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Sample records for production conditions razrabotka

  1. Conditions for industrial production

    DEFF Research Database (Denmark)

    Jensen, Karsten Ingerslev; Schultz, Jørgen Munthe; Brauer, H.

    1996-01-01

    The possibility of an industrial aerogel glazing production is discussed with respect to sample size, sales volume and prices. Different ways of an industrial assembling line is outlined and the total costs of a 1 square meter aerogel glazing is calculated.......The possibility of an industrial aerogel glazing production is discussed with respect to sample size, sales volume and prices. Different ways of an industrial assembling line is outlined and the total costs of a 1 square meter aerogel glazing is calculated....

  2. Substantiation of Payment Conditions for Products

    Directory of Open Access Journals (Sweden)

    I. M. Babuk

    2004-01-01

    Full Text Available The paper proposes a model that presupposes granting more favorable payment (discount conditions with the purpose to expedite payments for products. Dependence for calculation of discount rate has been deduced on the basis of information about an actual cost of debtor’s accounts receivable. The example of discount calculation depending on a maturity of debtor’s accounts receivable is given in the paper.

  3. Impact of Work Conditions on Productivity

    Directory of Open Access Journals (Sweden)

    Anton Petričević

    2011-12-01

    Full Text Available Every institution should monitor its productivity. If productivity is decreasing, particular measures should be taken. The aim of the paper was to analyse the situation in four institutions by means of questionnaires filled in by 135 respondents, and to point to the problems that were detected through questionnaires. To increase productivity, high levels of worker responsibility should be introduced within the framework of work process, taking into account work potentials of workers, quantity and quality of work. From the results obtained through this survey it was noted that an adequately motivated worker in comfortable work environment is willing to be fully devoted to work and achieve best results, thus ultimately contributing to the overall productivity. There should be a tendency to adequately reward every worker who is willing to commit to work during full working hours and to be responsible for his or her work. Every type of work can be defined by productivity function.

  4. Possibility of Coal Combustion Product Conditioning

    Science.gov (United States)

    Błaszczyński, Tomasz Z.; Król, Maciej R.

    2018-03-01

    This paper is focused on properties of materials known as green binders. They can be used to produce aluminium-siliceous concrete and binders known also as geopolymers. Comparing new ecological binders to ordinary cements we can see huge possibility of reducing amount of main greenhouse gas which is emitted to atmosphere by 3 to even 10 times depending of substrate type used to new green material production. Main ecological source of new materials obtaining possibility is to use already available products which are created in coal combustion and steel smelting process. Most of them are already used in many branches of industry. They are mostly civil engineering, chemistry or agriculture. Conducted research was based on less popular in civil engineering fly ash based on lignite combustion. Materials were examine in order to verify possibility of obtaining hardened mortars based of different factors connected with process of geopolymerization, which are temperature, amount of reaction reagent and time of heat treatment. After systematizing the matrices for the basic parameters affecting the strength of the hardened mortars, the influence of the fly ash treatment for increasing the strength was tested.

  5. Dothistroma septosporum: spore production and weather conditions

    Energy Technology Data Exchange (ETDEWEB)

    Dvorak, M.; Drapela, K.; Kankovsky, L.

    2012-11-01

    Dartmouth's septosporum, the causal agent of Dothistroma needle blight is a widespread fungus which infects more than 80 species of coniferous trees through the entire world. Spreading of the infection is strongly affected by climatic factors of each locality where it is recorded. We attempt to describe the concrete limiting climatic factors necessary for the releasing of conidia of D. septosporum and to find out the timing of its spore production within the year. For this purpose we used an automatic volumetric spore trap and an automatic meteorological station. We found that a minimum daily average temperature of 10 degree centigrade was necessary for any spore production, as well as a long period of high air humidity. The values obtained in the present study were a little bit higher than those previously published, which may arise questions about a possible changing trend of the behaviour in the development of the Dothistroma needle blight causal agent. We used autoregressive integrated moving average (ARIMA) models to predict the spore counts on the base of previous values of spore counts and dew point. For a locality from Hackerovka, the best ARIMA model was 1,0,0; and for a locality from Lanzhot, the best was 3,1,0. (Author) 19 refs.

  6. Effects of culture conditions on acetic acid production by bacteria ...

    African Journals Online (AJOL)

    SARAH

    2015-11-30

    Nov 30, 2015 ... acid under certain culture conditions similar to cocoa fermentation stress. However ... Keywords: Acetic acid bacteria, acetic acid production, Cocoa fermentation, culture conditions ..... American Society Microbiology Press, pp.

  7. Conditions and strategies for integrating ergonomics into product development

    DEFF Research Database (Denmark)

    Broberg, Ole

    1996-01-01

    Ergonomics considerations at the stage of product development in industry is a major strategy for prevention of work-related injuries and illnesses among employees at the time of manufacture of new products. This study elucidates some important conditions and possibilities for integrating...... such considerations during the product development process. Special attention is paid to the role of design and production engineers. Based on different understandings of the product development process four approaches to integration of ergonomics are outlined....

  8. Optimization of Fermentation Conditions for the Production of Bacteriocin Fermentate

    Science.gov (United States)

    2015-03-30

    FERMENTATION CONDITIONS FOR THE PRODUCTION OF BACTERIOCIN “ FERMENTATE ” by Anthony Sikes Wayne Muller and Claire Lee March 2015...From - To) October 2010 – November 2013 4. TITLE AND SUBTITLE OPTIMIZATION OF FERMENTATION CONDITIONS FOR THE PRODUCTION OF BACTERIOCIN “ FERMENTATE ...nisin and pediocin. Whey + yeast extract was the best performing whey fermentation media. The nisin producer strain Lactococcus. lactis ssp. lactis was

  9. Optimization of fermentation conditions for trehalose production by a ...

    African Journals Online (AJOL)

    user

    2012-02-16

    Feb 16, 2012 ... The culture conditions for the production of trehalose by Rhodotorula sp. strain were optimized. The optimum ... INTRODUCTION. Trehalose is a ..... Water interplay in trehalose ... In: Prescott DM (Ed.), Methods in Cell Biology.

  10. Conditional approval: Japan lowers the bar for regenerative medicine products.

    Science.gov (United States)

    Sipp, Douglas

    2015-04-02

    A new system for conditional approval of regenerative medicine products will allow products of undetermined efficacy to enter the Japanese market. The potential scientific, economic, and ethical implications of this program highlight the need for further discussion and refinement. Copyright © 2015 Elsevier Inc. All rights reserved.

  11. Effect of diverse ecological conditions on biomass production of ...

    African Journals Online (AJOL)

    Kangaroo grass native to Australia is known as the best grass to grow on different environmental and soil conditions. Biomass production of any grass is the key factor to estimate that if the grass could fulfill the animal requirements. Biomass production of kangaroo grass was estimated in this study at three growth stages on ...

  12. Optimization of fermentation conditions for red pigment production ...

    African Journals Online (AJOL)

    An extracellular pigment-producing ascomycetous filamentous fungi belonging to the genera Penicillium was obtained from soil and its optimal culture conditions investigated. The optimal culture conditions for pigment production were as follows; soluble starch 2% (670 units), peptone (880 units), pH 9.0 (900 units); ...

  13. Screening of culture condition for xylanase production by ...

    African Journals Online (AJOL)

    The study demonstrated not only the importance of the nature of the substrate in obtaining a system resistant to catabolic repression, but also the importance of the culture conditions for biosynthesis of this enzyme. T. viride showed a high potential for xylanase production under the conditions presented in these assays.

  14. Optimizing Growth Conditions for Digoxin Production in Digitalis lanata Ehrh

    Directory of Open Access Journals (Sweden)

    Herman A. van Wietmarschen

    2016-08-01

    Full Text Available Objective: Digoxin is a therapeutic cardenolide widely used to treat various heart conditions such as atrial flutter, atrial fibrillation and heart failure in both Western as well as Chinese medicine. Digoxin is extracted from cultivated Digitalis lanata Ehrh. plants, known as Mao Hua Yang Di Huang in Chinese medicine. This manuscript presents two studies that were conducted to optimize the cultivation conditions for digoxin production in the TCM Mao Hua Yan Di Huang in a greenhouse under GAP conditions.

  15. Modifying yeast tolerance to inhibitory conditions of ethanol production processes

    Directory of Open Access Journals (Sweden)

    Luis eCaspeta

    2015-11-01

    Full Text Available Saccharomyces cerevisiae strains having a broad range of substrate utilization, rapid substrate consumption and conversion to ethanol, as well as good tolerance to inhibitory conditions are ideal for cost-competitive ethanol production from lignocellulose. A major drawback to directly design S. cerevisiae tolerance to inhibitory conditions of lignocellulosic ethanol production processes is the lack of knowledge about basic aspects of its cellular signaling network in response to stress. Here we highlight the inhibitory conditions found in ethanol production processes, the targeted cellular functions, the key contributions of integrated –omics analysis to reveal cellular stress responses according to these inhibitors, and current status on design-based engineering of tolerant and efficient S. cerevisiae strains for ethanol production from lignocellulose.

  16. Modifying Yeast Tolerance to Inhibitory Conditions of Ethanol Production Processes

    DEFF Research Database (Denmark)

    Caspeta, Luis; Castillo, Tania; Nielsen, Jens

    2015-01-01

    Saccharomyces cerevisiae strains having a broad range of substrate utilization, rapid substrate consumption, and conversion to ethanol, as well as good tolerance to inhibitory conditions are ideal for cost-competitive ethanol production from lignocellulose. A major drawback to directly design S....... cerevisiae tolerance to inhibitory conditions of lignocellulosic ethanol production processes is the lack of knowledge about basic aspects of its cellular signaling network in response to stress. Here, we highlight the inhibitory conditions found in ethanol production processes, the targeted cellular...... functions, the key contributions of integrated -omics analysis to reveal cellular stress responses according to these inhibitors, and current status on design-based engineering of tolerant and efficient S. cerevisiae strains for ethanol production from lignocellulose....

  17. Determination of optimal conditions of oxytetracyclin production from streptomyces rimosus

    International Nuclear Information System (INIS)

    Zouaghi, Atef

    2007-01-01

    Streptomyces rimosus is an oxytetracycline (OTC) antibiotic producing bacteria that exhibited activities against gram positive and negative bacteria. OTC is used widely not only in medicine but also in production industry. The antibiotic production of streptomyces covers a very wide range of condition. However, antibiotic producers are particularly fastidious cultivated by proper selection of media such as carbon source. In present study we have optimised conditions of OTC production (Composition of production media, p H, shaking and temperature). The results have been shown that bran barley is the optimal media for OTC production at 28C pH5.8 at 150rpm for 5 days. For antibiotic determination, OTC was extracted with different organic solvent. Thin-layer chromatography system was used for separation and identification of OTC antibiotic. High performance liquid chromatographic (HPLC) method with ultraviolet detection for the analysis of OTC is applied to the determination of OTC purification. (Author). 24 refs

  18. Biosurfactant production using mixed cultures under non-aseptic conditions

    International Nuclear Information System (INIS)

    Vipulanandan, C.; Ghurye, G.L.; Willson, R.C.

    1994-01-01

    The use of surfactants is of increasing interest for remediation of petroleum hydrocarbons in groundwater and soil. Surfactants increase the accessibility of adsorbed hydrocarbons and mobilize immiscible petroleum hydrocarbons for treatment. Biosurfactants have the advantage of biodegradability and non-toxicity over their synthetic counterparts, and can be produced from renewable sources. In this study the production of biosurfactant from molasses was investigated in continuously stirred batch reactors. The effects of substrate concentration, yeast extract and peptone on biomass accumulation and biosurfactant production were investigated. Biosurfactant production was quantified by surface tension reduction and critical micelle dilution (CMD). Biosurfactant production was directly correlated with biomass production, and was improved with the addition of yeast extract. Centrifugation of the whole broth reduced surface tension. The performance of the biosurfactant produced from molasses under non-aseptic condition is comparable to other published results

  19. Open and continuous fermentation: products, conditions and bioprocess economy.

    Science.gov (United States)

    Li, Teng; Chen, Xiang-bin; Chen, Jin-chun; Wu, Qiong; Chen, Guo-Qiang

    2014-12-01

    Microbial fermentation is the key to industrial biotechnology. Most fermentation processes are sensitive to microbial contamination and require an energy intensive sterilization process. The majority of microbial fermentations can only be conducted over a short period of time in a batch or fed-batch culture, further increasing energy consumption and process complexity, and these factors contribute to the high costs of bio-products. In an effort to make bio-products more economically competitive, increased attention has been paid to developing open (unsterile) and continuous processes. If well conducted, continuous fermentation processes will lead to the reduced cost of industrial bio-products. To achieve cost-efficient open and continuous fermentations, the feeding of raw materials and the removal of products must be conducted in a continuous manner without the risk of contamination, even under 'open' conditions. Factors such as the stability of the biological system as a whole during long cultivations, as well as the yield and productivity of the process, are also important. Microorganisms that grow under extreme conditions such as high or low pH, high osmotic pressure, and high or low temperature, as well as under conditions of mixed culturing, cell immobilization, and solid state cultivation, are of interest for developing open and continuous fermentation processes. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Optimization of fermentation conditions for ethanol production from whey

    Energy Technology Data Exchange (ETDEWEB)

    Castillo, F J; Izaguirre, M F; Michelena, V; Moreno, B

    1982-01-01

    Optimal conditions for ethanol production in 7% whey solutions by the yeast Candida pseudotropicalis ATCC 8619 included an initial pH of 4.57 and 30 degrees. Complete fermentation of the available lactose took place without supplementary nutrients; additions of N and P salts, yeast extract, or corn steep liquor resulted in increased yeast production and lower ethanol yields. A possible correlation was observed between increases in yeast inocula and lactose utilization and ethanol production rates; 8.35 g ethanol/L was obtained within 22 hours by using a yeast inoculum of 13.9 g/L. No differences in fermentation rates or ethanol yields were observed when whole or deproteinized whey solutions were used. Concentrated whey permeates, obtained after removal of the valuable proteins from whey, can be effectively fermented for ethanol production.

  1. Optimum Conditions for Uricase Enzyme Production by Gliomastix gueg

    Directory of Open Access Journals (Sweden)

    Atalla, M. M.

    2009-01-01

    Full Text Available Nineteen strains of microorganisms were screened for uricase production. Gliomastix gueg was recognized to produce high levels of the enzyme. The optimum fermentation conditions for uricase production by Gliomastix gueg were examined. Results showed that uric acid medium was the most favorable one, the optimum temperature was at 30ºC, and incubation period required for maximum production was 8 days with aeration level at 150 rpm and at pH 8.0. Sucrose proved to be the best carbon source, uric acid was found to be the best nitrogen source. Both, dipotassium hydrogen phosphate and ferrous chloride as well as some vitamins gave the highest amount of uricase by Gliomastix gueg.

  2. Transformation of soil and vegetable conditions at oil production territories

    Science.gov (United States)

    Gatina, Evgeniia

    2017-04-01

    On the territory of modern oil production soil, vegetation, ecosystem conditions of the environment are significantly transformed. Researches have been conducted on the oil production territories located in a boreal coniferous forest natural zone from 2005 to 2015. Standard geobotanical and soil methods are used. Mechanical destruction of a plant cover, change of the water conditions, intake of oil products and salty waters in ecosystems, pollution of the atmosphere are considered as the major technology-related factors defining transformation of land ecosystems at operation of the oil field. Under the mechanical destruction of a plant cover the pioneer plant communities are formed. These communities are characterized by most reduced specific wealth with prevalence of types of meadow groups of plants and presence of types of wetland groups of plants. The biodiversity of biocenosis which are affected linear infrastructure facilities of oil production territories and change of the water conditions, decreases. It is observed decrease in species wealth, simplification of structure of communities. Under the salting of soils in ecosystems there is a decrease species diversity of communities to prevalence nitrophilous and meadow plant species. At the increased content of organic substances in the soils that is a consequence of intake of oil products, is characteristic increase in specific richness of communities, introduction of types of wetland and oligotrophic groups of plants in forest communities. Influence depends on distance to an influence source. In process of removal from a source of atmospheric pollution in forest communities there is a decrease in species diversity and complication of structure of community. It is caused by introduction of types of meadow groups of plants in ecotone sites of the forest communities located near a source of influence and restoration of structural features of forest communities in process of removal from an influence source

  3. Conditions for Productive Learning in Network Learning Environments

    DEFF Research Database (Denmark)

    Ponti, M.; Dirckinck-Holmfeld, Lone; Lindström, B.

    2004-01-01

    are designed without a deep understanding of the pedagogical, communicative and collaborative conditions embedded in networked learning. Despite the existence of good theoretical views pointing to a social understanding of learning, rather than a traditional individualistic and information processing approach......The Kaleidoscope1 Jointly Executed Integrating Research Project (JEIRP) on Conditions for Productive Networked Learning Environments is developing and elaborating conceptual understandings of Computer Supported Collaborative Learning (CSCL) emphasizing the use of cross-cultural comparative......: Pedagogical design and the dialectics of the digital artefacts, the concept of collaboration, ethics/trust, identity and the role of scaffolding of networked learning environments.   The JEIRP is motivated by the fact that many networked learning environments in various European educational settings...

  4. Ceramic production during changing environmental/climatic conditions

    Science.gov (United States)

    Oestreich, Daniela B.; Glasmacher, Ulrich A.

    2015-04-01

    Ceramics, with regard to their status as largely everlasting everyday object as well as on the basis of their chronological sensitivity, reflect despite their simplicity the technological level of a culture and therefore also, directly or indirectly, the adaptability of a culture with respect to environmental and/or climatic changes. For that reason the question arises, if it is possible to identify changes in production techniques and raw material sources for ceramic production, as a response to environmental change, e.g. climate change. This paper will present results of a research about Paracas Culture (800 - 200 BC), southern Peru. Through several investigations (e.g. Schittek et al., 2014; Eitel and Mächtle, 2009) it is well known that during Paracas period changes in climate and environmental conditions take place. As a consequence, settlement patterns shifted several times through the various stages of Paracas time. Ceramics from three different sites (Jauranga, Cutamalla, Collanco) and temporal phases of the Paracas period are detailed archaeometric, geochemical and mineralogical characterized, e.g. Raman spectroscopy, XRD, and ICP-MS analyses. The aim of this research is to resolve potential differences in the chemical composition of the Paracas ceramics in space and time and to compare the data with the data sets of pre-Columbian environmental conditions. Thus influences of changing environmental conditions on human societies and their cultural conditions will be discussed. References Eitel, B. and Mächtle, B. 2009. Man and Environment in the eastern Atacama Desert (Southern Peru): Holocene climate changes and their impact on pre-Columbian cultures. In: Reindel, M. & Wagner, G. A. (eds.) New Technologies for Archaeology. Berlin Heidelberg: Springer-Verlag. Schittek, K., Mächtle, B., Schäbitz, F., Forbriger, M., Wennrich, V., Reindel, M., and Eitel, B.. Holocene environmental changes in the highlands of the southern Peruvian Andes (14° S) and their

  5. A Conditional Fourier-Feynman Transform and Conditional Convolution Product with Change of Scales on a Function Space II

    Directory of Open Access Journals (Sweden)

    Dong Hyun Cho

    2017-01-01

    Full Text Available Using a simple formula for conditional expectations over continuous paths, we will evaluate conditional expectations which are types of analytic conditional Fourier-Feynman transforms and conditional convolution products of generalized cylinder functions and the functions in a Banach algebra which is the space of generalized Fourier transforms of the measures on the Borel class of L2[0,T]. We will then investigate their relationships. Particularly, we prove that the conditional transform of the conditional convolution product can be expressed by the product of the conditional transforms of each function. Finally we will establish change of scale formulas for the conditional transforms and the conditional convolution products. In these evaluation formulas and change of scale formulas, we use multivariate normal distributions so that the conditioning function does not contain present positions of the paths.

  6. Biological methane production under putative Enceladus-like conditions.

    Science.gov (United States)

    Taubner, Ruth-Sophie; Pappenreiter, Patricia; Zwicker, Jennifer; Smrzka, Daniel; Pruckner, Christian; Kolar, Philipp; Bernacchi, Sébastien; Seifert, Arne H; Krajete, Alexander; Bach, Wolfgang; Peckmann, Jörn; Paulik, Christian; Firneis, Maria G; Schleper, Christa; Rittmann, Simon K-M R

    2018-02-27

    The detection of silica-rich dust particles, as an indication for ongoing hydrothermal activity, and the presence of water and organic molecules in the plume of Enceladus, have made Saturn's icy moon a hot spot in the search for potential extraterrestrial life. Methanogenic archaea are among the organisms that could potentially thrive under the predicted conditions on Enceladus, considering that both molecular hydrogen (H 2 ) and methane (CH 4 ) have been detected in the plume. Here we show that a methanogenic archaeon, Methanothermococcus okinawensis, can produce CH 4 under physicochemical conditions extrapolated for Enceladus. Up to 72% carbon dioxide to CH 4 conversion is reached at 50 bar in the presence of potential inhibitors. Furthermore, kinetic and thermodynamic computations of low-temperature serpentinization indicate that there may be sufficient H 2 gas production to serve as a substrate for CH 4 production on Enceladus. We conclude that some of the CH 4 detected in the plume of Enceladus might, in principle, be produced by methanogens.

  7. Soil physical conditions in Nigerian savannas and biomass production

    International Nuclear Information System (INIS)

    Salako, F.K.

    2004-01-01

    posed by the vast area of upland soils which are made up of coarse-textured soils and in some cases gravel and stones. Aggregates of such soils are weak, they loose productivity fast and do not retain adequate water and nutrients for sustainable production. These characteristics imply that even with the best of soil fertility amendments, soil physical conditions must be managed to achieve sustainable crop production. Plant growth had to be encouraged in the soils, such that enough biomass is produced for food and soil management. Another area which requires attention in the tropics is with regard adaptability of equipment for accurate evaluation of soil physical properties. Most commercially available equipment in the field of soil physics needs to be modified to suit the tropical environment

  8. Ordering Strategy for Fresh Agricultural Products in External Financing Condition

    Institute of Scientific and Technical Information of China (English)

    Wenyi; DU

    2014-01-01

    This paper firstly introduced the two stage supply chain consisting of single agricultural product producer and fund restraint retailer.Then,it analyzed the influence of bank interest rate on order quantity,wholesale price and expected profit of retailer and producer on the condition of retailer taking external loan strategy.Studies have shown that when the bank interest rate is in(0,0.9),the order quantity of agricultural products is a decreasing function of bank interest rate; when the bank interest rate is in(0,1),wholesale price of agricultural products decreases with increase in the bank interest rate; when the bank interest rate is in(0,0.6),the expected profit of retailer is a decreasing function of bank interest rate; when the bank interest rate is in(0,1),the expected profit of producer decreases with increase in the bank interest rate.Finally,through simulation calculation examples,it verified suitability of the conclusion,in the hope of providing reference and application value for management of supply chain.

  9. Effect of fermentation conditions on lipase production by Candida utilis

    Directory of Open Access Journals (Sweden)

    SANJA Z. GRBAVCIC

    2007-08-01

    Full Text Available A wild yeast strain isolated from spoiled soybean oil and identified as Candida utilis initially presented rather low lipase activity (approximately 4 IU dm-3 in submerged culture in a universal yeast medium containing 2 % malt extract. Stu­dies were undertaken to improve the lipase production. The best yields of lipase were obtained with a medium supplemented with caprylic and oleic acids as indu­cers, but higher concentrations of the former (> 0.5 % had a negative effect on the lipase production and cell growth. The type of nitrogen source seemed also to be very important. The highest lipolytic activity of 284 IU dm-3 was achieved after 5 days of fermentation in a medium containing oleic acid and hydrolyzed casein as carbon and nitrogen sources, respectively, and supplemented with Tween 80®. It was shown that optimization of the fermentation conditions can lead to a significant improvement in the lipase production (more than 70-fold higher compared to the initial value obtained in the non-optimized medium.

  10. PRODUCTIVITY OF MICROALGAE CHLORELLA VULGARIS IN LABORATORY CONDITION

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    Agnieszka Patyna

    2017-06-01

    Full Text Available Algae biomass is increasingly regarded as a potential resource that could be used to produce biofuels, electricity and heat. Algae contain a lot of nutrients, so they can be used as food for humans and livestock. Because of their valuable composition (many nutrients they are used as supplements of balanced diet, in turn taking into account their biosorption abbility they are used to detoxification of human body. Algae cultivation does not demand large areas of land to expose cells to sunlight, so their production rate is higher than vascular plants. Moreover algae cultivation lets to achieve high biomass concentration. Important cultivation factors are: illumination (light intensity is an important factor because it drives photosynthesis, CO2 supply, culture medium and mixing. The experimental research was conducted using Chlorella vulgaris BA 002 strain. The aim of this study was to determine the effectiveness of biomass growth in laboratory condition.

  11. Experimental observations on feasibility conditions for neutron production by fractofusion

    International Nuclear Information System (INIS)

    Shyam, A.; Kaushik, T.C.; Kulkarni, L.V.

    1998-01-01

    The feasibility conditions for production of neutrons by fractofusion have been investigated. None of the low energy/velocity experiments, such as deuterium absorption/adsorption in metals, mechanical crushing and thermal cycling of deuterides, chemical reactions with heavy water are observed to produce neutrons. High velocity (till 1 km/s) fractofusion experiments are performed by accelerating projectiles by electro-magnetic (rail) gun. These projectiles are impacted on deuterides of lithium (non-conducting) and titanium/palladium (metallic conductivity). While lithium deuteride samples are observed to produce 10 1 to 10 2 neutrons per impact, no neutrons could be observed when palladium/titanium deuterides are impacted with similar projectiles. (author)

  12. Chemical impurity production under boronized wall conditions in TEXTOR

    International Nuclear Information System (INIS)

    Philipps, V.; Vietzke, E.; Erdweg, M.

    1992-01-01

    The TEXTOR SNIFFER probe has been used to analyse the chemical impurity production under various plasma and boronized wall conditions. Methane formation has been observed to 0.6-1 x 10 -2 CH 4 /H at room temperature, increasing slightly with increasing density in the SOL. The hydrocarbon formation yields increase from R.T. to the maximum at about 500 o C by a factor of 1.5-2.5. Increasing the impact energy by biasing the graphite plate leads to a decrease of the hydrocarbon yield at room temperature but to an increase at 500 o C. Chemical CO formation due interaction of oxygen impurities with the graphite reaches ratios between 0.5 and 3 x 10 -2 CO/H,D increasing with increasing distance to the limiter edge. (author) 10 refs., 6 figs

  13. Increased root production in soybeans grown under space flight conditions.

    Science.gov (United States)

    Levine, H. G.; Piastuch, W. C.

    The GENEX ({Gen}e {Ex}pression) spaceflight experiment (flown on STS-87) was developed to investigate whether direct and/or indirect effects of microgravity are perceived as an external stimulus for soybean seedling response. Protocols were designed to optimize root and shoot formation, gas exchange and moisture uniformity. Six surface sterilized soybean seeds (Glycine max cv McCall) were inserted into each of 32 autoclaved plastic seed growth pouches containing an inner germination paper sleeve (for a total of 192 seeds). The pouches were stowed within a mid-deck locker until Mission Flight Day 10, at which time an astronaut added water to each pouch (thereby initiating the process of seed germination on-orbit), and subsequently transferred them to four passive, light-tight aluminum canisters called BRIC-60s (Biological Research In Canisters). We report here on the morphological characteristics of: (1) the recovered flight material, (2) the corresponding ground control population, plus (3) additional controls grown on the ground under clinostat conditions. No significant growth differences were found between the flight, ground control and clinorotated treatments for either the cotyledons or hypocotyls. There were, however, significantly longer primary roots produced in the flight population relative to the ground control population, which in turn had significantly longer primary roots than the clinorotated population. This same pattern was observed relative to the production of lateral roots (flight > control > clinorotated). Taken together with previous literature reports, we believe that there is now sufficient evidence to conclude that plants grown under conditions of microgravity will generally exhibit enhanced root production relative to their ground control counterparts. The mechanism underlying this phenomenon is open to speculation. Funded under NASA Contract NAS10-12180.

  14. Diet and bioclimatic conditions on production and milk quality

    Directory of Open Access Journals (Sweden)

    F. P. Campos

    2018-05-01

    Full Text Available It was aimed to analyze the productive performance of lactating cows on isoprotein fed diets, at differentiated environmental conditions. Eight Holstein cows were used, grouped in two 4 × 4 balanced Latin squares design. The treatments were evaluated in 2 × 2 factorial designs: sources of roughage (corn silage – CS plus concentrate, and the combination of corn silage with sugarcane - CSSC, 1:1 on DM, plus concentrate and distinct environment (with= WS and without= OS, fan and nebulizers system= Sfn. The estimation of dry matter intake (DMI, productive performance and physicochemical parameters of milk were evaluated. There was no interaction effect of environment factors and source of forage. There was signiÀ cant effects for the source forage factors, where the DMI for the CSSC based diet was higher than the CS based diet in the effect of forage (4.22 vs. 4.06% BW, and 22.3 vs. 21.7 kg/d, respectively, P≤0.05, but with similar milk production correcting 3.5% fat (23.01 vs. 22.62, CSSC and CS; 22.85 vs. 22.78 kg/day, WS and OS, respectively, P≥0.05. The feed efÀ ciency and conversion was similar in both factors (102.8 vs. 104.7% and 0.99 vs. 1.0, CSSC and CS; 102.5 vs. 104.9% and 1.0 vs. 0.99, WS and OS, respectively, P≥0.05. The beneÀ t-diet cost ratio was higher for CS-based diet than for CSSC (7.44 vs. 6.97, P≤0.05. There were effects only in the forage factor for CP milk (3.26 vs. 3.23%, P≤0.05, lactose (4.54 vs. 4.49%, P≤0.05 and urea nitrogen in milk (23.21 vs. 20.71 mg/dL, P≤0.05 and the superiority arising from the CSSC-based diet in comparison to the CS diet. There was higher for T and THI (28.1 vs. 23.6°C and 75.1 vs. 71.1, respectively, P≤0.05, and lower RH to 2:00 pm (47.7 vs. 64.5%, P≤0.05. The linear score showed negative correlations with DMI, milk production, lactose and urea nitrogen (-0.36, -0.69, -0.44 and -0.32, P≤0.05, respectively. The use of the diet based on CSSC proposed increases in DMI and

  15. Determination of the Optimum Conditions for Production of Chitosan Nanoparticles

    Directory of Open Access Journals (Sweden)

    A. Dustgani

    2007-12-01

    Full Text Available Bioedegradable nanoparticles are intensively investigated for their potential applications in drug delivery systems. Being a biocompatible and biodegradable polymer, chitosan holds great promise for use in this area. This investigation was concerned with determination and optimization of the effective parameters involved in the production of chitosan nanoparticles using ionic gelation method. Studied variables were concentration and pH of the chitosan solution, the ratio of chitosan to sodium tripolyphosphate therein and the molecular weight of chitosan. For this purpose, Taguchistatistical method was used for design of experiments in three levels. The size of chitosan nanoparticle was determined using laser light scattering. The experimental results showed that concentration of chitosan solution was the most important parameter and chitosan molecular weight the least effective parameter. The optimum conditions for preparation of nanoparticles were found to be 1 mg/mL chitosan solution with pH=5, chitosan to sodium tripolyphosphate ratio of 3 and chitosan molecular weight of 200,000 daltons. The average nanoparticle size at optimum conditions was found to be about 150 nm.

  16. Optimal operating conditions for maximum biogas production in anaerobic bioreactors

    International Nuclear Information System (INIS)

    Balmant, W.; Oliveira, B.H.; Mitchell, D.A.; Vargas, J.V.C.; Ordonez, J.C.

    2014-01-01

    The objective of this paper is to demonstrate the existence of optimal residence time and substrate inlet mass flow rate for maximum methane production through numerical simulations performed with a general transient mathematical model of an anaerobic biodigester introduced in this study. It is herein suggested a simplified model with only the most important reaction steps which are carried out by a single type of microorganisms following Monod kinetics. The mathematical model was developed for a well mixed reactor (CSTR – Continuous Stirred-Tank Reactor), considering three main reaction steps: acidogenesis, with a μ max of 8.64 day −1 and a K S of 250 mg/L, acetogenesis, with a μ max of 2.64 day −1 and a K S of 32 mg/L, and methanogenesis, with a μ max of 1.392 day −1 and a K S of 100 mg/L. The yield coefficients were 0.1-g-dry-cells/g-pollymeric compound for acidogenesis, 0.1-g-dry-cells/g-propionic acid and 0.1-g-dry-cells/g-butyric acid for acetogenesis and 0.1 g-dry-cells/g-acetic acid for methanogenesis. The model describes both the transient and the steady-state regime for several different biodigester design and operating conditions. After model experimental validation, a parametric analysis was performed. It was found that biogas production is strongly dependent on the input polymeric substrate and fermentable monomer concentrations, but fairly independent of the input propionic, acetic and butyric acid concentrations. An optimisation study was then conducted and optimal residence time and substrate inlet mass flow rate were found for maximum methane production. The optima found were very sharp, showing a sudden drop of methane mass flow rate variation from the observed maximum to zero, within a 20% range around the optimal operating parameters, which stresses the importance of their identification, no matter how complex the actual bioreactor design may be. The model is therefore expected to be a useful tool for simulation, design, control and

  17. Fuel behavior and fission product release under HTGR accident conditions

    International Nuclear Information System (INIS)

    Fukuda, K.; Hayashi, K.; Shiba, K.

    1990-01-01

    In early 1989 a final decision was made over construction of a 30 MWth HTGR called the High Temperature Engineering Test Reactor, HTTR, in Japan in order to utilize it for high temperature gas engineering tests and various nuclear material tests. The HTTR fuel is a pin-in-block type fuel element which is composed of a hexagonal graphite block with dimension of 580 mm in length and 360 mm in face-to-face distance and about 30 of the fuel rods inserted into the coolant channels drilled in the block. The TRISO coated fuel particles for HTTR are incorporated with graphite powder and phenol resin into the fuel compacts, 19 of which are encased into a graphite sleeve as a fuel rod. It is necessary for the HTTR licensing to prove the fuel stability under predicted accidents related to the high temperature events. Therefore, the release of the fission products and the fuel failure have been investigated in the irradiation---and the heating experiments simulating these conditions at JAERI. This report describes the HTTR fuel behavior at extreme temperature, made clear in these experiments

  18. Mixotrophic transition induced lipid productivity in Chlorella pyrenoidosa under stress conditions for biodiesel production

    Directory of Open Access Journals (Sweden)

    Hari Prasad Ratnapuram

    2018-01-01

    Full Text Available Influence of mixotrophic mode and its transition to various trophic modes under stress conditions was assessed during two stage cultivation of Chlorella pyrenoidosa. Significant lipid productivity was triggered under low light intensity, glucose + bicarbonate supplementation and nitrogen starvation. The association between biomass and lipid productivity, fatty acid composition during mixotrophic transition was critically evaluated. Biomass in growth phase (GP and stress phase (SP was 6.14 g/l and 5.14 g/l, respectively, in mixotrophic mode. Higher lipid productivity of 284 g/kg and 154.3 g/kg of neutral lipids was achieved in SP in mixotrophic-mixotrophic (MM and mixotrophic-heterotrophic (MH modes, respectively. Stress conditions resulted in high unsaturated fatty acid methyl esters in MH mode. In addition, neutral lipid content was 58% in MH and 52% in MM, that can be attributed to carbon source that is supplemented even in stress phase. Exploring such novel strategies can generate sustainable avenues for biodiesel production.

  19. Selection of culture medium and conditions for the production of ...

    African Journals Online (AJOL)

    oyaide

    2013-05-15

    May 15, 2013 ... improving the productivity and economical benefits in livestock production ... was to improve the yeast biomass production measured as dry cell ... the total livestock population in India was 1708 Million ... Media for culture maintenance and optimization .... which is very economical and efficient source for the.

  20. Chemistry of fission product iodine under nuclear reactor accident conditions

    International Nuclear Information System (INIS)

    Malinauskas, A.P.; Bell, J.T.

    1986-01-01

    The radioisotopes of iodine are generally acknowledged to be the species whose release into the biosphere as a result of a nuclear reactor accident is of the greatest concern. In the course of its release, the fission product is subjected to differing chemical environments; these can alter the physicochemical form of the fission product and thus modify the manner and extent to which release occurs. Both the chemical environments which are characteristic of reactor accidents and their effect in determining physical and chemical form of fission product iodine have been studied extensively, and are reviewed in this report. 76 refs

  1. 75 FR 57019 - Pesticide Product Registrations; Conditional Approval

    Science.gov (United States)

    2010-09-17

    ..., the Agency was able to make basic health and safety determinations which show that use of flutriafol... unreasonable adverse effects to man and the environment. III. Conditional Approval Form EPA issued a notice...

  2. 77 FR 10515 - Pesticide Product Registrations; Conditional Approval

    Science.gov (United States)

    2012-02-22

    ... reviews, the Agency was able to make basic health and safety determinations which show that use of HeiQ... unreasonable adverse effects to man and the environment. The conditions of this registration can be reviewed in...

  3. Effects of nutritional conditions on lipid production by cyanobacteria.

    Science.gov (United States)

    Cordeiro, Raquel S; Vaz, Izabela C D; Magalhães, Sérgia M S; Barbosa, Francisco A R

    2017-01-01

    The present study evaluated the effects of the culturing media and the levels of nitrogen and phosphorus on the growth, biomass productivity and lipid production of four species of Microcystis (M. novacekii, M. aeruginosa, M panniformis and M. protocystis). The lipid extract was obtained by refluxing with dichloromethane (Soxhlet). The biomass and biomass productivity yields were maximized with ASM-1 medium treatment enriched with nitrogen and/or phosphorus (0.25-0.65 g/L and 25-50.7 mg/L d-1, respectively). The lipid extract yields from M. panniformis and M. novacekii were inversely correlated with the concentration of nitrogen and directly correlated with the concentration of phosphorus (35.8 % and 31.7 %). The lipid extract yield from M. aeruginosa was inversely correlated with the nutrient concentration (23.3 %). M. protocystis exhibited a higher lipid content in the control medium (41.5 %) than in the nitrogen-enriched media. The recorded results show that a nutrient-poor culture medium favours cell growth and stimulates lipid accumulation, which directly affects the cost of cultivation by reducing nutrient consumption. All studied species may serve as biomass sources for biodiesel production, although M. protocystis exhibited the highest lipid production. Further studies are necessary to determine the composition of the recorded lipid extract.

  4. Effects of nutritional conditions on lipid production by cyanobacteria

    Directory of Open Access Journals (Sweden)

    RAQUEL S. CORDEIRO

    Full Text Available ABSTRACT The present study evaluated the effects of the culturing media and the levels of nitrogen and phosphorus on the growth, biomass productivity and lipid production of four species of Microcystis (M. novacekii, M. aeruginosa, M panniformis and M. protocystis. The lipid extract was obtained by refluxing with dichloromethane (Soxhlet. The biomass and biomass productivity yields were maximized with ASM-1 medium treatment enriched with nitrogen and/or phosphorus (0.25-0.65 g/L and 25-50.7 mg/L d-1, respectively. The lipid extract yields from M. panniformis and M. novacekii were inversely correlated with the concentration of nitrogen and directly correlated with the concentration of phosphorus (35.8 % and 31.7 %. The lipid extract yield from M. aeruginosa was inversely correlated with the nutrient concentration (23.3 %. M. protocystis exhibited a higher lipid content in the control medium (41.5 % than in the nitrogen-enriched media. The recorded results show that a nutrient-poor culture medium favours cell growth and stimulates lipid accumulation, which directly affects the cost of cultivation by reducing nutrient consumption. All studied species may serve as biomass sources for biodiesel production, although M. protocystis exhibited the highest lipid production. Further studies are necessary to determine the composition of the recorded lipid extract.

  5. Effects of cultivation medium and conditions on the production of ...

    African Journals Online (AJOL)

    ... synthetic carbon source for the production of α-gal for Aspergillus awamori and Aspergillus carbonarius respectively; L-Asparagine (0.262 %) was the best concentration of nitrogen source for Aspergillus awamori whereas sodium nitrate (0.4 %) was the best concentration of nitrogen source for Aspergillus carbonarius and ...

  6. Culture conditions for the production of a tannase of Aspergillus ...

    African Journals Online (AJOL)

    This fungus produced tannase in a fermentation medium M containing tannic acid as the only carbon source. Time course of enzyme synthesis by the fungus showed that the enzyme production followed logarithmic growth phase with maximum enzyme yield being obtained after 6 days corresponding to the culture pH of 3.8.

  7. Effects of fermentation conditions on the production of 4-α ...

    African Journals Online (AJOL)

    ajl yemi

    2011-11-30

    Nov 30, 2011 ... studied the recombinant protein of human IGF-1 in rich and minimal ... recombinant protein A-β-lactamase compared to the medium pH at 7.0. ... shake flask fermentation and provided desired conditions for fermentation in 5 L ..... expression kinetics study in bioreactor, which would help to enhance cell ...

  8. Feasibility of winter cover crop production under rainfed conditions

    African Journals Online (AJOL)

    ACSS

    CONDITIONS IN THE EASTERN CAPE PROVINCE OF SOUTH AFRICA. L. MUZANGWA, C. ... planting, resulting in higher weed dry weights at 3 and 6 weeks after planting (WAP). April planted cover crops ...... of micro-arthropods in a sub-tropical forest ecosystem ... American Association of Cereal Chemists, Inc. St. Paul ...

  9. Production of extracellular chitinase Beauveria bassiana under submerged fermentation conditions

    Science.gov (United States)

    Elawati, N. E.; Pujiyanto, S.; Kusdiyantini, E.

    2018-05-01

    Chitinase-producing microbes have attracted attention as one of the potential agents for control of phytopathogenic fungi and insect pests. The fungus that potentially produces chitinase is Beauveria bassiana. This study aims to determine the growth curve and chitinase activities of B. bassiana isolated from Helopeltis antonii insects after application. Method of measuring growth curve was done by dry cell period method, while for measurement of enzyme activity done by measuring absorbance at spectrophotometer. The results showed optimum growth time of B. bassiana with the highest cell count of 0.031 g on day 4 which was log phase, while the highest enzyme activity was 0,585 U / mL on the 4th day for 7 days incubation. Based on these results when correlated growth with enzyme production, chitinase enzyme products are produced in log phase and categorized as primary metabolism.

  10. Predictive Models for Photovoltaic Electricity Production in Hot Weather Conditions

    Directory of Open Access Journals (Sweden)

    Jabar H. Yousif

    2017-07-01

    Full Text Available The process of finding a correct forecast equation for photovoltaic electricity production from renewable sources is an important matter, since knowing the factors affecting the increase in the proportion of renewable energy production and reducing the cost of the product has economic and scientific benefits. This paper proposes a mathematical model for forecasting energy production in photovoltaic (PV panels based on a self-organizing feature map (SOFM model. The proposed model is compared with other models, including the multi-layer perceptron (MLP and support vector machine (SVM models. Moreover, a mathematical model based on a polynomial function for fitting the desired output is proposed. Different practical measurement methods are used to validate the findings of the proposed neural and mathematical models such as mean square error (MSE, mean absolute error (MAE, correlation (R, and coefficient of determination (R2. The proposed SOFM model achieved a final MSE of 0.0007 in the training phase and 0.0005 in the cross-validation phase. In contrast, the SVM model resulted in a small MSE value equal to 0.0058, while the MLP model achieved a final MSE of 0.026 with a correlation coefficient of 0.9989, which indicates a strong relationship between input and output variables. The proposed SOFM model closely fits the desired results based on the R2 value, which is equal to 0.9555. Finally, the comparison results of MAE for the three models show that the SOFM model achieved a best result of 0.36156, whereas the SVM and MLP models yielded 4.53761 and 3.63927, respectively. A small MAE value indicates that the output of the SOFM model closely fits the actual results and predicts the desired output.

  11. [Tablets and tablet production - with special reference to Icelandic conditions].

    Science.gov (United States)

    Skaftason, Jóhannes F; Jóhannesson, Thorkell

    2013-04-01

    Modern tablet compression was instituted in England in 1844 by William Brockedon (1787-1854). The first tablets made according to Brockedon´s procedures contained watersoluble salts and were most likely compressed without expedients. In USA a watershed occurred around 1887 when starch (amylum maydis) was introduced to disperse tablets in aqueous milieu in order to corroborate bioavailability of drugs in the almentary canal. About the same time great advances in tablet production were introduced by the British firm Burroughs Wellcome and Co. In Denmark on the other hand tablet production remained on low scale until after 1920. As Icelandic pharmacies and drug firms modelled themselves mostly upon Danish firms tablet production was first instituted in Iceland around 1930. The first tablet machines in Iceland were hand-driven. More efficent machines came after 1945. Around 1960 three sizeable tablet producers were in Iceland; now there is only one. Numbers of individual tablet species (generic and proprietary) on the market rose from less than 10 in 1913 to 500 in 1965, with wide variations in numbers in between. Tablets have not wiped out other medicinal forms for peroral use but most new peroral drugs have been marketed in the form of tablets during the last decades.

  12. Examination of Incubation Conditions for Production of HERICIUM ERINACEUM

    Science.gov (United States)

    Okumura, Ryosuke; Sasaki, Chizuru; Asada, Chikako; Nakamura, Yoshitoshi

    Basidiomycetes has recently attracted considerable attention for its various physiological activities, such as antitumor, antioxidant and immunostimulating activities. Compounds isolated from fruit body of Hericium erinaceum, commonly called Yamabushitake in Japan, have interesting biological activities such as cytotoxic effectors on cancer cell (HeLa cells) and stimulators of synthesis of nerve growth factor. It is necessary for the cultivation of the fruit body of mushroom to control light, temperature, humidity. Otherwise, mycelia cultivation needs only temperature control. H. erinaceum cultivated by submerged culture have similar physiological activities to the fruit body of H. erinaceum, which suggests cultured mycelia can potentially become a promoter of synthesis of nerve growth factor. In this study, we used whey which is by-products of cheese-making process as an alternative nitrogen source in submerged cultivation of H. erinaceum mycelia, and then dry cell weight (DCW) and DCW productivity of whey medium were compared with those of chemical nutrient medium. When whey was used as a nitrogen source, DCW and DCW productivity are 1.5 times higher than those of chemical nutrient medium, 5.99 g/L and 0.60 g/L/day, respectively. It was suggested that whey could be used as an alternative nitrogen source and a growth promoting factor in H. erinaceum mycelia cultivation.

  13. Photochemical processes and ozone production in Finnish conditions

    Energy Technology Data Exchange (ETDEWEB)

    Laurila, T.; Hakola, H. [Finnish Meteorological Inst., Helsinki (Finland). Air Quality Dept.

    1996-12-31

    Photochemical ozone production is observed in March-September. Highest ozone concentrations and production efficiencies are observed in spring in the northern parts and in summer in the southern parts of the country. VOC concentrations are relatively low compared to continental areas in general. During the growing season a substantial part of the total reactive mass of VOCs is of biogenic origin. Large forest areas absorb ozone substantially, decreasing the ambient ozone concentrations in central and northern parts of Finland where long-range transport of ozone is relatively important compared to local production. The aim of the work conducted at Finnish Meteorological Institute has been to characterise concentrations of photochemically active species in the boundary layer and their photochemical formation and deposition including the effects on vegetation. Also interactions between the boundary layer and free troposphere of ozone have been studied. In the future, fluxes of both biogenic species and air pollutants will be measured and the models will be further developed so that the photochemical and micrometeorological processes could be better understood

  14. Photochemical processes and ozone production in Finnish conditions

    Energy Technology Data Exchange (ETDEWEB)

    Laurila, T; Hakola, H [Finnish Meteorological Inst., Helsinki (Finland). Air Quality Dept.

    1997-12-31

    Photochemical ozone production is observed in March-September. Highest ozone concentrations and production efficiencies are observed in spring in the northern parts and in summer in the southern parts of the country. VOC concentrations are relatively low compared to continental areas in general. During the growing season a substantial part of the total reactive mass of VOCs is of biogenic origin. Large forest areas absorb ozone substantially, decreasing the ambient ozone concentrations in central and northern parts of Finland where long-range transport of ozone is relatively important compared to local production. The aim of the work conducted at Finnish Meteorological Institute has been to characterise concentrations of photochemically active species in the boundary layer and their photochemical formation and deposition including the effects on vegetation. Also interactions between the boundary layer and free troposphere of ozone have been studied. In the future, fluxes of both biogenic species and air pollutants will be measured and the models will be further developed so that the photochemical and micrometeorological processes could be better understood

  15. Bioethanol production from Scenedesmus obliquus sugars. The influence of photobioreactors and culture conditions on biomass production

    Energy Technology Data Exchange (ETDEWEB)

    Miranda, J.R.; Passarinho, P.C.; Gouveia, L. [Laboratorio Nacional de Energia e Geologia (LNEG), Lisbon (Portugal). Unidade de Bioenergia

    2012-10-15

    A closed-loop vertical tubular photobioreactor (PBR), specially designed to operate under conditions of scarce flat land availability and irregular solar irradiance conditions, was used to study the potential of Scenedesmus obliquus biomass/sugar production. The results obtained were compared to those from an open-raceway pond and a closed-bubble column. The influence of the type of light source and the regime (natural vs artificial and continuous vs light/dark cycles) on the growth of the microalga and the extent of the sugar accumulation was studied in both PBRs. The best type of reactor studied was a closed-loop PBR illuminated with natural light/dark cycles. In all the cases, the relationship between the nitrate depletion and the sugar accumulation was observed. The microalga Scenedesmus was cultivated for 53 days in a raceway pond (4,500 L) and accumulated a maximum sugar content of 29 % g/g. It was pre-treated for carrying out ethanol fermentation assays, and the highest ethanol concentration obtained in the hydrolysate fermented by Kluyveromyces marxianus was 11.7 g/L. (orig.)

  16. Bioethanol production from Scenedesmus obliquus sugars: the influence of photobioreactors and culture conditions on biomass production.

    Science.gov (United States)

    Miranda, J R; Passarinho, P C; Gouveia, L

    2012-10-01

    A closed-loop vertical tubular photobioreactor (PBR), specially designed to operate under conditions of scarce flat land availability and irregular solar irradiance conditions, was used to study the potential of Scenedesmus obliquus biomass/sugar production. The results obtained were compared to those from an open-raceway pond and a closed-bubble column. The influence of the type of light source and the regime (natural vs artificial and continuous vs light/dark cycles) on the growth of the microalga and the extent of the sugar accumulation was studied in both PBRs. The best type of reactor studied was a closed-loop PBR illuminated with natural light/dark cycles. In all the cases, the relationship between the nitrate depletion and the sugar accumulation was observed. The microalga Scenedesmus was cultivated for 53 days in a raceway pond (4,500 L) and accumulated a maximum sugar content of 29 % g/g. It was pre-treated for carrying out ethanol fermentation assays, and the highest ethanol concentration obtained in the hydrolysate fermented by Kluyveromyces marxianus was 11.7 g/L.

  17. Working Conditions as a Factor of Productivity in SMEs

    OpenAIRE

    Držajić , Katarina; Vega , Frank

    2017-01-01

    International audience; The theoretical framework by Diamond, Mortensen and Pissarides (Nobel Award in Economic Sciences, 2010) shows us how generous wages can determine the success in filling job vacancies. The empirical evidence is convincing: larger and more profitable enterprises pay higher wages. Nowadays, large leader companies are investing not only in paying efficient wages to prevent shirking, but also in the improvement of working conditions and even taking a rigorous analytical app...

  18. Optimisation of Shift Reactor Operating Conditions to Maximise Hydrogen Production

    Energy Technology Data Exchange (ETDEWEB)

    Sanchez, J. M.; Marano, M.; Ruiz, E.

    2011-07-28

    This report compiles the results of the work conducted by CIEMAT for Task 6.5 Shift reaction of the FLEXGAS project Near Zero Emission Advanced Fluidized Bed Gasification, which has been carried out with financial support from the Research Fund for Coal and Steel, RFCR-CT-2007-00005. The activity of an iron-chromium-based catalyst for the water gas shift reaction is studied. Results about WGS experiments conducted by CIEMAT on laboratory scale under different operating conditions are presented. The influence on the activity of the catalyst of main operating parameters- temperature, pressure, excess steam, and space velocity and gas composition - is evaluated and discussed. (Author) 19 refs.

  19. Media and growth conditions for induction of secondary metabolite production

    DEFF Research Database (Denmark)

    Frisvad, Jens Christian

    2012-01-01

    -defined media are suited for biochemical studies, but in order to get chemical diversity expressed in filamentous fungi, sources rich in amino acids, vitamins, and trace metals have to be added, such as yeast extract and oatmeal. A battery of solid agar media is recommended for exploration of chemical diversity...... as agar plug samples are easily analyzed to get an optimal representation of the qualitative secondary metabolome. Standard incubation for a week at 25°C in darkness is recommended, but optimal conditions have to be modified depending on the ecology and physiology of different filamentous fungi....

  20. PRODUCTION OF WIRE RML OF SQUARE PROFILE IN CONDITIONS OF BELORUSSIAN METALLURGICAL WORKS

    Directory of Open Access Journals (Sweden)

    D. G. Sachava

    2010-01-01

    Full Text Available It is shown that at using of profile (square wire RML in conditions of RUP «BMZ» for production of pipes of high pressure their production is cheapened, what attracts interest of consumer.

  1. Siderophore production by mycorrhizal sorghum roots under micronutrient deficient condition

    Directory of Open Access Journals (Sweden)

    N. Aliasgharzad

    2009-05-01

    Full Text Available It has widely been accepted that mycorrhizal symbiosis improves micronutrients uptake by most of the plants. In this study, sorghum (Sorghum bicolor L. plants were grown in sterile perlite and were inoculated with either Glomus etunicatum (GE or G.intraradices (GI, while the control set was left un-inoculated. Rorison's nutrient solution with three levels of 0, half and full strength (C0, C0.5 and C1, respectively of Fe, Cu, Zn and Mn was applied to the pots during 85 days of growth period. Chrome azurol-S assay was used for determination of siderophores in root leachates on 45, 65 and 85 days after sowing (DAS. Siderophore production per unit volume of root was higher in mycorrhizal than non-mycorrhizal plants. Both GE and GI were efficient fungi in this respect. Siderophore production was significantly induced at C0 level of the micronutrients. Amount of siderophores produced on 45 and 85 DAS was more than 65 DAS. Mycorrhizal root colonization by GE or GI was not significantly affected by micronutrient levels.

  2. Changed market conditions for biogas production; Foeraendrade marknadsvillkor foer biogasproduktion

    Energy Technology Data Exchange (ETDEWEB)

    Colnerud Granstroem, Sigrid; Gaaverud, Henrik; Glimhall, Alexandra

    2010-10-15

    The Swedish gas market consists mainly of the natural gas network that extends through the southwestern Sweden, and the local biogas markets. Biogas share of the Swedish gas market is growing steadily. The fact that the Swedish gas net is limited and fragmented forms an obstacle for biogas use to expand. That the gas market as a whole, natural gas included, must develop and expand is therefore a prerequisite for the large potential for Swedish Biogas to be realized. This in contrast with the ultimate objective to completely replace natural gas in the Swedish gas market. When policy changes are made in order to support biogas it is crucial for long-term competitiveness of biogas that these changes should not impact the natural gas market and hinder its development. Such a scenario would ultimately mean that also biogas development opportunities deteriorate. Biogas operations encounter three main problems that prevent or impede its expansion in the gas market. First, the potential for profitability in biogas production must be enhanced. Second, natural gas and biogas markets should be more integrated with each other. Thirdly, the biogas must be distributed in a cost-effective manner. The present investigation aims to supplement the Natural Gas Act with special provisions which takes into account the input and transmission of biogas. In addition to the production of biogas, it is now the producer's responsibility to clean the gas from water vapor, hydrogen sulfide and carbon dioxide and to augment the calorific value of the gas to the standard of Danish natural gas quality by propane addition and to ensure that the physical connection to network is available. There are thus a number of options available for shifting demarcation between biogas production and network operations. Short-term competitiveness of biogas would be strengthened most if purification and spiking the gas with propane and the connection to the network was imposed on network owners. In the

  3. Corrosion products behaviour under VVER primary coolant conditions

    International Nuclear Information System (INIS)

    Grygar, T.; Zmitko, M.

    2002-01-01

    The aim of this work was to collect data on thermodynamic stability of Cr, Fe, and Ni oxides, mechanisms of hydrothermal corrosion of stainless steels and to compare the real observation with the theory. We found that the electrochemical potential and pH in PWR and VVER are close to the thermodynamic boundary between two fields of stable spinel type oxides. The ways of degradation of the passivating layers due to changes in water chemistry were considered and PWR and VVER systems were found to be potentially endangered by reductive attack. In certain VVER systems the characteristics of the passivating layer on steels and also concentration of soluble corrosion products seem to be in contradiction with the theoretical expectations. (author)

  4. Production and exploitation of thermoelectric air conditioning systems for vehicles

    Energy Technology Data Exchange (ETDEWEB)

    Dudnik, Vladimir [Conditioner Ltd, Gagarin (Russian Federation); Skipidarov, Sergey [SCTB NORD, Moskau (Russian Federation); Rapp, Axel [Quick-Ohm Kupper und Co. GmbH, Wuppertal-Cronenberg (Germany)

    2011-07-01

    In the paper more than 10-year experience of thermoelectric devices batch manufacturing is described for the field of their obvious advantages. This field of application includes thermoelectric air conditioning systems which have shown their competitive advantage when used in vehicles of elevated vibration where compressor equipment application is difficult because of leakage of refrigerant. Energy characteristics of air conditioners for tractors, excavators, tanks, locomotive driver's cabins and cranes are described. Thermoelectric (TE) air conditioners mechanical test data as well as operation experience in vehicles are presented. It is shown that consumption of tellurium, which is a strategic component for thermoelectric materials manufacturing, may be lowered to 40 grams per 1 kW of cooling. (orig.)

  5. Continuous fermentative hydrogen production in different process conditions

    Energy Technology Data Exchange (ETDEWEB)

    Nasirian, N. [Islamic Azad Univ., Shoushtar (Iran, Islamic Republic of). Dept. of Agricultural Mechanization; Almassi, M.; Minaee, S. [Islamic Azad Univ., Tehran (Iran, Islamic Republic of). Dept. of Agricultural Mechanization; Widmann, R. [Duisburg-Essen Univ., Essen (Germany). Dept. of Environmental Engineering, Waste and Water

    2010-07-01

    This paper reported on a study in which hydrogen was produced by fermentation of biomass. A continuous process using a non-sterile substrate with a readily available mixed microflora was used on heat treated digested sewage sludge from a wastewater treatment plant. Hydrogen was produced from waste sugar at a pH of 5.2 and a temperature of 37 degrees C. An experimental setup of three 5.5 L working volume continuously stirred tank reactors (CSTR) in different stirring speeds were constructed and operated at 7 different hydraulic retention times (HRTs) and different organic loading rates (OLR). Dissolved organic carbon was examined. The results showed that the stirring speed of 135 rpm had a beneficial effect on hydrogen fermentation. The best performance was obtained in 135 rpm and 8 h of HRT. The amount of gas varied with different OLRs, but could be stabilized on a high level. Methane was not detected when the HRT was less than 16 h. The study identified the reactor in which the highest specific rate of hydrogen production occurred.

  6. A strategy for clone selection under different production conditions.

    Science.gov (United States)

    Legmann, Rachel; Benoit, Brian; Fedechko, Ronald W; Deppeler, Cynthia L; Srinivasan, Sriram; Robins, Russell H; McCormick, Ellen L; Ferrick, David A; Rodgers, Seth T; Russo, A Peter

    2011-01-01

    Top performing clones have failed at the manufacturing scale while the true best performer may have been rejected early in the screening process. Therefore, the ability to screen multiple clones in complex fed-batch processes using multiple process variations can be used to assess robustness and to identify critical factors. This dynamic ranking of clones' strategy requires the execution of many parallel experiments than traditional approaches. Therefore, this approach is best suited for micro-bioreactor models which can perform hundreds of experiments quickly and efficiently. In this study, a fully monitored and controlled small scale platform was used to screen eight CHO clones producing a recombinant monoclonal antibody across several process variations, including different feeding strategies, temperature shifts and pH control profiles. The first screen utilized 240 micro-bioreactors were run for two weeks for this assessment of the scale-down model as a high-throughput tool for clone evaluation. The richness of the outcome data enable to clearly identify the best and worst clone as well as process in term of maximum monoclonal antibody titer. The follow-up comparison study utilized 180 micro-bioreactors in a full factorial design and a subset of 12 clone/process combinations was selected to be run parallel in duplicate shake flasks. Good correlation between the micro-bioreactor predictions and those made in shake flasks with a Pearson correlation value of 0.94. The results also demonstrate that this micro-scale system can perform clone screening and process optimization for gaining significant titer improvements simultaneously. This dynamic ranking strategy can support better choices of production clones. Copyright © 2011 American Institute of Chemical Engineers (AIChE).

  7. Importance and condition of forage crops seed production in agriculture of the Republic of Serbia

    OpenAIRE

    Đokić Dragoslav; Terzić Dragan; Milenković Jasmina; Dinić Bora; Anđelković Bojan; Stanisavljević Rade; Barać Saša

    2013-01-01

    For contemporary and economical livestock production, especially cattle and sheep raising, it is necessary to achieve high production of livestock feed while reducing production costs. Improving the production of perennial grasses and legumes creates a good basis for the development of livestock production in different agro-ecological conditions of Serbia. It also establishes a link between farming and animal husbandry, which is of particular importance for the preservation and higher fertili...

  8. Assessing the Relationship Between Chronic Health Conditions and Productivity Loss Trajectories

    Science.gov (United States)

    Pranksy, Glenn

    2014-01-01

    Objective: To examine the relationship between health conditions and the risk for membership in longitudinal trajectories of productivity loss. Methods: Trajectories of productivity loss from the ages of 25 to 44 years, previously identified in the National Longitudinal Survey of Youth (NLSY79), were combined with information on health conditions from the age 40 years health module in the NLSY79. Multinomial logistic regression was used to examine the relative risk of being in the low-risk, early-onset increasing risk, late-onset increasing risk, or high-risk trajectories compared with the no-risk trajectory for having various health conditions. Results: The trajectories with the greatest probability of productivity loss longitudinally had a greater prevalence of the individual health conditions and a greater total number of health conditions experienced. Conclusions: Health conditions are associated with specific longitudinal patterns of experiencing productivity loss. PMID:25479294

  9. Stability studies needed to define the handling and transport conditions of sensitive pharmaceutical or biotechnological products.

    Science.gov (United States)

    Ammann, Claude

    2011-12-01

    Many pharmaceutical or biotechnological products require transport using temperature-controlled systems to keep their therapeutic properties. There are presently no official guidelines for testing pharmaceutical products in order to define suitable transport specifications. After reviewing the current guidance documents, this paper proposes a methodology for testing pharmaceutical products and defining appropriate transport conditions.

  10. Effects of fermentation conditions on valuable products of ethanolic fungus Mucor indicus

    Directory of Open Access Journals (Sweden)

    Shabnam Sharifyazd

    2017-11-01

    Conclusions: It is not possible to have the maximum amounts of the products simultaneously. The fermentation conditions and composition of culture media determine the product yields. Carbon source type and the addition of nitrogen source are among the most influencing factors on the product yields. Moreover, all measured products were made with higher yields in cultivation on glucose, except glucosamine, which was produced with higher yields on xylose.

  11. Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions

    KAUST Repository

    Al Rowaihi, Israa Salem; Paillier, Alexis; Rasul, Shahid; Karan, Ram; Grö tzinger, Stefan Wolfgang; Takanabe, Kazuhiro; Eppinger, Jö rg

    2018-01-01

    extorquens AM1 and Cupriavidus necator H16. This setup allows to investigate the influence of different stress conditions, such as coexisting electrolysis, relatively high salinity, nutrient limitation, and starvation, on the production of PHB. The overall

  12. Theoretical framework on selected core issues on conditions for productive learning in networked learning environments

    DEFF Research Database (Denmark)

    Dirckinck-Holmfeld, Lone; Svendsen, Brian Møller; Ponti, Marisa

    The report documents and summarises the elements and dimensions that have been identified to describe and analyse the case studies collected in the Kaleidoscope Jointly Executed Integrating Research Project (JEIRP) on Conditions for productive learning in network learning environments.......The report documents and summarises the elements and dimensions that have been identified to describe and analyse the case studies collected in the Kaleidoscope Jointly Executed Integrating Research Project (JEIRP) on Conditions for productive learning in network learning environments....

  13. Operating conditions of T.B.P. line uranium purification plant, for uranium dioxide production

    International Nuclear Information System (INIS)

    Vardich, R.N.; La Gamma, A.M.; Anasco, R.; Soler, S.M.G. de; Isnardi, E.; Gea, V.; Chiaraviglio, R.; Matyjasczyk, E.; Aramayo, R.

    1992-01-01

    In this contribution are presented the operative conditions and the results obtained step of the Uranium dioxide production plant of Argentina. The refining step involve the Uranium concentrate dissolution, the silica ageing, the filtration and liquid - liquid extraction with n-tributyl phosphate solution in kerosene. The established operative conditions allow to obtain Uranyl nitrate solutions of nuclear purity in industrial scale. (author)

  14. Trends in the market of poultry production in the conditions of globalization

    Directory of Open Access Journals (Sweden)

    Kyryliuk O.F.

    2017-12-01

    Full Text Available according to the author, in modern conditions of globalization of food markets, the researching the state of the domestic market of poultry products in order to provide the population with high-grade food products is important. It is established that the formation of demand in the poultry market is mainly due to production by domestic producers, while imports for the period under review are being reduced and meet mainly the needs of processing industry enterprises.

  15. Assessment of biogas production from MBT waste under different operating conditions

    DEFF Research Database (Denmark)

    Pantini, Sara; Verginelli, Jason; Lombardi, Francesco

    2015-01-01

    In this work, the influence of different operating conditions on the biogas production from mechanically-. biologically treated (MBT) wastes is investigated. Specifically, different lab-scale anaerobic tests varying the water content (26-43% w/w up to 75% w/w), the temperature (from 20 to 25......, the obtained results highlighted that the operative conditions can drastically affect the gas production from MET wastes. This suggests that particular caution should be paid when using the results of lab-scale tests for the evaluation of long-term behaviour expected in the field where the boundary conditions...

  16. Degradation of the pharmaceuticals diclofenac and sulfamethoxazole and their transformation products under controlled environmental conditions

    International Nuclear Information System (INIS)

    Poirier-Larabie, S.; Segura, P.A.; Gagnon, C.

    2016-01-01

    Contamination of the aquatic environment by pharmaceuticals via urban effluents is well known. Several classes of drugs have been identified in waterways surrounding these effluents in the last 15 years. To better understand the fate of pharmaceuticals in ecosystems, degradation processes need to be investigated and transformation products must be identified. Thus, this study presents the first comparative study between three different natural environmental conditions: photolysis and biodegradation in aerobic and anaerobic conditions both in the dark of diclofenac and sulfamethoxazole, two common drugs present in significant amounts in impacted surface waters. Results indicated that degradation kinetics differed depending on the process and the type of drug and the observed transformation products also differed among these exposure conditions. Diclofenac was nearly degraded by photolysis after 4 days, while its concentration only decreased by 42% after 57 days of exposure to bacteria in aerobic media and barely 1% in anaerobic media. For sulfamethoxazole, 84% of the initial concentration was still present after 11 days of exposure to light, while biodegradation decreased its concentration by 33% after 58 days of exposure under aerobic conditions and 5% after 70 days of anaerobic exposure. In addition, several transformation products were observed and persisted over time while others degraded in turn. For diclofenac, chlorine atoms were lost primarily in the photolysis, while a redox reaction was promoted by biodegradation under aerobic conditions. For sulfamethoxazole, isomerization was favored by photolysis while a redox reaction was also favored by the biodegradation under aerobic conditions. To summarize this study points out the occurrence of different transformation products under variable degradation conditions and demonstrates that specific functional groups are involved in the tested natural attenuation processes. Given the complexity of environmental

  17. Degradation of the pharmaceuticals diclofenac and sulfamethoxazole and their transformation products under controlled environmental conditions

    Energy Technology Data Exchange (ETDEWEB)

    Poirier-Larabie, S. [Aquatic Contaminants Research Division, Science and Water Technology Directorate, Environment Canada, Montréal, Québec H2Y 2E7 (Canada); Segura, P.A. [Department of Chemistry, Université de Sherbrooke, Sherbrooke, Québec J1K 2R1 (Canada); Gagnon, C., E-mail: christian.gagnon@canada.ca [Aquatic Contaminants Research Division, Science and Water Technology Directorate, Environment Canada, Montréal, Québec H2Y 2E7 (Canada)

    2016-07-01

    Contamination of the aquatic environment by pharmaceuticals via urban effluents is well known. Several classes of drugs have been identified in waterways surrounding these effluents in the last 15 years. To better understand the fate of pharmaceuticals in ecosystems, degradation processes need to be investigated and transformation products must be identified. Thus, this study presents the first comparative study between three different natural environmental conditions: photolysis and biodegradation in aerobic and anaerobic conditions both in the dark of diclofenac and sulfamethoxazole, two common drugs present in significant amounts in impacted surface waters. Results indicated that degradation kinetics differed depending on the process and the type of drug and the observed transformation products also differed among these exposure conditions. Diclofenac was nearly degraded by photolysis after 4 days, while its concentration only decreased by 42% after 57 days of exposure to bacteria in aerobic media and barely 1% in anaerobic media. For sulfamethoxazole, 84% of the initial concentration was still present after 11 days of exposure to light, while biodegradation decreased its concentration by 33% after 58 days of exposure under aerobic conditions and 5% after 70 days of anaerobic exposure. In addition, several transformation products were observed and persisted over time while others degraded in turn. For diclofenac, chlorine atoms were lost primarily in the photolysis, while a redox reaction was promoted by biodegradation under aerobic conditions. For sulfamethoxazole, isomerization was favored by photolysis while a redox reaction was also favored by the biodegradation under aerobic conditions. To summarize this study points out the occurrence of different transformation products under variable degradation conditions and demonstrates that specific functional groups are involved in the tested natural attenuation processes. Given the complexity of environmental

  18. Conditions for uniqueness of product representations for separable quantum channels and separable quantum states

    International Nuclear Information System (INIS)

    Cohen, Scott M.

    2014-01-01

    We give a sufficient condition that an operator sum representation of a separable quantum channel in terms of product operators is the unique product representation for that channel, and then provide examples of such channels for any number of parties. This result has implications for efforts to determine whether or not a given separable channel can be exactly implemented by local operations and classical communication. By the Choi-Jamiolkowski isomorphism, it also translates to a condition for the uniqueness of product state ensembles representing a given quantum state. These ideas follow from considerations concerning whether or not a subspace spanned by a given set of product operators contains at least one additional product operator

  19. Production conditions of bioenergy in Swedish agriculture; Produktionsfoerutsaettningar foer biobraenslen inom svenskt jordbruk

    Energy Technology Data Exchange (ETDEWEB)

    Boerjesson, Paal

    2007-05-15

    The overall aim of this report is to analyse and describe the production conditions of bioenergy in Swedish agriculture and how these conditions can vary due to different factors. The conclusion is that the potential for producing bioenergy in Swedish agriculture will vary significantly depending on which energy crops are cultivated, which type of agricultural land is utilised and the geographical location of the production. Furthermore, different crop residues and other by-products from agriculture, utilised for energy purposes, will affect the bioenergy potential. To which extent this physical/biological potential will be utilised in the future depends mainly on economic conditions and financial considerations. These aspects are not included in this study. The report starts with a description of current crop production in Sweden, expressed in energy terms, the energy needed for this production and the regional variation in crop yields. The local variations in cultivation conditions are also analysed, as well as variations over the area of a single farm. Another aspect discussed is the production conditions of energy crops on previous farm land not currently utilised. The report includes an analysis of the potential supply of crop residues and other by-products for energy purposes, such as straw, tops and leaves of sugar beets, manure etc, as well as the regional variation of these residues and by-products. A similar analysis is made of the regional production conditions and potential biomass yields of traditional crops and new energy crops. These analyses also include energy balance calculations showing the energy input needed for different production systems in relation to the harvested biomass yield, and the potential for increased biomass yields in the future. Based on the findings of these various analyses, calculations are made showing some examples of how much bioenergy Swedish agriculture can deliver, depending on how much agricultural land is utilised for

  20. Influence of high gravity process conditions on the environmental impact of ethanol production from wheat straw

    DEFF Research Database (Denmark)

    Janssen, Matty; Tillman, Anne-Marie; Cannella, David

    2014-01-01

    Biofuel production processes at high gravity are currently under development. Most of these processes however use sugars or first generation feedstocks as substrate. This paper presents the results of a life cycle assessment (LCA) of the production of bio-ethanol at high gravity conditions from...... of the ethanol production, but this can be compensated by reducing the impact of enzyme production and use, and by polyethylene glycol addition at high dry matter content. The results also show that the renewable and non-renewable energy use resulting from the different process configurations ultimately...

  1. Influence of product thickness, chamber pressure and heating conditions on production rate of freeze-dried yoghurt

    Energy Technology Data Exchange (ETDEWEB)

    Sharma, N.K. [G.B. Pant Univ., of Agriculture and Technology (India). Dept. of Mechanical Engineering; Arora, C.P. [Indian Inst. of Tech., New Delhi (India)

    1995-06-01

    The effects of product thickness, chamber pressure and heating conditions on product temperature profiles and production rate of freeze-dried yoghurt were investigated experimentally. Three sample thicknesses - 3.8 mm, 6.2 mm and 9.4 mm - were tested at chamber pressures of 0.01 and 0.5 mmHg. The production rate increased by decreasing product thickness in contact heating through the bottom of the frozen layer, whereas no significant change was observed in radiant heating. A reduction in chamber pressure from 0.50 to 0.01 mmHg increased the drying time in radiant heating. Maximum production rate was obtained when the thickness of dried product was 6.2 mm, when heat was transferred simultaneously through the frozen and dried layers, and the chamber pressure was at 0.01 mmHg. Use of the product tray developed in this study prevents the growth of dry layers at the contact surfaces. (Author)

  2. Optimization of cultural conditions for biosurfactant production by Pleurotus djamor in solid state fermentation.

    Science.gov (United States)

    Velioglu, Zulfiye; Ozturk Urek, Raziye

    2015-11-01

    Being eco-friendly, less toxic, more biodegradable and biocompatible, biological surfactants have higher activity and stability compared to synthetic ones. In spite of the fact that there are abundant benefits of biosurfactants over the synthetic congeners, the problem related with the economical and large scale production proceeds. The utilization of several industrial wastes in the production media as substrates reduces the production cost. This current study aims optimization of biosurfactant production conditions by Pleurotus djamor, grown on sunflower seed shell, grape wastes or potato peels as renewable cheap substrates in solid state fermentation. After determination of the best substrate for biosurfactant production, we indicate optimum size and amount of solid substrate, volume of medium, temperature, pH and Fe(2+) concentrations on biosurfactant production. In optimum conditions, by reducing water surface tension to 28.82 ± 0.3 mN/m and having oil displacement diameter of 3.9 ± 0.3 cm, 10.205 ± 0.5 g/l biosurfactant was produced. Moreover, chemical composition of biosurfactant produced in optimum condition was determined by FTIR. Lastly, laboratory's large-scale production was carried out in optimum conditions in a tray bioreactor designed by us and 8.9 ± 0.5 g/l biosurfactant was produced with a significant surface activity (37.74 ± 0.3 mN/m). With its economical suggestions and applicability of laboratory's large-scale production, this work indicates the possibility of using low cost agro-industrial wastes as renewable substrates for biosurfactant production. Therefore, using economically produced biosurfactant will reduce cost in several applications such as bioremediation, oil recovery and biodegradation of toxic chemicals. Copyright © 2015 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  3. Calorimetric study on human erythrocyte glycolysis. Heat production in various metabolic conditions.

    Science.gov (United States)

    Minakami, S; de Verdier, C H

    1976-06-01

    The heat production of human erythrocytes was measured on a flow microcalorimeter with simultaneous analyses of lactate and other metabolites. The heat production connected with the lactate formation was about 17 kcal (71 kJ) per mol lactate formed which corresponded to the sum of heat production due to the formation of lactate from glucose and the heat production due to neutralization. The heat production rate increased as the pH of the suspension increased, corresponding to the increase in lactate formation. Glycolytic inhibitors such as fluoride and monoiodoacetate caused a decrease in the rate of heat production, whereas arsenate induced a large transient increase in heat production associated with a transient increase in lactate formation. Decrease in pyruvate concentration was usually associated with increase in heat production, although the decreased pyruvate concentration was coupled with formation of 2,3-bisphosphoglycerate. When inosine, dihydroxyacetone or D-glyceraldehyde was used as a substrate, an increase in the heat production rate was observed. Addition of methylene blue caused an oxygen uptake which was accompanied by a remarkable increase in heat production rate corresponding to about 160 kcal (670 kJ) per mol oxygen consumed. The value for heat production in red cells in the above-mentioned metabolic conditions was considered in relation to earlier known data on free energy and enthalpy changes of the different metabolic steps in the glycolytic pathway.

  4. Importance and condition of forage crops seed production in agriculture of the Republic of Serbia

    Directory of Open Access Journals (Sweden)

    Đokić Dragoslav

    2013-01-01

    Full Text Available For contemporary and economical livestock production, especially cattle and sheep raising, it is necessary to achieve high production of livestock feed while reducing production costs. Improving the production of perennial grasses and legumes creates a good basis for the development of livestock production in different agro-ecological conditions of Serbia. It also establishes a link between farming and animal husbandry, which is of particular importance for the preservation and higher fertility of arable land and the protection of agro-ecosystems. An important factor for the cheaper production of livestock feed is the possibility to provide sufficient quantities of quality seeds at affordable prices. Production of quality seeds of local varieties of perennial legumes is possible to obtain sufficient amounts of good quality forage. Current situation in forage crop seed production of the Republic of Serbia is unsatisfactory because the seed of perennial grasses are mostly imported. Domestic production of alfalfa, red clover and birdsfoot trefoil met domestic needs only in some years. Seed of imported varieties are often not satisfactory because those varieties are not adapted to our local agro-ecological conditions. The present results provide the basis and direction for further researches that may provide solutions to increase seed yields and which will be widely accepted in practice, which will make the production more cost-effective. Institute for forage crops Kruševac is making a significant contribution to the development of technology of seed productions, especially alfalfa, red clover and perennial grasses. Therefore the role of the Institute is very important and necessary link between production, processing and trading seeds of perennial legumes and grasses in Serbia.

  5. Soil aptitude for the production of sugarcane. Part I. Calibration in experimental and production conditions

    Directory of Open Access Journals (Sweden)

    Nelson C. Arzola Pina

    2015-02-01

    Full Text Available A database was created using the yield values and the soil characteristics from more than 50 field experiments, and from other areas that belonged to good producers in Cienfuegos. All the experiments were done on brown carbonated, brown non-carbonated and red ferralitic soils. The soils characteristics were selected based on a varied analysis of main components and taking the easily-determined variables. For each soil characteristic that was selected, a classification system was established, that encompasses several variable ranges, the category assigned to each range, and a point value for each category. This classification system was based on articles reviews, and also taking into account the results of many researchers. A linear regression analysis was done using sugarcane yield and the values of soils where the sugar cane was planted and the cases of “excellent management” and “adequate management” of the cultivar was discriminated. It was proved that the soil value and the sugarcane yield were highly related (linear regression, which made possible the elaboration of a table that relates the sugarcane productive potential according to the characteristics of the soil expressed by the accumulated points, depending on the quality of the cultivar management and the agricultural cycle (plant cane or ratoon.

  6. Production bias: A proposed modification of the driving force for void swelling under cascade damage conditions

    International Nuclear Information System (INIS)

    Woo, C.H.; Garner, F.A.

    1991-11-01

    A new concept of point-defect production as the main driving force for void swelling under cascade damage conditions is proposed. This concept takes into account the recombination and formation of immobile clusters and loops of vacancies and interstitials in the cascade region. The life times of the clusters and loops due to desolution are strong functions of the temperature, as well as their vacancy and interstitial nature. The resulting biased production of free point defects from the internal sources is shown to be a strong driving force for void swelling. The characteristics of void swelling due to production bias are described and compared with experimental results. We conclude that the production bias concept provides a good description of void swelling under cascade damage conditions

  7. Production bias: A proposed modification of the driving force for void swelling under cascade damage conditions

    International Nuclear Information System (INIS)

    Woo, C.H.; Singh, B.N.; Garner, F.A.

    1992-01-01

    A new concept of point defect production as the main driving force for void swelling under cascade damage conditions is proposed. This concept takes into account the recombination and formation of immobile clusters and loops of vacancies and interstitials in the cascade region. The lifetimes of the clusters and loops due to desolution are strong functions of the temperature, as well as their vacancy and interstitial nature. The resulting biased production of free point defects from the internal sources is shown to be a strong driving force for void swelling. The characteristics of void swelling due to production bias are described and compared with experimental results. We conclude that the production bias concept provides a good description of void swelling under cascade damage conditions. (orig.)

  8. Biodiesel production process optimization and characterization to assess the suitability of the product for varied environmental conditions

    Energy Technology Data Exchange (ETDEWEB)

    Eevera, T.; Rajendran, K.; Saradha, S. [Department of Biotechnology, Periyar Maniammai University, Periyar Nagar, Vallam, Thanjavur, Tamilnadu 613 403 (India)

    2009-03-15

    In this study, both edible (coconut oil, palm oil, groundnut oil, and rice bran oil) and non-edible oils (pongamia, neem and cotton seed oil) were used to optimize the biodiesel production process variables like catalyst concentration, amount of methanol required for reaction, reaction time and reaction temperature. The fuel properties like specific gravity, moisture content, refractive index, acid value, iodine number, saponification value and peroxide value were estimated. Based on the cetane number and iodine value, the methyl esters obtained from palm and coconut oils were not suitable to use as biodiesel in cold weather conditions, but for hot climate condition biodiesel obtained from the remaining oil sources is suitable. (author)

  9. Diffusion modeling of fission product release during depressurized core conduction cooldown conditions

    International Nuclear Information System (INIS)

    Martin, R.C.

    1991-01-01

    A simple model for diffusion through the silicon carbide layer of TRISO particles is applied to the data for accident condition testing of fuel spheres for the High-Temperature Reactor program of the Federal Republic of Germany (FRG). Categorization of sphere release of 137 Cs based on fast neutron fluence permits predictions of release with an accuracy comparable to that of the US/FRG accident condition fuel performance model. Calculations are also performed for 85 Kr, 90 Sr, and 110m Ag. Diffusion of cesium through SiC suggests that models of fuel failure should consider fuel performance during repeated accident condition thermal cycling. Microstructural considerations in models of fission product release are discussed. The neutron-induced segregation of silicon within the SiC structure is postulated as a mechanism for enhanced fission product release during accident conditions. As oxygen-enhanced SiC decomposition mechanism is also discussed. (author). 12 refs, 11 figs, 2 tabs

  10. Comparing Effects of Feedstock and Run Conditions on Pyrolysis Products Produced at Pilot-Scale

    Energy Technology Data Exchange (ETDEWEB)

    Dunning, Timothy C [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Gaston, Katherine R [National Renewable Energy Laboratory (NREL), Golden, CO (United States); Wilcox, Esther [National Renewable Energy Laboratory (NREL), Golden, CO (United States)

    2018-01-19

    Fast pyrolysis is a promising pathway for mass production of liquid transportable biofuels. The Thermochemical Process Development Unit (TCPDU) pilot plant at NREL is conducting research to support the Bioenergy Technologies Office's 2017 goal of a $3 per gallon biofuel. In preparation for down select of feedstock and run conditions, four different feedstocks were run at three different run conditions. The products produced were characterized extensively. Hot pyrolysis vapors and light gasses were analyzed on a slip stream, and oil and char samples were characterized post run.

  11. Local power production at the end consumer - legal, political and economical external conditions

    International Nuclear Information System (INIS)

    Grinden, Bjoern; Hunnes, Arngrim; Naesje, Paal; Wangensteen, Ivar; Morch, Andrei Z.

    2002-12-01

    The report deals with the external conditions for local power production, suggested as a production close to or at the end consumer. The political, legal and economical frame conditions for such production including rating are discussed. The report shall together with a technical report regarding appropriate technologies for such production (A5712), serve as a basis for case studies and monitors later in the project. Through the case studies it will be uncovered how the external conditions are functioning which will make foundations for recommendations concerning possible alterations in the conditions in order to make the local power production more profitable. In the discussion on the political and legal external conditions the system of today is studied. From the political area the general development is described and a short analysis is made of what to expect from case handling procedures, and some challenges are pointed out At present there is a simplified handling of cases of minor and smaller power plants. In order to obtain a more realistic construction of such plants the requirements of license handling may need sharpening. The tariffing of energy deliverance is studied. The regulations for tariffing and income regulation in the distribution network is mainly designed with the consumer and the central power production in mind. A study is made of how the regulations work, to what extent precessions and additional rules are needed and to what extent alterations in the regulations are needed in order to incorporate the local power production in a rational way. While a local power producer at best, will want a price for power which is sold at the power market of the size of 20 oere/kWh, the power will increase in value further down in the voltage level. At the 230 V level the power price will be of the size of 60 oere/kWh all expenses included and the network rent (during normal precipitation conditions). Therefore the production for own consumption will be met

  12. Green Fodder Production and Water Use Efficiency of Some Forage Crops under Hydroponic Conditions

    OpenAIRE

    Ghazi N. Al-Karaki; M. Al-Hashimi

    2012-01-01

    The objectives of this study were to evaluate five forage crops (alfalfa (Medicago sativa), barley (Hordeum vulgare), cowpea (Vigna unguiculata), sorghum (Sorghum bicolor), and wheat (Triticum aestivum)) for green fodder production and water use efficiency under hydroponic conditions. The experiment has been conducted under temperature-controlled conditions (24 ± 1°C) and natural window illumination at growth room of Soilless Culture Laboratory, Arabian Gulf University, Manama, Bahrain. The r...

  13. USDA Foreign Agricultural Service overview for operational monitoring of current crop conditions and production forecasts.

    Science.gov (United States)

    Crutchfield, J.

    2016-12-01

    The presentation will discuss the current status of the International Production Assessment Division of the USDA ForeignAgricultural Service for operational monitoring and forecasting of current crop conditions, and anticipated productionchanges to produce monthly, multi-source consensus reports on global crop conditions including the use of Earthobservations (EO) from satellite and in situ sources.United States Department of Agriculture (USDA) Foreign Agricultural Service (FAS) International Production AssessmentDivision (IPAD) deals exclusively with global crop production forecasting and agricultural analysis in support of the USDAWorld Agricultural Outlook Board (WAOB) lockup process and contributions to the World Agricultural Supply DemandEstimates (WASE) report. Analysts are responsible for discrete regions or countries and conduct in-depth long-termresearch into national agricultural statistics, farming systems, climatic, environmental, and economic factors affectingcrop production. IPAD analysts become highly valued cross-commodity specialists over time, and are routinely soughtout for specialized analyses to support governmental studies. IPAD is responsible for grain, oilseed, and cotton analysison a global basis. IPAD is unique in the tools it uses to analyze crop conditions around the world, including customweather analysis software and databases, satellite imagery and value-added image interpretation products. It alsoincorporates all traditional agricultural intelligence resources into its forecasting program, to make the fullest use ofavailable information in its operational commodity forecasts and analysis. International travel and training play animportant role in learning about foreign agricultural production systems and in developing analyst knowledge andcapabilities.

  14. Robust Production Planning in Fashion Apparel Industry under Demand Uncertainty via Conditional Value at Risk

    Directory of Open Access Journals (Sweden)

    Abderrahim Ait-Alla

    2014-01-01

    Full Text Available This paper presents a mathematical model for robust production planning. The model helps fashion apparel suppliers in making decisions concerning allocation of production orders to different production plants characterized by different lead times and production costs, and in proper time scheduling and sequencing of these production orders. The model aims at optimizing these decisions concerning objectives of minimal production costs and minimal tardiness. It considers several factors such as the stochastic nature of customer demand, differences in production and transport costs and transport times between production plants in different regions. Finally, the model is applied to a case study. The results of numerical computations are presented. The implications of the model results on different fashion related product types and delivery strategies, as well as the model’s limitations and potentials for expansion, are discussed. Results indicate that the production planning model using conditional value at risk (CVaR as the risk measure performs robustly and provides flexibility in decision analysis between different scenarios.

  15. Characterisation of non-aerosol-bound fractions of radon decay products under environmental conditions

    International Nuclear Information System (INIS)

    Pagelkopf, P.; Porstendoerfer, J.

    2004-01-01

    Dose-relevant factors such as the concentration and size distribution of radon decay products are strongly influenced by the charge-carrying fraction and state of charge of the first radon decay product, 2 18Po. The charge of 2 18Po influences its own mobility and hence its attachment to aerosols and deposition on surfaces, also referred to as ''plating out''. The mobility of 2 18Po can be described in terms of its diffusion coefficient. The goal of the present study was to determine theoretically as well as practically the charge-carrying fraction of radon decay products 2 18Po and 2 14Pb under room air conditions and to design and construct an electrical mobility spectrometer. The spatial model developed by Jacobi and modified by Porstendoerfer for calculating the concentration of unattached radon decay products in indoor and outdoor air under steady-state conditions was extended to permit a differentiated description of the charge-carrying and neutral unattached fractions of radon decay products 2 18Po and 2 14Pb. An 8 m 2 chamber permitting chamber air control in terms of radon gas concentration and humidity was built in order to study the behaviour of radon decay products. The charge-carrying fraction of unattached radon decay products 218 Po and 214 Pb was measured in this chamber. A technique referred to as the online backscreen technique (OBST) was developed to permit the continuous measurement of unattached decay products. The technique involves the diffusive deposition of unattached radon decay products from a laminar flow onto a wire lattice and their subsequent measurement. The total fraction of decay products is then determined by means of the filtration method. Furthermore, all parameters required for modelling such as radon gas, decay product and aerosol concentrations as well as air humidity, temperature and ion dose rate were measured [de

  16. LIMNOLOGICAL CONDITION AND ESTIMATION OF POTENTIAL FISH PRODUCTION OF KERINCI LAKE JAMBI, SUMATRA

    Directory of Open Access Journals (Sweden)

    Samuel Samuel

    2015-06-01

    Full Text Available Kerinci Lake is a type of tectonic lakes located in a protected forest area of National Park of Kerinci Sebelat and a source of various fish species important for local people for their dayly food comsumption and income. However, few information is available on limnological condition and fish resources. Field research observing the limnological condition and estimating the potential fish production was conducted four times in April, June, August and October 2013. The research is aimed to describe the condition of limnology and estimate the potential fish production of the lake. Limnological aspect included the physico-chemical and biological parameters, namely: temperature, water transparency, depth, substrate, conductivity, pH, dissolved oxygen, alkalinity, ammonia, nitrate, phosphate, total phosphorus, chlorophyll-a and trophic state. Potential fish production was calculated by using the biological parameter levels of chlorophyll-a. The results show that the euphotic layer of the lake waters was still feasible for fish life. Water condition of the bottom layer was less supportable for fish life due to low dissolved oxygen content. Trophic state index (TSI values, either measured by temporal and spatial ways, had TSI with an average of 61.75. From these index, the lake is classified as a lake at the high productivity level (eutrophic. Annual fish production was an average of 307 kg/ha/year. By taking account the average of fish production and the total area of lake of around 4,200 ha, the potential fish production of Kerinci Lake is estimated about ± 1,287 tons/year.

  17. Enhancing Ethanol Production by Fermentation Using Saccharomyces cereviseae under Vacuum Condition in Batch Operation

    Directory of Open Access Journals (Sweden)

    A Abdullah

    2012-02-01

    Full Text Available Ethanol is one of renewable energy, which considered being an excellent alternative clean-burning fuel to replaced gasoline. In fact, the application of ethanol as fuel still blended with gasoline. The advantages of using ethanol as fuel are that the raw material mostly from renewable resources and the product has low emission which means environmental friendly. Ethanol can be produced by fermentation of sugars (glucose/fructose. The constraint in the ethanol fermentation batch or continuous process is the ethanol product inhibition. Inhibition in ethanol productivity and cell growth can be overcome by taking the product continuously from the fermentor. The process can be done by using a vacuum fermentation. The objective of this research is to investigate the effect of pressure and glucose concentration in ethanol fermentation. The research was conducted in laboratory scale and batch process. Equipment consists of fermentor with vacuum system. The observed responses were dried cells of yeast, concentration of glucose, and concentration of ethanol. Observations were made every 4 hours during a day of experiment. The results show that the formation of ethanol has a growth-associated product characteristic under vacuum operation. Vacuum condition can increase the cell formation productivity and the ethanol formation, as it is compared with fermentation under atmospheric condition. The maximum cells productivity and ethanol formation in batch operation under vacuum condition was reached at 166.6 mmHg of pressure. The maximum numbers of cells and ethanol formation was reached at 141.2 mm Hg of pressure. High initial glucose concentration significantly can affect the productivity and the yield of ethanol.

  18. Compatibility of Encore's formosa (Hymenoptera: aphelinidae) with commercial products under laboratory conditions

    International Nuclear Information System (INIS)

    Barrera Mojica, Jennifer; Carrascal, Jesus Armando; Numa, Stephanie; Rodriguez, Daniel; Cantor, Fernando

    2013-01-01

    The greenhouse white fly, Trialeurodes vaporariorum one of the major pests of tomatoes under greenhouse conditions, but, it's mainly controlled with chemical insecticides. However, there are alternative control strategies as entomopathogens fungi and parasitoids (Encarsia formosa). In this study we evaluated the compatibility of commercial product with adults and pupae of E. formosa under laboratory conditions. eight treatments were evaluated for adults and pupae of the parasitoid, including two concentrations of the entomopathogenic fungus Beauveria bassiana (5 x 107 and 5 x 109 conidia/ml), commercial doses of four chemical products commonly used to control pests on tomato crops and two controls. These treatments were applied directly on parasitoids, and their mortality rate on adults and pupae were recorded daily. There was no difference in E. formosa pupae among relative with the bacillus thuringiensis products (p > 0.05), indicating that it's compatible with the parasitoid pupae under laboratory conditions. Finally, it was found that products with active ingredients of tiocyclam hidrogenoxalato and B. thuringiensis are incompatibles with E. formosa adults under laboratory conditions.

  19. Biomass and cellulosic ethanol production of forage sorghum under limited water conditions

    Science.gov (United States)

    A two year field study was conducted to evaluate biofuel production potential of two forage sorghum cultivars differing in brown midrib trait under non-irrigated and deficit irrigation conditions in the semiarid Southern High Plains of the U.S. Cultivar SP1990 (non-bmr = conventional cell wall comp...

  20. Techniques of treatment or conditioning for waste arising from 131I production

    International Nuclear Information System (INIS)

    Dellamono, J.C.

    1989-07-01

    Distillation, evaporation/crystallization, direct immobilization and some chemical like precipitation and reduction were studied as techniques of treatment or conditioning for waste arising from 131 I production. The description of all techniques studied, as well as evaluation and discussion of the results are presented. (author) [pt

  1. HYDROLOGIC CONDITIONS AFFECTING THE TROPOSPHERIC FLUX OF VINCLOZOLIN AND ITS DEGRADATION PRODUCTS

    Science.gov (United States)

    A laboratory chamber was used to determine hydrologic conditions that lead to the tropospheric flux of a suspected anti-androgenic dicarboximide fungicide, vinclozolin (3-(3,5-dichlorophenyl)-5-methyl-5-vinyl-oxzoli-dine-2,4-dione) and three degradation products from sterilized...

  2. Multiscale Modeling of Poly(lactic acid) Production: From Reaction Conditions to Rheology of Polymer Melt

    DEFF Research Database (Denmark)

    Zubov, Alexandr; Sin, Gürkan

    2018-01-01

    Abstract Poly(L-lactic acid) (PLLA) is a fully biodegradable bioplastic with promising market potential. The paper deals with systematic development and analysis of the modeling framework allowing direct mapping between PLLA production process conditions and rheological properties of the polymer ...

  3. The biotechnology of hydrogen production by Nostoc flagelliforme grown under chemostat conditions

    Energy Technology Data Exchange (ETDEWEB)

    Lichtl, R.R.; Bazin, M.J.; Hall, D.O. [Div. of Life Sciences, King`s College, London Univ. (United Kingdom)

    1997-11-01

    The potential of using N{sub 2}-fixing cyanobacteria to produce hydrogen photobiologically has stimulated research on the physiology and biotechnology of species exhibiting high H{sub 2} production rates over long periods of time. In this work Nostoc flagelliforme, a terrestrial N{sub 2}-fixing cyanobacterium, has been examined to establish its physiology and potential for H{sub 2} production under controlled conditions. Cell filaments of N. flagelliforme were purified and grown in liquid culture to optimize its H{sub 2} metabolism. In batch-grown cultures the activity of nitrogenase, the key enzyme for H{sub 2} production in N{sub 2}-fixing organisms, was found to be high only during a short phase of exponential growth. A chemostat system was thus constructed for long-term experiments using continuous cultures, with the aim of exploiting the exponential growth phase. The dilution rate (D) and environmental factors, such as N{sub 2} concentration in the gas phase and temperature, significantly influenced H{sub 2} production. Cells grown continuously under the optimized conditions of D=0.022 h{sup -1}, 34 C and 5.1 kPa N{sub 2} in the gas phase exhibited H{sub 2} production rates that were more than four times higher than the maximal rates under standard batch growth conditions. (orig.)

  4. Enhancing Ethanol Production by Fermentation Using Saccharomyces cereviseae under Vacuum Condition in Batch Operation

    Directory of Open Access Journals (Sweden)

    A Abdullah

    2012-04-01

    Full Text Available Ethanol is one of renewable energy, which considered being an excellent alternativeclean-burning fuel to replaced gasoline. In fact, the application of ethanol as fuel still blended withgasoline. The advantages of using ethanol as fuel are that the raw material mostly from renewableresources and the product has low emission which means environmental friendly. Ethanol can beproduced by fermentation of sugars (glucose/fructose. The constraint in the ethanol fermentationbatch or continuous process is the ethanol product inhibition. Inhibition in ethanol productivityand cell growth can be overcome by taking the product continuously from the fermentor. Theprocess can be done by using a vacuum fermentation. The objective of this research is toinvestigate the effect of pressure and glucose concentration in ethanol fermentation. The researchwas conducted in laboratory scale and batch process. Equipment consists of fermentor withvacuum system. The observed responses were dried cells of yeast, concentration of glucose, andconcentration of ethanol. Observations were made every 4 hours during a day of experiment. Theresults show that the formation of ethanol has a growth-associated product characteristic undervacuum operation. Vacuum condition can increase the cell formation productivity and the ethanolformation, as it is compared with fermentation under atmospheric condition. The maximum cellsproductivity and ethanol formation in batch operation under vacuum condition was reached at166.6 mmHg of pressure. The maximum numbers of cells and ethanol formation was reached at141.2 mm Hg of pressure. High initial glucose concentration significantly can affect the productivityand the yield of ethanol.

  5. Heating, Ventilation, and Air Conditioning Design Strategy for a Hot-Humid Production Builder

    Energy Technology Data Exchange (ETDEWEB)

    Kerrigan, P. [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2014-03-01

    BSC worked directly with the David Weekley Homes - Houston division to redesign three floor plans in order to locate the HVAC system in conditioned space. The purpose of this project is to develop a cost effective design for moving the HVAC system into conditioned space. In addition, BSC conducted energy analysis to calculate the most economical strategy for increasing the energy performance of future production houses. This is in preparation for the upcoming code changes in 2015. The builder wishes to develop an upgrade package that will allow for a seamless transition to the new code mandate. The following research questions were addressed by this research project: 1. What is the most cost effective, best performing and most easily replicable method of locating ducts inside conditioned space for a hot-humid production home builder that constructs one and two story single family detached residences? 2. What is a cost effective and practical method of achieving 50% source energy savings vs. the 2006 International Energy Conservation Code for a hot-humid production builder? 3. How accurate are the pre-construction whole house cost estimates compared to confirmed post construction actual cost? BSC and the builder developed a duct design strategy that employs a system of dropped ceilings and attic coffers for moving the ductwork from the vented attic to conditioned space. The furnace has been moved to either a mechanical closet in the conditioned living space or a coffered space in the attic.

  6. Heating, Ventilation, and Air Conditioning Design Strategy for a Hot-Humid Production Builder

    Energy Technology Data Exchange (ETDEWEB)

    Kerrigan, P. [Building Science Corporation, Somerville, MA (United States)

    2014-03-01

    Building Science Corporation (BSC) worked directly with the David Weekley Homes - Houston division to develop a cost-effective design for moving the HVAC system into conditioned space. In addition, BSC conducted energy analysis to calculate the most economical strategy for increasing the energy performance of future production houses in preparation for the upcoming code changes in 2015. This research project addressed the following questions: 1. What is the most cost effective, best performing and most easily replicable method of locating ducts inside conditioned space for a hot-humid production home builder that constructs one and two story single family detached residences? 2. What is a cost effective and practical method of achieving 50% source energy savings vs. the 2006 International Energy Conservation Code for a hot-humid production builder? 3. How accurate are the pre-construction whole house cost estimates compared to confirmed post construction actual cost?

  7. Relationship between fumonisin production and FUM gene expression in Fusarium verticillioides under different environmental conditions.

    Science.gov (United States)

    Fanelli, Francesca; Iversen, Anita; Logrieco, Antonio F; Mulè, Giuseppina

    2013-01-01

    Fusarium verticillioides is the main source of fumonisins, a group of mycotoxins that can contaminate maize-based food and feed and cause diseases in humans and animals. The study of the effect of different environmental conditions on toxin production should provide information that can be used to develop strategies to minimize the risk. This study analysed the effect of temperature (15°C-35°C), water activity (a(w): 0.999-0.93), salinity (0-125 g l(-1) NaCl) and pH (5-8) on the growth and production of fumonisins B(1) (FB1), B(2) (FB2) and B(3) (FB3) and the expression of FUM1 and FUM21 in F. verticillioides. The highest growth rate was measured at 25°C, a(w) of 0.998-0.99 and 0-25 g l(-1) of NaCl. Optimal conditions for fumonisin production were 30°C, a(w) of 0.99, 25 g l(-1) of NaCl and pH 5; nevertheless, the strain showed a good adaptability and was able to produce moderate levels of fumonisins under a wide range of conditions. Gene expression mirrored fumonisin production profile under all conditions with the exception of temperature: FUM1 and FUM21 expression was highest at 15°C, while maximum fumonisin production was at 30°C. These data indicate that a post-transcriptional regulation mechanism could account for the different optimal temperatures for FUM gene expression and fumonisin production.

  8. Investigation of oil production conditions and production operation by solution gas drive in low permeable heterogeneous limestones

    Energy Technology Data Exchange (ETDEWEB)

    Lillie, W

    1966-04-01

    It was the purpose of this study to investigate the production of oil and gas from a low permeable heterogeneous limestone-reservoir by solution gas drive. The rock-samples were subjected to extensive petrolphysical analyses in order to characterize the pore structure of of the limestone material. Laboratory model flow tests were undertaken to outline in detail the production history during the pressure depletion process under reservoir conditions and by using original reservoir fluids. The experiments were carried out at different rates of pressure decline. It can be stated that the rate of pressure decline is the most important factor affecting the oil recovery and the development of the gas-oil-ratio in a model flow test. The present investigation leads to the following conclusion: It is posible to get reliable results which could be the base for a reservoir performance prediction only when the gas and oil phase are maintained at equilibrium conditions within the rock sample during the pressure decline. An additional calculation of the solution gas drive reservoir production history by the Tarner method shows a good agreement of the experimental and the calculated data. (40 refs.)

  9. Recruitment and condition of juvenile sandeel on the Faroe shelf in relation to primary production

    DEFF Research Database (Denmark)

    Eliasen, Kirstin; Reinert, Jákup; Gaard, Eilif

    The food of early-life sandeel is dominated by zooplankton, which again depends on primary production. On the Faroe Shelf, measurements of accumulated new primary production and chlorophyll a during spring and summer have been carried out since 1990 and 1997, respectively. Large inter...... availability. We compare the time series from the sandeel 0-group surveys with data on phytoplankton production and biomass. The results confirm that survival and condition of the early-life stages of sandeel on the Faroe Shelf is dependent on the magnitude of the primary production. Although the sandeel......-annual variations in the onset of the spring bloom and its intensity have been observed. Since 1974 juvenile sandeels have been sampled annually on the Faroe shelf. These results also show large variations – both in number and in average length. Here, we investigate the variations in recruitment in relation to food...

  10. Production of functional killer protein in batch cultures upon a shift from aerobic to anaerobic conditions

    Directory of Open Access Journals (Sweden)

    Gildo Almeida da Silva

    2011-06-01

    Full Text Available The aim of this work was to study the production of functional protein in yeast culture. The cells of Saccharomyces cerevisiae Embrapa 1B (K+R+ killed a strain of Saccharomyces cerevisiae Embrapa 26B (K-R-in grape must and YEPD media. The lethal effect of toxin-containing supernatant and the effect of aeration upon functional killer production and the correlation between the products of anaerobic metabolism and the functional toxin formation were evaluated. The results showed that at low sugar concentration, the toxin of the killer strain of Sacch. cerevisiae was only produced under anaerobic conditions . The system of killer protein production showed to be regulated by Pasteur and Crabtree effects. As soon as the ethanol was formed, the functional killer toxin was produced. The synthesis of the active killer toxin seemed to be somewhat associated with the switch to fermentation process and with concomitant alcohol dehydrogenase (ADH activity.

  11. Effect of production conditions on the stability of a human bifidobacterial species Bifidobacterium longum in yogurt.

    Science.gov (United States)

    Abe, F; Tomita, S; Yaeshima, T; Iwatsuki, K

    2009-12-01

    Human bifidobacteria are more sensitive to external environmental factors than animal bifidobacteria, and it is difficult to ensure their stable survival in yogurt. The purpose of this investigation was to observe the survival of human bifidobacteria in yogurts produced under various production conditions. Frozen or lyophilized bifidobacteria starters containing Bifidobacterium longum BB536 originally isolated from an infant, and commercial lyophilized yogurt starters were used for yogurt preparation. After producing yogurts under various conditions, the survival of bifidobacteria in these yogurts over various storage periods was observed. Although there were some differences in bifidobacterial survival in yogurt between various production conditions, more than 1.0 x 10(7) CFU g(-1) of Bif. longum survived in yogurt after 35 days' storage at 5 degrees C. Lower fermentation temperature (37 degrees C) and inclusion of Lactococcus lactis in the starter significantly (P yogurt. In this investigation, the human bifidobacterial strain Bif. longum survived adequately in yogurt, although the fermentation temperature and starter composition affect bifidobacterial survival. This investigation indicates that stable probiotic yogurt using human bifidobacteria can be produced by choosing optimal production conditions.

  12. Nitrous oxide production during nitrification from organic solid waste under temperature and oxygen conditions.

    Science.gov (United States)

    Nag, Mitali; Shimaoka, Takayuki; Komiya, Teppei

    2016-11-01

    Landfill aeration can accelerate the biological degradation of organic waste and reduce methane production; however, it induces nitrous oxide (N2O), a potent greenhouse gas. Nitrification is one of the pathways of N2O generation as a by-product during aerobic condition. This study was initiated to demonstrate the features of N2O production rate from organic solid waste during nitrification under three different temperatures (20°C, 30°C, and 40°C) and three oxygen concentrations (5%, 10%, and 20%) with high moisture content and high substrates' concentration. The experiment was carried out by batch experiment using Erlenmeyer flasks incubated in a shaking water bath for 72 h. A duplicate experiment was carried out in parallel, with addition of 100 Pa of acetylene as a nitrification inhibitor, to investigate nitrifiers' contribution to N2O production. The production rate of N2O ranged between 0.40 × 10(-3) and 1.14 × 10(-3) mg N/g-DM/h under the experimental conditions of this study. The rate of N2O production at 40°C was higher than at 20°C and 30°C. Nitrification was found to be the dominant pathway of N2O production. It was evaluated that optimization of O2 content is one of the crucial parameters in N2O production that may help to minimize greenhouse gas emissions and N turnover during aeration.

  13. Heterologous production of Pseudomonas aeruginosa rhamnolipid under anaerobic conditions for microbial enhanced oil recovery.

    Science.gov (United States)

    Zhao, F; Shi, R; Zhao, J; Li, G; Bai, X; Han, S; Zhang, Y

    2015-02-01

    The ex situ application of rhamnolipid to enhance oil recovery is costly and complex in terms of rhamnolipid production and transportation, while in situ production of rhamnolipid is restricted by the oxygen-deficient environments of oil reservoirs. To overcome the oxygen-limiting conditions and to circumvent the complex regulation of rhamnolipid biosynthesis in Pseudomonas aeruginosa, an engineered strain Pseudomonas stutzeri Rhl was constructed for heterologous production of rhamnolipid under anaerobic conditions. The rhlABRI genes for rhamnolipid biosynthesis were cloned into a facultative anaerobic strain Ps. stutzeri DQ1 to construct the engineered strain Rhl. Anaerobic production of rhamnolipid was confirmed by thin layer chromatography and Fourier transform infrared analysis. Rhamnolipid product reduced the air-water surface tension to 30.3 mN m(-1) and the oil-water interfacial tension to 0.169 mN m(-1). Rhl produced rhamnolipid of 1.61 g l(-1) using glycerol as the carbon source. Rhl anaerobic culture emulsified crude oil up to EI24 ≈ 74. An extra 9.8% of original crude oil was displaced by Rhl in the core flooding test. Strain Rhl achieved anaerobic production of rhamnolipid and worked well for enhanced oil recovery in the core flooding model. The rhamnolipid produced by Rhl was similar to that of the donor strain SQ6. This is the first study to achieve anaerobic and heterologous production of rhamnolipid. Results demonstrated the potential feasibility of Rhl as a promising strain to enhance oil recovery through anaerobic production of rhamnolipid. © 2014 The Society for Applied Microbiology.

  14. Husbandry factors and health conditions influencing the productivity of French rabbit farms

    Directory of Open Access Journals (Sweden)

    A. Huneau-Salaün

    2015-03-01

    Full Text Available In 2009 productivity data from 95 kindling to finishing rabbit farms in France were analysed to identify rearing factors and health conditions that influenced their productivity. Farm productivity, expressed on a yearly basis, was described with 4 productivity indices: doe fertility and prolificacy, viability of young rabbits in the nest and mortality during the fattening period. The productivity data were obtained with the technical support of the farm and expressed in a standardised way. The average numerical productivity observed in the sample of farms was 50.9 rabbits produced per doe and per year (CI95% [49.6-52.2]. The husbandry management and health conditions were described based on a questionnaire filled out during an interview with the farmer and a farm visit. Explanatory data were organised into meaningful blocks relative to biosecurity measures, del using a Partiamaternity management, the sanitary context and the farm structure. The relationship between the 4 thematic blocks and the productivity indices was studied in a single mol Least Squares (PLS regression model. Fertility (81.0%, CI95% [80.0-82.0] and viability of young at nest (85.1%, CI95% [85.0-85.3] and mortality rate during fattening: 7.2%, CI95% [6.4-7.9] were significantly associated with common factors relative to maternity management and the health context whereas prolificacy (9.7 live kits per parturition, CI95% [9.5-9.9] was mostly influenced by a specific set of variables pertaining to those 2 blocks. Farm structure and biosecurity measures had a limited impact on fertility and on kit viability before weaning. The health conditions of the doe herd and the fattening rabbits were found to be significantly associated with several productivity indexes, but their impacts on productivity were as high as the impact of the other blocks. Genetic strain of the females, doe replacement strategy and nursing and weaning practices appeared to significantly influence reproductive

  15. Hierarchical Satellite-based Approach to Global Monitoring of Crop Condition and Food Production

    Science.gov (United States)

    Zheng, Y.; Wu, B.; Gommes, R.; Zhang, M.; Zhang, N.; Zeng, H.; Zou, W.; Yan, N.

    2014-12-01

    The assessment of global food security goes beyond the mere estimate of crop production: It needs to take into account the spatial and temporal patterns of food availability, as well as physical and economic access. Accurate and timely information is essential to both food producers and consumers. Taking advantage of multiple new remote sensing data sources, especially from Chinese satellites, such as FY-2/3A, HJ-1 CCD, CropWatch has expanded the scope of its international analyses through the development of new indicators and an upgraded operational methodology. The new monitoring approach adopts a hierarchical system covering four spatial levels of detail: global (sixty-five Monitoring and Reporting Units, MRU), seven major production zones (MPZ), thirty-one key countries (including China) and "sub- countries." The thirty-one countries encompass more that 80% of both global exports and production of four major crops (maize, rice, soybean and wheat). The methodology resorts to climatic and remote sensing indicators at different scales, using the integrated information to assess global, regional, and national (as well as sub-national) crop environmental condition, crop condition, drought, production, and agricultural trends. The climatic indicators for rainfall, temperature, photosynthetically active radiation (PAR) as well as potential biomass are first analysed at global scale to describe overall crop growing conditions. At MPZ scale, the key indicators pay more attention to crops and include Vegetation health index (VHI), Vegetation condition index (VCI), Cropped arable land fraction (CALF) as well as Cropping intensity (CI). Together, they characterise agricultural patterns, farming intensity and stress. CropWatch carries out detailed crop condition analyses for thirty one individual countries at the national scale with a comprehensive array of variables and indicators. The Normalized difference vegetation index (NDVI), cropped areas and crop condition are

  16. Fission Product Transport Models Adopted in REFPAC Code for LOCA Conditions in PWR and WWER NPPS

    International Nuclear Information System (INIS)

    Strupczewski, A.

    2003-01-01

    The report presents assumptions and physical models used for calculations of fission product releases from nuclear reactors, their behavior inside the containment and leakages to the environment after large break loss of coolant accident LB LOCA. They are the basis of code REFPAC (RElease of Fission Products under Accident Conditions), designed primarily to represent significant physical processes occurring after LB LOCA. The code describes these processes using three different models. Model 1 corresponds to established US and Russian practice, Model 2 includes all conservative assumptions that are in agreement with the actual state-of-the-art, and Model 3 incorporates formulae and parameter values actually used in EU practice. (author)

  17. Characterization and chemistry of fission products released from LWR fuel under accident conditions

    International Nuclear Information System (INIS)

    Norwood, K.S.; Collins, J.L.; Osborne, M.F.; Lorenz, R.A.; Wichner, R.P.

    1984-01-01

    Segments from commercial LWR fuel rods have been tested at temperatures between 1400 and 2000 0 C in a flowing steam-helium atmosphere to simulate severe accident conditions. The primary goals of the tests were to determine the rate of fission product release and to characterize the chemical behavior. This paper is concerned primarily with the identification and chemical behavior of the released fission products with emphasis on antimony, cesium, iodine, and silver. The iodine appeared to behave primarily as cesium iodide and the antimony and silver as elements, while cesium behavior was much more complex. 17 refs., 7 figs., 1 tab

  18. Life?cycle impacts of ethanol production from spruce wood chips under high-gravity conditions

    OpenAIRE

    Janssen, Matty; Xiros, Charilaos; Tillman, Anne-Marie

    2016-01-01

    Background Development of more sustainable biofuel production processes is ongoing, and technology to run these processes at a high dry matter content, also called high-gravity conditions, is one option. This paper presents the results of a life?cycle assessment (LCA) of such a technology currently in development for the production of bio-ethanol from spruce wood chips. Results The cradle-to-gate LCA used lab results from a set of 30 experiments (or process configurations) in which the main p...

  19. Production of radioactive sodium under working conditions for investigation into petroleum boreholes

    International Nuclear Information System (INIS)

    Kostin, Yu.I.; Gulin, Yu.A.; Sokolov, Yu.I.; Zhuvagin, I.G.

    1976-01-01

    Because it is advisable to use short-lived isotopes in monitoring the condition of boreholes, procedures are proposed for preparing 0.1-0.5 mC of Na 24 (half-life is 14.9 hr.) during a cycle under production conditions. It is recommended that baking soda should be used as activation material and an ampule polonium and beryllium neutron source with an activity of about 15 to 20 mC should be used as a source. Each operation uses 80 kg of baking soda and the activation lasts 30 to 50 hr

  20. On the conditions of magma mixing and its bearing on andesite production in the crust.

    Science.gov (United States)

    Laumonier, Mickael; Scaillet, Bruno; Pichavant, Michel; Champallier, Rémi; Andujar, Joan; Arbaret, Laurent

    2014-12-15

    Mixing between magmas is thought to affect a variety of processes, from the growth of continental crust to the triggering of volcanic eruptions, but its thermophysical viability remains unclear. Here, by using high-pressure mixing experiments and thermal calculations, we show that hybridization during single-intrusive events requires injection of high proportions of the replenishing magma during short periods, producing magmas with 55-58 wt% SiO2 when the mafic end-member is basaltic. High strain rates and gas-rich conditions may produce more felsic hybrids. The incremental growth of crustal reservoirs limits the production of hybrids to the waning stage of pluton assembly and to small portions of it. Large-scale mixing appears to be more efficient at lower crustal conditions, but requires higher proportions of mafic melt, producing more mafic hybrids than in shallow reservoirs. Altogether, our results show that hybrid arc magmas correspond to periods of enhanced magma production at depth.

  1. Diatom production in the marine environment : implications for larval fish growth and condition

    DEFF Research Database (Denmark)

    St. John, Michael; Clemmesen, C.; Lund, T.

    2001-01-01

    To test the effects of diatom production on larval fish growth and condition. laboratory experiments were performed with larval North Sea cod reared on different algal food chains. These food chains were based on cultures of (a) the diatoms Skeletonema costatum and Thalassiosira weissflogii: (b....../omega6 fatty acids in the algal source had no significant effect. The highest and lowest growth rates were observed in food chains based on H. triquetra and T. weissflogii. respectively (means for days 14-16 of 4.0 and - 4.7). The mixed diatom/dinoflagellate diet resulted in inter- mediate growth rates...... and condition. Regressions of growth rates against EPA and DHA content indicated no inhibitory effect of diatom production on growth in larval cod...

  2. Topological and Energetic Conditions for Lithographic Production of Carbon Nanotubes from Graphene

    Directory of Open Access Journals (Sweden)

    D. Fülep

    2015-01-01

    Full Text Available Density Functional Based Tight-Binding (DFTB molecular dynamics (MD simulations were performed for producing carbon nanotubes from graphene nanoribbons. The constant temperature simulations were controlled with the help of Nosé-Hoover thermostat. In our systematic study we obtained critical curvature energies and determined topological conditions for nanotube production from two parallel graphene nanoribbons. We obtained linear relationship between the curvature energy and the square of the curvature.

  3. Vanillin production using metabolically engineered Escherichia coli under non-growing conditions.

    Science.gov (United States)

    Barghini, Paolo; Di Gioia, Diana; Fava, Fabio; Ruzzi, Maurizio

    2007-04-16

    Vanillin is one of the most important aromatic flavour compounds used in the food and cosmetic industries. Natural vanillin is extracted from vanilla beans and is relatively expensive. Moreover, the consumer demand for natural vanillin highly exceeds the amount of vanillin extracted by plant sources. This has led to the investigation of other routes to obtain this flavour such as the biotechnological production from ferulic acid. Studies concerning the use of engineered recombinant Escherichia coli cells as biocatalysts for vanillin production are described in the literature, but yield optimization and biotransformation conditions have not been investigated in details. Effect of plasmid copy number in metabolic engineering of E. coli for the synthesis of vanillin has been evaluated by the use of genes encoding feruloyl-CoA synthetase and feruloyl hydratase/aldolase from Pseudomonas fluorescens BF13. The higher vanillin production yield was obtained using resting cells of E. coli strain JM109 harbouring a low-copy number vector and a promoter exhibiting a low activity to drive the expression of the catabolic genes. Optimization of the bioconversion of ferulic acid to vanillin was accomplished by a response surface methodology. The experimental conditions that allowed us to obtain high values for response functions were 3.3 mM ferulic acid and 4.5 g/L of biomass, with a yield of 70.6% and specific productivity of 5.9 micromoles/g x min after 3 hours of incubation. The final concentration of vanillin in the medium was increased up to 3.5 mM after a 6-hour incubation by sequential spiking of 1.1 mM ferulic acid. The resting cells could be reused up to four times maintaining the production yield levels over 50%, thus increasing three times the vanillin obtained per gram of biomass. Ferulic acid can be efficiently converted to vanillin, without accumulation of undesirable vanillin reduction/oxidation products, using E. coli JM109 cells expressing genes from the ferulic

  4. Vanillin production using metabolically engineered Escherichia coli under non-growing conditions

    Directory of Open Access Journals (Sweden)

    Fava Fabio

    2007-04-01

    Full Text Available Abstract Background Vanillin is one of the most important aromatic flavour compounds used in the food and cosmetic industries. Natural vanillin is extracted from vanilla beans and is relatively expensive. Moreover, the consumer demand for natural vanillin highly exceeds the amount of vanillin extracted by plant sources. This has led to the investigation of other routes to obtain this flavour such as the biotechnological production from ferulic acid. Studies concerning the use of engineered recombinant Escherichia coli cells as biocatalysts for vanillin production are described in the literature, but yield optimization and biotransformation conditions have not been investigated in details. Results Effect of plasmid copy number in metabolic engineering of E. coli for the synthesis of vanillin has been evaluated by the use of genes encoding feruloyl-CoA synthetase and feruloyl hydratase/aldolase from Pseudomonas fluorescens BF13. The higher vanillin production yield was obtained using resting cells of E. coli strain JM109 harbouring a low-copy number vector and a promoter exhibiting a low activity to drive the expression of the catabolic genes. Optimization of the bioconversion of ferulic acid to vanillin was accomplished by a response surface methodology. The experimental conditions that allowed us to obtain high values for response functions were 3.3 mM ferulic acid and 4.5 g/L of biomass, with a yield of 70.6% and specific productivity of 5.9 μmoles/g × min after 3 hours of incubation. The final concentration of vanillin in the medium was increased up to 3.5 mM after a 6-hour incubation by sequential spiking of 1.1 mM ferulic acid. The resting cells could be reused up to four times maintaining the production yield levels over 50%, thus increasing three times the vanillin obtained per gram of biomass. Conclusion Ferulic acid can be efficiently converted to vanillin, without accumulation of undesirable vanillin reduction/oxidation products

  5. Continuous aryl alcohol oxidase production under growth-limited conditions using a trickle bed reactor.

    Science.gov (United States)

    Pardo-Planas, Oscar; Atiyeh, Hasan K; Prade, Rolf A; Müller, Michael; Wilkins, Mark R

    2018-05-01

    An A. nidulans strain with a pyridoxine marker was used for continuous production of aryl alcohol oxidase (AAO) in a trickle bed reactor (TBR). Modified medium with reduced zinc, no copper, and 5 g/L ascorbic acid that reduced melanin production and increased AAO productivity under growth limited conditions was used. Two air flow rates, 0.11 L/min (0.1 vvm) and 1.1 L/min (1.0 vvm) were tested. More melanin formation and reduced protein productivity were observed with air flow rate of 1.1 L/min. Three random packings were used as support for the fungus inside the TBR column, two of which were hydrophobic and one which was hydrophilic, and three different dilution rates were tested. The use of GEA BCN 030 hydrophobic packing resulted in greater AAO yield and productivity than the other packings. Increasing dilution rates favored melanin formation and citric, lactic and succinic acid accumulation, which decreased AAO yield and productivity. Copyright © 2018 Elsevier Ltd. All rights reserved.

  6. Effects Disposal Condition and Ground Water to Leaching Rate of Radionuclides from Solidification Products

    International Nuclear Information System (INIS)

    Herlan Martono; Wati

    2008-01-01

    Effects disposal condition and ground water to leaching rate of radionuclides from solidification products have been studied. The aims of leaching test at laboratory to get the best composition of solidified products for continuous process or handling. The leaching rate of radionuclides from the many kinds of matrix from smallest to bigger are glass, thermosetting plastic, urea formaldehyde, asphalt, and cement. Glass for solidification of high level waste, thermosetting plastic and urea formaldehyde for solidification of low and intermediate waste, asphalt and cement for solidification of low and intermediate level waste. In shallow land burial, ground water rate is fast, debit is high, and high permeability, so the probability contact between solidification products and ground water is occur. The pH of ground water increasing leaching rate, but cation in the ground water retard leaching rate. Effects temperature radiation and radiolysis to solidification products is not occur. In the deep repository, ground water rate is slow, debit is small, and low permeability, so the probability contact between solidification products and ground water is very small. There are effect cooling time and distance between pits to rock temperature. Alfa radiation effects can be occur, but there is no contact between solidification products and ground water, so that there is not radiolysis. (author)

  7. Petunia × hybrida floral scent production is negatively affected by high-temperature growth conditions.

    Science.gov (United States)

    Cna'ani, Alon; Mühlemann, Joelle K; Ravid, Jasmin; Masci, Tania; Klempien, Antje; Nguyen, Thuong T H; Dudareva, Natalia; Pichersky, Eran; Vainstein, Alexander

    2015-07-01

    Increasing temperatures due to changing global climate are interfering with plant-pollinator mutualism, an interaction facilitated mainly by floral colour and scent. Gas chromatography-mass spectroscopy analyses revealed that increasing ambient temperature leads to a decrease in phenylpropanoid-based floral scent production in two Petunia × hybrida varieties, P720 and Blue Spark, acclimated at 22/16 or 28/22 °C (day/night). This decrease could be attributed to down-regulation of scent-related structural gene expression from both phenylpropanoid and shikimate pathways, and up-regulation of a negative regulator of scent production, emission of benzenoids V (EOBV). To test whether the negative effect of increased temperature on scent production can be reduced in flowers with enhanced metabolic flow in the phenylpropanoid pathway, we analysed floral volatile production by transgenic 'Blue Spark' plants overexpressing CaMV 35S-driven Arabidopsis thaliana production of anthocyanin pigments 1 (PAP1) under elevated versus standard temperature conditions. Flowers of 35S:PAP1 transgenic plants produced the same or even higher levels of volatiles when exposed to a long-term high-temperature regime. This phenotype was also evident when analysing relevant gene expression as inferred from sequencing the transcriptome of 35S:PAP1 transgenic flowers under the two temperature regimes. Thus, up-regulation of transcription might negate the adverse effects of temperature on scent production. © 2014 John Wiley & Sons Ltd.

  8. Growth, photosynthetic pigments and production of essential oil of long-pepper under different light conditions.

    Science.gov (United States)

    Lima, Vandimilli A; Pacheco, Fernanda V; Avelar, Rafaella P; Alvarenga, Ivan C A; Pinto, José Eduardo B P; Alvarenga, Amauri A DE

    2017-01-01

    Piper hispidinervum C. DC. is popularly known as long-pepper and it owns a commercial value due to the essential oil it produces. Long-pepper oil is rich in safrole and eugenoln components that have insecticidal, fungicidal and bactericidal activity. It has been establish that to medicinal plants light influences not only growth but also essential oil production. The growth, the content of photosynthetic pigments and the essential oil production of Piper hispidinervum at greenhouses with different light conditions was evaluated. The treatments were characterized by cultivation of plants for 180 days under different light conditions, produced by shading greenhouses with 50% and 30% of natural incident irradiance, two colored shading nets red (RN) and blue (BN) both blocking 50% of the incident radiation and one treatment at full-sun (0% of shade). The results showed that the treatments of 50% shade and RN and BN were the ones which stimulated the greater growth. Blue and red light also had the best production of photosynthetic pigments. Essential oil yielded more under full sun therefore this is the most indicated condition to produce seedlings for the chemical and pharmaceutical industry.

  9. Growth, photosynthetic pigments and production of essential oil of long-pepper under different light conditions

    Directory of Open Access Journals (Sweden)

    VANDIMILLI A. LIMA

    Full Text Available ABSTRACT Piper hispidinervum C. DC. is popularly known as long-pepper and it owns a commercial value due to the essential oil it produces. Long-pepper oil is rich in safrole and eugenoln components that have insecticidal, fungicidal and bactericidal activity. It has been establish that to medicinal plants light influences not only growth but also essential oil production. The growth, the content of photosynthetic pigments and the essential oil production of Piper hispidinervum at greenhouses with different light conditions was evaluated. The treatments were characterized by cultivation of plants for 180 days under different light conditions, produced by shading greenhouses with 50% and 30% of natural incident irradiance, two colored shading nets red (RN and blue (BN both blocking 50% of the incident radiation and one treatment at full-sun (0% of shade. The results showed that the treatments of 50% shade and RN and BN were the ones which stimulated the greater growth. Blue and red light also had the best production of photosynthetic pigments. Essential oil yielded more under full sun therefore this is the most indicated condition to produce seedlings for the chemical and pharmaceutical industry.

  10. Quantitative Analysis of Existing Conditions and Production Strategies for the Baca Geothermal System, New Mexico

    Science.gov (United States)

    Faust, Charles R.; Mercer, James W.; Thomas, Stephen D.; Balleau, W. Pete

    1984-05-01

    The Baca geothermal reservoir and adjacent aquifers in the Jemez Mountains of New Mexico comprise an integrated hydrogeologic system. Analysis of the geothermal reservoir either under natural conditions or subject to proposed development should account for the mass (water) and energy (heat) balances of adjacent aquifers as well as the reservoir itself. A three-dimensional model based on finite difference approximations is applied to this integrated system. The model simulates heat transport associated with the flow of steam and water through an equivalent porous medium. The Baca geothermal reservoir is dominated by flow in fractures and distinct strata, but at the scale of application the equivalent porous media concept is appropriate. The geothermal reservoir and adjacent aquifers are simulated under both natural conditions and proposed production strategies. Simulation of natural conditions compares favorably with observed pressure, temperature, and thermal discharge data. The history matching simulations show that the results used for comparison are most sensitive to vertical permeability and the area of an assumed high-permeability zone connecting the reservoir to a deep hydrothermal source. Simulations using proposed production strategies and optimistic estimates of certain hydrologic parameters and reservoir extent indicate that a 50-MW power plant could be maintained for a period greater than 30 years. This production, however, will result in significant decreases in the total water discharge to the Jemez River.

  11. Production of native arbuscular mycorrhizal fungi inoculum under different environmental conditions

    Directory of Open Access Journals (Sweden)

    Yamir Torres-Arias

    Full Text Available Abstract In order to obtain an arbuscular mycorrhizal fungi (AMF native inoculum from Sierra de Moa and determine the most appropriate conditions for its big scale production, four light and temperature combinations were tested in three plant species (Calophyllum antillanum, Talipariti elatum and Paspalum notatum. Growth and development parameters, as well as the mycorrhizal functioning of the seedlings were evaluated. The natural light treatment under high temperatures (L-H was the most suitable for the growth and development of the three plant species, showing the highest total biomass values, mainly of root, and a positive root-shoot ratio balance. This treatment also promoted higher values of root mycorrhizal colonization, external mycelium and AMF spore density. A total of 38 AMF species were identified among the plants and environmental conditions tested. Archaeospora sp.1, Glomus sp.5, Glomus brohultii and G. glomerulatum were observed in all the treatments. The L-H condition can be recommended for native inoculum production, as it promotes a better expression of the AM symbiosis and an elevated production of mycorrhizal propagules.

  12. Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions

    Directory of Open Access Journals (Sweden)

    Bhattacharyya Anamitra

    2007-06-01

    Full Text Available Abstract Background It has become evident that host cells react to recombinant protein production with a variety of metabolic and intrinsic stresses such as the unfolded protein response (UPR pathway. Additionally, environmental conditions such as growth temperature may have a strong impact on cell physiology and specific productivity. However, there is little information about the molecular reactions of the host cells on a genomic level, especially in context to recombinant protein secretion. For the first time, we monitored transcriptional regulation of a subset of marker genes in the common production host Pichia pastoris to gain insights into the general physiological status of the cells under protein production conditions, with the main focus on secretion stress related genes. Results Overexpression of the UPR activating transcription factor Hac1p was employed to identify UPR target genes in P. pastoris and the responses were compared to those known for Saccharomyces cerevisiae. Most of the folding/secretion related genes showed similar regulation patterns in both yeasts, whereas genes associated with the general stress response were differentially regulated. Secretion of an antibody Fab fragment led to induction of UPR target genes in P. pastoris, however not to the same magnitude as Hac1p overproduction. Overexpression of S. cerevisiae protein disulfide isomerase (PDI1 enhances Fab secretion rates 1.9 fold, but did not relief UPR stress. Reduction of cultivation temperature from 25°C to 20°C led to a 1.4-fold increase of specific product secretion rate in chemostat cultivations, although the transcriptional levels of the product genes (Fab light and heavy chain were significantly reduced at the lower temperature. A subset of folding related genes appeared to be down-regulated at the reduced temperature, whereas transcription of components of the ER associated degradation and the secretory transport was enhanced. Conclusion Monitoring of

  13. Force production during squats performed with a rotational resistance device under stable versus unstable conditions.

    Science.gov (United States)

    Moras, Gerard; Vázquez-Guerrero, Jairo

    2015-11-01

    [Purpose] Force production during a squat action on a rotational resistance device (RRD) under stable and unstable conditions. [Subjects and Methods] Twenty-one healthy males were asked to perform six sets of six repetitions of squats on an RRD on either stable or unstable surfaces. The stable and unstable sets were performed on different days. Muscular outputs were obtained from a linear encoder and a strain gauge fixed to a vest. [Results] Overall, the results showed no significant differences for any of the dependent variables across exercise modes. Forcemean outputs were higher in the concentric phase than in the eccentric phase for each condition, but there were no differences in velocity, time or displacement. The forcepeak was similar in the eccentric and concentric phases of movement under both stable and unstable conditions. There were no significant differences in forcemean between sets per condition or between conditions. [Conclusion] These results suggest that performing squats with a RRD achieves similar forcemean and forcepeak under stable and unstable conditions. The forcepeak produced is also similar in concentric and eccentric phases.

  14. Lean production tools and decision latitude enable conditions for innovative learning in organizations: a multilevel analysis.

    Science.gov (United States)

    Fagerlind Ståhl, Anna-Carin; Gustavsson, Maria; Karlsson, Nadine; Johansson, Gun; Ekberg, Kerstin

    2015-03-01

    The effect of lean production on conditions for learning is debated. This study aimed to investigate how tools inspired by lean production (standardization, resource reduction, visual monitoring, housekeeping, value flow analysis) were associated with an innovative learning climate and with collective dispersion of ideas in organizations, and whether decision latitude contributed to these associations. A questionnaire was sent out to employees in public, private, production and service organizations (n = 4442). Multilevel linear regression analyses were used. Use of lean tools and decision latitude were positively associated with an innovative learning climate and collective dispersion of ideas. A low degree of decision latitude was a modifier in the association to collective dispersion of ideas. Lean tools can enable shared understanding and collective spreading of ideas, needed for the development of work processes, especially when decision latitude is low. Value flow analysis played a pivotal role in the associations. Copyright © 2014 Elsevier Ltd and The Ergonomics Society. All rights reserved.

  15. Silicification-induced cell aggregation for the sustainable production of H2 under aerobic conditions.

    Science.gov (United States)

    Xiong, Wei; Zhao, Xiaohong; Zhu, Genxing; Shao, Changyu; Li, Yaling; Ma, Weimin; Xu, Xurong; Tang, Ruikang

    2015-10-05

    Photobiological hydrogen production is of great importance because of its promise for generating clean renewable energy. In nature, green algae cannot produce hydrogen as a result of the extreme sensitivity of hydrogenase to oxygen. However, we find that silicification-induced green algae aggregates can achieve sustainable photobiological hydrogen production even under natural aerobic conditions. The core-shell structure of the green algae aggregates creates a balance between photosynthetic electron generation and hydrogenase activity, thus allowing the production of hydrogen. This finding provides a viable pathway for the solar-driven splitting of water into hydrogen and oxygen to develop green energy alternatives by using rationally designed cell-material complexes. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Probiotics production and alternative encapsulation methodologies to improve their viabilities under adverse environmental conditions.

    Science.gov (United States)

    Coghetto, Chaline Caren; Brinques, Graziela Brusch; Ayub, Marco Antônio Záchia

    2016-12-01

    Probiotic products are dietary supplements containing live microorganisms producing beneficial health effects on the host by improving intestinal balance and nutrient absorption. Among probiotic microorganisms, those classified as lactic acid bacteria are of major importance to the food and feed industries. Probiotic cells can be produced using alternative carbon and nitrogen sources, such as agroindustrial residues, at the same time contributing to reduce process costs. On the other hand, the survival of probiotic cells in formulated food products, as well as in the host gut, is an essential nutritional aspect concerning health benefits. Therefore, several cell microencapsulation techniques have been investigated as a way to improve cell viability and survival under adverse environmental conditions, such as the gastrointestinal milieu of hosts. In this review, different aspects of probiotic cells and technologies of their related products are discussed, including formulation of culture media, and aspects of cell microencapsulation techniques required to improve their survival in the host.

  17. Vaporization of low-volatile fission products under severe CANDU reactor accident conditions

    International Nuclear Information System (INIS)

    Lewis, B.J.; Corse, B.J.; Thompson, W.T.; Kaye, M.H.; Iglesias, F.C.; Elder, P.; Dickson, R.; Liu, Z.

    1997-01-01

    An analytical model has been developed to describe the release behaviour of low-volatile fission products from uranium dioxide fuel under severe reactor accident conditions. The effect of the oxygen potential on the chemical form and volatility of fission products is determined by Gibbs-energy minimization. The release kinetics are calculated according to the rate-controlling step of diffusional transport in the fuel matrix or fission product vaporization from the fuel surface. The effect of fuel volatilization (i.e., matrix stripping) on the release behaviour is also considered. The model has been compared to data from an out-of-pile annealing experiment performed in steam at the Chalk River Laboratories. (author)

  18. Evaluation of Culture Conditions for Tannase Production by Aspergillus niger GH1

    Directory of Open Access Journals (Sweden)

    Mario Cruz-Hernández

    2006-01-01

    Full Text Available Extra- and intracellular tannase production by Aspergillus niger GH1 has been evaluated using submerged (SmF and solid-state fermentation (SSF at different temperatures (30, 40 and 50 °C. Effects of initial substrate (tannic acid concentration, incubation time and temperature on tannase production in SSF have been studied. A. niger GH1 produced the highest tannase level (2291 U/L in SSF at 30 °C during the first 20 h of culture at tannic acid concentration of 50 g/L, and under these conditions enzyme production was entirely extracellular. The decline in tannase activity after 20 h of incubation was associated with a concomitant increase in protease activity.

  19. THE EMPLOYMENT – PRODUCTIVITY – SALARY RELATION, A CONDITION FOR ACHIEVING LONG - TERM ECONOMIC GROWTH

    Directory of Open Access Journals (Sweden)

    Mihai CHIRILA

    2005-01-01

    Full Text Available The employment-productivity-salary correlation is essential towards setting up conditions for economic growth andlasting social stability. An influence exists from technological changes, current stage of economic development,peculiarities of labour offer, employers – trade-unions interaction, etc. The analysis covers long-term and short-termcycles. In keeping with specificity of development stages, national economy witnesses an interval of productivitygrowth facilitating maximized employment and a rise in salary too. A lower growth rate indicates difficulties inassimilating new technologies; a higher growth rate generates instability in employing labour. Thus, the exercise ofstrong pressure is shown to exist on economic and technological structures. Likewise, this is what happens whenproductivity goes ahead of the separate analyses of the correlations between employment and productivity, on theone hand, and productivity and salary, on the other.

  20. The optimal conditions for nata production from sugar palm syrup by Acetobacter xylinum TISTR 107

    Directory of Open Access Journals (Sweden)

    Laochareonsuk, T.

    2005-11-01

    Full Text Available The optimal conditions of nata production from the fermentation of sugar palm syrup by Acetobacter xylinum TISTR 107 was studied. The results showed that optimized production for a litre of sugar palm syrup medium should compose 15 ºBrix concentration, 7.0 g NH4H2PO4 and 0.7 g MgSO4. 7 H2O at pH 4.25 and incubation at room temperature. The thickness of nata production reached 1.15 cm in 9 days. Sensory evaluation showed that there were no significant difference in odor and acceptability between the nata from sugar palm syrup and the traditional nata production from coconut juice whereas there were significant differences in color and texture. However, the nata from sugar palm syrup gave a better texture. Chemical analysis of the nata produced under these optimal culture conditions revealed 0.13% protein, 0.012% fat, 2.74% fiber, 0.378% nitrogen-free extract, 0.11% ash and 96.63% moisture content. The results suggest that nata produced from sugar palm syrup can be used in food and confectionery.

  1. Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production.

    Science.gov (United States)

    Posada-Uribe, Luisa F; Romero-Tabarez, Magally; Villegas-Escobar, Valeska

    2015-10-01

    Bacillus subtilis spores have important biotechnological applications; however, achieving both, high spore cell densities and sporulation efficiencies in fermentation, is poorly reported. In this study, medium components and culture conditions were optimized with different statistical methods to increase spore production of the plant growth promoting rhizobacteria B. subtilis EA-CB0575. Key medium components were determined with Plackett-Burman (PB) design, and the optimum concentration levels of two components (glucose, MgSO4·7H2O) were optimized with a full factorial and central composite design, achieving 1.37 × 10(9) CFU/mL of spore cell density and 93.5 % of sporulation efficiency in shake flask. The optimized medium was used to determine the effect of culture conditions on spore production at bioreactor level, finding that maintaining pH control did not affect significantly spore production, while the interaction of agitation and aeration rates had a significant effect on spore cell density. The overall optimization generated a 17.2-fold increase in spore cell density (8.78 × 10(9) CFU/mL) and 1.9-fold increase in sporulation efficiency (94.2 %) compared to that of PB design. These results indicate the potential of B. subtilis EA-CB0575 to produce both, high spore cell densities and sporulation efficiencies, with very low nutrient requirements and short incubation period which can represent savings of process production.

  2. Optimization of culture condition for ACEI and GABA production by lactic acid bacteria.

    Science.gov (United States)

    Tung, Yi-Ting; Lee, Bao-Hong; Liu, Chin-Feng; Pan, Tzu-Ming

    2011-01-01

    Gamma-aminobutyric acid (GABA) and angiotensin-converting enzyme inhibitor (ACEI) are compounds which can influence hypertension. The goal of this study is to optimize the culture condition for GABA and ACEI production by Lactobacillus plantarum NTU 102 fermented skim milk. In this study, we used 3-factor-3-level Box-Behnken design combining with response surface methodology, where the 3 factors represent the concentration of skim milk, the concentration of monosodium glutamate, and culture temperature. Best conditions for GABA and ACEI production differed. The results indicated that L. plantarum NTU 102 produced the highest combined levels of GABA and ACEI at 37 °C, in milk having 8% to 12% nonfat solids supplemented with 0.6% to 1% MSG. Agitation of the medium during fermentation had no effect on GABA or ACEI production but extended incubation (up to 6 d) increases levels of the bioactive compounds. L. plantarum NTU 102 fermented products may be a potential functional food source for regulating hypertension. © 2011 Institute of Food Technologists®

  3. Assessment of productivity loss in air-conditioned buildings using PMV index

    Energy Technology Data Exchange (ETDEWEB)

    Kosonen, R. [Halton OY, Kausala (Finland); Tan, F. [CapitaLand Commercial Limited, Singapore (Singapore)

    2004-07-01

    This theoretical study reports on the assessment of productivity loss in air-conditioned office buildings using the PMV approach and makes use of Wyon's reviews [D.P. Wyon, P.O. Fanger, B.W. Olesen, C.J.K. Pedersen, The mental performance of subjects clothed for comfort at two different air temperatures, Ergonomics 18 (1975) 358-374; D.P. Wyon, Individual microclimate control: required range, probable benefits and current feasibility, in: Proceedings of Indoor Air '96, Institute of Public Health, Tokyo, 1996; D.P. Wyon, Indoor environmental effects on productivity. IAQ 96 Paths to better building environments/Keynote address. Y. Kevin. Atlanta, ASHRAE, pp. 5-15] as the basis to compare and to relate how the productivity loss could be minimised through improved thermal comfort design criteria. The finding shows that task-related performance is significantly correlated with the human perception of thermal environment that in turn is dependent on temperatures. Different combinations of thermal criteria (air velocity, clo, metabolic, etc.) can lead to similar PMV value and the PMV equation is useful to predict productivity loss that is due to the rate of change in thermal conditions. The study also highlights the issues that remain to be resolved in future research. (author)

  4. Effect of mycorrhizas application on plant growth and nutrient uptake in cucumber production under field conditions

    Energy Technology Data Exchange (ETDEWEB)

    Ortas, I.

    2010-07-01

    Mycorrhizas application in horticultural production in the Eastern Mediterranean region of Turkey has been studied under field conditions for several years. The effects of different arbuscular mycorrhizal fungi (AMF) have been evaluated under field conditions for cucumber production. The parameters measured were seedling survival, plant growth and yield, and root colonization. In 1998 and 1999, Glomus mosseae and Glomus etunicatum inoculated cucumber seedlings were treated with and without P (100 kg P2O5 ha-1) application. A second experiment was set up to evaluate the response of cucumber to the inoculation with a consortia of indigenous mycorrhizae, G. mosseae, G. etunicatum, Glomus clarum, Glomus caledonium and a mixture of these four species. Inoculated and control non inoculated cucumber seedlings were established under field conditions in 1998, 2001, 2002 and 2004. Seedling quality, seedling survival under field conditions and yield response to mycorrhiza were tested. Fruits were harvested periodically; at blossom, plant leaves and root samples were taken for nutrient content and mycorrhizal colonization analysis respectively. The field experiment results showed that mycorrhiza inoculation significantly increased cucumber seedling survival, fruit yield, P and Zn shoot concentrations. Indigenous mycorrhiza inoculum was successful in colonizing plant roots and resulted in better plant growth and yield. The relative effectiveness of each of the inocula tested was not consistent in the different experiments, although inoculated plants always grew better than control no inoculated. The most relevant result for growers was the increased survival of seedlings. (Author) 20 refs.

  5. Optimization of spray drying conditions to microencapsulate cupuassu (Theobroma grandiflorum) seed by-product extract.

    Science.gov (United States)

    da Costa, Russany Silva; Teixeira, Camilo Barroso; Gabbay Alves, Taís Vanessa; Ribeiro-Costa, Roseane M; Casazza, Alessandro A; Aliakbarian, Bahar; Converti, Attilio; Silva Júnior, José O C; Perego, Patrizia

    2018-04-16

    Cupuassu (Theobroma grandiflorum Schum.) is a popular Amazonian fruit because of its intense aroma and nutritional value, whose lipid fraction is alternatively used in cosmetics. To preserve active principles and ensure their controlled release, extract was microencapsulated by spray drying. Influence of spray-drying conditions on microencapsulation of cupuassu seed by-product extract was investigated according to a 3 3 -Box Behnken factorial design, selecting inlet temperature, maltodextrin concentration and feed flowrate as independent variables, and total polyphenol and flavonoid contents, antiradical power, yields of drying and microencapsulation as responses. Fitting the results by second-order equations and modelling by Response Surface Methodology allowed predicting optimum conditions. Epicatechin and glycosylated quercetin were the major microencapsulated flavonoids. Microparticles showed satisfactory antiradical power and stability at 5 °C or under simulated gastrointestinal conditions, thus they may be used to formulate new foods or pharmaceuticals.

  6. Optimization of fermentation conditions for the production of curcumin by engineered Escherichia coli.

    Science.gov (United States)

    Couto, Márcia R; Rodrigues, Joana L; Rodrigues, Lígia R

    2017-08-01

    Curcumin is a plant secondary metabolite with outstanding therapeutic effects. Therefore, there is a great interest in developing new strategies to produce this high-value compound in a cheaper and environmentally friendly way. Curcumin heterologous production in Escherichia coli using artificial biosynthetic pathways was previously demonstrated using synthetic biology approaches. However, the culturing conditions to produce this compound were not optimized and so far only a two-step fermentation process involving the exchange of culture medium allowed high concentrations of curcumin to be obtained, which limits its production at an industrial scale. In this study, the culturing conditions to produce curcumin were evaluated and optimized. In addition, it was concluded that E. coli BL21 allows higher concentrations of curcumin to be produced than E. coli K-12 strains. Different isopropyl β-d-thiogalactopyranoside concentrations, time of protein expression induction and substrate type and concentration were also evaluated. The highest curcumin production obtained was 959.3 µM (95.93% of per cent yield), which was 3.1-fold higher than the highest concentration previously reported. This concentration was obtained using a two-stage fermentation with lysogeny broth (LB) and M9. Moreover, terrific broth was also demonstrated to be a very interesting alternative medium to produce curcumin because it also led to high concentrations (817.7 µM). The use of this single fermentation medium represents an advantage at industrial scale and, although the final production is lower than that obtained with the LB-M9 combination, it leads to a significantly higher production of curcumin in the first 24 h of fermentation. This study allowed obtaining the highest concentrations of curcumin reported so far in a heterologous organism and is of interest for all of those working with the heterologous production of curcuminoids, other complex polyphenolic compounds or plant secondary

  7. Optimization of mass-production conditions for tritium breeder pebbles based on slurry droplet wetting method

    Energy Technology Data Exchange (ETDEWEB)

    Park, Yi-Hyun, E-mail: yhpark@nfri.re.kr [National Fusion Research Institute, Daejeon (Korea, Republic of); Min, Kyung-Mi; Ahn, Mu-Young; Cho, Seungyon; Lee, Young-Min [National Fusion Research Institute, Daejeon (Korea, Republic of); Park, Sang-Jin; Danish, Rehan; Lim, Chul-Hwan; Jo, Yong-Dae [IVT Co., Ltd., Daegu (Korea, Republic of)

    2016-11-01

    Highlights: • An automatic dispensing system was developed to improve uniformity and production rate of breeder pebbles. • The production rate of this system for Li{sub 2}TiO{sub 3} pebble was estimated at 50 kg/year. • The optimization of dispensing and sintering conditions for the mass-production of Li{sub 2}TiO{sub 3} pebble was conducted. • Integrity of Li{sub 2}TiO{sub 3} pebble was able to be ensured during mass-production process, especially during batch process. - Abstract: Lithium metatitanate (Li{sub 2}TiO{sub 3}) is being considered as tritium breeding material for solid-type breeding blanket, which are used in pebble-bed form. The total amount of Li{sub 2}TiO{sub 3} pebbles in Helium Cooled Ceramic Reflector (HCCR) Test Blanket Module (TBM) is approximately 80 kg. Furthermore, DEMO reactor requires a great deal of breeder pebbles. Therefore, the development of mass-production system for breeder pebbles is necessary. The slurry droplet wetting method was adopted in the mass-production process for Li{sub 2}TiO{sub 3} pebbles, which had been developed in Korea. In this method, an automatic slurry dispensing system is one of the key apparatuses because the uniformity of pebbles and production rate are able to be improved. The system was successfully manufactured, which was consisted of a dispensing unit for instillation of Li{sub 2}TiO{sub 3} slurry, a glycerin bath for hardening of droplets, and an automatic maintaining unit for constant distance between syringe needle and glycerin surface. The production rate of this system for Li{sub 2}TiO{sub 3} pebble was estimated at 50 kg/year. In this study, it was investigated that the effect of dispensing and sintering conditions on the mass-production of Li{sub 2}TiO{sub 3} pebbles.

  8. Optimization of culture conditions for gamma-aminobutyric acid production in fermented adzuki bean milk

    Directory of Open Access Journals (Sweden)

    Hung Yi Song

    2018-01-01

    Full Text Available γ-Aminobutyric acid (GABA, a nonprotein amino acid, is widely distributed in nature and fulfills several physiological functions. In this study, various lactic acid strains commonly used to produce fermented milk products were inoculated into adzuki bean milk for producing GABA. The high GABA producing strain was selected in further experiment to improve the GABA production utilizing culture medium optimization. The results demonstrated that adzuki bean milk inoculated with Lactobacillus rhamnosus GG increased GABA content from 0.05 mg/mL to 0.44 mg/mL after 36 hours of fermentation, which showed the greatest elevation in this study. Furthermore, the optimal cultural condition to adzuki bean milk inoculated with L. rhamnosus GG to improve the GABA content was performed using response surface methodology. The results showed that GABA content was dependent on the addition of galactose, monosodium glutamate, and pyridoxine with which the increasing ratios of GABA were 23–38%, 24–68%, and 8–36%, respectively. The optimal culture condition for GABA production of adzuki bean milk was found at the content of 1.44% galactose, 2.27% monosodium glutamate, and 0.20% pyridoxine. Under the optimal cultural condition, the amount of GABA produced in the fermented adzuki bean milk was 1.12 mg/mL, which was 22.4-fold higher than that of the unfermented adzuki bean milk (0.05 mg/100 mL. The results suggested that the optimized cultural condition of adzuki bean milk inoculated with L. rhamnosus GG can increase GABA content for consumers as a daily supplement as suggested.

  9. Crossing Methods and Cultivation Conditions for Rapid Production of Segregating Populations in Three Grain Amaranth Species.

    Science.gov (United States)

    Stetter, Markus G; Zeitler, Leo; Steinhaus, Adrian; Kroener, Karoline; Biljecki, Michelle; Schmid, Karl J

    2016-01-01

    Grain amaranths (Amaranthus spp.) have been cultivated for thousands of years in Central and South America. Their grains are of high nutritional value, but the low yield needs to be increased by selection of superior genotypes from genetically diverse breeding populations. Amaranths are adapted to harsh conditions and can be cultivated on marginal lands although little is known about their physiology. The development of controlled growing conditions and efficient crossing methods is important for research on and improvement of this ancient crop. Grain amaranth was domesticated in the Americas and is highly self-fertilizing with a large inflorescence consisting of thousands of very small flowers. We evaluated three different crossing methods (open pollination, hot water emasculation and hand emasculation) for their efficiency in amaranth and validated them with genetic markers. We identified cultivation conditions that allow an easy control of flowering time by day length manipulation and achieved flowering times of 4 weeks and generation times of 2 months. All three different crossing methods successfully produced hybrid F1 offspring, but with different success rates. Open pollination had the lowest (10%) and hand emasculation the highest success rate (74%). Hot water emasculation showed an intermediate success rate (26%) with a maximum of 94% success. It is simple to perform and suitable for a more large-scale production of hybrids. We further evaluated 11 single nucleotide polymorphism (SNP) markers and found that they were sufficient to validate all crosses of the genotypes used in this study for intra- and interspecific hybridizations. Despite its very small flowers, crosses in amaranth can be carried out efficiently and evaluated with inexpensive SNP markers. Suitable growth conditions strongly reduce the generation time and allow the control of plant height, flowering time, and seed production. In combination, this enables the rapid production of segregating

  10. FRESHWATER FISH FARMING CONDITIONS IN 1999 AND THE PRODUCTION PLAN FOR 2000

    Directory of Open Access Journals (Sweden)

    Zlatko Homen

    2000-09-01

    Full Text Available One of the activities of the Ministry of Agriculture and Forestry, Fishery and Mediterranean Agriculture Directorate, i. e. Fishery Directorate to be more specific, is the monitoring of conditions in freshwater fish farming. The objective of this work is to show conditions in freshwater fish farming during 1999. and to provide a production plan for 2000. It will also provide detailed insight into the present conditions in freshwater fish farming and into the production trends of this field. Regarding this issue, the »Questionnaire for the Monitoring of Conditions in Freshwater Fish Farming«, was sent to fish farmers aroud the country data was processed from 22 cap ponds and 13 trout ponds. Roughe estimates of conditions were conducted for 2 fish farms, since they haven’t yet returned the mandatoruy questionnaire, i. e. the necessary data. This work features data on the number of employees in fish farms, as well as their qualifications, on the actual production and distribution of farmed freshwater fish, on the areas where production was conducted and on the overall yield. Fish food, raw materials, used tools and incentive funds paid were also taken into consideration. The difficulties faced in this branch of the economy are also inicated. Compared to 1998, a slight decrease in the number of employees of freshwater fish farms has been recorded. The total number of employees in 1999 was 655, of which 555 were on carp ponds and 100 on trout ponds. Data on the qualifications of employees for 1999 show that most of them were unskilled workers, while highly skilled workers make up a minority in this work force. The total production of freshwater in 1999. amoounted to 6.185,51 tons. Of this amount 5.592,52 tons were warm-water fish specied and 592,99 tons were cold-water fish species. Compared to 1998, production decreased by 4,89 percent. Production for 2000 has been planned to increase by 22,15 percent, i. e. the production quantity is projected

  11. Economic analysis of sunflower production in the view of orobanche resistance conditions

    International Nuclear Information System (INIS)

    Semerci, A.

    2013-01-01

    The objective of this research is to determine the use of production factors in 3 different types of sunflower production with respect to orobanche resistance in the agricultural enterprises in Thrace Region which is located at European continent of Turkey. The data used in this research have been obtained through questionnaire technique from 571 agricultural enterprises which were determined by Stratified Random Sampling Method in 2009. It has been reached to the highest yield by 189.30 kg da-1 and the highest gross profit by 37.91 US$ da-1 in the production of sunflower, resistant to orobanche. In the research, it has been determined that the rate of soil testing among the sunflower producers is considerably low and almost the whole of the production has been made under rainfed conditions. As a result of the research, it has been concluded that orobanche resistant sunflower, which has higher water productivity than other cultivars by 367.13 g m/sup 3/, will have a higher proportion in the sunflower cultivation areas in future because of its higher contribution to producer welfare. (author)

  12. Nitrous Oxide Production and Fluxes from Coastal Sediments under Varying Environmental Conditions

    Science.gov (United States)

    Ziebis, W.; Wankel, S. D.; de Beer, D.; Dentinger, J.; Buchwald, C.; Charoenpong, C.

    2014-12-01

    Although coastal zones represent important contributors to the increasing levels of atmospheric nitrous oxide (N2O), it is still unclear which role benthic processes play and whether marine sediments represent sinks or sources for N2O, since interactions among closely associated microbial groups lead to a high degree of variability. In addition, coastal areas are extremely dynamic regions, often exposed to increased nutrient loading and conditions of depleted oxygen. We investigated benthic N2O fluxes and how environmental conditions affect N2O production in different sediments at 2 different geographical locations (German Wadden Sea, a California coastal lagoon). At each location, a total of 32 sediment cores were taken in areas that differed in sediment type, organic content and pore-water nutrient concentrations, as well as in bioturbation activity. Parallel cores were incubated under in-situ conditions, low oxygen and increased nitrate levels for 10 days. Zones of N2O production and consumption were identified in intact cores by N2O microprofiles at the beginning and end of the experiments. In a collaborative effort to determine the dominant sources of N2O, samples were taken throughout the course of the experiments for the determination of the isotopic composition of N2O (as well as nitrate, nitrite and ammonium). Our results indicate that both, nitrate addition and low oxygen conditions in the overlying water, caused an increase of subsurface N2O production in most sediments, with a high variability between different sediment types. N2O production in the sediments was accompanied by N2O consumption, reducing the fluxes to the water column. In general, organic rich sediments showed the strongest response to environmental changes with increased production and efflux of N2O into the overlying water. Bioturbation activity added to the complexity of N2O dynamics by an increase in nitrification-denitrification processes, as well as enhanced pore-water transport

  13. Innovative Equipment and Production Method for Mixed Fodder in the Conditions of Agricultural Enterprises

    Science.gov (United States)

    Sabiev, U. K.; Demchuk, E. V.; Myalo, V. V.; Soyunov, A. S.

    2017-07-01

    It is recommended to feed the cattle and poultry with grain fodder in the form of feed mixture balanced according to the content. Feeding of grain fodder in the form of stock feed is inefficient and economically unreasonable. The article is devoted to actual problem - the preparation of mixed fodder in the conditions of agricultural enterprises. Review and critical analyses of mixed fodder assemblies and aggregates are given. Structural and technical schemes of small-size mixed fodder aggregate with intensified attachments of vibrating and percussive action for preparation of bulk feed mixture in the conditions of agricultural enterprises were developed. The mixed fodder aggregate for its preparation in the places of direct consumption from own grain fodder production and purchased protein and vitamin supplements is also suggested. Mixed fodder aggregate allows to get prepared mixed fodder of high uniformity at low cost of energy and price of production that is becoming profitable for livestock breeding. Model line-up of suggested mixed fodder aggregate with different productivity both for small and big agricultural enterprises is considered.

  14. Disintegration of the agricultural by-product wheat bran under subcritical conditions.

    Science.gov (United States)

    Reisinger, Michael; Tirpanalan, Özge; Pruksasri, Suwattana; Kneifel, Wolfgang; Novalin, Senad

    2018-02-10

    The disintegration of destarched wheat bran in water and sulfuric acid (pH 3) under subcritical conditions (275-300 °C) and at short reaction times (1-4 min) was investigated. A cascade process comprising a stepwise separation of the liquid was applied to reduce the formation of undesired degradation products. The highest degree of biomass disintegration (67% dry mass solubilization) was achieved by application of a cascade process at 275 °C (pH 3). Regarding the dissolution of carbohydrates (monomeric and oligomeric form), the total glucose yields remained below 60%, while the total xylose and arabinose yields were about 76% and 67%. Approximately 74% of the protein and 95% of the mineral fraction could be extracted. The application of the cascade process enabled a substantially reduced formation of degradation products. When operating hydrothermally and subcritically in order to avoid some problematic aspects of a biorefinery, an extensive disintegration and monomerization of wheat bran and its constituents remains difficult even under the tested conditions (300 °C, pH 3). However, the applied cascade process proved to be useful to increase the yields and to substantially reduce the formation of undesired degradation products. Despite this fact, increased water consumption has to be conceded. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry.

  15. Impacts of environmental conditions on product formation and morphology of Yarrowia lipolytica.

    Science.gov (United States)

    Timoumi, Asma; Guillouet, Stéphane E; Molina-Jouve, Carole; Fillaudeau, Luc; Gorret, Nathalie

    2018-05-01

    The yeast Yarrowia lipolytica is an industrially important microorganism with distinctive physiological and metabolic characteristics. A variety of external factors (e.g., pH, temperature, and nutrient availability) influences the behavior of the yeast and may act as stress conditions which the cells must withstand and adapt. In this mini review, the impacts of environmental factors on the morphology and metabolite production by Y. lipolytica are summarized. In this regard, detailed insights into the effectors involved in the dimorphic transition of Y. lipolytica, the cultivation conditions employed, as well as the methods applied for the morphological characterization are highlighted. Concerning the metabolism products, a special focus is addressed on lipid and citric acid metabolites which have attracted significant attention in recent years. The dependence of lipid and citric acid productivity on key process parameters, such as media composition and physico-chemical variables, is thoroughly discussed. This review attempts to provide a recent update on the topic and will serve as a meaningful resource for researchers working in the field.

  16. Relationship between fumonisin production and FUM gene expression in Fusarium verticillioides under different environmental conditions

    DEFF Research Database (Denmark)

    Fanelli, Francesca; Iversen, Anita; Logrieco, Antonio F.

    2013-01-01

    Fusarium verticillioides is the main source of fumonisins, a group of mycotoxins that can contaminate maize-based food and feed and cause diseases in humans and animals. The study of the effect of different environmental conditions on toxin production should provide information that can be used...... to develop strategies to minimize the risk. This study analysed the effect of temperature (15°C-35°C), water activity (aw: 0.999-0.93), salinity (0-125 g l-1 NaCl) and pH (5-8) on the growth and production of fumonisins B1 (FB1), B2 (FB2) and B3 (FB3) and the expression of FUM1 and FUM21 in F....... verticillioides. The highest growth rate was measured at 25°C, aw of 0.998-0.99 and 0-25 g l-1 of NaCl. Optimal conditions for fumonisin production were 30°C, aw of 0.99, 25 g l-1 of NaCl and pH 5; nevertheless, the strain showed a good adaptability and was able to produce moderate levels of fumonisins under...

  17. Optimization of simultaneous saccharification and fermentation conditions with amphipathic lignin derivatives for concentrated bioethanol production.

    Science.gov (United States)

    Cheng, Ningning; Koda, Keiichi; Tamai, Yutaka; Yamamoto, Yoko; Takasuka, Taichi E; Uraki, Yasumitsu

    2017-05-01

    Amphipathic lignin derivatives (A-LDs) prepared from the black liquor of soda pulping of Japanese cedar are strong accelerators for bioethanol production under a fed-batch simultaneous enzymatic saccharification and fermentation (SSF) process. To improve the bioethanol production concentration, conditions such as reaction temperature, stirring program, and A-LDs loadings were optimized in both small scale and large scale fed-batch SSF. The fed-batch SSF in the presence of 3.0g/L A-LDs at 38°C gave the maximum ethanol production and a high enzyme recovery rate. Furthermore, a jar-fermenter equipped with a powerful mechanical stirrer was designed for 1.5L-scale fed-batch SSF to achieve rigorous mixing during high substrate loading. Finally, the 1.5L fed-batch SSF with a substrate loading of 30% (w/v) produced a high ethanol concentration of 87.9g/L in the presence of A-LDs under optimized conditions. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  18. Influence of Different Growth Conditions on the Kefir Grains Production, used in the Kefiran Synthesis

    Directory of Open Access Journals (Sweden)

    Carmen Rodica Pop

    2014-11-01

    Full Text Available The purpose of this study was to optimize the kefir grains biomass production, using milk as culture media. The kefir grains were cultured at different changed conditions (temperature, time, shaker rotating speed, culture media supplemented to evaluate their effects. Results showed that optimal culture conditions were using the organic skim milk, incubated at 25°C for 24 hours with a rotation rate of 125 rpm. According to results, the growth rate was 38.9 g/L for 24 h, at 25°C using the organic milk - OSM, 36.87 g/L during 24 hours, optimal time for propagation process gave 37.93 g/L kefir grains biomass when the effect of temperature level was tested. The homogenization of medium with shaker rotating induced a greater growth rate, it was obtained 38.9 g/L for 24 h, at 25°C using rotation rate at 125 rpm. The growing medium (conventional milk supplemented with different minerals and vitamins may lead to improve the growth conditions of kefir grains biomass. The optimization of the growth environment is very important for achieving the maximum production of kefir grains biomass, substrate necessary to obtain the polysaccharide kefiran

  19. Unfolded protein response is required for Aspergillus oryzae growth under conditions inducing secretory hydrolytic enzyme production.

    Science.gov (United States)

    Tanaka, Mizuki; Shintani, Takahiro; Gomi, Katsuya

    2015-12-01

    Unfolded protein response (UPR) is an intracellular signaling pathway for adaptation to endoplasmic reticulum (ER) stress. In yeast UPR, Ire1 cleaves the unconventional intron of HAC1 mRNA, and the functional Hac1 protein translated from the spliced HAC1 mRNA induces the expression of ER chaperone genes and ER-associated degradation genes for the refolding or degradation of unfolded proteins. In this study, we constructed an ireA (IRE1 ortholog) conditionally expressing strain of Aspergillus oryzae, a filamentous fungus producing a large amount of amylolytic enzymes, and examined the contribution of UPR to ER stress adaptation under physiological conditions. Repression of ireA completely blocked A. oryzae growth under conditions inducing the production of hydrolytic enzymes, such as amylases and proteases. This growth defect was restored by the introduction of unconventional intronless hacA (hacA-i). Furthermore, UPR was observed to be induced by amylolytic gene expression, and the disruption of the transcriptional activator for amylolytic genes resulted in partial growth restoration of the ireA-repressing strain. In addition, a homokaryotic ireA disruption mutant was successfully generated using the strain harboring hacA-i as a parental host. These results indicated that UPR is required for A. oryzae growth to alleviate ER stress induced by excessive production of hydrolytic enzymes. Copyright © 2015 Elsevier Inc. All rights reserved.

  20. Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli

    Directory of Open Access Journals (Sweden)

    Neubauer Peter

    2011-05-01

    Full Text Available Abstract Background The eukaryotic RNase ribonuclease/angiogenin inhibitors (RI are a protein group distinguished by a unique structure - they are composed of hydrophobic leucine-rich repeat motifs (LRR and contain a high amount of reduced cysteine residues. The members of this group are difficult to produce in E. coli and other recombinant hosts due to their high aggregation tendency. Results In this work dithiothreitol (DTT was successfully applied for improving the yield of correctly folded ribonuclease/angiogenin inhibitor in E. coli K12 periplasmic and cytoplasmic compartments. The feasibility of the in vivo folding concepts for cytoplasmic and periplasmic production were demonstrated at batch and fed-batch cultivation modes in shake flasks and at the bioreactor scale. Firstly, the best secretion conditions of RI in the periplasmic space were evaluated by using a high throughput multifactorial screening approach of a vector library, directly with the Enbase fed-batch production mode in 96-well plates. Secondly, the effect of the redox environment was evaluated in isogenic dsbA+ and dsbA- strains at the various cultivation conditions with reducing agents in the cultivation medium. Despite the fusion to the signal peptide, highest activities were found in the cytoplasmic fraction. Thus by removing the signal peptide the positive effect of the reducing agent DTT was clearly proven also for the cytoplasmic compartment. Finally, optimal periplasmic and cytoplasmic RI fed-batch production processes involving externally added DTT were developed in shake flasks and scaled up to the bioreactor scale. Conclusions DTT highly improved both, periplasmic and cytoplasmic accumulation and activity of RI at low synthesis rate, i.e. in constructs harbouring weak recombinant synthesis rate stipulating genetic elements together with cultivation at low temperature. In a stirred bioreactor environment RI folding was strongly improved by repeated pulse addition

  1. Conditions of milk production of family farms located in the municipality of Alegre – ES, Brazil

    Directory of Open Access Journals (Sweden)

    Francisca Carvalho Nascimento Neta

    2015-12-01

    Full Text Available The present study aimed to assess the conditions and practices of production of raw milk used by farmers, as well as the conditions of storage and of the facilities and structures of the locations of the four collective cooling tanks, in the municipality of Alegre – ES. Data on sanitization procedures and physical structure of barns, source and treatment of water, hygiene practices and health status of milkers, udder cleaning and disinfection procedures and sanitary control of the herd was collected. The physical structure of the locations of the tanks was also assessed. The barns were ventilated, protected from moisture and had covers, and few had cemented floor and milking parlor. A low percentage of producers performed pre (21% and post (6% dipping procedures. Only 1/4 of the farmers used the mug test. All equipment and tools were washed after milking, but few farmers (5% used sanitizers and 94% of the farms used untreated water. Only 8% of the milkers had the habit of washing their hands before milking, and 9% of them were aware of IN 62 regulation of the Ministry of Agriculture, Livestock and Supply. None of the facilities had protection against the entrance of insects, rodents and environmental contaminants. They were poorly sized, did not allow the application of adequate sanitary measures and sanitary conditions were deficient. The high level of nonconformities regarding good manufacturing practices for milk production reinforced the need for the implementation of Good Agricultural Practices in rural farms for the purpose of obtaining milk produced according to the quality standards, preventing losses and ensuring the sustainability of dairy production in the region.

  2. Reconstruction of Redox Conditions and Productivity in Coastal Waters of the Bothnian Sea during the Holocene

    Science.gov (United States)

    Dijkstra, N.; Quintana Krupinski, N. B.; Slomp, C. P.

    2014-12-01

    Hypoxia is a growing problem in coastal waters worldwide, and is a well-known cause of benthic mortality. The semi-enclosed Baltic Sea is currently the world's largest human-induced dead zone. During the early Holocene, it experienced several periods of natural hypoxia following the intrusion of seawater into the previous freshwater lake. Recent studies suggest that at that time, the hypoxia expanded north to include the deep basin of the Bothnian Sea. In this study, we assess whether the coastal zone of the Bothnian Sea was also hypoxic during the early Holocene. We analysed a unique sediment record (0 - 30 mbsf) from the Ångermanälven estuary, which was retrieved during the International Ocean Discovery Programme (IODP) Baltic Sea Paleoenvironment Expedition 347 in 2013. Using geochemical proxies and foraminifera abundances, we reconstruct the changes in redox conditions, salinity and productivity in the estuary. Our preliminary results suggest that bottom waters in this coastal basin became anoxic upon the intrusion of brackish seawater in the early Holocene and that the productivity was elevated. The presence of benthic foraminifera in this estuary during the mid-Holocene suggests more saline conditions in the Bothnian Sea than today. Due to isostatic uplift, the estuary likely gradually became more isolated from the Bothnian Sea, which itself became more isolated from the Baltic Sea. Both factors likely explain the subsequent re-oxygenation of bottom waters and gradual refreshening of the estuary as recorded in the sediments. Interestingly, the upper meters of sediment are enriched in minerals that contain iron, phosphorus and manganese. We postulate that the refreshening of the estuary triggered the formation of these minerals, thereby increasing the phosphorus retention in these sediments and further reducing primary productivity. This enhanced retention linked to refreshening may contribute to the current oligotrophic conditions in the Bothnian Sea.

  3. Influence of Pyrolysis Temperature and Production Conditions on Switchgrass Biochar for Use as a Soil Amendment

    Directory of Open Access Journals (Sweden)

    Amanda Joy Ashworth

    2014-10-01

    Full Text Available Biochars form recalcitrant carbon and increase water and nutrient retention in soils; however, the magnitude is contingent upon production conditions and thermo-chemical conversion processes. Herein we aim at (i characterizing switchgrass (Panicum virgatum L.-biochar morphology, (ii estimating water-holding capacity under increasing ratios of char: soil; and, (iii determining nutrient profile variation as a function of pyrolysis conversion methodologies (i.e. continuous, auger pyrolysis system versus batch pyrolysis systems for terminal use as a soil amendment. Auger system chars produced at 600°C had the greatest lignin portion by weight among the biochars produced from the continuous system. On the other hand, a batch pyrolysis system (400 °C – 3h yielded biochar with 73.10% lignin (12 fold increases, indicating higher recalcitrance, whereas lower production temperatures (400 °C yielded greater hemicellulose (i.e. greater mineralization promoting substrate. Under both pyrolysis methods, increasing biochar soil application rates resulted in linear decreases in bulk density (g cm-3. Increases in auger-char (400 °C applications increased soil water-holding capacities; however, application rates of >2 Mt ha-1 are required. Pyrolysis batch chars did not influence water-holding abilities (P>0.05. Biochar macro and micronutrients increased, as the pyrolysis temperature increased in the auger system from 400 to 600 °C, and the residence time increased in the batch pyrolysis system from 1 to 3 h. Conversely, nitrogen levels tended to decrease under the two previously mentioned conditions. Consequently, not all chars are inherently equal, in that varying operation systems, residence times, and production conditions greatly affect uses as a soil amendment and overall rate of efficacy.

  4. Production of aflatexin B1 in wheat grains under different environmental storage conditions

    International Nuclear Information System (INIS)

    Mahrous, S.R.; Shahin, A.A.M.; Bothaina, M.Y.

    2000-01-01

    Fungal flora of stored wheat grains and the production of aflatoxin B 1 by Aflavus in wheat grains under different environmental conditions were examined. Aspergillus, Penicillium,. Fusarium, Cladosporium, Curvularia, Epicouccum, Verticilium, Rhizopus, Mucor and Altenaria were the predominant fungi isolated from the collected non-disinfected grains. Aspergillus spp, were only isolated from surface disinfected grains. Of 223 aspergillus spp, isolates only 128 found to aflatoxin producing and all aflatoxin producing-fungi belonged to the Aflavus group. Results demonstrate that Aflavus could produce maximum concentration of aflatoxin B 1 in grains at 20% moisture (163.5 MOU g/kg). The highest concentration of aflatoxin B 1 was produced by Aflavus (10 5 spores/g) in wheat grains with 20% moisture after 20 days at 30 degree and 92.40 % R.H. The aflatoxin production did not increase monotonously as a function of inoculum density

  5. Biochemical basis of drought tolerance in hybrid Populus grown under field production conditions. CRADA final report

    Energy Technology Data Exchange (ETDEWEB)

    Tschaplinski, T.J.; Tuskan, G.A. [Oak Ridge National Lab., TN (United States); Wierman, C. [Boise Cascade Corp., Wallula, WA (United States)

    1997-04-01

    The purpose of this cooperative effort was to assess the use of osmotically active compounds as molecular selection criteria for drought tolerance in Populus in a large-scale field trial. It is known that some plant species, and individuals within a plant species, can tolerate increasing stress associated with reduced moisture availability by accumulating solutes. The biochemical matrix of such metabolites varies among species and among individuals. The ability of Populus clones to tolerate drought has equal value to other fiber producers, i.e., the wood products industry, where irrigation is used in combination with other cultural treatments to obtain high dry weight yields. The research initially involved an assessment of drought stress under field conditions and characterization of changes in osmotic constitution among the seven clones across the six moisture levels. The near-term goal was to provide a mechanistic basis for clonal differences in productivity under various irrigation treatments over time.

  6. The Behavior of Corrosion Products in Sampling Systems under Boiling Water Reactor Conditions

    Energy Technology Data Exchange (ETDEWEB)

    Hermansson, Hans-Peter

    1977-08-15

    A high pressure loop has been used to simulate sampling systems employed under BWR conditions. The reliability of the sampling method was studied in a series of six test runs. A variety of parameters that are thought to influence the reliability of the sampling was investigated. These included piping geometry, water oxygen content, flow, temperature and temperature gradients. Amongst other things the results indicate that the loss by deposition of iron containing corrosion products does not exceed 50 %; this figure is only influenced to a minor extent by the above mentioned parameters. The major part of the corrosion products thus deposited is found along the first few meters of the piping and cooler coil. A moderate prolongation of a pipe which is already relatively long should thus be incapable of producing a major influence on the sampling error

  7. Dissolution Coupled Biodegradation of Pce by Inducing In-Situ Biosurfactant Production Under Anaerobic Conditions

    Science.gov (United States)

    Dominic, J.; Nambi, I. M.

    2013-12-01

    Biosurfactants have proven to enhance the bioavailability and thereby elevate the rate of degradation of Light Non Aqueous Phase Liquids (LNAPLs) such as crude oil and petroleum derivatives. In spite of their superior characteristics, use of these biomolecules for remediation of Dense Non Aqueous Phase Liquids (DNAPLs) such as chlorinated solvents is still not clearly understood. In this present study, we have investigated the fate of tetrachloroethylene (PCE) by inducing in-situ biosurfactants production, a sustainable option which hypothesizes increase in bioavailability of LNAPLs. In order to understand the effect of biosurfactants on dissolution and biodegradation under the inducement of in-situ biosurfactant production, batch experiments were conducted in pure liquid media. The individual influence of each process such as biosurfactant production, dissolution of PCE and biodegradation of PCE were studied separately for getting insights on the synergistic effect of each process on the fate of PCE. Finally the dissolution coupled biodegradation of non aqueous phase PCE was studied in conditions where biosurfactant production was induced by nitrate limitation. The effect of biosurfactants was differentiated by repeating the same experiments were the biosurfactant production was retarded. The overall effect of in-situ biosurfactant production process was evaluated by use of a mathematical model. The process of microbial growth, biosurfactant production, dissolution and biodegradation of PCE were translated as ordinary differential equations. The modelling exercise was mainly performed to get insight on the combined effects of various processes that determine the concentration of PCE in its aqueous and non-aqueous phases. Model simulated profiles of PCE with the kinetic coefficients evaluated earlier from individual experiments were compared with parameters fitted for observations in experiments with dissolution coupled biodegradation process using optimization

  8. Poly(3-hydroxybutyrate production in an integrated electromicrobial setup: Investigation under stress-inducing conditions.

    Directory of Open Access Journals (Sweden)

    Israa Salem Al Rowaihi

    Full Text Available Poly(3-hydroxybutyrate (PHB, a biodegradable polymer, can be produced by different microorganisms. The PHB belongs to the family of polyhydroxyalkanoate (PHA that mostly accumulates as a granule in the cytoplasm of microorganisms to store carbon and energy. In this study, we established an integrated one-pot electromicrobial setup in which carbon dioxide is reduced to formate electrochemically, followed by sequential microbial conversion into PHB, using the two model strains, Methylobacterium extorquens AM1 and Cupriavidus necator H16. This setup allows to investigate the influence of different stress conditions, such as coexisting electrolysis, relatively high salinity, nutrient limitation, and starvation, on the production of PHB. The overall PHB production efficiency was analyzed in reasonably short reaction cycles typically as short as 8 h. As a result, the PHB formation was detected with C. necator H16 as a biocatalyst only when the electrolysis was operated in the same solution. The specificity of the source of PHB production is discussed, such as salinity, electricity, concurrent hydrogen production, and the possible involvement of reactive oxygen species (ROS.

  9. Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions

    Science.gov (United States)

    Al Rowaihi, Israa Salem; Paillier, Alexis; Rasul, Shahid; Karan, Ram; Grötzinger, Stefan Wolfgang; Eppinger, Jörg

    2018-01-01

    Poly(3-hydroxybutyrate) (PHB), a biodegradable polymer, can be produced by different microorganisms. The PHB belongs to the family of polyhydroxyalkanoate (PHA) that mostly accumulates as a granule in the cytoplasm of microorganisms to store carbon and energy. In this study, we established an integrated one-pot electromicrobial setup in which carbon dioxide is reduced to formate electrochemically, followed by sequential microbial conversion into PHB, using the two model strains, Methylobacterium extorquens AM1 and Cupriavidus necator H16. This setup allows to investigate the influence of different stress conditions, such as coexisting electrolysis, relatively high salinity, nutrient limitation, and starvation, on the production of PHB. The overall PHB production efficiency was analyzed in reasonably short reaction cycles typically as short as 8 h. As a result, the PHB formation was detected with C. necator H16 as a biocatalyst only when the electrolysis was operated in the same solution. The specificity of the source of PHB production is discussed, such as salinity, electricity, concurrent hydrogen production, and the possible involvement of reactive oxygen species (ROS). PMID:29698424

  10. Optimization of cultural and nutritional conditions for carboxymethylcellulase production by Aspergillus hortai

    Directory of Open Access Journals (Sweden)

    Abeer A. El-Hadi

    2014-01-01

    Full Text Available The potential production of carboxymethylcellulase (CMCase by Aspergillus hortai in liquid state fermentation was studied. Cultural and nutritional factors affecting CMC production were also investigated in order to optimize the fermentation conditions for the maximization of production. The obtained results revealed that, the maximum CMCase production (0.23 U/ml was achieved after 96 h in a liquid medium (PH7 inoculated with 10% v/v, at temperature 37 °C, containing (g L−1 CMC, 5.0; yeast extract, 0.1; (NH4SO4, 0.5; KH2PO4, 10.0; MgSO4·7H2O, 0.1 and NaCl, 0.2. and the activity remained almost stable between pH 6 and 7. The highest CMCase activity (1.18 U/ml was obtained at a lactose concentration of 5.0 g L-1.

  11. A Review on the Assessment of Stress conditions for Simultaneous Production of Microalgal Lipids and Carotenoids

    Directory of Open Access Journals (Sweden)

    Amritpreet kaur Minhas

    2016-05-01

    Full Text Available Microalgal species are potential resource of both biofuels and high-value metabolites, and their production is growth dependent. Growth parameters can be screened for the selection of novel microalgal species that produce molecules of interest. In this context our review confirms that, autotrophic and heterotrophic organisms have demonstrated a dual potential, namely the ability to produce lipids as well as value-added products (particularly carotenoids under influence of various physico-chemical stresses on microalgae. Some species of microalgae can synthesize, besides some pigments, very-long-chain polyunsaturated fatty acids (VL-PUFA,>20C such as docosahexaenoic acid and eicosapentaenoic acid, those have significant applications in food and health. Producing value-added by-products in addition to biofuels, fatty acid methyl esters (FAME, and lipids has the potential to improve microalgae-based biorefineries by employing either the autotrophic or the heterotrophic mode, which could be an offshoot of biotechnology. The review considers the potential of microalgae to produce a range of products and indicates future directions for developing suitable criteria for choosing novel isolates through bioprospecting large gene pool of microalga obtained from various habitats and climatic conditions.

  12. A Review on the Assessment of Stress Conditions for Simultaneous Production of Microalgal Lipids and Carotenoids

    Science.gov (United States)

    Minhas, Amritpreet K.; Hodgson, Peter; Barrow, Colin J.; Adholeya, Alok

    2016-01-01

    Microalgal species are potential resource of both biofuels and high-value metabolites, and their production is growth dependent. Growth parameters can be screened for the selection of novel microalgal species that produce molecules of interest. In this context our review confirms that, autotrophic and heterotrophic organisms have demonstrated a dual potential, namely the ability to produce lipids as well as value-added products (particularly carotenoids) under influence of various physico-chemical stresses on microalgae. Some species of microalgae can synthesize, besides some pigments, very-long-chain polyunsaturated fatty acids (VL-PUFA,>20C) such as docosahexaenoic acid and eicosapentaenoic acid, those have significant applications in food and health. Producing value-added by-products in addition to biofuels, fatty acid methyl esters (FAME), and lipids has the potential to improve microalgae-based biorefineries by employing either the autotrophic or the heterotrophic mode, which could be an offshoot of biotechnology. The review considers the potential of microalgae to produce a range of products and indicates future directions for developing suitable criteria for choosing novel isolates through bioprospecting large gene pool of microalga obtained from various habitats and climatic conditions. PMID:27199903

  13. Under which climate and soil conditions the plant productivity-precipitation relationship is linear or nonlinear?

    Science.gov (United States)

    Ye, Jian-Sheng; Pei, Jiu-Ying; Fang, Chao

    2018-03-01

    Understanding under which climate and soil conditions the plant productivity-precipitation relationship is linear or nonlinear is useful for accurately predicting the response of ecosystem function to global environmental change. Using long-term (2000-2016) net primary productivity (NPP)-precipitation datasets derived from satellite observations, we identify >5600pixels in the North Hemisphere landmass that fit either linear or nonlinear temporal NPP-precipitation relationships. Differences in climate (precipitation, radiation, ratio of actual to potential evapotranspiration, temperature) and soil factors (nitrogen, phosphorous, organic carbon, field capacity) between the linear and nonlinear types are evaluated. Our analysis shows that both linear and nonlinear types exhibit similar interannual precipitation variabilities and occurrences of extreme precipitation. Permutational multivariate analysis of variance suggests that linear and nonlinear types differ significantly regarding to radiation, ratio of actual to potential evapotranspiration, and soil factors. The nonlinear type possesses lower radiation and/or less soil nutrients than the linear type, thereby suggesting that nonlinear type features higher degree of limitation from resources other than precipitation. This study suggests several factors limiting the responses of plant productivity to changes in precipitation, thus causing nonlinear NPP-precipitation pattern. Precipitation manipulation and modeling experiments should combine with changes in other climate and soil factors to better predict the response of plant productivity under future climate. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Genotypic interactions with potassium nutrition on fruit production in cotton (gossypium hirsutum l.) under irrigated conditions

    International Nuclear Information System (INIS)

    Makhdum, M.I.; Pervez, H.; Ashraf, M.

    2005-01-01

    A field experiment was conducted using four (Gossypium hirsutum l.) cultivars (OM-448, OM-I00, NIAB-Karishma, 5-12) at four rates of potassium (0, 62, 5, 125, 250 kg K ha-1) and with two sources of potassium (K/sub 2/S0/sub 4/, KCI) to determine the effects of potassium (K) fertilizer on fruit production under irrigated conditions. Cultivars differed significantly amongst themselves in production and retention of fruits per unit land area. The cultivars were categorized as OM-448>OM-1100>Karishma>5-12 in order of fruit production. The number of total fruiting positions increased with concurrent levels of K-fertilizer. The shedding of fruit was significantly reduced by application of 250 kg K ha-1 compared to zero K-rate treatment. The addition of K-fertilizer in the form of K/sub 2/S0/sub 4/ showed an edge over KCI in fruit production. A high degree of correlation (r 0.89**,0.91**, -0.8**) was measured between seed cotton yield and number of total fruiting positions, number of intact fruit and fruit shedding percentage respectively. (author)

  15. Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions

    KAUST Repository

    Al Rowaihi, Israa Salem

    2018-04-26

    Poly(3-hydroxybutyrate) (PHB), a biodegradable polymer, can be produced by different microorganisms. The PHB belongs to the family of polyhydroxyalkanoate (PHA) that mostly accumulates as a granule in the cytoplasm of microorganisms to store carbon and energy. In this study, we established an integrated one-pot electromicrobial setup in which carbon dioxide is reduced to formate electrochemically, followed by sequential microbial conversion into PHB, using the two model strains, Methylobacterium extorquens AM1 and Cupriavidus necator H16. This setup allows to investigate the influence of different stress conditions, such as coexisting electrolysis, relatively high salinity, nutrient limitation, and starvation, on the production of PHB. The overall PHB production efficiency was analyzed in reasonably short reaction cycles typically as short as 8 h. As a result, the PHB formation was detected with C. necator H16 as a biocatalyst only when the electrolysis was operated in the same solution. The specificity of the source of PHB production is discussed, such as salinity, electricity, concurrent hydrogen production, and the possible involvement of reactive oxygen species (ROS).

  16. Monitoring of conditions inside gas aggregation cluster source during production of Ti/TiOx nanoparticles

    Science.gov (United States)

    Kousal, J.; Kolpaková, A.; Shelemin, A.; Kudrna, P.; Tichý, M.; Kylián, O.; Hanuš, J.; Choukourov, A.; Biederman, H.

    2017-10-01

    Gas aggregation sources are nowadays rather widely used in the research community for producing nanoparticles. However, the direct diagnostics of conditions inside the source are relatively scarce. In this work, we focused on monitoring the plasma parameters and the composition of the gas during the production of the TiOx nanoparticles. We studied the role of oxygen in the aggregation process and the influence of the presence of the particles on the plasma. The construction of the source allowed us to make a 2D map of the plasma parameters inside the source.

  17. Air-conditioning in the 21st century: impact on human productivity and energy consumption

    DEFF Research Database (Denmark)

    Fanger, Povl Ole

    2000-01-01

    Although air-conditioning has played a positive role for economic development in warm climates, its image is globally mixed. Field studies demonstrate that there are substantial numbers of dissatisfied people in many buildings, among them those suffering from Sick Building Syndrome (SBS) symptoms...... environment on human productivity, health and comfort. The principles of excellence can be provided with moderate energy consumption. But the success of excellent indoor environments will increase the demand for improvement globally and the required energy supply will provide a challenge for the world...

  18. Effect of mode of operation on hydrogen production from glycerol at thermal neutral conditions: Thermodynamic analysis

    Energy Technology Data Exchange (ETDEWEB)

    Pairojpiriyakul, Thirasak; Soottitantawat, Apinan; Arpornwichanop, Amornchai; Assabumrungrat, Suttichai [Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University (Thailand); Kiatkittipong, Worapon [Department of Chemical Engineering, Faculty of Engineering and Industrial Technology, Silpakorn University (Thailand); Wiyaratn, Wisitsree [Department of Production Technology Education, Faculty of Industrial Education and Technology, King Mongkut' s University of Technology Thonburi (Thailand); Laosiripojana, Navadol [The Joint Graduate School of Energy and Environment, King Mongkut' s University of Technology Thonburi (Thailand); Croiset, Eric [Department of Chemical Engineering, University of Waterloo (Canada)

    2010-10-15

    Thermodynamic analysis of hydrogen production from glycerol under thermal neutral conditions is studied in this work. Heat requirement from the process can be achieved from the exothermic reaction of glycerol with oxygen in air fed to the system. Two modes of operation for air feeding are considered including (i) Single-feed mode in which air is fed in combination with water and glycerol to the reformer, and (ii) Split-feed mode in which air and part of glycerol is fed to a combustor in order to generate heat. The thermal neutral conditions are considered for two levels including Reformer and System levels. It was found that the H{sub 2} yield from both modes is not significantly different at the Reformer level. In contrast, the difference becomes more pronounced at the System level. Single-feed and Split-feed modes offer high H{sub 2} yield in low (600-900 K) and high (900-1200 K) temperature ranges, respectively. The maximum H{sub 2} yields are 5.67 (water to glycerol ratio, WGR = 12, oxygen to glycerol ratio, OGR = 0.37, T = 900 K, Split-feed mode), and 3.28 (WGR = 3, OGR = 1.40, T = 900 K, Single-feed mode), for the Reformer and System levels, respectively. The difference between H{sub 2} yields in both levels mainly arises from the huge heat demand for preheating feeds in the System level, and therefore, a higher amount of air is needed to achieve the thermal neutral condition. Split-feed mode is a favorable choice in term of H{sub 2} purity because the gas product is not diluted with N{sub 2} from the air. The use of pure O{sub 2} and afterburner products (ABP) stream were also considered at the System level. The maximum H{sub 2} yield becomes 3.75 (WGR = 5.21, OGR = 1.28, T = 900 K, Split-feed mode) at thermal neutral condition when utilizing heat from the ABP stream. Finally comparisons between the different modes and levels are addressed in terms of yield of by-products, and carbon formation. (author)

  19. Homogeneity and heterogeneity in amylase production by Bacillus subtilis under different growth conditions.

    Science.gov (United States)

    Ploss, Tina N; Reilman, Ewoud; Monteferrante, Carmine G; Denham, Emma L; Piersma, Sjouke; Lingner, Anja; Vehmaanperä, Jari; Lorenz, Patrick; van Dijl, Jan Maarten

    2016-03-29

    Bacillus subtilis is an important cell factory for the biotechnological industry due to its ability to secrete commercially relevant proteins in large amounts directly into the growth medium. However, hyper-secretion of proteins, such as α-amylases, leads to induction of the secretion stress-responsive CssR-CssS regulatory system, resulting in up-regulation of the HtrA and HtrB proteases. These proteases degrade misfolded proteins secreted via the Sec pathway, resulting in a loss of product. The aim of this study was to investigate the secretion stress response in B. subtilis 168 cells overproducing the industrially relevant α-amylase AmyM from Geobacillus stearothermophilus, which was expressed from the strong promoter P(amyQ)-M. Here we show that activity of the htrB promoter as induced by overproduction of AmyM was "noisy", which is indicative for heterogeneous activation of the secretion stress pathway. Plasmids were constructed to allow real-time analysis of P(amyQ)-M promoter activity and AmyM production by, respectively, transcriptional and out-of-frame translationally coupled fusions with gfpmut3. Our results show the emergence of distinct sub-populations of high- and low-level AmyM-producing cells, reflecting heterogeneity in the activity of P(amyQ)-M. This most likely explains the heterogeneous secretion stress response. Importantly, more homogenous cell populations with regard to P(amyQ)-M activity were observed for the B. subtilis mutant strain 168degUhy32, and the wild-type strain 168 under optimized growth conditions. Expression heterogeneity of secretory proteins in B. subtilis can be suppressed by degU mutation and optimized growth conditions. Further, the out-of-frame translational fusion of a gene for a secreted target protein and gfp represents a versatile tool for real-time monitoring of protein production and opens novel avenues for Bacillus production strain improvement.

  20. Chitosan production by psychrotolerant Rhizopus oryzae in non-sterile open fermentation conditions.

    Science.gov (United States)

    Tasar, Ozden Canli; Erdal, Serkan; Taskin, Mesut

    2016-08-01

    A new chitosan producing fungus was locally isolated from soil samples collected around Erzurum, Turkey and identified as Rhizopus oryzae PAS 17 (GenBank accession number KU318422.1). Cultivation in low cost non-sterile conditions was achieved by exploiting its ability to grow at low temperature and pH, thus, undesired microbial contamination could be eliminated when appropriate culture conditions (incubation temperature as 15°C and initial pH of the medium as 4.5) were selected. Medium composition and culture conditions were optimized using Taguchi orthogonal array (OA) design of experiment (DOE). An OA layout of L16 (4(5)) was constructed with five most influensive factors at four levels on chitosan production like, carbon source (molasses), metal ion (Mg(2+)), inoculum amount, agitation speed and incubation time. The optimal combinations of factors (molasses, 70ml/l; MgSO4·7H2O, 0.5g/l; inoculum, 6.7×10(6) spores/disc; agitation speed, 150rpm and incubation time, 8days) obtained from the proposed DOE methodology was further validated by analysis of variance (ANOVA) test and the results revealed the increment of chitosan and biomass yields of 14.45 and 8.58 folds from its unoptimized condition, respectively. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. Simulated conditions of microgravity suppress progesterone production by luteal cells of the pregnant rat

    Science.gov (United States)

    Bhat, G. K.; Yang, H.; Sridaran, R.

    2001-01-01

    The purpose of this study was to assess whether simulated conditions of microgravity induce changes in the production of progesterone by luteal cells of the pregnant rat ovary using an in vitro model system. The microgravity environment was simulated using either a high aspect ratio vessel (HARV) bioreactor with free fall or a clinostat without free fall of cells. A mixed population of luteal cells isolated from the corpora lutea of day 8 pregnant rats was attached to cytodex microcarrier beads (cytodex 3). These anchorage dependent cells were placed in equal numbers in the HARV or a spinner flask control vessel in culture conditions. It was found that HARV significantly reduced the daily production of progesterone from day 1 through day 8 compared to controls. Scanning electron microscopy showed that cells attached to the microcarrier beads throughout the duration of the experiment in both types of culture vessels. Cells cultured in chamber slide flasks and placed in a clinostat yielded similar results when compared to those in the HARV. Also, when they were stained by Oil Red-O for lipid droplets, the clinostat flasks showed a larger number of stained cells compared to control flasks at 48 h. Further, the relative amount of Oil Red-O staining per milligram of protein was found to be higher in the clinostat than in the control cells at 48 h. It is speculated that the increase in the level of lipid content in cells subjected to simulated conditions of microgravity may be due to a disruption in cholesterol transport and/or lesions in the steroidogenic pathway leading to a fall in the synthesis of progesterone. Additionally, the fall in progesterone in simulated conditions of microgravity could be due to apoptosis of luteal cells.

  2. Analysis of corrosion product transport in PWR primary system under non-convective condition

    International Nuclear Information System (INIS)

    Han, Byoung Sub

    1992-02-01

    The increase of occupational radiation exposure (ORE) due to the increase of the operational period at existing nuclear power plant and also the publication of the new version of ICRP recommendation (ICRP publication No. 60) for radiological protection require much more strict reduction of radiation buildup in the nuclear power plant. The major sources of the radiation, i.e. the radioactive corrosion-products, are generated by the neutron activation of the corrosion products at the reactor core, and then the radioactive corrosion products are transported to the outside of the core, and accumulated near the steam generator side at PWR. Major radioactive corrosion-products of interest in PWR are Cr 51 ,: Mn 54 ,: Co 58 ,: Fe 59 and Co 60 . Among them Co 58 and Co 60 are known to contribute approximately more than 70% of the total ORE. Thus our main concerns are focused on predicting the transport and deposition of the Co radionuclides and suggesting the optimizing method which can minimize and control the ORE of the nuclear power plant. It is well known that Co-source is most effectively controlled by pH-solubility radiation control, and also some complex computer codes such as CORA and PACTOLE have been developed and revised to predict the corrosion product behavior. However these codes still imply some intrisic problems in simulating the real behavior of corrosion products in the reactor because of 1) the lack of important experimental data, coefficients and parameters of the transport and reactions under actual high temperature and pressure conditions, 2) no general theoretical modelling which can describe such many different mechanisms involved in the corrosion product movements, 3) the newly developed and measured behavior of the corrosion product transport mechanism. Since no sufficient and detailed information is available from the above-mentioned codes (also due to propriority problems), we concentrate on developing a new computer code, CP-TRAN (Corrosion

  3. Productivity of Sumateran Composite dan Barbados Cross sheep breed in the field condition

    Directory of Open Access Journals (Sweden)

    Bambang Setiadi

    2007-12-01

    Full Text Available Two years field study has been carried out in the Purwakarta district , West Java, to evaluate sheep productivity of Sumateran Composite (K and Barbados cross (BC breeds introduced to the farmers, compared with locally thin tail sheep breed (L that were maintained by the farmers. Genotype compositions of K sheep were Barbados Blackbelly 25%, St. Croix 25%, and Sumateran sheep 50%; and for BC were Barbados Blackbelly 50% and Sumateran sheep 50%. Sheep those were introduced was new breed from breeding improvement of Indonesian Research Institute for Animal Production. Litter size of K, BC, and L ewes was 1.3; 1.4; and 1.5 respectively. Pre-weaning mortality rate were 5.0; 5.0 and 8.0% respectively for K, BC, and L lambs. Ewe Reproduction Rate (LRI = number of lamb at weaning/ewe/year of L (2.14 was higher than BC (2.0 and K (1.85. The ewe productivity (PI = kg lambs/ewe/year is the average of weaning weight timed LRI. Because the weaning weight of L (7.0 kg were significantly lowest than BC (10.5 kg and K (9.25 kg will affect on PI. PI of L (14.98 ewe were significantly (P<00.5 lowest than BC (21 and K (17.11. Body weight of crossbred (K X L and BCxL under the same physiologic status were similarly with BC and K. According to the result of productivity evaluation of introduced sheep breed in the field condition, can be sumarize that productivity of K and BC sheep were significantly better than locally thin tail sheep.

  4. Continuous fermentative hydrogen production from cheese whey wastewater under thermophilic anaerobic conditions

    Energy Technology Data Exchange (ETDEWEB)

    Azbar, Nuri; Cetinkaya Dokgoez, F. Tuba; Keskin, Tugba; Korkmaz, Kemal S.; Syed, Hamid M. [Bioengineering Department, Faculty of Engineering, Ege University, EBILTEM, Bornova, 35100 Izmir (Turkey)

    2009-09-15

    Hydrogen (H{sub 2}) production from cheese processing wastewater via dark anaerobic fermentation was conducted using mixed microbial communities under thermophilic conditions. The effects of varying hydraulic retention time (HRT: 1, 2 and 3.5 days) and especially high organic load rates (OLR: 21, 35 and 47 g chemical oxygen demand (COD)/l/day) on biohydrogen production in a continuous stirred tank reactor were investigated. The biogas contained 5-82% (45% on average) hydrogen and the hydrogen production rate ranged from 0.3 to 7.9 l H{sub 2}/l/day (2.5 l/l/day on average). H{sub 2} yields of 22, 15 and 5 mmol/g COD (at a constant influent COD of 40 g/l) were achieved at HRT values of 3.5, 2, and 1 days, respectively. On the other hand, H{sub 2} yields were monitored to be 3, 9 and 6 mmol/g COD, for OLR values of 47, 35 and 21 g COD/l/day, when HRT was kept constant at 1 day. The total measurable volatile fatty acid concentration in the effluent (as a function of influent COD) ranged between 118 and 27,012 mg/l, which was mainly composed of acetic acid, iso-butyric acid, butyric acid, propionic acid, formate and lactate. Ethanol and acetone production was also monitored from time to time. To characterize the microbial community in the bioreactor at different HRTs, DNA in mixed liquor samples was extracted immediately for PCR amplification of 16S RNA gene using eubacterial primers corresponding to 8F and 518R. The PCR product was cloned and subjected to DNA sequencing. The sequencing results were analyzed by using MegaBlast available on NCBI website which showed 99% identity to uncultured Thermoanaerobacteriaceae bacterium. (author)

  5. Production and processing of fuel by the forest industry - opportunities and conditions

    International Nuclear Information System (INIS)

    Magnusson, L.

    1991-01-01

    The purpose of this study was to illustrate the opportunities for the forest industry to establish a system of handling and processing biofuels in conjunction with their existing activities, and which would supply a future market for biofuels in, for example, electricity generation. The sawmills report that it is difficult today to find a market for fuel products, especially for sawmills at greater distances from larger biofuel-consuming plants. The sawmills show great interest in cogeneration in their own plants, but report difficulties in achieving profitability. The main problem is reported to be that the price of the surplus electricity delivered to the grid is too low, but also that the electricity prices today are so low that it is difficult to justify even generating electricity for the mill's own use. There is an interest in the paper and pulp industry for integrated methods and production of biofuels since the part-tree methods used, at least in some parts of Sweden, are considered to contribute also to an increase in the availability of pulp wood to the industry. A fundamental viewpoint is, however, that the plants are built for the primary purpose of producing pulp or paper. It is unlikely that the industry would give priority to investments for production of a new secondary product in the form of fuel products, particularly when the conditions today imply that there are few possibilities to achieve any particular profitability. The most probable solution is that the fuel is processed outside the industry by other parties, e.g., the forest divisions. In the long term, increased efficiency in the processes may lead to a primary heat surplus which could be used to produce processed fuels

  6. Thermal Transport and Entropy Production Mechanisms in a Turbulent Round Jet at Supercritical Thermodynamic Conditions

    Directory of Open Access Journals (Sweden)

    Florian Ries

    2017-08-01

    Full Text Available In the present paper, thermal transport and entropy production mechanisms in a turbulent round jet of compressed nitrogen at supercritical thermodynamic conditions are investigated using a direct numerical simulation. First, thermal transport and its contribution to the mixture formation along with the anisotropy of heat fluxes and temperature scales are examined. Secondly, the entropy production rates during thermofluid processes evolving in the supercritical flow are investigated in order to identify the causes of irreversibilities and to display advantageous locations of handling along with the process regimes favorable to mixing. Thereby, it turned out that (1 the jet disintegration process consists of four main stages under supercritical conditions (potential core, separation, pseudo-boiling, turbulent mixing, (2 causes of irreversibilities are primarily due to heat transport and thermodynamic effects rather than turbulence dynamics and (3 heat fluxes and temperature scales appear anisotropic even at the smallest scales, which implies that anisotropic thermal diffusivity models might be appropriate in the context of both Reynolds-averaged Navier–Stokes (RANS and large eddy simulation (LES approaches while numerically modeling supercritical fluid flows.

  7. Rapid production of Candida albicans chlamydospores in liquid media under various incubation conditions.

    Science.gov (United States)

    Alicia, Zavalza-Stiker; Blanca, Ortiz-Saldivar; Mariana, García-Hernández; Magdalena, Castillo-Casanova; Alexandro, Bonifaz

    2006-01-01

    The production of chlamydospores is a diagnostic tool used to identify Candida albicans; these structures also represent a model for morphogenetic research. The time required to produce them with standard methods is 48-72 hours in rice meal agar and tensoactive agents. This time can be shorted using liquid media such as cornmeal broth (CMB) and dairy supplements. Five media were tested: CMB plus 1% Tween-80, CMB plus 5% milk, CMB plus 5% milk serum, milk serum, and milk serum plus 1% Tween-80, under different incubation conditions: at 28 degrees C and 37 degrees C in a metabolic bath stirring at 150 rpm, and at 28 degrees C in a culture stove. The reading time points were established at 8 and 16 hours. The best results were obtained at 16 hours with CMB plus 5% milk under incubation at 28 degrees C and stirring at 150 rpm. The next most efficient methods were CMB plus 5% milk serum and CMB plus 1% Tween-80, under the same incubation conditions. The other media were ineffective in producing chlamydospores. The absence of stirring at 28 degrees C prevented the formation of chlamydospores within the set time points, and incubation at 37 degrees C decreased their production. This paper reports that the time to form C. albicans chlamydospores can be reduced.

  8. Hydrogen production by Chlamydomonas reinhardtii under light driven sulfur deprived condition

    Energy Technology Data Exchange (ETDEWEB)

    Vijayaraghavan, Krishnan; Karthik, Rajendran [Biotechnology Research Division, Department of Biotechnology, Prathyusha Institute of Technology and Management, Aranvoyalkuppam, Thiruvallur District 602025, Tamil Nadu (India); Kamala Nalini, S.P. [Department of Biotechnology, Vel Group of Educational Institutions, Avadi, Alamadhi Road, Chennai 600062, Tamil Nadu (India)

    2009-10-15

    This article explores the possibility of demonstrating sustainable photohydrogen production using Chlamydomonas reinhardtii when grown in sulfur deprived photoautotrophic condition. The hydrogen evolving capability of the algal species was monitored based on alternating light and dark period. Investigation was carried out during the day time in order to exploit the solar energy for meeting the demand of the light period. The results showed that when the reactor was operated at varying photoperiod namely 2, 3 and 4 h of alternating light and dark period, the gas generation was found to be 32 {+-} 4, 63 {+-} 7 and 52 {+-} 5 mL/h, while the corresponding hydrogen content was 47, 86 and 87% respectively. Functional components of hydrogen generation reaction centers were also analyzed, which showed that the PS(I) reaction centers were involved in hydrogen production pathway, as the light absorption by PS(I) was prerequisite for hydrogen generation under sulfur deprived photoautotrophic condition. The findings showed a higher gas yield and hydrogen content under dark period, whereas under light period the gas content was below detectable level for hydrogen due to the reversible hydrogenase reaction. (author)

  9. Egg quality in layers housed in different production systems and submitted to two environmental conditions

    Directory of Open Access Journals (Sweden)

    JAD Barbosa Filho

    2006-03-01

    Full Text Available The production system using cages is a highly polemical issue in Europe, because of the space restriction imposed to laying hens. It is considered that the cage system might compromise important comfort movements, welfare and egg quality. This study evaluated egg quality and welfare of two strains of hens housed in a conventional system (battery laying cages or litter system with nest and perches, and submitted to heat stress or comfort conditions. Two groups of 20 birds (10 Hy-line W36 and 10 Hy-line Brown were submitted to two environmental conditions (26°C and 60% RH or 35°C and 70% RH and two housing systems (cages or litter in the early production phase. Egg quality was analyzed based on egg weight, eggshell thickness, specific gravity, and Haugh units. Yolk and shell contamination by Salmonella sp was also assessed. A significant (p<0.05 reduction in quality parameters was observed in eggs produced by laying hens under heat stress, mainly in the birds housed in cages.

  10. Study on Suitable Light Conditions and Efficient Lipid Extraction Technologies for Biodiesel Production Based on Microalgae

    Science.gov (United States)

    Wang, Yao; Zhang, Qingtao; Sun, Yuan; Yang, Chengjia

    2018-01-01

    As a new generation biodiesel feedstock, microalgae have most potential to replace fossil fuel. However, the limited scale and high cost are two bottleneck problems. Efficient microwave-assisted lipid extraction technologies and suitable light conditions for Chlorella Sorokiniana need further study for lowering the cost. In this study, three photoperiod groups(24L:0D, 12L:12D, 0L:24D), three illumination intensity groups (1800 lux, 3600 lux, 5400 lux)and four light spectrum groups (Red, green, blue, and white) were used to culture Chlorella Sorokiniana to investigate those effects on algae growth rate and biomass accumulation. The suitable microwave treatment was also studied to achieve an optimizing quantum fracturing technology. 400 w, 750 w and 1000 w microwave power were set and 60 °C, 75 °C, 90 °C microwave conditions were investigated. The results showed that Chlorella Sorokiniana under 24L:0D photoperiod with 5400 lux white light can achieve better growth rate. The 90 °C / 1000w microwave treatment was identified as the most simple, easy, and effective way for lipid extraction from Chlorella Sorokiniana. As the raw material of biodiesel production, C18:1, C18:2 and C18:3 have accounted for important components of fatty acid in Chlorella Sorokiniana. Therefore, Chlorella Sorokiniana is a good raw material for the production of good quality biodiesel under suitable and efficient technologies.

  11. Enhancement of Lutein Production in Chlorella sorokiniana (Chorophyta by Improvement of Culture Conditions and Random Mutagenesis

    Directory of Open Access Journals (Sweden)

    Maria Angeles Vargas

    2011-09-01

    Full Text Available Chlorella sorokiniana has been selected for lutein production, after a screening of thirteen species of microalgae, since it showed both a high content in this carotenoid and a high growth rate. The effects of several nutritional and environmental factors on cell growth and lutein accumulation have been studied. Maximal specific growth rate and lutein content were attained at 690 µmol photons m−2 s−1, 28 °C, 2 mM NaCl, 40 mM nitrate and under mixotrophic conditions. In general, optimal conditions for the growth of this strain also lead to maximal lutein productivity. High lutein yielding mutants of C. sorokiniana have been obtained by random mutagenesis, using N-methyl-N′-nitro-nitrosoguanidine (MNNG as a mutagen and selecting mutants by their resistance to the inhibitors of the carotenogenic pathway nicotine and norflurazon. Among the mutants resistant to the herbicides, those exhibiting both high content in lutein and high growth rate were chosen. Several mutants exhibited higher contents in this carotenoid than the wild type, showing, in addition, either a similar or higher growth rate than the latter strain. The mutant MR-16 exhibited a 2.0-fold higher volumetric lutein content than that of the wild type, attaining values of 42.0 mg L−1 and mutants DMR-5 and DMR-8 attained a lutein cellular content of 7.0 mg g−1 dry weight. The high lutein yield exhibited by C. sorokiniana makes this microalga an excellent candidate for the production of this commercially interesting pigment.

  12. Productive characteristics of age-3 brood brown trout (Salmo trutta reared in the conditions industrial aquaculture

    Directory of Open Access Journals (Sweden)

    L. Haloyan

    2017-03-01

    Full Text Available Purpose. Study and analysis of productive and reproductive parameters of brood brown trout reared in the conditions of industrial aquaculture with the use of feeding with specialized artificial feeds. Methodology. Fish rearing was performed using a technology traditional for rainbow trout. Biological evaluation of fish was performed on live material according to the scheme developed by I.F. Pravdin (1966 for salmonids. Fish were anesthetized with “Propiscin”. Metric parameters were measured with the aid of a ruler and caliper. Fish weight was determined on electronic balances. Eggs were preserved in a 4% formalin solution, weight of individual eggs was determined with the aid of torsion balances, egg diameter with a caliper. The duration of spermatozoid motility in males was determined under a microscope under 10X40 magnification with the aid of a stopwatch, sperm concentration was determined in a Goriaev chamber. Multiple regression analysis was performed in JMP IN 4 (SAS Institute. The factor was considered significant if р <0.05. Findings. Brood brown trout reared on specialized artificial feeds had following productive parameters: mean body weight of age-3 fish was 458 g in males and 453 g in females. Mean working fecundity of age-3 fish as 1847 eggs, mean relative working fecundity was 3971 eggs, the mean qualitative parameters of which were: weight of 61.5 g, diameter of 74.7 mm. The reproductive characteristics of age-3 males was high – mean fecundity was 1391 million sperm cells, their motility was 35 sec. Originality. For the first time we reared a stock of brood brown trout in industrial conditions with the use of feeding with specialized artificial feeds. Practical value. The obtained results are a component of the development of a complex technology of salmonid fish rearing with an objective to broad the assortment of valuable gourmet products and to justify the measures on artificial reproduction of brown trout in rivers of

  13. Optimal Condition of Fenton's Reagent to Enhance the Alcohol Production from Palm Oil Mill Effluent (POME

    Directory of Open Access Journals (Sweden)

    Supawadee Sinnaraprasat

    2011-07-01

    Full Text Available Application of Fenton's reaction for a proper hydrolysis step is an essential and important step in obtaining a higher level of readily biodegradable sugars from palm oil mill effluent (POME for improving the alcohol production by using immobilized Clostridium acetobutylicum. The objective of this research was, therefore, to investigate the optimum condition of Fenton's reaction in terms of COD: H2O2 ratios (w/w and H2O2: Fe2+ ratios (molar ratio used to oxidize carbohydrate and high molecular organic compounds into simple sugars, which are further fermented into alcohol. The experiments were carried out at H2O2: Fe2+ ratios (molar ratios of 5, 10, 20, 30 and 40 and the COD: H2O2 ratios (w/w of 50, 70, 100 and 130 (initial COD about 50,000 mg/L. The total sugar concentrations and organic compounds biodegradability (BOD5/COD ratios were also used for investigating suitable conditions for Fenton's reaction. The concentration of Fenton's reagent at H2O2:Fe2+ and COD:H2O2 ratio of 20 and 130 was identified as the optimum operating condition for the highest simple sugars of about 0.865% and BOD5/COD ratios of 0.539. The alcohol productions were carried out in the continuous stirred tank reactors (CSTR under an anaerobic continuous immobilization system. At a hydraulic retention time of 12 hours and POME pH of 4.8, the maximum total ABE concentration of 495 mg/L and the ABE yield of 0.236 grams of ABE produced/gram of reducing sugars were achieved at the mixed polyvinyl alcohol (PVA and palm oil ash (POA ratio of 10 : 3.

  14. Fission product release from HTGR fuel under core heatup accident conditions - HTR2008-58160

    International Nuclear Information System (INIS)

    Verfondern, K.; Nabielek, H.

    2008-01-01

    Various countries engaged in the development and fabrication of modern fuel for the High Temperature Gas-Cooled Reactor (HTGR) have initiated activities of modeling the fuel and fission product release behavior with the aim of predicting the fuel performance under operating and accidental conditions of future HTGRs. Within the IAEA directed Coordinated Research Project CRP6 on 'Advances in HTGR Fuel Technology Development' active since 2002, the 13 participating Member States have agreed upon benchmark studies on fuel performance during normal operation and under accident conditions. While the former has been completed in the meantime, the focus is now on the extension of the national code developments to become applicable to core heatup accident conditions. These activities are supported by the fact that core heatup simulation experiments have been resumed recently providing new, highly valuable data. Work on accident performance will be - similar to the normal operation benchmark - consisting of three essential parts comprising both code verification that establishes the correspondence of code work with the underlying physical, chemical and mathematical laws, and code validation that establishes reasonable agreement with the existing experimental data base, but including also predictive calculations for future heating tests and/or reactor concepts. The paper will describe the cases to be studied and the calculational results obtained with the German computer model FRESCO. Among the benchmark cases in consideration are tests which were most recently conducted in the new heating facility KUEFA. Therefore this study will also re-open the discussion and analysis of both the validity of diffusion models and the transport data of the principal fission product species in the HTGR fuel materials as essential input data for the codes. (authors)

  15. Methane production from acid hydrolysates of Agave tequilana bagasse: evaluation of hydrolysis conditions and methane yield.

    Science.gov (United States)

    Arreola-Vargas, Jorge; Ojeda-Castillo, Valeria; Snell-Castro, Raúl; Corona-González, Rosa Isela; Alatriste-Mondragón, Felipe; Méndez-Acosta, Hugo O

    2015-04-01

    Evaluation of diluted acid hydrolysis for sugar extraction from cooked and uncooked Agave tequilana bagasse and feasibility of using the hydrolysates as substrate for methane production, with and without nutrient addition, in anaerobic sequencing batch reactors (AnSBR) were studied. Results showed that the hydrolysis over the cooked bagasse was more effective for sugar extraction at the studied conditions. Total sugars concentration in the cooked and uncooked bagasse hydrolysates were 27.9 g/L and 18.7 g/L, respectively. However, 5-hydroxymethylfurfural was detected in the cooked bagasse hydrolysate, and therefore, the uncooked bagasse hydrolysate was selected as substrate for methane production. Interestingly, results showed that the AnSBR operated without nutrient addition obtained a constant methane production (0.26 L CH4/g COD), whereas the AnSBR operated with nutrient addition presented a gradual methane suppression. Molecular analyses suggested that methane suppression in the experiment with nutrient addition was due to a negative effect over the archaeal/bacterial ratio. Copyright © 2015. Published by Elsevier Ltd.

  16. Environmental conditions affecting exopolysaccharide production by Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp.

    Science.gov (United States)

    Kiliç, Nur Koçberber; Dönmez, Gönül

    2008-06-15

    Three different chromium-resistant microorganisms (Pseudomonas aeruginosa, Micrococcus sp., and Ochrobactrum sp.) were tested with regard to their EPS production at different pH levels, temperatures, Cr(VI) concentrations, and incubation periods. The optimum pH level was 7 for P. aeruginosa and Micrococcus sp., while it was 8 for Ochrobactrum sp. according to the highest EPS amount at 100 mg/L Cr(VI) concentration. The highest production of EPSs by the three bacteria was obtained under different environmental conditions. P. aeruginosa produced the highest EPS (863.3 mg/L) after incubation for 96 h on media with 50 mg/L Cr(VI) at 20 degrees C, Micrococcus sp. gave the highest yield (444.6 mg/L) after incubation for 72 h on media with 100 mg/L Cr(VI) at the same temperature, and Ochrobactrum sp. had the highest production (430.5 mg/L) on media with 150 mg/L Cr(VI) at 30 degrees C at the end of 48 h of incubation.

  17. Effect of substrate availability on nitrous oxide production by deammonification processes under anoxic conditions.

    Science.gov (United States)

    Schneider, Yvonne; Beier, Maike; Rosenwinkel, Karl-Heinz

    2012-05-01

    Due to its high global warming potential, nitrous oxide (N(2)O) emissions from wastewater treatment processes have recently received a high degree of attention. Nevertheless, there is still a lack of information regarding the microbiological processes leading to N(2)O production. In this study, two lab-scale sequencing batch reactors were operated with deammonification biomass to investigate the role of denitrification and the influence of substrate availability regarding N(2)O formation during the anoxic phase of deammonification. Three different operational phases were established: within the first phase conversion by anammox was favoured and after a transition phase, denitrification activity was promoted. Low nitrous oxide production was observed during stable operation aiming for anammox conversion. Pulsed inflow of the wastewater containing ammonium (NH(4)(+)) and nitrite (NO(2)(-)) led to increased N(2)O production rates. Within the period of denitrification as dominating nitrogen conversion process, the nitrous oxide concentration level was higher during continuous inflow conditions, but the reaction to pulsed inflow was less pronounced. The results indicated that denitrification was responsible for N(2)O formation from the deammonification biomass. Operational settings to achieve suppression of denitrification processes to a large extend were deducted from the results of the experiments. © 2012 The Authors. Microbial Biotechnology © 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.

  18. Fission product release in conditions of a spent fuel pool severe accident

    International Nuclear Information System (INIS)

    Ohai, Dumitru

    2007-01-01

    Full text: Depending on the residence time, fuel burnup, and fuel rack configuration, there may be sufficient decay heat for the fuel clad to heat up, swell, and burst in case of a loss of pool water. Initiating event categories can be: loss of offsite power from events initiated by severe weather, internal fire, loss of pool cooling, loss of coolant inventory, seismic event, aircraft impact, tornado, missile attack. The breach in the clad releases the radioactive gases present in the gap between the fuel and clad, what is called 'gap release'. If the fuel continues to heat up, the zirconium clad will reach the point of rapid oxidation in air. This reaction of zirconium and air, or zirconium and steam is exothermic. The energy released from the reaction, combined with the fuel's decay energy, can cause the reaction to become self-sustaining and ignite the zirconium. The increase in heat from the oxidation reaction can also raise the temperature in adjacent fuel assemblies and propagate the oxidation reaction. Simultaneously, the sintered UO 2 pellets resulting from pins destroying are oxidized. Due to the self-disintegration of pellets by oxidation, fission gases and low volatile fission products are released. The release rate, the chemical nature and the amount of fission products depend on powder granulation distribution and environmental conditions. The zirconium burning and pellets self-disintegration will result in a significant release of spent fuel fission products that will be dispersed from the reactor site. (author)

  19. A model for the release of low-volatility fission products in oxidizing conditions

    International Nuclear Information System (INIS)

    Cox, D.S.; Hunt, C.E.L.; Liu, Z.; Keller, N.A.; Barrand, R.D.; O'Connor, R.F.

    1991-07-01

    A thermodynamic and kinetic model has been developed for calculating low-volatility fission-product releases from UO 2 at high temperatures in oxidizing conditions. Volatilization of the UO 2 matrix is assumed to be the rate controlling process. Oxidation kinetics of the UO 2 are modelled by either interfacial rate control, gas phase oxidant transport control, or solid-state diffusion of oxygen. The vapour pressure of UO 3 in equilibrium with the oxidizing fuel is calculated from thermodynamic data, and volatilization rates are determined using a model for forced convective mass transport. Low-volatility fission-product releases are calculated from the volume of vapourized fuel. Model calculations are conservative compared to experimental data for Zr, La, Ce and Nb fission-product releases from irradiated UO 2 exposed to air at 1973-2350 K. The implications of this conservatism are discussed in terms of possible rate control by processes other than convective mass transport of UO 3 . Coefficients for effective surface area (based on experimental data) and for heterogeneous rate controlling reaction kinetics are introduced to facilitate agreement between calculations and the experimental data.

  20. Pressure retarded osmosis for energy production: membrane materials and operating conditions.

    Science.gov (United States)

    Kim, H; Choi, J-S; Lee, S

    2012-01-01

    Pressure retarded osmosis (PRO) is a novel membrane process to produce energy. PRO has the potential to convert the osmotic pressure difference between fresh water (i.e. river water) and seawater to electricity. Moreover, it can recover energy from highly concentrated brine in seawater desalination. Nevertheless, relatively little research has been undertaken for fundamental understanding of the PRO process. In this study, the characteristics of the PRO process were examined using a proof-of-concept device. Forward osmosis (FO), reverse osmosis (RO), and nanofiltration (NF) membranes were compared in terms of flux rate and concentration polarization ratio. The results indicated that the theoretical energy production by PRO depends on the membrane type as well as operating conditions (i.e. back pressure). The FO membrane had the highest energy efficiency while the NF membrane had the lowest efficiency. However, the energy production rate was low due to high internal concentration polarization (ICP) in the PRO membrane. This finding suggests that the control of the ICP is essential for practical application of PRO for energy production.

  1. Continuous production of biodiesel under supercritical methyl acetate conditions: Experimental investigation and kinetic model.

    Science.gov (United States)

    Farobie, Obie; Matsumura, Yukihiko

    2017-10-01

    In this study, biodiesel production by using supercritical methyl acetate in a continuous flow reactor was investigated for the first time. The aim of this study was to elucidate the reaction kinetics of biodiesel production by using supercritical methyl. Experiments were conducted at various reaction temperatures (300-400°C), residence times (5-30min), oil-to-methyl acetate molar ratio of 1:40, and a fixed pressure of 20MPa. Reaction kinetics of biodiesel production with supercritical methyl acetate was determined. Finally, biodiesel yield obtained from this method was compared to that obtained with supercritical methanol, ethanol, and MTBE (methyl tertiary-butyl ether). The results showed that biodiesel yield with supercritical methyl acetate increased with temperature and time. The developed kinetic model was found to fit the experimental data well. The reactivity of supercritical methyl acetate was the lowest, followed by that of supercritical MTBE, ethanol, and methanol, under the same conditions. Copyright © 2017. Published by Elsevier Ltd.

  2. Optimization of enzymatic hydrolysis and fermentation conditions for improved bioethanol production from potato peel residues.

    Science.gov (United States)

    Ben Taher, Imen; Fickers, Patrick; Chniti, Sofien; Hassouna, Mnasser

    2017-03-01

    The aim of this work was the optimization of the enzyme hydrolysis of potato peel residues (PPR) for bioethanol production. The process included a pretreatment step followed by an enzyme hydrolysis using crude enzyme system composed of cellulase, amylase and hemicellulase, produced by a mixed culture of Aspergillus niger and Trichoderma reesei. Hydrothermal, alkali and acid pretreatments were considered with regards to the enhancement of enzyme hydrolysis of potato peel residues. The obtained results showed that hydrothermal pretreatment lead to a higher enzyme hydrolysis yield compared to both acid and alkali pretreatments. Enzyme hydrolysis was also optimized for parameters such as temperature, pH, substrate loading and surfactant loading using a response surface methodology. Under optimized conditions, 77 g L -1 of reducing sugars were obtained. Yeast fermentation of the released reducing sugars led to an ethanol titer of 30 g L -1 after supplementation of the culture medium with ammonium sulfate. Moreover, a comparative study between acid and enzyme hydrolysis of potato peel residues was investigated. Results showed that enzyme hydrolysis offers higher yield of bioethanol production than acid hydrolysis. These results highlight the potential of second generation bioethanol production from potato peel residues treated with onsite produced hydrolytic enzymes. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:397-406, 2017. © 2017 American Institute of Chemical Engineers.

  3. Butanol production under microaerobic conditions with a symbiotic system of Clostridium acetobutylicum and Bacillus cereus.

    Science.gov (United States)

    Wu, Pengfei; Wang, Genyu; Wang, Gehua; Børresen, Børre Tore; Liu, Hongjuan; Zhang, Jianan

    2016-01-14

    One major problem of ABE (acetone, butanol and ethanol) fermentation is high oxygen sensitivity of Clostridium acetobutylicum. Currently, no single strain has been isolated or genetically engineered to produce butanol effectively under aerobic conditions. In our previous work, a symbiotic system TSH06 has been developed successfully by our group, and two strains, C. acetobutylicum TSH1 and Bacillus cereus TSH2, were isolated from TSH06. Compared with single culture, TSH06 showed promotion on cell growth and solvent accumulation under microaerobic conditions. To simulate TSH06, a new symbiotic system was successfully re-constructed by adding living cells of B. cereus TSH2 into C. acetobutylicum TSH1 cultures. During the fermentation process, the function of B. cereus TSH2 was found to deplete oxygen and provide anaerobic environment for C. acetobutylicum TSH1. Furthermore, inoculation ratio of C. acetobutylicum TSH1 and B. cereus TSH2 affected butanol production. In a batch fermentation with optimized inoculation ratio of 5 % C. acetobutylicum TSH1 and 0.5 % B. cereus TSH2, 11.0 g/L butanol and 18.1 g/L ABE were produced under microaerobic static condition. In contrast to the single culture of C. acetobutylicum TSH1, the symbiotic system became more aerotolerant and was able to produce 11.2 g/L butanol in a 5 L bioreactor even with continuous 0.15 L/min air sparging. In addition, qPCR assay demonstrated that the abundance of B. cereus TSH2 increased quickly at first and then decreased sharply to lower than 1 %, whereas C. acetobutylicum TSH1 accounted for more than 99 % of the whole population in solventogenic phase. The characterization of a novel symbiotic system on butanol fermentation was studied. The new symbiotic system re-constructed by co-culture of C. acetobutylicum TSH1 and B. cereus TSH2 showed excellent performance on butanol production under microaerobic conditions. B. cereus TSH2 was a good partner for C. acetobutylicum TSH1 by providing an anaerobic

  4. Assessment of biogas production from MBT waste under different operating conditions

    Energy Technology Data Exchange (ETDEWEB)

    Pantini, Sara, E-mail: pantini@ing.uniroma2.it [Department of Civil Engineering and Computer Science Engineering, University of Rome “Tor Vergata”, Via del Politecnico, 1, 00133 Rome (Italy); Verginelli, Iason; Lombardi, Francesco [Department of Civil Engineering and Computer Science Engineering, University of Rome “Tor Vergata”, Via del Politecnico, 1, 00133 Rome (Italy); Scheutz, Charlotte; Kjeldsen, Peter [Department of Environmental Engineering, Technical University of Denmark, Miljoevej, Building 113, DK-2800 Kgs. Lyngby (Denmark)

    2015-09-15

    Highlights: • BMP test displayed high gas potential generation capacity of MBT waste. • Strong inhibition effects were observed due to ammonia and VFA accumulation. • Waste water content was found as the key parameter limiting gas generation. • First order k-values were determined for different operating conditions. - Abstract: In this work, the influence of different operating conditions on the biogas production from mechanically–biologically treated (MBT) wastes is investigated. Specifically, different lab-scale anaerobic tests varying the water content (26–43% w/w up to 75% w/w), the temperature (from 20 to 25 °C up to 55 °C) and the amount of inoculum have been performed on waste samples collected from a full-scale Italian MBT plant. For each test, the gas generation yield and, where applicable, the first-order gas generation rates were determined. Nearly all tests were characterised by a quite long lag-phase. This result was mainly ascribed to the inhibition effects resulting from the high concentrations of volatile fatty acids (VFAs) and ammonia detected in the different stages of the experiments. Furthermore, water content was found as one of the key factor limiting the anaerobic biological process. Indeed, the experimental results showed that when the moisture was lower than 32% w/w, the methanogenic microbial activity was completely inhibited. For the higher water content tested (75% w/w), high values of accumulated gas volume (up to 150 Nl/kgTS) and a relatively short time period to deplete the MBT waste gas generation capacity were observed. At these test conditions, the effect of temperature became evident, leading to gas generation rates of 0.007 d{sup −1} at room temperature that increased to 0.03–0.05 d{sup −1} at 37 °C and to 0.04–0.11 d{sup −1} at 55 °C. Overall, the obtained results highlighted that the operative conditions can drastically affect the gas production from MBT wastes. This suggests that particular caution

  5. Impact of cooking and handling conditions on furanic compounds in breaded fish products.

    Science.gov (United States)

    Pérez-Palacios, T; Petisca, C; Henriques, R; Ferreira, I M P L V O

    2013-05-01

    This study evaluates the influence of cooking and handling conditions on the quantity of furanic compounds (furan, 2-furfural, furfuryl alcohol, 2-pentylfuran, 5-hydroxymethylfurfural) in breaded fish products. Oven-baking and reheating in the microwave lead to low furanic compounds formation in comparison with deep-frying. The use of olive oil for deep-frying promoted higher levels of furanic compounds than sunflower oil. The amounts of these compounds diminished as the temperature and time of deep-frying decreased as well as after a delay after deep-frying. Thus, the generation of furanic compounds can be minimized by adjusting the cooking method and conditions, such as using an electric oven, deep-frying in sunflower oil at 160°C during 4min, or waiting 10min after cooking. However, these conditions that reduce furanic compounds levels also reduce the content of volatile compounds related to the aroma and flavour of fried foods. In this sense, new efforts should be done to reduce the formation of furanic compounds without being detrimental to the volatile profile. Copyright © 2013 Elsevier Ltd. All rights reserved.

  6. Effective donor cell fusion conditions for production of cloned dogs by somatic cell nuclear transfer.

    Science.gov (United States)

    Park, JungEun; Oh, HyunJu; Hong, SoGun; Kim, MinJung; Kim, GeonA; Koo, OkJae; Kang, SungKeun; Jang, Goo; Lee, ByeongChun

    2011-03-01

    As shown by the birth of the first cloned dog 'Snuppy', a protocol to produce viable cloned dogs has been reported. In order to evaluate optimum fusion conditions for improving dog cloning efficiency, in vivo matured oocytes were reconstructed with adult somatic cells from a female Pekingese using different fusion conditions. Fusion with needle vs chamber methods, and with low vs high pulse strength was compared by evaluating fusion rate and in vivo development of canine cloned embryos. The fusion rates in the high voltage groups were significantly higher than in the low voltage groups regardless of fusion method (83.5 vs 66.1% for the needle fusion method, 67.4 vs 37.9% for the fusion chamber method). After embryo transfer, one each pregnancy was detected after using the needle fusion method with high and low voltage and in the chamber fusion method with high voltage, whereas no pregnancy was detected using the chamber method with low voltage. However, only the pregnancy from the needle fusion method with high voltage was maintained to term and one healthy puppy was delivered. The results of the present study demonstrated that two DC pulses of 3.8 to 4.0 kV/cm for 15 μsec using the needle fusion method were the most effective method for the production of cloned dogs under the conditions of this experiment. Copyright © 2011 Elsevier Inc. All rights reserved.

  7. Effect of silicon and selenium on enzymatic changes and productivity of dill in saline condition

    Directory of Open Access Journals (Sweden)

    Fariborz Shekari

    2017-10-01

    Full Text Available Anethum graveolens is an annual herb in the celery family Apiaceae. The experiment was carried out in a factorial design with two factors include salinity, which was applied to the root medium as NaCl (0 and 10 ds/m and nutrition as sodium silicate (0 and 1.5 mM, and selenate (0, 5 μM. Supplementary Si or Se ameliorated the negative effects of salinity on plant dry matter and chlorophyll content. Application of Si or Se decreased Na+ concentration and increased K+ concentration in roots and shoots of dill plants. Salinity imposed oxidative stress and leaded to increase malondialdehyde (MDA concentration. Under saline condition, addition of Si/Se significantly increased the activities of superoxide dismutase (SOD and catalase (CAT in salt-stressed plant when compared with plant subjected to salinity alone. Our results revealed that improvement in growth of salt stressed plants under the influence of Si and Se may be due to the improved ion balance, antioxidant enzymes activities and osmotic adjustment. These trace elements had negative effect on growth under non-saline conditions. Therefore, application of these trace elements (especially Silicon under saline condition could be a better strategy for maintaining the crop productivity in these regions.

  8. Sewage sludge hydrochars: properties and agronomic impact as related to different production conditions

    Science.gov (United States)

    Paneque, Marina; María De la Rosa, José; Aragón, Carlos; Kern, Jürgen; Conte, Pellegrino; Knicker, Heike

    2015-04-01

    The huge amount of sewage sludge (SS) generated in wastewater treatment plants all over the world represents an environmental problem. Due to the high concentration of phosphorus and nitrogen in SS as well as other macro and micro nutrients it has been considered a suitable soil amendment. However, before being applied to soil a complete sterilization and elimination of pollutants should be carried out [1]. In this context, thermal treatments appear as a convenient methodology for producing SS byproducts useful for agronomic purposes. Hydrothermal carbonization (HTC) is a kind of pyrolysis characterized by the heating of the biomass in presence of water. This process shows an advantage compared to other thermal treatments for wet residues since dryness of the biomass prior to the thermal transformation is not necessary. The solid product which results from HTC is called hydrochar and it has been suggested to increase soil productivity [2]. However, the agronomic potential of hydrochars depends on the feedstock and production conditions. Additionally, possible toxic and risks have to be carefully evaluated. Thus, SS hydrochars appear as a potential soil amendment but further scientific research is needed to find its real capacity, optimal production conditions as well as possible environmental harmful effects. The aim of this study was to evaluate which are the most suitable production conditions, to transform SS into hydrochar. An additional goal of this work was to relate the hydrochars properties to its agronomic response. Therefore, hydrochars were produced from SS collected from the Experimental Wastewater Treatment plant of CENTA (http://www.centa.es/), located in Carrion de los Cespedes (Seville), under two different temperatures (200 and 260˚C) and residence times (30 min and 1h). With the hydrochars obtained, a greenhouse pot incubation study was carried out for 80 days. The pots contained 250 g of a Calcic Cambisol (IUSS Working Group WRB, 2007) and an

  9. Determination and evaluation of the best conditions for the production of a vaccine against Clostridium chauvoei

    Directory of Open Access Journals (Sweden)

    Jorge Humberto Ossa

    2005-05-01

    Full Text Available Clostridium chauvoei is responsible for a number of diseases such as symptomatic coal (blackleg and malignant oedema in cattle, sheep and many other domestic and wild animals. Vaccines for the control of this and other livestock diseases caused by various species of clostridia are widely used. For vaccine production it is desirable that high cellular density cultures with high immunogenic power are obtained, mainly because immunity to Clostridium chauvoei is generally considered to be antibacterial rather than antitoxic. Consequently, culture conditions have to be adequately monitored and controlled. The aim of this work was to optimise the production of a vaccine against Clostridium chauvoei based on strains IRP-434, IRP 206, ATCC 10092, ATCC 11957 and LANIP, used by “Empresa Colombiana de Productos Veterinarios”, VECOL, S.A., to determine the culture medium that allows the highest cellular density. Results show that modified clostridium medium allows the highest cellular density production with average values of 31,5X108 cell/ml for the stock IRP 434 and 31,8X108 cell/ml for the stock IRP 206. Once defined the modified clostridium medium, the stock IRP 434,IRP 206 and growth time as the most suitable for optimal biomass production ( 16 – 48 hours, the lethal activity of the stock (LD50 = 106.6 , 106.8 was determined, the immunogenicity was evaluated through a potency test carried out in guinea-pigs. The presence of the flagellar protein was confirmed through the SDS-PAGE electrophoresis technique.

  10. Fermentation conditions that affect clavulanic acid production in Streptomyces clavuligerus: a systematic review

    Directory of Open Access Journals (Sweden)

    Hooi-Leng eSer

    2016-04-01

    Full Text Available The β-lactamase inhibitor, clavulanic acid is frequently used in combination with β-lactam antibiotics to treat a wide spectrum of infectious diseases. Clavulanic acid prevents drug resistance by pathogens against these β-lactam antibiotics by preventing the degradation of the β-lactam ring, thus ensuring eradication of these harmful microorganisms from the host. This systematic review provides an overview on the fermentation conditions that affect the production of clavulanic acid in the firstly described producer, Streptomyces clavuligerus. A thorough search was conducted using predefined terms in several electronic databases (PubMed, Medline, ScienceDirect, EBSCO, from database inception to June 30th 2015. Studies must involve wild-type Streptomyces clavuligerus, and full texts needed to be available. A total of 29 eligible articles were identified. Based on the literature, several factors were identified that could affect the production of clavulanic acid in S. clavuligerus. The addition of glycerol or other vegetable oils (e.g. olive oil, corn oil could potentially affect clavulanic acid production. Furthermore, some amino acids such as arginine and ornithine, could serve as potential precursors to increase clavulanic acid yield. The comparison of different fermentation systems revealed that fed-batch fermentation yields higher amounts of clavulanic acid as compared to batch fermentation, probably due to the maintenance of substrates and constant monitoring of certain entities (such as pH, oxygen availability, etc.. Overall, these findings provide vital knowledge and insight that could assist media optimization and fermentation design for clavulanic acid production in S. clavuligerus.

  11. Suppression of multiple bioactivities of interleukin-1 and interleukin-2 production by U937 conditioned medium

    International Nuclear Information System (INIS)

    Wiblin, R.T.; Edmonds, K.; Ellner, J.J.

    1986-01-01

    The human macrophage-like cell line U937 spontaneously produces a factor which blocks interleukin-1 (IL-1) activity for mouse thymocytes but not mitosis of T-lymphoblastoid cells. The authors examined the effects of U937 conditioned medium (CM) on other IL-1 activities and on interleukin-2 (IL-2) production. U937 was cultured at 5 x 10 6 /ml in RPMI-1640 at 37 0 C for 5 days. The resulting CM inhibited the mitogenic response of C3H/HeJ mouse thymocytes to an IL-1 standard, with an inhibitory of activity of 6.64 U/ml (1 U = reciprocal dilution producing 50% inhibition of maximal response). Similarly, CM inhibited (10.12 U/ml) the fibroblast stimulation promoter activity of IL-1. The effect of CM on IL-2 production was assessed in a direct assay in which IL-2 production by γ-irradiated (12,000 rads) MLA-144 lymphosarcoma cells was assayed as 3 H-thymidine incorporation in CTLL-20 cells. The suppressive activity of CM was 4.95 U/ml; CM did not interfere with the response of CTLL-20 to IL-2. These studies establish that U937 produces factors with multiple, related biological activities; U937 CM blocks IL-2 dependent (thymocyte mitogenesis) and IL-2 independent (fibroblast proliferation) IL-1 activities and interferes with production of, but not response to, IL-2. U937 is an excellent model to study growth inhibitory properties of mononuclear phagocytes

  12. Biogas production from high-yielding energy crops in boreal conditions

    Energy Technology Data Exchange (ETDEWEB)

    Seppala, M.

    2013-11-01

    In this thesis, the methane production potential of traditional and novel energy crops was evaluated in boreal conditions. The highest methane yield per hectare was achieved with maize (4 000-9 200 m{sup 3}CH{sub 4} ha{sup -1} a{sup -1}) and the second highest with brown knapweed (2 700-6 100 m{sup 3}CH{sub 4} ha{sup -1} a{sup -1}). Recently, the most feasible energy crop, grass, produced 1 200-3 600 m{sup 3}CH{sub 4} ha{sup -1} a{sup -1}. The specific methane yields of traditional and novel energy crops varied from 170-500 l kg{sup -1} volatile solid (VS). The highest specific methane yields were obtained with maize, while the novel energy crops were at a lower range. The specific methane yields decreased in the later harvest time with maize and brown knapweed, and the specific methane yield of the grasses decreased from the 1st to 2nd harvests. Maize and brown knapweed produced the highest total solid (TS) yields per hectare 13-23 tTS ha{sup -1}, which were high when compared with the TS yields of grasses (6-13 tTS ha{sup -1}). The feasibility of maize and brown knapweed in co-digestion with liquid cow manure, in continuously stirred tank reactors (CSTR), was evaluated. According to the CSTR runs, maize and brown knapweed are suitable feeds and have stable processes, producing the highest methane yields (organic loading rate 2 kgVS m{sup -3}d{sup -1}), with maize at 259 l kgVS{sup -1} and brown knapweed at 254 l kgVS{sup -1}. The energy balance (input/output) of the cultivation of the grasses, maize and brown knapweed was calculated in boreal conditions, and it was better when the digestate was used as a fertilizer (1.8-4.8 %) than using chemical fertilizers (3.7-16.2 %), whose production is the most energy demanding process in cultivation. In conclusion, the methane production of maize, grasses and novel energy crops can produce high methane yields and are suitable feeds for anaerobic digestion. The cultivation managements of maize and novel energy crops for

  13. Assessing potential changes of chestnut productivity in Europe under future climate conditions

    Science.gov (United States)

    Calheiros, T.; Pereira, M. G.; Pinto, J. G.; Caramelo, L.; Gomes-Laranjo, J.; Dacamara, C. C.

    2012-04-01

    The European chestnut is cultivated for its nuts and wood. Several studies point to the dependency of chestnut productivity on specific soil and climate characteristics. For instance, this species dislikes chalky and poorly drained soils, appreciates sedimentary, siliceous and acidic to neutral soils. Chestnut trees also seems to appreciate annual mean values of sunlight spanning between 2400 and 2600 h, rainfall ranging between 600 and 1500 mm, mean annual temperature between 9 and 13°C, 27°C being the mean of the maximum temperature (Heiniger and Conedera, 1992; Gomes-Laranjo et al.,2008). The amount of heat between May and October must range between 1800°D and 2400°D (Dinis et al., 2011) . In Poland, the growing season is defined as the period of time when the mean 24-h temperature is greater than 5°C (Wilczynski and Podalski, 2007). In Portugal, maximum photosynthetic activity occurs at 24-28°C for adult trees, but exhibits more than 50% of termoinhibition when the air temperature is above 32°C, which is frequent during summer (Gomes- Laranjo et al., 2006, 2008). Recently Pereira et al (2011) identified a set of meteorological variables/parameters with high impact on chestnut productivity. The main purpose of this work is to assess the potential impacts of future climate change on chestnut productivity in Portugal as well as on European chestnut orchards. First, observed data from the European Climate assessment (ECA) and simulations with the Regional Circulation Model (RCM) COSMO-CLM for recent climate conditions are used to assess the ability of the RCM to model the actual meteorological conditions. Then, ensemble projections from the ECHAM5/COSMO-CLM model chain for two climate scenarios (A1B and B1) are used to estimate the values of relevant meteorological variables and parameters und future climate conditions. Simulated values are then compared with those obtained for present climate. Results point to changes in the spatial and temporal

  14. Effect of gamma irradiation, culture conditions and media composition on metallothionein production by Bacillus pantothenticus

    International Nuclear Information System (INIS)

    Tawfik, Z.S.; Swailam, H.M.; EL-Sonbaty, S.M.; Sayed, M.A.

    2010-01-01

    Metallothioneins (MTs) are cystine rich proteins found in all living organisms and play important roles as a radical scavenger and in metal homeostasis. Their optimum culture conditions and media composition of B. pantothenticuszn and B.pantothenticuscu were 1.5 g/L maltose and 1.5 g/l lactose as media carbon sources respectively, 48 hrs incubation period, 35 degree C, ph 8, 200 r.p.m. agitation speed, 26 g/L ammonium dihydrogen orthophosphate as nitrogen source, 0.5 g/L cysteine, 6% of 2.5x107 c.f.u./ml. inoculum size and exposure to a level dose of 4 kGy of gamma radiation. All the previous parameters increased the production of MT by B. pantothenticuszn and B.pantothenticuscu strains 12 and 10 times, respectively compared to the parent strains

  15. Entropy Production and Equilibrium Conditions of General-Covariant Spin Systems

    Directory of Open Access Journals (Sweden)

    Wolfgang Muschik

    2015-12-01

    Full Text Available In generalizing the special-relativistic one-component version of Eckart’s continuum thermodynamics to general-relativistic space-times with Riemannian or post-Riemannian geometry as presented by Schouten (Schouten, J.A. Ricci-Calculus, 1954 and Blagojevic (Blagojevic, M. Gauge Theories of Gravitation, 2013 we consider the entropy production and other thermodynamical quantities, such as the entropy flux and the Gibbs fundamental equation. We discuss equilibrium conditions in gravitational theories, which are based on such geometries. In particular, thermodynamic implications of the non-symmetry of the energy-momentum tensor and the related spin balance equations are investigated, also for the special case of general relativity.

  16. Controlled release of volatiles under mild reaction conditions: from nature to everyday products.

    Science.gov (United States)

    Herrmann, Andreas

    2007-01-01

    Volatile organic compounds serve in nature as semiochemicals for communication between species, and are often used as flavors and fragrances in our everyday life. The quite limited longevity of olfactive perception has led to the development of pro-perfumes or pro-fragrances--ideally nonvolatile and odorless fragrance precursors which release the active volatiles by bond cleavage. Only a limited amount of reaction conditions, such as hydrolysis, temperature changes, as well as the action of light, oxygen, enzymes, or microorganisms, can be used to liberate the many different chemical functionalities. This Review describes the controlled chemical release of fragrances and discusses additional challenges such as precursor stability during product storage as well as some aspects concerning toxicity and biodegradability. As the same systems can be applied in different areas of research, the scope of this Review covers fragrance delivery as well as the controlled release of volatiles in general.

  17. SUFFERING: THE ADVERSE MEANING OF WORK CONDITIONS AT THE FLEXIBLE PRODUCTION SYSTEM

    Directory of Open Access Journals (Sweden)

    SILMARA CIMBALISTA

    2007-01-01

    Full Text Available This article reports the vision, the consciousness, and the tolerance of workers at the assembly line in automotiveindustries and the car supply industry sited at the Metropolitan area in Curitiba (RMC, trying to demonstrate theirdaily strife at work. It explains about the flexible production work organization and the conditions of work inside theactivities developed at the work quotidian and the effects over workers’ physical and subjective dimensions. The worksituations reported try to demonstrate some kinds of suffering or physical and mental deterioration created throughoutthe work. The data and workers’ reports are from interviews performed as a part of my doctorate thesis. In spite of theresearcher’s difficulties so as to not express feelings before the workers’ narrative, we got to interpret them with the helpof sociology of work and organizational behaviour authors.

  18. Biodiesel production by esterification of oleic acid with short-chain alcohols under ultrasonic irradiation condition

    Energy Technology Data Exchange (ETDEWEB)

    Hanh, Hoang Duc; Okitsu, Kenji; Nishimura, Rokuro; Maeda, Yasuaki [Department of Applied Material Science, Graduate School of Engineering, Osaka Prefecture University, Gakuen-cho 1-1, Sakai, Osaka 599-8531 (Japan); Dong, Nguyen The [Institute of Environmental Technology, Vietnamese Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi (Viet Nam)

    2009-03-15

    Production of fatty acid ethyl ester (FAEE) from oleic acid (FFA) with short-chain alcohols (ethanol, propanol, and butanol) under ultrasonic irradiation was investigated in this work. Batch esterification of oleic acid was carried out to study the effect of: test temperatures of 10-60 C, molar ratios of alcohol to oleic acid of 1:1-10:1, quantity of catalysts of 0.5-10% (wt of sulfuric acid/wt of oleic acid) and irradiation times of 10 h. The optimum condition for the esterification process was molar ratio of alcohol to oleic acid at 3:1 with 5 wt% of H{sub 2}SO{sub 4} at 60 C with an irradiation time of 2 h. (author)

  19. Effect of ultraviolet rays upon the productivity and adaptive possibilities of sows raised under industrial conditions

    International Nuclear Information System (INIS)

    Stefanova, S.; Benkov, M.; Panev, P.; Zlatev, D.

    1983-01-01

    Two experiments, aimed at establishing the effect of treatment with UV-rays on productivity of sows, the growth rate of pigs up to weaning, their adaptive reactions, and the micro-climate on the premises were carried out with 60 sows, divided into two groups: group i (controls) raised on premises without treatment with UV-rays, and group ii (experimental) raised on premises under treatment with UV-rays. Both nursing sows and piglets until their weaning were raised under industrial conditions. Type PRK-2 mercury lamp of 120 V, 3.75 A, and 375 W was used as a source of UV-rays. Each animal was exposed to irradiation daily for a period of 2 min. The live weight of sows, prolificacy, live weight of pigs at weaning and number of culled pigs during the sucking period, were checked. The adaptive reactions of sows and pigs were examined by ACT-hormon testing. Before treatment and 3 h after treatment with this hormone leucocyte, eosinophile and erythrocyte count and the haemoglobin, Ca, blood sugar, total protein, albumin and globulin in the blood were checked. The temperature, moisture, velocity of movement and gas composition of air were measured prior to blood sampling. Under the conditions existing in industrial pig-breeding complexes ensuring complete feeding of animals UV-treatment did not produce essential effects on the productivity of sows and the growth rate of pigs as well as on the adaptive reactions of animals and the micro-climate on the premises

  20. Effect of ultraviolet rays upon the productivity and adaptive possibilities of sows raised under industrial conditions

    Energy Technology Data Exchange (ETDEWEB)

    Stefanova, S; Benkov, M; Panev, P; Zlatev, D [Pig-Breeding Research Institute, Shumen (Bulgaria)

    1983-01-01

    Two experiments, aimed at establishing the effect of treatment with UV-rays on productivity of sows, the growth rate of pigs up to weaning, their adaptive reactions, and the micro-climate on the premises were carried out with 60 sows, divided into two groups: group i (controls) raised on premises without treatment with UV-rays, and group ii (experimental) raised on premises under treatment with UV-rays. Both nursing sows and piglets until their weaning were raised under industrial conditions. Type PRK-2 mercury lamp of 120 V, 3.75 A, and 375 W was used as a source of UV-rays. Each animal was exposed to irradiation daily for a period of 2 min. The live weight of sows, prolificacy, live weight of pigs at weaning and number of culled pigs during the sucking period, were checked. The adaptive reactions of sows and pigs were examined by ACTH-hormone testing. Before treatment and 3 h after treatment with this hormone leucocyte, eosinophile and erythrocyte count and the hemoglobin, Ca, blood sugar, total protein, albumin and globulin in the blood were checked. The temperature, moisture, velocity of movement and gas composition of air were measured prior to blood sampling. Under the conditions existing in industrial pig-breeding complexes ensuring complete feeding of animals UV-treatment did not produce essential effects on the productivity of sows and the growth rate of pigs as well as on the adaptive reactions of animals and the micro-climate on the premises.

  1. Optimization of the growth conditions for amylase production by bacillus licheniformis 208 isolated from local hotsprings of karachi

    International Nuclear Information System (INIS)

    Asad, W.; Saleem, F.; Ajaz, M.; Rasool, S.A.

    2014-01-01

    Studies on the optimum conditions for the production of extracellular amylase were carried out with a newly isolated strain of Bacillus 208 from the hotsprings in Karachi. The optimum temperature, initial medium pH and incubation period for amylase production were 50 degree C, 7.0 and 24 hrs respectively. Furthermore, cells when grown in the complex media showed high amylase production compared to the minimal medium. Effect of different carbon sources revealed that soluble starch (1%) increased the amylase yield significantly. Peptone (as nitrogen source) gave higher yield as compared to other nitrogen sources tested. Under optimized conditions, the organism entered the stationary phase after 12 hrs and amylase production was observed to be maximum at 24th hrs of cultivation. Enzyme production regulation is influenced by catabolite repression. Reduction in enzyme production was observed in the presence of EDTA while addition of tween 20 and CaCl/sub 2/ helped to enhance the enzyme production. (author)

  2. Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome

    Science.gov (United States)

    Nolla-Ardèvol, Vímac; Strous, Marc; Tegetmeyer, Halina E.

    2015-01-01

    A haloalkaline anaerobic microbial community obtained from soda lake sediments was used to inoculate anaerobic reactors for the production of methane rich biogas. The microalga Spirulina was successfully digested by the haloalkaline microbial consortium at alkaline conditions (pH 10, 2.0 M Na+). Continuous biogas production was observed and the obtained biogas was rich in methane, up to 96%. Alkaline medium acted as a CO2 scrubber which resulted in low amounts of CO2 and no traces of H2S in the produced biogas. A hydraulic retention time (HRT) of 15 days and 0.25 g Spirulina L−1 day−1 organic loading rate (OLR) were identified as the optimal operational parameters. Metagenomic and metatranscriptomic analysis showed that the hydrolysis of the supplied substrate was mainly carried out by Bacteroidetes of the “ML635J-40 aquatic group” while the hydrogenotrophic pathway was the main producer of methane in a methanogenic community dominated by Methanocalculus. PMID:26157422

  3. Optimization on Pretreatment Conditions of Seaweed Liquid Waste for Bio ethanol Production

    International Nuclear Information System (INIS)

    Nur Zatul-Iffah Zakaria; Dachyar Arbain; Mohd Noor Ahmad; Mohd Irfan Hatim Mohamed Dzahir

    2015-01-01

    Seaweed liquid waste (SLW) from a non-conventional seaweed (Gracilaria sp.) drying process where the seaweed is ruptured and filter-squeezed has been investigated. The liquid contains proteins and minerals which potentially pollute the environment if it is not been properly treated. For that reason, this paper deals with study on the feasibility of SLW utilization as a feedstock for bio ethanol production. The fermentation of bio ethanol production was carried out by Saccharomyces cerevisiae in which ethanol produced was measured by gas chromatography. In order to increase its fermentable sugar content, the SLW was treated with dilute acid. Center composite design of response surface methodology (RSM) had been used to optimize the sugar content by varying the parameters involved in the dilute acid pretreatment conditions. These are sulphuric acid concentration (M), temperature (degree Celsius) and seaweed waste concentration (g/ ml). It was obtained that the R 2 value reached 0.97 indicating that the model is acceptable. The three parameters showed p-value less than 0.05 suggesting their significance interactions. The optimization resulted 25 times improvement of reducing sugar concentration. The reducing sugar resulting from the optimized pretreatment was later used as fermentation medium to produce ethanol up to 123.197 mg/ l. (author)

  4. Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15

    Directory of Open Access Journals (Sweden)

    El-Sayed E. Mostafa

    2012-01-01

    Full Text Available Microbial protease represents the most important industrial enzymes, which have an active role in biotechnological processes. The objective of this study was to isolate new strain of Streptomyces that produce proteolytic enzymes with novel properties and the development of the low-cost medium. An alkaline protease producer strain NRC-15 was isolated from Egyptian soil sample. The cultural, morphological, physiological characters and chemotaxonomic evidence strongly indicated that the NRC-15 strain represents a novel species of the genus Streptomyces, hence the name Strptomyces pseudogrisiolus NRC-15. The culture conditions for higher protease production by NRC-15 were optimized with respect to carbon and nitrogen sources, metal ions, pH and temperature. Maximum protease production was obtained in the medium supplemented with 1% glucose, 1% yeast extract, 6% NaCl and 100 μmol/L of Tween 20, initial pH 9.0 at 50 °C for 96 h. The current results confirm that for this strain, a great ability to produce alkaline proteases, which supports the use of applications in industry.

  5. Continuous production of biodiesel from microalgae by extraction coupling with transesterification under supercritical conditions.

    Science.gov (United States)

    Zhou, Dan; Qiao, Baoquan; Li, Gen; Xue, Song; Yin, Jianzhong

    2017-08-01

    Raw material for biodiesel has been expanded from edible oil to non-edible oil. In this study, biodiesel continuous production for two kinds of microalgae Chrysophyta and Chlorella sp. was conducted. Coupling with the supercritical carbon dioxide extraction, the oil of microalgae was extracted firstly, and then sent to the downstream production of biodiesel. The residue after decompression can be reused as the material for pharmaceuticals and nutraceuticals. Results showed that the particle size of microalgae, temperature, pressure, molar ration of methanol to oil, flow of CO 2 and n-hexane all have effects on the yield of biodiesel. With the optimal operation conditions: 40mesh algae, extraction temperature 60°C, flow of n-hexane 0.4ml/min, reaction temperature: 340°C, pressure: 18-20MPa, CO 2 flow of 0.5L/min, molar ration of methanol to oil 84:1, a yield of 56.31% was obtained for Chrysophyta, and 63.78% for Chlorella sp. due to the higher lipid content. Copyright © 2017. Published by Elsevier Ltd.

  6. Degradation of materials under conditions of thermochemical cycles for hydrogen production

    International Nuclear Information System (INIS)

    Klimas, S.J.; Searle, H.; Stolberg, L.

    2010-01-01

    A capsule method has been developed and employed to measure the degradation rates of selected materials under some of the most challenging conditions relevant to the sulphur-iodine (SI) and the copper-chlorine (Cu-Cl) thermochemical cycles for hydrogen production. The materials tested so far include metals and engineering alloys, structural and functional polymers, elastomers, carbon-based materials, ceramics and glasses, and composites. A number of characterization methods have been used to detect and quantify the degradation of the diverse materials and, when feasible, establish the mode of attack. The paper details the results of this ongoing experimental investigation. The investigation currently focuses on the copper-chlorine hybrid cycle. The environment representative of the conditions in the electrolyser subsystem was approximated with an aqueous solution of hydrochloric acid (13.6 mol/kg), copper(II) chloride (1.36 mol/kg) and copper(I) chloride (1.36 mol/kg) at 160°C and 2.5 MPa (absolute). The current (tentative) recommendations for the selection of the materials required for the construction of the electrolyser subsystem of the copper-chlorine hybrid cycle, and the associated rationale, are presented and discussed. (author)

  7. The investigation of efficient conditions for alumina production from diasporic bauxites

    International Nuclear Information System (INIS)

    Alp, A.; Aydin, A.O.

    2002-01-01

    The main objective of this study was to investigate the leach conditions of diasporic bauxites in the region of Mugla-Milas for alumina production. The chemical composition was 57.91% Al 2 O 3 , 21.33% Fe 2 O 3 , 3.84% SiO 2 , 2.20% TiO 2 , 2.10% CaO, 1.02% FeS 2 , 1.65% CO 2 , and 0.52% other. The effects of alkaline (CaO and/or Na 2 CO 3 ) additives, precalcination temperature, calcination time, leaching time, solution concentration, liquid/bauxite ratio and particle size on the efficiency of alumina and silica dissolution were investigated. The optimum conditions of the process were the addition of alkaline in 0.5 mole fraction, 900 o C precalcination temperature, 2 hours of calcination time, 3 hours of leaching time, 1.5 molar Na 2 O of caustic concentration and liquid/bauxite ratio of 30 at the boiling temperature of leach solution under continuous stirring. Although the highest extraction efficiency of Al 2 O 3 was found to be 81.77% with precalcination at 1200 o C, 900 o C was preferred as an optimum calcination temperature. (author)

  8. Genetic relationship between body condition score, fertility, type and production traits in Brown Swiss dairy cows

    Directory of Open Access Journals (Sweden)

    Martino Cassandro

    2010-01-01

    Full Text Available This study aimed to investigate the relationship between body condition score (BCS, calving interval (CI, angularity (ANG, strength (FV and milk yield (MY on Brown Swiss cattle using data collected in the alpine provinces of Bolzano- Bozen and Trentino. The data set consisted of 28,538 test day records of BCS and MY from 3,282 Brown Swiss cows in lactation reared in 109 herds; production traits were merged with 13,796 repeated individual calving interval records and 38,711 type traits records. A multi-traits REML animal model was used to estimate (covariance components, with repeated observations. Heritability estimates for BCS, FV and ANG was 18%, 18% and 27%, respectively, while estimates for CI was very low (2%. Genetic correlations between CI and BCS was -0.44; between BCS and ANG was - 0.64; between BCS and MY was -0.35; between ANG and CI was 0.12. In conclusion, the selection for MY and ANG negatively affect fertility and average condition score of Brown cows. BCS recorded during lactation could be proposed as a useful trait for indirect selection aimed to improve fertility of cows.

  9. Water reactor fuel behaviour and fission products release in off-normal and accident conditions

    International Nuclear Information System (INIS)

    1987-09-01

    The present meeting was scheduled by the International Atomic Energy Agency upon the proposal of the Members of the International Working Group on Water Reactor Fuel Performance and Technology and held at the IAEA Headquarters in Vienna from 10 to 13 November 1986. Thirty participants from 17 countries and an international organization attended the meeting. Eighteen papers were presented from 13 countries and one international organization. The meeting was composed of four sessions and covered subjects related to: physico-chemical properties of core materials under off-normal conditions, and their interactions up to and after melt-down (5 papers); core materials deformation, relocation and core coolability under (severe) accident conditions (4 papers); fission products release: including experience, mechanisms and modelling (5 papers); power plant experience (4 papers). A separate abstract was prepared for each of these 18 papers. Four working groups covering the above-mentioned topics were held to discuss the present status of the knowledge and to develop recommendations for future activities in this field. Refs, figs and tabs

  10. Bio-hydrogen production from waste fermentation. Mixing and static conditions

    Energy Technology Data Exchange (ETDEWEB)

    Gomez, X.; Cuetos, M.J.; Prieto, J.I.; Moran, A. [Chemical Engineering Dept. IRENA, University of Leon, Avda. de Portugal 41, 24071 Leon (Spain)

    2009-04-15

    One of the main disadvantages of the dark fermentation process is the cost associated with the stages needed for obtaining H{sub 2} producing microorganisms. Using anaerobic microflora in fermentation systems directly is an alternative which is gaining special interest when considering the implementation of large-scale plants and the use of wastes as substrate material. The performance of two H{sub 2} producing microflora obtained from different anaerobic cultures was studied in this paper. Inoculum obtained from a waste sludge digester and from a laboratory digester treating slaughterhouse wastes were used to start up H{sub 2} fermentation systems. Inoculum acclimatized to slaughterhouse wastes gave better performance in terms of stability. However, due to the limited availability of this seed material, further work was performed to study the behaviour of the inoculum obtained from the municipal wastewater treatment plant. The process was evaluated under static and mixing conditions. It was found that application of a low organic loading rate favoured the performance of the fermentation systems, and that agitation of the reacting mass could alleviate unsteady performance. Specific H{sub 2} production obtained was in the range of 19-26 L/kg SV{sub fed} with maximum peak production of 38-67 L/kg SV{sub fed}. Although the performance of the systems was unsteady, recovery could be achieved by suspending the feeding process and controlling the pH in the range of 5.0-5.5. Testing the recovery capacity of the systems under temperature shocks resulted in total stoppage of H{sub 2} production. (author)

  11. Energy production from grassland - Assessing the sustainability of different process chains under German conditions

    International Nuclear Information System (INIS)

    Roesch, Christine; Skarka, J.; Raab, K.; Stelzer, V.

    2009-01-01

    In many regions of Europe, grassland shapes the landscape and fulfils important functions in protecting nature, soil, and water. However, the traditional uses of grassland for forage production are vanishing with progress in breeding and structural adaptations in agriculture. On the other hand, the demand for biomass energy is rising due to political sustainability goals and financial measures to support renewable energy. Against this background, the Institute for Technology Assessment and Systems Analysis investigated the applicability, economic efficiency, and sustainability of different techniques for energy production from grassland as well as from grassland converted into maize fields or short-rotation poplars under German conditions. The results show that despite relatively high energy prices and the financial support for bioenergy, the effects of energy production from grassland on employment in agriculture and farmers' income are modest. What is beneficial are savings in non-renewable energy, reductions in greenhouse gas emissions, and local provision of energy carriers. If grassland biomass (grass silage or hay) is used for energy purposes, this brings the further advantages of preserving biodiversity and the cultural landscape and protecting of soil and groundwater. Negative impacts on sustainable development result from an increase in emissions, which leads to acidification, eutrophication, and risks to human health. The overall evaluation indicates that short-rotation poplars are comparatively advantageous from the economic and ecological point of view. Therefore, a development plan for grassland is required to identify areas where grassland could be used as an energy resource or where it would be favourable to install energy plantations with fast-growing perennial plants

  12. Fission product releases at severe LWR accident conditions: ORNL/CEA measurements versus calculations

    Energy Technology Data Exchange (ETDEWEB)

    Andre, B.; Ducros, G.; Leveque, J.P. [CEA Centre d`Etudes de Grenoble, 38 (France). Dept. de Thermohydraulique et de Physique; Osborne, M.F.; Lorenz, R.A. [Oak Ridge National Lab., TN (United States); Maro, D. [CEA Centre d`Etudes de Fontenay-aux-Roses, 92 (France). Dept. de Protection de l`Environnement et des Installations

    1995-12-31

    Experimental programs in the United States and France have followed similar paths in supplying much of the data needed to analyze severe accidents. Both the HI/VI program, conducted at the Oak Ridge National Laboratory (ORNL) under the sponsorship of the U. S. Nuclear Regulatory Commission (NRC), and the HEVA/VERCORS program, supported by IPSN-Commissariat a l`Energie Atomique (CEA) and carried out at the Centre d`Etudes Nucleaires de Grenoble, have studied fission product release from light water reactor (LWR) fuel samples during test sequences representative of severe accidents. Recognizing that more accurate data, i.e., a better defined source term, could reduce the safety margins included in the rather conservative source terms originating from WASH-1400, the primary objective of these programs has been to improve the data base concerning fission product release and behavior at high temperatures. To facilitate the comparison, a model based on fission product diffusion mechanisms that was developed at ORNL and adapted with CEA experimental data is proposed. This CEA model is compared with the ORNL experimental data in a blind test. The two experimental programs used similar techniques in out-of-pile studies. Highly irradiated fuel samples were heated in radiofrequency induction furnaces to very high temperatures (up to 2700 K at ORNL and 2750 K at CEA) in oxidizing (H{sub 2}O), reducing (H{sub 2}) or mixed (H{sub 2}O+H{sub 2}) environments. The experimental parameters, which were chosen from calculated accident scenarios, did not duplicate specific accidents, but rather emphasized careful control of test conditions to facilitate extrapolation of the results to a wide variety of accident situations. This paper presents a broad and consistent database from ORNL and CEA release results obtained independently since the early 1980`S. A comparison of CORSOR and CORSOR Booth calculations, currently used in safety analysis, and the experimental results is presented and

  13. Furan Levels and Sensory Profiles of Commercial Coffee Products Under Various Handling Conditions.

    Science.gov (United States)

    Han, Jeesoo; Kim, Mina K; Lee, Kwang-Geun

    2017-11-01

    In this study, the levels of furan in coffee with consideration towards common coffee consumption was investigated. The concentration of furan in brewed coffee was the highest among the coffee types studied, with an average of 110.73 ng/mL, followed by canned coffee (28.08 ng/mL) and instant coffee (8.55 ng/mL). In instant and brewed coffee, the furan levels decreased by up to an average of 20% and 22%, after 5 min of pouring in a cup without a lid. The degree of reduction was greater when coffee was served without a lid, regardless of coffee type (P coffee, the level of furan decreased by an average of 14% after storage at 60 °C without a lid, and the degree of furan reduction in coffee was greater in coffee served warm (60 °C) than in coffee served cold (4 °C). A time-dependent intensities of sensory attributes in commercial coffees with various handling condition were different (P coffee kept in a cup with lid closed, holds the aroma of coffee longer than coffee in a cup without a lid. Consumption of coffee has increased rapidly in Korea over the past few years. Consequently, the probability of exposure to chemical hazards presence in coffee products increases. Furan is a heterocyclic compound, formed mainly from Maillard reaction, therefore present in coffee products. This work demonstrated the strategy to reduce the levels of furan in coffee products at individual consumer level, by investigating the levels of furan served in common handling scenarios of coffee in Korea: canned coffee, instant coffee, and brewed coffee. Findings of this study can practically guide industry, government, and consumer agencies to reduce the risk exposure to furan during coffee consumptions. © 2017 Institute of Food Technologists®.

  14. Removal of pharmaceutical and personal care products (PPCPs) under nitrifying and denitrifying conditions.

    Science.gov (United States)

    Suarez, Sonia; Lema, Juan M; Omil, Francisco

    2010-05-01

    The contribution of volatilization, sorption and transformation to the removal of 16 Pharmaceutical and Personal Care Products (PPCPs) in two lab-scale conventional activated sludge reactors, working under nitrifying (aerobic) and denitrifying (anoxic) conditions for more than 1.5 years, have been assessed. Pseudo-first order biological degradation rate constants (k(biol)) were calculated for the selected compounds in both reactors. Faster degradation kinetics were measured in the nitrifying reactor compared to the denitrifying system for the majority of PPCPs. Compounds could be classified according to their k(biol) into very highly (k(biol)>5Lg(SS)(-1)d(-1)), highly (1fragrances (HHCB, AHTN and ADBI) were transformed to a large extent under aerobic (>75%) and anoxic (>65%) conditions, whereas naproxen (NPX), ethinylestradiol (EE2), roxithromycin (ROX) and erythromycin (ERY) were only significantly transformed in the aerobic reactor (>80%). The anti-depressant citalopram (CTL) was moderately biotransformed under both, aerobic and anoxic conditions (>60% and >40%, respectively). Some compounds, as carbamazepine (CBZ), diazepam (DZP), sulfamethoxazole (SMX) and trimethoprim (TMP), manifested high resistance to biological transformation. Solids Retention Time (SRT(aerobic) >50d and 20d and <20d) had a slightly positive effect on the removal of FLX, NPX, CTL, EE2 and natural estrogens (increase in removal efficiencies <10%). Removal of diclofenac (DCF) in the aerobic reactor was positively affected by the development of nitrifying biomass and increased from 0% up to 74%. Similarly, efficient anoxic transformation of ibuprofen (75%) was observed after an adaptation period of 340d. Temperature (16-26 degrees C) only had a slight effect on the removal of CTL which increased in 4%.

  15. Near-extreme system condition and near-extreme remaining useful time for a group of products

    International Nuclear Information System (INIS)

    Wang, Hai-Kun; Li, Yan-Feng; Huang, Hong-Zhong; Jin, Tongdan

    2017-01-01

    When a group of identical products is operating in field, the aggregation of failures is a catastrophe to engineers and customers who strive to develop reliable and safe products. In order to avoid a swarm of failures in a short time, it is essential to measure the degree of dispersion from different failure times in a group of products to the first failure time. This phenomenon is relevant to the crowding of system conditions near the worst one among a group of products. The group size in this paper represents a finite number of products, instead of infinite number or a single product. We evaluate the reliability of the product fleet from two aspects. First, we define near-extreme system condition and near-extreme failure time for offline solutions, which means no online observations. Second, we apply them to a continuous degradation system that breaks down when it reaches a soft failure threshold. By using particle filtering in the framework of prognostics and health management for a group of products, we aim to estimate near-extreme system condition and further predict the remaining useful life (RUL) using online solutions. Numerical examples are provided to demonstrate the effectiveness of the proposed method. - Highlights: • The aggregation of failures is measured for a group of identical products. • The crowding of failures is quantitated by the near-extreme evaluations. • Near-extreme system condition are given for offline solutions. • Near-extreme remaining useful time are provided for online solutions.

  16. Safety aspects of electric energy production and supplies in conditions of the Slovenske elektrarne, a.s

    International Nuclear Information System (INIS)

    Sip, M.; Danilak, M. et al.

    2005-01-01

    In this presentation author deals with safety aspects of electric energy production and supplies in conditions of the Slovenske elektrarne, a.s. Some terrorist attack and accidents are presented. Four video-sequences are included

  17. Supplemental irrigation to improve wheat production and water use efficiency under rainfed farming conditions

    International Nuclear Information System (INIS)

    Hussain, Q.; Bhatti, A.A.; Ahmad, M.M.

    2007-01-01

    The stochastic behaviors of rainfall pose serious limitations for sustained and profitable crop production in rainfed areas; farmers hesitate to apply fertilizers when they are not sure about rainfall. In view of these limitations a research study was conducted for three years (2003-2006) at field station of Water Resources Research Institute (WRRI), National Agricultural Research Centre(NARC), Islamabad to examine the effects of supplemental irrigation (SI) on wheat production and water use efficiency (WUE). Irrigation treatments employed under the experiment were: i) Rainfed without irrigation and fertilizer application (I/sub 0/); ii) SI of 25 mm was applied to non-fertilizer field at 75% management allowed deficit (MAD)(I/sub 1/); iii) Rainfed with fertilizer application at sowing time (I/sub 2/); and iv) SI of 25 mm was applied at 75% MAD and at the time of fertilizer application as top dressing (I/sub 3/). Supplemental irrigation increased the crop yield during the years 2003-2006 under both fertilizer and non-fertilizer conditions. Increased in grain yield under non-fertilizer conditions (I/sub 1/) ranges between 770-980 kg/ha, which is 27 to 48% higher than the rainfed yield (I/sub 0/). Supplemental irrigation and split application of fertilizer (treatment I/sub 3/) increased in grain yield within the range of 1000-1350 kg/ha, which is 27-49% higher than yield under treatment I/sub 2/. Whereas, due to synergetic effect of supplemental irrigation and fertilizer application, increased in grain yield ranges between 1550-2030 kg/ha, which is 49% to 100% higher than the rainfed and non-fertilizer field. WUE was calculated for rain (WUE/sub r/) for total water (grass: previous soil water storage + rain + irrigation) (WUE/sub g/), for SI water only (WUE/sub si/) and for synergetic effect (SI water + fertilizer application) (WUE/sub sis/) Water use efficiencies namely the WUE/sub r/, WUE/sub g/ and WUE/sub si/ during the period of three years under non fertilizer

  18. The association between chronic conditions and non-agricultural work productivity loss among the middle-aged Chinese population.

    Science.gov (United States)

    Zhang, Wei; Sun, Huiying; Li, Xin

    2018-05-03

    To measure the association between different chronic conditions and non-agricultural work productivity loss among the middle-aged Chinese population. We used 2011, 2013 and 2015 data from the China Health and Retirement Longitudinal Study. The study focused on middle-aged respondents who had the potential to work in the non-agricultural sector. Work productivity loss was measured by non-agricultural work participation and number of absent workdays among those conducting non-agricultural work. Seven different chronic conditions were considered. Heart diseases had the strongest association with lower work participation for men and women. Stomach/digestive disease and arthritis/rheumatism were conditions with the largest incremental absent workdays for men and women, respectively. The associations with non-agricultural work productivity loss varied by chronic conditions, outcomes, and sex. The findings will help motivate chronic condition prevention/management programs and set priorities.

  19. Microalgae for high-value compounds and biofuels production: a review with focus on cultivation under stress conditions.

    Science.gov (United States)

    Markou, Giorgos; Nerantzis, Elias

    2013-12-01

    Microalgal biomass as feedstock for biofuel production is an attracting alternative to terrestrial plant utilization for biofuels production. However, today the microalgal cultivation systems for energy production purposes seem not yet to be economically feasible. Microalgae, though cultivated under stress conditions, such as nutrient starvation, high salinity, high temperature etc. accumulate considerable amounts (up to 60-65% of dry weight) of lipids or carbohydrates along with several secondary metabolites. Especially some of the latter are valuable compounds with an enormous range of industrial applications. The simultaneous production of lipids or carbohydrates for biofuel production and of secondary metabolites in a biorefinery concept might allow the microalgal production to be economically feasible. This paper aims to provide a review on the available literature about the cultivation of microalgae for the accumulation of high-value compounds along with lipids or carbohydrates focusing on stress cultivation conditions. © 2013.

  20. Alkaline conditions stimulate the production of 1,3-propanediol in Lactobacillus panis PM1 through shifting metabolic pathways.

    Science.gov (United States)

    Grahame, Douglas A S; Kang, Tae Sun; Khan, Nurul H; Tanaka, Takuji

    2013-07-01

    A novel Lactobacillus panis PM1 isolate was found to be capable of converting glycerol to 1,3-propanediol (1,3-PDO), an increasingly valuable commodity chemical. In this study the effects of various process parameters, including glucose and glycerol concentrations, inoculum size, temperature, aeration, pH, and carbon source were examined to determine the optimal conditions for the production of 1,3-PDO using a culture method simulating late log to early stationary phases. Inoculum size did not influence the production of 1,3-PDO, and temperature variance showed similar 1,3-PDO production between 25 and 37 °C under the examined conditions. Glycerol concentration and pH played a primary role in the final concentration of 1,3-PDO. The highest production occurred at 150-250 mM glycerol when 50 mM glucose was available. Alkaline initial conditions (pH 9-10) stimulated the production of 1,3-PDO which concurrently occurred with increased acetic acid production. Under these conditions, 213.6 mM of 1,3-PDO were produced from 300 mM glycerol (conversion efficiency was 71 %). These observations indicated that the production of 1,3-PDO was associated with the shift of the metabolic end-product ethanol to acetic acid, and that this shift resulted in an excess concentration of NADH available for the processing of glycerol to 1,3-PDO.

  1. 77 FR 12059 - Using Innovative Technologies and Other Conditions of Safe Use To Expand Which Drug Products Can...

    Science.gov (United States)

    2012-02-28

    ...] Using Innovative Technologies and Other Conditions of Safe Use To Expand Which Drug Products Can Be... over-the- counter or OTC products) can be purchased by consumers in pharmacies, supermarkets, and other retail establishments without the need for a prescription. Currently, consumers can purchase...

  2. "It's Not Like a Normal 9 to 5!": The Learning Journeys of Media Production Apprentices in Distributed Working Conditions

    Science.gov (United States)

    Lahiff, Ann; Guile, David

    2016-01-01

    An apprenticeship in media production in England is at the centre of this case study exploration. The context is exemplified by the organisation of the process of production around project teams and the development of project-based working cultures. Given these developments, the working conditions and learning opportunities presented to…

  3. Modelling of fission product release behavior from HTR spherical fuel elements under accident conditions

    International Nuclear Information System (INIS)

    Verfondern, K.; Mueller, D.

    1991-01-01

    Computer codes for modelling the fission product release behavior of spherical fuel elements for High Temperature Reactors (HTR) have been developed for the purpose of being used in risk analyses for HTRs. An important part of the validation and verification procedure for these calculation models is the theoretical investigation of accident simulation experiments which have been conducted in the KueFA test facility in the Hot Cells at KFA. The paper gives a presentation of the basic modeling and the calculational results of fission product release from modern German HTR fuel elements in the temperature range 1600-1800 deg. C using the TRISO coated particle failure model PANAMA and the diffusion model FRESCO. Measurements of the transient release behavior for cesium and strontium and of their concentration profiles after heating have provided informations about diffusion data in the important retention barriers of the fuel: silicon carbide and matrix graphite. It could be shown that the diffusion coefficients of both cesium and strontium in silicon carbide can significantly be reduced using a factor in the range of 0.02 - 0.15 compared to older HTR fuel. Also in the development of fuel element graphite, a tendency towards lower diffusion coefficients for both nuclides can be derived. Special heating tests focussing on the fission gases and iodine release from the matrix contamination have been evaluated to derive corresponding effective diffusion data for iodine in fuel element graphite which are more realistic than the iodine transport data used so far. Finally, a prediction of krypton and cesium release from spherical fuel elements under heating conditions will be given for fuel elements which at present are irradiated in the FRJ2, Juelich, and which are intended to be heated at 1600/1800 deg. C in the KueFA furnace in near future. (author). 7 refs, 11 figs

  4. Effect of basic alkali-pickling conditions on the production of lysinoalanine in preserved eggs.

    Science.gov (United States)

    Zhao, Yan; Luo, Xuying; Li, Jianke; Xu, Mingsheng; Tu, Yonggang

    2015-09-01

    During the pickling process, strong alkali causes significant lysinoalanine (LAL) formation in preserved eggs, which may reduce the nutritional value of the proteins and result in a potential hazard to human health. In this study, the impacts of the alkali treatment conditions on the production of LAL in preserved eggs were investigated. Preserved eggs were prepared using different times and temperatures, and alkali-pickling solutions with different types and concentrations of alkali and metal salts, and the corresponding LAL contents were measured. The results showed the following: during the pickling period of the preserved egg, the content of LAL in the egg white first rapidly increased and then slowly increased; the content of LAL in the egg yolk continued to increase significantly. During the aging period, the levels of LAL in both egg white and egg yolk slowly increased. The amounts of LAL in the preserved eggs were not significantly different at temperatures between 20 and 25ºC. At higher pickling temperatures, the LAL content in the preserved eggs increased. With the increase of alkali concentration in the alkali-pickling solution, the LAL content in the egg white and egg yolk showed an overall trend of an initial increase followed by a slight decrease. The content of LAL produced in preserved eggs treated with KOH was lower than in those treated with NaOH. NaCl and KCl produced no significant effects on the production of LAL in the preserved eggs. With increasing amounts of heavy metal salts, the LAL content in the preserved eggs first decreased and then increased. The LAL content generated in the CuSO4 group was lower than that in either the ZnSO4 or PbO groups. © 2015 Poultry Science Association Inc.

  5. Thermodynamic assessment of hydrogen production and cobalt oxidation susceptibility under ethanol reforming conditions

    International Nuclear Information System (INIS)

    Avila, C.N. de; Hori, C.E.; Assis, A.J. de

    2011-01-01

    A comparative thermodynamic analysis of ethanol reforming reactions was conducted using an in-house code. Equilibrium compositions were estimated using the Lagrange multipliers method, which generated systems of non-linear algebraic equations, solved numerically. Effects of temperature, pressure and steam to ethanol, O 2 to ethanol and CO 2 to ethanol ratios on the equilibrium compositions were evaluated. The validation was done by comparing these data with experimental literature. The results of this work proved to be useful to foresee whether the experimental results follow the stoichiometry of the reactions involved in each process. Mole fractions of H 2 and CO 2 proved to be the most reliable variables to make this type of validation. Maximization of H 2 mole fraction was attained between 773 and 873 K, but maximum net mole production of H 2 was only achieved at higher temperatures (>1123 K). This work also advances in the thermodynamics of solid-gas phase interactions. A solid phase thermodynamic analysis was performed to confirm that Co 0 formation from CoO is spontaneous under steam reforming conditions. The results showed that this reduction process occurs only for temperatures higher than 430 K. It was also found that once reduced, Co based catalysts will never oxidize back to Co 3 O 4 . -- Highlights: → Thermodynamic analysis of ethanol reforming reactions using an in-house code. → Analysis performed by solving systems of non-linear algebraic equations. → H 2 and CO 2 equilibrium data are useful to validate catalytic tests. → Maximization of H 2 mole fraction achieved between 773 and 873 K → CoO reduction is spontaneous under steam reforming of ethanol conditions.

  6. The Eocene Arctic Azolla bloom: environmental conditions, productivity and carbon drawdown.

    Science.gov (United States)

    Speelman, E N; Van Kempen, M M L; Barke, J; Brinkhuis, H; Reichart, G J; Smolders, A J P; Roelofs, J G M; Sangiorgi, F; de Leeuw, J W; Lotter, A F; Sinninghe Damsté, J S

    2009-03-01

    Enormous quantities of the free-floating freshwater fern Azolla grew and reproduced in situ in the Arctic Ocean during the middle Eocene, as was demonstrated by microscopic analysis of microlaminated sediments recovered from the Lomonosov Ridge during Integrated Ocean Drilling Program (IODP) Expedition 302. The timing of the Azolla phase (approximately 48.5 Ma) coincides with the earliest signs of onset of the transition from a greenhouse towards the modern icehouse Earth. The sustained growth of Azolla, currently ranking among the fastest growing plants on Earth, in a major anoxic oceanic basin may have contributed to decreasing atmospheric pCO2 levels via burial of Azolla-derived organic matter. The consequences of these enormous Azolla blooms for regional and global nutrient and carbon cycles are still largely unknown. Cultivation experiments have been set up to investigate the influence of elevated pCO2 on Azolla growth, showing a marked increase in Azolla productivity under elevated (760 and 1910 ppm) pCO2 conditions. The combined results of organic carbon, sulphur, nitrogen content and 15N and 13C measurements of sediments from the Azolla interval illustrate the potential contribution of nitrogen fixation in a euxinic stratified Eocene Arctic. Flux calculations were used to quantitatively reconstruct the potential storage of carbon (0.9-3.5 10(18) gC) in the Arctic during the Azolla interval. It is estimated that storing 0.9 10(18) to 3.5 10(18) g carbon would result in a 55 to 470 ppm drawdown of pCO2 under Eocene conditions, indicating that the Arctic Azolla blooms may have had a significant effect on global atmospheric pCO2 levels through enhanced burial of organic matter.

  7. Prevalence of gastrointestinal helminth infections in free-range laying hens under mountain farming production conditions.

    Science.gov (United States)

    Wuthijaree, K; Lambertz, C; Gauly, M

    2017-12-01

    1. A cross-sectional study was conducted from September 2015 to July 2016 in South Tyrol, Northern Italy to examine the prevalence of gastrointestinal helminths in free-range laying hens under mountain farming production conditions. 2. A total of 280 laying hens from 14 free-range mountain farms (4 organic, 10 conventional) were randomly collected at the end of the laying period. Faecal samples were taken to analyse faecal egg counts (FEC) and faecal oocyst counts (FOC). The gastrointestinal tracts were removed post mortem and examined for the presence of helminths. 3. In faeces, FEC values averaged 258 eggs per g of faeces, which were dominated by Ascaridia galli and Heterakis gallinarum. Mean FOC was 80 oocysts/g. In the gastrointestinal tract, at least one nematode species was found in 99.3% of the examined hens. H. gallinarum was the most prevalent nematode (95.7%), followed by Capillaria spp. (66.8%) and A. galli (63.6%). Thirty per cent of the chickens were infected with cestodes (tapeworms). Correlation coefficients between worm counts of H. gallinarum, Capillaria spp. and A. galli ranged from 0.41 to 0.51. 5. The helminth prevalence did not differ between conventional and organic farms, whereas total worm burden was higher in organic compared with conventional farms (318.9 vs. 112.0). Prevalence and infection intensity did not differ between farms that used anthelmintic treatments and those that did not. 6. In conclusion, free-range laying hens under the studied mountain farming conditions are at high risk of nematode infection, especially in organic systems. The vast majority of hens are subclinical infected with at least one helminth species.

  8. Improving the productive and reproductive efficiency in the new zealand rabbits under the egyptian conditions

    International Nuclear Information System (INIS)

    El-Tarabany, A.A.E.I.

    2008-01-01

    The present work was planned to study the effects of different alleviation techniques under both mild and hot conditions on productive and reproductive traits of growing female and male rabbits, mature bucks and mature does during pregnancy. In growing males and females, the results showed that the heat stress conditions caused significant decrease in body weight, daily body gain, feed consumption, as well as, significant decrease in most blood hematology, blood components and blood minerals, blood vitamins and hormonal levels, and increase in water intake, concentration of creatinine. In mature bucks, heat stress caused significant decrease in physical and chemical of semen characteristics and blood components. In pregnant does, heat stress caused significant decrease in DOE traits, as well as, significant decrease in most blood hematology, blood components and blood minerals, blood vitamins and hormonal levels, and increase in water intake.With regard to the effects of alleviation of heat load by drinking cool water during hot period or by injection of Tri-B two times weakly for growing male and female rabbits, as compared to without treated rabbits caused significant increase in growth performance, physiological traits and economic traits. In mature bucks, short treatment by injection of Tri-B induced increase in semen quality, i.e., ejaculate volume, sperm motility and sperm-cell concentration and decrease in dead sperm, sperm abnormalities and acrosomal abnormalities. In pregnant does, treatment by cool water during hot period improved daily body gain, litter size, litter weight, feed consumption and blood components and significant decrease in percentage of mortality rate.

  9. Determination of optimal conditions of oxytetracyclin production from streptomyces rimosus; Optimisation de la production de l'oxytetracycline par Streptomyces rimosus

    Energy Technology Data Exchange (ETDEWEB)

    Zouaghi, Atef [Institut National des Sciences Appliquees et de Technologie (Tunisia)

    2007-07-01

    Streptomyces rimosus is an oxytetracycline (OTC) antibiotic producing bacteria that exhibited activities against gram positive and negative bacteria. OTC is used widely not only in medicine but also in production industry. The antibiotic production of streptomyces covers a very wide range of condition. However, antibiotic producers are particularly fastidious cultivated by proper selection of media such as carbon source. In present study we have optimised conditions of OTC production (Composition of production media, p H, shaking and temperature). The results have been shown that bran barley is the optimal media for OTC production at 28C pH5.8 at 150rpm for 5 days. For antibiotic determination, OTC was extracted with different organic solvent. Thin-layer chromatography system was used for separation and identification of OTC antibiotic. High performance liquid chromatographic (HPLC) method with ultraviolet detection for the analysis of OTC is applied to the determination of OTC purification. (Author). 24 refs.

  10. Effects of preservation conditions of canine feces on in vitro gas production kinetics and fermentation end-products

    NARCIS (Netherlands)

    Bosch, G.; Wrigglesworth, D.J.; Cone, J.W.; Pellikaan, W.F.; Hendriks, W.H.

    2013-01-01

    This study investigated the effect of chilling and freezing (for 24 h) canine feces on in vitro gas production kinetics and fermentation end-product profiles from carbohydrate-rich (in vitro run 1) and protein-rich substrates (in vitro run 2). Feces were collected from 3 adult Retriever-type dogs

  11. PRODUCTIVE AND REPRODUCTIVE PERFORMANCE AND THEIR INTERACTION IN CROSSBRED CATTLE UNDER FIELD CONDITIONS IN DISTRICT BANNU

    Directory of Open Access Journals (Sweden)

    M. Suhban Qureshi, Asmatullah Khan1, Khuda Bakhsh Mirbaharland M. Oris Samol

    2000-01-01

    Full Text Available A study was conducted in district Bannu located in the southern dry piedmont mountains of the NWF Province of Pakistan. Records of 300 crossbred cows, produced through use of Holstein Friesian semen in native, non-discript cows, were studies. Data on various productive and reproductive parameters were analyzed. The average age at puberty was 752 ±10.9 days, ranging from 420 to 1620 days and was positively correlated with mounting (r = 0. 14 and negatively with estrus duration, vaginal mucus and calving interval (r = -0.14, -0.13, -0.15, respectively; P<0.05. The average inter-estrus interval was 20.0 ± 9.07 days, ranging from 15 to 24 days. Estrus duration was 34.0 ± 0.85 hours, ranging from 17 to 72 hours and was positively correlated with vaginal mucus (r = 0.12 and services per conception (r = 0.14 and negative with bellowing (r = 0. 11, P< 0.05 number of services per conception in the crossbred cows averaged 1.6+0.1 ranging from 1 to 12 and was correlated positively, with daily milk yield and negatively with lactation length and vaginal mucus (r = 0.32. -0.31, -0.27 respectively, P< 0.01. calving interval was 612 ± 4.56 days, ranging from 360 to 900 days and was negatively correlated with age at puberty, daily milk yield and use of oxytocin (r = -0.15. -0.67, -0.62, respectively, P < 0.0. The average lactation length of the cows was 503 ± 6.36 days, ranging from 30 to 1441 days. The mean daily milk yield was 10.1 ± 0.14 Kg ranging from 1.0 to18.0 Kg. The standard deviation (SD for average daily milk yield was 2.36 Kg. Number of animals 1 SD above mean was 28.0% and those 2 SD above mean was 17.0%. Number of animals 1 SD below mean was 51.4% and those 2 SD below mean was 3.6%. The wide variation makes a good base for productivity enhancement through selective breeding. It may be concluded that productive and reproductive performance is satisfactory in crossbred cattle under field conditions showing their adaptability to the local

  12. Dietary fat affects heat production and other variables of equine performance, under hot and humid conditions.

    Science.gov (United States)

    Kronfeld, D S

    1996-07-01

    Does dietary fat supplementation during conditioning improve athletic performance, especially in the heat? Fat adaptation has been used to increase energy density, decrease bowel bulk and faecal output and reduce health risks associated with hydrolysable carbohydrate overload. It may also reduce spontaneous activity and reactivity (excitability), increase fatty acid oxidation, reduce CO2 production and associated acidosis, enhance metabolic regulation of glycolysis, improve both aerobic and anaerobic performance and substantially reduce heat production. A thermochemical analysis of ATP generation showed the least heat release during the direct oxidation of long chain fatty acids, which have a 3% advantage over glucose and 20 to 30% over short chain fatty acids and amino acids. Indirect oxidation via storage as triglyceride increased heat loss during ATP generation by 3% for stearic acid, 65% for glucose and 174% for acetic acid. Meal feeding and nutrient storage, therefore, accentuates the advantage of dietary fat. A calorimetric model was based on initial estimates of net energy for competitive work (10.76 MJ for the Endurance Test of an Olympic level 3-day-event), other work (14.4 MJ/day) and maintenance (36 MJ), then applied estimates of efficiencies to derive associated heat productions for the utilisation of 3 diets, Diet A: hay (100), Diet B: hay and oats (50:50) and Diet C: hay, oats and vegetable oil (45:45:10), the difference between the last 2 diets representing fat adaptation. During a 90.5 min speed and stamina test, heat production was estimated as 37, 35.4 and 34.6 MJ for the 3 diets, respectively, an advantage 0.8 MJ less heat load for the fat adapted horse, which would reduce water needed for evaporation by 0.33 kg and reduce body temperature increase by about 0.07 degree C. Total estimated daily heat production was 105, 93 and 88 MJ for the 3 diets, respectively, suggesting a 5 MJ advantage for the fat adapted horse (Diet C vs. Diet B). Estimated

  13. Optimization of fermentation conditions for 1,3-propanediol production by marine Klebsiella pneumonia HSL4 using response surface methodology

    Science.gov (United States)

    Li, Lili; Zhou, Sheng; Ji, Huasong; Gao, Ren; Qin, Qiwei

    2014-09-01

    The industrially important organic compound 1,3-propanediol (1,3-PDO) is mainly used as a building block for the production of various polymers. In the present study, response surface methodology protocol was followed to determine and optimize fermentation conditions for the maximum production of 1,3-PDO using marine-derived Klebsiella pneumoniae HSL4. Four nutritional supplements together with three independent culture conditions were optimized as follows: 29.3 g/L glycerol, 8.0 g/L K2 HPO4, 7.6 g/L (NH4)2 SO4, 3.0 g/L KH2 PO4, pH 7.1, cultivation at 35°C for 12 h. Under the optimal conditions, a maximum 1,3-PDO concentration of 14.5 g/L, a productivity of 1.21 g/(L·h) and a conversion of glycerol of 0.49 g/g were obtained. In comparison with the control conditions, fermentation under the optimized conditions achieved an increase of 38.8% in 1,3-PDO concentration, 39.0% in productivity and 25.7% in glycerol conversion in flask. This enhancement trend was further confirmed when the fermentation was conducted in a 5-L fermentor. The optimized fermentation conditions could be an important basis for developing lowcost, large-scale methods for industrial production of 1,3-PDO in the future.

  14. Metal complexation in near field conditions of nuclear waste repository - stability constant of copper complexation with cellulose degradation products, in alkaline conditions

    International Nuclear Information System (INIS)

    Guede, Kipre Bertin

    2005-11-01

    Copper is a stable element and spent fuel component which constitutes the radioactive waste. The reaction of Copper with cellulose degradation products in alkaline conditions was performed to mimic what occurs in near field conditions of nuclear waste repository. From the characteristics of Cu (II), this thesis aims at inferring the behaviour of radionuclides vis a vis the degradation products of cellulose. The contribution of the present work is therefore the assessment of the stability of the major cellulose degradation product, its affinity for Copper and the extent of the complexation function 13 between Cu (II) and the organic moieties. The formation of cellulose degradation products was followed by measurement of p11, Conductivity, Angle of rotation, relative abundance of aliphatics and aromatics (E4/E6 ) aid by UV-visible spectroscopy. The TOC was determined using the Walkley and Black titration after respectively 31 weeks and 13 weeks of degradation for the reaction mixtures T and A, N. The stability of the major degradation products gave the following figures: ISA(A): - 13 43.39 <ΔG -10639.88 ISA(N): - Ii 436.45<ΔG< -9103.6. The study of the characteristics of Gluconic Acid, as a model compound, was carried out in an attempt to give a general picture of the roper ties of cellulose degradation products. The Complexation between Cu (II) and the organic ligand (Cellulose degradation products) was performed using UV-visible spectroscopy and Ion Distribution technique. The Log B value obtained from the complexation studies at 336 nm for 1 = 0. I Ni NaClO4 and I = 0.01 M NaClO4, falls within a range of 3.48 to 3.74 for the standard reference material (Gluconic Acid), and within I .87 to 2.3 I, and I .6 to 2.01, respectively for the degradation Products ISA (A) and ISA(N). The ion distribution studies showed that: • In (he absence of the degradation product ISA and at pH = 3.68. 56. 17 % of Cu (II) was bound to the resin. • In the presence of ISA and at 2

  15. Hygienic effects and gas production of plastic bio-digesters under tropical conditions.

    Science.gov (United States)

    Yen-Phi, Vo Thi; Clemens, Joachim; Rechenburg, Andrea; Vinneras, Björn; Lenssen, Christina; Kistemann, Thomas

    2009-12-01

    Plastic plug-flow bio-digesters have been promoted as a good option for improved treatment of manure and wastewater in developing countries although minimal information has been published on their hygienic status. This bench-scale study replicates bio-digester conditions to evaluate the reduction of pathogen and indicator microorganisms at three different hydraulic retention times (HRT) in the anaerobic treatment of pig manures at 30 degrees C for 50 days. Results showed that physicochemical values differed between HRTs. Gas production efficiency was better for longer HRTS. The accumulated sludge at the reactor's base increased with longer HRT. Phages and bacteria examined were reduced, but none was completely eliminated. Log10 reduction of bacteria ranged from 0.54 to 2.47. Phages ranged from 1.60 to 3.42. The reduction of organisms at HRT = 30 days was about one log10 unit higher than HRT = 15 days and about two log10 units higher than HRT = 3 days. The results indicate that the reduction of tested organisms increases with HRT. However the hygienic quality of the liquid effluent does not meet required quality values for surface and irrigation water. Longer HRTs are recommended to increase gas yield and achieve higher pathogen reduction. More barriers should be applied while handling bio-digester outputs to minimise risks to environmental and human health.

  16. Growth, Toxin Production and Allelopathic Effects of Pseudo-nitzschia multiseries under Iron-Enriched Conditions

    Directory of Open Access Journals (Sweden)

    Bruna Fernanda Sobrinho

    2017-10-01

    Full Text Available In order to assess the effects of Fe-enrichment on the growth and domoic acid (DA production of the toxigenic diatom Pseudo-nitzschia multiseries, static cultures that received the addition of different iron (Fe concentrations were maintained for 30 days. Intra- and extracellular DA concentrations were evaluated over time, and growth and chain-formation were compared to those of non-toxic diatoms, Bacillaria sp. Growth rates of P. multiseries (μ = 0.45–0.73 d−1 were similar among cultures containing different Fe concentrations. Likewise, the similar incidence and length of P. multiseries stepped cell chains (usually 2–4; up to 8-cell long among the treatments reinforces that the cultures were not growth-inhibited under any condition tested, suggesting an efficient Fe acquisition mechanism. Moreover, DA concentrations were significantly higher under the highest Fe concentration, indicating that Fe is required for toxin synthesis. Bacillaria sp. reached comparable growth rates under the same Fe concentrations, except when the dissolved cell contents from a P. multiseries culture was added. The 50–70% reduction in cell density and 70–90% decrease in total chlorophyll-a content of Bacillaria sp. at early stationary growth phase indicates, for the first time, an allelopathic effect of undetermined compounds released by Pseudo-nitzschia to another diatom species.

  17. Evaluation of some soil amendments plant productivity under saline conditions using nuclear techniques

    International Nuclear Information System (INIS)

    Aly, E.A.K.

    2004-01-01

    this study was carried out in Wadi Ras Sudr (south Saini government). this location was characterized as poor soil with high salinity (wasteland). in the same time it suffers from shortage of water resources. therefore, we aimed to utilize this soil as well as the saline ground water for introducing it into production systems. the reclamation of virgin poor soil need large efforts and much research, especially plant exposure to salinity which is rapidly followed by a decrease in growth rate. the use of natural organic sources as organic fertilizers improve the growth and yields of plants, and safe the environment from pollution . organic fertilizers (Of) such as green manure (G M) or poultry manure (P M) can be used as nutrient sources for good plant growth, where the soil amendments improve the physical, chemical and biological properties of the soil. economically, the yield improvement and nutrient supply will reflect the potential use of such organic materials. also , phosphorus and/or potassium supplementation separately or in combination with O F (G M and/or P M) improved the growth of both barley and wheat plants under such adverse condition of salinity using 15 N isotope dilution technique

  18. Effect of climatic conditions and irrigation on sugarbeet production in the Vojvodina Province, Serbia and Montenegro

    International Nuclear Information System (INIS)

    Maksimovic, Livija; Dragovic, Svetimir

    2004-01-01

    The climatic conditions in the Vojvodina Province, the main agricultural region of Serbia and Montenegro, are generally favorable for crop production, but the rainfall during growing season is frequently too low or irregular to secure high and stable yields of agricultural crops including sugarbeet. In climatic conditions where there is an uneven amount and distribution of precipitation, as is the case with this region, sugarbeet yield performance is directly dependent on weather conditions. Sugarbeet yields vary not only from one year to another but also from one region to another within the single growing season. The average sugar beet water requirement for the period 1987-2002 was 586 mm, with fairly small variations by either the region or the year. About 10-20% of the total sugarbeet requirement for water comes from reserve soil water, while the rest is provided by rainfall and irrigation. The actual evapotranspiration is 392 mm on the average, ranging from 198 mm in dry years to 542 mm in rainy years. The average precipitation sum for the growing season at Rimski Sancevi site was 360 mm, with high annual variation - from 138 to 683 mm. The average water deficit relative to the sugar beet water requirement was 190 mm, ranging from 24 to 367 mm. The deficit was especially pronounced in July and August, when it ranged between 0 and 282 mm, averaging 146 mm, or 75% of the total requirement. Sugarbeet root yield is directly proportional to the amount of rainfall during growing season and the amount of available water spent for actual evapotranspiration. Relatively high yields have been obtained in years with high amounts of rainfall (from 500 to 650 mm) and favorable distribution of rainfall during growing season. High yields were also obtained in years with relatively low amounts of rainfall but moderately favorable or favorable distribution of rainfall. In the period 1990-1999, root yields in commercial production varied significantly, from the average of 22.7 t

  19. Modelling of Ethanol Production from Red Beet Juice by Saccharomyces cerevisiae under Thermal and Acid Stress Conditions

    Directory of Open Access Journals (Sweden)

    Donaji Jiménez-Islas

    2014-01-01

    Full Text Available In this work the effects of pH and temperature on ethanol production from red beet juice by the strains Saccharomyces cerevisiae ITD00196 and S. cerevisiae ATCC 9763 are studied. Logistic, Pirt, and Luedeking-Piret equations were used to describe quantitatively the microbial growth, substrate consumption, and ethanol production, respectively. The two S. cerevisiae strains used in this study were able to produce ethanol with high yield and volumetric productivity under acid and thermal stress conditions. The equations used to model the fermentation kinetics fit very well with the experimental data, thus establishing that ethanol production was growth associated under the evaluated conditions. The yeast S. cerevisiae ITD00196 had the best fermentative capacity and could be considered as an interesting option to develop bioprocesses for ethanol production.

  20. Life cycle assessment of biofuel production from brown seaweed in Nordic conditions

    DEFF Research Database (Denmark)

    Alvarado-Morales, Merlin; Boldrin, Alessio; Karakashev, Dimitar Borisov

    2013-01-01

    the sustainability of the process. Two scenarios were analyzed for the brownseaweed (Laminaria digitata), namely, biogas production (scenario 1) and bioethanol + biogas production (scenario 2). Potential environmental impact categories under investigation were Global Warming, Acidification and Terrestrial...

  1. Stability and oxidation products of hydrolysable tannins in basic conditions detected by HPLC/DAD-ESI/QTOF/MS.

    Science.gov (United States)

    Tuominen, Anu; Sundman, Terhi

    2013-01-01

    Hydrolysable tannins occur in plants that are used for food or medicine by humans or herbivores. Basic conditions can alter the structures of tannins, that is, the oxidation of phenolic groups can lead to the formation of toxic quinones. Previously, these labile quinones and other oxidation products have been studied with colorimetric or electron paramagnetic resonance methods, which give limited information about products. To study the stability and oxidation products of hydrolysable tannins in basic conditions using HPLC with a diode-array detector (DAD) combined with electrospray ionisation (ESI) and quadrupole time-of-flight (QTOF) MS. Three galloyl glucoses, four galloyl derivatives with different polyols and three ellagitannins were purified from plants. The incubation reactions of tannins were monitored by HPLC/DAD at five pH values and in reduced oxygen conditions. Reaction products were identified based on UV spectra and mass spectral fragmentation obtained with the high-resolution HPLC/DAD-ESI/QTOF/MS. The use of a base-resistant HPLC column enabled injections without the sample pre-treatment and thus detection of short-lived products. Hydrolysable tannins were unstable in basic conditions and half-lives were mostly less than 10 min at pH 10. Degradation rates were faster at pH 11 but slower at milder pH. The HPLC analyses revealed that various products were formed and identified to be the result of hydrolysis, deprotonation and oxidation. Interestingly, the main hydrolysis product was ellagic acid; it was also formed from galloyl glucoses that do not contain oxidatively coupled galloyl groups in their initial structures. HPLD/DAD-ESI/QTOF/MS was an efficient method for the identification of polyphenol oxidation products and showed how different pH conditions determine the fate of hydrolysable tannins. Copyright © 2013 John Wiley & Sons, Ltd.

  2. Consolidated bioprocessing strategy for ethanol production from Jerusalem artichoke tubers by Kluyveromyces marxianus under high gravity conditions.

    Science.gov (United States)

    Yuan, W J; Chang, B L; Ren, J G; Liu, J P; Bai, F W; Li, Y Y

    2012-01-01

    Developing an innovative process for ethanol fermentation from Jerusalem artichoke tubers under very high gravity (VHG) conditions. A consolidated bioprocessing (CBP) strategy that integrated inulinase production, saccharification of inulin contained in Jerusalem artichoke tubers and ethanol production from sugars released from inulin by the enzyme was developed with the inulinase-producing yeast Kluyveromyces marxianus Y179 and fed-batch operation. The impact of inoculum age, aeration, the supplementation of pectinase and nutrients on the ethanol fermentation performance of the CBP system was studied. Although inulinase activities increased with the extension of the seed incubation time, its contribution to ethanol production was negligible because vigorously growing yeast cells harvested earlier carried out ethanol fermentation more efficiently. Thus, the overnight incubation that has been practised in ethanol production from starch-based feedstocks is recommended. Aeration facilitated the fermentation process, but compromised ethanol yield because of the negative Crabtree effect of the species, and increases the risk of contamination under industrial conditions. Therefore, nonaeration conditions are preferred for the CBP system. Pectinase supplementation reduced viscosity of the fermentation broth and improved ethanol production performance, particularly under high gravity conditions, but the enzyme cost should be carefully balanced. Medium optimization was performed, and ethanol concentration as high as 94·2 g l(-1) was achieved when 0·15 g l(-1) K(2) HPO(4) was supplemented, which presents a significant progress in ethanol production from Jerusalem artichoke tubers. A CBP system using K. marxianus is suitable for efficient ethanol production from Jerusalem artichoke tubers under VHG conditions. Jerusalem artichoke tubers are an alternative to grain-based feedstocks for ethanol production. The high ethanol concentration achieved using K. marxianus with the

  3. Design Features of Product-Integrated PV : An Evaluation of Various Factors under Indoor Irradiance Conditions

    NARCIS (Netherlands)

    Apostolou, G.

    2016-01-01

    This thesis explores the field of product-integrated photovoltaics (PIPV), a term which is used for all types of products that contain solar cells in one or more of their surfaces, aiming at providing power during the product’s use. Product-integrated photovoltaics (PIPV) began to be widely

  4. Improving yield and water productivity of maize grown under deficit-irrigated in dry area conditions

    Directory of Open Access Journals (Sweden)

    Mohamed H. Abd el-wahed

    2015-10-01

    Full Text Available Scarcity of water is the most severe constraint for development of maize in arid and semi-arid areas. Based on the actual crop need, the irrigation management has to be improved so that the water supply to the crop can be reduced while still achieving high yield. Therefore, the current study has been organized to evaluate the effects of deficit sprinkler irrigation (DSI and farmyard manure (FYM on Grain yield (GY and crop water productivity (CWP of corn, a 2-year experiment was conducted in arid region of Libya. The DSI treatments were (I100 = 100%, I85 = 85% or I70 = 70% of the crop evapotranspiration. FYM treatments were (0, 10 ton ha−1 spread either on the soil surface, incorporated with surface or subsurface layer (FYM10s, FYM10m or FYM10ss, respectively and 20 ton ha−1 spread as before (FYM20s, FYM20m or FYM20ss, respectively. Results indicated that the highest values of grain yield (GY were obtained from I100 treatment, while the lowest were observed in I70. FYM20ss enhanced GY than other FYM treatments in both seasons. The highest GY and CWP were recorded with I100 and received FYM20ss. It could be considered as a suitable under arid environmental conditions and similar regions, the treatment (I100 × FYM20ss is the most suitable for producing high GY and CWP. Under limited irrigation water, application of (I85 ×FYM20ss treatment was found to be favorable to save 15% of the applied irrigation water, at the time in which produced the same GY.

  5. Light Conditions Affect the Measurement of Oceanic Bacterial Production via Leucine Uptake

    Science.gov (United States)

    Morán, Xosé Anxelu G.; Massana, Ramon; Gasol, Josep M.

    2001-01-01

    The effect of irradiance in the range of 400 to 700 nm or photosynthetically active radiation (PAR) on bacterial heterotrophic production estimated by the incorporation of 3H-leucine (referred to herein as Leu) was investigated in the northwestern Mediterranean Sea and in a coastal North Atlantic site, with Leu uptake rates ranging over 3 orders of magnitude. We performed in situ incubations under natural irradiance levels of Mediterranean samples taken from five depths around solar noon and compared them to incubations in the dark. In two of the three stations large differences were found between light and dark uptake rates for the surfacemost samples, with dark values being on average 133 and 109% higher than in situ ones. Data obtained in coastal North Atlantic waters confirmed that dark enclosure may increase Leu uptake rates more than threefold. To explain these differences, on-board experiments of Leu uptake versus irradiance were performed with Mediterranean samples from depths of 5 and 40 m. Incubations under a gradient of 12 to 1,731 μmol of photons m−2 s−1 evidenced a significant increase in incorporation rates with increasing PAR in most of the experiments, with dark-incubated samples departing from this pattern. These results were not attributed to inhibition of Leu uptake in the light but to enhanced bacterial response when transferred to dark conditions. The ratio of dark to light uptake rates increased as dissolved inorganic nitrogen concentrations decreased, suggesting that bacterial nutrient deficiency was overcome by some process occurring only in the dark bottles. PMID:11525969

  6. Cultivating conditions effects on kefiran production by the mixed culture of lactic acid bacteria imbedded within kefir grains.

    Science.gov (United States)

    Zajšek, Katja; Goršek, Andreja; Kolar, Mitja

    2013-08-15

    The influence of fermentation temperature, agitation rate, and additions of carbon sources, nitrogen sources, vitamins and minerals on production of kefiran by kefir grains lactic acid bacteria was studied in a series of experiments. The main aim of the work was to increase the exopolysaccharide (EPS) production where customised milk was used as fermentation medium. It was proved that the controlling of culturing conditions and the modifying of fermentation medium conditions (i.e., carbon, nitrogen, mineral sources and vitamins) can dramatically enhance the production of the EPS. The temperature and agitation rate were critical for kefiran production during the 24 h cultivation of grains; our optimised conditions being 25°C and 80 rpm, respectively. In addition, when optimising the effects of additional nutrition, it was found that 5% (w/v) lactose, 0.1% (w/v) thiamine, and 0.1% (w/v) FeCl3 led to the maximal production of EPS. The results indicate that nutrients can be utilised to improve the production of EPS and that good kefir grains growth does not appear to be a determining factor for a high production yield of EPS. Copyright © 2013 Elsevier Ltd. All rights reserved.

  7. Experiments on Nitrogen Oxide Production of Droplet Arrays Burning under Microgravity Conditions

    Science.gov (United States)

    Moesl, Klaus; Sattelmayer, Thomas; Kikuchi, Masao; Yamamoto, Shin; Yoda, Shinichi

    The optimization of the combustion process is top priority in current aero-engine and aircraft development, particularly from the perspectives of high efficiency, minimized fuel consumption, and a sustainable exhaust gas production. Aero-engines are exclusively liquid-fueled with a strong correlation between the combustion temperature and the emissions of nitric oxide (NOX ). Due to safety concerns, the progress in NOX reduction has been much slower than in stationary gas turbines. In the past, the mixing intensity in the primary zone of aero-engine combustors was improved and air staging implemented. An important question for future aero-engine combustors, consequently, is how partial vaporization influences the NOX emissions of spray flames? In order to address this question, the combustion of partially vaporized, linear droplet arrays was studied experimentally under microgravity conditions. The influence of fuel pre-vaporization on the NOX emissions was assessed in a wide range. The experiments were performed in a drop tower and a sounding rocket campaign. The microgravity environment provided ideal experiment conditions without the disturbing ef-fect of natural convection. This allowed the study of the interacting phenomena of multi-phase flow, thermodynamics, and chemical kinetics. This way the understanding of the physical and chemical processes related to droplet and spray combustion could be improved. The Bremen drop tower (ZARM) was utilized for the precursor campaign in July 2008, which was com-prised of 30 drops. The sounding rocket experiments, which totaled a microgravity duration of 6 minutes, were finally performed on the flight of TEXUS-46 in November 2009. On both campaigns the "Japanese Combustion Module" (JCM) was used. It is a cooperative experi-ment on droplet array combustion between the Japan Aerospace Exploration Agency (JAXA) and ESA's (European Space Agency) research team, working on the combustion properties of partially premixed sprays

  8. Behaviour of steel corrosion products under neutral-oxidizing water conditions

    International Nuclear Information System (INIS)

    Martynova, O.I.; Petrova, T.I.; Samojlov, Yu.F.; Kharitonova, N.L.

    1985-01-01

    Results of laboratory experiments on studying the solubility of iron and cobalt corrosion products are given. It is established that oxygen dosage doesn't influence practically on the iron corrosion product solubility but cobalt corrosion product solubility decreases, the presence of hydrogen peroxide in an initial solution leads to increase of the iron corrosion product solubility especially at 125 deg C. It is shown that hydrogen peroxide affects unambiguously the cobalt corrosion product solubility: at hydrogen peroxide concentration of about 400 μg/l at 50-275 deg C temperature their solubility is minimum

  9. Design Features of Product-Integrated PV: An Evaluation of Various Factors under Indoor Irradiance Conditions

    OpenAIRE

    Apostolou, G.

    2016-01-01

    This thesis explores the field of product-integrated photovoltaics (PIPV), a term which is used for all types of products that contain solar cells in one or more of their surfaces, aiming at providing power during the product’s use. Product-integrated photovoltaics (PIPV) began to be widely introduced around 2000, although the use of PV systems in products dates back to the 70s. PIPV includes products such as PV-powered boats, aircrafts, cars, bicycles, camping tents, street lights, recycling...

  10. OPTIMIZATION OF THE CULTURE CONDITIONS FOR TANNASE PRODUCTION BY Aspergillus sp. GM4 UNDER SOLID-STATE FERMENTATION

    Directory of Open Access Journals (Sweden)

    Patrícia Nirlane da Costa Souza

    2015-03-01

    Full Text Available The production of tannase by Aspergillus sp. GM4 under solid-state fermentation (SSF  was investigated using different vegetables leaves such as mango, jamun, coffee and agricultural residues such as coffee husks, rice husks and wheat bran. Among substrates used jamun leaves yielded high tannase production. The Plackett-Burman design was conducted to evaluate the effects of 12 independent variables on the production of tannase under SSF using jamun leaves as substrate. Among these variables, incubation time, potassium nitrate and tannic acid had significant effects on enzyme production. The best incubation time was studied and others variables were optimized using the Central Composite Design. The best conditions for tannase production were: incubation time of 2 days; tannic acid 1.53% (w/w and potassium nitrate 2.71% (w/w. After the optimization process, the tannase production increased 4.65-fold. Keywords: surface response methodology; enzyme; jamun

  11. Trends, Productivity Losses, and Associated Medical Conditions Among Toxoplasmosis Deaths in the United States, 2000–2010

    Science.gov (United States)

    Cummings, Patricia L.; Kuo, Tony; Javanbakht, Marjan; Sorvillo, Frank

    2014-01-01

    Few studies have quantified toxoplasmosis mortality, associated medical conditions, and productivity losses in the United States. We examined national multiple cause of death data and estimated productivity losses caused by toxoplasmosis during 2000–2010. A matched case–control analysis examined associations between comorbid medical conditions and toxoplasmosis deaths. In total, 789 toxoplasmosis deaths were identified during the 11-year study period. Blacks and Hispanics had the highest toxoplasmosis mortality compared with whites. Several medical conditions were associated with toxoplasmosis deaths, including human immunodeficiency virus (HIV), lymphoma, leukemia, and connective tissue disorders. The number of toxoplasmosis deaths with an HIV codiagnosis declined from 2000 to 2010; the numbers without such a codiagnosis remained static. Cumulative disease-related productivity losses for the 11-year period were nearly $815 million. Although toxoplasmosis mortality has declined in the last decade, the infection remains costly and is an important cause of preventable death among non-HIV subgroups. PMID:25200264

  12. Optimization of Large-Scale Culture Conditions for the Production of Cordycepin with Cordyceps militaris by Liquid Static Culture

    Directory of Open Access Journals (Sweden)

    Chao Kang

    2014-01-01

    Full Text Available Cordycepin is one of the most important bioactive compounds produced by species of Cordyceps sensu lato, but it is hard to produce large amounts of this substance in industrial production. In this work, single factor design, Plackett-Burman design, and central composite design were employed to establish the key factors and identify optimal culture conditions which improved cordycepin production. Using these culture conditions, a maximum production of cordycepin was 2008.48 mg/L for 700 mL working volume in the 1000 mL glass jars and total content of cordycepin reached 1405.94 mg/bottle. This method provides an effective way for increasing the cordycepin production at a large scale. The strategies used in this study could have a wide application in other fermentation processes.

  13. The Influence Of Highway Transportation Infrastructure Condition Toward Commodity Production Generation for The Resilience Needs at Regional Internal Zone

    Science.gov (United States)

    Akbardin, Juang; Parikesit, Danang; Riyanto, Bambang; Mulyono, Agus Taufik

    2018-02-01

    The poultry commodity consumption and requirement is one of the main commodities that must be fulfilled in a region to maintain the availability of meat from poultry. Poultry commodity production is one of the production sectors that have a clean environment resistance. An increasing of poultry commodity generation production requires a smooth distribution to arrive at the processing. The livestock location as a commodity production is placed at a considerable far distance from residential and market locations. Zones that have poultry commodity production have an excess potential to supply other zones that are lacking in production to the consumption of these commodities. The condition of highway transportation infrastructure that is very diverse with the damage level availability in a zone has an influence in the supply and demand of poultry commodity requirement in the regional internal of Central Java province. In order to know the effect of highway transportation infrastructure condition toward the poultry commodity movement, demography factor and availability of freight vehicles will be reviewed to estimate the amount of poultry commodity movement generation production. Thus the poultry commodity consumption requirement that located in the internal - regional zone of central java province can be adequated from the zone. So it can be minimized the negative impacts that affect the environment at the zone in terms of comparison of the movement attraction and generation production at poultry commodity in Central Java.

  14. Nitrate reductase and nitrous oxide production by Fusarium oxysporum 11dn1 under aerobic and anaerobic conditions.

    Science.gov (United States)

    Kurakov, A V; Nosikov, A N; Skrynnikova, E V; L'vov, N P

    2000-08-01

    The fungus Fusarium oxysporum 11dn1 was found to be able to grow and produce nitrous oxide on nitrate-containing medium in anaerobic conditions. The rate of nitrous oxide formation was three to six orders of magnitude lower than the rates of molecular nitrogen production by common denitrifying bacteria. Acetylene and ammonia did not affect the release of nitrous oxide release. It was shown that under anaerobic conditions fast increase of nitrate reductase activity occurred, caused by the synthesis of enzyme de novo and protein dephosphorylation. Reverse transfer of the mycelium to aerobic conditions led to a decline in nitrate reductase activity and stopped nitrous oxide production. The presence of two nitrate reductases was shown, which differed in molecular mass, location, temperature optima, and activity in nitrate- and ammonium-containing media. Two enzymes represent assimilatory and dissimilatory nitrate reductases, which are active in aerobic and anaerobic conditions, respectively.

  15. Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase

    Directory of Open Access Journals (Sweden)

    Biplab Kumar Dash

    2015-01-01

    Full Text Available A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp. and identified as Bacillus subtilis BI19. The effect of various fermentation conditions on amylase production through shake-flask culture was investigated. Rice flour (1.25% as a cheap natural carbon source was found to induce amylase production mostly. A combination of peptone and tryptone as organic and ammonium sulfate as inorganic nitrogen sources gave highest yield. Maximum production was obtained after 24 h of incubation at 37°C with an initial medium pH 8.0. Addition of surfactants like Tween 80 (0.25 g/L and sodium lauryl sulfate (0.2 g/L resulted in 28% and 15% increase in enzyme production, respectively. Amylase production was 3.06 times higher when optimized production conditions were used. Optimum reaction temperature and pH for crude amylase activity were 50°C and 6.0, respectively. The crude enzyme showed activity and stability over a fair range of temperature and pH. These results suggest that B. subtilis BI19 could be exploited for production of amylase at relatively low cost and time.

  16. Molecular Identification of a Newly Isolated Bacillus subtilis BI19 and Optimization of Production Conditions for Enhanced Production of Extracellular Amylase.

    Science.gov (United States)

    Dash, Biplab Kumar; Rahman, M Mizanur; Sarker, Palash Kumar

    2015-01-01

    A study was carried out with a newly isolated bacterial strain yielding extracellular amylase. The phylogenetic tree constructed on the basis of 16S rDNA gene sequences revealed this strain as clustered with the closest members of Bacillus sp. and identified as Bacillus subtilis BI19. The effect of various fermentation conditions on amylase production through shake-flask culture was investigated. Rice flour (1.25%) as a cheap natural carbon source was found to induce amylase production mostly. A combination of peptone and tryptone as organic and ammonium sulfate as inorganic nitrogen sources gave highest yield. Maximum production was obtained after 24 h of incubation at 37 °C with an initial medium pH 8.0. Addition of surfactants like Tween 80 (0.25 g/L) and sodium lauryl sulfate (0.2 g/L) resulted in 28% and 15% increase in enzyme production, respectively. Amylase production was 3.06 times higher when optimized production conditions were used. Optimum reaction temperature and pH for crude amylase activity were 50 °C and 6.0, respectively. The crude enzyme showed activity and stability over a fair range of temperature and pH. These results suggest that B. subtilis BI19 could be exploited for production of amylase at relatively low cost and time.

  17. Cellulase production by Trichoderma harzianum in static and mixed solid-state fermentation reactors under nonaseptic conditions

    Energy Technology Data Exchange (ETDEWEB)

    Deschamps, F.; Giuliano, C.; Asther, M.; Huet, M.C.; Roussos, S.

    1985-09-01

    Cellulase production from lignocellulosic materials was studied in solid-state cultivation by both static and mixed techniques under nonaseptic conditions. The effects of fermentation conditions, such as moisture content, pH, temperature, and aeration, on cellulase production by Trichoderma harzianum using a mixture of wheat straw (80%) and bran (20%) were investigated. With a moisture content of 74% and a pH of 5.8, 18 IU filter paper activity and 198 IU endoglucanase activity/g initial substrate content were obtained in 66 hours. The extension from static column cultivation to stirred tank reactor of 65 l capacity gave similar yields of cellulase.

  18. Aid conditionalities, international Good Manufacturing Practice standards and local production rights: a case study of local production in Nepal.

    Science.gov (United States)

    Brhlikova, Petra; Harper, Ian; Subedi, Madhusudan; Bhattarai, Samita; Rawal, Nabin; Pollock, Allyson M

    2015-06-14

    Local pharmaceutical production has been endorsed by the WHO as a means of addressing health priorities of developing countries. However, local producers of essential medicines must comply with international pharmaceutical standards in order to be eligible to compete in donor tenders. These standards determine production rights for on-patent and off-patent medicines, and guide international procurement of medicines. We reviewed the literature on the impact of Good Manufacturing Practice (GMP) on local production; a gap analysis from the literature review indicated a need for further research. Over sixty interviews were conducted with people involved in the Nepali pharmaceutical production and distribution chain from 2006 to 2009 on the GMP areas of relevance: regulatory capacity, staffing, funding and training, resourcing of GMP, inspectors' interpretation of the rules and compliance. Although Nepal producers have increased their overall share of the domestic market, only the public manufacturer, Royal Drugs, focuses on medicines for public health programmes; private producers engage mainly in brand competition for private markets, not essential medicines. Nepali regulators and producers state that implementation of GMP standards is hindered by low regulatory capacity, insufficient training of staff in the industry, financial constraints and lack of investment for upgrading capital. The transition period to mandatory compliance with WHO GMP rules is lengthy. Less than half of private producers had WHO GMP in 2013. Producers are not directly affected by international harmonisation of standards as they do not export medicines and the Nepali regulator does not enforce the WHO standards strictly. Without an international GMP certificate they cannot tender for donor dependent health programmes. In Nepal, local private manufacturers focus mainly on brand competition for private consumption not essential medicines, the government preferentially procures essential

  19. Influence of the mycelium growth conditions on the production of amylolytic, proteolytic and pectinolytic enzymes by Aspergillus niger C

    Energy Technology Data Exchange (ETDEWEB)

    Fiedurek, J.; Ilczuk, Z.; Lobarzewski, J. (Uniwersytet Marii Curie-Sklodowskiej, Lublin (Poland). Inst. Mikrobiologii i Biochemii)

    1989-01-01

    Various nitrogen and carbon sources, as well as natural products, were examined as inducers of the production of amylases, proteases and pectinases by A. niger C. A. niger C grown on wheat bran extract medium provided culture supernatants with the highest enzymatic activities. Some culture conditions, e.g. pH, medium temperature and time period of cultivation, were optimalized to improve the growth and enzyme biosynthesis by A. niger C. (orig.).

  20. A 10-days heatwave around flowering superimposed on climate change conditions significantly affects production of 22 barley accessions

    DEFF Research Database (Denmark)

    Ingvordsen, Cathrine Heinz; Lyngkjær, Michael F.; Peltonen-Sainio, Pirjo

    2015-01-01

    Extreme climate events as heatwaves, floods and storms cause acute changes in season variability influencing primary production and are very likely to increase in magnitude and/or frequency (IPCC, AR5, WGI). In the present study 22 primarily Nordic barley accessions were grown in four basic clima...... climate change conditions on numerous accessions in order to select appropriate genotypes for breeding future cultivars that can secure the primary production....

  1. Molasses wastewater treatment and lipid production at low temperature conditions by a microalgal mutant Scenedesmus sp. Z-4

    OpenAIRE

    Ma, Chao; Wen, Hanquan; Xing, Defeng; Pei, Xuanyuan; Zhu, Jiani; Ren, Nanqi; Liu, Bingfeng

    2017-01-01

    Background Simultaneous wastewater treatment and lipid production by oleaginous microalgae show great potential to alleviate energy shortage and environmental pollution, because they exhibit tremendous advantages over traditional activated sludge. Currently, most research on wastewater treatment by microalgal are carried out at optimized temperature conditions (25?35??C), but no information about simultaneous wastewater treatment and lipid production by microalgae at low temperatures has been...

  2. The 1998 bible. Cold production, air conditioning, big cooking installations; La bible 1998. Froid, climatisation, grandes cuisines

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-12-31

    This manual is devoted to French professionals of thermal engineering. It contains all useful addresses of companies involved in the manufacturing, distribution and maintenance of air conditioning and refrigerating systems, a list of companies classified by products and activities, a departmental list of distribution companies, an alphabetical list of professional organizations, a catalog of the new products of the year, and the recent regulatory texts concerning the confinement of refrigerating installations. (J.S.)

  3. Effect of redox conditions on bacterial and fungal biomass and carbon dioxide production in Louisiana coastal swamp forest sediment

    International Nuclear Information System (INIS)

    Seo, Dong Cheol; DeLaune, Ronald D.

    2010-01-01

    Fungal and bacterial carbon dioxide (CO 2 ) production/emission was determined under a range of redox conditions in sediment from a Louisiana swamp forest used for wastewater treatment. Sediment was incubated in microcosms at 6 Eh levels (-200, -100, 0, + 100, + 250 and + 400 mV) covering the anaerobic range found in wetland soil and sediment. Carbon dioxide production was determined by the substrate-induced respiration (SIR) inhibition method. Cycloheximide (C 15 H 23 NO 4 ) was used as the fungal inhibitor and streptomycin (C 21 H 39 N 7 O 12 ) as the bacterial inhibitor. Under moderately reducing conditions (Eh > + 250 mV), fungi contributed more than bacteria to the CO 2 production. Under highly reducing conditions (Eh ≤ 0 mV), bacteria contributed more than fungi to the total CO 2 production. The fungi/bacteria (F/B) ratios varied between 0.71-1.16 for microbial biomass C, and 0.54-0.94 for microbial biomass N. Under moderately reducing conditions (Eh ≥ + 100 mV), the F/B ratios for microbial biomass C and N were higher than that for highly reducing conditions (Eh ≤ 0 mV). In moderately reducing conditions (Eh ≥ + 100 mV), the C/N microbial biomass ratio for fungi (C/N: 13.54-14.26) was slightly higher than for bacteria (C/N: 9.61-12.07). Under highly reducing redox conditions (Eh ≤ 0 mV), the C/N microbial biomass ratio for fungi (C/N: 10.79-12.41) was higher than for bacteria (C/N: 8.21-9.14). For bacteria and fungi, the C/N microbial biomass ratios under moderately reducing conditions were higher than that in highly reducing conditions. Fungal CO 2 production from swamp forest could be of greater ecological significance under moderately reducing sediment conditions contributing to the greenhouse effect (GHE) and the global warming potential (GWP). However, increases in coastal submergence associated with global sea level rise and resultant decrease in sediment redox potential from increased flooding would likely shift CO 2 production to bacteria

  4. Production of polyhydroxybutyrate and alginate from glycerol by Azotobacter vinelandii under nitrogen-free conditions

    OpenAIRE

    Yoneyama, Fuminori; Yamamoto, Mayumi; Hashimoto, Wataru; Murata, Kousaku

    2015-01-01

    Glycerol is an interesting feedstock for biomaterials such as biofuels and bioplastics because of its abundance as a by-product during biodiesel production. Here we demonstrate glycerol metabolism in the nitrogen-fixing species Azotobacter vinelandii through metabolomics and nitrogen-free bacterial production of biopolymers, such as poly-d-3-hydroxybutyrate (PHB) and alginate, from glycerol. Glycerol-3-phosphate was accumulated in A. vinelandii cells grown on glycerol to the exponential phase...

  5. Optimization of culture conditions for tannase production by Aspergillus sp. gm4 in solid state fermentation

    OpenAIRE

    Souza, Patrícia Nirlane da Costa; Universidade Federal de Lavras; Maia, Natália da Costa; Universidade Federal de Lavras; Guimarães, Luís Henrique Souza; Universidade de São Paulo; Resende, Mário Lúcio Vilela de; Universidade Federal de Lavras; Cardoso, Patrícia Gomes; Universidade Federal de Lavras

    2015-01-01

    The production of tannase by Aspergillus sp. GM4 under solid-state fermentation (SSF)  was investigated using different vegetables leaves such as mango, jamun, coffee and agricultural residues such as coffee husks, rice husks and wheat bran. Among substrates used jamun leaves yielded high tannase production. The Plackett-Burman design was conducted to evaluate the effects of 12 independent variables on the production of tannase under SSF using jamun leaves as substrate. Among these variables,...

  6. Convexity Conditions and the Legendre-Fenchel Transform for the Product of Finitely Many Positive Definite Quadratic Forms

    International Nuclear Information System (INIS)

    Zhao Yunbin

    2010-01-01

    While the product of finitely many convex functions has been investigated in the field of global optimization, some fundamental issues such as the convexity condition and the Legendre-Fenchel transform for the product function remain unresolved. Focusing on quadratic forms, this paper is aimed at addressing the question: When is the product of finitely many positive definite quadratic forms convex, and what is the Legendre-Fenchel transform for it? First, we show that the convexity of the product is determined intrinsically by the condition number of so-called 'scaled matrices' associated with quadratic forms involved. The main result claims that if the condition number of these scaled matrices are bounded above by an explicit constant (which depends only on the number of quadratic forms involved), then the product function is convex. Second, we prove that the Legendre-Fenchel transform for the product of positive definite quadratic forms can be expressed, and the computation of the transform amounts to finding the solution to a system of equations (or equally, finding a Brouwer's fixed point of a mapping) with a special structure. Thus, a broader question than the open 'Question 11' in Hiriart-Urruty (SIAM Rev. 49, 225-273, 2007) is addressed in this paper.

  7. Management of Brackish water for crop production under arid and semi-arid conditions

    International Nuclear Information System (INIS)

    Murtaza, G.; Ghafoor, A.; Akhtar, S.; Shah, S.H.; Mahmood, N.

    2005-01-01

    For sustainable crop production, changing soil or water chemistry so as to counter the adverse effects of brackish water is a good option. This is normally accomplished by soil or water applied amendments such as gypsum. The other option of blending or cycling brackish and non-brackish water also has merits to reduce the potential hazards. The biological and organic amendments improve soil physical conditions which, otherwise, are expected to be deteriorated by the use of brackish water. Keeping this in view, a field experiment was conducted on a non saline-non sodic sandy loam soil (EC/sub e/ 1.31-1.76 dS m/sup -1/, pH = 8.47-8.61, SAR = 5.50-7.41, infiltration rate 0.6-0.8 cm/h, bulk density = 1.56-1.61 Mg m/sup -3/ for the upper 15 cm soil depth) to evaluate the growth response of cotton crop to different soil and water treatments. Treatments included: T/sub 1/ canal water), T/sub 2/ [tube well water (EC = 3.38 dS m/sup -1/, SAR = 16.43 and RSC = 5.57 mmol/sub c/ L/sup -1/)], T3 [cyclic use (alternate irrigations with canal and tube well waters)], T/sub 4/ (tube well water as such + FYM at the rate of 25 Mg ha/sup -1/annually) and T/sub 5/ (tube well water + gypsum at the rate of water gypsum requirement (WRSC to be decreased up to 00). During the first year of experimentation seed cotton yield was not significantly affected by the applied treatments and was in the decreasing order of: T/sub 3/ (2361 kg ha/sup -1/) > T/sub 4/ (2073 kg ha/sup -1/) > T 1 (2015 kg ha/sup -1/) > T/sub 5/ (2001 kg ha.1 and T 2 (1982 ha/sup -1/. Number of bolls picked per plant was in the decreasing order of: T 2 (33) > T/sub 4/ (32) > T/sub 1/ (31) > T/sub 3/ (30) and T/sub 5/ (26) with non-significant treatment differences. The pH, EC/sub e/ and SAR values remained below safe limits by this cotton (first) crop. (author)

  8. Evaluation of Costs in Asparagus Production in Relation to Different Technological Processes in Conditions of Slovak Republic

    Directory of Open Access Journals (Sweden)

    Patrik Burg

    2014-01-01

    Full Text Available This study deals with analysis structure of cost on the production of asparagus in relation to three different technological processes in conditions of Slovak Republic. Obtained results can be used as a template to prepare budgets and make production decisions to estimate potential returns and to analyze investment and financial analysis decisions in the asparagus cultivation. Production practices used in the budget are based on typical practices for asparagus in this country. From the analysis of the cost structure of assessed variants different technological procedures follows that the largest share of the costs fall to purchased material and labor.

  9. Modelling and optimization of environmental conditions for kefiran production by Lactobacillus kefiranofaciens.

    Science.gov (United States)

    Cheirsilp, B; Shimizu, H; Shioya, S

    2001-12-01

    A mathematical model for kefiran production by Lactobacillus kefiranofaciens was established, in which the effects of pH, substrate and product on cell growth, exopolysaccharide formation and substrate assimilation were considered. The model gave a good representation both of the formation of exopolysaccharides (which are not only attached to cells but also released into the medium) and of the time courses of the production of galactose and glucose in the medium (which are produced and consumed by the cells). Since pH and both lactose and lactic acid concentrations differently affected production and growth activity, the model included the effects of pH and the concentrations of lactose and lactic acid. Based on the mathematical model, an optimal pH profile for the maximum production of kefiran in batch culture was obtained. In this study, a simplified optimization method was developed, in which the optimal pH profile was determined at a particular final fermentation time. This was based on the principle that, at a certain time, switching from the maximum specific growth rate to the critical one (which yields the maximum specific production rate) results in maximum production. Maximum kefiran production was obtained, which was 20% higher than that obtained in the constant-pH control fermentation. A genetic algorithm (GA) was also applied to obtain the optimal pH profile; and it was found that practically the same solution was obtained using the GA.

  10. Best Practices for New Product Pricing: Impact on Market Performance and Price Level under Different Conditions

    NARCIS (Netherlands)

    Ingenbleek, P.T.M.; Frambach, R.T.; Verhallen, T.M.M.

    2013-01-01

    To date, research on new product pricing has predominantly been approached as a choice between market skimming and penetration pricing. Despite calls for research that addresses other complexities in new product pricing, empirical research responding to these calls remains scarce. This paper

  11. Reactor internals production under ideal conditions at Pensacola. [PWR type reactors

    Energy Technology Data Exchange (ETDEWEB)

    1975-12-01

    The Westinghouse factory at Pensacola, Florida, which specialises in the production of pressure vessel internal components for PWRs, is described. Its excellent manufacturing and inspection facilities, supported by careful attention to staff training and motivation, are responsible for the extremely high level of quality and continual improvement in productivity.

  12. Effects of Mesophilic and Thermophilic Temperature Condition to Biogas Production (Methane from Palm Oil Mill Effluent (POME with Cow Manures

    Directory of Open Access Journals (Sweden)

    Muhammad Fajar Fajar

    2018-01-01

    Full Text Available Biogas is an environmentally friendly renewable energy source. Biogas can be used using Palm Oil Mill Effluents (POME. However, the % yield of biogas productivity is still not optimum due to the low conversion. The biogas productivity can be optimized by adding methanogen bacteria which increase the methane production through the anaerobic fermentation process. This study aims to utilize cow manures as the source of methanogen bacteria in methane production from POME. Furthermore, this study specifically aims to obtain the optimum productivity condition of biogas production by the composition ratio of POME and cow manures to the amount of fermentation time at 35oC and 50oC for mesophilic and thermophilic bacteria, respectively. The ratio of POME and cow mature were A1 (100:0, A2 (80:20, A3 (70:30, A4 (60:40, and A5 (0:100. The highest yield of biogas production was A2 ratio using the thermophilic condition which showed 51.33% mol with the total solid decline of 73.43%, COD removal of 77.01%, and BOD removal of 70.02%.

  13. Effect of bioconversion conditions on vanillin production by Amycolatopsis sp. ATCC 39116 through an analysis of competing by-product formation.

    Science.gov (United States)

    Ma, Xiao-kui; Daugulis, Andrew J

    2014-05-01

    This study investigated the effects of transformation conditions such as initial pH, the initial concentration of glucose and yeast extract in the medium, and the separate addition of ferulic acid and vanillic acid, on the production of vanillin through an analysis of competing by-product formation by Amycolatopsis sp. ATCC 39116. The extent and nature of by-product formation and vanillin yield were affected by initial pH and different initial concentrations of glucose and yeast extract in the medium, with a high yield of vanillin and high cell density obtained at pH 8.0, 10 g/l glucose, and 8 g/l yeast extract. High concentrations of ferulic acid were found to negatively affect cell density. Additional supplementation of 100 mg/l vanillic acid, a metabolically linked by-product, was found to result in a high concentration of vanillin and guaiacol, an intermediate of vanillin. Via an analysis of the effect of these transformation conditions on competing by-product formation, high concentrations of ferulic acid were transformed with a molar yield to vanillin of 96.1 and 95.2 %, by Amycolatopsis sp. ATCC 39116 and Streptomyces V1, respectively, together with a minor accumulation of by-products. These are among the highest performance values reported in the literature to date for Streptomyces in batch cultures.

  14. Joint optimization of condition-based maintenance and production lot-sizing

    NARCIS (Netherlands)

    Peng, H.; van Houtum, G.J.J.A.N.

    2016-01-01

    Due to the development of sensor technologies nowadays, condition-based maintenance (CBM) programs can be established and optimized based on the data collected through condition monitoring. The CBM activities can significantly increase the uptime of a machine. However, they should be conducted in a

  15. An expert fault diagnosis system for vehicle air conditioning product development

    NARCIS (Netherlands)

    Tan, C.F.; Tee, B.T.; Khalil, S.N.; Chen, W.; Rauterberg, G.W.M.

    2015-01-01

    The paper describes the development of the vehicle air-conditioning fault diagnosis system in automotive industries with expert system shell. The main aim of the research is to diagnose the problem of new vehicle air-conditioning system development process and select the most suitable solution to

  16. Patient preferences in the treatment of hemophilia A: impact of storage conditions on product choice.

    Science.gov (United States)

    Tischer, Bernd; Marino, Renato; Napolitano, Mariasanta

    2018-01-01

    To gain insights into the usage of factor VIII (FVIII) products by patients diagnosed with moderate/severe hemophilia A, and to assess the impact and perceived importance of product storage. In this study, 200 patients diagnosed with moderate or severe hemophilia A across seven countries participated. Data were collected via a 30-minute, face-to-face interview in six countries and via a web-based survey in the seventh country. The questionnaire evaluated the effect of six features associated with FVIII products on the choice of the product; the structure and flow of data collection was designed to eliminate potential bias. Two-thirds of the respondents were using recombinant FVIII products. Only 17% were generally dissatisfied with current FVIII products, whereas >40% of the respondents were dissatisfied with frequency of administration and storage issues when traveling. The majority noted restrictions in their daily activities, particularly travel and sports. Most of them (85%), stored their product in the refrigerator and of these, 88% believed that it should always be stored there. These patients were also less satisfied with the product overall, more concerned about storage temperature, more restricted in daily activities, and spent more time on preparation and injection compared with patients who stored their product at room temperature. Conjoint analysis revealed that origin of FVIII (plasma-derived vs recombinant) was the strongest driver of product choice among all respondents, followed by storage flexibility (temperature), reconstitution device, and administration frequency. In this study, we did not investigate the efficacy and safety of the product. Not refrigerating FVIII products was associated with greater patient satisfaction and less restriction on daily activities. If efficacy and safety are unaffected, then storing FVIII at room temperature might have a positive impact on product choice. Few patients were aware that FVIII can be stored without

  17. Patient preferences in the treatment of hemophilia A: impact of storage conditions on product choice

    Directory of Open Access Journals (Sweden)

    Tischer B

    2018-03-01

    Full Text Available Bernd Tischer,1 Renato Marino,2 Mariasanta Napolitano3 1Kantar Health, Munich, Germany; 2Haemophilia and Thrombosis Centre, University Hospital of Bari, Apulia, Italy; 3University of Palermo, Reference Regional Center for Thrombosis and Hemostasis Hematology Unit, Palermo, Italy Objectives: To gain insights into the usage of factor VIII (FVIII products by patients diagnosed with moderate/severe hemophilia A, and to assess the impact and perceived importance of product storage.Methods: In this study, 200 patients diagnosed with moderate or severe hemophilia A across seven countries participated. Data were collected via a 30-minute, face-to-face interview in six countries and via a web-based survey in the seventh country. The questionnaire evaluated the effect of six features associated with FVIII products on the choice of the product; the structure and flow of data collection was designed to eliminate potential bias.Results: Two-thirds of the respondents were using recombinant FVIII products. Only 17% were generally dissatisfied with current FVIII products, whereas >40% of the respondents were dissatisfied with frequency of administration and storage issues when traveling. The majority noted restrictions in their daily activities, particularly travel and sports. Most of them (85%, stored their product in the refrigerator and of these, 88% believed that it should always be stored there. These patients were also less satisfied with the product overall, more concerned about storage temperature, more restricted in daily activities, and spent more time on preparation and injection compared with patients who stored their product at room temperature. Conjoint analysis revealed that origin of FVIII (plasma-derived vs recombinant was the strongest driver of product choice among all respondents, followed by storage flexibility (temperature, reconstitution device, and administration frequency. In this study, we did not investigate the efficacy and safety of

  18. Identifying conditions for inducible protein production in E. coli: combining a fed-batch and multiple induction approach

    Directory of Open Access Journals (Sweden)

    Choi Young J

    2006-08-01

    Full Text Available Abstract Background In the interest of generating large amounts of recombinant protein, inducible systems have been studied to maximize both the growth of the culture and the production of foreign proteins. Even though thermo-inducible systems were developed in the late 1970's, the number of studies that focus on strategies for the implementation at bioreactor scale is limited. In this work, the bacteriophage lambda PL promoter is once again investigated as an inducible element but for the production of green fluorescent protein (GFP. Culture temperature, induction point, induction duration and number of inductions were considered as factors to maximize GFP production in a 20-L bioreactor. Results It was found that cultures carried out at 37°C resulted in a growth-associated production of GFP without the need of an induction at 42°C. Specific production was similar to what was achieved when separating the growth and production phases. Shake flask cultures were used to screen for desirable operating conditions. It was found that multiple inductions increased the production of GFP. Induction decreased the growth rate and substrate yield coefficients; therefore, two time domains (before and after induction having different kinetic parameters were created to fit a model to the data collected. Conclusion Based on two batch runs and the simulation of culture dynamics, a pre-defined feeding and induction strategy was developed to increase the volumetric yield of a temperature regulated expression system and was successfully implemented in a 20-L bioreactor. An overall cell density of 5.95 g DW l-1 was achieved without detriment to the cell specific production of GFP; however, the production of GFP was underestimated in the simulations due to a significant contribution of non-growth associated product formation under limiting nutrient conditions.

  19. Effect of weather data aggregation on regional crop simulation for different crops, production conditions, and response variables

    NARCIS (Netherlands)

    Zhao, Gang; Hoffmann, Holger; Bussel, Van L.G.J.; Enders, Andreas; Specka, Xenia; Sosa, Carmen; Yeluripati, Jagadeesh; Tao, Fulu; Constantin, Julie; Raynal, Helene; Teixeira, Edmar; Grosz, Balázs; Doro, Luca; Zhao, Zhigan; Nendel, Claas; Kiese, Ralf; Eckersten, Henrik; Haas, Edwin; Vanuytrecht, Eline; Wang, Enli; Kuhnert, Matthias; Trombi, Giacomo; Moriondo, Marco; Bindi, Marco; Lewan, Elisabet; Bach, Michaela; Kersebaum, Kurt Christian; Rötter, Reimund; Roggero, Pier Paolo; Wallach, Daniel; Cammarano, Davide; Asseng, Senthold; Krauss, Gunther; Siebert, Stefan

    2015-01-01

    We assessed the weather data aggregation effect (DAE) on the simulation of cropping systems for different crops, response variables, and production conditions. Using 13 processbased crop models and the ensemble mean, we simulated 30 yr continuous cropping systems for 2 crops (winter wheat and

  20. Characterization of degradation products of amorphous and polymorphic forms of clopidogrel bisulphate under solid state stress conditions

    DEFF Research Database (Denmark)

    Raijada, Dhara K; Prasad, Bhagwat; Paudel, Amrit

    2010-01-01

    The present study deals with the stress degradation studies on amorphous and polymorphic forms of clopidogrel bisulphate. The objective was to characterize the degradation products and postulate mechanism of decomposition of the drug under solid state stress conditions. For that, amorphous form, ...

  1. Technical snow production in skiing areas: conditions, practice, monitoring and modelling. A case study in Mayrhofen/Austria

    Science.gov (United States)

    Strasser, Ulrich; Hanzer, Florian; Marke, Thomas; Rothleitner, Michael

    2017-04-01

    The production of technical snow today is a self-evident feature of modern alpine skiing resort management. Millions of Euros are invested every year for the technical infrastructure and its operation to produce a homogeneous and continuing snow cover on the skiing slopes for the winter season in almost every larger destination in the Alps. In Austria, skiing tourism is a significant factor of the national economic structure. We present the framing conditions of technical snow production in the mid-size skiing resort of Mayrhofen (Zillertal Alps/Austria, 136 km slopes, elevation range 630 - 2.500 m a.s.l.). Production conditions are defined by the availability of water, the planned date for the season opening, and the climatic conditions in the weeks before. By means of an adapted snow production strategy an attempt is made to ecologically and economically optimize the use of water and energy resources. Monitoring of the snow cover is supported by a network of low-cost sensors and mobile snow depth recordings. Finally, technical snow production is simulated with the spatially distributed, physically based hydroclimatological model AMUNDSEN. The model explicitly considers individual snow guns and distributes the produced snow along the slopes. The amount of simulated snow produced by each device is a function of its type, of actual wet-bulb temperature at the location, of ski area infrastructure (in terms of water supply and pumping capacity), and of snow demand.

  2. Current status of the FASTGRASS/PARAGRASS models for fission product release from LWR fuel during normal and accident conditions

    International Nuclear Information System (INIS)

    Rest, J.; Zawadski, S.A.; Piasecka, M.

    1983-10-01

    The theoretical FASTGRASS model for the prediction of the behavior of the gaseous and volatile fission products in nuclear fuels under normal and transient conditions has undergone substantial improvements. The major improvements have been in the atomistic and bubble diffusive flow models, in the models for the behavior of gas bubbles on grain surfaces, and in the models for the behavior of the volatile fission products iodine and cesium. The thoery has received extensive verification over a wide range of fuel operating conditions, and can be regarded as a state-of-the-art model based on our current level of understanding of fission product behavior. PARAGRASS is an extremely efficient, mechanistic computer code with the capability of modeling steady-state and transient fission-product behavior. The models in PARAGRASS are based on the more detailed ones in FASTGRASS. PARAGRASS updates for the FRAPCON (PNL), FRAP-T (INEL), and SCDAP (INEL) codes have recently been completed and implemented. Results from an extensive FASTGRASS verification are presented and discussed for steady-state and transient conditions. In addition, FASTGRASS predictions for fission product release rate constants are compared with those in NUREG-0772. 21 references, 13 figures

  3. Development and Application of the Key Technologies for the Quality Control and Inspection of National Geographical Conditions Survey Products

    Science.gov (United States)

    Zhao, Y.; Zhang, L.; Ma, W.; Zhang, P.; Zhao, T.

    2018-04-01

    The First National Geographical Condition Survey is a predecessor task to dynamically master basic situations of the nature, ecology and human activities on the earth's surface and it is the brand-new mapping geographic information engineering. In order to ensure comprehensive, real and accurate survey results and achieve the quality management target which the qualified rate is 100 % and the yield is more than 80 %, it is necessary to carry out the quality control and result inspection for national geographical conditions survey on a national scale. To ensure that achievement quality meets quality target requirements, this paper develops the key technology method of "five-in-one" quality control that is constituted by "quality control system of national geographical condition survey, quality inspection technology system, quality evaluation system, quality inspection information management system and national linked quality control institutions" by aiming at large scale, wide coverage range, more undertaking units, more management levels, technical updating, more production process and obvious regional differences in the national geographical condition survey and combining with novel achievement manifestation, complicated dependency, more special reference data, and large data size. This project fully considering the domestic and foreign related research results and production practice experience, combined with the technology development and the needs of the production, it stipulates the inspection methods and technical requirements of each stage in the quality inspection of the geographical condition survey results, and extends the traditional inspection and acceptance technology, and solves the key technologies that are badly needed in the first national geographic survey.

  4. Screening of penicillium species and optimisation of culture conditions for the production of ergot alkaloids using surface culture fermentation process

    International Nuclear Information System (INIS)

    Shahid, M.G.

    2015-01-01

    Abstract. The present study deals with the screening of fungal species and suitable fermentation medium for the production of ergot alkaloids. Various species of genus Penicillium were grown on different fermentation media by employing surface culture fermentation technique to achieve the most suitable medium and the best Penicillium sp. The results showed that medium M5 gave maximum yield with Penicillium commune. Different culture conditions such as effect of different carbon and nitrogen sources, their concentration levels, different pH values and sizes of inoculum on the production of ergot alkaloids were also studied to improve the yield. Maximum production of ergot alkaloids (4.32 mg/L) was achieved with 15 mL spore suspension at pH 5 in fermentation medium containing 35% (w/v) sucrose. All these results indicate that culture conditions are very much crucial to improve the yield of ergot alkaloids produced by Penicillium commune through surface culture process. (author)

  5. Mechanistic prediction of fission product release under normal and accident conditions: key uncertainties that need better resolution

    International Nuclear Information System (INIS)

    Rest, J.

    1983-09-01

    A theoretical model has been used for predicting the behavior of fission gas and volatile fission products (VFPs) in UO 2 -base fuels during steady-state and transient conditions. This model represents an attempt to develop an efficient predictive capability for the full range of possible reactor operating conditions. Fission products released from the fuel are assumed to reach the fuel surface by successively diffusing (via atomic and gas-bubble mobility) from the grains to grain faces and then to the grain edges, where the fission products are released through a network of interconnected tunnels of fission-gas induced and fabricated porosity. The model provides for a multi-region calculation and uses only one size class to characterize a distribution of fission gas bubbles

  6. Optimization to the Culture Conditions for Phellinus Production with Regression Analysis and Gene-Set Based Genetic Algorithm

    Science.gov (United States)

    Li, Zhongwei; Xin, Yuezhen; Wang, Xun; Sun, Beibei; Xia, Shengyu; Li, Hui

    2016-01-01

    Phellinus is a kind of fungus and is known as one of the elemental components in drugs to avoid cancers. With the purpose of finding optimized culture conditions for Phellinus production in the laboratory, plenty of experiments focusing on single factor were operated and large scale of experimental data were generated. In this work, we use the data collected from experiments for regression analysis, and then a mathematical model of predicting Phellinus production is achieved. Subsequently, a gene-set based genetic algorithm is developed to optimize the values of parameters involved in culture conditions, including inoculum size, PH value, initial liquid volume, temperature, seed age, fermentation time, and rotation speed. These optimized values of the parameters have accordance with biological experimental results, which indicate that our method has a good predictability for culture conditions optimization. PMID:27610365

  7. Prediction of Hydrolysis Products of Organic Chemicals under Environmental pH Conditions

    Science.gov (United States)

    Cheminformatics-based software tools can predict the molecular structure of transformation products using a library of transformation reaction schemes. This paper presents the development of such a library for abiotic hydrolysis of organic chemicals under environmentally relevant...

  8. PRODUCTION OF THE STEEL CAST THERMO-TREATED GRIT IN CONDITIONS OF MINSK AUTOMOBILE PLANT

    Directory of Open Access Journals (Sweden)

    P. S. Gurchenko

    2004-01-01

    Full Text Available The technical and economic characteristic of the created and developed technological processes of the industrial production of the steel casting grit heat-treatable by induction heat is given.

  9. N2O production dynamics in nitrifying/denitrifying activated sludge under defined environmental conditions

    DEFF Research Database (Denmark)

    Pellicer i Nàcher, Carles; Jensen, Marlene Mark; Petersen, Morten S.

    contributor to global warming and the destruction of the ozone layer. The present study makes use of unique datasets collected during controlled batch tests with activated sludge biomass to test and calibrate a pseudo-mechanistic model that predicts N2O production by nitrifying and heterotrophic bacteria....... The proposed model described successfully the observed N2O production dynamics and confirmed that the availability of ammonia, low dissolved oxygen and nitrite accumulation were the main factors triggering N2O production. Nitrifier-denitrification was proposed as the main pathway catalyzing the conversion...... that a minor portion of the N2O produced was actually released to the gas phase. This work represents a step further in the use and calibration of process models to control and understand better N2O production and emissions during conventional wastewater treatment....

  10. Physical and enzymatic conditioning of oenological wastes to improve biogas production

    OpenAIRE

    Bracchitta, Mirko

    2012-01-01

    A fundamental assumption for by-product from winery industy waste-management is their economic and commercial increase in value. High energetic value recovery from winery industry is an attractive economic solution to stimulate new sustainable process. Approach of this work is based about physic and biological treatment with grape stalks and grape marc to increase polysaccharides components of cell wall and energetic availability of this by-products. Grape stalks for example have a high perce...

  11. Effect of Light Intensities and Atmospheric Gas Conditions on Biohydrogen Production of Microalgae Isolated from Fisheries Wastewater

    Directory of Open Access Journals (Sweden)

    Mujalin Pholchan

    2017-06-01

    Full Text Available Recently, the fishery farming industry has been developed rapidly due to increasing demand and consumption as well as the depletion of wild fish resources. Production processes in the industry usually generate large amounts of wastewater containing high nutrients, posing a threat to downstream water. However, phytoplankton removal techniques commonly used to counteract the threat, though appearing to have low efficiency, are timeconsuming and less sustainable. Microalgae are photosynthetic microorganisms that convert solar energy into hydrogen. Using the isolated algae from fish farms as a source of renewable energy production could be a promising choice for handling fisheries wastewater in a more efficient manner. However, hydrogen production processes from algae still need more studies as their efficiencies vary between algae species and growth factors. In this work, the efficiency of hydrogen production from Scenedesmus accuminatus and Arthrospira platensis harvested from fish farms under three different light intensity conditions and three atmospheric gas conditions was determined. The results showed that the best conditions for hydrogen production from both species included 24 h darkness and carbon dioxide addition. Under the atmospheric gas combination of 99% argon and 1% carbon dioxide, S. accuminatus could produce hydrogen gas as high as 0.572 mol H2/mgCh h within 12 h, while the highest hydrogen production (0.348 mol H2/mgCh h obtained from A. platensis was found under the atmospheric gas mixture of 98% argon and 2% carbon dioxide. Interestingly, S. accuminatus appeared to produce more hydrogen than A. platensis under the same conditions.

  12. Children Facial Expression Production: Influence of Age, Gender, Emotion Subtype, Elicitation Condition and Culture

    Directory of Open Access Journals (Sweden)

    Charline Grossard

    2018-04-01

    Full Text Available The production of facial expressions (FEs is an important skill that allows children to share and adapt emotions with their relatives and peers during social interactions. These skills are impaired in children with Autism Spectrum Disorder. However, the way in which typical children develop and master their production of FEs has still not been clearly assessed. This study aimed to explore factors that could influence the production of FEs in childhood such as age, gender, emotion subtype (sadness, anger, joy, and neutral, elicitation task (on request, imitation, area of recruitment (French Riviera and Parisian and emotion multimodality. A total of one hundred fifty-seven children aged 6–11 years were enrolled in Nice and Paris, France. We asked them to produce FEs in two different tasks: imitation with an avatar model and production on request without a model. Results from a multivariate analysis revealed that: (1 children performed better with age. (2 Positive emotions were easier to produce than negative emotions. (3 Children produced better FE on request (as opposed to imitation; and (4 Riviera children performed better than Parisian children suggesting regional influences on emotion production. We conclude that facial emotion production is a complex developmental process influenced by several factors that needs to be acknowledged in future research.

  13. Physical robustness of canopy temperature models for crop heat stress simulation across environments and production conditions

    DEFF Research Database (Denmark)

    Webber, Heidi; White, Jeffrey W; Kimball, Bruce

    2018-01-01

    to simulate Tc. Model performance in predicting Tc was evaluated for two experiments in continental North America with various water, nitrogen and CO2 treatments. An empirical model fit to one dataset had the best performance, followed by the EBSC models. Stability conditions explained much of the differences...... between modeling approaches. More accurate simulation of heat stress will likely require use of energy balance approaches that consider atmospheric stability conditions....

  14. Fate of Pharmaceuticals and Personal Care Products (PPCPs) in Saturated Soil Under Various Redox Conditions

    Science.gov (United States)

    Dror, I.; Menahem, A.; Berkowitz, B.

    2014-12-01

    The growing use of PPCPs results in their increasing release to the aquatic environment. Consequently, understanding the fate of PPCPs under environmentally relevant conditions that account for dynamic flow and varying redox states is critical. In this study, the transport of two organometallic PPCPs, Gd-DTPA and Roxarsone (As complex) and their metal salts (Gd(NO3)3, AsNaO2), is investigated. The former is used widely as a contrasting agent for MRI, while the latter is applied extensively as a food additive in the broiler poultry industry. Both of these compounds are excreted from the body, almost unchanged chemically. Gadolinium complexes are not fully eliminated in wastewater treatment and can reach groundwater via irrigation with treated wastewater; Roxarsone can enter groundwater via leaching from manure used as fertilizer. Studies have shown that the transport of PPCPs in groundwater is affected by environmental conditions such as redox states, pH, and soil type. For this study, column experiments using sand or Mediterranean red sandy clay soil were performed under several redox conditions: aerobic, nitrate-reducing, iron-reducing, sulfate-reducing, methanogenic, and very strongly chemical reducing. Batch experiments to determine adsorption isotherms were also performed for the complexes and metal salts. We found that Gd-DTPA transport was affected by the soil type and was not affected by the redox conditions. In contrast, Roxarsone transport was affected mainly by the different redox conditions, showing delayed breakthrough curves as the conditions became more biologically reduced (strong chemical reducing conditions did not affect the transport). We also observed that the metal salts show essentially no transport while the organic complexes display much faster breakthrough. The results suggest that transport of these PPCPs through soil and groundwater is determined by the redox conditions, as well as by soil type and the form of the applied metal (as salt

  15. Effect of operating conditions in production of diagnostic Salmonella Enteritidis O-antigen-specific monoclonal antibody in different bioreactor systems.

    Science.gov (United States)

    Ayyildiz-Tamis, Duygu; Nalbantsoy, Ayse; Elibol, Murat; Deliloglu-Gurhan, Saime Ismet

    2014-01-01

    In this study, different cultivation systems such as roller bottles (RB), 5-L stirred-tank bioreactor (STR), and disposable bioreactors were used to cultivate hybridoma for lab-scale production of Salmonella Enteritidis O-antigen-specific monoclonal antibody (MAb). Hybridoma cell line was cultivated in either serum-containing or serum-free medium (SFM) culture conditions. In STR, MAb production scaled up to 4 L, and production capabilities of the cells were also evaluated in different featured production systems. Moreover, the growth parameters of the cells in all production systems such as glucose consumption, lactate and ammonia production, and also MAb productivities were determined. Collected supernatants from the reactors were concentrated by a cross-flow filtration system. In conclusion, cells were not adapted to SFM in RB and STR. Therefore, less MAb titer in both STR and RB systems with SFM was observed compared to the cultures containing fetal bovine serum-supplemented medium. A higher MAb titer was gained in the membrane-aerated system compared to those in STR and RB. Although the highest MAb titer was obtained in the static membrane bioreactor system, the highest productivity was obtained in STR operated in semicontinuous mode with overlay aeration.

  16. Grain boundary sweeping and dissolution effects on fission product behaviour under severe fuel damage accident conditions

    International Nuclear Information System (INIS)

    Rest, J.

    1986-01-01

    The theoretical FASTGRASS-VFP model has been used in the interpretation of fission gas, iodine, tellurium, and cesium release from severe-fuel-damage (SFD) tests performed in the PBF reactor in Idaho. A theory of grain boundary sweeping of gas bubbles, gas bubble behavior during fuel liquefaction (destruction of grain boundaries due to formation of a U-rich melt phase), and during U-Zr eutectic melting has been included within the FASTGRASS-VFP formalism. The grain-boundary-sweeping theory considers the interaction between the moving grain boundary and two distinct size classes of bubbles, those on grain faces and on grain edges. The theory of the effects of fuel liquefaction and U-Zr eutectic melting on fission product behaviour considers the migration and coalescence of fission gas bubbles in either molten uranium, or a Zircaloy-Uranium eutectic melt. Results of the analyses demonstrate that intragranular fission product behavior during the tests can be interpreted in terms of a grain-growth/grain-boundary-sweeping mechanism that enhances the flow of fission products from within the grains to the grain boundaries. Whereas fuel liquefaction leads to an enhanced release of fission products in trace-irradiated fuel, the occurrence of fuel liquefaction in normally-irradiated fuel can degrade fission product release. This phenomenon is due in part to reduced gas-bubble mobilities in a viscous medium as compared to vapor transport, and in part to a degradation of grain growth rates and the subsequent decrease in grain-boundary sweeping of intragranular fission products into the liquified lamina. The analysis shows that total UO 2 dissolution due to eutectic melting leads to increased release for both trace-irradiated and normally-irradiated fuel. The FASTGRASS-VFP predictions, measured release rates from the above tests, and previously published release rates are compared and differences between fission product behavior in trace-irradiated and in normally

  17. Improvement of d-Lactic Acid Production in Saccharomyces cerevisiae Under Acidic Conditions by Evolutionary and Rational Metabolic Engineering.

    Science.gov (United States)

    Baek, Seung-Ho; Kwon, Eunice Y; Bae, Sang-Jeong; Cho, Bo-Ram; Kim, Seon-Young; Hahn, Ji-Sook

    2017-10-01

    Microbial lactic acid (LA) production under acidic fermentation conditions is favorable to reduce the production cost, but circumventing LA toxicity is a major challenge. A d-LA-producing Saccharomyces cerevisiae strain JHY5610 is generated by expressing d-lactate dehydrogenase gene (Lm. ldhA) from Leuconostoc mesenteroides, while deleting genes involved in ethanol production (ADH1, ADH2, ADH3, ADH4, and ADH5), glycerol production (GPD1 and GPD2), and degradation of d-LA (DLD1). Adaptive laboratory evolution of JHY5610 lead to a strain JHY5710 having higher LA tolerance and d-LA-production capability. Genome sequencing of JHY5710 reveal that SUR1 I245S mutation increases LA tolerance and d-LA-production, whereas a loss-of-function mutation of ERF2 only contributes to increasing d-LA production. Introduction of both SUR1 I245S and erf2Δ mutations into JHY5610 largely mimic the d-LA-production capability of JHY5710, suggesting that these two mutations, which could modulate sphingolipid production and protein palmitoylation, are mainly responsible for the improved d-LA production in JHY5710. JHY5710 is further improved by deleting PDC1 encoding pyruvate decarboxylase and additional integration of Lm. ldhA gene. The resulting strain JHY5730 produce up to 82.6 g L -1 of d-LA with a yield of 0.83 g g -1 glucose and a productivity of 1.50 g/(L · h) in fed-batch fermentation at pH 3.5. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  18. Fertility and productivity index rating of some soils conditioned by climate and topography

    International Nuclear Information System (INIS)

    Okae-Anti, Daniel; Imoro, Abukari Z.

    2004-10-01

    In the humid tropics, well-drained soils with abundant loose iron concretions, soils with very little weatherable minerals left and of poor chemical status due to the abundance of low activity clays, pose serious limitations to agricultural productivity. Accurate and reliable soil productivity information is needed in the form of crop yield estimates and productivity indices. We investigated the productive potential of some acrisols and plinthosols by computing productivity indices (PI) and estimating yields of four test crops, namely cowpea, groundnut, maize and soybean. PI was based on physico-chemical parameters expected to regulate air-water relations, have a role in determining the mechanical resistance to crop roots and the volume of soil, and, contribute to the fertility status of the soils. Very low Pls in the order 7.2x10 - 5, 9.6x10 - 5 and 8.32x10 - 4 were observed for Nyankpala, Changnayili and Kpelesawgu series respectively. Based on the inherently low fertility status of these soils, maize, planted as a sole crop or in rotation with groundnut is the sustainable cropping system for the soils. A return of crop residue to the soil is envisaged as a long term practice to maintain adequate levels of organic matter. (author)

  19. CONDITIONING MICROBIAL PRODUCTS CONTAINING LACTIC BACTERIA WITH ORGANIC AND INORGANIC SUPPORTS FOR USE IN ANIMAL FEEDING

    Directory of Open Access Journals (Sweden)

    T VINTILĂ

    2007-05-01

    Full Text Available The stability in real time of three strains of lactic bacteria (Lactobacillus acidophilus, Lactobacillus plantarum, Enterococcus faecium mixed with different excipients was evaluated during a 6-months period. The excipients studied were: zeolite, calcium carbonate, perlite ceramic, wheat bran and Carboxymethyl cellulose (CMC. A part of liquid cultures and excipients mixtures were dried (12- 14% humidity and other part were sealed in plastic bags with over 70% humidity and preserved at +4oC. The cells were activated periodically by suspending aliquots from wet and dry products in 0.9% saline solution. The viability of lactic bacteria was evaluated by cultivation of diluted suspensions in MRS plates. The number of viable lactic cells is decreasing very slowly, or remains constant in calcium carbonate, ceramics and CMC dry products for al strains. In the case of zeolite, the viability of Lb. acidophilus and Lb. plantarum decrease to 0 in the first month, and the viability of Ec. faecium decrease 20 times in 6 months. As for wet products, the number of viable cells is increasing in the first 30 days for calcium carbonate and in the first 60 days for wheat bran. The numbers of viable cells decrease in both wet products, reaching values close to the viability in fresh products after 6 months of storage at +4oC.

  20. Enhancement of docosahexaenoic acid (DHA) production from Schizochytrium sp. S31 using different growth medium conditions.

    Science.gov (United States)

    Sahin, Deniz; Tas, Ezgi; Altindag, Ulkü Hüma

    2018-01-24

    Schizochytrium species is one of the most studied microalgae for production of docosahexaenoic acid (DHA) which is an omega-3 fatty acid with positive effects for human health. However, high cost and low yield in production phase makes optimization of cultivation process inevitable. We focus on the optimization of DHA production using Schizochytrium sp. using different media supplements; glucose, fructose and glycerol as carbon variants, proteose peptone and tryptone as nitrogen variants. The highest biomass (5.61 g/L) and total fatty acid yield (1.74 g/L) were obtained in proteose peptone medium which was used as the alternative nitrogen source instead of yeast extract. The highest DHA yield (0.40 g/L) was achieved with glycerol as the carbon source although it had the second lowest biomass production after ethanol containing medium. Ethanol, as an alternative carbon source and a precursor for acetyl-CoA, increased DHA percentage in total lipid content from 29.94 to 40.04% but decreasing the biomass drastically. Considering different carbon and nitrogen sources during cultivation of Schizochytrium sp. will improve DHA production. Combination of proteose peptone and glycerol as nitrogen and carbon sources, respectively, and addition of ethanol with a proper timing will be useful to have higher DHA yield.

  1. Production of polyhydroxybutyrate and alginate from glycerol by Azotobacter vinelandii under nitrogen-free conditions.

    Science.gov (United States)

    Yoneyama, Fuminori; Yamamoto, Mayumi; Hashimoto, Wataru; Murata, Kousaku

    2015-01-01

    Glycerol is an interesting feedstock for biomaterials such as biofuels and bioplastics because of its abundance as a by-product during biodiesel production. Here we demonstrate glycerol metabolism in the nitrogen-fixing species Azotobacter vinelandii through metabolomics and nitrogen-free bacterial production of biopolymers, such as poly-d-3-hydroxybutyrate (PHB) and alginate, from glycerol. Glycerol-3-phosphate was accumulated in A. vinelandii cells grown on glycerol to the exponential phase, and its level drastically decreased in the cells grown to the stationary growth phase. A. vinelandii also overexpressed the glycerol-3-phosphate dehydrogenase gene when it was grown on glycerol. These results indicate that glycerol was first converted to glycerol-3-phosphate by glycerol kinase. Other molecules with industrial interests, such as lactic acid and amino acids including γ-aminobutyric acid, have also been accumulated in the bacterial cells grown on glycerol. Transmission electron microscopy revealed that glycerol-grown A. vinelandii stored PHB within the cells. The PHB production level reached 33% per dry cell weight in nitrogen-free glycerol medium. When grown on glycerol, alginate-overproducing mutants generated through chemical mutagenesis produced 2-fold the amount of alginate from glycerol than the parental wild-type strain. To the best of our knowledge, this is the first report on bacterial production of biopolymers from glycerol without addition of any nitrogen source.

  2. Reliability of offshore wind power production under extreme wind conditions. Deliverable D 9.5. Work Package 9: Electrical grid

    DEFF Research Database (Denmark)

    Cutululis, Nicolaos Antonio; Zeni, Lorenzo

    years, with each year simulated with five random seeds, leading to a total of 25 annual wind power time series for six large offshore wind farms, summing up to a little over 330 wind turbines. Two storm control strategies were used. The analysis involved several aspects inspired from reliability studies....... The aspects investigated are storm events occurrences and durations, storm control strategy impact on the capacity factor (lost production), the loss of production (power produced from wind drops below a certain threshold due to high wind speeds and storm controller) and finally, the wind power production......Reliability of offshore wind production under extreme wind conditions was investigated in this report. The wind power variability from existing and future large offshore wind farms in Western Denmark were simulated using the Correlated Wind model developed at Risø. The analysis was done for five...

  3. Comparison of hydrogen-production capability of four different Enterobacteriaceae strains under growing and non-growing conditions

    Energy Technology Data Exchange (ETDEWEB)

    Seol, Eunhee; Kim, Seohyoung; Raj, S. Mohan; Park, Sunghoon [Department of Chemical and Biochemical Engineering, Pusan National University, Busan 609-735 (Korea)

    2008-10-15

    Non-growing cells can function as whole-cell biocatalysts for hydrogen (H{sub 2}) production, a process that has recently drawn much attention. In order to evaluate their potential as whole-cell biocatalysts, we compared the H{sub 2}-production capability of four Enterobacteriaceae strains (Citrobacter amalonaticus Y19, Escherichia coli K-12 MG1655, Escherichia coli DJT135, and Enterobacter aerogenes) under growing and non-growing conditions. We evaluated their H{sub 2}-production activity at varying temperatures (25-45 C) and pH conditions (6.0-8.0) using glucose or formate as the carbon source. Under growing conditions with 10 mM glucose as a substrate, E. aerogenes exhibited the highest H{sub 2}-production activity (17.0 {+-} 0.2 {mu}mol H{sub 2} mg cell{sup -1} h{sup -1}) among the four strains, but the final H{sub 2} yield was similar (1.7-1.8 mol H{sub 2} mol{sup -1} glucose) in all four strains. H{sub 2} production in the four strains proceeded through a formate-dependent pathway that involved the formate hydrogen lyase (FHL) complex. Under non-growing conditions with 20 mM formate as a substrate, we obtained high H{sub 2}-production activities, in the range of 95.5-195.2 {mu}mol H{sub 2} mg cell{sup -1} h{sup -1}, with E. coli DJT135 exhibiting the highest activity (195.2 {mu}mol H{sub 2} mg{sup -1} h{sup -1}) at pH 6.0 and 45 C. In contrast, using glucose as the carbon substrate in non-growing cell experiments greatly reduced the H{sub 2}-production activity to 6.1-7.7 {mu}mol H{sub 2} mg cell{sup -1} h{sup -1}. This study indicated that formate is a better substrate than glucose for H{sub 2} production by non-growing cells, and that the H{sub 2}-production performance among the strains did not vary significantly, with the exception of E. coli K-12 MG1655. (author)

  4. Harbor porpoise clicks do not have conditionally minimum time bandwidth product

    DEFF Research Database (Denmark)

    Beedholm, Kristian

    2008-01-01

    The hypothesis that odontocete clicks have minimal time frequency product given their delay and center frequency values is tested by using an in-phase averaged porpoise click compared with a pure tone weighted with the same envelope. These signals have the same delay and the same center frequency...... values but the time bandwidth product of the artificial click is only 0.76 that of the original. Therefore signals with the same parameters exist that have a lower time bandwidth product. The observation that porpoise clicks are in fact minimum phase is confirmed for porpoise clicks and this property...... is argued to be incompatible with optimal reception, if auditory filters are also minimum phase....

  5. Effect of operating conditions in soil aquifer treatment on the removals of pharmaceuticals and personal care products

    Energy Technology Data Exchange (ETDEWEB)

    He, Kai, E-mail: hekai@urban.env.kyoto-u.ac.jp; Echigo, Shinya; Itoh, Sadahiko

    2016-09-15

    Soil aquifer treatment (SAT) is an alternative advanced treatment for wastewater reclamation, and it has the potential to control micropollutants including pharmaceuticals and personal care products (PPCPs). However, the relationship of operating conditions in SAT and removals of micropollutants was not clear. In this study, the effects of operating conditions on the removals of PPCPs were evaluated by using lab-scale columns and plant pilot-scale reactors under different operating conditions. Firstly, weathered granite soil (WGS), standard sand (SAND) and Toyoura standard sand (TS) have different soil characteristics such as total organic carbon (TOC) and cation exchange capacity (CEC). In the columns with these packing materials, the removals of carboxylic analgesics and antilipidemics were effective regardless packing materials. The removals of antibiotics were more effective in WGS than in TS and SAND, indicating high TOC and CEC enhance the sorption in SAT. Secondly, with the extension of hydraulic retention time (HRT), the removals of sulfamethoxazole, acetaminophen, crotamiton, and antipyrine were improved in WGS columns, and adaptable biodegradation for moderately removable PPCPs was formed. Thirdly, the removal efficiencies of sulfamethoxazole and crotamiton were higher in the WGS column under vadose condition than in the WGS column under saturated condition, because of aerobic condition in WGS column under vadose condition. Though long HRT and vadose condition had positive influence on the removals of several PPCPs such as sulfamethoxazole, WGS column with an HRT of 7 days under saturated condition removed most PPCPs. - Highlights: • Soil organic matter and cation exchange capacity enhanced the removals of antibiotics in SAT. • A hydraulic retention time (HRT) of 7 days was sufficient for the removals of most PPCPs. • The removals of most selected PPCPs were similar under vadose and saturated conditions. • Vadose condition contributed to the

  6. Small Ruminant Production System Efficiency under Abu-Dhabi, United Arab Emirates Arid Land Conditions

    Directory of Open Access Journals (Sweden)

    Eihab Fathelrahman

    2014-12-01

    Full Text Available Sheep and goat production systems in the United Arab Emirates (UAE operate under scarce natural resource constraints. A cross-sectional survey that covered 661 mixed farms, including major sheep and goat production, was conducted in the three regions of Abu Dhabi Emirate (Al-Ain, Western Region and Abu Dhabi city during 2012. A Cobb-Douglas, double-logarithmic stochastic frontier production function and maximum likelihood estimation were applied to estimate important economic derivatives and the associated risk of small ruminant production in this arid area. The highest impact of an input on the output level was found to be labor for raising sheep and alfalfa grass for raising goats. Both labor and alfalfa variables were found to be overutilized for sheep and goat production, respectively. Overall, the results indicate that average technical efficiency is 0.62 for raising sheep and only 0.34 for raising goats in the study area. Technical efficiency analysis included measuring the frequency of farms at each level of estimated technical efficiency in the range between zero and one. Zero for the technical efficiency coefficient indicates a lack of technical efficiency in resource use. The results of this study indicated that only 1% of the sheep farms show a technical efficiency coefficient of 0.25 or less; the same can be said for 41% of goat producers. However, these technical efficiencies were found to be more than 0.75 for 12% and 5% of the sheep and goat farms, respectively. Overall, goat farming in the UAE was found to be less efficient than sheep production. The results also indicated that flock size and type of breed were the most influential factors relative to other factors, and both show a positive relationship with technical efficiency. Other than flock size, factors, such as owners’ years of experience and management practices, were found to be more influential on goat farming system efficiency relative to sheep farming.

  7. Hydrogen production under salt stress conditions by a freshwater Rhodopseudomonas palustris strain.

    Science.gov (United States)

    Adessi, Alessandra; Concato, Margherita; Sanchini, Andrea; Rossi, Federico; De Philippis, Roberto

    2016-03-01

    Hydrogen represents a possible alternative energy carrier to face the growing request for energy and the shortage of fossil fuels. Photofermentation for the production of H2 constitutes a promising way for integrating the production of energy with waste treatments. Many wastes are characterized by high salinity, and polluted seawater can as well be considered as a substrate. Moreover, the application of seawater for bacterial culturing is considered cost-effective. The aims of this study were to assess the capability of the metabolically versatile freshwater Rhodopseudomonas palustris 42OL of producing hydrogen on salt-containing substrates and to investigate its salt stress response strategy, never described before. R. palustris 42OL was able to produce hydrogen in media containing up to 3 % added salt concentration and to grow in media containing up to 4.5 % salinity without the addition of exogenous osmoprotectants. While the hydrogen production performances in absence of sea salts were higher than in their presence, there was no significant difference in performances between 1 and 2 % of added sea salts. Nitrogenase expression levels indicated that the enzyme was not directly inhibited during salt stress, but a regulation of its expression may have occurred in response to salt concentration increase. During cell growth and hydrogen production in the presence of salts, trehalose was accumulated as a compatible solute; it protected the enzymatic functionality against salt stress, thus allowing hydrogen production. The possibility of producing hydrogen on salt-containing substrates widens the range of wastes that can be efficiently used in production processes.

  8. On the assessment of the productivity of suspension cultures of unicellular green algae at defined light conditions

    Energy Technology Data Exchange (ETDEWEB)

    Seiss, U; Borns, E; Boehm, H

    1985-01-01

    A description is given of the possibility of a comparison of the production between different suspension cultures of microalgae at equal and defined light conditions. For this, a variant of the turbidostat technique is used by which with the acid of a phototransistor and the filter combination of red filter/opal glass filter a chlorophyll-equivalent signal is applied for control. The equal light conditions are compared and set by the preparation of the respective absorption profiles. From this one can derive the mean level of irradiation within the suspension cultures and use it as the reference value for the light conditions. By this technique it is possible to set equal light conditions in suspension cultures independent of the given cell sizes, cell shapes and dry matter contents.

  9. High Temperature Corrosion under Laboratory Conditions Simulating Biomass-Firing: A Comprehensive Characterization of Corrosion Products

    DEFF Research Database (Denmark)

    Okoro, Sunday Chukwudi; Montgomery, Melanie; Jappe Frandsen, Flemming

    2014-01-01

    characterization of the corrosion products. The corrosion products consisted of three layers: i) the outermost layer consisting of a mixed layer of K2SO4 and FexOy on a partly molten layer of the initial deposit, ii) the middle layer consists of spinel (FeCr2O4) and Fe2O3, and iii) the innermost layer is a sponge......-like Ni3S2 containing layer. At the corrosion front, Cl-rich protrusions were observed. Results indicate that selective corrosion of Fe and Cr by Cl, active oxidation and sulphidation attack of Ni are possible corrosion mechanisms....

  10. Product development tailored to fit the economic conditions in the healthcare system

    International Nuclear Information System (INIS)

    Kittner, T.; Niederlag, W.; Schiffer, J.G.V.; Standke, R.

    2007-01-01

    Product development in medical technology is taking place likewise for hardware and software. A cost-effective way to improve the performance of existing medical products is the development of dedicated easy upgradable software. Installing additional complex reconstruction algorithm packages can improve the sensitivity and spatial resolution of PET scanners or SPECT systems dramatically. The rapid improving computer power today allows the implementation of computational very intensive algorithms. For three applications the advantage of pure software implementations in the daily routine is shown. (orig.)

  11. Response of Xylella fastidiosa to zinc: decreased culturability, increased exopolysaccharide production, and formation of resilient biofilms under flow conditions.

    Science.gov (United States)

    Navarrete, Fernando; De La Fuente, Leonardo

    2014-02-01

    The bacterial plant pathogen Xylella fastidiosa produces biofilm that accumulates in the host xylem vessels, affecting disease development in various crops and bacterial acquisition by insect vectors. Biofilms are sensitive to the chemical composition of the environment, and mineral elements being transported in the xylem are of special interest for this pathosystem. Here, X. fastidiosa liquid cultures were supplemented with zinc and compared with nonamended cultures to determine the effects of Zn on growth, biofilm, and exopolysaccharide (EPS) production under batch and flow culture conditions. The results show that Zn reduces growth and biofilm production under both conditions. However, in microfluidic chambers under liquid flow and with constant bacterial supplementation (closer to conditions inside the host), a dramatic increase in biofilm aggregates was seen in the Zn-amended medium. Biofilms formed under these conditions were strongly attached to surfaces and were not removed by medium flow. This phenomenon was correlated with increased EPS production in stationary-phase cells grown under high Zn concentrations. Zn did not cause greater adhesion to surfaces by individual cells. Additionally, viability analyses suggest that X. fastidiosa may be able to enter the viable but nonculturable state in vitro, and Zn can hasten the onset of this state. Together, these findings suggest that Zn can act as a stress factor with pleiotropic effects on X. fastidiosa and indicate that, although Zn could be used as a bactericide treatment, it could trigger the undesired effect of stronger biofilm formation upon reinoculation events.

  12. Milk Production, Physiological Condition and Performance of Etawa Crossbreed Goats Feed by Ration Supplemented with Mangosteen Peel Flour

    Science.gov (United States)

    Dzarnisa; Rachmadi, D.; Azhar, A.; Fakhrur Riza, R.; Hidayati, A.

    2018-02-01

    Study on the effect of the addition of mangosteen (Garcinia mangostana L.) peel flour on physiological condition and performance of Etawa crossbreed goats was done. This was to grant the use of mangosteen peel flour that rich of antioxidants and has variety good benefits for health as feed additive for cattle. This study used a Complete Randomized Block Design consisting of 4 treatment groups and 4 replications each. Subjects were 16 female Etawa crossbreed goats randomly designed into treatments group based on lactation periods. Subjects were feed with traditional rations (control, A), traditional rations and 2.5% mangosteen peel flour (B), tradition rations and 5% mangosteen peel flour (C), and traditional rations and 7,5 % mangosteen peel flour (D). Data on performance (milk production) and physiological condition (respiratory frequency, rectal temperature, and heart rate) obtained were analyzed using analysis of variance (ANOVA). The results showed that the addition of mangosteen peel flour as food additive in the rations resulted in variations in the milk production, physiological condition (rectal temperature, heart rate and respiration frequency) and performances (daily weigh gain, food consumption, ration conversion and breast volume) of Etawa crossbreed goats, but significant effect was only observed in the respiration frequency. The addition of 2.5% mangosteen peel flour in the ration caused the best, expected effects on milk production physiological condition and performance of Etawa crossbreed goats.

  13. Adaptive estimation of multivariate functions using conditionally Gaussian tensor-product spline priors

    NARCIS (Netherlands)

    Jonge, de R.; Zanten, van J.H.

    2012-01-01

    We investigate posterior contraction rates for priors on multivariate functions that are constructed using tensor-product B-spline expansions. We prove that using a hierarchical prior with an appropriate prior distribution on the partition size and Gaussian prior weights on the B-spline

  14. Organisation of working time: Implications for productivity and working conditions. Overview Report

    NARCIS (Netherlands)

    Goudswaard, A.; Dhondt, S.; Vergeer, R.; Oeij, P.; Leede, J. de; Adrichem, K. van C; Csizmadia, P.; Makó, C.; Illésy, M.; Tóth, A.

    2012-01-01

    Companies in search of improved productivity use a wide range of approaches, from financial incentives and skills upgrading and training to increased autonomy of individuals and teams. Working time flexibility has the added advantage in that it can benefit both workers and employers: it gives

  15. Optimizing culture conditions for the production of endo-β-1,4 ...

    African Journals Online (AJOL)

    Among tested carbon sources (coconut fiber, coffee shell, corncob, dried tangerine skin, peanut shell, rice bran, saw dust, sugar-cane bagasse as organic wasters and glucose, lactose sucrose as pure carbon sources), corncob showed the highest endoglucanase production by A. awamori VTCC-F099 at the concentration ...

  16. In vitro wheat haploid embryo production by wheat x maize cross system under different environmental conditions

    International Nuclear Information System (INIS)

    Khan, M.A.; Ahmad, J.

    2011-01-01

    Haploids are helpful in studies for inter genomic relationship, identifying molecular markers, reducing time period of varietal development and increasing efficiency of breeding program. In case of bread wheat (Triticum aestivum L.), wheat x maize cross system is the most successful system due to its higher efficiency, more haploid embryo production and low genetic specificity. The haploid embryo production is affected by many factors i.e. light, temperature, relative humidity and tiller culture media. A study was carried out comprising 25 genotypes of bread wheat for haploid embryo production using 100 mgL/sup -1/ 2,4-D, 40Gl/sup -1/ Sucrose and 8mlL/sup -1/ Sulphurous acid. Haploid embryo production was observed at various levels of environmental factors i.e. maize pollen collection temperature, time of pollination after tiller emasculation, light intensity and relative humidity during haploid seed formation. Maximum haploid embryo formation recorded was 9.52%. Best temperature observed for pollination was 21-26 degree C, optimum time duration for pollination was 24 hours after emasculation, light intensity was 10,000 Lux and relative humidity was 60-65% at 20-22 degree C. (author)

  17. Optimization of the conditions for the precipitation of thorium oxalate. II. Minimization of the product losses

    International Nuclear Information System (INIS)

    Pazukhin, E.M.; Smirnova, E.A.; Krivokhatskii, A.S.; Pazukhina, Yu.L.; Kiselev, P.P.

    1987-01-01

    The precipitation of thorium as a poorly soluble oxalate was investigated. An equation relating the concentrations of the metal and nitric acid in the initial solution and the amount of precipitant required to minimize the product losses was derived. A graphical solution of the equation is presented for the case where the precipitant is oxalic acid at a concentration of 0.78 M

  18. Steel corrosion products solubility under conditions simulating various water chemistry parameters in power plants

    International Nuclear Information System (INIS)

    Slobodov, A.A.; Kritskij, V.G.; Zarembo, V.I.; Puchkov, L.V.

    1988-01-01

    To simulate construction material corrosion product mass transfer model in power plant circuits calculation of iron oxide and hydroxide solubility, depending on water chemistry parameters: temperature, pH-value, content of dissolved in water hydrogen and oxygen, is carried out

  19. 27 CFR 11.23 - Sales conditioned on the acquisition of other products.

    Science.gov (United States)

    2010-04-01

    ... TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS CONSIGNMENT SALES Unlawful Sales Arrangements... of the sale involves, directly or indirectly, the acquisition by the industry member from the trade... or implied privilege of return extended when the product was originally sold. Industry members may...

  20. Wood pellet production costs under Austrian and in comparison to Swedish framework conditions

    NARCIS (Netherlands)

    Obernberger, I.; Thek, G.

    2004-01-01

    Owing to the rapidly increasing importance of pellets as high-quality biomass fuel in Austria and Europe within the last years, many companies, mainly from the wood industry, are thinking of entering this market. The calculation of the production costs before starting a pellet plant is essential for

  1. Comparative economic assessment of ethanol production under mesophilic and thermophilic conditions

    International Nuclear Information System (INIS)

    Mistry, P.B.

    1991-01-01

    Key technical factors affecting the economics of bioethanol production are critically analyzed with special reference to the relative merits of thermophilic and mesophilic fermentation. A number of novel process schemes to take advantage of thermophilic operation are discussed. Analysis of the capital and operating costs for a range of flowsheets then provides a basis for critical study. Estimates for thermophilic production are compared with those for a sugar cane based mesophilic process (using S. cerevisiae). For the thermophilic fermentation, the basic kinetic and yield constants are based on projected values for a strain of B. stearothermophilus. Compared to mesophilic operation, thermophilic operation results in reduced capital, operating and feed costs. The feed cost still accounts for a large proportion (75%) of the total production cost. However, on a feed-cost-free basis, a reduction in production cost of up to 32% could be realized by changing to thermophilic operation from existing yeast-based processes, after minor process modifications. 20 refs., 10 figs., 8 tabs

  2. Microstructural evolution adjacent to grain boundaries under cascade damage conditions and helium production

    DEFF Research Database (Denmark)

    Trinkaus, H.; Singh, B.N.; Victoria, M.

    1996-01-01

    the cascade damage is accompanied by a high helium production rate. It is shown that, in this case, the width of the peak zone is controlled by the (mostly invisible) bubble structure rather than by the (visible) void structure. The reduced swelling relative to that under neutron irradiation is attributed...

  3. Exploring optimal conditions for thermophilic fermentative hydrogen production from cassava stillage

    Energy Technology Data Exchange (ETDEWEB)

    Luo, Gang; Zou, Zhonghai; Wang, Wen [Key Laboratory of Yangtze River Water Environment, Ministry(Tongji University), Siping Road no 1239, Shanghai 200092 (China); Xie, Li; Zhou, Qi [Key Laboratory of Yangtze River Water Environment, Ministry(Tongji University), Siping Road no 1239, Shanghai 200092 (China); UNEP-Tongji University Institute of Environment for Sustainable Development, Siping Road no 1239, Shanghai 200092 (China)

    2010-06-15

    This study investigated the effects of seed sludges, alkalinity and HRT on the thermophilic fermentative hydrogen production from cassava stillage. Five different kinds of sludges were used as inocula without any pretreatment. Though batch experiments showed that mesophilic anaerobic sludge was the best inoculum, the hydrogen yields with different seed sludges were quite similar in continuous experiments in the range of 82.9-92.3 ml H{sub 2}/gVS without significant differences which could be attributed to the establishment of Uncultured Thermoanaerobacteriaceae bacterium-dominant microbial communities in all reactors. It is indicated that results obtained from batch experiments are not consistent with those from continuous experiments and all the tested seed sludges are good sources for continuous thermophilic hydrogen production from cassava stillage. The influent alkalinity of 6 g NaHCO{sub 3}/L and HRT 24 h were optimal for hydrogen production with hydrogen yield of 76 ml H{sub 2}/gVS and hydrogen production rate of 3215 ml H{sub 2}/L/d. Butyrate was the predominant metabolite in all experiments. With the increase in alkalinity of more than 6 g/L, the concentration of VFA/ethanol increased while hydrogen yield decreased due to the higher concentration of acetate and propionate. The decrease in HRT resulted in the higher hydrogen production rate but lower hydrogen yield. Variation of hydrogen yields were quite correlated with butyrate/acetate (B/A) ratio with different influent alkalinities, however, butyrate was important parameter to justify the hydrogen yields with various HRTs. (author)

  4. Production bias and void swelling in the transient regime under cascade damage conditions

    International Nuclear Information System (INIS)

    Singh, B.N.; Foreman, A.J.E.

    1992-01-01

    Molecular dynamics (MD) studies of collision cascades have firmly established that, in addition to clusters of vacancies, clusters of self-interstitial atoms (SIAs) are formed within the cascade volume during the thermal spike phase of a cascade. These clusters are formed in a segregated fashion such that the vacancy-rich core is surrounded by SIA clusters. At temperatures above stage V the vacancies evaporate from the vacancy cluster and diffuse into the medium whereas the SIA clusters remain thermally stable at temperatures even above the peak swelling temperature. This asymmetry in the production of free and mobile vacancies and SIAs gives rise to a production bias. Some of the vacancies evaporating from the vacancy-rich core annihilate at the SIA clusters surrounding it and others escape into the medium. It is this escaping fraction of vacancies which determines the strength of the production bias. Diffusion calculations have been performed to estimate the magnitude of this escaping fraction of vacancies. The necessary cascade parameters for this calculation are obtained from MD simulation experiments. Almost 80% of vacancies retained in the vacancy-rich core of a 20 keV cascade are found to escape the cascade volume containing SIA clusters. We have taken this escape rate to be effective vacancy production rate in the medium and calculated the temporal evolution of SIA clusters, void size and swelling for fully annealed pure copper under neutron irradiation at 523 K. The general trends of the calculated swelling behaviour are found to be in agreement with experimental results. The present calculations provide further validity and support to the concept of production bias. (Author)

  5. 17 CFR 41.25 - Additional conditions for trading for security futures products.

    Science.gov (United States)

    2010-04-01

    ... security outstanding, the designated contract market or registered derivatives transaction execution... securities. (3) Notwithstanding paragraphs (b)(1) or (b)(2) of this section, if a derivatives clearing... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false Additional conditions for...

  6. Path dependence, initial conditions, and routines in organizations : The Toyota production system re-examined

    NARCIS (Netherlands)

    Dolfsma, W.A.; van Driel, H.

    2009-01-01

    Purpose - The purpose of this paper is to disentangle and elaborate on the constitutive elements of the concept of path dependence (initial conditions and lock-in) for a concerted and in-depth application to the study of organizational change. Design/methodology/approach - The approach takes the

  7. Sow body condition at weaning and reproduction performance in organic piglet production

    DEFF Research Database (Denmark)

    Kongsted, Anne Grete; Hermansen, John Erik

    2009-01-01

    that it is possible to avoid poor body condition at weaning even with a lactation length of seven weeks or more. No main effect of backfat at weaning on reproduction performance was found, but the probability of a successful reproduction after weaning tended to decrease with decreasing backfat for first parity sows...

  8. Technical bio-oils. Fundamentals - products - framework conditions; Technische Biooele. Grundlagen - Produkte - Rahmenbedingungen

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2012-03-27

    The Fachagentur Nachwachsende Rohstoffe e.V. (Guelzow-Pruezen, Federal Republic of Germany) and the Federal Ministry of Food, Agriculture and Consumer Protection (Bonn, Federal Republic of Germany) report on technical bio-oils. The contribution under consideration consists of the following chapters: Fundamentals, technical properties, applications, environmental aspects, legal framework conditions, funding measures; market of biological lubricants, economic operation with biological lubricants.

  9. Yeast production from cellulase hydrolyzed furfural industrial waste. II. Conditions for the cultivation of yeast

    Energy Technology Data Exchange (ETDEWEB)

    1977-01-01

    Three yeast strains, Candida AS 2-121, C. utilis AS 2-1180, and C. tropicalis AS 2-637 were selected as being capable of growing on cellulase-hydrolyzed furfural industrial waste. Cell mass yields with respect to C source were approximately 50%. Fermentation conditions are given.

  10. Specific characteristics of the aviary housing system affect plumage condition, mortality and production in laying hens.

    Science.gov (United States)

    Heerkens, Jasper L T; Delezie, Evelyne; Kempen, Ine; Zoons, Johan; Ampe, Bart; Rodenburg, T Bas; Tuyttens, Frank A M

    2015-09-01

    Feather pecking and high mortality levels are significant welfare problems in non-cage housing systems for laying hens. The aim of this study was to identify husbandry-related risk factors for feather damage, mortality, and egg laying performance in laying hens housed in the multi-tier non-cage housing systems known as aviaries. Factors tested included type of system flooring, degree of red mite infestation, and access to free-range areas. Information on housing characteristics, management, and performance in Belgian aviaries (N=47 flocks) were obtained from a questionnaire, farm records, and farm visits. Plumage condition and pecking wounds were scored in 50 randomly selected 60-week-old hens per flock. Associations between plumage condition, wounds, performance, mortality, and possible risk factors were investigated using a linear model with a stepwise model selection procedure. Many flocks exhibited a poor plumage condition and a high prevalence of wounds, with considerable variation between flocks. Better plumage condition was found in wire mesh aviaries (Pfeather cover had lower levels of mortality (Pnest perches. Wire mesh flooring in particular seems to have several health, welfare, and performance benefits in comparison to plastic slats, possibly related to decreased feather pecking, better hygiene, and fewer red mite infestations. This suggests that adjustments to the aviary housing design may further improve laying hen welfare and performance. © 2015 Poultry Science Association Inc.

  11. Storage condition of mulberry branches (Morus sp. in the survival, development and production of Bombyx mori L.

    Directory of Open Access Journals (Sweden)

    Antonio José Porto

    2012-02-01

    Full Text Available The study was carried with the objective of evaluate the survival, development and cocoons production of silkworm fed with mulberry leaves (Cultivar IZ 56/4 from branches stored in warehouse(24 hours or stored in the system of covering with wet cloth and immersion of bases in water, for a period of 72 hours. It was used a completely randomized design, with two treatments and six replications. Caterpillars fed with mulberry leaves from branches stored in the system of covering and immersion for 72 hours had conditions suitable for survival, development and production of cocoon, not differing from those who received leaves from branches stored in the warehouse.

  12. Formation and characterization of fission-product aerosols under postulated HTGR accident conditions

    International Nuclear Information System (INIS)

    Tang, I.N.; Munkelwitz, H.R.

    1982-07-01

    The paper presents the results of an experimental investigation on the formation mechanism and physical characterization of simulated nuclear aerosols that could likely be released during an HTGR core heat-up accident. Experiments were carried out in a high-temperature flow system consisting essentially of an inductively heated release source, a vapor deposition tube, and a filter assembly for collecting particulate matter. Simulated fission products Sr and Ba as oxides are separately impregnated in H451 graphite wafers and released at elevated temperatures into a dry helium flow. In the presence of graphite, the oxides are quantitatively reduced to metals, which subsequently vaporize at temperatures much lower than required for the oxides alone to vaporize in the absence of graphite. A substantial fraction of the released material is associated with particulate matter, which is collected on filters located downstream at ambient temperature. The release and transport of simulated fission product Ag as metal are also investigated

  13. Fission product release from high gap-inventory LWR fuel under LOCA conditions

    International Nuclear Information System (INIS)

    Lorenz, R.A.; Collins, J.L.; Osborne, M.F.; Malinauskas, A.P.

    1980-01-01

    Fission product release tests were performed with light water reactor (LWR) fuel rod segments containing large amounts of cesium and iodine in the pellet-to-cladding gap space in order to check the validity of the previously published Source Term Model for this type of fuel. The model describes the release of fission product cesium and iodine from LWR fuel rods for controlled loss-of-coolant accident (LOCA) transients in the temperature range 500 to 1200 0 C. The basis for the model was test data obtained with simulated fuel rods and commercial fuel irradiated to high burnup but containing relatively small amounts of cesium and iodine in the pellet-to-cladding gap space

  14. Apparatus for emulsion production in small scale and under controlled shear conditions

    DEFF Research Database (Denmark)

    Adler-Nissen, Jens; Mason, Sarah; Jacobsen, Charlotte

    2004-01-01

    In this article, a rotor-stator apparatus for the production of 5 g batches of emulsion is introduced. Special attention was paid to the design of the apparatus and its construction, ensuring close tolerances in all machined parts. The size of the dispersing gap was 500 µm. The need to prepare...... small quantities of homogeneous emulsion formulations containing costly ingredients formed the impetus for this work. We present a set of emulsion production experiments using a model mayonnaise recipe with a weight percentage of dispersed oil of 80%, and illustrate the effect of rotor speed...... on the average size and size distributions of the resulting oil droplets. These size distributions were within the same range as a commercial mayonnaise. The maximum shear rates and corresponding shear stresses existing in the apparatus at different rotational speeds were estimated. A stabilization time related...

  15. Study of conditions of production and characterization of noble metal micro-particles suspensions

    International Nuclear Information System (INIS)

    Malabre, Catherine

    1983-01-01

    As the production and identification of metal micro-particle suspensions are some aspects of issues related to nuclear fuel reprocessing, this research thesis reports the use of ruthenium, molybdenum, niobium, palladium and rhodium (fission metals) to generate such micro-particles. They are produced by erosion of two electrodes between which occurs an electric arc discharge in aqueous media. Different analytic methods are developed to determine the characteristics of so-produced colloidal solutions. A granulometry study is performed by transmission electronic microscopy, light quasi-elastic scattering, and turbidimetry associated to centrifugation. This has lead to the production of steady micro-particle suspensions which have been used in a first set of industrial trials [fr

  16. Analysis of displacement damage and defect production under cascade damage conditions

    DEFF Research Database (Denmark)

    Zinkle, S.J.; Singh, B.N.

    1993-01-01

    and residual defect production must be treated separately. An evaluation of experimental and computer defect production studies indicates that the overall fraction of defects surviving correlated annihilation in an energetic displacement cascade in copper decreases from about 30% of the Norgett......-Robinson-Torrens (NRT) calculated displacements at 4 K to about 10% of the NRT displacements at 300 K. Due to differences in the thermal stability of vacancy versus interstitial clusters, the fractions of freely migrating defects available for inducing microstructural changes at elevated temperatures may be higher...... for vacancies than for interstitials. The available evidence suggests that the fraction of freely migrating vacancies at temperatures relevant for void swelling in copper is greater than or similar to 5% of the calculated NRT displacements....

  17. Organisation of working time: Implications for productivity and working conditions. Overview Report

    OpenAIRE

    Goudswaard, A.; Dhondt, S.; Vergeer, R.; Oeij, P.; Leede, J. de; Adrichem, K. van C; Csizmadia, P.; Makó, C.; Illésy, M.; Tóth, A.

    2012-01-01

    Companies in search of improved productivity use a wide range of approaches, from financial incentives and skills upgrading and training to increased autonomy of individuals and teams. Working time flexibility has the added advantage in that it can benefit both workers and employers: it gives workers more control over their work–life balance and allows companies to adjust more easily to changing economic circumstances. Eurofound research studied five companies in manufacturing and retail in B...

  18. Deregulated electricity markets with thermal losses and production bounds: models and optimality conditions

    Czech Academy of Sciences Publication Activity Database

    Aussel, D.; Červinka, Michal; Marechal, M.

    2016-01-01

    Roč. 50, č. 1 (2016), s. 19-38 ISSN 0399-0559 R&D Projects: GA ČR GAP402/12/1309; GA ČR GA201/09/1957 Institutional support: RVO:67985556 Keywords : Deregulated electricity market * production bounds * mathematical program with complementarity constraints * M-stationarity * calmness Subject RIV: BA - General Mathematics Impact factor: 0.550, year: 2016

  19. Production of cultivars of Anthurium andraeanum in Acorizal-MT conditions

    Directory of Open Access Journals (Sweden)

    Carmen Lúcia Ferreira Fava

    2014-11-01

    cover with sugarcane bagasse. The cultivars Eidibel and Supremo were the most productive with 2,4 stalks/month/plant; the bigger spathe was obtained by the cultivar Brasão with 17,2 cm of width and 12,6 cm length; and the cultivar Sonata presents the bigger spadix with 7,6 cm. The seven cultivars of anthurium, as a cut flower, develop and bloom satisfactorily.

  20. Pot plant production, environmental conditions and energy consumption in insulated greenhouses

    Energy Technology Data Exchange (ETDEWEB)

    Bjerre, H.; Amsen, M.G. (Statens Planteavlsforsoeg, Havebrugscentret, Institut for Vaeksthuskulturer, Aarslev, Denmark)

    1984-01-01

    An energy experiment with 4 different types of greenhouses was carried out in the winter 1980-81 and 1981-82. Three of these greenhouses were insulated. The reference house was a single layer glasshouse with a mobile shading curtain, which was drawn at night. A comparison with the reference house showed the following energy savings for the insulated houses: Double glass 29-32%, double acryllic 39%, and thermal screens 22-24%. On average the air humidity was 80-86% RH in the double acryllic greenhouse and in the double glass house, whereas the levels was 5-10% lower in the 2 greenhouses with single glass. In spite of the high air humidity in the permanently insulated houses, no plant diseases occurred. The dry matter production of seven plant species was recorded in all greenhouses on the same date. Compared with the reference house 3 of the plant species showed a 5-10% higher production in the double acryllic greenhouse as well as the house with thermal screens. The remaining 4 plant species did not show any differences, between the 3 greenhouses. In the double glass house the production was considerably lower. To study the growth in detail, Tagetes plants were grown for 3-week periods during the winter in all houses. The aim of this study was to investigate whether the ratio between the growth in the 4 greenhouses was the same when periods of high light intensity were compared to periods with low light intensity. No characteristic changes with increasing light intensities could be observed between the different greenhouses. The differences between the greenhouses in time of production for the pot plants were generally small. The most remarkable difference in plant quality between the houses could be seen with Chrysanthemum and Kalanchoe. These 2 plant species were considerably less compact in the double acryllic greenhouse. Chrysanthemum was also less compact in the double glass house.

  1. Mental-Health Conditions, Barriers to Care, and Productivity Loss Among Officers in An Urban Police Department

    Science.gov (United States)

    Fox, Justin; Desai, Mayur M.; Britten, Karissa; Lucas, Georgina; Luneau, Renee; Rosenthal, Marjorie S.

    2014-01-01

    Background Police officers are frequently exposed to situations that can negatively impact their mental health. Methods We conducted this study of an urban police department to determine 1) the prevalence of post-traumatic stress disorder (PTSD), depression, and alcohol abuse; 2) patterns of and barriers to mental-health services utilization; and 3) the impact these conditions have on productivity loss. Results Among 150 officers, PTSD (24%), depression (9%), and alcohol abuse (19%) were common. Only 46.7% had ever sought mental-health services; the most commonly cited barriers to accessing services were concerns regarding confidentiality and the potential “negative career impact.” Officers with mental-health conditions had higher productivity loss (5.9% vs 3.4%, P police officers are common, and costly, yet most officers had never accessed mental-health services; many due to modifiable risk factors. PMID:23155671

  2. Melatonin enhances lipid production in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions via a multi-level mechanism.

    Science.gov (United States)

    Zhao, Yongteng; Li, Dafei; Xu, Jun-Wei; Zhao, Peng; Li, Tao; Ma, Huixian; Yu, Xuya

    2018-07-01

    In this study, melatonin (MT) promoted lipid accumulation in Monoraphidium sp. QLY-1 under nitrogen deficiency conditions. The lipid accumulation increased 1.22- and 1.36-fold compared with a nitrogen-starved medium and a normal BG-11 medium, respectively. The maximum lipid content was 51.38%. The reactive oxygen species (ROS) level in the presence of melatonin was lower than that in the control group, likely because of the high antioxidant activities. The application of melatonin upregulated the gibberellin acid (GA) production and rbcL and accD expression levels but downregulated the abscisic acid (ABA) content and pepc expression levels. These findings demonstrated that exogenous melatonin could further improve the lipid production in Monoraphidium sp. QLY-1 by regulating antioxidant systems, signalling molecules, and lipid biosynthesis-related gene expression under nitrogen deficiency conditions. Copyright © 2018 Elsevier Ltd. All rights reserved.

  3. Stimulation Of The Methane Production With The Use Of Changing Of The Rock Massif Physical Conditions

    Directory of Open Access Journals (Sweden)

    Baev Mikhail

    2017-01-01

    Full Text Available The commercial coalbed methane production success is majorly defined by the effectiveness of the use of special gas inflow stimulation methods. The necessity of using of such methods issubject to the aspects of searching and displacement of methane within the coal compound. Theanalysis of the ways of methane production stimulation from virgin coal formations is given. The description of the process of hydraulic fracturing (fracturing as the most common stimulation method during the commercial coalbed methane production as well as its major advantages are presented. The present work provides data about the initiated laboratory research of sands collected from Kemerovo region deposits for the purpose of finding of the most prospective samples by means of anchoring of fractures. The prospectivity and ability to implement the hydraulic fracturing with the use of locally available sands acting as proppants are shown. The influence of the strain-stress state of the rock massif on the alteration of permeability and the necessity of its extension study with respect to different technological features of hydraulic fracturing is shown

  4. Determination of Shelf Life for Butter and Cheese Products in Actual and Accelerated Conditions.

    Science.gov (United States)

    Park, Jung-Min; Shin, Jin-Ho; Bak, Da-Jeong; Kim, Na-Kyeong; Lim, Kwang-Sei; Yang, Cheul-Young; Kim, Jin-Man

    2014-01-01

    The aim of this study was to estimate the shelf life of butter and cheese products, with shelf life being a guide used to determine the storage period of food before deterioration. Butter and cheese samples stored at 10℃ and 15℃ had a shelf life of 221 d, while those stored at 25℃ and 35℃ had a shelf life of 109 d. Quality changes, including total cell count, coliform counts, Listeria monocytogenes counts, acid value, moisture content, pH, acidity and overall sensory evaluation, were monitored. In order to pass the overall sensory evaluation, a quality score of 5 points on a 9-point scale was required. For other quality criteria, legal quality limits were established based on the "Process Criteria and Ingredient Standard of Livestock Products" by the Animal, Plant and Fisheries Quarantine and Inspection Agency (Republic of Korea). The nonlegal quality limit was estimated by regression analysis between non-quality criteria (y) and overall sensory evaluation (x). The shelf life was estimated based on the number of days that the product passed the quality limit of the quality criteria. The shelf life of samples stored at 10℃, 15℃, 25℃ and 35℃ was 21.94, 17.18, 6.10 and 0.58 mon, respectively, for butter and 10.81, 9.47, 4.64 and 0.20 mon, respectively, for cheese.

  5. Aspergillus oryzae S2 alpha-amylase production under solid state fermentation: optimization of culture conditions.

    Science.gov (United States)

    Sahnoun, Mouna; Kriaa, Mouna; Elgharbi, Fatma; Ayadi, Dorra-Zouari; Bejar, Samir; Kammoun, Radhouane

    2015-04-01

    Aspergillus oryzae S2 was assayed for alpha-amylase production under solid state fermentation (SSF). In addition to AmyA and AmyB already produced in monitored submerged culture, the strain was noted to produce new AmyB oligomeric forms, in particular a dominant tetrameric form named AmyC. The latter was purified to homogeneity through fractional acetone precipitation and size exclusion chromatography. SDS-PAGE and native PAGE analyses revealed that, purified AmyC was an approximately 172 kDa tetramer of four 42 kDa subunits. AmyC was also noted to display the same NH2-terminal amino acid sequence residues and approximately the same physico-chemical properties of AmyA and AmyB, to exhibit maximum activity at pH 5.6 and 60 °C, and to produce maltose and maltotriose as major starch hydrolysis end-products. Soyabean meal was the best substitute to yeast extract compared to fish powder waste and wheat gluten waste. AmyC production was optimized under SSF using statistical design methodology. Moisture content of 76.25%, C/N substrate ratio of 0.62, and inoculum size of 10(6.87) spores allowed maximum activity of 22118.34 U/g of dried substrate, which was 33 times higher than the one obtained before the application of the central composite design (CCD). Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Ethanol production from rape straw by a two-stage pretreatment under mild conditions.

    Science.gov (United States)

    Romero, Inmaculada; López-Linares, Juan C; Delgado, Yaimé; Cara, Cristóbal; Castro, Eulogio

    2015-08-01

    The growing interest on rape oil as raw material for biodiesel production has resulted in an increasing availability of rape straw, an agricultural residue that is an attractive renewable source for the production of second-generation bioethanol. Pretreatment is one of the key steps in such a conversion process. In this work, a sequential two-stage pretreatment with dilute sulfuric acid (130 °C, 60 min, 2% w/v H2SO4) followed by H2O2 (1-5% w/v) in alkaline medium (NaOH) at low temperature (60, 90 °C) and at different pretreatment times (30-90 min) was investigated. The first-acid stage allows the solubilisation of hemicellulose fraction into fermentable sugars. The second-alkaline peroxide stage allows the delignification of the solid material whilst the cellulose remaining in rape straw turned highly digestible by cellulases. Simultaneous saccharification and fermentation with 15% (w/v) delignified substrate at 90 °C, 5% H2O2 for 60 min, led to a maximum ethanol production of 53 g/L and a yield of 85% of the theoretical.

  7. Optimization of ultrasonic emulsification conditions for the production of orange peel essential oil nanoemulsions.

    Science.gov (United States)

    Hashtjin, Adel Mirmajidi; Abbasi, Soleiman

    2015-05-01

    The aim of the present study was to investigate the influence of emulsifying conditions on some physical and rheological properties of orange peel essential oil (OPEO) in water nanoemulsions. In this regard, using the response surface methodology, the influence of ultrasonication conditions including sonication amplitude (70-100 %), sonication time (90-150 s) and process temperature (5-45 °C) on the mean droplets diameter (Z-average value), polydispersity index (PDI), and viscosity of the OPEO nanoemulsions was evaluated. In addition, the flow behavior and stability of selected nanoemulsions was evaluated during storage (up to 3 months) at different temperatures (5, 25 and 45 °C). Based on the results of the optimization, the optimum conditions for producing OPEO nanoemulsions (Z-average value 18.16 nm) were determined as 94 % (sonication amplitude), 138 s (sonication time) and 37 °C (process temperature). Moreover, analysis of variance (ANOVA) showed high coefficients of determination values (R (2) > 0.95) for the response surface models of the energy input and Z-average. In addition, the flow behavior of produced nanoemulsions was Newtonian, and the effect of time and storage temperature as well as their interactions on the Z-average value was highly significant (P < 0.0001).

  8. The improved heat integration of cement production under limited process conditions: A case study for Croatia

    International Nuclear Information System (INIS)

    Boldyryev, Stanislav; Mikulčić, Hrvoje; Mohorović, Zoran; Vujanović, Milan; Krajačić, Goran; Duić, Neven

    2016-01-01

    Given that cement is the most widely used material for housing and modern infrastructure needs, this paper analyses the energy efficiency of the cement manufacturing processes for a particular cement plant. The cement industry is one of the largest consumers of carbon-containing primary energy sources and one of the primary polluters of the environment, emitting approximately 5% of global pollution. Energy consumption represents the largest part of the production cost for cement factories and has a significant influence on product prices. Given that it is realised in modern society that infrastructural projects lead to a higher level of economy and sustainability for countries, reducing the production cost in the cement industry is a very important problem. The authors analysed the energy consumption of a particular cement factory in Croatia to determine the minimum energy targets of production and proposed pathways to improve energy efficiency. The Process Integration approach was used in this study. Nevertheless, the features of the cement factory forced the research to update its methodological steps to propose real pathways for a retrofit project with the aim of achieving the optimal minimum temperature difference between process streams. There are various streams, including those that contain solid particles, gas and air streams, and streams, that should be cooled down rapidly; these facts become more complicated by the special construction of the process equipment, which causes heat transfer between some streams to be impossible. The main objective of this paper is to determine the potential of real energy savings and propose a solution for a new concept of heat exchanger network (HEN) that avoids the process traps and provides a feasible retrofit. The maximum heat recovery of that production of a particular type of cement was determined and improved when a HEN was built. The authors conclude that the energy consumption of the cement factory can be reduced by

  9. Evaluation of Carcass Production of PO Cattle Based on Heart Girth Measurement, Body Condition Score and Slaughter Weight

    OpenAIRE

    Haryoko, I; Suparman, P

    2009-01-01

    The objective of study was to evaluate of carcass production of PO beef cattle based on measurement of heart girth, body condition score (BCS), and slaughter weight. It was conducted in the slaughtering house at Mersi Purwokerto city. The materials for this study were 60 heads of male PO breed cattle. Simple random sampling was used for taking samples. Data was analyzed by using multiple regression equation to determine the effects of heart girth, BCS, and slaughter weight on carcass weig...

  10. Biodiesel production from various oils under supercritical fluid conditions by Candida antartica lipase B using a stepwise reaction method.

    Science.gov (United States)

    Lee, Jong Ho; Kwon, Cheong Hoon; Kang, Jeong Won; Park, Chulhwan; Tae, Bumseok; Kim, Seung Wook

    2009-05-01

    In this study, we evaluate the effects of various reaction factors, including pressure, temperature, agitation speed, enzyme concentration, and water content to increase biodiesel production. In addition, biodiesel was produced from various oils to establish the optimal enzymatic process of biodiesel production. Optimal conditions were determined to be as follows: pressure 130 bar, temperature 45 degrees C, agitation speed 200 rpm, enzyme concentration 20%, and water contents 10%. Among the various oils used for production, olive oil showed the highest yield (65.18%) upon transesterification. However, when biodiesel was produced using a batch system, biodiesel conversion yield was not increased over 65%; therefore, a stepwise reaction was conducted to increase biodiesel production. When a reaction medium with an initial concentration of methanol of 60 mmol was used and adjusted to maintain this concentration of methanol every 1.5 h during biodiesel production, the conversion yield of biodiesel was 98.92% at 6 h. Finally, reusability was evaluated using immobilized lipase to determine if this method was applicable for industrial biodiesel production. When biodiesel was produced repeatedly, the conversion rate was maintained at over 85% after eight reuses.

  11. DEVELOPMENT AND APPLICATION OF THE KEY TECHNOLOGIES FOR THE QUALITY CONTROL AND INSPECTION OF NATIONAL GEOGRAPHICAL CONDITIONS SURVEY PRODUCTS

    Directory of Open Access Journals (Sweden)

    Y. Zhao

    2018-04-01

    Full Text Available The First National Geographical Condition Survey is a predecessor task to dynamically master basic situations of the nature, ecology and human activities on the earth’s surface and it is the brand-new mapping geographic information engineering. In order to ensure comprehensive, real and accurate survey results and achieve the quality management target which the qualified rate is 100 % and the yield is more than 80 %, it is necessary to carry out the quality control and result inspection for national geographical conditions survey on a national scale. To ensure that achievement quality meets quality target requirements, this paper develops the key technology method of “five-in-one” quality control that is constituted by “quality control system of national geographical condition survey, quality inspection technology system, quality evaluation system, quality inspection information management system and national linked quality control institutions” by aiming at large scale, wide coverage range, more undertaking units, more management levels, technical updating, more production process and obvious regional differences in the national geographical condition survey and combining with novel achievement manifestation, complicated dependency, more special reference data, and large data size. This project fully considering the domestic and foreign related research results and production practice experience, combined with the technology development and the needs of the production, it stipulates the inspection methods and technical requirements of each stage in the quality inspection of the geographical condition survey results, and extends the traditional inspection and acceptance technology, and solves the key technologies that are badly needed in the first national geographic survey.

  12. A Status of Art-Report on the Fission Products Behavior Released from Spent Fuel at High Temperature Conditions

    International Nuclear Information System (INIS)

    Park, Geun Il; Kim, J. H.; Lee, J. W.

    2003-04-01

    The experiments on the fission products release behavior from spent fuel at high temperature assuming reactor accident conditions have been carried out at Oak Ridge Nation Laboratory of USA in HI/VI tests, CEA of France in HEVA/VERCOS tests, AEA of England and CRNL of Canada in HOX test. The VEGA program to study the fission product release behavior from LWR irradiated fuel was recently initiated at JAERI. The key parameter affecting the fission product(FP) release behavior is temperature. In addition, other parameters such as fuel oxidation, burnup, pre-transient conditions are found to affect the FP releases considerably in the earlier tests. The atmosphere conditions such as oxidizing atmosphere (steam or air) or reducing atmosphere (hydrogen) can cause significant change of FPs release and transport behavior due to chemical forms of the reactive FPs which is dependent on the oxidation potential. The effect of fuel burnup on the Kr-85 or Cs-137 release showed that the release rates of these radionuclides increased with the increase of burnup, meaning that release rates are dominated by the atomic diffusions in the grains and they are primarily a function of temperature. However, the data on FPs release behavior using higher burnups above 50,000 MWD/MTU are not so many reported up to now. This report summarizes the test results of FPs release behavior in reactor accident conditions produced from other countries mentioned above. This review and analysis on earlier studies would be useful for predicting the release characteristics of FPs from domestic spent fuel. The release rates of fission gas or FPs from spent fuel at high temperature conditions during fabrication process of dry recycling fuel were also analyzed using many data obtained from earlier tests

  13. Stability of Anthocyanins and Their Degradation Products from Cabernet Sauvignon Red Wine under Gastrointestinal pH and Temperature Conditions.

    Science.gov (United States)

    Yang, Ping; Yuan, Chunlong; Wang, Hua; Han, Fuliang; Liu, Yangjie; Wang, Lin; Liu, Yang

    2018-02-07

    This study investigated the stability of wine anthocyanins under simulated gastrointestinal pH and temperature conditions, and further studied the evolution of anthocyanin degradation products through simulated digestive conditions. The aim of this study was to investigate the relation between anthocyanins' structure and their digestive stability. Results showed that a total of 22 anthocyanins were identified in wine and most of these anthocyanins remained stable under simulated gastric digestion process. However, a dramatic concentration decrease happened to these anthocyanins during simulated intestinal digestion. The stability of anthocyanins in digestive process appeared to be related to their structure. The methoxy group in the B-ring enhanced the stability of anthocyanins, whereas hydroxyl group resulted in a reduction of their stability. Acylation decreased the stability of malvidin 3- O -glucoside. Pyruvic acid conjugation enhanced the structural stability of pyranoanthocyanins, whereas acetaldehyde attachment weakened their stability. A commercial malvidin 3- O -glucoside standard was used to investigate anthocyanin degradation products under simulated digestion process, and syringic acid, protocatechuic acid and vanillic acid were confirmed to be the degradation products via anthocyanin chalcone conversion path. Gallic acid, protocatechuic acid, vanillic acid, syringic acid, and p -coumaric acid in wine experienced a significant concentration decrease during digestion process. However, wine model solution revealed that phenolic acids remained stable under gastrointestinal conditions, except gallic acid.

  14. Stability of Anthocyanins and Their Degradation Products from Cabernet Sauvignon Red Wine under Gastrointestinal pH and Temperature Conditions

    Directory of Open Access Journals (Sweden)

    Ping Yang

    2018-02-01

    Full Text Available This study investigated the stability of wine anthocyanins under simulated gastrointestinal pH and temperature conditions, and further studied the evolution of anthocyanin degradation products through simulated digestive conditions. The aim of this study was to investigate the relation between anthocyanins’ structure and their digestive stability. Results showed that a total of 22 anthocyanins were identified in wine and most of these anthocyanins remained stable under simulated gastric digestion process. However, a dramatic concentration decrease happened to these anthocyanins during simulated intestinal digestion. The stability of anthocyanins in digestive process appeared to be related to their structure. The methoxy group in the B-ring enhanced the stability of anthocyanins, whereas hydroxyl group resulted in a reduction of their stability. Acylation decreased the stability of malvidin 3-O-glucoside. Pyruvic acid conjugation enhanced the structural stability of pyranoanthocyanins, whereas acetaldehyde attachment weakened their stability. A commercial malvidin 3-O-glucoside standard was used to investigate anthocyanin degradation products under simulated digestion process, and syringic acid, protocatechuic acid and vanillic acid were confirmed to be the degradation products via anthocyanin chalcone conversion path. Gallic acid, protocatechuic acid, vanillic acid, syringic acid, and p-coumaric acid in wine experienced a significant concentration decrease during digestion process. However, wine model solution revealed that phenolic acids remained stable under gastrointestinal conditions, except gallic acid.

  15. Trends, productivity losses, and associated medical conditions among toxoplasmosis deaths in the United States, 2000-2010.

    Science.gov (United States)

    Cummings, Patricia L; Kuo, Tony; Javanbakht, Marjan; Sorvillo, Frank

    2014-11-01

    Few studies have quantified toxoplasmosis mortality, associated medical conditions, and productivity losses in the United States. We examined national multiple cause of death data and estimated productivity losses caused by toxoplasmosis during 2000-2010. A matched case-control analysis examined associations between comorbid medical conditions and toxoplasmosis deaths. In total, 789 toxoplasmosis deaths were identified during the 11-year study period. Blacks and Hispanics had the highest toxoplasmosis mortality compared with whites. Several medical conditions were associated with toxoplasmosis deaths, including human immunodeficiency virus (HIV), lymphoma, leukemia, and connective tissue disorders. The number of toxoplasmosis deaths with an HIV codiagnosis declined from 2000 to 2010; the numbers without such a codiagnosis remained static. Cumulative disease-related productivity losses for the 11-year period were nearly $815 million. Although toxoplasmosis mortality has declined in the last decade, the infection remains costly and is an important cause of preventable death among non-HIV subgroups. © The American Society of Tropical Medicine and Hygiene.

  16. β-cell serotonin production is associated with female sex, old age, and diabetes-free condition.

    Science.gov (United States)

    Kim, Yeong Gi; Moon, Joon Ho; Kim, Kyuho; Kim, Hyeongseok; Kim, Juok; Jeong, Ji-Seon; Lee, Junguee; Kang, Shinae; Park, Joon Seong; Kim, Hail

    2017-11-25

    Serotonin is known to be present in pancreatic β-cells and to play several physiological roles, including insulin secretion, β-cell proliferation, and paracrine inhibition of α-cells. However, the serotonin production of different cell lines and islets has not been compared based on age, sex, and diabetes related conditions. Here, we directly compared the serotonin concentrations in βTC and MIN6 cell lines, as well as in islets from mice using ultra-performance liquid chromatography tandem mass spectrometry. The average serotonin concentration was 5-10 ng/mg protein in the islets of male and non-pregnant female mice. The serotonin level was higher in females than males at 8 weeks, although there was no difference at 1 year. Furthermore, we observed serotonin by immunofluorescence staining in the pancreatic tissues of mice and human. Serotonin was detected by immunofluorescence staining in a portion of β-cells from islets of old female mice, but not of male or young female mice. A similar pattern was observed in human pancreas as well. In humans, serotonin production in β-cells was associated with a diabetes-free condition. Thus, serotonin production in β-cells was associated with old age, female sex, and diabetes-free condition. Copyright © 2017 Elsevier Inc. All rights reserved.

  17. Prevention of melanin formation during aryl alcohol oxidase production under growth-limited conditions using an Aspergillus nidulans cell factory.

    Science.gov (United States)

    Pardo-Planas, Oscar; Prade, Rolf A; Müller, Michael; Atiyeh, Hasan K; Wilkins, Mark R

    2017-11-01

    An Aspergillus nidulans cell factory was genetically engineered to produce an aryl alcohol oxidase (AAO). The cell factory initiated production of melanin when growth-limited conditions were established using stationary plates and shaken flasks. This phenomenon was more pronounced when the strain was cultured in a trickle bed reactor (TBR). This study investigated different approaches to reduce melanin formation in fungal mycelia and liquid medium in order to increase the enzyme production yield. Removal of copper from the medium recipe reduced melanin formation in agar cultures and increased enzyme activities by 48% in agitated liquid cultures. Copper has been reported as a key element for tyrosinase, an enzyme responsible for melanin production. Ascorbic acid (0.44g/L) stopped melanin accumulation, did not affect growth parameters and resulted in AAO activity that was more than two-fold greater than a control treatment with no ascorbic acid. Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Service oriented product innovation for improved environmental performance. An an exploratory case study of the air conditioning and cooling sector

    Energy Technology Data Exchange (ETDEWEB)

    Cook, M.; Maggs, H.; Neame, C.; Lemon, M. [The School of Applied Sciences, Cranfield University, Cranfield, Bedfordshire (United Kingdom)

    2006-09-15

    The need to improve the environmental performance of production and consumption practices within advanced industrialised nations is widely accepted. Finding ways to satisfy demand using far fewer resources is central to research in this field. For many, the trajectories of service orientated products are thought to provide an opportunity to address this need and anticipate futures in which economic growth is de-coupled from resource use. This paper presents the findings of exploratory research in the air-conditioning and cooling sector, which sought to understand how these benefits might be realised. It suggests that these benefits will not arise as a result of economic restructuring but rather that a deeper understanding of the process of service innovation, which underpins trajectories of service orientated products, is required to develop effective policy.

  19. Conditions of prospecting, development, production, and supply of oil and natural gas in Cameroun, Congo, and Ivory Coast

    Energy Technology Data Exchange (ETDEWEB)

    Shirakami, Yoshimasa; Norisugi, Yoichi; Miyake, Keiji

    1988-08-01

    This paper reports the conditions of national affairs, oil industry, and prospecting, development, production of oil and natural gas in Ivory Coast in western Africa. All of oil and natural gas are produced in Cretaceous and Tertiary sedimentary basins on the continental margin off the coast of Ivory Coast and Ghana. In 1970 the first oil field (Belier) was discovered by the test boring drilled under the sea, and in 1980 Espoir Oil Field was discovered. No further new oil field, however, has been discovered since then. The total production until 1987, amounts to 45.55 million bbl and the minable reserve is estimated about 84 million bbl. The production reached the maximum 1984, and thereafter has gone down. Few plans of prospecting are carried out recently. (10 figs, 2 tabs)

  20. Fermentation Conditions and Media Optimization for Isocitric Acid Production from Ethanol by Yarrowia lipolytica

    Directory of Open Access Journals (Sweden)

    Svetlana V. Kamzolova

    2018-01-01

    Full Text Available Isocitric acid exists in the form of four stereoisomers, of which only the threo-Ds-form (ICA is a natural active compound, an intermediate of Krebs cycle, and suitable for nutritional and pharmaceutical use. In this paper, we propose a method for ICA production from ethanol by yeast Yarrowia lipolytica. The effects of temperature, pH of the medium, and aeration on the growth of the producer Y. lipolytica VKM Y-2373 and synthesis of ICA were studied. An optimal fermentation regime, which ensures a good growth of the producer and directed synthesis of the target product, was determined. The producer is advised to carry out cultivation at 29°C and various pH of the medium and the oxygen concentration (pH 5 and pO2 20–25% (of saturation during the growth period and pH 6 and pO2 50–55% (of saturation during the acid formation on a nutrient medium containing an increased content of zinc (0.6 mg/L, iron (1.2 mg/L, and 30 mM itaconic acid (inhibitor of isocitrate lyase—the key enzyme of ICA metabolism should also be introduced into the nutrition medium. Such fermentation production mode provides 90.5 g/L ICA with process selectivity of 80%, mass yield (YICA of 0.77 g/g, and energy yield (ηICA of 0.278 g/g.

  1. Effect of cultural conditions on antrodin C production by basidiomycete Antrodia camphorata in solid-state fermentation.

    Science.gov (United States)

    Xia, Yongjun; Wang, Yuanlong; Zhang, Bobo; Xu, Ganrong; Ai, Lianzhong

    2014-01-01

    Antrodia camphorata is a medicinal fungus and antrodin C is one of the main bioactive components of A. camphorata in the submerged fermentation (SmF). To optimize the culture conditions, the factors influencing the production of antrodin C by A. camphorata under solid-state fermentation (SSF) were investigated in this study. Different solid substrates and external nitrogen sources were tested for their efficiency in producing antrodin C. The response surface methodology was applied to evaluate the influence of several variables, namely, the concentrations of soybean meal, initial moisture content, and inoculum density on antrodin C production in solid-state fermentation. The experimental results show that the optimum fermentation medium for antrodin C production by A. camphorata was composed of 0.578 g soybean meal, 0.05 g Na2 HPO4 , 0.05 g MgSO4 for 100 g rice, with 51.83% initial moisture content, 22 day culture time, 28 °C culture temperature, and 35.54% inoculum density. At optimized conditions, 6,617.36 ± 92.71 mg kg(-1) yield of antrodin C was achieved. Solid-state fermentation is one good cultural method to improve the production of antrodin C by A. camphorata. © 2014 International Union of Biochemistry and Molecular Biology, Inc.

  2. Comparative Analysis of Culture Conditions for the Optimization of Carotenoid Production in Several Strains of the Picoeukaryote Ostreococcus

    Directory of Open Access Journals (Sweden)

    Jean-Baptiste Guyon

    2018-02-01

    Full Text Available Microalgae are promising sources for the sustainable production of compounds of interest for biotechnologies. Compared to higher plants, microalgae have a faster growth rate and can be grown in industrial photobioreactors. The microalgae biomass contains specific metabolites of high added value for biotechnology such as lipids, polysaccharides or carotenoid pigments. Studying carotenogenesis is important for deciphering the mechanisms of adaptation to stress tolerance as well as for biotechnological production. In recent years, the picoeukaryote Ostreococcus tauri has emerged as a model organism thanks to the development of powerful genetic tools. Several strains of Ostreococcus isolated from different environments have been characterized with respect to light response or iron requirement. We have compared the carotenoid contents and growth rates of strains of Ostreococcus (OTTH595, RCC802 and RCC809 under a wide range of light, salinity and temperature conditions. Carotenoid profiles and productivities varied in a strain-specific and stress-dependent manner. Our results also illustrate that phylogenetically related microalgal strains originating from different ecological niches present specific interests for the production of specific molecules under controlled culture conditions.

  3. Directions of development for areas with unfavourable conditions for agricultural production, an example of the podlaskie voivodeship (Poland

    Directory of Open Access Journals (Sweden)

    Kazimierz Niewiadomski

    2012-01-01

    Full Text Available This paper is dedicated to the directions of development of rural areas, primarily agricultural areas of the Podlaskie voivodeship, which in light of current criteria has been qualified almost entirely as problem area (ONW. Results presented are culmination of research related to issues of problem areas primarily in the area of the Podlaskie voivodeship by the author of the herewith paper during last few years. In conclusion to the results of the research it can be said that the primary direction of the development of rural areas in the Podlaskie voivodeship will be modern, large-scale farms able to adjust to current conditions, in particular environmental and soil conditions. In problem areas these will be mostly farms focusing on production of cattle and milk, developing production based on very high share of grassland areas. Complementary role in relation to conventional agriculture will be fulfilled by farms developing organic farming and agro-touristic farms. More intensive development of conventional tourism in rural areas can be expected once new tourism products and services have been developed. Assessment of the current economic development parameters of the Podlaskie voivodeship does not point at the convergence with other regions and possibility of decreasing the distance between the Podlaskie voivodeship and average results for Poland in the near future. However, some positive trends in terms of convergence with other regions in Poland can be observed in agriculture, primarily due to relatively good results in production of cattle and milk.

  4. Adverse weather conditions for European wheat production will become more frequent with climate change

    DEFF Research Database (Denmark)

    Trnka, Miroslav; Rötter, Reimund P.; Ruiz-Ramos, Margarita

    2014-01-01

    events that might significantly affect wheat yield in Europe. For this purpose we analysed changes in the frequency of the occurrence of 11 adverse weather events. Using climate scenarios based on the most recent ensemble of climate models and greenhouse gases emission estimates, we assessed...... crop failure across Europe. This study provides essential information for developing adaptation strategies.......Europe is the largest producer of wheat, the second most widely grown cereal crop after rice. The increased occurrence and magnitude of adverse and extreme agroclimatic events are considered a major threat for wheat production. We present an analysis that accounts for a range of adverse weather...

  5. Technological indicators of operation of the rotating-hearth furnace in conditions of discontinued production

    Directory of Open Access Journals (Sweden)

    Lazić, L.

    2008-04-01

    Full Text Available Quality heating of the steel charge to be rolled into seamless tubes in the rotating-hearth furnace requires continuous operation of the pilger mill. Interruption of the charging schedule leads to impaired charge heating process. Prolonged heating time causes a rise in the charge temperature above the limit values, and that in turn brings to a larger quantity of scale formed on the charge surface. Final result is loss of metal and overheating of the charge accompanied by increased fuel consumption and lower furnace productivity.

  6. PATTERN FORMATION OF BUSINESS CONDITIONS IN DOMESTIC MARKET OF CROP PRODUCTION

    Directory of Open Access Journals (Sweden)

    Svitlana Strapchuk

    2016-11-01

    Full Text Available The purpose of the article is to detect crop production on agricultural enterprises clusters at a price by defining their amplitude of price fluctuations. Methodology. The study is based on grouping of statistical data from agricultural enterprises using cluster analysis, followed by reliability evaluation of pre-selected clusters by t-test and charting the scope by the selected index. Cluster analysis of agricultural enterprises in Ukraine has been conducted using "STATISTICA" program. Distance between clusters was calculated as the Euclidean distance. The object of the study was data on the prices for agricultural enterprises by regions of Ukraine in 2013. As a result, an appropriate number of groups according to the produce types in the regions of Ukraine, plane in market prices, have been determined. The process of consistent combination of objects in clusters is shown in the graphs as agglomerative clustering dendrogram of the regions of Ukraine for such products as wheat, grain corn and sunflower seeds. In general, there have been examined: 7311 businesses growing wheat, 5034 – growing corn and 6124 companies growing sunflower. Results. During a year-long study of price fluctuations in agricultural enterprises within regions of Ukraine similarities in nature of absolute and relative changes in the formed clusters were established. Four clusters on wheat, five clusters on corn, three clusters on sunflower seeds have been allocated during the study. The study of the selected groups confirms significant differences between them and allows the sectors and enterprises of the cluster with high variability of prices to build their own marketing strategy based on the position of expectations and search for sale options according to the most favorable price. Practical value. The established differences on the selected clusters make it possible to forecast the price situation in various regions of Ukraine in terms of its differences from average

  7. Biological iron oxidation by Gallionella spp. in drinking water production under fully aerated conditions.

    Science.gov (United States)

    de Vet, W W J M; Dinkla, I J T; Rietveld, L C; van Loosdrecht, M C M

    2011-11-01

    Iron oxidation under neutral conditions (pH 6.5-8) may be a homo- or heterogeneous chemically- or a biologically-mediated process. The chemical oxidation is supposed to outpace the biological process under slightly alkaline conditions (pH 7-8). The iron oxidation kinetics and growth of Gallionella spp. - obligatory chemolithotrophic iron oxidizers - were assessed in natural, organic carbon-containing water, in continuous lab-scale reactors and full-scale groundwater trickling filters in the Netherlands. From Gallionella cell numbers determined by qPCR, balances were made for all systems. The homogeneous chemical iron oxidation occurred in accordance with the literature, but was retarded by a low water temperature (13 °C). The contribution of the heterogeneous chemical oxidation was, despite the presence of freshly formed iron oxyhydroxides, much lower than in previous studies in ultrapure water. This could be caused by the adsorption of natural organic matter (NOM) on the iron oxide surfaces. In the oxygen-saturated natural water with a pH ranging from 6.5 to 7.7, Gallionella spp. grew uninhibited and biological iron oxidation was an important, and probably the dominant, process. Gallionella growth was not even inhibited in a full-scale filter after plate aeration. From this we conclude that Gallionella spp. can grow under neutral pH and fully aerated conditions when the chemical iron oxidation is retarded by low water temperature and inhibition of the autocatalytic iron oxidation. Copyright © 2011 Elsevier Ltd. All rights reserved.

  8. Degradation of materials under conditions of thermochemical cycles for hydrogen production - part III

    International Nuclear Information System (INIS)

    Klimas, S.J.; Searle, H.; Guerout, F.

    2011-01-01

    A capsule method was employed to screen a number of materials for degradation under selected conditions of the sulphur-iodine (SI) and the copper-chlorine (Cu-Cl) thermochemical cycles. A summary of the results of an experimental investigation is given. The recommendations for the selection of the materials required for the construction of the electrolyser subsystem of the copper chlorine hybrid cycle are presented and discussed with the associated rationale. Some remaining uncertainties are illustrated on the basis of the experimental evidence gathered. (author)

  9. Seed production of woody plants in conditions of environment pollution by metallurgical industry emissions

    Directory of Open Access Journals (Sweden)

    Z. V. Gritzay

    2011-10-01

    Full Text Available The influence of environment pollution by metallurgical industry emissions on woody plants bearing parameters was examined. The results obtained show the decrease of bearing rate, diminution of seeds, fruits and seed cells sizes in woody plants affected by technogenic emissions. Attenuation of the 1000 seeds’ weight was established. Incresing the amount of fruits with development deviations was ascertained. It was found aplasia and abnormal form of the samara fruit of ash and ailanthus trees, arcuation and narrowing of some parts of the catalpa fruitcases. Practical recommendations on using seeds’ sensitive parameters in biomonitoring of woody phytocenoses under technogenic stressful conditions are proposed.

  10. Influence of mammal fossorial activity on the soil fermentative activity in conditions of metallurgical production

    Directory of Open Access Journals (Sweden)

    S. M. Kirienko

    2010-09-01

    Full Text Available Effect of mammal fossorial activity as an ecological factor of the soil genesis intensification is studied. Enzymatic activity of soil as its ability to demonstrate a catalytic effect for various compounds transformation is examined. Variability of soil urease activity in technogenic conditions with the participation of animals is shown. The positive influence of animals’ activity on the catalitic ability of the investigated soils was determined. The statistically significant characteristics which have an influence on the urease activity in soil are found out.

  11. Development of volatile compounds during storage of different skin care products at various conditions

    DEFF Research Database (Denmark)

    Horn, A. F.; Thomsen, Birgitte Raagaard; Hyldig, Grethe

    a storage experiment on three cleansing milks stored between 14 and 84 days, under different conditions. The samples were exposed to heat (20°C, 40°C and 50°C), light (samples at 20°C) and iron (samples at 40°C). Samples were analysed for their development of volatile compounds by dynamic headspace gas...... chromatography-mass spectrometry and peroxide value, and compared to samples stored at 2°C in the dark. In addition, sensory analyses were carried out to assess the off-odours developed in the samples....

  12. Life cycle impacts of ethanol production from spruce wood chips under high-gravity conditions.

    Science.gov (United States)

    Janssen, Matty; Xiros, Charilaos; Tillman, Anne-Marie

    2016-01-01

    Development of more sustainable biofuel production processes is ongoing, and technology to run these processes at a high dry matter content, also called high-gravity conditions, is one option. This paper presents the results of a life cycle assessment (LCA) of such a technology currently in development for the production of bio-ethanol from spruce wood chips. The cradle-to-gate LCA used lab results from a set of 30 experiments (or process configurations) in which the main process variable was the detoxification strategy applied to the pretreated feedstock material. The results of the assessment show that a process configuration, in which washing of the pretreated slurry is the detoxification strategy, leads to the lowest environmental impact of the process. Enzyme production and use are the main contributors to the environmental impact in all process configurations, and strategies to significantly reduce this contribution are enzyme recycling and on-site enzyme production. Furthermore, a strong linear correlation between the ethanol yield of a configuration and its environmental impact is demonstrated, and the selected environmental impacts show a very strong cross-correlation ([Formula: see text] in all cases) which may be used to reduce the number of impact categories considered from four to one (in this case, global warming potential). Lastly, a comparison with results of an LCA of ethanol production under high-gravity conditions using wheat straw shows that the environmental performance does not significantly differ when using spruce wood chips. For this comparison, it is shown that eutrophication potential also needs to be considered due to the fertilizer use in wheat cultivation. The LCA points out the environmental hotspots in the ethanol production process, and thus provides input to the further development of the high-gravity technology. Reducing the number of impact categories based only on cross-correlations should be done with caution. Knowledge of the

  13. GERDA phase II detectors: Behind the production and characterisation at low background conditions

    Energy Technology Data Exchange (ETDEWEB)

    Maneschg, Werner [Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg (Germany); Collaboration: GERDA Collaboration; and others

    2013-08-08

    The low background GERmanium Detector Array (GERDA) at Laboratori Nazionali del Gran Sasso (LNGS) is designed to search for the rare neutrinoless double beta decay (0νββ) in {sup 76}Ge. Bare germanium diodes are operated in liquid argon which is used as coolant, as passive and soon active as well shield against external radiation. Currently, Phase I of the experiment is running using ∼15 kg of co-axial High Purity Germanium diodes. In order to increase the sensitivity of the experiment 30 Broad Energy Germanium (BEGe) diodes will be added within 2013. This presentation reviews the production chain of the new BEGe detectors from isotopic enrichment to diode production and testing. As demonstrated all steps were carefully planned in order to minimize the exposure of the enriched germanium to cosmic radiation. Following this premise, acceptance and characterisation measurement of the newly produced diodes have been performed within the HEROICA project in the Belgian underground laboratory HADES close to the diode manufacturer. The test program and the results from a subset of the recently terminated GERDA Phase II BEGe survey will be presented.

  14. Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02 under different nutrient conditions

    Directory of Open Access Journals (Sweden)

    Stella Bortoli

    Full Text Available Cyanobacteria are prokaryotic and photosynthetic organisms, which can produce a wide range of bioactive compounds with different properties; including a variety of toxic compounds, also known as cyanotoxins. In this work, we describe the isolation of seven cyanobacterial strains from two reservoirs in São Paulo State, Brazil. Seven different chemical variants of microcystins (MC-RR, MC-LR, MC-YR, MC-LF, MC-LW, and two demethylated variants, dm-MC-RR and dm-MC-LR were detected in three of the ten isolated strains. One particular Microcystis aeruginosa strain (LTPNA 02 was chosen to evaluate its growth by cell count, and its toxin production under seven different nutritional regimes. We observed different growth behaviors in the logarithmic growth period for only three experiments (p < 0.05. The total growth analysis identified four experiments as different from the control (p < 0.01. Three microcystin variants (MC-RR, MC-LR and MC-YR were quantified by liquid chromatography-tandem mass spectrometry. At the experimental end, the toxin content was unchanged when comparing cell growth in ASM-1 (N:P = 1, MLA and BG-11 (N:P = 10 medium. In all other experiments, the lowest microcystin production was observed from cells grown in Bold 3N medium during the exponential growth phase. The highest microcystin content was observed in cultures using BG-11(N:P = 100 medium.

  15. CONDITIONING MICROBIAL PRODUCTS CONTAINING NITROGEN FIXING BACTERIA WITH DIFFERENT SOLID EXCIPIENTS

    Directory of Open Access Journals (Sweden)

    VINTILĂ T.

    2007-01-01

    Full Text Available The stability in real time of two strains of Rhizobium (Rhizobium meliloti andRhizobium japonicum mixed with different excipients was evaluated during a6-months period. The excipients studied were: peat, peat and calciumcarbonate, zeolite, and ceramic. Liquid cultures and excipients mixtures weredried (12-14% humidity, sealed in plastic bags and preserved at +4oC. Thecells were activated periodically by suspending aliquots from dry products in0.9% saline solution. The viability of Rhizobium cells was evaluated bycultivation of diluted suspensions in YMA plates. The number of viable cells isdecreasing during drying in all cases, increase in the first month of storage,and remains constant or decrease very slowly during storage for all obtaineddry products containing rhizobia mixed with solid dry excipients. The highestnumber of viable cells at the end of the experiment was obtained in ceramicwith Rhizobium japonicum (8x105 cells/gram, and the lowest number ofviable cells was obtained in zeolite with Rhizobium meliloti (1,1x103cells/gram.

  16. CONDITIONING MICROBIAL PRODUCTS CONTAINING NITROGEN FIXING BACTERIA WITH DIFFERENT SOLID EXCIPIENTS

    Directory of Open Access Journals (Sweden)

    DANIELA VINTILĂ

    2007-05-01

    Full Text Available The stability in real time of two strains of Rhizobium (Rhizobium meliloti and Rhizobium japonicum mixed with different excipients was evaluated during a 6- months period. The excipients studied were: peat, peat and calcium carbonate, zeolite, and ceramic. Liquid cultures and excipients mixtures were dried (12-14% humidity, sealed in plastic bags and preserved at +4oC. The cells were activated periodically by suspending aliquots from dry products in 0.9% saline solution. The viability of Rhizobium cells was evaluated by cultivation of diluted suspensions in YMA plates. The number of viable cells is decreasing during drying in all cases, increase in the first month of storage, and remains constant or decrease very slowly during storage for all obtained dry products containing rhizobia mixed with solid dry excipients. The highest number of viable cells at the end of the experiment was obtained in ceramic with Rhizobium japonicum (8x105 cells/gram, and the lowest number of viable cells was obtained in zeolite with Rhizobium meliloti (1,1x103 cells/gram.

  17. Determination of radiography optimum conditions for complex shape products by simplex planning method

    International Nuclear Information System (INIS)

    Kipnis, M.A.; Korsunskij, G.M.; Mironenko, A.F.

    1984-01-01

    It has been suggested that the optimum regime of radiography should be determined using the method of successive simplex planning, which can be convenient in the cases, when the result of testing can not be presented quantitatively. Besides, in this case there is no necessity in duplication of experiments, as even gross errors are automatically corrected with further simplex motion. A plan and results of experimental determination of the optimum regime of product radiography using the X-ray RUP-120-5-1 apparatus are presented. In the experiments described voltage, current intensity and radiography duration are varied. The quality of X-ray images is evaluated according to conventional ten-point scale, taking into account the quality of each projection. It has been established that application of simplex planning to determine regimes of X-ray radiography of different types of products permits to obtain high-quality roentgenograms with simultaneous decrease in the consumption of photomaterials and considerable decrease in the time of laboratory tests

  18. Water Quality, Essential Condition Sustaining the Health, Production, Reproduction in Cattle. A Review

    Directory of Open Access Journals (Sweden)

    Cristina Iuliana El Mahdy

    2016-11-01

    Full Text Available The main component of the body: the water, alongside with many function which it has,represents a constituent in the diet of animal. There are many and various factors that influence the daily water requirements of animals: some dependent on animal: and others dependent on the environment. Water quality administered to livestock must meet the requirements for potability prerequisite to maintaining the health, externalization full productive potential and sustaining breeding. Knowing the importance of water quality consists in the negative action which can exert on the body to exceeding certain thresholds translated through: reducing water consumption simultaneously with the decrease milk production, decreased feed conversion rate and average daily gain, degradation of health status by reducing the local resistance, decrease overall body resistance, metabolic, digestive, skeletal disorders and impaired reproduction sphere translated through:decreasing fertility, abortions; elements interfering with the absorption of other essential water body, producing chronic or acute poisoning. The water composition plays essential role depending on which is supplemented or not as the case the quantity of the macro and trace minerals from feedingstuff  according to the synergism or antagonism action between  the minerals present.

  19. The influence of the irradiation regime upon mycotoxins production under experimental conditions

    International Nuclear Information System (INIS)

    Uralova, M.; Patzeltova, N.; Havlik, F.

    1987-01-01

    The inactivation is discussed of the toxinogenic strain Aspergillus flavus following the application of gamma radiation to wheat. The effects of the applied dose and of the absorbed dose of ionizing radiation upon the inhibition of mycelium growth and toxin production were defined. Aflatoxin B 1 was determined by extraction in chloroform and developed on Silufol R within the chloroform-acetone system. The applied doses of gamma radiation (3-30 kGy) showed that the absorbed dose did not inhibit aflatoxin production. By combining the action of gamma radiation with humidity of the wheat (humidity 13 to 15%; 25% irradiation 6 kGy), inactivation was reached. With the help of toxicologico-genetical tests (the Dominant Lethal Mutations Test, the Three Generations Test) the influence was traced of contaminated, irradiated substrates upon the health of experimental animals. It follows from the results obtained that in long-term feeding with contaminated wheat irradiated with gamma rays no positive mutagenic activity was recorded. It allows to presume that wheat of 25% humidity contaminated with a weakly toxigenic strain Aspergillus flavus irradiated with a dose of 6 kGy, and wheat of a humidity of 13 to 15%, contaminated with a strongly toxinogenic strain of Aspergillus flavus, irradiated with a dose of 6 kGy, were of no genetic risk for white rats. (author). 3 tabs., 9 refs

  20. Continuous biohydrogen production from fruit wastewater at low pH conditions.

    Science.gov (United States)

    Diamantis, Vasileios; Khan, Abid; Ntougias, Spyridon; Stamatelatou, Katerina; Kapagiannidis, Anastasios G; Aivasidis, Alexander

    2013-07-01

    Biohydrogen production from a simulated fruit wastewater (soluble COD = 3.17 ± 0.10 g L⁻¹) was carried out in a continuous stirred tank reactor (CSTR) of 2 L operational volume without biomass inoculation, heat pre-treatment or pH adjustment, resulting in a low operational pH (3.75 ± 0.09). The hydraulic retention time (HRT) varied from 15 to 5 h. A strong negative correlation (p CSTR was operated under the same HRT. The biogas hydrogen content was estimated as high as 55.8 ± 2.3 % and 55.4 ± 2.5 % at 25 and 30 °C, respectively. The main fermentation end products were acetic and butyric acids, followed by ethanol. Significant differences (p CSTR at 25 or 30 °C were identified for butyric acid at almost all HRTs examined. Simulation of the acidogenesis process in the CSTR (based on COD and carbon balances) indicated the possible metabolic compounds produced at 25 and 30 °C reactions and provided an adequate fit of the experimental data.

  1. Significant OH production under surface cleaning and air cleaning conditions: Impact on indoor air quality.

    Science.gov (United States)

    Carslaw, N; Fletcher, L; Heard, D; Ingham, T; Walker, H

    2017-11-01

    We report measurements of hydroxyl (OH) and hydroperoxy (HO 2 ) radicals made by laser-induced fluorescence spectroscopy in a computer classroom (i) in the absence of indoor activities (ii) during desk cleaning with a limonene-containing cleaner (iii) during operation of a commercially available "air cleaning" device. In the unmanipulated environment, the one-minute averaged OH concentration remained close to or below the limit of detection (6.5×10 5  molecule cm -3 ), whilst that of HO 2 was 1.3×10 7  molecule cm -3 . These concentrations increased to ~4×10 6 and 4×10 8  molecule cm -3 , respectively during desk cleaning. During operation of the air cleaning device, OH and HO 2 concentrations reached ~2×10 7 and ~6×10 8  molecule cm -3 respectively. The potential of these OH concentrations to initiate chemical processing is explored using a detailed chemical model for indoor air (the INDCM). The model can reproduce the measured OH and HO 2 concentrations to within 50% and often within a few % and demonstrates that the resulting secondary chemistry varies with the cleaning activity. Whilst terpene reaction products dominate the product composition following surface cleaning, those from aromatics and other VOCs are much more important during the use of the air cleaning device. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  2. Optimization of the nitrous vapors experimental conditions production by nitric acid electrochemical reduction

    International Nuclear Information System (INIS)

    Lemaire, M.

    1996-01-01

    Gaseous nitrogen oxides (NO and NO 2 ) involved as oxidizing agents in nuclear fuel reprocessing can be produced by electrochemical reduction of nitric acid. This is an interesting alternative to the existing process because no wastes are generated. voltammetric studies on a platinum electrode show that two reduction potential regions are observed in concentrated nitric acid solutions, between 0,05 V SHE and between 0,5 V SHE and 1 V SHE . The highest potential region reduction mechanism was studied by: classical micro-electrolysis methods, macro-electrolysis methods, infrared spectroscopy coupled to electrochemistry. It was determined that the origin of nitric acid reduction is the electrochemical reduction of nitrous acid in nitric oxide which chemically reduces nitric acid. This reaction produces nitrous acid back which indicate an auto-catalytic behaviour of nitric acid reduction mechanism. Nitrogen dioxide evolution during nitric reduction can also explained by an other chemical reaction. If the potential value of platinum electrode is above 0,8 V SHE , products of the indirect nitric acid reduction are nitrous acid, nitrogen oxide and nitrogen dioxide. Below this value nitric oxide can be reduced in nitrous oxide. Thus the potential value is the most important parameter for the nitrogen oxides production selectivity. However, owing to the auto-catalytic character of the reduction mechanism, potential value can be controlled during intentiostatic industrial electrolysis. (author)

  3. Grain boundary sweeping and dissolution effects on fission product behavior under severe fuel damage accident conditions

    International Nuclear Information System (INIS)

    Rest, J.

    1985-10-01

    The theoretical FASTGRASS-VFP model has been used in the interpretation of fission gas, iodine, tellurium, and cesium release from severe-fuel-damage (SFD) tests performed in the PBF reactor in Idaho. A theory of grain boundary sweeping of gas bubbles, gas bubble behavior during fuel liquefaction (destruction of grain boundaries due to formation of a U-rich melt phase), and during U-Zr eutectic melting has been included within the FASTGRASS-VFP formalism. The grain-boundary-sweeping theory considers the interaction between the moving grain boundary and two distinct size classes of bubbles, those on grain faces and on grain edges. The theory of the effects of fuel liquefaction and U-Zr eutectic melting on fission product behavior considers the migration and coalescence of fission gas bubbles in either molten uranium, or a zircaloy-uranium eutectic melt. The FASTGRASS-VFP predictions, measured release rates from the above tests, and previously published release rates are compared and differences between fission product behavior in trace-irradiated and in normally irradiated fuel are highlighted

  4. Surface studies of niobium chemically polished under conditions for superconducting radio frequency (SRF) cavity production

    Science.gov (United States)

    Tian, Hui; Reece, Charles E.; Kelley, Michael J.; Wang, Shancai; Plucinski, Lukasz; Smith, Kevin E.; Nowell, Matthew M.

    2006-11-01

    The performance of niobium superconducting radiofrequency (SRF) accelerator cavities is strongly impacted by the topmost several nanometers of the active (interior) surface, especially as influenced by the final surface conditioning treatments. We examined the effect of the most commonly employed treatment, buffered chemical polishing (BCP), on polycrystalline niobium sheet over a range of realistic solution flow rates using electron back scatter diffraction (EBSD), stylus profilometry, atomic force microscopy, laboratory XPS and synchrotron (variable photon energy) XPS, seeking to collect statistically significant datasets. We found that the predominant general surface orientation is (1 0 0), but others are also present and at the atomic-level details of surface plane orientation are more complex. The post-etch surface exhibits micron-scale roughness, whose extent does not change with treatment conditions. The outermost surface consists of a few-nm thick layer of niobium pentoxide, whose thickness increases with solution flow rate to a maximum of 1.3-1.4 times that resulting from static solution. The standard deviation of the roughness measurements is ±30% and that of the surface composition is ±5%.

  5. Surface studies of niobium chemically polished under conditions for superconducting radio frequency (SRF) cavity production

    Energy Technology Data Exchange (ETDEWEB)

    Tian Hui [Thomas Jefferson National Accelerator Facility and College of William and Mary (United States); Reece, Charles E. [Thomas Jefferson National Accelerator Facility and College of William and Mary (United States); Kelley, Michael J. [Thomas Jefferson National Accelerator Facility and College of William and Mary (United States)]. E-mail: mkelley@jlab.org; Wang Shancai [Department of Physics, Boston University (United States); Plucinski, Lukasz [Department of Physics, Boston University (United States); Smith, Kevin E. [Department of Physics, Boston University (United States); Nowell, Matthew M. [EDAX TSL (United States)

    2006-11-30

    The performance of niobium superconducting radiofrequency (SRF) accelerator cavities is strongly impacted by the topmost several nanometers of the active (interior) surface, especially as influenced by the final surface conditioning treatments. We examined the effect of the most commonly employed treatment, buffered chemical polishing (BCP), on polycrystalline niobium sheet over a range of realistic solution flow rates using electron back scatter diffraction (EBSD), stylus profilometry, atomic force microscopy, laboratory XPS and synchrotron (variable photon energy) XPS, seeking to collect statistically significant datasets. We found that the predominant general surface orientation is (1 0 0), but others are also present and at the atomic-level details of surface plane orientation are more complex. The post-etch surface exhibits micron-scale roughness, whose extent does not change with treatment conditions. The outermost surface consists of a few-nm thick layer of niobium pentoxide, whose thickness increases with solution flow rate to a maximum of 1.3-1.4 times that resulting from static solution. The standard deviation of the roughness measurements is {+-}30% and that of the surface composition is {+-}5%.

  6. Surface Studies of Niobium Chemically Polished Under Conditions for Superconducting Radio Frequency (SRF) Cavity Production

    Energy Technology Data Exchange (ETDEWEB)

    Tian,H.; Reece, C.; Kelley, M.; Wang, S.; Plucinski, L.; Smith, K.; Nowell, M.

    2006-01-01

    The performance of niobium superconducting radiofrequency (SRF) accelerator cavities is strongly impacted by the topmost several nanometers of the active (interior) surface, especially as influenced by the final surface conditioning treatments. We examined the effect of the most commonly employed treatment, buffered chemical polishing (BCP), on polycrystalline niobium sheet over a range of realistic solution flow rates using electron back scatter diffraction (EBSD), stylus profilometry, atomic force microscopy, laboratory XPS and synchrotron (variable photon energy) XPS, seeking to collect statistically significant datasets. We found that the predominant general surface orientation is (1 0 0), but others are also present and at the atomic-level details of surface plane orientation are more complex. The post-etch surface exhibits micron-scale roughness, whose extent does not change with treatment conditions. The outermost surface consists of a few-nm thick layer of niobium pentoxide, whose thickness increases with solution flow rate to a maximum of 1.3-1.4 times that resulting from static solution. The standard deviation of the roughness measurements is {+-}30% and that of the surface composition is {+-}5%.

  7. High Level Ethanol Production by Nitrogen and Osmoprotectant Supplementation under Very High Gravity Fermentation Conditions

    Directory of Open Access Journals (Sweden)

    Pachaya Chan-u-tit

    2013-02-01

    Full Text Available Optimization of nutrient supplements i.e., yeast extract (1, 3 and 5 g·L−1, dried spent yeast (DSY: 4, 12 and 20 g·L−1 and osmoprotectant (glycine: 1, 3 and 5 g·L−1 to improve the efficiency of ethanol production from a synthetic medium under very high gravity (VHG fermentation by Saccharomyces cerevisiae NP 01 was performed using a statistical method, an L9 (34 orthogonal array design. The synthetic medium contained 280 g·L−1 of sucrose as a sole carbon source. When the fermentation was carried out at 30 °C, the ethanol concentration (P, yield (Yp/s and productivity (Qp without supplementation were 95.3 g·L−1, 0.49 g·g−1 and 1.70 g·L−1·h−1, respectively. According to the orthogonal results, the order of influence on the P and Qp values were yeast extract > glycine > DSY, and the optimum nutrient concentrations were yeast extract, 3; DSY, 4 and glycine, 5 g·L−1, respectively. The verification experiment using these parameters found that the P, Yp/s and Qp values were 119.9 g·L−1, 0.49 g g−1 and 2.14 g·L−1·h−1, respectively. These values were not different from those of the synthetic medium supplemented with 9 g·L−1 of yeast extract, indicating that DSY could be used to replace some amount of yeast extract. When sweet sorghum juice cv. KKU40 containing 280 g·L−1 of total sugar supplemented with the three nutrients at the optimum concentrations was used as the ethanol production medium, the P value (120.0 g·L−1 was not changed, but the Qp value was increased to 2.50 g·L−1·h−1.

  8. Light intensity and production parameters of phytocenoses cultivated on soil-like substrate under controlled [correction of controled] environment conditions.

    Science.gov (United States)

    Tikhomirov, A A; Ushakova, S A; Gribovskaya, I A; Tirranen, L S; Manukovsky, N S; Zolotukhin, I G; Karnachuk, R A; Gros, J B; Lasseur, Ch

    2003-01-01

    To increase the degree of closure of biological life support systems of a new generation, we used vermicomposting to involve inedible phytomass in the intra-system mass exchange. The resulting product was a soil-like substrate, which was quite suitable for growing plants (Manukovsky et al. 1996, 1997). However, the soil like substrate can be regarded as a candidate for inclusion in a system only after a comprehensive examination of its physical, chemical, and other characteristics. An important criterion is the ability of the soil-like substrate to supply the necessary mineral elements to the photosynthesizing component under the chosen cultivation conditions. Thus, the purpose of this work was to study the feasibility of enhancing the production activity of wheat and radish crops by varying the intensity of photosynthetically active radiation, without decreasing the harvest index. The increase of light intensity from 920 to 1150 micromoles m-2 s-1 decreased the intensity of apparent photosynthesis of the wheat crops and slightly increased the apparent photosynthesis of the radish crops The maximum total and grain productivity (kg/m2) of the wheat crops was attained at the irradiance of 920 micromoles m-2 s-1. Light intensity of 1150 micromoles m-2 s-1 decreased the productivity of wheat plants and had no significant effect on the productivity of the radish crops (kg/m2) as compared to 920 micromoles m-2 s-1. The qualitative and quantitative composition of microflora of the watering solution and substrate was determined by the condition of plants, developmental phase and light intensity. By the end of wheat growth under 1150 micromoles m-2 s-1 the numbers of bacteria of the coliform family and phytopathogenic bacteria in the watering solution and substrate were an order of magnitude larger than under other illumination conditions. The obtained data suggest that the cultivation of plants in a life support system on soil-like substrate from composts has a number of

  9. Rice yield estimation based on weather conditions and on technological level of production systems in Brazil

    Directory of Open Access Journals (Sweden)

    José Eduardo Boffino de Almeida Monteiro

    2013-02-01

    Full Text Available The objective of this work was to evaluate an estimation system for rice yield in Brazil, based on simple agrometeorological models and on the technological level of production systems. This estimation system incorporates the conceptual basis proposed by Doorenbos & Kassam for potential and attainable yields with empirical adjusts for maximum yield and crop sensitivity to water deficit, considering five categories of rice yield. Rice yield was estimated from 2000/2001 to 2007/2008, and compared to IBGE yield data. Regression analyses between model estimates and data from IBGE surveys resulted in significant coefficients of determination, with less dispersion in the South than in the North and Northeast regions of the country. Index of model efficiency (E1' ranged from 0.01 in the lower yield classes to 0.45 in higher ones, and mean absolute error ranged from 58 to 250 kg ha‑1, respectively.

  10. Correlation between Strawberry (Fragaria ananassa Duch. Productivity and Photosynthesis-related Parameters under Various Growth Conditions

    Directory of Open Access Journals (Sweden)

    Hyo Gil Choi

    2016-10-01

    Full Text Available In the present study, we investigated changes in chlorophyll fluorescence, photosynthetic parameters and fruit yields, as well as fruit phytochemical accumulation of strawberry (Fragaria ananassa Duch. that had been cultivated in a greenhouse under different combinations of light intensity and temperature. In plants grown with low light (LL photosystem II chlorophyll fluorescence was found to increase as compared with those grown under high light (HL. When strawberry plants were grown with temperature higher than 5◦C in addition to LL, they showed decrease in non-photochemical quenching (NPQ, photochemical quenching (qP, as well as chlorophyll fluorescence decrease ratio (RFd when compared with other combinations of light and temperature. Moreover, fruit yield of strawberry was closely correlated with chlorophyll fluorescence-related parameters such as NPQ, qP, and RFd, but not with the maximum efficiency of PS II (Fv/Fm. Although plant groups grown under different combinations of light and temperature showed almost comparable levels of photosynthesis rates (Pr when irradiated with low-intensity light, they displayed clear differences when measured with higher irradiances. Plants grown under HL with temperature above 10◦C showed the highest Pr, in contrast to the plants grown under LL with temperature above 5◦C. When the stomatal conductance and the transpiration rate were measured, plants of each treatment showed clear differences even when analyzed with lower irradiances. We also found that fruit production during winter season was more strongly influenced by growth temperature than light intensity. We suggest that fruit productivity of strawberry is closely associated with chlorophyll fluorescence and photosynthesis-related parameters during cultivation under different regimes of temperature and light.

  11. Renewable energy production support schemes for residential-scale solar photovoltaic systems in Nordic conditions

    International Nuclear Information System (INIS)

    Hirvonen, Janne; Kayo, Genku; Cao, Sunliang; Hasan, Ala; Sirén, Kai

    2015-01-01

    The objective of this study was to examine the effect of production-based support schemes on the economic feasibility of residential-scale PV systems (1–10 kW) in Finland. This was done by calculating the payback time for various sizes of newly installed PV systems for a Finnish detached house with district heating. Three types of economic support schemes (guaranteed selling price, fixed premiums and self-consumption incentives) were tested in an hourly simulation. The load of the building was based on real-life measurements, while PV output was simulated with TRNSYS software. The energy results were post-processed with economic data in MATLAB to find the payback time. Hourly electricity prices from the Nordic energy market were used with PV system prices from Finnish companies. Unsubsidised residential PV systems in Finland had payback times of more than 40 years. The production-based support for PV generation needs to be two to three times the buying price of electricity, to make it possible to pay back the initial investment in 20 years. Low capacity systems with more than 50% self-consumption (under 3 kW) were favoured by self-consumption incentives, while high capacity systems with less than 40% self-consumption (over 5 kW) were favoured by the FIT-type support schemes. - Highlights: • Unsubsidised residential PV is uneconomical in Finland. • Support rate must be 2 times the electricity price for reasonable payback time. • Even using all electricity on-site is not profitable enough without support. • Assumed real interest rate had great influence on payback time. • Hourly electricity prices are much lower than average values from Finnish statistics

  12. Optimization of Growth Conditions for Purification and Production of L-Asparaginase by Spirulina maxima

    Science.gov (United States)

    El Baroty, Gamal S.

    2016-01-01

    L-asparaginase (L-AsnA) is widely distributed among microorganisms and has important applications in medicine and in food technology sectors. Therefore, the ability of the production, purification, and characterization of AsnA from Spirulina maxima (SM) were tested. SM cultures grown in Zarrouk medium containing different N2 (in NaNO3 form) concentrations (1.25, 2.50, and 5.0 g/L) for 18 days contained a significant various quantity of dry biomass yields and AsnA enzyme levels. MS L-AsnA activity was found to be directly proportional to the N2 concentration. The cultures of SM at large scales (300 L medium, 5 g/L N2) showed a high AsnA enzyme activity (898 IU), total protein (405 mg/g), specific enzyme activity (2.21 IU/mg protein), and enzyme yield (51.28 IU/L) compared with those in low N2 cultures. The partial purification of crude MS AsnA enzyme achieved by 80% ammonium sulfate AS precipitated and CM-Sephadex C-200 gel filtration led to increases in the purification of enzyme with 5.28 and 10.91 times as great as that in SM crude enzymes. Optimum pH and temperature of purified AsnA for the hydrolyzate were 8.5 and 37 ± 0.2°C, respectively. To the best of our knowledge, this is the first report on L-asparaginase production in S. maxima. PMID:27525017

  13. Biochar and microbial signaling: production conditions determine effects on microbial communication

    Science.gov (United States)

    Masiello, Caroline A.; Chen, Ye; Gao, Xiaodong; Liu, Shirley; Cheng, Hsiao-Ying; Bennett, Matthew R.; Rudgers, Jennifer A.; Wagner, Daniel S.; Zygourakis, Kyriacos; Silberg, Jonathan J.

    2013-01-01

    Charcoal has a long soil residence time, which has resulted in its production and use as a carbon sequestration technique (biochar). A range of biological effects can be triggered by soil biochar that can positively and negatively influence carbon storage, such as changing the decomposition rate of organic matter and altering plant biomass production. Sorption of cellular signals has been hypothesized to underlie some of these effects, but it remains unknown whether the binding of biochemical signals occurs, and if so, on time scales relevant to microbial growth and communication. We examined biochar sorption of N-3-oxo-dodecanoyl-L-homoserine lactone, an acyl-homoserine lactone (AHL) intercellular signaling molecule used by many gram-negative soil microbes to regulate gene expression. We show that wood biochars disrupt communication within a growing multicellular system that is made up of sender cells that synthesize AHL and receiver cells that express green fluorescent protein in response to an AHL signal. However, biochar inhibition of AHL-mediated cell-cell communication varied, with the biochar prepared at 700°C (surface area of 301 m2/g) inhibiting cellular communication 10-fold more than an equivalent mass of biochar prepared at 300°C (surface area of 3 m2/g). These findings provide the first direct evidence that biochars elicit a range of effects on gene expression dependent on intercellular signaling, implicating the method of biochar preparation as a parameter that could be tuned to regulate microbial-dependent soil processes, like nitrogen fixation and pest attack of root crops. PMID:24066613

  14. Optimization of Growth Conditions for Purification and Production of L-Asparaginase by Spirulina maxima

    Directory of Open Access Journals (Sweden)

    Hanaa H. Abd El Baky

    2016-01-01

    Full Text Available L-asparaginase (L-AsnA is widely distributed among microorganisms and has important applications in medicine and in food technology sectors. Therefore, the ability of the production, purification, and characterization of AsnA from Spirulina maxima (SM were tested. SM cultures grown in Zarrouk medium containing different N2 (in NaNO3 form concentrations (1.25, 2.50, and 5.0 g/L for 18 days contained a significant various quantity of dry biomass yields and AsnA enzyme levels. MS L-AsnA activity was found to be directly proportional to the N2 concentration. The cultures of SM at large scales (300 L medium, 5 g/L N2 showed a high AsnA enzyme activity (898 IU, total protein (405 mg/g, specific enzyme activity (2.21 IU/mg protein, and enzyme yield (51.28 IU/L compared with those in low N2 cultures. The partial purification of crude MS AsnA enzyme achieved by 80% ammonium sulfate AS precipitated and CM-Sephadex C-200 gel filtration led to increases in the purification of enzyme with 5.28 and 10.91 times as great as that in SM crude enzymes. Optimum pH and temperature of purified AsnA for the hydrolyzate were 8.5 and 37 ± 0.2°C, respectively. To the best of our knowledge, this is the first report on L-asparaginase production in S. maxima.

  15. Biochar and microbial signaling: production conditions determine effects on microbial communication.

    Science.gov (United States)

    Masiello, Caroline A; Chen, Ye; Gao, Xiaodong; Liu, Shirley; Cheng, Hsiao-Ying; Bennett, Matthew R; Rudgers, Jennifer A; Wagner, Daniel S; Zygourakis, Kyriacos; Silberg, Jonathan J

    2013-10-15

    Charcoal has a long soil residence time, which has resulted in its production and use as a carbon sequestration technique (biochar). A range of biological effects can be triggered by soil biochar that can positively and negatively influence carbon storage, such as changing the decomposition rate of organic matter and altering plant biomass production. Sorption of cellular signals has been hypothesized to underlie some of these effects, but it remains unknown whether the binding of biochemical signals occurs, and if so, on time scales relevant to microbial growth and communication. We examined biochar sorption of N-3-oxo-dodecanoyl-L-homoserine lactone, an acyl-homoserine lactone (AHL) intercellular signaling molecule used by many gram-negative soil microbes to regulate gene expression. We show that wood biochars disrupt communication within a growing multicellular system that is made up of sender cells that synthesize AHL and receiver cells that express green fluorescent protein in response to an AHL signal. However, biochar inhibition of AHL-mediated cell-cell communication varied, with the biochar prepared at 700 °C (surface area of 301 m(2)/g) inhibiting cellular communication 10-fold more than an equivalent mass of biochar prepared at 300 °C (surface area of 3 m(2)/g). These findings provide the first direct evidence that biochars elicit a range of effects on gene expression dependent on intercellular signaling, implicating the method of biochar preparation as a parameter that could be tuned to regulate microbial-dependent soil processes, like nitrogen fixation and pest attack of root crops.

  16. Productivity and seed health of husked oats (Avena sativa L. grown under different soil moisture conditions

    Directory of Open Access Journals (Sweden)

    Agnieszka Pszczółkowska

    2012-12-01

    Full Text Available The present study investigated the effect of different soil moisture content levels (60 - 70% SWC (soil water capacity - control; 30 - 35% SWC - water stress on yields, gas exchange parameters, seed health, and protein fractions of husked oat grain. The study showed that water deficit resulted in a decrease in grain weight per plant and a reduction in the gas exchange rates, primarily the photosynthesis and transpiration rates. Cladosporium cladosporioides was the dominant species on oat kernels in both experimental treatment options and in both years of the study. The presence of Fusarium poae was also found. Higher contents of prolamin, albumin and globulin fractions were found in the oat grain harvested from plants grown under soil water deficit conditions.

  17. Sustaining soil productivity by integrated plant nutrient management in wheat based cropping system under rainfed conditions

    International Nuclear Information System (INIS)

    Dilshad, M.; Lone, M.I.

    2011-01-01

    The study of the use of organic (FYM) and inorganic (NPK) nutrient sources with bio fertiliser on wheat-fallow and wheat-maize cropping system under rainfed environment revealed significant increase in bio metric parameters of wheat during winter and summer seasons of two years. During both the seasons, application of half NPK + half FYM + Bio power (brand) produced the highest grain yield (3684 kg/ha) and (3781 kg/ha) of wheat with the maximum N uptake of 357 kg/ha, P uptake of 51 kg/ha and K uptake of 215 kg/ha. Wheat-maize cropping system was found to be profitable economically with integrated use of mineral and organic and/or Bio power under rainfed conditions of Pakistan. (author)

  18. submitter Elemental composition and clustering behaviour of α-pinene oxidation products for different oxidation conditions

    CERN Document Server

    Praplan, A P; Bianchi, F; Rissanen, M P; Ehn, M; Jokinen, T; Junninen, H; Adamov, A; Amorim, A; Dommen, J; Duplissy, J; Hakala, J; Hansel, A; Heinritzi, M; Kangasluoma, J; Kirkby, J; Krapf, M; Kürten, A; Lehtipalo, K; Riccobono, F; Rondo, L; Sarnela, N; Simon, M; Tomé, A; Tröstl, J; Winkler, P M; Williamson, C; Ye, P; Curtius, J; Baltensperger, U; Donahue, N M; Kulmala, M; Worsnop, D R

    2015-01-01

    This study presents the difference between oxidised organic compounds formed by α-pinene oxidation under various conditions in the CLOUD environmental chamber: (1) pure ozonolysis (in the presence of hydrogen as hydroxyl radical (OH) scavenger) and (2) OH oxidation (initiated by nitrous acid (HONO) photolysis by ultraviolet light) in the absence of ozone. We discuss results from three Atmospheric Pressure interface Time-of-Flight (APi-TOF) mass spectrometers measuring simultaneously the composition of naturally charged as well as neutral species (via chemical ionisation with nitrate). Natural chemical ionisation takes place in the CLOUD chamber and organic oxidised compounds form clusters with nitrate, bisulfate, bisulfate/sulfuric acid clusters, ammonium, and dimethylaminium, or get protonated. The results from this study show that this process is selective for various oxidised organic compounds with low molar mass and ions, so that in order to obtain a comprehensive picture of the elemental composition o...

  19. Performance of maize cultivars for fodder production under rainfed conditions of pothohar tract

    International Nuclear Information System (INIS)

    Gurmani, Z.A.; Zahid, M.S.

    2008-01-01

    For finding the forage yield potential of eight maize cultivars, the cultivars were sown in the month of July during both consecutive years 2001 and 2002 and harvested in the month of September in both the years. The cultivars differed significantly from one another with regard to plant height, number of leaves per tiller, number of plants per row, leaf area per plant, green fodder and dry matter yield. The cultivar, Akbar produced taller plants, with the largest leaf area among and more number of leave per tiller, and consequently yielded highest amount of green as well as dry matter among all the varieties under the rainfed climatic conditions of Pothohar tract of Pakistan. (author)

  20. Evaluation of water quality conditions near proposed fish production sites associated with the Yakima Fisheries Project. Final report

    International Nuclear Information System (INIS)

    Dauble, D.d.; Mueller, R.P.; Martinson, G.A.

    1994-05-01

    In 1991, the Pacific Northwest Laboratory (PNL) began studying water quality at several sites in the Yakima River Basin for the Bonneville Power Administration. These sites were being proposed as locations for fish culture facilities as part of the Yakima Fisheries Project (YFP). Surface water quality parameters near the proposed fish culture facilities are currently suitable for fish production. Water quality conditions in the mainstream Yakima River and its tributaries are generally excellent in the upper part of the watershed (i.e., near Cle Elum), but they are only fair to poor for the river downstream of Union Gap (river mile 107). Water quality of the Naches River near Oak Flats is also suitable for fish production. Groundwater supplies near the proposed fish production facilities typically have elevated concentrations of metals and dissolved gases. These conditions can be mitigated using best engineering practices such as precipitation and degasification. Additionally, mixing with surface water may improve these conditions. Depending on the location and depth of the well, groundwater temperatures may be warmer than optimum for acclimating and holding juvenile and adult fish. Water quality parameters measured in the Yakima River and tributaries sometimes exceed the range of values described as acceptable for culture of salmonids and for the protection of other aquatic life. However, constituent concentrations are within ranges that exist in many northwest fish hatcheries. Additionally, site-specific tests conducted by PNL (i.e., live box exposures and egg incubation studies) indicate that fish can be successfully reared in surface and well water near the proposed facility sites. Thus, there appear to be no constraints to artificial production for the YFP

  1. Increased ophthalmic acid production is supported by amino acid catabolism under fasting conditions in mice.

    Science.gov (United States)

    Kobayashi, Sho; Lee, Jaeyong; Takao, Toshifumi; Fujii, Junichi

    2017-09-23

    Glutathione (GSH) plays pivotal roles in antioxidation and detoxification. The transsulfuration pathway, in conjunction with methionine metabolism, produces equimolar amounts of cysteine (Cys) and 2-oxobutyric acid (2OB). The resulting 2OB is then converted into 2-aminobutyric acid (2AB) by a transaminase and is utilized as a substitute for Cys by the GSH-synthesizing machinery to produce ophthalmic acid (OPT). By establishing a method for simultaneously measuring Cys, GSH, and OPT by liquid chromatography-mass spectrometry, we found that fasting causes an elevation in OPT levels in the liver and blood plasma, even though the levels of Cys and GSH are decreased. Autophagy was activated, but the levels of GSH/OPT-synthesizing enzymes remained unchanged. After 6 h of fasting, the mice were given 1% 2AB and/or 5% glucose in the drinking water for an additional 24 h and the above metabolites analyzed. 2AB administration caused an increase in OPT levels, and, when glucose was co-administered with 2AB, the levels of OPT were elevated further but GSH levels were decreased somewhat. These results suggest that, while Cys is utilized for glyconeogenesis under fasting conditions, reaching levels that were insufficient for the synthesis of GSH, 2OB was preferentially converted to 2AB via amino acid catabolism and was utilized as a building block for OPT. Thus the consumption of Cys and the parallel elevation of 2AB under fasting conditions appeared to force γ-glutamylcysteine synthetase to form γ-glutamyl-2AB, despite the fact that the enzyme has a higher Km value for 2AB than Cys. Copyright © 2017 Elsevier Inc. All rights reserved.

  2. Effect of growth conditions on microbial activity and iron-sulfide production by Desulfovibrio vulgaris

    International Nuclear Information System (INIS)

    Zhou, Chen; Vannela, Raveender; Hayes, Kim F.; Rittmann, Bruce E.

    2014-01-01

    Highlights: • Extended incubation time to 16 days allowed significant FeS crystallization. • A weakly acidic pH greatly enhanced particle growth of mackinawite. • Microbial metabolism of different donors systematically altered the ambient pH. • Greater sulfide accumulation stimulated mackinawite transformation to greigite. - Abstract: Sulfate-reducing bacteria (SRB) can produce iron sulfide (FeS) solids with mineralogical characteristics that may be beneficial for a variety of biogeochemical applications, such as long-term immobilization of uranium. In this study, the growth and metabolism of Desulfovibrio vulgaris, one of the best-studied SRB species, were comprehensively monitored in batch studies, and the biogenic FeS solids were characterized by X-ray diffraction. Controlling the pH by varying the initial pH, the iron-to-sulfate ratio, or the electron donor – affected the growth of D. vulgaris and strongly influenced the formation and growth of FeS solids. In particular, lower pH (from initial conditions or a decrease caused by less sulfate reduction, FeS precipitation, or using pyruvate as the electron donor) produced larger-sized mackinawite (Fe 1+x S). Greater accumulation of free sulfide, from more sulfate reduction by D. vulgaris, also led to larger-sized mackinawite and particularly stimulated mackinawite transformation to greigite (Fe 3 S 4 ) when the free sulfide concentration was 29.3 mM. Furthermore, sufficient free Fe 2+ led to the additional formation of vivianite [Fe 3 (PO 4 ) 2 ·8(H 2 O)]. Thus, microbially relevant conditions (initial pH, choice of electron donor, and excess or deficiency of sulfide) are tools to generate biogenic FeS solids of different characteristics

  3. Essais d'adaptation de production massive de juvéniles de Clarias gariepinus en conditions rurales

    Directory of Open Access Journals (Sweden)

    Micha, JC.

    2005-01-01

    Full Text Available Adaptive Trials for Intensive Clarias gariepinus fingerling to Rural Conditions. Artificial reproduction and fingerling production of the African catfish Clarias gariepinus (Burchell 1822 have been tested under less controlled conditions to adapt the intensive technology used by Aquafarm (Tihange, Belgium to rural conditions present in developing countries. The main aim was to compare the efficiency of ovaprim a synthesised hormone with catfish pituitary extracts. Fecundity and quality of eggs (egg weight were not significantly different. Incubation results on water hyacinth and on mosquito mesh frames were compared to those obtained in Zoug jars; the latter showed a higher hatching rate (67% than water hyacinth (39% and mosquito mesh frames (44%. Larvae were stocked at different stocking rates in ponds (T1: 800 larvae.m-3 and T2: 1600 larvae. m-3 and in tanks (T3: 3200 larvae.m-3 et T4: 6400 larvae.m-3. After a 50-day nursing period, no significant difference (P> 0.05 was observed for specific growth rate (SGR and survival rate, but better SGR were observed in ponds (18.34%.d-1 than in tanks (16.81%.d-1. In general, production was slightly higher in tanks than in ponds being related to a higher stocking density. Significantly higher harvests were obtained from tanks with higher stocking densities but the sizes of the larvae were very heterogeneous. In such case, it is recommended to grade the larvae at 6 or 7 days intervals using different mesh sizes (from 3 to 5 mm. These less intensive rearing conditions gave nevertheless good fingerling productions.

  4. Biohydrogen and methane production by co-digestion of cassava stillage and excess sludge under thermophilic condition.

    Science.gov (United States)

    Wang, Wen; Xie, Li; Chen, Jinrong; Luo, Gang; Zhou, Qi

    2011-02-01

    Thermophilic anaerobic hydrogen and methane production by co-digestion of cassava stillage (CS) and excess sludge (ES) was investigated in this study. The improved hydrogen and subsequent methane production were observed by co-digestion of CS with certain amount of ES in batch experiments. Compared with one phase anaerobic digestion, two phase anaerobic digestion offered an attractive alternative with more abundant biogas production and energy yield, e.g., the total energy yield in two phase obtained at VS(CS)/VS(ES) of 3:1 was 25% higher than the value of one phase. Results from continuous experiments further demonstrated that VS(CS)/VS(ES) of 3:1 was optimal for hydrogen production with the highest hydrogen yield of 74 mL/gtotal VS added, the balanced nutrient condition with C/N ratio of 1.5 g carbohydrate-COD/gprotein-COD or 11.9 g C/gN might be the main reason for such enhancement. VS(CS)/VS(ES) of 3:1 was also optimal for continuous methane production considering the higher methane yield of 350 mL/gtotal VS added and the lower propionate concentration in the effluent. Copyright © 2010 Elsevier Ltd. All rights reserved.

  5. Optimization of culture conditions and medium composition for the production of micrococcin GO5 by Micrococcus sp. GO5.

    Science.gov (United States)

    Kim, Mi-Hee; Kong, Yoon-Jung; Baek, Hong; Hyun, Hyung-Hwan

    2006-01-02

    To enhance the production of micrococcin GO5, a bacteriocin produced by Micrococcus sp. GO5, cultivation conditions and medium composition were optimized. The optimal initial pH and temperature for bacteriocin production were 7.0-9.0 and 37 degrees C, respectively. Micrococcus sp. GO5 displayed the highest micrococcin GO5 activity when grown in modified MRS medium that contained lactose or sucrose, rather than glucose, as a carbon source. The maximum bacteriocin activity was obtained in modified MRS medium containing 0.5% tryptone and 1.0% yeast extract as nitrogen sources instead of the other nitrogen sources present in MRS medium. Bacteriocin production was greatly affected by the concentration of K(2)HPO(4); strain GO5 produced eight-fold more bacteriocin in medium containing 2.0-2.5% K(2)HPO(4) than in medium containing 0.2% K(2)HPO(4). The optimal concentration of MgSO(4).7H(2)O for bacteriocin production was 0.5%. The production of micrococcin GO5 was increased 32-fold in shake flask culture and 16-fold in a bioreactor using the optimized medium (TY medium), compared with culturing in MRS medium.

  6. Effect of the Characters of Chitosans Used and Regeneration Conditions on the Yield and Physicochemical Characteristics of Regenerated Products

    Directory of Open Access Journals (Sweden)

    Chu Hsi Hsu

    2015-04-01

    Full Text Available The objective of this study was to explore the effect of the character of chitosans used, and the regeneration conditions employed on, the yield and physicochemical characteristics of regenerated products. Different concentrations of acetic acid were used to dissolve chitosans of 61.7% and 94.9% degree of deacetylation (DD, and weight-average molecular weight (Mw of 176 and 97 kDa, respectively; they were then precipitated with an 8 N NaOH solution, followed by washing and neutral and freeze drying to get the regenerated products. Yields of regenerated products and their physicochemical properties, such as ash content, bulk density, Mw, polydispersity index (PDI, DD, and crystallinity were measured. A higher concentration of acetic acid used resulted in a higher yield. The purity of the regenerated product increased significantly, whereas the bulk density and crystallinity decreased significantly after regeneration. The regeneration process showed its merits of narrowing down the PDI of regenerated products. The DD and structure of chitosan was changed insignificantly after the regeneration process.

  7. TENORM Characterisation, Conditioning and Remediation to be Reused in New Products and Associated Possible Public Member and Occupational Hazard

    International Nuclear Information System (INIS)

    Prlic, I.; Marovic, G.; Turk, R.

    2010-01-01

    If the TENORM is a byproduct originating from any energy gaining technological production process is to be reused as a product-raw material for other advanced product it has to meet the ultimate condition regulating the content of NORM or TENORM material in a final product. It means that the minimum requirements to be achieved are to mix the TENORM material with any other suitable material resulting in final H ex less than unity (H ex smaller than 1) for any quantity of TENORM mixed. Despite of a present lack of national legislation covering the use and handling of NORM and TENORM we can use the existing worldwide recommendations based on thorough radiological characterization. If the recommended UNSCEAR 2000 limit values are used as a base for discussion demanding that the TENORM is to be mixed with other suitable materials and monitored before further reuse as a constructing material product, especially constructing material for human housing, the listed quantities: Ra eq , H ex , D(nGy/h), H in , I yr , D ind (mSv/yr), D out (mSv/yr), Sampling site-GPS (coordinates of TENORM samples) calculated from the measured dose rate and activity concentration data will be the key quantities describing the radiological characterization of any TENORM facility. The reuse of TENORM in a new product is recommended (if economically cost-effective) but only under the professional supervision of total radionuclide activity content in final product. We are presenting the public hazard assessment procedure at phosphogypsum tailing facility as a specific TENORM site which has nothing to do with dangerous or hazardous radwaste in the sense of the existing legal legislation, not EU nor national Croatian. The EU and national legislation based on the definition of reference levels of radio nuclides present in TENORM is in process and Article 35 of Euratom Treaty is in process to be taken into account in future.(author).

  8. Mineral-assisted production of benzene under hydrothermal conditions: Insights from experimental studies on C6 cyclic hydrocarbons

    Science.gov (United States)

    Venturi, Stefania; Tassi, Franco; Gould, Ian R.; Shock, Everett L.; Hartnett, Hilairy E.; Lorance, Edward D.; Bockisch, Christiana; Fecteau, Kristopher M.; Capecchiacci, Francesco; Vaselli, Orlando

    2017-10-01

    Volatile Organic Compounds (VOCs) are ubiquitously present at low but detectable concentrations in hydrothermal fluids from volcanic and geothermal systems. Although their behavior is strictly controlled by physical and chemical parameters, the mechanisms responsible for the production of most VOCs in natural environments are poorly understood. Among them, benzene, whose abundances were found to be relatively high in hydrothermal gases, can theoretically be originated from reversible catalytic reforming processes, i.e. multi-step dehydrogenation reactions, involving saturated hydrocarbons. However, this hypothesis and other hypotheses are difficult to definitively prove on the basis of compositional data obtained by natural gas discharges only. In this study, therefore, laboratory experiments were carried out to investigate the production of benzene from cyclic hydrocarbons at hydrothermal conditions, specifically 300 °C and 85 bar. The results of experiments carried out in the presence of water and selected powdered minerals, suggest that cyclohexane undergoes dehydrogenation to form benzene, with cyclohexene and cyclohexadiene as by-products, and also as likely reaction intermediates. This reaction is slow when carried out in water alone and competes with isomerization and hydration pathways. However, benzene formation was increased compared to these competing reactions in the presence of sulfide (sphalerite and pyrite) and iron oxide (magnetite and hematite) minerals, whereas no enhancement of any reaction products was observed in the presence of quartz. The production of thiols was observed in experiments involving sphalerite and pyrite, suggesting that sulfide minerals may act both to enhance reactivity and also as reactants after dissolution. These experiments demonstrate that benzene can be effectively produced at hydrothermal conditions through dehydrogenation of saturated cyclic organic structures and highlight the crucial role played by minerals in this

  9. Exploring the possibility of using Agroplus Biodecomposer for boosting up rice productivity under Bangladesh condition

    Directory of Open Access Journals (Sweden)

    Dulaly Sarker

    2018-04-01

    Full Text Available Over dependence on chemical fertilizers is a threat to the sustainability of rice ecosystem. Application of organic and biofertilizers might reduce reliance on chemical fertilizers and thus can play a vital role to boost up rice productivity in an eco-friendly way. An experiment was conducted at Mymensingh (24°10'0'' N latitude and 90°25'0" E longitude at 15 m above the sea level, Bangladesh during November 2015 to April 2016 to evaluate the effect of different dosages of Agroplus Biodecomposer, an organic biofertilizer containing Streptomycetes bacteria, on the growth and yield performance of some rice. The experiment included four winter rice varieties viz. (i Hybrid rice Hira and (ii Hybrid rice Tej (iii BRRI dhan28 and (iv BRRI dhan29; and four concentrations of Agroplus Biodecomposer viz. (i no Agroplus Biodecomposer (Control, (ii 2% Agroplus Biodecomposer (iii 3% Agroplus Biodecomposer and (iv 4% Agroplus Biodecomposer. The experiment was laid out in a randomized complete block design (RCBD with three replications. Agroplus Biodecomposer positively influenced growth and productivity of winter rice. It was evident that both plant height and tillering ability of winter rice were increased gradually with increased concentration of Agroplus Biodecomposer at all the growth stages of rice. All the yield contributing characters of rice were enhanced due to Agroplus Biodecomposer application which resulted in increased grain yield. Compared to control, rice grain yield was increased by 14, 20 and 28%, respectively due to application of Agroplus Biodecomposer at 2, 3 and 4% concentration. Rice variety also differed significantly in terms of growth and yield performance among themselves. Hybrid varieties performed better than inbred ones. Hybrid variety Hira appeared as the best performer followed by another hybrid Tej. Hybrid variety Hira interacted favorably with 4% Agroplus Biodecomposer to produce the highest grain yield of rice (7 t ha-1

  10. Absorption of gamma-emitting fission products and activation products by rice under flooded and unflooded conditions from two tropical soils

    International Nuclear Information System (INIS)

    D'Souza, T.J.; Mistry, K.B.

    1980-01-01

    The absorption of gamma-emitting fission products 106 Ru, 125 Sb, 137 Cs and 144 Ce and activation products 59 Fe, 58 Co, 54 Mn and 65 Zn by rice plants grown on two contrasting tropical soils, namely, a blak soil (pellustert) and a laterite (oxisol), and the effects of flooding were studied under controlled conditions. Results indicated greater uptake of 106 Ru and 125 Sb from the black soil than from the laterite. In contrast, the uptake of 144 Ce and 137 Cs was greater in the laterite than in the black soil. Flooding treatment enhanced the uptake of all these fission products by rice plants in the laterite soil whereas this effect was observed only for 125 Sb and 137 Cs in the black soil. The plant uptake of activation products from the two soil types showed maximum accumulation of 65 Zn followed by 54 Mn, 59 Fe and 58 Co in both soil types. Besides, uptake of these nuclides was greater from the laterite soil than from the black soil. Flooding treatment for rice while showing a reduction of 59 Fe uptake showed an increase in plant uptake of 58 Co, 54 Mn and 65 Zn in both soil types. (orig.)

  11. Absorption of gamma-emitting fission products and activation products by rice under flooded and unflooded conditions from two tropical soils

    Energy Technology Data Exchange (ETDEWEB)

    D' Souza, T J; Mistry, K B [Bhabha Atomic Research Centre, Bombay (India). Biology and Agriculture Div.

    1980-01-01

    The absorption of gamma-emitting fission products /sup 106/Ru, /sup 125/Sb, /sup 137/Cs and /sup 144/Ce and activation products /sup 59/Fe, /sup 58/Co, /sup 54/Mn and /sup 65/Zn by rice plants grown on two contrasting tropical soils, namely, a blak soil (pellustert) and a laterite (oxisol), and the effects of flooding were studied under controlled conditions. Results indicated greater uptake of /sup 106/Ru and /sup 125/Sb from the black soil than from the laterite. In contrast, the uptake of /sup 144/Ce and /sup 137/Cs was greater in the laterite than in the black soil. Flooding treatment enhanced the uptake of all these fission products by rice plants in the laterite soil whereas this effect was observed only for /sup 125/Sb and /sup 137/Cs in the black soil. The plant uptake of activation products from the two soil types showed maximum accumulation of /sup 65/Zn followed by /sup 54/Mn, /sup 59/Fe and /sup 58/Co in both soil types. Besides, uptake of these nuclides was greater from the laterite soil than from the black soil. Flooding treatment for rice while showing a reduction of /sup 59/Fe uptake showed an increase in plant uptake of /sup 58/Co, /sup 54/Mn and /sup 65/Zn in both soil types.

  12. Rapid and slow pyrolysis of pistachio shell: effect of pyrolysis conditions on the product yields and characterization of the liquid product

    Energy Technology Data Exchange (ETDEWEB)

    Putun, Ayse E [Department of Chemical Engineering, Anadolu University, Eskisehir 26470, (Turkey); Ozbay, Nurgul [Bozuyuk Vocational School, Anadolu University, Bozuyuk/Bilecik, (Turkey); Varol, Esin Apaydin; Uzun, Basak B; Ates, Fuda [Department of Chemical Engineering, Anadolu University, Eskisehir 26470, (Turkey)

    2006-10-30

    This study reports the experimental results for the pyrolysis of pistachio shell under different conditions in a tubular reactor under a nitrogen flow. For the different conditions of pyrolysis temperature, nitrogen flow rate and heating rate, pyrolysis temperature of 773 K gave the highest bio-oil yield with a value of 27.7% when the heating rate and carrier gas flow rate were chosen as 300 K min{sup -1} and 100 cm{sup 3} min{sup -1}, respectively. Column chromatography was applied to this bio-oil and its subfractions were characterized by elemental analysis, FT-IR and 1H-NMR. Aliphatic subfraction was conducted to gas chromatography-mass spectroscopy for further characterization. The results for the characterization show that using pistachio shell as a renewable source to produce valuable liquid products is applicable via pyrolysis. (Author)

  13. Surface reactivity of colloidal corrosion product and alloys in PWR conditions

    International Nuclear Information System (INIS)

    Lefevre, Gregory; Leclercq, Stephanie; Cabanas, Bruna-Martin; Delaunay, Sophie; Mansour, Carine; Berger, Gilles

    2012-09-01

    The corrosion of metallic components of water circuits of Pressurized Water Reactors generates colloidal particles. These particles are transported in the circuits, they sorb dissolved species and they can deposit on alloys in given parts of the circuits. Sorption and deposition generate several technical drawbacks in both primary and secondary circuits. According to the DLVO theory, adhesion between two surfaces is controlled by electrostatic and Van der Waals forces. The latter are always attractive and does not depends on solution chemistry. On the contrary, electrostatic forces are connected to the surface charge and depend strongly on the chemical properties of the solids and on the chemistry of the solution. Depending on the relative charge of the surfaces, these forces are attractive or repulsive and can have a major effect on the deposition behavior of particles. According to the surface complexation theory, the surface charge of metallic oxides results from sorption or desorption of protons, leading to positive or negative surface sites, and thus, strongly depends on the solution pH. Dissolved species can sorb on the surface, depending on the ionic charge of these species and on the surface charge. Thus, the knowledge of the surface charge of corrosion particles and alloys, their affinity towards several ions as protons, nickel, cobalt, sulfate, or borate ions has been shown to be useful to predict the transport of the contamination in the primary circuit, or to understand the accumulation of impurities in the steam generator in the secondary circuit. At room temperature, these data can be easily measured, or found in literature. In PWR conditions (high temperature, high pressure), most of the usual protocols and commercial instruments cannot be used. For several years, collaboration between EDF R and D and CNRS has been developed to get information about the surface reactivity of iron oxides, ferrites, and alloys in such conditions. Some of the results

  14. THE SEED PRODUCTIVITY OF PLANTS IN CENOPOPULATION TULIPA BIFLORA UNDER THE CONDITIONS OF The Republic of Kalmykia

    Directory of Open Access Journals (Sweden)

    Aleksandra Sergeevna Ochirova

    2017-12-01

    Full Text Available Background: Viable seeds in the composition of populations are considered as an indicator of the life strategy of species. Hence, seed productivity can be considered as an important indicator of species adaptation to habitat conditions. The replaceable productivity of bulbous ephemeroids growing in arid conditions has not been studied sufficiently. In connection with the foregoing, our goal was to identify the seed productivity of plants in the tulip cenopopulations of the two-flowered tulip – Tulipa biflora Pall. (Liliaceae, which is recommended for protection in the conditions of Kalmykia. Materials and metods: Three cenopopulations of Tulipa biflora were studied in different plant communities on brown light loamy solonetzic soils. The sample size was no less than 30 plants of Tulipa biflora, their mature fruits were considered in the aspects of the measured features, their potential and actual seed production, as well as the percentage of seminification. The density of generative plants was taken into account at 10 sites of 0.5 square meters, located along the transect. Resalts: Biometric signs of the fetus in plants in Tulipa biflora populations were positively correlated with air temperature during the active vegetation period of the species. The potential and actual seed productivity of plants in the Tulipa biflora cenpopulations in 2017 is more than in 2016, however, the percentage of seminification marked the reverse results: in 2017 it was 4.7–10.4% lower. In this case, the plants of one of the Tulipa biflora cenopopulations, showed the greatest percentage of seminification in both studies: in 2016 – 77.5, in 2017 – 67.1 pieces of seeds per plant. The mature seed bank which is formed during the growing season in the cenopopulations of Tulipa biflora during the years under the study amounted to 782.8–1338.4 seeds per 1.0 sq.m. Conclusion: The idea of a bank of mature seeds was got – 782.8–1338.4 seeds per 1.0 m2, which is

  15. Online Condition Monitoring of Bearings to Support Total Productive Maintenance in the Packaging Materials Industry.

    Science.gov (United States)

    Gligorijevic, Jovan; Gajic, Dragoljub; Brkovic, Aleksandar; Savic-Gajic, Ivana; Georgieva, Olga; Di Gennaro, Stefano

    2016-03-01

    The packaging materials industry has already recognized the importance of Total Productive Maintenance as a system of proactive techniques for improving equipment reliability. Bearing faults, which often occur gradually, represent one of the foremost causes of failures in the industry. Therefore, detection of their faults in an early stage is quite important to assure reliable and efficient operation. We present a new automated technique for early fault detection and diagnosis in rolling-element bearings based on vibration signal analysis. Following the wavelet decomposition of vibration signals into a few sub-bands of interest, the standard deviation of obtained wavelet coefficients is extracted as a representative feature. Then, the feature space dimension is optimally reduced to two using scatter matrices. In the reduced two-dimensional feature space the fault detection and diagnosis is carried out by quadratic classifiers. Accuracy of the technique has been tested on four classes of the recorded vibrations signals, i.e., normal, with the fault of inner race, outer race, and ball operation. The overall accuracy of 98.9% has been achieved. The new technique can be used to support maintenance decision-making processes and, thus, to increase reliability and efficiency in the industry by preventing unexpected faulty operation of bearings.

  16. Online Condition Monitoring of Bearings to Support Total Productive Maintenance in the Packaging Materials Industry

    Directory of Open Access Journals (Sweden)

    Jovan Gligorijevic

    2016-03-01

    Full Text Available The packaging materials industry has already recognized the importance of Total Productive Maintenance as a system of proactive techniques for improving equipment reliability. Bearing faults, which often occur gradually, represent one of the foremost causes of failures in the industry. Therefore, detection of their faults in an early stage is quite important to assure reliable and efficient operation. We present a new automated technique for early fault detection and diagnosis in rolling-element bearings based on vibration signal analysis. Following the wavelet decomposition of vibration signals into a few sub-bands of interest, the standard deviation of obtained wavelet coefficients is extracted as a representative feature. Then, the feature space dimension is optimally reduced to two using scatter matrices. In the reduced two-dimensional feature space the fault detection and diagnosis is carried out by quadratic classifiers. Accuracy of the technique has been tested on four classes of the recorded vibrations signals, i.e., normal, with the fault of inner race, outer race, and ball operation. The overall accuracy of 98.9% has been achieved. The new technique can be used to support maintenance decision-making processes and, thus, to increase reliability and efficiency in the industry by preventing unexpected faulty operation of bearings.

  17. Infant Milk Formulas: Effect of Storage Conditions on the Stability of Powdered Products towards Autoxidation

    Directory of Open Access Journals (Sweden)

    Stefania Cesa

    2015-09-01

    Full Text Available Thirty samples of powdered infant milk formulas containing polyunsaturated fatty acids (PUFAs have been stored at four different temperatures (20, 28, 40 and 55 °C and periodically monitored for their malondialdehyde (MDA content up to one year. MDA levels ranged between 250 and 350 ng/kg in sealed samples with a maximum of 566 ng/kg in samples stored at 28 °C for three weeks after opening of their original packages, previously maintained for ten months at 20 °C. Sample stored at 40° and 55 °C were also submitted to CIE (Commission Internationale de l’Eclairage colorimetric analysis, since color is the first sensorial property that consumers may evaluate. Overall, the results demonstrated a good stability of PUFA-enriched infant milk formulas in terms of MDA content. However, some care has to be paid when these products are not promptly consumed and stored for a long time after first opening.

  18. Effect of culture conditions on lipase production by Fusarium solani in batch fermentation.

    Science.gov (United States)

    Maia, M M; Heasley, A; Camargo de Morais, M M; Melo, E H; Morais, M A; Ledingham, W M; Lima Filho, J L

    2001-01-01

    Lipase (Glycerol ester hydrolase EC 3.1.1.3.) from a Brazilian strain of Fusarium solani FSI has been investigated. The effect of different carbon sources and trace elements added to basal medium was observed with the aim of improving enzyme production. Lipase specific activity was highest (0.45 U mg(-1)) for sesame oil. When this medium was supplemented with trace elements using olive oil, corn oil and sesame oil the lipase specific activity increased to 0.86, 1.89 and 1.64 U mg(-1), respectively, after 96 h cultivation without any considerable biomass increase. The Km of this lipase using pNPP (p-nitrophenylpalmitate) as substrate, was 1.8 mM with a Vmax of 1.7 micromol min(-1) mg protein(-1). Lipase activity increased in the presence of increasing concentrations of hexane and toluene. In contrast, incubation of this enzyme with water-soluble solvents decreased its activity after 10% concentration (v/v) of the solvent. The lipase activity was stable below 35 degrees C but above this temperature activity losses were observed.

  19. Condition-Based Maintenance Strategy for Production Systems Generating Environmental Damage

    Directory of Open Access Journals (Sweden)

    L. Tlili

    2015-01-01

    Full Text Available We consider production systems which generate damage to environment as they get older and degrade. The system is submitted to inspections to assess the generated environmental damage. The inspections can be periodic or nonperiodic. In case an inspection reveals that the environmental degradation level has exceeded the critical level U, the system is considered in an advanced deterioration state and will have generated significant environmental damage. A corrective maintenance action is then performed to renew the system and clean the environment and a penalty has to be paid. In order to prevent such an undesirable situation, a lower threshold level L is considered to trigger a preventive maintenance action to bring back the system to a state as good as new at a lower cost and without paying the penalty. Two inspection policies are considered (periodic and nonperiodic. For each one of them, a mathematical model and a numerical procedure are developed to determine simultaneously the preventive maintenance (PM threshold L∗ and the inspection sequence which minimize the average long-run cost per time unit. Numerical calculations are performed to illustrate the proposed maintenance policies and highlight their main characteristics with respect to relevant input parameters.

  20. Biodiesel production from Spirulina microalgae feedstock using direct transesterification near supercritical methanol condition.

    Science.gov (United States)

    Mohamadzadeh Shirazi, Hamed; Karimi-Sabet, Javad; Ghotbi, Cyrus

    2017-09-01

    Microalgae as a candidate for production of biodiesel, possesses a hard cell wall that prevents intracellular lipids leaving out from the cells. Direct or in situ supercritical transesterification has the potential for destruction of microalgae hard cell wall and conversion of extracted lipids to biodiesel that consequently reduces the total energy consumption. Response surface methodology combined with central composite design was applied to investigate process parameters including: Temperature, Time, Methanol-to-dry algae, Hexane-to-dry algae, and Moisture content. Thirty-two experiments were designed and performed in a batch reactor, and biodiesel efficiency between 0.44% and 99.32% was obtained. According to fatty acid methyl ester yields, a quadratic experimental model was adjusted and the significance of parameters was evaluated using analysis of variance (ANOVA). Effects of single and interaction parameters were also interpreted. In addition, the effect of supercritical process on the ultrastructure of microalgae cell wall using scanning electron spectrometry (SEM) was surveyed. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Optimization of Production Conditions for Protoplasts and Polyethylene Glycol-Mediated Transformation of Gaeumannomyces tritici.

    Science.gov (United States)

    Wang, Mei; Zhang, Jie; Wang, Lanying; Han, Lirong; Zhang, Xing; Feng, Juntao

    2018-05-24

    Take-all, caused by Gaeumannomyces tritici , is one of the most important wheat root diseases worldwide, as it results in serious yield losses. In this study, G. tritici was transformed to express the hygromycin B phosphotransferase using a combined protoplast and polyethylene glycol (PEG)-mediated transformation technique. Based on a series of single-factor experimental results, three major factors-temperature, enzyme lysis time, and concentration of the lysing enzyme-were selected as the independent variables, which were optimized using the response surface methodology. A higher protoplast yield of 9.83 × 10⁷ protoplasts/mL was observed, and the protoplast vitality was also high, reaching 96.27% after optimization. Protoplasts were isolated under the optimal conditions, with the highest transformation frequency (46⁻54 transformants/μg DNA). Polymerase chain reaction and Southern blotting detection indicated that the genes of hygromycin phosphotransferase were successfully inserted into the genome of G. tritici . An optimised PEG-mediated protoplast transformation system for G. tritici was established. The techniques and procedures described will lay the foundation for establishing a good mutation library of G. tritici and could be used to transform other fungi.

  2. Fission product aerosol removal test by containment spray under accident management conditions (3)

    International Nuclear Information System (INIS)

    Watanabe, Atsushi; Nagasaka, Hideo; Yokobori, Seiichi; Akinaga, Makoto

    2000-01-01

    In order to demonstrate the effective FP aerosol removal by containment spray under Japanese AM conditions, two system integral tests and two separate effect tests were carried out using a full-height simulation test facility. In case of PWR LOCA, aerosol concentration in the upper containment vessel decreased even under low spray flow rate. In case of BWR LOCA with water injection into RPV, the aerosol concentration in the entire vessel also decreased rapidly after aerosol supply stopping. In both cases, the removal rate estimated from the NUREG-1465 was coincided with test results. The aerosol washing effect by spray was confirmed to be predominant by conducting suppression chamber isolation test. It turned out that the effect of aerosol solubility and density on aerosol removal by spray was quite small by conducting insoluble aerosol injection test. After the modification of aerosol removal model by the spray and hygroscopic aerosol model in original MELCOR 1.8.4, calculated aerosol concentration transient in the containment vessel agreed well with the test data. (author)

  3. Productive and quality characteristics of soybean in agroecological conditions of Sombor, Serbia

    Directory of Open Access Journals (Sweden)

    Popović Vera

    2013-01-01

    Full Text Available The objective of this paper was to examine and present the results of soybean grain yield, protein and oil content during a two-year period (2009 to 2010 in agro-ecological conditions of Sombor, Serbia. Data were statistically analyzed by analysis of variance, using the method of two factorial trials (variety, year. LSD was used to compare means for significant differences. Significantly higher yields were recorded in 2010 compared to 2009. Average yield amounted to 4,196 kg ha-1 for analyzed soybean varieties, and ranged from 2,889 kg ha-1 (2009 to 5,503 kg ha-1 (2010. The year 2010 was favourable for protein synthesis. The highest protein content was achieved by Bečejka variety (38.38% and Proteinka variety (38.33% in 2010. Proteinka had statistically significantly higher average protein content compared to other tested varieties in the two-year average. The tested soybean varieties had average oil content of 20.93%. The highest oil content was found in Sava variety (23.03% in 2009. Statistically significantly higher oil content was recorded in 2009 compared to 2010, while variety and their interactions had statistically significant effect (p <0.05, p <0.01 on yield and grain quality of soybean. Irrigation and appropriate cultural practices are the only measures that could mitigate limitation factors and increase yields.

  4. THE WILLINGNESS OF TEACHERS AS A CONDITION FOR PRODUCTIVE INNOVATIVE EDUCATIONAL ACTIVITIES FOR YOUNGER STUDENTS

    Directory of Open Access Journals (Sweden)

    Maria Ivanchuk

    2017-03-01

    Full Text Available The article is analyzed approaches and defined the essence of readiness of teachers to innovative educational activities in the primary education. The meanings of “innovative educational activity”, “the willingness of students to innovative educational activities” are clarified. The essence and role of motivational, cognitive, creative and incentive-praxeology components of this readiness is analysed. Identified, theoretically grounded and experimentally tested pedagogical conditions of formation of readiness of students to innovative educational activities with younger students in the process of pedagogical practice. Developed a program of optimization of innovative educational activities with younger students in the process of pedagogical practice, which covers a system of structured types. The tests showed the efficiency performance of different types of individual and group work with students (experience with innovative educational technologies and their implementation; discussion of issues of innovation in education; implementation of dialogue forms of communication between the actors of the educational process, the formation of aspirations, the desire to highlight the goals, objectives, build a plan of its own search of innovative work; develop skills to make the transition from algorithmisation, stereotypical techniques to subjectively new etc.. Methodical recommendations are offered for improving the preparation of students for innovative educational activities with the younger students.

  5. Effect of electrolysis condition of zinc powder production on zinc-silver oxide battery operation

    International Nuclear Information System (INIS)

    Mojtahedi, M.; Goodarzi, M.; Sharifi, B.; Vahdati Khaki, J.

    2011-01-01

    A research conducted to produce zinc powder through electrolysis of alkaline solutions by using various concentrations of KOH and zincate in the bath. Different current densities were applied for each concentration and then, morphological changes of Zn powder batches were examined by scanning electron microscopy. Afterward, an anode electrode was produced from each pack of powder. Thirty-six Zn-AgO battery cells were prepared totally. Discharge parameters of the cells were examined and time-voltage curves were analyzed. Discharge times were investigated for various conditions of Zn deposition and the proper terms were suggested. It has been seen that increase of KOH concentration and decrease of zincate ion in the bath solution will change the zinc morphology and increase the resultant battery discharge time. The longest time of discharge, before reduction of cell voltage to 1.25 V, was 7.91 min. This result was obtained for Zn powder produced in zincate concentration of 0.5 M, KOH concentration of 11 M and current density of 2500 A/m 2 .

  6. Productive and qualitative evaluation of onion cultivars under agro-climatic conditions of faisalabad

    International Nuclear Information System (INIS)

    Mushtaq, S.; Amjad, M.; Ziaf, K.; Cheema, K.L.

    2013-01-01

    Onion varieties are very specific in their photoperiod and vernalization requirements and therefore vary for yield, yield related traits and bolting in a specific agro-climate. Therefore, performance of nineteen onion varieties for these traits was evaluated at Vegetable Research Institute, Faisalabad. The maximum bolting percentage was recorded in Desi Red (46.67%) that indicates less vernalization requirement of this variety while it was the minimum in Faisal Red and VRIO-6 (13.33%). The cultivar Phulkara produced larger size bulbs (73.22 mm diameter) as well as highest yield (21.90 t ha/sup -1/) and bulb to neck diameter ratio (6.75). Similarly, minimum weight loss during curing was observed in Desi Red (4.64%), Pusa Red (4.76%) and Phulkara (4.83%), indicating higher dry matter contents while maximum weight loss (6%) was recorded in VRIO-6. Overall results revealed that both Phulkara and Desi Red are excellent for processing while Dark Red for cooking purpose under agro-climatic conditions of Faisalabad. (author)

  7. Enhanced production of natural yellow pigments from Monascus purpureus by liquid culture: The relationship between fermentation conditions and mycelial morphology.

    Science.gov (United States)

    Lv, Jun; Zhang, Bo-Bo; Liu, Xiao-Dong; Zhang, Chan; Chen, Lei; Xu, Gan-Rong; Cheung, Peter Chi Keung

    2017-10-01

    Natural yellow pigments produced by submerged fermentation of Monascus purpureus have potential economic value and application in the food industry. In the present study, the relationships among fermentation conditions (in terms of pH and shaking/agitation speed), mycelial morphology and the production of Monascus yellow pigments were investigated in both shake-flask and scale-up bioreactor experiments. In the shake-flask fermentation, the highest yield of the Monascus yellow pigments was obtained at pH 5.0 and a shaking speed of 180 rpm. Microscopic images revealed that these results were associated with the formation of freely dispersed small mycelial pellets with shorter, thicker and multi-branched hyphae. Further investigation indicated that the hyphal diameter was highly correlated with the biosynthesis of the Monascus yellow pigments. In a scaled-up fermentation experiment, the yield of yellow pigments (401 U) was obtained in a 200-L bioreactor, which is the highest yield to the best of our knowledge. The present findings can advance our knowledge on the conditions used for enhancing the production of Monascus yellow pigments in submerged fermentation and facilitate large-scale production of these natural pigments. Copyright © 2017 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

  8. Bioprocessing of wheat bran for the production of lignocellulolytic enzyme cocktail by Cotylidia pannosa under submerged conditions

    Science.gov (United States)

    Sharma, Deepika; Garlapat, Vijay Kumar; Goel, Gunjan

    2016-01-01

    ABSTRACT Characterization and production of efficient lignocellulytic enzyme cocktails for biomass conversion is the need for biofuel industry. The present investigation reports the modeling and optimization studies of lignocellulolytic enzyme cocktail production by Cotylidia pannosa under submerged conditions. The predominant enzyme activities of cellulase, xylanase and laccase were produced in the cocktail through submerged conditions using wheat bran as a substrate. A central composite design approach was utilized to model the production process using temperature, pH, incubation time and agitation as input variables with the goal of optimizing the output variables namely cellulase, xylanase and laccase activities. The effect of individual, square and interaction terms on cellulase, xylanase and laccase activities were depicted through the non-linear regression equations with significant R2 and P-values. An optimized value of 20 U/ml, 17 U/ml and 13 U/ml of cellulase, xylanase and laccase activities, respectively, were obtained with a media pH of 5.0 in 77 h at 31C, 140 rpm using wheatbran as a substrate. Overall, the present study introduces a fungal strain, capable of producing lignocellulolytic enzyme cocktail for subsequent applications in biofuel industry. PMID:26941214

  9. Optimization of the marinating conditions of cassava fish (Pseudotolithus sp.) fillet for Lanhouin production through application of Doehlert experimental design.

    Science.gov (United States)

    Kindossi, Janvier Mêlégnonfan; Anihouvi, Victor Bienvenu; Vieira-Dalodé, Générose; Akissoé, Noël Houédougbé; Hounhouigan, Djidjoho Joseph

    2016-03-01

    Lanhouin is a traditional fermented salted fish made from the spontaneous and uncontrolled fermentation of whole salted cassava fish (Pseudotolithus senegalensis) mainly produced in the coastal regions of West Africa. The combined effects of NaCl, citric acid concentration, and marination time on the physicochemical and microbiological characteristics of the fish fillet used for Lanhouin production were studied using a Doehlert experimental design with the objective of preserving its quality and safety. The marination time has significant effects on total viable and lactic acid bacteria counts, and NaCl content of the marinated fish fillet while the pH was significantly affected by citric acid concentration and marination duration with high regression coefficient R (2) of 0.83. The experiment showed that the best conditions for marination process of fish fillet were salt ratio 10 g/100 g, acid citric concentration 2.5 g/100 g, and marination time 6 h. These optimum marinating conditions obtained present the best quality of marinated flesh fish leading to the safety of the final fermented product. This pretreatment is necessary in Lanhouin production processes to ensure its safety quality.

  10. Environmental Growing Conditions in Five Production Systems Induce Stress Response and Affect Chemical Composition of Cocoa (Theobroma cacao L.) Beans.

    Science.gov (United States)

    Niether, Wiebke; Smit, Inga; Armengot, Laura; Schneider, Monika; Gerold, Gerhard; Pawelzik, Elke

    2017-11-29

    Cocoa beans are produced all across the humid tropics under different environmental conditions provided by the region but also by the season and the type of production system. Agroforestry systems compared to monocultures buffer climate extremes and therefore provide a less stressful environment for the understory cocoa, especially under seasonally varying conditions. We measured the element concentration as well as abiotic stress indicators (polyamines and total phenolic content) in beans derived from five different production systems comparing monocultures and agroforestry systems and from two harvesting seasons. Concentrations of N, Mg, S, Fe, Mn, Na, and Zn were higher in beans produced in agroforestry systems with high stem density and leaf area index. In the dry season, the N, Fe, and Cu concentration of the beans increased. The total phenolic content increased with proceeding of the dry season while other abiotic stress indicators like spermine decreased, implying an effect of the water availability on the chemical composition of the beans. Agroforestry systems did not buffer the variability of stress indicators over the seasons compared to monocultures. The effect of environmental growing conditions on bean chemical composition was not strong but can contribute to variations in cocoa bean quality.

  11. High throughput nanostructure-initiator mass spectrometry screening of microbial growth conditions for maximal β-glucosidase production.

    Science.gov (United States)

    Cheng, Xiaoliang; Hiras, Jennifer; Deng, Kai; Bowen, Benjamin; Simmons, Blake A; Adams, Paul D; Singer, Steven W; Northen, Trent R

    2013-01-01

    Production of biofuels via enzymatic hydrolysis of complex plant polysaccharides is a subject of intense global interest. Microbial communities are known to express a wide range of enzymes necessary for the saccharification of lignocellulosic feedstocks and serve as a powerful reservoir for enzyme discovery. However, the growth temperature and conditions that yield high cellulase activity vary widely, and the throughput to identify optimal conditions has been limited by the slow handling and conventional analysis. A rapid method that uses small volumes of isolate culture to resolve specific enzyme activity is needed. In this work, a high throughput nanostructure-initiator mass spectrometry (NIMS)-based approach was developed for screening a thermophilic cellulolytic actinomycete, Thermobispora bispora, for β-glucosidase production under various growth conditions. Media that produced high β-glucosidase activity were found to be I/S + glucose or microcrystalline cellulose (MCC), Medium 84 + rolled oats, and M9TE + MCC at 45°C. Supernatants of cell cultures grown in M9TE + 1% MCC cleaved 2.5 times more substrate at 45°C than at all other temperatures. While T. bispora is reported to grow optimally at 60°C in Medium 84 + rolled oats and M9TE + 1% MCC, approximately 40% more conversion was observed at 45°C. This high throughput NIMS approach may provide an important tool in discovery and characterization of enzymes from environmental microbes for industrial and biofuel applications.

  12. High throughput nanostructure-initiator mass spectrometry screening of microbial growth conditions for maximal β-glucosidase production

    Directory of Open Access Journals (Sweden)

    Xiaoliang eCheng

    2013-12-01

    Full Text Available Production of biofuels via enzymatic hydrolysis of complex plant polysaccharides is a subject of intense global interest. Microbial communities are known to express a wide range of enzymes necessary for the saccharification of lignocellulosic feedstocks and serve as a powerful reservoir for enzyme discovery. However, the growth temperature and conditions that yield high cellulase activity vary widely, and the throughput to identify optimal conditions has been limited by the slow handling and conventional analysis. A rapid method that uses small volumes of isolate culture to resolve specific enzyme activity is needed. In this work, a high throughput nanostructure-initiator mass spectrometry (NIMS based approach was developed for screening a thermophilic cellulolytic actinomycete, Thermobispora bispora, for β-glucosidase production under various growth conditions. Media that produced high β-glucosidase activity were found to be I/S + glucose or microcrystalline cellulose (MCC, Medium 84 + rolled oats, and M9TE + MCC at 45 °C. Supernatants of cell cultures grown in M9TE + 1% MCC cleaved 2.5 times more substrate at 45 °C than at all other temperatures. While T. bispora is reported to grow optimally at 60 °C in Medium 84 + rolled oats and M9TE + 1% MCC, approximately 40% more conversion was observed at 45 °C. This high throughput NIMS approach may provide an important tool in discovery and characterization of enzymes from environmental microbes for industrial and biofuel applications.

  13. Influence of culture conditions and medium composition on the production of antibacterial compounds by marine Serratia sp. WPRA3.

    Science.gov (United States)

    Jafarzade, Mahtab; Yahya, Nur Ain; Shayesteh, Fatemeh; Usup, Gires; Ahmad, Asmat

    2013-06-01

    This study was undertaken to investigate the influence of culture conditions and medium components on production of antibacterial compounds by Serratia sp. WPRA3 (JX020764) which was isolated from marine water of Port Dickson, Malaysia. Biochemical, morphological, and molecular characteristics suggested that the isolate is a new candidate of the Serratia sp. The isolate showed strong antimicrobial activity against fungi, Gram-negative and Gram-positive bacteria. This bacterium exhibited optimum antibacterial compounds production at 28°C, pH 7 and 200 rev/min aeration during 72 h of incubation period. Highest antibacterial activity was obtained when sodium chloride (2%), yeast extract (0.5%), and glucose concentration (0.75%) were used as salt, nitrogen, and carbon sources respectively. Different active fractions were obtained by Thin-Layer Chromatography (TLC) and Flash Column Chromatography (FCC) from ethyl acetate crude extracts namely OCE and RCE in different culture conditions, OCE (pH 5, 200 rev/min) and RCE (pH 7/without aeration). In conclusion, the results suggested different culture conditions have a significant impact on the types of secondary metabolites produced by the bacterium.

  14. Optimization of liquid-state fermentation conditions for the glyphosate degradation enzyme production of strain Aspergillus oryzae by ultraviolet mutagenesis.

    Science.gov (United States)

    Fu, Gui-Ming; Li, Ru-Yi; Li, Kai-Min; Hu, Ming; Yuan, Xiao-Qiang; Li, Bin; Wang, Feng-Xue; Liu, Cheng-Mei; Wan, Yin

    2016-11-16

    This study aimed to obtain strains with high glyphosate-degrading ability and improve the ability of glyphosate degradation enzyme by the optimization of fermentation conditions. Spore from Aspergillus oryzae A-F02 was subjected to ultraviolet mutagenesis. Single-factor experiment and response surface methodology were used to optimize glyphosate degradation enzyme production from mutant strain by liquid-state fermentation. Four mutant strains were obtained and named as FUJX 001, FUJX 002, FUJX 003, and FUJX 004, in which FUJX 001 gave the highest total enzyme activity. Starch concentration at 0.56%, GP concentration at 1,370 mg/l, initial pH at 6.8, and temperature at 30°C were the optimum conditions for the improved glyphosate degradation endoenzyme production of A. oryzae FUJX 001. Under these conditions, the experimental endoenzyme activity was 784.15 U/100 ml fermentation liquor. The result (784.15 U/100 ml fermentation liquor) was approximately 14-fold higher than that of the original strain. The result highlights the potential of glyphosate degradation enzyme to degrade glyphosate.

  15. Clinical course of brain stroke in the persons exposed to ionizing radiation under the production conditions

    International Nuclear Information System (INIS)

    Bouchmanov, A.

    2000-01-01

    The purpose was to study the risk factors and clinical course of brain strokes in professionally exposed workers being employed in plutonium production in comparison with a control group. The method and materials of study -clinical supervision and clinical database creation on 162 cases of brain stroke (128 males and 34 females) developed among professionally exposed workers. Age of patient varied from 21 to 68 years (in average -51.6 y.). The control group consisted of patients with the same diagnosis, worked on the same enterprise, but non-exposed to radiation. Data on the totally accumulated dose of external gamma radiation were received on the base of the individual dosimeters (from 0.1 cSv to 52 cSv, in average about 13 cSv); the plutonium-239 body content was estimated accordingly to the level of urine radionuclide excretion (from 0.4 kBq to 1.6 kBq, in average about 0.33 kBq). Muscle's hypertinsion and pathological great-toe reflexes in paretic legs and hands, hemianopsia, impressive and ataxic aphasia prevailed in the patients with ischemic brain strokes in system of internal carotid artery, exposed to radiation. The changes of muscle's tension, ataxia and nystagmus were marked more often in the professionals with ischemic brain strokes in system of vertebrobasilar artery. The illness proceeded more easy and with smaller frequency of frustration of consciousness and algesthesia, irrespective of a type ischemic brain strokes in the people exposed to ionizing radiation, than in patients of non-irradiated group. It was found that the arterial hypertension appeared to be the main risk factor for the brain stroke in both groups of patients (in 81.48% and 91.15% of cases). There was no marked differences in significance of risk factors and in main clinical parameters of various types of ischemic brain strokes among the patients professionally exposed to radiation in comparison with a control group. (author)

  16. Responses of fresh-cut products of four mango cultivars under two different storage conditions.

    Science.gov (United States)

    Sharma, Sonu; Rao, Tadapaneni Venkata Ramana

    2017-05-01

    Due to availability of minimally processed products, the consumption of fresh produce has increased over recent years. The present study has been undertaken with the objective of screening of four mango cultivars ('Kesar', 'Rajapuri', 'Totapuri' and 'Ladvo') for evaluating the consequences of minimal processing on their quality attributes under storage at two different temperatures (5 ± 1 °C, 95% RH and 10 ± 1 °C, 87% RH) up to 12 days. The result of the study revealed significant impacts of low temperature storage on the quality parameters of fresh-cut mango cultivars. The evaluated bioactive compounds such as total phenolics, vitamin C and carotenoids were better retained in the samples stored at 5 °C as compared with that of 10 °C. Moreover, the storage of fresh-cut mango cultivars at 5 °C showed lower water loss and microbial contamination. Sensory analyses revealed that the storage of fresh-cut mango cultivars at 10 °C influenced overall acceptability due to changes in their visual perception, though taste, odor and firmness were less affected. This study revealed a significant variation in the storability of fresh-cut mango cultivars with respect to the storage temperature. Among currently studied four cultivars of mango, slices of 'Totapuri' showed comparatively the least change in color, firmness and sensory properties during storage at 5 and 10 °C and it can be a potential cultivar for fresh-cut processing.

  17. Technical chemical conditions for alcohol production from beet-sugar molasses and starchy raw mateials

    Energy Technology Data Exchange (ETDEWEB)

    Zabrodskii, A G; Vitkovskaya, V A; Orlovskii, Ya K

    1963-01-01

    The industrial conditions for the combined use of these raw materials varies considerably from those for separate application. Calculation formulas are developed, and example calculations are shown, for yields, purity coefficient, conversion values after saccharification of potato and grain starches, and mash concentrations prior to mixing with molasses mash for optimum yields. Of high importance is the acidity adjustment prior to fermentation. The molasses mash joining the saccharified starch mash is so adjusted as to produce a final mixture of pH 5.3 to 5.6, and to titrate with saccharified grain starch 0.25 to 0.3/sup 0/ acidity, and with potato starch 0.3 to 0.4/sup 0/. The reduction of molasses alkalinity by 1/sup 0/ (1/sup 0/ alkalinity corresponds during titration to 1 cc. of N H/sub 2/SO/sub 4/ in 100 g. molasses) for 1 ton requires 270 cc. H/sub 2/SO/sub 4/ (monohydrate) or 770 cc. HCl (sp. gr. 1.19). For acidifying 1 m./sup 3/ molasses mash by 0.1/sup 0/, 140 cc. H/sub 2/SO/sub 4/ or 400 cc. HCl are needed. Molasses are diluted and sterilized at 85 to 90/sup 0/ for 30 to 45 minutes, cooled down, and adjusted with cold water to the required sugar content. Yeast is added to provide a concentration of 80 to 90 million/cc. in grain- or potato-starch mash prior to admixture of molasses mash; it reaches 130 to 140 million cc. after completion of admixture. The molasses mash is added after the starch mash has fermented down to 10 to 11% and the addition takes 8 to 10 hours for a total fermentation time of 48 hours.

  18. APPLICATON OF MYCORRIZA ON PLANTING MEDIA OF TWO TOMATO VARIETIES TO INCREASEVEGETABLE PRODUCTIVITY IN DROUGHT CONDITION

    Directory of Open Access Journals (Sweden)

    Paramita Cahyaningrum Kuswandi

    2016-04-01

    Full Text Available A method used for the development of dry areas/marginal lands is the improvement of soil structure and addition to the media to support the growth and development of crops. Tomatoes have the potential to be developed in marginal lands due to its high nutrition, high in demand and easy to be cultivated. One of the method used to improve planting media is the addition of microorganism such as mycorrhiza which can help the absorbtion of water and nutrition for plants. The interval of irrigation is used as a simulation of drought. This research aim was to observe the effect of mycorrhiza in the soil on the growth and development of tomato with several treatments of irrigation. The method used was the addition of 4 g of mycorrhiza  per polybag (size 30x30 cm2, using Complete Randomized Design. There were 6 combinations of treatments. The treatments were : 3 interval of irrigation (every day, every 7 days and every 14 days, and 2 treatments of mycorrhiza (0 g and 4 g. There were 3 repetition for each combination of treatments. The results showed that the addition of mycorrhiza can increase significantly plant fresh and dry weight and also root length. The difference in plant height, number of branches, number of leaves, plant growth rate and percentage of infection were caused by the difference in irrigation interval. The difference in the varieties used also contribute to a difference in the percentage of infection. Further research must be made on the effect of mycorrhiza with addition of inorganic fertilizer to increase the growth and development of tomato plants in water stressed condition. Keywords:   mycorriza, tomato, draught simulation

  19. Clinical course of brain stroke in the persons exposed to ionizing radiation under the production conditions

    Energy Technology Data Exchange (ETDEWEB)

    Bouchmanov, A. [State Research Center of Russia, Moscow (Russian Federation). Inst. of Biophysics

    2000-05-01

    The purpose was to study the risk factors and clinical course of brain strokes in professionally exposed workers being employed in plutonium production in comparison with a control group. The method and materials of study -clinical supervision and clinical database creation on 162 cases of brain stroke (128 males and 34 females) developed among professionally exposed workers. Age of patient varied from 21 to 68 years (in average -51.6 y.). The control group consisted of patients with the same diagnosis, worked on the same enterprise, but non-exposed to radiation. Data on the totally accumulated dose of external gamma radiation were received on the base of the individual dosimeters (from 0.1 cSv to 52 cSv, in average about 13 cSv); the plutonium-239 body content was estimated accordingly to the level of urine radionuclide excretion (from 0.4 kBq to 1.6 kBq, in average about 0.33 kBq). Muscle's hypertinsion and pathological great-toe reflexes in paretic legs and hands, hemianopsia, impressive and ataxic aphasia prevailed in the patients with ischemic brain strokes in system of internal carotid artery, exposed to radiation. The changes of muscle's tension, ataxia and nystagmus were marked more often in the professionals with ischemic brain strokes in system of vertebrobasilar artery. The illness proceeded more easy and with smaller frequency of frustration of consciousness and algesthesia, irrespective of a type ischemic brain strokes in the people exposed to ionizing radiation, than in patients of non-irradiated group. It was found that the arterial hypertension appeared to be the main risk factor for the brain stroke in both groups of patients (in 81.48% and 91.15% of cases). There was no marked differences in significance of risk factors and in main clinical parameters of various types of ischemic brain strokes among the patients professionally exposed to radiation in comparison with a control group. (author)

  20. Excess heat production in Pd/D during periodic pulse discharge current in various conditions

    Energy Technology Data Exchange (ETDEWEB)

    Karabut, A.B. [FSUE ' LUCH' , 24 Zheleznodorozhnaya St., Podolsk, Moscow Region 142100 (Russian Federation)

    2006-07-01

    Experimental date from low-energy nuclear reactions (LENR) in condensed media are presented. The nuclear reactions products were found in solid cathode media used in glow discharge. Apparently, the nuclear reactions were initiated when bombarding the cathode surface by plasma ions with the energy of 1.0 - 2.0 keV. Excess heat from a high current glow discharge reaction in D{sub 2}, Xe, and Kr using cathodes already charged with preliminary deuterium-charged Pd and Ti cathode samples are given. Excess heat up to 10-15 W and efficiency up to 130% were recorded under the experiments for Pd cathode samples in D{sub 2} discharge. Excess heat up to 5 W and efficiency up to 150% were recorded for Pd cathodes that were charged with deuterium before the run, in Xe and Kr discharges. At the same time excess heat was not observed for pure Pd cathode samples in Xe and Kr discharges. The formation of impurity nuclides ({sup 7}Li, {sup 13}C, {sup 15}N, {sup 20}Ne, {sup 29}Si, {sup 44}Ca, {sup 48}Ca, {sup 56}Fe, {sup 57}Fe, {sup 59}Co, {sup 64}Zn, {sup 66}Zn, {sup 75}As, {sup 107}Ag, {sup 109}Ag, {sup 110}Cd, {sup 111}Cd, {sup 112}Cd, {sup 114}Cd and {sup 115}In) with 'the efficiency up to 10{sup 13} at./s was recorded. The isotopic ratios of these new nuclides were quite different from the natural ratios. Soft X-ray radiation from the solid-state cathode with the intensity up to 0.01 Gy/s was recorded in experiments with discharges in H{sub 2}, D{sub 2}, Ar, Xe, and Kr. The X-ray radiation was observed in bursts of up to 10{sup 6} photons, with up to 10{sup 5} bursts per second while the discharge was formed and within 100 ms after turning off the discharge current. The results of the X-ray radiation registration showed that the excited energy levels have a lifetime up to 100 ms or more, and the energy of 1.2 - 2.5 keV. A possible mechanism for producing excess heat and nuclear transmutation reactions in the solid medium with the excited energy levels is considered.

  1. Excess heat production in Pd/D during periodic pulse discharge current in various conditions

    International Nuclear Information System (INIS)

    Karabut, A.B.

    2006-01-01

    Experimental date from low-energy nuclear reactions (LENR) in condensed media are presented. The nuclear reactions products were found in solid cathode media used in glow discharge. Apparently, the nuclear reactions were initiated when bombarding the cathode surface by plasma ions with the energy of 1.0 - 2.0 keV. Excess heat from a high current glow discharge reaction in D 2 , Xe, and Kr using cathodes already charged with preliminary deuterium-charged Pd and Ti cathode samples are given. Excess heat up to 10-15 W and efficiency up to 130% were recorded under the experiments for Pd cathode samples in D 2 discharge. Excess heat up to 5 W and efficiency up to 150% were recorded for Pd cathodes that were charged with deuterium before the run, in Xe and Kr discharges. At the same time excess heat was not observed for pure Pd cathode samples in Xe and Kr discharges. The formation of impurity nuclides ( 7 Li, 13 C, 15 N, 20 Ne, 29 Si, 44 Ca, 48 Ca, 56 Fe, 57 Fe, 59 Co, 64 Zn, 66 Zn, 75 As, 107 Ag, 109 Ag, 110 Cd, 111 Cd, 112 Cd, 114 Cd and 115 In) with 'the efficiency up to 10 13 at./s was recorded. The isotopic ratios of these new nuclides were quite different from the natural ratios. Soft X-ray radiation from the solid-state cathode with the intensity up to 0.01 Gy/s was recorded in experiments with discharges in H 2 , D 2 , Ar, Xe, and Kr. The X-ray radiation was observed in bursts of up to 10 6 photons, with up to 10 5 bursts per second while the discharge was formed and within 100 ms after turning off the discharge current. The results of the X-ray radiation registration showed that the excited energy levels have a lifetime up to 100 ms or more, and the energy of 1.2 - 2.5 keV. A possible mechanism for producing excess heat and nuclear transmutation reactions in the solid medium with the excited energy levels is considered

  2. Influence of synoptic condition and holiday effects on VOCs and ozone production in the Yangtze River Delta region, China

    Science.gov (United States)

    Xu, Zhengning; Huang, Xin; Nie, Wei; Chi, Xuguang; Xu, Zheng; Zheng, Longfei; Sun, Peng; Ding, Aijun

    2017-11-01

    Both anthropogenic emission and synoptic conditions play important roles in ozone (O3) formation and accumulation. In order to understand the influence of synoptic condition and holiday effects on ozone production in the Yangtze River Delta region, China, concentrations of speciated volatile organic compounds (VOCs) and O3 as well as other relevant trace gases were simultaneously measured at the Station for Observing Regional Processes of the Earth System (SORPES) in Nanjing around the National Day holidays of China in 2014, which featured substantial change of emissions and dominated by typical anti-cyclones. Different groups of VOC species and their chemical reactivities were comprehensively analyzed. We observed clear diurnal variations of short alkenes during the measurement period, considerable amount of short alkenes were observed during night (more than 10 ppb) while almost no alkenes were measured during daytime, which might be attributed to different chemical processes. The obvious enhancement of the VOC tracers during the National Day holidays (Oct. 1st-Oct. 7th) indicated that the holiday effect strongly influenced the distribution of VOC profile and chemical reactivity in the atmosphere. At the same time, two meso-scale anticyclone processes were also observed during the measurement period. The synoptic condition contributed to the accumulation of VOCs and other precursors, which consequently impacted the ozone production in this region. The integrated influence of synoptic and holiday effects was also analyzed with an Observation Based Model (OBM) based on simplified MCM (Master Chemical Mechanism) chemical mechanism. The calculated relative increment reactivity (RIR) of different VOC groups revealed that during the holidays, this region was in VOC-limited regime and the variation of RIR shows a close linkage to the development and elimination of anti-cyclones, indicating an in-negligible contribution of synoptic effect toward ozone production in this

  3. Optimization of flask culture medium and conditions for hyaluronic acid production by a streptococcus equisimilis mutant nc2168

    Directory of Open Access Journals (Sweden)

    Yong-Hao Chen

    2012-12-01

    Full Text Available A mutant designated NC2168, which was selected from wild-type Streptococcus equisimilis CVCC55116by ultraviolet ray combined with60Co-γ ray treatment and does not produce streptolysin, was employed to produce hyaluronic acid (HA. In order to increase the output of HA in a flask, the culture medium and conditions for NC2168 were optimized in this study. The influence of culture medium ingredients including carbon sources, nitrogen sources and metal ions on HA production was evaluated using factional factorial design. The mathematical model, which represented the effect of each medium component and their interaction on the yield of HA, was established by the quadratic rotary combination design and response surface method. The model estimated that, a maximal yield of HA could be obtained when the concentrations of yeast extract, peptone, glucose, and MgSO4 were set at 3 g/100 mL, 2 g/100 mL, 0.5 g/100 mL and 0.15 g/100 mL, respectively. Compared with the values obtained by other runs in the experimental design, the optimized medium resulted in a remarkable increase in the output of HA and the maximum of the predicted HA production was 174.76 mg/L. The model developed was accurate and reliable for predicting the production of HA by NC2168.Cultivation conditions were optimized by an orthogonal experimental design and the optimal conditions were as follows: temperature 33ºC, pH 7.8, agitation speed 200 rpm, medium volume 20 mL.

  4. Modelling 3H and 14C transfer to farm animals and their products under steady state conditions

    International Nuclear Information System (INIS)

    Galeriu, D.; Melintescu, A.; Beresford, N.A.; Crout, N.M.J.; Peterson, R.; Takeda, H.

    2007-01-01

    The radionuclides 14 C and 3 H may both be released from nuclear facilities. These radionuclides are unusual, in that they are isotopes of macro-elements which form the basis of animal tissues, feed and, in the case of 3 H, water. There are few published values describing the transfer of 3 H and 14 C from feed to animal derived food products under steady state conditions. Approaches are described which enable the prediction of 14 C and 3 H transfer parameter values from readily available information on the stable H or C concentration of animal feeds, tissues and milk, water turnover rates, and feed intakes and digestibilities. We recommend that the concentration ratio between feed and animal product activity concentrations be used as it is less variable than the transfer coefficient (ratio between radionuclide activity concentration in animal milk or tissue to the daily intake of a radionuclide)

  5. Fission products and nuclear fuel behaviour under severe accident conditions part 1: Main lessons learnt from the first VERDON test

    Science.gov (United States)

    Pontillon, Y.; Geiger, E.; Le Gall, C.; Bernard, S.; Gallais-During, A.; Malgouyres, P. P.; Hanus, E.; Ducros, G.

    2017-11-01

    This paper describes the first VERDON test performed at the end of September 2011 with special emphasis on the behaviour of fission products (FP) and actinides during the accidental sequence itself. Two other papers discuss in detail the post-test examination results (SEM, EPMA and SIMS) of the VERDON-1 sample. The first VERDON test was devoted to studying UO2 fuel behaviour and fission product releases under reducing conditions at very high temperature (∼2883 K), which was able to confirm the very good performance of the VERDON loop. The fuel sample did not lose its integrity during this test. According to the FP behaviour measured by the online gamma station (fuel sight), the general classification of the FP in relation to their released fraction is very accurate, and the burn-up effect on the release rate is clearly highlighted.

  6. Production of Secondary Metabolites in Extreme Environments: Food- and Airborne Wallemia spp. Produce Toxic Metabolites at Hypersaline Conditions

    DEFF Research Database (Denmark)

    Jančič, Sašo; Frisvad, Jens Christian; Kocev, Dragi

    2016-01-01

    the genome data analysis of W. mellicola (previously W. sebi sensu lato) and W. ichthyophaga revealed a low number of secondary metabolites clusters, a substantial number of secondary metabolites were detected at different conditions. Machine learning analysis of the obtained dataset showed that NaCl has...... of salt or sugar. In relation to food safety, the effect of high salt and sugar concentrations on the production of secondary metabolites by this toxigenic fungus was investigated. The secondary metabolite profiles of 30 strains of the listed species were examined using general growth media, known...... to support the production of secondary metabolites, supplemented with different concentrations of NaCl, glucose and MgCl2. In more than two hundred extracts approximately one hundred different compounds were detected using high-performance liquid chromatography-diode array detection (HPLC-DAD). Although...

  7. Evaluation and prioritization of rice production practices and constraints under temperate climatic conditions using Fuzzy Analytical Hierarchy Process (FAHP

    Directory of Open Access Journals (Sweden)

    Shabir A. Mir

    2016-12-01

    Full Text Available Due to overwhelming complex and vague nature of interactions between multiple factors describing agriculture, Multi-Criteria Decision Making (MCDM methods are widely used from farm to fork to facilitate systematic and transparent decision support, figure out multiple decision outcomes and equip decision maker with confident decision choices in order to choose best alternative. This research proposes a Fuzzy Analytical Hierarchy Process (FAHP based decision support to evaluate and prioritize important factors of rice production practices and constraints under temperate climatic conditions and provides estimate of weightings, which measure relative importance of critical factors of the crop under biotic, abiotic, socio-economic and technological settings. The results envisage that flood, drought, water logging, late sali, temperature and rainfall are important constraints. However, regulating transplantation time; maintaining planting density; providing training to the educated farmers; introducing high productive varieties like Shalimar Rice-1 and Jhelum; better management of nutrients, weeds and diseases are most important opportunities to enhance rice production in the region. Therefore, the proposed system supplements farmers with precise decision information about important rice production practices, opportunities and constraints.

  8. Evaluation and prioritization of rice production practices and constraints under temperate climatic conditions using Fuzzy Analytical Hierarchy Process (FAHP)

    International Nuclear Information System (INIS)

    Mir, S.A.; Padma, T.

    2016-01-01

    Due to overwhelming complex and vague nature of interactions between multiple factors describing agriculture, Multi-Criteria Decision Making (MCDM) methods are widely used from farm to fork to facilitate systematic and transparent decision support, figure out multiple decision outcomes and equip decision maker with confident decision choices in order to choose best alternative. This research proposes a Fuzzy Analytical Hierarchy Process (FAHP) based decision support to evaluate and prioritize important factors of rice production practices and constraints under temperate climatic conditions and provides estimate of weightings, which measure relative importance of critical factors of the crop under biotic, abiotic, socio-economic and technological settings. The results envisage that flood, drought, water logging, late sali, temperature and rainfall are important constraints. However, regulating transplantation time; maintaining planting density; providing training to the educated farmers; introducing high productive varieties like Shalimar Rice-1 and Jhelum; better management of nutrients, weeds and diseases are most important opportunities to enhance rice production in the region. Therefore, the proposed system supplements farmers with precise decision information about important rice production practices, opportunities and constraints.

  9. Evaluation and prioritization of rice production practices and constraints under temperate climatic conditions using Fuzzy Analytical Hierarchy Process (FAHP)

    Energy Technology Data Exchange (ETDEWEB)

    Mir, S.A.; Padma, T.

    2016-07-01

    Due to overwhelming complex and vague nature of interactions between multiple factors describing agriculture, Multi-Criteria Decision Making (MCDM) methods are widely used from farm to fork to facilitate systematic and transparent decision support, figure out multiple decision outcomes and equip decision maker with confident decision choices in order to choose best alternative. This research proposes a Fuzzy Analytical Hierarchy Process (FAHP) based decision support to evaluate and prioritize important factors of rice production practices and constraints under temperate climatic conditions and provides estimate of weightings, which measure relative importance of critical factors of the crop under biotic, abiotic, socio-economic and technological settings. The results envisage that flood, drought, water logging, late sali, temperature and rainfall are important constraints. However, regulating transplantation time; maintaining planting density; providing training to the educated farmers; introducing high productive varieties like Shalimar Rice-1 and Jhelum; better management of nutrients, weeds and diseases are most important opportunities to enhance rice production in the region. Therefore, the proposed system supplements farmers with precise decision information about important rice production practices, opportunities and constraints.

  10. Investigation of the effect of UV-LED exposure conditions on the production of vitamin D in pig skin

    DEFF Research Database (Denmark)

    Barnkob, Line Lundbæk; Argyraki, Aikaterini; Petersen, Paul Michael

    2016-01-01

    study the exposure conditions with ultraviolet (UV) LEDs were systematically investigated in the wavelength range 280-340 nm for achieving optimal vitamin D bio-fortification in pig skin. A wavelength of 296 nm was found to be optimal for vitamin D3 production. The maximum dose of 20 kJ/m2 produced 3.......5-4 µg vitamin D3/cm2 pig skin. Vitamin D3 produced was independent on the combination of time and intensity of the LED source. The increased UV exposure by UV-LEDs may be readily implemented in existing food production facilities, without major modifications to the process or processing equipment......The dietary intake of vitamin D is currently below the recommended intake of 10-20 µg vitamin D/day. Foods with increased content of vitamin D or new products with enhanced vitamin D are warranted. Light-emitting diodes (LEDs) are a potential new resource in food production lines. In the present...

  11. Optimization of fermentation conditions for green pigment production from Bacillus cereus M¹ 16 (MTCC 5521) and its pharmacological application.

    Science.gov (United States)

    Banerjee, D; Mondal, A; Gupta, M; Guha, A K; Ray, L

    2014-01-01

    Optimal culture conditions for the production of green pigment was investigated. The optimal culture condition for the production of an extracellular green pigment by growing Bacillus cereus M(1) 16 (MTCC 5521) in a complex medium containing (g l(-1) ) Peptone-4.0, Beef Extract-9.0, NaCl-7.0, MgSO4 .7H2 O-1.0 and KH2 PO4 -5.0 was as follows pH-7.0 at 30°C for 72 h in a 5 l fermenter. Aeration rate and agitator speed had no effect on the pigment production. Thin layer chromatogram of the pigment extracted from the fermented broth with chloroform on silica gel GF254 using ethyl acetate and hexane (1 : 1) as solvent showed three fractions. The major fraction (C3 ) was separated out and identified as 9-methyl-1, 4, 5, 8-tetra-azaphenanthrene. Acute toxicity test revealed the nontoxic nature upto a dose of 2000 mg kg(-1) , b.wt., of mice. MTT assay showed the cytotoxic nature in HL60 cells having an IC50 of 2.47 mmol. So, this biopigment may have application in food, textile colorant and pharmaceutical industry. This study demonstrated the optimum production of a biopigment (9-methyl-1, 4, 5, 8-tetra-azaphenanthrene) by fermentation of a complex medium with Bacillus cereus M(1) 16 (MTCC 5521) in submerged fermentation. This is the first investigation of toxicity and cytotoxicity activities of this biopigment. The study showed that the purified pigment had no toxicity to healthy albino mice but a high cytotoxicity activity in HL60 cancer cell line in vitro. The biopigment had further displayed dyeing capability to both solidified agar and cotton cloth. Therefore, it may represent a nontoxic and natural alternative to chemical dyes and pigments. © 2013 The Society for Applied Microbiology.

  12. Release of fission products and post-pile creep behaviour of irradiated fuel rods stored under dry conditions

    International Nuclear Information System (INIS)

    Kaspar, G.; Peehs, M.; Bokelmann, R.; Jorde, D.; Schoenfeld, H.; Haas, W.; Bleier, A.; Rutsch, F.

    1985-06-01

    The release of moisture and fission products (Kr-85, H-3 and I-129) under dry storage conditions has been examined on six fuel rods which have become defective in the reactor. During the examinations, inert conditions prevailed and limited air inlet was allowed temporarily. The storage temperature was 400 0 C. The residual moisture content of the fuel rods was approx. 5 g. At the beginning of the test, the total moisture content and 0,05% (max.) of the fission gas inventory were released. Under inert conditions, fission gas was not released during a prolonged period of time. Under oxidizing conditions, however, fission gas was released in the course of UO 2 oxidation. Post-pile creep of Zircaloy cladding tubes was measured at temperatures between 350 and 395 0 C and interval gauge pressures between 69 and 110 bar. The creep curves indicate that the irradiated cladding tube specimens still bear internal residual stresses which contribute through their relaxation to the post-pile creep. (orig.) [de

  13. Light intensity and production parameters of phytocenoses cultivated on soil-like substrate under controled environment conditions

    Science.gov (United States)

    Tikhomirov, A.; Ushakova, S.; Gribovskaya, I.; Tirranen, L.; Manukovsky, N.; Zolotukhin, I.

    To investigate feasibility of enhancing closedness in a new generation of biological life support systems (LSS) to involve the inedible phytomass into intrasystem mass exchange the vermicomposting method we have chosen made possible to produce soil-like substrate (SLS) suitable for growing plants. However, to use the SLS in life support systems call for investigation of its physical, chemical and other parameters. Of special importance among them is the capacity of SLS to provide the LSS photosynthesizing component with required mineral elements in selected cultivation conditions. In this connection the aim of this work was to study opportunities of enhancing pr4oduction activity of wheat and radish cenoses by varying the intensity of photosynthetically active radiation (PAR) without decreasing the harvest index. Increase of light intensity to 250 W/m 2 PAR decreased the intensity of visible photosynthesis of wheat cenosi and slightly increased visible photosynthesis of radish cenosis as compared to 200 W/m 2 PAR. The maximum productivity of wheat cenosis both total and seeds corresponded to the irradiance of 200 W/m 2 PAR. The light intensity of 250 W/m2 PAR decreased productivity of wheat plants and had no significant effect of the productivity of radish cenosis as compared to 200 W/m 2 PAR. Qualitative and quantitative composition of microflora of the watering solution and SLS was determined by the condition of plants, development phase and PAR intensity. By the end of wheat vegetation under 250 W/m 2 there were an order more bacteria of the colon rod group and phytopathogenic bacteria in the watering solution and SLS than under other illumination conditions. Investigation of the mineral composition of SLS and the watering solution demonstrated that one of the reasons of inadequate response of the cenosis under study to elevated PAR intensity may be deficiency of accessible forms of some mineral elements, e.g. nitrogen. The above said materials evidence that

  14. Production of gaseous and liquid chemicals by aqueous phase reforming of crude glycerol: Influence of operating conditions on the process

    International Nuclear Information System (INIS)

    Remón, J.; Giménez, J.R.; Valiente, A.; García, L.; Arauzo, J.

    2016-01-01

    Highlights: • Aqueous phase reforming: a tailor-made process for the valorisation of crude glycerol. • In-depth understanding of the effect of the operating conditions on the process. • Process optimisation for the selective production of valuable gas and liquid products. • Low pressure and high temperature and spatial time favour gas production. • High pressure and medium temperature maximise the production of valuable liquids. - Abstract: The present work studies the influence of the temperature (200–240 °C), pressure (38–50 bar), glycerol concentration (10–50 wt.%) and mass of catalyst/ glycerol mass flow rate ratio (W/m_g_l_y_c_e_r_o_l = 10–40 g catalyst min/g glycerol) during the aqueous phase reforming (APR) of a glycerol solution obtained from the production of biodiesel. The operating conditions exerted a statistically significant influence on the reforming results. Specifically, the global glycerol conversion and the carbon converted into gas and liquid products varied as follows: 4–100%, 1–80% and 16–93%, respectively. The gas phase was made up of H_2 (8–55 vol.%), CO_2 (34–66 vol.%), CO (0–4 vol.%) and CH_4 (6–45 vol.%). The liquid phase consisted of a mixture of alcohols (monohydric: methanol and ethanol; and polyhydric: 1,2-propanediol, 1,2-ethanediol, 2,3-butanediol), aldehydes (acetaldehyde), ketones (C3-ketones: acetone and 2-propanone-1-hydroxy; C4-ketones: 2-butanone-3-hydroxy and 2-butanone-1-hydroxy; and cyclic ketones), carboxylic acids (acetic and propionic acids) and esters (1,2,3-propanetriol-monoacetate), together with unreacted glycerol and water. The relative amount (free of water and un-reacted glycerol) of these compounds in the liquid phase was as follows: monohydric alcohols: 4–47%, polyhydric-alcohols: 14–68%, aldehydes: 0–5%, C3-ketones: 2–33%, C4-ketones: 0–10%, ciclo-ketones: 0–6%, carboxylic acids: 2–43%, and esters: 0–46%. This process turned out to be highly customisable for the

  15. Effect of Culture Conditions and Gamma Rays on Chitosan Production from Shrimp Shells by Certain Isolated Fungi

    International Nuclear Information System (INIS)

    Abd EI-Aziz, A.B.; Swialam, H.M.; Abd EI -Aziz, A.H.

    2009-01-01

    The obtained chitosan from shrimp shells waste used in the present work is compared with a standard chitosan. Six strains of the isolated fungi had the ability to attack the chitin namely: Candida tropicalis, Zygosaccharomyces rouxii, Candida guilliermondii, Trichoderma viride, Penicillium chrysogenum and Aspergillus niger. Z. rouxii and A. niger were the most active strains for decomposing chitin. The best culture conditions for chitosan production by the selected strains differed from one to another. Highest yield of chitosan was obtained after 96 h of incubation by A. niger (40.00 mg/g) in the basal medium followed by P. chrysogenum (38.20 mg/g). Optimum ph for chitosan production by C. tropicalis, Z. rouxii and T. viride was found to be 5.5, while ph 6.0 was the best for A. niger and C. guilliermondii. Meanwhile ph 5.0 was preferable for P. chrysogenllm. Regarding the carbon source, fructose as a sole carbon source in the medium was the best one for A. niger (92.58 mg/g) and T. viride (85.78 mg/g). C. tropicalis and Z. rouxii showed the highest chitosan production in the presence of sucrose (66.00 and 60.00 mg/g), whereas xylose was the best carbon for P. chrysogenum (62.50 mg/g). The selected strains were also differing in their nitrogen source requiring for production of chitosan. The present work confirmed that chitosan production by microorganisms is strongly dependent on the ph of the culture medium. The present data show that exposing the selected fungal strains to very low dose levels of gamma ray enhanced their productivity of chitosan and dry weight. The best environmental conditions of temperature degree, ph value and colloidal chitin concentration on chitinase activity produced by Z. rouxii were 30 degree C, 5.5 and 2% respectively, while they were 30 degree C, 6.0 and 1.5% for chitinase produced by A. niger in the same manner respectively

  16. Norepinephrine stimulates progesterone production in highly estrogenic bovine granulosa cells cultured under serum-free, chemically defined conditions.

    Science.gov (United States)

    Piccinato, Carla A; Montrezor, Luis H; Collares, Cristhianna A V; Vireque, Alessandra A; Rosa e Silva, Alzira A M

    2012-11-22

    Since noradrenergic innervation was described in the ovarian follicle, the actions of the intraovarian catecholaminergic system have been the focus of a variety of studies. We aimed to determine the gonadotropin-independent effects of the catecholamine norepinephrine (NE) in the steroid hormone profile of a serum-free granulosa cell (GC) culture system in the context of follicular development and dominance. Primary bovine GCs were cultivated in a serum-free, chemically defined culture system supplemented with 0.1% polyvinyl alcohol. The culture features were assessed by hormone measurements and ultrastructural characteristics of GCs. GCs produced increasing amounts of estradiol and pregnenolone for 144h and maintained ultrastructural features of healthy steroidogenic cells. Progesterone production was also detected, although it significantly increased only after 96h of culture. There was a highly significant positive correlation between estradiol and pregnenolone production in high E2-producing cultures. The effects of NE were further evaluated in a dose-response study. The highest tested concentration of NE (10 (-7) M) resulted in a significant increase in progesterone production, but not in estradiol or pregnenolone production. The specificity of NE effects on progesterone production was further investigated by incubating GCs with propranolol (10 (-8) M), a non-selective beta-adrenergic antagonist. The present culture system represents a robust model to study the impact of intrafollicular factors, such as catecholamines, in ovarian steroidogenesis and follicular development. The results of noradrenergic effects in the steroidogenesis of GC have implications on physiological follicular fate and on certain pathological ovarian conditions such as cyst formation and anovulation.

  17. Effect of body condition score at mating on the reproductive performance of Kivircik sheep under an extensive production system.

    Science.gov (United States)

    Yilmaz, Murat; Altin, Tufan; Karaca, Orhan; Cemal, Ibrahim; Bardakcioglu, Husnu Erbay; Yilmaz, Onur; Taskin, Turgay

    2011-12-01

    The aim of this study was to determine the effects of the body condition score (BCS) of ewes before and during the mating season on their reproductive performance under an extensive production system. In this study, 240 Kivircik sheep from the flocks of two breeders were used for over a 2-year period. The flocks were fed under extensive conditions on natural pastures throughout the year. The ewes were synchronized in two different periods, one of which was 45 days before the other. Before the introduction of rams to the ewes, the ewes were weighed after measuring their BCS. Detailed records were kept for every flock in each year. We found significant effects of BCS on pregnancy rate, lambing rate (P < 0.05) and fecundity (P < 0.05). The BCS for the highest pregnancy, lambing rate, and fecundity was determined between 2.01 and 3.00, while the lowest rates for these traits were ≤ 1.50. The highest rates of the pregnancy rate, lambing rate, and fecundity and gestation productivity were 75.9%, 70.9%, 1.11 and 3.34 kg, respectively.

  18. Chitosan as a novel clarifying agent on clear apple juice production: Optimization of process conditions and changes on quality characteristics.

    Science.gov (United States)

    Taştan, Özge; Baysal, Taner

    2017-12-15

    In this study, the use of chitosan as a clarification agent in the production of clear apple juice (AJ) was investigated, the optimum conditions were determined using RSM, and the effects on the quality of AJ were analyzed during storage at 4°C and 20°C for 4months. Optimum conditions for clarifying the AJ were chitosan concentration of 191.6mg/100ml juice, the process temperature of 20°C and process time of 30min. Changes in quality characteristics such as turbidity, pH, acidity, color, total monomeric anthocyanin, total phenolic, and protein contents were evaluated and compared to traditional clarification. Alicyclobacillus acidoterrestris causes significant safety issues in fruit juice industry; therefore also the antimicrobial effect of chitosan on A. acidoterrestris during the clarification process was examined. The results showed that clear AJ production can be achieved by the clarification step with direct chitosan addition, without the need for enzymatic treatment. However, the microbial reduction of A. acidoterrestris during the clarification process was not found to be significant. Copyright © 2017 Elsevier Ltd. All rights reserved.

  19. Scaling up for the industrial production of rifamycin B; optimization of the process conditions in bench-scale fermentor

    Directory of Open Access Journals (Sweden)

    Hewaida F. El-Sedawy

    2013-06-01

    Full Text Available Optimization of fermentation process conditions using a gene amplified variant of Amycolatopsis mediterranei (NCH was carried out. The use of aeration level 1.5 vvm increased the yield by 16.6% (from 13.81 to 16.1 g/l upon controlling the temperature at 28 °C. Adjustment of the aeration level at 1.5 vvm for 3 days then controlling the dissolved oxygen (DO at 30% saturation further increased the yield to 17.8 g/l. The optimum pH was 6.5 for 3 days then 7 thereafter when a production yield of 16.1 g/l was recorded using an aeration rate of 1.5 vvm. Controlling the pH at constant value (6.5 or 7 all through the fermentation process decreased the yield by 5–21%. Controlling the temperature at 30 °C for 3 days then 28 °C thereafter slightly increased the yield by 5% upon using an aeration rate of 1 vvm while it decreased upon using an aeration rate of 1.5 vvm. Integration of the most optimum conditions increased the production yield by 22% from 13.81 to 17.8 g/l.

  20. Health-related productivity losses increase when the health condition is co-morbid with psychological distress: findings from a large cross-sectional sample of working Australians

    Directory of Open Access Journals (Sweden)

    Vecchio Nerina

    2011-05-01

    Full Text Available Abstract Background The health condition of workers is known to impact on productivity outcomes. The relationship between health and productivity is of increasing interest amid the need to increase productivity to meet global financial challenges. Prevalence of psychological distress is also of growing concern in Australia with a two-fold increase in the prevalence of psychological distress in Australia from 1997-2005. Methods We used the cross-sectional data set from the Australian Work Outcomes Research Cost-benefit (WORC study to explore the impacts of health conditions with and without co-morbid psychological distress, compared to those with neither condition, in a sample of approximately 78,000 working Australians. The World Health Organisation Health and Performance Questionnaire was used which provided data on demographic characteristics, health condition and working conditions. Data were analysed using negative binomial logistic regression and multinomial logistic regression models for absenteeism and presenteeism respectively. Results For both absenteeism and presenteeism productivity measures there was a greater risk of productivity loss associated when health conditions were co-morbid with psychological distress. For some conditions this risk was much greater for those with co-morbid psychological distress compared to those without. Conclusions Co-morbid psychological distress demonstrates an increased risk of productivity loss for a range of health conditions. These findings highlight the need for further research to determine whether co-morbid psychological distress potentially exacerbates lost productivity.

  1. Health-related productivity losses increase when the health condition is co-morbid with psychological distress: findings from a large cross-sectional sample of working Australians.

    Science.gov (United States)

    Holden, Libby; Scuffham, Paul A; Hilton, Michael F; Ware, Robert S; Vecchio, Nerina; Whiteford, Harvey A

    2011-05-31

    The health condition of workers is known to impact on productivity outcomes. The relationship between health and productivity is of increasing interest amid the need to increase productivity to meet global financial challenges. Prevalence of psychological distress is also of growing concern in Australia with a two-fold increase in the prevalence of psychological distress in Australia from 1997-2005. We used the cross-sectional data set from the Australian Work Outcomes Research Cost-benefit (WORC) study to explore the impacts of health conditions with and without co-morbid psychological distress, compared to those with neither condition, in a sample of approximately 78,000 working Australians. The World Health Organisation Health and Performance Questionnaire was used which provided data on demographic characteristics, health condition and working conditions. Data were analysed using negative binomial logistic regression and multinomial logistic regression models for absenteeism and presenteeism respectively. For both absenteeism and presenteeism productivity measures there was a greater risk of productivity loss associated when health conditions were co-morbid with psychological distress. For some conditions this risk was much greater for those with co-morbid psychological distress compared to those without. Co-morbid psychological distress demonstrates an increased risk of productivity loss for a range of health conditions. These findings highlight the need for further research to determine whether co-morbid psychological distress potentially exacerbates lost productivity.

  2. Population ecology of the mallard: II. Breeding habitat conditions, size of the breeding populations, and production indices

    Science.gov (United States)

    Pospahala, Richard S.; Anderson, David R.; Henny, Charles J.

    1974-01-01

    This report, the second in a series on a comprehensive analysis of mallard population data, provides information on mallard breeding habitat, the size and distribution of breeding populations, and indices to production. The information in this report is primarily the result of large-scale aerial surveys conducted during May and July, 1955-73. The history of the conflict in resource utilization between agriculturalists and wildlife conservation interests in the primary waterfowl breeding grounds is reviewed. The numbers of ponds present during the breeding season and the midsummer period and the effects of precipitation and temperature on the number of ponds present are analyzed in detail. No significant cycles in precipitation were detected and it appears that precipitation is primarily influenced by substantial seasonal and random components. Annual estimates (1955-73) of the number of mallards in surveyed and unsurveyed breeding areas provided estimates of the size and geographic distribution of breeding mallards in North America. The estimated size of the mallard breeding population in North America has ranged from a high of 14.4 million in 1958 to a low of 7.1 million in 1965. Generally, the mallard breeding population began to decline after the 1958 peak until 1962, and remained below 10 million birds until 1970. The decline and subsequent low level of the mallard population between 1959 and 1969 .generally coincided with a period of poor habitat conditions on the major breeding grounds. The density of mallards was highest in the Prairie-Parkland Area with an average of nearly 19.2 birds per square mile. The proportion of the continental mallard breeding population in the Prairie-Parkland Area ranged from 30% in 1962 to a high of 600/0 in 1956. The geographic distribution of breeding mallards throughout North America was significantly related to the number of May ponds in the Prairie-Parkland Area. Estimates of midsummer habitat conditions and indices to

  3. Evaluation of the activated charcoals and adsorption conditions used in the treatment of sugarcane bagasse hydrolysate for xylitol production

    Directory of Open Access Journals (Sweden)

    J. M. Marton

    2006-03-01

    Full Text Available Xylitol has sweetening, anticariogenic and clinical properties that have attracted the attention of the food and pharmaceutical industries. The conversion of sugars from lignocellulosic biomass into xylitol by D-xylose-fermenting yeast represents an alternative to the chemical process for producing this polyol. A good source of D-xylose is sugarcane bagasse, which can be hydrolyzed with dilute acid. However, acetic acid, which is toxic to the yeast, also appears in the hydrolysate, inhibiting microbe metabolism. Xylitol production depends on the initial D-xylose concentration, which can be increased by concentrating the hydrolysate by vacuum evaporation. However, with this procedure the amount of acetic acid is also increased, aggravating the problem of cell inhibition. Hydrolysate treatment with powdered activated charcoal is used to remove or decrease the concentration of this inhibitor, improving xylitol productivity as a consequence. Our work was an attempt to improve the fermentation of Candida guilliermondii yeast in sugarcane bagasse hydrolysate by treating the medium with seven types of commercial powdered activated charcoals (Synth, Carbon Delta A, Carbon Delta G, Carbon 117, Carbon 118L, Carbon 147 and Carvorite, each with its own unique physicochemical properties. Various adsorption conditions were established for the variables temperature, contact time, shaking, pH and charcoal concentration. The experiments were based on multivariate statistical concepts, with the application of fractional factorial design techniques to identify the variables that are important in the process. Subsequently, the levels of these variables were quantified by overlaying the level curves, which permitted the establishment of the best adsorption conditions for attaining high levels of xylitol volumetric productivity and D-xylose-to-xylitol conversion. This procedure consisted in increasing the original pH of the hydrolysate to 7.0 with CaO and reducing it

  4. Fuel behaviour and fission product release under realistic hydrogen conditions comparisons between HEVA 06 test results and Vulcain computations

    International Nuclear Information System (INIS)

    Dumas, J.M.; Lhiaubet, G.

    1989-07-01

    The HEVA 06 test was designed to simulate the conditions existing at the time when fission products are released from irradiated fuel under hydrogen conditions occurring in a PWR core at low pressure. The test conditions were defined from results provided by the core degradation module of the ESCADRE system (1): VULCAIN. This computer code has been recently used to analyse the early core degradation of a 900 MWe PWR in the AF accident sequence (as defined in WASH - 1400, USNRC - 1975). In this scenario, the core would begin to uncover about one day after scram with the system pressure at about 0.4 MPa. The fission product release starts 70 minutes after core dewatering. The F.P. are transferred to the core outlet in an increasingly hydrogen-rich steam atmosphere. The carrier gas is nearly pure hydrogen in the time period 100 - 130 minutes after core uncovering. A large release of F.P. is predicted in the upper part of the core when the steam starvation occurs. At that time, two thirds of the cladding have been oxidised on an average. Before each HEVA test a fuel sample with a burn-up of 36 GWd/tU is reirradiated in order to observe the release of short-lived fission products. A pre-oxidation was primarely conducted in the HEVA 06 test at a temperature of 1300 0 C and controlled to reach a 2/3 cladding oxidation state. Then the steam was progressively replaced by hydrogen and a heat-up rate of 1.5 0 C/s was induced to reach a temperature of 2100 0 C. The fuel was maintained at this temperature for half an hour in hydrogen. The volatile F.P. release kinetics were observed by on-line gamma spectrometry. Pre test calculations of F.P. release kinetics performed with the EMIS module based on the CORSOR models (3) are compared with the test results. Measured releases of cesium and iodine are really lower than those predicted. Axial and radial F.P. distributions in the fuel pellets are available from gamma tomography measurements performed after the test. Tellurium seems

  5. Kinetics of corrosion products release from nickel-base alloys corroding in primary water conditions. A new modeling of release

    International Nuclear Information System (INIS)

    Carrette, F.; Guinard, L.; Pieraggi, B.

    2002-01-01

    The radioactivity in the primary circuit arises mainly from the activation of corrosion products in the core of pressurised water reactors; corrosion products dissolve from the oxide scales developed on steam generator tubes of alloy 690. The controlling and modelling of this process require a detailed knowledge of the microstructure and chemical composition of oxide scales as well as the kinetics of their corrosion and dissolution. Alloy 690 was studied as tubes and sheets, with three various surface states (as-received, cold-worked, electropolished). Corrosion tests were performed at 325 C and 155 bar in primary water conditions (B/Li - 1000/2 ppm, [H 2 ] 30 cm 3 .kg -1 TPN, [O 2 ] < 5 ppb); test durations ranged between 24 and 2160 hours. Corrosion tests in the TITANE loop provided mainly corrosion and oxidation kinetics, and tests in the BOREAL loop yielded release kinetics. This study revealed asymptotic type kinetics. Characterisation of the oxide scales grown in representative conditions of the primary circuit was performed by several techniques (SEM, TEM, SIMS, XPS, GIXRD). These analyses revealed the essential role of the fine grained cold-worked scale present on as-received and cold-worked materials. This scale controls the corrosion and release phenomena. The kinetic study and the characterisation of the oxide scales contributed to the modelling of the corrosion/release process. A growth/dissolution model was proposed for corrosion product scales grown in non-saturated dynamic fluid. This model provided the temporal evolution of oxide scales and release kinetics for different species (Fe, Ni, Cr). The model was validated for several surface states and several alloys. (authors)

  6. Technology development of protein rich concentrates for nutrition in extreme conditions using soybean and meat by-products.

    Science.gov (United States)

    Kalenik, Tatiana K; Costa, Rui; Motkina, Elena V; Kosenko, Tamara A; Skripko, Olga V; Kadnikova, Irina A

    2017-01-01

    There is a need to develop new foods for participants of expeditions in extreme conditions, which must be self-sufficient. These foods should be light to carry, with a long shelf life, tasty and with  high nutrient density. Currently, protein sources are limited mainly to dried and canned meat. In this work, a protein-rich dried concentrate suitable for extreme expeditions was developed using soya, tomato, milk whey and meat by-products. Protein concentrates were developed using minced beef liver and heart, dehydrated and mixed with a soya protein-lycopene coagulate (SPLC) obtained from a solution prepared with germi- nated soybeans and mixed with tomato paste in milk whey, and finally dried. The technological parameters of pressing SPLC and of drying the protein concentrate were optimized using response surface methodology. The optimized technological parameters to prepare the protein concentrates were obtained, with 70:30 being the ideal ratio of minced meat to SPLC. The developed protein concentrates are characterized by a high calorific value of 376 kcal/100 g of dry product, with a water content of 98 g·kg-1, and 641-644 g·kg-1 of proteins. The essential amino acid indices are 100, with minimum essential amino acid content constitut- ing 100-128% of the FAO standard, depending on the raw meat used. These concentrates are also rich in micronutrients such as β-carotene and vitamin C. Analysis of the nutrient content showed that these non-perishable concentrates present a high nutritional value and complement other widely available vegetable concentrates to prepare a two-course meal. The soups and porridges prepared with these concentrates can be classified as functional foods, and comply with army requirements applicable to food products for extreme conditions.

  7. Production of gasoline fraction from bio-oil under atmospheric conditions by an integrated catalytic transformation process

    International Nuclear Information System (INIS)

    Zhang, Zhaoxia; Bi, Peiyan; Jiang, Peiwen; Fan, Minghui; Deng, Shumei; Zhai, Qi; Li, Quanxin

    2015-01-01

    This work aimed to develop an integrated process for production of gasoline fraction bio-fuels from bio-oil under atmospheric conditions. This novel transformation process included the catalytic cracking of bio-oil to light olefins and the subsequent synthesis of liquid hydrocarbon bio-fuels from light olefins with two reactors in series. The yield of bio-fuel was up to 193.8 g/(kg bio-oil) along with a very low oxygen content, high RONs (research octane numbers), high LHVs (lower heating values) and low benzene content under the optimizing reaction conditions. Coke deposition seems to be the main cause of catalyst deactivation in view of the fact that the deactivated catalysts was almost recovered by on-line treating the used catalyst with oxygen. The integrated transformation potentially provides a useful way for the development of gasol