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  1. Reference Values for Spirometry Derived Using Lambda, Mu, Sigma (LMS) Method in Korean Adults: in Comparison with Previous References.

    Science.gov (United States)

    Jo, Bum Seak; Myong, Jun Pyo; Rhee, Chin Kook; Yoon, Hyoung Kyu; Koo, Jung Wan; Kim, Hyoung Ryoul

    2018-01-15

    The present study aimed to update the prediction equations for spirometry and their lower limits of normal (LLN) by using the lambda, mu, sigma (LMS) method and to compare the outcomes with the values of previous spirometric reference equations. Spirometric data of 10,249 healthy non-smokers (8,776 females) were extracted from the fourth and fifth versions of the Korea National Health and Nutrition Examination Survey (KNHANES IV, 2007-2009; V, 2010-2012). Reference equations were derived using the LMS method which allows modeling skewness (lambda [L]), mean (mu [M]), and coefficient of variation (sigma [S]). The outcome equations were compared with previous reference values. Prediction equations were presented in the following form: predicted value = e{a + b × ln(height) + c × ln(age) + M - spline}. The new predicted values for spirometry and their LLN derived using the LMS method were shown to more accurately reflect transitions in pulmonary function in young adults than previous prediction equations derived using conventional regression analysis in 2013. There were partial discrepancies between the new reference values and the reference values from the Global Lung Function Initiative in 2012. The results should be interpreted with caution for young adults and elderly males, particularly in terms of the LLN for forced expiratory volume in one second/forced vital capacity in elderly males. Serial spirometry follow-up, together with correlations with other clinical findings, should be emphasized in evaluating the pulmonary function of individuals. Future studies are needed to improve the accuracy of reference data and to develop continuous reference values for spirometry across all ages. © 2018 The Korean Academy of Medical Sciences.

  2. Reference values for spirometry, including vital capacity, in Japanese adults calculated with the LMS method and compared with previous values.

    Science.gov (United States)

    Kubota, Masaru; Kobayashi, Hirosuke; Quanjer, Philip H; Omori, Hisamitsu; Tatsumi, Koichiro; Kanazawa, Minoru

    2014-07-01

    Reference values for lung function tests should be periodically updated because of birth cohort effects and improved technology. This study updates the spirometric reference values, including vital capacity (VC), for Japanese adults and compares the new reference values with previous Japanese reference values. Spirometric data from healthy non-smokers (20,341 individuals aged 17-95 years, 67% females) were collected from 12 centers across Japan, and reference equations were derived using the LMS method. This method incorporates modeling skewness (lambda: L), mean (mu: M), and coefficient of variation (sigma: S), which are functions of sex, age, and height. In addition, the age-specific lower limits of normal (LLN) were calculated. Spirometric reference values for the 17-95-year age range and the age-dependent LLN for Japanese adults were derived. The new reference values for FEV(1) in males are smaller, while those for VC and FVC in middle age and elderly males and those for FEV(1), VC, and FVC in females are larger than the previous values. The LLN of the FEV(1)/FVC for females is larger than previous values. The FVC is significantly smaller than the VC in the elderly. The new reference values faithfully reflect spirometric indices and provide an age-specific LLN for the 17-95-year age range, enabling improved diagnostic accuracy. Compared with previous prediction equations, they more accurately reflect the transition in pulmonary function during young adulthood. In elderly subjects, the FVC reference values are not interchangeable with the VC values. Copyright © 2014 The Japanese Respiratory Society. Published by Elsevier B.V. All rights reserved.

  3. Mentoring to develop research selfefficacy, with particular reference to previously disadvantaged individuals

    OpenAIRE

    S. Schulze

    2010-01-01

    The development of inexperienced researchers is crucial. In response to the lack of research self-efficacy of many previously disadvantaged individuals, the article examines how mentoring can enhance the research self-efficacy of mentees. The study is grounded in the self-efficacy theory (SET) – an aspect of the social cognitive theory (SCT). Insights were gained from an in-depth study of SCT, SET and mentoring, and from a completed mentoring project. This led to the formulation of three basi...

  4. Mentoring to develop research selfefficacy, with particular reference to previously disadvantaged individuals

    Directory of Open Access Journals (Sweden)

    S. Schulze

    2010-07-01

    Full Text Available The development of inexperienced researchers is crucial. In response to the lack of research self-efficacy of many previously disadvantaged individuals, the article examines how mentoring can enhance the research self-efficacy of mentees. The study is grounded in the self-efficacy theory (SET – an aspect of the social cognitive theory (SCT. Insights were gained from an in-depth study of SCT, SET and mentoring, and from a completed mentoring project. This led to the formulation of three basic principles. Firstly, institutions need to provide supportive environmental conditions that facilitate research selfefficacy. This implies a supportive and efficient collective system. The possible effects of performance ratings and reward systems at the institution also need to be considered. Secondly, mentoring needs to create opportunities for young researchers to experience successful learning as a result of appropriate action. To this end, mentees need to be involved in actual research projects in small groups. At the same time the mentor needs to facilitate skills development by coaching and encouragement. Thirdly, mentors need to encourage mentees to believe in their ability to successfully complete research projects. This implies encouraging positive emotional states, stimulating self-reflection and self-comparison with others in the group, giving positive evaluative feedback and being an intentional role model.

  5. Tails from previous exposures: a general problem in setting reference levels for the assessment of internal contamination

    International Nuclear Information System (INIS)

    Breuer, F.; Frittelli, L.

    1988-01-01

    Reference levels for retention and excretion are evaluated for routine and special monitoring following the intake of a fraction of ICRP annual limits (ALIs) or of a unit activity. Methodologies are also suggested for taking into account the contribution by previous intakes to excretion or retention

  6. Proteomics Analysis Reveals Previously Uncharacterized Virulence Factors in Vibrio proteolyticus.

    Science.gov (United States)

    Ray, Ann; Kinch, Lisa N; de Souza Santos, Marcela; Grishin, Nick V; Orth, Kim; Salomon, Dor

    2016-07-26

    Members of the genus Vibrio include many pathogens of humans and marine animals that share genetic information via horizontal gene transfer. Hence, the Vibrio pan-genome carries the potential to establish new pathogenic strains by sharing virulence determinants, many of which have yet to be characterized. Here, we investigated the virulence properties of Vibrio proteolyticus, a Gram-negative marine bacterium previously identified as part of the Vibrio consortium isolated from diseased corals. We found that V. proteolyticus causes actin cytoskeleton rearrangements followed by cell lysis in HeLa cells in a contact-independent manner. In search of the responsible virulence factor involved, we determined the V. proteolyticus secretome. This proteomics approach revealed various putative virulence factors, including active type VI secretion systems and effectors with virulence toxin domains; however, these type VI secretion systems were not responsible for the observed cytotoxic effects. Further examination of the V. proteolyticus secretome led us to hypothesize and subsequently demonstrate that a secreted hemolysin, belonging to a previously uncharacterized clan of the leukocidin superfamily, was the toxin responsible for the V. proteolyticus-mediated cytotoxicity in both HeLa cells and macrophages. Clearly, there remains an armory of yet-to-be-discovered virulence factors in the Vibrio pan-genome that will undoubtedly provide a wealth of knowledge on how a pathogen can manipulate host cells. The pan-genome of the genus Vibrio is a potential reservoir of unidentified toxins that can provide insight into how members of this genus have successfully risen as emerging pathogens worldwide. We focused on Vibrio proteolyticus, a marine bacterium that was previously implicated in virulence toward marine animals, and characterized its interaction with eukaryotic cells. We found that this bacterium causes actin cytoskeleton rearrangements and leads to cell death. Using a

  7. Proteomics Analysis Reveals Previously Uncharacterized Virulence Factors in Vibrio proteolyticus

    Directory of Open Access Journals (Sweden)

    Ann Ray

    2016-07-01

    Full Text Available Members of the genus Vibrio include many pathogens of humans and marine animals that share genetic information via horizontal gene transfer. Hence, the Vibrio pan-genome carries the potential to establish new pathogenic strains by sharing virulence determinants, many of which have yet to be characterized. Here, we investigated the virulence properties of Vibrio proteolyticus, a Gram-negative marine bacterium previously identified as part of the Vibrio consortium isolated from diseased corals. We found that V. proteolyticus causes actin cytoskeleton rearrangements followed by cell lysis in HeLa cells in a contact-independent manner. In search of the responsible virulence factor involved, we determined the V. proteolyticus secretome. This proteomics approach revealed various putative virulence factors, including active type VI secretion systems and effectors with virulence toxin domains; however, these type VI secretion systems were not responsible for the observed cytotoxic effects. Further examination of the V. proteolyticus secretome led us to hypothesize and subsequently demonstrate that a secreted hemolysin, belonging to a previously uncharacterized clan of the leukocidin superfamily, was the toxin responsible for the V. proteolyticus-mediated cytotoxicity in both HeLa cells and macrophages. Clearly, there remains an armory of yet-to-be-discovered virulence factors in the Vibrio pan-genome that will undoubtedly provide a wealth of knowledge on how a pathogen can manipulate host cells.

  8. Discovery of previously undetected intellectual disability by psychological assessment: a study of consecutively referred child and adolescent psychiatric inpatients.

    Science.gov (United States)

    Pogge, David L; Stokes, John; Buccolo, Martin L; Pappalardo, Stephen; Harvey, Philip D

    2014-07-01

    Intellectual disability is associated with an increased risk of behavioral disturbances and also complicates their treatment. Despite increases in the sophistication of medical detection of early risk for intellectual disability, there is remarkably little data about the detection of intellectual disability in cases referred for psychiatric treatment. In this study, we used a 10-year sample of 23,629 consecutive child and adolescent admissions (ages between 6 and 17) to inpatient psychiatric treatment. Eleven percent (n=2621) of these cases were referred for psychological assessment and were examined with a general measure of intellectual functioning (i.e., WISC-IV). Of these cases, 16% had Full Scale IQs below 70. Of the cases whose therapists then referred them for formal assessment of their adaptive functioning (i.e., ABAS-II) 81% were found to have composite scores below 70 as well. Only one of the cases whose Full Scale IQ was less than 70 had a referral diagnosis of intellectual disability. Cases with previously undetected intellectual disability were found to be significantly more likely to have a diagnosis of a psychotic disorder and less likely to have a diagnosis of mood disorder than cases with IQs over 70. Disruptive behavior disorder diagnoses did not differ as a function of intellectual performance. These data suggest a high rate of undetected intellectual disability in cases with a psychiatric condition serious enough to require hospitalization and this raises the possibility that many such cases may be misdiagnosed, the basis of their problems may be misconceptualized, and they may be receiving treatments that do not take into account their intellectual level. Copyright © 2014 Elsevier Ltd. All rights reserved.

  9. Sacrococcygeal pilonidal disease: analysis of previously proposed risk factors

    Directory of Open Access Journals (Sweden)

    Ali Harlak

    2010-01-01

    Full Text Available PURPOSE: Sacrococcygeal pilonidal disease is a source of one of the most common surgical problems among young adults. While male gender, obesity, occupations requiring sitting, deep natal clefts, excessive body hair, poor body hygiene and excessive sweating are described as the main risk factors for this disease, most of these need to be verified with a clinical trial. The present study aimed to evaluate the value and effect of these factors on pilonidal disease. METHOD: Previously proposed main risk factors were evaluated in a prospective case control study that included 587 patients with pilonidal disease and 2,780 healthy control patients. RESULTS: Stiffness of body hair, number of baths and time spent seated per day were the three most predictive risk factors. Adjusted odds ratios were 9.23, 6.33 and 4.03, respectively (p<0.001. With an adjusted odds ratio of 1.3 (p<.001, body mass index was another risk factor. Family history was not statistically different between the groups and there was no specific occupation associated with the disease. CONCLUSIONS: Hairy people who sit down for more than six hours a day and those who take a bath two or less times per week are at a 219-fold increased risk for sacrococcygeal pilonidal disease than those without these risk factors. For people with a great deal of hair, there is a greater need for them to clean their intergluteal sulcus. People who engage in work that requires sitting in a seat for long periods of time should choose more comfortable seats and should also try to stand whenever possible.

  10. Radionuclides in Bayer process residues: previous analysis for radiological protection

    International Nuclear Information System (INIS)

    Cuccia, Valeria; Rocha, Zildete; Oliveira, Arno H. de

    2011-01-01

    Natural occurring radionuclides are present in many natural resources. Human activities may enhance concentrations of radionuclides and/or enhance potential of exposure to naturally occurring radioactive material (NORM). The industrial residues containing radionuclides have been receiving a considerable global attention, because of the large amounts of NORM containing wastes and the potential long term risks of long-lived radionuclides. Included in this global concern, this work focuses on the characterization of radioactivity in the main residues of Bayer process for alumina production: red mud and sand samples. Usually, the residues of Bayer process are named red mud, in their totality. However, in the industry where the samples were collected, there is an additional residues separation: sand and red mud. The analytical techniques used were gamma spectrometry (HPGe detector) and neutron activation analysis. The concentrations of radionuclides are higher in the red mud than in the sand. These solid residues present activities concentrations enhanced, when compared to bauxite. Further uses for the residues as building material must be more evaluated from the radiological point of view, due to its potential of radiological exposure enhancement, specially caused by radon emission. (author)

  11. Radionuclides in Bayer process residues: previous analysis for radiological protection

    Energy Technology Data Exchange (ETDEWEB)

    Cuccia, Valeria; Rocha, Zildete, E-mail: vc@cdtn.b, E-mail: rochaz@cdtn.b [Centro de Desenvolvimento da Tecnologia Nuclear (CDTN/CNEN-MG), Belo Horizonte, MG (Brazil); Oliveira, Arno H. de, E-mail: heeren@nuclear.ufmg.b [Universidade Federal de Minas Gerais (DEN/UFMG), Belo Horizonte, MG (Brazil). Dept. de Engenharia Nuclear

    2011-07-01

    Natural occurring radionuclides are present in many natural resources. Human activities may enhance concentrations of radionuclides and/or enhance potential of exposure to naturally occurring radioactive material (NORM). The industrial residues containing radionuclides have been receiving a considerable global attention, because of the large amounts of NORM containing wastes and the potential long term risks of long-lived radionuclides. Included in this global concern, this work focuses on the characterization of radioactivity in the main residues of Bayer process for alumina production: red mud and sand samples. Usually, the residues of Bayer process are named red mud, in their totality. However, in the industry where the samples were collected, there is an additional residues separation: sand and red mud. The analytical techniques used were gamma spectrometry (HPGe detector) and neutron activation analysis. The concentrations of radionuclides are higher in the red mud than in the sand. These solid residues present activities concentrations enhanced, when compared to bauxite. Further uses for the residues as building material must be more evaluated from the radiological point of view, due to its potential of radiological exposure enhancement, specially caused by radon emission. (author)

  12. Analysis of SOFCs Using Reference Electrodes

    Energy Technology Data Exchange (ETDEWEB)

    Finklea, H.; Chen, X.; Gerdes, K.; Pakalapati, S.; Celik, I.

    2013-01-01

    Reference electrodes are frequently applied to isolate the performance of one electrode in a solid oxide fuel cell. However, reference electrode simulations raise doubt to veracity of data collected using reference electrodes. The simulations predict that the reported performance for the one electrode will frequently contain performance of both electrodes. Nonetheless, recent reports persistently treat data so collected as ideally isolated. This work confirms the predictions of the reference electrode simulations on two SOFC designs, and to provides a method of validating the data measured in the 3-electrode configuration. Validation is based on the assumption that a change in gas composition to one electrode does not affect the impedance of the other electrode at open circuit voltage. This assumption is supported by a full physics simulation of the SOFC. Three configurations of reference electrode and cell design are experimentally examined using various gas flows and two temperatures. Impedance data are subjected to deconvolution analysis and equivalent circuit fitting and approximate polarization resistances of the cathode and anode are determined. The results demonstrate that the utility of reference electrodes is limited and often wholly inappropriate. Reported impedances and single electrode polarization values must be scrutinized on this basis.

  13. Service for victims of crime VDS info and victims’ support: Analysis of the previous work

    Directory of Open Access Journals (Sweden)

    Ćopić Sanja M.

    2004-01-01

    Full Text Available The first victim support service in our country VDS info and victims’ support started with its work in April 2003 within the Victimology Society of Serbia. This service is aimed at victims of crime (women and men, primarily at victims of violent crime, but also of some forms of property crime (such as burglary. The aim of the Service is to offer victims of crime information on their rights and the ways of how to realize them, emotional support, as well as to refer them to other institutions/organizations depending on the certain victim’s needs. Coordinators and volunteers, who passed the appropriate training, are responsible for that. Bearing that in mind, this paper will give the brief glens on the Service itself, its organization and the way of work, followed by the analysis of the results of previous work.

  14. Analysis Of Navy Hornet Squadron Mishap Costs With Regard To Previously Flown Flight Hours

    Science.gov (United States)

    2017-06-01

    HORNET SQUADRON MISHAP COSTS WITH REGARD TO PREVIOUSLY FLOWN FLIGHT HOURS by Jason R. Baumann June 2017 Thesis Advisor: Robert Eger Co...TYPE AND DATES COVERED Master’s thesis 4. TITLE AND SUBTITLE ANALYSIS OF NAVY HORNET SQUADRON MISHAP COSTS WITH REGARD TO PREVIOUSLY FLOWN...flown flight hours with mishap costs . It uses a macro level approach by evaluating how a squadron’s previously flown flight hours affect mishap cost and

  15. Intelligence, previous convictions and interrogative suggestibility: a path analysis of alleged false-confession cases.

    Science.gov (United States)

    Sharrock, R; Gudjonsson, G H

    1993-05-01

    The main purpose of this study was to investigate the relationship between interrogative suggestibility and previous convictions among 108 defendants in criminal trials, using a path analysis technique. It was hypothesized that previous convictions, which may provide defendants with interrogative experiences, would correlate negatively with 'shift' as measured by the Gudjonsson Suggestibility Scale (Gudjonsson, 1984a), after intelligence and memory had been controlled for. The hypothesis was partially confirmed and the theoretical and practical implications of the findings are discussed.

  16. Certified reference materials for organic analysis

    International Nuclear Information System (INIS)

    QianChuanfan

    2002-01-01

    This presentation discusses the requirements for certified reference materials (CRMs) to be used in measurements of residues of pesticides in food and environmental samples. It deals with the types of CRMs, matrix selection, sample preparation and representativeness. It also discusses CRM validity period and gives some examples of CRM preparation

  17. Nordic reference study on uncertainty and sensitivity analysis

    International Nuclear Information System (INIS)

    Hirschberg, S.; Jacobsson, P.; Pulkkinen, U.; Porn, K.

    1989-01-01

    This paper provides a review of the first phase of Nordic reference study on uncertainty and sensitivity analysis. The main objective of this study is to use experiences form previous Nordic Benchmark Exercises and reference studies concerning critical modeling issues such as common cause failures and human interactions, and to demonstrate the impact of associated uncertainties on the uncertainty of the investigated accident sequence. This has been done independently by three working groups which used different approaches to modeling and to uncertainty analysis. The estimated uncertainty interval for the analyzed accident sequence is large. Also the discrepancies between the groups are substantial but can be explained. Sensitivity analyses which have been carried out concern e.g. use of different CCF-quantification models, alternative handling of CCF-data, time windows for operator actions and time dependences in phase mission operation, impact of state-of-knowledge dependences and ranking of dominating uncertainty contributors. Specific findings with respect to these issues are summarized in the paper

  18. The suitability of XRF analysis for compositional classification of archaeological ceramic fabric: A comparison with a previous NAA study

    Energy Technology Data Exchange (ETDEWEB)

    Padilla, R. [Centro de Aplicaciones Tecnologicas y Desarrollo Nuclear (CEADEN), Laboratorio de Analisis Quimico, Calle 30 no. 502, Playa, Ciudad Habana (Cuba)]. E-mail: roman.padilla@infomed.sld.cu; Espen, P. van [University of Antwerp (Belgium); Torres, P.P. Godo [Centro de Antropologia, Havana (Cuba)

    2006-02-03

    The main drawbacks of EDXRF techniques, restricting its more frequent use for the specific purpose of compositional analysis of archaeological ceramic fabric, have been the insufficient sensitivity to determine some important elements (like Cr, REE, among others), a somewhat worse precision and the inability to perform standard-less quantitative procedures in the absence of suitable certified reference materials (CRM) for ceramic fabric. This paper presents the advantages of combining two energy dispersive X-ray fluorescence methods for fast and non-destructive analysis of ceramic fabric with increased sensitivity. Selective polarized excitation using secondary targets (EDPXRF) and radioisotope excitation (R-XRF) using a {sup 241}Am source. The analytical performance of the methods was evaluated by analyzing several CRM of sediment type, and the fitness for the purpose of compositional classification was compared with that obtained by using Instrumental Neutron Activation Analysis in a previous study of Cuban aborigine pottery.

  19. Comparison of Updated Weight and Height Percentiles with Previous References in 6-17-Year-Old Children in Kayseri, Turkey.

    Science.gov (United States)

    Zararsız, Gökmen; Çiçek, Betül; Kondolot, Meda; Mazıcıoğlu, M Mümtaz; Öztürk, Ahmet; Kurtoğlu, Selim

    2017-03-01

    To compare updated weight and height percentiles of 6-17-year-old children from all socio-economic levels in Kayseri with previous local references and other national/international data. The second study "Determination of Anthropometric Measurements of Turkish Children and Adolescents study (DAMTCA II)" was conducted in Kayseri, between October 2007 and April 2008. Weight and height measurements from 4321 (1926 boys, 2395 girls) school children aged between 6 to 17 years were included in this cross-sectional study. Using these data, weight and height percentile curves were produced with generalized additive models for location, scale and shape (GAMLSS) and compared with the most recent references. Smoothed percentile curves including the 3 rd , 5 th , 10 th , 15 th , 25 th , 50 th , 75 th , 85 th , 90 th , 95 th , and 97 th percentiles were obtained for boys and girls. These results were compared with DAMTCA I study and with two national (İstanbul and Ankara) and international data from Asia and from Europe. This study provides updated weight and height references for Turkish school children aged between 6 and 17 years residing in Kayseri.

  20. Upon Further Review: V. An Examination of Previous Lightcurve Analysis from the Palmer Divide Observatory

    Science.gov (United States)

    Warner, Brian D.

    2011-01-01

    Updated results are given for nine asteroids previously reported from the Palmer Divide Observatory (PDO). The original images were re-measured to obtain new data sets using the latest version of MPO Canopus photometry software, analysis tools, and revised techniques for linking multiple observing runs covering several days to several weeks. Results that were previously not reported or were moderately different were found for 1659 Punkajarju, 1719 Jens, 1987 Kaplan, 2105 Gudy, 2961 Katsurahama, 3285 Ruth Wolfe, 3447 Burckhalter, 7816 Hanoi, and (34817) 2000 SE116. This is one in a series of papers that will examine results obtained during the initial years of the asteroid lightcurve program at PDO.

  1. Further analysis of previously implicated linkage regions for Alzheimer's disease in affected relative pairs

    Directory of Open Access Journals (Sweden)

    Lannfelt Lars

    2009-12-01

    Full Text Available Abstract Background Genome-wide linkage studies for Alzheimer's disease have implicated several chromosomal regions as potential loci for susceptibility genes. Methods In the present study, we have combined a selection of affected relative pairs (ARPs from the UK and the USA included in a previous linkage study by Myers et al. (Am J Med Genet, 2002, with ARPs from Sweden and Washington University. In this total sample collection of 397 ARPs, we have analyzed linkage to chromosomes 1, 9, 10, 12, 19 and 21, implicated in the previous scan. Results The analysis revealed that linkage to chromosome 19q13 close to the APOE locus increased considerably as compared to the earlier scan. However, linkage to chromosome 10q21, which provided the strongest linkage in the previous scan could not be detected. Conclusion The present investigation provides yet further evidence that 19q13 is the only chromosomal region consistently linked to Alzheimer's disease.

  2. Do emotional intelligence and previous caring experience influence student nurse performance? A comparative analysis.

    Science.gov (United States)

    Stenhouse, Rosie; Snowden, Austyn; Young, Jenny; Carver, Fiona; Carver, Hannah; Brown, Norrie

    2016-08-01

    Reports of poor nursing care have focused attention on values based selection of candidates onto nursing programmes. Values based selection lacks clarity and valid measures. Previous caring experience might lead to better care. Emotional intelligence (EI) might be associated with performance, is conceptualised and measurable. To examine the impact of 1) previous caring experience, 2) emotional intelligence 3) social connection scores on performance and retention in a cohort of first year nursing and midwifery students in Scotland. A longitudinal, quasi experimental design. Adult and mental health nursing, and midwifery programmes in a Scottish University. Adult, mental health and midwifery students (n=598) completed the Trait Emotional Intelligence Questionnaire-short form and Schutte's Emotional Intelligence Scale on entry to their programmes at a Scottish University, alongside demographic and previous caring experience data. Social connection was calculated from a subset of questions identified within the TEIQue-SF in a prior factor and Rasch analysis. Student performance was calculated as the mean mark across the year. Withdrawal data were gathered. 598 students completed baseline measures. 315 students declared previous caring experience, 277 not. An independent-samples t-test identified that those without previous caring experience scored higher on performance (57.33±11.38) than those with previous caring experience (54.87±11.19), a statistically significant difference of 2.47 (95% CI, 0.54 to 4.38), t(533)=2.52, p=.012. Emotional intelligence scores were not associated with performance. Social connection scores for those withdrawing (mean rank=249) and those remaining (mean rank=304.75) were statistically significantly different, U=15,300, z=-2.61, p$_amp_$lt;0.009. Previous caring experience led to worse performance in this cohort. Emotional intelligence was not a useful indicator of performance. Lower scores on the social connection factor were associated

  3. Analysis of Product Buying Decision on Lazada E-commerce based on Previous Buyers’ Comments

    OpenAIRE

    Neil Aldrin

    2017-01-01

    The aims of the present research are: 1) to know that product buying decision possibly occurs, 2) to know how product buying decision occurs on Lazada e-commerce’s customers, 3) how previous buyers’ comments can increase product buying decision on Lazada e-commerce. This research utilizes qualitative research method. Qualitative research is a research that investigates other researches and makes assumption or discussion result so that other analysis results can be made in order to widen idea ...

  4. Determination of ancient ceramics reference material by neutron activation analysis

    International Nuclear Information System (INIS)

    Li Huhou; Sun Jingxin; Wang Yuqi; Lu Liangcai

    1986-01-01

    Contents of trace elements in the reference material of ancient ceramics (KPS-1) were determined by means of activation analysis, using thermal neutron irradiation produced in nuclear reactor. KPS-1 favoured the analysis of ancient ceramics because it had not only many kinds of element but also appropriate contents of composition. The values presented here are reliable within the experimental precision, and have shown that the reference material had a good homogeneity. So KPS-1 can be used as a suitable reference material for the ancient ceramics analysis

  5. Budget impact analysis of empagliflozin in T2D patients with a previous cardiovascular disease

    Directory of Open Access Journals (Sweden)

    Sergio Iannazzo

    2017-06-01

    Full Text Available Budget impact analysis of empagliflozin in T2D patients with a previous cardiovascular diseaseIntroductionThe EMPA-REG OUTCOME study demonstrated that empagliflozin is effective in reducing cardiovascular morbidity and mortality in T2D patients with high risk linked to a previous cardiovascular condition. The objective of this study is the budget impact analysis of the use of empagliflozin in this population in Italy.MethodsThe analysis was conducted from the cost perspective of the Italian NHS with a 3-year time horizon. The target population, defined as T2D patients with a known cardiovascular disease, was estimated based on epidemiologic and market data. An uptake of empagliflozin growing from 10% to 30% was considered to add up or substitute combinations of metformin, insulin, glitazones and DPP-4. The uptake of 80-100% was considered to substitute sulfonylureas and repaglinide. Costs, including drugs, glycaemia monitoring, management of severe hypoglycaemia events, and cardiovascular and renal events, were estimated based on published literature and current tariffs.ResultsThe target population was estimated in 243,693 in the first year. The incremental cost for drugs (17.6 million Euro is entirely offset by the savings in glycaemia monitoring (8.1 million Euro, cardiovascular and renal events (8.8 million Euro and severe hypoglycaemic events (3.1 million Euro, with a net saving of 2.3 million Euro, approximately corresponding to -0.7% of the total expenditure. The cumulative budget impact in the 3 years was 4.9 million Euro (-0.5%.ConclusionsThe progressive adoption of empagliflozin to treat T2D patients with known cardiovascular disease in Italy, would provide significant clinical benefit without entailing additional expense for the Italian NHS.

  6. Analysis of E-mail Transactions in Virtual Reference Services

    Directory of Open Access Journals (Sweden)

    Astutik Nur Qomariyah

    2016-01-01

    Full Text Available Today, the use of traditional reference desk in the academic libraries has been rarely used, thus expanding or even move to a virtual reference service. A minimum level of virtual reference services are provided in the academic library is currently in general is the electronic mail (e-mail. One of the academic library specifically provide virtual reference services via e-mail is a Petra Christian University (PCU Library (ref-desk@petra.ac.id.. In such services librarians provide assistance to users in finding information and answer questions. This study aimed to analyze the transaction reference services virtually through e-mail at the PCU Library, with a view of the types of questions based on user background, the writing style of language communication interaction used based on user background, and cultural values are revealed behind the user in virtual reference services (e-mail. This study uses content analysis (content analysis of the transcript e-mail received librarians of reference services began March 10 until June 16, 2015. The results showed that the types of questions asked in reference service virtual (e-mail in the Library UK Petra include: specific search, access online resources, operation of online resources, policies and procedures for services, and library holdings with background the student (PCU and non-PCU, faculty, and librarians. Based on the background of users found that overall more types of questions asked in virtual reference services (e-mail is a problem of access to online resources, and generally submitted by the students. Then, the writing style of the user's language in interaction reference service virtual (e-mail tends to be formal, which includes the word greeting, the message will be delivered, and regards cover, either by the student (PCU and non-PCU, lecturer, or librarians. While cultural values that revealed the background behind the user in virtual reference services (e-mail is obedience, courtesy and

  7. Analysis of Product Buying Decision on Lazada E-commerce based on Previous Buyers’ Comments

    Directory of Open Access Journals (Sweden)

    Neil Aldrin

    2017-06-01

    Full Text Available The aims of the present research are: 1 to know that product buying decision possibly occurs, 2 to know how product buying decision occurs on Lazada e-commerce’s customers, 3 how previous buyers’ comments can increase product buying decision on Lazada e-commerce. This research utilizes qualitative research method. Qualitative research is a research that investigates other researches and makes assumption or discussion result so that other analysis results can be made in order to widen idea and opinion. Research result shows that product which has many ratings and reviews will trigger other buyers to purchase or get that product. The conclusion is that product buying decision may occur because there are some processes before making decision which are: looking for recognition and searching for problems, knowing the needs, collecting information, evaluating alternative, evaluating after buying. In those stages, buying decision on Lazada e-commerce is supported by price, promotion, service, and brand.

  8. Analysis of E-mail Transactions in Virtual Reference Services

    Directory of Open Access Journals (Sweden)

    Astutik Nur Qomariyah

    2018-01-01

    Full Text Available Today, the use of traditional reference desk in the academic libraries has been rarely used, thus expanding or even move to a virtual reference service. A minimum level of virtual reference services are provided in the academic library is currently in general is the electronic mail (e-mail. One of the academic library specifically provide virtual reference services via e-mail is a Petra Christian University (PCU Library (refdesk@petra.ac.id.. In such services librarians provide assistance to users in finding information and answer questions. This study aimed to analyze the transaction reference services virtually through e-mail at the PCU Library, with a view of the types of questions based on user background, the writing style of language communication interaction used based on user background, and cultural values are revealed behind the user in virtual reference services (e-mail. This study uses content analysis (content analysis of the transcript e-mail received librarians of reference services began March 10 until June 16, 2015. The results showed that the types of questions asked in reference service virtual (e-mail in the Library UK Petra include: specific search, access online resources, operation of online resources, policies and procedures for services, and library holdings with background the student (PCU and non-PCU, faculty, and librarians. Based on the background of users found that overall more types of questions asked in virtual reference services (e-mail is a problem of access to online resources, and generally submitted by the students. Then, the writing style of the user's language in interaction reference service virtual (e-mail tends to be formal, which includes the word greeting, the message will be delivered, and regards cover, either by the student (PCU and non-PCU, lecturer, or librarians. While cultural values that revealed the background behind the user in virtual reference services (e-mail is obedience, courtesy and

  9. Establishment of reference intervals and transfusion criterion for Sonoclot analysis.

    Science.gov (United States)

    Zhang, Zhen-Lu; Chen, You-Ping; Tao, Cui-Hua; Liu, Xiao-Hui; Li, Meng-Ya; Zhou, Xin

    2016-08-01

    Sonoclot analyzer has been widely used in many countries. But the reference intervals provided by the manufacturer were derived from only 45 participants, and there was no cut-off value for transfusion for Sonoclot analysis. This study aimed to establish reference intervals and transfusion criterion for Sonoclot analysis. Volunteers were recruited from healthy Chinese adults and patients undergoing cardiac surgery. Blood samples were withdrawn from forearm vein and measured for activated clotting time (ACT), clot rate (CR), platelet function (PF), activated partial thromboplastin time (APTT), fibrinogen concentration (FIB), and platelet count (PLT). The reference intervals were determined by the nonparametric method. Cut-off values were determined by the receiver operating characteristics curve. A total of 135 healthy volunteers and 281 patients were enrolled. The 95% reference intervals were 96-195 s, 22-51 signal U/min, >1.6 for ACT, CR, PF respectively. In the 281 patients, the results of APTT, FIB, PLT, ACT, CR, and PF ranged from 20.5-300.0 s, 0.28-4.11 g/L, (19.0-387.3)×109/L, 80-514 s, 2.9-74 signal U/min, and 0.1-5.1 respectively. The cut-off values for transfusion were >208, ≤14, and ≤1.3 for ACT, CR, PF respectively. The cut-off values of Sonoclot analysis were within the manufacturer's reference intervals, while they were outside the reference intervals established in this study. The results suggested that the manufacturer's reference intervals were not suitable for Chinese. The reference intervals and cut-off values established in this study will be helpful to Chinese patients.

  10. A new certified reference material for size analysis of nanoparticles

    International Nuclear Information System (INIS)

    Braun, Adelina; Kestens, Vikram; Franks, Katrin; Roebben, Gert; Lamberty, Andrée; Linsinger, Thomas P. J.

    2012-01-01

    A certified reference material, ERM-FD100, for quality assurance and validation of various nanoparticle sizing methods, was developed by the Institute for Reference Materials and Measurements. The material was prepared from an industrially sourced colloidal silica containing nanoparticles with a nominal equivalent spherical diameter of 20 nm. The homogeneity and stability of the candidate reference material was assessed by means of dynamic light scattering and centrifugal liquid sedimentation. Certification of the candidate reference material was based on a global interlaboratory comparison in which 34 laboratories participated with various analytical methods (DLS, CLS, EM, SAXS, ELS). After scrutinising the interlaboratory comparison data, 4 different certified particle size values, specific for the corresponding analytical method, could be assigned. The good comparability of results allowed the certification of the colloidal silica material for nanoparticle size analysis.

  11. Certification of biological candidates reference materials by neutron activation analysis

    Science.gov (United States)

    Kabanov, Denis V.; Nesterova, Yulia V.; Merkulov, Viktor G.

    2018-03-01

    The paper gives the results of interlaboratory certification of new biological candidate reference materials by neutron activation analysis recommended by the Institute of Nuclear Chemistry and Technology (Warsaw, Poland). The correctness and accuracy of the applied method was statistically estimated for the determination of trace elements in candidate reference materials. The procedure of irradiation in the reactor thermal fuel assembly without formation of fast neutrons was carried out. It excluded formation of interfering isotopes leading to false results. The concentration of more than 20 elements (e.g., Ba, Br, Ca, Co, Ce, Cr, Cs, Eu, Fe, Hf, La, Lu, Rb, Sb, Sc, Ta, Th, Tb, Yb, U, Zn) in candidate references of tobacco leaves and bottom sediment compared to certified reference materials were determined. It was shown that the average error of the applied method did not exceed 10%.

  12. Validation of a reference gene for transcript analysis in cassava ...

    African Journals Online (AJOL)

    RT-qPCR) is a well-established method for the precise quantification of gene expression. For accurate relative real-time RT-qPCR analysis, validation of the expression of an appropriate reference gene is required. In this study, the expression of ...

  13. On criteria for examining analysis quality with standard reference material

    International Nuclear Information System (INIS)

    Yang Huating

    1997-01-01

    The advantages and disadvantages and applicability of some criteria for examining analysis quality with standard reference material are discussed. The combination of the uncertainties of the instrument examined and the reference material should be determined on the basis of specific situations. Without the data of the instrument's uncertainty, it would be applicable to substitute the standard deviation multiplied by certain times for the uncertainty. The result of the examining should not result in more error reported in routine measurements than it really is. Over strict examining should also be avoided

  14. Error analysis of aspheric surface with reference datum.

    Science.gov (United States)

    Peng, Yanglin; Dai, Yifan; Chen, Shanyong; Song, Ci; Shi, Feng

    2015-07-20

    Severe requirements of location tolerance provide new challenges for optical component measurement, evaluation, and manufacture. Form error, location error, and the relationship between form error and location error need to be analyzed together during error analysis of aspheric surface with reference datum. Based on the least-squares optimization method, we develop a least-squares local optimization method to evaluate form error of aspheric surface with reference datum, and then calculate the location error. According to the error analysis of a machined aspheric surface, the relationship between form error and location error is revealed, and the influence on the machining process is stated. In different radius and aperture of aspheric surface, the change laws are simulated by superimposing normally distributed random noise on an ideal surface. It establishes linkages between machining and error analysis, and provides an effective guideline for error correcting.

  15. Analysis of previous perceptual and motor experience in breaststroke kick learning

    Directory of Open Access Journals (Sweden)

    Ried Bettina

    2015-12-01

    Full Text Available One of the variables that influence motor learning is the learner’s previous experience, which may provide perceptual and motor elements to be transferred to a novel motor skill. For swimming skills, several motor experiences may prove effective. Purpose. The aim was to analyse the influence of previous experience in playing in water, swimming lessons, and music or dance lessons on learning the breaststroke kick. Methods. The study involved 39 Physical Education students possessing basic swimming skills, but not the breaststroke, who performed 400 acquisition trials followed by 50 retention and 50 transfer trials, during which stroke index as well as rhythmic and spatial configuration indices were mapped, and answered a yes/no questionnaire regarding previous experience. Data were analysed by ANOVA (p = 0.05 and the effect size (Cohen’s d ≥0.8 indicating large effect size. Results. The whole sample improved their stroke index and spatial configuration index, but not their rhythmic configuration index. Although differences between groups were not significant, two types of experience showed large practical effects on learning: childhood water playing experience only showed major practically relevant positive effects, and no experience in any of the three fields hampered the learning process. Conclusions. The results point towards diverse impact of previous experience regarding rhythmic activities, swimming lessons, and especially with playing in water during childhood, on learning the breaststroke kick.

  16. Differential DNA Methylation Analysis without a Reference Genome

    Directory of Open Access Journals (Sweden)

    Johanna Klughammer

    2015-12-01

    Full Text Available Genome-wide DNA methylation mapping uncovers epigenetic changes associated with animal development, environmental adaptation, and species evolution. To address the lack of high-throughput methods for DNA methylation analysis in non-model organisms, we developed an integrated approach for studying DNA methylation differences independent of a reference genome. Experimentally, our method relies on an optimized 96-well protocol for reduced representation bisulfite sequencing (RRBS, which we have validated in nine species (human, mouse, rat, cow, dog, chicken, carp, sea bass, and zebrafish. Bioinformatically, we developed the RefFreeDMA software to deduce ad hoc genomes directly from RRBS reads and to pinpoint differentially methylated regions between samples or groups of individuals (http://RefFreeDMA.computational-epigenetics.org. The identified regions are interpreted using motif enrichment analysis and/or cross-mapping to annotated genomes. We validated our method by reference-free analysis of cell-type-specific DNA methylation in the blood of human, cow, and carp. In summary, we present a cost-effective method for epigenome analysis in ecology and evolution, which enables epigenome-wide association studies in natural populations and species without a reference genome.

  17. Retrospective analysis on malignant calcification previously misdiagnosed as benign on screening mammography

    Energy Technology Data Exchange (ETDEWEB)

    Ha, Su Min [Dept. of Radiology, Research Institute of Radiology, Chung Ang University Hospital, Seoul(Korea, Republic of); Cha, Joo Hee; Kim, Hak Hee; Shin, Hee Jung; Chae, Eun Young; Choi, Woo Jung [Dept. of Radiology, Research Institute of Radiology, Asan Medical Center, University of Ulsan College of Medicine, Seoul (Korea, Republic of)

    2017-04-15

    The purpose of our study was to investigate the morphology and distribution of calcifications initially interpreted as benign or probably benign, but proven to be malignant by subsequent stereotactic biopsy, and to identify the reason for misinterpretation or underestimation at the initial diagnosis. Out of 567 women who underwent stereotactic biopsy for calcifications at our hospital between January 2012 and December 2014, 167 women were diagnosed with malignancy. Forty-six of these 167 women had previous mammography assessed as benign or probably benign which was changed to suspicious malignancy on follow-up mammography. Of these 46 women, three women with biopsy-proven benign calcifications at the site of subsequent cancer were excluded, and 43 patients were finally included. The calcifications (morphology, distribution, extent, associated findings) in the previous and follow-up mammography examinations were analyzed according to the Breast Imaging Reporting and Data System (BI-RADS) lexicon and assessment category. We classified the patients into two groups: 1) group A patients who were still retrospectively re-categorized as less than or equal to BI-RADS 3 and 2) group B patients who were re-categorized as equal to or higher than BI-RADS 4a and whose results should have prompted previous diagnostic assessment. In the follow-up mammography examinations, change in calcification morphology (n = 27, 63%) was the most frequent cause of assessment change. The most frequent previous mammographic findings of malignant calcification were amorphous morphology (n = 26, 60%) and grouped distribution (n = 36, 84%). The most frequent calcification findings at reassessment were amorphous morphology (n = 4, 9%), fine pleomorphic calcification (n = 30, 70%), grouped distribution (n = 23, 53%), and segmental calcification (n = 12, 28%). There were 33 (77%) patients in group A, and 10 patients (23%) in group B. Amorphous morphology and grouped distribution were the most frequent

  18. Neutron activation analysis for certification of standard reference materials

    International Nuclear Information System (INIS)

    Capote Rodriguez, G.; Perez Zayas, G.; Hernandez Rivero, A.; Ribeiro Guevara, S.

    1996-01-01

    Neutron activation analysis is used extensively as one of the analytical techniques in the certification of standard reference materials. Characteristics of neutron activation analysis which make it valuable in this role are: accuracy multielemental capability to asses homogeneity, high sensitivity for many elements, and essentially non-destructive method. This paper report the concentrations of 30 elements (major, minor and trace elements) in four Cuban samples. The samples were irradiated in a thermal neutron flux of 10 12- 10 13 n.cm 2. s -1. The gamma ray spectra were measured by HPGe detectors and were analyzed using ACTAN program development in Center of Applied Studies for Nuclear Development

  19. Kidney transplantation after previous liver transplantation: analysis of the organ procurement transplant network database.

    Science.gov (United States)

    Gonwa, Thomas A; McBride, Maureen A; Mai, Martin L; Wadei, Hani M

    2011-07-15

    Patients after liver transplant have a high incidence of chronic kidney disease and end-stage renal disease (ESRD). We investigated kidney transplantation after liver transplantation using the Organ Procurement Transplant Network database. The Organ Procurement Transplant Network database was queried for patients who received kidney transplantation after previous liver transplantation. These patients were compared with patients who received primary kidney transplantation alone during the same time period. Between 1997 and 2008, 157,086 primary kidney transplants were performed. Of these, 680 deceased donor kidney transplants and 410 living donor kidney transplants were performed in previous recipients of liver transplants. The number of kidney after liver transplants performed each year has increased from 37 per year to 124 per year in 2008. The time from liver transplant to kidney transplant increased from 8.2 to 9.0 years for living donor transplants and from 5.4 to 9.6 years for deceased donor. The 1, 3, and 5 year actuarial graft survival in both living donor kidney after liver transplant and deceased donor kidney after liver transplant are less than the kidney transplant alone patients. However, the death-censored graft survivals are equal. The patient survival is also less but is similar to what would be expected in liver transplant recipients who did not have ESRD. In 2008, kidney after liver transplantation represented 0.9% of the total kidney alone transplants performed in the United States. Kidney transplantation is an appropriate therapy for selected patients who develop ESRD after liver transplantation.

  20. Rhabdomyosarcoma Arising in a Previously Irradiated Field: An Analysis of 43 Patients

    Energy Technology Data Exchange (ETDEWEB)

    Dang, Nguyen D. [Department of Radiation Oncology, Baylor College of Medicine, Houston, Texas (United States); Teh, Bin S. [Department of Radiation Oncology, The Methodist Hospital and Methodist Hospital Research Institute, Houston, Texas (United States); Paulino, Arnold C., E-mail: apaulino@tmhs.org [Department of Radiation Oncology, The Methodist Hospital and Methodist Hospital Research Institute, Houston, Texas (United States)

    2013-03-01

    Patients with soft tissue sarcomas that arise from previously irradiated fields have traditionally been reported to have a poor prognosis. In this report, we examined the characteristics and outcomes of patients who developed a rhabdomyosarcoma in a previously irradiated field (RMS-RIF); we hypothesize that these patients should have a better outcome compared to other postradiation soft tissue sarcomas as these tumors are chemosensitive and radiosensitive. A PubMed search of the literature from 1961-2010 yielded 33 studies with data for patients with RMS-RIF. The study included 43 patients with a median age of 6.5 years at the time of radiation therapy (RT) for the initial tumor. The median RT dose was 48 Gy. The median latency period, the time from RT to development of RMS-RIF, was 8 years. The 3-year overall survival for RMS-RIF was 42%. The 3-year overall survival was 66% for patients receiving chemotherapy and local treatment (surgery and/or RT) compared to 29% for those who had systemic treatment only or local treatment only (P=.049). Other factors associated with increased 3-year overall survival included retinoblastoma initial diagnosis (P<.001), age ≤18 years at diagnosis of RMS-RIF (P=.003), favorable site (P=.008), and stage 1 disease (P=.002). Age at time of RMS-RIF, retinoblastoma initial tumor, favorable site, stage 1 disease, and use of both systemic and local treatment were found to be favorable prognostic factors for 3-year overall survival.

  1. Quality of Standard Reference Materials for Short Time Activation Analysis

    International Nuclear Information System (INIS)

    Ismail, S.S.; Oberleitner, W.

    2003-01-01

    Some environmental reference materials (CFA-1633 b, IAEA-SL-1, SARM-1,BCR-176, Coal-1635, IAEA-SL-3, BCR-146, and SRAM-5) were analysed by short-time activation analysis. The results show that these materials can be classified in three groups, according to their activities after irradiation. The obtained results were compared in order to create a quality index for determination of short-lived nuclides at high count rates. It was found that Cfta is not a suitable standard for determining very short-lived nuclides (half-lives<1 min) because the activity it produces is 15-fold higher than that SL-3. Biological reference materials, such as SRM-1571, SRM-1573, SRM-1575, SRM-1577, IAEA-392, and IAEA-393, were also investigated by a higher counting efficiency system. The quality of this system and its well-type detector for investigating short-lived nuclides was discussed

  2. Analysis on Population Level Reveals Trappability of Wild Rodents Is Determined by Previous Trap Occupant.

    Directory of Open Access Journals (Sweden)

    Marc J Brouard

    Full Text Available Live trapping is central to the study of small mammals. Thus, any bias needs to be understood and accounted for in subsequent analyses to ensure accurate population estimates. One rarely considered bias is the behavioural response of individuals to the trap, in particular the olfactory cues left behind by previous occupants (PO. We used a data set of 8,115 trap nights spanning 17 separate trapping sessions between August 2002 and November 2013 in Wytham Woods, Oxfordshire, UK to examine if the decision to enter a trap was affected by the PO, if this was detectable in traditional Capture-Mark-Recapture trapping data (i.e., individuals not uniquely marked, and if it was possible for this effect to bias the population estimates obtained. Data were collected on Apodemus sylvaticus, Myodes glareolus, and Microtus agrestis. Three Generalised Linear Models revealed a significant tendency for the three species to enter traps with same-species PO. With, for example, A. sylvaticus 9.1 times more likely to enter a same species PO trap compared to one that contained a M. agrestis in the grassland during the nocturnal period. Simulation highlighted that, when all other factors are equal, the species with the highest PO effect will have the highest capture rate and therefore return more accurate population estimates. Despite the large dataset, certain species-, sex-, and/ or age-combinations were under-represented, and thus no effects of any additional individual-specific characteristics could be evaluated. Uniquely marking individuals would allow for the PO effect to be disentangled from other biases such as trap-shyness and spatial heterogeneity, but may not be possible in all cases and will depend on the aims of the study and the resources available.

  3. Possible Detection of Perchlorates by the Sample Analysis at Mars (SAM) Instrument: Comparison with Previous Missions

    Science.gov (United States)

    Navarro-Gonzalex, Rafael; Sutter, Brad; Archer, Doug; Ming, Doug; Eigenbrode, Jennifer; Franz, Heather; Glavin, Daniel; McAdam, Amy; Stern, Jennifer; McKay, Christopher; hide

    2013-01-01

    The first chemical analysis of soluble salts in the soil was carried out by the Phoenix Lander in the Martian Arctic [1]. Surprisingly, chlorine was present as magnesium or calcium perchlorate at 0.4 to 0.6 percent. Additional support for the identification of perchlorate came from the evolved gas analysis which detected the release of molecular oxygen at 350-550C [1]. When Mars-like soils from the Atacama Desert were spiked with magnesium perchlorate (1 percent) and heated using the Viking GC-MS protocol, nearly all the organics were combusted but a small amount was chlorinated, forming chloromethane and dichloromethane [2]. These chlorohydrocarbons were detected by the Viking GC-MS experiments when the Martian soil was analyzed but they were considered to be terrestrial contaminants [3]. Reinterpretation of the Viking results suggests detected compounds (CH3Cl and CH2Cl2) were carried from Earth [4]. Recently the Sample Analysis at Mars (SAM) instrument on board the Mars Science Laboratory (MSL) ran four samples from an aeolian bedform named Rocknest. The samples analyzed were portioned from the fifth scoop at this location. The samples were heated to 835C at 35C/min with a He flow. The SAM QMS detected a major oxygen release (300-500C) [5], coupled with the release of chlorinated hydrocarbons (chloromethane, dichloromethane, trichloromethane, and chloromethylpropene) detected both by SAM QMS and GC-MS derived from known Earth organic contaminants in the instrument [6]. Calcium perchlorate appears to be the best candidate for evolved O2 in the Rocknest samples at this time but other Cl species (e.g., chlorates) are possible and must be evaluated. The potential detection of perchlorates in Rocknest material adds weight to the argument that both Viking Landers measured signatures of perchlorates. Even if the source of the organic carbon detected is still unknown, the chlorine source was likely Martian. Two mechanisms have been hypothesized for the formation of soil

  4. Segmental hair analysis after a single dose of zolpidem: comparison with a previous study.

    Science.gov (United States)

    Cui, Xiaopei; Xiang, Ping; Zhang, Jingshuo; Shi, Yan; Shen, Baohua; Shen, Min

    2013-01-01

    Hair is a useful aid and sometimes even the only matrix in the analytical strategy in drug-facilitated crime (DFC) investigations. In this novel study, segmental hair analysis was performed after a single 10 mg dose of zolpidem was given to 20 Chinese volunteers. Hair was collected 1 month after administration and was analyzed using ultra-high-pressure liquid chromatography/electrospray ionization tandem mass spectrometry. Zolpidem concentrations were found to be in the range of 135.0-554.6 pg/mg in the proximal 0-2 cm segments. These results were markedly different from those reported by Villain et al., who used volunteers administered equal doses of zolpidem. The analytical method used, as well as the volunteers' hair color, inter-individual variations such as metabolic capacity, hair growth rate, drug incorporation rates, physical state of the hair, age, gender, body weight, etc. and diffusion from sweat or other secretions are all factors that should be considered when interpreting the DFC results.

  5. Proteomics analysis in frozen horse mackerel previously high-pressure processed.

    Science.gov (United States)

    Pazos, Manuel; Méndez, Lucía; Vázquez, Manuel; Aubourg, Santiago P

    2015-10-15

    The effect of high-pressure processing (HPP) (150, 300 and 450 MPa for 0, 2.5 and 5 min) on total sodium dodecyl sulphate (SDS)-soluble and sarcoplasmic proteins in frozen (-10 °C for 3 months) horse mackerel (Trachurus trachurus) was evaluated. Proteomics tools based on image analysis of SDS-PAGE protein gels and protein identification by tandem mass spectrometry (MS/MS) were applied. Although total SDS-soluble fraction indicated no important changes induced by HPP, this processing modified the 1-D SDS-PAGE sarcoplasmic patterns in a direct-dependent manner and exerted a selective effect on particular proteins depending on processing conditions. Thus, application of the highest pressure (450 MPa) provoked a significant degradation of phosphoglycerate mutase 2, glycogen phosphorylase muscle form, pyruvate kinase muscle isozyme, beta-enolase and triosephosphate isomerase and phosphoglucomutase-1. Conversely, protein bands assigned to tropomyosin alpha-1 chain, fast myotomal muscle troponin T and parvalbumin beta 2 increased their intensity after applying a 450-MPa processing. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Fate of manuscripts previously rejected by the American Journal of Neuroradiology: a follow-up analysis.

    Science.gov (United States)

    McDonald, R J; Cloft, H J; Kallmes, D F

    2009-02-01

    This is a follow-up article to "Fate of Submitted Manuscripts Rejected from the American Journal of Neuroradiology: Outcomes and Commentary." The purpose of this study was to quantify differences in citation frequency between manuscripts published in the American Journal of Neuroradiology (AJNR) and those published after AJNR rejection and to understand citation frequency differences among rejected manuscripts. In this study, a MEDLINE search identified all manuscripts published in AJNR in 2005 and those initially rejected by AJNR in 2004 but subsequently published elsewhere. Once identified, the citation frequencies of both groups were determined by using SCOPUS. Citation data were used in comparative studies between AJNR rejected and accepted articles and in studies comparing citation frequency differences among rejected manuscripts as a function of journal and/or publication classifications. Among 315 subsequently published rejections from AJNR in 2004, 696 citations accumulated between 2005 and 2007 (2.4 citations/journal year), whereas 441 AJNR articles published in 2005 accumulated 2490 citations between 2005 and 2007 (6.4 citations/journal year, P journals classified as either neuroradiology or general radiology had significantly higher citation frequencies than other submission types and journal classifications. Nonparametric analysis of citation frequency showed significant correlations with impact factors of respective publishing journals (rho = 0.444). Results from this study suggest that publications initially rejected from AJNR have a significantly lower citation frequency than those accepted by AJNR. Among rejected manuscripts, citations frequencies were highest in technical reports and among journals close to the neuroradiologic discipline.

  7. A content analysis of displayed alcohol references on a social networking web site.

    Science.gov (United States)

    Moreno, Megan A; Briner, Leslie R; Williams, Amanda; Brockman, Libby; Walker, Leslie; Christakis, Dimitri A

    2010-08-01

    Exposure to alcohol use in media is associated with adolescent alcohol use. Adolescents frequently display alcohol references on Internet media, such as social networking web sites. The purpose of this study was to conduct a theoretically based content analysis of older adolescents' displayed alcohol references on a social networking web site. We evaluated 400 randomly selected public MySpace profiles of self-reported 17- to 20-year-olds from zip codes, representing urban, suburban, and rural communities in one Washington county. Content was evaluated for alcohol references, suggesting: (1) explicit versus figurative alcohol use, (2) alcohol-related motivations, associations, and consequences, including references that met CRAFFT problem drinking criteria. We compared profiles from four target zip codes for prevalence and frequency of alcohol display. Of 400 profiles, 225 (56.3%) contained 341 references to alcohol. Profile owners who displayed alcohol references were mostly male (54.2%) and white (70.7%). The most frequent reference category was explicit use (49.3%); the most commonly displayed alcohol use motivation was peer pressure (4.7%). Few references met CRAFFT problem drinking criteria (3.2%). There were no differences in prevalence or frequency of alcohol display among the four sociodemographic communities. Despite alcohol use being illegal and potentially stigmatizing in this population, explicit alcohol use is frequently referenced on adolescents' MySpace profiles across several sociodemographic communities. Motivations, associations, and consequences regarding alcohol use referenced on MySpace appear consistent with previous studies of adolescent alcohol use. These references may be a potent source of influence on adolescents, particularly given that they are created and displayed by peers. (c) 2010 Society for Adolescent Health and Medicine. Published by Elsevier Inc. All rights reserved.

  8. Enhanced electricity system analysis for decision making - A reference book

    International Nuclear Information System (INIS)

    2000-01-01

    The objective of electricity system analysis in support of decision making is to provide comparative assessment results upon which relevant policy choices between alternative technology options and supply strategies can be based. This reference book offers analysts, planners and decision makers documented information on enhanced approaches to electricity system analysis, that can assist in achieving this objective. The book describes the main elements of comprehensive electricity system analysis and outlines an advanced integrated analysis and decision making framework for the electric power sector. Emphasis is placed on mechanisms for building consensus between interested and affected parties, and on aspects of planning that go beyond the traditional economic optimisation approach. The scope and contents of the book cover the topics to be addressed in decision making for the power sector and the process of integrating economic, social, health and environmental aspects in the comparative assessment of alternative options and strategies. The book describes and discusses overall frameworks, processes and state of the art methods and techniques available to analysts and planners for carrying out comparative assessment studies, in order to provide sound information to decision makers. This reference book is published as part of a series of technical reports and documents prepared in the framework of the inter-agency joint project (DECADES) on databases and methodologies for comparative assessment of different energy sources for electricity generation. The overall objective of the DECADES project is to enhance capabilities for incorporating economic, social, health and environmental issues in the comparative assessment of electricity generation options and strategies in the process of decision making for the power sector. The project, established in 1992, is carried out jointly by the European Commission (EC), the Economic and Social Commission for Asia and the Pacific

  9. No-Reference Video Quality Assessment using Codec Analysis

    DEFF Research Database (Denmark)

    Søgaard, Jacob; Forchhammer, Søren; Korhonen, Jari

    2015-01-01

    A no-reference video quality assessment (VQA) method is presented for videos distorted by H.264/AVC and MPEG-2. The assessment is performed without access to the bit-stream. Instead we analyze and estimate coefficients based on decoded pixels. The approach involves distinguishing between the two...... types of videos, estimating the level of quantization used in the I-frames, and exploiting this information to assess the video quality. In order to do this for H.264/AVC, the distribution of the DCT-coefficients after intra-prediction and deblocking are modeled. To obtain VQA features for H.264/AVC, we...... propose a novel estimation method of the quantization in H.264/AVC videos without bitstream access, which can also be used for Peak Signalto-Noise Ratio (PSNR) estimation. The results from the MPEG-2 and H.264/AVC analysis are mapped to a perceptual measure of video quality by Support Vector Regression...

  10. Aluminum analysis in biological reference material by nondestructive methods

    International Nuclear Information System (INIS)

    Landsberger, S.; Arendt, A.; Keck, B.; Glascock, M.

    1988-01-01

    In recent years, the determination of aluminum in biological materials has become the subject of many research projects. This interest stems from an increasing knowledge of the toxicity of aluminum to both aquatic and human life. Unfortunately, the detection of aluminum in biological materials has proven troublesome. The use of traditional chemical determinations has been shown to be very long and somewhat complicated. Several attempts have been made using neutron activation analysis, but an interfering reaction must be taken into account. In this experiment the rabbit irradiation facilities at the University of Missouri Research Reactor were used. The aluminum concentrations for eight certified reference materials are shown. When US National Bureau of Standards (NBS) value is given as certified or as an information value, results agree very well. The results for NBS 1572 citrus leaves agree, and NBS 1577 results agree very well with that of Glascock et al

  11. An analysis of uncertainties in the reference resonance absorption calculations

    International Nuclear Information System (INIS)

    Milosevic, M.; Pesic, M.

    1997-05-01

    A recently appeared generation of design-oriented methods, which allows to compute the space and energy dependence of the resonant absorption inside the fuel rod, induces a new problem of validation of results obtained with improved resonance treatments, Because no experimental results are available on the spatial and energy distribution of resonance absorption, detailed reference calculations were generated with the continuos-energy Monte Carlo and energy pointwise slowing-down codes. The accuracy of these calculations depends>on various in.fluences. In this paper an analysis of some influences, such as differences ;n nuclear data libraries and philosophy of reproducing the cross section data, is presented. Example application is given for a calculation benchmark that consists of determination of resonance absorption by 238 U in typical PWR pin cell geometry (author)

  12. Neutron activation analysis of new botanical reference materials. Pt. 2

    International Nuclear Information System (INIS)

    Kucera, J.; Soukal, L.

    1993-01-01

    The certified, information, and other values of elemental contents were compared with results of neutron activation analysis (NAA) for the new Czechoslovak botanical reference materials (RMs) Green Algae 12-02-02, Lucerne 12-02-03, Wheat Bread Fluor 12-02-04, and Rye Bread Flour 12-02-05. These were prepared by the Institute of Radioecology and Applied Nuclear Techniques (IRANT), Kosice, and statistically evaluated after interlaboratory comparisons. For the majority of elements, a very good agreement was found between the IRANT values and the results of NAA. In several cases, however, significant differences were detected; possible analytical reasons for the differences and the suitability of a purely statistical evaluation of intercomparison results without analytical considerations for RM certification are discussed. (orig.)

  13. A fast analysis system for forensic DNA reference samples.

    Science.gov (United States)

    Hedman, Johannes; Albinsson, Linda; Ansell, Carina; Tapper, Helene; Hansson, Oskar; Holgersson, Stig; Ansell, Ricky

    2008-06-01

    On January 1st, 2006, the Swedish legislation on obtaining DNA reference samples from suspects and the recording of DNA profiles in databases was changed. As a result the number of samples analysed at the Swedish National Laboratory of Forensic Science (SKL) increased from about 4500 in 2005 to more than 25,000 in 2006. To meet this challenge, SKL launched a new analysis system to create an unbroken chain, from sampling to incorporation of a profile in the national DNA database and subsequent automatic generation of digitally signed hit reports. The system integrates logistics, digital data transfer, new functions in LIMS (ForumDNA Version 4, Ida Infront AB) and laboratory automation. Buccal swab samples are secured on a FTA card attached to an identity form, which is barcoded with a unique sample ID. After sampling, the police officer sends a digital request to SKL. The sample is automatically registered in LIMS and processed on delivery. The resulting DNA profiles are automatically classified according to quality using a custom-made expert system. Building the evaluation around mathematical rules makes it reproducible, standardised and minimises manual work and clerk errors. All samples are run in duplicate and the two profiles are compared within LIMS before incorporation in the database. In the first year of operation, the median time for completion of an analysis was 3 days, measured from delivery of the sample to incorporation of the profile in the national DNA database. In spite of the dramatic increase in the number of reference samples there was no backlog.

  14. An outline of reference materials for analysis techniques in China

    International Nuclear Information System (INIS)

    Zhang Yuanxun; Qian Yine; Zhang Yongping; Tong Yongpeng

    1994-01-01

    This paper provides background information on the development in the field of reference materials in China. The major considerations in development of reference materials include homogeneity, stability, handling procedures and certification. Further it discusses the plans for development in the near future specific natural-matrix reference materials containing low levels of trace elements and having high degree of homogeneity

  15. Recognizing mild cognitive impairment based on network connectivity analysis of resting EEG with zero reference

    International Nuclear Information System (INIS)

    Xu, Peng; Xiong, Xiu Chun; Tian, Yin; Zhang, Rui; Li, Pei Yang; Yao, De Zhong; Xue, Qing; Wang, Yu Ping; Peng, Yueheng

    2014-01-01

    The diagnosis of mild cognitive impairment (MCI) is very helpful for early therapeutic interventions of Alzheimer's disease (AD). MCI has been proven to be correlated with disorders in multiple brain areas. In this paper, we used information from resting brain networks at different EEG frequency bands to reliably recognize MCI. Because EEG network analysis is influenced by the reference that is used, we also evaluate the effect of the reference choices on the resting scalp EEG network-based MCI differentiation. The conducted study reveals two aspects: (1) the network-based MCI differentiation is superior to the previously reported classification that uses coherence in the EEG; and (2) the used EEG reference influences the differentiation performance, and the zero approximation technique (reference electrode standardization technique, REST) can construct a more accurate scalp EEG network, which results in a higher differentiation accuracy for MCI. This study indicates that the resting scalp EEG-based network analysis could be valuable for MCI recognition in the future. (paper)

  16. Reference indices of hip structural analysis in Ukrainian women

    Directory of Open Access Journals (Sweden)

    N.V. Grygorieva

    2017-10-01

    Full Text Available Background. Nowadays, a comprehensive assessment of osteoporosis and the risk of osteoporotic fractures involves the combine use of bone mineral density (BMD, 10-year probability of major osteoporotic fractures (Fracture Risk Assessment Tool, Trabecular Bone Score, and parameters of hip structural ana­lysis. In recent years, reference data on the three above-mentioned methods have been developed for the Ukrainian population, but there are no data on the latest methodology. The objective of the study was to assess the age characteristics of hip structural analysis parameters in Ukrainian women and to offer their reference values for use in clinical practice. Materials and methods. Using the dual energy X-ray absorptiometry method, we examined 690 healthy women aged 20–89 years wi­thout osteoporosis and other clinically significant diseases and conditions affecting the bone metabolism, without other accompanying pathology of hip joint. Results. The results of the study showed a significant effect of age on femoral strength index (FSI, cross-sectional moment of inertia (CSMI, cross-sectional area (CSA, distance from center of femoral head to center of femoral neck (d1, distance from center of femoral head to inter-trochanteric line (d2, mean femoral neck dia­meter (d3, distance from center of mass of femoral neck to superior neck margin (y, shaft angle (a and hip axis length (HAL indices, but not on parameters of neck/shaft angle (q. A significant decrease of FSI with age was established on the background on increase of CSMI, CSA and HAL parameters. Indices of height and body weight were reliably related with parameters of CSMI, CSA and HAL. FSI was significantly related to the body weight, but not to the height. In addition, it reliably correlated with BMD measured at femoral neck and lesser at total hip and lumbar spine. The HAL did not significant correlate with any of the measured BMD, which confirms its independent role in prediction of

  17. International survey on dietary fiber: definition, analysis, and reference materials.

    Science.gov (United States)

    Lee, S C; Prosky, L

    1995-01-01

    An international survey was conducted to get the views of 147 professionals in the field on the definition of dietary fiber. The survey also solicited opinions on analytical methods for nutrition labeling, quality control, and nutrition research. The survey finds that dietary fiber is generally defined as polysaccharides and lignin that are not hydrolyzed by human alimentary enzymes. Support is strong for expansion of the definition to include oligosaccharides that are resistant to hydrolysis by human alimentary enzymes. Among techniques for nutrition labeling and quality control, enzymatic-gravimetric methods get the highest support. For nutrition research, more detailed methods such as gas-liquid chromatography and liquid chromatography were considered more appropriate. Respondents support labeling of total, soluble, and insoluble dietary fiber or total dietary fiber alone as sufficient for nutrition labeling of food packages. However, for nutrition research, detailed analytical methods, improvements in accuracy (i.e., closer simulation of in vitro techniques to conditions of human gastrointestinal tract), and improvements in precision and simplicity are suggested. Less than 20% of the participants use reference materials for dietary fiber analysis.

  18. Nuclear microprobe analysis of the standard reference materials

    International Nuclear Information System (INIS)

    Jaksic, M.; Fazinic, S.; Bogdanovic, I.; Tadic, T.

    2002-01-01

    Most of the presently existing Standard Reference Materials (SRM) for nuclear analytical methods are certified for the analyzed mass of the order of few hundred mg. Typical mass of sample which is analyzed by PIXE or XRF methods is very often below 1 mg. By the development of focused proton or x-ray beams, masses which can be typically analyzed go down to μg or even ng level. It is difficult to make biological or environmental SRMs which can give desired homogeneity at such low scale. However, use of fundamental parameter quantitative evaluation procedures (absolute method), minimize needs for SRMs. In PIXE and micro PIXE setup at our Institute, fundamental parameter approach is used. For exact calibration of the quantitative analysis procedure just one standard sample is needed. In our case glass standards which showed homogeneity down to micron scale were used. Of course, it is desirable to use SRMs for quality assurance, and therefore need for homogenous materials can be justified even for micro PIXE method. In this presentation, brief overview of PIXE setup calibration is given, along with some recent results of tests of several SRMs

  19. Arab Americans: A Comparative Critical Analysis of Leading Reference Sources.

    Science.gov (United States)

    Wertsman, Vladimir F.

    2001-01-01

    Examines eight reference sources on Arab Americans to investigate how complete the information is on various facets of Arab American presence, history, community life, heritage preservation, organizations, and the press, concluding that the reference sources complement each other, but taken together, they do not cover the entire multidimensional…

  20. Use of gamma spectrometry for analysis of three reference materials

    International Nuclear Information System (INIS)

    Kinova, L.

    2004-01-01

    All reference materials (Reference material A: weight = 49.23 g; Reference material B: weight = 36.08 g; Reference material C: weight = 26.18 g) were packed in 50 cm 3 polypropylene vials, sealed and measured consecutively three times at intervals of the average of 25 days. Low background gamma spectrometry system: HPGe detector with high energy resolution (FWHM for 1332 KeV of Co-60 is 1.9 KeV, Relative counting efficiency for the same energy is 21 %) was used. Results: All materials are of low activity and must be measured for a long time.The highest specific activity of a man-made radionuclides Cs-137 and Am-241 is in the material A. An instrumentally measurable activity of Pb-210 also can be observed in this material. Medium values are in the material B. The reference material C according to the specific activity seems to be a low natural radioactivity material with highest activity of natural nuclides Th-232 and Pa-234 (progeny of U-238). Conclusions: Gamma spectrometry is an useful tool for initial measurement of materials with low radioactivity. Such measurements give an orientation for the nuclides content and approximate activity in the material for the following radiochemical determinations

  1. At what price? A cost-effectiveness analysis comparing trial of labour after previous caesarean versus elective repeat caesarean delivery.

    Directory of Open Access Journals (Sweden)

    Christopher G Fawsitt

    Full Text Available BACKGROUND: Elective repeat caesarean delivery (ERCD rates have been increasing worldwide, thus prompting obstetric discourse on the risks and benefits for the mother and infant. Yet, these increasing rates also have major economic implications for the health care system. Given the dearth of information on the cost-effectiveness related to mode of delivery, the aim of this paper was to perform an economic evaluation on the costs and short-term maternal health consequences associated with a trial of labour after one previous caesarean delivery compared with ERCD for low risk women in Ireland. METHODS: Using a decision analytic model, a cost-effectiveness analysis (CEA was performed where the measure of health gain was quality-adjusted life years (QALYs over a six-week time horizon. A review of international literature was conducted to derive representative estimates of adverse maternal health outcomes following a trial of labour after caesarean (TOLAC and ERCD. Delivery/procedure costs derived from primary data collection and combined both "bottom-up" and "top-down" costing estimations. RESULTS: Maternal morbidities emerged in twice as many cases in the TOLAC group than the ERCD group. However, a TOLAC was found to be the most-effective method of delivery because it was substantially less expensive than ERCD (€ 1,835.06 versus € 4,039.87 per women, respectively, and QALYs were modestly higher (0.84 versus 0.70. Our findings were supported by probabilistic sensitivity analysis. CONCLUSIONS: Clinicians need to be well informed of the benefits and risks of TOLAC among low risk women. Ideally, clinician-patient discourse would address differences in length of hospital stay and postpartum recovery time. While it is premature advocate a policy of TOLAC across maternity units, the results of the study prompt further analysis and repeat iterations, encouraging future studies to synthesis previous research and new and relevant evidence under a single

  2. Content Analysis of Virtual Reference Data: Reshaping Library Website Design.

    Science.gov (United States)

    Fan, Suhua Caroline; Welch, Jennifer M

    2016-01-01

    An academic health sciences library wanted to redesign its website to provide better access to health information in the community. Virtual reference data were used to provide information about user searching behavior. This study analyzed three years (2012-2014) of virtual reference data, including e-mail questions, text messaging, and live chat transcripts, to evaluate the library website for redesigning, especially in areas such as the home page, patrons' terminology, and issues prompting patrons to ask for help. A coding system based on information links in the current library website was created to analyze the data.

  3. No-Reference Video Quality Assessment by HEVC Codec Analysis

    DEFF Research Database (Denmark)

    Huang, Xin; Søgaard, Jacob; Forchhammer, Søren

    2015-01-01

    This paper proposes a No-Reference (NR) Video Quality Assessment (VQA) method for videos subject to the distortion given by High Efficiency Video Coding (HEVC). The proposed assessment can be performed either as a BitstreamBased (BB) method or as a Pixel-Based (PB). It extracts or estimates the t...

  4. Sensitivity analysis of reference evapotranspiration to sensor accuracy

    Science.gov (United States)

    Meteorological sensor networks are often used across agricultural regions to calculate the ASCE Standardized Reference ET Equation, and inaccuracies in individual sensors can lead to inaccuracies in ET estimates. Multiyear datasets from the semi-arid Colorado Agricultural Meteorological (CoAgMet) an...

  5. Student Teacher Letters of Reference: A Critical Analysis

    Science.gov (United States)

    Mason, Richard W.; Schroeder, Mark P.

    2012-01-01

    Letters of reference are commonly used in acquiring a job in education. Despite serious issues of validity and reliability in writing and evaluating letters, there is a dearth of research that systematically examines the evaluation process and defines the constructs that define high quality letters. The current study used NVivo to examine 160…

  6. Lower Columbia River and Estuary Ecosystem Restoration Program Reference Site Study: 2011 Restoration Analysis - FINAL REPORT

    Energy Technology Data Exchange (ETDEWEB)

    Borde, Amy B.; Cullinan, Valerie I.; Diefenderfer, Heida L.; Thom, Ronald M.; Kaufmann, Ronald M.; Zimmerman, Shon A.; Sagar, Jina; Buenau, Kate E.; Corbett, C.

    2012-05-31

    The Reference Site (RS) study is part of the research, monitoring, and evaluation (RME) effort developed by the Action Agencies (Bonneville Power Administration [BPA], U.S. Army Corps of Engineers, Portland District [USACE], and U.S. Bureau of Reclamation) in response to Federal Columbia River Power System (FCRPS) Biological Opinions (BiOp). While the RS study was initiated in 2007, data have been collected at relatively undisturbed reference wetland sites in the LCRE by PNNL and collaborators since 2005. These data on habitat structural metrics were previously summarized to provide baseline characterization of 51 wetlands throughout the estuarine and tidal freshwater portions of the 235-km LCRE; however, further analysis of these data has been limited. Therefore, in 2011, we conducted additional analyses of existing field data previously collected for the Columbia Estuary Ecosystem Restoration Program (CEERP) - including data collected by PNNL and others - to help inform the multi-agency restoration planning and ecosystem management work underway in the LCRE.

  7. Analysis of Periodic Errors for Synthesized-Reference-Wave Holography

    Directory of Open Access Journals (Sweden)

    V. Schejbal

    2009-12-01

    Full Text Available Synthesized-reference-wave holographic techniques offer relatively simple and cost-effective measurement of antenna radiation characteristics and reconstruction of complex aperture fields using near-field intensity-pattern measurement. These methods allow utilization of advantages of methods for probe compensations for amplitude and phasing near-field measurements for the planar and cylindrical scanning including accuracy analyses. The paper analyzes periodic errors, which can be created during scanning, using both theoretical results and numerical simulations.

  8. Testing and reference model analysis of FTTH system

    Science.gov (United States)

    Feng, Xiancheng; Cui, Wanlong; Chen, Ying

    2009-08-01

    With rapid development of Internet and broadband access network, the technologies of xDSL, FTTx+LAN , WLAN have more applications, new network service emerges in endless stream, especially the increase of network game, meeting TV, video on demand, etc. FTTH supports all present and future service with enormous bandwidth, including traditional telecommunication service, traditional data service and traditional TV service, and the future digital TV and VOD. With huge bandwidth of FTTH, it wins the final solution of broadband network, becomes the final goal of development of optical access network.. Fiber to the Home (FTTH) will be the goal of telecommunications cable broadband access. In accordance with the development trend of telecommunication services, to enhance the capacity of integrated access network, to achieve triple-play (voice, data, image), based on the existing optical Fiber to the curb (FTTC), Fiber To The Zone (FTTZ), Fiber to the Building (FTTB) user optical cable network, the optical fiber can extend to the FTTH system of end-user by using EPON technology. The article first introduced the basic components of FTTH system; and then explain the reference model and reference point for testing of the FTTH system; Finally, by testing connection diagram, the testing process, expected results, primarily analyze SNI Interface Testing, PON interface testing, Ethernet performance testing, UNI interface testing, Ethernet functional testing, PON functional testing, equipment functional testing, telephone functional testing, operational support capability testing and so on testing of FTTH system. ...

  9. A novel micro-structured reference material for microbeam analysis

    Science.gov (United States)

    Wätjen, Uwe; Dücsö, Csaba; Tajani, Antonella; Munnik, Frans; Lechtenberg, Frank

    2000-03-01

    In order to determine the beam spot size and the linear or raster scanning properties of microprobe analytical systems, a novel certified reference material (CRM) has been developed by IRMM, consisting of permalloy (81% Ni, 19% Fe) strip patterns of different widths on a silicon substrate. The general layout of this micro-structured reference material with pattern sizes ranging from 2 to 100 μm, fabricated with production schemes of microelectronics circuitry on silicon wafers, is discussed. The large size range of the individual pattern structures makes the material equally applicable to very fine and less focused microbeams. Several long distances between characteristic patterns as well as broad line widths of selected structures are certified for each individual chip. First chips of this material were investigated with high-energy ion microprobes as well as with X-ray microprobes with capillary optics. Due to the very good definition of the metal lines and their edge profiles, line scan results of XRF, PIXE or RBS can be directly converted to spot size and microbeam profile. A special set of micro-structures on the CRM chips allows to obtain quantitative information about the "skirt" of microbeams.

  10. Market segmentation based on consumers’ susceptibility to reference group types of influence: Multivariance analysis

    Directory of Open Access Journals (Sweden)

    Mirela Mihić

    2006-12-01

    Full Text Available In this paper we begin with McGuire’s concept of influenceability, according to which individuals differ based on their susceptibility to social influence. The theoretical part explains three types of influence by reference groups and presents previous results relevant to the issue of this paper. The second part of the paper presents the methodology and research results. The aim of this research is to identify different types of reference group influence by using multivariance techniques, and determine whether they can serve as a basis for consumer market segmentation. The research was conducted on a sample of 250 respondents in the Split-Dalmatia County. Keeping in mind the issues and goals of the research, two hypotheses were set. Five factors – influence types were identified by using the factor analysis (normative influence, value-expressive or identificational influence, environment informative influence, salesperson’s informative influence, and comparison to environment and clothing conformity, and were then been used as basic segmentation variables. Cluster analysis singled out three segments: subject to identification or value-expressive influence, subject to information influence and non-subject to influence. To describe them better, demographic variables were employed, i.e. “relation-comparison and interaction with others” variables as well as personal indicators. The research results confirmed both starting hypotheses. The results attained suggest that consumers from particular segments require different communication strategies, based on which, each segment was supported by corresponding recommendations.

  11. NMR-based phytochemical analysis of Vitis vinifera cv Falanghina leaves. Characterization of a previously undescribed biflavonoid with antiproliferative activity.

    Science.gov (United States)

    Tartaglione, Luciana; Gambuti, Angelita; De Cicco, Paola; Ercolano, Giuseppe; Ianaro, Angela; Taglialatela-Scafati, Orazio; Moio, Luigi; Forino, Martino

    2018-03-01

    Vitis vinifera cv Falanghina is an ancient grape variety of Southern Italy. A thorough phytochemical analysis of the Falanghina leaves was conducted to investigate its specialised metabolite content. Along with already known molecules, such as caftaric acid, quercetin-3-O-β-d-glucopyranoside, quercetin-3-O-β-d-glucuronide, kaempferol-3-O-β-d-glucopyranoside and kaempferol-3-O-β-d-glucuronide, a previously undescribed biflavonoid was identified. For this last compound, a moderate bioactivity against metastatic melanoma cells proliferation was discovered. This datum can be of some interest to researchers studying human melanoma. The high content in antioxidant glycosylated flavonoids supports the exploitation of grape vine leaves as an inexpensive source of natural products for the food industry and for both pharmaceutical and nutraceutical companies. Additionally, this study offers important insights into the plant physiology, thus prompting possible technological researches of genetic selection based on the vine adaptation to specific pedo-climatic environments. Copyright © 2017 Elsevier B.V. All rights reserved.

  12. No-Reference Video Quality Assessment using MPEG Analysis

    DEFF Research Database (Denmark)

    Søgaard, Jacob; Forchhammer, Søren; Korhonen, Jari

    2013-01-01

    and estimate the video coding parameters for MPEG-2 and H.264/AVC which can be used to improve the VQA. The analysis differs from most other video coding analysis methods since it is without access to the bitstream. The results show that our proposed method is competitive with other recent NR VQA methods...... for MPEG-2 and H.264/AVC....

  13. No-Reference Video Quality Assessment by HEVC Codec Analysis

    DEFF Research Database (Denmark)

    Huang, Xin; Søgaard, Jacob; Forchhammer, Søren

    2015-01-01

    This paper proposes a No-Reference (NR) Video Quality Assessment (VQA) method for videos subject to the distortion given by High Efficiency Video Coding (HEVC). The proposed assessment can be performed either as a BitstreamBased (BB) method or as a Pixel-Based (PB). It extracts or estimates...... the transform coefficients, estimates the distortion, and assesses the video quality. The proposed scheme generates VQA features based on Intra coded frames, and then maps features using an Elastic Net to predict subjective video quality. A set of HEVC coded 4K UHD sequences are tested. Results show...... that the quality scores computed by the proposed method are highly correlated with the subjective assessment....

  14. Reference values for analysis of peritoneal fluid of healthy equines

    OpenAIRE

    Neves, Mariana Machado; Marques Jr., Antônio de Pinho; Alves, Geraldo Eleno Silveira; Faria, Evenilde Picardi

    2000-01-01

    Considerando a casuística de distúrbios abdominais em eqüinos, e o fato da paracentese ser uma técnica fácil e segura para o animal, a análise do líquido peritoneal torna-se um importante exame auxiliar no diagnóstico e direcionamento da conduta clínica. Como no Brasil não existem valores de referência desse exame em eqüinos sem raça definida, este trabalho objetivou o estabelecimento desses valores para esses animais. Foram usados sessenta eqüinos sem raça definida, procedentes da Região Met...

  15. Summary Report: First Research Coordination Meeting on ReferenceDatabase for Neutron Activation Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Firestone, Richard B.; Trkov, Andrej

    2005-10-01

    Potential problems associated with nuclear data for neutronactivation analysis were identified, the scope of the work to beundertaken was defined together with its priorities, and tasks wereassigned to participants. Data testing and measurements refer to gammaspectrumpeak evaluations, detector efficiency calibration, neutronspectrum characteristics and reference materials analysis.

  16. Analysis of Numerical Weather Predictions of Reference Evapotranspiration and Precipitation

    Science.gov (United States)

    Bughici, Theodor; Lazarovitch, Naftali; Fredj, Erick; Tas, Eran

    2017-04-01

    This study attempts to improve the forecast skill of the evapotranspiration (ET0) and Precipitation for the purpose of crop irrigation management over Israel using the Weather Research and Forecasting (WRF) Model. Optimized crop irrigation, in term of timing and quantities, decreases water and agrochemicals demand. Crop water demands depend on evapotranspiration and precipitation. The common method for computing reference evapotranspiration, for agricultural needs, ET0, is according to the FAO Penman-Monteith equation. The weather variables required for ET0 calculation (air temperature, relative humidity, wind speed and solar irradiance) are estimated by the WRF model. The WRF Model with two-way interacting domains at horizontal resolutions of 27, 9 and 3 km is used in the study. The model prediction was performed in an hourly time resolution and a 3 km spatial resolution, with forecast lead-time of up to four days. The WRF prediction of these variables have been compared against measurements from 29 meteorological stations across Israel for the year 2013. The studied area is small but with strong climatic gradient, diverse topography and variety of synoptic conditions. The forecast skill that was used for forecast validation takes into account the prediction bias, mean absolute error and root mean squared error. The forecast skill of the variables was almost robust to lead time, except for precipitation. The forecast skill was tested across stations with respect to topography and geographic location and for all stations with respect to seasonality and synoptic weather system determined by employing a semi-objective synoptic systems classification to the forecasted days. It was noticeable that forecast skill of some of the variables was deteriorated by seasonality and topography. However, larger impacts in the ET0 skill scores on the forecasted day are achieved by a synoptic based forecast. These results set the basis for increasing the robustness of ET0 to

  17. Example Work Domain Analysis for a Reference Sodium Fast Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Hugo, Jacques [Idaho National Lab. (INL), Idaho Falls, ID (United States); Oxstrand, Johanna [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-01-01

    The nuclear industry is currently designing and building a new generation of reactors that will include different structural, functional, and environmental aspects, all of which are likely to have a significant impact on the way these plants are operated. In order to meet economic and safety objectives, these new reactors will all use advanced technologies to some extent, including new materials and advanced digital instrumentation and control systems. New technologies will affect not only operational strategies, but will also require a new approach to how functions are allocated to humans or machines to ensure optimal performance. Uncertainty about the effect of large scale changes in plant design will remain until sound technical bases are developed for new operational concepts and strategies. Up-to-date models and guidance are required for the development of operational concepts for complex socio-technical systems. This report describes how the classical Work Domain Analysis method was adapted to develop operational concept frameworks for new plants. This adaptation of the method is better able to deal with the uncertainty and incomplete information typical of first-of-a-kind designs. Practical examples are provided of the systematic application of the method in the operational analysis of sodium-cooled reactors. Insights from this application and its utility are reviewed and arguments for the formal adoption of Work Domain Analysis as a value-added part of the Systems Engineering process are presented.

  18. Reference respiratory waveforms by minimum jerk model analysis

    Energy Technology Data Exchange (ETDEWEB)

    Anetai, Yusuke, E-mail: anetai@radonc.med.osaka-u.ac.jp; Sumida, Iori; Takahashi, Yutaka; Yagi, Masashi; Mizuno, Hirokazu; Ogawa, Kazuhiko [Department of Radiation Oncology, Osaka University Graduate School of Medicine, Yamadaoka 2-2, Suita-shi, Osaka 565-0871 (Japan); Ota, Seiichi [Department of Medical Technology, Osaka University Hospital, Yamadaoka 2-15, Suita-shi, Osaka 565-0871 (Japan)

    2015-09-15

    Purpose: CyberKnife{sup ®} robotic surgery system has the ability to deliver radiation to a tumor subject to respiratory movements using Synchrony{sup ®} mode with less than 2 mm tracking accuracy. However, rapid and rough motion tracking causes mechanical tracking errors and puts mechanical stress on the robotic joint, leading to unexpected radiation delivery errors. During clinical treatment, patient respiratory motions are much more complicated, suggesting the need for patient-specific modeling of respiratory motion. The purpose of this study was to propose a novel method that provides a reference respiratory wave to enable smooth tracking for each patient. Methods: The minimum jerk model, which mathematically derives smoothness by means of jerk, or the third derivative of position and the derivative of acceleration with respect to time that is proportional to the time rate of force changed was introduced to model a patient-specific respiratory motion wave to provide smooth motion tracking using CyberKnife{sup ®}. To verify that patient-specific minimum jerk respiratory waves were being tracked smoothly by Synchrony{sup ®} mode, a tracking laser projection from CyberKnife{sup ®} was optically analyzed every 0.1 s using a webcam and a calibrated grid on a motion phantom whose motion was in accordance with three pattern waves (cosine, typical free-breathing, and minimum jerk theoretical wave models) for the clinically relevant superior–inferior directions from six volunteers assessed on the same node of the same isocentric plan. Results: Tracking discrepancy from the center of the grid to the beam projection was evaluated. The minimum jerk theoretical wave reduced the maximum-peak amplitude of radial tracking discrepancy compared with that of the waveforms modeled by cosine and typical free-breathing model by 22% and 35%, respectively, and provided smooth tracking for radial direction. Motion tracking constancy as indicated by radial tracking discrepancy

  19. Analysis and influences of reference systems in the shoulder kinematics

    OpenAIRE

    Gutiérrez, G.; Ortega, D.R.; Iznaga, A.M.; Beule, M.; Verhegghe, B.; Tongel, A.; Palmans, T.; Hoof, T.; Rodríguez, T.; Segers, P.

    2017-01-01

    The shoulder is one of the most complex systems in the human body. To know how its work is essential to understand the biomechanics of the body. The purpose of this study was to analyze the influence of Local Coordinate Systems in the kinematic analysis of GlenoHumeral joint. The motion tracking was performed using passive sensors attached to pins. Trabecular pins were placed on the shoulder and scapula. The data processing was performed using the free open software pyFormex. The joint displa...

  20. Analysis of 60 706 Exomes Questions the Role of De Novo Variants Previously Implicated in Cardiac Disease

    DEFF Research Database (Denmark)

    Paludan-Müller, Christian; Ahlberg, Gustav; Ghouse, Jonas

    2017-01-01

    sought to evaluate the pathogenicity of de novo variants previously associated with cardiac disease based on a large population-representative exome database. METHODS AND RESULTS: We performed a literature search for previous publications on de novo variants associated with severe arrhythmias...... and structural heart diseases and investigated whether these variants were present in the Exome Aggregation Consortium (ExAC) database (n=60 706). We identified monogenic variants in single case reports and smaller studies (≤200 subjects) and variants considered to increase susceptibility of disease in 3 larger...

  1. Analysis of over 10,000 Cases finds no association between previously reported candidate polymorphisms and ovarian cancer outcome

    DEFF Research Database (Denmark)

    White, Kristin L; Vierkant, Robert A; Fogarty, Zachary C

    2013-01-01

    Ovarian cancer is a leading cause of cancer-related death among women. In an effort to understand contributors to disease outcome, we evaluated single-nucleotide polymorphisms (SNP) previously associated with ovarian cancer recurrence or survival, specifically in angiogenesis, inflammation, mitosis...

  2. The relationship between emotional intelligence, previous caring experience and mindfulness in student nurses and midwives: a cross sectional analysis.

    Science.gov (United States)

    Snowden, Austyn; Stenhouse, Rosie; Young, Jenny; Carver, Hannah; Carver, Fiona; Brown, Norrie

    2015-01-01

    Emotional Intelligence (EI), previous caring experience and mindfulness training may have a positive impact on nurse education. More evidence is needed to support the use of these variables in nurse recruitment and retention. To explore the relationship between EI, gender, age, programme of study, previous caring experience and mindfulness training. Cross sectional element of longitudinal study. 938year one nursing, midwifery and computing students at two Scottish Higher Education Institutes (HEIs) who entered their programme in September 2013. Participants completed a measure of 'trait' EI: Trait Emotional Intelligence Questionnaire Short Form (TEIQue-SF); and 'ability' EI: Schutte's et al. (1998) Emotional Intelligence Scale (SEIS). Demographics, previous caring experience and previous training in mindfulness were recorded. Relationships between variables were tested using non-parametric tests. Emotional intelligence increased with age on both measures of EI [TEIQ-SF H(5)=15.157 p=0.001; SEIS H(5)=11.388, p=0.044]. Females (n=786) scored higher than males (n=149) on both measures [TEIQ-SF, U=44,931, z=-4.509, pintelligence. Mindfulness training was associated with higher 'ability' emotional intelligence. Implications for recruitment, retention and further research are explored. Copyright © 2014. Published by Elsevier Ltd.

  3. Is popular radio a source of exposure to alcohol references in mid to later life? A content analysis.

    Science.gov (United States)

    Haighton, C; Halligan, J; Scott, S

    2017-04-20

    There is concern around alcohol consumption in mid to later life yet little understanding about what influences this behaviour. No previous research has explored the extent to which adults in mid to later life may be exposed to alcohol references in the media. This project aimed to determine the frequency of alcohol references on radio stations with a high proportion of listeners in mid to later life. Content analysis of alcohol references on four popular UK music-based radio stations with a high proportion of listeners aged 55-64 years over three time points. Alcohol references occur frequently, but vary by time of year and type of radio station. When alcohol is mentioned its consumption is portrayed as the norm, without negative consequences. On three commercial stations, the majority of mentions came from advertising, whereas on BBC Radio 2 nearly all references were talk-based. All adverts for direct promotion of alcohol were by supermarkets. Alcohol was frequently associated with celebrations, socializing or something to consume for its own sake. Adults in the age group 55-64 may be exposed to references to alcohol that could serve to reinforce norms of consumption of alcohol and promote purchases of cheap alcohol. © The Author 2017. Published by Oxford University Press on behalf of Faculty of Public Health. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

  4. What place is this time? Semiotics and the analysis of historical reference in landschape architecture

    NARCIS (Netherlands)

    Assche, van K.A.M.; Duineveld, M.; Jong, de H.C.; Zoest, van A.

    2012-01-01

    This paper revisits the potential contribution of semiotic analysis to heritage design. A case study analyzes lay interpretations of a number of urban landscape designs, displaying different ways to refer to the invisible (archaeological) past. A total of 12 draft designs were produced referring to

  5. HYBRID SULFUR PROCESS REFERENCE DESIGN AND COST ANALYSIS

    Energy Technology Data Exchange (ETDEWEB)

    Gorensek, M.; Summers, W.; Boltrunis, C.; Lahoda, E.; Allen, D.; Greyvenstein, R.

    2009-05-12

    This report documents a detailed study to determine the expected efficiency and product costs for producing hydrogen via water-splitting using energy from an advanced nuclear reactor. It was determined that the overall efficiency from nuclear heat to hydrogen is high, and the cost of hydrogen is competitive under a high energy cost scenario. It would require over 40% more nuclear energy to generate an equivalent amount of hydrogen using conventional water-cooled nuclear reactors combined with water electrolysis compared to the proposed plant design described herein. There is a great deal of interest worldwide in reducing dependence on fossil fuels, while also minimizing the impact of the energy sector on global climate change. One potential opportunity to contribute to this effort is to replace the use of fossil fuels for hydrogen production by the use of water-splitting powered by nuclear energy. Hydrogen production is required for fertilizer (e.g. ammonia) production, oil refining, synfuels production, and other important industrial applications. It is typically produced by reacting natural gas, naphtha or coal with steam, which consumes significant amounts of energy and produces carbon dioxide as a byproduct. In the future, hydrogen could also be used as a transportation fuel, replacing petroleum. New processes are being developed that would permit hydrogen to be produced from water using only heat or a combination of heat and electricity produced by advanced, high temperature nuclear reactors. The U.S. Department of Energy (DOE) is developing these processes under a program known as the Nuclear Hydrogen Initiative (NHI). The Republic of South Africa (RSA) also is interested in developing advanced high temperature nuclear reactors and related chemical processes that could produce hydrogen fuel via water-splitting. This report focuses on the analysis of a nuclear hydrogen production system that combines the Pebble Bed Modular Reactor (PBMR), under development by

  6. Dispersion analysis for baseline reference mission 1. [flight simulation and trajectory analysis for space shuttle orbiter

    Science.gov (United States)

    Kuhn, A. E.

    1975-01-01

    A dispersion analysis considering 3 sigma uncertainties (or perturbations) in platform, vehicle, and environmental parameters was performed for the baseline reference mission (BRM) 1 of the space shuttle orbiter. The dispersion analysis is based on the nominal trajectory for the BRM 1. State vector and performance dispersions (or variations) which result from the indicated 3 sigma uncertainties were studied. The dispersions were determined at major mission events and fixed times from lift-off (time slices) and the results will be used to evaluate the capability of the vehicle to perform the mission within a 3 sigma level of confidence and to determine flight performance reserves. A computer program is given that was used for dynamic flight simulations of the space shuttle orbiter.

  7. Data Link Test and Analysis System/ATCRBS Transponder Test System Technical Reference

    Science.gov (United States)

    1990-05-01

    This document references material for personnel using or making software changes : to the Data Link Test and Analysis System (DATAS) for Air Traffic Control Radar : Beacon System (ATCRBS) transponder testing and data collection. This is one of : a se...

  8. Instrumental neutron activation analysis of marine sediment in-house reference material

    International Nuclear Information System (INIS)

    Nazaratul Ashifa Abdullah Salim; Mohd Suhaimi Hamzah; Mohd Suhaimi Elias; Siong, W.B.; Shamsiah Abdul Rahman; Azian Hashim; Shakirah Abdul Shukor

    2013-01-01

    Reference materials play an important role in demonstrating the quality and reliability of analytical data. The advantage of using in-house reference materials is that they provide a relatively cheap option as compared to using commercially available certified reference material (CRM) and can closely resemble the laboratory routine test sample. A marine sediment sample was designed as an in-house reference material, in the framework of quality assurance and control (QA/QC) program of the Neutron Activation Analysis (NAA) Laboratory at Nuclear Malaysia. The NAA technique was solely used for the homogeneity test of the marine sediment sample. The CRM of IAEA- Soil 7 and IAEA- SL1 (Lake Sediment) were applied in the analysis as compatible matrix based reference materials for QA purposes. (Author)

  9. At what price? A cost-effectiveness analysis comparing trial of labour after previous Caesarean versus elective repeat Caesarean delivery.

    LENUS (Irish Health Repository)

    Fawsitt, Christopher G

    2013-01-01

    Elective repeat caesarean delivery (ERCD) rates have been increasing worldwide, thus prompting obstetric discourse on the risks and benefits for the mother and infant. Yet, these increasing rates also have major economic implications for the health care system. Given the dearth of information on the cost-effectiveness related to mode of delivery, the aim of this paper was to perform an economic evaluation on the costs and short-term maternal health consequences associated with a trial of labour after one previous caesarean delivery compared with ERCD for low risk women in Ireland.

  10. Analysis of Over 10,000 Cases Finds No Association between Previously-Reported Candidate Polymorphisms and Ovarian Cancer Outcome

    Science.gov (United States)

    White, Kristin L.; Vierkant, Robert A.; Fogarty, Zachary C.; Charbonneau, Bridget; Block, Matthew S.; Pharoah, Paul D.P.; Chenevix-Trench, Georgia; Rossing, Mary Anne; Cramer, Daniel W.; Pearce, C. Leigh; Schildkraut, Joellen M.; Menon, Usha; Kjaer, Susanne Kruger; Levine, Douglas A.; Gronwald, Jacek; Culver, Hoda Anton; Whittemore, Alice S.; Karlan, Beth Y.; Lambrechts, Diether; Wentzensen, Nicolas; Kupryjanczyk, Jolanta; Chang-Claude, Jenny; Bandera, Elisa V.; Hogdall, Estrid; Heitz, Florian; Kaye, Stanley B.; Fasching, Peter A.; Campbell, Ian; Goodman, Marc T.; Pejovic, Tanja; Bean, Yukie; Lurie, Galina; Eccles, Diana; Hein, Alexander; Beckmann, Matthias W.; Ekici, Arif B.; Paul, James; Brown, Robert; Flanagan, James; Harter, Philipp; du Bois, Andreas; Schwaab, Ira; Hogdall, Claus K.; Lundvall, Lene; Olson, Sara H.; Orlow, Irene; Paddock, Lisa E.; Rudolph, Anja; Eilber, Ursula; Dansonka-Mieszkowska, Agnieszka; Rzepecka, Iwona K.; Ziolkowska-Seta, Izabela; Brinton, Louise; Yang, Hannah; Garcia-Closas, Montserrat; Despierre, Evelyn; Lambrechts, Sandrina; Vergote, Ignace; Walsh, Christine; Lester, Jenny; Sieh, Weiva; McGuire, Valerie; Rothstein, Joseph H.; Ziogas, Argyrios; Lubiński, Jan; Cybulski, Cezary; Menkiszak, Janusz; Jensen, Allan; Gayther, Simon A.; Ramus, Susan J.; Gentry-Maharaj, Aleksandra; Berchuck, Andrew; Wu, Anna H.; Pike, Malcolm C.; Van Den Berg, David; Terry, Kathryn L.; Vitonis, Allison F.; Doherty, Jennifer A.; Johnatty, Sharon; deFazio, Anna; Song, Honglin; Tyrer, Jonathan; Sellers, Thomas A.; Phelan, Catherine M.; Kalli, Kimberly R.; Cunningham, Julie M.; Fridley, Brooke L.; Goode, Ellen L.

    2013-01-01

    Background Ovarian cancer is a leading cause of cancer-related death among women. In an effort to understand contributors to disease outcome, we evaluated single-nucleotide polymorphisms (SNPs) previously associated with ovarian cancer recurrence or survival, specifically in angiogenesis, inflammation, mitosis, and drug disposition genes. Methods Twenty-seven SNPs in VHL, HGF, IL18, PRKACB, ABCB1, CYP2C8, ERCC2, and ERCC1 previously associated with ovarian cancer outcome were genotyped in 10,084 invasive cases from 28 studies from the Ovarian Cancer Association Consortium with over 37,000 observed person-years and 4,478 deaths. Cox proportional hazards models were used to examine the association between candidate SNPs and ovarian cancer recurrence or survival with and without adjustment for key covariates. Results We observed no association between genotype and ovarian cancer recurrence or survival for any of the SNPs examined. Conclusions These results refute prior associations between these SNPs and ovarian cancer outcome and underscore the importance of maximally powered genetic association studies. Impact These variants should not be used in prognostic models. Alternate approaches to uncovering inherited prognostic factors, if they exist, are needed. PMID:23513043

  11. Analysis of over 10,000 Cases finds no association between previously reported candidate polymorphisms and ovarian cancer outcome.

    Science.gov (United States)

    White, Kristin L; Vierkant, Robert A; Fogarty, Zachary C; Charbonneau, Bridget; Block, Matthew S; Pharoah, Paul D P; Chenevix-Trench, Georgia; Rossing, Mary Anne; Cramer, Daniel W; Pearce, Celeste Leigh; Schildkraut, Joellen M; Menon, Usha; Kjaer, Susanne Kruger; Levine, Douglas A; Gronwald, Jacek; Culver, Hoda Anton; Whittemore, Alice S; Karlan, Beth Y; Lambrechts, Diether; Wentzensen, Nicolas; Kupryjanczyk, Jolanta; Chang-Claude, Jenny; Bandera, Elisa V; Hogdall, Estrid; Heitz, Florian; Kaye, Stanley B; Fasching, Peter A; Campbell, Ian; Goodman, Marc T; Pejovic, Tanja; Bean, Yukie; Lurie, Galina; Eccles, Diana; Hein, Alexander; Beckmann, Matthias W; Ekici, Arif B; Paul, James; Brown, Robert; Flanagan, James M; Harter, Philipp; du Bois, Andreas; Schwaab, Ira; Hogdall, Claus K; Lundvall, Lene; Olson, Sara H; Orlow, Irene; Paddock, Lisa E; Rudolph, Anja; Eilber, Ursula; Dansonka-Mieszkowska, Agnieszka; Rzepecka, Iwona K; Ziolkowska-Seta, Izabela; Brinton, Louise; Yang, Hannah; Garcia-Closas, Montserrat; Despierre, Evelyn; Lambrechts, Sandrina; Vergote, Ignace; Walsh, Christine; Lester, Jenny; Sieh, Weiva; McGuire, Valerie; Rothstein, Joseph H; Ziogas, Argyrios; Lubinski, Jan; Cybulski, Cezary; Menkiszak, Janusz; Jensen, Allan; Gayther, Simon A; Ramus, Susan J; Gentry-Maharaj, Aleksandra; Berchuck, Andrew; Wu, Anna H; Pike, Malcolm C; Van Denberg, David; Terry, Kathryn L; Vitonis, Allison F; Doherty, Jennifer A; Johnatty, Sharon E; Defazio, Anna; Song, Honglin; Tyrer, Jonathan; Sellers, Thomas A; Phelan, Catherine M; Kalli, Kimberly R; Cunningham, Julie M; Fridley, Brooke L; Goode, Ellen L

    2013-05-01

    Ovarian cancer is a leading cause of cancer-related death among women. In an effort to understand contributors to disease outcome, we evaluated single-nucleotide polymorphisms (SNP) previously associated with ovarian cancer recurrence or survival, specifically in angiogenesis, inflammation, mitosis, and drug disposition genes. Twenty-seven SNPs in VHL, HGF, IL18, PRKACB, ABCB1, CYP2C8, ERCC2, and ERCC1 previously associated with ovarian cancer outcome were genotyped in 10,084 invasive cases from 28 studies from the Ovarian Cancer Association Consortium with over 37,000-observed person-years and 4,478 deaths. Cox proportional hazards models were used to examine the association between candidate SNPs and ovarian cancer recurrence or survival with and without adjustment for key covariates. We observed no association between genotype and ovarian cancer recurrence or survival for any of the SNPs examined. These results refute prior associations between these SNPs and ovarian cancer outcome and underscore the importance of maximally powered genetic association studies. These variants should not be used in prognostic models. Alternate approaches to uncovering inherited prognostic factors, if they exist, are needed.

  12. [Use of coronary angioplasty in acute myocardial infarction without previous use of thrombolytic agents. Analysis of 201 patients].

    Science.gov (United States)

    Mattos, L A; Cano, M N; Maldonado, G; Feres, F; Pinto, I M; Tanajura, L F; Piegas, L S; Barbosa, M; Timerman, A; Paulista, P P

    1990-11-01

    To evaluate the experience with the use of primary PTCA in the set of acute myocardial infarction (AMI) without the previous administration of thrombolytic agents. Two hundred and one patients with AMI, aged 35 to 86 years (mean = 57.2). There were 83.5% men. Twenty patients were older than 70 years and 48.2% had an anterior AMI. PTCA was performed early in the AMI, with a delay no longer than 12 hours. In 95%, it was done solely to the infarct related artery. Seventy percent patients were in Killip class I, 21% in class II, and 8% in classes III and IV (more than 50% of this subset of patients were in cardiogenic shock). Primary success was achieved in 84.5%. In hospital mortality was 5.9%, and there were 7% reinfarctions. Among the 84 patients who underwent a late cinecoronariography study, there were 19% restenosis and 8.3% reocclusions. In the long term follow up 75% were asymptomatic. Repeat PTCA was required in 10.2% of the cases, late coronary surgery was performed on 10.8% of the patients, and post-hospital cardiac mortality was 8.8% during an average follow up of 28 months (range 2-72 months). Primary PTCA is a effective way of reperfusion in the AMI, with a high primary success, low complications rate and without any major contraindications.

  13. Transcriptomic analysis in a Drosophila model identifies previously implicated and novel pathways in the therapeutic mechanism in neuropsychiatric disorders

    Directory of Open Access Journals (Sweden)

    Priyanka eSingh

    2011-03-01

    Full Text Available We have taken advantage of a newly described Drosophila model to gain insights into the potential mechanism of antiepileptic drugs (AEDs, a group of drugs that are widely used in the treatment of several neurological and psychiatric conditions besides epilepsy. In the recently described Drosophila model that is inspired by pentylenetetrazole (PTZ induced kindling epileptogenesis in rodents, chronic PTZ treatment for seven days causes a decreased climbing speed and an altered CNS transcriptome, with the latter mimicking gene expression alterations reported in epileptogenesis. In the model, an increased climbing speed is further observed seven days after withdrawal from chronic PTZ. We used this post-PTZ withdrawal regime to identify potential AED mechanism. In this regime, treatment with each of the five AEDs tested, namely, ethosuximide (ETH, gabapentin (GBP, vigabatrin (VGB, sodium valproate (NaVP and levetiracetam (LEV, resulted in rescuing of the altered climbing behavior. The AEDs also normalized PTZ withdrawal induced transcriptomic perturbation in fly heads; whereas AED untreated flies showed a large number of up- and down-regulated genes which were enriched in several processes including gene expression and cell communication, the AED treated flies showed differential expression of only a small number of genes that did not enrich gene expression and cell communication processes. Gene expression and cell communication related upregulated genes in AED untreated flies overrepresented several pathways - spliceosome, RNA degradation, and ribosome in the former category, and inositol phosphate metabolism, phosphatidylinositol signaling, endocytosis and hedgehog signaling in the latter. Transcriptome remodeling effect of AEDs was overall confirmed by microarray clustering that clearly separated the profiles of AED treated and untreated flies. Besides being consistent with previously implicated pathways, our results provide evidence for a role of

  14. Analysis of guest binary mixtures by tert-butylcalix[6]arene using host memory of previously bound guests.

    Science.gov (United States)

    Safina, Goulnaz D; Ziganshin, Marat A; Gubaidullin, Aidar T; Gorbatchuk, Valery V

    2013-02-28

    A new principle of quantitative and qualitative analysis of binary organic mixtures is offered, which is based on an ability of calixarene receptor for specific polymorphic transitions related to the composition of the analyzed guest mixture. The ability of tert-butylcalix[6]arene to remember selectively some guests bound from headspace both of pure liquids and their binary mixtures is used. The image of guest mixture remains written in metastable polymorphs of host after partial or complete guest elimination from clathrates. The memory was read using differential scanning calorimetry as the enthalpy of exothermic polymorphic transition of host collapse. This enthalpy monotonously changes with the variation of guests' ratio in mixture, unlike the enthalpies of endothermic pseudopolymorphic transitions of guest release. So, the composition of volatile binary mixture can be estimated using only one receptor and only one its parameter even in absence of preferential binding from a binary mixture of guests. This is an example of a genuine molecular recognition.

  15. Analysis of revista anacem’s articles production and its bibliographic references. Period 2008-2012.

    OpenAIRE

    Nicolás Alcayaga-Urrea.; Alys Hernández O.

    2013-01-01

    INTRODUCTION: Bibliographic material is essential for the sustenance of a investigation, since it validates new knowledge. OBJECTIVE: Analysis of Revista ANACEM’s articles production and characteristics of its bibliography. METHODS: Cross-sectional study including the total number of references in the journal. Description of distribution by type of article, average number and rank of references, language, insularity index, self-citation rate, year, obsolescence index and ...

  16. Primary reference materials for chemical composition analysis - Proposal for an operational definition.

    Science.gov (United States)

    Hässelbarth, W

    1996-01-01

    A metrologically motivated concept of a "Primary reference material (PRM)" for chemical composition analysis is proposed. This concept turns out to be essentially equivalent to that of a "Certified reference material (CRM)", as described in the relevant ISO Guides. Since the majority of actually available CRMs are far from realizing the theoretical concept, it is proposed to re-name and moderately revise the theoretical concept and to give it a new try to be put into practice.

  17. [Standard NF EN ISO 15189: comparative analysis with GBEA and implementation of the new reference support].

    Science.gov (United States)

    Rogowski, Julien; Annaix, Véronique

    2010-01-01

    Within reflexions of the healthcare system in France, Ballereau's report published in 2008 suggests modifications of medical biology supports by quality. The new reference system will be ISO 15189 which provides requirements for competence and quality. It differs from GBEA (Guidelines to Good Execution of Analysis) on the requirement of a quality system management. We accomplished a comparative study of these two standards of reference to identify differences, therefore elements to be worked or supported for accreditation process.

  18. [International reference prices and cost minimization analysis for the regulation of medicine prices in Colombia].

    Science.gov (United States)

    Vacca, Caludia; Acosta, Angela; Rodriguez, Ivan

    2011-01-01

    To suggest a scheme of decision making on pricing for medicines that are part of Free Regulated Regime, a regulation way of the pharmaceutical pricing policy in Colombia. It includes two regulation tools: international reference prices and a cost minimization analysis methodology. Following the current pricing policy, international reference prices were built with data from five countries for selected medicines, which are under Free Regulated Regime. The cost minimization analysis methodology includes selection of those medicines under Free Regulated Regime with possible comparable medicines, selection of comparable medicines, and treatment costs evaluation. As a result of the estimate of International Reference Prices, four medicines showed in the domestic pharmaceutical market a bigger price than the Reference Price. A scheme of decision-making was design containing two possible regulation tools for medicines that are part of Free Regulated Regime: estimate of international reference prices and cost minimization analysis methodology. This diagram would be useful to assist the pricing regulation of Free Regulated Regime in Colombia. As present results shows, international reference prices make clear when domestic prices are higher than those of reference countries. In the current regulation of pharmaceutical prices in Colombia, the international reference price has been applied for four medicines. Would be suitable to extend this methodology to other medicines of high impact on the pharmaceutical expenditure, in particular those covered by public funding. The availability of primary sources about treatment costs in Colombia needs to be improved as a requirement to develop pharmaco-economic evidence. SISMED is an official database that represents an important primary source of medicines prices in Colombia. Nevertheless, having into account that SISMED represents an important advantage of transparency in medicines prices, it needs to be improved in quality and data

  19. Precise Determination of Silicon in Ceramic Reference Materials by Prompt Gamma Activation Analysis at JRR-3

    Directory of Open Access Journals (Sweden)

    Tsutomu Miura

    2016-04-01

    Full Text Available Prompt gamma activation analysis using a thermal neutron-guided beam at Japan Atomic Energy Agency JRR-3M was applied for the precise determination of Si in silicon nitride ceramic reference materials [Japan Ceramic Reference Material (JCRM R 003]. In this study, the standard addition method coupled with internal standard was used for the nondestructive determination of Si in the sample. Cadmium was used as internal standard to obtain the linear calibration curves and to compensate for the neutron beam variability. The analytical result of determining Si in JCRM R 003 silicon nitride fine powder ceramic reference materials using prompt gamma activation analysis was in good agreement with that obtained by classical gravimetric analysis. The relative expanded measurement uncertainty (k = 2 associated with the determined value was 2.4%.

  20. Thermal analysis of optical reference cavities for low sensitivity to environmental temperature fluctuations.

    Science.gov (United States)

    Dai, Xiaojiao; Jiang, Yanyi; Hang, Chao; Bi, Zhiyi; Ma, Longsheng

    2015-02-23

    The temperature stability of optical reference cavities is significant in state-of-the-art ultra-stable narrow-linewidth laser systems. In this paper, the thermal time constant and thermal sensitivity of reference cavities are analyzed when reference cavities respond to environmental perturbations via heat transfer of thermal conduction and thermal radiation separately. The analysis as well as simulation results indicate that a reference cavity enclosed in multiple layers of thermal shields with larger mass, higher thermal capacity and lower emissivity is found to have a larger thermal time constant and thus a smaller sensitivity to environmental temperature perturbations. The design of thermal shields for reference cavities may vary according to experimentally achievable temperature stability and the coefficient of thermal expansion of reference cavities. A temperature fluctuation-induced length instability of reference cavities as low as 6 × 10(-16) on a day timescale can be achieved if a two-layer thermal shield is inserted between a cavity with the coefficient of thermal expansion of 1 × 10(-10) /K and an outer vacuum chamber with temperature fluctuation amplitude of 1 mK and period of 24 hours.

  1. Inference on the Univariate Frailty Model: A Bayesian Reference Analysis Approach

    Science.gov (United States)

    Tomazella, Vera Lucia D.; Martins, Camila Bertini; Bernardo, Jose Miguel

    2008-11-01

    In this work we present an approach involving objective Bayesian reference analysis to the Frailty model with univariate survival time and sources of heterogeneity that are not captured by covariates. The derivation unconditional hazard and survival leads to the Lomax distribution, also known as the Pareto distribution of the second kind. This distribution has an important position in life testing to adjust data from business failures. Reference analysis, introduced by Bernardo (1979) produce a new solution for this problem. The results are illustrated with survival data analyzed in the literature and simulated data.

  2. No-reference analysis of decoded MPEG images for PSNR estimation and post-processing

    DEFF Research Database (Denmark)

    Forchhammer, Søren; Li, Huiying; Andersen, Jakob Dahl

    2011-01-01

    We propose no-reference analysis and processing of DCT (Discrete Cosine Transform) coded images based on estimation of selected MPEG parameters from the decoded video. The goal is to assess MPEG video quality and perform post-processing without access to neither the original stream nor the code...... stream. Solutions are presented for MPEG-2 video. A method to estimate the quantization parameters of DCT coded images and MPEG I-frames at the macro-block level is presented. The results of this analysis is used for deblocking and deringing artifact reduction and no-reference PSNR estimation without...

  3. Building a Reduced Reference Video Quality Metric with Very Low Overhead Using Multivariate Data Analysis

    Directory of Open Access Journals (Sweden)

    Tobias Oelbaum

    2008-10-01

    Full Text Available In this contribution a reduced reference video quality metric for AVC/H.264 is proposed that needs only a very low overhead (not more than two bytes per sequence. This reduced reference metric uses well established algorithms to measure objective features of the video such as 'blur' or 'blocking'. Those measurements are then combined into a single measurement for the overall video quality. The weights of the single features and the combination of those are determined using methods provided by multivariate data analysis. The proposed metric is verified using a data set of AVC/H.264 encoded videos and the corresponding results of a carefully designed and conducted subjective evaluation. Results show that the proposed reduced reference metric not only outperforms standard PSNR but also two well known full reference metrics.

  4. Applicability of microwave digestion for analysis of elements in certified reference material by ICP-MS

    International Nuclear Information System (INIS)

    Md Suhaimi Elias; Ab Khalik Wood; Awad Al-Zahrany; Mohd Suhaimi Hamzah; Shamsiah Abd Rahman

    2007-01-01

    Quality of an analysis is contingent by several factors such as analysis method, equipment performance, standard preparation, sample preparation, staff competency and environmental condition. One of other major factor that also contributes to the quality of analysis results is the sample pre-treatment. In this study, sample pretreatment is involving digestion process of certified reference material using microwave oven whilst elemental analysis (Ba, Cd, Cu, Mo, Ni, Pb, and Ti) was done by Inductively Couple Plasma - Mass Spectrometer (ICP-MS). The applicability of the analytical method is confirmed by analysis results of the certified reference material (CRM). The results showed that the recovery of the elements of the CRM is 65% to 133% with Z-score value of -1.63 to 1.67. (Author)

  5. Validation Test For The Result Of Neutron Activation Analysis With Standard Reference Material

    International Nuclear Information System (INIS)

    Rina M, Th; Wardani, Sri

    2001-01-01

    The validity test with standard reference material is necessary to ensure the result analysis of samples. The analysis of CRM No.8 (Vehicle Exhaust Particulates) and SRM 1646a (Estuary Sediment) has been done in P2TRR. These analysis is intended to validate the analysis result done in NAA laboratory of P2TRR. For the CRM No. 8, is elements of the 30 certified elements were successfully analyzed Meanwhile, for SRM 1646a, 21 elements of the 39 certified elements were completely investigated The quantitative analysis showed the relative difference of 2% - 15% compared to the certificate

  6. Thyroid Function Within the Reference Range and the Risk of Stroke: An Individual Participant Data Analysis

    Science.gov (United States)

    Chaker, Layal; Baumgartner, Christine; den Elzen, Wendy P. J.; Collet, Tinh-Hai; Ikram, M. Arfan; Blum, Manuel R.; Dehghan, Abbas; Drechsler, Christiane; Luben, Robert N.; Portegies, Marileen L. P.; Iervasi, Giorgio; Medici, Marco; Stott, David J.; Dullaart, Robin P.; Ford, Ian; Bremner, Alexandra; Newman, Anne B.; Wanner, Christoph; Sgarbi, José A.; Dörr, Marcus; Longstreth, W. T.; Psaty, Bruce M.; Ferrucci, Luigi; Maciel, Rui M. B.; Westendorp, Rudi G.; Jukema, J. Wouter; Ceresini, Graziano; Imaizumi, Misa; Hofman, Albert; Bakker, Stephan J. L.; Franklyn, Jayne A.; Khaw, Kay-Tee; Bauer, Douglas C.; Walsh, John P.; Razvi, Salman; Gussekloo, Jacobijn; Völzke, Henry; Franco, Oscar H.; Cappola, Anne R.; Rodondi, Nicolas

    2016-01-01

    Context: The currently applied reference ranges for thyroid function are under debate. Despite evidence that thyroid function within the reference range is related with several cardiovascular disorders, its association with the risk of stroke has not been evaluated previously. Design and Setting: We identified studies through a systematic literature search and the Thyroid Studies Collaboration, a collaboration of prospective cohort studies. Studies measuring baseline TSH, free T4, and stroke outcomes were included, and we collected individual participant data from each study, including thyroid function measurements and incident all stroke (combined fatal and nonfatal) and fatal stroke. The applied reference range for TSH levels was between 0.45 and 4.49 mIU/L. Results: We collected individual participant data on 43 598 adults with TSH within the reference range from 17 cohorts, with a median follow-up of 11.6 years (interquartile range 5.1–13.9), including 449 908 person-years. Age- and sex-adjusted pooled hazard ratio for TSH was 0.78 (95% confidence interval [CI] 0.65–0.95 across the reference range of TSH) for all stroke and 0.83 (95% CI 0.62–1.09) for fatal stroke. For the free T4 analyses, the hazard ratio was 1.08 (95% CI 0.99–1.15 per SD increase) for all stroke and 1.10 (95% CI 1.04–1.19) for fatal stroke. This was independent of cardiovascular risk factors including systolic blood pressure, total cholesterol, smoking, and prevalent diabetes. Conclusion: Higher levels of TSH within the reference range may decrease the risk of stroke, highlighting the need for further research focusing on the clinical consequences associated with differences within the reference range of thyroid function. PMID:27603906

  7. Deep RNA sequencing analysis of readthrough gene fusions in human prostate adenocarcinoma and reference samples

    Directory of Open Access Journals (Sweden)

    Nacu Serban

    2011-01-01

    samples and 9 lung cancer cell lines. Conclusions Deep transcriptional sequencing and analysis with targeted and spliced alignment methods can effectively identify TIC events across the genome in individual tissues. Prostate and reference samples exhibit a wide range of TIC events, involving more genes than estimated previously using ESTs. Tissue specificity of TIC events is correlated with expression patterns of the upstream gene. Some TIC events, such as MSMB-NCOA4, may play functional roles in cancer.

  8. Retrospective analysis of patients self-referred to comprehensive ophthalmology seeking second opinions

    Directory of Open Access Journals (Sweden)

    Gologorsky D

    2013-06-01

    Full Text Available Daniel Gologorsky1, Scott H Greenstein2 1Dartmouth Medical School, Hanover, NH, USA; 2Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, USA Abstract: Patients choose to seek a second opinion in matters related to their health for a variety of reasons, and the total cost associated with these second opinion visits is estimated to be billions of dollars annually. Understanding the reasons behind second opinion self-referrals is key to improving patient satisfaction and reducing redundancy in delivered health care. This study represents a retrospective analysis of the records from a single provider at the Massachusetts Eye and Ear Infirmary (MEEI Comprehensive Ophthalmology Service in order to determine the various reasons that patients self-refer to an ophthalmology clinic seeking second opinions. A total of 174 patients presenting for a second opinion were identified over a one-year period. Patients presented for second opinions for two primary reasons: 60% presented in order to seek a confirmation of a diagnosis from an outside ophthalmologist (54% or optometrist (6%, and 40% presented due to a previous adverse experience with an outside provider, such as perceived treatment failure (26%, poor bedside manner (3%, distrust of the provider (5%, and poor provider communication skills (7%. This study strives to reiterate that the reduction of adverse patient experiences through effective communication of expected treatment options and outcomes, with a realistic time course of therapy, could significantly improve patient satisfaction and reduce costly second opinion visits. Keywords: referral, self-referral, second opinion, comprehensive ophthalmology

  9. SPARTA: Simple Program for Automated reference-based bacterial RNA-seq Transcriptome Analysis.

    Science.gov (United States)

    Johnson, Benjamin K; Scholz, Matthew B; Teal, Tracy K; Abramovitch, Robert B

    2016-02-04

    Many tools exist in the analysis of bacterial RNA sequencing (RNA-seq) transcriptional profiling experiments to identify differentially expressed genes between experimental conditions. Generally, the workflow includes quality control of reads, mapping to a reference, counting transcript abundance, and statistical tests for differentially expressed genes. In spite of the numerous tools developed for each component of an RNA-seq analysis workflow, easy-to-use bacterially oriented workflow applications to combine multiple tools and automate the process are lacking. With many tools to choose from for each step, the task of identifying a specific tool, adapting the input/output options to the specific use-case, and integrating the tools into a coherent analysis pipeline is not a trivial endeavor, particularly for microbiologists with limited bioinformatics experience. To make bacterial RNA-seq data analysis more accessible, we developed a Simple Program for Automated reference-based bacterial RNA-seq Transcriptome Analysis (SPARTA). SPARTA is a reference-based bacterial RNA-seq analysis workflow application for single-end Illumina reads. SPARTA is turnkey software that simplifies the process of analyzing RNA-seq data sets, making bacterial RNA-seq analysis a routine process that can be undertaken on a personal computer or in the classroom. The easy-to-install, complete workflow processes whole transcriptome shotgun sequencing data files by trimming reads and removing adapters, mapping reads to a reference, counting gene features, calculating differential gene expression, and, importantly, checking for potential batch effects within the data set. SPARTA outputs quality analysis reports, gene feature counts and differential gene expression tables and scatterplots. SPARTA provides an easy-to-use bacterial RNA-seq transcriptional profiling workflow to identify differentially expressed genes between experimental conditions. This software will enable microbiologists with

  10. Preoperative screening: value of previous tests.

    Science.gov (United States)

    Macpherson, D S; Snow, R; Lofgren, R P

    1990-12-15

    To determine the frequency of tests done in the year before elective surgery that might substitute for preoperative screening tests and to determine the frequency of test results that change from a normal value to a value likely to alter perioperative management. Retrospective cohort analysis of computerized laboratory data (complete blood count, sodium, potassium, and creatinine levels, prothrombin time, and partial thromboplastin time). Urban tertiary care Veterans Affairs Hospital. Consecutive sample of 1109 patients who had elective surgery in 1988. At admission, 7549 preoperative tests were done, 47% of which duplicated tests performed in the previous year. Of 3096 previous results that were normal as defined by hospital reference range and done closest to the time of but before admission (median interval, 2 months), 13 (0.4%; 95% CI, 0.2% to 0.7%), repeat values were outside a range considered acceptable for surgery. Most of the abnormalities were predictable from the patient's history, and most were not noted in the medical record. Of 461 previous tests that were abnormal, 78 (17%; CI, 13% to 20%) repeat values at admission were outside a range considered acceptable for surgery (P less than 0.001, frequency of clinically important abnormalities of patients with normal previous results with those with abnormal previous results). Physicians evaluating patients preoperatively could safely substitute the previous test results analyzed in this study for preoperative screening tests if the previous tests are normal and no obvious indication for retesting is present.

  11. Quantitative analysis of [{sup 18}F]FDDNP PET using subcortical white matter as reference region

    Energy Technology Data Exchange (ETDEWEB)

    Wong, Koon-Pong; Shao, Weber; Dahlbom, Magnus; Kepe, Vladimir; Liu, Jie; Satyamurthy, Nagichettiar; Barrio, Jorge R. [David Geffen School of Medicine at UCLA, Department of Molecular and Medical Pharmacology, Los Angeles, CA (United States); Wardak, Mirwais; Huang, Sung-Cheng [David Geffen School of Medicine at UCLA, Department of Molecular and Medical Pharmacology, Los Angeles, CA (United States); David Geffen School of Medicine at UCLA, Department of Biomathematics, Los Angeles, CA (United States); Small, Gary W. [David Geffen School of Medicine at UCLA, Department of Psychiatry and Biobehavioral Sciences, Los Angeles, CA (United States); David Geffen School of Medicine at UCLA, Semel Institute for Neuroscience and Human Behavior, Los Angeles, CA (United States); David Geffen School of Medicine at UCLA, UCLA Center on Aging, Los Angeles, CA (United States); Mary S. Easton Center for Alzheimer' s Disease Research, Los Angeles, CA (United States)

    2010-03-15

    Subcortical white matter is known to be relatively unaffected by amyloid deposition in Alzheimer's disease (AD). We investigated the use of subcortical white matter as a reference region to quantify [{sup 18}F]FDDNP binding in the human brain. Dynamic [{sup 18}F]FDDNP PET studies were performed on 7 control subjects and 12 AD patients. Population efflux rate constants (k{sup '}{sub 2}) from subcortical white matter (centrum semiovale) and cerebellar cortex were derived by a simplified reference tissue modeling approach incorporating physiological constraints. Regional distribution volume ratio (DVR) estimates were derived using Logan and simplified reference tissue approaches, with either subcortical white matter or cerebellum as reference input. Discriminant analysis with cross-validation was performed to classify control subjects and AD patients. The population estimates of k{sup '}{sub 2} in subcortical white matter did not differ significantly between control subjects and AD patients but the variability of individual estimates of k{sup '}{sub 2} determined in white matter was lower than that in cerebellum. Logan DVR showed dependence on the efflux rate constant in white matter. The DVR estimates in the frontal, parietal, posterior cingulate, and temporal cortices were significantly higher in the AD group (p<0.01). Incorporating all these regional DVR estimates as predictor variables in discriminant analysis yielded accurate classification of control subjects and AD patients with high sensitivity and specificity, and the results agreed well with those using the cerebellum as the reference region. Subcortical white matter can be used as a reference region for quantitative analysis of [{sup 18}F]FDDNP with the Logan method which allows more accurate and less biased binding estimates, but a population efflux rate constant has to be determined a priori. (orig.)

  12. Principal component analysis of reference sites used for calibration and validation of Earth observation satellites

    Science.gov (United States)

    Campos, Joaquin; Ferrero, Alejandro; Woolliams, Emma; Greenwell, Claire; Bialek, Agnieszka; Hernanz, Luisa; Pons, Alicia

    2018-02-01

    Determining reflectance factor and its variability across reference sites for Earth observation satellites is a problem involving large amounts of data and measurement time. Principal component analysis (PCA) may be used to simplify this problem by reducing the size of the data and by highlighting spectral features that could be related to physical phenomena. This work presents the results obtained in applying PCA to two reference sites for calibration and validation of Earth observation satellites located at La Crau (France) and Gobabeb (Namibia), respectively.

  13. [Investigation of reference intervals of blood gas and acid-base analysis assays in China].

    Science.gov (United States)

    Zhang, Lu; Wang, Wei; Wang, Zhiguo

    2015-10-01

    To investigate and analyze the upper and lower limits and their sources of reference intervals in blood gas and acid-base analysis assays. The data of reference intervals were collected, which come from the first run of 2014 External Quality Assessment (EQA) program in blood gas and acid-base analysis assays performed by National Center for Clinical Laboratories (NCCL). All the abnormal values and errors were eliminated. Data statistics was performed by SPSS 13.0 and Excel 2007 referring to upper and lower limits of reference intervals and sources of 7 blood gas and acid-base analysis assays, i.e. pH value, partial pressure of carbon dioxide (PCO2), partial pressure of oxygen (PO2), Na+, K+, Ca2+ and Cl-. Values were further grouped based on instrument system and the difference between each group were analyzed. There were 225 laboratories submitting the information on the reference intervals they had been using. The three main sources of reference intervals were National Guide to Clinical Laboratory Procedures [37.07% (400/1 079)], instructions of instrument manufactures [31.23% (337/1 079)] and instructions of reagent manufactures [23.26% (251/1 079)]. Approximately 35.1% (79/225) of the laboratories had validated the reference intervals they used. The difference of upper and lower limits in most assays among 7 laboratories was moderate, both minimum and maximum (i.e. the upper limits of pH value was 7.00-7.45, the lower limits of Na+ was 130.00-156.00 mmol/L), and mean and median (i.e. the upper limits of K+ was 5.04 mmol/L and 5.10 mmol/L, the upper limits of PCO2 was 45.65 mmHg and 45.00 mmHg, 1 mmHg = 0.133 kPa), as well as the difference in P2.5 and P97.5 between each instrument system group. It was shown by Kruskal-Wallis method that the P values of upper and lower limits of all the parameters were lower than 0.001, expecting the lower limits of Na+ with P value 0.029. It was shown by Mann-Whitney that the statistic differences were found among instrument

  14. SPM analysis of parametric (R)-[11C]PK11195 binding images: plasma input versus reference tissue parametric methods.

    Science.gov (United States)

    Schuitemaker, Alie; van Berckel, Bart N M; Kropholler, Marc A; Veltman, Dick J; Scheltens, Philip; Jonker, Cees; Lammertsma, Adriaan A; Boellaard, Ronald

    2007-05-01

    (R)-[11C]PK11195 has been used for quantifying cerebral microglial activation in vivo. In previous studies, both plasma input and reference tissue methods have been used, usually in combination with a region of interest (ROI) approach. Definition of ROIs, however, can be labourious and prone to interobserver variation. In addition, results are only obtained for predefined areas and (unexpected) signals in undefined areas may be missed. On the other hand, standard pharmacokinetic models are too sensitive to noise to calculate (R)-[11C]PK11195 binding on a voxel-by-voxel basis. Linearised versions of both plasma input and reference tissue models have been described, and these are more suitable for parametric imaging. The purpose of this study was to compare the performance of these plasma input and reference tissue parametric methods on the outcome of statistical parametric mapping (SPM) analysis of (R)-[11C]PK11195 binding. Dynamic (R)-[11C]PK11195 PET scans with arterial blood sampling were performed in 7 younger and 11 elderly healthy subjects. Parametric images of volume of distribution (Vd) and binding potential (BP) were generated using linearised versions of plasma input (Logan) and reference tissue (Reference Parametric Mapping) models. Images were compared at the group level using SPM with a two-sample t-test per voxel, both with and without proportional scaling. Parametric BP images without scaling provided the most sensitive framework for determining differences in (R)-[11C]PK11195 binding between younger and elderly subjects. Vd images could only demonstrate differences in (R)-[11C]PK11195 binding when analysed with proportional scaling due to intersubject variation in K1/k2 (blood-brain barrier transport and non-specific binding).

  15. Retrospective Analysis of NIST Standard Reference Material 1450, Fibrous Glass Board, for Thermal Insulation Measurements

    Science.gov (United States)

    Zarr, Robert R; Heckert, N Alan; Leigh, Stefan D

    2014-01-01

    Thermal conductivity data acquired previously for the establishment of Standard Reference Material (SRM) 1450, Fibrous Glass Board, as well as subsequent renewals 1450a, 1450b, 1450c, and 1450d, are re-analyzed collectively and as individual data sets. Additional data sets for proto-1450 material lots are also included in the analysis. The data cover 36 years of activity by the National Institute of Standards and Technology (NIST) in developing and providing thermal insulation SRMs, specifically high-density molded fibrous-glass board, to the public. Collectively, the data sets cover two nominal thicknesses of 13 mm and 25 mm, bulk densities from 60 kg·m−3 to 180 kg·m−3, and mean temperatures from 100 K to 340 K. The analysis repetitively fits six models to the individual data sets. The most general form of the nested set of multilinear models used is given in the following equation: λ(ρ,T)=a0+a1ρ+a2T+a3T3+a4e−(T−a5a6)2where λ(ρ,T) is the predicted thermal conductivity (W·m−1·K−1), ρ is the bulk density (kg·m−3), T is the mean temperature (K) and ai (for i = 1, 2, … 6) are the regression coefficients. The least squares fit results for each model across all data sets are analyzed using both graphical and analytic techniques. The prevailing generic model for the majority of data sets is the bilinear model in ρ and T. λ(ρ,T)=a0+a1ρ+a2T One data set supports the inclusion of a cubic temperature term and two data sets with low-temperature data support the inclusion of an exponential term in T to improve the model predictions. Physical interpretations of the model function terms are described. Recommendations for future renewals of SRM 1450 are provided. An Addendum provides historical background on the origin of this SRM and the influence of the SRM on external measurement programs. PMID:26601034

  16. Dosimetric analysis at ICRU reference points in HDR-brachytherapy of cervical carcinoma.

    Science.gov (United States)

    Eich, H T; Haverkamp, U; Micke, O; Prott, F J; Müller, R P

    2000-01-01

    In vivo dosimetry in bladder and rectum as well as determining doses on suggested reference points following the ICRU report 38 contribute to quality assurance in HDR-brachytherapy of cervical carcinoma, especially to minimize side effects. In order to gain information regarding the radiation exposure at ICRU reference points in rectum, bladder, ureter and regional lymph nodes those were calculated (digitalisation) by means of orthogonal radiographs of 11 applications in patients with cervical carcinoma, who received primary radiotherapy. In addition, the doses at the ICRU rectum reference point was compared to the results of in vivo measurements in the rectum. The in vivo measurements were by factor 1.5 below the doses determined for the ICRU rectum reference point (4.05 +/- 0.68 Gy versus 6.11 +/- 1.63 Gy). Reasons for this were: calibration errors, non-orthogonal radiographs, movement of applicator and probe in the time span between X-ray and application, missing connection of probe and anterior rectal wall. The standard deviation of calculations at ICRU reference points was on average +/- 30%. Possible reasons for the relatively large standard deviation were difficulties in defining the points, identifying them on radiographs and the different locations of the applicators. Although 3 D CT, US or MR based treatment planning using dose volume histogram analysis is more and more established, this simple procedure of marking and digitising the ICRU reference points lengthened treatment planning only by 5 to 10 minutes. The advantages of in vivo dosimetry are easy practicability and the possibility to determine rectum doses during radiation. The advantages of computer-aided planning at ICRU reference points are that calculations are available before radiation and that they can still be taken into account for treatment planning. Both methods should be applied in HDR-brachytherapy of cervical carcinoma.

  17. Selection and validation of reference genes for gene expression analysis in apomictic and sexual Cenchrus ciliaris

    Science.gov (United States)

    2013-01-01

    Background Apomixis is a naturally occurring asexual mode of seed reproduction resulting in offspring genetically identical to the maternal plant. Identifying differential gene expression patterns between apomictic and sexual plants is valuable to help deconstruct the trait. Quantitative RT-PCR (qRT-PCR) is a popular method for analyzing gene expression. Normalizing gene expression data using proper reference genes which show stable expression under investigated conditions is critical in qRT-PCR analysis. We used qRT-PCR to validate expression and stability of six potential reference genes (EF1alpha, EIF4A, UBCE, GAPDH, ACT2 and TUBA) in vegetative and reproductive tissues of B-2S and B-12-9 accessions of C. ciliaris. Findings Among tissue types evaluated, EF1alpha showed the highest level of expression while TUBA showed the lowest. When all tissue types were evaluated and compared between genotypes, EIF4A was the most stable reference gene. Gene expression stability for specific ovary stages of B-2S and B-12-9 was also determined. Except for TUBA, all other tested reference genes could be used for any stage-specific ovary tissue normalization, irrespective of the mode of reproduction. Conclusion Our gene expression stability assay using six reference genes, in sexual and apomictic accessions of C. ciliaris, suggests that EIF4A is the most stable gene across all tissue types analyzed. All other tested reference genes, with the exception of TUBA, could be used for gene expression comparison studies between sexual and apomictic ovaries over multiple developmental stages. This reference gene validation data in C. ciliaris will serve as an important base for future apomixis-related transcriptome data validation. PMID:24083672

  18. Evaluation of reference genes for gene expression analysis using quantitative RT-PCR in Azospirillum brasilense.

    Directory of Open Access Journals (Sweden)

    Mary McMillan

    Full Text Available Azospirillum brasilense is a nitrogen fixing bacterium that has been shown to have various beneficial effects on plant growth and yield. Under normal conditions A. brasilense exists in a motile flagellated form, which, under starvation or stress conditions, can undergo differentiation into an encapsulated, cyst-like form. Quantitative RT-PCR can be used to analyse changes in gene expression during this differentiation process. The accuracy of quantification of mRNA levels by qRT-PCR relies on the normalisation of data against stably expressed reference genes. No suitable set of reference genes has yet been described for A. brasilense. Here we evaluated the expression of ten candidate reference genes (16S rRNA, gapB, glyA, gyrA, proC, pykA, recA, recF, rpoD, and tpiA in wild-type and mutant A. brasilense strains under different culture conditions, including conditions that induce differentiation. Analysis with the software programs BestKeeper, NormFinder and GeNorm indicated that gyrA, glyA and recA are the most stably expressed reference genes in A. brasilense. The results also suggested that the use of two reference genes (gyrA and glyA is sufficient for effective normalisation of qRT-PCR data.

  19. Evaluation of reference genes for gene expression analysis using quantitative RT-PCR in Azospirillum brasilense.

    Science.gov (United States)

    McMillan, Mary; Pereg, Lily

    2014-01-01

    Azospirillum brasilense is a nitrogen fixing bacterium that has been shown to have various beneficial effects on plant growth and yield. Under normal conditions A. brasilense exists in a motile flagellated form, which, under starvation or stress conditions, can undergo differentiation into an encapsulated, cyst-like form. Quantitative RT-PCR can be used to analyse changes in gene expression during this differentiation process. The accuracy of quantification of mRNA levels by qRT-PCR relies on the normalisation of data against stably expressed reference genes. No suitable set of reference genes has yet been described for A. brasilense. Here we evaluated the expression of ten candidate reference genes (16S rRNA, gapB, glyA, gyrA, proC, pykA, recA, recF, rpoD, and tpiA) in wild-type and mutant A. brasilense strains under different culture conditions, including conditions that induce differentiation. Analysis with the software programs BestKeeper, NormFinder and GeNorm indicated that gyrA, glyA and recA are the most stably expressed reference genes in A. brasilense. The results also suggested that the use of two reference genes (gyrA and glyA) is sufficient for effective normalisation of qRT-PCR data.

  20. Transaction Analysis of Interactions at the Reference Desk of a Small Academic Library

    Directory of Open Access Journals (Sweden)

    Heather Empey

    2011-01-01

    Full Text Available As discussions continue about the changing nature of reference service in academic libraries, the Geoffrey R. Weller Library determined that more detailed information on what was happening at the Reference Desk was needed. During the 2006/07 academic year, transactions at the Reference Desk were analyzed to determine when they occurred (both during the week and during the academic year, the length of time the transactions took, the categories of the transactions, what sources were used and whether or not instruction was provided as part of the transaction. Another round of data was gathered in September 2009 to determine if use patterns had changed. Transactions at the Reference Desk were generally conducted in person, took either <1 min. or between 1-5 min. to answer, and occurred most often on Mon-Thurs between 11am-5pm. Between September 2006 and September 2009, specific title and research categories of questions decreased by 6% and directional and technical help categories of questions increased by 9%. There was also a decrease in the level of instruction being given. As a result of this research, service hours have been reduced and the on-going data collection at the Reference Desk has become more detailed to allow for ongoing analysis.

  1. Consistent Feature Extraction From Vector Fields: Combinatorial Representations and Analysis Under Local Reference Frames

    Energy Technology Data Exchange (ETDEWEB)

    Bhatia, Harsh [Univ. of Utah, Salt Lake City, UT (United States)

    2015-05-01

    This dissertation presents research on addressing some of the contemporary challenges in the analysis of vector fields—an important type of scientific data useful for representing a multitude of physical phenomena, such as wind flow and ocean currents. In particular, new theories and computational frameworks to enable consistent feature extraction from vector fields are presented. One of the most fundamental challenges in the analysis of vector fields is that their features are defined with respect to reference frames. Unfortunately, there is no single “correct” reference frame for analysis, and an unsuitable frame may cause features of interest to remain undetected, thus creating serious physical consequences. This work develops new reference frames that enable extraction of localized features that other techniques and frames fail to detect. As a result, these reference frames objectify the notion of “correctness” of features for certain goals by revealing the phenomena of importance from the underlying data. An important consequence of using these local frames is that the analysis of unsteady (time-varying) vector fields can be reduced to the analysis of sequences of steady (timeindependent) vector fields, which can be performed using simpler and scalable techniques that allow better data management by accessing the data on a per-time-step basis. Nevertheless, the state-of-the-art analysis of steady vector fields is not robust, as most techniques are numerical in nature. The residing numerical errors can violate consistency with the underlying theory by breaching important fundamental laws, which may lead to serious physical consequences. This dissertation considers consistency as the most fundamental characteristic of computational analysis that must always be preserved, and presents a new discrete theory that uses combinatorial representations and algorithms to provide consistency guarantees during vector field analysis along with the uncertainty

  2. To what do psychiatric diagnoses refer? A two-dimensional semantic analysis of diagnostic terms

    Science.gov (United States)

    Maung, Hane Htut

    2016-01-01

    In somatic medicine, diagnostic terms often refer to the disease processes that are the causes of patients' symptoms. The language used in some clinical textbooks and health information resources suggests that this is also sometimes assumed to be the case with diagnoses in psychiatry. However, this seems to be in tension with the ways in which psychiatric diagnoses are defined in diagnostic manuals, according to which they refer solely to clusters of symptoms. This paper explores how theories of reference in the philosophy of language can help to resolve this tension. After the evaluation of descriptive and causal theories of reference, I put forward a conceptual framework based on two-dimensional semantics that allows the causal analysis of diagnostic terms in psychiatry, while taking seriously their descriptive definitions in diagnostic manuals. While the framework is presented as a solution to a problem regarding the semantics of psychiatric diagnoses, it can also accommodate the analysis of diagnostic terms in other medical disciplines. PMID:26580354

  3. Probabilistic sensitivity analysis for the 'initial defect in the canister' reference model

    International Nuclear Information System (INIS)

    Cormenzana, J. L.

    2013-08-01

    In Posiva Oy's Safety Case 'TURVA-2012' the repository system scenarios leading to radionuclide releases have been identified in Formulation of Radionuclide Release Scenarios. Three potential causes of canister failure and radionuclide release are considered: (i) the presence of an initial defect in the copper shell of one canister that penetrates the shell completely, (ii) corrosion of the copper overpack, that occurs more rapidly if buffer density is reduced, e.g. by erosion, (iii) shear movement on fractures intersecting the deposition hole. All three failure modes are analysed deterministically in Assessment of Radionuclide Release Scenarios, and for the 'initial defect in the canister' reference model a probabilistic sensitivity analysis (PSA) has been carried out. The main steps of the PSA have been: quantification of the uncertainties in the model input parameters through the creation of probability density distributions (PDFs), Monte Carlo simulations of the evolution of the system up to 106 years using parameters values sampled from the previous PDFs. Monte Carlo simulations with 10,000 individual calculations (realisations) have been used in the PSA, quantification of the uncertainty in the model outputs due to uncertainty in the input parameters (uncertainty analysis), and identification of the parameters whose uncertainty have the greatest effect on the uncertainty in the model outputs (sensitivity analysis) Since the biosphere is not included in the Monte Carlo simulations of the system, the model outputs studied are not doses, but total and radionuclide-specific normalised release rates from the near-field and to the biosphere. These outputs are calculated dividing the activity release rates by the constraints on the activity fluxes to the environment set out by the Finnish regulator. Two different cases are analysed in the PSA: (i) the 'hole forever' case, in which the small hole through the copper overpack remains unchanged during the assessment

  4. Multivariate methods for analysis of environmental reference materials using laser-induced breakdown spectroscopy

    Directory of Open Access Journals (Sweden)

    Shikha Awasthi

    2017-06-01

    Full Text Available Analysis of emission from laser-induced plasma has a unique capability for quantifying the major and minor elements present in any type of samples under optimal analysis conditions. Chemometric techniques are very effective and reliable tools for quantification of multiple components in complex matrices. The feasibility of laser-induced breakdown spectroscopy (LIBS in combination with multivariate analysis was investigated for the analysis of environmental reference materials (RMs. In the present work, different (Certified/Standard Reference Materials of soil and plant origin were analyzed using LIBS and the presence of Al, Ca, Mg, Fe, K, Mn and Si were identified in the LIBS spectra of these materials. Multivariate statistical methods (Partial Least Square Regression and Partial Least Square Discriminant Analysis were employed for quantitative analysis of the constituent elements using the LIBS spectral data. Calibration models were used to predict the concentrations of the different elements of test samples and subsequently, the concentrations were compared with certified concentrations to check the authenticity of models. The non-destructive analytical method namely Instrumental Neutron Activation Analysis (INAA using high flux reactor neutrons and high resolution gamma-ray spectrometry was also used for intercomparison of results of two RMs by LIBS.

  5. A meta-analysis of drug resistant tuberculosis in Sub-Saharan Africa: how strongly associated with previous treatment and HIV co-infection?

    Science.gov (United States)

    Berhan, Asres; Berhan, Yifru; Yizengaw, Desalegn

    2013-11-01

    In Sub-Saharan Africa, the fight against tuberculosis (TB) has encountered a great challenge because of the emergence of drug resistant TB strains and the high prevalence of HIV infection. The aim of this meta-analysis was to determine the association of drug-resistant TB with anti-TB drug treatment history and HIV co-infection. After electronic based literature search in the databases of Medline, HINARI, EMBASE and the Cochrane library, article selection and data extraction were carried out. HIV co-infection and previous history of TB treatment were used as predictors for the occurrence of any anti-TB drug resistant or multiple drug resistant TB (MDR-TB). The risk ratios for each included study and for the pooled sample were computed using the random-effects model. Heterogeneity test, sensitivity analyses and funnel plots were also done. The pooled analysis showed that the risk of developing drug-resistant TB to at least one anti-TB drug was about 3 times higher in individuals who had a previous history of anti-TB treatment than new TB cases. The risk of having MDR-TB in previously anti-TB treated TB cases was more than 5-fold higher than that of new TB cases. Resistance to Ethambutol and Rifampicin was more than fivefold higher among the previously treated with anti-TB drugs. However, HIV infection was not associated with drug-resistant TB. There was a strong association of previous anti-TB treatment with MDR-TB. Primary treatment warrants special emphasis, and screening for anti-TB drugs sensitivity has to be strengthened.

  6. PREVIOUS SECOND TRIMESTER ABORTION

    African Journals Online (AJOL)

    PNLC

    PREVIOUS SECOND TRIMESTER ABORTION: A risk factor for third trimester uterine rupture in three ... for accurate diagnosis of uterine rupture. KEY WORDS: Induced second trimester abortion - Previous uterine surgery - Uterine rupture. ..... scarred uterus during second trimester misoprostol- induced labour for a missed ...

  7. Homogeneity study on biological candidate reference materials: the role of neutron activation analysis

    International Nuclear Information System (INIS)

    Silva, Daniel P.; Moreira, Edson G.

    2015-01-01

    Instrumental Neutron activation Analysis (INAA) is a mature nuclear analytical technique able to accurately determine chemical elements without the need of sample digestion and, hence, without the associated problems of analyte loss or contamination. This feature, along with its potentiality use as a primary method of analysis, makes it an important tool for the characterization of new references materials and in the assessment of their homogeneity status. In this study, the ability of the comparative method of INAA for the within-bottle homogeneity of K, Mg, Mn and V in a mussel reference material was investigated. Method parameters, such as irradiation time, sample decay time and distance from sample to the detector were varied in order to allow element determination in subsamples of different sample masses in duplicate. Sample masses were in the range of 1 to 250 mg and the limitations of the detection limit for small sample masses and dead time distortions for large sample masses were investigated. (author)

  8. Washout allometric reference method (WARM) for parametric analysis of [(11)C]PIB in human brains

    DEFF Research Database (Denmark)

    Rodell, Anders; Aanerud, Joel; Braendgaard, Hans

    2013-01-01

    Rapid clearance and disappearance of a tracer from the circulation challenges the determination of the tracer's binding potentials in brain (BP ND) by positron emission tomography (PET). This is the case for the analysis of the binding of radiolabeled [(11)C]Pittsburgh Compound B ([(11)C]PIB......) to amyloid-β (Aβ) plaques in brain of patients with Alzheimer's disease (AD). To resolve the issue of rapid clearance from the circulation, we here introduce the flow-independent Washout Allometric Reference Method (WARM) for the analysis of washout and binding of [(11)C]PIB in two groups of human subjects...... reference region directly from the [(11)C]PIB signal. The difference of average absolute CBF values between the AD and HC groups was highly significant (P

  9. Homogeneity study on biological candidate reference materials: the role of neutron activation analysis

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Daniel P.; Moreira, Edson G., E-mail: dsilva.pereira@usp.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2015-07-01

    Instrumental Neutron activation Analysis (INAA) is a mature nuclear analytical technique able to accurately determine chemical elements without the need of sample digestion and, hence, without the associated problems of analyte loss or contamination. This feature, along with its potentiality use as a primary method of analysis, makes it an important tool for the characterization of new references materials and in the assessment of their homogeneity status. In this study, the ability of the comparative method of INAA for the within-bottle homogeneity of K, Mg, Mn and V in a mussel reference material was investigated. Method parameters, such as irradiation time, sample decay time and distance from sample to the detector were varied in order to allow element determination in subsamples of different sample masses in duplicate. Sample masses were in the range of 1 to 250 mg and the limitations of the detection limit for small sample masses and dead time distortions for large sample masses were investigated. (author)

  10. Third research coordination meeting on reference database for neutron activation analysis. Summary report

    International Nuclear Information System (INIS)

    Kellett, M.A.

    2009-12-01

    The third meeting of the Co-ordinated Research Project on 'Reference Database for Neutron Activation Analysis' was held at the IAEA, Vienna from 17-19 November 2008. A summary of presentations made by participants is given, reports on specific tasks and subsequent discussions. With the aim of finalising the work of this CRP and in order to meet initial objectives, outputs were discussed and detailed task assignments agreed upon. (author)

  11. Determination of Cd and Cr in an ABS candidate reference material by instrumental neutron activation analysis

    Energy Technology Data Exchange (ETDEWEB)

    Park, Kwangwon [Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, 1 Doryong-Dong, Yuseong-Gu, Daejeon 305-340 (Korea, Republic of)], E-mail: parkkw@kriss.re.kr; Kang, Namgoo; Cho, Kyunghaeng; Lee, Jounghae [Division of Metrology for Quality Life, Korea Research Institute of Standards and Science, 1 Doryong-Dong, Yuseong-Gu, Daejeon 305-340 (Korea, Republic of)

    2008-12-15

    In order to practically better cope with technical barriers to trade (TBT) of a great number of resin goods, our research presents first-ever results for the determination of Cd and Cr in acrylonitrile butadiene styrene (ABS) candidate reference material using instrumental neutron activation analysis (INAA) recently recognized as a candidate primary ratio method with a particular attention to the estimation of involved measurement uncertainties.

  12. Fit Gap Analysis – The Role of Business Process Reference Models

    Directory of Open Access Journals (Sweden)

    Dejan Pajk

    2013-12-01

    Full Text Available Enterprise resource planning (ERP systems support solutions for standard business processes such as financial, sales, procurement and warehouse. In order to improve the understandability and efficiency of their implementation, ERP vendors have introduced reference models that describe the processes and underlying structure of an ERP system. To select and successfully implement an ERP system, the capabilities of that system have to be compared with a company’s business needs. Based on a comparison, all of the fits and gaps must be identified and further analysed. This step usually forms part of ERP implementation methodologies and is called fit gap analysis. The paper theoretically overviews methods for applying reference models and describes fit gap analysis processes in detail. The paper’s first contribution is its presentation of a fit gap analysis using standard business process modelling notation. The second contribution is the demonstration of a process-based comparison approach between a supply chain process and an ERP system process reference model. In addition to its theoretical contributions, the results can also be practically applied to projects involving the selection and implementation of ERP systems.

  13. Validation of suitable reference genes for quantitative gene expression analysis in Panax ginseng

    Directory of Open Access Journals (Sweden)

    Meizhen eWang

    2016-01-01

    Full Text Available Reverse transcription-qPCR (RT-qPCR has become a popular method for gene expression studies. Its results require data normalization by housekeeping genes. No single gene is proved to be stably expressed under all experimental conditions. Therefore, systematic evaluation of reference genes is necessary. With the aim to identify optimum reference genes for RT-qPCR analysis of gene expression in different tissues of Panax ginseng and the seedlings grown under heat stress, we investigated the expression stability of eight candidate reference genes, including elongation factor 1-beta (EF1-β, elongation factor 1-gamma (EF1-γ, eukaryotic translation initiation factor 3G (IF3G, eukaryotic translation initiation factor 3B (IF3B, actin (ACT, actin11 (ACT11, glyceraldehyde-3-phosphate dehydrogenase (GAPDH and cyclophilin ABH-like protein (CYC, using four widely used computational programs: geNorm, Normfinder, BestKeeper, and the comparative ΔCt method. The results were then integrated using the web-based tool RefFinder. As a result, EF1-γ, IF3G and EF1-β were the three most stable genes in different tissues of P. ginseng, while IF3G, ACT11 and GAPDH were the top three-ranked genes in seedlings treated with heat. Using three better reference genes alone or in combination as internal control, we examined the expression profiles of MAR, a multiple function-associated mRNA-like non-coding RNA (mlncRNA in P. ginseng. Taken together, we recommended EF1-γ/IF3G and IF3G/ACT11 as the suitable pair of reference genes for RT-qPCR analysis of gene expression in different tissues of P. ginseng and the seedlings grown under heat stress, respectively. The results serve as a foundation for future studies on P. ginseng functional genomics.

  14. Polish reference material: corn flour (INCT-CF-3)for inorganic trace analysis - preparation and certification

    International Nuclear Information System (INIS)

    Polkowska-Motrenko, H.; Dybczynski, R.; Chajduk, E.; Danko, B.; Kulisa, K.; Samczynski, Z.; Sypula, M.; Szopa, Z.

    2006-01-01

    Preparation, examination and certification of the new matrix reference material of biological origin: Corn Flour (INCT-CF-3) is described. The material was prepared from corn grown in Poland according to Polish standard PN-A-74205:1997. The material was sieved through the 250 mm nylon sieves and stored in a polyethylene (PE) bag. Approximately 50 kg of sieved corn flour was collected. Examination by optical microscopy revealed that Martin's diameter of over 98% of particles was below 25 mm. The whole lot of corn flour was then homogenized by mixing for 20 hours in a 110 dm 3 PE drum rotated in three directions. Preliminary homogeneity testing by X-ray fluorescence (XRF) method and final checking of homogeneity by neutron activation analysis (NAA) after distribution of the material into containers revealed, that it is sufficiently homogeneous at least for a sample size ≥ 100 mg. In order to assure the long-term stability, all containers with INCT-CF-3 were sterilized by electron beam radiation. Long-term stability was checked by analyzing concentrations of selected elements in the material stored in the air-conditioned room at 20 o C. Short-term stability was examined by the determination of concentrations of the selected elements in the bottle stored in the CO 2 incubator at 37 o C. The material was certified on the basis of a worldwide interlaboratory comparison, in which 92 laboratories from 19 countries participated providing 962 laboratory averages (4228 individual determinations) for 57 elements. A method of data evaluation leading to assignment of certified values was the same as that used previously in the Laboratory of the Department of Analytical Chemistry, Institute of Nuclear Chemistry and Technology. The result for Mo was obtained by definitive methods developed in the Laboratory and used to support the certification process. Analytical uncertainties and stability uncertainties were quantified to arrive at combined uncertainties of the certified

  15. Analysis of current research addressing complementary use of life-cycle assessment and risk assessment for engineered nanomaterials: have lessons been learned from previous experience with chemicals?

    DEFF Research Database (Denmark)

    Grieger, Khara Deanne; Laurent, Alexis; Miseljic, Mirko

    2012-01-01

    While it is generally agreed that successful strategies to address the health and environmental impacts of engineered nanomaterials (NM) should consider the well-established frameworks for conducting life-cycle assessment (LCA) and risk assessment (RA), scientific research, and specific guidance...... on how to practically apply these methods are still very much under development. This paper evaluates how research efforts have applied LCA and RA together for NM, particularly reflecting on previous experiences with applying these methods to chemicals. Through a literature review and a separate analysis...... of research focused on applying LCA and RA together for NM, it appears that current research efforts have taken into account some key ‘‘lessons learned’’ from previous experience with chemicals while many key challenges remain for practically applying these methods to NM. We identified two main approaches...

  16. Analysis of FDA in-house food reference materials with anticoincidence INAA

    International Nuclear Information System (INIS)

    Anderson, D.L.; Cunningham, W.C.

    2013-01-01

    In-house reference material (IRM) cocoa powder (CCP) has been in use at US Food and Drug Administration laboratories for about 15 years. A single lot of commercial material was originally characterized for 32 elements by several laboratories and five techniques. A unique approach for basis weight determination based upon ambient relative humidity was developed for CCP, eliminating the need for dry weight determinations. The CCP Reference Sheet is updated by incorporating new results approximately every 5 years. The last update occurred in 2006. As part of an effort to revalidate and update values for CCP, anticoincidence instrumental neutron activation analysis (INAA) was used to determine mass fractions for 16 of the originally characterized elements, as well as to provide information on 16 other elements. Results were in very good agreement with 2006 Reference Sheet values. A new candidate IRM, fresh-frozen swordfish (FFSF) powder, was produced by adding inorganic As, Cd, Cr, Hg, Pb, Sb, and Se to liquid nitrogen-frozen commercial swordfish filets which were then homogenized. Portions of FFSF were analyzed by INAA to provide mass fraction and homogeneity information for As, Cd, Cr, Hg, Sb, and Se as well as for eight other elements occurring naturally in the material. Non-homogeneities were ≤2.5 % for As, Br, Cd, and Cs, and ≤1.8 % for Cr, Hg, Rb, Sb, and Se. Certified reference materials DORM-3 Fish Protein powder and fresh-frozen SRM 1947 Lake Michigan Fish Tissue were analyzed as controls. (author)

  17. Quantitative analysis of [18F]FDDNP PET using subcortical white matter as reference region

    International Nuclear Information System (INIS)

    Wong, Koon-Pong; Shao, Weber; Dahlbom, Magnus; Kepe, Vladimir; Liu, Jie; Satyamurthy, Nagichettiar; Barrio, Jorge R.; Wardak, Mirwais; Huang, Sung-Cheng; Small, Gary W.

    2010-01-01

    Subcortical white matter is known to be relatively unaffected by amyloid deposition in Alzheimer's disease (AD). We investigated the use of subcortical white matter as a reference region to quantify [ 18 F]FDDNP binding in the human brain. Dynamic [ 18 F]FDDNP PET studies were performed on 7 control subjects and 12 AD patients. Population efflux rate constants (k ' 2 ) from subcortical white matter (centrum semiovale) and cerebellar cortex were derived by a simplified reference tissue modeling approach incorporating physiological constraints. Regional distribution volume ratio (DVR) estimates were derived using Logan and simplified reference tissue approaches, with either subcortical white matter or cerebellum as reference input. Discriminant analysis with cross-validation was performed to classify control subjects and AD patients. The population estimates of k ' 2 in subcortical white matter did not differ significantly between control subjects and AD patients but the variability of individual estimates of k ' 2 determined in white matter was lower than that in cerebellum. Logan DVR showed dependence on the efflux rate constant in white matter. The DVR estimates in the frontal, parietal, posterior cingulate, and temporal cortices were significantly higher in the AD group (p 18 F]FDDNP with the Logan method which allows more accurate and less biased binding estimates, but a population efflux rate constant has to be determined a priori. (orig.)

  18. Software reference for SaTool - a Tool for Structural Analysis of Automated Systems

    DEFF Research Database (Denmark)

    Lorentzen, Torsten; Blanke, Mogens

    2004-01-01

    . The list of such variables and functional relations constitute the system’s structure graph. Normal operation means all functional relations are intact. Should faults occur, one or more functional relations cease to be valid. In a structure graph, this is seen as the disappearance of one or more nodes...... of the graph. SaTool makes analysis of the structure graph to provide knowledge about fundamental properties of the system in normal and faulty conditions. Salient features of SaTool include rapid analysis of possibility to diagnose faults and ability to make autonomous recovery should faults occur.......This software reference details the functions of SaTool – a tool for structural analysis of technical systems. SaTool is intended used as part of an industrial systems design cycle. Structural analysis is a graph-based technique where principal relations between variables express the system’s...

  19. GMOMETHODS: the European Union database of reference methods for GMO analysis.

    Science.gov (United States)

    Bonfini, Laura; Van den Bulcke, Marc H; Mazzara, Marco; Ben, Enrico; Patak, Alexandre

    2012-01-01

    In order to provide reliable and harmonized information on methods for GMO (genetically modified organism) analysis we have published a database called "GMOMETHODS" that supplies information on PCR assays validated according to the principles and requirements of ISO 5725 and/or the International Union of Pure and Applied Chemistry protocol. In addition, the database contains methods that have been verified by the European Union Reference Laboratory for Genetically Modified Food and Feed in the context of compliance with an European Union legislative act. The web application provides search capabilities to retrieve primers and probes sequence information on the available methods. It further supplies core data required by analytical labs to carry out GM tests and comprises information on the applied reference material and plasmid standards. The GMOMETHODS database currently contains 118 different PCR methods allowing identification of 51 single GM events and 18 taxon-specific genes in a sample. It also provides screening assays for detection of eight different genetic elements commonly used for the development of GMOs. The application is referred to by the Biosafety Clearing House, a global mechanism set up by the Cartagena Protocol on Biosafety to facilitate the exchange of information on Living Modified Organisms. The publication of the GMOMETHODS database can be considered an important step toward worldwide standardization and harmonization in GMO analysis.

  20. Apixaban compared with warfarin in patients with atrial fibrillation and previous stroke or transient ischaemic attack: a subgroup analysis of the ARISTOTLE trial.

    Science.gov (United States)

    Easton, J Donald; Lopes, Renato D; Bahit, M Cecilia; Wojdyla, Daniel M; Granger, Christopher B; Wallentin, Lars; Alings, Marco; Goto, Shinya; Lewis, Basil S; Rosenqvist, Mårten; Hanna, Michael; Mohan, Puneet; Alexander, John H; Diener, Hans-Christoph

    2012-06-01

    In the ARISTOTLE trial, the rate of stroke or systemic embolism was reduced by apixaban compared with warfarin in patients with atrial fibrillation (AF). Patients with AF and previous stroke or transient ischaemic attack (TIA) have a high risk of stroke. We therefore aimed to assess the efficacy and safety of apixaban compared with warfarin in prespecified subgroups of patients with and without previous stroke or TIA. Between Dec 19, 2006, and April 2, 2010, patients were enrolled in the ARISTOTLE trial at 1034 clinical sites in 39 countries. 18,201 patients with AF or atrial flutter were randomly assigned to receive apixaban 5 mg twice daily or warfarin (target international normalised ratio 2·0-3·0). The median duration of follow-up was 1·8 years (IQR 1·4-2·3). The primary efficacy outcome was stroke or systemic embolism, analysed by intention to treat. The primary safety outcome was major bleeding in the on-treatment population. All participants, investigators, and sponsors were masked to treatment assignments. In this subgroup analysis, we estimated event rates and used Cox models to compare outcomes in patients with and without previous stroke or TIA. The ARISTOTLE trial is registered with ClinicalTrials.gov, number NTC00412984. Of the trial population, 3436 (19%) had a previous stroke or TIA. In the subgroup of patients with previous stroke or TIA, the rate of stroke or systemic embolism was 2·46 per 100 patient-years of follow-up in the apixaban group and 3·24 in the warfarin group (hazard ratio [HR] 0·76, 95% CI 0·56 to 1·03); in the subgroup of patients without previous stroke or TIA, the rate of stroke or systemic embolism was 1·01 per 100 patient-years of follow-up with apixaban and 1·23 with warfarin (HR 0·82, 95% CI 0·65 to 1·03; p for interaction=0·71). The absolute reduction in the rate of stroke and systemic embolism with apixaban versus warfarin was 0·77 per 100 patient-years of follow-up (95% CI -0·08 to 1·63) in patients with

  1. Prepare of microanalysis reference material for nuclear analysis of Chinese ancient ceramic

    International Nuclear Information System (INIS)

    Feng Songlin; Xu Qing; Feng Xiangqian; Fan Dongyu; Lei Yong; Cheng Lin

    2005-01-01

    Some analytic technique can play important role for identifying the provenance and age of ceramic ware. However, it is usually not allowed to destructive analyze for a valuable intact porcelain ware. These analysis methods such as X-ray Fluorescence (XRF), Proton Induced X-ray Emission (PIXE), and Synchrotron Radiation X-ray Fluorescence (SRXRF) are suitable for nondestructive analysis of ancient ceramic wares. In order to compare the analytic data obtained by different measuring method and identify the provenance and age accurately, the effective way is to calibrate elemental concentration in body and glaze of ceramic ware. Microanalysis reference material (MRM) of ancient ceramic has to be prepared for achieving quantitative analysis. A solid powder 99% in size of 500 mesh for microanalysis reference material (MRM) has being prepared in institute of high energy physics. The minimum analytic masses of 1 mg were determined by Neutron Activation Analysis (NAA) for these elements (Sc, Cr, Co, Rb: Cs, La, Ce, Nd, Sm, Tb, Yb, Lu; Hf, Ta, Th, U), and by SRXRF for elements (K, Ca, Ti, Mn, Fe, Zn; Rb, Sr).

  2. Analysis of Static and Dynamic E-Reference Content at a Multi-Campus University Shows, that Updated Content is Associated with Greater Annual Usage

    Directory of Open Access Journals (Sweden)

    Laura Costello

    2016-03-01

    Full Text Available Objective – To discover whether there is a difference in use over time between dynamically updated and changing subscription e-reference titles and collections, and static purchased e-reference titles and collections. Design – Case study. Setting – A multi-campus Canadian university with 9,200 students enrolled in both graduate and undergraduate programs. Subjects – E-reference book packages and individual e-reference titles. Methods – The author compared data from individual e-reference books and packages. First, individual subscription e-reference books that periodically added updated content were compared to individually purchased e-reference books that remained static after purchase. The author then compared two e-reference book packages that provided new and updated content to two static e-reference book packages. The author compared data from patron usage to new content added over time using regression analysis. Main Results – As the library acquired e-reference titles, dynamic title subscriptions added to the collection were associated with 2,246 to 4,635 views per subscription while static title additions were associated with 8 to 123 views per purchase. The author also found that there was a strong linear relationship between views and dynamic titles added to the collection (R2=0.79 and a very weak linear relationship (R2=0.18 with views when static titles are added to the collection. Regression analysis of dynamic e-reference collections revealed that the number of titles added to each collection was strongly associated with views of the material (R2=0.99, while static e-reference collections were less strongly linked (R2=0.43. Conclusion – Dynamic e-reference titles and collections experienced increases in usage each year while static titles and collections experienced decreases in usage. This indicates that collections and titles that offer new content to users each year will continue to see growth in usage while static

  3. Preparation and evaluation of reference materials for accountancy analysis. (1) Preparation and evaluation method

    International Nuclear Information System (INIS)

    Takamatsu, Mai; Kacchi, Tomokazu; Murakami, Toshiki; Ai, Hironobu; Sumi, Mika; Abe, Katsuo; Kageyama, Tomio; Nakazawa, Hiroaki

    2009-01-01

    Isotope dilution mass spectrometry method used for the accountancy analysis at nuclear fuel facilities requires the standard materials called LSD (Large Size Dried) spike. Generally, LSD spikes are prepared from certified reference materials (CRMs) which supplied from foreign laboratories. However, the difficulty of Pu CRM importation is increasing. It is important for safeguards to attain and continue high reliable accountancy analysis and stable securing of LSD spike is essential. Therefore, in order to conserve CRMs, several types of LSD spike were prepared under collaboration work between JAEA and JNFL, such as the amount of nuclear material in one LSD spike is decreased and others. Practical test with actual samples were performed at JNFL Rokkasho reprocessing plant, and those results were compared with the results obtained by using LSD spike which supplied from foreign laboratory. Preparation and verification analysis of LSD spikes and evaluation of uncertainty based on ISO-GUM will be presented. (author)

  4. Molecular analysis of clinical isolates previously diagnosed as Mycobacterium intracellulare reveals incidental findings of "Mycobacterium indicus pranii" genotypes in human lung infection.

    Science.gov (United States)

    Kim, Su-Young; Park, Hye Yun; Jeong, Byeong-Ho; Jeon, Kyeongman; Huh, Hee Jae; Ki, Chang-Seok; Lee, Nam Yong; Han, Seung-Jung; Shin, Sung Jae; Koh, Won-Jung

    2015-09-30

    Mycobacterium intracellulare is a major cause of Mycobacterium avium complex lung disease in many countries. Molecular studies have revealed several new Mycobacteria species that are closely related to M. intracellulare. The aim of this study was to re-identify and characterize clinical isolates from patients previously diagnosed with M. intracellulare lung disease at the molecular level. Mycobacterial isolates from 77 patients, initially diagnosed with M. intracellulare lung disease were re-analyzed by multi-locus sequencing and pattern of insertion sequences. Among the 77 isolates, 74 (96 %) isolates were designated as M. intracellulare based on multigene sequence-based analysis. Interestingly, the three remaining strains (4 %) were re-identified as "Mycobacterium indicus pranii" according to distinct molecular phylogenetic positions in rpoB and hsp65 sequence-based typing. In hsp65 sequevar analysis, code 13 was found in the majority of cases and three unreported codes were identified. In 16S-23S rRNA internal transcribed spacer (ITS) sequevar analysis, all isolates of both species were classified within the Min-A ITS sequevar. Interestingly, four of the M. intracellulare isolates harbored IS1311, a M. avium-specific element. Two of three patients infected with "M. indicus pranii" had persistent positive sputum cultures after antibiotic therapy, indicating the clinical relevance of this study. This analysis highlights the importance of precise identification of clinical isolates genetically close to Mycobacterium species, and suggests that greater attention should be paid to nontuberculous mycobacteria lung disease caused by "M. indicus pranii".

  5. Reference-free total reflection X-ray fluorescence analysis of semiconductor surfaces with synchrotron radiation.

    Science.gov (United States)

    Beckhoff, Burkhard; Fliegauf, Rolf; Kolbe, Michael; Müller, Matthias; Weser, Jan; Ulm, Gerhard

    2007-10-15

    Total reflection X-ray fluorescence (TXRF) analysis is a well-established method to monitor lowest level contamination on semiconductor surfaces. Even light elements on a wafer surface can be excited effectively when using high-flux synchrotron radiation in the soft X-ray range. To meet current industrial requirements in nondestructive semiconductor analysis, the Physikalisch-Technische Bundesanstalt (PTB) operates dedicated instrumentation for analyzing light element contamination on wafer pieces as well as on 200- and 300-mm silicon wafer surfaces. This instrumentation is also suited for grazing incidence X-ray fluorescence analysis and conventional energy-dispersive X-ray fluorescence analysis of buried and surface nanolayered structures, respectively. The most prominent features are a high-vacuum load-lock combined with an equipment front end module and a UHV irradiation chamber with an electrostatic chuck mounted on an eight-axis manipulator. Here, the entire surface of a 200- or a 300-mm wafer can be scanned by monochromatized radiation provided by the plane grating monochromator beamline for undulator radiation in the PTB laboratory at the electron storage ring BESSY II. This beamline provides high spectral purity and high photon flux in the range of 0.078-1.86 keV. In addition, absolutely calibrated photodiodes and Si(Li) detectors are used to monitor the exciting radiant power respectively the fluorescence radiation. Furthermore, the footprint of the excitation radiation at the wafer surface is well-known due to beam profile recordings by a CCD during special operation conditions at BESSY II that allow for drastically reduced electron beam currents. Thus, all the requirements of completely reference-free quantitation of TXRF analysis are fulfilled and are to be presented in the present work. The perspectives to arrange for reference-free quantitation using X-ray tube-based, table-top TXRF analysis are also addressed.

  6. Analysis of cyclic creep and rupture. Part 1: bounding theorems and cyclic reference stresses

    Energy Technology Data Exchange (ETDEWEB)

    Carter, P. [Stress Engineering Services, Inc., Mason, OH 45040 (United States)]. E-mail: peter.carter@ses-oh.com

    2005-01-01

    Cyclic loading on structures can produce failures not readily predicted by conventional static analysis. Ratcheting or incremental distortion leads to structural failure, and complicates the problems of creep and fatigue prediction. Predicting shakedown, ratcheting, accelerated creep and rupture, for cyclic loading, are the objectives of cyclic stress analysis.Limit load, shakedown and ratcheting analyses provide a comprehensive basis to understand static structural behaviour for ductile inelastic materials, subject to variable loading but excluding inertial dynamic effects. From them we can predict the following failure modes:-Plastic collapse.-Failure to shakedown.-Ratcheting.-Accelerated creep and rupture.This is achieved with a generalisation of the reference stress concept. Conventionally, and for steady loading, the limit load reference stress is the lowest yield stress for which the structure does not collapse. For cyclic loading two definitions are available. The more conservative is the lowest yield stress for which the structure shakes down (behaves elastically). The less conservative is the lowest yield stress for which the structure does not ratchet. They have different meanings and uses.Explaining and justifying the use of cyclic reference stresses to bound creep and rupture is the objective of Part 1. Part 2 gives examples illustrating a range of structural behaviours. The methodology of these papers involves so-called approximate methods at one level, that of inferring limiting or conservative time-dependent behaviour from time-independent elastic-plastic cyclic analyses. The elastic-plastic cyclic analyses themselves are straightforward if tedious. Some ideas and a new analysis technique are available to reduce the trial-and-error.

  7. An update to the analysis of the Canadian Spatial Reference System

    Science.gov (United States)

    Ferland, R.; Piraszewski, M.; Craymer, M.

    2015-12-01

    The primary objective of the Canadian Spatial Reference System (CSRS) is to provide users access to a consistent geo-referencing infrastructure over the Canadian landmass. Global Navigation Satellite System (GNSS) positioning accuracy requirements ranges from meter level to mm level (e.g.: crustal deformation). The highest level of the Canadian infrastructure consist of a network of continually operating GPS and GNSS receivers, referred to as active control stations. The network includes all Canadian public active control stations, some bordering US CORS and Alaska stations, Greenland active control stations, as well as a selection of IGS reference frame stations. The Bernese analysis software is used for the daily processing and the combination into weekly solutions which form the basis for this analysis. IGS weekly final orbit, Earth Rotation parameters (ERP's) and coordinates products are used in the processing. For the more demanding users, the time dependant changes of station coordinates is often more important.All station coordinate estimates and related covariance information is used in this analysis. For each input solution, variance factor, translation, rotation and scale (and if needed their rates) or subsets of these are estimated. In the combination of these weekly solutions, station positions and velocities are estimated. Since the time series from the stations in these networks often experience changes in behavior, new (or reuse of) parameters are generally used in these situations. As is often the case with real data, unrealistic coordinates may occur. Automatic detection and removal of outliers is used in these cases. For the transformation, position and velocity parameters loose apriori estimates and uncertainties are provided. Alignment using the usual Helmert transformation to the latest IGb08 realization of ITRF is also performed during the adjustment.

  8. Washout allometric reference method (WARM) for parametric analysis of [11C]PIB in human brains

    Science.gov (United States)

    Rodell, Anders; Aanerud, Joel; Braendgaard, Hans; Gjedde, Albert

    2013-01-01

    Rapid clearance and disappearance of a tracer from the circulation challenges the determination of the tracer's binding potentials in brain (BPND) by positron emission tomography (PET). This is the case for the analysis of the binding of radiolabeled [11C]Pittsburgh Compound B ([11C]PIB) to amyloid-β (Aβ) plaques in brain of patients with Alzheimer's disease (AD). To resolve the issue of rapid clearance from the circulation, we here introduce the flow-independent Washout Allometric Reference Method (WARM) for the analysis of washout and binding of [11C]PIB in two groups of human subjects, healthy aged control subjects (HC), and patients suffering from AD, and we compare the results to the outcome of two conventional analysis methods. We also use the rapid initial clearance to obtain a surrogate measure of the rate of cerebral blood flow (CBF), as well as a method of identifying a suitable reference region directly from the [11C]PIB signal. The difference of average absolute CBF values between the AD and HC groups was highly significant (P PIB binding, the separate estimates of CBF and BPND provide additional information about possible AD. The results demonstrate the importance of data-driven estimation of CBF and BPND, as well as reference region detection from the [11C]PIB signal. We conclude that the WARM method yields stable measures of BPND with relative ease, using only integration for noise reduction and no model regression. The method accounts for relative flow differences in the brain tissue and yields a calibrated measure of absolute CBF directly from the [11C]PIB signal. Compared to conventional methods, WARM optimizes the Aβ plaque load discrimination between patients with AD and healthy controls (P = 0.009). PMID:24348416

  9. Washout allometric reference method (WARM) for parametric analysis of [(11)C]PIB in human brains.

    Science.gov (United States)

    Rodell, Anders; Aanerud, Joel; Braendgaard, Hans; Gjedde, Albert

    2013-01-01

    Rapid clearance and disappearance of a tracer from the circulation challenges the determination of the tracer's binding potentials in brain (BP ND) by positron emission tomography (PET). This is the case for the analysis of the binding of radiolabeled [(11)C]Pittsburgh Compound B ([(11)C]PIB) to amyloid-β (Aβ) plaques in brain of patients with Alzheimer's disease (AD). To resolve the issue of rapid clearance from the circulation, we here introduce the flow-independent Washout Allometric Reference Method (WARM) for the analysis of washout and binding of [(11)C]PIB in two groups of human subjects, healthy aged control subjects (HC), and patients suffering from AD, and we compare the results to the outcome of two conventional analysis methods. We also use the rapid initial clearance to obtain a surrogate measure of the rate of cerebral blood flow (CBF), as well as a method of identifying a suitable reference region directly from the [(11)C]PIB signal. The difference of average absolute CBF values between the AD and HC groups was highly significant (P PIB binding, the separate estimates of CBF and BP ND provide additional information about possible AD. The results demonstrate the importance of data-driven estimation of CBF and BP ND, as well as reference region detection from the [(11)C]PIB signal. We conclude that the WARM method yields stable measures of BP ND with relative ease, using only integration for noise reduction and no model regression. The method accounts for relative flow differences in the brain tissue and yields a calibrated measure of absolute CBF directly from the [(11)C]PIB signal. Compared to conventional methods, WARM optimizes the Aβ plaque load discrimination between patients with AD and healthy controls (P = 0.009).

  10. Usability Testing Analysis on The Bana Game as Education Game Design References on Junior High School

    Directory of Open Access Journals (Sweden)

    F. Adnan

    2017-04-01

    Full Text Available Learning media is one of the important elements in the learning process. Technological development support makes learning media more varied. The approach of using digital technology as a learning media has a better and more effective impact than other approaches. In order to increase the students’ learning interest, it requires the support of an interesting learning media. The use of gaming applications as learning media can improve learning outcomes. The benefits of using the maximum application cannot be separated from the determination of application design. The Bana game aims to increase the ability of critical thinking of the junior high school students. The usability-testing analysis on the Bana game application is used in order to get the design reference as an educational game development. The game is used as an object of the analysis because it has the same characteristics and goals with the game application to be developed. Usability Testing is a method used to measure the ease of use of an application by users. The Usability Testing consists of learnability, efficiency, memorability, errors, and satisfaction. The results of the analysis obtained will be used as a reference for educational game applications that will be developed.

  11. Challenges in the size analysis of a silica nanoparticle mixture as candidate certified reference material

    International Nuclear Information System (INIS)

    Kestens, Vikram; Roebben, Gert; Herrmann, Jan; Jämting, Åsa; Coleman, Victoria; Minelli, Caterina; Clifford, Charles; Temmerman, Pieter-Jan De; Mast, Jan; Junjie, Liu; Babick, Frank; Cölfen, Helmut; Emons, Hendrik

    2016-01-01

    A new certified reference material for quality control of nanoparticle size analysis methods has been developed and produced by the Institute for Reference Materials and Measurements of the European Commission’s Joint Research Centre. The material, ERM-FD102, consists of an aqueous suspension of a mixture of silica nanoparticle populations of distinct particle size and origin. The characterisation relied on an interlaboratory comparison study in which 30 laboratories of demonstrated competence participated with a variety of techniques for particle size analysis. After scrutinising the received datasets, certified and indicative values for different method-defined equivalent diameters that are specific for dynamic light scattering (DLS), centrifugal liquid sedimentation (CLS), scanning and transmission electron microscopy (SEM and TEM), atomic force microscopy (AFM), particle tracking analysis (PTA) and asymmetrical-flow field-flow fractionation (AF4) were assigned. The value assignment was a particular challenge because metrological concepts were not always interpreted uniformly across all participating laboratories. This paper presents the main elements and results of the ERM-FD102 characterisation study and discusses in particular the key issues of measurand definition and the estimation of measurement uncertainty.

  12. Determination of arsenic in food and dietary supplement standard reference materials by neutron activation analysis

    International Nuclear Information System (INIS)

    Paul, R.L.

    2013-01-01

    Arsenic was measured in food and dietary supplement standard reference materials by neutron activation analysis for the purpose of assigning certified or reference As mass fractions and to assess material homogeneity. Instrumental neutron activation analysis was used to value assign As in candidate SRM 3532 Calcium Dietary Supplement and candidate SRM 3262 Hypericum perforatum (St. John's Wort) Aerial Parts down to about 100 μg/kg. Values were also determined for two additional candidate St. John's Wort SRMs with As mass fractions 24 Na and 82 Br limited the reproducibility of the method below 100 μg/kg. For measurement of lower As mass fractions, a radiochemical neutron activation analysis method with extraction of As 3+ into diethyl-dithiocarbamate in chloroform and detection limits down to 0.1 μg/kg. As was used to value-assign As mass fractions for SRM 3280 Multivitamin/Multielement Tablets and for candidate SRM 3233 Fortified Breakfast Cereal, and at <10 μg/kg in candidate SRM 1845a Whole Egg Powder. (author)

  13. Challenges in the size analysis of a silica nanoparticle mixture as candidate certified reference material

    Energy Technology Data Exchange (ETDEWEB)

    Kestens, Vikram, E-mail: vikram.kestens@ec.europa.eu; Roebben, Gert [Joint Research Centre (JRC), European Commission, Institute for Reference Materials and Measurements (IRMM) (Belgium); Herrmann, Jan; Jämting, Åsa; Coleman, Victoria [National Measurement Institute Australia, Nanometrology Section (Australia); Minelli, Caterina; Clifford, Charles [National Physical Laboratory, Analytical Science Division (United Kingdom); Temmerman, Pieter-Jan De; Mast, Jan [Service Electron Microscopy, Veterinary and Agrochemical Research Centre (CODA-CERVA) (Belgium); Junjie, Liu [National Institute of Metrology, Division of Nanoscale Measurement and Advanced Materials (China); Babick, Frank [Technische Universität Dresden, Institut für Verfahrens- und Umwelttechnik (Germany); Cölfen, Helmut [University of Konstanz, Physical Chemistry, Department of Chemistry (Germany); Emons, Hendrik [Joint Research Centre (JRC), European Commission, Institute for Reference Materials and Measurements (IRMM) (Belgium)

    2016-06-15

    A new certified reference material for quality control of nanoparticle size analysis methods has been developed and produced by the Institute for Reference Materials and Measurements of the European Commission’s Joint Research Centre. The material, ERM-FD102, consists of an aqueous suspension of a mixture of silica nanoparticle populations of distinct particle size and origin. The characterisation relied on an interlaboratory comparison study in which 30 laboratories of demonstrated competence participated with a variety of techniques for particle size analysis. After scrutinising the received datasets, certified and indicative values for different method-defined equivalent diameters that are specific for dynamic light scattering (DLS), centrifugal liquid sedimentation (CLS), scanning and transmission electron microscopy (SEM and TEM), atomic force microscopy (AFM), particle tracking analysis (PTA) and asymmetrical-flow field-flow fractionation (AF4) were assigned. The value assignment was a particular challenge because metrological concepts were not always interpreted uniformly across all participating laboratories. This paper presents the main elements and results of the ERM-FD102 characterisation study and discusses in particular the key issues of measurand definition and the estimation of measurement uncertainty.

  14. Risk of stillbirth, preterm delivery, and fetal growth restriction following exposure in a previous birth: systematic review and meta-analysis.

    Science.gov (United States)

    Malacova, E; Regan, A; Nassar, N; Raynes-Greenow, C; Leonard, H; Srinivasjois, R; W Shand, A; Lavin, T; Pereira, G

    2018-01-01

    Little is known about the risk of non-recurrent adverse birth outcomes. To evaluate the risk of stillbirth, preterm birth (PTB), and small for gestational age (SGA) as a proxy for fetal growth restriction (FGR) following exposure to one or more of these factors in a previous birth. We searched MEDLINE, EMBASE, Maternity and Infant Care, and Global Health from inception to 30 November 2016. Studies were included if they investigated the association between stillbirth, PTB, or SGA (as a proxy for FGR) in two subsequent births. Meta-analysis and pooled association presented as odds ratios (ORs) and adjusted odds ratios (aORs). Of the 3399 studies identified, 17 met the inclusion criteria. A PTB or SGA (as a proxy for FGR) infant increased the risk of subsequent stillbirth ((pooled OR 1.70; 95% confidence interval, 95% CI, 1.34-2.16) and (pooled OR 1.98; 95% CI 1.70-2.31), respectively). A combination of exposures, such as a preterm SGA (as a proxy for FGR) birth, doubled the risk of subsequent stillbirth (pooled OR 4.47; 95% CI 2.58-7.76). The risk of stillbirth also varied with prematurity, increasing three-fold following PTB preterm SGA (as a proxy for FGR) preterm (preterm or small for gestational age can increase the chance of long-term health problems. The effect of having a stillbirth, preterm birth, or small-for-gestational-age infant in a previous pregnancy on future pregnancy health has not been summarised. We identified 3399 studies of outcomes of previous pregnancies, and 17 were summarised by our study. What were the main findings? The outcome of the previous pregnancy influenced the risk of poor outcomes in the next pregnancy. Babies born to mothers who had a previous preterm birth or small-for-gestational-age birth were more likely to be stillborn. The smaller and the more preterm the previous baby, the higher the risk of stillbirth in the following pregnancy. The risk of stillbirth in the following pregnancy was doubled if the previous baby was born

  15. Collaborative ring trial of the papaya endogenous reference gene and its polymerase chain reaction assays for genetically modified organism analysis.

    Science.gov (United States)

    Wei, Jiaojun; Li, Feiwu; Guo, Jinchao; Li, Xiang; Xu, Junfeng; Wu, Gang; Zhang, Dabing; Yang, Litao

    2013-11-27

    The papaya (Carica papaya L.) Chymopapain (CHY) gene has been reported as a suitable endogenous reference gene for genetically modified (GM) papaya detection in previous studies. Herein, we further validated the use of the CHY gene and its qualitative and quantitative polymerase chain reaction (PCR) assays through an interlaboratory collaborative ring trial. A total of 12 laboratories working on detection of genetically modified organisms participated in the ring trial and returned test results. Statistical analysis of the returned results confirmed the species specificity, low heterogeneity, and single-copy number of the CHY gene among different papaya varieties. The limit of detection of the CHY qualitative PCR assay was 0.1%, while the limit of quantification of the quantitative PCR assay was ∼25 copies of haploid papaya genome with acceptable PCR efficiency and linearity. The differences between the tested and true values of papaya content in 10 blind samples ranged from 0.84 to 6.58%. These results indicated that the CHY gene was suitable as an endogenous reference gene for the identification and quantification of GM papaya.

  16. Characterisation of candidate reference materials by PIXE analysis and nuclear microprobe PIXE imaging

    International Nuclear Information System (INIS)

    Jaksic, M.; Pastuovic, Z.; Bogdanovic, I.; Tadic, T.

    2002-01-01

    In order to test whether some candidate reference materials show homogeneity that can satisfy quality control of the PIXE technique, six bottles of each of the two Candidate RM's - Lichen (IAEA 338) and Algae (IAEA 413) were tested. Four different tests were performed. First, two pellets from each bottle were prepared and analysed using broad beam (φ = 5 mm) PIXE. Second and third was analysis of homogeneity using scanning focussed beam at the nuclear microprobe. Scans of 50x50 μm 2 and 240x260 μm 2 were performed. Finally, individual grains with composition differing from the rest of the sample, were analysed using PIXE and RBS. (author)

  17. Laparoscopy After Previous Laparotomy

    Directory of Open Access Journals (Sweden)

    Zulfo Godinjak

    2006-11-01

    Full Text Available Following the abdominal surgery, extensive adhesions often occur and they can cause difficulties during laparoscopic operations. However, previous laparotomy is not considered to be a contraindication for laparoscopy. The aim of this study is to present that an insertion of Veres needle in the region of umbilicus is a safe method for creating a pneumoperitoneum for laparoscopic operations after previous laparotomy. In the last three years, we have performed 144 laparoscopic operations in patients that previously underwent one or two laparotomies. Pathology of digestive system, genital organs, Cesarean Section or abdominal war injuries were the most common causes of previouslaparotomy. During those operations or during entering into abdominal cavity we have not experienced any complications, while in 7 patients we performed conversion to laparotomy following the diagnostic laparoscopy. In all patients an insertion of Veres needle and trocar insertion in the umbilical region was performed, namely a technique of closed laparoscopy. Not even in one patient adhesions in the region of umbilicus were found, and no abdominal organs were injured.

  18. Evaluation of ABS resin disk certified reference materials for heavy metal analysis by x-ray fluorescence analysis

    International Nuclear Information System (INIS)

    Ohata, Masaki; Kidokoro, Toshihiro; Kurahashi, Masayasu; Hioki, Akiharu

    2010-01-01

    ABS resin disk certified reference materials (CRMs) for heavy-metal analysis (NMIJ CRM 8105-a, NMIJ CRM 8106-a, NMIJ CRM 8115-a and NMIJ CRM 8116-a) were evaluated using an energy dispersive X-ray fluorescence (ED-XRF) analysis. The homogeneities of elements for both among-disks and within-disk were evaluated by ED-XRF analysis without any sample pre-treatment, which were similar to those evaluated by ICP-MS analysis after a sample digenstion procedure. The normalized XRF sensitivities for Cd, Cr and Pb in different ABS resin disk CRMs were compared, and the differences among them for those ABS resin disks that have similar matrices were observed. Moreover, Hg in those ABS resin disk CRMs was stable for long-term X-ray irradiation during ED-XRF analysis. (author)

  19. Gene Expression Profile Analysis is Directly Affected by the Selected Reference Gene: The Case of Leaf-Cutting Atta Sexdens

    Directory of Open Access Journals (Sweden)

    Kalynka G. do Livramento

    2018-02-01

    Full Text Available Although several ant species are important targets for the development of molecular control strategies, only a few studies focus on identifying and validating reference genes for quantitative reverse transcription polymerase chain reaction (RT-qPCR data normalization. We provide here an extensive study to identify and validate suitable reference genes for gene expression analysis in the ant Atta sexdens, a threatening agricultural pest in South America. The optimal number of reference genes varies according to each sample and the result generated by RefFinder differed about which is the most suitable reference gene. Results suggest that the RPS16, NADH and SDHB genes were the best reference genes in the sample pool according to stability values. The SNF7 gene expression pattern was stable in all evaluated sample set. In contrast, when using less stable reference genes for normalization a large variability in SNF7 gene expression was recorded. There is no universal reference gene suitable for all conditions under analysis, since these genes can also participate in different cellular functions, thus requiring a systematic validation of possible reference genes for each specific condition. The choice of reference genes on SNF7 gene normalization confirmed that unstable reference genes might drastically change the expression profile analysis of target candidate genes.

  20. Analysis of current research addressing complementary use of life-cycle assessment and risk assessment for engineered nanomaterials: have lessons been learned from previous experience with chemicals?

    International Nuclear Information System (INIS)

    Grieger, Khara D.; Laurent, Alexis; Miseljic, Mirko; Christensen, Frans; Baun, Anders; Olsen, Stig I.

    2012-01-01

    While it is generally agreed that successful strategies to address the health and environmental impacts of engineered nanomaterials (NM) should consider the well-established frameworks for conducting life-cycle assessment (LCA) and risk assessment (RA), scientific research, and specific guidance on how to practically apply these methods are still very much under development. This paper evaluates how research efforts have applied LCA and RA together for NM, particularly reflecting on previous experiences with applying these methods to chemicals. Through a literature review and a separate analysis of research focused on applying LCA and RA together for NM, it appears that current research efforts have taken into account some key “lessons learned” from previous experience with chemicals while many key challenges remain for practically applying these methods to NM. We identified two main approaches for using these methods together for NM: “LC-based RA” (traditional RA applied in a life-cycle perspective) and “RA-complemented LCA” (conventional LCA supplemented by RA in specific life-cycle steps). Hence, the latter is the only identified approach which genuinely combines LC- and RA-based methods for NM-risk research efforts to date as the former is rather a continuation of normal RA according to standard assessment procedures (e.g., REACH). Both these approaches along with recommendations for using LCA and RA together for NM are similar to those made previously for chemicals, and thus, there does not appear to be much progress made specific for NM. We have identified one issue in particular that may be specific for NM when applying LCA and RA at this time: the need to establish proper dose metrics within both methods.

  1. Potential protective effect of lactation against incidence of type 2 diabetes mellitus in women with previous gestational diabetes mellitus: A systematic review and meta-analysis.

    Science.gov (United States)

    Tanase-Nakao, Kanako; Arata, Naoko; Kawasaki, Maki; Yasuhi, Ichiro; Sone, Hirohito; Mori, Rintaro; Ota, Erika

    2017-05-01

    Lactation may protect women with previous gestational diabetes mellitus (GDM) from developing type 2 diabetes mellitus, but the results of existing studies are inconsistent, ranging from null to beneficial. We aimed to conduct a systematic review to gather available evidence. Databases MEDLINE, CINAHL, PubMed, and EMBASE were searched on December 15, 2015, without restriction of language or publication year. A manual search was also conducted. We included observational studies (cross-sectional, case-control, and cohort study) with information on lactation and type 2 diabetes mellitus incidence among women with previous GDM. We excluded case studies without control data. Data synthesis was conducted by random-effect meta-analysis. Fourteen reports of 9 studies were included. Overall risk of bias using RoBANS ranged from low to unclear. Longer lactation for more than 4 to 12 weeks postpartum had risk reduction of type 2 diabetes mellitus compared with shorter lactation (OR 0.77, 95% CI 0.01-55.86; OR 0.56, 95% CI 0.35-0.89; OR 0.22, 95% CI 0.13-0.36; type 2 diabetes mellitus evaluation time 2 y, 2-5 y, and >5 y, respectively). Exclusive lactation for more than 6 to 9 weeks postpartum also had lower risk of type 2 diabetes mellitus compared with exclusive formula (OR 0.42, 95% CI 0.22-0.81). The findings support the evidence that longer and exclusive lactation may be beneficial for type 2 diabetes mellitus prevention in women with previous GDM. However, the evidence relies only on observational studies. Therefore, further studies are required to address the true causal effect. © 2017 The Authors. Diabetes/Metabolism Research and Reviews Published by John Wiley & Sons Ltd.

  2. Reference manual for generation and analysis of Habitat Time Series: version II

    Science.gov (United States)

    Milhous, Robert T.; Bartholow, John M.; Updike, Marlys A.; Moos, Alan R.

    1990-01-01

    The selection of an instream flow requirement for water resource management often requires the review of how the physical habitat changes through time. This review is referred to as 'Time Series Analysis." The Tune Series Library (fSLIB) is a group of programs to enter, transform, analyze, and display time series data for use in stream habitat assessment. A time series may be defined as a sequence of data recorded or calculated over time. Examples might be historical monthly flow, predicted monthly weighted usable area, daily electrical power generation, annual irrigation diversion, and so forth. The time series can be analyzed, both descriptively and analytically, to understand the importance of the variation in the events over time. This is especially useful in the development of instream flow needs based on habitat availability. The TSLIB group of programs assumes that you have an adequate study plan to guide you in your analysis. You need to already have knowledge about such things as time period and time step, species and life stages to consider, and appropriate comparisons or statistics to be produced and displayed or tabulated. Knowing your destination, you must first evaluate whether TSLIB can get you there. Remember, data are not answers. This publication is a reference manual to TSLIB and is intended to be a guide to the process of using the various programs in TSLIB. This manual is essentially limited to the hands-on use of the various programs. a TSLIB use interface program (called RTSM) has been developed to provide an integrated working environment where the use has a brief on-line description of each TSLIB program with the capability to run the TSLIB program while in the user interface. For information on the RTSM program, refer to Appendix F. Before applying the computer models described herein, it is recommended that the user enroll in the short course "Problem Solving with the Instream Flow Incremental Methodology (IFIM)." This course is offered

  3. Reference materials and globalization of measurement information. ; Certified reference materials for environmental analysis. Keisoku joho no globalizm to hyojun busshitsu. ; Kankyo keisoku to hyojun busshitsu

    Energy Technology Data Exchange (ETDEWEB)

    Okamoto, K. (Tokushima University, Tokushima (Japan). Faculty of Pharmaceutical Sciences)

    1993-08-10

    Explanation is made of certified reference materials which are necessary for monitoring the global environment. As a medium to convert the physical quantity into the chemical quantity, the reference materials are used for calibrating the measurement instruments. Examples of standard physics are quoted. For monitoring the carbon dioxide, it is necessary to measure its content near 350ppm with an accuracy of 0.1ppm by infrared spectrophotography. Not only observation use standard gas examined with the standard gas in Scripps is generally used, but also standard gas from the Chemicals Inspection and Testing Institute, Japan as well as the NIST is distributed. The mussel watch is used for investigating the oceanic pollution. There is a method in which measurement is made of heavy alloys and organic compounds contained in the mussel. Progress is being made in the preparation of standard materials which are needed also for controlling the analysis accuracy. Necessary items for measuring the acid rain are pH, electric conductivity, Cl[sup -], NO3[sup -] and SO4[sup 2-]. Needed are reference materials which can be used for simultaneously measuring different contents in the rainwater. Also in Japan, preparation is being studied of reference materials for analyzing the rainwater. 2 refs., 2 tabs.

  4. Ameloblastic fibroma: a stage in the development of a hamartomatous odontoma or a true neoplasm? Critical analysis of 162 previously reported cases plus 10 new cases.

    Science.gov (United States)

    Buchner, Amos; Vered, Marilena

    2013-11-01

    To analyze neoplastic and hamartomatous variants of ameloblastic fibromas (AFs). Analysis of 172 cases (162 previously reported, 10 new). AF emerged as a lesion primarily of children and adolescents (mean age, 14.9 years), with about 80% diagnosed when odontogenesis is completed (age, 22 years are considered true neoplasms, while those in younger patients may be either true neoplasms or odontomas in early stages of development. Although the histopathology of hamartomatous and neoplastic variants of AF are indistinguishable, clinical and radiologic features can be of some help to distinguish between them. Asymptomatic small unilocular lesions with no or minimal bone expansion in young individuals are likely to be developing odontomas, and large, expansile lesions with extensive bone destruction are neoplasms. Copyright © 2013 Elsevier Inc. All rights reserved.

  5. Application of reference materials for quality assessment in neutron activation analysis

    International Nuclear Information System (INIS)

    Obrusnik, I.; Eckschlager, K.

    1993-01-01

    It is generally accepted that an analytical procedure can be regarded as an information production system yielding information on the composition of the analyzed sample. Thus, information theory can be useful and the quantities characterizing the information properties of an analytical method may be applied not only as evaluation criteria but also as objective functions in the optimization. The usability of information theory is demonstrated on the example of neutron activation analysis. Both precision and bias of NAA results are taken into account together with the possible use of reference materials for quality assessment. The influence of the above-mentioned parameters on information properties such as information gain and profitability of NAA results is discussed in detail. It has been proved that information theory is especially useful in choosing suitable reference materials for the quality assessment of routine analytical procedures not only with respect to matrix and analyte concentration in the sample but also to concentrations and uncertainties of certified values in the CRM used. In the extreme trace analysis, CRMs with relatively large uncertainties and very low certified concentrations can still yield rather high information gain of results. (author) 14 refs.; 9 figs

  6. Artificial oocyte activation to improve reproductive outcomes in women with previous fertilization failure: a systematic review and meta-analysis of RCTs.

    Science.gov (United States)

    Sfontouris, Ioannis A; Nastri, Carolina O; Lima, Maria L S; Tahmasbpourmarzouni, Eisa; Raine-Fenning, Nick; Martins, Wellington P

    2015-08-01

    In couples with previous fertilization failure, are reproductive outcomes improved using ICSI followed by artificial oocyte activation (ICSI-AOA) compared with conventional ICSI? There is insufficient evidence available from RCTs to judge the efficacy and safety of ICSI-AOA for couples with previous fertilization failure. In cases with previous low fertilization rates or total fertilization failure using ICSI due to sperm-related, oocyte activation deficiency, several methods of AOA have been described, which employ mechanical, electrical or chemical stimuli. Reported fertilization and pregnancy rates appear to be improved after ICSI-AOA compared with conventional ICSI; however, the small studies performed to date make it difficult to assess the clinical efficacy or safety of AOA. The present systematic review and meta-analysis identified RCTs that compared ICSI-AOA and conventional ICSI. The last electronic search was conducted in August 2014 and there was no limitation regarding language, publication date, or publication status. We included studies that randomized either oocytes or women and included them in two different parts of this review: a women-based review and an oocyte-based review. For the women-based review, the primary outcome of effectiveness was live birth per randomized woman and the primary outcome for safety was congenital anomalies per clinical pregnancy. For the oocyte-based review, the primary outcome was embryo formation per oocyte randomized. Record screening and data extraction were performed independently by two authors and risk of bias was assessed by three authors. The effects of ICSI-AOA compared with conventional ICSI were summarized as risk ratio (RR) and the precision of the estimates was evaluated by the 95% confidence interval (CI). A total of 14 articles were assessed for eligibility and 9 included in the meta-analysis: 2 studies comprised the woman-based review (n = 168 women) and 7 studies the oocyte-based review (n = 4234

  7. Recent references

    International Nuclear Information System (INIS)

    Ramavataram, S.

    1991-01-01

    In support of a continuing program of systematic evaluation of nuclear structure data, the National Nuclear Data Center maintains a complete computer file of references to the nuclear physics literature. Each reference is tagged by a keyword string, which indicates the kinds of data contained in the article. This master file of Nuclear Structure References (NSR) contains complete keyword indexes to literature published since 1969, with partial indexing of older references. Any reader who finds errors in the keyword descriptions is urged to report them to the National Nuclear Data Center so that the master NSR file can be corrected. In 1966, the first collection of Recent References was published as a separate issue of Nuclear Data Sheets. Every four months since 1970, a similar indexed bibliography to new nuclear experiments has been prepared from additions to the NSR file and published. Beginning in 1978, Recent References was cumulated annually, with the third issue completely superseding the two issues previously published during a given year. Due to publication policy changes, cumulation of Recent Reference was discontinued in 1986. The volume and issue number of all the cumulative issues published to date are given. NNDC will continue to respond to individual requests for special bibliographies on nuclear physics topics, in addition to those easily obtained from Recent References. If the required information is available from the keyword string, a reference list can be prepared automatically from the computer files. This service can be provided on request, in exchange for the timely communication of new nuclear physics results (e.g., preprints). A current copy of the NSR file may also be obtained in a standard format on magnetic tape from NNDC. Requests for special searches of the NSR file may also be directed to the National Nuclear Data Center

  8. Selection of reference genes for expression analysis of Kumamoto and Portuguese oysters and their hybrid

    Science.gov (United States)

    Yan, Lulu; Su, Jiaqi; Wang, Zhaoping; Yan, Xiwu; Yu, Ruihai

    2017-12-01

    Quantitative real-time polymerase chain reaction (qRT-PCR) is a rapid and reliable technique which has been widely used to quantifying gene transcripts (expression analysis). It is also employed for studying heterosis, hybridization breeding and hybrid tolerability of oysters, an ecologically and economically important taxonomic group. For these studies, selection of a suitable set of housekeeping genes as references is crucial for correct interpretation of qRT-PCR data. To identify suitable reference genes for oysters during low temperature and low salinity stresses, we analyzed twelve genes from the gill tissue of Crassostrea sikamea (SS), Crassostrea angulata (AA) and their hybrid (SA), which included three ribosomal genes, 28S ribosomal protein S5 ( RPS5), ribosomal protein L35 ( RPL35), and 60S ribosomal protein L29 ( RPL29); three structural genes, tubulin gamma ( TUBγ), annexin A6 and A7 ( AA6 and AA7); three metabolic pathway genes, ornithine decarboxylase ( OD), glyceraldehyde-3-phosphate dehydrogenase ( GAPDH) and glutathione S-transferase P1 ( GSP); two transcription factors, elongation factor 1 alpha and beta ( EF1α and EF1β); and one protein synthesis gene (ubiquitin ( UBQ). Primers specific for these genes were successfully developed for the three groups of oysters. Three different algorithms, geNorm, NormFinder and BestKeeper, were used to evaluate the expression stability of these candidate genes. BestKeeper program was found to be the most reliable. Based on our analysis, we found that the expression of RPL35 and EF1α was stable under low salinity stress, and the expression of OD, GAPDH and EF1α was stable under low temperature stress in hybrid (SA) oyster; the expression of RPS5 and GAPDH was stable under low salinity stress, and the expression of RPS5, UBQ, GAPDH was stable under low temperature stress in SS oyster; the expression of RPS5, GAPDH, EF1β and AA7 was stable under low salinity stress, and the expression of RPL35, EF1α, GAPDH

  9. Grain-size effects on PIXE and INAA analysis of IAEA-336 lichen reference material

    Science.gov (United States)

    Marques, A. P.; Freitas, M. C.; Wolterbeek, H. Th.; Verburg, T. G.; De Goeij, J. J. M.

    2007-02-01

    IAEA-336 lichen certified reference material was used to compare outcomes from INAA and PIXE elemental analyses, in relationship with grain size. The IAEA material (grain size lichen reference material's particle size distribution follows a bimodal distribution, which is turning more and more monomodal after further fine sieving. Replicates of each fraction were analysed by INAA and PIXE. Results for Cl, K, Mn, Fe and Zn by both techniques were compared by application of z-values tested against the criterion ∣ z∣ limited amount of lichen material as "seen" in the PIXE analysis and the grain size distribution in the lichen material were no causes of measurable differences between the results of both techniques. However, fractionation into smaller grain sizes showed to be associated with lower element content, for Na, Cl, K, Mn and Sr even up to a factor of 2. The observed increases of the proportion of algae in the smaller grain-size fractions and the possible accumulation capacity for certain elements in the fungal part of the lichen may explain the observed phenomenon. The sieving process and consequently the discarding of part of the material have lead to a change of the properties of the original sample, namely algae/fungus percentage and elemental contents.

  10. Contributions to reference systems from Lunar Laser Ranging using the IfE analysis model

    Science.gov (United States)

    Hofmann, Franz; Biskupek, Liliane; Müller, Jürgen

    2018-01-01

    Lunar Laser Ranging (LLR) provides various quantities related to reference frames like Earth orientation parameters, coordinates and velocities of ground stations in the Earth-fixed frame and selenocentric coordinates of the lunar retro-reflectors. This paper presents the recent results from LLR data analysis at the Institut für Erdmessung, Leibniz Universität Hannover, based on all LLR data up to the end of 2016. The estimates of long-periodic nutation coefficients with periods between 13.6 days and 18.6 years are obtained with an accuracy in the order of 0.05-0.7 milliarcseconds (mas). Estimations of the Earth rotation phase Δ UT are accurate at the level of 0.032 ms if more than 14 normal points per night are included. The tie between the dynamical ephemeris frame to the kinematic celestial frame is estimated from pure LLR observations by two angles and their rates with an accuracy of 0.25 and 0.02 mas per year. The estimated station coordinates and velocities are compared to the ITRF2014 solution and the geometry of the retro-reflector network with the DE430 solution. The given accuracies represent 3 times formal errors of the parameter fit. The accuracy for Δ UT is based on the standard deviation of the estimates with respect to the reference C04 solution.

  11. Optimization of instrumental neutron activation analysis for the within-bottle homogeneity study of reference materials of marine origin

    International Nuclear Information System (INIS)

    Silva, Daniel Pereira da

    2017-01-01

    The use of reference materials has been increasing in chemical analysis laboratories as its use is important for measurement validation in analytical chemistry. Such materials are generally imported, which require high financial investments in order to acquire them, and therefore it impacts on the difficulty to many national laboratories to use reference materials in their chemical analysis routine. Certification of reference materials is a complex process that assumes that the user is given appropriate assigned values of the properties of interests in the material. In this process, the homogeneity of the material must be checked. In this study, the within-bottle homogeneity study for the elements K, Mg, Mn, Na and V was performed for two reference materials of marine origin: the mussel reference material produced at the Neutron Activation Laboratory (LAN) of IPEN - CNEN/SP and an oyster tissue reference material produced abroad. For this purpose, the elements were determined in subsamples with masses varying between 1 and 250 mg by Instrumental Neutron Activation Analysis (INAA) and minimum sample intakes were estimated, ranging from 0.015 g for Na in the mussel reference material to 0.100 g for V in the two reference materials. (author)

  12. Analysis of data relative to the update of diagnostic reference levels in radiology and nuclear medicine. 2013-2015 review

    International Nuclear Information System (INIS)

    2016-11-01

    Applying the Order of 24 October 2011 on diagnostic reference levels, departments of radiology and nuclear medicine must send a sample of 'patient' dosimetric data to the IRSN each year. The results of the analysis of dosimetric data performed between the 1 January 2013 and the 31 December 2015 presented in this report should enable the authority to define the needs for updating regulation. This assessment takes place in a national and international context particularly rich and active since the last years. More than 20 years after the official introduction of the DRL concept by ICRP and the first regulation requirements at a European level, the good and the bad sides of the DRLs systems implemented by several countries, including France, has shown the necessity of complementary actions regarding some specific practices (pediatrics, interventional radiology). On one hand, from a national point of view, the current collection and analysis system is highly efficient for evaluation of practices in France and for DRL update ability. On the other hand, as an optimization implementation tool, regarding the lack of professionals involvement, the current system should not be considered as fully effective in radiology. However, when the professionals carry out DRL data collection and analysis, optimization actions are implemented for nearly all the cases. During the 2013-2015 period, professionals involvement in DRLs globally improved but is heterogeneous according to the imaging area considered. The participation of conventional radiology professionals is still low, with less than 30% against about 80% in CT and more than 85% in nuclear medicine. From a dosimetric point of view, the national analysis shows an overall decrease of statistical indicators in radiology, computed tomography and nuclear medicine on which DRLs are indexed. These results lead to proposals for updating reference values for a large number of examinations. In addition to the analysis of data

  13. Validation of reference genes for gene expression analysis in Valsa mali var. mali using real-time quantitative PCR.

    Science.gov (United States)

    Yin, Zhiyuan; Ke, Xiwang; Huang, Dingxuan; Gao, Xiaoning; Voegele, Ralf T; Kang, Zhensheng; Huang, Lili

    2013-09-01

    Valsa mali var. mali (Vmm), is the predominant species of apple valsa canker in China. Modern analysis of genes involved in virulence or pathogenicity usually implicate gene expression analysis most often performed using real-time quantitative polymerase chain reaction (RT-qPCR). However, for relative gene expression analysis pertinent reference genes have to be validated before using them as internal reference. This has not been reported for Vmm, so far. Therefore, eight commonly used housekeeping genes (ACT, CYP, EF1-α, G6PDH, GAPDH, L13, TUB, and UBQ) were cloned and evaluated for their expression stability by geNorm and NormFinder. Overall, all of the candidate reference genes were found to be suitable for gene expression analysis. After analysis of 10 samples from different strains and abiotic stress treatments, G6PDH appeared to be the most suitable reference gene, whereas GAPDH was the least suitable. Moreover, taking G6PDH combined with L13 or CYP as reference genes, improved the reliability of RT-qPCR significantly. The influence of the reference system on expression data was demonstrated by analyzing Vmmpg-1 encoding an endo-polygalacturonase gene. Pectinases are considered key pathogenicity factors for this fungus. In order to better understand the role of pectinases in pathogenicity of Vmm, RT-qPCR was used for expression analysis. Our results may provide a guideline for future studies on gene expression of V. mali var. mali by using RT-qPCR.

  14. SPM analysis of parametric (R)-[11C]PK11195 binding images: plasma input versus reference tissue parametric methods

    NARCIS (Netherlands)

    Schuitemaker, Alie; van Berckel, Bart N. M.; Kropholler, Marc A.; Veltman, Dick J.; Scheltens, Philip; Jonker, Cees; Lammertsma, Adriaan A.; Boellaard, Ronald

    2007-01-01

    (R)-[11C]PK11195 has been used for quantifying cerebral microglial activation in vivo. In previous studies, both plasma input and reference tissue methods have been used, usually in combination with a region of interest (ROI) approach. Definition of ROIs, however, can be labourious and prone to

  15. Evolutionary Analysis Predicts Sensitive Positions of MMP20 and Validates Newly- and Previously-Identified MMP20 Mutations Causing Amelogenesis Imperfecta

    Directory of Open Access Journals (Sweden)

    Barbara Gasse

    2017-06-01

    Full Text Available Amelogenesis imperfecta (AI designates a group of genetic diseases characterized by a large range of enamel disorders causing important social and health problems. These defects can result from mutations in enamel matrix proteins or protease encoding genes. A range of mutations in the enamel cleavage enzyme matrix metalloproteinase-20 gene (MMP20 produce enamel defects of varying severity. To address how various alterations produce a range of AI phenotypes, we performed a targeted analysis to find MMP20 mutations in French patients diagnosed with non-syndromic AI. Genomic DNA was isolated from saliva and MMP20 exons and exon-intron boundaries sequenced. We identified several homozygous or heterozygous mutations, putatively involved in the AI phenotypes. To validate missense mutations and predict sensitive positions in the MMP20 sequence, we evolutionarily compared 75 sequences extracted from the public databases using the Datamonkey webserver. These sequences were representative of mammalian lineages, covering more than 150 million years of evolution. This analysis allowed us to find 324 sensitive positions (out of the 483 MMP20 residues, pinpoint functionally important domains, and build an evolutionary chart of important conserved MMP20 regions. This is an efficient tool to identify new- and previously-identified mutations. We thus identified six functional MMP20 mutations in unrelated families, finding two novel mutated sites. The genotypes and phenotypes of these six mutations are described and compared. To date, 13 MMP20 mutations causing AI have been reported, making these genotypes and associated hypomature enamel phenotypes the most frequent in AI.

  16. Analysis of genetic diversity of southern Spain fig tree (Ficus carica L.) and reference materials as a tool for breeding and conservation.

    Science.gov (United States)

    Perez-Jiménez, M; López, B; Dorado, G; Pujadas-Salvá, A; Guzmán, G; Hernandez, P

    2012-06-01

    The common fig tree (Ficus carica L.) is a Mediterranean crop with problematic cultivar identification. The recovery and conservation of possible local varieties for ecological production requires the previous genetic characterization of the available germplasm. In this context, 42 lines corresponding to 12 local varieties and two caprifigs, in addition to 15 reference samples have been fingerprinted using 21 SSR markers. A total of 77 alleles were revealed, detecting a useful level of genetic variability within the local germplasm pools. UPGMA clustering analysis has revealed the genetic structure and relationships among the local and reference germplasm. Eleven of the local varieties could be identified and defined as obtained clusters, showing that SSR analysis is an efficient method to evaluate the Andalusian fig tree diversity for on-farm conservation. © 2012 The Authors.

  17. Reframing Student Affairs Leadership: An Analysis of Organizational Frames of Reference and Locus of Control

    Science.gov (United States)

    Tull, Ashley; Freeman, Jerrid P.

    2011-01-01

    Examined in this study were the identified frames of reference and locus of control used by 478 student affairs administrators. Administrator responses were examined to identify frames of reference most commonly used and their preference order. Locus of control most commonly used and the relationship between frames of reference and locus of…

  18. Analysis of the Questions Asked through Digital and Face-to-Face Reference Services

    Science.gov (United States)

    Tsuji, Keita; Arai, Shunsuke; Suga, Reina; Ikeuchi, Atsushi; Yoshikane, Fuyuki

    2013-01-01

    In Japan, only a few public libraries provide e-mail reference services. To help public libraries start e-mail reference services, the authors investigated reference questions received by libraries via e-mail and traditional face-to-face services. The authors found that research questions are more frequently observed among e-mail questions and…

  19. Antibody V and C domain sequence, structure, and interaction analysis with special reference to IMGT®.

    Science.gov (United States)

    Alamyar, Eltaf; Giudicelli, Véronique; Duroux, Patrice; Lefranc, Marie-Paule

    2014-01-01

    IMGT(®), the international ImMunoGeneTics information system(®) (http://www.imgt.org), created in 1989 (Centre National de la Recherche Scientifique, Montpellier University), is acknowledged as the global reference in immunogenetics and immunoinformatics. The accuracy and the consistency of the IMGT(®) data are based on IMGT-ONTOLOGY which bridges the gap between genes, sequences, and three-dimensional (3D) structures. Thus, receptors, chains, and domains are characterized with the same IMGT(®) rules and standards (IMGT standardized labels, IMGT gene and allele nomenclature, IMGT unique numbering, IMGT Collier de Perles), independently from the molecule type (genomic DNA, complementary DNA, transcript, or protein) or from the species. More particularly, IMGT(®) tools and databases provide a highly standardized analysis of the immunoglobulin (IG) or antibody and T cell receptor (TR) V and C domains. IMGT/V-QUEST analyzes the V domains of IG or TR rearranged nucleotide sequences, integrates the IMGT/JunctionAnalysis and IMGT/Automat tools, and provides IMGT Collier de Perles. IMGT/HighV-QUEST analyzes sequences from high-throughput sequencing (HTS) (up to 150,000 sequences per batch) and performs statistical analysis on up to 450,000 results, with the same resolution and high quality as IMGT/V-QUEST online. IMGT/DomainGapAlign analyzes amino acid sequences of V and C domains and IMGT/3Dstructure-DB and associated tools provide information on 3D structures, contact analysis, and paratope/epitope interactions. These IMGT(®) tools and databases, and the IMGT/mAb-DB interface with access to therapeutical antibody data, provide an invaluable help for antibody engineering and antibody humanization.

  20. Determination of Al, Si and P in certified reference materials by Instrumental Neutron Activation Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Noyori, Amanda; Saiki, Mitiko, E-mail: anoyori@gmail.com, E-mail: mitiko@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2017-11-01

    Neutron analysis activation is not commonly used for aluminum, phosphorus and silicon determination, due to the difficulty to obtain reliable results. In this study, Al was determined by measuring {sup 28}Al and the contribution of P and Si due to {sup 28}Al formed in {sup 31}P(n,α){sup 28}Al and {sup 28}Si(n,p){sup 28}Al reactions were corrected using correction factors determined experimentally. Phosphorus was determined by measuring {sup 32}P (pure beta emitter) formed in reaction {sup 31}P(n,γ){sup 32}P. Silicon was determined by epithermal neutron analysis activation (ENAA) and measuring {sup 29}Al radionuclide formed in {sup 29}Si(n,p){sup 29}Al reaction. Aliquots of certified reference materials (CRMs) and synthetic standards of the elements were irradiated together, using the pneumatic transfer station of IEA-R1 nuclear research reactor. Results obtained for biological and geological CRMs showed good precision and accuracy with |Z-score| < 2 for Al, P and Si determinations. The detection limits for Al, P and Si determinations CRMs were also evaluated. Results obtained in this study demonstrated the viability of applying INAA procedures in the determination of Al, P and Si. (author)

  1. Development of a Certified Reference Material (NMIJ CRM 7203-a) for Elemental Analysis of Tap Water.

    Science.gov (United States)

    Zhu, Yanbei; Narukawa, Tomohiro; Inagaki, Kazumi; Miyashita, Shin-Ichi; Kuroiwa, Takayoshi; Ariga, Tomoko; Kudo, Izumi; Koguchi, Masae; Heo, Sung Woo; Suh, Jung Ki; Lee, Kyoung-Seok; Yim, Yong-Hyeon; Lim, Youngran

    2017-01-01

    A certified reference material (CRM), NMIJ CRM 7203-a, was developed for the elemental analysis of tap water. At least two independent analytical methods were applied to characterize the certified value of each element. The elements certified in the present CRM were as follows: Al, As, B, Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, Pb, Rb, Sb, Se, Sr, and Zn. The certified value for each element was given as the (property value ± expanded uncertainty), with a coverage factor of 2 for the expanded uncertainty. The expanded uncertainties were estimated while considering the contribution of the analytical methods, the method-to-method variance, the sample homogeneity, the long-term stability, and the concentrations of the standard solutions for calibration. The concentration of Hg (0.39 μg kg -1 ) was given as the information value, since loss of Hg was observed when the sample was stored at room temperature and exposed to light. The certified values of selected elements were confirmed by a co-analysis carried out independently by the NMIJ (Japan) and the KRISS (Korea).

  2. Determination of 25 elements in biological standard reference materials by neutron activation analysis

    International Nuclear Information System (INIS)

    Guzzi, G.; Pietra, R.; Sabbioni, E.

    1974-12-01

    Standard and Certified Reference Materials programme of the JRC includes the determination of trace elements in complex biological samples delivered by the U.S. National Bureau of Standards: Bovine liver (NBS SRM 1577), Orchard Leaves (NBS SRM 1571) and Tomato Leaves. The study has been performed by the use of neutron activation analysis. Due to the very low concentration of some elements, radiochemical groups or elemental separation procedures were necessary. The paper describes the techniques used to analyse 25 elements. Computer assisted instrumental neutron activation analysis with high resolution Ge(Li) spectrometry was considerably advantageous in the determination of Na, K, Cl, Mn, Fe, Rb and Co and in some cases of Ca, Zn, Cs, Sc, and Cr. For low contents of Ca, Mg, Ni and Si special chemical separation schemes, followed by Cerenkov counting have been developped. Two other separation procedures allowing the determination of As, Cd, Ga, Hg, Mo, Cu, Sr Se, Ba and P have been set up. The first, the simplified one involves the use of high resolution Ge(Li) detectors, the second, the more complete one involves a larger number of shorter measurements performed by simpler and more sensitive techniques, such as NaI(Tl) scintillation spectrometry and Cerenkov counting. The results obtained are presented and discussed

  3. Determination of Al, Si and P in certified reference materials by Instrumental Neutron Activation Analysis

    International Nuclear Information System (INIS)

    Noyori, Amanda; Saiki, Mitiko

    2017-01-01

    Neutron analysis activation is not commonly used for aluminum, phosphorus and silicon determination, due to the difficulty to obtain reliable results. In this study, Al was determined by measuring 28 Al and the contribution of P and Si due to 28 Al formed in 31 P(n,α) 28 Al and 28 Si(n,p) 28 Al reactions were corrected using correction factors determined experimentally. Phosphorus was determined by measuring 32 P (pure beta emitter) formed in reaction 31 P(n,γ) 32 P. Silicon was determined by epithermal neutron analysis activation (ENAA) and measuring 29 Al radionuclide formed in 29 Si(n,p) 29 Al reaction. Aliquots of certified reference materials (CRMs) and synthetic standards of the elements were irradiated together, using the pneumatic transfer station of IEA-R1 nuclear research reactor. Results obtained for biological and geological CRMs showed good precision and accuracy with |Z-score| < 2 for Al, P and Si determinations. The detection limits for Al, P and Si determinations CRMs were also evaluated. Results obtained in this study demonstrated the viability of applying INAA procedures in the determination of Al, P and Si. (author)

  4. Neutronic analysis of a reference LEU core for Pakistan research reactor using oxide fuel

    International Nuclear Information System (INIS)

    Akhtar, K.M.; Qazi, M.K.; Bokhari, I.H.; Khan, L.A.; Pervez, S.

    1988-07-01

    Neutronic analysis of a 10 MW reference core for PARR, having 28 fresh LEU fuel elements arranged in a 6x5 configuration has been carried out using standard computer codes WIMS-D, EXTERMINATOR-II, and CITATION. Total nuclear power peaking of 3.2 has bee found to occur in the fuel plate adjacent to the water filled central flux trap at the depth of 43.8 cm from the top of the active core. Replacement of water in central flux trap with an aluminum block, having a 50 mm diameter water filled irradiation channel changes the flux profiles in fuel, core side flux trap and reflector. The thermal flux in the central flux trap decreases by about 53%. Therefore some of the fuel elements will have to be removed and the new configuration has to be analysed to determine the first operating core. However, after achieving some burn-up and confirmation from thermal hydraulic analysis, the core configuration analysed, will be the final working core. (orig./A.B.)

  5. Platinum stable isotope analysis of geological standard reference materials by double-spike MC-ICPMS.

    Science.gov (United States)

    Creech, J B; Baker, J A; Handler, M R; Bizzarro, M

    2014-01-10

    We report a method for the chemical purification of Pt from geological materials by ion-exchange chromatography for subsequent Pt stable isotope analysis by multiple-collector inductively coupled plasma mass spectrometry (MC-ICPMS) using a 196 Pt- 198 Pt double-spike to correct for instrumental mass bias. Double-spiking of samples was carried out prior to digestion and chemical separation to correct for any mass-dependent fractionation that may occur due to incomplete recovery of Pt. Samples were digested using a NiS fire assay method, which pre-concentrates Pt into a metallic bead that is readily dissolved in acid in preparation for anion-exchange chemistry. Pt was recovered from anion-exchange resin in concentrated HNO 3 acid after elution of matrix elements, including the other platinum group elements (PGE), in dilute HCl and HNO 3 acids. The separation method has been calibrated using a precious metal standard solution doped with a range of synthetic matrices and results in Pt yields of ≥90% with purity of ≥95%. Using this chemical separation technique, we have separated Pt from 11 international geological standard reference materials comprising of PGE ores, mantle rocks, igneous rocks and one sample from the Cretaceous-Paleogene boundary layer. Pt concentrations in these samples range from ca. 5 ng g -1 to 4 μg g -1 . This analytical method has been shown to have an external reproducibility on δ 198 Pt (permil difference in the 198 Pt/ 194 Pt ratio from the IRMM-010 standard) of ±0.040 (2 sd) on Pt solution standards (Creech et al., 2013, J. Anal. At. Spectrom. 28, 853-865). The reproducibility in natural samples is evaluated by processing multiple replicates of four standard reference materials, and is conservatively taken to be ca. ±0.088 (2 sd). Pt stable isotope data for the full set of reference materials have a range of δ 198 Pt values with offsets of up to 0.4‰ from the IRMM-010 standard, which are readily resolved with this technique. These

  6. Grain-size effects on PIXE and INAA analysis of IAEA-336 lichen reference material

    International Nuclear Information System (INIS)

    Marques, A.P.; Freitas, M.C.; Wolterbeek, H.Th.; Verburg, T.G.; Goeij, J.J.M. de

    2007-01-01

    IAEA-336 lichen certified reference material was used to compare outcomes from INAA and PIXE elemental analyses, in relationship with grain size. The IAEA material (grain size <125 μm) was ground and sieved through nylon nets with 64 μm, 41 μm and 20 μm pores. Particle sizes were determined by Laser Light Scattering technique: the data indicate that, after sieving, the IAEA-336 lichen reference material's particle size distribution follows a bimodal distribution, which is turning more and more monomodal after further fine sieving. Replicates of each fraction were analysed by INAA and PIXE. Results for Cl, K, Mn, Fe and Zn by both techniques were compared by application of z-values tested against the criterion vertical bar z vertical bar < 3 for approval of results at the 99.7% confidence level. Under the conditions of this study, the limited amount of lichen material as 'seen' in the PIXE analysis and the grain size distribution in the lichen material were no causes of measurable differences between the results of both techniques. However, fractionation into smaller grain sizes showed to be associated with lower element content, for Na, Cl, K, Mn and Sr even up to a factor of 2. The observed increases of the proportion of algae in the smaller grain-size fractions and the possible accumulation capacity for certain elements in the fungal part of the lichen may explain the observed phenomenon. The sieving process and consequently the discarding of part of the material have lead to a change of the properties of the original sample, namely algae/fungus percentage and elemental contents

  7. Grain-size effects on PIXE and INAA analysis of IAEA-336 lichen reference material

    Energy Technology Data Exchange (ETDEWEB)

    Marques, A.P. [ITN - Instituto Tecnologico e Nuclear, 2686-953 Sacavem (Portugal)]. E-mail: amarques@itn.pt; Freitas, M.C. [ITN - Instituto Tecnologico e Nuclear, 2686-953 Sacavem (Portugal); Wolterbeek, H.Th. [IRI - TU Delft, Mekelweg 15, 2629 JB Delft (Netherlands); Verburg, T.G. [IRI - TU Delft, Mekelweg 15, 2629 JB Delft (Netherlands); Goeij, J.J.M. de [IRI - TU Delft, Mekelweg 15, 2629 JB Delft (Netherlands)

    2007-02-15

    IAEA-336 lichen certified reference material was used to compare outcomes from INAA and PIXE elemental analyses, in relationship with grain size. The IAEA material (grain size <125 {mu}m) was ground and sieved through nylon nets with 64 {mu}m, 41 {mu}m and 20 {mu}m pores. Particle sizes were determined by Laser Light Scattering technique: the data indicate that, after sieving, the IAEA-336 lichen reference material's particle size distribution follows a bimodal distribution, which is turning more and more monomodal after further fine sieving. Replicates of each fraction were analysed by INAA and PIXE. Results for Cl, K, Mn, Fe and Zn by both techniques were compared by application of z-values tested against the criterion vertical bar z vertical bar < 3 for approval of results at the 99.7% confidence level. Under the conditions of this study, the limited amount of lichen material as 'seen' in the PIXE analysis and the grain size distribution in the lichen material were no causes of measurable differences between the results of both techniques. However, fractionation into smaller grain sizes showed to be associated with lower element content, for Na, Cl, K, Mn and Sr even up to a factor of 2. The observed increases of the proportion of algae in the smaller grain-size fractions and the possible accumulation capacity for certain elements in the fungal part of the lichen may explain the observed phenomenon. The sieving process and consequently the discarding of part of the material have lead to a change of the properties of the original sample, namely algae/fungus percentage and elemental contents.

  8. Safety and tolerability of frovatriptan in the acute treatment of migraine and prevention of menstrual migraine: Results of a new analysis of data from five previously published studies.

    Science.gov (United States)

    MacGregor, E Anne; Pawsey, Stephen P; Campbell, John C; Hu, Xiaojun

    2010-04-01

    Triptans are a recommended first-line treatment for moderate to severe migraine. Using clinical trial data, we evaluated the safety and tolerability of frovatriptan as acute treatment (AT) and as short-term preventive (STP) therapy for menstrual migraine (MM). Data from 2 Phase III AT trials (AT1: randomized, placebo controlled, 1 attack; AT2: 12-months, noncomparative, open label) and 3 Phase IIIb STP trials in MM (MMP1 and MMP2: randomized, placebo controlled, double blind, 3 perimenstrual periods; MMP3: open label, noncomparative, 12 perimenstrual periods) were analyzed. In AT1, patients treated each attack with frovatriptan 2.5 mg, sumatriptan 100 mg, or placebo. In AT2, they used frovatriptan 2.5 mg. In MMP1 and MMP2, women administered frovatriptan 2.5 mg for 6 days during the perimenstrual period, taking a loading dose of 2 or 4 tablets on day 1, followed by once-daily or BID frovatriptan 2.5 mg, respectively; in MMP3, they used BID frovatriptan 2.5 mg. In AT1, which was previously published in part, group differences in adverse events (AEs) were analyzed using the Fisher exact test, and response rates were compared using logistic regression. Post hoc analyses of sustained pain-free status with no AEs (SNAE) and sustained pain response with no AEs (SPRNAE) were performed using a 2-sample test for equality of proportions without continuity correction. For AT2 and the STP studies, data were summarized using descriptive statistics. Results of individual safety analyses for the STP studies were previously reported; the present report includes new results from a pooled analysis of MMP1 and MMP2 and a new analysis of MMP3 in which AEs were coded using Medical Dictionary for Regulatory Activities version 8.0. AT1 included 1206 patients in the safety group; AT2 included 496. In the STP studies, safety data were collected for 1487 women. In AT1 and AT2, 85.6% and 88.3%, respectively, of enrolled patients were women. Overall, AEs were generally mild to moderate (AT

  9. Common standards in facial esthetics: craniofacial analysis of most attractive black and white subjects according to People magazine during previous 10 years.

    Science.gov (United States)

    Iglesias-Linares, Alejandro; Yáñez-Vico, Rosa-María; Moreno-Manteca, Blanca; Moreno-Fernández, Ana María; Mendoza-Mendoza, Asunción; Solano-Reina, Enrique

    2011-06-01

    The aim of the present case-control study was to determine--using the 10 most recent annual listings of the most beautiful black and white people--whether any common, measurable esthetic parameter could be extracted from both groups. A total of 80 women (40 white and 40 black), who had been included in the list of People magazine's 100 most beautiful people during the previous 10 years, were selected. Lateral photographs of all 80 subjects were obtained from Internet databases, oriented, and sized. A modified photogrammetric analysis was performed on the lateral view of each subject to obtain the angle and proportion measurements. Differences between the 2 groups were compared with 95% significance using the Student t test for independent samples. Facial similarities in the 2 ethnic groups were observed for the angle of the inferior facial third, labiomental angle, angle of facial convexity, and cervicomental angle. These results point to a similar conformation of the lower part of the face in relation to the neck that was shared by both groups of beautiful women. Additionally, both groups showed similar results for the lower lip projection. Likewise, similar proportional measurements were found for the upper lip proportion, notwithstanding differences in protrusion. Modern society is changing the classic concept of facial beauty because of globalization and the prevalence of multiethnic communities in the developed world. Independently of ethnic origin, beautiful women tend to have similar facial features that are a mixture of both black and white features. Copyright © 2011 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.

  10. Quality assurance and reference material requirements and considerations for environmental sample analysis in nuclear forensics

    International Nuclear Information System (INIS)

    Swindle, D.W. Jr.; Perrin, R.E.; Goldberg, S.A.; Cappis, J.

    2002-01-01

    analyses are to be used in legal proceedings and prosecutions, and also in investigative research. From the legal perspective, one must assume that any results obtained would be used as evidence in a court of law for prosecution of criminal acts. Consequently, Quality Assurance requirements are very demanding and unforgiving. For example, forensics analysis results that cannot be independently verified against certified reference materials of known composition and isotopics, may not be accepted in a court of law as reliable evidence. Thus, the ability to provide certified and traceable reference materials used in the analyses are extremely important in nuclear forensics. In addition to nuclear signatures, other signatures such as any organics or plant and animal residue that are also on the intercepted materials, become important in identifying the origin of the intercepted nuclear material, and will require traceable, known standards. This paper will describe the quality assurance requirements and considerations that must be adopted and developed for application and use in nuclear forensics. Recommendations and minimum requirements for standardization of a nuclear forensics quality assurance program that could be internationally accepted will also be presented. (author)

  11. Analysis of urban particulate standard reference materials for the determination of chlorinated organic contaminants and additional chemical and physical properties

    Energy Technology Data Exchange (ETDEWEB)

    Poster, D.L.; Schantz, M.M.; Wise, S.A. [Analytical Chemistry Div., National Institute of Standards and Technology, Gaithersburg, MD (United States); Vangel, M.G. [Statistical Engineering Div., National Institute of Standards and Technology, Gaithersburg, MD (United States)

    1999-02-01

    A previously issued National Institute of Standards and Technology (NIST) Standard Reference Material (SRM), SRM 1649, Urban Dust/Organics has been analyzed for chlorinated organic contaminants (polychlorinated biphenyls and chlorinated pesticides) to provide certified values for a new class of compounds relative to the former certification. The material will be reissued as SRM 1649a. Four different analytical techniques were used. Specifically, two different methods of extraction (Soxhlet and pressurized fluid extraction) were used in conjunction with sample analysis by gas chromatography with two different columns (5% phenyl-methyl polysiloxane and 50% methyl C-18 dimethyl polysiloxane) that exhibit distinct selectivity, and with two different modes of detection (electron capture detection and mass spectrometry). The results from these techniques were combined to generate certified concentrations for 35 PCB congeners (some in combination) and 8 chlorinated pesticides. Ancillary assessments of additional chemical and physical properties of SRM 1649a include homogeneity, moisture, total organic carbon, extractable mass, and the particle-size distribution. The approach and the results for the certification of the PCB congeners and chlorinated pesticides in SRM 1649a, and the determination of the additional chemical and physical properties are described. In addition, the determination of PCBs and chlorinated pesticides in SRM 1648, Urban Particulate Matter (a particulate material certified for inorganic constituents), is also discussed although certified values are not presented. (orig.) With 6 figs., 4 tabs., 45 refs.

  12. Toxicity reference values for methylmercury effects on avian reproduction: Critical review and analysis.

    Science.gov (United States)

    Fuchsman, Phyllis C; Brown, Lauren E; Henning, Miranda H; Bock, Michael J; Magar, Victor S

    2017-02-01

    Effects of mercury (Hg) on birds have been studied extensively and with increasing frequency in recent years. The authors conducted a comprehensive review of methylmercury (MeHg) effects on bird reproduction, evaluating laboratory and field studies in which observed effects could be attributed primarily to Hg. The review focuses on exposures via diet and maternal transfer in which observed effects (or lack thereof) were reported relative to Hg concentrations in diet, eggs, or adult blood. Applicable data were identified for 23 species. From this data set, the authors identified ranges of toxicity reference values suitable for risk-assessment applications. Typical ranges of Hg effect thresholds are approximately 0.2 mg/kg to >1.4 mg/kg in diet, 0.05 mg/kg/d to 0.5 mg/kg/d on a dose basis, 0.6 mg/kg to 2.7 mg/kg in eggs, and 2.1 mg/kg to >6.7 mg/kg in parental blood (all concentrations on a wet wt basis). For Hg in avian blood, the review represents the first broad compilation of relevant toxicity data. For dietary exposures, the current data support TRVs that are greater than older, commonly used TRVs. The older diet-based TRVs incorporate conservative assumptions and uncertainty factors that are no longer justified, although they generally were appropriate when originally derived, because of past data limitations. The egg-based TRVs identified from the review are more similar to other previously derived TRVs but have been updated to incorporate new information from recent studies. While important research needs remain, a key recommendation is that species not yet tested for MeHg toxicity should be evaluated using toxicity data from tested species with similar body weights. Environ Toxicol Chem 2017;36:294-319. © 2016 SETAC. © 2016 SETAC.

  13. Reference Gene Selection for Gene Expression Analysis of Oocytes Collected from Dairy Cattle and Buffaloes during Winter and Summer

    Science.gov (United States)

    Gimenes, Lindsay Unno; de Carvalho, Nelcio Antonio Tonizza; Soares, Júlia Gleyci; Ayres, Henderson; Ferraz, Márcio Leão; Watanabe, Yeda Fumie; Watanabe, Osnir Yoshime; Sangalli, Juliano Rodrigues; Smith, Lawrence Charles; Baruselli, Pietro Sampaio; Meirelles, Flávio Vieira; Chiaratti, Marcos Roberto

    2014-01-01

    Oocytes from dairy cattle and buffaloes have severely compromised developmental competence during summer. While analysis of gene expression is a powerful technique for understanding the factors affecting developmental hindrance in oocytes, analysis by real-time reverse transcription PCR (RT-PCR) relies on the correct normalization by reference genes showing stable expression. Furthermore, several studies have found that genes commonly used as reference standards do not behave as expected depending on cell type and experimental design. Hence, it is recommended to evaluate expression stability of candidate reference genes for a specific experimental condition before employing them as internal controls. In acknowledgment of the importance of seasonal effects on oocyte gene expression, the aim of this study was to evaluate the stability of expression levels of ten well-known reference genes (ACTB, GAPDH, GUSB, HIST1H2AG, HPRT1, PPIA, RPL15, SDHA, TBP and YWHAZ) using oocytes collected from different categories of dairy cattle and buffaloes during winter and summer. A normalization factor was provided for cattle (RPL15, PPIA and GUSB) and buffaloes (YWHAZ, GUSB and GAPDH) based on the expression of the three most stable reference genes in each species. Normalization of non-reference target genes by these reference genes was shown to be considerably different from normalization by less stable reference genes, further highlighting the need for careful selection of internal controls. Therefore, due to the high variability of reference genes among experimental groups, we conclude that data normalized by internal controls can be misleading and should be compared to not normalized data or to data normalized by an external control in order to better interpret the biological relevance of gene expression analysis. PMID:24676354

  14. Levelized Cost of Energy Analysis of Marine and Hydrokinetic Reference Models: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Jenne, D. S.; Yu, Y. H.; Neary, V.

    2015-04-24

    In 2010 the U.S. Department of Energy initiated the development of six marine energy converter reference models. The reference models are point designs of well-known marine energy converters. Each device was designed to operate in a specific marine resource, instead of a generic device that can be deployed at any location. This method allows each device to be used as a benchmark for future reference model to benchmark future devices. The six designs consist of three current energy converters and three wave energy converters. The reference model project has generated both technical and economic data sets that are available in the public domain. The methodology to calculate the levelized cost of energy for the reference model project and an overall comparison of the cost of energy from these six reference-model designs are presented in this paper.

  15. Reference serving sizes for the Brazilian population: An analysis of processed food labels

    Directory of Open Access Journals (Sweden)

    Nathalie Kliemann

    2014-06-01

    Full Text Available OBJECTIVE: To compare serving sizes reported on processed food labels with reference serving sizes according to nutrition labeling legislation and the "Food Guide for the Brazilian Population". METHODS: This cross-sectional study analyzed the labels of 2,072 processed foods in a supermarket of Florianópolis, Santa Caratina, Brazil. The foods were classified according to the Brazilian food labeling legislation. Central tendency and variability values were calculated for the serving sizes and energy values reported on the labels, as well as the ratio between the reported and reference energy value. The Spearman correlation test was performed between the reference serving size and the reference energy density, and also between the reference serving size and energy density of each study food. RESULTS: Nutrition labeling and the Food Guide presented reference servings with different sizes and energy values. The serving sizes reported on the labels did not follow either of the references and presented heterogeneous values, with a maximum range of 55-240 g among ready and semi-ready pre-prepared dishes. The reported energy values were between 0.1 times smaller and 2.4 times larger than the reference values. The reference serving sizes presented a highly inverse correlation with the reference energy density (Spearman coefficient= 0.9 and a very low inverse correlation with the energy density of the foods analyzed (Spearman coefficient= 0.2. CONCLUSION: This study showed the need for standardizing reference serving size information for the Brazilian population as well as reviewing nutrition labeling legislation in order to standardize the serving sizes reported on labels and to update the reference energy density used to calculate serving sizes.

  16. The Reference Values for the Chair Stand Test in Healthy Japanese Older People: Determination by Meta-analysis.

    Science.gov (United States)

    Nakazono, Tetsuharu; Kamide, Naoto; Ando, Masataka

    2014-11-01

    [Purpose] The aim of this study was to determine the reference values for the chair stand test (CST) in healthy older Japanese people. [Methods] Relevant research articles for the 5-repetition chair stand test (CS-5) and the 30-second chair stand test (CS-30) were identified by electronic database and manual searching. Research articles involving healthy Japanese people aged 60 years and older were included in a meta-analysis. Weighted means of the CS-5 and CS-30 were estimated by the random effect model as the reference values for the CST. Further, the effects of age and sex on the reference values were analyzed by a meta-regression analysis. [Results] Seven articles (21 data) and three articles (14 data) were included in the meta-analyses for the CS-5 and CS-30, respectively. The reference value for the CS-5 was estimated as 8.50 sec [95% confidence interval (CI): 7.93-9.07]; age and sex were not associated with this reference value. The reference value for the CS-30 was estimated as 17.26 times [95%CI: 15.98-18.55], and age was significantly associated with this value. [Conclusion] When the CS-5 and CS-30 are used to evaluate elderly Japanese people, the reference values for the CS-5 and CS-30 determined in this study would be useful indices.

  17. Identification of appropriate reference genes for human mesenchymal stem cell analysis by quantitative real-time PCR.

    Science.gov (United States)

    Li, Xiuying; Yang, Qiwei; Bai, Jinping; Xuan, Yali; Wang, Yimin

    2015-01-01

    Normalization to a reference gene is the method of choice for quantitative reverse transcription-PCR (RT-qPCR) analysis. The stability of reference genes is critical for accurate experimental results and conclusions. We have evaluated the expression stability of eight commonly used reference genes found in four different human mesenchymal stem cells (MSC). Using geNorm, NormFinder and BestKeeper algorithms, we show that beta-2-microglobulin and peptidyl-prolylisomerase A were the optimal reference genes for normalizing RT-qPCR data obtained from MSC, whereas the TATA box binding protein was not suitable due to its extensive variability in expression. Our findings emphasize the significance of validating reference genes for qPCR analyses. We offer a short list of reference genes to use for normalization and recommend some commercially-available software programs as a rapid approach to validate reference genes. We also demonstrate that the two reference genes, β-actin and glyceraldehyde-3-phosphate dehydrogenase, are frequently used are not always successful in many cases.

  18. Extending the use of references to the literature: Lessons from a content analysis of mixed method case exemplars

    DEFF Research Database (Denmark)

    Creamer, Elizabeth G.; Musaeus, Peter; Edwards, Cherie

    2017-01-01

    The role of references to the literature in a scholarly publication is conceived principally in terms of its foundational role in establishing the need for a study and framing its contribution. This methodological inquiry used content analysis to examine how authors of mixed methods exemplars...... deployed references to the literature in the discussion section of their manuscripts. These authors also deployed references to literature in the discussion section to support statements about the implications and significance of the research or to explain unexpected findings. Immersion in a broad range...

  19. Previous Adverse Infant Outcomes as Predictors of Preconception Care Use: An Analysis of the 2010 and 2012 Los Angeles Mommy and Baby (LAMB) Surveys.

    Science.gov (United States)

    Batra, Priya; Higgins, Chandra; Chao, Shin M

    2016-06-01

    Objectives This study aimed to understand the impact of a previous adverse infant outcome (AIO) on use of preconception care prior to a subsequent pregnancy. Methods Responses from the 2010 and 2012 Los Angeles Mommy and Baby Surveys were analyzed. Weighted multivariate logistic regression was employed to identify significant associations between having had a previous AIO (preterm delivery, low birth weight infant, stillbirth, or major birth defect) and receipt of preconception care prior to the most recent pregnancy. Select patient-level covariates were included: chronic disease, age, education level, race/ethnicity, country of birth, insurance status prior to pregnancy and pregnancy intent. Adjustment for missing responses was performed using multiple chained imputation. Results After controlling for covariates, having had a previous AIO was associated with an increased odds of having utilized preconception care in the most recent pregnancy (OR 1.237, p = 0.040). Per the final regression model, a woman reporting a previous AIO and an intended subsequent pregnancy had a 42.4 % likelihood of having used preconception care. Of these women, only 28.8 % reported doing so because of concern regarding a previous birth complication. Discussion Women reporting a previous AIO were more likely to have used preconception care in a subsequent pregnancy. The prevalence of preconception care utilization remained low overall. Pregnancy intent emerged as a strong secondary predictor; any concerted strategy to improve access to preconception care must include initiatives to help ensure that pregnancies are planned.

  20. Trace elements determination in silicon and ferrosilicon reference materials by instrumental neutron activation analysis method

    International Nuclear Information System (INIS)

    Moreira, Edson Goncalves; Vasconcellos, Marina Beatriz Agostini; Saiki, Mitiko; Iamashita, Celia Omine

    2002-01-01

    The use of certified reference materials, CRM, is of uppermost importance in the rastreability realization of the measurement process. At times, CRM use is restricted by the non existence of a suitable CRM with similarity to the sample in respect to matrix composition or with element levels in different orders of magnitude. IPT Chemical Division launched a project to prepare a metallic silicon CRM, due to the requirements of the industries in this field. To characterize this new CRM, IPEN Nuclear Reactor Center is able to perform instrumental neutron activation analysis, INAA, a very suitable method for silicon matrix samples because they produce basically the short lived radionuclide 3 1 Si under thermal neutrons flux, which after radioactive decay, does not interfere in the determination of other elements. In this paper, it is presented the determination of As, Br, Co, Cr, K, Eu, Fe, La, Mn, Na Nb, Sb, Sm, Sc, Th, Tb, U, V, W and Yb in silicon CRM NBS SRM 57; ferrosilicon CRM IPT 56; IPT 70; NBS SRM 58a; NBS SRM 59a and silicon RM under preparation IPT 132. From the results, the accuracy and the precision of the process were assessed. (author)

  1. Inter-laboratory analysis of selected genetically modified plant reference materials with digital PCR.

    Science.gov (United States)

    Dobnik, David; Demšar, Tina; Huber, Ingrid; Gerdes, Lars; Broeders, Sylvia; Roosens, Nancy; Debode, Frederic; Berben, Gilbert; Žel, Jana

    2018-01-01

    Digital PCR (dPCR), as a new technology in the field of genetically modified (GM) organism (GMO) testing, enables determination of absolute target copy numbers. The purpose of our study was to test the transferability of methods designed for quantitative PCR (qPCR) to dPCR and to carry out an inter-laboratory comparison of the performance of two different dPCR platforms when determining the absolute GM copy numbers and GM copy number ratio in reference materials certified for GM content in mass fraction. Overall results in terms of measured GM% were within acceptable variation limits for both tested dPCR systems. However, the determined absolute copy numbers for individual genes or events showed higher variability between laboratories in one third of the cases, most possibly due to variability in the technical work, droplet size variability, and analysis of the raw data. GMO quantification with dPCR and qPCR was comparable. As methods originally designed for qPCR performed well in dPCR systems, already validated qPCR assays can most generally be used for dPCR technology with the purpose of GMO detection. Graphical abstract The output of three different PCR-based platforms was assessed in an inter-laboratory comparison.

  2. Analysis And Synthesis Of Model Reference Controller For Variable Speed Wind Generators Inertial Support

    Science.gov (United States)

    Bećirović, Elvisa; Osmić, Jakub; Kušljugić, Mirza; Perić, Nedjeljko

    2015-01-01

    Model Reference Controller (MRC) for contribution of Variable Speed Wind Generators (VSWG) in inertial response of Electrical Power System (EPS) is presented and analyzed in this paper. MRC is synthesized based on a model of Generating Unit With non-Reheat Steam Turbine (GUNRST) thus enabling VSWG to emulate GUNRST response during the initial stage of dynamic frequency response ie inertial phase. Very important property of conventional steam generating units is that its contribution to inertial phase response is independent from the initial generating power. By using MRC in VSWG it is accomplished that in most common wind speed region (3-12 m/s) VSWG inertial support is almost independent from wind speed. Since in most EPSs VSWG replaces conventional steam generators, application of MRC algorithm provides that the characteristics of EPS in terms of inertial response are preserved, regardless of the growing trend of introducing VSWG. Evaluation analysis of the proposed MRC is performed on modified nine bus power system when VSWG with MRC is connected to one of the power system buses.

  3. Determination of trace elements in food reference materials by instrumental neutron activation analysis

    International Nuclear Information System (INIS)

    Cho, K. H.; Park, K. W.; Zeisler, R.

    2005-01-01

    Two biological Certified Reference Materials (CRMs), KRISS 108-04-001 (ouster tissue) and 108-05-001 (water dropwort stem), were prepared by Korea Research Institute of Standards and Science (KRISS) during FY '01. The certified values of these materials had been determined by Isotope Dilution Mass Spectrometry (IDMS) for six elements (Cd, Cr, Cu, Fe, Pb and Zn). Additional analytical works are now progressing to certify the concentrations of a number of the environmental and nutrimental elements in these CRMs. The certified values in a CRM are usually determined by using a single primary method with confirmation by other method(s) or using two independent critically-evaluated methods. Instrumental Neutron Activation Analysis (INAA) plays an important role in the determination of certified values as it can eliminate the possibility of common error sources resulting from sample dissolution. In this study INAA procedure was used in determination of 23 elements in these two biological CRMs to acquire the concentration information and the results were compared with KRISS certified values

  4. Certified sediment reference materials for trace element analysis from the National Metrology Institute of Japan (NMIJ).

    Science.gov (United States)

    Inagaki, Kazumi; Takatsu, Akiko; Kuroiwa, Takayoshi; Nakama, Atsuko; Eyama, Sakae; Chiba, Koichi; Okamoto, Kensaku

    2004-03-01

    Two types of sediment reference material (NMIJ 7302-a and 7303-a) for trace elements analysis have been prepared and certified by the National Metrology Institute of Japan in the National Institute of Advanced Industrial Science and Technology (NMIJ/AIST). The original materials were collected from a bay near industrial activity in Kyushu (NMIJ CRM 7302-a; marine sediment) and from Lake Biwa (NMIJ CRM 7303-a; lake sediment). The sediment materials were air-dried, sieved, homogenized, packaged in 1000 glass bottles (60 g each), and radiation sterilized. Certification of these CRM for trace elements was conducted by NMIJ, where each element was determined by at least two independent analytical techniques. Isotope-dilution inductively coupled plasma mass spectrometry (ICP-MS) was applied for certification of all the elements except mono-nuclide elements such as As and Co. Other techniques such as ICP-MS with quadrupole mass spectrometry and sector-field mass spectrometry, inductively coupled plasma atomic emission spectrometry (ICP-AES), and atomic absorption spectrometry (AAS), were also used. Certified values have been provided for 14 elements (Sb, As, Cd, Cr, Co, Cu, Pb, Hg, Mo, Ni, Se, Ag, Sn, and Zn) in both CRM.

  5. Development and application of a multi-targeting reference plasmid as calibrator for analysis of five genetically modified soybean events.

    Science.gov (United States)

    Pi, Liqun; Li, Xiang; Cao, Yiwei; Wang, Canhua; Pan, Liangwen; Yang, Litao

    2015-04-01

    Reference materials are important in accurate analysis of genetically modified organism (GMO) contents in food/feeds, and development of novel reference plasmid is a new trend in the research of GMO reference materials. Herein, we constructed a novel multi-targeting plasmid, pSOY, which contained seven event-specific sequences of five GM soybeans (MON89788-5', A2704-12-3', A5547-127-3', DP356043-5', DP305423-3', A2704-12-5', and A5547-127-5') and sequence of soybean endogenous reference gene Lectin. We evaluated the specificity, limit of detection and quantification, and applicability of pSOY in both qualitative and quantitative PCR analyses. The limit of detection (LOD) was as low as 20 copies in qualitative PCR, and the limit of quantification (LOQ) in quantitative PCR was 10 copies. In quantitative real-time PCR analysis, the PCR efficiencies of all event-specific and Lectin assays were higher than 90%, and the squared regression coefficients (R(2)) were more than 0.999. The quantification bias varied from 0.21% to 19.29%, and the relative standard deviations were from 1.08% to 9.84% in simulated samples analysis. All the results demonstrated that the developed multi-targeting plasmid, pSOY, was a credible substitute of matrix reference materials, and could be used as a reliable reference calibrator in the identification and quantification of multiple GM soybean events.

  6. Clinical evaluation of recombinant factor VIII preparation (Kogenate) in previously treated patients with hemophilia A: descriptive meta-analysis of post-marketing study data.

    Science.gov (United States)

    Yoshioka, A; Fukutake, K; Takamatsu, J; Shirahata, A

    2006-08-01

    The safety and efficacy of Kogenate, a recombinant factor VIII (rFVIII) preparation for the treatment of bleeding episodes, were studied in a 123-patient meta-analysis population of previously treated patients (PTPs), including 15 enrolled in the registration Phase III trial (PTP-I group), 93 from the post-marketing special investigation (PTP-II group), and 15 from short-term special investigations in surgery or tooth extraction (SI group). These patients (82 severe, 31 moderate, 9 mild, and 1 unknown), aged 11 months to 72 years, were enrolled in 28 centers in Japan. Blood samples taken at the baseline and at 3, 6, 9, 12, 18, and 24 months after the introduction of Kogenate were evaluated for FVIII inhibitor antibodies, antibodies formed against trace proteins derived from the rFVIII production process, and for general changes in laboratory test results. Mean exposure to Kogenate was 1103 days in PTP-I, 86 days in PTP-II, 27 days in patients in surgery, and 2 days in patients with tooth extraction. Assessment of FVIII inhibitor activity was conducted in 115 of the 123 patients by means of the Bethesda assay. Twelve patients were found to have a low titer of FVIII inhibitor (0.5-3.0 BU/mL) prior to any administration of Kogenate, and 103 were inhibitor-negative at the baseline. Among this latter group, 3 patients (2.9%) tested inhibitor-positive, with titers ranging from 1.2 to 2.1 BU/mL, with 4 patients below 1.0 BU/mL. One patient in the 11 PTPs investigated (PTP-I) developed antibodies against baby hamster kidney protein and mouse immunoglobulin G, but these findings were transient and asymptomatic. Hemostasis was achieved (markedly effective or effective) in 3666 of the 3855 bleeding episodes (95.1%) observed in 108 patients. Only 1 infusion was necessary in 3790 (98.3%) of these episodes. These data indicate that Kogenate is safe and very effective for the treatment of bleeding in PTPs with hemophilia A.

  7. Final safety analysis report for the Galileo Mission: Volume 1, Reference design document

    Energy Technology Data Exchange (ETDEWEB)

    1988-05-01

    The Galileo mission uses nuclear power sources called Radioisotope Thermoelectric Generators (RTGs) to provide the spacecraft's primary electrical power. Because these generators contain nuclear material, a Safety Analysis Report (SAR) is required. A preliminary SAR and an updated SAR were previously issued that provided an evolving status report on the safety analysis. As a result of the Challenger accident, the launch dates for both Galileo and Ulysses missions were later rescheduled for November 1989 and October 1990, respectively. The decision was made by agreement between the DOE and the NASA to have a revised safety evaluation and report (FSAR) prepared on the basis of these revised vehicle accidents and environments. The results of this latest revised safety evaluation are presented in this document (Galileo FSAR). Volume I, this document, provides the background design information required to understand the analyses presented in Volumes II and III. It contains descriptions of the RTGs, the Galileo spacecraft, the Space Shuttle, the Inertial Upper Stage (IUS), the trajectory and flight characteristics including flight contingency modes, and the launch site. There are two appendices in Volume I which provide detailed material properties for the RTG.

  8. Reference materials (RMs) for analysis of the human factor II (prothrombin) gene G20210A mutation

    NARCIS (Netherlands)

    Klein, Christoph L.; Márki-Zay, János; Corbisier, Philippe; Gancberg, David; Cooper, Susan; Gemmati, Donato; Halbmayer, Walter-Michael; Kitchen, Steve; Melegh, Béla; Neumaier, Michael; Oldenburg, Johannes; Leibundgut, Elisabeth Oppliger; Reitsma, Pieter H.; Rieger, Sandra; Schimmel, Heinz G.; Spannagl, Michael; Tordai, Attilia; Tosetto, Alberto; Visvikis, Sophie; Zadro, Renata; Mannhalter, Christine

    2005-01-01

    The Scientific Committee of Molecular Biology Techniques (C-MbT) in Clinical Chemistry of the IFCC has initiated a joint project in co-operation with the European Commission, Joint Research Centre, Institute of Reference Materials and Measurements to develop and produce plasmid-type reference

  9. Contemporary Development of Academic Reference Librarianship in the United States: A 44-Year Content Analysis

    Science.gov (United States)

    Wang, Hanrong; Tang, Yingqi; Knight, Carley

    2010-01-01

    This study analyzes job advertisements to identify the contemporary development of academic reference librarianship in the United States. Results show that more job openings, higher educational backgrounds, more duties & responsibilities, and variety of titles were assigned to academic reference librarian positions from 1966 through 2009.…

  10. Food references and marketing to children in Australian magazines: a content analysis.

    Science.gov (United States)

    Kelly, Bridget; Chapman, Kathy

    2007-12-01

    The aim of this study was to assess the content and extent of food references and marketing within popular children's magazines in Australia. Sixteen popular Australian children's magazines were selected, as determined by readership and circulation data. Back copies of each magazine were purchased for publications released between January and December 2006 (n = 76). Each magazine was assessed for food references on the basis of 23 food categories and 7 food-referencing types and as either branded or non-branded food references. There were a high number of overall food references within the children's magazines, with the majority of these being for unhealthy food products (63.7% unhealthy versus 36.3% healthy foods, p marketing, were ice cream and iced confection (85.6% branded references), fast food restaurant meals (83.4%), high-sugar drinks (78.9%) and snack foods (73.4%). Of all magazines, those targeting males and children aged 7-12 years had the highest proportion of unhealthy food references (78.1 and 69.8% unhealthy food references, respectively). Food references within children's magazines are common and skewed towards unhealthy foods. Children's high magazine readership rates and a lack of advertising and product placement regulations for magazines in Australia make this media an attractive target for food marketers. The timely establishment of food marketing regulations within magazines are recommended to prevent further expansion of food marketing in this area.

  11. Reference List Accuracy in Social Work Journals: A Follow-Up Analysis

    Science.gov (United States)

    Mitchell-Williams, Missy T.; Skipper, Antonius D.; Alexander, Marvin C.; Wilks, Scott E.

    2017-01-01

    Purpose: Following up an "Research on Social Work Practice" article published a decade ago, this study aimed to examine reference error rates among five, widely circulated social work journals. Methods: A stratified random sample of references was selected from the year 2013 (N = 500, 100/journal). Each was verified against the original…

  12. Reference Values for Knee Extension Strength Obtained by Hand-Held Dynamometry from Apparently Healthy Older Adults: A Meta-Analysis.

    Science.gov (United States)

    Bohannon, R W

    2017-01-01

    Normative reference values for knee extension strength obtained by hand-held dynamometry from adults 60 to 79 years were derived using summary data from 3 studies. The studies, which were identified through PubMed and a hand-search, contributed data from between 3 and 29 individuals for each age, gender, and side stratum. Meta-analysis was employed to consolidate knee extension strength data. Strength, normalized against body weight, ranged 35.6% for the nondominant side of 70 to 79 year old women to 48.8% for the nondominant side of 60 to 69 year old men. These values are more informative than those previously published in individual studies. However, reference values derived from a large population-based sample are needed.

  13. Fine-mapping analysis of the MHC region for vitiligo based on a new Han-MHC reference panel.

    Science.gov (United States)

    Yang, Chao; Wu, Juan; Zhang, Xuelei; Wen, Leilei; Sun, Jingying; Cheng, Yuyan; Tang, Xianfa; Liang, Bo; Chen, Gang; Zhou, Fusheng; Cui, Yong; Zhang, Anping; Zhang, Xuejun; Zheng, Xiaodong; Yang, Sen; Sun, Liangdan

    2018-03-30

    Vitiligo is an immune-related disease with patchy depigmentation of skin and hair caused by selective destruction of melanocytes. In recent decades, many studies have shown the association between vitiligo and HLA genes; however, the results of Han Chinese are scarce. In this study, we performed a fine-mapping analysis of the MHC region in 2818 Han Chinese subjects through a widely used HLA imputation method with a newly built large-scale Han-MHC reference panel. Three new four-digit HLA alleles (HLA-DQB1 ∗ 02:02, HLA-DQA1 ∗ 02:01 and HLA-DPB1 ∗ 17:01) were identified to be associated with the risk of vitiligo, and four previously reported alleles were confirmed. Further conditional analysis revealed that two important variants, HLA-DQβ1 amino acid position 135 (OR = 1.79, P = 1.87 × 10 -11 ) and HLA-B amino acid positions 45-46 (OR = 1.44, P = 5.61 × 10 -11 ), conferred most of the MHC associations. Three-dimension ribbon models showed that the former is located within the β2 domain of the HLA-DQβ1 molecule, and the latter lies in the α1 domain of the HLA-B molecule, while both are involved in specific antigen presenting process. Finally, we summarized all significant signals in the MHC region to clarify their complex relationships, and 8.60% of phenotypic variance could be explained based on all reported variants in Han Chinese so far. Our findings highlight the complex genetic architecture of the MHC region for vitiligo in Han Chinese population and expand our understanding of the roles of HLA coding variants in the etiology of vitiligo. Copyright © 2018. Published by Elsevier B.V.

  14. Getting Started With Electronic Reference Statistics: Case Studies and Best Practices for Collection and Analysis

    Directory of Open Access Journals (Sweden)

    Elaine H. Dean

    2013-10-01

    Full Text Available Collecting reference statistics is an important facet of academic librarianship. Having accurate, shareable data about the services a library provides is key to understanding the needs of users and highlighting the importance of the library within the overall institution. While many libraries collect reference statistics on paper, gathering this data using an electronic statistics system is an efficient and customizable way to track and analyze reference service trends. By detailing Neumann University Library and Harrell Health Sciences Library’s experiences with two different but equally effective systems, this article will explore the complexities of implementing, customizing, and analyzing the data from an electronic reference statistics system. The authors also discuss challenges encountered and offer recommendations and best practices for others wishing to explore electronic reference statistic collection.

  15. Meta-analysis of the accuracy of tools used for binary classification when the primary studies employ different references.

    Science.gov (United States)

    Botella, Juan; Huang, Huiling; Suero, Manuel

    2015-09-01

    The quality of tools used in binary classification is evaluated by studies that assess the accuracy of the classification. The empirical evidence is summarized in 2 × 2 contingency tables. These provide the joint frequencies between the true status of a sample and the classification made by the test. The accuracy of the test is better estimated in a meta-analysis that synthesizes the results of a set of primary studies. The true status is determined by a reference that ideally is a gold standard, which means that it is error free. However, in psychology, it is rare that all the primary studies have employed the same reference, and often they have used an imperfect reference with suboptimal accuracy instead of an actual gold standard. An imperfect reference biases both the estimates of the accuracy of the test and the empirical prevalence of the target status in the primary studies. We discuss several strategies for meta-analysis when different references are employed. Special attention is paid to the simplest case, where the meta-analyst has 1 group of primary studies using a reference that can be considered a gold standard and a 2nd group of primary studies using an imperfect reference. A procedure is recommended in which the frequencies from the primary studies with the imperfect reference are corrected prior to the meta-analysis itself. Then, a hierarchical meta-analytic model is fitted. An example with actual data from SCOFF (Sick-Control-One-Fat-Food; Hill, Reid, Morgan, & Lacey, 2010; Morgan, Reid, & Lacey, 1999) a simple but efficient test for detecting eating disorders, is described. (c) 2015 APA, all rights reserved).

  16. Robust RT-qPCR data normalization: validation and selection of internal reference genes during post-experimental data analysis.

    Directory of Open Access Journals (Sweden)

    Daijun Ling

    Full Text Available Reverse transcription and real-time PCR (RT-qPCR has been widely used for rapid quantification of relative gene expression. To offset technical confounding variations, stably-expressed internal reference genes are measured simultaneously along with target genes for data normalization. Statistic methods have been developed for reference validation; however normalization of RT-qPCR data still remains arbitrary due to pre-experimental determination of particular reference genes. To establish a method for determination of the most stable normalizing factor (NF across samples for robust data normalization, we measured the expression of 20 candidate reference genes and 7 target genes in 15 Drosophila head cDNA samples using RT-qPCR. The 20 reference genes exhibit sample-specific variation in their expression stability. Unexpectedly the NF variation across samples does not exhibit a continuous decrease with pairwise inclusion of more reference genes, suggesting that either too few or too many reference genes may detriment the robustness of data normalization. The optimal number of reference genes predicted by the minimal and most stable NF variation differs greatly from 1 to more than 10 based on particular sample sets. We also found that GstD1, InR and Hsp70 expression exhibits an age-dependent increase in fly heads; however their relative expression levels are significantly affected by NF using different numbers of reference genes. Due to highly dependent on actual data, RT-qPCR reference genes thus have to be validated and selected at post-experimental data analysis stage rather than by pre-experimental determination.

  17. The utility of optical detection system (qPCR) and bioinformatics methods in reference gene expression analysis

    Science.gov (United States)

    Skarzyńska, Agnieszka; Pawełkowicz, Magdalena; PlÄ der, Wojciech; Przybecki, Zbigniew

    2016-09-01

    Real-time quantitative polymerase chain reaction is consider as the most reliable method for gene expression studies. However, the expression of target gene could be misinterpreted due to improper normalization. Therefore, the crucial step for analysing of qPCR data is selection of suitable reference genes, which should be validated experimentally. In order to choice the gene with stable expression in the designed experiment, we performed reference gene expression analysis. In this study genes described in the literature and novel genes predicted as control genes, based on the in silico analysis of transcriptome data were used. Analysis with geNorm and NormFinder algorithms allow to create the ranking of candidate genes and indicate the best reference for flower morphogenesis study. According to the results, genes CACS and CYCL were characterised the most stable expression, but the least suitable genes were TUA and EF.

  18. An analysis of 97 previously diagnosed de novo adult acute erythroid leukemia patients following the 2016 revision to World Health Organization classification.

    Science.gov (United States)

    Qiu, Shaowei; Jiang, Erlie; Wei, Hui; Lin, Dong; Zhang, Guangji; Wei, Shuning; Zhou, Chunlin; Liu, Kaiqi; Wang, Ying; Liu, Bingcheng; Liu, Yuntao; Gong, Benfa; Gong, Xiaoyuan; Feng, Sizhou; Mi, Yingchang; Han, Mingzhe; Wang, Jianxiang

    2017-08-09

    The incidence of acute erythroid leukemia subtype (AEL) is rare, accounting for 5% of cases of acute myeloid leukemia (AML), and the outcome is dismal. However, in 2016 revision to the WHO classification, the subcategory of AEL has been removed. Myeloblasts are redefined as the percentage of total marrow cells, not non-erythroid cells. Therefore, the previously diagnosed AEL cases are currently diagnosed as AML or myelodyspalstic syndrome (MDS) according to new criteria. We respectively reviewed cases of 97 de novo previously diagnosed AEL and all the patients were diagnosed as AML or MDS according to the new classification scheme, and then the clinical characteristics of these two subtypes were compared. Statistical analyses were performed by SPSS software version 18.0. The median age was 37 years-old, the two-thirds of previous AEL cases were diagnosed as MDS, and there was no obvious difference between two subtypes except for male/female ratio and age. Cytogenetic, rather than MDS/AML subtypes, can better represent the prognostic factor of previously diagnosed AEL patients. When the cytogenetic risk of patients belonged to MRC intermediate category and age were below 40 years-old in previous AEL cases, the patients who received induction chemotherapy without transplantation had a similar survival compared with the patients who underwent transplantation (3-year OS: 67.2% vs 68.5%). Cytogenetic, rather than MDS/AML subtypes, can better represent the prognostic factor of previously diagnosed AEL patients. Transplantation was a better choice for those whose cytogenetic category was unfavorable.

  19. Functional classification of genes using semantic distance and fuzzy clustering approach: evaluation with reference sets and overlap analysis.

    Science.gov (United States)

    Devignes, Marie-Dominique; Benabderrahmane, Sidahmed; Smaïl-Tabbone, Malika; Napoli, Amedeo; Poch, Olivier

    2012-01-01

    Functional classification aims at grouping genes according to their molecular function or the biological process they participate in. Evaluating the validity of such unsupervised gene classification remains a challenge given the variety of distance measures and classification algorithms that can be used. We evaluate here functional classification of genes with the help of reference sets: KEGG (Kyoto Encyclopaedia of Genes and Genomes) pathways and Pfam clans. These sets represent ground truth for any distance based on GO (Gene Ontology) biological process and molecular function annotations respectively. Overlaps between clusters and reference sets are estimated by the F-score method. We test our previously described IntelliGO semantic distance with hierarchical and fuzzy C-means clustering and we compare results with the state-of-the-art DAVID (Database for Annotation Visualisation and Integrated Discovery) functional classification method. Finally, study of best matching clusters to reference sets leads us to propose a set-difference method for discovering missing information.

  20. Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) Code Reference Manual

    International Nuclear Information System (INIS)

    C. L. Smith; K. J. Kvarfordt; S. T. Wood

    2006-01-01

    The Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) is a software application developed for performing a complete probabilistic risk assessment (PRA) using a personal computer. SAPHIRE is funded by the U.S. Nuclear Regulatory Commission (NRC) and developed by the Idaho National Laboratory (INL). The INL's primary role in this project is that of software developer. However, the INL also plays an important role in technology transfer by interfacing and supporting SAPHIRE users comprised of a wide range of PRA practitioners from the NRC, national laboratories, the private sector, and foreign countries. SAPHIRE can be used to model a complex system's response to initiating events, quantify associated damage outcome frequencies, and identify important contributors to this damage (Level 1 PRA) and to analyze containment performance during a severe accident and quantify radioactive releases (Level 2 PRA). It can be used for a PRA evaluating a variety of operating conditions, for example, for a nuclear reactor at full power, low power, or at shutdown conditions. Furthermore, SAPHIRE can be used to analyze both internal and external initiating events and has special features for ansforming models built for internal event analysis to models for external event analysis. It can also be used in a limited manner to quantify risk in terms of release consequences to both the public and the environment (Level 3 PRA). SAPHIRE includes a separate module called the Graphical Evaluation Module (GEM). GEM provides a highly specialized user interface with SAPHIRE that automates SAPHIRE process steps for evaluating operational events at commercial nuclear power plants. Using GEM, an analyst can estimate the risk associated with operational events in a very efficient and expeditious manner. This reference guide will introduce the SAPHIRE Version 7.0 software. A brief discussion of the purpose and history of the software is included along with general

  1. Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) Code Reference Manual

    Energy Technology Data Exchange (ETDEWEB)

    C. L. Smith; K. J. Kvarfordt; S. T. Wood

    2008-08-01

    The Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) is a software application developed for performing a complete probabilistic risk assessment (PRA) using a personal computer. SAPHIRE is funded by the U.S. Nuclear Regulatory Commission (NRC) and developed by the Idaho National Laboratory (INL). The INL's primary role in this project is that of software developer. However, the INL also plays an important role in technology transfer by interfacing and supporting SAPHIRE users comprised of a wide range of PRA practitioners from the NRC, national laboratories, the private sector, and foreign countries. SAPHIRE can be used to model a complex system’s response to initiating events, quantify associated damage outcome frequencies, and identify important contributors to this damage (Level 1 PRA) and to analyze containment performance during a severe accident and quantify radioactive releases (Level 2 PRA). It can be used for a PRA evaluating a variety of operating conditions, for example, for a nuclear reactor at full power, low power, or at shutdown conditions. Furthermore, SAPHIRE can be used to analyze both internal and external initiating events and has special features for transforming models built for internal event analysis to models for external event analysis. It can also be used in a limited manner to quantify risk in terms of release consequences to both the public and the environment (Level 3 PRA). SAPHIRE includes a separate module called the Graphical Evaluation Module (GEM). GEM provides a highly specialized user interface with SAPHIRE that automates SAPHIRE process steps for evaluating operational events at commercial nuclear power plants. Using GEM, an analyst can estimate the risk associated with operational events in a very efficient and expeditious manner. This reference guide will introduce the SAPHIRE Version 7.0 software. A brief discussion of the purpose and history of the software is included along with

  2. Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) Code Reference Manual

    Energy Technology Data Exchange (ETDEWEB)

    C. L. Smith; K. J. Kvarfordt; S. T. Wood

    2006-07-01

    The Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) is a software application developed for performing a complete probabilistic risk assessment (PRA) using a personal computer. SAPHIRE is funded by the U.S. Nuclear Regulatory Commission (NRC) and developed by the Idaho National Laboratory (INL). The INL's primary role in this project is that of software developer. However, the INL also plays an important role in technology transfer by interfacing and supporting SAPHIRE users comprised of a wide range of PRA practitioners from the NRC, national laboratories, the private sector, and foreign countries. SAPHIRE can be used to model a complex system’s response to initiating events, quantify associated damage outcome frequencies, and identify important contributors to this damage (Level 1 PRA) and to analyze containment performance during a severe accident and quantify radioactive releases (Level 2 PRA). It can be used for a PRA evaluating a variety of operating conditions, for example, for a nuclear reactor at full power, low power, or at shutdown conditions. Furthermore, SAPHIRE can be used to analyze both internal and external initiating events and has special features for ansforming models built for internal event analysis to models for external event analysis. It can also be used in a limited manner to quantify risk in terms of release consequences to both the public and the environment (Level 3 PRA). SAPHIRE includes a separate module called the Graphical Evaluation Module (GEM). GEM provides a highly specialized user interface with SAPHIRE that automates SAPHIRE process steps for evaluating operational events at commercial nuclear power plants. Using GEM, an analyst can estimate the risk associated with operational events in a very efficient and expeditious manner. This reference guide will introduce the SAPHIRE Version 7.0 software. A brief discussion of the purpose and history of the software is included along with

  3. Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samples

    Directory of Open Access Journals (Sweden)

    Afendy Arian

    2010-05-01

    Full Text Available Abstract Background Given the epidemic proportions of obesity worldwide and the concurrent prevalence of metabolic syndrome, there is an urgent need for better understanding the underlying mechanisms of metabolic syndrome, in particular, the gene expression differences which may participate in obesity, insulin resistance and the associated series of chronic liver conditions. Real-time PCR (qRT-PCR is the standard method for studying changes in relative gene expression in different tissues and experimental conditions. However, variations in amount of starting material, enzymatic efficiency and presence of inhibitors can lead to quantification errors. Hence the need for accurate data normalization is vital. Among several known strategies for data normalization, the use of reference genes as an internal control is the most common approach. Recent studies have shown that both obesity and presence of insulin resistance influence an expression of commonly used reference genes in omental fat. In this study we validated candidate reference genes suitable for qRT-PCR profiling experiments using visceral adipose samples from obese and lean individuals. Results Cross-validation of expression stability of eight selected reference genes using three popular algorithms, GeNorm, NormFinder and BestKeeper found ACTB and RPII as most stable reference genes. Conclusions We recommend ACTB and RPII as stable reference genes most suitable for gene expression studies of human visceral adipose tissue. The use of these genes as a reference pair may further enhance the robustness of qRT-PCR in this model system.

  4. Sonographic Measurement of Lower Uterine Segment Thickness to Predict Uterine Rupture During a Trial of Labor in Women With Previous Cesarean Section: A Meta-analysis

    NARCIS (Netherlands)

    Kok, N.; Wiersma, I. C.; Opmeer, B. C.; de Graaf, I. M.; Mol, B. W.; Pajkrt, E.

    2014-01-01

    Along with the increasing rate of cesarean section (CS) births has been a concomitant decrease in the rate of vaginal birth after cesarean (VBAC), mostly due to concerns about uterine rupture during a trial of labor (TOL). The risk of uterine rupture in laboring women with a previous CS must be

  5. Metal loading in Soda Butte Creek upstream of Yellowstone National Park, Montana and Wyoming; a retrospective analysis of previous research; and quantification of metal loading, August 1999

    Science.gov (United States)

    Boughton, G.K.

    2001-01-01

    Acid drainage from historic mining activities has affected the water quality and aquatic biota of Soda Butte Creek upstream of Yellowstone National Park. Numerous investigations focusing on metals contamination have been conducted in the Soda Butte Creek basin, but interpretations of how metals contamination is currently impacting Soda Butte Creek differ greatly. A retrospective analysis of previous research on metal loading in Soda Butte Creek was completed to provide summaries of studies pertinent to metal loading in Soda Butte Creek and to identify data gaps warranting further investigation. Identification and quantification of the sources of metal loading to Soda Butte Creek was recognized as a significant data gap. The McLaren Mine tailings impoundment and mill site has long been identified as a source of metals but its contribution relative to the total metal load entering Yellowstone National Park was unknown. A tracer-injection and synoptic-sampling study was designed to determine metal loads upstream of Yellowstone National Park.A tracer-injection and synoptic-sampling study was conducted on an 8,511-meter reach of Soda Butte Creek from upstream of the McLaren Mine tailings impoundment and mill site downstream to the Yellowstone National Park boundary in August 1999. Synoptic-sampling sites were selected to divide the creek into discrete segments. A lithium bromide tracer was injected continuously into Soda Butte Creek for 24.5 hours. Downstream dilution of the tracer and current-meter measurements were used to calculate the stream discharge. Stream discharge values, combined with constituent concentrations obtained by synoptic sampling, were used to quantify constituent loading in each segment of Soda Butte Creek.Loads were calculated for dissolved calcium, silica, and sulfate, as well as for dissolved and total-recoverable iron, aluminum, and manganese. Loads were not calculated for cadmium, copper, lead, and zinc because these elements were infrequently

  6. k0-NAA quality assessment by analysis of different certified reference materials using the KAYZERO/SOLCOI software

    International Nuclear Information System (INIS)

    Jacimovic, R.; Bucar, T.; Stegnar, P.; Smodis, B.

    2003-01-01

    A suite of natural matrix reference materials (RMs) were used to assess the quality of analytical results obtained by k 0 -instrumental neutron activation analysis (k 0 -INAA) at the Jozef Stefan Institute (IJS). Five certified reference materials (CRMs) from the Institute for Reference Materials and Measurements (IRMM), two standard reference materials (SRMs) from the National Institute of Standards and Technology (NIST), three RMs from the International Atomic Energy Agency (IAEA) and one RM from IJS were analyzed. Altogether, results for twenty-four elements in inorganic matrices and twenty-nine elements in organic matrices, obtained by k 0 -INAA, were compared to certified values. Results obtained show good agreement with certified or assigned values except for Fe, La, Nd, Sm and U in inorganic matrices, and Ag, Al and Cr in organic matrices. (author)

  7. Determination of tin in biological reference materials by atomic absorption spectrophotometry and neutron activation analysis

    International Nuclear Information System (INIS)

    Chiba, M.; Iyengar, V.; Gills, T.

    1991-01-01

    Because of a lack of reliable analytical techniques for the determination of tin in biological materials, there have been no reference materials certified for this element. However, the authors' experience has shown that it is feasible to use both atomic absorption and nuclear activation techniques at least for selected matrices. Therefore, an investigation was undertaken to determine tin in several biological materials such as non-fat milk powder (NBS-SRM-1549), citrus leaves (NBS-SRM-1572), total diet (NIST-SRM-1548), mixed diet (NBS-RM-8431), and USDIET-I by atomic absorption spectrophotometry (AAS) and neutron activation analysis (NAA). AAS-ashed samples were extracted with MIBK and assayed using a Perkin Elmer model 5000 apparatus. NAA was carried out by irradiating the samples at the NIST reactor in the RT-4 facility and counting with the help of a Ge(Li) detector connected to a multichannel analyzer. The concentration of tin measured by both AAS and NAA agree well for USDIET-I, total diet, citrus leaves and non-fat milk powder (the concentration ranges for tin in these matrices were from 0.0025 to 3.8 micro g/g). However, in the case of mixed diet (RM-8431), the mean values found were 47 ± 5.6 (n = 19) by AAS and 55.5 ± 2.5 (n = 6) by INAA. Since RM-8431 is not certified it is difficult to draw conclusions. For apple and peach leaves, a distillation step was required. The results were apple leaves 0.085 ± 0.015 (n = 10) by AAS and < 0.2 (n = 3) by RNAA; for peach leaves 0.077 ± 0.02 (n = 9) by AAS and < 0.1 (n = 3) by RNAA. All concentrations are expressed in micro g/g dry weight

  8. Automated analysis of retinal images for detection of referable diabetic retinopathy.

    Science.gov (United States)

    Abràmoff, Michael D; Folk, James C; Han, Dennis P; Walker, Jonathan D; Williams, David F; Russell, Stephen R; Massin, Pascale; Cochener, Beatrice; Gain, Philippe; Tang, Li; Lamard, Mathieu; Moga, Daniela C; Quellec, Gwénolé; Niemeijer, Meindert

    2013-03-01

    The diagnostic accuracy of computer detection programs has been reported to be comparable to that of specialists and expert readers, but no computer detection programs have been validated in an independent cohort using an internationally recognized diabetic retinopathy (DR) standard. To determine the sensitivity and specificity of the Iowa Detection Program (IDP) to detect referable diabetic retinopathy (RDR). In primary care DR clinics in France, from January 1, 2005, through December 31, 2010, patients were photographed consecutively, and retinal color images were graded for retinopathy severity according to the International Clinical Diabetic Retinopathy scale and macular edema by 3 masked independent retinal specialists and regraded with adjudication until consensus. The IDP analyzed the same images at a predetermined and fixed set point. We defined RDR as more than mild nonproliferative retinopathy and/or macular edema. A total of 874 people with diabetes at risk for DR. Sensitivity and specificity of the IDP to detect RDR, area under the receiver operating characteristic curve, sensitivity and specificity of the retinal specialists' readings, and mean interobserver difference (κ). The RDR prevalence was 21.7% (95% CI, 19.0%-24.5%). The IDP sensitivity was 96.8% (95% CI, 94.4%-99.3%) and specificity was 59.4% (95% CI, 55.7%-63.0%), corresponding to 6 of 874 false-negative results (none met treatment criteria). The area under the receiver operating characteristic curve was 0.937 (95% CI, 0.916-0.959). Before adjudication and consensus, the sensitivity/specificity of the retinal specialists were 0.80/0.98, 0.71/1.00, and 0.91/0.95, and the mean intergrader κ was 0.822. The IDP has high sensitivity and specificity to detect RDR. Computer analysis of retinal photographs for DR and automated detection of RDR can be implemented safely into the DR screening pipeline, potentially improving access to screening and health care productivity and reducing visual loss

  9. Comparison of extraction procedures for proteome analysis of Streptococcus pneumoniae and a basic reference map.

    Science.gov (United States)

    Encheva, Vesela; Gharbia, Saheer E; Wait, Robin; Begum, Shajna; Shah, Haroun N

    2006-06-01

    Streptococcus pneumoniae is an important human pathogen causing life-threatening invasive diseases such as pneumonia, meningitis and bacteraemia. Despite major advances in our understanding of pneumococcal mechanisms of pathogenicity obtained through genomic studies very little has been achieved on the characterisation of the proteome of this pathogen. The highly complex structure of its cell envelope particularly amongst the various capsular forms enables the cell to resist lysis by conventional mechanical methods. It is therefore highly desirable to develop a cellular lysis and protein solubilisation procedure that minimises protein losses and allows for maximum possible coverage of the proteome of S. pneumoniae. Here we have utilised various combinations of mechanical or enzymatic cell lysis with two protein solubilisation mixtures urea/CHAPS-based mixture or SDS/DTT-based mixture in order to achieve best quality protein profiles using two proteomic technologies surface-enhanced laser desorption ionisation (SELDI) TOF MS and 2-DE. While urea/CHAPS-based mixture combined with freeze/thawing provided enough material for good-quality SELDI TOF MS fingerprints, a combination of mechanical, enzymatic and chemical lysis was needed to be used to successfully extract the desired protein content for 2-DE analysis. The methods chosen were also assessed for reproducibility and tested on various capsular types of S. pneumoniae. As a result, good-quality and reproducible profiles were created using various ProteinChip arrays and more than 800 protein spots were separated on a single 2-D gel of S. pneumoniae. Twenty-five of the most abundant protein spots were identified using LC/MS/MS to create a reference map of S. pneumoniae. The proteins identified included glycolytic enzymes such as glyceraldehyde 3-phosphate dehydrogenase, phosphoglycerate kinase, enolase etc. Several fermentation enzymes were also present including two of the components of the arginine deiminase system

  10. Classification and Regression Tree Analysis of Clinical Patterns that Predict Survival in 127 Chinese Patients with Advanced Non-small Cell Lung Cancer Treated by Gefitinib Who Failed to Previous Chemotherapy

    Directory of Open Access Journals (Sweden)

    Ziping WANG

    2011-09-01

    Full Text Available Background and objective It has been proven that gefitinib produces only 10%-20% tumor regression in heavily pretreated, unselected non-small cell lung cancer (NSCLC patients as the second- and third-line setting. Asian, female, nonsmokers and adenocarcinoma are favorable factors; however, it is difficult to find a patient satisfying all the above clinical characteristics. The aim of this study is to identify novel predicting factors, and to explore the interactions between clinical variables and their impact on the survival of Chinese patients with advanced NSCLC who were heavily treated with gefitinib in the second- or third-line setting. Methods The clinical and follow-up data of 127 advanced NSCLC patients referred to the Cancer Hospital & Institute, Chinese Academy of Medical Sciences from March 2005 to March 2010 were analyzed. Multivariate analysis of progression-free survival (PFS was performed using recursive partitioning, which is referred to as the classification and regression tree (CART analysis. Results The median PFS of 127 eligible consecutive advanced NSCLC patients was 8.0 months (95%CI: 5.8-10.2. CART was performed with an initial split on first-line chemotherapy outcomes and a second split on patients’ age. Three terminal subgroups were formed. The median PFS of the three subsets ranged from 1.0 month (95%CI: 0.8-1.2 for those with progressive disease outcome after the first-line chemotherapy subgroup, 10 months (95%CI: 7.0-13.0 in patients with a partial response or stable disease in first-line chemotherapy and age <70, and 22.0 months for patients obtaining a partial response or stable disease in first-line chemotherapy at age 70-81 (95%CI: 3.8-40.1. Conclusion Partial response, stable disease in first-line chemotherapy and age ≥ 70 are closely correlated with long-term survival treated by gefitinib as a second- or third-line setting in advanced NSCLC. CART can be used to identify previously unappreciated patient

  11. The System For Co-Reference Resolution For Slovenian Texts Analysis and Possibilities of its Use

    Directory of Open Access Journals (Sweden)

    Peter Holozan

    2015-10-01

    Full Text Available Co-reference resolution is an important part of language technologies, but has not yet been developed for Slovenian. There are various types of co-references and the paper focuses on anaphora resolution of personal pronouns. Seven methods, used in combination, were used; the most important one is based on activation. First results are promising, but for more extensive evaluation, Slovenian corpus with marked examples is needed. Co-reference resolution was used in the question answering system Crammer, which can, as a result, answer more questions than before, because it can replace personal pronouns. At the same time, some other improvement were added to Crammer, e.g. answering to individual words and phrases and answering to declarative sentences. Added was also generation of long answers to questions with interrogative particles. Co-reference resolution also improved working of Presis machine translation, especially for determining of gender of pronouns and for disambiguation of attributive subordinate clauses.

  12. Analysis of Self-Reference in Martin Le Maistre's Tractatus Consequentiarum

    Czech Academy of Sciences Publication Activity Database

    Hanke, Miroslav

    2015-01-01

    Roč. 12, č. 1 (2015), s. 57-94 ISSN 1214-8407 Institutional support: RVO:67985955 Keywords : closure principle * Martin Le Maistre * paradox * self-reference * validity Subject RIV: AA - Philosophy ; Religion

  13. Cloning and evaluation of reference genes for quantitative real-time PCR analysis in Amorphophallus.

    Science.gov (United States)

    Wang, Kai; Niu, Yi; Wang, Qijun; Liu, Haili; Jin, Yi; Zhang, Shenglin

    2017-01-01

    Quantitative real-time reverse transcription PCR (RT-qPCR) has been widely used in the detection and quantification of gene expression levels because of its high accuracy, sensitivity, and reproducibility as well as its large dynamic range. However, the reliability and accuracy of RT-qPCR depends on accurate transcript normalization using stably expressed reference genes. Amorphophallus is a perennial plant with a high content of konjac glucomannan (KGM) in its corm. This crop has been used as a food source and as a traditional medicine for thousands of years. Without adequate knowledge of gene expression profiles, there has been no report of validated reference genes in Amorphophallus . In this study, nine genes that are usually used as reference genes in other crops were selected as candidate reference genes. These putative sequences of these genes Amorphophallus were cloned by the use of degenerate primers. The expression stability of each gene was assessed in different tissues and under two abiotic stresses (heat and waterlogging) in A. albus and A. konjac . Three distinct algorithms were used to evaluate the expression stability of the candidate reference genes. The results demonstrated that EF1-a , EIF4A , H3 and UBQ were the best reference genes under heat stress in Amorphophallus . Furthermore, EF1-a , EIF4A , TUB , and RP were the best reference genes in waterlogged conditions. By comparing different tissues from all samples, we determined that EF1-α , EIF4A, and CYP were stable in these sets. In addition, the suitability of these reference genes was confirmed by validating the expression of a gene encoding the small heat shock protein SHSP , which is related to heat stress in Amorphophallus . In sum, EF1-α and EIF4A were the two best reference genes for normalizing mRNA levels in different tissues and under various stress treatments, and we suggest using one of these genes in combination with 1 or 2 reference genes associated with different biological

  14. Cloning and evaluation of reference genes for quantitative real-time PCR analysis in Amorphophallus

    Directory of Open Access Journals (Sweden)

    Kai Wang

    2017-04-01

    Full Text Available Quantitative real-time reverse transcription PCR (RT-qPCR has been widely used in the detection and quantification of gene expression levels because of its high accuracy, sensitivity, and reproducibility as well as its large dynamic range. However, the reliability and accuracy of RT-qPCR depends on accurate transcript normalization using stably expressed reference genes. Amorphophallus is a perennial plant with a high content of konjac glucomannan (KGM in its corm. This crop has been used as a food source and as a traditional medicine for thousands of years. Without adequate knowledge of gene expression profiles, there has been no report of validated reference genes in Amorphophallus. In this study, nine genes that are usually used as reference genes in other crops were selected as candidate reference genes. These putative sequences of these genes Amorphophallus were cloned by the use of degenerate primers. The expression stability of each gene was assessed in different tissues and under two abiotic stresses (heat and waterlogging in A. albus and A. konjac. Three distinct algorithms were used to evaluate the expression stability of the candidate reference genes. The results demonstrated that EF1-a, EIF4A, H3 and UBQ were the best reference genes under heat stress in Amorphophallus. Furthermore, EF1-a, EIF4A, TUB, and RP were the best reference genes in waterlogged conditions. By comparing different tissues from all samples, we determined that EF1-α, EIF4A, and CYP were stable in these sets. In addition, the suitability of these reference genes was confirmed by validating the expression of a gene encoding the small heat shock protein SHSP, which is related to heat stress in Amorphophallus. In sum, EF1-α and EIF4A were the two best reference genes for normalizing mRNA levels in different tissues and under various stress treatments, and we suggest using one of these genes in combination with 1 or 2 reference genes associated with different

  15. Screening Reliable Reference Genes for RT-qPCR Analysis of Gene Expression in Moringa oleifera.

    Science.gov (United States)

    Deng, Li-Ting; Wu, Yu-Ling; Li, Jun-Cheng; OuYang, Kun-Xi; Ding, Mei-Mei; Zhang, Jun-Jie; Li, Shu-Qi; Lin, Meng-Fei; Chen, Han-Bin; Hu, Xin-Sheng; Chen, Xiao-Yang

    2016-01-01

    Moringa oleifera is a promising plant species for oil and forage, but its genetic improvement is limited. Our current breeding program in this species focuses on exploiting the functional genes associated with important agronomical traits. Here, we screened reliable reference genes for accurately quantifying the expression of target genes using the technique of real-time quantitative polymerase chain reaction (RT-qPCR) in M. oleifera. Eighteen candidate reference genes were selected from a transcriptome database, and their expression stabilities were examined in 90 samples collected from the pods in different developmental stages, various tissues, and the roots and leaves under different conditions (low or high temperature, sodium chloride (NaCl)- or polyethyleneglycol (PEG)- simulated water stress). Analyses with geNorm, NormFinder and BestKeeper algorithms revealed that the reliable reference genes differed across sample designs and that ribosomal protein L1 (RPL1) and acyl carrier protein 2 (ACP2) were the most suitable reference genes in all tested samples. The experiment results demonstrated the significance of using the properly validated reference genes and suggested the use of more than one reference gene to achieve reliable expression profiles. In addition, we applied three isotypes of the superoxide dismutase (SOD) gene that are associated with plant adaptation to abiotic stress to confirm the efficacy of the validated reference genes under NaCl and PEG water stresses. Our results provide a valuable reference for future studies on identifying important functional genes from their transcriptional expressions via RT-qPCR technique in M. oleifera.

  16. Rivaroxaban compared with warfarin in patients with atrial fibrillation and previous stroke or transient ischaemic attack: a subgroup analysis of ROCKET AF.

    Science.gov (United States)

    Hankey, Graeme J; Patel, Manesh R; Stevens, Susanna R; Becker, Richard C; Breithardt, Günter; Carolei, Antonio; Diener, Hans-Christoph; Donnan, Geoffrey A; Halperin, Jonathan L; Mahaffey, Kenneth W; Mas, Jean-Louis; Massaro, Ayrton; Norrving, Bo; Nessel, Christopher C; Paolini, John F; Roine, Risto O; Singer, Daniel E; Wong, Lawrence; Califf, Robert M; Fox, Keith A A; Hacke, Werner

    2012-04-01

    In ROCKET AF, rivaroxaban was non-inferior to adjusted-dose warfarin in preventing stroke or systemic embolism among patients with atrial fibrillation (AF). We aimed to investigate whether the efficacy and safety of rivaroxaban compared with warfarin is consistent among the subgroups of patients with and without previous stroke or transient ischaemic attack (TIA). In ROCKET AF, patients with AF who were at increased risk of stroke were randomly assigned (1:1) in a double-blind manner to rivaroxaban 20 mg daily or adjusted dose warfarin (international normalised ratio 2·0-3·0). Patients and investigators were masked to treatment allocation. Between Dec 18, 2006, and June 17, 2009, 14 264 patients from 1178 centres in 45 countries were randomly assigned. The primary endpoint was the composite of stroke or non-CNS systemic embolism. In this substudy we assessed the interaction of the treatment effects of rivaroxaban and warfarin among patients with and without previous stroke or TIA. Efficacy analyses were by intention to treat and safety analyses were done in the on-treatment population. ROCKET AF is registered with ClinicalTrials.gov, number NCT00403767. 7468 (52%) patients had a previous stroke (n=4907) or TIA (n=2561) and 6796 (48%) had no previous stroke or TIA. The number of events per 100 person-years for the primary endpoint in patients treated with rivaroxaban compared with warfarin was consistent among patients with previous stroke or TIA (2·79% rivaroxaban vs 2·96% warfarin; hazard ratio [HR] 0·94, 95% CI 0·77-1·16) and those without (1·44%vs 1·88%; 0·77, 0·58-1·01; interaction p=0·23). The number of major and non-major clinically relevant bleeding events per 100 person-years in patients treated with rivaroxaban compared with warfarin was consistent among patients with previous stroke or TIA (13·31% rivaroxaban vs 13·87% warfarin; HR 0·96, 95% CI 0·87-1·07) and those without (16·69%vs 15·19%; 1·10, 0·99-1·21; interaction p=0·08

  17. Development of a Reference Database for Ion Beam Analysis. Report of a Coordinated Research Project on Reference Database for Ion Beam Analysis

    International Nuclear Information System (INIS)

    2015-12-01

    Ion beam analysis techniques are non-destructive analytical techniques used to identify the composition and provide elemental depth profiles in surface layers of materials. The applications of such techniques are diverse and include environmental control, cultural heritage and conservation and fusion technologies. Their reliability and accuracy depends strongly on our knowledge of the nuclear reaction cross sections, and this publication describes the coordinated effort to measure, compile and evaluate cross section data relevant to these techniques and make these data available to the user community through a comprehensive online database. It includes detailed assessments of experimental cross sections as well as attempts to benchmark these data against appropriate integral measurements

  18. Constructing an indicator of consumption for the analysis of poverty : principles and illustrations with reference to Ecuador

    NARCIS (Netherlands)

    Hentschel, J; Lanjouw, P.

    1996-01-01

    With reference to data from Ecuador this paper examines several of the steps involved in constructing consumption aggregates and highlights some of the principles which should guide the analysis. The paper emphasizes that specific care is warranted where access is characterized by rationing. Simple

  19. Trend analysis and forecast of precipitation, reference evapotranspiration, and rainfall deficit in the Blackland Prairie of eastern Mississippi

    Science.gov (United States)

    Gary Feng; Stacy Cobb; Zaid Abdo; Daniel K. Fisher; Ying Ouyang; Ardeshir Adeli; Johnie N. Jenkins

    2016-01-01

    Trend analysis and estimation of monthly and annual precipitation, reference evapotranspiration ET, and rainfall deficit are essential for water-resources management and cropping-system design. Rainfall, ET, and water-deficit patterns and trends at Macon in eastern Mississippi for a 120-yr period (1894-2014) were analyzed for annual, seasonal, and monthly...

  20. Development of a reference database for ion beam analysis. Summary report of the first research coordination meeting

    International Nuclear Information System (INIS)

    Vickridge, I.; Schwerer, O.

    2006-01-01

    A summary is given of the First Research Coordination Meeting on the Development of a Reference Database for Ion Beam Analysis, including background information, objectives, recommendations for measurements, and a list of tasks assigned to participants. The next research co-ordination meeting will be held in May 2007. (author)

  1. Specifications for trueness and precision of a reference measurement system for serum/plasma 25-hydroxyvitamin D analysis.

    Science.gov (United States)

    Stöckl, Dietmar; Sluss, Patrick M; Thienpont, Linda M

    2009-10-01

    The divergence in analytical quality of serum/plasma 25-hydroxy-vitamin D analysis calls for defining specifications for a reference measurement system. Fundamentally, in a reference measurement system, there should be a relationship between the analytical specifications for higher- (reference) and lower-order (routine) measurements. Therefore, when setting specifications, we started with limits for routine imprecision (CV(rou)) and bias (B(rou)) using 4 models: (1) the misclassifications in diagnosis, (2) biological variation data (reference interval (RI) and monitoring), (3) expert recommendations, and (4) state-of-the-art performance. Then, we used the derived goals to tailor those for reference measurements and certified reference materials (CRMs) for calibration by setting the limits for CV(ref) at 0.5 CV(rou), B(ref) at 0.33 B(rou)(,) max. uncertainty (U(max)) at 0.33 B(ref). The established specifications ranged between CV(rou)model 3) and CV(rou)model 2, monitoring). Model 2 (monitoring) gave the most stringent goals, model 3, the most liberal ones. Accounting for state-of-the-art performance and certification capabilities, we used model 2 (RI) to recommend achievable goals: for routine testing, CV(rou)measurements, CV(ref)

  2. Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous Frankliniella occidentalis.

    Directory of Open Access Journals (Sweden)

    Chunxiao Yang

    Full Text Available Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR is a reliable technique for measuring and evaluating gene expression during variable biological processes. To facilitate gene expression studies, normalization of genes of interest relative to stable reference genes is crucial. The western flower thrips Frankliniella occidentalis (Pergande (Thysanoptera: Thripidae, the main vector of tomato spotted wilt virus (TSWV, is a destructive invasive species. In this study, the expression profiles of 11 candidate reference genes from nonviruliferous and viruliferous F. occidentalis were investigated. Five distinct algorithms, geNorm, NormFinder, BestKeeper, the ΔCt method, and RefFinder, were used to determine the performance of these genes. geNorm, NormFinder, BestKeeper, and RefFinder identified heat shock protein 70 (HSP70, heat shock protein 60 (HSP60, elongation factor 1 α, and ribosomal protein l32 (RPL32 as the most stable reference genes, and the ΔCt method identified HSP60, HSP70, RPL32, and heat shock protein 90 as the most stable reference genes. Additionally, two reference genes were sufficient for reliable normalization in nonviruliferous and viruliferous F. occidentalis. This work provides a foundation for investigating the molecular mechanisms of TSWV and F. occidentalis interactions.

  3. Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous Frankliniella occidentalis.

    Science.gov (United States)

    Yang, Chunxiao; Li, Hui; Pan, Huipeng; Ma, Yabin; Zhang, Deyong; Liu, Yong; Zhang, Zhanhong; Zheng, Changying; Chu, Dong

    2015-01-01

    Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for measuring and evaluating gene expression during variable biological processes. To facilitate gene expression studies, normalization of genes of interest relative to stable reference genes is crucial. The western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), the main vector of tomato spotted wilt virus (TSWV), is a destructive invasive species. In this study, the expression profiles of 11 candidate reference genes from nonviruliferous and viruliferous F. occidentalis were investigated. Five distinct algorithms, geNorm, NormFinder, BestKeeper, the ΔCt method, and RefFinder, were used to determine the performance of these genes. geNorm, NormFinder, BestKeeper, and RefFinder identified heat shock protein 70 (HSP70), heat shock protein 60 (HSP60), elongation factor 1 α, and ribosomal protein l32 (RPL32) as the most stable reference genes, and the ΔCt method identified HSP60, HSP70, RPL32, and heat shock protein 90 as the most stable reference genes. Additionally, two reference genes were sufficient for reliable normalization in nonviruliferous and viruliferous F. occidentalis. This work provides a foundation for investigating the molecular mechanisms of TSWV and F. occidentalis interactions.

  4. Suitable reference genes for accurate gene expression analysis in parsley (Petroselinum crispum for abiotic stresses and hormone stimuli

    Directory of Open Access Journals (Sweden)

    Meng-Yao Li

    2016-09-01

    Full Text Available Parsley is one of the most important vegetable in Apiaceae family and widely used in food industry, medicinal and cosmetic. The recent studies in parsley are mainly focus on chemical composition, further research involving the analysis of the gene functions and expressions will be required. qPCR is a powerful method for detecting very low quantities of target transcript levels and widely used for gene expression studies. To ensure the accuracy of results, a suitable reference gene is necessary for expression normalization. In this study, three software geNorm, NormFinder, and BestKeeper were used to evaluate the expression stabilities of eight candidate reference genes (GAPDH, ACTIN, eIF-4α, SAND, UBC, TIP41, EF-1α, and TUB under various conditions including abiotic stresses (heat, cold, salt, and drought and hormone stimuli treatments (GA, SA, MeJA, and ABA. The results showed that EF-1α and TUB were identified as the most stable genes for abiotic stresses, while EF-1α, GAPDH, and TUB were the top three choices for hormone stimuli treatments. Moreover, EF-1α and TUB were the most stable reference genes across all the tested samples, while UBC was the least stable one. The expression analysis of PcDREB1 and PcDREB2 further verified that the selected stable reference genes were suitable for gene expression normalization. This study provides a guideline for selection the suitable reference genes in gene expression in parsley.

  5. Transcriptional analysis of the mammalian heart with special reference to its endocrine function

    Directory of Open Access Journals (Sweden)

    de Bold Adolfo J

    2009-06-01

    Full Text Available Abstract Background Pharmacological and gene ablation studies have demonstrated the crucial role of the endocrine function of the heart as mediated by the polypeptide hormones ANF and BNP in the maintenance of cardiovascular homeostasis. The importance of these studies lies on the fact that hypertension and chronic congestive heart failure are clinical entities that may be regarded as states of relative deficiency of ANF and BNP. These hormones are produced by the atrial muscle cells (cardiocytes, which display a dual secretory/muscle phenotype. In contrast, ventricular cardiocytes display mainly a muscle phenotype. Comparatively little information is available regarding the genetic background for this important phenotypic difference with particular reference to the endocrine function of the heart. We postulated that comparison of gene expression profiles between atrial and ventricular muscles would help identify gene transcripts that underlie the phenotypic differences associated with the endocrine function of the heart. Results Comparison of gene expression profiles in the rat heart revealed a total of 1415 differentially expressed genes between the atria and ventricles based on a 1.8 fold cut-off. The identification of numerous chamber specific transcripts, such as ANF for the atria and Irx4 for the ventricles among several others, support the soundness of the GeneChip data and demonstrates that the differences in gene expression profiles observed between the atrial and ventricular tissues were not spurious in nature. Pathway analysis revealed unique expression profiles in the atria for G protein signaling that included Gαo1, Gγ2 and Gγ3, AGS1, RGS2, and RGS6 and the related K+ channels GIRK1 and GIRK4. Transcripts involved in vesicle trafficking, hormone secretion as well as mechanosensors (e.g. the potassium channel TREK-1 were identified in relationship to the synthesis, storage and secretion of hormones. Conclusion The data developed

  6. Thyroid Function Tests in the Reference Range and Fracture: Individual Participant Analysis of Prospective Cohorts.

    Science.gov (United States)

    Aubert, Carole E; Floriani, Carmen; Bauer, Douglas C; da Costa, Bruno R; Segna, Daniel; Blum, Manuel R; Collet, Tinh-Hai; Fink, Howard A; Cappola, Anne R; Syrogiannouli, Lamprini; Peeters, Robin P; Åsvold, Bjørn O; den Elzen, Wendy P J; Luben, Robert N; Bremner, Alexandra P; Gogakos, Apostolos; Eastell, Richard; Kearney, Patricia M; Hoff, Mari; Le Blanc, Erin; Ceresini, Graziano; Rivadeneira, Fernando; Uitterlinden, André G; Khaw, Kay-Tee; Langhammer, Arnulf; Stott, David J; Westendorp, Rudi G J; Ferrucci, Luigi; Williams, Graham R; Gussekloo, Jacobijn; Walsh, John P; Aujesky, Drahomir; Rodondi, Nicolas

    2017-08-01

    Hyperthyroidism is associated with increased fracture risk, but it is not clear if lower thyroid-stimulating hormone (TSH) and higher free thyroxine (FT4) in euthyroid individuals are associated with fracture risk. To evaluate the association of TSH and FT4 with incident fractures in euthyroid individuals. Individual participant data analysis. Thirteen prospective cohort studies with baseline examinations between 1981 and 2002. Adults with baseline TSH 0.45 to 4.49 mIU/L. Primary outcome was incident hip fracture. Secondary outcomes were any, nonvertebral, and vertebral fractures. Results were presented as hazard ratios (HRs) with 95% confidence interval (CI) adjusted for age and sex. For clinical relevance, we studied TSH according to five categories: 0.45 to 0.99 mIU/L; 1.00 to 1.49 mIU/L; 1.50 to 2.49 mIU/L; 2.50 to 3.49 mIU/L; and 3.50 to 4.49 mIU/L (reference). FT4 was assessed as study-specific standard deviation increase, because assays varied between cohorts. During 659,059 person-years, 2,565 out of 56,835 participants had hip fracture (4.5%; 12 studies with data on hip fracture). The pooled adjusted HR (95% CI) for hip fracture was 1.25 (1.05 to 1.49) for TSH 0.45 to 0.99 mIU/L, 1.19 (1.01 to 1.41) for TSH 1.00 to 1.49 mIU/L, 1.09 (0.93 to 1.28) for TSH 1.50 to 2.49 mIU/L, and 1.12 (0.94 to 1.33) for TSH 2.50 to 3.49 mIU/L (P for trend = 0.004). Hip fracture was also associated with FT4 [HR (95% CI) 1.22 (1.11 to 1.35) per one standard deviation increase in FT4]. FT4 only was associated with any and nonvertebral fractures. Results remained similar in sensitivity analyses. Among euthyroid adults, lower TSH and higher FT4 are associated with an increased risk of hip fracture. These findings may help refine the definition of optimal ranges of thyroid function tests. Copyright © 2017 Endocrine Society

  7. Descriptions of reference LWR facilities for analysis of nuclear fuel cycles

    Energy Technology Data Exchange (ETDEWEB)

    Schneider, K.J.; Kabele, T.J.

    1979-09-01

    To contribute to the Department of Energy's identification of needs for improved environmental controls in nuclear fuel cycles, a study was made of a light water reactor system. A reference LWR fuel cycle was defined, and each step in this cycle was characterized by facility description and mainline and effluent treatment process performance. The reference fuel cycle uses fresh uranium in light water reactors. Final treatment and ultimate disposition of waste from the fuel cycle steps were not included, and the waste is assumed to be disposed of by approved but currently undefined means. The characterization of the reference fuel cycle system is intended as basic information for further evaluation of alternative effluent control systems.

  8. Descriptions of reference LWR facilities for analysis of nuclear fuel cycles

    International Nuclear Information System (INIS)

    Schneider, K.J.; Kabele, T.J.

    1979-09-01

    To contribute to the Department of Energy's identification of needs for improved environmental controls in nuclear fuel cycles, a study was made of a light water reactor system. A reference LWR fuel cycle was defined, and each step in this cycle was characterized by facility description and mainline and effluent treatment process performance. The reference fuel cycle uses fresh uranium in light water reactors. Final treatment and ultimate disposition of waste from the fuel cycle steps were not included, and the waste is assumed to be disposed of by approved but currently undefined means. The characterization of the reference fuel cycle system is intended as basic information for further evaluation of alternative effluent control systems

  9. Proper use of reference materials for trace element analysis and updates on their availability

    International Nuclear Information System (INIS)

    Snell, J.P.

    2009-01-01

    Full text: As different spectroscopic methods are used to certify reference materials (RMs) for element content, there is close interrelation between development of new methods and availability of new certified reference materials (CRMs). The Institute for Reference Materials and Measurements (IRMM) monitors changing scientific and regulatory interests, and adapts materials to current and predicted needs. This includes the development of CRMs with new matrices, or changing analytes and concentration levels for existing types of CRMs. Recent examples are CRMs to support the EC Directives on air quality and water, for nanoparticle characterization, and progressing international standards for biofuel measurements. User guidance including appropriate CRM selection, and use of uncertainties in comparing results will be highlighted. (author)

  10. Identification of a previously undetected metabolic defect in the Complex II Caenorhabditis elegans mev-1 mutant strain using respiratory control analysis.

    Science.gov (United States)

    Fong, Sheng; Ng, Li Fang; Ng, Li Theng; Moore, Philip K; Halliwell, Barry; Gruber, Jan

    2017-04-01

    Hypometabolism may play an important role in the pathogenesis of ageing and ageing-related diseases. The nematode Caenorhabditis elegans offers the opportunity to study "living mitochondria" in a small (~1 mm) animal replete with a highly stereotypical, yet complex, anatomy and physiology. Basal oxygen consumption rate is often employed as a proxy for energy metabolism in this context. This parameter is traditionally measured using single-chamber Clark electrodes without the addition of metabolic modulators. Recently, multi-well oxygen electrodes, facilitating addition of metabolic modulators and hence study of respiratory control during different mitochondrial respiration states, have been developed. However, only limited official protocols exist for C. elegans, and key limitations of these techniques are therefore unclear. Following modification and testing of some of the existing protocols, we used these methods to explore mitochondrial bioenergetics in live nematodes of an electron transfer chain Complex II mutant strain, mev-1, and identified a previously undetected metabolic defect. We find that mev-1 mutants cannot respond adequately to increased energy demands, suggesting that oxidative phosphorylation is more severely impaired in these animals than has previously been appreciated.

  11. Reliable reference miRNAs for quantitative gene expression analysis of stress responses in Caenorhabditis elegans

    DEFF Research Database (Denmark)

    Kagias, Konstantinos; Podolska, Agnieszka; Pocock, Roger David John

    2014-01-01

    Quantitative real-time PCR (qPCR) has become the "gold standard" for measuring expression levels of individual miRNAs. However, little is known about the validity of reference miRNAs, the improper use of which can result in misleading interpretation of data.......Quantitative real-time PCR (qPCR) has become the "gold standard" for measuring expression levels of individual miRNAs. However, little is known about the validity of reference miRNAs, the improper use of which can result in misleading interpretation of data....

  12. Analysis of reference sources used in drug-related Wikipedia articles*

    Science.gov (United States)

    Koppen, Laura; Phillips, Jennifer; Papageorgiou, Renee

    2015-01-01

    Objective References from drug-related Wikipedia articles and a drug information database were compared. Methods Drugs in Food and Drug Administration (FDA) MedWatch alerts from January–July 2013 were searched in Wikipedia and Lexicomp to compare reference types and to assess the time for drug safety information to be incorporated into Wikipedia articles. Results Wikipedia most commonly cited peer-reviewed journal articles (49.2%) and news articles (12.0%). MedWatch citations were incorporated into Wikipedia on average in 5.9 days. Conclusions Wikipedia cited various sources but may not be a reliable, up-to-date resource for drug safety information. PMID:26213506

  13. Analysis of reference sources used in drug-related Wikipedia articles.

    Science.gov (United States)

    Koppen, Laura; Phillips, Jennifer; Papageorgiou, Renee

    2015-07-01

    References from drug-related Wikipedia articles and a drug information database were compared. Drugs in Food and Drug Administration (FDA) MedWatch alerts from January-July 2013 were searched in Wikipedia and Lexicomp to compare reference types and to assess the time for drug safety information to be incorporated into Wikipedia articles. Wikipedia most commonly cited peer-reviewed journal articles (49.2%) and news articles (12.0%). MedWatch citations were incorporated into Wikipedia on average in 5.9 days. Wikipedia cited various sources but may not be a reliable, up-to-date resource for drug safety information.

  14. Thalidomide for previously untreated elderly patients with multiple myeloma: meta-analysis of 1685 individual patient data from 6 randomized clinical trials

    DEFF Research Database (Denmark)

    Fayers, Peter M; Palumbo, Antonio; Hulin, Cyrille

    2011-01-01

    The role of thalidomide for previously untreated elderly patients with multiple myeloma remains unclear. Six randomized controlled trials, launched in or after 2000, compared melphalan and prednisone alone (MP) and with thalidomide (MPT). The effect on overall survival (OS) varied across trials. We...... interval 0.73-0.94, P = .004), representing increased median OS time of 6.6 months, from 32.7 months (MP) to 39.3 months (MPT). The thalidomide regimen was also associated with superior PFS (hazard ratio = 0.68, 95% confidence interval 0.61-0.76, P ... or better was 59% on MPT and 37% on MP). Although the trials differed in terms of patient baseline characteristics and thalidomide regimens, there was no evidence that treatment affected OS differently according to levels of the prognostic factors. We conclude that thalidomide added to MP improves OS...

  15. Assessment of international reference materials for isotope-ratio analysis (IUPAC Technical Report)

    Science.gov (United States)

    Brand, Willi A.; Coplen, Tyler B.; Vogl, Jochen; Rosner, Martin; Prohaska, Thomas

    2014-01-01

    Since the early 1950s, the number of international measurement standards for anchoring stable isotope delta scales has mushroomed from 3 to more than 30, expanding to more than 25 chemical elements. With the development of new instrumentation, along with new and improved measurement procedures for studying naturally occurring isotopic abundance variations in natural and technical samples, the number of internationally distributed, secondary isotopic reference materials with a specified delta value has blossomed in the last six decades to more than 150 materials. More than half of these isotopic reference materials were produced for isotope-delta measurements of seven elements: H, Li, B, C, N, O, and S. The number of isotopic reference materials for other, heavier elements has grown considerably over the last decade. Nevertheless, even primary international measurement standards for isotope-delta measurements are still needed for some elements, including Mg, Fe, Te, Sb, Mo, and Ge. It is recommended that authors publish the delta values of internationally distributed, secondary isotopic reference materials that were used for anchoring their measurement results to the respective primary stable isotope scale.

  16. Hepatitis C among Egyptian Patients Referred for Bone Marrow Examination: Seroprevalence and Analysis of Hematological Findings

    Directory of Open Access Journals (Sweden)

    Somaia Mohammed Mousa

    2014-01-01

    Full Text Available Hepatitis C is a significant public health problem in Egypt where the highest prevalence (14.7% of hepatitis C virus (HCV exists. HCV prevalence is even higher among clinical populations and groups at risk of exposure to infection. Chronic HCV infection is associated with several hematological complications that may necessitate bone marrow (BM examination. The aim of this study is to estimate HCV prevalence among patients referred for BM examination and to explore hematological and BM findings among HCV positive patients. One hundred adult patients referred for BM examination were included in the study and screened for HCV antibodies. Patients’ clinical, hematological, and BM findings were recorded. The seroprevalence of HCV among patients referred for BM examination was 42%. The most common indication for BM examination among HCV positive patients was peripheral cytopenias (88.1%. The most common cytopenia detected was thrombocytopenia (85.7%. The most common diagnosis among HCV positive patients was hypersplenism (52.4% followed by B-lymphoproliferative disorders (19% and then immune thrombocytopenic purpura (11.9%. In conclusion, HCV prevalence among patients referred for BM examination is higher than that estimated in the general population. Patients with unexplained peripheral cytopenias should be tested for HCV.

  17. Selection of reference genes for expression analysis in the entomophthoralean fungus Pandora neoaphidis

    DEFF Research Database (Denmark)

    Chen, Chun; Xie, Tingna; Ye, Sudan

    2016-01-01

    The selection of suitable reference genes is crucial for accurate quantification of gene expres-sion and can add to our understanding of host–pathogen interactions. To identify suitablereference genes in Pandora neoaphidis, an obligate aphid pathogenic fungus, the expressionof three traditional...

  18. Analysis of Hourly Climatic Variables-Meteorological Test Reference Year for Beijing

    DEFF Research Database (Denmark)

    Qin, Lin

    1997-01-01

    The climatic data which was surveyed from 1981 to 1993 in Beijing was analysed to generate a representative year, a Test Reference Year (TRY). This TRY consists of just one year of hourly data that could yield the same simulation results as the long-term data, thereby minimizing the computation...

  19. Are Mindful Parents More Authoritative and Less Authoritarian? An Analysis of Clinic-Referred Mothers

    Science.gov (United States)

    Williams, Keith L.; Wahler, Robert G.

    2010-01-01

    Forty clinic-referred mothers completed questionnaires describing their children's problems, the mothers' parenting styles, and their everyday mindfulness. Psychometric analyses of the questionnaires showed mother reports to be internally consistent, except for one of the parenting style scales (i.e., permissive style). We dropped the scale and…

  20. Thalidomide for previously untreated elderly patients with multiple myeloma: meta-analysis of 1685 individual patient data from 6 randomized clinical trials

    DEFF Research Database (Denmark)

    Fayers, Peter M; Palumbo, Antonio; Hulin, Cyrille

    2011-01-01

    carried out a meta-analysis of the 1685 individual patients in these trials. The primary endpoint was OS, and progression-free survival (PFS) and 1-year response rates were secondary endpoints. There was a highly significant benefit to OS from adding thalidomide to MP (hazard ratio = 0.83; 95% confidence...

  1. How to Identify Negative Attitudes towards Inclusive Education: Critical Discourse Analysis of Russian Transcripts Using Role and Reference Grammar

    Directory of Open Access Journals (Sweden)

    Mariia Rubtcova

    2016-09-01

    Full Text Available This paper presents the Role and Reference Grammar (RRG analysis that aims to reveal possibilities required for carrying out the interdisciplinary research development within Critical Discourse Analysis (CDA. It takes a closer look at conflicts, considering the example of a conflict situation occurred in reaction to the opening of the inclusive academic programme at one of St. Petersburg’s secondary schools. Role and Reference Grammar application demonstrates that the use of different verb types and macroroles has led to the various interpretations. These findings confirm that RRG could influence the increase of objectivity of the transcript analysis in qualitative social research. RRG provides new information which in combination with other methods can help us to understand the positions of participants involved into conflicts

  2. Identification of housekeeping genes as references for quantitative real-time RT-PCR analysis in Misgurnus anguillicaudatus.

    Science.gov (United States)

    Xia, Xiaohua; Huo, Weiran; Wan, Ruyan; Xia, Xiaopei; Du, Qiyan; Chang, Zhongjie

    2017-12-01

    Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is a well-known method to quantify gene expression by comparing with the reference genes. Generally, housekeeping genes were set as references, as for their stable expression in varying conditions. Here, we try to evaluate few of such genes to identify suitable housekeeping genes as references for qRT-PCR analysis of gene expression in Misgurnus anguillicaudatus. This study evaluated the expression of four commonly used housekeeping genes, i.e. b-actin (ACTB), elongation factor 1 alpha (EF-1a), glyceraldehyde-3-phosphate (GAPDH) and 18S ribosomal RNA (18S rRNA), in gender difference, effects of tissue type, different developmental stages, chemical treatment of embryos/larvae with commonly used vehicles for administration and agents that represent known environmental toxicant. Rank ordering of expression stability was done using geNorm, NormFinder and BestKeeper algorithms. Results suggested that in the qRTPCR test, in all the experimental conditions, EF-1a could be selected as reference gene when analysing a target gene. For the study of different development stages, ACTB could be a candidate as reference gene. For the studies associated with different gender and tissue types, EF-1a would be better targeted as reference gene. Meanwhile, in toxicant treatment, expression of EF-1a seems to be more stable than others and could be considered as reference gene. This study could provide useful guidelines that can be expected to aid M. anguillicaudatus researchers in their initial choice of housekeeping genes for future studies and enable more accurate normalization of gene expression data.

  3. Selection and validation of appropriate reference genes for quantitative real-time PCR analysis in Salvia hispanica.

    Directory of Open Access Journals (Sweden)

    Rahul Gopalam

    Full Text Available Quantitative real-time polymerase chain reaction (qRT-PCR has become the most popular choice for gene expression studies. For accurate expression analysis, it is pertinent to select a stable reference gene to normalize the data. It is now known that the expression of internal reference genes varies considerably during developmental stages and under different experimental conditions. For Salvia hispanica, an economically important oilseed crop, there are no reports of stable reference genes till date. In this study, we chose 13 candidate reference genes viz. Actin11 (ACT, Elongation factor 1-alpha (EF1-α, Eukaryotic translation initiation factor 3E (ETIF3E, alpha tubulin (α-TUB, beta tubulin (β-TUB, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Cyclophilin (CYP, Clathrin adaptor complex (CAC, Serine/threonine-protein phosphatase 2A (PP2A, FtsH protease (FtsH, 18S ribosomal RNA (18S rRNA, S-adenosyl methionine decarboxylase (SAMDC and Rubisco activase (RCA and the expression levels of these genes were assessed in a diverse set of tissue samples representing vegetative stages, reproductive stages and various abiotic stress treatments. Two of the widely used softwares, geNorm and Normfinder were used to evaluate the expression stabilities of these 13 candidate reference genes under different conditions. Results showed that GAPDH and CYP expression remain stable throughout in the different abiotic stress treatments, CAC and PP2A expression were relatively stable under reproductive stages and α-TUB, PP2A and ETIF3E were found to be stably expressed in vegetative stages. Further, the expression levels of Diacylglycerol acyltransferase (DGAT1, a key enzyme in triacylglycerol synthesis was analyzed to confirm the validity of reference genes identified in the study. This is the first systematic study of selection of reference genes in S. hispanica, and will benefit future expression studies in this crop.

  4. Selection of Reference Genes for qRT-PCR Analysis of Gene Expression in Stipa grandis during Environmental Stresses.

    Directory of Open Access Journals (Sweden)

    Dongli Wan

    Full Text Available Stipa grandis P. Smirn. is a dominant plant species in the typical steppe of the Xilingole Plateau of Inner Mongolia. Selection of suitable reference genes for the quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR is important for gene expression analysis and research into the molecular mechanisms underlying the stress responses of S. grandis. In the present study, 15 candidate reference genes (EF1 beta, ACT, GAPDH, SamDC, CUL4, CAP, SNF2, SKIP1, SKIP5, SKIP11, UBC2, UBC15, UBC17, UCH, and HERC2 were evaluated for their stability as potential reference genes for qRT-PCR under different stresses. Four algorithms were used: GeNorm, NormFinder, BestKeeper, and RefFinder. The results showed that the most stable reference genes were different under different stress conditions: EF1beta and UBC15 during drought and salt stresses; ACT and GAPDH under heat stress; SKIP5 and UBC17 under cold stress; UBC15 and HERC2 under high pH stress; UBC2 and UBC15 under wounding stress; EF1beta and UBC17 under jasmonic acid treatment; UBC15 and CUL4 under abscisic acid treatment; and HERC2 and UBC17 under salicylic acid treatment. EF1beta and HERC2 were the most suitable genes for the global analysis of all samples. Furthermore, six target genes, SgPOD, SgPAL, SgLEA, SgLOX, SgHSP90 and SgPR1, were selected to validate the most and least stable reference genes under different treatments. Our results provide guidelines for reference gene selection for more accurate qRT-PCR quantification and will promote studies of gene expression in S. grandis subjected to environmental stress.

  5. Prevalence of high-risk human papillomavirus and cervical intraepithelial neoplasias in a previously unscreened population--a pooled analysis from three studies.

    Science.gov (United States)

    Basu, Partha; Mittal, Srabani; Bhaumik, Suchismita; Mandal, Shyam Sunder; Samaddar, Anusree; Ray, Chinmayi; Siddiqi, Maqsood; Biswas, Jaydip; Sankaranarayanan, Rengaswamy

    2013-04-01

    Population prevalence of human papillomavirus (HPV) and cervical intraepithelial neoplasias (CIN) is an important indicator to judge the disease burden in the community, to monitor the performance of cervical cancer screening program and to assess the impact of HPV vaccination program. India being a country without any cervical cancer screening program has no published data on the population prevalence of CIN and only a few large community-based studies to report the high-risk HPV prevalence. The objective of our study was to study HPV and CIN prevalence in a previously unscreened population. We pooled together the results of three research studies originally designed to assess the performance of visual inspection after acetic acid application and Hybrid Capture 2 (HC 2). Nearly 60% of the screened women had colposcopy irrespective of their screening test results. The diagnosis and grading of cervical neoplasias were based on histology. The age standardized prevalence of HPV by HC 2 test was 6.0%. Age-adjusted prevalence of CIN1 and CIN2 was 2.3% and 0.5%, respectively. The age-adjusted prevalence of CIN3 was 0.4% and that of invasive cancer was 0.2%. The prevalence of high-risk HPV was relatively low in the population we studied, which is reflected in the low prevalence of high-grade CIN. The prevalence of CIN3 remained constant across age groups due to absence of screening. Copyright © 2012 UICC.

  6. A compendium of P- and S-wave velocities from surface-to-borehole logging; summary and reanalysis of previously published data and analysis of unpublished data

    Science.gov (United States)

    Boore, David M.

    2003-01-01

    For over 28 years, the U.S. Geological Survey (USGS) has been acquiring seismic velocity and geologic data at a number of locations in California, many of which were chosen because strong ground motions from earthquakes were recorded at the sites. The method for all measurements involves picking first arrivals of P- and S-waves from a surface source recorded at various depths in a borehole (as opposed to noninvasive methods, such as the SASW method [e.g., Brown et al., 2002]). The results from most of the sites are contained in a series of U.S. Geological Survey Open-File Reports (see References). Until now, none of the results have been available as computer files, and before 1992 the interpretation of the arrival times was in terms of piecemeal interval velocities, with no attempt to derive a layered model that would fit the travel times in an overall sense (the one exception is Porcella, 1984). In this report I reanalyze all of the arrival times in terms of layered models for P- and for S-wave velocities at each site, and I provide the results as computer files. In addition to the measurements reported in the open-file reports, I also include some borehole results from other reports, as well as some results never before published. I include data for 277 boreholes (at the time of this writing; more will be added to the web site as they are obtained), all in California (I have data from boreholes in Washington and Utah, but these will be published separately). I am also in the process of interpreting travel time data obtained using a seismic cone penetrometer at hundreds of sites; these data can be interpreted in the same way of those obtained from surface-to-borehole logging. When available, the data will be added to the web site (see below for information on obtaining data from the World Wide Web (WWW)). In addition to the basic borehole data and results, I provide information concerning strong-motion stations that I judge to be close enough to the boreholes

  7. MinION Analysis and Reference Consortium: Phase 2 data release and analysis of R9.0 chemistry.

    Science.gov (United States)

    Jain, Miten; Tyson, John R; Loose, Matthew; Ip, Camilla L C; Eccles, David A; O'Grady, Justin; Malla, Sunir; Leggett, Richard M; Wallerman, Ola; Jansen, Hans J; Zalunin, Vadim; Birney, Ewan; Brown, Bonnie L; Snutch, Terrance P; Olsen, Hugh E

    2017-01-01

    Long-read sequencing is rapidly evolving and reshaping the suite of opportunities for genomic analysis. For the MinION in particular, as both the platform and chemistry develop, the user community requires reference data to set performance expectations and maximally exploit third-generation sequencing. We performed an analysis of MinION data derived from whole genome sequencing of Escherichia coli K-12 using the R9.0 chemistry, comparing the results with the older R7.3 chemistry. We computed the error-rate estimates for insertions, deletions, and mismatches in MinION reads. Run-time characteristics of the flow cell and run scripts for R9.0 were similar to those observed for R7.3 chemistry, but with an 8-fold increase in bases per second (from 30 bps in R7.3 and SQK-MAP005 library preparation, to 250 bps in R9.0) processed by individual nanopores, and less drop-off in yield over time. The 2-dimensional ("2D") N50 read length was unchanged from the prior chemistry. Using the proportion of alignable reads as a measure of base-call accuracy, 99.9% of "pass" template reads from 1-dimensional ("1D")  experiments were mappable and ~97% from 2D experiments. The median identity of reads was ~89% for 1D and ~94% for 2D experiments. The total error rate (miscall + insertion + deletion ) decreased for 2D "pass" reads from 9.1% in R7.3 to 7.5% in R9.0 and for template "pass" reads from 26.7% in R7.3 to 14.5% in R9.0. These Phase 2 MinION experiments serve as a baseline by providing estimates for read quality, throughput, and mappability. The datasets further enable the development of bioinformatic tools tailored to the new R9.0 chemistry and the design of novel biological applications for this technology. K: thousand, Kb: kilobase (one thousand base pairs), M: million, Mb: megabase (one million base pairs), Gb: gigabase (one billion base pairs).

  8. A novel no-reference objective stereoscopic video quality assessment method based on visual saliency analysis

    Science.gov (United States)

    Yang, Xinyan; Zhao, Wei; Ye, Long; Zhang, Qin

    2017-07-01

    This paper proposes a no-reference objective stereoscopic video quality assessment method with the motivation that making the effect of objective experiments close to that of subjective way. We believe that the image regions with different visual salient degree should not have the same weights when designing an assessment metric. Therefore, we firstly use GBVS algorithm to each frame pairs and separate both the left and right viewing images into the regions with strong, general and week saliency. Besides, local feature information like blockiness, zero-crossing and depth are extracted and combined with a mathematical model to calculate a quality assessment score. Regions with different salient degree are assigned with different weights in the mathematical model. Experiment results demonstrate the superiority of our method compared with the existed state-of-the-art no-reference objective Stereoscopic video quality assessment methods.

  9. Analysis of soil reference materials for vanadium(+5) species by electrothermal atomic absorption spectrometry

    International Nuclear Information System (INIS)

    Mandiwana, Khakhathi L.; Panichev, Nikolay

    2010-01-01

    Solid Certified Reference Materials (CRMs) with known vanadium(+5) content are currently not commercially available. Because of this, vanadium species have been determined in solid CRMs of soil, viz. CRM023-50, CRM024-50, CRM049-50, SQC001 and SQC0012. These CRMs are certified with only total vanadium content. Vanadium(+5) was extracted from soil reference materials with 0.1 M Na 2 CO 3 . The quantification of V(+5) was carried out by electrothermal atomic absorption spectrometry (ET-AAS). The concentration of V(+5) in the analyzed CRMs was found to be ranging between 3.60 and 86.0 μg g -1 . It was also found that SQC001 contains approximately 88% of vanadium as V(+5) species. Statistical evaluation of the results of the two methods by paired t-test was in good agreement at 95% level of confidence.

  10. Geographic Resources Analysis Support System (GRASS) Version 4.0 User’s Reference Manual

    Science.gov (United States)

    1992-06-01

    Default: elevation vh =viewing-height -ight (in meters) of the location from which scenes will be viewed. Default: 30000 sv--;inkvalue Sink factor...initci IV+’U wag -W;IZir \\IIF wink I WiVimkel 1 11 wintri Wiiikel ’Iiipel-F ,IR..SS 4. W()ii~ni ’ I ) U.S. Ann’ (TIRI, 463 m.geo GRASS Reference Manual

  11. Extension of the PSE code NOLOT for transition analysis in rotating reference frames

    OpenAIRE

    Dechamps, Xavier; Hein, Stefan

    2016-01-01

    The present work aims at contributing to a better understanding of the effect of rotation on the stability properties of boundary layers. For this purpose, the Parabolized-Stability-Equations based NOLOT code was extended to rotating reference frames through the inclusion of the centrifugal and Coriolis forces. Stability analyses of three flow configurations were then considered for verification: the rotating Blasius Profile, the flow along a curved wall and the three-dimensional flow due to ...

  12. Structural analysis of eight novel and 112 previously reported missense mutations in the interactive FXI mutation database reveals new insight on FXI deficiency.

    Science.gov (United States)

    Saunders, Rebecca E; Shiltagh, Nuha; Gomez, Keith; Mellars, Gillian; Cooper, Carolyn; Perry, David J; Tuddenham, Edward G; Perkins, Stephen J

    2009-08-01

    Factor XI (FXI) functions in blood coagulation. FXI is composed of four apple (Ap) domains and a serine protease (SP) domain. Deficiency of FXI leads to an injury-related bleeding disorder, which is remarkable for the lack of correlation between bleeding symptoms and FXI coagulant activity (FXI:C). The number of mutations previously reported in our interactive web database (http://www.FactorXI.org) is now significantly increased to 183 through our new patient studies and from literature surveys. Eight novel missense mutations give a total of 120 throughout the FXI gene (F11). The most abundant defects in FXI are revealed to be those from low-protein plasma levels (Type I: CRM-) that originate from protein misfolding, rather than from functional defects (Type II: CRM+). A total of 70 Ap missense mutations were analysed using a consensus Ap domain structure generated from the FXI dimer crystal structure. This showed that all parts of the Ap domain were affected. The 47 SP missense mutations were also distributed throughout the SP domain structure. The periphery of the Ap beta-sheet structure is sensitive to structural perturbation caused by residue changes throughout the Ap domain, yet this beta-sheet is crucial for FXI dimer formation. Residues located at the Ap4:Ap4 interface in the dimer are much less directly involved. We conclude that the abundance of Type I defects in FXI results from the sensitivity of the Ap domain folding to residue changes within this, and discuss how structural knowledge of the mutations improves our understanding of FXI deficiencies.

  13. An analysis of discrepancies between United Kingdom cancer research funding and societal burden and a comparison to previous and United States values.

    Science.gov (United States)

    Carter, Ashley J R; Delarosa, Beverly; Hur, Hannah

    2015-11-02

    Ideally, the allocation of research funding for each specific type of cancer should be proportional to its societal burden. This burden can be estimated with the metric 'years of life lost' (YLL), which combines overall mortality and age at death. Using United Kingdom data from 2010, we compared research funding from the National Cancer Research Institute to this YLL burden metric for 26 types of cancers in order to identify the discrepancies between cancer research funding allocation and societal burden. We also compared these values to United States data from 2010 and United Kingdom data published in 2005. Our study revealed a number of discrepancies between cancer research funding and burden. Some cancers are funded at levels far higher than their relative burden suggests (testicular, leukaemia, Hodgkin's lymphoma, breast, cervical, ovarian, prostate) while other cancers appear under-funded (gallbladder, lung, nasopharyngeal, intestine, stomach, pancreatic, thyroid, oesophageal, liver, kidney, bladder, and brain/central nervous system). United Kingdom funding patterns over the past decade have generally moved to increase funding to previously under-funded cancers with one notable exception showing a converse trend (breast cancer). The broad relationship between United Kingdom and United States funding patterns is similar with a few exceptions (e.g. leukaemia, Hodgkin's lymphoma, prostate, testicular cancer). There are discrepancies between cancer research funding allocation and societal burden in the United Kingdom. These discrepancies are broadly similar in both the United Kingdom and the United States and, while they appear to be improving, this is not consistent across all types of cancer.

  14. Platinum stable isotope analysis of geological standard reference materials by double-spike MC-ICPMS

    DEFF Research Database (Denmark)

    Creech, John Benjamin; Baker, J. A.; Handler, M. R.

    2014-01-01

    (Creech et al., 2013, J. Anal. At. Spectrom. 28, 853-865). The reproducibility in natural samples is evaluated by processing multiple replicates of four standard reference materials, and is conservatively taken to be ca. ±0.088 (2sd). Pt stable isotope data for the full set of reference materials have...... metal standard solution doped with a range of synthetic matrices and results in Pt yields of ≥90% with purity of ≥95%. Using this chemical separation technique, we have separated Pt from 11 international geological standard reference materials comprising of PGE ores, mantle rocks, igneous rocks and one...... sample from the Cretaceous-Paleogene boundary layer. Pt concentrations in these samples range from ca. 5ngg to 4μgg. This analytical method has been shown to have an external reproducibility on δPt (permil difference in the Pt/Pt ratio from the IRMM-010 standard) of ±0.040 (2sd) on Pt solution standards...

  15. Virtual reference service via chat: a comparative analysis of software for chat solutions

    Directory of Open Access Journals (Sweden)

    Natalia Nakano

    2016-12-01

    Full Text Available Introduction: In the early 2000s, ICT allowed university libraries to offer Virtual Reference Service, reference service mediated by software to enable interaction via chat between the librarian and the people in search for information in library collections or services. Objective: The objective of the research that grounds this article was to compare the software used by several institutions to provide Virtual Reference Service. Metodology: The methodology for evaluation and theoretical construction that supported this article consisted of a study on the theoretical contribution produced nationally and internationally on the subject, in addition to an exploratory research conducted on digital environments to assess the design of the chat software, as well as participant observation. Results: The results show and compare the features and characteristics of the analyzed software. Conclusion: The results of this study point to contextual decisions from the institution and the information professionals, according to the needs and profiles of the subjects who interact with the digital environment, outlined by unique parameters in each institution

  16. A Meta-Analysis of Typhoid Diagnostic Accuracy Studies: A Recommendation to Adopt a Standardized Composite Reference.

    Directory of Open Access Journals (Sweden)

    Helen L Storey

    Full Text Available Novel typhoid diagnostics currently under development have the potential to improve clinical care, surveillance, and the disease burden estimates that support vaccine introduction. Blood culture is most often used as the reference method to evaluate the accuracy of new typhoid tests; however, it is recognized to be an imperfect gold standard. If no single gold standard test exists, use of a composite reference standard (CRS can improve estimation of diagnostic accuracy. Numerous studies have used a CRS to evaluate new typhoid diagnostics; however, there is no consensus on an appropriate CRS. In order to evaluate existing tests for use as a reference test or inclusion in a CRS, we performed a systematic review of the typhoid literature to include all index/reference test combinations observed. We described the landscape of comparisons performed, showed results of a meta-analysis on the accuracy of the more common combinations, and evaluated sources of variability based on study quality. This wide-ranging meta-analysis suggests that no single test has sufficiently good performance but some existing diagnostics may be useful as part of a CRS. Additionally, based on findings from the meta-analysis and a constructed numerical example demonstrating the use of CRS, we proposed necessary criteria and potential components of a typhoid CRS to guide future recommendations. Agreement and adoption by all investigators of a standardized CRS is requisite, and would improve comparison of new diagnostics across independent studies, leading to the identification of a better reference test and improved confidence in prevalence estimates.

  17. [Lower uterine segment thickness measurement in pregnant women with previous caesarean section: intra- and interobserver reliability analysis using bi- and tridimensional ultrasonography].

    Science.gov (United States)

    Barra, Daniela de Abreu; Martins, Wellington de Paula; Gallarretta, Francisco Maximilliano; Nastri, Carolina Oliveira; Nicolau, Luis Guilherme; Mauad Filho, Francisco

    2008-03-01

    to compare the intra and interobserver reproducibility of the total thickness measurement of the inferior uterine segment (IUS), through the abdominal route, and of the muscle layer measurement, through the vaginal route, using bi and tridimensional ultrasonography. the IUS thickness measurement of 30 women, between the 36th and 39th weeks of gestation with previous caesarean section, done by two observers, was studied. Abdominal ultrasonography with the patient in both supine and lithotomy position was performed. In the sagittal section, the IUS was identified and four bidimensional images and two tridimensional blocks of the total thickness were collected through the abdominal route, and the same for the muscle layer, through the vaginal route. Tridimensional acquisitions were manipulated in the multiplanar mode. The time was measured with a chronometer. Reproducibility was evaluated by the computation of the absolute difference between measurements, the ratio of differences smaller than 1 mm, the intraclass coefficient (ICC), and the Bland and Altman's concordance limits. the average bidimensional measurement of IUS thickness was 7.4 mm through the abdominal and 2.7 mm through the vaginal route, and the tridimensional measurement was 6.9 mm through the abdominal and 5.1 mm through the vaginal route. Intra- and interobserver reproducibility of vaginal versus abdominal route: smaller absolute difference (0.2-0.4 mm versus 0.8-1.5 mm), greater ratio of differences (85.8-97.8% versus 48.7-72,8%), with p0.05[A1] and similar lower concordance limits (-38 to 3.4 versus -3.6 to 4 mm) for tridimensional ultrasonography and ICC (0.6-0.9 versus 0.7-0.9). from the above, we came to the conclusion that the measurement of the IUS muscle layer, through the vaginal route using tridimensional ultrasonography is more reproducible. Nevertheless, our results do not indicate that this measurement shows any clinical evidence to predict uterine tear, as that was not the aim of this

  18. Preparation and certification of the Polish reference material 'Oriental Tobacco Leaves' (CTA-OTL-1) for inorganic trace analysis

    International Nuclear Information System (INIS)

    Dybczynski, R.; Polkowska-Motrenko, H.; Samczynski, Z.; Szopa, Z.

    1996-01-01

    A new Polish certified reference material 'Oriental Tobacco Leaves' (CTA-OTL-1) for inorganic trace analysis was prepared. Fresh tobacco leaves of variety 'Oriental' were dried, ground and sieved. All precautions were taken to avoid contamination of material with metals. The next step was homogenization. Preliminary homogeneity checking consisted in determining of Ca, Fe and K content by X-ray fluorescence. Final homogeneity testing was performed by neutron activation analysis determining Co, Cr, Fe and Rb. To assure long-term stability, the whole lot of material was sterilized by electron beam radiation. Certification of the candidate reference material was done on the basis of world-wide interlaboratory comparisons exercise in which 61 laboratories, using various analytical methods participated. (author). 30 refs, 12 tabs, 21 figs

  19. Preparation and certification of the Polish reference material `Oriental Tobacco Leaves` (CTA-OTL-1) for inorganic trace analysis

    Energy Technology Data Exchange (ETDEWEB)

    Dybczynski, R.; Polkowska-Motrenko, H.; Samczynski, Z.; Szopa, Z. [Institute of Nuclear Chemistry and Technology, Warsaw (Poland)

    1996-12-31

    A new Polish certified reference material `Oriental Tobacco Leaves` (CTA-OTL-1) for inorganic trace analysis was prepared. Fresh tobacco leaves of variety `Oriental` were dried, ground and sieved. All precautions were taken to avoid contamination of material with metals. The next step was homogenization. Preliminary homogeneity checking consisted in determining of Ca, Fe and K content by X-ray fluorescence. Final homogeneity testing was performed by neutron activation analysis determining Co, Cr, Fe and Rb. To assure long-term stability, the whole lot of material was sterilized by electron beam radiation. Certification of the candidate reference material was done on the basis of world-wide interlaboratory comparisons exercise in which 61 laboratories, using various analytical methods participated. (author). 30 refs, 12 tabs, 21 figs.

  20. Reference genes for gene expression analysis by real-time reverse transcription polymerase chain reaction of renal cell carcinoma.

    Science.gov (United States)

    Bjerregaard, Henriette; Pedersen, Shona; Kristensen, Søren Risom; Marcussen, Niels

    2011-12-01

    Differentiation between malignant renal cell carcinoma and benign oncocytoma is of great importance to choose the optimal treatment. Accurate preoperative diagnosis of renal tumor is therefore crucial; however, existing imaging techniques and histologic examinations are incapable of providing an optimal differentiation profile. Analysis of gene expression of molecular markers is a new possibility but relies on appropriate standardization to compare different samples. The aim of this study was to identify stably expressed reference genes suitable for the normalization of results extracted from gene expression analysis of renal tumors. Expression levels of 8 potential reference genes (ATP5J, HMBS, HPRT1, PPIA, TBP, 18S, GAPDH, and POLR2A) were examined by real-time reverse transcription polymerase chain reaction in tumor and normal tissue from removed kidneys from 13 patients with renal cell carcinoma and 5 patients with oncocytoma. The expression levels of genes were compared by gene stability value M, average gene stability M, pairwise variation V, and coefficient of variation CV. More candidates were not suitable for the purpose, but a combination of HMBS, PPIA, ATP5J, and TBP was found to be the best combination with an average gene stability value M of 0.9 and a CV of 0.4 in the 18 tumors and normal tissues. A combination of 4 genes, HMBS, PPIA, ATP5J, and TBP, is a possible reference in renal tumor gene expression analysis by reverse transcription polymerase chain reaction. A combination of four genes, HMBS, PPIA, ATP5J and TBP, being stably expressed in tissues from RCC is possible reference genes for gene expression analysis.

  1. Analysis of Hourly Climatic Variables-Meteorological Test Reference Year for Beijing

    DEFF Research Database (Denmark)

    Qin, Lin

    1997-01-01

    The climatic data which was surveyed from 1981 to 1993 in Beijing was analysed to generate a representative year, a Test Reference Year (TRY). This TRY consists of just one year of hourly data that could yield the same simulation results as the long-term data, thereby minimizing the computation...... need to assess long-term system performance. It can be used as input data for the simulation of the performance of solar energy and indoor climate systems. The application of this TRY in the simulation of a solar domestic hot water system is also presented....

  2. Analysis of the procedures and criteria for implantation of X-ray beams for ISO 4037 reference at low energies

    International Nuclear Information System (INIS)

    Figueiredo, Marcus Tadeu Tanuri de

    2012-01-01

    According to the radiological protection principles, practices involving ionizing radiation must be planned and carried out under the guarantee that the values of individual doses, the number of people exposed to radiation and the probability of accidental exposures are as low as reasonably achievable. Therefore, radiation dosimetry is required to be done with reliable instruments designed for measuring dosimetric quantities. The reliability of the dosimetry depends on both the calibration of the dosimeter and its proper performance under certain test conditions. The International Standardization Organization (ISO), in order to promote international standardization and metrological coherency, established sets of reference X-ray beams for dosimeter calibration and typing-test; they are expected to be implemented in metrology laboratories in compliance with specific technical requirements. At low energies there are specific requirements that need to be considered in both the implementation of the reference beams and their use in calibration and testing. The present study is an analysis of the procedures and criteria for ISO 4037 reference radiation implementation, at low energies. In the Dosimeter Calibration Laboratory of the Development Center of Nuclear Technology (LCD / CDTN), experiments were performed for determining the X-ray reference radiation parameters. Alternatives to the usual methods for half-value layer determination were studied. Spectra of reference beams were simulated with software and compared with experimental measurements. Different climatic conditions were reproduced in the Laboratory for analysis of its influence on the determination of the beam parameters. Uncertainties in the measurements of studied parameters were evaluated. According to the ISO 4037 criteria, the low energy X-ray beams were considered to be implemented; they allowed the determination of the conversion coefficients from air kerma to the operational radiation protection

  3. Polish reference material: soya bean flour (INCT-SBF-4) for inorganic trace analysis - preparation and certification

    International Nuclear Information System (INIS)

    Polkowska-Motrenko, H.; Dybczynski, R.; Chajduk, E.; Danko, B.; Kulisa, K.; Samczynski, Z.; Sypula, M.; Szopa, Z.

    2006-01-01

    Preparation, examination and certification of the new matrix reference material of biological origin: Soya Bean Flour (INCT-SBF-4) is described. The material was prepared from soya bean grown in India, not genetically modified. After milling, the material was sieved through the 150 mm nylon sieves and stored in a polyethylene (PE) bag. Approximately 50 kg of sieved soya bean flour was collected. Examination by optical microscopy revealed that Martin's diameter of over 90% of particles was below 50 mm. The whole lot of soya bean flour was then homogenized by mixing for 20 hours in a 110 dm 3 PE drum rotated in three directions. Preliminary homogeneity testing by X-ray fluorescence (XRF) method and final checking of homogeneity by neutron activation analysis (NAA) after distribution of the material into containers revealed, that it is sufficiently homogeneous at least for a sample size ≥ 100 mg. In order to assure the long-term stability, all containers with INCT-SBF-4 were sterilized by electron beam radiation. Long-term stability was checked by analyzing concentrations of selected elements in the material stored in the air-conditioned room at 20 o C. Short-term stability was examined by the determination of concentrations of the selected elements in the bottle stored in the CO 2 incubator at 37 o C. The material was certified on the basis of a worldwide interlaboratory comparison, in which 92 laboratories from 19 countries participated providing 1107 laboratory averages (4873 individual determinations) for 58 elements. A method of data evaluation leading to assignment of certified values was the same as that used previously in the Laboratory of the Department of Analytical Chemistry, Institute of Nuclear Chemistry and Technology. The results for a few elements were obtained by definitive methods developed in the Laboratory and used to support the certification process. Analytical uncertainties and stability uncertainties were quantified to arrive at combined

  4. Analysis of References on the Plastic Surgery In-Service Training Exam.

    Science.gov (United States)

    Silvestre, Jason; Zhang, Alicia; Lin, Samuel J

    2016-06-01

    The Plastic Surgery In-Service Training Exam is a knowledge assessment tool widely used during plastic surgery training in the United States. This study analyzed literature supporting correct answer choices to determine highest yield sources, journal publication lag, and journal impact factors. Digital syllabi of 10 consecutive Plastic Surgery In-Service Training Exam administrations (2006 to 2015) were reviewed. The most-referenced articles, journals, and textbooks were determined. Mean journal impact factor and publication lag were calculated and differences were elucidated by section. Two thousand questions and 5386 references were analyzed. From 2006 to 2015, the percentage of journal citations increased, whereas textbook references decreased (p Plastic Surgery (5.6 percent), Journal of Hand Surgery (American volume) (5.1 percent), and Annals of Plastic Surgery (3.8 percent). There was a trend toward less publication lag over the study period (p = 0.05), with a mean publication lag of 9.1 ± 9.0 years for all journal articles. Mean journal impact factor was 2.3 ± 4.3 and lowest for the hand and lower extremity section (1.7 ± 2.8; p Plastic surgery faculty and residents may use these data to facilitate knowledge acquisition during residency.

  5. Measurement standards and the general problem of reference points in chemical analysis

    International Nuclear Information System (INIS)

    Richter, W.; Dube, G.

    2002-01-01

    Besides the measurement standards available in general metrology in the form of the realisations of the units of measurement, measurement standards of chemical composition are needed for the vast field of chemical measurement (measurements of the chemical composition), because it is the main aim of such measurements to quantify non-isolated substances, often in complicated matrices, to which the 'classical' measurement standards and their lower- level derivatives are not directly applicable. At present, material artefacts as well as standard measurement devices serve as chemical measurement standards. These are measurement standards in the full metrological sense only, however, if they are firmly linked to the SI unit in which the composition represented by the standard is expressed. This requirement has the consequence that only a very restricted number of really reliable chemical measurement standards exist at present. Since it is very difficult and time consuming to increase this number substantially and, on the other hand, reliable reference points are increasingly needed for all kinds of chemical measurements, primary methods of measurement and high-level reference measurements will play an increasingly important role for the establishment of worldwide comparability and hence mutual acceptance of chemical measurement results. (author)

  6. Neutron activation analysis of trace elements in a marine sediment reference material

    International Nuclear Information System (INIS)

    Esquivel, D.; Eguren, L.; Montoya, E.

    1993-01-01

    As part of the second intercomparison run of ARCAL-IV, it was analyzed the marine sediment, reference materials PACS-NR CC1 by INAA, in the RP-10 Reactor with a thermal flux of 7,8 x 10 13 n/cm 2 s. The results obtained (± 1σ,n=4) were: Co: 17,2±0,8 ppm; Cr:97,8±0,6 ppm; Fe: 46,0± 0,7 ppm; Na: 31,0±1,09/kg; Sb: 175±5 ppm; in good agreement with the certified values. The results in ppm (±18,n=4), for non certified elements were:Ba: 730±58; Ce: 25±3; Cs: 3,8±0,8; Eu: 1,00±0,03; Hf: 3,30±0,07; Lu:0,25±0,04; Rb: 44,0±0,9; Sc: 14,40±0,04; Sm: 3,4±0,5; Ta: 0,60±0,03; Tb: 0,5±0,05; Th: 3,80±0,02; U: 2,6±0,4; which are in good agreement with other three Latin American and one European INAA Laboratories. It was used the comparative method with AGV-1, GSP-1 and G-2 , USGS reference materials, as standards. (authors). 7 refs., 4 tabs

  7. Metaphoric Reference: An Eye Movement Analysis of Spanish-English and English-Spanish Bilingual Readers

    Directory of Open Access Journals (Sweden)

    Roberto Ramírez Heredia

    2016-03-01

    Full Text Available This study examines the processing of metaphoric reference by bilingual speakers. English dominant, Spanish dominant, and balanced bilinguals read passages in English biasing either a figurative (e.g., describing a weak and soft fighter that always lost and everyone hated or a literal (e.g., describing a donut and bakery shop that made delicious pastries meaning of a critical metaphoric referential description (e.g., creampuff. We recorded the eye movements (first fixation, gaze duration, go-past duration, and total reading time for the critical region, which was a metaphoric referential description in each passage. The results revealed that literal vs. figurative meaning activation was modulated by language dominance, where Spanish dominant bilinguals were more likely to access the literal meaning, and English dominant and balanced bilinguals had access to both the literal and figurative meanings of the metaphoric referential description. Overall, there was a general tendency for the literal interpretation to be more active, as revealed by shorter reading times for the metaphoric reference used literally, in comparison to when it was used figuratively. Results are interpreted in terms of the Graded Salience Hypothesis (Giora, 2002, 2003 and the Literal Salience Model (Cieślicka, 2006, 2015.

  8. Reference Ranges of Reticulocyte Haemoglobin Content in Preterm and Term Infants: A Retrospective Analysis.

    Science.gov (United States)

    Lorenz, Laila; Peter, Andreas; Arand, Jörg; Springer, Fabian; Poets, Christian F; Franz, Axel R

    2017-01-01

    Despite iron supplementation, some preterm infants develop iron deficiency (ID). The optimal iron status parameter for early detection of ID has yet to be determined. To establish reference ranges for reticulocyte haemoglobin content (Ret-He) in preterm and term infants and to identify confounding factors. Retrospective analyses of Ret-He and complete blood count in infants with a clinically indicated blood sample obtained within 24 h after birth. Mean (SD) Ret-He was 30.7 (3.0) pg in very preterm infants with a gestational age (GA) of pH (r = -0.07). There was a slight variation in Ret-He with mode of delivery [normal vaginal delivery: 32.3 (3.2) pg, secondary caesarean section (CS): 31.4 (3.0) pg, instrumental delivery: 31.3 (2.7) pg and elective CS: 31.2 (2.8) pg]. GA at birth has a negligible impact on Ret-He, and the lower limit of the normal reference range in newborns within 24 h after birth can be set to 25 pg. Moreover, Ret-He seems to be a robust parameter which is not influenced by perinatal factors within the first 24 h after birth. © 2016 S. Karger AG, Basel.

  9. Selection of reliable reference genes in Caenorhabditis elegans for analysis of nanotoxicity.

    Directory of Open Access Journals (Sweden)

    Yanqiong Zhang

    Full Text Available Despite rapid development and application of a wide range of manufactured metal oxide nanoparticles (NPs, the understanding of potential risks of using NPs is less completed, especially at the molecular level. The nematode Caenorhabditis elegans (C.elegans has been emerging as an environmental model to study the molecular mechanism of environmental contaminations, using standard genetic tools such as the real-time quantitative PCR (RT-qPCR. The most important factor that may affect the accuracy of RT-qPCR is to choose appropriate genes for normalization. In this study, we selected 13 reference gene candidates (act-1, cdc-42, pmp-3, eif-3.C, actin, act-2, csq-1, Y45F10D.4, tba-1, mdh-1, ama-1, F35G12.2, and rbd-1 to test their expression stability under different doses of nano-copper oxide (CuO 0, 1, 10, and 50 µg/mL using RT-qPCR. Four algorithms, geNorm, NormFinder, BestKeeper, and the comparative ΔCt method, were employed to evaluate these 13 candidates expressions. As a result, tba-1, Y45F10D.4 and pmp-3 were the most reliable, which may be used as reference genes in future study of nanoparticle-induced genetic response using C.elegans.

  10. Selection of reliable reference genes in Caenorhabditis elegans for analysis of nanotoxicity.

    Science.gov (United States)

    Zhang, Yanqiong; Chen, Dongliang; Smith, Michael A; Zhang, Baohong; Pan, Xiaoping

    2012-01-01

    Despite rapid development and application of a wide range of manufactured metal oxide nanoparticles (NPs), the understanding of potential risks of using NPs is less completed, especially at the molecular level. The nematode Caenorhabditis elegans (C.elegans) has been emerging as an environmental model to study the molecular mechanism of environmental contaminations, using standard genetic tools such as the real-time quantitative PCR (RT-qPCR). The most important factor that may affect the accuracy of RT-qPCR is to choose appropriate genes for normalization. In this study, we selected 13 reference gene candidates (act-1, cdc-42, pmp-3, eif-3.C, actin, act-2, csq-1, Y45F10D.4, tba-1, mdh-1, ama-1, F35G12.2, and rbd-1) to test their expression stability under different doses of nano-copper oxide (CuO 0, 1, 10, and 50 µg/mL) using RT-qPCR. Four algorithms, geNorm, NormFinder, BestKeeper, and the comparative ΔCt method, were employed to evaluate these 13 candidates expressions. As a result, tba-1, Y45F10D.4 and pmp-3 were the most reliable, which may be used as reference genes in future study of nanoparticle-induced genetic response using C.elegans.

  11. Determination of trace elements in Brazilian rice grains and in biological reference materials by neutron activation analysis

    International Nuclear Information System (INIS)

    Maihara, V.A.; Vasconcellos, M.B.A.

    1989-01-01

    Instrumental neutron activation analysis was applied to the determination of the elements Na, K, Br, As, Rb, Zn, Co, Fe and Sc in Brazilian rice samples and in biological standards. Hg and Se concentrations were determined by using a simple radiochemical separation. The chemical procedure was carried out by means of distillation of Hg and Se in HBr medium and subsequent precipitation of selenium by sodium methabissulfide and mercury by thioacetamide. The accuracy of the instrumental and radiochemical methods was evaluated by means of analysis of the Reference Materials NBS-Bovine Liver, Bowen's Kale and NBS-Rice Flour. (author) [pt

  12. Analysis of data related to the update of diagnosis reference levels in radiology and nuclear medicine. Assessment 2009-2010

    International Nuclear Information System (INIS)

    Roch, Patrice; Celier, David

    2012-10-01

    This report presents and comments the results of the analysis of 'patient' dosimetric data which radiology and nuclear medicine establishments had to transmit to the IRSN. The first part concerns conventional radiology and the analysis is made from a general point of view, and then by examination type (in the case of adults and of children). A synthesis of results since 2004 is proposed in terms of transmitted data, of data representativeness, of influence of detection technology on the delivered dose, of evolution of diagnosis reference level. With the same approach, the next parts address scanography examinations and nuclear medicine

  13. Transcriptome-Wide Identification of Reference Genes for Expression Analysis of Soybean Responses to Drought Stress along the Day.

    Directory of Open Access Journals (Sweden)

    Juliana Marcolino-Gomes

    Full Text Available The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptome-wide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5 evaluated in different periods of the day.

  14. Selection of reference genes for qRT-PCR analysis of gene expression in sea cucumber Apostichopus japonicus during aestivation

    Science.gov (United States)

    Zhao, Ye; Chen, Muyan; Wang, Tianming; Sun, Lina; Xu, Dongxue; Yang, Hongsheng

    2014-11-01

    Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) is a technique that is widely used for gene expression analysis, and its accuracy depends on the expression stability of the internal reference genes used as normalization factors. However, many applications of qRT-PCR used housekeeping genes as internal controls without validation. In this study, the expression stability of eight candidate reference genes in three tissues (intestine, respiratory tree, and muscle) of the sea cucumber Apostichopus japonicus was assessed during normal growth and aestivation using the geNorm, NormFinder, delta CT, and RefFinder algorithms. The results indicate that the reference genes exhibited significantly different expression patterns among the three tissues during aestivation. In general, the β-tubulin (TUBB) gene was relatively stable in the intestine and respiratory tree tissues. The optimal reference gene combination for intestine was 40S ribosomal protein S18 (RPS18), TUBB, and NADH dehydrogenase (NADH); for respiratory tree, it was β-actin (ACTB), TUBB, and succinate dehydrogenase cytochrome B small subunit (SDHC); and for muscle it was α-tubulin (TUBA) and NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 13 (NDUFA13). These combinations of internal control genes should be considered for use in further studies of gene expression in A. japonicus during aestivation.

  15. Thoracic endovascular aortic repair migration and aortic elongation differentiated using dual reference point analysis.

    Science.gov (United States)

    Alberta, Hillary B; Takayama, Toshio; Panthofer, Annalise; Cambria, Richard P; Farber, Mark A; Jordan, William D; Matsumura, Jon S

    2018-02-01

    We evaluated images of patients undergoing a thoracic endovascular aortic repair procedure using two reference points as a means for differentiating stent graft migration from aortic elongation. Conventional standards define migration of a stent graft as an absolute change in the distance from the distal graft ring to a distal landmark ≥10 mm compared with a baseline measurement. Aortic elongation occurs over time in both healthy individuals and patients with aortic disease. Aortic elongation in patients with stent grafts may result in increased distal thoracic aortic lengths over time. False-positive stent graft migration would be defined when these patients meet the standard definition for migration, even if the stent has not moved in relation to the elongating aorta. This retrospective study evaluated the aortic length of 23 patients treated with the conformable GORE TAG thoracic endoprosthesis (W. L. Gore & Associates, Flagstaff, Ariz) in three clinical trials (dissection, traumatic injury, and aneurysm). Patients who met the standard definition for migration were selected. A standardized protocol was used to measure aortic centerline lengths, including the innominate artery (IA) to the most distal device ring, the IA to the celiac artery (CA), and the distal ring to the CA. Baseline lengths obtained from the first postoperative image were compared with length measurements obtained from the first interval at which they met the standard definition for migration. The conventional standards for migration using a single reference point were compared with the use of dual reference points. Of the 23 patients with endograft changes, 20 were deemed to have aortic elongation rather than true migration. The remaining three patients were deemed to have migration on the basis of the IA to distal ring position compared with the IA to CA length change. The IA to CA interval length change was markedly greater in those with elongation compared with migration (23.8 ± 8.4

  16. Concept, design and capability analysis of the new Deflectometric Flatness Reference at PTB

    International Nuclear Information System (INIS)

    Schulz, M.; Ehret, G.; Stavridis, M.; Elster, C.

    2010-01-01

    At PTB, a new setup for the highly accurate topography measurement of nearly flat optical surfaces is now under construction. The so-called Deflectometric Flatness Reference (DFR) is designed to measure in the direct deflectometric mode by applying an autocollimator and a scanning pentaprism, and in the difference deflectometric mode corresponding to the Extended Shear Angle Difference (ESAD) principle invented by PTB. With the new DFR instrument, horizontally as well as vertically orientated specimens with dimensions of up to 1 m and a mass of up to 120 kg will be measurable. The design of the new instrument is supported by employing a comprehensive simulation environment developed for dimensional measuring machines. The mechanical and optical concept is illustrated together with the current design of the DFR setup. Results from the simulations are presented to derive requirements for tolerated mechanical stage deviations and alignment accuracies.

  17. Age estimation at death using pubic bone analysis of a virtual reference sample.

    Science.gov (United States)

    Savall, Frédéric; Hérin, Fabrice; Peyron, Pierre Antoine; Rougé, Daniel; Baccino, Eric; Saint-Martin, Pauline; Telmon, Norbert

    2018-03-01

    Age at death estimation is a major part of forensic anthropology, but is often poor in the latter decades of life and should benefit from specific population standards. The aim of this study was to test a virtual reference sample with a uniform age distribution in order to improve the accuracy of age estimation for individuals over 40 years of age. We retrospectively built a random virtual reference sample of pubic symphyses from 1100 clinical cases using computed tomography at two French hospitals; this was compared with a test sample (pubic symphyses from 75 corpses undergoing post-mortem computed tomography at a French forensic department) and with the sample originally used in the Suchey-Brooks (SB) system. Inaccuracy and bias were calculated and the proportions of cases in which the real age fell within the estimated age ± 1 standard deviation were calculated. Compared to using the SB sample, using our French sample resulted in a lower inaccuracy for males over 55 years and bias showed that that for males aged 56 to 70 years were less underestimated. Compared to using the SB sample, using our French sample resulted in a lower inaccuracy for females over 70 years and bias showed that that for females aged 56 and older were less underestimated. This study presents a large data set of pubic symphyseal phases from a French virtual sample that allows for improving age estimation accuracy at death, particularly for individuals over 40 years. This kind of material can be useful to improve the age estimation accuracy in a specific region. However, the reliability remains poor and the variability of pubic symphysis morphology related to bone degeneration seems to be an unavoidable limit of the method.

  18. Analysis Relationship Among Descriptor, References and Citation to Contruct the Inherent Structure of Document Collection

    International Nuclear Information System (INIS)

    Hasibuan, Zainal A.; Mustangimah

    2001-01-01

    There are many characteristics can be used to identify a document which cover characteristics of the documents, cited documents, and citing documents This research explored the inherent structure of a document collection as one of main components of information retrieval system. The characteristics examined are: descriptors, references (cited documents), and citations (citing documents). Three independent variables were studied: co-descriptor, bibliographic coupling, and co-citation. A test collection was constructed by searching on a single descriptor i nformation retrieval i n the CD-ROM version of Education Resource Information Clearinghouse (ERIC), covering the period 1981 through 1985. Descriptors were extracted from ERIC; cited and citing documents associated with the test collection were derived from Social Sciences Citation Index (SSCI), covering the period 1981 through 1990. Three hypothesis were tested in this study, that are: (1) the higher the frequency of co-descriptors between documents, the higher the frequencies of their bibliographic coupling and co-citation; (2) the higher the frequency of bibliographic coupling between documents, the higher the frequencies of their co-citation and co-descriptors; and (3) the higher the frequency of co-citation between documents, the higher the frequencies of their co-descriptors and bibliographic coupling. The results showed that all of three hypothesis are supported statistically and there is a significant linear relationship among the observed variables. It is mean that there is a significant relationship among descriptors, references, and citation, so that it can be used to construct the inherent structure of document collection in order to improve information retrieval system performance

  19. Analysis of 2-spar cantilever wings with special reference to torsion and load transference

    Science.gov (United States)

    Kuhn, Paul

    1936-01-01

    This report deals with the analysis of 2-spar cantilever wings in torsion, taking cognizance of the fact that the spars are not independent, but are interconnected by ribs and other structural members. The principles of interaction are briefly explained, showing that the mutual relief action occurring depends on the "pure torsional stiffness" of the wing cross section. Various practical methods of analysis are outlined. The "Friedrichs-Von Karman equations" are shown to require the least amount of labor. Numerical examples by the several methods of analysis are given and the agreement between the calculation and experiment is shown.

  20. Determination of reference values of elements in whole blood of the wistar rats using neutron activation analysis (NAA)

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira, Laura C.; Zamboni, Cibele B., E-mail: laura@ipen.b [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2011-07-01

    Some investigations, especially biochemistry analysis, can be performed using whole blood if the normality limits are established. The present study deals with the determination of reference values for elements of clinical interest, in whole blood of Wistar rats using the Neutron Activation Analysis technique. Usually these small-sized animals are used as guinea-pig on experiments that involves testing new medicines and medical diagnostic studies. In this investigation, the reference values for blood were determined for: Br (0.0011 - 0.0095 gL{sup -1}), Ca (0.0 - 0.66 gL{sup -1}), Cl (2.35 - 4.91 gL{sup -1}), K (1.00 - 3.12 gL{sup -1}), Mg (0.044 - 0.108 gL{sup -1}), Na (1.13 - 3.09 gL{sup -1}) and S (0.53 - 1.81 gL{sup -1}). These data will allow researchers to optimize their studies, both in terms of cost and time by selecting species that fits to the experimental model as a clinical reference as well as performing biochemical analyses in whole blood using small quantities (few {mu}L) compared to the conventional analyses performed in serum (few mL). (author)

  1. Identification of reference genes for expression analysis by real-time quantitative PCR in drought-stressed soybean

    Directory of Open Access Journals (Sweden)

    Renata Stolf-Moreira

    2011-01-01

    Full Text Available The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR, genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: Gmβ-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress, and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity. The raw cycle threshold (Ct data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to evaluate the best reference gene, according to its stability. The GmGAPDH was the least stable gene, with the highest mean values of expression stability (M, and the most stable genes, with the lowest M values, were the Gmβ-actin and GmRNAr18S, when both root and leaves samples were tested. These genes can be used in RT-qPCR as reference gene for expression analysis.

  2. Analysis of cyclic creep and rupture. Part 2: calculation of cyclic reference stresses and ratcheting interaction diagrams

    Energy Technology Data Exchange (ETDEWEB)

    Carter, P. [Stress Engineering Services, Inc., Mason, OH 45040 (United States)]. E-mail: peter.carter@ses-oh.com

    2005-01-01

    Part 1 gives the basis for the use of cyclic reference stresses for high temperature design and assessment. The methodology relies on elastic-plastic calculations for limit loads, ratcheting and shakedown. In this paper we use a commercial non-linear finite element code for these calculations. Two fairly complex and realistic geometries with cyclic loads are analysed, namely a pipe elbow and a traveling thermal shock in a pressurized pipe. The special case of start-up shut-down cycles is also discussed. Creep and rupture predictions may be made from the results. When reference stresses can be economically calculated, their use for high temperature design has the following advantages: - Accuracy. Limit loads, shakedown and ratcheting limits are based on detailed analysis, and do not rely on rules or judgement. - Efficiency. Use of shakedown and ratcheting reference stresses to predict rupture and creep strain, respectively, allowing details of time and temperature to be dealt with as material data, not affecting the analysis. - Factors of safety. For both low and high temperature problems, factors of safety can be determined or applied, based on the real failure boundaries. - Conservatism. The rupture and strain calculations reflect the limit of rapid cycle behaviour. Cycles with relaxation will be associated with longer lives.

  3. Determination of reference values of elements in whole blood of the wistar rats using neutron activation analysis (NAA)

    International Nuclear Information System (INIS)

    Oliveira, Laura C.; Zamboni, Cibele B.

    2011-01-01

    Some investigations, especially biochemistry analysis, can be performed using whole blood if the normality limits are established. The present study deals with the determination of reference values for elements of clinical interest, in whole blood of Wistar rats using the Neutron Activation Analysis technique. Usually these small-sized animals are used as guinea-pig on experiments that involves testing new medicines and medical diagnostic studies. In this investigation, the reference values for blood were determined for: Br (0.0011 - 0.0095 gL -1 ), Ca (0.0 - 0.66 gL -1 ), Cl (2.35 - 4.91 gL -1 ), K (1.00 - 3.12 gL -1 ), Mg (0.044 - 0.108 gL -1 ), Na (1.13 - 3.09 gL -1 ) and S (0.53 - 1.81 gL -1 ). These data will allow researchers to optimize their studies, both in terms of cost and time by selecting species that fits to the experimental model as a clinical reference as well as performing biochemical analyses in whole blood using small quantities (few μL) compared to the conventional analyses performed in serum (few mL). (author)

  4. Characterization of reference genes for qPCR analysis in various tissues of the Fujian oyster Crassostrea angulata

    Science.gov (United States)

    Pu, Fei; Yang, Bingye; Ke, Caihuan

    2015-07-01

    Accurate quantification of transcripts using quantitative real-time polymerase chain reaction (qPCR) depends on the identification of reliable reference genes for normalization. This study aimed to identify and validate seven reference genes, including actin-2 ( ACT-2), elongation factor 1 alpha ( EF-1α), elongation factor 1 beta ( EF-1β), glyceraldehyde-3-phosphate dehydrogenase ( GAPDH), ubiquitin ( UBQ), β-tubulin ( β-TUB), and 18S ribosomal RNA, from Crassostrea angulata, a valuable marine bivalve cultured worldwide. Transcript levels of the candidate reference genes were examined using qPCR analysis and showed differential expression patterns in the mantle, gill, adductor muscle, labial palp, visceral mass, hemolymph and gonad tissues. Quantitative data were analyzed using the geNorm software to assess the expression stability of the candidate reference genes, revealing that β-TUB and UBQ were the most stable genes. The commonly used GAPDH and 18S rRNA showed low stability, making them unsuitable candidates in this system. The expression pattern of the G protein β-subunit gene ( Gβ) across tissue types was also examined and normalized to the expression of each or both of UBQ and β-TUB as internal controls. This revealed consistent trends with all three normalization approaches, thus validating the reliability of UBQ and β-TUB as optimal internal controls. The study provides the first validated reference genes for accurate data normalization in transcript profiling in Crassostrea angulata, which will be indispensable for further functional genomics studies in this economically valuable marine bivalve.

  5. Compilation of references, data sources and analysis methods for LMFBR primary piping system components

    International Nuclear Information System (INIS)

    Reich, M.; Esztergar, E.P.; Ellison, E.G.; Erdogan, F.; Gray, T.G.F.; Wells, C.W.

    1977-03-01

    A survey and review program for application of fracture mechanics methods in elevated temperature design and safety analysis has been initiated in December of 1976. This is the first of a series of reports, the aim of which is to provide a critical review of the theories of fracture and the application of fracture mechanics methods to life prediction, reliability and safety analysis of piping components in nuclear plants undergoing sub-creep and elevated temperature service conditions

  6. Systems analysis programs for hands-on integrated reliability evaluations (SAPHIRE) version 5.0, technical reference manual

    International Nuclear Information System (INIS)

    Russell, K.D.; Atwood, C.L.; Galyean, W.J.; Sattison, M.B.; Rasmuson, D.M.

    1994-07-01

    The Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) refers to a set of several microcomputer programs that were developed to create and analyze probabilistic risk assessments (PRAs), primarily for nuclear power plants. This volume provides information on the principles used in the construction and operation of Version 5.0 of the Integrated Reliability and Risk Analysis System (IRRAS) and the System Analysis and Risk Assessment (SARA) system. It summarizes the fundamental mathematical concepts of sets and logic, fault trees, and probability. This volume then describes the algorithms that these programs use to construct a fault tree and to obtain the minimal cut sets. It gives the formulas used to obtain the probability of the top event from the minimal cut sets, and the formulas for probabilities that are appropriate under various assumptions concerning repairability and mission time. It defines the measures of basic event importance that these programs can calculate. This volume gives an overview of uncertainty analysis using simple Monte Carlo sampling or Latin Hypercube sampling, and states the algorithms used by these programs to generate random basic event probabilities from various distributions. Further references are given, and a detailed example of the reduction and quantification of a simple fault tree is provided in an appendix

  7. Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome

    Directory of Open Access Journals (Sweden)

    Dougan Gordon

    2009-12-01

    Full Text Available Abstract Background Host defense peptides are a critical component of the innate immune system. Human alpha- and beta-defensin genes are subject to copy number variation (CNV and historically the organization of mouse alpha-defensin genes has been poorly defined. Here we present the first full manual genomic annotation of the mouse defensin region on Chromosome 8 of the reference strain C57BL/6J, and the analysis of the orthologous regions of the human and rat genomes. Problems were identified with the reference assemblies of all three genomes. Defensins have been studied for over two decades and their naming has become a critical issue due to incorrect identification of defensin genes derived from different mouse strains and the duplicated nature of this region. Results The defensin gene cluster region on mouse Chromosome 8 A2 contains 98 gene loci: 53 are likely active defensin genes and 22 defensin pseudogenes. Several TATA box motifs were found for human and mouse defensin genes that likely impact gene expression. Three novel defensin genes belonging to the Cryptdin Related Sequences (CRS family were identified. All additional mouse defensin loci on Chromosomes 1, 2 and 14 were annotated and unusual splice variants identified. Comparison of the mouse alpha-defensins in the three main mouse reference gene sets Ensembl, Mouse Genome Informatics (MGI, and NCBI RefSeq reveals significant inconsistencies in annotation and nomenclature. We are collaborating with the Mouse Genome Nomenclature Committee (MGNC to establish a standardized naming scheme for alpha-defensins. Conclusions Prior to this analysis, there was no reliable reference gene set available for the mouse strain C57BL/6J defensin genes, demonstrating that manual intervention is still critical for the annotation of complex gene families and heavily duplicated regions. Accurate gene annotation is facilitated by the annotation of pseudogenes and regulatory elements. Manually curated gene

  8. Analysis and prediction of reference evapotranspiration with climate change in Xiangjiang River Basin, China

    Directory of Open Access Journals (Sweden)

    Xin-e Tao

    2015-10-01

    Full Text Available Reference evapotranspiration (ET0 is often used to estimate actual evapotranspiration in water balance studies. In this study, the present and future spatial distributions and temporal trends of ET0 in the Xiangjiang River Basin (XJRB in China were analyzed. ET0 during the period from 1961 to 2010 was calculated with historical meteorological data using the FAO Penman-Monteith (FAO P-M method, while ET0 during the period from 2011 to 2100 was downscaled from the Coupled Model Intercomparison Project Phase 5 (CMIP5 outputs under two emission scenarios, representative concentration pathway 4.5 and representative concentration pathway 8.5 (RCP45 and RCP85, using the statistical downscaling model (SDSM. The spatial distribution and temporal trend of ET0 were interpreted with the inverse distance weighted (IDW method and Mann-Kendall test method, respectively. Results show that: (1 the mean annual ET0 of the XJRB is 1 006.3 mm during the period from 1961 to 2010, and the lowest and highest values are found in the northeast and northwest parts due to the high latitude and spatial distribution of climatic factors, respectively; (2 the SDSM performs well in simulating the present ET0 and can be used to predict the future ET0 in the XJRB; and (3 CMIP5 predicts upward trends in annual ET0 under the RCP45 and RCP85 scenarios during the period from 2011 to 2100. Compared with the reference period (1961–1990, ET0 increases by 9.8%, 12.6%, and 15.6% under the RCP45 scenario and 10.2%, 19.1%, and 27.3% under the RCP85 scenario during the periods from 2011 to 2040, from 2041 to 2070, and from 2071 to 2100, respectively. The predicted increasing ET0 under the RCP85 scenario is greater than that under the RCP45 scenario during the period from 2011 to 2100.

  9. Reference values comparisons between DMD and C57B whole blood mice using neutron activation analysis

    International Nuclear Information System (INIS)

    Metairon, Sabrina; Zamboni, Cibele Bugno; Suzuki, Miriam F.; Sant'Anna, Oswaldo A.; Carvalho, Maria Denise F.

    2010-01-01

    Full text: In health area animal models are used to perform clinical investigations, for example, to test new medicines for medical diagnostic and treatment investigations before to be applied in human being. Currently, the conventional bio chemistries analyses are performed using serum, but when small size animal model is involved the biological material can be scarce restricting its collection. In the last years the NAA has been successfully applied at LEER at IPEN for investigation of several elements in blood of small sized animals, resulting in an efficiency procedure for clinical practice. The advantage in using whole blood is relate to the fact that this nuclear procedure needs small quantity of biological material (10 to 100 ?L of whole blood ) when compared with the conventional analyses (0.5 to 1.0 ml of serum). But, to use whole blood to perform these biochemistry investigations it is essential to establish the reference value in blood for the species or animal models. In this study we intend to evaluate a normal range of Br, Ca, Cl, K, Mg and Na concentrations in whole blood using NAA in samples of DMDmdx and C57B/6J mice model used for muscular dystrophy investigations. Thirty whole blood samples were analyzed in the IEA R1- nuclear reactor at IPEN (Sao Paulo, Brazil). These data contribute for applications in veterinary medicine related to biochemistry analyses using whole blood. (author)

  10. Dose area product measurement for diagnostic reference levels and analysis of patient dose in dental radiography

    International Nuclear Information System (INIS)

    Han, S.; Lee, B.; Shin, G.; Choi, J.; Kim, J.; Park, C.; Park, H.; Lee, K.; Kim, Y.

    2008-01-01

    In this study, diagnostic reference levels (DRLs) were suggested and patient doses were analysed through the dose-area product value in dental radiography. In intraoral radiography, at three sites, i.e. molar, premolar and incisor on the maxilla and acquired third quartile values: 55.5, 46 and 36.5 mGy cm 2 , respectively, were measured. In panoramic, cephalo-metric and cone beam computed tomography, the values were 120.3, 146 and 3203 mGy cm 2 (16 x 18 cm), respectively. It has been shown that, in intraoral radiography, the patient dose changes proportionally to the value of mA s, but the change in extra-oral radiography in response to mA s could not be confirmed. The authors could confirm, however, the difference in dose according to the manufacturer in all dental radiography examinations, except for panoramic radiography. Depending on the size of hospital, there were some differences in patient dose in intraoral radiography, but no difference in patient dose in extra-oral radiography. (authors)

  11. MeerKAT time and frequency reference optical network: Preliminary design analysis

    Directory of Open Access Journals (Sweden)

    Enoch K. Rotich Kipnoo

    2017-05-01

    Full Text Available The MeerKAT telescope is a precursor to the Square Kilometre Array, which will rely on optical fibres to link the telescope receivers to a central processor point. The main aspects to consider for the fibre transport are astronomical data transmission as well as timing, monitoring and control. The astronomical data streams from individual dishes to a central building, while the clock signal is distributed from a central point to remote dishes in the telescope array. The MeerKAT telescope, for instance, demands highly accurate and stable clock distribution over up to 12 km of optical fibre to remote dishes. The clock distribution is required for digitisation of astronomical signals. Phase stability is thus critical both for short-term and long-term requirements. In this work, we focused on the short-term stability. Phase noise measurements were performed on optical transmitters used to distribute the clock signals so as to ascertain their contribution to the overall clock jitter of the system. A maximum jitter requirement of 130 fs for a 1.712-GHz clock signal for MeerKAT time and reference is achieved using a distributed feedback laser. We found that with optimised modulation depth, additional passive optical components in the link do not significantly degrade the phase noise response. A distributed feedback laser was proven to be a suitable optical source that will meet the performance and link budget requirements for the MeerKAT telescope.

  12. Determination of the input parameters for inelastic background analysis combined with HAXPES using a reference sample

    DEFF Research Database (Denmark)

    Zborowski, C.; Renault, O; Torres, A

    2018-01-01

    The recent progress in HAXPES combined with Inelastic Background Analysis makes this method a powerful, non-destructive solution to get quantitative information on deeply buried layers and interfaces at depths up to 70. nm. However, we recently highlighted the need for carefully choosing the scat......The recent progress in HAXPES combined with Inelastic Background Analysis makes this method a powerful, non-destructive solution to get quantitative information on deeply buried layers and interfaces at depths up to 70. nm. However, we recently highlighted the need for carefully choosing...

  13. Determination of silver in biological reference materials by neutron activation analysis

    Czech Academy of Sciences Publication Activity Database

    Mizera, Jiří; Řanda, Zdeněk; Kučera, Jan

    2008-01-01

    Roč. 278, č. 3 (2008), s. 599-602 ISSN 0236-5731. [ Tokyo Metropolitan University, Hachioji, 16.09.2007-21.09.2007] Institutional research plan: CEZ:AV0Z10480505 Keywords : Silver * neutron activation analysis * INAA Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders Impact factor: 0.659, year: 2008

  14. Quantitative analysis of Si1-xGex alloy films by SIMS and XPS depth profiling using a reference material

    Science.gov (United States)

    Oh, Won Jin; Jang, Jong Shik; Lee, Youn Seoung; Kim, Ansoon; Kim, Kyung Joong

    2018-02-01

    Quantitative analysis methods of multi-element alloy films were compared. The atomic fractions of Si1-xGex alloy films were measured by depth profiling analysis with secondary ion mass spectrometry (SIMS) and X-ray Photoelectron Spectroscopy (XPS). Intensity-to-composition conversion factor (ICF) was used as a mean to convert the intensities to compositions instead of the relative sensitivity factors. The ICFs were determined from a reference Si1-xGex alloy film by the conventional method, average intensity (AI) method and total number counting (TNC) method. In the case of SIMS, although the atomic fractions measured by oxygen ion beams were not quantitative due to severe matrix effect, the results by cesium ion beam were very quantitative. The quantitative analysis results by SIMS using MCs2+ ions are comparable to the results by XPS. In the case of XPS, the measurement uncertainty was highly improved by the AI method and TNC method.

  15. Effect of B vitamins and lowering homocysteine on cognitive impairment in patients with previous stroke or transient ischemic attack: a prespecified secondary analysis of a randomized, placebo-controlled trial and meta-analysis.

    Science.gov (United States)

    Hankey, Graeme J; Ford, Andrew H; Yi, Qilong; Eikelboom, John W; Lees, Kennedy R; Chen, Christopher; Xavier, Denis; Navarro, Jose C; Ranawaka, Udaya K; Uddin, Wasim; Ricci, Stefano; Gommans, John; Schmidt, Reinhold; Almeida, Osvaldo P; van Bockxmeer, Frank M

    2013-08-01

    High plasma total homocysteine (tHcy) has been associated with cognitive impairment but lowering tHcy with B-vitamins has produced equivocal results. We aimed to determine whether B-vitamin supplementation would reduce tHcy and the incidence of new cognitive impairment among individuals with stroke or transient ischemic attack≥6 months previously. A total of 8164 patients with stroke or transient ischemic attack were randomly allocated to double-blind treatment with one tablet daily of B-vitamins (folic acid, 2 mg; vitamin B6, 25 mg; vitamin B12, 500 μg) or placebo and followed up for 3.4 years (median) in the VITAmins TO Prevent Stroke (VITATOPS) trial. For this prespecified secondary analysis of VITATOPS, the primary outcome was a new diagnosis of cognitive impairment, defined as a Mini-Mental State Examination (MMSE) score6 months after the qualifying stroke; 2608 participants were cognitively unimpaired (MMSE≥24), of whom 2214 participants (1110 B-vitamins versus 1104 placebo) had follow-up MMSEs during 2.8 years (median). At final follow-up, allocation to B-vitamins, compared with placebo, was associated with a reduction in mean tHcy (10.2 μmol/L versus 14.2 μmol/L; Pvitamin B6, and vitamin B12 to a self-selected clinical trial cohort of cognitively unimpaired patients with previous stroke or transient ischemic attack lowered mean tHcy but had no effect on the incidence of cognitive impairment or cognitive decline, as measured by the MMSE, during a median of 2.8 years. URL: http://www.controlled-trials.com. Unique identifier: ISRCTN74743444; URL: http://www.clinicaltrials.gov. Unique identifier: NCT00097669.

  16. Safety analysis of the IAEA reference research reactor during loss of flow accident using the code MERSAT

    International Nuclear Information System (INIS)

    Hainoun, A.; Ghazi, N.; Abdul-Moaiz, B. Mansour

    2010-01-01

    Using the thermal hydraulic code MERSAT detailed model including primary and secondary loop was developed for the IAEA's reference research reactor MTR 10 MW. The developed model enables the simulation of expected neutronic and thermal hydraulic phenomena during normal operation, reactivity and loss of flow accidents. Two different loss of flow accident (LOFA) have been simulated using slow and fast decrease time of core mass flow. In both cases the expected flow reversal from downward forced to upward natural circulation has been successfully simulated. The results indicate that in both accidents the limit of onset of subcooled boiling was not arrived and consequently no exceed of design limits in term of thermal hydraulic instability or DNB is observed. Finally, the simulation results show good agreement with previous international benchmark analyses accomplished with other qualified channel and thermal hydraulic system codes.

  17. Advancements in Particle Analysis Procedures and their Application to the Characterization of Reference Materials for Safeguards

    International Nuclear Information System (INIS)

    Admon, U.; Chinea-Cano, E.; Dzigal, N.; Vogt, K.S.; Halevy, I.; Boblil, E.; Elkayam, T.; Weiss, A.

    2015-01-01

    Two approaches may be employed in the preparation of Reference Materials (RMs) for use in micro analytical techniques: placement of characterized micro artefacts in bulk materials and characterization of certain classes of individual particles in existing materials. In November 2013, a collaborative project was launched with the aim of adding information about such individual particles in existing RMs. The motivation behind this project was to investigate and characterize micro-artefacts present in certain commercially available RM, making them available and fit for use in safeguards and several other nuclear applications. The implementation and development of new techniques for particle characterization in bulk materials are also part of this project. The strategy for that approach includes the following steps: 1. Sample preparation: Dispersion of particles on stubs and planchets by an in-house shock-wave device. 2. Particle-of-Interest identification and characterization: (a) Fission Track (FT) route: Mosaic imaging of detectors containing FT stars; Applying automatic pattern recognition and localization of FT stars in detectors; Using Laser Micro-Dissection (LMD) for retrieval of individual particles; Preparation of sampled particles for SEM observation and other analytical techniques. (b) Alpha Track (αT) route: Direct particle identification and localization using position sensitive detectors (instrumental auto-radiography). (c) The advanced SEM route: Integration of analytical SEM techniques for characterization of individual particles of interest: EDS, mass spectrometry, FIB, micro-Raman. Preliminary results of the ongoing efforts will be reported. Utilization of these hyphenated techniques and instruments represents an innovative approach to particle characterization for Safeguards applications. (author)

  18. Analysis of micro matter reference materials of lichen and algae by SRXRF and PIXE

    International Nuclear Information System (INIS)

    Yin Sha Xin; Wang Jian Liu; Zhang Peiqun

    2002-01-01

    In the present work two nuclear micro analysis techniques, synchrotron radiation (SRXRF) and macro proton induced X ray emission (macro PIXE), were used in the homogeneity test of Algae IAEA-413 and Lichen IAEA-338, and the certification of their elemental contents too. Finally, the Ingamell's sampling constant Ks and the relative homogeneity factor HE of some elements in these two RMs were estimated on the base of our macro PIXE results. (author)

  19. Light Water Reactor Sustainability Program A Reference Plan for Control Room Modernization: Planning and Analysis Phase

    Energy Technology Data Exchange (ETDEWEB)

    Jacques Hugo; Ronald Boring; Lew Hanes; Kenneth Thomas

    2013-09-01

    The U.S. Department of Energy’s Light Water Reactor Sustainability (LWRS) program is collaborating with a U.S. nuclear utility to bring about a systematic fleet-wide control room modernization. To facilitate this upgrade, a new distributed control system (DCS) is being introduced into the control rooms of these plants. The DCS will upgrade the legacy plant process computer and emergency response facility information system. In addition, the DCS will replace an existing analog turbine control system with a display-based system. With technology upgrades comes the opportunity to improve the overall human-system interaction between the operators and the control room. To optimize operator performance, the LWRS Control Room Modernization research team followed a human-centered approach published by the U.S. Nuclear Regulatory Commission. NUREG-0711, Rev. 3, Human Factors Engineering Program Review Model (O’Hara et al., 2012), prescribes four phases for human factors engineering. This report provides examples of the first phase, Planning and Analysis. The three elements of Planning and Analysis in NUREG-0711 that are most crucial to initiating control room upgrades are: • Operating Experience Review: Identifies opportunities for improvement in the existing system and provides lessons learned from implemented systems. • Function Analysis and Allocation: Identifies which functions at the plant may be optimally handled by the DCS vs. the operators. • Task Analysis: Identifies how tasks might be optimized for the operators. Each of these elements is covered in a separate chapter. Examples are drawn from workshops with reactor operators that were conducted at the LWRS Human System Simulation Laboratory HSSL and at the respective plants. The findings in this report represent generalized accounts of more detailed proprietary reports produced for the utility for each plant. The goal of this LWRS report is to disseminate the technique and provide examples sufficient to

  20. Modeling, analysis, and design of stationary reference frame droop controlled parallel three-phase voltage source inverters

    DEFF Research Database (Denmark)

    Vasquez, Juan Carlos; Guerrero, Josep M.; Savaghebi, Mehdi

    2011-01-01

    Power electronics based microgrids consist of a number of voltage source inverters (VSIs) operating in parallel. In this paper, the modeling, control design, and stability analysis of three-phase VSIs are derived. The proposed voltage and current inner control loops and the mathematical models...... of the VSIs were based on the stationary reference frame. A hierarchical control for the paralleled VSI system was developed based on three levels. The primary control includes the droop method and the virtual impedance loops, in order to share active and reactive power. The secondary control restores...

  1. Systems Analysis Programs for Hands-On Integrated Reliability Evaluations (SAPHIRE) Technical Reference

    Energy Technology Data Exchange (ETDEWEB)

    C. L. Smith; W. J. Galyean; S. T. Beck

    2008-08-01

    The Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) is a software application developed for performing a complete probabilistic risk assessment (PRA) using a personal computer (PC) running the Microsoft Windows? operating system. Herein information is provided on the principles used in the construction and operation of Version 6.0 and 7.0 of the SAPHIRE system. This report summarizes the fundamental mathematical concepts of sets and logic, fault trees, and probability. This volume then describes the algorithms used to construct a fault tree and to obtain the minimal cut sets. It gives the formulas used to obtain the probability of the top event from the minimal cut sets, and the formulas for probabilities that apply for various assumptions concerning reparability and mission time. It defines the measures of basic event importance that SAPHIRE can calculate. This volume gives an overview of uncertainty analysis using simple Monte Carlo sampling or Latin Hypercube sampling, and states the algorithms used by this program to generate random basic event probabilities from various distributions. Also covered are enhance capabilities such as seismic analysis, cut set "recovery," end state manipulation, and use of "compound events."

  2. Systems Analysis Programs for Hands-On Integrated Reliability Evaluations (SAPHIRE) Technical Reference Manual

    Energy Technology Data Exchange (ETDEWEB)

    C. L. Smith; W. J. Galyean; S. T. Beck

    2006-07-01

    The Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) is a software application developed for performing a complete probabilistic risk assessment (PRA) using a personal computer (PC) running the Microsoft Windows? operating system. Herein information is provided on the principles used in the construction and operation of Version 6.0 and 7.0 of the SAPHIRE system. This report summarizes the fundamental mathematical concepts of sets and logic, fault trees, and probability. This volume then describes the algorithms used to construct a fault tree and to obtain the minimal cut sets. It gives the formulas used to obtain the probability of the top event from the minimal cut sets, and the formulas for probabilities that apply for various assumptions concerning reparability and mission time. It defines the measures of basic event importance that SAPHIRE can calculate. This volume gives an overview of uncertainty analysis using simple Monte Carlo sampling or Latin Hypercube sampling, and states the algorithms used by this program to generate random basic event probabilities from various distributions. Also covered are enhance capabilities such as seismic analysis, cut set "recovery," end state manipulation, and use of "compound events."

  3. Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.

    Directory of Open Access Journals (Sweden)

    Qiusheng Kong

    Full Text Available Melon (Cucumis melo. L is not only an economically important cucurbitaceous crop but also an attractive model for studying many biological characteristics. Screening appropriate reference genes is essential to reverse transcription quantitative real-time PCR (RT-qPCR, which is key to many studies involving gene expression analysis. In this study, 14 candidate reference genes were selected, and the variations in their expression in roots and leaves of plants subjected to biotic stress, abiotic stress, and plant growth regulator treatment were assessed by RT-qPCR. The stability of the expression of the selected genes was determined and ranked using geNorm and NormFinder. geNorm identified the two most stable genes for each set of conditions: CmADP and CmUBIep across all samples, CmUBIep and CmRPL in roots, CmRAN and CmACT in leaves, CmADP and CmRPL under abiotic stress conditions, CmTUA and CmACT under biotic stress conditions, and CmRAN and CmACT under plant growth regulator treatments. NormFinder determined CmRPL to be the best reference gene in roots and under biotic stress conditions and CmADP under the other experimental conditions. CmUBC2 and CmPP2A were not found to be suitable under many experimental conditions. The catalase family genes CmCAT1, CmCAT2, and CmCAT3 were identified in melon genome and used as target genes to validate the reliability of identified reference genes. The catalase family genes showed the most upregulation 3 days after inoculation with Fusarium wilt in roots, after which they were downregulated. Their levels of expression were significantly overestimated when the unsuitable reference gene was used for normalization. These results not only provide guidelines for the selection of reference genes for gene expression analyses in melons but may also provide valuable information for studying the functions of catalase family genes in stress responses.

  4. Food references and marketing in popular magazines for children and adolescents in New Zealand: a content analysis.

    Science.gov (United States)

    No, Elizabeth; Kelly, Bridget; Devi, Anandita; Swinburn, Boyd; Vandevijvere, Stefanie

    2014-12-01

    Food marketing is recognized as an important factor influencing children's food preferences and consumption. The purpose of this study was to examine the nature and extent of unhealthy food marketing and non-branded food references in magazines targeted at and popular among children and adolescents 10-17 years old in New Zealand. A content analysis was conducted of all food references (branded and non-branded) found in the five magazines with the highest readership among 10-17 year olds, and the three magazines (of which two were already included among the five most popular magazines) targeted to 10-17 year olds. For each of the six magazines, one issue per month (n = 72 issues in total) over a one-year period (December 2012-January 2014) was included. All foods referenced were classified into healthy/unhealthy according to the food-based Ministry of Health classification system. Branded food references (30% of total) were more frequent for unhealthy (43%) compared to healthy (25%) foods. Magazines specifically targeted to children and adolescents contained a significantly higher proportion of unhealthy branded food references (n = 51/71, 72%) compared to the most popular magazines among children and adolescents (n = 133/317, 42%), of which most were targeted to women. 'Snack items' such as chocolates and ice creams were marketed most frequently (n = 104; 36%), while 'vegetables and fruits' were marketed the least frequently (n = 9; 3%). Direct advertisements accounted for 27% of branded food references and 25% of those featured health or nutrition claims. Both branded and non-branded food references were common within magazines targeted at and popular among children and adolescents, and skewed toward unhealthy foods. This raises concerns about the effectiveness of self-regulation in marketing and emphasizes that government regulations are needed in order to curb children's current potential high exposures to unhealthy food marketing. In

  5. Screening suitable reference genes for normalization in reverse transcription quantitative real-time PCR analysis in melon.

    Science.gov (United States)

    Kong, Qiusheng; Yuan, Jingxian; Niu, Penghui; Xie, Junjun; Jiang, Wei; Huang, Yuan; Bie, Zhilong

    2014-01-01

    Melon (Cucumis melo. L) is not only an economically important cucurbitaceous crop but also an attractive model for studying many biological characteristics. Screening appropriate reference genes is essential to reverse transcription quantitative real-time PCR (RT-qPCR), which is key to many studies involving gene expression analysis. In this study, 14 candidate reference genes were selected, and the variations in their expression in roots and leaves of plants subjected to biotic stress, abiotic stress, and plant growth regulator treatment were assessed by RT-qPCR. The stability of the expression of the selected genes was determined and ranked using geNorm and NormFinder. geNorm identified the two most stable genes for each set of conditions: CmADP and CmUBIep across all samples, CmUBIep and CmRPL in roots, CmRAN and CmACT in leaves, CmADP and CmRPL under abiotic stress conditions, CmTUA and CmACT under biotic stress conditions, and CmRAN and CmACT under plant growth regulator treatments. NormFinder determined CmRPL to be the best reference gene in roots and under biotic stress conditions and CmADP under the other experimental conditions. CmUBC2 and CmPP2A were not found to be suitable under many experimental conditions. The catalase family genes CmCAT1, CmCAT2, and CmCAT3 were identified in melon genome and used as target genes to validate the reliability of identified reference genes. The catalase family genes showed the most upregulation 3 days after inoculation with Fusarium wilt in roots, after which they were downregulated. Their levels of expression were significantly overestimated when the unsuitable reference gene was used for normalization. These results not only provide guidelines for the selection of reference genes for gene expression analyses in melons but may also provide valuable information for studying the functions of catalase family genes in stress responses.

  6. Aligning to the sample-specific reference sequence to optimize the accuracy of next-generation sequencing analysis for hepatitis B virus.

    Science.gov (United States)

    Liu, Wen-Chun; Lin, Chih-Peng; Cheng, Chun-Pei; Ho, Cheng-Hsun; Lan, Kuo-Lun; Cheng, Ji-Hong; Yen, Chia-Jui; Cheng, Pin-Nan; Wu, I-Chin; Li, I-Chen; Chang, Bill Chia-Han; Tseng, Vincent S; Chiu, Yen-Cheng; Chang, Ting-Tsung

    2016-01-01

    Hepatitis B virus (HBV) quasispecies are crucial in the pathogenesis of chronic liver disease. Next-generation sequencing (NGS) is powerful for identifying viral quasispecies. To improve mapping quality and single nucleotide variant (SNV) calling accuracy in the NGS analysis of HBV, we compared different mapping references, including the sample-specific reference sequence, same genotype sequences and different genotype sequences, according to the sample. Real Illumina HBV datasets from 86 patients, and simulated datasets from 158 HBV strains in the GenBank database, were used to assess mapping quality. SNV calling accuracy was evaluated using different mapping references to align Real Illumina datasets from a single HBV clone. Using the sample-specific reference sequence as a mapping reference produced the largest number of mappable reads and coverages. With a different genotype mapping reference, the consensus sequence derived from the Real Illumina datasets of the single HBV clone showed 21 false SNV callings in polymerase and surface genes, the regions most divergent between the mapping reference and this HBV clone. A ~6 % coverage of most of these false SNVs was yielded even with a same genotype mapping reference, but none with the sample-specific reference sequence. Using sample-specific reference sequences as a mapping reference in NGS analysis optimized mapping quality and the SNV calling accuracy for HBV quasispecies.

  7. Time series analysis of reference crop evapotranspiration using soft computing techniques for Ganjam District, Odisha, India

    Science.gov (United States)

    Patra, S. R.

    2017-12-01

    Evapotranspiration (ET0) influences water resources and it is considered as a vital process in aridic hydrologic frameworks. It is one of the most important measure in finding the drought condition. Therefore, time series forecasting of evapotranspiration is very important in order to help the decision makers and water system mangers build up proper systems to sustain and manage water resources. Time series considers that -history repeats itself, hence by analysing the past values, better choices, or forecasts, can be carried out for the future. Ten years of ET0 data was used as a part of this study to make sure a satisfactory forecast of monthly values. In this study, three models: (ARIMA) mathematical model, artificial neural network model, support vector machine model are presented. These three models are used for forecasting monthly reference crop evapotranspiration based on ten years of past historical records (1991-2001) of measured evaporation at Ganjam region, Odisha, India without considering the climate data. The developed models will allow water resource managers to predict up to 12 months, making these predictions very useful to optimize the resources needed for effective water resources management. In this study multistep-ahead prediction is performed which is more complex and troublesome than onestep ahead. Our investigation proposed that nonlinear relationships may exist among the monthly indices, so that the ARIMA model might not be able to effectively extract the full relationship hidden in the historical data. Support vector machines are potentially helpful time series forecasting strategies on account of their strong nonlinear mapping capability and resistance to complexity in forecasting data. SVMs have great learning capability in time series modelling compared to ANN. For instance, the SVMs execute the structural risk minimization principle, which allows in better generalization as compared to neural networks that use the empirical risk

  8. Histological and morphometric analysis of dilated cardiomyopathy with special reference to collagen IV expression

    Directory of Open Access Journals (Sweden)

    Parul Jain

    2017-01-01

    Full Text Available Introduction: Collagen distribution alterations are well known in dilated cardiomyopathy. There are also changes in microvasculature along with other histomorphorphological features. Aims and Objectives: To study the histomorphological features of DCM along with their quantitative correlation with LVEF. Alterations in collagen IV distribution pattern and microvasculature in DCM were also evaluated. Materials and Methods: The present study includes 34 right ventricular endomyocardial biopsies, 7 explanted native hearts and 41 autopsy control hearts. Sections were taken from lower half of right interventricular septum and stained for H and E, Masson trichrome and immunohistochemistry for CD34, SMA and Collagen IV to study the histological features, pattern of fibrosis, capillary and arteriolar distribution and collagen IV expression respectively. Morphometric analysis was carried out in all cases and controls using Image analysis software Image pro plus 7 and correlated with left ventricular ejection fraction. Results: The histomorphological changes of DCM include myocyte hypertrophy, nucleomegaly, and interstitial fibrosis. Interfiber fibrosis was the commonest. There was evidence of myocarditis, ischemic change and vessel wall alterations. Considerable alteration in Collagen IV distribution was observed with reduction in intensity and proportion of staining around myocytes quantified using Allred scoring against uniform pericellular staining in controls. Morphometric analysis revealed significant increase in nuclear area, myocyte width, percentage of fibrosis and reduction in capillary myocyte ratio in cases as compared to controls. There was no significant difference in arteriolar density. No significant association was observed between morphometric parameters and LVEF. Conclusion: Histomorphological changes in DCM are non-specific. Quantitation of histological parameters cannot be used to predict the disease progression as there was no

  9. Root Cause Analysis Following an Event at a Nuclear Installation: Reference Manual

    International Nuclear Information System (INIS)

    2015-01-01

    Following an event at a nuclear installation, it is important to determine accurately its root causes so that effective corrective actions can be implemented. As stated in IAEA Safety Standards Series No. SF-1, Fundamental Safety Principles: “Processes must be put in place for the feedback and analysis of operating experience”. If this process is completed effectively, the probability of a similar event occurring is significantly reduced. Guidance on how to establish and implement such a process is given in IAEA Safety Standards Series No. NS-G-2.11, A System for the Feedback of Experience from Events in Nuclear Installations. To cater for the diverse nature of operating experience events, several different root cause analysis (RCA) methodologies and techniques have been developed for effective investigation and analysis. An event here is understood as any unanticipated sequence of occurrences that results in, or potentially results in, consequences to plant operation and safety. RCA is not a topic uniquely relevant to event investigators: knowledge of the concepts enhances the learning characteristics of the whole organization. This knowledge also makes a positive contribution to nuclear safety and helps to foster a culture of preventing event occurrence. This publication allows organizations to deepen their knowledge of these methodologies and techniques and also provides new organizations with a broad overview of the RCA process. It is the outcome of a coordinated effort involving the participation of experts from nuclear organizations, the energy industry and research centres in several Member States. This publication also complements IAEA Services Series No. 10, PROSPER Guidelines: Guidelines for Peer Review and for Plant Self- Assessment of Operational Experience Feedback Process, and is intended to form part of a suite of publications developing the principles set forth in these guidelines. In addition to the information and description of RCA

  10. Root Cause Analysis Following an Event at a Nuclear Installation: Reference Manual. Companion CD

    International Nuclear Information System (INIS)

    2015-01-01

    Following an event at a nuclear installation, it is important to determine accurately its root causes so that effective corrective actions can be implemented. As stated in IAEA Safety Standards Series No. SF-1, Fundamental Safety Principles: “Processes must be put in place for the feedback and analysis of operating experience”. If this process is completed effectively, the probability of a similar event occurring is significantly reduced. Guidance on how to establish and implement such a process is given in IAEA Safety Standards Series No. NS-G-2.11, A System for the Feedback of Experience from Events in Nuclear Installations. To cater for the diverse nature of operating experience events, several different root cause analysis (RCA) methodologies and techniques have been developed for effective investigation and analysis. An event here is understood as any unanticipated sequence of occurrences that results in, or potentially results in, consequences to plant operation and safety. RCA is not a topic uniquely relevant to event investigators: knowledge of the concepts enhances the learning characteristics of the whole organization. This knowledge also makes a positive contribution to nuclear safety and helps to foster a culture of preventing event occurrence. This publication allows organizations to deepen their knowledge of these methodologies and techniques and also provides new organizations with a broad overview of the RCA process. It is the outcome of a coordinated effort involving the participation of experts from nuclear organizations, the energy industry and research centres in several Member States. This publication also complements IAEA Services Series No. 10, PROSPER Guidelines: Guidelines for Peer Review and for Plant Self- Assessment of Operational Experience Feedback Process, and is intended to form part of a suite of publications developing the principles set forth in these guidelines. In addition to the information and description of RCA

  11. Comparative characteristic of concentration units relating to reference materials for gas chromatography analysis of hydrocarbon samples

    Directory of Open Access Journals (Sweden)

    S. A. Arystanbekova

    2014-01-01

    Full Text Available Application of a method of absolute calibration relating to gas chromatography analysis of liquid hydrocarbon samples is considered. It is shown for this task that both from theoretical, and practical points of view the optimum concentration unit is mass (not molar fraction. Information on average molar mass of the analyzed sample is necessary for the determination of analytes in liquid hydrocarbon samples in terms of mole fraction. Meanwhile, the normative documents of rather high rank (ASTM, ISO, GOST, GOST R concerning methods of the determination of average molar weight of samples of such a kind are absent.

  12. Superconductors, analysis and applications, with special reference to the utilisation of bulk (Re)BCO materials

    Energy Technology Data Exchange (ETDEWEB)

    Coombs, T.A., E-mail: tac1000@cam.ac.u [University of Cambridge, Department of Engineering, Trumpington Street, Cambridge CB2 1PZ (United Kingdom)

    2010-11-01

    The Electrical Power and Energy Conversion (EPEC) superconductivity group at Cambridge University has been working on the application of superconductivity to large scale devices. This work is taking place over a range of areas which cover FCLs, motors and generators, SMES, accelerator magnets and MRI. The research is underpinned by advanced modelling techniques using both pure Critical State models and E-J models to analyse the behaviour of the superconductors. As part of the device design we are concentrating on the analysis of AC losses in complicated geometries such as are found in motor windings and the magnetisation of bulk superconductors to enable their full potential to be realised. We are interested in the full range of high-temperature superconductors and have measured and predicted the performance of YBCO, MgB{sub 2} and BSCCO at a range of temperatures and in wire, tape and bulk forms. This paper concentrates on recent work which includes: modelling of coils using formulations based on H and A. A critical state model for the analysis of coils in SMES; crossed field effects in bulk superconductors; a magnetic model together with experimental results which explain and describe the method of flux pumping whereby a bulk superconductor can be magnetised to a high flux density using a repeatedly applied field of low flux density and finally a new configuration for MRI magnets

  13. New Approaches to Transport Project Assessment: Reference Scenario Forecasting and Quantitative Risk Analysis

    DEFF Research Database (Denmark)

    Salling, Kim Bang

    2010-01-01

    This presentation sets out a new methodology for examining the uncertainties relating to transport decision making based on infrastructure appraisals. Traditional transport infrastructure projects are based upon cost-benefit analyses in order to appraise the projects feasibility. Recent research ....... Additionally, the handling of uncertainties is supplemented by making use of the principle of Optimism Bias, which depicts the historical tendency of overestimating transport related benefits (user demands i.e. travel time savings) and underestimating investment costs.......This presentation sets out a new methodology for examining the uncertainties relating to transport decision making based on infrastructure appraisals. Traditional transport infrastructure projects are based upon cost-benefit analyses in order to appraise the projects feasibility. Recent research...... however has proved that the point estimates derived from such analyses are embedded with a large degree of uncertainty. Thus, a new scheme was proposed in terms of applying quantitative risk analysis (QRA) and Monte Carlo simulation in order to represent the uncertainties within the cost-benefit analysis...

  14. A reference methylome database and analysis pipeline to facilitate integrative and comparative epigenomics.

    Directory of Open Access Journals (Sweden)

    Qiang Song

    Full Text Available DNA methylation is implicated in a surprising diversity of regulatory, evolutionary processes and diseases in eukaryotes. The introduction of whole-genome bisulfite sequencing has enabled the study of DNA methylation at a single-base resolution, revealing many new aspects of DNA methylation and highlighting the usefulness of methylome data in understanding a variety of genomic phenomena. As the number of publicly available whole-genome bisulfite sequencing studies reaches into the hundreds, reliable and convenient tools for comparing and analyzing methylomes become increasingly important. We present MethPipe, a pipeline for both low and high-level methylome analysis, and MethBase, an accompanying database of annotated methylomes from the public domain. Together these resources enable researchers to extract interesting features from methylomes and compare them with those identified in public methylomes in our database.

  15. Elemental analysis of biological materials. Current problems and techniques with special reference to trace elements

    International Nuclear Information System (INIS)

    1980-01-01

    Selected techniques were reviewed for the assay of trace and minor elements in biological materials. Other relevant information is also presented on the need for such analyses, sampling, sample preparation and analytical quality control. In order to evaluate and compare the applicability of the various analytical techniques on a meaningful and objective basis, the materials chosen for consideration were intended to be typical of a wide range of biological matrics of different elemental compositions, namely Bowen's kale, representing a plant material, and NBS bovine liver, IAEA animal muscle, and blood serum, representing animal tissues. The subject is reviewed under the following headings: on the need for trace element analyses in the life sciences (4 papers); sampling and sample preparation for trace element analysis (2 papers); analytical techniques for trace and minor elements in biological materials (7 papers); analytical quality control (2 papers)

  16. Preparation and evaluation of reference materials for accountancy analysis. (2) Evaluation results

    International Nuclear Information System (INIS)

    Sumi, Mika; Abe, Katsuo; Kageyama, Tomio; Nakazawa, Hiroaki; Takamatsu, Mai; Kacchi, Tomokazu; Murakami, Toshiki; Ai, Hironobu

    2009-01-01

    Destructive analysis for accountancy at nuclear fuel facilities should attain international target values for measurement uncertainties in safeguarding nuclear materials (ITVs). Since measurement uncertainties of isotope dilution mass spectrometry depend on uncertainties of spikes (standard materials) used, utilizing highly reliable standard material is essential. The LSD spikes prepared under collaboration work with JAEA and JNFL has different Pu/U ratio and smaller nuclear material in a spike compared with the LSD spikes used a safeguard laboratories, and the value of Pu which separated and purified from MOX and used as raw material for one of the LSD spike prepared at JAEA were measured at JAEA. Uncertainties of the prepared LSD spikes and the measurement results of actual samples with these LSD spikes were evaluated based on ISO-GUM and compared with ITVs. (author)

  17. A new RNASeq-based reference transcriptome for sugar beet and its application in transcriptome-scale analysis of vernalization and gibberellin responses

    Science.gov (United States)

    2012-01-01

    Background Sugar beet (Beta vulgaris sp. vulgaris) crops account for about 30% of world sugar. Sugar yield is compromised by reproductive growth hence crops must remain vegetative until harvest. Prolonged exposure to cold temperature (vernalization) in the range 6°C to 12°C induces reproductive growth, leading to bolting (rapid elongation of the main stem) and flowering. Spring cultivation of crops in cool temperate climates makes them vulnerable to vernalization and hence bolting, which is initiated in the apical shoot meristem in processes involving interaction between gibberellin (GA) hormones and vernalization. The underlying mechanisms are unknown and genome scale next generation sequencing approaches now offer comprehensive strategies to investigate them; enabling the identification of novel targets for bolting control in sugar beet crops. In this study, we demonstrate the application of an mRNA-Seq based strategy for this purpose. Results There is no sugar beet reference genome, or public expression array platforms. We therefore used RNA-Seq to generate the first reference transcriptome. We next performed digital gene expression profiling using shoot apex mRNA from two sugar beet cultivars with and without applied GA, and also a vernalized cultivar with and without applied GA. Subsequent bioinformatics analyses identified transcriptional changes associated with genotypic difference and experimental treatments. Analysis of expression profiles in response to vernalization and GA treatment suggested previously unsuspected roles for a RAV1-like AP2/B3 domain protein in vernalization and efflux transporters in the GA response. Conclusions Next generation RNA-Seq enabled the generation of the first reference transcriptome for sugar beet and the study of global transcriptional responses in the shoot apex to vernalization and GA treatment, without the need for a reference genome or established array platforms. Comprehensive bioinformatic analysis identified

  18. [Possible applications of the KAMEDIN teleradiology system with special reference to an economic analysis].

    Science.gov (United States)

    Lehmann, K J; Walz, M; Bolte, R; Georgi, M; Schinkmann, M; Busch, C

    1997-04-01

    Different concepts and applications of teleradiology systems have been realised. However, their cost-effectiveness is still questionable. Therefore, a cost-benefit analysis of three different scenarios of the new teleradiology system Kamedin (Kooperatives Arbeiten und rechnergestützte Medizinische Diagnostik auf innovativen Netzen der Deutschen Telekom) was performed. CT examinations were transmitted from an Advantage Windows (GE) workstation to a Kamedin workstation using DICOM 3 protocol. Afterwards a teleconference was established with a Kamedin workstation in the intensive care unit within the hospital via FDDI/Ethernet, with an external workstation in a radiology department 6 km away via ISDN and with a Kamedin PC located with radiologist on duty 22 km away via ISDN. On average, 36 CT slices per patient were transferred. A break-even analysis was performed with respect to costs of hardware, software, support, use of ISDN and staff, as well as benefits like the decrease in transportation or film documentation costs. Owing to the different reductions in transportation costs, two applications (intensive care unit and external PC) showed a break-even of 1817 and 528 teleconferences/year, respectively. Further optimisation of cost-effectiveness is possible on condition that existing hardware can be used and an automatic data transfer without staff control is available. When all optimisation factors were combined, the break-even decreased to a minimum of 167 and 77 teleconferences/ year, respectively. Teleconferences with high image quality can be set up between workstations and PCs using the Kamedin system. Depending on the possible decrease in transportation costs, teleconferencing is cost-effective under certain conditions. Teleradiology has additional advantages, such as the acceleration and optimisation of patient management.

  19. Noticing 'us' and 'them' constructions: The pedagogical implications of a critical discourse analysis of referring in political discourse

    Directory of Open Access Journals (Sweden)

    Susan Brokensha

    2011-09-01

    Full Text Available In the past decade, there have been a number of discourse analyses of the political in South Africa such as Botha’s (2001 study of deictic expressions in a speech by Thabo Mbeki, Moodley’s (2006 discursive analysis of the South African government’s Information and Communication Technologies, poverty, and development discourse, and Moon’s (2006 investigation into the discursive construction of narratives generated during the Truth and Reconciliation Commission. However, how such studies may be exploited to foster students’ critical thinking about language use in a variety of communicative contexts has not been explored in much detail. Within the framework of Critical Discourse Analysis (CDA, the aim of this paper is to examine the phenomenon of referring in a political speech by Robert Mugabe, focusing specifically on his use of spatial, temporal, and social indexicals to construct us versus them territories. Next, the constructivist principles that may be adopted to assist second-year Linguistics students in developing an awareness that referring is not a neutral phenomenon are identified.

  20. Manned space flight nuclear system safety. Volume 3: Reactor system preliminary nuclear safety analysis. Part 1: Reference Design Document (RDD)

    Science.gov (United States)

    1972-01-01

    The Reference Design Document, of the Preliminary Safety Analysis Report (PSAR) - Reactor System provides the basic design and operations data used in the nuclear safety analysis of the Rector Power Module as applied to a Space Base program. A description of the power module systems, facilities, launch vehicle and mission operations, as defined in NASA Phase A Space Base studies is included. Each of two Zirconium Hydride Reactor Brayton power modules provides 50 kWe for the nominal 50 man Space Base. The INT-21 is the prime launch vehicle. Resupply to the 500 km orbit over the ten year mission is provided by the Space Shuttle. At the end of the power module lifetime (nominally five years), a reactor disposal system is deployed for boost into a 990 km high altitude (long decay time) earth orbit.

  1. Radiochemical neutron-activation analysis of uncertified ultra-trace rare earth elements in two biological certified reference materials

    International Nuclear Information System (INIS)

    Cao, Lei; Tian, Weizhi; Ni, Bangfa; Wang, Pingsheng; Zhang, Yangmei

    2002-01-01

    Radiochemical neutron activation analysis (RNAA) has been used for the determination of eight rare earth elements (La, Ce, Nd, Sm, Eu, Tb, Yb, and Lu) in two Chinese certified reference materials (CRM), GBW 08503 (wheat powder) and GBW 09101 (human hair). These determinations are important for possible certification of the above mentioned ultra-trace elements, so far not certified. A simple one-step (REE)F 3 precipitation was used. Chemical yields were determined for all relevant elements by means of tracer experiments. The two CRM were also analyzed by inductively coupled plasma-mass spectrometry (ICP-MS) to compare the merits and drawbacks of these two major trace analytical techniques for these particular elements. RNAA was proven to be a reliable technique for ultra-trace analysis, especially in the certification of some ultra-trace elements. (orig.)

  2. Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)

    OpenAIRE

    Zhang, Xin; Xu, Zhi-Chao; Xu, Jiang; Ji, Ai-Jia; Luo, Hong-Mei; Song, Jing-Yuan; Sun, Chao; Hu, Yuan-Lei; Chen, Shi-Lin

    2016-01-01

    Background Quantitative real-time reverse transcription PCR (qRT-PCR) requires a stable internal control to avoid misinterpretation of data or errors for gene expression normalization. However, there are still no validated reference genes for stable internal control in Poria cocos (Schw.) Wolf (Fuling). This study aims to validate the reference genes of P. cocos. Methods This study firstly collected the 14 candidate reference genes by BLASTP from the genome of P. cocos for qRT-PCR analysis to...

  3. Suitable reference genes for the analysis of direct hyperplasia in mice

    International Nuclear Information System (INIS)

    Takagi, Soichi; Ohashi, Kazuo; Utoh, Rie; Tatsumi, Kohei; Shima, Midori; Okano, Teruo

    2008-01-01

    The liver is capable of undergoing a proliferative growth, known as direct hyperplasia, in which the naive liver increases in size due to stimulation with primary mitogens. To produce accurate gene expression data, housekeeping genes (HKGs) that are stably expressed need to be determined. In the present study, liver regeneration was promoted via the direct hyperplasia mode by inducing mice with 1,4-bis[2-(3,5-dichloropyridyloxy)]benzene. Gene expression levels of nine commonly used HKGs were analyzed in the liver of different timing during the regeneration. The stability of gene expression was assessed using two different analysis programs, geNorm and NormFinder. Using these analyses, we identified that PPIA and RPL4 showed the most stable expression regardless of the status of the liver regeneration. In conclusion, the present study demonstrated that the use of PPIA and RPL4 were the most optimal in providing reliable normalization of gene expression when assessing liver regeneration attributed to direct hyperplasia.

  4. Usability and use reference in the social network facebook: a netnographic analysis of technological users

    Directory of Open Access Journals (Sweden)

    Naiara Silva Ferreira

    2015-10-01

    Full Text Available This article presents a study about the preference of use in virtual social networks, using Facebook as object of study, to identify the motivational factors for the usability of this technology platform. The social network Facebook has been chosen to present a technological scenario of high sociability and virtual interaction. The methodology used was the netnography, being made through the collection of discussions in North American sites of news and forums online, where there is a large critical user participation on the internet, about the gains and frustrations in this context. The content analysis was performed comparing the categories of users found in the literature about values that motivate consumer technology, describing the hedonic, social, utilitarian values and perceptions of risk in consumption when related to lack of privacy. The results show two main groups of users of this technology and 7 subgroups. Therefore, the contribution of the study is that the formation of these groups may reflect technological usability of user groups around the world. The study also brings to the discussion issues related to the behaviors of the users of virtual networks which can be useful for businesses and their relationships with consumers and also the development of new knowledge from such criticism and demands that digital consumers expose about the technologies.

  5. Hawaii demand-side management resource assessment. Final report, Reference Volume 1: Building prototype analysis

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-04-01

    This report provides a detailed description of, and the baseline assumptions and simulation results for, the building prototype simulations conducted for the building types designated in the Work Plan for Demand-side Management Assessment of Hawaii`s Demand-Side Resources (HES-4, Phase 2). This report represents the second revision to the initial building prototype description report provided to DBEDT early in the project. Modifications and revisions to the prototypes, based on further calibration efforts and on comments received from DBEDT Staff have been incorporated into this final version. These baseline prototypes form the basis upon which the DSM measure impact estimates and the DSM measure data base were developed for this project. This report presents detailed information for each of the 17 different building prototypes developed for use with the DOE-21E program (23 buildings in total, including resorts and hotels defined separately for each island) to estimate the impact of the building technologies and measures included in this project. The remainder of this section presents some nomenclature and terminology utilized in the reports, tables, and data bases developed from this project to denote building type and vintage. Section 2 contains a more detailed discussion of the data sources, the definition of the residential sector building prototypes, and results of the DOE-2 analysis. Section 3 provides a similar discussion for the commercial sector. The prototype and baseline simulation results are presented in a separate section for each building type. Where possible, comparison of the baseline simulation results with benchmark data from the ENERGY 2020 model or other demand forecasting models specific to Hawaii is included for each building. Appendix A contains a detailed listing of the commercial sector baseline indoor lighting technologies included in the existing and new prototypes by building type.

  6. Analysis of data relative to the update of diagnostic reference levels in radiology and nuclear medicine. 2011-2012 review

    International Nuclear Information System (INIS)

    2014-01-01

    Applying the Order of 24 October 2011 on diagnostic reference levels, departments of radiology and nuclear medicine must send a sample of 'patient' dosimetric data to the IRSN each year. The results of the analysis of dosimetric data performed between the 1 January 2011 and the 31 December 2012 presented in this report should enable the authority to define the needs for updating regulations. Professional involvement in DRLs improved globally over the 2011-2012 period but is heterogeneous according to the imaging area considered. The participation of conventional radiology professionals is still low, with less than 30% against over 75% in CT and 85% in nuclear medicine. Data collection in pediatrics, considering all the fields of medical imaging, remains extremely limited. This shows almost no dose assessment for children by imaging departments, and has the effect of not allowing authorities to provide professionals with DRLs representative of pediatric practices. The analysis of radiology doses and nuclear medicine administered activities by IRSN shows an overall decrease of statistical indicators on which DRLs are indexed. These results lead to proposals for updating reference values for a large number of examinations. In addition to the analysis of data collected for examinations currently mentioned in regulatory texts, IRSN recommends to update DRLs in a more general way by changing the strategy for collecting and updating pediatric DRLs, by including interventional radiology - specialty in which the radiation protection presents a major challenge - by introducing a more ambitious indicator than the 75. percentile in conventional radiology and nuclear medicine - the 25. percentile statistical indicator, and by taking into account new technologies inducing additional exposures to the patient as CT-scan associated with the PET. (authors)

  7. Development of a Reference Image Collection Library for Histopathology Image Processing, Analysis and Decision Support Systems Research.

    Science.gov (United States)

    Kostopoulos, Spiros; Ravazoula, Panagiota; Asvestas, Pantelis; Kalatzis, Ioannis; Xenogiannopoulos, George; Cavouras, Dionisis; Glotsos, Dimitris

    2017-06-01

    Histopathology image processing, analysis and computer-aided diagnosis have been shown as effective assisting tools towards reliable and intra-/inter-observer invariant decisions in traditional pathology. Especially for cancer patients, decisions need to be as accurate as possible in order to increase the probability of optimal treatment planning. In this study, we propose a new image collection library (HICL-Histology Image Collection Library) comprising 3831 histological images of three different diseases, for fostering research in histopathology image processing, analysis and computer-aided diagnosis. Raw data comprised 93, 116 and 55 cases of brain, breast and laryngeal cancer respectively collected from the archives of the University Hospital of Patras, Greece. The 3831 images were generated from the most representative regions of the pathology, specified by an experienced histopathologist. The HICL Image Collection is free for access under an academic license at http://medisp.bme.teiath.gr/hicl/ . Potential exploitations of the proposed library may span over a board spectrum, such as in image processing to improve visualization, in segmentation for nuclei detection, in decision support systems for second opinion consultations, in statistical analysis for investigation of potential correlations between clinical annotations and imaging findings and, generally, in fostering research on histopathology image processing and analysis. To the best of our knowledge, the HICL constitutes the first attempt towards creation of a reference image collection library in the field of traditional histopathology, publicly and freely available to the scientific community.

  8. Method applied to the background analysis of energy data to be considered for the European Reference Life Cycle Database (ELCD).

    Science.gov (United States)

    Fazio, Simone; Garraín, Daniel; Mathieux, Fabrice; De la Rúa, Cristina; Recchioni, Marco; Lechón, Yolanda

    2015-01-01

    Under the framework of the European Platform on Life Cycle Assessment, the European Reference Life-Cycle Database (ELCD - developed by the Joint Research Centre of the European Commission), provides core Life Cycle Inventory (LCI) data from front-running EU-level business associations and other sources. The ELCD contains energy-related data on power and fuels. This study describes the methods to be used for the quality analysis of energy data for European markets (available in third-party LC databases and from authoritative sources) that are, or could be, used in the context of the ELCD. The methodology was developed and tested on the energy datasets most relevant for the EU context, derived from GaBi (the reference database used to derive datasets for the ELCD), Ecoinvent, E3 and Gemis. The criteria for the database selection were based on the availability of EU-related data, the inclusion of comprehensive datasets on energy products and services, and the general approval of the LCA community. The proposed approach was based on the quality indicators developed within the International Reference Life Cycle Data System (ILCD) Handbook, further refined to facilitate their use in the analysis of energy systems. The overall Data Quality Rating (DQR) of the energy datasets can be calculated by summing up the quality rating (ranging from 1 to 5, where 1 represents very good, and 5 very poor quality) of each of the quality criteria indicators, divided by the total number of indicators considered. The quality of each dataset can be estimated for each indicator, and then compared with the different databases/sources. The results can be used to highlight the weaknesses of each dataset and can be used to guide further improvements to enhance the data quality with regard to the established criteria. This paper describes the application of the methodology to two exemplary datasets, in order to show the potential of the methodological approach. The analysis helps LCA

  9. Analysis of micronucleated erythrocytes in heron nestlings from reference and impacted sites in the Ebro basin (N.E. Spain)

    International Nuclear Information System (INIS)

    Quiros, Laia; Ruiz, Xavier; Sanpera, Carolina; Jover, Lluis; Pina, Benjamin

    2008-01-01

    The frequency of micronuclei (MN) in peripheral erythrocytes was tested for 59 heron nestlings (Ardea purpurea, Egretta garzetta and Bubulcus ibis) sampled at two areas (polluted and reference) on the River Ebro (NE Spain) and at its Delta during Spring 2006. Flow-cytometry analysis revealed higher (three- to six-fold) MN counts in samples from the most polluted site relative to samples from the reference area. Samples from the Delta showed intermediate values. Age, morphometric parameters (weight, tarsus size and bill-head length) and maturation status showed no significant differences among the different populations for each species; nor were they correlated with MN levels. The data suggest that elevated levels of MN in chicks in impacted areas reflected the chemical pollution of their nesting sites. The use of nestlings for this assay appears to be a convenient, non-destructive method to assess the impact of pollution in natural bird populations. - Frequency of micronucleated erythrocytes in peripheral blood of waterbird nestlings correlates with chemical pollution loads in their nesting sites

  10. Identification and comprehensive evaluation of reference genes for RT-qPCR analysis of host gene-expression in Brassica juncea-aphid interaction using microarray data.

    Science.gov (United States)

    Ram, Chet; Koramutla, Murali Krishna; Bhattacharya, Ramcharan

    2017-07-01

    Brassica juncea is a chief oil yielding crop in many parts of the world including India. With advancement of molecular techniques, RT-qPCR based study of gene-expression has become an integral part of experimentations in crop breeding. In RT-qPCR, use of appropriate reference gene(s) is pivotal. The virtue of the reference genes, being constant in expression throughout the experimental treatments, needs to be validated case by case. Appropriate reference gene(s) for normalization of gene-expression data in B. juncea during the biotic stress of aphid infestation is not known. In the present investigation, 11 reference genes identified from microarray database of Arabidopsis-aphid interaction at a cut off FDR ≤0.1, along with two known reference genes of B. juncea, were analyzed for their expression stability upon aphid infestation. These included 6 frequently used and 5 newly identified reference genes. Ranking orders of the reference genes in terms of expression stability were calculated using advanced statistical approaches such as geNorm, NormFinder, delta Ct and BestKeeper. The analysis suggested CAC, TUA and DUF179 as the most suitable reference genes. Further, normalization of the gene-expression data of STP4 and PR1 by the most and the least stable reference gene, respectively has demonstrated importance and applicability of the recommended reference genes in aphid infested samples of B. juncea. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

  11. Summary report of second research coordination meeting on development of a reference database for ion beam analysis

    International Nuclear Information System (INIS)

    Vickridge, Ian; Schwerer, Otto

    2007-07-01

    Highlights of the 2nd Research Coordination Meeting (RCM) are given with respect to the progress achieved in the first 1 1/2 years of the Co-ordinated Research Project (CRP) on Development of a Reference Database for Ion Beam Analysis. Participants presented the results of their work to date, and identified and assigned key tasks required to ensure that the final output of the CRP is achieved. In addition, a number of lively and productive discussions took place concerning technical issues such as accelerator energy calibration, error reporting, accuracy of the existing IBANDL and EXFOR datasets for IBA, and procedures for producing recommended cross-section data. The main conclusions as well as lists of actions and tasks are presented in this report. (author)

  12. High resolution chromosome analysis and fluorescence in situ hybridization in patients referred for Prader-Willi or Angelman syndrome

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-05-08

    Laboratory testing is helpful in the evaluation of patients suspected to have either Prader-Willi syndrome (PWS) or Angelman syndrome (AS) because most of the patients have recognizable cytogenetic deletions of 15q11q13. Maternal uniparental disomy of chromosome 15, identified by molecular genetic techniques, is found in about 20 to 25% of PWS patients. Paternal uniparental disomy of chromosome 15 is seen in 2 to 3% of AS patients. Thus, PWS and AS represent the first examples in humans of genetic imprinting or the differential expression of genetic information depending on the parental origin. Herein, I report our experience with FISH and high resolution chromosome analysis in patients referred to confirm or rule out PWS or AS. 10 refs., 1 tab.

  13. Determination of rare earth and uranium in reference biological materials certified by the method of neutron activation analysis

    International Nuclear Information System (INIS)

    Pereira, Lais H.P.; Saiki, Mitiko

    2013-01-01

    The objective of this study was to evaluate the precision and accuracy of the determinations of Sc, La, Ce, Nd, Sm, Eu, Tb, Yb, Lu and U in certified reference materials (CRMs). To solve the problem of interference from fission products of U in the determination of lanthanides were obtained correction factors for this interference for 140 La, 141 Ce, 143 Ce, 153 Sm and 147 Nd. The experimental procedure of Neutron Activation Analysis consisted of irradiating aliquots of each of the CRMs with synthetic standards of elements under thermal neutron flux of the IEA-R1 nuclear reactor, followed by gamma-ray spectrometry using a high-resolution hyperpurity GE detector. The analyzes of CRMs indicate good accuracy and precision of results, demonstrating the feasibility of applying of established procedure in NAA of elements studied in organic vegetable matrices

  14. 3. research coordination meeting on development of a reference data base for ion beam analysis. Summary report

    International Nuclear Information System (INIS)

    Abriola, D.; Vickridge, I.

    2009-12-01

    Highlights of the third and last Research Coordination Meeting are given with respect to the progress achieved in the Co-ordinated Research Project on Development of a Reference Database for Ion Beam Analysis. The meeting took place at the IAEA headquarters in Vienna from 27 March to 3 April 2009. Participants presented the results of their work and identified and assigned key tasks in pursuance of the final output of the CRP, in particular the update of the IBANDL library and the drafting of the final Technical Report of the CRP. In addition, a number of productive discussions took place concerning issues such as measurements, assessments, evaluations, benchmarks and recommendations. The main conclusions as well as lists of responsibilities and tasks towards the production of the final report are presented. (author)

  15. Tasmania's Reproductive Health (Access to Terminations) Act 2013: An analysis of conscientious objection to abortion and the "obligation to refer".

    Science.gov (United States)

    Sifris, Ronli

    2015-06-01

    This article focuses on Tasmania's Reproductive Health (Access to Terminations) Act 2013, which decriminalises abortion in that State. The article first provides an overview of the Tasmanian legislation, comparing it with Victoria's Abortion Law Reform Act 2008. It then provides a more in-depth analysis of a doctor's right to "conscientious objection" and the requirement in both Acts of an "obligation to refer". The article concludes that ultimately, as a democratic society, it is important that both a woman's right to terminate a pregnancy and a doctor's right to freedom of conscience is respected. Where these rights conflict, as is the case when a doctor with a conscientious objection to abortion is confronted with a patient who seeks information about abortion, they must be balanced. The Victorian and Tasmanian Acts represent a considered and reasonable approach to balancing the rights at issue.

  16. Statistical Analysis of a Round-Robin Measurement Survey of Two Candidate Materials for a Seebeck Coefficient Standard Reference Material

    Directory of Open Access Journals (Sweden)

    Lu, Z. Q. J.

    2009-01-01

    Full Text Available In an effort to develop a Standard Reference Material (SRM™ for Seebeck coefficient, we have conducted a round-robin measurement survey of two candidate materials—undoped Bi2Te3 and Constantan (55 % Cu and 45 % Ni alloy. Measurements were performed in two rounds by twelve laboratories involved in active thermoelectric research using a number of different commercial and custom-built measurement systems and techniques. In this paper we report the detailed statistical analyses on the interlaboratory measurement results and the statistical methodology for analysis of irregularly sampled measurement curves in the interlaboratory study setting. Based on these results, we have selected Bi2Te3 as the prototype standard material. Once available, this SRM will be useful for future interlaboratory data comparison and instrument calibrations.

  17. Homogeneity tests and certification analyses of the IRANT coal fly ash reference material ECO by instrumental neutron activation analysis

    International Nuclear Information System (INIS)

    Kucera, J.; Soukal, L.

    1989-01-01

    Instrumental neutron activation analysis technique was used for homogeneity tests and certification analyses of the coal fly ash reference material ECO prepared at the Institute of Radioecology and Applied Nuclear Techniques (IRANT), Kosice, Czechoslovakia. The relative standard deviations due to inhomogeneity were found to be < 3% for 19 elements with sample weights about 50 mg. The results of determination of the elements Al,As,Ba,Ca,Ce,Co,Cr,Cs,Dy,Eu,Fe,Ga,Hf,In,K,La,Mn,Mo,Na,Nd,Ni,Rb, Sb,Sc,Sm,Sr,Ta,Th,Ti,U,V,W and Zn were compared with the IRANT certified or information values. Interference was made on the reliability of the IRANT specified values for the element contents. (author) 7 refs.; 6 tabs

  18. Collaborative analysis for certification of zirconium and zirconium base alloy reference materials JAERI-Z11 to Z16

    International Nuclear Information System (INIS)

    1985-03-01

    The second Sub-Committee on Zircaloy Analysis was organized in April 1978, under the Committee on Analytical Chemistry on Nuclear Fuels and Reactor Materials, JAERI, for the renewal of zirconium and zirconium base alloy certified reference materials (CRMs). The Sub-Committee carried out collaborative analysis among 13 participating laboratories for the certification of the CRMs, JAERI-Z11 to Z18, after development, improvement and evaluation of analytical methods during the period of May 1978 to June 1982. As the result of the collaborative analysis, the certified value was given for 18 elements (Sn, Fe, Ni, Cr, B, Cd, U, Cu, Co, Mn, Pb, Al, Ti, Si, Mo, W, Hf, C) in the CRMs. The first part of this report includes general discussion, the second part principles of certification, the third part development and verification of analytical methods, and the fourth part evaluation of analytical results on 17 elements. Preparation of Z11 to Z18, and certification for carbon in JAERI-Z17 and Z18 were reported separately in JAERI-M 83-241 and M 83-035, respectively. (author)

  19. Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain

    Science.gov (United States)

    Moreno, Luisa Z; Miraglia, Fabiana; Loureiro, Ana P; Kremer, Frederico S; Eslabao, Marcus R; Dellagostin, Odir A; Lilenbaum, Walter; Vasconcellos, Silvio A; Heinemann, Marcos B; Moreno, Andrea M

    2018-01-01

    Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species. PMID:29538491

  20. Genomic characterisation of Leptospira inadai serogroup Lyme isolated from captured rat in Brazil and comparative analysis with human reference strain.

    Science.gov (United States)

    Moreno, Luisa Z; Miraglia, Fabiana; Loureiro, Ana P; Kremer, Frederico S; Eslabao, Marcus R; Dellagostin, Odir A; Lilenbaum, Walter; Vasconcellos, Silvio A; Heinemann, Marcos B; Moreno, Andrea M

    2018-03-12

    Leptospira inadai is classified as a species of the Leptospira intermediate group that has been poorly studied due to its apparent insignificance to human and animal health. Nevertheless, over the last two decades the species has been described in human cases in India and in carrier animals in Ecuador. Here, we present the first identification and genomic characterisation of L. inadai serogroup Lyme isolated from captured rodent in Brazil. Even though the M34/99 strain was not pathogenic for hamsters, it was able to establish renal colonisation. The M34/99 strain presented high similarity with L. inadai serogroup Lyme human reference indicating that animal strain could also infect humans, although it does not represent high risk of severe disease. An extrachromosomal sequence was also identified in M34/99 strain and presented high identity with previously described L. inadai phage LinZ_10, suggesting that phage-like extrachromosomal sequence may be another feature of this understudied species.

  1. Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE), Version 5.0: Integrated Reliability and Risk Analysis System (IRRAS) reference manual. Volume 2

    International Nuclear Information System (INIS)

    Russell, K.D.; Kvarfordt, K.J.; Skinner, N.L.; Wood, S.T.; Rasmuson, D.M.

    1994-07-01

    The Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) refers to a set of several microcomputer programs that were developed to create and analyze probabilistic risk assessments (PRAs), primarily for nuclear power plants. The Integrated Reliability and Risk Analysis System (IRRAS) is a state-of-the-art, microcomputer-based probabilistic risk assessment (PRA) model development and analysis tool to address key nuclear plant safety issues. IRRAS is an integrated software tool that gives the use the ability to create and analyze fault trees and accident sequences using a microcomputer. This program provides functions that range from graphical fault tree construction to cut set generation and quantification to report generation. Version 1.0 of the IRRAS program was released in February of 1987. Since then, many user comments and enhancements have been incorporated into the program providing a much more powerful and user-friendly system. This version has been designated IRRAS 5.0 and is the subject of this Reference Manual. Version 5.0 of IRRAS provides the same capabilities as earlier versions and ads the ability to perform location transformations, seismic analysis, and provides enhancements to the user interface as well as improved algorithm performance. Additionally, version 5.0 contains new alphanumeric fault tree and event used for event tree rules, recovery rules, and end state partitioning

  2. Automated ultrasound edge-tracking software comparable to established semi-automated reference software for carotid intima-media thickness analysis.

    Science.gov (United States)

    Shenouda, Ninette; Proudfoot, Nicole A; Currie, Katharine D; Timmons, Brian W; MacDonald, Maureen J

    2017-04-26

    Many commercial ultrasound systems are now including automated analysis packages for the determination of carotid intima-media thickness (cIMT); however, details regarding their algorithms and methodology are not published. Few studies have compared their accuracy and reliability with previously established automated software, and those that have were in asymptomatic adults. Therefore, this study compared cIMT measures from a fully automated ultrasound edge-tracking software (EchoPAC PC, Version 110.0.2; GE Medical Systems, Horten, Norway) to an established semi-automated reference software (Artery Measurement System (AMS) II, Version 1.141; Gothenburg, Sweden) in 30 healthy preschool children (ages 3-5 years) and 27 adults with coronary artery disease (CAD; ages 48-81 years). For both groups, Bland-Altman plots revealed good agreement with a negligible mean cIMT difference of -0·03 mm. Software differences were statistically, but not clinically, significant for preschool images (P = 0·001) and were not significant for CAD images (P = 0·09). Intra- and interoperator repeatability was high and comparable between software for preschool images (ICC, 0·90-0·96; CV, 1·3-2·5%), but slightly higher with the automated ultrasound than the semi-automated reference software for CAD images (ICC, 0·98-0·99; CV, 1·4-2·0% versus ICC, 0·84-0·89; CV, 5·6-6·8%). These findings suggest that the automated ultrasound software produces valid cIMT values in healthy preschool children and adults with CAD. Automated ultrasound software may be useful for ensuring consistency among multisite research initiatives or large cohort studies involving repeated cIMT measures, particularly in adults with documented CAD. © 2017 Scandinavian Society of Clinical Physiology and Nuclear Medicine. Published by John Wiley & Sons Ltd.

  3. Previous radiotherapy and the clinical activity and toxicity of pembrolizumab in the treatment of non-small-cell lung cancer: a secondary analysis of the KEYNOTE-001 phase 1 trial.

    Science.gov (United States)

    Shaverdian, Narek; Lisberg, Aaron E; Bornazyan, Krikor; Veruttipong, Darlene; Goldman, Jonathan W; Formenti, Silvia C; Garon, Edward B; Lee, Percy

    2017-07-01

    Preclinical studies have found radiotherapy enhances antitumour immune responses. We aimed to assess disease control and pulmonary toxicity in patients who previously received radiotherapy for non-small-cell lung cancer (NSCLC) before receiving pembrolizumab. We assessed patients with advanced NSCLC treated on the phase 1 KEYNOTE-001 trial at a single institution (University of California, Los Angeles, CA, USA). Patients were aged 18 years or older, had an Eastern Cooperative Oncology Group performance status of 1 or less, had adequate organ function, and no history of pneumonitis. Patients received pembrolizumab at a dose of either 2 mg/kg of bodyweight or 10 mg/kg every 3 weeks, or 10 mg/kg every 2 weeks, until disease progression, unacceptable toxicity, or other protocol-defined reasons for discontinuation. Disease response and pulmonary toxicity were prospectively assessed by Immune-related Response Criteria and Common Terminology Criteria for Adverse Events version 4.0. The primary objective of the KEYNOTE-001 trial was to assess the safety, side-effect profile, and antitumour activity of pembrolizumab. For our secondary analysis, patients were divided into subgroups to compare patients who previously received radiotherapy with patients who had not. Our primary objective was to determine whether previous radiotherapy affected progression-free survival, overall survival, and pulmonary toxicity in the intention-to-treat population. The KEYNOTE-001 trial was registered with ClinicalTrials.gov, number NCT01295827. Between May 22, 2012, and July 11, 2014, 98 patients were enrolled and received their first cycle of pembrolizumab. One patient was lost to follow-up. 42 (43%) of 97 patients had previously received any radiotherapy for the treatment of NSCLC before the first cycle of pembrolizumab. 38 (39%) of 97 patients received extracranial radiotherapy and 24 (25%) of 97 patients received thoracic radiotherapy. Median follow-up for surviving patients was 32·5

  4. A Reference Viral Database (RVDB) To Enhance Bioinformatics Analysis of High-Throughput Sequencing for Novel Virus Detection.

    Science.gov (United States)

    Goodacre, Norman; Aljanahi, Aisha; Nandakumar, Subhiksha; Mikailov, Mike; Khan, Arifa S

    2018-01-01

    Detection of distantly related viruses by high-throughput sequencing (HTS) is bioinformatically challenging because of the lack of a public database containing all viral sequences, without abundant nonviral sequences, which can extend runtime and obscure viral hits. Our reference viral database (RVDB) includes all viral, virus-related, and virus-like nucleotide sequences (excluding bacterial viruses), regardless of length, and with overall reduced cellular sequences. Semantic selection criteria (SEM-I) were used to select viral sequences from GenBank, resulting in a first-generation viral database (VDB). This database was manually and computationally reviewed, resulting in refined, semantic selection criteria (SEM-R), which were applied to a new download of updated GenBank sequences to create a second-generation VDB. Viral entries in the latter were clustered at 98% by CD-HIT-EST to reduce redundancy while retaining high viral sequence diversity. The viral identity of the clustered representative sequences (creps) was confirmed by BLAST searches in NCBI databases and HMMER searches in PFAM and DFAM databases. The resulting RVDB contained a broad representation of viral families, sequence diversity, and a reduced cellular content; it includes full-length and partial sequences and endogenous nonretroviral elements, endogenous retroviruses, and retrotransposons. Testing of RVDBv10.2, with an in-house HTS transcriptomic data set indicated a significantly faster run for virus detection than interrogating the entirety of the NCBI nonredundant nucleotide database, which contains all viral sequences but also nonviral sequences. RVDB is publically available for facilitating HTS analysis, particularly for novel virus detection. It is meant to be updated on a regular basis to include new viral sequences added to GenBank. IMPORTANCE To facilitate bioinformatics analysis of high-throughput sequencing (HTS) data for the detection of both known and novel viruses, we have

  5. Evaluation of two public genome references for chinese hamster ovary cells in the context of rna-seq based gene expression analysis.

    Science.gov (United States)

    Chen, Chun; Le, Huong; Goudar, Chetan T

    2017-07-01

    RNA-Seq is a powerful transcriptomics tool for mammalian cell culture process development. Successful RNA-Seq data analysis requires a high quality reference for read mapping and gene expression quantification. Currently, there are two public genome references for Chinese hamster ovary (CHO) cells, the predominant mammalian cell line in the biopharmaceutical industry. In this study, we compared these two references by analyzing 60 RNA-Seq samples from a variety of CHO cell culture conditions. Among the 20,891 common genes in both references, we observed that 31.5% have more than 7.1% quantification differences, implying gene definition differences in the two references. We propose a framework to quantify this difference using two metrics, Consistency and Stringency, which account for the average quantification difference between the two references over all samples, and the sample-specific effect on the quantification result, respectively. These two metrics can be used to identify potential genes for future gene model improvement and to understand the reliability of differentially expressed genes identified by RNA-Seq data analysis. Before a more comprehensive genome reference for CHO cells emerges, the strategy proposed in this study can enable more robust transcriptome analysis from CHO cell RNA-Seq data. Biotechnol. Bioeng. 2017;114: 1603-1613. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  6. High-frequency QRS analysis compared to conventional ST-segment analysis in patients with chest pain and normal ECG referred for exercise tolerance test.

    Science.gov (United States)

    Conti, Alberto; Alesi, Andrea; Aspesi, Giovanna; De Bernardis, Niccolò; Bianchi, Simone; Coppa, Alessandro; Donnini, Chiara; Grifoni, Caterina; Becucci, Alessandro; Casula, Claudia

    2015-01-01

    The novel analysis of high-frequency QRS components (HFQRS-analysis) has been proposed in patients with chest pain (CP) and normal electrocardiography (ECG) referred for exercise tolerance test (ex-ECG). The aim of the study was to compare the diagnostic value of ex-ECG with ex-HFQRS-analysis. Patients with CP and normal ECG, troponin, and echocardiography were considered. All patients underwent ex-ECG for conventional ST-segment-analysis and ex-HFQRS-analysis. A decrease ≥ 50% of the HFQRS signal intensity recorded in at least 2 contiguous leads was considered an index of ischemia, as ST-segment depression ≥ 2 mm or ≥ 1 mm and CP on ex-ECG. Exclusion criteria were: QRS duration ≥ 120 ms and inability to exercise. End-point: The composite of coronary stenosis ≥ 70% or acute coronary syndrome, revascularization, cardiovascular death at 3-month follow-up. Three-hundred thirty-seven patients were enrolled (age 60 ± 15 years). The percent-age of age-adjusted maximal predicted heart rate was 89 ± 10 beat per minute and the maximal systolic blood pressure was 169 ± 23 mm Hg. Nineteen patients achieved the end-point. In multivariate analysis, both ex-ECG and ex-HFQRS were predictors of the end-point. The ex-HFQRS-analysis showed higher sensitivity (63% vs. 26%; p ECG-analysis. Receiver operator characteristics analysis showed the incremental diagnostic value of HFQRS (area: 0.655, 95% CI 0.60-0.71) over conventional ex-ECG (0.608, CI 0.55-0.66) and CP score (0.530, CI 0.48-0.59), however without statistical significance in pairwise comparison by C-statistic. In patients with CP submitted to ex-ECG, the novel ex-HFQRS-analysis shows a valuable incremental diagnostic value over ST-segment-analysis.

  7. Benefits of statistical molecular design, covariance analysis, and reference models in QSAR: a case study on acetylcholinesterase

    Science.gov (United States)

    Andersson, C. David; Hillgren, J. Mikael; Lindgren, Cecilia; Qian, Weixing; Akfur, Christine; Berg, Lotta; Ekström, Fredrik; Linusson, Anna

    2015-03-01

    Scientific disciplines such as medicinal- and environmental chemistry, pharmacology, and toxicology deal with the questions related to the effects small organic compounds exhort on biological targets and the compounds' physicochemical properties responsible for these effects. A common strategy in this endeavor is to establish structure-activity relationships (SARs). The aim of this work was to illustrate benefits of performing a statistical molecular design (SMD) and proper statistical analysis of the molecules' properties before SAR and quantitative structure-activity relationship (QSAR) analysis. Our SMD followed by synthesis yielded a set of inhibitors of the enzyme acetylcholinesterase (AChE) that had very few inherent dependencies between the substructures in the molecules. If such dependencies exist, they cause severe errors in SAR interpretation and predictions by QSAR-models, and leave a set of molecules less suitable for future decision-making. In our study, SAR- and QSAR models could show which molecular sub-structures and physicochemical features that were advantageous for the AChE inhibition. Finally, the QSAR model was used for the prediction of the inhibition of AChE by an external prediction set of molecules. The accuracy of these predictions was asserted by statistical significance tests and by comparisons to simple but relevant reference models.

  8. Antimony speciation analysis in sediment reference materials using high-performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry

    International Nuclear Information System (INIS)

    Potin-Gautier, M.; Pannier, F.; Quiroz, W.; Pinochet, H.; Gregori, I. de

    2005-01-01

    This work presents the development of suitable methodologies for determination of the speciation of antimony in sediment reference samples. Liquid chromatography with a post-column photo-oxidation step and hydride generation atomic fluorescence spectrometry as detection system is applied to the separation and determination of Sb(III), Sb(V) and trimethylantimony species. Post-column decomposition and hydride generation steps were studied for sensitive detection with the AFS detector. This method was applied to investigate the conditions under which speciation analysis of antimony in sediment samples can be carried out. Stability studies of Sb species during the extraction processes of solid matrices, using different reagents solutions, were performed. Results demonstrate that for the extraction yield and the stability of Sb species in different marine sediment extracts, citric acid in ascorbic acid medium was the best extracting solution for antimony speciation analysis in this matrix (between 55% and 65% of total Sb was recovered from CRMs, Sb(III) being the predominant species). The developed method allows the separation of the three compounds within 6 min with detection limits of 30 ng g -1 for Sb(III) and TMSbCl2 and 40 ng g -1 for Sb(V) in sediment samples

  9. Antimony speciation analysis in sediment reference materials using high-performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry

    Energy Technology Data Exchange (ETDEWEB)

    Potin-Gautier, M. [Laboratoire de Chimie Analytique, BioInorganique et Environnement LCABIE (UMR CNRS 3054), Universite de Pau et des pays de l' Adour, 64000 Pau (France); Pannier, F. [Laboratoire de Chimie Analytique, BioInorganique et Environnement LCABIE (UMR CNRS 3054), Universite de Pau et des pays de l' Adour, 64000 Pau (France)]. E-mail: Florence.pannier@univ-pau.fr; Quiroz, W. [Laboratoire de Chimie Analytique, BioInorganique et Environnement LCABIE (UMR CNRS 3054), Universite de Pau et des pays de l' Adour, 64000 Pau (France); Laboratorio de Quimica Analitica y Ambiental, Instituto de Quimica, Pontificia Universidad catolica de Valparaiso (Chile); Pinochet, H. [Laboratorio de Quimica Analitica y Ambiental, Instituto de Quimica, Pontificia Universidad catolica de Valparaiso (Chile); Gregori, I. de [Laboratorio de Quimica Analitica y Ambiental, Instituto de Quimica, Pontificia Universidad catolica de Valparaiso (Chile)

    2005-11-30

    This work presents the development of suitable methodologies for determination of the speciation of antimony in sediment reference samples. Liquid chromatography with a post-column photo-oxidation step and hydride generation atomic fluorescence spectrometry as detection system is applied to the separation and determination of Sb(III), Sb(V) and trimethylantimony species. Post-column decomposition and hydride generation steps were studied for sensitive detection with the AFS detector. This method was applied to investigate the conditions under which speciation analysis of antimony in sediment samples can be carried out. Stability studies of Sb species during the extraction processes of solid matrices, using different reagents solutions, were performed. Results demonstrate that for the extraction yield and the stability of Sb species in different marine sediment extracts, citric acid in ascorbic acid medium was the best extracting solution for antimony speciation analysis in this matrix (between 55% and 65% of total Sb was recovered from CRMs, Sb(III) being the predominant species). The developed method allows the separation of the three compounds within 6 min with detection limits of 30 ng g{sup -1} for Sb(III) and TMSbCl2 and 40 ng g{sup -1} for Sb(V) in sediment samples.

  10. Calibration-free quantitative elemental analysis of meteor plasma using reference laser-induced breakdown spectroscopy of meteorite samples

    Science.gov (United States)

    Ferus, Martin; Koukal, Jakub; Lenža, Libor; Srba, Jiří; Kubelík, Petr; Laitl, Vojtěch; Zanozina, Ekaterina M.; Váňa, Pavel; Kaiserová, Tereza; Knížek, Antonín; Rimmer, Paul; Chatzitheodoridis, Elias; Civiš, Svatopluk

    2018-03-01

    Aims: We aim to analyse real-time Perseid and Leonid meteor spectra using a novel calibration-free (CF) method, which is usually applied in the laboratory for laser-induced breakdown spectroscopic (LIBS) chemical analysis. Methods: Reference laser ablation spectra of specimens of chondritic meteorites were measured in situ simultaneously with a high-resolution laboratory echelle spectrograph and a spectral camera for meteor observation. Laboratory data were subsequently evaluated via the CF method and compared with real meteor emission spectra. Additionally, spectral features related to airglow plasma were compared with the spectra of laser-induced breakdown and electric discharge in the air. Results: We show that this method can be applied in the evaluation of meteor spectral data observed in real time. Specifically, CF analysis can be used to determine the chemical composition of meteor plasma, which, in the case of the Perseid and Leonid meteors analysed in this study, corresponds to that of the C-group of chondrites.

  11. Selection of reliable reference genes for gene expression analysis under abiotic stresses in the desert biomass willow, Salix psammophila

    Directory of Open Access Journals (Sweden)

    Jianbo Li

    2016-10-01

    Full Text Available Salix psammophila is a desert shrub willow that has extraordinary adaptation to abiotic stresses and plays an important role in maintaining local ecosystems. Moreover, S. psammophila is regarded as a promising biomass feedstock because of its high biomass yields and short rotation coppice cycle. However, few suitable reference genes (RGs for quantitative real-time polymerase chain reaction (qRT-PCR constrain the study on normalization of gene expression in S. psammophila until now. Here, we investigated the expression stabilities of 14 candidate RGs across tissue types and under four abiotic stress treatments, including heat, cold, salt and drought treatments. After calculation of PCR efficiencies, three different software, NormFinder, geNorm, and BestKeeper were employed to analyze systematically the qRT-PCR data, and the outputs were merged by RankAggreg software. The optimal reference genes selected for gene expression analysis were EF1□□ (Elongation factor-1 alpha and OTU (OTU-like cysteine protease family protein for different tissue types, UBC (Ubiquitin-conjugating enzyme E2 and LTA4H (Leukotriene A-4 hydrolase homologue for heat treatment, HIS (Histone superfamily protein H3 and ARF2 (ADP-ribosylation factor 2 for cold treatment, OTU and ACT7 (Actin 7 for salt treatment, UBC and LTA4H for drought treatment. The expression of UBC, ARF2 and VHAC (V-type proton ATPase subunit C varied the least across tissue types and under abiotic stresses. Furthermore, the relative genes expression profiles of one tissue-specific gene WOX1a (WUSCHEL-related homeobox 1a, and four stress-inducible genes, including Hsf-A2 (Heat shock transcription factors A2, CBF3 (C-repeat binding factor 3, HKT1 (High-Affinity K+ Transporter 1 and GST (Glutathione S-transferase, were conducted to confirm the validity of the reference genes in this study. These results provided an important RGs application guideline for gene expression characterization in S. psammophila.

  12. Analysis, Design, and Experimental Verification of A Synchronous Reference Frame Voltage Control for Single-Phase Inverters

    DEFF Research Database (Denmark)

    Monfared, Mohammad; Golestan, Saeed; Guerrero, Josep M.

    2014-01-01

    Control of three-phase power converters in the synchronous reference frame is now a mature and well developed research topic. However, for single-phase converters, it is not as well-established as three-phase applications. This paper deals with the design of a synchronous reference frame multi......-loop control strategy for single phase inverter-based islanded distributed generation (DG) systems. The proposed controller uses a synchronous reference frame PI (SRFPI) controller to regulate the instantaneous output voltage, a capacitor current shaping loop in the stationary reference frame to provide active...... in the synchronous reference frame, it is not straightforward to fine-tune the control parameters and evaluate the stability of the whole closed loop system. To overcome this problem, the stationary reference frame equivalent of the voltage loop is derived. Then, a step-by-step systematic design procedure based...

  13. Reference values for the geometric centre analysis of colonic transit measurements with 111indium-labelled diethylenetriamine penta-acetic acid

    DEFF Research Database (Denmark)

    Madsen, Jan Lysgård; Fuglsang, Stefan; Graff, Jesper

    2003-01-01

    The geometric centre analysis is often used for evaluating colonic transit data obtained by scintigraphy after oral intake of 111indium-labelled diethylenetriamine penta-acetic acid (111In-DTPA). The purpose of this study was to establish reference values for the geometric centre analysis in adult...

  14. Neutron activation analysis and ICP-AES to determine metal traces in antarctic krill. CNEA laboratories participation in the certification of a reference material

    International Nuclear Information System (INIS)

    Smichowski, Patricia N.; Farias, Silvia S.; Resnizky, Sara M.; Marrero, Julieta G.

    1999-01-01

    For the international certification of a reference material based on krill, As and Hg were determined by instrumental neutron activation analysis and Br, Co and Se by radiochemical neutron activation analysis. Inductive coupling plasma atomic emission spectrometry (ICP-AES) was used to determine Cu, Fe, Mn, and Zn. The results, which are in good agreement with those obtained by other laboratories, are discussed

  15. X-Ray Fluorescence On-Stream Analysis of Standard Reference Solution Concentrations of Chromium Plating and Polishing Solutions

    National Research Council Canada - National Science Library

    Sopok, Samuel

    1991-01-01

    X-ray fluorescence is evaluated as a means to quantitatively analyze standard reference solution concentrations of chromium plating and polishing solutions for chromium, sulfur, phosphorus, and iron...

  16. Lead isotopic compositions of environmental certified reference materials for an inter-laboratory comparison of lead isotope analysis

    International Nuclear Information System (INIS)

    Aung, Nyein Nyein; Uryu, Tsutomu; Yoshinaga, Jun

    2004-01-01

    Lead isotope ratios, viz. 207 Pb/ 206 Pb and 208 Pb/ 206 Pb, of the commercially available certified reference materials (CRMs) issued in Japan are presented with an objective to provide a data set, which will be useful for the quality assurance of analytical procedures, instrumental performance and method validation of the laboratories involved in environmental lead isotope ratio analysis. The analytical method used in the present study was inductively coupled plasma quadrupole mass spectrometry (ICPQMS) presented by acid digestion and with/without chemical separation of lead from the matrix. The precision of the measurements in terms of the relative standard deviation (RSD) of triplicated analyses was 0.19% and 0.14%, for 207 Pb/ 206 Pb and 208 Pb/ 206 Pb, respectively. The trueness of lead isotope ratio measurements of the present study was tested with a few CRMs, which have been analyzed by other analytical methods and reported in various literature. The lead isotopic ratios of 18 environmental matrix CRMs (including 6 CRMs analyzed for our method validation) are presented and the distribution of their ratios is briefly discussed. (author)

  17. Background qualitative analysis of the European reference life cycle database (ELCD) energy datasets - part II: electricity datasets.

    Science.gov (United States)

    Garraín, Daniel; Fazio, Simone; de la Rúa, Cristina; Recchioni, Marco; Lechón, Yolanda; Mathieux, Fabrice

    2015-01-01

    The aim of this paper is to identify areas of potential improvement of the European Reference Life Cycle Database (ELCD) electricity datasets. The revision is based on the data quality indicators described by the International Life Cycle Data system (ILCD) Handbook, applied on sectorial basis. These indicators evaluate the technological, geographical and time-related representativeness of the dataset and the appropriateness in terms of completeness, precision and methodology. Results show that ELCD electricity datasets have a very good quality in general terms, nevertheless some findings and recommendations in order to improve the quality of Life-Cycle Inventories have been derived. Moreover, these results ensure the quality of the electricity-related datasets to any LCA practitioner, and provide insights related to the limitations and assumptions underlying in the datasets modelling. Giving this information, the LCA practitioner will be able to decide whether the use of the ELCD electricity datasets is appropriate based on the goal and scope of the analysis to be conducted. The methodological approach would be also useful for dataset developers and reviewers, in order to improve the overall Data Quality Requirements of databases.

  18. Efficient Integration of Old and New Research Tools for Automating the Identification and Analysis of Seismic Reference Events

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, Robert; Rivers, Wilmer

    2005-01-25

    any single computer program for seismic data analysis will not have all the capabilities needed to study reference events, since hese detailed studies will be highly specialized. It may be necessary to develop and test new algorithms, and then these special ;odes must be integrated with existing software to use their conventional data-processing routines. We have investigated two neans of establishing communications between the legacy and new codes: CORBA and XML/SOAP Web services. We have nvestigated making new Java code communicate with a legacy C-language program, geotool, running under Linux. Both methods vere successful, but both were difficult to implement. C programs on UNIX/Linux are poorly supported for Web services, compared vith the Java and .NET languages and platforms. Easier-to-use middleware will be required for scientists to construct distributed applications as easily as stand-alone ones. Considerable difficulty was encountered in modifying geotool, and this problem shows he need to use component-based user interfaces instead of large C-language codes where changes to one part of the program nay introduce side effects into other parts. We have nevertheless made bug fixes and enhancements to that legacy program, but t remains difficult to expand it through communications with external software.

  19. Gastrointestinal stromal tumor: analysis of 146 cases of the center of reference of the National Cancer Institute--INCA.

    Science.gov (United States)

    Linhares, Eduardo; Gonçalves, Rinaldo; Valadão, Marcus; Vilhena, Bruno; Herchenhorn, Daniel; Romano, Sergio; Ferreira, Maria Aparecida; Ferreira, Carlos Gil; Ramos, Cintia de Araujo; de Jesus, José Paulo

    2011-01-01

    To evaluate the treatment of GIST in INCA. We conducted a retrospective analysis of all cases of GIST treated at INCA in the period from 1997 to 2009. We analyzed 146 patients with a mean age of 44.5 years and female predominance. The main symptom was abdominal pain. We observed the occurrence of a second primary tumor in 22% of cases and 92% of the immunohistochemistry exams were positive for CD117. The most frequent location was in the stomach and the high-risk group was predominant. Surgery was considered R0 (extensive) in 70% of the cases and the main sites of metastases were liver and peritoneum. Overall survival in two and five years was, respectively, 86% and 59%. There was a significant difference between overall survival (p = 0.29) of the high-risk group versus the other. Our patients presented mainly in the form of high-risk disease, with obvious impact on survival. The use of imatinib improved survival of patients with recurrent and metastatic disease. We should study its use in the setting of adjuvant and neoadjuvant therapy to improve results of the high risk group. The creation of reference centers is a need for the study of rare diseases.

  20. Determination of hexavalent chromium in plastic certified reference materials by X-ray absorption fine structure analysis

    International Nuclear Information System (INIS)

    Ohata, Masaki; Matsubayashi, Nobuyuki

    2014-01-01

    X-ray absorption fine structure (XAFS) analysis with transmission mode was used to determine the percentages of hexavalent chromium {Cr(VI)} in total Cr in plastic certified reference materials (CRMs). Cr-K edge X-ray absorption near-edge structure (XANES) spectra were observed and the normalized pre-edge peaks of the spectrum where absorption data was summed was acquired for the determination of Cr(VI). Examination of different number of data point and range of photon energy for summed absorption of the pre-edge peak resulted in reproducible absorption data, though the measurements were carried out at different beam time and beam line. The concentrations of Cr(VI) in the plastic CRMs were also estimated from both the certified value of total Cr and the determined percentage of Cr(VI). The analytical procedure and the estimated concentrations can be useful for the determination of Cr(VI) in plastics with respect to RoHS (restriction of the use of hazardous substances in electrical and electronics equipment) directive

  1. Satisfaction with Life Scale (SWLS) in caregivers of clinically-referred youth: psychometric properties and mediation analysis.

    Science.gov (United States)

    Athay, M Michele

    2012-03-01

    This paper presents the psychometric evaluation of the Satisfaction with Life Scale (SWLS; Diener et al. in J Personal Assess 49:71-75, 1985) used with a large sample (N = 610) of caregivers for youth receiving mental health services. Methods from classical test theory, factor analysis, and item response theory were utilized. Additionally, this paper investigated whether caregiver strain mediates the effect of youth symptom severity on caregiver life satisfaction (N = 356). Bootstrapped confidence intervals were used to determine the significance of the mediated effects. Results indicated that the SWLS is a psychometrically sound instrument to be used with caregivers of clinically-referred youth. Mediation analyses found that the effect of youth symptom severity on caregiver life satisfaction was mediated by caregiver strain but that the mediation effect differed based on the type of youth symptoms: caregiver strain was a partial mediator when externalizing symptoms were measured and a full mediator when internalizing symptoms were measured. Implications for future research and clinical practice are discussed.

  2. Selection and evaluation of reference genes for analysis of mouse (Mus musculus sex-dimorphic brain development

    Directory of Open Access Journals (Sweden)

    Tanya T. Cheung

    2017-01-01

    Full Text Available The development of the brain is sex-dimorphic, and as a result so are many neurological disorders. One approach for studying sex-dimorphic brain development is to measure gene expression in biological samples using RT-qPCR. However, the accuracy and consistency of this technique relies on the reference gene(s selected. We analyzed the expression of ten reference genes in male and female samples over three stages of brain development, using popular algorithms NormFinder, GeNorm and Bestkeeper. The top ranked reference genes at each time point were further used to quantify gene expression of three sex-dimorphic genes (Wnt10b, Xist and CYP7B1. When comparing gene expression between the sexes expression at specific time points the best reference gene combinations are: Sdha/Pgk1 at E11.5, RpL38/Sdha E12.5, and Actb/RpL37 at E15.5. When studying expression across time, the ideal reference gene(s differs with sex. For XY samples a combination of Actb/Sdha. In contrast, when studying gene expression across developmental stage with XX samples, Sdha/Gapdh were the top reference genes. Our results identify the best combination of two reference genes when studying male and female brain development, and emphasize the importance of selecting the correct reference genes for comparisons between developmental stages.

  3. Laying a Foundation for Comparing Departmental Structures between Reference and Instructional Services: Analysis of a Nationwide Survey.

    Science.gov (United States)

    Johnson, Kristin; Fountain, Kathleen Carlisle

    2002-01-01

    Describes a survey of midsized academic libraries that measured the departmental relationship between librarians who provide reference services and librarians who provide instructional services. Discusses satisfaction levels with various organizational arrangements as well as pros and cons of integrated or separate reference and instruction…

  4. VAGINAL PROGESTERONE VERSUS CERVICAL CERCLAGE FOR THE PREVENTION OF PRETERM BIRTH IN WOMEN WITH A SONOGRAPHIC SHORT CERVIX, SINGLETON GESTATION, AND PREVIOUS PRETERM BIRTH: A SYSTEMATIC REVIEW AND INDIRECT COMPARISON META-ANALYSIS

    Science.gov (United States)

    CONDE-AGUDELO, Agustin; ROMERO, Roberto; NICOLAIDES, Kypros; CHAIWORAPONGSA, Tinnakorn; O'BRIEN, John M.; CETINGOZ, Elcin; DA FONSECA, Eduardo; CREASY, George; SOMA-PILLAY, Priya; FUSEY, Shalini; CAM, Cetin; ALFIREVIC, Zarko; HASSAN, Sonia S.

    2012-01-01

    OBJECTIVE No randomized controlled trial has directly compared vaginal progesterone and cervical cerclage for the prevention of preterm birth in women with a sonographic short cervix in the midtrimester, singleton gestation, and previous spontaneous preterm birth. We performed an indirect comparison of vaginal progesterone versus cerclage, using placebo/no cerclage as the common comparator. STUDY DESIGN Adjusted indirect meta-analysis of randomized controlled trials. RESULTS Four studies evaluating vaginal progesterone versus placebo (158 patients) and five evaluating cerclage versus no cerclage (504 patients) were included. Both interventions were associated with a statistically significant reduction in the risk of preterm birth <32 weeks of gestation and composite perinatal morbidity and mortality compared with placebo/no cerclage. Adjusted indirect meta-analyses did not show statistically significant differences between vaginal progesterone and cerclage in reducing preterm birth or adverse perinatal outcomes. CONCLUSION Based on state-of-the-art methodology for indirect comparisons, either vaginal progesterone or cerclage are equally efficacious in the prevention of preterm birth in women with a sonographic short cervix in the midtrimester, singleton gestation, and previous preterm birth. The selection of the optimal treatment may depend upon adverse events, cost and patient/clinician preferences. PMID:23157855

  5. International Geomagnetic Reference Field

    DEFF Research Database (Denmark)

    Finlay, Chris; Maus, S.; Beggan, C. D.

    2010-01-01

    The eleventh generation of the International Geomagnetic Reference Field (IGRF) was adopted in December 2009 by the International Association of Geomagnetism and Aeronomy Working Group V‐MOD. It updates the previous IGRF generation with a definitive main field model for epoch 2005.0, a main field...

  6. An analysis of references used for the Orthopaedic In-Training Examination: what are their levels of evidence and journal impact factors?

    Science.gov (United States)

    Haughom, Bryan D; Goldstein, Zach; Hellman, Michael D; Yi, Paul H; Frank, Rachel M; Levine, Brett R

    2014-12-01

    Although the references recommended for the Orthopaedic In-Training Examination (OITE) have been evaluated in certain subspecialty domains, suggested reference level of evidence (LOE), impact factor, and citation age have not been evaluated comprehensively to our knowledge. We present an analysis of all references cited in the OITE recommended readings for each test question including the duration of time between their initial publication and their use in the OITE, which we defined as citation age, LOE, and the impact factor of the journals referenced. We evaluated all references for the 2010 to 2012 OITE administrations (three examinations; 825 questions total). Publication characteristics, including citation age, were noted. The LOE for each journal article and the impact factor of each journal were determined; differences in LOE and impact factor were compared between test sections. A total of 1817 references were cited in the 825 questions we evaluated; this denominator was used in all calculations that follow. The recommended reading references included 1337 journal article references (74%), 469 text references (26%), and 11 multimedia sources (0.6%; eg, websites, instructional DVDs). The three most commonly recommended journals were general orthopaedic journals, The Journal of Bone and Joint Surgery (American Volume), Journal of American Academy of Orthopaedic Surgeons, and Clinical Orthopaedics and Related Research. The majority (72.2%) of the cited journal references were published within 10 years of the test date, with a mean ± SD citation age of 8.3 ± 7.4 years. The majority of the cited journal articles were Levels IV and V evidence (mean, 4.16 ± 1.1). The Spine section had higher LOE (3.74; p references, and may provide insight for educators designing resident educational curricula.

  7. Long-term effectiveness of bee venom acupuncture and physiotherapy in the treatment of adhesive capsulitis: a one-year follow-up analysis of a previous randomized controlled trial.

    Science.gov (United States)

    Park, Yeon Cheol; Koh, Pil Seong; Seo, Byung Kwan; Lee, Jung Woo; Cho, Nam Su; Park, Hyung Soon; Park, Dong Suk; Baek, Yong Hyeon

    2014-12-01

    An earlier randomized controlled trial (RCT) study showed that bee venom acupuncture (BVA) in combination with physiotherapy can be more effective in functional improvement and pain reduction in patients with adhesive capsulitis (AC). The objective of the current study was to examine the long-term effect of BVA in combination with physiotherapy on AC of the shoulder. Retrospective 1-year follow-up analysis of a previous RCT using a telephone interview method. Outpatient joint center at the Gang Dong Kyung Hee University Hospital of Seoul, Republic of Korea. A total of 192 patients had been enrolled in the previous study, and 124 of these were excluded from the current study. Sixty-eight patients who had been treated with combined BVA and physiotherapy for AC of the shoulder for 2 months were interviewed at approximately 1 year after treatment by telephone. Sixty of 68 patients were included in the follow-up analysis. Twenty received BV 1 treatment (1:10,000 concentration BVA plus physiotherapy), 22 received BV 2 treatment (1:30,000 concentration BVA plus physiotherapy), and 18 received control treatment (normal saline injection plus physiotherapy). The primary outcome measure was Shoulder Pain And Disability Index (SPADI) score. Secondary outcome measure was score on verbal rating scale for pain and patient satisfaction. Baseline characteristics of the groups did not significantly differ. SPADI scores at 1 year significantly differed between the BV 1 group and the control group (p=0.043). No significant differences were found in pain verbal rating scores after 1 year. Treatment satisfaction with therapy was also assessed, and the BV 1 and BV 2 groups showed significantly greater satisfaction compared with the control group. BVA combined with physiotherapy remains clinically effective 1 year after treatment and may help improve long-term quality of life in patients with AC of the shoulder.

  8. Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

    Directory of Open Access Journals (Sweden)

    Müller Marcel A

    2005-02-01

    Full Text Available Abstract Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.

  9. Identification of suitable reference genes for gene expression normalization in qRT-PCR analysis in watermelon.

    Directory of Open Access Journals (Sweden)

    Qiusheng Kong

    Full Text Available Watermelon is one of the major Cucurbitaceae crops and the recent availability of genome sequence greatly facilitates the fundamental researches on it. Quantitative real-time reverse transcriptase PCR (qRT-PCR is the preferred method for gene expression analyses, and using validated reference genes for normalization is crucial to ensure the accuracy of this method. However, a systematic validation of reference genes has not been conducted on watermelon. In this study, transcripts of 15 candidate reference genes were quantified in watermelon using qRT-PCR, and the stability of these genes was compared using geNorm and NormFinder. geNorm identified ClTUA and ClACT, ClEF1α and ClACT, and ClCAC and ClTUA as the best pairs of reference genes in watermelon organs and tissues under normal growth conditions, abiotic stress, and biotic stress, respectively. NormFinder identified ClYLS8, ClUBCP, and ClCAC as the best single reference genes under the above experimental conditions, respectively. ClYLS8 and ClPP2A were identified as the best reference genes across all samples. Two to nine reference genes were required for more reliable normalization depending on the experimental conditions. The widely used watermelon reference gene 18SrRNA was less stable than the other reference genes under the experimental conditions. Catalase family genes were identified in watermelon genome, and used to validate the reliability of the identified reference genes. ClCAT1and ClCAT2 were induced and upregulated in the first 24 h, whereas ClCAT3 was downregulated in the leaves under low temperature stress. However, the expression levels of these genes were significantly overestimated and misinterpreted when 18SrRNA was used as a reference gene. These results provide a good starting point for reference gene selection in qRT-PCR analyses involving watermelon.

  10. Validation of reference genes for gene expression analysis in olive (Olea europaea) mesocarp tissue by quantitative real-time RT-PCR.

    Science.gov (United States)

    Ray, Debashree L; Johnson, Joshua C

    2014-05-18

    Gene expression analysis using quantitative reverse transcription PCR (qRT-PCR) is a robust method wherein the expression levels of target genes are normalised using internal control genes, known as reference genes, to derive changes in gene expression levels. Although reference genes have recently been suggested for olive tissues, combined/independent analysis on different cultivars has not yet been tested. Therefore, an assessment of reference genes was required to validate the recent findings and select stably expressed genes across different olive cultivars. A total of eight candidate reference genes [glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine/threonine-protein phosphatase catalytic subunit (PP2A), elongation factor 1 alpha (EF1-alpha), polyubiquitin (OUB2), aquaporin tonoplast intrinsic protein (TIP2), tubulin alpha (TUBA), 60S ribosomal protein L18-3 (60S RBP L18-3) and polypyrimidine tract-binding protein homolog 3 (PTB)] were chosen based on their stability in olive tissues as well as in other plants. Expression stability was examined by qRT-PCR across 12 biological samples, representing mesocarp tissues at various developmental stages in three different olive cultivars, Barnea, Frantoio and Picual, independently and together during the 2009 season with two software programs, GeNorm and BestKeeper. Both software packages identified GAPDH, EF1-alpha and PP2A as the three most stable reference genes across the three cultivars and in the cultivar, Barnea. GAPDH, EF1-alpha and 60S RBP L18-3 were found to be most stable reference genes in the cultivar Frantoio while 60S RBP L18-3, OUB2 and PP2A were found to be most stable reference genes in the cultivar Picual. The analyses of expression stability of reference genes using qRT-PCR revealed that GAPDH, EF1-alpha, PP2A, 60S RBP L18-3 and OUB2 are suitable reference genes for expression analysis in developing Olea europaea mesocarp tissues, displaying the highest level of expression stability across

  11. Roaming Reference: Reinvigorating Reference through Point of Need Service

    Directory of Open Access Journals (Sweden)

    Kealin M. McCabe

    2011-11-01

    Full Text Available Roaming reference service was pursued as a way to address declining reference statistics. The service was staffed by librarians armed with iPads over a period of six months during the 2010-2011 academic year. Transactional statistics were collected in relation to query type (Research, Facilitative or Technology, location and approach (librarian to patron, patron to librarian or via chat widget. Overall, roaming reference resulted in an additional 228 reference questions, 67% (n=153 of which were research related. Two iterations of the service were implemented, roaming reference as a standalone service (Fall 2010 and roaming reference integrated with traditional reference desk duties (Winter 2011. The results demonstrate that although the Weller Library’s reference transactions are declining annually, they are not disappearing. For a roaming reference service to succeed, it must be a standalone service provided in addition to traditional reference services. The integration of the two reference models (roaming reference and reference desk resulted in a 56% decline in the total number of roaming reference questions from the previous term. The simple act of roaming has the potential to reinvigorate reference services as a whole, forcing librarians outside their comfort zones, allowing them to reach patrons at their point of need.

  12. Certification of methylmercury in cod fish tissue certified reference material by species-specific isotope dilution mass spectrometric analysis

    Energy Technology Data Exchange (ETDEWEB)

    Inagaki, Kazumi; Kuroiwa, Takayoshi; Narukawa, Tomohiro; Yarita, Takashi; Takatsu, Akiko; Okamoto, Kensaku; Chiba, Koichi [National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Environmental Standard Section, Tsukuba, Ibaraki (Japan)

    2008-07-15

    A new cod fish tissue certified reference material, NMIJ CRM 7402-a, for methylmercury analysis was certified by the National Metrological Institute of Japan in the National Institute of Advanced Industrial Science and Technology (NMIJ/AIST). Cod fish was collected from the sea close to Japan. The cod muscle was powdered by freeze-pulverization and was placed into 600 glass bottles (10 g each), which were sterilized with {gamma}-ray irradiation. The certification was carried out using species-specific isotope dilution gas chromatography inductively coupled plasma mass spectrometry (SSID-GC-ICPMS), where {sup 202}Hg-enriched methylmercury (MeHg) was used as the spike compound. In order to avoid any possible analytical biases caused by nonquantitative extraction, degradation and/or formation of MeHg in sample preparations, two different extraction methods (KOH/methanol and HCl/methanol extractions) were performed, and one of these extraction methods utilized two different derivatization methods (ethylation and phenylation). A double ID method was adopted to minimize the uncertainty arising from the analyses. In order to ensure not only the reliability of the analytical results but also traceability to SI units, the standard solution of MeHg used for the reverse-ID was prepared from high-purity MeHg chloride and was carefully assayed as follows: the total mercury was determined by ID-ICPMS following aqua regia digestion, and the ratio of Hg as MeHg to the total Hg content was estimated by GC-ICPMS. The certified value given for MeHg is 0.58 {+-} 0.02 mg kg{sup -1} as Hg. (orig.)

  13. Diagnostic Accuracy of Transthoracic Echocardiography for Infective Endocarditis Findings Using Transesophageal Echocardiography as the Reference Standard: A Meta-Analysis.

    Science.gov (United States)

    Bai, Anthony D; Steinberg, Marilyn; Showler, Adrienne; Burry, Lisa; Bhatia, R Sacha; Tomlinson, George A; Bell, Chaim M; Morris, Andrew M

    2017-07-01

    Echocardiography is important for the diagnosis of infective endocarditis (IE), for which transesophageal echocardiography (TEE) is superior to transthoracic echocardiography (TTE). A systematic review and meta-analysis of observational studies was performed with the objective of evaluating diagnostic properties of TTE, with transesophageal findings of IE as the reference standard in patients with suspected IE. The literature search yielded 377 unique articles, of which 16 met the inclusion criteria. The 16 studies included 2,807 patients, of whom 793 (28%) had vegetations on TEE. For detecting vegetations, harmonic TTE had sensitivity of 61% (95% CI, 45%-75%) and specificity of 94% (95% CI, 85%-98%) with a negative likelihood ratio (NLR) of 0.42 (95% CI, 0.26-0.61). NLR for harmonic TTE can be improved by including only patients without prosthetic valves (NLR = 0.36; 95% CI, 0.22-0.55) or by having strict criteria for conclusively negative results on TTE (NLR = 0.17; 95% CI, 0.10-0.28). In the setting of patients without prosthetic valves, harmonic TTE had likelihood ratios of 0.14 (95% CI, 0.09-0.23) for a conclusively negative result, 0.66 (95% CI, 0.53-0.81) for an indeterminate result, and 14.60 (95% CI, 3.37-70.40) for a positive result. Modern harmonic TTE still has the potential to miss many vegetations detected on TEE. When limited to patients without prosthetic valves, a conclusively negative TTE under optimal view greatly decreases likelihood of IE. All other transthoracic results are not useful for ruling out IE, and subsequent TEE is almost always required. Copyright © 2017 American Society of Echocardiography. Published by Elsevier Inc. All rights reserved.

  14. Use of reference materials for validating analytical methods. Applied to the determination of As, Co, Na, Hg, Se and Fe using neutron activation analysis

    International Nuclear Information System (INIS)

    Munoz, L; Andonie, O; Kohnenkamp, I

    2000-01-01

    The main purpose of an analytical laboratory is to provide reliable information on the nature and composition of the materials submitted for analysis. This purpose can only be attained if analytical methodologies that have the attributes of accuracy, precision, specificity and sensitivity, among others, are used. The process by which these attributes are evaluated is called validation of the analytical method. The Chilean Nuclear Energy Commission's Neutron Activation Analysis Laboratory is applying a quality guarantee program to ensure the quality of its analytical results, which aims, as well, to attain accreditation for some of its measurements. Validation of the analytical methodologies used is an essential part of applying this program. There are many forms of validation, from comparison with reference techniques to participation in inter-comparison rounds. Certified reference materials were used in this work in order to validate the application of neutron activation analysis in determining As, Co, Na, Hg, Se and Fe in shellfish samples. The use of reference materials was chosen because it is a simple option that easily detects sources of systematic errors. Neutron activation analysis is an instrumental analytical method that does not need chemical treatment and that is based on processes which take place in the nuclei of atoms, making the matrix effects unimportant and different biological reference materials can be used. The following certified reference materials were used for validating the method used: BCR human hair 397, NRCC dogfish muscle DORM-2, NRCC -dogfish liver DOLT-2, NIST - oyster tissue 1566, NIES - mussel 6 and BCR - tuna fish 464. The reference materials were analyzed using the procedure developed for the shellfish samples and the above-mentioned elements were determined. With the results obtained, the parameters of accuracy, precision, detection limit, quantification limit and uncertainty associated with the method were determined for each

  15. [Bibliometric study of the journal Nutrición Hospitalaria for the period 2001--2005: Part 2, consumption analysis; the bibliographic references].

    Science.gov (United States)

    Castera, V T; Sanz Valero, J; Juan-Quilis, V; Wanden-Berghe, C; Culebras, J M; García de Lorenzo y Mateos, A

    2008-01-01

    To describe and assess the consumption of the information consulted and cited in the articles published in the journal Nutrición Hospitalaria for the period 2001--2005 by means of bibliometric analysis. Cross-sectional descriptive analysis of the results obtained from the analysis of the lists of bibliographic references of the articles published at Nutrición Hospitalaria. We studied the most cited journals, the signatures index, the type of document referred, the publication language, the distribution of geographical origin, and obsolescence and readiness index. We took into account all types of documents with the exception of Communications to Congresses. 345 articles were published at Nutr Hosp, containing 8,113 bibliographic references, with a median of 18, a maximum of 136 and minimum of 0 BR per article. The mean (rate of publications per published article during the specified period) is 23.52 (95% IC 20.93-26.10) and the mean at 5% is 20.66 per article. The 25th and 75th percentiles are 6 and 32, respectively, the interquartile interval being 26 BR per document. The semi-period of Burton and Kebler is 7 years and the Price Index is 38.18%. The bibliographic references, the consumption of information, of the articles published at Nutrición Hospitalaria present parameters similar to other journals on health science. However, good data on obsolescence are observed, which reveal the good validity of most of the references studied.

  16. Commercial reference shape standards use in the study of particle shape effect on laser diffraction particle size analysis.

    Science.gov (United States)

    Kelly, Richard N; Kazanjian, Jacqueline

    2006-05-26

    The purpose of this paper is to describe the use of LGC Promochem AEA 1001 to AEA 1003 monosized fiber-analog shape standards in the study of the effect of particle shape on laser diffraction (LD) particle size analysis (psa). The psa of the AEA standards was conducted using LD psa systems from Beckman Coulter, Horiba, and Malvern Instruments. Flow speed settings, sample refractive index values, and sample cell types were varied to examine the extent to which the shape effect on LD psa results is modified by these variables. The volume and number probability plots resulting from these measurements were each characterized by a spread in the particle size distribution that roughly extended from the breadth to the longest dimension of the particles. For most of the selected sample refractive index values, the volume probability plots were characterized by apparent bimodal distributions. The results, therefore, provide experimental verification of the conclusions from theoretical studies of LD psa system response to monosized elliptical particles in which this apparent bimodality was the predicted result in the case of flow-oriented particles. The data support the findings from previous studies conducted over the past 10 years that have called into question the verity of the tenets of, and therefore the value of the application of, the equivalent spherical volume diameter theory and the random particle orientation model to the interpretation of LD psa results from measurements made on nonspherical particles.

  17. Analysis on anatomical references to assess the coronal alignment of tibial and femoral cuts in mega prosthetic knee replacement.

    Science.gov (United States)

    Karade, Vikas; Ravi, Bhallamudi

    2014-06-01

    In megaprosthetic knee replacement, surgeons use cutting guides that depend on anatomical references to determine the ideal cutting plane alignment. In this work, we investigated the accuracy of using femoral cortical surfaces and tibial canal portions as the references. The study aims to improve the design and use of the cutting guides. Sixty-one knee scanograms of 33 patients (mean age around 20 years) diagnosed with osteogenic sarcoma and undergoing distal femur megaprosthetic surgery were acquired. Angles between the selected anatomical references and axis perpendicular to the ideal cutting plane (anatomical axis for femur and mechanical axis for tibia) were measured for both femur and tibia, in coronal view. The smaller the magnitude of the angles, the better the anatomical reference is. At the central femoral region, on average, both lateral and medial cortical surfaces give accurate alignment of the ideal cutting plane (0.6° and 0.8°, respectively), with no significant difference (p > 0.01). At the distal region, the lateral cortical surface gives significantly better alignment compared to the medial cortical surface (p references, but only at the central region. For a tibial cut, the central anatomical axis is the best reference. IV.

  18. Reference gene selection for qRT-PCR analysis in the sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae).

    Science.gov (United States)

    Li, Rumei; Xie, Wen; Wang, Shaoli; Wu, Qingjun; Yang, Nina; Yang, Xin; Pan, Huipeng; Zhou, Xiaomao; Bai, Lianyang; Xu, Baoyun; Zhou, Xuguo; Zhang, Youjun

    2013-01-01

    Accurate evaluation of gene expression requires normalization relative to the expression of reliable reference genes. Expression levels of "classical" reference genes can differ, however, across experimental conditions. Although quantitative real-time PCR (qRT-PCR) has been used extensively to decipher gene function in the sweetpotato whitefly Bemisia tabaci, a world-wide pest in many agricultural systems, the stability of its reference genes has rarely been validated. In this study, 15 candidate reference genes from B. tabaci were evaluated using two Excel-based algorithms geNorm and Normfinder under a diverse set of biotic and abiotic conditions. At least two reference genes were selected to normalize gene expressions in B. tabaci under experimental conditions. Specifically, for biotic conditions including host plant, acquisition of a plant virus, developmental stage, tissue (body region of the adult), and whitefly biotype, ribosomal protein L29 was the most stable reference gene. In contrast, the expression of elongation factor 1 alpha, peptidylprolyl isomerase A, NADH dehydrogenase, succinate dehydrogenase complex subunit A and heat shock protein 40 were consistently stable across various abiotic conditions including photoperiod, temperature, and insecticide susceptibility. Our finding is the first step toward establishing a standardized quantitative real-time PCR procedure following the MIQE (Minimum Information for publication of Quantitative real time PCR Experiments) guideline in an agriculturally important insect pest, and provides a solid foundation for future RNA interference based functional study in B. tabaci.

  19. Reference gene selection for qRT-PCR analysis in the sweetpotato whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae.

    Directory of Open Access Journals (Sweden)

    Rumei Li

    Full Text Available Accurate evaluation of gene expression requires normalization relative to the expression of reliable reference genes. Expression levels of "classical" reference genes can differ, however, across experimental conditions. Although quantitative real-time PCR (qRT-PCR has been used extensively to decipher gene function in the sweetpotato whitefly Bemisia tabaci, a world-wide pest in many agricultural systems, the stability of its reference genes has rarely been validated.In this study, 15 candidate reference genes from B. tabaci were evaluated using two Excel-based algorithms geNorm and Normfinder under a diverse set of biotic and abiotic conditions. At least two reference genes were selected to normalize gene expressions in B. tabaci under experimental conditions. Specifically, for biotic conditions including host plant, acquisition of a plant virus, developmental stage, tissue (body region of the adult, and whitefly biotype, ribosomal protein L29 was the most stable reference gene. In contrast, the expression of elongation factor 1 alpha, peptidylprolyl isomerase A, NADH dehydrogenase, succinate dehydrogenase complex subunit A and heat shock protein 40 were consistently stable across various abiotic conditions including photoperiod, temperature, and insecticide susceptibility.Our finding is the first step toward establishing a standardized quantitative real-time PCR procedure following the MIQE (Minimum Information for publication of Quantitative real time PCR Experiments guideline in an agriculturally important insect pest, and provides a solid foundation for future RNA interference based functional study in B. tabaci.

  20. [Bibliometric study of the original articles published in Revista Española de Salud Púiblica (1991-2000). Part III: reference analysis].

    Science.gov (United States)

    Villar Alvarez, Fernando; Estrada Lorenzo, José Manuel; Peréz Andrés, Christina; Rebollo Rodríguez, M José

    2007-01-01

    The advancement of knowledge is based on the results of previously conducted research studies, which are reflected in the reference sources listed in a scientific article. This study is aimed at studying the scientific information used in the Revista Española de Salud Pública based on the references cited in the original articles published during the 1991-2000 period. The data regarding the year and where published, document type, language and country in which published was taken from the reference sources listed in the 290 original articles published, the obsolescence, Price and isolation indexes being calculated, and the Bradford core distribution being established according to the source journals. The self-citing rate was also calculated. A total of 7465 references were cited in the Reference section of the 290 original articles. An average of 25.7 references were cited per article. The Price index was 40.7. The scientific articles showed an obsolescence index of 5, the books and book chapters having an index of 6. A total 50.6% of the citations were from studies published in Spanish. The isolation index of the references was 48.1. The first Bradford core is comprised of 10 journals, the first four of which are Spanish. The self-citing rate was 3.8%. The information consumption of the original articles published in the Revista Española de Salud Pública show parameters similar to those of other Spanish health sciences journals for those same years, and the parameters regarding which this Journal differs from other Spanish health sciences journals seem to be justified by those particular aspects unique to public health, which does not fall within the patterns inherent to the clinical disciplines.

  1. Incidence of Acneform Lesions in Previously Chemically Damaged Persons-2004

    Directory of Open Access Journals (Sweden)

    N Dabiri

    2008-04-01

    Full Text Available ABSTRACT: Introduction & Objective: Chemical gas weapons especially nitrogen mustard which was used in Iraq-Iran war against Iranian troops have several harmful effects on skin. Some other chemical agents also can cause acne form lesions on skin. The purpose of this study was to compare the incidence of acneform in previously chemically damaged soldiers and non chemically damaged persons. Materials & Methods: In this descriptive and analytical study, 180 chemically damaged soldiers, who have been referred to dermatology clinic between 2000 – 2004, and forty non-chemically damaged people, were chosen randomly and examined for acneform lesions. SPSS software was used for statistic analysis of the data. Results: The mean age of the experimental group was 37.5 ± 5.2 and that of the control group was 38.7 ± 5.9 years. The mean percentage of chemical damage in cases was 31 percent and the time after the chemical damage was 15.2 ± 1.1 years. Ninety seven cases (53.9 percent of the subjects and 19 people (47.5 percent of the control group had some degree of acne. No significant correlation was found in incidence, degree of lesions, site of lesions and age of subjects between two groups. No significant correlation was noted between percentage of chemical damage and incidence and degree of lesions in case group. Conclusion: Incidence of acneform lesions among previously chemically injured peoples was not higher than the normal cases.

  2. CASKS (Computer Analysis of Storage casKS): A microcomputer based analysis system for storage cask design review. User's manual to Version 1b (including program reference)

    International Nuclear Information System (INIS)

    Chen, T.F.; Gerhard, M.A.; Trummer, D.J.; Johnson, G.L.; Mok, G.C.

    1995-02-01

    CASKS (Computer Analysis of Storage casKS) is a microcomputer-based system of computer programs and databases developed at the Lawrence Livermore National Laboratory (LLNL) for evaluating safety analysis reports on spent-fuel storage casks. The bulk of the complete program and this user's manual are based upon the SCANS (Shipping Cask ANalysis System) program previously developed at LLNL. A number of enhancements and improvements were added to the original SCANS program to meet requirements unique to storage casks. CASKS is an easy-to-use system that calculates global response of storage casks to impact loads, pressure loads and thermal conditions. This provides reviewers with a tool for an independent check on analyses submitted by licensees. CASKS is based on microcomputers compatible with the IBM-PC family of computers. The system is composed of a series of menus, input programs, cask analysis programs, and output display programs. All data is entered through fill-in-the-blank input screens that contain descriptive data requests

  3. Consuming America : A Data-Driven Analysis of the United States as a Reference Culture in Dutch Public Discourse on Consumer Goods, 1890-1990

    NARCIS (Netherlands)

    Wevers, M.J.H.F.

    2017-01-01

    Consuming America offers a data-driven, longitudinal analysis of the historical dynamics that have underpinned a long-term, layered cultural-historical process: the emergence of the United States as a dominant reference culture in Dutch public discourse on consumer goods between 1890 and 1990. The

  4. Evaluation of a new electronic preoperative reference marker for toric intraocular lens implantation by two different methods of analysis: Adobe Photoshop versus iTrace

    Directory of Open Access Journals (Sweden)

    Javed Hussain Farooqui

    2017-01-01

    CONCLUSIONS: Difference in reference mark positions when analyzed by two systems suggests the presence of varying cyclotorsion at different points of time. Both analysis methods showed an approximately 3° of alignment error, which could contribute to 10% loss of astigmatic correction of toric IOL. This can be further compounded by intra-operative marking errors and final placement of IOL in the bag.

  5. Stats Don't Tell the Whole Story: Using Qualitative Data Analysis of Chat Reference Transcripts to Assess and Improve Services

    Science.gov (United States)

    Mungin, Michael

    2017-01-01

    In the five years following implementation of a chat reference service at James Madison University (JMU), the service proved very popular but was not closely assessed for quality of service. Using grounded theory and qualitative data analysis techniques, a comprehensive assessment effort was begun in earnest and is in progress. Preliminary results…

  6. Extending reference assembly models

    DEFF Research Database (Denmark)

    Church, Deanna M.; Schneider, Valerie A.; Steinberg, Karyn Meltz

    2015-01-01

    The human genome reference assembly is crucial for aligning and analyzing sequence data, and for genome annotation, among other roles. However, the models and analysis assumptions that underlie the current assembly need revising to fully represent human sequence diversity. Improved analysis tools...... and updated data reporting formats are also required....

  7. Large genomic rearrangements of BRCA1 and BRCA2 among patients referred for genetic analysis in Galicia (NW Spain): delimitation and mechanism of three novel BRCA1 rearrangements.

    Science.gov (United States)

    Fachal, Laura; Blanco, Ana; Santamariña, Marta; Carracedo, Angel; Vega, Ana

    2014-01-01

    In the Iberian Peninsula, which includes mainly Spain and Portugal, large genomic rearrangements (LGRs) of BRCA1 and BRCA2 have respectively been found in up to 2.33% and 8.4% of families with hereditary breast and/or ovarian cancer (HBOC) that lack point mutations and small indels. In Galicia (Northwest Spain), the spectrum and frequency of BRCA1/BRCA2 point mutations differs from the rest of the Iberian populations. However, to date there are no Galician frequency reports of BRCA1/BRCA2 LGRs. Here we used multiplex ligation-dependent probe amplification (MLPA) to screen 651 Galician index cases (out of the 830 individuals referred for genetic analysis) without point mutations or small indels. We identified three different BRCA1 LGRs in four families. Two of them have been previously classified as pathogenic LGRs: the complete deletion of BRCA1 (identified in two unrelated families) and the deletion of exons 1 to 13. We also identified the duplication of exons 1 and 2 that is a LGR with unknown pathogenicity. Determination of the breakpoints of the BRCA1 LGRs using CNV/SNP arrays and sequencing identified them as NG_005905.2:g.70536_180359del, NG_005905.2:g.90012_97270dup, and NC_000017.10:g.41230935_41399840delinsAluSx1, respectively; previous observations of BRCA1 exon1-24del, exon1-2dup, and exon1-13del LGRs have not characterized them in such detail. All the BRCA1 LGRs arose from unequal homologous recombination events involving Alu elements. We also detected, by sequencing, one BRCA2 LGR, the Portuguese founder mutation c.156_157insAluYa5. The low frequency of BRCA1 LGRs within BRCA1 mutation carriers in Galicia (2.34%, 95% CI: 0.61-7.22) seems to differ from the Spanish population (9.93%, 95% CI: 6.76-14.27, P-value = 0.013) and from the rest of the Iberian population (9.76%, 95% CI: 6.69-13.94, P-value = 0.014).

  8. Contribution of nuclear analysis methods to the certification of BCR reference materials for non-metals in non-ferrous metals

    International Nuclear Information System (INIS)

    Pauwels, J.

    1979-01-01

    A number of reference materials for oxygen in different non-ferrous metals have been certified by BCR in the frame of a multidisciplinary Community project. The contribution of nuclear analysis methods is illustrated by several examples concerning the optimization of sample preparation techniques, the analysis of low and high oxygen non-ferrous metals and the extension of the program to other non-metals, especially nitrogen and carbon. (author)

  9. A Hybrid Approach to Proving Memory Reference Monotonicity

    KAUST Repository

    Oancea, Cosmin E.

    2013-01-01

    Array references indexed by non-linear expressions or subscript arrays represent a major obstacle to compiler analysis and to automatic parallelization. Most previous proposed solutions either enhance the static analysis repertoire to recognize more patterns, to infer array-value properties, and to refine the mathematical support, or apply expensive run time analysis of memory reference traces to disambiguate these accesses. This paper presents an automated solution based on static construction of access summaries, in which the reference non-linearity problem can be solved for a large number of reference patterns by extracting arbitrarily-shaped predicates that can (in)validate the reference monotonicity property and thus (dis)prove loop independence. Experiments on six benchmarks show that our general technique for dynamic validation of the monotonicity property can cover a large class of codes, incurs minimal run-time overhead and obtains good speedups. © 2013 Springer-Verlag.

  10. Analysis of flow pathway in a potential granitic host rock using Taiwan's K-area reference case data

    Science.gov (United States)

    Liou, Tai-Sheng; Kalatehjari, Roohollah; Tien, Neng-Chuan; Lin, Cheng-Kuo; Huang, Sung-Yang

    2017-04-01

    For low-permeability potential host rocks such as granite, the interconnected discrete fracture network (DFN) forms the fast preferential pathways for groundwater. Finding such pathways in fractured rocks thus becomes a vital technique for the final disposal of spent nuclear fuel. A series of techniques were developed in this study, including fracture data analysis, numerical simulation of DFN, upscaling of DFN into equivalent continuous porous medium (ECPM) and finally numerical simulation of brine in the upscaled ECPM. Investigation data were taken from Taiwan's K-area reference case Table II. Research activities implemented in K-area were for technology development purposes only. In other words, K-area has not been considered as a potential disposal site. Table II summaries the field data from preliminary investigations for the fundamental properties of the potential host rock. Data in Table II include fracture traces from outcrop mapping, borehole image fracture data and large-scale lineament. These data allowed us to analyze fracture intensity, fracture orientation and fracture size. At this preliminary investigation stage, it was decided to use empirical relationships between fracture size to fracture transmissivity as well as to fracture aperture. The deterministic major water-conducting fractures (MWCFs) in K-area have confirmed to be correlated to the Taiwushan fault and its branch fault. Besides the deterministic MWCFs, stochastic fractures were simulated by a DFN recipe that was modified from the original one in Table II. Numerical simulation of DFN and the calculation of its connected DFN were implemented by the commercial software FracMan V7.5. We have developed an upscaling code based on a volume-averaging method for converting a connected DFN into an ECPM. This upscaling code was successfully verified by benchmark examples. The upscaled ECPM is represented by a second-order, heterogeneous and anisotropic permeability tensor field. We also modified

  11. Whole Exome Sequencing and Segregation Analysis Confirms That a Mutation in COL17A1 Is the Cause of Epithelial Recurrent Erosion Dystrophy in a Large Dominant Pedigree Previously Mapped to Chromosome 10q23-q24.

    Directory of Open Access Journals (Sweden)

    Benjamin R Lin

    Full Text Available To report identification of a COL17A1 mutation in a family with a corneal dystrophy previously mapped to chromosome 10q23-q24.Whole-exome sequencing was performed on DNA samples from five affected family members and two unrelated, unaffected individuals. Identified variants were filtered for those that were: located in the linked interval on chromosome 10q23-q24; novel or rare (minor allele frequency ≤0.01; heterozygous; present in all affected individuals and not in controls; and present in genes that encode proteins expressed in human corneal epithelial cells (reads per kilobase per million ≥1. Sanger sequencing of identified variants (SNVs was performed in additional family members. In silico analysis was used to predict the functional impact of non-synonymous variants.Three SNVs located in two genes were identified that met the filtering criteria: one rare synonymous c.3156C>T variant in the collagen, type XVII, alpha I (COL17A1 gene; and two rare variants, one synonymous and one missense, in the dynamin binding protein (DNMBP gene. Sanger sequencing of additional family members determined that only the COL17A1 variant segregates with the affected phenotype. In silico analysis predicts that the missense variant in DNMBP would be tolerated.The corneal dystrophy mapped to chromosome 10q23-q24 is associated with the c.3156C>T variant in COL17A1. As this variant has recently been identified in five other families with early onset recurrent corneal erosions, and has been shown in vitro to introduce a cryptic splice donor site, this dystrophy is likely caused by aberrant splicing of COL17A1 and should be classified as epithelial recurrent erosion dystrophy.

  12. Analysis and comparison of NoSQL databases with an introduction to consistent references in big data storage systems

    Science.gov (United States)

    Dziedzic, Adam; Mulawka, Jan

    2014-11-01

    NoSQL is a new approach to data storage and manipulation. The aim of this paper is to gain more insight into NoSQL databases, as we are still in the early stages of understanding when to use them and how to use them in an appropriate way. In this submission descriptions of selected NoSQL databases are presented. Each of the databases is analysed with primary focus on its data model, data access, architecture and practical usage in real applications. Furthemore, the NoSQL databases are compared in fields of data references. The relational databases offer foreign keys, whereas NoSQL databases provide us with limited references. An intermediate model between graph theory and relational algebra which can address the problem should be created. Finally, the proposal of a new approach to the problem of inconsistent references in Big Data storage systems is introduced.

  13. The use of regression analysis in determining reference intervals for low hematocrit and thrombocyte count in multiple electrode aggregometry and platelet function analyzer 100 testing of platelet function.

    Science.gov (United States)

    Kuiper, Gerhardus J A J M; Houben, Rik; Wetzels, Rick J H; Verhezen, Paul W M; Oerle, Rene van; Ten Cate, Hugo; Henskens, Yvonne M C; Lancé, Marcus D

    2017-11-01

    Low platelet counts and hematocrit levels hinder whole blood point-of-care testing of platelet function. Thus far, no reference ranges for MEA (multiple electrode aggregometry) and PFA-100 (platelet function analyzer 100) devices exist for low ranges. Through dilution methods of volunteer whole blood, platelet function at low ranges of platelet count and hematocrit levels was assessed on MEA for four agonists and for PFA-100 in two cartridges. Using (multiple) regression analysis, 95% reference intervals were computed for these low ranges. Low platelet counts affected MEA in a positive correlation (all agonists showed r 2 ≥ 0.75) and PFA-100 in an inverse correlation (closure times were prolonged with lower platelet counts). Lowered hematocrit did not affect MEA testing, except for arachidonic acid activation (ASPI), which showed a weak positive correlation (r 2 = 0.14). Closure time on PFA-100 testing was inversely correlated with hematocrit for both cartridges. Regression analysis revealed different 95% reference intervals in comparison with originally established intervals for both MEA and PFA-100 in low platelet or hematocrit conditions. Multiple regression analysis of ASPI and both tests on the PFA-100 for combined low platelet and hematocrit conditions revealed that only PFA-100 testing should be adjusted for both thrombocytopenia and anemia. 95% reference intervals were calculated using multiple regression analysis. However, coefficients of determination of PFA-100 were poor, and some variance remained unexplained. Thus, in this pilot study using (multiple) regression analysis, we could establish reference intervals of platelet function in anemia and thrombocytopenia conditions on PFA-100 and in thrombocytopenia conditions on MEA.

  14. Is a Previously or Currently Reduced Testosterone Level in Male Patients with Type 2 Diabetes Mellitus a Risk Factor for the Development of Coronary Artery Disease? A Systematic Review and Meta-analysis.

    Science.gov (United States)

    Huang, Feng

    2018-04-04

    We aimed to systematically investigate the association between testosterone level and cardiovascular risk in male patients with type 2 diabetes mellitus (T2DM) by comparing male T2DM patients with low testosterone levels to male T2DM patients with normal testosterone levels at baseline in terms of the number of cases of coronary artery disease (CAD). Online databases were carefully searched for studies relating to testosterone, diabetes mellitus, and cardiovascular risk by using the search terms 'testosterone,' 'diabetes mellitus,' and 'coronary artery/cardiovascular disease.' The only endpoint in this analysis was CAD/atherosclerosis/coronary plaque/major adverse coronary event prior to or following testosterone reduction at baseline. The analytical parameters used in this analysis were the risk ratio (RR) with the 95% confidence interval (CI), as obtained using the RevMan 5.3 software. 3467 male patients with T2DM (1079 patients with a low testosterone level versus 2388 patients with a normal testosterone level) were included. The results of this analysis showed that when a random effects model was used, a higher risk of CAD/cardiovascular disease was observed in diabetic patients with a low testosterone level than in those with a normal testosterone level (RR 1.24, 95% CI 0.94-1.63; P = 0.13). However, the difference in risk was not statistically significant. Similarly, with a fixed effects model, a low testosterone level was associated with a higher CAD/cardiovascular risk of disease (RR 1.12, 95% CI 0.98-1.34; P = 0.08); in this case, the difference in risk between the T2DM patients with low and normal testosterone levels approached statistical significance. A previously or currently reduced testosterone level was not found to be statistically significantly associated with a high risk of CAD/cardiovascular disease in male patients with T2DM. However, the difference in risk between the T2DM patients with low and normal testosterone levels was observed

  15. Preparation and certification of the Polish reference material Virginia Tobacco Leaves (CTA-VTL-2) for inorganic trace analysis including microanalysis

    International Nuclear Information System (INIS)

    Dybczynski, R.; Polkowska-Motrenko, H.; Samczynski, Z.; Szopa, Z.

    1997-01-01

    A new Polish certified reference material Virginia Tobacco Leaves (CTA-VTL-2) for inorganic trace analysis including microanalysis has been prepared. Certification of the candidate reference material was based on the world-wide interlaboratory comparison in which 60 laboratories from 18 countries, participated using various analytical methods and techniques. Data evaluation performed by means of the new multifunctional software package -SSQC. Recommended values were assigned for 33 and 'information' values for 10 elements, respectively. The validity of 'certified' values was confirmed for several elements using 'very accurate' methods developed in this Laboratory. (author)

  16. Preparation and certification of the Polish reference material Virginia Tobacco Leaves (CTA-VTL-2) for inorganic trace analysis including microanalysis

    Energy Technology Data Exchange (ETDEWEB)

    Dybczynski, R.; Polkowska-Motrenko, H.; Samczynski, Z.; Szopa, Z.

    1997-12-31

    A new Polish certified reference material Virginia Tobacco Leaves (CTA-VTL-2) for inorganic trace analysis including microanalysis has been prepared. Certification of the candidate reference material was based on the world-wide interlaboratory comparison in which 60 laboratories from 18 countries, participated using various analytical methods and techniques. Data evaluation performed by means of the new multifunctional software package -SSQC. Recommended values were assigned for 33 and `information` values for 10 elements, respectively. The validity of `certified` values was confirmed for several elements using `very accurate` methods developed in this Laboratory. (author). 47 refs, 28 figs, 12 tabs.

  17. Blood pressure and fasting lipid changes after 24 weeks’ treatment with vildagliptin: a pooled analysis in >2,000 previously drug-naïve patients with type 2 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Evans M

    2016-08-01

    Full Text Available Marc Evans,1 Anja Schweizer,2 James E Foley3 1Diabetes Resource Centre, Llandough Hospital, Cardiff, UK; 2Medical Affairs Cardio Metabolic, Novartis Pharma AG, Basel, Switzerland; 3Medical Affairs Cardio-Metabolic, Novartis Pharmaceuticals Corporation, East Hanover, NJ, USA Introduction: We have previously shown modest weight loss with vildagliptin treatment. Since body weight balance is associated with changes in blood pressure (BP and fasting lipids, we have assessed these parameters following vildagliptin treatment. Methods: Data were pooled from all double-blind, randomized, controlled, vildagliptin monotherapy trials on previously drug-naïve patients with type 2 diabetes mellitus who received vildagliptin 50 mg once daily (qd or twice daily (bid; n=2,108 and wherein BP and fasting lipid data were obtained. Results: Data from patients receiving vildagliptin 50 mg qd or bid showed reductions from baseline to week 24 in systolic BP (from 132.5±0.32 to 129.8±0.34 mmHg; P<0.0001, diastolic BP (from 81.2±0.18 to 79.6±0.19 mmHg; P<0.0001, fasting triglycerides (from 2.00±0.02 to 1.80±0.02 mmol/L; P<0.0001, very low density lipoprotein cholesterol (from 0.90±0.01 to 0.83±0.01 mmol/L; P<0.0001, and low density lipoprotein cholesterol (from 3.17±0.02 to 3.04±0.02 mmol/L; P<0.0001, whereas high density lipoprotein cholesterol increased (from 1.19±0.01 to 1.22±0.01 mmol/L; P<0.001. Weight decreased by 0.48±0.08 kg (P<0.001. Conclusion: This large pooled analysis demonstrated that vildagliptin shows a significant reduction in BP and a favorable fasting lipid profile that are associated with modest weight loss. Keywords: TG, HDL, LDL, body weight DPP-4 inhibitor, GLP-1 

  18. Selective arsenic speciation analysis of human urine reference materials using gradient elution ion-exchange HPLC-ICP-MS

    DEFF Research Database (Denmark)

    Sloth, Jens Jørgen; Larsen, Erik Huusfeldt; Julshamn, K.

    2004-01-01

    identical with the reference values given for total arsenic. The obtained values for arsenobetaine and dimethylarsinic acid were identical with the values certified for the NIES No. 18 urine CRM. The speciation data presented here may be valuable for the quality assurance of analytical method development...

  19. The ultimate veal calf reference experiment: Hormone residue analysis data obtained by gas and liquid chromatography tandem mass spectrometry

    NARCIS (Netherlands)

    Nielen, M.W.F.; Lasaroms, J.J.P.; Essers, M.L.; Sanders, M.B.; Heskamp, H.H.; Bovee, T.F.H.; Rhijn, van J.A.; Groot, M.J.

    2007-01-01

    A lifetime controlled reference experiment has been performed using 42 veal calves, 21 males and 21 females which were fed and housed according to European regulations and common veterinary practice. During the experiment feed, water, urine and hair were sampled and feed intake and growth were

  20. Effect of long-term GH replacement therapy on cardiovascular outcomes in GH-deficient patients previously treated for acromegaly: a sub-analysis from the Dutch National Registry of Growth Hormone Treatment in Adults.

    Science.gov (United States)

    van Bunderen, Christa C; van Varsseveld, Nadège C; Heymans, Martijn W; Franken, Anton A M; Koppeschaar, Hans P F; van der Lely, Aart J; Drent, Madeleine L

    2014-12-01

    The effect of GH deficiency (GHD) on the metabolic profile of acromegaly patients is unclear in patients previously treated for acromegaly, as are the efficacy and safety of GH treatment in this particular group. The aim of the study is to describe the characteristics of patients with severe GHD who were previously treated for acromegaly, and to investigate the effects of long-term GH treatment on cardiovascular risk factors and morbidity, compared with patients who were treated for a nonfunctioning pituitary adenoma (NFPA). A nationwide surveillance study. Sixty-five patients from the Dutch National Registry of Growth Hormone Treatment in Adults with previous acromegaly were compared with 778 patients with previous NFPA. Cardiovascular indices, including body composition, lipid profile, glucose metabolism, blood pressure, and morbidity were investigated. GHD patients with previous acromegaly had an unfavorable metabolic profile comparable with or more than GHD patients with previous NFPA. GH treatment led to improvement of the lipid profile in both groups, also after excluding patients using lipid-lowering medication. In patients with previous acromegaly, HbA1c levels increased more than in patients with previous NFPA (estimate 0.03, 95% CI 0.002-0.06, P=0.04). The risk for developing cardiovascular diseases was not different between the groups. The patients with GHD after previous acromegaly have an unfavorable metabolic profile comparable with patients with GHD after previous NFPA. In both groups, the lipid profile improves during GH treatment. Changes in glucose metabolism should be monitored closely. GH treatment in patients with GHD previously treated for acromegaly had no deleterious effect on cardiovascular morbidity. © 2014 European Society of Endocrinology.

  1. Establishment of safety analysis system for emergency operating procedures of Kori 3 and 4 and YGN 1 and 2 and reference analyses

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Won Jae; Kim, Hee Cheol; Ha, Kwi Seok; Chung, Bub Dong; Jeong, Jae Jun

    1999-02-01

    This report describes the establishment of safety analysis system for emergency operating procedures(EOPs) of Kori 3 and 4 and YGN 1 and 2 and the results of reference analyses. MARS1.3.1 code has been selected as a realistic system analysis tool of the safety analysis system, and the reactor system has been modeled using a fine nodding scheme in order to capture the major thermal-hydraulic phenomena that might occur during the transients. Full power steady state operating conditions are generated based on the plant operation data. Then, the reference analyses have been carried out for the accidents that can represent the typical EOPtransients, that is, a small-break loss-of-coolant-accident,a main steam line break accident and a steam generator tube rupture accident from the full power operation. For the realistic simulation of plant transient responses, reactor control and protection systems and safety systems are modeled based on their realistic performances. Also, the operator actions are modeled based on the current EOP actions. Through the reference analyses, the soundness of the established safety analysis system and the system modeling has been verified and the effectiveness of the current EOP has been partly justified. In conclusion, the safety analysis system established through this study can be used for the generation of technical background in the development and improvement of EOP actions and in the operator training. (Author). 11 refs., 6 tabs., 48 figs.

  2. Selection and validation of reference genes for gene expression analysis in switchgrass (Panicum virgatum) using quantitative real-time RT-PCR.

    Science.gov (United States)

    Gimeno, Jacinta; Eattock, Nicholas; Van Deynze, Allen; Blumwald, Eduardo

    2014-01-01

    Switchgrass (Panicum virgatum) has received a lot of attention as a forage and bioenergy crop during the past few years. Gene expression studies are in progress to improve new traits and develop new cultivars. Quantitative real time PCR (qRT-PCR) has emerged as an important technique to study gene expression analysis. For accurate and reliable results, normalization of data with reference genes is essential. In this work, we evaluate the stability of expression of genes to use as reference for qRT-PCR in the grass P. virgatum. Eleven candidate reference genes, including eEF-1α, UBQ6, ACT12, TUB6, eIF-4a, GAPDH, SAMDC, TUA6, CYP5, U2AF, and FTSH4, were validated for qRT-PCR normalization in different plant tissues and under different stress conditions. The expression stability of these genes was verified by the use of two distinct algorithms, geNorm and NormFinder. Differences were observed after comparison of the ranking of the candidate reference genes identified by both programs but eEF-1α, eIF-4a, CYP5 and U2AF are ranked as the most stable genes in the samples sets under study. Both programs discard the use of SAMDC and TUA6 for normalization. Validation of the reference genes proposed by geNorm and NormFinder were performed by normalization of transcript abundance of a group of target genes in different samples. Results show similar expression patterns when the best reference genes selected by both programs were used but differences were detected in the transcript abundance of the target genes. Based on the above research, we recommend the use of different statistical algorithms to identify the best reference genes for expression data normalization. The best genes selected in this study will help to improve the quality of gene expression data in a wide variety of samples in switchgrass.

  3. Cryptococcal meningitis in a previously healthy child | Chimowa ...

    African Journals Online (AJOL)

    An 8-year-old previously healthy female presented with a 3 weeks history of headache, neck stiffness, deafness, fever and vomiting and was diagnosed with cryptococcal meningitis. She had documented hearing loss and was referred to tertiary-level care after treatment with fluconazole did not improve her neurological ...

  4. Primary angioplasty vs. fibrinolysis in very old patients with acute myocardial infarction: TRIANA (TRatamiento del Infarto Agudo de miocardio eN Ancianos) randomized trial and pooled analysis with previous studies.

    Science.gov (United States)

    Bueno, Héctor; Betriu, Amadeo; Heras, Magda; Alonso, Joaquín J; Cequier, Angel; García, Eulogio J; López-Sendón, José L; Macaya, Carlos; Hernández-Antolín, Rosana

    2011-01-01

    To compare primary percutaneous coronary intervention (pPCI) and fibrinolysis in very old patients with ST-segment elevation myocardial infarction (STEMI), in whom head-to-head comparisons between both strategies are scarce. Patients ≥75 years old with STEMI <6 h were randomized to pPCI or fibrinolysis. The primary endpoint was a composite of all-cause mortality, re-infarction, or disabling stroke at 30 days. The trial was prematurely stopped due to slow recruitment after enrolling 266 patients (134 allocated to pPCI and 132 to fibrinolysis). Both groups were well balanced in baseline characteristics. Mean age was 81 years. The primary endpoint was reached in 25 patients in the pPCI group (18.9%) and 34 (25.4%) in the fibrinolysis arm [odds ratio (OR), 0.69; 95% confidence interval (CI) 0.38-1.23; P = 0.21]. Similarly, non-significant reductions were found in death (13.6 vs. 17.2%, P = 0.43), re-infarction (5.3 vs. 8.2%, P = 0.35), or disabling stroke (0.8 vs. 3.0%, P = 0.18). Recurrent ischaemia was less common in pPCI-treated patients (0.8 vs. 9.7%, P< 0.001). No differences were found in major bleeds. A pooled analysis with the two previous reperfusion trials performed in older patients showed an advantage of pPCI over fibrinolysis in reducing death, re-infarction, or stroke at 30 days (OR, 0.64; 95% CI 0.45-0.91). Primary PCI seems to be the best reperfusion therapy for STEMI even for the oldest patients. Early contemporary fibrinolytic therapy may be a safe alternative to pPCI in the elderly when this is not available.

  5. Evaluation of the reference genes for expression analysis using quantitative real-time polymerase chain reaction in the green peach aphid, Myzus persicae.

    Science.gov (United States)

    Kang, Zhi-Wei; Liu, Fang-Hua; Tian, Hong-Gang; Zhang, Meng; Guo, Shan-Shan; Liu, Tong-Xian

    2017-04-01

    The green peach aphid, Myzus persicae Sulzer (Hemiptera, Aphididae), is an important cosmopolitan pest. Real time qRT-PCR has been used for target gene expression analysis on M. persicae. Using real time qRT-PCR, the expression levels are normalized on the basis of the reliable reference genes. However, to date, the stability of available reference genes has been insufficient. In this study, we evaluated nine candidate reference genes from M. persicae under diverse experimental conditions. The tested candidate genes were comprehensively ranked based on five alternative methods (RefFinder, geNorm, Normfinder, BestKeeper and the comparative ΔC t method). 18s, Actin and ribosomal protein L27 (L27) were recommended as the most stable reference genes for M. persicae, whereas ribosomal protein L27 (L27) was found to be the least stable reference genes for abiotic studies (photoperiod, temperature and insecticide susceptibility). Our finding not only sheds light on establishing an accurate and reliable normalization of real time qRT-PCR data in M. persicae but also lays a solid foundation for further studies of M. persicae involving RNA interference and functional gene research. © 2016 Institute of Zoology, Chinese Academy of Sciences.

  6. Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.

    Science.gov (United States)

    Wu, Bin-Yan; Ye, Jian-Ren; Huang, Lin; He, Ling-Min; Li, De-Wei

    2017-01-01

    Burkholderia pyrrocinia strain JK-SH007 isolated from poplar stems plays a highly significant role in the growth promotion and the biocontrol of poplar canker during colonization in poplar. In this research, the ideal reference gene was filtered and determined for the transcript normalization. Additionally, the expression of pyrG under all four conditions was relatively stable in B. pyrrocinia JK-SH007. Copyright © 2016. Published by Elsevier B.V.

  7. Analysis and compensation of reference frequency mismatch in multiple-frequency feedforward active noise and vibration control system

    Science.gov (United States)

    Liu, Jinxin; Chen, Xuefeng; Yang, Liangdong; Gao, Jiawei; Zhang, Xingwu

    2017-11-01

    In the field of active noise and vibration control (ANVC), a considerable part of unwelcome noise and vibration is resulted from rotational machines, making the spectrum of response signal multiple-frequency. Narrowband filtered-x least mean square (NFXLMS) is a very popular algorithm to suppress such noise and vibration. It has good performance since a priori-knowledge of fundamental frequency of the noise source (called reference frequency) is adopted. However, if the priori-knowledge is inaccurate, the control performance will be dramatically degraded. This phenomenon is called reference frequency mismatch (RFM). In this paper, a novel narrowband ANVC algorithm with orthogonal pair-wise reference frequency regulator is proposed to compensate for the RFM problem. Firstly, the RFM phenomenon in traditional NFXLMS is closely investigated both analytically and numerically. The results show that RFM changes the parameter estimation problem of the adaptive controller into a parameter tracking problem. Then, adaptive sinusoidal oscillators with output rectification are introduced as the reference frequency regulator to compensate for the RFM problem. The simulation results show that the proposed algorithm can dramatically suppress the multiple-frequency noise and vibration with an improved convergence rate whether or not there is RFM. Finally, case studies using experimental data are conducted under the conditions of none, small and large RFM. The shaft radial run-out signal of a rotor test-platform is applied to simulate the primary noise, and an IIR model identified from a real steel structure is applied to simulate the secondary path. The results further verify the robustness and effectiveness of the proposed algorithm.

  8. The manufacture and analysis of a reference defect for high frequency ultrasonic inspections of CANDU pressure tubes

    International Nuclear Information System (INIS)

    Graf, M.A.

    1990-01-01

    Ultrasonic inspection has long been used to monitor CANDU reactor pressure tubes both during their manufacture and in service. Conventional 10 MHz inspection techniques have missed some inclusion-type defects, which were later discovered using high-frequency (100 MHz) techniques. The routine use of high-frequency techniques is being considered. A reference defect is needed to demonstrate the effectiveness and accuracy of this technique. A reference defect was manufactured by inserting small metallic inclusions along the radial centre line of a pressure tube wall. Two equal-length sections of tube were cut and machined so the inner diameter of one was equal to the outer diameter of the other. Sample defects in the form of thin shims and wires were placed on the outer surface of the smaller tube. The larger tube was fitted around it. The tubes were bonded together using the HIP (hot isostatic press) process. All of the defects were detectable at 10 MHz, and therefore the reference defect was not suitable to evaluate high-frequency techniques. Defects at least an order of magnitude smaller should be used. Cylindrical defects tend to be preferable to layered geometries from the point of view of their transparency to lower frequency signals. (L.L.) (3 refs., 4 figs.)

  9. Performance analysis of PPP ambiguity resolution with UPD products estimated from different scales of reference station networks

    Science.gov (United States)

    Wang, Siyao; Li, Bofeng; Li, Xingxing; Zang, Nan

    2018-01-01

    Integer ambiguity fixing with uncalibrated phase delay (UPD) products can significantly shorten the initialization time and improve the accuracy of precise point positioning (PPP). Since the tracking arcs of satellites and the behavior of atmospheric biases can be very different for the reference networks with different scales, the qualities of corresponding UPD products may be also various. The purpose of this paper is to comparatively investigate the influence of different scales of reference station networks on UPD estimation and user ambiguity resolution. Three reference station networks with global, wide-area and local scales are used to compute the UPD products and analyze their impact on the PPP-AR. The time-to-first-fix, the unfix rate and the incorrect fix rate of PPP-AR are analyzed. Moreover, in order to further shorten the convergence time for obtaining precise positioning, a modified partial ambiguity resolution (PAR) and corresponding validation strategy are presented. In this PAR method, the ambiguity subset is determined by removing the ambiguity one by one in the order of ascending elevations. Besides, for static positioning mode, a coordinate validation strategy is employed to enhance the reliability of the fixed coordinate. The experiment results show that UPD products computed by smaller station network are more accurate and lead to a better coordinate solution; the PAR method used in this paper can shorten the convergence time and the coordinate validation strategy can improve the availability of high precision positioning.

  10. Reference-free quantification of EEG spectra: combining current source density (CSD) and frequency principal components analysis (fPCA).

    Science.gov (United States)

    Tenke, Craig E; Kayser, Jürgen

    2005-12-01

    Definition of appropriate frequency bands and choice of recording reference limit the interpretability of quantitative EEG, which may be further compromised by distorted topographies or inverted hemispheric asymmetries when employing conventional (non-linear) power spectra. In contrast, fPCA factors conform to the spectral structure of empirical data, and a surface Laplacian (2-dimensional CSD) simplifies topographies by minimizing volume-conducted activity. Conciseness and interpretability of EEG and CSD fPCA solutions were compared for three common scaling methods. Resting EEG and CSD (30 channels, nose reference, eyes open/closed) from 51 healthy and 93 clinically-depressed adults were simplified as power, log power, and amplitude spectra, and summarized using unrestricted, Varimax-rotated, covariance-based fPCA. Multiple alpha factors were separable from artifact and reproducible across subgroups. Power spectra produced numerous, sharply-defined factors emphasizing low frequencies. Log power spectra produced fewer, broader factors emphasizing high frequencies. Solutions for amplitude spectra showed optimal intermediate tuning, particularly when derived from CSD rather than EEG spectra. These solutions were topographically distinct, detecting multiple posterior alpha generators but excluding the dorsal surface of the frontal lobes. Instead a low alpha/theta factor showed a secondary topography along the frontal midline. CSD amplitude spectrum fPCA solutions provide simpler, reference-independent measures that more directly reflect neuronal activity. A new quantitative EEG approach affording spectral components is developed that closely parallels the concept of an ERP component in the temporal domain.

  11. Analysis of variance, normal quantile-quantile correlation and effective expression support of pooled expression ratio of reference genes for defining expression stability

    Directory of Open Access Journals (Sweden)

    Himanshu Priyadarshi

    2017-01-01

    Full Text Available Identification of a reference gene unaffected by the experimental conditions is obligatory for accurate measurement of gene expression through relative quantification. Most existing methods directly analyze variability in crossing point (Cp values of reference genes and fail to account for template-independent factors that affect Cp values in their estimates. We describe the use of three simple statistical methods namely analysis of variance (ANOVA, normal quantile-quantile correlation (NQQC and effective expression support (EES, on pooled expression ratios of reference genes in a panel to overcome this issue. The pooling of expression ratios across the genes in the panel nullify the sample specific effects uniformly affecting all genes that are falsely reflected as instability. Our methods also offer the flexibility to include sample specific PCR efficiencies in estimations, when available, for improved accuracy. Additionally, we describe a correction factor from the ANOVA method to correct the relative fold change of a target gene if no truly stable reference gene could be found in the analyzed panel. The analysis is described on a synthetic data set to simplify the explanation of the statistical treatment of data.

  12. A análise seminal deve ser requisitada para homens com histórico de fertilidade prévia? Should semen analysis be requested for men with a history of previous fertility?

    Directory of Open Access Journals (Sweden)

    Eleonora Bedin Pasqualotto

    2006-11-01

    ão devem presumir que um paciente possui uma análise seminal normal, baseados no fato de este possuir história de estabelecimento de uma gravidez no passado.PURPOSE: to determine if the previous fertility history can predict current fertility status of a patient examined for couple’s infertility. METHODS: retrospective study involving semen analyses from 183 consecutive subfertile patients evaluated from September 2002 to March 2004. We excluded those patients who had undergone radio or chemotherapy, orchiectomy or vasectomy. Mean values of all analyses were used for patients with multiple semen analysis. Patients with more than 20x10(6 sperm/mL, motility higher than 50% and with normal strict sperm morphology higher than 14% were considered normal. Patients were divided into two groups, according to the fertility status: primary infertility (118 patients and secondary infertility (65 patients. Data were analyzed according to the chi2 test and the Student t-test. RESULTS: no differences were detected in the mean age between patients with primary infertility, 37.3±6.3, and secondary infertility, 38.1±5.9; p=0.08. In the group of patients with primary infertility, 51.9% (61 patients had a normal sperm concentration, 70.3% (83 patients had normal sperm motility and 26.3% (31 patients had normal sperm morphology. In the group of patients with secondary infertility, 53.8% (35 patients had normal sperm concentration, 75.4% (49 patients had normal sperm motility and 32.3% (21 patients had normal sperm morphology. No significant differences were detected in sperm concentration (21.3x10(6/mL versus 23.1x10(6/mL; p=0.07, motility (45.2 versus 48.1%; p=0.08 and morphology (6.1 versus 6.4%; p=0.09 between groups of patients with primary and secondary infertility. CONCLUSIONS: semen analysis should be requested even in cases of prior male fertility. Physicians should not presume a patient to have a normal semen analysis based on his previous history of initiating a pregnancy.

  13. Space shuttle engineering and operations support. ALT separation reference trajectories for tailcone on orbiter forward and aft CG configurations. Mission planning, mission analysis and software formulation

    Science.gov (United States)

    Glenn, G. M.

    1977-01-01

    A preflight analysis of the ALT separation reference trajectories for the tailcone on, forward, and aft cg orbiter configurations is documented. The ALT separation reference trajectories encompass the time from physical separation of the orbiter from the carrier to orbiter attainment of the maximum ALT interface airspeed. The trajectories include post separation roll maneuvers by both vehicles and are generated using the final preflight data base. The trajectories so generated satisfy all known separation design criteria and violate no known constraints. The requirement for this analysis is given along with the specifications, assumptions, and analytical approach used to generate the separation trajectories. The results of the analytical approach are evaluated, and conclusions and recommendations are summarized.

  14. A meta-analysis of echocardiographic measurements of the left heart for the development of normative reference ranges in a large international cohort

    DEFF Research Database (Denmark)

    Møgelvang, Rasmus

    2014-01-01

    and 95th centile of each measurement against age. CONCLUSION: This unique data set represents a large, multi-ethnic cohort of subjects resident in a wide range of countries. The resultant reference ranges will have wide applicability for normative data based on age, sex, and ethnicity.......AIM: To develop age-, sex-, and ethnic-appropriate normative reference ranges for standard echocardiographic measurements of the left heart by combining echocardiographic measurements obtained from adult volunteers without clinical cardiovascular disease or significant cardiovascular risk factors......, from multiple studies around the world. METHODS AND RESULTS: The Echocardiographic Normal Ranges Meta-Analysis of the Left heart (EchoNoRMAL) collaboration was established and population-based data sets of echocardiographic measurements combined to perform an individual person data meta-analysis. Data...

  15. Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE), Version 5.0: Models and Results Database (MAR-D) reference manual. Volume 8

    International Nuclear Information System (INIS)

    Russell, K.D.; Skinner, N.L.

    1994-07-01

    The Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) refers to a set of several microcomputer programs that were developed to create and analyze probabilistic risk assessments (PRAs), primarily for nuclear power plants. The primary function of MAR-D is to create a data repository for completed PRAs and Individual Plant Examinations (IPEs) by providing input, conversion, and output capabilities for data used by IRRAS, SARA, SETS, and FRANTIC software. As probabilistic risk assessments and individual plant examinations are submitted to the NRC for review, MAR-D can be used to convert the models and results from the study for use with IRRAS and SARA. Then, these data can be easily accessed by future studies and will be in a form that will enhance the analysis process. This reference manual provides an overview of the functions available within MAR-D and step-by-step operating instructions

  16. Preparation and characterization of a new set of IAEA reference air filters using instrumental neutron activation analysis, proton-induced X-ray emission and Rutherford backscattering

    Czech Academy of Sciences Publication Activity Database

    Kučera, Jan; Havránek, Vladimír; Krausová, Ivana

    2009-01-01

    Roč. 281, č. 1 (2009), s. 123-129 ISSN 0236-5731. [9th International Conference on Nuclear Analytical Methods in the Life Sciences. Lisbon, 07.09.2008-12.09.2008] Institutional research plan: CEZ:AV0Z10480505 Keywords : Reference air filters * instrumental neutron activation analysis * Proton induced X-ray emission Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders Impact factor: 0.631, year: 2009

  17. Construction and analysis of the NCI-EDRN breast cancer reference set for circulating markers of disease.

    Science.gov (United States)

    Marks, Jeffrey R; Anderson, Karen S; Engstrom, Paul; Godwin, Andrew K; Esserman, Laura J; Longton, Gary; Iversen, Edwin S; Mathew, Anu; Patriotis, Christos; Pepe, Margaret S

    2015-02-01

    Many circulating biomarkers have been reported for the diagnosis of breast cancer, but few, if any, have undergone rigorous credentialing using prospective cohorts and blinded evaluation. The NCI Early Detection Research Network (EDRN) has created a prospective, multicenter collection of plasma and serum samples from 832 subjects designed to evaluate circulating biomarkers for the detection and diagnosis of breast cancer. These samples are available to investigators who wish to evaluate their biomarkers using a set of blinded samples. The breast cancer reference set is composed of blood samples collected using a standard operating procedure at four U.S. medical centers from 2008 to 2010 from women undergoing either tissue diagnosis for breast cancer or routine screening mammography. The reference set contains samples from women with incident invasive cancer (n = 190), carcinoma in situ (n = 55), benign pathology with atypia (n = 63), benign disease with no atypia (n = 231), and women with no evidence of breast disease by screening mammography (BI-RADS 1 or 2, n = 276). Using a subset of plasma samples (n = 505) from the reference set, we analyzed 90 proteins by multiplexed immunoassays for their potential utility as diagnostic markers. We found that none of these markers is useful for distinguishing cancer from benign controls. However, elevated CA-125 does appear to be a candidate marker for estrogen receptor-negative cancers. Markers that can distinguish benign breast conditions from invasive cancer have not yet been found. Availability of prospectively collected samples should improve future validation efforts. ©2014 American Association for Cancer Research.

  18. Development of a new ferulic acid certified reference material for use in clinical chemistry and pharmaceutical analysis

    Directory of Open Access Journals (Sweden)

    Dezhi Yang

    2015-05-01

    Full Text Available This study compares the results of three certified methods, namely differential scanning calorimetry (DSC, the mass balance (MB method and coulometric titrimetry (CT, in the purity assessment of ferulic acid certified reference material (CRM. Purity and expanded uncertainty as determined by the three methods were respectively 99.81%, 0.16%; 99.79%, 0.16%; and 99.81%, 0.26% with, in all cases, a coverage factor (k of 2 (P=95%. The purity results are consistent indicating that the combination of DSC, the MB method and CT provides a confident assessment of the purity of suitable CRMs like ferulic acid.

  19. The Reference Encounter Model.

    Science.gov (United States)

    White, Marilyn Domas

    1983-01-01

    Develops model of the reference interview which explicitly incorporates human information processing, particularly schema ideas presented by Marvin Minsky and other theorists in cognitive processing and artificial intelligence. Questions are raised concerning use of content analysis of transcribed verbal protocols as methodology for studying…

  20. Reference Gene Selection for qPCR Analysis in Tomato-Bipartite Begomovirus Interaction and Validation in Additional Tomato-Virus Pathosystems.

    Directory of Open Access Journals (Sweden)

    Ana L M Lacerda

    Full Text Available Quantitative Polymerase Chain Reaction (qPCR is currently the most sensitive technique used for absolute and relative quantification of a target gene transcript, requiring the use of appropriated reference genes for data normalization. To accurately estimate the relative expression of target tomato (Solanum lycopersicum L. genes responsive to several virus species in reverse transcription qPCR analysis, the identification of reliable reference genes is mandatory. In the present study, ten reference genes were analyzed across a set of eight samples: two tomato contrasting genotypes ('Santa Clara', susceptible, and its near-isogenic line 'LAM 157', resistant; subjected to two treatments (inoculation with Tomato chlorotic mottle virus (ToCMoV and its mock-inoculated control and in two distinct times after inoculation (early and late. Reference genes stability was estimated by three statistical programs (geNorm, NormFinder and BestKeeper. To validate the results over broader experimental conditions, a set of ten samples, corresponding to additional three tomato-virus pathosystems that included tospovirus, crinivirus and tymovirus + tobamovirus, was analyzed together with the tomato-ToCMoV pathosystem dataset, using the same algorithms. Taking into account the combined analyses of the ranking order outputs from the three algorithms, TIP41 and EF1 were identified as the most stable genes for tomato-ToCMoV pathosystem, and TIP41 and EXP for the four pathosystems together, and selected to be used as reference in the forthcoming expression qPCR analysis of target genes in experimental conditions involving the aforementioned tomato-virus pathosystems.

  1. International collaborative study of the endogenous reference gene, sucrose phosphate synthase (SPS), used for qualitative and quantitative analysis of genetically modified rice.

    Science.gov (United States)

    Jiang, Lingxi; Yang, Litao; Zhang, Haibo; Guo, Jinchao; Mazzara, Marco; Van den Eede, Guy; Zhang, Dabing

    2009-05-13

    One rice ( Oryza sativa ) gene, sucrose phosphate synthase (SPS), has been proven to be a suitable endogenous reference gene for genetically modified (GM) rice detection in a previous study. Herein are the reported results of an international collaborative ring trial for validation of the SPS gene as an endogenous reference gene and its optimized qualitative and quantitative polymerase chain reaction (PCR) systems. A total of 12 genetically modified organism (GMO) detection laboratories from seven countries participated in the ring trial and returned their results. The validated results confirmed the species specificity of the method through testing 10 plant genomic DNAs, low heterogeneity, and a stable single-copy number of the rice SPS gene among 7 indica varieties and 5 japonica varieties. The SPS qualitative PCR assay was validated with a limit of detection (LOD) of 0.1%, which corresponded to about 230 copies of haploid rice genomic DNA, while the limit of quantification (LOQ) for the quantitative PCR system was about 23 copies of haploid rice genomic DNA, with acceptable PCR efficiency and linearity. Furthermore, the bias between the test and true values of eight blind samples ranged from 5.22 to 26.53%. Thus, we believe that the SPS gene is suitable for use as an endogenous reference gene for the identification and quantification of GM rice and its derivates.

  2. Staffing an Academic Reference Desk with Librarians is not Cost-effective. A Review of: Ryan, Susan M. “Reference Transactions Analysis: The Cost-effectiveness of Staffing a Traditional Academic Reference Desk.” Journal of Academic Librarianship 34.5 (2008: 389-99.

    Directory of Open Access Journals (Sweden)

    Cari Merkley

    2009-06-01

    Full Text Available Objective – To determine whether it is cost effective to staff an academic reference desk with librarians through an examination of the types of reference questions being asked and the qualifications required to answer them.Design – Content analysis of reference transaction logs and activity-based costing for reference services based on quantitative data derived from the logs.Setting – Stetson University, a private institution in the United States with an FTE of approximately 2500.Subjects – 6959 phone, email, and in-person reference transactions logged at the reference desk by four full-time and two part-time librarians.Methods – This study repurposes data originally collected to determine the frequency with which librarians turned to online versus print sources when responding to questions at the reference desk. Librarians working at the Stetson University library reference desk recorded all reference queries received in person, by phone, or by email for a total of eight months between 2002 and 2006. Data collection took place in two month intervals in fall 2002, spring 2003, spring 2006, and fall 2006. Each question and the sources used to address it were logged by the librarian. Directional questions that were not related to the library’s collections and technical questions dealing with printer or copier mechanical problems were counted, but the specifics of these questions were not recorded. It was felt that these queries would not yield data relevant to the original research question on sources used as they “did not directly relate to an information need” (391. A total of 6959 questions were logged by librarians during the four collection periods. Questions were recorded for only 4431 transactions; the remaining 2528 queries related to printer/copier problems or non-library specific directions and were described as “direction and machine: non- informational” (394. The 4431 recorded questions were then divided into four

  3. Selection of reference genes for RT-qPCR analysis in a predatory biological control agent, Coleomegilla maculata (Coleoptera: Coccinellidae).

    Science.gov (United States)

    Yang, Chunxiao; Pan, Huipeng; Noland, Jeffrey Edward; Zhang, Deyong; Zhang, Zhanhong; Liu, Yong; Zhou, Xuguo

    2015-12-10

    Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for quantifying gene expression across various biological processes, of which requires a set of suited reference genes to normalize the expression data. Coleomegilla maculata (Coleoptera: Coccinellidae), is one of the most extensively used biological control agents in the field to manage arthropod pest species. In this study, expression profiles of 16 housekeeping genes selected from C. maculata were cloned and investigated. The performance of these candidates as endogenous controls under specific experimental conditions was evaluated by dedicated algorithms, including geNorm, Normfinder, BestKeeper, and ΔCt method. In addition, RefFinder, a comprehensive platform integrating all the above-mentioned algorithms, ranked the overall stability of these candidate genes. As a result, various sets of suitable reference genes were recommended specifically for experiments involving different tissues, developmental stages, sex, and C. maculate larvae treated with dietary double stranded RNA. This study represents the critical first step to establish a standardized RT-qPCR protocol for the functional genomics research in a ladybeetle C. maculate. Furthermore, it lays the foundation for conducting ecological risk assessment of RNAi-based gene silencing biotechnologies on non-target organisms; in this case, a key predatory biological control agent.

  4. Nuclear energy cost data base: A reference data base for nuclear and coal-fired powerplant power generation cost analysis

    International Nuclear Information System (INIS)

    1988-09-01

    A reference data base and standard methodology are needed for performing comparative nuclear and fossil power generation cost analyses for the Department of Energy, Office of Nuclear Energy. This report contains such a methodology together with reference assumptions and data to be used with the methodology. It is intended to provide basic guidelines or a starting point for analyses and to serve as a focal point in establishing parameters and methods to be used in economic comparisons of nuclear systems with alternatives. The data base is applicable for economic comparisons of new base load light-water reactors on a once-through cycle, and high- and low-sulfur coal-fired plants, and oil- and natural gas-fired electric generating plants coming on line around the turn of the century. In addition to current generation light-water reactors and fossil fuel-fired plants, preliminary cost information is also presented on improved and advanced light-water reactors, liquid metal reactor plants and fuel cycle facilities. This report includes an updated data base containing proposed technical and economic assumptions to be used in analyses, discussions of a recommended methodology to be used in calculating power generation costs, a sample calculation for illustrative and benchmark purposes and projected power generation costs for fission and coal-fired alternatives. Effects of the 1986 Tax Reform Act are included. 126 refs., 17 figs., 47 tabs

  5. Nuclear Energy Cost Data Base: a reference data base for nuclear and coal-fired powerplant power generation cost analysis

    International Nuclear Information System (INIS)

    1985-06-01

    A reference data base and standard methodology are needed for performing comparative nuclear and fossil power generation cost analyses for the Department of Energy, Office of Nuclear Energy. This report contains such a methodology together with reference assumptions and data to be used with the methodology. It is intended to provide basic guidelines or a starting point for analyses and to serve as a focal point in establishing parameters and methods to be used in economic comparisons of nuclear systems with alternatives. The data base is applicable for economic comparisons of new base load light-water reactors on either the current once-through cycle or self-generated recycle, high- and low-sulfur coal-fired plants, and oil- and natural gas-fired electric generating plants coming on line in the last decade of this century. In addition to light-water reactors and fossil fuel-fired plants, preliminary cost information is also presented on liquid metal reactor plants. This report includes a data base containing proposed technical and economic assumptions to be used in analyses, discussions of a recommended methodology to be used in calculating power generation costs, and a sample calculation for illustrative and benchmark purposes

  6. Nuclear Energy Cost Data Base: A reference data base for nuclear and coal-fired powerplant power generation cost analysis

    International Nuclear Information System (INIS)

    Delene, J.G.; Bowers, H.I.

    1986-12-01

    A reference data base and standard methodology are needed for performing comparative nuclear and fossil power generation cost analyses for the Department of Energy, Office of Nuclear Energy. This report contains such a methodology together with reference assumptions and data to be used with the methodology. It is intended to provide basic guidelines or a starting point for analyses and to serve as a focal point in establishing parameters and methods to be used in economic comparisons of nuclear systems with alternatives. The data base is applicable for economic comparisons of new base load light-water reactors on either the current once-through cycle or self-generated recycle, high- and low-sulfur coal-fired plants, and oil- and natural gas-fired electric generating plants coming on line around the turn of the century. In additions to light-water reactors and fossil fuel-fired plants, preliminary cost information is also presented on liquid metal reactor plants. This report includes a data base containing proposed technical and economic assumptions to be used in analyses, discussions of recommended methodology to be used in calculating power generation costs, and a sample calculation for illustrative benchmark purposes

  7. Rates of induced abortion in Denmark according to age, previous births and previous abortions

    Directory of Open Access Journals (Sweden)

    Marie-Louise H. Hansen

    2009-11-01

    Full Text Available Background: Whereas the effects of various socio-demographic determinants on a woman's risk of having an abortion are relatively well-documented, less attention has been given to the effect of previous abortions and births. Objective: To study the effect of previous abortions and births on Danish women's risk of an abortion, in addition to a number of demographic and personal characteristics. Data and methods: From the Fertility of Women and Couples Dataset we obtained data on the number of live births and induced abortions by year (1981-2001, age (16-39, county of residence and marital status. Logistic regression analysis was used to estimate the influence of the explanatory variables on the probability of having an abortion in a relevant year. Main findings and conclusion: A woman's risk of having an abortion increases with the number of previous births and previous abortions. Some interactions were was found in the way a woman's risk of abortion varies with calendar year, age and parity. The risk of an abortion for women with no children decreases while the risk of an abortion for women with children increases over time. Furthermore, the risk of an abortion decreases with age, but relatively more so for women with children compared to childless women. Trends for teenagers are discussed in a separate section.

  8. MinION Analysis and Reference Consortium: Phase 2 data release and analysis of R9.0 chemistry [version 1; referees: 1 approved, 2 approved with reservations

    Directory of Open Access Journals (Sweden)

    Miten Jain

    2017-05-01

    Full Text Available Background: Long-read sequencing is rapidly evolving and reshaping the suite of opportunities for genomic analysis. For the MinION in particular, as both the platform and chemistry develop, the user community requires reference data to set performance expectations and maximally exploit third-generation sequencing. We performed an analysis of MinION data derived from whole genome sequencing of Escherichia coli K-12 using the R9.0 chemistry, comparing the results with the older R7.3 chemistry. Methods: We computed the error-rate estimates for insertions, deletions, and mismatches in MinION reads. Results: Run-time characteristics of the flow cell and run scripts for R9.0 were similar to those observed for R7.3 chemistry, but with an 8-fold increase in bases per second (from 30 bps in R7.3 and SQK-MAP005 library preparation, to 250 bps in R9.0 processed by individual nanopores, and less drop-off in yield over time. The 2-dimensional (“2D” N50 read length was unchanged from the prior chemistry. Using the proportion of alignable reads as a measure of base-call accuracy, 99.9% of “pass” template reads from 1-dimensional (“1D”  experiments were mappable and ~97% from 2D experiments. The median identity of reads was ~89% for 1D and ~94% for 2D experiments. The total error rate (miscall + insertion + deletion decreased for 2D “pass” reads from 9.1% in R7.3 to 7.5% in R9.0 and for template “pass” reads from 26.7% in R7.3 to 14.5% in R9.0. Conclusions: These Phase 2 MinION experiments serve as a baseline by providing estimates for read quality, throughput, and mappability. The datasets further enable the development of bioinformatic tools tailored to the new R9.0 chemistry and the design of novel biological applications for this technology. Abbreviations: K: thousand, Kb: kilobase (one thousand base pairs, M: million, Mb: megabase (one million base pairs, Gb: gigabase (one billion base pairs.

  9. Selection of reference genes for gene expression analysis by real-time qPCR in avian cells infected with infectious bronchitis virus.

    Science.gov (United States)

    Batra, Ambalika; Maier, Helena J; Fife, Mark S

    2017-04-01

    Infectious bronchitis virus (IBV) causes infectious bronchitis in poultry, a respiratory disease that is a source of major economic loss to the poultry industry. Detection and the study of the molecular pathogenesis of the virus often involve the use of real-time quantitative PCR assays (qPCR). To account for error within the experiments, the levels of target gene transcription are normalized to that of suitable reference genes. Despite publication of the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines in 2009, single un-tested reference genes are often used for normalization of qPCR assays in avian research studies. Here, we use the geNorm algorithm to identify suitable reference genes in different avian cell types during infection with apathogenic and pathogenic strains of IBV. We discuss the importance of selecting an appropriate experimental sample subset for geNorm analysis, and show the effect that this selection can have on resultant reference gene selection. The effects of inappropriate normalization on the transcription pattern of a cellular signalling gene, AKT1, and the interferon-inducible, MX1, were studied. We identify the possibility of the misinterpretation of qPCR data when an inappropriate normalization strategy is employed. This is most notable when measuring the transcription of AKT1, where changes are minimal during infection.

  10. Enterprise Reference Library

    Science.gov (United States)

    Bickham, Grandin; Saile, Lynn; Havelka, Jacque; Fitts, Mary

    2011-01-01

    Introduction: Johnson Space Center (JSC) offers two extensive libraries that contain journals, research literature and electronic resources. Searching capabilities are available to those individuals residing onsite or through a librarian s search. Many individuals have rich collections of references, but no mechanisms to share reference libraries across researchers, projects, or directorates exist. Likewise, information regarding which references are provided to which individuals is not available, resulting in duplicate requests, redundant labor costs and associated copying fees. In addition, this tends to limit collaboration between colleagues and promotes the establishment of individual, unshared silos of information The Integrated Medical Model (IMM) team has utilized a centralized reference management tool during the development, test, and operational phases of this project. The Enterprise Reference Library project expands the capabilities developed for IMM to address the above issues and enhance collaboration across JSC. Method: After significant market analysis for a multi-user reference management tool, no available commercial tool was found to meet this need, so a software program was built around a commercial tool, Reference Manager 12 by The Thomson Corporation. A use case approach guided the requirements development phase. The premise of the design is that individuals use their own reference management software and export to SharePoint when their library is incorporated into the Enterprise Reference Library. This results in a searchable user-specific library application. An accompanying share folder will warehouse the electronic full-text articles, which allows the global user community to access full -text articles. Discussion: An enterprise reference library solution can provide a multidisciplinary collection of full text articles. This approach improves efficiency in obtaining and storing reference material while greatly reducing labor, purchasing and

  11. Preparation and certification of two new bulk welding fume reference materials for use in laboratories undertaking analysis of occupational hygiene samples.

    Science.gov (United States)

    Butler, Owen; Musgrove, Darren; Stacey, Peter

    2014-01-01

    Workers can be exposed to fume, arising from welding activities, which contain toxic metals and metalloids. Occupational hygienists need to assess and ultimately minimize such exposure risks. The monitoring of the concentration of particles in workplace air is one assessment approach whereby fume, from representative welding activities, is sampled onto a filter and returned to a laboratory for analysis. Inductively coupled plasma-atomic emission spectrometry and inductively coupled plasma-mass spectrometry are generally employed as instrumental techniques of choice for the analysis of such filter samples. An inherent difficulty, however, with inductively coupled plasma-based analytical techniques is that they typically require a sample to be presented for analysis in the form of a solution. The efficiency of the required dissolution step relies heavily upon the skill and experience of the analyst involved. A useful tool in assessing the efficacy of this dissolution step would be the availability and subsequent analysis of welding fume reference materials with stated elemental concentrations and matrices that match as closely as possible the matrix composition of welding fume samples submitted to laboratories for analysis. This article describes work undertaken at the Health and Safety Laboratory to prepare and certify two new bulk welding fume reference materials that can be routinely used by analysts to assess the performance of the digestion procedures they employ in their laboratories.

  12. Engineering education research in European Journal of Engineering Education and Journal of Engineering Education: citation and reference discipline analysis

    Science.gov (United States)

    Wankat, Phillip C.; Williams, Bill; Neto, Pedro

    2014-01-01

    The authors, citations and content of European Journal of Engineering Education (EJEE) and Journal of Engineering Education (JEE) in 1973 (JEE, 1975 EJEE), 1983, 1993, 2003, and available 2013 issues were analysed. Both journals transitioned from house organs to become engineering education research (EER) journals, although JEE transitioned first. In this process the number of citations rose, particularly of education and psychology sources; the percentage of research articles increased markedly as did the number of reference disciplines. The number of papers per issue, the number of single author papers, and the citations of science and engineering sources decreased. EJEE has a very broad geographic spread of authors while JEE authors are mainly US based. A 'silo' mentality where general engineering education researchers do not communicate with EER researchers in different engineering disciplines is evident. There is some danger that EER may develop into a silo that does not communicate with technically oriented engineering professors.

  13. Mapping VIPS concepts for nursing interventions to the ISO reference terminology model for nursing actions: A collaborative Scandinavian analysis

    DEFF Research Database (Denmark)

    Ehnfors, Margareta; Angermo, Lilly Marit; Berring, Lene

    2006-01-01

    The aims of this study were to analyze the coherence between the concepts for nursing interventions in the Swedish VIPS model for nursing recording and the ISO Reference Terminology Model for Nursing Actions and to identify areas in the two models for further development. Seven Scandinavian experts....... In most cases, the recipient of care is implicit. Expressions for the aim of an action are absent from the ISO model. By this mapping we identified areas for future development of the VIPS model and the experience from nursing terminology work in Scandinavia can contribute to the international...... analyzed the VIPS model's concepts for nursing interventions using prototypical examples of nursing actions, involving 233 units of analyses, and collaborated in mapping the two models. All nursing interventions in the VIPS model comprise actions and targets, but a few lack explicit expressions of means...

  14. Mapping VIPS Concepts for Nursing Interventions to the ISO Reference Terminology Model for Nursing Actions: A Collaborative Scandinavian Analysis

    DEFF Research Database (Denmark)

    Lauge Berring, Lene; Ehnfors, Margareta; Angermo, Lilly

    2005-01-01

    The aims of this study were to analyze the coherence between the concepts for nursing interventions in the Swedish VIPS model for nursing recording and the ISO Reference Terminology Model for Nursing Actions and to identify areas in the two models for further development. Seven Scandinavian experts....... In most cases, the recipient of care is implicit. Expressions for the aim of an action are absent from the ISO model. By this mapping we identified areas for future development of the VIPS model and the experience from nursing terminology work in Scandinavia can contribute to the international...... analyzed the VIPS model's concepts for nursing interventions using prototypical examples of nursing actions, involving 233 units of analyses, and collaborated in mapping the two models. All nursing interventions in the VIPS model comprise actions and targets, but a few lack explicit expressions of means...

  15. Identification of novel reference genes using multiplatform expression data and their validation for quantitative gene expression analysis.

    Directory of Open Access Journals (Sweden)

    Mi Jeong Kwon

    Full Text Available Normalization of mRNA levels using endogenous reference genes (ERGs is critical for an accurate comparison of gene expression between different samples. Despite the popularity of traditional ERGs (tERGs such as GAPDH and ACTB, their expression variability in different tissues or disease status has been reported. Here, we first selected candidate housekeeping genes (HKGs using human gene expression data from different platforms including EST, SAGE, and microarray, and 13 novel ERGs (nERGs (ARL8B, CTBP1, CUL1, DIMT1L, FBXW2, GPBP1, LUC7L2, OAZ1, PAPOLA, SPG21, TRIM27, UBQLN1, ZNF207 were further identified from these HKGs. The mean coefficient variation (CV values of nERGs were significantly lower than those of tERGs and the expression level of most nERGs was relatively lower than high expressing tERGs in all dataset. The higher expression stability and lower expression levels of most nERGs were validated in 108 human samples including formalin-fixed paraffin-embedded (FFPE tissues, frozen tissues and cell lines, through quantitative real-time RT-PCR (qRT-PCR. Furthermore, the optimal number of nERGs required for accurate normalization was as few as two, while four genes were required when using tERGs in FFPE tissues. Most nERGs identified in this study should be better reference genes than tERGs, based on their higher expression stability and fewer numbers needed for normalization when multiple ERGs are required.

  16. Stimuli previously associated with reinforcement mitigate resurgence.

    Science.gov (United States)

    Craig, Andrew R; Browning, Kaitlyn O; Shahan, Timothy A

    2017-09-01

    Resurgence refers to the recurrence of an extinguished target behavior following subsequent suspension of alternative reinforcement. Delivery of reinforcers during extinction of alternative behavior has been shown to mitigate resurgence. The present experiment aimed to determine whether delivering stimuli associated with reinforcers during resurgence testing similarly mitigates resurgence. Three groups of rats pressed target levers for food according to variable-interval 15-s schedules during Phase 1. In Phase 2, lever pressing was extinguished, and an alternative nose-poke response produced alternative reinforcement according to a variable-interval 15-s schedule. Food reinforcement was always associated with illumination of the food aperture and an audible click from the pellet dispenser during Phases 1 and 2. Phase 3 treatments differed between groups. For one group, nose poking continued to produce food and food-correlated stimuli. Both of these consequences were suspended for a second group. Finally, nose poking produced food-correlated stimuli but not food for a third group. Target-lever pressing resurged in the group that received no consequences and in the group that received only food-correlated stimuli for nose poking. Resurgence, however, was smaller for the group that received food-correlated stimuli than for the group that received no consequences for nose poking. Target-lever pressing did not increase between phases in the group that continued to receive food and associated stimuli. Thus, delivery of stimuli associated with food reinforcement after suspension of food reduced but did not eliminate resurgence of extinguished lever pressing. These findings contribute to potential methodologies for preventing relapse of extinguished problem behavior in clinical settings. © 2017 Society for the Experimental Analysis of Behavior.

  17. DAKOTA : a multilevel parallel object-oriented framework for design optimization, parameter estimation, uncertainty quantification, and sensitivity analysis. Version 5.0, user's reference manual.

    Energy Technology Data Exchange (ETDEWEB)

    Eldred, Michael Scott; Dalbey, Keith R.; Bohnhoff, William J.; Adams, Brian M.; Swiler, Laura Painton; Hough, Patricia Diane (Sandia National Laboratories, Livermore, CA); Gay, David M.; Eddy, John P.; Haskell, Karen H.

    2010-05-01

    The DAKOTA (Design Analysis Kit for Optimization and Terascale Applications) toolkit provides a flexible and extensible interface between simulation codes and iterative analysis methods. DAKOTA contains algorithms for optimization with gradient and nongradient-based methods; uncertainty quantification with sampling, reliability, and stochastic finite element methods; parameter estimation with nonlinear least squares methods; and sensitivity/variance analysis with design of experiments and parameter study methods. These capabilities may be used on their own or as components within advanced strategies such as surrogate-based optimization, mixed integer nonlinear programming, or optimization under uncertainty. By employing object-oriented design to implement abstractions of the key components required for iterative systems analyses, the DAKOTA toolkit provides a flexible and extensible problem-solving environment for design and performance analysis of computational models on high performance computers. This report serves as a reference manual for the commands specification for the DAKOTA software, providing input overviews, option descriptions, and example specifications.

  18. DAKOTA, a multilevel parallel object-oriented framework for design optimization, parameter estimation, uncertainty quantification, and sensitivity analysis:version 4.0 reference manual

    Energy Technology Data Exchange (ETDEWEB)

    Griffin, Joshua D. (Sandai National Labs, Livermore, CA); Eldred, Michael Scott; Martinez-Canales, Monica L. (Sandai National Labs, Livermore, CA); Watson, Jean-Paul; Kolda, Tamara Gibson (Sandai National Labs, Livermore, CA); Adams, Brian M.; Swiler, Laura Painton; Williams, Pamela J. (Sandai National Labs, Livermore, CA); Hough, Patricia Diane (Sandai National Labs, Livermore, CA); Gay, David M.; Dunlavy, Daniel M.; Eddy, John P.; Hart, William Eugene; Guinta, Anthony A.; Brown, Shannon L.

    2006-10-01

    The DAKOTA (Design Analysis Kit for Optimization and Terascale Applications) toolkit provides a flexible and extensible interface between simulation codes and iterative analysis methods. DAKOTA contains algorithms for optimization with gradient and nongradient-based methods; uncertainty quantification with sampling, reliability, and stochastic finite element methods; parameter estimation with nonlinear least squares methods; and sensitivity/variance analysis with design of experiments and parameter study methods. These capabilities may be used on their own or as components within advanced strategies such as surrogate-based optimization, mixed integer nonlinear programming, or optimization under uncertainty. By employing object-oriented design to implement abstractions of the key components required for iterative systems analyses, the DAKOTA toolkit provides a flexible and extensible problem-solving environment for design and performance analysis of computational models on high performance computers. This report serves as a reference manual for the commands specification for the DAKOTA software, providing input overviews, option descriptions, and example specifications.

  19. Comparing the role of standard references on the prevalence of Iranian children and adolescents' overweight and obesity: A systematic review and meta-analysis.

    Science.gov (United States)

    Ghanbari, Saeed; Ayatollahi, Seyyed Mohammad Taghi

    2016-01-01

    Obesity is a major risk factor for chronic diseases and has a role on high blood pressure, diabetes type II, etc., This review assesses the prevalence of Iranian children obesity and overweight for different age categories and compares the three standard definitions of obesity. To retrieve desirable studies concerning childhood anthropometric data from different area of Iran, the MEDLINE, Scopus, and different local databases such as Scientific Information database were used. The studies reported the prevalence of obesity or overweight of children references which are the Centers for Disease Control and Prevention (CDC), the World Health Organization (WHO) definition, and the International Obesity Task Force (IOTF) references. The analysis was carried out using STATA software. Our review covered 75 articles reported the prevalence of overweight or obesity among children and adolescents for different age groups in Iran. Our meta-regression analysis showed that the prevalence of obesity and overweight did not vary significantly in gender and age categories, but different definitions provide different prevalence of overweight and obesity. The effective factors on obesity and overweight included administration policy and organizational, interpersonal, intrapersonal, and social factors. CDC and WHO references intended in monitoring children's growth and the IOTF cutoffs would rather provide a common set of definitions that researchers and policymakers could use for descriptive and comparative purposes.

  20. Chemical Data Reporting - Previously Collected Data

    Science.gov (United States)

    EPA now refers to the Inventory Update Reporting (IUR) rule as the Chemical Data Reporting (CDR) Rule. This change was effective with the publication of the Inventory Update Reporting Modifications; Chemical Data Reporting Final Rule in August 2011.

  1. Protocolo de Observação Comportamental - PROC: valores de referência para uma análise quantitativa Behavioral Observation Protocol: reference values for a quantitative analysis

    Directory of Open Access Journals (Sweden)

    Simone Rocha de Vasconcellos Hage

    2012-08-01

    Full Text Available OBJETIVO: obter valores de referência para protocolo de observação comportamental (PROC sobre o desenvolvimento de habilidades comunicativas e de esquemas simbólicos em crianças com desenvolvimento típico de linguagem. MÉTODO: foram avaliadas 44 crianças entre 24 e 47 meses de ambos os gêneros, selecionadas em escolas de educação infantil, por meio de triagem do desenvolvimento global Denver II e questionário com os pais. Todas as crianças foram filmadas durante 30 minutos em interação com um adulto em atividade envolvendo brinquedos. As gravações foram analisadas por meio do PROC. A análise estatística descreveu valores de média, mediana, valores mínimos e máximos. Foi utilizado o teste T de Student para comparação das idades. RESULTADOS: nas habilidades comunicativas, as crianças do estudo mostraram evolução com a idade (média para três e dois anos, respectivamente: 58,12 e 51,44, apesar de não ter sido encontrada diferença estatisticamente significante para as faixas etárias comparadas (p=0,486. Quanto ao item compreensão verbal, as crianças de três anos obtiveram melhor desempenho que as de dois (respectivas médias: 59,41 e 50,70, havendo diferença estatisticamente significante (p=0,0000020. Em relação ao item aspectos do desenvolvimento cognitivo, as crianças de três anos apresentaram melhor desempenho em comparação com as de dois (respectivas médias: 44,53 e 31,96, havendo diferença estatisticamente significante entre as pontuações obtidas (p=0,00364, mostrando que as crianças evoluem na hierarquia do simbolismo. CONCLUSÃO: a obtenção de valores de referência para o PROC veio combinar análise qualitativa e quantitativa, contribuindo, além do diagnóstico, para o acompanhamento objetivo de processos terapêuticos.PURPOSE: to obtain reference values for behavioral observation protocol on the development of communicative and symbolic schemes of children with typical language development

  2. Reference materials for quality assurance in sea-water analysis: performance of total-reflection x-ray fluorescence in the intercomparison and certification stages

    International Nuclear Information System (INIS)

    Freimann, P.; Schmidt, D.; Neubauer-Ziebarth, A.

    1993-01-01

    The certification of a sea-water reference material (CRM 403) was completed by the Community Bureau of Reference (BCR) of the Commission of the European Communities during an intercomparison exercise. A feasibility study on estuarine water was undertaken. In both studies total-reflection X-ray fluorescence analysis (TXRF) was used for the determination of V, Mn, Fe, Co, Ni, Cu, Zn, Pb and U on the nmol/kg level. The enrichment of the trace metals and the separation from the salt matrix were performed by complexation with sodium dibenzyldithiocarbamate and reverse-phase chromatography. In this paper, the high performance of our TXRF results is compared to other analytical techniques like voltammetric and atomic absorption methods. (author)

  3. Comparative analysis of a hypothetical coolant loss accident in an LMFB reactor with the use of various calculation models for a common reference problem

    International Nuclear Information System (INIS)

    Royl, P.

    1979-01-01

    The results of a comparative analysis of the initial and dismantling stages of a hypothetical loss of flow accident in an LMFB reactor are presented. The analyses were made for a common reference problem with four different calculation models (CARMEN/KADIS, SURDYN, CAPRI/KADIS and FRAX). The reference core is described specifically, as are the differences in its geometrical disposition in the models, the static and transient conditions before and after the start of boiling and during dismantling. The differences in the models used for simulating the boiling and the dismantling are compared. The structure of the core, as well as the calculation conditions and hypotheses, were intentionally designed so that the accident would culminate, in all cases, in an energetic hydrodynamic dismantling stage

  4. Metonymy and reference-point errors in novice programming

    Science.gov (United States)

    Miller, Craig S.

    2014-07-01

    When learning to program, students often mistakenly refer to an element that is structurally related to the element that they intend to reference. For example, they may indicate the attribute of an object when their intention is to reference the whole object. This paper examines these reference-point errors through the context of metonymy. Metonymy is a rhetorical device where the speaker states a referent that is structurally related to the intended referent. For example, the following sentence states an office bureau but actually refers to a person working at the bureau: The tourist asked the travel bureau for directions to the museum. Drawing upon previous studies, I discuss how student reference errors may be consistent with the use of metonymy. In particular, I hypothesize that students are more likely to reference an identifying element even when a structurally related element is intended. I then present two experiments, which produce results consistent with this analysis. In both experiments, students are more likely to produce reference-point errors that involve identifying attributes than descriptive attributes. Given these results, I explore the possibility that students are relying on habits of communication rather than the mechanistic principles needed for successful programming. Finally I discuss teaching interventions using live examples and how metonymy may be presented to non-computing students as pedagogy for computational thinking.

  5. [Analysis of serum lipids levels and the establishment of reference intervals for serum lipids in middle and late pregnancy].

    Science.gov (United States)

    Ying, Chunmei; Yue, Chaoyan; Zhang, Chunyi; Li, Xiaotian

    2015-12-01

    To explore the serum lipids levels in healthy pregnant women, and to establish the reference intervals of serum lipids in middle and late pregnancy. Triglyceride (TG), total cholesterol (TCH), high density lipoprotein (HDL), low density lipoprotein (LDL), apo-lipoprotein-A(APO-A) and apo-lipoprotein-B (APO-B) were measured in 3 200 pregnant women and 3 200 healthy women of childbearing age(the control group) from January 2014 to Febuary 2015 in Obstetrics and Gynecology Hospital of Fudan University. In the healthy pregnant women, serum lipids were measured at 14-20, 24-28 and 37-40 gestational weeks, respectively. All the parameters were detected by Hitachi 7180 automatic biochemical analyzer. The test results were calculated and determined by the C28-A3 standard of the National Clinical and Laboratory Standards Institute. And the normal reference intervals of serum lipids in middle and late pregnancy were defined as 2.5%-97.5%. (1) The levels of TG, TCH, HDL, LDL, APO-A and APO-B in the control group were 0.8, 4.2, 1.0, 2.7 mmol/L and 1.1, 0.8 g/L, respectively. The levels of TG, TCH, HDL, LDL, APO-A and APO-B in middle and late pregnancy were significantly higher than those in the control group (Plipids levels at 14-20, 24-28 and 37-40 gestational weeks in healthy pregnant women were compared with the control group as following. The TG levels were 1.9, 3.8 and 4.4 folds of the control group; the TCH levels were 1.1, 1.5 and 1.5 folds of the control group; the HDL levels were 1.2, 1.6 and 1.5 folds of the control group; the LDL levels were 1.1, 1.4 and 1.4 folds of the control group; the APO-A levels were 1.3, 1.4 and 1.5 folds of the control group; and the APO-B levels were 1.1, 1.5 and 1.5 fold of the control group respectively. The TG level was the most increased, and it increased gradually with gestational age (Plipids at 14-20, 24-28 and 37-40 gestational weeks in healthy pregnant women were as following. The TG levels were 0.7-3.9, 1.7-6.3 and 1.6-8.1 mmol

  6. Toward Optimal Decision Making among Vulnerable Patients Referred for Cardiac Surgery: A Qualitative Analysis of Patient and Provider Perspectives.

    Science.gov (United States)

    Gainer, Ryan A; Curran, Janet; Buth, Karen J; David, Jennie G; Légaré, Jean-Francois; Hirsch, Gregory M

    2017-07-01

    Comprehension of risks, benefits, and alternative treatment options has been shown to be poor among patients referred for cardiac interventions. Patients' values and preferences are rarely explicitly sought. An increasing proportion of frail and older patients are undergoing complex cardiac surgical procedures with increased risk of both mortality and prolonged institutional care. We sought input from patients and caregivers to determine the optimal approach to decision making in this vulnerable patient population. Focus groups were held with both providers and former patients. Three focus groups were convened for Coronary Artery Bypass Graft (CABG), Valve, or CABG +Valve patients ≥ 70 y old (2-y post-op, ≤ 8-wk post-op, complicated post-op course) (n = 15). Three focus groups were convened for Intermediate Medical Care Unit (IMCU) nurses, Intensive Care Unit (ICU) nurses, surgeons, anesthesiologists and cardiac intensivists (n = 20). We used a semi-structured interview format to ask questions surrounding the informed consent process. Transcribed audio data was analyzed to develop consistent and comprehensive themes. We identified 5 main themes that influence the decision making process: educational barriers, educational facilitators, patient autonomy and perceived autonomy, patient and family expectations of care, and decision making advocates. All themes were influenced by time constraints experienced in the current consent process. Patient groups expressed a desire to receive information earlier in their care to allow time to identify personal values and preferences in developing plans for treatment. Both groups strongly supported a formal approach for shared decision making with a decisional coach to provide information and facilitate communication with the care team. Identifying the barriers and facilitators to patient and caretaker engagement in decision making is a key step in the development of a structured, patient-centered SDM approach. Intervention

  7. Evaluation of a new electronic preoperative reference marker for toric intraocular lens implantation by two different methods of analysis: Adobe Photoshop versus iTrace.

    Science.gov (United States)

    Farooqui, Javed Hussain; Sharma, Mansi; Koul, Archana; Dutta, Ranjan; Shroff, Noshir Minoo

    2017-01-01

    The aim of this study is to compare two different methods of analysis of preoperative reference marking for toric intraocular lens (IOL) after marking with an electronic marker. Cataract and IOL Implantation Service, Shroff Eye Centre, New Delhi, India. Fifty-two eyes of thirty patients planned for toric IOL implantation were included in the study. All patients had preoperative marking performed with an electronic preoperative two-step toric IOL reference marker (ASICO AE-2929). Reference marks were placed at 3-and 9-o'clock positions. Marks were analyzed with two systems. First, slit-lamp photographs taken and analyzed using Adobe Photoshop (version 7.0). Second, Tracey iTrace Visual Function Analyzer (version 5.1.1) was used for capturing corneal topograph examination and position of marks noted. Amount of alignment error was calculated. Mean absolute rotation error was 2.38 ± 1.78° by Photoshop and 2.87 ± 2.03° by iTrace which was not statistically significant ( P = 0.215). Nearly 72.7% of eyes by Photoshop and 61.4% by iTrace had rotation error ≤3° ( P = 0.359); and 90.9% of eyes by Photoshop and 81.8% by iTrace had rotation error ≤5° ( P = 0.344). No significant difference in absolute amount of rotation between eyes when analyzed by either method. Difference in reference mark positions when analyzed by two systems suggests the presence of varying cyclotorsion at different points of time. Both analysis methods showed an approximately 3° of alignment error, which could contribute to 10% loss of astigmatic correction of toric IOL. This can be further compounded by intra-operative marking errors and final placement of IOL in the bag.

  8. Placental complications after a previous cesarean section

    OpenAIRE

    Milošević Jelena; Lilić Vekoslav; Tasić Marija; Radović-Janošević Dragana; Stefanović Milan; Antić Vladimir

    2009-01-01

    Introduction The incidence of cesarean section has been rising in the past 50 years. With the increased number of cesarean sections, the number of pregnancies with the previous cesarean section rises as well. The aim of this study was to establish the influence of the previous cesarean section on the development of placental complications: placenta previa, placental abruption and placenta accreta, as well as to determine the influence of the number of previous cesarean sections on the complic...

  9. Reference genes for accessing differential expression among developmental stages and analysis of differential expression of OBP genes in Anastrepha obliqua

    Science.gov (United States)

    Nakamura, Aline Minali; Chahad-Ehlers, Samira; Lima, André Luís A.; Taniguti, Cristiane Hayumi; Sobrinho Jr., Iderval; Torres, Felipe Rafael; de Brito, Reinaldo Alves

    2016-01-01

    The West Indian fruit fly, Anastrepha obliqua, is an important agricultural pest in the New World. The use of pesticide-free methods to control invasive species such as this reinforces the search for genes potentially useful in their genetic control. Therefore, the study of chemosensory proteins involved with a range of responses to the chemical environment will help not only on the understanding of the species biology but may also help the development of environmentally friendly pest control strategies. Here we analyzed the expression patterns of three OBP genes, Obp19d_2, Obp56a and Obp99c, across different phases of A. obliqua development by qPCR. In order to do so, we tested eight and identified three reference genes for data normalization, rpl17, rpl18 and ef1a, which displayed stability for the conditions here tested. All OBPs showed differential expression on adults and some differential expression among adult stages. Obp99c had an almost exclusive expression in males and Obp56a showed high expression in virgin females. Thereby, our results provide relevant data not only for other gene expression studies in this species, as well as for the search of candidate genes that may help in the development of new pest control strategies. PMID:26818909

  10. Comparison between a new computer program and the reference software for gray-scale median analysis of atherosclerotic carotid plaques.

    Science.gov (United States)

    Casella, Ivan Benaduce; Fukushima, Rodrigo Bono; Marques, Anita Battistini de Azevedo; Cury, Marcus Vinícius Martins; Presti, Calógero

    2015-03-01

    To compare a new dedicated software program and Adobe Photoshop for gray-scale median (GSM) analysis of B-mode images of carotid plaques. A series of 42 carotid plaques generating ≥50% diameter stenosis was evaluated by a single observer. The best segment for visualization of internal carotid artery plaque was identified on a single longitudinal view and images were recorded in JPEG format. Plaque analysis was performed by both programs. After normalization of image intensity (blood = 0, adventitial layer = 190), histograms were obtained after manual delineation of plaque. Results were compared with nonparametric Wilcoxon signed rank test and Kendall tau-b correlation analysis. GSM ranged from 00 to 100 with Adobe Photoshop and from 00 to 96 with IMTPC, with a high grade of similarity between image pairs, and a highly significant correlation (R = 0.94, p < .0001). IMTPC software appears suitable for the GSM analysis of carotid plaques. © 2014 Wiley Periodicals, Inc.

  11. Preparation and certification of certified reference materials JAERI-Z21, Z22 and Z23 for analysis of zirconium and its alloys

    International Nuclear Information System (INIS)

    Takashima, Kyoichiro

    1991-03-01

    The Sub-Committee on Chemical Analysis of Nuclear Materials was organized in April 1987, under the Committee on Analytical Chemistry of Nuclear Fuels and Reactor Materials, JAERI, for renewal of certified reference materials of zirconium base alloys and zirconium metal. Collaborative analysis was carried out among ten participating laboratories for the certification of the JAERI CRMs Z21 to Z23. As a results of the collaborative works, the certified values for sixteen elements (Sn, Fe, Ni, Cr, Hf, Al, Si, Co, Cu, Ti, Mn, Pb, U, Cd, B and W) in the CRMs were given. In this report, preparation of raw materials, homogeneity test, chemical analysis for certification by collaborative works during April 1987 to March 1990 are described. (author)

  12. Development of Certified Matrix-Based Reference Material as a Calibrator for Genetically Modified Rice G6H1 Analysis.

    Science.gov (United States)

    Yang, Yu; Li, Liang; Yang, Hui; Li, Xiaying; Zhang, Xiujie; Xu, Junfeng; Zhang, Dabing; Jin, Wujun; Yang, Litao

    2018-04-03

    The accurate monitoring and quantification of genetically modified organisms (GMOs) are key points for the implementation of labeling regulations, and a certified reference material (CRM) acts as the scaleplate for quantifying the GM contents of foods/feeds and evaluating a GMO analytical method or equipment. Herein we developed a series of CRMs for transgenic rice event G6H1, which possesses insect-resistant and herbicide-tolerant traits. Three G6H1 CRMs were produced by mixing seed powders obtained from homozygous G6H1 and its recipient cultivar Xiushui 110 at mass ratios of 49.825%, 9.967%, and 4.986%. The between-bottle homogeneity and within-bottle homogeneity were thoroughly evaluated with consistent results. The potential DNA degradation in transportation and shelf life were evaluated with an expiration period of at least 12 months. The property values of three CRMs (G6H1 a , G6H1 b , G6H1 c ) were given as (49.825 ± 0.448) g/kg, (9.967 ± 1.757) g/kg, and (4.986 ± 1.274 g/kg based on mass fraction ratio, respectively. Furthermore, the three CRMs were characterized with values of (5.01 ± 0.08)%, (1.06 ± 0.22)%, and (0.53 ± 0.11)% based on the copy number ratio using the droplet digital PCR method. All results confirmed that the produced G6H1 matrix-based CRMs are of high quality with precise characterization values and can be used as calibrators in GM rice G6H1 inspection and monitoring and in evaluating new analytical methods or devices targeting the G6H1 event.

  13. Comparative analysis of biofilm formation by Bacillus cereus reference strains and undomesticated food isolates and the effect of free iron.

    Science.gov (United States)

    Hayrapetyan, Hasmik; Muller, Lisette; Tempelaars, Marcel; Abee, Tjakko; Nierop Groot, Masja

    2015-05-04

    Biofilm formation of Bacillus cereus reference strains ATCC 14579 and ATCC 10987 and 21 undomesticated food isolates was studied on polystyrene and stainless steel as contact surfaces. For all strains, the biofilm forming capacity was significantly enhanced when in contact with stainless steel (SS) as a surface as compared to polystyrene (PS). For a selection of strains, the total CFU and spore counts in biofilms were determined and showed a good correlation between CFU counts and total biomass of these biofilms. Sporulation was favoured in the biofilm over the planktonic state. To substantiate whether iron availability could affect B. cereus biofilm formation, the free iron availability was varied in BHI by either the addition of FeCl3 or by depletion of iron with the scavenger 2,2-Bipyridine. Addition of iron resulted in increased air-liquid interface biofilm on polystyrene but not on SS for strain ATCC 10987, while the presence of Bipyridine reduced biofilm formation for both materials. Biofilm formation was restored when excess FeCl3 was added in combination with the scavenger. Further validation of the iron effect for all 23 strains in microtiter plate showed that fourteen strains (including ATCC10987) formed a biofilm on PS. For eight of these strains biofilm formation was enhanced in the prese