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Sample records for preparation certification evaluation

  1. AutoCAD 2014 review for certification official certification preparation

    CERN Document Server

    ASCENT center for technical knowledge

    2014-01-01

    The AutoCAD® 2014 Review for Certification book is intended for users of AutoCAD® preparing to complete the AutoCAD 2014 Certified Professional exam. This book contains a collection of relevant instructional topics, practice exercises, and review questions from the Autodesk Official Training Guides (AOTG) from ASCENT - Center for Technical Knowledge pertaining specifically to the Certified Professional exam topics and objectives. This book is intended for experienced users of AutoCAD in preparation for certification. New users of AutoCAD should refer to the AOTG training guides from ASCENT, such as AutoCAD/AutoCAD LT 2014 Fundamentals, for more comprehensive instruction.

  2. The Effect of Certification and Preparation on Teacher Quality

    Science.gov (United States)

    Boyd, Donald; Goldhaber, Daniel; Lankford, Hamilton; Wyckoff, James

    2007-01-01

    To improve the quality of the teacher workforce, some states have tightened teacher preparation and certification requirements while others have eased requirements and introduced "alternative" ways of being certified to attract more people to teaching. Donald Boyd, Daniel Goldhaber, Hamilton Lankford, and James Wyckoff evaluate these seemingly…

  3. PREPARATION OF CERTIFICATES BY GENERAL SURGERY RESIDENT PHYSICIANS

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    Carlos Lazzarino (h

    2014-04-01

    Full Text Available The physician’s certificate is a statement regarding a present or past event in which the physician has intervened while exercising his/her profession. It is considered written and personal evidence of the attending physician, which verifies the event described. Official certificates are those which must be issued in compliance with legal provisions, while ordinary certificates are private documents prepared at the request of the patient himself/herself. 88 certificates issued by general surgery resident physicians at the patient’s request were assessed, between October and December 2013. All the ordinary certificates assessed were aimed at controlling absenteeism. They all included the patient’s name and surname, date, signature and seal. The preparation of a physicians’ certificate is mandatory, and should only include the facts verified by the physician himself/herself, and upon the patient’s request. General surgery resident physicians are duly licensed medical doctors, which means that they are empowered by law to issue certificates, verifications and/or statements made in the course of their profession, regarding conditions of health or illness, as well as the management, prescription, indication, application or control of the procedures

  4. A Proposal for Improving Administrative Preparation and Certification Programs.

    Science.gov (United States)

    Kritsonis, William A.

    Administration is the process of working with and through others to efficiently accomplish organizational goals by planning, organizing, leading, and controlling. The basic skills required by an effective administrator are technical, human, and conceptual. A strong school administrator preparation and certification program resembles the classical…

  5. A Proposal for Improving Administrative Preparation and Certification Programs.

    Science.gov (United States)

    Kritsonis, William A.

    Administration is the process of working with and through others to efficiently accomplish organizational goals by planning, organizing, leading, and controlling. The basic skills required by an effective administrator are technical, human, and conceptual. A strong school administrator preparation and certification program resembles the classical…

  6. Certification

    Science.gov (United States)

    Hayhurst, Kelly

    2010-01-01

    Objective 1: Provide regulators with a methodology for development of airworthiness requirements for certification of UAS. a) Rationale: a comprehensive methodology does not currently exist to support development of regulation for certification of UAS. Regulation is essential to enable routine access to the NAS. b) Approach: 1) assess existing approaches and classification schemes for deriving acceptable means of compliance to airworthiness requirements. 2) investigate a service-based approach to classification of UAS. 3) conduct comparative analysis of different methodologies. 4) work with FAA to determine best approach and conduct case study. 5) participate in regulatory/standards organizations developing safety and performance requirements for UAS. Objective 2: Provide regulators and industry with hazard and risk-related data to support criteria for UAS type design. a) Rationale: There is presently little UAS specific data (incident, accident, and reliability), especially in a civil context, to support risk assessment and development of standards and regulation. b) Approach: Identify gaps in existing data, provide measured data as needed, and formulate recommendations by: 1) evaluating UAS incident/accident data collection efforts and determining additional support necessary for regulation. 2) assessing UAS-specific hazards and risks. 3) evaluating need for reliability data for UAS-unique systems, components and subsystem, and determining additional measurement requirements. 4) developing guidance and best practices for UAS type design.

  7. Mathematics and Science Teachers' Preparation for National Board of Professional Teaching Standards Certification

    Science.gov (United States)

    Pyke, Curtis L.; Lynch, Sharon

    2005-01-01

    This paper reports on the results of a survey of mathematics and science candidates for National Board for Professional Teaching Standards (NBPTS) certification. The goal of the survey was to investigate the efficacy of collaboration and group support in preparation for NBPTS. The results of the survey describe the nature of supported preparation,…

  8. Game Theory: Trust Model for Common Criteria Certifications & Evaluations

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    Mohd Anuar Mat Isa

    2015-05-01

    Full Text Available International standard and certification play major role in product distributions and marketing activities. To be well accepted in global market, all IT products and services require international evaluation and certification such as Common Criteria (CC certification. This paper discusses some of the security, trust and privacy issues in Common Criteria that would happen during evaluation and certification of IT products and services. Our main intention is to help interested stake holders in choosing a finest authorizing member of CC certification for IT products and services using our new trust model. The proposed trust models takes into account the dynamically changing international relationship among nations which produces an index value during selection of finest CC authorizing member. The trust models use game theory to identify the finest CC authorizing member. We hope to contribute to this area of research by lessening the “cost to market” of IT products and related services. It is anticipated that it would give positive impact on global business transaction by having better and wider acceptability using our models in the selection of the finest CC authorizing member, CC consumer, and vendor (manufacturer.

  9. Evaluation of Ecological Criteria of Biofuel Certification in Germany

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    Kirsten Selbmann

    2016-09-01

    Full Text Available The share of biofuels has increased significantly over the last decade, which has lead to several negative impacts on the environment. As a solution, several governments worldwide have promoted the use of certification systems, which have been implemented and in some cases have even been established as mandatory regulations. Due to the focus of the public debate, standard-setting has mainly been limited to developing and newly industrializing countries. Hence, the issues of environmental impacts as a consequence of agricultural intensification in Germany has been given little attention, and the question whether existing biofuel certification systems sufficiently cover ecological issues remains. In order to answer this question, this study performs a benchmarking analysis of selected certification systems, whereby their ability to ensure ecological sustainability is evaluated and compared. The assessment reveals that the currently existing national ordinances, like Cross Compliance, are in many aspects insufficient to ensure sustainability. Contrarily, they often deter necessary discussions to tackle these issues.

  10. The Status of Program Evaluation Expectations in State School Counselor Certification Requirements.

    Science.gov (United States)

    Trevisan, Michael S.

    2000-01-01

    Studied state school counselor certification requirements with respect to program evaluation expectations. Responses of state certification offices show 19 states and the District of Columbia require some form of program evaluation knowledge and skills, but only Colorado and Washington require knowledge of the program evaluation standards…

  11. Construction of Performance Evaluation Index System for Enterprises Implementing Green Food Certification Based on BSC

    OpenAIRE

    Wu, Xiu-Min; Wang, Shu-han

    2010-01-01

    According to the investigation results of 70 green food enterprises in Sichuan Province, the strategic targets of enterprises implementing green food certification are discussed by borrowing ideas from the Balanced Score Card. Balanced Score Card index system is selected and improved according to the changes of enterprises before and after implementing the green food certification system. Then, the performance evaluation index system of enterprises implementing green food certification is est...

  12. Objective Evaluation in an Online Geographic Information System Certificate Program

    Directory of Open Access Journals (Sweden)

    Scott L. WALKER

    2005-01-01

    Full Text Available Objective Evaluation in an Online Geographic Information System Certificate Program Asst. Professor. Dr. Scott L. WALKER Texas State University-San Marcos San Marcos, Texas, USA ABSTRACT Departmental decisions regarding distance education programs can be subject to subjective decision-making processes influenced by external factors such as strong faculty opinions or pressure to increase student enrolment. This paper outlines an evaluation of a departmental distance-education program. The evaluation utilized several methods that strived to inject objectivity in evaluation and subsequent decision-making. A rapid multi-modal approach included evaluation methods of (1 considering the online psychosocial learning environment, (2 content analyses comparing the online version of classes to face-to-face versions, (3 cost comparisons in online vs. face-to-face classes, (4 student outcomes, (5 student retention, and (6 benchmarking. These approaches offer opportunities for departmental administrators and decision-making committees to make judgments informed by facts rather than being influenced by the emotions, beliefs, or opinions of organizational dynamics.

  13. Evaluating the Skills Strategy through a Graduate Certificate in Management: An Experiential Learning Theory Approach

    Science.gov (United States)

    Butler, Michael J. R.; Gheorghiu, Lidia

    2010-01-01

    Purpose: The purpose of this paper is to evaluate how a UK business school is addressing the Government's skills strategy through its Graduate Certificate in Management, and to identify good practice and development needs and to clarify how the Graduate Certificate is adapting to the needs of Generation X and Millennial students. The paper also…

  14. Evaluating the Skills Strategy through a Graduate Certificate in Management: An Experiential Learning Theory Approach

    Science.gov (United States)

    Butler, Michael J. R.; Gheorghiu, Lidia

    2010-01-01

    Purpose: The purpose of this paper is to evaluate how a UK business school is addressing the Government's skills strategy through its Graduate Certificate in Management, and to identify good practice and development needs and to clarify how the Graduate Certificate is adapting to the needs of Generation X and Millennial students. The paper also…

  15. Evaluating an educational intervention to improve the accuracy of death certification among trainees from various specialties

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    Villar Jesús

    2007-11-01

    Full Text Available Abstract Background The inaccuracy of death certification can lead to the misallocation of resources in health care programs and research. We evaluated the rate of errors in the completion of death certificates among medical residents from various specialties, before and after an educational intervention which was designed to improve the accuracy in the certification of the cause of death. Methods A 90-min seminar was delivered to seven mixed groups of medical trainees (n = 166 from several health care institutions in Spain. Physicians were asked to read and anonymously complete a same case-scenario of death certification before and after the seminar. We compared the rates of errors and the impact of the educational intervention before and after the seminar. Results A total of 332 death certificates (166 completed before and 166 completed after the intervention were audited. Death certificates were completed with errors by 71.1% of the physicians before the educational intervention. Following the seminar, the proportion of death certificates with errors decreased to 9% (p Conclusion Major errors in the completion of the correct cause of death on death certificates are common among medical residents. A simple educational intervention can dramatically improve the accuracy in the completion of death certificates by physicians.

  16. Preparation and certification of arsenobetaine reference material NMIJ CRM 7901-a.

    Science.gov (United States)

    Narukawa, Tomohiro; Chiba, Koichi; Kuroiwa, Takayoshi; Yarita, Takashi; Takatsu, Akiko

    2007-09-01

    An arsenobetaine [(CH(3))(3)As(+)CH(2)COO(-)] solution reference material, NMIJ CRM 7901-a, intended for use in the speciation of arsenic compounds, was developed and certified by the National Metrology Institute of Japan (NMIJ), part of the National Institute of Advanced Industrial Science and Technology (AIST). The high-purity arsenobetaine powder was synthesized from trimethylarsine [(CH(3))(3)As], and it was dissolved in water in order to prepare 20 mg kg(-1) of arsenobetaine standard solution. The solution was bottled in 500 bottles (each containing 10 ml). Certification of the CRM for arsenobetaine was conducted by NMIJ. The concentration of As was determined by four independent analytical techniques (ICP-MS, ICP-OES, GFAAS and LC-ICP-MS), and each result was converted to the arsenobetaine concentration by applying an appropriate factor. The arsenobetaine concentration in the CRM was thus certified.

  17. Comparative Analyses of the Teaching Methods and Evaluation Practices in English Subject at Secondary School Certificate (SSC) and General Certificate of Education (GCE O-Level) in Pakistan

    Science.gov (United States)

    Behlol, Malik Ghulam; Anwar, Mohammad

    2011-01-01

    The study was conducted to compare the teaching methods and evaluation practices in English subject at secondary school certificate (SSC) and general certificate of education GCE-O-level in Pakistan. The population of the study was students, teachers and experts at SSC and 0-level in the Punjab province. Purposive and random sampling techniques…

  18. Experimental evaluation of the certification-trail method

    Science.gov (United States)

    Sullivan, Gregory F.; Wilson, Dwight S.; Masson, Gerald M.; Itoh, Mamoru; Smith, Warren W.; Kay, Jonathan S.

    1993-01-01

    Certification trails are a recently introduced and promising approach to fault-detection and fault-tolerance. A comprehensive attempt to assess experimentally the performance and overall value of the method is reported. The method is applied to algorithms for the following problems: huffman tree, shortest path, minimum spanning tree, sorting, and convex hull. Our results reveal many cases in which an approach using certification-trails allows for significantly faster overall program execution time than a basic time redundancy-approach. Algorithms for the answer-validation problem for abstract data types were also examined. This kind of problem provides a basis for applying the certification-trail method to wide classes of algorithms. Answer-validation solutions for two types of priority queues were implemented and analyzed. In both cases, the algorithm which performs answer-validation is substantially faster than the original algorithm for computing the answer. Next, a probabilistic model and analysis which enables comparison between the certification-trail method and the time-redundancy approach were presented. The analysis reveals some substantial and sometimes surprising advantages for ther certification-trail method. Finally, the work our group performed on the design and implementation of fault injection testbeds for experimental analysis of the certification trail technique is discussed. This work employs two distinct methodologies, software fault injection (modification of instruction, data, and stack segments of programs on a Sun Sparcstation ELC and on an IBM 386 PC) and hardware fault injection (control, address, and data lines of a Motorola MC68000-based target system pulsed at logical zero/one values). Our results indicate the viability of the certification trail technique. It is also believed that the tools developed provide a solid base for additional exploration.

  19. Does Teacher Preparation Matter? Evidence about Teacher Certification, Teach for America, and Teacher Effectiveness.

    Directory of Open Access Journals (Sweden)

    Linda Darling-Hammond

    2005-10-01

    Full Text Available Recent debates about the utility of teacher education have raised questions about whether certified teachers are, in general, more effective than those who have not met the testing and training requirements for certification, and whether some candidates with strong liberal arts backgrounds might be at least as effective as teacher education graduates. This study examines these questions with a large student-level data set from Houston, Texas that links student characteristics and achievement with data about their teachers' certification status, experience, and degree levels from 1995-2002. The data set also allows an examination of whether Teach for America (TFA candidates-recruits from selective universities who receive a few weeks of training before they begin teaching-are as effective as similarly experienced certified teachers. In a series of regression analyses looking at 4th and 5th grade student achievement gains on six different reading and mathematics tests over a six-year period, we find that certified teachers consistently produce stronger student achievement gains than do uncertified teachers. These findings hold for TFA recruits as well as others. Controlling for teacher experience, degrees, and student characteristics, uncertified TFA recruits are less effective than certified teachers, and perform about as well as other uncertified teachers. TFA recruits who become certified after 2 or 3 years do about as well as other certified teachers in supporting student achievement gains; however, nearly all of them leave within three years. Teachers' effectiveness appears strongly related to the preparation they have received for teaching.

  20. Piloting an integrated education pathway as a strategy to prepare for and encourage oncology specialty certification.

    Science.gov (United States)

    Savage, Pamela; Fitzgerald, Barbara; Lee, Charlotte T

    2015-01-01

    Although continuing nursing education is crucial to improve professional and patient outcomes, programs in oncology nursing remain scarce, piecemeal, and focused on one modality of treatment, which limits the effectiveness of education interventions. The objectives of this paper are to describe the development and implementation of a longitudinal specialized oncology nursing education pathway program, and the evaluation results of a year-long pilot of the first stage of the program at a large university-affiliated cancer centre. Preliminary findings indicated that participants' perceived competence in health assessment and symptom management was improved after one year of enrolment in the education pathway. Next steps following this pilot, including implications for participants with regards to attaining oncology certification are also discussed.

  1. Preparation and certification of the Polish reference material Virginia Tobacco Leaves (CTA-VTL-2) for inorganic trace analysis including microanalysis

    Energy Technology Data Exchange (ETDEWEB)

    Dybczynski, R.; Polkowska-Motrenko, H.; Samczynski, Z.; Szopa, Z.

    1997-12-31

    A new Polish certified reference material Virginia Tobacco Leaves (CTA-VTL-2) for inorganic trace analysis including microanalysis has been prepared. Certification of the candidate reference material was based on the world-wide interlaboratory comparison in which 60 laboratories from 18 countries, participated using various analytical methods and techniques. Data evaluation performed by means of the new multifunctional software package -SSQC. Recommended values were assigned for 33 and `information` values for 10 elements, respectively. The validity of `certified` values was confirmed for several elements using `very accurate` methods developed in this Laboratory. (author). 47 refs, 28 figs, 12 tabs.

  2. An Empirical Evaluation of Automated Theorem Provers in Software Certification

    Science.gov (United States)

    Denney, Ewen; Fischer, Bernd; Schumann, Johann

    2004-01-01

    We describe a system for the automated certification of safety properties of NASA software. The system uses Hoare-style program verification technology to generate proof obligations which are then processed by an automated first-order theorem prover (ATP). We discuss the unique requirements this application places on the ATPs, focusing on automation, proof checking, and usability. For full automation, however, the obligations must be aggressively preprocessed and simplified, and we demonstrate how the individual simplification stages, which are implemented by rewriting, influence the ability of the ATPs to solve the proof tasks. Our results are based on 13 certification experiments that lead to more than 25,000 proof tasks which have each been attempted by Vampire, Spass, e-setheo, and Otter. The proofs found by Otter have been proof-checked by IVY.

  3. A Subgroup Analysis of Predictors to Certification Examination Success in Differing Principal Preparation Programs.

    Science.gov (United States)

    Wilmore, Elaine L.

    This study addresses the factors of Graduate Record Examination scores (GRE), race, gender, and undergraduate grade point average (GPA) as predictors of principal certification examination success at a large urban university. The university has three programs that lead to a masters degree and principal certification. The regular program consists…

  4. Laboratory evaluation of commercial interferon preparations

    Energy Technology Data Exchange (ETDEWEB)

    Schoub, B.D.; Lyons, S.F.; Crespi, M.; Chiu, M.N. (University of the Witwatersrand, Johannesburg (South Africa). Dept. of Virology); Lomnitzer, R. (University of the Witwatersrand, Johannesburg (South Africa). Dept. of Immunology)

    1983-01-08

    The antiviral, antiproliferative and natural killer-cell (NKC) stimulatory activities of four commercial therapeutic interferon preparations were assayed in a laboratory. The antiviral and antiproliferative activities of each preparation were relatively similar, but an unexpectedly high NKC stimulatory activity was found in one of them. In-house determination of antiviral activity and evaluation of the antiproliferative and NKC stimulation potential of interferon preparations are essential before rational clinical trials of this agent are carried out.

  5. Evaluation of the certificate in emerging infectious disease research and the certificate in one health training programs, University of Florida.

    Science.gov (United States)

    Valentine, Marissa A; Perdue, Christopher L; Cummings, James F; Smith, Jacqueline C; Gray, Gregory C

    2015-03-01

    In developing countries, public health professionals and scientists need targeted training and practical skills to respond to global emerging infectious disease threats. The Certificate in Emerging Infectious Disease Research was developed in 2008 to aid such professionals to respond to complex emerging disease problems. The short-course was modified slightly in 2013 and renamed the Certificate in One Health. To evaluate the immediate impact of the short-course, an online survey of 176 past participants from both the courses was conducted. The survey tool assessed the program's process, impact, and outcome measures respectively via assessing the courses' perceived strengths and weaknesses, perceived skills gained, and the participants' current position, publication status, funding status, and educational attainment; 85 (48.3%) participants completed the survey. Reported program strengths included the curriculum, expertise of lecturers, and diversity of the training cohort. The principal reported weakness was the compressed academic schedule. The most frequently reported benefits included: epidemiological and biostatistical skills, followed by One-Health knowledge, and research skills. Twenty-eight percent of the survey respondents reported publishing one or more manuscripts since completing the course and 21% reported receiving research funding. The course appears to have had a positive, immediate impact on the students' self-perceived knowledge and capabilities. Copyright © 2014 Ministry of Health, Saudi Arabia. All rights reserved.

  6. Alcatel-Lucent Network Routing Specialist II (NRS II) Self-Study Guide Preparing for the NRS II Certification Exams

    CERN Document Server

    Warnock, Glenn

    2011-01-01

    The definitive resource for the NRS II exams—three complete courses in a book Alcatel-Lucent is a world leader in designing and developing scalable systems for service providers. If you are a network designer or operator who uses Alcatel-Lucent's 7750 family of service routers, prepare for certification as an A-L network routing specialist with this complete self-study course. You'll get thorough preparation for the NRS II exams while you learn to build state-of-the-art, scalable IP/MPLS-based service networks. The book provides you with an in-depth understanding of the protocols and tec

  7. Preparation and certification of Re-Os dating reference materials: Molybdenites HLP and JDC

    Science.gov (United States)

    Du, A.; Wu, S.; Sun, D.; Wang, Shaoming; Qu, W.; Markey, R.; Stain, H.; Morgan, J.; Malinovskiy, D.

    2004-01-01

    Two Re-Os dating reference material molybdenites were prepared. Molybdenite JDC and molybdenite HLP are from a carbonate vein-type molybdenum-(lead)- uranium deposit in the Jinduicheng-Huanglongpu area of Shaanxi province, China. The samples proved to be homogeneous, based on the coefficient of variation of analytical results and an analysis of variance test. The sampling weight was 0.1 g for JDC and 0.025 g for HLP. An isotope dilution method was used for the determination of Re and Os. Sample decomposition and preconcentration of Re and Os prior to measurement were accomplished using a variety of methods: acid digestion, alkali fusion, ion exchange and solvent extraction. Negative thermal ionisation mass spectrometry and inductively coupled plasma-mass spectrometry were used for the determination of Re and 187Os concentration and isotope ratios. The certified values include the contents of Re and Os and the model ages. For HLP, the Re content was 283.8 ?? 6.2 ??g g-1, 187Os was 659 ?? 14 ng g-1 and the Re-Os model age was 221.4 ?? 5.6 Ma. For JDC, the Re content was 17.39 ?? 0.32 ng g-1, 187Os was 25.46 ?? 0.60 ng g-1 and the Re-Os model age was 139.6 ?? 3.8 Ma. Uncertainties for both certified reference materials are stated at the 95% level of confidence. Three laboratories (from three countries: P.R. China, USA, Sweden) joined in the certification programme. These certified reference materials are primarily useful for Re-Os dating of molybdenite, sulfides, black shale, etc.

  8. Evaluation of virtual simulation in a master's-level nurse education certificate program.

    Science.gov (United States)

    Foronda, Cynthia; Lippincott, Christine; Gattamorta, Karina

    2014-11-01

    Master's-level, nurse education certificate students performed virtual clinical simulations as a portion of their clinical practicum. Virtual clinical simulation is an innovative pedagogy using avatars in Web-based platforms to provide simulated clinical experiences. The purpose of this mixed-methods study was to evaluate nurse educator students' experience with virtual simulation and the effect of virtual simulation on confidence in teaching ability. Aggregated quantitative results yielded no significant change in confidence in teaching ability. Individually, some students indicated change of either increased or decreased confidence, whereas others exhibited no change in confidence after engaging in virtual simulation. Qualitative findings revealed a process of precursors of anxiety and frustration with technical difficulties followed by outcomes of appreciation and learning. Instructor support was a mediating factor to decrease anxiety and technical difficulties. This study served as a starting point regarding the application of a virtual world to teach the art of instruction. As the movement toward online education continues, educators should further explore use of virtual simulation to prepare nurse educators.

  9. Teachers' Perceptions of National Board Certification as Professional Development and Evaluation

    Science.gov (United States)

    Tingle, Scot M.

    2014-01-01

    The purpose of the study was to investigate and describe the perceptions of recently NBPTS certified teachers in Maryland of the NBPTS certification process as both a professional development activity and as an evaluation instrument to identify teaching excellence. This descriptive survey used an online survey to collect data from 125 teachers for…

  10. Career and Technical Education Administration: Requirements, Certification/Licensure, and Preparation

    Science.gov (United States)

    Zirkle, Christopher J.; Jeffery, Jeremy O.

    2017-01-01

    The current climate of career and technical administration requirements in all 50 states was detailed and explored. An increasing number of states are not requiring specific career-technical administration certification/licensure in order to oversee secondary career and technical education (CTE) programs, with more states moving towards a general…

  11. Online learning communities can provide support for nurses preparing for certification examinations.

    Science.gov (United States)

    Pethtel, Pam

    2005-01-01

    Achieving certification is a benefit to the nurses, to their patients, and to the organizations that support them. Developing an online learning community is a simple way for the institution to offer support to the nursing staff. Providing the resources for creation of online learning communities demonstrates the facility's commitment to communication, education, and professional development.

  12. An Exploration of Virtual Study Groups Used to Prepare Candidates for a Professional Certification Exam

    Science.gov (United States)

    Kayne Chaplock, Sharon

    2011-01-01

    Professional credentials earned through certification programs are becoming an important way to demonstrate competency within a given discipline. With the globalization of business enterprises and associations, these credentials are eagerly sought by people located throughout the world. Candidates for these credentials often study together using…

  13. Preparation and certification of arsenate [As(V)] reference material, NMIJ CRM 7912-a.

    Science.gov (United States)

    Narukawa, Tomohiro; Kuroiwa, Takayoshi; Narushima, Izumi; Jimbo, Yasujiro; Suzuki, Toshihiro; Chiba, Koichi

    2010-05-01

    Arsenate [As(V)] solution reference material, National Metrology Institute of Japan (NMIJ) certified reference material (CRM) 7912-a, for speciation of arsenic species was developed and certified by NMIJ, the National Institute of Advanced Industrial Science and Technology. High-purity As(2)O(3) reagent powder was dissolved in 0.8 M HNO(3) solution and As(III) was oxidized to As(V) with HNO(3) to prepare 100 mg kg(-1) of As(V) candidate CRM solution. The solution was bottled in 400 bottles (50 mL each). The concentration of As(V) was determined by four independent analytical techniques-inductively coupled plasma mass spectrometry, inductively coupled plasma optical emission spectrometry, graphite furnace atomic absorption spectrometry, and liquid chromatography inductively coupled plasma mass spectrometry-according to As(V) calibration solutions, which were prepared from the arsenic standard of the Japan Calibration Service system and whose species was guaranteed to be As(V) by NMIJ. The uncertainties of all the measurements and preparation procedures were evaluated. The certified value of As(V) in the CRM is (99.53 +/- 1.67) mg kg(-1) (k = 2).

  14. Preparing Teachers to Teach Children with Special Educational Needs and Disabilities: The Significance of a National PGCE Development and Evaluation Project for Inclusive Teacher Education

    Science.gov (United States)

    Norwich, Brahm; Nash, Tricia

    2011-01-01

    This paper discusses issues arising from a national development and evaluation project, which (1) surveyed national primary and secondary postgraduate certificate programmes (PGCE) about how they prepare trainees to teach pupils with special educational needs; and (2) conducted a trial of a practical teaching task for preparing primary and…

  15. The Evaluation, Certification and Approbation of Oil and Gas Reserves in Mexico; Facts and Performance

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    Rodríguez-Padilla Víctor

    2013-06-01

    Full Text Available The evaluation, certification, approval and disclosure system of reserves applied in Mexico since 2010 aims to replicate the best practices of the international oil industry. It has gained rigor, simplicity, truthfulness and transparency, however its performance has been unequal: it has achieved to provide more certitude over the proved reserves but it has failed to dissipate the uncertainty over the probable and possible reserves.

  16. Orodispersible tablets of Lamotrigine: preparation and evaluation

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    Anand Patel

    2014-08-01

    Full Text Available The aim of the present research was to prepare orodispersible tablets of Lamotrigine for applications in epilepsy and convulsion related problems. Fast action onset is highly desirable in the control of this type of disease condition. All tablets were prepared by solid dispersion method using mannitol and synthetic superdisintegrants like Explotab, Cross Povidone and Micro Crystalline Cellulose. The different powder blends were evaluated for pre-formulation parameters. Effect of superdisintegrants on wetting, disintegration and dissolution parameters was studied. The tablets were evaluated for various parameters like wetting time, hardness, friability, drug content, disintegration and in vitro dissolution. Tablets with Explotab have shown good disintegrating features, also the dispersion not showing any bitter taste; indicate the capability of Vanilla used, as taste masking agents. Almost more than 99% of drug was released from the formulation(F4 within 27 min. Tablets have shown no appreciable changes with respect to physical appearance, drug content, disintegration time, and dissolution profiles.

  17. Construction of Performance Evaluation Index System for Enterprises Implementing Green Food Certification Based on BSC

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    According to the investigation results of 70 green food enterprises in Sichuan Province,the strategic targets of enterprises implementing green food certification are discussed by borrowing ideas from the Balanced Score Card.Balanced Score Card index system is selected and improved according to the changes of enterprises before and after implementing the green food certification system.Then,the performance evaluation index system of enterprises implementing green food certification is established.This index system has five strategic directions of finance,customer,internal management,social ecology,and learning and growth,and has 11 strategic targets,such as increasing operating profit,expanding market,improving the quality of staff,improving the production technology level,enhancing the core competence of enterprises,ensuring food safety,improving the physical quality of human,and protecting ecological environment.According to the principles of guidance,scientificity,comparability,feasibility,and significance,a total of 24 evaluation indices are established,including the change of product cost,change of product sales revenue,change of profit rate,change of return on investment,change of internal and external lost cost,change of the proportion of certified products in total sales and so on.

  18. Preparation and certification of the Polish reference material `Oriental Tobacco Leaves` (CTA-OTL-1) for inorganic trace analysis

    Energy Technology Data Exchange (ETDEWEB)

    Dybczynski, R.; Polkowska-Motrenko, H.; Samczynski, Z.; Szopa, Z. [Institute of Nuclear Chemistry and Technology, Warsaw (Poland)

    1996-12-31

    A new Polish certified reference material `Oriental Tobacco Leaves` (CTA-OTL-1) for inorganic trace analysis was prepared. Fresh tobacco leaves of variety `Oriental` were dried, ground and sieved. All precautions were taken to avoid contamination of material with metals. The next step was homogenization. Preliminary homogeneity checking consisted in determining of Ca, Fe and K content by X-ray fluorescence. Final homogeneity testing was performed by neutron activation analysis determining Co, Cr, Fe and Rb. To assure long-term stability, the whole lot of material was sterilized by electron beam radiation. Certification of the candidate reference material was done on the basis of world-wide interlaboratory comparisons exercise in which 61 laboratories, using various analytical methods participated. (author). 30 refs, 12 tabs, 21 figs.

  19. Preparing and evaluating delivery systems for proteins

    DEFF Research Database (Denmark)

    Jorgensen, L; Moeller, E H; van de Weert, M

    2006-01-01

    From a formulation perspective proteins are complex and therefore challenging molecules to develop drug delivery systems for. The success of a formulation depends on the ability of the protein to maintain the native structure and activity during preparation and delivery as well as during shipping...... and long-term storage of the formulation. Therefore, the development and evaluation of successful and promising drug delivery systems is essential. In the present review, some of the particulate drug delivery systems for parenteral delivery of protein are presented and discussed. The challenge...... for incorporation of protein in particulate delivery systems is exemplified by water-in-oil emulsions....

  20. Evaluation and certification of heater assemblies developed for thermal vacuum acceptance testing

    Science.gov (United States)

    Allen, J. E.

    1986-01-01

    Preparation of Rockwell International's Thermal Vacuum Chamber for acceptance testing of a mass produced satellite required the development of unique quartz lamp and hot wire heater assemblies. Testing performed on the basic elements of these heaters is described, as is the final testing done to certify that the heater assemblies meet the thermal requirements for acceptance testing. The methods and procedures of thermal mapping used during the development and final certification of these heater assemblies are presented. The absence of a definitive standard for determining flux distribution and heating boundaries for heaters of this type required the development of a test plan incorporating several thermal mapping techniques. These techniques include the development of heat flux using a multiple calorimeter array for both vacuum and ambient test conditions, and a photographic method for detecting heating boundaries. The test plan and thermal mapping techniques are discussed.

  1. Preparation, certification and interlaboratory analysis of workplace air filters spiked with high-fired beryllium oxide.

    Science.gov (United States)

    Oatts, Thomas J; Hicks, Cheryl E; Adams, Amy R; Brisson, Michael J; Youmans-McDonald, Linda D; Hoover, Mark D; Ashley, Kevin

    2012-02-01

    Occupational sampling and analysis for multiple elements is generally approached using various approved methods from authoritative government sources such as the National Institute for Occupational Safety and Health (NIOSH), the Occupational Safety and Health Administration (OSHA) and the Environmental Protection Agency (EPA), as well as consensus standards bodies such as ASTM International. The constituents of a sample can exist as unidentified compounds requiring sample preparation to be chosen appropriately, as in the case of beryllium in the form of beryllium oxide (BeO). An interlaboratory study was performed to collect analytical data from volunteer laboratories to examine the effectiveness of methods currently in use for preparation and analysis of samples containing calcined BeO powder. NIST SRM(®) 1877 high-fired BeO powder (1100 to 1200 °C calcining temperature; count median primary particle diameter 0.12 μm) was used to spike air filter media as a representative form of beryllium particulate matter present in workplace sampling that is known to be resistant to dissolution. The BeO powder standard reference material was gravimetrically prepared in a suspension and deposited onto 37 mm mixed cellulose ester air filters at five different levels between 0.5 μg and 25 μg of Be (as BeO). Sample sets consisting of five BeO-spiked filters (in duplicate) and two blank filters, for a total of twelve unique air filter samples per set, were submitted as blind samples to each of 27 participating laboratories. Participants were instructed to follow their current process for sample preparation and utilize their normal analytical methods for processing samples containing substances of this nature. Laboratories using more than one sample preparation and analysis method were provided with more than one sample set. Results from 34 data sets ultimately received from the 27 volunteer laboratories were subjected to applicable statistical analyses. The observed

  2. Evaluation of the U.S. Department of Energy Challenge Home Program Certification of Production Builders

    Energy Technology Data Exchange (ETDEWEB)

    Kerrigan, P. [Building Science Corporation, Somerville, MA (United States); Loomis, H. [Building Science Corporation, Somerville, MA (United States)

    2014-09-01

    The purpose of this project was to evaluate integrated packages of advanced measures in individual test homes to assess their performance with respect to Building America program goals, specifically compliance with the DOE Challenge Home Program. BSC consulted on the construction of five test houses by three cold climate production builders in three U.S. cities and worked with the builders to develop a design package tailored to the cost-related impacts for each builder. Also, BSC provided support through performance testing of the five test homes. Overall, the builders have concluded that the energy related upgrades (either through the prescriptive or performance path) represent reasonable upgrades. The builders commented that while not every improvement in specification was cost effective (as in a reasonable payback period), many were improvements that could improve the marketability of the homes and serve to attract more energy efficiency discerning prospective homeowners. However, the builders did express reservations on the associated checklists and added certifications. An increase in administrative time was observed with all builders. The checklists and certifications also inherently increase cost due to: adding services to the scope of work for various trades, such as HERS Rater, HVAC contractor; and increased material costs related to the checklists, especially the EPA Indoor airPLUS and EPA WaterSense® Efficient Hot Water Distribution requirement.

  3. Evaluation of the U.S. Department of Energy Challenge Home Program Certification of Production Builders

    Energy Technology Data Exchange (ETDEWEB)

    Kerrigan, P.; Loomis, H.

    2014-09-01

    The purpose of this project was to evaluate integrated packages of advanced measures in individual test homes to assess their performance with respect to Building America Program goals, specifically compliance with the DOE Challenge Home Program. BSC consulted on the construction of five test houses by three Cold Climate production builders in three separate US cities. BSC worked with the builders to develop a design package tailored to the cost-related impacts for each builder. Therefore, the resulting design packages do vary from builder to builder. BSC provided support through this research project on the design, construction and performance testing of the five test homes. Overall, the builders have concluded that the energy related upgrades (either through the prescriptive or performance path) represent reasonable upgrades. The builders commented that while not every improvement in specification was cost effective (as in a reasonable payback period), many were improvements that could improve the marketability of the homes and serve to attract more energy efficiency discerning prospective homeowners. However, the builders did express reservations on the associated checklists and added certifications. An increase in administrative time was observed with all builders. The checklists and certifications also inherently increase cost due to: 1. Adding services to the scope of work for various trades, such as HERS Rater, HVAC contractor; 2. Increased material costs related to the checklists, especially the EPA Indoor airPLUS and EPA WaterSense(R) Efficient Hot Water Distribution requirement.

  4. Preparation and evaluation of proliposomes containing clotrimazole.

    Science.gov (United States)

    Ning, Mei-Ying; Guo, Ying-Zhi; Pan, Huai-Zhong; Yu, He-Ming; Gu, Zhong-Wei

    2005-06-01

    Clotrimazole (CT)-containing proliposomes were prepared by penetrating an ethanol solution of CT and Egg phosphatidylcholine (PC) into microporous sorbitol particles, followed by vacuum evaporation of the solvent. As a result, CT proliposomes with free-flowing flowability were obtained. On contact with water, the proliposomes were rapidly converted into a liposomal dispersion, in which a certain amount of CT was entrapped by the liposomes. The result in scanning electronic micrograph confirmed the formation of liposomes structures from proliposomes, and the particles revealed round or ellipse. The ratio of drug to total lipid, ratio of PC to cholesterol and ratio of lipid to sorbitol affected the entrapment efficiency (EE%). The EE% of optimized formulation (CT 10 mg, 0.1 g total lipid, PC/CH ratio is 60 : 40 and 1 g sorbitol) in this investigation was 96.2+/-1.5%. The proliposomes system can provide sustaining release in simulated vaginal fluid at 37+/-1 degrees C for 24 h. In-vivo performance of blank proliposomes, a physical mixture of sorbitol and drug, clotrimazole proliposomes and commercial ointment formulation were evaluated using antifungal activity test. At 7 d post-dose, the c.f.u. of C. albicans decreased in proliposomes-treated groups than ointment and the physical mixture (t-Student, pcandidasis with reduced dosing interval.

  5. Environmental certification: a scientific tool for sustainability. Evaluation of possible indicators for the environmental performance evaluation (EPE) of Ravenna province (Italy).

    Science.gov (United States)

    Panzieri, Margherita; Marchettini, Nadia; Ridolfi, Roberto

    2003-04-01

    Environmental certification is becoming the main tool for application of sustainable development principles. The European Regulation Emas and the international standard ISO 14001 both require for certification, to perform an environmental management system to prevent environmental impacts and to continuously improve environmental performance. For a good environmental performance evaluation (EPE), certification needs to use scientific methodologies and to interface with scientific research; here we proposed emergy analysis as a valid method for EPE and emergetic environmental performance and condition indicators (EPIs, ECIs) to monitor a territorial system: Ravenna province (Italy). Together with emergy indicators were selected other indicators for a deeper EPE: emitted/adsorbed CO2, energy consumptions, air and water pollution measures. The paper showed that Ravenna system has a good environmental performance and demonstrated how different indicators from the most advanced chemical research (chemical-physical, analytical, etc.) contribute to a complete EPE of a complex territorial system and are useful for environmental certification and sustainable development.

  6. Evaluation of American Board of Orthodontics certification protocols in postgraduate orthodontic programs in the United States and Canada.

    Science.gov (United States)

    Park, Jae Hyun; Putrus, Raphael R; Pruzansky, Dawn P; Grubb, John

    2017-03-01

    The objective of this study was to identify the board certification protocols that hospital and university-based postgraduate orthodontic programs have in place to prepare residents for the American Board of Orthodontics (ABO) certification examination. An electronic survey was sent to the program directors of each of the 72 postgraduate orthodontic programs in the United States and Canada. The survey consisted of 49 questions about demographics, resident case assignment protocols, and ABO examination preparation methods. The response rate was 81%. Most programs were 30 to 36 months in length (72.7%). Many residents had a case load of 51 to 75 during their first year (50.9%), with an average maximum case load of 70 to 109. There was a positive correlation with both the number of cases that first-year residents start and the length of the program (Spearman correlation coefficient = 0.379; P clinical mock board examination. ABO cases are identified within the first 6 months of most programs. About 88% of respondents believe that residents take advantage of the banking system, and that over the past 5 years ABO Initial Certification Examination applications have increased. Most program directors (89.1%) believe that their program length is sufficient for board preparation. Subjects tested in the written examination are integrated into the didactic curriculum and strengthened with ongoing literature reviews, with a passing rate over 90%. Clinical examination preparation varies, with most programs requiring a mock board examination for graduation. Total participation in both the Initial Certification Examination and banking has increased since 2010; better follow-up protocols are needed to track residents after graduation. Copyright © 2017 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.

  7. Preparation, characterization and certification of uranium isotope reference materials; Preparacao, Caracterizacao e certificacao de materiais de referencia isotopicos de uranio

    Energy Technology Data Exchange (ETDEWEB)

    Oliveira Junior, Olivio Pereira de

    2006-07-01

    This work describes the preparation, characterization and certification of a set of uranium isotope reference materials ranging from 0.5 to 20.0 % of {sup 235}U in mass. The most important concepts of metrology in chemical measurements were applied so that the certified quantities in these materials could be directly traceable to the International System of Units (SI). As a consequence of this approach, these materials can be used in the instruments calibration, estimation of measurement uncertainty, method validation, assessment of performance of analysts, quality control routines and interlaboratory comparison programmes. The most advanced methods and techniques in mass spectrometry, that is, gas source mass spectrometry (GSMS), thermal ionisation mass spectrometry (TIMS) and inductively coupled plasma mass spectrometry (ICPMS) were investigated to identify which are the dominant components in the uncertainty and to quantify its contribution to the final value of the measurement uncertainty of the isotopic ratio. The results obtained were then compared to verify which are the methods and techniques associated to the lowest measurement uncertainty values. The isotope amount ratio n({sup 235}U)/n({sup 238}U) was certified in the materials produced to expanded uncertainties ranging from 0.02 to 0.10 % and the ratios n({sup 234}U)/n({sup 238}U) and n({sup 236}U)/n({sup 238}U), to uncertainties ranging from 0.03 to 2.20 %. These values fully comply to the requirements of the isotopic characterization of nuclear fuel as well as the analysis of environmental samples for nuclear safeguards. (author)

  8. Preparation and evaluation of sulphonamide nonionic surfactants

    Energy Technology Data Exchange (ETDEWEB)

    Ahmed, M. H. M.

    2010-07-01

    Alkyl (octyl, decyl and dodecyl; C{sub 8}, C{sub 1}0 and C{sub 1}2) benzene sulphonyl chloride was used in the preparation of a novel series of nonionic surfactants (IV-VI)a-c, (VII-IX) a-c and (X-XII)a-c. The preparations were completed by reacting each alkyl (C{sub 8}, C{sub 1}0 and C{sub 1}2) benzene sulphonyl chloride with ethanolamine to give (I-III) respectively. The resulting products were reacted separately with ethylene oxide in the presence of different (base KOH, Lewis acid SnCl4 and k10 clay) catalysts to produce different moles of nonionic surfactants (5, 7 and 9) in sequence corresponding to (IV-VI)a-c, (VII-IX) a-c and (X-XII)a-c respectively. The chemical structures of prepared nonionic surfactants were elucidated by IR and 1HNMR spectra. The surface activity, biodegradability and biological activities of the prepared compounds were investigated. The obtained data show that these compounds have good surface and biological activities as well as reasonable biodegradability properties. (Author) 30 refs.

  9. Taiwan Teacher Preparation Program Evaluation: Some Critical Perspectives

    Science.gov (United States)

    Liu, Tze-Chang

    2015-01-01

    This paper focuses on the influences and changes of recent Taiwan teacher preparation program evaluation (TTPPE) as one of the national evaluation projects conducted by the Higher Education Evaluation and Accreditation Council of Taiwan. The main concerns are what kind of ideology is transformed through the policy by means of evaluation, and what…

  10. Criteria for the evaluation and certification of long-term digital archives in the earth sciences

    Science.gov (United States)

    Klump, Jens

    2010-05-01

    Digital information has become an indispensable part of our cultural and scientific heritage. Scientific findings, historical documents and cultural achievements are to a rapidly increasing extent being presented in electronic form - in many cases exclusively so. However, besides the invaluable advantages offered by this form, it also carries a serious disadvantage: users need to invest a great deal of technical effort in accessing the information. Also, the underlying technology is still undergoing further development at an exceptionally fast pace. The rapid obsolescence of the technology required to read the information combined with the frequently imperceptible physical decay of the media themselves represents a serious threat to preservation of the information content. Many data sets in earth science research are from observations that cannot be repeated. This makes these digital assets particularly valuable. Therefore, these data should be kept and made available for re-use long after the end of the project from which they originated. Since research projects only run for a relatively short period of time, it is advisable to shift the burden of responsibility for long-term data curation from the individual researcher to a trusted data repository or archive. But what makes a trusted data repository? Each trusted digital repository has its own targets and specifications. The trustworthiness of digital repositories can be tested and assessed on the basis of a criteria catalogue. This is the main focus of the work of the nestor working group "Trusted repositories - Certification". It identifies criteria which permit the trustworthiness of a digital repository to be evaluated, both at the organisational and technical levels. The criteria are defined in close collaboration with a wide range of different memory organisations, producers of information, experts and other interested parties. This open approach ensures a high degree of universal validity, suitability for

  11. Wind turbine certification - the committee draft by IEC-TC88-WG9

    Energy Technology Data Exchange (ETDEWEB)

    Hauge Madsen, P. [Risoe National Lab., Wind Energy and Atmospheric Physics Dept., Roskilde (Denmark)

    1999-03-01

    In 1995 the Technical Committee TC88 of the International Electrotechnical Commission decided to start a new work item, namely to prepare a standard for the certification procedures for wind turbines with respect to safety, performance, interaction with the public grid, environmental requirements and the documentation requested by the certification bodies. The purpose was to provide a common basis for certification of wind turbines, including a basis for accreditation of certification bodies and mutual recognition of certificates. A working group (WG9) was created to prepare a committee draft. WG9 submitted the result late in 1998 to TC88. This paper presents the committee draft Standard, which defines a certification system for Wind Turbine Generator Systems. Key issues in the evaluation of conformity with these standards and identified needs for other technical criteria and procedures are presented. (au) 10 refs.

  12. Preparation and evaluation of sulphonamide nonionic surfactants

    Directory of Open Access Journals (Sweden)

    Ahmed, M. H. M.

    2010-03-01

    Full Text Available Alkyl (octyl, decyl and dodecyl; C8,C10 and C12 benzene sulphonyl chloride was used in the preparation of a novel series of nonionic surfactants (IV-VIa-c, (VII-IX a-c and (X-XIIa-c. The preparations were completed by reacting each alkyl (C8,C10 and C12 benzene sulphonyl chloride with ethanolamine to give (I-III respectively. The resulting products were reacted separately with ethylene oxide in the presence of different (base KOH, Lewis acid SnCl4 and k10 clay catalysts to produce different moles of nonionic surfactants (5, 7 and 9 in sequence corresponding to (IV-VIa-c, (VII-IX a-c and (X-XIIa-c respectively. The chemical structures of prepared nonionic surfactants were elucidated by IR and 1HNMR spectra. The surface activity, biodegradability and biological activities of the prepared compounds were investigated. The obtained data show that these compounds have good surface and biological activities as well as reasonable biodegradability properties.Cloruros de sulfonilbenceno alquilados (octil, decil, dodecil; C8, C10 y C12 fueron usados en la preparación de una nueva serie de surfactantes no iónicos (IV-VIa-c, (VII-IX a-c and (X-XIIa-c. Las preparaciones fueron completadas por reacción de cada cloruro de sulfonilbenceno alquilado (C8, C10 y C12 con etanolamina para dar los compuestos (IIII, respectivamente. Los anteriores productos reaccionaron separadamente con óxido de etileno en presencia de diferentes catalizadores (la base KOH, el ácido de Lewis SnCl4 y la arcilla k10 para producir secuencialmente diferentes surfactantes no iónicos con distintos moles de óxido de etileno (5, 7 y 9: (IV-VIa-c, (VII-IXa-c and (X-XIIa-c, respectivamente. La estructura química de los surfactantes no iónicos preparados fueron elucidadas mediante sus espectros de IR y 1H RMN. Las propiedades tensoactivas, biodegradabilidad y actividad biológica de los compuestos preparados fueron investigados. Los datos obtenidos muestran que estos compuestos tienen

  13. Evaluation of torque within manual preparation with root canal instruments

    OpenAIRE

    Gorski, Christof

    2016-01-01

    Used root canal instruments are often deformed; they can fracture, persist and reinfect teeth. There are no evident studies consulting torque and manual preparation of root canals. Thus, the purpose of this study is the evaluation of torque within manual preparation in connection with its impact on file deformation. With the aid of torque measurement a change in dental education could potentially be achieved.

  14. Preparation and evaluation of antisera to oxytocin

    Energy Technology Data Exchange (ETDEWEB)

    Fingerova, H.; Slaninova, J. (Palackeho Univ., Olomouc (Czechoslovakia). Lekarska Fakulta); Janouskova, M.; Talas, M. (Ceskoslovenska Akademie Ved, Prague. Ustav Organicke Chemie a Biochemie)

    1981-01-01

    In co-operation with the Institute of Organic Chemistry and Biochemistry of the Czechoslovak Academy of Sciences in Prague we attempted to prepare a specific oxytocin antibody. 9 rabbits were immunized using an oxytocin and thyreoglobulin conjugate in two dosages. Only two rabbits responded by sufficient antibody production to the first immunization by intradermal injections. The second immunization was only performed in the said two rabbits, thus obtaining higher titer antisera. In preliminary experiments, in the first sampling in rabbit No.8 cross-reaction was tested with 8-arginine-vasopressin. It was found to be negligible. The standard oxytocin curve was found sensitive within 8 to 1000 pg/tube. The experiments carried out so far show good prospects of introducing sensitive and specific radioimmunological determination of oxytocin using noncommercial substances.

  15. Preparation and evaluation of cilnidipine microemulsion

    Directory of Open Access Journals (Sweden)

    Hemal Tandel

    2012-01-01

    Full Text Available Cilnidipine, a calcium channel blocker having neuroprotective action and BCS Class II drug, hence formulating in Microemulsion will increase solubility, absorption and bioavailability. The formulation was prepared using titration method by tocotrienol, tween 20 and transcutol HP as oil, surfactant and co-surfactant and characterized for dilutability, dye solubility, assay (98.39±0.06, pH (6.6±1.5, Viscosity (98±1.0 cps and Conductivity (0.2±0.09 μS/cm. The formulation was optimized on basis of percentage transmittance (99.269±0.23 at 700 nm, Globule size (13.31±4.3 nm and zeta potential (-11.4±2.3 mV. Cilnidipine microemulsion was found to be stable for 3 months.

  16. Preparation and In Vitro, In Vivo Evaluation of Clarithromycin Microcapsules

    OpenAIRE

    Hu, LianDong; Liu, Wei; Li, Li; Zhao, Jiqiang; Yang, Xun

    2011-01-01

    PURPOSE: To develop and validate a method to prepare clarithromycin (CLM) microcapsules to mask the bitter taste and provide effective treatment, and evaluate the quality of microcapsules in detail, especially the in vitro and in vivo pharmacokinetics behavior. METHODS: CLM microcapsules were prepared using ethyl cellulose as matrix material by an emulsion solvent diffusion method. The physicochemical property, in vitro release study, sensory test and stability test were evaluated. Self-made ...

  17. Preparation and evaluation of orthodontic setup

    OpenAIRE

    Telma Martins de Araújo; Lílian Martins Fonseca; Luciana Duarte Caldas; Roberto Amarante Costa-Pinto

    2012-01-01

    INTRODUCTION: An orthodontic or diagnostic setup consists in cutting and realigning the teeth in plaster models, making it an important resource in orthodontic treatment planning. OBJECTIVE: The aim of this article is to provide a detailed description of a technique to build an orthodontic setup model and a method to evaluate it. CONCLUSIONS: Although laborious, orthodontic setup procedure and analysis can provide important information such as the need for dental extractions, interproximal st...

  18. Preparation and evaluation of orthodontic setup

    Directory of Open Access Journals (Sweden)

    Telma Martins de Araújo

    2012-06-01

    Full Text Available INTRODUCTION: An orthodontic or diagnostic setup consists in cutting and realigning the teeth in plaster models, making it an important resource in orthodontic treatment planning. OBJECTIVE: The aim of this article is to provide a detailed description of a technique to build an orthodontic setup model and a method to evaluate it. CONCLUSIONS: Although laborious, orthodontic setup procedure and analysis can provide important information such as the need for dental extractions, interproximal stripping, anchorage system, among others.

  19. Preparation and evaluation of orthodontic setup

    OpenAIRE

    Araújo, Telma Martins de; Fonseca, Lílian Martins; Caldas, Luciana Duarte; Pinto, Roberto Amarante Costa

    2012-01-01

    p. 146-165 Introduction: An orthodontic or diagnostic setup consists in cutting and realigning the teeth in plaster models, making it an important resource in orthodontic treatment planning. Objetive: The aim of this article is to provide a detailed description of a technique to build an orthodontic setup model and a method to evaluate it. Conclusions: Although laborious, orthodontic setup procedure and analysis can provide important information such as the need for dental extractions, ...

  20. Identifying, Preparing and Evaluating Army Instructors

    Science.gov (United States)

    2016-04-01

    virtual reality surgical simulator. IEEE Transactions on Information Technology in Biomedicine, 10(1), 51-58. Haney, A. (1997). The role of... teaching , training and instructing as opposed to methods and principles of learning. Methods of learning include reading, observing and other forms of...specific course content (K1), general teaching and evaluation strategies and methods (K4, K5, K6, K8), learner characteristics (K2, K3), and

  1. Meeting the Pepsi Challenge: Preparing Evaluation Programs for Scholastic Improvement.

    Science.gov (United States)

    Hopkins, Mona S.

    This document provides guidelines for preparing evaluation programs for scholastic improvement. The philosophy underlying assessment and accountability is discussed, with specific reference to the positive and negative aspects of assessment. The design of a curriculum evaluation model is presented, including goal identification, data gathering,…

  2. Preparation and certification of hijiki reference material, NMIJ CRM 7405-a, from the edible marine algae hijiki (Hizikia fusiforme).

    Science.gov (United States)

    Narukawa, Tomohiro; Inagaki, Kazumi; Zhu, Yanbei; Kuroiwa, Takayoshi; Narushima, Izumi; Chiba, Koichi; Hioki, Akiharu

    2012-02-01

    A certified reference material, NMIJ CRM 7405-a, for the determination of trace elements and As(V) in algae was developed from the edible marine hijiki (Hizikia fusiforme) and certified by the National Metrology Institute of Japan (NMIJ), the National Institute of Advanced Industrial Science and Technology (AIST). Hijiki was collected from the Pacific coast in the Kanto area of Japan, and was washed, dried, powdered, and homogenized. The hijiki powder was placed in 400 bottles (ca. 20 g each). The concentrations of 18 trace elements and As(V) were determined by two to four independent analytical techniques, including (ID)ICP-(HR)MS, ICP-OES, GFAAS, and HPLC-ICP-MS using calibration solutions prepared from the elemental standard solution of Japan calibration service system (JCSS) and the NMIJ CRM As(V) solution, and whose concentrations are certified and SI traceable. The uncertainties of all the measurements and preparation procedures were evaluated. The values of 18 trace elements and As(V) in the CRM were certified with uncertainty (k = 2).

  3. Preparation

    Directory of Open Access Journals (Sweden)

    M.M. Dardir

    2014-03-01

    Full Text Available Some hexanamide-mono and di-linoleniate esters were prepared by the reaction of linolenic acid and hexanamide (derived from the reaction of hexanoic acid and diethanolamine. The chemical structure for the newly prepared hexanamide-mono and di-linoleniate esters were elucidated using elemental analysis, (FTIR, H 1NMR and chemical ionization mass spectra (CI/Ms spectroscopic techniques. The results of the spectroscopic analysis indicated that they were prepared through the right method and they have high purity. The new prepared esters have high biodegradability and lower toxicity (environmentally friendly so they were evaluated as a synthetic-based mud (ester-based mud for oil-well drilling fluids. The evaluation included study of the rheological properties, filtration and thermal properties of the ester based-muds formulated with the newly prepared esters compared to the reference commercial synthetic-based mud.

  4. An evaluation framework for pre-distribution strategies of certificates in VANETs

    NARCIS (Netherlands)

    Feiri, Michael; Petit, Jonathan; Kargl, Frank

    2014-01-01

    Security and privacy in vehicular communication are expected to be ensured by the pervasive use of pseudonymous certificates and signed messages. The design and establishment of necessary public key infrastructure and hierarchies of cer- tificate authorities is ongoing in industry consortia, such as

  5. Preparation and evaluation of biocomposites as wound dressing material.

    Science.gov (United States)

    Ramnath, V; Sekar, S; Sankar, S; Sankaranarayanan, C; Sastry, T P

    2012-12-01

    Collagen was isolated from the chrome containing leather waste (CCLW) which is a major solid waste in leather industry. Composite films were made using sago starch (SG), soya protein (SY), and collagen (C) and were cross linked with glutaraldehyde (G).The films prepared were characterized for their physico chemical properties like tensile strength, infrared spectra, thermogravimetric analysis, surface morphology, and water absorption studies. Better mechanical properties and surface morphology were observed for SG-SY-G-C films compared to other films prepared using collagen. The composite films prepared were used as wound dressing material on the experimental wounds of rats and healing pattern was evaluated using planimetric, biochemical, and histopathological studies. These studies have revealed better wound healing capacity of SG-SY-G-C film and utilization of CCLW in the preparation of value added product like wound dressing material.

  6. 40 CFR 265.93 - Preparation, evaluation, and response.

    Science.gov (United States)

    2010-07-01

    ..., location, or depth of the monitoring wells to bring the ground-water monitoring system into compliance with..., STORAGE, AND DISPOSAL FACILITIES Ground-Water Monitoring § 265.93 Preparation, evaluation, and response... comprehensive ground-water monitoring program (than that described in §§ 265.91 and 265.92)......

  7. Small College Teacher Preparation Program Evaluations: A Multiple Case Study

    Science.gov (United States)

    Saynes, Kathryn

    2013-01-01

    The methods of evaluating teacher preparation programs are becoming increasingly more reliant on student test data. These test data driven formats, however, are not appropriate for small colleges. Small colleges are currently left off the Tennessee Report Card on the Effectiveness of Teacher Training Programs because the colleges did not produce…

  8. Evaluation and optimisation of preparative semi-automated electrophoresis systems for Illumina library preparation.

    Science.gov (United States)

    Quail, Michael A; Gu, Yong; Swerdlow, Harold; Mayho, Matthew

    2012-12-01

    Size selection can be a critical step in preparation of next-generation sequencing libraries. Traditional methods employing gel electrophoresis lack reproducibility, are labour intensive, do not scale well and employ hazardous interchelating dyes. In a high-throughput setting, solid-phase reversible immobilisation beads are commonly used for size-selection, but result in quite a broad fragment size range. We have evaluated and optimised the use of two semi-automated preparative DNA electrophoresis systems, the Caliper Labchip XT and the Sage Science Pippin Prep, for size selection of Illumina sequencing libraries.

  9. Preparation and Evaluation of Stomatitis Film Using Xyloglucan Containing Loperamide.

    Science.gov (United States)

    Kawano, Yayoi; Sasatsu, Masanaho; Mizutani, Ayako; Hirose, Kaoru; Hanawa, Takehisa; Onishi, Hiraku

    2016-06-01

    Stomatitis induced by radiation therapy or cancer chemotherapy is a factor in sleep disorders and/or eating disorders, markedly decreasing patient quality of life. In recent years, disintegrating oral films that are easy to handle have been developed; therefore, we focused on the formulation of these films. We prepared an adhesive film for the oral cavity using xyloglucan (Xylo), which is a water-soluble macromolecule. We used loperamide, which has been reported to relieve pain caused by stomatitis effectively, as a model drug in this study. Films were prepared from Xylo solutions (3% (w/w)) and hypromellose (HPMC) solutions (1% (w/w)). Xylo and HPMC solutions were mixed at ratios of 1 : 1, 2 : 1, or 3 : 1 for each film, and films 2×2 cm weighing 3 g were prepared and dried at 37°C for 24 h. Physicochemical properties such as strength, adhesiveness, disintegration behavior, and dissolution of loperamide from films were evaluated. Films prepared from Xylo solution alone had sufficient strength and mucosal adhesion. On the other hand, films prepared from a mixture of Xylo and HPMC were inferior to those made from Xylo, but showed sufficient strength and mucosal adhesion and were flexible and easy to handle. The films prepared in this study are useful as adhesion films in the oral cavity.

  10. Wineries Evaluation of Costs and Benefits of Sustainability Certification Program: The Case of Terra Vitis in France.

    Science.gov (United States)

    Jourjon, Frederique; Chou, Hsia-Chi; Gezart, Anna; Kadison, Amy E; Martinat, Lea; Pomarici, Eugenio; Vecchio, Riccardo

    2016-01-01

    The current paper analyses the evaluation of the costs and benefits of French wineries (N=69) participating in the sustainability program Terra Vitis, a widespread environmental certification scheme within the French wine industry. An online questionnaire was sent to all Terra Vitis participants, in order to analyse the evaluation of economic costs and benefits (together with environmental benefits) as perceived by wineries. Our findings reveal that older participants in the scheme (over 5 years), firms with higher export share (>40% of annual turnover) and cooperative wineries tend to be keener to assign a positive evaluation to the benefits/costs ratio in both the vineyard and the winery. In the context of increasing concerns regarding the economic and environmental performance of the French agricultural sector, such findings and also the patent research could be useful for policy makers and entrepreneurs in defining mainstream normative and corporate strategies. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  11. Final safety evaluation report related to the certification of the advanced boiling water reactor design. Volume 1: Main report

    Energy Technology Data Exchange (ETDEWEB)

    1994-07-01

    This safety evaluation report (SER) documents the technical review of the US Advanced Boiling Water Reactor (ABWR) standard design by the US Nuclear Regulatory Commission (NRC) staff. The application for the ABWR design was initially submitted by the General Electric Company, now GE Nuclear Energy (GE), in accordance with the procedures of Appendix O of Part 50 of Title 10 of the Code of Federal Regulations (10 CFR Part 50). Later GE requested that its application be considered as an application for design approval and subsequent design certification pursuant to 10 CFR {section} 52.45. The ABWR is a single-cycle, forced-circulation, boiling water reactor (BWR) with a rated power of 3,926 megawatts thermal (MWt) and a design power of 4,005 MWt. To the extent feasible and appropriate, the staff relied on earlier reviews for those ABWR design features that are substantially the same as those previously considered. Unique features of the ABWR design include internal recirculation pumps, fine-motion control rod drives, microprocessor-based digital logic and control systems, and digital safety systems. On the basis of its evaluation and independent analyses, the NRC staff concludes that, subject to satisfactory resolution of the confirmatory items identified in Section 1.8 of this SER, GE`s application for design certification meets the requirements of Subpart B of 10 CFR Part 52 that are applicable and technically relevant to the US ABWR standard design.

  12. Final safety evaluation report related to the certification of the advanced boiling water reactor design. Volume 2: Appendices

    Energy Technology Data Exchange (ETDEWEB)

    1994-07-01

    This safety evaluation report (SER) documents the technical review of the US Advanced Boiling Water Reactor (ABWR) standard design by the US Nuclear Regulatory Commission (NRC) staff. The application for the ABWR design was initially submitted by the General Electric Company, now GE Nuclear Energy (GE), in accordance with the procedures of Appendix O of Part 50 of Title 10 of the code of Federal Regulations (10 CFR Part 50). Later GE requested that its application be considered as an application for design approval and subsequent design certification pursuant to 10 CFR {section} 52.45. The ABWR is a single-cycle, forced-circulation, boiling water reactor (BWR) with a rated power of 3,926 megawatts thermal (MWt) and a design power of 4,005 MWt. To the extent feasible and appropriate, the staff relied on earlier reviews for those ABWR design features that are substantially the same as those previously considered. Unique features of the ABWR design include internal recirculation pumps, fine-motion control rod drives, microprocessor-based digital logic and control systems, and digital safety systems. On the basis of its evaluation and independent analyses, the NRC staff concludes that, subject to satisfactory resolution of the confirmatory items identified in Section 1.8 of this SER, GE`s application for design certification meets the requirements of Subpart B of 10 CFR Part 52 that are applicable and technically relevant to the US ABWR standard design.

  13. Final safety evaluation report related to the certification of the Advanced Boiling Water Reactor design. Supplement 1

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-05-01

    This report supplements the final safety evaluation report (FSER) for the US Advanced Boiling Water Reactor (ABWR) standard design. The FSER was issued by the US Nuclear Regulatory Commission (NRC) staff as NUREG-1503 in July 1994 to document the NRC staff`s review of the US ABWR design. The US ABWR design was submitted by GE Nuclear Energy (GE) in accordance with the procedures of Subpart B to Part 52 of Title 10 of the Code of Federal Regulations. This supplement documents the NRC staff`s review of the changes to the US ABWR design documentation since the issuance of the FSER. GE made these changes primarily as a result of first-of-a-kind-engineering (FOAKE) and as a result of the design certification rulemaking for the ABWR design. On the basis of its evaluations, the NRC staff concludes that the confirmatory issues in NUREG-1503 are resolved, that the changes to the ABWR design documentation are acceptable, and that GE`s application for design certification meets the requirements of Subpart B to 10 CFR Part 52 that are applicable and technically relevant to the US ABWR design.

  14. Preparation and evaluation of gastroretentive floating tablets of acyclovir.

    Science.gov (United States)

    Garg, Rajeev; Gupta, G D

    2009-10-01

    The present study performed by preparation and evaluation of floating tablets of Acyclovir as model drug for prolongation of gastric residence time. Floating effervescent tablets were formulated by various materials like hydroxypropyl methylcellulose K 4M, K 15M, psyllium husk, swelling agent as crospovidone and microcrystalline cellulose and gas generating agent like sodium bicarbonate and citric acid and evaluated for floating properties, swelling characteristics and in vitro drug release studies. Floating noneffervescent tablets were prepared by polypropylene foam powder and different matrix forming polymers like HPMC K 4M, Carbopol 934P, xanthan gum and sodium alginate. In vitro drug release studies were performed and drug release kinetics evaluated using the linear regression method was found to follow both the Higuchi and the Korsmeyer and Peppas equation. The drug release mechanism was found fickian type in most of the formulations.

  15. Preparation and evaluation of buccal bioadhesive films containing clotrimazole.

    Science.gov (United States)

    Singh, S; Jain, S; Muthu, M S; Tiwari, S; Tilak, R

    2008-01-01

    Buccal bioadhesive films, releasing topical drugs in the oral cavity at a slow and predetermined rate, provide distinct advantages over traditional dosage forms. The aim of present study was to prepare and evaluate buccal bioadhesive films of clotrimazole for oral candidiasis. The film was designed to release the drug at a concentration above the minimum inhibitory concentration for a prolonged period of time so as to reduce the frequency of administration of the available conventional dosage forms. The different proportions of sodium carboxymethylcellulose and carbopol 974P (CP 974P) were used for the preparation of films. Carbopol was used to incorporate the desired bioadhesiveness in the films. The films were prepared by solvent casting method and evaluated for bioadhesion, in vitro drug release and effectiveness against Candida albicans. In vitro drug release from the film was determined using a modified Franz diffusion cell while bioadhesiveness was evaluated with a modified two-arm balance using rabbit intestinal mucosa as a model tissue. Films containing 5% CP 974P of the total polymer were found to be the best with moderate swelling along with favorable bioadhesion force, residence time and in vitro drug release. The microbiological studies revealed that drug released from the film could inhibit the growth of C. albicans for 6 h. The drug release mechanism was found to follow non-Fickian diffusion.

  16. Preparation and gamma scintigraphic evaluation of colon specific pellets of ketoprofen prepared by powder layering technology

    Directory of Open Access Journals (Sweden)

    M Subhabrota

    2011-03-01

    Full Text Available "n  "n Background and the purpose of the study:Multiparticulates by powder layering process have advantages of the uniform distribution of the binder solution, easy-to-clean pan and the possibility of applying the successive functional film coating using the same equipment. This study relates to a multiparticulate formulation comprising pellets with a multilayer of pectin-ethyl cellulose on non pareil seeds by powder layering technology. The pellets were prepared to target ketoprofen in colon based on the microbial enzyme dependent drug release mechanism. "nMethods: Multiparticulate formulation by powder layering technology was prepared by conventional pan coating process to evaluate the effect of 59% methoxylated pectin and 45 cps ethyl cellulose on coating label. The formulations were tagged with 99mTc-DTPA, a tracer in gamma scintigraphy study to evaluate the transit behavior of drug loaded pellets and compared with uncoated pellets to evaluate its specific release. "nResults: The transit behavior and scintigraphy image clearly indicates that the formulation can delay the drug release prior to colon. In albino rabbit, the coated pellets released drug in the colon indicating that site specificity has been achieved with pectin/ethyl cellulose coating at 1:2 ratio with 20% coating label. Major conclusion: Formulation containing pectin and ethyl cellulose with suitable coating label may be suitable as a coating formulation for colon delivery of ketoprofen and can be successfully evaluated by gamma scintigraphy method.

  17. Evaluation of the Community Health Nursing Course of First Year Proficiency Certificate Level Nursing in Nepal

    Directory of Open Access Journals (Sweden)

    Mandira Shahi

    2014-03-01

    Full Text Available INTRODUCTION: Community health is very much important in nursing education. It is essential because it maximizes the health status of individuals, families, groups and the community through direct approach with them. The main purpose of the study was to identify the gap in Community Health Nursing I course in Proficiency Certificate Level first year nursing program in Nepal. METHODS: Mix methods of research having qualitative and quantitative method were used in the study. Data were collected from 12 subject teachers, 35 nursing graduates and 61 Proficiency Certificate Level first year nursing students. The study used structured, five-point rating scale and open ended questions according to Strengths, Weaknesses, Opportunities and Threats analysis for the self-administered questionnaire. FINDINGS: Common view points of the three sector's respondents (student, nursing graduate and teachers regarding the strengths of curriculum are: curriculum is based on Primary Health Care approach and covers preventive and promotive aspects of health. Regarding weaknesses, they said that there is inadequate time for practice, there is lack of innovative methods and materials, the course didn't cover new trends of environmental pollution and changes, global warming, greenhouse effect, climate change and deforestation etc. Similarly, they added that curriculum is not revised regularly and there is insufficient supervision in field. Likewise, regarding opportunities, they said that there is job opportunity in social organization as Community Health Nursing/Public Health Nurse. Moreover, they said that there is lack of employment scope as threats point. CONCLUSION: The paper concludes that new issues and trends of community health nursing should be added, and curriculum should be revised regularly.

  18. An overview of Preparation, Evaluation and Applications of Multiple Emulsions

    Directory of Open Access Journals (Sweden)

    Sandip Prajapati

    2013-04-01

    Full Text Available Multiple emulsions are also known as emulsions of emulsions, liquid membrane system or double emulsion. Multiple emulsions are polydispersed systems where both oil in water & water in oil emulsions exist simultaneously. This review focuses on preparation, characterization and potential applications of multiple emulsions. Multiple emulsions can be classified as water-in oil-in water (W/O/W or oil-in-water-in-oil (O/W/O emulsions. This review described five methods to prepare multiple emulsions viz. two-step emulsification method, modified two-step emulsification method, phase inversion method, membrane emulsification & micro channel emulsification method. The Multiple emulsion is characterized by average globule size & size distribution, area of interfaces, number of globules, rheological evaluation, zeta potential, percentage drug entrapment, In-vitro drug release. Multiple emulsions have been proposed to have numerous uses including their use as prolonged drug delivery system.

  19. Testicular touch preparation cytology in the evaluation of male infertility

    Directory of Open Access Journals (Sweden)

    Isil Z Yildiz-Aktas

    2011-01-01

    Full Text Available Background: Male infertility is traditionally evaluated by tissue core biopsies of the testes. Touch preparations (TP of these biopsies have been infrequently used. The aim of this study is to report our experience with using testicular biopsy TP for the evaluation of male infertility. Materials and Methods: A retrospective search was performed for cases of testes biopsies with concurrent TP. These cases were evaluated for clinical information, specimen adequacy, and cytological-histological correlation. Results: A total of 39 cases were identified from men with a mean age of 34 years (range 23 to 50 years. TP slides were satisfactory for evaluation in 31 (89% cases, and less than optimal in four due to low cellularity, obscuring blood or air drying artifact. Cytopathology showed concordance with the biopsy in almost all cases. In one discordant case where the biopsies showed no active spermatogenesis, a rare sperm were identified on the TP. Conclusions: TP of the testis is a helpful adjunct to biopsy because of its ability to clearly evaluate all stages of spermatogenesis. These data demonstrate that TP cytopathology of the testes in our experience has an excellent correlation with both normal testicular biopsies and those showing pathological spermatogenesis, and in rare cases may provide added benefit in evaluating the presence of spermatogenesis for male infertility. Albeit uncommon, cytopathologists may be required to identify and evaluate spermatogenic elements in cytology specimens being submitted from men with infertility.

  20. An Evaluative Study of an ICT Module for a School Leadership and Management Preparation Program

    Science.gov (United States)

    Musgrave, Sarietjie; De Wet, Corene

    2017-01-01

    This study reports on findings of an evaluative study on the effectiveness of an information and communication technology (ICT) module that forms part of the Advanced Certificate in Education: School Leadership and Management program. The study was carried out among distance education (DE) students from the University of the Free State enrolled…

  1. Preparation and evaluation of functional foods in adjuvant arthritis

    Energy Technology Data Exchange (ETDEWEB)

    Al-Okbi, S. Y.; Mohamed, D. A.

    2012-07-01

    Adjuvant arthritis is an animal model that closely resembles rheumatoid arthritis in humans. It is a successful working model used to study new anti-inflammatory agents. In previous studies (animal and clinical) we have shown that evening primrose oil, fish oil and the methanol extract of date fruits and fenugreek seeds have anti-inflammatory activity and that the methanol extract of dates has an antioxidant effect. Based on these studies, the aim of the present study was to prepare 7 functional foods containing such bioactive fractions separately or in combination and to evaluate them in adjuvant arthritis in rats, study the stability of bioactive ingredients and evaluate their sensory properties. The studied biochemical parameters were erythrocyte sedimentation rate, erythrocyte superoxide dismutase, glutathione peroxidase and plasma copper, zinc and interlukin 2. Nutritional parameters, including body weight gain, food intake and food efficiency ratio were monitored during the feeding of the functional foods. The bioactive ingredients assessed were total phenolic contents and fatty acids. The results showed improvement in the biochemical parameters, body weight gain and food efficiency ratio of arthritic rats fed on the functional foods with different degrees. All the prepared functional foods were sensory accepted. The active ingredients showed stability during storage. In conclusion, all the tested functional foods showed promising antiinflammatory activity and were determined to be acceptable through sensory evaluation which means that their potential beneficial use as dietary supplements in rheumatoid arthritis patients may be recommended. (Author) 42 refs.

  2. Preparation and evaluation of gastroretentive floating tablets of Silymarin.

    Science.gov (United States)

    Garg, Rajeev; Gupta, Ghanshyam Das

    2009-06-01

    The present study performed by preparation and evaluation of floating tablets of Silymarin as model drug for prolongation of gastric residence time. Floating effervescent tablets were formulated by various materials like hydroxypropyl methylcellulose (HPMC) K 4M, K 15M, psyllium husk, swelling agent as crospovidone and microcrystalline cellulose and gas generating agent like sodium bicarbonate and citric acid and evaluated for floating properties, swelling characteristics and in vitro drug release studies. Floating noneffervescent tablets were prepared by polypropylene foam powder and different matrix forming polymers like HPMC K 4M, Carbopol 934P, xanthan gum and sodium alginate. In vitro drug release studies were performed and drug release kinetics evaluated using the linear regression method was found to follow both the Higuchi and the Korsemeyer and Peppas equation. The drug release mechanism was found fickian type in most of the formulations. The developed floating tablets of Silymarin may be used in clinic for prolonged drug release for at least 24 h, thereby improving the bioavailability and patient compliance.

  3. Preparation and Evaluation of PLGA-Coated Capsaicin Magnetic Nanoparticles.

    Science.gov (United States)

    Baskaran, Mrudhula; Baskaran, Padmamalini; Arulsamy, Navamoney; Thyagarajan, Baskaran

    2017-06-01

    Drugs used in the treatment of diseases can cause several unwanted systemic side effects. A site-specific drug delivery system can eliminate such consequences by delivering drugs to certain target areas of the body where therapeutic effects are required. Here we present the preparation and evaluation of magnetic nanoparticles of capsaicin, the active ingredient in chili peppers, coated with poly-L-lactide co-glycolide (PLGA), a FDA-approved biodegradable bioavailable polymer. PCMN were prepared by solvent-evaporation/coprecipitation technique and their physicochemical and pharmacological characteristics evaluated in vitro. Further, effective pain/inflammation therapeutics of PCMN in a mouse model of inflammation was also studied. We also prepared and evaluated the subcellular localization of PLGA coated fluorescence magnetic nanoparticle (PFMN) in vitro in HEK293 cells. Transmission electron microscopic images of PCMN showed that the size of the nanoparticles were of the order of 10-20 nm. PCMN showed approximately 9.29% drug loading and 89.15% encapsulation efficiencies. In vitro dissolution studies showed an increased solubility of capsaicin due to the nano-size of the PCMN, while PLGA coating allowed sustained release of capsaicin in vitro. The PCMN also reduced paw edema after injection in mice, and confocal microscopy revealed the successful intracellular localization of PLGA-coated fluorescein magnetic nanoparticles in HEK293 cells. The PCMN provided a sustained release of capsaicin in vitro and inhibited carrageenan-induced inflammatory pain in mouse model in vivo. These data suggest that PLGA coating of capsaicin magnetic nanoparticles have the potential to be amenable for a sustained release of capsaicin to relieve pain.

  4. Evaluation of “First Certificate Gold Course Book”: Evidence from Students of a Private English Institute in Iran

    Directory of Open Access Journals (Sweden)

    Sara Haghighi

    2014-07-01

    Full Text Available The purpose of this study was to evaluate the first certificate Gold course book (Aclam & Burgess, 2006 taught at Mehr English Institute in Lahijan (Guilan Province, Iran. The objectives of this book focused on developing a communicative competence, providing plenty of practice, including listening and reading texts to bring the course up to date, including writing tasks, etc. The sample consisted of 35 EFL learners who were selected through random sampling. Also, 6 teachers evaluated the textbook. The researcher used a quantitative research survey design which includes a researcher-made evaluation checklist on the basis of eight checklist references. The course book was evaluated according to five key aspects: design and organization, topics, skills and strategies, practical considerations and illustrations, and activities. Quantitative data was obtained through a five-point Likert scale questionnaire consisting of 23 items. Descriptive statistics including percentage were calculated for each item of the questionnaire. Findings indicated that majority of participants and teachers had positive views toward the textbook. It was concluded that despite having a few shortcomings, the course book was fairly successful according to its intended objectives. In the light of these findings, some pedagogical implications are discussed.

  5. Analytical, biopharmaceutical and regulatory evaluation of topical testosterone preparations.

    Science.gov (United States)

    Baert, B; Annavarapu, S; Burvenich, C; De Spiegeleer, B

    2009-05-01

    Testosterone-containing pharmaceutical products for topical use were obtained from the pharmacist or through the internet. The legal status of the different products obtained is discussed: some products through the internet were clearly a medicinal product according to the current definitions, while they are not registered as such. Assay and impurity profiles of each of the marketed samples were obtained using HPLC-UV and ESI-iontrap MS. The analytical results were evaluated relative to the reporting, identification and qualification thresholds as defined by the the International Conference on Harmonisation (ICH) and the European Pharmacopoeia (Ph. Eur.). Preparations with impurities above the qualification threshold were observed. Moreover, in vitro release profiles over an artificial membrane were obtained using a standardised cell in a paddle dissolution bath as well as in a static Franz diffusion cell, using phosphate buffered saline (PBS; pH 7.0) containing 5% bovine serum albumin (BSA) as dissolution or receptor fluid. This biopharmaceutical quality attribute differs significantly between the preparations tested. In conclusion, the equivalency of topical testosterone preparations is not assured, nor on their legal status, nor on their impurity profiling nor on their biopharmaceutical behaviour. This calls for an urgent trans-national product-class harmonisation approach.

  6. HPLC evaluation of diclofenac in transdermal therapeutic preparations.

    Science.gov (United States)

    Klimes, J; Sochor, J; Dolezal, P; Körner, J

    2001-04-17

    High-performance liquid chromatography (HPLC) was selected for analytical evaluation of sodium diclofenac in original transdermal therapeutic preparations containing adjuvant substances (capsaicin, hyoscyamine). After isolation from laminated adhesive patches, diclofenac was analysed on columns with reversed phase, using the mobile phase ethanol and phosphate buffer (pH 6.5) with an addition of tetrabutylammonium iodide and detection at 284 nm. Not only the total amount of diclofenac in the patch was evaluated, but HPLC methodology was also employed to select a suitable acceptor medium for permeation experiments. In patches manufactured in the tested series, HPLC was also employed to examine the release of diclofenac and its in vitro permeation through the human skin.

  7. Consistency of the French white certificates evaluation system with the framework proposed for the European energy services

    Energy Technology Data Exchange (ETDEWEB)

    Broc, J.S.; Bourges, B. [GEPEA UMR CNRS, 6144, Ecole des Mines de Nantes, Nantes (France); Osso, D.; Baudry, P. [Electricite de France - R and D, Ecuelles (France); Adnot, J. [Centre for Energy and Processes, Mines ParisTech, Paris (France); Bodineau, L. [Climate Department, ADEME, Angers (France)

    2011-06-15

    According to the directive on energy end-use efficiency and energy services (ESD), the European Member States shall adopt a national indicative energy savings target of 9% (or beyond) in 2016. The issue of the energy savings evaluation is crucial for its implementation. The French White Certificates (FWC) scheme is one of the important measures for France to fulfill its ESD target. However, the accountings of energy savings in the FWC scheme and in the ESD are different. Therefore, an analysis of the consistency of the two systems is needed. A concrete example of actions on residential buildings is used to illustrate the challenges for policy marker and stakeholders to set harmonized evaluation rules. The FWC and ESD calculations appear to be consistent from a physics point of view, as long as calculations are well-documented. But due to differences in the policy objectives, calculation routines may be necessary to convert national energy savings unit (e.g., kWh cumac) into supranational energy savings unit (e.g., ESD kWh). Finally, the work done to establish a transparent evaluation system brings additional benefits (e.g., increased visibility and quality of the actions), which will improve the results of the energy efficiency policies on long term.

  8. Evaluation of a Certification Process for Community Nurses Involved in Sex and Relationship Education

    Science.gov (United States)

    Chalmers, Helen; Aggleton, Peter; Tyrer, Paul

    2006-01-01

    Purpose: Alongside teachers, community nurses have been identified as having an important role to play in the provision of school-based personal, social and health education (PSHE) and sex and relationships education (SRE). However, there currently exist few programmes of preparation for this work that recognise the specific contribution of…

  9. Flavored whey drinks: preparation and evaluation of selected parameters

    Directory of Open Access Journals (Sweden)

    Alica Bobková

    2016-10-01

    Full Text Available The aim of our work was the preparation of fermented flavored whey beverages using whey, probiotic cultures and suitable flavors. Whey fermentation by lactic acid bacteria appears to be an interesting alternative to improve the properties of whey and thus to offer its exceptional nutritional value, not only because of whey protein contained, but also due to the valuable products produced by microorganisms. Pasteurized whey was enriched by the addition of sheep culture ZS-25, which was isolated from sheep cheese and contains more varieties of species Lactococcus lactis, and also by the probiotic culture containing the microorganisms of Bifidobacterium sp., Lactobacillus acidophilus, Streptococcus thermophilus. We focused our attention to select the appropriate type and concentration of flavor as well. Sensory evaluation was performed by the committee consisting of five intentionally selected professionally qualified persons. Taste of fermented whey drink was main observed property. Assessment of prepared whey drinks was repeated four times, each group consisted of 5 samples according to the following structure: sample A - whey drink with culture without flavoring, sample B - whey drink with culture and exotic flavor, sample C - whey drink with the culture and flavor of cranberry-grape-strawberry, sample D - whey drink with the culture and peach-orange flavor, sample E - whey drink with the culture and strawberry flavor. Flavored fermented whey beverages were sensorially evaluated. The order of individual samples determined by the evaluators was statistically processed by the Friedman test. Table value for this test for five evaluators and 5 samples is 8.99. The calculated values for all four sensory evaluations we performed, were higher than 8.99, so we rejected the null hypothesis and proved that among the different flavored whey drinks it is statistically significant difference. Overall, the worst evaluated was whey drink without flavoring. As

  10. Survey of Fire Detection Technologies and System Evaluation/Certification Methodologies and Their Suitability for Aircraft Cargo Compartments

    Science.gov (United States)

    Cleary, T.; Grosshandler, W.

    1999-01-01

    As part of the National Aeronautics and Space Administration (NASA) initiated program on global civil aviation, NIST is assisting Federal Aviation Administration in its research to improve fire detection in aircraft cargo compartments. Aircraft cargo compartment detection certification methods have been reviewed. The Fire Emulator-Detector Evaluator (FE/DE) has been designed to evaluate fire detection technologies such as new sensors, multi-element detectors, and detectors that employ complex algorithms. The FE/DE is a flow tunnel that can reproduce velocity, temperature, smoke, and Combustion gas levels to which a detector might be exposed during a fire. A scientific literature survey and patent search have been conducted relating to existing and emerging fire detection technologies, and the potential use of new fire detection strategies in cargo compartment areas has been assessed. In the near term, improved detector signal processing and multi-sensor detectors based on combinations of smoke measurements, combustion gases and temperature are envisioned as significantly impacting detector system performance.

  11. Preparation and Evaluation of Inhalable Itraconazole Chitosan Based Polymeric Micelles

    Directory of Open Access Journals (Sweden)

    Esmaeil Moazeni

    2012-12-01

    Full Text Available Background: This study evaluated the potential of chitosan based polymeric micelles as a nanocarrier system for pulmonary delivery of itraconazole (ITRA.Methods: Hydrophobically modified chitosan were synthesized by conjugation of stearic acid to the hydrophilic depolymerized chitosan. FTIR and 1HNMR were used to prove the chemical structure and physical properties of the depolymerized and the stearic acid grafted chitosan. ITRA was entrapped into the micelles and physicochemical properties of the micelles were investigated. Fluorescence spectroscopy, dynamic laser light scattering andtransmission electron microscopy were used to characterize the physicochemical properties of the prepared micelles. The in vitro pulmonary profile of polymeric micelles was studied by an air-jet nebulizer connected to a twin stage impinger.Results: The polymeric micelles prepared in this study could entrap up to 43.2±2.27 μg of ITRA per milliliter. All micelles showed mean diameter between 120–200 nm. The critical micelle concentration of the stearic acid grafted chitosan was found to be 1.58×10-2 mg/ml. The nebulization efficiency was up to 89% and the fine particle fraction (FPF varied from 38% to 47%. The micelles had enough stability to remain encapsulation of the drug during nebulization process.Conclusions: In vitro data showed that stearic acid grafted chitosan based polymeric micelles has a potential to be used as nanocarriers for delivery of itraconazole through inhalation.

  12. Evaluation of two different dendritic cell preparations with BCG reactivity

    Directory of Open Access Journals (Sweden)

    Fol Marek

    2016-01-01

    Full Text Available Dendritic cells (DCs play a key-role in the immune response against intracellular bacterial pathogens, including mycobacteria. Monocyte-derived dendritic cells (MoDCs are considered to behave as inflammatory cell populations. Different immunomagnetic methods (positive and negative can be used to purify monocytes before their in vitro differentiation and their culture behavior can be expected to be different. In this study we evaluated the reactivity of two dendritic cell populations towards the Bacillus Calmette-Guérin (BCG antigen. Monocytes were obtained from the blood of healthy donors, using positive and negative immunomagnetic separation methods. The expression of DC-SIGN, CD86, CD80, HLA-DR and CD40 on MoDCs was estimated by flow cytometry. The level of IL-12p70, IL-10 and TNF-α was measured by ELISA. Neither of the tested methods affected the surface marker expression of DCs. No significant alteration in immunological response, measured by cytokine production, was noted either. After BCG stimulation, the absence of IL-12, but the IL-23 production was observed in both cell preparations. Positive and negative magnetic separation methods are effective techniques to optimize the preparation of monocytes as the source of MoDCs for potential clinical application.

  13. Preparation and evaluation of controlled release tablets of carvedilol

    Directory of Open Access Journals (Sweden)

    Varahala Setti M

    2009-01-01

    Full Text Available The objective of the present investigation is to design and evaluate controlled release tablets of carvedilol, employing synthetic polymers like polyethylene oxides, of different molecular weights as release retarding materials and to select the optimized formulation based on the pharmacokinetics of carvedilol. Matrix tablets each containing 80 mg of carvedilol were formulated employing PEO N60 K, PEO 301, and PEO 303 as release-retarding polymers and β Cyclodextrin and HP β cyclodextrin as release modulators from the matrix. Carvedilol release from the formulated tablets was very slow. Hence the release was modulated with the use of cyclodextrins. The dissolution from the matrix tablets was spread over more than 24 hours and depended on the type of polymer, its concentration and the type of cyclodextrin used. All the matrix tablets prepared using polyethylene oxides showed very good controlled release over more than 24 hours. The matrix tablets prepared using HP β cyclodextrin showed a higher dissolution rate and gave a dissolution profile that was comparable to the theoretical sustained release needed for once-a-day administration of carvedilol. The drug release mechanism from the matrix tablets was found to be quasi Fickian mechanism.

  14. Preparation and evaluation of soft gellan gum gel containing paracetamol

    Directory of Open Access Journals (Sweden)

    Gohel M

    2009-01-01

    Full Text Available The objective of this study was to develop soft paracetamol gel using gellan gum as a gelling agent and sodium citrate as a source of cation. Different batches were prepared using three different concentrations of gellan gum (0.1, 0.3, and 0.5%, each with two different sodium citrate concentrations (0.3 and 0.5%. The consistency of the paracetamol gel was dependent on the concentration of gellan gum, sodium citrate and co-solute. The results of dissolution study of soft gel containing 0.3% gellan gum and 0.3% sodium citrate revealed that paracetamol was completely released in 30 min. Polyethylene glycol 400 worked as a solubilizer for paracetamol. All the gels possessed acceptable sensory characteristics when evaluated by human volunteers. Short term stability study carried out for four weeks at different temperatures revealed no considerable changes in performance characteristics of developed optimized formulation.

  15. Preparation and evaluation of veterinary 20% injectable solution of tylosin

    OpenAIRE

    2010-01-01

    A veterinary injectable aqueous solution of the antibiotic tylosin at a concentration of 20% was prepared under aseptic conditions in dark glass bottles each containing 100 ml. The preparation was intended for animal use only. It contained 200 g tylosin tartrate, 500 ml propylene glycol, benzyl alcohol 40 ml as a preservative and water for injection up to 1000 ml. The preparation was clear yellow viscous aqueous solution free from undesired particles. The preparation complied with the require...

  16. PREPARATION AND IN-VITRO EVALUATION OF BACLOFEN LOADED MICROSPHERES

    Directory of Open Access Journals (Sweden)

    Polepalle Madhulatha

    2012-09-01

    Full Text Available The present study was aimed to prepare Baclofen microspheres for sustained release using various polymers such as ethyl cellulose (hydrophobic, hydroxy propyl methyl cellulose (hydrophilic by employing solvent evaporation technique. Drug and excipients compatibility was studied by Fourier Transform Infrared Spectroscopy and no incompatibility was observed. The obtained microspheres were evaluated for the percent drug content, entrapment efficiency and in-vitro dissolution studies. The entrapment efficiency of the obtained formulations was in between 66-88% and in-vitro release of F7 formulation showed 93% in 24 hrs. Scanning Electron Microscopy, Differential Scanning Calorimetry and X-Ray Diffraction studies were performed for F7 formulation. From the results Scanning Electron Microscopy of reveals that microspheres was found in spherical and porous nature. X-Ray Diffraction studies results showed baclofen was in amorphous form which was further confirmed by Differential Scanning Calorimetry. The curve fitting data revealed that the release of obtained formulations follows mixed order kinetics with non‐fickian type of drug release (anomalous.

  17. Thick film traps with an irregular film. Preparation and evaluation.

    Science.gov (United States)

    Kloskowski, Adam; Pettersson, Johan; Roeraade, Johan

    2004-05-07

    A new method for preparation of sorbent-based ultra-thick film traps for concentration of trace volatile components from gaseous matrices is described. The procedure is based on blowing a prepolymer (polydimethylsiloxane) through a capillary tube, forming an irregular film of stationary phase. Subsequently, the prepolymer is immobilized in a few seconds by heating to 200 degrees C. Evaluation of the performance of the new traps showed that the loss of efficiency, compared to regular smooth film traps is only on the order of 20-30%. In terms of breakthrough volume, this loss in performance is rather insignificant. The technology is extremely simple and allows a rapid and cheap production of a large number of ultra-thick film traps, even in non-specialized laboratories. The method can be applied to any type of cross-linkable stationary phase, thereby expanding the scope of sorbent-based trapping and preconcentration concept. Many applications are anticipated in trace and ultra-trace analysis in a wide range of fields, such as environmental chemistry, polymers, food and process analysis.

  18. Preparation and evaluation of functional foods in adjuvant arthritis

    Directory of Open Access Journals (Sweden)

    Al-Okbi, S. Y.

    2012-10-01

    Full Text Available Adjuvant arthritis is an animal model that closely resembles rheumatoid arthritis in humans. It is a successful working model used to study new anti-inflammatory agents. In previous studies (animal and clinical we have shown that evening primrose oil, fish oil and the methanol extract of date fruits and fenugreek seeds have anti-inflammatory activity and that the methanol extract of dates has an antioxidant effect. Based on these studies, the aim of the present study was to prepare 7 functional foods containing such bioactive fractions separately or in combination and to evaluate them in adjuvant arthritis in rats, study the stability of bioactive ingredients and evaluate their sensory properties. The studied biochemical parameters were erythrocyte sedimentation rate, erythrocyte superoxide dismutase, glutathione peroxidase and plasma copper, zinc and interlukin 2. Nutritional parameters, including body weight gain, food intake and food efficiency ratio were monitored during the feeding of the functional foods. The bioactive ingredients assessed were total phenolic contents and fatty acids. The results showed improvement in the biochemical parameters, body weight gain and food efficiency ratio of arthritic rats fed on the functional foods with different degrees. All the prepared functional foods were sensory accepted. The active ingredients showed stability during storage. In conclusion, all the tested functional foods showed promising antiinflammatory activity and were determined to be acceptable through sensory evaluation which means that their potential beneficial use as dietary supplements in rheumatoid arthritis patients may be recommended.

    La artritis adyuvante es un modelo utilizado en animales y se caracteriza por ser muy parecida a la artritis reumatoide en humanos. Se trata de un modelo de trabajo utilizado con éxito para estudiar nuevos agentes anti-inflamatorios. En estudios previos (animales y clínica hemos demostrado que

  19. 40 CFR 61.208 - Certification requirements.

    Science.gov (United States)

    2010-07-01

    ... Emissions From Phosphogypsum Stacks § 61.208 Certification requirements. (a)(1) The owner or operator of a stack from which phosphogypsum will be removed and distributed in commerce pursuant to § 61.204, § 61.205, or § 61.206 shall prepare a certification document for each quantity of phosphogypsum which...

  20. Evaluation of research methods to study domestic food preparation

    NARCIS (Netherlands)

    Bongoni, R.; Verkerk, R.; Dekker, M.; Steenbekkers, B.

    2015-01-01

    Purpose – Domestic preparation practices influence the sensory properties and nutritional composition of food products. Information on the variability in actual domestic preparation practices is needed to assess the influence of applied conditions on the sensory and nutritional quality of food. The

  1. Evaluation of research methods to study domestic food preparation

    NARCIS (Netherlands)

    Bongoni, R.; Verkerk, R.; Dekker, M.; Steenbekkers, B.

    2015-01-01

    Purpose – Domestic preparation practices influence the sensory properties and nutritional composition of food products. Information on the variability in actual domestic preparation practices is needed to assess the influence of applied conditions on the sensory and nutritional quality of food. The

  2. Preparation and evaluation of veterinary 20% injectable solution of tylosin

    Directory of Open Access Journals (Sweden)

    Fouad K. Mohammad

    2010-02-01

    Full Text Available A veterinary injectable aqueous solution of the antibiotic tylosin at a concentration of 20% was prepared under aseptic conditions in dark glass bottles each containing 100 ml. The preparation was intended for animal use only. It contained 200 g tylosin tartrate, 500 ml propylene glycol, benzyl alcohol 40 ml as a preservative and water for injection up to 1000 ml. The preparation was clear yellow viscous aqueous solution free from undesired particles. The preparation complied with the requirements for injectable solutions. It was active in vitro against E. coli (JM83. The preparation of 20% tylosin solution was safe under field conditions in treating sheep and cattle suffering from pneumonia at the dose rate of 1 ml/20 kg body weight, intramuscularly/ day for 3 successive days. In conclusion, we presented the know-how of a veterinary formulation of injectable solution of 20% tylosin for clinical use in ruminants. [Vet. World 2010; 3(1.000: 5-7

  3. Evaluation preparation technology of Xiaochaihu granules using fingerprint-peak pattern matching%Evaluation preparation technology of Xiaochaihu granules using fingerprint-peak pattern matching

    Institute of Scientific and Technical Information of China (English)

    Yu-Qiong Wu; Yu- Qiang Gou; Jing Han; Ying-Yan Bi; Shi-Lan Feng; Fang-Di Hu; Chun- Ming Wang

    2011-01-01

    An approach was proposed to evaluate preparation technology by means of fingerprint-peak matching technology of high performance liquid chromatography with diode array detector (HPLC-DAD). Similarity and hierarchical clustering analysis (HCA) were applied

  4. Initial Teacher Certification Testing in Massachusetts: A Case of the Tail Wagging the Dog.

    Science.gov (United States)

    Flippo, Rona F.; Riccards, Michael P.

    2000-01-01

    An evaluation of the Massachusetts Educator Certification Test has revealed unforeseen, counterproductive consequences. Teacher preparation colleges are adjusting curricular emphases to teach to a test of dubious validity and are inadvertently excluding substantial portions of enrollees to boost test scores. Minorities are failing at higher rates.…

  5. 19 CFR 10.234 - Certificate of Origin.

    Science.gov (United States)

    2010-04-01

    ... Origin must be prepared by the exporter in the CBTPA beneficiary country. Where the CBTPA beneficiary... 19 Customs Duties 1 2010-04-01 2010-04-01 false Certificate of Origin. 10.234 Section 10.234... Certificate of Origin. A Certificate of Origin as specified in § 10.236 must be employed to certify that...

  6. Recent advances in liposomal dry powder formulations: preparation and evaluation.

    Science.gov (United States)

    Misra, Ambikanandan; Jinturkar, Kaustubh; Patel, Deepa; Lalani, Jigar; Chougule, Mahavir

    2009-01-01

    Liposomal drug dry powder formulations have shown many promising features for pulmonary drug administration, such as selective localization of drug within the lung, controlled drug release, reduced local and systemic toxicities, propellant-free nature, patient compliance, high dose carrying capacity, stability and patent protection. Critical review of the recent developments will provide a balanced view on benefits of liposomal encapsulation while developing dry powder formulations and will help researchers to update themselves and focus their research in more relevant areas. In liposomal dry powder formulations (LDPF), drug encapsulated liposomes are homogenized, dispersed into the carrier and converted into dry powder form by using freeze drying, spray drying and spray freeze drying. Alternatively, LDPF can also be formulated by supercritical fluid technologies. On inhalation with a suitable inhalation device, drug encapsulated liposomes get rehydrated in the lung and release the drug over a period of time. The prepared LDPF are evaluated in vitro and in vivo for lung deposition behavior and drug disposition in the lung using a suitable inhaler device. The most commonly used liposomes are composed of lung surfactants and synthetic lipids. Delivery of anticancer agents for lung cancer, corticosteroids for asthma, immunosuppressants for avoiding lung transplantation rejection, antifungal drugs for lung fungal infections, antibiotics for local pulmonary infections and cystic fibrosis and opioid analgesics for pain management using liposome technology are a few examples. Many liposomal formulations have reached the stage of clinical trials for the treatment of pulmonary distress, cystic fibrosis, lung fungal infection and lung cancer. These formulations have given very promising results in both in vitro and in vivo studies. However, modifications to new therapies for respiratory diseases and systemic delivery will provide new challenges in conducting well

  7. Preparation and in vitro evaluation of hydrophilic fenretinide nanoparticles.

    Science.gov (United States)

    Ledet, Grace A; Graves, Richard A; Glotser, Elena Y; Mandal, Tarun K; Bostanian, Levon A

    2015-02-20

    Fenretinide is an effective anti-cancer drug with high in vitro cytotoxicity and low in vivo systemic toxicity. In clinical trials, fenretinide has shown poor therapeutic efficacy following oral administration - attributed to its low bioavailability and solubility. The long term goal of this project is to develop a formulation for the oral delivery of fenretinide. The purpose of this part of the study was to prepare and characterize hydrophilic nanoparticle formulations of fenretinide. Three different ratios of polyvinyl pyrrolidone (PVP) to fenretinide were used, namely, 3:1, 4:1, and 5:1. Both drug and polymer were dissolved in a mixture of methanol and dichloromethane (2:23 v/v). Rotary evaporation was used to remove the solvents, and, following reconstitution with water, a high pressure homogenizer was used to form nanoparticles. The particle size and polydispersity index were measured before and after lyophilization. The formulations were studied by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and X-ray powder diffraction (XRPD). The effectiveness of the formulations was assessed by release studies and Caco-2 cell permeability assays. As the PVP content increased, the recovered particle size following lyophilization became more consistent with the pre-lyophilization particle size, especially for those formulations with less lactose. The DSC scans of the formulations did not show any fenretinide melting endotherms, indicating that the drug was either present in an amorphous form in the formulation or that a solid solution of the drug in PVP had formed. For the release studies, the highest drug release among the formulations was 249.2±35.5ng/mL for the formulation with 4:1 polymer-to-drug. When the permeability of the formulations was evaluated in a Caco-2 cell model, the mean normalized flux for each treatment group was significantly higher (p<0.05) from the fenretinide control. The formulation containing 4:1 polymer

  8. Alternative Teacher Certification and the New Professionalism: The Pre-Service Preparation of Mathematics Teachers in the New York City Teaching Fellows Program

    Science.gov (United States)

    Brantlinger, Andrew; Smith, Beverly

    2013-01-01

    Background/Context: For more than a decade, large alternative teacher certification programs (ATCP) such as the New York City Teaching Fellows (NYCTF) have provided qualified applicants with fast-track or "early entry" routes to paid teaching. While early-entry ATCPs enjoy powerful support in the public and private sectors, critics…

  9. "Pharmacodynamically evaluated bioequivalence of two preparations of Enalapril Maleate "

    Directory of Open Access Journals (Sweden)

    "Tajerzadeh H

    2001-07-01

    Full Text Available The bioequivalence of two preparations of enalapril maleate (20 mg tablets manufactured in Iran has been exploited in reference to a standard preparation (Xanef 20 tablets, MSD, Germany in 14 healthy volunteers. Following oral dosing of a single tablet of each of test and standard products, as a randomized crossover design with 10-day washout intervals, the blood samples were collected in predetermined time points and using a synthetic substrate, Hippuryl-Histidy-Leucine (HHL, the release of hippuric acid from the substrate was determined as Angiotensin-Converting-Enzyme (ACE activity of serum fractions. The percent of ACE inhibition in each sample was calculated and plotted against time, from which three pharmacodynamic parameters, i.e. Emax, tmax and AUC0-24 were derived. The results of statistical comparison of these parameters showed that both of the test preparations are bioequivalent with reference standard preparation.

  10. Sex and Relationships Education in Schools--Evaluation of a Pilot Programme for the Certification of Community Nurses

    Science.gov (United States)

    Chalmers, Helen; Tyrer, Paul; Aggleton, Peter

    2006-01-01

    Objective: In support of the UK Government's teenage pregnancy and sexual health strategies, a certificated programme of professional development for school nurses and other community nurses was developed to provide support for personal, social and health education (PSHE) work, including sex and relationships education (SRE), for young people.…

  11. Evaluation of new injection and cavity preparation model in local anesthesia teaching

    NARCIS (Netherlands)

    Yekta, S.S.; Lampert, F.; Kazemi, S.; Kazemi, R.; Brand, H.S.; Baart, J.A.; Mazandarani, M.

    2013-01-01

    The aim of this study was to evaluate a recently developed preclinical injection and cavity preparation model in local anesthesia. Thirty-three dental students administered an inferior alveolar nerve block injection in the model, followed by preparation on a tooth. The injection was evaluated by thr

  12. Evaluation of new injection and cavity preparation model in local anesthesia teaching

    NARCIS (Netherlands)

    Yekta, S.S.; Lampert, F.; Kazemi, S.; Kazemi, R.; Brand, H.S.; Baart, J.A.; Mazandarani, M.

    2013-01-01

    The aim of this study was to evaluate a recently developed preclinical injection and cavity preparation model in local anesthesia. Thirty-three dental students administered an inferior alveolar nerve block injection in the model, followed by preparation on a tooth. The injection was evaluated by

  13. [Evaluation of the potential mutagenic action of canned fish in oil prepared with Vakhtol' curing preparation].

    Science.gov (United States)

    Karpliuk, I A; Volkova, N A; Gogol', A T

    1980-01-01

    Bone marrow cells from CBA mice who had been given sprats cooked by means of the smoking preparation "Vakhtol" or by smoking over a month were subjected to cytogenetic analysis during the metaphase. No mutagenic changes were found in bone marrow cells.

  14. Safety evaluation of an α-cyclodextrin glycosyltranferase preparation

    NARCIS (Netherlands)

    Bär, A.; Krul, C.A.M.; Jonker, D.; Vogel, N. de

    2004-01-01

    Alpha-cyclodextrin glucosyltransferase (α-CGTase, EC 2.4.1.19) is an amylolytic enzyme used for the production of α-cyclodextrin (α-CD), a novel, soluble dietary fiber, from food-grade starch. The safety of an α-CGTase preparation obtained by batch fermentation from a recombinant strain of Escherich

  15. A Cavity Preparation Evaluation System in the Skill Assessment of Dental Students.

    Science.gov (United States)

    Zou, Huiru; Jin, Shufeng; Sun, Jianping; Dai, Yanmei

    2016-08-01

    The aim of this study was to compare a computerized, laser-scanning Cavity Preparation Skill Evaluation System (CPSES) with conventional teachers' eye-hand grading assessment of dental students' Class I cavity preparation evaluations. Thirty-eight cavity preparations of lower left first molars made by junior dental students at a dental school in China were tested from September 2013 to November 2014. The outline and retention form, smoothness, depth, wall angulation, and cavity margin index of the preparations were evaluated by CPSES and then by teachers' eye-hand grading. The mean difference in scores for each method was considered, as was the variability of scores within each method. Compared with the teachers' eye-hand grading method, CPSES provided objective evaluation results that had statistically significant differences (pcavity preparation skills and encourage students in their self-paced learning and independent practice.

  16. Preparation and evaluations in vitro of oxaliplatin polylactic acid nanoparticles.

    Science.gov (United States)

    Cui, Zhaoyuan; Sun, Yong; Liu, Xiaohong; Ju, Fang; Chen, Qian; Gao, Wen; Wei, Haitian

    2013-08-01

    The oxaliplatin nanoparticles were prepared with polylactic acid matrix, orthogonal test was applied to optimize the prescriptions, and the qualities of oxaliplatin nanoparticles were characterized by the shape, particle size, encapsulation efficiency (EE), and drug loading (DL). Oxaliplatin nanoparticle was prepared by solution replacement method. The formation of 0.25% Tween80, DMF-water 1:8 (v/v), oxaliplatin-polylactic acid 1:5 (w/w), and 20 mg/ml polylactic acid showed the suitable EE (17.4 ± 0.47%), DL (3.52 ± 0.07%). We observed the shape of oxaliplatin nanoparticles through SEM. The average size of the particles was 120.5 ± 8.7 nm, which was detected by N5 submicron particle size analyzer.

  17. Preparation and evaluation of functional foods in adjuvant arthritis

    OpenAIRE

    2012-01-01

    Adjuvant arthritis is an animal model that closely resembles rheumatoid arthritis in humans. It is a successful working model used to study new anti-inflammatory agents. In previous studies (animal and clinical) we have shown that evening primrose oil, fish oil and the methanol extract of date fruits and fenugreek seeds have anti-inflammatory activity and that the methanol extract of dates has an antioxidant effect. Based on these studies, the aim of the present study was to prepare 7 functio...

  18. Preparation of Zirconia Based Packing Material and Its Evaluation

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A new reversed-phase packing (C18-PBD-ZrO2) was prepared by depositing and cross-linking 1-octadecene (ODE or C18) and polybutadiene (PBD) onto the surface of porous zirconia microspheres (5~10 mm in diameter) which were synthesized by a sol-gel process. These novel column packings possess high mechanical and chemical stability,wider usable pH range and can be used to separate basic compounds with no observable peak tailing.

  19. Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes

    OpenAIRE

    Guiling Li; Chao Fan; Xinru Li; Yating Fan; Xiaoning Wang; Mei Li; Yan Liu

    2012-01-01

    The main objective of the present work was to prepare and assess dermal delivery of tacrolimus-loaded ethosomes versus classic liposomes. Both delivery systems were characterized for particle size, polydispersity index, and entrapment efficiency (EE), by dynamic laser diffraction and ultrafiltration or dialysis methods, respectively. The results indicated that presence of ethanol in the formulations affected the particle size. In addition, ultrafiltration method was selected to determine EE d...

  20. Preparation and evaluation of combination tablet containing incompatible active ingredients.

    Science.gov (United States)

    Wang, Xiaoyan; Cui, Fude; Yonezawa, Yorinobu; Sunada, Hisakazu

    2003-07-01

    Combination preparation plays an important role in clinical treatment because of its better and wider curative synergism and weaker side effects. However, the existence of incompatibility between active ingredients or between active ingredients and excipients presents a serious obstacle in the preparation of such combination solid dosage forms. In this study, aspirin and ranitidine hydrochloride, between which there existed a chemical interaction, were selected as model drugs. Aspirin powders without any additives were granulated with hydroxypropyl methyl cellulose (HPMC) water solution as a binder using a Wurster coating apparatus and the operation conditions were optimized by Artificial Neural Network (ANN) analysis. Under these conditions, the aspirin granules prepared showed good flowability and compressibility. On the other hand, ranitidine hydrochloride was coated with Aquacoat (ethyl cellulose aqueous dispersion) after preliminary granulation with the Wurster coating apparatus. The aspirin granules and coated ranitidine hydrochloride particles were compressed into tablets with suitable excipients. The combination tablets showed good dissolution, content uniformity and improved stability of active ingredients.

  1. Review of evaluations of crushing results for the seedbed preparation

    Directory of Open Access Journals (Sweden)

    Siegfried Anisch

    2016-06-01

    Full Text Available For evaluating the work results of tillage operations today only inaccurate parameters are available which cannot be readily measured. Thus, evaluating and comparing the work results of machines and developing suitable sensors are difficult. At the Technical University of Dresden, till 1990, research projects for determining aggregate size composition of soil have been done. Based on this work, this study will show suggestions for measuring, displaying and evaluation of soil crushing results depending on tillage work conditions.

  2. Final safety evaluation report related to the certification of the System 80{sup +} design (Docket No. 52-002). Volume 2, Chapters 15--22 and appendices

    Energy Technology Data Exchange (ETDEWEB)

    1994-08-01

    This final safety evaluation report (FSER) documents the technical review of the System 80+ standard design by the US Nuclear Regulatory Commission (NRC) staff. The application for the system 80+ design was submitted by Combustion Engineering, Inc., now Asea Brown Boveri-Combustion Engineering (ABB-CE) as an application for design approval and subsequent design certification pursuant to 10 CFR {section} 52.45. System 80+ is a pressurized water reactor with a rated power of 3914 megawatts thermal (MWt) and a design power of 3992 MWt at which accidents are analyzed. Many features of the System 80+ are similar to those of ABB-CE`s System 80 design from which it evolved. Unique features of the System 80+ design include: a large spherical, steel containment; an in-containment refueling water storage tank; a reactor cavity flooding system, hydrogen ignitors and a safety depressurization system for severe accident mitigation; a combustion gas turbine for an alternate ac source; and an advanced digitally based control room. On the basis of its evaluation and independent analyses, the NRC staff concludes that ABB-CE`s application for design certification meets the requirements of Subpart B of 10 CFR Part 52 that are applicable and technically relevant to the System 80+ standard design. This document, Volume 2, contains Chapters 15 through 22 and Appendices A through E.

  3. Final safety evaluation report related to the certification of the System 80{sup +} design (Docket No. 52-002). Volume 1, Chapters 1--14

    Energy Technology Data Exchange (ETDEWEB)

    1994-08-01

    This final safety evaluation report (FSER) documents the technical review of the System 80+ standard design by the US Nuclear Regulatory Commission (NRC) staff. The application for the System 80+ design was submitted by Combustion Engineering, Inc., now Asea Brown Boveri-Combustion Engineering (ABB-CE) as an application for design approval and subsequent design certification pursuant to 10 CFR {section} 52.45. System 80+ is a pressurized water reactor with a rated power of 3914 megawatts thermal (MWt) and a design power of 3992 MWt at which accidents are analyzed. Many features of the System 80+ are similar to those of Abb-CE`s System 80 design from which it evolved. Unique features of the System 80+ design included: a large spherical, steel containment; an in-containment refueling water storage tank; a reactor cavity flooding system, hydrogen ignitors, and a safety depressurization system for severe accident mitigation; a combustion gas turbine for an alternate ac source; and an advanced digitally based control room. On the basis of its evaluation and independent analyses, the NRC staff concludes that ABB-CE`s application for design certification meets the requirements of Subpart B of 10 CFR Part 52 that are applicable and technically relevant to the System 80+ standard design. This document, Volume 1, contains Chapters 1 through 14 of this report.

  4. Daimler process audit preparation, execution and evaluation in Faurecia Kosice

    Directory of Open Access Journals (Sweden)

    Ľubomír Lengyel

    2011-04-01

    Full Text Available This paper is describing process of preparation to successful passing of Daimler Process Audit. From process point of view VDA 6.3 standard has been the base accompanied with Daimler additions, driven by internal Faurecia Audit standard. Continual improvement process based on Deming’s cycle has been applied. The paper is intended for all professionals who are concerned with the process and product quality improvement and quality audit in the organization. The paper has been compiled in connection with resolving project KEGA 3/6411/08 Transformation of the already existing study programme Management of production quality to an university-wide bilingual study programme.

  5. Preparation of Gas Separation Membranes and their Evaluation

    Directory of Open Access Journals (Sweden)

    R. S. Chauhan

    2004-07-01

    Full Text Available Asymmetric membranes have been prepared form the polysulfone using dry-wet phase-inversion technique. These membranes show a thin dense skin at one surface and porous as well as the selectivity of membranes. In addition an online permeate composition analysis system has also been setup to quantify the concentration fo the two major components of atmospheric ari, namely oxygen and nitrogen using a gas chromatograph. Memvranes have been tested at various feed pressures. Teh developed membranes are capable of enriching atmospheric air to a level of 30 per cent and more.

  6. Standard practice for preparing sulfur prints for macrostructural evaluation

    CERN Document Server

    American Society for Testing and Materials. Philadelphia

    2008-01-01

    1.1 This practice provides information required to prepare sulfur prints (also referred to as Baumann Prints) of most ferrous alloys to reveal the distribution of sulfide inclusions. 1.2 The sulfur print reveals the distribution of sulfides in steels with bulk sulfur contents between about 0.010 and 0.40 weight percent. 1.3 Certain steels contain complex sulfides that do not respond to the test solutions, for example, steels containing titanium sulfides or chromium sulfides. 1.4 The sulfur print test is a qualitative test. The density of the print image should not be used to assess the sulfur content of a steel. Under carefully controlled conditions, it is possible to compare print image intensities if the images are formed only by manganese sulfides. 1.5 The sulfur print image will reveal details of the solidification pattern or metal flow from hot or cold working on appropriately chosen and prepared test specimens. 1.6 This practice does not address acceptance criteria based on the use of the method. ...

  7. Preparation, Characterization and Evaluation of Elvitegravir-Loaded ...

    African Journals Online (AJOL)

    EVG) by formulating the drug as ... International Pharmaceutical Abstract, Chemical Abstracts, Embase, Index Copernicus, EBSCO, African ... by dynamic light scattering (DLS) principle .... Table 1: Kinetic models for dissolution profile evaluation.

  8. HISTOLOGICAL PREPARATION OF INVERTEBRATES FOR EVALUATING CONTAMINANT EFFECTS

    Science.gov (United States)

    Although many studies in toxicologic pathology evaluate the effects of toxicants on fishes because of their similarities with other vertebrates, invertebrates can also provide insights into toxicant impacts on ecosystems. Invertebrates not only serve as food resources (e.g., ...

  9. HISTOLOGICAL PREPARATION OF INVERTEBRATES FOR EVALUATING CONTAMINANT EFFECTS

    Science.gov (United States)

    Although many studies in toxicologic pathology evaluate the effects of toxicants on fishes because of their similarities with other vertebrates, invertebrates can also provide insights into toxicant impacts on ecosystems. Invertebrates not only serve as food resources (e.g., ...

  10. Certification of ICTs in Elections

    DEFF Research Database (Denmark)

    Schürmann, Carsten; Barrat, Jordi; Bolo, Eden

    2015-01-01

    voting or tabulation systems. The key elements of quality control are certification and evaluation. Certification refers to the confirmation proof of compliance with a given standard. Evaluation is the most labour intensive part of the quality control process during which the requirements, designs......, hardware, firmware, software, networks and operational contexts are examined for faults. An election technology can only be certified by third-party reviewers who are accredited to assess compliance with a standard. An EMB should consider quality control early in the process of introducing new technologies......, starting during the feasibility study, and especially if it is bound by law to provide such a certification. The evaluation reports and related documents can also be used to increase the transparency of the election, improve the dialogue between EMBs and voters, and increase the EMB’s credibility....

  11. Program Evaluation of the "PREPaRE" School Crisis Prevention and Intervention Training Curriculum

    Science.gov (United States)

    Nickerson, Amanda B.; Serwacki, Michelle L.; Brock, Stephen E.; Savage, Todd A.; Woitaszewski, Scott A.; Louvar Reeves, Melissa A.

    2014-01-01

    This study details a program evaluation of the "PREPaRE School Crisis Prevention and Intervention Training Curriculum" ("PREPaRE"), conducted in the United States and Canada between 2009 and 2011. Significant improvements in crisis prevention and intervention attitudes and knowledge were shown among 875 "Crisis Prevention…

  12. Preparation and Evaluation of Emamectin Benzoate Solid Microemulsion

    Directory of Open Access Journals (Sweden)

    Lei Feng

    2016-01-01

    Full Text Available The solid microemulsions of emamectin benzoate with the same content of surfactants were prepared by a self-emulsifying method. Emulsifier 600# and emulsifier 700# (3/2, w/w screened from eleven kinds of commonly used surfactants displayed great emulsifying properties. The redispersed solution of the solid microemulsion presented aqueous microemulsion characteristic. The mean particle size and polydispersity index were 10.34 ± 0.10 nm and 0.283 ± 0.013, respectively. The solid microemulsion showed excellent storage stability and the bioassay compared with water dispersible granules against diamondback moths provided a proof of its improved biological activities. This formulation could significantly reduce surfactants and is perspective in plant protection for improving bioavailability and environmental friendliness.

  13. Preparation and evaluation of Au nanoparticle–silica aerogel nanocomposite

    Directory of Open Access Journals (Sweden)

    Naruhito Katagiri

    2015-06-01

    Full Text Available A Au nanoparticle–silica aerogel nanocomposite was prepared by the gelling of a tetramethyl orthosilicate (TMOS/ethanol solution together with Au nanoparticles and drying the wet gel in supercritical carbon dioxide. The aerogel nanocomposite contained Au nanoparticles at a concentration of 0.268 ppm. The bulk density, porosity, and specific surface area of the obtained nanocomposite were 0.126 g/cm3, 94%, and 890 m2/g, respectively. The nanocomposite was reddish-violet in color and transparent, and had a relative permittivity of approximately 6 with a dielectric loss of ca. 1 × 10−3 in the range of 10 MHz to 1 GHz.

  14. Preparation and in vitro evaluation of tacrolimus-loaded ethosomes.

    Science.gov (United States)

    Li, Guiling; Fan, Chao; Li, Xinru; Fan, Yating; Wang, Xiaoning; Li, Mei; Liu, Yan

    2012-01-01

    The main objective of the present work was to prepare and assess dermal delivery of tacrolimus-loaded ethosomes versus classic liposomes. Both delivery systems were characterized for particle size, polydispersity index, and entrapment efficiency (EE), by dynamic laser diffraction and ultrafiltration or dialysis methods, respectively. The results indicated that presence of ethanol in the formulations affected the particle size. In addition, ultrafiltration method was selected to determine EE due to relatively short period as compared with dialysis method. Ethosomes exhibited a significant higher EE and amount of drug in dermis in contrast to classic liposomes suggesting that ethosomes with higher entrapment capacity prompted more amount of tacrolimus to permeate through stratum corneum and reach the target of atopic dermatitis (AD). Physical stability was very well for tacrolimus-loaded ethosomes under storage condition (4 °C). Our results demonstrated that the ethosomal system might be a promising candidate for dermal delivery of tacrolimus for AD.

  15. Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes

    Directory of Open Access Journals (Sweden)

    Guiling Li

    2012-01-01

    Full Text Available The main objective of the present work was to prepare and assess dermal delivery of tacrolimus-loaded ethosomes versus classic liposomes. Both delivery systems were characterized for particle size, polydispersity index, and entrapment efficiency (EE, by dynamic laser diffraction and ultrafiltration or dialysis methods, respectively. The results indicated that presence of ethanol in the formulations affected the particle size. In addition, ultrafiltration method was selected to determine EE due to relatively short period as compared with dialysis method. Ethosomes exhibited a significant higher EE and amount of drug in dermis in contrast to classic liposomes suggesting that ethosomes with higher entrapment capacity prompted more amount of tacrolimus to permeate through stratum corneum and reach the target of atopic dermatitis (AD. Physical stability was very well for tacrolimus-loaded ethosomes under storage condition (4°C. Our results demonstrated that the ethosomal system might be a promising candidate for dermal delivery of tacrolimus for AD.

  16. Comparative Evaluation Of Topical Preparations In Treatment of Melasma

    Directory of Open Access Journals (Sweden)

    Pandhi Roma

    2002-01-01

    Full Text Available Fifty patients of melasma were randomly taken up for the study and were divided into five treatment groups of 10 patients each. Patients of these groups received clobetasol propionate 0.05% (group I, tretinoin 0.025% (group II, hydroquinone 2% (group III, bebzoyl peroxide 5% (group IV and glycolic acid 10% (group V. Pretreatment evolution was done with MASI (melasma area severity index scoring and photographic records. Patients were treated for 3 months and the preparations were found to be equally compatible to our patients. However, effectiveness on an average was higher for clobetasol (80% and tretinoin (62%, though hydroquinone (48%, benzoyal peroxide (41% and glycolic acid (30% also showed fairly good results. Our study broadens the therapeutic spectrum of melasm by using agents with different mechanisms of action.

  17. No certificate, no chocolate

    CERN Multimedia

    Computer Security Team

    2013-01-01

    Are you already ready to use “certificates” to log into CERN or to connect to the global “eduroam” wireless network? No, I am not talking about your birth certificate, medical certificates or academic certificates. I am referring to “certificates” used for authentication where you would usually use a password.   These digital certificates are a valid alternative to cumbersome passwords. Like the aforementioned personal certificates, a digital certificate is an official document that proves who you are or your qualifications. Your personal digital CERN certificate is tied to your digital identity at CERN. In that respect, a digital certificate is like a password. It is a credential that you must not share with anybody else! With your digital certificate, I can impersonate you and take over your mailbox, your web sessions and more… Digital certificates bind your digital identity to a public/private-key infrastructu...

  18. Preparation and preliminary evaluation of [55Co](Ⅱ)vancomycin

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Co-55 (t1/2=17.53 h) was produced by 150 μA irradiation of a natural nickel target using 15 MeV protons. It was separated from the irradiated target material by two ion exchange chromatography steps with a radiochemical yield of>95% and was used for the preparation of [55Co]vancomycin ([55Co]VAN). Optimization studies were per-formed using Co-57 due to its longer half-life. Cobalt-57 (t1/2=271.79 d) was produced by irradiation of a natural nickel target with 150 μA current of 22 MeV protons. The 57Co was separated from the irradiated target material using a no-carrier-added method with a radiochemical yield of>97%. Both products were controlled for radionuelide and chemical purity. The solutions of [55Co]VAN were prepared (radiochemical yield>80%) starting with 55Co acetate and vancomycin at room temperature after 30 min. A precise solid phrase extraction (SPE) method was developed using Si Sep-Pak in order to purify/reconstitute the final formulation for animal studies. [55Co]VAN showed a radiochemical purity of more than 99%. The resultant specific activity was about 1.15 TBq/mmol. It is proved that the tracer is stable in the final product and in presence of human serum at 37℃ up to 24 h. Biodistribution study of [55Co]VAN in normal rats was undertaken for up to 72 h.

  19. PMP Certification All-In-One Desk Reference For Dummies

    CERN Document Server

    Stackpole, Cynthia Snyder

    2011-01-01

    Be as prepared as possible to take the PMP certification exam The PMP certification is the most popular project management certification available, but also a very difficult certification to obtain with very demanding requirements. That's where this All-in-One reference comes in. Packed with valuable information for taking the exam, the nine books in one covers everything from the certification process to gathering information for the application and signing up to take the exam, as well as studying for the most pertinent parts of the Project Management Body of Knowledge (PMBOK), and review que

  20. Mental Preparation and Evaluation: A Sportpsychological Project with the Swiss Orienteering National Team.

    Science.gov (United States)

    Venzl, Reto

    1994-01-01

    Lists the training themes and levels of intervention of a psychological orienteering project for Swiss athletes. Presents an outline for preparation and evaluation of team or individual performance over time on technical, physical, mental, and environmental aspects of orienteering. (SV)

  1. AN EVALUATIVE STUDY OF AN ICT MODULE FOR A SCHOOL LEADERSHIP AND MANAGEMENT PREPARATION PROGRAM

    Directory of Open Access Journals (Sweden)

    Sarietjie MUSGRAVE

    2017-04-01

    Full Text Available This study reports on findings of an evaluative study on the effectiveness of an information and communication technology (ICT module that forms part of the Advanced Certificate in Education: School Leadership and Management program. The study was carried out among distance education (DE students from the University of the Free State enrolled for above mentioned module. Two ICT audit surveys were used to gather data. This study used two modes of evaluation: Jung and Latchem’s (2007 quality indicators for DE and comparison. The findings highlight ready access to ICT, technical support, appropriate guidance and support by knowledgeable, innovative and committed facilitators and the creation of a sense of community as imperatives for teaching education leaders ICT skills and knowledge.

  2. Western Kentucky University Teacher Preparation Evaluation System. Case Study.

    Science.gov (United States)

    Western Kentucky Univ., Bowling Green.

    Western Kentucky University is engaged in testing an evaluation system designed to obtain objective, quantifiable data on graduates of its teacher education program. Each year 20 elementary and 20 secondary participants are randomly selected at the beginning of their student teaching experience. Participants are observed during their preservice…

  3. PREPARATION AND CERTIFICATION OF NEW CRM OF MAGNESIUM ALLOY Mg-Al-Zn-Y FOR SPECTRAL ANALYSIS%新型镁合金Mg-Al-Zn-Y光谱标准样品的制备和定值

    Institute of Scientific and Technical Information of China (English)

    朱学纯; 刘功达

    2011-01-01

    New CRM of magnesium alloy Mg-Al-Zn-Y for spectral analysis was developed and prepared. During itspreparation, the technique "Double Layer Safety" was employed to melt magnesium, and "All Around Inner Wall Deslagging Method" to remove the oxide skin and slag in the mold. The stability and uniformity of the CRM were investigated and the period of validity of the CRM was 5 years. The newly updated software "Data-Processing System (StndMtrl) " was employed to test and compute the original data from cooperation certificating of several laboratories and then the standard certification and deviation were obtained. The prepared CRM was anodized to keep it from surface oxidation corrosion.%采用双层安全炉熔炼镁合金和环壁式除渣法清除结晶器中的氧化皮和夹渣等技术,研制了新型镁合金Mg-Al-Zn-Y光谱标准样品.考察了标准样品的均匀性和稳定性,该标准样品有效期为5年.使用先进的数据处理软件(StndMtrl),对多家单位协作分析定值原始数据进行检验和计算,得到标准样品的标准值和标准偏差.用化学法对标准样品进行氧化上色,防止表面氧化腐蚀.

  4. Preparation and evaluation of some amide ether carboxylate surfactants

    Directory of Open Access Journals (Sweden)

    M.M.A. El-Sukkary

    2012-06-01

    Full Text Available A homologous series of new mild surfactants, namely: Alkyl amide ether carboxylates surfactants (AEC RCO–NHCH2CH2O (CH2CH2O6CH2COONa, were synthesized by esterification, amidation, ethoxylation and carboxymethylation reaction steps of fatty acids (Lauric, Myristic, palmitic, stearic, oleic or linoleic. The chemical structures of the prepared compounds were confirmed using different spectroscopic techniques, FTIR spectroscopy, mass spectra and HNMR. The surface properties including surface and interfacial tensions, foaming height, emulsification power, calcium ion stability, stability to hydrolysis and critical micelle concentration (cmc were determined. The study of their surface properties showed their stability in hard water and in acidic and alkaline media. These compounds have high calcium ion stability. The low foaming power could have an application in the dyeing auxiliary industry. The lower values of the interfacial tension values indicate the ability of using these surfactants in several applications as corrosion inhibitors and biocides. The data revealed various advantages and potentials as a main surfactant as well as co- surfactants.

  5. EVALUATION OF STANDARDS OF SOME SELECTED COSMETIC PREPARATIONS

    Directory of Open Access Journals (Sweden)

    T. AKELESH, R. SIVA KUMAR, R. JOTHI VIJAI RAJAN, P. ARULRAJ,R.VENKATNARAYANAN

    2013-10-01

    Full Text Available The aim of the present work is to analyze the standards of marketed cosmetic products which are largely consumed in day to day life of the people. The cosmeceutical should be tested for efficacy to ensure a proven skin benefit and also to substantiate marketing claims. The work was done by keeping the ideas of Bureau of Indian Standards to analyze the cosmetic products. The evaluation for the following cosmetics such as tooth pastes (Colgate, Closeup, Pepsodent, Vicco and Anchor and face powders (Ponds, Eva, Fa, Cuticura and Spinz are performed for their quality. All the marketed tooth pastes and face powders which had been evaluated complied with the standards specified by Bureau of Indian Standards. Hence all the selected marketed tooth pastes and selected face powders were found to be of good quality.

  6. Preparation and evaluation of diclofenac sodium orally disintegrating tablets

    Directory of Open Access Journals (Sweden)

    Iancu Valeriu

    2016-06-01

    Full Text Available Orally disintegrating tablets (ODTs are dosage forms which disintegrate in mouth within seconds without need of water. This type of quality in dosage form can be attained by addition of different varieties of excipients. Pharmaburst™ 500 is a co-processed excipient system which allows rapid disintegration and low adhesion to punches. The aim of the present study was to develop and evaluate 25 mg diclofenac sodium ODTs (orodispersible tablets batches by direct compression method at different compression forces 10 kN (F1 and 20 kN (F2 and directly compressible excipients used in different ratio (Avicel PH 102, magnesium stearate and coprocessed excipient Pharmaburst™ 500, 70% and 80% w/w. The obtained batches were analyzed for appearance, tablet thickness, uniformity of weight, hardness, friability, disintegration time, and non-compendial methods (wetting time. Co-processed Pharmaburst™ 500 excipient 70% used for sodium diclofenac ODT obtaining determined good results for quality control tests evaluation.

  7. Increasing certification through unit-based education.

    Science.gov (United States)

    Fischer-Cartlidge, Erica; Mahon, Suzanne

    2014-04-01

    Certification has been identified by multiple organizations as an important component and means of elevating the level of nursing care provided to patients and demonstrating to the public that the nursing staff has subspecialty knowledge. Certification may lead to improved patient satisfaction and outcomes as well as increased nurse satisfaction and retention. Despite the known potential benefits associated with certification, institutions struggle to improve certification rates. One possible method to overcome system barriers to certification is the implementation of a unit-based study course to prepare nurses for the Certified Breast Care Nurse (CBCN) examination. Data collected by an author-developed tool as one institution created and executed a unit-based study course suggest that such an approach increased certification rates and improved disease-specific knowledge and confidence among the staff, despite no official data existing on the tool's reliability and validity. Implementation of similar programs may be successful in improving certification at other institutions seeking to raise certification rates.

  8. Preparation and evaluation of appertized from snail Helix aspersa M

    Directory of Open Access Journals (Sweden)

    Nelson Loyola López

    2015-01-01

    Full Text Available This study includes the development and evaluation of snails (Helix aspersa M. appertized, collected at a heliciculture breeding center, located in Los Niches sector, Curico, Maule region, South-central of Chile. The test was conducted at the Laboratory of Sciences of the Catholic University of Maule, Nuestra Señora del Carmen Campus, Curico. The main objective of this work was to study the influence of appertized on sensory attributes and commercial durability of snail Helix aspersa M. Additionally, some specific objectives were proposed as follow: to provide this mollusc with a commercial alternative for it consume, to evaluate its organoleptic characteristics and guarantee the product from both the microbiological and nutritional points of view. Three media cover were used (T0: water + NaCl 2%; T1: Water + NaCl 2% + citric acid 0.5% + kilol and T2: extra virgin olive oil + spices + tocopherol. The product was assessed at two different times, after 30 and 90 days of storage. Two sensory evaluations were conducted to measure various organoleptic attributes and acceptability of the appertized by 14 trained panelists. Amino acid, vitamins, cholesterol, acidity, heavy metals, phosphorus and organochlorines analysis were performed. The presence of both total and fecal contaminant microorganisms was determined. Attributes such as color, flavor, aroma, texture and overall acceptability were also measured. Preserves made by T0 and T1 treatments were equally accepted by the panelists. However, preserve from treatment T2 was rejected because of the detection in them of a very dark color, odor and mealy texture. Positive results regarding the content of amino acids, vitamin C and low cholesterol, as well as the absence of pathogenic microorganisms were obtained for the three treatments.

  9. Evaluation and Certification of Ambersorb 4652 for use in Activated Carbon Ion Exchange Filters for the International Space Station

    Science.gov (United States)

    Adam, Niklas; Cox, Trey; Larner, Katherine; Carter, Donald; Kouba, Coy

    2017-01-01

    In order to reduce the infiltration of dimethylsilanediol (DMSD) and other organosilicon containing species through the Multifiltration Beds (MF Beds), an alternate activated carbon was found to replace the obsolete Barnabey Cheney 580-26 activated carbon. The carbon that removed the most organosilicon compounds in testing1 was a synthetic activated carbon named Schunk 4652 which later became Ambersorb 4652. Since activated carbon has a large capacity for iodine (I2), and is used in the Activated Carbon Ion Exchange (ACTEX) filters on the International Space Station (ISS), testing was performed on the Ambersorb 4652 carbon to determine the effectiveness of the material for use in ACTEX filters to remove iodine. This work summarizes the testing and the certification of Ambersorb 4652 for use in the ACTEX filters for the ISS.

  10. Software Certification and Software Certificate Management Systems

    Science.gov (United States)

    Denney, Ewen; Fischer, Bernd

    2005-01-01

    Incremental certification and re-certification of code as it is developed and modified is a prerequisite for applying modem, evolutionary development processes, which are especially relevant for NASA. For example, the Columbia Accident Investigation Board (CAIB) report 121 concluded there is "the need for improved and uniform statistical sampling, audit, and certification processes". Also, re-certification time has been a limiting factor in making changes to Space Shuttle code close to launch time. This is likely to be an even bigger problem with the rapid turnaround required in developing NASA s replacement for the Space Shuttle, the Crew Exploration Vehicle (CEV). Hence, intelligent development processes are needed which place certification at the center of development. If certification tools provide useful information, such as estimated time and effort, they are more likely to be adopted. The ultimate impact of such a tool will be reduced effort and increased reliability.

  11. Evaluation of ultrasonic and ErCr:YSGG laser retrograde cavity preparation.

    Science.gov (United States)

    Batista de Faria-Junior, Norberto; Tanomaru-Filho, Mário; Guerreiro-Tanomaru, Juliane Maria; de Toledo Leonardo, Renato; Camargo Villela Berbert, Fábio Luiz

    2009-05-01

    Root end cavity preparation techniques aim to create a clean and properly shaped cavity in a short time. Although the use of ultrasonics has been widely recommended, a laser can also be used. This study evaluated the time required and quality of retrograde cavity preparations using ultrasonics or ErCr:YSGG laser. Thirty single-rooted teeth were instrumented, root filled, submitted to apicectomies, and grouped. Root end cavities were prepared by using the following: group 1 (G1): CVD (6.1107-6) ultrasonic retrotips (CVD-Vale, São José dos Campos, Brazil); group 2 (G2): EMS (DT-060/Berutti) ultrasonic retrotips (EMS, LeSentier, Switzerland); and group 3 (G3): ErCr:YSGG (G6/Waterlase; Biolase Technology, San Clemente, CA) laser tips. The time taken to complete the preparation was recorded. Epoxy resin replicas of the root apices were examined under a scanning electron microscope. The parameters for evaluation were the presence of fractures, and the quality of the preparations. The Waterlase showed the highest mean time for preparation of the root end cavities (p 0.05). Fractures in the cavosurface angle occurred only in G2. G1 and G2 showed better scores for quality of preparation than G3 (p < 0.05). These results suggest that root end cavities should be prepared by ultrasonic tips.

  12. Students’ evaluation of preclinical simulation for all ceramic preparation (In Faculty of Dentistry Universiti Kebangsaan Malaysia

    Directory of Open Access Journals (Sweden)

    Natasya Ahmad Tarib

    2008-06-01

    Full Text Available The purpose of the study is to evaluate all ceramic crown (ACC preparations those were made by dental undergraduate students during the preclinical sessions. 104 plastic teeth were prepared by 4th year dental undergraduates during the preclinical session for ACC crown examined. The teeth were placed on the frasaco arches and were mounted in the frasaco head. The preparations were examined for the tapering, presence of undercuts, incisal and cingulum reductions as well as preparation of shoulder margin. Preparations were examined using hand instruments and visual. The sample size was 92 plastic teeth. Most of the preparations were acceptable with acceptable placement and types of margins, adequate axial and incisal reductions and acceptable tapered of the axial walls. On the other hand, most of the teeth showed absence of cingulum wall. Most of the crowns prepared by the students were acceptable. It showed that they understood the principles of crown preparation. Cingulum wall preparation has to be given greater emphasis as it is important in the retention and resistance of the restoration.

  13. Preparation and evaluation of dispersible tablets of Trikatu extract

    Directory of Open Access Journals (Sweden)

    Munish Garg

    2014-01-01

    Full Text Available Traditional Ayurvedic formulations are consumed increasingly as dietary supplements to fight or prevent various types of diseases since ages. "Tlikatu" (Sanskrit, meaning "three acrid", is a mixture of black pepper (Piper nigrum; long pepper (Piper longum; and ginger (Zingiber ofticinale; widely used in Ayurvedic system of medicines to enhance bioavailability. However being consumed in powder form, it is associated with certain limitations of large and uncalculated dose and uncertain quality. To overcome this, the present study was carried out to develop standardized dispersible tablets of Trikatu extracts. Three main ingredients were thus subjected for extraction, screened for phytochemical status using standard markers by high-performance thin-layer chromatographyhigh-performance thin-layer chromatography and mixed proportionately. A method was then developed to formulate these extracts into dispersible tablets by direct compression method and evaluated for their physico-chemical properties, which have demonstrated good flow properties with zero drug-excipient incompatibility, less disintegration time and other requirements within pharmacopoeial limits. This scientifically developed formulation intends to have advantages over the traditional form and thus be able to deliver better therapeutic results.

  14. Preparation and Lab Evaluation of a New Denture Adhesive

    Institute of Scientific and Technical Information of China (English)

    ZHAO Ke; TIAN Tian; ZHU Weniun; YU Suihua

    2011-01-01

    To develop a new kind of denture adhesive (DA),then to evaluate its mechanical and chemical properties,polyethylene oxide,methyl cellulose,sodium alginate,and cellulose ether,were viewed as four main factors,which would affect bonding load of DA,while two levels of each component were differentiated.Following table L12(211),twelve different formulae were designed by orthogonal design and bonding load values were recorded.According to bonding load of each formula,the optimized formula was selected out as this new adhesive material,Comfort-DA Ⅱ.The changing of bonding load values of Comfort-DA Ⅱ marinating in artificial saliva were measured,and an existing product,Protefix and Comfort-DA,were used as controls.Following initial load readings,samples and controls were measured from 1- to 12- hour intervals,respectively.Comfort-DA Ⅱ was diluted at 1.0%,2.0%,3.3%,5.0%,and 10.0% concentrations,and pH values were measured at 1-,2-,3-,4-,5-,6-,and 8-hour intervals.Comfort-DA Ⅱ presented as a pale-yellow paste.Group 6 gained the highest bonding load value of [193.8 (4.2)]N.The mean bonding load of Comfort-DA Ⅱ was statistically different to Comfort-DA (p =0.004) and Protefix (p =0.006).Comfort-DA Ⅱ exhibited a progressive increase in pH value over time and was slightly alkaline.Comfort-DA Ⅱ showed significantly elevated mechanical and chemical properties,comparing to Comfort-DA.The results also indicate that orthogonal design may be an efficacious way to develop new dental materials.

  15. PKIX Certificate Status in Hybrid MANETs

    Science.gov (United States)

    Muñoz, Jose L.; Esparza, Oscar; Gañán, Carlos; Parra-Arnau, Javier

    Certificate status validation is a hard problem in general but it is particularly complex in Mobile Ad-hoc Networks (MANETs) because we require solutions to manage both the lack of fixed infrastructure inside the MANET and the possible absence of connectivity to trusted authorities when the certification validation has to be performed. In this sense, certificate acquisition is usually assumed as an initialization phase. However, certificate validation is a critical operation since the node needs to check the validity of certificates in real-time, that is, when a particular certificate is going to be used. In such MANET environments, it may happen that the node is placed in a part of the network that is disconnected from the source of status data at the moment the status checking is required. Proposals in the literature suggest the use of caching mechanisms so that the node itself or a neighbour node has some status checking material (typically on-line status responses or lists of revoked certificates). However, to the best of our knowledge the only criterion to evaluate the cached (obsolete) material is the time. In this paper, we analyse how to deploy a certificate status checking PKI service for hybrid MANET and we propose a new criterion based on risk to evaluate cached status data that is much more appropriate and absolute than time because it takes into account the revocation process.

  16. Renewable Energy Certificate Program

    Energy Technology Data Exchange (ETDEWEB)

    Gwendolyn S. Andersen

    2012-07-17

    This project was primarily to develop and implement a curriculum which will train undergraduate and graduate students at the University seeking a degree as well as training for enrollees in a special certification program to prepare individuals to be employed in a broad range of occupations in the field of renewable energy and energy conservation. Curriculum development was by teams of Saint Francis University Faculty in the Business Administration and Science Departments and industry experts. Students seeking undergraduate and graduate degrees are able to enroll in courses offered within these departments which will combine theory and hands-on training in the various elements of wind power development. For example, the business department curriculum areas include economic modeling, finance, contracting, etc. The science areas include meteorology, energy conversion and projection, species identification, habitat protection, field data collection and analysis, etc.

  17. A New Approach to Educator Preparation Evaluation: Evidence for Continuous Improvement?

    Directory of Open Access Journals (Sweden)

    Corinne Baron Donovan

    2014-05-01

    Full Text Available The landscape for educator preparation has shifted to accountability models emphasizing performance assessment of teaching, employer feedback reports, newly approved accreditation standards showing impact on K-12 student learning, and expectations of public access to all of this information. This article provides a perspective on the extent to which this change offers promise for improving educator preparation programs and consequently excellence in teaching in K-12 schools. Two accountability reports are used as the empirical evidence for review; one is a pilot institutional feedback report from the Teacher Quality Research Center (Boyd, Lankford, & Wyckoff, 2009 and the second is a new Teacher Preparation Program report prepared by New York City’s department of education (NYCDOE, 2013. Ultimately, a systems perspective is recommended, in which candidates, IHEs, and K-12 schools are involved in the process of how educator preparation is evaluated and how that connects to other aspects of the education profession.

  18. Evaluation of seamless ligation cloning extract preparation methods from an Escherichia coli laboratory strain.

    Science.gov (United States)

    Okegawa, Yuki; Motohashi, Ken

    2015-10-01

    Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA cloning without the use of restriction enzymes. Instead, SLiCE uses homologous recombination activities from Escherichia coli cell lysates. To date, SLiCE preparation has been performed using an expensive commercially available lytic reagent. To expand the utility of the SLiCE method, we evaluated different methods for SLiCE preparation that avoid using this reagent. Consequently, cell extracts prepared with buffers containing Triton X-100, which is a common and low-cost nonionic detergent, exhibited sufficient cloning activity for seamless gene incorporation into a vector.

  19. EVALUATION OF OIL BASED AVIAN INFLUENZA VACCINE (H5NI PREPARED WITH DIFFERENT CONCENTRATIONS OF ADJUVANT

    Directory of Open Access Journals (Sweden)

    M. IQBAL, M. NISAR, ANWARUL-HAQ, S. NOOR AND Z. J. GILL

    2008-12-01

    Full Text Available Bird flu vaccine from H5N1 strain of avian influenza virus was prepared with two concentrations of adjuvant (Montanide ISA 70MVG. Two vaccines (I and II were prepared containing 50 and 60% Montanide, respectively. Immune response of both the vaccines as single, as well as booster, dose was evaluated in layer birds through haemagglutination inhibition test. Single dose of both vaccines showed poor immune response, while booster dose gave better response with both the vaccines. However, the vaccine prepared with 60% Montanide provided better immune response compared with the vaccine containing 50% montanide.

  20. AEG评审在型号合格审定中的研究与应用%Research and Application of AEG Evaluation on Type Certification

    Institute of Scientific and Technical Information of China (English)

    段本印

    2015-01-01

    概述了申请人的航空器评审工作,并简要对比分析了运行符合性清单、型别等级和训练要求、MMEL、MRBR、OCAI等11个AEG工作评审项目。同时通过对比分析航空器评审组(简称AEG)评审与型号合格审定之间的区别和联系,为申请人顺利开展AEG工作提出4条建议。%The applicant’ s work of aircraft evaluation is introduced in this paper, and 11 aircraft evaluation items are listed, including operation compliance checklist, type rating and qualification specification, MMEL, MRBR, OCAI, etc. The difference and relation between aircraft evaluation and type certification are contrasted and ana-lyzed at the same time ,and 4 suggestions are provided to the applicant in order to develop aircraft evaluation.

  1. 45 CFR 1301.13 - Accounting system certification.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Accounting system certification. 1301.13 Section... START PROGRAM HEAD START GRANTS ADMINISTRATION General Requirements § 1301.13 Accounting system... shall submit an accounting system certification, prepared by an independent auditor, stating that...

  2. Airframe and Powerplant Mechanics Certification Guide. Revised 1971.

    Science.gov (United States)

    Federal Aviation Administration (DOT), Washington, DC. Flight Standards Service.

    The guide was prepared to provide information to prospective airframe and powerplant mechanics and other persons interested in the certification of mechanics. The requirements for a mechanic certificate are concerned with age, language ability, experience, knowledge, and skill. The sections of the guide explain the procedure for either…

  3. Impact of Digital Tooth Preparation Evaluation Technology on Preclinical Dental Students' Technical and Self-Evaluation Skills.

    Science.gov (United States)

    Gratton, David G; Kwon, So Ran; Blanchette, Derek; Aquilino, Steven A

    2016-01-01

    The aim of this study was to evaluate the effect of digital tooth preparation imaging and evaluation technology on dental students' technical abilities, self-evaluation skills, and the assessment of their simulated clinical work. A total of 80 second-year students at one U.S. dental school were assigned to one of three groups: control (n=40), E4D Compare (n=20), and Sirona prepCheck (n=20). Students in the control group were taught by traditional teaching methodologies, and the technology-assisted groups received both traditional training and supplementary feedback from the corresponding digital system. Three outcomes were measured: faculty technical score, self-evaluation score, and E4D Compare scores at 0.30 mm tolerance. Correlations were determined between the groups' scores from visual assessment and self-evaluation and between the visual assessment and digital scores. The results showed that the visual assessment and self-evaluation scores did not differ among groups (p>0.05). Overall, correlations between visual and digital assessment scores were modest though statistically significant (5% level of significance). These results suggest that the use of digital tooth preparation evaluation technology did not impact the students' prosthodontic technical and self-evaluation skills. Visual scores given by faculty and digital assessment scores correlated moderately in only two instances.

  4. Evaluation of Electrospray as a Sample Preparation Tool for Electron Microscopic Investigations: Toward Quantitative Evaluation of Nanoparticles.

    Science.gov (United States)

    Mielke, Johannes; Dohányosová, Pavla; Müller, Philipp; López-Vidal, Silvia; Hodoroaba, Vasile-Dan

    2017-02-01

    The potential of electrospray deposition, for the controlled preparation of particles for imaging in electron microscopes, is evaluated on various materials: from mono-modal suspensions of spherical particles to multimodal suspensions and to real-world industrial materials. It is shown that agglomeration is reduced substantially on the sample carrier, compared with conventional sample preparation techniques. For the first time, it is possible to assess the number concentration of a tri-modal polystyrene suspension by electron microscopy, due to the high deposition efficiency of the electrospray. We discovered that some suspension stabilizing surfactants form artifact particles during electrospraying. These can be avoided by optimizing the sprayed suspension.

  5. World Record Earned Value Management System Certification for Cleanup of the East Tennessee Technology Park, Oak Ridge, Tennessee, USA - 13181

    Energy Technology Data Exchange (ETDEWEB)

    Haynes, Ray; Hirschy, Anita [URS - CH2M Oak Ridge, LLC (UCOR), East Tennessee Technology Park D and D and Environmental Remediation Project, Oak Ridge, Tennessee 37830 (United States)

    2013-07-01

    and site. We also had corporate EVMS experts available to us so as to draw upon their recent certification experiences with lessons learned. This knowledge was especially helpful for training of personnel that were involved in the certification which included Project Controls, Project Management and Control Account Managers. We were also able to bring in these corporate experts to assist with our training efforts. To assure our readiness for the review, we conducted a 'White Hat' review. The 'White Hat' team consisted of corporate experts in EVMS along with an industry expert in EVMS from Humphrey and Associates. This review identified early any weaknesses that we had so corrections could be enacted prior to the EVMS Certification Readiness Review. It also helped give the evaluators confidence that we had done proper due diligence prior to their arrival. Also critical to our success, was early communication with our evaluators. It is important to start the communications early to ensure you understand the expectations of the certification team and the process that will be used during the certification. Communication through the entire process is critical to understand expectations and issues along the way. Very important to the overall process was management commitment, support and reinforcement. Management made sure that all personnel involved knew the importance and made preparations a priority. This was noted as a key strength by the evaluators during the out-brief. As a result of our preparation, our review yielded one Corrective Action Report (CAR) and two Continuous Improvement Opportunities (CIOs). The Certification team in their out-brief explained that this was the lowest number of CARs and CIOs in the history of EVMS certifications in the DOE Complex. (authors)

  6. Lights, Camera, Action: Advancing Learning, Research, and Program Evaluation through Video Production in Educational Leadership Preparation

    Science.gov (United States)

    Friend, Jennifer; Militello, Matthew

    2015-01-01

    This article analyzes specific uses of digital video production in the field of educational leadership preparation, advancing a three-part framework that includes the use of video in (a) teaching and learning, (b) research methods, and (c) program evaluation and service to the profession. The first category within the framework examines videos…

  7. Western Kentucky University's Teacher Preparation Evaluation Model Phase I, Cycle I. Annual Report.

    Science.gov (United States)

    Adams, Ronald D.

    A teacher preparation evaluation model was developed at Western Kentucky University. A total of 18 secondary and 20 elementary student teachers participated in the study. Instrumentation and records used for data collection consisted of five general types: a questionnaire, a personality scale, rating scales, direct classroom observational systems,…

  8. School Leadership Preparation and Development in Kenya: Evaluating Performance Impact and Return on Leadership Development Investment

    Science.gov (United States)

    Asuga, Gladys; Eacott, Scott; Scevak, Jill

    2015-01-01

    Purpose: The purpose of this paper is to evaluate the quality of the current provision for school leadership in Kenya, the extent to which they have an impact on student outcomes and the return on school leadership preparation and development investment. Design/Methodology/Approach: The paper draws from educational leadership, management and…

  9. Preparation and evaluation of glycosylated arginine-glycine-aspartate (RGD) derivatives for integrin targeting.

    NARCIS (Netherlands)

    Kuijpers, B.H.M.; Groothuys, S.; Soede, A.C.; Laverman, P.; Boerman, O.C.; Delft, F.L. van; Rutjes, F.P.J.T.

    2007-01-01

    Arginine-glycine-aspartate (RGD) derivatives were prepared by a combination of solid-phase and solution-phase synthesis for selective targeting of alpha vbeta 3 integrin expressed in tumors. In order to evaluate the value of a triazole moiety as a proposed amide isostere, the side chain glycosylated

  10. Evaluation of resistance form of different preparation features on mandibular molars

    Directory of Open Access Journals (Sweden)

    Bajoghli Farshad

    2013-01-01

    Full Text Available Aims: Resistance form of full metal-ceramic crown is an important feature that determines longevity of these restorations. This study evaluated the resistance form of full metal-ceramic prepared with four different design features. Materials and Methods: An acrylic tooth was prepared with 20° total occlusal convergence (TOC angle, 2.5 mm of occlusocervical dimension and a shoulder finishing line. This design lacked resistance form. The crown preparation was subsequently modified by preparing Mesial Occlusal Distal isthmus, placing occlusal inclined plane, and reducing TOC. Four metal dies from these designs were constructed by lathe machine and then 10 metal copings were fabricated for each preparation. Metal coping were cemented on metal dies with temp-bond cement. Force was applied at 45° from lingual to buccal direction with universal testing machine. Statistical analysis used: The data were evaluated by Kruskal-Wallis and non-parametric Mann-Whitney test. Results: All features increased resistance form when compare to control group. However, reduce TOC group showed greatest value of resistance. Conclusion: Within the limitation of this study, reducing the tapering of occlusocervival dimension is the most effective way in increasing resistance form, although, other features were also effective.

  11. Reliability of chemotherapy preparation processes: Evaluating independent double-checking and computer-assisted gravimetric control.

    Science.gov (United States)

    Carrez, Laurent; Bouchoud, Lucie; Fleury-Souverain, Sandrine; Combescure, Christophe; Falaschi, Ludivine; Sadeghipour, Farshid; Bonnabry, Pascal

    2017-03-01

    Background and objectives Centralized chemotherapy preparation units have established systematic strategies to avoid errors. Our work aimed to evaluate the accuracy of manual preparations associated with different control methods. Method A simulation study in an operational setting used phenylephrine and lidocaine as markers. Each operator prepared syringes that were controlled using a different method during each of three sessions (no control, visual double-checking, and gravimetric control). Eight reconstitutions and dilutions were prepared in each session, with variable doses and volumes, using different concentrations of stock solutions. Results were analyzed according to qualitative (choice of stock solution) and quantitative criteria (accurate, 30% deviation). Results Eleven operators carried out 19 sessions. No final preparation (n = 438) contained a wrong drug. The protocol involving no control failed to detect 1 of 3 dose errors made and double-checking failed to detect 3 of 7 dose errors. The gravimetric control method detected all 5 out of 5 dose errors. The accuracy of the doses measured was equivalent across the control methods ( p = 0.63 Kruskal-Wallis). The final preparations ranged from 58% to 60% accurate, 25% to 27% weakly accurate, 14% to 17% inaccurate and 0.9% wrong. A high variability was observed between operators. Discussion Gravimetric control was the only method able to detect all dose errors, but it did not improve dose accuracy. A dose accuracy with <5% deviation cannot always be guaranteed using manual production. Automation should be considered in the future.

  12. Preparation and Performance Evaluation of Biochars from Neem Seed Active Substance Extracted Residues (NSASER)

    Science.gov (United States)

    Li, Jialei; Lu, Shuwen

    2017-05-01

    Neem seed active substance extracted residues (NSASER) is industrial by-product, which is often discarded as a waste. It would lead to a certain degree of harm to the environment. The aim of this study was to prepare the biochars with neem seed active substance extracted residues (NSASER) under the anaerobic pyrolysis conditions. The pyrolysis process was studied with different pyrolysis power (200W, 500W, 800W, 1100W and 1400W), and the performance of the prepared biochars was evaluated. The results showed that the required time was to complete the pyrolysis process that gradually decreased with pyrolysis power increased from 200 to 1400 W, and the final pyrolysis temperature was to complete the pyrolysis process that increased with pyrolysis power increased from 200 to 1400 W. The biochars yield decreased with pyrolysis power increased from 200 to 1400 W, and the biochars yield has the maximum value when the pyrolysis power was of 200W. And the prepared biochars still had some characteristics of the plant cell and kept uniform porous structure, which was beneficial to absorb the small molecule substance. The water content of the prepared biochars was 7.18±0.53, the ash content of the prepared biochars was 5.92±0.31 and the fixed carbon content of the prepared biochars was 81.27±0.89. Compared with the bamboo charcoal, the performance index of the prepared biochars was in according with National Standard of the People’s Republic of China GBT26913-2011 of the bamboo charcoal. The prepared biochars had a potential value in application.

  13. Preparation of a cereal bar containing bocaiuva: physical, nutritional, microbiological and sensory evaluation

    Directory of Open Access Journals (Sweden)

    Cláudia Leite Munhoz

    2014-02-01

    Full Text Available Regional fruit have been increasingly used in recent years in the preparation of foodstuffs because besides promoting the biome preservation, it is obtained differentiated and value-added products. This study aimed to prepare cereal bars containing pulp and kernel of bocaiuva and determine the nutritional quality, assess the acceptability, and microbiological quality. Two formulations of cereal bar were prepared with pulp dehydrated by osmoconvection and kernel of bocaiuva. The formulations were analyzed as for the texture, color analysis, proximate composition, fatty acids profile, mineral, microbiology and sensory evaluation. Cereal bars presented on average, in g 100 g-1, 4.83 moisture, 8.01 protein, 12.93 lipids, 1.30 ash, 53.75 total carbohydrate, 19.78 fiber and 363.41 kcal 100 g-1 total caloric value. Bars represented a source of calcium and iron and had a high content of oleic acid, average of 20 g 100 g-1 total lipids. In the microbiological evaluation, cereal bars have met the standards set by the legislation, being suitable for consumption. As for the attributes evaluated in the sensory analysis, all showed mean values above 6, considered acceptable for consumption. The use of bocaiuva may contribute to highlight differentiated taste and appearance, emphasizing the use of native fruits in the preparation of new products.

  14. Preparation and evaluation of cationic bolaform surfactants for water-based drilling

    Directory of Open Access Journals (Sweden)

    M.M. Dardir

    2017-03-01

    Full Text Available Three cationic bolaform surfactants with different spacer lengths were prepared from the reaction of two moles of triisopropanolamine with one mole of each of the following 1,4-dibromobutane, 1,5-dibromopentane and 1,6-dibromohexane. The chemical structures of the prepared compounds were confirmed via: FTIR spectroscopy, 1H NMR and elemental microanalysis. The surface activity of these bolaform surfactants was studied. The prepared cationic bolaform surfactants were evaluated as viscosifier additives for water-based drilling fluids. The evaluation includes the study of rheological properties of the formulated mud (apparent viscosity, plastic viscosity and yield point, gel strength and thixotropy, effect of temperature on the rheological properties and also, the study of mineralogical properties of the water-based before and after treatment with the prepared surfactants using: X-ray diffraction (XRD, scanning electron microscope (SEM and transmission electron microscope (TEM. The results of the evaluation were compared to the water-based mud formulated from commercial grade bentonite.

  15. Preparation and evaluation of abietic acid microcapsules by a solvent evaporation technique.

    Science.gov (United States)

    Puranik, P K; Manekar, N C; Dorle, A K

    1992-01-01

    Abietic acid was isolated from rosin N Grade (ISI) by a simple process and the product was further standardized. Sulphadiazine microcapsules were prepared by the solvent evaporation technique, using abietic acid as a wall-forming material. Discrete, spherical and free-flowing microcapsules were obtained by phase separation induced by solvent evaporation using bentonite as a solid emulsifier. The prepared microcapsules were evaluated for drug content, wall thickness, flow properties, size distribution, density and in vitro dissolution studies in gastric fluid. The effect of various process variables such as agitation speed, coat-core ratio, etc., on the micromeritic and release characteristics has been described.

  16. Evaluation of the Impact of Obtaining ISO 9001:2008 Quality Management System(QMS Certification by Manufacturing Companies in Zimbabwe.

    Directory of Open Access Journals (Sweden)

    TAUYANASHE CHIKUKU

    2012-09-01

    Full Text Available Getting ISO certification is widely touted as a major boost to a company’s performance, but do the results on the ground show this to be necessarily true? Many executives from certified companies would want an answer to the question ‘Was it worth it?’ and those from non-certified companies would want to answer the question ‘Is itworth it?’ Both groups would also desire to know the best method of determining the answers to these questions. Hence they desire to be furnished with a good performance measurement system (PMS. This paper will give results of the survey of the impact using the framework to evaluate the performance of a number of ISO 9001:2008 certified companies in Zimbabwe. An analysis of the results will be done and recommendation on changes and improvements needed by the firms if they are to fully benefit from the ISO 9001 quality systemwill be made basing on the outcome and also benchmarking.

  17. [Quality evaluation of the orthodontic practice for certification by ISO 9001. A procedure beneficial for medical, medico-dental or hospital service].

    Science.gov (United States)

    Becker, G

    2001-01-01

    The accreditation of the ISO 9001 certification (ISO = International Standard Organization) is an external evaluation procedure carried out by independent experts, whose object is the analysis of the operational methods and practices of a medical care facility (e.g. hospital, private clinic, general practitioner's or dentist's practice) which decided to assume the concept, implementation and control of its own quality policy. The whole accreditation procedure represents the basic structure of a continuous dynamic progressiveness within a cabinet eager to offer outstanding quality. Moreover, it guarantees active and voluntary participation of every single member of the medical administration or technical team involved in the realization of this primary objective. In other words, we are talking about a very strong dynamic innovation leading to a change of views and the improvement of communication means, while simultaneously enhancing the security and quality aspects of medical care. The continuous guarantee of high quality medical care calls for precise planning and systematization of actions. First of all, these actions are defined, analyzed and listed in precise work procedures. As they are defined with the agreement of the whole team, they implicate respect and self control. This requires of course transparency of the treatment methods, whose different steps and procedures are described in detail in a logogramm set up in common.

  18. Development of olmesartan medoxomil lipid-based nanoparticles and nanosuspension: preparation, characterization and comparative pharmacokinetic evaluation.

    Science.gov (United States)

    B, Arun; D, Narendar; Veerabrahma, Kishan

    2017-03-14

    The aim was to enhance the oral bioavailability of olmesartan medoxomil (OM) by preparing solid lipid nanoparticles (SLNs) and comparing with nanosuspension (OM-NS). OM-SLNs and OM-NS were prepared by known methods. Prepared SLNs were evaluated for physical characters and in vivo pharmacokinetic (PK) performance in rats. OM-NS showed more than four-fold increase in the solubility. During DSC and XRD studies, drug incorporated in SLNs was found to be in amorphous form. The relative bioavailability of OM-SLN and OM-NS was 7.21- and 3.52-fold when compared with that of coarse suspension. Further, OM-SLNs also increased the oral bioavailability by two-fold over that of OM-NS.

  19. Preparation and evaluation of advanced catalysts for phosphoric acid fuel cells

    Science.gov (United States)

    Stonehart, P.; Baris, J.; Hockmuth, J.; Pagliaro, P.

    1984-01-01

    The platinum electrocatalysts were characterized for their crystallite sizes and the degree of dispersion on the carbon supports. One application of these electrocatalysts was for anodic oxidation of hydrogen in hot phosphoric acid fuel cells, coupled with the influence of low concentrations of carbon monoxide in the fuel gas stream. In a similar way, these platinum on carbon electrocatalysts were evaluated for oxygen reduction in hot phosphoric acid. Binary noble metal alloys were prepared for anodic oxidation of hydrogen and noble metal-refractory metal mixtures were prepared for oxygen reduction. An exemplar alloy of platinum and palladium (50/50 atom %) was discovered for anodic oxidation of hydrogen in the presence of carbon monoxide, and patent disclosures were submitted. For the cathode, platinum-vanadium alloys were prepared showing improved performance over pure platinum. Preliminary experiments on electrocatalyst utilization in electrode structures showed low utilization of the noble metal when the electrocatalyst loading exceeded one weight percent on the carbon.

  20. Evaluation of preparation methods for MS-based analysis of intestinal epithelial cell proteomes

    DEFF Research Database (Denmark)

    Hesselager, Marianne Overgaard; Codrea, Marius Cosmin; Bendixen, Emøke

    2015-01-01

    The gut epithelium formed between an organism and the environment plays an essential role in host–microbe interactions, yet remains one of the least characterized mammalian tissues. Especially the membrane proteins, which are critical to bacterial adhesion, are understudied, because these proteins...... are low in abundance, and large amounts of sample is needed for their preparation and for undertaking MS-based analysis. The aim of this study was to evaluate three different methods for isolation and preparation of pig intestinal epithelial cells for MS-based analysis of the proteome. Samples were...... of ease and speed of sample preparation, as well as protein recovery. In comparison, more gentle methods where intestinal epithelial cells are harvested by shaking are more time consuming, result in lower protein yield, and are prone to increased technical variation due to multiple steps involved....

  1. Section 608 Technician Certification

    Science.gov (United States)

    Certifications for technicians who maintain, service, repair, or dispose of equipment that could release ozone depleting refrigerants or, after January 1, 2018, substitute refrigerants into the atmosphere.

  2. Medical Certification System -

    Data.gov (United States)

    Department of Transportation — Provides automated risk-based decision making capability in support of medical certification and clearances processing associated fees and supporting surveillance of...

  3. RSES heat pump technician certification

    Energy Technology Data Exchange (ETDEWEB)

    Zeiner, J.

    1996-06-01

    In 1987 the National Heat Pump certification test was developed by the Refrigeration Service Engineers Society (RSES), and in 1994, the program was more specifically named Heat Pump Service Technician Certification. This report describes the benefits of certification.

  4. Guidelines for the verification and validation of expert system software and conventional software: Evaluation of knowledge base certification methods. Volume 4

    Energy Technology Data Exchange (ETDEWEB)

    Miller, L.A.; Hayes, J.E.; Mirsky, S.M. [Science Applications International Corp., McLean, VA (United States)

    1995-03-01

    This report presents the results of the Knowledge Base Certification activity of the expert systems verification and validation (V&V) guideline development project which is jointly funded by the US Nuclear Regulatory Commission and the Electric Power Research Institute. The ultimate objective is the formulation of guidelines for the V&V of expert systems for use in nuclear power applications. This activity is concerned with the development and testing of various methods for assuring the quality of knowledge bases. The testing procedure used was that of behavioral experiment, the first known such evaluation of any type of V&V activity. The value of such experimentation is its capability to provide empirical evidence for -- or against -- the effectiveness of plausible methods in helping people find problems in knowledge bases. The three-day experiment included 20 participants from three nuclear utilities, the Nuclear Regulatory Commission`s Technical training Center, the University of Maryland, EG&G Idaho, and SAIC. The study used two real nuclear expert systems: a boiling water reactor emergency operating procedures tracking system and a pressurized water reactor safety assessment systems. Ten participants were assigned to each of the expert systems. All participants were trained in and then used a sequence of four different V&V methods selected as being the best and most appropriate for study on the basis of prior evaluation activities. These methods either involved the analysis and tracing of requirements to elements in the knowledge base (requirements grouping and requirements tracing) or else involved direct inspection of the knowledge base for various kinds of errors. Half of the subjects within each system group used the best manual variant of the V&V methods (the control group), while the other half were supported by the results of applying real or simulated automated tools to the knowledge bases (the experimental group).

  5. Preparation and corrosion behavior evaluation of amalgam/titania nano composite

    OpenAIRE

    Neda Bahremandi Tolou; Mohammadhossein Fathi; Ahmad Monshi; Vajihesadat Mortazavi; Farzaneh Shirani

    2011-01-01

    Background: Many attempts have been performed and continued for improvement of dental amalgam properties during last decades. The aim of present research was fabrication and characterization of amalgam/titania nano composite and evaluation of its corrosion behavior. Materials and Methods: In this experimental research, nano particles of titania were added to initial amalgam alloy powder and then, dental amalgam was prepared. In order to investigate the effect of nano particle amounts on p...

  6. EVALUATION OF IN-VITRO ANTIOXIDANT ACTIVITY OF PANCHAGAVYA: A TRADITIONAL AYURVEDIC PREPARATION

    OpenAIRE

    Subrata De et al

    2012-01-01

    Panchagavya, a classical Ayurvedic preparation, was evaluated for its antioxidant potential by HPTLC-DPPH bioautography method as well as assays for Ferric reducing antioxidant power (FRAP), DPPH – free radical scavenging activity (DPPH) and Superoxide radical scavenging activity. In addition total phenolic content was also estimated which was in fairly good amount. HPTLC-DPPH bioautography study revealed the presence of several antioxidant compounds in Panchagavya. In all the assays performe...

  7. Two new reference materials based on tobacco leaves: certification for over a dozen of toxic and essential elements.

    Science.gov (United States)

    Samczyński, Zbigniew; Dybczyński, Rajmund S; Polkowska-Motrenko, Halina; Chajduk, Ewelina; Pyszynska, Marta; Danko, Bożena; Czerska, Elżbieta; Kulisa, Krzysztof; Doner, Katarzyna; Kalbarczyk, Paweł

    2012-01-01

    The preparation, certification, and characterization of two new biological certified reference materials for inorganic trace analysis have been presented. They are based on two different varieties of tobacco leaves, namely, Oriental Basma Tobacco Leaves (INCT-OBTL-5), grown in Greece, and Polish Virginia Tobacco Leaves (INCT-PVTL-6), grown in Poland. Certification of the materials was based on the statistical evaluation of results obtained in a worldwide interlaboratory comparison, in which 87 laboratories from 18 countries participated, providing 2568 laboratory averages on nearly 80 elements. It was possible to establish the certified values of concentration for many elements in the new materials, that is, 37 in INCT-OBTL-5 and 36 in INCT-PVTL-6, including several toxic ones like As, Cd, Hg, Pb, and so forth. The share and the role of instrumental analytical techniques used in the process of certification of the new CRMs are discussed.

  8. Preparation of electromechanically active silicone composites and some evaluations of their suitability for biomedical applications

    Energy Technology Data Exchange (ETDEWEB)

    Iacob, Mihail; Bele, Adrian [“Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41A, Iasi 700487 (Romania); Patras, Xenia [“Apollonia” University, 2 Muzicii Street, 700511 Iasi (Romania); Pasca, Sorin [“Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine Iaşi, Aleea Mihail Sadoveanu nr. 3, Iasi 700490 (Romania); Butnaru, Maria [“Gr. T. Popa” University of Medicine and Pharmacy, Faculty of Medical Bioengineering, 16 University Street, 700115 Iasi (Romania); Alexandru, Mihaela [“Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41A, Iasi 700487 (Romania); Ovezea, Dragos [National Institute for Research and Development in Electrical Engineering ICPE-CA, 313 Splaiul Unirii, Bucharest 030138 (Romania); Cazacu, Maria, E-mail: mcazacu@icmpp.ro [“Petru Poni” Institute of Macromolecular Chemistry, Aleea Gr. Ghica Voda 41A, Iasi 700487 (Romania)

    2014-10-01

    Some films based on electromechanically active polymer composites have been prepared. Polydimethylsiloxane-α,ω-diols (PDMSs) having different molecular masses (Mv = 60 700 and Mv = 44 200) were used as matrix in which two different active fillers were incorporated: titanium dioxide in situ generated from its titanium isopropoxide precursor and silica particles functionalized with polar aminopropyl groups on surface. A reference sample based on simple crosslinked PDMS was also prepared. The composites processed as films were investigated to evaluate their ability to act as efficient electromechanical actuators for potential biomedical application. Thus, the surface morphology of interest for electrodes compliance was analysed by atomic force microscopy. Mechanical and dielectric characteristics were evaluated by tensile tests and dielectric spectroscopy, respectively. Electromechanical actuation responses were measured by interferometry. The biocompatibility of the obtained materials has been verified through tests in vitro and, for valuable films, in vivo. The experimental, clinical and anatomopathological evaluation of the in vivo tested samples did not reveal significant pathological modifications. - Highlights: • Silicone composites differing by the filler and matrix characteristics were prepared. • Stress–strain curves were registered in normal and cyclic modes for composite films. • The dielectric permittivity, dielectric loss, and conductivity were determined. • Electromechanical response of the films was measured at an applied voltage. • Some biocompatibility tests, both in vitro and in vivo, were performed.

  9. Preparation and in vitro evaluation of a pluronic lecithin organogel containing ricinoleic acid for transdermal delivery.

    Science.gov (United States)

    Boddu, Sai Hs; Bonam, Sindhu Prabha; Wei, Yangjie; Alexander, Kenneth

    2014-01-01

    The present study deals with the preparation and in vitro evaluation of a Pluronic lecithin organogel gel containing ricinoleic acid for transdermal delivery. Blank Pluronic lecithin organogel gels were prepared using ricinoleic acid as the oil phase and characterized for pH, viscosity, gelation temperature, and microscopic structure. The optimized Pluronic lecithin organogel gel formulation was further evaluated using ketoprofen (10%) and dexamethasone (0.5%) as model drugs. The stability and in vitro permeability of ketoprofen and dexamethasone was evaluated and compared with the corresponding control formulation (Pluronic lecithin organogel gel made with isopropyl palmitate as the oil phase). The pH and viscosity of blank Pluronic lecithin organogel gel prepared with ricinoleic acid was comparable with the isopropyl palmitate Pluronic lecithin organogel gel. The thixotropic property of ricinoleic acid Pluronic lecithin organogel gel was found to be better than the control. Drug-loaded Pluronic lecithin organogel gels behaved in a similar manner and all formulations were found to be stable at 25 degrees C, 35 degrees C, and 40 degrees C for up to 35 days. The penetration profile of dexamethasone was similar from both the Pluronic lecithin organogel gels, while the permeability for ketoprofen from Pluronic lecithin organogel gel containing ricinoleic acid was found to be three times higher as compared to the control formulation.

  10. EAS Telecommunications Certification Bodies (TCB)

    Data.gov (United States)

    Federal Communications Commission — EAS (Equipment Authorization System). A Telecommunication Certification Body (TCB) is an accredited product certification body with the authority to issue Grants of...

  11. Zend PHP 5 certification study guide

    CERN Document Server

    Shafik, Davey

    2007-01-01

    Zend's new PHP 5 Certification Exam represent an excellent tool for professional PHP developers who want to distinguish themselves in their field. php|architect's Zend PHP 5 Certification Study Guide, edited and produced by the publishers of php|architect magazine, provides the most comprehensive and thorough preparation tool for developers who wish to take the exam. This book provides complete coverage of every topic that is part of the exam, including: PHP Basics, Functions, Arrays, Strings and Patterns, Web Programming, Object Oriented Programming, Database Programming, Object-oriented Design, XML and Web Services, Security, Streams and Network Programming, Differences Between PHP 4 and 5.

  12. Soy production and certification

    DEFF Research Database (Denmark)

    Tomei, Julia; Semino, Stella Maris; Paul, Helena

    2010-01-01

    With the rising emphasis on biofuels as a potential solution to climate change, this paper asks whether certification schemes, developed to promote sustainable feedstock production, are able to deliver genuine sustainability benefits. The Round Table on Responsible Soy (RTRS) is a certification s...

  13. Converse Barrier Certificate Theorems

    DEFF Research Database (Denmark)

    Wisniewski, Rafael; Sloth, Christoffer

    2016-01-01

    This paper shows that a barrier certificate exists for any safe dynamical system. Specifically, we prove converse barrier certificate theorems for a class of structurally stable dynamical systems. Other authors have developed a related result by assuming that the dynamical system has neither sing...

  14. Certification Programs for Citrus

    Science.gov (United States)

    Citrus certification programs designed to ensure that healthy plants of the highest genetic potential are being planted in the field are the basic building block of an integrated pest management program. Certification programs began for citrus began with the discovery that the diseases were graft t...

  15. QUALITY AND SHELF LIFE EVALUATION OF NUGGETS PREPARED FROM SPENT DUCK AND SPENT HEN MEAT

    Directory of Open Access Journals (Sweden)

    Rajesh Kumar

    2015-12-01

    Full Text Available A study was conducted to compare the quality of nuggets prepared from spent hen and duck meat. The cooked nuggets were analyzed for pH, thiobarbituric acid (TBA, tyrosine value (TV, moisture, fat, protein, total plate count (TPC and sensory evaluations. Nuggets prepared from spent hen meat showed significantly higher (p<0.05 moisture content however pH, fat and protein content were significantly higher (p<0.05 in duck nuggets. TBA values, TVs and (TPC were highest in duck nuggets but were within the acceptable level up to 7th day of refrigerated storage (4±1°C in both types of nuggets. Both nuggets maintain their sensory quality up to 7th day of refrigeration storage but spent hen nuggets were preferred by consumers compared to nuggets prepared from spent duck meat. Result of the study indicated that, despite the comparative differences among these nuggets, spent duck and hen meat could be used for preparation of nutritionally rich and acceptable nuggets.

  16. Preparation and evaluation of microcapsules using polymerized rosin as a novel wall forming material.

    Science.gov (United States)

    Fulzele, S V; Satturwar, P M; Kasliwal, R H; Dorle, A K

    2004-02-01

    Sustained release diclofenac sodium microcapsules were prepared using polymerized rosin as a novel wall-forming material by a solvent evaporation technique. A novel method developed in our laboratory with the potential for scale-up and production of polymerized rosin microcapsules is detailed. These microcapsules might have application for development of implant/depot systems, primarily due to a sustained/controlled release capability and potential biocompatibility of polymerized rosin. The effect of variables like solvent systems, stirring speed and temperature were previously optimized. The solution system of drug and polymerized rosin dissolved in iso-propyl alcohol and acetone is sprayed with the help of a 0.5 mm nozzle spray gun in liquid paraffin maintained at 60 degrees C in the stirring condition. Varying drug:polymer ratios, namely 1:1, 1:2, 2:1, 1:3 and 3:1, were employed for microcapsule preparation. The prepared microcapsules were evaluated for size, shape, drug content and in vitro drug release. The morphology of microcapsules was characterized by scanning electron microscopy. The microcapsules show sustained release curves at pH 7.4 phosphate buffer for up to 10 h. The data obtained from the dissolution profiles were compared in the light of different kinetics models and the regression coefficients were compared. The in vitro dissolution study confirmed the Higuchi-order release pattern. Particle size and release data analysis from five consecutive batches prepared in the laboratory indicated suitable reproducibility of the proposed solvent evaporation process.

  17. Evaluation of a filter-syringe set for preparation of packed cell aliquots for neonatal transfusion.

    Science.gov (United States)

    Chambers, L A

    1995-09-01

    A closed-system filter-syringe set designed for preparation of prefiltered aliquots of packed red cells for neonatal transfusion was evaluated. In three experiments, filter-syringe sets were sterile-connected, and aliquots were prepared at six intervals during 35-day storage of CPDA-1 packed cells. Hemoglobin (HGB), supernatant potassium (K+), and free hemoglobin (fHGB) in the aliquot and primary storage container were compared. There was no tubing weld failure, filter-occlusion, or bacterial contamination of the units. Hemoglobin remained stable over 35 days of storage, with comparable values in the primary collection container and syringe aliquot. Supernatant potassium in the units increased to an average of 89.5 mEq/L at 35 days, and levels in the aliquots closely matched those in the primary containers at each storage interval (maximum average 91.8 mEq/L). Free hemoglobin also progressively and comparably increased in both the primary containers and syringe aliquots. The gentle negative pressure and turbulence during use of the filter-syringe set apparently causes little hemolysis. The set simplifies preparation of aliquots for neonatal small volume and syringe-pump transfusion at costs comparable to alternative preparation methods. When sterile-connected, the filter-syringe set facilitates "assigned unit" inventory management for neonatal transfusion, which has been shown to be highly effective in limiting donor exposures.

  18. Evaluation of an intravenous preparation information system for improving the reconstitution and dilution process.

    Science.gov (United States)

    Jo, Yun Hee; Shin, Wan Gyoon; Lee, Ju-Yeun; Yang, Bo Ram; Yu, Yun Mi; Jung, Sun Hoi; Kim, Hyang Sook

    2016-10-01

    There are very few studies reporting the impact of providing intravenous (IV) preparation information on quality use of antimicrobials, particularly regarding their reconstitution and dilution. Therefore, to improve these processes in IV antimicrobial administration, an IV preparation information system (IPIS) was implemented in a hospital. We aimed to evaluate the effect of improving reconstitution and dilution by implementing an IPIS in the electronic medical record (EMR) system. Prescriptions and activity records of nurses for injectable antimicrobials that required reconstitution and dilution for IV preparation from January 2008 to December 2013 were retrieved from EMR, and assessed based on packaging label information for reconstituting and diluting solutions. We defined proper reconstitution and dilution as occurring when the reconstitution and dilution solutions prescribed were consistent with the nurses' acting records. The types of intervention in the IPIS were as follows: a pop-up alert for proper reconstitution and passive guidance for proper dilution. We calculated the monthly proper reconstitution rate (PRR) and proper dilution rate (PDR) and evaluated the changes in these rates and trends using interrupted time series analyses. Prior to the initiation of the reconstitution alert and dilution information, the PRR and PDR were 12.7 and 46.1%, respectively. The reconstitution alert of the IPIS rapidly increased the PRR by 41% (pinformation on dilution solutions was ineffective. Furthermore, solutions to ensure the continuous effectiveness of alert systems are warranted and should be actively sought. Copyright © 2016. Published by Elsevier Ireland Ltd.

  19. The effectiveness evaluation of indigenous microorganism (IMO preparations in the growth of onion (Allium cepa L.

    Directory of Open Access Journals (Sweden)

    Ramón Liriano González

    2015-02-01

    Full Text Available The experiment was done at the Credit and Services Cooperative (CCS “Juan de Mata Reyes”, in Pedro Betancourt municipality, Matanzas. The objective was to evaluate the effectiveness of indigenous microorganisms (IMO preparations on the growth of onion (Allium cepa L., variety Texas Early Grano. Five treatments were studied (control, a 2 mL.m2 treatment before transplantion, a 4 mL.m2 treatment before transplantation, and both 2 and 4 mL.m2 treatments 20 days after transplantion. The experiment was conducted in a randomized block design. The professional statistical pack STATISTICA, version 6.0 for WINDOWS was used for data processing. The results were statistically processed using simple analysis of variance and the Duncan´s multiple comparison Test for media with a level of significance of pd”0.05. The native microorganisms in the preparations were characterized. 60 days after transplantation, the parameters height of plant, number of leaves and diameter of the false stem were evaluated; and at harvest time, the total yield (t.ha-1 and the number of bulbs in diameter category were determined. The best results concerning the height of the plant (cm, diameter of false stem (mm and total yield (t.ha-1.were obtained with the preparation of 2 mL.m2 at the moment of transplantion, and 20 days after the application.

  20. Optimization and Evaluation Strategy of Esophageal Tissue Preparation Protocols for Metabolomics by LC-MS.

    Science.gov (United States)

    Wang, Huiqing; Xu, Jing; Chen, Yanhua; Zhang, Ruiping; He, Jiuming; Wang, Zhonghua; Zang, Qingce; Wei, Jinfeng; Song, Xiaowei; Abliz, Zeper

    2016-04-01

    Sample preparation is a critical step in tissue metabolomics. Therefore, a comprehensive and systematic strategy for the screening of tissue preparation protocols is highly desirable. In this study, we developed an Optimization and Evaluation Strategy based on LC-MS to screen for a high-extractive efficiency and reproducible esophageal tissue preparation protocol for different types of endogenous metabolites (amino acids, carnitines, cholines, etc.), with a special focus on low-level metabolites. In this strategy, we first selected a large number of target metabolites based on literature survey, previous work in our lab, and known metabolic pathways. For these target metabolites, we tested different solvent extraction methods (biphasic solvent extraction, two-step [TS], stepwise [SW], all-in one [AO]; single-phase solvent extraction, SP) and esophageal tissue disruption methods (homogenized wet tissue [HW], ground wet tissue [GW], and ground dry tissue [GD]). A protocol involving stepwise addition of solvents and a homogenized wet tissue protocol (SWHW) was superior to the others. Finally, we evaluated the stability of endogenous metabolites in esophageal tissues and the sensitivity, reproducibility, and recovery of the optimal protocol. The results proved that the SWHW protocol was robust and adequate for bioanalysis. This strategy will provide important guidance for the standardized and scientific investigation of tissue metabolomics.

  1. Evaluation of ultrasonic atomization as a new approach to prepare ionically cross-linked chitosan microparticles.

    Science.gov (United States)

    Albertini, Beatrice; Passerini, Nadia; Rodriguez, Lorenzo

    2005-07-01

    Ultrasonic atomization was evaluated as a new approach for the preparation of ionically cross-linked controlled-release chitosan microparticles loaded with theophylline as the model drug, using tripolyphosphate (TPP) as counter-ion. It was possible to nebulize both 2% and 3% (w/v) chitosan solutions as a function of their viscosity, usually not processed by employing the conventional nebulizer. The results of the chitosan molecular characterization using the SEC-MALS analysis revealed that ultrasonic atomization caused a certain depolymerization, probably due to the main chain scission of the 1,4-glycosidic bond; however, Fourier transform-infrared spectroscopy revealed the absence of other chemical modifications. The ultrasonic atomization allowed preparation of TPP cross-linked chitosan microparticles mostly ranging between 50 and 200 mum. As regards manufacturing parameters, the linking time and washing medium were found to affect the properties of the microparticles, while the stirring rate of the TPP solution did not show any influence. The evaluation of the formulation variables revealed that chitosan concentration strongly affected both the feasibility of the ultrasonic atomization and the drug release. All the microparticles showed an encapsulation efficiency of > 50 % and, after an initial burst effect, a controlled release of drug for 48 h. In conclusion, the ultrasonic atomization could be proposed as a robust and innovative single-step procedure with scale-up potential to successfully prepare ionically cross-linked chitosan microparticles.

  2. Evaluation of neon focused ion beam milling for TEM sample preparation.

    Science.gov (United States)

    Pekin, T C; Allen, F I; Minor, A M

    2016-10-01

    Gallium-based focused ion beams generated from liquid-metal sources are widely used in micromachining and sample preparation for transmission electron microscopy, with well-known drawbacks such as sample damage and contamination. In this work, an alternative (neon) focused ion beam generated by a gas field-ionization source is evaluated for the preparation of electron-transparent specimens. To do so, electron-transparent sections of Si and an Al alloy are prepared with both Ga and Ne ion beams for direct comparison. Diffraction-contrast imaging and energy dispersive x-ray spectroscopy are used to evaluate the relative damage induced by the two beams, and cross-sections of milled trenches are examined to compare the implantation depth with theoretical predictions from Monte Carlo simulations. Our results show that for the beam voltages and materials systems investigated, Ne ion beam milling does not significantly reduce the focused ion beam induced artefacts. However, the Ne ion beam does enable more precise milling and may be of interest in cases where Ga contamination cannot be tolerated.

  3. 49 CFR 583.13 - Supplier certification and certificates.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Supplier certification and certificates. 583.13... § 583.13 Supplier certification and certificates. Each supplier shall certify the information on each... supplier in any mode (e.g., paper, electronic) provided the mode contains all information in...

  4. Certification Report: Army Aviation Alternative Fuels Certification Program

    Science.gov (United States)

    2016-08-01

    SPECIAL REPORT RDMR-AE-16-02 CERTIFICATION REPORT: ARMY AVIATION ALTERNATIVE FUELS CERTIFICATION PROGRAM Dale Cox...NOT TO BE CONSTRUED AS AN OFFICIAL DEPARTMENT OF THE ARMY POSITION UNLESS SO DESIGNATED BY OTHER AUTHORIZED DOCUMENTS. TRADE NAMES USE...COVERED Final 4. TITLE AND SUBTITLE Certification Report: Army Aviation Alternative Fuels Certification Program 5. FUNDING NUMBERS 6

  5. Preparation and evaluation of tubular micelles of pluronic lecithin organogel for transdermal delivery of sumatriptan.

    Science.gov (United States)

    Agrawal, Varsha; Gupta, Vandana; Ramteke, Suman; Trivedi, Piyush

    2010-12-01

    The present work focuses on the preparation and evaluation of lecithin organogel system of thermoreversible polymer pluronic F127, which would enhance the stability and absorption of sumatriptan succinate across the skin. Formulations were developed with and without co-surfactant (pluronic F127). The prepared organogels were evaluated for its appearance, organoleptic characteristics, and feel upon application, homogeneity, occlusivenes, washability, pH, viscosity, spreadability, gel transition temperature of formulations. The formulations were also evaluated for drug content, in vitro drug diffusion properties and skin irritation testing. In vivo evaluation of formulations was carried out by hot plate and writhing test method, and finally the optimized formulation was subjected to stability studies. The developed formulations were easily washable, smooth in feel, and showed no clogging which indicate superior texture of system. Formulation, containing pluronic showed greater spreadability and higher drug diffusion rate as compared to pluronic free organogel. Drug content of organogel formulations was in the range of 94-97%. The pH of the formulations was 6.48 ± 0.5 and 6.98 ± 0.1, reflecting no risk of skin irritation. Pluronic not only enhances the stability of organogel by increasing the viscosity (from 6,541 ± 234.76 to 7,826 ± 155.65 poise) but also increases the release of drug from 67.39 ± 1.53% to 74.21 ± 1.7%. The sumatriptan exhibits higher and long lasting antinociceptive effect as indicated by the persistent increase in reaction time in hot plate and inhibited abdominal contraction in acetic acid-induced writhing test (p < 0.05). The prepared optimized formulation was found to be stable without any significant changes at room temperature.

  6. National Certification in Plastics (NCP). NCP Certified Operator. Candidate Handbook. Second Edition.

    Science.gov (United States)

    Society of the Plastics Industry, Inc., Washington, DC.

    This handbook provides information for persons who are preparing to take a test for certification in the plastics industry. The nine sections of the handbook provide information on the following: (1) the mission of the Society of the Plastics Industry and the value of the National Certification in Plastics; (2) tips on preparing for the exam; (3)…

  7. Evaluation of the xerovac process for the preparation of heat tolerant contagious bovine pleuropneumonia (CBPP) vaccine.

    Science.gov (United States)

    Litamoi, J K; Ayelet, G; Rweyemamu, M M

    2005-04-01

    The study was conducted with the aim of evaluating the xerovac process as a method for preparing contagious bovine pleuropneumonia (CBPP) vaccine with increased heat resistance. The thermo-protective effects of various concentrations of trehalose in mycoplasma growth medium, various concentrations of trehalose in the dehydration stabilizer and the importance of some divalent cations were assessed. The results obtained indicate that a rapid dehydration of CBPP vaccine following the xerovac method and in an excipient composed of a high concentration of trehalose, renders the product more heat tolerant than a similar vaccine prepared using a regular or an extended freeze drying regime. It was also demonstrated that the addition of chitosan as a mycoplasma precipitating agent conferred additional heat resistance to the vaccine. It is suggested that the application of the xerovac process in the dehydration of CBPP vaccine offers the advantages of a faster, cheaper and easier process over the conventional dehydration methods like freeze drying.

  8. Evaluation of senior Brazilian dental students about mouth preparation and removable partial denture design.

    Science.gov (United States)

    Neto, Arcelino Farias; Duarte, Antônio Ricardo Calazans; Shiratori, Fábio Kenji; de Alencar e Silva Leite, Pedro Henrique; Rizzatti-Barbosa, Célia Marisa; Bonachela, Wellington Cardoso

    2010-11-01

    This study aimed to evaluate the knowledge of senior dental students about mouth preparation and removable partial denture (RPD) design. Two hundred sixty-six senior students from eleven dental schools in the State of São Paulo, Brazil, comprised the sample. The subjects examined two partially edentulous casts mounted on a semiadjustable articulator, answered a questionnaire regarding the treatment plan, and drew the RPD design. The casts consisted of Kennedy Class III, modification 1 maxillary arch and Class II mandibular arch. Ninety percent of the students believed that mouth preparation should be performed although no one was able to name all necessary procedures. For the maxillary arch, 12 percent of the denture designs were completely appropriate, 51 percent were partially appropriate, and 37 percent were inappropriate. For the mandibular arch, the results were 3 percent, 40 percent, and 57 percent, respectively.

  9. Preparation and antimicrobial evaluation of polyion complex (PIC) nanoparticles loaded with polymyxin B.

    Science.gov (United States)

    Insua, Ignacio; Majok, Sieta; Peacock, Anna F A; Krachler, Anne Marie; Fernandez-Trillo, Francisco

    2017-02-01

    Here, we describe novel polyion complex (PIC) particles for the delivery of Polymyxin B (Pol-B), an antimicrobial peptide currently used in the clinic as a last resort antibiotic against multidrug-resistant gram-negative bacteria. A range of conditions for the controlled assembly of Pol-B with poly(styrene sulphonate) (PSS) has been identified which let us prepare stable colloidal PIC particles. This way, PIC particles containing different Pol-B:PSS ratios have been prepared and their stability under simulated physiological conditions (i.e. pH, osmotic pressure and temperature) characterised. Furthermore, preliminary evaluation of the antimicrobial activity of these Pol-B containing PIC particles has been performed, by monitoring their effect on the growth of Pseudomonas aeruginosa, an opportunistic gram-negative bacterium.

  10. Prospective evaluation of 1-day polyethylene glycol-3350 bowel preparation regimen in children.

    Science.gov (United States)

    Abbas, Mazen I; Nylund, Cade M; Bruch, Carol J; Nazareno, Luzviminda G; Rogers, Philip L

    2013-02-01

    The aim of the present study was to evaluate efficacy, safety, and tolerability of a pediatric colonoscopy bowel preparation regimen composed of polyethylene glycol-3350 (PEG-3350) and a sports drink completed in a few hours. A prospective, open-label trial of a colonoscopy bowel preparation in children ages 8 to 18 years that included 238 g of PEG-3350 mixed with 1.9 L of Gatorade completed in a few hours. Efficacy was determined using the Boston Bowel Preparation Scale. Basic metabolic profiles and questionnaires were obtained that assessed for safety, adverse effects, tolerability, and patient acceptability. Forty-six patients completed the study. Patients were predominately boys (56.5%) with a mean age of 14.50 years (SD ± 2.9 years). Forty-three (93.5%) were able to complete the regimen. All of the colonoscopies were completed to the cecum and 84% had terminal ileum visualization. Seventy-seven percent were found to be effective preparations. Nausea/vomiting were the most common reported adverse effect (60%) followed by abdominal pain/cramping (44%) and fatigue/weakness (40%). Overall, the regimen was acceptable with 1 exception being the large volume to drink. There were no clinically significant changes in basic metabolic profiles, although there was a statistically significant decrease in the mean potassium (0.16 mEq/L; P = 0.016), blood urea nitrogen (2.68 mg/dL; P Gatorade administered in a few hours is an effective, safe, and moderately tolerable bowel preparation regimen for colonoscopy in children.

  11. PREPARATION AND EVALUATION OF VITAMIN E ADJUVANTED OIL EMULSIFIED INFECTIOUS BRONCHITIS EXPERIMENTAL VACCINE

    Directory of Open Access Journals (Sweden)

    S. ALI, M. ARSHAD, M. SIDDIQUE AND M. ASHRAF

    2007-10-01

    Full Text Available The present study was conducted to prepare oil emulsified (OE infectious bronchitis (IB experimental vaccines. The vaccines were prepared using the vaccinal strain H-120 Infectious Bonchitis virus (IBV. The virus was cultivated in 9-day old embryonated eggs via allantoic cavity route. Allantoic-amniotic fluid (AAF was collected and inactivated with formalin @ 0.12%. Water in oil emulsion was prepared by adding one part of AAF to four parts of mineral oil containing water phase (Tween 80 and oil phase (Span 80 surfactants. Hydrophile lypohile balance (HLB of the emulsion was maintained at 7.0. Two oil emulsified experimental vaccines were prepared. Vaccine-I was prepared without vitamin E and Vaccine-II with vitamin E (300 mg/ml. A total of 120 day-old broiler breeder chickens were divided into 4 groups, A, B, C, and D, each having 30 birds. At the age of 21 days, experimental Vaccine-I, experimental vaccine-II and commercial IB killed (H-120 vaccine were inoculated @ 0.5 ml in the birds of groups A, B and C, respectively. Group D was maintained as nonvaccinated control. Efficacy of the vaccines was evaluated on the basis of humoral immune response (haemagglutination inhibition antibody titres against IB in the four groups. The seven weeks cumulative mean antibody titres (CMT of each group were calculated. The highest CMT was observed in group B (130, followed by group C (69, group A (58 and group D (17. Statistical analysis showed that haemagglutination inhibition (HI antibody titres in group B (vaccine- II were significantly higher than those of groups A, B and C (P< 0.05.

  12. Preparation, characterization, and insecticidal activity evaluation of three different formulations of Beauveria bassiana against Musca domestica.

    Science.gov (United States)

    Mishra, Sapna; Kumar, Peeyush; Malik, Anushree

    2013-10-01

    Three formulations; bait, encapsulation, and emulsion of Beauveria bassiana were prepared and evaluated for their insecticidal activity in simulated field settings. Tea waste-based bait formulation of B. bassiana showed 100% mortality (within 72 h) in lab assay against adult houseflies. In field assay using traps, 65% relative entrapment and 100 % mortality (within 60 h) of entrapped flies was observed. Although the bait formulation was low cost and easy to prepare and transport, its storage ability was limited. Hence, more advanced formulations in form of encapsulation and emulsion was attempted. Encapsulated B. bassiana conidia (using skimmed milk powder, polyvinyl pyrrolidone K-90 and glucose as additives) showed 100% conidial germination and retained 78% conidial viability, even after storage for 12 months at 30 °C. Encapsulated product showed 54.8% (freshly prepared) and 30.6 % (after 12-months storage) mortality of housefly larvae in a simulated field condition. Emulsion formulation was prepared by using Tween 20 as surfactant with seven vegetable oils: soybean, rapeseed, sunflower, olive, castor, til, and linseed. Emulsion with linseed oil showing maximum conidial germination (94%) was evaluated for shelf life and pathogenecity against housefly larvae. Shelf life analysis of emulsion revealed 28% conidial germination and 19.9% housefly larval mortality after 12 months of storage as opposed to 94% conidial germination and 51.7% of larval mortality with fresh product. Significant increase in shelf × targeted application of formulation is expected to increase its mass applicability for housefly control. Also, the variability among products presents diverse opportunities for commercialization.

  13. Producer-level benefits of sustainability certification.

    Science.gov (United States)

    Blackman, Allen; Rivera, Jorge

    2011-12-01

    Initiatives certifying that producers of goods and services adhere to defined environmental and social-welfare production standards are increasingly popular. According to proponents, these initiatives create financial incentives for producers to improve their environmental, social, and economic performance. We reviewed the evidence on whether these initiatives have such benefits. We identified peer-reviewed, ex post, producer-level studies in economic sectors in which certification is particularly prevalent (bananas, coffee, fish products, forest products, and tourism operations), classified these studies on the basis of whether their design and methods likely generated credible results, summarized findings from the studies with credible results, and considered how these findings might guide future research. We found 46 relevant studies, most of which focused on coffee and forest products and examined fair-trade and Forest Stewardship Council certification. The methods used in 11 studies likely generated credible results. Of these 11 studies, nine examined the economic effects and two the environmental effects of certification. The results of four of the 11 studies, all of which examined economic effects, showed that certification has producer-level benefits. Hence, the evidence to support the hypothesis that certification benefits the environment or producers is limited. More evidence could be generated by incorporating rigorous, independent evaluation into the design and implementation of projects promoting certification.

  14. Microleakage Evaluation Around Retrograde Filling Materials Prepared Using Conventional and Ultrasonic Techniques

    Science.gov (United States)

    Bolla, Nagesh; Thumu, Jayaprakash; Vemuri, Sayesh; Chukka, Sunil

    2015-01-01

    Introduction: The importance of the retrograde cavity preparation and the material used to restore is of utmost importance to achieve successful surgical endodontics. Aim: The aim of the present study is to evaluate the apical micro-leakage of root end cavities filled with Mineral trioxide aggregate, Biodentine and light cure GIC using two different cavity preparation techniques that is conventional bur preparation and ultrasonic tip preparation. Materials and Methods: Eighty extracted single rooted human teeth (except mandibular incisors) with one canal, fully developed apices and without any major carious lesion are collected for the study. The teeth were sectioned at CEJ to standardize the length. Roots are instrumented upto master apical file 40 K size and obturated with gutta percha and AH plus sealer in lateral condensation technique. The teeth were then resected apically at 90° angle axis to the long axis of the root removing 3 mm of the apex. The teeth were divided in to four groups of 20 each- • Group I- samples restored with MTA. • Group II- samples restored with Biodentine. • Group III- (Positive control group)- samples restored with Light activated GIC. • Group IV - (negative control group)- no filling material. Each group is divided into two subgroups (a, b) of ten teeth each 1. Retropreparation done with ultrasonic retrotip. 2. Retropreparation done with conventional bur. The teeth were then immersed in 0.5% Rhodamine B dye for 48 h. The teeth were split longitudinally and the interface between the restored material and the canal wall is observed under Confocal laser scanning microscope. Depth of dye penetration was examined under stereomicroscope. Results: The statistical analysis was performed by One way ANOVA, t test. Pair wise comparision was done by Newman – Keuls multiple post hoc test. The mean values of Dye penetration for Group Ia (321.23), Group Ib (490.11), Group IIa (1065.14), Group IIb (1170.96), Group IIIa (1888.90), Group

  15. Evaluating Curricular Influence on Preparation for Practice, Career Outcomes, and Job Satisfaction: Results from an Alumni Survey of a 40-Year Rehabilitation and Mental Health Counseling Program

    Science.gov (United States)

    Smith, Tammy Jorgensen; Reid, Joan A.; Henry, Ryan G.; Dixon, Charlotte G.; Wright, Tennyson J.

    2013-01-01

    Alumni of a Council on Rehabilitation Education (CORE)-accredited graduate rehabilitation counselor education (RCE) program were surveyed to evaluate career outcomes, job satisfaction, licensure and certification rates, client populations served, and RCE program satisfaction and effectiveness. Results indicate a high level of satisfaction with the…

  16. Evaluating Curricular Influence on Preparation for Practice, Career Outcomes, and Job Satisfaction: Results from an Alumni Survey of a 40-Year Rehabilitation and Mental Health Counseling Program

    Science.gov (United States)

    Smith, Tammy Jorgensen; Reid, Joan A.; Henry, Ryan G.; Dixon, Charlotte G.; Wright, Tennyson J.

    2013-01-01

    Alumni of a Council on Rehabilitation Education (CORE)-accredited graduate rehabilitation counselor education (RCE) program were surveyed to evaluate career outcomes, job satisfaction, licensure and certification rates, client populations served, and RCE program satisfaction and effectiveness. Results indicate a high level of satisfaction with the…

  17. Evaluation of Root Canal Preparation Using Rotary System and Hand Instruments Assessed by Micro-Computed Tomography

    OpenAIRE

    Stavileci, Miranda; Hoxha, Veton; Görduysus, Ömer; Tatar, Ilkan; Laperre, Kjell; Hostens, Jeroen; Küçükkaya, Selen; Muhaxheri, Edmond

    2015-01-01

    Background Complete mechanical preparation of the root canal system is rarely achieved. Therefore, the purpose of this study was to evaluate and compare the root canal shaping efficacy of ProTaper rotary files and standard stainless steel K-files using micro-computed tomography. Material/Methods Sixty extracted upper second premolars were selected and divided into 2 groups of 30 teeth each. Before preparation, all samples were scanned by micro-computed tomography. Thirty teeth were prepared w...

  18. Converse Barrier Certificate Theorem

    DEFF Research Database (Denmark)

    Wisniewski, Rafael; Sloth, Christoffer

    2013-01-01

    This paper presents a converse barrier certificate theorem for a generic dynamical system.We show that a barrier certificate exists for any safe dynamical system defined on a compact manifold. Other authors have developed a related result, by assuming that the dynamical system has no singular...... points in the considered subset of the state space. In this paper, we redefine the standard notion of safety to comply with generic dynamical systems with multiple singularities. Afterwards, we prove the converse barrier certificate theorem and illustrate the differences between ours and previous work...

  19. Employment certificates on HRT

    CERN Multimedia

    HR Department

    2008-01-01

    As part of the ongoing drive to simplify and streamline administrative procedures and processes, the IT and HR Departments have made employment certificates available on a self-service basis on the HRT application, in the main menu under "My self services". All members of the personnel can thus obtain a certificate of employment or association, in French or in English, for the present or past contractual period. The HR Department’s Records Office remains responsible for issuing any special certificates that might be required. IT-AIS (Administrative Information Services) HR-SPS (Services, Procedures & Social) Records Office – Tel. 73700

  20. CFPC's Certification Examination

    Science.gov (United States)

    Handfield-Jones, Richard; Hollingworth, Gary R.

    1990-01-01

    The certification examination of the College of Family Physicians of Canada is designed to assess the extent to which the College's educational objectives have been achieved. Since the first examination in 1969, more than 7000 physicians have received their certification. The authors describe the basic elements of this test and the process through which the Committee on Examinations designs and sets the examination. The authors comment on the role of the certification process in the education of family physicians in Canada. Imagesp2073-a PMID:21233952

  1. Practitioner Expectations and Experiences with the Certificate IV in Training and Assessment (TAA40104). A National Vocational Education and Training Research and Evaluation Program Report

    Science.gov (United States)

    Clayton, Berwyn; Meyers, Dave; Bateman, Andrea; Bluer, Robert

    2010-01-01

    The Certificate IV in Training and Assessment (TAA40104) is seen as the standard entry-level teaching qualification in the vocational education and training (VET) sector. The qualification is widely accepted and well supported as an essential requirement for VET practitioners. However, it has been criticised in relation to its ability to provide…

  2. Preparation and properties evaluation of biolubricants derived from canola oil and canola biodiesel.

    Science.gov (United States)

    Sharma, Rajesh V; Somidi, Asish K R; Dalai, Ajay K

    2015-04-01

    This study demonstrates the evaluation and comparison of the lubricity properties of the biolubricants prepared from the feed stocks such as canola oil and canola biodiesel. Biolubricant from canola biodiesel has a low cloud and pour point properties, better friction and antiwear properties, low phase transition temperature, is less viscous, and has the potential to substitute petroleum-based automotive lubricants. Biolubricant from canola oil has high thermal stability and is more viscous and more effective at higher temperature conditions. This study elucidates that both the biolubricants are attractive, renewable, and ecofriendly substitutes for the petroleum-based lubricants.

  3. Preparation and Evaluation of Immobilized SE-30 Coated Stationary Phases for CEC

    Institute of Scientific and Technical Information of China (English)

    NingBaoming; ZhengJie; ZhangGuodong; XuBingjiu

    2001-01-01

    A new type of stationary phase for capillary electrochromatography (CEC), immobilized SE-30 coated silica gel, was developed and the columns packed with this new phase were prepared and evaluated. It was found that this phase could be used to make frits for the micro-columns as well as to pack the bulk of the column. By sticking together the particles in the whole of the column bed, this new packing resulted in columns with stable performance, even under rigorous conditions: more than 380 consecutive separations were effected with these columns with the mobile phase pH of 11.7.

  4. PREPARATION AND EVALUATION OF SUSTAINED RELEASE MICROSPHERE OF NORFLOXACIN USING SODIUM ALGINATE

    Directory of Open Access Journals (Sweden)

    Raja Chakraverty

    2012-01-01

    Full Text Available Oral controlled drug delivery systems represent the most popular form of sustained drug delivery systems for the obvious advantages of oral route of drug administration. Such systems release the drug with constant or variable release rates. The oral controlled release systems shows a typical pattern of drug release in which the drug concentration is maintained in the therapeutic window for a prolonged period of time (sustained release, thereby ensuring sustained therapeutic action. They are used as single dosage form. Present work involves preparation and evaluation of sustained release of microspheres of Norfloxacin employing sodium alginate as natural polymer. The technique employed for microencapsulation of the drug is ionotropic gelation.

  5. EVALUATION OF IN-VITRO ANTIOXIDANT ACTIVITY OF PANCHAGAVYA: A TRADITIONAL AYURVEDIC PREPARATION

    Directory of Open Access Journals (Sweden)

    Subrata De et al

    2012-08-01

    Full Text Available Panchagavya, a classical Ayurvedic preparation, was evaluated for its antioxidant potential by HPTLC-DPPH bioautography method as well as assays for Ferric reducing antioxidant power (FRAP, DPPH – free radical scavenging activity (DPPH and Superoxide radical scavenging activity. In addition total phenolic content was also estimated which was in fairly good amount. HPTLC-DPPH bioautography study revealed the presence of several antioxidant compounds in Panchagavya. In all the assays performed, it showed considerable antioxidant activity. On comparison of the data of three different batches of the samples studied, it showed 98.3 - 99.8% correlation between total phenolic content, FRAP and DPPH assays.

  6. Community-wise evaluation of rice beer prepared by some ethnic tribes of Tripura

    Directory of Open Access Journals (Sweden)

    Sushanta Ghosh

    2016-12-01

    Full Text Available Tripura is inhabited by many indigenous communities having unique ethno-socio-cultural lifestyle with age-old rice beer brewing techniques using wild herbs and local rice varieties popularly known as chuwak or zu. The present study is focused on comparative evaluation of brewing methods and nutritional aspects of rice beer among Debbarma, Jamatia, Koloi, and Molsom tribes of Tripura. Sample ingredients and plant species are properly identified before reporting. Rice beer is also prepared in laboratory conditions for comparative studies of qualitative and quantitative aspects. Thirteen different plant species are used by these four tribes for preparation of starter cultures using soaked rice flour. Markhamia stipulate (Wall. Seem. is common to all communities for starter cake preparation. Litsea monopetala (Roxb. Pers. is used by all three communities except Jamatia. The use of Ananus comosus Mill. is common among Debbarma and Jamatia tribes, whereas that of Artocarpus heterophyllus Lam. is common among Molsom and Koloi tribes. However, Aporusa diocia (Roxb. Muell., Combretum indicum (L. DeFilipps., and Citrus sinensis (L. Osbeck. are used only by Debbarma tribe for unique tangy flavor. The physicochemical properties of rice beer varied within tribes for its moisture content, carbohydrate content, reducing sugar, and alcohol percentage. The concentration of alcohol increases with aging and prolonged fermentation. The plants reported here are also reported for having nutritional and medicinal benefits for the metabolic stability in humans, which make the process more prospective for commercialization if a standard for maintaining a quality and associated risk can be determined.

  7. Animated educational video to prepare children for MRI without sedation: evaluation of the appeal and value

    Energy Technology Data Exchange (ETDEWEB)

    Szeszak, Szofia [University of Nottingham, Radiological Sciences, Division of Clinical Neuroscience, Queen' s Medical Centre, Nottingham (United Kingdom); Man, Rachel; Love, Andrew [Nottingham Trent University, School of Art and Design, Nottingham (United Kingdom); Langmack, Gill; Wharrad, Heather [University of Nottingham, Health E-learning and Media group, School of Health Sciences, Queen' s Medical Centre, Nottingham (United Kingdom); Dineen, Robert A. [University of Nottingham, Radiological Sciences, Division of Clinical Neuroscience, Queen' s Medical Centre, Nottingham (United Kingdom); University of Nottingham, Sir Peter Mansfield Imaging Centre, Queen' s Medical Centre, Nottingham (United Kingdom)

    2016-11-15

    MRI scans can be distressing for children and often require sedation. Educating children about what to expect reduces anxiety and increases likelihood of successful non-sedated MRI scans. Multimedia tools are a popular means of education. Animated video could provide a free, accessible method of preparing children for MRI scans. To evaluate a new animation video for preparing children for MRI, specifically for decreasing in-scanner motion and examination failure. We recruited 24 healthy children ages 5-11 years. Participants underwent pre- and post-viewing questionnaires and structured interviews. We then compared median Likert scale score changes between pre- and post-animation questions and analyzed the interview framework. Participants were filmed viewing the animation to calculate time spent looking at the screen to assess how well the video retained children's attention. There were significant improvements in median scores regarding what to expect, checking for metal and keeping still. There were no significant changes in other knowledge-based topics. There were significant improvements in median scores for anxiety-based topics. On average, children watched the screen for 98.9% of the 174-s animation. The animation improved knowledge, reduced anxiety, retained attention and was enjoyed by participants. It can be accessed freely via the Internet to help prepare children ages 5-11 for having an MRI scan. (orig.)

  8. Bismuth Modified Porous Silica Preparation, Characterization and Photocatalytic Activity Evaluation for Degradation of Isoproturon

    Institute of Scientific and Technical Information of China (English)

    Anil Kumar Reddy Police; Srinivas Basavaraju; Durga Kumari Valluri; Subrahmanyam Machiraju

    2013-01-01

    Porous silica prepared by using an acrylic emulsion has been impregnated with bismuth ion resulting in Bi2Sio5 species containing surface.The as-prepared materials have been characterized by X-ray diffraction spectroscopy (XRD),X-ray photoelectron spectroscopy (XPS),UV-Vis diffuse reflectance spectroscopy (UV-Vis DRS),scanning electron microscopy (SEM),energy dispersive analysis of X-ray (EDAX),transmission electron microscopy (TEM),Fourier transform infrared spectroscopy (FTIR) and N2 adsorption/desorption techniques.EDAX analysis confirms the penetration of bismuth ions into the framework of silica to form Bi2Sio5,which is substantiated by XRD.The UV-Vis DRS shows that the catalysts are optically active and XPS confirms the inclusion of bismuth into the framework of silica.FTIR spectra illustrate the formation of Bi-O-Si linkages in the porous silica framework.SEM and TEM show the spherical morphology,whereas N2 adsorption/desorption study confirms the porosity of the prepared materials.The photocatalytic activity of the material is evaluated for the degradation of isoproturon herbicide and it is found that the material is active as compared to the commercial P-25 Degussa Tio2.

  9. PREPARATION AND EVALUATION OF MUCOADHESIVE MICROCAPSULES OF IBUPROFEN FOR CONTROLLED RELEASE

    Directory of Open Access Journals (Sweden)

    Bantu Appa Rao

    2011-05-01

    Full Text Available The aim of this study was to prepare and evaluate microcapsules containing ibuprofen employing sodium alginate in combination with mucoadhesive polymers namely methylcellulose, Hydroxypropylmethylcellulose and sodiumcaboxymethylcellulose. The microcapsules were prepared by orifice-ionic gelation method. The microcapsules prepared are spherical, discrete, free flowing and were of multinucleate and monolithic type. Microencapsulation efficiency was in the range of 77.81-91.41 %. The in vitro drug release of the microcapsules carried out in phosphate buffer pH7.2 and drug release from the microcapsules was slow over 12 h and depends on core:coat ratio, wall thickness and size of the microcapsules. The drug release from all the microcapsules followed non-fickian diffusion. Microcapsules of alginate- methylcellulose gave relatively fast release when compared to others. The order of release rate observed with various microcapsules was sodiumcaboxymethylcellulose < Hydroxypropylmethylcellulose < methylcellulose. Results of our present study suggest that ibuprofen microcapsules can be successfully designed to develop controlled drug delivery, which can improve compliance by reducing dosing frequency.

  10. Preparation of albumin based nanoparticles for delivery of fisetin and evaluation of its cytotoxic activity.

    Science.gov (United States)

    Ghosh, Pooja; Singha Roy, Atanu; Chaudhury, Susmitnarayan; Jana, Saikat Kumar; Chaudhury, Koel; Dasgupta, Swagata

    2016-05-01

    Fisetin is a well known flavonoid that shows several properties such as antioxidant, antiviral and anticancer activities. Its use in the pharmaceutical field is limited due to its poor aqueous solubility which results in poor bioavailability and poor permeability. The aim of our present study is to prepare fisetin loaded human serum albumin nanoparticles to improve its bioavailability. The nanoparticles were prepared by a desolvation method and characterized by spectroscopic and microscopic techniques. The particles were smooth and spherical in nature with an average size of 220 ± 8 nm. The encapsulation efficiency was found to be 84%. The in vitro release profile showed a biphasic pattern and the release rate increases with increase in ionic strength of solution. We have also confirmed the antioxidant activity of the prepared nanoparticles by a DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Further its anticancer activity was evaluated using MCF-7 breast cancer cell lines. Our findings suggest that fisetin loaded HSA nanoparticles could be used to transfer fisetin to target areas under specific conditions and thus may find use as a delivery vehicle for the flavonoid.

  11. PREPARATION AND IN VITRO AND IN VIVO EVALUATION OF BUCCOADHESIVE BILAYERED TABLETS OF DILTIAZEM HYDROCHLORIDE

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    D’souza A

    2012-08-01

    Full Text Available In this study buccoadhesive bilayered tablets of Diltiazem HCl (DTZ were prepared in order to improve the bioavailability by the avoidance of hepatic first-pass metabolism, and to prevent frequent administration. Bilayered Tablets containing fixed amount of Diltiazem HCl (DTZ were prepared by direct compression method using polymers like hydroxyl propyl methyl cellulose (HPMC K4M, HPMC K15M, HPMC K100M in combination with backing layer of ethyl cellulose and evaluated for physicochemical properties, swelling, bioadhesive strength, in vitro permeation studies, in vitro drug release and possible interaction between ingredients. The physicochemical properties, swelling index, surface pH, bioadhesive strength, in vitro drug release and in vitro permeation studies were found to be dependent on the grade and proportion of buccoadhesive material used. The dissolution of Diltiazem HCl from all the prepared tablets into phosphate buffer (pH 6.8 was controlled for 6 hrs and followed non-Fickian release mechanism. Lower release rates were observed for formulations containing higher concentration of higher viscosity grade of HPMC. FTIR and DSC studies revealed the absence of significant interaction between DTZ and the selected bioadhesive materials. In vivo studies of selected formulation in rabbits demonstrated significant enhancement in bioavailability of DTZ relative to orally administered drug.

  12. Cationic Liposomes Modified with Polyallylamine as a Gene Carrier: Preparation, Characterization and Transfection Efficiency Evaluation

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    Reza Kazemi Oskuee

    2016-12-01

    Full Text Available Purpose: Cationic polymers and cationic liposomes have shown to be effective non-viral gene delivery vectors. In this study, we tried to improve the transfection efficiency by employing the advantages of both. Methods: For this purpose, modified polyallylamines (PAAs were synthesized. These modifications were done through the reaction of PAA (15 KDa with acrylate and 6-bromoalkanoic acid derivatives. Liposomes comprising of these cationic polymers and cationic lipid were prepared and extruded through polycarbonate filters to obtain desired size. Liposome-DNA nanocomplexes were prepared in three carrier to plasmid (C/P ratios. Size, zeta potential and DNA condensation ability of each complex were characterized separately and finally transfection efficiency and cytotoxicity of prepared vectors were evaluated in Neuro2A cell line. Results: The results showed that mean particle size of all these nanocomplexes was lower than 266 nm with surface charge of 22.0 to 33.9 mV. Almost the same condensation pattern was observed in all vectors and complete condensation was occurred at C/P ratio of 1.5. The lipoplexes containing modified PAA 15 kDa with 10% hexyl acrylate showed the highest transfection efficacy and lowest cytotoxicity in C/P ratio of 0.5. Conclusion: In some cases nanocomplexes consisting of cationic liposome and modified PAA showed better transfection activity and lower cytotoxicity compared to PAA.

  13. Evaluation of scale-up from analytical to preparative supercritical fluid chromatography.

    Science.gov (United States)

    Enmark, Martin; Åsberg, Dennis; Leek, Hanna; Öhlén, Kristina; Klarqvist, Magnus; Samuelsson, Jörgen; Fornstedt, Torgny

    2015-12-18

    An approach for reliable transfer from analytical to preparative scale supercritical fluid chromatography was evaluated. Here, we accounted for the conditions inside the columns as well as to the fact that most analytical instruments are volume-controlled while most preparative scale units are mass-controlled. The latter is a particular problem when performing pilot scale experiments and optimizations prior to scaling up to production scale. This was solved by measuring the mass flow, the pressure and the temperature on the analytical unit using external sensors. Thereafter, it was revealed with a design of experiments approach that the methanol fraction and the pressure are the two most important parameters to control for preserved retention throughout the scale-up; for preserved selectivity the temperature was most important in this particular system. Using this approach, the resulting chromatograms from the preparative unit agreed well with those from the analytical unit while keeping the same column length and particles size. A brief investigation on how the solute elution volume varies with the volumetric flow rate revealed a complex dependency on pressure, density and apparent methanol content. Since the methanol content is a parameter of great importance to control during the scale up, we must be careful when changing operational and column design conditions which generates deviations in pressure, density and methanol content between different columns. Copyright © 2015 Elsevier B.V. All rights reserved.

  14. Biological Evaluation of Polyherbal Ayurvedic Cardiotonic Preparation “Mahamrutyunjaya rasa”

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    Pallavi D. Rai

    2011-01-01

    Full Text Available Mahamrutyunjaya rasa (MHR, an Ayurvedic formulation, used as cardiotonic, contains potentially toxic compounds like aconitine, which are detoxified during preparation using traditional methods. Comparative toxicological evaluation of laboratory prepared formulation (F1 and two marketed formulations (F2 and F3 were performed based on their effects on viability of H9c2 cells and after single oral dose administration in mice. Cardioprotective effect of formulations at 25 and 50 mg/kg doses were studied in isoproterenol- (ISO- induced myocardial infarcted rats. F1 and F2 did not affect the cell viability, while F3 decreased the cell viability in concentration and time-dependent manner. Rats administered with ISO showed significant increase in the serum levels of glutamate oxaloacetate transaminase, alkaline phosphotase, creatinine kinase isoenzymes, lactate dehydrogenase, and uric acid, while F1 and F2 treatment showed significant reduction in the same. F3 showed further increase in the serum levels of enzymes and uric acid in ISO-challenged rats. High pressure liquid chromatographic analysis of formulations showed higher concentration of aconitine in F3. Study shows that F1 and F2 possess cardioprotective property with higher safety, while formulation F3 cannot be used as cardioprotective due to its cytotoxic effects. Thus, proper quality assessment methods are required during preparation of traditional formulations.

  15. Evaluation of three Campylobacter pylori antigen preparations for screening sera from patients undergoing endoscopy.

    Science.gov (United States)

    Bolton, F J; Hutchinson, D N

    1989-01-01

    A surface antigen (SA), acid glycine extract (AGE), and urease preparation (UP) were evaluated using sera from patients undergoing endoscopy and from subjects with gastric or duodenal ulcers. Sera were tested for the presence of IgG and IgA antibodies by a conventional indirect enzyme linked immunosorbent assay (ELISA). In patients with confirmed Campylobacter pylori associated gastritis, raised IgG antibody titres were indicated by absorbance values of greater than or equal to 500, greater than or equal to 500, and greater than or equal to 1500 for the SA, AGE, and UP, respectively. Corresponding values for the IgA assay were greater than or equal to 500, greater than or equal to 500, and greater than or equal to 1000. The specificity of the IgG assays were 94%, 92%, and 90% for the AGE, SA, and UP, respectively. In contrast, the UP was the most sensitive (97%); the other two antigen preparations gave values of 82%. In the IgA assay the UP showed the greatest specificity (90%) and sensitivity (90%). The predictive value for a true positive for the IgG assay was the same for all antigens (93%), whereas the UP gave a predictive value for a true negative of 96% compared with 79% for the other two antigen preparations. Of the patients with gastric or duodenal ulcers, raised antibody titres to SA were found in 72% (IgG) and 73% (IgA), to AGE in 75% (IgG) and 63% (IgA), and to UP in 77% (IgG) and 75% (IgA). The use of a urease antigen preparation to determine IgG antibody is recommended for screening patients undergoing endoscopy. PMID:2760233

  16. Indirect Air Carrier Certification

    Data.gov (United States)

    Department of Homeland Security — Required information is entered in order to obtain an IAC certification and do business as an IAC. Information is verified through site inspections and inspections...

  17. Renewable Energy Certificates (RECs)

    Science.gov (United States)

    Renewable Energy Certificates (RECs), are tradable, non-tangible energy commodities in the United States that represent proof that 1 megawatt-hour (MWh) of electricity was generated from an eligible renewable energy resource.

  18. Preparation and evaluation of transdermal drug delivery system of etoricoxib using modified chitosan

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    Wahid A

    2008-01-01

    Full Text Available In the present investigation chitosan has been chemically modified by treating with two different aldehydes like acetaldehyde and propionaldehyde to form Schiff′s bases. Schiff′s bases of chitosan with acetaldehyde and propionaldehyde were named as polymer A and polymer B, respectively. Fourier Transform Infra Red (FTIR spectral data have confirmed the reaction carried out on chitosan. Drug free polymeric films of chitosan, chemically modified chitosan and chitosan/hydroxypropylmethylcellulose blend were prepared and evaluated for various physicochemical characters. Further, the films were incorporated with anti-inflammatory drug, etoricoxib using glycerol as plasticizer. The drug loaded films were cross-linked with sodium citrate and studied for permeation characteristics across dialysis membrane and rat skin. All the films were evaluated for bursting strength, swelling index, moisture uptake, thickness uniformity, drug content uniformity, tensile strength, percent elongation at break, percent flatness, water vapour transmission rate and in vitro drug permeation study.

  19. Preparation and characterization of starch-metal silicate Co-precipitates--evaluation as tablet superdisintegrant.

    Science.gov (United States)

    Singh, Inderbir; Kaur, Birender; Juneja, Prateek

    2014-01-01

    Starch is a potential biomaterial used for various pharmaceutical applications because of its unique physicochemical and functional characteristics. A number of modification techniques, such as physical, chemical, enzymatic and genetic or a combination of any of these methods have been reported with the aim of enhancing the positive attributes and eliminating the shortcomings of the native starches. The present studies deal with the development of co-precipitates of corn starch with different silicates (Mg, Ca, Al) with an aim of using it as a tablet superdisintegrant. Co-precipitates of starch with different silicates were prepared and FTIR-ATR, XRD and SEM techniques were used for the characterization of conjugates. The conjugate were analyzed for various powder evaluation test like angle of repose, bulk density, tapped density, Hausner's ratio, Carr's index, swelling index and effective pore radius. The prepared co-precipitates were found to possess good powder flow properties. The swelling and effective pore radius of all co-precipitates (SMgC, SAlC and SCaC) was found in the range between 30-100% and 15.89-21.71 μm respectively. Different ratios of the prepared co-precipitates were used to formulate fast disintegrating tablets. Fast disintegrated tablets formulated using starch silicate conjugates as superdisintegrant were evaluated for diameter, thickness, hardness, friability, tensile strength, in vitro tablet disintegration, water absorption ratio, wetting time and in vitro dissolution studies. The effective pore radius and swelling of the co-precipitates were correlated with the in vitro disintegration, water absorption ratio and wetting time of the tablets. It was concluded that silicated co-precipitates of starch could be used as superdisintegrants in pharmaceutical tablet formulations.

  20. Evaluation of seat and non-seat post preparation design using conventional and computational methods

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    G. Subrata

    2009-12-01

    Full Text Available Background: Design of root canal preparation especially in cervical-third area of the root, is one of many factors involved in the success of post-core restoration. Seat design that is used in Prosthodontics Installation, Faculty of Dentistry, University of Padjadjaran, is in the contrary to minimal preparation design. The root fracture resistance of this design has not been proven yet. Purpose: The aim of this study was to evaluate the root fracture resistance of seat compare to non-seat design, with two different research methods: experimental laboratory and computer simulation with Finite Element Method (FEM. Method: The experimental laboratory investigation used 20 upper central incisors: 10 used seat design and 10 non-seats, with the cast posts cemented in the preparation. The specimens were tested by using Universal Testing Machine with compressive force until the root fracture. The FEM used 2D digital models: seat and non-seat design of maxillary central incisors using a finite element software. The distribution of internal stress caused by static loading 110N at 135° angle with longitudinal axis of the tooth was evaluated. Result: The results of the fracture strength test showed a significant difference (p = 0.05 between the non-seat group (852.27N ± 112.6N and the seat group (495.78N ± 82.90N. The FEM showed a lower stress concentration in non-seat compare to seat group. This study proved that non-seat distributes stress better than seat design. Conclusion: It can be concluded that the FEM confirmed the result of the laboratory method. Stress concentration will cause fracture, therefore root fracture resistance in the non-seat design was higher than the seat design.

  1. Preparation and evaluation of a radiogallium complex-conjugated bisphosphonate as a bone scintigraphy agent

    Energy Technology Data Exchange (ETDEWEB)

    Ogawa, Kazuma, E-mail: kogawa@p.kanazawa-u.ac.jp [Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192 (Japan); Takai, Kenichiro; Kanbara, Hiroya; Kiwada, Tatsuto [Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192 (Japan); Kitamura, Yoji; Shiba, Kazuhiro [Advanced Science Research Center, Kanazawa University, Kanazawa 920-8640 (Japan); Odani, Akira [Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa 920-1192 (Japan)

    2011-07-15

    Introduction: {sup 68}Ga is a radionuclide of great interest as a positron emitter for positron emission tomography (PET). To develop a new bone-imaging agent with radiogallium, 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) was chosen as a chelating site and Ga-DOTA complex-conjugated bisphosphonate, which has a high affinity for bone, was prepared and evaluated. Although we are interested in developing {sup 68}Ga-labeled bone imaging agents for PET, in these initial studies {sup 67}Ga was used because of its longer half-life. Methods: DOTA-conjugated bisphosphonate (DOTA-Bn-SCN-HBP) was synthesized by conjugation of 2-(4-isothiocyanatebenzyl)-1,4,7,10-tetraazacyclododecane-1,4,7, 10-tetraacetic acid to 4-amino-1-hydroxybutylidene-1,1-bisphosphonate (alendronate). {sup 67}Ga-DOTA-Bn-SCN-HBP was prepared by coordination with {sup 67}Ga, and its in vitro and in vivo evaluations were performed. Results: {sup 67}Ga-DOTA-Bn-SCN-HBP was prepared with a radiochemical purity of over 95% without purification. {sup 67}Ga-DOTA-Bn-SCN-HBP had great affinity for hydroxyapatite in binding assay. In biodistribution experiments, {sup 67}Ga-DOTA-Bn-SCN-HBP accumulated in bone rapidly but was hardly observed in tissues other than bone. Pretreatment of an excess amount of alendronate inhibited the bone accumulation of {sup 67}Ga-DOTA-Bn-SCN-HBP. Conclusions: {sup 67}Ga-DOTA-Bn-SCN-HBP showed ideal biodistribution characteristics as a bone-imaging agent. These findings should provide useful information on the drug design of bone imaging agents for PET with {sup 68}Ga.

  2. Preparing pharmacists to deliver a targeted service in hypertension management: evaluation of an interprofessional training program.

    Science.gov (United States)

    Bajorek, Beata V; Lemay, Kate S; Magin, Parker J; Roberts, Christopher; Krass, Ines; Armour, Carol L

    2015-09-28

    Non-adherence to medicines by patients and suboptimal prescribing by clinicians underpin poor blood pressure (BP) control in hypertension. In this study, a training program was designed to enable community pharmacists to deliver a service in hypertension management targeting therapeutic adjustments and medication adherence. A comprehensive evaluation of the training program was undertaken. Tailored training comprising a self-directed pre-work manual, practical workshop (using real patients), and practice scenarios, was developed and delivered by an inter-professional team (pharmacists, GPs). Supported by practical and written assessment, the training focused on the principles of BP management, BP measurement skills, and adherence strategies. Pharmacists' experience of the training (expectations, content, format, relevance) was evaluated quantitatively and qualitatively. Immediate feedback was obtained via a questionnaire comprising Likert scales (1 = "very well" to 7 = "poor") and open-ended questions. Further in-depth qualitative evaluation was undertaken via semi-structured interviews several months post-training (and post service implementation). Seventeen pharmacists were recruited, trained and assessed as competent. All were highly satisfied with the training; other than the 'amount of information provided' (median score = 5, "just right"), all aspects of training attained the most positive score of '1'. Pharmacists most valued the integrated team-based approach, GP involvement, and inclusion of real patients, as well as the pre-reading manual, BP measurement workshop, and case studies (simulation). Post-implementation the interviews highlighted that comprehensive training increased pharmacists' confidence in providing the service, however, training of other pharmacy staff and patient recruitment strategies were highlighted as a need in future. Structured, multi-modal training involving simulated and inter-professional learning is effective in preparing

  3. Preparation, Physicochemical Characterization and Performance Evaluation of Gold Nanoparticles in Radiotherapy

    Directory of Open Access Journals (Sweden)

    Ali Kamiar

    2013-08-01

    Full Text Available Purpose: The aim of the present study was preparation, physicochemical characterization and performance evaluation of gold nanoparticles (GNPs in radiotherapy. Another objective was the investigation of anti-bacterial efficacy of gold nanoparticle against E. coli clinical strains. Methods: Gold nanoparticles prepared by controlled reduction of an aqueous HAuCl4 solution using Tri sodium citrate. Particle size analysis and Transmission electron microscopy were used for physicochemical characterization. Polymer gel dosimetry was used for evaluation of the enhancement of absorbed dose. Diffusion method in agar media was used for investigation of anti-bacterial effect. Results: Gold nanoparticles synthesized in size range from 57 nm to 346 nm by planning different formulation. Gold nanoparticle in 57 nm size increased radiation dose effectiveness with the magnitude of about 21 %. At the concentration of 400 ppm, Nano gold exhibited significant anti-bacterial effect against E. coli clinical strains. Conclusion: It is concluded that gold nanoparticles can be applied as dose enhancer in radiotherapy. The Investigation of anti-bacterial efficacy showed that gold nanoparticle had significant effect against E. coli clinical strains.

  4. Docetaxel-loaded solid lipid nanoparticles: preparation, characterization, in vitro, and in vivo evaluations.

    Science.gov (United States)

    Mosallaei, Navid; Jaafari, Mahmoud Reza; Hanafi-Bojd, Mohammad Yahya; Golmohammadzadeh, Shiva; Malaekeh-Nikouei, Bizhan

    2013-06-01

    The aim of the present study was to prepare, characterize, and evaluate solid lipid nanoparticles (SLNs) containing docetaxel (DTX) to improve the efficacy of this chemotherapeutic agent. SLNs containing DTX (SLN-DTX) were prepared by microemulsion and probe sonication techniques. In vitro cytotoxicity of SLN-DTX compared with Taxotere® (TXT) was evaluated on colorectal (C-26) and malignant melanoma (A-375) cell lines. Cellular uptake experiment was also carried out on C-26 cells. In in vivo tests, tumor inhibitory efficacy and survival were compared with TXT on C-26-implanted BALB/c mice. SLN-DTX particle size was 180 nm and PDI of 0.2 with spherical shape. Encapsulation efficacy was more than 98%. SLN-DTX at concentration of 100 μM caused 100% and 99.9% viability reduction in C-26 and A-375 after 48 and 72 h, respectively. The half maximal inhibitory concentration (IC50 ) of SLN-DTX on C-26 and A-375 was respectively 0.769 and 28.132 μM after 24 h. DTX cell uptake from SLN-DTX was remarkably higher than TXT. SLN-DTX showed better tumor inhibitory efficacy and survival at a dose of 10 mg/kg versus 10 and 20 mg/kg TXT. In conclusion, the results of the present study showed that the efficacy of SLN-DTX was better than TXT in cell-uptake and in vivo experiments.

  5. Preparation and evaluation of vitamin A nanosuspension as a novel ocular drug delivery

    Directory of Open Access Journals (Sweden)

    Abbas Akhgari

    2015-10-01

    Full Text Available Objective(s: The aim of this study was to prepare a nanosuspension formulation as a new vehicle for the improvement of the ocular delivery of vitamin A. Material and Methods: Formulations were designed based on full factorial design. A high pressure homogenization technique was used to produce nanosuspensions. Fifteen formulations were prepared by the use of different combinations of surfactants Tween 80, benzalkonium chloride and Pluronic and evaluated for pH, particle size, entrapment efficiency, differential scanning calorimetry (DSC, stability and drug release.  Also, Draize test was used to evaluate the irritation of rabbit eye by formulations. Results: All formulations showed a small mean size that is well suited for ocular application. Also it was observed that the particle size decreased with increase in the amount of surfactant. Drug entrapment increased with increasing amount of surfactant. It was shown that initial and final drug release can be controlled by the ratio and the total amount of surfactants, respectively. Conclusion: It was concluded that the use of Tween 80 and Pluronic in the formualtions with a proper ratio does not show eye irritation and could be useful to achieve a suitable nanosuspension of vitamin A as a novel ocular delivery system.

  6. Preparation and evaluation of the clinical efficacy and safety of tomato lotion containing lycopene

    Directory of Open Access Journals (Sweden)

    Shahtalebi Mohammad-Ali

    2015-10-01

    Full Text Available Introduction: Skin aging starts at around age of 30 with wrinkling and pigmentations as its mainindicators. Delay and prevention of aging is a major concern for a great number of people. Thepurpose of this research was to develop and evaluate the efficacy of an anti-wrinkle tomato lotion,consisting of 5% w/w tomato powder in an oil-in-water (O/W emulsion.Methods: Different O/W emulsions were prepared and stability tests were done on them. Theformulation that successfully passed the stability tests, was chosen and the Solanum lycopersicumpowder was added to the oily phase of this O/W emulsion. The prepared lotion was evaluated forpharmaceutical tests. In vitro permeation studies were performed to measure permeation throughcellulose acetate membrane by diffusion cell at sink condition. In vivo trial for examination of theanti-wrinkle efficacy of lotion was done on 10 healthy women as case group compared with 10volunteers using the placebo lotion (lotion base without Solanum lycopersicum powder as controlgroup.Results: According to the experimented results on the formulated lotion, the efficient time durationfor lotion effectiveness was 42 days. Tomato powder formulated in base of lotion significantlydecreased wrinkles. Our formulation was compatible with skin and caused no sensitivity reactionin human modelsConclusion: The Lycopene in Solanum lycopersicum in this formulation has anti-aging effect. Thisformulation might be a strong candidate for treatment of skin wrinkles.

  7. MULTISENSOR SYSTEM APPLICATION FOR PREPARATIONS BITTERNESS EVALUATION IN TRADITIONAL CHINESE MEDICINE

    Directory of Open Access Journals (Sweden)

    I.S. Yaroshenko

    2014-07-01

    Full Text Available This paper deals with the study of preparations based on medicinal plants used in traditional Chinese medicine for treatment and prevention of a wide range of diseases. The purpose of this research was evaluation of the capabilities of a multisensor system for instrumental assessment of the samples bitterness. 33 samples of medicinal plants were evaluated by tasters according to bitterness intensity from 0 to 6. Methodology of the analysis was developed and repeated measurements of the samples were performed by multisensor system. Tasters’ assessments were used as reference data while multisensor system calibrating. A regression model built according to these data displayed good correlation of the system response with bitterness perceived by people. The parameters of the regression model give the possibility for concluding that the multisensor system is capable to predict the bitterness of the medicinal plants preparations with average precision equal to ±1 of the reference bitterness scale. Relative error of bitterness determination is 14%, which is a good result for such type of measurements (typical error of the taster’s assessment is, as a rule, in the range of 15-30 %.

  8. Preparation and evaluation of dual-mode floating gastroretentive tablets containing itraconazole.

    Science.gov (United States)

    Kim, Ju-Young; Rhee, Yun-Seok; Park, Chun-Woong; Ha, Jung-Myung; Park, Eun-Seok

    2014-11-01

    The aims of the present study were to prepare new dual-mode floating gastroretentive tablets (DF-GRT) containing itraconazole (ITR) and to evaluate influence of the dosage forms on pharmacokinetic parameters of ITR. The solubility of ITR was enhanced around 200 times (from 1.54 to 248.38 µg/mL) by preparing solid dispersion (SD) with hydroxypropylmethyl cellulose. Buoyancy of DF-GRT containing ITR-SD was established by both camphor sublimation and gas generation. Camphor sublimation decreased density of DF-GRT by making pores in tablet matrix, which led to elimination of lag time for floating. Carbon dioxide generated by sodium bicarbonate and citric acid helped to maintain buoyancy of DF-GRT. Therefore DF-GRT floated on the medium without lag time until disintegrated entirely during in vitro release study. They released 89.11% of the drug at 2 h. Residual camphor was sublimation. The pharmacokinetics of DF-GRT was evaluated in six miniature pigs and compared to immediate release tablets (IRT). Mean AUC ratio of GRT/IRT was 1.36 but there was no statistical difference between AUC values. However delayed tmax, increased MRT and equivalent Cmax of DF-GRT supposed it could be a promising tool for gastroretentive drug delivery system containing ITR.

  9. Evaluation of new injection and cavity preparation model in local anesthesia teaching.

    Science.gov (United States)

    Said Yekta, Sareh; Lampert, Friedrich; Kazemi, Saeid; Kazemi, Reza; Brand, Henk S; Baart, Jacques A; Mazandarani, Mina

    2013-01-01

    The aim of this study was to evaluate a recently developed preclinical injection and cavity preparation model in local anesthesia. Thirty-three dental students administered an inferior alveolar nerve block injection in the model, followed by preparation on a tooth. The injection was evaluated by three observers, and the feedback from the model was registered. After completion of the practical session, the opinion of the dental students was explored with a ten-item questionnaire. Thirty dental students (91 percent) performed the injection correctly according to the feedback of the model, and twenty-eight students (85 percent) did so according to the expert opinion. The agreement between feedback from the training model and the expert opinion was high. The students were very satisfied with the opportunity to practice with the training model, as indicated by the high scores on each item of the questionnaire. These results suggest that use of this preclinical training model in anesthesia teaching may have beneficial effects on the administration of local anesthetics by dental students.

  10. FORMULATION AND EVALUATION OF CREAM PREPARED FROM CROTON SPARSIFLORUS MORONG AND THEIR WOUND HEALING ACTIVITY

    Directory of Open Access Journals (Sweden)

    Mahapatra Annada Prasad

    2012-12-01

    Full Text Available Topical application of cream prepared from the chloroform extract of Croton sparsiflorus Morong were formulated and evaluated for its efficacy and safety. Creams were prepared at different concentrations i.e. 5%, 7.5% and 10% (w/w by fusion method using different excipients. These topical formulations were tested for pH, viscosity, spreadability and drug contents uniformity. The stability study was carried out at 4, 25 and 37 0C. The drug content uniformity of creams was found within the range of 98.09 to 98.21%. All the formulations were evaluated for its acute skin irritancy, wound healing activity in Swiss Albino rats. These formulations did not produce any skin irritation for about a week when applied over the skin. Comparative studies showed that the viscosity of the formulations increases, spreadability decreases and vice versa. From the stability studies, creams showed no changes in properties after keeping at different temperatures for 90 days. All the formulations and the normal chloroform extracts of C. sparsiflorus Morong showed significant (P<0.001 wound healing activity by excision wound model and comparable with that of the reference standards and control bases. The measurement of the wound areas were taken on 3rd, 6th, 9th, 12th, 15th and 18th days and the percentages of wound closures were calculated.

  11. Nutritional and sensory evaluation of nutritious porridge prepared using combinations of soy and sorghum grits.

    Science.gov (United States)

    Khetarpaul, Neelam; Goyal, Rajni; Garg, Renu

    2004-01-01

    A study was conducted to determine the acceptability of porridge developed from various proportions of soy and sorghum. Partially defatted soydhal was soaked in four salt solutions namely, sodium carbonate, sodium bicarbonate, sodium tripolyphosphate and sodium chloride. Soaked dhal was dried and made into grits. Soy and sorghum grits were mixed in three proportions i.e. 10:90, 20:80 and 30:70 to develop nutritious porridge. Porridge prepared from wheat grits served as a control. The sensory evaluation of the developed porridge was done using a 9-point hedonic scale. Porridge prepared using sodium carbonate soaked soydhal was found to fall in the 'like slightly' category whereas other three types of porridges as well as control porridge were 'liked moderately' by the panellists. Nutritional evaluation of the developed porridge demonstrated that protein, fat, crude fibre and ash contents increased significantly (P < 0.05) as the level of soy was increased from 10 to 20 and 30 per cent.

  12. PREPARATION AND IN-VITRO EVALUATION OF MICRO EMULSION OF ANTI-HYPERTENSIVE DRUG: VALSARTAN

    Directory of Open Access Journals (Sweden)

    Nishit Patel et al

    2012-09-01

    Full Text Available Valsartan is an orally administered ACE inhibitor for the treatment of hypertension and cardiac failure, but its solubility and oral bioavailability are poor. The objective of investigation is to formulate a microemulsion drug delivery system of valsartan using minimum surfactant concentration that could improve its solubility and oral bioavailability. Valsartan microemulsion were prepared by Phase-titration method. The composition of optimized formulation consist of Capmul MCM(Oil, Tween 20 (Surfactant,PEG 400(Co-Surfactant and it contains 40 mg of Valsartan.Pseudo-ternary phase diagrams were plotted to check for the micro-emulsification range. Prepared microemulsion formulations were tested for micro emulsifying properties and the resultant microemulsion were evaluated for robustness to dilution, viscosity, drug content, thermodynamic stability studies and in-vitrodissolution. The optimized microemulsion formulation further evaluated for thermodynamic stability studies, particle size distribution, and zeta potential to confirm the stability of the formed Microemulsion. Resultant microemulsion optimized formulation (F2 shows drug release (99.71%, droplet size (36 nm, viscosity (0. 8872 cP, Zetapotenial (-38.8 mV and infinite dilution capability. The formulation was found to show a significant improvement in terms of the drug release with complete release of drug within 80 minutes. Thus, micro emulsifying formulation of valsartan was successfully developed with sustained release.

  13. Formulation and in vitro evaluation of the topical antiageing preparation of the fruit of Benincasa hispida

    Directory of Open Access Journals (Sweden)

    Vidya Sabale

    2011-01-01

    Full Text Available Ageing is the phase of gradual decline of body efficiency and metabolic activities after reaching a maturity stage. Free radicals cause oxidative alterations in collagen, elastin material and changes in membrane characteristics and induce polymerization reactions. Use of topical antioxidants can overcome some of these effects and retard actinic ageing. Herbal products are popular due to their minimum risk of side-effects with maximum efficacy. The present study was undertaken to evaluate the antiageing potential of Benincasa hispida fruit extract as not many scientific studies have been carried out to explore its utility as skin renewal enhancer and as an antioxidant. After removing the outer layer and the seeds, the fruit pulp was dried. The dried fruit pulp was extracted successively with petroleum ether, chloroform, ethyl acetate and methanol by Soxhlation for 2 days. Methanol was recovered under vacuum and a dry extract was obtained (yield 4.2% w/w, which was stored in a desiccator. Suitable topical cream base for effective carriage of fruit extract was developed and its in vitro evaluation for skin renewal activity was tested by application to the stratum corneum of human cadaver skin and by dansyl chloride fluorescence method. The results show that the cream prepared from Benincasa fruit extract may prove as an antiageing preparation and can be used for retarding the symptoms of ageing.

  14. The Certification Bandwagon: Know Where You're Headed before Jumping On.

    Science.gov (United States)

    Barksdale, Susan B.; Lund, Teri B.

    1998-01-01

    Presents results of research focusing on identifying "certification best practices" to provide a framework for business managers and certification practitioners. Describes a 10-step process for evaluating certification programs that takes into consideration four perspectives: audience, learning, business, and financial. (AEF)

  15. Environmental aspects of the forest management certification process

    CSIR Research Space (South Africa)

    Scott

    2000-01-01

    Full Text Available sector forestry, South Africa Environmental Aspects of the Forest Management Certification Process David F. Scott ii Environmental Aspects of the Forest Management Certification Process David F. Scott 2000 A report prepared as part of the South...-Environmentek, London and Pretoria. Contacts for the lead author David Scott, Department of Earth and Environmental Sciences, Okanagan University College, 3333 College Way, Kelowna, British Columbia, Canada V1V 1V7, Tel: (250) 762 5445 (ext 7565), E-mail: dscott...

  16. Preparation and evaluation of a novel therapeutic dairy-based drink for phenylketonuria

    Directory of Open Access Journals (Sweden)

    Ahmed M. Abdel-Salam

    2010-02-01

    Full Text Available Background: People with phenylketonuria need to eat a special diet which contains a low level of phenylalanine. Most of these special diets have high protein levels which contain phenylalanine. Control of phenylalanine levels in the early years of life is crucial and remains important throughout childhood, especially for cognitive function and behavior. Aims: The current study evaluated the biological and sensory properties of a novel dairy-based drink for patients with phenylketonuria (PKU. Methods and Materials: The novel dairy-based drink was prepared by emulsifying corn germ oil with casein glycomacropeptide (GMP solution in milk permeates. The chemical composition and sensory properties of the dairy-based drink were determined. In addition, the dairy-based drink was nutritionally evaluated using patient volunteers. These patients followed a strict diet limiting phenylalanine in their food. Phenylalanine levels were measured before and after three days of consuming the dairy-based drink. Results: The results of the sensory evaluation showed that the product was ranked that there were decreases in “good” and was acceptable by all test panels and volunteers. Serum phenylalanine levels in all volunteers decreased between 30% - 80%. Conclusions: The data obtained from the sensory evaluation and the decreases in serum phenylalanine levels encourage us to utilize this formulated dairy-based drink for therapeutic feeding of PKU patients.

  17. Preparation and evaluation of a novel therapeutic dairy-based drink for phenylketonuria

    Directory of Open Access Journals (Sweden)

    Ahmed M Abdel-Salam

    2010-01-01

    Full Text Available Background: People with phenylketonuria need to eat a special diet which contains a low level of phenylalanine. Most of these special diets have high protein levels which contain phenylalanine. Control of phenylalanine levels in the early years of life is crucial and remains important throughout childhood, especially for cognitive function and behavior. Aims: The current study evaluated the biological and sensory properties of a novel dairy-based drink for patients with phenylketonuria (PKU. Methods and Materials: The novel dairy-based drink was prepared by emulsifying corn germ oil with casein glycomacropeptide (GMP solution in milk permeates. The chemical composition and sensory properties of the dairy-based drink were determined. In addition, the dairy-based drink was nutritionally evaluated using patient volunteers. These patients followed a strict diet limiting phenylalanine in their food. Phenylalanine levels were measured before and after three days of consuming the dairy-based drink. Results: The results of the sensory evaluation showed that the product was ranked that there were decreases in "good" and was acceptable by all test panels and volunteers. Serum phenylalanine levels in all volunteers decreased between 30% - 80%. Conclusions : The data obtained from the sensory evaluation and the decreases in serum phenylalanine levels encourage us to utilize this formulated dairy-based drink for therapeutic feeding of PKU patients.

  18. DB2 9 for Linux, UNIX, and Windows Advanced Database Administration Certification Certification Study Guide

    CERN Document Server

    Sanders, Roger E

    2008-01-01

    Database administrators versed in DB2 wanting to learn more about advanced database administration activities and students wishing to gain knowledge to help them pass the DB2 9 UDB Advanced DBA certification exam will find this exhaustive reference invaluable. Written by two individuals who were part of the team that developed the certification exam, this comprehensive study guide prepares the student for challenging questions on database design; data partitioning and clustering; high availability diagnostics; performance and scalability; security and encryption; connectivity and networking; a

  19. 乳粉中阪崎肠杆菌标准物质的研制%Preparation and Certification of reference material for Enterobacter sakazakii in the milk powder

    Institute of Scientific and Technical Information of China (English)

    陈彬; 郑晶; 黄晓蓉; 吴谦; 张体银; 黄嫦娇; 林杰

    2012-01-01

    建立乳粉中阪崎肠杆菌标准物质的制备方法.对阪崎肠杆菌真空冷冻干燥过程中使用的三种保护剂进行比较,筛选出效果最好的奶液(即脱脂奶粉与无菌水的比例为1∶4)作为保护剂,用于冷冻干燥时提高阪崎肠杆菌活菌存活率.在奶液中添加一定浓度的阪崎肠杆菌,使用真空冷冻干燥技术制备成冻干奶粉样品,测其含菌量,根据测得的菌落数量,按比例加入脱脂奶粉混匀稀释成平板菌落计数为10~15 g-1的标准样品,经真空包装获得2批共300个独立包装的冻干阪崎肠杆菌标准样品(10g/包).经过均匀性与稳定性检验,样品的定值及不确定度评估.结果表明:该标准物质均匀性、稳定性良好,该方法对进一步完善微生物尤其是致病菌标准物质的制备具有参考价值.%The preparation method of the establishment of Enterobacter sakazakii reference material in the milk powder. Compare the three protectant used in the process of the vacuum freeze-drying Enterobacter sakazakii, select the most efficient milk solution (i.e. skimmed milk powder and sterile water ratio of 1:4) as a protectant, use it in the vacuum freeze-drying process to improve the survival rate of Enterobacter sakazakii. Add the certain amount of Enterobacter sakazakii to the milk soludon; use the vacuum freeze-drying technique to make frozen-dried powder samples. Check the content of the bacterium, According to the measurement of bacteria content, by a ratio of blend in the skimmed milk powder to make the desired reference sample (10~15 g-1). Make two batches of 300 individual vacuum packed reference frozen Enterobacter sakazakii samples (10 g/bag). After testing the uniformity and stability and certification and uncertainty assessment for the samples, it comes to a conclusion: the reference material has the great uniformity and the good stability, this method has the great value in improving preparation of the microbial especially

  20. ENVIRONMENTAL MANAGEMENT SYSTEMS CERTIFICATION

    Directory of Open Access Journals (Sweden)

    Aniko Miler-Virc

    2012-12-01

    Full Text Available ISO 14001 prescribes the requirements for a system, not environmental performance itself. Similarly, certification is of the management system itself, not environmental performance. An audit is not conducted to ascertain whether your flue gas emissions are less than X part per million nitrous oxide or that your wastewater effluent contains less that Y milligrams of bacteria per litre. Consequently, the procces of auditing the system for compliance to the standard entails checking to see that all of the necessary components of a functioning system are present and working properly.           A company can have a complete and fully functional EMS as prescribed by ISO 14001 without being certified. As certification can add to the time and expense of EMS development, it is important for you to establish, in advance, whether certification is of net benefit to you. Although most companies that develop an EMS do in fact certify, there are cases where certification does not add immediate value. Certification is not always beneficial to small and medium sized companies. Certification is not always necessary for companies with one or two large clients with environmental demands who are satisfied that you have a functional EMS (second-party declaration. Whatever decision you make, it is important to remember that just as a driver′s licence does not automatically make you a good driver, ISO 14001 certification does not automatically make your company environmentally benign or ensure that you will continually improve environmental performance. The system is only as good as the people who operate it.

  1. Preparation and in vitro/in vivo evaluation of resveratrol-loaded carboxymethyl chitosan nanoparticles.

    Science.gov (United States)

    Zu, Yuangang; Zhang, Yin; Wang, Weiguo; Zhao, Xiuhua; Han, Xue; Wang, Kunlun; Ge, Yunlong

    2016-01-01

    Resveratrol (RES) is natural polyphenol with a strong biological activity, but its disadvantages, such as poor water solubility, susceptibility to oxidative decomposition and rapid metabolism in the body, which substantially restricts in vivo bioavailability, need to be resolved. This study used carboxymethyl chitosan (CMCS) as a drug carrier and utilized emulsion cross-linking to prepare RES-loaded CMCS nanoparticles (RES-CMCSNPs). A single-factor experiment was performed to optimize the preparation of these particles; in vitro and in vivo characteristics were evaluated. Spherical RES-CMCSNPs were prepared under optimal conditions, in which average particle size, potential, drug loading and encapsulation efficiency were (155.3 ± 15.2) nm, (-10.28 ± 6.4) mV, (5.1 ± 0.8)% and (44.5 ± 2.2)%, respectively. FTIR, DSC and XRD showed that RES molecules were wrapped in the nanoparticles. In vitro DPPH radical scavenging abilities showed RES-CMCSNPs were better than RES raw powder. The nanoparticles improved the solubility of RES, thereby greatly improving the antioxidant activity of the drug. In vitro release experiments of RES and RES-CMCSNPs by simulating the human gastrointestinal tract were performed, in which RES-CMCSNPs rendered better releasing effects than raw RES. Raw RES and RES-CMCSNPs results were in line with those obtained for the single-chamber model for pharmacokinetic studies in rats. Compared with the bulk drugs, the RES-CMCSNPs exhibited increased in vivo absorption, prolonged duration of action and increased relative bioavailability by 3.516 times more than those of the raw RES. In addition, the residual chloroform is less than the ICH limit for class 2 solvents.

  2. Preparation and evaluation of alginate nanoparticles containing pertussis toxin as a particulate delivery system

    Directory of Open Access Journals (Sweden)

    Hamid Reza Goudarzi

    2016-04-01

    Full Text Available During the last decades, research on different kinds of nanoparticles (NPs has been increased to prepare various medical applications, for instance in vaccine and gene delivery and as new generation of adjuvant candidates. The aim of the present study was to prepare sodium alginate nanoparticles (Alg-NPs containing pertussis toxin (PTX as an candidate acellular vaccine. Formulation of antigen loaded Alg-NPs were assessed for immunological activities and their role as potential immunological adjuvant. Alg-Nps were prepared using mild ionic gelation method. Optimal formulation was obtained by concentration of 0.2%w/v sodium alginate, 0.1%w/v CaCl2 solution and magnetically homogenization condition of 45 min and rate of 2000 rpm. Obtained Alg-NPs showed average size of 88 nm and zeta potential of -32mV in blank and 72 nm and -29 mV in PTX entrapped nanoparticles, respectively. A Loading efficiency of more than 90% was determined for PTX. The antigen loaded nanoparticles showed 75.3% of release within 144 h in in vitro release studies. The immunological evaluation in female Balb/c mice groups revealed that the Alg-NPs formulation induced significantly higher serum antibody titers (p < 0.01 as compared with commercial acellular pertussis vaccine and conventional alum-adjuvanted antigen administered by subcutaneous route. The results showed the potential of Alg-NPS to be a simplex and efficient delivery system. This study also indicated the potential of Alg-NPs as new generation of immunostimulant adjuvant to boost the antigenecity of the antigens in a cellular pertussis vaccines.

  3. Preparation of mPEG2000-PLA-modified Xingnaojing microemulsion and evaluation in mucosal irritation.

    Science.gov (United States)

    Wen, Ran; Li, Huiyun; Du, Shouying; Zhao, Xuejiao; Zhao, Zhuang; Bai, Jie; Lu, Yang

    2014-01-01

    Xingnaojing microemulsion (XNJ-M) administered intranasally is used for stroke treatment. Methoxy poly(ethylene glycol)-poly(lactide) (mPEG-PLA) is a block copolymer with surfactant-like properties and good biodegradability and reliable biological safety. In order to investigate the possibility to lower mucosa irritation with XNJ-M, XNJ-M modified by mPEG2000-PLA (XNJ-MM) was prepared. Different ratios of mPEG2000-PLA were synthesized. The structures and properties were confirmed by 1H-NMR, IR, and DSC. The hydrophile-lipophile balance (HLB) value and critical micellar concentration of copolymers were investigated. The in situ toad palate model was adopted to investigate the ciliotoxicity of the copolymers. The XNJ-M and XNJ-MM were prepared by dropping aqueous phase method. Mucosal irritation of different Xingnaojing (XNJ) preparations was studied by behavioral observations including sneezing and scratching nose. The epithelial thickness of nasal mucosa was evaluated and the secretory protein concentration was determined. The results of in situ toad palate model demonstrated that high HLB value mPEG2000-PLA basically showed no ciliotoxicity, while EL-35 had significant dose-dependent ciliotoxicity (PPLA were significantly lower than EL-35 after seven days of treatment, based on the epithelial thickness of 0.429±0.100 mm and 0.700±0.035 mm, respectively (PPLA-modified microemulsion is a promising dosage form of XNJ, based on reduced irritation on nasal mucosa.

  4. Preparation of Orthosiphon stamineus enriched-extracts and evaluation of their free radical scavenging activity

    Science.gov (United States)

    Mansor, Che Nurul Ain Nadirah Che; Latip, Jalifah; Markom, Masturah

    2016-11-01

    Orthosiphon stamineus is one of the important herbal plants used in folk medicine to cure variety of diseases. Three compounds namely rosmarinic acid (RA), sinensetin (SEN) and eupatorin (EUP) were identified as the bioactive markers. However, a standardized extraction method for the preparation of O. stamineus extract enriched with the bioactive compounds was still undiscovered. Thus, this study aims to establish the optimal extraction method that can be used to prepare the enriched extract with anti-oxidant property. Maceration, reflux and Soxhlet were the extraction methods employed, with ethanol, 50% (v/v) aqueous ethanol and water were chosen as the solvents. Each extracts were evaluated for their biomarker contents (RA, SEN and EUP) and anti-oxidant capacity using thin layer chromatography (TLC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay respectively. Among the three extraction methods employed, the highest total extraction yield was obtained from reflux (72.73%) followed by Soxhlet (62.51%) and maceration (37.78%). Although all extracts found to contain the three biomarkers via TLC visualization analysis, there was variation in the extracts' anti-oxidant capacity, ranging from 6.17% to 72.97%. The variation was expected to be due to the difference in the quantity of the biomarkers in each extract. Furthermore, the anti-oxidative potency of the RA was found comparable to natural anti-oxidant vitamin C, and higher than the synthetic anti-oxidant butylated hydroxyl toluene (BHT). These preliminary results may serve as a starting point towards the preparation of standardized bioactive O. stamineus extract.

  5. Preparation and Evaluation of Tretinoin Microemulsion Based on Pseudo-Ternary Phase Diagram

    Directory of Open Access Journals (Sweden)

    Fatemeh Leis

    2012-06-01

    Full Text Available Purpose: The aim of the present research was to formulate a transparent microemolsion as a topical delivery system for tretinoin for the treatment of acne. Methods: Microemulsion formulations prepared by mixing appropriate amount of surfactant including Tween 80 and Labrasol, co-surfactant such as propylene glycol (PG and oil phase including isopropyl myristate – transcutol P (10:1 ratio. The prepared microemolsions were evaluated regarding their particle size, zeta potential, conductivity, stability, viscosity, differential scanning calorimetry (DSC, scanning electron microscopy (SEM, refractory index (RI and pH. Results: The results showed that maximum oil was incorporated in microemolsion system that was contained surfactant to co-surfactant ratio (Km of 4:1. The mean droplets size range of microemulsion formulation were in the range of 14.1 to 36.5 nm and its refractory index (RI and pH were 1.46 and 6.1, respectively. Viscosity range was 200-350 cps. Drug release profile showed 49% of the drug released in the first 8 hours of experiment belong to ME-7. Also, Hexagonal and cubic structures were seen in the SEM photograph of the microemulsions. Conclusion: physicochemical properties and in vitro release were dependent upon the contents of S/C, water and, oil percentage in formulations.Also, ME-7 may be preferable for topical tretinoin formulation.

  6. Preparation and Evaluation of Gelatin-Chitosan-Nanobioglass 3D Porous Scaffold for Bone Tissue Engineering

    Directory of Open Access Journals (Sweden)

    Kanchan Maji

    2016-01-01

    Full Text Available The aim of the present study was to prepare and characterize bioglass-natural biopolymer based composite scaffold and evaluate its bone regeneration ability. Bioactive glass nanoparticles (58S in the size range of 20–30 nm were synthesized using sol-gel method. Porous scaffolds with varying bioglass composition from 10 to 30 wt% in chitosan, gelatin matrix were fabricated using the method of freeze drying of its slurry at 40 wt% solids loading. Samples were cross-linked with glutaraldehyde to obtain interconnected porous 3D microstructure with improved mechanical strength. The prepared scaffolds exhibited >80% porosity with a mean pore size range between 100 and 300 microns. Scaffold containing 30 wt% bioglass (GCB 30 showed a maximum compressive strength of 2.2±0.1 MPa. Swelling and degradation studies showed that the scaffold had excellent properties of hydrophilicity and biodegradability. GCB 30 scaffold was shown to be noncytotoxic and supported mesenchymal stem cell attachment, proliferation, and differentiation as indicated by MTT assay and RUNX-2 expression. Higher cellular activity was observed in GCB 30 scaffold as compared to GCB 0 scaffold suggesting the fact that 58S bioglass nanoparticles addition into the scaffold promoted better cell adhesion, proliferation, and differentiation. Thus, the study showed that the developed composite scaffolds are potential candidates for regenerating damaged bone tissue.

  7. PEGylation of carbon nanotubes via mussel inspired chemistry: Preparation, characterization and biocompatibility evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Xiaoyong; Zeng, Guangjian; Tian, Jianwen; Wan, Qing; Huang, Qiang [Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031 (China); Wang, Ke; Zhang, Qingsong [Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084 (China); Liu, Meiying; Deng, Fengjie [Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031 (China); Wei, Yen, E-mail: xiaoyongzhang1980@gmail.com [Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084 (China)

    2015-10-01

    Graphical abstract: Water dispersible and biocompatible PEGylated carbon nanotubes were prepared via a novel mussel inspired strategy for the first time. - Highlights: • Surface modification of CNTs via bioinspired chemistry. • CNTs with high water dispersibility and excellent biocompatibility. • PEGytion of CNTs via Michael addition reaction. • Preparation of aminated PEG molecules via chain transfer polymerization. - Abstract: A novel strategy for surface modification of multi-walled carbon nanotubes (MWCNT) was developed via combination of mussel inspired chemistry and Michael addition reaction. In this procedure, pristine MWCNT were first coated with polydopamine (PDA) through self polymerization of dopamine. The PDA functionalized CNT (CNT-PDA) were further functionalized with amino-terminated polymers (polyPEGMA), which were synthesized via free radical polymerization using cysteamine hydrochloride as the chain transfer agent and poly(ethylene glycol) monomethyl ether methacylate as the monomer. The successful modification of CNT was ascertained by a series of characterization techniques including transmission electron microscopy, Fourier transform infrared spectroscopy, thermal gravimetric analysis and X-ray photoelectron spectrometry. The polymer modified CNT showed enhanced dispersibility in aqueous and organic solution. Cytotoxicity evaluation of polymers modified CNT showed that these modified CNT are biocompatible with cells. Finally, due to the universal adhesive of PDA and chain transfer free radical polymerization, this strategy developed in this work can also be extended for surface modification of many other nanomaterials with different functional polymers.

  8. Preparation and Evaluation of Multi-Layer Anodes of Solid Oxide Fuel Cell

    Science.gov (United States)

    Santiago, Diana; Farmer, Serene C.; Setlock, John A.

    2012-01-01

    The development of an energy device with abundant energy generation, ultra-high specific power density, high stability and long life is critical for enabling longer missions and for reducing mission costs. Of all different types of fuel cells, the solid oxide fuel cells (SOFC) is a promising high temperature device that can generate electricity as a byproduct of a chemical reaction in a clean way and produce high quality heat that can be used for other purposes. For aerospace applications, a power-to-weight of (is) greater than 1.0 kW/kg is required. NASA has a patented fuel cell technology under development, capable of achieving the 1.0 kW/kg figure of merit. The first step toward achieving these goals is increasing anode durability. The catalyst plays an important role in the fuel cells for power generation, stability, efficiency and long life. Not only the anode composition, but its preparation and reduction are key to achieving better cell performance. In this research, multi-layer anodes were prepared varying the chemistry of each layer to optimize the performance of the cells. Microstructure analyses were done to the new anodes before and after fuel cell operation. The cells' durability and performance were evaluated in 200 hrs life tests in hydrogen at 850 C. The chemistry of the standard nickel anode was modified successfully reducing the anode degradation from 40% to 8.4% in 1000 hrs and retaining its microstructure.

  9. Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum

    Directory of Open Access Journals (Sweden)

    Boyan Liu

    2016-12-01

    Full Text Available Eupatorium adenophorum is widely distributed throughout the world’s tropical and temperate regions. It has become a harmful weed of crops and natural environments. Its leaves contain bioactive compounds such as chlorogenic acid and may be used as feed additives. In this study, chlorogenic acid was extracted and separated from leaves of E. adenophorum. Three chlorogenic acid products were prepared with different purities of 6.11%, 22.17%, and 96.03%. Phytochemical analysis demonstrated that the main toxins of sesquiterpenes were almost completely removed in sample preparation procedure. The three products were evaluated for safety via in vitro and in vivo toxicological studies. All the products exhibited no cytotoxic effects at a dose of 400 μg/mL in an in vitro cell viability assay. When administered in vivo at a single dose up to 1.5 g/kg bw, all three products caused no signs or symptoms of toxicity in mice. These results encourage further exploration of extracts from E. adenophorum in feed additive application.

  10. Preparation and evaluation of novel octylmethoxycinnamate-loaded solid lipid nanoparticles.

    Science.gov (United States)

    Liu, X-h; Liang, X-z; Fang, X; Zhang, W-P

    2015-08-01

    Octylmethoxycinnamate (OMC)-loaded solid lipid nanoparticles (SLNs) were prepared by ultrasonic emulsification method. Effects of process variables and formulation composition were investigated on particle size and polydispersity index (PI), and the UV absorbance. Effect of OMC concentration on entrapment efficiency (EE) was also studied. The optimal formulation was characterized and evaluated by environment emission scanning electron microscopy (ESEM), differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FT-IR). In addition, the degradations of OMC from SLNs and OMC conventional emulsion were compared. The composition of optimal formulation was determined as 5% (w/w) of solid lipid, 7% (w/w) of emulsifier and 9% of loaded OMC, resulting in a particle size of 392.8 nm, and EE of 88.73%, LD of 38.05% under the preparation condition of 6 min of sonication, 400 W of sonication power. ESEM study showed spherical particles with smooth surface. DSC studies indicated OMC encapsulation within the nanoparticle matrix. The characteristic peaks for OMC-SLNs stood at 1710, 1604, 1513, 1465 and 830.3 cm(-1) . The degradation rate of OMC was decreased when using SLNs formulations compared to conventional emulsion. Hence, the developed SLNs can be used as sunscreen carrier for improve the stability. © 2015 Society of Cosmetic Scientists and the Société Française de Cosmétologie.

  11. Preparation of cross-linked carboxymethyl jackfruit starch and evaluation as a tablet disintegrant.

    Science.gov (United States)

    Kittipongpatana, Nisit; Suwakon, Janta; Kittipongpatana, Ornanong

    2011-10-01

    The main purposes of this study are to prepare cross-linked carboxymethyl jackfruit starch (CL-CMJF) and to evaluate its pharmaceutical property as a tablet disintegrant. CL-CMJF was prepared by a dual carboxymethyl-crosslinking reaction in a flask containing jackfruit seed starch (JFS), chloroacetic acid (CAA), sodium hydroxide (NaOH) and sodium trimetaphosphate (STMP). The reaction was carried out using methanol as a solvent for 60 min at 70°C and at JFS:CAA:NaOH:STMP ratio of 1.0:0.29:0.28:0.07. The obtained CL-CMJF, with degree of substitution and degree of crosslinking calculated to be 0.34 and 0.06, respectively, was insoluble but swellable in water. Rheological study revealed a decreased in solution viscosity compared to the non-crosslinked CMJF. The water uptake of CL-CMJF was 23 times higher than that of native starch and was comparable to that of a commercial superdisintegrant, sodium starch glycolate (SSG). The swelling ability of CL-CMRS was similar to that of crosscarmellose sodium (CCS), another commercial superdisintegrant. Disintegration test of aspirin tablets containing 2%w/w of JFS, CL-CMJF, SSG and CCS showed disintegration times in the order of SSG < CCS ~ CL-CMJF < JFS. The results suggested that CL-CMJF could be developed as a tablet disintegrant.

  12. [Evaluation of the treatment effectiveness of domestic G-SCF preparations in experiments on irradiated dogs].

    Science.gov (United States)

    Rozhdestvenskiĭ, L M; Shliakova, T G; Shchegoleva, R A; Lisina, N I; Zorin, V V

    2013-01-01

    We have evaluated the treatment effectiveness of Leucostim and Neupomax in dogs exposed to radiation at lethal doses of 3 and 3.5 Gy, correspondingly, by testing the dynamics of the blood cell number, first of all, leucocytes and neutrophiles, and the 45-day survival. Supportive therapy for all the dogs, including the control ones, consisted in antibiotic treatment during the acute period of 7-24 days. It was shown that both pre-parations administered consecutively for about 17-21 days after irradiation positively influenced the dynamics of all blood cells but predominantly impacted the neutrophile number dynamics. The latter ones manifested a higher nadir level and an earlier onset of restoration in the G-SCF treated dogs in comparison with the control ones. The tendency to a positive influence on the survival has been shown in Neupomax-treated dogs exposed to 3.5 Gy of radiation (plus about 40%). The results of the experiments were in good accordance with the data by foreign authors who used Neupogen. This allows a conclusion that home-produced G-SCF preparations can replace their foreign analogues.

  13. Preparation and evaluation of cerium oxide-bovine hydroxyapatite composites for biomedical engineering applications.

    Science.gov (United States)

    Gunduz, O; Gode, C; Ahmad, Z; Gökçe, H; Yetmez, M; Kalkandelen, C; Sahin, Y M; Oktar, F N

    2014-07-01

    The fabrication and characterization of bovine hydroxyapatite (BHA) and cerium oxide (CeO2) composites are presented. CeO2 (at varying concentrations 1, 5 and 10wt%) were added to calcinated BHA powder. The resulting mixtures were shaped into green cylindrical samples by powder pressing (350MPa) followed by sintering in air (1000-1300°C for 4h). Density, Vickers microhardness (HV), compression strength, scanning electron microscopy (SEM) and X-ray diffraction (XRD) studies were performed on the products. The sintering behavior, microstructural characteristics and mechanical properties were evaluated. Differences in the sintering temperature (for 1wt% CeO2 composites) between 1200 and 1300°C, show a 3.3% increase in the microhardness (564 and 582.75HV, respectively). Composites prepared at 1300°C demonstrate the greatest compression strength with comparable results for 5 and 10wt% CeO2 content (106 and 107MPa) which are significantly better than those for 1wt% and those that do not include any CeO2 (90 and below 60MPa, respectively). The results obtained suggest optimal parameters to be used in preparation of BHA and CeO2 composites, while also highlighting the potential of such materials in several biomedical engineering applications.

  14. Thiolated chitosan nanoparticles for enhancing oral absorption of docetaxel: preparation, in vitro and ex vivo evaluation

    Science.gov (United States)

    Saremi, Shahrooz; Atyabi, Fatemeh; Akhlaghi, Seyedeh Parinaz; Ostad, Seyed Nasser; Dinarvand, Rassoul

    2011-01-01

    The aim of this study was to prepare and evaluate mucoadhesive core-shell nanoparticles based on copolymerization of thiolated chitosan coated on poly methyl methacrylate cores as a carrier for oral delivery of docetaxel. Docetaxel-loaded nanoparticles with various concentrations were prepared via a radical emulsion polymerization method using cerium ammonium nitrate as an initiator. The physicochemical properties of the obtained nanoparticles were characterized by: dynamic light-scattering analysis for their mean size, size distribution, and zeta potential; scanning electron microscopy and transmission electron microscopy for surface morphology; and differential scanning calorimetry analysis for confirmation of molecular dispersity of docetaxel in the nanoparticles. Nanoparticles were spherical with mean diameter below 200 nm, polydispersity of below 0.15, and positive zeta potential values. The entrapment efficiency of the nanoparticles was approximately 90%. In vitro release studies showed a sustained release characteristic for 10 days after a burst release at the beginning. Ex vivo studies showed a significant increase in the transportation of docetaxel from intestinal membrane of rat when formulated as nanoparticles. Cellular uptake of nanoparticles was investigated using fluoresceinamine-loaded nanoparticles. Docetaxel nanoparticles showed a high cytotoxicity effect in the Caco-2 and MCF-7 cell lines after 72 hours. It can be concluded that by combining the advantages of both thiolated polymers and colloidal particles, these nanoparticles can be proposed as a drug carrier system for mucosal delivery of hydrophobic drugs. PMID:21289989

  15. Proliposomes for oral delivery of dehydrosilymarin: preparation and evaluation in vitro and in vivo

    Institute of Scientific and Technical Information of China (English)

    Chang CHU; Shan-shan TONG; Ying XU; Li WANG; Min FU; Yan-ru GE; Jiang-nan YU; Xi-ming XU

    2011-01-01

    Aim: To formulate proliposomes with a polyphase dispersed system composed of soybean phospholipids, cholesterol, isopropyl myristate and sodium cholate to improve the oral bioavailability of dehydrosilymarin, an oxidized form of herbal drug silymarin.Methods: Dehydrosilymarin was synthesized from air oxidation of silymarin in the presence of pyridine, and proliposomes were prepared by a film dispersion-freeze drying method. Morphological characterization of proliposomes was observed using a transmission electron microscope. Particle size and encapsulation efficiency of proliposomes were measured. The in vitro release of dehydrosilymarin from suspension and proliposomes was evaluated. The oral bioavailability of dehydrosilymarin suspension and proliposomes was investigated in rabbits.Results: The proliposomes prepared under the optimum conditions were spherical and smooth with a mean particle size in the range of 7 to 50 nm. Encapsulation efficiency was 81.59%+0.24%. The in vitro accumulative release percent of dehydrosilymarinloaded proliposomes was stable, which was slow in pH 1.2, and increased continuously in pH 6.8, and finally reached 86.41% at 12 h. After oral administration in rabbits, the relative bioavailability of proliposomes versus suspension in rabbits was 228.85%.Conclusion: Proliposomes may be a useful vehicle for oral delivery of dehydrosilymarin, a drug poorly soluble in water.

  16. The Perceived Benefits of a Preparing Future Faculty Program and Its Effect on Job Satisfaction, Confidence, and Competence

    Science.gov (United States)

    Wurgler, Emily; VanHeuvelen, Jane S.; Rohrman, Shawna; Loehr, Annalise; Grace, Matthew K.

    2014-01-01

    The training of effective instructors and future faculty members is a critical component of doctoral programs in sociology. Many universities and departments have instituted a single course, course sequence, or certification program dedicated to the preparation of future academic faculty. This article evaluates the efficacy of one such program,…

  17. Chemometric evaluation of physicochemical properties of carbonated-apatitic preparations by Fourier transform infrared spectroscopy.

    Science.gov (United States)

    Otsuka, Makoto; Papangkorn, Kongnara; Baig, Arif A; Higuchi, William I

    2012-08-01

    The purpose of this study was to develop a simple and quick method of evaluating the physicochemical properties of carbonated apatite preparations (CAP) as an index of the bioaffinity of implantable materials based on Fourier-transformed-infrared (IR) spectra by chemometrics. The wet-synthesized CAPs contained various levels of carbonate content (CO(3)), and were analyzed microstrain parameter (MS), crystallite size parameter (CP), specific surface area (Sw), CO(3), and solubility parameter (pK(HAP)) using by X-ray powder diffraction, nitrogen gas adsorption, IR, and UV absorption. The IR spectral results of CAPs suggested that the peak intensities of CAP reflected the physicochemical properties of the samples. The IR data sets were calculated to obtain calibration models evaluating the physicochemical properties of CAPs by a partial least squares regression analysis (PLS). As validation of the calibration model, physicochemical properties of CAP could be evaluated based on validation IR data sets of independent samples, and those values had sufficient accuracy. The regression vector of each calibration model suggested that the physicochemical properties of CAP, such as CO(3), Sw, MS, CP, and pK(HAP), were affected by phosphate, hydroxyl, and carbonate groups.

  18. Evaluation of bioactive properties and phenolic compounds in different extracts prepared from Salvia officinalis L.

    Science.gov (United States)

    Martins, Natália; Barros, Lillian; Santos-Buelga, Celestino; Henriques, Mariana; Silva, Sónia; Ferreira, Isabel C F R

    2015-03-01

    The therapeutic benefits of medicinal plants are well known. Nevertheless, essential oils have been the main focus of antioxidant and antimicrobial studies, remaining scarce the reports with hydrophilic extracts. Thus, the antioxidant and antifungal activities of aqueous (prepared by infusion and decoction) and methanol/water (80:20, v/v) extracts of sage (Salvia officinalis L.) were evaluated and characterised in terms of phenolic compounds. Decoction and methanol/water extract gave the most pronounced antioxidant and antifungal properties, being positively related with their phenolic composition. The highest concentration of phenolic compounds was observed in the decoction, followed by methanol/water extract and infusion. Fungicidal and/or fungi static effects proved to be dependent on the extracts concentration. Overall, the incorporation of sage decoction in the daily diet or its use as a complement for antifungal therapies, could provide considerable benefits, also being an alternative to sage essential oils that can display some toxic effects.

  19. Evaluation of the Properties of Bituminous Concrete Prepared from Brick-Stone Mix Aggregate

    Directory of Open Access Journals (Sweden)

    Dipankar Sarkar

    2016-01-01

    Full Text Available The paper describes an investigation into mechanical properties of brick-stone bituminous concrete mix. The effect of brick-stone mix on various mechanical properties of the bituminous concrete such as Marshall stability, flow, Marshall Quotient (stability to flow ratio, Indirect Tensile Strength, stripping, rutting, and fatigue life of bituminous concrete overlay has been evaluated. In this study over-burnt brick aggregate (OBBA and stone aggregate (SA have been mixed in different ratios (by weight such as 20 : 80, 40 : 60, 60 : 40, and 80 : 20, respectively. The laboratory results indicate that bituminous concrete, prepared by 20% brick aggregate and 80% stone aggregate, gives the highest Marshall stability. This bituminous concrete mix shows considerable improvement in various mechanical properties of the mix as compared to the other mixes.

  20. Preparation and evaluation of (99m)Tc-DMSA lyophilized kit for renal imaging.

    Science.gov (United States)

    Jan, Syed Umer; Abbass, Hafiz Ghulam

    2013-05-01

    Dimercaptosuccinic acid (DMSA) has been evaluated and used with technetium 99m ((99m)Tc) in imaging of kidneys. DMSA lyophilized kits were prepared and radiolabelled with (99m)Tc. Paper and thin-layer chromatography have been employed using various eluent systems for the radiochemical analysis, percentage labeling and binding capacity of (99m)Tc-DMSA. Female albino rabbits were used for this study. Biological data obtained after intravenous injection of radiolabelled DMSA to female albino rabbits revealed 32.42% uptake and long retention time in the kidneys. On the basis of animal biodistribution data, it is suggested that DMSA when labeled with (99m)Tc is useful complex for renal imaging and can be successfully applied as a diagnostic tool in nuclear medicine. Clinical biodistribution and radiation dosimetry studies are planned in future.

  1. Preparation of Aminated Macroporous Polyvinyl Alcohol Resins and Evaluation for Bilirubin Adsorption

    Institute of Scientific and Technical Information of China (English)

    WANG Wei-chao; ZHANG Sheng-nan; HU Yue-han; XIE Hui; OU Lai-liang; YU Yao-ting; KONG De-ling; GU Han-qing

    2008-01-01

    In the present study we prepared macroporous polyvinyl alcohol beads. A series of bilirubin adsorbents were generated by immobilization of eight amine agents to the beads as ligands. The adsorption of bilirubin was evaluated by in vitro static and dynamic adsorption tests. The results show that these adsorbents have excellent adsorption efficiency and capacity. Among the eight ligands, trimethylamine (TMA), triethylamine (TEA) and 1,6- hexanediamine(HAD) showed the highest adsorption capacity. The adsorption equilibrium can be achieved in half an hour, and the adsorption percentage of bilirubin was up to 80%. Static electricity and hydrophobic interaction played the main role in bilirubin adsorption, and the adsorption was found to match the monolayer model. The excellent adsorption of these adsorbents indicates their potential in clinical treatment.

  2. Efficacy evaluation of activated carbon prepared from date stones in cyanide adsorption from synthetic wastewater

    Directory of Open Access Journals (Sweden)

    Bahman Ramavandi

    2013-02-01

    Results: The maximum adsorption capacity of 50.21 mg/g corresponded with Langmuir model. Kinetic evaluation indicated that the adsorption of cyanide ions by the adsorbent clearly followed the pseudo-second order rate reaction. The absorption was pH dependent and the maximum adsorption was done by solutions having pH 9. At an optimum pH 9, over 97% removal of 100 mg/L cyanide was attained for an adsorbent dose of 1.25 g/L during a 40 min contact time. Conclusion: Activated carbon prepared from date stones is an efficient and low-cost adsorbent for removing of different concentrations of cyanide from water and wastewater.

  3. Evaluation of the nutritional quality of wheat bread prepared with quinoa, buckwheat and pumpkin seed blends

    Directory of Open Access Journals (Sweden)

    Milovanović Mirjana M.

    2014-01-01

    Full Text Available The purpose of this research was to blend quinoa (Chenopodium quinoa Willd., buckwheat (Fagopyrum esculentum Möench and pumpkin (Cucurbita pepo L. seed kernels at 40% level with wheat flour and to examine the effect of this blend on nutritional and sensory quality and also energy values of the pan bread. Hydrothermal preparation of these supplements is included. Chemical composition of the investigated materials, wheat bread and supplemented bread, was determined using relevant AOAC methods. Chemical composition of supplemented bread with an increase in protein, oil and crude fiber was superior in comparison with control wheat bread. Sensory properties of supplemented bread such as specific volume, appearance, crust and crumb texture, aroma-odor and color were evaluated and found excellent. [Projekat Ministarstva nauke Republike Srbije, br. TR 31006, TR 31034 and EU FP7 project 316004, project acronym: AREA

  4. Evaluation of iodine content and stability in recipes prepared with biofortified potatoes.

    Science.gov (United States)

    Cerretani, Lorenzo; Comandini, Patrizia; Fumanelli, Davide; Scazzina, Francesca; Chiavaro, Emma

    2014-11-01

    Iodine is an essential micronutrient of the human diet. Deficiency of iodine is diffused in many areas of the world and mild deficiency is present also in developed countries around Europe. Biofortification of vegetables could represent a better strategy with respect to iodized salt in order to increase iodine intake. The aim of this study was evaluating the stability of iodine, derived from biofortified potatoes, in the preparation process of three Italian typical dishes: dumplings, vegetable pie, and focaccia bread. The obtained results showed a good stability of iodine in cooking processes, in particular, during baking of focaccia bread. Significant losses of iodine were detected during boiling of dumplings and baking of vegetable pie. Although the different stability during the cooking processes, the three dishes maintained a good final content of iodine, ranging from the 33.3% to 52.7% of daily recommended intake in adults for individual serving size.

  5. Consensus recommendations on rater training and certification.

    Science.gov (United States)

    West, Mark D; Daniel, David G; Opler, Mark; Wise-Rankovic, Alexandria; Kalali, Amir

    2014-01-01

    There is currently no accepted standard for the clinical research industry to follow when selecting and training raters to administer rating scales in clinical neuroscience trials. This article offers guidelines, based on expert recommendations of the CNS Summit Rater Training and Certification Committee, for selecting, training, and evaluating raters. The article also defines terminology and offers recommendations for considering raters with prior training and certification. These guidelines are intended for investigators, pharmaceutical companies, contract research organizations, and other entities involved in clinical neuroscience trials.

  6. Preparation and in vitro antibacterial evaluation of gatifloxacin mucoadhesive gellan system

    Directory of Open Access Journals (Sweden)

    K Kesavan

    2010-12-01

    Full Text Available "nBackground and the purpose of the study: The poor bioavailability and therapeutic response exhibited by the conventional ophthalmic solutions due to precorneal elimination of the drug may be overcome by the use of mucoadhesive in situ gel forming systems that are instilled as drops into the eye and undergo a sol-gel transition in the cul-de-sac and have good mucoadhesion with ocular mucus layers. The objective of this study was to formulate ophthalmic mucoadhesive system of gatifloxacin (GTN and to evaluate its in vitro antibacterial potential against, Staphylococcus aureus and Escherichia coli. "nMethods: Mucoadhesive systems were prepared using gellan combined with sodium carboxymethylcellulose (NaCMC or sodium alginate to enhance the gel bioadhesion properties.The prepared formulations were evaluated for their gelation, and rheological behaviors, mucoadhesion force, in vitro drug release, and antibacterial activity. "nResults: All formulations in non-physiological or physiological conditions showed pseudoplastic behaviors. Increase in the concentration of mucoadhesive agent enhanced the mucoadhesive force significantly. In vitro release of gatifloxacin from the mucoadhesive system in simulated tear fluid (STF, pH of 7.4, was influenced significantly by the properties and concentration of gellan, sodium carboxymethyl cellulose and sodium alginate. Significant reduction in the total bacterial count was observed between drug solution (control and mucoadhesive batches against both tested organisms. "nMajor conclusion: The developed mucoadhesive system is a viable alternative to conventional eye drops of GTN due to its ability to enhance bioavailability through its longer precorneal residence time and ability to sustain the release of the drug.

  7. Preparation and in vitro evaluation of self-nanoemulsifying drug delivery systems (SNEDDS) containing clotrimazole.

    Science.gov (United States)

    Kassem, A A; Marzouk, M A; Ammar, A A; Elosaily, G H

    2010-10-01

    This study sought to formulate and evaluate a self-nanoemulsified drug delivery system (SNEDDS) for clotrimazole (CT), a poorly water-soluble antimycotic drug, used in vaginal delivery. SNEDDS was developed to increase the CT dissolution rate, solubility, and ultimately bioavailability. The solubility of CT in various oils, surfactants, and co-surfactants was determined. Based on solubility studies, oil phase (oleic acid without or with coconut oil), surfactant (Tween 20), and co-surfactants (PEG 200 and n-butanol) were selected and grouped in two combinations for phase studies. Pseudo-ternary phase diagrams were used to evaluate the area of self-nanoemulsification. Essential properties of the prepared systems with regard to emulsion droplet size and turbidity value were determined. In order to investigate the potential for interaction between any of the SNEDDS ingredients used, FTIR spectroscopy was performed. In vitro release studies were performed with SNEDDS formulations in capsules, and the plain drug served as a control. The droplet size of the nanoemulsion was greatly affected by the ratio of the surfactant and co-surfactant. Based on the results with regard to droplet size, turbidity values, and complete drug release after 3 h, three optimized formulations were selected; each contained oleic acid/coconut oil/Tween 20/PEG 200/n-butanol in ratios of 10:0:60:15:15 (%, w/w), 7.5:2.5:53.5:13.3:13.3 (%, w/w), and 6.7:3.3:60:10:10 (%, w/w), respectively. Results suggested that the prepared SNEDDS formulations produced acceptable properties in terms of immediate drug release and could increase the bioavailability of CT.

  8. Preparation and evaluation of tilmicosin-loaded hydrogenated castor oil nanoparticle suspensions of different particle sizes.

    Science.gov (United States)

    Chen, Xiaojin; Wang, Ting; Lu, Mengmeng; Zhu, Luyan; Wang, Yan; Zhou, WenZhong

    2014-01-01

    Three tilmicosin-loaded hydrogenated castor oil nanoparticle (TMS-HCO-NP) suspensions of different particle sizes were prepared with different polyvinyl alcohol surfactant concentrations using a hot homogenization and ultrasonic technique. The in vitro release, in vitro antibacterial activity, mammalian cytotoxicity, acute toxicity in mice, and stability study were conducted to evaluate the characteristics of the suspensions. The in vitro tilmicosin release rate, antibacterial activity, mammalian cytotoxicity, acute toxicity in mice, and stability of the suspensions were evaluated. When prepared with polyvinyl alcohol concentrations of 0.2%, 1%, and 5%, the mean diameters of the nanoparticles in the three suspensions were 920±35 nm, 452±10 nm, and 151±4 nm, respectively. The three suspensions displayed biphasic release profiles similar to that of freeze-dried TMS-HCO-NP powders, with the exception of having a faster initial release. Moreover, suspensions of smaller-sized particles showed faster initial release, and lower minimum inhibitory concentrations and minimum bactericidal concentrations. Time-kill curves showed that within 12 hours, the suspension with the 151 nm particles had the most potent bactericidal activity, but later, the suspensions with larger-sized particles showed increased antibacterial activity. None of the three suspensions were cytotoxic at clinical dosage levels. At higher drug concentrations, all three suspensions showed similar concentration-dependent cytotoxicity. The suspension with the smallest-sized particle showed significantly more acute toxicity in mice, perhaps due to faster drug release. All three suspensions exhibited good stability at 4°C and at room temperature for at least 6 months. These results demonstrate that TMS-HCO-NP suspensions can be a promising formulation for tilmicosin, and that nanoparticle size can be an important consideration for formulation development.

  9. Preparation, characterization and in vivo evaluation of formulation of repaglinide with hydroxypropyl-β-cyclodextrin.

    Science.gov (United States)

    Liu, Meina; Cao, Wen; Sun, Yinghua; He, Zhonggui

    2014-12-30

    The therapeutic efficacy of repaglinide (RPG) is limited by the low and variable oral bioavailability owing to its limited aqueous solubility. In our present study, the development and evaluation of inclusion complex applying hydroxypropyl-β-cyclodextrin (HP-β-CD) for the improvement of oral bioavailability of repaglinide was investigated systematically. The inclusion complex of repaglinide was prepared by lyophilization technique using drug: hydroxypropyl-β-cyclodextrin (1:15 mole). The prepared complexation was characterized by differential scanning calorimetry (DSC), X-ray diffractometry (XRD), NMR spectroscopy and evaluated by dissolution studies. The (1)H NMR was used in the structure study of repaglinide-HP-β-CD (RPG-HP-β-CD) inclusion complex. The analysis proved the higher probability of the repaglinide A-ring into the narrow rim of the β-cyclodextrin molecule. All the characterization information confirmed the formation of RPG-HP-β-CD inclusion complex. The in vivo pharmacokinetics of RPG-HP-β-CD and their physical mixture were performed in beagle dogs. For the first time, a simple, rapid, and sensitive LC-MS/MS method for determination of RPG in beagle dog plasma was developed. The Cmax and AUC0-t of RPG-HP-β-CD were 2.5 and 2 times higher than that of the physical mixture. These results suggested that the interaction of repaglinide with HP-β-CD could notably improve the dissolution rate and bioavailability of repaglinide comparing with its physical mixture. Copyright © 2014 Elsevier B.V. All rights reserved.

  10. AERA Statement on Use of Value-Added Models (VAM) for the Evaluation of Educators and Educator Preparation Programs

    Science.gov (United States)

    Educational Researcher, 2015

    2015-01-01

    The purpose of this statement is to inform those using or considering the use of value-added models (VAM) about their scientific and technical limitations in the evaluation of educators and programs that prepare teachers. The statement briefly reviews the background and current context of using VAM for evaluations, enumerates specific psychometric…

  11. ECOLOGICAL CERTIFICATION AND LABELLING OF TOURIST SERVICES

    Directory of Open Access Journals (Sweden)

    Mirela Stefanica

    2013-12-01

    Full Text Available This work presents an analysis of ecological certification and labelling in the field of tourism. In a tourist company, the certification schemes evaluate the general approach of environmental problems, and impose to the tourist services the observance of pre-established principles and guiding lines concerning the environment. They become for the tourist companies a behavioural standard in the development of their activity. The requirements within such schemes are often flexible and open to interpretation, and generally less controversial than the eco-labelling schemes. As compared to the certification schemes, the eco-labels attributed to the tourist services emphasise the impact over the environment of the services of tourist companies, on the basis of certain European criteria. These criteria refer to the whole life cycle of these tourist services.

  12. Certificate Revocation Using Fine Grained Certificate Space Partitioning

    Science.gov (United States)

    Goyal, Vipul

    A new certificate revocation system is presented. The basic idea is to divide the certificate space into several partitions, the number of partitions being dependent on the PKI environment. Each partition contains the status of a set of certificates. A partition may either expire or be renewed at the end of a time slot. This is done efficiently using hash chains.

  13. Rehabilitation Counselor Certification: Moving Forward

    Science.gov (United States)

    Saunders, Jodi L.; Barros-Bailey, Mary; Chapman, Cindy; Nunez, Patricia

    2009-01-01

    This article provides a brief history of the Commission on Rehabilitation Counselor Certification and presents recent changes and strategic goals for moving forward. Challenges and opportunities for the profession in relation to certification are also discussed. (Contains 3 tables.)

  14. Evaluation of Er:YAG laser radiation cavity preparation under transmitted and polarized light and by electron microanalyser

    Science.gov (United States)

    Dostalova, Tatjana; Krejsa, Otakar; Rokytova, Kveta; Jelinkova, Helena; Hamal, Karel

    1993-12-01

    The results of evaluation of Er:YAG radiation cavity preparation under transmitted and polarized light and by the electron microanalyzer are presented. We examined the longitudinal sections of intact teeth, teeth with early dental caries, teeth with laser preparation in enamel and teeth with Er:YAG laser preparation in enamel and dentine class I and V. We examined intact teeth and laser prepared teeth for the variation of mineralization. Phosphorus and calcium were regularly distributed. The margins of cavities had no necrosis zones. The defects of mineralization in caries depend on demineralization of hard tissues. In the structures was irregular loss of calcium and phosphorus. Thus, Er:YAG laser irradiation with water cooling has been found to be a non-invasive preparation technique.

  15. Board Certification in Counseling Psychology

    Science.gov (United States)

    Crowley, Susan L.; Lichtenberg, James W.; Pollard, Jeffrey W.

    2012-01-01

    Although specialty board certification by the American Board of Professional Psychology (ABPP) has been a valued standard for decades, the vast majority of counseling psychologists do not pursue board certification in the specialty. The present article provides a brief history of board certification in general and some historical information about…

  16. Board Certification in Counseling Psychology

    Science.gov (United States)

    Crowley, Susan L.; Lichtenberg, James W.; Pollard, Jeffrey W.

    2012-01-01

    Although specialty board certification by the American Board of Professional Psychology (ABPP) has been a valued standard for decades, the vast majority of counseling psychologists do not pursue board certification in the specialty. The present article provides a brief history of board certification in general and some historical information about…

  17. 77 FR 6482 - Conductor Certification

    Science.gov (United States)

    2012-02-08

    ... TRANSPORTATION Federal Railroad Administration 49 CFR Part 242 Conductor Certification AGENCY: Federal Railroad..., published on November 9, 2011, which prescribed regulations for certification of conductors as required by... Certification, U.S. Department of Transportation, Federal Railroad Administration, Mail Stop-25, Room...

  18. Preparation and corrosion behavior evaluation of amalgam/titania nano composite

    Directory of Open Access Journals (Sweden)

    Neda Bahremandi Tolou

    2011-01-01

    Conclusion: By adding nano particles of titania and preparing amalgam/titania nano composite as a dental amalgam, corrosion behavior and mercury release during the 2 st h after preparation could be improved.

  19. Sensory evaluation and nutritional value of cakes prepared with whole flaxseed flour

    Directory of Open Access Journals (Sweden)

    Érica Aguiar Moraes

    2010-12-01

    Full Text Available The objective of this study was to evaluate the nutritional value, the oxidative stabilitiy, and consumer acceptance of cakes containing four different concentrations of flaxseed flour (5, 15, 30 and 45% as partial replacement for wheat flour. The oxidative stability of polyunsaturated fatty acids was evaluated through the lipid peroxidation test (TBARS in the flour and cakes. Linolenic acid was determined by gas chromatography as well as contents of protein, lipid, ash, and dietary fiber. Consumer acceptance was assessed using a structured hedonic scale of nine points. The oxidative stability of lipid flaxseeds was not affected by the heat treatment during flour processing and cake baking. Cakes made with 5, 15, and 30% of flaxseed flour, the most accepted by consumers, had dietary fiber levels ranging from 3.5 to 6.2 g and linolenic acid ranging from 445 to 2,500 mg.100 g-1 of the product. The cakes received claims of good and excellent source of dietary fiber and linolenic acid, respectively, both are bioactive compounds. The use of up to 30% of flaxseed flour in the preparation of cakes is a useful strategy to optimize the consumption of food rich in functional ingredients.

  20. Preparation and in vitro/in vivo evaluation of revaprazan hydrochloride nanosuspension.

    Science.gov (United States)

    Li, Wei; Yang, Yonggang; Tian, Yongshou; Xu, Xinlan; Chen, Yang; Mu, Liwei; Zhang, Yaqiong; Fang, Liang

    2011-04-15

    Revaprazan hydrochloride (RH) is a new reversible proton pump inhibitor. However, due to poor water solubility, oral bioavailability of the drug was relatively low. To investigate the particle size reduction effect of RH on dissolution and absorption, three suspensions that containing different sized particles were prepared by high pressure homogenization and in vitro/in vivo evaluations were carried out. DSC and powder X-ray diffraction were used to study crystalline state of freeze dried powder of RH suspensions and the results showed that particles of RH microsuspension and nanosuspension remained in the same crystalline state as coarse suspension, but had lower lattice energy. In the in vitro dissolution test, both microsuspension and nanosuspension showed increased dissolution rate. In the in vivo evaluation, compared to coarse suspension, RH nanosuspension exhibited significant increase in AUC(0-t), C(max) and decrease in T(max), MRT. Nevertheless, RH microsuspension did not display any significant differences in these pharmacokinetic parameters compared to the coarse suspension. The findings revealed that particle size reduction can influence RH absorption in gastrointestinal tract and nanosuspension can enhance oral bioavailability of RH in rats.

  1. Preparation and in vitro/in vivo evaluation of gestodene (GEST) intravaginal ring.

    Science.gov (United States)

    Li, Chunxiao; Ning, Meiying; Yao, Xiaodong; Wang, Yankun; Liu, Ying

    2016-09-01

    Preparation and in vitro/in vivo evaluation of gestodene (GEST) intravaginal ring (IVR) formulations which can release a constant dose of GEST during 3 weeks were investigated. In present study a reservoir gestodene intravaginal ring, including a gestodene silicone elastomer core and the non-active silicone layer, was reported, which was manufactured by reaction injection moulding at 80°C for 20 min. The raw materials compatibility experiments showed that the silicone elastomer core carrier wouldn't interact with drugs. In vitro release samples were determined by HPLC and the experiment was performed under sink conditions. The equation of cumulative release verse time was Y=64.76χ+5.44 (r=0.9998), performing zero-order release at about the target dose of 60 µg/day over 21 days. Drug release increased with temperature elevating from 45 to 55°C, which could be attributed to optimizing the prescription. In addition, the pharmacokinetic and safety studies of gestodene intravaginal ring were evaluated in female New Zealand White rabbits. The GEST in plasma was analyzed by LC-MS/MS and the results proved that the correlation between in vitro and in vivo was relatively well.

  2. Preparation and evaluation of chitosan/ellagic acid/erythrocyte membrane lipid hemostatic composite sponge

    Institute of Scientific and Technical Information of China (English)

    贺庆; 敖强; 王臻; 刘伟强; 龚锴; 公衍道; 张秀芳

    2013-01-01

    BACKGROUND: Some previous studies have indicated that the hemostatic effect of chitosan is limited when dealing with severe injuries. Therefore, the procoagulant activity of chitosan-based hemostatic agents needs to be enhanced. OBJECTIVE: To prepare a novel chitosan/el agic acid/erythrocyte membrane lipid composite sponge and to evaluate its procoagulant activity and cytotoxicity.METHODS: Chitosan sponge and chitosan acetate sponge were prepared by freeze-drying method. Then chitosan/el agic acid/erythrocyte membrane lipid composite sponge was prepared by electrostatic adsorption method. Procoagulant activity of the chitosan, chitosan acetate, and chitosan/el agic acid/erythrocyte membrane lipid sponges was evaluated by the plasma recalcification time method. Hemostatic effect of these sponges was evaluated in the Sprague Dawley rat liver bleeding model, and the cytotoxicity to L929 cel line was evaluated. RESULTS AND CONCLUSION: The plasma recalcification time, bleeding time and blood loss of the chitosan/el agic acid/erythrocyte membrane lipid composite sponge group were significantly lower than those of the chitosan sponge and chitosan acetate sponge groups (P < 0.01). Cel culture experiment showed that the chitosan/el agic acid/erythrocyte membrane lipid composite sponge did not have cytotoxicity. The results indicate that the chitosan/el agic acid/erythrocyte membrane lipid composite sponge has the enhanced procoagulant activity and non-cytotoxicity.%  背景:部分文献报道壳聚糖对严重创伤的止血效果有限,因此以壳聚糖为基础止血剂的促凝血活性还有待进一步增强。目的:制备一种新型的壳聚糖/鞣花酸/红细胞膜脂复合海绵,评价其促凝血活性和细胞毒性。方法:通过冻干法制备壳聚糖海绵和壳聚糖乙酸盐海绵,然后再通过静电吸附法制备壳聚糖/鞣花酸/红细胞膜脂复合海绵。血浆复钙时间法观察3种海绵的促凝血活性,并检测3

  3. Improving the transition from medical school to internship - evaluation of a preparation for internship course.

    Science.gov (United States)

    Scicluna, Helen A; Grimm, Michael C; Jones, Philip D; Pilotto, Louis S; McNeil, H Patrick

    2014-02-03

    This study evaluates the impact of a new 'Preparation for Internship' (PRINT) course, which was developed to facilitate the transition of University of New South Wales (UNSW) medical graduates from Medical School to Internship. During a period of major curricular reform, the 2007 (old program) and 2009 (new program) cohorts of UNSW final year students completed the Clinical Capability Questionnaire (CCQ) prior to and after undertaking the PRINT course. Clinical supervisors' ratings and self-ratings of UNSW 2009 medical graduates were obtained from the Hospital-based Prevocational Progress Review Form. Prior to PRINT, students from both cohorts perceived they had good clinical skills, with lower ratings for capability in procedural skills, operational management, and administrative tasks. After completing PRINT, students from both cohorts perceived significant improvement in their capability in procedural skills, operational management, and administrative tasks. Although PRINT also improved student-perceived capability in confidence, interpersonal skills and collaboration in both cohorts, curriculum reform to a new outcomes-based program was far more influential in improving self-perceptions in these facets of preparedness for hospital practice than PRINT. The PRINT course was most effective in improving students' perceptions of their capability in procedural skills, operational management and administrative tasks, indicating that student-to-intern transition courses should be clinically orientated, address relevant skills, use experiential learning, and focus on practical tasks. Other aspects that are important in preparation of medical students for hospital practice cannot be addressed in a PRINT course, but major improvements are achievable by program-wide curriculum reform.

  4. Preparation and Evaluation of Newly Developed Chitosan Salt Coating Dispersions for Colon Delivery without Requiring Overcoating.

    Science.gov (United States)

    Yamada, Kyohei; Iwao, Yasunori; Bani-Jaber, Ahmad; Noguchi, Shuji; Itai, Shigeru

    2015-01-01

    Although chitosan (CS) has been recognized as a good material for colon-specific drug delivery systems, an overcoating with an enteric coating polymer on the surface of CS is absolutely necessary because CS is soluble in acidic conditions before reaching the colon. In the present study, to improve its stability in the presence of acid, a newly developed CS-laurate (CS-LA) material was evaluated as a coating dispersion for the development of colon-specific drug delivery systems. Two types of CS with different molecular weights, CS250 and CS600, were used to prepare CS-LA films by the casting method. The CS250-LA films had smooth surfaces, whereas the surfaces of the CS600-LA films were rough, indicating that the CS250-LA dispersion could form a denser film than CS600-LA. Both of these CS-LA films maintained a constant shape over 22 h in a pH 1.2 HCl/NaCl buffer, where the corresponding CS films rapidly disintegrated. In addition, the CS250-LA film showed specific colon degradability in a pH 6.0 phosphate buffered solution containing 1.0% (w/v) β-glucosidase. As a result of tensile strength and elongation at the break, both CS-LA films were found to have flexible film properties. Finally, the release of acetaminophen from disks coated with CS250-LA dispersions was significantly suppressed in fluids at pH 1.2 and 6.8, whereas disks coated with CS solution rapidly released the drug in pH 1.2 fluids. Taken together, this study shows that LA modification could be a useful approach in preparing CS films with acid stability and colonic degradability properties without requiring overcoating.

  5. PREPARATION AND EVALUATION OF HPMC-ALGINATE MUCOADHESIVE MICROCAPSULES OF DICLOFENAC FOR CONTROLLED RELEASE

    Directory of Open Access Journals (Sweden)

    K.P.R. Chowdary

    2011-11-01

    Full Text Available A new, novel promising technology for obtaining controlled release and enhancing the bioavailability is a combination of mucoadhesion principle and microencapsulation to result in mucoadhesive microcapsules. Mucoadhesive microcapsules consist of either entirely of a mucoadhesive polymer or having an outer coating enclosing the drug particles. They facilitate an intimate and prolonged contact with the absorption surface to provide controlled release and enhanced bioavailability of the contained drug over longer period of time to prolong its therapeutic action. The objective of the present work is to prepare HPMC based mucoadhesive microcapsules of diclofenac and to evaluate the microcapsules for mucoadhesiveness and controlled drug release characteristics. Spherical HPMC-alginate mucoadhesive micro- capsules of diclofenac could be prepared by the orifice – ionic gelation method. Microencapsulation efficiency was in the range 98.7 % - 103.5 %. Drug release from the HPMC – alginate microcapsules was slow and spread over a period of 12 h and depended on core: coat ratio and wall thickness of the microcapsules. Drug release mechanism from these microcapsules was by non- Fickian diffusion. Good linear relationships were observed between wall thickness of the microcapsules and release rate [K0 and K1] of the microcapsules. Mucoadhesion testing by in vitro wash-off test indicated good mucoadhesive property of HPMC-alginate microcapsules with a slower wash-off when compared to non-mucoadhesive EVA microcapsules. Thus controlled release mucoadhesive microcapsules of diclofenac could be designed employing HPMC-alginate. HPMC-alginate microcapsules of diclofenac exhibited good mucoadhesion and controlled release characteristics and were found suitable for oral controlled release of diclofenac.

  6. Self-assembled lecithin/chitosan nanoparticles for oral insulin delivery: preparation and functional evaluation.

    Science.gov (United States)

    Liu, Liyao; Zhou, Cuiping; Xia, Xuejun; Liu, Yuling

    2016-01-01

    Here, we investigated the formation and functional properties of self-assembled lecithin/chitosan nanoparticles (L/C NPs) loaded with insulin following insulin-phospholipid complex preparation, with the aim of developing a method for oral insulin delivery. Using a modified solvent-injection method, insulin-loaded L/C NPs were obtained by combining insulin-phospholipid complexes with L/C NPs. The nanoparticle size distribution was determined by dynamic light scattering, and morphologies were analyzed by cryogenic transmission electron microscopy. Fourier transform infrared spectroscopy analysis was used to disclose the molecular mechanism of prepared insulin-loaded L/C NPs. Fast ultrafiltration and a reversed-phase high-performance liquid chromatography assay were used to separate free insulin from insulin entrapped in the L/C NPs, as well as to measure the insulin-entrapment and drug-loading efficiencies. The in vitro release profile was obtained, and in vivo hypoglycemic effects were evaluated in streptozotocin-induced diabetic rats. Our results indicated that insulin-containing L/C NPs had a mean size of 180 nm, an insulin-entrapment efficiency of 94%, and an insulin-loading efficiency of 4.5%. Cryogenic transmission electron microscopy observations of insulin-loaded L/C NPs revealed multilamellar structures with a hollow core, encircled by several bilayers. In vitro analysis revealed that insulin release from L/C NPs depended on the L/C ratio. Insulin-loaded L/C NPs orally administered to streptozotocin-induced diabetic rats exerted a significant hypoglycemic effect. The relative pharmacological bioavailability following oral administration of L/C NPs was 6.01%. With the aid of phospholipid-complexation techniques, some hydrophilic peptides, such as insulin, can be successfully entrapped into L/C NPs, which could improve oral bioavailability, time-dependent release, and therapeutic activity.

  7. Preparation and evaluation of poly(caprolactone fumarate nanoparticles containing doxorubicin HCl

    Directory of Open Access Journals (Sweden)

    N Shokri

    2011-03-01

    Full Text Available Background and the purpose of the study: Biodegradable Poly(caprolactone fumarate (PCLF has been used as bioresorbable sutures. In this study, doxorubicin HCl (Dox loaded PCLF nanoparticles were prepared and characterized.  Materials and methods: PCLFs were synthesized by polycondensation of PCL diols (Mws of 530, 1250 and 2000 with fumaryl chloride. The degradation of PCLF in NaOH, water and phosphate buffer saline (PBS, was determined in terms of Mw. Nanoparticles (NPs were prepared by two methods. In microemulsion polymerization method, dichloromethane containing PCLF and photoinitiator were combined with the water containing surfactants and then placed under light for crosslinking. In nanoprecipitation method, the organic solvent containing PCLF was poured into the stirring water. The effect of several variables concentration of PCLF, poly vinyl alcohol (PVA, Dox and Trypan blue (Trb and the Mw of PCLF and PVA on NP size and loading were evaluated. Results: PCLF 530, 1250 and 2000 in PBS or water were not degrade over 28 days. Nanoprecipitaion method gave spherical (revealed by SEM images stable NPs of about 225 with narrow size distribution and a zeta potential of -43 mV. The size of NP increased significantly with increase in Mw or concentration of PCLF. Although PVA was not necessary for formation of NPs but decreased the NP size. Dox loading and EE were 2.5-6.8% and 15-20%, respectively. Increasing the drug concentration, increased the drug loading (DL and NP size. The entrapment efficiency (EE for Trb ranged from 1% for PCLF530 to 6% for PCLF2000. An increase in PCLF concentration resulted in an increase in EE. Dox and Trb release showed a burst followed by 80% and 78% release during 3 and 4 days respectively.                                                                                                           

  8. Preparation and evaluation of poly (caprolactone fumarate) nanoparticles containing doxorubicin HCI

    Science.gov (United States)

    Shokri, N.; Akbari Javar, H.; Fouladdel, Sh.; Khalaj, A.; Khoshayand, MR.; Dinarvand, R.; Atyabi, F.; Nomani, A.; Azizi, E.

    2011-01-01

    Background and the purpose of the study Biodegradable Poly(caprolactone fumarate) (PCLF) has been used as bioresorbable sutures. In this study, doxorubicin HCl (Dox) loaded PCLF nanoparticles were prepared and characterized. Material and methods PCLFs were synthesized by polycondensation of PCL diols (Mws of 530, 1250 and 2000) with fumaryl chloride. The degradation of PCLF in NaOH, water and phosphate buffer saline (PBS), was determined in terms of changes in Mw. Nanoparticles (NPs) were prepared by two methods. In microemulsion polymerization method, dichloromethane containing PCLF and photoinitiator were combined with the water containing surfactants and then the mixture was placed under light for crosslinking. In nanoprecipitation method, the organic solvent containing PCLF was poured into the stirring water. The effect of several variables including concentration of PCLF, polyvinyl alcohol (PVA), Dox and Trypan blue (Trb) and the Mw of PCLF and PVA on NP size and loading were evaluated. Result PCLF 530, 1250 and 2000 in PBS or water were not degraded over 28 days. Nanoprecipitaion method gave spherical (revealed by SEM images) stable NPs of about 225 with narrow size distribution and a zeta potential of −43 mV. The size of NP increased significantly by increase in Mw or concentration of PCLF. Although PVA was not necessary for formation of NPs, but it decreased with NP size. Dox loading and EE were 2.5–6.8% and 15–20%, respectively. Increasing the drug concentration increased the drug loading (DL) and NP size. The entrapment efficiency (EE) for Trb ranged from 1% for PCLF530 to 6% for PCLF2000. An increase in PCLF concentration resulted in an increase in EE. Dox and Trb release showed a burst followed by 80% and 78% release during 3 and 4 days respectively. Conclusion PCLF possessed suitable characteristics for preparation of nanoparticulate drug delivery system such as desired NP size, stability and degradation time. Although PCLF530 NPs were the

  9. What is organic certification?

    Science.gov (United States)

    Certified organic crop production is a holistic approach to sustainable and healthy food production to enhance the well being of the consumer, while protecting natural resources. Organic certification was implemented by the National Organic Program (NOP) in 2002 in recognition of the necessity for c...

  10. Towards FAA Certification of UAVs

    Science.gov (United States)

    Nelson, Stacy

    2003-01-01

    As of June 30, 2003, all Unmanned Aerial Vehicles (UAV), no matter how small, must adhere to the same FAA regulations as human-piloted aircraft. These regulations include certification for flying in controlled airspace and certification of flight software based on RTCA DO-178B. This paper provides an overview of the steps necessary to obtain certification, as well as a discussion about the challenges UAV's face when trying to meet these requirements. It is divided into two parts: 1) Certifications for Flying in Controlled Airspace; 2) Certification of Flight Software per RTCA DO-178B.

  11. An Analysis of State Music Education Certification and Licensure Practices in the United States

    Science.gov (United States)

    May, Brittany Nixon; Willie, Karen; Worthen, Cherilyn; Pehrson, Allyssa

    2017-01-01

    Teacher certification and licensure practices for music educators vary by state. Enhancing music teacher educator knowledge of state music education certification and licensure practices can inform music teacher preparation and improve future music teacher quality. The purpose of this study was to compile relevant information for music educators…

  12. An Analysis of the Effectiveness of Business Education Certification Programs in Texas

    Science.gov (United States)

    Lewis, Sue Evelyn Joiner

    2014-01-01

    The primary purpose of this study was to determine the effectiveness of preparation delivery programs available to those who seek business education teacher certification in Texas. There are three types of delivery programs in business education in Texas: (a) "traditional" certification programs delivered by institutions of higher…

  13. Zertifikat Niederlaendisch: Examen en getuigschrift Nederlands als vreemde taal (Certificate in Dutch)

    Science.gov (United States)

    Beersmans, Frans; Sudhoelter, Juergen

    1976-01-01

    An interim report on preparations for designing an examination in Dutch. The "unit-credit system" proposed by the Council of Europe is being used. The Dutch certificate will be comparable to the certificates given by the British Council and by the People's Universities. Sample portions are given. (Text is in German.) (IFS/WGA)

  14. Preparation and evaluation of cosmetic patches containing lactic and glycolic acids

    Directory of Open Access Journals (Sweden)

    Mahdavi H

    2006-01-01

    Full Text Available Background: Alpha-hydroxy acids such as glycolic acid (GA and lactic acid (LA, are used in cosmetic patches. The important fact in cosmetic patches is its suitable adhesion and peel properties. Aim: The objective of this study was to prepare LA- and GA-containing cosmetic patches and evaluate in-vitro/in-vivo correlation of adhesion properties. Methods: Pressure-sensitive adhesives with different concentrations of GA and LA were cast on a polyethylene terephthalate film. The patches were evaluated for peel adhesive strength. On the basis of in vitro adhesion properties the patches were selected for wear performance tests and skin irritation potential. Results: The adhesion properties (adhesion to steel plate and skin and cohesive strength tests indicated the substantial influence of GA and LA concentrations. Based on in vitro adhesion studies the patches containing 3% (w/w GA were selected for in vivo studies. In vivo studies show that a formulation containing 3% GA displays good adhesion on the skin, but it leaves little residues on the skin. Skin Irritation studies on healthy human volunteers showed negligible erythema at the site of application after 48h. Conclusion: The noninvasive patch test model was found useful for detecting irritant skin reactions to the cosmetic patch containing GA. Our results demonstrated a strong correlation between the adhesion to steel plate and adhesion to skin. But a weak correlation between the degree of adhesive residue on the skin in in vitro and in vivo tests was observed for the formulation containing 3% (w/w GA.

  15. Evaluation of a virtual reality simulation system for porcelain fused to metal crown preparation at Tokyo Medical and Dental University.

    Science.gov (United States)

    Kikuchi, Hirono; Ikeda, Masaomi; Araki, Koji

    2013-06-01

    The use of virtual reality simulation (VRS) is a new teaching modality in dentistry, and there is scope for further research evaluating its use under different educational programs. The purpose of this study was to evaluate how VRS with or without instructor feedback influenced students' learning and skills related to porcelain fused to metal (PFM) crown preparation. In this study, forty-three dental students in their fifth year of study at Tokyo Medical and Dental University, Tokyo, Japan, were randomly divided into three groups: the first group used VRS (DentSim) with the instructor's feedback (DSF) (n=15), the second group used VRS without the instructor's feedback (DS) (n=15), and the third group neither used features of VRS (NDS) (n=13) nor received the instructor's feedback. All the students performed PFM crown preparation under the same setup once a week for four weeks. Total scores, preparation time, and twelve evaluation items were compared among the three groups and four experiments. The total scores of students in the DSF and DS groups were significantly higher than those in the NDS group. The presence of the instructor did not result in significant difference when VRS was used for training, while it shortened the preparation time at early stages. The results of this study suggested that the use of the VRS system improved student training for PFM crown preparation.

  16. Pirfenidone-loaded liposomes for lung targeting: preparation and in vitro/in vivo evaluation

    Directory of Open Access Journals (Sweden)

    Meng H

    2015-06-01

    Full Text Available Hui Meng, Yong XuDepartment of Pharmaceuticals, 85th People’s Liberation Army Hospital, Shanghai, People’s Republic of China Background: The purpose of this study was to develop novel pirfenidone (PFD-loaded liposomes for targeting to the lung.Methods: The liposomes were prepared by the film hydration method, and their in vitro/vivo characteristics were evaluated.Results: The PFD liposomes appeared visually as green to yellowish suspensions and were spherical in shape. The particle size was 582.3±21.6 nm and the entrapment efficiency was relatively high (87.2%±5.7%. The liposomes showed typical sustained and prolonged drug-release behavior in vitro and fitted well with the Weibull distribution equation. The relatively slower time taken to reach a minimal plasma PFD concentration in vivo suggests that PFD liposomes have a sustained-release profile, which is consistent with the results of the in vitro release study. The PFD liposomes showed the largest area under the curve for the lung. The high distribution of PFD achieved in the lungs using this liposomal formulation may be explained by physical entrapment of the liposomes in the vascular network of the lung. Histopathological results indicated that liposomal PFD could alleviate pathological injury in lung tissue.Conclusion: This liposomal formulation can enable sustained release of PFD and increase targeting to the lung. Keywords: pirfenidone, liposomes, lung targeting, in vivo, histopathological 

  17. The preparation and evaluation of water-soluble SKLB610 nanosuspensions with improved bioavailability.

    Science.gov (United States)

    Huang, Yunchuang; Luo, Xun; You, Xinyu; Xia, Yong; Song, Xuejiao; Yu, Luoting

    2013-09-01

    The aim of the study was to investigate the potential of nanosuspension to enhance the bioavailability of SKLB610 (Biopharmaceutical Classification System class II drug), a bioactive anticancer compound synthesized in our labs. SKLB610 nanosuspensions were prepared using wet media milling. Physicochemical characteristics of the nanosuspensions were evaluated, including particle size and distribution, dissolution, transmission electron microscopy, atomic force microscopy, thermogravimetric analysis, and X-ray powder diffractometry. The dissolution rate of SKLB610 was greatly improved in nanosuspensions, compared to crude SKLB610. Pharmacokinetic studies in rats demonstrated that the oral bioavailability of SKLB610 in nanosuspension (89.4%) was 2.6-fold higher than in coarse suspension (34.1%). Stabilizer type, milling time, and milling speed had a significant effect on particle size of the SKLB610 nanosuspensions. Nanosuspensions effectively improved the dissolution rate and bioavailability of the water-insoluble drug SKLB610 by reducing the compound particle size to the nanoscale and employing a proper formulation.

  18. Characterization and Stability Evaluation of Thymoquinone Nanoemulsions Prepared by High-Pressure Homogenization

    Directory of Open Access Journals (Sweden)

    Zaki Tubesha

    2013-01-01

    Full Text Available Despite the pharmacological properties of thymoquinone (TQ, its administration in vivo remains problematic partly due to its poor water solubility, leading to low absorptivity and bioavailability. Hence, the objective of this study is to prepare, characterize, and evaluate the stability of TQ nanoemulsion (TQNE. Conventional emulsion from TQ (TQCE and empty nano- and conventional emulsions from Triolein (TRNE and TRCE are also produced for comparison purposes. The oil-in-water nanoemulsions of TQ and Triolein were produced by high-pressure homogenization. Emulsions were characterized physically by droplet size, polydispersity index, zeta potential, and refractive index. The changes of these parameters in TQNE samples stored for 6 months at 4 and 25°C were not statistically significant (P<0.05. In addition, the initial particle sizes of TQNE and TRNE were 119.6 and 119.5 nm, respectively. Stability studies were also performed for the period of 6 months. At the end of the experiment, the percent of remaining TQ in TQNE at 4, 25, and 40°C was 90.6, 89.1, and 87.4 % respectively. Slower degradation of TQ indicated the chemical stability of TQ in TQNE samples. These results indicated that TQNE is stable over a period of 6 months.

  19. Preparing Al-Mg Substrate for Thermal Spraying: Evaluation of Surface State After Different Pretreatments

    Science.gov (United States)

    Lukauskaitė, R.; Valiulis, A. V.; Černašėjus, O.; Škamat, J.; Rębiś, J. A.

    2016-08-01

    The article deals with the pretreatment technique for preparing the surface of aluminum alloy EN AW 5754 before thermal spray. The surface after different pretreatments, including degreasing with acetone, chemical etching with acidic and alkali solutions, grit-blasting, cathodic cleaning, and some combinations of these techniques, has been studied. The investigation of pre-treated surfaces covered the topographical study (using scanning electron microscopy, atomic force microscopy, and 3D profilometry), the chemical analysis by x-ray photoelectron spectroscopy, the evaluation of surface wettability (sessile drop method), and the assessment of surface free energy. Compared with all the techniques used in present work, the cathodic cleaning and its combination with grit-blasting provide the most preferable chemistry of the surface. Due to the absence of hydroxides at the surface and, possible, due to the diffusion of magnesium to the surface of substrate, the surface wettability and the surface free energy have been significantly improved. No direct correlation between the surface topography and the surface wettability has been established.

  20. Evaluation of sample preparation methods and optimization of nickel determination in vegetable tissues

    Directory of Open Access Journals (Sweden)

    Rodrigo Fernando dos Santos Salazar

    2011-02-01

    Full Text Available Nickel, although essential to plants, may be toxic to plants and animals. It is mainly assimilated by food ingestion. However, information about the average levels of elements (including Ni in edible vegetables from different regions is still scarce in Brazil. The objectives of this study were to: (a evaluate and optimize a method for preparation of vegetable tissue samples for Ni determination; (b optimize the analytical procedures for determination by Flame Atomic Absorption Spectrometry (FAAS and by Electrothermal Atomic Absorption (ETAAS in vegetable samples and (c determine the Ni concentration in vegetables consumed in the cities of Lorena and Taubaté in the Vale do Paraíba, State of São Paulo, Brazil. By means of the analytical technique for determination by ETAAS or FAAS, the results were validated by the test of analyte addition and recovery. The most viable method tested for quantification of this element was HClO4-HNO3 wet digestion. All samples but carrot tissue collected in Lorena contained Ni levels above the permitted by the Brazilian Ministry of Health. The most disturbing results, requiring more detailed studies, were the Ni concentrations measured in carrot samples from Taubaté, where levels were five times higher than permitted by Brazilian regulations.

  1. Preparation and evaluation of solid lipid nanoparticles based nanogel for dermal delivery of meloxicam.

    Science.gov (United States)

    Khurana, S; Bedi, P M S; Jain, N K

    2013-01-01

    The aim of the current investigation was to prepare and investigate the potential of solid lipid nanoparticles based gel (SLN-gel) for the dermal delivery of meloxicam (MLX). The meloxicam loaded SLN (MLX-SLN) gel was developed and characterized by means of photon correlation spectroscopy, rheometry, and differential scanning calorimetry to determine the physicochemical properties. The behavior of SLN gel on rat skin was evaluated in vitro using Franz diffusion cells to determine the skin permeation and penetration characteristics, in vivo on mice to determine the skin tolerance by histopathological examinations. The anti-inflammatory potential of SLN gel was assessed by carrageenan induced rat paw edema test. Biophysical studies including differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR) were undertaken to study the interaction between the SLN gel and skin. MLX-SLN gel with nanometric particle size exhibited the controlled release abilities and simultaneously the potential to transport the drug to various skin layers. SLN gel displayed viscoelastic properties with predominantly elastic behavior and exhibited plastic flow. Biophysical studies elucidated the interaction between the SLN gel and stratum corneum (SC) lipids, and proposed the lipid bilayer fluidization as the possible mechanism for the increased penetration of meloxicam into skin. The nano-gel system showed marked anti-inflammatory activity and excellent skin tolerability. It can be concluded that SLN gel may be a promising delivery system for MLX in the treatment of inflammatory disorders.

  2. Preparation and Microbiological Evaluation of Amphiphilic Kanamycin-Lipoamino Acid Ion-Pairs

    Directory of Open Access Journals (Sweden)

    Rosario Pignatello

    2014-05-01

    Full Text Available Amphiphilic ion-pairs of kanamycin (KAN were prepared by evaporation of a water-ethanol co-solution of KAN base and a lipoamino acid bearing a 12-carbon atoms alkyl side chain (LAA12, at different molar ratios. Infrared spectroscopy confirmed the structure of ion-pairs, while differential scanning calorimetry (DSC and powder X-ray diffractometry (PXRD studies supported the formation of new saline species with a different crystalline structure than the starting components. The solubility pattern shown in a range of both aqueous and organic solvents confirmed that the ion-pairs possess an amphiphilic character. The LAA12 counter-ion showed not to improve the antibacterial activity of KAN, suggesting that such chemical strategy is not able to favor the penetration of this drug inside the bacteria cells. Nevertheless, a slight improving, i.e., a one-fold dilution, was observed in E. coli. The present study can also serve as the basis for a further evaluation of LAA ion-pairing of antibiotics, as a means to improve the loading of hydrophilic drugs into lipid-based nanocarriers.

  3. Preparation and evaluation of mucoadhesive simvastatin microcapsules using orifice gelation technique

    Directory of Open Access Journals (Sweden)

    Trishna Bal

    2012-01-01

    Full Text Available Preparation and characterization of Simvastatin/ Hydroxy propyl beta cyclodextrin (HPBCD (SV/HPBCD binary systems by co-grinding technique and formulating the binary system in oral mucoadhesive microcapsules by using hydrophilic sodium alginate (SA and another plant seed mucilage dillenia (obtained from Dillenia indica, Family, Dilleniaceae using orifice gelation technique and systematically evaluating in vitro by using scanning electron microscopy (SEM, fourier transform infrared spectroscopy (FTIR, differential scanning calorimetry (DSC, and X-ray diffractometer (XRD. The microcapsules were smooth and elegant in appearance showed no visible cracks as confirmed by SEM; and extended drug release of 72.682% upto 12 hours in phosphate buffer of pH 6.8; showing particle size within the range of 371.5-457 μm, and less angle of repose, Hausner′s ratio and Carr′s consolidation index; and showed encapsulation efficiency of 63.068 ± 0.002 to 99.083 ± 0.017%. The in vitro release data of optimized batch of microcapsules were plotted in various kinetic equations to understand the mechanisms and kinetics of drug release, which followed zero order kinetics and value of "n," is calculated to be 0.505 and drug release was diffusion controlled. The in vivo antihyperlipidemic activity of formulations in mice was carried out developing hyperlipidemia in mice and then administering the optimized formulations orally, and the formulation showed promising results.

  4. Preparation and in vitro/in vivo evaluation of felodipine nanosuspension.

    Science.gov (United States)

    Sahu, Bhanu P; Das, Malay K

    2014-09-01

    The purpose of this study was to develop a nanosuspension of a poorly soluble drug felodipine by nanoprecipitation to achieve superior in vitro dissolution and high oral absorption in vivo in rats. Felodipine nanosuspensions were prepared by precipitation with ultrasonication method using polyvinyl alcohol (PVA) and hydroxy propyl methyl cellulose (HPMC) as stabilizers. The particle size of nanosuspension with PVA was 60-200 nm, while with HPMC is 300-410 nm. The in vitro dissolution and pharmacokinetics of optimized nanosuspensions were studied after oral administration in male wistar rats. The results showed significant improvement during in vitro dissolution and in vivo plasma level. Dissolution studies of lyophillised nanoparticles showed that up to 93.0 % dissolved in 2 h. In the in vivo evaluation, nanosuspension exhibited significant increase in AUC0-24, C max and decrease in t max. The findings revealed that particle size reduction can influence felodipine absorption in gastrointestinal tract and nanosuspension can enhance oral bioavailability of felodipine in rats.

  5. Evaluation of microstructure of A356 aluminum alloy casting prepared under vibratory conditions during the solidification

    Indian Academy of Sciences (India)

    SAKENDRA KUMAR; S P TEWARI

    2016-10-01

    The objective of this investigation was to evaluate the effect of vibrations (during solidification) on the metallurgical properties of A356 aluminum casting. Mechanical vibrations were applied to A356 aluminum alloy through set up. A356 melt has been subjected to mechanical vibration with the frequency range from 0 to 400 Hz with constant amplitude 5 lm. Grain refinement was obtained through mold vibration. Metallurgical properties were examined through optical microstructure, tensile fracture scanning electron microscope (SEM) and SEM image of test specimens prepared under different conditions of solidification. Results indicate that mold vibration effectively modified the microstructure of A356 casting and it has uniform and smaller grain sizewith fibrous silicon particle than nonvibrated casting. Grain refinement results increase in mechanical properties with increase in frequency of vibration of mold during the solidification. SEM micrograph of tensile fracture surface was carried out to study the influence of microstructure on fracture mode. SEM image of tensile fractured surface shows transgranular cleavage facets due to fracture of primary silicon particles. Fractures are brittle in nature so observation indicates low ductility and brittle fracture.

  6. Preparation and evaluation of PEO-coated materials for a microchannel hemodialyzer.

    Science.gov (United States)

    Heintz, Keely; Schilke, Karl F; Snider, Joshua; Lee, Woo-Kul; Truong, Mitchell; Coblyn, Matthew; Jovanovic, Goran; McGuire, Joseph

    2014-07-01

    The marked increase in surface-to-volume ratio associated with microscale devices for hemodialysis leads to problems with hemocompatibility and blood flow distribution that are more challenging to manage than those encountered at the conventional scale. In this work stable surface modifications with pendant polyethylene oxide (PEO) chains were produced on polydimethylsiloxane (PDMS), polycarbonate microchannel, and polyacrylonitrile membrane materials used in construction of microchannel hemodialyzer test articles. PEO layers were prepared by radiolytic grafting of PEO-polybutadiene-PEO (PEO-PB-PEO) triblock polymers to the material surfaces. Protein repulsion was evaluated by measurement of surface-bound enzyme activity following contact of uncoated and PEO-coated surfaces with β-galactosidase. Protein adsorption was decreased on PEO-coated polycarbonate and PDMS materials to about 20% of the level recorded on the uncoated materials. Neither the triblocks nor the irradiation process was observed to have any effect on protein interaction with the polyacrylonitrile membrane, or its permeability to urea. This approach holds promise as a means for in situ application of safe, efficacious coatings to microfluidic devices for blood processing that will ensure good hemocompatibility and blood flow distribution, with no adverse effects on mass transfer.

  7. Preparation, characterization and in vitro antiviral activity evaluation of foscarnet-chitosan nanoparticles.

    Science.gov (United States)

    Russo, E; Gaglianone, N; Baldassari, S; Parodi, B; Cafaggi, S; Zibana, C; Donalisio, M; Cagno, V; Lembo, D; Caviglioli, G

    2014-06-01

    A new nanoparticulate system for foscarnet delivery was prepared and evaluated. Nanoparticles were obtained by ionotropic gelation of chitosan induced by foscarnet itself, acting as an ionotropic agent in a manner similar to tripolyphosphate anion. A Doehlert design allowed finding the suitable experimental conditions. Nanoparticles were between 200 and 300nm in diameter (around 450nm after redispersion). Nanoparticle size increased after 5h, but no size increase was observed after 48h when nanoparticles were crosslinked with glutaraldehyde. Zeta potential values of noncrosslinked and crosslinked nanoparticles were between 20 and 25mV, while drug loading of noncrosslinked nanoparticles was about 40% w/w (55% w/w for crosslinked nanoparticles). Nanoparticle yield was around 25% w/w. Crosslinked nanoparticles showed a controlled drug release. Foscarnet released from nanoparticles maintained the antiviral activity of the free drug when tested in vitro against lung fibroblasts (HELF) cells infected with HCMV strain AD-169. Moreover, nanoparticles showed no toxicity on non-infected HELF cells. These nanoparticles may represent a delivery system that could improve the therapeutic effect of foscarnet.

  8. Preparation, characterization, and in vivo evaluation of tanshinone IIA solid dispersions with silica nanoparticles

    Science.gov (United States)

    Jiang, Yan-rong; Zhang, Zhen-hai; Liu, Qi-yuan; Hu, Shao-ying; Chen, Xiao-yun; Jia, Xiao-bin

    2013-01-01

    We prepared solid dispersions (SDs) of tanshinone IIA (TSIIA) with silica nanoparticles, which function as dispersing carriers, using a spray-drying method and evaluated their in vitro dissolution and in vivo performance. The extent of TSIIA dissolution in the silica nanoparticles/TSIIA system (weight ratio, 5:1) was approximately 92% higher than that of the pure drug after 60 minutes. However, increasing the content of silica nanoparticles from 5:1 to 7:1 in this system did not significantly increase the rate or extent of TSIIA dissolution. The physicochemical properties of SDs were investigated using scanning electron microscopy, differential scanning calorimetry, X-ray powder diffraction, and Fourier transforms infrared spectroscopy. Studying the stability of the SDs of TSIIA revealed that the drug content of the formulation and dissolution behavior was unchanged under the applied storage conditions. In vivo tests showed that SDs of the silica nanoparticles/TSIIA had a significantly larger area under the concentration-time curve, which was 1.27 times more than that of TSIIA (P plasma concentration and the time to reach maximum plasma concentration of the SDs were higher than those of TSIIA and the physical mixing system. Based on these results, we conclude that the silica nanoparticle based SDs achieved complete dissolution, increased absorption rate, maintained drug stability, and showed improved oral bioavailability compared to TSIIA alone. PMID:23836971

  9. Haloperidol imprinted polymer: preparation, evaluation, and application for drug assay in brain tissue.

    Science.gov (United States)

    Rahmani, Aboubakr; Mohammadpour, Amir Hooshang; Sahebnasagh, Adeleh; Mohajeri, Seyed Ahmad

    2014-11-01

    Several molecularly imprinted polymers (MIPs) were prepared in the present work, and their binding properties were evaluated in comparison with a nonimprinted polymer (NIP). An optimized MIP was selected and applied for selective extraction and analysis of haloperidol in rabbit brain tissue. A molecularly imprinted solid-phase extraction (MISPE) method was developed for cleanup and preconcentration of haloperidol in brain samples before HPLC-UV analysis. Selectivity of the MISPE procedure was investigated using haloperidol and some structurally different drugs with similar polarity that could exist simultaneously in brain tissue. The extraction and analytical process was calibrated in the range of 0.05-10 ppm. The recovery of haloperidol in this MISPE process was calculated between 79.9 and 90.4%. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.008 and 0.05 ppm, respectively. Intraday precision and interday precision values for haloperidol analysis were less than 5.86 and 7.63%, respectively. The MISPE method could effectively extract and concentrate haloperidol from brain tissue in the presence of clozapine and imipramine. Finally, the imprinted polymer was successfully applied for the determination of haloperidol in a real rabbit brain sample after administration of a toxic dose. Therefore, the proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of haloperidol in rabbit brain tissue.

  10. Electrochemical Evaluation of Thin-Film Li-Si Anodes Prepared by Plasma Spraying

    Energy Technology Data Exchange (ETDEWEB)

    GUIDOTTI,RONALD A.; REINHARDT,FREDERICK W.; SCHARRER,GREGORY L.

    1999-09-08

    Thin-film electrodes of a plasma-sprayed Li-Si alloy were evaluated for use as anodes in high-temperature thermally activated (thermal) batteries. These anodes were prepared using 44% Li/56% Si (w/w) material as feed material in a special plasma-spray apparatus under helium or hydrogen, to protect this air- and moisture-sensitive material during deposition. Anodes were tested in single cells using conventional pressed-powder separators and lithiated pyrite cathodes at temperatures of 400 to 550 C at several different current densities. A limited number of 5-cell battery tests were also conducted. The data for the plasma-sprayed anodes was compared to that for conventional pressed-powder anodes. The performance of the plasma-sprayed anodes was inferior to that of conventional pressed-powder anodes, in that the cell emfs were lower (due to the lack of formation of the desired alloy phases) and the small porosity of these materials severely limited their rate capability. Consequently, plasma-sprayed Li-Si anodes would not be practical for use in thermal batteries.

  11. Preparation and in vivo evaluation of lecithin-based microparticles for topical delivery of minoxidil.

    Science.gov (United States)

    Lee, Hyo-Jung; Oh, Dong-Won; Na, Min-Ju; Kim, Dong-Wook; Yuk, Dong-Yeon; Choi, Hyoung-Chul; Lee, Yong-Beom; Han, Kun; Park, Chun-Woong

    2017-08-02

    Minoxidil is widely used for treatment of androgenic alopecia. Commercial products containing minoxidil are usually in solution form. Repeated applications of minoxidil solution can lead to adverse effects such as skin irritation and horniness. The aims of this study were to prepare lecithin-based microparticle in minoxidil solution for enhancement of minoxidil topical delivery and skin protection and evaluate the ability of lecithin on in vitro delivery, in vivo hair growth, and skin trouble improvement compared to commercial minoxidil solution. In in vitro skin permeation study, minoxidil solution containing lecithin microparticle showed higher skin penetration rate and higher retention of drug inside the skin compared to minoxidil solution without lecithin. After topical application of minoxidil solutions with or without lecithin to C57BL/6 mice, minoxidil 5% solution containing lecithin microparticle showed hair re-growth as efficient as commercial product of minoxidil 5% solution. It also significantly improved skin troubles while commercial product presented horny substance and crust formation. Therefore, the lecithin-based microparticle in minoxidil 5% solution has good ability to promote hair growth without adverse effects.

  12. Preparation and immunogenicity-evaluation of typhoid O-specific polysaccharides bio-conjugate vaccines.

    Science.gov (United States)

    Zhehui, Peng; Chao, Pan; Peng, Sun; Erling, Feng; Jun, Wu; Li, Zhu; Qingzhong, Peng; Hengliang, Wang

    2015-05-01

    Typhoid fever caused by Salmonella Typhi is still a major public health problem in developing countries. In this study, we constructed a genetically modified Salmonella Typhi strain expressing O-specific polysaccharides (OPS) antigen conjugated to a carrier, recombinant Pseudomonas aeruginosa exotoxin A(rEPA N29). The conjugates (OPS-rEPA N29) were further purified and evaluated for their immunogenicity. The results of ELISA showed that the conjugates evoked higher titers of IgG than OPS, suggesting that rEPAN29 increased immunogenicity of OPS significantly as a carrier. Moreover, three injections with 3-week interval evoked slightly higher titers of IgG than three injections with 2-week interval. However, injection of excess conjugates could not evoke higher titers of IgG against lipid polysaccharide (LPS). In summary, our study provides a new strategy for preparing polysaccharides-protein conjugate vaccines as well as similar bio-conjugate vaccines of other Gram-negative pathogens.

  13. PREPARATION, CHARACTERIZATION AND IN VITRO EVALUATION OF REPAGLINIDE BINARY SOLID DISPERSIONS WITH HYDROPHILIC POLYMERS

    Directory of Open Access Journals (Sweden)

    Patel Manvi

    2010-09-01

    Full Text Available In the present study, the practically insoluble drug, repaglinide, employs formation of solid dispersions as a means to enhance the dissolution rate, thus enhancing bioavailability of repaglinide, typically employs hydrophilic polymer systems (Lutrol F127, PEG 6000 and Gelucire 44/14 with different ratios prepared using the melting, solvent and melting solvent methods. The formulations were evaluated for various in vitro parameters (Drug content, Drug release, FTIR, DSC, and XRD. Phase-solubility studies revealed AL type of curves for each carrier, indicating linear increase in drug solubility with carrier concentration. Good uniformity of drug content was observed with all formulations and ranged from 95.52 and 99.0%. All the solid dispersions showed dissolution improvement compare to pure drug. Solid state characterization of the drug?polymer binary systems using XRD, DSC and FTIR techniques revealed distinct loss of drug crystallinity in the formulation, ostensibly accounting for enhancement in dissolution rate. The stability studies indicated, the best formulation LMS17 was stable for period of 6 months. The solid dispersion techniques provide a promising way to increase the solubility and dissolution rate of poorly soluble drugs.

  14. Preparation, blood coagulation and cell compatibility evaluation of chitosan-graft-polylactide copolymers.

    Science.gov (United States)

    Wang, Qi; Liu, Pei; Liu, Peifeng; Gong, Tao; Li, Suming; Duan, Yourong; Zhang, Zhirong

    2014-02-01

    Biodegradable chitosan-graft-polylactide (PLA-CS) copolymers were prepared by the grafting of a poly(L-lactide) (PLLA) or poly(D-lactide) (PDLA) precursor to the backbone of chitosan using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC ⋅ HCl) and N-hydroxysuccinimide (NHS) as a coupling agent. The blood and cell compatibility of the graft copolymers were investigated in comparison to PLLA and PDLA homopolymers. The coagulation properties of PLA-CS were evaluated by hemolysis, plasma recalcification, dynamic blood clotting and protein absorption assays. PLA-CS copolymers present similar hemolysis ratio and plasma recalcification time as PLA, but slower dynamic blood clotting and lower protein absorption. The cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), agar diffusion and lactate dehydrogenase (LDH) experiments. All the samples presented no effect on the viability to cells. Inflammatory cytokine analysis using sandwich ELISAs revealed that PLA-CS would not stimulate inflammatory activity.

  15. Preparation and evaluation of sulfided NiMo/γ-Al2O3 hydrotreating catalysts

    OpenAIRE

    KAMYAB, ALI

    2016-01-01

      Four nickel-molybdenum catalysts were synthesized on gamma alumina with higher surface area and on NiMo catalyst was prepared using gamma alumina with lower surface area. Catalysts with higher-surface-area support were prepared by co-impregnation, sequential impregnation and adding phosphorous. Theses catalysts were calcined at 500  ͦC. Effect of higher calcination temperature was investigated by preparation of one catalyst calcined at 700 ͦC. Catalysts were thoroughly characterized via fou...

  16. Evaluation of phototoxic and photogenotoxic risk associated with the use of photosensitizers in suntan preparations: application to tanning preparations containing bergamot oil.

    Science.gov (United States)

    Moysan, A; Morlière, P; Averbeck, D; Dubertret, L

    1993-01-01

    Bases for the elaboration of a standardized protocol are proposed for studying phototoxic effects of skin tanning preparations containing photosensitizing agents. The experimental procedure includes in vivo phototoxicity tests, evaluation of the photogenotoxic risk and determination of the photosensitizer concentration in plasma after topical application. This procedure was carried out with tanning preparations containing a well-known photosensitizer, 5-methoxypsoralen, as a component of bergamot oil. The whole study has been performed using topical application of the commercial suntan product, i.e. containing the sunscreens and all other components. Whereas the exposure to solar simulated radiation never triggered any phototoxic response, a photosensitizing effect was observed for skin type I volunteers exposed to high doses of ultraviolet A. The transepidermal penetration resulted in a 5-methoxypsoralen concentration of 1-4 ng/ml in the suction blister fluid. The photogenotoxicity of this suction blister fluid containing 5-methoxypsoralen and also other ingredients of the tanning preparation was assayed on yeast cells and was found to be rather low. 5-Methoxypsoralen was also detected in plasma after repeated applications but at low concentrations (about 1 ng/ml) which do not present a potential risk for systemic ocular effects.

  17. CT colonography with minimal bowel preparation: evaluation of tagging quality, patient acceptance and diagnostic accuracy in two iodine-based preparation schemes

    Energy Technology Data Exchange (ETDEWEB)

    Liedenbaum, Marjolein H. [Academic Medical Centre, Department of Radiology, Amsterdam (Netherlands); Academic Medical Center, Amsterdam (Netherlands); Vries, A.H. de; Bipat, S.; Stoker, J. [Academic Medical Centre, Department of Radiology, Amsterdam (Netherlands); Gouw, C.I.B.F. [Academic Medical Centre, Department of Radiology, Amsterdam (Netherlands); Gelre Hospitals, Department of Radiology, Apeldoorn (Netherlands); Rijn, A.F. van; Dekker, E. [Academic Medical Centre, Department of Gastroenterology and Hepatology, Amsterdam (Netherlands)

    2010-02-15

    The aim of this study was to compare a 1-day with a 2-day iodine bowel preparation for CT colonography in a positive faecal occult blood test (FOBT) screening population. One hundred consecutive patients underwent CT colonography and colonoscopy with segmental unblinding. The first 50 patients (group 1) ingested 7*50 ml iodinated contrast starting 2 days before CT colonography. The latter 50 patients (group 2) ingested 4*50 ml iodinated contrast starting 1 day before CT colonography. Per colonic segment measurements of residual stool attenuation and homogeneity were performed, and a subjective evaluation of tagging quality (grade 1-5) was done. Independently, two reviewers performed polyp and carcinoma detection. The tagging density was 638 and 618 HU (p = 0.458) and homogeneity 91 and 86 HU for groups 1 and 2, respectively (p = 0.145). The tagging quality was graded 5 (excellent) in 90% of all segments in group 1 and 91% in group 2 (p = 0.749). Mean per-polyp sensitivity for lesions {>=}10 mm was 86% in group 1 and 97% in group 2 (p = 0.355). Patient burden from diarrhoea significantly decreased for patients in group 2. One-day preparation with meglumine ioxithalamate results in an improved patient acceptability compared with 2-day preparation and has a comparable, excellent image quality and good diagnostic performance. (orig.)

  18. Self-assembled lecithin/chitosan nanoparticles for oral insulin delivery: preparation and functional evaluation

    Directory of Open Access Journals (Sweden)

    Liu LY

    2016-02-01

    Full Text Available Liyao Liu, Cuiping Zhou, Xuejun Xia, Yuling Liu State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Beijing Key Laboratory of Drug Delivery Technology and Novel Formulations, Department of Pharmaceutics, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People’s Republic of China Purpose: Here, we investigated the formation and functional properties of self-assembled lecithin/chitosan nanoparticles (L/C NPs loaded with insulin following insulin–phospholipid complex preparation, with the aim of developing a method for oral insulin delivery.Methods: Using a modified solvent-injection method, insulin-loaded L/C NPs were obtained by combining insulin–phospholipid complexes with L/C NPs. The nanoparticle size distribution was determined by dynamic light scattering, and morphologies were analyzed by cryogenic transmission electron microscopy. Fourier transform infrared spectroscopy analysis was used to disclose the molecular mechanism of prepared insulin-loaded L/C NPs. Fast ultrafiltration and a reversed-phase high-performance liquid chromatography assay were used to separate free insulin from insulin entrapped in the L/C NPs, as well as to measure the insulin-entrapment and drug-loading efficiencies. The in vitro release profile was obtained, and in vivo hypoglycemic effects were evaluated in streptozotocin-induced diabetic rats.Results: Our results indicated that insulin-containing L/C NPs had a mean size of 180 nm, an insulin-entrapment efficiency of 94%, and an insulin-loading efficiency of 4.5%. Cryogenic transmission electron microscopy observations of insulin-loaded L/C NPs revealed multilamellar structures with a hollow core, encircled by several bilayers. In vitro analysis revealed that insulin release from L/C NPs depended on the L/C ratio. Insulin-loaded L/C NPs orally administered to streptozotocin-induced diabetic rats exerted a significant

  19. Preparation and evaluation of swelling induced-orally disintegrating tablets by microwave irradiation.

    Science.gov (United States)

    Sano, Syusuke; Iwao, Yasunori; Kimura, Susumu; Itai, Shigeru

    2011-09-15

    A major challenge in the development of orally disintegrating tablets (ODTs) is to achieve a good balance between tablet hardness and disintegration time. In this study, an advanced method was demonstrated to improve these opposing properties in a molded tablet using a one-step procedure that exploits the swelling induced by microwave treatment. Wet molded tablets consisting of the delta form of mannitol and silicon dioxide were prepared and microwave-heated to generate water vapor inside the tablets. This induced either swelling or shrinking of tablets, in the extent of each being dependent on tablet formulation and manufacturing conditions. A two-level full factorial design method was used to evaluate the effects of several variables in formulation and manufacturing conditions on the tablet properties, hardness, disintegration time and change in shape. The variables investigated in this study were: ratio of silicon dioxide in formulation, water volume added in granulation, ratio of water absorbed by silicon dioxide prior to granulation, and microwave irradiation time. Swelling of tablet by microwave irradiation was observed in the batches with high ratio of silicon dioxide and low levels of water volume. The disintegration time was clearly shortened by induction of the swelling, while tablet hardness increased. We demonstrated that the water vapor generated by microwave irradiation promoted a change in the crystalline form of mannitol from delta to beta, and that this may have contributed to an increase in tablet hardness. Additionally, it was found that new solid bridges were formed between the granules in the tablet via the pathway from dissolution of mannitol in water vapor to congelation, resulting in an increase in tablet hardness. Thus, both tablet hardness and disintegration properties of the molded tablets were improved by the proposed one-step method and the appropriate ranges for variables are indicated. In addition, multiple regression modeling was

  20. Preparation and Evaluation of Jojoba Oil Methyl Ester as Biodiesel and as Blend Components in Ultra Low Sulfur Diesel Fuel

    Science.gov (United States)

    The jojoba plant (Simmondsia chinensis L.) produces seeds that contain around 50 to 60 weight percent of inedible long-chain wax esters that are suitable as a potential feedstock for biodiesel production. A Jojoba oil methyl ester (JME) was prepared in effort to evaluate an important fuel propertie...

  1. The effect of apical preparation size on irrigant flow in root canals evaluated using an unsteady Computational Fluid Dynamics model

    NARCIS (Netherlands)

    Boutsioukis, C.; Gogos, C.; Verhaagen, B.; Versluis, M.; Kastrinakis, E.; van der Sluis, L.W.M.

    2010-01-01

    Aim  To evaluate the effect of apical preparation size on irrigant flow inside a root canal during final irrigation with a syringe and two different needles types, using a Computational Fluid Dynamics (CFD) model. Methodology  A validated CFD model was used to simulate the irrigant flow from either

  2. Direct certification of a class of quantum simulations

    Science.gov (United States)

    Hangleiter, D.; Kliesch, M.; Schwarz, M.; Eisert, J.

    2017-03-01

    One of the main challenges in the field of quantum simulation and computation is to identify ways to certify the correct functioning of a device when a classical efficient simulation is not available. Important cases are situations in which one cannot classically calculate local expectation values of state preparations efficiently. In this work, we develop weak-membership formulations of the certification of ground state preparations. We provide a non-interactive protocol for certifying ground states of frustration-free Hamiltonians based on simple energy measurements of local Hamiltonian terms. This certification protocol can be applied to classically intractable analog quantum simulations: For example, using Feynman-Kitaev Hamiltonians, one can encode universal quantum computation in such ground states. Moreover, our certification protocol is applicable to ground state encodings of IQP circuits aiming at the demonstration of quantum supremacy. These can be certified efficiently when the error is polynomially bounded.

  3. Evaluating the impact of a pre-rotation workshop on student preparation for clinical advanced pharmacy practice experiences

    Directory of Open Access Journals (Sweden)

    Medina MS

    2008-12-01

    Full Text Available Objectives: This pilot study was designed to evaluate the impact of a pre-rotation workshop (PRW on pharmacy students’ clinical skills and preparation for clinical Advanced Pharmacy Practice Experiences (APPE involving direct patient care. Methods: Randomized controlled trial of an educational intervention with Institutional Review Board approval. PRW activities designed to simulate rotation activities around five competencies, patient charts, medication histories, SOAP notes, patient presentations, and professionalism. Endpoints were evaluated using clinical rotation preceptors’ evaluation of performance and students’ performance on objective structured clinical exams (OSCE.Results: Eight fourth-year students and eight GPA matched controls (20% of the total class were selected to voluntarily participate. The PRW demonstrated a positive impact on students’ clinical skills and preparation for rotations by improving OSCE performance. However, no significant differences were found between groups when comparing preceptor evaluations of skills on rotations. These results are limited by the small sample size, potential OSCE “test-wiseness” effects, lack of OSCE evaluator blinding to study groups, potential case specificity effects due to the limited number of cases used on the OSCE and possible lack of sensitivity of the rotation evaluation tool to capture true differences among the experimental and control group participants.Conclusion: The PRW was successful at advancing students’ clinical skills and preparation for rotations and may be considered as a tool to help bridge didactic to clinical experiences in the Pharm.D. curriculum.

  4. Certification/enforcement analysis

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-06-01

    Industry compliance with minimum energy efficiency standards will be assured through a two-part program approach of certification and enforcement activities. The technical support document (TSD) presents the analyses upon which the proposed rule for assuring that consumer product comply with applicable energy efficiency standards is based. Much of the TSD is based upon support provided DOE by Vitro Laboratories. The OAO Corporation provided additional support in the development of the sampling plan incorporated in the proposed rule. Vitro's recommended approach to appliance certification and enforcement, developed after consideration of various program options, benefits, and impacts, establishes the C/E program framework, general criteria, and procedures for assuring a specified level of energy efficiency performance of covered consumer products. The results of the OAO analysis are given in Volume II of the TSD.

  5. Certification/enforcement analysis

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-06-01

    Industry compliance with minimum energy efficiency standards will be assured through a two-part program approach of certification and enforcement activities. The technical support document (TSD) presents the analyses upon which the proposed rule for assuring that consumer product comply with applicable energy efficiency standards is based. Much of the TSD is based upon support provided DOE by Vitro Laboratories. The OAO Corporation provided additional support in the development of the sampling plan incorporated in the proposed rule. Vitro's recommended approach to appliance certification and enforcement, developed after consideration of various program options, benefits, and impacts, establishes the C/E program framework, general criteria, and procedures for assuring a specified level of energy efficiency performance of covered consumer products. The results of the OAO analysis are given in Volume II of the TSD.

  6. The evaluation, certification and approbation of oil and gas reserves in Mexico; facts and performance; Sistema de estimacion, certificacion y aprobacion de reservas de hidrocarburos en Mexico; analisis de desempeno

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez-Padilla, Victor [Universidad Nacional Autonoma de Mexico (Mexico)]. E-mail: energia123@hotmail.com

    2013-07-15

    The evaluation, certification, approval and disclosure system of reserves applied in Mexico since 2010 aims to replicate the best practices of the international oil industry. It has gained rigor, simplicity, truthfulness and transparency, however its performance has been unequal: it has achieved to provide more certitude over the proved reserves but it has failed to dissipate the uncertainty over the probable and possible reserves. [Spanish] El sistema de evaluacion, certificacion y aprobacion de reservas aplicado en Mexico desde 2010 busca reproducir las mejores practicas de la industria petrolera internacional. Ha ganado en rigor, sencillez, veracidad y transparencia, sin embargo, su desempeno ha sido desigual, pues ha logrado brindar mayor certeza sobre las reservas probadas, pero no ha podido disipar la incertidumbre sobre las reservas probables y posibles.

  7. 33 CFR 159.15 - Certification.

    Science.gov (United States)

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Certification. 159.15 Section 159... MARINE SANITATION DEVICES Certification Procedures § 159.15 Certification. (a) The recognized facility... certified, the Coast Guard includes a certification number for the device. If certification is denied,...

  8. 30 CFR 850.15 - Certification.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Certification. 850.15 Section 850.15 Mineral..., EXAMINATION, AND CERTIFICATION OF BLASTERS PERMANENT REGULATORY PROGRAM REQUIREMENTS-STANDARDS FOR CERTIFICATION OF BLASTERS § 850.15 Certification. (a) Issuance of certification. The regulatory authority...

  9. 9 CFR 151.4 - Pedigree certificate.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Pedigree certificate. 151.4 Section... Purebred Animals § 151.4 Pedigree certificate. A pedigree certificate for an animal of a breed listed in... English in the pedigree certificate for the animal or in a separate certificate appended to the pedigree...

  10. Immunohistochemical evaluation of endothelial cell adhesion molecules in human dental pulp: effects of tooth preparation and adhesive application.

    Science.gov (United States)

    Bagis, Bora; Atilla, Pergin; Cakar, Nur; Hasanreisoglu, Ufuk

    2007-08-01

    Studies have demonstrated that restorative procedures can initiate pulpal inflammation. Adhesion molecules on endothelial cells mediate the leukocyte-endothelium interaction, which is the fundamental event of inflammation. The aim of this study was to evaluate possible changes in the endothelial cell adhesion molecules (CAMs) of human dental pulp with tooth preparation, and after the application of one-step self-etch adhesive. Twenty healthy human premolars and third molars scheduled to be extracted for orthodontic reasons were randomly assigned to four experimental groups. Group 1 involved sound intact teeth representing the controls. In group 2, teeth were prepared for full crown and extracted within 2h. Groups 3 and 4 comprised the teeth coated with one-step self-etch adhesive, iBond Gluma inside following the preparation and extracted after 24 and 48h, respectively. Tissue distribution and staining intensity of CAMs including E-selectin, P-selectin, ICAM-1, VCAM-1 and PECAM-1 was investigated in the pulp samples using monoclonal antibodies and the streptavidin-biotin-horse-radish immunoperoxidase procedure. The assessment of immunohistochemical reactions was performed by two independent observers using a semi-quantitative scale. All the CAMs evaluated were expressed by the healthy pulp tissues. Significant alterations in the distribution and staining intensity of CAMs were detected following tooth preparation. One-step self-etch adhesive tested in the present study induced inflammatory reactions in the pulp (Padhesive on prepared teeth had a potential to interfere with the inflammatory response.

  11. PREPARATION, CHARACTERIZATION AND EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF AN INTRAVENOUS LIPOSOMAL FORMULATION OF BIS-DEMETHOXY CURCUMIN ANALOGUE (BDMCA

    Directory of Open Access Journals (Sweden)

    PREPARATION, CHARACTERIZATION AND EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF AN INTRAVENOUS LIPOSOMAL FORMULATION OF BIS-DEMETHOXY CURCUMIN ANALOGUE (BDMCA

    2009-12-01

    Full Text Available The aim of study was to prepare small unilamellar vesicles (SUVs incorporating BDMCA that can injected by intravenousroute and further, evaluate hepatoprotective activity of the formulation. SUV liposomes were prepared using thin filmhydration followed by sonication method. Soya lecithin was used as lipid and stearyl amine was used as cationic chargeinducer. In the preparation of liposomes, process and formulation parameters were standardized. After preparation SUVswere characterized for physicochemical properties, particle size, zetapotential, percent drug entrapment, in vitro drugrelease and the drug-polymer interaction. The sustenance of drug release into the plasma after intravenous BDMCA SUVadministration was determined. Hepatoprotective activity was evaluated in CCl4 treated rats. The liposomal formulationswere successfully prepared using thin film hydration followed by sonication method. The desired encapsulation wasachieved by increase in the area of the lipid film formed. The size of SUVs obtained was 327 nm. FTIR results indicate therewas no interaction between lipid and drug. In vitro release data showed that the release was sustained for 10 days in vitroand could be described as diffusion-controlled. The liposomal formulations were able to sustain the release of drug in vivoalso. Liposomal formulations showed better hepatoprotective activity to the drug compared to its solution form.

  12. Formulation and stability evaluation of extemporaneously prepared atenolol capsules from crushed atenolol tablets.

    Science.gov (United States)

    Zaid, Abdel Naser; Malkieh, Numan; Kharoaf, Maher; Abu Ghoush, Abeer; Al-Ramahi, Rowa'

    2012-01-01

    The purpose of this study was to formulate a 25-mg atenolol capsule starting from a commercial 100-mg atenolol tablet, given the fact that this strength is not available in Palestine and also because 50-mg atenolol tablets failed the splitting uniformity test of the European Pharmacopoeia, and to evaluate the chemical stability and dissolution behavior of the obtained capsules so as to ensure a high-quality product. A high-performance liquid chromatographic system was used for the analysis and quantification of atenolol in the samples studied. Samples of atenoIol for analysis were prepared as reported by the United States Pharmacopeia monograph. Disintegration and dissolution tests were performed according to the United States Pharmacopeia. The high-performance liquid chromatography assay indicated that the 25-mg atenolol capsules were stable for four months when stored at ambient temperature conditions. The disintegration time for all atenolol capsules was within the United States Pharmacopeia limits of 15 minutes. Atenolol release profile showed that approximately 90% of atenolol dissolved after 10 minutes. This study is important for patients who need to take one half of a 50-mg tablet, but for whom the splitting process doesn't give equal halves, and also for modifying the dose for patients with renal or hepatic problems. Therefore, it is possible for the community pharmacist to crush atenolol 100-mg tablets and refill them in new capsules with each containing a precise amount of atenolol, calculated according to body surface area and kidney and liver functions without affecting the chemical stability of the active ingredient nor its dissolution profile and also have a cost effective dosage form.

  13. Compound antimalarial ethosomal cataplasm: preparation, evaluation, and mechanism of penetration enhancement.

    Science.gov (United States)

    Shen, Shuo; Liu, Shu-Zhi; Zhang, Yu-Shi; Du, Mao-Bo; Liang, Ai-Hua; Song, Li-Hua; Ye, Zu-Guang

    2015-01-01

    Malaria is still a serious public health problem in some parts of the world. The problems of recurrence and drug resistance are increasingly more serious. Thus, it is necessary to develop a novel antimalarial agent. The objectives of this study were to construct a novel compound antimalarial transdermal nanosystem-ethosomal cataplasm, to investigate its characteristics and efficiency, and to systematically explore the penetration-enhancing mechanisms of ethosomal cataplasm. Artesunate-loaded ethosomes and febrifugine-loaded ethosomes were prepared, and their characteristics were evaluated. Drug-loaded ethosomes were incorporated in the matrix of cataplasm to form the compound antimalarial ethosomal cataplasm. With the help of ethosomal technology, the accumulated permeation quantity of artesunate significantly increased at 8 hours after administration, which was 1.57 times as much as that of conventional cataplasm. Soon after administration, the ethosomal cataplasm could make a large quantity of antimalarial drug quickly penetrate through skin, then the remaining drug in the ethosomal cataplasm could be steadily released. These characteristics of ethosomal cataplasm are favorable for antimalarial drugs to kill Plasmodium spp. quickly and prevent the resurgence of Plasmodium spp. As expected, the ethosomal cataplasm showed good antimalarial efficiency in this experiment. The negative conversion rates were 100% and the recurrence rates were 0% at all dosages. The mechanism of penetration enhancement of the ethosomal cataplasm was systematically explored using an optics microscope, polarization microscope, and transmission electron microscopy. The microstructure, ultrastructure, and birefringent structure in skin were observed. Data obtained in this study showed that the application of ethosomal technology to antimalarial cataplasm could improve the transdermal delivery of drug, enhance the efficacy, and facilitate practical application in clinic.

  14. Enhanced oral bioavailability of docetaxel by lecithin nanoparticles: preparation, in vitro, and in vivo evaluation

    Science.gov (United States)

    Hu, Kaili; Cao, Shan; Hu, Fuqiang; Feng, Jianfang

    2012-01-01

    The aim of this research work was to investigate the potential of lecithin nanoparticles (LNs) in improving the oral bioavailability of docetaxel. Docetaxel-loaded LNs (DTX-LNs) were prepared from oil-in-water emulsions and characterized in terms of morphology, size, zeta potential, and encapsulation efficiency. The in vitro release of docetaxel from the nanoparticles was studied by using dialysis bag method. Caco-2 cell monolayer was used for the in vitro permeation study of DTX-LNs. Bioavailability studies were conducted in rats and different pharmacokinetic parameters were evaluated after oral administration of DTX-LNs. The results showed that DTX-LNs had a mean diameter of 360 ± 8 nm and exhibited spherical shape with smooth surface under transmission electron microscopy. The DTX-LNs showed a sustained-release profile, with about 80% of docetaxel released within 72 hours. The apical to basolateral transport of docetaxel across the Caco-2 cell monolayer from the DTX-LNs was 2.14 times compared to that of the docetaxel solution (0.15 × 10−5 ± 0.016 × 10−5 cm/second versus 0.07 × 10−5 ± 0.003 × 10−5 cm/second). The oral bioavailability of the DTX-LNs was 3.65 times that of docetaxel solution (8.75% versus 2.40%). These results indicate that DTX-LNs were valuable as an oral drug delivery system to enhance the absorption of docetaxel. PMID:22848177

  15. 42 CFR 493.43 - Application for registration certificate, certificate for provider-performed microscopy (PPM...

    Science.gov (United States)

    2010-10-01

    ..., certificate for provider-performed microscopy (PPM) procedures, and certificate of compliance. 493.43 Section... Provider-performed Microscopy Procedures, and Certificate of Compliance § 493.43 Application for registration certificate, certificate for provider-performed microscopy (PPM) procedures, and certificate...

  16. A Study on the Form of English Examination and Evaluation in Vocational Colleges Related to“Double Certificates Inte-gration”%基于“双证融通”的高职英语考核评价方式改革研究

    Institute of Scientific and Technical Information of China (English)

    葛丽萍; 王彦侠

    2015-01-01

    Double certificates integration system tends to be the inevitable trend of the reform of higher vocational education. In or⁃der to accomplish the integration between academic certificates and professional qualification certificates, the higher vocational col⁃leges need to improve the previous single test theory, increase the weight of the professional skills of the exam, and build an assess⁃ment system based on the"double certificate". This research, which lays stress on the evaluation methods, attempts to explore the effective channels of vocational English teaching and double certificates integration.%双证融通是高职教育人才培养模式改革的必经之路。如何引导高职英语教育真正实现学历证书与职业资格证书之间的有效融通,就需要推翻以往单一的以测试理论水平为主的考试,加大专业职业技能的考试权重,构建基于“双证融通”的考核体系。文章试图从考核评价方式入手,探寻高职英语教学双证融通的有效渠道。

  17. Preparation and Evaluation of Veterinary 0.1% Injectable Solution of Atropine Sulphate

    Directory of Open Access Journals (Sweden)

    F K Mohammad

    2012-06-01

    Full Text Available This study introduces the know-how of preparing a multiple injection form atropine sulphate solution. An injectable aqueous solution of atropine sulphate at a concentration of 0.1%. was prepared under aseptic conditions in dark glass bottles each containing 50 ml. The preparation was intended for animal use only. It contained 1g atropine sulphate, 9 g sodium chloride as a normal saline, benzyl alcohol 15 ml as a preservative and water for injection up to 1000 ml. The pH of the solution was adjusted to 4.2 (range 3.0-6.5. The preparation of 0.1% atropine sulphate solution was clear colorless solution free from undesired particles. It complied with the requirements for injectable solutions. Further, the preparation was safe when used under laboratory conditions in chicks, rats and donkeys. It was also effective in preventing dichlorvos (an organophosphate insecticide-induced poisoning in chicks in a manner comparable to a commercial preparation of 0.1% atropine sulphate. In conclusion, the know-how of a preparation of 0.1% atropine sulphate solution is presented for veterinary use. [Vet. World 2012; 5(3.000: 145-149

  18. Certification trails for data structures

    Science.gov (United States)

    Sullivan, Gregory F.; Masson, Gerald M.

    1993-01-01

    Certification trails are a recently introduced and promising approach to fault detection and fault tolerance. The applicability of the certification trail technique is significantly generalized. Previously, certification trails had to be customized to each algorithm application; trails appropriate to wide classes of algorithms were developed. These certification trails are based on common data-structure operations such as those carried out using these sets of operations such as those carried out using balanced binary trees and heaps. Any algorithms using these sets of operations can therefore employ the certification trail method to achieve software fault tolerance. To exemplify the scope of the generalization of the certification trail technique provided, constructions of trails for abstract data types such as priority queues and union-find structures are given. These trails are applicable to any data-structure implementation of the abstract data type. It is also shown that these ideals lead naturally to monitors for data-structure operations.

  19. Preparation and Evaluation of a Novel Cellulose Tris(N-3,5-dimethylphenylcarbamate) Chiral Stationary Phase

    Institute of Scientific and Technical Information of China (English)

    GE,Jin; ZHAO,Liang; SHI,Yan-Ping

    2008-01-01

    A novel cellulose tris(N-3,5-dimethylphenylcarbamate) (CDMPC) chiral stationary phase (CSP) was prepared by coating CDMPC on TiO2/SiO2, which was prepared by coating titania nanoparticles on silica through a self-assemble technique. At first, 2-hydroxyl-phenyl acetonitrile and α-phenylethanol were separated on this new CSP to evaluate the chiral separation ability. Then, two pesticides, matalaxyl and diclofop-methyl were separated.The influence of the mobile phase composition on the enantioselectivity was discussed, and the repeatability and stability of the CSP were studied too.

  20. On the Detection of Fake Certificates via Attribute Correlation

    Directory of Open Access Journals (Sweden)

    Xiaojing Gu

    2015-06-01

    Full Text Available Transport Layer Security (TLS and its predecessor, SSL, are important cryptographic protocol suites on the Internet. They both implement public key certificates and rely on a group of trusted certificate authorities (i.e., CAs for peer authentication. Unfortunately, the most recent research reveals that, if any one of the pre-trusted CAs is compromised, fake certificates can be issued to intercept the corresponding SSL/TLS connections. This security vulnerability leads to catastrophic impacts on SSL/TLS-based HTTPS, which is the underlying protocol to provide secure web services for e-commerce, e-mails, etc. To address this problem, we design an attribute dependency-based detection mechanism, called SSLight. SSLight can expose fake certificates by checking whether the certificates contain some attribute dependencies rarely occurring in legitimate samples. We conduct extensive experiments to evaluate SSLight and successfully confirm that SSLight can detect the vast majority of fake certificates issued from any trusted CAs if they are compromised. As a real-world example, we also implement SSLight as a Firefox add-on and examine its capability of exposing existent fake certificates from DigiNotar and Comodo, both of which have made a giant impact around the world.

  1. Novice Teachers' Perceptions of Preparedness to Teach by Certification Route

    Science.gov (United States)

    Wooten, Karen E.

    2009-01-01

    The purpose of this study was to examine the effects of certification route (traditional versus non-traditional) on second-year, core content area (English, mathematics, science, and social studies) high school teachers' perceptions of their preparedness to teach. The curricular model for teacher preparation proposed by Feiman-Nemser (2001) served…

  2. State Certification Requirements for Early Childhood Special Educators

    Science.gov (United States)

    Stayton, Vicki D.; Dietrich, Sylvia L.; Smith, Barbara J.; Bruder, Mary Beth; Mogro-Wilson, Cristina; Swigart, Ashley

    2009-01-01

    The Office of Special Education Programs funded Center to Inform Personnel Preparation Policy and Practice in Early Intervention and Preschool Education conducted a study to obtain current information about state certification requirements for early childhood special educators who work with preschool children with developmental delays and…

  3. Efficient Certificate Management in VANET

    CERN Document Server

    Samara, Ghassan

    2012-01-01

    Vehicular Ad hoc Networks is one of the most challenging research area in the field of Mobile Ad Hoc Networks, in this research We propose a flexible, simple, and scalable design for VANET certificates, and new methods for efficient certificate management, which will Reduce channel overhead by eliminating the use of CRL, and make Better certificate Revocation Management. Also it will increase the security of the network and helps in identifying the adversary vehicle.

  4. CFPC's Certification Examination: An overview

    OpenAIRE

    Handfield-Jones, Richard; Hollingworth, Gary R

    1990-01-01

    The certification examination of the College of Family Physicians of Canada is designed to assess the extent to which the College's educational objectives have been achieved. Since the first examination in 1969, more than 7000 physicians have received their certification. The authors describe the basic elements of this test and the process through which the Committee on Examinations designs and sets the examination. The authors comment on the role of the certification process in the education...

  5. Prospective evaluation of small bowel preparation with bisacodyl and sodium phosphate for capsule endoscopy

    Institute of Scientific and Technical Information of China (English)

    Andreas Franke; Frank Hummel; Phillip Knebel; Christoph Antoni; Ulrich Bocker; Manfred V Singer; Matthias Lohr

    2008-01-01

    AIM: To determine the effect of Prepacol?, a combination of sodium phosphate and bisacodyl, on transit and quality of capsule endoscopy (CE).METHODS: Fivety two consecutive patients were included in this prospective study. CE was performed following a 12 h fasting period. Twenty six patients were randomized for additional preparation with Prepacor. The quality of CE was assessed separately for the proximal and the distal small bowel by 3 experienced endoscopists on the basis of a graduation which was initially developed with 20 previous CE.RESULTS: Preparation with Prepacol? accelerated small bowel transit time (262 ± 55 min vs 287 ± 97 min), but had no effect on the quality of CE. Visibility was significantly reduced in the distal compared to the proximal small bowel.CONCLUSION: The significantly reduced visibility of CE in the distal small bowel allocates the need for a good preparation. Since Prepacol? has no beneficial effect on CE the modality of preparation and the ideal time of application remains unclear. Further standardized examinations are necessary to identify sufficient preparation procedures and to determine the impact of the volume of the preparation solution.

  6. Fuel Gas Demonstration Plant Program: Small-Scale Industrial Project. Certificate of need

    Energy Technology Data Exchange (ETDEWEB)

    None

    1978-12-01

    This Certificate of Need draft was prepared to meet new requirements as imposed by the Minnesota Energy Agency (MEA) since the original Erie/DOE contract was signed. The preparation of this document was authorized with the approval of the Certificate of Need contained in Contract Amendment No. A-005 of the Erie/DOE contract. With the issue of the Certificate of Need draft, Erie Mining Company considers this document requirement complete as it pertains to Phase I activities and delivered to DOE in accordance with Erie/DOE contract EW-78-C-02-5066 Appendix A, Part 3.I.F.5.

  7. COSPAS-SARSAT Beacon Certification Facility

    Data.gov (United States)

    Federal Laboratory Consortium — EPG's COSPAS-SARSAT Beacon Certification Facility is one of five certification facilities in the world. Formal certifications are available for all beacon types and...

  8. 77 FR 70176 - Previous Participation Certification

    Science.gov (United States)

    2012-11-23

    ... URBAN DEVELOPMENT Previous Participation Certification AGENCY: Office of the Chief Information Officer... digital submission of all data and certifications is available via HUD's secure Internet systems. However...: Previous Participation Certification. OMB Approval Number: 2502-0118. Form Numbers: HUD-2530 ....

  9. Forcecom Developer Certification Handbook (DEV401)

    CERN Document Server

    Kabe, Siddhesh

    2012-01-01

    A practical guide that will help you discover fundamentals for building applications on Force.com without writing a single line of code. If you are new to the Force.com platform and want to learn from scratch, it is advisable that you begin with the first chapter and move on to the last. However, if you are familiar with Force.com and want to understand in detail some aspect of it, you can dip into any chapter. Each certification module is covered in a separate chapter, and is accompanied by questions being asked in the exams. Developers who need to prepare for the Salesforce.com Certified For

  10. LPI Linux Certification in a Nutshell

    CERN Document Server

    Haeder, Adam; Pessanha, Bruno; Stanger, James

    2010-01-01

    Linux deployment continues to increase, and so does the demand for qualified and certified Linux system administrators. If you're seeking a job-based certification from the Linux Professional Institute (LPI), this updated guide will help you prepare for the technically challenging LPIC Level 1 Exams 101 and 102. The third edition of this book is a meticulously researched reference to these exams, written by trainers who work closely with LPI. You'll find an overview of each exam, a summary of the core skills you need, review questions and exercises, as well as a study guide, a practice test,

  11. Audit of a Scientific Data Center for Certification as a Trustworthy Digital Repository: A Case Study

    Science.gov (United States)

    Downs, R. R.; Chen, R. S.

    2011-12-01

    Services that preserve and enable future access to scientific data are necessary to ensure that the data that are being collected today will be available for use by future generations of scientists. Many data centers, archives, and other digital repositories are working to improve their ability to serve as long-term stewards of scientific data. Trust in sustainable data management and preservation capabilities of digital repositories can influence decisions to use these services to deposit or obtain scientific data. Building on the Open Archival Information System (OAIS) Reference Model developed by the Consultative Committee for Space Data Systems (CCSDS) and adopted by the International Organization for Standardization as ISO 14721:2003, new standards are being developed to improve long-term data management processes and documentation. The Draft Information Standard ISO/DIS 16363, "Space data and information transfer systems - Audit and certification of trustworthy digital repositories" offers the potential to evaluate digital repositories objectively in terms of their trustworthiness as long-term stewards of digital resources. In conjunction with this, the CCSDS and ISO are developing another draft standard for the auditing and certification process, ISO/DIS 16919, "Space data and information transfer systems - Requirements for bodies providing audit and certification of candidate trustworthy digital repositories". Six test audits were conducted of scientific data centers and archives in Europe and the United States to test the use of these draft standards and identify potential improvements for the standards and for the participating digital repositories. We present a case study of the test audit conducted on the NASA Socioeconomic Data and Applications Center (SEDAC) and describe the preparation, the audit process, recommendations received, and next steps to obtain certification as a trustworthy digital repository, after approval of the ISO/DIS standards.

  12. SEM evaluation of the hybrid layer after cavity preparation with Er:YAG laser.

    Science.gov (United States)

    de Barceleiro, Marcos Oliveira; Dias, Kátia Regina Hostílio Cervantes; Sales, Helena Xavier; Silva, Bárbara Carvalho; Barceleiro, Cristiane Gomes

    2008-01-01

    This study compared the thickness of the hybrid layer formed using Scotchbond Multi-Purpose Plus, Single Bond 2, Prime & Bond 2.1 and Xeno III on a dentin surface prepared with a diamond bur in a high speed handpiece or prepared with an Er:YAG laser used with two parameters of pulse energy (200 and 400 mJ) and two parameters of frequency (4 and 6 Hz). Flat dentin surfaces obtained from 20 human third molars were treated with the two methods and were then prepared with the dentin adhesive systems according to the manufacturers' instructions. After a layer of composite was applied, the specimens were sectioned, flattened, polished and prepared for Scanning Electronic Microscopy observation. Five different measurements of the hybrid layer thickness were obtained along the bonded surface in each specimen. The results were statistically analyzed using Analysis of Variance and Student-Newman-Keuls tests (p cavity preparation method, four groups were formed: Group I (diamond bur) > Group II (Laser 200 mJ/4 Hz) = Group III (Laser 200 mJ/6 Hz) > Group IV (Laser 400 mJ/4 Hz) > Group V (Laser 400 mJ/6 Hz). When comparing the dentin adhesive systems, there were no statistically significant differences. These results showed that the four tested dentin adhesive systems produced a 2.90 +/- 1.71 microm hybrid layer in dentin prepared with a diamond bur. This hybrid layer was regular and routinely found. In the laser groups, the dentin adhesive systems produced hybrid layers ranging from 0.41 +/- 1.00 microm to 2.06 +/- 2.49 microm, which were very irregular and not routinely found. It was also concluded that the Er:YAG laser, with the parameters used in this experiment, has a negative influence on the formation of a hybrid layer and cavity preparation methods influence formation of the hybrid layer.

  13. Preparation and evaluation of a biomimetic scaffold with porosity gradients in vitro

    Directory of Open Access Journals (Sweden)

    Qianbin Wang

    2012-03-01

    Full Text Available A novel biodegradable scaffold based on mimetic a natural bone tissue morphology with a porosity gradient structure was prepared in this paper. The result of surface morphology indicated that a graded porous structure was formed in the fabricated scaffold, where the dense layer (0% was connected with the most porous layer (60% by a middling porous layer (30%. To evaluate the degradability, graded porous scaffolds compared with homogeneous scaffolds were placed into a Tris-HCl buffer solution (pH = 7.4 for 28 days. It was found that both scaffolds presented the same degradation trend, and the graded porous structure did not change the original degradability of the scaffold. Moreover, the compressive strength of the graded porous scaffold was better than that of conventional homogeneous scaffold with the increase of degradation time, and the graded porous structure can enhanced the mechanical property of the scaffold. These findings suggest that this biodegradable and porosity-graded scaffold may be a new promising scaffold for loaded bone implant.Um novo esqueleto mimetizando a morfologia de tecido ósseo e com uma estrutura de porosidade gradiente foi preparado e é descrito neste artigo. O resultado da avaliação da morfologia da superfície indicou que uma estrutura porosa gradiente se formou no esqueleto fabricado no qual uma camada densa (0% foi conectada com a camada mais porosa (60% por uma camada porosa média (30%. Para avaliar a degradabilidade, esqueletos de porosidade gradiente e esqueletos homogêneos foram colocados em uma solução tampão Tris-HCL (pH = 7,4 durante 28 dias. Observou-se que ambos os esqueletos apresentaram a mesma tendência de degradação e a estrutura de porosidade gradiente não modificou a degradabilidade original do esqueleto. Além disso, a força compressiva do esqueleto de porosidade gradiente foi melhor do que aquela do esqueleto homogêneo convencional, com aumento do tempo de degradação, e que a

  14. Preparation and clinical evaluation of nano-transferosomes for treatment of erectile dysfunction

    Directory of Open Access Journals (Sweden)

    Ali MFM

    2015-04-01

    Full Text Available Maha Fadel M Ali,1 Heba F Salem,2 Hany F Abdelmohsen,3 Sameh K Attia4 1Pharmaceutical Technology Unit, Medical Applications of Lasers Department, National Institute of Laser Enhanced Science, Cairo University, Cairo, Egypt; 2Pharmaceutics and Industrial Pharmacy Department, 3Clinical Pharmacy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt; 4Dermatology and Andrology Department, Faculty of Medicine, Minia University, Minia, Egypt Objective: The goal of the present study was to formulate topical nanocarriers of the low-cost vasodilator, papaverine hydrochloride (PH, as an alternative to the painful penile injections. The injections are used for both diagnosis and treatment of erectile dysfunction. Transdermal nano-transferosome (T, the ultraflexible nanoliposome, was used as a nanocarrier to enhance the penetration of the papaverine to the penis. Methods: Different nano formulas were prepared and characterized for their encapsulation efficiency, particle size, zeta potential, and cumulative drug release. The formula acquired the best characteristics was incorporated into 2% (w/v hydroxypropyl methylcellulose hydrogel base. The gel containing transferosomal papaverine hydrochloride (PH and that containing free PH were clinically compared using color flow Doppler measurements. Results: The results revealed that transferosome 3 (T3 had the highest entrapment efficiency approaching 72%, low particle size of 220 nm, and zeta potential of -33.4 mV. The formula released 73% of its initial drug content within 2 hours. The clinical evaluation showed the increase in the cavernous artery diameter from 0.53 mm to 0.78 mm and the increase in the peak systolic flow velocity from 5.95 cm/second to 12.2 cm/second, both of which were found to be significant at P<0.05. Conclusion: It is evident from the study that the transferosomes can be used as a carrier of papaverine hydrochloride for both diagnosis and treatment of the

  15. Preparation, characterization, and in vivo evaluation of tanshinone IIA solid dispersions with silica nanoparticles

    Directory of Open Access Journals (Sweden)

    Jiang YR

    2013-06-01

    Full Text Available Yan-rong Jiang,1,2 Zhen-hai Zhang,1 Qi-yuan Liu,1,2 Shao-ying Hu,1,2 Xiao-yun Chen,1,2 Xiao-bin Jia11Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of China; 2College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, People’s Republic of ChinaAbstract: We prepared solid dispersions (SDs of tanshinone IIA (TSIIA with silica nanoparticles, which function as dispersing carriers, using a spray-drying method and evaluated their in vitro dissolution and in vivo performance. The extent of TSIIA dissolution in the silica nanoparticles/TSIIA system (weight ratio, 5:1 was approximately 92% higher than that of the pure drug after 60 minutes. However, increasing the content of silica nanoparticles from 5:1 to 7:1 in this system did not significantly increase the rate or extent of TSIIA dissolution. The physicochemical properties of SDs were investigated using scanning electron microscopy, differential scanning calorimetry, X-ray powder diffraction, and Fourier transforms infrared spectroscopy. Studying the stability of the SDs of TSIIA revealed that the drug content of the formulation and dissolution behavior was unchanged under the applied storage conditions. In vivo tests showed that SDs of the silica nanoparticles/TSIIA had a significantly larger area under the concentration-time curve, which was 1.27 times more than that of TSIIA (P < 0.01. Additionally, the values of maximum plasma concentration and the time to reach maximum plasma concentration of the SDs were higher than those of TSIIA and the physical mixing system. Based on these results, we conclude that the silica nanoparticle based SDs achieved complete dissolution, increased absorption rate, maintained drug stability, and showed improved oral bioavailability compared to TSIIA alone.Keywords: tanshinone IIA, solid dispersions, silica nanoparticles, in vitro

  16. Preparation and Evaluation of Novel Solid Chlorine Dioxide-based Disinfectant Powder in Single-pack

    Institute of Scientific and Technical Information of China (English)

    MIN ZHU; LI-SHI ZHANG; XIAO-FANG PEI; XIN XU

    2008-01-01

    Objective To prepare and evaluate novel chlorine dioxide-based disinfectant powder in single-pack that is more convenient for use and iransportation.Methods Orthogonal experiment was performed to determine the recipe of the disinfectant powder.Stability test,suspension quantitative bactericidal test,simulation neld trial,and animal toxicity test were carried out to observe its bactericidal and toxicological effects.Results The orthogonal experiment showed thatthe type of water solution had no effect on the disinfectant powder and the best ratio of sodium chlorite to solid acid was 1:3.Ten grams of the disinfectant powder was fully dissolved in 20 mL water for 2 mill,and diluted to 500 mL in water.After 5-10 min,the concentration of chlorine dioxide(ClO2)solution was 266 mg/L to 276 mg/L.After stored at 54℃ for 14 d,the average concentration of ClO2 was decreased by 5.03%.Suspension quantitative bactericidal test showed that the average killing logarithm(KL)value for both Staphylococcus aureus and Escherichia coli in 100 mg/L ClO2 solution for 2 min was over 5.00.In simulation field trial,the average descending KL value for Escherichia coli in the solution containing 100 mg/L ClO2 for 5min was ovcr 3.00.The mouse acute LD50 in the solution 5 times exceeded 5000 mg/kg.The disinfectant powder was not toxic and irritativeto rabbit skin and had nomutagenic effect on mouse marrow polychrornafic erythrocytes(PCE).Conclusion The stability and bactericidal efficacy of solid chlorine dioxide-based disinfectant powder in single-pack are good.The solution containing 100mg/L ClO2 can kill vegetative forms of bacteria.The concenwation of ClO2 on the disinfecting surface of objects is 100mg/L.The disinfectant powder is not toxic and irritative.

  17. Compound antimalarial ethosomal cataplasm: preparation, evaluation, and mechanism of penetration enhancement

    Directory of Open Access Journals (Sweden)

    Shen S

    2015-06-01

    Full Text Available Shuo Shen, Shu-Zhi Liu, Yu-Shi Zhang, Mao-Bo Du, Ai-Hua Liang, Li-Hua Song, Zu-Guang Ye Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, People’s Republic of China Abstract: Malaria is still a serious public health problem in some parts of the world. The problems of recurrence and drug resistance are increasingly more serious. Thus, it is necessary to develop a novel antimalarial agent. The objectives of this study were to construct a novel compound antimalarial transdermal nanosystem–ethosomal cataplasm, to investigate its characteristics and efficiency, and to systematically explore the penetration-enhancing mechanisms of ethosomal cataplasm. Artesunate-loaded ethosomes and febrifugine-loaded ethosomes were prepared, and their characteristics were evaluated. Drug-loaded ethosomes were incorporated in the matrix of cataplasm to form the compound antimalarial ethosomal cataplasm. With the help of ethosomal technology, the accumulated permeation quantity of artesunate significantly increased at 8 hours after administration, which was 1.57 times as much as that of conventional cataplasm. Soon after administration, the ethosomal cataplasm could make a large quantity of antimalarial drug quickly penetrate through skin, then the remaining drug in the ethosomal cataplasm could be steadily released. These characteristics of ethosomal cataplasm are favorable for antimalarial drugs to kill Plasmodium spp. quickly and prevent the resurgence of Plasmodium spp. As expected, the ethosomal cataplasm showed good antimalarial efficiency in this experiment. The negative conversion rates were 100% and the recurrence rates were 0% at all dosages. The mechanism of penetration enhancement of the ethosomal cataplasm was systematically explored using an optics microscope, polarization microscope, and transmission electron microscopy. The microstructure, ultrastructure, and birefringent structure in skin were observed. Data

  18. Evaluation of the impact of birth preparation courses on the health of the mother and the newborn.

    Science.gov (United States)

    Mehdizadeh, Abolfazl; Roosta, Firuozeh; Chaichian, Shahla; Alaghehbandan, Reza

    2005-01-01

    This study was carried out to evaluate the impact of birth preparation courses on the health of the mother and the newborn. A randomized clinical trial study was carried out on 200 primigravid women younger than age 35 years with gestational age of 20 weeks. Subjects were randomly divided into two groups: control and trial. Birth preparation classes were introduced to the trial group in eight sessions during pregnancy, whereas the control group received only routine care. Measurable clinical, obstetrical, and neonatal advantages were monitored and compared in two groups. Patients in the trial group suffered from back and pelvic pain and headache significantly less often than patients in control group (two-tailed p(2) < 0.05). Preparation is significantly related to reduction in dystocic deliveries and cesarean section ( p(2) = 0.044). Antenatal preparation could play a major role in the health of mother and newborn during labor and postpartum. In addition, antenatal preparation should be introduced to all women during pregnancy as a national health policy in Iran.

  19. Certification of the contents (mass fractions) of Cd, Pb, Se, Cu, Zn, Fe and Mn in wholemeal flour and lyophilized brown bread reference materials. Wholemeal flour - CRM no. 189; brown bread - CRM no. 191

    Energy Technology Data Exchange (ETDEWEB)

    Wagstaffe, P.J.; Griepink, B.; Muntau, H.; Schramel, P.

    1987-01-01

    The report describes the preparation and certification of a wholemeal flour (CRM 189) and a lyophilised brown breas (CRM 191) for their contents (mass fractions) of elements of toxicological and nutritional importance: Cd, Pb, Se, Cu, Zn, Fe and Mn. Indicative values are also given for As, Ca, Cl, Cr, Hg, Mg, Na, Ni, P and K. Details are given of a preliminary intercomparison of methods for these elements in a wholemeal flour sample, homogeneity and stability studies on the two reference materials and the results and evaluation of the certification exercise which involved 21 European Laboratories. Summaries of the certification methods are also presented. The report concludes with a discussion of the most common sources of error in determining the elements of interest and the steps to be taken to control them. With 7 figs., 28 tabs.

  20. Experimental evaluation of eco-friendly flocculants prepared from date palm rachis

    Institute of Scientific and Technical Information of China (English)

    Ramzi Khiari; Sonia Dridi-Dhaouadi; Chadlia Aguir; Mohamed Farouk Mhenni

    2010-01-01

    Sodium carboxymethylcellulose (CMCNa) is an anionic water soluble polyelectrolyte widely used in many industrial sectors including food,textiles,papers,adhesives,paints,pharmaceuticals,cosmetics and mineral processing.CMCNa was produced by chemical modification of cellulose,and represents many advantages:natural,renewable,non-toxic and biodegradable.In this study,different kinds of CMCNa,prepared from an agricultural waste date palm rachis,were tested as eco-friendly flocculants for drinking water treatment and their performances as flocculants in turbidity removal enhancement were assessed.The prepared materials were characterized by the degree of substitution (DS) and polymerisation (DP).The study of the effect of some experimental parameters on the coagulation-flocculation performance,using the prepared materials combined with aluminium sulphate (as coagulant),showed that the best conditions for turbidity treatment were given for pH 8,coagulant dose 20 mg/L,flocculant concentration of 100 mg/L and stirring velocity (during the flocculation step) of 30 r/min.Under the optimum conditions,the turbidity removal using CMCNa,prepared from raw material,was about 95%.A comparison study between the flocculation performance of a commercial anionic flocculant (A100 PWG:polyacrylamide) and that of the prepared CMCNa showed that the performance of the waste-based flocculant with a DS of 1.17 and a DP of 480 was 10% better than that achieved by the commercial one.

  1. Forest certification in Russia: development, current state and problems

    Directory of Open Access Journals (Sweden)

    Lukashevich Victor

    2016-03-01

    Full Text Available The article analyzes the development of voluntary forest certification by the Forest Stewardship Council (FSC system in Russia. The article is based on the review of diverse information sources, analysis of the reports of timber processing enterprises, personal observations during certification audits, discussions in workgroups, and information collected at training courses. We evaluated the present state of voluntary forest certification in Russia, analyzed non-compliances of the activity of Russian wood processing enterprises with the national standard FSC-STD-RUS-V6-1-2012 and indicated possible reasons for non-fulfillment of the requirements. We also presented problems in the development of forest certification in Russia and possible ways for its further development.

  2. Life Cycle Assessment and the Environmental Certification Systems of Buildings

    Directory of Open Access Journals (Sweden)

    Cristiane Bueno

    2013-09-01

    Full Text Available The life cycle assessment (LCA is an important methodology for the assessment of building components and systems and should be used within the building environmental performance certification systems. The present paper reports on the state of the art of LCA as a tool for the assessment of building components and analyzes its application in the environmental certification systems of building. The analysis of the existing systems for the environmental certification of buildings has revealed that among the four main and most employed systems of environmental certification of buildings currently used, Green Globes is the only one which uses the LCA methodology to evaluate the environmental performance of building components. The other systems use the assessment of building components by the recognition of product attributes, such as cost, durability, renewability and recycled content. The weakness of the attributes approach lies in the fact that these attributes are treated in isolation and lack the whole concept of impact.

  3. PULSE Pilot Certification Results

    Directory of Open Access Journals (Sweden)

    Pamela Pape-Lindstrom

    2015-08-01

    Full Text Available The pilot certification process is an ambitious, nationwide endeavor designed to motivate important changes in life sciences education that are in line with the recommendations of the 2011 Vision and Change Report: A Call to Action (American Association for the Advancement of Science [AAAS], 2011.  It is the goal of the certification process to acknowledge departments that have progressed towards full implementation of the tenets of Vision and Change and to motivate departments that have not begun to adopt the recommendations to consider doing so.  More than 70 life science departments applied to be part of the pilot certification process, funded by a National Science Foundation grant, and eight were selected based on initial evidence of transformed and innovative educational practices.  The programs chosen represent a wide variety of schools, including two-year colleges, liberal-arts institutions, regional comprehensive colleges, research universities and minority serving institutions.  Outcomes from this pilot were released June 1, 2015 (www.pulsecommunity.org, with all eight programs being recognized as having progressed along a continuum of change.  Five levels of achievement were defined as PULSE Pilot Progression Levels.  Of the eight departments in the pilot, one achieved “PULSE Progression Level III: Accomplished”.  Six departments achieved “PULSE Progression Level II: Developing” and one pilot department achieved “PULSE Progression Level I: Beginning”.  All of the schools have made significant movement towards the recommendations of Vision and Change relative to a traditional life sciences curriculum.  Overall, the response from the eight pilot schools has been positive. 

  4. Evaluation of extraction methods for preparative scale obtention of mangiferin and lupeol from mango peels (Mangifera indica L.).

    Science.gov (United States)

    Ruiz-Montañez, G; Ragazzo-Sánchez, J A; Calderón-Santoyo, M; Velázquez-de la Cruz, G; de León, J A Ramírez; Navarro-Ocaña, A

    2014-09-15

    Bioactive compounds have become very important in the food and pharmaceutical markets leading research interests seeking efficient methods for extracting these bioactive substances. The objective of this research is to implement preparative scale obtention of mangiferin and lupeol from mango fruit (Mangifera indica L.) of autochthonous and Ataulfo varieties grown in Nayarit, using emerging extraction techniques. Five extraction techniques were evaluated: maceration, Soxhlet, sonication (UAE), microwave (MAE) and high hydrostatic pressures (HHP). Two maturity stages (physiological and consumption) as well as peel and fruit pulp were evaluated for preparative scale implementation. Peels from Ataulfo mango at consumption maturity stage can be considered as a source of mangiferin and lupeol using the UEA method as it improves extraction efficiency by increasing yield and shortening time. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Evaluating the impact of a fungal-origin chitosan preparation on Brettanomyces bruxellensis in the context of wine aging

    Directory of Open Access Journals (Sweden)

    Tiziana Nardi

    2014-12-01

    Full Text Available Brettanomyces bruxellensis and the consequences of its development in wines are a continuous threat for wine quality. In this context, chitosan of fungal origin was introduced as a new tool to control B. bruxellensis in the context of winemaking. Recent studies have showed the impact of a fungal origin chitosan application on wines contaminated with B. bruxellensis, leading to the elimination of B. bruxellensis cells. In these studies, the chitosan preparation was added, the wine racked off after 10 days and the efficiency of the treatment was evaluated in a short delay after the treatment. This study focused on the evaluation of the impact of different addition protocols of an enological chitosan preparation on B. bruxellensis population evolution and volatile phenols content along the aging, up to 9 months. The results confirm the interest of fungal origin chitosan as a preventive tool to control B. bruxellensis in the context of wine aging.

  6. Evaluations of different domestic hot water preparing methods with ultra-low-temperature district heating

    DEFF Research Database (Denmark)

    Yang, Xiaochen; Li, Hongwei; Svendsen, Svend

    2016-01-01

    of Legionella in the DHW (domestic hot water) and assure the comfortable temperature, all substations were installed with supplementary heating devices. Detailed measurements were taken in the substations, including the electricity demand of the supplementary heating devices. To compare the energy and economic...... performance of the substations, separate models were built based on standard assumptions. The relative heat and electricity delivered for preparing DHW were calculated. The results showed that substations with storage tanks and heat pumps have high relative electricity demand, which leads to higher integrated...... costs considering both heat and electricity for DHW preparation. The substations with in-line electric heaters have low relative electricity usage because very little heat is lost due to the instantaneous DHW preparation. Accordingly, the substations with in-line electric heaters would have the lowest...

  7. Evaluation of the clenbuterol imprinted monolithic column prepared by reversible addition-fragmentation chain transfer polymerization

    Institute of Scientific and Technical Information of China (English)

    Mamat Turson; Xiao Lei Zhuang; Hui Na Liu; Ping Jiang; Xiang Chao Dong

    2009-01-01

    To make more homogenous organic monolithic structure,reversible addition-fragmentation chain transfer(RAFT)process was employed in the synthesis of the clenbuterol imprinted polymer.In the synthesis,the influence of synthetic conditions on the polymer structure and separation efficiency was studied.The result demonstrated that the imprinted columns prepared with RAFT process have higher column efficiency and selectivity than the columns prepared with conventional polymerization in the present study,which may result from the higher surface area,smaller pore size and the narrower globule size distribution in their structures.The result indicated that RAFT polymerization provided better conditions for the clenbuterol imprinted monolithic polymer preparation.

  8. Using a Web-Based Resource to Prepare Students for Fieldwork: Evaluating the Dark Peak Virtual Tour

    Science.gov (United States)

    McMorrow, Julia

    2005-01-01

    This paper reports on development of a Dark Peak website and its use to prepare first-year geography students for a one-day physical geography field course in the south Pennines. The Virtual Tour (VT) component of the website is the main focus of this paper. Pre- and post-fieldwork evaluations of the first version of the VT by 195 students are…

  9. Using a Web-Based Resource to Prepare Students for Fieldwork: Evaluating the Dark Peak Virtual Tour

    Science.gov (United States)

    McMorrow, Julia

    2005-01-01

    This paper reports on development of a Dark Peak website and its use to prepare first-year geography students for a one-day physical geography field course in the south Pennines. The Virtual Tour (VT) component of the website is the main focus of this paper. Pre- and post-fieldwork evaluations of the first version of the VT by 195 students are…

  10. Evaluation of roll compaction as a preparation method for hydroxypropyl cellulose-based matrix tablets

    Directory of Open Access Journals (Sweden)

    Imjak Jeon

    2011-01-01

    Full Text Available Roll compaction was applied for the preparation of hydroxypropyl cellulose (HPC-based sustained-release matrix tablets. Matrix tablets made via roll compaction exhibited higher dosage uniformity and faster drug release than direct-compacted tablets. HPC viscosity grade, roll pressure, and milling speed affected tablet properties significantly. Roll compaction seems to be an adequate granulation method for the preparation of HPC-based matrix tablets due to the simplicity of the process, less handling difficulty from HPC tackiness as well as easier particle size targeting. Selecting the optimum ratio of plastic excipients and the particle size of starting materials can however be critical issues in this method.

  11. MCSD certification toolkit (exam 70-483) programming in C#

    CERN Document Server

    Covaci, Tiberiu; Varallo, Vincent; O'Brien, Gerry

    2013-01-01

    A perfectly crafted prep guide that prepares you for the MCSD 70-483 The MCSD 70-483 exam is the entry-level Microsoft certification exam for C# developers and this must-have resource offers essential coverage of the exam that will test your competency in C# programming. Each chapter covers one of the core subject domains that comprise the exam. Among the authors are experienced trainers who advised Microsoft on the development of its certification programs, affording them a unique understanding of both the objectives and what it takes to master them. This invaluable knowledge is p

  12. Device-independent certification of high-dimensional quantum systems.

    Science.gov (United States)

    D'Ambrosio, Vincenzo; Bisesto, Fabrizio; Sciarrino, Fabio; Barra, Johanna F; Lima, Gustavo; Cabello, Adán

    2014-04-11

    An important problem in quantum information processing is the certification of the dimension of quantum systems without making assumptions about the devices used to prepare and measure them, that is, in a device-independent manner. A crucial question is whether such certification is experimentally feasible for high-dimensional quantum systems. Here we experimentally witness in a device-independent manner the generation of six-dimensional quantum systems encoded in the orbital angular momentum of single photons and show that the same method can be scaled, at least, up to dimension 13.

  13. An Assessment of National EMT Certification Among Enlisted Military Medics.

    Science.gov (United States)

    Harper, Stephen; Crowe, Remle; Bentley, Melissa; Kharod, Chetan; Walrath, Benjamin

    2017-03-01

    Navy Hospital Corpsmen (HMs) are the Navy equivalent to Emergency Medical Technicians (EMTs) both in-garrison and on the battlefield. In 2000, the Emergency Medical Services (EMS) Education Agenda for the Future highlighted the need for a single certification agency to provide consistent evaluation of entry level competence for each nationally recognized EMS provider level. Administered by the National Registry of EMTs (NREMT), National EMT Certification is currently utilized by 46 states, the District of Columbia, four territories, and six federal organizations as part of their processes for granting licensure. Unlike the Air Force (USAF) and Army (USA), the Navy (USN) does not require National EMT Certification to perform the duties equivalent to a civilian EMT. Our objective is to describe the number of USN HMs, USAF medics, and USA combat medics who have obtained National EMT Certification from 2007 through 2014. Results from all USN HMs, USAF medics, and USA combat medics who tested between January 1, 2007 and December 31, 2014 were queried from the NREMT database. Descriptive statistics were calculated based on a retrospective review of prospectively collected testing data. During the study period, 89,136 Military Service Members received their EMT certification from the NREMT. The breakdown of the total and percent of total is; USA Combat Medics (n = 69,761; 78.3%), USAF Medics (n = 16,195; 18.1%), and USN HMs (n = 3,180; 3.6%). Approximately 4,000 HMs graduate yearly from the Department of Defense Medical Education and Training Campus at Fort Sam Houston, Texas and 253 HMs obtained certification in 2014. About 6.3% (253/4,000) HMs obtained National EMT Certification in 2014, which is a nationally recognized standard for entry-level competence utilized by civilian EMTs and other branches of the military. More information about those HMs that obtain certification may help Commanders maximize the number of HMs obtaining certification. Mandating National EMT

  14. Are you considering organic certification?

    Science.gov (United States)

    Organic certification was developed in recognition of the necessity for consistent standards across the U.S. for the benefit of producers, processors, wholesalers, retailers, and consumers. Prior to establishment of federal guidelines (National Organic Program) for organic certification in 2002, a ...

  15. Evaluation of error sources in a gravimetric technique for preparation of a reference gas mixture (carbon dioxide in synthetic air).

    Science.gov (United States)

    Matsumoto, Nobuhiro; Shimosaka, Takuya; Watanabe, Takuro; Kato, Kenji

    2008-07-01

    One method of preparing a primary reference gas mixture is the gravimetric blending method. Uncertainty of a few mg in mass measurements is unavoidable when preparing reference gas mixtures under current laboratory conditions with our facilities, equipment, and materials. There are many sources of errors when using this method. In this study, several sources of errors were re-evaluated for our process for preparation of carbon dioxide in synthetic air. As a consequence of the re-evaluation, it was found that some sources of errors had significant effects on gravimetric concentrations of the gas mixtures. These sources are: (1) different masses of the reference cylinder and sample cylinder (an error in the readings of the electronic mass comparator), (2) leakage of the inner gas from valves of the cylinders, and (3) cooling of the gas cylinder caused by filling with high-pressure liquefied carbon dioxide gas. When the mass measurements were performed under uncontrolled conditions, the errors due to sources (1), (2), and (3) were as high as 20 mg, 24 mg, and 13 mg, respectively. In this paper, the detailed results from re-evaluation of these sources of errors are discussed.

  16. Expectations of residents and tourists of agriculture-related certification systems: analysis of public perceptions

    Directory of Open Access Journals (Sweden)

    Yuta Uchiyama

    2017-06-01

    Conclusion: It is necessary to evaluate the GIAHS's contribution to tourism and industry when municipalities and international organizations manage the GIAHS sites. Considering the gaps between residents and tourists and taking measures to resolve them can contribute to managing certificated areas by collaboration of stakeholders. Regarding products aimed at branding, consumers need to be informed about the GIAHS as a regional certification in addition to product certification to share the historical and environmental characteristics of agricultural products.

  17. Preparation and evaluation of molecularly imprinted polymer liquid chromatography column for the separation of ephedrine enantiomers

    Directory of Open Access Journals (Sweden)

    Krishnamoorthy balamurugan

    2016-09-01

    Full Text Available In this study molecular imprinting technology was employed to prepare a specific affinity sorbent for the resolution of Ephedrine, a chiral drug. The molecularly imprinted polymer (MIP was prepared by non-covalent molecular imprinting with either (+ or (--Ephedrine ((R∗,S∗-2-(methylamino-1-phenylpropan-1-ol as the template. Methacrylic acid and ethylene glycol di-methacrylate were copolymerized in the presence of the template molecule. The bulk polymerization was carried out in chloroform with 2,2′-azobisisobutyronitrile as the initiator, at 5°C and under UV radiation. The resulting MIP was ground into powders, which were slurry packed into analytical columns. After removal of template molecules, the MIP-packed columns were found to be effective for the resolution of (±-Ephedrine racemates. The separation factor for the enantiomers ranged between 1.3 and 2.1 when the column was packed with MIP prepared with (+-Ephedrine as the template. A separation factor ranging from 1.3 to 2.6 could be achieved from the column packed with MIP, prepared with (--Ephedrine as the template. Although the separation factor was higher with that previously obtained from reversed-phase column chromatography following derivatization with a chiral agent, elution peaks were broader due to the heterogeneity of binding sites on the MIP particles and the possible non-specific interaction.

  18. Preparation and Evaluation of Children's Rights Education Curriculum: An Action Research Regarding on Protection Rights Module

    Science.gov (United States)

    Uçus, Sükran; Dedeoglu, Hakan

    2016-01-01

    Children's rights education is to enable children to gain the necessary social behaviors and essential knowledge for creating a democratic society that is based on respecting human rights. The purpose of this study was to investigate the preparation, application and assessment of a curriculum for teaching children's rights in elementary education.…

  19. Preparation and evaluation of monolithic molecularly imprinted stationary phase for S-naproxen

    Institute of Scientific and Technical Information of China (English)

    De-Miao Chen; Qiang Fu; Wei Du; Si-Juan Sun; Ping Huang; Chun Chang

    2011-01-01

    An S-naproxen(S-NAP)molecularly imprinted monolithic stationary phase(MIMSP)with specific recognition for S-NAP and naproxen(NAP)was prepared by in situ technique,utilizing 4-vinylpridine(4-VP)as a function monomer,ethylene glycol dimethacrylate(EDMA)as a

  20. Preparation and in vitro evaluation of rosin microcapsules: solvent evaporation technique.

    Science.gov (United States)

    Sheorey, D S; Dorle, A K

    1990-01-01

    Free flowing, spherical rosin microcapsules were prepared by a method based on phase separation by solvent evaporation. Bentonite was used to prevent the agglomeration and coalescence of the dispersed polymer droplets. The effect of varying the core to coat ratio on micrometric and dissolution properties has been described.

  1. Program Evaluation: A Study of the Impact of a Workforce Preparation Program

    Science.gov (United States)

    Blevins, Stephanie Strevels

    2010-01-01

    The Kentucky 4-H Reality Store, a workforce preparation program was established to educate youth about the importance of budgeting, setting goals, planning for careers, considering the future, preventing teen pregnancy, and abstaining from drug misuse. The program which has been administered to over 45,000 adolescents each year has never been…

  2. Preparation, characterization and evaluation of moisturizing and UV protecting effects of topical solid lipid nanoparticles

    Directory of Open Access Journals (Sweden)

    Shiva Golmohammadzadeh

    2012-12-01

    Full Text Available Solid lipid nanoparticles (SLN were recently proposed as carriers for various pharmaceutical and cosmetic actives. These lipid nanoparticles can act as moisturizers and physical sunscreens on their own. Therefore, the full potential of these carriers has yet to be determined. The present study was aimed to determine and compare moisturizing and UV-protecting effects of different solid lipid nanoparticles (SLN prepared by different solid lipids including Glyceryl monostearate (GMS, Precirol® (P and cetyl palmitate (CP as carrier systems of moisturizers and sunscreens. The influence of the size and matrix crystallinity of the solid lipids on the occlusive factor, skin hydration and UV-protection were evaluated by in vitro and in vivo methods. The SLN were prepared by high-shear homogenization and ultrasound methods. Size, zeta potential and morphological characteristics of the samples were assessed by transmission electron microscopy (TEM and thermotropic properties with differential scanning calorimetry (DSC technique. Results of the assessments showed that SLN-CP significantly increases skin hydration and UV-protection, compared to SLN-GMS and SLN-P. It was demonstrated that the size of SLN, crystallinity index of solid lipid in SLN and probably other mechanisms besides the occlusive factor can influence skin hydration and UV-protection indices. Furthermore, findings of the assessments demonstrated significant difference between in vitro and in vivo assessments regarding occlusive factor and moisturizing effects. Findings of the present study indicate that the SLN-CP could be a promising carrier for sunscreens and moisturizers.Nanopartículas lipídicas sólidas (NLS foram, recentemente, propostas como carreadores de vários ativos cosméticos e farmacêuticos. Essas nanopartículas lipídicas podem atuar como hidratantes e protetores solares físicos por si só. Assim sendo, determinou-se o potencial desses carreadores. Os objetivos do

  3. Guide energy certificate. Pt. 1. Energy demand certificate: Data acquisition residential buildings. 2. ed.; Leitfaden Energieausweis. T. 1. Energiebedarfsausweis: Datenaufnahme Wohngebaeude

    Energy Technology Data Exchange (ETDEWEB)

    Balkowski, Michael [Informations- und Beratungsinstitut fuer Energieeinsparung und Umweltschutz (IBEU) e.V., Dresden (Germany); Hausladen, Gerhard [Technische Univ. Muenchen (Germany); Kwapich, Thomas; Sager, Christina; Reichenberger, Romy [Deutsche Energie-Agentur GmbH, Berlin (Germany); Loga, Tobias [Institut Wohnen und Umwelt GmbH, Darmstadt (Germany); Jagnow, Kati [Ingenieurbuero fuer Energieberatung, Ostfalia (Germany); Fachhochschule Braunschweig-Wolfenbuettel, Wolfenbuettel (Germany)

    2009-12-15

    In the series 'Guide Energy Certificates', the German Energy Agency (Berlin, Federal Republic of Germany) reports on all necessary fundamentals for the issue of energy certificates for specialists. The first part describes the correct approach of data acquisition for the demand-oriented energy document for residential buildings. The rules of the proclamations which can be applied as well as the simplifications permissible according to EnEV 2009 are presented. Practical hints for the preparing of energy certificates are given.

  4. Field Evaluations Test Plan for Validation of Alternative Low-Emission Surface Preparation/Depainting Technologies for Structural Steel

    Science.gov (United States)

    Lewis, Pattie

    2005-01-01

    Headquarters National Aeronautics and Space Administration (NASA) chartered the Acquisition Pollution Prevention (AP2) Office to coordinate agency activities affecting pollution prevention issues identified during system and component acquisition and sustainment processes. The primary objectives of the AP2 Office are to: (1) Reduce or eliminate the use of hazardous materials (HazMats) or hazardous processes at manufacturing, remanufacturing, and sustainment locations. (2) Avoid duplication of effort in actions required to reduce or eliminate HazMats through joint center cooperation and technology sharing. To achieve a substrate condition suitable for the application of a coating system, both new and old (in-situ) substrates must undergo some type of surface preparation and/or depainting operation to ensure adhesion of the new coating system. The level of cleanliness or anchor profile desired is typically a function of the type of coating to be applied and the specification being adhered to. In high performance environments, cleanliness and surface profile requirements for carbon steel (the dominant substrate for facilities, structures and equipment) dictates the use of abrasive media. Many of the abrasive media currently used across NASA and Air Force Space Command (AFSPC) installations generate large quantities of fugitive particulate emissions and waste. The high quantities of airborne dust and waste generated from these operations pose significant environmental concern. Efforts to contain emissions and the reduce quantity of waste generated have significant implications on project cost; this is often a deterrent to engaging in maintenance activities. In response to recent technological developments and NASA's and AFSPC's need to undertake environmentally conscious corrosion prevention projects, a review of the industry needs to be undertaken to evaluate surface preparation technologies (materials and processes) for embrace. This project will identify, evaluate

  5. Evaluation of Diagnostic Sensitivity of Wet Preparation Microscopy Using KOH for Detection of Fungal Agents from Keratitis Patients

    Directory of Open Access Journals (Sweden)

    Neeta D Khokhar, Summaiya A Mulla, Latika N Shah, Geeta M Vaghela

    2013-01-01

    Full Text Available Background: Keratitis is a common ophthalmic condition mostly caused by fungi. Apart from fungal culture, wet preparation using 10% Potassium hydroxide (KOH for microscopic detection of fungal elements is a rapid and accurate method of laboratory diagnosis. Purpose: This prospective study was undertaken in order to evaluate the diagnostic sensitivity of wet preparation microscopy using KOH for detection of fungal agents from keratitis patients. Methodology: 103 samples of clinically suspected patients of keratitis attending tertiary care hospital between march 2010 and june 2011 were included. Samples like corneal swabs, corneal scrapings, corneal button, and corneo-scleral rim were collected aseptically after slit lamp examination, then transported to microbiology laboratory. Samples were processed for direct microscopy (gram stain and 10% KOH wet mount preparation and culture. Culture positive isolates were identified based on morphology and standard biochemical tests. Data entry and analysis was done statistically. Results: From 103 samples, fungal culture was positive in 12%. Different fungus isolates include Aspergillus flavus (67% and Candida spp (25% were the leading fungi followed by Curvularis (8%. Direct microscopical examination using KOH wet preparation and gram’s stain had detected fungal elements in 83% and 75% samples respectively against culture results. Conclusion: Aspergillus flavus (67%, Candida spp (25% and Curvularia spp (8% was most common cause of fungal keratitis. Wet mount with KOH can be relied upon as the single most important screening tool for rapid diagnosis of fungal corneal ulcer and treatment should be dispensed on its basis.

  6. Preparation and biocompatibility evaluation of bioactive glass-forsterite nanocomposite powder for oral bone defects treatment applications.

    Science.gov (United States)

    Saqaei, Mahboobe; Fathi, Mohammadhossein; Edris, Hossein; Mortazavi, Vajihesadat

    2015-11-01

    Bone defects which emerge around dental implants are often seen when implants are placed in areas with insufficient alveolar bone, in extraction sockets, or around failing implants. Bone regeneration in above-mentioned defects using of bone grafts or bone substitutes may cure the long-term prognoses of dental implants. Biocompatibility, bioactivity and osteogenic properties are key factors affecting the applications of a bone substitute. This study was aimed at preparation, characterization, biocompatibility and bioactivity evaluation of the bioactive glass-forsterite nanocomposite powder as a desired candidate for oral bone defect treatments. Nanocomposite powders containing 58S bioactive glass and different amounts of forsterite nanopowder were synthesized in situ by sol-gel technique. Characterization of the prepared nanocomposite powders and their cytotoxicity assessment was performed via MTT test. Bioactivity assessment was done by immersing the prepared powder in the simulated body fluid (SBF). Results showed that nanocomposite powders containing forsterite with crystallite size of 20-50nm were successfully fabricated by calcination at 600°C. The prepared bioactive glass-forsterite nanocomposite powders revealed high in vitro biocompatibility; besides, the nanocomposite containing 20wt.% forsterite showed a substantial increase in the cell viability compared with control groups. During immersion in SBF, the formation of apatite layer confirmed the bioactivity of bioactive glass-forsterite nanocomposite powders. According to the results, the fabricated nanocomposite powders can be introduced as a promising candidate for oral bone imperfection treatments and hard tissue mend.

  7. Physical chemical and citotoxic evaluation of highly diluted solutions of Euphorbia tirucalli L. prepared through the fifty milesimal homeopathic method

    Directory of Open Access Journals (Sweden)

    Carlos Renato Zacharias

    2010-07-01

    Full Text Available Background: although Hahnemann described the fifty-milesimal (LM method in the 6th edition of the Organon of the Medical Art, very little research has been carried out on the physical chemical properties of these homeopathic preparations. Furthermore, there is still no evidence allowing for the correlation between the alleged physical chemical properties and the biological effects of high dilutions. Aims: to evaluate physical chemical characteristics of LM preparations including electrical conductivity, pH and refraction index, and their effect on biological experimental models. Materials and methods: preparations tested for physical chemical analysis were dilutions 1 lm to 10 lm of Euphorbia tirucalli L. prepared from the latex and the juice of the plant. To rule the seasonal characteristics of this plant, 2 different populations were used, one collected in June 2007 and the other in May 2008. Furthermore, the cytotoxic effect of Euphorbia tirucalli 5 lm was tested on human breast cancer cells (MCF7 through MTT assay. Some differences among the two collections were observed. However, any clear correlation could be observed between physical chemical properties and biological activity.

  8. Sludge batch 9 (SB9) acceptance evaluation. Radionuclide concentrations in tank 51 SB9 qualification sample prepared at SRNL

    Energy Technology Data Exchange (ETDEWEB)

    Bannochie, C. J. [Savannah River Site (SRS), Aiken, SC (United States); Diprete, D. P. [Savannah River Site (SRS), Aiken, SC (United States); Pareizs, J. M. [Savannah River Site (SRS), Aiken, SC (United States)

    2016-02-10

    Presented in this report are radionuclide concentrations required as part of the program of qualifying Sludge Batch 9 (SB9) for processing in the Defense Waste Processing Facility (DWPF). The SB9 material is currently in Tank 51 and has been washed and prepared for transfer to Tank 40. The acceptance evaluation needs to be completed prior to the transfer of the material in Tank 51 to Tank 40. The sludge slurry in Tank 40 has already been qualified for DWPF processing and is currently being processed as Sludge Batch 8 (SB8). The radionuclide concentrations were measured or estimated in the Tank 51 SB9 Washed Qualification Sample prepared at Savannah River National Laboratory (SRNL). This sample was prepared from a three liter sample of Tank 51 sludge slurry (HTF-51-15-81) taken on July 23, 2015. The sample was delivered to SRNL where it was initially characterized in the Shielded Cells. Under the direction of Savannah River Remediation (SRR) it was then adjusted per the Tank Farm washing strategy as of October 20, 2015. This final slurry now has a composition expected to be similar to that of the slurry in Tank 51 after final preparations have been made for transfer of that slurry to Tank 40.

  9. Sludge batch 9 (SB9) accepance evaluation: Radionuclide concentrations in tank 51 SB9 qualification sample prepared at SRNL

    Energy Technology Data Exchange (ETDEWEB)

    Bannochie, C. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Diprete, D. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Pareizs, J. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2016-03-01

    Presented in this report are radionuclide concentrations required as part of the program of qualifying Sludge Batch 9 (SB9) for processing in the Defense Waste Processing Facility (DWPF). The SB9 material is currently in Tank 51 and has been washed and prepared for transfer to Tank 40. The acceptance evaluation needs to be completed prior to the transfer of the material in Tank 51 to Tank 40. The sludge slurry in Tank 40 has already been qualified for DWPF processing and is currently being processed as Sludge Batch 8 (SB8). The radionuclide concentrations were measured or estimated in the Tank 51 SB9 Washed Qualification Sample prepared at Savannah River National Laboratory (SRNL). This sample was prepared from a three liter sample of Tank 51 sludge slurry (HTF-51-15-81) taken on July 23, 2015. The sample was delivered to SRNL where it was initially characterized in the Shielded Cells. Under the direction of Savannah River Remediation (SRR) it was then adjusted per the Tank Farm washing strategy as of October 20, 2015. This final slurry now has a compositioniv expected to be similar to that of the slurry in Tank 51 after final preparations have been made for transfer of that slurry to Tank 40.

  10. Preparation of iron bound succinylated milk protein concentrate and evaluation of its stability.

    Science.gov (United States)

    Shilpashree, B G; Arora, Sumit; Sharma, Vivek; Bajaj, Rajesh Kumar; Tomar, S K

    2016-04-01

    Major problems associated with the fortification of soluble iron salts include chemical reactivity and incompatibility with other components. Milk protein concentrate (MPC) are able to bind significant amount of iron due to the presence of both casein and whey protein. MPC in its native state possess very poor solubility, therefore, succinylated derivatives of MPC (succ. MPC) were also used for the preparation of protein-iron complex. Preparation of the complex involved centrifugation (to remove insoluble iron), ultrafiltration (to remove unbound iron) and lyophilisation (to attain in dry form). Iron binding ability of MPC enhanced significantly (Piron from both varieties of complexes was monitored under different conditions encountered during processing. Higher stability (Piron was observed in succ. MPC-iron complex than native protein complex. This method could be adopted for the production of stable iron enriched protein, an organic iron source.

  11. Preparation and Evaluation in vitro of Salicylic Acid-pachyman Nanoparticles

    Institute of Scientific and Technical Information of China (English)

    ZHANG Junbo; SHAO Huili; XIAO Yuling; ZHU Yuanchun; LIANG Shucai

    2011-01-01

    Pachyman based nanoparticles loading salicylic acid as model drug(SA-PNPs)were prepared by an inverse microemulsion crosslinking approach using epichlorohydrin(ECH)as crosslinker.The effects of crosslinking reaction time,initial volume ratio of oil to aqueous phase and dosage of crosslinker on the particle size of SA-PNPs were optimized by orthogonal experimental design.SA-PNPs prepared under the optimal conditions had the average size of 230 nm and high encapsulation efficiency of 90%.The in vitro drug release was also investigated and the release data were analyzed using zero order,first order and Higuchi's kinetics model.According to the determined coefficients,release data fitted to Higuchi's model,which suggested that the release of SA from SA-PNPs in phosphate buffer(pH 7.4)was diffusion controlled release.The experimental results indicated that pachyman possesses a promising potential to be applied as nanocarriers for controlled drug release.

  12. Ionotropically gelled novel hydrogel beads: Preparation, characterization and in vitro evaluation

    OpenAIRE

    2011-01-01

    Prolonged release drug delivery system of stavudine was made by ionotropic gelation and polyelectrolyte complexation technique. Cross-linking reinforced chitosan-gellan complex beads were prepared by gelation of anionic gellan gum, the primary polymer, with oppositely charged counter ion to form beads which were further complexed with chitosan as a polyelectrolyte. The effect of this polymer on release profile of drug was studied. Beads without chitosan complexation were also made. The reacti...

  13. Evaluation of Management System Effectiveness in the Preparation of the Aircraft for Flight in Faulty Conditions

    Directory of Open Access Journals (Sweden)

    Bogdane Ruta

    2015-11-01

    Full Text Available Most flight delays in aviation enterprises are related to air traffic management and technical centers. This can happen for various reasons: untimely removal of defects, lack of spare parts, deficiencies in maintenance scheduling, etc. Another reason may be inefficient management in the system of preparing the aircraft for departure. The article suggests a possible option of such an assessment as well as the results obtained from the use of this methodology applied to a specific airline.

  14. Preparation and Biological Evaluation of [18F]Ta-Gluc-TOCA

    Institute of Scientific and Technical Information of China (English)

    WEN; Kai; CHEN; Bao-jun; GUO; Fei-hu; LIANG; Ji-xin; CUI; Hai-ping

    2012-01-01

    <正>The purpose of this research was the synthesis of the glycosylated octreotide derivative quickly and easily, development of a method for 18F labeled peptides by the click chemistry, and exploring the feasibility using 18F labeled glycosylated octreotide derivatives as a somatostatin receptor positive tumor probe. Firstly, the 2-18F-azide ethane precursor compound was prepared by nucleophilic substitution, then

  15. Evaluation of Peel Ply Surface Preparation of Composite Surfaces for Secondary Bonding

    Science.gov (United States)

    2013-09-01

    15 epoxy resin (Applied Poleramic Inc., Benicia, CA) were prepared by the Vacuum Assisted Resin Transfer Molding ( VARTM ) process. Two methods were...viscosity two-phase toughened epoxy resin matrix designed for VARTM processes [8]. The rheological properties of SC-15 have previously been...material system fabricated by the VARTM process ranges between 49 - 52 % with a typical void volume of ~ 1 %, also previously established. Nominal cured

  16. Preparation, characterization, and in vivo evaluation of intranasally administered liposomal formulation of donepezil

    Directory of Open Access Journals (Sweden)

    Al Asmari AK

    2016-01-01

    Full Text Available Abdulrahman K Al Asmari,1 Zabih Ullah,1 Mohammad Tariq,1 Amal Fatani21Department of Research, Prince Sultan, Military Medical City, 2Department of Pharmacy, King Saud University, Riyadh, Saudi ArabiaAbstract: The adequate amount of drug delivery to the brain in neurological patients is a major problem faced by the physicians. Recent studies suggested that intranasal administration of liposomal formulation may improve the drug delivery to the brain. In the present study, an attempt was made to study the brain bioavailability of commonly used anti-Alzheimer drug donepezil (DNP liposomal formulation by intranasal route in rats. We adopted the thin layer hydration technique for the preparation of liposomes by using cholesterol, polyethylene glycol, and 1,2-distearyl-sn-glycero-3-phosphocholine (DSPC. The prepared liposomes were characterized by determining particle size, shape, surface morphology, zeta potential, encapsulation efficiency, and in vitro release of DNP. The pharmacokinetic parameters of liposomal DNP in plasma and brain of rats were determined following oral and nasal administration. The results of this study showed that the DNP liposomal formulation was stable with a consistent size (102±3.3 nm and shape. The prepared liposomes showed high encapsulation efficiency (84.91%±3.31% and sustained-release behavior. The bioavailability of DNP in plasma and brain increased significantly (P<0.05 after administration of liposomal formulation by the intranasal route. Histopathological examination showed that the formulation was safe and free from toxicity. It can be concluded that the nasal administration of liposomal preparation may provide an efficient and reliable mode of drug delivery to the central nervous system.Keywords: donepezil, intranasal, liposomes, bioavailability, blood–brain barrier

  17. Clinical Evaluation of Different Pre-impression Preparation Procedures of Dental Arch

    Science.gov (United States)

    Arora, Nitin; Arora, Monika; Gupta, Naveen; Agarwal, Manisha; Verma, Rohit; Rathod, Pankaj

    2015-01-01

    Background: Bubbles and voids on the occlusal surface impede the actual intercuspation and pre-impression preparation aims to reduce the incidence of air bubbles and voids as well as influences the quality of occlusal reproduction and actual clinical intercuspation in the articulator. The study was undertaken to determine the influence of different pre-impression preparation procedures of antagonistic dental arch on the quality of the occlusal reproduction of the teeth in irreversible hydrocolloid impressions and to determine most reliable pre-impression preparation method to reduce the incidence of air bubbles. Materials and Methods: A total of 20 subjects were selected having full complement of mandibular teeth from second molar to second molar with well demarcated cusp height. 200 impressions were made with irreversible hydrocolloid material. The impressions were divided into five groups of 40 impressions each and each group had one specific type of pre-impression preparation. All the impressions were poured in die stone. A stereomicroscope with graduated eyepiece was used to count the number of bubbles on the occlusal surface of premolars and molars. The mean and standard deviations were calculated for each group. Mann–Whitney U-test was applied to find the significant difference between different groups. Results: Least bubbles were found in the group in which oral cavity was dried by saliva ejector and fluid hydrocolloid was finger painted onto the occlusal surfaces immediately before the placement of impression tray in the mouth. Conclusion: It was found that finger painting the tooth surfaces with fluid hydrocolloid immediately before the placement of loaded impression tray in the mouth was the most reliable method. The oral cavity can be cleared more easily of excess saliva by vacuum suction rather than by use of an astringent solution. PMID:26229376

  18. Preparation and In Vitro Evaluation of Ethylcellulose and Polymethacrylate Resins Loaded Microparticles Containing Hydrophilic Drug

    Directory of Open Access Journals (Sweden)

    Satish Pandav

    2014-01-01

    Full Text Available Objective. The purpose of the recent study was to prepare and estimate sustained release of Ethylcellulose (300 cps and Eudragit (RS 100 and RL 100 microparticles containing Propranolol hydrochloride used as a treatment of cardiovascular system, especially hypertension. Method. Propranolol hydrochloride was microencapsulated with different polymers (Ethylcellulose, Eudragit RS, and Eudragit RL using modified hydrophobic (O/O solvent evaporation method using 1 : 1 combination of acetone and isopropanol as the internal phase. Obtained microparticles were showing higher batch yield with higher encapsulation efficiency. Microparticles were prepared with different ratios of 1 : 1, 1 : 3, 1 : 5, and 1 : 7 (%, wt/wt using span 80 (%, v/v as a surfactant. Results. The influence of formulation factors like drug: polymer ratio, internal phase, and type of polymers on obtained microparticles was characterized with respect to particle size distribution, encapsulation efficiency, percentage yield, FTIR, and FE-SEM. Higher encapsulation efficiencies were obtained with various polymers like Ethylcellulose (96.63 ± 0.5 compared to Eudragit RS 100 (83.70 ± 0.6 and RL 100 (89.62 ± 0.6. The in vitro release study was characterized by initial burst. Conclusion. The result of study displays that Ethylcellulose and Eudragit loaded microparticles of Propranolol hydrochloride can be effectively prepared using modified hydrophobic emulsification solvent evaporation technique. Therefore, the modified hydrophobic emulsion technique can also be applied to the preparation of microparticles for low molecular weight and highly water soluble drugs.

  19. Preparation and Evaluation of Dental Resin with Antibacterial and Radio-Opaque Functions

    OpenAIRE

    Pekka K. Vallittu; Lippo V. J. Lassila; Jingwei He; Eva Söderling

    2013-01-01

    In order to prepare antibacterial and radio-opaque dental resin, a methacrylate monomer named 2-Dimethyl-2-dodecyl-1-methacryloxyethyl ammonium iodine (DDMAI) with both antibacterial and radio-opaque activities was added into a 2,2-bis[4-(2-hydroxy-3-methacryloyloxypropyl)-phenyl]propane (Bis-GMA)/methyl methacrylate (MMA) dental resin system. Degree of conversion (DC), flexural strength (FS) and modulus (FM), water sorption (WS) and solubility (WSL), antibacterial activity, and radio-opacity...

  20. Shear bond strength and SEM morphology evaluation of different dental adhesives to enamel prepared with ER:YAG laser.

    Science.gov (United States)

    Pires, Patrícia T; Ferreira, João C; Oliveira, Sofia A; Azevedo, Alvaro F; Dias, Walter R; Melo, Paulo R

    2013-01-01

    Early observations of enamel surfaces prepared by erbium lasers motivated clinicians to use laser as an alternative to chemical etching. Evaluate shear bond strength (SBS) values of different dental adhesives on Erbium:Yttrium Aluminum Garnet (Er:YAG) laser prepared enamel and to evaluate possible etching patterns correlations between dental adhesives and SBS values. One hundred bovine incisors were randomly assigned to SBS tests on enamel (n = 15) and to enamel morphology analysis (n = 5) after Er:YAG laser preparation as follows: Group I - 37% phosphoric acid (PA)+ ExciTE(®); Group II - ExciTE(®); Group III - AdheSE(®) self-etching; Group IV - FuturaBond(®) no-rinse. NR; Group V - Xeno(®) V. Teeth were treated with the adhesive systems and subjected to thermal cycling. SBS were performed in a universal testing machine at 5 mm/min. One-way ANOVA and post-hoc tests (P adhesive systems yielded significantly different SBSs. Acid etching significantly increased the adhesion in laser treated enamel. No differences in SBS values were obtained between AdheSE(®) and ExciTE(®) without condition with PA. FuturaBond(®) NR and Xeno(®) V showed similar SBS, which was lower in comparison to the others adhesives. No correlation between enamel surface morphology and SBS values was observed, except when PA was used.

  1. Preparation and Evaluation of Microcapsule Containing Volatile Oil of Herba Schizonepetae by Emulsion Solvent Diffusion Method

    Institute of Scientific and Technical Information of China (English)

    张立国; 欧阳霄雯; 倪力军; 史万忠

    2014-01-01

    Microcapsules of volatile oil containing Herba Schizonepetae (VOHS) were prepared by emulsion solvent diffusion method to improve the drug loading and reduce the amount of pharmaceutical excipients. Orthogonal assay was applied to optimize the preparation condition of microcapsulation, and the results illustrated that the ratio of ethyl cellulose (EC) to VOHS influenced the property of VOHS microcapsule significantly. GC-MS analysis indicated that some volatile components with low concentration in VOHS were lost after microencapsulation. The microcapsules prepared with optimum condition had good fluidity, and the holes on the surface of the microcapsules contributed to the release of VOHS. The particles of the microcapsule conformed to a normal distribution with the diameter of 45-220 µm. In the simulated intestinal fluid containing 0.2% sodium dodecyl sulfate, pulegone in VOHS microcapsule showed a certain degree of slow release. Compared withβ-cyclodextrin method, the microencapsulation used in the present work could reduce the amount of excipients and increase the drug loading. It was beneficial to reduce the dose of Chinese medicines containing volatile oils.

  2. Evaluation of a Hypocrea jecorina Enzyme Preparation for Hydrolysis of Tifton 85 Bermudagrass

    Science.gov (United States)

    Ximenes, E. A.; Brandon, S. K.; Doran-Peterson, J.

    Tifton 85 bermudagrass, developed at the ARS-USDA in Tifton, GA, is grown on over ten million acres in the USA for hay and forage. Of the bermudagrass cultivars, Tifton 85 exhibits improved digestibility because the ratio of ether- to ester-linked phenolic acids has been lowered using traditional plant breeding techniques. A previously developed pressurized batch hot water (PBHW) method was used to treat Tifton 85 bermudagrass for enzymatic hydrolysis. Native grass (untreated) and PBHW-pretreated material were compared as substrates for fungal cultivation to produce enzymes. Cellulase activity, measured via the filter paper assay, was higher for fungi cultivated on PBHW-pretreated grass, whereas the other nine enzyme assays produced higher activities for the untreated grass. Ferulic acid and vanillin levels increased significantly for the enzyme preparations produced using PBHW-pretreated grass and the release of these phenolic compounds may have contributed to the observed reduction in enzyme activities. Culture supernatant from Tifton 85 bermudagrass-grown fungi were combined with two commercial enzyme preparations and the enzyme activity profiles are reported. The amount of reducing sugar liberated by the enzyme mixture from Hypocrea jecorina (after 192 h incubation with untreated bermudagrass) individually or in combination with feruloyl esterase was 72.1 and 84.8%, respectively, of the commercial cellulase preparation analyzed under the same conditions.

  3. Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks.

    Science.gov (United States)

    Ibrahim, Hazem Mohammed; Sayed, Rafik Hamed; Abdel-Aziz, Wafaa Ragab; Soliman, Rafik Tawfik

    2017-08-01

    The present work aimed to develop lateral flow immunochromatographic strip (ICS) test for detection of Salmonella Enteritidis (SE) specific antibodies in chicken sera. A rapid lateral flow immunochromatographic test (LFIT) has been developed, in which SE Group D antigen labeled with the gold chloride molecules laid on the conjugate pad. Staphylococcus aureus protein A was used as capture antibody at the test line (T) of a nitrocellulose (NC) membrane and anti-SE antigen-specific rabbit antibodies were used as capture antibody at the control line (C) of the NC strip in the lateral flow layout device. Using the developed LFIT, the minimal amount of SE-specific antibodies that can be detected in chicken serum sample was 1427 enzyme-linked immunosorbent assay (ELISA) unit/100 µl that was equal to 0.1 µg (Ab)/100 µl sample. 100 suspected serum samples collected from a poultry flock were tested with the prepared SE-LFIT kits and the locally prepared stained Salmonella antigen, and the results were compared with those obtained from examination of these samples with Salmonella Group D antibody ELISA kit as the gold standard test. The sensitivity, specificity, and accuracy of the prepared SE-LFIT antigen kits were 94.4%, 90%, and 94%, respectively, while those obtained with stained Salmonella antigen were 88.8%, 90%, and 89%, respectively. The developed test is a simple field rapid test of high sensitivity, specificity, and accuracy that can improve and facilitates rapid field surveillance of salmonellosis among chickens.

  4. Preparation, Characterization, and Biological Evaluation of Poly(Glutamic Acid-b-Polyphenylalanine Polymersomes

    Directory of Open Access Journals (Sweden)

    Evgenia Vlakh

    2016-06-01

    Full Text Available Different types of amphiphilic macromolecular structures have been developed within recent decades to prepare the polymer particles considered as drug delivery systems. In the present research the series of amphiphilic block-copolymers containing poly(glutamatic acid as hydrophilic, and polyphenylalanine as hydrophobic blocks was synthesized and characterized. Molecular weights for homo- and copolymers were determined by gel-permeation chromatography (GPC and amino acid analysis, respectively. The copolymers obtained were applied for preparation of polymer particles. The specific morphology of prepared polymerosomes was proved using transmission electron microscopy (TEM. The influence on particle size of polymer concentration and pH used for self-assembly, as well as on the length of hydrophobic and hydrophilic blocks of applied copolymers, was studied by dynamic light scattering (DLS. Depending on different experimental conditions, the formation of nanoparticles with sizes from 60 to 350 nm was observed. The surface of polymersomes was modified with model protein (enzyme. No loss in biocatalytic activity was detected. Additionally, the process of encapsulation of model dyes was developed and the possibility of intracellular delivery of the dye-loaded nanoparticles was proved. Thus, the nanoparticles discussed can be considered for the creation of modern drug delivery systems.

  5. Preparation, characterization and evaluation of breviscapine lipid emulsions coated with monooleate-PEG-COOH.

    Science.gov (United States)

    Xiong, Fei; Xiong, Chen; Yao, Juan; Chen, Xinmei; Gu, Ning

    2011-12-15

    Series of monooleate-modified PEG with active carboxylic terminus on the other end (MO-PEG-COOH) were used to modify the lipid emulsions surface to prepare a sterically stabilized lipid emulsions for carrying Traditional Chinese Medicine - breviscapine. Based on the research of relationship between polymer structure and prolonged circulation activity, we developed an optimized formulation and a technological method to prepare the sterile and stable MO-PEG(10,000)-COOH (Bre-LE-PEG(10,000)) coated breviscapine lipid emulsions (Bre-LE) for intravenous administration. Follow the optimum preparation, the average particle size, polydispersity index, zeta potential, Ke value and content of final product were determined to be (207.1±8.5)nm, 0.197±0.005, (-33.6±2.0)mV, (21.1±2.3)% and (95.0±1.8)% respectively (n=3). The characteristics, stability and safety of Bre-LE-PEG(10,000) were also studied with Bre-LE as a control. Increased plasma concentration by surface modification of the lipid emulsions may enhance the pharmacological activity of breviscapine to promote blood circulation.

  6. Propellant Grade Hydrazine in Mono/Bi-propellant Thrusters: Preparation and Performance Evaluation

    Directory of Open Access Journals (Sweden)

    S. Krishnamachary

    2015-03-01

    Full Text Available Propellant grade hydrazine was prepared with 64 per cent yield and 95.5 per cent purity. Purity of the propellant grade hydrazine was determined using wet chemical, gas chromatographic (GC and eudiometric methods. It was observed that the compositions containing blends of hydrazine-methyl alcohol-ammonium nitrate and hydrazine-methyl alcohol-ammonium perchlorate were not found to be frozen even after cooling to -65 °C for 30 minutes. Mono and bi-propellant thrusters were designed and developed to demonstrate the performance of prepared propellant grade hydrazine as a promising rocket fuel. Five static tests with 22 N thruster and one static test with 1 N thruster were performed successfully in mono-propellant mode. The hurdles of chamber pressure oscillations were overcome by compact packing of the catalyst. The desired decomposition and chamber pressure were achieved. One static test was performed successfully with 60 N bi-propellant thruster. The desired chamber pressure and thrust were achieved. The combustion was smooth and C* achieved was higher than that of UH-25, N2O4 combination. The performance of prepared propellant grade hydrazine shows it as a promising rocket fuels.Defence Science Journal, Vol. 65, No. 1, January 2015, pp.31-38, DOI:http://dx.doi.org/10.14429/dsj.65.7986

  7. Preparation and evaluation of directly compressible forms of mutual prodrugs of ibuprofen

    Directory of Open Access Journals (Sweden)

    Bhosale A

    2006-01-01

    Full Text Available Tablets of mutual prodrugs of ibuprofen, i.e, ′ibuprofen with paracetamol′ and ′ibuprofen with salicylamide,′ were prepared by direct compression method. The preformulation studies such as flow property, solid state stability at elevated temperatures, solid state stability under different humidity conditions, photolytic stability and compatibility studies of prodrugs with excipients were also performed to design and develop tablet formulations of prodrugs. Quality control tests and in vivo studies of prepared tablets of prodrugs were performed. The result of preformulation studies revealed that prodrugs have good flow property, good solid state stability at elevated temperatures and unstable under different humidity conditions. The photolytic stability study showed that prodrugs are quite stable to light; hence prodrugs are nonphotolytic. The compatibility study indicated that there was no incompatibility or interaction between prodrugs and excipients, which were tried. The prepared tablets of prodrugs were found to satisfy all quality control requirements of tablets mentioned in the Indian Pharmacopoeia. In vivo study of tablet formulations of prodrugs confirmed that they possessed the ability of parent drug, i.e., ibuprofen. In vivo study also showed better extent of bioavailability (indicated by AUC0-24 of tablet of prodrugs as compared to tablets of ibuprofen.

  8. Preparation and evaluation of microencapsulated fast melt tablets of ambroxol hydrochloride

    Directory of Open Access Journals (Sweden)

    Jacob S

    2009-01-01

    Full Text Available Natural resources in general and plant materials in particular are receiving more attention due to their safety as pharmaceutical excipients. Present work assessed the potential of a natural polysaccharide, pectin to mask the bitter taste of ambroxol hydrochloride, by microencapsulation technique, and its possibility to formulate as a fast disintegrating dosage form. Taste masking is an important developmental challenge in fast dissolving drug delivery system since it dissolves or disintegrates in the patient′s mouth in close proximity to the taste buds. The prepared microspheres by emulsion solvent evaporation technique possessed good sphericity, smooth surface morphology, uniform and narrow size distribution (10-90 μm, when analyzed by scanning electron microscopy, laser diffraction and optical microscopy. Method of preparation has influenced the particle size and drug loading efficiency. Drug-polymer compatibility was confirmed by Fourier transform infrared spectroscopy and thin layer chromatography. DSC and X-ray diffraction studies revealed that the drug was dispersed inside the microspheres in the form of an insoluble matrix. The formation of microspheres was affected by glass transition temperature of the polymer, surfactant, type of plasticizers, volume of internal phase, stirrer speed etc. Fast dissolving tablets were prepared by the modification of melt granulation technique. The resulting granules were found to melt fast at body temperature, have smooth mouth feel and good physical stability. This study demonstrated that pectin could be a right choice in developing patient favored formulations for bitter drugs and can be utilized in fast disintegrating dosage forms as well.

  9. Demographic implications of the New United States certificates.

    Science.gov (United States)

    Lunde, A S; Grove, R D

    1966-06-01

    To assist in developing uniform reporting of vital events among the fifty states, Puerto Rico, and the Virgin Islands, the United States government prepares standard certificates of birth, fetal death, death, marriage, and divorce. These model forms are revised, with the assistance of the states, approximately every ten years. Revisions are now being prepared by the National Center for Health Statistics which will become effective beginning January 1, 1968. Important new source material for demography will be introduced.Most changes will appear in the Standard Certificate of Live Birth and in the Standard Certificate of Fetal Death. An item on education of father and mother will provide detailed national data on education and fertility. The date of the last live birth to the mother and the date of the last fetal death will provide information on previous pregnancy outcome and on child-spacing. The recording of state file numbers for mates born alive and dead in the same delivery will make it easier to match live birth and fetal death certificates for the preparation of detailed tabulations on multiple births. Several new items related to maternal and child health have also been added. No significent changes were planned for the Standard Certificate of Death.The Standard Certificate of Marriage will include as new items the education of the bride and groom, the date on which the last marriage, if any, ended, and specification of the officiant as a religious or civil official. The Standard Certificate of Divorce or Annulment will obtain information on the education of husband and wife, the approximate date on which the couple separated, the mode of dissolution of the previous marriage, and the total number of living children. It is anticipated that most of the new items will be included in the certificates of all the states. The National Center for Health Statistics will provide detailed tabulations related to these items, beginning with data year 1968.Demographers

  10. 40 CFR 92.208 - Certification.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Certification. 92.208 Section 92.208... POLLUTION FROM LOCOMOTIVES AND LOCOMOTIVE ENGINES Certification Provisions § 92.208 Certification. (a) This... the application for certification, test reports and data acquired from a freshly...

  11. 21 CFR 80.31 - Certification.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Certification. 80.31 Section 80.31 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL COLOR ADDITIVE CERTIFICATION Certification Procedures § 80.31 Certification. (a) If the Commissioner determines, after...

  12. 16 CFR 306.6 - Certification.

    Science.gov (United States)

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Certification. 306.6 Section 306.6... RATINGS, CERTIFICATION AND POSTING Duties of Refiners, Importers and Producers § 306.6 Certification. In... half number equal to or less than the number determined by you. This letter of certification will...

  13. 40 CFR 91.208 - Certification.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Certification. 91.208 Section 91.208... Certification. (a) In the application for certification a manufacturer must: (1) Submit a statement that the engines for which certification is requested will not, to the best of the manufacturer's belief, cause...

  14. 40 CFR 94.208 - Certification.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Certification. 94.208 Section 94.208... EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Certification Provisions § 94.208 Certification. (a) If, after a review of the application for certification, test reports and data acquired from an engine...

  15. 40 CFR 92.306 - Certification.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Certification. 92.306 Section 92.306... POLLUTION FROM LOCOMOTIVES AND LOCOMOTIVE ENGINES Certification Averaging, Banking, and Trading Provisions § 92.306 Certification. (a) In the application for certification a manufacturer or remanufacturer...

  16. 19 CFR 10.180 - Certification.

    Science.gov (United States)

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Certification. 10.180 Section 10.180 Customs... Frozen Beef § 10.180 Certification. (a) The foreign official's meat-inspection certificate required by U... certification below when fresh, chilled, or frozen beef is to be entered under the provisions of...

  17. 40 CFR 89.209 - Certification.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Certification. 89.209 Section 89.209... Provisions § 89.209 Certification. (a) In the application for certification a manufacturer must: (1) Declare...) Submit a statement that the engines for which certification is requested will not, to the best of...

  18. 49 CFR 624.7 - Certification.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 7 2010-10-01 2010-10-01 false Certification. 624.7 Section 624.7 Transportation... TRANSPORTATION CLEAN FUELS GRANT PROGRAM § 624.7 Certification. The applicant must use the certification contained in the Annual Notice of Assurances and Certifications published in the Federal Register...

  19. 2010 Guide to National Board Certification

    Science.gov (United States)

    National Board for Professional Teaching Standards, 2010

    2010-01-01

    This "Guide to National Board Certification" introduces teachers to the assessment process and provides them with useful information as they apply for National Board Certification or certification renewal, or order the "Take One!"[R] professional development kit. By participating in the National Board Certification process, teachers are joining…

  20. 5 CFR 1639.24 - Certification.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Certification. 1639.24 Section 1639.24... Certification. (a) The Board will provide a certification to the paying agency in all cases in which: (1) The... to appear at a hearing. (b) The certification must be in writing and must include: (1) A...

  1. 7 CFR 1216.70 - Certification.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Certification. 1216.70 Section 1216.70 Agriculture... INFORMATION ORDER Peanut Promotion, Research, and Information Order Certification of Peanut Producer Organizations § 1216.70 Certification. (a) Organizations receiving certification from the Secretary will...

  2. 40 CFR 90.208 - Certification.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Certification. 90.208 Section 90.208... EMISSIONS FROM NONROAD SPARK-IGNITION ENGINES AT OR BELOW 19 KILOWATTS Certification Averaging, Banking, and Trading Provisions § 90.208 Certification. (a) In the application for certification a manufacturer...

  3. 26 CFR 1.43-3 - Certification

    Science.gov (United States)

    2010-04-01

    ... 26 Internal Revenue 1 2010-04-01 2010-04-01 true Certification 1.43-3 Section 1.43-3 Internal... § 1.43-3 Certification (a) Petroleum engineer's certification of a project—(1) In general. A petroleum... requirements of section 43(c)(2)(A). A petroleum engineer's certification must be submitted for each...

  4. 5 CFR 179.208 - Certification.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Certification. 179.208 Section 179.208... Salary Offset § 179.208 Certification. (a) OPM salary offset coordinator shall provide a certification to... certification must be in writing and must state: (1) That the employee owes the debt; (2) The amount and...

  5. 46 CFR 107.258 - Crane certification.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Crane certification. 107.258 Section 107.258 Shipping... CERTIFICATION Inspection and Certification § 107.258 Crane certification. (a) The Coast Guard may accept current... West 44th Street, New York, NY 10036, on the Internet at http://www.icgb.com. (b) Crane...

  6. 40 CFR 94.306 - Certification.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Certification. 94.306 Section 94.306... EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Certification Averaging, Banking, and Trading Provisions § 94.306 Certification. (a) In the application for certification a manufacturer must: (1) Declare...

  7. Analysis of the green certificate market; Ein analyse av den groene sertifikatmarknaden

    Energy Technology Data Exchange (ETDEWEB)

    Storeboe, Inger Oeydis

    2001-04-01

    This report studies the advantages and disadvantages of a separate financial market for the environmental advantages in the production of electricity from renewable energy sources. This market solution is evaluated against other financial systems used to promote the production of green electricity. By starting from a general equilibrium model for the green certificate market, the report discusses how the adaptation in the certificate market is influenced by changes in the market conditions. The certificate market is combined with a quota market for carbon dioxide, with and without international trade with electricity and certificate and market power in the production of electricity from renewable energy sources.

  8. Ergonomic evaluation of the preparation of cuttings and minicuttings for eucalyptus seedling production, with the use of scissors.

    Science.gov (United States)

    Cunha, Eduarda Gabriela Santos; de Souza, Amaury Paulo; Minette, Luciano José

    2012-01-01

    This study was conducted in a nursery for eucalyptus seedling production in a forest Company, located in the southern state of Bahia, Brazil. It aimed to evaluate the ergonomic conditions of the preparation of cuttings and mini-cuttings with scissors for Eucalyptus seedling production to increase well-being, satisfaction and safety and minimizing the occurrence of occupational diseases, also improving the process efficiency and final product quality. Environmental conditions, levels of noise, postures, RSI risk and physical workload were assessed. The environmental conditions complied with the recommended norms, except at certain times of day, when workers should make compensation breaks. The workers that collect minicuttings presented postures classified as normal by OWAS. On the other hand, the posture of workers dealing with cutting preparation requires corrections in a short run. The workers were subjected to moderate RSI risk due to the high degree of repeatability found. The physical workload was classified as mild, with no need for ergonomic interventions.

  9. Preparation and pharmaceutical evaluation of acetaminophen nano-fiber tablets: Application of a solvent-based electrospinning method for tableting.

    Science.gov (United States)

    Hamori, Mami; Nagano, Kana; Kakimoto, Sayaka; Naruhashi, Kazumasa; Kiriyama, Akiko; Nishimura, Asako; Shibata, Nobuhito

    2016-03-01

    In this study, we developed nano-fiber-based tablets with acetaminophen (AAP; LogPow=0.51) for controlled-release delivery systems and evaluated in vitro drug dissolution and in vivo pharmacokinetics in rats. Nano-fibers made from methacrylic acid copolymer S (MAC; EUDRAGIT S100) and containing AAP were prepared using a solvent-based electrospinning (ES) method. In vitro dissolution rate profiles of AAP showed tableting pressure-dependent decreases and pH-dependent increases. The results of tablet tracking by X-ray irradiation showed tablets based on MAC nano-fibers did not disintegrate in the upper intestinal lumen and had the properties of a long-term-acting tablet. In addition, the in vitro release profiles of AAP from nano-fiber tablets prepared by dissolving MAC with AAP (NFT), nano-fiber tablets prepared by adsorbing AAP to drug-free MAC nano-fibers (NFTadso), and tablets prepared by adsorbing half the amount of AAP to MAC nano-fibers containing the remaining amount of AAP (NFThalf) showed independent controlled-release aspects of AAP compared with physical mixture tablets (PMT). In vivo pharmacokinetic studies in rats after intraduodenal administration of 14 mg/rat AAP in NFT, NFTadso, and NFThalf demonstrated that all these tablets based on MAC nano-fibers showed sustained-release profiles compared with PMT, and showed ultra-sustained release properties for AAP. These new tablets based on MAC nano-fibers did not disintegrate in the intestine in the lower pH region, and the tablets could regulate the release of AAP in a pH-dependent manner. The ES method is a useful technique to prepare nano-fibers and showed promising results as an oral delivery system for sustained-release regulation. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  10. Evaluation of the Efficiency of Different Disruption Methods on Yeast Cell Wall Preparation for β-Glucan Isolation

    Directory of Open Access Journals (Sweden)

    Anna Bzducha-Wróbel

    2014-12-01

    Full Text Available Selected methods for yeast cell disruption were evaluated to establish their suitability for cell wall preparation in the process of β-glucan isolation. The effect of different disruption methods on contents of total saccharides, β-glucans and proteins in the produced cell walls preparations was analyzed. The degree of cell wall purification from intracellular components was established on the basis of the ratio of solubilised material. The investigated methods included: cell exposure to hot water (autoclaving, thermally-induced autolysis, homogenization in a bead mill, sonication and their combinations. Experimental systems were prepared in water (pH 5.0 and pH 7.0 and Tris-HCl buffer (pH 8.0. The Saccharomyces cerevisiae yeast cell wall preparations with the highest degree of cytosol component release and purification of β-glucans were produced by 30 min of cell homogenization with zirconium-glass beads (0.5 mm in diameter. This was confirmed by the highest ratio of solubilised material (approx. 64%–67%. The thus-produced preparations contained ca. 60% of total saccharides, 13%–14% of β(1,3/(1,6-glucans, and approx. 35% of crude proteins. Similar results were obtained after autolysis coupled with bead milling as well as with sonication, but the time required for these processes was more than 24 h. Homogenization in a bead mill could be valuable for general isolation procedures because allows one to eliminate the different autolytic activity of various yeast strains.

  11. Teacher Preparation Evaluation Program (TPEP). AACTE Leadership Training on PBTE. Abstract.

    Science.gov (United States)

    Western Kentucky Univ., Bowling Green.

    The background of this abstract is the involvement of Western Kentucky University in an evaluative study designed to determine the feasibility of implementing a model such as the one described in Sandefur's "An Illustrated Model for the Evaluation of Teacher Education Graduates." The purposes of the study are as follows: (a) to aid faculty and…

  12. Preparation, characterization and in-vivo evaluation of microemulsions containing tamoxifen citrate anti-cancer drug.

    Science.gov (United States)

    Dehghani, Faranak; Farhadian, Nafiseh; Golmohammadzadeh, Shiva; Biriaee, Amir; Ebrahimi, Mahmoud; Karimi, Mohammad

    2017-01-01

    The aim of this study was to prepare and characterize a new nanocarrier for oral delivery of tamoxifen citrate (TMC) as a lipophilic oral administrated drug. This drug has low oral bioavailability due to its low aqueous solubility. To enhance the solubility of this drug, the microemulsion system was applied in form of oil-in-water. Sesame oil and Tween 80 were used as drug solvent oil and surfactant, respectively. Two different formulations were prepared for this purpose. The first formulation contained edible glycerin as co-surfactant and the second formulation contained Span 80 as a mixed surfactant. The results of characterization showed that the mean droplet size of drug-free samples was in the range of 16.64-64.62nm with a PDI value of <0.5. In a period of 6months after the preparation of samples, no phase sedimentation was observed, which confirmed the high stability of samples. TMC with a mass ratio of 1% was loaded in the selected samples. No significant size enlargement and drug precipitation were observed 6months after drug loading. In addition, the drug release profile at experimental environments in buffers with pH=7.4 and 5.5 showed that in the first 24h, 85.79 and 100% of the drug were released through the first formulation and 76.63 and 66.42% through the second formulation, respectively. The in-vivo results in BALB/c female mice showed that taking microemulsion form of drug caused a significant reduction in the growth rate of cancerous tumor and weight loss of the mice compared to the consumption of commercial drug tablets. The results confirmed that the new formulation of TMC could be useful for breast cancer treatment.

  13. Sildenafil vaginal suppositories: preparation, characterization, in vitro and in vivo evaluation.

    Science.gov (United States)

    Shanmugam, Srinivasan; Kim, Young-Hun; Park, Jeong-Hee; Im, Ho Taek; Sohn, Young Taek; Kim, Kyeong Soo; Kim, Yong-Il; Yong, Chul Soon; Kim, Jong Oh; Choi, Han-Gon; Woo, Jong Soo

    2014-06-01

    The main objective was to investigate the in vitro release profile/kinetics, and in vivo plasma pharmacokinetics (PK) and organ biodistribution (BD) of the prepared sildenafil vaginal suppositories (SVS). Suppositories containing 25 mg of sildenafil were prepared by the cream melting technique using Witepsol H-15 as a suppository base. The suppositories were characterized for weight variation, content uniformity, hardness, disintegration time and crystallinity change. The in vitro dissolution in pH 4.5, and in vivo plasma PK and organ BD of sildenafil from SVS in female Sprague Dawley rats, were also investigated. The mean weight variation, content uniformity, hardness and disintegration time of the prepared SVS were 1.127 ± 0.020 g, 98.25 ± 2.50%, 2.5 ± 0.08 kg and 9 ± 1.0 min, respectively. The release of sildenafil from the SVS was more than 90% at 30 min, with a release kinetic of Hixson--Crowell model and non-Fickian diffusion (n = 0.464). The plasma PK study demonstrated a significantly lower Cmax (∼10 times) and AUC0-24 h (∼13 times) of sildenafil in plasma following intravaginal (IVG) administration of suppositories compared to oral (PO) administration of sildenafil solution. Nevertheless, the organ BD study showed a phenomenally higher Cmax (∼40 times) and AUC0-24 h (∼20 times) of sildenafil in uterus following IVG administration of suppositories than PO administration of sildenafil solution. This study demonstrated enhanced sildenafil exposure in the uterus following IVG administration of SVS, which could be used to target the uterus for therapeutic benefits.

  14. Evaluation of specimen preparation techniques for micro-PIXE localisation of elements in hyperaccumulating plants

    Energy Technology Data Exchange (ETDEWEB)

    Kachenko, Anthony G. [Faculty of Agriculture, Food and Natural Resources, University of Sydney, New South Wales 2006 (Australia)], E-mail: a.kachenko@usyd.edu.au; Siegele, Rainer; Bhatia, Naveen P. [Institute for Environmental Research, Australian Nuclear Science and Technology Organization, Lucas Heights, New South Wales 2234 (Australia); Singh, Balwant [Faculty of Agriculture, Food and Natural Resources, University of Sydney, New South Wales 2006 (Australia); Ionescu, Mihail [Institute for Environmental Research, Australian Nuclear Science and Technology Organization, Lucas Heights, New South Wales 2234 (Australia)

    2008-04-15

    Hybanthus floribundus subsp. floribundus, a rare Australian Ni-hyperaccumulating shrub and Pityrogramma calomelanos var. austroamericana, an Australian naturalized As-hyperaccumulating fern are promising species for use in phytoremediation of contaminated sites. Micro-proton-induced X-ray emission ({mu}-PIXE) spectroscopy was used to map the elemental distribution of the accumulated metal(loid)s, Ca and K in leaf or pinnule tissues of the two plant species. Samples were prepared by two contrasting specimen preparation techniques: freeze-substitution in tetrahydrofuran (THF) and freeze-drying. The specimens were analysed to compare the suitability of each technique in preserving (i) the spatial elemental distribution and (ii) the tissue structure of the specimens. Further, the {mu}-PIXE results were compared with concentration of elements in the bulk tissue obtained by ICP-AES analysis. In H. floribundus subsp. floribundus, {mu}-PIXE analysis revealed Ni, Ca and K concentrations in freeze-dried leaf tissues were at par with bulk tissue concentrations. Elemental distribution maps illustrated that Ni was preferentially localised in the adaxial epidermal tissues (1% DW) and least concentration was found in spongy mesophyll tissues (0.53% DW). Conversely, elemental distribution maps of THF freeze-substituted tissues indicated significantly lower Ni, Ca and K concentrations than freeze-dried specimens and bulk tissue concentrations. Moreover, Ni concentrations were uniform across the whole specimen and no localisation was observed. In P. calomelanos var. austroamericana freeze-dried pinnule tissues, {mu}-PIXE revealed statistically similar As, Ca and K concentrations as compared to bulk tissue concentrations. Elemental distribution maps showed that As localisation was relatively uniform across the whole specimen. Once again, THF freeze-substituted tissues revealed a significant loss of As compared to freeze-dried specimens and the concentrations obtained by bulk tissue

  15. [Evaluation of molecular weights of hyaluronate preparations by multi-angle laser light scattering].

    Science.gov (United States)

    Yomota, Chikako

    2003-01-01

    Hyaluronate (HA), a glycosaminoglycan polysaccharide, has been used for osteoarthritis, periartritis of the shoulder and rheumatoid arthritis by intraarticular administration, and in ophthalmic surgery such as anterior segment surgery, and eye lotion. In this study, the molecular weight (Mw) of HA preparations were estimated by size-exclusion chromatography (SEC) system consisted of a refractometer (RI) and a multi-angle laser light scattering (MALS). From the results, it has been clarified that a successful characterization of HA samples with Mw up to 2 - 3 x 10(6) g/mol was possible by multidetector system.

  16. Preparation and evaluation of hollow molecular imprinted polymer for adsorption of dibenzothiophene

    Energy Technology Data Exchange (ETDEWEB)

    Yang Wenming [School of Material Science and Engineering, Jiangsu University, Zhenjiang 212013 (China); Liu Lukuan; Zhou Wei [School of the Environment, Jiangsu University, Zhenjiang 212013 (China); Xu Wanzhen, E-mail: xwz09@ujs.edu.cn [School of the Environment, Jiangsu University, Zhenjiang 212013 (China); Zhou Zhiping [School of Material Science and Engineering, Jiangsu University, Zhenjiang 212013 (China); Huang Weihong [School of the Environment, Jiangsu University, Zhenjiang 212013 (China)

    2012-06-15

    Hollow molecular imprinted polymer (H-MIP) was prepared using dibenzothiophene as template molecule, 4-vinylpyridine as functional monomer and K{sub 2}Ti{sub 4}O{sub 9} as carrier by surface imprinting technique combined with removing carrier process. The polymer was characterized by means of nitrogen adsorption, Fourier transform infrared spectrometry and scanning electron microscopy. H-MIP was provided with larger surface areas and pore features. The adsorption process was spontaneous by thermodynamic analysis. The adsorption process followed pseudo-second-order model by kinetics analysis and Freundlich equation by isotherm analysis. The selectivity performance of H-MIP was favorable.

  17. Preparation and primary biological evaluation of novel nitrido-188Re complexes/lipiodol

    Institute of Scientific and Technical Information of China (English)

    WANG Guanquan; WEI Hongyuan; LUO Shunzhong; HE Jiaheng; YANG Yuqing; WANG Wenjin; XIONG Xiaoling

    2008-01-01

    Two new nitrido-188Re complexes were prepared by a modified method in high yield.These complexes were stable in vitro.The biodistribution in normal mice showed that these nitrido-188Re complexes could accumulate in liver and dissipate quickly from almost all organs.TAE was performed with the use of lipiodol solutions of two complexes to rabbit VX2 liver tumor models.SPECT images showed that the two lipiodol solutions could remain in tumor for about 9 h (188ReN-NEPTDD/lipiodol) and 12 h (188ReN-NEMMPTDD/Iipiodol),respectively.

  18. Preparation and in vitro evaluation of chitosan matrices cross-linked by formaldehyde vapors.

    Science.gov (United States)

    Rao, B S; Murthy, K V

    2000-10-01

    Rifampicin-chitosan matrices were prepared by a chemical cross-linking method to develop a sustained-release form. The effects of cross-linking agent (formaldehyde) on the drug release rate and release kinetics were investigated in this study. Moreover, the kinetics of rifampicin released from chitosan matrices exposed to formaldehyde vapors for predetermined time intervals were analyzed using Ritger and Peppas exponential equation. The in vitro release kinetics exhibited a non-Fickian transport model. Increasing the exposure time to formaldehyde vapors decreased the release rate of rifampicin from chitosan matrices as a result of formation of greater structural strength and tighter texture.

  19. Preparation and preclinical evaluation of {sup 211}At-labelled compounds for {alpha}-particle radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    Larsen, R.H.

    1994-12-31

    The interest for {alpha}-particle emitters in internal radiotherapy is increasing due to improved conjugation chemistry. Experimental work has concentrated on {sup 211}At and {sup 212}Bi since these to nuclides have radiochemical and physical properties suitable for medical application. In this report it is demonstrated that biologically active {sup 211}At-labelled compounds can be prepared within a relatively short time allowing utilization of this 7.2 h {alpha}-particle. It is further shown that {sup 211}At-TP-3 treatment of human osteosarcoma in vitro gives promising therapeutic ratios. 76 refs., 5 figs., 3 tabs.

  20. Preparation and In vitro- In vivo Evaluation of Different Transdermal Formulations of Betahistine Dihydrochloride

    OpenAIRE

    Şahbaz, Sevinç; Dortunç, Betül

    2015-01-01

    Objective: Betahistine has been used in the treatment of diseases accompanied by impaired peripheral circulation (e.g. Ménière’s syndrome) to reduce the frequency of episodes of vertigo and tinnitus. The drug has a short half-life and should be taken three times daily due to the rapid elimination. Its contraindication in patients with peptic ulcer history and the difficulty of frequently dosing requires administration ways other than the oral route. The aim of this study is to prepare and eva...

  1. Evaluation of specimen preparation techniques for micro-PIXE localisation of elements in hyperaccumulating plants

    Science.gov (United States)

    Kachenko, Anthony G.; Siegele, Rainer; Bhatia, Naveen P.; Singh, Balwant; Ionescu, Mihail

    2008-04-01

    Hybanthus floribundus subsp. floribundus, a rare Australian Ni-hyperaccumulating shrub and Pityrogramma calomelanos var. austroamericana, an Australian naturalized As-hyperaccumulating fern are promising species for use in phytoremediation of contaminated sites. Micro-proton-induced X-ray emission (μ-PIXE) spectroscopy was used to map the elemental distribution of the accumulated metal(loid)s, Ca and K in leaf or pinnule tissues of the two plant species. Samples were prepared by two contrasting specimen preparation techniques: freeze-substitution in tetrahydrofuran (THF) and freeze-drying. The specimens were analysed to compare the suitability of each technique in preserving (i) the spatial elemental distribution and (ii) the tissue structure of the specimens. Further, the μ-PIXE results were compared with concentration of elements in the bulk tissue obtained by ICP-AES analysis. In H. floribundus subsp. floribundus, μ-PIXE analysis revealed Ni, Ca and K concentrations in freeze-dried leaf tissues were at par with bulk tissue concentrations. Elemental distribution maps illustrated that Ni was preferentially localised in the adaxial epidermal tissues (1% DW) and least concentration was found in spongy mesophyll tissues (0.53% DW). Conversely, elemental distribution maps of THF freeze-substituted tissues indicated significantly lower Ni, Ca and K concentrations than freeze-dried specimens and bulk tissue concentrations. Moreover, Ni concentrations were uniform across the whole specimen and no localisation was observed. In P. calomelanos var. austroamericana freeze-dried pinnule tissues, μ-PIXE revealed statistically similar As, Ca and K concentrations as compared to bulk tissue concentrations. Elemental distribution maps showed that As localisation was relatively uniform across the whole specimen. Once again, THF freeze-substituted tissues revealed a significant loss of As compared to freeze-dried specimens and the concentrations obtained by bulk tissue analysis

  2. Preparation and Evaluation of Contact Lenses Embedded with Polycaprolactone-Based Nanoparticles for Ocular Drug Delivery.

    Science.gov (United States)

    Nasr, Farzaneh Hashemi; Khoee, Sepideh; Dehghan, Mohammad Mehdi; Chaleshtori, Sirous Sadeghian; Shafiee, Abbas

    2016-02-08

    To improve the efficiency of topical ocular drug administration, we focused on development of a nanoparticles loaded contact lens to deliver the hydrophobic drug over a prolonged period of time. The cross-linked nanoparticles based on PCL (poly ε-caprolactone), 2-hydroxy ethyl methacrylate (HEMA), and poly ethylene glycol diacrylate (PEG-DA) were prepared by surfactant-free miniemulsion polymerization. The lens material was prepared through photopolymerization of HEMA and N-vinylpyrrolidone (NVP) using PEG-DA as the cross-linker. Effects of nanoparticles loading on critical contact lens properties such as transparency, water content, modulus and ion and oxygen permeabilities were studied. Nanoparticles and hydrogel showed high viability, indicating the absence of cytotoxicity and stimulatory effect. Drug release studies revealed that the hydrogel embedded with nanoparticles released the drug for a period of 12 days. The results of this study provide evidence that nanoparticles loaded hydrogels could be used for extended delivery of loteprednol etabonate and perhaps other drugs.

  3. Clozapine-carboxylic acid plasticized co-amorphous dispersions: Preparation, characterization and solution stability evaluation

    Directory of Open Access Journals (Sweden)

    Ali Ahmed Mahmoud Abdelhaleem

    2015-06-01

    Full Text Available This study addressed the possibility of forming of co-amorphous systems between clozapine (CZ and various carboxylic acid plasticizers (CAPs. The aim was to improve the solubility and oral bioavailability of clozapine. Co-amorphous dispersions were prepared using modified solvent evaporation methodology at drug/plasticizer stoichiometric ratios of 1:1, 1:1.5 and 1:2. Solid state characterization was performed using differential scanning calorimetry, X-ray diffraction and infra red spectroscopy. Highly soluble homogeneous co-amorphous dispersions were formed between clozapine and CAPs via hydrogen bonding. The co-amorphous dispersions formed with tartaric acid (1:2 showed the highest dissolution percentage (> 95 % in 20 minutes compared to pure crystalline CZ (56 %. Highly stable solutions were obtained from co-amorphous CZ-citric and CZ-tartaric acid at 1:1.5 molar ratio. The prepared dispersions suggest the possibility of peroral or sublingual administration of highly soluble clozapine at a reduced dose with the great chance to bypass the first pass metabolism.

  4. Enteric-coated sustained-release nanoparticles by coaxial electrospray: preparation, characterization, and in vitro evaluation

    Science.gov (United States)

    Hao, Shilei; Wang, Bochu; Wang, Yazhou; Xu, Yingqian

    2014-02-01

    Enteric-coated formulations can delay the release of drugs until they have passed through the stomach. However, high concentration of drugs caused by rapidly released in the small intestine leads to the intestinal damage, and frequent administration would increase the probability of missing medication and reduce the patient compliance. To solve the above-mentioned problems, aspirin-loaded enteric-coated sustained-release nanoparticles with core-shell structure were prepared via one-step method using coaxial electrospray in this study. Eudragit L100-55 as pH-sensitive polymer and Eudragit RS as sustained-release polymer were used for the outer coating and inner core of the nanoparticles, respectively. The maximum loading capacity of nanoparticles was 23.66 % by changing the flow rate ratio of outer/inner solutions, and the entrapment efficiency was nearly 100 %. Nanoparticles with core-shell structure were observed via fluorescence microscope and transmission electron microscope. And pH-sensitive and sustained drug release profiles were observed in the media with different pH values (1.2 and 6.8). In addition, mild cytotoxicity in vitro was detected, and the nanoparticles could be taken up by Caco-2 cells within 1.0 h in cellular uptake study. These results indicate that prepared enteric-coated sustained-release nanoparticles would be a more safety and effective carrier for oral drug delivery.

  5. Nano-Ammonium Perchlorate: Preparation, Characterization, and Evaluation in Composite Propellant Formulation

    Science.gov (United States)

    Kumari, A.; Mehilal; Jain, S.; Jain, M. K.; Bhattacharya, B.

    2013-07-01

    Nanomaterials are finding applications in explosives and propellant formulations due to their large surface area and high surface energy. This high surface energy is responsible for the low activation energy and increase in burning rate of the composition. Therefore, a successful attempt has been made to prepare nano-ammonium perchlorate using a nonaqueous method by dissolving ammonium perchlorate (AP) in methanol followed by adding the dissolved AP to the hydroxyl-terminated polybutadiene (HTPB), homogenization, and vacuum distillation of the solvent. The nano-AP thus formed was characterized using a NANOPHOX particle size analyzer (Sympatec, Germany), transmission electron microscopy (FEI, Hillsboro, OR), X-ray diffraction (PANalytical B.V., The Netherlands) and scanning electron microscopy (Ikon Analytical Equipment Pvt. Ltd., Mumbai, India) for particle size, purity, and morphology, respectively. The thermal behavior of nano-AP was also studied using differential thermal analysis-thermo gravimetric analysis (DTA-TGA). The data indicated that the particle size of the prepared AP was in the range of 21-52 nm and the thermal decomposition temperature was lower than that of coarse AP. Characterized nano-AP was subsequently used in composite propellant formulation up to 5% with 86% solid loading and studied for different properties. The results showed a 14% increase in burning rate in comparison to standard propellant composition with desired mechanical properties.

  6. Evaluation and additional recommendations for preparing a whole blood control material

    Directory of Open Access Journals (Sweden)

    Fink Nilda E.

    1998-01-01

    Full Text Available OBJECTIVE: The assessment of an easy to prepare and low cost control material for Hematology, available for manual and automated methods. MATERIAL AND METHOD: Aliquots of stabilized whole blood were prepared by partial fixation with aldehydes; the stability at different temperatures (4. 20 and 37 °C during periods of up to 8-9 weeks and aliquot variability with both methods were controlled. RESULTS: Aliquot variability with automated methods at day 1, expressed as CV% (coefficient of variation was: white blood cells (WBC 2.7, red blood cells (RBC 0.7, hemoglobin (Hb 0.6, hematocrit (Hct 0.7, mean cell volume (MCV 0.3, mean cell hemoglobin (MCH 0.6, mean cell hemoglobin concentration (MCHC 0.7, and platelets (PLT 4.6. The CV (coefficient of variation percentages obtained with manual methods in one of the batches were: WBC 23, Hct 2.8, Hb 4.5, MCHC 5.9, PLT 41. Samples stored at 4ºC and 20ºC showed good stability, only a very low initial hemolysis being observed, whereas those stored at 37ºC deteriobed a rapidly (metahemoglobin formation, aggregation of WBC and platelets, as well as alteration of erythrocyte indexes. CONCLUSIONS: It was confirmed that, as long as there is no exposure to high temperatures during distribution, this material is stable, allowing assessment, both esternal and internal, for control purposes, with acceptable reproductivity, both for manual and auttomatic methods.

  7. In vitro evaluation of novel mucoadhesive buccal tablet of oxytocin prepared with Diospyros peregrina fruit mucilages.

    Science.gov (United States)

    Metia, Pulak Kumar; Bandyopadhyay, Amal Kumar

    2008-04-01

    Novel mucoadhesive buccal tablets (NMBTs) of oxytocin were prepared as cores in cup fashion to release and permeate the drug unidirectionally toward the buccal mucosa to reach the systemic circulation directly. Adhesive cups for NMBTs were prepared with mucilage (DPM) isolated from edible Diospyros peregrina fruit. Mucoadhesive properties like shear and tensile and peel strengths of the adhesive cups were estimated on freshly excised bovine buccal mucosa. Core tablets were formulated with oxytocin using two penetration enhancers, sodium taurocholate and sodium thioglycollate. In vitro permeability studies of NMBTs were conducted in a Franz diffusion cell containing 50 ml of phosphate buffer, pH 6.6, at 37+/-0.2 degrees C through excised bovine buccal mucosa, and the amount of drug permeated was estimated at 220 nm on reverse-phase HPLC using a BDS Hypersil C(8) column with acetonitrile and potassium dihydrogen orthophosphate buffer 0.05 M, pH 6.6, (20 : 80 v/v) as the mobile phase, at flow rate of 1.25 ml/m. The NMBTs containing 0.75% w/w sodium taurocholate showed 26% permeability without damaging the histology of the buccal mucosa. The results suggest that this formulation may be a suitable alternative to oxytocin injections.

  8. Preparation and preliminary biological evaluation of 99Tcm-TADP as bone imaging agent

    Institute of Scientific and Technical Information of China (English)

    YAN Xiaohong; LUO Shineng; NIU Guosai; YE Wanzhong; YANG Min; WANG Hongyong; XIA Yongmei

    2008-01-01

    TADP, 2-(1H-1,2,4-triazol-1-yl)-1-hydroxyethane-1,1-diphosphonic acid, was synthesized by three step reactions from the raw material 1H-1,2,4-triazole. 99Tcm-TADP was prepared with 5 mg TADP at Ph 7.0 by joining 99TcmO4 with SnCl2·2H2O in aqueous solution for 10 min at room temperature. Both labeling yield and radiochemical purity of 99Tcm-TADP were more than 95%. The biodistribution in rats and bone scan in rabbits were also studied. The uptake of organ was expressed as %ID/g. The results showed that the bone uptake is up to 17.17%ID/g which is the maximum of bone uptake at 30 min after injection of 99Tcm-TADP in rats, bone-to-muscle and bone-to-blood uptake ratios were 61.32 and 13.21, respectively. The clear bone image of rabbit was obtained at 120 min after injection of 99Tcm-TADP and clearance in soft tissue was visible. The preparation of 99Tcm-TADP was convenient and 99Tcm-TADP exhibited high uptake in bone, and it would be a potential new bone imaging agent.

  9. Preparation and evaluation of bonded linear polymethacrylate stationary phases for open tubular capillary electrokinetic chromatography

    Energy Technology Data Exchange (ETDEWEB)

    Tan, Z.J.; Remcho, V.T. [West Virginia Univ., Morgantown, WV (United States)

    1997-02-15

    A new procedure for the preparation of thick polymethacrylate films bonded in 25 {mu}m i.d. fused-silica capillaries is developed. The etched silica surface is first modified with an unsaturated organosilane, which is later incorporated into the polymer film. The capillary is then filled with a monomer solution, and polymerization is initiated by incubation at elevated temperature. This thermoinitiation method enables the use of ordinary polyimide-jacketed capillaries in preparing the columns. The effect of monomer concentration on the resulting polymer film was studied by open tubular capillary electrokinetic chromatography using p-hydroxybenzoates (parabens) as test solutes. Good separations were achieved using short capillaries. Run-to-run retention time reproducibility was excellent, with RSDs of 2% (n = 50) being representative. For the linear polymer films produced, retention of analytes increased as the monomer concentration increased to a certain value, at which point the capacity factors level off with further increases in monomer concentration. The electroosmotic flow velocity decreases with increasing monomer concentration. The efficiency for an unretained test probe (acetone) reaches 270 000 plates/m. 13 refs., 10 figs., 1 tab.

  10. Performance Evaluation of Fast Microfluidic Thermal Lysis of Bacteria for Diagnostic Sample Preparation

    Directory of Open Access Journals (Sweden)

    Evangelyn C. Alocilja

    2013-01-01

    Full Text Available Development of new diagnostic platforms that incorporate lab-on-a-chip technologies for portable assays is driving the need for rapid, simple, low cost methods to prepare samples for downstream processing or detection. An important component of the sample preparation process is cell lysis. In this work, a simple microfluidic thermal lysis device is used to quickly release intracellular nucleic acids and proteins without the need for additional reagents or beads used in traditional chemical or mechanical methods (e.g., chaotropic salts or bead beating. On-chip lysis is demonstrated in a multi-turn serpentine microchannel with external temperature control via an attached resistive heater. Lysis was confirmed for Escherichia coli by fluorescent viability assay, release of ATP measured with bioluminescent assay, release of DNA measured by fluorometry and qPCR, as well as bacterial culture. Results comparable to standard lysis techniques were achievable at temperatures greater than 65 °C and heating durations between 1 and 60 s.

  11. Evaluation of two human plasma pools as candidate international standard preparations for syphilitic antibodies.

    Science.gov (United States)

    Rigsby, Peter; Ison, Catherine; Brierley, Matthew; Ballard, Ron; Hagedorn, Hans-Jochen; Lewis, David A; Notermans, Daan W; Riis, Jørn; Robertson, Peter; Seppälä, Ilkka J T; Rijpkema, Sjoerd

    2009-08-01

    A collaborative study was designed to asses two freeze-dried human plasma preparations containing anti-Treponema pallidum antibodies, 05/132 and 05/122, for their suitability as international reference reagents for syphilis serology. Both preparations are intended as replacements of the first international standard (IS) for syphilitic serum antibodies (HS). Samples were tested by eight laboratories using the T. pallidum passive particle agglutination assay (TPPA), the venereal disease research laboratory test (VDRL) and the rapid plasma reagin test (RPR). In addition a range of immunoassays was also used. The outcome of the collaborative study revealed that candidate standard 05/132 contains T. pallidum-specific IgG and IgM and is reactive in VDRL or RPR, and that 05/122 contains T. pallidum-specific IgG but is not reactive in either the VDRL or RPR test. Both 05/132 and 05/122 are reactive in the TPPA. On the basis of these results the Expert Committee on Biological Standardization of the World Health Organization designated 05/132 as the 1st IS for human syphilitic plasma IgG and IgM with a unitage of 3 IU per ampoule relative to HS and 05/122 as the 1st IS for human syphilitic plasma IgG with a unitage of 300 mIU per ampoule relative to 05/132.

  12. Evaluation of Topical Preparations Containing Curcuma, Acacia and Lupinus Extracts as an Anti-inflammatory Drugs

    Directory of Open Access Journals (Sweden)

    M M Hamzah

    2011-06-01

    Full Text Available Summary: This work was suggested on the basis of presence of curcuminoids in curcuma and the presence of flavonoidal constituent in acacia and lupinus. The aim of this study was to study their possible anti-inflammatory effect by separately formulation of the three extracts in a suitable gel formula for topical administration and comparison of the prepared gels with a standard gel in the market (diclosal Emulgel by using the carrageenan induced paw edema model in albino rats. The extracts were subjected to phytochemical screening tests using reported methods to determine the presence of various phytoconstituents. Gel formulation was prepared containing 8% of each extract separately in gel base, namely sodium carboxy methyl cellulose (NaCMC. The pharmacological screening revealed that percent reduction of edema produced by curcuma extract was 30.0%, by acacia extract was 4%, by ethanol fraction lupinus was 18% and by chloroform fraction lupinus was 11.3%, while diclofenac sodium topical gel produced 48% reduction of edema. Industrial relevance: Medicinal plants provide a host of chemical compounds, which have been optimized on the basis of their biological activities. Chemical compounds present in medicinal plants have shown great promise in the management of various inflammatory disorders and have continued to serve as alternative and complementary therapies. The present study will help the industry to produce herbal drug effective in the treatment of inflammation with less side effect and less costly when compared to the synthetic drugs.

  13. Evaluation and additional recommendations for preparing a whole blood control material

    Directory of Open Access Journals (Sweden)

    Nilda E. Fink

    1998-04-01

    Full Text Available OBJECTIVE: The assessment of an easy to prepare and low cost control material for Hematology, available for manual and automated methods. MATERIAL AND METHOD: Aliquots of stabilized whole blood were prepared by partial fixation with aldehydes; the stability at different temperatures (4. 20 and 37 °C during periods of up to 8-9 weeks and aliquot variability with both methods were controlled. RESULTS: Aliquot variability with automated methods at day 1, expressed as CV% (coefficient of variation was: white blood cells (WBC 2.7, red blood cells (RBC 0.7, hemoglobin (Hb 0.6, hematocrit (Hct 0.7, mean cell volume (MCV 0.3, mean cell hemoglobin (MCH 0.6, mean cell hemoglobin concentration (MCHC 0.7, and platelets (PLT 4.6. The CV (coefficient of variation percentages obtained with manual methods in one of the batches were: WBC 23, Hct 2.8, Hb 4.5, MCHC 5.9, PLT 41. Samples stored at 4ºC and 20ºC showed good stability, only a very low initial hemolysis being observed, whereas those stored at 37ºC deteriobed a rapidly (metahemoglobin formation, aggregation of WBC and platelets, as well as alteration of erythrocyte indexes. CONCLUSIONS: It was confirmed that, as long as there is no exposure to high temperatures during distribution, this material is stable, allowing assessment, both esternal and internal, for control purposes, with acceptable reproductivity, both for manual and auttomatic methods.

  14. Preparation and in-vitro evaluation of sustained-release metoclopramide hydrochloride microspheres.

    Science.gov (United States)

    Khidr, S H; Niazy, E M; el-Sayed, Y M

    1995-01-01

    Sustained-release metoclopramide microspheres were successfully prepared using cellulose propionate polymer at 1:2 drug to polymer ratio employing solvent evaporation technique and using acetone as the polymer solvent. The prepared microspheres at three stirring speeds were characterized with regard to their drug content, particle size distribution, surface topography using SEM and their release profiles at two different pHs at 37 degrees C. The surface of all samples was smooth with very few irregular elevations or depressions. The average particle size decreases as the rotational speed increases and was found to be 1320, 774 and 345 microns at 600, 900 and 1200 rpm, respectively. The average % drug entrapped was found to be 90.5, 100.1 and 60.0% at 600, 900 and 1200 rpm, respectively. Small differences in the release rate were observed due to different rotation speeds with an apparent lower dissolution for batches produced at 1200 rpm probably due to the properties of the coat. The effect of storage under accelerated conditions for 10 weeks on the release characteristics of these microspheres was also studied. The release properties of the microspheres did not change after storing them at 40 degrees C/80% relative humidity for 10 weeks.

  15. Preparation and evaluation of nattokinase-loaded self-double-emulsifying drug delivery system

    Directory of Open Access Journals (Sweden)

    Xiaona Wang

    2015-10-01

    Full Text Available In the present study, we prepared nattokinase-loaded self-double-emulsifying drug delivery system (SDEDDS and investigated its preliminary pharmacodynamics. The type and concentration of oil phase, inner aqueous phase and emulsifier were screened to prepare optimum nattokinase-loaded SDEDDS. Next, the optimum formulations were characterized based on microstructure, volume-weighted mean droplet size, self-emulsifying rate, yield, storage stability, in vitro release and in vivo pharmacodynamics studies. The water/oil/water multiple emulsions exhibited typical multiple structure, with relatively small volume-weighted mean droplet size 6.0 ± 0.7 μm and high self-emulsifying ability (self-emulsifying time <2 min. Encapsulation of nattokinase was up to 86.8 ± 8.2%. The cumulative release of nattokinase within 8 h was about 30%, exhibiting a sustained release effect. The pharmacodynamics study indicated that nattokinase-loaded SDEDDS could significantly prolong the whole blood clotting time in mouse and effectively improve the carrageenan-induced tail thrombosis compared with nattokinase solution. Moreover, we showed that SDEDDS could successfully self-emulsify into water/oil/water multiple emulsions upon dilution in dispersion medium with gentle stirring and effectively protect nattokinase activity in gastric environment. Our findings suggested that SDEDDS could be a promising strategy for peptide and protein drugs by oral administration.

  16. Double-loaded liposomes encapsulating Quercetin and Quercetin beta-cyclodextrin complexes: Preparation, characterization and evaluation

    Directory of Open Access Journals (Sweden)

    Jessy Shaji

    2012-01-01

    Full Text Available Beta-cyclodextrin (CD inclusion complexes of Quercetin were formed and characterized by Differential scanning calorimetry (DSC and Fourier transform infra-red spectroscopy (FTIR spectroscopy. Plain Quercetin liposomes using phosphatidylcholine and cholesterol were prepared and optimized. Factors such as ratio of lipids employed, drug:lipid ratio, etc. were fine tuned and optimized to achieve maximum entrapment of the Quercetin into the bilayer. Entrapment was further enhanced by double loading the liposomes. These were prepared by incorporating Quercetin as a plain drug as well as the inclusion complexes within the lipid bilayer and the aqueous compartment, respectively, of the liposomes using the thin film hydration technique. The highest entrapment was achieved with a lipid ratio of 9:1, and the amount of plain drug entering the bilayer was 1/10 th the amount of lipid employed. Double loading increased this value to one part of drug per five parts of lipid when Quercetin-beta-CD (1:1 mol/mol was entrapped. The release of Quercetin from liposomes was highest when the drug was entrapped in the form of a complex with beta cylodextrin. The high entrapment ability of Quercetin in the form of plain drug as well as beta cylodextrin-Quercetin complexes in comparison with plain drug is an indubitable advantage of this approach.

  17. Preparation and evaluation of double-phased mucoadhesive suppositories of lidocaine utilizing Carbopol and white beeswax.

    Science.gov (United States)

    Yahagi, R; Onishi, H; Machida, Y

    1999-08-27

    In an attempt to restrict drug absorption from suppositories to only the lower rectum, mucoadhesive lidocaine (LID) suppositories were prepared using Witepsol H-15 as a base, and Carbopol 934P (CP) and white beeswax (WAX) as additives. CP has a mucoadhesive property and WAX gives the suppositories stiffness. The suppositories containing 10% CP and 20% WAX stayed in the lower recta of rats for at least 2 h. Double-phased suppositories consisting of a front layer containing 10% CP and 20% WAX and a terminal layer containing LID and various amounts of CP were prepared. In vitro release profiles of LID from double-phased suppositories were similar to conventional single-phased suppositories containing CP alone. Values of AUC(0-6 h) and MRT of LID after administration of double-phased suppositories to rabbits were larger than those for single-phased suppositories with or without CP. On the other hand, the initial plasma metabolites concentrations after administration of double-phased suppositories were significantly lower and tended to exhibit delayed T(max) compared to single-phased suppositories. These results suggest that the double-phased mucoadhesive suppositories suppress initial metabolism of LID, and may be useful for improving bioavailabilities of drugs, like LID, which accept first-pass effect considerably.

  18. Preparation and evaluation of cobalt-nickel electrodes for hydrogen production by electrolysis

    Energy Technology Data Exchange (ETDEWEB)

    Fakeeha, A.H.; El Aleem, F.A. [King Saud Univ., Riyadh (Saudi Arabia). Dept. of Chemical Engineering

    2002-07-01

    Hydrogen can be produced by splitting water into hydrogen and oxygen through electrolysis. Different metals and combinations of metals have been screened in order to improve electrolysis. In addition, studies have been conducted to improve the methods of preparing electrodes that give lower potential. A cobalt-nickel electrode was used in this study for hydrogen production. The electro-kinetic parameters for such electrodes and its equivalent circuit were discussed. The combined nickel-cobalt electrode prepared by electro-codeposition exhibited significant catalytic activity towards hydrogen evolution from alkaline medium. The best performance was obtained for Ni-Co electrode in KoH solution at 70 degrees C. The electro-chemical impedance measurements and analysis resulted in an equivalent circuit of the present electrode in which a complex phase element was used to account for the complex rough surface of the electrode. The roughness factor of Ni-Co electrode is 31.9 at 70 degrees C. This is in good comparison with similar values of hydrogen evolution electrodes. 17 refs., 5 tabs., 10 figs.

  19. Preparation and evaluation of Ni-Zn electrode for electrolytic hydrogen production

    Energy Technology Data Exchange (ETDEWEB)

    Fakeeha, A.H.; Abdel-Aleem, F.A. [King Saud Univ., Riyadh (Saudi Arabia). College of Engineering Dept. of Chemical Engineering

    2001-06-01

    An important role in enhancing the economical and technical usage of water electrolysis process is the reduction of the over potential of hydrogen evolution reaction. For this study, the authors electroplated pure Nickel and co-deposited Nickel-Zinc with different loading, to prepare two groups of electrodes. A figure illustrated the over-potential for hydrogen evolution reaction of the prepared electrodes in 1 M KOH solution at 25 Celsius as function of current density. The Nickel-Zinc electrodes gave lower over-potential, as indicated by the Tafel slopes obtained. It was also revealed that as the amount of co-deposited material increased, the over-potential was reduced, within the experimental range. The equivalent circuit for the composite electrode was developed and the roughness factor was calculated from experimental data of alternating current impedance. The explanation for the superiority of the Zinc-Nickel electrode might reside in the high value obtained for the roughness factor. 17 refs., 3 tabs., 6 figs.

  20. RECENT DEVELOPMENTS IN HETEROGENEOUS IONEXCHANGE MEMBRANE: PREPARATION, MODIFICATION, CHARACTERIZATION AND PERFORMANCE EVALUATION

    Directory of Open Access Journals (Sweden)

    I. G. WENTEN

    2016-07-01

    Full Text Available Ion-exchange membranes (IEMs have been used in different processes and applications and have shown the advantages over other comparative processes. The extensive applications of IEMs in many areas have necessitated the improvement in their properties to achieve better performance and lower cost. As the first commercialized IEMs, heterogeneous membranes face the challenge to improve their lower selectivity and conductivity which associated to the higher heterogeneity. Recently, development of the heterogeneous IEMs is steadily growing which is driven by the need to obtain IEMs with better electrochemical properties, lower production cost, and easier preparation procedure. Additive blending, membrane surface modifications, and post-treatment are typical methods used to improve heterogeneous IEMs characteristics. The methods give the improvement in electro-chemical characteristics of heterogeneous membranes and provide the possibility to produce heterogeneous IEMs with comparable performance to homogeneous ones. In this paper, preparation, characterization, and performance of heterogenous IEMs are reviewed. In addition, recent progress on IEMs modifications is pointed out.

  1. A novel nanosuspension of andrographolide: Preparation, characterization and passive liver target evaluation in rats.

    Science.gov (United States)

    Guo, Ligang; Kang, Le; Liu, Xinrong; Lin, Xiangyun; Di, Donghua; Wu, Yong; Kong, Dexian; Deng, Yihui; Song, Yanzhi

    2017-03-14

    Andrographolide (Andro) is an excellent anti-inflammatory bicyclic diterpene γ-lactone. However, the poor solubility limits its application as injection for the treatment of acute inflammation. To meet the clinical needs for emergency, the Andro nanosuspensions injection was first prepared by the wet milling technique. The Andro nanosuspensions were composed of 3% Andro, 5% poloxamer 188 as the non-ionic stabilizer, 0.05% sodium deoxycholate or 0.1% sodium tauroursodeoxy cholate as the ionic stabilizer, and prepared by 350rpm speed and 12cycles of grinding with 0.4mm zirconium oxide pearls. The nanosuspensions showed hexagonal morphology with particle size of 300nm, and no change in crystalline state of Andro after milling. The nanosuspensions had a significant increase in saturation solubility, and could completely release within 0.25h (bulk Andro within 24h). The lyophilized product of Andro nanosuspensions with mannitol (5%) as lyoprotectant had good physical and chemical stability during the 6-month storage period. The pharmacokinetic and tissue distribution results showed that it was rapidly eliminated from the blood and largely distributed in the liver. Overall, the Andro nanosuspensions may be used as a potential formulation for the treatment of liver infections owing to its passive liver targeting function.

  2. Preparation and evaluation of suppositories for RK-28 (a new radiosensitizer) using rabbits

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Nak-Seo; Umejima, Hiroyuki; Miyata, Kaori; Okubo, Mayumi; Goto, Shigeru (Kyushu Univ., Fukuoka (Japan). Faculty of Pharmaceutical Sciences); Kato, Tetsuo; Mikami, Yukio; Sagitani, Hiromichi; Inoue, Haruyuki

    1994-05-01

    The pharmacokinetics of RK-28 [1-(4-hydroxy-2-butenoxy)methyl-2-nitroimidazole], a new hypoxic cell radiosensitizer, was studied following intravenous, oral, and rectal administration to rabbits. After an oral administration of RK-28 solution, the plasma concentration of RK-28 was considerably lower than that after intravenous administration through all time periods, and the absolute bioavailability was a mere 4.2%. It was presumed that a specific acid-catalyzed decomposition of RK-28 progressed in the stomach, and also, the absorbed RK-28 suffered first-pass effects in the liver. In contrast, the absolute bioavailability following the rectal administration of a solidified RK-28 suppository preparation was significantly increased in comparison with that obtained by other administration routes. Also, RK-28 emulsion suppositories were prepared by emulsifying various amounts of the drug with 1-hexadecanol and hydrogenated castor oil (HCO 60) at 80degC, and these were administered into the rabbit rectum. The resulting absolute bioavailability was 91% for the RK-28 emulsion suppository and 86.9% for an RK-28 emulsion suppository containing small amounts of Eudispert hv. These values were better than those in rats. The rectal administration of the RK-28 emulsion suppository containing small amounts of Eudispert hv showed a preferable plasma concentration-time pattern that reflects on radiation therapy. (author).

  3. Construction and evaluation of PVC conventional and tubular tripelennamine-selective electrodes: their application in pharmaceutical preparations.

    Science.gov (United States)

    Lima, J L; Montenegro, M C; Sales, M G

    1996-06-01

    The construction and evaluation of tripelennamine conventionally-shaped ion-selective electrodes and tubular detectors for the determination of this compound in pharmaceutical formulations are described. Electrodes with conventional configuration have been constructed without an internal reference solution, using several types of immobilized ionic sensors in PVC. The different electrode membranes were prepared by using tripelennamine tetraphenylborate as ionic-exchanger, dissolved in 2-nitrophenyl octyl ether (type A), dibutylphthalate (type B) and bis-(2-ethylhexyl)sebacate (type C) as plasticizer solvents. The general working characteristics of the different types of conventional electrodes were evaluated in tripelennamine solutions, with adjusted ionic strength, showing a linear response in the concentration range of about 4 x 10(-5) - 1 x 10(-1) M and a slope near the theoretical value. The electrodes presented a fast response (> 20 s) and a high reproducibility (> or = 0.2 mV per day). The electrode selectivity in the presence of some interferents, such as sodium, potassium, lithium, ammonium, chlorpheniramine, diphenydramine, promethazine, meclizine and pentazocine, was good, particularly for those whose sensor membrane was prepared with tripelennamine tetraphenylborate dissolved in 2-nitrophenyl octyl ether (type A). Tubular detectors were also prepared using the same sensor membrane and were evaluated in a low-dispersion flow-injection manifold. Under these conditions the detectors presented response characteristics similar to those of the corresponding conventionally-shaped electrodes. The analysis of different pharmaceutical forms (creams, syrups and gels) gave good results with mean recoveries of 99.8-100.6% when the experiments were conducted by direct potentiometry and 99.9-100.4% where the same determinations were conducted by flow-injection analysis with tubular detectors.

  4. Composing Standards and Composing Teachers: The Problem of National Board Certification.

    Science.gov (United States)

    Burroughs, Robert

    2001-01-01

    Considers whether National Board for Professional Teaching Standards (NBPTS) certification is as much an evaluation of teachers writing about their teaching as it is of the teaching itself, exploring two NBPTS certificate candidates' experiences, analyzing problems inherent in NBPTS standards and writing difficulties in NBPTS portfolio entries,…

  5. EVALUATED NUCLEAR STRUCTURE DATA FILE -- A MANUAL FOR PREPARATION OF DATA SETS.

    Energy Technology Data Exchange (ETDEWEB)

    TULI, J.K.

    2001-02-01

    This manual describes the organization and structure of the Evaluated Nuclear Structure Data File (ENSDF). This computer-based file is maintained by the National Nuclear Data Center (NNDC) at Brookhaven National Laboratory for the international Nuclear Structure and Decay Data Network. For every mass number (presently, A {le} 293), the Evaluated Nuclear Structure Data File (ENSDF) contains evaluated structure information. For masses A {ge} 44, this information is published in the Nuclear Data Sheets; for A < 44, ENSDF is based on compilations published in the journal Nuclear Physics. The information in ENSDF is updated by mass chain or by nuclide with a varying cycle time dependent on the availability of new information.

  6. New Osseodensification Implant Site Preparation Method to Increase Bone Density in Low-Density Bone: In Vivo Evaluation in Sheep

    OpenAIRE

    Trisi, Paolo; Berardini, Marco; Falco, Antonello; Podaliri Vulpiani, Michele

    2016-01-01

    Purpose: The aim of this study was to evaluate a new surgical technique for implant site preparation that could allow to enhance bone density, ridge width, and implant secondary stability. Materials and Methods: The edges of the iliac crests of 2 sheep were exposed and ten 3.8 × 10-mm Dynamix implants (Cortex) were inserted in the left sides using the conventional drilling method (control group). Ten 5 × 10-mm Dynamix implants (Cortex) were inserted in the right sides (test group) using the o...

  7. 76 FR 69801 - Conductor Certification

    Science.gov (United States)

    2011-11-09

    ... definition of ``conductor'' is a fundamental element of the conductor certification regulation and FRA does... and 219. G. Vision and Hearing Acuity BLET/UTU commented that proposed Sec. 242.117(k) should...

  8. Dialysis technicians' perception of certification.

    Science.gov (United States)

    Williams, Helen F; Garbin, Margery

    2015-03-01

    The Nephrology Nursing Certification Commission initiated this research project to study the viewpoint of dialysis technicians regarding the value of certification. A national convenience sample was obtained using both paper-and-pencil and online forms of the survey instrument. Demographic characteristics were obtained concerning age, race, ethnicity, education, and future employment planning. Technicians' primary work settings, the roles they fill, and the types of certification they hold are described. Incentives offered by employers are considered to explore how they contribute to job satisfaction. Understanding the perceptions of technicians regarding the benefits of certification and the limitations of workplace incentives should enable employers to improve their recruitment and retention programs. Information obtained may offer a baseline for future observations of the characteristics of these significant and essential contributors to the nephrology workforce.

  9. Preparation and pharmaceutical evaluation of nicotinamide stick for eradication of Staphylococcus epidermidis

    OpenAIRE

    Mohammad Ali Shahtalebi; Rahim Bahrinajafi; Sima Nahavandi

    2014-01-01

    Background: Staphylococcus epidermidis is a part of the skin′s normal flora that can cause acne. This study was designed to evaluate the efficacy of nicotinamide as a stick in eradication of staphylococcus. Materials and Methods: For evaluating of Anti-microbial effect on S. epidermidis used well plate method. We chose five plates for nicotinamide and five for mupirocin. The zones of inhibition were measured and compared. Results: The results showed nicotinamide stick had anti-microbial effec...

  10. Preparation and pharmaceutical evaluation of nicotinamide stick for eradication of Staphylococcus epidermidis

    OpenAIRE

    Mohammad Ali Shahtalebi; Rahim Bahrinajafi; Sima Nahavandi

    2014-01-01

    Background: Staphylococcus epidermidis is a part of the skin's normal flora that can cause acne. This study was designed to evaluate the efficacy of nicotinamide as a stick in eradication of staphylococcus. Materials and Methods: For evaluating of Anti-microbial effect on S. epidermidis used well plate method. We chose five plates for nicotinamide and five for mupirocin. The zones of inhibition were measured and compared. Results: The results showed nicotinamide stick had anti-microbial effec...

  11. Quality evaluation of stiff porridges prepared from Irish potato (Solanum tuberosum) and pigeon pea (Cajanus cajan) starch blends.

    Science.gov (United States)

    Abu, Joseph Oneh; Enyinnaya, Chinma Chiemela; James, Samaila; Okeleke, Ezinne

    2012-06-01

    Quality attributes of stiff porridges prepared from Irish potato and pigeon pea starch blends were studied. Starches were extracted from Irish potato and pigeon pea using a wet extraction method. Various ratios of the starches were mixed and analyzed for chemical, functional and pasting properties. The starch blends were then prepared into stiff porridges for sensory evaluation using a 20-man sensory panel. Substitution of Irish potato starch with pigeon pea starch led to increases in protein (0.15 to 1.2%), fat (0.26 to 0.56%) and ash (0.30 to 0.69%) while the amylose content of the starch blends decreased (from 23.8 to 18.4%) respectively. Functional properties such as bulk density (0.75 to 0.60 g/cm(3)), water absorption capacity (3.1 to 2.6 g water/ g sample) and dispersibility (58.6 to 42.7%) decreased significantly (P stiff porridges were not adversely affected by pigeon pea starch inclusion. Therefore it should be possible to incorporate up to 50% of low digestible pigeon pea starch into Irish potato starch from legumes such as pigeon pea as alternatives to cassava starch in the preparation of stiff porridges. Such porridges made from Irish potato and legume starches could provide additional incentive for individuals requiring decreased and or slow starch digestibility such as diabetics.

  12. Novel diphenyl dimethyl bicarboxylate provesicular powders with enhanced hepatocurative activity: preparation, optimization, in vitro/in vivo evaluation.

    Science.gov (United States)

    Aburahma, Mona Hassan; Abdelbary, Ghada Ahmed

    2012-01-17

    Diphenyl dimethyl bicarboxylate (DDB) is a hepatocurative agent used for treatment of various liver diseases. However, DDB therapeutic effectiveness is restricted by its low oral bioavailability that arises from its poor solubility and dissolution. Aiming at surmounting the aforementioned restrictions, DDB provesicular dry powders exemplified by proniosomes and proliposomes were prepared using film-deposition technique employing sorbitol as a carrier. Upon dilution with water, the provesicular powders rapidly transformed into vesicular dispersions, either liposomes or niosomes, which were characterized regarding their percent encapsulation efficiency (EE%), vesicle size and distribution, morphology and in vitro drug release. The revealed optimal provesicular powder was exposed to solid state characterization, stability testing and in vivo performance evaluation. Results showed that provesicular powders with acceptable flowability can be prepared using a weight ratio of lipids mixture to sorbitol of 1:20. Proniosomal powder composed of Tween 80:cholesterol:stearylamine in molar ratio 7:3:0.5 loaded on sorbitol was selected as the optimal formulation as it showed the highest EE% and dissolution enhancement for DDB. The elevated levels of liver enzymes in hepatically injured Albino Wister rats were significantly reduced (Ppowder in comparison to free DDB. This improvement was confirmed histopathologically by minimizing the associated hepatic injury. Accordingly, proniosomes can be assertively considered as a promising stable precursor for immediate preparation of niosomal carrier for DDB with enhanced dissolution and hepatocurative activity.

  13. Chemical and structural evaluation of activated carbon prepared from jute sticks for Brilliant Green dye removal from aqueous solution.

    Science.gov (United States)

    Asadullah, Mohammad; Asaduzzaman, Mohammad; Kabir, Mohammad Shajahan; Mostofa, Mohammad Golam; Miyazawa, Tomohisa

    2010-02-15

    Activated carbons have been prepared from jute sticks by chemical activation using ZnCl(2) and physical activation using steam for the removal of Brilliant Green dye from aqueous solution. The activated carbons and charcoal prepared from jute sticks were characterized by evaluating the surface chemistry, structural features and surface morphology. The maximum BET surface area was obtained to be 2304 m(2)/g for chemical activated carbon (ACC) while it is 730 and 80 m(2)/g for steam activated carbon (ACS) and charcoal, respectively. The FT-IR spectra exhibited that the pyrolysis and steam activation of jute sticks resulted in the release of aliphatic and O-containing functional groups by thermal effect. However, the release of functional groups is the effect of chemical reaction in the ZnCl(2) activation process. A honeycomb-type carbon structure in ACC was formed as observed on SEM images. Although charcoal and ACC were prepared at 500 degrees C the ACC exhibited much lower Raman sensitivity due to the formation of condensed aromatic ring systems. Due to high surface area and high porous structure with abundance of functional groups, the ACC adsorbed dye molecules with much higher efficiency than those of ACS and charcoal.

  14. Preparation of sucrose fatty acid esters as food emulsifiers and evaluation of their surface active and emulsification properties.

    Directory of Open Access Journals (Sweden)

    Megahed, Mohamed G.

    1999-08-01

    Full Text Available A simple method for the preparation of sucrose esters, from fatty acids and the least expensive sucrose, has been conducted. Fatty acids namely lauric, palmitic and oleic were used in their preparation in the absence of any organic solvent. Reasonable yields amounting to 86.5%, 87.3% and 88.6% for sucrose esters of laurate, palmitate and oleate were obtained, respectively. The products were evaluated for their hydrophile-lipophile balance (HLB, surface and interfacial tension properties as well as emulsion stability. The results showed that these sucrose esters exhibit similar properties as those of the commercially prepared compounds.

    Se ha llevado a cabo un método simple para la preparación de ésteres de sacarosa a partir de ácidos grasos y sacarosa de bajo coste. Se han usado ácidos laurico, palmítico y oleico en su preparación, en ausencia de solventes orgánicos. Se obtuvieron rendimientos aceptables del 86.5%, 87.3% y 88.6% para los ásteres del láurico, palmítico y oleico respectivamente. En los productos se evaluaron sus balances hidrófilo-lipófilo (HLB, sus propiedades de tensión superficial e interfacial así como su estabilidad en emulsiones. Los resultados mostraron que estos esteres de sacarosa exhiben propiedades similares a las de los compuestos preparados comercialmente.

  15. Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: in vitro mineralization and biological evaluation

    Science.gov (United States)

    Castro, Filipa; Ribeiro, Viviana P.; Ferreira, António; Oliveira, Ana L.; Reis, Rui L.; Teixeira, José A.; Rocha, Fernando

    2016-07-01

    This work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physico-chemical characterization of the synthesized HAp suggests high surface reactivity namely because of its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower concentrations (30 and 50 μg ml-1), although its cellular uptake behavior. Therefore, the prepared HAp shows immense potential as biomedical material, as well as drug and gene delivery vehicle. The results are also very promising regarding further scaling up of the process, as the designed methodology allow for the preparation in a continuous mode of nanosized HAp with controlled physico-chemical properties.

  16. Norfloxacin-loaded collagen/chitosan scaffolds for skin reconstruction: Preparation, evaluation and in-vivo wound healing assessment.

    Science.gov (United States)

    Mahmoud, Azza A; Salama, Alaa H

    2016-02-15

    Biomaterial scaffolds are versatile tools as drug carrier for treatment of wounds. A series of norfloxacin-loaded scaffolds were synthesized for treatment of wounds by combining collagen with two different types of chitosan using freeze-drying technique. Subsequently, scaffolds were screened in terms of morphology, water absorption and retention capacity, biodegradation, ex-vivo bioadhesive strength, in-vitro drug release biological compatibility, X-ray diffractometry, differential scanning calorimetry as well as in-vivo evaluation. The results indicate that the scaffold mechanical strength is dependent on the type of used chitosan. The prepared scaffolds contained interconnected porous architecture. The scaffolds had high water uptake and retention capacity with extended biodegradation rate. Scaffolds prepared with chitosan HCl showed superior bioadhesive strength compared to those prepared with low molecular weight chitosan. All scaffolds showed almost 100% drug release within 24h. As identified by the terahertz pulsed imaging measurements, there is single scaffold area with the same concentration. After 28 days of wound dressing with selected norfoloxacin-loaded or unloaded collagen/chitosan scaffolds in Albino rats, it was found that the tissue regeneration time was fast compared to non-treated wounds. Furthermore, the drug-loaded scaffolds showed normal structure of an intact epidermal layer as well as the underlying dermis as revealed by histopathological studies. The obtained results suggest that the investigated norfloxacin-loaded collagen/chitosan scaffold is a potential candidate for skin regeneration application. Copyright © 2015 Elsevier B.V. All rights reserved.

  17. Preparation and biological evaluation of radiolabelled antibodies with selected carbohydrate modifications

    Energy Technology Data Exchange (ETDEWEB)

    Qi, P. (Alberta Univ., Edmonton, AB (Canada). Faculty of Pharmacy); Sykes, T.R.; Noujaim, A.A. (Alberta Univ., Edmonton, AB (Canada). Faculty of Pharmacy Biomira Inc., Edmonton, AB (Canada)); Koganty, R.R.; Selvaraj, S. (Biomira Inc., Edmonton, AB (Canada))

    1993-05-01

    Two carbohydrates, N-acetylgalactosamine (GalNAc) and galactose-[beta]-1,3-GalNAc have been attached to human IgG (hIgG) by a novel linking reagent, hexafluoroglutaric acid dimethyl ester. Fluorine-19 NMR signals were used for the determination of the conjugation ratio. A third carbohydrate, sialic acid, was conjugated via reductive amination and the conjugation ratio determined by a resorcinol assay. The biological behaviour of these radiodinated antibodies with carbohydrate modification in normal mice indicates an enhanced liver uptake at 15 min post-injection with an associated change in circulating blood levels occurs for the galactose-based hIgG preparations. However, no significant differences in the biodistribution were observed for the sialic acid conjugate. These studies confirm the potential of carbohydrate-antibody conjugation for modifying the behaviour of antibodies in immunoscintigraphy and radioimmunotherapy. (author).

  18. Preparation and evaluation of Levosalbutamol sulphate chitosan microsphere for the treatment of asthma

    Directory of Open Access Journals (Sweden)

    D Dinal Patel

    2012-01-01

    Full Text Available Mucoadhesive drug delivery systems are those that provide intimate contact of the drug with the mucosa for an extended period of time. In present work, mucoadhesive chitosan microspheres of Levosalbutamol sulphate were prepared by Spray drying method. Formulations were characterized for various physicochemical attributes size, encapsulation efficiency, swelling ability, in vitro release study and mucoadhesion study by rat ileum. Through these parameters we conclude that the batch B 2 was found to be best mainly by mucoadhesion study and in vitro drug release. Mucoadhesion was found to be increased with incresed concentration of polymer and visa versa in case of drug release. Batch B 3 had also similar results with that of Batch B 2 . That′s why here Batch B 2 was said to be the best batch with less polymeric content as compare to Batch B 3 .

  19. Preparation and in vitro evaluation of sustained release drug delivery system for verapamil HCL

    Directory of Open Access Journals (Sweden)

    Bhalekar M

    2007-01-01

    Full Text Available Verapamil HCl is a calcium channel blocker administered on thrice a day dosage regimen. In the present study resinates of verapamil HCl were formulated using Indion resins. Drug loading process was optimized with respect to drug:resin ratio, pH of loading solution, and particle size of resin. Resinates were characterized using XRPD. In vitro drug release rates from resinate was not adequately sustained. Hence resinates were incorporated in pellets using extrusion spheronization to achieve desired release pattern. Optimum drug loading was seen at pH of 3.5 in drug resin ratio of 1:1 and was seen to increase with temperature. XRPD studies revealed verapamil to be present in amorphous form in resinates. Drug release from resinates was complete in four hours. Resinates were pelletized using hydroxypropylmethylcellulose. Resinate of Indion 254 with 5% hydroxypropylmethylcellulose fulfilled USP criteria for extended release verapamil preparation.

  20. Preparation of two component Fibrin Glue and its clinical evaluation in skin grafts and flaps

    Directory of Open Access Journals (Sweden)

    Jain P

    2003-01-01

    Full Text Available Tissue adhesive is one of the alternative to conventional suturing and has some added advantages. Fibrin glue has been used in obtaining haemostasis following trauma to spleen and liver. It has also been used in repair of dural tear and bronchial fistula. Fibrin glue is a biological tissue adhesive based on the final stage of coagulation wherein. Thrombin acting on fibrinogen converts it into fibrin. Thus, it has two components, one is fibrinogen and another is thrombin. We have prepared both components of fibrin glue. Fibrinogen was obtained from patient's own blood and thrombin from fresh frozen plasma of screened healthy donor. The glue was used in 20 cases requiring skin graft or flap. The results were compared with conventional suturing method. Use of the fibrin glue is simple, safe, cost effective, and rapid technique to fix the skin grafts and flaps with avoidance of peroperative bleeding and postoperative collection. It also has better overall results.