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Sample records for prepara tu mochila

  1. O Problema da mochila compartimentada e aplicações

    Directory of Open Access Journals (Sweden)

    Fabiano do Prado Marques

    2002-07-01

    Full Text Available O Problema da Mochila Compartimentada é uma variação do clássico problema da mochila e pode ser enunciado considerando-se a seguinte situação hipotética: um alpinista deve carregar sua mochila compossíveis itens de seu interesse. A cada item atribui-se o seu peso e um valor de utilidade (até aqui, o problema coincide com o clássico Problema da Mochila. Entretanto, os itens são de agrupamentos distintos (alimentos, medicamentos, utensílios, etc. e devem estar em compartimentos separados na mochila. Os compartimentos da mochila são flexíveis e têm capacidades limitadas. A inclusão de um compartimento tem um custo fixo que depende do agrupamento com que foi preenchido, além de introduzir uma perda da capacidade da mochila. O problema consiste em determinar as capacidades adequadas de cada compartimento e como esses devem ser carregados, maximizando o valor de utilidade total, descontado o custo de incluir compartimentos. Neste trabalho propomos um modelo de otimização não linear inteiro para o problema e algumas heurísticas para sua resolução. Uma aplicação prática de relevância deste problema aparece no corte de bobinas de aço, sujeito à laminação, detalhado em apêndice.The Compartmentalized Knapsack Problem is a variation of the classical knapsack problem and can be stated considering the following hypothetical situation: an alpinist must load his knapsack with items. Two numbers, a weight and an utility value are associated with each item (so far, the problem coincides with the classical integer Knapsack Problem. However, the items are of different classes (food, medicine, utensil, etc. and they have to be packed into separate compartments inside the knapsack. The compartments are flexible and have limited capacities. Each compartment has a fixed cost that depends on the class of the items and, in addition, each new compartment introduces a loss on the capacity of the knapsack. The Compartmentalized Knapsack

  2. La mochila "rayá" : del símbolo a la subsistencia

    Directory of Open Access Journals (Sweden)

    Cristina Echavarría Usher

    1999-09-01

    Full Text Available La mochila "rayá", la mochila en la que cargan los campesinos de toda la llanura del Caribe colombiano el pedazo de queso bajero y la "miguita" de panela, el frasco de café y el bollo de maíz para desayunar en el monte; la mochila que usan también los estudiantes y obreros; esta mochila de fique con rayas de brillantes colores, es tejida por mujeres y niños artesanos que viven en los pueblos y veredas de la región de Atánquez, situada en el árido piedemonte del sureste de la Sierra Nevada de Santa Marta.

  3. Tu Women

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    When honored guests visit, Tu women will block the door and invite them to show their respect by drinking three cups of wine. Only after this ritual can the guests be led indoors and shown to their seats.

  4. Caracterização da postura estática de estudantes com sobrecarga (mochilas) às costas

    OpenAIRE

    Minhoto, Sílvia Maria Anciães

    2013-01-01

    O objectivo do presente estudo foi avaliar as alterações posturais ao suportar a mochila escolar na postura ortostática, em estudantes com idade entre os 10 e 12 anos, das turmas do 5.º e 6.º ano do Agrupamento de Escolas de Mogadouro – 2.º Ciclo. Participaram no estudo 65 estudantes (33 do sexo feminino e 32 do sexo masculino), devidamente autorizados que foram avaliados quanto aos parâmetros antropométricos (Estatura, Massa corporal, Massa da mochila e Índice da massa corporal) e avaliação ...

  5. Efeitos da sobrecarga das mochilas escolares nas alterações posturais e de equilíbrio

    OpenAIRE

    Jardim, Mónica Maria Rodrigues

    2013-01-01

    Mestrado em Fisioterapia Introdução: As cargas das mochilas escolares produzem mudanças na postura em pé, quando comparadas com a postura sem carga. Há pouca evidência quanto ao facto da exposição a carga posterior produzir efeitos prejudiciais no tecido ósseo. Objectivo: Analisar os efeitos da carga em mochilas escolares sobre a postura e o equilíbrio. Amostra: Por conveniência, constituída por 19 indivíduos saudáveis, de ambos os géneros, de duas turmas do 8º ano, com idades compreend...

  6. Algoritmo multiobjetivo NSGA-II aplicado al problema de la mochila

    OpenAIRE

    2008-01-01

    Este trabajo presenta la filosofía del algoritmo multiobjetivo elitista NSGA-II, explicando su esquema de funcionamiento y los mecanismos especiales que permiten la preservación y la evolución de soluciones Pareto-óptimas. Este algoritmo se aplica sobre el problema de optimización clásico correspondiente al problema de la mochila adaptado para optimización multiobjetivo. También se desarrolla una propuesta alternativa para ser comparada con el esquema básico del NSGA-II. This paper...

  7. Tu nos aposte protege

    DEFF Research Database (Denmark)

    Frederiksen, Britta Olrik

    2007-01-01

    Aspirationen af p, t og k i dansk er stedmoderligt behandlet af den gren af den historiske sprogforskning der bygger på skriftligt kildemateriale. Skrivemåden tu nos aposte protege for tu nos ab hoste protege 'bekyt du os mod fjenden' i en tilskrift fra anden halvdel af 1300-tallet i...

  8. A influência do peso da mochila escolar na variação angular do joelho

    OpenAIRE

    Noronha, Maria; Martins, Rodrigo; Alves, Sandra

    2014-01-01

    A marcha é uma atividade de extrema importância na funcionalidade e independência do indivíduo no seu dia-­‐a-­‐dia, de forma que qualquer fator interno ou externo que a modifique torna-­‐se importante de avaliar. Objetivo do estudo: Identificar as alterações angulares do joelho, decorrentes da variação do peso em mochilas escolares. Metodologia: ...

  9. The Pride of Tu Women

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    The Tu ethnic group, with a population of 191,624, live mainly in Huzhu Tu Autonomous County of Qinghai Province. The Tu people, who subsist on agriculture, believe in Lamaism. Tu women are quite fond of their traditional dress with the rainbow-colored sleeves. The black stands for the earth; green, the seedlings; yellow, the mature wheat; blue, the waters and red, the Sun.

  10. Associação entre a dor músculo-esquelética e o uso de mochilas em crianças de idade escolar

    OpenAIRE

    Moreira, Diogo; Festas, Clarinda; Lourenço, Manuel

    2010-01-01

    Pretendeu-se avaliar a presença de dor músculo-esquelética e a sua associação com o género, o ano escolar, o peso relativo e o tipo de transporte da mochila, e também averiguar a distribuição de dor, consoante os pesos relativos das mochilas (15%). Foram recolhidos 104 questionários, adequadamente preenchidos, com a identificação e as características antropométricas, a distribuição de dor num bodychart e as características da mochila. A amostra foi constituída por 54,8% de rapa...

  11. A influência do peso da mochila escolar de alças sobre o controle postural de crianças

    OpenAIRE

    Aryane Karoline Vital de Souza

    2016-01-01

    Introdução: A literatura recomenda que a carga das mochilas escolares esteja entre 10 e 15% da massa corporal da criança, para que se minimize a instalação de alterações posturais e mudanças na marcha. O controle postural é componente fundamental para o desenvolvimento de habilidades e funções na criança. No entanto, há escassez de estudos sobre o efeito da mochila escolar no controle postural em crianças, além disso, não são conclusivos. Objetivo: Investigar a influência do peso das mochilas...

  12. Análise cinética e cinemática da marcha durante o transporte da mochila escolar com rodas

    OpenAIRE

    Almeida, Pablo de

    2011-01-01

    Resumo: O objetivo deste estudo foi analisar e comparar as variáveis temporais/espaciais cinéticas e cinemáticas durante a marcha no plano e o transporte de mochilas com rodas (carga referente a 10%PC) em escolares de 7 a 10 anos. Dez crianças, saudáveis (7,8 ± 1,6 anos) foram selecionadas à participarem do estudo que envolveu duas condições experimentais. A primeira condição envolveu a marcha no plano, enquanto que na segunda condição ocorreu o transporte de mochila provida de rodas no plano...

  13. Tu proyecto de vida

    OpenAIRE

    Ramos, Georgina

    2012-01-01

    - Párrafo Inicial - Conociendo a Liliana N. - La intervención de la psicóloga Adriana - Tu proyecto de vida - Referencias - Notas de enseñanza o Resumen o Objetivos o Temas relacionados o Plan de aplicación para el aprendizaje o Análisis o Epílogo

  14. Tu proyecto de vida

    OpenAIRE

    Ramos, Georgina

    2012-01-01

    - Párrafo Inicial - Conociendo a Liliana N. - La intervención de la psicóloga Adriana - Tu proyecto de vida - Referencias - Notas de enseñanza o Resumen o Objetivos o Temas relacionados o Plan de aplicación para el aprendizaje o Análisis o Epílogo

  15. O efeito do uso de mochila na cinemática da marcha de crianças =The effect of backpack use in children’s gait kinematics

    OpenAIRE

    Flores, Felipe

    2006-01-01

    Objetivo: Este estudo teve como objetivo verificar diferenças cinemáticas na marcha de crianças com e sem o uso de mochila. Materiais e métodos: Foi analisada a marcha de 4 crianças, todas com 9 anos de idade, com e sem o uso de mochila, utilizando-se de equipamentos de análise biomecânica. O percentual de carga da mochila utilizada pelos sujeitos do estudo foi controlado e foram analisados os parâmetros de tempo dos passos, a velocidade da marcha e a variação angular do joelho. Resulta...

  16. Efeitos de sessões educativas no uso das mochilas escolares em estudantes do ensino fundamental I Effects of educational sessions on school backpack use among elementary school students

    OpenAIRE

    SMS Fernandes; RA Casarotto; SMA João

    2008-01-01

    OBJETIVO: Avaliar a modificação na quantidade de carga transportada, o modelo e o modo de transportar mochilas escolares após sessões educativas. MÉTODOS: Estudo de uma série de casos, com 99 crianças de sete a 11 anos do ensino fundamental, em escola particular da cidade São Paulo, São Paulo. Foram avaliadas a massa corporal (kg) e estatura dos alunos (cm), quantidade de carga transportada nas mochilas (kg). Os modelos e os modos de transporte das mochilas foram avaliados por filmagem pré e ...

  17. Os efeitos de diferentes pesos de mochila no alinhamento postural de crianças em idade escolar.

    Directory of Open Access Journals (Sweden)

    Lílian Gerdi Kittel Ries

    2013-01-01

    Full Text Available O objetivo deste estudo foi analisar o efeito imediato de diferentes pesos de mochila na postura corporal e avaliar a confiabilidade dessas medidas. Foram avaliados 50 escolares com idades entre oito e 14 anos. Os escolares foram fotografados no plano coronal anterior e sagital, com diferentes cargas relativas ao peso: sem mochila, com o peso habitualmente utilizado, com 10% do peso corporal (PC, 15 % do PC e 20% do PC. Para comparar os ângulos de assimetria da cabeça, de ombros, pélvica, do trocanter, dos joelhos, maleolar, corporal, sagital da cabeça, sagital dos ombros e sagital corporal foi utilizada a Análise de Variância para Medidas Repetidas e o Teste de Friedman. A confiabilidade foi analisada por meio do Coeficiente de Correlação Intraclasse (ICC. Foram observadas diferenças estatisticamente significativas no ângulo sagital cervical (p < .00, no ângulo sagital corporal (p < .00 e no ângulo sagital dos ombros (p < .01 com o aumento da carga. Também obteve-se alta confiabilidade inter-avaliador (ICC entre .83 a .99 para todos os ângulos avaliados nas diferentes posturas (p < .05. Este estudo não sustenta a recomendação de que o peso da mochila não deve exceder 10% do PC, já que cargas inferiores também ocasionaram anteriorização da cabeça e do corpo.

  18. Análise cinemática do perfil da coluna vertebral durante o transporte de mochila escolar

    OpenAIRE

    Carvalho, Luiz Antônio Penteado de

    2004-01-01

    Orientador: André Luiz Félix Rodacki Co-orientadora: Maria Lúcia Leite Ribeiro Okimoto Dissertaçao (mestrado) - Universidade Federal do Paraná, Setor de Tecnologia, Programa de Pós-Graduaçao em Engenharia Mecânica. Defesa: Curitiba, 2004 Inclui bibliografia Área de concentraçao: Gestao de produçao e ergonomia Resumo: O objetivo desta pesquisa foi determinar alterações no padrão cinemático postural em escolares durante o transporte de mochila dorsal de alça bilateral de ombros....

  19. Core TuLiP

    OpenAIRE

    Czenko, M.R.; Etalle, Sandro

    2007-01-01

    We propose CoreTuLiP - the core of a trust management language based on Logic Programming. CoreTuLiP is based on a subset of moded logic programming, but enjoys the features of TM languages such as RT; in particular clauses are issued by different authorities and stored in a distributed manner. We present a lookup and inference algorithm which we prove to be correct and complete w.r.t. the declarative semantics. CoreTuLiP enjoys uniform syntax and the well-established semantics and is express...

  20. Efeitos da orientação postural na utilização de mochilas escolares em estudantes do ensino fundamental

    OpenAIRE

    Susi Mary de Souza Fernandes

    2007-01-01

    OBJETIVO: Avaliar o efeito de sessões educativas no peso, modelo e modo de transportar mochila escolar. MÉTODO: Estudo com 99 crianças, de 7 a 11 anos cursando ensino fundamental em escola particular de São Paulo. Na avaliação foi medido peso do sujeito (kg) e da mochila (kg), tipo e modo de transporte por filmagem pré e pós intervenção. Como medida de intervenção, os sujeitos foram submetidos à sessão educativa que consistiu em orientações sobre coluna vertebral e vivência prática de postura...

  1. The Dress of Tu Women

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    Women of the Tu ethnicity, who live on the highlands in northwest China, wear bright and colorful dresses. The married women favor shirts with sleeves decorated in a rainbow of colored flowers: yellow, green, blue, red, purple, black

  2. O uso da mochila escolar e suas implicações posturais no aluno do Colégio Militara de Curitiba

    OpenAIRE

    Ferst, Nilton Cesar

    2003-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de Produção. A pesquisa tem por objetivo relacionar o uso da mochila escolar com o surgimento e/ou aumento de desvios posturais na coluna vertebral dos alunos do Colégio Militar de Curitiba, mais precisamente em 105 alunos da 5ª série do ensino fundamental. Com a fundamentação teórica embasada na anatomia da coluna vertebral; na análise ergonômica do posto de trabalh...

  3. El peso de la sabiduría: estudio y análisis del peso medio de las mochilas y sus repercusiones, en un instituto de Secundaria.

    OpenAIRE

    Suárez Gómez, Marcos; González Gutiérrez, Daniel; Martinez Pardo, Victor; García Fernández, Jessica; Buenaga Fernández, Ángel

    2005-01-01

    La razón por la que se eligió este tema (dolores de espalda, efectos, características, elementos influyentes...) por unanimidad fue como consecuencia de un hecho que le sucedió a uno de los miembros de nuestro grupo a principios del curso 2003/2004: este componente, sin el cual no habríamos hallado una excusa digna para realizar una buena investigación, carecía en las primeras semanas de curso de mochila ó, por llamarlo de otra manera de un transporte cómodo para su material escolar. Los libr...

  4. Transitando el camino de construcción de la lengua escrita, con La Mochila Mágica

    Directory of Open Access Journals (Sweden)

    Zulay Maldonado González

    2009-01-01

    Full Text Available La lectura y la escritura son procesos indispensables en la era del conocimiento y de la información en la que se encuentra la sociedad. Sin embargo, estudios, estadísticas e informes indican que existen gran cantidad de personas que no poseen estas competencias básicas para responder efectivamente a las exigencias personales, laborales y profesionales. Ante esta situación surge La Mochila Mágica como un programa de promoción de lectura, que busca despertar el placer y el amor por la lectura en los niños y niñas desde su más tierna edad. A través del préstamo circulante y actividades de actualización apoya a docentes, padres, madres y otros adultos significativos en el proceso de alfabetización inicial. Para determinar su efectividad, seleccionamos como escenario para su aplicación el Jardín de Infancia Congreso de Angostura, del Municipio San Cristóbal, Estado Táchira, Venezuela. Realizamos una investigación de corte cualitativo, la cual se sustentó en los aportes teóricos de la psicolingüística, la sociolingüística, la perspectiva histórica cultural de Vigotsky y las últimas investigaciones de la Neurociencia. Después de un año de intervención continuada y sistemática obtuvimos resultados significativos. Por ello se ha seguido implementado el programa a lo largo de 10 años, durante los cuales se ha tratado de crear las condiciones para que se desarrolle la conciencia social sobre la importancia de la lectura, como herramienta del pensamiento que abre los caminos hacia el ejercicio de la libertad, el conocimiento de sí mismo y el respeto por la vida.

  5. Uso da mochila em crianças pré-escolares : uma experiência de pesquisa-ação

    OpenAIRE

    Larissa Bertolini Andreatta

    2013-01-01

    Introdução: Os fatores mais destacados para as alterações posturais e dores na coluna vertebral em crianças referem-se aos hábitos relacionados às atividades escolares, tais como, a maneira de transportar o material escolar, o modelo e o peso da mochila. Quando hábitos posturais incorretos são adotados, desde a infância, são considerados motivo de grande preocupação, visto que as crianças estão em processo de formação do esqueleto e tornam-se mais suscetíveis a deformações e lesões nas estrut...

  6. O efeito do uso de mochila na cinemática da marcha de crianças =The effect of backpack use in children’s gait kinematics

    Directory of Open Access Journals (Sweden)

    Flores, Felipe

    2006-01-01

    Conclusão: Pode-se concluir que os resultados do presente estudo sugerem que a recomendação de que a massa das mochilas não ultrapasse o valor de 10% da massa corporal para crianças estudantes do ensino fundamental, parece confirmar-se como verdadeiro, pois não acarretou, de maneira geral, alterações significativas nos padrões cinemáticos testados neste estudo.

  7. Structural studies of EF-Tu complexes

    DEFF Research Database (Denmark)

    Johansen, Jesper Sanderhoff

    2011-01-01

    elongation factor, EF-Ts, catalyse the dissociation of GDP from EF-Tu which in turn allows EF-Tu to bind another GTP molecule. The resulting EF-Tu:GTP complex is then cable of forming a ternary complex with a new aa-tRNA which can then associate with the ribosome. During my time as a Ph. D. student I have...... and in manuscript I. G. anatolicus is a hyperthermophilic bacteria isolated from a thermal vent. The structure of G. anatolicus EF-Ts has been determined in complex with E. coli EF-Tu by X-ray crystallography to a resolution of 2.8 Å. The structure of G. anatolicus EF-Ts is more similar to the previously determined...... structures of E. coli and bovine mitochondrial EF-Ts rather than EF-Ts from the thermophilic bacteria T. thermophilus. The structure of G. anatolicus EF-Ts in complex with E. coli EF-Tu is presented in chapter 3 and in manuscript II. Mammalian mitochondria contain an independent genome which encodes 13...

  8. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  9. Vector Loop Algebra and Its Applications to Tu Hierarchy

    Institute of Scientific and Technical Information of China (English)

    WANG Yan

    2007-01-01

    A vector loop algebra and its extended loop algebra are proposed, which are devoted to obtaining the Tu hierarchy. By making use of the extended trace identity, the Hamiltonian structure of the Tu hierarchy is constructed.Furthermore, we apply the quadratic-form identity to the integrable coupling system of the Tu hierarchy.

  10. One-step purification of E. coli elongation factor Tu

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.; Degn, B

    1993-01-01

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one...

  11. Precursor for elongation factor Tu from Escherichia coli.

    OpenAIRE

    1986-01-01

    The tufA gene, one of two genes in Escherichia coli encoding elongation factor Tu (EF-Tu), was cloned into a ColE1-derived plasmid downstream of the lac promoter-operator. In cells carrying this plasmid, the synthesis of EF-Tu was increased four- to fivefold upon the addition of isopropyl-beta-D-thiogalactopyranoside (an inducer of the lac promoter). This condition led to the synthesis of a novel protein, called pTu, which comigrated with EF-Tu on a sodium dodecyl sulfate-polyacrylamide gel b...

  12. Artemisinin and Chinese medicine as Tu science.

    Science.gov (United States)

    Fu, Jia-Chen

    2017-07-07

    The story of discovery of artemisinin highlights the diversity of scientific values across time and space. Resituating artemisinin research within a broader temporal framework allows us to understand how Chinese drugs like qinghao came to articulate a space for scientific experimentation and innovation through its embodiment of alternating clusters of meanings associated with tu and yang within scientific discourse. Tu science, which was associated with terms like native, Chinese, local, rustic, mass, and crude, articulated a radical vision of science in the service of socialist revolutionary ideals. Yang science, which signified foreign, Western, elite, and professional, tended to bear the hallmarks of professionalism, transnational networks in education and training, and an emphasis on basic or foundational research. With respect to medical research, the case of artemisinin highlights how the constitution of socialist science as an interplay of tu and yang engendered different scientific values and parameters for scientific endeavor. Modern medical research in Maoist China could harness the productive energies of mass participation to technical expertise in its investigations of Chinese drugs, and under the banner of tu science, it became possible and scientifically legitimate to research Chinese drugs in ways that had previously provoked resistance and controversy. Copyright © 2017 Elsevier Ltd. All rights reserved.

  13. Efeitos de sessões educativas no uso das mochilas escolares em estudantes do ensino fundamental I Effects of educational sessions on school backpack use among elementary school students

    Directory of Open Access Journals (Sweden)

    SMS Fernandes

    2008-12-01

    Full Text Available OBJETIVO: Avaliar a modificação na quantidade de carga transportada, o modelo e o modo de transportar mochilas escolares após sessões educativas. MÉTODOS: Estudo de uma série de casos, com 99 crianças de sete a 11 anos do ensino fundamental, em escola particular da cidade São Paulo, São Paulo. Foram avaliadas a massa corporal (kg e estatura dos alunos (cm, quantidade de carga transportada nas mochilas (kg. Os modelos e os modos de transporte das mochilas foram avaliados por filmagem pré e pós-intervenção. Como medida de intervenção, os sujeitos (crianças, pais e professores foram submetidos a uma sessão educativa, que consistiu de orientações teóricas sobre coluna vertebral e transporte de carga. Para os escolares foi adicionada orientação prática das posturas corretas no transporte de carga. Os escolares receberam um reforço prático mensal por três meses. Pais e professores receberam reforço em folheto informativo e orientações na homepage da escola. Os dados foram analisados pelos testes estatísticos de igualdade de duas proporções e Wilcoxon. O nível de significância adotado foi 5%. RESULTADOS: O modelo de mochila modificou para duas alças de 46,5% para 60,6% (p=0,046, modo de transporte para ombro bilateral de 41,4% para 55,6% (p=0,047. A carga transportada nas mochilas diminuiu 2,66kg (p15% da massa corporal do aluno diminuiu (pOBJECTIVE: To evaluate the changes in loads carried, in the model of backpack used and in the way of carrying school backpacks after the implementation of an educational program. METHODS: This study was performed on 99 children aged seven to 11 years at elementary school level in a private school in São Paulo, São Paulo, Brazil. The subjects' body mass (kg and height (cm and the loads carried in their backpacks (kg were evaluated. The backpack models and the ways of carrying them were evaluated by filming before and after the intervention. The intervention program consisted of

  14. Structural studies of EF-Tu complexes

    DEFF Research Database (Denmark)

    Johansen, Jesper Sanderhoff

    2011-01-01

    proteins and 22 tRNAs and 2 rRNAs. The mammalian mitochondria have their own specialised translational system for maintaining the synthesis of the 13 proteins. The mammalian mitochondrial protein synthesis resembles the prokaryotic system more than the cytosolic system from eukaryotes. Some of the t...... and tRNASerGCU was produced by in vitro transcription. The ternary complex composed of EF-Tu:GDPNP:tRNASerGCU was reconstituted for crystallisation experiments. Unfortunately, we have not succeeding in crystallising neither the free components nor the ternary complex so far. The results of this project...

  15. Chloroplast EF-Tu and thermal aggregation of Rubisco activase

    Science.gov (United States)

    Chloroplast protein synthesis elongation factor, EF-Tu, has been implicated in heat tolerance in maize. The recombinant precursor of this protein, pre-EF-Tu, has been found to exhibit chaperone activity and protect heat-labile proteins, such as citrate synthase and malate dehydrogenase, from therma...

  16. Preparação do corpossom: atuação e voz concreta

    OpenAIRE

    Francisco Lauridsen Ribeiro

    2011-01-01

    Este trabalho lida com a explicitação da materialidade da voz do ator, através do emprego do termo \\'voz concreta\\'. Trata-se de uma investigação prática em que avaliamos recursos variados na preparação deste ator. Servimo-nos de diversas analogias e ferramentas: desde o emprego de termos oriundos de uma análise musical até a correspondência do desenho como expressão linear semelhante à voz. Foram também abordados como paradigma de concretude o canto improvisacional basco e a música concreta....

  17. Estudo e propostas de melhoria na preparação de kits na Bosch Termotecnologia

    OpenAIRE

    Trigo, Luís Filipe Rocha

    2009-01-01

    O presente trabalho resulta de um estágio curricular desenvolvido na Bosch Termotecnologia. O objectivo deste trabalho é o estudo da preparação de kits numa área específica da Bosch Termotecnologia, baseando-se no modelo de gestão Bosch Production System, os seus princípios e elementos. Foram particularmente considerados os princípios de melhoria contínua, a metodologia 5S, os dispositivos Poka-Yoke e as ferramentas básicas da Qualidade. O conjunto de acções de melhoria propostas vis...

  18. A influência da leitura do livro “Os Meninos das Costas Perfeitas”, na adoção de comportamentos saudáveis, relacionados com a mochila escolar

    OpenAIRE

    Silva, Sandra

    2013-01-01

    Introdução: O impacto do transporte diário das mochilas tornou-se uma área de preocupação da saúde pública, sendo necessária uma urgente mudança de comportamentos das crianças, educadores e cuidadores. Objetivos: O objetivo do presente estudo foi avaliar a influência da leitura do livro “Os Meninos das Costas Perfeitas” na mudança de comportamentos de estudantes do 1º e 4º anos. Métodos: Efetuou-se um estudo quase – experimental, onde se avaliou o efeito da leitura do livro,...

  19. Characterization of Elongation Factor Tu of Mycoplasma ovipneumoniae

    Directory of Open Access Journals (Sweden)

    Xuan Zhang, Yue-feng Chu, Ping Zhao, Peng-cheng Gao, Ying He, Nu Wang and Zhong-xin Lu*

    2013-11-01

    Full Text Available Mycoplasma ovipneumoniae is considered as an important pathogen of small ruminants, but its antigenic proteins are not well known so far. In this study, we cloned the EF-Tu gene of M. ovipneumoniae and analyzed the molecular features of the gene and its coding protein for the first time. The gene was then expressed in E.coli and the antigenicity of the coding protein was evaluated as well. The EF-Tu gene of M. ovipneumoniae is 1209 bp in length, encodes 402 amino acids, and shares the highest DNA sequence identity of 87.5% and deduced amino acid sequence identity of 97.8% with those of M. hyopneumoniae, respectively. The recombinant EF-Tu protein can react with the polyclonal antiserum of M. ovipneumoniae and can induce humoral immune responses in mice, which indicated that the EF-Tu may be used as a candidate protein in developing the technologies to control the disease.

  20. Métodos de Preparação e Propriedades de Vidros de Fluoretos

    OpenAIRE

    Noguchi, Yuri Yasuo

    2004-01-01

    Os vidros de fluoretos de metais pesados possuem usualmente muitas espécies catiônicas e muitas reações intermediárias ocorrem durante a sua fabricação. Por outro lado o resfriamento do fundido é muito rápido. Desta forma as propriedades do vidro dependem fortemente do caminho químico e da história térmica. Neste trabalho estudamos o efeito do caminho químico (método de preparação) sobre as propriedades térmicas e ópticas de vidros ZBLAN:Pr com a composição 57ZrF4-14BaF2-4LaF3-5AlF3-20NaF:xPr...

  1. TU PATERE LEGEM QUAM IPSE FECISTI

    CERN Multimedia

    Association du personnel

    2010-01-01

    In two judgments of February 2009, the ILO Administrative Tribunal (hereafter ILOAT) upheld CERN’s contract policy. In another case having given rise to a recent judgment (3 February 2010), the same problems, in essence, were raised concerning the illegal injustice which could result from the implementation of the policy in question. This time, the Tribunal decided in favour of our colleague, basing itself on the Latin maxim tu patere legem quam ipse fecisti, according to which any authority is bound by its own rules, as long as such rules have not been amended or abrogated. In fact, the Administration had informed the complainant that his application for a long-term contract would be examined for six slots, when in fact it was examined for only one. This judgment represents a victory for the complainant, for the Staff Association and its perseverance, and also for the principle according to which any organisation must keep its word and act in good faith. It shows that, without reversing its jud...

  2. EF-Tu dynamics during pre-translocation complex formation: EF-Tu·GDP exits the ribosome via two different pathways.

    Science.gov (United States)

    Liu, Wei; Chen, Chunlai; Kavaliauskas, Darius; Knudsen, Charlotte R; Goldman, Yale E; Cooperman, Barry S

    2015-10-30

    The G-protein EF-Tu, which undergoes a major conformational change when EF-Tu·GTP is converted to EF-Tu·GDP, forms part of an aminoacyl(aa)-tRNA·EF-Tu·GTP ternary complex (TC) that accelerates the binding of aa-tRNA to the ribosome during peptide elongation. Such binding, placing a portion of EF-Tu in contact with the GTPase Associated Center (GAC), is followed by GTP hydrolysis and Pi release, and results in formation of a pretranslocation (PRE) complex. Although tRNA movement through the ribosome during PRE complex formation has been extensively studied, comparatively little is known about the dynamics of EF-Tu interaction with either the ribosome or aa-tRNA. Here we examine these dynamics, utilizing ensemble and single molecule assays employing fluorescent labeled derivatives of EF-Tu, tRNA, and the ribosome to measure changes in either FRET efficiency or fluorescence intensity during PRE complex formation. Our results indicate that ribosome-bound EF-Tu separates from the GAC prior to its full separation from aa-tRNA, and suggest that EF-Tu·GDP dissociates from the ribosome by two different pathways. These pathways correspond to either reversible EF-Tu·GDP dissociation from the ribosome prior to the major conformational change in EF-Tu that follows GTP hydrolysis, or irreversible dissociation after or concomitant with this conformational change.

  3. Caracterização de preparações extrativas obtidas de Passiflora alata Curtis

    Directory of Open Access Journals (Sweden)

    ANDREAS S. L. MENDEZ

    2011-06-01

    Full Text Available O presente trabalho teve como objetivo fazer uma análise comparativa da qualidade de preparações extrativas obtidas de Passiflora alata Curtis, além de descrever esse processo. O extrato líquido foi obtido por refluxo utilizando água como solvente em proporção 1:10. A preparação líquida foi submetida à secagem em spray dryer utilizando aerosil® e fosfato de cálcio como adjuvantes tecnológicos. A eficiência de extração foi avaliada através da determinação do resíduo seco e do teor de flavonoides totais por técnica de espectrofotometria no UV. O extrato seco foi avaliado quanto à viabilidade tecnológica a partir da determinação das características de granulometria e da estabilidade em ambientes de umidade relativa controlada. O teor de flavonoides totais para o extrato seco apresentou redução quando comparado à solução extrativa, indicando possível influência do processo de secagem na composição do material. Os resultados de caracterização tecnológica demonstraram que o processo de secagem utilizado garante uma uniformidade de tamanho particular, embora seja observada uma tendência de higroscopicidade do material em ambientes de elevada umidade relativa. As técnicas empregadas se mostraram viáveis para uso em ensaios quali-quantitativos aplicados à determinação da qualidade dos extratos desenvolvidos. Palavras-chave: Passiflora alata Curtis; Preparações extrativas; Spray-drying; Flavonoides totais; Caracterização tecnológica. ABSTRACT Characterization of extracts prepared from Passiflora alata Curtis This paper describes a process to prepare liquid and dried aqueous extracts from aerial parts of Passiflora alata Curtis (sweet passionfruit and reports a comparative analysis of the extractive preparations obtained. The extractive solution was obtained by refluxing the plant material with water in the proportion 1:10 (w/v. The aqueous extract was dried in a spray-dryer, with Aerosil® and calcium

  4. College via iTunes U: TU Delft deelt graag kennis

    NARCIS (Netherlands)

    Suijker, P.

    2010-01-01

    De TU Delft publiceert lezingen, colleges en informatieve films op iTunes U van Apple. De auteur beschrijft waarom de TU Delft gekozen heeft voor iTunes U en op welke manier het project tot stand is gekomen.

  5. Elongation Factor-Tu (EF-Tu) proteins structural stability and bioinformatics in ancestral gene reconstruction

    Science.gov (United States)

    Dehipawala, Sunil; Nguyen, A.; Tremberger, G.; Cheung, E.; Schneider, P.; Lieberman, D.; Holden, T.; Cheung, T.

    2013-09-01

    A paleo-experimental evolution report on elongation factor EF-Tu structural stability results has provided an opportunity to rewind the tape of life using the ancestral protein sequence reconstruction modeling approach; consistent with the book of life dogma in current biology and being an important component in the astrobiology community. Fractal dimension via the Higuchi fractal method and Shannon entropy of the DNA sequence classification could be used in a diagram that serves as a simple summary. Results from biomedical gene research provide examples on the diagram methodology. Comparisons between biomedical genes such as EEF2 (elongation factor 2 human, mouse, etc), WDR85 in epigenetics, HAR1 in human specificity, DLG1 in cognitive skill, and HLA-C in mosquito bite immunology with EF Tu DNA sequences have accounted for the reported circular dichroism thermo-stability data systematically; the results also infer a relatively less volatility geologic time period from 2 to 3 Gyr from adaptation viewpoint. Comparison to Thermotoga maritima MSB8 and Psychrobacter shows that Thermus thermophilus HB8 EF-Tu calibration sequence could be an outlier, consistent with free energy calculation by NUPACK. Diagram methodology allows computer simulation studies and HAR1 shows about 0.5% probability from chimp to human in terms of diagram location, and SNP simulation results such as amoebic meningoencephalitis NAF1 suggest correlation. Extensions to the studies of the translation and transcription elongation factor sequences in Megavirus Chiliensis, Megavirus Lba and Pandoravirus show that the studied Pandoravirus sequence could be an outlier with the highest fractal dimension and lowest entropy, as compared to chicken as a deviant in the DNMT3A DNA methylation gene sequences from zebrafish to human and to the less than one percent probability in computer simulation using the HAR1 0.5% probability as reference. The diagram methodology would be useful in ancestral gene

  6. Trust Management in P2P Systems Using Standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential sto

  7. Trust Management in P2P Systems Using Standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential

  8. Trust Management in P2P systems using Standard TuLiP

    NARCIS (Netherlands)

    Czenko, M.R.; Doumen, J.M.; Etalle, Sandro

    2008-01-01

    In this paper we introduce Standard TuLiP - a new logic based Trust Management system. In Standard TuLiP, security decisions are based on security credentials, which can be issued by different entities and stored at different locations. Standard TuLiP directly supports the distributed credential

  9. Ribosome-induced changes in elongation factor Tu conformation control GTP hydrolysis

    DEFF Research Database (Denmark)

    Villa, Elizabeth; Sengupta, Jayati; Trabuco, Leonard G.

    2009-01-01

    In translation, elongation factor Tu (EF-Tu) molecules deliver aminoacyl-tRNAs to the mRNA-programmed ribosome. The GTPase activity of EF-Tu is triggered by ribosome-induced conformational changes of the factor that play a pivotal role in the selection of the cognate aminoacyl-tRNAs. We present a...

  10. Functional studies of elongation factor Tu from Escherichia coli : Site-directed mutagenesis and antibiotic action

    NARCIS (Netherlands)

    Krab, Ivo Maarten

    2001-01-01

    This PhD thesis describes several studies into the structure and function of Escherichia coli Elongation Factor Tu (EF-Tu). EF-Tu plays a central role in the bacterial protein synthesis machinery as the carrier of "coded building blocks" for protein synthesis, aminoacylated tRNA (aa-tRNA). Without E

  11. The Impending Demise of "tu" in Montevideo, Uruguay

    Science.gov (United States)

    Weyers, Joseph R.

    2009-01-01

    Since the early 1960s, the "tuteo" (T-T), and particularly the use of tonic "tu" with the "vos" verbal morphology (T-V) has been accepted as typical of the speech of Montevideo, Uruguay, a region generally classified as "voseante". This paper reports on the results of 117 rapid anonymous interviews conducted in Montevideo to document the status of…

  12. The Impending Demise of "tu" in Montevideo, Uruguay

    Science.gov (United States)

    Weyers, Joseph R.

    2009-01-01

    Since the early 1960s, the "tuteo" (T-T), and particularly the use of tonic "tu" with the "vos" verbal morphology (T-V) has been accepted as typical of the speech of Montevideo, Uruguay, a region generally classified as "voseante". This paper reports on the results of 117 rapid anonymous interviews conducted…

  13. (Average-) convexity of common pool and oligopoly TU-games

    NARCIS (Netherlands)

    Driessen, T.S.H.; Meinhardt, H.

    2000-01-01

    The paper studies both the convexity and average-convexity properties for a particular class of cooperative TU-games called common pool games. The common pool situation involves a cost function as well as a (weakly decreasing) average joint production function. Firstly, it is shown that, if the rele

  14. Problem-Centered Design and Personal Teaching Style: An Exploratory Study of Youguang Tu's Course on Philosophy of Education

    Science.gov (United States)

    Lei, Hongde

    2016-01-01

    Youguang Tu is a contemporary Chinese philosopher of education. His course on philosophy of education had a significant impact on his students. This exploratory study examines how Tu designed and taught this course. Ultimately, there are two reasons why Tu's course had such a significant influence on his students. The first is that Tu used…

  15. A graduação em Psicologia prepara para o trabalho no hospital?

    Directory of Open Access Journals (Sweden)

    Zeila Facci Torezan

    Full Text Available Considerando a Psicologia hospitalar uma especialidade em expansão no campo da Psicologia da saúde, o presente trabalho resulta de uma pesquisa exploratória sobre a preparação na graduação dos psicólogos hospitalares, que verificou a relação da formação acadêmica com a prática em hospitais gerais na região de Londrina - PR. Participaram desta pesquisa 10 psicólogos hospitalares com vínculo empregatício de no mínimo seis meses e três coordenadores de cursos de graduação em Psicologia de Londrina. Para a coleta dos dados, foram utilizados dois questionários de entrevista semiestruturada que continham questões abertas e fechadas. O primeiro questionário levantou dados sobre a formação acadêmica e as funções desenvolvidas pelos psicólogos nos hospitais, e o segundo buscou informações referentes às oportunidades de formação voltadas para a área de saúde e ofertadas pelos cursos de Psicologia pesquisados. Também foi realizada consulta aos projetos pedagógicos dos referidos cursos. No universo pesquisado, constatou-se que, em geral, a formação na graduação em Psicologia não contempla as particularidades necessárias para o trabalho em hospital geral.

  16. Preparações radiofarmacêuticas e suas aplicações Radiopharmaceuticals and applications

    Directory of Open Access Journals (Sweden)

    Rita Oliveira

    2006-06-01

    Full Text Available Os radiofármacos são compostos, sem ação farmacológica, que têm na sua composição um radionuclídeo e são utilizados em Medicina Nuclear para diagnóstico e terapia de várias doenças. Para aplicações de diagnóstico em Medicina Nuclear utilizam-se radiofármacos que apresentam na sua constituição radionuclídeos emissores de radiação gama ou emissores de pósitrons (beta+, já que o decaimento destes radionuclídeos dá origem a radiação eletromagnética penetrante, que consegue atravessar os tecidos e pode ser detectada externamente. Os radiofármacos para terapia devem incluir na sua composição um radionuclídeo emissor de partículas ionizantes (a , b- ou elétrons Auger, pois a sua ação se baseia na destruição seletiva de tecidos. Existem dois métodos tomográficos para aquisição de imagens em Medicina Nuclear: o SPECT (Tomografia Computarizada de Emissão de Fóton Único, que utiliza radionuclídeos emissores gama (99mTc, 123I, 67Ga, 201Tl e o PET (Tomografia por Emissão de Pósitrons, que usa radionuclídeos emissores de pósitrons ( 11C, 13N, 15O, 18F. Os radiofármacos podem ser classificados em radiofármacos de perfusão (ou 1ª geração e radiofármacos específicos (ou 2ª geração. Os radiofármacos de perfusão são transportados no sangue e atingem o órgão alvo na proporção do fluxo sanguíneo. Os radiofármacos ditos específicos contêm molécula biologicamente ativa, que se liga a receptores celulares e que deve manter a sua bioespecificidade mesmo após ligação ao radionuclídeo. Assim, nestes radiofármacos, a fixação em tecidos ou órgãos é determinada pela capacidade da biomolécula de reconhecer receptores presentes nessas estruturas biológicas. As preparações radiofarmacêuticas são obtidas prontas para uso, em kits frios ou em preparações autólogas. De acordo com o tipo de preparação, existe um processo de controle de qualidade próprio. A maior parte dos radiof

  17. Roles of Protein Synthesis Elongation Factor EF-Tu in Heat Tolerance in Plants

    Directory of Open Access Journals (Sweden)

    Jianming Fu

    2012-01-01

    Full Text Available EF-Tu proteins of plastids, mitochondria, and the cytosolic counterpart EF-1α in plants, as well as EF-Tu proteins of bacteria, are highly conserved and multifunctional. The functions of EF-Tu include transporting the aminoacyl-tRNA complex to the A site of the ribosome during protein biosynthesis; chaperone activity in protecting other proteins from aggregation caused by environmental stresses, facilitating renaturation of proteins when conditions return to normal; displaying a protein disulfide isomerase activity; participating in the degradation of N-terminally blocked proteins by the proteasome; eliciting innate immunity and triggering resistance to pathogenic bacteria in plants; participating in transcription when an E. coli host is infected with phages. EF-Tu genes are upregulated by abiotic stresses in plants, and EF-Tu plays important role in stress responses. Expression of a plant EF-Tu gene confers heat tolerance in E. coli, maize knock-out EF-Tu null mutants are heat susceptible, and over-expression of an EF-Tu gene improves heat tolerance in crop plants. This review paper summarizes the current knowledge of EF-Tu proteins in stress responses in plants and progress on application of EF-Tu for developing crop varieties tolerant to abiotic stresses, such as high temperatures.

  18. Rotas de preparação de intermediarios para a sintese de alcaloides indolicos e extração de produtos naturais relacionados

    OpenAIRE

    Antonio Carlos Siani

    1980-01-01

    Resumo: No presente trabalho são apresentadas rotas de preparação de moléculas intermediárias chaves (sintones) que posteriormente possam conduzir à alcalóides indólicos do tipo Vinca (vincamina, erbunamonina - hipotensores cerebrais) e Stricnos (estricnina). O projeto de pesquisa resume-se em duas etapas: a preparação de substratos imídicos (N-(o) toluilglutarimida, N-(o)toluilglutaconimida, N-(o) toluilmaleimida e derivados) e no comportamento desses substratos em meio básico. A ação da bas...

  19. A preparação do Ministério Público para a Justiça Constitucional no Brasil

    OpenAIRE

    Melo, Marconi Antas Falcone de

    2012-01-01

    O presente trabalho objetiva investigar a preparação do Ministério Público para a atuação racional no sistema de justiça constitucional no Brasil. Não se trata de mais um manual sobre controle de constitucionalidade. O foco na preparação do Ministério Público para o eficiente exercício é o diferencial do trabalho. A escolha do tema justifica-se em virtude do amadorismo que vige no Ministério Público brasileiro em tal atribuição. Dentre as principais hipóteses articuladas, basei...

  20. Abre tu nevera! Aplicación para Android

    OpenAIRE

    Cañizares Fernandez, Rosa

    2014-01-01

    Aquí presento mi PFC Abre tu nevera! Consiste en un recetario paso a paso basado en los productos que tenemos en la despensa. Además cuenta de una gestión de listas de la compra. En el código fuente podremos encontrar una carpeta con el código fuente del proyecto Android y la app lista para instalar.

  1. Reconstitution of Qbeta RNA replicase from a covalently bonded elongation factor Tu-Ts complex

    DEFF Research Database (Denmark)

    Brown, S; Blumenthal, T

    1976-01-01

    (C) and Qbeta RNA normally and had approximately the same specific activity as control enzyme. Denatured Qbeta replicase formed with crosslinked EF-Tu-Ts was found to renature much more rapidly than untreated enzyme and, in contrast to normal replicase, its renaturation was not inhibited by GDP. The results...... by its ability to replace EF-Tu and EF-Ts in the renaturation of denatured Qbeta replicase. A sample of Qbeta replicase with crosslinked EF-Tu-Ts replacing the individual elongation factors was prepared. Although it lacked EF-Tu and EF-Ts activities, it could initiate transcription of both poly...

  2. Association of Acinetobacter baumannii EF-Tu with Cell Surface, Outer Membrane Vesicles, and Fibronectin

    Directory of Open Access Journals (Sweden)

    Shatha F. Dallo

    2012-01-01

    Full Text Available A conundrum has long lingered over association of cytosol elongation factor Tu (EF-Tu with bacterial surface. Here we investigated it with Acinetobacter baumannii, an emerging opportunistic pathogen associated with a wide spectrum of infectious diseases. The gene for A. baumannii EF-Tu was sequenced, and recombinant EF-Tu was purified for antibody development. EF-Tu on the bacterial surface and the outer membrane vesicles (OMVs was revealed by immune electron microscopy, and its presence in the outer membrane (OM and the OMV subproteomes was verified by Western blotting with the EF-Tu antibodies and confirmed by proteomic analyses. EF-Tu in the OM and the OMV subproteomes bound to fibronectin as detected by Western blot and confirmed by a label-free real-time optical sensor. The sensor that originates from photonic crystal structure in a total-Internal-reflection (PC-TIR configuration was functionalized with fibronectin for characterizing EF-Tu binding. Altogether, with a novel combination of immunological, proteomical, and biophysical assays, these results suggest association of A. baumannii EF-Tu with the bacterial cell surface, OMVs, and fibronectin.

  3. Sensory analysis of hydrolysed meat preparations Análise sensorial de preparações com hidrolisados de carne

    Directory of Open Access Journals (Sweden)

    Maria Elisabeth Machado Pinto E Silva

    2010-06-01

    Full Text Available The use of hydrolysed meat in diets contributes to the improvement of protein, vitamin and mineral supply. This work aims at checking the acceptance pattern in meat hydrolysates. Four preparations have been developed with three types of hydrolysates in domestic-like conditions. Acceptance was verified by means of sensory analysis using the nine-point hedonic scale. Sensory tests have been carried out in three sessions (according to the kind of hydrolysates. In the evaluation file, information on age groups has been included. The statistical analysis has been made by ANOVA and Tukey test. The best accepted preparation have been the turkey and chicken hydrolysed balls. Hydrolysates can be used in many different kinds of preparations, but it is necessary to know both the age group it will be used to and its sensory and chemical-physical features to ensure the taste and the original appearance of the final product.A utilização de hidrolisados de carne em dietas melhora seu conteúdo protéico, de vitaminas e minerais. O objetivo do presente trabalho foi avaliar a aceitação de hidrolisados de carne. Quatro preparações foram desenvolvidas com três tipos de hidrolisados em condições similares às domésticas. . A aceitação foi avaliada com uso de escala hedônica de 9 pontos. Os testes foram realizados em três sessões (de acordo com o tipo de hidrolisado e, incluiu-se na ficha de avaliação informações de idade. A análise estatística foi realizada por ANOVA e teste de Tukey. As preparações mais aceitas foram os bolinhos com hidrolisados de peru e frango. Os hidrolisados podem ser utilizados em diversas preparações, sendo necessário o conhecimento da faixa etária a qual se destinam, suas características sensoriais e físico-químicas, para garantir o sabor e a aparência do produto final.

  4. Preparação da assistência farmacêutica para desastres: um estudo em cinco municípios brasileiros

    Directory of Open Access Journals (Sweden)

    Paula Pimenta-de-Souza

    2014-09-01

    Full Text Available Uma série de municípios brasileiros foram acometidos por desastres nos últimos anos. Neste sentido, os gestores precisam investir na preparação para mitigar o impacto dos eventos e aliviar o dano causado. A preparação para desastres deve ser realizada em diversos frentes, inclusive no setor saúde, em que se insere a assistência farmacêutica. Objetivou-se descrever e analisar a preparação da assistência farmacêutica para desastres em municípios recentemente acometidos por desastres. Para tal, foi realizado um estudo com desenho transversal em cinco municípios brasileiros, em duas regiões do país. Foram analisadas fontes documentais, matérias jornalísticas e bases de dado de acesso público, além de entrevistas a atores-chave da AF através de um instrumento baseado em um modelo lógico e indicadores. Foram identificadas poucas medidas de preparação da assistência farmacêutica nos municípios estudados. Ainda que o histórico dos municípios esteja ligado a eventos que redundaram em desastres, a gestão municipal da assistência farmacêutica não foi capaz de preparar-se. Espera-se que a apresentação da experiência desses municípios favoreça a preparação da assistência farmacêutica em outros municípios do país.

  5. Sobrevivencia de las fases preparasíticas de nematodos gastrointestinales en bocashi elaborado con estiércol vacuno.

    Directory of Open Access Journals (Sweden)

    Martha Orozco-Aceves

    2015-12-01

    Full Text Available El objetivo del presente trabajo fue determinar la sobrevivencia de las fases preparasíticas (huevos y larvas de nematodos gastrointestinales (NG en bocashi elaborado con estiércol vacuno. La investigación se realizó en la Universidad Nacional de Costa Rica en el año 2010. Abono orgánico tipo bocashi fue elaborado utilizando estiércol vacuno procedente de una finca lechera localizada en Poasito de Alajuela, Costa Rica. El estiércol contenía 600 huevos por gramo de heces (hpg de los NG de la suborden Strongylida, < 50 hpg de Strongyloides papillosus y < 50 hpg de Trichuris spp. Cuando el bocashi estuvo elaborado, veinte muestras fueron tomadas aleatoriamente y procesadas mediante el método cualitativo de Sheater y el cuantitativo de McMaster para determinar la presencia de huevos de NG y contabilizarlos. Adicionalmente, se montaron coprocultivos y se extrajeron los nematodos totales para determinar la presencia de larvas de NG. En el bocashi las cantidades de huevos de NG fueron bajas y no fue posible la cuantificación por el método estándar de McMaster. En todos los casos los huevos mostraron signos de degradación. Ninguna larva de NG fue observada en los coprocultivos ni en la extracción de nematodos totales. El bocashi contenía un alto número de nematodos bacteriófagos, cuya presencia es positiva. Las condiciones de elaboración del bocashi lograron disminuir las poblaciones de fases preparasíticas de NG y favorecieron la proliferación de organismos benéficos.

  6. Preparação para a aposentadoria como parte da educação ao longo da vida

    Directory of Open Access Journals (Sweden)

    Lucia Helena de Freitas Pinho França

    Full Text Available O processo de globalização trouxe para as grandes organizações uma série de consequências, entre as quais a prática das aposentadorias incentivadas, em que diversos trabalhadores experientes foram dispensados, muitos sem qualquer preparação para uma vida que pode durar mais do que o tempo dedicado ao trabalho. Hoje muitas organizações já reconhecem o valor dos trabalhadores mais velhos, que, se atualizados, podem continuar tão motivados quanto já demonstraram em capacidade e experiência. Entretanto, alguns precisam ou desejam se aposentar, aproveitando o tempo livre para realizar atividades de desenvolvimento pessoal, de lazer, de cuidado com a saúde, desenvolvimento de projetos pessoais, relacionamentos sociais e familiares e mesmo para o engajamento em uma atividade profissional mais prazerosa. Este artigo pretende oferecer uma perspectiva do envelhecimento dos trabalhadores nas organizações e do desafio de lidar com a aposentadoria, analisando-se aspectos e variáveis que podem facilitar ou dificultar o bem-estar das pessoas nessa transição. Mais do que focar nas teorias que estariam fundamentando os Programas de Preparação para a Aposentadoria (PPAs, o artigo apresenta algumas pesquisas empíricas, de forma a incentivar a reflexão dos psicólogos sociais, organizacionais e orientadores profissionais sobre as suas possibilidades de inserção nessa área de atuação.

  7. The gammaTuRC Nanomachine Mechanism and Future Applications

    Science.gov (United States)

    Riehlman, Timothy D.

    The complexity and precision of the eukaryotic cell's cytoskeletal network is unrivaled by any man-made systems, perfected by billions of years of evolution, mastering elegant processes of self-assembly, error correction, and self-repair. Understanding the capabilities of these networks will have important and far reaching applications in human medicine by aiding our understanding of developmental processes, cellular division, and disease mechanisms, and through biomimicry will provide insights for biosynthetic manufacturing at the nanoscale and across scales. My research utilizes cross species techniques from Human to the model organism of Fission Yeast to investigate the structure and mechanisms of the g-tubulin ring complex (gTuRC). The gTuRC is a highly conserved eukaryotic multiprotein complex serving as a microtubule organizing center (MTOC) responsible for microtubule nucleation through templating, regulation of dynamics, and establishment of microtubule polarity. Microtubules are 25 nm diameter dynamic flexible polymers of a/b-tubulin heterodimers that function as scaffolds, force generators, distributors, and intracellular highways. The microtubule cytoskeleton is essential for numerous fundamental cellular processes such as mitotic division of chromosomes and cell division, organelle distribution within the cell, cell signaling, and cell shape. This incredible diversity in functions is made possible in part due to molecular motor Kinesin-like proteins (Klps), which allow expansion into more specialized neural, immune, and ciliated cell functions. Combined, the MTOC, microtubules, and Klps represent ideal microtubule cytoskeleton protein (MCP) modular components for in vitro biomimicry towards generation of adaptable patterned networks for human designed applications. My research investigates the hypothesis that a mechanistic understanding of conserved MTOC gTuRC mechanisms will help us understand dynamic cellular nanomachines and their ability to self

  8. The Measure your World / Mide tu Mundo Project

    Science.gov (United States)

    Hojman, S.; Johnson, R. M.; Meymaris, K. K.; Ward, D. L.; Russell, R.; Genyuk, J.; Lagrave, M.; Henderson, S.; Ostrosky, J.; Martinez, M.

    2007-12-01

    Over 22 centuries ago Erathostenes devised a method to determine the Earth radius. Measure your World / Mide tu Mundo is a joint venture lead by Windows to the Universe/Ventanas al Universo in the United States of America, EducaRed in Chile and RedEscolar in Mexico seeking to partner teams of students, teachers and parents from the three countries in a collaborative effort to determine the Earth circumference by sharing the results of their measurements, and exchanging cultural information. Data are collected from September 29 through October 7, 2007. A report of the whole experience will be presented.

  9. The Danish National Travel Survey - declaration of variables : TU 2006-13, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-13 version 2 covering data from the period May 2006 until 30. April 2014. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  10. The Danish National Travel Survey - declaration of variables : TU 2006-10, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Haunstrup, Brian

    This record documents the reported data in the data set TU 2006-10 version 1 covering data from the period May 2006 until 30 April 2011. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  11. The Danish National Travel Survey - declaration of variables TU 2006-12, version 2 : Documentation note

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-12 version 1 covering data from the period May 2006 until 3o April 2013. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  12. The Danish National Travel Survey - declaration of variables : TU 2006-12, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar; Skougaard, Britt Zoëga

    This record documents the reported data in the data set TU 2006-12 version 1 covering data from the period May 2006 until 31. December 2012. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  13. The Danish National Travel Survey - declaration of variables TU 2006-11, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    This record documents the reported data in the data set TU 2006-11 version 2 covering data from the period May 2006 until 30. April 2012. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  14. The Danish National Travel Survey - declaration of variables TU 2006-11, version 1

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    This record documents the reported data in the data set TU 2006-11 version 1 covering data from the period May 2006 until 31 December 2011. Please refer to our website for the most up-to-date documentation of the latest TU data. This record follows, where possible, the data set and is not updated...

  15. Intestinal, portal, and peripheral profiles of daikenchuto (TU-100)'s active ingredients after oral administration.

    Science.gov (United States)

    Watanabe, Junko; Kaifuchi, Noriko; Kushida, Hirotaka; Matsumoto, Takashi; Fukutake, Miwako; Nishiyama, Mitsue; Yamamoto, Masahiro; Kono, Toru

    2015-10-01

    A pharmaceutical grade Japanese traditional medicine, daikenchuto (TU-100), consisting of Japanese pepper, processed ginger, and ginseng, has been widely used for various intestinal disorders in Japan and now under development as a new therapeutic drug in the US. It is suggested that TU-100 ingredients exert pharmacological effects on intestines via two routes, from the luminal side before absorption and the peripheral blood stream after absorption. Therefore, in order to fully understand the pharmacological actions of TU-100, it is critically important to know the intraluminal amounts and forms of ingested TU-100 ingredients. In the present study, after administrating TU-100 to rats, the concentrations of TU-100 ingredients and their conjugates in the peripheral and portal blood and ileal contents were determined by LC-MS/MS. Next, TU-100 was administered to patients with ileostomy bags, but whose small intestines are diagnosed as healthy, and the ingredients/conjugates in the ileal effluent were analyzed. The results suggest that: (1) Pepper ingredients hydroxysanshools are rapidly absorbed and enter systemic circulation, (2) Ginseng ingredients ginsenosides are transported to the colon with the least absorption, (3) Ginger ingredients gingerols are absorbed and some conjugated in the small intestine and transported via the portal vein. While only a small amount of gingerols/gingerol conjugates enter systemic circulation, considerable amounts reappear in the small intestine. Thus, the effect of TU-100 on the intestines is believed to be a composite of multiple actions by multiple compounds supplied via multiple routes.

  16. Data of evolutionary structure change: 1TU8B-2AAWC [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available n>1TU8B SIKSQ---FQFGQ > GGG--- re... C 2AAWC KNFKKEKDTPFEQ ...>HHHHHH > ATOM 2082 CA LYS C 46 3.335 27.932 37.753 1.00 ...bChain> 1TU8B LLATR----GNGRN >HGGG ----GGG ...tryIDChain> LLKKNHTNNNNDKY >HHHH

  17. Test facility development for testing of micro-thrusters at TU-Delft

    NARCIS (Netherlands)

    Zandbergen, B.T.C.; Janssens, S.; Valente, F.; Perez-Grande, D.; Koopmans, R.

    2010-01-01

    The development of highly integrated and compact micro-satellites and the need for a higher precision of positioning and attitude control in space missions has stimulated the development of micro-propulsion systems. At TU-Delft, investigations are focusing on non-chemical, thermal propulsion. TU-Del

  18. The role of Glu259 in Escherichia coli elongation factor Tu in ternary complex formation

    DEFF Research Database (Denmark)

    Nautrup Pedersen, Gitte; Rattenborg, Thomas; Knudsen, Charlotte Rohde;

    1998-01-01

    Determination of the crystal structure of the ternary complex formed between elongation factor Tu:GTP and aminoacylated tRNA revealed three regions of interaction between elongation factor Tu and tRNA. The structure indicates that the conserved glutamic acid at position 271 in Thermus aquaticus EF......-Tu could be involved in the binding of the 3' CCA-Phe end of the aminoacylated tRNA. Therefore, the corresponding residue, Glu259, of Escherichia coli EF-Tu was mutated into alanine, aspartic acid, glutamine and tyrosine, in order to substantiate the crystallographic structural evidence and to obtain...... of interaction with tRNA, while mutation to tyrosine abolished completely the interaction with tRNA. Finally, mutation to glutamine resulted in an elongation factor Tu variant behaving like the wild type. In conclusion, the environment around the site binding the CCA-Phe end of the tRNA is very restricted...

  19. Análisis a una Figura Tradicional de las Mochilas Arhuacas: comunidad Indígena Arhuaca. Sierra Nevada de Santa Marta, Colombia. Analysis to one Traditional Figure of the Arhuacas Knapsacks: indigenous community Arhuaca. Sierra Nevada of Santa Marta, Colombia.

    Directory of Open Access Journals (Sweden)

    Armando Aroca Araújo

    2008-10-01

    Full Text Available En este artículo se mostrará el análisis de Háku, la serpiente de cascabel, una de las dieciséis Figuras Tradicionales que tejen las mujeres arhuacas en sus mochilas. Este análisis, es un producto de tres años de investigación para obtener el título de maestría en Educación Matemática, y de paso presenta implícitamente una propuesta etnomatemática de cómo abordar un objeto de estudio anclado en un contexto cultural diferente. Este análisis, que es multidisciplinar, presentará algunos aspectos que pretenden contribuir a la comprensión del significado cultural y social que tiene el objeto de estudio en referencia. Palabras claves: Etnomatemáticas. Análisis. Configuración geométrica. Mochilas arhuacas. Háku.This article will show the analysis of Háku, the rattlesnake, one of the sixteen Traditional Figures that the arhuacas women knit on their knapsacks. This analysis is a result of three years of investigation to obtain the title of masters in Mathematical Education, and it implicitly presents an ethnomathematic proposal of how to approach an object of study in a different cultural context. This analysis, which is multidiscipline, will present some aspects that try to contribute to the understanding of the cultural and social meaning that the object of study in reference has. Keywords: Ethnomathematics. Analysis. Geometric configuration. Arhuacas knapsacks. Háku.

  20. Physical Characterization of the Binary Asteroid 66146 (1998 Tu3)

    Science.gov (United States)

    Truong, Thien-Tin; Hicks, M.; Mayes, D.; Barajas, T.; Garcia, K.

    2011-01-01

    The near-Earth asteroid 66146 (1998 TU3) was discovered on 1998 October 13 by the LINEAR NEO survey (MPEC 1998-U03). We obtained five nights of Bessel BVRI observations (2010 Aug 6,7,10,12,13 UT) and one night of Bessel R (August 8 PST) at the JPL Table Mountain Observatory (TMO) 0.6-m telescope near Wrightwood, California. These observations were obtained as part of our ongoing survey at TMO of Potentially Hazardous Asteroids (PHAs), planetary radar targets, and low delta-V near-Earth asteroids (NEOs). The object's rotationally averaged colors (B-R=1.238+/-0.011 mag; V-R=0.440+/-0.008 mag; R-I=0.275+/-0.010 mag) were found most compatible with an Sk-type spectral classification (Bus Taxonomy)/S-type (Tholen Taxonomy). This association was obtained through a comparison of our colors with the 1341 asteroid spectra in the SMASS II database (Bus & Binzel 2002). Our classification differs significantly from the Q-type taxonomy reported by Whitely (2002). Assuming a solar phase parameter g=0.15 we performed a period search using standard Fourier techniques. We found a best-fit rotational period Psyn=2.378+/-0.001 hr, in excellent agreement with the 2.3779+/-0.0004 period determined by Richards et al. (2007). The dispersion in the phased single period lightcurve strongly suggests that 1998 TU3 is be a binary system, with variations in observed flux caused by an unresolved, tidally locked secondary companion. Fitting a 2-period model as described by Pravec et al. (2000), we found that our photometry agrees well with a binary model (P1=2.378+/-0.01 hr, P2=28.28+/-0.05 hr). We have three additional nights scheduled for this object at TMO (Oct 8, 9, 10 2010 UT), extending our solar phase coverage and allowing us to refine our rotational models. 1998 TU3 will experience an exceptional apparition in 2012. This object may be a good candidate for shape/pole modeling via lightcurve inversion, especially if photometry can be obtained from both northern and southern hemispheres. We

  1. Modelos lineares e não lineares inteiros para problemas da mochila bidimensional restrita a 2 estágios Linear and nonlinear integer models for constrained two-stage two-dimensional knapsack problems

    Directory of Open Access Journals (Sweden)

    Horacio Hideki Yanasse

    2013-01-01

    Full Text Available Neste trabalho revemos alguns modelos lineares e não lineares inteiros para gerar padrões de corte bidimensionais guilhotinados de 2 estágios, incluindo os casos exato e não exato e restrito e irrestrito. Esses problemas são casos particulares do problema da mochila bidimensional. Apresentamos também novos modelos para gerar esses padrões de corte, baseados em adaptações ou extensões de modelos para gerar padrões de corte bidimensionais restritos 1-grupo. Padrões 2 estágios aparecem em diferentes processos de corte, como, por exemplo, em indústrias de móveis e de chapas de madeira. Os modelos são úteis para a pesquisa e o desenvolvimento de métodos de solução mais eficientes, explorando estruturas particulares, a decomposição do modelo, relaxações do modelo etc. Eles também são úteis para a avaliação do desempenho de heurísticas, já que permitem (pelo menos para problemas de tamanho moderado uma estimativa do gap de otimalidade de soluções obtidas por heurísticas. Para ilustrar a aplicação dos modelos, analisamos os resultados de alguns experimentos computacionais com exemplos da literatura e outros gerados aleatoriamente. Os resultados foram produzidos usando um software comercial conhecido e mostram que o esforço computacional necessário para resolver os modelos pode ser bastante diferente.In this work we review some linear and nonlinear integer models to generate two stage two-dimensional guillotine cutting patterns, including the constrained, non constrained, exact and non exact cases. These problems are particular cases of the two dimensional knapsack problems. We also present new models to generate these cutting patterns, based on adaptations and extensions of models that generate one-group constrained two dimensional cutting patterns. Two stage patterns arise in different cutting processes like, for instance, in the furniture industry and wooden hardboards. The models are useful for the research and

  2. Warriors for Civilisation: Naguib Mahfouz, Andrei Tarkovsky, Tu Weiming and their Western Counterparts

    OpenAIRE

    Keir, Jonathan

    2014-01-01

    This thesis covers the decline and fall of the civilisation narrative in 20th Century Western humanities before exploring the work of three non-Western figures - Egyptian novelist Naguib Mahfouz, Russian film director Andrei Tarkovsky and Chinese philosopher Tu Weiming - who defend the concept of civilisation in their work. The views of Mahfouz, Tarkovsky and Tu can be viewed as consistent with each other and with those of contemporary Western intellectuals including John Armstrong, Martha Nu...

  3. A mechanism-based potent sirtuin inhibitor containing Nε-thiocarbamoyl-lysine (TuAcK)

    OpenAIRE

    2011-01-01

    In the current study, we have identified Nε-thiocarbamoyl-lysine (TuAcK) as a general sirtuin inhibitory warhead which was shown to be able to confer potent sirtuin inhibition. This inhibition was also shown to be mechanism-based in that the TuAck residue was able to be processed by a sirtuin enzyme with the formation of a stalled S-alkylamidate intermediate.

  4. Cloning and Characterization of EF-Tu and EF-Ts from Pseudomonas aeruginosa

    Directory of Open Access Journals (Sweden)

    Stephanie O. Palmer

    2013-01-01

    Full Text Available We have cloned genes encoding elongation factors EF-Tu and EF-Ts from Pseudomonas aeruginosa and expressed and purified the proteins to greater than 95% homogeneity. Sequence analysis indicated that P. aeruginosa EF-Tu and EF-Ts are 84% and 55% identical to E. coli counterparts, respectively. P. aeruginosa EF-Tu was active when assayed in GDP exchange assays. Kinetic parameters for the interaction of EF-Tu with GDP in the absence of EF-Ts were observed to be = 33 μM, = 0.003 s−1, and the specificity constant was  s−1 μM−1. In the presence of EF-Ts, these values were shifted to = 2 μM, = 0.005 s−1, and the specificity constant was  s−1 μM−1. The equilibrium dissociation constants governing the binding of EF-Tu to GDP ( were 30–75 nM and to GTP ( were 125–200 nM. EF-Ts stimulated the exchange of GDP by EF-Tu 10-fold. P. aeruginosa EF-Tu was active in forming a ternary complex with GTP and aminoacylated tRNA and was functional in poly(U-dependent binding of Phe-tRNAPhe at the A-site of P. aeruginosa ribosomes. P. aeruginosa EF-Tu was active in poly(U-programmed polyphenylalanine protein synthesis system composed of all P. aeruginosa components.

  5. An Exploration of Grid Computing to be Utilized in Teaching and Research at TU

    Directory of Open Access Journals (Sweden)

    Dr. Mohammed

    2014-11-01

    Full Text Available Taiz University (TU has a hundreds of computing resources on different campuses for use in areas from offices work to general access student labs. However, these resources are not used to their full potential. Grid computing is a technology that is capable to unify these resources and utilize them in very significant way. The difficulties of funding a complete grid computing environment and also, the difficulties of grid tools makes teachers and researchers in TU unable to involve in teaching and research in grid computing or in distributed computing. These problems raised up our awareness to mitigate this problem by build a simple environment for Grid computing from resources are available in TU and the built environment we can use it for teaching and research. The objective of this paper is to build, implement and testing a grid computing environment (Globus Toolkit. To achieving this objective we built the hardware and software parts, and configured several basic grid services commands line and web portal. The test result for basic grid services have been indicated that our proposed grid computing model is promising and can use in teaching and research in TU. The paper takes a look at how grid computing is realizing this aim and have created unbelievable opportunities for students, teachers and researchers at TU in addition the result of this paper will make TU a pilot to the other universities in whole Yemen in field of Grid and distributing computing.

  6. The multi-component Tu hierarchy of soliton equations and its multi-component integrable couplings system

    Institute of Scientific and Technical Information of China (English)

    Xia Tie-Cheng; Wang Hong; Zhang Yu-Feng

    2005-01-01

    A new simple loop algebra GM is constructed, which is devoted to the establishing of an isospectral problem. By making use of the Tu scheme, the multi-component Tu hierarchy is obtained. Furthermore, an expanding loop algebra FM of the loop algebra GM is presented. Based on the FM, the multi-component integrable coupling system of the multi-component Tu hierarchy has been worked out. The method can be applied to other nonlinear evolution equation hierarchies.

  7. Influencia da betametasona, empregada na forma de preparações farmaceuticas distintas, sobre alguns valores de auto-hemostasia, em ratos

    OpenAIRE

    Solange Maria Dieterich Rabelo Junqueira

    1992-01-01

    Resumo: Este trabalho teve por objetivo avaliar os efeitos da betametasona, empregada na forma de duas preparações farmacêuticas distintas, sobre alguns valores de auto-hemostasia. Para tanto,foram utilizados 30 ratos Wistar,machos,divididos em 3 grupos experimentais,tratados (via 1M) com solução de NaCI a 0,9% (Grupo Controle), fosfato dissódico de betametasona ou com a associação deste mesmo sal ao acetato de betametasona, que possibilita uma ação farmacológica de maior duração do glucocor...

  8. Identification and characterization of Tu88, an antigenic gene from Theileria uilenbergi.

    Science.gov (United States)

    Lu, Yizhu; Wang, Yuefeng; Li, Youquan; Gou, Huitian; Luo, Jianxun; Yin, Hong; Liu, Zhijie

    2015-06-01

    Theileria uilenbergi is a pathogen that causes ovine theileriosis. Prevention and control of theileriosis relies on its diagnosis at early stages of occurrence and requires understanding of proteins with antigenic properties from the pathogen. Despite its prevalence in China, only a few molecules with antigenic properties have been characterized from T. uilenbergi. In this study, we identified a cDNA named Tu88 by immunoscreening a T. uilenbergi merozoite cDNA library with T. uilenbergi-positive sera from infected sheep. Recombinant Tu88 (rTu88) expressed in bacteria reacted strongly with the positive sera of T. uilenbergi in western blot analysis indicating its potential as an antigen. Southern blot analysis showed that it is a single copy gene. Protein localization by immunostaining blood smears from an infected sheep demonstrated the presence of native Tu88 in merozoites. These findings suggest that Tu88 is a potential candidate antigen for the development of a sero-diagnostic tool.

  9. Calibration Methods for Air Coupled Antennas - COST Action TU1208

    Science.gov (United States)

    Marecos, Vânia; Solla, Mercedes; Fontul, Simona; Pajewski, Lara

    2016-04-01

    This work focuses on the comparison of different methods for calibrating air coupled antennas: Coring, Surface Reflection Method (SRM) and Common Mid-Point (CMP) through the analysis of GPR data collected in a test site with different pavement solutions. Research activities have been carried out during a Short Term Scientific Mission (STSM) funded by the COST (European Cooperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in December 2015. The use of GPR in transport infrastructures represents one of the most significant advances for obtaining continuous data along the road, with the advantage of operation at traffic speed and being a non-destructive technique. Its main application has been the evaluation of layer thickness. For the determination of layer thickness, it is necessary to know the velocity of the signal, which depends on the dielectric constant of the material, and the two-way travel time of the reflected signal that is recorded by the GPR system. The calculation of the dielectric value of the materials can be done using different approaches such as: using fixed values based on experience, laboratory determination of dielectric values, applying the SRM, performing back calculation from ground truth references such as cores and test pits, or using the CMP method. The problem with using ground truth is that it is time consuming, labour intensive and intrusive to traffic, in addition, a drill core is not necessarily representative of the whole surveyed area. Regarding the surface reflection technique, one of the problems is that it only measures the dielectric value from the layer surface and not from the whole layer. Recent works already started to address some of these challenges proposing new approaches for GPR layer thickness measurements using multiple antennas to calculate the average dielectric value of the asphalt layer, taking advantage of significant hardware improvements in GPR

  10. Preparação e caracterização de membranas cerâmicas tubulares de mulita

    Directory of Open Access Journals (Sweden)

    F. A. Silva

    2013-09-01

    Full Text Available Os produtos cerâmicos são extremamente interessantes no campo das membranas por causa de sua resistência mecânica, inércia química, tempo de vida útil longa e estabilidade térmica. Atualmente as pesquisas estão sendo direcionadas para exploração destes materiais de baixo custo. Este trabalho consiste na preparação de membranas tubulares, obtidas a partir de composição contendo argila, caulim e óxido de alumínio, conformadas pelo processo de extrusão, utilizando diferentes temperaturas na etapa de sinterização para uso em microfiltração. Foi feita caracterização nas massas cerâmicas antes da sinterização, através dos ensaios de análise granulométrica, análise termogravimétrica, análise química e difração de raios X. A análise química mostrou altos teores de SiO2 e de Al2O3 e o ensaio de difração de raios X mostrou a presença de caulinita, quartzo e óxido de alumínio. Foi realizado ensaio de difração de raios X na massa após as sinterizações e os resultados mostraram a presença de mulita, óxido de alumínio e quartzo em todas as sinterizações efetuadas. Com relação ao tamanho e a distribuição destes poros nas membranas, os resultados da micrografia e de porosimetria por intrusão de mercúrio ilustraram a presença de poros em todas as temperaturas de sinterização, com dimensões na faixa de microfiltração. O ensaio de fluxo com escoamento tangencial utilizando água destilada mostrou que a membrana sinterizada a 1300 ºC obteve o maior valor, seguida da de 1250, 1200 e 1150 ºC.

  11. 小猪和小虎——SUZUKI TU250 Volty VS Kawasaki Estrella

    Institute of Scientific and Technical Information of China (English)

    杜启弘; 徐天翔

    2005-01-01

    在日本250毫升复古车中.四大厂家各自推出了自己的代表车型。雅马哈的SRV250.本田的GB250.铃木TU250 Volty、川崎Estrella。其中,GB250大获成功,SRV250则表现平平,TU250 Volty和Estrella则紧紧咬住GB250.在日本国内和国外市场上均取得不错的销量。本期试车我们就将对铃木TU250和川崎的Estrella进行对比测试。

  12. [Analysis on intestinal disorders in Jiujing Tu (Illustration of Moxiustion) found from Dunhuang].

    Science.gov (United States)

    Shi, Yin; Shang, Haixia; Wu, Huangan

    2016-05-01

    Jiujing Tu (Illustration of Moxibustion), excavated from Mo Kao Grotto at Dunhuang, is one of the earliest existing monographs on moxibustion. The medical masters from different schools have focused on this book because it is different from the existing ancient medical works and have not been collected in the medical works of different dynasties. In this study, the literature of Jiujing Tu on five acupoints (Dachangshu, Pangguangshu, Daxiaochangshu, Nieshu and Cigong) relevant with intestinal disorders is collected. It is intended to discuss and analyze the acupoint location, main intestinal disorder, moxibustion characters, recognition on the literature of different dynasties and modern clinical applications. It is believed that the thought of strong moxibustion in the treatment of intestinal disorders advocated in Jiujing Tu has profound impact on the medical development in later generations. It deserves us to have a further digging, collection and promotion of this thought in the modern time.

  13. The tuf3 gene of Streptomyces coelicolor A3(2) encodes an inessential elongation factor Tu that is apparently subject to positive stringent control

    NARCIS (Netherlands)

    Wezel, Gilles P. van; Takano, Eriko; Vijgenboom, Erik; Bosch, Leendert; Bibb, Mervyn J.

    1995-01-01

    In Streptomyces coelicolor A3(2), two genes, tuf1 and tuf3, encode the apparent polypeptide chain elongation factors EF-Tu1 and EF-Tu3, respectively. While tuf1 appears to code for the major EF-Tu, the function of tuf3 is unknown. To assess the role of EF-Tu3, tuf3 was subjected to mutational and

  14. The tuf3 gene of Streptomyces coelicolor A3(2) encodes an inessential elongation factor Tu that is apparently subject to positive stringent control

    NARCIS (Netherlands)

    Wezel, Gilles P. van; Takano, Eriko; Vijgenboom, Erik; Bosch, Leendert; Bibb, Mervyn J.

    1995-01-01

    In Streptomyces coelicolor A3(2), two genes, tuf1 and tuf3, encode the apparent polypeptide chain elongation factors EF-Tu1 and EF-Tu3, respectively. While tuf1 appears to code for the major EF-Tu, the function of tuf3 is unknown. To assess the role of EF-Tu3, tuf3 was subjected to mutational and tr

  15. Towards an understanding of structure-function relationships of elongation factor Tu

    DEFF Research Database (Denmark)

    Wiborg, O; Andersen, C; Knudsen, Charlotte Rohde

    1994-01-01

    In light of the recently determined structure of elongation factor Tu, and taking into account chemical studies mapping functional sites, a number of residues have been selected for site-directed mutagenesis studies. Gly94, Gly126, His66, His118, Lys89 and Asp90 have each been point-mutated. Prel......In light of the recently determined structure of elongation factor Tu, and taking into account chemical studies mapping functional sites, a number of residues have been selected for site-directed mutagenesis studies. Gly94, Gly126, His66, His118, Lys89 and Asp90 have each been point...

  16. A Practical Adaptive TuCM Scheme for Rayleigh Flat Fading Channels

    Institute of Scientific and Technical Information of China (English)

    伍守豪; 宋文涛

    2004-01-01

    A practical adaptive turbo coded modulation (TuCM) scheme was proposed and its adaptive method was described. With some hardware considerations, a suboptimal optimization algorithm that the number of fading regions is variable was put forward. Furthermore, the cutoff fade depth of power adaptation was modified to reduce the interruption probability. The results show that the proposed adaptive TuCM comes within 3 dB of Rayleigh fading channel capacity, and exhibits about 3 dB power gain relative to the conventional adaptive trellis-coded modulation (TCM), and is easy to realize by hardware.

  17. EF-Tu from the enacyloxin producing Frateuria W-315 strain: Structure/activity relationship and antibiotic resistance.

    Science.gov (United States)

    Créchet, Jean-Bernard; Malosse, Christian; Hountondji, Codjo

    2016-08-01

    In this report, we have demonstrated that the poly(U)-dependent poly(Phe) synthesis activity of elongator factor Tu (EF-Tu) from the enacyloxin producing strain Frateuria sp. W-315 is inhibited by the antibiotic similarly to that of Escherichia coli EF-Tu. The inhibitory effect of enacyloxin observed in a purified system was the same as that obtained with an S30 extract from E. coli or Frateuria sp. W-315, respectively, suggesting that antibiotic resistance of enacyloxin producing Frateuria sp. W-315 is not due neither to EF-Tu nor to other components of the translation machinery but to a still unknown mechanism. The EF-Tu gene, as PCR amplified from Frateuria W-315 genomic DNA and sequenced represented an ORF of 1191 nucleotides corresponding to 396 amino acids. This protein is larger than the product of tufA from E. coli by only two amino acid residues. Alignment of the amino acid sequence of EF-Tu from E. coli with those of Frateuria and Ralstonia solanacearum indicates on average 80% identical amino acid residues and 9.7% conservative replacements between EF-Tu Frateuria and EF-Tu E. coli, on one hand, and 97% identity and 1.7% conservative replacement between EF-Tu Frateuria and EF-Tu Ralstonia solanacearum, on the other hand. These strong primary structure similarities between EF-Tu from different origins are consistent with the fact that this factor is essential for the translation process in all kingdoms of life. Comparison of the effects of antibiotics on EF-Tu Frateuria and EF-Tu E. coli revealed that enacyloxin, kirromycin and pulvomycin exert a stronger stimulation of the GDP dissociation rate on EF-Tu Frateuria, while the effects of the antibiotics on the GDP association rate were comparable for the two EF-Tu species. Different mutants of EF-Tu E. coli were constructed with the help of site directed mutagenesis by changing one or several residues of EF-Tu E. coli by the corresponding residues of EF-Tu Frateuria. The single A45K substitution did

  18. "V tu ossen mõ ne nabljudali tshissel..." : [luuletused] / Svetlana Blomberg

    Index Scriptorium Estoniae

    Blomberg, Svetlana

    2000-01-01

    Sisu: "V tu ossen mõ ne nabljudali tshissel..." ; "Tshto delat mne, zalozhnitse zimõ..." ; "Zlaja tshornaja burja na mir naletela..." ; Stirka na beregu zaliva ; Mozhno s proshlõm sporit i srazhatsja ; Mezhdu listjev opavshih kashtan otõskat..."

  19. Forze hydrogen racing team Delft; TU Delft students develop hydrogen race-car

    NARCIS (Netherlands)

    Hartvelt, M.

    2014-01-01

    ‘Working towards a sustainable, yet exciting future’ is one of the big challenges in engineering nowadays. A group of students from the TU Delft accepted this challenge and designed a zero-emission hydrogen powered race-car. Combining green technology with racing, Forze wants to show the potential

  20. Bacterial elongation factors EF-Tu, their mutants, chimeric forms, and domains: isolation and purification.

    Science.gov (United States)

    Jonák, J

    2007-04-15

    Prokaryotic elongation factors EF-Tu form a family of homologous, three-domain molecular switches catalyzing the binding of aminoacyl-tRNAs to ribosomes during the process of mRNA translation. They are GTP-binding proteins, or GTPases. Binding of GTP or GDP regulates their conformation and thus their activity. Because of their particular structure and regulation, various activities (also outside of the translation system) and a relative abundance they represent attractive tools for studies of many basic but still not fully understood mechanisms both of the translation process, the structure-function relationships in EF-Tu molecules themselves and proteins and energy transduction mechanisms in general. The review critically summarizes procedures for the isolation and purification of native and engineered eubacterial elongation factors EF-Tu and their mutants on a large as well as small scale. Current protocols for the purification of both native and polyHis-tagged or glutathione-S-transferase (GST)-tagged EF-Tu proteins and their variants using conventional procedures and the Ni-NTA-Agarose or Glutathione Sepharose are presented.

  1. An efficient algorithm for nucleolus and prekernel computation in some classes of TU-games

    NARCIS (Netherlands)

    Faigle, U.; Kern, W.; Kuipers, J.

    1998-01-01

    We consider classes of TU-games. We show that we can efficiently compute an allocation in the intersection of the prekernel and the least core of the game if we can efficiently compute the minimum excess for any given allocation. In the case where the prekernel of the game contains exactly one core

  2. CWI and TU Delft at TREC 2013: Contextual Suggestion, Federated Web Search, KBA, and Web Tracks

    NARCIS (Netherlands)

    Bellogín Kouki, A.; Gebremeskel, G.G.; He, J.; Lin, J.J.P.; Said, A.; Samar, T.; Vries, A.P. de; Vuurens, J.B.P.

    2014-01-01

    This paper provides an overview of the work done at the Centrum Wiskunde & Informatica (CWI) and Delft University of Technology (TU Delft) for different tracks of TREC 2013. We participated in the Contextual Suggestion Track, the Federated Web Search Track, the Knowledge Base Acceleration (KBA) Trac

  3. RESULTS OF AIRPLANE TU-204-300 OPERATED BY "VLADIVOSTOK AVIA" COMPANY FUEL FLOW MONITIRING

    Directory of Open Access Journals (Sweden)

    G. E. Maslennikova

    2014-01-01

    Full Text Available This article describes the results obtained from continuous monitoring of fuel flow on the airplanes Tu-204-300, operated by the aircompany "Vladivostok Avia". The reasons for the change in the cost of each copy of airplane and changes in fuel characteristics due to pilots manner of flying.

  4. XIA AND THE TU STATE IN THE ORACLE INSCRIPTIONS: A CONSIDERATION

    Institute of Scientific and Technical Information of China (English)

    HuHouxuan

    1995-01-01

    In recent years,the studies on the Xia culture entered a new stage~2.In thepresent article,the writer investigates the identification of the Xia peoplewith the Tu state based on the archaeological evidence,the ancient recordsand the oracle inscriptions of the Shang Dynasty.

  5. HOPE: Helmet Optimization in Europe. The final report of COST Action TU1101.

    NARCIS (Netherlands)

    Bogerd, C.P. Annaheim, S. Halldin, P. Houtenbos, M. Otte, D. Shinar, D. Walker, I. & Willinger, R.

    2015-01-01

    The primary purpose of COST Action TU1101 was to stimulate collaboration and networking amongst European scientists working in the field of bicycle helmet safety and improvement. By gathering together in a single, collective Action, researchers can improve the collection and dissemination of data fr

  6. HOPE: Helmet Optimization in Europe. The final report of COST Action TU1101.

    NARCIS (Netherlands)

    Bogerd, C.P. Annaheim, S. Halldin, P. Houtenbos, M. Otte, D. Shinar, D. Walker, I. & Willinger, R.

    2015-01-01

    The primary purpose of COST Action TU1101 was to stimulate collaboration and networking amongst European scientists working in the field of bicycle helmet safety and improvement. By gathering together in a single, collective Action, researchers can improve the collection and dissemination of data fr

  7. Comparison of Plasma Tu-M2-PK and CA19-9 in Pancreatic Cancer

    DEFF Research Database (Denmark)

    Joergensen, Maiken Thyregod; Heegaard, Niels H H; Schaffalitzky de Muckadell, Ove B

    2009-01-01

    because of suspicion of pancreatic cancer. Of these, 51 patients had their conditions diagnosed as PDAC, whereas this diagnosis was ruled out in 52 after 12 months of follow-up. The performance of Tu-M2-PK was compared with that of CA19-9 using cutoff values 15 and 37 U/mL, respectively. RESULTS...

  8. Mapping Escherichia coli elongation factor Tu residues involved in binding of aminoacyl-tRNA

    DEFF Research Database (Denmark)

    Wiborg, Ove; Andersen, C; Knudsen, Charlotte Rohde

    1996-01-01

    were characterized with respect to thermal and chemical stability, GTPase activity, tRNA affinity, and activity in an in vitro translation assay. Most conspicuously tRNA affinities were reduced for all mutants. The results verify our structural analysis of elongation factor Tu in complex with aminoacyl...

  9. α1-Tubulin FaTuA1 plays crucial roles in vegetative growth and conidiation in Fusarium asiaticum.

    Science.gov (United States)

    Hu, Weiqun; Zhang, Xiaoping; Chen, Xiang; Zheng, Jingwu; Yin, Yanni; Ma, Zhonghua

    2015-04-01

    The filamentous ascomycete Fusarium asiaticum contains two homologous genes FaTUA1 and FaTUA2 encoding α-tubulins. In this study, we found that FaTUA2 was dispensable for vegetative growth and sporulation in F. asiaticum. The deletion of FaTUA1 however led to dramatically reduced mycelial growth, twisted hyphae and abnormal nuclei in apical cells of hyphae. The FaTUA1 deletion mutant (ΔFaTuA1-5) also showed a significant decrease in conidiation, and produced abnormal conidia. Pathogenicity assays showed that ΔFaTuA1-5 exhibited decreased virulence on wheat head. Unexpectedly, the deletion of FaTUA1 led to resistance to high temperatures. In addition, ΔFaTuA2 showed increased sensitivity to carbendazim. Furthermore, increased FaTUA2 expression in ΔFaTuA1-5 partially restored the defects of the mutant in mycelial growth, conidial production and virulence, vice versa, increased FaTUA1 expression in the FaTUA2 deletion mutant also partially relieved the defect of the mutant in the delay of conidial germination. Taken together, these results indicate that FaTuA1 plays crucial roles in vegetative growth and development, and the functions of FaTuA1 and FaTuA2 are partially interchangeable in F. asiaticum.

  10. Human oxygen sensing may have origins in prokaryotic elongation factor Tu prolyl-hydroxylation

    Science.gov (United States)

    Scotti, John S.; Leung, Ivanhoe K. H.; Ge, Wei; Bentley, Michael A.; Paps, Jordi; Kramer, Holger B.; Lee, Joongoo; Aik, WeiShen; Choi, Hwanho; Paulsen, Steinar M.; Bowman, Lesley A. H.; Loik, Nikita D.; Horita, Shoichiro; Ho, Chia-hua; Kershaw, Nadia J.; Tang, Christoph M.; Claridge, Timothy D. W.; Preston, Gail M.; McDonough, Michael A.; Schofield, Christopher J.

    2014-01-01

    The roles of 2-oxoglutarate (2OG)-dependent prolyl-hydroxylases in eukaryotes include collagen stabilization, hypoxia sensing, and translational regulation. The hypoxia-inducible factor (HIF) sensing system is conserved in animals, but not in other organisms. However, bioinformatics imply that 2OG-dependent prolyl-hydroxylases (PHDs) homologous to those acting as sensing components for the HIF system in animals occur in prokaryotes. We report cellular, biochemical, and crystallographic analyses revealing that Pseudomonas prolyl-hydroxylase domain containing protein (PPHD) contain a 2OG oxygenase related in structure and function to the animal PHDs. A Pseudomonas aeruginosa PPHD knockout mutant displays impaired growth in the presence of iron chelators and increased production of the virulence factor pyocyanin. We identify elongation factor Tu (EF-Tu) as a PPHD substrate, which undergoes prolyl-4-hydroxylation on its switch I loop. A crystal structure of PPHD reveals striking similarity to human PHD2 and a Chlamydomonas reinhardtii prolyl-4-hydroxylase. A crystal structure of PPHD complexed with intact EF-Tu reveals that major conformational changes occur in both PPHD and EF-Tu, including a >20-Å movement of the EF-Tu switch I loop. Comparison of the PPHD structures with those of HIF and collagen PHDs reveals conservation in substrate recognition despite diverse biological roles and origins. The observed changes will be useful in designing new types of 2OG oxygenase inhibitors based on various conformational states, rather than active site iron chelators, which make up most reported 2OG oxygenase inhibitors. Structurally informed phylogenetic analyses suggest that the role of prolyl-hydroxylation in human hypoxia sensing has ancient origins. PMID:25197067

  11. Human oxygen sensing may have origins in prokaryotic elongation factor Tu prolyl-hydroxylation.

    Science.gov (United States)

    Scotti, John S; Leung, Ivanhoe K H; Ge, Wei; Bentley, Michael A; Paps, Jordi; Kramer, Holger B; Lee, Joongoo; Aik, WeiShen; Choi, Hwanho; Paulsen, Steinar M; Bowman, Lesley A H; Loik, Nikita D; Horita, Shoichiro; Ho, Chia-hua; Kershaw, Nadia J; Tang, Christoph M; Claridge, Timothy D W; Preston, Gail M; McDonough, Michael A; Schofield, Christopher J

    2014-09-16

    The roles of 2-oxoglutarate (2OG)-dependent prolyl-hydroxylases in eukaryotes include collagen stabilization, hypoxia sensing, and translational regulation. The hypoxia-inducible factor (HIF) sensing system is conserved in animals, but not in other organisms. However, bioinformatics imply that 2OG-dependent prolyl-hydroxylases (PHDs) homologous to those acting as sensing components for the HIF system in animals occur in prokaryotes. We report cellular, biochemical, and crystallographic analyses revealing that Pseudomonas prolyl-hydroxylase domain containing protein (PPHD) contain a 2OG oxygenase related in structure and function to the animal PHDs. A Pseudomonas aeruginosa PPHD knockout mutant displays impaired growth in the presence of iron chelators and increased production of the virulence factor pyocyanin. We identify elongation factor Tu (EF-Tu) as a PPHD substrate, which undergoes prolyl-4-hydroxylation on its switch I loop. A crystal structure of PPHD reveals striking similarity to human PHD2 and a Chlamydomonas reinhardtii prolyl-4-hydroxylase. A crystal structure of PPHD complexed with intact EF-Tu reveals that major conformational changes occur in both PPHD and EF-Tu, including a >20-Å movement of the EF-Tu switch I loop. Comparison of the PPHD structures with those of HIF and collagen PHDs reveals conservation in substrate recognition despite diverse biological roles and origins. The observed changes will be useful in designing new types of 2OG oxygenase inhibitors based on various conformational states, rather than active site iron chelators, which make up most reported 2OG oxygenase inhibitors. Structurally informed phylogenetic analyses suggest that the role of prolyl-hydroxylation in human hypoxia sensing has ancient origins.

  12. UtilizaÃÃo da Ãgua de coco em pà na preparaÃÃo de nanopartÃculas de ferritas

    OpenAIRE

    Nizomar de Sousa GonÃalves

    2007-01-01

    Este trabalho à dedicado aos estudos da preparaÃÃo e caracterizaÃÃo das ferritas de nÃquel e cobalto. Estas ferritas foram sintetizadas pelo processo de sol-gel em suspensÃo de Ãgua de coco liofilizada (ACP) fabricada no CearÃ. Este mÃtodo de sÃntese permitiu obter de forma eficiente nanopartÃculas de ferritas. As amostras foram calcinadas durante 4 horas a 400, 600, 800, 1000 e 1200 ÂC. Um estudo preliminar da ferrita de cobalto variando a concentraÃÃo da Ãgua de coco acima da sua concentraÃ...

  13. Preparação para a aposentadoria: implantação e avaliação do programa viva mais!

    Directory of Open Access Journals (Sweden)

    Sheila Giardini Murta

    2014-03-01

    Full Text Available O objetivo deste artigo é descrever o processo de implementação e avaliação do Viva Mais! - o programa de preparação para aposentadoria (PPA de uma Universidade pública brasileira. Para tal, utilizou-se um modelo lógico baseado em insumos, produtos, resultados, pressupostos e fatores externos. Treze participantes inscritos foram divididos em dois grupos que receberam 24 horas de intervenção informativa e vivencial durante oito semanas. Os temas basearam-se nos preditores do envelhecimento bem-sucedido. Utilizou-se a análise de conteúdo para interpretar as respostas dos participantes nas entrevistas de avaliação de resultados. Os dados demonstraram que o principal objetivo do programa - promover o bem-estar, a saúde e a qualidade de vida dos trabalhadores da Universidade - foi alcançado.

  14. Papel de EF-Tu en la interacción de Pseudomonas aeruginosa con los queratinocitos humanos

    OpenAIRE

    Martínez Ramos, Inmaculada

    2015-01-01

    Introducción: Pseudomonas aeruginosa es un patógeno oportunista que causa una gran variedad de infecciones, entre las que destacan las infecciones respiratorias agudas y crónicas y las infecciones de heridas por quemaduras. En un trabajo previo, usamos anticuerpos específicos para detectar la presencia del epítopo de fosforilcolina en la superficie bacteriana asociado al factor de elongación Tu (EF-Tu). Identificamos que el gen eftM (Elongation Factor Tu Modifying enzime) es el...

  15. Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation

    National Research Council Canada - National Science Library

    Choudhury, Nila Roy; Nowak, Jakub S; Zuo, Juan; Rappsilber, Juri; Spoel, Steven H; Michlewski, Gracjan

    2014-01-01

    .... Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated pre-let-7 uridylation, inhibiting its processing and targeting it for degradation...

  16. A neutron scattering study of the ternary complex EF-Tu.GTP-valyl-tRNAVal1A

    DEFF Research Database (Denmark)

    Österberg, R.; Elias, P.; Kjems, Jørgen

    1986-01-01

    matched by the solvents. The results indicate that EF-Tu undergoes a conformational change and contracts as a result of the complex formation, since the radius of gyration decreases by 15% from 2.82 to 2.39 nm. tRNAVal1A, on the other hand, seems to mainly retain its conformation within the complex, since......The complex formation between elongation factor Tu (EF-Tu), GTP, and valyl-tRNAVal1A has been investigated in a hepes buffer of "pH" 7.4 and 0.2 M ionic strength using the small-angle neutron scattering method at concentrations of D2O where EF-Tu (42% D2O) and tRNA (71% D2O) are successively...

  17. Identification and purification of human erythroid progenitor cells by monoclonal antibody to the transferrin receptor (TU 67).

    Science.gov (United States)

    Herrmann, F; Griffin, J D; Sabbath, K D; Oster, W; Wernet, P; Mertelsmann, R

    1988-04-01

    Anti-TU 67 is a murine monoclonal antibody that recognizes the transferrin receptor. With respect to hematopoietic cells TU 67 is expressed by human multipotent colony-forming cells (CFU-Mix), erythroid progenitor cells (BFU-E and CFU-E) and a fraction of granulocyte/monocyte colony forming cells, but is not expressed by mature hematopoietic cells including erythrocytes, platelets, lymphocytes, and peripheral blood myeloid cells. The TU 67-positive fraction of normal bone marrow, separated by fluorescence-activated cell sorting (FACS) or immune rosettes, contained 87% of the erythroid progenitor cells. Erythroid progenitor cells were enriched up to 50-fold by using a combination of monoclonal antibodies to deplete mature hematopoietic cells, followed by positive selection of BFU-E and CFU-E by TU 67 antibody.

  18. Function and structure in phage Qbeta RNA replicase. Association of EF-Tu-Ts with the other enzyme subunits

    DEFF Research Database (Denmark)

    Blumenthal, T; Young, R A; Brown, S

    1976-01-01

    Qbeta replicase is a complex of four nonidentical subunits readily dissociable into two subcomplexes: 30 S ribosomal protein S1 and the phage-coded polypeptide (Subunits I + II) and protein synthesis elongation factors EF-Tu and EF-Ts (Subunits III + IV). The affinity of the two subcomplexes...... of EF-Tu-Ts from the other enzyme subunits: whereas free EF-Tu-Ts binds GDP independently of salt concentration, this binding by Qbeta replicase is sensitive to high ionic strength and remains so in the presence of Qbeta RNA. Furthermore, RNA does not allow the release of EF-Ts from EF-Tu by GTP...... as measured by sensitivity of EF-Ts activity to N-ethylmaleimide....

  19. A tRNA body with high affinity for EF-Tu hastens ribosomal incorporation of unnatural amino acids.

    Science.gov (United States)

    Ieong, Ka-Weng; Pavlov, Michael Y; Kwiatkowski, Marek; Ehrenberg, Måns; Forster, Anthony C

    2014-05-01

    There is evidence that tRNA bodies have evolved to reduce differences between aminoacyl-tRNAs in their affinity to EF-Tu. Here, we study the kinetics of incorporation of L-amino acids (AAs) Phe, Ala allyl-glycine (aG), methyl-serine (mS), and biotinyl-lysine (bK) using a tRNA(Ala)-based body (tRNA(AlaB)) with a high affinity for EF-Tu. Results are compared with previous data on the kinetics of incorporation of the same AAs using a tRNA(PheB) body with a comparatively low affinity for EF-Tu. All incorporations exhibited fast and slow phases, reflecting the equilibrium fraction of AA-tRNA in active ternary complex with EF-Tu:GTP before the incorporation reaction. Increasing the concentration of EF-Tu increased the amplitude of the fast phase and left its rate unaltered. This allowed estimation of the affinity of each AA-tRNA to EF-Tu:GTP during translation, showing about a 10-fold higher EF-Tu affinity for AA-tRNAs formed from the tRNA(AlaB) body than from the tRNA(PheB) body. At ∼1 µM EF-Tu, tRNA(AlaB) conferred considerably faster incorporation kinetics than tRNA(PheB), especially in the case of the bulky bK. In contrast, the swap to the tRNA(AlaB) body did not increase the fast phase fraction of N-methyl-Phe incorporation, suggesting that the slow incorporation of N-methyl-Phe had a different cause than low EF-Tu:GTP affinity. The total time for AA-tRNA release from EF-Tu:GDP, accommodation, and peptidyl transfer on the ribosome was similar for the tRNA(AlaB) and tRNA(PheB) bodies. We conclude that a tRNA body with high EF-Tu affinity can greatly improve incorporation of unnatural AAs in a potentially generalizable manner.

  20. Genetic polymorphism of mitochondrial DNA HVS-I and HVS-Ⅱ of Chinese Tu ethnic minority group

    Institute of Scientific and Technical Information of China (English)

    Feng Chen; Yajun Deng; Yonghui Dang; BO Zhang; Haofang Mu; Xiaoguang Yu; Lin Li; Chunxia Yan; Teng Chen

    2008-01-01

    We analyzed the two hypervariable segments HVS-Ⅰ and HVS-Ⅱ of 108 Chinese Tu ethnic minority group samples for forensic and population genetics purposes.Comparing with Anderson sequence,79 polymorphic loci in HVS-Ⅰ and 40 in HVS-Ⅱ were found in Chi-nese Tu ethnic minority group mtDNA sequences,and 90 and 64 haplotypes were then defined.Haplotype diversity and the mean pair-wise differences were 0.9903:±0.0013 and 5.7785 in HVS-Ⅰ,and 0.9777±0.0013 and 3.5819 in HVS-Ⅱ,respectively.By analyzing the hypervariable domain from nucleotide 1,6180 to 1,6193 in HVS-Ⅰ,we defined some new types of sequence variations.We also compared the relationship between Tu population and other populations using mtDNA HVS-Ⅰ sequences.According to Rst genetic distances,the phylogenetic tree showed that the Tu population,the Xi'an Han population,the Chinese Korean,and the Mongol ethnic group were in a clade.This indicated a close genetic relationship between them.There were far relations between the Tu population and other Chinese southern Han populations,Siberian,European,African,and other foreign populations.The results suggest that Tu population has a multi-origin and has also merged with other local populations.

  1. Forze hydrogen racing team Delft; TU Delft students develop hydrogen race-car

    OpenAIRE

    Hartvelt, M.

    2014-01-01

    ‘Working towards a sustainable, yet exciting future’ is one of the big challenges in engineering nowadays. A group of students from the TU Delft accepted this challenge and designed a zero-emission hydrogen powered race-car. Combining green technology with racing, Forze wants to show the potential of hydrogen as an alternative fuel. Find out how Forze has been developing hydrogen powered racecars since 2007.

  2. Töötu laevamehhaanik õppis seitsme kuuga kokaks / Agne Narusk

    Index Scriptorium Estoniae

    Narusk, Agne, 1971-

    2004-01-01

    Töötu Jevgeni Solovjov õppis OÜ-s Isabella kokaks, ettevõte on üks 54 koolitajast, kellelt Tallinna tööhõiveamet teenust ostab. 2004. aastaks oli Tallinna tööhõiveametil tööotsijate koolitamiseks 7 mln krooni, millest jätkus pooleks aastaks. Lisa: Tööandjad saavad küsitluslehed

  3. Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues.

    Science.gov (United States)

    Sato, Aya; Suematsu, Takuma; Aihara, Koh-Ki; Kita, Kiyoshi; Suzuki, Tsutomu; Watanabe, Kimitsuna; Ohtsuki, Takashi; Watanabe, Yoh-Ichi

    2017-03-07

    Translation elongation factor Tu (EF-Tu) delivers aminoacyl-tRNA (aa-tRNA) to ribosomes in protein synthesis. EF-Tu generally recognizes aminoacyl moieties and acceptor- and T-stems of aa-tRNAs. However, nematode mitochondrial (mt) tRNAs frequently lack all or part of the T-arm that is recognized by canonical EF-Tu. We previously reported that two distinct EF-Tu species, EF-Tu1 and EF-Tu2, respectively, recognize mt tRNAs lacking T-arms and D-arms in the mitochondria of the chromadorean nematode Caenorhabditis elegansC. elegans EF-Tu2 specifically recognizes the seryl moiety of serylated D-armless tRNAs. Mitochondria of the enoplean nematode Trichinella possess three structural types of tRNAs: T-armless tRNAs, D-armless tRNAs, and cloverleaf tRNAs with a short T-arm. Trichinella mt EF-Tu1 binds to all three types and EF-Tu2 binds only to D-armless Ser-tRNAs, showing an evolutionary intermediate state from canonical EF-Tu to chromadorean nematode (e.g. C. elegans) EF-Tu species. We report here that two EF-Tu species also participate in Drosophila melanogaster mitochondria. Both D. melanogaster EF-Tu1 and EF-Tu2 bound to cloverleaf and D-armless tRNAs. D. melanogaster EF-Tu1 has the ability to recognize T-armless tRNAs that do not evidently exist in D. melanogaster mitochondria, but do exist in related arthropod species. In addition, D. melanogaster EF-Tu2 preferentially bound to aa-tRNAs carrying small amino acids, but not to aa-tRNAs carrying bulky amino acids. These results suggest that the Drosophila mt translation system could be another intermediate state between the canonical and nematode mitochondria-type translation systems. © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society.

  4. Designing of Ground Truth Annotated DBT-TU-JU Breast Thermogram Database towards Early Abnormality Prediction.

    Science.gov (United States)

    Bhowmik, Mrinal Kanti; Gogoi, Usha Rani; Majumdar, Gautam; Bhattacharjee, Debotosh; Datta, Dhritiman; Ghosh, Anjan Kumar

    2017-08-17

    The advancement of research in a specific area of clinical diagnosis crucially depends on the availability and quality of the radiology and other test related databases accompanied by ground truth and additional necessary medical findings. The paper describes the creation of the Department of Biotechnology-Tripura University-Jadavpur University (DBT-TU-JU) breast thermogram database. The objective of creating the DBT-TU-JU database is to provide a breast thermogram database that is annotated with the ground truth images of the suspicious regions. Along with the result of breast thermography, the database comprises of the results of other breast imaging methodologies. A standard breast thermogram acquisition protocol suite comprising of several critical factors has been designed for the collection of breast thermograms. Currently, the DBT-TU-JU database contains 1100 breast thermograms of 100 subjects. Due to the necessity of evaluating any breast abnormality detection system, this study emphasizes the generation of the ground truth images of the hotspot areas, whose presence in a breast thermogram signifies the presence of breast abnormality. With the generated ground truth images, we compared the results of six state-of-the-art image segmentation methods using five supervised evaluation metrics to identify the proficient segmentation methods for hotspot extraction. Based on the evaluation results, the Fractional-Order Darwinian particle swarm optimization, Region growing, Mean shift and Fuzzy c-means clustering are found to be more efficient in comparison to k-means clustering and Threshold based segmentation methods.

  5. Revisão sistemática sobre antissepsia cirúrgica das mãos com preparação alcoólica em comparação aos produtos tradicionais

    Directory of Open Access Journals (Sweden)

    Karen de Jesus Gonçalves

    2012-12-01

    Full Text Available A antissepsia cirúrgica das mãos visa à prevenção de infecções do sítio cirúrgico, importante causa de morbimortalidade pós-operatória e aumento dos custos hospitalares. Este estudo teve como objetivo comparar a eficácia de preparações alcoólicas com os produtos tradicionais na antissepsia cirúrgica das mãos por meio de uma revisão sistemática da literatura. Foram considerados estudos primários ou secundários, tendo como desfecho a contagem microbiana das mãos ou taxas de infecções do sítio cirúrgico. A busca foi realizada no Portal BVS, PubMed, Ask e MEDLINE. Foram selecionados 25 estudos (2 revisões sistemáticas, 19 experimentais e 4 de coorte. As preparações alcoólicas tiveram uma redução microbiana igual e/ou maior aos produtos tradicionais em 17 estudos e inferior em 4; as taxas de infecções do sítio cirúrgico foram similares. Portanto, existem evidências científicas que suportam a segurança das preparações alcoólicas para antissepsia cirúrgica das mãos.

  6. Research data from hydrology disciplines are safely stored and published in 3TU.Datacentrum

    Science.gov (United States)

    Prinčič, Alenka; Rombouts, Jeroen

    2013-04-01

    Good management of research data from different science disciplines such as hydrology, geodesy and climate research, is crucial for reducing the uncertainties in forecasting and predictions. These research data can be well-managed and safely stored in 3TU.Datacentrum and thus become available for future use by the scientific community. 3TU.Datacentrum (datacentrum.3tu.nl) is a portal for science- and technology research data in the Netherlands. It provides long-term archive and permanent access to research data, it provides tools for citation of research datasets and thus enables linking, sharing and reuse of research data. Versatility of disciplines and thus versatility of the data producers' needs is a challenge of the data center. 3TU.Datacentrum offers solutions - as standardized as possible yet different where required. Standardized solution is the data model: datasets, instruments, locations and time frames are all objects on their own, with own metadata and interconnected through rdf relationships. These relations are also used to generate ORE Resource Maps. The file format NetCDF, which is primarily used for sets of multidimensional arrays with metadata included, enables interoperability. For simple datasets a Fedora repository is used where datasets of any format are allowed including that of a specific instrument. Alongside the NetCDF an xml version (ncml) is stored that can be converted to other formats. For complex datasets an OPeNDAP framework is used, which allows querying within and across datasets. Some examples from hydrological research are presented. 1 - For a complex collection of simple datasets consisting of ongoing hydrological measurements from several project partners, using several instruments, on several locations, and over a long period of time, there was a need for central storage in order to share the data and enable easy combination of data sets. The measurements are not repeatable and are also valuable for multidisciplinary research

  7. Efeito das condições de preparação e sinterização sobre a porosidade da hidroxiapatita

    Directory of Open Access Journals (Sweden)

    ROSA Adalberto Luiz

    2000-01-01

    Full Text Available Este estudo foi realizado para verificar o efeito das condições de preparação e sinterização sobre a porosidade de amostras de hidroxiapatita, calculada como porcentagem da densidade teórica da hidroxiapatita. Foram verificados o efeito da carga utilizada para obter as amostras, o tamanho inicial das partículas de hidroxiapatita, a temperatura e o tempo de sinterização. Existiu uma correlação inversa entre carga e porosidade e entre temperatura de sinterização e porosidade, mas nenhuma correlação entre tempo de sinterização e porosidade. Amostras fabricadas com partículas de tamanho maior apresentaram maior porosidade. Foi concluído que para a fabricação de amostras de hidroxiapatita, a combinação entre a carga utilizada para a compactação das partículas e a temperatura de sinterização resulta na produção de amostras com uma ampla variação de porcentagens de porosidade.

  8. The computer science institute building of TU Brunswick University. Construction of an energy-efficient university building; Das Informatikzentrum der TU Braunschweig. Realisierung eines energieeffizienten Institutsgebaeudes

    Energy Technology Data Exchange (ETDEWEB)

    Rozynski, M.; Gerder, F. [Technische Univ. Braunschweig (Germany). Inst. fuer Gebaeude- und Solartechnik

    2003-07-01

    Saving of resources will be a key issue in future building construction. A new building projected on the campus of Brunswick University will have a power supply and ventilation concept that ensures low energy consumption. The project is carried out with funds provided by the Federal Minister of Economics and Technology (BMWi) in the context of the SolarBau funding concept. Construction of the building will be followed by an extensive monitoring programme that is to ensure its perfect function. [German] Eine wesentliche Zielsetzung zukuenftigen Bauens ist der sparsame Umgang mit Ressourcen. Im Rahmen eines integralen Planungsprozesses konnte fuer den Neubau des Informatikzentrums der TU Braunschweig ein Energie- und Lueftungskonzept realisiert werden, dass auf einen niedrigen Energieverbrauch zielt. Das Projekt wird im Rahmen des Foerderkonzeptes SolarBau durch das Bundesministerium fuer Wirtschaft und Technologie (BMWi) gefoerdert. Durch das anschliessende umfangreiche Monitoringprogramm wird derzeit die Funktionsfaehigkeit dieses Konzeptes ueberprueft. (orig.)

  9. Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR

    Institute of Scientific and Technical Information of China (English)

    Fen Lu; Huiqin Wang; Shanzhi Wang; Wendi Jiang; Changlin Shan; Bin Li; Jun Yang; Shiyong Zhang; Wenxian Sun

    2015-01-01

    The elongation factor Tu (EF-Tu) receptor (EFR) in cruciferous plants specifical y recognizes the N-terminal acetylated elf18 region of bacterial EF-Tu and thereby activates plant immunity. It has been demonstrated that Arabidopsis EFR confers broad-spectrum bacterial resistance in the EFR transgenic solanaceous plants. Here, the transgenic rice plants (Oryza sativa L. ssp. japonica cv. Zhonghua 17) and cel cultures with constitutive expression of AtEFR were developed to investigate whether AtEFR senses EF-Tu and thus enhances bacterial resistance in the monocot plants. We demonstrated that the Xanthomonas oryzae-derived elf18 peptide induced oxidative burst and mitogen-activated protein kinase activa-tion in the AtEFR transgenic rice cel s and plants, respectively. Pathogenesis-related genes, such as OsPBZ1, were upregulated dramatical y in transgenic rice plant and cel lines in response to elf18 stimulation. Importantly, pretreatment with elf18 trig-gered strong resistance to X. oryzae pv. oryzae in the transgenic plants, which was largely dependent on the AtEFR expression level. These plants also exhibited enhanced resistance to rice bacterial brown stripe, but not to rice fungal blast. Col ectively, the results indicate that the rice plants with heterologous expression of AtEFR recognize bacterial EF-Tu and exhibit enhanced broad-spectrum bacterial disease resistance and that pattern recognition receptor-mediated immunity may be manipulated across the two plant classes, dicots and monocots.

  10. Identification of Molecular Markers Linked to TuMV Resistant Gene in Cabbage

    Institute of Scientific and Technical Information of China (English)

    GAO Jinping; WANG Chao; LIU Ying

    2008-01-01

    A total of 144 F2 individuals were obtained from the crossing between 1047 (susceptible) and A21 (resistant).Two RAPD markers were screened out in 200 random primers using BSA(Bulked Segregant Analysis). Two RAPD markers, designated as AG13/2000 and U16/660,were 7.7 cM and 8.38 cM apart from the TuMV resistant gene,respectively. The two RAPD fragments were converted to SCAR markers.SCAR markers were confined in germplasm.

  11. Jornada "Cómo crear tu propia asesoría fiscal"

    Directory of Open Access Journals (Sweden)

    Ana María Delgado García

    2016-02-01

    Full Text Available Los Estudios de Derecho y Ciencia Política de la Universitat Oberta de Catalunya organizaron el día 23 de septiembre de 2015 la jornada «Cómo crear
    tu propia asesoría fiscal», bajo la dirección de Ana María Delgado García (Directora del Máster Universitario de Fiscalidad de la UOC y Rafael Oliver Cuello (Abogado, asesor fiscal y profesor consultor de Fiscalidad de la UOC.

  12. Opšte vibracije na sedištu pilota kod dva tipa helikoptera

    OpenAIRE

    Živković Dejan; Basarić Gradimir; Jovelić Stojan

    2002-01-01

    U ovom radu opisano je merenje opštih vibracija na sedištu pilota kod dva tipa helikoptera (po jedna letelica) sa turbomlaznim motorima, kao i trećinsko-oktavna analiza ubrzanja opštih vibracija u sve tri ose. Iz dobijenih rezultata sledi da su nivoi ubrzanja kod prvog tipa helikoptera najveći u trećinsko-oktavnom opsegu sa centralnom frekvencijom od 16 Hz. Maksimalne vrednosti ubrzanja opštih vertikalnih vibracija izmerene su za režime lebdenja (0,977 m/s2) i horizontalnog leta pri brzini od...

  13. Ciuda tu corazón, es para toda la vida -Para Disfrutar la Música Clasica

    OpenAIRE

    2012-01-01

    Cuida tu corazón, es para toda la vida. Importantelibro de información sobre prevenciónde de las enfermedades cardiovasculares,dirigido a la comunidad en general.Hace parte de la colección de libros sobre prevención primaria: “Cuidatu corazón, vive mejor” que desde hace varios años viene publicandoel profesor, doctor Fernando Manzur Jattin, desde su grupo de investigación:“Centro de Investigaciones Biomédicas”, perteneciente a laFacultad de Medicina de la Universidad de Cartagena y Categoriza...

  14. Transportvaneundersøgelsen - Variabeldeklaration : TU 2006-09, version 2

    DEFF Research Database (Denmark)

    Christiansen, Hjalmar

    , eller anden årsag. Undervejs omtales en række vigtige detaljer ved spørgeskemaet, men dette notat har ikke til formål at give en udtømmende beskrivelse af hvilke spørgsmål, der er stillet til hvem og hvornår. Her kan det være til stor nytte at besøge testspørgeskemaet på http://tu2010.dk/dev/starttest.php...

  15. Tööotsija töötuks tunnistamise ning töötu abiraha määramise ja maksmise ajutise juhendi kinnitamise kohta

    Index Scriptorium Estoniae

    1991-01-01

    Kehtib 1. aprillist 1991. Lisatud ajutine juhend: töö vahendamine tööotsijale, töötule, töötu abiraha määramine ja maksmine Lisatud: töötu abiraha arvutamine. Muudatus: RT I 1992 nr. 27 art. 362

  16. Site-directed mutagenesis of Arg58 and Asp86 of elongation factor Tu from Escherichia coli: effects on the GTPase reaction and aminoacyl-tRNA binding

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.

    1996-01-01

    Elongation factor Tu from Escherichia coli was mutated separately at positions Asp86 and Arg58, in order to shed light both on the GTPase mechanism of elongation factor Tu and on the binding of aminoacyl-tRNA. In addition, the binding of guanine nucleotides was investigated by determination...

  17. Protein synthesis alongation factors EF-Tu and eEF1A: biosynthesis, functions and application in the improvement of heat tolerance in plants

    Science.gov (United States)

    Protein synthesis elongation factors EF-Tu and eEF1A (EFs) represent a group of highly conserved and abundant GTPases with an important role in transporting the aminoacyl-tRNA complex to the A site of the ribosome during elongation phase of translation. EF-Tu proteins are located in bacteria and, du...

  18. Site-directed mutagenesis of Arg58 and Asp86 of elongation factor Tu from Escherichia coli: effects on the GTPase reaction and aminoacyl-tRNA binding

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Clark, Brian F. C.

    1996-01-01

    Elongation factor Tu from Escherichia coli was mutated separately at positions Asp86 and Arg58, in order to shed light both on the GTPase mechanism of elongation factor Tu and on the binding of aminoacyl-tRNA. In addition, the binding of guanine nucleotides was investigated by determination of th...

  19. Diasystème et spécification de traits en tu'un savi (mixtecan otomangue oriental)

    OpenAIRE

    Léonard, Jean,

    2010-01-01

    This contribution aims to focus on various descriptions and theories of the segmental inventory and distributional constraints of the phonology of Tu'un Savi (Mixtec). The Oto-Manguean languages are reputed to be among the most complex of Meso-America due to the intricacy of their surface forms. Mixtec (known as Tu'un Savi or Ñuu Savi by its speakers) is part of the Mixtecan sub-family, which includes Trique, Cuicatec and probably also Amuzgo (Longacre, 1957, 1961; Longacre & Millon, 1961), b...

  20. Preparação do sistema Fe2O3/ZSM-5 para uso como catalisador na reação foto-Fenton

    Directory of Open Access Journals (Sweden)

    J. S. de Oliveira

    Full Text Available Resumo Este trabalho objetivou a preparação da zeólita ZSM-5 suportada com nanopartículas de óxido de ferro para uso como catalisador na degradação de um poluente orgânico em solução aquosa a partir do processo foto-Fenton. A zeólita foi preparada usando gel nucleante como indutor de formação da estrutura tipo MFI. Nanopartículas de óxido de ferro foram suportadas sobre a zeólita através da técnica de impregnação incipiente. Além disso, nanopartículas de óxido de ferro foram preparadas para fins de comparação entre as atividades catalíticas na reação foto-Fenton. Os materiais produzidos foram caracterizados por difração de raios X, microscopia eletrônica de varredura e análise de adsorção/dessorção de nitrogênio. Os resultados revelaram a formação do sistema Fe2O3/ZSM-5 com propriedades intrínsecas que resultaram em satisfatória atividade catalítica, sendo superior ao óxido de ferro. Além disso, esse sistema apresentou excelente atividade e estabilidade após três ciclos de reuso. O material Fe2O3/ZSM-5 produzido neste trabalho apresenta-se como um catalisador promissor para uso na reação foto-Fenton para a degradação de poluentes orgânicos em soluções aquosas.

  1. Preparação, caracterização e aplicação de membranas anisotrópicas de poliacrilonitrila

    Directory of Open Access Journals (Sweden)

    Lavall Rodrigo C.

    1999-01-01

    Full Text Available A pervaporação, que é um dos processos com membranas empregado em separações seletivas específicas, como misturas azeotrópicas, misturas diluídas e misturas orgânicas, tem despertado um grande interesse nos últimos anos. Neste trabalho foram estudadas a influência de parâmetros, tais como concentração da solução polimérica, tempo de exposição, concentração de aditivo e temperatura de tratamento térmico na preparação de membranas assimétricas de poliacrilonitrila. Em função destes parâmetros, as membranas foram observadas por microscopia eletrônica de varredura (MEV, e determinada a cinética de precipitação em experimentos de transmissão de luz. Os resultados mostram que a precipitação é instantânea. As membranas compostas de poliacrilonitrila, obtidas pelo espalhamento de uma solução diluída de poli(álcool vinílico sobre as membranas assimétricas de poliacrilonitrila, foram caracterizadas na separação de misturas de água/etanol por pervaporação. Os resultados foram comparados com a membrana comercial da GFT de poli(álcool vinílico/poliacrilonitrila.

  2. Tuberculate fruit gene Tu encodes a C2 H2 zinc finger protein that is required for the warty fruit phenotype in cucumber (Cucumis sativus L.).

    Science.gov (United States)

    Yang, Xuqin; Zhang, Weiwei; He, Huanle; Nie, Jingtao; Bie, Beibei; Zhao, Junlong; Ren, Guoliang; Li, Yue; Zhang, Dabing; Pan, Junsong; Cai, Run

    2014-06-01

    Cucumber fruits that have tubercules and spines (trichomes) are known to possess a warty (Wty) phenotype. In this study, the tuberculate fruit gene Tu was identified by map-based cloning, and was found to encode a transcription factor (TF) with a single C2 H2 zinc finger domain. Tu was identified in all 38 Wty lines examined, and was completely absent from all 56 non-warty (nWty) lines. Cucumber plants transgenic for Tu (TCP) revealed that Tu was required for the Wty fruit phenotype. Subcellular localization showed that the fusion protein GFP-Tu was localized mainly to the nucleus. Based on analyses of semi-quantitative and quantitative reverse transcription polymerase chain reaction (RT-PCR), and mRNA in situ hybridization, we found that Tu was expressed specifically in fruit spine cells during development of fruit tubercules. Moreover, cytokinin (CTK) content measurements and cytological observations in Wty and nWty fruits revealed that the Wty fruit phenotype correlated with high endogenous CTK concentrations. As a result of further analyses on the transcriptomic profile of the nWty fruit epidermis and TCP fruit warts, expression of CTK-associated genes, and hormone content in nWty fruit epidermis, Wty fruit warts and epidermis, and TCP fruit warts and epidermis, we found that Tu probably promoted CTK biosynthesis in fruit warts. Here we show that Tu could not be expressed in the glabrous and tubercule-free mutant line gl that contained Tu, this result that futher confirmed the epistatic effect of the trichome (spine) gene Gl over Tu. Taken together, these data led us to propose a genetic pathway for the Wty fruit trait that could guide future mechanistic studies.

  3. Elongation factor Tu D138N, a mutant with modified substrate specificity, as a tool to study energy consumption in protein biosynthesis.

    Science.gov (United States)

    Weijland, A; Parlato, G; Parmeggiani, A

    1994-09-06

    Substitution Asp138-->Asn changes the substrate specificity of elongation factor (EF) Tu from GTP to XTP [Hwang & Miller (1987) J. Biol. Chem. 262, 13081-13085]. This mutated EF-Tu (EF-Tu D138N) was used to show that 2 XTP molecules are hydrolyzed for each elongation cycle [Weijland & Parmeggiani (1993) Science 259, 1311-1313]. Here we extend the study of the properties of this EF-Tu mutant and its function in the elongation process. In poly(U)-directed poly(phenylalanine) synthesis, the number of peptide chains synthesized using EF-Tu D138N.XTP was 30% higher than with EF-Tu wild type (wt).GTP. However, since in the former case the average peptide chain length was correspondingly reduced, the number of the residues incorporated turned out to be nearly the same in both systems. The K'd values of the XTP and XDP complexes of EF-Tu D138N were similar to those of the GTP and GDP complexes of EF-Tu wt. The extent of leucine misincorporation and the kirromycin effect were also comparable to those in the EF-Tu wt/GTP system. The hydrolysis of two XTP molecules, very likely as part of two EF-Tu D138N.XTP complexes, for each elongation cycle was found to be independent of (i) MgCl2 concentration, (ii) ribosome concentration, and (iii) temperature (5-40 degrees C). With rate-limiting amounts of XTP the K'm of its XTPase activity corresponded to the K'm for XTP of poly(phenylalanine) synthesis (0.3-0.6 microM).(ABSTRACT TRUNCATED AT 250 WORDS)

  4. The "Da Ming Hunyi Tu": Repurposing a Ming Map in Sino-African Diplomacy

    Directory of Open Access Journals (Sweden)

    Alexander Akin

    2016-12-01

    Full Text Available In 2002, an exhibition at South Africa’s parliament included a reproduction of the Da Ming Hunyi Tu (Amalgamated map of the Great Ming, citing it as the earliest world map to depict the entire African continent. As part of its broader efforts to shape a narrative of long-standing and peaceful international relations with Africa, the People’s Republic of China formally presented a replica of this map as a gift to the South African government in conjunction with the exhibition. In official statements and popular media coverage alike, the map was described as evidence of a distinctly Chinese approach to global relations, based on benevolence and mutual respect. In particular, the map was ahistorically intertwined with the legacy of Zheng He’s diplomatic expeditions, which reached the East African coast in the early 1400s. To the cartographic historian, however, the depiction of Africa in the Da Ming Hunyi Tu is clearly derived from non-Chinese sources that predate Zheng He’s expeditions. This article examines the ways in which the map has been divorced from its original context to suit modern needs, exemplifying the deployment of cartography to deflect anxieties about the nature of Chinese economic influence in South Africa.

  5. H1 - antihistamīna preparātu aprite aptiekās

    OpenAIRE

    Palamarčuka, Nadežda

    2009-01-01

    Maģistra darba mērķis bija noskaidrot, kādi H1-antihistamīna preparāti ir vislabāk pazīstami un tiek visvairāk pirkti aptiekās. Pētījumā tika aptaujāti 100 pacienti un analizēti lieltirgotavas MagnumMedical H1-antihistamīna preparātu tirdzniecības apjomi. Iegūtie rezultāti liecina, ka vislielākais H1-antihistamīna preparātu patēriņš ir pirmās paaudzes preparātiem Tavegyl (klemastīns) un Suprastin (hloropiramīns), jo to iegādei nav nepieciešamas receptes, tie ir salīdzinoši lēti un ir bijuši p...

  6. An Interbacterial NAD(P)+ Glycohydrolase Toxin Requires Elongation Factor Tu for Delivery to Target Cells

    Energy Technology Data Exchange (ETDEWEB)

    Whitney, John C.; Quentin, Dennis; Sawai, Shin; LeRoux, Michele; Harding, Brittany N.; Ledvina, Hannah E.; Tran, Bao Q.; Robinson, Howard; Goo, Young Ah; Goodlett, David R.; Raunser, Stefan; Mougous, Joseph D.

    2015-10-08

    Type VI secretion (T6S) influences the composition of microbial communities by catalyzing the delivery of toxins between adjacent bacterial cells. Here, we demonstrate that a T6S integral membrane toxin from Pseudomonas aeruginosa, Tse6, acts on target cells by degrading the universally essential dinucleotides NAD+ and NADP+. Structural analyses of Tse6 show that it resembles mono-ADP-ribosyltransferase proteins, such as diphtheria toxin, with the exception of a unique loop that both excludes proteinaceous ADP-ribose acceptors and contributes to hydrolysis. We find that entry of Tse6 into target cells requires its binding to an essential housekeeping protein, translation elongation factor Tu (EF-Tu). These proteins participate in a larger assembly that additionally directs toxin export and provides chaperone activity. Visualization of this complex by electron microscopy defines the architecture of a toxin-loaded T6S apparatus and provides mechanistic insight into intercellular membrane protein delivery between bacteria.

  7. TuBaFrost 5: multifunctional central database application for a European tumor bank.

    Science.gov (United States)

    Isabelle, M; Teodorovic, I; Morente, M M; Jaminé, D; Passioukov, A; Lejeune, S; Therasse, P; Dinjens, W N M; Oosterhuis, J W; Lam, K H; Oomen, M H A; Spatz, A; Ratcliffe, C; Knox, K; Mager, R; Kerr, D; Pezzella, F; van de Vijver, M; van Boven, H; Alonso, S; Kerjaschki, D; Pammer, J; Lopez-Guerrero, J A; Llombart Bosch, A; Carbone, A; Gloghini, A; van Veen, E-B; van Damme, B; Riegman, P H J

    2006-12-01

    Developing a tissue bank database has become more than just logically arranging data in tables combined with a search engine. Current demand for high quality samples and data, and the ever-changing legal and ethical regulations mean that the application must reflect TuBaFrost rules and protocols for the collection, exchange and use of tissue. To ensure continuation and extension of the TuBaFrost European tissue bank, the custodianship of the samples, and hence the decision over whether to issue samples to requestors, remains with the local collecting centre. The database application described in this article has been developed to facilitate this open structure virtual tissue bank model serving a large group. It encompasses many key tasks, without the requirement for personnel, hence minimising operational costs. The Internet-accessible database application enables search, selection and request submission for requestors, whereas collectors can upload and edit their collection. Communication between requestor and involved collectors is started with automatically generated e-mails.

  8. Desafio ao uso das preparações alcoólicas para higienização das mãos nos serviços de saúde

    Directory of Open Access Journals (Sweden)

    Maria Fernanda do Prado

    2014-09-01

    Full Text Available Objetivo: Propor uma reflexão teórica sobre os aspectos relacionados ao uso das preparações alcoólicas para higienização das mãos, no contexto dos serviços de saúde, na perspectiva das recomendações internacionais e nacionais, da eficácia antimicrobiana e fatores associados. Método: Reflexão teórica acerca do uso das preparações alcoólicas para a higienização das mãos nos serviços de saúde, fundamentada nas normativas internacionais e nacionais vigentes. Resultados: A comprovação da eficácia antimicrobiana das preparações alcoólicas por métodos rigorosos que simulam condições práticas de uso é fundamental para a utilização destes produtos nos serviços de saúde. Coexistem ainda outras variáveis envolvidas na eficácia do procedimento de higienização das mãos, tais como a sua duração, o volume do produto a ser aplicado e a aceitabilidade. Conclusão: Identificam-se lacunas nas normativas oficiais, referentes aos aspectos supracitados que podem comprometer um dos componentes mais importantes do controle de infecções e a segurança do paciente.

  9. Preparação e caracterização de nanocompósitos de nanotubos de carbono revestidos com nanopartículas de óxido de zinco

    OpenAIRE

    Rezende, Natália Pereira

    2015-01-01

    Neste trabalho foram preparados e caracterizados nanocompósitos de nanotubos de carbono (CNT) revestidos com nanopartículas de óxido de zinco (ZnO/CNT). Diversos estudos realizados mostram que filmes de ZnO/CNT podem ser aplicados em geradores piezoelétricos flexíveis, supercapacitores, biosensores de peróxido de hidrogênio, dentre outros. No presente trabalho, estudou-se a potencialidade de empregar esses filmes em supercapacitores. A preparação dos nanocompósitos foi dividida em etapas. Ini...

  10. Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation.

    Science.gov (United States)

    Choudhury, Nila Roy; Nowak, Jakub S; Zuo, Juan; Rappsilber, Juri; Spoel, Steven H; Michlewski, Gracjan

    2014-11-20

    RNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated pre-let-7 uridylation, inhibiting its processing and targeting it for degradation. Surprisingly, despite binding to other pre-microRNAs (pre-miRNAs), only pre-let-7 is efficiently uridylated by TuT4. Thus, we hypothesized the existence of substrate-specific cofactors that stimulate Lin28a-mediated pre-let-7 uridylation or restrict its functionality on non-let-7 pre-miRNAs. Through RNA pull-downs coupled with quantitative mass spectrometry, we identified the E3 ligase Trim25 as an RNA-specific cofactor for Lin28a/TuT4-mediated uridylation. We show that Trim25 binds to the conserved terminal loop (CTL) of pre-let-7 and activates TuT4, allowing for more efficient Lin28a-mediated uridylation. These findings reveal that protein-modifying enzymes, only recently shown to bind RNA, can guide the function of canonical ribonucleoprotein (RNP) complexes in cis, thereby providing an additional level of specificity.

  11. Trim25 Is an RNA-Specific Activator of Lin28a/TuT4-Mediated Uridylation

    Directory of Open Access Journals (Sweden)

    Nila Roy Choudhury

    2014-11-01

    Full Text Available RNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated pre-let-7 uridylation, inhibiting its processing and targeting it for degradation. Surprisingly, despite binding to other pre-microRNAs (pre-miRNAs, only pre-let-7 is efficiently uridylated by TuT4. Thus, we hypothesized the existence of substrate-specific cofactors that stimulate Lin28a-mediated pre-let-7 uridylation or restrict its functionality on non-let-7 pre-miRNAs. Through RNA pull-downs coupled with quantitative mass spectrometry, we identified the E3 ligase Trim25 as an RNA-specific cofactor for Lin28a/TuT4-mediated uridylation. We show that Trim25 binds to the conserved terminal loop (CTL of pre-let-7 and activates TuT4, allowing for more efficient Lin28a-mediated uridylation. These findings reveal that protein-modifying enzymes, only recently shown to bind RNA, can guide the function of canonical ribonucleoprotein (RNP complexes in cis, thereby providing an additional level of specificity.

  12. The YvfTU Two-component System is involved in plcR expression in Bacillus cereus

    NARCIS (Netherlands)

    Brillard, Julien; Susanna, Kim; Michaud, Caroline; Dargaignaratz, Claire; Gohar, Michel; Nielsen-Leroux, Christina; Ramarao, Nalini; Kolsto, Anne-Brit; Nguyen-The, Christophe; Lereclus, Didier; Broussolle, Veronique

    2008-01-01

    Background: Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative

  13. 2011 Lasker Clinical Medical Research Award Honors Prof.TU You-you for the Discovery of Artemisinin

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    The 2011 Lasker Clinical Medical Research Award honors a scientist who discovered artemisinin and its utility for treating malaria.Prof.TU You-you (China Academy of Chinese Medical Sciences,Beijing) transformed an ancient Chinese healing method into the most powerful antimalarial medicine currently available that has saved millions of lives across the globe,especially in the developing world.

  14. A guidelines handbook for GPR surveys in tunnels: a COST Action TU1208 contribution

    Science.gov (United States)

    Bianchini Ciampoli, Luca; Alani, Amir M.; Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Tosti, Fabio

    2016-04-01

    A significant open issue concerning the reliability of geophysical methods and in particular of ground penetrating radar (GPR), both in research and professional context, is a general lack of international standards. This is a major problem to be faced, in order to gain scientific strictness for the GPR practices, and to easily extend to the international community the results achieved within the area of single virtuous countries. Producing international guidelines can represent an important step forward, in this sense. In the memorandum of understanding of the COST Action TU1208 is clearly stated that one of the main purposes of the Action is the "development of innovative protocols and guidelines which will be published in a handbook and constitute a basis for European Standards, for an effective GPR application in CE tasks; safety, economic and financial criteria will be integrated within the protocols". Of course this is not a simple task to be accomplished. Firstly, survey procedures are highly dependent on the objective of the survey itself. On the basis of the objective of each geophysical test, the GPR system, the antenna configuration, and even the processing procedures may change. Besides, these procedures are also influenced by the environmental conditions in which the tests are performed. This affects several aspects spanning from hardware to software, but including, for instance, also safety issues. Due to these reasons, one of the main goal of the COST Action TU1208 is the development of several guidelines related to the main applications of GPR in the field of civil engineering. In this work, the structure of a guidelines handbook for GPR activities in tunnels is outlined. In the first sections, the principal references in the field are provided, and the most common GPR equipment and complementary technologies are described. Subsequently, the survey methodologies are explained. Particular attention is paid to the preliminary activities to be carried

  15. Preventive Effect of TU-100 on a Type-2 Model of Colitis in Mice: Possible Involvement of Enhancing Adrenomedullin in Intestinal Epithelial Cells

    Directory of Open Access Journals (Sweden)

    Atsushi Kaneko

    2013-01-01

    Full Text Available Purpose. Crohn's disease (CD and ulcerative colitis (UC, the two major forms of inflammatory bowel disease (IBD, have histopathologically and immunologically different characteristics. We previously reported that a traditional Japanese medicine, daikenchuto (TU-100, ameliorated a trinitrobenzenesulfonic acid- (TNBS- induced type-1 model colitis exhibiting histopathological features of CD through adrenomedullin (ADM enhancement. Our current aims were to examine whether TU-100 ameliorates a type-2 model colitis that histologically resembles UC and identify the active ingredients. Methods. TU-100 was administered orally to mice with oxazolone- (OXN- induced type-2 model colitis. The morbidity was evaluated by body weight loss and the macroscopic score of colonic lesions. ADM was quantified using an EIA kit. Results. TU-100 prevented weight loss and colon ulceration. ADM production by intestinal epithelial cells was increased by TU-100 addition. Screening to identify active ingredients showed that [6]-shogaol and hydroxy α-sanshool enhanced ADM production. Conclusions. TU-100 exerted a protective effect in OXN-induced type-2 model colitis, indicating that TU-100 may be a beneficial agent for treatment of UC.

  16. Comparação da fase preparação do triplo hop test em mulheres com e sem síndrome da dor femoropatelar: análise cinemática, cinética e eletromiográfica

    OpenAIRE

    Bley, André Serra

    2013-01-01

    A síndrome da dor femoropatelar (SDFP) é uma condição musculoesquelética ligada ao mau alinhamento dinâmico dos membros inferiores. Altas demandas musculares são aplicadas sobre o membro inferior durante a fase de preparação do triplo hop test (THT) que podem influenciar o comportamento biomecânico do membro inferior. O objetivo deste estudo transversal caso-controle foi comparar à cinemática, cinética e atividade muscular do tronco e membro inferior durante a fase de preparação do THT em mul...

  17. Preparação de sóis aquosos de pseudoboemita com fibrilas de comprimentos diferentes Preparation of pseudoboehmite aqueous sols with fibril of different lengths

    Directory of Open Access Journals (Sweden)

    P. S. Santos

    2009-06-01

    Full Text Available O mineral boemita, de composição (AlOOH, na forma sintética pode ter estruturas cristalinas denominadas "boemita bem-cristalizada", "pseudoboemita" e "pseudoboemita fibrilar", caracterizáveis por difração de raios X e por microscopia eletrônica de transmissão. É feita uma revisão das propriedades e das aplicações desses tipos de boemita em cerâmica, catalisadores e ab/adsorventes, enfatizando a especificidade do tipo nas diversas aplicações. É descrito um método de preparação de sóis e géis aquosos de pseudoboemita fibrilar, muito pura, usando matérias-primas nacionais: alumínio em pó e ácido acético, não precisando usar Hg2+ como "ativador" da reação. Controlando o tempo de envelhecimento térmico dos produtos da reação, obtêm-se fibrilas de comprimento médio desde 53 nm até 720 nm. O método permite também preparar sóis aquosos monofásicos e bifásicos, bem como cristais coloidais de pseudoboemita fibrilar. As fibrilas de pseudoboemita com até 168 h de envelhecimento térmico são "high surface materials" porque sua área de superfície específica é maior que 100 m²/g.The mineral boehmite, of composition (AlOOH, as a synthetic material may have crystalline structures named "well-crystallized boehmite", "pseudobehmite", and "fibrillar pseusoboehmite", characterized by X-ray diffraction and transmission electron microscopy. A review is made of the properties and applications of these boehmite types in ceramics, catalysts and ab/adsorvents, calling attention to the specificity of the structure type in the several applications. A method is described to prepare aqueous sols and gels of fibrillar pseudoboehmite, very pure, using Brazilian raw materials: aluminum powder and acetic acid, avoiding Hg2+ as "activator" of the reaction. Controlling the thermal ageing time of the reaction products, fibrils are obtained with average length from 53 nm to 720 nm. The method also allows the preparation of monolayer and

  18. Avaliação do teor de antioxidantes, flavonoides e compostos fenólicos em preparações condimentares

    Directory of Open Access Journals (Sweden)

    G.M. MENDES

    2015-06-01

    Full Text Available RESUMO:Plantas condimentares são amplamente utilizadas na culinária como alimento funcional. Pacientes hipertensos, que retiram o sal de cozinha (NaCl de sua alimentação, relatam que os alimentos "perdem" o sabor. Visando alternativas para substituir o sal e melhorar a palatabilidade das preparações alimentares de pacientes hipertensos, o objetivo deste estudo foi desenvolver temperos à base de plantas medicinais e analisar os teores de compostos fenólicos, flavonoides e atividade antioxidante destes. Plantas (coentro, salsa, orégano, alecrim, manjericão, alho, cebola cultivadas organicamente em Viçosa (MG foram processadas e os temperos foram desenvolvidos a partir de combinação destas espécies, de acordo com teste aceitabilidade sensorial afetivo por meio de escala hedônica. Foram definidos três temperos, dos quais foram feitos extratos metanólicos por remaceração até esgotamento. Nas dosagens utilizou-se DPPH na avaliação da atividade antioxidante; reagente de Folin-Ciocalteu para compostos fenólicos e vanilina clorídrica para flavonoides. As análises foram feitas com quatro repetições e os resultados submetidos à análise de variância e teste de média a 5% de significância. Na aceitação subjetiva dos temperos a nota média foi 7,07±1,09 e 8,0±0,93 na ação afetiva como alimento. O teor mais elevado de compostos fenólicos foi no tempero 2 (alho, salsa e manjericão (55,04±4,307 mg/mL, a maior ação antioxidante foi no tempero 1 (alho, cebola, coentro e orégano nos tempos 0 (48,72% e 30 minutos (78,37%. Os teores de flavonoides foram semelhantes nos 3 temperos. Todos os temperos tiveram boa aceitação por pacientes hipertensos e mostraram-se promissores na substituição dos condimentos convencionais.

  19. Mutation of the conserved Gly94 and Gly126 in elongation factor Tu from Escherichia coli. Elucidation of their structural and functional roles

    DEFF Research Database (Denmark)

    Knudsen, Charlotte Rohde; Kjaersgård, I V; Wiborg, O

    1995-01-01

    All guanine-nucleotide-binding proteins cycle between an inactive, GDP-bound and an active, GTP-bound conformation whereby they function as molecular switches. Elongation factor Tu from Escherichia coli is used as a model for defining residues important in the switch mechanism. Gly94 and Gly126...... for GDP over GTP. Furthermore the [G94A]EF-Tu mutant possesses an increased GTPase activity. The aminoacyl-tRNA affinity is much reduced for [G94A]EF-Tu, as reflected in an increase of the dissociation rate constant for the ternary complex by a factor of 40. Surprisingly, however, both mutants...... in their GDP forms have a low, but significant affinity for aminoacyl-tRNA, which is not seen for the wild-type elongation factor Tu. The mutants only exhibit minor changes compared to the wild type with respect to in vitro translation of a poly(U) messenger. Udgivelsesdato: 1995-Feb-15...

  20. Collaboration of the Dutch research program for radioactive waste disposal (OPERA) and TU Delft

    Energy Technology Data Exchange (ETDEWEB)

    Bykov, D.M.; Kloosterman, J.L. [TU Delft (Netherlands). Reactor Inst. Delft; Neeft, E.A.C.; Verhoef, E.V. [COVRA N.V., Nieuwdorp (Netherlands)

    2015-07-01

    awarded research proposals available at www.covra.nl/downloads/o pera. Building up competences and knowledge on radioactive waste management and geological disposal is an important goal of OPERA. To this end OPERA collaborates with TU Delft to develop an academic curriculum for the chair Chemistry of the Nuclear Fuel Cycle in the Master of Science Engineering. Furthermore, the results obtained in OPERA will be presented at a Summer School which is planned to be organized at the end of the Program. TU Delft educates young specialists, bachelor and master students, by research projects on geological disposal at the new actinide laboratory (U and Th). For such purpose the knowledge transfer from other OPERA partners is also foreseen, including students internships and visits of the research facilities. Reactor Institute Delft (RID) is part of the Applied Sciences faculty of TU Delft and houses the Radiation Science and Technology (RST) department. RID operates the Hoger Onderwijs Reactor (HOR), a 2 MW pool- type research reactor in an academic setting. The reactor is used as a source of neutrons and positrons for research purposes, including those of OPERA. It also provides neutrons to a variety of facilities for radioisotope production and neutron activation analysis. OPERA is financed by the Dutch Ministry of Economic Affairs and the public limited liability company Elektriciteits-Produktiemaatschappij Zuid-Nederland (EPZ) and coordinated by COVRA.

  1. Development of SAP-DoA techniques for GPR data processing within COST Action TU1208

    Science.gov (United States)

    Meschino, Simone; Pajewski, Lara; Marciniak, Marian

    2016-04-01

    This work focuses on the use of Sub-Array Processing (SAP) and Direction of Arrival (DoA) approaches for the processing of Ground-Penetrating Radar data, with the purpose of locating metal scatterers embedded in concrete or buried in the ground. Research activities have been carried out during two Short-Term Scientific Missions (STSMs) funded by the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in May 2015 and January 2016. In applications involving smart antennas and in the presence of several transmitters operating simultaneously, it is important for a receiving array to be able to estimate the Direction of Arrival (DoA) of the incoming signals, in order to decipher how many emitters are present and predict their positions. A number of methods have been devised for DoA estimation: the MUltiple SIgnal Classification (MUSIC) and Estimation of Signal Parameters via Rotational Invariance Technique (ESPRIT) are amongst the most popular ones [1]. In the scenario considered by us, the electromagnetic sources are the currents induced on metal elements embedded in concrete or buried in the ground. GPR radargrams are processed, to estimate the DoAs of the electric field back-scattered by the sought targets. In order to work in near-field conditions, a sub-array processing (SAP) approach is adopted: the radargram is partitioned in sub-radargrams composed of few A-scans each, the dominant DoA is predicted for each sub-radargram. The estimated angles are triangulated, obtaining a set of crossings with intersections condensed around object locations. This pattern is filtered, in order to remove a noisy background of unwanted crossings, and is processed by applying the statistical procedure described in [2]. We tested our approach on synthetic GPR radargrams, obtained by using the freeware simulator gprMax implementing the Finite-Difference Time-Domain method [3]. In particular, we worked with

  2. Critical control points for meat balls and kibbe preparations in a hospital kitchen Pontos críticos de controle na preparação de almôndegas e kibes em uma cozinha hospitalar

    Directory of Open Access Journals (Sweden)

    Débora Midori Myaki Pedroso

    1999-12-01

    Full Text Available Hazards and critical control points (CCP associated with meat balls and kibbe preparations in a hospital kitchen were determined using flow diagrams and microbiological testing of samples collected along the production line. Microbiological testing included counts of mesophilic and psicrothrophic microorganisms, yeasts and molds, total and fecal coliforms, C. perfringens, coagulase positive staphylococci, bacteria of the B. cereus group and detection of Salmonella. Time/temperature binomial was measured in all steps of preparation. A decision tree was used to help in the determination of CCPs. The detected hazards were: contamination of raw meat and vegetables, multiplication of the microorganisms during meat manipulation, poor hygiene of utensils and equipment, and survival of microorganisms to the cooking process. Cooking and hot-holding were considered CCPs. The results stress the importance of the implementation of a training program for nutricionists and foodhandlers and the monitoring of CCPs and other measures to prevent foodborne diseases.Foram determinados os perigos e os pontos críticos de controle (PCC associados a preparações de almôndegas e kibes em uma cozinha hospitalar, baseados em fluxogramas de preparo e análises microbiológicas de amostras coletadas ao longo da preparação. As análises microbiológicas incluíram a contagem de microrganismos mesófilos, psicrotróficos, bolores e leveduras, C. perfringens, estafilococos coagulase positiva, bactérias do grupo B. cereus, número mais provável de coliformes totais e fecais e pesquisa de Salmonella. Foram medidos o tempo e a temperatura em todas as etapas da preparação. Para a determinação dos PCCs foi utilizada uma árvore decisória. Foram detectados os seguintes perigos: a contaminação da carne e vegetais crus, a multiplicação dos microrganismos durante a etapa de manipulação da carne, a falta de higiene dos utensílios equipamentos, a sobrevivência de

  3. Sobre a preparação e a composição em terapia de grupo: descrições construcionistas sociais Concerning preparation and composition in group therapy: social constructionist descriptions

    Directory of Open Access Journals (Sweden)

    Emerson F. Rasera

    2006-01-01

    Full Text Available A definição construcionista do grupo terapêutico como uma prática discursiva enfatiza o caráter de negociação do processo de preparação e composição do grupo. Considerando esta perspectiva, o objetivo deste trabalho é compreender como determinadas formas de conversar contribuem para o processo de preparação e composição de um grupo de apoio para portadores do HIV. O grupo estudado consistiu de 10 sessões com 4 participantes. As sessões foram gravadas e transcritas. Através da análise discursiva dos momentos grupais, foi possível descrever a preparação para o grupo através de três processos conversacionais de: co-responsabilização do participante e construção inicial do pedido; co-responsabilização do terapeuta e renegociação do contrato; e 'aproximação discursiva' das descrições dos participantes. Em relação à composição do grupo, a inclusão no grupo não foi ditada a priori a partir de características do indivíduo, mas implicou um exercício de 'engajamento relacional' entre terapeuta e participantes.The constructionist definition of group therapy as a discursive practice emphasizes the character of negotiation in the process of group preparation and composition. Considering this perspective, the objective of this study is to comprehend how certain ways of talking contribute to the process of preparing and composing a support group for people living with HIV. The group that was studied had 10 sessions with 4 participants. The sessions were tape recorded and transcribed. By using discursive analysis on the transcripts of the group sessions, it was possible to describe the preparation for the group as involving three conversational processes: initial construction of demand and the participant's co-responsibility; renegotiation of the contract and the therapist's co-responsibility; and the 'discursive approach' of participant's descriptions. In relation to group composition, the inclusion of the

  4. Preparação e caracterização inicial de complexo de inclusão entre nitrofurazona e 2-hidroxipropil-β-ciclodextrina

    Directory of Open Access Journals (Sweden)

    L. F. Fraceto

    2009-01-01

    Full Text Available

    Nitrofurazona (NF, 5-nitro-2-furaldeído semicarbazona, é um antibiótico de amplo espectro, que apresenta diversos efeitos tóxicos e baixa solubilidade aquosa. A complexação da NF com ciclodextrinas é de grande interesse para o desenvolvimento de uma formulação para este antibiótico que seja mais segura e eficiente. Neste trabalho foi realizada a preparação e caracterização inicial do complexo de inclusão entre NF e hidroxipropil-ß-ciclodextrina (HP-ß-CD através de experimentos para determinação da cinética de complexação, medidas de fotoestabilidade, medidas de constante de afinidade fármaco: ciclodextrina, ensaios de liberação in vitro, estequiometria de formação do complexo e morfologia do complexo por microscopia eletrônica de varredura. Os ensaios de cinética de complexação mostram que para o complexo atingir o equilíbrio são necessárias 17,3h. As isotermas de solubilidade determinadas para a NF em função da temperatura mostraram perfis do tipo A e B indicando que a temperatura é um fator importante na complexação da NF com ciclodextrina. Os experimentos de fotoestabilidade indicam que a inserção da molécula de NF na cavidade interna da ciclodextrina protege o fármaco da fotodecomposição. A cinética de liberação mostra que o perfil de liberação do fármaco é modificado pela presença da ciclodextrina no meio. A estequiometria de complexação entre NF e HP-ß-CD determinada foi de 1:1 NF:HP-ß-CD. Os resultados de microscopia eletrônica de varredura indicam alterações na estrutura cristalina da NF em presença de ciclodextrina. Este estudo está baseado na caracterização físico-química da complexação entre NF e HP-ß-CD podendo ser uma nova potencial opção para utilização terapêutica do NF. Palavras-chave: nitrofurazona; ciclodextrina; complexo de inclus

  5. La ciudad-mito y lo grotesco en los hermanos González Tuñón

    OpenAIRE

    2015-01-01

    La poética de los hermanos González Tuñón, iniciada al calor de las vanguardias porteñas, es estudiada atendiendo a la mítica de lo urbano, lo que permite a su vez analizar el empleo de la estética grotesca como modo de subrayar una cultura híbrid

  6. BnSGS3 Has Differential Effects on the Accumulation of CMV, ORMV and TuMV in Oilseed Rape

    Directory of Open Access Journals (Sweden)

    Quan Chen

    2015-07-01

    Full Text Available Virus diseases greatly affect oilseed rape (Brassica napus production. Investigating antiviral genes may lead to the development of disease-resistant varieties of oilseed rape. In this study, we examined the effects of the suppressor of gene silencing 3 in Brassica napus (BnSGS3, a putative antiviral gene with different genus viruses by constructing BnSGS3-overexpressing (BnSGS3-Ov and BnSGS3-silenced (BnSGS3-Si oilseed rape (cv. Zhongshuang No. 6 plants. These three viruses are Oilseed rape mosaic virus (ORMV, Turnip mosaic virus (TuMV and Cucumber mosaic virus (CMV. The native BnSGS3 expressed in all examined tissues with the highest expression in siliques. All three viruses induced BnSGS3 expression, but ORMV induced a dramatic increase in the BnSGS3-Ov plants, followed by TuMV and CMV. Upon inoculation with three different viruses, transcript abundance of BnSGS3 gene follows: BnSGS3-Ov > non-transgenic plants > BnSGS3-Si. The accumulation quantities of ORMV and TuMV exhibited a similar trend. However, CMV accumulation showed an opposite trend where virus accumulations were negatively correlated with BnSGS3 expression. The results suggest that BnSGS3 selectively inhibits CMV accumulation but promotes ORMV and TuMV accumulation. BnSGS3 should be used in different ways (up- and down-regulation for breeding virus-resistant oilseed rape varieties.

  7. EFFECTS OF DIFFERENT CONCENTRATIONS OF ANTISENSE OLIGODEOXYNUCLEOTIDES(ASODN) ON HUMAN PANCREATIC CANCER CELL LINE PaTu8988s

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Objective: To study the effects of different concentrations of antisense oligodeoxynucleotides (ASODN) on human pancreatic cancer cell line PaTu8988s. Method: Human pancreatic cancer cell line PaTu8988s in exponential growth stage was used to study the effect of different drug concentrations on the cell line in the presence of different concentrations (0 μg/ml, 5 μg/ml, 25 μg/ml, 50 μg/ml, 10 μg/m, 200 μg/ml) of ASODN and sense oligodeoxynucleotides (SODN). Cell counts and 3-[4,5-dimethylthiazolzyl]-2,5-diphenyl tetrazolium bromide (MTT) assays were carried out. Results: The inhibitory rate on the cell line PaTu8988s was 98.73%,95.76%,69.49%,33.05% and 0 for ASODM concentrations of 200 μg/ml,100 μg/ml,50 μg/ml,25 μg/ml and 5 μg/ml at 48 hours. Conclusions: K-ras complementary ASODN can inhibit the growth of human pancreatic cancer cell line PaTu8988s by 30.05% to 98.73%. This is likely to contribute to the specificity of the K-ras mRNA complementary capped ASODN sequential codon. Non-specific effect and side effect of ASOND were observed for instance, the greater the concentration is, the earlier the peak of inhibitory rate is reached. In concentration of 25 μg/ml to 100 μg/ml ASODN showed a dose-effect correlation

  8. A conserved P-loop anchor limits the structural dynamics that mediate nucleotide dissociation in EF-Tu

    Science.gov (United States)

    Mercier, Evan; Girodat, Dylan; Wieden, Hans-Joachim

    2015-01-01

    The phosphate-binding loop (P-loop) is a conserved sequence motif found in mononucleotide-binding proteins. Little is known about the structural dynamics of this region and its contribution to the observed nucleotide binding properties. Understanding the underlying design principles is of great interest for biomolecular engineering applications. We have used rapid-kinetics measurements in vitro and molecular dynamics (MD) simulations in silico to investigate the relationship between GTP-binding properties and P-loop structural dynamics in the universally conserved Elongation Factor (EF) Tu. Analysis of wild type EF-Tu and variants with substitutions at positions in or adjacent to the P-loop revealed a correlation between P-loop flexibility and the entropy of activation for GTP dissociation. The same variants demonstrate more backbone flexibility in two N-terminal amino acids of the P-loop during force-induced EF-Tu·GTP dissociation in Steered Molecular Dynamics simulations. Amino acids Gly18 and His19 are involved in stabilizing the P-loop backbone via interactions with the adjacent helix C. We propose that these P-loop/helix C interactions function as a conserved P-loop anchoring module and identify the presence of P-loop anchors within several GTPases and ATPases suggesting their evolutionary conservation. PMID:25566871

  9. A conserved P-loop anchor limits the structural dynamics that mediate nucleotide dissociation in EF-Tu

    Science.gov (United States)

    Mercier, Evan; Girodat, Dylan; Wieden, Hans-Joachim

    2015-01-01

    The phosphate-binding loop (P-loop) is a conserved sequence motif found in mononucleotide-binding proteins. Little is known about the structural dynamics of this region and its contribution to the observed nucleotide binding properties. Understanding the underlying design principles is of great interest for biomolecular engineering applications. We have used rapid-kinetics measurements in vitro and molecular dynamics (MD) simulations in silico to investigate the relationship between GTP-binding properties and P-loop structural dynamics in the universally conserved Elongation Factor (EF) Tu. Analysis of wild type EF-Tu and variants with substitutions at positions in or adjacent to the P-loop revealed a correlation between P-loop flexibility and the entropy of activation for GTP dissociation. The same variants demonstrate more backbone flexibility in two N-terminal amino acids of the P-loop during force-induced EF-Tu.GTP dissociation in Steered Molecular Dynamics simulations. Amino acids Gly18 and His19 are involved in stabilizing the P-loop backbone via interactions with the adjacent helix C. We propose that these P-loop/helix C interactions function as a conserved P-loop anchoring module and identify the presence of P-loop anchors within several GTPases and ATPases suggesting their evolutionary conservation.

  10. Knitting Mochilas: A Sociocultural, Developmental Practice in Arhuaco Indigenous Communities

    Directory of Open Access Journals (Sweden)

    Lilian Patricia Rodríguez-Burgos

    2016-05-01

    Full Text Available The purpose of this article is to analyze the psycho-cultural processes involved in knitting “mochilas” (traditional bags, a common craft in the Arhuaco indigenous community located in the Sierra Nevada de Santa Marta, Colombia. The article is structured in three parts, as follows: first, issues related to child development are discussed; then, the analysis method used to study the processes involved in the practice of knitting is presented and, finally, we reflect on the importance of recovering the sense and meaning of this everyday practice as a way to study child development.

  11. Knitting Mochilas: A Sociocultural, Developmental Practice in Arhuaco Indigenous Communities

    Science.gov (United States)

    Rodríguez-Burgos, Lilian Patricia; Rodríguez-Castro, Jennifer; Bojacá-Rodríguez, Sandra Milena; Izquierdo-Martínez, Dwrya Elena; Amórtegui-Lozano, Allain Alexander; Prieto-Castellanos, Miguel Angel

    2016-01-01

    The purpose of this article is to analyze the psycho-cultural processes involved in knitting “mochilas” (traditional bags), a common craft in the Arhuaco indigenous community located in the Sierra Nevada de Santa Marta, Colombia. The article is structured in three parts, as follows: first, issues related to child development are discussed; then, the analysis method used to study the processes involved in the practice of knitting is presented and, finally, we reflect on the importance of recovering the sense and meaning of this everyday practice as a way to study child development. PMID:27298634

  12. Preparação e caracterização de nanoemulsões contendo oligonucleotídeos antisense através da técnica de emulsificação espontânea

    OpenAIRE

    2002-01-01

    Recentemente, nanoemulsões preparadas pela técnica de microfluidização foram propostas como um sistema de liberação de oligonucleotídeos antisense (ON) por Teixeira et al. [J. contr. rel., 70:243-255, 2001]. O objetivo do presente trabalho foi preparar e caracterizar nanoemulsões através de uma técnica alternativa, a emulsificação espontânea, visando à preparação de pequenos lotes de produto final. As formulações foram caracterizadas em termos de diâmetro de partícula, potencial zeta e estabi...

  13. Electromagnetic simulators for Ground Penetrating Radar applications developed in COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Giannopoulos, Antonios; Warren, Craig; Antonijevic, Sinisa; Doric, Vicko; Poljak, Dragan

    2017-04-01

    Founded in 1971, COST (European COoperation in Science and Technology) is the first and widest European framework for the transnational coordination of research activities. It operates through Actions, science and technology networks with a duration of four years. The main objective of the COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (4 April 2013 - 3 October 2017) is to exchange and increase knowledge and experience on Ground-Penetrating Radar (GPR) techniques in civil engineering, whilst promoting in Europe a wider use of this technique. Research activities carried out in TU1208 include all aspects of the GPR technology and methodology: design, realization and testing of radar systems and antennas; development and testing of surveying procedures for the monitoring and inspection of structures; integration of GPR with other non-destructive testing approaches; advancement of electromagnetic-modelling, inversion and data-processing techniques for radargram analysis and interpretation. GPR radargrams often have no resemblance to the subsurface or structures over which the profiles were recorded. Various factors, including the innate design of the survey equipment and the complexity of electromagnetic propagation in composite scenarios, can disguise complex structures recorded on reflection profiles. Electromagnetic simulators can help to understand how target structures get translated into radargrams. They can show the limitations of GPR technique, highlight its capabilities, and support the user in understanding where and in what environment GPR can be effectively used. Furthermore, electromagnetic modelling can aid the choice of the most proper GPR equipment for a survey, facilitate the interpretation of complex datasets and be used for the design of new antennas. Electromagnetic simulators can be employed to produce synthetic radargrams with the purposes of testing new data-processing, imaging and inversion algorithms, or assess

  14. [Control of Aedes aegypti breeding sites with the program Recicla por tu bienestar in Merida, Mexico].

    Science.gov (United States)

    Barrera-Pérez, Mario A; Pavía-Ruz, Norma; Mendoza-Mezquita, Jorge Eduardo; Torres-Arcila, Nerio; Hernández-Hernández, Ramón; Castro-Gamboa, Francisco; Geded-Moreno, Eduardo; Cohuo-Rodríguez, Azael; Medina-Barreiro, Anuar; Koyoc-Cardeña, Edgar; Gómez-Dantés, Héctor; Kroeger, Axel; Vázquez-Prokopec, Gonzalo; Manrique-Saide, Pablo

    2015-01-01

    To determine the importance of Ae. aegypti breeding-sites in Merida;to evaluate the impact of Recicla por tu bienestar (RxB, a recycling program) on the reduction of breeding sites and the perception of participants. The relative importance for pupae production of the different types of breeding-sites was determined. Pre-and post-RxB entomological surveys were performed in participant neighborhoods to evaluate the impact on total containers and positive breeding-sites. A survey on the perception of participating people about dengue prevention and control and RxB was applied. Buckets/pots and "small diverse items" were the most important breeding-sites. RxB had a significant impact in the reduction of total containers (IRR = 0.74), positive containers (IRR = 0.33) and the risk of a house being positive for Ae. aegypti (OR = 0.41). All the interviewed participants referred RxB as needed and most consider it useful. RxB should be considered as a good practice for the dengue vector control.

  15. TU Comae Berenices : Blazhko RR Lyrae Star in a Potential Binary System

    CERN Document Server

    de Ponthiere, Pierre; Menzies, Kenneth; Sabo, Richard

    2016-01-01

    We present the results of a photometry campaign of TU Com performed over a five-year time span. The analysis showed that the possible Blazhko period of 75 days published by the General Catalogue of Variable Stars is not correct. We identified two Blazhko periods of 43.6 and 45.5 days. This finding is based on measurement of 124 light maxima. A spectral analysis of the complete light curve confirmed these two periods. Besides the Blazhko amplitude and phase modulations, another long term periodic phase variation has been identified. This long term periodic variation affects the times of maximum light only and can be attributed to a light-travel time effect due to orbital motion of a binary system. The orbital parameters have been estimated by a nonlinear least-square fit applied to the set of (O-C) values. The Levenberg-Marquart algorithm has been used to perform the nonlinear least-square fit. The tentative orbital parameters include an orbital period of 1676 days, a minimal semi-major axis of 1.55 AU and a s...

  16. TU-100 (Daikenchuto) and ginger ameliorate anti-CD3 antibody induced T cell-mediated murine enteritis: microbe-independent effects involving Akt and NF-κB suppression.

    Science.gov (United States)

    Ueno, Nobuhiro; Hasebe, Takumu; Kaneko, Atsushi; Yamamoto, Masahiro; Fujiya, Mikihiro; Kohgo, Yutaka; Kono, Toru; Wang, Chong-Zhi; Yuan, Chun-Su; Bissonnette, Marc; Chang, Eugene B; Musch, Mark W

    2014-01-01

    The Japanese traditional medicine daikenchuto (TU-100) has anti-inflammatory activities, but the mechanisms remain incompletely understood. TU-100 includes ginger, ginseng, and Japanese pepper, each component possessing bioactive properties. The effects of TU-100 and individual components were investigated in a model of intestinal T lymphocyte activation using anti-CD3 antibody. To determine contribution of intestinal bacteria, specific pathogen free (SPF) and germ free (GF) mice were used. TU-100 or its components were delivered by diet or by gavage. Anti-CD3 antibody increased jejunal accumulation of fluid, increased TNFα, and induced intestinal epithelial apoptosis in both SPF and GF mice, which was blocked by either TU-100 or ginger, but not by ginseng or Japanese pepper. TU-100 and ginger also blocked anti-CD3-stimulated Akt and NF-κB activation. A co-culture system of colonic Caco2BBE and Jurkat-1 cells was used to examine T-lymphocyte/epithelial cells interactions. Jurkat-1 cells were stimulated with anti-CD3 to produce TNFα that activates epithelial cell NF-κB. TU-100 and ginger blocked anti-CD3 antibody activation of Akt in Jurkat cells, decreasing their TNFα production. Additionally, TU-100 and ginger alone blocked direct TNFα stimulation of Caco2BBE cells and decreased activation of caspase-3 and polyADP ribose. The present studies demonstrate a new anti-inflammatory action of TU-100 that is microbe-independent and due to its ginger component.

  17. DNA barcoding for identification of 'Candidatus Phytoplasmas' using a fragment of the elongation factor Tu gene.

    Directory of Open Access Journals (Sweden)

    Olga Makarova

    Full Text Available BACKGROUND: Phytoplasmas are bacterial phytopathogens responsible for significant losses in agricultural production worldwide. Several molecular markers are available for identification of groups or strains of phytoplasmas. However, they often cannot be used for identification of phytoplasmas from different groups simultaneously or are too long for routine diagnostics. DNA barcoding recently emerged as a convenient tool for species identification. Here, the development of a universal DNA barcode based on the elongation factor Tu (tuf gene for phytoplasma identification is reported. METHODOLOGY/PRINCIPAL FINDINGS: We designed a new set of primers and amplified a 420-444 bp fragment of tuf from all 91 phytoplasmas strains tested (16S rRNA groups -I through -VII, -IX through -XII, -XV, and -XX. Comparison of NJ trees constructed from the tuf barcode and a 1.2 kbp fragment of the 16S ribosomal gene revealed that the tuf tree is highly congruent with the 16S rRNA tree and had higher inter- and intra- group sequence divergence. Mean K2P inter-/intra- group divergences of the tuf barcode did not overlap and had approximately one order of magnitude difference for most groups, suggesting the presence of a DNA barcoding gap. The use of the tuf barcode allowed separation of main ribosomal groups and most of their subgroups. Phytoplasma tuf barcodes were deposited in the NCBI GenBank and Q-bank databases. CONCLUSIONS/SIGNIFICANCE: This study demonstrates that DNA barcoding principles can be applied for identification of phytoplasmas. Our findings suggest that the tuf barcode performs as well or better than a 1.2 kbp fragment of the 16S rRNA gene and thus provides an easy procedure for phytoplasma identification. The obtained sequences were used to create a publicly available reference database that can be used by plant health services and researchers for online phytoplasma identification.

  18. Civil Engineering Applications of Ground Penetrating Radar: Research Perspectives in COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Slob, Evert; Tosti, Fabio

    2013-04-01

    can be used by GPR operators to identify the signatures generated by uncommon targets or by composite structures. Repeated evaluations of the electromagnetic field scattered by known targets can be performed by a forward solver, in order to estimate - through comparison with measured data - the physics and geometry of the region investigated by the GPR. It is possible to identify three main areas, in the GPR field, that have to be addressed in order to promote the use of this technology in the civil engineering. These are: a) increase of the system sensitivity to enable the usability in a wider range of conditions; b) research novel data processing algorithms/analysis tools for the interpretation of GPR results; c) contribute to the development of new standards and guidelines and to training of end users, that will also help to increase the awareness of operators. In this framework, the COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar", proposed by Lara Pajewski, "Roma Tre" University, Rome, Italy, has been approved in November 2012 and is going to start in April 2013. It is a 4-years ambitious project already involving 17 European Countries (AT, BE, CH, CZ, DE, EL, ES, FI, FR, HR, IT, NL, NO, PL, PT, TR, UK), as well as Australia and U.S.A. The project will be developed within the frame of a unique approach based on the integrated contribution of University researchers, software developers, geophysics experts, Non-Destructive Testing equipment designers and producers, end users from private companies and public agencies. The main objective of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst promoting the effective use of this safe and non-destructive technique in the monitoring of systems. In this interdisciplinary Action, advantages and limitations of GPR will be highlighted, leading to the identification of gaps in knowledge and technology

  19. COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar": ongoing research activities and third-year results

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Tosti, Fabio

    2016-04-01

    This work aims at disseminating the ongoing research activities and third-year results of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar." About 350 experts are participating to the Action, from 28 COST Countries (Austria, Belgium, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Ireland, Italy, Latvia, Malta, Macedonia, The Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey, United Kingdom), and from Albania, Armenia, Australia, Colombia, Egypt, Hong Kong, Jordan, Israel, Philippines, Russia, Rwanda, Ukraine, and United States of America. In September 2014, TU1208 has been recognised among the running Actions as "COST Success Story" ("The Cities of Tomorrow: The Challenges of Horizon 2020," September 17-19, 2014, Torino, IT - A COST strategic workshop on the development and needs of the European cities). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Moreover, the Action is oriented to the following specific objectives and expected deliverables: (i) coordinating European scientists to highlight problems, merits and limits of current GPR systems; (ii) developing innovative protocols and guidelines, which will be published in a handbook and constitute a basis for European standards, for an effective GPR application in civil- engineering tasks; safety, economic and financial criteria will be integrated within the protocols; (iii) integrating competences for the improvement and merging of electromagnetic scattering techniques and of data- processing techniques; this will lead to a novel freeware tool for the localization of

  20. Genetic dissection of the resistance to nine anthracnose races in the common bean differential cultivars MDRK and TU.

    Science.gov (United States)

    Campa, Ana; Giraldez, Ramón; Ferreira, Juan José

    2009-06-01

    Resistance to nine races of the pathogenic fungus Colletotrichum lindemuthianum, causal agent of anthracnose, was evaluated in F(3) families derived from the cross between the anthracnose differential bean cultivars TU (resistant to races, 3, 6, 7, 31, 38, 39, 102, and 449) and MDRK (resistant to races, 449, and 1545). Molecular marker analyses were carried out in the F(2) individuals in order to map and characterize the anthracnose resistance genes or gene clusters present in these two differential cultivars. The results of the combined segregation indicate that at least three independent loci conferring resistance to anthracnose are present in TU. One of them, corresponding to the previously described anthracnose resistance locus Co-5, is located in linkage group B7, and is formed by a cluster of different genes conferring specific resistance to races, 3, 6, 7, 31, 38, 39, 102, and 449. Evidence of intra-cluster recombination between these specific resistance genes was found. The second locus present in TU confers specific resistance to races 31 and 102, and the third locus confers specific resistance to race 102, the location of these two loci remains unknown. The resistance to race 1545 present in MDRK is due to two independent dominant genes. The results of the combined segregation of two F(4) families showing monogenic segregation for resistance to race 1545 indicates that one of these two genes is linked to marker OF10(530), located in linkage group B1, and corresponds to the previously described anthracnose resistance locus Co-1. The second gene conferring resistance to race 1545 in MDRK is linked to marker Pv-ctt001, located in linkage group B4, and corresponds to the Co-3/Co-9 cluster. The resistance to race 449 present in MDRK is conferred by a single gene, located in linkage group B4, probably included in the same Co-3/Co-9 cluster.

  1. COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar:" ongoing research activities and mid-term results

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Slob, Evert; Tosti, Fabio

    2015-04-01

    This work aims at presenting the ongoing activities and mid-term results of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar.' Almost three hundreds experts are participating to the Action, from 28 COST Countries (Austria, Belgium, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Ireland, Italy, Latvia, Malta, Macedonia, The Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey, United Kingdom), and from Albania, Armenia, Australia, Egypt, Hong Kong, Jordan, Israel, Philippines, Russia, Rwanda, Ukraine, and United States of America. In September 2014, TU1208 has been praised among the running Actions as 'COST Success Story' ('The Cities of Tomorrow: The Challenges of Horizon 2020,' September 17-19, 2014, Torino, IT - A COST strategic workshop on the development and needs of the European cities). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Moreover, the Action is oriented to the following specific objectives and expected deliverables: (i) coordinating European scientists to highlight problems, merits and limits of current GPR systems; (ii) developing innovative protocols and guidelines, which will be published in a handbook and constitute a basis for European standards, for an effective GPR application in civil- engineering tasks; safety, economic and financial criteria will be integrated within the protocols; (iii) integrating competences for the improvement and merging of electromagnetic scattering techniques and of data- processing techniques; this will lead to a novel freeware tool for the localization of buried objects

  2. Overview and comparative study of GPR international standards and guidelines - COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Marciniak, Marian; Benedetto, Andrea; Tosti, Fabio

    2016-04-01

    Ground Penetrating Radar (GPR) can be effectively used for non-destructive testing of composite structures and diagnostics affecting the whole life-cycle of civil engineering works. Nevertheless, few recognised international standards exist in this field and inhomogeneous recommendations are present in different countries. Moreover, the levels of knowledge, awareness and experience regarding the use of GPR in civil engineering vary strongly across different European areas. The COST Action TU1208 is working hard on leveraging these differences, by sharing and disseminating knowledge and experience, as well as by developing guidelines and protocols for a safe and effective use of GPR in civil engineering. GPR users need to know which is the best way to conduct GPR measurements and what the quality level for the results should be. The TU1208 guidelines will ensure a higher efficiency and quality of GPR services and they will constitute a scientific basis for the introduction of European Standards on the application of GPR in civil engineering. The aim of this contribution is to present an in-depth overview and critical analysis of the existing GPR international and national standards and guidelines. The main documents considered in our work are listed and briefly described in the following. Three standards are provided by the American Society for Testing and Materials (ASTM), to guide the GPR use for subsurface investigation, evaluation of asphalt-covered concrete bridge decks, and determination of pavement-layer thickness: 1. ASTM D6432-11, Standard Guide for Using the Surface Ground Penetrating Radar Method for Subsurface Investigation, ASTM International, West Conshohocken, PA, 2011, www.astm.org, DOI: 10.1520/D6432-11. 2. ASTM D6087-08, Standard Test Method for Evaluating Asphalt-Covered Concrete Bridge Decks Using Ground Penetrating Radar, ASTM International, West Conshohocken, PA, 2008, www.astm.org, DOI: 10.1520/D6087-08. 3. ASTM D4748-10, Standard Test Method

  3. High contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed Multispectral Optoacoustic Tomography (tuMSOT)

    CERN Document Server

    Stiel, Andre C; Jiang, Yuanyuan; Ntziachristos, Vasilis; Razansky, Daniel; Westmeyer, Gil G

    2014-01-01

    Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program optoacoustic signal time courses that were temporally unmixed to increase the proteins contrast-to-noise-ratios (CNRs) in optoacoustic imaging. In this way, two variants of the RSFP Dronpa with very similar optoacoustic spectra could be readily discriminated in the presence of highly absorbing blood. Addition of temporal unmixing to multispectral optoacoustic tomography (tuMSOT) in conjunction with synthetic or genetically controlled photochromic contrast agents and customized photoswitching schedules can increase the performance of multiplexed and high contrast molecular optoacoustic imaging.

  4. Control de criaderos de Aedes aegypticon el programa Recicla por tu bienestar en Mérida, México

    OpenAIRE

    Mario A. Barrera-Pérez; Norma Pavía-Ruz; Jorge Eduardo Mendoza-Mézquita; Nerio Torres-Arcila; Ramón Hernández-Hernández; Francisco Castro-Gamboa; Eduardo Geded-Moreno; Azael Cohuo-Rodríguez; Anuar Medina-Barreiro; Edgar Koyoc-Cardeña; Héctor Gómez-Dantés; Axel Kroeger; Gonzalo Vázquez-Prokopec; Pablo Manrique-Saide

    2015-01-01

    Objetivos. Determinar la importancia de los criaderos de Ae. aegypti en Mérida; evaluar el impacto del programa Recicla por tu bienestar (RxB) sobre la presencia/abundancia de éstos y la percepción de los habitantes. Material y métodos. S e calculó la importancia de los criaderos por su productividad pupal. Se realizaron muestreos pre y post RxB en colonias para cuantificar el total de recipientes/criaderos. Se aplicó una encuesta a participantes sobre la percepción sobre RxB en colonias sel...

  5. Caracterização da Liderança e Coesão nas Subunidades do Centro de Tropas Comandos durante a Preparação e Projeção de uma Força Nacional Destacada

    OpenAIRE

    Ramos, André

    2014-01-01

    O presente Trabalho de Investigação Aplicada incidiu sobre a “Caracterização da Liderança e Coesão nas Subunidades do Centro de Tropas Comandos durante a Preparação e Projeção de uma Força Nacional Destacada”. Teve como objetivo principal identificar os comportamentos de liderança que promovem níveis mais elevados de coesão nos pequenos grupos durante as fases de preparação, durante e no fim da missão de uma Força Nacional Destacada – Comandos. Para o estudo foi aplicad...

  6. Elongation Factor Tu and Heat Shock Protein 70 Are Membrane-Associated Proteins from Mycoplasma ovipneumoniae Capable of Inducing Strong Immune Response in Mice.

    Science.gov (United States)

    Jiang, Fei; He, Jinyan; Navarro-Alvarez, Nalu; Xu, Jian; Li, Xia; Li, Peng; Wu, Wenxue

    2016-01-01

    Chronic non-progressive pneumonia, a disease that has become a worldwide epidemic has caused considerable loss to sheep industry. Mycoplasma ovipneumoniae (M. ovipneumoniae) is the causative agent of interstitial pneumonia in sheep, goat and bighorn. We here have identified by immunogold and immunoblotting that elongation factor Tu (EF-Tu) and heat shock protein 70 (HSP 70) are membrane-associated proteins on M. ovipneumonaiea. We have evaluated the humoral and cellular immune responses in vivo by immunizing BALB/c mice with both purified recombinant proteins rEF-Tu and rHSP70. The sera of both rEF-Tu and rHSP70 treated BALB/c mice demonstrated increased levels of IgG, IFN-γ, TNF-α, IL-12(p70), IL-4, IL-5 and IL-6. In addition, ELISPOT assay showed significant increase in IFN-γ+ secreting lymphocytes in the rHSP70 group when compared to other groups. Collectively our study reveals that rHSP70 induces a significantly better cellular immune response in mice, and may act as a Th1 cytokine-like adjuvant in immune response induction. Finally, growth inhibition test (GIT) of M. ovipneumoniae strain Y98 showed that sera from rHSP70 or rEF-Tu-immunized mice inhibited in vitro growth of M. ovipneumoniae. Our data strongly suggest that EF-Tu and HSP70 of M. ovipneumoniae are membrane-associated proteins capable of inducing antibody production, and cytokine secretion. Therefore, these two proteins may be potential candidates for vaccine development against M. ovipneumoniae infection in sheep.

  7. Identification, genotypic relation, and clinical features of colistin-resistant isolates of Acinetobacter genomic species 13BJ/14TU from bloodstreams of patients in a university hospital.

    Science.gov (United States)

    Lee, Seung Yeob; Shin, Jong Hee; Park, Kyung Hwa; Kim, Ju Hee; Shin, Myung Geun; Suh, Soon Pal; Ryang, Dong Wook; Kim, Soo Hyun

    2014-03-01

    Colistin resistance remains rare among clinical isolates of Acinetobacter species. We noted the emergence of colistin-resistant bloodstream isolates of the Acinetobacter genomic species (GS) 13BJ/14TU from patients at a university hospital between 2003 and 2011. We report here, for the first time, the microbiological and molecular characteristics of these isolates, with clinical features of Acinetobacter GS 13BJ/14TU bacteremia. All 11 available patient isolates were correctly identified as Acinetobacter GS 13BJ/14TU using partial rpoB gene sequencing but were misidentified using the phenotypic methods Vitek 2 (mostly as Acinetobacter baumannii), MicroScan (mostly as A. baumannii/Acinetobacter haemolyticus), and the API 20 NE system (all as A. haemolyticus). Most isolates were susceptible to commonly used antibiotics, including carbapenems, but all were resistant to colistin, for which it is unknown whether the resistance is acquired or intrinsic. However, the fact that none of the patients had a history of colistin therapy strongly suggests that Acinetobacter GS 13BJ/14TU is innately resistant to colistin. The phylogenetic tree of multilocus sequence typing (MLST) showed that all 11 isolates formed a separate cluster from other Acinetobacter species and yielded five sequence types. However, pulsed-field gel electrophoresis (PFGE) revealed 11 distinct patterns, suggesting that the bacteremia had occurred sporadically. Four patients showed persistent bacteremia (6 to 17 days), and all 11 patients had excellent outcomes with cleared bacteremia, suggesting that patients with Acinetobacter GS 13BJ/14TU-associated bacteremia show a favorable outcome. These results emphasize the importance of precise species identification, especially regarding colistin resistance in Acinetobacter species. In addition, MLST offers another approach to the identification of Acinetobacter GS 13BJ/14TU, whereas PFGE is useful for genotyping for this species.

  8. Conceitualização pelas enfermeiras de preparação para o parto Conceptualización de los enfermeros de preparación para el parto Nurses' conceptualization on childbirth preparation

    Directory of Open Access Journals (Sweden)

    Germano Rodrigues Couto

    2006-04-01

    Full Text Available Estudo descritivo, exploratório e qualitativo, realizado mediante a utilização da Técnica de Delphi com amostra de 32 enfermeiras especialistas em Enfermagem de Saúde Materna e Obstétrica que exercem funções em 3 hospitais e 9 centros de saúde do distrito do Porto, norte de Portugal. Teve como objetivos: 1. identificar as idéias e concepções consensuais que um grupo de enfermeiras possui sobre a Preparação para o Parto; 2. saber quais as fontes de informação que consensualmente são mais utilizadas pelas enfermeiras; 3. saber qual a sua aceitação acerca de programas sobre Preparação para o Parto como forma da grávida ter um trabalho de parto ativo. Os resultados revelaram que a Preparação para o Parto é, segundo as enfermeiras estudadas, um momento de educação para a saúde, que envolve procedimentos técnicos, educacionais, relacionais e informativos; de grande importância para a grávida e para a enfermeira, desenrolando-se desde o início da gravidez até o puerpério, como um meio de alterar comportamentos errados com vistas a um resultado esperado com ganhos de saúde para a grávida e sua família.Estudio descriptivo, exploratorio y cualitativo, realizado mediante la utilización de la Técnica de Delphi a una muestra de 32 enfermeras especialistas en Enfermería de Salud Materna y Obstetricia que ejercen funciones en 3 hospitales y 9 centros de Atención Primaria del distrito de Oporto, al norte de Portugal. Tuvo como objetivos: 1. identificar las ideas y concepciones que un grupo de enfermeras posee sobre la Preparación para el Parto; 2. saber cuales son las fuentes de información mas utilizadas por las enfermeras; 3. saber cual es su aceptación sobre programas de Preparación para el Parto y como ayudar a la embarazada en el trabajo de parto. Los resultados revelaron que la Preparación para el Parto es, según las enfermeras de este estudio, un momento de educación para la salud, que envuelve

  9. Meta-heurística para programação da produção com tempos de preparação dependentes da seqüência Metaheuristic for scheduling with dependent setup times

    Directory of Open Access Journals (Sweden)

    Hamilton Carlos Massaro Santos

    1995-12-01

    Full Text Available Este trabalho considera o problema de programação da produção, em uma máquina, de um conjunto de ordens de produção que podem ser agrupadas em famílias, sendo que os tempos de preparação entre essas famílias são dependentes da seqüência em que são executadas. Propõe-se um procedimento aproximado, baseado na meta-heurística de Busca Tabu, para a resolução deste problema. A função objetivo considera uma ponderação envolvendo os custos de preparação de máquina, uma penalidade por atraso em relação à data de entrega das ordens e o custo de estoque. O desempenho do método proposto é avaliado, computacionalmente, frente a três diferentes situações. 1 análise empírica de desempenho da heurística, em função dos parâmetros do problema; 2 comparação entre a heurística e regras de despacho tradicionais EDD e SPT; 3 emprego da heurística para a resolução de um problema prático real.This article focuses on the one machine scheduling problem where jobs can be grouped in classes with the same machine setups. The setup times between classes are sequence dependent. An approximation method based on Tabu Search metaheuristic is proposed. The objetive is to minimize the weighted sum of setup costs, tardiness and inventory holding costs. The performance of the heuristic is evaluated through three sets of computational tests: 1 empirical performance analysis of the heuristic with different data sets; 2 comparison between the heuristic and the well known dispatching rules EDD and SPT; 3 application of the heuristic for solving a real life scheduling problem.

  10. Mood changes in the course of preparation for the Brazilian university admission exam - a longitudinal study Alterações de humor ao longo da preparação para o vestibular - um estudo longitudinal

    Directory of Open Access Journals (Sweden)

    Marco Aurélio Monteiro Peluso

    2010-03-01

    Full Text Available OBJECTIVE: Academic examinations are an important source of stress for students. This study is intended to assess senior high school students' mood changes during the preparation for a very important academic examination, the Brazilian university admission examination. METHOD: Positive and Negative Affect Schedule - Expanded Form was used to assess the students three times in the course of the months preceding the examination. RESULTS: ANOVA for repeated measures showed that Negative Affect increased as the examination approached, and that this increment was already present months before the examinationtookplace. Asingle conditionallyindependent random effect model showed that time spent studying was associated with fluctuations in mood. CONCLUSION: Teenagers, in preparation for a very important examination, may present mood disturbances associated with both the proximity of the examination and their study load months before the examination actually takes place. Other stressing factors may play a role in these findings and should be further investigated.OBJETIVO: Exames acadêmicos são uma fonte importante de estresse para estudantes. Este estudo teve como objetivo avaliar alterações de humor em estudantes de terceiro ano do Ensino Médio ao longo da preparação para um exame acadêmico de grande importância, o vestibular. MÉTODO: Positive and Negative Affect Schedule - Expanded Form foi utilizado para avaliar os estudantes três vezes ao longo dos meses antecedendo o exame. RESULTADOS: ANOVA para medidas repetidas mostrou que o afeto negativo aumentou com a aproximação do exame e que esse aumento já estava presente meses antes dele acontecer. Um modelo de efeitos aleatórios com independência condicional mostrou que o tempo de estudo estava associado às flutuações de humor. CONCLUSÃO: Adolescentes em preparação para exames muito importantes podem apresentar um distúrbio de humor associado à proximidade do exame e à quantidade

  11. Os sentidos e destinos do cuidar na preparação dos cuidadores de idosos The meanings and destinies of caregiving in the preparation of caregivers for the old-aged

    Directory of Open Access Journals (Sweden)

    Virgínia Lúcia Reis Maffioletti

    2006-12-01

    Full Text Available Neste trabalho apresentamos alguns pressupostos teóricos que informam a preparação de cuidadores de idosos, proposta por dois cursos realizados na cidade do Rio de Janeiro. Utilizamos uma metodologia qualitativa adotando como instrumentos de coleta de dados a observação participante e gravação das aulas, entrevistas com os coordenadores e, como fontes documentais, os programas, apostilas e referências bibliográficas. Os dados, ordenados em categorias descritivas, foram analisados à luz da literatura. Concluímos com uma reflexão acerca dos pressupostos que têm referenciado a preparação dos cuidadores de idosos e suas conseqüências para o cuidar, inclusive o debate entre estes e a enfermagem. Destacamos: a oposição entre cuidados invisíveis, cuidados visíveis e cuidados técnicos; a naturalização do cuidar como um valor em detrimento de um aprendizado técnico; e as concepções de cuidado social e cuidado com a saúde. Consideramos que tanto a ênfase no cuidado social, ao afastar a figura médica da doença, quanto a ênfase no cuidado à saúde, que tem a doença como mote reflexivo, ao desconsiderarem a importância do cuidado técnico, podem, com isso, não levar em consideração a complexidade dos aspectos subjetivos e físicos ligados à existência concreta do enfermo idoso.In this paper we present some theoretical presuppositions with respect to the qualification of old-age caregivers proposed by two courses of the city of Rio de Janeiro. We adopted a qualitative methodology, using as instruments for data collection direct observation, tape recording of the ministered classes and interviews with the course managers. Documental sources were the course programs, texts and lists of source materials. The data were ordered in descriptive categories that were compared with the literature. We emphasize: the difference between invisible care, visible care and technical care; the idea of care as a natural value with no need

  12. Nondestructive tests for railway monitoring. European Experience in COST Action TU1208

    Science.gov (United States)

    Fontul, Simona; Solla, Mercedes; Loizos, Andreas

    2016-04-01

    The railway monitoring is an important issue for a proper maintenance planning. With the increase in loads and travel speed, it is important to be able to diagnose the track defects and to plan the proper maintenance without interfering with the users. Traditionally, the maintenance actions are planned based on the geometric level parameters assessed without contact with the line, at traffic speed, by dedicated inspection vehicles. Nevertheless, the geometric condition of the line does not provide information on the defects causes. In order to complements the information on the causes, geophysics measurements can be performed in a nondestructive way. Among these later methods, Ground Penetrating Radar (GPR) is a quick and effective technique to evaluate infrastructure condition in a continuous manner, replacing or reducing the use of traditional drilling method. GPR application to railways infrastructures, during construction and monitoring phase, is relatively recent. It is based on the measuring of layers thicknesses and detection of structural changes. It also enables the assessment of materials properties that constitute the infrastructure and the evaluation of the different types of defects such as ballast pockets, fouled ballast, poor drainage, subgrade settlement and transitions problems. These deteriorations are generally the causes of vertical deviations in track geometry. Moreover, the development of new GPR systems with higher antenna frequencies, better data acquisition systems, more user friendly software and new algorithms for calculation of materials properties can lead to a regular use of GPR. A resume of the European experience in COST Action TU1208 of the application of GPR for railway monitoring and the measurement interpretation is presented in this paper. Also complementary nondestructive tests and other geophysical methods are referred, together with case studies of their application. The main troubleshooting and the needs for data analysis

  13. Reducing ppGpp level rescues an extreme growth defect caused by mutant EF-Tu.

    Science.gov (United States)

    Bergman, Jessica M; Hammarlöf, Disa L; Hughes, Diarmaid

    2014-01-01

    Transcription and translation of mRNA's are coordinated processes in bacteria. We have previously shown that a mutant form of EF-Tu (Gln125Arg) in Salmonella Typhimurium with a reduced affinity for aa-tRNA, causes ribosome pausing, resulting in an increased rate of RNase E-mediated mRNA cleavage, causing extremely slow growth, even on rich medium. The slow growth phenotype is reversed by mutations that reduce RNase E activity. Here we asked whether the slow growth phenotype could be reversed by overexpression of a wild-type gene. We identified spoT (encoding ppGpp synthetase/hydrolase) as a gene that partially reversed the slow growth rate when overexpressed. We found that the slow-growing mutant had an abnormally high basal level of ppGpp that was reduced when spoT was overexpressed. Inactivating relA (encoding the ribosome-associated ppGpp synthetase) also reduced ppGpp levels and significantly increased growth rate. Because RelA responds specifically to deacylated tRNA in the ribosomal A-site this suggested that the tuf mutant had an increased level of deacylated tRNA relative to the wild-type. To test this hypothesis we measured the relative acylation levels of 4 families of tRNAs and found that proline isoacceptors were acylated at a lower level in the mutant strain relative to the wild-type. In addition, the level of the proS tRNA synthetase mRNA was significantly lower in the mutant strain. We suggest that an increased level of deacylated tRNA in the mutant strain stimulates RelA-mediated ppGpp production, causing changes in transcription pattern that are inappropriate for rich media conditions, and contributing to slow growth rate. Reducing ppGpp levels, by altering the activity of either SpoT or RelA, removes one cause of the slow growth and reveals the interconnectedness of intracellular regulatory mechanisms.

  14. Reducing ppGpp level rescues an extreme growth defect caused by mutant EF-Tu.

    Directory of Open Access Journals (Sweden)

    Jessica M Bergman

    Full Text Available Transcription and translation of mRNA's are coordinated processes in bacteria. We have previously shown that a mutant form of EF-Tu (Gln125Arg in Salmonella Typhimurium with a reduced affinity for aa-tRNA, causes ribosome pausing, resulting in an increased rate of RNase E-mediated mRNA cleavage, causing extremely slow growth, even on rich medium. The slow growth phenotype is reversed by mutations that reduce RNase E activity. Here we asked whether the slow growth phenotype could be reversed by overexpression of a wild-type gene. We identified spoT (encoding ppGpp synthetase/hydrolase as a gene that partially reversed the slow growth rate when overexpressed. We found that the slow-growing mutant had an abnormally high basal level of ppGpp that was reduced when spoT was overexpressed. Inactivating relA (encoding the ribosome-associated ppGpp synthetase also reduced ppGpp levels and significantly increased growth rate. Because RelA responds specifically to deacylated tRNA in the ribosomal A-site this suggested that the tuf mutant had an increased level of deacylated tRNA relative to the wild-type. To test this hypothesis we measured the relative acylation levels of 4 families of tRNAs and found that proline isoacceptors were acylated at a lower level in the mutant strain relative to the wild-type. In addition, the level of the proS tRNA synthetase mRNA was significantly lower in the mutant strain. We suggest that an increased level of deacylated tRNA in the mutant strain stimulates RelA-mediated ppGpp production, causing changes in transcription pattern that are inappropriate for rich media conditions, and contributing to slow growth rate. Reducing ppGpp levels, by altering the activity of either SpoT or RelA, removes one cause of the slow growth and reveals the interconnectedness of intracellular regulatory mechanisms.

  15. The Dynamic Atmospheres of Mira Stars: Comparing the CODEX Models to PTI Time Series Observations of TU Andromedae

    Science.gov (United States)

    Hillen, M.; Verhoelst, T.; Degroote, P.; Acke, B.; Van Winckel, H.

    2015-08-01

    We present our already-published evaluation of the effectiveness of the CODEX models, released in 2011, in representing the atmospheres of M-type Mira variables. We present a high-precision interferometric K-band time series of TU And, consisting of 50 nights that cover eight consecutive pulsation cycles. At each phase, the flux at 2.2μm was obtained, along with the spectral shape and visibility points in five channels across the K band. We show a comparison between these data and the dynamical self-excited CODEX model which gives the closest match in stellar parameters yet available. Both the spectrum and the visibilities are consistently reproduced around visual minimum phases. Near the maximum phases, however, the current models predict a photosphere that is too hot and compact, surrounded by an extended atmosphere that lacks H2O opacity, compared to the observations. A better coverage in the model parameter space is needed to make firm conclusions as to the cause of the discrepancies. In the case of TU And, the discrepancy might be lifted by adopting a lower value of the mixing length parameter combined with an increased stellar mass and/or a decreased metallicity.

  16. Vysílač sériových dat ve formátu RS-232

    OpenAIRE

    Paul, Martin

    2008-01-01

    Cílem této bakalářské práce je navržení vysílače sériových dat formátu RS-232 ve vývojovém prostředí Xilinx ISE WebPACK s možností nastavit detaily formátu vysílaných dat vstupními signály. Dále je nutné prozkoumat požadavky navrženého vysílače na spotřebu strukturních prvků v různých cílových obvodech PLD a na závěr provést ověření funkčnosti návrhu pomocí vývojového kitu. The aim of my bachelor thesis is to create an RS-232 transmitter in the Xilinx ISE WebPACK development system with th...

  17. COST Action TU1208 - Working Group 4 - Combined use of GPR and other NDT methods & GPR applications in geosciences

    Science.gov (United States)

    Pajewski, Lara; Solla, Mercedes; Fontul, Simona

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 4 "Different applications of GPR and other NDT technologies in civil engineering" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). The main objective of the Action TU1208, started in April 2013 and ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst promoting in Europe the effective use of this safe non-destructive technique. The Action involves more than 150 Institutions from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. WG4 deals with the use of GPR outside from the civil engineering area, namely in archaeological prospecting and cultural heritage diagnostics, agriculture and management of water resources, investigation of polluted industrial sites, non-destructive testing of living tree trunks, planetary exploration, demining, localization of people buried under avalanches and debris, and more. Furthermore, this WG studies the integration of GPR with other Non-Destructive Testing (NDT) methods. The most relevant achievements stemming from WG4 will be presented during the 2017 EGU GA. These are: (i) The collection of thorough information on the state-of-the-art, ongoing studies, problems and future research needs on the topics of interest for this WG; (ii) The performance of a plethora of interesting case studies in important sites all over Europe, including well-known historical places such as Stonehenge (United Kingdom), Carnuntum (Austria), the Wawel Cathedral (Cracow, Poland), the Tholos Tomb of Acharnon (Athens, Greece), the Łazienki Royal Palace (Warsaw, Poland), and more; (iii) WG4 contributed to the TU1208 Education Pack, an open educational package conceived to teach GPR in University

  18. COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar": first-year activities and results

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Loizos, Andreas; Slob, Evert; Tosti, Fabio

    2014-05-01

    This work aims at presenting the first-year activities and results of COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar". This Action was launched in April 2013 and will last four years. The principal aim of COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Moreover, the Action is oriented to the following specific objectives and expected deliverables: (i) coordinating European scientists to highlight problems, merits and limits of current GPR systems; (ii) developing innovative protocols and guidelines, which will be published in a handbook and constitute a basis for European standards, for an effective GPR application in civil- engineering tasks; safety, economic and financial criteria will be integrated within the protocols; (iii) integrating competences for the improvement and merging of electromagnetic scattering techniques and of data- processing techniques; this will lead to a novel freeware tool for the localization of buried objects, shape-reconstruction and estimation of geophysical parameters useful for civil engineering needs; (iv) networking for the design, realization and optimization of innovative GPR equipment; (v) comparing GPR with different NDT techniques, such as ultrasonic, radiographic, liquid-penetrant, magnetic-particle, acoustic-emission and eddy-current testing; (vi) comparing GPR technology and methodology used in civil engineering with those used in other fields; (vii) promotion of a more widespread, advanced and efficient use of GPR in civil engineering; and (viii) organization of a high-level modular training program for GPR European users. Four Working Groups (WGs) carry out the research activities. The first WG

  19. Moisture evaluation of wood material using GPR with WARR method - COST Action TU1208

    Science.gov (United States)

    Reci, Hamza; Sbart'i, Zoubir Mehdi; Pajewski, Lara; Marciniak, Marian

    2016-04-01

    This work deals with the study of the sensitivity of GPR electromagnetic waves to moisture variation in wood material in relation with the direction of fibers and polarization of Electromagnetic field. The relations between relative permittivity and moisture content and the amplitude attenuation with distance was a target study using the direct waves in Wide Angle Radar Reflection (WARR) configuration. Comparison of results measured with reflected waves and direct waves was of main importance since they have different behavior in relation with moisture variation, due to different path of propagation. This research activity has been carried out during one Short-Term Scientific Missions (STSM) funded by the COST (European Cooperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in November-December 2015. In context of durability evaluation of construction materials, several studies have been carried out by the I2M team, University of Bordeaux, using direct and reflected waves for the evaluation of water content on concrete and wood materials [1-3]. As related to the wood material there is one study carried out using the reflected waves on wood for different humidity and different wood samples, in all the direction of polarization using GPR technique ground coupled antenna at 1.5 GHz [3]. This work continued with different moisture content in order to study the behavior of direct waves as function of moisture. Results taken from those measurements are compared with them from Fixed Offset (reflected method) with one antenna (1.5GHz or 2.6GHz), realized from the previous studies from the I2M and already published [1-3]. The results taken from this work from the reflected waves, show that the effect of wood anisotropy is significant on the variation of relative permittivity with moisture content on wood sample and that is in good agreement with the previous results [3-6]. As related to the direct waves, a small

  20. Assessment of waterfront location in hardened concrete by GPR within COST Action TU1208

    Science.gov (United States)

    Rodríguez-Abad, Isabel; Klysz, Gilles; Balayssac, Jean Paul; Pajewski, Lara

    2016-04-01

    This work focuses on the analysis of the capability of Ground-Penetrating radar (GPR) technique for evaluating how the water penetrates into concrete samples by means of the assessment of the waterfront advance. Research activities have been carried out during a Short-Term Scientific Missions (STSMs) funded by the COST (European Cooperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" in November 2015. The evaluation of water penetrability is crucial in most building materials, such us concrete, since, water and aggressive chemical agents dissolved therein contribute to the deterioration of the material. A number of techniques have been developed to measure their advance in concrete. Although the most common method for measuring water content is the gravimetric method by observing the change in mass, this method has a large number of disadvantages. In this context, non-destructive techniques as GPR play an interesting role. In particular, the application of GPR in the building materials area is providing very promising and interesting results regarding the building materials characterization and especially concrete deterioration evaluation [1-3]. In addition, recent experimental studies highlight the strong relation between wave propagation parameters (velocity and energy level) and water content advance [4-5]. Water content has a decisive influence on dielectric properties and those might be assessed by the study of the wave properties that are derived by using GPR. Therefore, the waterfront advance will result in a change on wave parameters. In line with this, this research is focused on the development of specific processing algorithms necessary to understand how the water penetrates and how the wave parameters will be affected regarding the location of the antenna in reference to the water absorption direction. For this purpose, concrete samples were manufactured, which after curing (90 days) and oven

  1. Keeleuuendus ja tõlkimine Noor-Eesti kultuurilise utoopia raames : Villem Ridala tõlgitud "Süütu" näitel / Daniele Monticelli

    Index Scriptorium Estoniae

    Monticelli, Daniele

    2006-01-01

    Keeleuuenduse ja tõlketegevuse mõningastest olulistest seostest Noor-Eesti liikumisega alanud kultuurilise revolutsiooni kontekstis. Lähema lingvistilise vaatluse alla võetakse Gabriele D'Annunzio "L'Innocente" ("Süütu") Villem Ridala tõlkes. Järgneb: Keel ja Kirjandus, 2006, nr. 6, lk. 477-490

  2. Effect of thiostrepton and 3'-terminal fragments of aminoacyl-tRNA on EF-Tu and ribosome-dependent GTP hydrolysis.

    Science.gov (United States)

    Bhuta, P; Chládek, S

    1982-08-30

    The effect of the antibiotics thiostrepton and micrococcin on EF-Tu-catalyzed (ribosome-dependent) GTP hydrolysis in the presence of A-Phe, C-A-Phe, or C-C-A-Phe (related to the sequence of the 3'-terminus of aminoacyl-tRNA)(System I) or by methanol ('uncoupled GTPase', System II) was investigated. In System I, thiostrepton increases the binding affinities of the effectors to the EF-Tu.GTP.70 S ribosome complex, as well as the extent of the GTP hydrolysis, while the KmGTP is virtually unchanged. Similarly, in the uncoupled system (System II) and in the absence of effectors, thiostrepton significantly increases VmaxGTP, whereas KmGTP remains unaffected. Micrococcin is without any effect in both systems. The 'uncoupled GTPase' (in System II) is also strongly inhibited by C-A-Phe. The results indicate the crucial role of the EF-Tu site which binds the aminoacylated C-C-A terminus of aminoacyl-tRNA in promoting GTP hydrolysis. It follows that the binding of the model effectors (such as C-C-A-Phe) to that site is favorably influenced by the interaction of thiostrepton with the 50 S ribosomal subunit, whereas thiostrepton, per se, does not influence the affinity of EF-Tu for GTP.

  3. Institute of place making: A project by the chair of Landscape Architecture at the TU Delft. Oerol 2013: Sense of place

    NARCIS (Netherlands)

    Pouderoijen, M.T.; Piccinini, D.

    2013-01-01

    This booklet shows the results of a project developed by TU Delft in a Master elective course offered by the chair of Landscape Architecture: Landscape Architecture ON site, being part of Oerol Festival 2013. The aim of the project was to express the landscape of Terschelling into a temporarily proj

  4. El uso de drogas en acuacultura: ¿te dispones a aplicar una droga y/o químico en tu sistema de cultivo?

    OpenAIRE

    Montoya, Nelson

    2002-01-01

    El uso de drogas en acuacultura: ¿te dispones a aplicar una droga y/o químico en tu sistema de cultivo?. La aplicación de drogas no reguladas o no aprobadas para su uso en acuacultura representa un riesgo humano potencial.

  5. :Examination of Sulfate production by CB05TU, RACM2 & RACM2 with SCI initiated SO2,oxidation in the Northern Hemisphere"

    Science.gov (United States)

    We employ the Community Multiscale Air Quality model to examine tropospheric sulfateproduction in the northern hemisphere using the Carbon Bond 2005 chemical mechanism withupdated toluene chemistry (CB05TU) and the Regional Atmospheric Chemistry Mechanism(RACM2) without and with ...

  6. Investigation of functional aspects of the N-terminal region of elongation factor Tu from Escherichia coli using a protein engineering approach

    DEFF Research Database (Denmark)

    Laurberg, M; Mansilla, Francisco; Clark, Brian F. C.

    1998-01-01

    The function of the N-terminal region of elongation factor Tu is still unexplained. Until recently, it has not been visible in electron density maps from x-ray crystallography studies, but the presence of several well conserved basic residues suggest that this part of the molecule is of structura...

  7. Preparação psicológica e o estresse de crianças submetidas a cirurgias Preparación psicológica y el estrés de los niños sometidos a cirugía Psychological preparation and stress of children undergoing surgery

    Directory of Open Access Journals (Sweden)

    Camilla Volpato Broering

    2011-03-01

    Full Text Available Este artigo tem por objetivo apresentar uma pesquisa que avaliou os efeitos da preparação psicológica pré-cirúrgica sobre o estresse de crianças submetidas a cirurgias eletivas em que foram utilizados dois programas distintos de preparação. A pesquisa foi realizada com 30 participantes, divididos em dois grupos, usuários de um hospital infantil, e nela utilizou-se a Escala de Estresse Infantil (ESI. A pesquisa compreendeu três etapas distintas: a aplicação da ESI antes da preparação, no dia anterior à cirurgia; b a preparação propriamente dita, sendo ambos os grupos submetidos aos diferentes programas de preparação; e c a reaplicação da ESI, depois da preparação. Os resultados mostraram que há significativa redução do nível de estresse depois da preparação, embora não tenha havido diferença significativa quanto ao tipo de programa utilizado. Há implicações práticas no atendimento psicológico das crianças em situação pré-cirúrgica e limitações a serem consideradas quando se pesquisa esta área da psicologia pediátrica.Este artículo tiene como objetivo presentar un estudio que evaluó los efectos de la preparación psicológica pre-quirúrgica sobre el estrés en niños sometidos a cirugía electiva, con dos programas de preparación. La investigación se llevó a cabo con 30 participantes, divididos en dos grupos, los usuarios de un hospital de niños y se utiliza la Escala de Estrés infantil (ESI. Hubo tres fases distintas: a Aplicación de ESI antes de la preparación, el día antes de la cirugía, b la preparación en sí, en ambos grupos presentaron a diferentes programas de preparación, y c una reaplicación de ESI, después de la preparación. Los resultados mostraron que la reducción significativa en el nivel de estrés después de la preparación, aunque no hubo diferencias significativas en el tipo de programa utilizado. Hay implicaciones prácticas para la situación psicológica de los ni

  8. 地球信息图谱与数字地球%On Geo-informatic TuPu and Digital Earth

    Institute of Scientific and Technical Information of China (English)

    廖克; 秦建新; 张青年

    2001-01-01

    Digital Earth can be understood as digitized reoresentation and the recognition for real earth and related, geo-phenomena. Digital Earth consists of spatial information with different levels and application models with different objectives. It includes the establishment of base and thematic digital maps with global, national, regional and urban scales, and satellite image data with different resolution and various geo-informations such as population, economy, society and their application systems.   Geo-informatic TuPu, as graphic representation of spatio-temporal change of geo-information, is an effective tool for the application of Digital Earth. “Tu”, in Chinese word, includes graphics, maps and images. The Chinese word “Pu” means the well-regulated sequence arrangement of the congener things, such as spectrum, chromatogram. “TuPu” involves the characteristics of “Tu” and “Pu” at the same time.   Geo-information TuPu can not only reflect spatial distribution characteristics of geo-phenomena and regional differences between them, but also reveal the deeper level rules related to the cause of formation, the process of evolution and morphology. Having the aid of computer visualization and Virtual Reality technology, TuPu can dynamically and muti-dimensionally reveal the spatial-temporal distribution and evolvement laws of all kinds of geo-phenomena.   In this paper, the technological system of Digital Earth and its significance, and the concept of Geo-informatic TuPu are discussed firstly, then, the model of Geo-informatic TuPu transmission is presented. On the basis of the above-mentioned researches, the relationship between Digital Earth and Geo-informatic TuPu is discussed. It reaches to the conclusion that the development of Digital Earth will provide with abundant information resources and strong technological support for Geo-informatic TuPu researches, and the enforcement of Digital Earth strategy will supply Geo-informatic Tu

  9. Two-way WKB Approximation Applied to GPR - COST Action TU1208

    Science.gov (United States)

    Prokopovich, Igor; Popov, Alexei; Marciniak, Marian; Pajewski, Lara

    2016-04-01

    account. In this work we study bistatic EM pulse probing of a horizontally layered medium in a 2D case. Coupled WKB equations set describing both forward and backward waves are derived and solved analytically. The comparison of our semi-analytical solutions with numerical calculations by gprMax software [4] demonstrates a good agreement, being hundreds of times faster than the letter. Our numerical results explain the protracted return pulses in the low-frequency GPR data. As an example, we discuss the experimental data obtained during the GPR mission in search of a big fragment of Chelyabinsk meteorite under a thick silt layer at the bottom of Chebarcul' Lake [5]. Acknowledgement The Authors are grateful to the European Cooperation in Science and Technology (www.cost.eu) facilitating this work by a Short-Term Scientific Missions (STSM) within the framework of the Action TU1208 "Civil engineering applications of Ground Penetrating Radar" (www.GPRadar.eu). References 1. H. Bremmer "Propagation of electromagnetic waves", in Handbuch der Physik, S. Flugge, Ed. Berlin-Goettingen-Heidelberg: Springer, 1958, pp. 423-639 2. L.M. Brekhovskikh, Waves in Stratified Media (in Russian). Moscow: USSR Academy of Sciences, 1957. 3. V.A.Vinogradov, V.V. Kopeikin, A.V. Popov, "An Approximate Solution of 1D Inverse Problem", in Proc. 10th Internat. Conf. on GPR, 21-24 June, 2004, Delft, The Netherlands 4. A. Giannopoulos, "Modelling ground penetrating radar by GprMax", Construction and Building Materials, vol. 19, no. 10, pp. 755-762, 2005, doi: 10.1016/j.conbuildmat.2005.06.007 5. V. V. Kopeikin , V. D. Kuznetsov, P. A. Morozov, A. V. Popov et al., "Ground penetrating radar investigation of the supposed fall site of a fragment of the Chelyabinsk meteorite in Lake Chebarkul'", Geochemistry International, vol. 51, no. 7, pp. 575-582, 2013, doi: 10.1134/S0016702913070112

  10. Transgenic Brassica rapa plants over-expressing eIF(iso)4E variants show broad-spectrum Turnip mosaic virus (TuMV) resistance.

    Science.gov (United States)

    Kim, Jinhee; Kang, Won-Hee; Hwang, Jeena; Yang, Hee-Bum; Dosun, Kim; Oh, Chang-Sik; Kang, Byoung-Cheorl

    2014-08-01

    The protein-protein interaction between VPg (viral protein genome-linked) of potyviruses and eIF4E (eukaryotic initiation factor 4E) or eIF(iso)4E of their host plants is a critical step in determining viral virulence. In this study, we evaluated the approach of engineering broad-spectrum resistance in Chinese cabbage (Brassica rapa) to Turnip mosaic virus (TuMV), which is one of the most important potyviruses, by a systematic knowledge-based approach to interrupt the interaction between TuMV VPg and B. rapa eIF(iso)4E. The seven amino acids in the cap-binding pocket of eIF(iso)4E were selected on the basis of other previous results and comparison of protein models of cap-binding pockets, and mutated. Yeast two-hybrid assay and co-immunoprecipitation analysis demonstrated that W95L, K150L and W95L/K150E amino acid mutations of B. rapa eIF(iso)4E interrupted its interaction with TuMV VPg. All eIF(iso)4E mutants were able to complement an eIF4E-knockout yeast strain, indicating that the mutated eIF(iso)4E proteins retained their function as a translational initiation factor. To determine whether these mutations could confer resistance, eIF(iso)4E W95L, W95L/K150E and eIF(iso)4E wild-type were over-expressed in a susceptible Chinese cabbage cultivar. Evaluation of the TuMV resistance of T1 and T2 transformants demonstrated that the over-expression of the eIF(iso)4E mutant forms can confer resistance to multiple TuMV strains. These data demonstrate the utility of knowledge-based approaches for the engineering of broad-spectrum resistance in Chinese cabbage.

  11. TU-100 (Daikenchuto and ginger ameliorate anti-CD3 antibody induced T cell-mediated murine enteritis: microbe-independent effects involving Akt and NF-κB suppression.

    Directory of Open Access Journals (Sweden)

    Nobuhiro Ueno

    Full Text Available The Japanese traditional medicine daikenchuto (TU-100 has anti-inflammatory activities, but the mechanisms remain incompletely understood. TU-100 includes ginger, ginseng, and Japanese pepper, each component possessing bioactive properties. The effects of TU-100 and individual components were investigated in a model of intestinal T lymphocyte activation using anti-CD3 antibody. To determine contribution of intestinal bacteria, specific pathogen free (SPF and germ free (GF mice were used. TU-100 or its components were delivered by diet or by gavage. Anti-CD3 antibody increased jejunal accumulation of fluid, increased TNFα, and induced intestinal epithelial apoptosis in both SPF and GF mice, which was blocked by either TU-100 or ginger, but not by ginseng or Japanese pepper. TU-100 and ginger also blocked anti-CD3-stimulated Akt and NF-κB activation. A co-culture system of colonic Caco2BBE and Jurkat-1 cells was used to examine T-lymphocyte/epithelial cells interactions. Jurkat-1 cells were stimulated with anti-CD3 to produce TNFα that activates epithelial cell NF-κB. TU-100 and ginger blocked anti-CD3 antibody activation of Akt in Jurkat cells, decreasing their TNFα production. Additionally, TU-100 and ginger alone blocked direct TNFα stimulation of Caco2BBE cells and decreased activation of caspase-3 and polyADP ribose. The present studies demonstrate a new anti-inflammatory action of TU-100 that is microbe-independent and due to its ginger component.

  12. A graduação em Psicologia prepara para o trabalho no hospital? ¿La graduación en Psicología prepara para el trabajo en el hospital? Does the undergraduation course in Psychology prepare to the work in a hospital?

    Directory of Open Access Journals (Sweden)

    Zeila Facci Torezan

    2013-01-01

    Full Text Available Considerando a Psicologia hospitalar uma especialidade em expansão no campo da Psicologia da saúde, o presente trabalho resulta de uma pesquisa exploratória sobre a preparação na graduação dos psicólogos hospitalares, que verificou a relação da formação acadêmica com a prática em hospitais gerais na região de Londrina - PR. Participaram desta pesquisa 10 psicólogos hospitalares com vínculo empregatício de no mínimo seis meses e três coordenadores de cursos de graduação em Psicologia de Londrina. Para a coleta dos dados, foram utilizados dois questionários de entrevista semiestruturada que continham questões abertas e fechadas. O primeiro questionário levantou dados sobre a formação acadêmica e as funções desenvolvidas pelos psicólogos nos hospitais, e o segundo buscou informações referentes às oportunidades de formação voltadas para a área de saúde e ofertadas pelos cursos de Psicologia pesquisados. Também foi realizada consulta aos projetos pedagógicos dos referidos cursos. No universo pesquisado, constatou-se que, em geral, a formação na graduação em Psicologia não contempla as particularidades necessárias para o trabalho em hospital geral.Considerando la Psicología hospitalaria una especialidad en expansión en el campo de la Psicología de la salud, el presente trabajo resulta de una investigación exploradora sobre la preparación en la graduación de los psicólogos hospitalarios, que verificó la relación de la formación académica con la práctica en hospitales generales en la región de Londrina - PR. Participaron de esta investigación 10 psicólogos hospitalarios con vinculación laboral de por lo menos seis meses y tres coordinadores de cursos de graduación en Psicología de Londrina. Para la recolección de los datos, se utilizaron dos cuestionarios de entrevista semiestructurada que contenían preguntas abiertas y cerradas. El primer cuestionario relevó datos sobre la formaci

  13. 3TU.Datacentrum serves data-curation needs of geodesy, hydrology, and climate science in the Netherlands

    Science.gov (United States)

    Princic, A.; Rombouts, J. P.; de Smaele, M. M. P.

    2012-04-01

    3TU.Datacentrum (datacentrum.3tu.nl) is a portal for science- and technology research data in the Netherlands. It serves data-curation needs of scientists in different disciplines of engineering and science varying from hydrology, water management, electron microscopy, to remote sensing. This data center provides long-term archive and permanent access to research data, it enables sharing and reuse of research data, and provides tools for citation of research datasets. The versatility of disciplines and versatility of needs is a challenge of the data center. The solutions offered differ in the functionality offered to the user. For a complex collection of simple datasets consisting of ongoing hydrological measurements from several project partners, using several instruments, on several locations, over a long period of time, there was a need for central storage in order to share the data and enable easy combination of data sets. The measurements are not repeatable and are also valuable for multidisciplinary research, which justified investment in the preservation and 'publishing' of the data. For a simple collection of complex datasets consisting of measurements of the meteorological radar, obtaining high resolution temporal and spatial precipitation data including those non-detectable by standard meteorological radar like drizzle, there was a need to store the data due to volume. These data come from a single instrument and from a single organization. Sharing with other scientists was difficult, which justified investment in the preservation and 'publishing' of the data. 3TU.Datacentrum offered solutions for both cases. Standardized solution to both collections is the data model: datasets, instruments, locations and time frames are all objects on their own, with own metadata and interconnected through rdf relationships. These relations are exploited in the user interface for navigation and inclusion, e.g. of instrument and location metadata in the display of

  14. COST Action TU1208 - Working Group 2 - GPR surveying of pavements, bridges, tunnels and buildings; underground utility and void sensing

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Derobert, Xavier; Fontul, Simona; Govedarica, Miro; Gregoire, Colette; Loizos, Andreas; Perez-Gracia, Vega; Plati, Christina; Ristic, Aleksandar; Tosti, Fabio; Van Geem, Carl

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 2 "GPR surveying of pavements, bridges, tunnels and buildings; underground utility and void sensing" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). The principal goal of the Action, started in April 2013 and ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst promoting throughout Europe the effective use of this safe non-destructive technique. The Action involves more than 300 Members from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. The most interesting achievements of WG2 include: 1. The state of the art on the use of GPR in civil engineering was composed and open issues were identified. The few existing international/national guidelines/protocols for GPR inspection in civil engineering were reviewed and discussed. Academic end-users, private companies and stakeholders presented their point of view and needs. 2. Guidelines for investigating flexible pavement by using GPR were prepared, with particular regard to layer-thickness assessment, moisture-content sensing, pavement-damage detection and classification, and other main GPR-based investigations in pavement engineering. 3. Guidelines for GPR sensing and mapping of underground utilities and voids were prepared, with a main focus on urban areas. 4. Guidelines for GPR assessment of concrete structures, with particular regard to inspections in bridges and tunnels, were prepared. 5. A report was composed, including a series of practical suggestions and very useful information to guide GPR users during building inspection. 6. WG2 Members carried out a plethora of case studies where GPR was used to survey roads, highways, airport runways, car

  15. The dynamic atmospheres of Mira stars: comparing the CODEX models to PTI time series of TU Andromedae

    Science.gov (United States)

    Hillen, M.; Verhoelst, T.; Degroote, P.; Acke, B.; van Winckel, H.

    2012-02-01

    Context. Our comprehension of stellar evolution on the AGB still faces many difficulties. To improve on this, a quantified understanding of large-amplitude pulsator atmospheres and interpretation in terms of their fundamental stellar parameters are essential. Aims: We wish to evaluate the effectiveness of the recently released CODEX dynamical model atmospheres in representing M-type Mira variables through a confrontation with the time-resolved spectro-photometric and interferometric PTI data set of TU And. Methods: We calibrated the interferometric K-band time series to high precision. This results in 50 nights of observations, covering 8 subsequent pulsation cycles. At each phase, the flux at 2.2 μm is obtained, along with the spectral shape and visibility points in 5 channels across the K-band. We compared the data set to the relevant dynamical, self-excited CODEX models. Results: Both spectrum and visibilities are consistently reproduced at visual minimum phases. Near maximum, our observations show that the current models predict a photosphere that is too compact and hot, and we find that the extended atmosphere lacks H2O opacity. Since coverage in model parameter space is currently poor, more models are needed to make firm conclusions on the cause of the discrepancies. We argue that for TU And, the discrepancy could be lifted by adopting a lower value of the mixing length parameter combined with an increase in the stellar mass and/or a decrease in metallicity, but this requires the release of an extended model grid. Figure 4 and Table 1 are available in electronic form at http://www.aanda.org

  16. Optimização da Técnica de Preparação de Amostras de Betões Refractários Auto-Escoantes de Alumina Sem Cimento Para Observação Microscópica

    OpenAIRE

    Silva, Abílio Pereira; Gomes, Ana Paula; Segadães, Ana Maria; Devezas, Tessaleno Campos

    2010-01-01

    A preparação de amostras para a observação de superfícies em microscopia requer um procedimento de corte, rectificação e polimento rigoroso que obedeça a requisitos sistemáticos de avaliação da qualidade superficial. Neste trabalho, um novo material constituído por 100% de alumina, mas de ingredientes (matriz e agregado) com características distintas obrigou, à implementação de uma nova receita de polimento e de realce dos contrastes entre os elementos estruturais pres...

  17. tu ša šarri. La « loi du roi »dans la Babylonie achéménide et séleucide dātu ša šarri, The “Law of the King” in Achaemenid and Seleucid Babylonia

    Directory of Open Access Journals (Sweden)

    Sophie Démare-Lafont

    2009-06-01

    Full Text Available Cet article examine le champ sémantique de l’expression dātu ša šarri, « loi du roi », formée sur un terme d’origine vieux-perse (data. La dizaine d’occurrences d’époques achéménide et séleucide montre que la « loi du roi »désigne un mode d’élaboration de la norme issu de la pratique judiciaire du souverain, progressivement compilée pour former un corps de règles invoquées dans les contrats. Ce procédé de création de la loi et de fabrication des recueils législatifs rappelle celui du rescrit romain.This article investigates the meaning of the formula dātu ša šarri, “law of the king”, using the akkadian dātu based on the old Persian word dāta. The ten Achaemenid and Seleucid occurrences of this formula suggest a process of creation of legal rules comparable to the Roman rescript: the legal standard dātu ša šarri derives from the judicial practice of the king, whose decisions were progressively compiled in order to create a body of regulations which were invoked in contracts.

  18. COST Action TU1208 - Working Group 3 - Electromagnetic modelling, inversion, imaging and data-processing techniques for Ground Penetrating Radar

    Science.gov (United States)

    Pajewski, Lara; Giannopoulos, Antonios; Sesnic, Silvestar; Randazzo, Andrea; Lambot, Sébastien; Benedetto, Francesco; Economou, Nikos

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 3 "Electromagnetic methods for near-field scattering problems by buried structures; data processing techniques" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). The main objective of the Action, started in April 2013 and ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst promoting in Europe the effective use of this safe non-destructive technique. The Action involves more than 150 Institutions from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. Among the most interesting achievements of WG3, we wish to mention the following ones: (i) A new open-source version of the finite-difference time-domain simulator gprMax was developed and released. The new gprMax is written in Python and includes many advanced features such as anisotropic and dispersive-material modelling, building of realistic heterogeneous objects with rough surfaces, built-in libraries of antenna models, optimisation of parameters based on Taguchi's method - and more. (ii) A new freeware CAD was developed and released, for the construction of two-dimensional gprMax models. This tool also includes scripts easing the execution of gprMax on multi-core machines or network of computers and scripts for a basic plotting of gprMax results. (iii) A series of interesting freeware codes were developed will be released by the end of the Action, implementing differential and integral forward-scattering methods, for the solution of simple electromagnetic problems by buried objects. (iv) An open database of synthetic and experimental GPR radargrams was created, in cooperation with WG2. The idea behind this initiative is to give researchers the

  19. COST Action TU1208 - Working Group 1 - Design and realisation of Ground Penetrating Radar equipment for civil engineering applications

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; D'Amico, Sebastiano; Ferrara, Vincenzo; Frezza, Fabrizio; Persico, Raffaele; Tosti, Fabio

    2017-04-01

    This work aims at presenting the main results achieved by Working Group (WG) 1 "Novel Ground Penetrating Radar instrumentation" of the COST (European COoperation in Science and Technology) Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.cost.eu, www.GPRadar.eu). The principal goal of the Action, which started in April 2013 and is ending in October 2017, is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar techniques in civil engineering, whilst promoting throughout Europe the effective use of this safe non-destructive technique. The Action involves more than 300 Members from 28 COST Countries, a Cooperating State, 6 Near Neighbour Countries and 6 International Partner Countries. The most interesting achievements of WG1 include: 1. The state of the art on GPR systems and antennas was composed; merits and limits of current GPR systems in civil engineering applications were highlighted and open issues were identified. 2. The Action investigated the new challenge of inferring mechanical (strength and deformation) properties of flexible pavement from electromagnetic data. A semi-empirical method was developed by an Italian research team and tested over an Italian test site: a good agreement was found between the values measured by using a light falling weight deflectometer (LFWD) and the values estimated by using the proposed semi-empirical method, thereby showing great promises for large-scale mechanical inspections of pavements using GPR. Subsequently, the method was tested on a real scale, on an Italian road in the countryside: again, a good agreement between LFWD and GPR data was achieved. As a third step, the method was tested at larger scale, over three different road sections within the districts of Madrid and Guadalajara, in Spain: GPR surveys were carried out at the speed of traffic for a total of 39 kilometers, approximately; results were collected by using different GPR antennas

  20. Incidence and remission of parasomnias among adolescent children in the Tucson Children’s Assessment of Sleep Apnea (TuCASA) Study

    OpenAIRE

    Furet O; Goodwin JL; Quan SF

    2011-01-01

    Background: Longitudinal assessments of parasomnias in the adolescent population are scarce. This analysis aims to identify the incidence and remission of parasomnias in the adolescent age group.Methods: The TuCASA study is a prospective cohort study that initially enrolled children between the ages of 6 and 11 years (Time 1) and subsequently re-studied them approximately 5 years later (Time 2). At both time points parents were asked to complete a comprehensive sleep habits questionnaire desi...

  1. Praćenje ostataka sulfonilurea u zemljištu u realnim uslovima primenom visoko-pritisne tečne hromatografije

    OpenAIRE

    Grahovac, Nada

    2016-01-01

    U okviru doktorske disertacije razvijena je brza i selektivna metoda zaodređivanje i praćenje ostataka herbicida na bazi sulfonilurea (nikosulfuron,oksasulfuron, tribenuron metil, tritosulfuron, triasulfuron, rimsulfuron iprosulfuron) u zemljištu primenom visoko pritisne tečne hromatografije saUV detektor sa nizom fotoosetljivih dioda (HPLC-UV-DAD). Razdvajanjesulfonilurea je izvedeno uz primenu Zorbax Eclipse XDB-C18 kolone. U ciljudefinisanja odgovarajućih uslova analize, ispitan je ...

  2. A great honor and a huge challenge for China: You-you TU getting the Nobel Prize in Physiology or Medicine.

    Science.gov (United States)

    Yuan, Da; Yang, Xue; Guo, Jun-Chao

    2016-05-01

    Public excitement over the award of the 2015 Nobel Prize in Physiology or Medicine to the Chinese medical scientist You-you TU for the discovery of a herbal anti-malarial, may mislead the Chinese people into believing that traditional Chinese herbal medi-cine can be used to cure all disease without any ad-verse effects. The aim of this paper is to explain the advantages and disadvantages of herbal traditional Chinese medicine (TCM) objectively.

  3. "Toi qui, de façon générale..." : propositions relatives et "tu" générique en samba leko

    OpenAIRE

    Fabre, Gwenaëlle

    2004-01-01

    A partir de récits techniques en samba leko, je cherche ici à rendre compte de constructions originales qui associent un "tu" ou un "nous" au personnage central de l'activité décrite. Sur le plan syntaxique, il s'agit d'énoncés complexes qui comportent une proposition principale, une proposition relative et un topique.

  4. Dgp71WD is required for the assembly of the acentrosomal Meiosis I spindle, and is not a general targeting factor for the γ-TuRC

    Directory of Open Access Journals (Sweden)

    Richard F. Reschen

    2012-03-01

    Dgp71WD/Nedd1 proteins are essential for mitotic spindle formation. In human cells, Nedd1 targets γ-tubulin to both centrosomes and spindles, but in other organisms the function of Dgp71WD/Nedd1 is less clear. In Drosophila cells, Dgp71WD plays a major part in targeting γ-tubulin to spindles, but not centrosomes, while in Xenopus egg extracts, Nedd1 acts as a more general microtubule (MT organiser that can function independently of γ-tubulin. The interpretation of these studies, however, is complicated by the fact that some residual Dgp71WD/Nedd1 is likely present in the cells/extracts analysed. Here we generate a Dgp71WD null mutant lacking all but the last 12 nucleotides of coding sequence. The complete loss of Dgp71WD has no quantifiable effect on γ-tubulin or Centrosomin recruitment to the centrosome in larval brain cells. The recruitment of γ-tubulin to spindle MTs, however, is severely impaired, and spindle MT density is reduced in a manner that is indistinguishable from cells lacking Augmin or γ-TuRC function. In contrast, the absence of Dgp71WD leads to defects in the assembly of the acentrosomal female Meiosis I spindle that are more severe than those seen in Augmin or γ-TuRC mutants, indicating that Dgp71WD has additional functions that are independent of these complexes in oocytes. Moreover, the localisation of bicoid RNA during oogenesis, which requires γ-TuRC function, is unperturbed in Dgp71WD120 mutants. Thus, Dgp71WD is not simply a general cofactor required for γ-TuRC and/or Augmin targeting, and it appears to have a crucial role independent of these complexes in the acentrosomal Meiosis I spindle.

  5. VizieR Online Data Catalog: TU UMa light curves and maxima, CL Aur minima (Liska+, 2016)

    Science.gov (United States)

    Liska, J.; Skarka, M.; Mikulasek, Z.; Zejda, M.; Chrastina, M.

    2016-02-01

    Differential photometry for RR Lyrae star TU UMa in the 1st and 2nd file. The measurements were obtained using 24-inch and 1-inch telescopes, respectively. The observations were performed at the Masaryk University Observatory in Brno (3 nights, 24-inch), and at the private observatory in Brno (16 nights, 1-inch) in the Czech Republic from December 2013 to June 2014. Observing equipments consisted of 24-inch Newtonian telescope (600/2780mm, diameter/focal length) and a Moravian Instruments CCD camera G2-4000 with Stromgren photometric filters vby, and of 1-inch refractor (a photographic lens Sonnar 4/135mm, lens focal ratio/focal length) and ATIK 16IC CCD camera with green photometric filter with similar throughput as the Johnson V filter. Exposures were v - 60s, b - 30s, y - 30s, green - 30s. For the small aperture telescope, five frames were combined to a single image to achieve a better signal-to-noise ratio. The time resolution of a such combined frame is about 170s. CCD images were calibrated in a standard way (dark frame and flat field corrections). The C-Munipack software (Motl 2009) was used for this processing as well as for differential photometry. The comparison star BD+30 2165 was the same for both instruments, but the control stars were BD+30 2164 (for the 24-inch telescope) and HD 99593 (for the 1-inch telescope). The 3rd file contains maxima timings of TU UMa adopted from the GEOS RR Lyr database, from the latest publications, together with maxima timings determined in our study. Times of maxima were calculated from our observations, sky-surveys data (Hipparcos, NSVS, Pi of the Sky, SuperWASP), photographic measurements (project DASCH), and from several published datasets, in which the maxima were omitted or badly determined - Boenigk (1958AcA.....8...13B), Liakos, Niarchos (2011IBVS.6099....1L, 2011IBVS.5990....1L), Liu, Janes (1989ApJS...69..593L), Preston et al. (1961ApJ...133..484P). The 4th file contains minima timings of eclipsing binary CL Aur

  6. I Passiuna tu Christù - Rito e teatro di una cantica popolare della Grecìa Salentina

    Directory of Open Access Journals (Sweden)

    Diana Costa

    2012-01-01

    Full Text Available Abstract – IT Questo articolo si basa sugli esiti di una ricerca di campo condotta nei paesi della Grecìa Salentina,una zona del basso Salento, dove si riscontra una peculiare modalità di esecuzione della cantica popolare grìca conosciuta come I Passiuna tu Christù (La Passione di Cristo. Attorno alla metà degli anni ´90 del ´900 la tradizione della cantica è stata oggetto di un prezioso recupero, fondato sulla memoria e disponibilità di alcuni anziani cantori, che l’ha riportata in vita dopo anni di silenzio. Lo studio si è quindi focalizzato sull'esecuzione di Zollino, uno dei comuni grecanico-salentini, dove opera l'associazione culturale "Bottega del Teatro" che per prima ha promosso il recupero di cui si è detto. Protagonista e interprete raffinato della versione zollinese è l'anziano cantore Antimo Pellegrino, la cui voce si fa teatro attraverso un uso sapiente del corpo fatto di gesti stilizzati, regolati da codici che consentono di comprendere il divenire performativo, ossia il testo in grìco, anche a chi non intende tale idioma. Abstract – EN This article is based on the results of a field research conducted in the countries of the Grecìa Salentina, an area of Salento, where there is a particular execution method of the folksong known as I Passiuna tu Christù (The Passion of the Christ. Around the mid-90s of the 20th century the tradition of this folksong has been the subject of a careful recovery, based on memory and availability of some older singers, who brought it back to life after years of silence. The study then focused on the Zollino’s execution, one of the grecanico-salentino’s common, in which works the cultural association "Bottega del Teatro", the first promoter of this recovery. The old singer Antimo Pellegrino is the player and interpreter of the Zollino’s refined version, whose voice becomes theater through a wise use of body made of stylized gestures, regulated by codes that allow you to

  7. GPR applications in civil engineering collaborating with a Company and preparation of material for the educational package to teach in Universities. STSM, COST Action TU1208

    Science.gov (United States)

    Sossa, Viviana; Assunção, Sonia; Pérez, Vega

    2017-04-01

    The STSM, Short-Term Missions, was carried out with the support of Murphy Survey company from Dublin - Ireland, was proposed for to Develop a module "GPR Applications in Archaeology" of the educational pack defined in the different meetings of the Cost Action TU 1208. The employ of GPR is mainly determined by the limits and uncertainties associated to this technique that affects to the measurements and the results. Private companies and end-users are highly interested in known the limits associated to particular cases and the advantages of the methodology compared to other studies. During this STSM, we plan to prepare a field survey, analyzing the possible problems and defining the objectives, and to acquire data to assess the structure. This data will be analyzed and processed in order to determine the limits of the technique in the particular case. The results will be compared to other surveys, in order to determine some clear restrictions that the company need to take into account in further works. Aknowledgments: This work has been partially funded by the EU COST Action TU1208, "Civil Engineering Applications of Ground Penetrating Radar", and it is a contribution to the working group 2.2 tasks in the COST Action TU1208.

  8. Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles.

    Science.gov (United States)

    Shiri, Mahdi; Navaei-Nigjeh, Mona; Baeeri, Maryam; Rahimifard, Mahban; Mahboudi, Hossein; Shahverdi, Ahmad Reza; Kebriaeezadeh, Abbas; Abdollahi, Mohammad

    Diazinon (DZ) is an organophosphorus insecticide that acts as an acetylcholinesterase inhibitor. It is important to note that it can induce oxidative stress, lipid peroxidation, diabetic disorders, and cytotoxicity. Magnesium oxide (MgO) and selenium nanoparticles (Se NPs) showed promising protection against oxidative stress, lipid peroxidation, cytotoxicity, and diabetic disorders. Therefore, this study was conducted to explore the possible protective mechanisms of MgO and Se NPs against DZ-induced cytotoxicity in PaTu cell line. Cytotoxicity of DZ, in the presence or absence of effective doses of MgO and Se NPs, was determined in human pancreatic cancer cell line (PaTu cells) after 24 hours of exposure by using mitochondrial activity and mitochondrial membrane potential assays. Then, the insulin, proinsulin, and C-peptide release; caspase-3 and -9 activities; and total thiol molecule levels were assessed. Determination of cell viability, including apoptotic and necrotic cells, was assessed via acridine orange/ethidium bromide double staining. Furthermore, expression of 15 genes associated with cell death/apoptosis in various phenomena was examined after 24 hours of contact with DZ and NPs by using real-time polymerase chain reaction. Compared to the individual cases, the group receiving the combination of MgO and Se NPs showed more beneficial effects in reducing the toxicity of DZ. Cotreatment of PaTu cell lines with MgO and Se NPs counteracts the toxicity of DZ on insulin-producing cells.

  9. Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

    Science.gov (United States)

    Shiri, Mahdi; Navaei-Nigjeh, Mona; Baeeri, Maryam; Rahimifard, Mahban; Mahboudi, Hossein; Shahverdi, Ahmad Reza; Kebriaeezadeh, Abbas; Abdollahi, Mohammad

    2016-01-01

    Diazinon (DZ) is an organophosphorus insecticide that acts as an acetylcholinesterase inhibitor. It is important to note that it can induce oxidative stress, lipid peroxidation, diabetic disorders, and cytotoxicity. Magnesium oxide (MgO) and selenium nanoparticles (Se NPs) showed promising protection against oxidative stress, lipid peroxidation, cytotoxicity, and diabetic disorders. Therefore, this study was conducted to explore the possible protective mechanisms of MgO and Se NPs against DZ-induced cytotoxicity in PaTu cell line. Cytotoxicity of DZ, in the presence or absence of effective doses of MgO and Se NPs, was determined in human pancreatic cancer cell line (PaTu cells) after 24 hours of exposure by using mitochondrial activity and mitochondrial membrane potential assays. Then, the insulin, proinsulin, and C-peptide release; caspase-3 and -9 activities; and total thiol molecule levels were assessed. Determination of cell viability, including apoptotic and necrotic cells, was assessed via acridine orange/ethidium bromide double staining. Furthermore, expression of 15 genes associated with cell death/apoptosis in various phenomena was examined after 24 hours of contact with DZ and NPs by using real-time polymerase chain reaction. Compared to the individual cases, the group receiving the combination of MgO and Se NPs showed more beneficial effects in reducing the toxicity of DZ. Cotreatment of PaTu cell lines with MgO and Se NPs counteracts the toxicity of DZ on insulin-producing cells. PMID:27920530

  10. Burtnieku novada nekroģeogrāfija. Atvērto kapsētu veidošanas prakse

    OpenAIRE

    Seļicka, Anita

    2012-01-01

    Maģistra darba „Burtnieku novada nekroģeogrāfija. Atvērto kapsētu veidošanas prakse” mērķis ir izvērtēt Burtnieku novada aktīvo kapsētu veidošanas praksi nekroģeogrāfijas koncepciju un pieeju kontekstā. Pētījuma teorētiskajā daļā tiek apskatīts nekroģeogrāfijas koncepts, dažādās kapsētas jēdziena uztveres un duālismi tajā. Darbā analizēti piecu Burtnieku novada kapsētu telpiskie elementi, to struktūras veidošanās, tiesiskais regulējums, iesaistīto personu un apkārtējās teritorijas konteksts k...

  11. Pilnīgi un daļēji ar stronciju aizvietotu peroksiapatītu sintēze un to analīze

    OpenAIRE

    Valkovska, Valda

    2016-01-01

    Pilnīgi un daļēji ar stronciju aizvietotu peroksiapatītu sintēze un to analīze. Valkovska V., darba vadītāja docente, Dr. chem. Osīte A. Bakalaura darbs, 42 lappuses, 19 attēli, 4 tabulas, 46 literatūras avoti, 5 pielikumi. Latviešu valodā. Darbā ir apkopota informācija par hidroksilapatītu un peroksiapatītu uzbūvi, īpašībām, izmantošanu, iegūšanu un analīzes metodēm. Salīdzinātas atšķirīgu sintēžu priekšrocības un trūkumi. Eksperimentālajā daļā veiktas pilnīgi un daļēji ar stronciju aizvieto...

  12. Incidence and Remission of Parasomnias among Adolescent Children in the Tucson Children's Assessment of Sleep Apnea (TuCASA) Study.

    Science.gov (United States)

    Furet, Oscar; Goodwin, James L; Quan, Stuart F

    2011-01-01

    BACKGROUND: Longitudinal assessments of parasomnias in the adolescent population are scarce. This analysis aims to identify the incidence and remission of parasomnias in the adolescent age group. METHODS: The TuCASA study is a prospective cohort study that initially enrolled children between the ages of 6 and 11 years (Time 1) and subsequently restudied them approximately 5 years later (Time 2). At both time points parents were asked to complete a comprehensive sleep habits questionnaire designed to assess the severity of sleep-related symptoms that included questions about enuresis (EN), sleep terrors (TR), sleep walking (SW) and sleep talking (ST). RESULTS: There were 350 children participating at Time 1 who were studied as adolescents at time 2. The mean interval between measurements was (4.6 years). The incidence of EN, TR, ST, and SW in these 10-18 year old children was 0.3%, 0.6%, 6.0% and 1.1% respectively. Remission rates were 70.8%, 100%, 64.8% and 50.0% respectively. CONCLUSIONS: The incidence rates of EN, TR, and SW were relatively low moving from childhood to adolescence while remission rates were high across all parasomnias.

  13. Control de criaderos de Aedes aegypti con el programa Recicla por tu bienestar en Mérida, México

    Directory of Open Access Journals (Sweden)

    Mario Barrera Pérez

    2015-05-01

    Full Text Available Objetivos. Determinar la importancia de los criaderos de Ae. aegypti en Mérida; evaluar el impacto del programa Recicla or tu bienestar (RxB sobre la presencia/abundancia de éstos y la percepción de los habitantes. Material y métodos. Se calculó la importancia de los criaderos por su productividad pupal. Se realizaron muestreos pre y post RxB en colonias para cuantificar el total de recipientes/criaderos. Se aplicó una encuesta a participantes sobre la percepción sobre RxB en colonias seleccionadas. Resultados. Los botes, cubetas y diversos objetos chicos fueron los criaderos más importantes. RxB tuvo un impacto significativo en la reducción del número de recipientes (IRR=0.74, en los recipientes positivos (IRR=0.33 y en la positividad de las viviendas para Ae. aegypti (OR=0.41. Todos los entrevistados opinaron que RxB es necesario la gran mayoría piensa que es útil. Conclusiones. RxB debe ser considerada una buena práctica para el control del vector del dengue.

  14. Increasing HIV-related knowledge, communication, and testing intentions among Latinos: Protege tu Familia: Hazte la Prueba.

    Science.gov (United States)

    Rios-Ellis, Britt; Espinoza, Lilia; Bird, Mara; Garcia, Melawhy; D'Anna, Laura Hoyt; Bellamy, Laura; Scolari, Rosana

    2010-08-01

    Latinos are less likely to be aware of their HIV seropositivity than African Americans and Whites. 'Protege tu Familia: Hazte la Prueba' is a culturally and linguistically-sensitive HIV/AIDS prevention and testing program targeting Latino families. Using community-based participatory research techniques, Spanish-speaking bicultural community health workers helped develop and then used an educational flip chart and materials to conduct outreach and HIV prevention education in diverse settings. The intervention was created to increase HIV/AIDS-related knowledge, to improve communication regarding sexual risk, and to augment intentions to use condoms and test for HIV. A secondary purpose was to decrease HIV-related stigma by improving knowledge about transmission and reducing homophobia. Participants demonstrated significant increases in HIV knowledge, intention to practice safer sex and communicate sexual risk to partner(s), and intention to test for HIV. Improvements were also found in self-reported comfort levels when interacting with and caring for the HIV positive, thus decreasing HIV/AIDS-related stigma.

  15. The dynamic atmospheres of Mira stars: comparing the CODEX models to PTI time series of TU And

    CERN Document Server

    Hillen, M; Degroote, P; Acke, B; van Winckel, H

    2012-01-01

    Our comprehension of stellar evolution on the AGB still faces many difficulties. To improve on this, a quantified understanding of large-amplitude pulsator atmospheres and interpretation in terms of their fundamental stellar parameters are essential. We wish to evaluate the effectiveness of the recently released CODEX dynamical model atmospheres in representing M-type Mira variables through a confrontation with the time-resolved spectro-photometric and interferometric PTI data set of TU And. We calibrated the interferometric K-band time series to high precision. This results in 50 nights of observations, covering 8 subsequent pulsation cycles. At each phase, the flux at 2.2$\\mu$m is obtained, along with the spectral shape and visibility points in 5 channels across the K-band. We compared the data set to the relevant dynamical, self-excited CODEX models. Both spectrum and visibilities are consistently reproduced at visual minimum phases. Near maximum, our observations show that the current models predict a pho...

  16. The nuclear inclusion a (NIa) protease of turnip mosaic virus (TuMV) cleaves amyloid-β.

    Science.gov (United States)

    Han, Hye-Eun; Sellamuthu, Saravanan; Shin, Bae Hyun; Lee, Yong Jae; Song, Sungmin; Seo, Ji-Seon; Baek, In-Sun; Bae, Jeomil; Kim, Hannah; Yoo, Yung Joon; Jung, Yong-Keun; Song, Woo Keun; Han, Pyung-Lim; Park, Woo Jin

    2010-12-20

    The nuclear inclusion a (NIa) protease of turnip mosaic virus (TuMV) is responsible for the processing of the viral polyprotein into functional proteins. NIa was previously shown to possess a relatively strict substrate specificity with a preference for Val-Xaa-His-Gln↓, with the scissile bond located after Gln. The presence of the same consensus sequence, Val(12)-His-His-Gln(15), near the presumptive α-secretase cleavage site of the amyloid-β (Aβ) peptide led us to hypothesize that NIa could possess activity against Aβ. Western blotting results showed that oligomeric as well as monomeric forms of Aβ can be degraded by NIa in vitro. The specific cleavage of Aβ was further confirmed by mass spectrometry analysis. NIa was shown to exist predominantly in the cytoplasm as observed by immunofluorescence microscopy. The overexpression of NIa in B103 neuroblastoma cells resulted in a significant reduction in cell death caused by both intracellularly generated and exogenously added Aβ. Moreover, lentiviral-mediated expression of NIa in APP(sw)/PS1 transgenic mice significantly reduced the levels of Aβ and plaques in the brain. These results indicate that the degradation of Aβ in the cytoplasm could be a novel strategy to control the levels of Aβ, plaque formation, and the associated cell death.

  17. The nuclear inclusion a (NIa protease of turnip mosaic virus (TuMV cleaves amyloid-β.

    Directory of Open Access Journals (Sweden)

    Hye-Eun Han

    Full Text Available BACKGROUND: The nuclear inclusion a (NIa protease of turnip mosaic virus (TuMV is responsible for the processing of the viral polyprotein into functional proteins. NIa was previously shown to possess a relatively strict substrate specificity with a preference for Val-Xaa-His-Gln↓, with the scissile bond located after Gln. The presence of the same consensus sequence, Val(12-His-His-Gln(15, near the presumptive α-secretase cleavage site of the amyloid-β (Aβ peptide led us to hypothesize that NIa could possess activity against Aβ. METHODOLOGY/PRINCIPAL FINDINGS: Western blotting results showed that oligomeric as well as monomeric forms of Aβ can be degraded by NIa in vitro. The specific cleavage of Aβ was further confirmed by mass spectrometry analysis. NIa was shown to exist predominantly in the cytoplasm as observed by immunofluorescence microscopy. The overexpression of NIa in B103 neuroblastoma cells resulted in a significant reduction in cell death caused by both intracellularly generated and exogenously added Aβ. Moreover, lentiviral-mediated expression of NIa in APP(sw/PS1 transgenic mice significantly reduced the levels of Aβ and plaques in the brain. CONCLUSIONS/SIGNIFICANCE: These results indicate that the degradation of Aβ in the cytoplasm could be a novel strategy to control the levels of Aβ, plaque formation, and the associated cell death.

  18. First intercomparison among laboratories involved in COST Action-TU1301 "NORM4Building": Determination of natural radionuclides in ceramics.

    Science.gov (United States)

    Xhixha, Gerti; Trinidad, José Antonio; Gascó, Catalina; Mantovani, Fabio

    2017-03-01

    This work describes the outcomes of the COST Action-TU1301 "NORM4Building" intercomparison on the determination of natural radioactivity in ceramics. Twenty-two laboratories involved in the intercomparison are evaluated for their performance using robust statistics. The reference values of (226)Ra ((214)Bi and (214)Pb) are determined to be 122 ± 11 Bq kg(-1) and 124 ± 14 Bq kg(-1), respectively and in secular equilibrium in the uranium chain while the reference values of (232)Th ((228)Ac) is determined to be 61 ± 6 Bq kg(-1) and that of (40)K was determined to be 955 ± 40 Bq kg(-1). Although the aim of the exercise was to determine the activity concentration of (226)Ra, (232)Th and (40)K and evaluation of the "Activity Concentration Index", laboratories were asked to report complete characterization of natural radionuclides. The results of this exercise pointed out a good performance among laboratories since the percentage of the acceptable results were above 90% for the radionuclides of interest. Based on these results, considering the systematic rejection of the results reported from a few laboratories we emphasize the need for quality control procedures. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Applications of GPR in archaeological prospecting and cultural heritage diagnostics: Research Perspectives in COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Benedetto, Andrea; Schettini, Giuseppe; Soldovieri, Francesco

    2013-04-01

    Ground Penetrating Radar (GPR) is a safe, non-destructive and non-invasive imaging technique that can be effectively used for advanced inspection of composite structures and for diagnostics affecting the whole life-cycle of civil engineering works. GPR can also be successfully employed in archaeological prospecting and cultural heritage diagnostics. In many Countries, where the archeological patrimony is an outstanding value (as Egypt, Israel, Greece, Central and South America), GPR is usually employed both as a diagnostic tool for the preventive detection of archeological structures and as the most advanced instrument able to prospect geometry and shape of underground valuable sites. However many uncertainties persist, because of several difficulties and ambiguities due to the complexity of the image processing in heterogeneous environment. It is possible to identify three main areas, in GPR field, that have to be addressed in order to promote the use of this technology in archaeological prospecting and cultural heritage diagnostics. These are: a) increase of the system sensitivity to enable the usability in a wider range of conditions, archeological sites are often located in impervious and critical environments; b) research novel data processing algorithms/analysis tools for the interpretation of GPR results; c) contribute to the development of new standards and guidelines and to training of end users, that will also help to increase the awareness of operators. It is also important to further investigate and promote a combined use of GPR with other non-invasive advanced techniques, typically used in the archeological investigation. In this framework, the COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar", proposed by a research team of "Roma Tre" University, Rome, Italy, has been approved in November 2012 and is going to start in April 2013. It is a 4-years ambitious project already involving 17 European Countries (AT, BE, CH, CZ, DE

  20. A ficha técnica de preparação como instrumento de qualidade na produção de refeições The technical cards as quality instrument for good manufacturing process

    Directory of Open Access Journals (Sweden)

    Rita de Cássia Akutsu

    2005-04-01

    Full Text Available As profundas transformações nos métodos e tecnologias de trabalho têm gerado mudanças no processo de produção de refeições. A ficha técnica de preparação foi objeto de uma análise, na perspectiva de discuti-la como instrumento de qualidade na produção de refeições. Foram utilizadas bases de dados, livros e formas eletrônicas de artigos para a pesquisa bibliográfica. A compreensão de que o nutricionista, na condição de profissional de saúde, possui como atribuição a atenção dietética, e de que esta deve estar associada à racionalização e à otimização do processo de produção de refeição, seguindo princípios diet��ticos, faz com que a Ficha Técnica de Preparo seja um instrumento de promoção à saúde. Sua construção como ferramenta de atenção dietética é, portanto, um dos desafios com vistas à necessária mudança de atitude dos profissionais, no que tange ao aprendizado e à aplicação de novas tecnologias, e ao fornecimento de alimento seguro.The methods and techniques major changes has stimulated the good manufacturing process studies. The manufacturing technical card has been analyzed as a meal manufacturing process quality instrument, and bibliographic studies, such as database, books and articles were the theoretical instruments. The nutritionist responsibility for the diet attention as a health professional member, attempting to the diet procedures to the meal manufacturing process makes, the Manufacturing Technical Card an instrument to the health promotion. Thus, the Manufacturing Technical Card can be a dietetic practice and management issue.

  1. Preparation and scaling up of a low phenylalanine enzymatic hydrolysate of bovine whey proteins Preparação e escalonamento de um hidrolisado enzimático de proteínas do soro de leite bovino

    Directory of Open Access Journals (Sweden)

    Marilisa Guimarães Lara

    2005-12-01

    Full Text Available We describe the preparation of pancreatic enzymes hydrolysate of milk whey proteins containing low levels of aromatic amino acids. Pancreatin and trypsin/chymotrypsin (6.3% w/w protein when used to hydrolyze whey proteins for 27 h at 37±2 ºC, released 74% of the Phe, 100% of the Tyr and 100% of the Trp as free amino acids. Most of the free aromatic amino acids present in 2 kg hydrolysate were separated from the remaining peptides and other amino acids by gel filtration on a 15 liter Sephadex G-25 column eluted with 5% acetic acid at 60 liters h-1 at 25ºC. The product, recovered in 37% yield, contained 0.70 mmol Phe, 0.41 mmol Tyr, and Foi descrita a preparação de um hidrolisado de proteínas do soro de leite bovino com enzimas pancreáticas, contendo baixos níveis de aminoácidos aromáticos. Quando utilizadas pancreatina e tripsina/quimotripsina, por 27h a 37±2ºC, foram liberados 74% de Phe, 100% de Tyr e 100% de Trp como aminoácidos livres. A maioria dos aminoácidos aromáticos livres, presentes em dois quilos de hidrolisado (15 litros, foi separada dos peptídeos e outros aminoácidos remanescentes por filtração em coluna de gel de Sephadex G-25C eluída com ácido acético 5%, fluxo de 60 litros por hora a 25ºC. O produto, recuperado com 37% de rendimento, continha 0,70 mmol de Phe, 0,41 mmol de Tyr e <0,01 mmol de Trp/100 mmol de aminoácidos recuperados. A composição em aminoácidos do hidrolisado foi similar às proteínas do soro com as quais foi preparado. Após adição de aminoácidos aromáticos apropriados, ele pode ser usado como fonte de nitrogênio para pacientes com fenilcetonuria ou tirosinemia.

  2. Consumo de óleo e gordura nas preparações do almoço self service
    Consumption of oil and fat from preparations of a self service lunch

    Directory of Open Access Journals (Sweden)

    M. M. A. AMORIM

    2010-11-01

    Full Text Available

    O consumo per capita de óleo e gordura das preparações de 22 cardápios do almoço self service de uma empresa foi avaliado. Cada ingrediente utilizado no seu preparo foi pesado antes e após a cocção, bem como as sobras não-consumidas após a distribuição. As últimas foram transformadas em gramas de seus respectivos ingredientes crus, sendo deduzidas das quantidades iniciais. Os pesos médios per capita dos ingredientes foram obtidos pela razão quantidade total consumida/número de refeições servidas. Foram calculadas as quilocalorias totais empregando os fatores de Atwater, com base numa tabela de composição de alimentos brasileira. As quantidades médias per capita consumidas no almoço foram: 1,5 mL e 2,3 mL de óleo e gordura, respectivamente, do feijão e arroz; 7,5 mL de óleo do prato protéico; 2,5 mL de óleo e margarina da guarnição; 6,9 g de maionese e 0,9 mL de azeite das saladas, totalizando 21,6 mL. Essa quantidade representou 14% do total energético da refeição (1171 kcal. O elevado teor no prato protéico (38,5% chamou a atenção, assim como, que as quantidades de óleo vegetal utilizadas no preparo de feijão e arroz não foram constantes, devendo ser padronizadas.

  3. A preparação de material terminológico em língua inglesa por meio de ferramentas linguístico-computacionais Preparation of terminological material in english by means of computational linguistic tools

    Directory of Open Access Journals (Sweden)

    Eduardo Batista da Silva

    2011-06-01

    Full Text Available O objetivo desse estudo é demonstrar, por meio de análise quantitativa e qualitativa, a eficácia de ferramentas linguístico-computacionais na seleção de terminologia para a produção de material terminológico. Serão apresentadas duas ferramentas linguístico-computacionais (WordSmith Tools e VocabProfile e, também, sugestões para que o ensino de termos ofereça resultados práticos. A fundamentação teórico-metodológica recorreu a Barros (2004; Berber Sardinha (2000; 2005; Biderman (2001; Cabré (2007; Cobb (2007; Nation, (2003 e Sinclair (2004. O corpus da pesquisa foi constituído exclusivamente de material escrito na língua inglesa em diversas áreas de especialidade. Os procedimentos de preparação de material terminológico são exemplificados a partir de uma das áreas de especialidades utilizadas nos corpora de pesquisa, as Redes Neurais Artificiais. Os resultados obtidos indicam que a utilização do Wordsmith Tools juntamente com o VocabProfile pode fornecer dados importantes para a pesquisa linguistica.This paper aims to demonstrate by means of quantitative and qualitative analyses the effectiveness of the linguistic computational tools in selecting terminology for the production of terminological material. Two linguistic computational tools will be introduced (WordSmith Tools e VocabProfile and also suggestions so as the teaching of terms may offer practical results. The theoretical-methodological approach relies on Barros (2004; Berber Sardinha (2000; 2005; Biderman (2001; Cabré (2007; Cobb (2007; Nation (2003 and Sinclair (2004. The research corpus was made solely of written material in English in several specialty languages. The procedures regarding terminological material preparation are exemplified with one of the specialty fields used in the research corpus, the Artificial Neural Networks. The obtained results indicate that the use of Wordsmith Tools in conjunction with VocabProfile might provide useful data for

  4. Development of Molecular Markers Linked to a Novel QTL for TuMV-C4 Resistance in Brassica rapa L. ssp. pekinensis%一个新的白菜苗期TuMV-C4抗性主效QTL定位及连锁分子标记开发

    Institute of Scientific and Technical Information of China (English)

    田希辉; 于拴仓; 苏同兵; 张凤兰; 余阳俊; 张德双; 赵岫云; 汪维红

    2014-01-01

    为寻找与TuMV抗性基因紧密连锁的分子标记,选用高抗病毒病大白菜自交系91-112和高感病毒病自交系T12-19以及由二者为双亲构建的包含100个株系的DH群体为材料,通过SSR和InDel标记的遗传分析,在A09上定位了一个新的与大白菜苗期TuMV-C4抗性相关的主效QTL位点BrTuA09。在此基础上,针对该QTL位点所在的标记区间,根据作图群体双亲的重测序结果,设计合成27对引物,其中11个InDel在双亲间具有多态性,且条带单一、扩增稳定;连锁分析发现,11个InDel标记均被定位在A09连锁群上BrTuA09的置信区间。利用BC1群体进行标记验证发现,这些标记对高抗单株选择的准确率均达到78%以上,可应用于分子标记辅助选择,为大白菜TuMV抗病分子育种奠定了良好的基础。%Our research constructed a molecular genetic map with a line 91-112 ( highly resistant to TuMV-C4),a highly susceptible line T12-19 and the DH population derived from microspore culture of F1(91-112 × T12-19). A total of 45 markers were mapped to the A09 chromosome,and a new QTL-BrTuA09 controlling TuMV-C4 re-sistance was identified. On this basis,27 pairs of primers were designed by screening the InDel loci on A09 chromo-some of Chinese cabbage based on genome re-sequencing. The results showed that distinct PCR amplification prod-ucts were obtained from the InDel markers. Among these markers,11 InDel were polymorphic on the tested parent materials,and mapped to the A09 chromosome. By linkage analysis,these markers were tightly linked to the QTL-BrtuA09 . The studies showed that these markers can be applied in marker-assisted selection in Chinese cabbage hy-brid breeding programs and improve the resistance to TuMV-C4 .

  5. Ação de diferentes preparações de extrato pirolenhoso sobre Brevipalpus phoenicis (GEIJSKES Action of different pyroligneous extract preparations when applied on Brevipalpus phoenicis (GEIJSKES

    Directory of Open Access Journals (Sweden)

    Mariangela Alves

    2007-08-01

    Full Text Available O objetivo deste trabalho foi avaliar a forma de ação de duas preparações de extrato pirolenhoso aplicadas diretamente sobre Brevipalpus phoenicis, que é o ácaro vetor da leprose dos citros, um dos principais problemas da citricultura Paulista. Para o experimento, foram utilizados ácaros adultos mantidos numa criação-estoque no laboratório de Acarologia da UNESP - Universidade Estadual Paulista, em Jaboticabal-SP. Os tratamentos foram constituídos por duas diferentes preparações (destilado e decantado de extrato pirolenhoso de eucalipto nas proporções EP:água de 1:600; 1:300 (normalmente recomendadas; 1:150; 1:75; 1:38; 1:19 e de água (testemunha, com 7 repetições. Cada parcela foi constituída de 10 ácaros mantidos sobre um fruto de laranja, em arena de 2,5 cm de diâmetro, delimitada com cola adesiva tipo Tanglefoot®. As aplicações foram efetuadas em Torre de Potter, pulverizando-se 2 mL por fruto das soluções correspondentes aos diferentes tratamentos. Os frutos foram mantidos em sala climatizada a 27±1ºC, e as avaliações foram realizadas 24 e 48 horas após a aplicação dos tratamentos, determinando-se o número de ácaros mortos (mortalidade e retidos na barreira adesiva (repelência. Os dois tipos de extrato pirolenhoso testados não apresentaram repelência significativa sobre Brevipalpus phoenicis; ambos induziram mortalidade significativa somente para concentrações acima de 1:150, com efeito mais pronunciado para o destilado; há um aumento na mortalidade de 24 para 48 horas após a aplicação; a ação protetora preconizada pela aplicação de baixas doses (1:300 a 1:600 nas plantas não é devida à mortalidade e repelência pelo contato direto do extrato pirolenhoso sobre os ácaros.The aim of this work was to evaluate the acaricide and repellent effects of two different pyroligneous extract preparations (PE applied directly on Brevipalpus phoenicis. This mite is the vector or citrus leprosies, which

  6. Biological Characteristics of CD133+CD44+ Cancer Stem Cells Sorting from Laryngeal Carcinoma Cell Line TU177%喉癌TU177细胞系中CD133+CD44+肿瘤干细胞分选及特性分析

    Institute of Scientific and Technical Information of China (English)

    杨俊岭; 高伟; 王珏; 付荣; 陈波; 李伟艳; 温树信; 王斌全

    2016-01-01

    Objective :Magnetic activated cell sorting was used to separate CD133+CD44+ cancer cells from laryngeal car-cinoma TU177 cell line. Analysis the biological characteristics of these subpopulations .Methods :TU177 cells were subjected to magnetic activated cell sorting to obtain CD133+CD44+、CD133+CD44-、CD133-CD44+、CD133-CD44-cells. Evaluate the efficiency of magnetic separation by flow cytometry . Test cell proliferation,migration,invasion,adhesion,colony forming ability of the cells.Results: CD133+CD44+ cells show higher proliferation,migration,invasion,adhesion,clone ability than other group(P<0.0001).Conclusions:TU177 cells can be serparated by Magnetic activated cell sorting effectively. CD133 is more powerful than CD44.Our study may provide evidence for target treatment of laryngeal cancer.%目的:免疫磁珠分选喉癌TU177细胞系中的CD133+CD44+细胞,探讨CD133+CD44+细胞作为肿瘤干细胞的生物学特性。方法:培养喉癌TU177细胞,采用免疫磁珠分选技术分选CD133+CD44+、CD133+CD44-、CD133-CD44+、CD133-CD44-细胞,流式检测分选效率,检测各组细胞的增殖、侵袭、迁移、粘附、克隆形成能力。结果:CD133+CD44+细胞的增殖、迁移、侵袭、粘附、克隆能力均明显高于其他组,差异有统计学意义(P<0.0001)。结论:免疫磁珠技术能有效进行TU177细胞系的分选,CD133+CD44+细胞亚群具有强增殖、侵袭、迁移、粘附、克隆形成能力,具有肿瘤干细胞特征,CD133作为干细胞标志物,其干细胞特性强于CD44,可为喉癌的进一步靶向治疗提供依据。

  7. Exchanging knowledge and working together in COST Action TU1208: Short-Term Scientific Missions on Ground Penetrating Radar

    Science.gov (United States)

    Santos Assuncao, Sonia; De Smedt, Philippe; Giannakis, Iraklis; Matera, Loredana; Pinel, Nicolas; Dimitriadis, Klisthenis; Giannopoulos, Antonios; Sala, Jacopo; Lambot, Sébastien; Trinks, Immo; Marciniak, Marian; Pajewski, Lara

    2015-04-01

    This work aims at presenting the scientific results stemming from six Short-Term Scientific Missions (STSMs) funded by the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (Action Chair: Lara Pajewski, STSM Manager: Marian Marciniak). STSMs are important means to develop linkages and scientific collaborations between participating institutions involved in a COST Action. Scientists have the possibility to go to an institution abroad, in order to undertake joint research and share techniques/equipment/infrastructures that may not be available in their own institution. STSMs are particularly intended for Early Stage Researchers (ESRs), i.e., young scientists who obtained their PhD since no more than 8 years when they started to be involved in the Action. Duration of a standard STSM can be from 5 to 90 days and the research activities carried out during this short stay shall specifically contribute to the achievement of the scientific objectives of the supporting COST Action. The first STSM was carried out by Lara Pajewski, visiting Antonis Giannopoulos at The University of Edinburgh (United Kingdom). The research activities focused on the electromagnetic modelling of Ground Penetrating Radar (GPR) responses to complex targets. A set of test scenarios was defined, to be used by research groups participating to Working Group 3 of COST Action TU1208, to test and compare different electromagnetic forward- and inverse-scattering methods; these scenarios were modelled by using the well-known finite-difference time-domain simulator GprMax. New Matlab procedures for the processing and visualization of GprMax output data were developed. During the second STSM, Iraklis Giannakis visited Lara Pajewski at Roma Tre University (Italy). The study was concerned with the numerical modelling of horn antennas for GPR. An air-coupled horn antenna was implemented in GprMax and tested in a realistically

  8. ¿Arqui tec tu ra para quién? ¿Arquitectura para qué?

    Directory of Open Access Journals (Sweden)

    Carlos Vera Guardia

    2004-01-01

    Full Text Available En este dis cur so el au tor pre sen ta una vi sión crítica, filosófica, ética y epistémica de la Arquitec - tura entendida como una disciplina cientí fica y hu - manís tica. En tal sentido Arquitectura es sinónimo de: i representación sim bólica, conceptual y estéti - ca de la rea li dad, ii de sa rro llo de las ba ses ma te ria - les de la vida en su con di ción de há bi tat, y iii com - pro mi so éti co con la de fen sa y pro tec ción de las in - teracciones bióticas y ecológicas entre la naturaleza y el pro gre so hu ma no. ¿Pa ra quién y para qué?, son dos “pre gun tas para pen sar”, acer ca de los fi nes y los me dios que con vier ten a la Arqui tec tu ra en un sa ber in ter pre tar y cons truir el há bi tat de la vida hu - mana desde la perspectiva histó rica, social, am - bien tal, eco nó mi ca y po lí ti ca en la que los se res hu - manos se desenvuelven.

  9. “MONTEVIDEO, TU CASA”: ELEMENTOS PARA UNA LECTURA DEL CUERPO EN EL ESPACIO PÚBLICO MODERNO.

    Directory of Open Access Journals (Sweden)

    Cecilia Seré Quintero

    2015-11-01

    Full Text Available El eslogan “Montevideo, tu casa” utilizado por la Intendencia Municipal de Montevideo (Uruguay entre 1991 y 2006, funciona como punto de partida para el análisis de las formas modernas que toma el espacio público urbano. La ciudad, escenario de la modernidad, da cuenta de la indistinción que se establecerá entre lo público y lo privado a partir de la emergencia de lo social, la consolidación del Estado-nación y la configuración de la economía política como parámetro de lectura de la realidad. Con ello, la política se verá reducida a la gestión y administración de las necesidades de la población, y la vida en términos biológicos tendrá allí un lugar central, factor clave para la organización de un espacio público que se presenta como una extensión del ámbito doméstico. El proceso de modernización de la ciudad de Montevideo es tomado en este artículo para dar cuenta de los efectos de una política contemporánea que se rige por la primacía de lo económico y donde parecen reducirse las distinciones entre el espacio público y el doméstico a cuestiones de tamaño o escala, teniendo como correlato una biologización de la vida pública y por tanto de la política.

  10. Ground-penetrating radar investigation of St. Leonard's Crypt under the Wawel Cathedral (Cracow, Poland) - COST Action TU1208

    Science.gov (United States)

    Benedetto, Andrea; Pajewski, Lara; Dimitriadis, Klisthenis; Avlonitou, Pepi; Konstantakis, Yannis; Musiela, Małgorzata; Mitka, Bartosz; Lambot, Sébastien; Żakowska, Lidia

    2016-04-01

    The Wawel ensemble, including the Royal Castle, the Wawel Cathedral and other monuments, is perched on top of the Wawel hill immediately south of the Cracow Old Town, and is by far the most important collection of buildings in Poland. St. Leonard's Crypt is located under the Wawel Cathedral of St Stanislaus BM and St Wenceslaus M. It was built in the years 1090-1117 and was the western crypt of the pre-existing Romanesque Wawel Cathedral, so-called Hermanowska. Pope John Paul II said his first Mass on the altar of St. Leonard's Crypt on November 2, 1946, one day after his priestly ordination. The interior of the crypt is divided by eight columns into three naves with vaulted ceiling and ended with one apse. The tomb of Bishop Maurus, who died in 1118, is in the middle of the crypt under the floor; an inscription "+ MAVRVS EPC MCXVIII +" indicates the burial place and was made in 1938 after the completion of archaeological works which resulted in the discovery of this tomb. Moreover, the crypt hosts the tombs of six Polish kings and heroes: Michał Korybut Wiśniowiecki (King of the Polish-Lithuanian Commonwealth), Jan III Sobieski (King of the Polish-Lithuanian Commonwealth and Commander at the Battle of Vienna), Maria Kazimiera (Queen of the Polish-Lithuanian Commonwealth and consort to Jan III Sobieski), Józef Poniatowski (Prince of Poland and Marshal of France), Tadeusz Kościuszko (Polish general, revolutionary and a Brigadier General in the American Revolutionary War) and Władysław Sikorski (Prime Minister of the Polish Government in Exile and Commander-in-Chief of the Polish Armed Forces). The adjacent six crypts and corridors host the tombs of the other Polish kings, from Sigismund the Old to Augustus II the Strong, their families and several Polish heroes. In May 2015, the COST (European COoperation in Science and Technology) Action TU1208 "Civil engineering applications of Ground Penetrating Radar" organised and offered a Training School (TS) on the

  11. Estudo preliminar do potencial cariogênico de preparações doces da merenda escolar através do pH da saliva Preliminary study of cariogenic potential of sweet school meal preparations through saliva pH

    Directory of Open Access Journals (Sweden)

    Cristiane Pinheiro Lázaro

    1999-12-01

    Full Text Available Objetivou-se determinar a curva de pH da saliva após o consumo de preparações doces, relacionar as variáveis teor de glicídios e pH, e discutir dados sobre o uso de preparações doces na merenda escolar do município de Niterói, RJ, com diferentes teores de glicídios e diferentes consistências, com crianças em idade pré-escolar e escolar, atendidas no Departamento de Odontologia Clínica da Faculdade de Odontologia da Universidade Federal Fluminense. Para os testes, foram selecionadas 52 crianças eutróficas, sadias, sem uso de medicamentos que alterassem o fluxo e a composição da saliva e com baixo e alto risco de cárie. Neste grupo, 54% apresentavam idade de 7 a 10 anos. Foi medido o pH da saliva, com fitas indicadoras, antes e após o consumo destas preparações, em um intervalo de 10, 20, 40 e 60 minutos. O tratamento estatístico foi realizado através do "software" Epi Info e foram realizados os testes de Qui-Quadrado e "t" de Student. Nenhuma das curvas de pH da saliva, para todas as preparações testadas e grupos de risco atingiram valor de pH considerado crítico (5,3 - 5,5. As curvas de pH médio da saliva, para todas as preparações testadas, oscilaram em valores de pH mais elevados para o grupo de baixo risco de cárie e em valores mais baixos para o grupo de alto risco de cárie. As curvas de pH médio da saliva do grupo de baixo risco demonstraram queda mais lenta e recuperação mais tardia, enquanto que no grupo de alto risco a queda e a recuperação do pH foram mais imediatas. A consistência das preparações foi preponderante sobre o teor de glicídios. Recomenda-se que o planejamento da merenda escolar leve em consideração o efeito da consistência das preparações doces servidas.The aim of this study was to establish the saliva pH curve in response to sweet foods, with different carbohydrate rates and different consistencies, frequently found in the school meal program in Niterói, Rio de Janeiro

  12. Tu-yuan-ce-fu and Compilation of Encyclopedia in the Tang Dynasty%《兔园策府》与唐代类书的编纂

    Institute of Scientific and Technical Information of China (English)

    王璐

    2015-01-01

    类书起源很早,是中国传统文化的产物。唐代修撰类书,始于建国之初,大抵是出于帝王之愿,试图把所有的知识进行分类便于提纲挈领地学习,因而唐代还有不少文人学士所编的类书就因循这样的法则。敦煌本《兔园策府》也是其中之一,它的白文本在唐宋间十分流行,《兔园策府》成书时间在《初学记》前,因而偶句之体例不是自徐坚始,很有可能始自《兔园策府》。%The encyclopedia originated early, is the product of Chinese traditional culture. The encyclopedias com-pilation started at the foundation of Tang Dynasty, which is probably out of the emperor willing to try to put all the knowledge classification facilitates learning focused on the vital. Then the encyclopedias compiled by scholars was procrastinating this rule as well in Tang Dynasty. Tu-yuan-ce-fu ( Encyclopedia Rabbit Garden) of Dunhuang edi-tion is one of them;its full form was very popular among the Tang and Song Dynasties. Tu-yuan-ce-fu was comple-ted before Chu-xue-ji ( The Primary Anthology) . Alternate style was not created by Xu Jian but probably from Tu-yuan-ce-fu.

  13. Tu-yuan-ce-fu and Compilation of Encyclopedia in the Tang Dynasty%《兔园策府》与唐代类书的编纂

    Institute of Scientific and Technical Information of China (English)

    王璐

    2014-01-01

    类书起源很早,是中国传统文化的产物。唐代修撰类书,始于建国之初,大抵是出于帝王之愿,试图把所有的知识进行分类便于提纲挈领地学习,因而唐代还有不少文人学士所编的类书就因循这样的法则。敦煌本《兔园策府》也是其中之一,它的白文本在唐宋间十分流行,《兔园策府》成书时间在《初学记》前,因而偶句之体例不是自徐坚始,很有可能始自《兔园策府》。%The encyclopedia originated early, is the product of Chinese traditional culture. The encyclopedias com-pilation started at the foundation of Tang Dynasty, which is probably out of the emperor willing to try to put all the knowledge classification facilitates learning focused on the vital. Then the encyclopedias compiled by scholars was procrastinating this rule as well in Tang Dynasty. Tu-yuan-ce-fu ( Encyclopedia Rabbit Garden) of Dunhuang edi-tion is one of them;its full form was very popular among the Tang and Song Dynasties. Tu-yuan-ce-fu was comple-ted before Chu-xue-ji ( The Primary Anthology) . Alternate style was not created by Xu Jian but probably from Tu-yuan-ce-fu.

  14. Transcriptional Profiling of Newly Generated Dentate Granule Cells Using TU Tagging Reveals Pattern Shifts in Gene Expression during Circuit Integration1,2

    Science.gov (United States)

    Chatzi, Christina; Shen, Rongkun; Goodman, Richard H.

    2016-01-01

    Abstract Despite representing only a small fraction of hippocampal granule cells, adult-generated newborn granule cells have been implicated in learning and memory (Aimone et al., 2011). Newborn granule cells undergo functional maturation and circuit integration over a period of weeks. However, it is difficult to assess the accompanying gene expression profiles in vivo with high spatial and temporal resolution using traditional methods. Here we used a novel method [“thiouracil (TU) tagging”] to map the profiles of nascent mRNAs in mouse immature newborn granule cells compared with mature granule cells. We targeted a nonmammalian uracil salvage enzyme, uracil phosphoribosyltransferase, to newborn neurons and mature granule cells using retroviral and lentiviral constructs, respectively. Subsequent injection of 4-TU tagged nascent RNAs for analysis by RNA sequencing. Several hundred genes were significantly enhanced in the retroviral dataset compared with the lentiviral dataset. We compared a selection of the enriched genes with steady-state levels of mRNAs using quantitative PCR. Ontology analysis revealed distinct patterns of nascent mRNA expression, with newly generated immature neurons showing enhanced expression for genes involved in synaptic function, and neural differentiation and development, as well as genes not previously associated with granule cell maturation. Surprisingly, the nascent mRNAs enriched in mature cells were related to energy homeostasis and metabolism, presumably indicative of the increased energy demands of synaptic transmission and their complex dendritic architecture. The high spatial and temporal resolution of our modified TU-tagging method provides a foundation for comparison with steady-state RNA analyses by traditional transcriptomic approaches in defining the functional roles of newborn neurons. PMID:27011954

  15. 菌株Tu 49 代谢产物的分离及抗菌素活性的初步研究%The Isolation of the Strain Tu49 and the Study on the Activity of Its Antibiosis

    Institute of Scientific and Technical Information of China (English)

    迪力夏提·托呼提; 阿不都克里木·热依木

    2001-01-01

    从吐鲁番葡萄沟、苏公塔、五道林、交角沟等郊区采集的52种土样中筛选到一株能产有抗菌素活性的好气菌Tu49,革兰氏阳性,类群为黄色类群。所产生的抗菌素,对不同的微生物有明显的抑制作用。最适生长温度为30℃~32℃,最适pH为6.8~7.5,气生菌丝和孢子丝为无色,基丝体为黄色,产黄色可溶性色素。%We isolated aerial strain Tu 49 from 52 samples of soil in 5 outskirts of Turpan city, its colony was golden and Gram positives(G+).The antibiosis from strain Tu49 inhibited different microorganisms, The optimum growth temperature was 30~32℃ and pH was 6.8~7.5;aerial hypha and spore hypha was colourless, my cellium was yellow. Which can produce soluble figment.

  16. [Classes of crude drugs and its distribution of producing area in the attached illustrations in Ben cao tu jing (Illustrated Classic of Materia Medica)].

    Science.gov (United States)

    Xu, T; Peng, H S

    2016-03-01

    Ben cao tu jing (Illustrated Classic of Materia Medica) is the earliest extant atlas book of materia medica in China, with 933 attached drawings. Among them, the largest portion, amounting to 670, are herbaceous plants, mostly commonly used, with definite marks of the origin producing areas, distributed across 149 administrative divisions(prefectures and counties) of the Song Dynasty, most of them in Northern area which were distributed denser than those in Southern area. The densest ones were located in Southern Shanxi, Eastern Sichuan and Eastern Anhui. In the attached drawings, the frequency of highest occurrence appeared in this Classic are three prefectures, Chuzhou, Shizhou and Guangzhou.

  17. The Comparative Study between“Ni/Nin”in Chinese and“Tu/Vous”in French Based on“Power and Solidarity”

    Institute of Scientific and Technical Information of China (English)

    杨悦

    2012-01-01

      The selection of the second person pronouns embodies the power and solidarity relations in social communication. The paper studies“Ni/Nin”in Chinese and“Tu/Vous”in French from sociolinguistic, cultural and pragmatic perspectives by basing on“Power and Solidarity”. Through the comparative study of this two pairs, not only can we observe the historical process of personal pronouns, and analyze the similarities and dissimilarities of the two languages which hide behind Chinese and French cultural backgrounds, but also may further understand their pragmatic principles in various context.

  18. Melhor o cozinheiro? Um percurso sobre a dimensão de gênero da preparação da comida (Europa ocidental, séculos XVI-XIX A male cook, is it better? An overview on the gender dimension of cooking ( Western Europe, 16th to 19th centuries

    Directory of Open Access Journals (Sweden)

    Raffaella Sarti

    2012-12-01

    Full Text Available Este artigo analisa a preparação da comida na Europa ocidental (séculos XVI a XIX, enfocando sua dimensão de gênero. São consideradas três principais variáveis: estratificação social, geografia e tempo. Sugere que na Itália, Espanha e França, no início do período moderno, os cozinheiros empregados nas cortes e pela aristocracia eram geralmente homens; a feminização da preparação da comida começou na França a partir do século XVIII. Na Europa central e do norte, as mulheres das classes mais altas estavam muito mais envolvidas na preparação da comida, embora a moda da cozinha francesa nos séculos XVII e XVIII tenha implicado no recurso crescente de homens cozinheiros. O artigo sugere explicações para essas diferenças e tendências através dos tempos, e discute o papel da nutrição e cozinha na definição da identidade feminina em contextos diferentes.This paper analyses the preparation of food in Western Europe (16th-19th centuries, focusing on its gendered dimension. Three main variables are considered: social stratification, geography and time. It suggests that in Italy, Spain and France in early modern times the cooks employed at the courts and by the aristocracy were generally men; a feminization of the preparation of food started in France from the 18th century onwards. In Central and Northern Europe women were much more involved in the preparation of food in the upper classes, too, even though the fashion for French cuisine in the 17th and 18th centuries implied a growing recourse to male cooks. The paper suggests explanations of these differences and trends over time and discusses the role of nurturing and cooking for the definition of the female identity in different contexts.

  19. Evaluation and additional recommendations for preparing a whole blood control material Avaliação e recomendações adicionais para a preparação de material sangüíneo integral para controles

    Directory of Open Access Journals (Sweden)

    Nilda E. Fink

    1998-04-01

    Full Text Available OBJECTIVE: The assessment of an easy to prepare and low cost control material for Hematology, available for manual and automated methods. MATERIAL AND METHOD: Aliquots of stabilized whole blood were prepared by partial fixation with aldehydes; the stability at different temperatures (4. 20 and 37 °C during periods of up to 8-9 weeks and aliquot variability with both methods were controlled. RESULTS: Aliquot variability with automated methods at day 1, expressed as CV% (coefficient of variation was: white blood cells (WBC 2.7, red blood cells (RBC 0.7, hemoglobin (Hb 0.6, hematocrit (Hct 0.7, mean cell volume (MCV 0.3, mean cell hemoglobin (MCH 0.6, mean cell hemoglobin concentration (MCHC 0.7, and platelets (PLT 4.6. The CV (coefficient of variation percentages obtained with manual methods in one of the batches were: WBC 23, Hct 2.8, Hb 4.5, MCHC 5.9, PLT 41. Samples stored at 4ºC and 20ºC showed good stability, only a very low initial hemolysis being observed, whereas those stored at 37ºC deteriobed a rapidly (metahemoglobin formation, aggregation of WBC and platelets, as well as alteration of erythrocyte indexes. CONCLUSIONS: It was confirmed that, as long as there is no exposure to high temperatures during distribution, this material is stable, allowing assessment, both esternal and internal, for control purposes, with acceptable reproductivity, both for manual and auttomatic methods.OBJETIVO: Avaliar material de controle para hematologia, de fácil preparação e baixo custo, que poderá ser usado por métodos manuais e automatizados. MATERIAL E MÉTODO: Alíquotas de sangue estabilizado foram preparadas por fixação parcial com aldeídos. Foram estudadas a estabilidade a diferentes temperaturas (4, 20, 37ºC durante períodos de até 8-9 semanas e a variabilidade das alíquotas para ambos os métodos. RESULTADOS: A variabilidade entre alíquotas, expressada em CV% (coeficiente de variação com métodos automatizados no primero dia

  20. Preparação e caracterização de organobentonita modificada com ionenos alifáticos Preparation and characterization of aliphatic ionene-modified organobentonite

    Directory of Open Access Journals (Sweden)

    Arão P. Costa Filho

    2005-07-01

    Full Text Available A preparação de complexos bentonita-ionenos a partir da adsorção de tetrametil-[x,y]-ionenos, onde x= 2 e y= 4,6,10 ou 12, e o 2-hidróxi-3-cloro-ioneno, EPI-DMA, foi realizada com a finalidade de observar o comportamento do tamanho do espaçador do ioneno na expansão basal (d001 das camadas de uma bentonita comercial do Brasil. Os complexos foram caracterizados por espectrometria de absorção na região do infravermelho, termogravimetria, fluorescência e difração de raios X. A partir das análises por fluorescência e difração de raios X dos complexos, foi observado que os poli-quaternários de amônio são adsorvidos nas superfícies internas e externas da argila delaminada, acima da capacidade de troca catiônica, CTC = 91 meq/100g de argila (calculada a partir de dados de fluorescência de raios X. A expansão basal das camadas da argila com os diferentes policátions revelou que o tamanho do espaçador é o fator mais importante, independentemente das massas molares dos ionenos, tal como foi observado principalmente entre os complexos Bt-2,10- e Bt-2,12-ioneno, com massas molares de 12000 e 5000 g/mol, respectivamente.The preparation of bentonite-ionene complexes based on the adsorption of 2y-ionenes, where y = 4, 6, 10 or 12 and an ionene of the epichloridrin-dimethylamine EPI-DMA kind, was carried out in order to observe the behavior of the ionene spacer size in the expansion of the basal space (d-spacing, d001 of a Brazilian commercial bentonite. The complexes were characterized by adsorption spectromety in the infrared region, thermogravimetry, fluorescence and X-Ray diffraction. Ionenes were synthesized and characterized by infrared and nuclear magnetic resonance, the latter technique being useful for determining the average number molecular weight values. Based on the clay-ionenes complexes it could be observed that the ammonium polyquaternaries are adsorbed on the inner and outer clay surfaces, above the ion exchange

  1. Use of sawdust Eucalyptus sp. in the preparation of activated carbons Utilização de serragem de Eucalyptus sp. na preparação de carvões ativados

    Directory of Open Access Journals (Sweden)

    Gabriela Martucci Couto

    2012-02-01

    Full Text Available Wood sawdust is a solid residue, generated in the timber industry, which is of no profitable use and can cause serious environmental problems if disposed inadequately. The aim of this study was to use the eucalyptus sawdust in the preparation of activated carbons AC and test them as adsorbents of methylene blue (MB and phenol, representative pollutants from aqueous effluents of various industries. The eucalyptus sawdust was characterized by instrumental analysis such as elementary analysis (CHNS-O, thermogravimetric analysis (TGA, infrared spectroscopy (FTIR and scanning electron microscopy (SEM. The activated carbons were prepared by physical activation with carbon dioxide AC_CO2, (10º C min-1, 850º C, 1h and by chemical activation with potassium carbonate AC_K2CO3 (10º C min-1, 850º C, 3h. The AC_CO2 and AC_K2CO3 were characterized by CHN-O, TGA, FTIR, N2 adsorption/desorption (BET to evaluate the specific surface area and SEM. The resulting activated carbons were tested for their ability to adsorb MB and phenol in water. The activated carbons produced in this work were predominantly microporous and showed specific surface area of about 535 m² g-1. The AC_K2CO3 was more effective in the adsorption of MB (81 mg g-1 and phenol (330 mg g-1 than AC_CO2 (32 mg g-1 and 172 mg g-1, respectively, for MB and phenol.A serragem é um resíduo sólido, gerado na indústria madeireira, que não tem uso rentável e pode causar sérios problemas ambientais quando disposta inadequadamente. Neste estudo, objetivou-se utilizar a serragem de eucalipto na preparação de carvões ativados (AC e testá-los como adsorventes do corante azul de metileno (MB e fenol; moléculas que representam poluentes de efluentes industriais. A serragem de eucalipto foi caracterizada por análises instrumentais, tais como: análise elementar (CHNS-O, análise termogravimétrica (TGA, espectroscopia na região do infravermelho (FTIR e microscopia eletrônica de varredura (SEM

  2. Efeito de preparações de produtos naturais (cravo da índia e tomate) e de um produto sintético na marcação de constituintes sangüineos com tecnécio-99m e na morfologia de hemácias isoladas de sangue de ratos wistar

    OpenAIRE

    Paoli,Severo de

    2007-01-01

    Existe um grande interesse na avaliação dos efeitos biológicos de produtos naturais e sintéticos. Constituintes sangüíneos marcados com tecnécio-99m (99mTc) são usados em medicina nuclear. O objetivo desse trabalho foi estudar os efeitos do Cravo da índia (Caryophyllus aromaticus L.) e do Óxido de Zinco Eugenol (OZE) (preparação usada em Ciências da Saúde) na marcação de constituintes sangüíneos com 99mTc e na morfologia de células vermelhas do sangue (CVS) e a ação de um extrato de tomate...

  3. Como melhorar a preparação física dos alunos do 3º ano vocacional da Academia de Música de Vilar do Paraíso no âmbito da disciplina de Técnica de Dança Clássica

    OpenAIRE

    Pinto, Joana Queirós Moreira Espanha

    2013-01-01

    Relatório Final de Estágio apresentado à Escola Superior de Dança, com vista à obtenção do grau de Mestre em Ensino de Dança. O treino físico em dança, para além do desenvolvimento técnico-académico, constitui-se como um pilar fundamental no contexto da prática da dança, quer para estudantes quer para profissionais. Um bailarino completo e altamente competente no desempenho das suas funções será assim o resultado de um conjunto de fatores que abarcam uma eficaz preparação física aliada a u...

  4. Validação de método para avaliação da qualidade sanitária de preparação cosmética de base lipófila Validation of method to evaluate sanitary quality of lipophilic base cosmetics

    OpenAIRE

    Nádia Araci Bou-Chacra; Mitsuko Taba Ohara

    2003-01-01

    A validação de método para a avaliação da qualidade sanitária de produto cosmético de base lipófila foi efetuada empregando-se neutralizantes químicos e diluição para a eliminação de efeito inibitório no crescimento microbiano. Para a preparação da amostra foi empregado miristato de isopropila e polissorbato 20. Além dessas substâncias, foi adicionado ao diluente 0,5% (p/v) de lecitina de soja visando à neutralização do cloreto de benzalcônio. Os ensaios foram efetuados objetivando a comprova...

  5. Misturas de acetato de celulose-brometo de poli{[9,9-bis(6'-N,N,N-trimetilamónio) hexil}fluoreno-fenileno}: preparação, caracterização e cinética de libertação

    OpenAIRE

    Gomes, João Nuno Pinto Lopes

    2010-01-01

    Os polieletrólitos conjugados são materiais importantes com aplicações em áreas como os sensores químico / biológicos, apresentando ainda grande potencial em áreas que incluem sistemas de energia fotovoltaica e diodos emissores de luz (LEDs). Estes polímeros iónicos conjugados também são relevantes para aplicações de auto agregação e para a preparação de filmes utilizando metodologias baseadas no solvente, tais como impressões a jacto de tinta e tela. Os polímeros baseado...

  6. Preparação, modoficação, caracterização e aplicação de catalisadores visando a degradação de compostos orgânicos poluentes por processos oxidativos avançados

    OpenAIRE

    Thiago Lewis Reis Hewer

    2010-01-01

    Neste trabalho focou-se na preparação de materiais com aplicação em processos de tratamento de compostos orgânicos poluentes. Especificamente, buscou-se correlacionar as propriedades físicas e químicas dos materiais com as suas respostas catalíticas nos processos de fotocatálise heterogênea e de ozonização. Empregando-se o método de precipitação, preparou-se óxidos mistos de TiO2 e CeO2 em diferentes proporções. A caracterização destes materiais mostrou uma correlação entre a formação de part...

  7. Estudos da preparação de superfície da liga AA6063T5 e do desenvolvimento de revestimentos híbridos orgânico-inorgânicos a base de siloxano-PMMA para aplicação anticorrosiva

    OpenAIRE

    Teixeira, Dilton Gonçalves [UNESP

    2010-01-01

    Este trabalho está basicamente dividido em duas partes. A primeira está relacionada à limpeza da superfície da liga de alumínio AA6063T5 e sua influência na proteção contra a corrosão. A segunda parte se refere à preparação de revestimentos híbridos orgânicoinorgânico pelo processo sol-gel e deposição sobre a superfície da liga de alumínio por dipcoating. Três amostras de liga AA6063T5 foram investigadas: como recebida, debastada e polida mecanicamente e tratada quimicamente. O tratamento mec...

  8. Identification of probiotics strain Tu-569 from rabbit cecum and property analysis of its extracellular antibacterial product%兔源益生菌Tu-569菌株鉴定与其胞外产抑菌物性质分析

    Institute of Scientific and Technical Information of China (English)

    柴玉龙; 尚伟; 姜军坡; 王世英; 朱宝成

    2012-01-01

    目的 对兔源益生菌Tu-569菌株进行菌落形态和菌体形态观察、生理生化特性测定及16S rDNA序列系统发育分析以确定其种属.方法 以大肠埃希菌为指示菌,通过琼脂打孔扩散法分析Tu-569菌株胞外产抑菌物性质,初步评价该抗菌物活性.结果 Tu-569菌株为Bacillus velezensis; Tu-569菌株胞外产抑菌物为分子量3.5~8.0kD的多肽,可被饱和度60%的硫酸铵盐析;该抗菌肽热稳定性较好,但可被蛋白酶降解;采用正丁醇萃取该抗菌肽不会降低其活性;其活性可被Mn2+离子增强,但在强酸(pH 3)强碱(pH11)环境中会大幅度降低;当存在表面活性剂和β-巯基乙醇时,抗菌肽抑菌活性完全丧失.每1mg粗蛋白中含有的抗菌肽抑制大肠埃希菌的能力相当于2μg硫酸庆大霉素、10μg硫酸链霉素或50μg土霉素.结论 该抗菌肽性质稳定,具有较高的抗菌活性,在畜牧业、养殖业、食品等领域中有一定的应用价值.%Objective Works were done to identify probiotic strain Tu-569 and to analyze properties of its extracellular antibacterial product. Methods Identification was carried out by observations for colony morphology and cell shape, determinations of physiological, biochemical characteristics and sequence analysis. Properties analysis for extracellular antibacterial product were done with various treatments, such as salting out with ammonium sulfate, extraction with different organic solvent, heating, different pH environment, proteases, ions, protein protective and denaturant agents. The activity of antibacterial product was evaluated by agar diffusion assay with Escherichia coli as indicator bacteria. Results Strain Tu-569 was identified as Bacillus velezensis. The extracellular antibacterial product was polypeptide with molecular weight between 3.5kD to 8.0kD. The majority antibacterial polypeptide was salted out by 60% ammonium sulfate. The antibacterial polypeptide showed a good stability

  9. PID参数继电反馈整定法的改进%Improvement of relay feedback tuing method of PID parameters

    Institute of Scientific and Technical Information of China (English)

    高峰

    2012-01-01

    In this paper,the relay feedback tuing method of PID parameters is improved.An integrator is set between the relay and controlled object. High frequency signal is weaken, the proportion of the fundamental wave in the harmonic wave is increased and the accuracy of describing function is improved.On this occasion,new tuing equations is presented. Practical emulation proves that the tuning method after improvement can get better performance than Astrom method and PM method.%提出了一种改进的继电整定法,在继电环节和被控对象之间设置一个积分器。促进了高频信号的衰减。使谐波中的基波成分所占比例得到提高,改善了描述函数的精度,并在此情况下推导出了新的整定公式。通过仿真证明。利用改进后的方法进行PID参数整定比传统的Astrom法和PM法性能更加优越。

  10. Raúl Gonzalez Tuñón desencuadernado. Políticas de la literatura, entre el libro y las publicaciones periódicas

    Directory of Open Access Journals (Sweden)

    Geraldine Rogers

    2015-11-01

    Full Text Available RESUMEN: el artículo revisa algunos supuestos sobre la producción literaria de Raúl González Tuñón en las décadas de 1920 y 1930 a partir de dos desplazamientos. En primer lugar, explora las políticas de la literatura que permitirían pensar, de manera menos esquemática que con la habitual categoría de “literatura política” con que se suelen abordar sus textos de la década del treinta, las apuestas y limitaciones de una escritura que desde su inicio se propuso como transformadora. En segundo lugar, el artículo pone el foco en la primera circulación de los textos, muchas veces en diarios y revistas, contextos formativos de una literatura que en gran medida surgió en sus páginas. Desencuadernar a Tuñón implica atender a la materialidad de sus escritos y explorar más allá de la tradicional figura del autor de libros, para indagar políticas de la escritura que articularon campos y prácticas, y experimentaron no sólo con nuevas formas estéticas sino también con diversos modos de circulación pública de las letras.

  11. 土族民间信仰的民族心理学解析%Tu Folk Beliefs of the National Psychology Analytical

    Institute of Scientific and Technical Information of China (English)

    王默

    2011-01-01

    Tu folk circulates with many unique folk beliefs,the folk beliefs are minority is an important part of traditional culture.Including fertility belief,reproductive worship,home most widely and typical worship god.This paper tries to ethnic psychology,reading from the perspective of representative of folk beliefs of tu folk beliefs,to explore the psychological factors produced.%土族民间存在着很多独具特色的民间信仰,这些民间信仰是土族传统文化的重要组成部分。其中丰产信仰、生殖崇拜、家神崇拜最为广泛和典型。本文试从民族心理学的角度入手,解读其中具有代表性的民间信仰,以探讨土族民间信仰产生的心理因素。

  12. GTPase domains of ras p21 oncogene protein and elongation factor Tu: analysis of three-dimensional structures, sequence families, and functional sites.

    Science.gov (United States)

    Valencia, A; Kjeldgaard, M; Pai, E F; Sander, C

    1991-06-15

    GTPase domains are functional and structural units employed as molecular switches in a variety of important cellular functions, such as growth control, protein biosynthesis, and membrane traffic. Amino acid sequences of more than 100 members of different subfamilies are known, but crystal structures of only mammalian ras p21 and bacterial elongation factor Tu have been determined. After optimal superposition of these remarkably similar structures, careful multiple sequence alignment, and calculation of residue-residue interactions, we analyzed the two subfamilies in terms of structural conservation, sequence conservation, and residue contact strength. There are three main results. (i) A structure-based alignment of p21 and elongation factor Tu. (ii) The definition of a common conserved structural core that may be useful as the basis of model building by homology of the three-dimensional structure of any GTPase domain. (iii) Identification of sequence regions, other than the effector loop and the nucleotide binding site, that may be involved in the functional cycle: they are loop L4, known to change conformation after GTP hydrolysis; helix alpha 2, especially Arg-73 and Met-67 in ras p21; loops L8 and L10, including ras p21 Arg-123, Lys-147, and Leu-120; and residues located spatially near the N and C termini. These regions are candidate sites for interaction either with the GTP/GDP exchange factor, with a GTPase-affected function, or with a molecule delivered to a destination site with the aid of the GTPase domain.

  13. A perspectiva ecológica: referências para a preparação e a cessação da estadia em acolhimento familiar de crianças Ecological perspective: guidelines for planning child's foster care placement

    Directory of Open Access Journals (Sweden)

    Paulo Delgado

    2012-01-01

    Full Text Available A colocação em Acolhimento Familiar e a cessação da estadia constituem transições ecológicas, pelas alterações que acarretam e pelos papéis que atribuem aos seus protagonistas. A preparação destas transições visa não eliminar os obstáculos, uma utopia que o grau de mudança não permite, mas diminui-los, para um nível que facilite o desenvolvimento da criança e a integração na fase seguinte da sua vida. Este artigo revê o trabalho de alguns autores que, no Reino Unido, se têm debruçado sobre a preparação da colocação e da cessação do Acolhimento Familiar. O modelo ecológico é utilizado como quadro teórico orientador daquela medida de protecção e identifica um conjunto de factores que potencializam o sucesso das transições.The placement in foster care and its ceasing are ecological transitions due to the changes they bring and because of the roles assigned to their protagonists. The preparation of such transitions aims to not diminish the obstacles, a utopia that the degree of change does not allow, but to diminish them to a degree that makes the child integration in his/her next phase of life easier. This article reviews the work of some British authors who have taken a special interest in the preparation and closure of children's foster care. It also intends to legitimize, from a theoretical point of view, the relevance of using the ecological model as a guiding framework of that protective measure and suggests a set of practices which enhance the success of the transitions.

  14. Efeito das condições de preparação na condutividade elétrica de blendas de poli(fluoreto de vinilideno e poli(o-metoxianilina Effect of preparation conditions on the electrical conductivity of blends of poly (vinylidene fluoride and poly(o-methoxyaniline

    Directory of Open Access Journals (Sweden)

    Luiz Francisco Malmonge

    1998-01-01

    Full Text Available Neste trabalho estudou-se o efeito das condições de preparação na condutividade elétrica de blendas condutoras de poli(fluoreto de vinilideno (PVDF com poli(o-metoxianilina (POMA. A condutividade elétrica e a qualidade dos filmes produzidos dependem significativamente do solvente, dopante e das condições de preparação das blendas. Filmes flexíveis e auto-sustentáveis da blenda PVDF/POMA dopada com o ácido p-tolueno sulfônico (TSA foram obtidos em condições otimizadas. Estes apresentam condutividade elétrica em uma larga faixa (10-12 - 10-2 S/cm, onde para baixos teores de POMA-TSA (In this work, the effect of preparation conditions on the properties of conductive blends of poly (vinylidene fluoride (PVDF and poly(o-methoxyaniline (POMA was studied. The quality and conductivity of the films produced depend significantly on the solvent, dopant and preparation conditions of the blends. Flexible, free-standing films of PVDF/POMA blends doped with p-toluene sulfonic acid (TSA were obtained in optimized conditions. These blends can exhibit electrical conductivity in a wide range (10-12 - 10-2 S/cm. Furthermore, relatively high conductivity values (10-7 S/cm are reached starting from low contents of POMA-TSA (<2,0% weight without definition of a percolation threshold.

  15. Electromagnetic modelling, inversion and data-processing techniques for GPR: ongoing activities in Working Group 3 of COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Giannopoulos, Antonis; van der Kruk, Jan

    2015-04-01

    This work aims at presenting the ongoing research activities carried out in Working Group 3 (WG3) 'EM methods for near-field scattering problems by buried structures; data processing techniques' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (www.GPRadar.eu). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. WG3 is structured in four Projects. Project 3.1 deals with 'Electromagnetic modelling for GPR applications.' Project 3.2 is concerned with 'Inversion and imaging techniques for GPR applications.' The topic of Project 3.3 is the 'Development of intrinsic models for describing near-field antenna effects, including antenna-medium coupling, for improved radar data processing using full-wave inversion.' Project 3.4 focuses on 'Advanced GPR data-processing algorithms.' Electromagnetic modeling tools that are being developed and improved include the Finite-Difference Time-Domain (FDTD) technique and the spectral domain Cylindrical-Wave Approach (CWA). One of the well-known freeware and versatile FDTD simulators is GprMax that enables an improved realistic representation of the soil/material hosting the sought structures and of the GPR antennas. Here, input/output tools are being developed to ease the definition of scenarios and the visualisation of numerical results. The CWA expresses the field scattered by subsurface two-dimensional targets with arbitrary cross-section as a sum of cylindrical waves. In this way, the interaction is taken into account of multiple scattered fields within the medium hosting the sought targets. Recently, the method has been extended to deal with through-the-wall scenarios. One of the

  16. Solid Phase Extraction of Bryophyte Lipids / Briofītu lipīdu cietfāzes ekstrakcijas frakcionēšana

    Directory of Open Access Journals (Sweden)

    Klavina Laura

    2015-11-01

    Full Text Available Pētījuma ietvaros izstrādāta vienkārša, ātra un viegli atkārtojama analītiska metode briofītu lipīdu frakcionēšanai. Lipīdu frakcijas frakcionēšanai izmantoja cietfāzes ekstrakciju ar silikagēlu un ar NH2 grupām modificētu silikagēlu, iegūtās vielu grupas kvantificēja kā to trimetilsilil atvasinājumus, izmantojot gāzes hromatogrāfiju-masas spektrometriju. Frakcionēšanu veica, izmantojot eluentu polaritātes gradienta principu (eluenti: heksāns, heksāns/hloroforms 5:1, hloroforms, kā pēdējo eluentu ar NH2 grupām modificēta silikagēla gadījumā izmantojot skābes (etiķskābes šķīdumu. Izpētes objekti bija divi Latvijā plaši izplatīti sūnaugi (Polytrichum commune, Dicranum polysetum, kuru sastāvā identificēja vairāk nekā 45 dažādus savienojumus, daudzi no kuriem ir ar augstu bioloģisko aktivitāti. Sūnaugu sastāvā identificēja sterolus, tokoferolus, taukskābes un citi savienojumus. Izmantotā frakcionēšanas shēma pavēra iespējas identificēt daudzus savienojumus, kuri kopējā ekstrakta hromatogrammā nebija uzrādīti, piemēram, alkānu rindas savienojumi, vairāki steroli. Līdz ar to sūnaugu lipīdu cietfāzes frakcionēšanas metode ļāva noteikt vielu klātbūtni, kuras atrodas mikrodaudzumos, bet kuras varēja ļaut izsekot brīofītu pārvērtībām bioģeoķīmiskos procesos. Ieteiktā frakcionēšanas metode ļāva arī identificēt vairākas vielu grupas, kuras varētu būt perspektīvas kā jaunas bioloģiski aktīvas vielas, kā arī palīdzēja labāk izprast briofītu metabolismu, piemēram starpsugu atšķirības un sastāva sezonālās mainības raksturu.

  17. Construye tu Música

    Directory of Open Access Journals (Sweden)

    Contreras, Antonio de

    2000-05-01

    Full Text Available En el Sistema Educativo actual la composición musical está contemplada en el tramo final de los estudios de Conservatorio, tras muchos años de adiestramiento técnico-instrumental y teórico. Por otra parte, si visitamos un Aula de Educación Plástica de un Instituto, podemos ver a los alumnos de Secundaria expresándose, canalizando su creatividad a través de colores, formas y volúmenes, sin prejuicios, con un mínimo y, en la mayoría de los casos, muy simple bagaje de conocimientos técnicos previos. Está claro que se trata de dos artes completamente diferentes, también es bien sabido y repetido hasta la saciedad el carácter abstracto de la música. Pero así y todo ¿Será posible arbitrar un modo de acercarse a la música desde dentro, desde la práctica de la composición musical de una forma directa y simple, sin necesidad de una formación musical de nivel superior, de forma parecida a como se plantea la actividad creativa en la educación plástica? ¿Será posible complementar dicha práctica con la subsiguiente interpretación de las composiciones y la crítica de las producciones ajenas del mismo nivel? Las preguntas no son, por supuesto, nuevas: ya los grandes pedagogos musicales del primer cuarto de siglo las plantearon y resolvieron cada uno a su modo. La siguiente comunicación pretende dar a conocer los resultados de una investigación en el Aula de música, realizada por el autor durante los cuatro últimos años, que parte de una propuesta original en este sentido.

  18. TuGen

    DEFF Research Database (Denmark)

    Gilling, Lasse

    An implementation of Mann's method of generating synthetic turbulence is described. A brief introduction to the method is given prior to some details on the implementation. Several tests to verify the implementation follows and finally the use of the program is described....

  19. Expression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA of Lactobacillus plantarum 423, monitored with real-time PCR.

    Science.gov (United States)

    Ramiah, K; van Reenen, C A; Dicks, L M T

    2007-05-30

    Expression of the mucus adhesion genes Mub and MapA, adhesion-like factor EF-Tu and bacteriocin gene plaA by Lactobacillus plantarum 423, grown in the presence of bile, pancreatin and at low pH, was studied by real-time PCR. Mub, MapA and EF-Tu were up-regulated in the presence of mucus, proportional to increasing concentrations. Expression of MapA was up-regulated in the presence of 3.0 g/l bile and 3.0 g/l pancreatin at pH 6.5. Similar results were recorded in the presence of 10.0 g/l bile and 10.0 g/l pancreatin at pH 6.5. Expression of Mub was down-regulated in the presence of bile and pancreatin, whilst the expression of EF-Tu and plaA remained unchanged. Expression of Mub and MapA remained unchanged at pH 4.0, whilst expression of EF-Tu and plaA were up-regulated. Expression of MapA was down-regulated in the presence of 1.0 g/l l-cysteine HCl, suggesting that the gene is regulated by transcription attenuation that involves cysteine.

  20. Partial chemical characterization of antigenic preparations of chromoblastomycosis agents Caracterização química parcial de preparações antigênicas de agentes da cromoblastomicose

    Directory of Open Access Journals (Sweden)

    Tania Fraga BARROS

    1999-11-01

    Full Text Available Antigenic preparations (saline, methylic, metabolic and exoantigens of four agents of chromoblastomycosis, Fonsecaea pedrosoi, Phialophora verrucosa, Cladophialophora (Cladosporium carrionii and Rhinocladiella aquaspersa were obtained. Partial chemical characterization of these antigenic preparations was obtained by determination of the levels of total lipids, protein, and carbohydrates, and identification of the main sterols and carbohydrates. Methylic antigens presented the highest lipid contents, whereas metabolic antigens showed the highest carbohydrate content. Total lipid, protein, and carbohydrate levels were in the range of 2.33 to 2.00mg/ml, 0.04 to 0.02 mg/ml and 0.10 to 0.02 mg/ml, respectively, in the methylic antigens and in the range of 0.53 to 0.18mg/ml, 0.44 to 0.26mg/ml, and 1.82 to 1.02 mg/ml, respectively, in saline antigens. Total lipid, protein, and carbohydrate contents were in the range of 0.55 to 0.20mg/ml, 0.69 to 0.57mg/ml and 10.73 to 5.93mg/ml, respectively, in the metabolic antigens, and in the range of 0.55 to 0.15mg/ml, 0.62 to 0.20mg/ml and 3.55 to 0.42mg/ml, respectively, in the exoantigens. Phospholipids were not detected in the preparations. Saline and metabolic antigens and exoantigens presented hexose and the methylic antigen revealed additional pentose units in their composition. The UV light absorption spectra of the sterols revealed squalene and an ergosterol fraction in the antigens. The characterization of these antigenic preparations may be useful for serological evaluation of patients of chromoblastomycosis.Preparações antigênicas (antígenos salinos, metílicos, metabólicos e exoantígenos de quatro agentes da cromoblastomicose, Fonsecaea pedrosoi, Phialophora verrucosa, Cladophialophora (Cladosporium carrionii e Rhinocladiella aquaspersa foram obtidos e foi determinada a caracterização química parcial dos mesmos. Os antígenos metílicos apresentaram os maiores teores de lípides enquanto os

  1. A clinical study on the efficacy of natural therapeutic factors in Băile Tuşnad for the rehabilitation of post-stroke patients

    Directory of Open Access Journals (Sweden)

    Gabriela Dogaru

    2017-02-01

    Full Text Available Introduction. Stroke is one of the main causes of morbidity and mortality worldwide. Hypotonic carbonated mineral waters in Băile Tușnad are used for their peripheral and cerebral vasodilator effects in the prophylaxis, therapy and rehabilitation of cardiovascular patients. Objectives. The aim of the clinical study was to assess the efficacy of natural therapeutic factors in Băile Tușnad for continuing the rehabilitation of post-stroke patients in a spa and climatic resort for the treatment of cardiovascular diseases. Methods. The study included 30 patients with a history of stroke, aged between 56 and 89 years, with a mean age of 69 years, at the Facility Treatment of the Tușnad Spa Complex SA, in the period April-December 2014. The clinical study was a prospective longitudinal analysis. Of all 30 patients, 50% had ischemic stroke, 43% transient cerebral ischemic attack, and 7% hemorrhagic stroke. Hemiparesis was the most frequent clinical sign, followed by coordination, balance and gait disorders. Patients attended rehabilitation treatment consisting of kinesiotherapy, carbonated mineral water baths for 15 minutes, aerotherapy for 30 minutes daily, massotherapy, performed daily for 16 days. Each patient was clinically assessed before and after treatment based on the TINETTI Balance Scale, the 10-m walk test, the Motor Assessment Scale, the BARTHEL Index, adverse reactions. Results. At the end of treatment, an improvement in the walking speed, a statistically significant improvement in the quality of gait were observed, p < 0.05. Statistically significant results p<0.05 were also obtained for balance. On the Motor Assessment Scale, by comparing the means before and after treatment with the paired T test, a statistically significant value p<0.05 was obtained. When evaluating the patients’ performance for 10 activities of daily living depending on the need for external assistance, using the Barthel Index, the value of p <0.05 was

  2. Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles

    Directory of Open Access Journals (Sweden)

    Shiri M

    2016-11-01

    Full Text Available Mahdi Shiri,1,2,* Mona Navaei-Nigjeh,1,3,* Maryam Baeeri,1 Mahban Rahimifard,1 Hossein Mahboudi,4 Ahmad Reza Shahverdi,5 Abbas Kebriaeezadeh,1 Mohammad Abdollahi1,6,7 1Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, 2School of Medicine, Artesh University of Medical Sciences, 3Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; 4Department of Biotechnology, Faculty of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5Department of Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, 6Toxicology Interest Group, USERN, 7Endocrinology & Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran *These authors contributed equally to this work Abstract: Diazinon (DZ is an organophosphorus insecticide that acts as an acetylcholinesterase inhibitor. It is important to note that it can induce oxidative stress, lipid peroxidation, diabetic disorders, and cytotoxicity. Magnesium oxide (MgO and selenium nanoparticles (Se NPs showed promising protection against oxidative stress, lipid peroxidation, cytotoxicity, and diabetic disorders. Therefore, this study was conducted to explore the possible protective mechanisms of MgO and Se NPs against DZ-induced cytotoxicity in PaTu cell line. Cytotoxicity of DZ, in the presence or absence of effective doses of MgO and Se NPs, was determined in human pancreatic cancer cell line (PaTu cells after 24 hours of exposure by using mitochondrial activity and mitochondrial membrane potential assays. Then, the insulin, proinsulin, and C-peptide release; caspase-3 and -9 activities; and total thiol molecule levels were assessed. Determination of cell viability, including apoptotic and necrotic cells, was assessed via acridine orange/ethidium bromide double

  3. 《冥途飞脚》中的“义理与情”%The Rationalist and Emotion in Ming Tu Fei Jiao

    Institute of Scientific and Technical Information of China (English)

    赵黎慧

    2012-01-01

    Chikamatsu is Japan Edo Genroku period famous writer of doll and kabuki. Jing Yuan Xi He, Song Wei Ba Jiao and Chikamatsu are called Japan Genroku period of three literary giants. The work of Chika- matsu 《Ming Tu Fei Jiao》in Japan staged a number of the most popular work. In《Ming Tu Fei Jiao》, Zbong BingWei and Mei Chuan are in order to defend their love but to resist the morality of Edo Feudal.At the same time , they defend the credit which is set up by the Edo business community too.It is a expression that damage society common practice and business credit mood. The content of 《Ming Tu Fei Jiao》 show off the Japan Cul- ture "The Rationalist And Emotion". It is a new way for the friend exchange of Japan and China If we do un- derstand the The Rationalist And Emotion.%近松门左卫门是日本江户元禄时期有名的人形净琉璃和歌舞伎的剧作家,他与井原西鹤、松尾芭蕉一起被称为日本元禄时期的三大文学巨匠。近松门左卫门的作品《冥途飞脚》如今在日本是上演次数多、很受欢迎的作品。《冥途飞脚》中贫苦纯情的忠兵卫与梅川为了成就自己的爱情而抵抗江户封建社会道德,同时也抵抗了江户商业社会所建立起的信用组织,形成了破坏社会风气、破坏商业信用风气的一个实证。作品所表现的这种内容正是日本文化中“义理与情”矛盾与冲突的真实表现。

  4. IgG and IgG2 antibodies from cattle naturally infected with Anaplasma marginale recognize the recombinant vaccine candidate antigens VirB9, VirB10, and elongation factor-Tu

    Directory of Open Access Journals (Sweden)

    Flábio R Araújo

    2008-03-01

    Full Text Available Anaplasma marginale is an important vector-borne rickettsia of ruminants in tropical and subtropical regions of the world. Immunization with purified outer membranes of this organism induces protection against acute anaplasmosis. Previous studies, with proteomic and genomic approach identified 21 proteins within the outer membrane immunogen in addition to previously characterized major surface protein1a-5 (MSP1a-5. Among the newly described proteins were VirB9, VirB10, and elongation factor-Tu (EF-Tu. VirB9, VirB10 are considered part of the type IV secretion system (TFSS, which mediates secretion or cell-to-cell transfer of macromolecules, proteins, or DNA-protein complexes in Gram-negative bacteria. EF-Tu can be located in the bacterial surface, mediating bacterial attachment to host cells, or in the bacterial cytoplasm for protein synthesis. However, the roles of VirB9, VirB10, and TFSS in A. marginale have not been defined. VirB9, VirB10, and EF-Tu have not been explored as vaccine antigens. In this study, we demonstrate that sera of cattle infected with A. marginale, with homologous or heterologous isolates recognize recombinant VirB9, VirB10, and EF-Tu. IgG2 from naturally infected cattle also reacts with these proteins. Recognition of epitopes by total IgG and by IgG2 from infected cattle with A. marginale support the inclusion of these proteins in recombinant vaccines against this rickettsia.

  5. COST Action TU1206 "SUB-URBAN - A European network to improve understanding and use of the ground beneath our cities"

    Science.gov (United States)

    Campbell, Diarmad; de Beer, Johannes; Lawrence, David; van der Meulen, Michiel; Mielby, Susie; Hay, David; Scanlon, Ray; Campenhout, Ignace; Taugs, Renate; Eriksson, Ingelov

    2014-05-01

    Sustainable urbanisation is the focus of SUB-URBAN, a European Cooperation in Science and Technology (COST) Action TU1206 - A European network to improve understanding and use of the ground beneath our cities. This aims to transform relationships between experts who develop urban subsurface geoscience knowledge - principally national Geological Survey Organisations (GSOs), and those who can most benefit from it - urban decision makers, planners, practitioners and the wider research community. Under COST's Transport and Urban Development Domain, SUB-URBAN has established a network of GSOs and other researchers in over 20 countries, to draw together and evaluate collective urban geoscience research in 3D/4D characterisation, prediction and visualisation. Knowledge exchange between researchers and City-partners within 'SUB-URBAN' is already facilitating new city-scale subsurface projects, and is developing a tool-box of good-practice guidance, decision-support tools, and cost-effective methodologies that are appropriate to local needs and circumstances. These are intended to act as catalysts in the transformation of relationships between geoscientists and urban decision-makers more generally. As a result, the importance of the urban sub-surface in the sustainable development of our cities will be better appreciated, and the conflicting demands currently placed on it will be acknowledged, and resolved appropriately. Existing city-scale 3D/4D model exemplars are being developed by partners in the UK (Glasgow, London), Germany (Hamburg) and France (Paris). These draw on extensive ground investigation (10s-100s of thousands of boreholes) and other data. Model linkage enables prediction of groundwater, heat, SuDS, and engineering properties. Combined subsurface and above-ground (CityGML, BIMs) models are in preparation. These models will provide valuable tools for more holistic urban planning; identifying subsurface opportunities and saving costs by reducing uncertainty in

  6. Investigation of functional aspects of the N-terminal region of elongation factor Tu from Escherichia coli using a protein engineering approach

    DEFF Research Database (Denmark)

    Laurberg, M; Mansilla, Francisco; Clark, Brian F. C.

    1998-01-01

    importance for the factor to function properly. In this study, two lysines at positions 4 and 9 were mutated separately to alanine or glutamate. The resulting four point mutants were expressed and purified using the pGEX system. The untagged products were characterized with regard to guanine......The function of the N-terminal region of elongation factor Tu is still unexplained. Until recently, it has not been visible in electron density maps from x-ray crystallography studies, but the presence of several well conserved basic residues suggest that this part of the molecule is of structural......-nucleotide interaction, intrinsic GTPase activity, and binding of aminoacyl-tRNA (aa-tRNA). The results show that Lys9 is especially strongly involved in the association with guanine nucleotides and the binding of aa-tRNA. Also Lys4 plays a role in the association of GDP and GTP and is also of some importance in aa...

  7. Gigantes digitales. Quijote en 17000 tuits de Diego Buendía y Escribe tu propio Quijote de Belén Gache

    Directory of Open Access Journals (Sweden)

    Celia Corral Cañas

    2015-05-01

    Full Text Available To incorporate Don Quixote into the virtual environment offers many possibilities, from the literal adaptation into an electronic format, such as rewriting it on Twitter, to the allusion to the book in an avant-garde creation. Thus, among the various ways of tackling Don Quixote in Twitter, the project El Quijote en 17000 tuits by Diego Buendía, who makes a fragmented transcript in 17000 sections or tweets, stands out. Furthermore, Escribe tu propio Quijote by Belén Gache honors Cervantes’ novel with a simple interactive game that invites the reader to rethink the authorship issue within and outside the work. These two creations, born from the junction of the famous novel by Cervantes and the particularities of the new ecosystem, recover the quixotic nature to integrate it, from very different paths, into the digital universe.

  8. Comparison of two methods of femoral tunnel preparation in single-bundle anterior cruciate ligament reconstruction: a prospective randomized study Comparação entre dois métodos de preparação de túnel femoral na reconstrução do ligamento cruzado anterior em feixe único: estudo prospectivo randomizado

    Directory of Open Access Journals (Sweden)

    Qiang Zhang

    2012-08-01

    Full Text Available PURPOSE: To prospectively compare therapeutic effect of femoral tunnel preparation through the tibial tunnel and the anteromedial (AM portal in single-bundle anterior cruciate ligament (ACL reconstruction. METHODS: Between June 2008 and October 2010, 76 patients underwent single-bundle ACL reconstruction by autogenous grafting of semitendinosus and gracilis tendon. All cases were randomly divided into two groups according to the method of femoral tunnel preparation: transtibial (TT group (n=38 and anteromedial (AM group (n=38. Lysholm knee score and the KT-1000 anterior laxity at 30° of pre-and post-operation were assessed for two groups. RESULTS: Sixty-five patients (TT group, 34; AM group, 31 were followed up for more than 12 months, with a follow-up rate of 86%. The Lysholm knee score and the KT-1000 anterior laxity 12 months after operation were significantly better than before reconstruction. The Lysholm knee score and the KT-1000 anterior laxity were not significantly different between the TT and AM groups after operation. CONCLUSION: Femoral tunnel preparation through tibial tunnel or the anteromedial portal in single-bundle anterior cruciate ligament reconstruction shows same therapeutic effects.OBJETIVO: Comparar prospectivamente o efeito terapêutico da preparação do túnel femoral através do túnel tibial (TT ou da porta ântero-medial(AM na reconstrução do ligamento cruzado anterior(LCA em feixe único. MÉTODOS: Entre junho de 2008 e outubro de 2010, 76 pacientes foram submetidos à reconstrução do LCA em feixe único pelo enxerto autógeno de tendão semitendíneo egrácil.Todos os casos foram divididos aleatoriamente em dois grupos de acordo como método de preparação do túnel femoral: grupo transtibial (TT (n=38 e grupo ântero-medial (AM (n=38. Foi usado o escore Lysholm para joelho. O relaxamento anterior do joelho a 30° sob força tênsil de 133,32N foi determinado com o medidor KT-1000 no pré e no p

  9. Preliminary antigenic characterisation of an adult worm vomit preparation of Fasciola hepatica by infected human sera Caracterização antigênica preliminar de preparação de vômito de verme adulto de Fasciola hepatica por soros humanos infectados

    Directory of Open Access Journals (Sweden)

    María Alejandra De Almeida

    2007-02-01

    Full Text Available Fascioliasis is an emerging/re-emerging vector-borne disease with the widest known distribution. Approximately 17 million people are infected around the world, being the Andean region the most affected area. There is an important necessity to develop sensitive and specific diagnostic tools to treat patients early and to avoid complications. In this paper we evaluated the immune response of infected humans against two antigenic preparations: the total soluble extract (FhTSE and the adult worm vomit (FhAWV in order to identify antigenic fractions specific for Fasciola hepatica. Both preparations were processed by SDS-PAGE and Western blot with human sera with fascioliasis (F, other parasitosis and healthy individuals. In the immunoblot of FhTSE, sera F recognised 16 bands with MW between eight and 110 kDa, from which those of 8, 9, 10, 38, 45 and 57 kDa were specific. In the preparation FhAWV, sera F recognised nine bands with MW from eight to 85 kDa, from which those of 8, 12, 15 and 24 kDa were specific. Some bands of cross-reaction were evident with sera from patients with other parasitoses, more frequent with the FhTSE. Bands within the MW mentioned, particularly that of eight kDa, have been shown to be specific by others, and deserve additional characterisation for their potential use in immunodiagnosis.Fasciolíase é uma doença emergente/re-emergente transmitida por vetores com a distribuição sabidamente mais ampla. Existem aproximadamente 17 milhões de pessoas infectadas em todo mundo, sendo a região andina a área mais afetada. Há uma necessidade importante para desenvolver ferramentas diagnósticas sensíveis e específicas para tratar cedo os pacientes e para evitar complicações. Neste trabalho avaliamos a resposta imune de seres humanos infectados comparando a duas preparações antigênicas: o extrato solúvel total (FhTSE e o vômito (FhAWV do verme adulto a fim de identificar as frações antigênicas específicas para

  10. 碳含量对TU5JP4灰铸铁冷激凸轮轴铸造弯曲变形和气孔的影响%Effect of Carbon Content on Bending Deformation and Porosity of TU5JP4 Chilled Grey Cast Iron Camshaft

    Institute of Scientific and Technical Information of China (English)

    沈保罗; 李莉; 张昊

    2012-01-01

    本文采用生产现场的大量数据,统计分析了碳含量对TU5JP4灰铸铁冷激凸轮轴弯曲变形和气孔的影响.统计数据表明,碳含量从3.4%~3.6%提高到3.6%~3.7%之间,可以较明显地降低凸轮轴的过度弯曲变形(跳动超过1mm)和气孔缺陷.用户机械加工的统计数据表明,碳含量提高后,因皮下气孔导致的废品率下降约30%.%The production data on spot have been used for statistical analysis of effect of the carbon content on bending deformation and blowhole defect of TU5JP4 chilled cast iron camshaft. It showed that increasing carbon content of the camshaft from 3.4%~3.6% to 3.6%~3.7%, has significantly reduced the bending deformation of the camshaft (radial runout more than lmm) and porosity defect. The statistics of the user showed that as the carbon content increased, the waste rate caused due to the blowhole defect decreased by about 30%.

  11. Optimization of liquid state fermentation conditions for cellulase production by Bacillus amyloliquefaciens strain Tu-115 with filter paper enzyme as index%以滤纸酶活力为指标优化解淀粉芽孢杆菌Tu-115菌株产纤维素酶液体发酵条件

    Institute of Scientific and Technical Information of China (English)

    姜军坡; 朱宝成; 王世英

    2014-01-01

    以滤纸酶活力为指标对解淀粉芽孢杆菌(Bacillus amyloliquefaciens)Tu-115菌株产纤维素酶液体发酵条件进行优化.通过单因素和正交试验相结合的方法,考察培养基中碳源、氮源、起始pH值、装瓶量、温度、接种量、发酵时间等发酵条件对产酶能力的影响.结果表明,当培养条件为葡萄糖4%、黄豆饼粉3%、培养基起始pH值4.0、接种量4%、装瓶量50 ml/250 ml,37℃发酵72 h时,发酵液中滤纸酶活力达到最高值,为3 309.90 U/ml,比优化前提高了125倍.优化后,Tu-115菌株产滤纸酶能力大大提高,达到了预期目的.

  12. Assessment of protocols for surgical-site preparation in a regional network of hospitals Evaluación de la normalización de la preparación prequirúrgica en una red regional de hospitales Avaliação da normatização da preparação pré-cirúrgica em uma rede regional de hospitais

    Directory of Open Access Journals (Sweden)

    Maria Dolores Peñalver-Mompeán

    2012-04-01

    en la mayoría de hospitales, pero la calidad de los protocolos es deficiente, así como la normalización de prácticas basadas en evidencia.A infecção do sítio cirúrgico é evento adverso, possível de prevenir mediante a implementação de boas práticas de preparação pré-cirúrgica. Este estudo teve por objetivo descrever a existência e qualidade da protocolização da preparação pré-cirúrgica em uma rede regional de hospitais públicos de Múrcia, Espanha. Avaliou-se o indicador Existência de protocolo/norma de preparação pré-cirúrgica, analisando-se a qualidade formal (atributos e de conteúdo (presença de recomendações baseadas em evidência dos documentos existentes. Sete (de nove hospitais comprovaram que tinham protocolos de preparação pré-cirúrgica. Identificaram-se oportunidades de melhoria da qualidade formal e de conteúdo dos protocolos. As recomendações sobre assepsia estavam incompletas na maioria dos documentos, e aquelas de eliminação do pelo mostraram-se contrárias à evidência. A preparação pré-cirúrgica está protocolizada na maioria dos hospitais, mas a qualidade dos protocolos é deficiente, assim como a padronização das práticas baseadas em evidência.

  13. Análise do processo de preparação da produção no desenvolvimento de novos produtos por meio de um estudo de caso em uma empresa do setor siderúrgico Production preparation process analysis in new products development - a case study in the steel industry

    Directory of Open Access Journals (Sweden)

    Angelo Varandas Junior

    2012-04-01

    Full Text Available A demanda por novos produtos tem aumentado em algumas empresas do setor siderúrgico, indicando uma crescente preocupação com o desempenho do processo de desenvolvimento de produtos (PDP, que depende de vários elementos conceituais importantes apontados na literatura. Em uma empresa siderúrgica, um fator relevante é o projeto do processo de produção, devido à característica de ser um processo de fabricação contínuo e por, geralmente, envolver investimentos significativos na fase de preparação da produção. Nesse contexto, o presente trabalho busca realizar uma análise desta fase em uma empresa siderúrgica. Pretende-se, por meio da abordagem metodológica de estudo de caso, identificar práticas organizacionais, focando nos aspectos operacionais e suas interfaces, envolvendo as áreas funcionais de marketing, engenharia do produto e manufatura. Também é tratada no trabalho a integração dos times multidisciplinares, a tomada de decisão e os critérios para avaliação dos resultados do PDP. O trabalho conclui que a empresa analisada utiliza-se de práticas que estruturam a preparação da produção e que a integração interfuncional das áreas é o que se destaca para o sucesso do PDP.The demand for new products has increased in some companies from the steel industry, indicating a growing concern with the performance of products development process (PDP, which depends on several important conceptual elements found in the literature. The production process project is a relevant factor in a steel company, because it is a continuous manufacturing process and it generally involves significant investments in the production preparation phase. In this context, the present paper analyzes this stage of the process in a steel producer. The research aims to identify, through a case study methodological approach, the company's organizational practices with focus on the operational aspects and their interfaces involving the functional

  14. Comparação entre os sistemas rotatórios Pathfile + Protaper Universal e Twisted Files quanto à preservação da morfologia e ao tempo de trabalho despendido na preparação de canais curvos

    Directory of Open Access Journals (Sweden)

    Daniel Guimarães Pedro Rocha

    Full Text Available INTRODUÇÃO: Instrumentos rotatórios em níquel-titânio (NiTi reduzem erros processuais e o tempo requerido para a preparação do canal radicular. OBJETIVO: Comparar dois sistemas de instrumentação rotatória NiTi - Pathfile + ProTaper Universal e Twisted Files - quanto à capacidade de preservação da morfologia do canal e quanto ao tempo de trabalho necessário para a preparação de canais curvos. MATERIAL E MÉTODO: Os canais mesiais de 32 molares inferiores humanos (com ângulos de curvatura entre 20 e 40 graus foram selecionados para o estudo. As amostras foram divididas aleatoriamente em dois grupos de 16 canais. Os preparos foram realizados por um Endodontista, utilizando-se a combinação dos instrumentos rotatórios Pathfile e ProTaper Universal (grupo PT e os instrumentos rotatórios Twisted Files (grupo TF. Três Endodontistas analisaram imagens com a técnica da dupla radiografia digital, pré e pós-instrumentação, para avaliar se ocorreu transporte apical e/ou aberrações na morfologia dos canais. Por meio de uma análise computadorizada e com o auxílio de magnificação, as imagens inicial e final do canal radicular foram comparadas por meio de sua sobreposição. O teste de odds ratio foi utilizado para a comparação dos níveis de preservação da morfologia do canal, e ANOVA, complementada pelo teste de Tukey, foi utilizada para a comparação dos tempos de preparo. O nível de significância adotado foi de 5%. RESULTADO: Ocorreram desvios na trajetória do canal em ambos os grupos, mas a diferença entre estes não foi significativa. O tempo de trabalho requerido pela combinação dos sistemas Pathfile + ProTaper Universal foi significativamente maior do que o requerido pelo sistema Twisted Files. CONCLUSÃO: Os dois sistemas apresentaram performances semelhantes em relação à preservação da trajetória do canal; no entanto, o tempo de trabalho requerido pelo sistema Twisted Files foi menor.

  15. Divdaivu Ginka (Ginkgo Biloba) farmakoloģiskās īpašības un tā preparātu aprite SIA "Gulbju aptiekā"

    OpenAIRE

    Indriča, Alīna

    2014-01-01

    Divdaivu ginks (Ginkgo biloba) ir viens no vecākajiem kokiem pasaulē. Ķīnas tradicionālajā medicīnā ginka lapas un sēklas izmanto jau vismaz 5000 gadus. Standartizēts Ginkgo biloba ekstrakts satur – 24% flavonglikozīdu un 6% terpēnlaktonu. Šī darba mērķis ir veikt apkopoto datu analīzi par Ginkgo biloba preparātu apriti „Gulbju aptiekā” – Gulbenē, 2013. gada laikā, kā arī noskaidrot cilvēku informētību par Ginkgo biloba preparātu lietošanu. Darbā tika izvirzīti 4 uzdevumi: Noskaidrot, k...

  16. EFECTO DEL PROGRAMA “MUÉVETE POR TU SALUD” SOBRE LA COMPOSICIÓN CORPORAL, PRESIÓN ARTERIAL, CALIDAD DE SUEÑO Y ESTRÉS ACADÉMICO EN ESTUDIANTES RESIDENTES DE UNA UNIVERSIDAD PRIVADA CONFESIONAL

    OpenAIRE

    Salinas Arias, Saulo; Universidad Peruana Unión Filial Tarapoto; Pérez Rivera, Jessica; Castro Rodríguez, Elda

    2016-01-01

    universitaria como parte del cumplimiento de la reforma pro salud. Objetivo: Determinar laeficiencia del programa “Muévete por tu salud” en la composición corporal, presión arterial,calidad de sueño y estrés académico en los estudiantes residentes UPeU sede Tarapoto.Método: Se desarrolló el programa de actividad física denominado “Muévete por tu salud”con 79 estudiantes (F59 – M20) entre las edades de 16 y 19 años que residen en el campusuniversitario. Las variables consideradas fueron: Compo...

  17. A utilização do sistema de preparação de textos LaTeX na produção de textos acadêmicos no Brasil: uma investigação preliminar e perspectivas

    Directory of Open Access Journals (Sweden)

    Youssef Alvarenga Cherem

    2015-06-01

    Full Text Available Introdução: LaTeX é um sistema de preparação eletrônica de textos baseado no sistema tipográfico TeX. Neste artigo, apontamos os principais benefícios e desafios de utilização dessa ferramenta, bem como perspectivas para utilização a curto e médio prazo no Brasil.Objetivo: analisar o uso do software LaTeX na produção de textos acadêmicos no Brasil.Metodologia: Revisão bibliográfica e análise documental.Resultados: A utilização de uma linguagem de mark-up como LaTeX pode trazer benefícios tanto do ponto de vista do produto final e sua relação com o leitor, quanto do ponto de vista do processo de edição do texto. Apesar disso, seu uso no Brasil é bastante restrito.Conclusões: Um dos maiores desafios para a disseminação de seu uso é a falta de apoio institucional oficial na área acadêmica e científica. Sua aceitação institucional, bem como iniciativas de capacitação do usuário, são apontados como fatores  que podem mudar esse quadro.

  18. Study on need and importance of Information Literacy in Nepal: Special emphasis to students of MLisc, TU, Central Department of Library and Information Science and professionals of Kathmandu Valley

    OpenAIRE

    Shrestha, Saroja

    2005-01-01

    The present study is a study on "Need and importance of information literacy in Nepal: special emphasis to students of MLISc, Central Department of Library and Information Science, TU and professional librarians of Kathmandu". In the present context where emphasis is given more on getting the accurate information at the needed time. The professionals working as the information providers should be literate. Librarians are the primary information providers so professional librarians and student...

  19. 《古今图书集成》医学思想研究%Study on Medical Thoughts inGu Jin Tu Shu Ji Cheng

    Institute of Scientific and Technical Information of China (English)

    杨威; 阎卫红; 余丞浩; 于峥

    2016-01-01

    Gu Jin Tu Shu Ji Cheng is a large-scale reference book in the Qing Dynasty, which is known as the ancient "Encyclopedia of China". Its content of traditional Chinese medicine is rich. Gu Jin Tu Shu Ji Cheng Yi Bu Quan Lu quoted over 120 ancient medical books covering principles of medical science, the four methods of diagnosis, clinical departments, and medical records. Other compilations and scriptures also include large amounts of medical content. The books are widely cited and precious. The contents of this book are selected finely, incorporate things of diverse nature, include large numbers of classics, and conduct clarification, with a purpose to search and retrieve; the contents also pay attention to the clinical practice, pay equal attention to the four methods of diagnosis, refer to syndrome differentiation and treatment, and include methodology and discussion, with unique views and clear logic, which can help readers’ learning.%《古今图书集成》系清代大型类书,被誉为古代“中国百科全书”,书中医学内容丰富。其中,《古今图书集成·医部全录》征引历代重要医籍120余种,涵盖医理、四诊、临床各科、医药纪事等;其他汇编、典部也有大量与医学相关的内容,征引广泛,弥足珍贵。书中对收录医学内容挑选精细,兼收并蓄,经典赅备,分门别类,便于检索阅读;重视临床实用,四诊并重,证治相参,方论俱备,观点独到,条理清晰,便于读者学习。

  20. Numerical modelling of GPR ground-matching enhancement by a chirped multilayer structure - output of cooperation within COST Action TU1208

    Science.gov (United States)

    Baghdasaryan, Hovik V.; Knyazyan, Tamara M.; Hovhannisyan, Tamara. T.; Marciniak, Marian; Pajewski, Lara

    2016-04-01

    As is well know, Ground Penetrating Radar (GPR) is an electromagnetic technique for the detection and imaging of buried objects, with resolution ranging from centimeters to few meters [1, 2]. Though this technique is mature enough and different types of GPR devices are already in use, some problems are still waiting for their solution [3]. One of them is to achieve a better matching of transmitting GPR antenna to the ground, that will increase the signal penetration depth and the signal/noise ratio at the receiving end. In the current work, a full-wave electromagnetic modelling of the interaction of a plane wave with a chirped multilayered structure on the ground is performed, via numerical simulation. The method of single expression is used, which is a suitable technique for multi-boundary problems solution [4, 5]. The considered multilayer consists of two different dielectric slabs of low and high permittivity, where the highest value of permittivity doesn't exceed the permittivity of the ground. The losses in the ground are suitably taken into account. Two types of multilayers are analysed. Numerical results are obtained for the reflectance from the structure, as well as for the distributions of electric field components and power flow density in both the considered structures and the ground. The obtained results indicate that, for a better matching with the ground, the layer closer to the ground should be the high-permittivity one. Acknowledgement This work benefited from networking activities carried out within the EU funded COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar" (www.GPRadar.eu, www.cost.eu). Part of this work was developed during the Short-Term Scientific Mission COST-STSM-TU1208-25016, carried out by Prof. Baghdasaryan in the National Institute of Telecommunications in Warsaw, Poland. References [1] H. M. Jol. Ground Penetrating Radar: Theory and Applications. Elsevier, 2009. 509 pp. [2] R. Persico. Introduction to

  1. Pilsētu ilgtspējīgas attīstības plānošanas un pārvaldīšanas aspekti mūsdienās

    OpenAIRE

    Grizāns, J; Vanags, J.

    2010-01-01

    Zinātniskā raksta mērķis ir pilsētu ilgtspējīgas attīstības plānošanas un pārvaldīšanas aspektu izpēte mūsdienu urbanizācijas un globalizācijas procesu apstākļos. Nospraustā pētījuma mērķa sasniegšanai ir izmantotas šādas pētnieciskās izziņas metodes: pilsētu attīstību regulējošo normatīvo dokumentu un konceptuālo pamatnostādņu analīze, ekspertu vērtējumu izpēte, kā arī monogrāfiskās un loģiskās pieejas metodes. Pētījuma rezultātā ir izstrādāti konceptuālie priekšlikumi pilsētu sociāli ekonom...

  2. Presentation of the new Department of Drilling, Special Deep Drilling Equipment and Mining Machinery of the Institute of Drilling and Fluid Mining of TU Bergakademie Freiberg University; Vorstellung des neuen Lehrstuhls fuer Bohrtechnik, Spezialtiefbauausruestungen und Bergbaumaschinen am Institut fuer Bohrtechnik und Fluidbergbau der TU Bergakademie Freiberg

    Energy Technology Data Exchange (ETDEWEB)

    Reich, M.; Ksienzyk, F. [TU Bergakademie Freiberg (Germany)

    2007-09-13

    The paper presents the new Department of Drilling, Special Deep Drilling Equipment and Mining Machinery of the Institute of Drilling and Fluid Mining of TU Bergakademie Freiberg University. The new combination of drilling technology and mechanical engineering reflects the rapid technological development of deep drilling during the past two decades. Teaching subjects and research activities are described. Although trends are positive, there are still not enough students of drilling and fluid mining. If the rapidly increasing demand for engineers specializing in drilling technology and petroleum and natural gas production is to be met on a medium-term basis, joint efforts of industry and universities will be necessary as discussed in this paper. (orig.)

  3. La satisfacción de residentes con su colonia y el programa gubernamental Tu Casa en el estado de Zacatecas

    Directory of Open Access Journals (Sweden)

    Jorge Ibarra Salazar

    2016-01-01

    Full Text Available El grado de satisfacción residencial, considerado como un indicador de nivel de vida, tiene como uno de sus componentes la satisfacción con la colonia de los individuos. En este artículo estimamos una serie de modelos para analizar el grado de satisfacción con la colonia de una muestra de hogares en el estado de Zacatecas. Las estimaciones nos permiten determinar si el programa federal de vivienda Tu Casa en ese estado, operado de 2004 a 2009, tuvo alguna influencia en el grado de satisfacción con la colonia, de acuerdo con la percepción de los residentes entrevistados. Encontramos evidencia de que el referido programa causó un efecto significativo en el grado de satisfacción del grupo de familias beneficiarias. Además de ese resultado, la estimación por mínimos cuadrados ordinarios muestra que el acceso vial, la contaminación ambiental, la percepción del valor de la casa y el tiempo de vivir en la vivienda influyen de forma significativa en el grado de satisfacción con la colonia. En los modelos estimados por probit y logit encontramos evidencia de que la seguridad pública y la posesión del inmueble también influyen en tal satisfacción.

  4. CERN Library | Sandrine Saison-Marsollier, Corinne Pralavorio and Michel Spiro present "Si tu devais me dessiner l’Univers…" | 10 December

    CERN Multimedia

    CERN Library

    2015-01-01

    Sandrine Saison-Marsollier, Corinne Pralavorio and Michel Spiro present Si tu devais me dessiner l’Univers… Thursday, 10 December 2015 at 3.30 p.m. at the Library, Building 52-1-052 Tea and coffee will be served at 3 p.m. In 2014, CERN launched an art competition for local primary schools. Children were asked to come up with questions about the universe, matter and working as a researcher. The initiative was so successful that it has been turned into a book that teachers can explore with their pupils, discussing the questions and discovering the answers together. The book is laid out in double-page spreads, with the child’s illustration on one page and the question and its answer opposite. The answers take the form of a short text written by Michel Spiro, the competition’s scientific advisor, together with a cultural quotation whose purpose is to highlight the close association between science, literature and philosophy. The questions include: What was there...

  5. Las revistas de tu casa: una provocación para innovar en la difusión académica

    Directory of Open Access Journals (Sweden)

    Mauricio Andión Gamboa

    2005-01-01

    Full Text Available Desde principios del siglo pasado, las universidades latinoamericanas han mostrado una vocación “extensionista”, es decir, un afán de difusión de la cultura científica y humanística más allá de los confines de las comunidades académicas. Las revistas y los libros han sido tradicionalmente la principal vía que las universidades han utilizado para difundir su producción cultural entre sus comunidades y la sociedad en general. La Galaxia de Gutenberg es sumamente extensa y, aún hoy, las universidades no han terminado de cruzarla. Las revistas universitarias son más que un medio de difusión, de hecho son proyectos académicos, la mayoría de ellos, que aglutinan grupos de interés integrados por afinidad ideológica, política, social o cultural. Para propiciar la discusión, se elaboró un video documental titulado “Las revistas de tu casa” que aborda la problemática actual de las revistas en la Universidad Autónoma Metropolitana (UAM, en su conjunto, y dentro del cambio de paradigma tecnológico.

  6. Incidence and remission of parasomnias among adolescent children in the Tucson Children’s Assessment of Sleep Apnea (TuCASA Study

    Directory of Open Access Journals (Sweden)

    Furet O

    2011-06-01

    Full Text Available Background: Longitudinal assessments of parasomnias in the adolescent population are scarce. This analysis aims to identify the incidence and remission of parasomnias in the adolescent age group.Methods: The TuCASA study is a prospective cohort study that initially enrolled children between the ages of 6 and 11 years (Time 1 and subsequently re-studied them approximately 5 years later (Time 2. At both time points parents were asked to complete a comprehensive sleep habits questionnaire designed to assess the severity of sleep-related symptoms that included questions about enuresis (EN, sleep terrors (TR, sleep walking (SW and sleep talking (ST.Results: There were 350 children participating at Time 1 who were studied as adolescents at time 2. The mean interval between measurements was (4.6 years. The incidence of EN, TR, ST, and SW in these 10-18 year old children was 0.3%, 0.6%, 6.0% and 1.1% respectively. Remission rates were 70.8%, 100%, 64.8% and 50.0% respectively.Conclusions: The incidence rates of EN, TR, and SW were relatively low moving from childhood to adolescence while remission rates were high across all parasomnias.

  7. Tu Amigo Pepe: Evaluation of a Multi-media Marketing Campaign that Targets Young Latino Immigrant MSM with HIV Testing Messages.

    Science.gov (United States)

    Solorio, Rosa; Norton-Shelpuk, Pamela; Forehand, Mark; Montaño, Daniel; Stern, Joshua; Aguirre, Joel; Martinez, Marcos

    2016-09-01

    Latino immigrant men who have sex with men (MSM) are at risk for HIV and delayed diagnosis in the United States. This paper describes the evaluation of a pilot of the Tu Amigo Pepe, a multimedia HIV testing campaign aimed at Latino MSM in Seattle, WA particularly targeting immigrants who may not identify as gay, ages 18-30 years old. The 16-week campaign included Spanish-language radio public service announcements (PSAs), a Web site, social media outreach, a reminder system using mobile technology, print materials and a toll-free hotline. In developing the PSAs, the Integrated Behavioral Model was used as a framework to reframe negative attitudes, beliefs and norms towards HIV testing with positive ones as well as to promote self-efficacy towards HIV testing. The campaign had a significant and immediate impact on attitudes, beliefs, norms and self-efficacy towards HIV testing as well as on actual behavior, with HIV testing rates increasing over time.

  8. Tu e o de quem?" Tecendo os os com Bakhtin, Vigotski e Paulo Freire / Who do you think you are?" Commenting the wires with Bakhtin, Vigotski and Freire

    Directory of Open Access Journals (Sweden)

    Joselito Manoel de Jesus

    2010-08-01

    Full Text Available RESUMO: Este artigo analisa o enunciado "Tu é o de quem?", apontando para a responsabilidade que um filho da roça tem como partícipe do sustento da família e como essa realidade imediata constitui fios que enredam sua existência. Adota as contribuições de Bakhtin, Freire e Vigotski, evidenciando o processo de humanização como resultado das interações verbais e sociais que os indivíduos estabelecem em determinadas condições que caracterizam o espaço e o tempo em queestão inseridos e inserindo-se num processo ininterrupto. ABSTRACT:This article aims at analyzing the statement addressed to a farm child "Who do you think you are?" pointing to the fact that these children are responsible for their family income and as thisimmediate reality is intertwined by wires which build up their existence. This text is based on Bakhtin, Vygotsky and Freire, showing the process of humanization as a result of social and verbalinteractions which individuals establish under certain conditions in order to characterize the space and time in which they are inserted and inserting themselves into a never-ending process.

  9. O efeito facilitatório do óxido nítrico sobre a transmissão neuromuscular de preparações nervo frênico- diafragma isolado de ratos é Ca++ dependente - DOI: 10.4025/actascibiolsci.v26i4.1528 NO- induced neuromuscular facilitation in the phrenic nerve diaphragm preparation of rats is Ca++ dependent - DOI: 10.4025/actascibiolsci.v26i4.1528

    Directory of Open Access Journals (Sweden)

    Heraldo Esheveria Borges

    2004-04-01

    Full Text Available A pesquisa foi conduzida para verificar se os efeitos neuromusculares induzidos por óxido nítrico (NO poderiam depender dos níveis extracelulares de Ca++. O doador de NO, nitroprussiato de sódio (NPS, e o análogo de GMPc, 8-Br-cGMP, aumentaram a amplitude das contrações musculares (ACM em preparações indiretamente estimuladas a 0.2 Hz, mas não produziram efeitos quando a solução nutriente (Krebs normal foi trocada por Krebs com baixo Ca++/ alto Mg++. NPS e 8-Br-cGMP reduziram a ACM quando as preparações foram diretamente estimuladas, mas tal efeito não foi observado com o uso de Krebs com baixo Ca++/ alto Mg++. Dados sugerem que os efeitos neuromusculares induzidos por NO dependem dos níveis extracelulares de Ca++The research was carried out to verify whether the neuromuscular effects induced by nitric oxide (NO might depend on extracellular level of Ca++. The donor of NO, sodium nitroprusside (SNP, and the analogue of cGMP, 8-Br-cGMP, increased the muscular contraction amplitude (MCA in preparations indirectly stimulated at 0.2 Hz, but did not produce any effect when the nutrient solution was exchanged by Krebs buffer low Ca++/ high Mg++. SNP and 8-Br-cGMP reduced the MCA in preparations directly stimulated. Such effect was not recorded in Krebs buffer low Ca++/ high Mg++. Data suggest that the neuromuscular effects induced by NO or cGMP depend on extracellular level of Ca++

  10. Porcionamento dos principais alimentos e preparações consumidos por adultos e idosos residentes no município de São Paulo Portion sizes of the main foods and preparations consumed by adults and elderly living in the city of São Paulo, Brazil

    Directory of Open Access Journals (Sweden)

    Jackeline Venancio Carlos

    2008-08-01

    Full Text Available OBJETIVO: Identificar o tamanho das porções dos alimentos e das preparações mais consumidas ou que mais contribuem com o valor energético total da dieta de adultos e idosos. MÉTODOS: Realizou-se inquérito domiciliar de base populacional em 2003, com amostra representativa de 1 477 indivíduos acima de 20 anos residentes no município de São Paulo. O consumo alimentar foi verificado pelo recordatório de 24 horas, digitado no programa Nutrition Data System. Os alimentos e as preparações selecionados para averiguação da porção foram os consumidos por, no mínimo, 10% da população de estudo ou que contribuíram com até 80% do valor energético total. As porções médias, obtidas pelo percentil 50, foram comparadas segundo sexo e estado nutricional, por meio do teste de Kruskal Wallis. RESULTADOS: Arroz e feijão foram os alimentos mais consumidos e que mais contribuíram para o valor energético da dieta. Os homens consumiram maior porção destes alimentos, quando são comparados às mulheres. Observou-se que a porção de leite está abaixo do estipulado pelo Guia Alimentar Brasileiro, porém a maior diferença encontrada foi em relação à alface e ao tomate. Verificou-se que indivíduos com excesso de peso consomem maiores porções de peito de frango. CONCLUSÃO: O tamanho das porções de alguns alimentos é maior entre homens, porém, na prática, não há como diferenciar, em medidas caseiras, valores tão próximos como os encontrados na maioria dos alimentos. As porções dos principais alimentos dos grupos de verduras e legumes e leite e derivados são menores que o proposto pelo Guia Alimentar.OBJECTIVE: Identify the portion sizes of the most consumed foods and preparations or those that contribute most to the total energy of the diet of adults and seniors. METHODS: A population-based household survey with a representative sample of 1 477 individuals older than 20 years living in the city of São Paulo was done in

  11. Tetanic fade induced by d-Tubocurarine, Hexamethonium and Neostigmine in phrenic nerve diaphragm of diabetic rats Fadiga neuromuscular induzida por d-Tubocuranina, Hexametônio e Neostigmina em preparações nervo frênico-diafragma isolado de ratos diabéticos

    Directory of Open Access Journals (Sweden)

    Wilson Alves do Prado

    1998-07-01

    Full Text Available A acetilcolina liberada do terminal nervoso motor (TNM pode modular sua própria liberação (automodulação do TNM, interagindo com receptores nicotínicos (autoestimulação do TNM ou muscarínicos (autoinibição do TNM pré-juncionais. Por outro lado, tem-se demonstrado que a neuropatia induzida pelo estado diabético determina vários danos estruturais no interior do TNM, sem, contudo, interferir na velocidade e na integridade da transmissão neuromuscular. Estudos farmacológicos demonstram que animais diabéticos, quando comparados aos normais, são menos sensíveis a alguns bloqueadores neuromusculares (d-tubocurarina, galamina, pancurônio e decametônio. Esses resultados sugerem que alguma modificação no sistema de automodulação do TNM pode contrabalançar as deficiências neuronais induzidas pelo estado diabético. Dessa forma, o presente estudo foi conduzido com preparações nervo frênico-diafragma isolado de ratos (obtidas de animais normais e diabéticos na tentativa de verificar se existiriam diferenças na fadiga neuromuscular induzida por drogas (d-tubocurarina, neostigmina, hexametônio. Nossos resultados mostraram que, embora não existissem diferenças na indução da fadiga neuromuscular induzida por d-tubocurarina, neostigmina ou hexametônio, o recobro da fadiga neuromuscular induzida por d-tubocurarina foi mais rápido em preparações neuromusculares obtidas de animais diabéticos. Essa diferença pode estar relacionada a alguma modificação induzida pelo estado diabético que determinou redução da afinidade da d-tubocurarina para os receptores nicotínicos pré-juncionais.The acetylcholine released from motor nerve terminal (MNT can regulate its own output (MNT automodulation acting on nicotinic (positive feed back automodulation or muscarinic (negative feedback automodulation presynaptic receptors. On the other hand, diabetic neuropathy, a disorder of peripheral nerves, is one of the most common complication

  12. Rendimento e comportamento espectrofotométrico da lignina extraída de preparações de parede celular, fibra em detergente neutro ou fibra em detergente ácido Yield and spectrophotometric pattern of lignin extracted from cell wall, neutral detergent fiber or acid detergent fiber

    Directory of Open Access Journals (Sweden)

    Nancy Maria de Fátima Savioli

    2000-08-01

    Full Text Available A lignina extraída da planta forrageira pelo reagente brometo de acetila (lignina brometo de acetila - LBrAc, para ser empregada como padrão de referência no método espectrofotométrico para a determinação quantitativa da lignina, não pode originar-se da planta intacta, devido à interferência de diversas outras substâncias, que absorvem no mesmo comprimento de onda que a lignina; aconselha-se, portanto, que a extração da lignina seja realizada a partir de um dos seguintes compostos: parede celular propriamente dita (PC, fibra em detergente neutro (FDN ou fibra em detergente ácido (FDA. Os rendimentos das extrações das ligninas, bem como o comportamento espectrofotométrico das mesmas, foram avaliados nestes três compostos provenientes de diversas plantas forrageiras. Na média, os rendimentos das extrações de LBrAc foram relativamente uniformes entre si, entretanto, como a concentração de FDA foi a menor entre todas as preparações (devido principalmente à solubilização da hemicelulose e como esta não apresentou maior capacidade extrativa da lignina, o rendimento de LBrAc proveniente da FDA, com base na planta inteira, foi o menor observado. A absorção da luz ultravioleta das diversas ligninas foi desigual, revelada pelos espectrogramas traçados, de tal modo a sugerir que a preparação de parede celular adotada para o isolamento da lignina deve ser a mesma para a determinação final da concentração de lignina.The lignin extracted from the forages by the acetyl bromide reagent (acetyl bromide lignin - AcBrL to be used as a reference standard for the quantitative spectrophotometric determination of lignin could not originate from intact plant because of interference of other substances, which absorb ultraviolet light at the same wavelength of lignin. It is then recommended that lignin extraction be performed in one of the following materials: cell wall itself (CW, neutral detergent fiber (NDF, or acid detergent

  13. The influence of lidocaine and racemic bupivacaine on neuromuscular blockade produced by rocuronium: a study in rat phrenic nerve-diaphragm preparation Influência da lidocaína e da bupivacaína racêmica no bloqueio neuromuscular produzido pelo rocurônio: estudo em preparação nervo frênico-diafragma de rato

    Directory of Open Access Journals (Sweden)

    Vanessa Henriques Carvalho

    2009-06-01

    Full Text Available PURPOSE: To evaluate in vitro lidocaine and racemic bupivacaine effects in neuromuscular transmission and in neuromuscular blockade produced by rocuronium. METHODS: Rats were distributed in 5 groups (n = 5 in agreement with the studied drugs: lidocaine, racemic bupivacaine, rocuronium, separately (Groups I, II, III; rocuronium in preparations exposed to local anesthetics (Groups IV, V. The concentrations used were: 20 µg/mL, 5 µg/mL and 4 µg/mL, for lidocaine, bupivacaine and rocuronium, respectively. It was evaluated: 1 amplitude of diaphragm muscle response to indirect stimulation, before and 60 minutes after separately addition of lidocaine, racemic bupivacaine and rocuronium and the association of local anesthetics - rocuronium; 2 membrane potentials (MP and miniature end-plate potentials (MEPP. RESULTS: Lidocaine and bupivacaine separately didn't alter the amplitude of muscle response and MP. In preparations previously exposed to lidocaine and racemic bupivacaine, the rocuronium blockade was significantly larger (90.10 ± 9.15% and 100%, respectively, in relation to the produced by rocuronium separately (73.12 ± 9.89%. Lidocaine caused an increase in the frequency of MEPP, being followed by blockade; racemic bupivacaine produced decrease being followed by blockade. CONCLUSIONS: Local anesthetics potentiated the blockade caused by rocuronium. The alterations of MEPP identify presynaptic action.OBJETIVO: Avaliar in vitro os efeitos da lidocaína e bupivacaína racêmica na transmissão neuromuscular e no bloqueio neuromuscular produzido pelo rocurônio. MÉTODOS: Ratos foram distribuídos em 5 grupos (n = 5 de acordo com a droga estudada: lidocaina, bupivacaína racêmica, rocurônio, isoladamente (Grupos I, II, III; rocurõnio em preparações expostas aos anestésicos locais (Grupos IV, V. As concentrações utilizadas foram: 20 µg/mL, 5 µg/mL e 4 µg/mL, para lidocaína, bupivacaína e rocurônio, respectivamente. Avaliou-se: 1

  14. Avaliação de uma nova microtécnica de fixação de complemento (Garcia & Sotelo, 1991 para o diagnóstico da doença de Chagas crônica com diferentes preparações antigênicas

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    Matos Aldo

    1997-01-01

    Full Text Available No presente trabalho, avalia-se a nova fixação de complemento, comparativamente à imunofluorescência indireta, para o diagnóstico da doença de Chagas crônica, utilizando um extrato aquoso de formas epimastigotas do Trypanosoma cruzi e três extratos etanólicos: um de epimastigotas, um de tripomastigotas e outro de amastigotas obtidas de cultura. Empregaram-se 236 amostras testadas por imunofluorescência indireta: 109 positivas (20 com diagnóstico parasitológico e 127 amostras negativas (96 de doadores de sangue e 31 de portadores de outras patologias. Os resultados mostraram que é possível obter reação positiva em amostras diluídas até 1:16. Os melhores limiares de reatividade encontrados foram a diluição 1:4 para o extrato etanólico de amastigotas e 1:2 para os demais antígenos. Os índices de correlação entre a nova fixação de complemento e imunofluorescência indireta indicaram o extrato etanólico de epimastigotas como o antígeno mais adequado para fins de diagnóstico entre as preparações testadas, tendo apresentado índice de co-positividade com a imunofluorescência indireta de 0,92207 e índice de co-negatividade de 0,90000. Conclui-se que a nova fixação de complemento mostrou-se ser uma microtécnica semi-quantitativa rápida, sensível, barata e de fácil execução, aplicável ao diagnóstico da doença de Chagas crônica.

  15. Preparação de argila modificada com cloreto de cetilpiridíneo e avaliação da interação desta com o PVC Preparation of modified clay with cetylpyridinium chloride and evaluation of their interaction with PVC

    Directory of Open Access Journals (Sweden)

    Daniel K. Resende

    2010-09-01

    Full Text Available Foi preparada uma argila modificada com cloreto de cetilpiridíneo a partir da argila sódica por troca de cátions em solução. Foi avaliada a quantidade de agente de modificação em relação à argila sódica e o tempo reacional. Os materiais obtidos foram caracterizados por difração de raio X (XRD, análise termogravimétrica (TGA e ressonância magnética nuclear (RMN de baixo campo. Após a caracterização foi confirmada a modificação da argila e, também, foi verificado que o produto obtido pode ser empregado na preparação de nanocompósitos de PVC, considerando que o início da degradação do material preparado ocorreu em temperatura superior às comumente utilizadas no processamento do polímero. A adição da argila modificada apresentou uma dispersão adequada no PVC e manteve o início da degradação do material em temperatura compatível com o processamento do polímero, gerando um nanocompósito com parte esfoliada e intercalada.A modified silicate with cetylpyridinium was prepared from sodium clay with cation exchange in solution. The amount of modification agent for clay and the reaction time were evaluated. The materials produced were characterized using X ray diffraction (XRD, termogravimetric analysis (TGA and low field nuclear magnetic resonance (NMR. The formation of new organic clay was confirmed, which was introduced in PVC for the formation of nanocomposites. The beginning of degradation of the new clay occurred at temperatures higher than commonly used in the processing of PVC. The nanocomposites were partially exfoliated and partially intercalated.

  16. Monitoramento de tempo e temperatura de distribuição de preparações à base de carne em escolas municipais de Natal (RN, Brasil Monitoring exposure time and distribution temperature of meat-based meals served in municipal schools in Natal, Brazil

    Directory of Open Access Journals (Sweden)

    Monique Silveira Rosa

    2008-02-01

    Full Text Available OBJETIVO: Monitorar o tempo e a temperatura de distribuição de preparações à base de carne servidas em escolas municipais de Natal (RN. MÉTODOS: Foram selecionadas 27 escolas da rede municipal de ensino, de forma aleatória, divididas em estratos por diferentes regiões administrativas. As medições de temperatura de preparações à base de carne foram verificadas quatro vezes em cada escola e o tempo de exposição destas foi verificado ao final da cocção e no início e no final da distribuição, com um termômetro digital do tipo espeto e relógio digital. Os resultados foram comparados com os padrões da resolução da diretoria colegiada nº 216/2004, da Agência Nacional de Vigilância Sanitária do Ministério da Saúde. RESULTADOS: Para temperatura de cocção, 100% das escolas apresentaram-se dentro dos padrões (acima de 70ºC. Entretanto, no início e no final da distribuição, 100% das escolas das Regiões Leste e Sul apresentaram temperaturas inadequadas (abaixo de 60ºC. Na Região Oeste 70% das escolas apresentaram temperaturas em desacordo no início da distribuição e 90% no final dessa etapa. Na Região Norte verificou-se que 91% das escolas apresentaram temperaturas impróprias no início e 82% no final da distribuição. As médias do tempo de espera das preparações foram de 59, 49, 66 e 48 minutos para as regiões Leste, Oeste, Norte e Sul, respectivamente. CONCLUSÃO: Há necessidade da adoção de Boas Práticas nas escolas municipais de Natal, a fim de uma manutenção efetiva da temperatura de distribuição das refeições no intuito de garantir uma alimentação de qualidade sanitária satisfatória aos escolares, evitando-se as intoxicações alimentares decorrentes da ineficácia das temperaturas.OBJECTIVE: To monitor exposure time and temperature of meat-based meals served in municipal schools in Natal, Brazil. METHODS: Twenty-seven municipal schools were randomly selected and stratified by

  17. GPR surveying of transport infrastructures and buildings; underground utility and void sensing - ongoing activities in Working Group 2 of COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Plati, Christina; Derobert, Xavier

    2015-04-01

    This work aims at presenting the ongoing research activities carried out in Working Group 2 'GPR surveying of pavements, bridges, tunnels and buildings; underground utility and void sensing' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (www.GPRadar.eu). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of Ground Penetrating Radar (GPR) techniques in civil engineering, whilst simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Four Working Groups (WGs) carry out the research activities. WG1 focuses on the development of innovative GPR equipment dedicated for civil engineering applications. WG2 deals with the development of guidelines and protocols for the surveying, through the use of a GPR system, of transport infrastructure and buildings, as well as for the sensing of utilities and voids. WG3 deals with the development of electromagnetic forward and inverse scattering methods, for the characterization of GPR scenarios, as well as with data- processing algorithms for the elaboration of the data collected during GPR surveys. WG4 is concerned with the use of GPR in fields different from the civil engineering, as well as with the integration of GPR with other non-destructive testing techniques. Each WG includes several Projects. WG2 includes five Projects. Project 2.1 focuses on outlining 'Innovative inspection procedures for effective GPR surveying of critical transport infrastructures (pavements, bridges and tunnels).' Project 2.2 is concerned with the development of 'Innovative inspection procedures for effective GPR surveying of buildings.' Project 2.3 deals with identifying 'Innovative inspection procedures for effective GPR sensing and mapping of underground utilities and voids, with a focus to urban

  18. Demographics, treatment and outcomes of atrial fibrillation in a developing country: the population-based TuRkish Atrial Fibrillation (TRAF) cohort.

    Science.gov (United States)

    Yavuz, Bünyamin; Ata, Naim; Oto, Emre; Katircioglu-Öztürk, Deniz; Aytemir, Kudret; Evranos, Banu; Koselerli, Rasim; Ertugay, Emre; Burkan, Abdulkadir; Ertugay, Emrah; Gale, Christ P; Camm, A John; Oto, Ali

    2017-05-01

    Although atrial fibrillation (AF) is increasingly common in developed countries, there is limited information regarding its demographics, co-morbidities, treatments and outcomes in the developing countries. We present the profile of the TuRkish Atrial Fibrillation (TRAF) cohort which provides real-life data about prevalence, incidence, co-morbidities, treatment, healthcare utilization and outcomes associated with AF. The TRAF cohort was extracted from MEDULA, a health insurance database linking hospitals, general practitioners, pharmacies and outpatient clinics for almost 100% of the inhabitants of the country. The cohort includes 507 136 individuals with AF between 2008 and 2012 aged >18 years who survived the first 30 days following diagnosis. Of 507 136 subjects, there were 423 109 (83.4%) with non-valvular AF and 84 027 (16.6%) with valvular AF. The prevalence was 0.80% in non-valvular AF and 0.28% in valvular AF; in 2012 the incidence of non-valvular AF (0.17%) was higher than valvular AF (0.04%). All-cause mortality was 19.19% (97 368) and 11.47% (58 161) at 1-year after diagnosis of AF. There were 35 707 (7.04%) ischaemic stroke/TIA/thromboembolism at baseline and 34 871 (6.87%) during follow-up; 11 472 (2.26%) major haemorrhages at baseline and 10 183 (2.01%) during follow-up, and 44 116 (8.69%) hospitalizations during the follow-up. The TRAF cohort is the first population-based, whole-country cohort of AF epidemiology, quality of care and outcomes. It provides a unique opportunity to study the patterns, causes and impact of treatments on the incidence and outcomes of AF in a developing country.

  19. Tu Salud ¡Sí Cuenta! Your Health Matters! A Community-wide Campaign in a Hispanic Border Community in Texas.

    Science.gov (United States)

    Heredia, Natalia I; Lee, MinJae; Mitchell-Bennett, Lisa; Reininger, Belinda M

    2017-08-14

    To evaluate a community-wide campaign, Tu Salud ¡Si Cuenta! (TSSC), in improving eating behaviors and anthropometric outcomes in Hispanic border communities. A quasi-experimental study with matched intervention and comparison communities. Cross-sectional assessments with randomly sampled adults, examined by actual exposure and site (unexposed intervention, exposed intervention, and unexposed comparison). Predominately Mexican Americans located in Brownsville, TX (intervention) and Laredo, TX (control). The TSSC campaign included television and radio segments, community health worker discussions, and newsletters delivered in Brownsville from 2005 to 2010. Healthy and unhealthy eating indices and average hip and waist circumferences. Univariable and multivariable regression models. The sample (n = 799; 400 comparison and 399 intervention) was 98% of Mexican origin; 54% had completed grade 9 or higher. Exposure to any TSSC component was associated with a lower rate of unhealthy food consumption. Compared with the unexposed intervention group, the exposed intervention for the newsletter had a higher rate of healthy eating (adjusted rate ratio = 1.18; P < .01). Compared with the unexposed intervention, the exposed intervention for the community health worker discussion had a smaller hip circumference (adjusted mean difference = -5.77 cm; P < .05) and a smaller waist circumference (adjusted mean difference = -5.25 cm; P < .05). This study provides evidence for the use of community-wide campaigns for nutrition and obesity-related outcomes in Hispanic communities. Copyright © 2017 Society for Nutrition Education and Behavior. Published by Elsevier Inc. All rights reserved.

  20. Talking about the scene of diet and cooking based on Pao Chu Tu(kitchen picture) of Han dynasty%从汉画像石庖厨图谈汉代饮食烹饪状况

    Institute of Scientific and Technical Information of China (English)

    杨金萍

    2009-01-01

    汉画像石庖厨图以生动写真的画面,再现了汉代饮食烹饪状况.山东诸城等地庖厨图,描绘了占代庞大忙碌的烹饪场面,包括宰杀、汲水、蒸煮、炙烤、酿酒等各种厨事活动,展示了汉代先进烹饪厨具如釜、甑、鼎、烤炉等,以及汉人繁复的烹饪技术如蒸、煮、炮、炙等,反映了汉代丰富的食物种类.汉画像石庖厨图为我们研究汉代饮食生活提供了珍贵的资料.%The Pao Chu Tu(kitchen picture)of Han dynasty stone relief reconstructed the state of dietary cooking of Han dynasty with vivid and authentic picture.The Pao Chu Tu(kitchen picture)of Shandong Zhucheng city depicted ancient enormous and busy cooking scene which involved all kinds of cooking activities such as butcher,bailing,stewing,roast and brewing wine etc,displaying the advanced kitchen ware e.g.kettle,caldron,tripod,oven and complex cooking technology of Han Chinese people such as steaming,boiling,stirring baking and roast erc,reflecting rich dietary categories of Han dynasty.The Pao Chu Tu (kitchen picture)of Han dynasty stone relief provide us precious datas for the purpose of the study of dictary living of Han dynasty.

  1. « El rastro de tu sangre en la nieve » : ¿ cuento de hadas sombrío o canción kitsch ?

    OpenAIRE

    Maryse Renaud

    2012-01-01

    Este artículo propone una lectura del cuento « El rastro de tu sangre en la nieve » de Gabriel García Márquez (Doce cuentos peregrinos, 1992), observando las relaciones que el mismo establece con distintas formas de la cultura popular. Considerando el contexto del llamado « realismo mágico », donde evoluciona habitualmente la producción de García Márquez, se estudian, en este relato, las múltiples relaciones inter-textuales con distintos tópicos populares, desde el cuento de hadas o de horror...

  2. Ogļhidrātu satura izvērtējums etiķskābes baktēriju fermentēšanas kultūršķidrumā

    OpenAIRE

    Beikmanis, Nils

    2015-01-01

    Darba teorētiskajā daļā tika apskatīta literatūra par iespējamo ogļhidrātu saturu fermentēšanas kultūršķidrumā un tā analīzes metodes. Praktiskajā daļā tika noteikts kvantitatīvs un kvalitatīvs kultūršķidrumu sastāvs izmantojot Lufa-Šorla metodi un augstefektīvo šķidrumu hromatogrāfiju.

  3. Professional Development of College and University English Teachers in Chang-Ji-Tu Area%长吉图区域高校外语教师专业发展研究

    Institute of Scientific and Technical Information of China (English)

    徐莹

    2014-01-01

    调查长吉图区域高校外语教师专业发展状况,对调查结果进行分析,并提出外语教师专业发展途径,以期为外语教师的专业发展提供有益的借鉴,从而提高外语教师素质,提升高校教学质量,发挥高校的优势,促进长吉图开发开放先导区建设。%This study investigates foreign language teachers' professional development in Chang-Ji-Tu area, ana-lyses the results objectively. Based on the results, constructive suggestions are made on how to enhance English teachers' professional development, aiming to improve foreign language teachers' qualities and teaching quality of colleges and universities, promote the economic construction of Chang-Ji-Tu area.

  4. Identities ofJiao, Tu and Cao from Shuihudi Bamboo Slips%从睡虎地秦简看“校、徒、操”的身份

    Institute of Scientific and Technical Information of China (English)

    仝卫敏

    2012-01-01

    Jiao [校] ,tu [徒] and cao [操] recorded in "Jing Nei Pian" [境内篇] in ShangJun Shu [商君书] are associated with the ranking system advocated by Shang Yang and their identities are controversial among scholars. By referring to Qin dynasty bamboo slips discovered at Shuihudi, Hubei province, this article points out thatjiao, tu and cao belong to a lower class in the army; instead of being handymen or sappers, they are minor officials in the army descending in rank and are not part of Shang Yang' s ranking system.%“校、徒、操”见于《商君书·境内篇》,关涉商鞅爵制等级问题,学界对其身份争议较大。本文结合睡虎地秦墓竹简的相关材料,认为“校、徒、操”尽管在军中地位较低,但并非军中杂役、苦力或工程兵,而是由高到低排列的军中小吏,且并不在商鞅爵制等级范围之内。

  5. Marijas Montesori pedagoģisko principu izmantošana sajūtu attīstībai trīs līdz četrus gadus veciem bērniem

    OpenAIRE

    Dzērve, Vita

    2012-01-01

    Tēma: Marijas Montesori pedagoģisko principu izmantošana sajūtu attīstībai trīs līdz četrus gadus veciem bērniem Kvalifikācijas darba autore: Vita Dzērve Kvalifikācijas darba vadītāja: lekt.,mag. paed., I. Šūmane Kvalifikācijas darba apjoms: 57 lpp.,16 attēli, 1 pielikums, 19 izmantoti literatūras avoti Pētījuma mērķis: izpētīt un praksē aprobēt M. Montesori pedagoģiskos principus trīs līdz četrus gadus veciem bērniem sajūtu attīstības veicināšanai. Darba pirmajā daļā ...

  6. Preparação e caracterização elétrica de sensores de metanol à base de nanocompósitos híbridos de polipirrol/nanopartículas metálicas Preparation and electrical characterization of gold nanoparticles-polypyrrole hybrid composites for application in methanol gas sensing

    Directory of Open Access Journals (Sweden)

    Helinando P. de Oliveira

    2010-01-01

    Full Text Available Neste trabalho, compósitos híbridos metal/polímero (nanopartículas de ouro/polipirrol foram sintetizados a partir da polimerização química do pirrol sobre a superfície de nanopartículas metálicas estabilizados por um agente surfactante (dodecil sulfato de sódio. A posterior solubilização do compósito em álcool polivinílico permitiu a obtenção de uma matriz orgânica auto-sustentável, flexível, com boa resistência mecânica e aplicável na detecção de compostos voláteis (com especial atenção ao metanol. Nessa direção, foram otimizadas as condições de preparação do compósito, no sentido de promover a detecção e quantificação do metanol em misturas com etanol, com o intuito de aplicar o dispositivo no reconhecimento de resquícios de metanol em bebidas alcoólicas. Dos sistemas analisados, a sensibilidade dos dispositivos se mostrou diretamente proporcional à constante dielétrica do volátil utilizado, indicando que interações físicas ocorrem na matriz na presença de voláteis.In this study, hybrid composites of gold nanoparticles and polypyrrole (AuNPs-PPy were manufactured by chemical polymerization of the pyrrole monomer on the surface of metal nanoparticles, which were then incorporated in a poly(vinyl alcohol (PVA matrix to be exploited in sensing technologies for detecting methanol. The preparation conditions were optimized to maximize sensitivity, allowing for the determination of relative concentrations of methanol molecules in binary mixtures of methanol/ethanol. This was carried out using the electrical response of the sensor, which depends on the dielectric constant of the volatile organic compounds.

  7. Preparação e caracterização microestrutural de cerâmicas ferroelétricas de Ba0,77Ca0,23TiO3 Preparation and microstructural characterization of Ba0.77Ca0.23TiO3 ferroelectric ceramics

    Directory of Open Access Journals (Sweden)

    T. Mazon

    2004-06-01

    Full Text Available Cerâmicas de titanato de bário e cálcio com composição Ba0.77Ca0.23TiO3 (BCT23 foram produzidas usando o método de síntese por reação de estado sólido. Um estudo sistemático de temperatura e tempo de calcinação foi realizado com o objetivo de se determinar as condições experimentais ótimas para a obtenção da fase única BCT23. Observou-se a presença das fases BaTiO3 e CaTiO3 para os pós calcinados em temperaturas inferiores a 1200 ºC e tempo de até 12 horas. Um aumento na temperatura para 1250 ºC leva a formação da fase BCT23. Os pós calcinados a 1250 ºC foram prensados uniaxialmente em diferentes condições e sinterizados a 1450 ºC / 4h. Verificou-se que o método utilizado para a preparação dos compactos influenciou na microestrutura e densidade relativa das cerâmicas. Cerâmicas mais densas foram obtidas a partir da prensagem com pressão uniaxial equivalente a 1x10(7 N/m² dos pós de BCT23 peneirados.Barium calcium titanate ceramics with composition Ba0,77Ca0,23TiO3 (BCT23 were prepared by solid state reaction. A systematic study of temperature and time of calcination was performed aiming to determine the experimental conditions to obtain only BCT23 phase. It was only observed the BaTiO3 and CaTiO3 phases at calcination temperature up to 1200 ºC and time up to 12 h. However, it was possible to obtain the BCT23 phase by increasing the temperature to 1250 ºC for 12 h. The powders calcined at 1250 ºC for 12 h were uniaxially pressed at different conditions and sintered at 1450 ºC / 4h. The method utilized to prepare the compacts influenced on the microstructure and relative density of the ceramics. Denser ceramics were obtained by pressing a BCT23 powder with 1x10(7 N/m².

  8. Imunodiagnóstico da leptospirose humana através do teste ELISA-IgM, empregando-se diferentes preparações antigênicas a partir de sorotipos prevalentes de Leptospira interrogans Immunodiagnostic of human leptospirosis by ELISA-IgM, employing: different antigenic preparations as from prevalent serovars of Leptospira interrogans

    Directory of Open Access Journals (Sweden)

    Marcos Vinicius da Silva

    1990-08-01

    Full Text Available Realizou-se estudo comparativo de diferentes sorotipos de Leptospira interrogans utilizados na preparação de antígenos empregados no teste ELISA, para a detecção de anticorpos da classe IgM, em amostras de soro na fase precoce e tardia da leptospirose humana. Foram utilizados dez sorotipos, escolhidos entre os que apresentaram maior reatividade na soroaglutinação microscópica (SAM, na cidade de São Paulo. Os cinco sorotipos que apresentaram melhores resultados individualmente no teste ELISA-IgM (canicola, hebdomadis, icterohaemorrhagiae, cynopteri e brasiliensis, foram também estudados em mistura antigênica. Os antígenos não tratados apresentaram maior reatividade do que os antígenos tratados com Triton X - 100 (4% à temperatura de 50ºC, durante 4 horas. O teste ELISA-IgM empregando os sorotipos não tratados, isoladamente, e em mistura antigênica, mostrou-se altamente sensível, podendo ser empregado como teste de triagem para o diagnóstico precoce da leptospirose humana. Outra aplicação do teste é permitir a detecção do início de situações epidêmicas ou de surtos, possibilitando acionar medidas de vigilância epidemiológica.A comparative study among different serovars of Leptospira interrogans was performed in order to prepare antigens to detect IgM antibodies by ELISA in early and late phase of human leptospirosis. Ten serovars were chosen among the most prevalent detected by microscopic seroagglutination (SAM in São Paulo city. Using ELISA-IgM five of them showed better results (canicola, hebdomadis, icterohaemorrhagiae, cynopteri and brasiliensis. These ones were also studied in a pool. The non-treated antigens showed higher reactivity than the Triton X-100 (4%/50ºC/4h. ELISA-IgM using individually or pool of non treated antigens proved to be reliable with high sensitivity and should be used for an earlier diagnosis of leptospirosis, as a trial test. Faster diagnostic elucidation can be useful to detect

  9. Aquecimento de vinho tinto e suco de uva utilizados em preparações culinárias não afeta a capacidade antioxidante e o teor de fenóis totais Heating of red wine and grape juice in food preparations does not affect their antioxidant capacity and total phenol content

    Directory of Open Access Journals (Sweden)

    Jeanine Schütz Cardoso Teófilo

    2011-02-01

    Full Text Available OBJETIVO: Avaliar a capacidade antioxidante e a concentração dos compostos fenólicos de vinho tinto e suco de uva utilizados em preparações culinárias, quando submetidos ao processo de fervura. Métodos: A avaliação ocorreu após diferentes períodos de aquecimento (100°C em dois sistemas: i panela de alumínio e chama de fogão e ii balão de vidro e manta térmica. Amostras comerciais de vinho tinto (n=4 e suco de uva integral (n=4 foram submetidas à fervura por 10, 20 e 60 minutos. Antes e após cada período de fervura, a capacidade antioxidante total foi avaliada pela medida do potencial redutor férrico, e o teor de fenóis totais foi quantificado pelo método de Folin-Ciocalteau. As diferenças foram comparadas pela análise de variância para medidas repetidas (pObjective: This study evaluated the antioxidant capacity and concentration of phenolic compounds in red wine and grape juice used in culinary preparations subjected to boiling. Methods: The assessments were done after different boiling times (100°C using two systems: i aluminum pot and stove flame and ii volumetric flask and heating mantle. Samples of red wine (n=4 and grape juice (n=4 were subjected to boiling for 10, 20 and 60 minutes. Before and after each boiling period, the total antioxidant capacity was evaluated by the ferric reducing antioxidant power assay and the total phenol content was determined by the Folin-Ciocalteu reagent. Differences were compared by repeated measures analysis of variance (RM-ANOVA (p<0.05. Results: The different boiling times did not cause significant changes in the total antioxidant capacity or total phenol content of the beverages, regardless of boiling method. Conclusion: The results show that the antioxidant properties of red wine and grape juice did not change significantly after boiling for 60 minutes.

  10. Efeitos das condições de preparação e de modificação de superfície de membranas de PEUAPM na separação água/óleo Preparation and surface modification effects of UHMWPE membranes for oil/water separation

    Directory of Open Access Journals (Sweden)

    Laura H. de Carvalho

    2009-01-01

    Full Text Available Baixos custos operacionais, operação relativamente simples, scale-up e manutenção são as principais razões para uso de membrana no processo de separação numa variedade ampla de aplicações, incluindo purificação de efluentes aquosos. Neste trabalho, a influência dos parâmetros de processo na preparação de membranas de PEUAPM simétricas e assimétricas foi investigada. Membranas de PEUAPM tubulares simétricas foram preparadas com pó passante em peneiras de malha # 80 e # 100. As condições de processamento foram: a temperatura (200 e 220 °C; b pressões de compactação (0, 4 e 6 kgf/cm²; e c tempo de sinterização (90 minutos. Membranas assimétricas foram obtidas por deposição de um filme de PEAD sobre a superfície interna das membranas simétricas selecionadas. As Membranas foram caracterizadas por MEV, porosimetria de mercúrio e medidas de fluxo. Nossos dados indicam que, para membranas simétricas, a porosidade e fluxo permeado diminuíram com o aumento da temperatura e pressões de compactação. Redução mais acentuada nestas variáveis foi observada com membranas assimétricas.Low operational costs, relatively simple operation, scale-up and maintenance are the main reason for using membrane separation processes in a wide range of applications. The purpose of this work was to prepare, characterize and investigate the influence of processing parameters (T, P and of an internal surface modification on the performance of UHMWPE membranes. Sinterization was the technique used for membrane preparation. Tubular sintered UHMWPE membranes were prepared with powders sieved through #80 and #100 mesh. Processing conditions were: a temperature (200 and 220 °C; b compacting pressures (0, 4 and 6 kgf/cm²; and c sintering time (90 minutes. Surface modified membranes were obtained by internally impregnating selected UHMWPE membranes with a HDPE dilute solution. Membranes were characterized by SEM, mercury porosimetry water

  11. Quantitative determination of ciprofloxacin and norfloxacin in pharmaceutical preparations by high performance liquid chromatography Determinação quantitativa do ciprofloxacino e do norfloxacino em preparações farmacêuticas por cromatografia líquida de alta eficiência

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    Nájla Mohamad Kassab

    2005-12-01

    Full Text Available The objective of this research was to develop and validate an analytical method for quantitative determination of ciprofloxacin (CIP and norfloxacin (NOR in pharmaceutical preparations. A simple and rapid chromatographic method was developed and validated for quantitative determination of two fluoroquinolone antibiotics in tablets and injection preparations. The quinolones were analyzed by using a LiChrospher® 100 RP-18 column (5 µm, 125 x 4 mm and a mobile phase consisted of water:acetonitrile:triethylamine (80:20:0.3 v/v/v. The pH of final mixture was adjusted to 3.3 with phosphoric acid. The flow rate was 1.0 mL/min and UV detection was made at 279 nm. The analyses were performed at room temperature (24 ± 2 ºC. CIP and NOR were eluted within 5 min. The calibration curves were linear (r > 0.9999 over a concentration range from 4.0 to 24.0 µg/mL. The relative standard deviation (RSD was O objetivo desta pesquisa foi desenvolver e validar um método analítico para a determinação quantitativa do ciprofloxacino (CIP e do norfloxacino (NOR em preparações farmacêuticas. Um método cromatográfico simples e rápido foi desenvolvido e validado para a determinação quantitativa de dois antibióticos fluoroquinolônicos em comprimidos e injetáveis. As quinolonas foram analisadas empregando-se uma coluna RP-18, LiChrospher® 100 (125 x 4 mm, 5µm e fase móvel constituída por água:acetonitrila:trietilamina (80:20:0.3 v/v/v. O pH da mistura final foi ajustado a 3,3 com ácido fosfórico. A vazão da fase móvel foi de 1,0 mL/min e a detecção em UV a 279 nm. As análises foram realizadas à temperatura ambiente (24±2 ºC. O CIP e o NOR eluíram em menos de 5 min. As curvas de calibração foram lineares (r > 0.9999 na faixa de concentração entre 4,0 e 24,0 µg/mL. O desvio padrão relativo (RSD foi < 1,0% e a recuperação média foi de 101,85%.

  12. Revisão sistemática sobre antissepsia cirúrgica das mãos com preparação alcoólica em comparação aos produtos tradicionais Revisión sistemática sobre antisepsia quirúrgica de manos con preparación alcohólica comparada a productos tradicionales A systematic review of surgical hand antisepsis utilizing an alcohol preparation compared to traditional products

    Directory of Open Access Journals (Sweden)

    Karen de Jesus Gonçalves

    2012-12-01

    Full Text Available A antissepsia cirúrgica das mãos visa à prevenção de infecções do sítio cirúrgico, importante causa de morbimortalidade pós-operatória e aumento dos custos hospitalares. Este estudo teve como objetivo comparar a eficácia de preparações alcoólicas com os produtos tradicionais na antissepsia cirúrgica das mãos por meio de uma revisão sistemática da literatura. Foram considerados estudos primários ou secundários, tendo como desfecho a contagem microbiana das mãos ou taxas de infecções do sítio cirúrgico. A busca foi realizada no Portal BVS, PubMed, Ask e MEDLINE. Foram selecionados 25 estudos (2 revisões sistemáticas, 19 experimentais e 4 de coorte. As preparações alcoólicas tiveram uma redução microbiana igual e/ou maior aos produtos tradicionais em 17 estudos e inferior em 4; as taxas de infecções do sítio cirúrgico foram similares. Portanto, existem evidências científicas que suportam a segurança das preparações alcoólicas para antissepsia cirúrgica das mãos.La antisepsia quirúrgica de manos apunta a prevenir infecciones en el sitio quirúrgico, causa importante de morbi-mortalidad postoperatoria y aumento de costos hospitalarios. El estudio objetivó comparar la eficacia de preparaciones alcohólicas con los productos tradicionales de la antisepsia quirúrgica de manos, mediante revisión sistemática de la literatura. Fueron considerados estudios primarios o secundarios, teniendo como objetivo el recuento microbiano en manos o tasas de infecciones del sitio quirúrgico. La búsqueda fue realizada en las bases BVS, PubMed, Ask y MEDLINE. Fueron seleccionados 25 estudios (2 revisiones sistemáticas, 19 experimentales y 4 de cohorte. Las preparaciones alcohólicas consiguieron una reducción microbiana igual y/o mayor que los productos tradicionales en 17 estudios, e inferior en 4; las tasas de infección del sitio quirúrgico fueron equivalentes. Por lo tanto, existen evidencias científicas que dan

  13. Design and testing of Ground Penetrating Radar equipment dedicated for civil engineering applications: ongoing activities in Working Group 1 of COST Action TU1208

    Science.gov (United States)

    Pajewski, Lara; Manacorda, Guido; Persico, Raffaele

    2015-04-01

    This work aims at presenting the ongoing research activities carried out in Working Group 1 'Novel GPR instrumentation' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (www.GPRadar.eu). The principal goal of the COST Action TU1208 is to exchange and increase scientific-technical knowledge and experience of GPR techniques in civil engineering, simultaneously promoting throughout Europe the effective use of this safe and non-destructive technique in the monitoring of infrastructures and structures. Working Group 1 (WG1) of the Action focuses on the development of innovative GPR equipment dedicated for civil engineering applications. It includes three Projects. Project 1.1 is focused on the 'Design, realisation and optimisation of innovative GPR equipment for the monitoring of critical transport infrastructures and buildings, and for the sensing of underground utilities and voids.' Project 1.2 is concerned with the 'Development and definition of advanced testing, calibration and stability procedures and protocols, for GPR equipment.' Project 1.3 deals with the 'Design, modelling and optimisation of GPR antennas.' During the first year of the Action, WG1 Members coordinated between themselves to address the state of the art and open problems in the scientific fields identified by the above-mentioned Projects [1, 2]. In carrying our this work, the WG1 strongly benefited from the participation of IDS Ingegneria dei Sistemi, one of the biggest GPR manufacturers, as well as from the contribution of external experts as David J. Daniels and Erica Utsi, sharing with the Action Members their wide experience on GPR technology and methodology (First General Meeting, July 2013). The synergy with WG2 and WG4 of the Action was useful for a deep understanding of the problems, merits and limits of available GPR equipment, as well as to discuss how to quantify the reliability of GPR results. An

  14. Izlaganje na javni uvid podataka o nekretninama i utvrđivanje prava na zemljištu u funkciji uspostave jedinstvenog registra zemljišta i prava na zemljištu u Brčko distriktu BiH : Public presentation of data on real-estate and determination of rights in function of the establishment of a single registry of real-estate and real rights to real-estate in the Brcko district of B&H

    Directory of Open Access Journals (Sweden)

    Faruk Latifović

    2014-12-01

    Full Text Available Zakon o registru zemljišta i prava na zemljištu Brčko distrikta Bosne i Hercegovine je propisao uspostavu jedinstvene evidencije nekretnina i stvarnih prava na nekretninama u Brčko distriktu Bosne i Hercegovine. Poslovi uspostave jedinstvene evidencije, u segmentu izlaganja na javni uvid podataka o nekretninama i utvrđivanje prava, su povjereni Vijeću za izlaganje podataka o nekretninama i utvrđivanje prava na zemljištu Brčko distrikta Bosne i Hercegovine. Autor se, u ovom radu, uz nezaobilazan kraći prikaz historijata javnih evidencija na nekretninama u Bosni i Hercegovini, bavi organizacijom, nadležnostima i načinom rada Vijeća za izlaganje podataka o nekretninama i utvrđivanje prava na zemljištu Brčko distrikta BIH. Nakon što bude uspješno okončan projekat izlaganje podataka o nekretninama i utvrđivanje prava na zemljištu u Brčko distriktu BiH, svi geodetsko – katastarski podaci o nepokretnostima kao i podaci o njihovom pravnom statusu će biti vođeni u Registru zemljišta i prava na zemljištu Osnovnog suda Brčko distrikta BiH. : The Law of Land Registry and Rights over Land of the Brčko District of B&H stipulated the establishment of a single registry of real-estate and real rights to real-estate in the Brčko District of B&H. Activities of the establishment of a single registry, in the segment of public presentation of data on real-estate and determination of rights, were entrusted to the Council for Presentation of Data on Real-estate and Establishment of Rights to Land of the Brčko District of B&H. In this paper, the author presents the inevitable short overview of the history of real-estate registries in Bosnia and Herzegovina and he also deals with organization, competencies and manner of work of the Council for Presentation of Data on Real-estate and Establishment of Rights to Land of the Brčko District of B&H. After a successful completion of the project of presentation of data on real-estate and

  15. TU Delft en online media

    NARCIS (Netherlands)

    Speekenbrink, R.J.H.

    2013-01-01

    Dit boek behandelt een visie op online media bij Nederlandse universiteiten. Daarbij beperk ik me tot die aspecten waarin online communicatie en/of online marketing een rol speelt. Dit boek behandelt niet de onderwijsgerelateerde aspecten van online media. Elektronische leeromgevingen, sociale medi

  16. TU Delft en online media

    NARCIS (Netherlands)

    Speekenbrink, R.J.H.

    2013-01-01

    Dit boek behandelt een visie op online media bij Nederlandse universiteiten. Daarbij beperk ik me tot die aspecten waarin online communicatie en/of online marketing een rol speelt. Dit boek behandelt niet de onderwijsgerelateerde aspecten van online media. Elektronische leeromgevingen, sociale medi

  17. TU Delft en online media

    NARCIS (Netherlands)

    Speekenbrink, R.J.H.

    2013-01-01

    Dit boek behandelt een visie op online media bij Nederlandse universiteiten. Daarbij beperk ik me tot die aspecten waarin online communicatie en/of online marketing een rol speelt. Dit boek behandelt niet de onderwijsgerelateerde aspecten van online media. Elektronische leeromgevingen, sociale

  18. Preparação da mulher para a realização do exame de Papanicolaou na perspectiva da qualidade Preparación de la mujer para la realización del examen de Papanicolaou en la perspectiva de la calidad Woman's preparation for the Papanicolaou's exam in the perspective of the quality

    Directory of Open Access Journals (Sweden)

    Kylvia Gradênia Torres Eduardo

    2007-03-01

    Full Text Available OBJETIVO: avaliar a preparação da mulher para a realização do exame de Papanicolaou. MÉTODOS: os dados foram coletados por meio da observação direta, seguindo o que preconiza o Instrumento de Melhoria de Desempenho (IMD, durante o mês de setembro de 2005. Participaram os sete enfermeiros do Programa Saúde da Família de um município cearense, os quais tiveram suas práticas observadas nos respectivos sítios de atuação (Unidade Básica de Saúde. Cada enfermeiro foi acompanhado em três exames, totalizando 21 observações. RESULTADOS: dentre as 21 observações realizadas, em nenhuma, os enfermeiros endagaram se a usuária havia esvaziado a bexiga; em 8 (38,1% estas profissionais explicaram sobre o exame; e, em 18 (85,7%, respectivamente orientaram para a troca de roupa em local privativo e cobririam a usuária de forma adequada durante o exame. CONCLUSÕES: a preparação das mulheres mostrou-se insatisfatória, pois os quatro itens preconizados no IMD só foram cumpridos em 52,4% das vezes. Transformar esta realidade exige mudanças de atitude humana, área da competência técnica de maior desafio no contexto do serviço público.OBJETIVO: evaluar la preparación de la mujer para la realización del examen de Papanicolaou. MÉTODOS: los datos fueron recolectados por medio de la observación directa, siguiendo lo que preconiza el Instrumento de Mejoría del Desempeño (IMD, durante el mes de setiembre del 2005. Participaron los siete enfermeros del Programa Salud de la Familia de un municipio cearense, los fueron cuales tuvieron observados durante la realización de sus prácticas clínicas en los respectivos locales de actuación (Unidad Básica de Salud. Cada enfermero fue acompañado en tres exámenes, con un total de 21 observaciones. RESULTADOS: de las 21 observaciones realizadas, en ninguna de ellas los enfermeros preguntaron si la usuaria habia orinado; en 8 (38,1% estas profisionales dieron explicaciones sobre el examen y

  19. Dietas enterais não industrializadas: análise microbiológica e verificação de boas práticas de preparação Non industrialized enteral diets: microbiological analysis and verification of good preparation practices

    Directory of Open Access Journals (Sweden)

    Angélica Aparecida Maurício

    2008-02-01

    Full Text Available OBJETIVO: Verificar a adequação das áreas de produção e o nível de contaminação microbiana de dietas enterais utilizadas em três hospitais privados da Região Noroeste do Paraná. MÉTODOS: A análise das Boas Práticas de Preparação da Nutrição Enteral seguiu a Resolução da Diretoria Colegiada nº 63/2000, da Agência Nacional de Vigilância Sanitária, e as análises microbiológicas das 15 amostras de dietas enterais seguiram as recomendações da Resolução da Diretoria Colegiada nº 12/2001, da Agência Nacional de Vigilância Sanitária, nas quais foram pesquisados bolores e leveduras, bactérias mesófilas, coliformes a 35 e 45ºC, Estafilococos coagulase positiva, Salmonella sp. e Bacillus cereus. RESULTADOS: A maior porcentagem para as Não Conformidades foi do hospital HS (61% e a menor do hospital HT (43,5%. Foi observada, em 100% das amostras dos hospitais HP e HT, contaminação por coliformes a 45ºC acima do padrão de referência da Resolução da Diretoria Colegiada nº 12/2001 e 60% das amostras do hospital HS apresentaram contaminação por coliformes a 35ºC acima do limite aceitável. As amostras encontravam-se dentro dos padrões para Estafilococos coagulase positiva, Salmonella sp., Bacillus cereus e bactérias mesófilas. Nos hospitais HT e HP a contaminação por bolores e leveduras estava acima do padrão permitido pela legislação. Os resultados das análises mostraram que a contaminação das dietas está relacionada ao tipo de ingrediente utilizado e ao excesso de manipulação. CONCLUSÃO: Conclui-se que as boas práticas de preparação da nutrição enteral não estavam de acordo com a Resolução da Diretoria Colegiada nº 63/2000 e que os hospitais estavam inadequados para preparar esse tipo de alimento.OBJECTIVE: To verify the degree of suitability of production areas and the level of microbial contamination in enteral diets used in three private hospitals in the Northwest region of Paran

  20. Should Degree Programs in Biomedical and Health Informatics be Dedicated or Integrated? : Reflections and Recommendations after more than 40 Years of Medical Informatics Education at TU Braunschweig, including 10 Years of B.Sc. and 15 Years of M.Sc. Integrated Degree Curricula.

    Science.gov (United States)

    Haux, Reinhold; Marschollek, Michael; Wolf, Klaus-Hendrik; Zeisberg, Ute

    2017-07-01

    Education in biomedical and health informatics (BMHI) has been established in many countries throughout the world. For degree programs in BMHI we can distinguish between those that are completely stand-alone or dedicated to the discipline vs. those that are integrated within another program. After running integrated degree medical informatics programs at TU Braunschweig for 10 years at the B.Sc. and for 15 years at the M.Sc level, we (1) report about this educational approach, (2) analyze recommendations on, implementations of, and experiences with degree educational programs in BMHI worldwide, (3) summarize our lessons learned with the integrated approach at TU Braunschweig, and (4) suggest an answer to the question, whether degree programs in biomedical and health informatics should be dedicated or integrated. According to our experience at TU Braunschweig and based on our analysis of publications, there is a clear dominance of dedicated degree programs in BMHI. The specialization in medical informatics within a computer science program, as offered at TU Braunschweig, may be a good way of implementing an integrated, informatics-based approach to medical informatics, in particular if a dual degree option can be chosen. The option of curricula leading to double degrees, i.e. in this case to two separate degrees in computer science and in medical informatics might, however, be a better solution.

  1. Ar fotosintēzi saistīto parametru sezonālās izmaiņas dažādām boreonemorālo briofītu sugām atkarībā no ūdens satura

    OpenAIRE

    Gaile, Laura

    2014-01-01

    Darbā aprakstītas ar fotosintēzi saistīto parametru izmaiņas dažādām briofītu sugām boreonemorālajos biotopos saistībā ar ūdens saturu. Mērķis bija noteikt fotosintēzes pigmentu koncentrāciju sezonālās izmaiņas dažādām briofītu sugām no atšķirīgiem biotopiem un meklēt to saistību ar augšanas substrātu, funkcionālo piederību, ūdens saturu, kā arī, fotosintēzes fotoķīmisko reakciju intensitāti, ko raksturo hlorofila fluorescences rādītāji. Kopā ievāca 25 briofītu sugu 26 paraugus no pieciem daž...

  2. Tentativas de preparação de alcaloides azahomoaporfinicos

    OpenAIRE

    Genaro Carrasco Leiva

    1991-01-01

    Resumo: Recentemente foram isolados novos alcalóides da classe azahomoaporfínicos. Estes alcalóides, que possuem sistemas piridínicos e benzazepínicos fundidos, nos levou a investigar seu potencial sintético. A proposta geral biogenética envolve a fissura do anel seguido depois por fechamento, que pode dirigir à formação de um heterocíclico contendo um átomo de nitrogênio. Sendo este, o primeiro exemplo de aporfínicos incorporando um segundo átomo de nitrogênio em anel C. Tentamos uma rota si...

  3. Analise quantitativa de diclofenaco em preparações farmaceuticas

    OpenAIRE

    2004-01-01

    Resumo: O diclofenaco é um composto não-esteróide com propriedades anti-reumáticas, antiinflamatórias e antipiréticas. Ele é comercializado na forma de sal de sódio e de potássio. Há na literatura um número significativo de procedimentos analíticos para a quantificação deste princípio ativo, contudo, a maioria destas técnicas é cara e de procedimento relativamente demorado. Existem poucos procedimentos experimentais na região do visível para a sua análise quantitativa. Neste trabalho foram es...

  4. The Ever Flowing River Witness the History---TU Fu’ s Poetry and his poetic life%不废江河万古流--杜甫的诗歌与诗性人生

    Institute of Scientific and Technical Information of China (English)

    刘国庚

    2015-01-01

    唐代由盛及衰的离乱之世恰是中国诗坛群星璀璨的繁华时代。其中成就最为卓著、最具代表性的诗人,是“诗圣”杜甫。杜甫诗歌沉郁顿挫、兼容并蓄,其恒久魅力在于强烈的思想穿透力、人格亲和力以及诗中蕴藉的忧国忧民、悲天悯人的情怀和执著弘毅、挫而不折的品质。杜甫用一生的坚守和求索,筑起中国文学史上的一座圣殿,获得了崇高的历史地位。1200多年来,杜甫的诗风与人品,相映生辉,相得益彰,一直被知识阶层当作情感凭藉和行为楷模。%The periods of historic transformation in Tang dynasty shifting from prosperity to ruin happened to be the prosperous times of the famous and numerous composers of Chinese poetry.And among them the most distinguished and representative poet was Master Poet,Tu Fu.Tu Fu’ s poems were depressed,sonorous,and inclusive,and the e-ternal charm lied in his strong mental penetrating power and his character of affinity as well as his serious concern for his country,his people and his personality of perseverance embodied in his poems.With perseverant exploration throughout his life,he built up a great temple in the history of Chinese literature and achieved a lofty historical posi-tion.As Tu Fu’ s poetic style and moral quality complemented each other so much that he has long been taken as both emotional solace and role model for over 1 200 years.

  5. Starppersonu komunikācijas veida saistība ar pusaudžu pašvērtējumu un vientulības izjūtu

    OpenAIRE

    Kreitūze, Ieva

    2016-01-01

    Pētījuma mērķis ir noskaidrot vai pusaudžu komunikācija ir saistīta ar pusaudžu pašvērtējumu un vientulības izjūtu. Pētījuma mērķa sasniegšanai tika izmantota Rozenberga pašvērtējuma skala, (Rosenberg Self-Esteem Scale, Rosenberg, 1965), UCLA vientulības skala, (The Revised UCLA Loneliness Scale, Russell, Peplau, & Cutrona, 1996). Komunikācijas izpētei tika izmantota Interpersonālās komunikācijas skala, (Interpersonal Communication Inventory, Millard & Bienvenu, 1971 Interpersonal Communicat...

  6. Review: Hu Houxuan, "Xia and the Tu State in the Oracle Inscriptions: A Consideration" Gudai Chengbang Shi Yanjiu (Studies on Ancient City States) Renmin Press, 1989, Pp. 340-353.

    Institute of Scientific and Technical Information of China (English)

    RiZhi

    1995-01-01

    This article verified that the Tu state mentioned in the oracle inscriptions was the Xia people after the Xia Dynasty had been conquered by Tang, the first king of the Shang Dynasty m c.a. 16th century B.C. The state of Xia, which originated from Yu, was entitled "Xia Hou"; its king was hence called "Xia Hou Di" (Emperor of Xia Hou), e.g."Xia Hou Di Qi". The Xia people, together with the Yin (Shang) people, and the Zhou people, were the so-called "people of San Dai" (Three Dynasties). As mentioned in "Lunyu, Bayi", they founded the states, with the Xia people being associated with the pine, the Yin people with the cypress, and the Zhou people with the chestnut.

  7. Starppersonu komunikācijas veida saistība ar pusaudžu pašvērtējumu un vientulības izjūtu

    OpenAIRE

    Kreitūze, Ieva

    2016-01-01

    Pētījuma mērķis ir noskaidrot vai pusaudžu komunikācija ir saistīta ar pusaudžu pašvērtējumu un vientulības izjūtu. Pētījuma mērķa sasniegšanai tika izmantota Rozenberga pašvērtējuma skala, (Rosenberg Self-Esteem Scale, Rosenberg, 1965), UCLA vientulības skala, (The Revised UCLA Loneliness Scale, Russell, Peplau, & Cutrona, 1996). Komunikācijas izpētei tika izmantota Interpersonālās komunikācijas skala, (Interpersonal Communication Inventory, Millard & Bienvenu, 1971 Interpersonal Communicat...

  8. Gallija-68 [68Ga] un fluora-18 [18F] izotopu iegūšana un izmantošana radiofarmaceitisko preparātu sintēzē

    OpenAIRE

    Mamis, Edgars

    2017-01-01

    Gallija-68 [68Ga] un fluora-18 [18F] izotopu iegūšana un izmantošana radiofarmaceitisko preparātu sintēzē. Mamis E., zinātniskais vadītājs Prof. Dr.h.c. Chem Andris Actiņš, zinātniskie konsultanti Mg. Chem Jāzeps Malnačs un Mg. Med. Phys., Dipl. Ing., Mg. Sc. Ing Linda Meistere. Maģistra darbs, 54 lappuses, 28 attēli, 12 tabulas, 20 literatūras avoti. Latviešu valodā. Darbā ir apkopota literatūra par radionuklīdu iegūšanas procesiem kodolreakcijas ceļā, izmantojot daļiņu paātrinātāju – ciklo...

  9. Validação de método para avaliação da qualidade sanitária de preparação cosmética de base lipófila Validation of method to evaluate sanitary quality of lipophilic base cosmetics

    Directory of Open Access Journals (Sweden)

    Nádia Araci Bou-Chacra

    2003-06-01

    Full Text Available A validação de método para a avaliação da qualidade sanitária de produto cosmético de base lipófila foi efetuada empregando-se neutralizantes químicos e diluição para a eliminação de efeito inibitório no crescimento microbiano. Para a preparação da amostra foi empregado miristato de isopropila e polissorbato 20. Além dessas substâncias, foi adicionado ao diluente 0,5% (p/v de lecitina de soja visando à neutralização do cloreto de benzalcônio. Os ensaios foram efetuados objetivando a comprovação da ausência de efeito inibitório no crescimento microbiano decorrente de atividade antimicrobiana do produto, assim como dos agentes neutralizantes, tensoativos e solubilizante empregados no ensaio. Na avaliação quantitativa, os resultados demonstraram ausência de efeito inibitório no crescimento microbiano nas diluições 1:10 e 1:100. A porcentagem de microrganismos recuperados foi de, no mínimo, 93,2% indicando a validade do método de acordo com o critério oficial adotado. Com referência ao ensaio qualitativo, foi observado efeito inibitório na diluição 1:20 para os seguintes microrganismos: Pseudomonas aeruginosaeStaphylococcus aureus.Esse efeito foi eliminado empregando-se diluição 1:200, sendo, desta forma, essa diluição considerada válida para o ensaio.The validation of method to evaluate the sanitary quality of lipophilic base cosmetics was performed using chemical neutralizers and dilution in order to eliminate the inhibitory effect on the microbial growth. The preparation of the sample was performed aiming the homogeneity in the culture media. Isopropyl miristate was used for the solubilization of the sample and polysorbate 20 was used as surfactant. Besides these substances, it was added to the diluent 0.5% (w/v of soy lecithin to neutralize the antimicrobial activity of the benzalkonium chloride. The purpose of the assays was to demonstrate the lack of inhibitory effect on the microbial growth due to

  10. Conocimiento de la ley y preparación del personal sanitario sobre el proceso asistencial de la muerte Conhecimento em legislação e preparação dos profissionais de saúde sobre o processo de cuidado da morte Legislative knowledge and preparation of health personnel for the care process of death

    Directory of Open Access Journals (Sweden)

    Luisa María Torres-Mesa

    2013-04-01

    Full Text Available Este estudio apunta a conocer la percepción que los profesionales de la salud (médicos, enfermeras y auxiliares de enfermería tienen acerca de su preparación en el cuidado de los enfermos terminales y determinar sus conocimientos sobre la legislación de cuidados paliativos. Se ha realizado un estudio transversal, en un hospital de Granada (España, mediante la aplicación de un cuestionario ad hoc. Los resultados muestran que la mayoría del personal ha trabajado con pacientes en el final de su vida, pero sólo la mitad cree tener la formación adecuada para cuidarlos. Una parte considerable dice no conocer la legislación actual en cuidados paliativos. La mayoría de los profesionales plantearía la retirada de terapias para el mantenimiento de la vida y desconocen el mecanismo para informar sobre la cumplimentación del Testamento Vital, como indica el Plan de Cuidados Paliativos de Andalucía (España.Os objetivos deste estudo são determinar a percepção que os profissionais de saúde (médicos, enfermeiros e auxiliares de enfermagem têm sobre a sua preparação sobre o cuidado dos doentes terminais e determinar seu conhecimento da legislação sobre cuidados paliativos. Realizou-se um estudo transversal em um hospital em Granada (Espanha, através da aplicação de um questionário ad hoc. Os resultados mostram que a maioria do pessoal trabalha com pacientes no fim de sua vida, mas apenas a metade crê que têm o treinamento adequado para cuidar deles. Uma proporção significativa disse que não conhece a legislação vigente sobre cuidados paliativos. A maioria dos profissionais propenderia a retirada de terapias para a sustentação da vida e não sabem o mecanismo de comunicação sobre a realização do Living Will, como indica o Plano de Assistência Paliativa de Andaluzia (Espanha.The aims of this study were to ascertain the perception that health professionals (doctors, nurses and nursing assistants have about their

  11. Preparação e caracterização de materiais híbridos celulose/NbOPO4.nH2O a partir de celulose branqueada de bagaço de cana-de-açúcar Preparation and characterization of a cellulose/NbOPO4.nH2O hybrid from bleached sugarcane bagasse

    Directory of Open Access Journals (Sweden)

    Paulo H. F. Pereira

    2012-01-01

    Full Text Available Este trabalho descreve a preparação e caracterização do híbrido celulose/fosfato de nióbio hidratado (Cel/NbOPO4.nH2O utilizando como material de partida a celulose branqueada de bagaço de cana-de-açúcar e o nióbio metálico. O híbrido foi preparado por dois métodos: denominados métodos I e II. O método I consistiu na dissolução do nióbio metálico em solução de ácido fluorídrico/nítrico (100:10, com tempo de contato de 16 horas entre a celulose branqueada do bagaço de cana-de-açúcar e a solução ácida. Adicionou-se a essa solução ácido fosfórico para precipitação do material. O método II foi semelhante, com proporções diferentes da solução ácida fluorídrico/nítrico (160/100, sem tempo de contato da celulose com a solução ácida. Os métodos I e II utilizaram as seguintes proporções de cel/ Nb2O5.nH2O: (10:10;(10:6 e (10:2. Os materiais preparados pelos métodos I e II foram caracterizados por difração de raios X (DRX, termogravimetria (TG/DTG e microscopia eletrônica de varredura (MEV. Observou-se aumento do índice do grau de cristalinidade e mudanças na morfologia dos materiais preparados.This work describes the preparation and characterization of cellulose/hydrous niobium phosphate hybrid with bleached cellulose from sugarcane bagasse. The hybrid was prepared in two different forms: named methods I and II. Method I consisted in dissolving metallic niobium in a fluoridric/nitric (100:10 mixture, with aging time of 16 hours in which the sugarcane bagasse was in contact with the acid solution. Phosphoric acid was then added to the solution for precipitation of the material. Method II was similar, but metallic niobium was diluted in a fluoridric /nitric (160/100 solution without aging time. In both methods different proportions of Cel/NbOPO4.nH2O were used: (10:10; (10:6 and (10:2. These materials were characterized by X-ray diffraction (XRD, thermogravimetry (TG/DTG analysis and scanning

  12. Preparação de um material de referência certificado para controle de agrotóxicos em hortifrutigranjeiros: estudo da homogeneidade Preparation of a certified reference material for pesticide control in the cultivation of fruits and vegetables: a homogeneity study

    Directory of Open Access Journals (Sweden)

    Maria Helena Wohlers Morelli Cardoso

    2010-06-01

    Full Text Available Neste trabalho são apresentados os esforços para garantir a homogeneidade de um material de referência. Níveis residuais (mg.kg-1 de quatro agrotóxicos (γ-HCH, fenitrotiona, clorpirifós e procimidona foram adicionados à polpa de tomate com o objetivo de se preparar um material de referência certificado. As propriedades mais importantes desses materiais são a homogeneidade e a estabilidade. Antes de serem enviados a outros laboratórios, os materiais de referência precisam ter sua homogeneidade verificada. Nas etapas prévias, amostras foram avaliadas de modo a prover dados sobre o tratamento mais adequado para minimizar a variabilidade analítica do lote preparado e garantir a qualidade da amostra candidata a material de referência certificado, tão bem como a estimativa da incerteza associada à homogeneidade. A análise de variância fornece informações sobre a variabilidade do lote preparado e o grau de invariabilidade da amostra fortificada. Depois da preparação, as amostras foram expostas à radiação gama na dose de 2 kGy e submetidas a um estudo interlaboratorial para certificação. As concentrações certificadas dos agrotóxicos após caracterização foram 0,191 ± 0,047 mg.kg-1; 0,192 ± 0,068 mg.kg-1; 0,225 ± 0,076 mg.kg-1e 0,177 ± 0,051 mg.kg-1 para o γ-HCH, a fenitrotiona, clorpirifós e procimidona respectivamente.This study reports on the efforts to ensure homogeneity of certified reference materials. Residues of four pesticides (γ-HCH, fenitrothion, chlorpyrifos, and procymidone were added to tomato pulp in order to produce reference materials. The two most important properties of such materials are known to be homogeneity and stability. Before being shipped to other laboratories, reference materials must have their homogeneity certified. As part of a preliminary study, hundreds of samples were investigated in order to provide data on the most suitable treatments to minimize analytical variability and

  13. Enantiomeric separation and quantitative determination of propranolol enantiomers in pharmaceutical preparations by chiral liquid chromatography Separação e determinação quantitativa dos enantiômeros do propranolol em preparações farmacêuticas por cromatografia quiral

    Directory of Open Access Journals (Sweden)

    Anil K. Singh

    2004-09-01

    correlação obtido pelo método de regressão linear foi de 0,9995 para o R-prop e de 0,9998 para o S-prop. A precisão intra-dia, expressa como desvio padrão relativo, foi menor do que 2%. A exatidão, determinada pela recuperação média de R- e S-prop nas amostras foi de 97,3% e 100,1% para a amostra comercial e de 99,5% e 100,4% para a amostra simulada, respectivamente. Excelentes níveis para os limites de detecção (1,34 ng e de quantificação (4,47 ng, além do tempo rápido de eluição dos enantiômeros do propranolol, confirmam a aplicabilidade do método no controle de qualidade de preparações farmacêuticas contendo este fármaco.

  14. Comportamento de variáveis fisiológicas em atletas de nado sincronizado durante uma sessão de treinamento na fase de preparação para as Olimpíadas de Atenas 2004 Comportamiento de variábles fisiológicas en atletas de nado sincronizado durante una sesion de entrenamiento en la fase de preparación para las Olimpíadas de Atenas 2004 Behavior of physiological variables in synchronized swimming athletes during a training session preparing for the Athens 2004 Olympic Games

    OpenAIRE

    Marina Guimarães Antunes Pazikas; Andréa Curi; Marcelo Saldanha Aoki

    2005-01-01

    O objetivo do presente estudo foi avaliar o comportamento de variáveis fisiológicas durante uma sessão de treino de nado sincronizado realizada na fase de preparação para as Olimpíadas de Atenas 2004. MATERIAIS E MÉTODOS: A amostra utilizada foi constituída pelo dueto (24 ± 0 anos) representante do Brasil em Atenas 2004. A coleta de dados foi realizada durante uma sessão de treinamento com duração de 198 minutos. O treino se iniciou com a parte física, seguida da parte técnica. Para a determi...

  15. 土家族初中生性知识、性态度问卷的编制及特点研究%The Development of the Questionnaire and Characteristic of Tu Jia Nationality Juniors' Knowledge of Sex and Attitude of Sex

    Institute of Scientific and Technical Information of China (English)

    葛缨; 赵耀

    2012-01-01

    Objective Based on interview, searching of references, counseling of experts, the Questionnaire of Tu Jia Nationality Juniors' Knowledge of Sex and Attitude of Sex was constructed. Methods Exploratory Factor Analysis and Confirmatory Factor Analysis of data from 790 Tu Jia nationality juniors showed that the Questionnaire of Tu Jia nationality juniors' Knowledge of Sex had 21 items, including four dimensions : physiological development, reproductive mystery, health care, venereal disease prevention; and the Questionnaire of Tu Jia nationality juniors' Attitude of Sex had 30 items, consisting of six dimensions: sex education attitude , sexual nature attitude, sexual morality attitude, sex description attitude , love attitude, unmarried sex attitude. Results The overall condition of knowledge of sex in all kinds of Tu Jia nationality juniors is good There are significant differences in sexual attitude among Tu Jia nationality juniors with various grades, residences, parents' educations. Conclusion The reliability and validity of the questionnaire are statistically reasonable.%目的 通过访谈、文献查阅、专家咨询等方式,编制了土家族初中生性知识、性态度问卷.方法 经过790名土家族初中生被试评定,对数据进行探索性因素分析和验证性因素分析显示,土家族初中生性知识问卷由21个项目组成,包括:生理发育、生殖奥秘、卫生保健、性病防治等4个因素;性态度问卷由30个项目组成,包括:性教育态度、性本质态度、性道德态度、性描写态度、谈恋爱态度、未婚性行为态度等6个因素.结果 土家族初中生各类群体对性知识了解总体情况较好,不同年级、居住地和父母文化程度的土家族初中生的性态度情况存在差异.结论问卷的信度与效度达到统计标准水平.

  16. ¿Somos los diseñadores sostenibles en nuestro trabajo? La mochila que cargan los objetos

    OpenAIRE

    Serrano Bulnes, Antonio

    2013-01-01

    Para que un objeto cualquiera sea sostenible debería tener en cuenta los tres aspectos que comporta la sostenibilidad: el impacto sobre la naturaleza, la pérdida de biodiversidad y la vulnerabilidad de los sistemas naturales. Según la definición que recoge el Informe Brundtland (1987) para la Comisión Mundial de Medio Ambiente y Desarrollo de las Naciones Unidas, "sostenibilidad es la característica según la cual pueden satisfacerse las necesidades de la población actual y local sin compromet...

  17. Uso do processo sol-gel na obtenção de materiais híbridos organo-inorgânicos: preparação, caracterização e aplicação em eletrólitos de estado sólido Utilization of the sol-gel process to obtain organic-inorganic hybrid materials: preparation, characterization and application as solid state electrolytes

    Directory of Open Access Journals (Sweden)

    Rita A Zoppi

    1997-12-01

    Full Text Available Neste trabalho é descrita a preparação de materiais híbridos constituídos de um copolímero orgânico contendo segmentos óxido de etileno e de sílica. A rede inorgânica foi formada na solução polimérica a partir da hidrólise e condensação do tetraetoxisilano, TEOS. Como copolímero foi utilizado o poli(óxido de etileno-b-amida-6, PEBAX. Foram preparados também híbridos contendo perclorato de lítio, denominados aqui híbridos eletrólitos. Os materiais foram caracterizados por microscopia eletrônica de transmissão, ensaios de tensão-deformação, análise dinâmico-mecânica e difratometria de raios-X. Os sistemas PEBAX/TEOS/LiClO foram também caracterizados por espectroscopia de impedância eletroquímica e voltametria cíclica para avaliação de suas propriedades como eletrólito de estado sólido.In this work it was described the preparation of hybrid materials constituted by a organic copolymer, which contains ethylene oxide blocks, and silica. The inorganic network was formed by the hydrolysis and condensation of tetraethoxysilane, TEOS, into the polymeric solution. As a copolymer, it was used poly(ethylene oxide-b-amide-6, PEBAX. Hybrids containing lithium perchlorate, known as hybrid electrolytes, were also prepared. The materials were characterized by transmission electron microscopy, stress-strain tests, dynamic-mechanical analysis and X-ray diffractometry. The PEBAX/TEOS/LiClO4 systems were also characterized by electrochemical impedance spectroscopy and cyclic voltammetry to evaluate their properties as a solid state electrolyte.

  18. Utilização de uma resina preparada pelo método da solução líquida de citratos como ligante durante a preparação de filtros cerâmicos à base de alumina Utilization of a resin, prepared by the citrate liquid solution method, as binder during the manufacturing of alumina-based ceramic filters

    Directory of Open Access Journals (Sweden)

    D. Gouvêa

    1999-05-01

    Full Text Available Este trabalho propõe a preparação de filtros cerâmicos macroporosos pela técnica da impregnação de espumas de poliuretano e utilização de uma resina preparada pelo método Pechini contendo íons alumínio e manganês como ligante. Nas temperaturas inferiores à de combustão da resina a parte orgânica age com ligante. Após a decomposição térmica, o produto da combustão, ou seja, alumina contendo íons Mn, passa a ser um ligante inorgânico mas apresentando composição química semelhante à do filtro. Os filtros assim preparados apresentaram três famílias de distribuições de tamanho de poros (50,2 e 0,2 mm e a densidade da estrutura do material, considerando-se apenas a porosidade referente as duas últimas distribuições, é de 87% da densidade teórica.This work proposes the utilization of a resin prepared via the Pechini method, bearing aluminum and manganese cations as binders. The filters prepared in this way present three families of pore size distributions (50, 2 and 0.2 mm and the resulting density of the material, considering only the apparent porosity of the last two distributions is 87% of the theoretical density.

  19. Utilização de argilas esmectíticas do nordeste do Brasil para preparação de um adsorvente organofílico Utilization of smectit clays from the northeast of Brazil for preparing an organophilic adsorber

    Directory of Open Access Journals (Sweden)

    J. V. F. L Cavalcanti

    2010-06-01

    Full Text Available O principal objetivo foi a preparação e a utilização de uma argila organofílica com capacidade de promover a separação do composto fenol, encontrado em larga escala nos efluentes aquosos de indústrias petroquímicas. O trabalho consistiu no preparo de um material adsorvente, partindo de cinco tipos diferentes argilas retiradas de bacias localizadas na região nordeste do Brasil: Verde Lodo, Bofe e Chocolate, retiradas do município de Campina Grande, Boa Vista, PB; e Lagoa de Dentro e S. Jorge, do Pólo Gesseiro do Araripe, PE. As argilas in natura foram tratadas com uma solução de carbonato de sódio visando a troca dos poli-cátions existentes em suas regiões intercamadas pelo cátion sódio, em um reator de bancada, com diferentes concentrações, tempo de contato e temperatura, de acordo com um planejamento experimental 2³. Como resposta ao tratamento com carbonato de sódio, fez-se a leitura das viscosidades plásticas (cP ou mPa.s das suspensões aquosas desse material com 4,86% em massa. Os resultados mostraram que a argila Chocolate, na melhor condição do planejamento experimental, atingiu uma viscosidade plástica de 4 mPa.s, sendo, portanto, escolhida como material adsorvente. Em seguida, este material foi submetido a um tratamento com sal quaternário de amônio em duas concentrações diferentes visando a troca catiônica de sódio por cátions amônio quaternário. Após o preparo do material adsorvente, foram realizados ensaios adsortivos de equilíbrio, sendo verificado o efeito da concentração do sal quaternário de amônio no processo adsortivo de remoção do fenol. A eficiência adsortiva, para uma relação de massa 1 g de argila organofílica e volume de solução fenólica igual a 100 mL, com concentrações iniciais entre 20 e 110 mg.L-1, foi de aproximadamente 70%.The goal of this research was the preparation and use of organophilic clay capable of promoting the separation of phenol, found in large scale

  20. Group Character Traits of Tu Jia Ethnic Minority under the Theory of“the Grand Five Character Traits”:From the Perspective of Folk Song of Tujia Nationality in Chongqing%“大五人格”理论下的土家族群体人格特征--重庆土家族民歌视角

    Institute of Scientific and Technical Information of China (English)

    彭福荣; 刘李慧子; 刘萍

    2014-01-01

    Special humane environment, ethnic minority group history and cultural tradition have cultivated the unique character traits of Tu Jia Ethnic Minority Group. Folk songs, featuring productive activities, male-female interactions, marital and funeral traditions and cultural communications all reflect those character traits. A case study of Tu Jia Ethnic Minority Group in Chongqing under the Theory of“the Grand Five Character Traits”reveals that the character traits of Tu Jia Ethnic Minority Group are exocentric, people-friendly, responsible, open-minded and squeamish.%特定人文环境、民族历史和文化传统形成了土家族独特的群体人格特征,重庆土家族在生产劳动、男女交往、婚丧习俗和文化交流等方面的民歌都透射出独特的群体人格。根据重庆土家族民歌的个案,运用人格心理学的“大五人格”理论,土家族群体人格特征具体表现为较高的外倾性、宜人性、尽责性、开放性及神经质等。

  1. Využití singulárních funkcí k výpočtu průhybu nosníku.

    OpenAIRE

    Fiala, Petr

    2008-01-01

    Účelem práce je porovnání dvou metod výpočtu ohybové čáry nosníku, řešení diferenciální rovnice ohybové čáry a metody založené na singulárních funkcích. Nosník je zatěžovám v pružné oblasti různými typy zatížení. Singulární funkce umožní stanovit jednotný postup řešení, nezávislý na kombinaci aplikovaného zatížení a je pro velmi výhodný pro další počítačové zpracování. The aim of this work is to compare two methods of calculation deflection curve of the beam, by means of the solution of di...

  2. Biscuits with No Added Sugar Containing Stevia, Coffee Fibre and Fructooligosaccharides Modifies α-Glucosidase Activity and the Release of GLP-1 from HuTu-80 Cells and Serotonin from Caco-2 Cells after In Vitro Digestion

    Science.gov (United States)

    Martinez-Saez, Nuria; Hochkogler, Christina Maria; Somoza, Veronika; del Castillo, Maria Dolores

    2017-01-01

    This study assessed the in vitro effects of the bioaccessible food components released during the simulated human digestion of a coffee fibre-containing biscuit (CFB) on α-glucosidase activity, antioxidant capacity and satiety hormones. Digest of CFB presented a significantly (p < 0.05) lower amount of sugar (68.6 mg/g) and a higher antioxidant capacity (15.1 mg chlorogenic acid eq./g) than that of a sucrose-containing biscuit (SCB). The CFB significantly reduced (p < 0.05) α-glucosidase activity (IC50 = 3.3 mg/mL) compared to the SCB (IC50 = 6.2 mg/mL). Serotonin and glucagon-like peptide-1 (GLP-1) release by differentiated Caco-2 and HuTu-80 cells, respectively, was stimulated by the CFB (355% at a concentration of 0.5 mg/mL and 278% at a concentration of 0.05 mg/mL) to the same order of magnitude as those of the SCB. To summarize, the CFB was demonstrated to reduce monosaccharide bioaccessibility, to inhibit a diabetes-related digestive enzyme, and to improve the release of satiety hormones. PMID:28677657

  3. Man of Good Metaphor is Good at Tao:The Art of Impartment of Master Tu Youguang's Academic Ideas%善喻者善道--涂又光先生学术思想的传达艺术

    Institute of Scientific and Technical Information of China (English)

    陈运超

    2015-01-01

    The art of imparting the academic ideas is necessary for a scholar qualified.Every master from the academia must have his extraordinary art of impartment.Tu Youguang is such a master unique for his metaphors.He is good at giving explicit instruction of his educational ideas through metaphors of prickles,rumination,grafting,magneto electricity,fellow students and fellow countrymen,mansion, train of pearls,dinner,nuclear reactor,cloth and clothe,and car,etc.,which are vivid,humorous and easy to accept.%学术传达素养是学者必备的基本素质。但凡学术大师必有极具个性的表达艺术,而比喻以生动、易记而成为最常用、也是最生动的表达方式之一。为学必为道,且善喻者善道。涂又光先生即是如此。他曾使用过泡菜喻、反刍喻、嫁接喻、磁电喻、同学与同乡喻、楼喻、珍珠链喻、餐喻、核反应堆喻、布料与成衣喻、车喻等,来进行自己的学术传达。

  4. Influência da nifedipina no bloqueio neuromuscular produzido pelo atracúrio e pelo cisatracúrio: estudo em preparações nervo frênico-diafragma de rato Influencia de la nifedipina en el bloqueo neuromuscular producido por atracurio y cisatracurio: estudio en preparación nervio frénico diafragma de ratón Influence of nifedipine on the neuromuscular block produced by atracurium and cistracurium: study in rat phrenic-diaphragmatic nerve preparation

    Directory of Open Access Journals (Sweden)

    Silmara Rodrigues de Sousa

    2006-04-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: Os bloqueadores de canais de cálcio podem interagir com bloqueadores neuromusculares potencializando seus efeitos. Os estudos sobre essa interação mostram resultados controversos. Em alguns estudos essas drogas produziram bloqueio neuromuscular, ou contratura, ou nenhum efeito sobre as respostas musculares esqueléticas foi evidenciado. O estudo avaliou, em diafragma de rato, os efeitos da nifedipina sobre a resposta muscular e sua possível interação com os bloqueadores neuromusculares. MÉTODO: Foram utilizados 25 ratos, com peso entre 250 e 300 g sacrificados sob anestesia com pentobarbital (40 mg.kg -1 por via intraperitonial. A preparação foi montada de acordo com a técnica descrita por Bulbring. O diafragma foi mantido sob tensão, ligado a um transdutor isométrico e submetido à estimulação indireta de 0,1 Hz de freqüência. As contrações do diafragma foram registradas em fisiógrafo. Para avaliação dos efeitos das drogas na transmissão neuromuscular, estas foram adicionadas isoladamente ou associadas à preparação, nas seguintes concentrações: nifedipina (4 µg.mL-1; atracúrio (20 µg.mL-1; cisatracúrio (3 µg.mL-1. Nas preparações nervo frênico-diafragma avaliaram-se: 1 a amplitude das respostas do músculo diafragma à estimulação indireta, antes e 45 minutos após a adição da nifedipina e dos bloqueadores neuromusculares isoladamente e após a associação das drogas; 2 os efeitos da nifedipina nos potenciais de membrana (PM e potenciais de placa terminal em miniatura (PPTM. RESULTADOS: A nifedipina empregada isoladamente não alterou a amplitude das respostas musculares, mas aumentou significativamente a atividade bloqueadora neuromuscular do atracúrio e do cisatracúrio. Não alterou o potencial de membrana e ocasionou aumento inicial na freqüência dos PPTM, seguido de bloqueio. CONCLUSÕES: A nifedipina na concentração empregada potencializou o bloqueio neuromuscular

  5. Los narûs (kudurrus babilónicos del Bronce Final y el Hierro = The Babylonian narû (kudurrētu in Late Bronze and Iron Age

    Directory of Open Access Journals (Sweden)

    Sara Arroyo Cuadra

    2016-11-01

    Full Text Available Los narû babilónicos, conocidos tradicionalmente con el nombre de kudurrētu (en singular kudurru, fueron unos monumentos de piedra que incluían representaciones divinas encarnadas generalmente por sus emblemas y escenas con figuras antropomorfas, junto a un texto administrativo sobre transacciones  de tierras. Estos narû vieron la luz con la Dinastía Casita de Babilonia y dejaron de utilizarse en el período Neo-babilónico (ca. 1400-650 a.C.. La gran mayoría de los ejemplares se ha descubierto en Susa en contextos arqueológicos descontextualizados, pero los ejemplares hallados in situ nos hablan de que su dispersión geográfica original hay que situarla en el territorio de la Baja Mesopotamia. Hemos emprendido una investigación orientada a estudiar el corpus de estos monumentos integrado hasta el momento por 178 ejemplares, adoptando una postura interdisciplinar que incluye el análisis de la información que transmiten sus textos, iconografía, materialidad y contexto arqueológico. Por ello, a lo largo del presente artículo se intentará definir el criterio de adscripción de los ejemplares conocidos a dicho corpus según sus propias inscripciones y los estudios previos de  otros autores. A continuación, se hablará de las circunstancias que pudieron motivar su aparición y de los eventos histórico-políticos que pudieron influir en su evolución, así como del ámbito geográfico en que estuvieron vigentes. Después, se analizará el contenido de sus inscripciones y la simbología de su iconografía para, aportar, finalmente, un estudio comparativo entre textos y símbolos, quedando todo ello plasmado en un apartado final de conclusiones.Babylonian narû, traditionally named kudurrētu, were stone monuments with divine representations (generally through its divine emblems and figurative scenes with anthropomorphic figures, together with an administrative text about a land grant. These narû originated in Kassite Babylon and

  6. La dissimilació del jo vs tu en la poesia de Maria-Mercè Marçal. Anàlisi comparativa entre les sextines de Terra de Mai i Sang presa

    Directory of Open Access Journals (Sweden)

    Marta Font Espriu

    2010-01-01

    Full Text Available La construcció d’un espai propi de la dona dins de la poesia, dita, amorosa, no només implica una ordenació nova del jo i del tu sinó que implica, de forma inexorable, la recreació del llenguatge poètic. Maria-Mercè Marçal usa el símbol del mirall per expressar l‘equivalència física dels cossos amants/amats i per extremar la fusió tant física com amorosa que es convertirà en l’acció transformadora i afirmativa de la identitat. La reciprocitat entre el jo poètic i la seva altra es va teixint amb l’ús d’imatges com l’aigua, la sang, la suor, etc., les quals han rebut, tradicionalment, un caràcter abjecte, i Marçal les situa en un primer pla, mostrant la seva expressivitat germinal i pregona. En un segon moment, la separació i la nostàlgia bolquen el poema cap a un jo poètic mutilat, que cerca un cos absent i l’espai de plenitud d’un jo que es troba irreparablement ferit. Hi ha tota una destil·lació d’un món poètic nou, que culmina l’assoliment d’un espai literari femení i lèsbic, creador de nous àmbits per a l’expressió del desig, definitivament conquerits per Marçal i lliurats a la poesia catalana.

  7. Chair in open-cast and international mining at the Institute for Mining at Clausthal Technological University; Lehrstuhl fuer Tagebau und Internationaler Bergbau am Institut fuer Bergbau der TU Clausthal

    Energy Technology Data Exchange (ETDEWEB)

    Tudeshki, H.; Hardebusch, T. [Lehrstuhl fuer Tagebau und Internationaler Bergbau am Institut fuer Bergbau, TU Clausthal-Zellerfeld (Germany)

    2002-12-05

    The range of activities of the Chair in Open-cast and International Mining at the Institute for Mining at Clausthal Technological University currently comprises the field of teaching and further scientific training, research and development as well as transfer of knowledge and technology. In addition to these legally sanctioned activities PR work concerning the necessity for the mining of mineral raw materials and the associated training of mining engineers plays a central role. In accordance with the fundamental idea that a prerequisite for good training in the field of engineering science is a close link between university and industry the contents of the courses are always adapted to the state of the art in the mining industry and the main areas of research based on practical requirements. In accordance with this process current aspects of the safeguarding of raw materials, sustenance and management of knowledge in the mining industry are integrated in these courses. The current research work is interdisciplinary and deals with quality management, deposit protection, recultivation and rehabilitation as well as reduction of emissions. (orig.) [German] Das Aufgabenspektrum des Lehrstuhls fuer Tagebau und Internationalen Bergbau am Institut fuer Bergbau der TU Clausthal umfasst die Gebiete Lehre und wissenschaftliche Weiterbildung, Forschung und Entwicklung sowie Wissens- und Technologietransfer. Ueber diese gesetzlich verankerten Aufgaben hinaus nimmt die PR-Arbeit ueber die Notwendigkeit bergmaennischer Gewinnung mineralischer Rohstoffe und der damit einhergehenden Ausbildung von Bergbauingenieuren eine zentrale Stellung ein. Dem Leitgedanken folgend, dass eine gute Ausbildung im ingenieurwissenschaftlichen Bereich eine enge Verbindung zwischen Hochschule und Industrie voraussetzt, werden die Lehrinhalte stets dem Stand der Technik im Bergbau angepasst und die Forschungsschwerpunkte praxisbezogen abgeleitet. Diesem Prozess folgend wurden aktuell Aspekte der

  8. Tu Salud, ¡Si Cuenta!: Exposure to a community-wide campaign and its associations with physical activity and fruit and vegetable consumption among individuals of Mexican descent.

    Science.gov (United States)

    Reininger, Belinda M; Mitchell-Bennett, Lisa; Lee, MinJae; Gowen, Rose Z; Barroso, Cristina S; Gay, Jennifer L; Saldana, Mayra Vanessa

    2015-10-01

    Mexican Americans along the US-Mexico border have been found to be disproportionately affected by chronic diseases particularly related to lack of physical activity and healthful food choices. A community-wide campaign (CWC) is an evidence-based strategy to address these behaviors but with few examples of implementation in Mexican descent populations facing profound health disparities. We examined exposure to a CWC, titled Tu Salud ¡Sí Cuenta!, and its association with meeting the recommended minutes of moderate and vigorous physical activity weekly and consuming more portions of fruits and vegetables daily. A cross-sectional sample of 1438 Mexican descent participants was drawn from a city-wide, randomly-selected cohort interviewed between the years 2008 and 2012. Multivariable comparisons of participants exposed and not exposed to the CWC and meeting physical activity guidelines or their fruit and vegetable consumptions using mixed effects models were conducted. The community-wide campaign components included different forms of mass media and individually-focused components such as community health worker (CHW) home visits. After adjusting for gender, age, marital status, educational attainment, language preference, health insurance, and diabetes diagnosis, the strongest association was found between meeting physical activity guidelines and exposure to both CHW discussions and radio messages (adjusted OR = 3.83; 95% CI = [1.28, 6.21]; p = 0.0099). Participants who reported exposure to both radio and TV messages consumed more portions of fruits and vegetables than those who reported no exposure (adjusted RR = 1.30; 95% CI = [1.02, 1.66]; p = 0.0338). This study provides insights into the implementation and behavioral outcomes associated with exposure to a community-wide campaign, a potential model for addressing lifestyle modifications in populations affected by health disparities. Copyright © 2015 Elsevier Ltd. All rights reserved.

  9. Influence of the preparation method on the low efficacy of multi-herb commercial products: the example of João da Costa e Associações Influência do método de preparação na baixa eficácia de produtos fitoterápicos: o exemplo do João da Costa e Associações

    Directory of Open Access Journals (Sweden)

    Maria G. L. Brandão

    2010-05-01

    antinociceptiva. Já a composição química foi determinada por analises em HPLC/ DAD. Uma fraca inibição da segunda fase da nocicepção foi observada no teste da formalina, indicando uma ação semelhante aos antiinflamatórios esteroidais e não esteroidais. Apesar de ser preparado pela decocção de cinco plantas, a análise no HPLC apresentou somente dois picos, e nenhum deles correspondeu aos componentes observados nos extratos etanólicos preparados com as mesmas plantas. Os resultados sugerem que o método de preparação de JCA promove a perda dos componentes químicos das plantas e interfere consideravelmente na eficácia do produto.

  10. 西藏西部东嘎1号窟法界语自在曼荼罗图像与文本%On the Iconographies of the Dharma-dhātu-vāgi vara-ma ala from Dung-dkar Cave 1 in Western Tibet and the Corresponding Texts

    Institute of Scientific and Technical Information of China (English)

    王瑞雷

    2013-01-01

    以东嘎1号窟法界语自在曼荼罗壁画为对象,梳理与该曼荼罗相关的藏文文献,并结合该窟法界语自在曼荼罗图像作对比研究,理清11世纪初至12世纪中晚期在西藏西部流行的以四面八臂白色文殊菩萨为主尊的法界语自在曼荼罗图像与所据文本之间的关系。%Focusing on the wall paintings of the Dharma-dhātu-vāgi varama ala in Dung-dkar Cave 1, this study sorts out the Tibetan documents about this kind of mandala and presents a comparison of both the texts and the iconographies, so as to clarify the relationship between the iconographies of Dharma-dhātu-vāgi varama ala dominated by a four-headed eight-armed white Manjusri image, which was popular in western Tibet from the early 11th century to the middle of the 12th century, and the texts they were based on.

  11. Pourquoi viens-tu si Tarde ?

    OpenAIRE

    Latour, Bruno

    2013-01-01

    J’ai toujours cru, je l’avoue, que le réel était rationnel et que les bons auteurs, en fin de compte, finissaient toujours par triompher. La publication récente des œuvres de Gabriel Tarde, sociologue et métaphysicien, suffit à prouver le contraire. Voilà une tradition scientifique en sociologie disparue aussi sûrement que Jean-Baptiste Lully. Priver les sociologues d’une pareille philosophie, n’est-ce pas aussi grave que d’avoir privé les amateurs d’art de musique baroque ? Qui sait ce que l...

  12. Dengue — Quo tu et quo vadis?

    Directory of Open Access Journals (Sweden)

    Nikos Vasilakis

    2011-09-01

    Full Text Available Dengue viruses (DENV are by far the most important arboviral pathogens in the tropics around the world, putting at risk of infection nearly a third of the global human population. DENV are members of the genus Flavivirus in the Family Flaviviridae and comprise four antigenically distinct serotypes (DENV-1-4. Although they share almost identical epidemiological features, they are genetically distinct. Phylogenetic analyses have revealed valuable insights into the origins, epidemiology and the forces that shape DENV evolution in nature. In this review, we examine the current status of DENV evolution, including but not limited to rates of evolution, selection pressures, population sizes and evolutionary constraints, and we discuss how these factors influence transmission, pathogenesis and emergence.

  13. TuLiP : reshaping trust management

    NARCIS (Netherlands)

    Czenko, M.R.

    2009-01-01

    In today's highly distributed and heterogeneous world of the Internet, sharing resources has become an everyday activity of every Internet user. We buy and sell goods over the Internet, share our holiday pictures using facebook (TM), "tube" our home videos on You Tube (TM), and exchange our

  14. TuLiP : reshaping trust management

    OpenAIRE

    Czenko, M.R.

    2009-01-01

    In today's highly distributed and heterogeneous world of the Internet, sharing resources has become an everyday activity of every Internet user. We buy and sell goods over the Internet, share our holiday pictures using facebook (TM), "tube" our home videos on You Tube (TM), and exchange our interests and thoughts on blogs. We podcast, we are Linkedin (TM) to extend our professional network, we share files over P2P networks, and we seek advice on numerous on-line discussion groups. Although in...

  15. TU-AB-BRD-01: Process Mapping

    Energy Technology Data Exchange (ETDEWEB)

    Palta, J. [Virginia Commonwealth University (United States)

    2015-06-15

    Current quality assurance and quality management guidelines provided by various professional organizations are prescriptive in nature, focusing principally on performance characteristics of planning and delivery devices. However, published analyses of events in radiation therapy show that most events are often caused by flaws in clinical processes rather than by device failures. This suggests the need for the development of a quality management program that is based on integrated approaches to process and equipment quality assurance. Industrial engineers have developed various risk assessment tools that are used to identify and eliminate potential failures from a system or a process before a failure impacts a customer. These tools include, but are not limited to, process mapping, failure modes and effects analysis, fault tree analysis. Task Group 100 of the American Association of Physicists in Medicine has developed these tools and used them to formulate an example risk-based quality management program for intensity-modulated radiotherapy. This is a prospective risk assessment approach that analyzes potential error pathways inherent in a clinical process and then ranks them according to relative risk, typically before implementation, followed by the design of a new process or modification of the existing process. Appropriate controls are then put in place to ensure that failures are less likely to occur and, if they do, they will more likely be detected before they propagate through the process, compromising treatment outcome and causing harm to the patient. Such a prospective approach forms the basis of the work of Task Group 100 that has recently been approved by the AAPM. This session will be devoted to a discussion of these tools and practical examples of how these tools can be used in a given radiotherapy clinic to develop a risk based quality management program. Learning Objectives: Learn how to design a process map for a radiotherapy process Learn how to perform failure modes and effects analysis analysis for a given process Learn what fault trees are all about Learn how to design a quality management program based upon the information obtained from process mapping, failure modes and effects analysis and fault tree analysis. Dunscombe: Director, TreatSafely, LLC and Center for the Assessment of Radiological Sciences; Consultant to IAEA and Varian Thomadsen: President, Center for the Assessment of Radiological Sciences Palta: Vice President of the Center for the Assessment of Radiological Sciences.

  16. TU students sweep UAV design competition

    NARCIS (Netherlands)

    Klein, R.; Brust, S.; Brown, M.; Vos, R.

    2013-01-01

    To design a high-altitude long-endurance UAV to fly a twenty-hour reconnaissance mission at an altitude of 80,000ft, was the assignment for this year’s undergraduate design competition. The competition was organized by the American Institute of Aeronautics and Astronautics (AIAA). Three students fro

  17. TuLip : reshaping trust management

    NARCIS (Netherlands)

    Czenko, Marcin Ryszard

    2009-01-01

    In today’s highly distributed and heterogeneous world of the Internet, sharing resources has become an everyday activity of every Internet user. We buy and sell goods over the Internet, share our holiday pictures using facebook™, “tube” our home videos on You Tube™, and exchange our interests and th

  18. Tu-144LL Rollout Ceremony Audience

    Science.gov (United States)

    1995-01-01

    A modified Russian Supersonic Passenger Jet is rolled out of its hangar at the Zhukousky Airfield March 17, 1996 to symbolize the start of a joint six-month research program between NASA and U.S. Industry Team and the Russian Aeronautics Establishment

  19. La ayahuasca te da tu arte

    Directory of Open Access Journals (Sweden)

    Paolo del Aguila

    2014-09-01

    Full Text Available Para Paolo del Águila, descendiente del pueblo asháninka, la creatividad es un don de las plantas. Durante su primera experiencia visionaria, la ayahuasca personificada en el cuerpo de anciana le entregó unos pinceles y un estilo propio, liberándolo de las disputas y los convencionalismos que había encontrado en la escuela de arte en Pucallpa. Desde entonces, pintura y vida son indisociables y su objetivo como artista es comunicar al público las experiencias espirituales que le acontecieron en un ámbito normalmente invisible que él se esfuerza por visibilizar.

  20. La grammaire, tu piges ? niveau 2

    CERN Document Server

    Granger, Colin

    1997-01-01

    This French resource is one of a series of stand-alone photocopiable materials designed to supplement any modern-language course. The activities are suitable for individuals, pairs, groups and whole-class language practice.

  1. La grammaire, tu piges ? niveau 1

    CERN Document Server

    Granger, Colin

    1997-01-01

    This French resource is one of a series of stand-alone photocopiable materials designed to supplement any modern-language course. The activities are suitable for individuals, pairs, groups and whole-class language practice.

  2. Catalytic pyrolysis of Huang Tu Miao coal: TG-FTIR study%应用TG-FTIR技术研究黄土庙煤催化热解特性

    Institute of Scientific and Technical Information of China (English)

    李爽; 陈静升; 冯秀燕; 杨斌; 马晓迅

    2013-01-01

    用浸渍法制备过渡金属氧化物担载型催化剂MOx/USY(M=Co、Mo、Co-Mo),用热重红外联用技术考察了MOx/USY催化剂对黄土庙(HTM)煤热解失重特性和热解产物生成规律的影响.热重实验结果表明,MOx/USY催化剂可使HTM煤热解的二次脱气条件更为温和,热解峰温分别提前14、23和9℃.动力学分析结果表明,MOx/USY催化剂可降低HTM煤样热解的活化能.FT-IR研究表明,MOx/USY催化剂可有效改善HTM煤热解产物的组成和分布,CoOx/USY催化剂能显著提高HTM煤热解产物中高热值气体(CO、CH4)和轻质芳烃以及脂肪烃类化合物的含量;MoOx/USY催化剂没有明显改善HTM煤热解产物组成和分布;MoOx-CoOx/USY催化剂可促进CO、CH4、轻质芳烃和脂肪烃类化合物的生成,却使热解产物的生成向高温区移动,说明USY负载的不同过渡金属氧化物对煤样热解行为和热解产物有较大影响.%MOx/USY catalysts ( M = Co, Mo, Co-Mo) were prepared by incipient wetness impregnation method. Catalytic pyrolysis of Huang Tu Miao (HTM) coal was investigated and the pyrolysis products were examined by TG-FTIR technique. TG results indicate that MOx/USY catalysts are effective in lowering degasifiction temperature (14, 23 and 9℃ respectively) in HTM coal pyrolysis. Kinetic calculations show that MOx/USY catalysts are effective in lowing activation energy of pyrolysis. FT-IR analyses show that the MOX/ USY catalysts are effective in manipulating coal pyrolysis products. CoOx/USY catalyst promote the generation of CH4, CO and aromatic hydrocarbons and aliphatic hydrocarbons in HTM coal pyrolysis. MoOx/USY catalyst show little effect in manipulating pyrolysis products. CoOx-MoOx/USY catalyst is a promising catalyst in giving high yield of volatile products, however the pyrolysis temperature moved to high temperature region. These results indicate that different metal oxides incorporated zeolite USY have different impact on manipulating

  3. Influência da lidocaína no bloqueio neuromuscular produzido pelo rocurônio: estudo em preparação nervo frênico-diafragma de rato Influencia de la lidocaína en el bloqueo neuromuscular producido por el rocuronio: estudio en preparación nervio frénico-diafragma de ratón Influence of lidocaine on the neuromuscular block produced by rocuronium: study in rat phrenic-diaphragmatic nerve preparation

    Directory of Open Access Journals (Sweden)

    Yolanda Christina S. Loyola

    2006-04-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O mecanismo de ação dos anestésicos locais (AL na junção neuromuscular motivou a realização de vários estudos. Em baixas doses eles não interferem na transmissão neuromuscular, mas em altas doses podem comprometer a transmissão neuromuscular e potencializar os efeitos de bloqueadores neuromusculares. O objetivo do estudo foi avaliar, em diafragma de rato, a interação da lidocaína com o rocurônio através da influência no grau de bloqueio neuromuscular. MÉTODO: Foram utilizados ratos, com peso entre 250 e 300 g. A preparação foi feita de acordo com a técnica descrita por Bulbring. Formaram-se grupos (n = 5 de acordo com a droga em estudo: lidocaína - 20 µg.mL-1 (Grupo I; rocurônio - 4 µg.mL-1 (Grupo II e rocurônio - 4 µg.mL-1 com lidocaína - 20 µg.mL-1 (Grupo III. Foram avaliadas: 1 a amplitude das respostas do músculo diafragma à estimulação indireta, antes e 60 minutos após a adição da lidocaína e do bloqueador neuromuscular; 2 os potenciais de membrana (PM e potenciais de placa terminal em miniatura (PPTM; 3 a eficácia da neostigmina e 4-aminopiridina na reversão do bloqueio neuromuscular. RESULTADOS: A lidocaína isoladamente não alterou a amplitude das respostas musculares. Com o uso prévio de lidocaína o bloqueio neuromuscular do rocurônio foi de 82,8% ± 1,91%, com diferença significativa (p = 0,0079 em relação ao grupo com rocurônio isolado (57,8% ± 1,9%. O bloqueio foi parcial e totalmente revertido pela neostigmina e 4-aminopiridina, respectivamente. A lidocaína não alterou o potencial de membrana e ocasionou aumento inicial na freqüência dos PPTM, seguido de bloqueio. CONCLUSÕES: A lidocaína potencializou o bloqueio neuromuscular produzido pelo rocurônio. As alterações do PPTM identificam ação pré-sináptica. O antagonismo completo da 4-aminopiridina sugere componente pré-sináptico, idéia que é suportada pelo antagonismo parcial pela neostigmina

  4. Influência do lítio no bloqueio neuromuscular produzido pelo atracúrio e pelo cisatracúrio: estudo em preparações nervo frênico-diafragma de rato Influencia del litio en el bloqueo neuromuscular producido por el atracurio y por el cisatracurio: estudio en preparo nervio frénico-diafragma del ratón Influence of lithium on the neuromuscular blockade produced by atracurium and cisatracurium: study on rat phrenic nerve-diaphragm preparations

    Directory of Open Access Journals (Sweden)

    Samanta Cristina Antoniassi Fernandes

    2007-06-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: O lítio, fármaco amplamente utilizado nos distúrbios bipolares, pode interagir com os bloqueadores neuromusculares. Os mecanismos para explicar os seus efeitos na transmissão neuromuscular e a interação com bloqueadores neuromusculares são controversos. O objetivo deste trabalho foi avaliar, em diafragma de rato, os efeitos do lítio sobre a resposta muscular à estimulação indireta e a possível interação com os bloqueadores neuromusculares. MÉTODO: Utilizaram-se ratos com peso entre 250g e 300g, sacrificados sob anestesia com uretana. A preparação nervo frênico-diafragma foi montada de acordo com a técnica descrita por Bulbring. O diafragma foi mantido sob tensão, ligado a um transdutor isométrico e submetido à estimulação indireta de 0,1 Hz de freqüência. As contrações do diafragma foram registradas em fisiógrafo. Da análise da amplitude das respostas musculares avaliaram-se: os efeitos dos fármacos: lítio (1,5 mg.mL-1; atracúrio (20 µg.mL-1 e cisatracúrio (3 µg.mL-1 empregados isoladamente; da associação lítio-bloqueadores neuromusculares; e do lítio no bloqueio neuromuscular produzido pelo atracúrio (35 µg.mL-1 e cisatracúrio (5 µg.mL-1. Os efeitos foram avaliados antes e 45 minutos após a adição dos fármacos. Também foram estudados os efeitos do lítio nos potenciais de membrana (PM e potenciais de placa terminal em miniatura (PPTM. RESULTADOS: O lítio isoladamente não alterou a amplitude das respostas musculares, mas diminuiu significativamente o bloqueio neuromuscular produzido pelo atracúrio e cisatracúrio. Não alterou o PM e ocasionou aumento inicial da freqüência dos PPTM. CONCLUSÕES: O lítio empregado isoladamente não comprometeu a transmissão neuromuscular e aumentou a resistência ao efeito do atracúrio e cisatracúrio. Não mostrou ação sobre a fibra muscular, sendo que as alterações nos potenciais de placa terminal em miniatura evidenciaram a

  5. Determination, by using GPR, of the volumetric water content in structures, sub-structures, foundations and soil - ongoing activities in Working Project 2.5 of COST Action TU1208

    Science.gov (United States)

    Tosti, Fabio; Slob, Evert

    2015-04-01

    This work will endeavour to review the current status of research activities carried out in Working Project 2.5 'Determination, by using GPR, of the volumetric water content in structures, sub-structures, foundations and soil' within the framework of Working Group 2 'GPR surveying of pavements, bridges, tunnels and buildings; underground utility and void sensing' of the COST (European COoperation in Science and Technology) Action TU1208 'Civil Engineering Applications of Ground Penetrating Radar' (www.GPRadar.eu). Overall, the Project includes 55 Participants from over 21 countries representing 33 Institutions. By considering the type of Institution, a percentage of 64% (35 units) comes from the academic world, while Research Centres and Companies include, respectively, the 27% (15 units) and 9% (5 units) of Institutions. Geographically speaking, Europe is the continent most represented with 18 out of 21 countries, followed by Africa (2 countries) and Asia (1 country). In more details and according to the Europe sub-regions classification provided by the United Nations, Southern Europe includes 39% of countries, Western Europe 27%, while Northern and Eastern Europe are equally present with 17% of countries each. Relying on the main purpose of Working Project 2.5, namely, the ground-penetrating radar-based evaluation of volumetric water content in structures, substructures , foundations, and soils, four main issues have been overall addressed over the first two years of activities. The first one, has been related to provide a comprehensive state of the art on the topic, due to the wide-ranging applications covered in the main disciplines of civil engineering, differently demanding. In this regard, two main publications reviewing the state of the art have been produced [1,2]. Secondly, discussions among Working Group Chairs and other Working Project Leaders have been undertaken and encouraged to avoid the risk of overlapping amongst similar topics from other Working

  6. CADEIRA E MOCHILA ESCOLARES NO PROCESSO DE DESENVOLVIMENTO DA MÁ POSTURA E POSSÍVEIS DEFORMIDADES EM CRIANÇAS DE 8-11 ANOS

    Directory of Open Access Journals (Sweden)

    Vanessa Santhiago

    2009-06-01

    Full Text Available The population corporal posture concern is currently increasing. Students are maintained at classrooms in uncomfortable and inadequate positions for long time in during days, weeks, months and years. As a consequence, these students can develop bad postures. This study was accomplished during November, 2005 period, in 11 schools of 7 cities, with 330 students of both sexes with 8-11 years, which there were performed chairs utilization per students. It was verified that more studies are necessary about the chairs measure to verify comparative data for that age group, however it seems to be inadequate for the students. Regarding the backpacks, was found that the load transported by the students is extremely high, and in this sense, both can contribute to the bad posture and possible development of deformities postures.

  7. Las cosas que se llevan en la mochila: El poder en comunidades de okupación rural españolas

    Directory of Open Access Journals (Sweden)

    María Teresa Ramírez

    2013-07-01

    Full Text Available En este texto presento algunas reflexiones sobre los discursos que buscan generar una alternativa al modelohegemónico propuesto por el neoliberalismo. Para esto, me centro en el discurso sobre el poder de un grupo depersonas vinculadas al movimiento de okupación rural español. Comienzo desde las motivaciones que tienen laspersonas de este grupo para buscar una forma de vida cotidiana alternativa a la hegemónica, y las dificultades,centradas en la propia comunidad, con las que se han encontrado para lograrlo. Desde aquí, expongo las concepcionesque tienen sobre el poder, utilizando como marco interpretativo la teoría de Foucault. Luego me centroen la forma en que este grupo busca superar estas dificultades, principalmente vinculada al “reconocimientoindividual” de las emociones. Sobre este punto termino con algunas reflexiones críticas ligadas a conceptoscomo tecnologías del yo y subjetivación.

  8. Negligência da Omissão: de Onde Tu Vens, Pra Onde Tu Vais?

    Directory of Open Access Journals (Sweden)

    Rejane Alexandrina Domingues Pereira do Prado

    2015-06-01

    Full Text Available Este ensaio tem como objetivo discutir a produção científica sobre negligência da omissão de informações, visando apresentar uma agenda de pesquisa referente ao tema a partir das direções indicadas pelo estado da arte das pesquisas sobre o fenômeno. O ensaio objetiva, ainda, apresentar e caracterizar as perspectivas teóricas que prevalecem nessa produção. Por meio da revisão das produções acadêmicas nacional e internacional, publicadas entre 1988 e 2014, foram selecionados todos os artigos encontrados, sendo estes, no total, 19 trabalhos. Identificou-se, portanto, que os estudos sobre o fenômeno da negligência da omissão são ainda seminais e a literatura carece de pesquisas adicionais para melhor entendimento do fenômeno. Por meio do método da leitura científica, todos os 19 artigos encontrados foram analisados e, ao final, propõe-se uma agenda de pesquisa sobre o tema da negligência da omissão, composta a partir dos gaps identificados nos estudos que trataram dessa temática.

  9. Co mbi nation of renal artery cold perfusion with laparoscopic partial nephrecto my for co mplex renal tu mor:our i nitial experience%肾动脉低温灌注联合腹腔镜肾部分切除术处理复杂肾肿瘤的初步经验

    Institute of Scientific and Technical Information of China (English)

    马鑫; 刘新; 郑涛; 史涛坪; 瓦斯里江·瓦哈甫; 俞鸿凯; 宋鹏; 张旭

    2014-01-01

    目的:介绍肾动脉低温灌注联合腹腔肾部分切除术处理复杂肾肿瘤的初步经验.方法:2013年3月起,共收治复杂肾肿瘤患者14例,在肾动脉低温灌注下,7例采用腹腔镜经腹膜后入路行肾部分切术,7例采用 da Vi nci Si 机器人经腹腔入路实施手术.所有术后均由同一术者完成.结果:14例患者中12例成功实施了肾动脉低温灌下的肾部分切除术,术中4例出现了动脉阻断不全,2例术中改为肾根治性切除术,无一例改为开放手术.12例肾部分切除术的患者术后出现轻度肾功能下降.结论:对于复杂肾脏肿瘤,肾动脉低温灌注联合腹腔镜肾部分切除术具有可行性和安全性,可有效减少根治性肾切除的发生和热缺血对肾脏的损害.%To introduce our initial experience in using renal artery cold perfusi on in laparoscopic partial nephrecto my for complex renal tu mor .Methods :From March 201 3 ,1 4 patients with complex renal tu mor under went partial nephrecto my combined with renal artery col d perfusi on .The operati ons were carried out by the sa me surgeon using laparoscopic retroperitoneal approach (n =7 )and robotic transperitoneal approach (n =7 ).Results :Partial ne-phrecto my was successfull y perfor med on 1 2 patients .incomplete arterial bl ock occurred in 4 patients ,and 2 patients under went conversi on to radical nephrecto my .There was no conversi on to open surgery in all cases .Additi onall y ,a mil d renal dysfuncti on was i dentified in the 1 2 patients who under went partial nephrecto my .Conclusions :Co mbina-ti on of renal artery cold perfusi on with laparoscopic partial nephrecto my is feasi ble and safe for complex renal tu mor . It can reduce the inci dence of radical nephrecto my ,as well as the da mage of war mische mia to renal functi on .

  10. 广西土司制度的历史渊源与改土归流开始的时间问题辨析%On Historical Origination of Tusi System and Beginning - Time of Gai Tu Gui Liu in Guangxi

    Institute of Scientific and Technical Information of China (English)

    蓝武

    2012-01-01

    广西土司制度最终确立于元代。从历史发展的观点来看,广西土司制度产生的源头大体可以追溯到秦汉王朝的“羁縻之治”时代,唐宋羁縻州县制度是广西土司制度的前身,元明广西土司制度由唐宋羁縻州县制度发展与演变而来,两者之间是“源”与“流”的关系。从严格意义上说,广西改土归流开始的时间是在明朝统一广西之初,具体表现为明初在统一广西的过程中实施对钦州七峒长官司的改流,由此揭开了王朝中央在广西民族地区实施改土归流的序幕。历史渊源与改土归流开始的时间问题由此成为土司制度研究中的重要课题。%Tusi ( local tribe chief) system in Guangxi was formally established in Yuan dynasty. Viewed historically, The system can be originated from Ji Mi policy (a mollification policy for mi-norities or frontier regions in ancient China) in QinHan dynasty, while Ji Mi administration system ( a system similar to Jimi Policy) in Tang - Song dynasty is the predecessors of Tusi system inGuangxi, and Tusi system in Yuan- Ming dynasty is the development and evolution of Ji Mi policy in Tang - Song dynasty. Practice of Gai Tu Gui Liu ( Practice of changing local tribe administrationto flexible administration) in Qidong area, Qinzhou, happened during the unification of Guangxi in early Ming dynasty and started the whole reform practiced by central government in minority regionsin Guangxi. Therefore, the historical origination and the beginning - time of Gai Tu Gui Liu are import issues in the study of Tusi system.

  11. Misturas NR/SBR: modos de preparação e propriedades

    Directory of Open Access Journals (Sweden)

    Visconte Leila L. Y.

    2001-01-01

    Full Text Available Aplicações de artefatos de borracha muitas vezes requerem um conjunto de propriedades que não podem ser fornecidas por um único elastômero sendo, então necessário, que misturas de dois ou mais polímeros sejam empregadas. Nesses casos, os demais ingredientes que normalmente são adicionados, distribuem-se de modo diferente dependendo de sua afinidade com cada uma das borrachas. O grau de dispersão de cada um desses ingredientes em cada fase elastomérica irá influenciar a velocidade e o grau de vulcanização e, certamente, terá conseqüências sobre o desempenho do composto final. Neste trabalho, a borracha natural (NR foi misturada ao elastômero de butadieno-estireno (SBR na proporção de 1:1 em peso. As composições foram preparadas em misturador de cilindros, segundo a norma ASTM D 3182, empregando-se quatro maneiras distintas para a incorporação dos aditivos. Após a vulcanização foram estudadas propriedades mecânicas, como resistências à tração e ao rasgamento e dureza, propriedades termo-dinâmico-mecânicas e a morfologia de cada uma das composições. As propriedades mecânicas foram avaliadas de acordo com as normas ASTM específicas para cada ensaio. Os resultados mostram que, apesar de se usar a mesma formulação, o modo de preparo tem grande influência sobre as propriedades, o que é detectável pelas propriedades mecânicas e pela análise morfológica, e evidenciado através de testes sensíveis, como o dinâmico-mecânico. No caso da mistura NR/SBR as melhores propriedades são obtidas quando é favorecida a vulcanização da borracha que, isoladamente, apresenta propriedades inferiores.

  12. Condições de estabilidade para preparações magistrais contendo clorexidina

    Directory of Open Access Journals (Sweden)

    Paula Martinez Rodrigue

    2009-12-01

    Full Text Available The mouthwash containing chlorhexidine (CHX is a solution and aims to complement the oral hygiene. It is considered standard for judging of antimicrobial effectiveness compared to other chemical agents in dentistry. CHX used in low concentrations it has a bacteriostatic and bactericidal high. The mouthwash consisting of CHX has some side effects, including blackening of the teeth, tongue and resets, burning mouth and changes in taste and to reverse the process, no need of stopping treatment. There are several factors that may interfere with the stability of CHX, including pH, light, and presence of substances with anionic character in the composition and packing in containers inadequate. His prolonged substantivity accounts for their effectiveness, with a view that can be retained in the oral cavity for up to 12 hours. CHX has been considered an antimicrobial agent is extremely important to present many advantages, among them non-toxic and safety in addition to presenting properties fungicide, algicide and virucidal. The validity of a extemporaneous preparation containing CHX may be estimated at about six months, provided that all the conditions of stability of the active substance, among which stand out the pH of the final product should be between 5.5 and 7.0 avoiding chemical incompatibilities with other components of the solution, and the packing in plastic amber PET. Finally, it is suggested that extemporaneous solutions containing CHX can be effective in treating periodontal diseases, since the conditions for stability, packaging and storage.

  13. Preparação, caracterização e avaliação in vitro de microesferas de bupivacaína em excesso enantiomérico de 50% (S75-R25 Preparación, caracterización y evaluación in vitro de microesferas de bupivacaina en exceso enantiomérico de 50% (S75-R25 Preparation, characterization and in vitro evaluation of 50% enantiomeric excess bupivacaine (S75-R25-loaded microspheres

    Directory of Open Access Journals (Sweden)

    Pedro Paulo Tanaka

    2008-02-01

    Full Text Available JUSTIFICATIVA E OBJETIVOS: As microesferas podem ser utilizadas como um sistema de liberação controlada para prolongar a ação de anestésicos locais. Esse estudo teve como objetivo a preparação, caracterização e análise da liberação in vitro de microesferas de bupivacaína em excesso enantiomérico de 50% (S75-R25. MÉTODO: As micropartículas foram preparadas utilizando o co-polímero de ácido poliláctico-co-glicólico contendo bupivacaína em excesso enantiomérico de 50% pelo método spray-dryed. RESULTADOS: A caracterização das microesferas em relação ao seu tamanho e conteúdo foram similares aos valores teóricos. A liberação in vitro apresentou um padrão bifásico. CONCLUSÕES: O processo de fabricação de microesferas contendo bupivacaína em excesso enantiomérico de 50% pelo método spray-dryed é factível de ser realizado, com resultados semelhantes aos encontrados com microesferas de bupivacaína.JUSTIFICATIVA Y OBJETIVOS: Las micro esferas pueden ser utilizadas como un sistema de liberación controlada para prolongar la acción de anestésicos locales. Este estudio tuvo como objetivo la preparación, caracterización y el análisis de la liberación in vitro de micro esferas de bupivacaina en exceso enantiomérico de 50% (S75-R25. MÉTODO: Las micro partículas fueron preparadas utilizando el copolímero de ácido poliláctico-co-glicólico con bupivacaina en exceso enantiomérico de un 50% por el método spray-dryed. RESULTADOS: La caracterización de las micro esferas con relación a su tamaño y contenido fueron similares a los valores teóricos. La liberación in vitro presentó un estándar bifásico. CONCLUSIONES: El proceso de fabricación de micro esferas con bupivacaina en exceso enantiomérico de 50% por el método spray-dryed se puede realizar con resultados semejantes a los encontrados con micro esferas de bupivacaina.BACKGROUND AND OBJECTIVES: Microspheres can be used as a controlled delivery

  14. Výchova ke zdraví v učebnicích 1. stupně ZŠ: výsledky analýzy didaktického aparátu učebnic prvouky/přírodovědy/Health education in primary school textbooks – results of a content analysis

    Directory of Open Access Journals (Sweden)

    Markéta Hrozová

    2015-02-01

    Full Text Available Cílem empirické studie je prezentace výsledků obsahové analýzy didaktického aparátu učebnic a cvičebnic školního vzdělávacího předmětu prvouka/přírodověda určených pro žáky 1. stupně základních škol z hlediska jejich zaměření na výchovu ke zdraví. V první části studie prezentujeme teoretický základ našeho výzkumu, který spočíval ve vymezení analytických kategorií vyplývajících z holistického pojetí zdraví. V druhé části představujeme cíle a metody výzkumu, výzkumný vzorek sestávající z pěti současných ucelených učebnicových řad učebnic prvouky/ přírodovědy (38 učebnic/cvičebnic; 3136 zkoumaných jednotek, tedy otázek, námětů a úkolů zařazených do učebnic platných pro 1. stupeň základního vzdělávání. Třetí část je věnována výsledkům analýzy, která poukázala na významné rozdíly v prezentaci učiva v oblasti výchova ke zdraví. Z výsledků vyplývá nereflektování holistického pojetí zdraví v jeho plném rozsahu, majoritní zastoupení biologického kontextu zdraví ve zkoumaných učebnicích. Holistické pojetí zdraví nejvíce reflektují učebnice druhých ročníků. Dále poukazujeme na významné rozdíly ve strukturaci učiva v rámci jednotlivých učebnicových řad (absence tematiky zdraví ve většině zkoumaných učebnic 4. ročníků a nestabilitu v distribuci tematických celků zkoumaného vzorku učebnic. V závěru studie (čtvrtá a pátá část jsou diskutovány výsledky, možnosti a limity studie a představujeme eventuality navazujícího výzkumného šetření.

  15. 中国诺贝尔奖获得者心理资本与创新绩效关系--屠呦呦个案剖析%Relationship between Psychological Capital and Innovation Performance of Chinese Nobel Laureate---Based on the study of Tu Youyou

    Institute of Scientific and Technical Information of China (English)

    徐礼平; 李林英

    2016-01-01

    以中国诺贝尔获得者屠呦呦为研究对象,运用扎根理论对官方新闻和专访资料进行深入分析。研究发现,屠呦呦的心理资本包括6个维度:专注、韧性、希望、智慧、责任感、幸福感,这6个维度均与创新绩效呈正相关关系,且其对创新绩效具有积极意义。同时,社会支持系统变量在心理资本与创新绩效的关系中起中介作用。因此应重视个体专注、责任感等积极特征的培养。%Taking Tu Youyou of Nobel Laureate as a case and using the grounded theory to analyze in official news and in‐terview data deeply .We found her psychological capital contains the following six dimensions :concentration ,resilience , hope ,wisdom ,sense of responsibility ,well‐being and six dimensions of psychological capital reflect positive effects on the innovation performance .Besides ,the variables of Social support system had mediating effect on relationship betw een Psy‐chological Capital and Innovation Performance .We suggest that our government should pay more attention to cultivate the positive trait of concentration and sense of responsibility on adolescent students and researchers should combine quantita‐tive with qualitative in the further research .

  16. 论元南戏中的“贪淫破家”悲剧——《小孙屠》解读%Tragedy of Adultery in Southern Ancient Chinese Opera of Yuan Dynasty——A Study on Xiao Sun Tu

    Institute of Scientific and Technical Information of China (English)

    郑传寅

    2001-01-01

    元南戏《小孙屠》是以从良妓女贪淫破家的故事为主要表现对象的悲剧性作品,旨在劝诫世人:妓女性淫,不可娶以为妇。但剧作的意义不仅在此,她揭示了妓女制度对“良人女”心灵的扭曲与毒害,把奸情与官府的昏庸腐败联系起来,概括了丰富的社会生活内容,提升了剧作的思想意义和认识价值。剧中有清官出现,但她与旨在颂扬清官断案的公案剧和清官戏有所不同。《小孙屠》观察生活的视角和评价生活的尺度具有东方特色,反映了东方劳动群众的婚姻家庭观念和社会理想。%Xiao Sun Tu, Southern ancient Chinese opera of Yuan Dynasty, warns people that whores are lewd and are not suitable to become wives by telling us a story of how a married woman ruins her family by unchaste conduct. The play suggests ancient prostitute system distort and poison “gentlewoman's soul”, mirrors the harsh reality and the corruption of officials of Yuan Dynasty. The play contains rich portraits and description of ancient society, which highlight the ideal value.

  17. The Enlightenment of Tu Youyou’s Winning:To Explore the Rule of Tradi-tional Chinese Medicine Treatment and Develop Efficient Drugs%屠呦呦获奖的启示:探索中药治病规律并研发高效药物

    Institute of Scientific and Technical Information of China (English)

    赵丹

    2016-01-01

    屠呦呦获诺贝尔奖激发了人们对中药现代化的热情。文中论述了青蒿素的性质及古人对青蒿治疟记载的矛盾性;复方中药材在水煎提取过程中发生了复杂的化学反应;人体内部的微生物环境及生化反应促进或增强中药成分的疗效;太空育种、遗传工程也能增加药用植物有效成分。不断探索中药治病规律并研发高效药物,是中药现代化的精髓。%Tu Youyou’s winning the Nobel prize inspires the passion of people to modernization of traditional Chinese medicine. This paper explains the properties of artemisinin and the contradictions about Artemisia treatment against malaria in ancient documents; Complex chemical reactions occurred during the extraction of compound Chinese medicinal herbs in hot water; The efficacy of traditional Chinese medicine ingredient is promoted or enhanced for microbiological environment in human internal body and biochemical reaction; Space breeding and genetic engineering can also increase the effective ingredient of pharmaceutical plant. It is the essence of modernization of traditional Chinese medicine to explore the rule of traditional Chinese medicine treatment and develop efficient drugs constantly as a conclusion.

  18. Intra-specific brood parasitism revealed by DNA micro-satellite analyses in a sub-oscine bird, the vermilion flycatcher Parasitismo intraespecífico revelado mediante análisis de microsatélites de ADN en un ave suboscina, el cardenalito o saca tu real

    Directory of Open Access Journals (Sweden)

    ALEJANDRO A RÍOS-CHELÉN

    2008-03-01

    ón extrapareja. En este estudio nos preguntamos si ocurre o no la reproducción extrapareja en el cardenalito o saca tu real (Pyrocephalus rubinus, un suboscino sexualmente dimórfico y socialmente monógamo. Reportamos casos de paternidad extrapareja, maternidad extrapareja y parasitismo intraespecífico, y discutimos nuestros resultados bajo la luz de otros estudios en paserinos

  19. „My tu nie mówimy, ino godumy i jesteśmy z tego dumni”. O tym, dlaczego gwary wielkopolskie przetrwały (na przykładzie języka mieszkańców wsi powiatu międzychodzkiego

    Directory of Open Access Journals (Sweden)

    Bartosz Cemborowski

    2015-12-01

    Full Text Available On why have the dialects of the Province of Greater Poland survived (an example, the language of Międzychodzki district inhabitants The aim of this article is to respond to the question: Why have the dialects of the Province of Greater Poland survived while the dialects from other parts of Poland are in decline? The analytical material relates to the statements of the users of this dialect who live in the western parts of Greater Poland (Międzychodzki district. It was collected during fieldwork in the villages of the Międzychodzki district between 2012 and 2015. The research methodology was based on autobiographical interviews, questionnaires and participant observations. Three important factors which contributed to the survival of the dialects of Greater Poland are: the history of this region, the identity of its people and the fashion for dialect use among the younger generation of Greater Poland’s inhabitants.   „My tu nie mówimy, ino godumy i jesteśmy z tego dumni”. O tym, dlaczego gwary wielkopolskie przetrwały (na przykładzie języka mieszkańców wsi powiatu międzychodzkiego Celem artykułu jest odpowiedź na pytanie, dlaczego gwary wielkopolskie przetrwały, podczas gdy w innych regionach Polski zauważalna jest tendencja do ich zaniku. Materiałem wykorzystanym w analizie są wypowiedzi mieszkańców wsi powiatu międzychodzkiego, którzy posługują się gwarą. Materiał został zebrany podczas badań terenowych w latach 2012–2015 za pomocą takich metod, jak wywiad autobiograficzny, ankiety i obserwacja uczestnicząca. Trzema istotnymi czynnikami, które wpłynęły na to, że gwary przetrwały w języku respondentów, są: historia, identyfikacja i narastająca wśród młodego pokolenia moda na mówienie gwarą.

  20. Low-Amplitude Anisotropic Wave Train Events during Passage of Interplanetary Magnetic Clouds / Mazas Amplitūdas Anizotropiju Viļņu Parādību Secība Starpplanētu Magnētisko Mākoņu Rašanās Laikā

    Science.gov (United States)

    Agarwal, R.; Mishra, R. K.

    2013-04-01

    The work presents a continuation in the series related to the long-term space observations made by ground-based neutron monitoring stations. The cosmic ray intensity variation is considered as affected by interplanetary magnetic clouds during low-amplitude anisotropic wave train (LAAWT) events. It was observed that the solar wind velocity is higher than normal (> 300 km/s) while the interplanetary magnetic field (IMF) strength is lower than normal on the arrival of magnetic cloud during LAAWT events. The proton density is found to remain significantly low at high solar-wind velocity, which was expected. The north/south component of interplanetary magnetic field turns southward one day before the arrival of cloud and remains in this direction after that. The cosmic ray intensity is found to increase with the solar wind velocity. It is noteworthy that the cosmic ray intensity significantly increases before and 90 h after the arrival of such a cloud, and decreases gradually after its passage. The north/south component of IMF (Bz) is found to significantly correlate with latitude angle (Ө) and disturbance storm time index Dst, whereas the geomagnetic activity index (Ap) significantly anti-correlates with these parameters, decreasing with (Ө) and Dst increasing on the arrival of interplanetary magnetic cloud during LAAWT events. Raksts ir turpinājums darbu sērijai par dažādām parādībām kosmosā, kas balstītas uz novērojumiem un datiem, iegūtiem dažādos laika periodos pasaules neitronu monitoringa stacijās (Deep River, Tokija, Maskava, u.c.). Rakstā apskatītās kosmisko staru intensitātes izmaiņas tiek pamatotas ar starpplanētu magnētisko mākoņu parādīšanos. Tiek parādītas saules vēja, magnētiskā lauka spēka, vētru pertubāciju indeksa un citu parametru atkarība no magnētisko mākoņu parādīšanās. Tiek atzīmēts, ka kosmisko staru intensitāte strauji pieaug pirms mākoņu parādīšanās un 90 stundu laikā pēc tiem, un pak

  1. “Local” Reconstruction of Knowledge in the Process of Cross-Cultural Science Communication---A Case Study on the Compilation of HunGai Tong Xian Tu Shuo and Tongwen Suanzhi%跨文化科学传播中知识的“地方性”再建构--以《浑盖通宪图说》与《同文算指》的编译为例

    Institute of Scientific and Technical Information of China (English)

    杨爱东; 宋芝业

    2015-01-01

    科学知识的“地方性”不仅指知识产生、形成以及辩护的“地方性”,还应包括科学知识在跨文化传播中所渗透的诸如社会、文化等因素的“地方性”。李之藻所处的社会文化传统,所持的文化立场以及其自身的知识储备与科学素养都影响到其对《浑盖通宪图说》与《同文算指》的选构与编译。因此,科学的跨文化传播是一个知识在“地方性”因素参与下的再建构过程。这一再建构过程说明“中心—边缘”式科学传播模式是有失偏颇的,同时还反映出儒家文化在学习与接受异质文化中呈现出的某些特性。%The“locality”of scientific knowledge refers not only to the production ,formation ,transfer ,and defense of knowledge , but also the“local” factors that scientific knowledge are penetrated in cross‐cultural communication such as society ,culture etc .. By analyzing that Li Zhizao’s cultural traditions ,cultural position and reserves of know ledge and scientific literacy have an effect on the selection and compilation of HunGai Tong Xian Tu Shuo and Tongwensuanzhi ,this paper reveals the“local”factors of so‐ciety and culture penetrate the process of compiling HunGai Tong Xian Tu Shuo and Tongwensuanzhi ,and elucidates the cross‐cultural communication of scientific knowledge is also a process of reconstruction of local factors participating .This process could prove that the“centre‐edge” model of science communication is biased and this also reflects the characteristic of Confucian culture in the learning w estern culture .

  2. Comportamento de variáveis fisiológicas em atletas de nado sincronizado durante uma sessão de treinamento na fase de preparação para as Olimpíadas de Atenas 2004 Comportamiento de variábles fisiológicas en atletas de nado sincronizado durante una sesion de entrenamiento en la fase de preparación para las Olimpíadas de Atenas 2004 Behavior of physiological variables in synchronized swimming athletes during a training session preparing for the Athens 2004 Olympic Games

    Directory of Open Access Journals (Sweden)

    Marina Guimarães Antunes Pazikas

    2005-12-01

    Full Text Available O objetivo do presente estudo foi avaliar o comportamento de variáveis fisiológicas durante uma sessão de treino de nado sincronizado realizada na fase de preparação para as Olimpíadas de Atenas 2004. MATERIAIS E MÉTODOS: A amostra utilizada foi constituída pelo dueto (24 ± 0 anos representante do Brasil em Atenas 2004. A coleta de dados foi realizada durante uma sessão de treinamento com duração de 198 minutos. O treino se iniciou com a parte física, seguida da parte técnica. Para a determinação da glicemia e da concentração de beta-hidroxibutirato foi utilizado o monitor Optium® com suas respectivas tiras. As concentrações de cortisol e das enzimas lactato desidrogenase (LDH e creatina quinase (CK foram determinadas por radioimunoensaio (DPC© e kits comerciais (CELM®, respectivamente. O acompanhamento da freqüência cardíaca (FC foi realizado com o freqüêncimetro Advantage Polar®. RESULTADOS: Foi observada redução (~2% do peso corporal. A glicemia também apresentou queda (~30% em comparação ao valor obtido no início do treino. Em contrapartida, foi observada elevação na concentração de cortisol (salivar 22% e plasmática 29% e de beta-hidroxibutirato (~340%. Não foi observada alteração significativa na concentração plasmática de CK e de LDH. O acompanhamento da FC demonstrou que dos 198 minutos que constituíram a sessão de treino, 36,5 ± 0,7 minutos foram realizados em intensidade leve; 103,5 ± 0,7 minutos em intensidade moderada, 54,0 ± 2,1 minutos em intensidade alta e 4,0 ± 0,0 minutos em intensidade muito alta. CONCLUSÕES: A perda de peso indica que a reposição hídrica não foi adequada. A redução na glicemia e o aumento na concentração de corpos cetônicos e de cortisol reforçam a importância da suplementação de carboidrato durante o treino de longa duração. O comportamento da FC demonstra que a sessão de treinamento foi realizada em uma intensidade moderada, porém com

  3. Tüütu tegelane helvik / Eva Luigas

    Index Scriptorium Estoniae

    Luigas, Eva

    2015-01-01

    Sageli võib aiakeskustes näha taimepotte, kus mullapinda katab kummaline roheline sammal - nagu plaaditaoline, korrapäraselt harunenud leht oleks tihedalt vastu mulda surutud. Tegemist on hariliku helvikuga (Marchantia polymorpha), rahvas nimetab teda ka maksasamblaks. Koduaeda sattudes võib helvik ruttu levima hakata ja siis on temast lahti saada üsna vaevarikas

  4. Tu eres gallo… pero la de los huevos soy yo

    OpenAIRE

    Pine, Adrienne

    2011-01-01

    En Honduras, la industria maquiladora con sus prácticas disciplinarias y modernas, es vista por muchas personas como una fuerza protectora contra la violencia extrema de las maras y de las calles que es el enfoque principal del discurso diario hondureño. Sin embargo, la industria maquiladora, y las políticas militares y neoliberales (implementados por el fmi y el Banco Mundial) de las cuáles se aprovecha directamente, están estrechamente ligados con las prácticas de violencia asociadas al gén...

  5. Tüütu tegelane helvik / Eva Luigas

    Index Scriptorium Estoniae

    Luigas, Eva

    2015-01-01

    Sageli võib aiakeskustes näha taimepotte, kus mullapinda katab kummaline roheline sammal - nagu plaaditaoline, korrapäraselt harunenud leht oleks tihedalt vastu mulda surutud. Tegemist on hariliku helvikuga (Marchantia polymorpha), rahvas nimetab teda ka maksasamblaks. Koduaeda sattudes võib helvik ruttu levima hakata ja siis on temast lahti saada üsna vaevarikas

  6. COST Action TU1002 - Assessing usability of accessibility instruments

    NARCIS (Netherlands)

    te Brömmelstroet, M.; Silva, C.; Bertolini, L.

    2014-01-01

    The report presents the results of a number of experiential workshops with local planning practitioners. In these workshops, these practitioners, first experienced and then reflected on the usability of accessibility instruments. These workshops were promoted by developers of accessibility instrumen

  7. ¿Qué tan verde es tu mercado?

    Directory of Open Access Journals (Sweden)

    Wilmar R. Olaya-González

    2011-01-01

    Full Text Available El mercado de lo verde y lo ecológico es una tendencia indiscutible en nuestro mundo globalizado, que busca de alguna manera resarcir el daño infringido sobre el medio ambiente. Más allá de simple altruismo, la ecología se ha convertido en una moda, de la cual unos y otros toman ventaja a su mejor acomodo, haciendo del mercado de productos y servicios su tribuna; eco-diseño, planes de responsabilidad social y ambiental, campañas por el reciclaje y consumo de productos "más amigables" con el medio ambiente hacen parte del panorama que se intenta describir en esta reseña. Por medio de un estudio de caso, se hará énfasis en cómo la comunicación asume un papel crucial en la verdadera apropiación del concepto de "consumidor verde" y "consumidor responsable", de manera que la propuesta transversal de las tic se convierta en una herramienta no solo tecnológica, sino también de empoderamiento del papel que la sociedad puede representar en el cuidado de los recursos naturales.

  8. Rule based design workshop at the TU Berlin

    DEFF Research Database (Denmark)

    Tamke, Martin; Palz, Norbert; Josefsson, Kristoffer

    and design concepts to lead into a second workshop further exploring digital design and fabrication tools. The goal is to produce a full-scale prototype to test the ideas, methods and materials. This paper focuses on digital design & fabrication via a pedagogical exploration between academics...

  9. TU-EF-210-02: MRg Hyperthermia

    Energy Technology Data Exchange (ETDEWEB)

    Chopra, R. [UT Southwestern Medical Ctr at Dallas (United States)

    2015-06-15

    The use of therapeutic ultrasound to provide targeted therapy is an active research area that has a broad application scope. The invited talks in this session will address currently implemented strategies and protocols for both hyperthermia and ablation applications using therapeutic ultrasound. The role of both ultrasound and MRI in the monitoring and assessment of these therapies will be explored in both pre-clinical and clinical applications. Katherine Ferrara: High Intensity Focused Ultrasound, Drug Delivery, and Immunotherapy Rajiv Chopra: Translating Localized Doxorubicin Delivery to Pediatric Oncology using MRI-guided HIFU Elisa Konofagou: Real-time Ablation Monitoring and Lesion Quantification using Harmonic Motion Imaging Keyvan Farahani: AAPM Task Groups in Interventional Ultrasound Imaging and Therapy Learning Objectives: Understand the role of ultrasound in localized drug delivery and the effects of immunotherapy when used in conjunction with ultrasound therapy. Understand potential targeted drug delivery clinical applications including pediatric oncology. Understand the technical requirements for performing targeted drug delivery. Understand how radiation-force approaches can be used to both monitor and assess high intensity focused ultrasound ablation therapy. Understand the role of AAPM task groups in ultrasound imaging and therapies. Chopra: Funding from Cancer Prevention and Research Initiative of Texas (CPRIT), Award R1308 Evelyn and M.R. Hudson Foundation; Research Support from Research Contract with Philips Healthcare; COI are Co-founder of FUS Instruments Inc Ferrara: Supported by NIH, UCDavis and California (CIRM and BHCE) Farahani: In-kind research support from Philips Healthcare.

  10. ¡Abre tu cerebro! Descubre la Neurociencia Cognitiva

    Directory of Open Access Journals (Sweden)

    Ángel Correa

    2007-11-01

    Full Text Available En el presente Año de la Ciencia, el gobierno español apuesta por la divulgación científica. Numerosas actividades acercarán a todos los públicos los conocimientos sobre el cerebro y la mente humana desde la Neurociencia Cognitiva.

  11. TU-EF-BRA-00: MR Basics I

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    NMR, and Proton Density MRI of the 1D Patient - Anthony Wolbarst Net Voxel Magnetization, m(x,t). T1-MRI; The MRI Device - Lisa Lemen ‘Classical’ NMR; FID Imaging in 1D via k-Space - Nathan Yanasak Spin-Echo; S-E/Spin Warp in a 2D Slice - Ronald Price Magnetic resonance imaging not only reveals the structural, anatomic details of the body, as does CT, but also it can provide information on the physiological status and pathologies of its tissues, like nuclear medicine. It can display high-quality slice and 3D images of organs and vessels viewed from any perspective, with resolution better than 1 mm. MRI is perhaps most extraordinary and notable for the plethora of ways in which it can create unique forms of image contrast, reflective of fundamentally different biophysical phenomena. As with ultrasound, there is no risk from ionizing radiation to the patient or staff, since no X-rays or radioactive nuclei are involved. Instead, MRI harnesses magnetic fields and radio waves to probe the stable nuclei of the ordinary hydrogen atoms (isolated protons) occurring in water and lipid molecules within and around cells. MRI consists, in essence, of creating spatial maps of the electromagnetic environments around these hydrogen nuclei. Spatial variations in the proton milieus can be related to clinical differences in the biochemical and physiological properties and conditions of the associated tissues. Imaging of proton density (PD), and of the tissue proton spin relaxation times known as T1 and T2, all can reveal important clinical information, but they do so with approaches so dissimilar from one another that each is chosen for only certain clinical situations. T1 and T2 in a voxel are determined by different aspects of the rotations and other motions of the water and lipid molecules involved, as constrained by the local biophysical surroundings within and between its cells – and they, in turn, depend on the type of tissue and its state of health. Three other common applications of MRI exploit its capability to detect and image distinct movements of fluids: MR angiography (MRA), which rivals CT angiography but often requires no contrast medium, monitors the bulk flow of blood; functional MRI (f MRI), distinguishes the perfusion of oxygenated blood from that of de-oxygenated, and lights up parts of the brain that are activated by a stimulus, rather like PET; and diffusion tensor imaging (DTI) indicates the diffusion of free water along tracts of axons, thereby bringing nerve trunks into view. There are variants on all of these themes, and on others as well. Magnetic Resonance Spectroscopy (MRS), for example, can perform non-invasive ‘virtual biopsies’ that allow identification of certain cancers and other lesions. And an MRI-guided needle biopsy can sample brain tissue from a region only millimeters in dimensions. MRI, however, involves deeper and more complex aspects of physics, technology, and biology than do most other imaging modalities, and it is widely considered to be correspondingly more difficult to learn. We could probably cover all of this rather comprehensively if we had 50 hours available rather than 2 ̶ but, to paraphrase a former Secretary of Defense, you tell your story in the time you have allotted. The four presenters and another physicist, Kevin King from GE, have combined their efforts to co-author a single slide show that describes essentials of MRI as simply as possible. It is obviously far from thorough, but hopefully it will succeed in explaining some of the basics in a simplified but still valid fashion; in providing a taste of the numerous capabilities and complexities of the modality; and in whetting your appetite to learn more. Part I. NMR, and Proton Density MRI of the 1D Patient (Wolbarst), begins with an introductory case study that illustrates a half dozen ways in which MRI provides valuable clinical information. It then explores the nuclear magnetic resonance (NMR) phenomenon, which underlies MRI. NMR can be introduced with either of two approaches. In the first, one thinks (loosely) of the nuclei of hydrogen atoms as (rotating and charged and therefore) magnetic objects, whose spin-axes tend to align in a strong external magnetic field, much like a compass needle. As with the Bohr atom, this spin-up/spin-down picture is a highly abridged version of the full quantum mechanical treatment, but still it leads to some useful, legitimate pictures of the NMR process occurring within a voxel: When RF photons of the correct (Larmor) frequency elevate protons in a fixed magnetic field out of their lower-energy spin state into the upper, the NMR phenomenon is indicated by the detectable absorption of RF power. With the addition of a linear gradient field along a multi-voxel, one-dimensional patient/phantom, as well, we can determine the water content of each compartment – an example of a real MRI study, albeit in 1D. Part I concludes with a discussion of the net magnetization at position x, m0(x), under conditions of dynamic thermal equilibrium, which leads into: Part II. Net Voxel Magnetization, m(x,t); T1-MRI; The MRI Device (Lemen), investigates the biophysics of the form of proton spin relaxation process characterized by the time T1. It then moves on to the creation of an MR image that displays the spatial variation in the values of this clinically relevant parameter, again in 1D. Finally, the design and workings of a clinical MRI machine are sketched, in preparation for: Part III. ‘Classical’ NMR; FID Imaging in 1D via k-Space (Yanasak) presents the second standard approach to NMR and MRI, the classical model. It focuses on the time dependence of the net nuclear magnetization, m(x,t), the overall magnetic field generated by the cohort of protons in the voxel at position x. Quite remarkably, this nuclear net magnetization itself acts in a strong magnetic field like a gyroscope in a gravitational field. This tack is better for explaining Free Induction Decay (FID), which involves a brief introduction to the Fourier transform and k-space. This leads to conventional Spin-Echo (S-E) reconstruction techniques for creating clinical images from raw data, and sets the stage for: Part IV. Spin-Echo; S-E / Spin Warp in a 2D Slice (Price) discusses application of the S- E sequence of radiofrequency pulses and gradient magnetic fields to the 1D patient. T2 is introduced but not explained. This Part also considers how to manipulate the image acquisition parameters so as to generate clinical pictures with contrast dominated by spatial variations in PD, T1, or T2. We conclude by demonstrating the spin-warp approach to imaging in 2D with a simple 2×2, 4-voxel example. Much of this material is presented in more detail in the chapter “MRI of the One-dimensional Patient, Part I”, in Advances in Medical Physics, Vol 5 (2014). Copies are on display at the Medical Physics Publishing booth. Learning Objectives: The participant will learn about the processes of NMR and T1 spin relaxation in a tissue voxel in a uniform magnetic field. The participant will learn about combining spin-up/spin-down NMR and T1 processes with a linear gradient to effect frequency-encoding of voxel spatial position. This approach can be used to create proton density and T1 MRI maps, respectively, of the contents of multi-voxel 1D phantoms. The participant will learn about how the ‘classical’ model of NMR it can generate Free Induction Decay (FID) images of 1D phantoms, which involves the use of the Fourier transform in k-space. This can lead simply into standard Spin-Echo images. The participant will learn about extending Spin-Echo imaging into 2 and more dimensions.

  12. Hábeas Corpus (Tendrás tu cuerpo

    Directory of Open Access Journals (Sweden)

    José Domingo Garzón Garzón

    2014-12-01

    Among the living, fiction and observation; from verbal language, image, provocation in the form of spatial intervention, emerged five microstories, worked simultaneity in a polygonal space, which each viewer agreed, in a single representation that stressed the ephemeral nature such as daily, this exercise which, however, it does matter in this writing

  13. Evaluating Credit Risk Management at Agribank Tu Liem

    OpenAIRE

    Pham, Tien

    2015-01-01

    In recent years, bad debts have started to become a huge threat for Vietnamese economic growth. As a result of global economic crisis and unfavorable conditions in Vietnam, the bad debt ratio of the commissioning bank has also become alarming, and the bank has struggled in controlling the credit risk. The aim of this research is to assess the effectiveness of the commissioning bank’s credit risk management based on a comprehensive theoretical framework. Moreover, the readers will learn mo...

  14. TU-CD-213-04: Panel Discussion

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, J. [MD Anderson Cancer Center (United States)

    2015-06-15

    As part of the AAPM’s Scope of Practice, medical physicists are expected to collaborate effectively with practioners and allied health care providers. Interpersonal skills such as communication, negotiation and persuasion are vital for successful collaboration to achieve shared goals. This session will provide some theoretical background of these interpersonal skills as well as specific techniques and practical tools to influence others. Applications of these interpersonal skills for administrative and human resource management purposes vital to medical physicists will be shared. Session attendees will gain knowledge and tools to help them effectively collaborate with administrative and physician leaders in areas such as capital and human resource selection, prioritization, and implementation. Participants will hear methods of how to articulate their goals and to understand the goals of administration, helping ensure alignment of purpose. Session speakers will present one of the topics: equipment selection, budget creation, contracts, and program-related policy development. Specifics may include designing a business case in language that administrators understand, calculating the prioritization of budget requests, and influencing policies for safe and effective care. Human resource topics may include staffing justification, recruitment for fit, employment contracts, and benefits. Speakers will provide examples in both radiation therapy and diagnostic imaging departments and will share experiences and outcomes of their approaches for better results. Learning Objectives: After this course attendees will be better able to Understand the shared goal between administrative and physicist leadership. Articulate the “why” of the technical or human resource need. Utilize communication, negotiation and persuasion tools to improve collaboration.

  15. Videoclips musicales: en un clip, capte tu imagen

    National Research Council Canada - National Science Library

    Fernandez Arismendes, Carolina

    2004-01-01

    Descubrir los estereotipos de genero que muestran los videoclips musicales y las alternativas que existen para construir otras maneras de ser mujer y hombre en la sociedad actual es el objetivo de este trabajo...

  16. TU-A-210-01: HIFU Physics and Delivery

    Energy Technology Data Exchange (ETDEWEB)

    Eames, M. [Focused Ultrasound Foundation (United States)

    2015-06-15

    High-intensity focused ultrasound (HIFU) has developed rapidly in recent years and is used frequently for clinical treatments in Asia and Europe with increasing clinical use and clinical trial activity in the US, making it an important medical technology with which the medical physics community must become familiar. Akin to medical devices that deliver treatments using ionizing radiation, HIFU relies on emitter geometry to non-invasively form a tight focus that can be used to affect diseased tissue while leaving healthy tissue intact. HIFU is unique in that it does not involve the use of ionizing radiation, it causes thermal necrosis in 100% of the treated tissue volume, and it has an immediate treatment effect. However, because it is an application of ultrasound energy, HIFU interacts strongly with tissue interfaces, which makes treatment planning challenging. In order to appreciate the advantages and disadvantages of HIFU as a thermal therapy, it is important to understand the underlying physics of ultrasound tissue interactions. The first lecture in the session will provide an overview of the physics of ultrasound wave propagation; the mechanism for the accumulation of heat in soft-tissue; image-guidance modalities including temperature monitoring; current clinical applications and commercial devices; active clinical trials; alternate mechanisms of action (future of FUS). The second part of the session will compare HIFU to existing ionization radiation techniques. The difficulties in defining a clear concept of absorbed dose for HIFU will be discussed. Some of the technical challenges that HIFU faces will be described, with an emphasis on how the experience of radiation oncology physicists could benefit the field. Learning Objectives: Describe the basic physics and biology of HIFU, including treatment delivery and image guidance techniques. Summarize existing and emerging clinical applications and manufacturers for HIFU. Understand that thermal ablation with HIFU is likely the first of several applications of the technology Learn about some similarities and differences between HIFU and ionizing radiation in terms of physics and biological effects. Learn about some of the technical challenges HIFU faces that might benefit from the experience of radiation oncology physicists including treatment planning improvements, quality assurance procedures, and treatment risk analysis. David Schlesinger receives research support from Elekta Instruments, AB. Matt Eames is an employee of the Focused Ultrasound Foundation which supports research and clinical trials. Dr. Eames conducts research which is supported by the Focused Ultrasound Foundation.

  17. TU-A-210-00: HIFU Therapies - A Primer

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    High-intensity focused ultrasound (HIFU) has developed rapidly in recent years and is used frequently for clinical treatments in Asia and Europe with increasing clinical use and clinical trial activity in the US, making it an important medical technology with which the medical physics community must become familiar. Akin to medical devices that deliver treatments using ionizing radiation, HIFU relies on emitter geometry to non-invasively form a tight focus that can be used to affect diseased tissue while leaving healthy tissue intact. HIFU is unique in that it does not involve the use of ionizing radiation, it causes thermal necrosis in 100% of the treated tissue volume, and it has an immediate treatment effect. However, because it is an application of ultrasound energy, HIFU interacts strongly with tissue interfaces, which makes treatment planning challenging. In order to appreciate the advantages and disadvantages of HIFU as a thermal therapy, it is important to understand the underlying physics of ultrasound tissue interactions. The first lecture in the session will provide an overview of the physics of ultrasound wave propagation; the mechanism for the accumulation of heat in soft-tissue; image-guidance modalities including temperature monitoring; current clinical applications and commercial devices; active clinical trials; alternate mechanisms of action (future of FUS). The second part of the session will compare HIFU to existing ionization radiation techniques. The difficulties in defining a clear concept of absorbed dose for HIFU will be discussed. Some of the technical challenges that HIFU faces will be described, with an emphasis on how the experience of radiation oncology physicists could benefit the field. Learning Objectives: Describe the basic physics and biology of HIFU, including treatment delivery and image guidance techniques. Summarize existing and emerging clinical applications and manufacturers for HIFU. Understand that thermal ablation with HIFU is likely the first of several applications of the technology Learn about some similarities and differences between HIFU and ionizing radiation in terms of physics and biological effects. Learn about some of the technical challenges HIFU faces that might benefit from the experience of radiation oncology physicists including treatment planning improvements, quality assurance procedures, and treatment risk analysis. David Schlesinger receives research support from Elekta Instruments, AB. Matt Eames is an employee of the Focused Ultrasound Foundation which supports research and clinical trials. Dr. Eames conducts research which is supported by the Focused Ultrasound Foundation.

  18. Changing culture - the experience of TU Delft Library

    NARCIS (Netherlands)

    Heijne, M.; Van der Sar, E.

    2004-01-01

    When seeking to introduce change into an organisation, it is usually the organisational structure that is the main focus of attention. A reorganisation, however, does not necessarily resolve underlying cultural problems. It can, in fact, be just these cultural problems that prevent the organisation

  19. TU-F-BRD-01: Biomedical Informatics for Medical Physicists

    Energy Technology Data Exchange (ETDEWEB)

    Phillips, M [University Washington, Seattle, WA (United States); Kalet, I [Kirkland, WA (United States); McNutt, T [Johns Hopkins University, Severna Park, MD (United States); Smith, W [New York Oncology Hematology, Albany, NY (United States)

    2014-06-15

    Biomedical informatics encompasses a very large domain of knowledge and applications. This broad and loosely defined field can make it difficult to navigate. Physicists often are called upon to provide informatics services and/or to take part in projects involving principles of the field. The purpose of the presentations in this symposium is to help medical physicists gain some knowledge about the breadth of the field and how, in the current clinical and research environment, they can participate and contribute. Three talks have been designed to give an overview from the perspective of physicists and to provide a more in-depth discussion in two areas. One of the primary purposes, and the main subject of the first talk, is to help physicists achieve a perspective about the range of the topics and concepts that fall under the heading of 'informatics'. The approach is to de-mystify topics and jargon and to help physicists find resources in the field should they need them. The other talks explore two areas of biomedical informatics in more depth. The goal is to highlight two domains of intense current interest--databases and models--in enough depth into current approaches so that an adequate background for independent inquiry is achieved. These two areas will serve as good examples of how physicists, using informatics principles, can contribute to oncology practice and research. Learning Objectives: To understand how the principles of biomedical informatics are used by medical physicists. To put the relevant informatics concepts in perspective with regard to biomedicine in general. To use clinical database design as an example of biomedical informatics. To provide a solid background into the problems and issues of the design and use of data and databases in radiation oncology. To use modeling in the service of decision support systems as an example of modeling methods and data use. To provide a background into how uncertainty in our data and knowledge can be incorporated into modeling methods.

  20. TU(R)NING WEAKNESS TO STRENGTH MECHANOMUTABLE BIOINSPIRED MATERIALS

    Science.gov (United States)

    2017-04-03

    water at nanoporous two-dimensional interfaces resists high- speed flow through cooperativity," Vol.15(6), pp. 3939-44, 2015 • T. Al-Mulla, Z. Qin, M.J...34Biomateriomics," Materials Genome Initiative - Materials By Design Town Hall, Brown University, Providence, RI March 2012 - “From atoms to structures...Mesoscopic Modeling of Materials (CM4) November 2014 - "Biologically inspired materials & structures for strength and toughness," MIT-CEE Research Speed

  1. Emotions in motion - Tunteet Liikkeessä (TuLi)

    OpenAIRE

    Punkanen, Marko; Saarikallio, Suvi; Luck, Geoff

    2012-01-01

    Depression is a highly prevalent mood disorder which impairs a person’s social skills and quality of life, and affects their ability to recognise and express emotions. Here, we describe the first study to investigate how depression affects expression of emotions perceived in music through spontaneous, expressive body movement. Central to this study is the use of a Dance Movement Therapy (DMT) intervention. Specifically, we investigate how depression and possible co-morbid anxiety ...

  2. TU-F-201-03: Applications in Brachytherapy

    Energy Technology Data Exchange (ETDEWEB)

    Trichter, S. [New York Weill Cornell Medical Ctr (United States)

    2015-06-15

    Since the introduction of radiochromic films (RCF) for radiation dosimetry, the scope of RCF dosimetry has expanded steadily to include many medical applications, such as radiation therapy and diagnostic radiology. The AAPM Task Group (TG) 55 published a report on the recommendations for RCF dosimetry in 1998. As the technology is advancing rapidly, and its routine clinical use is expanding, TG 235 has been formed to provide an update to TG-55 on radiochromic film dosimetry. RCF dosimetry applications in clinical radiotherapy have become even more widespread, expanding from primarily brachytherapy and radiosurgery applications, and gravitating towards (but not limited to) external beam therapy (photon, electron and protons), such as quality assurance for IMRT, VMAT, Tomotherapy, SRS/SRT, and SBRT. In addition, RCF applications now extend to measurements of radiation dose in particle beams and patients undergoing medical exams, especially fluoroscopically guided interventional procedures and CT. The densitometers/scanners used for RCF dosimetry have also evolved from the He-Ne laser scanner to CCD-based scanners, including roller-based scanner, light box-based digital camera, and flatbed color scanner. More recently, multichannel RCF dosimetry introduced a new paradigm for external beam dose QA for its high accuracy and efficiency. This course covers in detail the recent advancements in RCF dosimetry. Learning Objectives: Introduce the paradigm shift on multichannel film dosimetry Outline the procedures to achieve accurate dosimetry with a RCF dosimetry system Provide comprehensive guidelines on RCF dosimetry for various clinical applications One of the speakers has a research agreement from Ashland Inc., the manufacturer of Gafchromic film.

  3. TU-F-201-00: Radiochromic Film Dosimetry Update

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    Since the introduction of radiochromic films (RCF) for radiation dosimetry, the scope of RCF dosimetry has expanded steadily to include many medical applications, such as radiation therapy and diagnostic radiology. The AAPM Task Group (TG) 55 published a report on the recommendations for RCF dosimetry in 1998. As the technology is advancing rapidly, and its routine clinical use is expanding, TG 235 has been formed to provide an update to TG-55 on radiochromic film dosimetry. RCF dosimetry applications in clinical radiotherapy have become even more widespread, expanding from primarily brachytherapy and radiosurgery applications, and gravitating towards (but not limited to) external beam therapy (photon, electron and protons), such as quality assurance for IMRT, VMAT, Tomotherapy, SRS/SRT, and SBRT. In addition, RCF applications now extend to measurements of radiation dose in particle beams and patients undergoing medical exams, especially fluoroscopically guided interventional procedures and CT. The densitometers/scanners used for RCF dosimetry have also evolved from the He-Ne laser scanner to CCD-based scanners, including roller-based scanner, light box-based digital camera, and flatbed color scanner. More recently, multichannel RCF dosimetry introduced a new paradigm for external beam dose QA for its high accuracy and efficiency. This course covers in detail the recent advancements in RCF dosimetry. Learning Objectives: Introduce the paradigm shift on multichannel film dosimetry Outline the procedures to achieve accurate dosimetry with a RCF dosimetry system Provide comprehensive guidelines on RCF dosimetry for various clinical applications One of the speakers has a research agreement from Ashland Inc., the manufacturer of Gafchromic film.

  4. Un chercheur du CERN tué dans un accident

    CERN Multimedia

    2008-01-01

    The accident occured on Sunday between Collonges and Pougnu (Pays de Gex): 4 russians scientists were aboard the vehicle; the driver lost the control of the vehicle and the car made a barrel. (1/4 page)

  5. TU-AB-BRD-04: Development of Quality Management Program

    Energy Technology Data Exchange (ETDEWEB)

    Thomadsen, B. [University of Wisconsin (United States)

    2015-06-15

    Current quality assurance and quality management guidelines provided by various professional organizations are prescriptive in nature, focusing principally on performance characteristics of planning and delivery devices. However, published analyses of events in radiation therapy show that most events are often caused by flaws in clinical processes rather than by device failures. This suggests the need for the development of a quality management program that is based on integrated approaches to process and equipment quality assurance. Industrial engineers have developed various risk assessment tools that are used to identify and eliminate potential failures from a system or a process before a failure impacts a customer. These tools include, but are not limited to, process mapping, failure modes and effects analysis, fault tree analysis. Task Group 100 of the American Association of Physicists in Medicine has developed these tools and used them to formulate an example risk-based quality management program for intensity-modulated radiotherapy. This is a prospective risk assessment approach that analyzes potential error pathways inherent in a clinical process and then ranks them according to relative risk, typically before implementation, followed by the design of a new process or modification of the existing process. Appropriate controls are then put in place to ensure that failures are less likely to occur and, if they do, they will more likely be detected before they propagate through the process, compromising treatment outcome and causing harm to the patient. Such a prospective approach forms the basis of the work of Task Group 100 that has recently been approved by the AAPM. This session will be devoted to a discussion of these tools and practical examples of how these tools can be used in a given radiotherapy clinic to develop a risk based quality management program. Learning Objectives: Learn how to design a process map for a radiotherapy process Learn how to perform failure modes and effects analysis analysis for a given process Learn what fault trees are all about Learn how to design a quality management program based upon the information obtained from process mapping, failure modes and effects analysis and fault tree analysis. Dunscombe: Director, TreatSafely, LLC and Center for the Assessment of Radiological Sciences; Consultant to IAEA and Varian Thomadsen: President, Center for the Assessment of Radiological Sciences Palta: Vice President of the Center for the Assessment of Radiological Sciences.

  6. TU-AB-BRD-02: Failure Modes and Effects Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Huq, M. [University of Pittsburgh Medical Center (United States)

    2015-06-15

    Current quality assurance and quality management guidelines provided by various professional organizations are prescriptive in nature, focusing principally on performance characteristics of planning and delivery devices. However, published analyses of events in radiation therapy show that most events are often caused by flaws in clinical processes rather than by device failures. This suggests the need for the development of a quality management program that is based on integrated approaches to process and equipment quality assurance. Industrial engineers have developed various risk assessment tools that are used to identify and eliminate potential failures from a system or a process before a failure impacts a customer. These tools include, but are not limited to, process mapping, failure modes and effects analysis, fault tree analysis. Task Group 100 of the American Association of Physicists in Medicine has developed these tools and used them to formulate an example risk-based quality management program for intensity-modulated radiotherapy. This is a prospective risk assessment approach that analyzes potential error pathways inherent in a clinical process and then ranks them according to relative risk, typically before implementation, followed by the design of a new process or modification of the existing process. Appropriate controls are then put in place to ensure that failures are less likely to occur and, if they do, they will more likely be detected before they propagate through the process, compromising treatment outcome and causing harm to the patient. Such a prospective approach forms the basis of the work of Task Group 100 that has recently been approved by the AAPM. This session will be devoted to a discussion of these tools and practical examples of how these tools can be used in a given radiotherapy clinic to develop a risk based quality management program. Learning Objectives: Learn how to design a process map for a radiotherapy process Learn how to perform failure modes and effects analysis analysis for a given process Learn what fault trees are all about Learn how to design a quality management program based upon the information obtained from process mapping, failure modes and effects analysis and fault tree analysis. Dunscombe: Director, TreatSafely, LLC and Center for the Assessment of Radiological Sciences; Consultant to IAEA and Varian Thomadsen: President, Center for the Assessment of Radiological Sciences Palta: Vice President of the Center for the Assessment of Radiological Sciences.

  7. TU-CD-BRD-00: Incident Learning / RO-ILS

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    It has long been standard practice in radiation oncology to report internally when a patient’s treatment has not gone as planned and to report events to regulatory agencies when legally required. Most potential errors are caught early and never affect the patient. Quality assurance steps routinely prevent errors from reaching the patient, and these “near misses” are much more frequent than treatment errors. A growing number of radiation oncology facilities have implemented incident learning systems to report and analyze both errors and near misses. Using the term “incident learning” instead of “event reporting” emphasizes the need to use these experiences to change the practice and make future errors less likely and promote an educational, non-punitive environment. There are challenges in making such a system practical and effective. Speakers from institutions of different sizes and practice environments will share their experiences on how to make such a system work and what benefits their clinics have accrued. Questions that will be addressed include: How to create a system that is easy for front line staff to access How to motivate staff to report How to promote the system as positive and educational and not punitive or demeaning How to organize the team for reviewing and responding to reports How to prioritize which reports to discuss in depth How not to dismiss the rest How to identify underlying causes How to design corrective actions and implement change How to develop useful statistics and analysis tools How to coordinate a departmental system with a larger risk management system How to do this without a dedicated quality manager Some speakers’ experience is with in-house systems and some will share experience with the AAPM/ASTRO national Radiation Oncology Incident Learning System (RO-ILS). Reports intended to be of value nationally need to be comprehensible to outsiders; examples of useful reports will be shown. There will be ample time set aside for audience members to contribute to the discussion. Learning Objectives: Learn how to promote the use of an incident learning system in a clinic. Learn how to convert “event reporting” into “incident learning”. See examples of practice changes that have come out of learning systems. Learn how the RO-ILS system can be used as a primary internal learning system. Learn how to create succinct, meaningful reports useful to outside readers. Gary Ezzell chairs the AAPM committee overseeing RO-ILS and has received an honorarium from ASTRO for working on the committee reviewing RO-ILS reports. Derek Brown is a director of http://TreatSafely.org . Brett Miller has previously received travel expenses and an honorarium from Varian. Phillip Beron has nothing to report.

  8. TU-AB-BRD-00: Task Group 100

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    Current quality assurance and quality management guidelines provided by various professional organizations are prescriptive in nature, focusing principally on performance characteristics of planning and delivery devices. However, published analyses of events in radiation therapy show that most events are often caused by flaws in clinical processes rather than by device failures. This suggests the need for the development of a quality management program that is based on integrated approaches to process and equipment quality assurance. Industrial engineers have developed various risk assessment tools that are used to identify and eliminate potential failures from a system or a process before a failure impacts a customer. These tools include, but are not limited to, process mapping, failure modes and effects analysis, fault tree analysis. Task Group 100 of the American Association of Physicists in Medicine has developed these tools and used them to formulate an example risk-based quality management program for intensity-modulated radiotherapy. This is a prospective risk assessment approach that analyzes potential error pathways inherent in a clinical process and then ranks them according to relative risk, typically before implementation, followed by the design of a new process or modification of the existing process. Appropriate controls are then put in place to ensure that failures are less likely to occur and, if they do, they will more likely be detected before they propagate through the process, compromising treatment outcome and causing harm to the patient. Such a prospective approach forms the basis of the work of Task Group 100 that has recently been approved by the AAPM. This session will be devoted to a discussion of these tools and practical examples of how these tools can be used in a given radiotherapy clinic to develop a risk based quality management program. Learning Objectives: Learn how to design a process map for a radiotherapy process Learn how to perform failure modes and effects analysis analysis for a given process Learn what fault trees are all about Learn how to design a quality management program based upon the information obtained from process mapping, failure modes and effects analysis and fault tree analysis. Dunscombe: Director, TreatSafely, LLC and Center for the Assessment of Radiological Sciences; Consultant to IAEA and Varian Thomadsen: President, Center for the Assessment of Radiological Sciences Palta: Vice President of the Center for the Assessment of Radiological Sciences.

  9. TU-AB-BRD-03: Fault Tree Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Dunscombe, P. [University of Calgary (Canada)

    2015-06-15

    Current quality assurance and quality management guidelines provided by various professional organizations are prescriptive in nature, focusing principally on performance characteristics of planning and delivery devices. However, published analyses of events in radiation therapy show that most events are often caused by flaws in clinical processes rather than by device failures. This suggests the need for the development of a quality management program that is based on integrated approaches to process and equipment quality assurance. Industrial engineers have developed various risk assessment tools that are used to identify and eliminate potential failures from a system or a process before a failure impacts a customer. These tools include, but are not limited to, process mapping, failure modes and effects analysis, fault tree analysis. Task Group 100 of the American Association of Physicists in Medicine has developed these tools and used them to formulate an example risk-based quality management program for intensity-modulated radiotherapy. This is a prospective risk assessment approach that analyzes potential error pathways inherent in a clinical process and then ranks them according to relative risk, typically before implementation, followed by the design of a new process or modification of the existing process. Appropriate controls are then put in place to ensure that failures are less likely to occur and, if they do, they will more likely be detected before they propagate through the process, compromising treatment outcome and causing harm to the patient. Such a prospective approach forms the basis of the work of Task Group 100 that has recently been approved by the AAPM. This session will be devoted to a discussion of these tools and practical examples of how these tools can be used in a given radiotherapy clinic to develop a risk based quality management program. Learning Objectives: Learn how to design a process map for a radiotherapy process Learn how to perform failure modes and effects analysis analysis for a given process Learn what fault trees are all about Learn how to design a quality management program based upon the information obtained from process mapping, failure modes and effects analysis and fault tree analysis. Dunscombe: Director, TreatSafely, LLC and Center for the Assessment of Radiological Sciences; Consultant to IAEA and Varian Thomadsen: President, Center for the Assessment of Radiological Sciences Palta: Vice President of the Center for the Assessment of Radiological Sciences.

  10. TU-EF-207-00: Advances in Breast Imaging

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    Breast imaging technology is advancing on several fronts. In digital mammography, the major technological trend has been on optimization of approaches for performing combined mammography and tomosynthesis using the same system. In parallel, photon-counting slot-scan mammography is now in clinical use and more efforts are directed towards further development of this approach for spectral imaging. Spectral imaging refers to simultaneous acquisition of two or more energy-windowed images. Depending on the detector and associated electronics, there are a number of ways this can be accomplished. Spectral mammography using photon-counting detectors can suppress electronic noise and importantly, it enables decomposition of the image into various material compositions of interest facilitating quantitative imaging. Spectral imaging can be particularly important in intravenously injected contrast mammography and eventually tomosynthesis. The various approaches and applications of spectral mammography are discussed. Digital breast tomosynthesis relies on the mechanical movement of the x-ray tube to acquire a number of projections in a predefined arc, typically from 9 to 25 projections over a scan angle of +/−7.5 to 25 degrees depending on the particular system. The mechanical x-ray tube motion requires relatively long acquisition time, typically between 3.7 to 25 seconds depending on the system. Moreover, mechanical scanning may have an effect on the spatial resolution due to internal x-ray filament or external mechanical vibrations. New x-ray source arrays have been developed and they are aimed at replacing the scanned x-ray tube for improved acquisition time and potentially for higher spatial resolution. The potential advantages and challenges of this approach are described. Combination of digital mammography and tomosynthesis in a single system places increased demands on certain functional aspects of the detector and overall performance, particularly in the tomosynthesis mode due to lower photon fluence per projection. This may require fast-frame acquisition and symmetric or asymmetric pixel binning in some systems. Recent studies investigated the performance of increased conversion layer thickness for contrast-enhanced imaging of the breast in dual-energy acquisition mode. In other direct conversion detectors operating in the avalanche mode, sensitivities close to the single photon response are also explored for mammography and breast tomosynthesis. The potential advantages and challenges of this approach are described. Dedicated breast CT brings x-ray imaging of the breast to true tomographic 3D imaging. It can eliminate the tissue superposition problem and does not require physical compression of the breast. Using cone beam geometry and a flat-panel detector, several hundred projections are acquired and reconstructed to near isotropic voxels. Multiplanar reconstruction facilitates viewing the breast volume in any desired orientation. Ongoing clinical studies, the current state-of-the art, and research to advance the technology are described. Learning Objectives: To understand the ongoing developments in x-ray imaging of the breast To understand the approaches and applications of spectral mammography To understand the potential advantages of distributed x-ray source arrays for digital breast tomosynthesis To understand the ongoing developments in detector technology for digital mammography and breast tomosynthesis To understand the current state-of-the-art for dedicated cone-beam breast CT and research to advance the technology. Research collaboration with Koning Corporation.

  11. Si tu devais me dessiner l'Univers

    CERN Document Server

    Saison-Marsollier, Sandrine; Spiro, Michel; Goldberg, Marc

    2015-01-01

    Le CERN a lancé en 2014 un concours de dessins dans les classes de primaire des villes des environs : les enfants étaient invités à s’interroger sur l’Univers, la matière et le métier de chercheur. Le succès de l’opération a été tel qu’il a été décidé d’en faire un livre que l’enseignant pourra parcourir en classe avec ses élèves, discuter avec eux des questions et découvrir ensemble les réponses. Le livre est en effet construit ainsi : sur une double page la question que s’est posée l’enfant et le dessin l’illustrant trouvent leur réponse sur la page d’en face, via un court texte écrit par le chercheur référent (Michel Spiro), qu’accompagne une citation tirée du domaine culturel, destinée à montrer les passerelles entre science, littérature et philosophie. Exemples de questions : Qu’y avait-il avant le Big Bang ? Les chercheurs sont-ils des personnes comme les autres ? Pourquoi les lois de la physique ne changent-elles pas ? Peut-on attraper les particules ?...

  12. ¿Qué tan verde es tu mercado?

    OpenAIRE

    Wilmar R. Olaya-González; Luz Adriana Gómez-Rodríguez

    2011-01-01

    El mercado de lo verde y lo ecológico es una tendencia indiscutible en nuestro mundo globalizado, que busca de alguna manera resarcir el daño infringido sobre el medio ambiente. Más allá de simple altruismo, la ecología se ha convertido en una moda, de la cual unos y otros toman ventaja a su mejor acomodo, haciendo del mercado de productos y servicios su tribuna; eco-diseño, planes de responsabilidad social y ambiental, campañas por el reciclaje y consumo de productos "más amigables" con el m...

  13. Atomic clock with nuclear transition: current status in TU Wien

    OpenAIRE

    Kazakov, G. A.; Schreitl, M.; Winkler, G.; Sterba, J. H.; Steinhauser, G; Schumm, T.

    2011-01-01

    The nucleus of 229Thorium presents a unique isomer state of very low energy and long lifetime, current estimates are around 7.8 eV and seconds to hours respectively. This nuclear transitions therefore is a promising candidate for a novel type of frequency standard and several groups worldwide have set out to investigate this system. Our aim is to construct a "solid state nuclear clock", i.e. a frequency standard where Thorium ions are implanted into Calciumfluoride crystals transparent in vac...

  14. TU Delft y Educación Abierta

    OpenAIRE

    Mulder, Anka

    2015-01-01

    Jornada de Educación Abierta celebrada el 12 de marzo de 2015 en la Universidad Carlos III de Madrid en el marco de la Open Education Week promovida por el Consorcio OpenCourseWare 27 sliders / transparencias

  15. Stratification and Alienation of Chinese Scientists---An Analysis on Tu Youyou's Nobel Prize%中国科学家的分层及异化现象--以屠呦呦获奖为观照点的分析

    Institute of Scientific and Technical Information of China (English)

    钱振华; 崔馨

    2015-01-01

    Stratification in the scientific community is a social phenomenon.It is closely related to the recognition and evalua-tion system of the scientific community, or to the level of scientific reward obtained by scientists.Tu Youyou has no doctoral degree, no overseas background and is not a member of the Chinese Academy.But she made the original scientific work of the first level in the world through long-term research work in China.Then she won the Nobel Prize and got the international rec-ognized.This is called a“civilian” scientist victory.“However, whether“Tu” phenomenon is universal in China?How does a scientist“stand out” from the bottom of the Pyramid to the top in the scientific community?It has important significance to the scientists especially young scholars in China.In the era of big science, the scientists'hierarchical level in the scientific community is directly related with sufficient and effective resources, which is the important or even decisive factor that deter-mines whether the scientists can contribute to the scientific career.%科学共同体中的分层与科学家获得的认可与评价体系是紧密相关的,或者说取决于科学家获得的科学奖励的层次。屠呦呦作为一个没有博士学位,也没有海外留学背景,头上更没有中国两院院士的桂冠的“三无”科学家,却通过长期默默无闻的科研工作,在中国本土做出了具有世界一流水平的原创性工作,并得到国际学界认可。这人被称为“一个‘庶民’科学家的胜利。”[1]然而,“屠呦呦现象”是否具有普遍性?这对中国千千万万的科学家尤其是青年学者来讲,如何“冒尖”,从金字塔的底端上升到顶端具有重要的意义。因为在大科学时代,他们在科学界的分层等级直接关系到能否获得足够有效的资源支持,而这又是决定科学家能否将其才智贡献于科学事业的重要甚至是决定性条件。

  16. Preparação e estabilidade do extrato antociânico das folhas da Acalipha hispida

    Directory of Open Access Journals (Sweden)

    BAILONI Marcio A.

    1998-01-01

    Full Text Available Folhas da Acalipha hispida, foram extraídas com MeOH/ácido cítrico a baixa temperatura e ao abrigo da luz. Os extratos livres de clorofila e outros produtos solúveis em éter etílico foram concentrados por osmose reversa (30°C e finalmente sob vácuo a 35-37°C. Soluções do produto concentrado em tampão citrato/fosfato foram mantidas sob N2 ao abrigo da luz e sob efeito de luz de 2.500 lumens por tempos variáveis acima de 600 h. Pela medida da variação da absorbância dos sistemas a 530 nm foi possível calcular os valores de K e t½ de cada sistema. A principal antocianina foi tentativamente identificada como cianidina-3-arabinosil-glucosídeo.

  17. Determinação de heparina fracionada em preparações farmacêuticas utilizando turbidimetria

    Directory of Open Access Journals (Sweden)

    Samantha Sant'Anna Marotta-Oliveira

    2011-01-01

    Full Text Available A turbidimetric method has been used for quantification of fractionated heparin (FH in pharmaceutical dosage. The UV detection at two wavelengths (290 and 500 nm showed a significant increase in sensitivity of the method, specificity, and linearity to range 5.0-50.0 µg mL-1 and 50.0-200.0 µg mL-1, respectively (r < 0.99. At both wavelengths, the method was precise (inter-assay CV < 5.0%, and intra-assay CV < 3.0%, accurate (maximum deviation of ± 12%, and robust to the parameters evaluated. Turbidimetry proved to be easy, inexpensive and relatively fast. The results obtained attest to the reliability of the method.

  18. Preparação de copolímeros à base de 2-vinilpiridina com propriedades bactericidas

    Directory of Open Access Journals (Sweden)

    Aline S. S. Valle

    2011-01-01

    Full Text Available We report the development of two copolymers based on 2-vinylpyridine, styrene and divinylbenzene (2Vpy-Sty-DVB with different porosity degrees. The copolymers were subsequently quaternized with methyl iodide. To prepare charge transfer complexes, the unmodified copolymers and their derivatives quaternized with methyl iodine were impregnated with iodine. The antibacterial properties of the polymers were evaluated in dilutions ranging from 10² to 10(7 cells/mL of the auxotrophic OHd5-K12 Escherichia coli strain. It was possible to obtain materials with complete antibacterial activity even in the highest cell concentrations tested.

  19. GE Nuclear Hitachi Energy is prepared for the nuclear Renaissance; GE Hitachi Nuclear Energy se prepara para el renacimiento nuclear

    Energy Technology Data Exchange (ETDEWEB)

    Carelli, J. M.

    2008-07-01

    GE Hitachi Nuclear Energy (GEH) is offering two technologies to meet the needs of utilities planning new nuclear projects. An aging workforce, new technological developments and forecasts of considerable new construction projects, raise questions for the entire industry regarding our human resources. In order to prepare for the coming resurgence in new nuclear projects, GEH taking positive action to ensure that adequate human resources are available. From early learning programs that encourage young students to pursue careers in science and technology, to hands-on vocational and engineering programs, GEH works with communities and young people to recruit and train the workforce that will enable our success. (Author)

  20. Preparação e Caracterização de Blendas Híbridas de Poliacrilonitrila e Quitosana

    Directory of Open Access Journals (Sweden)

    Alessandro Cruz Seabra

    2015-12-01

    Full Text Available In this work we report O estudo da interação de íons Fe(II com a polianilina foi feito através da obtenção deste polímero na forma de salthe studies on polymer blend using the polymers polyacrylonitrile (PAN and chitosan (CHI in two compositions weight 56% PAN with 44% of CHI and 88% de PAN with 12% of CHI. We used the techniques of infrared spectroscopy (FT-IR and scanning electron microscopy (MEV for characterizationaplicação de pressão..  The results showed that the polymer blend obtained is stable and effective interaction between PAN and CHI was observed. DOI: http://dx.doi.org/10.17807/orbital.v7i4.778

  1. Atividade vasorrelaxante do 2-nitro-1-fenil-1-propanol em preparaÃÃes vasculares isoladas de ratos

    OpenAIRE

    Teresinha Silva de Brito

    2015-01-01

    2-Nitro-1-fenil-1-propanol (NFP) Ã um nitro-Ãlcool utilizado como intermediÃrio quÃmico na sÃntese de norefedrina e efedrina, agonistas simpatomimÃticos. Sua estrutura quÃmica assemelha-se com a da norefedrina, com substituiÃÃo do grupo NH2 pelo NO2. A presenÃa do grupo nitro nos levou a hipÃtese deste composto ser capaz de produzir efeito vasodilatador, visto que foi demonstrada em estudo prÃvio a aÃÃo vasodilatadora do nitrocomposto 1-nitro-2-feniletano, resultante da estimulaÃÃo da via gua...

  2. Preparação de mulita a partir do mineral topázio Preparation of mullite from topaz

    Directory of Open Access Journals (Sweden)

    R. R. Monteiro

    2004-12-01

    Full Text Available Mulita é o único composto termodinamicamente estável no sistema binário Al2O3 - SiO2, na faixa 70,5 a 74,0% em peso de Al2O3. Mulita metaestável na faixa de 74 a 83,6% de Al2O3, entretanto, pode ser obtida. Devido às suas excelentes propriedades físicas e mecânicas a altas temperaturas, como alto ponto de fusão, baixa expansão térmica, boa resistência à fratura e ao choque térmico, alta resistência à fluência, estabilidade térmica, baixa densidade e baixa constante dielétrica, tem tido um uso cada vez maior em corpos cerâmicos. O mineral mulita, porém, é raro e quase inexistente na natureza. Para atender a um mercado crescente, mulitas sintéticas são produzidas, por meio de misturas de pós de Al2O3 e SiO2 em escala molecular, usando técnica sol-gel, ou por meio da calcinação de minerais que contenham sílica e alumina em suas estruturas, os chamados alumino-silicatos. Normalmente estes minerais contêm impurezas e, muitas vezes, produzem uma mulita acompanhada de uma fase vítrea. Neste trabalho é feito o estudo alternativo para se obter uma mulita pura e de baixo custo por meio da calcinação do topázio Al2SiO4[Fx (OH1-x]2. Topázio incolor e imperial foram utilizados para a produção de mulita. O topázio incolor não tem valor gemológico nem comercial e é abundante na natureza. Para o topázio imperial foram usados refugos provenientes de sua extração. O rendimento da calcinação foi alto, cerca de 80%, obtido a uma temperatura não muito alta, em torno de 1300 ºC, produzindo uma mulita muito pura e sem fase vítrea. A microestrutura da mulita obtida foi do tipo agulhas (whiskers de mulita e apresentou alta porosidade. Essa microestrutura e porosidade são duas propriedades intrínsecas que estão associadas à decomposição do topázio. Este estudo mostrou que o topázio é uma fonte alternativa para a produção de mulita de baixo custo e de alta qualidade.Mullite is the unique intermediate compound thermodynamically stable in the binary system Al2O3 - SiO2, in the weight range from 70.5 to 74.0% of Al2O3. However mullite metastable in the range from 74 to 83.6% of Al2O3 can be obtained. Due to its excellent physical and mechanical properties at high temperatures such as high melting point, low thermal expansion, good resistance to fracture and to thermal shock, high creep resistance, thermal stability, low density and low dielectric constant, it has become widely used in ceramic bodies. However, the mineral mullite is rare and almost nonexistent in the nature. To better assist to a growing market, synthetic mullites are produced, by mixtures of powders of Al2O3 and SiO2 in molecular scale, by using sol-gel technique, or by the calcination of minerals that contains silica and alumina in their structures, so called alumino silicates. Usually these minerals contain impurities, and as consequence, they produce mullite accompanied by a glassy phase. In this work showed that the topaz is another alternative to obtaining a pure and cheap mullite through the calcination of the topaz Al2SiO4 [Fx(OH1-x]2 . Colorless topaz and imperial topaz were used for the mullite production. The colorless topaz doesn't have commercial or gemological value, it is quite abundant. For the imperial topaz it was used drosses of its extraction. The yield in the calcination was high, around 80%, and it was obtained at a temperature not very high, around 1300ºC, producing a very pure mullite without glassy phase. The microstructure of the obtained mullite was of needle type (mulitte whiskers and high porosity. These two features are intrinsic properties that are associated to the topaz decomposition. This study showed that the topaz may be an inexpensive source for obtaining mullite of high quality.

  3. Preço dos alimentos no Brasil: prefira preparações culinárias a alimentos ultraprocessados

    Directory of Open Access Journals (Sweden)

    Rafael Moreira Claro

    2016-01-01

    Full Text Available Resumo: Estudo com objetivo de descrever o preço dos grupos de alimentos consumidos no Brasil considerando a natureza, a extensão e o propósito de seu processamento. Dados provenientes da Pesquisa de Orçamentos Familiares de 2008-2009 foram utilizados. O preço médio dos grupos (in natura, ingredientes culinários, processados e ultraprocessados e seus respectivos subgrupos de alimentos foram estimados para o Brasil segundo renda, região e área. Os produtos in natura e ingredientes culinários apresentaram menor preço por caloria quando comparado aos demais grupos, sugerindo uma vantagem econômica no preparo de refeições no lar em comparação a sua substituição por ultraprocessados. As famílias de maior nível econômico pagaram preço mais elevado por suas aquisições, enquanto as regiões Nordeste, Norte e a área rural pagaram os menores preços. Enquanto alimentos frescos (como carnes, leite, frutas e hortaliças tendem a custar mais caro que alimentos ultraprocessados, grãos secos (como o arroz e o feijão despontam como uma alternativa mais econômica para adoção de práticas alimentares saudáveis.

  4. "Preparação de anfifilicos potencialmente geradores de mesomorfismos e estudo de suas propriedades fisico-quimicas"

    OpenAIRE

    Barbosa, Alberto Aparecido

    1995-01-01

    Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciencias Fisicas e Matematicas Com o objetivo de estudar comparativamente propriedades físico-químicas de sistemas liotrópicos, elaborados com anfifílicos de estruturas similares, fixou-se a cadeia alquílica (n-decil) e variou-se a parte polar (PO4=, PO3= e SO3-). O anfifílico escolhido como referência, devido a sua sólida base de investigação experimental em liomesofases, foi o n-decil sulfato de sódio (SDS). Após a prep...

  5. Pre-modern Knowledge System in the Acculturation between Chinese and Western Culture: a Case Study of Sancai yiguan tu (The Map of the Coherence of the World)) Preserved in Library of Congress%中西文化调适中的前近代知识系统——美国国会图书馆藏《三才一贯图》研究

    Institute of Scientific and Technical Information of China (English)

    欧阳楠

    2012-01-01

    The current study is focused on a hybrid-style 18th-centurty map named Sancai yiguan tu 三才一贯图 (The Map of the Coherence of the World), a drawing composed of over ten separate sub-maps, compiled by Lü Fu lived in mid-Qing Dynasty. The prolegomenon and two major sub-maps (the Sub-map of Earth and Heaven Map and the Star Chart of North and South Hemispheres) are especially indicative of the impact brought by the foreign missionaries and the consequent cultural amalgamation with the intention to conciliate between the traditional knowledge system and the western one. By tracing the sources he used and consulted, we can pinpoint that among an array of works, Ferdinand Verbiest’s maps exerted the greatest influence on the two major sub-maps. Furthermore, by analyzing those choices he made to fulfill his goal to present the inner coherence of the world, the process of how traditional Chinese literati absorbed and transformed western knowledge on their own initiative with great craft can be revealed. Lastly, the concept of Tu 图(graph, chart or map) in the pre-modern Chinese context can be further discussed, by doing so, a new horizon of the studies of traditional cartography can be appreciated by breaking through the disciplinary limitation caused by the division of modern knowledge system.%本文研究的是康熙年间吕抚所绘《三才一贯图》,探讨该图受到西方传教士影响较深的《天地全图》、《南北二极星图》这两幅分图及图序的内容。重点在于梳理两幅分图的知识系统,拟构它们与明末清初时期在华西方传教士所绘诸种地理图与天文图之间的关系,得出结论认为吕抚受南怀仁图的影响最大。此外,结合全图其它表现中国传统知识的内容,讨论中西两种知识体系如何在普通士人中实现榫接和重构。最后,从吕抚这幅图的丰富内容注意到"图"在历史时期的多样性,提出地图史研究应拓宽视野,打破现代学科界限的观点。

  6. Flow, simulation and dynamics. Including a contribution of Vienna University (TU Wien); Stroemung, Simulation und Dynamik. Mit einem Beitrag der TU Wien

    Energy Technology Data Exchange (ETDEWEB)

    Kauder, K. [ed.

    1998-12-31

    This report covers the following topics: Gas-flow through clearances in screw vacuum pumps - part 1, gas flow research at a plane screw type machine model - part 2, two-phase screw-type engine - problems of the filling process, oil as a design parameter in screw-type engines - use of non-newtonian oils, experimental determination of the dynamic properties of screw compressors, hot gas screw-type engine - simulations as a basis for construction - part 3. [Deutsch] Dieser Bericht hat folgende Inhalte: Gasspaltstroemungen in Schraubenspindel-Vakuumpumpen - Teil 1, Gasspaltstroemungen in einem ebenen Schraubenmaschinenmodell - Teil 2, Zweiphasen-Schraubenmotor-Probleme des Fuellungsvorganges, Oel als Konstruktionselement - Einsatz nicht-newtonscher Oele, die experimentelle Bestimmung der dynamischen Eigenschaften von Schraubenkompressoren, die Heissgasschraubenmaschine - simulationsgestuetzte Auslegung - Teil 3

  7. Concepção pedagógica do curso psicoprofilático de preparação para o nascimento

    OpenAIRE

    Frias, Ana

    2014-01-01

    The Courses to Psicoprophylactic Preparation for the Birth (PPB) are not limited to the prevention of pain in childbirth. They have other purposes: to reduce anxiety; to control stress; to promote self-esteem and satisfaction and to promote feelings of ability and postpartum adjustment. Objectives: 1) to reflect on the pedagogical conception of the course to preparation for birth; 2) to know the relationship of PPB with maternal/newborn (NB) benefits. Methodology: cross-sectional, quantitativ...

  8. Preparação de derivados triptamínicos e dímeros inibidores de acetilcolinesterase a partir da bufotenina isolada de Anadenanthera (Fabaceae: Mimosideae)

    OpenAIRE

    Moreira, Leandro Andrade

    2016-01-01

    O alcaloide indólicobufotenina (5-hidróxi-N,N-dimetiltriptamina) tem sido encontrado em diversas fontes vegetais e animais e, recentemente, vem despertando grande atenção desde a sua detecção em vários fluídos corporais humanos. Esse trabalho descreve um protocolo eficiente para o isolamento da bufotenina de sementes de espécies de Anadenanthera (A. peregrina eA. colubrina), árvores amplamente distribuídas no cerrado brasileiro, e descreve o uso deste alcaloide como matéria-prima apropriada p...

  9. Preparação e estabilização de uma suspensão cerâmica de porcelana de ossos.

    OpenAIRE

    Gerardo Augusto Vera Alatrista

    2008-01-01

    O estudo da dispersão e estabilização de barbotinas de porcelana de cinza de ossos é uma etapa muito importante no processo de conformação de peças cerâmicas, assim como também a etapa de obtenção da sua principal matéria-prima que é a cinza de ossos, devido a que constitui 50% em peso da porcelana e por ser uma matéria-prima renovável, reciclável e com alto valor agregado na fabricação de porcelana. Neste trabalho se investigou a influência das temperaturas de calcinação dos ossos (700, 800,...

  10. Nanopartículas de poli-ε-caprolactona carregadas com hidrocortisona: preparação usando planejamento fatorial e sua avaliação

    Directory of Open Access Journals (Sweden)

    Nayara Alonso Cazo

    2012-07-01

    Full Text Available Polymeric-nanoparticle systems such as nanocapsules and nanospheres have a great potential in applications for nanoencapsulation of corticosteroids which show low solubility in water. The physicochemical characteristics of nanoparticle suspensions are important pre-requisites for the successful development of new dosage form. In this study, hydrocortisone-loaded poly-e-caprolactone nanoparticles have been prepared by the interfacial deposition method. A 3-factor 2-level factorial design was used to study and optimize nanoparticles formulation. This factorial design was used to study the contrasts and effects of independent variables on particle size distribution, morphology, surface charge, drug content, entrapment efficiency and in vitro drug release profiles. The screened independent variables were: the concentration of hydrocortisone, poly-e-caprolactone and isodecyl oleate. A High Performance Liquid Chromatography method was developed and validated for hydrocortisone quantification. Special attention was given to both absolute recovery and entrapment efficiency. The results of optimized formulations showed a narrow size distribution with a polydispersity index near to 0.200. The particle sizes were on average 109.2 and 236.5nm to nanospheres and nanocapsules, respectively. In the best formulations the zeta potential was higher than 30 mV (in module and the absolute recovery and entrapment efficiency were higher 82% and nearly 60%, respectively. The main variables were the quantity of the polymer and of the oil. Nanoparticles observed by the Scanning Electron Microscope depicted extremely spherical shape. In vitro release studies were performed through dialysis with continuous stream. Nanocapsules and nanospheres showed a similar pure diffusion release mechanism according to Korsmeyer-Peppas’s model.

  11. Preparação e caracterização de filmes biodegradáveis a partir de blendas de amido com polietileno

    OpenAIRE

    Bona, Jaqueline Ceolin de

    2007-01-01

    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de Alimentos. Os plásticos sintéticos são utilizados em nosso cotidiano, principalmente devido a seu baixo custo, fácil processamento, elevada aplicabilidade e durabilidade, sendo esta última característica o ponto de maior desvantagem da sua aplicação na fabricação de embalagens descartáveis para alimentos. O crescente acúmulo de lixo não biodegradável, aliado à dif...

  12. Filmes poliméricos ultrafinos produzidos pela técnica de automontagem: preparação, propriedades e aplicações

    Directory of Open Access Journals (Sweden)

    Paterno Leonardo Giordano

    2001-01-01

    Full Text Available The self-assembly technique is a powerful tool to fabricate ultrathin films from organic compounds aiming at technological applications in molecular electronics. This relatively new approach allows molecularly flat films to be obtained on a simple and cheap fashion from various types of material, including polyelectrolytes, conducting polymers, dyes and proteins. The resulting multilayer films may be fabricated according to specific requirements since their structural and physical properties may be controlled at the molecular level. In this review we shall comment upon the evolution of preparation methods for ultrathin films, the process of adsorption and their main properties, as well as some examples of technological applications of layer-by-layer or self-assembled films.

  13. O uso de recursos virtuais na preparação pré-operatória de aneurismas infrarrenais: explorando o potencial do OsiriX

    Directory of Open Access Journals (Sweden)

    Giovani Jose Dal Poggetto Molinari

    2014-04-01

    Full Text Available Introdução: Desde os últimos anos, tem crescido o interesse dos cirurgiões vasculares com prática em cirurgia endovascular na utilização de softwares de manipulação de imagens tomográficas, principalmente quando se refere à sua utilização no reparo endovascular dos aneurismas de aorta abdominal infrarrenais. Assim, o pós-processamento das imagens tornou-se uma grande ferramenta na interpretação e documentação das alterações, melhorando a produtividade e a precisão das informações, utilizando volumes cada vez menores de contraste iodado no planejamento e execução do tratamento endovascular. Da mesma forma, menor é a exposição à radiação ionizante no intraoperatório. Divulgam-se os resultados iniciais da análise da viabilidade da manipulação de imagens tomográficas no software OsiriX por meio da fluoroscopia virtual. Métodos: Através da manipulação de imagens de cortes tomográficos finos sob-reconstrução tridimensional por volume, foi possível manipular valores de projeção da distribuição de dose irradiada. A esta configuração, foi atribuído o nome de Virtual Fluoroscopy, formato reprodutível em qualquer plataforma OsiriX. Com isto, obteve-se uma imagem biplanar aparentemente a uma fluoroscopia operatória do doente. Quando comparadas à angiografia e fluoroscopia intraoperatória, estas imagens revelaram-se equivalentes. DISCUSSÃO: A manipulação de dados de distribuição da dose irradiada em uma superfície permite que se visualizem como opacas áreas de alto contraste (como superfícies ósseas e como transparentes valores de baixa atenuação (partes moles. Orientados por marcações nas artérias renais, pode-se prever minuciosamente o seu posicionamento anatômico em relação à sua visualização sob fluoroscopia. Outrossim, a antecipação do correto posicionamento do aparelho de radioscopia com o uso desta técnica permite a obtenção da imagem com o mínimo de interferência do efeito parallax. Com isso, acreditamos ser possível estimar o posicionamento topográfico das artérias renais em imagem bidimensional intraoperatória. Consegue-se reduzir o número de angiografias na tentativa de se obter a melhor imagem que forneça a localização das artérias renais e do colo do aneurisma, reduzindo sobrecarga renal em pacientes vulneráveis.

  14. Desenvolvimento de metodologias analíticas para determinação de antibióticos em preparações farmacêuticas e leite

    OpenAIRE

    Kêlia Barbosa Freitas, Sueny

    2011-01-01

    Os antibióticos são substâncias químicas que combatem ou inibem o crescimento de organismos, sendo muito utilizados para o controle de certas doenças de origem bacteriana. Faz-se necessário atestar a qualidade destes produtos, uma vez que se torna cada vez mais crescente o seu uso. Além disso, resíduos de antibióticos podem permanecer em alimentos de origem animal, acima de valores considerados seguros quando não são respeitadas as boas práticas veterinárias. Neste trabalho ...

  15. DEL EDITOR. ¿CÓMO SE PREPARA EL VALLE DE ABURRÁ PARA ENFRENTAR EL CAMBIO CLIMÁTICO? PROPUESTAS DESDE EL PLAN BIO 2030

    Directory of Open Access Journals (Sweden)

    Nora Cadavid

    2013-01-01

    Full Text Available La planifi cación urbana de largo plazo debe incluir necesariamente estrategias para enfrentar las nuevas condiciones que el cambio climático generaría, con períodos de lluvia y sequía más intensos y prolongados. De la misma manera, es necesario adoptar medidas para reducir las emisiones de gases con efecto invernadero derivados de la dinámica urbana. El siguiente artículo describe las estrategias que el plan BIO 2030 propone para mitigar el impacto del Área Metropolitana del Valle de Aburrá sobre el cambio climático global, y al mismo tiempo, adaptarse a las posibles nuevas condiciones de precipitación en el ámbito regional y local. El texto describe propuestas sobre diversos temas, tales como el abastecimiento hídrico, el drenaje urbano sostenible, el fortalecimiento de las funciones ambientales del espacio público, el incremento de la oferta de áreas protegidas urbanas y periurbanas, el mejor tratamiento de los residuos, así como medidas para el mejoramiento de la efi ciencia energética relacionadas con una movilidad más limpia y una distribución de actividades urbanas mejor articulada con la infraestructura de transporte.

  16. During Her Pregnancy, Kathy Prepares for Breastfeeding. Mother-to-Mother Support = Durante Su Embarazo, Josefa Se Prepara para la Lactancia. Apoyo Madre a Madre.

    Science.gov (United States)

    Magalhaes, Rebecca; Stone-Jimerez, Maryanne; Allen de Smith, Paulina; Smith, Natalia

    These magazine-sized booklets, one in English, one in Spanish, are in a cartoon format and designed to be used by people with limited literacy in English or Spanish. They explain the importance of both prenatal care and breastfeeding. (Adjunct ERIC Clearinghouse for ESL Literacy Education) (KFT)

  17. Preparação e caracterização de membranas cerâmicas de cordierita Preparation and characterization of cordierite ceramic membranes

    Directory of Open Access Journals (Sweden)

    F. A. Silva

    2006-12-01

    Full Text Available As membranas cerâmicas encontram larga aplicação, principalmente em processos cujas temperaturas de trabalho são superiores a 250 ºC, como também na separação de soluções em que o pH é extremamente ácido, ou mesmo quando há solventes orgânicos no sistema. Em contrapartida, as membranas cerâmicas apresentam a desvantagem de apresentar um alto custo de fabricação, principalmente em relação às matérias-primas que são geralmente sintéticas (zirconia, alumina, titânia e sílica. Atualmente, o principal foco de preocupação no desenvolvimento destas membranas é otimizar os custos de produção, encontrando matérias-primas naturais a preços mais competitivos, além de processos de produção mais eficientes, como a extrusão. A produção de membranas por extrusão permite sua utilização em processos de fluxo tangencial, possibilitando a sua aplicação em microfiltração. Este trabalho consistiu na confecção de membranas tubulares de cordierita obtidas a partir de matérias-primas naturais, tais como argilas e talco, e pelo processo de extrusão. Foram utilizadas quatro temperaturas de sinterização (1150, 1200, 1250 e 1280 ºC, objetivando mostrar a influência destas temperaturas nas características das membranas. Foi feito o ensaio de difração de raios X na massa após a sinterização, e o resultado mostrou a formação da cordierita nas quatro sinterizações efetuadas. Com relação às dimensões e a distribuição dos poros das membranas, os resultados de microscopia eletrônica de varredura e de porosimetria através de intrusão de mercúrio mostraram poros com tamanhos de 1,4, 2,2, 3,3 e 4,1 µm e porosidade de 28,7, 29,1, 27,7 e 24,3%, para as temperaturas de sinterização 1150, 1200, 1250 e 1280 ºC, respectivamente, caracterizando-as como membranas para microfiltração. Os ensaios de permeabilidade através de aplicação de fluxo tangencial com água destilada mostraram que as membranas sinterizadas a 1280 ºC obtiveram maior permeabilidade, seguindo-se das de 1250 ºC, 1200 ºC e as de 1150 ºC. Os valores médios dos fluxos encontrados nas membranas sinterizadas nas temperaturas de 1150, 1200, 1250 e 1280 ºC foram de aproximadamente 68, 143, 378 e 587 kg/h.m², respectivamente.Membrane separation processes find large applications. Ceramic membranes are applied in several processes, mainly in application above 250 ºC, as well as in separation of solutions with pH extremely acid and even in systems with organic solvents. On the other hand, ceramic membranes show high cost of fabrication, mainly in relation to the raw synthetic materials (zirconia, alumina, titania and silica. Therefore, the main concern in the development of these membranes is to optimize the cost using natural non-expensive raw materials and more efficient ceramic processing, such as extrusion. The fabrication of ceramic membranes by extrusion gives the possibility to use cross flow system, which is very useful in microfiltration and ultrafiltration separation processes. The aim of this work is to prepare tubular cordierite membranes from raw materials such as clays and talc and by extrusion processing. Four sintering temperatures (1150, 1200, 1250 and 1280 ºC were used to show the effect on the morphological characteristics of the membranes. The membranes were characterized by X-ray diffraction, scanning electron microscopy and mercury intrusion porosimetry. The results showed the formation of cordierite phase at all sintering temperatures. The membranes presented pore size of 1.4, 2.2, 3.3 and 4.1 µm and porosity content of 28.7, 29.1, 27.7 and 24.3% for sintering temperaturesf 1150, 1200, 1250 and 1280 ºC, respectively. These values show that these membranes are suitable to be applied in microfiltration separation processes. The results of water flux, at steady state, show that the membrane sintered at 1280 ºC presented the highest value, 587.3 kg/m².h, followed by 377.7 kg/m².h (1250 ºC, 143.4 kg/m².h (1200 ºC and 67.8 kg/m².h (1150 ºC.

  18. During Her Pregnancy, Kathy Prepares for Breastfeeding. Mother-to-Mother Support = Durante Su Embarazo, Josefa Se Prepara para la Lactancia. Apoyo Madre a Madre.

    Science.gov (United States)

    Magalhaes, Rebecca; Stone-Jimerez, Maryanne; Allen de Smith, Paulina; Smith, Natalia

    These magazine-sized booklets, one in English, one in Spanish, are in a cartoon format and designed to be used by people with limited literacy in English or Spanish. They explain the importance of both prenatal care and breastfeeding. (Adjunct ERIC Clearinghouse for ESL Literacy Education) (KFT)

  19. Métodos de preparação e atividade biológica do ácido quinolínico e derivados

    Directory of Open Access Journals (Sweden)

    Souza Marcus Vinícius Nora de

    2003-01-01

    Full Text Available In 1981 2,3-pyridine dicarboxylic acid (quinolinic acid was discovery to be a selective agonist for the N-methyl -D-aspartic acid (NMDA receptor. As a consequence it possesses neurotoxic activity resulting from overstimulation of the receptor. Quinolinic acid is implicated as an etiological factor in a range of neurodegenerative disease including AIDS related dementia, Huntington´s disease and Lyme disease. In the design of novel therapies to treat these diseases, some molecules have been identified as an important target. In this paper we described different methods to prepare quinolinic acid and derivatives.

  20. PreparaÃÂÃÂo e CaracterizaÃÂÃÂo de Compostos Fe-Al-O pelo processo Sol- Gel

    OpenAIRE

    Marcia Ferreira Bini

    2009-01-01

    Este trabalho tem como proposta central a sÃÂntese e a caracterizaÃÂÃÂo de materiais ÃÂxidos mistos policristalinos (sistema (Fe-Al-O)), de escala nanomÃÂtrica (pÃÂs nanomÃÂtricos). Dentre os possÃÂveis compostos deste sistema, a hematita ( -Fe2O3), que ÃÂ isomorfa a alumina ( -Al2O3) apresenta propriedades magnÃÂticas interessantes. AlÃÂm disso, a fase FeAlO3 apresenta comportamento multiferrÃÂico magnetoelÃÂtrico. Os estudos foram conduzidos com o intuito de entender e explorar as modificaÃ...

  1. Preparação e caracterização de revestimentos ópticos multicamada para aplicações em janelas inteligentes

    OpenAIRE

    Cui, Hai-Ning

    2005-01-01

    Tese de doutoramento em Ciências. Multilayer films with a tungsten oxide (WO3) layer were deposited by reactive dc magnetron sputtering onto glass substrates for electrochromic (EC) applications. The configuration of the smart EC devices (ECDs) or window is ITO (Indium-tin-oxide)/WO3/Li+electrolyte/counter electrode film/ITO. Depending on the choice of different counter electrodes such as SnO2, V2O5, ZrO2 and the doped Mo (or Fe) films, a total of 15 different window structures were fabric...

  2. A fase de preparação para a escrita nas aulas de iniciação de Inglês e de Espanhol

    OpenAIRE

    Oliveira, Cláudia Sofia Marques

    2013-01-01

    Relatório de estágio do Mestrado em Ensino de Inglês e de Espanhol/Alemão/Francês no Ensino Básico e no 3º Ciclo do Ensino Básico e no Ensino Secundário, apresentado à Faculdade de Letras da Universidade de Coimbra

  3. Preparação de pigmentos de aluminatos de cobalto para aplicação em vidrados cerâmicos

    Directory of Open Access Journals (Sweden)

    A. F. Costa

    Full Text Available Resumo Os pigmentos inorgânicos são os mais utilizados industrialmente, pois apresentam maior estabilidade química e térmica e podem ser produzidos com um elevado grau de pureza e uniformidade. Essas características levaram ao desenvolvimento de diferentes cores intensas, estáveis e adequadas ao uso como pigmentos. O presente trabalho tem por objetivo sintetizar materiais cerâmicos com estrutura espinélio para uso como pigmento cerâmico e aplicá-los em fritas cerâmicas. O processo de síntese foi realizado pela rota da gelatina e com os pós obtidos foram conduzidos um estudo de evolução de fases cristalinas por meio de difração de raios X, refinamento das estruturas por meio do método Rietveld, espectroscopia na região do infravermelho, microscopia eletrônica de varredura, espectroscopia na região do UV-visível e colorimetria. Foram observadas nos difratogramas reflexões características da estrutura espinélio. O tamanho médio de cristalito e a cristalinidade aumentou com o aumento da temperatura de calcinação. A coloração dos pigmentos variou do azul claro ao violeta azul.

  4. Preparação e caracterização de resina epóxi transparente dopada com nanoestruturas semicondutoras de CdS

    OpenAIRE

    Silveira, João Borges da [UNESP

    2009-01-01

    O presente trabalho visou a produção e o estudo das propriedades físicas de amostras de resina epóxi transparente dopadas com diferentes concentrações de nanocristais de sulfeto de cádmio (CdS). As técnicas de difração de raios-X, espectroscopia no infravermelho, espectroscopia Raman e calorimetria diferencial de varredura (DSC) foram utilizadas para estudar as propriedades estruturais. Espectroscopias de refletância e de transmitância foram utilizadas para obter a dispersão do índice de refr...

  5. Filmes poliméricos baseados em amido e lignossulfonatos: preparação, propriedades e avaliação da biodegradação

    Directory of Open Access Journals (Sweden)

    Marina Rodrigues Campagner

    2014-12-01

    Full Text Available Este trabalho envolveu a obtenção de filmes poliméricos a partir de amido de milho e incorporação de lignina na forma de lignossulfonato. A motivação deste trabalho está relacionada à utilização do amido na obtenção de filmes devido ao seu potencial de biodegradação, além do aproveitamento de resíduos de lignossulfonatos provenientes da indústria de celulose e papel. Para a obtenção dos filmes foram utilizados lignossulfonatos em pó e foram realizadas diversas moldagens com variação do tipo e da proporção (1% a 4% de lignossulfonato utilizado. Algumas análises foram realizadas nos filmes poliméricos para comparar propriedades térmicas por DSC (Calorimetria Exploratória Diferencial, e propriedades de tração, em equipamento de DMTA (Análise Térmica Dinâmico-Mecânica. Neste estudo são destacadas as análises da biodegradação e da emissão dos gases liberados utilizando a técnica de Cromatografia Gasosa. De acordo com os resultados de DMTA, a maioria dos filmes poliméricos contendo lignossulfonatos apresentou resistência à tração inferior quando comparados aos filmes poliméricos sem lignossulfonatos (1,024 MPa. Porém, os filmes contendo lignossulfonato modificado com sódio (Vixil S apresentaram maiores valores de resistência à tração quando comparado aos outros filmes contendo os lignossulfonatos utilizados (Vixil I e Vixil Tan. Nas análises térmicas foram notadas semelhanças entre os dois tipos principais de filmes poliméricos (amido; e amido com lignossulfonato, com alguns deslocamentos das temperaturas dos principais picos máximos dos filmes contendo lignossulfonato (4%, Vixil S. Na análise de biodegradabilidade foi verificado que os materiais são biodegradáveis e houve uma emissão maior de CO2 e N2O das amostras dos filmes contendo 4% lignossulfonato do tipo Vixil S, em comparação com as amostras sem lignossulfonato.

  6. Preparação e caracterização de pós cerâmicos de aluminatos de lítio

    Directory of Open Access Journals (Sweden)

    Ribeiro Roberto Ananias

    2001-01-01

    Full Text Available The preparation of gamma-LiAlO2 by coprecipitation and sol-gel synthesis was investigated. Ceramic powders obtained by coprecipitation synthesis were prepared from aqueous solutions of aluminum and lithium nitrates using sodium hydroxide as precipitant agent. By sol-gel synthesis, the ceramic powders were prepared from hydrolysis of aluminum isopropoxide. The materials obtained by two routes of synthesis were dried at 80ºC and calcined at 550, 750, 950 and 1150ºC. The characterization was done by X-ray diffraction, infrared spectroscopy, emission and absorption atomic spectrometry, helium picnometry, specific surface area (BET method and scanning electronic microscopy. Mixtures of crystalline phases were obtained by coprecipitation synthesis: 80ºC- LiAl2(OH7.2H2O + Al(OH3; 550 and 750ºC- alpha-LiAlO2 + eta-Al2O3; 950 and 1150ºC- gamma-LiAlO2 + LiAl5O8. Chemical analysis showed molar ration Al/Li @ 3. Crystalline single-phases were obtained by sol-gel synthesis above 550ºC: 550ºC-alpha-LiAlO2; 750, 950 and 1150ºC-gamma-LiAlO2. These powders presented molar ration Al/Li @ 1. Thus, gamma-LiAlO2 crystalline phase was obtained at 750ºC by sol-gel synthesis while by coprecipitation synthesis, a mixture of crystalline phases was obtained. These results showed the superiority of the sol-gel synthesis for the preparation of pure gamma-LiAlO2.

  7. Preparação e caracterização de poliuretanos contendo diferentes quantidades de óleo de baru

    Directory of Open Access Journals (Sweden)

    Elizabeth Luiza de Almeida

    2016-01-01

    Full Text Available Resumo O óleo de baru (OB é uma matéria-prima renovável proveniente do cerrado brasileiro que possui elevado percentual de insaturação. O objetivo principal do trabalho foi o estudo das espumas de poliuretano com diferentes quantidade de óleo de baru saponificado. Os espectros de FTIR mostraram bandas representativas do poliol, óleo de baru e do pré-polímero. As curvas TG-DSC nas atmosferas de ar e N2 apresentaram perfis diferentes, devido a formação de produtos intermediários de oxidação dos materiais na atmosfera de ar. A quantidade de energia liberada pela DSC e as perdas de massa observadas pelas curvas TG indicaram saturação do óleo de baru na quantidade de 24,00% (m/m. Os gases liberados analisados por TG-DSC acoplado ao FTIR, foram: água, CO2, CO e etanol. Com o aumento do óleo de baru, ocorreu maior absorção de água pelo PU, na proporção de 29,00% (m/m o índice de intumescimento foi de 91,10%, em 1440 minutos. Assim, pudemos observer a formação do poliuretano com características distintas devido a presença do OB.

  8. Preparação e caracterização da hexaferrita de bário dopada com niquel e cobalto

    Directory of Open Access Journals (Sweden)

    R. R. Corrêa

    2013-12-01

    Full Text Available O objetivo deste trabalho foi a obtenção de ferritas de bário tipo W com estequiometria BaCo2-xNi xFe16O27 por meio do método cerâmico tradicional, que é muito difundindo nas indústrias de processamento cerâmico por ser econômico e de relativa facilidade na obtenção de pós-cerâmicos. Materiais aditivados podem afetar as propriedades elétricas, magnéticas e a microestrutura das ferritas por diferentes mecanismos. Assim, temos como objetivo investigar o efeito da substituição dos elementos cobalto e níquel na microestrutura da hexaferrita tipo W com fórmula estequiométrica BaCo2-xNi xFe16O27, x = 0,0, 0,6, 1,0 e 2.

  9. 土乡荨麻情%My Passion with Nettle in Tu Villages

    Institute of Scientific and Technical Information of China (English)

    董得红

    2008-01-01

    第一次认识荨麻草是1985年的初夏在互助北山林场,那时我刚从林业学校毕业分配到省林业调查队,参加北山林场的森林调查。我们那个工组就驻扎在浪士当沟脑德前台的河滩里,这条沟里居住着汉族、土族和藏族,是多民族聚居地区。那时林区尚未开展森林旅游,除林场职工和当地农牧民,其他人很少进去,民俗原始、自然而淳朴,森林也保持着原始风貌。

  10. Querer a los animales sin descuidar tu salud (Love Animals AND Stay Safe)

    Centers for Disease Control (CDC) Podcasts

    2011-08-16

    En este podcast para niños, los chicos de Kidtastics hablan sobre la rabia y cómo prevenirla.  Created: 8/16/2011 by National Center for Emerging and Zoonotic Infectious Diseases (NCEZID).   Date Released: 1/3/2012.

  11. TU-A-17A-01: Memorial to Benjamin M. Galkin - Memorial Lecture

    Energy Technology Data Exchange (ETDEWEB)

    Suntharalingam, N [Voorhees, NJ (United States)

    2014-06-15

    This past year Medical Physics lost one of its active members, Benjamin M. Galkin. Ben Galkin was a Past-Treasurer of the AAPM. During his leadership role he played an important part in Securing membership, for the AAPM, in the American Institute of physics. As Treasurer he was also a prime mover in starting the journal, Medical Physics, and served as its business manager in the formative years.Ben Galkin received his Masters Degree at Columbia University in New York, under the mentorship of Dr. Edith Quimby, one of the pioneer Hospital Radiation Physicists in the country. He started his professional career at Jefferson Hospital in Philadelphia, joining Robert Gorson, and remained there until retirement. He served as the institution’s Radiation Safety Officer throughout his career. His research interest was Breast Imaging. He held joint faculty appointments in the Department of Radiology and the Department of Radiation Therapy and Nuclear Medicine, rising up to the rank of Full professor. He was a well respected teacher for the residents in Radiology.

  12. Elevator pitch : ¡comunica y defiende tu proyecto en 60 segundos!

    OpenAIRE

    Tena Fernández, Ramón

    2016-01-01

    La escuela del siglo XXI se esfuerza duramente en trabajar aptitudes como la cohesión grupal, el emprendimiento y la cultura del esfuerzo, con ello pretende capacitar a su alumnado para un futuro laboral, tan real como próximo. No obstante, nos olvidamos que tan importante es dominar una materia de trabajo, como el saber conseguir una oportunidad con la que poder demostrar nuestras habilidades. Eso es Elevator Pitch, una novedosa metodología didáctica, con la que aprender a presentar nuestros...

  13. Pakend - kaunis ja tüütu lisand / Villem Loigom

    Index Scriptorium Estoniae

    Loigom, Villem

    2007-01-01

    Piimapaki, suitsu- ja parfüümipakendite ajaloost ja tänapäevast. Paberist pudeli looja oli John Van Wormer, Marlboro tänapäevase sigaretipaki disainer oli Francesco Gianninoto ja üheks nimekamaks parfüümipudelite kujundajaks Fabien Baron

  14. "Ishica, de quien sos tu?": Nostalgia for a Mother Tongue in Rosa Nissan's Novels

    Science.gov (United States)

    Linhard, Tabea Alex

    2009-01-01

    This paper explores the use of Judeo-Spanish in the Rosa Nissan's novels "Novia que te vea" (1992) and "Hisho que te nazca" (1996) and reveals the ways in which the presence of this language interrupts the otherwise linear coming-of-age narrative. An analysis of the main character's relationship with Judeo-Spanish establishes a critical dialogue…

  15. Contribution of Arg288 of Escherichia coli elongation factor Tu to translational functionality

    DEFF Research Database (Denmark)

    Rattenborg, Thomas; Nautrup-Pedersen, Gitte; Clark, Brian F. C.

    1997-01-01

    -type factor with regard to the association and dissociation of guanine nucleotides, and the intrinsic GTPase activities are unchanged. Furthermore, the mutants were as efficient as the wild-type factor in carrying out protein synthesis in vitro in the presence of an excess of aminoacyl-tRNA. However...

  16. Eficacia publicitaria: El caso de IKEA y 'La República Independiente de Tu Casa'

    OpenAIRE

    Núñez Ramírez, Marta

    2014-01-01

    L'estudi pretén aprofundir en l'àmbit de l'avaluació de l'eficàcia publicitària, a través de l'anàlisi d'un cas concret: l'anunci d'IKEA 'Benvingut a la República Independent de casa teva' de l'any 2006. Primerament, s'intenta explicar en què consisteix el concepte d'eficàcia, es reflexiona sobre la seva relació o no amb les vendes que aconsegueix generar una marca, i s'obren vies sobre criteris d'avaluació útils per a la investigació. S'arriba a una primera observació: la importància dels ob...

  17. TU-G-BRD-04: A Round Robin Dosimetry Intercomparison of Gamma Stereotactic Radiosurgery Calibration Protocols

    Energy Technology Data Exchange (ETDEWEB)

    Drzymala, R [Washington University, Saint Louis, MO (United States); Alvarez, P [University of Texas MD Anderson Cancer Center, Houston, TX (United States); Bednarz, G [University of Pittsburgh Medical Center, Pittsburgh, PA (United States); Bourland, J [Wake Forest University, Winston-salem, NC (United States); DeWerd, L [University of Wisconsin, Madison/ADCL, Madison, WI (United States); Ma, L [University of California San Francisco Comprehensive Cancer Center, San Francisco, CA (United States); Meltsner, S [Duke University Medical Center, Durham, NC (United States); Neyman, G [The Cleveland Clinic Foundation, Beachwood, OH (United States); Novotny, J [Hospital Na Homolce, Prague (Czech Republic); Petti, P [Washington Hospital, Fremont, CA (United States); Rivard, M [Tufts University School of Medicine, Boston, MA (United States); Shiu, A [University of Southern California, Los Angeles, CA (United States); Goetsch, S [Dade Moeller Health Group, La Jolla, CA (United States)

    2015-06-15

    Purpose: The purpose of this multi-institutional study was to compare two new gamma stereotactic radiosurgery (GSRS) dosimetry protocols to existing calibration methods. The ultimate goal was to guide AAPM Task Group 178 in recommending a standard GSRS dosimetry protocol. Methods: Nine centers (ten GSRS units) participated in the study. Each institution made eight sets of dose rate measurements: six with two different ionization chambers in three different 160mm-diameter spherical phantoms (ABS plastic, Solid Water and liquid water), and two using the same ionization chambers with a custom in-air positioning jig. Absolute dose rates were calculated using a newly proposed formalism by the IAEA working group for small and non-standard radiation fields and with a new air-kerma based protocol. The new IAEA protocol requires an in-water ionization chamber calibration and uses previously reported Monte-Carlo generated factors to account for the material composition of the phantom, the type of ionization chamber, and the unique GSRS beam configuration. Results obtained with the new dose calibration protocols were compared to dose rates determined by the AAPM TG-21 and TG-51 protocols, with TG-21 considered as the standard. Results: Averaged over all institutions, ionization chambers and phantoms, the mean dose rate determined with the new IAEA protocol relative to that determined with TG-21 in the ABS phantom was 1.000 with a standard deviation of 0.008. For TG-51, the average ratio was 0.991 with a standard deviation of 0.013, and for the new in-air formalism it was 1.008 with a standard deviation of 0.012. Conclusion: Average results with both of the new protocols agreed with TG-21 to within one standard deviation. TG-51, which does not take into account the unique GSRS beam configuration or phantom material, was not expected to perform as well as the new protocols. The new IAEA protocol showed remarkably good agreement with TG-21. Conflict of Interests: Paula Petti, Josef Novotny, Gennady Neyman and Steve Goetsch are consultants for Elekta Instrument A/B; Elekta Instrument AB, PTW Freiburg GmbH, Standard Imaging, Inc., and The Phantom Laboratory, Inc. loaned equipment for use in these experiments; The University of Wisconsin Accredited Dosimetry Calibration Laboratory provided calibration services.

  18. TU-F-BRF-06: 3D Pancreas MRI Segmentation Using Dictionary Learning and Manifold Clustering

    Energy Technology Data Exchange (ETDEWEB)

    Gou, S; Rapacchi, S; Hu, P; Sheng, K [UCLA School of Medicine, Los Angeles, CA (United States)

    2014-06-15

    Purpose: The recent advent of MRI guided radiotherapy machines has lent an exciting platform for soft tissue target localization during treatment. However, tools to efficiently utilize MRI images for such purpose have not been developed. Specifically, to efficiently quantify the organ motion, we develop an automated segmentation method using dictionary learning and manifold clustering (DLMC). Methods: Fast 3D HASTE and VIBE MR images of 2 healthy volunteers and 3 patients were acquired. A bounding box was defined to include pancreas and surrounding normal organs including the liver, duodenum and stomach. The first slice of the MRI was used for dictionary learning based on mean-shift clustering and K-SVD sparse representation. Subsequent images were iteratively reconstructed until the error is less than a preset threshold. The preliminarily segmentation was subject to the constraints of manifold clustering. The segmentation results were compared with the mean shift merging (MSM), level set (LS) and manual segmentation methods. Results: DLMC resulted in consistently higher accuracy and robustness than comparing methods. Using manual contours as the ground truth, the mean Dices indices for all subjects are 0.54, 0.56 and 0.67 for MSM, LS and DLMC, respectively based on the HASTE image. The mean Dices indices are 0.70, 0.77 and 0.79 for the three methods based on VIBE images. DLMC is clearly more robust on the patients with the diseased pancreas while LS and MSM tend to over-segment the pancreas. DLMC also achieved higher sensitivity (0.80) and specificity (0.99) combining both imaging techniques. LS achieved equivalent sensitivity on VIBE images but was more computationally inefficient. Conclusion: We showed that pancreas and surrounding normal organs can be reliably segmented based on fast MRI using DLMC. This method will facilitate both planning volume definition and imaging guidance during treatment.

  19. TU-C-9A-01: IROC Organization and Clinical Trial Credentialing

    Energy Technology Data Exchange (ETDEWEB)

    Followill, D; Molineu, A [UT MD Anderson Cancer Center, Houston, TX (United States); Xiao, Y [Thomas Jefferson University Hospital, Philadelphia, PA (United States)

    2014-06-15

    As a response to recommendations from a report from the Institute of Medicine, NCI is reorganizing it clinical trial groups into a National Clinical Trial Network (NCTN) that consists of four adult groups (Alliance, ECOGACRIN, NRG, and SWOG) and one children’s group (COG). NRG will house CIRO, a center to promote innovative radiation therapy research and intergroup collaboration in radiation. The quality assurance groups that support clinical trials have also been restructured. ITC, OSU Imaging corelab, Philadelphia Imaging core-lab, QARC, RPC, and RTOGQA have joined together to create the Imaging and Radiation Oncology Core (IROC) Group. IROC’s mission is to provide integrated radiation oncology and diagnostic imaging quality control programs in support of the NCI’s NCTN thereby assuring high quality data for clinical trials designed to improve the clinical outcomes for cancer patients worldwide. This will be accomplished through five core services: site qualification, trial design support, credentialing, data management, case review.These changes are important for physicist participating in NCI clinical trials to understand. We will describe in detail the IROC’s activities and five core services so that as a user, the medical physicist can learn how to efficiently utilize this group. We will describe common pitfalls encountered in credentialing for current protocols and present methods to avoid them. These may include the which benchmarks are required for NSABP B-51/RTOG 1304 and how to plan them as well as tips for phantom planning. We will explain how to submit patient and phantom cases in the TRIAD system used by IROC. Learning Objectives: To understand the basic organization of IROC, its mission and five core services To learn how to use TRIAD for patient and phantom data submission To learn how to avoid common pitfalls in credentialing for current trials.

  20. TU-F-CAMPUS-T-04: Using Gold Nanoparticles to Target Mitochondria in Radiation Therapy

    Energy Technology Data Exchange (ETDEWEB)

    McNamara, A [Massachusetts General Hospital & Harvard Med. Sch., Boston, MA (United States); McMahon, S [Massachusetts General Hospital, Boston, Ma (United States); Lin, Y [Massachusetts General Hospital and Harvard Medical School, Boston, MA (United States); Paganetti, H; Schuemann, J [Massachusetts General Hospital, Boston, MA (United States); Kuncic, Z [University of Sydney, Sydney, NSW (Australia)

    2015-06-15

    Purpose: The mitochondrion, like the cell nucleus, contains genetic material and plays several critical roles that determine the cell viability, including neutralization of free radicals within the cell. Studies have shown that irradiated cells with impaired mitochondria will incur more damage to the cell nucleus. This study investigates the potential use of GNPs to enhance radiation-induced damage to the organelle. Methods: The compositions of the organelles of a JURKAT cell were determined experimentally. Using Monte Carlo simulations, we investigate the significance of dose enhancement in a monoenergetic (10–50 keV and 6 MeV) x-ray irradiated cell cytoplasm, consisting of the experimentally determined composition. We also investigate the track structure of secondary electrons in the mitochondria using Geant4-DNA in the presence and absence of GNPs for incident protons and photons. The biological effect was determined using an approach based on the local effect model, assuming the mitochondrial DNA (mtDNA) was the primary target. Results: Adding 0.01% of gold to the cell cytoplasm material can cause substantial dose enhancement, dependent on the incident x-ray energy. Track structure Monte Carlo (MC) simulations show an increased number of ionization events within the mitochondrion structure. The close proximity of GNPs to the mtDNA storing nucleoid may cause the mtDNA to receive doses above ∼100 Gy for keV x-rays, leading to mitochondrial dysfunction. Conclusion: A substantial increase in ionization events can occur in the mitochondria in the presence of GNPs. If GNPs can be delivered to tumors and attached to a sufficient number of mitochondria inside the tumor cells, mitochondrial induced cell death could be a prevalent cause of cell death. The biological structures developed here will be included in the biological MC toolkit, TOPAS-nBio.

  1. TU-AB-204-04: Advances in CBCT for Breast Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Boone, J. [University of California Davis School of Medicine (United States)

    2015-06-15

    This symposium highlights advanced cone-beam CT (CBCT) technologies in four areas of emerging application in diagnostic imaging and image-guided interventions. Each area includes research that extends the spatial, temporal, and/or contrast resolution characteristics of CBCT beyond conventional limits through advances in scanner technology, acquisition protocols, and 3D image reconstruction techniques. Dr. G. Chen (University of Wisconsin) will present on the topic: Advances in C-arm CBCT for Brain Perfusion Imaging. Stroke is a leading cause of death and disability, and a fraction of people having an acute ischemic stroke are suitable candidates for endovascular therapy. Critical factors that affect both the likelihood of successful revascularization and good clinical outcome are: 1) the time between stroke onset and revascularization; and 2) the ability to distinguish patients who have a small volume of irreversibly injured brain (ischemic core) and a large volume of ischemic but salvageable brain (penumbra) from patients with a large ischemic core and little or no penumbra. Therefore, “time is brain” in the care of the stroke patients. C-arm CBCT systems widely available in angiography suites have the potential to generate non-contrast-enhanced CBCT images to exclude the presence of hemorrhage, time-resolved CBCT angiography to evaluate the site of occlusion and collaterals, and CBCT perfusion parametric images to assess the extent of the ischemic core and penumbra, thereby fulfilling the imaging requirements of a “one-stop-shop” in the angiography suite to reduce the time between onset and revascularization therapy. The challenges and opportunities to advance CBCT technology to fully enable the one-stop-shop C-arm CBCT platform for brain imaging will be discussed. Dr. R. Fahrig (Stanford University) will present on the topic: Advances in C-arm CBCT for Cardiac Interventions. With the goal of providing functional information during cardiac interventions, significant effort has been expended to improve the quantitative accuracy of C-arm CBCT reconstructions. The challenge is to improve image quality while providing very short turnaround between data acquisition and volume data visualization. Corrections for x-ray scatter, view aliasing and patient motion that require no more than 2 iterations keep processing time short while reducing artifact. Fast, multi-sweep acquisitions can be used to permit assessment of left ventricular function, and visualization of radiofrequency lesions created to treat arrhythmias. Workflows for each imaging goal have been developed and validated against gold standard clinical CT or histology. The challenges, opportunities, and limitations of the new functional C-arm CBCT imaging techniques will be discussed. Dr. W. Zbijewski (Johns Hopkins University) will present on the topic: Advances in CBCT for Orthopaedics and Bone Health Imaging. Cone-beam CT is particularly well suited for imaging of musculoskeletal extremities. Owing to the high spatial resolution of flat-panel detectors, CBCT can surpass conventional CT in imaging tasks involving bone visualization, quantitative analysis of subchondral trabecular structure, and visualization and monitoring of subtle fractures that are common in orthopedic radiology. A dedicated CBCT platform has been developed that offers flexibility in system design and provides not only a compact configuration with improved logistics for extremities imaging but also enables novel diagnostic capabilities such as imaging of weight-bearing lower extremities in a natural stance. The design, development and clinical performance of dedicated extremities CBCT systems will be presented. Advanced capabilities for quantitative volumetric assessment of joint space morphology, dual-energy image-based quantification of bone composition, and in-vivo analysis of bone microarchitecture will be discussed, along with emerging applications in the diagnosis of arthritis and osteoporosis and assessment of novel therapies. Finally, Dr. J. Boone (UC Davis) will present on the topic: Advances in CBCT for Breast Imaging. Breast CT has been studied as an imaging tool for diagnostic breast evaluation and for potential breast cancer screening. The breast CT application lends itself to CBCT because of the small dimensions of the breast, the tapered shape of the breast towards higher cone angle, and the fact that there are no bones in the breast. The performance of various generations of breast CT scanners developed in recent years will be discussed, focusing on advances in spatial resolution and image noise characteristics. The results will also demonstrate the results of clinical trials using both computer and human observers. Learning Objectives: Understand the challenges, key technological advances, and emerging opportunities of CBCT in: Brain perfusion imaging, including assessment of ischemic stroke Cardiac imaging for functional assessment in cardiac interventions Orthopedics imaging for evaluation of musculoskeletal trauma, arthritis, and osteoporosis Breast imaging for screening and diagnosis of breast cancer. Work presented in this symposium includes research support by: Siemens Healthcare (Dr. Chen); NIH and Siemens Healthcare (Dr. Fahrig); NIH and Carestream Health (Dr. Zbijewski); and NIH (Dr. Boone)

  2. TU-AB-204-03: Advances in CBCT for Orhtopaedics and Bone Health Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Zbijewski, W. [Johns Hopkins University (United States)

    2015-06-15

    This symposium highlights advanced cone-beam CT (CBCT) technologies in four areas of emerging application in diagnostic imaging and image-guided interventions. Each area includes research that extends the spatial, temporal, and/or contrast resolution characteristics of CBCT beyond conventional limits through advances in scanner technology, acquisition protocols, and 3D image reconstruction techniques. Dr. G. Chen (University of Wisconsin) will present on the topic: Advances in C-arm CBCT for Brain Perfusion Imaging. Stroke is a leading cause of death and disability, and a fraction of people having an acute ischemic stroke are suitable candidates for endovascular therapy. Critical factors that affect both the likelihood of successful revascularization and good clinical outcome are: 1) the time between stroke onset and revascularization; and 2) the ability to distinguish patients who have a small volume of irreversibly injured brain (ischemic core) and a large volume of ischemic but salvageable brain (penumbra) from patients with a large ischemic core and little or no penumbra. Therefore, “time is brain” in the care of the stroke patients. C-arm CBCT systems widely available in angiography suites have the potential to generate non-contrast-enhanced CBCT images to exclude the presence of hemorrhage, time-resolved CBCT angiography to evaluate the site of occlusion and collaterals, and CBCT perfusion parametric images to assess the extent of the ischemic core and penumbra, thereby fulfilling the imaging requirements of a “one-stop-shop” in the angiography suite to reduce the time between onset and revascularization therapy. The challenges and opportunities to advance CBCT technology to fully enable the one-stop-shop C-arm CBCT platform for brain imaging will be discussed. Dr. R. Fahrig (Stanford University) will present on the topic: Advances in C-arm CBCT for Cardiac Interventions. With the goal of providing functional information during cardiac interventions, significant effort has been expended to improve the quantitative accuracy of C-arm CBCT reconstructions. The challenge is to improve image quality while providing very short turnaround between data acquisition and volume data visualization. Corrections for x-ray scatter, view aliasing and patient motion that require no more than 2 iterations keep processing time short while reducing artifact. Fast, multi-sweep acquisitions can be used to permit assessment of left ventricular function, and visualization of radiofrequency lesions created to treat arrhythmias. Workflows for each imaging goal have been developed and validated against gold standard clinical CT or histology. The challenges, opportunities, and limitations of the new functional C-arm CBCT imaging techniques will be discussed. Dr. W. Zbijewski (Johns Hopkins University) will present on the topic: Advances in CBCT for Orthopaedics and Bone Health Imaging. Cone-beam CT is particularly well suited for imaging of musculoskeletal extremities. Owing to the high spatial resolution of flat-panel detectors, CBCT can surpass conventional CT in imaging tasks involving bone visualization, quantitative analysis of subchondral trabecular structure, and visualization and monitoring of subtle fractures that are common in orthopedic radiology. A dedicated CBCT platform has been developed that offers flexibility in system design and provides not only a compact configuration with improved logistics for extremities imaging but also enables novel diagnostic capabilities such as imaging of weight-bearing lower extremities in a natural stance. The design, development and clinical performance of dedicated extremities CBCT systems will be presented. Advanced capabilities for quantitative volumetric assessment of joint space morphology, dual-energy image-based quantification of bone composition, and in-vivo analysis of bone microarchitecture will be discussed, along with emerging applications in the diagnosis of arthritis and osteoporosis and assessment of novel therapies. Finally, Dr. J. Boone (UC Davis) will present on the topic: Advances in CBCT for Breast Imaging. Breast CT has been studied as an imaging tool for diagnostic breast evaluation and for potential breast cancer screening. The breast CT application lends itself to CBCT because of the small dimensions of the breast, the tapered shape of the breast towards higher cone angle, and the fact that there are no bones in the breast. The performance of various generations of breast CT scanners developed in recent years will be discussed, focusing on advances in spatial resolution and image noise characteristics. The results will also demonstrate the results of clinical trials using both computer and human observers. Learning Objectives: Understand the challenges, key technological advances, and emerging opportunities of CBCT in: Brain perfusion imaging, including assessment of ischemic stroke Cardiac imaging for functional assessment in cardiac interventions Orthopedics imaging for evaluation of musculoskeletal trauma, arthritis, and osteoporosis Breast imaging for screening and diagnosis of breast cancer. Work presented in this symposium includes research support by: Siemens Healthcare (Dr. Chen); NIH and Siemens Healthcare (Dr. Fahrig); NIH and Carestream Health (Dr. Zbijewski); and NIH (Dr. Boone)

  3. TU-AB-204-02: Advances in C-Arm CBCT for Cardiac Interventions

    Energy Technology Data Exchange (ETDEWEB)

    Fahrig, R. [Stanford University (United States)

    2015-06-15

    This symposium highlights advanced cone-beam CT (CBCT) technologies in four areas of emerging application in diagnostic imaging and image-guided interventions. Each area includes research that extends the spatial, temporal, and/or contrast resolution characteristics of CBCT beyond conventional limits through advances in scanner technology, acquisition protocols, and 3D image reconstruction techniques. Dr. G. Chen (University of Wisconsin) will present on the topic: Advances in C-arm CBCT for Brain Perfusion Imaging. Stroke is a leading cause of death and disability, and a fraction of people having an acute ischemic stroke are suitable candidates for endovascular therapy. Critical factors that affect both the likelihood of successful revascularization and good clinical outcome are: 1) the time between stroke onset and revascularization; and 2) the ability to distinguish patients who have a small volume of irreversibly injured brain (ischemic core) and a large volume of ischemic but salvageable brain (penumbra) from patients with a large ischemic core and little or no penumbra. Therefore, “time is brain” in the care of the stroke patients. C-arm CBCT systems widely available in angiography suites have the potential to generate non-contrast-enhanced CBCT images to exclude the presence of hemorrhage, time-resolved CBCT angiography to evaluate the site of occlusion and collaterals, and CBCT perfusion parametric images to assess the extent of the ischemic core and penumbra, thereby fulfilling the imaging requirements of a “one-stop-shop” in the angiography suite to reduce the time between onset and revascularization therapy. The challenges and opportunities to advance CBCT technology to fully enable the one-stop-shop C-arm CBCT platform for brain imaging will be discussed. Dr. R. Fahrig (Stanford University) will present on the topic: Advances in C-arm CBCT for Cardiac Interventions. With the goal of providing functional information during cardiac interventions, significant effort has been expended to improve the quantitative accuracy of C-arm CBCT reconstructions. The challenge is to improve image quality while providing very short turnaround between data acquisition and volume data visualization. Corrections for x-ray scatter, view aliasing and patient motion that require no more than 2 iterations keep processing time short while reducing artifact. Fast, multi-sweep acquisitions can be used to permit assessment of left ventricular function, and visualization of radiofrequency lesions created to treat arrhythmias. Workflows for each imaging goal have been developed and validated against gold standard clinical CT or histology. The challenges, opportunities, and limitations of the new functional C-arm CBCT imaging techniques will be discussed. Dr. W. Zbijewski (Johns Hopkins University) will present on the topic: Advances in CBCT for Orthopaedics and Bone Health Imaging. Cone-beam CT is particularly well suited for imaging of musculoskeletal extremities. Owing to the high spatial resolution of flat-panel detectors, CBCT can surpass conventional CT in imaging tasks involving bone visualization, quantitative analysis of subchondral trabecular structure, and visualization and monitoring of subtle fractures that are common in orthopedic radiology. A dedicated CBCT platform has been developed that offers flexibility in system design and provides not only a compact configuration with improved logistics for extremities imaging but also enables novel diagnostic capabilities such as imaging of weight-bearing lower extremities in a natural stance. The design, development and clinical performance of dedicated extremities CBCT systems will be presented. Advanced capabilities for quantitative volumetric assessment of joint space morphology, dual-energy image-based quantification of bone composition, and in-vivo analysis of bone microarchitecture will be discussed, along with emerging applications in the diagnosis of arthritis and osteoporosis and assessment of novel therapies. Finally, Dr. J. Boone (UC Davis) will present on the topic: Advances in CBCT for Breast Imaging. Breast CT has been studied as an imaging tool for diagnostic breast evaluation and for potential breast cancer screening. The breast CT application lends itself to CBCT because of the small dimensions of the breast, the tapered shape of the breast towards higher cone angle, and the fact that there are no bones in the breast. The performance of various generations of breast CT scanners developed in recent years will be discussed, focusing on advances in spatial resolution and image noise characteristics. The results will also demonstrate the results of clinical trials using both computer and human observers. Learning Objectives: Understand the challenges, key technological advances, and emerging opportunities of CBCT in: Brain perfusion imaging, including assessment of ischemic stroke Cardiac imaging for functional assessment in cardiac interventions Orthopedics imaging for evaluation of musculoskeletal trauma, arthritis, and osteoporosis Breast imaging for screening and diagnosis of breast cancer. Work presented in this symposium includes research support by: Siemens Healthcare (Dr. Chen); NIH and Siemens Healthcare (Dr. Fahrig); NIH and Carestream Health (Dr. Zbijewski); and NIH (Dr. Boone)

  4. TU-AB-204-00: Advances in Cone-Beam CT and Emerging Applications

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2015-06-15

    This symposium highlights advanced cone-beam CT (CBCT) technologies in four areas of emerging application in diagnostic imaging and image-guided interventions. Each area includes research that extends the spatial, temporal, and/or contrast resolution characteristics of CBCT beyond conventional limits through advances in scanner technology, acquisition protocols, and 3D image reconstruction techniques. Dr. G. Chen (University of Wisconsin) will present on the topic: Advances in C-arm CBCT for Brain Perfusion Imaging. Stroke is a leading cause of death and disability, and a fraction of people having an acute ischemic stroke are suitable candidates for endovascular therapy. Critical factors that affect both the likelihood of successful revascularization and good clinical outcome are: 1) the time between stroke onset and revascularization; and 2) the ability to distinguish patients who have a small volume of irreversibly injured brain (ischemic core) and a large volume of ischemic but salvageable brain (penumbra) from patients with a large ischemic core and little or no penumbra. Therefore, “time is brain” in the care of the stroke patients. C-arm CBCT systems widely available in angiography suites have the potential to generate non-contrast-enhanced CBCT images to exclude the presence of hemorrhage, time-resolved CBCT angiography to evaluate the site of occlusion and collaterals, and CBCT perfusion parametric images to assess the extent of the ischemic core and penumbra, thereby fulfilling the imaging requirements of a “one-stop-shop” in the angiography suite to reduce the time between onset and revascularization therapy. The challenges and opportunities to advance CBCT technology to fully enable the one-stop-shop C-arm CBCT platform for brain imaging will be discussed. Dr. R. Fahrig (Stanford University) will present on the topic: Advances in C-arm CBCT for Cardiac Interventions. With the goal of providing functional information during cardiac interventions, significant effort has been expended to improve the quantitative accuracy of C-arm CBCT reconstructions. The challenge is to improve image quality while providing very short turnaround between data acquisition and volume data visualization. Corrections for x-ray scatter, view aliasing and patient motion that require no more than 2 iterations keep processing time short while reducing artifact. Fast, multi-sweep acquisitions can be used to permit assessment of left ventricular function, and visualization of radiofrequency lesions created to treat arrhythmias. Workflows for each imaging goal have been developed and validated against gold standard clinical CT or histology. The challenges, opportunities, and limitations of the new functional C-arm CBCT imaging techniques will be discussed. Dr. W. Zbijewski (Johns Hopkins University) will present on the topic: Advances in CBCT for Orthopaedics and Bone Health Imaging. Cone-beam CT is particularly well suited for imaging of musculoskeletal extremities. Owing to the high spatial resolution of flat-panel detectors, CBCT can surpass conventional CT in imaging tasks involving bone visualization, quantitative analysis of subchondral trabecular structure, and visualization and monitoring of subtle fractures that are common in orthopedic radiology. A dedicated CBCT platform has been developed that offers flexibility in system design and provides not only a compact configuration with improved logistics for extremities imaging but also enables novel diagnostic capabilities such as imaging of weight-bearing lower extremities in a natural stance. The design, development and clinical performance of dedicated extremities CBCT systems will be presented. Advanced capabilities for quantitative volumetric assessment of joint space morphology, dual-energy image-based quantification of bone composition, and in-vivo analysis of bone microarchitecture will be discussed, along with emerging applications in the diagnosis of arthritis and osteoporosis and assessment of novel therapies. Finally, Dr. J. Boone (UC Davis) will present on the topic: Advances in CBCT for Breast Imaging. Breast CT has been studied as an imaging tool for diagnostic breast evaluation and for potential breast cancer screening. The breast CT application lends itself to CBCT because of the small dimensions of the breast, the tapered shape of the breast towards higher cone angle, and the fact that there are no bones in the breast. The performance of various generations of breast CT scanners developed in recent years will be discussed, focusing on advances in spatial resolution and image noise characteristics. The results will also demonstrate the results of clinical trials using both computer and human observers. Learning Objectives: Understand the challenges, key technological advances, and emerging opportunities of CBCT in: Brain perfusion imaging, including assessment of ischemic stroke Cardiac imaging for functional assessment in cardiac interventions Orthopedics imaging for evaluation of musculoskeletal trauma, arthritis, and osteoporosis Breast imaging for screening and diagnosis of breast cancer. Work presented in this symposium includes research support by: Siemens Healthcare (Dr. Chen); NIH and Siemens Healthcare (Dr. Fahrig); NIH and Carestream Health (Dr. Zbijewski); and NIH (Dr. Boone)

  5. TU-AB-204-01: Advances in C-Arm CBCT for Brain Perfusion Imaging

    Energy Technology Data Exchange (ETDEWEB)

    Chen, G. [University of Wisconsin (United States)

    2015-06-15

    This symposium highlights advanced cone-beam CT (CBCT) technologies in four areas of emerging application in diagnostic imaging and image-guided interventions. Each area includes research that extends the spatial, temporal, and/or contrast resolution characteristics of CBCT beyond conventional limits through advances in scanner technology, acquisition protocols, and 3D image reconstruction techniques. Dr. G. Chen (University of Wisconsin) will present on the topic: Advances in C-arm CBCT for Brain Perfusion Imaging. Stroke is a leading cause of death and disability, and a fraction of people having an acute ischemic stroke are suitable candidates for endovascular therapy. Critical factors that affect both the likelihood of successful revascularization and good clinical outcome are: 1) the time between stroke onset and revascularization; and 2) the ability to distinguish patients who have a small volume of irreversibly injured brain (ischemic core) and a large volume of ischemic but salvageable brain (penumbra) from patients with a large ischemic core and little or no penumbra. Therefore, “time is brain” in the care of the stroke patients. C-arm CBCT systems widely available in angiography suites have the potential to generate non-contrast-enhanced CBCT images to exclude the presence of hemorrhage, time-resolved CBCT angiography to evaluate the site of occlusion and collaterals, and CBCT perfusion parametric images to assess the extent of the ischemic core and penumbra, thereby fulfilling the imaging requirements of a “one-stop-shop” in the angiography suite to reduce the time between onset and revascularization therapy. The challenges and opportunities to advance CBCT technology to fully enable the one-stop-shop C-arm CBCT platform for brain imaging will be discussed. Dr. R. Fahrig (Stanford University) will present on the topic: Advances in C-arm CBCT for Cardiac Interventions. With the goal of providing functional information during cardiac interventions, significant effort has been expended to improve the quantitative accuracy of C-arm CBCT reconstructions. The challenge is to improve image quality while providing very short turnaround between data acquisition and volume data visualization. Corrections for x-ray scatter, view aliasing and patient motion that require no more than 2 iterations keep processing time short while reducing artifact. Fast, multi-sweep acquisitions can be used to permit assessment of left ventricular function, and visualization of radiofrequency lesions created to treat arrhythmias. Workflows for each imaging goal have been developed and validated against gold standard clinical CT or histology. The challenges, opportunities, and limitations of the new functional C-arm CBCT imaging techniques will be discussed. Dr. W. Zbijewski (Johns Hopkins University) will present on the topic: Advances in CBCT for Orthopaedics and Bone Health Imaging. Cone-beam CT is particularly well suited for imaging of musculoskeletal extremities. Owing to the high spatial resolution of flat-panel detectors, CBCT can surpass conventional CT in imaging tasks involving bone visualization, quantitative analysis of subchondral trabecular structure, and visualization and monitoring of subtle fractures that are common in orthopedic radiology. A dedicated CBCT platform has been developed that offers flexibility in system design and provides not only a compact configuration with improved logistics for extremities imaging but also enables novel diagnostic capabilities such as imaging of weight-bearing lower extremities in a natural stance. The design, development and clinical performance of dedicated extremities CBCT systems will be presented. Advanced capabilities for quantitative volumetric assessment of joint space morphology, dual-energy image-based quantification of bone composition, and in-vivo analysis of bone microarchitecture will be discussed, along with emerging applications in the diagnosis of arthritis and osteoporosis and assessment of novel therapies. Finally, Dr. J. Boone (UC Davis) will present on the topic: Advances in CBCT for Breast Imaging. Breast CT has been studied as an imaging tool for diagnostic breast evaluation and for potential breast cancer screening. The breast CT application lends itself to CBCT because of the small dimensions of the breast, the tapered shape of the breast towards higher cone angle, and the fact that there are no bones in the breast. The performance of various generations of breast CT scanners developed in recent years will be discussed, focusing on advances in spatial resolution and image noise characteristics. The results will also demonstrate the results of clinical trials using both computer and human observers. Learning Objectives: Understand the challenges, key technological advances, and emerging opportunities of CBCT in: Brain perfusion imaging, including assessment of ischemic stroke Cardiac imaging for functional assessment in cardiac interventions Orthopedics imaging for evaluation of musculoskeletal trauma, arthritis, and osteoporosis Breast imaging for screening and diagnosis of breast cancer. Work presented in this symposium includes research support by: Siemens Healthcare (Dr. Chen); NIH and Siemens Healthcare (Dr. Fahrig); NIH and Carestream Health (Dr. Zbijewski); and NIH (Dr. Boone)

  6. Marketing online para investigadores : diez formas de divulgar tu trabajo en la red

    OpenAIRE

    Domínguez Fernández, Alejandro

    2012-01-01

    En este artículo recogemos diez técnicas habituales del marketing online que suelen aplicar las empresas para promocionar y aumentar la visisbilidad de sus productos y servicios en la red y que son perfectamente válidas para dar a conocer los resultados de investigación y conseguir así repercusión en la sociedad

  7. A multiplicative potential approach to solutions for cooperative TU-games

    NARCIS (Netherlands)

    Driessen, T.S.H.; Calvo, E.

    2001-01-01

    Concerning the solution theory for cooperative games with transferable utility, it is well-known that the Shapley value is the most appealing representative of the family of (not necessarily efficient) game-theoretic solutions with an additive potential representation. This paper introduces a new so

  8. TU-F-CAMPUS-T-03: Enhancing the Tumor Specific Radiosensitization Using Molecular Targeted Gold Nanorods

    Energy Technology Data Exchange (ETDEWEB)

    Diagaradjane, P [M.D. Anderson Cancer Center, Houston, TX (United States); Deorukhkar, A; Sankaranarayanapillai, M; Singh, P [The UT MD Anderson Cancer Center, Houston, TX (United States); Manohar, N; Tailor, R; Cho, S [UT MD Anderson Cancer Center, Houston, TX (United States); Goodrich, G [Nanospectra Biosciences Inc, Houston, TX (United States); Krishnan, S [The University of Texas MD Anderson Cancer Center, Houston, TX (United States)

    2015-06-15

    Purpose: Gold nanoparticle (GNP) mediated radiosensitization has gained significant attention in recent years. However, the widely used passive targeting strategy requires high concentration of GNPs to induce the desired therapeutic effect, thus dampening the enthusiasm for clinical translation. The purpose of this study is to utilize a molecular targeting strategy to minimize the concentration of GNPs injected while simultaneously enhancing the tumor specific radiosensitization for an improved therapeutic outcome. Methods: Cetuximab (antibody specific to the epidermal growth factor receptor that is over-expressed in tumors) conjugated gold nanorods (cGNRs) was used for the tumor targeting. The binding affinity, internalization, and in vitro radiosensitization were evaluated using dark field microscopy, transmission electron microscopy, and clonogenic cell survival assay, respectively. In vivo biodistribution in tumor (HCT116-colorectal cancer cells) bearing mice were quantified using inductively coupled plasma mass spectrometry. In vivo radiosensitization potential was tested using 250-kVp x-rays and clinically relevant 6-MV radiation beams. Results: cGNRs displayed excellent cell-surface binding and internalization (∼31,000 vs 12,000/cell) when compared to unconjugated GNRs (pGNRs). In vitro, the dose enhancement factor at 10% survival (DEF10) was estimated as 1.06 and 1.17, respectively for both 250-kVp and 6-MV beams. In vivo biodistribution analysis revealed enhanced uptake of cGNRs in tumor (1.3 µg/g of tumor tissue), which is ∼1000-fold less than the reported values using passive targeting strategy. Nonetheless, significant radiosensitization was observed in vivo with cGNRs when compared to pGNRs, when irradiated with 250-kVp (tumor volume doubling time 35 days vs 25 days; p=0.002) and 6 MV (17 days vs 13 days; p=0.0052) beams. Conclusion: The enhanced radiosensitization effect observed with very low intratumoral concentrations of gold and megavoltage x-rays using the active targeting strategy holds promise for clinical translation of this strategy from a toxicity and cost-effectiveness perspective and could evolve as a paradigm-changing approach in the field of radiation oncology.

  9. "Tu Necesitas Preguntar en Espanol": Negotiating Good Language Learner Identity in a Spanish Classroom

    Science.gov (United States)

    Pomerantz, Anne

    2008-01-01

    This article builds on Norton and Toohey's (2001) critique of good language learner (GLL) research to illustrate how college students in an advanced Spanish conversation course drew on particular ideologies of language and foreign language learning to construct and negotiate their classroom identities. I argue that these ideologies were implicated…

  10. TU-G-BRD-02: Automated Systematic Quality Assurance Program for Radiation Oncology Information System Upgrades

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, B; Yi, B; Eley, J; Mutaf, Y; Rahman, S; D’Souza, W [University of Maryland School of Medicine, Baltimore, MD (United States)

    2015-06-15

    Purpose: To: (1) describe an independent, automated, systematic software-based protocol for verifying clinical data accuracy/integrity for mitigation of data corruption/loss risks following radiation oncology information system (ROIS) upgrades; and (2) report on application of this approach in an academic/community practice environment. Methods: We propose a robust approach to perform quality assurance on the ROIS after an upgrade, targeting four data sources: (1) ROIS relational database; (2) ROIS DICOM interface; (3) ROIS treatment machine data configuration; and (4) ROIS-generated clinical reports. We investigated the database schema for differences between pre-/post-upgrade states. Paired DICOM data streams for the same object (such as RT-Plan/Treatment Record) were compared between pre-/post-upgrade states for data corruption. We examined machine configuration and related commissioning data files for changes and corruption. ROIS-generated treatment appointment and treatment parameter reports were compared to ensure patient encounter and treatment plan accuracy. This protocol was supplemented by an end-to-end clinical workflow test to verify essential ROI functionality and integrity of components interfaced during patient care chain of activities. We describe the implementation of this protocol during a Varian ARIA system upgrade at our clinic. Results: We verified 1,638 data tables with 2.4 billion data records. For 222 under-treatment patients, 605 DICOM RT plans and 13,480 DICOM treatment records retrieved from the ROIS DICOM interface were compared, with no differences in fractions, doses delivered, or treatment parameters. We identified 82 new data tables and 78 amended/deleted tables consistent with the upgrade. Reports for 5,073 patient encounters over a 2-week horizon were compared and were identical to those before the upgrade. Content in 12,237 xml machine files was compared, with no differences identified. Conclusion: An independent QA/validation approach for ROIS upgrades was developed and implemented at our clinic. The success of this approach ensures a robust QA of ROIS upgrades without manual paper/electronic checks and associated intensive labor.

  11. TU-A-304-01: Introduction and Workflow of Image-Guided SBRT

    Energy Technology Data Exchange (ETDEWEB)

    Salter, B. [University of Utah Huntsman Cancer Institute (United States)

    2015-06-15

    Increased use of SBRT and hypo fractionation in radiation oncology practice has posted a number of challenges to medical physicist, ranging from planning, image-guided patient setup and on-treatment monitoring, to quality assurance (QA) and dose delivery. This symposium is designed to provide updated knowledge necessary for the safe and efficient implementation of SBRT in various linac platforms, including the emerging digital linacs equipped with high dose rate FFF beams. Issues related to 4D CT, PET and MRI simulations, 3D/4D CBCT guided patient setup, real-time image guidance during SBRT dose delivery using gated/un-gated VMAT or IMRT, and technical advancements in QA of SBRT (in particular, strategies dealing with high dose rate FFF beams) will be addressed. The symposium will help the attendees to gain a comprehensive understanding of the SBRT workflow and facilitate their clinical implementation of the state-of-art imaging and planning techniques. Learning Objectives: Present background knowledge of SBRT, describe essential requirements for safe implementation of SBRT, and discuss issues specific to SBRT treatment planning and QA. Update on the use of multi-dimensional (3D and 4D) and multi-modality (CT, beam-level X-ray imaging, pre- and on-treatment 3D/4D MRI, PET, robotic ultrasound, etc.) for reliable guidance of SBRT. Provide a comprehensive overview of emerging digital linacs and summarize the key geometric and dosimetric features of the new generation of linacs for substantially improved SBRT. Discuss treatment planning and quality assurance issues specific to SBRT. Research grant from Varian Medical Systems.

  12. Pono tibi istam: “Tu curris”.Uno sguardo alla teoria medievale delle obbligazioni

    Directory of Open Access Journals (Sweden)

    Riccardo Strobino

    2008-12-01

    Full Text Available La teoria delle obbligazioni rappresenta un significativo esempio di come un insieme di tecniche logiche possa essere applicato, in maniera feconda, a contesti disputazionali in cui la dimensione dialogica e l’interazione tra soggetti coinvolti in un confronto dialettico hanno un ruolo di primo piano. L’attenzione che numerosi studiosi hanno manifestato nei confronti di questa parte della logica modernorum negli ultimi quarat’anni testimonia la profondità e la difficoltà dei problemi teorici che stanno alla base della teoria. Molte interpretazioni sono state avanzate circa le motivazioni che spingono i logici medievali a scrivere trattati in cui il portato delle loro dottrine logiche – come, ad esempio, la teoria delle consequentiae - viene declinato ed elaboratamente applicato al gioco dialettico tra due disputanti. La gamma di tali letture comprende differenti ipotesi: la teoria delle obbligazioni potrebbe fornire un nucleo di regole per l’impostazione di un metodo assiomatico rudimentale così come rappresentare un tentativo di elaborare esercizi volti a valutare le capacità logiche degli studenti o, ancora, un gioco teorico, svincolato dall’effettiva applicazione a contesti concreti, la cui finalità sarebbe il mantenimento della coerenza logica. E’ stata sostenuta da più interpreti la funzione pedagogica svolta dalla teoria, al di là del fatto che si tratti o meno di un metodo per la conduzione effettiva di dispute reali. Un’altra linea interpretativa collega la teoria delle obbligazioni al contesto della letteratura dei sophismata e degli insolubilia, dal cui repertorio, in effetti, sono spesso tratti molti degli esempi che si incontrano nella lettura dei trattati de obligationibus. In questo senso, la teoria offrirebbe regole e schemi di risposta che un interlocutore deve utilizzare per risolvere o rispondere a enunciati problematici o paradossali. Non si può escludere, inoltre, che nella teoria si realizzi un intreccio delle differenti motivazioni sopra esposte: potrebbe trattarsi di uno strumento che viene sviluppato tanto per la risoluzione di sophismata e insolubilia quanto per indagare il rapporto tra linguaggio e metalinguaggio o altre questioni teoriche. Altri studiosi hanno infine sostenuto  che lo scopo della teoria delle obbligazioni consisterebbe nell’elaborazione di un metodo adatto alla costruzione e alla discussione di esperimenti mentali e che questo costituirebbe una sorta di metametodologia della scienza medievale sulla linea del ragionamento secundum imaginationem.

  13. Et tu, Brute? Not Even Intracellular Mutualistic Symbionts Escape Horizontal Gene Transfer

    Directory of Open Access Journals (Sweden)

    Sergio López-Madrigal

    2017-09-01

    Full Text Available Many insect species maintain mutualistic relationships with endosymbiotic bacteria. In contrast to their free-living relatives, horizontal gene transfer (HGT has traditionally been considered rare in long-term endosymbionts. Nevertheless, meta-omics exploration of certain symbiotic models has unveiled an increasing number of bacteria-bacteria and bacteria-host genetic transfers. The abundance and function of transferred loci suggest that HGT might play a major role in the evolution of the corresponding consortia, enhancing their adaptive value or buffering detrimental effects derived from the reductive evolution of endosymbionts’ genomes. Here, we comprehensively review the HGT cases recorded to date in insect-bacteria mutualistic consortia, and discuss their impact on the evolutionary success of these associations.

  14. TU-H-BRB-01: Physics and Dosimetry for Radiation Countermeasure Research.

    Science.gov (United States)

    Bourland, J

    2016-06-01

    The US government has substantial research and development activities underway for medical countermeasures that will insure the long-term safety and survival of the country's population after unfortunate large-scale biological, chemical and radiological and nuclear events. Preparedness includes research and development of medical countermeasures to address radiation-induced cutaneous and internal injury from radiation and nuclear events as well as for minimizing radiation risks incurred during and after travel in space. Other important research and development efforts include the repurposing of countermeasures and development of radioprotectors and mitigators to improve the outcome of radiation treatment. Participating agencies include NIAID, BARDA, NCI, and NASA, with examples of research and development funding that includes the Centers for Medical Countermeasures against Radiation (CMCR) consortia (NIAID) and primary and sub-contracts with commercial entities (BARDA). Each of these programs requires substantial medical and health physics effort in collaboration with biology colleagues to provide a range of radiation sources, dosimetry instrumentation and assessment methods, and animal models for specific radiation-induced effects and injuries. Radiation countermeasure activities for government agencies will be reviewed, the importance of model development will be stressed, example radiation countermeasure research projects will be reviewed, and the roles for medical physicists will be discussed.

  15. TU-CD-BRB-12: Radiogenomics of MRI-Guided Prostate Cancer Biopsy Habitats

    Energy Technology Data Exchange (ETDEWEB)

    Stoyanova, R; Lynne, C; Abraham, S; Patel, M; Jorda, M; Kryvenko, O; Ishkanian, A; Abramowitz, M; Pollack, A [University of Miami, Miami, FL (United States); Tachar, M; Erho, N; Buerki, C; Lam, L; Davicioni, E [GenomeDx Biosciences Inc., Vancouver, British Columbia (Canada)

    2015-06-15

    Purpose: Diagnostic prostate biopsies are subject to sampling bias. We hypothesize that quantitative imaging with multiparametric (MP)-MRI can more accurately direct targeted biopsies to index lesions associated with highest risk clinical and genomic features. Methods: Regionally distinct prostate habitats were delineated on MP-MRI (T2-weighted, perfusion and diffusion imaging). Directed biopsies were performed on 17 habitats from 6 patients using MRI-ultrasound fusion. Biopsy location was characterized with 52 radiographic features. Transcriptome-wide analysis of 1.4 million RNA probes was performed on RNA from each habitat. Genomics features with insignificant expression values (<0.25) and interquartile range <0.5 were filtered, leaving total of 212 genes. Correlation between imaging features, genes and a 22 feature genomic classifier (GC), developed as a prognostic assay for metastasis after radical prostatectomy was investigated. Results: High quality genomic data was derived from 17 (100%) biopsies. Using the 212 ‘unbiased’ genes, the samples clustered by patient origin in unsupervised analysis. When only prostate cancer related genomic features were used, hierarchical clustering revealed samples clustered by needle-biopsy Gleason score (GS). Similarly, principal component analysis of the imaging features, found the primary source of variance segregated the samples into high (≥7) and low (6) GS. Pearson’s correlation analysis of genes with significant expression showed two main patterns of gene expression clustering prostate peripheral and transitional zone MRI features. Two-way hierarchical clustering of GC with radiomics features resulted in the expected groupings of high and low expressed genes in this metastasis signature. Conclusions: MP-MRI-targeted diagnostic biopsies can potentially improve risk stratification by directing pathological and genomic analysis to clinically significant index lesions. As determinant lesions are more reliably identified, targeting with radiotherapy should improve outcome. This is the first demonstration of a link between quantitative imaging features (radiomics) with genomic features in MRI-directed prostate biopsies. The research was supported by NIH- NCI R01 CA 189295 and R01 CA 189295; E Davicioni is partial owner of GenomeDx Biosciences, Inc. M Takhar, N Erho, L Lam, C Buerki and E Davicioni are current employees at GenomeDx Biosciences, Inc.

  16. Ovlasti i obveze upravnog suca pri pretresu tužbe

    OpenAIRE

    Massot, Jean

    2010-01-01

    Za prikazati što je specifično „dužnosti upravnog suca“ u zemljama kao što je Francuska, treba se osvrnuti na povijesni i zemljopisni prikaz. Izlaže se razloge postojanja upravnog suca u velikom broju demokratskih zemalja i onih koji koračaju prema demokraciji. Ističu se različitosti i specifičnosti upravnog suca u odnosu na ostale suce.

  17. TU-F-BRB-03: Clinical Implementation of MR-Based Motion Management

    Energy Technology Data Exchange (ETDEWEB)

    Glide-Hurst, C. [Henry Ford Health System (United States)

    2015-06-15

    The current clinical standard of organ respiratory imaging, 4D-CT, is fundamentally limited by poor soft-tissue contrast and imaging dose. These limitations are potential barriers to beneficial “4D” radiotherapy methods which optimize the target and OAR dose-volume considering breathing motion but rely on a robust motion characterization. Conversely, MRI imparts no known radiation risk and has excellent soft-tissue contrast. MRI-based motion management is therefore highly desirable and holds great promise to improve radiotherapy of moving cancers, particularly in the abdomen. Over the past decade, MRI techniques have improved significantly, making MR-based motion management clinically feasible. For example, cine MRI has high temporal resolution up to 10 f/s and has been used to track and/or characterize tumor motion, study correlation between external and internal motions. New MR technologies, such as 4D-MRI and MRI hybrid treatment machines (i.e. MR-linac or MR-Co60), have been recently developed. These technologies can lead to more accurate target volume determination and more precise radiation dose delivery via direct tumor gating or tracking. Despite all these promises, great challenges exist and the achievable clinical benefit of MRI-based tumor motion management has yet to be fully explored, much less realized. In this proposal, we will review novel MR-based motion management methods and technologies, the state-of-the-art concerning MRI development and clinical application and the barriers to more widespread adoption. Learning Objectives: Discuss the need of MR-based motion management for improving patient care in radiotherapy. Understand MR techniques for motion imaging and tumor motion characterization. Understand the current state of the art and future steps for clinical integration. Henry Ford Health System holds research agreements with Philips Healthcare. Research sponsored in part by a Henry Ford Health System Internal Mentored Grant.

  18. TU-PIS-Exhibit Hall-2: How to Move Beyond Dose Monitoring to Imaging Performance Utilization

    Energy Technology Data Exchange (ETDEWEB)

    Valencia, D. [Imalogix (United States)

    2015-06-15

    The current clinical standard of organ respiratory imaging, 4D-CT, is fundamentally limited by poor soft-tissue contrast and imaging dose. These limitations are potential barriers to beneficial “4D” radiotherapy methods which optimize the target and OAR dose-volume considering breathing motion but rely on a robust motion characterization. Conversely, MRI imparts no known radiation risk and has excellent soft-tissue contrast. MRI-based motion management is therefore highly desirable and holds great promise to improve radiotherapy of moving cancers, particularly in the abdomen. Over the past decade, MRI techniques have improved significantly, making MR-based motion management clinically feasible. For example, cine MRI has high temporal resolution up to 10 f/s and has been used to track and/or characterize tumor motion, study correlation between external and internal motions. New MR technologies, such as 4D-MRI and MRI hybrid treatment machines (i.e. MR-linac or MR-Co60), have been recently developed. These technologies can lead to more accurate target volume determination and more precise radiation dose delivery via direct tumor gating or tracking. Despite all these promises, great challenges exist and the achievable clinical benefit of MRI-based tumor motion management has yet to be fully explored, much less realized. In this proposal, we will review novel MR-based motion management methods and technologies, the state-of-the-art concerning MRI development and clinical application and the barriers to more widespread adoption. Learning Objectives: Discuss the need of MR-based motion management for improving patient care in radiotherapy. Understand MR techniques for motion imaging and tumor motion characterization. Understand the current state of the art and future steps for clinical integration. Henry Ford Health System holds research agreements with Philips Healthcare. Research sponsored in part by a Henry Ford Health System Internal Mentored Grant.

  19. TU-EF-304-04: A Heart Motion Model for Proton Scanned Beam Chest Radiotherapy

    Energy Technology Data Exchange (ETDEWEB)

    White, B; Kiely, J Blanco; Lin, L; Freedman, G; Both, S [University of Pennsylvania, Philadelphia, PA (United States); Vennarini, S [Operativa di Protonterapia, Azienda Provinciale per i Servizi Sanitari, Trento, Trento (Italy); Santhanam, A; Low, D [University of California, Los Angeles, Los Angeles, CA (United States)

    2015-06-15

    Purpose: To model fast-moving heart surface motion as a function of cardiac-phase in order to compensate for the lack of cardiac-gating in evaluating accurate dose to coronary structures. Methods: Ten subjects were prospectively imaged with a breath-hold, cardiac-gated MRI protocol to determine heart surface motion. Radial and planar views of the heart were resampled into a 3-dimensional volume representing one heartbeat. A multi-resolution optical flow deformable image registration algorithm determined tissue displacement during the cardiac-cycle. The surface of the heart was modeled as a thin membrane comprised of voxels perpendicular to a pencil beam scanning (PBS) beam. The membrane’s out-of-plane spatial displacement was modeled as a harmonic function with Lame’s equations. Model accuracy was assessed with the root mean squared error (RMSE). The model was applied to a cohort of six chest wall irradiation patients with PBS plans generated on phase-sorted 4DCT. Respiratory motion was separated from the cardiac motion with a previously published technique. Volumetric dose painting was simulated and dose accumulated to validate plan robustness (target coverage variation accepted within 2%). Maximum and mean heart surface dose assessed the dosimetric impact of heart and coronary artery motion. Results: Average and maximum heart surface displacements were 2.54±0.35mm and 3.6mm from the end-diastole phase to the end-systole cardiac-phase respectively. An average RMSE of 0.11±0.04 showed the model to be accurate. Observed errors were greatest between the circumflex artery and mitral valve level of the heart anatomy. Heart surface displacements correspond to a 3.6±1.0% and 5.1±2.3% dosimetric impact on the maximum and mean heart surface DVH indicators respectively. Conclusion: Although heart surface motion parallel to beam’s direction was substantial, its maximum dosimetric impact was 5.1±2.3%. Since PBS delivers low doses to coronary structures relative to photon radiotherapy, it is unknown whether this variation would be clinically significant for late effects.

  20. TU-A-304-02: Treatment Simulation, Planning and Delivery for SBRT

    Energy Technology Data Exchange (ETDEWEB)

    Yang, Y.

    2015-06-15

    Increased use of SBRT and hypo fractionation in radiation oncology practice has posted a number of challenges to medical physicist, ranging from planning, image-guided patient setup and on-treatment monitoring, to quality assurance (QA) and dose delivery. This symposium is designed to provide updated knowledge necessary for the safe and efficient implementation of SBRT in various linac platforms, including the emerging digital linacs equipped with high dose rate FFF beams. Issues related to 4D CT, PET and MRI simulations, 3D/4D CBCT guided patient setup, real-time image guidance during SBRT dose delivery using gated/un-gated VMAT or IMRT, and technical advancements in QA of SBRT (in particular, strategies dealing with high dose rate FFF beams) will be addressed. The symposium will help the attendees to gain a comprehensive understanding of the SBRT workflow and facilitate their clinical implementation of the state-of-art imaging and planning techniques. Learning Objectives: Present background knowledge of SBRT, describe essential requirements for safe implementation of SBRT, and discuss issues specific to SBRT treatment planning and QA. Update on the use of multi-dimensional (3D and 4D) and multi-modality (CT, beam-level X-ray imaging, pre- and on-treatment 3D/4D MRI, PET, robotic ultrasound, etc.) for reliable guidance of SBRT. Provide a comprehensive overview of emerging digital linacs and summarize the key geometric and dosimetric features of the new generation of linacs for substantially improved SBRT. Discuss treatment planning and quality assurance issues specific to SBRT. Research grant from Varian Medical Systems.