in AECM 0529. This report provides the details of the safety analysis performed for this type container.

  17. A profile of US-Mexico border mobility among a stratified random sample of Hispanics living in the El Paso-Juarez area.

    Science.gov (United States)

    Lapeyrouse, L M; Morera, O; Heyman, J M C; Amaya, M A; Pingitore, N E; Balcazar, H

    2012-04-01

    Examination of border-specific characteristics such as trans-border mobility and transborder health service illuminates the heterogeneity of border Hispanics and may provide greater insight toward understanding differential health behaviors and status among these populations. In this study, we create a descriptive profile of the concept of trans-border mobility by exploring the relationship between mobility status and a series of demographic, economic and socio-cultural characteristics among mobile and non-mobile Hispanics living in the El Paso-Juarez border region. Using a two-stage stratified random sampling design, bilingual interviewers collected survey data from border residents (n = 1,002). Findings show that significant economic, cultural, and behavioral differences exist between mobile and non-mobile respondents. While non-mobile respondents were found to have higher social economic status than their mobile counterparts, mobility across the border was found to offer less acculturated and poorer Hispanics access to alternative sources of health care and other services.

  18. Calling the lp_solve Linear Program Software from R, S-PLUS and Excel

    Directory of Open Access Journals (Sweden)

    Samuel E. Buttrey

    2005-05-01

    Full Text Available We present a link that allows R, S-PLUS and Excel to call the functions in the lp_solve system. lp_solve is free software (licensed under the GNU Lesser GPL that solves linear and mixed integer linear programs of moderate size (on the order of 10,000 variables and 50,000 constraints. R does not include this ability (though two add-on packages offer linear programs without integer variables, while S-PLUS users need to pay extra for the NuOPT library in order to solve these problems. Our link manages the interface between these statistical packages and lp_solve. Excel has a built-in add-in named Solver that is capable of solving mixed integer programs, but only with fewer than 200 variables. This link allows Excel users to handle substantially larger problems at no extra cost. While our primary concern has been the Windows operating system, the package has been tested on some Unix-type systems as well.

  19. The Leptospiral Antigen Lp49 is a Two-Domain Protein with Putative Protein Binding Function

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira Giuseppe,P.; Oliveira Neves, F.; Nascimento, A.; Gomes Guimaraes, B.

    2008-01-01

    Pathogenic Leptospira is the etiological agent of leptospirosis, a life-threatening disease that affects populations worldwide. Currently available vaccines have limited effectiveness and therapeutic interventions are complicated by the difficulty in making an early diagnosis of leptospirosis. The genome of Leptospira interrogans was recently sequenced and comparative genomic analysis contributed to the identification of surface antigens, potential candidates for development of new vaccines and serodiagnosis. Lp49 is a membrane-associated protein recognized by antibodies present in sera from early and convalescent phases of leptospirosis patients. Its crystal structure was determined by single-wavelength anomalous diffraction using selenomethionine-labelled crystals and refined at 2.0 Angstroms resolution. Lp49 is composed of two domains and belongs to the all-beta-proteins class. The N-terminal domain folds in an immunoglobulin-like beta-sandwich structure, whereas the C-terminal domain presents a seven-bladed beta-propeller fold. Structural analysis of Lp49 indicates putative protein-protein binding sites, suggesting a role in Leptospira-host interaction. This is the first crystal structure of a leptospiral antigen described to date.

  20. The First Photometric Investigation of the Neglected Contact Binary LP Comae

    Science.gov (United States)

    Liu, Jinzhong; Zhang, Yu; Song, Fangfang; Yang, Taozhi

    2017-10-01

    In this paper, we present the first complete photometric light curves in the B, V, and R passbands of the neglected contact binary LP Com. Based on the Wilson-Devinney code, it has been found that the LP Com is a W-type W UMa binary with a mass ratio of q={m}2/{m}1=4.36 and a contact degree of f = 21.3 ± 0.3%. By using our new times of minimum light together with those collected from the literature, we found that the observed calculated (O-C) curve shows an upward parabolic variation that corresponds to a long-term increase in the orbital period at a rate of {dP}/{dt}=+2.59× {10}-7 days yr-1. Such a long-term increase in its orbital period may be caused by a mass transfer from the less massive component to the more massive one. This indicates that LP Com is in the thermal relaxation oscillation controlled evolution stage. The presence of two dark spots on both companions was used to fit the asymmetry of the light curves. When the upward parabolic change was subtracted, the {(O-C)}2 curve reveals a cyclic variation with a period of 10.09 yr and a small amplitude of 0.0028 days. The cyclic variation was investigated by the light-time effect due to the presence of an extremely cool stellar companion.

  1. Stress Corrosion Behavior of 12Cr Martensite Steel for Steam Turbine LP Blade

    Science.gov (United States)

    Tianjian, Wang; Yubing, Pei; Zhenhuan, Gao; Hua, Fan; Gongxian, Yang

    With the development of capacity and efficiency of coal-fired thermal power plant, the length of Low Pressure (LP) last-stage blade of steam turbine became longer. Therefore, the design static stress of blade gets closer or even higher than the yield strength of material. Because of the special operation condition of LP last stage blade, the stress corrosion crack of 12Cr-Ni-Mo-V-N Martensite stainless steel may happen especially at the root of the blade where designed the highest static stress. In this paper, the stress corrosion behavior of 12Cr-Ni-Mo-V-N Martensite stainless steels used for steam turbine LP last stage blade in 3vol% NaCl solution was studied, the constant stress is about 95%, 85%, 65% and 35% of yield stress respectively and the test was lasted for 3000 hours, the stress corrosion behavior was studied and then, the effect of shot penning strengthen for anti-stress corrosion property of 12Cr-Ni-Mo-V-N Martensitic steel was studied. The results showed that the purity of steel affects the stress corrosion behavior huge especially at the high and medium stress condition. The shot penning cannot enhances the anti-stress corrosion property of the 12Cr-Ni-Mo-V-N steel at high tensile constant stress condition, however it will make the anti-stress corrosion property better when the stress is low.

  2. Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX.

    Science.gov (United States)

    Lu, Wei; Zhou, Qingzhang; Yang, Hao; Wang, Hao; Gu, Yexing; Shen, Qi; Xue, Jinglun; Dong, Xiaoyan; Chen, Jinzhong

    2016-06-01

    Hemophilia B is a hemorrhagic disease caused by the deficiency of clotting factor IX (FIX). Gene therapy might be the ultimate strategy for the disease. However, two main problems that should be solved in gene therapy for hemophilia B are immunity and safety. Self-complementary adeno-associated virus serotype 8 (scAAV8), a non-human primate AAV featuring low immunogenicity and high transfection efficiency in liver cells, might be a potential vector for hemophilia B gene therapy. A strong liver-specific promoter-1 (LP1) was inserted and mutant human FIX Arg338Ala was introduced into plasmid scAAV8-LP1 to develop an optimized AAV8 vector that expresses human clotting factor FIX (hFIX). The efficiency of scAAV8-LP1-hFIX administered through normal systemic injection or hydrodynamic injection was compared. A high expression was achieved using hydrodynamic injection, and the peak hFIX expression levels in the 5 × 10(11) and 1 × 10(11) virus genome (vg) cohorts were 31.94% and 25.02% of normal level, respectively, at 60 days post-injection. From the perspective of long-term (200 days) expression, both injection methods presented promising results with the concentration value maintained above 4% of normal plasma. The results were further verified by enzyme-linked immunosorbent assay and activated partial thromboplastin time. Our study provides a potential gene therapy method for hemophilia B.

  3. 77 FR 21587 - Bayer Cropscience, LP, Including On-Site Leased Workers From Jacobs PSG, Middough Associates, Inc...

    Science.gov (United States)

    2012-04-10

    ... Employment and Training Administration Bayer Cropscience, LP, Including On-Site Leased Workers From Jacobs PSG, Middough Associates, Inc., Adecco, CDI Engineering Solutions, Becht Engineering, Engineering... Engineering Solutions, Becht Engineering, Engineering Support Systems, Manufacturing Management Services, US...

  4. Remarks on the Phragmen-Lindelof theorem for Lp-viscosity solutions of fully nonlinear PDEs with unbounded ingredients

    Directory of Open Access Journals (Sweden)

    Kazushige Nakagawa

    2009-11-01

    Full Text Available The Phragmen-Lindelof theorem for Lp-viscosity solutions of fully nonlinear second order elliptic partial differential equations with unbounded coefficients and inhomogeneous terms is established.

  5. Lq -Lp optimization for multigrid fluorescence tomography of small animals using simplified spherical harmonics

    Science.gov (United States)

    Edjlali, Ehsan; Bérubé-Lauzière, Yves

    2018-01-01

    We present the first Lq -Lp optimization scheme for fluorescence tomographic imaging. This is then applied to small animal imaging. Fluorescence tomography is an ill-posed, and in full generality, a nonlinear problem that seeks to image the 3D concentration distribution of a fluorescent agent inside a biological tissue. Standard candidates for regularization to deal with the ill-posedness of the image reconstruction problem include L1 and L2 regularization. In this work, a general Lq -Lp regularization framework (Lq discrepancy function - Lp regularization term) is introduced for fluorescence tomographic imaging. A method to calculate the gradient for this general framework is developed which allows evaluating the performance of different cost functions/regularization schemes in solving the fluorescence tomographic problem. The simplified spherical harmonics approximation is used to accurately model light propagation inside the tissue. Furthermore, a multigrid mesh is utilized to decrease the dimension of the inverse problem and reduce the computational cost of the solution. The inverse problem is solved iteratively using an lm-BFGS quasi-Newton optimization method. The simulations are performed under different scenarios of noisy measurements. These are carried out on the Digimouse numerical mouse model with the kidney being the target organ. The evaluation of the reconstructed images is performed both qualitatively and quantitatively using several metrics including QR, RMSE, CNR, and TVE under rigorous conditions. The best reconstruction results under different scenarios are obtained with an L1.5 -L1 scheme with premature termination of the optimization process. This is in contrast to approaches commonly found in the literature relying on L2 -L2 schemes.

  6. Laser polarimeter LP101M and its applications in liquid cromatography

    Science.gov (United States)

    Fajer, V.; Rodriguez, C.; Gonzalez, R.; Cossio, G.; Martinez, M.; Bravo, O.

    1996-02-01

    A high sensitivity laser polarimeter LP101M employing a He-Ne laser has been designed and constructed as a detector for liquid chromatography achieving a sensitivity better than 0.001 degree. The operation principle and technical characteristics of this instrument are described. A liquid gel chromatography column system suitable for sugar cane juice analysis was also designed and calibrated. It separated and analyzed the medium molecular weight carbohydrates and demonstrated the strong influence of these substances in the conventional polarimetric determinations.

  7. Nonlinear optics in the LP(02) higher-order mode of a fiber.

    Science.gov (United States)

    Chen, Y; Chen, Z; Wadsworth, W J; Birks, T A

    2013-07-29

    The distinct disperion properties of higher-order modes in optical fibers permit the nonlinear generation of radiation deeper into the ultraviolet than is possible with the fundamental mode. This is exploited using adiabatic, broadband mode convertors to couple light efficiently from an input fundamental mode and also to return the generated light to an output fundamental mode over a broad spectral range. For example, we generate visible and UV supercontinuum light in the LP(02) mode of a photonic crystal fiber from sub-ns pulses with a wavelength of 532 nm.

  8. Fault-Tolerant Facility Location: A Randomized Dependent LP-Rounding Algorithm

    Science.gov (United States)

    Byrka, Jaroslaw; Srinivasan, Aravind; Swamy, Chaitanya

    We give a new randomized LP-rounding 1.725-approximation algorithm for the metric Fault-Tolerant Uncapacitated Facility Location problem. This improves on the previously best known 2.076-approximation algorithm of Swamy & Shmoys. To the best of our knowledge, our work provides the first application of a dependent-rounding technique in the domain of facility location. The analysis of our algorithm benefits from, and extends, methods developed for Uncapacitated Facility Location; it also helps uncover new properties of the dependent-rounding approach.

  9. Antioxidant and inflammatory aspects of lipoprotein-associated phospholipase A2 (Lp-PLA2 : a review

    Directory of Open Access Journals (Sweden)

    Damasceno Nágila RT

    2011-09-01

    Full Text Available Abstract The association of cardiovascular events with Lp-PLA2 has been studied continuously today. The enzyme has been strongly associated with several cardiovascular risk markers and events. Its discovery was directly related to the hydrolysis of the platelet-activating factor and oxidized phospholipids, which are considered protective functions. However, the hydrolysis of bioactive lipids generates lysophospholipids, compounds that have a pro-inflammatory function. Therefore, the evaluation of the distribution of Lp-PLA2 in the lipid fractions emphasized the dual role of the enzyme in the inflammatory process, since the HDL-Lp-PLA2 enzyme contributes to the reduction of atherosclerosis, while LDL-Lp-PLA2 stimulates this process. Recently, it has been verified that diet components and drugs can influence the enzyme activity and concentration. Thus, the effects of these treatments on Lp-PLA2 may represent a new kind of prevention of cardiovascular disease. Therefore, the association of the enzyme with the traditional assessment of cardiovascular risk may help to predict more accurately these diseases.

  10. The Lp Lp Lp-curvature images of convex bodies and Lp Lp Lp ...

    Indian Academy of Sciences (India)

    Associated with the -curvature image defined by Lutwak, some inequalities for extended mixed -affine surface areas of convex bodies and the support functions of -projection bodies are established. As a natural extension of a result due to Lutwak, an -type affine isoperimetric inequality, whose special cases are ...

  11. Effect of nicotine on melanogenesis and antioxidant status in HEMn-LP melanocytes

    Energy Technology Data Exchange (ETDEWEB)

    Delijewski, Marcin; Beberok, Artur; Otręba, Michał; Wrześniok, Dorota; Rok, Jakub; Buszman, Ewa, E-mail: ebuszman@sum.edu.pl

    2014-10-15

    Nicotine is a natural ingredient of tobacco plants and is responsible for the addictive properties of tobacco. Nowadays nicotine is also commonly used as a form of smoking cessation therapy. It is suggested that nicotine may be accumulated in human tissues containing melanin. This may in turn affect biochemical processes in human cells producing melanin. The aim of this study was to examine the effect of nicotine on melanogenesis and antioxidant status in cultured normal human melanocytes HEMn-LP. Nicotine induced concentration-dependent loss in melanocytes viability. The value of EC{sub 50} was determined to be 7.43 mM. Nicotine inhibited a melanization process in human light pigmented melanocytes and caused alterations of antioxidant defense system. Significant changes in cellular antioxidant enzymes: superoxide dismutase and catalase activities and in hydrogen peroxide content were stated. The obtained results may explain a potential influence of nicotine on biochemical processes in melanocytes in vivo during long term exposition to nicotine. - Graphical abstract: Nicotine inhibits melanogenesis and induces oxidative stress in HEMn-LP melanocytes. - Highlights: • Nicotine induces concentration-dependent loss in melanocytes viability. • Nicotine in non-cytotoxic concentrations inhibits melanogenesis. • Nicotine in higher concentrations induces oxidative stress.

  12. Observerless Scheme for Sensorless Speed Control of PMSM Using Direct Torque Control Method With LP Filter

    Directory of Open Access Journals (Sweden)

    BEKIROGLU, N.

    2010-08-01

    Full Text Available In this study, direct torque control (DTC of a permanent magnet synchronous motor is realized with a sensorless speed control technique without using an observer. Space vector pulse width modulation (SVPWM technique is applied in order to determine the switching sequence of the voltage source inverter. Torque and flux, the main variables of the DTC, are estimated by using the mathematical model of the motor. Estimated torque and flux values are compared with their references in every control cycle. Then, according to the torque and flux demand, the voltage vector is constituted. In the proposed control scheme, speed is estimated by using flux calculations and a PI controller is used to process the torque and flux errors. Furthermore, a low-pass (LP filter is implemented within the proposed system for voltage and current harmonics suppression. The results proved that proposed scheme for the DTC provides the speed control under various torque demands without employing a sensor. The proposed system performs very well for a sensorless operation and effectively eliminates the harmonics due to the LP filter.

  13. Immune-enhancing effect of nanometricLactobacillus plantarumnF1 (nLp-nF1) in a mouse model of cyclophosphamide-induced immunosuppression.

    Science.gov (United States)

    Choi, Dae-Woon; Jung, Sun Young; Kang, Jisu; Nam, Young-Do; Lim, Seong-Il; Kim, Ki Tae; Shin, Hee Soon

    2017-11-15

    Nanometric Lactobacillus plantarum nF1 (nLp-nF1) is a biogenics consisting of dead L. plantarum pre-treated with heat and a nanodispersion process. In this study, we investigated the immune-enhancing effects of nLp-nF1 in vivo and in vitro . To evaluate the immunostimulatory effects of nLp-nF1, mice immunosuppressed by cyclophosphamide (CPP) treatment were administered with nLp-nF1. As expected, CPP restricted the immune response of mice, whereas oral administration of nLp-nF1 significantly increased total IgG in serum, and cytokine production [interleukin-12 (IL-12) and tumor necrosis factor alpha (TNF-α)] in bone marrow cells. Furthermore, nLp-nF1 enhanced the production of splenic cytokines such as IL-12, TNF-α, and interferon gamma (IFN-γ). In vitro , nLp-nF1 stimulated the immune response by enhancing the production of cytokines such as IL-12, TNF-α, and IFN-γ. Moreover, nLp-nF1 given a food additive enhanced the immune responses when combined with various food materials on in vitro . These results suggest that nLp-nF1 could be used to strengthen the immune system and recover normal immunity in people with a weaker immune system, such as children, the elderly, and patients.

  14. Serial CSF sampling over a period of 30 h via an indwelling spinal catheter in healthy volunteers : headache, back pain, tolerability and measured acetylcholine profile

    NARCIS (Netherlands)

    den Daas, Izaak; Wemer, Johan; Abou Farha, Khalid; Tamminga, Wim; de Boer, Theo; Spanjersberg, Rob; Struys, Michel M. R. F.; Absalom, Anthony R.

    Timed interval cerebrospinal fluid (CSF) sampling by indwelling catheterization can be a valuable corroborative tool for the pharmacokinetic and pharmacodynamic assessment of drugs. CSF sampling in studies on drug candidates for Alzheimer's disease have been conducted in evaluations of the

  15. Expression Profiling Smackdown: Human Transcriptome Array HTA 2.0 vs. RNA-Seq

    Science.gov (United States)

    Palermo, Meghann; Driscoll, Heather; Tighe, Scott; Dragon, Julie; Bond, Jeff; Shukla, Arti; Vangala, Mahesh; Vincent, James; Hunter, Tim

    2014-01-01

    The advent of both microarray and massively parallel sequencing have revolutionized high-throughput analysis of the human transcriptome. Due to limitations in microarray technology, detecting and quantifying coding transcript isoforms, in addition to non-coding transcripts, has been challenging. As a result, RNA-Seq has been the preferred method for characterizing the full human transcriptome, until now. A new high-resolution array from Affymetrix, GeneChip Human Transcriptome Array 2.0 (HTA 2.0), has been designed to interrogate all transcript isoforms in the human transcriptome with >6 million probes targeting coding transcripts, exon-exon splice junctions, and non-coding transcripts. Here we compare expression results from GeneChip HTA 2.0 and RNA-Seq data using identical RNA extractions from three samples each of healthy human mesothelial cells in culture, LP9-C1, and healthy mesothelial cells treated with asbestos, LP9-A1. For GeneChip HTA 2.0 sample preparation, we chose to compare two target preparation methods, NuGEN Ovation Pico WTA V2 with the Encore Biotin Module versus Affymetrix's GeneChip WT PLUS with the WT Terminal Labeling Kit, on identical RNA extractions from both untreated and treated samples. These same RNA extractions were used for the RNA-Seq library preparation. All analyses were performed in Partek Genomics Suite 6.6. Expression profiles for control and asbestos-treated mesothelial cells prepared with NuGEN versus Affymetrix target preparation methods (GeneChip HTA 2.0) are compared to each other as well as to RNA-Seq results.

  16. Cancer associated epigenetic transitions identified by genome-wide histone methylation binding profiles in human colorectal cancer samples and paired normal mucosa

    DEFF Research Database (Denmark)

    Enroth, Stefan; Rada-Iglesisas, Alvaro; Andersson, Robin

    2011-01-01

    Despite their well-established functional roles, histone modifications have received less attention than DNA methylation in the cancer field. In order to evaluate their importance in colorectal cancer (CRC), we generated the first genome-wide histone modification profiles in paired normal colon...

  17. Longitudinal Stability of Social Competence Indicators in a Portuguese Sample: Q-Sort Profiles of Social Competence, Measures of Social Engagement, and Peer Sociometric Acceptance

    Science.gov (United States)

    Santos, António J.; Vaughn, Brian E.; Peceguina, Inês; Daniel, João R.

    2014-01-01

    This study examines the temporal stability (over 3 years) of individual differences in 3 domains relevant to preschool children's social competence: social engagement/motivation, profiles of behavior and personality attributes characteristic of socially competent young children, and peer acceptance. Each domain was measured with multiple…

  18. Ultra-processed foods have the worst nutrient profile, yet they are the most available packaged products in a sample of New Zealand supermarkets

    NARCIS (Netherlands)

    Luiten, Claire M; Steenhuis, Ingrid Hm; Eyles, Helen; Ni Mhurchu, Cliona; Waterlander, Wilma E

    OBJECTIVE: To examine the availability of packaged food products in New Zealand supermarkets by level of industrial processing, nutrient profiling score (NPSC), price (energy, unit and serving costs) and brand variety. DESIGN: Secondary analysis of cross-sectional survey data on packaged supermarket

  19. Visualisering av farmakologiska substanser i levervävnad med hjälp av masspketrometrisk bildtagning

    OpenAIRE

    Mäkinen, Matias

    2017-01-01

    Målet för detta forskningsprojekt var att utveckla en metod med vars hjälp det är möjligt att detektera farmakologiska substanser i levervävnad med hjälp av masspektrometrisk bildtagning, så kallad MALDI-MSI. I litteraturen finns beskrivet tillämpningar av MALDI-MSI med vars hjälp det detekterats till exempel lipider i vävnader eller såsom i denna studie farmkologiska substanser. Arbetet bestod av två delmoment. I den första delen isolerades hepatocyter ur råttlever genom att perfusera k...

  20. Perfil lipídico, de aptidão cardiorrespiratória, e de composição corporal de uma amostra de dscolares de 8ª série de Canoas/RS Lipidic, cardiorespiratory fitness, and body composition profile of a sample of 8th graders from Canoas/RS

    Directory of Open Access Journals (Sweden)

    Mauren Lúcia de Araújo Bergmann

    2008-02-01

    meninas. Com relação às distribuições nas faixas recomendadas para cada variável, não houve associações com os sexos. Os resultados obtidos são preocupantes, e demonstram, mesmo em uma amostra pequena, a existência de todos os fatores de risco analisados para DC nos escolares.Nowadays cardiovascular diseases (CD are the main causes of death in the world and consist in a series of risk factors that seem to have their origin during childhood and adolescence. Thus, early diagnostic of CD risk factors should be frequently performed from young population. Therefore, the aim of this study was to describe the lipidic (LP, the cardiorespiratory fitness (CarFit, and the body composition (BC profile of 13/14 years old students, and compare these variables between sexes. The sample was composed of 41 students (21 boys and 20 girls 8th graders of a private school from Canoas city, RS, voluntarily selected in a non-probabilistic way. The LP (triglycerides - TRI; total cholesterol - TC; HDL and LDL of the students was measured through standard technique at a clinical analyses laboratory. The CarFit was measured through a 9- minute run/walk test (PROESP-BR, 2002. The BC was measured through the body mass index (BMI and by the sum of triceps and subscapular skinfolds (SSK. Descriptive analysis was used for data analysis and for the analysis per criteria the criteria suggested by AHA (NHI PARENT’S GUIDE, 1993 were used, for the CarFit and BD, the criteria suggested by PROESP-BR (2002 were used. The results pointed out that concerning central tendency analysis, the PL, the CarFit and the BC demonstrated normal results. However, when the analysis was made from the criteria, some cases out of the recommended band have been detected (TRI-4.9%, TC-12.2%, HDL-36.6% and LDL-4.9%, and a great proportion of students out of the recommended band for CarFit (61% and BC (BMI-17.1% and S SK 48.8%. Moreover, the results show that there are differences between sexes in the mean values of Car

  1. Insertion of apoLp-III into a lipid monolayer is more favorable for saturated, more ordered, acyl-chains

    Energy Technology Data Exchange (ETDEWEB)

    Rathnayake, Sewwandi S. [Kent State Univ., Kent, OH (United States); Mirheydari, Mona [Kent State Univ., Kent, OH (United States); Schulte, Adam [Kent State Univ., Kent, OH (United States); Gillahan, James E. [Kent State Univ., Kent, OH (United States); Gentit, Taylor [Kent State Univ., Kent, OH (United States); Phillips, Ashley N. [Kent State Univ., Kent, OH (United States); Okonkwo, Rose K. [Kent State Univ., Kent, OH (United States); Burger, Koert N.J. [Utrecht Univ. (Netherlands); Mann, Elizabeth K. [Kent State Univ., Kent, OH (United States); Vaknin, David [Ames Lab., Ames, IA (United States); Bu, Wei [Ames Lab., Ames, IA (United States); Agra-Kooijman, Dena Mae [Kent State Univ., Kent, OH (United States); Kooijman, Edgar E. [Kent State Univ., Kent, OH (United States)

    2013-10-04

    Neutral lipid transport in mammals is complicated involving many types of apolipoprotein. The exchangeable apolipoproteins mediate the transfer of hydrophobic lipids between tissues and particles, and bind to cell surface receptors. Amphipathic a-helices form a common structural motif that facilitates their lipid binding and exchangeability. ApoLp-III, the only exchangeable apolipoprotein found in insects, is a model amphipathic a:helix bundle protein and its three dimensional structure and function mimics that of the mammalian proteins apoE and apoAI. Even the intracellular exchangeable lipid droplet protein TIP47/perilipin 3 contains an a-helix bundle domain with high structural similarity to that of apoE and apoLp-III. Here, we investigated the interaction of apoLp-III from Locusta migratoria with lipid monolayers. Consistent with earlier work we find that insertion of apoLp-III into fluid lipid monolayers is highest for diacylglycerol. We observe a preference for saturated and more highly ordered lipids, suggesting a new mode of interaction for amphipathic a-helix bundles. X-ray reflectivity shows that apoLp-III unfolds at a hydrophobic interface and flexible loops connecting the amphipathic cc-helices stay in solution. X-ray diffraction indicates that apoLp-III insertion into diacylglycerol monolayers induces additional ordering of saturated acyl-chains. These results thus shed important new insight into the protein-lipid interactions of a model exchangeable apolipoprotein with significant implications for its mammalian counterparts. (C) 2013 Elsevier B.V. All rights reserved.

  2. Genome-wide association study of Lp-PLA(2 activity and mass in the Framingham Heart Study.

    Directory of Open Access Journals (Sweden)

    Sunil Suchindran

    2010-04-01

    Full Text Available Lipoprotein-associated phospholipase A(2 (Lp-PLA(2 is an emerging risk factor and therapeutic target for cardiovascular disease. The activity and mass of this enzyme are heritable traits, but major genetic determinants have not been explored in a systematic, genome-wide fashion. We carried out a genome-wide association study of Lp-PLA(2 activity and mass in 6,668 Caucasian subjects from the population-based Framingham Heart Study. Clinical data and genotypes from the Affymetrix 550K SNP array were obtained from the open-access Framingham SHARe project. Each polymorphism that passed quality control was tested for associations with Lp-PLA(2 activity and mass using linear mixed models implemented in the R statistical package, accounting for familial correlations, and controlling for age, sex, smoking, lipid-lowering-medication use, and cohort. For Lp-PLA(2 activity, polymorphisms at four independent loci reached genome-wide significance, including the APOE/APOC1 region on chromosome 19 (p = 6 x 10(-24; CELSR2/PSRC1 on chromosome 1 (p = 3 x 10(-15; SCARB1 on chromosome 12 (p = 1x10(-8 and ZNF259/BUD13 in the APOA5/APOA1 gene region on chromosome 11 (p = 4 x 10(-8. All of these remained significant after accounting for associations with LDL cholesterol, HDL cholesterol, or triglycerides. For Lp-PLA(2 mass, 12 SNPs achieved genome-wide significance, all clustering in a region on chromosome 6p12.3 near the PLA2G7 gene. Our analyses demonstrate that genetic polymorphisms may contribute to inter-individual variation in Lp-PLA(2 activity and mass.

  3. Influence of fruit drinks with or without lactobacillus Lp299v on the gastrointestinal uptake of paracetamol in man

    DEFF Research Database (Denmark)

    Akerman, Ulrika; Edvinsson, Lars

    2009-01-01

    (a 95% confidence interval of 57,161). The corresponding 30 minutes values of paracetamol levels in the presence of rosehip with or without Lp299v were 0 mumol/l (95% confidence intervals contain only zero for both rosehip treatments). There were significant differences in AUC, maximal paracetamol...... = 0.003); there were no significant difference between rosehip drink with or without Lp299v (p > 0.3). CONCLUSION: We have demonstrated an interaction between the uptake of paracetamol and the solvent in rosehip drink/Provia which mainly consists of long chain carbohydrates. This may in the clinic...

  4. Single-nucleotide polymorphisms in LPA explain most of the ancestry-specific variation in Lp(a levels in African Americans.

    Directory of Open Access Journals (Sweden)

    Rahul C Deo

    2011-01-01

    Full Text Available Lipoprotein(a (Lp(a is an important causal cardiovascular risk factor, with serum Lp(a levels predicting atherosclerotic heart disease and genetic determinants of Lp(a levels showing association with myocardial infarction. Lp(a levels vary widely between populations, with African-derived populations having nearly 2-fold higher Lp(a levels than European Americans. We investigated the genetic basis of this difference in 4464 African Americans from the Jackson Heart Study (JHS using a panel of up to 1447 ancestry informative markers, allowing us to accurately estimate the African ancestry proportion of each individual at each position in the genome. In an unbiased genome-wide admixture scan for frequency-differentiated genetic determinants of Lp(a level, we found a convincing peak (LOD = 13.6 at 6q25.3, which spans the LPA locus. Dense fine-mapping of the LPA locus identified a number of strongly associated, common biallelic SNPs, a subset of which can account for up to 7% of the variation in Lp(a level, as well as >70% of the African-European population differences in Lp(a level. We replicated the association of the most strongly associated SNP, rs9457951 (p = 6 × 10(-22, 27% change in Lp(a per allele, ∼5% of Lp(a variance explained in JHS, in 1,726 African Americans from the Dallas Heart Study and found an even stronger association after adjustment for the kringle(IV repeat copy number. Despite the strong association with Lp(a levels, we find no association of any LPA SNP with incident coronary heart disease in 3,225 African Americans from the Atherosclerosis Risk in Communities Study.

  5. Perturbations in the Lipid Profile of Individuals with Newly Diagnosed Type 1 Diabetes Mellitus: Lipidomics Analysis of a Diabetes Antibody Standardization Program Sample Subset

    Energy Technology Data Exchange (ETDEWEB)

    Sorensen, Christina M.; Ding, Jie; Zhang, Qibin; Alquier, Thierry; Zhao, Rui; Mueller, Patricia W.; Smith, Richard D.; Metz, Thomas O.

    2010-08-01

    Objectives: To characterize the lipid profile of individuals with newly diagnosed type 1 diabetes mellitus using LC-MS-based lipidomics and the accurate mass and time (AMT) tag approach. Design and methods: Lipids were extracted from plasma and sera of 10 subjects from the Diabetes Antibody Standardization Program (years 2000-2005) and 10 non-diabetic subjects and analyzed by capillary liquid chromatography coupled with a hybrid ion-trap-Fourier transform ion cyclotron resonance mass spectrometer. Lipids were identified and quantified using the AMT tag approach. Results: Five hundred sixty lipid features differentiated (q < 0.05) diabetic from healthy individuals in a partial least-squares analysis, characterizing of individuals with recently diagnosed type 1 diabetes mellitus. Conclusions: A lipid profile associated with newly diagnosed type 1 diabetes may aid in further characterization of biochemical pathways involved in lipid regulation or mobilization and lipotoxicity of pancreatic beta-cells.

  6. Ultra-processed foods have the worst nutrient profile, yet they are the most available packaged products in a sample of New Zealand supermarkets.

    Science.gov (United States)

    Luiten, Claire M; Steenhuis, Ingrid Hm; Eyles, Helen; Ni Mhurchu, Cliona; Waterlander, Wilma E

    2016-02-01

    To examine the availability of packaged food products in New Zealand supermarkets by level of industrial processing, nutrient profiling score (NPSC), price (energy, unit and serving costs) and brand variety. Secondary analysis of cross-sectional survey data on packaged supermarket food and non-alcoholic beverages. Products were classified according to level of industrial processing (minimally, culinary and ultra-processed) and their NPSC. Packaged foods available in four major supermarkets in Auckland, New Zealand. Packaged supermarket food products for the years 2011 and 2013. The majority (84% in 2011 and 83% in 2013) of packaged foods were classified as ultra-processed. A significant positive association was found between the level of industrial processing and NPSC, i.e., ultra-processed foods had a worse nutrient profile (NPSC=11.63) than culinary processed foods (NPSC=7.95), which in turn had a worse nutrient profile than minimally processed foods (NPSC=3.27), Pprocessing. The study observed many variations of virtually the same product. The ten largest food manufacturers produced 35% of all packaged foods available. In New Zealand supermarkets, ultra-processed foods comprise the largest proportion of packaged foods and are less healthy than less processed foods. The lack of significant price difference between ultra- and less processed foods suggests ultra-processed foods might provide time-poor consumers with more value for money. These findings highlight the need to improve the supermarket food supply by reducing numbers of ultra-processed foods and by reformulating products to improve their nutritional profile.

  7. Coupled-Flow Simulation of HP-LP Turbines Has Resulted in Significant Fuel Savings

    Science.gov (United States)

    Veres, Joseph P.

    2001-01-01

    Our objective was to create a high-fidelity Navier-Stokes computer simulation of the flow through the turbines of a modern high-bypass-ratio turbofan engine. The simulation would have to capture the aerodynamic interactions between closely coupled high- and low-pressure turbines. A computer simulation of the flow in the GE90 turbofan engine's high-pressure (HP) and low-pressure (LP) turbines was created at GE Aircraft Engines under contract with the NASA Glenn Research Center. The three-dimensional steady-state computer simulation was performed using Glenn's average-passage approach named APNASA. The areas upstream and downstream of each blade row mutually interact with each other during engine operation. The embedded blade row operating conditions are modeled since the average passage equations in APNASA actively include the effects of the adjacent blade rows. The turbine airfoils, platforms, and casing are actively cooled by compressor bleed air. Hot gas leaks around the tips of rotors through labyrinth seals. The flow exiting the high work HP turbines is partially transonic and, therefore, has a strong shock system in the transition region. The simulation was done using 121 processors of a Silicon Graphics Origin 2000 (NAS 02K) cluster at the NASA Ames Research Center, with a parallel efficiency of 87 percent in 15 hr. The typical average-passage analysis mesh size per blade row was 280 by 45 by 55, or approx.700,000 grid points. The total number of blade rows was 18 for a combined HP and LP turbine system including the struts in the transition duct and exit guide vane, which contain 12.6 million grid points. Design cycle turnaround time requirements ran typically from 24 to 48 hr of wall clock time. The number of iterations for convergence was 10,000 at 8.03x10(exp -5) sec/iteration/grid point (NAS O2K). Parallel processing by up to 40 processors is required to meet the design cycle time constraints. This is the first-ever flow simulation of an HP and LP

  8. Analisis Kata Tabu dan Klasifikasinya di Lirik Lagu Eminem pada Album `The Marshal Mathers LP`

    Directory of Open Access Journals (Sweden)

    Laily Nur Affini

    2017-04-01

    Full Text Available This research is dedicated for readers in the field of linguistics and has a purpose to reveal taboo words based on a certain theory and the classifications. The analysed taboo words exist in Emeninems album,The Marshall Mathers LP. A theory employed in the analysis is using Timothy Jay theory where taboo words are differentiated in seven classifications; cursing, profanity, blasphemy, obscenity, sexual harassment, vulgar language, name-calling and insult.The data was taken into two parts, primary and secondary. The primary data is the lyric itself and the secondary data is taken from books, articles and dictionary. The result of the analysis shows a revelation of the taboo words classifications, shown up in a table.

  9. 78 FR 13742 - BB&T Capital Partners Mezzanine Fund II, L.P.; Notice Seeking Exemption Under Section 312 of the...

    Science.gov (United States)

    2013-02-28

    ... ADMINISTRATION BB&T Capital Partners Mezzanine Fund II, L.P.; Notice Seeking Exemption Under Section 312 of the Small Business Investment Act, Conflicts of Interest Notice is hereby given that BB&T Capital Partners... Regulations (13 CFR 107.730). BB&T Capital Partners Mezzanine Fund II, L.P. proposes to provide debt financing...

  10. 77 FR 76586 - GC SBIC IV, L.P.; Notice Seeking Exemption Under Section 312 of the Small Business Investment Act...

    Science.gov (United States)

    2012-12-28

    ... Regulations (13 CFR 107.730). GC SBIC IV, L.P. provided loan financing to Massage Envy LLC, 14350 N. 87th... the Regulations because Massage Envy LLC used financing proceeds from GC SBIC IV, L.P. in part to...

  11. 77 FR 74545 - Eagle Fund III-A, L.P.; License No. 07/07-0117: Notice Seeking Exemption Under Section 312 of the...

    Science.gov (United States)

    2012-12-14

    ... ADMINISTRATION Eagle Fund III-A, L.P.; License No. 07/07-0117: Notice Seeking Exemption Under Section 312 of the Small Business Investment Act, Conflicts of Interest Notice is hereby given that Eagle Fund III-A, L.P... interest, of the Small Business Administration Rules and Regulations (13 CFR part 107). Eagle Fund III- A...

  12. 77 FR 70812 - United States v. Star Atlantic Waste Holdings, L.P., Veolia Environnement S.A. and Veolia ES...

    Science.gov (United States)

    2012-11-27

    ... Antitrust Division United States v. Star Atlantic Waste Holdings, L.P., Veolia Environnement S.A. and Veolia... Environnement S.A. and Veolia ES Solid Waste, Inc., Civil Action No. 1:12-cv-01847-RWR. On November 15, 2012... Holdings, L.P. of Veolia Environnement S.A.'s U.S. subsidiary, Veolia ES Solid Waste, Inc., would violate...

  13. A Novel Low-Temperature Alkaline Lipase from Acinetobacter johnsonii LP28 Suitable for Detergent Formulation

    Directory of Open Access Journals (Sweden)

    Hai Kuan Wang

    2011-01-01

    Full Text Available A strain LP28 that produces alkaline and low-temperature lipase was isolated from the soil collected from the Bay of Bohai, PR China and identified as Acinetobacter johnsonii using 16S rDNA sequencing. The lipase was purified to homogeneity by centrifugation, followed by ammonium sulphate precipitation, dialysis, ion exchange chromatography on cellulose DE-52 and gel filtration chromatography on Sephadex G-75. The enzyme was purified about 34-fold with a final yield of 13 % and the relative molecular mass of the enzyme was determined to be 53 kDa by SDS-PAGE. The purified enzyme exhibited maximum activity at 30 °C and pH=9.0, and retained 94.53 % of its maximum activity at 20 °C. The enzyme was stable at 50 °C and retained 80.9 % of its original activity for 30 min. It was also highly stable in a pH range of 8.0–11.0. The enzyme hydrolyzed a wide range of oils and showed a high level of lipase activity in hydrolyzing tributyrin. The enzyme activity was promoted in the presence of Na+, Ca2+, K+, Mg2+ and sodium citrate. Ba2+, Mn2+, Cr3+ and Co2+ did not affect the enzyme activity, whereas the presence of Al3+, Cu2+, Fe2+, Fe3+, Zn2+ and EDTA reduced the enzyme activity. Regarding the stability of detergent process, the enzyme was highly stable in the presence of various oxidizing agents, some commercial detergents and alkaline protease, and its activity was also promoted by most of the surfactants, viz. Tween 20, Tween 80, sodium cholate, sodium taurocholate and saponin. For these characteristics, the lipase from Acinetobacter johnsonii LP28 showed good potential as an additive in laundry detergent formulation.

  14. Effects of Supportive Educational Program on Anxiety of Mothers of Children Undergoing the Lumbar Puncture (LP

    Directory of Open Access Journals (Sweden)

    Mousa Mahdizadeh

    2016-07-01

    Full Text Available Background: Lumbar Puncture test is one of the most stressful diagnostic procedures in pediatric ward that its prescription put mothers under extreme pressure. There are limited interventions in this regard. Aim: determining the impact of supportive educational program on the anxiety of mothers whose children are undergoing lumbar puncture (LP. Method: the present study is a randomized controlled clinical trial carried out in 2015. Sixty mothers of children undergoing lumbar puncture and hospitalized 3educational hospitals of Mashhad, Iran, were randomly divided to control group (n=30 and intervention group (n=30. The intervention group received supportive program during a session of 60 min. Spielberger State-Trait Anxiety Inventory (STAI was selected as the research tool. Finally, data were analyzed by Chi-square test, independent t-test using SPSS v.16 software. Results: The mean age of the mothers was 27.3±5.3 and 26.0±3.1, respectively. The mean pre-interventions maternal manifest anxiety was 51.9±6.2 and 53.4±4.3 out of 80, respectively, in the intervention and control groups. The mean post-interventions maternal manifest anxiety was 44.9±5.1 and 52.7±4.2, respectively, in the intervention and control groups. According to the results of independent t-test, significant difference was observed statistically for the mean maternal manifest anxiety between the two groups (p> 0.001. Implications for Practice: Regarding the reduction of maternal anxiety in the intervention group, which is possible because of the attention to the emotional needs of mothers, in addition to proper training; this program can be considered as an effective method to reduce maternal anxiety before the LP test.

  15. Characterization of learning and memory deficits in C57BL/6 mice infected with LP-BM5, a murine model of AIDS.

    Science.gov (United States)

    Iida, R; Yamada, K; Mamiya, T; Saito, K; Seishima, M; Nabeshima, T

    1999-03-01

    Mice infected with an immunosuppressive murine leukemia virus mixture, LP-BM5 show a profound immunosuppression described as murine acquired immune deficiency syndrome (AIDS). In the present study, we characterized learning and memory deficits in C57BL/6 mice infected with LP-BM5. Spontaneous alternation behavior in a Y-maze and latent learning (spatial attention) in a water-finding test, as well as spatial reference and reversal learning in a water maze test, were significantly impaired in the mice infected with LP-BM5. These deficits appeared in the absence of any motoric and visual impairment as assessed by open-field, rotarod and visual water maze tests. These results suggest that cognitive functions are impaired in the mice infected with LP-BM5. Furthermore, LP-BM5-infected mice may be useful as a model for the AIDS dementia complex.

  16. 75 FR 76037 - HAVI Logistics, North America a Subsidiary of HAVI Group, LP Including On-Site Leased Workers of...

    Science.gov (United States)

    2010-12-07

    ... Employment and Training Administration HAVI Logistics, North America a Subsidiary of HAVI Group, LP Including... Logistics, North America, Lisle, IL; Amended Certification Regarding Eligibility To Apply for Worker... Assistance on September 3, 2010, applicable to workers of HAVI Logistics, North America, a subsidiary of HAVI...

  17. 78 FR 28273 - Alpine Investors IV SBIC, LP; Notice Seeking Exemption Under Section 312 of the Small Business...

    Science.gov (United States)

    2013-05-14

    ... Investment Act, Conflicts of Interest Notice is hereby given that Alpine Investors IV SBIC, LP, 3 Embarcadero Center, Suite 2330, San Francisco, CA, a Federal Licensee under the Small Business Investment Act of 1958... percent of the stock of Great Bay Software, Inc., to fund transaction fees, and for working capital...

  18. 77 FR 24723 - AstraZeneca Pharmaceuticals LP; Withdrawal of Approval of a New Drug Application for IRESSA

    Science.gov (United States)

    2012-04-25

    ... HUMAN SERVICES Food and Drug Administration AstraZeneca Pharmaceuticals LP; Withdrawal of Approval of a New Drug Application for IRESSA AGENCY: Food and Drug Administration, HHS. ACTION: Notice. SUMMARY: The Food and Drug Administration (FDA) is withdrawing approval of a new drug application (NDA) for...

  19. Optimal design for crosstalk analysis in 12-core 5-LP mode homogeneous multicore fiber for different lattice structure

    Science.gov (United States)

    Kumar, Dablu; Ranjan, Rakesh

    2018-03-01

    12-Core 5-LP mode homogeneous multicore fibers have been proposed for analysis of inter-core crosstalk and dispersion, with four different lattice structures (circular, 2-ring, square lattice, and triangular lattice) having cladding diameter of 200 μm and a fixed cladding thickness of 35 μm. The core-to-core crosstalk impact has been studied numerically with respect to bending radius, core pitch, transmission distance, wavelength, and core diameter for all 5-LP modes. In anticipation of further reduction in crosstalk levels, the trench-assisted cores have been incorporated for all respective designs. Ultra-low crosstalk (-138 dB/100 km) has been achieved through the triangular lattice arrangement, with trench depth Δ2 = -1.40% for fundamental (LP01) mode. It has been noted that the impact of mode polarization on crosstalk behavior is minor, with difference in crosstalk levels between two polarized spatial modes as ≤0.2 dB. Moreover, the optimized cladding diameter has been obtained for all 5-LP modes for a target value of crosstalk of -50 dB/100 km, with all the core arrangements. The dispersion characteristic has also been analyzed with respect to wavelength, which is nearly 2.5 ps/nm km at operating wavelength 1550 nm. The relative core multiplicity factor (RCMF) for the proposed design is obtained as 64.

  20. 75 FR 76040 - Dell Products LP, Winston-Salem (WS-1) Division, Including On-Site Leased Workers From Adecco...

    Science.gov (United States)

    2010-12-07

    ... Resources and Recovery, SecurAmerica, LLC, Industrial Distribution Group (IDG), LLC, ARM Automation, Inc... Employment and Training Administration Dell Products LP, Winston-Salem (WS-1) Division, Including On- Site... Solutions, South East, Omni Resources and Recovery, SecurAmerica, LLC, Industrial Distribution Group (IDG...

  1. 76 FR 21034 - Dell Products LP, Winston-Salem (WS-1) Division, Including On-Site Leased Workers From Adecco...

    Science.gov (United States)

    2011-04-14

    ... Employment and Training Administration Dell Products LP, Winston-Salem (WS-1) Division, Including On- Site... Solutions, South East, Omni Resources And Recovery, Securamerica, LLC, Industrial Distribution Group (IDG), LLC, Arm Automation, Inc., Seaton Corporation, Foxconn/PCE Technology, Inc. and Select Staffing, Also...

  2. 76 FR 76475 - Tregaron Opportunity Fund I, L.P.; Notice Seeking Exemption Under Section 312 of the Small...

    Science.gov (United States)

    2011-12-07

    ... Business Investment Act, Conflicts of Interest Notice is hereby given that Tregaron Opportunity Fund I, L.P., 540 University Avenue, Suite 250, Palo Alto, CA 94301, a Federal Licensee under the Small Business... From the Federal Register Online via the Government Publishing Office SMALL BUSINESS...

  3. 78 FR 48177 - Purdue Pharma L.P.; Withdrawal of Approval of a New Drug Application for Oxycontin

    Science.gov (United States)

    2013-08-07

    .... FDA-2013-N-0883] Purdue Pharma L.P.; Withdrawal of Approval of a New Drug Application for Oxycontin...) is withdrawing approval of a new drug application (NDA) for OXYCONTIN (oxycodone hydrochloride...: FDA approved NDA 20-553 for OXYCONTIN (oxycodone hydrochloride) Extended-Release Tablets, 10...

  4. 76 FR 54758 - Alliance Pipeline L.P.; Notice of Intent To Prepare an Environmental Assessment for the Planned...

    Science.gov (United States)

    2011-09-02

    ... the Commission's pre-filing process. A flow chart illustrating the pre-filing process is included with... Project involving construction and operation of facilities by Alliance Pipeline L.P. (Alliance) in... Commission in its decision-making process to determine whether the project is in the public convenience and...

  5. 76 FR 51966 - Texas Eastern Transmission, LP; Notice of Intent To Prepare an Environmental Assessment for the...

    Science.gov (United States)

    2011-08-19

    ... not your only public input opportunity; please refer to the Environmental Review Process flow chart in... Expansion Project involving construction and operation of facilities by Texas Eastern Transmission, LP...- making process to determine whether the project is in the public convenience and necessity. This notice...

  6. 76 FR 63278 - Bayer CropScience LP; Determination of Nonregulated Status for Cotton Genetically Engineered for...

    Science.gov (United States)

    2011-10-12

    ... Animal and Plant Health Inspection Service [Docket No. APHIS-2010-0102] Bayer CropScience LP... Tolerance AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION: Notice. SUMMARY: We are advising... Through Genetic Engineering Which Are Plant Pests or Which There Is Reason to Believe Are Plant Pests...

  7. 77 FR 36525 - Cheniere Creole Trail Pipeline Company, L.P.; Notice of Intent To Prepare an Environmental...

    Science.gov (United States)

    2012-06-19

    ... Energy Regulatory Commission Cheniere Creole Trail Pipeline Company, L.P.; Notice of Intent To Prepare an Environmental Assessment for the Proposed Creole Trail Expansion Project and Request for Comments on... environmental assessment (EA) that will discuss the environmental impacts of the Creole Trail Expansion Project...

  8. 78 FR 66911 - Texas Eastern Transmission, LP; Notice of Intent To Prepare an Environmental Assessment for the...

    Science.gov (United States)

    2013-11-07

    ... discuss the environmental impacts of the Emerald Longwall Mine Panel D1 Project in Docket No. CP14-4-000... Energy Regulatory Commission Texas Eastern Transmission, LP; Notice of Intent To Prepare an Environmental Assessment for the Proposed Emerald Longwall Mine Panel D1 and Consol Baily East Mine Panel 1l Projects and...

  9. 78 FR 77770 - Ironwood Equity Fund, LP; Notice Seeking Exemption Under Section 312 of the Small Business...

    Science.gov (United States)

    2013-12-24

    ... Investment Act, Conflicts of Interest Notice is hereby given that Ironwood Equity Fund, LP, 45 Nod Road, Avon... Act and Section 107.730, Financings which Constitute Conflicts of Interest of the Small Business... convertible debt investment in Action Environmental Group, Inc., a portfolio company of its Associate Ironwood...

  10. 75 FR 6414 - Cardinal Growth, L.P.; Notice Seeking Exemption Under Section 312 of the Small Business...

    Science.gov (United States)

    2010-02-09

    ... Act, Conflicts of Interest Notice is hereby given that Cardinal Growth, L.P., 311 South Wacker Driver... Section 312 of the Act and Section 107.730, Financings which Constitute Conflicts of Interest of the Small... financing to GreenRock Environmental Management, Inc., 1400 West Carroll Avenue, Chicago, IL 60607. The...

  11. 76 FR 77298 - Emergence Capital Partners SBIC, L.P.; Notice Seeking Exemption Under Section 312 of the Small...

    Science.gov (United States)

    2011-12-12

    ... Business Investment Act, Conflicts of Interest Notice is hereby given that Ironwood Mezzanine Fund II, L.P... Section 312 of the Act and Section 107.730, Financings which Constitute Conflicts of Interest of the Small.... proposes to provide debt financing to Action Environmental Group, Inc., 451 Frelinghuysen Avenue, Newark...

  12. 75 FR 5634 - Ironwood Mezzanine Fund II, L.P.; Notice Seeking Exemption Under Section 312 of the Small...

    Science.gov (United States)

    2010-02-03

    ... Investment Act, Conflicts of Interest Notice is hereby given that Ironwood Mezzanine Fund II, L.P. 200 Fisher... Section 312 of the Act and Section 107.730, Financings which Constitute Conflicts of Interest of the Small.... proposes to provide debt/equity security financing to Action Carting Environmental Services, Inc., 451...

  13. 76 FR 36914 - Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas...

    Science.gov (United States)

    2011-06-23

    ... Energy Regulatory Commission Astoria Generating Company, L.P., NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas Turbine Power LLC, Dunkirk Power LLC, Huntley Power LLC, Oswego Harbor Power LLC, TC..., 2011, Astoria Generating Company, NRG Power Marketing LLC, Arthur Kill Power LLC, Astoria Gas Turbine...

  14. Enterotoxigenic profiling of emetic toxin- and enterotoxin-producing Bacillus cereus, Isolated from food, environmental, and clinical samples by multiplex PCR.

    Science.gov (United States)

    Forghani, Fereidoun; Kim, Jung-Beom; Oh, Deog-Hwan

    2014-11-01

    Bacillus cereus comprises the largest group of endospore-forming bacteria and can cause emetic and diarrheal food poisoning. A total of 496 B. cereus strains isolated from various sources (food, environmental, clinical) were assessed by a multiplex PCR for the presence of enterotoxin genes. The detection rate of nheA, entFM, hblC, and cytK enterotoxin genes among all B. cereus strains was 92.33%, 77.21%, 59.47%, and 47.58%, respectively. Enterotoxigenic profiles were determined in emetic toxin- (8 patterns) and enterotoxin-producing strains (12 patterns). The results provide important information on toxin prevalence and toxigenic profiles of B. cereus from various sources. Our findings revealed that B. cereus must be considered a serious health hazard and Bacillus thuringiensis should be considered of a greater potential concern to food safety among all B. cereus group members. Also, there is need for intensive and continuous monitoring of products embracing both emetic toxin and enterotoxin genes. © 2014 Institute of Food Technologists®

  15. Relative effectiveness of kinetic analysis vs single point readings for classifying environmental samples based on community-level physiological profiles (CLPP)

    Science.gov (United States)

    Garland, J. L.; Mills, A. L.; Young, J. S.

    2001-01-01

    The relative effectiveness of average-well-color-development-normalized single-point absorbance readings (AWCD) vs the kinetic parameters mu(m), lambda, A, and integral (AREA) of the modified Gompertz equation fit to the color development curve resulting from reduction of a redox sensitive dye from microbial respiration of 95 separate sole carbon sources in microplate wells was compared for a dilution series of rhizosphere samples from hydroponically grown wheat and potato ranging in inoculum densities of 1 x 10(4)-4 x 10(6) cells ml-1. Patterns generated with each parameter were analyzed using principal component analysis (PCA) and discriminant function analysis (DFA) to test relative resolving power. Samples of equivalent cell density (undiluted samples) were correctly classified by rhizosphere type for all parameters based on DFA analysis of the first five PC scores. Analysis of undiluted and 1:4 diluted samples resulted in misclassification of at least two of the wheat samples for all parameters except the AWCD normalized (0.50 abs. units) data, and analysis of undiluted, 1:4, and 1:16 diluted samples resulted in misclassification for all parameter types. Ordination of samples along the first principal component (PC) was correlated to inoculum density in analyses performed on all of the kinetic parameters, but no such influence was seen for AWCD-derived results. The carbon sources responsible for classification differed among the variable types with the exception of AREA and A, which were strongly correlated. These results indicate that the use of kinetic parameters for pattern analysis in CLPP may provide some additional information, but only if the influence of inoculum density is carefully considered. c2001 Elsevier Science Ltd. All rights reserved.

  16. Triacylglycerol Analysis in Human Milk and Other Mammalian Species: Small-Scale Sample Preparation, Characterization, and Statistical Classification Using HPLC-ELSD Profiles.

    Science.gov (United States)

    Ten-Doménech, Isabel; Beltrán-Iturat, Eduardo; Herrero-Martínez, José Manuel; Sancho-Llopis, Juan Vicente; Simó-Alfonso, Ernesto Francisco

    2015-06-24

    In this work, a method for the separation of triacylglycerols (TAGs) present in human milk and from other mammalian species by reversed-phase high-performance liquid chromatography using a core-shell particle packed column with UV and evaporative light-scattering detectors is described. Under optimal conditions, a mobile phase containing acetonitrile/n-pentanol at 10 °C gave an excellent resolution among more than 50 TAG peaks. A small-scale method for fat extraction in these milks (particularly of interest for human milk samples) using minimal amounts of sample and reagents was also developed. The proposed extraction protocol and the traditional method were compared, giving similar results, with respect to the total fat and relative TAG contents. Finally, a statistical study based on linear discriminant analysis on the TAG composition of different types of milks (human, cow, sheep, and goat) was carried out to differentiate the samples according to their mammalian origin.

  17. Metabolite profiling of small cerebrospinal fluid sample volumes with gas chromatography-mass spectrometry: application to a rat model of Multiple Sclerosis

    NARCIS (Netherlands)

    Coulier, L.; Muilwijk, B.; Bijlsma, S.; Noga, M.; Tienstra, M.; Attali, A.; Aken, H. van; Suidgeest, E.; Tuinstra, T.; Hankemeier, T.; Bobeldijk, I.

    2012-01-01

    Analysis of metabolites in biofluids by gas chromatography–mass spectrometry (GC–MS) after oximation and silylation is a key method in metabolomics. The GC–MS method was modified by a modified vial design and sample work-up procedure in order to make the method applicable to small volumes of

  18. Metabolite profiling of small cerebrospinal fluid sample volumes with gas chromatography-mass spectrometry: Application to a rat model of multiple sclerosis

    NARCIS (Netherlands)

    Coulier, L.; Muilwijk, B.; Bijlsma, S.; Noga, M.; Tienstra, M.; Attali, A.; Aken, H. van; Suidgeest, E.; Tuinstra, T.; Luider, T.M.; Hankemeier, T.; Bobeldijk, I.

    2013-01-01

    Analysis of metabolites in biofluids by gas chromatography-mass spectrometry (GC-MS) after oximation and silylation is a key method in metabolomics. The GC-MS method was modified by a modified vial design and sample work-up procedure in order to make the method applicable to small volumes of

  19. Effects of DNA Extraction Procedures on Bacteroides Profiles in Fecal Samples From Various Animals Determined by Terminal Restriction Fragment Length Polymorphism Analysis

    Science.gov (United States)

    A major assumption in microbial source tracking is that some fecal bacteria are specific to a host animal, and thus provide unique microbial fingerprints that can be used to differentiate hosts. However, the DNA information obtained from a particular sample may be biased dependi...

  20. A Novel Method for Profiling and Quantifying Short- and Medium-Chain Chlorinated Paraffins in Environmental Samples Using Comprehensive Two-Dimensional Gas Chromatography-Electron Capture Negative Ionization High-Resolution Time-of-Flight Mass Spectrometry.

    Science.gov (United States)

    Xia, Dan; Gao, Lirong; Zheng, Minghui; Tian, Qichang; Huang, Huiting; Qiao, Lin

    2016-07-19

    Chlorinated paraffins (CPs) are complex technical mixtures containing thousands of isomers. Analyzing CPs in environmental matrices is extremely challenging. CPs have broad, unresolved profiles when analyzed by one-dimensional gas chromatography (GC). Comprehensive two-dimensional GC (GC×GC) can separate CPs with a high degree of orthogonality. A novel method for simultaneously profiling and quantifying short- and medium-chain CPs, using GC×GC coupled with electron capture negative ionization high-resolution time-of-flight mass spectrometry, was developed. The method allowed 48 CP formula congener groups to be analyzed highly selectively in one injection through accurate mass measurements of the [M - Cl](-) ions in full scan mode. The correlation coefficients (R(2)) for the linear calibration curves for different chlorine contents were 0.982 for short-chain CPs and 0.945 for medium-chain CPs. The method was successfully used to determine CPs in sediment and fish samples. By using this method, with enhanced chromatographic separation and high mass resolution, interferences between CP congeners and other organohalogen compounds, such as toxaphene, are minimized. New compounds, with the formulas C9H14Cl6 and C9H13Cl7, were found in sediment and biological samples for the first time. The method was shown to be a powerful tool for the analysis of CPs in environmental samples.

  1. Culture conditions and sample preparation methods affect spectrum quality and reproducibility during profiling of Staphylococcus aureus with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

    Science.gov (United States)

    Goldstein, J E; Zhang, L; Borror, C M; Rago, J V; Sandrin, T R

    2013-08-01

    Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a promising tool to rapidly characterize Staphylococcus aureus. Different protocols have been employed, but effects of experimental factors, such as culture condition and sample preparation, on spectrum quality and reproducibility have not been rigorously examined. We applied MALDI-TOF MS to characterize a model system consisting of five methicillin-sensitive (MSSA) and five methicillin-resistant S. aureus isolates (MRSA) under two culture conditions (agar and broth) and using two sample preparation methods [intact cell method and protein extraction method (PEM)]. The effects of these treatments on spectrum quality and reproducibility were quantified. PEM facilitated increases in the number of peaks and mass range width. Broth cultures further improved spectrum quality in terms of increasing the number of peaks. In addition, PEM increased reproducibility in samples prepared using identical culture conditions. MALDI imaging data suggested that the improvement in reproducibility may result from a more homogeneous distribution of sample associated with the broth/PEM treatment. Broth/PEM treatment also yielded the highest rate (96%) of correct classification for MRSA. Taken together, these results suggest that broth/PEM maximizes the performance of MALDI-TOF MS to characterize S. aureus. Two culture conditions (agar or broth) and two sample preparation methods (intact cell or protein extraction) were evaluated for their effects on profiling of Staphylococcus aureus using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Results indicated that MALDI-enabled profiling of S. aureus is most effective when cultures are grown in broth and processed using a protein extraction-based approach. These findings should enhance future efforts to maximize the performance of this approach to characterize strains of S. aureus. © 2013

  2. Profiling of adrenocorticotropic hormone and arginine vasopressin in human pituitary gland and tumor thin tissue sections using droplet-based liquid-microjunction surface-sampling-HPLC-ESI-MS-MS.

    Science.gov (United States)

    Kertesz, Vilmos; Calligaris, David; Feldman, Daniel R; Changelian, Armen; Laws, Edward R; Santagata, Sandro; Agar, Nathalie Y R; Van Berkel, Gary J

    2015-08-01

    Described here are the results from the profiling of the proteins arginine vasopressin (AVP) and adrenocorticotropic hormone (ACTH) from normal human pituitary gland and pituitary adenoma tissue sections, using a fully automated droplet-based liquid-microjunction surface-sampling-HPLC-ESI-MS-MS system for spatially resolved sampling, HPLC separation, and mass spectrometric detection. Excellent correlation was found between the protein distribution data obtained with this method and data obtained with matrix-assisted laser desorption/ionization (MALDI) chemical imaging analyses of serial sections of the same tissue. The protein distributions correlated with the visible anatomic pattern of the pituitary gland. AVP was most abundant in the posterior pituitary gland region (neurohypophysis), and ATCH was dominant in the anterior pituitary gland region (adenohypophysis). The relative amounts of AVP and ACTH sampled from a series of ACTH-secreting and non-secreting pituitary adenomas correlated with histopathological evaluation. ACTH was readily detected at significantly higher levels in regions of ACTH-secreting adenomas and in normal anterior adenohypophysis compared with non-secreting adenoma and neurohypophysis. AVP was mostly detected in normal neurohypophysis, as expected. This work reveals that a fully automated droplet-based liquid-microjunction surface-sampling system coupled to HPLC-ESI-MS-MS can be readily used for spatially resolved sampling, separation, detection, and semi-quantitation of physiologically-relevant peptide and protein hormones, including AVP and ACTH, directly from human tissue. In addition, the relative simplicity, rapidity, and specificity of this method support the potential of this basic technology, with further advancement, for assisting surgical decision-making. Graphical Abstract Mass spectrometry based profiling of hormones in human pituitary gland and tumor thin tissue sections.

  3. Effects of linseed and quercetin added to the diet of fattening lambs on the fatty acid profile and lipid antioxidant status of meat samples.

    Science.gov (United States)

    Andrés, S; Morán, L; Aldai, N; Tejido, M L; Prieto, N; Bodas, R; Giráldez, F J

    2014-06-01

    Thirty-two Merino lambs fed barley straw and a concentrate formulated either with palm oil (CTRL group) or with linseed (+LS group), both alone or supplemented with quercetin (+QCT group or +LS+QCT group) were used to assess the effects of these dietary supplements on meat quality attributes. After being slaughtered, the longissimus thoracis muscles were used to study the fatty acid (FA) profile in detail, whilst longissimus lumborum slices were stored under refrigerated conditions to determine the lipid stability. Linseed increased the content of highly unsaturated n-3 long-chain fatty acid (20:5n-3; 22:5n-3; 22:6n-3). Interestingly, a significant increment of rumenic acid content (9c,11t-18:2) was observed when this seed was administered together with dietary quercetin. Moreover, the feeding of quercetin resulted in a reduction in the proportion of saturated FA and a decrease in lipid peroxidation of meat when the lambs were fed linseed. In conclusion, from both a nutritional and a commercial (shelf-life) point of view, it may be useful to include a source of quercetin when lambs are fed linseed diets. Copyright © 2014 Elsevier Ltd. All rights reserved.

  4. 16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice

    OpenAIRE

    Walker, Alan W.; Martin, Jennifer C.; Scott, Paul; Parkhill, Julian; Flint, Harry J.; Scott, Karen P.

    2015-01-01

    Background Characterisation of the bacterial composition of the gut microbiota is increasingly carried out with a view to establish the role of different bacterial species in causation or prevention of disease. It is thus essential that the methods used to determine the microbial composition are robust. Here, several widely used molecular techniques were compared to establish the optimal methods to assess the bacterial composition in faecal samples from babies, before weaning. Results The bac...

  5. Microextraction by Packed Sorbent (MEPS) and Solid-Phase Microextraction (SPME) as Sample Preparation Procedures for the Metabolomic Profiling of Urine

    OpenAIRE

    Catarina Silva; Carina Cavaco; Rosa Perestrelo; Jorge Pereira; José S. Câmara

    2014-01-01

    For a long time, sample preparation was unrecognized as a critical issue in the analytical methodology, thus limiting the performance that could be achieved. However, the improvement of microextraction techniques, particularly microextraction by packed sorbent (MEPS) and solid-phase microextraction (SPME), completely modified this scenario by introducing unprecedented control over this process. Urine is a biological fluid that is very interesting for metabolomics studies, allowing human healt...

  6. The detection and antimicrobial susceptibility profile of Shigella isolates from meat and swab samples at butchers' shops in Gondar town, Northwest Ethiopia.

    Science.gov (United States)

    Garedew, Legesse; Hagos, Zenabu; Zegeye, Bidir; Addis, Zelalem

    2016-01-01

    Food borne pathogens are major causes of deaths, illnesses and billions of dollars of expenses. The burden of food borne illness is worsened by the ever increasing rate of antimicrobial resistance microbes. Shigella, a bacterial pathogen associated with food, is reported to account for higher prevalence rates of food borne illness in different settings. A cross-sectional study was conducted from February 10 to June 30, 2013, at the butcher houses of Gondar town in the Northwest of Ethiopia to assess the prevalence and antimicrobial susceptibility pattern of Shigella. Cattle raw meat and swab samples from selected critical control points, including knives, chopping boards, and the hands and noses of butchers, were collected and analyzed. The identification of Shigella was carried out using colony characteristics, the Gram reaction, and biochemical tests. Antimicrobial susceptibility testing was performed using the Kirby-Bauer disc diffusion method. The overall hygienic status of the butcher shops was also assessed using a checklist. An observational analysis revealed that the sanitary condition of the butcher shops and their premises was poor. Of 306 samples screened, 10.5% were positive for Shigella. Approximately 7.4% of meat samples and 10.2% of swab samples were contaminated with Shigella. Out of the total Shigella isolates, 90.6%, 46.9%, 18.8% and 9.4% were resistant to ampicillin, amoxicillin, ceftriaxone and tetracycline, respectively. A multidrug resistance pattern was recorded in 27.8% of the isolates. In conclusion, the safety of meat sold at Gondar butchers houses was poor. The identified Shigella isolates showed high levels of drug resistance and multidrug resistance patterns for commonly used antimicrobials in veterinary and human medicine. Practicing wise use of antimicrobials and strict sanitary interventions at different critical control points is strongly recommended, in addition to further in-depth studies to prevent unprecedented consequences from

  7. Characterization of the photosynthetic conditions and pigment profiles of the colour strains of Hypnea musciformis from field-collected and in vitro cultured samples

    Directory of Open Access Journals (Sweden)

    Daniela R. P. Fernandes

    2012-05-01

    Full Text Available Hypnea musciformis (Wulfen JV Lamour. is a species of great economic interest as it produces Κ-carrageenan and has shown biological activities against HIV and HSV viruses. This species displays different colour strains in its natural habitat, which may have implications for the biotechnological potential of the species. The aim of this study was to characterize the photosynthetic apparatus and pigment profile of three colour strains of H. musciformis (green, brown and red in their natural habitat and in culture. Chlorophyll a fluorescence of photosystem II was measured with a pulse-amplitude modulated fluorometer and pigments were quantified by spectrofluorimetry (chlorophyll a and spectrophotometry (phycobiliproteins. In the natural habitat, we detected significant differences between the colour strains for the following photochemical parameters: the green strain had a higher effective quantum yield (ΦPSII than the red strain and a higher maximum relative electron transport rate (rETRmax than the brown and red strains. Saturation irradiances were 1000 µE.m-2.s-1 (green and 500 µE.m-2.s-1 (brown and red. Concerning in vitro culture, the green strain presented the lowest ΦPSII, rETRmax, and α rETR, while the brown strain presented the highest values for these same parameters. The chlorophyll a content of the cultured green strain was the lowest. The phycoerythrin contents of the three colour strains were unchanged by either natural of in vitro conditions: lower in green, intermediate in brown and higher in the red strain, ensuring the chromatic identity of the strains. Our results suggest that the green strain has a better performance when exposed to high irradiance, but a lower efficiency under low irradiance compared to the brown and red strains.

  8. Characterization of the photosynthetic conditions and pigment profiles of the colour strains of Hypnea musciformis from field-collected and in vitro cultured samples

    Directory of Open Access Journals (Sweden)

    Daniela R. P. Fernandes

    2012-08-01

    Full Text Available Hypnea musciformis (Wulfen JV Lamour. is a species of great economic interest as it produces Κ-carrageenan and has shown biological activities against HIV and HSV viruses. This species displays different colour strains in its natural habitat, which may have implications for the biotechnological potential of the species. The aim of this study was to characterize the photosynthetic apparatus and pigment profile of three colour strains of H. musciformis (green, brown and red in their natural habitat and in culture. Chlorophyll a fluorescence of photosystem II was measured with a pulse-amplitude modulated fluorometer and pigments were quantified by spectrofluorimetry (chlorophyll a and spectrophotometry (phycobiliproteins. In the natural habitat, we detected significant differences between the colour strains for the following photochemical parameters: the green strain had a higher effective quantum yield (ΦPSII than the red strain and a higher maximum relative electron transport rate (rETRmax than the brown and red strains. Saturation irradiances were 1000 µE.m-2.s-1 (green and 500 µE.m-2.s-1 (brown and red. Concerning in vitro culture, the green strain presented the lowest ΦPSII, rETRmax, and α rETR, while the brown strain presented the highest values for these same parameters. The chlorophyll a content of the cultured green strain was the lowest. The phycoerythrin contents of the three colour strains were unchanged by either natural of in vitro conditions: lower in green, intermediate in brown and higher in the red strain, ensuring the chromatic identity of the strains. Our results suggest that the green strain has a better performance when exposed to high irradiance, but a lower efficiency under low irradiance compared to the brown and red strains.

  9. Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system.

    Directory of Open Access Journals (Sweden)

    He Sun

    Full Text Available In the transformation of multiple genes, gene fusion is an attractive alternative to other methods, including sexual crossing, re-transformation, and co-transformation, among others. The 2A peptide from the foot-and-mouth disease virus (FMDV causes the co-translational "cleavage" of polyprotein and operates in a wide variety of eukaryotic cells. LP4, a linker peptide that originates from a natural polyprotein occurring in the seed of Impatiens balsamina, can be split between the first and second amino acids in post-translational processing. LP4/2A is a hybrid linker peptide that contains the first nine amino acids of LP4 and 20 amino acids of 2A. The three linkers have been used as a suitable technique to link the expression of genes in some transgenic plants, but to date the cleavage efficiency of three linkers have not been comprehensively demonstrated in the same transformation system, especially in the staple crop. To verify the functions of 2A, LP4, and LP4/2A linker peptides in transgenic maize, six fusion protein vectors that each encoded a single open reading frame (ORF incorporating two report genes, Green Fluorescent Protein (GFP and β-glucuronidase (GUS, separated by 2A (or modified 2A, LP4 or LP4/2A were assembled to compare the cleavage efficiency of the three linkers in a maize transient expression system. The results demonstrated the more protein production and higher cleavage splicing efficiency with the polyprotein construct linked by the LP4/2A peptide than those of the polyprotein constructs linked by 2A or LP4 alone. Seven other fusion proteins that each encoded a single ORF incorporating two different genes GFP and Red Fluorecent Protein (RFP with different signal peptides were assembled to study the subcellular localization of genes linked by LP4/2A. The subcellular localization experiments suggested that both types of signal peptide, co-translational and post-translational, could lead their proteins to the target

  10. Modulation of phosducin-like protein 3 (PhLP3 levels promotes cytoskeletal remodelling in a MAPK and RhoA-dependent manner.

    Directory of Open Access Journals (Sweden)

    Nandini V L Hayes

    Full Text Available BACKGROUND: Phosducin-like protein 3 (PhLP3 forms a ternary complex with the ATP-dependent molecular chaperone CCT and its folding client tubulin. In vitro studies suggest PhLP3 plays an inhibitory role in β-tubulin folding while conversely in vivo genetic studies suggest PhLP3 is required for the correct folding of β-tubulin. We have a particular interest in the cytoskeleton, its chaperones and their role in determining cellular phenotypes associated with high level recombinant protein expression from mammalian cell expression systems. METHODOLOGY/PRINCIPAL FINDINGS: As studies into PhLP3 function have been largely carried out in non mammalian systems, we examined the effect of human PhLP3 over-expression and siRNA silencing using a single murine siRNA on both tubulin and actin systems in mammalian Chinese hamster ovary (CHO cell lines. We show that over-expression of PhLP3 promotes an imbalance of α and β tubulin subunits, microtubule disassembly and cell death. In contrast, β-actin levels are not obviously perturbed. On-the-other-hand, RNA silencing of PhLP3 increases RhoA-dependent actin filament formation and focal adhesion formation and promotes a dramatic elongated fibroblast-like change in morphology. This was accompanied by an increase in phosphorylated MAPK which has been associated with promoting focal adhesion assembly and maturation. Transient overexpression of PhLP3 in knockdown experiments rescues cells from the morphological change observed during PhLP3 silencing but mitosis is perturbed, probably reflecting a tipping back of the balance of PhLP3 levels towards the overexpression state. CONCLUSIONS: Our results support the hypothesis that PhLP3 is important for the maintenance of β-tubulin levels in mammalian cells but also that its modulation can promote actin-based cytoskeletal remodelling by a mechanism linked with MAPK phosphorylation and RhoA-dependent changes. PhLP3 levels in mammalian cells are thus finely poised and

  11. lpNet: a linear programming approach to reconstruct signal transduction networks.

    Science.gov (United States)

    Matos, Marta R A; Knapp, Bettina; Kaderali, Lars

    2015-10-01

    With the widespread availability of high-throughput experimental technologies it has become possible to study hundreds to thousands of cellular factors simultaneously, such as coding- or non-coding mRNA or protein concentrations. Still, extracting information about the underlying regulatory or signaling interactions from these data remains a difficult challenge. We present a flexible approach towards network inference based on linear programming. Our method reconstructs the interactions of factors from a combination of perturbation/non-perturbation and steady-state/time-series data. We show both on simulated and real data that our methods are able to reconstruct the underlying networks fast and efficiently, thus shedding new light on biological processes and, in particular, into disease's mechanisms of action. We have implemented the approach as an R package available through bioconductor. This R package is freely available under the Gnu Public License (GPL-3) from bioconductor.org (http://bioconductor.org/packages/release/bioc/html/lpNet.html) and is compatible with most operating systems (Windows, Linux, Mac OS) and hardware architectures. bettina.knapp@helmholtz-muenchen.de Supplementary data are available at Bioinformatics online. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

  12. Three small transiting planets around the M-dwarf host star LP 358-499

    Science.gov (United States)

    Wells, R.; Poppenhaeger, K.; Watson, C. A.

    2018-01-01

    We report on the detection of three transiting small planets around the low-mass star LP 358-499 (K2-133), using photometric data from the Kepler-K2 mission. Using multiband photometry, we determine the host star to be an early M dwarf with an age likely older than a gigayear. The three detected planets K2-133 b, c and d have orbital periods of ca. 3, 4.9 and 11 d and transit depths of ca. 700, 1000 and 2000 ppm, respectively. We also report a planetary candidate EPIC 247887989.01 with a period of 26.6 d and a depth of ca. 1000 ppm, which may be at the inner edge of the stellar habitable zone, depending on the specific host star properties. Using the transit parameters and the stellar properties, we estimate that the innermost planet may be rocky. The system is suited for follow-up observations to measure planetary masses and JWST transmission spectra of planetary atmospheres.

  13. Interaction of a model apolipoprotein, apoLp-III, with an oil-phospholipid interface.

    Science.gov (United States)

    Mirheydari, Mona; Mann, Elizabeth K; Kooijman, Edgar E

    2017-10-10

    Lipid droplets are "small" organelles that play an important role in de novo synthesis of new membrane, and steroid hormones, as well as in energy storage. The way proteins interact specifically with the oil-(phospho-)lipid monolayer interface of lipid droplets is a relatively unexplored but crucial question. Here, we use our home built liquid droplet tensiometer to mimic intracellular lipid droplets and study protein-lipid interactions at this interface. As model neutral lipid binding protein, we use apoLp-III, an amphipathic α-helix bundle protein. This domain is also found in proteins from the perilipin family and in apoE. Protein binding to the monolayer is studied by the decrease in the oil/water surface tension. Previous work used POPC (one of the major lipids found on lipid droplets) to form the phospholipid monolayer on the triolein surface. Here we expand this work by incorporating other lipids with different physico-chemical properties to study the effect of charge and lipid head-group size. This study sheds light on the affinity of this important protein domain to interact with lipids. Copyright © 2017. Published by Elsevier B.V.

  14. Evaluation of Llaima volcano activities for localization and classification of LP, VT and TR events

    Science.gov (United States)

    Dehghan Firoozabadi, Ali; Seguel, Fabian; Soto, Ismael; Guevara, David; Huenupan, Fernando; Curilem, Millaray; Franco, Luis

    2017-09-01

    Evaluation of seismic signals is one of the most important research topics on Volcanology. Volcanoes have daily activity; therefore, high speed evaluation of recorded signals is a challenge for improving the study of the natural phenomena occurring inside these natural formations. The aim of this paper is the evaluation (denoising, localization and classification) and analysis of Llaima volcano activities, one of the most actives volcanoes in South America. Different already proposed methods, such as, Butterworth, Spectral Subtraction (SS) and Wiener Filter (WF) are compared to the proposed Modified Spectral Subtraction (MSS) and Modified Wiener Filter (MWF) to find the best method for denoising the volcano signals. Then, event localization based on received signals of volcano is performed. In this step, Time Delay Estimation (TDE)-based method is used on data acquired from 3 mechanical sensors located in the volcano area. The proposed method is used to estimate the area for event location. The proposed denoising methods make the starting point for the event more evident to increase the localization accuracy for events where the starting point is difficult to find. In the last step, a method based on the novel DNN technique is proposed to classify the three main events occurring in the Llaima volcano (TR (Tremor), LP (Long Period) and VT (Volcano Tectonic)).

  15. Soil fertilization leads to a decline in between-samples variability of microbial community δ13C profiles in a grassland fertilization experiment.

    Directory of Open Access Journals (Sweden)

    Stavros D Veresoglou

    Full Text Available Gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS was used to measure the (13C/(12C ratios of PLFAs at natural abundance levels from a temperate grassland nitrogen (N and phosphorus (P factorial fertilization experiment in northern Greece. In each plot two rhizosphere samples were derived centred around individual Agrostis capillaris and Prunella vulgaris plants. It was hypothesized that the isotopic signal of microbes that preferentially feed on recalcitrant litter such as fungi would be modified by fertilization more strongly than that of opportunistic microbes using labile C. Microbial community δ(13C was affected by both P and N fertilization regime and plant species identity. However, we have been unable to detect significant nutrient effects on individual groups of microbes when analyzed separately in contrast to our original hypothesis. Intra-treatment variability, as evaluated from Hartley's F(max tests in the five first PCA components axes as well as the size of the convex hulls in PCA scoreplots and Mahalanobis distances, was considerably higher in the non-fertilized controls. Moreover, a significant relationship was established between the change in PLFA abundances and their respective changes in δ(13C for the aggregate of samples and those simultaneously fertilized with N and P. We conclude that use of compound specific isotope analysis in the absence of labelling represents a valuable and overlooked tool in obtaining an insight of microbial community functioning.

  16. The Toggle Local Planner for sampling-based motion planning

    KAUST Repository

    Denny, Jory

    2012-05-01

    Sampling-based solutions to the motion planning problem, such as the probabilistic roadmap method (PRM), have become commonplace in robotics applications. These solutions are the norm as the dimensionality of the planning space grows, i.e., d > 5. An important primitive of these methods is the local planner, which is used for validation of simple paths between two configurations. The most common is the straight-line local planner which interpolates along the straight line between the two configurations. In this paper, we introduce a new local planner, Toggle Local Planner (Toggle LP), which extends local planning to a two-dimensional subspace of the overall planning space. If no path exists between the two configurations in the subspace, then Toggle LP is guaranteed to correctly return false. Intuitively, more connections could be found by Toggle LP than by the straight-line planner, resulting in better connected roadmaps. As shown in our results, this is the case, and additionally, the extra cost, in terms of time or storage, for Toggle LP is minimal. Additionally, our experimental analysis of the planner shows the benefit for a wide array of robots, with DOF as high as 70. © 2012 IEEE.

  17. Influence of fruit drinks with or without lactobacillus Lp299v on the gastrointestinal uptake of paracetamol in man

    DEFF Research Database (Denmark)

    Akerman, Ulrika; Edvinsson, Lars

    2009-01-01

    BACKGROUND: Clinical observations have revealed that patients throw up undigested paracetamol tablets several hours following intake of rosehip drink with Lp299v (Proviva). The purpose of this study was to demonstrate if this translates into altered plasma levels of paracetamol in nineteen healthy...... subjects consuming 200 ml of water, Rose hip drink or Proviva together with 1.5 gram of paracetamol. FINDINGS: The concentration of paracetamol in plasma increased rapidly when the paracetamol-containing tablets were consumed together with water and after 30 minutes a median level of 90 mumol/l was reached...... (a 95% confidence interval of 57,161). The corresponding 30 minutes values of paracetamol levels in the presence of rosehip with or without Lp299v were 0 mumol/l (95% confidence intervals contain only zero for both rosehip treatments). There were significant differences in AUC, maximal paracetamol...

  18. Sensitivity analysis for CORSOR models simulating fission product release in LOFT-LP-FP-2 severe accident experiment

    Energy Technology Data Exchange (ETDEWEB)

    Hoseyni, Seyed Mohsen [Islamic Azad Univ., Tehran (Iran, Islamic Republic of). Dept. of Basic Sciences; Islamic Azad Univ., Tehran (Iran, Islamic Republic of). Young Researchers and Elite Club; Pourgol-Mohammad, Mohammad [Sahand Univ. of Technology, Tabriz (Iran, Islamic Republic of). Dept. of Mechanical Engineering; Yousefpour, Faramarz [Nuclear Science and Technology Research Institute, Tehran (Iran, Islamic Republic of)

    2017-03-15

    This paper deals with simulation, sensitivity and uncertainty analysis of LP-FP-2 experiment of LOFT test facility. The test facility simulates the major components and system response of a pressurized water reactor during a LOCA. MELCOR code is used for predicting the fission product release from the core fuel elements in LOFT LP-FP-2 experiment. Moreover, sensitivity and uncertainty analysis is performed for different CORSOR models simulating release of fission products in severe accident calculations for nuclear power plants. The calculated values for the fission product release are compared under different modeling options to the experimental data available from the experiment. In conclusion, the performance of 8 CORSOR modeling options is assessed for available modeling alternatives in the code structure.

  19. Strategic planning for minimizing CO2 emissions using LP model based on forecasted energy demand by PSO Algorithm and ANN

    Energy Technology Data Exchange (ETDEWEB)

    Yousefi, M.; Omid, M.; Rafiee, Sh. [Department of Agricultural Machinery Engineering, University of Tehran, Karaj (Iran, Islamic Republic of); Ghaderi, S.F. [Department of Industrial Engineering, University of Tehran, Tehran (Iran, Islamic Republic of)

    2013-07-01

    Iran's primary energy consumption (PEC) was modeled as a linear function of five socioeconomic and meteorological explanatory variables using particle swarm optimization (PSO) and artificial neural networks (ANNs) techniques. Results revealed that ANN outperforms PSO model to predict test data. However, PSO technique is simple and provided us with a closed form expression to forecast PEC. Energy demand was forecasted by PSO and ANN using represented scenario. Finally, adapting about 10% renewable energy revealed that based on the developed linear programming (LP) model under minimum CO2 emissions, Iran will emit about 2520 million metric tons CO2 in 2025. The LP model indicated that maximum possible development of hydropower, geothermal and wind energy resources will satisfy the aim of minimization of CO2 emissions. Therefore, the main strategic policy in order to reduce CO2 emissions would be exploitation of these resources.

  20. Conic sampling: an efficient method for solving linear and quadratic programming by randomly linking constraints within the interior.

    Science.gov (United States)

    Serang, Oliver

    2012-01-01

    Linear programming (LP) problems are commonly used in analysis and resource allocation, frequently surfacing as approximations to more difficult problems. Existing approaches to LP have been dominated by a small group of methods, and randomized algorithms have not enjoyed popularity in practice. This paper introduces a novel randomized method of solving LP problems by moving along the facets and within the interior of the polytope along rays randomly sampled from the polyhedral cones defined by the bounding constraints. This conic sampling method is then applied to randomly sampled LPs, and its runtime performance is shown to compare favorably to the simplex and primal affine-scaling algorithms, especially on polytopes with certain characteristics. The conic sampling method is then adapted and applied to solve a certain quadratic program, which compute a projection onto a polytope; the proposed method is shown to outperform the proprietary software Mathematica on large, sparse QP problems constructed from mass spectometry-based proteomics.