WorldWideScience

Sample records for plant transformation service

  1. Transformative service design

    DEFF Research Database (Denmark)

    Sørensen, Kirsten Bonde

    2014-01-01

    -behaviour’. These handmade, artistic strategies turned out to be highly self-persuasive: the majority of the participants changed their perception and behaviour in relation to money. - See more at: http://www.service-design-network.org/products-page/article/tp05-3p54/#sthash.3MX79Wx6.dpuf...

  2. PLANT TRANSFORMATION: ADVANCES AND PERSPECTIVES

    Directory of Open Access Journals (Sweden)

    Alves Adriana Cristina

    1999-01-01

    Full Text Available Genetic transformation is a powerful tool for plant breeding and genetical, physiological or biochemical research, consequently it is an extremely dynamic field. Transgenic plants are commonly used to complete or substitute mutants in basic research, helping the studies of complex biological situations such as pathogenesis process, genome organization, light reception and signal transduction. In this review, recent approaches for foreign gene introduction (e.g. Agrobiolistics, whole tissue electroporation, in planta Agrobacterium transformation, screening (reporter gene possibilities and performance and transformant selection (ipt selective marker are discussed. Transgene expression and mechanisms underlying (transgene inactivation are presented. Practical applications of genetically modified plants, field tests and commercial transgenic crops worldwide and in Brazil are listed, as well as the main traits and species modified. Potential uses of transgenic plants for animal compound production, biological remediation and synthetic polymer assembly are also shown.

  3. Transformative service design

    DEFF Research Database (Denmark)

    Sørensen, Kirsten Bonde

    2014-01-01

    At ‘The MoneyWorkshop’ — a creative workshop conducted at a Danish bank in 2011 — bank customers were invited to have artful and creative dialogues with themselves, cutting and pasting images of their present perceptions about money as well as strategies on their desired ‘money-behaviour’. These ......At ‘The MoneyWorkshop’ — a creative workshop conducted at a Danish bank in 2011 — bank customers were invited to have artful and creative dialogues with themselves, cutting and pasting images of their present perceptions about money as well as strategies on their desired ‘money......-behaviour’. These handmade, artistic strategies turned out to be highly self-persuasive: the majority of the participants changed their perception and behaviour in relation to money. - See more at: http://www.service-design-network.org/products-page/article/tp05-3p54/#sthash.3MX79Wx6.dpuf...

  4. Micro-shock Wave Assisted Plant Transformation

    Science.gov (United States)

    Gnanadhas, Divya Prakash; Datey, Akshay; Chakravortty, Dipshikha; Gopalan, Jagadeesh

    Genetically modified (GM) crops are developed by transforming the desired DNA to plant. There are various methods employed to achieve the required transformation in plants. Agrobacterium mediated transformation and Biolistics or particle bombardment method are the most commonly used methods.

  5. Physical methods for genetic plant transformation

    Science.gov (United States)

    Rivera, Ana Leonor; Gómez-Lim, Miguel; Fernández, Francisco; Loske, Achim M.

    2012-09-01

    Production of transgenic plants is a routine process for many crop species. Transgenes are introduced into plants to confer novel traits such as improved nutritional qualities, tolerance to pollutants, resistance to pathogens and for studies of plant metabolism. Nowadays, it is possible to insert genes from plants evolutionary distant from the host plant, as well as from fungi, viruses, bacteria and even animals. Genetic transformation requires penetration of the transgene through the plant cell wall, facilitated by biological or physical methods. The objective of this article is to review the state of the art of the physical methods used for genetic plant transformation and to describe the basic physics behind them.

  6. Transformation of medicinal plants using Agrobacterium tumefaciens.

    Science.gov (United States)

    Bandurska, Katarzyna; Berdowska, Agnieszka; Król, Małgorzata

    2016-12-20

    For many years attempts are made to develop efficient methods for transformation of medicinal plants via Agrobacterium tumefaciens. It is a soil bacteria which possess a natural ability to infect plants in places of injures which results in arise of cancerous growths (crown gall). This is possible thanks a transfer of fragment of Ti plasmid into plant cells and stable integration with a plant genome. Efficiency of medicinal plant transformation depends on many factors for example: Agrobacterium strain, methods and procedures of transformation as well as on plant species, type and age of the explants and regeneration conditions. The main goal of plant transformation is to increase the amount of naturally occurring bioactive compounds and the production of biopharmaceuticals. Genetic plant transformation via bacteria of the genus Agrobacterium is a complex process which requires detailed analysis of incorporated transgene expression and occurs only in the case when the plant cell acquires the ability to regenerate. In many cases, the regeneration efficiency observed in medicinal plants are inefficient after applied transformation procedures. To date there have been attempts of genetic transformation by using A. tumefaciens of medicinal plants belonging to the families: Apocynaceae, Araceae, Araliaceae, Asphodelaceae, Asteraceae, Begoniaceae, Crassulaceae, Fabaceae, Lamiaceae, Linaceae, Papaveraceae, Plantaginaceae, Scrophulariaceae and Solanaceae.

  7. Wind power plant system services

    DEFF Research Database (Denmark)

    Basit, Abdul; Altin, Müfit

    Traditionally, conventional power plants have the task to support the power system, by supplying power balancing services. These services are required by the power system operators in order to secure a safe and reliable operation of the power system. However, as in the future the wind power...... is going more and more to replace conventional power plants, the sources of conventional reserve available to the system will be reduced and fewer conventional plants will be available on-line to share the regulation burden. The reliable operation of highly wind power integrated power system might...... then beat risk unless the wind power plants (WPPs) are able to support and participate in power balancing services. The objective of this PhD project is to develop and analyse control strategies which can increase the WPPs capability to provide system services, such as active power balancing control...

  8. Transformation of plant young proembryos by electroporation

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    It is first reported that plant young proembryos expressed exogenous reporter genes by electroporation. Young proembryos with 8-32 cells and globular proembryos with 250-400 cells could be isolated by enzymatic maceration combined with microdissection. After electroporation with GUS or GFP genes, the proembryos were cultured for 1-2 d in KM8p medium. At the field strength of electroporation 500-1 500 V/cm, blue reaction of GUS or green fluorescence of GFP could be observed in the proembryos. The highest transient expression frequency of young proembryos (2.2%) was obtained at the field strength of 750 V/cm, whereas the highest frequency of globular proembryos (5.9%) was obtained at the field strength of 1 250 V/cm. Taking the proportion of transformed cells in the whole cells of proembryos as efficient transformation frequency, the efficient transformation frequency of the young proembryos was 7 times that of the globular proembryos.

  9. US Forest Service Current Invasive Plants Inventory

    Data.gov (United States)

    US Forest Service, Department of Agriculture — A map service on the www depicting the most recent measurement of Invasive Plant Infestation polygons collected by the National Invasive Plant Inventory Protocol....

  10. Recovery in England: transforming statutory services?

    Science.gov (United States)

    Perkins, Rachel; Slade, Mike

    2012-02-01

    English mental health policy has explicitly supported a focus on recovery since 2001. More recently, this has been elaborated through policy support for social inclusion, employment and well-being. We review several drivers for this political orientation, including a refocusing of the role of health services as a whole from treating illnesses to helping people to make the most of their lives, the shift to greater power for the individual, reflected in personal social care and personal health budgets, and the evidence informing clinical guidelines issued by the National Institute for Health and Clinical Excellence (NICE). A disjunction remains between policy and practice, with organizational policies espousing a recovery orientation and teams re-branding as 'recovery and support' teams, whilst pursuing clinical practices which prioritize symptomatic treatment rather than recovery support. The next phase of development in English statutory mental health services is therefore bridging this gap, through organizational transformation in mental health services towards a focus on recovery. We describe two funded initiatives to support this process of organizational transformation. The first (ImROC) is a national initiative to develop a pro-recovery organizational climate. The second (REFOCUS) is a multi-site cluster randomized controlled trial (ISRCTN02507940) investigating a team-level pro-recovery intervention.

  11. Atmospheric transformation of plant volatiles disrupts host plant finding

    Science.gov (United States)

    Li, Tao; Blande, James D.; Holopainen, Jarmo K.

    2016-09-01

    Plant-emitted volatile organic compounds (VOCs) play important roles in plant-insect interactions. Atmospheric pollutants such as ozone (O3) can react with VOCs and affect the dynamics and fidelity of these interactions. However, the effects of atmospheric degradation of plant VOCs on plant-insect interactions remains understudied. We used a system comprising Brassica oleracea subsp. capitata (cabbage) and the specialist herbivore Plutella xylostella to test whether O3-triggered VOC degradation disturbs larval host orientation, and to investigate the underlying mechanisms. Larvae oriented towards both constitutive and larva-induced cabbage VOC blends, the latter being the more attractive. Such behaviour was, however, dramatically reduced in O3-polluted environments. Mechanistically, O3 rapidly degraded VOCs with the magnitude of degradation increasing with O3 levels. Furthermore, we used Teflon filters to collect VOCs and their reaction products, which were used as odour sources in behavioural tests. Larvae avoided filters exposed to O3-transformed VOCs and spent less time searching on them compared to filters exposed to original VOCs, which suggests that some degradation products may have repellent properties. Our study clearly demonstrates that oxidizing pollutants in the atmosphere can interfere with insect host location, and highlights the need to address their broader impacts when evaluating the ecological significance of VOC-mediated interactions.

  12. Plant regeneration and genetic transformation in Jatropha

    KAUST Repository

    Sujatha, M.

    2012-07-01

    Jatropha curcas, a non-edible oil bearing species with multiple uses, and considerable economic potential is emerging as a potential biofuel plant. The limited knowledge of this species, low and inconsistent yields, the narrow genetic variability, and vulnerability to insects and diseases are major constraints in successful cultivation of Jatropha as a biofuel crop. Hence, genetic improvement of Jatropha is essential by conventional and modern biotechnological tools to use as a viable alternative source of bio-diesel. Realising its potential as a bio-energy crop, in vitro regeneration methods have been established to meet the demand of large scale supply of superior clones, and also as a prelude for genetic improvement of the species through transgenic approaches. In this chapter, an overview of in vitro tissue culture and genetic transformation of Jatropha is discussed. © 2013 Springer Science+Business Media Dordrecht. All rights are reserved.

  13. Transforming Service Employees and Climate: A Multilevel, Multisource Examination of Transformational Leadership in Building Long-Term Service Relationships

    Science.gov (United States)

    Liao, Hui; Chuang, Aichia

    2007-01-01

    This longitudinal field study integrates the theories of transformational leadership (TFL) and relationship marketing to examine how TFL influences employee service performance and customer relationship outcomes by transforming both (at the micro level) the service employees' attitudes and (at the macro level) the work unit's service climate.…

  14. Transforming Service Employees and Climate: A Multilevel, Multisource Examination of Transformational Leadership in Building Long-Term Service Relationships

    Science.gov (United States)

    Liao, Hui; Chuang, Aichia

    2007-01-01

    This longitudinal field study integrates the theories of transformational leadership (TFL) and relationship marketing to examine how TFL influences employee service performance and customer relationship outcomes by transforming both (at the micro level) the service employees' attitudes and (at the macro level) the work unit's service climate.…

  15. Exploring the Unknown: International Service and Individual Transformation

    Science.gov (United States)

    Chang, Wei-Wen; Chen, Cheng-Hui Lucy; Huang, Yu-Fu; Yuan, Yu-Hsi

    2012-01-01

    Empirical studies have found that participation in international service increases learners' intercultural competence, language skills, appreciation of cultural differences, and tolerance for ambiguity. While previous studies suggest that international service experience is potentially transformative in nature, the present study examined…

  16. Innovations in plant health services in Nicaragua

    DEFF Research Database (Denmark)

    Danielsen, Solveig; Centeno, Julio; López, Julio

    2013-01-01

    Establishing a few community-based plant clinics in Nicaragua led to a series of innovations in plant health service delivery. A grassroots experiment became a nationwide initiative involving local service providers, universities, research institutions and diagnostic laboratories. This led to the...

  17. DETERMINATION OF REPLACEMENT EFFICIENCY OF TRANSFORMERS WITH LONG SERVICE LIFE

    Directory of Open Access Journals (Sweden)

    M. I. Fursanov

    2014-01-01

    Full Text Available The paper shows non-efficient application of the existing economical models for determination of replacement time for transformers with long service life because these models do not take into account real changes in transformer parameters during its operational period. An improved mathematical model for estimation of replacement efficiency of power transformers which are used at substations of power distribution networks has been developed in the paper. The model makes it possible to determine an optimum replacement time and economically justified operational service life of the transformers.

  18. Transformation and regeneration of the holoparasitic plant Phelipanche aegyptiaca

    Directory of Open Access Journals (Sweden)

    Fernández-Aparicio Mónica

    2011-11-01

    Full Text Available Abstract Background Transformation and subsequent regeneration of holoparasitic plants has never been reported, in part due to challenges in developing transformation protocols, but also because regeneration of obligate parasites is difficult since their survival depends completely on successful haustorium penetration of a host and the formation of vascular connections. The recent completion of a massive transcriptome sequencing project (the Parasitic Plant Genome Project will fuel the use of genomic tools for studies on parasitic plants. A reliable system for holoparasite transformation is needed to realize the full value of this resource for reverse genetics and functional genomics studies. Results Here we demonstrate that transformation of Phelipanche aegyptiaca is achieved by infection of 3 month-old in vitro grown P. aegyptiaca calli with Agrobacterium rhizogenes harboring the yellow fluorescent protein (YFP. Four months later, YFP-positive regenerated calli were inoculated onto tomato plants growing in a minirhizotron system. Eight days after inoculation, transgenic parasite tissue formed lateral haustoria that penetrated the host and could be visualized under UV illumination through intact host root tissue. YFP-positive shoot buds were observed one month after inoculation. Conclusions This work constitutes a breakthrough in holoparasitic plant research methods. The method described here is a robust system for transformation and regeneration of a holoparasitic plant and will facilitate research on unique parasitic plant capabilities such as host plant recognition, haustorial formation, penetration and vascular connection.

  19. Service Blueprinting: Transforming the Student Experience

    Science.gov (United States)

    Bitner, Mary Jo; Ostrom, Amy L.; Burkhard, Kevin A.

    2012-01-01

    There is much discussion today about the need to transform higher education for the benefit of students, employers, and society at large. Experts and researchers list the numerous challenges: low student retention and graduation rates, the increasing cost of higher education, and concerns that graduates don't possess the skills required to compete…

  20. Evidences of Transformative Learning in Service-Learning Reflections

    Science.gov (United States)

    Hullender, Ren; Hinck, Shelly; Wood-Nartker, Jeanneane; Burton, Travus; Bowlby, Sue

    2015-01-01

    Service-learning environments are complex learning contexts that generate a level of disequilibrium or anxiety that may or may not result in transformative learning. This phenomenological study examined student reflective writings from an Honors service-learning course at a medium-sized mid-western university for evidences of transformative…

  1. transforming agriculture through contracted extension service ...

    African Journals Online (AJOL)

    ACSS

    Agribusiness Project (KAPAP) is implementing an innovative service delivery model, whose approaches include ... include high level value chain interventions such as organising farmers for marketing, and linking them to markets .... KAPP 1, were taken into account in the new .... barazas to create awareness on the Project.

  2. VIP1: linking Agrobacterium-mediated transformation to plant immunity?

    Science.gov (United States)

    Liu, Yukun; Kong, Xiangpei; Pan, Jiaowen; Li, Dequan

    2010-08-01

    Agrobacterium tumefaciens is the most efficient vehicle used today for the production of transgenic plants and plays an essential role in basic scientific research and in agricultural biotechnology. Previously, plant VirE2-interacting protein 1 (VIP1) was shown to play a role in Agrobacterium-mediated transformation. Recent reports demonstrate that VIP1, as one of the bZIP transcription factors, is also involved in plant immunity responses. Agrobacterium is able to activate and abuse VIP1 for transformation. These findings highlight Agrobacterium-host interaction and unveil how Agrobacterium hijacks host cellular mechanism for its own benefit. This review focuses on the roles played by VIP1 in Agrobacterium-mediated transformation and plant immunity.

  3. Modern services and China’s economic transformation

    Institute of Scientific and Technical Information of China (English)

    何德旭; 姚战琪

    2008-01-01

    Development of a modern service industry must be accelerated if China’s economic growth pattern is to be transformed and its industrial structure upgraded. For a long time, China’s modern services have been backward and played an insignificant role in the national economy. Sluggish growth of modern services is particularly dragging on China’s otherwise surging economic and social development; and it puts a strain on industrial restructuring and efforts to build a prosperous and harmonious socialist society. This paper sheds light on outstanding issues concerning the role of China’s modern services in various arenas, including transforming the economic growth pattern, improving economic structuring, upgrading the industrial structure, facilitating technological progress and spurring changes in the employment structure. Based on in-depth analysis, this essay proposes policies and countermeasures for the promotion of modern services.

  4. Genetic transformation of plants by protoplast electroporation.

    Science.gov (United States)

    Bates, G W

    1994-10-01

    This article describes an optimized protocol for the electroporation of tobacco mesophyll protoplasts together with notes and data on the effects of various parameters and suggestions for work with protoplasts of other species. In this protocol, electroporation is achieved by means of electrical pulses from a high-voltage, capacitive-discharge unit. Procedures are described for measurement of protoplast viability with Evan's blue, the detection of transient expression of CAT and GUS gene plasmid constructs, and for the recovery of stable transformants based on selection for kanamycin resistance.

  5. Cold tolerance of potato plants transformed with yeast invertase gene

    Directory of Open Access Journals (Sweden)

    Alexander N. Deryaabin

    2013-12-01

    Full Text Available Our study was carried out with potato plants (Solanum tuberosun L.,cv. Désirée transformed with the yeast invertase gene under the control of the B33 class I patatin promoter and with the proteinase inhibitor II leader peptide sequence providing for the apoplastic enzyme localization (B33-inv plants and with the plants transformed with the reporter gene encoding bb-glucuronidase under the control of the 35S CaMV promoter (control plants. Exposure to 5°C during 6 days caused an increase in invertase activity and sugar content in B33-inv leaves in comparison with the control plants. Cell membranes of B33-inv plant cells showed greater cold tolerance under low temperature conditions than control plants that was recorded by electrolyte release. We supposed that higher cold tolerance of B33-inv plants was caused by stabilizing effect of sugar on the membranes, because B33-inv plants differ from the control plants in higher invertase activity, induced by expression of yeast invertase gene, and high content of sugars.

  6. Plant regeneration and genetic transformation in switchgrass-A review

    Institute of Scientific and Technical Information of China (English)

    Paul Merrick; Shuizhang Fei

    2015-01-01

    Switchgrass is native to the tal grass prairie of North America. It is self-incompatible and has varied ploidy levels from diploid (2x) to dodecaploid (12x) with tetraploid and octoploid being the most common. The high yielding potential and the ability to grow wel in marginal lands make switchgrass an ideal species as a dedicated biomass producer for lignocel ulosic ethanol production. Genetic transformation is an important tool for studying gene function and for germplasm improvement in switchgrass, the genome of which has been sequenced recently. This paper intends to provide a comprehensive review on plant regeneration and genetic transformation in switchgrass. We ifrst reviewed the effect of explants, basal medium and plant growth regulators on plant regeneration in switchgrass, which is a prerequisite for genetic transformation. We then reviewed the progresses on genetic transformation with either the biolistic or Agrobacterium-mediated method in switchgrass, and discussed various techniques employed to improve the transformation efifciency. Final y we reviewed the recent progresses on the use of genetic transformation in improving biomass quality such as the reduction of lignin, and in increasing biomass yield in switchgrass. We also provided a future perspective on the use of new genome editing technologies in switchgrass and its potential impact on regulatory processes.

  7. Adaptation services of floodplains and wetlands under transformational climate change.

    Science.gov (United States)

    Colloff, Matthew; Lavorel, Sandra; Wise, Russell M; Dunlop, Michael; Overton, Ian C; Williams, Kristen J

    2016-06-01

    Adaptation services are the ecosystem processes and services that benefit people by increasing their ability to adapt to change. Benefits may accrue from existing but newly used services where ecosystems persist or from novel services supplied following ecosystem transformation. Ecosystem properties that enable persistence or transformation are important adaptation services because they support future options. The adaptation services approach can be applied to decisions on trade-offs between currently valued services and benefits from maintaining future options. For example, ecosystem functions and services of floodplains depend on river flows. In those regions of the world where climate change projections are for hotter, drier conditions, floods will be less frequent and floodplains will either persist, though with modified structure and function, or transform to terrestrial (flood-independent) ecosystems. Many currently valued ecosystem services will reduce in supply or become unavailable, but new options are provided by adaptation services. We present a case study from the Murray-Darling Basin, Australia, for operationalizing the adaptation services concept for floodplains and wetlands. We found large changes in flow and flood regimes are likely under a scenario of +1.6°C by 2030, even with additional water restored to rivers under the proposed Murray-Darling Basin Plan. We predict major changes to floodplain ecosystems, including contraction of riparian forests and woodlands and expansion of terrestrial, drought-tolerant vegetation communities. Examples of adaptation services under this scenario include substitution of irrigated agriculture with dryland cropping and floodplain grazing; mitigation of damage from rarer, extreme floods; and increased tourism, recreational, and cultural values derived from fewer, smaller wetlands that can be maintained with environmental flows. Management for adaptation services will require decisions on where intervention can

  8. Architectural Decision Management for Digital Transformation of Products and Services

    Directory of Open Access Journals (Sweden)

    Alfred Zimmermann

    2016-04-01

    Full Text Available The digitization of our society changes the way we live, work, learn, communicate, and collaborate. The Internet of Things, Enterprise Social Networks, Adaptive Case Management, Mobility systems, Analytics for Big Data, and Cloud services environments are emerging to support smart connected products and services and the digital transformation. Biological metaphors of living and adaptable ecosystems provide the logical foundation for self-optimizing and resilient run-time environments for intelligent business services and service-oriented enterprise architectures. Our aim is to support flexibility and agile transformations for both business domains and related information technology. The present research paper investigates mechanisms for decision analytics in the context of multi-perspective explorations of enterprise services and their digital enterprise architectures by extending original architecture reference models with state of art elements for agile architectural engineering for the digitization and collaborative architectural decision support. The paper’s context focuses on digital transformations of business and IT and integrates fundamental mappings between adaptable digital enterprise architectures and service-oriented information systems. We are putting a spotlight on the example domain – Internet of Things.

  9. Novel and potential application of cryopreservation to plant genetic transformation.

    Science.gov (United States)

    Wang, Biao; Zhang, Zhibo; Yin, Zhenfang; Feng, Chaohong; Wang, Qiaochun

    2012-01-01

    The world population now is 6.7 billion and is predicted to reach 9 billion by 2050. Such a rapid growing population has tremendously increased the challenge for food security. Obviously, it is impossible for traditional agriculture to ensure the food security, while plant biotechnology offers considerable potential to realize this goal. Over the last 15 years, great benefits have been brought to sustainable agriculture by commercial cultivation of genetically modified (GM) crops. Further development of new GM crops will with no doubt contribute to meeting the requirements for food by the increasing population. The present article provides updated comprehensive information on novel and potential application of cryopreservation to genetic transformation. The major progresses that have been achieved in this subject include (1), long-term storage of a large number of valuable plant genes, which offers a good potential for further development of novel cultivars by genetic transformation; (2), retention of regenerative capacity of embryogenic tissues and protoplasts, which ensures efficient plant regeneration system for genetic transformation; (3), improvement of transformation efficiency and plant regeneration of transformed cells; (4), long-term preservation of transgenic materials with stable expression of transgenes and productive ability of recombinant proteins, which allows transgenic materials to be stored in a safe manner before being analyzed and evaluated, and allows establishment of stable seed stocks for commercial production of homologous proteins. Data provided in this article clearly demonstrate that cryo-technique has an important role to play in the whole chain of genetic transformation. Further studies coupling cryotechnique and genetic transformation are expected to significantly improve development of new GM crops.

  10. Plant Products for Pharmacology: Application of Enzymes in Their Transformations

    Directory of Open Access Journals (Sweden)

    Marie Zarevúcka

    2008-12-01

    Full Text Available Different plant products have been subjected to detailed investigations due to their increasing importance for improving human health. Plants are sources of many groups of natural products, of which large number of new compounds has already displayed their high impact in human medicine. This review deals with the natural products which may be found dissolved in lipid phase (phytosterols, vitamins etc.. Often subsequent convenient transformation of natural products may further improve the pharmacological properties of new potential medicaments based on natural products. To respect basic principles of sustainable and green procedures, enzymes are often employed as efficient natural catalysts in such plant product transformations. Transformations of lipids and other natural products under the conditions of enzyme catalysis show increasing importance in environmentally safe and sustainable production of pharmacologically important compounds. In this review, attention is focused on lipases, efficient and convenient biocatalysts for the enantio- and regioselective formation / hydrolysis of ester bond in a wide variety of both natural and unnatural substrates, including plant products, eg. plant oils and other natural lipid phase compounds. The application of enzymes for preparation of acylglycerols and transformation of other natural products provides big advantage in comparison with employing of conventional chemical methods: Increased selectivity, higher product purity and quality, energy conservation, elimination of heavy metal catalysts, and sustainability of the employed processes, which are catalyzed by enzymes. Two general procedures are used in the transformation of lipid-like natural products: (a Hydrolysis/alcoholysis of triacylglycerols and (b esterification of glycerol. The reactions can be performed under conventional conditions or in supercritical fluids/ionic liquids. Enzyme-catalyzed reactions in supercritical fluids combine the

  11. Biofuel Potential of Plants Transformed Genetically With NAC Family Genes

    Directory of Open Access Journals (Sweden)

    Sadhana eSingh

    2016-01-01

    Full Text Available NAC genes contribute to enhance survivability of plants under conditions of environmental stress and in secondary growth of the plants, thereby building biomass. Thus, genetic transformation of plants using NAC genes provides a possibility to tailor made biofuel plants. Over-expression studies have indicated that NAC family genes can provide tolerance to various biotic and abiotic stresses, either by physiological or biochemical changes at the cellular level, or by affecting visible morphological and anatomical changes, for example by development of lateral roots in a number of plants. Over-expression of these genes also work as triggers for development of secondary cell walls. In our laboratory, we have observed a NAC gene from Lepidium latifolium contributing to both enhanced biomass as well as cold stress tolerance of model plants tobacco. Thus, we have reviewed all the developments of genetic engineering using NAC genes which could enhance the traits required for biofuel plants, either by enhancing the stress tolerance or by enhancing the biomass of the plants. KeywordsNAC, Genetically engineered plants, Abiotic stress tolerance, Secondary growth, Cell wall synthesis, Biomass

  12. Comparison of Soybean Transformation Efficiency and Plant Factors Affecting Transformation during the Agrobacterium Infection Process.

    Science.gov (United States)

    Jia, Yuying; Yao, Xingdong; Zhao, Mingzhe; Zhao, Qiang; Du, Yanli; Yu, Cuimei; Xie, Futi

    2015-01-01

    The susceptibility of soybean genotype to Agrobacterium infection is a key factor for the high level of genetic transformation efficiency. The objective of this study is to evaluate the plant factors related to transformation in cotyledonary nodes during the Agrobacterium infection process. This study selected three genotypes (Williams 82, Shennong 9 and Bert) with high transformation efficiency, which presented better susceptibility to Agrobacterium infection, and three low transformation efficiency genotypes (General, Liaodou 16 and Kottman), which showed a relatively weak susceptibility. Gibberellin (GA) levels and soybean GA20ox2 and CYP707A2 transcripts of high-efficiency genotypes increased and were higher than those of low-efficiency genotypes; however, the opposite performance was shown in abscisic acid (ABA). Higher zeatin riboside (ZR) content and DNA quantity, and relatively higher expression of soybean IPT5, CYCD3 and CYCA3 were obtained in high-efficiency genotypes. High-efficiency genotypes had low methyl jasmonate (MeJA) content, polyphenol oxidase (PPO) and peroxidase (POD) activity, and relatively lower expression of soybean OPR3, PPO1 and PRX71. GA and ZR were positive plant factors for Agrobacterium-mediated soybean transformation by facilitating germination and growth, and increasing the number of cells in DNA synthesis cycle, respectively; MeJA, PPO, POD and ABA were negative plant factors by inducing defence reactions and repressing germination and growth, respectively.

  13. [PLANT GENETIC TRANSFORMATION USING CARBON NANOTUBES FOR DNA DELIVERY].

    Science.gov (United States)

    Burlaka, O M; Pirko, Ya V; Yemets, A I; Blume, Ya B

    2015-01-01

    The possibility of exploiting carbon nanotubes (CNTs)-based nanocarriers to deliver genes into protoplasts, callus and mesophyll explants of plants was examined. Using single-walled CNTs (SWCNTs) at the concentration of 20 μg/ml and multi-walled CNTs (MWCNTs) at the concentration of 15 μg/ml genetic transformation of Nicotiana tabacum L. mesophyll protoplasts with plasmid pGreen 0029 was carried out and transient expression of reporter yfp gene in the protoplasts was observed. Using SWCNTs at the concentration of 40 μg/ml and MWCNTs at the concentration of 30 μg/ml genetic transformation of N. tabacum callus and leaf explants with nptII gene as a part of plasmid pGreen 0029 was carried out. As a result plant regeneration on selective medium containing 50 mg/lkanamycin was shown. SWCNTs-based nanocarriers de-onstrated their appli-ability to transform protoplasts as well as walled plant cells. Whereas, MWCNTs-based nano-arriers were suitable only for transformation of proto-lasts due to the limiting role of cellulose walls in cell penetration.

  14. Classification of explosives transformation products in plant tissue

    Energy Technology Data Exchange (ETDEWEB)

    Larson, S.L.; Jones, R.P. (Army Corps of Engineers, Vicksburg, MI (United States). Waterways Experiment Station); Escalon, L.; Parker, D. (AScI Corp., McLean, VA (United States))

    1999-06-01

    Explosives contamination in surface or groundwater used for the irrigation of food crops and phytoremediation of explosives-contaminated soil or water using plant-assisted biodegradation have brought about concerns as to the fate of explosives in plants. Liquid scintillation counting, high-performance liquid chromatography, and gel permeation chromatography were utilized to characterize explosives (hexahydro-1,3,5-trinitro-1,3,5-triazine and trinitrotoluene) and their metabolites in plant tissues obtained from three separate studies. Analyzing tissues of yellow nutsedge (Cyperus esculentus), corn (Zea mays), lettuce (Lacuta sativa), tomato (Lyopersicum esculentum), radish (Raphanus sativus), and parrot feather (Myriophyllum aquaticum) from three studies where exposure to explosives at nontoxic levels occurred showed that extensive transformation of the explosive contaminant occurred, variations were noted in uptake and transformation between terrestrial and aquatic plants, the products had significantly higher polarity and water solubility than the parent compounds, and the molecular sizes of the transformation products were significantly greater than those of the parent compounds.

  15. Isolation, culture, and transient transformation of plant protoplasts.

    Science.gov (United States)

    Shen, Jinbo; Fu, Jiaxin; Ma, Jin; Wang, Xiangfeng; Gao, Caiji; Zhuang, ChuXiong; Wan, Jianmin; Jiang, Liwen

    2014-06-03

    Transient gene expression in protoplasts, which has been used in several plant species, is an important and versatile tool for rapid functional gene analysis, protein subcellular localization, and biochemical manipulations. This unit describes transient gene expression by electroporation of DNA into protoplasts of Arabidopsis or tobacco suspension-cultured cells and by polyethylene glycol (PEG)-mediated DNA transformation into protoplasts derived from rice leaf sheaths. PEG-mediated DNA transformation for transient gene expression in rice protoplasts in suspension culture is also described as an alternative technique. Methods for collecting intracellular and secreted proteins are also provided.

  16. Ancillary services provided by PV power plants

    Directory of Open Access Journals (Sweden)

    Antonio PIERNO

    2016-07-01

    Full Text Available Renewable energy sources are widely utilized in distributed generation systems, and, recently, they are also considered for providing ancillary services. The paper is focused on PV plants, a survey of the most interesting papers published in the literature in the last decade is reported and the main characteristics of the technical proposals, with their advantages and limits, are evidenced. The results are schematically shown in a table that immediately gives the opportunity to be aware of what was already done, representing a reference tool.

  17. Identification of Ornamental Plant Functioned as Medicinal Plant Based on Redundant Discrete Wavelet Transformation

    Directory of Open Access Journals (Sweden)

    Kohei Arai

    2013-03-01

    Full Text Available Human has a duty to preserve the nature. One of the examples is preserving the ornamental plant. Huge economic value of plant trading, escalating esthetical value of one space and medicine efficacy that contained in a plant are some positive values from this plant. However, only few people know about its medicine efficacy. Considering the easiness to obtain and the medicine efficacy, this plant should be an initial treatment of a simple disease or option towards chemical based medicines. In order to let people get acquaint, we need a system that can proper identify this plant. Therefore, we propose to build a system based on Redundant Discrete Wavelet Transformation (RDWT through its leaf. Since its character is translation invariant that able to produce some robust features to identify ornamental plant. This system was successfully resulting 95.83% of correct classification rate.

  18. Architectural transformations in network services and distributed systems

    CERN Document Server

    Luntovskyy, Andriy

    2017-01-01

    With the given work we decided to help not only the readers but ourselves, as the professionals who actively involved in the networking branch, with understanding the trends that have developed in recent two decades in distributed systems and networks. Important architecture transformations of distributed systems have been examined. The examples of new architectural solutions are discussed. Content Periodization of service development Energy efficiency Architectural transformations in Distributed Systems Clustering and Parallel Computing, performance models Cloud Computing, RAICs, Virtualization, SDN Smart Grid, Internet of Things, Fog Computing Mobile Communication from LTE to 5G, DIDO, SAT-based systems Data Security Guaranteeing Distributed Systems Target Groups Students in EE and IT of universities and (dual) technical high schools Graduated engineers as well as teaching staff About the Authors Andriy Luntovskyy provides classes on networks, mobile communication, software technology, distributed systems, ...

  19. Transformation of legacy network management system to service oriented architecture

    Science.gov (United States)

    Sathyan, Jithesh; Shenoy, Krishnananda

    2007-09-01

    Service providers today are facing the challenge of operating and maintaining multiple networks, based on multiple technologies. Network Management System (NMS) solutions are being used to manage these networks. However the NMS is tightly coupled with Element or the Core network components. Hence there are multiple NMS solutions for heterogeneous networks. Current network management solutions are targeted at a variety of independent networks. The wide spread popularity of IP Multimedia Subsystem (IMS) is a clear indication that all of these independent networks will be integrated into a single IP-based infrastructure referred to as Next Generation Networks (NGN) in the near future. The services, network architectures and traffic pattern in NGN will dramatically differ from the current networks. The heterogeneity and complexity in NGN including concepts like Fixed Mobile Convergence will bring a number of challenges to network management. The high degree of complexity accompanying the network element technology necessitates network management systems (NMS) which can utilize this technology to provide more service interfaces while hiding the inherent complexity. As operators begin to add new networks and expand existing networks to support new technologies and products, the necessity of scalable, flexible and functionally rich NMS systems arises. Another important factor influencing NMS architecture is mergers and acquisitions among the key vendors. Ease of integration is a key impediment in the traditional hierarchical NMS architecture. These requirements trigger the need for an architectural framework that will address the NGNM (Next Generation Network Management) issues seamlessly. This paper presents a unique perspective of bringing service orientated architecture (SOA) to legacy network management systems (NMS). It advocates a staged approach in transforming a legacy NMS to SOA. The architecture at each stage is detailed along with the technical advantages and

  20. Altered phenotypes in plants transformed with chimeric tobacco peroxidase genes

    Energy Technology Data Exchange (ETDEWEB)

    Lagrimini, L.M.

    1990-12-31

    Peroxidases have been implicated in a variety of secondary metabolic reactions including lignification, cross-linking of cell wall polysaccharides, oxidation of indole-3-acetic acid, regulation of cell elongation, wound-healing, phenol oxidation, and pathogen defense. However, due to the many different isoenzymes and even more potential substrates, it has proven difficult to verify actual physiological roles for peroxidase. We are studying the molecular biology of the tobacco peroxidase genes, and have utilized genetic engineering techniques to produce transgenic plants which differ only in their expression of an individual peroxidase isoenzyme. Many of the in planta functions for any individual isoenzyme may be predicted through the morphological and physiological analysis of transformed plants.

  1. Altered phenotypes in plants transformed with chimeric tobacco peroxidase genes

    Energy Technology Data Exchange (ETDEWEB)

    Lagrimini, L.M.

    1990-01-01

    Peroxidases have been implicated in a variety of secondary metabolic reactions including lignification, cross-linking of cell wall polysaccharides, oxidation of indole-3-acetic acid, regulation of cell elongation, wound-healing, phenol oxidation, and pathogen defense. However, due to the many different isoenzymes and even more potential substrates, it has proven difficult to verify actual physiological roles for peroxidase. We are studying the molecular biology of the tobacco peroxidase genes, and have utilized genetic engineering techniques to produce transgenic plants which differ only in their expression of an individual peroxidase isoenzyme. Many of the in planta functions for any individual isoenzyme may be predicted through the morphological and physiological analysis of transformed plants.

  2. Plant functional traits predict green roof ecosystem services.

    Science.gov (United States)

    Lundholm, Jeremy; Tran, Stephanie; Gebert, Luke

    2015-02-17

    Plants make important contributions to the services provided by engineered ecosystems such as green roofs. Ecologists use plant species traits as generic predictors of geographical distribution, interactions with other species, and ecosystem functioning, but this approach has been little used to optimize engineered ecosystems. Four plant species traits (height, individual leaf area, specific leaf area, and leaf dry matter content) were evaluated as predictors of ecosystem properties and services in a modular green roof system planted with 21 species. Six indicators of ecosystem services, incorporating thermal, hydrological, water quality, and carbon sequestration functions, were predicted by the four plant traits directly or indirectly via their effects on aggregate ecosystem properties, including canopy density and albedo. Species average height and specific leaf area were the most useful traits, predicting several services via effects on canopy density or growth rate. This study demonstrates that easily measured plant traits can be used to select species to optimize green roof performance across multiple key services.

  3. Analysis of morphological traits of bird's foot trefoil plants cv. Bokor transformed with Agrobacterium rhizogenes

    Directory of Open Access Journals (Sweden)

    Nikolić Radomirka

    2005-01-01

    Full Text Available An efficient method for genetic transformation and shoot regeneration was achieved in bird's foot trefoil cv. Bokor using A. rhizogens. The transformed shoots were regenerated on hairy root segments in high frequency. After rooting and acclimation, transformed To plants were grown in experimental field. Analysis of morphological traits and chemical content in ten unintentionally chosen To bird's foot trefoil plants (genotypes no. 2 and no. 5 was performed. They were compared to those of control non-transformed plants. The traits as a number of stems per plant, length of internodes in longest stem, number of flowers per plant and plan high were very significant differed than the same traits in control plants, while there were no significant differences in the leaf area. No signs of the rol genes genotype and "T" phenotype were present. The transformed plants had significantly higher content of cellulose, while the protein and nitrogen contents of are in the range of control plants.

  4. National Plant Germplasm System: Critical Role of Customer Service

    Science.gov (United States)

    The National Plant Germplasm System (NPGS) conserves plant genetic resources, not only for use by future generations, but for immediate use by scientists and educators around the world. With a great deal of interaction between genebank curators and users of plant genetic resources, customer service...

  5. MICROBIAL TRANSFORMATIONS OF RADIONUCLIDES RELEASED FROM NUCLEAR FUEL REPROCESSING PLANTS.

    Energy Technology Data Exchange (ETDEWEB)

    FRANCIS,A.J.

    2006-10-18

    Microorganisms can affect the stability and mobility of the actinides U, Pu, Cm, Am, Np, and the fission products Tc, I, Cs, Sr, released from nuclear fuel reprocessing plants. Under appropriate conditions, microorganisms can alter the chemical speciation, solubility and sorption properties and thus could increase or decrease the concentrations of radionuclides in solution and the bioavailability. Dissolution or immobilization of radionuclides is brought about by direct enzymatic action or indirect non-enzymatic action of microorganisms. Although the physical, chemical, and geochemical processes affecting dissolution, precipitation, and mobilization of radionuclides have been investigated, we have only limited information on the effects of microbial processes. The mechanisms of microbial transformations of the major and minor actinides and the fission products under aerobic and anaerobic conditions in the presence of electron donors and acceptors are reviewed.

  6. Transformation chez les plantes fixatrices d'azote

    OpenAIRE

    Duhoux, Emile; Bogusz, Didier; Franche, Claudine

    1992-01-01

    Les techniques classiques d'amélioration végétale par hybridation sexuée, fusion somatique et mutagénèse artificielle, conduisent à l'introduction, en mélange et de manière aveugle, d'informations génétiques dans la descendance. Or, de plus en plus, on souhaite introduire seulement un ou quelques caractères nouveaux, parfaitement identifiés, sans bouleverser l'architecture génétique globale de la plante. Les transformations génétiques assurent cette opération. Il n'y a pas actuellement de mét...

  7. Emerging Roles of Agrobacterial Plant-Transforming Oncogenes in Plant Defense Reactions

    Science.gov (United States)

    Bulgakov, Victor P.; Inyushkina, Yuliya V.; Gorpenchenko, Tatiana Y.; Koren, Olga G.; Shkryl, Yuri N.; Zhuravlev, Yuri N.

    2009-01-01

    For recent years, engineering plant metabolic pathways by using rol genes looks promising in several aspects. New directions of rol-gene studies are highlighted in this work underlying the unique regulatory properties of the genes. It is known that following agrobacterial infection, the Agrobacterium rhizogenes rolA, rolB and rolC genes are transferred to plant genome, causing tumor formation and hairy root disease. In this report, we show mat these oncogenes are also involved in regulation of plant defense reactions, including the production of secondary metabolites. Situations occur where the rol genes perform their own critical function to regulate secondary metabolism by bypassing upstream plant control mechanisms and directing defense reactions via a "short cut." The rolC gene expressed in transformed plant cells is efficient in establishing an enhanced resistance of host cells to salt and temperature stresses. The emerging complexity of the rol-gene triggered effects and the involvement of signals generated by these genes in basic processes of cell biology such as calcium and ROS signaling indicate that the plant oncogenes, like some animal protooncogenes, use sophisticated strategies to affect cell growth and differentiation. The data raise the intriguing possibility that some components of plant and animal oncogene signaling pathways share common features.

  8. Transformation of pecan and regeneration of transgenic plants.

    Science.gov (United States)

    McGranahan, G H; Leslie, C A; Dandekar, A M; Uratsu, S L; Yates, I E

    1993-09-01

    A gene transfer system developed for walnut (Juglans regia L.) was successfully applied to pecan (Carya illinoensis [Wang] K. Koch). Repetitively embryogenic somatic embryos derived from open-pollinated seed of 'Elliott', 'Wichita', and 'Schley' were co-cultivated with Agrobacterium strain EHA 101/pCGN 7001, which contains marker genes for beta-glucuronidase activity and resistance to kanamycin. Several modifications of the standard walnut transformation techniques were tested, including a lower concentration of kanamycin and a modified induction medium, but these treatments had no measurable effect on efficiency of transformation. Nineteen of the 764 viable inoculated embryos produced transgenic subclones; 13 of these were from the line 'Elliott'6, 3 from 'Schley'5/3, and 3 from 'Wichita'9. Transgenic embryos of 'Wichita'9 germinated most readily and three subclones were successfully micropropagated. Three transgenic plants of one of these subclones were obtained by grafting the tissue cultured shoots to seedling pecan rootstock in the greenhouse. Gene insertion, initially detected by GUS activity, was confirmed by detection of integrated T-DNA sequences using Southern analysis.

  9. TRANSFORMATION

    Energy Technology Data Exchange (ETDEWEB)

    LACKS,S.A.

    2003-10-09

    Transformation, which alters the genetic makeup of an individual, is a concept that intrigues the human imagination. In Streptococcus pneumoniae such transformation was first demonstrated. Perhaps our fascination with genetics derived from our ancestors observing their own progeny, with its retention and assortment of parental traits, but such interest must have been accelerated after the dawn of agriculture. It was in pea plants that Gregor Mendel in the late 1800s examined inherited traits and found them to be determined by physical elements, or genes, passed from parents to progeny. In our day, the material basis of these genetic determinants was revealed to be DNA by the lowly bacteria, in particular, the pneumococcus. For this species, transformation by free DNA is a sexual process that enables cells to sport new combinations of genes and traits. Genetic transformation of the type found in S. pneumoniae occurs naturally in many species of bacteria (70), but, initially only a few other transformable species were found, namely, Haemophilus influenzae, Neisseria meningitides, Neisseria gonorrheae, and Bacillus subtilis (96). Natural transformation, which requires a set of genes evolved for the purpose, contrasts with artificial transformation, which is accomplished by shocking cells either electrically, as in electroporation, or by ionic and temperature shifts. Although such artificial treatments can introduce very small amounts of DNA into virtually any type of cell, the amounts introduced by natural transformation are a million-fold greater, and S. pneumoniae can take up as much as 10% of its cellular DNA content (40).

  10. Assessment of transformability of bacteria associated with tomato and potato plants

    NARCIS (Netherlands)

    Overbeek, van L.S.; Ray, J.L.; Elsas, van J.D.

    2007-01-01

    Transformation of plant-associated bacteria by plant DNA has never been demonstrated in agricultural fields. In total 552 bacterial isolates from stems of Ralstonia solanacearum-infected and healthy tomato plants and from stems and leaves of healthy potato plants were tested for natural genetic comp

  11. Environmental Protection Agency (EPA) Facility Registry Service (FRS) Power Plants

    Data.gov (United States)

    Department of Homeland Security — This GIS dataset contains data on wastewater treatment plants, based on EPA's Facility Registry Service (FRS) and NPDES, along with Clean Watersheds Needs Survey...

  12. EPA Facility Registry Service (FRS): Wastewater Treatment Plants

    Data.gov (United States)

    U.S. Environmental Protection Agency — This GIS dataset contains data on wastewater treatment plants, based on EPA's Facility Registry Service (FRS), EPA's Integrated Compliance Information System (ICIS)...

  13. Transformation to cloud services sourcing: required it governance capabilities

    NARCIS (Netherlands)

    Joha, A.; Janssen, M.F.W.H.A.

    2012-01-01

    The sourcing of cloud services is a relatively new type of service delivery model in which an organization gets access to IT services via a cloud service provider that is delivering services over the web to many users on a pay per use or period basis. Even though the importance of IT governance is o

  14. Transformation to cloud services sourcing: required it governance capabilities

    NARCIS (Netherlands)

    Joha, A.; Janssen, M.F.W.H.A.

    2012-01-01

    The sourcing of cloud services is a relatively new type of service delivery model in which an organization gets access to IT services via a cloud service provider that is delivering services over the web to many users on a pay per use or period basis. Even though the importance of IT governance is

  15. Soil 35S Transformation and Availability to Plants

    Institute of Scientific and Technical Information of China (English)

    ZHOUWEI; PANJIARONG

    1999-01-01

    Sulfur transformation in 3 soils maintained in a closed incubation system and its availability to plants were investigated using carrier-free 35S-SO42- and 35S-labeled ryegrass straw.For carrier-free Na235SO4 treatment,78%,70%and 64% of 35S applied were found in Ca(H2PO4)2-extractale S fraction,4%,5% and 7% in slowly soluble inorganic S,11%,15%and 18%in C-O-S,5%,7%,and 6% in C-bonded S,and 5%,7% and 6% in unidentified organic S120 days after incubation in black soil,cinnamon soil and chestnut soil,respectively.Most of 35S uptake by plants came from extractable 35SO42-,and little from C-O-35S and C-bonded 35S,In the treatment with 35S-labeled straw,51%,46%and 36% of 35S incorporated were found in Ca(H2PO4)2-extractable S fraction,7%,6% and 7% in slowly soluble inorganic S,13%,15%and 18% in C-O-S,8%,8%and 6% in C-bonded S,and 18%,25%and 35% in unidentified organic S at the end of incubation in above-mentoned three soils,respectively.Higher availability of C-O-35S,C-bonded 35S and unidentified organic 35S from 35S-labeled straw was observed in 35S-labeled straw treatment compared to carrier-free Na235SO4 treatment.

  16. EPA Facility Registry Service (FRS): Power Plants

    Data.gov (United States)

    U.S. Environmental Protection Agency — This GIS dataset contains data on power plants, based on the Energy Information Administration's EIA-860 dataset and supplemented with data from EPA's Facility...

  17. PLANT TISSUE CULTURE IN BIOTECHNOLOGY: RECENT ADVANCES IN TRANSFORMATION THROUGH SOMATIC EMBRYOGENESIS

    Directory of Open Access Journals (Sweden)

    V. A. Sidorov

    2012-08-01

    Full Text Available Plant genetic transformation has become an important biotechnology tool for the improvement of many crops. A solid foundation for the fast development and implementation of biotechnology in agriculture was provided by achievements in plant tissue culture. On the 30th anniversary of plant transformation, I report the advancements, recent challenges and shifts in methodology of transformation. The main focus of this paper will be on conventional and novel approaches for genetic improvements of soybean, cotton and corn. I will also highlight results on the transformation of these crops that have considerably been improved by modern biotechnology.

  18. Agrobacterium tumefaciens mediated transformation of the oomycete plant pathogen Phytophthora infestans

    NARCIS (Netherlands)

    Vijn, I.; Govers, F.

    2003-01-01

    Agrobacterium tumefaciens is widely used for plant DNA transformation and, more recently, has also been used to transform yeast and filamentous fungi. Here we present a protocol for Agrobacterium-mediated DNA transformation of the oomycete Phytophthora infestans, the causal agent of potato late blig

  19. Comparative Study of Feature Extraction Components from Several Wavelet Transformations for Ornamental Plants

    Directory of Open Access Journals (Sweden)

    Kohei Arai

    2014-02-01

    Full Text Available Human has a duty to preserve the nature, preserving the plant is one of the examples. This research emphasis on ornamental plant that has functionality not only as ornament plant but also as a medicinal plant. Purpose of this research is to find the best of the particular feature extraction components from several wavelet transformations. It consists of Daubechies, Dyadic, and Dual-tree complex wavelet transformation. Dyadic and Dual-tree complex wavelet transformations have shift invariant property. While Daubechies is a standard wavelet transform that widely used for many applications. This comparison is utilizing leaf image datasets from ornamental plants. From the experiments, obtained that best classification performance attained by Dual-tree complex wavelet transformation with 96.66% of overall performance result.

  20. 47 CFR 32.2001 - Telecommunications plant in service.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Telecommunications plant in service. 32.2001 Section 32.2001 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES UNIFORM SYSTEM OF ACCOUNTS FOR TELECOMMUNICATIONS COMPANIES Instructions for Balance Sheet Accounts §...

  1. Agrobacterium-mediated transformation of plants: Basic principles ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-19

    Oct 19, 2009 ... culture media composition, tissue damage, suppression and elimination of A. .... some chemicals are also some important factors that affect transformation .... mediated transformation of Vicia faba. Mol. Breed. 8: 243-254.

  2. Biological Effects of Potato Plants Transformation with Glucose Oxidase Gene and their Resistance to Hyperthermia

    Directory of Open Access Journals (Sweden)

    O.I. Grabelnych

    2017-02-01

    Full Text Available It is known that regulation of plant tolerance to adverse environmental factors is connected with short term increase of the concentration of endogenous reactive oxygen species (ROS, which are signalling molecules for the induction of protective mechanisms. Introduction and expression of heterologous gox gene, which encodes glucose oxidase enzyme in plant genome, induce constantly higher content of hydrogen peroxide in plant tissues. It is not known how the introduction of native or modified gox gene affects the plant resistance to high-temperature stress, one of the most commonly used model for the study of stress response and thermal tolerance. In this study, we investigated biological effects of transformation and evaluated the resistance to temperature stress of potato plants with altered levels of glucose oxidase expression. Transformation of potato plants by gox gene led to the more early coming out from tuber dormancy of transformed plants and slower growth rate. Transformants containing the glucose oxidase gene were more sensitive to lethal thermal shock (50 °C, 90 min than the transformant with the empty vector (pBI or untransformed plants (CK. Pre-heating of plants at 37 °C significantly weakened the damaging effect of lethal thermal shock. This attenuation was more significant in the non-transformed plants.

  3. Investigating Agrobacterium-mediated transformation of Verticillium albo-atrum on plant surfaces.

    Directory of Open Access Journals (Sweden)

    Claire J Knight

    Full Text Available BACKGROUND: Agrobacterium tumefaciens has long been known to transform plant tissue in nature as part of its infection process. This natural mechanism has been utilised over the last few decades in laboratories world wide to genetically manipulate many species of plants. More recently this technology has been successfully applied to non-plant organisms in the laboratory, including fungi, where the plant wound hormone acetosyringone, an inducer of transformation, is supplied exogenously. In the natural environment it is possible that Agrobacterium and fungi may encounter each other at plant wound sites, where acetosyringone would be present, raising the possibility of natural gene transfer from bacterium to fungus. METHODOLOGY/PRINCIPAL FINDINGS: We investigate this hypothesis through the development of experiments designed to replicate such a situation at a plant wound site. A. tumefaciens harbouring the plasmid pCAMDsRed was co-cultivated with the common plant pathogenic fungus Verticillium albo-atrum on a range of wounded plant tissues. Fungal transformants were obtained from co-cultivation on a range of plant tissue types, demonstrating that plant tissue provides sufficient vir gene inducers to allow A. tumefaciens to transform fungi in planta. CONCLUSIONS/SIGNIFICANCE: This work raises interesting questions about whether A. tumefaciens may be able to transform organisms other than plants in nature, or indeed should be considered during GM risk assessments, with further investigations required to determine whether this phenomenon has already occurred in nature.

  4. A Simplified Seed Transformation Method for Obtaining Transgenic Brassica napus Plants

    Institute of Scientific and Technical Information of China (English)

    SONG Li; ZHAO De-gang; WU Yong-jun; TIAN Xiao-e

    2009-01-01

    We report here a seed transformation of sonication-assisted,no-tissue culture to rapidly produce transgenic Brassica napus plants.This method comprises the steps of treating seeds by ultrasonic wave,inoculating Agrobacterium tumefaciens with a recombinant ChlFN-a gene and germinating directly of treatment seed on wet filter papers.The obtained transformants were verified by GUS histochemical assay and nested PCR amplification.It suggests that seed transformation has a potential use in genetic transformation of rape.

  5. Multiple host-cell recombination pathways act in Agrobacterium-mediated transformation of plant cells.

    Science.gov (United States)

    Mestiri, Imen; Norre, Frédéric; Gallego, Maria E; White, Charles I

    2014-02-01

    Using floral-dip, tumorigenesis and root callus transformation assays of both germline and somatic cells, we present here results implicating the four major non-homologous and homologous recombination pathways in Agrobacterium-mediated transformation of Arabidopsis thaliana. All four single mutant lines showed similar mild reductions in transformability, but knocking out three of four pathways severely compromised Agrobacterium-mediated transformation. Although integration of T-DNA into the plant genome is severely compromised in the absence of known DNA double-strand break repair pathways, it does still occur, suggesting the existence of other pathways involved in T-DNA integration. Our results highlight the functional redundancy of the four major plant recombination pathways in transformation, and provide an explanation for the lack of strong effects observed in previous studies on the roles of plant recombination functions in transformation.

  6. Transformational leadership in nursing service. A test of theory.

    Science.gov (United States)

    McDaniel, C; Wolf, G A

    1992-02-01

    What are the leadership dimensions that result in low turnover or work satisfaction? Intellectual stimulation, charisma, and individual consideration constitute aspects of transformational leadership that are suggested to enhance retention and staff satisfaction. In this study, the authors tested transformational leadership theory in one nursing department with an executive, 11 midlevel administrators, and 77 staff registered nurses. Findings support the predictions. Composed of teachable components, transformational factors are similar to leadership qualities described in magnet hospitals, offering positive implications for nursing administration and professional nursing practice.

  7. Critical Social Theory and Transformative Learning: Evidence in Pre-Service Teachers' Service-Learning Reflection Logs

    Science.gov (United States)

    Carrington, Suzanne; Selva, Gitta

    2010-01-01

    This paper reports on the opportunities for transformational learning experienced by a group of pre-service teachers who were engaged in service-learning as a pedagogical process with a focus on reflection. Critical social theory informed the design of the reflection process as it enabled a move away from knowledge transmission toward knowledge…

  8. Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells.

    Science.gov (United States)

    Buschmann, Henrik

    2016-01-01

    The continuing analysis of plant cell division will require additional protein localization studies. This is greatly aided by GFP-technology, but plant transformation and the maintenance of transgenic lines can present a significant technical bottleneck. In this chapter I describe a method for the Agrobacterium-mediated genetic transformation of tobacco BY-2 cells. The method allows for the microscopic analysis of fluorescence-tagged proteins in dividing cells in within 2 days after starting a coculture. This transient transformation procedure requires only standard laboratory equipment. It is hoped that this rapid method would aid researchers conducting live-cell localization studies in plant mitosis and cytokinesis.

  9. AGROBACTERIUM-MEDIATED TRANSFORMATION OF COMPOSITAE PLANTS. I. CONSTRUCTION OF TRANSGENIC PLANTS AND «HAIRY» ROOTS WITH NEW PROPERTIES

    Directory of Open Access Journals (Sweden)

    N. A.Matvieieva

    2013-02-01

    Full Text Available The review explores some of the recent advances and the author's own researchs concerning biotechnological approaches for Agrobacterium tumefaciens- and A. rhizogenes-mediated transformation of Compositae family plants. This paper reviews the results of genetic transformation of Compositae plants, including edible (Cichorium intybus, Lactuca sativa, oil (Helianthus annuus, decorative (Gerbera hybrida, medical (Bidens pilosa, Artemisia annua, Artemisia vulgaris, Calendula officinalis, Withania somnifera etc. plant species. Some Compositae genetic engineering areas are considered including creation of plants, resistant to pests, diseases and herbicides, to the effect of abiotic stress factors as well as plants with altered phenotype. The article also presents the data on the development of biotechnology for Compositae plants Cynara cardunculus, Arnica montana, Cichorium intybus, Artemisia annua "hairy" roots construction.

  10. Genome-wide profiling of genetic variation in Agrobacterium-transformed rice plants*#

    Science.gov (United States)

    Li, Wen-xu; Wu, San-ling; Liu, Yan-hua; Jin, Gu-lei; Zhao, Hai-jun; Fan, Long-jiang; Shu, Qing-yao

    2016-01-01

    Agrobacterium-mediated transformation has been widely used in producing transgenic plants, and was recently used to generate “transgene-clean” targeted genomic modifications coupled with the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas9) system. Although tremendous variation in morphological and agronomic traits, such as plant height, seed fertility, and grain size, was observed in transgenic plants, the underlying mechanisms are not yet well understood, and the types and frequency of genetic variation in transformed plants have not been fully disclosed. To reveal the genome-wide variation in transformed plants, we sequenced the genomes of five independent T0 rice plants using next-generation sequencing (NGS) techniques. Bioinformatics analyses followed by experimental validation revealed the following: (1) in addition to transfer-DNA (T-DNA) insertions, three transformed plants carried heritable plasmid backbone DNA of variable sizes (855–5216 bp) and in different configurations with the T-DNA insertions (linked or apart); (2) each transgenic plant contained an estimated 338–1774 independent genetic variations (single nucleotide variations (SNVs) or small insertion/deletions); and (3) 2–6 new Tos17 insertions were detected in each transformed plant, but no other transposable elements or bacterial genomic DNA. PMID:27921404

  11. Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants.

    Science.gov (United States)

    Majumdar, Sukanya; Garai, Saraswati; Jha, Sumita

    2011-05-01

    We have developed an efficient transformation system for Bacopa monnieri, an important Indian medicinal plant, using Agrobacterium rhizogenes strains LBA 9402 and A4. Transformed roots induced by strain LBA 9402 spontaneously dedifferentiated to callus while excised roots induced by strain A4 spontaneously showed induction of shoot buds within 10 days. PCR and RT-PCR analysis revealed the presence and expression of the rolAB and rolC genes at the transcription level in pRi A4 transformed cultures indicating that the TL-DNA was integrated retained and expressed in the A4-Ri transformed shoots. Transformed calli showed the presence of rolAB or rol A, TR and ags genes. Transformed plants showed morphological features typically seen in transgenic plants produced by A. rhizogenes. Growth and biomass accumulation was significantly higher in the transformed shoots (twofold) and roots (fourfold) than in the non-transformed (WT) plants. In pRi A4-transformed plants, the content of bacopasaponin D, bacopasaponin F, bacopaside II and bacopaside V was enhanced significantly as compared to WT plants of similar age while bacoside A3 and bacopasaponin C content was comparable with that of WT plants. Significant increase in content of five bacopa saponins could be detected in pRi 9402-transformed callus cultures. There is an overall stimulatory effect on accumulation of bacopa saponins in transformed plants and cells of B. monnieri establishing the role of endogenous elicitation by Ri T-DNA of A. rhizogenes.

  12. Using Service Oriented Architecture in a Generic Virtual Power Plant

    DEFF Research Database (Denmark)

    Andersen, Peter Bach; Poulsen, Bjarne; Træholt, Chresten;

    2009-01-01

    interesting properties that can influence the future shape of power markets. The concept holds many promises including cheaper power to the consumer, a more flexible and responsive power production and the support of a more environment-friendly development. In order to realize a software solution supporting...... the Generic Virtual Power Plant, an array of different software design principles, patterns and architectures must be applied. Especially Service Oriented Architecture (SOA) can aid in implementing the Generic Virtual Power Plant....

  13. Morphological Transformation of Plant Cells in vitro and Its Effect on Plant Growth

    Institute of Scientific and Technical Information of China (English)

    GUO Zhigang; ZENG Zhaolin; LIU Ruizhi; DENG Ying

    2005-01-01

    Enhancement of cell growth in suspension cultures is urgently needed in plant cell culture engineering. This study investigates the relationship between morphological transformation and cell growth in callus and suspension cultures of saffron cells belonging to the cell line C96 induced from Crocus sativus L. In the suspension culture, an unbalanced osmotic pressure between the intracell and extracell regions induced a large morphological transformation which affected normal division of the saffron cells. An increase in osmotic pressure caused by the addition of sucrose inhibits the vacuolation and shrinkage of cytoplasm in the cells. As the sucrose concentration increases, the total amount of accumulated biomass also increases. Besides the sucrose concentration, increased ionic strength and inoculation ratio also help restrain to a large extent the vacuolation and shrinkage of the cytoplasm in the suspended cells, which results in increased biomass. The conditions for optimal biomass are: Murashige and Skoog's (MS) medium with 40 g/L sucrose and 60% (v/v) inoculation ratio.

  14. TILLING IN THE SERVICE OF PLANT IMPROVEMENT

    Directory of Open Access Journals (Sweden)

    L. Sági

    2008-09-01

    Full Text Available A major prerequisite for description and functional characterization of the gene pool that contributes to a certain trait or constitutes the genome of a plant species is information about alleles and their combinations (haplotype among the genes involved. For identification and characterization of novel and a virtually unlimited number of alleles in a relatively high number (hundreds of genes a high-throughput method is required, which ideally also circumvents sequencing of individual alleles. The recent reverse genetics tool TILLING (‘Targeting Induced Local Lesions IN Genomes’ fulfils these criteria. Originally developed for fast identification of large numbers of alleles in a genotype saturated with induced mutations (McCallum et al. 2000, the latest improvement of TILLING, EcoTILLING (Comai et al. 2004 is especially useful for description of natural allelic variants, which represent a certain group of genotypes or even a whole species. The technique consists of several steps, which will be presented in detail. 1. Selection of primers specific for the genes to be characterized can be performed with a platform of bioinformatic softwares. The analysis includes determination of exon-intron structures, identification of genic regions with high frequency of nonsynonymous mutations, and design of best-fitting primers. 2. Mixing PCR products obtained with each selected genotype to a common standard results in the formation of heteroduplexes that contain mismatches at the position of allelic differences (point mutations, short indels and inversions, etc.. 3. Digestion of heteroduplexes by a mismatch-specific nuclease (e.g. CELI of celery produces fragments of different length, which are separated by gel electrophoresis and detected by laser excitation of IR labeled primers. Since 50-100 samples are typically analysed on one gel, high numbers of alleles can be identified in a short time. By combination of size standards and image analyis the

  15. Novel enabling technologies of gene isolation and plant transformation for improved crop protection

    Energy Technology Data Exchange (ETDEWEB)

    Torok, Tamas

    2013-02-04

    Meeting the needs of agricultural producers requires the continued development of improved transgenic crop protection products. The completed project focused on developing novel enabling technologies of gene discovery and plant transformation to facilitate the generation of such products.

  16. About the safety of power transformers in nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Petrovan Boiarciuc, M.; Muller, S.; Perigaud, G. [Research Department, SERGI Holding, 186, Avenue du General de Gaulle, 78260 Acheres (France)

    2010-07-01

    Oil-filled transformer explosions are due to electrical arcs occurring in transformer tanks. Within milliseconds, arcs vaporize the surrounding oil and the generated gas is quickly pressurized. The pressure difference between the gas bubble and the surrounding liquid oil generates one dynamic pressure peak which propagates and interacts with the tank. Then, the reflections of the pressure peak build up the static pressure, which rises and leads to the tank rupture since tanks are not designed to withstand such levels of static pressure. This results in dangerous explosions, expensive damages and possible environmental pollution. While protective walls surrounding transformers can contain the explosion and sprinklers can fight the induced fire, the current paper presents a strategy to prevent the transformer tank rupture. Once an electrical fault occurs, the fast depressurization of the tank is induced by quick oil evacuation to a reservoir in order to prevent the tank explosion. To evaluate the efficiency of this strategy, experiments and computer simulations are used. The experiments were performed on large scale transformers equipped with the protection. Besides, simulations of the consequences of an electrical arc occurring in a 200 MVA transformer geometry were run and the pressure maps obtained with and without protection were compared. (authors)

  17. Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes

    Institute of Scientific and Technical Information of China (English)

    吕德扬; 曹学远; 唐顺学; 田霞

    2000-01-01

    Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of trans-genie plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties.

  18. Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of transgenic plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties.

  19. Foliage Plant Retrieval using Polar Fourier Transform, Color Moments and Vein Features

    CERN Document Server

    Kadir, Abdul; Susanto, Adhi; Santosa, Paulus Insap

    2011-01-01

    This paper proposed a method that combines Polar Fourier Transform, color moments, and vein features to retrieve leaf images based on a leaf image. The method is very useful to help people in recognizing foliage plants. Foliage plants are plants that have various colors and unique patterns in the leaf. Therefore, the colors and its patterns are information that should be counted on in the processing of plant identification. To compare the performance of retrieving system to other result, the experiments used Flavia dataset, which is very popular in recognizing plants. The result shows that the method gave better performance than PNN, SVM, and Fourier Transform. The method was also tested using foliage plants with various colors. The accuracy was 90.80% for 50 kinds of plants.

  20. Supporting Pre-Service Teachers' Technology-Enabled Learning Design Thinking through Whole of Programme Transformation

    Science.gov (United States)

    Bower, Matt; Highfield, Kate; Furney, Pam; Mowbray, Lee

    2013-01-01

    This paper explains a development and evaluation project aimed at transforming two pre-service teacher education programmes at Macquarie University to more effectively cultivate students' technology-enabled learning design thinking. The process of transformation was based upon an explicit and sustained focus on developing university academics'…

  1. Supporting Pre-Service Teachers' Technology-Enabled Learning Design Thinking through Whole of Programme Transformation

    Science.gov (United States)

    Bower, Matt; Highfield, Kate; Furney, Pam; Mowbray, Lee

    2013-01-01

    This paper explains a development and evaluation project aimed at transforming two pre-service teacher education programmes at Macquarie University to more effectively cultivate students' technology-enabled learning design thinking. The process of transformation was based upon an explicit and sustained focus on developing university academics'…

  2. TRAINING AND EDUCATION IN THE SERVICE OF MILITARY TRANSFORMATION

    Directory of Open Access Journals (Sweden)

    Ecaterina Livia TATAR

    2012-01-01

    Full Text Available The magnitude and challenges posed by the military transformation process cannot be overcome unless the personnel involved in implementing it is well trained and motivated, for this endeavor requires a set of special skills and knowledge. Therefore, this article attempts to present two possible tools to facilitate this complex process.

  3. Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

    DEFF Research Database (Denmark)

    Trieu, A.T.; Burleigh, S.H.; Kardailsky, I.V.

    2000-01-01

    Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The se...

  4. Transformation management of primary health care services in two selected local authorities in Gauteng

    Directory of Open Access Journals (Sweden)

    W Sibaya

    2000-09-01

    Full Text Available The transformation of health services in South Africa today is governed by the political, policy and legislative frameworks. This article focuses on the transformation of a primary health care service within a local authority in Gauteng. The purpose with this article is to explore and describe the perceptions (expectations and fears of selected managers employed in this primary health care service. The results are utilised to compile a strategy (framework for transformation management and leadership within the primary health care service. A qualitative research design was utilised and the data was collected by means of individual interviews with selected managers in the service, followed by a content analysis. The expectations and fears of managers focus mainly on personnel matters, community participation/satisfaction, salaries and parity, inadequate stocks/supplies and medication, the deterioration of quality service delivery and the need for training and empowerment. These results are divided into structure, process and outcome dimensions and are embodied in the conceptual framework for the transformation and leadership strategy. It is recommended that standards for transformation management be formulated and that the quality of transformation management be evaluated accordingly.

  5. Morphological changes in atypical bird's foot trefoil plants obtained during genetic transformation by agrobacterium

    Directory of Open Access Journals (Sweden)

    Nikolić Radomirka R.

    2003-01-01

    Full Text Available Atypical plants of bird's foot trefoil (Lotus corniculatus L., Bokor cv showing altered morphological characters that deviate from a normal phenotype were found after plant regeneration from transformed tissue. It had been obtained by genetic transformation of root sections of seedlings using Agrobacterium tumefaciens vector LBA4404/pBI121 on a medium supplemented with 0.2 mg I-1 BAP. The transformants 2b arid 4a were found to have a greatly atypical habit, including shortened internodes, elongated leaves, regular leaf arrangement along the stem and thicker leaves. Inheritance of altered characters was observed in the first progeny generation, and their genetic origin was considered.

  6. Establishment of Genetic Transformation System for Miscanthus sacchariflorus and Obtaining of Its Transgenic Plants

    Institute of Scientific and Technical Information of China (English)

    Yi Zili(易自力); Zhou Puhua; Chu Chengcai; Li Xiang; Tian Wenzhong; Wang Li; Cao Shouyun; Tang Zuoshun

    2004-01-01

    The initiation and regeneration system for embryogenic callus of Miscanthus sacchariflrus is established at very high frequency. Potato proteinaseⅡ(pinⅡ) genes are introduced into the callus of Miscanthus sacchariflorus and some transgenic plants are obtained by the particle bombardment transformation system. PCR and Southern analysis show that target genes are integrated into the genome of these transgenic plants.

  7. From health services to medical markets: the commodity transformation of medical production and the nonprofit sector.

    Science.gov (United States)

    Imershein, A W; Estes, C L

    1996-01-01

    In recent years the language and logic of medical care have moved from providing medical services to marketing product lines. Analysis in this article examines this task transformation and its implications for transformation of the nonprofit sector and of the state. The authors argue that these transformations are essential explanatory elements to account for the origins of medical services in the nonprofit sector, the early exclusion of capitalist organizations from hospital care, and the changes that fostered corporate entry. To wit, medical care tasks have undergone a two-stage transformation. The first transformation changed open-ended, ill-defined services with uncertain funding into more highly organized and codified services with stable funding, attracting both capitalist enterprises and capitalist logic into the nonprofit sector. The second transformation standardized medical care tasks into product lines, a process that also challenged the status of the nonprofit organizations performing these tasks. In an analysis of the second transformation, the authors argue that this challenge is in the process of turning back upon itself, undermining the conditions that fostered capitalist entry into medical care delivery in the first place.

  8. Women Educational Leaders' Narratives: The Dynamics of Service Learning on Training and Transformation

    Science.gov (United States)

    Davis, Dannielle Joy; Major, Amanda; Cook, Debra; Bell, Janel

    2015-01-01

    Service learning strengthens all involved: Students, faculty members, the community, and higher education institutions. Benefits of service learning for students include gaining real world experiences, personal and transformative outcomes (Conway, Amel, & Gerwien's, 2009), as well as higher order thinking from reflection on the experience…

  9. Agrobacterium tumefaciens and Agrobacterium rhizogenes transformed roots of the parasitic plant Triphysaria versicolor retain parasitic competence.

    Science.gov (United States)

    Tomilov, Alexey; Tomilova, Natalya; Yoder, John I

    2007-04-01

    Parasitic plants in the Orobanchaceae invade roots of neighboring plants to rob them of water and nutrients. Triphysaria is facultative parasite that parasitizes a broad range of plant species including maize and Arabidopsis. In this paper we describe transient and stable transformation systems for Triphysaria versicolor Fischer and C. Meyer. Agrobacterium tumefaciens and Agrobacterium rhizogenes were both able to transiently express a GUS reporter in Triphysaria seedlings following vacuum infiltration. There was a correlation between the length of time seedlings were conditioned in the dark prior to infiltration and the tissue type transformed. In optimized experiments, nearly all of the vacuum infiltrated seedlings transiently expressed GUS activity in some tissue. Calluses that developed from transformed tissues were selected using non-destructive GUS staining and after several rounds of in vivo GUS selection, we recovered uniformly staining GUS calluses from which roots were subsequently induced. The presence and expression of the transgene in Triphysaria was verified using genomic PCR, RT PCR and Southern hybridizations. Transgenic roots were also obtained by inoculating A. rhizogenes into wounded Triphysaria seedlings. Stable transformed roots were identified using GUS staining or fluorescent microscopy following transformation with vectors containing GFP, dsRED or EYFP. Transgenic roots derived from both A. tumefaciens and A. rhizogenes transformations were morphologically normal and developed haustoria that attached to and invaded lettuce roots. Transgenic roots also remained competent to form haustoria in response to purified inducing factors. These transformation systems will allow an in planta assessment of genes predicted to function in plant parasitism.

  10. Influence of Phosphate on Transformation and Plant Uptake of Cadmium in Cd—amended Soils

    Institute of Scientific and Technical Information of China (English)

    XIONGLI-MING; LURU-KUN

    1991-01-01

    Phosphate was found to have neither influence on Cd transformation nor effect on plant Cd uptake in three Cd-amended upland soils.However,on submerged red earth,high phosphate dressing inhibited the transformation of Cd from exchangeable fraction to other lower-available ones.Cadmium uptake by rice plants increased simultaneously with increasing phosphate supply though plant resistance to Cd also increased at high phosphate level.Application of phosphate as an amendment for Cd-contaminated soil was therefore not recommended in view of the increasing influx of Cd into food chain especially on flooded soils.

  11. Conceptual Model Formalization in a Semantic Interoperability Service Framework: Transforming Relational Database Schemas to OWL.

    Science.gov (United States)

    Bravo, Carlos; Suarez, Carlos; González, Carolina; López, Diego; Blobel, Bernd

    2014-01-01

    Healthcare information is distributed through multiple heterogeneous and autonomous systems. Access to, and sharing of, distributed information sources are a challenging task. To contribute to meeting this challenge, this paper presents a formal, complete and semi-automatic transformation service from Relational Databases to Web Ontology Language. The proposed service makes use of an algorithm that allows to transform several data models of different domains by deploying mainly inheritance rules. The paper emphasizes the relevance of integrating the proposed approach into an ontology-based interoperability service to achieve semantic interoperability.

  12. A rapid and stable Agrobacterium-mediated transformation method of a medicinal plant Chelone glabra L.

    Science.gov (United States)

    Gao, Zhenrui; Li, Ying; Chen, Jinhua; Chen, Zhixing; Cui, Min-Long

    2015-03-01

    Transformation approach is a useful tool for the study of gene function, the mechanism of molecular regulation, and increase usefulness of components by reverse genetic approach in plants. In this study, we developed a stable and rapid method for Agrobacterium-mediated transformation of a medicinal plant Chelone glabra L. using leaf explants. Stable transformants were obtained using Agrobacterium tumefaciens strains GV2260 and GV3101 that harbored the binary vector pBI121 and contained the neomycin phosphotransferase gene (NPT II) as a selectable marker and a reporter gene β-glucuronidase (GUS). Putative transformants were identified by kanamycin selection and a histochemical assay. PCR and Southern blot analysis confirmed the integration of the GUS gene into transformed genomes as well as detected stable expression of the β-glucuronidase gene (GUS) by RT-PCR. Resulting transformed plants had morphologically normal phenotypes. This method requires two changes of medium and few leaf explants as well as the transformation efficiency of 2-8 % after 2-3 months of inoculation. This method can provide a quick and economical transformation method for reverse genetic approach to change the secondary metabolic pathway to increase useful components in C. glabra.

  13. Engineering a plant community to deliver multiple ecosystem services.

    Science.gov (United States)

    Storkey, Jonathan; Döring, Thomas; Baddeley, John; Collins, Rosemary; Roderick, Stephen; Jones, Hannah; Watson, Christine

    2015-06-01

    The sustainable delivery of multiple ecosystem services requires the management of functionally diverse biological communities. In an agricultural context, an emphasis on food production has often led to a loss of biodiversity to the detriment of other ecosystem services such as the maintenance of soil health and pest regulation. In scenarios where multiple species can be grown together, it may be possible to better balance environmental and agronomic services through the targeted selection of companion species. We used the case study of legume-based cover crops to engineer a plant community that delivered the optimal balance of six ecosystem services: early productivity, regrowth following mowing, weed suppression, support of invertebrates, soil fertility building (measured as yield of following crop), and conservation of nutrients in the soil. An experimental species pool of 12 cultivated legume species was screened for a range of functional traits and ecosystem services at five sites across a geographical gradient in the United Kingdom. All possible species combinations were then analyzed, using a process-based model of plant competition, to identify the community that delivered the best balance of services at each site. In our system, low to intermediate levels of species richness (one to four species) that exploited functional contrasts in growth habit and phenology were identified as being optimal. The optimal solution was determined largely by the number of species and functional diversity represented by the starting species pool, emphasizing the importance of the initial selection of species for the screening experiments. The approach of using relationships between functional traits and ecosystem services to design multifunctional biological communities has the potential to inform the design of agricultural systems that better balance agronomic and environmental services and meet the current objective of European agricultural policy to maintain viable food

  14. Lox-dependent gene expression in transgenic plants obtained via Agrobacterium-mediated transformation.

    Science.gov (United States)

    Shcherbak, N; Kishchenko, O; Sakhno, L; Komarnytsky, I; Kuchuk, M

    2013-01-01

    Lox sites of the Cre/lox recombination system from bacteriophage P1 were analyzed for their ability to affect on transgene expression when inserted upstream from a gene coding sequence adjacent to the right border (RB) of T-DNA. Wild and mutated types of lox sites were tested for their effect upon bar gene expression in plants obtained via Agrobacterium-mediated and biolistic transformation methods. Lox-mediated expression of bar gene, recognized by resistance of transgenic plants to PPT, occurred only in plants obtained via Agrobacterium-mediated transformation. RT-PCR analysis confirms that PPT-resistant phenotype of transgenic plants obtained via Agrobacterium-mediated transformation was caused by activation of bar gene. The plasmid with promoterless gus gene together with the lox site adjacent to the RB was constructed and transferred to Nicotiana tabacum as well. Transgenic plants exhibited GUS activity and expression of gus gene was detected in plant leaves. Expression of bar gene from the vectors containing lox site near RB allowed recovery of numerous PPT-resistant transformants of such important crops as Beta vulgaris, Brassica napus, Lactuca sativa and Solanum tuberosum. Our results demonstrate that the lox site sequence adjacent to the RB can be used to control bar gene expression in transgenic plants.

  15. Transformational leadership in the South African public service after the April 2009 national elections

    Directory of Open Access Journals (Sweden)

    Manasseh M. Mokgolo

    2012-01-01

    Full Text Available Orientation: The implementation of transformational leadership in public services after national elections has been well recorded in other parts of the world. However, this is not the case in South Africa. Research purpose: The purpose of the study is to determine whether transformational leadership has a beneficial relationship with subordinate leadership acceptance, job performance and job satisfaction.Motivation for the study: Leadership is a critical issue that the public sector needs to address in order to survive and succeed in today’s unstable environment. According to Groenewald and Ashfield (2008, transformational leadership could reduce the effects of uncertainty and change that comes with new leaders and help employees to achieve their objectives.Research design, approach and method: The sample comprised 1050 full-time employees in the public sector based in head offices. The measuring instruments included the Multifactor Leadership Questionnaire (MLQ, the Leadership Acceptance Scale (LAS, the Job Satisfaction Survey (JSS and the Job Performance Survey (JPS.Main findings: Transformational leadership had a positive correlation with subordinate leadership acceptance, performance and job satisfaction.Practical/managerial implications: Managers can train public sector leaders to be transformational leaders because of the adverse effect lack of transformation can have on employees’ attitudes in areas like satisfaction, performance and commitment.Contribution/value-add: This study makes an important contribution to our understanding of transformational leadership processes and to how the public service can improve its practices in order to render quality service to South Africans.

  16. Leading the health service into the future: transforming the NHS through transforming ourselves

    Directory of Open Access Journals (Sweden)

    Mansoor Akhtar

    2016-11-01

    Full Text Available Background: Leadership development impacts on quality of care and on workplace cultures for staff. Clinical leadership embracing transformational and other collective leadership approaches is a key enabler for developing effective workplace cultures at the micro-systems level. Following the development of a shared purpose and values framework, an internal interprofessional clinical leadership programme was set up to grow a critical community of transformational leaders across one NHS organisation in England. This programme had been unsuccessful in engaging medical doctors for more than two years. Aims and objectives: This paper shares how a dedicated, practice development-based clinical leadership programme set out to support medical doctors across one organisation with their leadership journey, equipping them to become transformational and collective leaders, and facilitators with the skills to develop and sustain person-centred, safe and effective workplace cultures. Methods: Practice development methodology, with its collaborative, inclusive and participative approach to developing person-centred cultures, combined with clinical leadership strategies, formed the basis of the programme. It emphasised the use of active and action learning, drawing on the workplace as the main resource for learning, development and improvement. Self-assessment and collective thinking about clinical leadership, together with collaborative analysis of evaluation data, led to the synthesis of insights through the use of reflection and action planning. Findings: These are presented at two levels: Five individual reflections by authors to illustrate their leadership journeys, which also demonstrate the use of a range of tools and their impact. Insights and learning include recognition of the benefits of peer support and networking, development of a disciplined approach to learning, and self-management A collaborative reflection and critique that embraced a sense of

  17. A set of modular plant transformation vectors allowing flexible insertion of up to six expression units.

    Science.gov (United States)

    Goderis, Inge J W M; De Bolle, Miguel F C; François, Isabelle E J A; Wouters, Piet F J; Broekaert, Willem F; Cammue, Bruno P A

    2002-09-01

    We have constructed a binary vector for Agrobacterium-mediated plant transformation, which has a multiple cloning site consisting of 13 hexanucleotide restriction sites, 6 octanucleotide restriction sites and 5 homing endonuclease sites. The homing endonuclease sites have the advantages to be extremely rare in natural sequences and to allow unidirectional cloning. We have also constructed a set of auxiliary vectors allowing the assembly of expression cassettes flanked by homing endonuclease sites. The expression cassettes assembled in these auxiliary vectors can be transferred into the binary vector with virtually no risk of cutting the vector within previously introduced sequences. This vector set is ideally suited for the construction of plant transformation vectors containing multiple expression cassettes and/or other elements such as matrix attachment regions. With this modular vector system, six different expression units were constructed in as many auxiliary vectors and assembled together in one plant transformation vector. The transgenic nature of Arabidopsis thaliana plants, transformed with this plant transformation vector, was assessed and the expression of each of the six genes was demonstrated.

  18. Transforming Government Service: The Importance of Dynamic Capabilities

    DEFF Research Database (Denmark)

    Pedersen, Keld

    2017-01-01

    both aiming at realizing this strategy by reengineering back office processes and implement one-stop shopping. The research contributes to the e-government literature by identifying key differences between the two cases in terms of dynamic capabilities, by identifying the importance......The Danish government has defined an ambitious e-government strategy aiming to increase both citizen centricity and the efficiency of government service production and delivery. This research uses dynamic capability theory to compare a highly successful and a less successful e-government program...

  19. Alkene Metathesis and Renewable Materials: Selective Transformations of Plant Oils

    Science.gov (United States)

    Malacea, Raluca; Dixneuf, Pierre H.

    The olefin metathesis of natural oils and fats and their derivatives is the basis of clean catalytic reactions relevant to green chemistry processes and the production of generate useful chemicals from renewable raw materials. Three variants of alkene metathesis: self-metathesis, ethenolysis and cross-metathesis applied to plant oil derivatives will show new routes to fine chemicals, bifunctional products, polymer precursours and industry intermediates.

  20. SEQUENCES OF PLANT TRANSFORMATION: THE RESULT OF THE INSERTED FOREIGN GENE EXPRESSION OR THE STRESS REACTION?

    Directory of Open Access Journals (Sweden)

    Enikeev A.G.

    2012-08-01

    Full Text Available The gene technology using in plant investigations came to the better understanding of plant physiological processes. At the same time unclear knowledge about transgenic plant physiology may occur a source of incorrect interpretation of obtained results and, consequently, wrong conclusions. In addition, the causes and mechanisms of pleiotropic effects associated with transgenic insertion and gene silencing are remaining unexplained. To solve the problem of transgenic plant physiology it is necessary to pay a close attention to physiological and biochemical peculiarities of plant-agrobacterium symbiosis, because it is a base of plant transformation. It was assumed earlier that agrobacterial transformation is a complex biotic stressing factor and transgenic plant is a long-term stressed organism. We suppose that physiological consequences of plant transformation are determined not only by foreign gene insertion, but largely by stress reaction of plant cells on agrobacterium transformation. Foreign DNA insertion to the plant recipient results in cascade of response reactions remarkably changing metabolism. The degree of such response is supposed to be in dependence on phylogenetic relations of gene donor and recipient. Cell cultures were obtained from tobacco plants (Nicotiana tabacum cv. ’Samsung’ transformed by following Agrobacterium tumefaciens strains: disarmed 669 one and LBA4400 one with hsp 101 in sense or antisense orientation. These cell cultures were used for investigations of the stress-reactions on biotic (bacterial infection agent Clavibacter michiganensis subsp. Sepedonicus and abiotic (high temperature, potassium fluoride factors. It was revealed that “sense” culture was superior to normal and “699” ones in tolerance to pointed stressing factors. Similar results were obtained for “antisense” culture, nevertheless it was a priori not expected to be tolerant. So, to assess the transformation consequence is necessary to

  1. Overexpression of several Arabidopsis histone genes increases agrobacterium-mediated transformation and transgene expression in plants.

    Science.gov (United States)

    Tenea, Gabriela N; Spantzel, Joerg; Lee, Lan-Ying; Zhu, Yanmin; Lin, Kui; Johnson, Susan J; Gelvin, Stanton B

    2009-10-01

    The Arabidopsis thaliana histone H2A-1 is important for Agrobacterium tumefaciens-mediated plant transformation. Mutation of HTA1, the gene encoding histone H2A-1, results in decreased T-DNA integration into the genome of Arabidopsis roots, whereas overexpression of HTA1 increases transformation frequency. To understand the mechanism by which HTA1 enhances transformation, we investigated the effects of overexpression of numerous Arabidopsis histones on transformation and transgene expression. Transgenic Arabidopsis containing cDNAs encoding histone H2A (HTA), histone H4 (HFO), and histone H3-11 (HTR11) displayed increased transformation susceptibility, whereas histone H2B (HTB) and most histone H3 (HTR) cDNAs did not increase transformation. A parallel increase in transient gene expression was observed when histone HTA, HFO, or HTR11 overexpression constructs were cotransfected with double- or single-stranded forms of a gusA gene into tobacco (Nicotiana tabacum) protoplasts. However, these cDNAs did not increase expression of a previously integrated transgene. We identified the N-terminal 39 amino acids of H2A-1 as sufficient to increase transient transgene expression in plants. After transfection, transgene DNA accumulates more rapidly in the presence of HTA1 than with a control construction. Our results suggest that certain histones enhance transgene expression, protect incoming transgene DNA during the initial stages of transformation, and subsequently increase the efficiency of Agrobacterium-mediated transformation.

  2. Hospitality: transformative service to children, families, and communities.

    Science.gov (United States)

    Melton, Gary B

    2014-11-01

    Hospitality is an ancient moral practice that was deeply embedded in early Judaism, Christianity, and Islam. Hospitality requires acceptance of, service to, and respect for people who lack a place in the community. The contemporary importance of this practice reflects the social disconnection and economic disadvantage of many young parents and the high frequency of separation of young people, including many young parents, from their communities. Such social deterioration substantially increases the risk of child maltreatment. Building on the proposals of the U.S. Advisory Board on Child Abuse and Neglect, Strong Communities for Children demonstrated the effectiveness of community building in reducing such risk. It further suggested the importance of both relying on and learning from hospitable people in strengthening support for children and their parents.

  3. Coming transformation in electric service: entry into cable television

    Energy Technology Data Exchange (ETDEWEB)

    Rivkin, S.R. (Rivkin and Lewis, Washington, DC); Carson, V.S.

    1982-02-04

    A combination of factors makes horizontal diversification into cable television an attractive business for electric utilities: First, if a broadband communications system were so designed, the utility could perform conservation and control functions, share facilities and personnel, and also earn revenues from the use of excess communications capacity. Second, a utility offering to build such a system would be likely to prevail over competitors for a municipal cable television franchise. Third, an investor-owned utility could anticipate minimal barriers to entry and favorable regulatory treatment of its costs and revenues from such an enterprise. The result, the authors believe, would be an evolution in electric service that will benefit both consumers and investors at a time of dramatic growth and change in the communications industry.

  4. Selectable genes for transformation of the fungal plant pathogen Glomerella cingulata f. sp. phaseoli (Colletotrichum lindemuthianum).

    Science.gov (United States)

    Rodriguez, R J; Yoder, O C

    1987-01-01

    Glomerella cingulata f. sp. phaseoli (Gcp) was transformed using either of two selectable markers: the amdS + gene of Aspergillus nidulans, which encodes acetamidase and permits growth on acetamide as the sole nitrogen source and the hygBR gene of Escherichia coli which encodes hygromycin B (Hy) phosphotransferase and permits growth in the presence of the antibiotic Hy. The amdS+ gene functioned in Gcp under control of A. nidulans regulatory signals and hygBR was expressed after fusion to a promoter from Cochliobolus heterostrophus, another filamentous ascomycete. Protoplasts to be transformed were generated with the digestive enzyme complex Novozym 234 and then were exposed to plasmid DNA in the presence of 10 mM CaCl2 and polyethylene glycol. Transformation occurred by integration of single or multiple copies of either the amdS+ or hygBR plasmid into the fungal genome. There was no evidence of autonomous plasmid replication. Transformants were mitotically stable on selective and nonselective media. However, transforming DNA in hygBR transformants was observed to occasionally rearrange during nonselective growth, resulting in fewer copies of the plasmid per genome. These transformants were capable of infecting bean (Phaseolus vulgaris), the Gcp host plant, and after recovery from infected tissue were found to have retained both the transforming DNA unrearranged in their genomes and the Hy resistance phenotype. All single-conidial cultures derived from both amdS+ and hygBR transformants had the transplanted phenotype, suggesting that transformants were homokaryons.

  5. Establishment of Plant Acceptor System for Hyperosmosis Transformation.

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker, and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic transformation system at various osmotica treatments.The results revealed that the calli induced from tomato young leaves and rice young spikelets were the ideal transfomation acceptor. The cells of calli were still vigorous when treated wity 0.75 mol/L hyperosmotic solution for 4 hours. The differentiation rates of calli varied from 7.5% to 93.3% in different media. The bud differentiation was apparently inhibited by hyperosmotic treatments. O.75mol/L sucrose hypertonic solution and O.2mol/L CaCl2 solution were favorable hyperosmoticum and hypoosmoticum respectively.

  6. A novel approach to the generation of seamless constructs for plant transformation

    DEFF Research Database (Denmark)

    Kronbak, Remy; Ingvardsen, Christina R.; Madsen, Claus K.;

    2014-01-01

    Background: When creating plant transformation vectors, full control of nucleotides flanking the insert in the final construct may be desirable. Modern ligase-independent methods for DNA-recombination are based on linearization by classical type II restriction endonucleases (REs) alone or in comb......Background: When creating plant transformation vectors, full control of nucleotides flanking the insert in the final construct may be desirable. Modern ligase-independent methods for DNA-recombination are based on linearization by classical type II restriction endonucleases (REs) alone...... on wheat and barley endosperm cells for transient gfp expression.Conclusions: All nucleotides flanking an insert in a biolistic plant transformation vector can be customized by means of SRL in combination with SLIC. Especially type IIS REs promote an efficient cloning result. Based on our findings, we...

  7. Scheduling and location issues in transforming service fleet vehicles to electric vehicles

    DEFF Research Database (Denmark)

    Mirchandani, Pitu; Madsen, Oli B.G.; Adler, Jonathan

    There is much reason to believe that fleets of service vehicles of many organizations will transform their vehicles that utilize alternative fuels which are more sustainable. The electric vehicle is a good candidate for this transformation, especially which “refuels” by exchanging its spent...... batteries with charged ones. This paper discusses the issues that must be addressed if a transit service were to use electric vehicles, principally the issues related to the limited driving range of each electric vehicle’s set of charged batteries and the possible detouring for battery exchanges...

  8. Where Does the Transformation of Precipitated Ceria Nanoparticles in Hydroponic Plants Take Place?

    Science.gov (United States)

    Ma, Yuhui; Zhang, Peng; Zhang, Zhiyong; He, Xiao; Zhang, Junzhe; Ding, Yayun; Zhang, Jing; Zheng, Lirong; Guo, Zhi; Zhang, Lijuan; Chai, Zhifang; Zhao, Yuliang

    2015-09-01

    Cerium oxide nanoparticles (CeO2 NPs) have been found to be partly biotransformed from Ce(IV) to Ce(III) in plants, yet the transformation process and mechanism are not fully understood. Here, we try to clarify the specific site and necessary conditions for the transformation of precipitated CeO2 NPs in hydroponic cucumber plants. Three different treatment modes were adopted according to whether the NPs were incubated with roots all the time or not. Results showed that exposure modes significantly affect the translocation and transformation of CeO2 NPs. In the normal exposure mode, Ce was present as a Ce(IV) and Ce(III) mixture in the roots and shoots, and the proportion of Ce(III) in the shoots was enhanced obviously with the increase of exposure time. The results of short-time incubation and petiole exposure modes suggested that CeO2 NPs could not be reduced within a short incubation time (3 h) or be further reduced inside the plant tissues. It was deduced that root surfaces are the sites, and the physicochemical interaction between the NPs and root exudates at the nanobio interface is the necessary condition for the transformation of CeO2 NPs in plant systems. These results will contribute to understanding the transformation mechanism of CeO2 and other metal-based NPs and properly evaluate their ecological effects.

  9. Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation.

    Science.gov (United States)

    Shikata, Masahito; Ezura, Hiroshi

    2016-01-01

    Tomato is a model plant for fruit development, a unique feature that classical model plants such as Arabidopsis and rice do not have. The tomato genome was sequenced in 2012 and tomato is becoming very popular as an alternative system for plant research. Among many varieties of tomato, Micro-Tom has been recognized as a model cultivar for tomato research because it shares some key advantages with Arabidopsis including its small size, short life cycle, and capacity to grow under fluorescent lights at a high density. Mutants and transgenic plants are essential materials for functional genomics research, and therefore, the availability of mutant resources and methods for genetic transformation are key tools to facilitate tomato research. Here, we introduce the Micro-Tom mutant database "TOMATOMA" and an efficient transformation protocol for Micro-Tom.

  10. Genetic transformation of the plant pathogens Phytophthora capsici and Phytophthora parasitica.

    Science.gov (United States)

    Bailey, A M; Mena, G L; Herrera-Estrella, L

    1991-08-11

    Phytophthora capsici and P.parasitica were transformed to hygromycin B resistance using plasmids pCM54 and pHL1, which contain the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the Ustilago maydis heat shock hsp70 gene. Enzymes Driselase and Novozyme 234 were used to generate protoplasts which were then transformed following exposure to plasmid DNA and polyethylene glycol 6000. Transformation frequencies of over 500 transformants per micrograms of DNA per 1 x 10(6) protoplasts were obtained. Plasmid pCM54 appears to be transmitted in Phytophthora spp. as an extra-chromosomal element through replication, as shown by Southern blot hybridization and by the loss of plasmid methylation. In addition, transformed strains retained their capacity of infecting Serrano pepper seedlings and Mc. Intosh apple fruits, the host plants for P.capsici and P.parasitica, respectively.

  11. Basics of RTCM 3.1 Transformation Messages Standard for GNSS Positioning Services

    OpenAIRE

    Eleiche, M

    2009-01-01

    As concerns the use of RTCM observation correction data in GNSS positioning services, the coordinates are resulting within the ITRF or in a regional ITRF-realization (e.g. ETRF89 in Europe) as geocentric (x,y,z) or ellipsoidal (ϕ,λ ,h) coordinates. The users of GNSS services must however normally present their results in the coordinates of regional or local horizontal and vertical datum systems. Therefore, coordinate transformations are necessary. Currently, the pre-calculated tra...

  12. Experimental investigations of overvoltages in 6kV station service cable networks of thermal power plants

    Energy Technology Data Exchange (ETDEWEB)

    Vukelja, P.I.; Naumov, R.M.; Drobnjak, G.V.; Mrvic, J.D. [Nikola Tesla Inst., Belgrade (Yugoslavia)

    1996-12-31

    The paper presents the results of experimental investigations of overvoltages on 6kV isolated neutral station service cable networks of thermal power plants. The overvoltages were recorded with capacitive voltage measurement systems made at the Nikola Tesla Institute. Wideband capacitive voltage measurement systems recorded a flat response from below power frequencies to 10MHz. Investigations of overvoltages were performed for appearance and interruption of metal earth faults, intermittent earth faults, switching operation of HV motors switchgear, switching operation of transformers switchgear, and transfer of the network supply from one transformer to another. On the basis of these investigations, certain measures are proposed for limiting overvoltages and for the reliability of station service of thermal power plants.

  13. 78 FR 1825 - Notice of Establishment of an Animal and Plant Health Inspection Service Stakeholder Registry

    Science.gov (United States)

    2013-01-09

    ... Inspection Service Stakeholder Registry AGENCY: Animal and Plant Health Inspection Service, USDA. ACTION... Service stakeholder registry. FOR FURTHER INFORMATION CONTACT: Ms. Hallie Zimmers, Advisor for State and Stakeholder Relations, Legislative and Public Affairs, APHIS, room 1147, 1400 Independence Avenue...

  14. Production of Transgenic Tall Fescue Plants with Enhanced Stress Tolerances by Agrobacterium tumefaciens-Mediated Transformation

    Institute of Scientific and Technical Information of China (English)

    WU Guan-ting; CHEN Jin-qing; HU Zhang-hua; LANG Chun-xiu; CHEN Xiao-yun; WANG Fu-lin; JIN Wei; XIA Ying-wu

    2006-01-01

    In order to improve stress tolerances of turf-type tall fescue (Festuca arundinacea Schreb.), Agrobacterium tumefaciens strain EHA105 carrying plasmid pCMD containing stress tolerance-related CBF1 gene from Arabidopsis thaliana was used to transform mature seeds-derived embryogenic calli of four cultivars. A total of 112 transgenic plants were regenerated from 32 independent lines and verified by histochemical detection of GUS activity, PCR assay and Southern hybridization analysis. The transformation frequency ranged from 0.92 to 2.87% with apparent differences among the cultivars. Stress tolerances of transgenic plants were enhanced, which was shown by the facts that transgenic plants had distinct growth superiority and significantly higher survival rate than non-transformed ones under high salinity and high osmosis stresses,and that relative electronic conductivity of in vitro leaves treated with low and high temperatures, dehydration and high salinity stresses was 25-30% lower in transgenic plants than in control plants. In addition, it was observed that growth of transgenic plants was inhibited due to constitutive overexpression of CBF1 gene under normal environmental conditions.

  15. Bacterial communities involved in sulfur transformations in wastewater treatment plants.

    Science.gov (United States)

    Meyer, Daniel Derrossi; de Andrade, Pedro Avelino Maia; Durrer, Ademir; Andreote, Fernando Dini; Corção, Gertrudes; Brandelli, Adriano

    2016-12-01

    The main sulfate-reducing (SRB) and sulfur-oxidizing bacteria (SOB) in six wastewater treatment plants (WWTPs) located at southern Brazil were described based on high-throughput sequencing of the 16S rDNA. Specific taxa of SRB and SOB were correlated with some abiotic factors, such as the source of the wastewater, oxygen content, sample type, and physical chemical attributes of these WWTPs. When the 22 families of SRB and SOB were clustered together, the samples presented a striking distribution, demonstrating grouping patterns according to the sample type. For SOB, the most abundant families were Spirochaetaceae, Chromatiaceae, Helicobacteriaceae, Rhodospirillaceae, and Neisseriaceae, whereas, for SRB, were Syntrophaceae, Desulfobacteraceae, Nitrospiraceae, and Desulfovibriaceae. The structure and composition of the major families related to the sulfur cycle were also influenced by six chemical attributes (sulfur, potassium, zinc, manganese, phosphorus, and nitrogen). Sulfur was the chemical attribute that most influenced the variation of bacterial communities in the WWTPs (λ = 0.14, p = 0.008). The OTUs affiliated to Syntrophus showed the highest response to the increase of total sulfur. All these findings can contribute to improve the understanding in relation to the sulfur-oxidizing and sulfate-reducing communities in WWTPs aiming to reduce H2S emissions.

  16. From PCK to TPACK: Developing a Transformative Model for Pre-Service Science Teachers

    Science.gov (United States)

    Jang, Syh-Jong; Chen, Kuan-Chung

    2010-01-01

    New science teachers should be equipped with the ability to integrate and design the curriculum and technology for innovative teaching. How to integrate technology into pre-service science teachers' pedagogical content knowledge is the important issue. This study examined the impact on a transformative model of integrating technology and peer…

  17. Integration and transformation of rural service delivery: The role of management information and decision support systems

    CSIR Research Space (South Africa)

    Mashiri, M

    2005-01-01

    Full Text Available The paper deals with two main themes: 1) the integration and transformation of rural service delivery; and 2) role of management information and decision support systems in this process. Referring specifically to the types of rural areas, conditions...

  18. A decision Enhancement Service for Stakeholder Analysis to Achieve Transformations in the Public Sector

    NARCIS (Netherlands)

    Knol, Arjan; Janssen, Marijn; Sol, H G

    2015-01-01

    Sourcing has become a popular practice for public sector managers aiming for transformations to save costs and improve service delivery. Nevertheless, public sector sourcing often fails due to stakeholder resistance and power struggles, stressing the need for stakeholder analysis. This paper present

  19. Lock hopper values for coal gasification plant service

    Science.gov (United States)

    Schoeneweis, E. F.

    1977-01-01

    Although the operating principle of the lock hopper system is extremely simple, valve applications involving this service for coal gasification plants are likewise extremely difficult. The difficulties center on the requirement of handling highly erosive pulverized coal or char (either in dry or slurry form) combined with the requirement of providing tight sealing against high-pressure (possibly very hot) gas. Operating pressures and temperatures in these applications typically range up to 1600 psi (110bar) and 600F (316C), with certain process requirements going even higher. In addition, and of primary concern, is the need for reliable operation over long service periods with the provision for practical and economical maintenance. Currently available data indicate the requirement for something in the order of 20,000 to 30,000 open-close cycles per year and a desire to operate at least that long without valve failure.

  20. Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

    DEFF Research Database (Denmark)

    Christiansen, S.K.; Knudsen, S.; Giese, H.

    1995-01-01

    Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces, GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta...

  1. Plant metabolites of the Siberian flora. Chemical transformations and the scope of practical application

    Energy Technology Data Exchange (ETDEWEB)

    Shults, Elvira E; Raldugin, Victor A; Volcho, Konstantin P; Salakhutdinov, Nariman F; Tolstikov, Genrikh A [N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk (Russian Federation)

    2007-07-31

    The results of studies of some terpenoids, alkaloids and phenolic derivatives isolated from Siberian plants are summarised. The structures of the compounds studied are presented and the chemical transformations of the available terpenoids and alkaloids are considered. Examples of practical application of natural compounds and their derivatives are given.

  2. Plant metabolites of the Siberian flora. Chemical transformations and the scope of practical application

    Science.gov (United States)

    Shults, Elvira E.; Raldugin, Victor A.; Volcho, Konstantin P.; Salakhutdinov, Nariman F.; Tolstikov, Genrikh A.

    2007-07-01

    The results of studies of some terpenoids, alkaloids and phenolic derivatives isolated from Siberian plants are summarised. The structures of the compounds studied are presented and the chemical transformations of the available terpenoids and alkaloids are considered. Examples of practical application of natural compounds and their derivatives are given.

  3. Community-Centered Service Learning: A Transformative Lens for Teaching-Learning in Nursing.

    Science.gov (United States)

    Yancey, Nan Russell

    2016-04-01

    Although service learning has been proposed as a teaching-learning modality in response to an ongoing challenge to transform nursing education, there is a risk to community and student when service learning is poorly conceived. A community-centered service learning approach founded on a nursing theoretical perspective and community model is explored as a way to honor the wisdom and perspective of the community in changing while illuminating a new way of being a nurse in community for the nursing student. © The Author(s) 2016.

  4. Effect of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity and Other Factors in Successful Plant Transformation

    Directory of Open Access Journals (Sweden)

    Sandip S. Magdum

    2013-08-01

    Full Text Available Agrobacterium tumefaciens infection and antibiotic wash are the critical steps of Agrobacterium mediated plant transformation procedure, most time responsible for lower transformation efficiency due to necrosis and phytotoxicity caused by biotic stress of Agrobacterium and abiotic stress by antibiotics respectively. Ammi majus Egyptian origin medicinal plant and Pearl millet cereal grain crop were studied for their stress responses to Agrobacterium mediated transformation (AMT. Agrobacterium strains LBA4404 (O.D.=0.6-0.8 and EHA105 (O.D.=0.2-0.4 were used for transformation experiments to infect calli of Ammi majus and embryogenic calli of Pearl millet respectively. Incase of antibiotic wash, Cefotaxime 500 mg L-1 was used for LBA4404 infected Ammi majus calli and Timentin 300 mg L-1 was used for EHA105 infected embryogenic calli of Pearl millet. Effects of Agrobacterium infection, antibiotic and NaOCl washes on Agrobacterium removal and both explants physiological changes during transformation experimental procedures were studied. At the end of the experiments explants survival efficiency of Ammi majus and pearl millet were 8% and 5% respectively. Biotic and abiotic stress factors responsible for lower efficiency were investigated with various other factors and strategies were discussed which are need to be considered for higher transformation events and target tissue survival.

  5. The new rtcm 3. 1 transformation messages declaration, generation from reference transformations and implementation as a server-client-concept for GNss services

    OpenAIRE

    Jäger, Reiner; Kälber, Simone

    2008-01-01

    As concerns the use of RTCM observation correction data in GNSS positioning services, the coordinates are resulting within the ITRF or in a regional ITRF-realization (e.g. ETRF89) as geocentric (x,y,z) or ellipsoidal (B,L,h) coordinates. The users of GNSS services must however normally present their results in the coordinates of regional or local horizontal and vertical datum systems. Therefore, coordinate transformations are necessary. Currently, the pre-calculated transformation parameters ...

  6. Agrobacterium rhizogenes-mediated transformation of the parasitic plant Phtheirospermum japonicum.

    Directory of Open Access Journals (Sweden)

    Juliane K Ishida

    Full Text Available BACKGROUND: Plants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: We developed transient and stable transformation systems for Phtheirospermum japonicum, a facultative parasitic plant in the Orobanchaceae. The transformation protocol was established by a combination of sonication and acetosyringone treatments using the hairy-root-inducing bacterium, Agrobacterium rhizogenes and young seedlings. Transgenic hairy roots of P. japonicum were obtained from cotyledons 2 to 3 weeks after A. rhizogenes inoculation. The presence and the expression of transgenes in P. japonicum were verified by genomic PCR, Southern blot and RT-PCR methods. Transgenic roots derived from A. rhizogenes-mediated transformation were able to develop haustoria on rice and maize roots. Transgenic roots also formed apparently competent haustoria in response to 2,6-dimethoxy-1,4-benzoquinone (DMBQ, a haustorium-inducing chemical. Using this system, we introduced a reporter gene with a Cyclin B1 promoter into P. japonicum, and visualized cell division during haustorium formation. CONCLUSIONS: We provide an easy and efficient method for hairy-root transformation of P. japonicum. Transgenic marker analysis revealed that cell divisions during haustorium development occur 24 h after DMBQ treatment. The protocols described here will allow functional analysis of genes involved in plant parasitism.

  7. 9 CFR 113.6 - Animal and Plant Health Inspection Service testing.

    Science.gov (United States)

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Animal and Plant Health Inspection Service testing. 113.6 Section 113.6 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION... STANDARD REQUIREMENTS Applicability § 113.6 Animal and Plant Health Inspection Service testing. A...

  8. Development of an Agrobacterium-mediated stable transformation method for the sensitive plant Mimosa pudica.

    Science.gov (United States)

    Mano, Hiroaki; Fujii, Tomomi; Sumikawa, Naomi; Hiwatashi, Yuji; Hasebe, Mitsuyasu

    2014-01-01

    The sensitive plant Mimosa pudica has long attracted the interest of researchers due to its spectacular leaf movements in response to touch or other external stimuli. Although various aspects of this seismonastic movement have been elucidated by histological, physiological, biochemical, and behavioral approaches, the lack of reverse genetic tools has hampered the investigation of molecular mechanisms involved in these processes. To overcome this obstacle, we developed an efficient genetic transformation method for M. pudica mediated by Agrobacterium tumefaciens (Agrobacterium). We found that the cotyledonary node explant is suitable for Agrobacterium-mediated transformation because of its high frequency of shoot formation, which was most efficiently induced on medium containing 0.5 µg/ml of a synthetic cytokinin, 6-benzylaminopurine (BAP). Transformation efficiency of cotyledonary node cells was improved from almost 0 to 30.8 positive signals arising from the intron-sGFP reporter gene by using Agrobacterium carrying a super-binary vector pSB111 and stabilizing the pH of the co-cultivation medium with 2-(N-morpholino)ethanesulfonic acid (MES) buffer. Furthermore, treatment of the explants with the detergent Silwet L-77 prior to co-cultivation led to a two-fold increase in the number of transformed shoot buds. Rooting of the regenerated shoots was efficiently induced by cultivation on irrigated vermiculite. The entire procedure for generating transgenic plants achieved a transformation frequency of 18.8%, which is comparable to frequencies obtained for other recalcitrant legumes, such as soybean (Glycine max) and pea (Pisum sativum). The transgene was stably integrated into the host genome and was inherited across generations, without affecting the seismonastic or nyctinastic movements of the plants. This transformation method thus provides an effective genetic tool for studying genes involved in M. pudica movements.

  9. Development of an Agrobacterium-mediated stable transformation method for the sensitive plant Mimosa pudica.

    Directory of Open Access Journals (Sweden)

    Hiroaki Mano

    Full Text Available The sensitive plant Mimosa pudica has long attracted the interest of researchers due to its spectacular leaf movements in response to touch or other external stimuli. Although various aspects of this seismonastic movement have been elucidated by histological, physiological, biochemical, and behavioral approaches, the lack of reverse genetic tools has hampered the investigation of molecular mechanisms involved in these processes. To overcome this obstacle, we developed an efficient genetic transformation method for M. pudica mediated by Agrobacterium tumefaciens (Agrobacterium. We found that the cotyledonary node explant is suitable for Agrobacterium-mediated transformation because of its high frequency of shoot formation, which was most efficiently induced on medium containing 0.5 µg/ml of a synthetic cytokinin, 6-benzylaminopurine (BAP. Transformation efficiency of cotyledonary node cells was improved from almost 0 to 30.8 positive signals arising from the intron-sGFP reporter gene by using Agrobacterium carrying a super-binary vector pSB111 and stabilizing the pH of the co-cultivation medium with 2-(N-morpholinoethanesulfonic acid (MES buffer. Furthermore, treatment of the explants with the detergent Silwet L-77 prior to co-cultivation led to a two-fold increase in the number of transformed shoot buds. Rooting of the regenerated shoots was efficiently induced by cultivation on irrigated vermiculite. The entire procedure for generating transgenic plants achieved a transformation frequency of 18.8%, which is comparable to frequencies obtained for other recalcitrant legumes, such as soybean (Glycine max and pea (Pisum sativum. The transgene was stably integrated into the host genome and was inherited across generations, without affecting the seismonastic or nyctinastic movements of the plants. This transformation method thus provides an effective genetic tool for studying genes involved in M. pudica movements.

  10. INFLUENCE OF BIOLOGICAL AND THERMAL TRANSFORMED SEWAGE SLUDGE APPLICATION ON MANGANESE CONTENT IN PLANTS AND SOIL

    Directory of Open Access Journals (Sweden)

    Małgorzata Koncewicz-Baran

    2014-10-01

    Full Text Available A great variety of sewage sludge treatment methods, due to the agent (chemical, biological, thermal leads to the formation of varying ‘products’ properties, including the content of heavy metals forms. The aim of the study was to determine the effects of biologically and thermally transformed sewage sludge on the manganese content in plants and form of this element in the soil. The study was based on a two-year pot experiment. In this study was used stabilized sewage sludge collected from Wastewater Treatment Plant Krakow – ”Płaszów” and its mixtures with wheat straw in the gravimetric ratio 1:1 in conversion to material dry matter, transformed biologically (composting by 117 days in a bioreactor and thermally (in the furnace chamber with no air access by the following procedure exposed to temperatures of 130 °C for 40 min → 200 °C for 30 min. In both years of the study biologically and thermally transformed mixtures of sewage sludge with wheat straw demonstrated similar impact on the amount of biomass plants to the pig manure. Bigger amounts of manganese were assessed in oat biomass than in spring rape biomass. The applied sewage sludge and its biologically and thermally converted mixtures did not significantly affect manganese content in plant biomass in comparison with the farmyard manure. The applied fertilization did not modify the values of translocation and bioaccumulation ratios of manganese in the above-ground parts and roots of spring rape and oat. No increase in the content of the available to plants forms of manganese in the soil after applying biologically and thermally transformed sewage sludge mixtures with straw was detected. In the second year, lower contents of these manganese forms were noted in the soil of all objects compared with the first year of the experiment.

  11. Sensoria Patterns: Augmenting Service Engineering with Formal Analysis, Transformation and Dynamicity

    Science.gov (United States)

    Wirsing, Martin; Hölzl, Matthias; Acciai, Lucia; Banti, Federico; Clark, Allan; Fantechi, Alessandro; Gilmore, Stephen; Gnesi, Stefania; Gönczy, László; Koch, Nora; Lapadula, Alessandro; Mayer, Philip; Mazzanti, Franco; Pugliese, Rosario; Schroeder, Andreas; Tiezzi, Francesco; Tribastone, Mirco; Varró, Dániel

    The IST-FET Integrated Project Sensoria is developing a novel comprehensive approach to the engineering of service-oriented software systems where foundational theories, techniques and methods are fully integrated into pragmatic software engineering processes. The techniques and tools of Sensoria encompass the whole software development cycle, from business and architectural design, to quantitative and qualitative analysis of system properties, and to transformation and code generation. The Sensoria approach takes also into account reconfiguration of service-oriented architectures (SOAs) and re-engineering of legacy systems.

  12. Diagnostics of the power oil-filled transformer equipment of thermal power plants

    Science.gov (United States)

    Eltyshev, D. K.; Khoroshev, N. I.

    2016-08-01

    Problems concerning improvement of the diagnostics efficiency of the electrical facilities and functioning of the generation and distribution systems through the examples of the power oil-filled transformers, as the responsible elements referring to the electrical part of thermal power plants (TPP), were considered. Research activity is based on the fuzzy logic system allowing working both with statistical and expert information presented in the form of knowledge accumulated during operation of the power oil-filled transformer facilities. The diagnostic algorithm for various types of transformers, with the use of the intellectual estimation model of its thermal state on the basis of the key diagnostic parameters and fuzzy inference hierarchy, was developed. Criteria for taking measures allowing preventing emergencies in the electric power systems were developed. The fuzzy hierarchical model for the state assessment of the power oil-filled transformers of 110 kV, possessing high degree of credibility and setting quite strict requirements to the limits of variables of the equipment diagnostic parameters, was developed. The most frequent defects of the transformer standard elements, related with the disturbance of the isolation properties and instrumentation operation, were revealed after model testing on the real object. Presented results may be used both for the express diagnostics of the transformers state without disconnection from the power line and for more detailed analysis of the defects causes on the basis of the advanced list of the diagnostic parameters; information on those parameters may be received only after complete or partial disconnection.

  13. Transforming your service: the right evidence at the right time and place.

    Science.gov (United States)

    Sutton, Anthea

    2016-12-01

    This virtual issue is published to coincide with the CILIP Health Libraries Group Conference 2016. The theme of the conference is Knowledge for Healthcare (KFH), the development framework for health care library and knowledge services (LKS), published by Health Education England. Transforming the service, and delivering the right evidence at the right time and place, is a key strategic focus of KFH. Therefore, this virtual issue draws on content published in Health Information and Libraries Journal (HILJ) in the last 2 years, which demonstrate initiatives in health care LKS in line with the transformation strands outlined in KFH. The virtual issue follows the same structure as a regular issue of HILJ, containing one review article, a series of original articles and our three regular features: Dissertations into Practice, Teaching and Learning in Action and International Perspectives and Initiatives. © 2016 Health Libraries Group.

  14. Selenium Content, Influential Factors Within the Plant and the Transformation of Different Selenium Specification

    Directory of Open Access Journals (Sweden)

    LIU Yuan-yuan

    2014-12-01

    Full Text Available The paper collected relevant literatures on selenium and explored the function to plant, selenium content, influential factors and selenium specification and transformation. We believed that there should be more deep researches on function of selenium to plant. Approaches of molecular, genetic engineering and isotope could be employed to breed selenium rich crops and possibilities in practice. More efforts should be spent on the technologies research for improving selenium level in crops under natural soil conditions to sustainably utilize the selenium resources.

  15. Transformation

    DEFF Research Database (Denmark)

    Peters, Terri

    2011-01-01

    Artiklen diskuterer ordet "transformation" med udgangspunkt i dels hvorledes ordet bruges i arkitektfaglig terminologi og dels med fokus på ordets potentielle indhold og egnethed i samme teminologi.......Artiklen diskuterer ordet "transformation" med udgangspunkt i dels hvorledes ordet bruges i arkitektfaglig terminologi og dels med fokus på ordets potentielle indhold og egnethed i samme teminologi....

  16. Transformation

    DEFF Research Database (Denmark)

    Peters, Terri

    2011-01-01

    Artiklen diskuterer ordet "transformation" med udgangspunkt i dels hvorledes ordet bruges i arkitektfaglig terminologi og dels med fokus på ordets potentielle indhold og egnethed i samme teminologi.......Artiklen diskuterer ordet "transformation" med udgangspunkt i dels hvorledes ordet bruges i arkitektfaglig terminologi og dels med fokus på ordets potentielle indhold og egnethed i samme teminologi....

  17. Arizona Public Service - Alternative Fuel (Hydrogen) Pilot Plant Design Report

    Energy Technology Data Exchange (ETDEWEB)

    James E. Francfort

    2003-12-01

    Hydrogen has promise to be the fuel of the future. Its use as a chemical reagent and as a rocket propellant has grown to over eight million metric tons per year in the United States. Although use of hydrogen is abundant, it has not been used extensively as a transportation fuel. To assess the viability of hydrogen as a transportation fuel and the viability of producing hydrogen using off-peak electric energy, Pinnacle West Capital Corporation (PNW) and its electric utility subsidiary, Arizona Public Service (APS) designed, constructed, and operates a hydrogen and compressed natural gas fueling station—the APS Alternative Fuel Pilot Plant. This report summarizes the design of the APS Alternative Fuel Pilot Plant and presents lessons learned from its design and construction. Electric Transportation Applications prepared this report under contract to the U.S. Department of Energy’s Advanced Vehicle Testing Activity. The Idaho National Engineering and Environmental Laboratory manages these activities for the Advanced Vehicle Testing Activity.

  18. Nitrogen transformations and retention in planted and artificially aerated constructed wetlands.

    Science.gov (United States)

    Maltais-Landry, Gabriel; Maranger, Roxane; Brisson, Jacques; Chazarenc, Florent

    2009-02-01

    Nitrogen (N) processing in constructed wetlands (CWs) is often variable, and the contribution to N loss and retention by various pathways (nitrification/denitrification, plant uptake and sediment storage) remains unclear. We studied the seasonal variation of the effects of artificial aeration and three different macrophyte species (Phragmites australis, Typha angustifolia and Phalaris arundinacea) on N processing (removal rates, transformations and export) using experimental CW mesocosms. Removal of total nitrogen (TN) was higher in summer and in planted and aerated units, with the highest mean removal in units planted with T. angustifolia. Export of ammonium (NH(4)(+)), a proxy for nitrification limitation, was higher in winter, and in unplanted and non-aerated units. Planted and aerated units had the highest export of oxidized nitrogen (NO(y)), a proxy for reduced denitrification. Redox potential, evapotranspiration (ETP) rates and hydraulic retention times (HRT) were all predictors of TN, NH(4)(+) and NO(y) export, and significantly affected by plants. Denitrification was the main N sink in most treatments accounting for 47-62% of TN removal, while sediment storage was dominant in unplanted non-aerated units and units planted with P. arundinacea. Plant uptake accounted for less than 20% of the removal. Uncertainties about the long-term fate of the N stored in sediments suggest that the fraction attributed to denitrification losses could be underestimated in this study.

  19. An intellectual property sharing initiative in agricultural biotechnology: development of broadly accessible technologies for plant transformation.

    Science.gov (United States)

    Chi-Ham, Cecilia L; Boettiger, Sara; Figueroa-Balderas, Rosa; Bird, Sara; Geoola, Josef N; Zamora, Pablo; Alandete-Saez, Monica; Bennett, Alan B

    2012-06-01

    The Public Intellectual Property Resource for Agriculture (PIPRA) was founded in 2004 by the Rockefeller Foundation in response to concerns that public investments in agricultural biotechnology benefiting developing countries were facing delays, high transaction costs and lack of access to important technologies due to intellectual property right (IPR) issues. From its inception, PIPRA has worked broadly to support a wide range of research in the public sector, in specialty and minor acreage crops as well as crops important to food security in developing countries. In this paper, we review PIPRA's work, discussing the failures, successes, and lessons learned during its years of operation. To address public sector's limited freedom-to-operate, or legal access to third-party rights, in the area of plant transformation, we describe PIPRA's patent 'pool' approach to develop open-access technologies for plant transformation which consolidate patent and tangible property rights in marker-free vector systems. The plant transformation system has been licensed and deployed for both commercial and humanitarian applications in the United States (US) and Africa, respectively.

  20. pORE: a modular binary vector series suited for both monocot and dicot plant transformation.

    Science.gov (United States)

    Coutu, Catherine; Brandle, James; Brown, Dan; Brown, Kirk; Miki, Brian; Simmonds, John; Hegedus, Dwayne D

    2007-12-01

    We present a series of 14 binary vectors suitable for Agrobacterium-mediated transformation of dicotyledonous plants and adaptable for biolistic transformation of monocotyledonous plants. The vector size has been minimized by eliminating all non-essential elements from the vector backbone and T-DNA regions while maintaining the ability to replicate independently. The smallest of the vector series is 6.3 kb and possesses an extensive multiple cloning site with 21 unique restriction endonuclease sites that are compatible with common cloning, protein expression, yeast two-hybrid and other binary vectors. The T-DNA region was engineered using a synthetic designer oligonucleotide resulting in an entirely modular system whereby any vector element can be independently exchanged. The high copy number ColE1 origin of replication has been included to enhance plasmid yield in Escherichia coli. FRT recombination sites flank the selectable marker cassette regions and allow for in planta excision by FLP recombinase. The pORE series consists of three basic types; an 'open' set for general plant transformation, a 'reporter' set for promoter analysis and an 'expression' set for constitutive expression of transgenes. The sets comprise various combinations of promoters (P (HPL), P (ENTCUP2) and P (TAPADH)), selectable markers (nptII and pat) and reporter genes (gusA and smgfp).

  1. Teaching caring and competence: Student transformation during an older adult focused service-learning course.

    Science.gov (United States)

    Brown, Karen M; Bright, Leslie M

    2017-08-18

    Innovative teaching strategies develop nurses' knowledge, skills, and attitudes while simultaneously integrating the art of caring and transforming attitudes toward adults over age 65. The study's purpose was to explore students' experiences and attitudes toward older adults with cognitive and/or physical limitations as well as the effects on students' knowledge and skills during a baccalaureate nursing, course which included a service-learning experience. Service-learning synthesizes meaningful community service, academic instruction, and reflection. Participants included baccalaureate students enrolled in a service-learning nursing course focused on older adults. This retrospective, qualitative, phenomenological study used reflective journals and an online survey to explore baccalaureate nursing students' experiences toward older adults with cognitive and/or physical limitations. Themes included initial attitudes of anticipation, apprehension, anxiety, and ageist stereotypes. Final attitudes included a "completely changed perspective" of caring, compassion, and respect indicative of a rewarding, "life-changing" experience. Participants cited enhanced learning, especially in the areas of patient-centered care, collaboration, communication, advocacy, empathy, assessment skills, and evidence-based practice. This innovative teaching strategy led to transformed attitudes toward older adults, reduced fear of older adult populations, an increased desire to work with older adults, and the ability to form a transpersonal, caring relationship while enhancing nursing knowledge and skills. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. TRANSFORMER

    Science.gov (United States)

    Baker, W.R.

    1959-08-25

    Transformers of a type adapted for use with extreme high power vacuum tubes where current requirements may be of the order of 2,000 to 200,000 amperes are described. The transformer casing has the form of a re-entrant section being extended through an opening in one end of the cylinder to form a coaxial terminal arrangement. A toroidal multi-turn primary winding is disposed within the casing in coaxial relationship therein. In a second embodiment, means are provided for forming the casing as a multi-turn secondary. The transformer is characterized by minimized resistance heating, minimized external magnetic flux, and an economical construction.

  3. The dsdA gene from Escherichia coli provides a novel selectable marker for plant transformation.

    Science.gov (United States)

    Erikson, Oskar; Hertzberg, Magnus; Näsholm, Torgny

    2005-02-01

    Plants are sensitive to D-serine, but functional expression of the dsdA gene, encoding D-serine ammonia lyase, from Escherichia coli can alleviate this toxicity. Plants, in contrast to many other organisms, lack the common pathway for oxidative deamination of D-amino acids. This difference in metabolism has major consequences for plant responses to D-amino acids, since several D-amino acids are toxic to plants even at relatively low concentrations. Therefore, introducing an enzyme specific for a phytotoxic D-amino acid should generate a selectable characteristic that can be screened. Here we present the use of the dsdA gene as a selectable marker for transformation of Arabidopsis. D-serine ammonia lyase catalyses the deamination of D-serine into pyruvate, water and ammonium. dsdA transgenic seedlings can be clearly distinguished from wild type, having an unambiguous phenotype immediately following germination when selected on D-serine containing medium. The dsdA marker allows flexibility in application of the selective agent: it can be applied in sterile plates, in foliar sprays or in liquid culture. Selection with D-serine resistance was compared with selection based on kanamycin resistance, and was found to generate similar transformation frequencies but also to be more unambiguous, more rapid and more versatile with respect to the way the selective agent can be supplied.

  4. Metabolic engineering by plastid transformation as a strategy to modulate isoprenoid yield in plants.

    Science.gov (United States)

    Hasunuma, Tomohisa; Kondo, Akihiko; Miyake, Chikahiro

    2010-01-01

    Plants synthesize a large number of isoprenoid compounds that have diverse structures and functions. All isoprenoids are synthesized through consecutive condensation of five-carbon precursors, isopentenyl diphosphate (IPP) and its allyl isomer dimethylallyl diphosphate (DMAPP). With recent success in the cloning of genes that encode the enzymes of isoprenoid biosynthesis, genetic engineering strategies for the improvement of plant isoprenoid metabolism have emerged. Plastid transformation technology offers attractive features in plant genetic engineering. It has many advantages over nuclear genome transformation: high-level foreign protein expression, no need for a transit peptide, absence of gene silencing, and convenient transgene stacking in operons. We demonstrated that this technology is a remarkable tool for the production of isoprenoids in plants through metabolic engineering. The expression of bacterial genes encoding CrtW (beta-carotene ketolase) and CrtZ (beta-carotene hydroxylase) or cyanobacterial genes encoding DXR (1-deoxy-D-xylulose-5-phosphate reductoisomerase) in the plastid genome leads to alteration in isoprenoid content of tobacco leaves.

  5. Reproduction of Meloidogyne javanica on Plant Roots Genetically Transformed by Agrobacterium rhizogenes.

    Science.gov (United States)

    Verdejo, S; Jaffee, B A; Mankau, R

    1988-10-01

    Reproduction of Meloidogyne javanica was compared on several Agrobacterium rhizogenes-transformed root cultures under monoxenic conditions. M. javanica reproduced on all transformed roots tested; however, more females and eggs were obtained on potato and South Australian Early Dwarf Red tomato than on bindweed, Tropic tomato, lima bean, or carrot. Roots that grew at moderate rates into the agar and produced many secondary roots supported the highest reproduction. Numbers of females produced in cultures of transformed potato roots increased with increasing nematode inoculum levels, whether inoculum was dispersed eggs or juveniles. Females appeared smaller, produced fewer eggs, and were found in coalesced galls at the higher inoculum levels. The ratio between the final and initial population decreased sharply as the juvenile inoculum increased. The second-stage juvenile was preferred to dispersed eggs or egg masses for inoculation of tissue culture systems because quantity and viability of inoculum were easily assessed. Meloidogyne javanica reared on transformed root cultures were able to complete their life cycles on new transformed root cultures or greenhouse tomato plants.

  6. A Multilevel Approach for Social Transformations and its Implications on Service Design Education

    DEFF Research Database (Denmark)

    Morelli, Nicola; Götzen, Amalia De

    2017-01-01

    Service design education is still young and it is still interpreted in many different ways, according to the design programs where it usually plays a minor role. Very few education schemes provide a complete curriculum on service design, facing the challenge of equipping a designer who will want...... to have a dominant role in providing solutions for people, supporting new forms of social innovation that happen through new processes both in public and private services. This paper tries to map the new value-creation process in a three level structure, proposing a framework of new competences and tools...... that are being developed in design education and research. The value in use, the infrastructuring and the governance levels are explained with the specific tools and approaches required according to the specific aim of the design activity: that is, wether it is about understanding, transforming or communicating...

  7. Aging Management Guideline for commercial nuclear power plants: Power and distribution transformers

    Energy Technology Data Exchange (ETDEWEB)

    Toman, G.; Gazdzinski, R. [Sandia National Labs., Albuquerque, NM (United States)

    1994-05-01

    This Aging Management Guideline (AMG) provides recommended methods for effective detection and mitigation of age-related degradation mechanisms in power and distribution transformers important to license renewal in commercial nuclear power plants. The intent of this AMG to assist plant maintenance and operations personnel in maximizing the safe, useful life of these components. It also supports the documentation of effective aging management programs required under the License Renewal Rule 10 CFR Part 54. This AMG is presented in a manner which allows personnel responsible for performance analysis and maintenance to compare their plant-specific aging mechanisms (expected or already experienced) and aging management program activities to the more generic results and recommendations presented herein.

  8. Arsenic transformations in the soil-rhizosphere-plant system: fundamentals and potential application to phytoremediation.

    Science.gov (United States)

    Fitz, Walter J; Wenzel, Walter W

    2002-11-13

    This paper reviews major processes that potentially affect the fate of arsenic in the rhizosphere of plants. Rhizosphere interactions are deemed to play a key role in controlling bioavailability to crop plants and for a better understanding and improvement of phytoremediation technologies. Substantial progress has been made towards an understanding of As transformation processes in soils. However, virtually no information is available that directly addresses the fate of As in the rhizosphere. We are proposing a conceptual model of the fate of As in the soil-rhizosphere-plant system by integrating the state-of-the art knowledge available in the contributing disciplines. Using this model and recent studies on hyperaccumulation of As, we discuss research needs and the potential application of rhizosphere processes to the development of phytoremediation technologies for As-polluted soils.

  9. Transformation of japonica rice with RHL gene and salt tolerance of the transgenic rice plant

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Overexpression of the yeast HAL2 gene increases salt tolerance of yeast and plant. Rice HAL2-like (RHL) gene was introduced into a japonica rice cultivar HJ19 with Agrobacterium tumefaciens-mediated transformation. Transgenic plants in R0 generation were selected on the principle of GUS-positive, RHL gene PCR-positive and normal growth. Hygromycin-resistant plants of some transgenic lines in R1 generation increased salt tolerance during the seedling and booting stage, being less damaged in the cytomembrane and stronger in leaf tissue viability under salt stress during booting period. Southern analysis of transgenic lines tolerant to salt in R1 generation showed that the RHL gene expression cassette had been successfully integrated into rice genome. Moreover, gene engineering breeding methodology and really salt-tolerant rice cultivar were discussed.

  10. Estimating the number of integrations in transformed plants by quantitative real-time PCR

    Directory of Open Access Journals (Sweden)

    Vaira Anna Maria

    2002-10-01

    Full Text Available Abstract Background When generating transformed plants, a first step in their characterization is to obtain, for each new line, an estimate of how many copies of the transgene have been integrated in the plant genome because this can deeply influence the level of transgene expression and the ease of stabilizing expression in following generations. This task is normally achieved by Southern analysis, a procedure that requires relatively large amounts of plant material and is both costly and labour-intensive. Moreover, in the presence of rearranged copies the estimates are not correct. New approaches to the problem could be of great help for plant biotechnologists. Results By using a quantitative real-time PCR method that requires limited preliminary optimisation steps, we achieved statistically significant estimates of 1, 2 and 3 copies of a transgene in the primary transformants. Furthermore, by estimating the copy number of both the gene of interest and the selectable marker gene, we show that rearrangements of the T-DNA are not the exception, and probably happen more often than usually recognised. Conclusions We have developed a rapid and reliable method to estimate the number of integrated copies following genetic transformation. Unlike other similar procedures, this method is not dependent on identical amplification efficiency between the PCR systems used and does not need preliminary information on a calibrator. Its flexibility makes it appropriate in those situations where an accurate optimisation of all reaction components is impossible or impractical. Finally, the quality of the information produced is higher than what can be obtained by Southern blot analysis.

  11. Domestic work count! Characteristics and transformations of the domestic service in Spain

    Directory of Open Access Journals (Sweden)

    Raquel Martínez Buján

    2015-03-01

    Full Text Available This paper explores the characteristics of and transformations that Spanish domestic service has undergone in the since 2000. The paper also analyzes the domestic service’s contribution to the Spanish economy since the beginning of the country’s economic crisis. Relying on quantitative analysis of the domestic work data from the Active Population Survey, the most relevant changes identified are the increase of its demand and the consolidation of migrants workers in it. The paper also looks at the tasks carried out in the households of employers. The recent conversion of the domestic services in a nonprofessional care sector seems to mitigate the burden of personal care work that Spanish families aim to provide to their older relatives. This transformation of domestic work into care work has changed some traditional features of this occupation: the outcomes of qualitative data show changes in the intensification of the tasks carried out, a tendency to higher male workers, a reappearance of live-in workers, and key modifications in the relationship between employer families and employees. Finally, this paper incorporates a short assessment of Real Royal Decree 1620/2011 (which regulates domestic service and Law 39/2006 (Law on the Promotion of Personal Autonomy and Care for Dependent Persons to analyze which is the legal space delegate to the labor category related with the non-professional caregivers in private homes.

  12. [Study on transformation of snowdrop lectin gene to chrysanthemum and aphid resistance of the transgenic plants].

    Science.gov (United States)

    Wang, Guan-Lin; Liu, Yan-Hong; Guo, Shao-Hua; Wang, Yu; Ji, Yan; Fang, Hong-Jun

    2004-12-01

    Agrobacterium-mediated transformation in chrysanthemum was studied to prevent the insect pest of aphid (Mizus persicae). The gna gene was successfully transferred into chrysanthemum by leaf dish, and 93 transgenic clones were obtained. The highest transformation frequency 11.21% was achieved on the optimization facts, which were medium YEB with pH5.6, bacterial concentration OD600 = 0.4, precultivation for one day, cocultivation for four days, the cocultivation media supplemented with GA3 0.5 mg/L and leaf explants growed for 45 days. The results from PCR and FQ-PCR analysis confirmed that gna gene was integrated into the genome of chrysanthemum plants. The insect bioassay with aphid showed that the aphid resistance of different transgenic plants was difference, and the rate of aphid population inhibition of them were from 10% to 84% with an average rate of 39.4%. The leaf-extracts from different transgenic plants showed varying actinties in red-blood cell bioassay.

  13. Handling Arabidopsis plants: growth, preservation of seeds, transformation, and genetic crosses.

    Science.gov (United States)

    Rivero, Luz; Scholl, Randy; Holomuzki, Nicholas; Crist, Deborah; Grotewold, Erich; Brkljacic, Jelena

    2014-01-01

    Growing healthy plants is essential for the advancement of Arabidopsis thaliana (Arabidopsis) research. Over the last 20 years, the Arabidopsis Biological Resource Center (ABRC) has collected and developed a series of best-practice protocols, some of which are presented in this chapter. Arabidopsis can be grown in a variety of locations, growth media, and environmental conditions. Most laboratory accessions and their mutant or transgenic derivatives flower after 4-5 weeks and set seeds after 7-8 weeks, under standard growth conditions (soil, long day, 23 ºC). Some mutant genotypes, natural accessions, and Arabidopsis relatives require strict control of growth conditions best provided by growth rooms, chambers, or incubators. Other lines can be grown in less-controlled greenhouse settings. Although the majority of lines can be grown in soil, certain experimental purposes require utilization of sterile solid or liquid growth media. These include the selection of primary transformants, identification of homozygous lethal individuals in a segregating population, or bulking of a large amount of plant material. The importance of controlling, observing, and recording growth conditions is emphasized and appropriate equipment required to perform monitoring of these conditions is listed. Proper conditions for seed harvesting and preservation, as well as seed quality control, are also described. Plant transformation and genetic crosses, two of the methods that revolutionized Arabidopsis genetics, are introduced as well.

  14. Transformation of maize embryogenic calli mediated by ultrasonication and regeneration of fertile transgenic plants

    Institute of Scientific and Technical Information of China (English)

    张宏; 王国英; 谢友菊; 戴景瑞; 许宁; 赵南明; 李太源; 田颖川; 乔利亚; 莽克强

    1997-01-01

    An insecticidal protein gene isolated from Bacillus thuringiensis was transferred into maize by using ultrasonication. The fertile transgenic plants and their progeny were obtained. The Southern hybridization results indi-cated that the foreign gene had integrated into the maize genome. It has been found that the acoustic intensity and the duration of treatment are the important parameters influencing transformation efficiency by ultrasonication. The maxi-mum relative transformation frequency of 34. 1 % was achieved after 30 min of sonication at 0. 5 W/cm2 acoustic inten-sity. With appropriate parameters the ultrasonication ran make a number of micropores formed on the cell surface and minimize the treatment damage to the foreign DNA molecules, thus facilitating the DNA molecules to enter the cells.

  15. Strategies for transforming human service organizations into learning organizations: knowledge management and the transfer of learning.

    Science.gov (United States)

    Austin, Michael J

    2008-01-01

    This analysis describes the nature of a learning organization, defines the boundaries of evidence-informed practice, identifies the elements of knowledge management, and specifies the elements of the transfer of learning. A set of principles are presented to guide managers in transforming human service organizations into learning organizations along with a set of implementation strategies that can inform participants of the values and benefits of knowledge management. This analysis features concepts and principles adapted and synthesized from research in diverse fields, such as evidence-based health care and the for-profit sector related to learning organizations, knowledge management, and the transfer of learning.

  16. Antioxidant potential of Agrobacterium-transformed and non-transformed Physalis ixocarpa plants grown in vitro and ex vitro 

    Directory of Open Access Journals (Sweden)

    Katarzyna Bergier

    2012-12-01

    Full Text Available Introduction:Oxidative stress is involved in pathogenesis of a number of chronic diseases hence is an increasing interest in plant-derived natural antioxidants with respect to their potential health benefits. Plants from the genus Physalis are particularly rich in secondary metabolites and show significant antioxidant potential. Recent development in transgenic research has opened new possibilities for enhanced production of secondary metabolites with plant cell and organ cultures. The hairy root-regenerated Physalis ixocarpa plants grown in vitro and ex vitro were compared to the non-transformed plants with respect to their antioxidant potential.Material/Methods:The total antioxidant capacity (TAC, the contents of total phenols and ascorbate were evaluated in fruits, flowers, leaves and roots of P. ixocarpa using the ferric reducing antioxidant power assay (FRAP, the Folin-Ciocalteu method and the 2,2’-dipyridyl method, respectively.Results/Discussion:The antioxidant profiles, in terms of TAC, ascorbate and phenols were organ-specific and depended on the culture conditions. Neither the total phenol content nor the ascorbate level appeared to determine the TAC of the studied plant extracts. The aqueous extracts exhibited lower antioxidant activities than the acetone ones indicating that lipophilic antioxidants made a major contribution to TAC of the plant tissues. Agrobacterium rhizogenes-mediated transformation changed the antioxidant status with respect to TAC, phenols and ascorbate and this effect was observed in the plants grown in vitro and ex vitro. 

  17. A review of impacts by invasive exotic plants on forest ecosystem services

    Science.gov (United States)

    Kevin Devine; Songlin. Fei

    2011-01-01

    Many of our forest ecosystems are at risk due to the invasion of exotic invasive plant species. Invasive plant species pose numerous threats to ecosystems by decreasing biodiversity, deteriorating ecosystem processes, and degrading ecosystem services. Literature on Kentucky's most invasive exotic plant species was examined to understand their potential impacts on...

  18. Testing a measure of organizational learning capacity and readiness for transformational change in human services.

    Science.gov (United States)

    Bess, Kimberly D; Perkins, Douglas D; McCown, Diana L

    2011-01-01

    Transformative organizational change requires organizational learning capacity, which we define in terms of (1) internal and (2) external organizational systems alignment, and promoting a culture of learning, including (3) an emphasis on exploration and information, (4) open communication, (5) staff empowerment, and (6) support for professional development. We shortened and adapted Watkins and Marsick's Dimensions of Learning Organizations Questionnaire into a new 16-item Organizational Learning Capacity Scale (OLCS) geared more toward nonprofit organizations. The OLCS and its subscales measuring each of the above 6 dimensions are unusually reliable for their brevity. ANOVAs for the OLCS and subscales clearly and consistently confirmed extensive participant observations and other qualitative data from four nonprofit human service organizations and one local human service funding organization.

  19. Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene

    Institute of Scientific and Technical Information of China (English)

    金松恒; 蒋德安; 李雪芹; 孙骏威

    2004-01-01

    Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased sig-nificantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types,although they showed 70% lower rate of photosynthesis, whichRubsico activase and/or the reduction in carbamylation.was likely an acclimation response to the reduction in Rubsico activase and/or the reduction in carbamylation.

  20. Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene

    Institute of Scientific and Technical Information of China (English)

    金松恒; 蒋德安; 李雪芹; 孙骏威

    2004-01-01

    Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased significantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types,although they showed 70% lower rate of photosynthesis, which was likely an acclimation response to the reduction inRubsico activase and/or the reduction in carbamylation.

  1. Effect of Weak Light on the Photoassimilates Distribution and Transformation of Young Grape Plants

    Institute of Scientific and Technical Information of China (English)

    ZHAN Ji-cheng; HUANG Wei-dong; WANG Li-jun

    2002-01-01

    The effect of weak light on the photoassimilates distribution and transformation of young Jingyu grape plants ( Vitis vinefera L. cv. Jingyn) were studied by shading. Compared to the grape grown under natural light intensity environment, the net photosynthetic rate diurnal variation curve of experimental plants grown in weak light intensity environment was remarkably lower. Its leaf and stem biomass ratio increased when light intensity decreased, the root and new shoot biomass ratio showed an opposite trend. The 14C-photoassimilates was mostly distributed to young leaves and stem, a little was distributed to root. The metabolism of 14C-photoassimilates distributed to the entire grape body were also changed under weak light intensity environment.

  2. Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene.

    Science.gov (United States)

    Jin, Song-Heng; Jiang, De-An; Li, Xue-Qin; Sun, Jun-Wei

    2004-08-01

    Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased significantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types, although they showed 70% lower rate of photosynthesis, which was likely an acclimation response to the reduction in Rubsico activase and/or the reduction in carbamylation.

  3. Evaluation of Nicotiana tabacum plants transformed for the expression of verocytotoxic Escherichia coli antigens.

    Directory of Open Access Journals (Sweden)

    Angela Lombardi

    2015-07-01

    Full Text Available Two transgenic Nicotiana tabacum plants, carrying respectively the F18 adhesive fimbriae and the B subunit of verocytotoxin genes from O138 Verocytotoxic E.coli serotype were developed as a model of edible vaccine. Tobacco plants were transformed by agroinfection according to Rossi et al. (2013 stably.  The F18 adhesive fimbriae and VT2e B-subunit were placed under control of the GLOB promoter for the seed-specific protein expression. Agrobacterium tumefaciens binary vector system is an efficient tool to transform plant cells; however, the exogenous gene integrates at semi-random into the nuclear chromosome. PCR products, using specific oligonucleotides putatively encoding the B-subunit of VT2e-B and F18 fimbriae were identified on agarose gel (1.5% - 0.9% as bands with a length of 270 and 519 base pairs, respectively. We showed that the foreign VT2e-B and F18 fimbriae genes were stably integrated into the tobacco genome. Northern blot and Western blot analyses carried out respectively on total mRNA and total soluble protein extract obtained from seeds. For each line, the obtained amount of antigens is sufficient for subsequent oral immunization trials. Three lines of tobacco seeds (F18, VT2e-B, and WT were seeded in homogeneous conditions and were harvested simultaneously. Tobacco plants were analysed also by optical and electronic microscope in different phases of growth. Germination of transgenic seeds were delayed of three/five days compared to WT in two replicated experiments, suggesting that genetic manipulation may influenced mechanisms leading to germination. In conclusion the genes coding for VT2e-B and the F18 are stably maintained in the seeds and obtained tobacco seeds represent a valid strategy to ferry antigenic proteins to the gut and a promising non-invasive method of vaccination in pig industry.

  4. Leveraging Terminology Services for Extract-Transform-Load Processes: A User-Centered Approach.

    Science.gov (United States)

    Peterson, Kevin J; Jiang, Guoqian; Brue, Scott M; Liu, Hongfang

    2016-01-01

    Terminology services serve an important role supporting clinical and research applications, and underpin a diverse set of processes and use cases. Through standardization efforts, terminology service-to-system interactions can leverage well-defined interfaces and predictable integration patterns. Often, however, users interact more directly with terminologies, and no such blueprints are available for describing terminology service-to-user interactions. In this work, we explore the main architecture principles necessary to build a user-centered terminology system, using an Extract-Transform-Load process as our primary usage scenario. To analyze our architecture, we present a prototype implementation based on the Common Terminology Services 2 (CTS2) standard using the Patient-Centered Network of Learning Health Systems (LHSNet) project as a concrete use case. We perform a preliminary evaluation of our prototype architecture using three architectural quality attributes: interoperability, adaptability and usability. We find that a design-time focus on user needs, cognitive models, and existing patterns is essential to maximize system utility.

  5. 7 CFR 58.122 - Approved plants under USDA inspection and grading service.

    Science.gov (United States)

    2010-01-01

    ...) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) REGULATIONS AND STANDARDS UNDER THE AGRICULTURAL MARKETING ACT OF 1946 AND THE EGG PRODUCTS... and Grading Service 1 Purpose § 58.122 Approved plants under USDA inspection and grading service....

  6. Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment.

    Science.gov (United States)

    Gao, Caixia; Long, Danfeng; Lenk, Ingo; Nielsen, Klaus Kristian

    2008-10-01

    Agrobacterium-mediated transformation and particle bombardment are the two most widely used methods for genetically modifying grasses. Here, these two systems are compared for transformation efficiency, transgene integration and transgene expression when used to transform tall fescue (Festuca arundinacea Schreb.). The bar gene was used as a selectable marker and selection during tissue culture was performed using 2 mg/l bialaphos in both callus induction and regeneration media. Average transformation efficiency across the four callus lines used in the experiments was 10.5% for Agrobacterium-mediated transformation and 11.5% for particle bombardment. Similar transgene integration patterns and co-integration frequencies of bar and uidA were observed in both gene transfer systems. However, while GUS activity was detected in leaves of 53% of the Agrobacterium transformed lines, only 20% of the bombarded lines showed GUS activity. Thus, Agrobacterium-mediated transformation appears to be the preferred method for producing transgenic tall fescue plants.

  7. Targeted Mutagenesis in Plant Cells through Transformation of Sequence-Specific Nuclease mRNA.

    Directory of Open Access Journals (Sweden)

    Thomas J Stoddard

    Full Text Available Plant genome engineering using sequence-specific nucleases (SSNs promises to advance basic and applied plant research by enabling precise modification of endogenous genes. Whereas DNA is an effective means for delivering SSNs, DNA can integrate randomly into the plant genome, leading to unintentional gene inactivation. Further, prolonged expression of SSNs from DNA constructs can lead to the accumulation of off-target mutations. Here, we tested a new approach for SSN delivery to plant cells, namely transformation of messenger RNA (mRNA encoding TAL effector nucleases (TALENs. mRNA delivery of a TALEN pair targeting the Nicotiana benthamiana ALS gene resulted in mutation frequencies of approximately 6% in comparison to DNA delivery, which resulted in mutation frequencies of 70.5%. mRNA delivery resulted in three-fold fewer insertions, and 76% were 10bp. In an effort to increase mutation frequencies using mRNA, we fused several different 5' and 3' untranslated regions (UTRs from Arabidopsis thaliana genes to the TALEN coding sequence. UTRs from an A. thaliana adenine nucleotide α hydrolases-like gene (At1G09740 enhanced mutation frequencies approximately two-fold, relative to a no-UTR control. These results indicate that mRNA can be used as a delivery vehicle for SSNs, and that manipulation of mRNA UTRs can influence efficiencies of genome editing.

  8. Transformative Learning of Pre-Service Teachers during Study Abroad in Reggio Emilia, Italy: A Case Study

    Science.gov (United States)

    Vatalaro, Angela; Szente, Judit; Levin, Judith

    2015-01-01

    The present paper explores the transformative learning of five pre-service teachers participating in a two-week study abroad program to Reggio Emilia, Italy. The study was conducted in order to understand how a study abroad program could contribute to pre-service teachers' content knowledge, teaching practices, and global competence. Through a…

  9. Construction of a Plant Transformation-ready Expression cDNA Library for Thellungiella halophila Using Recombination Cloning

    Institute of Scientific and Technical Information of China (English)

    Wan-Song Ni; Zhi-Yong Lei; Xi Chen; David J. Oliver; Cheng-Bin Xiang

    2007-01-01

    Salt cress (Thellungiella halophila), a close relative of the model plant Arabidopsis thaliana L., is an extremophile that is adapted to harsh saline environments. To mine salt-tolerance genes from this species, we constructed an entry cDNA library from the salt cress plant treated with salt-stress by using a modified cDNA synthesis and an improved recombinationassisted cDNA library construction method that is completely free of manipulations involving restriction enzymes and DNA ligase. This cDNA library construction procedure is significantly simplified and the quality of the cDNA library is improved. This entry cDNA library was subsequently shuttled into the destination binary vector pCB406 designed for plant transformation and expression via recombination-assisted cloning. The library is plant transformation ready and is used to transform Arabidopsis on a large scale in order to create a large collection of transgenic lines for functional gene mining.

  10. Can plant oncogenes inhibit programmed cell death? The rolB oncogene reduces apoptosis-like symptoms in transformed plant cells.

    Science.gov (United States)

    Gorpenchenko, Tatiana Y; Aminin, Dmitry L; Vereshchagina, Yuliya V; Shkryl, Yuri N; Veremeichik, Galina N; Tchernoded, Galina K; Bulgakov, Victor P

    2012-09-01

    The rolB oncogene was previously identified as an important player in ROS metabolism in transformed plant cells. Numerous reports indicate a crucial role for animal oncogenes in apoptotic cell death. Whether plant oncogenes such as rolB can induce programmed cell death (PCD) in transformed plant cells is of particular importance. In this investigation, we used a single-cell assay based on confocal microscopy and fluorescent dyes capable of discriminating between apoptotic and necrotic cells. Our results indicate that the expression of rolB in plant cells was sufficient to decrease the proportion of apoptotic cells in steady-state conditions and diminish the rate of apoptotic cells during induced PCD. These data suggest that plant oncogenes, like animal oncogenes, may be involved in the processes mediating PCD.

  11. Organogenesis and plant selection by kanamycin of strawberry in vitro aiming at genetic transformation

    Directory of Open Access Journals (Sweden)

    Letícia Reis

    2015-08-01

    Full Text Available The success of genetic transformation of the strawberry (Fragaria x ananassa is strongly influenced by the cultivar and depends on efficient regeneration and plant-transformed selection. In plant regeneration, efficient organogenesis depends on the concentrations of plant growth regulators that are present in the culture medium, and the response varies according to the explant type and cultivar. Similarly, in the selection of transformed plants, the sensitivity to selective agents induced by marker genes depends on the concentration of the selective substance in the regeneration medium and must be adjusted according to the chosen cultivar. The aim of this work was to test different combinations and concentrations of TDZ (Thidiazuron and IBA (indolebutyric-acid added to the base medium MS (MURASHIGE; SKOOG, 1962 for shoot regeneration of Camiño Real and Festival cultivars from leaf and petiole explants. In the case of ‘Camiño Real’, 1.0, 1.5 and 2.0 mg L-1 of TDZ combined with 0.1, 0.3 and 0.5 mg L-1 of IBA were tested. In the case of ‘Festival’, 1.0, 1.5 and 2.0 mg L-1 of TDZ combined with 0.1 and 0.3 mg L-1 of IBA were evaluated. Another aim was to define the lowest concentration of the selective agent Kanamycin (marker gene npt II able to inhibit regeneration of the untransformed shoots. For this purpose, Kanamycin doses of 0, 2, 4, 6, 8 and 10 mg L-1 were added to the pre-established regeneration medium. In the petiole explant test, normal shoot regeneration did not occur; thus, only shoot regeneration and the sensitivity to Kanamycin from leaf explants were analyzed. For ‘Camiño Real’ the best shoot regeneration was established with a combination of 2.0 mg L-1 of TDZ + 0.1 mg L-1 of IBA. On the other hand, for ‘Festival’ the best shoot regeneration was established with a combination of 2.5 mg L-1 of TDZ + 0.1 mg L-1 of IBA. As far as Kanamycin sensitivity is concerned, total inhibition of the shoots was observed with 10 mg

  12. Transformations of the Pleiomorphic Plant MTOC during Sporogenesis in the Hepatic Marchantia polymorpha

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Sporogenesis in the hepatic Marchantia polymorpha L. provides an outstanding example of the pleiomorphic nature of the plant microtubule organizing center (MTOC). Microtubules are nucleated from γ-tubulin in MTOCs that change form during mitosis and meiosis. Following entry of cells into the reproductive pathway of sporogenesis, successive rounds of mitosis give rise to packets of 4-16 sporocytes. Mitotic spindles are organized at discrete polar organizers (POs), a type of MTOC that is unique to this group of early divergent land plants. An abrupt and radical transformation in microtubule organization occurs when sporocytes enter meiosis: POs are lost and γ-tubulin is closely associated with surfaces of two large elongated plastids that subsequently divide into four. Migration of the four plastid MTOCs into a tetrahedral arrangement establishes the future spore domains and the division polarity of meiosis. As is typical of many bryophytes,cones of microtubules from the four plastid MTOCs initiate a quadripolar microtubule system (QMS) in meiotic prophase.At this point a transformation in the organization of the MTOCs occurs. The γ-tubulin detaches from plastids and forms a diffuse spheroidal pole in each of the spore domains. The plastids, which are no longer MTOCs, continue to divide. The diffuse MTOCs continue to nucleate cones of microtubules during transformation of the QMS to a bipolar spindle. Following meiosis I, γ-tubulin is associated with nuclear envelopes, and the spindles of meiosis Ⅱ are organized from diffuse MTOCs at the tetrad poles. At simultaneous cytokinesis, radial microtubule systems are organized at nuclear envelope MTOCs in each of the tetrad members.

  13. Potassium chloride and rare earth elements improve plant growth and increase the frequency of the Agrobacterium tumefaciens-mediated plant transformation.

    Science.gov (United States)

    Boyko, Alex; Matsuoka, Aki; Kovalchuk, Igor

    2011-04-01

    Plant transformation efficiency depends on the ability of the transgene to successfully interact with plant host factors. Our previous work and the work of others showed that manipulation of the activity of host factors allows for increased frequency of transformation. Recently we reported that exposure of tobacco plants to increased concentrations of ammonium nitrate increases the frequency of both homologous recombination and plant transgenesis. Here we tested the influence of KCl and salts of rare earth elements, Ce and La on the efficiency of Agrobacterium-mediated plant transformation. We found that exposure to KCl, CeCl(3) and LaCl(3) leads to an increase in recombination frequency in Arabidopsis and tobacco. Plants grown in the presence of CeCl(3) and LaCl(3) had higher biomass, longer roots and greater root number. Analysis of transformation efficiency showed that exposure of tobacco plants to 50 mM KCl resulted in ~6.0-fold increase in the number of regenerated calli and transgenic plants as compared to control plants. Exposure to various concentrations of CeCl(3) showed a maximum increase of ~3.0-fold in both the number of calli and transgenic plants. Segregation analysis showed that exposure to KCl and cerium (III) chloride leads to more frequent integrations of the transgene at a single locus. Analysis of transgene intactness showed better preservation of right T-DNA border during transgene integration. Our data suggest that KCl and CeCl(3) can be effectively used to improve quantity and quality of transgene integrations.

  14. Mapping Soil Carbon from Cradle to Grave: C Transformations of Root Exudates and Plant Litter

    Science.gov (United States)

    Pett-Ridge, J.; Keiluweit, M.; Nuccio, E.; Bougoure, J.; Weber, P. K.; Brodie, E.; Mayali, X.; Shi, S.; Hwang, M.; Thelen, M.; Firestone, M.; Kleber, M.; Nico, P. S.

    2013-12-01

    Carbon cycling in the rhizosphere is a nexus of biophysical interactions between plant roots, microorganisms, and the soil organo-mineral matrix. Plant roots provide 30-40% of soil organic C inputs, accelerate the rate of organic matter mineralization by ~10X, and support an active microhabitat for microbial transformation of soil C. Our research on how roots influence decomposition of soil organic matter in both simplified and complex microcosms uses geochemical characterization, molecular microbiology, isotope tracing, metabolomics and novel imaging approaches (';ChipSIP' and ';STXM-SIMS') to trace the fate of isotopically labelled root exudates and plant tissues. Our previous work suggests root exudates drive O2 limitation, alter metal chemistry and mineralogy, and influence the availability of SOM. Our most recent experiments using synthetic rhizospheres were designed to identify the role of root exudates on ligno-cellulose decomposition in soils. Cultures of 13C/15N-labeled single plant cells (lignin-rich tracheary elements) were added to rhizosphere microcosm soils, and their decomposition followed under the influence of different root exudates using the dual imaging approach ';STXM-SIMS'. Using this combination of X-ray spectromicroscopy and NanoSIMS, we imaged the deconstruction of 13C/15N-labeled ligno-cellulose in situ, and mapped associations of plant cell-derived decomposition products with specific soil minerals. We've also looked at microbial community function in the more complex rhizospheres surrounding roots of the annual grass Avena fatua. Using an isotope array that allows us to follow root C into bacterial, fungal, and microfaunal communities, we tracked the movement of 13C from labeled exudates and 15N from labeled root litter into the soil microbial community. Our results indicate that the microbial communities involved in litter decomposition differ in rhizosphere versus bulk soils, which may have implications for carbon stabilization in soil.

  15. Use of a service evaluation and lean thinking transformation to redesign an NHS 111 refer to community Pharmacy for Emergency Repeat Medication Supply Service (PERMSS).

    Science.gov (United States)

    Nazar, Hamde; Nazar, Zachariah; Simpson, Jill; Yeung, Andre; Whittlesea, Cate

    2016-08-26

    To demonstrate the contribution of community pharmacy from NHS 111 referrals out of hours (OOH) for emergency supply repeat medication requests via presentation of service activity, community pharmacist feedback and lean thinking transformation. Descriptive service evaluation using routine service activity data over the pilot period; survey of community pharmacists, and service redesign through lean thinking transformation. North East of England NHS 111 provider and accredited community pharmacies across the North East of England. Patients calling the North East of England NHS 111 provider during OOH with emergency repeat medication supply requests. NHS 111 referral to community pharmacies for assessment and if appropriate, supply of emergency repeat medication. Number of emergency repeat medication supply referrals, completion rates, reasons for rejections, time of request, reason for access, medication(s), pharmaceutical advice and services provided. Secondary outcomes were community pharmacist feedback and lean thinking transformation of the patient pathway. NHS 111 referred 1468 patients to 114 community pharmacies (15/12/2014-7/4/2015). Most patients presented on Saturdays, with increased activity over national holidays. Community pharmacists completed 951 (64.8%) referrals providing 2297 medications; 412 were high risk. The most common reason for rejecting referrals was no medication in stock. Community pharmacists were positive about the provision of this service. The lean thinking transformation reduced the number of non-added value steps, waits and bottlenecks in the patient pathway. NHS 111 can redirect callers OOH from urgent and emergency care services to community pharmacy for management of emergency repeat medication supply. Existing IT and community pharmacy regulations allowed patients to receive a medication supply and pharmaceutical advice. Community pharmacists supported integration into the NHS OOH services. Adopting lean thinking provided a

  16. Identification of four Sedum plant medicines by Fourier transform infrared spectra

    Directory of Open Access Journals (Sweden)

    Ran Xu

    2012-01-01

    Full Text Available Background: Sedum sarmentosum bunge (SSB., S. lineare Thunb. (SLT, S. erythrostictum migo. (SEM, and S. aizoon L. (SAL were four widely used Chinese traditional drugs or ethnic drugs, which were easy to be confused with each other. Objective: This study aimed at developing a rapid and accurate method to identify the four Sedum plant medicines with very similar appearances and close relationships. Materials and Methods: The herbal medicines employed here were SSB, SLT, SEM, and SAL collected in different places and seasons. Through comparing the infrared (IR spectra of their 70% ethanol extracts, the results showed that the IR spectra of the four plant medicines possessed not only some common characteristics but also certain notable distinctions, such as shapes, numbers, positions, intensity, and ratios of the absorbing peaks. Results: By Fourier transform infrared (FT-IR spectroscopy, the four medicines could be effectively differed, their habitats could be judged preliminarily, and the genetic relationships of the original plants of the four medicines could also be estimated to some extent. Conclusion: The application of FT-IR spectroscopy in crude medicine authentication and quality evaluation deserved to be further emphasized.

  17. Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses

    Institute of Scientific and Technical Information of China (English)

    CHAI A-li; WANG Yi-kai; ZHU Fa-di; SHI Yan-xia; XIE Xue-wen; LI Bao-ju

    2016-01-01

    Identification of plant-pathogenic fungi is time-consuming due to cultivation and microscopic exami-nation and can be influenced by the interpretation of the micro-morphological characters observed.The present investigation aimed to create a simple but sophisticated method for the identification of plant-pathogenic fungi by Fourier transform infrared (FTIR)spectroscopy.In this study,FTIR-attenuated total reflectance (ATR) spectroscopy was used in combination with chemometric analysis for identification of important pathogenic fun-gi of horticultural plants.Mixtures of mycelia and spores from 27 fungal strains belonging to nine different families were collected from liquid PD or solid PDA media cultures and subj ected to FTIR-ATR spectroscopy measurements.The FTIR-ATR spectra ranging from 4 000 to 400 cm-1 were obtained.To classify the FTIR-ATR spectra,cluster analysis was compared with canonical vitiate analysis (CVA)in the spectral regions of 3 050~2 800 and 1 800~900 cm-1 .Results showed that the identification accuracies achieved 97.53% and 9 9.1 8% for the cluster analysis and CVA analysis,respectively,demonstrating the high potential of this tech-nique for fungal strain identification.

  18. High-efficiency Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) and regeneration of insect-resistant transgenic plants.

    Science.gov (United States)

    Mehrotra, Meenakshi; Sanyal, Indraneel; Amla, D V

    2011-09-01

    To develop an efficient genetic transformation system of chickpea (Cicer arietinum L.), callus derived from mature embryonic axes of variety P-362 was transformed with Agrobacterium tumefaciens strain LBA4404 harboring p35SGUS-INT plasmid containing the uidA gene encoding β-glucuronidase (GUS) and the nptII gene for kanamycin selection. Various factors affecting transformation efficiency were optimized; as Agrobacterium suspension at OD(600) 0.3 with 48 h of co-cultivation period at 20°C was found optimal for transforming 10-day-old MEA-derived callus. Inclusion of 200 μM acetosyringone, sonication for 4 s with vacuum infiltration for 6 min improved the number of GUS foci per responding explant from 1.0 to 38.6, as determined by histochemical GUS assay. For introducing the insect-resistant trait into chickpea, binary vector pRD400-cry1Ac was also transformed under optimized conditions and 18 T(0) transgenic plants were generated, representing 3.6% transformation frequency. T(0) transgenic plants reflected Mendelian inheritance pattern of transgene segregation in T(1) progeny. PCR, RT-PCR, and Southern hybridization analysis of T(0) and T(1) transgenic plants confirmed stable integration of transgenes into the chickpea genome. The expression level of Bt-Cry protein in T(0) and T(1) transgenic chickpea plants was achieved maximum up to 116 ng mg(-1) of soluble protein, which efficiently causes 100% mortality to second instar larvae of Helicoverpa armigera as analyzed by an insect mortality bioassay. Our results demonstrate an efficient and rapid transformation system of chickpea for producing non-chimeric transgenic plants with high frequency. These findings will certainly accelerate the development of chickpea plants with novel traits.

  19. Transgenic plants from shoot apical meristems of Vitis vinifera L. "Thompson Seedless" via Agrobacterium-mediated transformation.

    Science.gov (United States)

    Dutt, M; Li, Z T; Dhekney, S A; Gray, D J

    2007-12-01

    Shoot apical meristem explants of Vitis vinifera "Thompson Seedless" were used for Agrobacterium-mediated genetic transformation. It was determined that the meristems had to be subjected to a dark growth phase then wounded to obtain transgenic plants. Morphological and histological studies illustrated the role of wounding to expose apical meristem cells for transformation. A bifunctional egfp/nptII fusion gene was used to select kanamycin resistant plants that expressed green fluorescent protein (GFP). Kanamycin at a concentration of 16 mg L(-1) in selection medium resulted in recovery of non-chimeric transgenic plants that uniformly expressed GFP, whereas 8 mg L(-1) kanamycin allowed non-transgenic and/or chimeric plants to develop. Polymerase chain reaction (PCR) and Southern blot analyses confirmed the presence of transgenes and their stable integration into the genome of regenerated plants. Up to 1% of shoot tips produced stable transgenic cultures within 6 weeks of treatment, resulting in a total of 18 independent lines.

  20. USING OF Agrobacterium-MEDIATED TRANSFORMATION FOR THE BIOTECHNOLOGICAL IMPROVEMENT OF COMPOSITAE PLANTS. ІІ. SYNTHESIS OF BIOACTIVE COMPOUNDS IN TRANSGENIC PLANTS AND «HAIRY» ROOTS

    Directory of Open Access Journals (Sweden)

    N. A. Matvieieva

    2015-04-01

    Full Text Available The review focused on the data concerning current state in the field of Compositae “hairy” roots and transgenic plants construction using A.tumefaciens- and A. rhizogenes-mediated transformation to obtain biologically active compounds, including recombinant proteins. The article presents data on the results of genetic transformation of Cichorium intybus, Lactuca sativa, Artemisia annua, Artemisia vulgaris, Calendula officinalis, Withania somnifera and other Compositae plants as well as studies on the artemisinin, flavonoids, polyphenols, fructans and other compounds accumulation in transgenic plants and roots. The data show that the use of biotechnological approaches for construction of "hairy" roots and transgenic plants with new features are of great interest. The possibility of increase in the accumulation of naturally synthesized bioactive compounds and recombinant proteins production via A. tumefaciens and A. rhizogenes-mediated transformation have been shown. In vitro cultivation of transgenic plants characterized by high level of bioactive compounds accumulation and synthesis of recombinant proteins makes it possible to obtain guaranteed pure raw material. Using of biotechnological approaches preserved natural populations of plants is particularly important for rare and endangered plant species.

  1. Environmental Protection Agency (EPA) Facility Registry Service (FRS) Wastewater Treatment Plants

    Data.gov (United States)

    Department of Homeland Security — This GIS dataset contains data on wastewater treatment plants, based on EPA's Facility Registry Service (FRS) and NPDES, along with Clean Watersheds Needs Survey...

  2. Application of generalized Hough transform for detecting sugar beet plant from weed using machine vision method

    Directory of Open Access Journals (Sweden)

    A Bakhshipour Ziaratgahi

    2017-05-01

    Full Text Available Introduction Sugar beet (Beta vulgaris L. as the second most important world’s sugar source after sugarcane is one of the major industrial crops. The presence of weeds in sugar beet fields, especially at early growth stages, results in a substantial decrease in the crop yield. It is very important to efficiently eliminate weeds at early growing stages. The first step of precision weed control is accurate detection of weeds location in the field. This operation can be performed by machine vision techniques. Hough transform is one of the shape feature extraction methods for object tracking in image processing which is basically used to identify lines or other geometrical shapes in an image. Generalized Hough transform (GHT is a modified version of the Hough transform used not only for geometrical forms, but also for detecting any arbitrary shape. This method is based on a pattern matching principle that uses a set of vectors of feature points (usually object edge points to a reference point to construct a pattern. By comparing this pattern with a set pattern, the desired shape is detected. The aim of this study was to identify the sugar beet plant from some common weeds in a field using the GHT. Materials and Methods Images required for this study were taken at the four-leaf stage of sugar beet as the beginning of the critical period of weed control. A shelter was used to avoid direct sunlight and prevent leaf shadows on each other. The obtained images were then introduced to the Image Processing Toolbox of MATLAB programming software for further processing. Green and Red color components were extracted from primary RGB images. In the first step, binary images were obtained by applying the optimal threshold on the G-R images. A comprehensive study of several sugar beet images revealed that there is a unique feature in sugar beet leaves which makes them differentiable from the weeds. The feature observed in all sugar beet plants at the four

  3. Karhunen-Loeve Transform and Sparse Representation Based Plant Leaf Disease Recognition

    Directory of Open Access Journals (Sweden)

    Tian Jie

    2013-09-01

    Full Text Available To improve the classification accuracy rate of apple leaf disease images and solve the problem of dimension redundancy in feature extraction, Karhunen-Loeve (K-L transform and sparse representation are applied to apple leaf disease recognition. Firstly 9 color features and 8 texture features of disease leaf images are extracted and taken as feature vectors after dimensionality reduction by the K-L transform. Then, for each of apple mosaic virus, apple rust and apple alternaria leaf spot, 40 apple leaf images are selected as the training samples, whose feature vectors are made up of the dictionary of the sparse representation, respectively. Each testing sample is classified into the class with the minimal residual. The identifying results using the proposed method are analyzed and compared with those of the Support Vector Machine (SVM and original sparse representation method. The average classification accuracy rate of the proposed method is 94.18 %, which confirms its good robustness. In addition, the proposed method not only improves the plant leaf disease classification accuracy but also solves the redundancy problem of the extracted features.

  4. Where to plant urban trees? A spatially explicit methodology to explore ecosystem service tradeoffs

    Science.gov (United States)

    E.W. Bodnaruk; C.N. Kroll; Y. Yang; S. Hirabayashi; David Nowak; T.A. Endreny

    2017-01-01

    Urban trees can help mitigate some of the environmental degradation linked to the rapid urbanization of humanity. Many municipalities are implementing ambitious tree planting programs to help remove air pollution, mitigate urban heat island effects, and provide other ecosystem services and benefits but lack quantitative tools to explore priority planting locations and...

  5. Service user involvement in practitioner education: Movement politics and transformative change.

    Science.gov (United States)

    Mckeown, Mick; Dix, Julie; Jones, Fiona; Carter, Bernie; Malihi-Shoja, Lisa; Mallen, Ernie; Harrison, Nigel

    2014-08-01

    This paper will attempt to celebrate both key developments and best practice involving the users of health and social care services in programmes of practitioner education in a UK context, and offer a critical appraisal of the extent to which such initiatives meet some of the more transformative objectives sought by service user activists for change. The approach is largely that of a discussion paper but we will illustrate some of the themes relating to movement activism with selected data. These data relate to earlier research and two specially convened focus groups within the Comensus initiative at the University of Central Lancashire; itself constituted as a piece of participatory action research. We conclude that universities represent paradoxical sites for the facilitation of debate and learning relevant to key issues of social justice and change. As such, they are places that can impede or support movement aims. Particular strategic responses might be more likely to engender progressive outcomes. These ought to include the presence of critically engaged academic staff operating within a scholarly culture that fosters forms of deliberative democratic decision making.

  6. Provision of Continuity in Power Supply of Wind Power Stations by Improvement of Methods for Control of Oil Transformer Serviceability

    Directory of Open Access Journals (Sweden)

    Y. A. Rolik

    2010-01-01

    Full Text Available The paper considers problems pertaining to reduction of time for restoration of a wind power station (WPS by development of a method and control means according to informative parameter. A high-voltage oil transformer has been selected as a control WPS object.  The paper shows that the most informative parameter determining continuity in WPS power supply is an over-heating temperature of oil transformer elements. A method and means for control of WPS oil transformer serviceability are proposed in the paper.

  7. Refueling outage services in Spanish Nuclear Power Plants; Servicios en recargas de centrales nucleares

    Energy Technology Data Exchange (ETDEWEB)

    Landete, J. L.; Soto, M.; Nunuez, A.

    2007-07-01

    DOMINGUIS Group, through its 75 years of business development, has positioned as the Spanish leader Group in Services for the Nuclear Energy and Petrochemical Sectors. In this article, we present the most significant services summary that, through the companies that constitute DOMINGUIS Group, we have developed in Refueling Outage in Spanish Nuclear Power Plants. (Author)

  8. 47 CFR 32.6561 - Depreciation expense-telecommunications plant in service.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Depreciation expense-telecommunications plant in service. 32.6561 Section 32.6561 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES UNIFORM SYSTEM OF ACCOUNTS FOR TELECOMMUNICATIONS COMPANIES Instructions...

  9. Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology.

    Science.gov (United States)

    Ishizaki, Kimitsune; Chiyoda, Shota; Yamato, Katsuyuki T; Kohchi, Takayuki

    2008-07-01

    Agrobacterium-mediated transformation has not been practical in pteridophytes, bryophytes and algae to date, although it is commonly used in model plants including Arabidopsis and rice. Here we present a rapid Agrobacterium-mediated transformation system for the haploid liverwort Marchantia polymorpha L. using immature thalli developed from spores. Hundreds of hygromycin-resistant plants per sporangium were obtained by co-cultivation of immature thalli with Agrobacterium carrying the binary vector that contains a reporter, the beta-glucuronidase (GUS) gene with an intron, and a selection marker, the hygromycin phosphotransferase (hpt) gene. In this system, individual gemmae, which arise asexually from single initial cells, were analyzed as isogenic transformants. GUS activity staining showed that all hygromycin-resistant plants examined expressed the GUS transgene in planta. DNA analyses verified random integration of 1-5 copies of the intact T-DNA between the right and the left borders into the M. polymorpha genome. The efficient and rapid Agrobacterium-mediated transformation of M. polymorpha should provide molecular techniques to facilitate comparative genomics, taking advantage of this unique model plant that retains many features of the common ancestor of land plants.

  10. Acquiring transgenic tobacco plants with insect resistance and glyphosate tolerance by fusion gene transformation.

    Science.gov (United States)

    Sun, He; Lang, Zhihong; Zhu, Li; Huang, Dafang

    2012-10-01

    The advantages of gene 'stacking' or 'pyramiding' are obvious in genetically modified (GM) crops, and several different multi-transgene-stacking methods are available. Using linker peptides for multiple gene transformation is considered to be a good method to meet a variety of needs. In our experiment, the Bt cry1Ah gene, which encodes the insect-resistance protein, and the mG ( 2 ) -epsps gene, which encodes the glyphosate-tolerance protein, were connected by a 2A or LP4/2A linker. Linker 2A is a peptide from the foot-and-mouth disease virus (FMDV) that has self-cleavage activity. LP4 is a peptide from Raphanus sativus seeds that has a recognition site and is cleaved by a protease. LP4/2A is a hybrid peptide that contains the first 9 amino acids of LP4 and 20 amino acids from 2A. We used the linker peptide to construct four coordinated expression vectors: pHAG, pHLAG, pGAH and pGLAH. Two single gene expression vectors, pSAh and pSmG(2), were used as controls. The six expression vectors and the pCAMBIA2301 vector were transferred into tobacco by Agrobacterium tumefaciens-mediated transformation, and 529 transformants were obtained. Molecular detection and bioassay detection data demonstrated that the transgenic tobaccos possessed good pest resistance and glyphosate tolerance. The two genes in the fusion vector were expressed simultaneously. The plants with the genes linked by the LP4/2A peptide showed better pest resistance and glyphosate tolerance than the plants with the genes linked by 2A. The expression level of the two genes linked by LP4/2A was not significantly different from the single gene vector. Key message The expression level of the two genes linked by LP4/2A was higher than those linked by 2A and was not significantly different from the single gene vector.

  11. ANTIOXIDANT PROPERTIES OF METHANOLIC EXTRACTS FROM THE SHOOTS AND ROOTS OF pRi-TRANSFORMED PLANTS OF REHMANNIA GLUTINOSA LIBOSCH.

    Science.gov (United States)

    Piatczak, Ewelina; Dfbska, Marta; Kontek, Bogdan; Olas, Beata; Wysokinskai, Halina

    2016-01-01

    The antioxidant activity of methanolic extracts derived from shoots (HR-shoots) and roots (HR-roots) of pRi-transformed Rehmannia glutinosa plants were determined. The activity was indicated by the ability of the plant extracts to inhibit superoxide anion (O2(-·)) generation and thiobarbituric acid reactive substances (TBARS) production in resting blood platelets and platelets activated by thrombin. The strongest activity was exhibited by the HR-shoot extract (50 μg/mL). The present study also examines the antioxidant properties of the plant extracts against human plasma lipid peroxidation induced by strong biological oxidants: hydrogen peroxide (H2O2) and H2O2/Fe. The study shows that extracts from transformed R. glutinosa plants may be a promising source of natural antioxidants, which would be valuable in various cardiovascular diseases. The extracts may also protect lipids against oxidative modifications.

  12. Alkene Metathesis Catalysis: A Key for Transformations of Unsaturated Plant Oils and Renewable Derivatives

    Directory of Open Access Journals (Sweden)

    Dixneuf Pierre H.

    2016-03-01

    Full Text Available This account presents the importance of ruthenium-catalysed alkene cross-metathesis for the catalytic transformations of biomass derivatives into useful intermediates, especially those developed by the authors in the Rennes (France catalysis team in cooperation with chemical industry. The cross-metathesis of a variety of functional alkenes arising from plant oils, with acrylonitrile and fumaronitrile and followed by catalytic tandem hydrogenation, will be shown to afford linear amino acid derivatives, the precursors of polyamides. The exploration of cross-metathesis of bio-sourced unsaturated nitriles with acrylate with further catalytic hydrogenation has led to offer an excellent route to α,ω-amino acid derivatives. That of fatty aldehydes has led to bifunctional long chain aldehydes and saturated diols. Two ways of access to functional dienes by ruthenium-catalyzed ene-yne cross-metathesis of plant oil alkene derivatives with alkynes and by cross-metathesis of bio-sourced alkenes with allylic chloride followed by catalytic dehydrohalogenation, are reported. Ricinoleate derivatives offer a direct access to chiral dihydropyrans and tetrahydropyrans via ring closing metathesis. Cross-metathesis giving value to terpenes and eugenol for the straightforward synthesis of artificial terpenes and functional eugenol derivatives without C=C bond isomerization are described.

  13. Sulfate removal and sulfur transformation in constructed wetlands: The roles of filling material and plant biomass.

    Science.gov (United States)

    Chen, Yi; Wen, Yue; Zhou, Qi; Huang, Jingang; Vymazal, Jan; Kuschk, Peter

    2016-10-01

    Sulfate in effluent is a challenging issue for wastewater reuse around the world. In this study, sulfur (S) removal and transformation in five batch constructed wetlands (CWs) treating secondary effluent were investigated. The results showed that the presence of the plant cattail (Typha latifolia) had little effect on sulfate removal, while the carbon-rich litter it generated greatly improved sulfate removal, but with limited sulfide accumulation in the pore-water. After sulfate removal, most of the S was deposited with the valence states S (-II) and S (0) on the iron-rich gravel surface, and acid volatile sulfide was the main S sink in the litter-added CWs. High-throughput pyrosequencing revealed that sulfate-reducing bacteria (i.e. Desulfobacter) and sulfide-oxidizing bacteria (i.e. Thiobacillus) were dominant in the litter-added CWs, which led to a sustainable S cycle between sulfate and sulfide. Overall, this study suggests that recycling plant litter and iron-rich filling material in CWs gives an opportunity to utilize the S in the wastewater as both an electron acceptor for sulfate reduction and as an electron donor for nitrate reduction coupled with sulfide oxidation. This leads to the simultaneous removal of sulfate, nitrate, and organics without discharging toxic sulfide into the receiving water body. Copyright © 2016 Elsevier Ltd. All rights reserved.

  14. Impact of high strength electromagnetic fields generated by Tesla transformer on plant cell ultrastructure

    Directory of Open Access Journals (Sweden)

    Anna Rusakova

    2017-09-01

    Full Text Available Non-thermal effects of direct electric fields and alternating electromagnetic fields (EMF have been successfully used in a number of studies and applications in agriculture and biotechnology. Among different kinds of high strength EMF generators, the Tesla transformer (TT is known as a widely applied, low cost, and troubleproof device, which generates EMF in the range of 2–8 MHz. Despite of a number of developed and perspective applications of high strength EMFs in agriculture and biotechnology, the EMFs generated by TT, as well as the 1–50 MHz range of high strength EMF still remain unexplored in the fields of plant physiology, ultrastructure studies and biochemistry. In this work, we have shown that TT-EMFs (4 MHz induced fast stem and petiole bending, disappearance of cell organelles, vacuolar membranes, and increase of a non-photochemical chlorophyll fluorescence quenching in petioles. It is intriguing that such fatal effects can be evoked in plants by EMFs which are well known as harmless for man at the applied strength and frequency.

  15. Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics

    Directory of Open Access Journals (Sweden)

    Liu Wei

    2009-06-01

    Full Text Available Abstract Background Transgenic approaches provide a powerful tool for gene function investigations in plants. However, some legumes are still recalcitrant to current transformation technologies, limiting the extent to which functional genomic studies can be performed on. Superroot of Lotus corniculatus is a continuous root cloning system allowing direct somatic embryogenesis and mass regeneration of plants. Recently, a technique to obtain transgenic L. corniculatus plants from Superroot-derived leaves through A. tumefaciens-mediated transformation was described. However, transformation efficiency was low and it took about six months from gene transfer to PCR identification. Results In the present study, we developed an A. rhizogenes-mediated transformation of Superroot-derived L. corniculatus for gene function investigation, combining the efficient A. rhizogenes-mediated transformation and the rapid regeneration system of Superroot. The transformation system using A. rhizogenes K599 harbouring pGFPGUSPlus was improved by validating some parameters which may influence the transformation frequency. Using stem sections with one node as explants, a 2-day pre-culture of explants, infection with K599 at OD600 = 0.6, and co-cultivation on medium (pH 5.4 at 22°C for 2 days enhanced the transformation frequency significantly. As proof of concept, Superroot-derived L. corniculatus was transformed with a gene from wheat encoding an Na+/H+ antiporter (TaNHX2 using the described system. Transgenic Superroot plants were obtained and had increased salt tolerance, as expected from the expression of TaNHX2. Conclusion A rapid and efficient tool for gene function investigation in L. corniculatus was developed, combining the simplicity and high efficiency of the Superroot regeneration system and the availability of A. rhizogenes-mediated transformation. This system was improved by validating some parameters influencing the transformation frequency, which could

  16. Transformation of traditional knowledge of medicinal plants: the case of Tyroleans (Austria) who migrated to Australia, Brazil and Peru

    OpenAIRE

    Pirker Heidemarie; Haselmair Ruth; Kuhn Elisabeth; Schunko Christoph; Vogl Christian R

    2012-01-01

    Abstract Background In ethnobotanical research, the investigation into traditional knowledge of medicinal plants in the context of migration has been of increasing interest in recent decades since it is influenced and changed by new environmental and social conditions. It most likely undergoes transformation processes to match the different living circumstances in the new location. This study compares the traditional knowledge of medicinal plants held by Tyroleans – and their descendants – wh...

  17. Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?

    Science.gov (United States)

    Chovelon, V; Restier, V; Giovinazzo, N; Dogimont, C; Aarrouf, J

    2011-11-01

    Melon (Cucumis melo L.) is widely considered as a recalcitrant species for genetic transformation. In this study, we developed different regeneration and transformation protocols and we examined the regeneration process at different steps by histological studies. The highest regeneration rate (1.13 ± 0.02 plants per explant) was obtained using cotyledon explants of the 'Védrantais' genotype on Murashige and Skoog (MS) medium supplemented with 0.2 mg/l 6-benzylaminopurine (BAP) and 0.2 mg/l dimethylallylaminopurine (2-iP). Agrobacterium tumefaciens-mediated transformations with the uidA reporter gene were realized on cotyledon explants cultivated in these conditions: 70-90% of explants expressed a transient GUS activity during the early stages of regeneration, however, only few transgenic plants were obtained (1.8-4.5% of stable transformation with the GV2260pBI101 strain). These results revealed a low capacity of melon GUS-positive cells to regenerate transgenic plants. To evaluate the influence of the Agrobacterium infection on plant regeneration, histological analyses were conducted on explants 2, 7, 15, and 28 days after co-culture with the GV2260pBI101 strain. Genetic transformation occurred in epidermal and sub-epidermal cells and reached the meristematic structures expressing a high level of GUS activity during 14 days of culture; but after this period, most of the meristematic structures showed premature cell vacuolization and disorganization. This disruption of the GUS-positive meristematic areas could be responsible of the difficulties encountered to regenerate melon plants after genetic transformation.

  18. An efficient method for Agrobacterium-mediated genetic transformation and plant regeneration in cumin (Cuminum cyminum L.).

    Science.gov (United States)

    Pandey, Sonika; Mishra, Avinash; Patel, Manish Kumar; Jha, Bhavanath

    2013-09-01

    Cumin is an annual herbaceous medicinally important plant having diverse applications. An efficient and reproducible method of Agrobacterium-mediated genetic transformation was herein established for the first time. A direct regeneration method without callus induction was optimised using embryos as explant material in Gamborg's B5 medium supplemented with 0.5-μM 6-benzyladenine and 2.0-μM α-naphthalene acetic acid. About 1,020 embryos (a mean of 255 embryos per batch) were used for the optimisation of transformation conditions. These conditions were an Agrobacterium cell suspension of 0.6 OD600, a co-cultivation time of 72 h, 300-μM acetosyringone and wounding of explants using a razor blade. Pre-cultured elongated embryos were treated using optimised conditions. About 720 embryos (a mean of 180 embryos per batch) were used for transformation and 95 % embryos showed transient β-glucuronidase expression after co-cultivation. Putative transformed embryos were cultured on B5 medium for shoot proliferation and 21 regenerated plants were obtained after selection and allowed to root. T0 plantlets showed β-glucuronidase expression and gene integration was confirmed via PCR amplification of 0.96 and 1.28 kb fragments of the hygromycin-phosphotransferase II and β-glucuronidase genes, respectively. In this study, a transformation efficiency of 1.5 % was demonstrated and a total of 11 transgenic plants were obtained at the hardening stage, however, only four plants acclimatised during hardening. Gene copy number was analysed by Southern blot analysis of hardened plants and single-copy gene integration was observed. This is the first successful attempt of Agrobacterium-mediated genetic transformation of cumin.

  19. 1996-97 Buying Guide - Power Plant Products and Services

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-09-01

    This buyers guide for the electric power industry contains a product index and directory, a services index and directory, and a company listing (gives the company name and address, and where possible the telephone and fax number and e-mailing addresses).

  20. Directly Transforming PCR-Amplified DNA Fragments into Plant Cells Is a Versatile System That Facilitates the Transient Expression Assay

    Science.gov (United States)

    Lu, Yuming; Chen, Xi; Wu, Yuxuan; Wang, Yanping; He, Yuqing; Wu, Yan

    2013-01-01

    A circular plasmid containing a gene coding sequence has been broadly used for studying gene regulation in cells. However, to accommodate a quick screen plasmid construction and preparation can be time consuming. Here we report a PCR amplified dsDNA fragments (PCR-fragments) based transient expression system (PCR-TES) for suiting in the study of gene regulation in plant cells. Instead of transforming plasmids into plant cells, transient expression of PCR-fragments can be applicable. The transformation efficiency and expression property of PCR-fragments are comparable to transformation using plasmids. We analyzed the transformation efficiency in PCR-TES at transcription and protein levels. Our results indicate that the PCR-TES is as versatile as the conventional transformation system using plasmid DNA. Through reconstituting PYR1-mediated ABA signaling pathway in Arabidopsis mesophyll protoplasts, we were not only validating the practicality of PCR-TES but also screening potential candidates of CDPK family members which might be involved in the ABA signaling. Moreover, we determined that phosphorylation of ABF2 by CPK4 could be mediated by ABA-induced PYR1 and ABI1, demonstrating a crucial role of CDPKs in the ABA signaling. In summary, PCR-TES can be applicable to facilitate analyzing gene regulation and for the screen of putative regulatory molecules at the high throughput level in plant cells. PMID:23468926

  1. Directly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay.

    Directory of Open Access Journals (Sweden)

    Yuming Lu

    Full Text Available A circular plasmid containing a gene coding sequence has been broadly used for studying gene regulation in cells. However, to accommodate a quick screen plasmid construction and preparation can be time consuming. Here we report a PCR amplified dsDNA fragments (PCR-fragments based transient expression system (PCR-TES for suiting in the study of gene regulation in plant cells. Instead of transforming plasmids into plant cells, transient expression of PCR-fragments can be applicable. The transformation efficiency and expression property of PCR-fragments are comparable to transformation using plasmids. We analyzed the transformation efficiency in PCR-TES at transcription and protein levels. Our results indicate that the PCR-TES is as versatile as the conventional transformation system using plasmid DNA. Through reconstituting PYR1-mediated ABA signaling pathway in Arabidopsis mesophyll protoplasts, we were not only validating the practicality of PCR-TES but also screening potential candidates of CDPK family members which might be involved in the ABA signaling. Moreover, we determined that phosphorylation of ABF2 by CPK4 could be mediated by ABA-induced PYR1 and ABI1, demonstrating a crucial role of CDPKs in the ABA signaling. In summary, PCR-TES can be applicable to facilitate analyzing gene regulation and for the screen of putative regulatory molecules at the high throughput level in plant cells.

  2. Water mediated alterations in gravity signal transform phytofilertation capability in hydroponic plants

    Science.gov (United States)

    Singh, Yogranjan; Singh Marabi, Rakesh; Satpute, Gyanesh Kumar; Mishra, Stuti

    2012-07-01

    An exorbitant sum of different synthetic molecules of chemicals including dyes and pigments are discharged into the environment, mainly via industrial effluents every year worldwide. The physical-chemical treatments for remediation viz adsorption, precipitation, ion exchange or filtration have proved to be disadvantageous because of high cost, low efficiency and inapplicability to a wide variety of dyes, or the formation of by-products and thereby creating waste disposal problems. Similarly the limited ability of micro-organisms to degrade xenobiotic especially sulphonoaromatic compounds, limits the efficiency and, therefore, the use of conventional wastewater treatment plants. In this context, the development of alternative biological treatments to eliminate these pollutants from industrial effluents is an important requirement. Plant metabolism, is extremely diverse and can be exploited to treat recalcitrant pollutants, not degradable by bacteria or fungi and can act as an important global sink for environmental pollutants. The presence of putative metabolites, in leaves of hydrophytes has been observed, indicating the transformation of several xenobiotics. A diverse range of the enzymes involved in the early stages of the detoxification process are closely associated with the redox biochemistry of the cell. The activities of enzymes such as glutathione transferases, peroxidases and cytochrome P450 monooxygenases and its multigenic family have implications with respect to the maintenance of redox homeostasis. Besides activating xenobiotics, cytochromes P450 is involved vitally in cell signaling for counteracting buoyant balance. Signal transduction cascades, including the role of cytochrome P450 monooxygenases in responding to gravitational cues, appear to be affected by buoyancy as well. Gravitropism is the orientation of growth in response to gravity and involves the perception of the gravitational force in the columella cells of the root cap where the primary

  3. Impact of microbial/plant interactions on the transformation of polycyclic aromatic hydrocarbons in rhizosphere of Festuca arundinacea.

    Science.gov (United States)

    Ho, C H; Applegate, B; Banks, M K

    2007-01-01

    The promotion of polycyclic aromatic hydrocarbon (PAH) degradation was demonstrated in the rhizosphere of Festuca arundinacea with Pseudomonas fluorescens. P. fluorescens 5RL more significantly interacted with salicylate and dextrose in the agar containing tall fescue than agar without plant roots. Although the presence of tall fescue did not promote catabolic enzyme induction in the absence of salicylate, an increase in dioxygenase activity relative to no plant controls implies that this plant may enhance the degradation of PAHs or facilitate the genotypes that are capable of transforming PAH in the rhizosphere.

  4. ICT-Platform to Transform Car Dealerships to Regional Providers of Sustainable Mobility Services

    Directory of Open Access Journals (Sweden)

    Benjamin Wagner vom Berg

    2017-02-01

    Full Text Available Aim/Purpose: The topic of this study is the ICT-enabled transformation of car dealerships to regional providers of sustainable mobility (e.g., car sharing. Background: Car dealerships offer specific conditions that enable a sustainable mobility offer, based on individual motorized transport like car sharing. This is especially useful in small towns or rural areas where people’s mobility is strongly dominated by private cars, and public transport coverage is limited. However, these new mobility services are combined services with the need of a deep integration of information systems, and these services are not yet related to car dealerships and customer acquisition has to be supported. Methodology: An empirical study with an inductive approach was chosen. The study consists of interviews with three focus groups of different stakeholders of car dealerships. Within the frame of the research project, “ReCaB – Regional Car-Balancing” a qualitative research approach was chosen. Within a design science approach the existing SusCRM architecture was adapted based on the elaborated requirements. Contribution: A software architecture is proposed, where Customer Relationship Management (CRM components to market new sustainable mobility offers are vital parts and existing information systems of car dealerships are integrated. Findings: The basic feasibility of the establishment and customer acceptance has been demonstrated, at least in the area of car sharing within ReCaB. The execution in the car sharing field has already started and a number of car dealerships are already bringing their own offers to market. Major findings for the SusCRM architecture have been elaborated in a design science approach in the national German research project “Showcase for electro mobility”. Recommendations for Practitioners: There is still no fully functional prototype developed for this specific use case and evaluated in the car dealership environment. An

  5. Natural transformation in plant breeding - a biotechnological platform for quality improvement of ornamental, agricultural and medicinal plants

    DEFF Research Database (Denmark)

    Lütken, Henrik Vlk; Hegelund, Josefine Nymark; Himmelboe, Martin;

    2015-01-01

    Compactness is a desirable trait in ornamental plant breeding because it is preferred by producers, distributors and consumers. Presently, in ornamental plant production growth of many potted plants is regulated by application of chemical growth retardants, several of which are harmful to both......, decreased plant height, short internodes, reduced apical dominance and changes in flower characteristics. Several of these traits improve ornamental plant quality and may also benefit characteristics useful in agricultural field crops. In addition, a number of regenerated plants derived from hairy roots...

  6. Water quality transformations during soil aquifer treatment at the Mesa Northwest Water Reclamation Plant, USA.

    Science.gov (United States)

    Fox, P; Narayanaswamy, K; Genz, A; Drewes, J E

    2001-01-01

    Water quality transformations during soil aquifer treatment at the Mesa Northwest Water Reclamation Plant (NWWRP) were evaluated by sampling a network of groundwater monitoring wells located within the reclaimed water plume. The Mesa Northwest Water Reclamation Plant has used soil aquifer treatment (SAT) since it began operation in 1990 and the recovery of reclaimed water from the impacted groundwater has been minimal. Groundwater samples obtained represent travel times from several days to greater than five years. Samples were analyzed for a wide range of organic and inorganic constituents. Sulfate was used as a tracer to estimate travel times and define reclaimed water plume movement. Dissolved organic carbon concentrations were reduced to approximately 1 mg/L after 12 to 24 months of soil aquifer treatment with an applied DOC concentration from the NWWRP of 5 to 7 mg/L. The specific ultraviolet absorbance (SUVA) increased during initial soil aquifer treatment on a time-scale of days and then decreased as longer term soil aquifer treatment removed UV absorbing compounds. The trihalomethane formation potential (THMFP) was a function of the dissolved organic carbon concentration and ranged from 50 to 65 micrograms THMFP/mg DOC. Analysis of trace organics revealed that the majority of trace organics were removed as DOC was removed with the exception of organic iodine. The majority of nitrogen was applied as nitrate-nitrogen and the reclaimed water plume had lower nitrate-nitrogen concentrations as compared to the background groundwater. The average dissolved organic carbon concentrations in the reclaimed water plume were less than 50% of the drinking water dissolved organic concentrations from which the reclaimed water originated.

  7. Effects of Invasive-Plant Management on Nitrogen-Removal Services in Freshwater Tidal Marshes.

    Science.gov (United States)

    Alldred, Mary; Baines, Stephen B; Findlay, Stuart

    2016-01-01

    Establishing relationships between biodiversity and ecosystem function is an ongoing endeavor in contemporary ecosystem and community ecology, with important practical implications for conservation and the maintenance of ecosystem services. Removal of invasive plant species to conserve native diversity is a common management objective in many ecosystems, including wetlands. However, substantial changes in plant community composition have the potential to alter sediment characteristics and ecosystem services, including permanent removal of nitrogen from these systems via microbial denitrification. A balanced assessment of costs associated with keeping and removing invasive plants is needed to manage simultaneously for biodiversity and pollution targets. We monitored small-scale removals of Phragmites australis over four years to determine their effects on potential denitrification rates relative to three untreated Phragmites sites and adjacent sites dominated by native Typha angustifolia. Sediment ammonium increased following the removal of vegetation from treated sites, likely as a result of decreases in both plant uptake and nitrification. Denitrification potentials were lower in removal sites relative to untreated Phragmites sites, a pattern that persisted at least two years following removal as native plant species began to re-colonize treated sites. These results suggest the potential for a trade-off between invasive-plant management and nitrogen-removal services. A balanced assessment of costs associated with keeping versus removing invasive plants is needed to adequately manage simultaneously for biodiversity and pollution targets.

  8. Effects of Invasive-Plant Management on Nitrogen-Removal Services in Freshwater Tidal Marshes.

    Directory of Open Access Journals (Sweden)

    Mary Alldred

    Full Text Available Establishing relationships between biodiversity and ecosystem function is an ongoing endeavor in contemporary ecosystem and community ecology, with important practical implications for conservation and the maintenance of ecosystem services. Removal of invasive plant species to conserve native diversity is a common management objective in many ecosystems, including wetlands. However, substantial changes in plant community composition have the potential to alter sediment characteristics and ecosystem services, including permanent removal of nitrogen from these systems via microbial denitrification. A balanced assessment of costs associated with keeping and removing invasive plants is needed to manage simultaneously for biodiversity and pollution targets. We monitored small-scale removals of Phragmites australis over four years to determine their effects on potential denitrification rates relative to three untreated Phragmites sites and adjacent sites dominated by native Typha angustifolia. Sediment ammonium increased following the removal of vegetation from treated sites, likely as a result of decreases in both plant uptake and nitrification. Denitrification potentials were lower in removal sites relative to untreated Phragmites sites, a pattern that persisted at least two years following removal as native plant species began to re-colonize treated sites. These results suggest the potential for a trade-off between invasive-plant management and nitrogen-removal services. A balanced assessment of costs associated with keeping versus removing invasive plants is needed to adequately manage simultaneously for biodiversity and pollution targets.

  9. Ca2+ transport in plant cells and mechanisms of transformation of phytochrome-induced photosignals

    Science.gov (United States)

    Volotovski, Igor D.

    1995-01-01

    The recent data on the influence of phytochrome on the efficiency of Ca2+ translocation across the membranes of oat protoplasts are given. Ca2+ uptake in the protoplasts was shown to be influenced by the red light (R) illumination. This effect was reverted by the following far-red light (FR) illumination. To elucidate the sensitivity to phytochrome-controlling action the screening between the mechanisms of Ca2+ transport across the plasma membranes of oat protoplasts, Na+/Ca2+ and Ca2+/H+ exchangers, Ca2+-pump and Ca2+-channel was done. It was established that phytochrome modulated the activity of Na+/Ca2+-exchanger and Ca2+-pump. The light-mediated oscillations of cytoplasmic Ca2+ concentration in the oat protoplasts were demonstrated using fluorescence probe quin2 loaded into the cells and laser monitoring of fluorescence signal. The evidences were obtained that the oscillations were not the result of the elevation of cytoplasmic Ca2+ concentration and had no connection with Ca2+ pool of mitochondria. The possibility of the relation between the Ca2+ oscillations and phosphoinositide metabolism in plant cell membranes is analyzed. The mechanisms of transformation of primary phytochrome signal into biological effects were discussed.

  10. Transformation of mercury speciation through the SCR system in power plants

    Institute of Scientific and Technical Information of China (English)

    YANG Hong-min; PAN Wei-ping

    2007-01-01

    Coal-fired utility boilers are now identified as the largest source of mercury in the United States. There is speculation that the installation of selective catalytic reduction (SCR) system for reduction of NOx can also prompt the oxidation and removal of mercury.In this paper, tests at six full-scale power plants with similar type of the SCR systems are conducted to investigate the effect of the SCR on the transformation of mercury speciation. The results show that the SCR system can achieve more than 70%-80% oxidation of elemental mercury and enhance the mercury removal ability in these units. The oxidation of elemental mercury in the SCR system strongly depends on the coal properties and the operation conditions of the SCR systems. The content of chloride in the coal is the key factor for the oxidization process and the maximum oxidation of elemental mercury is found when chloride content changes from 400 to 600 ppm. The sulfur content is no significant impact on oxidation of elemental mercury.

  11. Transformation of mercury speciation through the SCR system in power plants.

    Science.gov (United States)

    Yang, Hong-min; Pan, Wei-ping

    2007-01-01

    Coal-fired utility boilers are now identified as the largest source of mercury in the United States. There is speculation that the installation of selective catalytic reduction (SCR) system for reduction of NOx can also prompt the oxidation and removal of mercury. In this paper, tests at six full-scale power plants with similar type of the SCR systems are conducted to investigate the effect of the SCR on the transformation of mercury speciation. The results show that the SCR system can achieve more than 70%-80% oxidation of elemental mercury and enhance the mercury removal ability in these units. The oxidation of elemental mercury in the SCR system strongly depends on the coal properties and the operation conditions of the SCR systems. The content of chloride in the coal is the key factor for the oxidization process and the maximum oxidation of elemental mercury is found when chloride content changes from 400 to 600 ppm. The sulfur content is no significant impact on oxidation of elemental mercury.

  12. Potential ecosystem service delivery by endemic plants in New Zealand vineyards: successes and prospects

    Directory of Open Access Journals (Sweden)

    Morgan W. Shields

    2016-06-01

    Full Text Available Vineyards worldwide occupy over 7 million hectares and are typically virtual monocultures, with high and costly inputs of water and agro-chemicals. Understanding and enhancing ecosystem services can reduce inputs and their costs and help satisfy market demands for evidence of more sustainable practices. In this New Zealand work, low-growing, endemic plant species were evaluated for their potential benefits as Service Providing Units (SPUs or Ecosystem Service Providers (ESPs. The services provided were weed suppression, conservation of beneficial invertebrates, soil moisture retention and microbial activity. The potential Ecosystem Dis-services (EDS from the selected plant species by hosting the larvae of a key vine moth pest, the light-brown apple moth (Epiphyas postvittana, was also quantified. Questionnaires were used to evaluate winegrowers’ perceptions of the value of and problems associated with such endemic plant species in their vineyards. Growth and survival rates of the 14 plant species, in eight families, were evaluated, with Leptinella dioica (Asteraceae and Acaena inermis ‘purpurea’ (Rosaceae having the highest growth rates in terms of area covered and the highest survival rate after 12 months. All 14 plant species suppressed weeds, with Leptinella squalida, Geranium sessiliforum (Geraniaceae, Hebe chathamica (Plantaginaceae, Scleranthus uniflorus (Caryophyllaceae and L. dioica, each reducing weed cover by >95%. Plant species also differed in the diversity of arthropods that they supported, with the Shannon Wiener diversity index (H′ for these taxa ranging from 0 to 1.3. G. sessiliforum and Muehlenbeckia axillaris (Polygonaceae had the highest invertebrate diversity. Density of spiders was correlated with arthropod diversity and G. sessiliflorum and H. chathamica had the highest densities of these arthropods. Several plant species associated with higher soil moisture content than in control plots. The best performing species

  13. Environmental risk assessment for plant pests: a procedure to evaluate their impacts on ecosystem services

    DEFF Research Database (Denmark)

    Gilioli, G.; Schrader, G.; Baker, R.H.A.

    2014-01-01

    The current methods to assess the environmental impacts of plant pests differ in their approaches and there is a lack of the standardized procedures necessary to provide accurate and consistent results, demonstrating the complexity of developing a commonly accepted scheme for this purpose....... By including both the structural and functional components of the environment threatened by invasive alien species (IAS), in particular plant pests, we propose an environmental risk assessment scheme that addresses this complexity. Structural components are investigated by evaluating the impacts of the plant...... pest on genetic, species and landscape diversity. Functional components are evaluated by estimating how plant pests modify ecosystem services in order to determine the extent to which an IAS changes the functional traits that influence ecosystem services. A scenario study at a defined spatial...

  14. How plant functional traits cascade to microbial function and ecosystem services in mountain grasslands

    Science.gov (United States)

    Lavorel, S.; Grigulis, K.; Krainer, U.; Legay, N.; Turner, C.; Dumont, M.; Kastl, E.; Arnoldi, C.; Bardgett, R.; Poly, F.; Pommier, T.; Schloter, M.; Tappeiner, U.; Bahn, M.; Clément, J.-C.

    2012-04-01

    1. There is growing evidence that plant functional diversity and microbial communities of soil are tightly coupled, and that this coupling influences a range of ecosystem functions. Moreover, it has been hypothesized that changes in the nature of interactions between plant functional diversity and microbial communities along environmental gradients contributes to variation in the delivery of ecosystem services. Although there is empirical support for such relationships using broad plant and microbial functional classifications, or from studies of plant monocultures, such relationships and their consequences for ecosystem services have not been quantified under complex field conditions with diverse plant communities. 2. We aimed to provide an explicit quantification of how plant and microbial functional properties interplay to determine key ecosystem functions underlying ecosystem services provided by grasslands. At three mountain grassland sites in the French Alps, Austrian Tyrol and northern England, we quantified, along gradients of management intensity, (i) plant functional diversity, (ii) soil microbial community composition and parameters associated with nitrogen cycling, and (iii) key ecosystem processes related to the carbon and nitrogen cycles including aboveground biomass production, standing litter, litter decomposition, soil organic matter and nitrate and ammonium leaching . Considering that plants strongly determine microbial communities, we used a hierarchical approach that considered first direct effects of plant traits and then effects of soil microorganisms on processes, to determine the relative effects of plant and microbial functional parameters on key ecosystem properties. 3. We identified a gradient of relative effects of plant and microbial traits from properties controlled mostly by aboveground processes, such as plant biomass production and standing litter, to properties controlled mostly by microbial processes, such as soil leaching of

  15. Genetic transformation of the plant pathogens Phytophthora capsici and Phytophthora parasitica.

    OpenAIRE

    Bailey, A. M.; Mena, G L; Herrera-Estrella, L

    1991-01-01

    Phytophthora capsici and P.parasitica were transformed to hygromycin B resistance using plasmids pCM54 and pHL1, which contain the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the Ustilago maydis heat shock hsp70 gene. Enzymes Driselase and Novozyme 234 were used to generate protoplasts which were then transformed following exposure to plasmid DNA and polyethylene glycol 6000. Transformation frequencies of over 500 transformants per micrograms of DNA per ...

  16. Possible consequences of the overlap between the CaMV 35S promoter regions in plant transformation vectors used and the viral gene VI in transgenic plants.

    Science.gov (United States)

    Podevin, Nancy; du Jardin, Patrick

    2012-01-01

    Multiple variants of the Cauliflower mosaic virus 35S promoter (P35S) are used to drive the expression of transgenes in genetically modified plants, for both research purposes and commercial applications. The genetic organization of the densely packed genome of this virus results in sequence overlap between P35S and viral gene VI, encoding the multifunctional P6 protein. The present paper investigates whether introduction of P35S variants by genetic transformation is likely to result in the expression of functional domains of the P6 protein and in potential impacts in transgenic plants. A bioinformatic analysis was performed to assess the safety for human and animal health of putative translation products of gene VI overlapping P35S. No relevant similarity was identified between the putative peptides and known allergens and toxins, using different databases. From a literature study it became clear that long variants of the P35S do contain an open reading frame, when expressed, might result in unintended phenotypic changes. A flowchart is proposed to evaluate possible unintended effects in plant transformants, based on the DNA sequence actually introduced and on the plant phenotype, taking into account the known effects of ectopically expressed P6 domains in model plants.

  17. Novel recombinant binary vectors harbouring Basta (bar) gene as a plant selectable marker for genetic transformation of plants.

    Science.gov (United States)

    Nada, Reham M

    2016-04-01

    Genetic transformation is one of the most widely used technique in crop improvement. However, most of the binary vectors used in this technique, especially cloning based, contain antibiotic genes as selection marker that raise serious consumer and environmental concerns; moreover, they could be transferred to non-target hosts with deleterious effects. Therefore, the goal of this study was reconstruction of the widely used pBI121 binary vector by substituting the harmful antibiotic selection marker gene with a less-harmful selection marker, Basta (herbicide resistance gene). The generated vectors were designated as pBI121NB and pBI121CB, in which Basta gene was expressed under the control of Nos or CaMV 35S promoter, respectively. The successful integration of the new inserts into both the vectors was confirmed by PCR, restriction digestion and sequencing. Both these vectors were used in transforming Arabidopsis, Egyptian wheat and barley varieties using LBA4404 and GV3101 Agrobacterium strains. The surfactant Tween-20 resulted in an efficient transformation and the number of Arabidopsis transformants was about 6-9 %. Soaked seeds of wheat and barley were transformed with Agrobacterium to introduce the bacteria to the growing shoot apices. The percentage of transgenic lines was around 16-17 and 14-15 % for wheat and barley, respectively. The quantitative studies presented in this work showed that both LBA4404 and GV3101 strains were suitable for transforming Egyptian wheat and barley.

  18. Ecosystem services and plant physiological status during endophyte-assisted phytoremediation of metal contaminated soil.

    Science.gov (United States)

    Burges, Aritz; Epelde, Lur; Blanco, Fernando; Becerril, José M; Garbisu, Carlos

    2017-04-15

    Mining sites shelter a characteristic biodiversity with large potential for the phytoremediation of metal contaminated soils. Endophytic plant growth-promoting bacteria were isolated from two metal-(hyper)accumulator plant species growing in a metal contaminated mine soil. After characterizing their plant growth-promoting traits, consortia of putative endophytes were used to carry out an endophyte-assisted phytoextraction experiment using Noccaea caerulescens and Rumex acetosa (singly and in combination) under controlled conditions. We evaluated the influence of endophyte-inoculated plants on soil physicochemical and microbial properties, as well as plant physiological parameters and metal concentrations. Data interpretation through the grouping of soil properties within a set of ecosystem services was also carried out. When grown together, we observed a 41 and 16% increase in the growth of N. caerulescens and R. acetosa plants, respectively, as well as higher values of Zn phytoextraction and soil microbial biomass and functional diversity. Inoculation of the consortia of putative endophytes did not lead to higher values of plant metal uptake, but it improved the plants' physiological status, by increasing the content of chlorophylls and carotenoids by up to 28 and 36%, respectively, indicating a reduction in the stress level of plants. Endophyte-inoculation also stimulated soil microbial communities: higher values of acid phosphatase activity (related to the phosphate solubilising traits of the endophytes), bacterial and fungal abundance, and structural diversity. The positive effects of plant growth and endophyte inoculation on soil properties were reflected in an enhancement of some ecosystem services (biodiversity, nutrient cycling, water flow regulation, water purification and contamination control).

  19. 氯碱厂节水技术改造%Water saving technology transformation of chlor-alkali plant

    Institute of Scientific and Technical Information of China (English)

    董雷; 刘勇先

    2012-01-01

    介绍了氯碱厂一次盐水、电解、氯气处理、氢气处理工序的节水改造情况。通过技术改造,回收了大量的冷凝水及废水,减少了酸性水对厂房、设备的腐蚀。%The water saving technology transformation of chlor-alkali plant was introduced,including the brine process,electrolysis process and chlorine and hydrogen treatment.After technology transformation,the waste water was recycled,and reduced the corrosion of acidic water for the plant and equipment.The economic and social benefits were better.

  20. Analysis Of Provided Service Quality In Flowers And Living Plants Transport

    Science.gov (United States)

    Poliaková, Adela

    2015-06-01

    This paper analyses quality of the living flowers and plants transportation. A part of this paper compares the demands for a particular transport mode and a practical demonstration of these transport modes within a specific transport mode. The paper presents a survey of quality of services provided by individual transport modes.

  1. Provision of enhanced ancillary services from wind power plants - Examples and challenges

    DEFF Research Database (Denmark)

    Hansen, Anca Daniela; Altin, Müfit; Iov, Florin

    2016-01-01

    Emphasis in this article is on the power system impact of wind power plants capability to provide enhanced ancillary services, i.e. temporary frequency response (TFR) and power oscillation damping (POD). The main objective of the article is to analyze and justify the challenges in the use of TFR ...

  2. Generation of marker- and/or backbone-free transgenic wheat plants via Agrobacterium-mediated transformation

    Directory of Open Access Journals (Sweden)

    Wang Genping

    2016-09-01

    Full Text Available Horizontal transfer of antibiotic resistance genes to animals and vertical transfer of herbicide resistance genes to the weedy relatives are perceived as major biosafety concerns in genetically modified (GM crops. In this study, five novel vectors which used gusA and bar as a reporter gene and a selection marker gene, respectively, were constructed based on the pCLEAN dual binary vector system. Among these vectors, 1G7B and 5G7B carried two T-DNAs located on two respective plasmids with 5G7B possessing an additional virGwt gene. 5LBTG154 and 5TGTB154 carried two T-DNAs in the target plasmid with either one or double right borders, and 5BTG154 carried the selectable marker gene on the backbone outside of the T-DNA left border in the target plasmid. In addition, 5BTG154, 5LBTG154 and 5TGTB154 used pAL154 as a helper plasmid which contains Komari fragment to facilitate transformation. These five dual binary vector combinations were transformed into Agrobacterium strain AGL1 and used to transform durum wheat cv Stewart 63. Evaluation of the co-transformation efficiencies, the frequencies of marker-free transgenic plants and integration of backbone sequences in the obtained transgenic lines indicated that two vectors (5G7B and 5TGTB154 were more efficient in generating marker-free transgenic wheat plants with no or minimal integration of backbone sequences in the wheat genome. The vector series developed in this study for generation of marker- and/or backbone-free transgenic wheat plants via Agrobacterium-mediated transformation will be useful to facilitate the creation of ‘clean’ GM wheat containing only the foreign genes of agronomic importance.

  3. Generation of Marker- and/or Backbone-Free Transgenic Wheat Plants via Agrobacterium-Mediated Transformation

    Science.gov (United States)

    Wang, Gen-Ping; Yu, Xiu-Dao; Sun, Yong-Wei; Jones, Huw D.; Xia, Lan-Qin

    2016-01-01

    Horizontal transfer of antibiotic resistance genes to animals and vertical transfer of herbicide resistance genes to the weedy relatives are perceived as major biosafety concerns in genetically modified (GM) crops. In this study, five novel vectors which used gusA and bar as a reporter gene and a selection marker gene, respectively, were constructed based on the pCLEAN dual binary vector system. Among these vectors, 1G7B and 5G7B carried two T-DNAs located on two respective plasmids with 5G7B possessing an additional virGwt gene. 5LBTG154 and 5TGTB154 carried two T-DNAs in the target plasmid with either one or double right borders, and 5BTG154 carried the selectable marker gene on the backbone outside of the T-DNA left border in the target plasmid. In addition, 5BTG154, 5LBTG154, and 5TGTB154 used pAL154 as a helper plasmid which contains Komari fragment to facilitate transformation. These five dual binary vector combinations were transformed into Agrobacterium strain AGL1 and used to transform durum wheat cv Stewart 63. Evaluation of the co-transformation efficiencies, the frequencies of marker-free transgenic plants, and integration of backbone sequences in the obtained transgenic lines indicated that two vectors (5G7B and 5TGTB154) were more efficient in generating marker-free transgenic wheat plants with no or minimal integration of backbone sequences in the wheat genome. The vector series developed in this study for generation of marker- and/or backbone-free transgenic wheat plants via Agrobacterium-mediated transformation will be useful to facilitate the creation of “clean” GM wheat containing only the foreign genes of agronomic importance. PMID:27708648

  4. Economic valuation of plant diversity storage service provided by Brazilian rupestrian grassland ecosystems.

    Science.gov (United States)

    Resende, F M; Fernandes, G W; Coelho, M S

    2013-11-01

    The rupestrian grassland ecosystems provide various goods and services to society and support a significant amount of biological diversity. Notably the rich plant diversity has high levels of endemism and a variety of uses among the local communities and general society. Despite the socio-ecological importance of these ecosystems, they are subjected to significant anthropogenic pressures. The goal of this study is to perform economic valuation of the plant diversity storage service provided by rupestrian grassland ecosystems to provide grounds for the development of conservation policies and encourage sustainable practices in these ecosystems. Given the intense human disturbances and unique flora, the Serra do Cipó (southern portion of the Espinhaço Range in southeast Brazil) was selected for the study. We estimate the monetary value related to the plant diversity storage service provided by the study area using the maintenance costs of native plants in the living collections of the botanical garden managed by the Zoobotanical Foundation - Belo Horizonte (located 97 km from Serra do Cipó). The plant diversity storage value provided by Serra do Cipó ecosystems is significant, reaching US$25.26 million year-1. This study contributes to the development of perspectives related to the conservation of rupestrian grassland ecosystems as well as others threatened tropical ecosystems with high biodiversity.

  5. Agrobacterium tumefaciens-mediated transformation of embryogenic tissue and transgenic plant regeneration in Chamaecyparis obtusa Sieb. et Zucc.

    Science.gov (United States)

    Taniguchi, T; Kurita, M; Ohmiya, Y; Kondo, T

    2005-03-01

    A genetic transformation procedure for Chamaecyparis obtusa was developed after co-cultivation of embryogenic tissues with disarmed Agrobacterium tumefaciens strain C58/pMP90, which harbours the sgfp (synthetic green fluorescent protein) visual reporter and nptII (neomycin phoshotransferase II) selectable marker genes. The highest transformation frequency was 22.5 independent transformed lines per dish (250 mg embryogenic tissue) following selection on kanamycin medium. Transgenic plantlets were regenerated through the maturation and germination of somatic embryos. The intensity of GFP fluorescence, observed under a fluorescence microscope, varied from very faint to relatively strong, depending on the transgenic line or part of the transgenic plant. The integration of the genes into the genome of regenerated plantlets was confirmed by Southern blot analysis.

  6. Plant Regeneration from Immature Inflorescence Culture and Genetic Transformation of Wheatgrass (Agropyron cristatum × A. Desertorum cv. Hycrest-Mengnong)

    Institute of Scientific and Technical Information of China (English)

    HUO Xiu-wen; WEI Jian-hua; XU Chun-bo; MI Fu-gui; YUN Jin-feng

    2006-01-01

    The plants of hybrid wheatgrass (A. Cristatum ×A. Desertorum cv. Hycrest-Mengnong) were directly induced from embryogenic callus regenerated from immature inflorescence. Immature inflorescence was cultured on improved MS medium containing 2.0-3.0 mg L-1 2,4-D to regenerate callus. The calli were then transferred to hormone-free MS medium for differentiation and 1/2 MS medium for rooting. Results showed that callus initiation frequency was 83.4% and plant regeneration frequency was 59.6%. Phosphinothricin acetyltransferase (bar) gene was transformed into the hybrid wheatgrass by particle bombardment. Resistant callus was obtained using selecting agent, herbicide glufosinate of 0.5 mg L-1, and some transgenic plants were recovered in vitro. The transgenic plants were identified by PCR and Southern blot analysis and these plants developed normally in the glufosinate medium, whereas the nontransgenic plants did not. The results demonstrated that bar cDNA integrated into the genomic DNA of the transgenic plants. The transgenic frequencies of bar gene were 1.1%.

  7. High production of plant type levan in sugar beet transformed with timothy (Phleum pratense) 6-SFT genes.

    Science.gov (United States)

    Matsuhira, Hiroaki; Tamura, Ken-ichi; Tamagake, Hideto; Sato, Yutaka; Anzai, Hiroyuki; Yoshida, Midori

    2014-12-20

    Levan, a type of fructan, is an oligomer or polymer with mainly a β(2,6)-linked fructose chain attached to sucrose. We introduced two timothy genes, PpFT1 and PpFT2, coding for two homologous sucrose:fructan 6-fructosyltransferases into sugar beet. Sugar beet produces a high concentration of sucrose, a starting substrate in fructan synthesis, in the root. Among transgenic T1 lines, we obtained sugar beet transformants that accumulated large amounts of β(2,6)-linked levans (about 20 to 75mgg(-1) FW) in the roots. The transformed sugar beet plants possessing PpFT1 or PpFT2 produced linear levans with different degrees of polymerization (DP). Namely, the PpFT1 transformants accumulated mainly high DP levans including those with DP>40, while the PpFT2 transformants accumulated levans with DP between 3 and 40. Chromatograms showed that PpFT2 produces pure β(2,6)-linked linear levans compared with fructans synthesized by PpFT1. These levans belong to the high DP class of plant fructans, but have much shorter DP than that of levans generally produced by microorganisms.

  8. Partners in Learning: Exploring Two Transformative University and High School Service-Learning Partnerships

    Science.gov (United States)

    Bialka, Christa S.; Havlik, Stacey A.

    2016-01-01

    This study describes a service-learning partnership between a Mid-Atlantic university and two private, urban high schools by examining the perspectives of those engaged in the service experience. The purpose of this study was to explore the shared experiences of service-learning in schools from the perspectives of both university and high school…

  9. The Transforming Self: Service Narratives and Identity Change in Emerging Adulthood

    Science.gov (United States)

    Cox, Keith; McAdams, Dan P.

    2012-01-01

    The study examined the extent to which a service trip to Nicaragua affected college students' narrative understanding of themselves and their commitment to volunteer service. College students who went on a spring break service trip to work with poor citizens of Nicaragua wrote narrative accounts of three important experiences on the trip shortly…

  10. In planta Transformed Cumin (Cuminum cyminum L. Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance.

    Directory of Open Access Journals (Sweden)

    Sonika Pandey

    Full Text Available Cumin is an annual, herbaceous, medicinal, aromatic, spice glycophyte that contains diverse applications as a food and flavoring additive, and therapeutic agents. An efficient, less time consuming, Agrobacterium-mediated, a tissue culture-independent in planta genetic transformation method was established for the first time using cumin seeds. The SbNHX1 gene, cloned from an extreme halophyte Salicornia brachiata was transformed in cumin using optimized in planta transformation method. The SbNHX1 gene encodes a vacuolar Na+/H+ antiporter and is involved in the compartmentalization of excess Na+ ions into the vacuole and maintenance of ion homeostasis Transgenic cumin plants were confirmed by PCR using gene (SbNHX1, uidA and hptII specific primers. The single gene integration event and overexpression of the gene were confirmed by Southern hybridization and competitive RT-PCR, respectively. Transgenic lines L3 and L13 showed high expression of the SbNHX1 gene compared to L6 whereas moderate expression was detected in L5 and L10 transgenic lines. Transgenic lines (L3, L5, L10 and L13, overexpressing the SbNHX1 gene, showed higher photosynthetic pigments (chlorophyll a, b and carotenoid, and lower electrolytic leakage, lipid peroxidation (MDA content and proline content as compared to wild type plants under salinity stress. Though transgenic lines were also affected by salinity stress but performed better compared to WT plants. The ectopic expression of the SbNHX1 gene confirmed enhanced salinity stress tolerance in cumin as compared to wild type plants under stress condition. The present study is the first report of engineering salt tolerance in cumin, so far and the plant may be utilized for the cultivation in saline areas.

  11. In planta Transformed Cumin (Cuminum cyminum L.) Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance.

    Science.gov (United States)

    Pandey, Sonika; Patel, Manish Kumar; Mishra, Avinash; Jha, Bhavanath

    2016-01-01

    Cumin is an annual, herbaceous, medicinal, aromatic, spice glycophyte that contains diverse applications as a food and flavoring additive, and therapeutic agents. An efficient, less time consuming, Agrobacterium-mediated, a tissue culture-independent in planta genetic transformation method was established for the first time using cumin seeds. The SbNHX1 gene, cloned from an extreme halophyte Salicornia brachiata was transformed in cumin using optimized in planta transformation method. The SbNHX1 gene encodes a vacuolar Na+/H+ antiporter and is involved in the compartmentalization of excess Na+ ions into the vacuole and maintenance of ion homeostasis Transgenic cumin plants were confirmed by PCR using gene (SbNHX1, uidA and hptII) specific primers. The single gene integration event and overexpression of the gene were confirmed by Southern hybridization and competitive RT-PCR, respectively. Transgenic lines L3 and L13 showed high expression of the SbNHX1 gene compared to L6 whereas moderate expression was detected in L5 and L10 transgenic lines. Transgenic lines (L3, L5, L10 and L13), overexpressing the SbNHX1 gene, showed higher photosynthetic pigments (chlorophyll a, b and carotenoid), and lower electrolytic leakage, lipid peroxidation (MDA content) and proline content as compared to wild type plants under salinity stress. Though transgenic lines were also affected by salinity stress but performed better compared to WT plants. The ectopic expression of the SbNHX1 gene confirmed enhanced salinity stress tolerance in cumin as compared to wild type plants under stress condition. The present study is the first report of engineering salt tolerance in cumin, so far and the plant may be utilized for the cultivation in saline areas.

  12. Im"plant"ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation.

    Science.gov (United States)

    Kajiura, Hiroyuki; Fujiyama, Kazuhito

    2015-01-01

    Enzymatic activity assay of exogenous glycosyltransferase (GT) and glycosylhydrolase (GH) expressed in plants is an important analysis for determination of the expression of the gene of interest. However, generations and establishment of in planta transgenic lines are time-consuming. Furthermore, the expression levels and the activities of the exogenous GTs and GHs are quite low and weak, the radiolabeled donor substrate had to be used to analyze the enzymatic activity. Here, we describe a protocol for the generation of transgenic plants using suspension-cultured cells and a high sensitive assay for GT, especially β1,4-galactosyltransferase, using microsomal fraction from plant cells and fluorescent-labeled sugar chains as an acceptor substrate. This method enables less-time-consuming preparation of stable transgenic plants, non-radiolabeled, high-throughput detail analysis which includes mass spectrometric analysis and exo-glycosidase digestions.

  13. A Poisson-Fault Model for Testing Power Transformers in Service

    Directory of Open Access Journals (Sweden)

    Dengfu Zhao

    2014-01-01

    Full Text Available This paper presents a method for assessing the instant failure rate of a power transformer under different working conditions. The method can be applied to a dataset of a power transformer under periodic inspections and maintenance. We use a Poisson-fault model to describe failures of a power transformer. When investigating a Bayes estimate of the instant failure rate under the model, we find that complexities of a classical method and a Monte Carlo simulation are unacceptable. Through establishing a new filtered estimate of Poisson process observations, we propose a quick algorithm of the Bayes estimate of the instant failure rate. The proposed algorithm is tested by simulation datasets of a power transformer. For these datasets, the proposed estimators of parameters of the model have better performance than other estimators. The simulation results reveal the suggested algorithms are quickest among three candidates.

  14. Agrobacterium-Mediated Transformation of Embryogenic Calli of Anliucheng and Regeneration of Plants Containing the Chimaeric Ribonuclease Gene

    Institute of Scientific and Technical Information of China (English)

    LI Dong-dong; SHI Wei; DENG Xiu-xin

    2003-01-01

    Anliucheng (Citrus sinensis Osbeck), a very seedy and widely spread acidless sweet orange cultivar in south of China, was transformed by the strain of Agrobacterium Tumefaciens EHA105 carrying pTA29-barnase gene, which will induce pollen sterility in transgenic plants. The embryogenic calli of Anliucheng were co-cultivated with Agrobacterium tumefaciens for 3 days, and then transferred to selective medium containing 50 mg L-1 basta (a kind of herbicide) for 5 weeks. The resistant calli were recovered and regenerated 118 embryoids. A total of 13 entire plants were obtained after micro-grafted on trifoliate orange. These regenerated plants were verified by PCR amplification and confirmed by PCR-Southern blotting analysis.

  15. FPGA-Based Smart Sensor for Drought Stress Detection in Tomato Plants Using Novel Physiological Variables and Discrete Wavelet Transform

    Directory of Open Access Journals (Sweden)

    Carlos Duarte-Galvan

    2014-10-01

    Full Text Available Soil drought represents one of the most dangerous stresses for plants. It impacts the yield and quality of crops, and if it remains undetected for a long time, the entire crop could be lost. However, for some plants a certain amount of drought stress improves specific characteristics. In such cases, a device capable of detecting and quantifying the impact of drought stress in plants is desirable. This article focuses on testing if the monitoring of physiological process through a gas exchange methodology provides enough information to detect drought stress conditions in plants. The experiment consists of using a set of smart sensors based on Field Programmable Gate Arrays (FPGAs to monitor a group of plants under controlled drought conditions. The main objective was to use different digital signal processing techniques such as the Discrete Wavelet Transform (DWT to explore the response of plant physiological processes to drought. Also, an index-based methodology was utilized to compensate the spatial variation inside the greenhouse. As a result, differences between treatments were determined to be independent of climate variations inside the greenhouse. Finally, after using the DWT as digital filter, results demonstrated that the proposed system is capable to reject high frequency noise and to detect drought conditions.

  16. FPGA-based smart sensor for drought stress detection in tomato plants using novel physiological variables and discrete wavelet transform.

    Science.gov (United States)

    Duarte-Galvan, Carlos; Romero-Troncoso, Rene de J; Torres-Pacheco, Irineo; Guevara-Gonzalez, Ramon G; Fernandez-Jaramillo, Arturo A; Contreras-Medina, Luis M; Carrillo-Serrano, Roberto V; Millan-Almaraz, Jesus R

    2014-10-09

    Soil drought represents one of the most dangerous stresses for plants. It impacts the yield and quality of crops, and if it remains undetected for a long time, the entire crop could be lost. However, for some plants a certain amount of drought stress improves specific characteristics. In such cases, a device capable of detecting and quantifying the impact of drought stress in plants is desirable. This article focuses on testing if the monitoring of physiological process through a gas exchange methodology provides enough information to detect drought stress conditions in plants. The experiment consists of using a set of smart sensors based on Field Programmable Gate Arrays (FPGAs) to monitor a group of plants under controlled drought conditions. The main objective was to use different digital signal processing techniques such as the Discrete Wavelet Transform (DWT) to explore the response of plant physiological processes to drought. Also, an index-based methodology was utilized to compensate the spatial variation inside the greenhouse. As a result, differences between treatments were determined to be independent of climate variations inside the greenhouse. Finally, after using the DWT as digital filter, results demonstrated that the proposed system is capable to reject high frequency noise and to detect drought conditions.

  17. Transforming mental health services: a participatory mixed methods study to promote and evaluate the implementation of recovery-oriented services.

    Science.gov (United States)

    Park, Melissa M; Zafran, Hiba; Stewart, Janet; Salsberg, Jon; Ells, Carolyn; Rouleau, Suzanne; Estein, Orly; Valente, Thomas W

    2014-09-10

    Since 2007, the Mental Health Commission of Canada has worked collaboratively across all provinces to publish a framework and strategy for recovery and well-being. This federal document is now mandated as policy for implementation between 2012 and 2017. The proposed strategies have been written into provincial health plans, hospital accreditation standards, and annual objectives of psychiatric departments and community organizations. The core premise is: to empower persons with mental illness and their families to become participants in designing their own care, while meeting the needs of a diverse Canadian population. However, recovery principles do not come with an implementation guide to fit the variability of different local contexts. How can policy recommendations and accreditation standards be effectively tailored to support a diversity of stakeholder values? To our knowledge, there is little evidence indicating the most effective manner to accelerate the uptake of recovery-oriented services among providers in a given/particular mental health treatment setting. This three-year Canadian Institute of Health Research Partnership in Health System Improvement and The Rx&D Health Research Foundation (HRF) Fostering Canadian Innovation in Research study (2013 to 2017) proposed participatory approaches to implementing recovery principles in a Department of Psychiatry serving a highly diverse Canadian and immigrant population. This project will be conducted in overlapping and recursive phases: I) Conduct formative research to (a) measure the current knowledge and attitudes toward recovery and recovery-oriented practices among service providers, while concurrently (b) exploring the experiential knowledge of recovery service-users and family members; II) Collaborate with service-users and the network-identified opinion leaders among providers to tailor Recovery-in-Action Initiatives to fit the needs and resources of a Department of Psychiatry; and III) Conduct a

  18. 电信业务运营的数字化服务转型%Digitized Service Transformation of Telecom Service Operation

    Institute of Scientific and Technical Information of China (English)

    邵建; 童恩

    2015-01-01

    In recent years, both inner and outer environments of telecom industry have greatly changed. The market competition environment of domestic telecom operators becomes diversified and complex. Therefore, the deep transformation of telecom service has been strongly driven. In view of the situations, the digitalized service transformation of telecom operation was researched and analyzed in aspects of value connotation, terminal, network and service to provide a useful reference to the future development of telecom operation.%近年来电信行业的内外部环境发生了巨大的变化,国内电信运营商面临的市场竞争也变得多元化和复杂化,因而电信业务的深化转型有着强烈的驱动。基于此通过价值内涵、终端、网络和业务等层面对电信运营的数字化服务转型进行研究和分析,为电信运营的未来发展提供了相关参考。

  19. The Department of Veterans Health Administration Office of Nursing Service, "transforming nursing in a national healthcare system: an example of transformation in action".

    Science.gov (United States)

    Wertenberger, Sydney; Chapman, Kathleen M; Wright-Brown, Salena

    2011-01-01

    The Department of Veterans Health Administration Office of Nursing Service has embarked on a multiyear transformational process, an example of which is the development of an organization-wide nursing handbook. The development of this handbook offered the opportunity to improve collaboration, redefine expectations and behavior, as well as prepare for the future of Nursing within the Veterans Health Administration. The lessons learned from this process have revolved around the themes of leadership skills for managing high-level change often in a virtual environment; constant collaboration; that the practice of nursing will continue to evolve on the basis of new evidence, technology, customer expectations, and resources; and that the process to accomplish this goal is powerful.

  20. Evaluation of a Generic Virtual Power Plant Framework Using Service Oriented Architecture

    DEFF Research Database (Denmark)

    Andersen, Peter Bach; Poulsen, Bjarne; Decker, Morten

    2008-01-01

    interesting properties that can influence the future shape of power markets. The concept holds many promises including cheaper power to the consumer, a more flexible and responsive power production and the support of a more environment- friendly development. In order to realize a software solution supporting...... the generic virtual power plant, an array of different software design principles, patterns and architectures must be applied. Especially Service Oriented Architecture (SOA) can aid in implementing the generic virtual power plant. An analysis of the Nordic power market has been carried out in order...

  1. Constructs of analysis and assessment model of transformation restructuring in steel plants

    Directory of Open Access Journals (Sweden)

    B. Gajdzik

    2014-01-01

    Full Text Available The article presents the assumptions for construction of a transformation restructuring model which describes, in a complex way, the range of changes implemented in metallurgical enterprises in Poland in time of economical changes. The model was prepared on the basis of thorough research studies concerning restructuring in countries where transformation of the economy system took place, which is change from central planning economy into market economy. It was also based on scientific research and own experiences. Besides the presentation of the methodological assumptions of designing a model of transformation restructuring in metallurgical sector the article also shows the issue of transformation restructuring and the areas of implemented changes in particular ranges of activity in metallurgical enterprises, which allowed them to function in conditions of market economy.

  2. Genetic transformation of the plant pathogens Phytophthora capsici and Phytophthora parasitica

    National Research Council Canada - National Science Library

    Bailey, A M; Mena, G L; Herrera-Estrella, L

    1991-01-01

    ...) fused to promoter elements of the Ustilago maydis heat shock hsp70 gene. Enzymes Driselase and Novozyme 234 were used to generate protoplasts which were then transformed following exposure to plasmid DNA and polyethylene glycol 6000...

  3. SacB-SacR gene cassette as the negative selection marker to suppress Agrobacterium overgrowth in Agrobacterium-mediated plant transformation

    Directory of Open Access Journals (Sweden)

    Yiming Liu

    2016-10-01

    Full Text Available Agrobacterium overgrowth is a common problem in Agrobacterium-mediated plant transfor-mation. To suppress the Agrobacterium overgrowth, various antibiotics have been used during plant tissue culture steps. The antibiotics are expensive and may adversely affect plant cell differentiation and reduce plant transformation efficiency. The SacB-SacR proteins are toxic to most Agrobacterium tumefaciens strains when they are grown on culture medium sup¬plemented with sucrose. Therefore, SacB-SacR genes can be used as negative selection markers to suppress the overgrowth of Agrobacterium tumefaciens in the plant tissue culture process. We generated a mutant Agrobacterium tumefaciens strain GV2260 (recA-SacB/R that has the SacB-SacR cassette inserted into the bacterial genome at the recA gene locus. The mutant Agrobacterium strain is sensitive to sucrose but maintains its ability to transform plant cells in both transient and stable transformation assays. We demonstrated that the mutant strain GV2260 (recA-SacB/R can be inhibited by sucrose that reduces the overgrowth of Agrobacterium and therefore improves the plant transformation efficiency. We employed GV2260 (recA-SacB/R to generate stable transgenic N. benthamiana plants expressing a CRISPR-Cas9 for knocking out a WRKY transcrip¬tion factor.

  4. Reproduction of Meloidogyne javanica on Plant Roots Genetically Transformed by Agrobacterium rhizogenes

    OpenAIRE

    Verdejo, S.; Jaffee, B. A.; Mankau, R.

    1988-01-01

    Reproduction of Meloidogyne javanica was compared on several Agrobacterium rhizogenes-transformed root cultures under monoxenic conditions. M. javanica reproduced on all transformed roots tested; however, more females and eggs were obtained on potato and South Australian Early Dwarf Red tomato than on bindweed, Tropic tomato, lima bean, or carrot. Roots that grew at moderate rates into the agar and produced many secondary roots supported the highest reproduction. Numbers of females produced i...

  5. Recovery-Oriented Services--The Role of Training in Transformation

    Science.gov (United States)

    Nowak, Izabela; Switaj, Piotr; Anczewska, Marta

    2015-01-01

    Recovery oriented practice/service provision is how workers and services support people in their recovery journey. There are four identified practice domains: promoting citizenship, organizational commitment, supporting personally defined recovery, and working relationship. Professionals might be helpful if they are willing to be open, respectful…

  6. Blending Ameliorative and Transformative Approaches in Human Service Organizations: A Case Study

    Science.gov (United States)

    Evans, Scot D.; Hanlin, Carrie E.; Prilleltensky, Isaac

    2007-01-01

    This paper describes the challenges and benefits of an action-research project with a Nashville-based nonprofit human service organization. In our view, outmoded human service organizations are in serious need of innovation to promote psychological and physical wellness, prevention of social problems, empowerment, and social justice. This project…

  7. Analysis of propagation of Bacopa monnieri (L.) from hairy roots, elicitation and Bacoside A contents of Ri transformed plants.

    Science.gov (United States)

    Largia, Muthiah Joe Virgin; Satish, Lakkakula; Johnsi, Rajaiah; Shilpha, Jayabalan; Ramesh, Manikandan

    2016-08-01

    Agrobacterium rhizogenes mediated transformation has been experimented in leaf explants of the memory herb Bacopa monnieri in order to assess the regeneration potential of hairy roots (HR) followed by the elicitation of transformed plants for increased Bacoside A production. Out of the four strains tested, A4 and MTCC 532 derived HR exhibited regrowth in MS basal medium while MTCC 2364 derived HR showed regeneration in MS medium supplemented with suitable phyto hormones. R1000 derived HR possessed no regeneration potential. Comparable to A4, MTCC 532 derived HR displayed maximum regrowth frequency of about 85.71 ± 1.84 % with an increase in biomass to threefold. Therefore, five HR plant lines (MTCC 532 derived) were generated and maintained in MS basal liquid medium in which HR3 topped the others in producing a huge biomass of about 67.09 ± 0.66 g FW. PCR amplification and southern hybridization analysis of rol A gene (280 bp) has been performed in order to confirm the transformation process. Moreover, HR3 plant line has accumulated highest total phenolic content of about 165.68 ± 0.82 mg GAE/g DW and highest total flavonoid content of about 497.78 ± 0.57 mg QRE/g DW when compared to other lines and untransformed controls. In addition, HR3 plant extract showed 85.58 ± 0.14 % of DPPH (2, 2-diphenyl-1-picryl hydrazyl) inhibition displaying its reliable anti oxidant potential. Further on elicitation with 10 mg/L chitosan for 2 weeks, HR3 has produced 5.83 % of Bacoside A which is fivefold and threefold increased production when compared to untransformed and transformed unelicited controls respectively. This is the first report on eliciting HR plants for increased metabolite accumulation in B. monnieri.

  8. Demonstration of Essential Reliability Services by a 300-MW Solar Photovoltaic Power Plant

    Energy Technology Data Exchange (ETDEWEB)

    Loutan, Clyde; Klauer, Peter; Chowdhury, Sirajul; Hall, Stephen; Morjaria, Mahesh; Chadliev, Vladimir; Milam, Nick; Milan, Christopher; Gevorgian, Vahan

    2017-03-24

    The California Independent System Operator (CAISO), First Solar, and the National Renewable Energy Laboratory (NREL) conducted a demonstration project on a large utility-scale photovoltaic (PV) power plant in California to test its ability to provide essential ancillary services to the electric grid. With increasing shares of solar- and wind-generated energy on the electric grid, traditional generation resources equipped with automatic governor control (AGC) and automatic voltage regulation controls -- specifically, fossil thermal -- are being displaced. The deployment of utility-scale, grid-friendly PV power plants that incorporate advanced capabilities to support grid stability and reliability is essential for the large-scale integration of PV generation into the electric power grid, among other technical requirements. A typical PV power plant consists of multiple power electronic inverters and can contribute to grid stability and reliability through sophisticated 'grid-friendly' controls. In this way, PV power plants can be used to mitigate the impact of variability on the grid, a role typically reserved for conventional generators. In August 2016, testing was completed on First Solar's 300-MW PV power plant, and a large amount of test data was produced and analyzed that demonstrates the ability of PV power plants to use grid-friendly controls to provide essential reliability services. These data showed how the development of advanced power controls can enable PV to become a provider of a wide range of grid services, including spinning reserves, load following, voltage support, ramping, frequency response, variability smoothing, and frequency regulation to power quality. Specifically, the tests conducted included various forms of active power control such as AGC and frequency regulation; droop response; and reactive power, voltage, and power factor controls. This project demonstrated that advanced power electronics and solar generation can be

  9. Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR).

    Science.gov (United States)

    Badhan, Ajay; Wang, Yuxi; McAllister, Tim A

    2017-01-01

    Fourier transformed mid-infrared spectroscopy (FTIR) is a powerful tool for compositional analysis of plant cell walls (Acebes et al., Front Plant Sci 5:303, 2014; Badhan et al., Biotechnol Biofuels 7:1-15, 2014; Badhan et al., BioMed Res Int 2015: 562952, 2015; Roach et al., Plant Physiol 156:1351-1363, 2011). The infrared spectrum generates a fingerprint of a sample with absorption peaks corresponding to the frequency of vibrations between the bonds of the atoms making up the material. Here, we describe a method focused on the use of FTIR in combination with principal component analysis (PCA) to characterize the composition of the plant cell wall. This method has been successfully used to study complex enzyme saccharification processes like rumen digestion to identify recalcitrant moieties in low-quality forage which resist rumen digestion (Badhan et al., BioMed Res Int 2015: 562952, 2015), as well as to characterize cell wall mutant lines or transgenic lines expressing exogenous hydrolases (Badhan et al., Biotechnol Biofuels 7:1-15, 2014; Roach et al., Plant Physiol 156:1351-1363, 2011). The FTIR method described here facilitates high-throughput identification of the major compositional differences across a large set of samples in a low cost and nondestructive manner.

  10. An Agrobacterium tumefaciens Strain with Gamma-Aminobutyric Acid Transaminase Activity Shows an Enhanced Genetic Transformation Ability in Plants

    Science.gov (United States)

    Nonaka, Satoko; Someya, Tatsuhiko; Zhou, Sha; Takayama, Mariko; Nakamura, Kouji; Ezura, Hiroshi

    2017-01-01

    Agrobacterium tumefaciens has the unique ability to mediate inter-kingdom DNA transfer, and for this reason, it has been utilized for plant genetic engineering. To increase the transformation frequency in plant genetic engineering, we focused on gamma-aminobutyric acid (GABA), which is a negative factor in the Agrobacterium-plant interaction. Recent studies have shown contradictory results regarding the effects of GABA on vir gene expression, leading to the speculation that GABA inhibits T-DNA transfer. In this study, we examined the effect of GABA on T-DNA transfer using a tomato line with a low GABA content. Compared with the control, the T-DNA transfer frequency was increased in the low-GABA tomato line, indicating that GABA inhibits T-DNA transfer. Therefore, we bred a new A. tumefaciens strain with GABA transaminase activity and the ability to degrade GABA. The A. tumefaciens strain exhibited increased T-DNA transfer in two tomato cultivars and Erianthus arundinacues and an increased frequency of stable transformation in tomato. PMID:28220841

  11. Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum

    Directory of Open Access Journals (Sweden)

    Bipinchandra B. Kalbande

    2016-06-01

    Full Text Available A new method of transgenic development called “In-planta” transformation method, where Agrobacterium is used to infect the plantlets but the steps of in vitro regeneration of plants is totally avoided. In this study, we have reported a simple In-planta method for efficient transformation of diploid cotton Gossypium hirsutum cv LRK-516 Anjali using Agrobacterium tumefaciens EHA-105 harbouring recombinant binary vector plasmid pBinAR with Arabidopsis At-NPR1 gene. Four day old plantlets were used for transformation. A vertical cut was made at the junction of cotyledonary leaves, moderately bisecting the shoot tip and exposing meristem cells at apical meristem. This site was infected with Agrobacterium inoculum. The transgenic events obtained were tested positive for the presence of At-NPR1 gene with promoter nptII gene. They are also tested negative for vector backbone integration and Agrobacterium contamination in T0 events. With this method a transformation frequency of 6.89% was reported for the cv LRK-516.

  12. Organizational Effectiveness for Effective Transformation of Sub-Sahara Africa: An Empirical Review of Anambra State Civil Service, Nigeria

    Directory of Open Access Journals (Sweden)

    Clementina Uchenna Agbionu

    2015-07-01

    Full Text Available The public sector in Nigeria has suffered series of setbacks which were attributed to ineffective and inefficient management which immediately calls for transformation of the sector in order to ensure the effectiveness and efficiency in the performance of the sector for national development. Transformation for national development in Nigeria demands new tools to support the performance of the public sector. Organizational Effectiveness is a tool which describes the degree to which an organization realizes its goal. This paper intends to contribute its own quota to the transformation question by investigating the potential models and tools for understanding, explaining and measuring organizational effectiveness of the public sector organizations in Nigeria. To do this, the paper evaluated the immediate results of the work of NATO RTO HFM Task Group (TG 163 on “Improving Organizational Effectiveness of Coalition Operations which consists of researchers from eleven nations of the world. The paper used descriptive statistics and content analysis to analyze “Organizational effectiveness in the context of public organizations, and to categorize factors critical to organizational effectiveness. Based on the review of the models, the study developed a structured questionnaire which was used to collect the relevant data for the purpose of investigating the effect of the different factors that influence organizational effectiveness, expose inefficiencies in public organizations and determine measures to achieve better organizational effectiveness in Anambra State Civil Service. The findings revealed that the key areas of structure and processes, people and culture do not receive adequate attention in the Anambra State Civil Service. A major recommendation of the study is that the emphasis of the government should not be basically on the provision of resources but also on how the resources are effectively and efficiently managed especially during the

  13. The production of class III plant peroxidases in transgenic callus cultures transformed with the rolB gene of Agrobacterium rhizogenes.

    Science.gov (United States)

    Shkryl, Y N; Veremeichik, G N; Bulgakov, V P; Avramenko, T V; Günter, E A; Ovodov, Y S; Muzarok, T I; Zhuravlev, Y N

    2013-10-10

    The production of plant peroxidases by plant cell cultures is of great interest because of the potential for industrial applications. We used plant cell cultures overexpressing the rolB gene to produce increased amounts of plant class III peroxidases. The rolB gene ensured the stable and permanent activation of peroxidase activity in the transformed callus cultures of different plants. In particular, the total peroxidase activity in transformed Rubia cordifolia cells was increased 23-86-fold, and the abundance of the major peroxidase gene transcripts was increased 17-125-fold (depending on the level of rolB expression) compared with non-transformed control calli. The peroxidase-activating effect of rolB was greater than that of other peroxidase inducers, such as external stresses and methyl jasmonate. Copyright © 2013 Elsevier B.V. All rights reserved.

  14. A novel gateway-compatible binary vector series (PC-GW) for flexible cloning of multiple genes for genetic transformation of plants.

    Science.gov (United States)

    Dalal, Jyoti; Yalamanchili, Roopa; La Hovary, Christophe; Ji, Mikyoung; Rodriguez-Welsh, Maria; Aslett, Denise; Ganapathy, Sowmya; Grunden, Amy; Sederoff, Heike; Qu, Rongda

    2015-09-01

    The rapidly advancing field of plant synthetic biology requires transforming plants with multiple genes. This has sparked a growing interest in flexible plant transformation vectors, which can be used for multi-gene transformations. We have developed a novel binary vector series, named the PC-GW series (GenBank: KP826769-KP826773), for Agrobacterium-mediated plant transformation. The PC-GW vectors use the pCAMBIA vector backbone, and contain NPTII, hpt, bar, mCherry or egfp genes as selectable markers for plant transformation. In a modified multiple cloning site (MCS) of the T-DNA region, we have placed the attR1, attR2 and ccdB sequences for rapid cloning of one to four genes by Gateway™-assisted recombination. In addition, we have introduced four meganuclease sites, and other restriction sites for multi-gene vector construction. Finally, we have placed a CaMV 35S promoter and a 35S terminator on the 5' and 3' ends of the MCS. The CaMV 35S promoter is flanked by PstI restriction sites that can be used to replace it with another promoter sequence if needed. The PC-GW vectors provide choices for selectable markers, cloning methods, and can accommodate up to eight gene constructs in a single T-DNA, thereby significantly reducing the number of transformations or crosses needed to generate multi-transgene expressing plants.

  15. Transformational leadership in the consumer service workgroup: competing models of job satisfaction, change commitment, and cooperative conflict resolution.

    Science.gov (United States)

    Yang, Yi-Feng

    2014-02-01

    This paper discusses the effects of transformational leadership on cooperative conflict resolution (management) by evaluating several alternative models related to the mediating role of job satisfaction and change commitment. Samples of data from customer service personnel in Taiwan were analyzed. Based on the bootstrap sample technique, an empirical study was carried out to yield the best fitting model. The procedure of hierarchical nested model analysis was used, incorporating the methods of bootstrapping mediation, PRODCLIN2, and structural equation modeling (SEM) comparison. The analysis suggests that leadership that promotes integration (change commitment) and provides inspiration and motivation (job satisfaction), in the proper order, creates the means for cooperative conflict resolution.

  16. Transforming Dissatisfaction with Services into Self-Determination: A Social Psychological Perspective on Community Program Effectiveness.

    Science.gov (United States)

    Macias, Cathaleene; Aronson, Elliot; Hargreaves, William; Weary, Gifford; Barreira, Paul J; Harvey, John; Rodican, Charles F; Bickman, Leonard; Fisher, William

    2009-08-01

    A field study of supported employment for adults with mental illness (N=174) provided an experimental test of cognitive dissonance theory. We predicted that most work-interested individuals randomly assigned to a non-preferred program would reject services and lower their work aspirations. However, individuals who chose to pursue employment through a non-preferred program were expected to resolve this dissonance through favorable service evaluations and strong efforts to succeed at work. Significant work interest-by-service preference interactions supported these predictions. Over two years, participants interested in employment who obtained work through a non-preferred program stayed employed a median of 362 days versus 108 days for those assigned to a preferred program, and participants who obtained work through a non-preferred program had higher service satisfaction.

  17. Transformation of the limonene synthase gene into peppermint (Mentha piperita L.) and preliminary studies on the essential oil profiles of single transgenic plants.

    Science.gov (United States)

    Krasnyanski, S; May, R A; Loskutov, A; Ball, T M; Sink, K C

    1999-08-01

    Agrobacterium-mediated and direct gene transfer into protoplasts using PEG were both successfully used to produce stable, transformed peppermint plants (Mentha×piperita L. cultivar Black Mitcham) with the limonene synthase gene. Stem internode explants found to possess a high level of organogenesis through adventitious shoot formation were subjected to Agrobacterium tumefaciens disarmed strain GV3101 (pMP90). Following the development of an efficient protoplast-to-plant cycle from stem-isolated protoplasts, they were used in direct gene transformations. In both cases the binary vector pGA643 carrying the nptII/GUS genes, both driven by the CaMV35S promoter, was used in preliminary plant-transformation studies. Later, GUS was replaced with the limonene synthase gene. Kanamycin was used as a selective agent in all transformation experiments to obtain both transformed protoplast-derived calli as well as putative transgenic shoots regenerated from internode explants. Both types of transformation resulted in transgenic plants which were detected using PCR and confirmed by Southern-blot hybridizations. Southern analysis revealed that the method of Agrobacterium-mediated transformation is superior to the direct DNA uptake into protoplasts with regard to the stability of the insert during the transformation event. Single transgenic plants were grown to 10% flowering in a greenhouse and the plants derived both by the Agrobacterium and the protoplast-derived methods were generally observed to have essential oil profiles characterized by a high-menthone, low-menthol, high-menthofuran and -pulegone content in comparison to a typical mid-west peppermint. Limonene varied only slightly, around 1.2%, in transgenic plants produced by both methods.

  18. Coordinated system services from offshore wind power plants connected through HVDC networks

    DEFF Research Database (Denmark)

    Zeni, L.; Glasdam, Jakob Bærholm; Hesselbæk, B.

    2014-01-01

    This paper presents an overview of power system services in networks involving multiple onshore power systems, a voltage sourced converter (VSC) based high voltage direct current (HVDC) offshore network and an offshore wind power plant (OWPP). A comprehensive list of services regarding onshore...... control and communication delays of OWPPs, and their influence on the successful delivery of the targeted services. Furthermore, it is shown that as an HVDC network increases in size from the point-to-point, the handling of onshore short circuits calls for the proper combination of DC chopper(s) and fast...... as well as offshore network operation – both AC and DC – will be discussed from a state of the art perspective. Among them, the most interesting have been selected and will be treated in more detail and the main contribution of this paper will be to shed light on the most relevant aspects related...

  19. Ecosystem Services and Biodiversity in a Rapidly Transforming Landscape in Northern Borneo.

    Directory of Open Access Journals (Sweden)

    Nicolas Labrière

    Full Text Available Because industrial agriculture keeps expanding in Southeast Asia at the expense of natural forests and traditional swidden systems, comparing biodiversity and ecosystem services in the traditional forest-swidden agriculture system vs. monocultures is needed to guide decision making on land-use planning. Focusing on tree diversity, soil erosion control, and climate change mitigation through carbon storage, we surveyed vegetation and monitored soil loss in various land-use areas in a northern Bornean agricultural landscape shaped by swidden agriculture, rubber tapping, and logging, where various levels and types of disturbance have created a fine mosaic of vegetation from food crop fields to natural forest. Tree species diversity and ecosystem service production were highest in natural forests. Logged-over forests produced services similar to those of natural forests. Land uses related to the swidden agriculture system largely outperformed oil palm or rubber monocultures in terms of tree species diversity and service production. Natural and logged-over forests should be maintained or managed as integral parts of the swidden system, and landscape multifunctionality should be sustained. Because natural forests host a unique diversity of trees and produce high levels of ecosystem services, targeting carbon stock protection, e.g. through financial mechanisms such as Reducing Emissions from Deforestation and Forest Degradation (REDD+, will synergistically provide benefits for biodiversity and a wide range of other services. However, the way such mechanisms could benefit communities must be carefully evaluated to counter the high opportunity cost of conversion to monocultures that might generate greater income, but would be detrimental to the production of multiple ecosystem services.

  20. Ecosystem Services and Biodiversity in a Rapidly Transforming Landscape in Northern Borneo.

    Science.gov (United States)

    Labrière, Nicolas; Laumonier, Yves; Locatelli, Bruno; Vieilledent, Ghislain; Comptour, Marion

    2015-01-01

    Because industrial agriculture keeps expanding in Southeast Asia at the expense of natural forests and traditional swidden systems, comparing biodiversity and ecosystem services in the traditional forest-swidden agriculture system vs. monocultures is needed to guide decision making on land-use planning. Focusing on tree diversity, soil erosion control, and climate change mitigation through carbon storage, we surveyed vegetation and monitored soil loss in various land-use areas in a northern Bornean agricultural landscape shaped by swidden agriculture, rubber tapping, and logging, where various levels and types of disturbance have created a fine mosaic of vegetation from food crop fields to natural forest. Tree species diversity and ecosystem service production were highest in natural forests. Logged-over forests produced services similar to those of natural forests. Land uses related to the swidden agriculture system largely outperformed oil palm or rubber monocultures in terms of tree species diversity and service production. Natural and logged-over forests should be maintained or managed as integral parts of the swidden system, and landscape multifunctionality should be sustained. Because natural forests host a unique diversity of trees and produce high levels of ecosystem services, targeting carbon stock protection, e.g. through financial mechanisms such as Reducing Emissions from Deforestation and Forest Degradation (REDD+), will synergistically provide benefits for biodiversity and a wide range of other services. However, the way such mechanisms could benefit communities must be carefully evaluated to counter the high opportunity cost of conversion to monocultures that might generate greater income, but would be detrimental to the production of multiple ecosystem services.

  1. Monitoring systems online of oil for transformers of nuclear power plants; Sistemas de monitorizacion online del aceite para transformadores de potencia de Centrales

    Energy Technology Data Exchange (ETDEWEB)

    Sarandeses, S.

    2014-07-01

    The nuclear power plants are showing their concern due to the existence of recent failures related to the bulky transformers of power. These transformers are not security, but are important for the production of power as its failure can cause transient on the floor, reactor scram or shooting, that can cause interruptions in the production of energy or might force us to reduce the power of production The analysis of gases dissolved in transformer oil is recognized as a trial key to identify a submerged transformer failure in oil. With this analysis it is not possible to ensure that there is no damage in the transformer, but the probability of risk of this type of failure can be reduced. The industry recommended to equip the new large power transformers with oil online monitoring systems and in some cases also be It recommended its use in existing transformers. (Author)

  2. Evolution of Carbide Precipitates in 2.25Cr-1Mo Steel during Long-Term Service in a Power Plant

    Science.gov (United States)

    Yang, Yong; Chen, Yiren; Sridharan, Kumar; Allen, Todd R.

    2010-03-01

    Carbide precipitation from the steel matrix during long-term high-temperature exposure can adversely affect the fracture toughness and high-temperature creep resistance of materials with implications on the performance of power plant components. In the present work, carbide evolution in 2.25Cr-1Mo steel after long-term aging during service was investigated. Boiler pipe samples of this steel were removed from a supercritical water-cooled coal-fired power plant after service times of 17 and 28 years and a mean operational temperature of 810 K (537 °C). The carbide precipitation and coarsening effects were studied using the carbon extraction replica technique followed by analysis using transmission electron microscopy and energy dispersive X-ray spectroscopy. The carbides extracted using an electrolytic technique were also analyzed using X-ray diffraction to evaluate phase transformations of the carbides during long-term service. Small ball punch and Vickers hardness were used to evaluate the changes in mechanical performance after long-term aging during service.

  3. Metabolite profiling of Arabidopsis thaliana (L.) plants transformed with an antisense chalcone synthase gene

    DEFF Research Database (Denmark)

    Le Gall, G.; Metzdorff, Stine Broeng; Pedersen, Jan W.;

    2005-01-01

    A metabolite profiling study has been carried out on Arabidopsis thaliana (L.) Heynh. ecotype Wassilewskija and a series of transgenic lines of the ecotype transformed with a CHS (chalcone synthase) antisense construct. Compound identifications by LC/MS and H-1 NMR are discussed. The glucosinolate...

  4. Efficient production of transgenic plants by Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)

    NARCIS (Netherlands)

    Schreuder, M.M.; Raemakers, C.J.J.M.; Jacobsen, E.; Visser, R.G.F.

    2001-01-01

    An efficient and reproducible method was developed for Agrobacterium-mediated transformation of embryogenic suspension cultures of cassava. LBA4404(pTOK233), containing the nptII, hph and gus marker genes, was used in the experiments. Chemical selection by means of kanamycin was used to establish 10

  5. FOREST-BASED MEDICINAL PLANTS RENDERING THEIR SERVICES TO THE RURAL COMMUNITY OF ASSAM, INDIA

    Directory of Open Access Journals (Sweden)

    Ratul Arya Baishya

    2013-12-01

    Full Text Available Forests are the main biological resource areas from where reportedly 80% of the medicinal plants are collected by the rural communities of the state. Traditional folk medicines, mainly based on plants, occupy a significant position today, especially in the developing countries, where modern health care service is limited. Medicinal plants are gaining global importance owing to the fact that herbal drugs are cost-effective, easily available and most reportedly, with negligible side effects. Safe, effective and inexpensive indigenous remedies had been practiced by the people of both tribal and rural society of Assam from time immemorial. Therefore, the need of the hour is to harness this natural resource sustainably for the socio-economic development of the indigenous communities. Hence, a strategy for sustainable harvesting practice needs to be developed that would ensure preservation of the valuable medicinal plants in situ while addressing the needs of the rural communities. The present study is, thus, an attempt to highlight the common medicinal plants of forested region as used by the rural poor community for different kinds of treatment as the rural local healers usually practice for treatment of diseases in their locality.

  6. Stakeholder Perceptions of an Ecosystem Services Approach to Clearing Invasive Alien Plants on Private Land

    Directory of Open Access Journals (Sweden)

    Lauren S. Urgenson

    2013-03-01

    Full Text Available Incentivizing private landowners and other stakeholders is central to the effective conservation of ecosystem services in working landscapes. To better understand how to design effective incentives, the perceptions of landowners and other stakeholders are explored regarding a proposed approach to clearing invasive alien plants on private land in the Western Cape Province, South Africa. The public funded national program, Working for Water, conserves ecosystem services while employing and training people from marginalized sectors of society to clear these plants. Private landowner involvement is a key conservation challenge, because without adequate landowner involvement, invasive alien plants persist on the landscape and continuously reinvade cleared areas. We collected interview data from private landowners in three study sites, and web-survey data from conservation professionals and Working for Water managers, in order to compare stakeholder perceptions of (1 government and landowners' responsibilities for clearing invasive alien plants; (2 existing and proposed policy tools; and (3 the extent to which stakeholders consider the proposed financial incentive to be sufficient. There was significant consensus among stakeholders concerning their preference for shared landowner and government responsibility and for a policy mix that combines incentives with disincentives. Landowners from the three study sites differed in the level of responsibility they were willing to assume. Stakeholders also diverged in terms of their perceptions of the proposed financial incentives. Furthermore, the perspectives of landowners were strongly associated with ecological and social features of the landscapes in which they are located. Understanding stakeholders' points of view within their differing contexts is shown to be a valuable means of gaining insight into the opportunities and constraints that face ecosystem service conservation in working landscapes.

  7. A Multicriteria Approach for the Optimal Location of Gasoline Stations Being Transformed as Self-Service in Taiwan

    Directory of Open Access Journals (Sweden)

    Sheng-Pen Wang

    2016-01-01

    Full Text Available Location selection significantly influences business success. In particular, location selection for the fuel stations is characterized by constraints on investment in facilities and by criteria that involve a series of social utilities. Recently, a leading fuel company in Taiwan initiated transforming its traditional gas stations into self-service. However, it is difficult to select an existing station to be transformed as self-service because there are many conflicting goals in the problem of location selection. In this paper, we apply a multicriteria approach, integrating analytic hierarchy process (AHP and multichoice goal programming (MCGP, to obtain an appropriate gas station from many alternative locations that best suit the preferences of decision-makers in the case company. This study incorporates the weights obtained from AHP to set multiple aspirations in MCGP for ranking each candidate location. The results show that, under multiple quantitative and qualitative factors in the selection process, our proposed model is more scientific and efficient than unaided methods in finding a suitable location within a shorter evaluation time.

  8. Improved phytoaccumulation of cadmium by genetically modified tobacco plants (Nicotiana tabacum L.). Physiological and biochemical response of the transformants to cadmium toxicity

    Energy Technology Data Exchange (ETDEWEB)

    Gorinova, N. [AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164 Sofia (Bulgaria)]. E-mail: noraig60@yahoo.co.uk; Nedkovska, M. [AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164 Sofia (Bulgaria); Todorovska, E. [AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164 Sofia (Bulgaria); Simova-Stoilova, L. [Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113 Sofia (Bulgaria); Stoyanova, Z. [Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113 Sofia (Bulgaria); Georgieva, K. [Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113 Sofia (Bulgaria); Demirevska-Kepova, K. [Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113 Sofia (Bulgaria); Atanassov, A. [AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164 Sofia (Bulgaria); Herzig, R. [Phytotech-Foundation PT-F, Quartiergasse 12, CH 3013 Bern (Switzerland)

    2007-01-15

    The response of tobacco plants (Nicotiana tabacum L.)-non-transformed and transformed with a metallothionein gene MThis from Silene vulgaris L. - to increase cadmium supply in the nutrient solution was compared. The transgenic plants accumulated significantly more Cd both in the roots and the leaves. Visual toxicity symptoms and disturbance in water balance were correlated with Cd tissue content. Treatment with 300 {mu}M CdCl{sub 2} resulted in inhibition of photosynthesis and mobilization of the ascorbate-glutathione cycle. Treatment with 500 {mu}M CdCl{sub 2} led to irreversible damage of photosynthesis and oxidative stress. An appearance of a new peroxidase isoform and changes in the leaf polypeptide pattern were observed at the highest Cd concentration. The level of non-protein thiols gradually increased following the Cd treatment both in transgenic and non-transformed plants. - Genetic transformation of Nicotiana tabacum L. by metallothionein gene improved phytoaccumulation of cadmium.

  9. Conservation state of populations of rare plant species in highly transformed meadow steppes of Southern Opillya

    Directory of Open Access Journals (Sweden)

    I. I. Dmytrash-Vatseba

    2016-09-01

    Full Text Available Degradation of natural habitats causes rapid extinction of rare plant populations. The diversity of rare plant species in the meadow steppes of Southern Opillya (Western Ukraine depends strongly on patch area, pasture digression of vegetation and a variety of eco-coenotical conditions. The main threats for the rare components of the meadow steppe flora are reduction of habitat and overgrazing. Spatial connections between sites are unable to support a constant rare plant population. The analysis of the composition of rare plant meadow-steppe species indicated that habitats with similar rare species composition usually have similar parameters of area, stages of pasture digression and eco-coenotical conditions. Spatial connectivity of patches does not ensure species similarity of rare components of the flora. Rare plant species were grouped according to their preferences for habitat , area and condition. In small patches subject to any stage of pasture digression grow populations of Adonis vernalis L., Pulsatilla patens (L. Mill., P. grandis Wender., Stipa capillata L., S. рennata L., Chamaecytisus blockianus (Pawł. Klásková etc. On the contrary, populations of other species (Carlina onopordifolia Besser. ex Szafer., Kuecz. et Pawł., Adenophora liliifolia (L. Ledeb. ex A. DC., Crambe tataria Sebeók, Euphorbia volhynica Besser ex Racib., Stipa tirsa Stev. etc. prefer large habitats, not changed by pasture digression. Prevention of reduction of rare species diversity requires preservation (also extension of patch area and regulation of grazing intensity.

  10. Transformation of traditional knowledge of medicinal plants: the case of Tyroleans (Austria who migrated to Australia, Brazil and Peru

    Directory of Open Access Journals (Sweden)

    Pirker Heidemarie

    2012-11-01

    Full Text Available Abstract Background In ethnobotanical research, the investigation into traditional knowledge of medicinal plants in the context of migration has been of increasing interest in recent decades since it is influenced and changed by new environmental and social conditions. It most likely undergoes transformation processes to match the different living circumstances in the new location. This study compares the traditional knowledge of medicinal plants held by Tyroleans – and their descendants – who emigrated to Australia, Brazil and Peru at different time scales. The study’s findings allow a discussion of the complexities and dynamics that influence this knowledge within the context of long-distance migration. Methods Information was obtained from 65 informants by free-listing, semi-structured interviews and non-participatory observation in Tyrol (Austria and the migrants’ countries: Australia, Brazil and Peru. The collected data was analysed using different quantitative approaches, including statistical tests, and compared between the countries of investigation. Results All respondents in all four investigation areas claimed that they had knowledge and made use of medicinal plants to treat basic ailments in their day-to-day lives. Informants made 1,139 citations of medicinal plants in total in free lists, which correspond to 164 botanical taxa (genus or species level in Tyrol, 87 in Australia, 84 in Brazil and 134 in Peru. Of all the botanical taxa listed, only five (1.1% were listed in all four countries under investigation. Agreement among informants within free lists was highest in Tyrol (17%, followed by Peru (12.2%, Australia (11.9% and Brazil (11.2%. The proportion of agreement differs significantly between informants in Australia and Tyrol (p = 0.001, Brazil and Tyrol (p = 0.001 and Peru and Tyrol (p = 0.001 and is similar between informants in the migrant countries, as indicated by statistical tests. We recorded 1,286 use

  11. Services Innovation and the Transformation of Work: The Case of UK Telecommunications.

    Science.gov (United States)

    Miozzo, Marcela; Ramirez, Matias

    2003-01-01

    Customer service centers, call centers, engineering, and research and development divisions of a British telecommunications firm were examined to determine the effect of technological change on the development of competencies. Technology had different impacts on employment, career paths, skills, and competence development in different areas.…

  12. Transforming Monograph Collections with a Model of Collections as a Service

    Science.gov (United States)

    Way, Doug

    2017-01-01

    Financial pressures, changes in scholarly communications, the rise of online content, and the ability to easily share materials have provided libraries the opportunity to rethink their collections practices. This article provides an overview of these changes and outlines a framework for viewing collections as a service. It describes how libraries…

  13. Phase transformation and long-term service of high-temperature martensitic chromium steels

    Science.gov (United States)

    Kalashnikov, I. S.; Tarasenko, L.; Acselrad, O.; Pereira, L. C.; Shalkevich, A.; Soboleva, G.

    2001-02-01

    Martensitic high Cr (10 - 16%) steels alloyed with Ni (Co), Mo, W, V, and N are widely used in constructions subjected to cyclic loads at temperatures up to 600 degrees Celsius, in general after quenching from 1100 - 1150 degrees Celsius followed by tempering at 650 - 690 degrees Celsius. Due to long term service exposure at high temperatures, different microstructural changes take place, such as second-phases precipitation, formation of low-angle grain boundaries, as well as internal damage caused by cyclic loads and creep. Specific phase diagrams are presented that can be used to define time periods for reliable operation of parts with given composition, based on the time required for the appearance of second phase particles known to be detrimental to mechanical strength and performance. Restoring thermal treatments to be applied after long time exposure at service conditions, aiming at increasing service life, are also presented and discussed. The combined use of the diagrams and the restoring treatment ensures prediction of a reliable service-life period for components made of these steels.

  14. Corporate Learning in Times of Digital Transformation: A Conceptual Framework and Service Portfolio for the Learning Function in Banking Organisations

    Directory of Open Access Journals (Sweden)

    Daniela Schuchmann

    2015-03-01

    Full Text Available Digital transformation is one of the major challenges in all industries. It embraces the realignment of technology and new business models to more effectively engage digital customers at every touchpoint in the costumer experience lifecycle. Therefore, successful digital transformation begins with an understanding of digital consumer behavior, preferences and choices. It then leads to major consumer-centric changes within the organisation that address these needs. Such a consumer-centric and self-directed client structure has significant consequences for banks. Banks will need to continue existing services, while developing strategies to manage the shift in mix. For that reason, banking organisations are challenged to consider exploration new business fields and not only focus on exploitation anymore. Finally they need to ensure a successful balance between both. This means they have to establish a general development orientation and afford implementing innovations at the same time they focus on efficiency by utilizing and optimizing the existing. Pressure for doing this is definitely there and causes in current dynamic changes in the finance market, new competitors in the branch and little differentiation among offered banking services. Hence, they need to enhance their continuous learning ability, which is an essential precondition for coping with innovations. This paper follows the research question “how can the learning function foster the enhancement of the banking organisation’s learning and innovation ability in times of digital transformation?” This is closely linked to the kind of services a learning function needs to enhance or integrate for supporting and designing a learning organization. With the idea underlying enterprise 2.0, social media platforms offer valuable opportunities for doing this. From an educational management perspective, we focus on the design levels of individuals, teams, and organisation in banks. Derived

  15. Physical Metallurgy of Modern Creep-Resistant Steel for Steam Power Plants: Microstructure and Phase Transformations

    Directory of Open Access Journals (Sweden)

    V. C. Igwemezie

    2016-01-01

    Full Text Available The fact that the microstructure of steel depends on its composition and the heat treatment given to it has been heavily exploited in the design of steel for power plant applications. To obtain a steel that can function at the higher temperature where power plants operate without failure for extended life, heat treatment is needed to produce fine and highly stable dispersion of carbides, nitrides, and intermetallic compounds in the microstructure of the material. A significant contribution also comes from solid solution strengthening by substitutional solutes. We review here various types of phases, microstructures, functions, and interacting effects of the various alloying elements in the design of steel for modern power plant application.

  16. Role of plants in nitrogen and sulfur transformations in floating hydroponic root mats: A comparison of two helophytes.

    Science.gov (United States)

    Saad, Rania A B; Kuschk, Peter; Wiessner, Arndt; Kappelmeyer, Uwe; Müller, Jochen A; Köser, Heinz

    2016-10-01

    Knowledge about the roles helophytes play in constructed wetlands (CWs) is limited, especially regarding their provision of organic rhizodeposits. Here, transformations of inorganic nitrogen and sulfur were monitored in a CW variety, floating hydroponic root mat (FHRM), treating synthetic wastewater containing low concentration of organic carbon. Two helophytes, Phragmites australis and Juncus effusus, were compared in duplicates. Striking differences were found between the FHRM of the two helophytes. Whereas ammonium was removed in all FHRMs to below detection level, total nitrogen of 1.15 ± 0.4 g m(-2) d(-1) was removed completely only in P. australis systems. The mats with J. effusus displayed effective nitrification but incomplete denitrification as 77% of the removed ammonium-nitrogen accumulated as nitrate. Furthermore, the P. australis treatment units showed on average 3 times higher sulfate-S removal rates (1.1 ± 0.45 g m(-2) d(-1)) than the systems planted with J. effusus (0.37 ± 0.29 g m(-2) d(-1)). Since the influent organic carbon was below the stoichiometric requirement for the observed N and S transformation processes, helophytes' organic rhizodeposits apparently contributed to these transformations, while P. australis provided about 6 times higher bioavailable organic rhizodeposits than J. effusus. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. Using OAI-ORE to Transform Digital Repositories into Interoperable Storage and Services Applications

    Directory of Open Access Journals (Sweden)

    Steve Hitchcock

    2009-03-01

    Full Text Available In the digital age libraries are required to manage large numbers of diverse objects. One advantage of digital objects over fixed physical objects is the flexibility of 'binding' them into publications or other useful aggregated intellectual entities while retaining the ability to reuse them independently in other contexts. An emerging framework for managing flexible aggregations of digital objects is provided by the Open Archives Initiative (OAI with its work on Object Reuse and Exchange (ORE. This paper will show how OAI-ORE is being used to manage content in digital repositories, in particular institutional repositories, and has the potential ultimately to transform the conception of digital repositories.

  18. How tests of lubricating and transformer oils became part of power plant chemistry in Denmark

    Energy Technology Data Exchange (ETDEWEB)

    Moeller, H. [I/S Nordjyllandsvaerket, Vodskov (Denmark)

    1996-12-01

    Lubricating, hydraulic and transformer oils based on refined crude oil are used in nearly all power station components, such as gear, turbines, hydraulic stations, feed pumps and transformers. The function of these components totally depends on the condition of the oils and their properties. Seen from this point one may wonder why examination and evaluation of oils did not become part of the power station chemistry within the ELSAM utility area until during the middle of the eighties. We started to examine the properties of lubricating oils at the time when several steam turbines experienced serious problems with formation of deposits in their hydraulic control circuits. This work was intensified in connection with the significant number of CHPs and wind turbines erected within the Danish electricity sector during the past 10 years or so. The majority of the CHPs are natural gas fired turbines or motors, equipment which severely stresses the lubricating oil. In collaboration with KEMA, the Netherlands, we have carried through with a large examination of lubricating oils in gas turbines and we have found suitable oil types. The objectives of our work with lubricating and transformer oils have been to link together the laboratory measurements with operational experience. Only by doing this is it possible to utilize the laboratory measurements in a correct way. It must be remembered that the main part of all oil specifications concerns the properties of new oils. Only very little is published about the requirements concerning used oils. (EG)

  19. An improved plant regeneration and Agrobacterium - mediated transformation of red pepper (Capsicum annuum L.).

    Science.gov (United States)

    Kumar, R Vinoth; Sharma, V K; Chattopadhyay, B; Chakraborty, S

    2012-10-01

    Capsicum annuum (red pepper) is an important spice cum vegetable crop in tropical and subtropical countries. Here, we report an effective and reproducible auxin free regeneration method for six different red pepper cultivars (ACA-10, Kashi Anmol, LCA-235, PBC-535, Pusa Jwala and Supper) using hypocotyl explants and an efficient Agrobacterium-mediated transformation protocol. The explants (hypocotyls, cotyledonary leaves and leaf discs) collected from axenic seedlings of six red pepper cultivars were cultured on either hormone free MS medium or MS medium supplemented with BAP alone or in combination with IAA. Inclusion of IAA in the regeneration medium resulted in callus formation at the cut ends of explants, formation of rosette leaves and ill defined shoot buds. Regeneration of shoot buds could be achieved from hypocotyls grown in MS medium supplemented with different concentrations of BAP unlike other explants which failed to respond. Incorporation of GA3 in shoot elongation medium at 0.5 mg/l concentration enhanced the elongation in two cultivars, LCA-235 and Supper, while other cultivars showed no significant response. Chilli cultivar, Pusa Jwala was transformed with βC1 ORF of satellite DNA β molecule associated with Chilli leaf curl Joydebpur virus through Agrobacterium tumefaciens. Transgene integration in putative transformants was confirmed by PCR and Southern hybridization analysis.

  20. Enhancing Legume Ecosystem Services through an Understanding of Plant-Pollinator Interplay.

    Science.gov (United States)

    Suso, María J; Bebeli, Penelope J; Christmann, Stefanie; Mateus, Célia; Negri, Valeria; Pinheiro de Carvalho, Miguel A A; Torricelli, Renzo; Veloso, Maria M

    2016-01-01

    Legumes are bee-pollinated, but to a different extent. The importance of the plant-pollinator interplay (PPI), in flowering crops such as legumes lies in a combination of the importance of pollination for the production service and breeding strategies, plus the increasing urgency in mitigating the decline of pollinators through the development and implementation of conservation measures. To realize the full potential of the PPI, a multidisciplinary approach is required. This article assembles an international team of genebank managers, geneticists, plant breeders, experts on environmental governance and agro-ecology, and comprises several sections. The contributions in these sections outline both the state of the art of knowledge in the field and the novel aspects under development, and encompass a range of reviews, opinions and perspectives. The first three sections explore the role of PPI in legume breeding strategies. PPI based approaches to crop improvement can make it possible to adapt and re-design breeding strategies to meet both goals of: (1) optimal productivity, based on an efficient use of pollinators, and (2) biodiversity conservation. The next section deals with entomological aspects and focuses on the protection of the "pest control service" and pollinators in legume crops. The final section addresses general approaches to encourage the synergy between food production and pollination services at farmer field level. Two basic approaches are proposed: (a) Farming with Alternative Pollinators and (b) Crop Design System.

  1. Enhancing legume ecosystem services through an understanding of plant-pollinator interplay

    Directory of Open Access Journals (Sweden)

    Maria Jose eSuso

    2016-03-01

    Full Text Available Legumes are bee-pollinated, but to a different extent. The importance of the plant-pollinator interplay (PPI, in flowering crops such as legumes lies in a combination of the importance of pollination for the production service and breeding strategies, plus the increasing urgency in mitigating the decline of pollinators through the development and implementation of conservation measures. To realize the full potential of the PPI, a multidisciplinary approach is required. This article assembles an international team of genebank managers, geneticists, plant breeders, experts on environmental governance and agro-ecology, and comprises several sections. The contributions in these sections outline both the state of the art of knowledge in the field and the novel aspects under development, and encompass a range of reviews, opinions and perspectives. The first three sections explore the role of PPI in legume breeding strategies. PPI based approaches to crop improvement can make it possible to adapt and re-design breeding strategies to meet both goals of: 1 optimal productivity, based on an efficient use of pollinators, and 2 biodiversity conservation. The next section deals with entomological aspects and focuses on the protection of the pest control service and pollinators in legume crops. The final section addresses general approaches to encourage the synergy between food production and pollination services at farmer field level. Two basic approaches are proposed: a Farming with Alternative Pollinators (FAP and b Crop Design System (CDS.

  2. Assessing the fate and transformation of plant residues in the terrestrial environment using HR-MAS NMR spectroscopy

    Science.gov (United States)

    Kelleher, Brian P.; Simpson, Myrna J.; Simpson, Andre J.

    2006-08-01

    Plant litter decomposition plays a fundamental role in carbon and nitrogen cycles, provides key nutrients to the soil environment and represents a potentially large positive feedback to atmospheric CO 2. However, the full details of decomposition pathways and products are unknown. Here we present the first application of HR-MAS NMR spectroscopy on 13C and 15N labeled plant materials, and apply this approach in a preliminary study to monitor the environmental degradation of the pine and wheatgrass residues over time. In HR-MAS, is it possible to acquire very high resolution NMR data of plant biomass, and apply the vast array of multidimensional experiments available in conventional solution-state NMR. High levels of isotopic enrichment combined with HR-MAS significantly enhance the detection limits, and provide a wealth of information that is unattainable by any other method. Diffusion edited HR-MAS NMR data reveal the rapid loss of carbohydrate structures, while two-dimensional (2-D) HR-MAS NMR spectra demonstrate the relatively fast loss of both hydrolysable and condensed tannin structures from all plant tissues studied. Aromatic (partially lignin) and aliphatic components (waxes, cuticles) tend to persist, along with a small fraction of carbohydrate, and become highly functionalized over time. While one-dimensional (1-D) 13C HR-MAS NMR spectra of fresh plant tissue reflect compositional differences between pine and grass, these differences become negligible after decomposition suggesting that recalcitrant carbon may be similar despite the plant source. Two-dimensional 1H- 15N HR-MAS NMR analysis of the pine residue suggests that nitrogen from specific peptides is either selectively preserved or used for the synthesis of what appears to be novel structures. The amount of relevant data generated from plant components in situ using HR-MAS NMR is highly encouraging, and demonstrates that complete assignment will yield unprecedented structural knowledge of plant cell

  3. Gladiolus plants transformed with single-chain variable fragment antibodies to Cucumber mosaic virus

    Science.gov (United States)

    Transgenic plants of Gladiolus ‘Peter Pears’ or ‘Jenny Lee’ were developed that contain single-chain variable fragments (scFv) to Cucumber mosaic virus (CMV) subgroup I or II. The CMV subgroup I heavy and light chain scFv fragments were placed under control of either the duplicated CaMV 35S or suga...

  4. Mineralization and Transformation of Nitrogen Derived from Plant Materials in Soils over 10 Years

    Institute of Scientific and Technical Information of China (English)

    CHENGLI-LI; WENQI-XIAO

    1993-01-01

    Results of a 10-year decomposition experiment indicated that the annual mineralization rate of organic N in newly-formed humus varied with the type of original plant materials and the water regimes for decomposition,ranging from 0.028 to 0.074.The mineralization rate under waterlogged conditions was higher than that under upland conditions.The proportion of α-amino acid N in humus newly-formed under waterlogged conditions was slightly higher than that under upland conditions.It decreased gradually with time,while the proportion of nonhydrolyzable N showed no consistent trend,irrespective of the water regines for decomposition.The distribution of amino acids in humus newly-formed from different plant materials under various water regimes was quite similar with that in original plant materials,and only minor differences could be found among them.For example,in comparison to original plant materials,the newly-formed humus contained higher proportions of isoleucine,cysting,γ-amino-butyric acid and ornithine,and lower proportions of phenylalanine and proline.Moreover the proportion of phenylalanine was higher in the humus newly-fored under waterlogged conditions than that under upland conditions.

  5. 精益运营和业务转型%Exquisite Running and Services Transformation

    Institute of Scientific and Technical Information of China (English)

    赵平

    2005-01-01

    首先指出电信业界两个热点--精益运营(Lean Operation)和业务转型(Business Transformation),接着介绍了精益运营的含义及其内涵,并结合TMF eTOM初步提出如何量化精益运营指标的理念和思路.然后探讨了业务转型的思路和内容,以及业务转型和精益运营之间的关系,最后重点阐述了精益运营转型的思路之一--融合.

  6. Development of an efficient Agrobacterium-mediated transformation system and production of herbicide-resistant transgenic plants in garlic (Allium sativum L.).

    Science.gov (United States)

    Ahn, Yul-Kyun; Yoon, Moo-Kyoung; Jeon, Jong-Seong

    2013-08-01

    The genetic improvement of garlic plants (Allium sativum L.) with agronomical beneficial traits is rarely achieved due to the lack of an applicable transformation system. Here, we developed an efficient Agrobacterium-mediated transformation procedure with Danyang, an elite Korean garlic cultivar. Examination of sGFP (synthetic green fluorescence protein) expression revealed that treatment with 2-(N-morpholino) ethanesulfonic acid (MES), L-cysteine and/or dithiothreitol (DTT) gives the highest efficiency in transient gene transfer during Agrobacterium co-cultivation with calli derived from the roots of in vitro plantlets. To increase stable transformation efficiency, a two-step selection was employed on the basis of hygromycin resistance and sGFP expression. Of the hygromycin-resistant calli initially produced, only sGFP-expressing calli were subcultured for selection of transgenic calli. Transgenic plantlets produced from these calli were grown to maturity. The transformation efficiency increased up to 10.6% via our optimized procedure. DNA and RNA gel-blot analysis indicated that transgenic garlic plants stably integrated and expressed the phosphinothricin acetyltransferase (PAT) gene. A herbicide spraying assay demonstrated that transgenic plants of garlic conferred herbicide resistance, whilst nontransgenic plants and weeds died. These results indicate that our transformation system can be efficiently utilized to produce transgenic garlic plants with agronomic benefits.

  7. Second-Life Batteries on a Gas Turbine Power Plant to Provide Area Regulation Services

    Directory of Open Access Journals (Sweden)

    Lluc Canals Casals

    2017-03-01

    Full Text Available Batteries are used in the electricity grid to provide ancillary services. Area regulation seems to provide substantial revenues and profit, but Li-ion batteries are still too expensive to enter widely into this market. On the other hand, electric vehicle (EV batteries are considered inappropriate for traction purposes when they reach a state of health (SoH of 80%. The reuse of these batteries offers affordable batteries for second-life stationary applications. This study analyzes two possible scenarios where batteries may give power and energy support to a gas turbine cogeneration power plant, and how long these batteries may last under different loads.

  8. Coordinated system services from offshore wind power plants connected through HVDC networks

    DEFF Research Database (Denmark)

    Zeni, Lorenzo; Glasdam, Jakob; Hesselbæk, Bo;

    2014-01-01

    This paper presents an overview of power system services in networks involving multiple onshore power systems, a voltage sourced converter (VSC) based high voltage direct current (HVDC) offshore network and an offshore wind power plant (OWPP). A comprehensive list of services regarding onshore...... to their implementation. For example, new findings on onshore AC voltage control are reported, that help the characterisation of potential AC voltage control that a VSC-HVDC station may offer to an onshore AC grid. The HVDC system behind the VSC-HVDC station may connect, through other converters, to another AC power...... system, or an OWPP, or both. Moreover, the implementation of power oscillation damping (POD) and HVDC voltage control into an OWPP controller is proposed, discussing the main challenges related to their efficient design. Dynamic control challenges are assessed, in particular in relation to the inherent...

  9. Coordinated Control Scheme for Ancillary Services from Offshore Wind Power Plants to AC and DC Grids

    DEFF Research Database (Denmark)

    Sakamuri, Jayachandra N.; Altin, Müfit; Hansen, Anca Daniela;

    2016-01-01

    This paper proposes a new approach of providing ancillary services to AC and DC grids from offshore wind power plants (OWPPs), connected through multi-terminal HVDC network. A coordinated control scheme where OWPP’s AC grid frequency modulated according to DC grid voltage variations is used...... to detect and provide the ancillary service requirements of both AC and DC grids, is proposed in this paper. In particular, control strategies for onshore frequency control, fault ridethrough support in the onshore grid, and DC grid voltage control are considered. The proposed control scheme involves only...... local measurements and therefore avoids the need of communication infrastructure otherwise required for communication based control, and thus increases the reliability of the control system. The effectiveness of the proposed control scheme is demonstrated on a MTDC connected wind power system developed...

  10. A benchmark simulation model to describe plant-wide phosphorus transformations in WWTPs

    DEFF Research Database (Denmark)

    Flores-Alsina, Xavier; Ikumi, D.; Kazadi-Mbamba, C.

    It is more than 10 years since the publication of the BSM1 technical report (Copp, 2002). The main objective of BSM1 was to create a platform for benchmarking C and N removal strategies in activated sludge systems. The initial platform evolved into BSM1_LT and BSM2, which allowed for the evaluati...... to be addressed and presents the simulation results of the first software prototype....... of monitoring and plant-wide control strategies, respectively. In addition, researchers working within the IWA Task Group on Benchmarking of Control Strategies for Wastewater Treatment Plants developed other BSM related spin-off products, such as the dynamic influent generator, sensor/actuators/fault models......) pursue biological/chemical phosphorus removal. However, realistic descriptions of combined C, N and P removal, adds a major, but unavoidable degree of complexity in wastewater treatment process models. This paper identifies and discusses important issues that need to be addressed to upgrade the BSM2...

  11. Development of a Pollination Service Measurement (PSM) method using potted plant phytometry.

    Science.gov (United States)

    Woodcock, Thomas S; Pekkola, Laura J; Dawson, Cara; Gadallah, Fawziah L; Kevan, Peter G

    2014-08-01

    The value of pollination to human society is not limited to agricultural production, but also in the sustainability of ecosystems and the services that they provide. Seed set can be used as a comparative measure of pollination effectiveness, with minimum variability expected when other resources are not limiting. Six species of self-incompatible fall asters (Symphyotrichum) were used to evaluate pollination service at 12 sites across a spectrum of expected levels of pollination. Seed set per inflorescence was generally lower at sites with lower pollinator numbers and diversity, although as expected pollinator assemblage characteristics were highly variable within and between sites. However, rankings of sites showed consistency of response across phytometer species and between years; the summed ranks across multiple species appears to have as the greatest value in Pollination Service Measurement (PSM). Abundance, richness, and Shannon diversity of pollinator assemblages were highly autocorrelated and showed variable relationships with seed set depending on plant species and temporal scale of pollinator assemblage assessment. Use of seed set to directly measure pollination service at a site was consistent and cost effective when compared to less certain and more labour-intensive methods of pollinator collection and identification, and shows promise for implementation in pollination monitoring and bioassessment practices.

  12. Engaging, critical, entertaining: Transforming public service television for children in Denmark

    DEFF Research Database (Denmark)

    Christensen, Christa Lykke

    2013-01-01

    of children’s television can be identified, leading up to today’s competitive period. The article shows how Danish public service children’s TV has played an important role in the modernization of the view of children, serving as an experimental platform for putting the perspective of active and independent...... children on the agenda. The argument is that this agenda has been contested by the increased demands for entertaining content due to increased competition among TV channels and due to obeying demands for ever more content to complete programming schedules....

  13. [Capital accumulation patterns: capital and services in the current transformations of the health system].

    Science.gov (United States)

    de Andreazzi, Maria de Fátima Siliansky; Kornis, George Edward Machado

    2008-01-01

    The purpose of this article is to introduce elements of the capitalist society economic reproduction to the discussion around the current dynamics of health accumulation. It identifies the direction and significance of capital accumulation in the health area as well as the characteristics of the economic environment where the competition currently takes place. The hypothetic hypertrophy of the financial sphere is seen as a means for structuring the capitalist economy since the late twentieth century. The former delimitations between industrial production and service delivery are blurred and weakened; the competition process shows new features and the contradictions - internally between the different elements of the health industry and externally with other sectors - are changing. This article aggregates elements for a contemporary analysis of the "medical-industrial complex" on the basis of a theoretical-historical-conceptual approach. We identify changes in the capital dynamics of this complex at international and national level and stress the increasing role of the health services as a forefront of capital accumulation. The new approach is based on the economic thinking of Marx in addition to the current discussions about the theory of financial capital accumulation and the new productive configurations of the large corporations.

  14. Multiple abiotic stress tolerance of the transformants yeast cells and the transgenic Arabidopsis plants expressing a novel durum wheat catalase.

    Science.gov (United States)

    Feki, Kaouthar; Kamoun, Yosra; Ben Mahmoud, Rihem; Farhat-Khemakhem, Ameny; Gargouri, Ali; Brini, Faiçal

    2015-12-01

    Catalases are reactive oxygen species scavenging enzymes involved in response to abiotic and biotic stresses. In this study, we described the isolation and functional characterization of a novel catalase from durum wheat, designed TdCAT1. Molecular Phylogeny analyses showed that wheat TdCAT1 exhibited high amino acids sequence identity to other plant catalases. Sequence homology analysis showed that TdCAT1 protein contained the putative calmodulin binding domain and a putative conserved internal peroxisomal targeting signal PTS1 motif around its C-terminus. Predicted three-dimensional structural model revealed the presence of four putative distinct structural regions which are the N-terminal arm, the β-barrel, the wrapping and the α-helical domains. TdCAT1 protein had the heme pocket that was composed by five essential residues. TdCAT1 gene expression analysis showed that this gene was induced by various abiotic stresses in durum wheat. The expression of TdCAT1 in yeast cells and Arabidopsis plants conferred tolerance to several abiotic stresses. Compared with the non-transformed plants, the transgenic lines maintained their growth and accumulated more proline under stress treatments. Furthermore, the amount of H2O2 was lower in transgenic lines, which was due to the high CAT and POD activities. Taken together, these data provide the evidence for the involvement of durum wheat catalase TdCAT1 in tolerance to multiple abiotic stresses in crop plants. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  15. Analysis of base content in in-service oils by fourier transform infrared spectroscopy.

    Science.gov (United States)

    Ehsan, Sadia; Sedman, Jacqueline; van de Voort, Frederick R; Akochi-Koblé, Emmanuel; Yuan, Tao; Takouk, Djaouida

    2012-06-01

    An automated FTIR method for the determination of the base content (BC(pKa)) of oils at rates of > 120 samples/h has been developed. The method uses a 5% solution of trifluoroacetic acid in 1-propanol (TFA/P) added to heptane-diluted oil to react with the base present and measures the ν(COO(-)) absorption of the TFA anion produced, with calibrations devised by gravimetrically adding 1-methylimidazole to a heptane-TFA/P mixture. To minimize spectral interferences, all spectra are transformed to 2(nd) derivative spectra using a gap-segment algorithm. Any solvent displacement effects resulting from sample miscibility are spectrally accounted for by measurement of the changes in the 1-propanol overtone band at 1936 cm(-1). A variety of oils were analyzed for BC(0.5), expressed as mEq base/g oil as well as converted to base number (BN) units (mg KOH/g oil) to facilitate direct comparison with ASTM D2896 and ASTM D974 results for the same samples. Linear relationships were obtained between FTIR and D2896 and D974, with the ASTM methods producing higher BN values by factors of ~1.5 and ~1.3, respectively. Thus, the FTIR BC method correlates well with ASTM potentiometric procedures and, with its much higher throughput, promises to be a useful alternative means of rapidly determining reserve alkalinity in commercial oil condition monitoring laboratories.

  16. Fourier transform mid infrared spectroscopy applications for monitoring the structural plasticity of plant cell walls

    Science.gov (United States)

    Largo-Gosens, Asier; Hernández-Altamirano, Mabel; García-Calvo, Laura; Alonso-Simón, Ana; Álvarez, Jesús; Acebes, José L.

    2014-01-01

    Fourier transform mid-infrared (FT-MIR) spectroscopy has been extensively used as a potent, fast and non-destructive procedure for analyzing cell wall architectures, with the capacity to provide abundant information about their polymers, functional groups, and in muro entanglement. In conjunction with multivariate analyses, this method has proved to be a valuable tool for tracking alterations in cell walls. The present review examines recent progress in the use of FT-MIR spectroscopy to monitor cell wall changes occurring in muro as a result of various factors, such as growth and development processes, genetic modifications, exposition or habituation to cellulose biosynthesis inhibitors and responses to other abiotic or biotic stresses, as well as its biotechnological applications. PMID:25071791

  17. Fourier transform Raman spectroscopic characterisation of cells of the plant-associated soil bacterium Azospirillum brasilense Sp7

    Science.gov (United States)

    Kamnev, A. A.; Tarantilis, P. A.; Antonyuk, L. P.; Bespalova, L. A.; Polissiou, M. G.; Colina, M.; Gardiner, P. H. E.; Ignatov, V. V.

    2001-05-01

    Structural and compositional features of bacterial cell samples and of lipopolysaccharide-protein complex isolated from the cell surface of the plant-growth-promoting rhizobacterium Azospirillum brasilense (wild-type strain Sp7) were characterised using Fourier transform (FT) Raman spectroscopy. The structural spectroscopic information obtained is analysed and considered together with analytical data on the content of metal cations (Co 2+, Cu 2+ and Zn 2+) in the bacterial cells grown in a standard medium as well as in the presence of each of the cations (0.2 mM). The latter, being taken up by bacterial cells from the culture medium in significant amounts, were shown to induce certain metabolic changes in the bacterium revealed in FT-Raman spectra, which is discussed from the viewpoint of bacterial response to environmental stresses.

  18. Soil ni.trogen transformations varied with plant community under Nanchang urban forests in mid-subtropical zone of China

    Institute of Scientific and Technical Information of China (English)

    REN Wen; CHEN Fu-sheng; HU Xiao-fei; YU Ming-quan; FENG Xue

    2011-01-01

    Soil N transformations using the polyvinyl chloride (PVC)closed-top tube in situ incubation method were studied in Nanchang urban forests of the mid-subtropical region of China m different monthsof 2007.Four plots of 20 m × 20 m were established in four different plant communities that represented typical successional stages of forest development including shrubs,coniferous forest,mixed forest and broadleaved forest.Average concentrations of soil NH4+-N fiom January to December were not different among the four plant communities.The concentrations of soil NO3-N and mineral N,and the annual rates of ammonification,nitrification and net N-mineralization under the early successional shrub community and coniferous forest were generally lower than that of the late successional mixed and broad-leaved forests (p<0.05).Similar differences among the plant communities were also shown in the relative nitrification index (NH4+-N/NO3-N) and relative nitrification intensity (nitrification rate/net N-mineralization rate).The annual net N-mineralization rate was increased from younger to older plant communities,from 15.1 and 41.4 kg·ha-1·a-1 under the shrubs and coniferous forest communities to 98.0 and 112.9 kg·ha-1·a-1 under the mixed and broad-leaved forests,respectively.Moreover,the high annual nitrification rates (50-70 kg·ha-1·a-1) and its end product,NO3-N (2.4-3.8mg·kg-1),under older plant communities could increase the potential risk of N loss.Additionally,the temporal patterns of the different soil N variables mentioned above varied with different plant community due to the combined affects of natural biological processes associated with forest maturation and urbanization.Our results indicated that urban forests arc moving towards a state of"N saturation" (extremely nitrification rate and NO3-N content) as they mature.

  19. Expression of anti-tumor necrosis factor alpha (TNFα) single-chain variable fragment (scFv) in Spirodela punctata plants transformed with Agrobacterium tumefaciens.

    Science.gov (United States)

    Balaji, Parthasarathy; Satheeshkumar, P K; Venkataraman, Krishnan; Vijayalakshmi, M A

    2016-05-01

    Therapeutic antibodies against tumor necrosis factor alpha (TNFα) have been considered effective for some of the autoimmune diseases such as rheumatoid arthritis, Crohn's diseases, and so on. But associated limitations of the current therapeutics in terms of cost, availability, and immunogenicity have necessitated the need for alternative candidates. Single-chain variable fragment (scFv) can negate the limitations tagged with the anti-TNFα therapeutics to a greater extent. In the present study, Spirodela punctata plants were transformed with anti-TNFα through in planta transformation using Agrobacterium tumefaciens strain, EHA105. Instead of cefotaxime, garlic extract (1 mg/mL) was used to remove the agrobacterial cells after cocultivation. To the best of our knowledge, this report shows for the first time the application of plant extracts in transgenic plant development. 95% of the plants survived screening under hygromycin. ScFv cDNA integration in the plant genomic DNA was confirmed at the molecular level by PCR. The transgenic protein expression was followed up to 10 months. Expression of scFv was confirmed by immunodot blot. Protein expression levels of up to 6.3% of total soluble protein were observed. β-Glucuronidase and green fluorescent protein expressions were also detected in the antibiotic resistant plants. The paper shows the generation of transgenic Spirodela punctuata plants through in planta transformation.

  20. Effect of plant age, larval age, and fertilizer treatment on resistance of a cry1Ab-transformed aromatic rice to lepidopterous stem borers and foliage feeders.

    Science.gov (United States)

    Alinia, F; Ghareyazie, B; Rubia, L; Bennett, J; Cohen, M B

    2000-04-01

    The resistance of vegetative, booting, and flowering stage plants of a variety of an aromatic rice, Oryza sativa L., transformed with a Bacillus thuringiensis Berliner cry1Ab gene under control of the maize phosphoenolpyruvate carboxylase (PEPC) promoter was evaluated against four lepidopterous rice pests--the stem borers Chilo suppressalis (Walker) (Lepidoptera: Crambidae) and Scirpophaga incertulas (Walker) (Lepidoptera: Pyralidae), and the foliage feeders Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae) and Naranga aenescens Moore (Lepidoptera: Noctuidae). Plants of the cry1Ab-transformed line (no. 827) were more resistant to young larvae of S. incertulas, C. suppressalis, and C. medinalis than control plants at the vegetative stage but not at the flowering stage. Survival of 10-d-old stem borer larvae did not differ on cry1Ab plants and control plants at either the vegetative or flowering stage, but the development of 10-d-old C. suppressalis larvae was retarded on the vegetative stage cry1Ab plants. Immunological analysis also showed an apparent decline in Cry1Ab titer in leaf blades and leaf sheaths at the reproductive stage. In experiments comparing three fertilizer treatments (NPK, PK, and none), there was a significant interaction between fertilizer treatment and variety on larval survival only in whole-plant assays at booting stage with C. suppressalis. On cry1Ab plants, larval survival did not differ significantly among the three fertilizer levels, whereas on control plants survival was highest with the NPK treatment. cry1Ab plants tested at the sixth and seventh generations after transformation were more resistant than control plants to N. aenescens and C. suppressalis, respectively, suggesting that gene silencing will not occur in line 827. The results of the experiments are discussed in terms of resistance management for B. thuringiensis toxins in rice.

  1. Agrobacterium-mediated transformation of apple (Malus x domestica Borkh.): an assessment of factors affecting regeneration of transgenic plants.

    Science.gov (United States)

    De Bondt, A; Eggermont, K; Penninckx, I; Goderis, I; Broekaert, W F

    1996-03-01

    We have previously developed a protocol for efficient gene transfer and regeneration of transgenic calli following cocultivation of apple (cv. Jonagold) explants with Agrobacterium tumefaciens (De Bondt et al. 1994, Plant Cell Reports 13: 587-593). Now we report on the optimization of postcultivation conditions for efficient and reproducible regeneration of transgenic shoots from the apple cultivar Jonagold. Factors which were found to be essential for efficient shoot regeneration were the use of gelrite as a gelling agent and the use of the cytokinin-mimicing thidiazuron in the selective postcultivation medium. Improved transformation efficiencies were obtained by combining the hormones thidiazuron and zeatin and by using leaf explants from in vitro grown shoots not older than 4 weeks after multiplication. Attempts to use phosphinothricin acetyl transferase as a selectable marker were not successful. Using selection on kanamycin under optimal postcultivation conditions, about 2% of the leaf explants developed transgenic shoots or shoot clusters. The presence and expression of the transferred genes was verified by β-glucuronidase assays and Southern analysis. The transformation procedure has also been succesfully applied to several other apple cultivars.

  2. Attenuation of pharmaceuticals and their transformation products in a wastewater treatment plant and its receiving river ecosystem.

    Science.gov (United States)

    Aymerich, I; Acuña, V; Barceló, D; García, M J; Petrovic, M; Poch, M; Rodriguez-Mozaz, S; Rodríguez-Roda, I; Sabater, S; von Schiller, D; Corominas, Ll

    2016-09-01

    Pharmaceuticals are designed to improve human and animal health, but may also be a threat to freshwater ecosystems, particularly after receiving urban or wastewater treatment plant (WWTP) effluents. Knowledge on the fate and attenuation of pharmaceuticals in engineered and natural ecosystems is rather fragmented, and comparable methods are needed to facilitate the comprehension of those processes amongst systems. In this study the dynamics of 8 pharmaceuticals (acetaminophen, sulfapyridine, sulfamethoxazole, carbamazepine, venlafaxine, ibuprofen, diclofenac, diazepam) and 11 of their transformation products were investigated in a WWTP and the associated receiving river ecosystem. During 3 days, concentrations of these compounds were quantified at the influents, effluents, and wastage of the WWTP, and at different distances downstream the effluent at the river. Attenuation (net balance between removal and release from and to the water column) was estimated in both engineered and natural systems using a comparable model-based approach by considering different uncertainty sources (e.g. chemical analysis, sampling, and flow measurements). Results showed that pharmaceuticals load reduction was higher in the WWTP, but attenuation efficiencies (as half-life times) were higher in the river. In particular, the load of only 5 out of the 19 pharmaceuticals was reduced by more than 90% at the WWTP, while the rest were only partially or non-attenuated (or released) and discharged into the receiving river. At the river, only the load of ibuprofen was reduced by more than 50% (out of the 6 parent compounds present in the river), while partial and non-attenuation (or release) was observed for some of their transformation products. Linkages in the routing of some pharmaceuticals (venlafaxine, carbamazepine, ibuprofen and diclofenac) and their corresponding transformation products were also identified at both WWTP and river. Finally, the followed procedure showed that dynamic

  3. The taxonomic name resolution service: an online tool for automated standardization of plant names

    Directory of Open Access Journals (Sweden)

    Boyle Brad

    2013-01-01

    Full Text Available Abstract Background The digitization of biodiversity data is leading to the widespread application of taxon names that are superfluous, ambiguous or incorrect, resulting in mismatched records and inflated species numbers. The ultimate consequences of misspelled names and bad taxonomy are erroneous scientific conclusions and faulty policy decisions. The lack of tools for correcting this ‘names problem’ has become a fundamental obstacle to integrating disparate data sources and advancing the progress of biodiversity science. Results The TNRS, or Taxonomic Name Resolution Service, is an online application for automated and user-supervised standardization of plant scientific names. The TNRS builds upon and extends existing open-source applications for name parsing and fuzzy matching. Names are standardized against multiple reference taxonomies, including the Missouri Botanical Garden's Tropicos database. Capable of processing thousands of names in a single operation, the TNRS parses and corrects misspelled names and authorities, standardizes variant spellings, and converts nomenclatural synonyms to accepted names. Family names can be included to increase match accuracy and resolve many types of homonyms. Partial matching of higher taxa combined with extraction of annotations, accession numbers and morphospecies allows the TNRS to standardize taxonomy across a broad range of active and legacy datasets. Conclusions We show how the TNRS can resolve many forms of taxonomic semantic heterogeneity, correct spelling errors and eliminate spurious names. As a result, the TNRS can aid the integration of disparate biological datasets. Although the TNRS was developed to aid in standardizing plant names, its underlying algorithms and design can be extended to all organisms and nomenclatural codes. The TNRS is accessible via a web interface at http://tnrs.iplantcollaborative.org/ and as a RESTful web service and application programming interface. Source code

  4. The taxonomic name resolution service: an online tool for automated standardization of plant names.

    Science.gov (United States)

    Boyle, Brad; Hopkins, Nicole; Lu, Zhenyuan; Raygoza Garay, Juan Antonio; Mozzherin, Dmitry; Rees, Tony; Matasci, Naim; Narro, Martha L; Piel, William H; McKay, Sheldon J; Lowry, Sonya; Freeland, Chris; Peet, Robert K; Enquist, Brian J

    2013-01-16

    The digitization of biodiversity data is leading to the widespread application of taxon names that are superfluous, ambiguous or incorrect, resulting in mismatched records and inflated species numbers. The ultimate consequences of misspelled names and bad taxonomy are erroneous scientific conclusions and faulty policy decisions. The lack of tools for correcting this 'names problem' has become a fundamental obstacle to integrating disparate data sources and advancing the progress of biodiversity science. The TNRS, or Taxonomic Name Resolution Service, is an online application for automated and user-supervised standardization of plant scientific names. The TNRS builds upon and extends existing open-source applications for name parsing and fuzzy matching. Names are standardized against multiple reference taxonomies, including the Missouri Botanical Garden's Tropicos database. Capable of processing thousands of names in a single operation, the TNRS parses and corrects misspelled names and authorities, standardizes variant spellings, and converts nomenclatural synonyms to accepted names. Family names can be included to increase match accuracy and resolve many types of homonyms. Partial matching of higher taxa combined with extraction of annotations, accession numbers and morphospecies allows the TNRS to standardize taxonomy across a broad range of active and legacy datasets. We show how the TNRS can resolve many forms of taxonomic semantic heterogeneity, correct spelling errors and eliminate spurious names. As a result, the TNRS can aid the integration of disparate biological datasets. Although the TNRS was developed to aid in standardizing plant names, its underlying algorithms and design can be extended to all organisms and nomenclatural codes. The TNRS is accessible via a web interface at http://tnrs.iplantcollaborative.org/ and as a RESTful web service and application programming interface. Source code is available at https://github.com/iPlantCollaborativeOpenSource/TNRS/.

  5. Impact of Production from Photovoltaic Power Plants on Increase of Ancillary Services in the Czech Republic

    Directory of Open Access Journals (Sweden)

    Martin Smocek

    2016-01-01

    Full Text Available Renewable energy resources represent a noticeable part of the overall energetic concept development. New integration of renewable energy resources into power grids has a significant impact on the reliability and quality of power supply. The major problem of the photovoltaic and wind power plants is their dependency on weather conditions, since it has a direct effect on their immediate output produced that shows stochastic behaviour. These stochastic outputs result in very adverse impacts on the power grid. Further development of these resources could lead to exceeding of the control and absorption abilities of the power grid. The power grid must be set in balance with respect to the production and consumption of electric power at any time. The operation of photovoltaic power plants impair keeping this balance. That has an adverse impact on the very operation and maintenance of network parameters within the extent required. This survey deals with analysis focused on operation of the photovoltaic power plants with respect to the increase of reserve power in ancillary services in the Czech Republic.

  6. Summary of the research and development effort on steam plants for electric-utility service

    Energy Technology Data Exchange (ETDEWEB)

    Fraas, A.P.

    1981-06-01

    The development of steam power plants for electric utility service over the past century is reviewed with particular emphasis on the prime problems and their solution. Increases in steam pressure and temperature made possible by developments in metallurgy led to an increase in thermal efficiency by a factor of 8 between 1880 and 1955. Further improvements have not been made because the use of still more expensive alloys is not economically justified, even with the much higher fuel prices of the latter 1970's. In fact, EPA regulations on waste heat and sulfur emissions have led to the use of cooling towers and wet limestone stack gas scrubbers that cause a degradation in plant thermal efficiency. The various possibilities for further improvements in efficiency and their problems are examined. The development of steam power plants in the past has been carried out in sufficiently small steps that the utilities and the equipment manufacturers have been able to assume the financial risk involved; but the fluidized-bed combustion system, which appears to be the most promising area, presents such a large step with major uncertainties that U.S. government financial support of the research and development effort appears to be required. The potential benefits appear to justify the research and development cost many times over.

  7. Ecosystem Services and Abrupt Transformations in a Coastal Wetland Social-Ecological System: Tubul-Raqui after the 2010 Earthquake in Chile

    Directory of Open Access Journals (Sweden)

    Andrés Marín

    2014-03-01

    Full Text Available Natural disasters can trigger sudden transformations and move ecosystems to different states where the provision of ecosystem services is altered. These changes in ecosystem services affect local communities' well-being and challenge users' adaptation capacities. We used the ecosystem services framework to understand the impacts of abrupt transformations, in a coastal wetland, associated to a ~ 1.6 meter coseismic uplift after an 8.8 magnitude earthquake in Chile. Using mixed methods we (1 identified and prioritized ecosystem services from Tubul-Raqui wetland; (2 assessed conditions of services and human well-being before and after the earthquake; (3 investigated postcatastrophe human adaptations and responses; and (4 explored users' interests and visions about possible future social-ecological pathways. Results show spatially diversified effects of the uplift on ecosystem services, both negative and positive, representing threats and opportunities for different user groups around the wetland. The total loss of the cultivated seaweed "pelillo" is associated with the most manifest reduction in perceptions of well-being among coastal users. Adaptive capacities triggered by pre-existing livelihood portfolios generated intensification in the exploitation of less impacted or enhanced ecosystem services which could be reducing resilience. Results show that two years after the transformation there is little attempt to create untried, new beginnings in the Tubul-Raqui wetland from which user groups could evolve to a more innovative livelihood and resource management system after the shift. Although visions about the future are not homogeneous among users, common interests regarding the conservation of key services are shared. The analysis of abrupt transformations through an ecosystem services approach provides a powerful framework for the study of environmental change and associated impacts on local communities.

  8. Enhanced transformation of TNT by Arabidopsis plants expressing an old yellow enzyme.

    Science.gov (United States)

    Zhu, Bo; Peng, Ri-He; Fu, Xiao-Yan; Jin, Xiao-Fen; Zhao, Wei; Xu, Jing; Han, Hong-Juan; Gao, Jian-Jie; Xu, Zhi-Sheng; Bian, Lin; Yao, Quan-Hong

    2012-01-01

    2,4,6-Trinitrotoluene (TNT) is released in nature from manufacturing or demilitarization facilities, as well as after the firing or detonation of munitions or leakage from explosive remnants of war. Environmental contamination by TNT is associated with human health risks, necessitating the development of cost-effective remediation techniques. The lack of affordable and effective cleanup technologies for explosives contamination requires the development of better processes. In this study, we present a system for TNT phytoremediation by overexpressing the old yellow enzyme (OYE3) gene from Saccharomyces cerevisiae. The resulting transgenic Arabidopsis plants demonstrated significantly enhanced TNT tolerances and a strikingly higher capacity to remove TNT from their media. The current work indicates that S. cerevisiae OYE3 overexpression in Arabidopsis is an efficient method for the phytoremoval and degradation of TNT. Our findings have the potential to provide a suitable remediation strategy for sites contaminated by TNT.

  9. Enhanced transformation of TNT by Arabidopsis plants expressing an old yellow enzyme.

    Directory of Open Access Journals (Sweden)

    Bo Zhu

    Full Text Available 2,4,6-Trinitrotoluene (TNT is released in nature from manufacturing or demilitarization facilities, as well as after the firing or detonation of munitions or leakage from explosive remnants of war. Environmental contamination by TNT is associated with human health risks, necessitating the development of cost-effective remediation techniques. The lack of affordable and effective cleanup technologies for explosives contamination requires the development of better processes. In this study, we present a system for TNT phytoremediation by overexpressing the old yellow enzyme (OYE3 gene from Saccharomyces cerevisiae. The resulting transgenic Arabidopsis plants demonstrated significantly enhanced TNT tolerances and a strikingly higher capacity to remove TNT from their media. The current work indicates that S. cerevisiae OYE3 overexpression in Arabidopsis is an efficient method for the phytoremoval and degradation of TNT. Our findings have the potential to provide a suitable remediation strategy for sites contaminated by TNT.

  10. Reacting flow simulations of supercritical water oxidation of PCB-contaminated transformer oil in a pilot plant reactor

    Directory of Open Access Journals (Sweden)

    V. Marulanda

    2011-06-01

    Full Text Available The scale-up of a supercritical water oxidation process, based on recent advancements in kinetic aspects, reactor configuration and optimal operational conditions, depends on the research and development of simulation tools, which allow the designer not only to understand the complex multiphysics phenomena that describe the system, but also to optimize the operational parameters to attain the best profit for the process and guarantee its safe operation. Accordingly, this paper reports a multiphysics simulation with the CFD software Comsol Multiphysics 3.3 of a pilot plant reactor for the supercritical water oxidation of a heavily PCB-contaminated mineral transformer oil. The proposed model was based on available information for the kinetic aspects of the complex mixture and the optimal operational conditions obtained in a lab-scale continuous supercritical water oxidation unit. The pilot plant simulation results indicate that it is not feasible to scale-up directly the optimal operational conditions obtained in the isothermal lab-scale experiments, due to the excess heat released by the exothermic oxidation reactions that result in outlet temperatures higher than 600°C, even at reactor inlet temperatures as low as 400°C. Consequently, different alternatives such as decreasing organic flowrates or a new reactor set-up with multiple oxidant injections should be considered to guarantee a safe operation.

  11. Authentic Research Experiences for Pre- and In-service Teachers: UNH’s Transforming Earth System Science Education (TESSE) Program

    Science.gov (United States)

    Varner, R. K.; Graham, K.; Bryce, J. G.; Finkel, L.; Froburg, E.; Hale, S.; Johnson, J. E.; von Damm, K.

    2009-12-01

    The University of New Hampshire’s Transforming Earth System Science Education (TESSE) project is designed to enrich the education and professional development of in-service and pre-service teachers who currently teach or plan to teach Earth science curricula. A key TESSE program goal is to foster the development of middle and high school students’ Earth System Science (ESS) literacy by engaging teachers in authentic research experiences. As part of TESSE, pre-service teachers are engaged in a research immersion experience (RIE), during which they work alongside a faculty research mentor for an intensive eight week period. The chief goal of this component of the program is to provide these pre-service teachers with authentic research experiences that will sharpen their research skills by providing guidance in designing projects and in gathering and interpreting data. The RIE also includes weekly discussions on pedagogical approaches to research with students, providing a catalyst for taking these research experiences into the classroom. For in-service teachers we have developed a course: “Research Techniques in the Earth System Sciences for Teachers.” Teachers enroll in an intensive ten-day research experience during which they participate in a research cruise on the Great Bay Estuary and collect gas and water samples, measure parameters in the water column, and collect sediment cores in order to answer questions they have posed about the estuary environment. Onshore, teacher teams analyze the water and core samples for gas and trace metal content, grain size, and other sedimentological characteristics. The research experience culminates with a public poster presentation of each team's results. Throughout the experience, teachers are engaged in a variety of discussions about transferring what they have learned about doing research to their classrooms. The integration of authentic research is a key mechanism that the TESSE program uses to help teachers

  12. Transformation of the multidisciplinary diabetic foot clinic into a multidisciplinary diabetic foot day unit: results from a service evaluation.

    Science.gov (United States)

    Manu, Chris A; Mustafa, Omar G; Bates, Maureen; Vivian, Gill; Mulholland, Nicola; Elias, David; Huang, Dean Y; Deane, Colin; Cavale, Naveen; Kavarthapu, Venu; Rashid, Hisham; Edmonds, Michael

    2014-09-01

    The natural history of the diabetic foot is aggressive and complex. To counteract this, we describe the transformation of a Multidisciplinary Diabetic Foot Clinic into a Multidisciplinary Diabetic Foot Day Unit, which delivers an emergency open access system for patients, with a "one-stop," same day service in which investigations are performed, results reviewed and treatment implemented. It also provides joint clinics with vascular, orthopaedic, and plastic surgeons and specialized clinics for casting of complex neuropathic feet and for the administration of intravenous or intramuscular antibiotics on the same day. The aim was to document these increasingly wide-ranging facilities by undertaking a retrospective evaluation over a 6-week period, with analysis of notes, investigations, and an anonymous patient satisfaction survey. The clinic was visited by 597 patients who attended in 1076 appointments, of which 112 (10.4%) were emergency visits; these patients attended the clinic without a booked appointment but via an open access policy, 93 of whom were known to the clinic, but 19 were new self-referred patients to the service. Furthermore, 197 (18%) were seen in a Joint Vascular Diabetic Foot Clinic and 98 (9%) were seen in a Joint Orthopaedic Plastic Diabetic Foot Clinic, 570 (53%) were seen in an Active Ulcer Clinic and 97 (9%) in a Total Contact Casting Clinic. Forty-five percent of patients were prescribed antibiotics, including 188 (76%) as oral and 45(18%) as intravenous antibiotics and 15(6%) as intramuscular injections. Of the 1076 appointments, 150 (14%) patients were in the foot clinic for more than 4 hours. Sixty (10%) patients were reviewed 4 or more times over the 6-week period. Only 22 (2%) were admitted to hospital. Of the 125 survey responders, 98% were satisfied with this service, which has evolved from a Diabetic Foot Clinic into a Multidisciplinary Diabetic Foot Day Unit.

  13. Transformation of traditional knowledge of medicinal plants: the case of Tyroleans (Austria) who migrated to Australia, Brazil and Peru.

    Science.gov (United States)

    Pirker, Heidemarie; Haselmair, Ruth; Kuhn, Elisabeth; Schunko, Christoph; Vogl, Christian R

    2012-11-16

    In ethnobotanical research, the investigation into traditional knowledge of medicinal plants in the context of migration has been of increasing interest in recent decades since it is influenced and changed by new environmental and social conditions. It most likely undergoes transformation processes to match the different living circumstances in the new location. This study compares the traditional knowledge of medicinal plants held by Tyroleans - and their descendants - who emigrated to Australia, Brazil and Peru at different time scales. The study's findings allow a discussion of the complexities and dynamics that influence this knowledge within the context of long-distance migration. Information was obtained from 65 informants by free-listing, semi-structured interviews and non-participatory observation in Tyrol (Austria) and the migrants' countries: Australia, Brazil and Peru. The collected data was analysed using different quantitative approaches, including statistical tests, and compared between the countries of investigation. All respondents in all four investigation areas claimed that they had knowledge and made use of medicinal plants to treat basic ailments in their day-to-day lives. Informants made 1,139 citations of medicinal plants in total in free lists, which correspond to 164 botanical taxa (genus or species level) in Tyrol, 87 in Australia, 84 in Brazil and 134 in Peru. Of all the botanical taxa listed, only five (1.1%) were listed in all four countries under investigation. Agreement among informants within free lists was highest in Tyrol (17%), followed by Peru (12.2%), Australia (11.9%) and Brazil (11.2%). The proportion of agreement differs significantly between informants in Australia and Tyrol (p = 0.001), Brazil and Tyrol (p = 0.001) and Peru and Tyrol (p = 0.001) and is similar between informants in the migrant countries, as indicated by statistical tests. We recorded 1,286 use citations according to 744 different uses (Tyrol: 552, Australia

  14. Plant-wide modelling of phosphorus transformations in wastewater treatment systems: Impacts of control and operational strategies.

    Science.gov (United States)

    Solon, K; Flores-Alsina, X; Kazadi Mbamba, C; Ikumi, D; Volcke, E I P; Vaneeckhaute, C; Ekama, G; Vanrolleghem, P A; Batstone, D J; Gernaey, K V; Jeppsson, U

    2017-04-15

    The objective of this paper is to report the effects that control/operational strategies may have on plant-wide phosphorus (P) transformations in wastewater treatment plants (WWTP). The development of a new set of biological (activated sludge, anaerobic digestion), physico-chemical (aqueous phase, precipitation, mass transfer) process models and model interfaces (between water and sludge line) were required to describe the required tri-phasic (gas, liquid, solid) compound transformations and the close interlinks between the P and the sulfur (S) and iron (Fe) cycles. A modified version of the Benchmark Simulation Model No. 2 (BSM2) (open loop) is used as test platform upon which three different operational alternatives (A1, A2, A3) are evaluated. Rigorous sensor and actuator models are also included in order to reproduce realistic control actions. Model-based analysis shows that the combination of an ammonium ( [Formula: see text] ) and total suspended solids (XTSS) control strategy (A1) better adapts the system to influent dynamics, improves phosphate [Formula: see text] accumulation by phosphorus accumulating organisms (XPAO) (41%), increases nitrification/denitrification efficiency (18%) and reduces aeration energy (Eaeration) (21%). The addition of iron ( [Formula: see text] ) for chemical P removal (A2) promotes the formation of ferric oxides (XHFO-H, XHFO-L), phosphate adsorption (XHFO-H,P, XHFO-L,P), co-precipitation (XHFO-H,P,old, XHFO-L,P,old) and consequently reduces the P levels in the effluent (from 2.8 to 0.9 g P.m(-3)). This also has an impact on the sludge line, with hydrogen sulfide production ( [Formula: see text] ) reduced (36%) due to iron sulfide (XFeS) precipitation. As a consequence, there is also a slightly higher energy production (Eproduction) from biogas. Lastly, the inclusion of a stripping and crystallization unit (A3) for P recovery reduces the quantity of P in the anaerobic digester supernatant returning to the water line and allows

  15. Production of herbicide-resistant coffee plants (Coffea canephora P. via Agrobacterium tumefaciens-mediated transformation

    Directory of Open Access Journals (Sweden)

    Alessandra Ferreira Ribas

    2006-01-01

    Full Text Available Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supplemented with 5µM N6 - (2-isopentenyl-adenine (2-iP. Ten µM ammonium glufosinate was used for putative transgenic somatic embryos selection. Presence and integration of the bar gene were confirmed by PCR and Southern blot analysis. Selected transgenic coffee plants sprayed with up to 1600 mg.L-1 of FinaleTM, a herbicide containing glufosinate as the active ingredient, retained their pigmentation and continued to grow normally during ex vitro acclimation.Plantas transgênicas de Coffea canephora P resistentes ao herbicida glufosinato de amônio foram regeneradas a partir de explantes foliares co-cultivados com Agrobacterium tumefaciens EHA105 contendo o plasmídio pCambia3301 que contém os genes bar e uidA ambos sob controle do promotor 35S. Embriogênese somática direta foi induzida no meio contendo ¼ da concentração de macro, metade da concentração de micronutrientes do meio MS, constituintes orgânicos do meio B5 e 30 g.L-1 de sacarose suplementado com 5µM N6 - (2-isopentenil-adenina (2-iP e 10 µM de glufosinato de amônio para seleção de embriões transgênicos putativos. A presença e a integração do gene bar foram confirmados pelas análises de PCR e Southern blot. As plantas transgênicas selecionadas de café, pulverizadas com 1600 mg.L-1 do herbicida FinaleTM que contém glufosinato como ingrediente ativo, mantiveram a coloração e continuaram crescendo normalmente na aclimatação ex vitro.

  16. Development of an efficient transformation method by Agrobacterium tumefaciens and high throughput spray assay to identify transgenic plants for woodland strawberry (Fragaria vesca) using NPTII selection.

    Science.gov (United States)

    Pantazis, Christopher J; Fisk, Sarah; Mills, Kerri; Flinn, Barry S; Shulaev, Vladimir; Veilleux, Richard E; Dan, Yinghui

    2013-03-01

    KEY MESSAGE : We developed an efficient Agrobacterium -mediated transformation method using an Ac/Ds transposon tagging construct for F. vesca and high throughput paromomycin spray assay to identify its transformants for strawberry functional genomics. Genomic resources for Rosaceae species are now readily available, including the Fragaria vesca genome, EST sequences, markers, linkage maps, and physical maps. The Rosaceae Genomic Executive Committee has promoted strawberry as a translational genomics model due to its unique biological features and transformability for fruit trait improvement. Our overall research goal is to use functional genomic and metabolic approaches to pursue high throughput gene discovery in the diploid woodland strawberry. F. vesca offers several advantages of a fleshy fruit typical of most fruit crops, short life cycle (seed to seed in 12-16 weeks), small genome size (206 Mbb/C), small plant size, self-compatibility, and many seeds per plant. We have developed an efficient Agrobacterium tumefaciens-mediated strawberry transformation method using kanamycin selection, and high throughput paromomycin spray assay to efficiently identify transgenic strawberry plants. Using our kanamycin transformation method, we were able to produce up to 98 independent kanamycin resistant insertional mutant lines using a T-DNA construct carrying an Ac/Ds transposon Launchpad system from a single transformation experiment involving inoculation of 22 leaf explants of F. vesca accession 551572 within approx. 11 weeks (from inoculation to soil). Transgenic plants with 1-2 copies of a transgene were confirmed by Southern blot analysis. Using our paromomycin spray assay, transgenic F. vesca plants were rapidly identified within 10 days after spraying.

  17. [Genetic transformation of buckwheat ( Fagopyrum esculentum Moench ) with AtNHX1 gene and regeneration of salt-tolerant transgenic plants].

    Science.gov (United States)

    Cheng, Li-Hong; Zhang, Bo; Xu, Zi-Qin

    2007-01-01

    The Arabidopsis thaliana tonoplast Na+ /H+ antiporter gene, AtNHX1, was transferred into buckwheat by Agrobacterium-mediated method. Transgenic buckwheat plants were regenerated and selected on MS basal medium supplemented with 2.0mg/L 6-BA, 1.0mg/L KT, 0.lmg/L IAA, 50mg/L kanamycin and 500mg/L carbenicillin. 426 seedlings from 36 resistant calli originated from 864 explants (transformed about at 4.17 percentage) exhibited resistance to kanamycin. The transformants were confirmed by PCR, Southern blotting, RT-PCR and Northern blotting analysis. After stress treatment for 6 weeks with 200mmol/L NaCl, transgenic plants survived, while wild-type plants did not. After 3 days of stress treatment through different concentrations of NaCl, transgenic plants accumulated higher concentration of Na+ and proline than the control plants. However, the K+ concentration of transgenic plants declined in comparison with the control plants. Moreover, the rutin content of the roots, stems and leaves of transgenic buckwheat increased than those of the control plants. These results showed that it could be possible to improve the salt-tolerance of crops with genetic technology.

  18. Assessing transformational change from institutionalising digital capabilities on implementation and development of Product-Service Systems: Learnings from the maritime industry

    DEFF Research Database (Denmark)

    Pagoropoulos, Aris; Maier, Anja; McAloone, Tim C.

    2017-01-01

    Digitization is rapidly reshaping industries and economic sectors. It enables novel Product-Service Systems (PSS) that transform customer/supplier relationships and introduces new value propositions. However, while opportunities for novel types of PSS arise, it is not clear how digitization...

  19. Agrobacterium-transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer.

    Science.gov (United States)

    Cheng, X; Sardana, R; Kaplan, H; Altosaar, I

    1998-03-17

    Over 2,600 transgenic rice plants in nine strains were regenerated from >500 independently selected hygromycin-resistant calli after Agrobacterium-mediated transformation. The plants were transformed with fully modified (plant codon optimized) versions of two synthetic cryIA(b) and cryIA(c) coding sequences from Bacillus thuringiensis as well as the hph and gus genes, coding for hygromycin phosphotransferase and beta-glucuronidase, respectively. These sequences were placed under control of the maize ubiquitin promoter, the CaMV35S promoter, and the Brassica Bp10 gene promoter to achieve high and tissue-specific expression of the lepidopteran-specific delta-endotoxins. The integration, expression, and inheritance of these genes were demonstrated in R0 and R1 generations by Southern, Northern, and Western analyses and by other techniques. Accumulation of high levels (up to 3% of soluble proteins) of CryIA(b) and CryIA(c) proteins was detected in R0 plants. Bioassays with R1 transgenic plants indicated that the transgenic plants were highly toxic to two major rice insect pests, striped stem borer (Chilo suppressalis) and yellow stem borer (Scirpophaga incertulas), with mortalities of 97-100% within 5 days after infestation, thus offering a potential for effective insect resistance in transgenic rice plants.

  20. An in vivo Transient Expression System Can Be Applied for Rapid and Effective Selection of Artificial MicroRNA Constructs for Plant Stable Genetic Transformation

    Institute of Scientific and Technical Information of China (English)

    Basdeo Bhagwat; Ming Chi; Li Su; Haifeng Tang; Guiliang Tang; Yu Xiang

    2013-01-01

    The utility of artificial microRNAs (amiRNAs) to induce loss of gene function has been reported for many plant species,but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable.In this study,expressions of amiRNAs through the gene backbone of Arabidopsis miR 168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation.A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments.Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs.This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.

  1. Electric power plant technology. Generators, transformers, auxiliary installations, protective systems. Lecture notes. 5. rev. ed.; Elektrische Kraftwerkstechnik. Generatoren, Transformatoren, Eigenbedarfsanlagen, Schutzeinrichtungen. Vorlesungsskript

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, Ulrich

    2009-07-01

    The lecture series on electric power plant engineering was initiated in 1969 by Prof. Helmut Schaefer, founder of the then department of power economy and power plant engineering. The lecture series was continuously updated in its contents and didactic approach, e.g. by integrating innovative power generation concepts like micro cogeneration plants and fuel cell systems. The focus is on the fundamentals and function of generators, main transformers, auxiliary installations and protective systems in power plant. Apart from the electrotechnical aspects, also aspects of mechanical engineering are gone into. Also discussed are energy conversion concepts fo renewable power generation plants like wind turbines and PV systems. This is the 5th, completely revised edition. It addresses primarily attendants of the lectures but may also serve as a textbook and reference manual for interested experts.

  2. Transformation of marine sediment to paddy soil: Primary marine, lacustrine, and land plant lipids

    Science.gov (United States)

    Mueller-Niggemann, Cornelia; Cao, Zhihong; Schwark, Lorenz

    2010-05-01

    More than fifty percent of the world's population feeds on rice. The continuous population increase and urban sprawl leads to an ever-increasing demand for new rice cultivation area, in particular China. For centuries suitable coastal areas in China have been exploited for land reclamation, i.e. conversion of coastal marine and lacustrine marshlands into rice paddy fields. Flooded rice paddies are considered one of the major biogenic sources of methane into the atmospheric. Methane is thought to be about 30 times more efficient as greenhouse gas, when compared to carbon dioxide. Overall, rice fields are assumed to contribute app. 10-25% to global CH4 production. It is thus paramount importance to study the effects of increasing rice cultivation and land reclamation in China. For global carbon cycle investigation, it is crucial whether paddy soils, due to their large extent and higher carbon turnover, serve as carbon (CO2) sinks or sources. Here we present results from a chronosequence study of paddy soils with different and well known starting dates of cultivation, in the Zhejiang province (Yangtze River delta) by land reclamation through the building of protective dikes over the past 2000 years. Two end members of natural sediments subjected to land reclamation, a marine tidal mudflat in the Yangtze delta and a coastal lake, represent the substrate on which the paddy soil evolution started. Dike systems were constructed 2000, 1000, 700, 300, 100, and 50 years before present. We are thus able to follow the evolution of rice paddy soils developed on marine sediments using eight well defined tie-points. This chronosequence is then used for assessing the relative proportion of primary marine or lacustrine organic matter preserved in present day soils and to identify the amount and composition of organic matter added since cultivation started. Paddy soil management introduces rice plants debris and exudates as well as rice-associated microbial biomass (covered in a

  3. Using an extractive Fourier transform infrared spectrometer for improving cleanroom air quality in a semiconductor manufacturing plant.

    Science.gov (United States)

    Li, Shou-Nan; Chang, Chin-Ta; Shih, Hui-Ya; Tang, Andy; Li, Alen; Chen, Yin-Yung

    2003-01-01

    A mobile extractive Fourier transform infrared (FTIR) spectrometer was successfully used to locate, identify, and quantify the "odor" sources inside the cleanroom of a semiconductor manufacturing plant. It was found that ozone (O(3)) gas with a peak concentration of 120 ppm was unexpectedly releasing from a headspace of a drain for transporting used ozonized water and that silicon tetrafluoride (SiF(4)) with a peak concentration of 3 ppm was off-gassed from silicon wafers after dry-etching processing. When the sources of the odors was pinpointed by the FTIR, engineering control measures were applied. For O(3) control, a water-sealed pipeline was added to prevent the O(3) gas (emitting from the ozonized water) from entering the mixing unit. A ventilation system also was applied to the mixing unit in case of O(3) release. For SiF(4) mitigation, before the wafer-out chamber was opened, N(2) gas with a flow rate of 150 L/min was used for 100 sec to purge the wafer-out chamber, and a vacuum system was simultaneously activated to pump away the purging N(2). The effectiveness of the control measures was assured by using the FTIR. In addition, the FTIR was used to monitor the potential hazardous gas emissions during preventative maintenance of the semiconductor manufacturing equipment.

  4. Assessment of nitrous oxide emission from cement plants: real data measured with both Fourier transform infrared and nondispersive infrared techniques.

    Science.gov (United States)

    Mosca, Silvia; Benedetti, Paolo; Guerriero, Ettore; Rotatori, Mauro

    2014-11-01

    Nitrous oxide (N2O) is the third most important greenhouse gas after carbon dioxide and methane, and contributes about 6% to the greenhouse effect. Nitrous oxide is a minor component of the atmosphere, and it is a thousand times less than carbon dioxide (CO2). Nevertheless, it is much more potent than CO2 and methane, owing to its long stay in the atmosphere of approximately 120 yr and the high global warmingpotential (GWP) of298 times that of CO2. Although greenhouse gases are natural in the atmosphere, human activities have changed the atmospheric concentrations. Most of the values of emission of nitrous oxide are still obtained by means ofemission factors and not actually measured; the lack ofreal data may result in an underestimation ofcurrent emissions. The emission factors used for the calculation of N2O can be obtained from the "Guidelines for the implementation of the national inventory of emissions" of the Intergovernmental Panel on Climate Change, which refer to all nations for the realization of their inventory. This study will present real data, measured in several Italian cement plants with different characteristics. The work also shows a comparison between N2O concentration measured with in situ-Fourier transform IR (FTIR) and the reference method EN ISO 21258 based on nondispersive IR (NDIR), in order to investigate the interfering compounds in the measurement with NDIR.

  5. Monitoring the spread of recombinant DNA from field plots with transgenic sugar beet plants by PCR and natural transformation of Pseudomonas stutzeri.

    Science.gov (United States)

    Meier, Petra; Wackernagel, Wilfried

    2003-06-01

    Previous studies had shown that recombinant DNA can be detected for several months in soil after the deposition of litter from transgenic (tg) plants. Here we show by PCR monitoring of field releases of tg sugar beet plants that during the growth of the plants the soil close to the plants and also plant material contains recombinant DNA, in the form of extracellular molecules. Surprisingly, the monitoring also revealed the presence of tg DNA in many field plots (30-70%) in which tg plants were never grown. These studies and the further monitoring during other tg sugar beet release experiments by PCR and a novel bioassay (measuring the transforming potential of recombinant DNA for Pseudomonas stutzeri) indicated that recombinant DNA was only detectable in the surface soil of field plots and their vicinity where flowering of the tg beet plants was allowed. Recombinant DNA was found in soil at a distance of 50 m from pollen-producing plants surrounded by a strip with hemp plants as a containment regime. It is concluded that recombinant DNA is deposited in soil during the growth of tg sugar beets and that a major mechanism of recombinant DNA spread in the environment is the dispersal of pollen which allows recombinant DNA to persist in the field plot for at least a year.

  6. [Construction of plant expression vectors with PMI gene as selection marker and their utilization in transformation of Salvia miltiorrhiza f. alba].

    Science.gov (United States)

    Tao, Ru; Zhang, You-Can; Fang, Qian; Shi, Ren-Jiu; Li, Yan-Ling; Huang, Lu-Qi; Hao, Gang-Ping

    2014-04-01

    To construct plant expression pCAMBIA1301-PMI by substituting PMI for hygromycin resistance gene in pCAMBIA1301 and obtain transgenic Salvia miltiorrhiza f. alba using PMI-mannose selection system. The 6-phosphomannose isomerase gene (PMI) of Escherichia coli was amplified by PCR. Sequence analysis showed that it shared 100% amino acids identities with the sequences of PMI genes isolates reported in the NCBI. Based on pCAMBIA1305, the plant expression pCAMBIA1305-PMI was constructed successfully by substituting PMI for hygromycin resistance gene in pCAMBIA1305. pCAMBIA1305-PMI was transformed into Agrobacterium tumefaciens LBA4404, and then the leaves of S. miltiorrhiza f. alba were inoculated in LBA4404 with pCAMBIA1305-PMI. Plant expression pCAMBIA1301-PMI was successfully constructed and the leaves of S. miltiorrhiza f. alba inoculated in LBA4404 with pCAMBIA1305-PMI were selected on medium supplemented with a combination of 20 g x L(-1) mannose and 10 g x L(-1) sucrose as a carbon source. The transformation efficiency rate was 23.7%. Genetic transformation was confirmed by PCR, indicating that a new method for obtaining transgenic S. miltiorrhiza f. alba plants was developed using PMI-mannose selection system.

  7. Repression of fungal plant pathogens and fungal-related contaminants: Selected ecosystem services by soil fauna communities in agroecosystems

    Science.gov (United States)

    Meyer-Wolfarth, Friederike; Schrader, Stefan; Oldenburg, Elisabeth; Brunotte, Joachim; Weinert, Joachim

    2017-04-01

    In agroecosystems soil-borne fungal plant diseases are major yield-limiting factors which are difficult to control. Fungal plant pathogens, like Fusarium species, survive as a saprophyte in infected tissue like crop residues and endanger the health of the following crop by increasing the infection risk for specific plant diseases. In infected plant organs, these pathogens are able to produce mycotoxins. Mycotoxins like deoxynivalenol (DON) persist during storage, are heat resistant and of major concern for human and animal health after consumption of contaminated food and feed, respectively. Among fungivorous soil organisms, there are representatives of the soil fauna which are obviously antagonistic to a Fusarium infection and the contamination with mycotoxins. Specific members of the soil macro-, meso-, and microfauna provide a wide range of ecosystem services including the stimulation of decomposition processes which may result in the regulation of plant pathogens and the degradation of environmental contaminants. Investigations under laboratory conditions and in field were conducted to assess the functional linkage between soil faunal communities and plant pathogenic fungi (Fusarium culmorum). The aim was to examine if Fusarium biomass and the content of its mycotoxin DON decrease substantially in the presence of soil fauna (earthworms: Lumbricus terrestris, collembolans: Folsomia candida and nematodes: Aphelenchoides saprophilus) in a commercial cropping system managed with conservation tillage located in Northern Germany. The results of our investigations pointed out that the degradation performance of the introduced soil fauna must be considered as an important contribution to the biodegradation of fungal plant diseases and fungal-related contaminants. Different size classes within functional groups and the traits of keystone species appear to be significant for soil function and the provision of ecosystem services as in particular L. terrestris revealed to

  8. Effects of invasive plant species on pollinator service and reproduction in native plants at Acadia National Park

    Science.gov (United States)

    Stubbs, C.J.; Drummond, F.; Ginsberg, H.

    2007-01-01

    Invasive plant species can have profound negative effects on natural communities by competively excluding native species. Berberis thunbergii (Japanese barberry), Frangula alnus (glossy or alder buckthorn) and Lythrum salicaria (purple loosestrife) are invasive species known to reduce native plant diversity and are thus of great concern to Acadia National Park. Pollinators visit them for nectar and pollen. The effects of invasive plant species on pollinator behavior were investigated by comparing pollinator visitation to co-flowering native and invasive species with visitation to native species growing alone. The effect of invasives on pollination of native plants was studied by comparing fruit set in patches of the native species growing near invasives with patches far from invasive species in Acadia National Park. The coflowering pairs were as follows: in the spring native Vaccinium angustifolium (lowbush blueberry) was paired with B. thunbergii; in early summer native Viburnum nudum (wild raisin) was paired with F. alnus ; in late summer native Spiraea alba (meadowsweet) was paired with L. salicaria. We investigated whether these invasives competed with native plants for pollinators in Acadia and thus negatively affected native plant reproduction. Our objectives were to determine: 1) the influence, if any, of each invasive on pollinator visitation to a co-flowering native species, 2) factors that might affect visitation, 3) invasive pollen transfer to native plants, and 4) whether invasives influence native plant reproduction (fruit set). Our findings indicate that at times the number of flower visitors to natives was lower or the species composition of visitors different when invasives were present, that invasives sometimes attracted more pollinators, that generally the invasives were more rewarding as far as nectar and pollen availability for pollinators, and that generally native plant fruit set and seed set was not significantly lowered in the presence of

  9. A GPU-based real time high performance computing service in a fast plant system controller prototype for ITER

    Energy Technology Data Exchange (ETDEWEB)

    Nieto, J., E-mail: jnieto@sec.upm.es [Grupo de Investigacion en Instrumentacion y Acustica Aplicada. Universidad Politecnica de Madrid, Crta. Valencia Km-7, Madrid 28031 Spain (Spain); Arcas, G. de; Ruiz, M. [Grupo de Investigacion en Instrumentacion y Acustica Aplicada. Universidad Politecnica de Madrid, Crta. Valencia Km-7, Madrid 28031 Spain (Spain); Vega, J. [Asociacion EURATOM/CIEMAT para Fusion, Madrid (Spain); Lopez, J.M.; Barrera, E. [Grupo de Investigacion en Instrumentacion y Acustica Aplicada. Universidad Politecnica de Madrid, Crta. Valencia Km-7, Madrid 28031 Spain (Spain); Castro, R. [Asociacion EURATOM/CIEMAT para Fusion, Madrid (Spain); Sanz, D. [Grupo de Investigacion en Instrumentacion y Acustica Aplicada. Universidad Politecnica de Madrid, Crta. Valencia Km-7, Madrid 28031 Spain (Spain); Utzel, N.; Makijarvi, P.; Zabeo, L. [ITER Organization, CS 90 046, 13067 St. Paul lez Durance Cedex (France)

    2012-12-15

    Highlights: Black-Right-Pointing-Pointer Implementation of fast plant system controller (FPSC) for ITER CODAC. Black-Right-Pointing-Pointer GPU-based real time high performance computing service. Black-Right-Pointing-Pointer Performance evaluation with respect to other solutions based in multi-core processors. - Abstract: EURATOM/CIEMAT and the Technical University of Madrid UPM are involved in the development of a FPSC (fast plant system control) prototype for ITER based on PXIe form factor. The FPSC architecture includes a GPU-based real time high performance computing service which has been integrated under EPICS (experimental physics and industrial control system). In this work we present the design of this service and its performance evaluation with respect to other solutions based in multi-core processors. Plasma pre-processing algorithms, illustrative of the type of tasks that could be required for both control and diagnostics, are used during the performance evaluation.

  10. Technological innovation and convergent journalism. Case study on the transformation process of Bavaria’s public broadcasting service

    Directory of Open Access Journals (Sweden)

    Klaus MEIER

    2017-01-01

    Full Text Available As a consequence of technological innovations and their social utilisation, media organisations and newsrooms are undergoing a fundamental transformation process. This article analyses the interrelations between technological innovations and the challenges of convergent journalism. Studies conducted on newsroom convergence to date set out from one-sided influences: They investigated the extent to which technical innovations determine the newsrooms, or whether journalistic structures and long-range trends are so strong as to largely obstruct the adoption of new technologies in journalism. Therefore technology — especially in studies carried out on newsroom convergence — is considered as an influencing factor on somehow resisting or non-resisting newsrooms. We broaden the scope of this investigation by considering that any such influence might not come exclusively from technology, but that impulses can come also from innovative newsrooms: technological gaps, wishes and obstacles are identified from the journalist's viewpoint and brought to the attention of the developers of newsroom technologies — on the basis of empirical research findings from a case study of Bavaria’s public broadcasting service, Bayerischer Rundfunk (BR. With its 5.000 employees, BR is one of Germany's largest media organisations. It is undergoing a fundamental and long-term convergence process. A variety of gaps, problems and wishes came to light from 25 in-depth interviews with journalists working on innovative projects. For example, criticism is expressed of complicated, partly unknown or non-existent networking of cross-media production systems. There is a desire for the increased use of consumer devices like smartphones in professional news production. There exists a creative potential for innovative solutions, but these are not comprehensively evaluated. To date, there has been no systematic implementation of the findings of innovative media projects in everyday

  11. Agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf midribs as explants in ramie [Boehmeria nivea (L.) Gaud].

    Science.gov (United States)

    An, Xia; Wang, Bo; Liu, Lijun; Jiang, Hui; Chen, Jie; Ye, Shengtuo; Chen, Leiyu; Guo, Pingan; Huang, Xing; Peng, Dingxiang

    2014-05-01

    In this study, leaf midribs, the elite explants, were used for the first time to develop an efficient regeneration and transformation protocol for ramie [Boehmeria nivea (L.) Gaud.] via Agrobacterium-mediated genetic transformation. Sensitivity of leaf midribs regeneration to kanamycin was evaluated, which showed that 40 mg l(-1) was the optimal concentration needed to create the necessary selection pressure. Factors affecting the ramie transformation efficiency were evaluated, including leaf age, Agrobacterium concentration, length of infection time for the Agrobacterium solution, acetosyringone concentration in the co-cultivation medium, and the co-cultivation period. The midrib explants from 40-day-old in vitro shoots, an Agrobacterium concentration at OD600 of 0.6, 10-min immersion in the bacteria solution, an acetosyringone concentration of 50 mg l(-1) in the co-cultivation medium and a 3-day co-cultivation period produced the highest efficiencies of regeneration and transformation. In this study, the average transformation rate was 23.25%. Polymerase chain reactions using GUS and NPTII gene-specific primers, Southern blot and histochemical GUS staining analyses further confirmed that the transgene was integrated into the ramie genome and expressed in the transgenic ramie. The establishment of this system of Agrobacterium-mediated genetic transformation and regeneration of transgenic plants will be used not only to introduce genes of interest into the ramie genome for the purpose of trait improvement, but also as a common means of testing gene function by enhancing or inhibiting the expression of target genes.

  12. Investigating the formation and toxicity of nitrogen transformation products of diclofenac and sulfamethoxazole in wastewater treatment plants

    Energy Technology Data Exchange (ETDEWEB)

    Osorio, Victoria; Sanchís, Josep [Water and Soil Quality Research Group, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona (Spain); Abad, Jose Luís [RUBAM-IIQAC-CSIC, Jordi Girona 18-26, Barcelona (Spain); Ginebreda, Antoni; Farré, Marinella [Water and Soil Quality Research Group, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona (Spain); Pérez, Sandra, E-mail: spsqam@idaea.csic.es [Water and Soil Quality Research Group, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona (Spain); Barceló, Damià [Water and Soil Quality Research Group, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona (Spain); Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, Emili Grahit 101, Girona (Spain)

    2016-05-15

    Highlights: • Environmental monitoring of recently discovered TPs of diclofenac in wastewater and surface water. • Quantitative determination of non-detected TPs of sulfamethoxazole in wastewater and impacted surface waters. • Toxicity assessment of diclofenac and its TPs in a panel of standard assays of aquatic organism. • Only nitro-diclofenac proved to be more toxic than the parent compound. - Abstract: Diclofenac (DCF) and sulfamethoxazole (SMX) are highly consumed pharmaceuticals and concentrated in effluents from conventional wastewater treatment plants (WWTPs) since they are not completely eliminated. Under microbial mediated nitrification/denitrification processes occurring in nitrifying activated sludge DCF biotransformed into its nitroso and nitro derivatives (NO-DCF and NO{sub 2}-DCF, respectively). SMX was biotransformed under denitrification conditions in water/sediment batch reactors into its nitro and desamino derivatives (NO{sub 2}-SMX and Des-SMX, respectively). Four transformation products (TPs) from DCF and SMX were analized in wastewaters (WW) and receiving surface waters (SW). Nitrifying/denitrifying-derivatives of DCF and SMX were detected for the first time in WW and SW at one order of magnitude lower than their parent compounds. Relationships observed among levels of NO-DCF, NO{sub 2}-DCF and nitrogen-species tentatively suggested that nitrification/denitrification processes are involved in nitration and nitrosation of DCF during biological WW treatment. Acute toxicity of analytes to Daphnia magna and Vibrio fischeri was assessed individually and in mixtures with other relevant micropollutants. Individual effects showed these compounds as not harmful and not toxic. However, synergism effects observed in mixtures evidenced that contribution of these compounds to overall toxicity of complex environmental samples, should not be dismissed.

  13. Implications for Ecosystem Services of Watershed Processes that affect the Transport and Transformations of Mercury in an Adirondack Stream Basin

    Science.gov (United States)

    Burns, D. A.; Riva-Murray, K.; Bradley, P. M.

    2012-12-01

    Mercury (Hg) is a potent neurotoxin that can affect the health of humans and wildlife through the ingestion of methyl Hg. Mercury contamination of ecosystems originates from human activities such as mining, coal burning and other industrial emissions, and the use of Hg-containing products. Natural sources such as volcanic and geothermal emissions and the weathering of Hg-bearing minerals also contribute to Hg contamination, but are believed to be minor sources in most ecosystems. Various ecosystem disturbances including fires, forest harvesting, and the submergence of land by impoundment may also contribute to Hg ecosystem contamination by mobilizing stores that have previously originated from the sources described above. Mercury from a mix of regional and global emissions sources is transported in the atmosphere to remote landscapes that are distant from local emissions sources. The Adirondacks of New York State is a forested, mountainous region characterized by abundant lakes and streams, and is distant from local emissions sources. Recreational fishing, wildlife viewing, hiking, and hunting are valued ecosystem services in this region. Here, we report on the relevance to ecosystem services of findings based on five years of Hg data collection of stream water, groundwater, invertebrates, and fish in the upper Hudson River basin in the central part of the Adirondack region. The New York State Dept. of Health has issued fish consumption advisories for the entire Adirondacks based on elevated levels previously measured in lakes and rivers of this region. Our work seeks improved understanding and models of the landscape sources and watershed processes that control the transformation of Hg to its methyl form (MeHg), the transport of MeHg to streams, and bioaccumulation of MeHg in aquatic food webs. Mean annual atmospheric Hg deposition was 6.3 μg/m2/yr during 2007-09, compared to mean annual filtered total Hg stream yields of 1.66 μg/m2/yr and filtered MeHg stream

  14. 'Not just methadone Tracy': transformations in service-user identity following the introduction of hepatitis C treatment into Australian opiate substitution settings.

    Science.gov (United States)

    Rance, Jake; Treloar, Carla

    2014-03-01

    To explore identity transformation among service users attending opiate substitution therapy (OST) clinics following the introduction of hepatitis C (HCV) care and treatment. An interview-based substudy of the Australian ETHOS (Enhancing Treatment for Hepatitis C in Opiate Substitution Settings) project. Three OST clinics and one community health centre (operating a public OST) in New South Wales, Australia. Participants were interviewed at the recruitment sites. The sample consisted of 57 OST service users concurrently living with HCV, 16 staff, including specialist HCV clinicians, and three peer-support workers, employed on the ETHOS project. Semi-structured interviews. Service-user participants largely welcomed the introduction of HCV treatment as a practical, clinical intervention that also intimated a more comprehensive, holistic form of care. Negative stereotypes characteristic of OST settings-of limited, routinized clinical exchanges and minimal social-care interaction-were unsettled, opening up the possibility of new relations between staff and service users. The shift in the dynamic of the clinical encounter to address health in addition to dependence appeared to catalyse transformative possibilities not only for the therapeutic alliance but also for service-user understandings of self and identity. Trial introduction of HCV care and treatment in selected Australian opiate substitution therapy (OST) clinics may have facilitated alternative, 'non-addict' identities to emerge from a clinical setting where the stigmatizing figure of 'the drug user' has traditionally prevailed.

  15. Studies of transformational leadership in the consumer service workgroup: cooperative conflict resolution and the mediating roles of job satisfaction and change commitment.

    Science.gov (United States)

    Yang, Yi-Feng

    2012-10-01

    The present paper evaluates the effect of transformational leadership on job satisfaction and change commitment along with their interconnected effects (mediation) on cooperative conflict resolution (management) in customer service activities in Taiwan. The multi-source samples consist of data from personnel serving at customer centers (workgroups), such as phone service personnel, customer representatives, financial specialists, and front-line salespeople. An empirical study was carried out using a multiple mediation procedure incorporating boot-strapping techniques and PRODCLIN2 with structural equation modeling (SEM) analysis. The results indicate that the main effect of the leadership style on cooperative conflict resolution is mediated by change commitment and job satisfaction.

  16. 转型时代的图书馆服务与体验%Library service and the experience in transformation age

    Institute of Scientific and Technical Information of China (English)

    陈抒; 康健; 徐青

    2015-01-01

    数字时代的来临使得图书馆也必须不断的创新自身的经营模式,从而带给民众更好的服务与体验。在这种背景下,本文分别探析了转型时代的图书馆价值、转型时代的图书馆服务与体验发展,并进行了总结,给出了发展建议。%The advent of the digital age makes the library also must constantly innovate their business model, so as to bring people to a better service and experience. In this context, this paper discusses the transformation of the era of value, the transformation of the era of library service and development experience, and summarized, the development Suggestions are given.

  17. Savannah River Plant engineering and design history. Volume 4: 300/700 Areas & general services and facilities

    Energy Technology Data Exchange (ETDEWEB)

    1957-01-01

    The primary function of the 300 Area is the production and preparation of the fuel and target elements required for the 100 Area production reactors. Uranium slugs and lithium-aluminium alloy control and blanket rods are prepared in separate structures. Other facilities include a test pile, a physics assembly laboratory, an office and change house, an electrical substation, and various service facilities such as rail lines, roads, sewers, steam and water distribution lines, etc. The 700 Area contains housing and facilities for plant management, general plant services, and certain technical activities. The technical buildings include the Main Technical Laboratory, the Waste Concentration Building, the Health Physics Headquarters, and the Health Physics Calibration building. Sections of this report describe the following: development of the 300-M Area; selection and description of process; design of main facilities of the 300 Area; development of the 700-A Area; design of the main facilities of the 700 Area; and general services and facilities, including transportation, plant protection, waste disposal and drainage, site work, pilot plants, storage, and furniture and fixtures.

  18. Constitutive expression of the tzs gene from Agrobacterium tumefaciens virG mutant strains is responsible for improved transgenic plant regeneration in cotton meristem transformation.

    Science.gov (United States)

    Ye, Xudong; Chen, Yurong; Wan, Yuechun; Hong, Yun-Jeong; Ruebelt, Martin C; Gilbertson, Larry A

    2016-03-01

    KEY MESSAGE : virG mutant strains of a nopaline type of Agrobacterium tumefaciens increase the transformation frequency in cotton meristem transformation. Constitutive cytokinin expression from the tzs gene in the virG mutant strains is responsible for the improvement. Strains of Agrobacterium tumefaciens were tested for their ability to improve cotton meristem transformation frequency. Two disarmed A. tumefaciens nopaline strains with either a virGN54D constitutively active mutation or virGI77V hypersensitive induction mutation significantly increased the transformation frequency in a cotton meristem transformation system. The virG mutant strains resulted in greener explants after three days of co-culture in the presence of light, which could be attributed to a cytokinin effect of the mutants. A tzs knockout strain of virGI77V mutant showed more elongated, less green explants and decreased cotton transformation frequency, as compared to a wild type parental strain, suggesting that expression of the tzs gene is required for transformation frequency improvement in cotton meristem transformation. In vitro cytokinin levels in culture media were tenfold higher in the virGN54D strain, and approximately 30-fold higher in the virGI77V strain, in the absence of acetosyringone induction, compared to the wild type strain. The cytokinin level in the virGN54D strain is further increased upon acetosyringone induction, while the cytokinin level in the virGI77V mutant is decreased by induction, suggesting that different tzs gene expression regulation mechanisms are present in the two virG mutant strains. Based on these data, we suggest that the increased cytokinin levels play a major role in increasing Agrobacterium attachment and stimulating localized division of the attached plant cells.

  19. Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid.

    Science.gov (United States)

    Zyprian, E; Kado, C I

    1990-08-01

    A new binary vector system for Agrobacterium-mediated plant transformation was developed. A set of four mini-T vectors comprised of T-DNA border sequences from nopaline-type Ti-plasmid pTiC58 flanking a chimaeric hygromycin-resistance gene for selection of transformants and up to eight unique restriction sites for cloning foreign DNA was constructed on a broad-host replicon containing the oriV of plasmid pSa. In two of the constructs these multiple cloning sites are flanked by a strong promoter to activate transcription of inserted DNA in planta. High-efficiency transformation was prompted by a high-copy, stable virulence helper plasmid pUCD2614, which contains a cloned virulence region of pTiC58 and tandem copies of the par locus of plasmid pTAR. Southern blot hybridization and genetic analyses of the progeny of transformed plants showed that the hygromycin resistance gene was stably inherited.

  20. Effect of water withdrawal on formation of free radical, proline accumulation and activities of antioxidant enzymes in ZAT12-transformed transgenic tomato plants.

    Science.gov (United States)

    Chandra Rai, Avinash; Singh, Major; Shah, Kavita

    2012-12-01

    Water stress often leads to the accumulation of reactive oxygen species (ROS) and their excessive production alters the activities of enzymes involved in their removal. ZAT12 is a member of stress-responsive C(2)H(2) type Zinc Finger Protein (ZFP) reported to control the expression of several stress-activated genes in plants through ROS signaling. The ZAT12-transformed tomato lines (cv. H-86 variety Kashi Vishesh) when subjected to water withdrawal for 7, 14 and 21 days revealed significant and consistent changes in activities of enzymes SOD, CAT, APX, GR and POD paralleled with an increased proline levels. Unlike that in wild-type tomato, the leaf superoxide anion and hydrogen peroxide concentrations in the transformed tomato plants did not alter much, suggesting a well regulated formation of free radicals suppressing oxidative stress in the latter. Results suggest BcZAT12-transformed tomato lines ZT1, ZT2 and ZT6 to be better adapted to drought stress tolerance by accumulation of osmolyte proline and increased antioxidant response triggered by the ZAT12 gene. Therefore, the ZAT12-transformed tomato cv. H-86 lines will prove useful for higher yield of tomato crop in regions affected with severe drought stress.

  1. Research progress in microbial transformation of plant secondary metabolite%微生物转化植物次生代谢产物研究进展

    Institute of Scientific and Technical Information of China (English)

    邹宇; 马堃; 尹冬梅

    2013-01-01

    植物次生代谢产物具有许多重要的生理功能,它的微生物转化成为近年来的研究热点.本文综述了植物次生代谢产物的种类和功能以及微生物转化植物次生代谢产物的类型和特点,展望了微生物转化技术在生物活性物质生产和医药保健品研发等领域的广阔应用前景.%The plant secondary metabolites has many important physiological functions and its microbial transformation has become a hot research topic in this field in recent years.In this paper,several species and function of plant secondary metabolite had been summarized.Meanwhile,various types and characteristics of microbial transformation of plant secondary metabolite had been introduced.Moreover,it was predicted that microbial transformation would have wide application prospect in production of bioactive substances and development of pharmaceuticals and health foods.

  2. Overview of activities in the U.S. related to continued service of nuclear power plant concrete structures

    Directory of Open Access Journals (Sweden)

    Naus D.J.

    2011-04-01

    Full Text Available Safety-related nuclear power plant concrete structures are described and commentary on continued service assessments of these structures is provided. In-service inspection and testing requirements in the U.S. are summarized. The license renewal process in the U.S. is outlined and its current status noted. A summary of operating experience related to U.S. nuclear power plant concrete structures is presented. Several candidate areas are identified where additional research would be of benefit to aging management of NPP concrete structures. Finally current ORNL activities related to aging-management of concrete structures are outlined: development of operating experience database, application of structural reliability theory, and compilation of elevated temperature concrete material property data and information.

  3. Studies of transformational leadership in consumer service: leadership trust and the mediating-moderating role of cooperative conflict management.

    Science.gov (United States)

    Yang, Yi-Feng

    2012-02-01

    This is the third in a series of studies evaluating how transformational leadership is associated with related variables such as job satisfaction, change commitment, leadership trust, cooperative conflict management, and market orientation. The present paper evaluates the effects of transformational leadership and cooperative conflict management along with their mediating and moderating of leadership trust in the life insurance industry for two sample groups, sales managers and sales employees. The main effect of leadership trust was mediated and moderated by cooperative conflict management. Cooperative conflict management made a more important contribution than transformational leadership or the moderating effect (interaction), but these three together were the most important variables predicting highest leadership trust. Transformational leadership has an indirect influence on leadership trust. This work summarizes the specific contribution and importance of building successful leadership trust associations with employees in relation to leadership and satisfaction with change commitment.

  4. Organelle transformation.

    Science.gov (United States)

    Bhattacharya, Anjanabha; Kumar, Anish; Desai, Nirali; Parikh, Seema

    2012-01-01

    The source of genetic information in a plant cell is contained in nucleus, plastids, and mitochondria. Organelle transformation is getting a lot of attention nowadays because of its superior performance over the conventional and most commonly used nuclear transformation for obtaining transgenic lines. Absence of gene silencing, strong predictable transgene expression, and its application in molecular pharming, both in pharmaceutical and nutraceuticals, are some of many advantages. Other important benefits of utilizing this technology include the absence of transgene flow, as organelles are maternally inherited. This may increase the acceptability of organelle transformation technology in the development of transgenic crops in a wider scale all over the globe. As the need for crop productivity and therapeutic compounds increases, organelle transformation may be able to bridge the gap, thereby having a definite promise for the future.

  5. The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species

    Directory of Open Access Journals (Sweden)

    von Arnim Albrecht G

    2009-05-01

    Full Text Available Abstract Background Plant genome sequencing has resulted in the identification of a large number of uncharacterized genes. To investigate these unknown gene functions, several transient transformation systems have been developed as quick and convenient alternatives to the lengthy transgenic assay. These transient assays include biolistic bombardment, protoplast transfection and Agrobacterium-mediated transient transformation, each having advantages and disadvantages depending on the research purposes. Results We present a novel transient assay based on cocultivation of young Arabidopsis (Arabidopsis thaliana seedlings with Agrobacterium tumefaciens in the presence of a surfactant which does not require any dedicated equipment and can be carried out within one week from sowing seeds to protein analysis. This Fast Agro-mediated Seedling Transformation (FAST was used successfully to express a wide variety of constructs driven by different promoters in Arabidopsis seedling cotyledons (but not roots in diverse genetic backgrounds. Localizations of three previously uncharacterized proteins were identified by cotransformation with fluorescent organelle markers. The FAST procedure requires minimal handling of seedlings and was also adaptable for use in 96-well plates. The high transformation efficiency of the FAST procedure enabled protein detection from eight transformed seedlings by immunoblotting. Protein-protein interaction, in this case HY5 homodimerization, was readily detected in FAST-treated seedlings with Förster resonance energy transfer and bimolecular fluorescence complementation techniques. Initial tests demonstrated that the FAST procedure can also be applied to other dicot and monocot species, including tobacco, tomato, rice and switchgrass. Conclusion The FAST system provides a rapid, efficient and economical assay of gene function in intact plants with minimal manual handling and without dedicated device. This method is potentially

  6. Foliar uptake of nitrogen from ant fecal droplets: an overlooked service to ant plants

    DEFF Research Database (Denmark)

    Pinkalski, Christian Alexander Stidsen; Jensen, Karl-Martin Vagn; Damgaard, Christian Frølund

    2017-01-01

    facultative relationships, where ants forage on plants. 2. In a laboratory setupexperiment, weaver ants (Oecophylla smaragdina) were confined to the canopies of coffee (Coffea arabica) seedlings, excluding any ant-to-plant transfer of nutrients via the soil strata. When ants were fed 15N-labelled glycine......-recognised but probably more fluctuating benefit from herbivore protection. Given the worldwide abundance of plant canopies foraged by ants, this nutrient pathway may be of high ecological significance.......1. Nutrient supplies to plants from ants are well known from specialised myrmecophytic symbioses and from plants growing in soil close to ant nests. However, above ground nutrient pathways may play a hitherto largely unrecognised role also in less specialised ant-plant interactions – the numerous...

  7. Transformational leadership, transnational culture and political competence in globalizing health care services: a case study of Jordan's King Hussein Cancer Center

    Directory of Open Access Journals (Sweden)

    Pappas Gregory

    2007-11-01

    Full Text Available Abstract Background Following the demise of Jordan's King Hussein bin Talal to cancer in 1999, the country's Al-Amal Center was transformed from a poorly perceived and ineffectual cancer care institution into a Western-style comprehensive cancer center. Renamed King Hussein Cancer Center (KHCC, it achieved improved levels of quality, expanded cancer care services and achieved Joint Commission International accreditation under new leadership over a three-year period (2002–2005. Methods An exploratory case research method was used to explain the rapid change to international standards. Sources including personal interviews, document review and on-site observations were combined to conduct a robust examination of KHCC's rapid changes. Results The changes which occurred at the KHCC during its formation and leading up to its Joint Commission International (JCI accreditation can be understood within the conceptual frame of the transformational leadership model. Interviewees and other sources for the case study suggest the use of inspirational motivation, idealized influence, individualized consideration and intellectual stimulation, four factors in the transformational leadership model, had significant impact upon the attitudes and motivation of staff within KHCC. Changes in the institution were achieved through increased motivation and positive attitudes toward the use of JCI continuous improvement processes as well as increased professional training. The case study suggests the role of culture and political sensitivity needs re-definition and expansion within the transformational leadership model to adequately explain leadership in the context of globalizing health care services, specifically when governments are involved in the change initiative. Conclusion The KHCC case underscores the utility of the transformational leadership model in an international health care context. To understand leadership in globalizing health care services, KHCC

  8. Ultrasonic measurements for in-service assessment of wrought Inconel 625 cracker tubes of heavy water plants

    Energy Technology Data Exchange (ETDEWEB)

    Kumar, Anish [Metallurgy and Materials Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603 102, Tamil Nadu (India); Rajkumar, K.V. [Metallurgy and Materials Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603 102, Tamil Nadu (India); Jayakumar, T. [Metallurgy and Materials Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603 102, Tamil Nadu (India)]. E-mail: tjk@igcar.ernet.in; Raj, Baldev [Metallurgy and Materials Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603 102, Tamil Nadu (India); Mishra, B. [Heavy Water Board, Mumbai 400 085 (India)

    2006-05-01

    The degradation in mechanical properties of Inconel 625 ammonia cracker tubes occurs during the service for long duration in heavy water plants. The present study brings out the possibility of using Poisson's ratio (derived from measurement of time of flight of ultrasonic waves) in combination with hardness measurements, as an effective non-destructive tool for assessment of in-service degradation of Inconel 625 cracker tubes and qualification of re-solution annealing heat treatment for their rejuvenation. Further, the study also indicates the feasibility of extending the life of some of the tubes beyond the presently followed 120 000 h, before they are taken up for re-solution annealing, without affecting their serviceability. However, further studies are required to identify quantitative criterion for Poisson's ratio and hardness values, for deciding on the basis for removal of the tubes for rejuvenation.

  9. Failure of Titanium Condenser Tubes after 24 Years Power Plant Service

    DEFF Research Database (Denmark)

    Montgomery, Melanie; Enemark, Allan; Hangaard, Anders

    2014-01-01

    a plant trip. In addition, small amounts of titanium hydride were revealed to be present in the tubes within the tubesheet indicating that the carbon steel tubesheet was corroding due to ingress of salt water. Although this was not the reason for the failure, it indicated the need for repair of the epoxy......The titanium condenser has been in operation for 24 years at Amager unit 3 power plant. In February 2012, the plant was contaminated by seawater due to a failed condenser tube and some tubes were plugged. A month later, the plant tripped again. Small leaks were found again and finally approx. 200...

  10. Web-Browsing Competencies of Pre-Service Adult Facilitators: Implications for Curriculum Transformation and Distance Learning

    Science.gov (United States)

    Theresa, Ofoegbu; Ugwu, Agboeze Matthias; Ihebuzoaju, Anyanwu Joy; Uche, Asogwa

    2013-01-01

    The study investigated the Web-browsing competencies of pre-service adult facilitators in the southeast geopolitical zone of Nigeria. Survey design was adopted for the study. The population consists of all pre-service adult facilitators in all the federal universities in the southeast geopolitical zone of Nigeria. Accidental sampling technique was…

  11. "Baby Teachers": How Pre-Service Early Childhood Students Transform Their Conceptions of Teaching and Learning through an Infant Practicum

    Science.gov (United States)

    Recchia, Susan L.; Shin, Minsun

    2010-01-01

    This qualitative study explores how working with infants changed pre-service early childhood students' thinking about important aspects of early childhood education. Through a qualitative analysis of four pre-service early childhood students' weekly dialogue journals during a required, one-semester infant practicum, the authors discovered how the…

  12. Field Feeding Transformation: Is There a Requirement for Food Service Personnel in Support of the Stryker Brigade Combat Team?

    Science.gov (United States)

    2002-01-01

    under the commercial industry food protection standard from the National Restaurant Association called ServSafe . This article confirms the need and...Restaurant Association’s Applied Food Service Sanitation course ( SERVSAFE ). 2. Quartermaster Center and School’s Food Service Sanitation Course to include

  13. Practicum as a Space and Time of Transformation: Self-Narrative of a Physical Education Pre-Service Teacher

    Science.gov (United States)

    Margarida, Alves; Ana, Pereira; Amandio, Graca; Paula, Batista

    2012-01-01

    Understanding the role of the practicum in the PI (professional identity) construction and reconstruction within a pre-service teacher is crucial, since this is a remarkable moment in their initial training. This moment provides new feelings and challenges, and it represents the transition from students (pre-service teacher) to teachers…

  14. Exploring Indigenous Game-Based Physics Activities in Pre-Service Physics Teachers' Conceptual Change and Transformation of Epistemic Beliefs

    Science.gov (United States)

    Morales, Marie Paz Escaño

    2017-01-01

    "Laro-ng-Lahi" (Indigenous Filipino game) based physics activities invigorated the integration of culture in the pre-service physics education to develop students' epistemic beliefs and the notion of conceptual understanding through conceptual change. The study conveniently involved 28 pre-service undergraduate physics students enrolled…

  15. Exploring Indigenous Game-Based Physics Activities in Pre-Service Physics Teachers' Conceptual Change and Transformation of Epistemic Beliefs

    Science.gov (United States)

    Morales, Marie Paz Escaño

    2017-01-01

    "Laro-ng-Lahi" (Indigenous Filipino game) based physics activities invigorated the integration of culture in the pre-service physics education to develop students' epistemic beliefs and the notion of conceptual understanding through conceptual change. The study conveniently involved 28 pre-service undergraduate physics students enrolled…

  16. Transformation of tobacco plant (Nicotiana tabacum L. with the recombinant hepatitis B virus genes 35SHBsAg and 35SHBsAgER

    Directory of Open Access Journals (Sweden)

    Juliana Martins Ribeiro

    2010-03-01

    Full Text Available The recombinant surface antigen of hepatitis B virus (HBsAg, purified from transgenic plants, proved to be efficient when utilized for raising anti-HB antibodies for the prevention of hepatitis B. Because of the important role of the HBsAg antigen in hepatitis B prevention, the coding sequence of HBsAg antigen, with or without the addition of the carboxi-terminus sequence for protein retention in the endoplasmatic reticulum, was linked to cauliflower mosaic virus 35S promoter, tobacco mosaic virus leader sequence Ω, and the transcription terminator sequence. The aim of this work was to clone the chimeric gene 35SHBsAgER in the plant expression vector pGPTV/Kan/Asc. The resulting plasmid, called pG35SHBsAgER, and another plasmid produced previously in our laboratory called pG35SHBsAg, were transferred to Agrobacterium tumefaciens, and tobacco leaves, of the SR1 cultivar were used as explants for genetic transformation. Twenty-one fully regenerated plants were obtained (10 for the pG35SHBsAg construction and 11 for the pG35SHBsAgER construction. The genomic DNA of all plants was analyzed by PCR, and the presence of the transgene was confirmed in all plants.

  17. Environmental risk assessment for plant pests: a procedure to evaluate their impacts on ecosystem services

    DEFF Research Database (Denmark)

    Gilioli, G.; Schrader, G.; Baker, R.H.A.;

    2014-01-01

    . By including both the structural and functional components of the environment threatened by invasive alien species (IAS), in particular plant pests, we propose an environmental risk assessment scheme that addresses this complexity. Structural components are investigated by evaluating the impacts of the plant...

  18. 核电工程变压器过励磁能力研究%Study on Over-excitation Capability of Nuclear Power Plant Transformer

    Institute of Scientific and Technical Information of China (English)

    岳新亮; 贾鸿斌; 李英; 邹宇星

    2015-01-01

    由于出口核电工程用变压器运行环境的特殊性(低频率、电压不稳定),目前国家标准中的过励磁曲线无法满足发电机变压器组保护整定值计算的需要,对发电机、变压器乃至核电厂的安全运行都有极大的隐患.文章进行变压器过励磁能力的计算分析,开展了硅钢片材料在频率变化时的性能测试,对比了过电压、降频率两种方式或两种条件同时变化的过励磁,得出了设计此类变压器和二次整定保护的一些建议.%Due to the particularity of the operation environment (low frequency, voltage instability) of export nuclear power plant transformer, the current national standard of the over-excitation curve cannot meet the requirement of protection setting value calculation, which has hidden risk for the safe operation of generator, transformer and even the whole nuclear power plant. In this regard, calculation and analysis are carried out for the transformer over-excitation ability. Performance tests are carried out for the silicon steel sheet material in frequency changes. The over-excitation is compared for two ways of over-pressure and frequency reduction or two conditions change at the same time. Some suggestions are given for the design of such transformer, and protection of the secondary setting.

  19. THE EXPERIENCE OF THE TRANSFORMATION OF SOME CULTIVATED PLANTS WITH THE GENE UGT ENCODING THE SYNTHESIS OF UDPG-TRANSFERASE IN ORDER TO CHANGE THE HORMONAL STATUS

    Directory of Open Access Journals (Sweden)

    Rekoslavskaya N.I.

    2012-08-01

    Full Text Available The gene ugt/iaglu was isolated from cDNA library obtained from seedlings of Zea mays L. Positive clones prepared by Lambda ZAPII (Stratagene, USA procedure were screened via western blot with antibodies to UDPG-transferase from corn endosperm raised in rabbit serum. The plasmid pBluescript harboring the gene ugt/iaglu was placed into Escherichia coli (E.coli DH5a under T7/T3 promoter. The gene ugt/iaglu was sequenced and the size was determined as much as 1740 bp. The UDPG-transferase or by trivial name Indoleacetic acid (IAA - glucose synthase (IAGlu-synthase binds IAA with glucose from UDPG thereby making the temporary inactivation and storing of this phytohormone which is capable to be released after the demand from cells. Several cultivated plants were used for transfromation with the gene ugt/iaglu from corn: tomato, potato, lettuce, egg-plant, pepper, strawberry, cucumber, squash, aspen, poplar, pine and others. All plants transformed with the gene ugt/iaglu showed fast growth, better flowering and harvest. The insertion and expression of the gene ugt/iaglu was confirmed in transformed tomato, potato and aspen with PCR, RT-PCR, southern and northern blottings. The contents of free IAA and its bound form IAGlu were higher as much as twice in tomato, potato and aspen transformed with the gene ugt/iaglu. The harvest of tomato was 3-4 times higher in transgenic tomato. The amount of potato tubers and their whole masses were 1.5 - 2 times higher in transgenic potato of several varieties in comparison to control.

  20. Aboriginal and Torres Strait Islander Worldviews and Cultural Safety Transforming Sexual Assault Service Provision for Children and Young People

    Directory of Open Access Journals (Sweden)

    Leticia Funston

    2013-08-01

    Full Text Available Child Sexual Assault (CSA in Aboriginal and Torres Strait Islander communities is a complex issue that cannot be understood in isolation from the ongoing impacts of colonial invasion, genocide, assimilation, institutionalised racism and severe socio-economic deprivation. Service responses to CSA are often experienced as racist, culturally, financially and/or geographically inaccessible. A two-day forum, National Yarn Up: Sharing the Wisdoms and Challenges of Young People and Sexual Abuse, was convened by sexual assault services to identify the main practice and policy concerns regarding working with Aboriginal and Torres Strait Islander children and young people (C&YP, families and communities in the context of CSA. The forum also aimed to explore how services can become more accountable and better engaged with the communities they are designed to support. The forum was attended by eighty invited Aboriginal and Torres Strait Islander and non-Aboriginal youth sexual assault managers and workers representing both “victim” and “those who sexually harm others” services. In keeping with Aboriginal Community-Based Research methods forum participants largely directed discussions and contributed to the analysis of key themes and recommendations reported in this article. The need for sexual assault services to prioritise cultural safety by meaningfully integrating Aboriginal and Torres Strait Islander Worldviews emerged as a key recommendation. It was also identified that collaboration between “victims” and “those who sexually harm” services are essential given Aboriginal and Torres Strait Islander C&YP who sexually harm others may have also been victims of sexual assault or physical violence and intergenerational trauma. By working with the whole family and community, a collaborative approach is more likely than the current service model to develop cultural safety and thus increase the accessibility of sexual assault services.

  1. Тhe transformation of the oxide coatings of aluminum by imitation factors of nuclear power plants

    Directory of Open Access Journals (Sweden)

    V.K. Milinchuk

    2016-09-01

    Full Text Available The authors present the results of a study on kinetics of hydrogen generation by heterogeneous compositions with aluminum exposed to γ-irradiation in air, in different aqueous solutions at room temperature and high temperature annealing. It has been found that hydrogen generation kinetics depends on the γ-irradiation dose, temperature and aqueous medium chemistry. Changes in hydrogen generation kinetics are due to transformations of aluminum oxide coatings influenced by factors simulating the NPP conditions. The effect of metal oxide coating transformations should be considered in predicting the corrosion resistance of NPP structural materials.

  2. Seismic analysis of the Nuclear Fuel Service Reprocessing Plant at West Valley, New York: documentation

    Energy Technology Data Exchange (ETDEWEB)

    Murray, R.C.; Nelson, T.A.; Davito, A.M.

    1977-04-26

    This material was generated as part of a seismic case review of the NFS Reprocessing Plant. This study is documented in UCRL-52266. The material is divided into two parts: mathematical model information, and ultimate load calculations and comparisons. (DLC)

  3. A novel resource-service mutualism between bats and pitcher plants.

    Science.gov (United States)

    Grafe, T Ulmar; Schöner, Caroline R; Kerth, Gerald; Junaidi, Anissa; Schöner, Michael G

    2011-06-23

    Mutualistic relationships between vertebrates and plants apart from the pollen and seed-dispersal syndromes are rare. At first view, carnivorous pitcher plants of the genus Nepenthes seem to be highly unlikely candidates for mutualistic interactions with animals, as they form dimorphic terrestrial and aerial pitchers that trap arthropods and small vertebrates. Surprisingly, however, the aerial pitchers of Nepenthes rafflesiana variety elongata are poor insect traps, with low amounts of insect-attractive volatile compounds and low amounts of digestive fluid. Here, we show that N. rafflesiana elongata gains an estimated 33.8 per cent of the total foliar nitrogen from the faeces of Hardwicke's woolly bats (Kerivoula hardwickii hardwickii) that exclusively roost in its aerial pitchers. This is the first case in which the faeces-trapping syndrome has been documented in a pitcher plant that attracts bats and only the second case of a mutualistic association between a carnivorous plant and a mammal to date.

  4. Plants extracts on the service of production eco-friendly poultry products

    OpenAIRE

    Feofilova, J.; Nesteruk, N.

    2013-01-01

    Plants extracts contain many biologically active compounds, mainly polyphenolics, which possess antimicrobial, antioxidant, antiparasitic, antiprotozoal, antifungal, and anti-inflammatory properties. Currently, they are relevant in nutrition for poultry because they are natural, eco-friendly and generally recognized as safe products. Therefore, aromatic plants and their extracts have the potential to become new generation substances for animal nutrition and health. The purpose of this review ...

  5. The integrated web service and genome database for agricultural plants with biotechnology information

    Science.gov (United States)

    Kim, ChangKug; Park, DongSuk; Seol, YoungJoo; Hahn, JangHo

    2011-01-01

    The National Agricultural Biotechnology Information Center (NABIC) constructed an agricultural biology-based infrastructure and developed a Web based relational database for agricultural plants with biotechnology information. The NABIC has concentrated on functional genomics of major agricultural plants, building an integrated biotechnology database for agro-biotech information that focuses on genomics of major agricultural resources. This genome database provides annotated genome information from 1,039,823 records mapped to rice, Arabidopsis, and Chinese cabbage. PMID:21887015

  6. TRANSFORMACIÓN DE PLANTAS MEDIADA POR AGROBACTERIUM: "INGENIERÍA GENÉTICA NATURAL APLICADA" PLANT TRANSFORMATION MEDIATED BY AGROBACTERIUM: "APPLIED NATURAL GENETIC ENGINEERING"

    Directory of Open Access Journals (Sweden)

    Ana Milena Valderrama Fonseca

    2005-06-01

    Full Text Available Agrobacterium tumefaciens tiene la capacidad de transferir ADN entre reinos diferentes. El impacto de este hallazgo ha tenido grandes aplicaciones en diversos campos de la biología vegetal, agricultura y biotecnología. En este artículo se describen los procesos por los cuales Agrobacterium realiza la transferencia de ADN a la planta, puntualizando en 7 eventos fundamentales para la interacción A. tumefaciens-planta y esta dirigido a profesionales de las áreas biológicas que estén interesados en actualizarse en el tema. El conocimiento básico sobre el mecanismo de transferencia del ADN ha permitido el desarrollo de vectores para la introducción de genes foráneos, dentro de los cuales se describen los 2 tipos de vectores utilizados en la actualidad: co-integrados y binarios. Así mismo se detallan algunos factores importantes que median la transformación y algunas de las principales aplicaciones de la transformación de plantas y hongos mediada por Agrobacterium.Agrobacterium tumefaciens has the ability to transfer DNA between different kingdoms. This finding has had a great impact in several fields of plant biology, agriculture and biotechnology. This review describe the mechanisms by which Agrobacterium transfer DNA to the plant, emphasizing 7 fundamental steps in the A. tumefaciens-plant interaction, to provide an opportunity for professionals in the biological sciences to familiarize themselves with the topic. Basic knowledge about the DNA transfer mechanism has allowed the development of various vectors for the introduction of foreign genes, among which two classes that are currently used are described: co-integrative and binary vectors. Furthermore, detail some important factors that mediate transformation and some of the principal applications of the transformation of plants and fungi by means of Agrobacterium.

  7. Capabilities and costs for ancillary services provision by wind power plants. Deliverable D 3.1

    DEFF Research Database (Denmark)

    Faiella, Mariano; Hennig, Tobias; Cutululis, Nicolaos Antonio;

    This report is the deliverable of the third work package of the REserviceS project and describes the technical options and related costs for the provision of ancillary services specifically from wind energy technologies. It is focused on the set of ancillary services defined in the previous work...... package 2, shown in table 1 below. The information from this deliverable will be used as input to the case studies in subsequent work packages, which are expected to provide additional insights to the actual provision of ancillary services in transmission and distribution networks....

  8. When servitization is not transforming the way we do business - analysis of two unsuccessful service offerings from the shipping industry

    DEFF Research Database (Denmark)

    Pagoropoulos, Aris; Kjær, Louise Laumann; McAloone, Tim C.

    2016-01-01

    with the higher initial cost of the offerings, the customer company essentially challenged the necessity and efficiency of the transformation. The second was the manufacturer’s inability to deliver to the customer’s expectations. Even in cases where the customer was able to communicate its own needs......Purpose: Although servitization as a transformation process is recognized as a source of competitive advantage for both manufacturers and their customers, it has also suffered from limited success, dissatisfactory returns and slow adoption rates. The majority of the studies in literature...... are primarily focused on conceptualizing servitization and recognizing drivers and barriers towards successful implementation from the manufacturer point of view. Far less attention has been given to the customer organisation and to negative results, where manufacturer’s servitization attempts have failed...

  9. Promoter deletions are essential for transformation of lettuce by the T-cyt gene: the phenotypes of transgenic plants

    NARCIS (Netherlands)

    Curtis, H.E.C.; Jordi, W.; Davelaar, E.; Power, J.B.; Laat, de A.M.M.; Davey, M.R.

    1999-01-01

    The Agrobacterium T- cyt gene was transferred into lettuce, Latuca sativa‘Saladin’ using a genotype-independent transformation procedure employing a supervirulent Agrobacterium tumefaciens strain carrying the binary vector pMOG23. Kanamycin-resistant shoots were initiated from inoculated explants on

  10. Promoter deletions are essential for transformation of lettuce by the T-cyt gene: the phenotypes of transgenic plants

    NARCIS (Netherlands)

    Curtis, H.E.C.; Jordi, W.; Davelaar, E.; Power, J.B.; Laat, de A.M.M.; Davey, M.R.

    1999-01-01

    The Agrobacterium T- cyt gene was transferred into lettuce, Latuca sativa‘Saladin’ using a genotype-independent transformation procedure employing a supervirulent Agrobacterium tumefaciens strain carrying the binary vector pMOG23. Kanamycin-resistant shoots were initiated from inoculated explants

  11. Promoter deletions are essential for transformation of lettuce by the T-cyt gene: the phenotypes of transgenic plants

    NARCIS (Netherlands)

    Curtis, H.E.C.; Jordi, W.; Davelaar, E.; Power, J.B.; Laat, de A.M.M.; Davey, M.R.

    1999-01-01

    The Agrobacterium T- cyt gene was transferred into lettuce, Latuca sativa‘Saladin’ using a genotype-independent transformation procedure employing a supervirulent Agrobacterium tumefaciens strain carrying the binary vector pMOG23. Kanamycin-resistant shoots were initiated from inoculated explants on

  12. Transformation and analysis of tobacco plant var Petit havana with T-urf13 gene under anther-specific TA29 promoter.

    Science.gov (United States)

    Arun, V; Kuriakose, Boney; Sridhar, Vaniyambadi V; Thomas, George

    2011-09-01

    T-urf13, a well-documented cms-associated gene from maize, has been shown to render methomyl sensitivity to heterologous systems like rice, yeast and bacteria when expressed constitutively. Since these transgenic plants were fertile, it was hypothesized that T-urf13 gene if expressed in anthers may result in male sterility that could be used for hybrid seed production. Hence, this work was aimed at analysing whether T-urf13 gene when expressed in anthers can result in male sterile plants or requires methomyl treatment to cause male sterility (controllable). This is the first report of transformation of tobacco with T-urf13 gene under anther-specific promoter (TA29) with or without mitochondrial targeting sequence. Most of the transgenic plants obtained were fertile; this was surprising as many male sterile plants were expected as T-urf13 gene is a cms associated gene. Our results suggest that it may not be possible to obtain male sterility by expressing URF13 in the anther by itself or by methomyl application.

  13. Accumulation of human EGF in nectar of transformed plants of Nicotiana langsdorffii x N. sanderae and transfer to honey by bees.

    Science.gov (United States)

    Helsper, J P F G; Ruyter-Spira, C P; Kwakman, P H S; Bleeker, W K; Keizer, L C P; Bade, J B; Te Velde, A A; Zaat, S A J; Verbeek, M; Creemers-Molenaar, J

    2011-09-01

    Honey has been used successfully in wound healing for thousands of years. The peptide hormone human epidermal growth factor (hEGF) is also known to have a beneficial effect in various wound healing processes via mechanisms that differ from those for honey. In this study, we show that hEGF can be incorporated into honey via nectar. Plants of Nicotiana langsdorffii x N. sanderae were transformed with the gene for hEGF, equipped with a nectary-targeted promoter and a signal sequence for secretion to nectar. These plants accumulated hEGF in the nectar. The maximum hEGF concentration recorded with ELISA in these plants is 2.5 ng·ml⁻¹. There is a significant linear relationship (Pplants did not allow production of honey from their nectar, we used feeding solutions, spiked with synthetic hEGF, to study transfer of this peptide into honey through bee activity. Transfer of hEGF from a feeding solution to honey by bees occurred with retention of the hEGF concentration and the capacity to induce hEGF-receptor phosphorylation. These observations indicate that plants can function as a production platform for honey containing biologically active peptides, which may enhance wound healing and other biological processes.

  14. Tobacco plants transformed with the bean. alpha. ai gene express an inhibitor of insect. alpha. -amylase in their seeds. [Nicotiana tabacum; Tenebrio molitor

    Energy Technology Data Exchange (ETDEWEB)

    Altabella, T.; Chrispeels, M.J. (Univ. of California, San Diego, La Jolla (USA))

    1990-06-01

    Bean (Phaseolus vulgaris L.) seeds contain a putative plant defense protein that inhibits insect and mammalian but not plant {alpha}-amylases. We recently presented strong circumstantial evidence that this {alpha}-amylase inhibitor ({alpha}Al) is encoded by an already-identified lectin gene whose product is referred to as lectin-like-protein (LLP). We have now made a chimeric gene consisting of the coding sequence of the lectin gene that encodes LLP and the 5{prime} and 3{prime} flanking sequences of the lectin gene that encodes phytohemagglutinin-L. When this chimeric gene was expressed in transgenic tobacco (Nicotiana tabacum), we observed in the seeds a series of polypeptides (M{sub r} 10,000-18,000) that cross-react with antibodies to the bean {alpha}-amylase inhibitor. Most of these polypeptides bind to a pig pancreas {alpha}-amylase affinity column. An extract of the seeds of the transformed tobacco plants inhibits pig pancreas {alpha}-amylase activity as well as the {alpha}-amylase present in the midgut of Tenebrio molitor. We suggest that introduction of this lectin gene (to be called {alpha}ai) into other leguminous plants may be a strategy to protect the seeds from the seed-eating larvae of Coleoptera.

  15. Efficient power plant operation due to communication standards. Application for inspection, service and maintenance; Effizienterer Kraftwerksbetrieb dank offener Kommunikationsstandards. Nutzen fuer Inspektion, Wartung und Instandhaltung

    Energy Technology Data Exchange (ETDEWEB)

    Brandt, Gerhard [ABB AG, Mannheim (Germany). Geschaeftsbereich Stromerzeugung

    2011-07-01

    Advanced device engineering and actual open communication standards in power plants offer comprehensive information, more than traditional engineering can present. By intelligent utilization of so gained different informations power plant process control systems can generate for the operator added value during operation, in special inspection, service and maintenance. (orig./GL)

  16. Risk-constrained dynamic self-scheduling of a pumped-storage plant in the energy and ancillary service markets

    Energy Technology Data Exchange (ETDEWEB)

    Kazempour, S. Jalal; Moghaddam, M. Parsa; Haghifam, M.R.; Yousefi, G.R. [Department of Electrical Engineering, Tarbiat Modares University (TMU), Tehran (Iran)

    2009-05-15

    This work addresses a new framework for self-scheduling of an individual price-taker pumped-storage plant in a day-ahead (DA) market. The goal is achieving the best trade-off between the expected profit and the risks when the plant participates in DA energy, spinning reserve and regulation markets. In this paper, a set of uncertainties including price forecasting errors and also the uncertainty of power delivery requests in the ancillary service markets are contemplated. Considering these uncertainties, a new approach is proposed which is called dynamic self-scheduling (DSS). This risk-constrained dynamic self-scheduling problem is therefore formulated and solved as a mixed integer programming (MIP) problem. Numerical results for a case study are discussed. (author)

  17. A biome-scale assessment of the impact of invasive alien plants on ecosystem services in South Africa.

    Science.gov (United States)

    van Wilgen, B W; Reyers, B; Le Maitre, D C; Richardson, D M; Schonegevel, L

    2008-12-01

    This paper reports an assessment of the current and potential impacts of invasive alien plants on selected ecosystem services in South Africa. We used data on the current and potential future distribution of 56 invasive alien plant species to estimate their impact on four services (surface water runoff, groundwater recharge, livestock production and biodiversity) in five terrestrial biomes. The estimated reductions in surface water runoff as a result of current invasions were >3000 million m(3) (about 7% of the national total), most of which is from the fynbos (shrubland) and grassland biomes; the potential reductions would be more than eight times greater if invasive alien plants were to occupy the full extent of their potential range. Impacts on groundwater recharge would be less severe, potentially amounting to approximately 1.5% of the estimated maximum reductions in surface water runoff. Reductions in grazing capacity as a result of current levels of invasion amounted to just over 1% of the potential number of livestock that could be supported. However, future impacts could increase to 71%. A 'biodiversity intactness index' (the remaining proportion of pre-modern populations) ranged from 89% to 71% for the five biomes. With the exception of the fynbos biome, current invasions have almost no impact on biodiversity intactness. Under future levels of invasion, however, these intactness values decrease to around 30% for the savanna, fynbos and grassland biomes, but to even lower values (13% and 4%) for the two karoo biomes. Thus, while the current impacts of invasive alien plants are relatively low (with the exception of those on surface water runoff), the future impacts could be very high. While the errors in these estimates are likely to be substantial, the predicted impacts are sufficiently large to suggest that there is serious cause for concern.

  18. Establishment of Suspension Cell Culture from Agrobacterium-transformed Hairy Root Cells of Psammosilene tunicoides, an Endangered and Rare Medicinal Plant of China

    Directory of Open Access Journals (Sweden)

    Zhang Zong-Shen

    2015-08-01

    Full Text Available Psammosilene tunicoides is an important medicinal plant endemic in China. Its annual yield is severely limited due to slow growth, poor seed germination and excessive collection. To satisfy the growing market demands, it’s necessary to seek alternatives to field cultivation and wild resources of this endangered plant. Using Agrobacterium -transformed hairy roots as initial explants, here, we reported the development of a suspension cell culture system for P. tunicoides. Results showed the Agrobacterium -transformed hairy roots-derived suspension cells are fast in growth and strong in capacity for accumulation of bioactive metabolites. We established that 1/2MS was a suitable medium for culturing the hairy root-derived suspension cells and the optimal combination of phytohormones is 1.5 mg/L 2, 4-D+0.5 mg/L 6-BA+0.25 mg/L NAA+0.1 mg/L KT. Under this condition, the maximal biomass was achieved at the 20th day of culture with an average growth rate of 0.72 g/L/d; and the intracellular saponine content reached 0.92%, comparable to that of mother hairy roots. Compared with the normal P. tunicoides suspension cells, the hairy roots-derived suspension cells exhibited features of fast growth, short culture period and high concentration of saponines, suggesting that the large scale culture of hairy root-derived cells could be a feasible alternative to the wild resources of P. tunicoides.

  19. Development of an efficient in vitro plant regeneration system amenable to Agrobacterium- mediated transformation of a recalcitrant grain legume blackgram (Vigna mungo L. Hepper).

    Science.gov (United States)

    Sainger, Manish; Chaudhary, Darshna; Dahiya, Savita; Jaiwal, Ranjana; Jaiwal, Pawan K

    2015-10-01

    An efficient, rapid and direct multiple shoot regeneration system amenable to Agrobacterium-mediated transformation from primary leaf with intact petiole of blackgram (Vigna mungo) is established for the first time. The effect of the explant type and its age, type and concentration of cytokinin and auxin either alone or in combination and genotype on multiple shoot regeneration efficiency and frequency was optimized. The primary leaf explants with petiole excised from 4-day-old seedlings directly developed multiple shoots (an average of 10 shoots/ explant) from the cut ends of the petiole in 95 % of the cultures on MSB (MS salts and B5 vitamins) medium containing 1.0 μM 6-benzylaminopurine. Elongated (2-3 cm) shoots were rooted on MSB medium with 2.5 μM indole-butyric acid and resulted plantlets were hardened and established in soil, where they resumed growth and reached maturity with normal seed set. The regenerated plants were morphologically similar to seed-raised plants and required 8 weeks time from initiation of culture to establish them in soil. The regeneration competent cells present at the cut ends of petiole are fully exposed and are, thus, easily accessible to Agrobacterium, making this plant regeneration protocol amenable for the production of transgenic plants. The protocol was further successfully used to develop fertile transgenic plants of blackgram using Agrobacterium tumefaciens strain EHA 105 carrying a binary vector pCAMBIA2301 that contains a neomycin phosphotransferase gene (nptII) and a β-glucuronidase (GUS) gene (uidA) interrupted with an intron. The presence and integration of transgenes in putative T0 plants were confirmed by polymerase chain reaction (PCR) and Southern blot hybridization, respectively. The transgenes were inherited in Mendelian fashion in T1 progeny and a transformation frequency of 1.3 % was obtained. This protocol can be effectively used for transferring new traits in blackgram and other legumes for their

  20. [Plants regeneration from genetically transformed root and callus cultures of periwinkle Vinca minor L. and foxglove purple Digitalis purpurea L].

    Science.gov (United States)

    Leshina, L G; Bulko, O V

    2014-01-01

    Plants regenerated from hairy roots and calluses of foxglove purple and periwinkle have been obtained. It was found that organogenesis in hairy root culture occurs spontaneously on hormone-free medium but with different efficiencies. The frequency of direct shoot formation from root cultures was up to 60% in Digitalis and 3.7% in Vinca. Addition of 1 mg/l BA, 0.1 mg/l NAA and 5% sucrose to B5 medium increased regenerative capacity of Vinca roots up to 19.1%. Regenerated plants showed morphological features typically seen in Ri-transgenic plants. They include growth and plagiotropism of the root system, increased shoot formation, changed leaf morphology and short internodes.

  1. Transforming Teaching and Learning at University of Ghana through Community Service-Learning: Listening to the Voices of Students

    Science.gov (United States)

    Tagoe, Michael A.

    2014-01-01

    Universities all over the world are undergoing change to improve teaching, learning and service. These changes have been motivated by call for universities to connect more to communities to address their problems. One of the means of ensuring that universities and communities engage mutually in a partnership where students, faculty and community…

  2. Community Development Workers Programme: Mentoring for Social Transformation in the Public Service in Post-Apartheid South Africa

    Science.gov (United States)

    Geber, Hilary; Motlhake, Bona

    2008-01-01

    The new public sector community development workers (CDWs) programme was established in 2004 following ineffective service delivery through chronic under-spending on annual budgets in post-apartheid South Africa. CDWs receive training in learnerships within the National Skills Development Strategy to ensure access to and spending of local…

  3. Uncertainty Management and Sensemaking as Precursors to Transformative Learning in an International Immersion Service-Learning Experience

    Science.gov (United States)

    Larson, Jan M.; Fay, Martha

    2016-01-01

    This study is based on an international immersion service-learning/research experience in a remote village in Moldova that provided faculty and students an opportunity to teach journalism and help local students and community representatives create their own online news outlet. Students' existing conceptions were challenged, they experienced…

  4. Transforming School Mental Health Services: Population-Based Approaches to Promoting the Competency and Wellness of Children

    Science.gov (United States)

    Doll, Elizabeth J., Ed.; Cummings, Jack A., Ed.

    2007-01-01

    This book provides a "roadmap" for designing and implementing school psychological services for all students in a school. School psychologists will find guidelines for conducting a mental health needs assessment of the entire student body; analyzing patterns and trends to identify risk factors and common needs; and using the needs assessment to…

  5. Construction and characterization of a plant transformation-competent BIBAC library of the black Sigatoka-resistant banana Musa acuminata cv. Tuu Gia (AA).

    Science.gov (United States)

    Ortiz-Vázquez, E; Kaemmer, D; Zhang, H-B; Muth, J; Rodríguez-Mendiola, M; Arias-Castro, C; James, Andrew

    2005-02-01

    A plant transformation-competent binary bacterial artificial chromosome (BIBAC) library was constructed from Musa acuminata cv. Tuu Gia (AA), a black Sigatoka-resistant diploid banana. After digestion of high-molecular-weight banana DNA by HindIII, several methods of DNA size selection were tested, followed by ligation, using a vector/insert molar ratio of 4:1. The library consists of 30,700 clones stored in 80 384-well microtiter plates. The mean insert size was estimated to be 100 kb, and the frequency of inserts with internal NotI sites was 61%. The majority of insert sizes fell into the range of 100+/-20 kb, making them suitable for Agrobacterium-mediated transformation. Only 1% and 0.9% of the clones contain chloroplast and mitochondrial DNA, respectively. This is the first BIBAC library for banana, estimated to represent five times its haploid genome (600 Mbp). It was demonstrated by hybridization that the library contains typical members of resistance gene and defense gene families that can be used for transformation of disease susceptible banana cultivars for banana genetic improvement.

  6. Sewage Treatment Plant inverted A2/O Process Transformation Practice%污水处理厂倒置A2/O工艺改造实践

    Institute of Scientific and Technical Information of China (English)

    姚雪芳

    2014-01-01

    结合工程实例,针对污水处理厂传统A2/O工艺存在的一些不足,提出了采用倒置A2/O工艺进行改造处理,详细阐述了倒置A2/O工艺的改造方案,并对改造后的效果进行研究。实际运行结果表明,改造后的倒置A2/O工艺满足污水处理的要求,出水水质能稳定达到一级A排放标准,具有极大的环境效益和经济效益。%Effect with engineering examples, the sewage treatment plant for a number of shortcomings of traditional A 2/O process exists proposed by inverted A 2/O process to transform treatment elaborated inverted A 2/O process of rehabilitation programs, and after the transformation: Abstract research. Actual operating results show that after the transformation of the inverted A 2/O process to meet the requirements of wastewater treatment, water quality can achieve an A stable emission standards, has great environmental and economic benefits.

  7. Review of shell-and-tube heat exchanger fouling and corrosion in geothermal power plant service

    Energy Technology Data Exchange (ETDEWEB)

    Ellis, P.F. II

    1983-12-01

    Heat exchangers for hot geofluid/working substance vaporizers for binary power plants are considered. A brief description of the physical test apparatus and the geofluid chemistry for each of the several heat exchanger tests is presented. The fouling data developed from these tests are summarized, in most cases presenting a mathematical expression for the increase in fouling factor with time. The materials performance data developed from these same tests are explored. The performance of shell-and-tube heat exchangers used as condensers and ancillary coolers in the power plant heat rejection system is considered.

  8. Public meeting: Western New York Nuclear Service Center options study. [Problem of West Valley plant

    Energy Technology Data Exchange (ETDEWEB)

    None

    1978-04-01

    This document is a transcript of the meeting, with additional written comments. The main topic is the West Valley Processing Plant and how to dispose of it and its high-level wastes. Objective is to get public input on this topic. (DLC)

  9. Global production networks, offshore services and the branch-plant syndrome

    NARCIS (Netherlands)

    Kleibert, J.M.

    2015-01-01

    This article combines the global production network (GPN) framework with the branch-plant economy literature to generate a set of indicators for a more nuanced understanding of the outcomes of foreign direct investment attraction for recipient regions. It adapts both literatures to the case of offsh

  10. Global production networks, offshore services and the branch-plant syndrome

    NARCIS (Netherlands)

    Kleibert, J.M.

    2016-01-01

    This article combines the global production network (GPN) framework with the branch-plant economy literature to generate a set of indicators for a more nuanced understanding of the outcomes of foreign direct investment attraction for recipient regions. It adapts both literatures to the case of offsh

  11. Rice transformation with cell wall degrading enzyme genes from Trichoderma atroviride and its effect on plant growth and resistance to fungal pathogens

    Institute of Scientific and Technical Information of China (English)

    Liu Mei; Sun Zong-Xiu; Zhu Jie; Xu Tong; Gary E Harman; Matteo Lorito; Sheri Woo

    2004-01-01

    @@ Three genes encoding for fungal cell wall degrading enzymes (CWDE), ech42, nag70 and gluc78from the biocontrol fungus Trichoderma atroviride were inserted into the binary vector pCAMBIA1305. 2 singly and in all possible combinations. The coding sequences were placed downstream of the rice actin promoter and all vectors were used to transform rice plants. A total of more than 1,800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. Expression in plant was obtained for all the fungal genes used singly or in combinations. The ech42 gene encoding for an endochitinase increased resistance to sheath blight caused by Rhizoctonia solani, while the exochitinase-encoding gene, nag70, had a lesser effect. The expression level of endochitinase but not of the exochitinase was correlated with disease resistance. Nevertheless, exochitinase enhanced the positive effect of endochitinase on disease resistance when two genes were co-expressed in transgenic rice. Improved resistance to Magnaporthe grisea was found in all types of regenerated plants, including those with the gluc78 gene alone, while a few lines expressing either ech42 or nag70 appeared to be immune to this pathogen. Transgenic plants expressing the gluc78 gene alone were stunted and only few of them survived, even though they showed resistance to M. grisea. However, combination with either one of the two other genes ( ech42, nag70 ) as included in the same T-DNA region, reduced the negative effect of gluc78 on plant growth. This is the first report of single or multiple of expression of transgens encoding CWDEs that results in resistance to blast and sheath blight in rice.

  12. Steam oxidation of TP 347H FG. Laboratory exposures versus service conditions at the power plant

    Energy Technology Data Exchange (ETDEWEB)

    Hansson, Anette N. [DONG Energy A/S, Copenhagen (Denmark); Montgomery, Melanie [DONG Energy A/S, Copenhagen (Denmark); Technical Univ. of Denmark, Lyngby (Denmark). Dept. of Mechanical Engineering; Vattenfall Heat Nordic, Copenhagen (Denmark)

    2010-07-01

    TP347H FG is often used as final superheater tubing at Danish Power Plants. The oxidation behaviour of TP347H FG in steam was investigated both in laboratory conditions and field conditions. Short time exposures (336 hours) were performed in the laboratory at 500, 600 and 700 C in gasses with 8 or 46% H{sub 2}O and varying oxygen partial pressures. The shortest exposure time at the power plant was 7720 h, the temperature varied between 500 and 650 C. Surprisingly, thicker oxide layers formed within the laboratory facility at 600 and 700 C than during the long time exposures at the power plant. This could not be explained by spallation. Double-layered oxides developed during oxidation. The outer layer consist of Fe-oxides and the inner oxide contained Fe and the remaining alloy elements. Investigations with scanning electron microscopy (SEM) revealed that the morphology of the inner oxide was different for the two types of exposures. However, investigation using transmission electron microscopy (TEM) showed that the inner oxide in both cases consisted of particles of Fe-Mn-Cr spinel embedded in a metallic Fe-Ni matrix in the bulk of the (former) alloy grains and Cr-rich oxide layer along the (former) alloy grain boundaries. The main difference between the layers formed at the two locations is that the Cr-rich oxide layer is thicker for the samples exposed at the power plant than that for the samples exposed at the laboratory conditions. Furthermore, the depth of Cr depletion in the alloy adjacent the oxide layer is greater for the samples exposed at the power plant compared to those exposed in the laboratory. The microstructure investigation suggests that the slower oxidation rate of TP347H FG at the power plant as compared to the laboratory is due to a larger reservoir of Cr for the samples exposed at the power plant probably combined with a higher mobility of Cr within the alloy. (orig.)

  13. Medical services of Croat people in Bosnia and Herzegovina during 1992-1995 war: losses, adaptation, organization, and transformation.

    Science.gov (United States)

    Bagaric, I

    2000-06-01

    During the 1992-1995 war in Bosnia and Herzegovina (BH), Croatian people in BH had 19,600 (2.6%) killed and 135,000 (17.6%) displaced persons, and 222,500 (28.9%) refugees. They lost around two thirds of both physicians and other health personnel, and were left with 8. 5% of prewar patient beds. Fortunately, the organized defence against Serbs was initiated in time and Croats defended the territories where they formed majority. The first defense unit established was the Medical Corps Headquarters (MCH), caring for soldiers and civilians alike. The MCH was soon incorporated in the Croatian Defense Council (CDC, armed forces of Croatian people in BH). The MCH had two chains of command. One went through the district commanders of medical services and their subordinated physicians to paramedics in military units, and the other directly to the commanders of 14 war hospitals. After its formation in 1993, the Ministry of Health took the jurisdiction over the civilian medical services and after the Washington Peace Agreement (April 1994) over the war hospitals, too, whereas the medical services within military units remained under control of the Ministry of Defense. Dayton Peace Agreement divided BH into the Federation of BH and Republic Srpska, each with their own army. The Federation of BH Army is composed of the CDC and Bosniac-controlled Army of BH, with overall numerical ratio 1:2.3 for Bosniacs, and organized in accordance with NATO standards. Military medical services are provided by the Logistics Sector of both Ministry of Defense and Military Corps Headquarters (Joint Command).

  14. Technical Feasibility of Ancillary Services provided by ReGen Plants

    DEFF Research Database (Denmark)

    Altin, Müfit; Han, Xue; Hansen, Anca Daniela;

    This report is the first deliverable in WP1 in the project “Ancillary services from renewable power plants” (RePlan). RePlan is funded as PSO project 2015 no. 12347 by the Danish PSO-programme ForskEL, which is administered by Energinet.DK. RePlan is carried out in collaboration between DTU Wind ...

  15. From the Field: Windows of Opportunity – The Transformation of State Media to Public Service Media in Kyrgyzstan, Mongolia, Moldova and Serbia

    Directory of Open Access Journals (Sweden)

    Jan Lublinski

    2015-01-01

    Full Text Available The transformation of state media to public service media (PSM is one of the most ambitious endeavors in the field of media development. Not many efforts to free the national media from government control have succeeded in the past decades. In this paper the comparatively promising cases of Kyrgyzstan, Mongolia, Moldova and Serbia are discussed. The PSM in these countries all have a new legal basis, including a public service remit and a relatively independent governing body in which civil society is represented. The services delivered to the public by these media are analyzed according to a number of societal functions which are assembled under two general headings: “creating a public sphere” and “supporting integration”. Based on this analysis, a differentiation between “PSM in initial transformation” and “PSM in advanced transformation” is suggested. In all cases studied, different actors successfully used windows of opportunity: general political agendas to reform the media, a specific engagement from the management as well as support from the population and civil society. Media development actors here helped to advance the processes of change in different ways. Recommendations for future media development include strategic planning, inclusion of local actors, the pooling of legal expertise as well as structured processes of organizational development and capacity building.

  16. Fenton process-affected transformation of roxarsone in paddy rice soils: Effects on plant growth and arsenic accumulation in rice grain.

    Science.gov (United States)

    Qin, Junhao; Li, Huashou; Lin, Chuxia

    2016-08-01

    Batch and greenhouse experiments were conducted to examine the effects of Fenton process on transformation of roxarsone in soils and its resulting impacts on the growth of and As uptake by a rice plant cultivar. The results show that addition of Fenton reagent markedly accelerated the degradation of roxarsone and produced arsenite, which was otherwise absent in the soil without added Fenton reagent. Methylation of arsenate was also enhanced by Fenton process in the earlier part of the experiment due to abundant supply of arsenate from Roxarsone degradation. Overall, addition of Fenton reagent resulted in the predominant presence of arsenate in the soils. Fenton process significantly improved the growth of rice in the maturity stage of the first crop, The concentration of methylated As species in the rice plant tissues among the different growth stages was highly variable. Addition of Fenton reagent into the soils led to reduced uptake of soil-borne As by the rice plants and this had a significant effect on reducing the accumulation of As in rice grains. The findings have implications for understanding As biogeochemistry in paddy rice field receiving rainwater-borne H2O2 and for development of mitigation strategies to reduce accumulation of As in rice grains.

  17. Plant-wide modelling of phosphorus transformations in wastewater treatment systems: Impacts of control and operational strategies

    DEFF Research Database (Denmark)

    Solon, Kimberly; Flores Alsina, Xavier; Kazadi Mbamba, C

    2017-01-01

    of an ammonium (SNHX ) and total suspended solids (XTSS) control strategy (A1) better adapts the system to influent dynamics, improves phosphate (SPO4) accumulation by phosphorus accumulating organisms (XPAO) (41%), increases nitrification/denitrification efficiency (18%) and reduces aeration energy (Eaeration......, precipitation, mass transfer) process models and model interfaces (between water and sludge line) were required to describe the required tri-phasic (gas, liquid, solid) compound transformations and the close interlinks between the P and the sulfur (S) and iron (Fe) cycles. A modified version of the Benchmark...

  18. Large scale helium liquefaction and considerations for site services for a plant located in Algeria

    Energy Technology Data Exchange (ETDEWEB)

    Froehlich, P.; Clausen, J.J. [Linde Kryotechnik AG, Pfungen (Switzerland)

    2007-07-01

    The large-scale liquefaction of helium extracted from natural gas is depicted. Based on a block diagram the process chain, starting with the pipeline downstream of the natural-gas plant to the final storage of liquid helium, is explained. Information will be provided about the recent experiences during installation and start-up of a bulk helium liquefaction plant located in Skikda, Algeria, including part-load operation based on a reduced feed gas supply. The local working and ambient conditions are described, including challenging logistic problems like shipping and receiving of parts, qualified and semi-qualified subcontractors, basic provisions and tools on site, and precautions to sea water and ambient conditions. Finally, the differences in commissioning (technically and evaluation of time and work packages) to European locations and standards will be discussed. (orig.)

  19. Le composte de qualité au service de la santé des plantes

    OpenAIRE

    Fuchs, Jacques

    2003-01-01

    Les compostes produits selon les règles de l'art ont des propriétés bénéfiques souvent insoupconnées pour les plantes et les sols. Contrairement à la majorité des amendements ultilisés, le compost vit. Cette réalité complique certainement son traitement et son ultilistaion, car, comme tout etre vivant, on doit le traiter avec soin si l'on veut qu'il soit en bonne santé, c'est-à-dire si l'on veut qu'il soit de bonne qualité et ainsi qu'il influence positivement les plantes et les sols. De nomb...

  20. Combining multivariate analysis and monosaccharide composition modeling to identify plant cell wall variations by Fourier Transform Near Infrared spectroscopy

    Directory of Open Access Journals (Sweden)

    Smith-Moritz Andreia M

    2011-08-01

    Full Text Available Abstract We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a mutant population by taking advantage of the Mahalanobis distance outlier scheme to construct a monosaccharide range predictive model using PLS regression. This model was then used to identify specific monosaccharide outliers from the mutant population.

  1. Research on Service Strategy of Supporting Enterprise Service Transformation in Equipment Manufacturing%装备制造配套型企业服务转型的服务策略研究

    Institute of Scientific and Technical Information of China (English)

    王长帅; 余开朝

    2015-01-01

    在制造业服务化的总体趋势下,处于装备制造产业链中低层、数量众多的配套型企业如何定位、改善和开拓其服务,是制造业服务化需要研究的重要课题,其中涉及服务价值评估和服务策略等关键问题。但是,目前国内外关于服务价值评估和服务策略的研究大多是侧重于整机企业与终端客户(B2C或C2B)的。本论文将研究配套厂与整机厂(B2B)的服务策略方法,运用价值分析理论和网络分析法建立服务价值模型,为配套厂的服务策略定位和服务创新提供一种科学思路。%Studying the supporting enterprises, which are the middle-low class in the industry chain of equipment manufacturing, is an important research topic. It is interesting to analyze, in the process of manufacturing servitization, how these large amount of enterprises orient, improve and exploit their services. Specifically, the value assessment of service and service strategy are of the interest. However, we notice that, in the literature, researches of the value and strategy of service mainly concern the whole-machine enterprises and terminal customers (B2C or C2B). In this paper, we will study the service strategy of the auxiliary plant and the whole plant (B2B),and establish the service value model using the value analysis theory and the network analysis method.Finally, to provide a reasonable service suggestion to supporting enterprises.

  2. Discuss on the plant transformation during the implementation of new GMP%新版GMP实施过程中厂房改造的探讨

    Institute of Scientific and Technical Information of China (English)

    王燕

    2016-01-01

    Several aspects, including whether or not the plant should be reconstructed or retransformed during the implementation of new GMP, guarantee of organization system of the implementation of the project, plant design and process layout, the objective and scientiifc risk assessment to the project design, quality control in the progress of project implementation, full estimation of project resources so as to better manage the progress of the project and so on, were described in order to ensure that the company can complete the construction of the plant or the acceptance of its transformation in accordance with the requirements of the project in a limited period of time and to guarantee that the plant can meet the requirements of the new GMP and its intended use and furthermore to provide a reference for the sustained and stable production in the future.%本文对新版GMP的实施过程中是否新建或改造厂房、项目实施的组织体系保证、厂房设计及工艺布局、项目设计环节客观科学的风险评估、项目实施过程中的质量控制、充分预估项目的资源以做好项目的进度管理等方面进行了阐述,以便在有限的时间内既按照工程要求完成厂房的新建或改造验收,又确保厂房符合预定用途及新版GMP的要求,为保证日后持续稳定地生产提供借鉴。

  3. Transformation of tobacco and Arabidopsis plants with Stellaria media genes encoding novel hevein-like peptides increases their resistance to fungal pathogens.

    Science.gov (United States)

    R Shukurov, Rahim; D Voblikova, Vera; Nikonorova, Alexandra K; Komakhin, Roman A; V Komakhina, Vera; A Egorov, Tsezi; V Grishin, Eugene; V Babakov, Alexey

    2012-04-01

    Two novel antifungal hevein-like peptides, SmAMP1.1a and SmAMP2.2a, were previously isolated from seeds of Stellaria media. It has been established that these peptides accumulate in this weed as a result of proteolysis of two propeptides, pro-SmAMP1 and pro-SmAMP2. The primary structure of these propeptides is unique; in addition to having a signal peptide and negatively charged C-terminus, each of these structures consists of two hevein-like peptides of different length separated by a space rather than a single peptide. In this work, we demonstrated that the expression of the pro-SmAMP1 and pro-SmAMP2 genes was tissue-specific and increased substantially under exposure to fungal infection. To elucidate whether S. media has any advantages in defending against phytopathogens due to its unusual structure of pro-SmAMP1 and pro-SmAMP2, on the basis of the pro-SmAMP1 gene, we created three genetic constructs. Arabidopsis and tobacco plants were subsequently transformed with these constructs. Transgenic plants bearing the full-length pro-SmAMP1 gene exhibited the best resistance to the phytopathogens Bipolaris sorokiniana and Thielaviopsis basicola. The resistance of S. media plants to phytopathogenic fungi was likely due to the fungal-inducible expression of pro-SmAMP1 and pro-SmAMP2 genes, and due to the specific features of the primary structure of the corresponding propeptides. As a result of the processing of these propeptides, two different antimicrobial peptides were released simultaneously. Based on our results, we conclude that the genes for antimicrobial peptides from S. media may be promising genetic tools for the improvement of plant resistance to fungal diseases.

  4. Studies of transformational leadership in consumer service: market orientation behavior and alternative roles for the mediators and moderators of change commitment.

    Science.gov (United States)

    Yang, Yi-Feng

    2013-12-01

    The present paper evaluates the relation between transformational leadership and market orientation along with the mediating and moderating effects of change commitment for employees in customer centers in Taiwan. 327 questionnaires were returned by personnel at several customer centers in four different insurance companies. Inter-rater agreement was acceptable based on the multiple raters (i.e., the consumer-related employees from the division groups) of one individual (i.e., a manager)--indicating the aggregated measures were acceptable. The multi-source sample comprised data taken from the four division centers: phone services, customer representatives, financial specialists, and front-line salespeople. The relations were assessed using a multiple mediation procedure incorporating bootstrap techniques and PRODCLIN2 with structural equation modeling analysis. The results reflect a mediating role for change commitment.

  5. To be truly alive: motivation among prison inmate hospice volunteers and the transformative process of end-of-life peer care service.

    Science.gov (United States)

    Cloyes, Kristin G; Rosenkranz, Susan J; Wold, Dawn; Berry, Patricia H; Supiano, Katherine P

    2014-11-01

    Some US prisons are meeting the growing need for end-of-life care through inmate volunteer programs, yet knowledge of the motivations of inmate caregivers is underdeveloped. This study explored the motivations of inmate hospice volunteers from across Louisiana State (n = 75) through an open-ended survey, a grounded theory approach to analysis, and comparison of responses by experience level and gender. Participants expressed complex motivations; Inter-related themes on personal growth, social responsibility and ethical service to vulnerable peers suggested that inmate caregivers experience an underlying process of personal and social transformation, from hospice as a source of positive self-identity to peer-caregiving as a foundation for community. Better understanding of inmate caregiver motivations and processes will help prisons devise effective and sustainable end of life peer-care programs. © The Author(s) 2013.

  6. Nitrogen transformation intensity in wetland plant rhizosphere area at low temperature%低温域湿地植物根际氮转化强度

    Institute of Scientific and Technical Information of China (English)

    黄娟; 李稹; 张健; 朱砺之; 崔明勋; 李卓宪

    2011-01-01

    在低温条件下(0 ~15℃),对菖蒲、芦苇、香蒲等10种湿地植物根际土壤的氮转化强度进行对比分析.结果表明,湿地植物根际氨化、反硝化作用强度随温度下降而逐渐降低,硝化作用强度在10℃左右出现大幅下降;低温域植物根际反硝化作用强度明显高于硝化作用强度;植物种类对根际土壤氨化、硝化作用强度影响较大,其中香蒲氨化作用强度最大(0.85 mg/( kg·h))、慈姑硝化作用强度最大(0.21 mg/(kg·h));植物种类对反硝化作用强度及pH值无明显影响.通过综合评价,低温域香蒲、千屈菜、芦苇根际氮转化强度较强,可作为提高湿地冬季脱氮率的优选耐寒植物.%Nitrogen transformation intensity in rhizosphere soil of ten different wetland plants, such as acorus calamus, phragmites australis and typha orientalis, at low temperatures(0 to 15 ℃) are compared in this paper. The results indicated that: intensity of ammonification and denitrification decrease with decreasing temperature; nitrification intensity decrease sharply while the temperature is under 10 ℃; denitrification intensity is obviously higher than nitrification intensity in plants rhizosphere; the difference of plant species has great impact on ammonification and nitrification intensity; typha orientalis has the strongest ammonification which is 0. 85 mg/(kg ·h) , while sagittaria sagittifolia has the strongest nitrification which is 0. 21 mg/( kg · H) ; plant species has no obvious effect on denitrification and pH in rhizosphere soil. After the comprehensive assessment, typha orientalis, lythrum salicaria and phragmites australis are selected out for nitrogen removal in wetlands at low temperature because of their stronger nitrogen transformation strength in rhizosphere soil.

  7. PAREX, a numerical code in the service of La Hague plant operations

    Energy Technology Data Exchange (ETDEWEB)

    Bisson, J.; Huron, P.; Huel, C. [AREVA NC, La Hague Plant, Technical Direction, 50444, Beaumont-Hague (France); Dinh, B. [CEA, Centre de Marcoule, Nuclear Energy Division, Radiochemistry and Process Department, F-30207, Bagnols-sur-Ceze (France)

    2016-07-01

    The PAREX code developed by the CEA is able to simulate the PUREX process in steady or transient state. From an operator point of view, this numerical code for simulation of liquid-liquid extraction operations is an outstanding tool as an aid for plant operation through process flow sheet optimization, troubleshooting and safety analysis calculations. This paper focuses on two examples. The first concerns the evaluation of the available operating margin of the extraction zone of the first purification cycle flowsheet. The second example concerns a uranium-plutonium splitting operation where the code was used to explain a shift of plutonium concentration in the solvent outlet. (authors)

  8. [The transformation of the dental services market and the battle over a monopoly in 19th century dental practice].

    Science.gov (United States)

    Carvalho, Cristiana Leite

    2006-01-01

    The article analyzes the process by which dentistry acquired the status of a profession. The setting is the mid-nineteenth-century United States, where the West's first professional dental organizations were founded, and the focus is on some aspects of the development of a dental market and on the professional disputes among practitioners of the dental trade, who wanted a monopoly within this field of knowledge. Certain outside factors played a major role in the emergence of the profession, including changes in patterns of sugar consumption (which spread dental caries disease throughout society) as well as the expansion of the dental service market. The subsequent proliferation of distinct groups of dental practitioners--both qualified and unqualified to practice dentistry--and their competition for a place in the dental market reflect the battle waged to establish jurisdiction in this field and the emergence of dentistry as a 'modern profession'.

  9. Service Power Rate and Electricity-Saving in Conventional Thermal Power Plants

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ Since reforming and opening to the outside world, the power construction in China has been expanding by leaps and bounds. As of the end of 2005, the nationwide generation installed capacity made a breakthrough of 500 GW, of which thermal power units represented three-fourths.Relevant predictions indicate that the installed capacity will reach 680-730 GW in 2010 and it will exceed 1000 GW in 2020 as expected. The present situation of service power rate of thermal power units and how to make it approach and come up to world level are questions to be discussed in this paper.

  10. Alloys for 1000 degree C service in the Next Generation Nuclear Plant NERI 05-0191

    Energy Technology Data Exchange (ETDEWEB)

    Gary S. Was; J.W. Jones; T. Pollock

    2009-01-15

    The objective of the proposed research is to define strategies for the improvement of alloys for structural components, such as the intermediate heat exchanger and primary-to-secondary piping, for service at 1000 degree C in the He environment of the NGNP. Specifically, we will investigate the oxidation/carburization behavior and microstructure stability and how these processes affect creep. While generating this data, the project will also develop a fundamental understanding of how impurities in the He environment affect these degradation processes and how this understanding can be used to develop more useful life prediction methodologies.

  11. Characterization of salt tolerance in ectoine-transformed tobacco plants (Nicotiana tabaccum): photosynthesis, osmotic adjustment, and nitrogen partitioning.

    Science.gov (United States)

    Moghaieb, R E A; Tanaka, N; Saneoka, H; Murooka, Y; Ono, H; Morikawa, H; Nakamura, A; Nguyen, N T; Suwa, R; Fujita, K

    2006-02-01

    Ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) biosynthetic genes (ect. ABC) from Halomonas elongata were introduced to tobacco plants using an Agrobacterium-mediated gene delivery system. The genes for ectoine biosynthesis were integrated in a stable manner into the tobacco genome and the corresponding transcripts were expressed. The concentration of ectoine under salt-stress conditions was higher in the roots than in leaves. A close relationship was found between stomatal conductance and the amount of transported nitrogen, suggesting that water transport through the xylem in the stem and transpiration may be involved in nitrogen transport to leaves. The data indicate that the turgor values of the ectoine transgenic lines increased with increasing salt concentration. The data revealed two ways in which ectoine enhanced salinity tolerance of tobacco plants. First, ectoine improved the maintenance of root function so that water is taken up consistently and supplied to shoots under saline conditions. Second, ectoine enhanced the nitrogen supply to leaves by increasing transpiration and by protecting Rubisco proteins from deleterious effects of salt, thereby improving the rate of photosynthesis.

  12. Sewage plant upgrading transformation engineering with MBBR technology%MBBR 工艺用于污水处理厂提标改造工程

    Institute of Scientific and Technical Information of China (English)

    朱智

    2016-01-01

    In light of Yangquan sewage plant upgrading transformation engineering conditions,the paper puts forward MBBR technology as major technology,introduces the engineering technology procedures and design parameters,summarizes its technological demands and properties from aspects of sewage,slurry treatment system and deodorizing system,and finally points out that:the transformation engineering construction achieves great environmental social and economic benefits.%针对阳泉市污水处理厂提标改造工程情况,提出了移动床生物膜( MBBR)工艺作为主体工艺,介绍了该工程的工艺流程及设计参数,并从污水、污泥处理系统、除臭系统方面,对其工艺技术要求及特点进行了总结,指出该工程的改造建设取得了良好的环境、社会及经济效益。

  13. Monitoring of cobalt(II) uptake and transformation in cells of the plant-associated soil bacterium Azospirillum brasilense using emission Mössbauer spectroscopy.

    Science.gov (United States)

    Kamnev, Alexander A; Antonyuk, Lyudmila P; Kulikov, Leonid A; Perfiliev, Yury D

    2004-08-01

    Interaction of cobalt(II) at micromolar concentrations with live cells of the plant-growth-promoting rhizobacterium Azospirillum brasilense (strain Sp245) and further transformations of the metal cation were monitored using 57Co emission Mössbauer spectroscopy (EMS). Cell suspensions of the bacterial culture (2.4 x 10(8) cells ml(-1)) were doped with radioactive 57CoCl2 (1 mCi; final concentration 2 x 10(-6) M 57Co2+), kept under physiological conditions for various periods of time (from 2 min up to 1 hour) and then rapidly frozen in liquid nitrogen. Analysis of emission Mössbauer spectra of the frozen aqueous suspensions of the bacterial cell samples shows that the primary absorption of cobalt(II) at micromolar concentrations by the bacterial cells is rapid and virtually complete, giving at least two major forms of cobalt(II) species bound to the cells. Within an hour, the metal is involved in further metabolic transformations reflected by changes occurring in the spectra. The Mössbauer parameters calculated from the EMS data by statistical treatment were different for suspensions of live and dead (thermally killed) bacterial cells that had been in contact with 57Co2+ for 1 h, as well as for the cell-free culture medium containing the same concentration of 57Co2+. Chemical after-effects of the nuclear transition (57Co --> 5 7Fe), which provide additional information on the chemical environment of metal ions, are also considered. The data presented demonstrate that EMS is a valuable tool for monitoring the chemical state of cobalt species in biological matter providing information at the atomic level in the course of its uptake and/or metabolic transformations.

  14. Beliefs concerning the reliability of nuclear power plant in-service inspections; Uskomuksia ydinvoimalaitoksissa suoritettavien tarkastusten luotettavuudesta

    Energy Technology Data Exchange (ETDEWEB)

    Kettunen, J. [VTT Automation, Espoo (Finland)

    1997-01-01

    The aim of the study was to investigate belief systems held by the officials responsible for the planning and supervision of NDT operations within the Finnish nuclear industry. They were asked to express their opinions on (1) the reliability of NDT methods in general, (2) the factors influencing the reliability of in-service inspections, and (3) the degree of reliability of the current inspections operations conducted by means of NDT methods in the Finnish nuclear power plants. Another goal of the study was to assess the adequacy of officials` beliefs (or belief systems). The research data was collected by interviewing representatives from Finnish power companies (Imatran Voima Oy and Teollisuuden Voima Oy), independent inspection organisations, and the Finnish Centre for Radiation and Nuclear Safety (STUK). The adequacy of the beliefs expressed was assessed by means of the results obtained from international NDT reliability studies and on the basis of interviewees` own justification. (refs.).

  15. Switchgrass (Panicum virgatum L. polyubiquitin gene (PvUbi1 and PvUbi2 promoters for use in plant transformation

    Directory of Open Access Journals (Sweden)

    LaFayette Peter R

    2011-07-01

    Full Text Available Abstract Background The ubiquitin protein is present in all eukaryotic cells and promoters from ubiquitin genes are good candidates to regulate the constitutive expression of transgenes in plants. Therefore, two switchgrass (Panicum virgatum L. ubiquitin genes (PvUbi1 and PvUbi2 were cloned and characterized. Reporter constructs were produced containing the isolated 5' upstream regulatory regions of the coding sequences (i.e. PvUbi1 and PvUbi2 promoters fused to the uidA coding region (GUS and tested for transient and stable expression in a variety of plant species and tissues. Results PvUbi1 consists of 607 bp containing cis-acting regulatory elements, a 5' untranslated region (UTR containing a 93 bp non-coding exon and a 1291 bp intron, and a 918 bp open reading frame (ORF that encodes four tandem, head -to-tail ubiquitin monomer repeats followed by a 191 bp 3' UTR. PvUbi2 consists of 692 bp containing cis-acting regulatory elements, a 5' UTR containing a 97 bp non-coding exon and a 1072 bp intron, a 1146 bp ORF that encodes five tandem ubiquitin monomer repeats and a 183 bp 3' UTR. PvUbi1 and PvUbi2 were expressed in all examined switchgrass tissues as measured by qRT-PCR. Using biolistic bombardment, PvUbi1 and PvUbi2 promoters showed strong expression in switchgrass and rice callus, equaling or surpassing the expression levels of the CaMV 35S, 2x35S, ZmUbi1, and OsAct1 promoters. GUS staining following stable transformation in rice demonstrated that the PvUbi1 and PvUbi2 promoters drove expression in all examined tissues. When stably transformed into tobacco (Nicotiana tabacum, the PvUbi2+3 and PvUbi2+9 promoter fusion variants showed expression in vascular and reproductive tissues. Conclusions The PvUbi1 and PvUbi2 promoters drive expression in switchgrass, rice and tobacco and are strong constitutive promoter candidates that will be useful in genetic transformation of monocots and dicots.

  16. Efficiency of two nitrification inhibitors (dicyandiamide and 3, 4-dimethypyrazole phosphate) on soil nitrogen transformations and plant productivity: a meta-analysis.

    Science.gov (United States)

    Yang, Ming; Fang, Yunting; Sun, Di; Shi, Yuanliang

    2016-02-23

    Dicyandiamide (DCD) and 3, 4-dimethypyrazole phosphate (DMPP) are often claimed to be efficient in regulating soil N transformations and influencing plant productivity, but the difference of their performances across field sites is less clear. Here we applied a meta-analysis approach to compare effectiveness of DCD and DMPP across field trials. Our results showed that DCD and DMPP were equally effective in altering soil inorganic N content, dissolve inorganic N (DIN) leaching and nitrous oxide (N2O) emissions. DCD was more effective than DMPP on increasing plant productivity. An increase of crop yield by DMPP was generally only observed in alkaline soil. The cost and benefit analysis (CBA) showed that applying fertilizer N with DCD produced additional revenues of $109.49 ha(-1) yr(-1) for maize farms, equivalent to 6.02% increase in grain revenues. In comparisons, DMPP application produced less monetary benefit of $15.67 ha(-1) yr(-1). Our findings showed that DCD had an advantage of bringing more net monetary benefit over DMPP. But this may be weakened by the higher toxicity of DCD than DMPP especially after continuous DCD application. Alternatively, an option related to net monetary benefit may be achieved through applying DMPP in alkaline soil and reducing the cost of purchasing DMPP products.

  17. Efficiency of two nitrification inhibitors (dicyandiamide and 3, 4-dimethypyrazole phosphate) on soil nitrogen transformations and plant productivity: a meta-analysis

    Science.gov (United States)

    Yang, Ming; Fang, Yunting; Sun, Di; Shi, Yuanliang

    2016-02-01

    Dicyandiamide (DCD) and 3, 4-dimethypyrazole phosphate (DMPP) are often claimed to be efficient in regulating soil N transformations and influencing plant productivity, but the difference of their performances across field sites is less clear. Here we applied a meta-analysis approach to compare effectiveness of DCD and DMPP across field trials. Our results showed that DCD and DMPP were equally effective in altering soil inorganic N content, dissolve inorganic N (DIN) leaching and nitrous oxide (N2O) emissions. DCD was more effective than DMPP on increasing plant productivity. An increase of crop yield by DMPP was generally only observed in alkaline soil. The cost and benefit analysis (CBA) showed that applying fertilizer N with DCD produced additional revenues of $109.49 ha-1 yr-1 for maize farms, equivalent to 6.02% increase in grain revenues. In comparisons, DMPP application produced less monetary benefit of $15.67 ha-1 yr-1. Our findings showed that DCD had an advantage of bringing more net monetary benefit over DMPP. But this may be weakened by the higher toxicity of DCD than DMPP especially after continuous DCD application. Alternatively, an option related to net monetary benefit may be achieved through applying DMPP in alkaline soil and reducing the cost of purchasing DMPP products.

  18. Plant lectin can target receptors containing sialic acid, exemplified by podoplanin, to inhibit transformed cell growth and migration.

    Directory of Open Access Journals (Sweden)

    Jhon Alberto Ochoa-Alvarez

    Full Text Available Cancer is a leading cause of death of men and women worldwide. Tumor cell motility contributes to metastatic invasion that causes the vast majority of cancer deaths. Extracellular receptors modified by α2,3-sialic acids that promote this motility can serve as ideal chemotherapeutic targets. For example, the extracellular domain of the mucin receptor podoplanin (PDPN is highly O-glycosylated with α2,3-sialic acid linked to galactose. PDPN is activated by endogenous ligands to induce tumor cell motility and metastasis. Dietary lectins that target proteins containing α2,3-sialic acid inhibit tumor cell growth. However, anti-cancer lectins that have been examined thus far target receptors that have not been identified. We report here that a lectin from the seeds of Maackia amurensis (MASL with affinity for O-linked carbohydrate chains containing sialic acid targets PDPN to inhibit transformed cell growth and motility at nanomolar concentrations. Interestingly, the biological activity of this lectin survives gastrointestinal proteolysis and enters the cardiovascular system to inhibit melanoma cell growth, migration, and tumorigenesis. These studies demonstrate how lectins may be used to help develop dietary agents that target specific receptors to combat malignant cell growth.

  19. Multiple Makerspace on University Library Service Transformation%基于众创空间的高校图书馆服务转型研究

    Institute of Scientific and Technical Information of China (English)

    陈良

    2016-01-01

    创客空间是开放式的实验平台。基于众创空间开展服务,高校图书馆应明确三大原则:以用户需求为基准,明确众创空间服务定位;制定众创空间计划,实现服务的可持续发展;加强与机构的合作,争取社会各界广泛支持。要实现服务转型,高校图书馆应实现从阅读文化到众创文化,从物理空间到虚拟空间,从信息素养到跨素养能力的转变,循序渐进开展创业型众创空间服务。还应重点解决经费和环境的问题。%Makerspace is an open experimental platform. Based on multiple makerspace,university libraries should know clearly about three major principles in conducting services:taking users’needs as the benchmark and defining a clear multiple makerspace service orientation;formulating a multiple makerspace plan to achieve a sustain﹣able development;and strengthening cooperation with agencies for community - wide support. To achieve service transformation,university libraries should change from reading culture to makerspace culture,from physical space to virtual space,and from information literacy to cross - literacy,carrying out entrepreneurial multiple makerspace serv﹣ices step by step. Attention should also be paid to solving the problems of financing and environment.

  20. Plant trait-based models identify direct and indirect effects of climate change on bundles of grassland ecosystem services.

    Science.gov (United States)

    Lamarque, Pénélope; Lavorel, Sandra; Mouchet, Maud; Quétier, Fabien

    2014-09-23

    Land use and climate change are primary causes of changes in the supply of ecosystem services (ESs). Although the consequences of climate change on ecosystem properties and associated services are well documented, the cascading impacts of climate change on ESs through changes in land use are largely overlooked. We present a trait-based framework based on an empirical model to elucidate how climate change affects tradeoffs among ESs. Using alternative scenarios for mountain grasslands, we predicted how direct effects of climate change on ecosystems and indirect effects through farmers' adaptations are likely to affect ES bundles through changes in plant functional properties. ES supply was overall more sensitive to climate than to induced management change, and ES bundles remained stable across scenarios. These responses largely reflected the restricted extent of management change in this constrained system, which was incorporated when scaling up plot level climate and management effects on ecosystem properties to the entire landscape. The trait-based approach revealed how the combination of common driving traits and common responses to changed fertility determined interactions and tradeoffs among ESs.

  1. Transformation of inorganic P fractions of soil and plant growth promotion by phosphate-solubilizing ability of Penicillium oxalicum I1.

    Science.gov (United States)

    Gong, Mingbo; Du, Peng; Liu, Xue; Zhu, Changxiong

    2014-12-01

    The solubilization of tricalcium phosphate is often considered as the standard for screening of most phosphate-solubilizing microorganisms (PSMs). However, usually the effect of large-scale application of PSM on the promotion of crop growth varies. This study presents an efficient method for screening and testing phosphate-solubilizing fungus that enhance plant growth. A fungus Penicillium oxalicum I1 (P-I1) was isolated and identified that had high ability of phosphate-solubilization and could utilize maize root exudates as sources, and propagate well in vitro and in soil. P-I1 excreted oxalic acid and reached 593.9 μg/ml, and the pH value was decreased from 6.90 to 1.65 in 26 h. The amount of P-I1 increased by 48-fold in 28 d and was maintained for 49 d in soil. PSM showed selectivity on the transformation of the different forms of phosphorus, a wide range of insoluble phosphates, such as Ca₈H₂(PO₄)₆·5H₂O, AlPO₄, FePO₄, and Ca10(PO₄)₆(OH)₂, were converted to soluble CaHPO₄in soil, and CaHPO₄was also inhibited from being converted into insoluble phosphate by P-I1. The Ca₂-P content reached 27.11 μg/g soil on day 28 at 20°C, which increased by 110.32%, and plant growth promotion was tested and verified, the results showed that maize yield increased remarkably than control after inoculated P-I1, maize yield increased maximum by 14.47%. The data presented that P-I1 appear attractive for exploring their plant growth-promoting activity and potential field application.

  2. Assessing saffron (Crocus sativus L.) adulteration with plant-derived adulterants by diffuse reflectance infrared Fourier transform spectroscopy coupled with chemometrics.

    Science.gov (United States)

    Petrakis, Eleftherios A; Polissiou, Moschos G

    2017-01-01

    Saffron, the dried red stigmas of the plant Crocus sativus L., is well-known as one of the most important and expensive spices worldwide. It is thus highly susceptible to fraudulent practices that employ, among others, plant-derived adulterants. This study presents an application of diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and chemometric techniques for evaluating adulteration of saffron with six characteristic adulterants of plant origin, i.e. C. sativus stamens, calendula, safflower, turmeric, buddleja, and gardenia. The proposed method involved a three-step process for the detection of adulteration as well as for the identification and quantification of adulterants. Partial least squares discriminant analysis (PLS-DA) was applied to perform authentication of saffron based on mid-infrared fingerprints (4000-600cm(-1)), resulting in 99% correct classification of pure saffron and saffron adulterated at 5-20% (w/w) levels. Adulterant identification in positive samples was performed with high sensitivity and specificity by a six-class PLS-DA model, with spectroscopic data from the region 2000-600cm(-1). Subsequently, partial least squares (PLS) regression models were built for the quantification of each adulterant. By using synergy interval PLS (siPLS) for variable selection, models with improved performance were developed, with detection limits ranging from 1.0% to 3.1% (w/w). The results obtained illustrate that this strategy based on DRIFTS has the potential to complement existing methodologies for the rapid and cost-effective assessment of typical saffron frauds.

  3. Model Transformations? Transformation Models!

    NARCIS (Netherlands)

    Bézivin, J.; Büttner, F.; Gogolla, M.; Jouault, F.; Kurtev, I.; Lindow, A.

    2006-01-01

    Much of the current work on model transformations seems essentially operational and executable in nature. Executable descriptions are necessary from the point of view of implementation. But from a conceptual point of view, transformations can also be viewed as descriptive models by stating only the

  4. Structural Safety Analysis Based on Seismic Service Conditions for Butterfly Valves in a Nuclear Power Plant

    Directory of Open Access Journals (Sweden)

    Sang-Uk Han

    2014-01-01

    Full Text Available The structural integrity of valves that are used to control cooling waters in the primary coolant loop that prevents boiling within the reactor in a nuclear power plant must be capable of withstanding earthquakes or other dangerous situations. In this study, numerical analyses using a finite element method, that is, static and dynamic analyses according to the rigid or flexible characteristics of the dynamic properties of a 200A butterfly valve, were performed according to the KEPIC MFA. An experimental vibration test was also carried out in order to verify the results from the modal analysis, in which a validated finite element model was obtained via a model-updating method that considers changes in the in situ experimental data. By using a validated finite element model, the equivalent static load under SSE conditions stipulated by the KEPIC MFA gave a stress of 135 MPa that occurred at the connections of the stem and body. A larger stress of 183 MPa was induced when we used a CQC method with a design response spectrum that uses 2% damping ratio. These values were lower than the allowable strength of the materials used for manufacturing the butterfly valve, and, therefore, its structural safety met the KEPIC MFA requirements.

  5. Structural safety analysis based on seismic service conditions for butterfly valves in a nuclear power plant.

    Science.gov (United States)

    Han, Sang-Uk; Ahn, Dae-Gyun; Lee, Myeong-Gon; Lee, Kwon-Hee; Han, Seung-Ho

    2014-01-01

    The structural integrity of valves that are used to control cooling waters in the primary coolant loop that prevents boiling within the reactor in a nuclear power plant must be capable of withstanding earthquakes or other dangerous situations. In this study, numerical analyses using a finite element method, that is, static and dynamic analyses according to the rigid or flexible characteristics of the dynamic properties of a 200A butterfly valve, were performed according to the KEPIC MFA. An experimental vibration test was also carried out in order to verify the results from the modal analysis, in which a validated finite element model was obtained via a model-updating method that considers changes in the in situ experimental data. By using a validated finite element model, the equivalent static load under SSE conditions stipulated by the KEPIC MFA gave a stress of 135 MPa that occurred at the connections of the stem and body. A larger stress of 183 MPa was induced when we used a CQC method with a design response spectrum that uses 2% damping ratio. These values were lower than the allowable strength of the materials used for manufacturing the butterfly valve, and, therefore, its structural safety met the KEPIC MFA requirements.

  6. Bilirubin oxidase-like proteins from Podospora anserina: promising thermostable enzymes for application in transformation of plant biomass.

    Science.gov (United States)

    Xie, Ning; Ruprich-Robert, Gwenaël; Silar, Philippe; Chapeland-Leclerc, Florence

    2015-03-01

    Plant biomass degradation by fungi is a critical step for production of biofuels, and laccases are common ligninolytic enzymes envisioned for ligninolysis. Bilirubin oxidases (BODs)-like are related to laccases, but their roles during lignocellulose degradation have not yet been fully investigated. The two BODs of the ascomycete fungus Podospora anserina were characterized by targeted gene deletions. Enzymatic assay revealed that the bod1(Δ) and bod2(Δ) mutants lost partly a thermostable laccase activity. A triple mutant inactivated for bod1, bod2 and mco, a previously investigated multicopper oxidase gene distantly related to laccases, had no thermostable laccase activity. The pattern of fruiting body production in the bod1(Δ) bod2(Δ) double mutant was changed. The bod1(Δ) and bod2(Δ) mutants were reduced in their ability to grow on ligneous and cellulosic materials. Furthermore, bod1(Δ) and bod2(Δ) mutants were defective towards resistance to phenolic substrates and H2 O2 , which may also impact lignocellulose breakdown. Double and triple mutants were more affected than single mutants, evidencing redundancy of function among BODs and mco. Overall, the data show that bod1, bod2 and mco code for non-canonical thermostable laccases that participate in the degradation of lignocellulose. Thanks to their thermal stability, these enzymes may be more promising candidate for biotechnological application than canonical laccases.

  7. 陆海选煤厂压滤系统的改造%Filter system transformation of Luhai coal preparation plant

    Institute of Scientific and Technical Information of China (English)

    李雅萍

    2016-01-01

    陆海选煤厂自投产以来,其压滤系统就存在现场卫生量大、噪音高、设备运行缺乏连动性及能源浪费等问题,我们针对现场实际情况确定了系统改造方案,其中快开压滤机反吹风系统改造从根本上降低了现场卫生污染和噪音污染问题;压滤机与运输刮板机的联锁改造杜绝了由于过多堆积物料而引起的设备堵压故障;碎饼装置改造降低了职工的劳动强度、解决了大块滤饼进入下料溜槽发生蓬仓事故的机率,消除人工疏通煤仓时存在的安全隐患;精煤刮板水煤分离的改造降低了精煤产品的水分含量,减轻了岗位司机的劳动强度;滤液水回收利用的改造充分利用了压滤机的滤液水,节约了水能源。这一系统的改造提高了人、机安全,使现场工作达到标准化要求。%Since the production of LuHai coal preparation plant ,Its pressure filtration system exsits site health capacity ,high noise ,the lack of mobility equipment operation ,waste of energy and other issues .We focus on the actual situation to determine the system transformation plan ,which fast opening filter anti‐hair system transformation reduces site health pollution and noise pollution problem fundamentally .Interlocking Reconstruction Filter and transport scraper machine eliminates equipment failure due to excessive blocking pressure caused by the accumulation of material .Broken bread device reduces the labor intensity of workers , solves the big cake into the feeding chute which occurr warehouse accident probability ,eliminates the security risks of artificial dredge coal .Coal coal‐water separation scraper transformation reduces the moisture content of the coal transformation products ,reducing the labor intensity of the driver job .The filtrate water recycling transformation make full use of the filtrate water filter press ,saves water energy .Transformation of the system improves the human

  8. 转YHem1番茄植株耐盐性的研究%Studies on Salt Tolerance in Tomato Plants by Transformation of YHem1

    Institute of Scientific and Technical Information of China (English)

    李翠; 冯新新; 张治平; 孙新娥; 汪良驹

    2012-01-01

    YHem1是一个由拟南芥HemA1启动子(一种光响应型启动子)控制的酿酒酵母菌5–氨基乙酰丙酸(ALA)合酶基因(Hem1)。将该基因转化番茄植株,可以提高叶片内源ALA含量及其代谢能力,增加叶绿素含量和抗氧化酶活性,并降低O2-产生速率和丙二醛(MDA)含量。200mmol·L-1NaCl处理,降低了野生型番茄叶片ALA合成与代谢能力和叶绿素含量,同时诱导叶片O2-产生速率、H2O2和丙二醛(MDA)含量上升,而超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)等抗氧化酶活性则逐渐降低。盐胁迫也导致转YHem1番茄ALA合成与代谢能力、叶绿素含量和抗氧化酶活性下降,但其降幅明显低于野生型。盐处理10d后,转基因番茄植株保持着较高的生物学积累量和较低的盐胁迫抑制程度,说明转入YHem1基因可以提高番茄耐盐性。此外,转基因番茄叶片H2O2含量始终保持较高水平,暗示其可能作为一种信号分子参与细胞生理调节。%YHem1 was a Saceharomyces cerevisiae Hem1 gene,controlled by an Athaliana HemA1 promoter(a light responsive promoter). When YHem1 was transformed into tomato plants,the leaf endogenous ALA content,the capacities for ALA biosynthesis and catabolism,the chlorophyll content and the antioxidant enzyme activities were all improved,while the superoxide anion(O2-)production rate and the malondialdehyde(MDA)content were depressed significantly. Treatment by 200 mmol·L-1 NaCl solution dramatically decreased the ALA biosynthetic and catabolic capacities,and the chlorophyll content in the wild type of tomato plants. It also induced increase of the O2- production rate and the H2O2 and MDA content,but inhibited the activities of superoxide dismutase(SOD),catalase(CAT),peroxidase (POD) and ascorbic peroxidase(APX). In the transgenic plants,the ALA metabolic capacities,the chlorophyll content and the

  9. 数字化与网络化环境下图书馆服务转型与服务创新%In the Digital and Networked Environment Transformation and Service Innovation of Library Service

    Institute of Scientific and Technical Information of China (English)

    唐佐琴

    2014-01-01

    The library is an important part of our teaching, research and business books, the digital process concern the industry. The author combines years of practical experience, introduced by the digitization process in the evolution of structural elements, focusing on the analysis in the digital and networked environment, library services and service innovation transformation methods for practical reference.%图书馆是我国教学、科研和图书事业的重要内容,其数字化进程备受业界人士的关注。结合笔者多年的实际经验,通过介绍数字化进程中结构要素的演变,着重分析了在数字化和网络化环境下,图书馆服务转型和服务创新的方法,以供实践参考。

  10. Evaluation of the degradation of the service water system in nuclear plants; Evaluacion de la degradacion del sistema de agua de servicio en plantas nucleares

    Energy Technology Data Exchange (ETDEWEB)

    Salaices A, E. [IIE, Av. Reforma 113, Col. Palmira, Cuernavaca, Morelos (Mexico)

    2003-07-01

    The service water system, the circulation water system, the cooling water system and the protection against fires system so much in nuclear plants as in fossils plants they are being degraded by a wide variety of mechanisms. These mechanisms include microbiologically influenced corrosion, cavitation, erosion-corrosion, erosion by solid particles, corrosion in cracks, stings, general corrosion, galvanic corrosion, sedimentation and obstructions and incrustations in the heat exchangers. In the last years were developed predictive models for the more common degradation forms and were installed in a new application of the CHECWORKS{sup TM} code called Cooling Water Application (CWA). This application of the code provides a new technology that so much nuclear facilities as fossil ones can use to modelling specific systems and to carry out corrosion predictions in each one of its components. Presently work the results of the employment of the CHECWORKS CWA code are described to carry out predictions of 12 different corrosion mechanisms that affect to the service water system of a nuclear plant, as well as the recommendations and options that the plant can to consider to reduce indexes of damages. This work can be used for to optimize inspections to the service water system and it gives the bases for similar changes in other nuclear plants. (Author)

  11. A Three-Part Electricity Price Mechanism for Photovoltaic-Battery Energy Storage Power Plants Considering the Power Quality and Ancillary Service

    Directory of Open Access Journals (Sweden)

    Yajing Gao

    2017-08-01

    Full Text Available To solve the problem of solar abandoning, which is accompanied by the rapid development of photovoltaic (PV power generation, a demonstration of a photovoltaic-battery energy storage system (PV-BESS power plant has been constructed in Qinghai province in China. However, it is difficult for the PV-BESS power plant to survive and develop with the current electricity price mechanism and subsidy policy. In this paper, a three-part electricity price mechanism is proposed based on a deep analysis of the construction and operation costs and economic income. The on-grid electricity price is divided into three parts: the capacity price, graded electricity price, and ancillary service price. First, to ensure that the investment of the PV-BESS power plant would achieve the industry benchmark income, the capacity price and benchmark electricity price are calculated using the discounted cash flow method. Then, the graded electricity price is calculated according to the grade of the quality of grid-connected power. Finally, the ancillary service price is calculated based on the graded electricity price and ancillary service compensation. The case studies verify the validity of the three-part electricity price mechanism. The verification shows that the three-part electricity price mechanism can help PV-BESS power plants to obtain good economic returns, which can promote the development of PV-BESS power plants.

  12. 基于大数据的医疗服务模式转型%Thinking on Mode Transformation of Medical Service Based on Big Data

    Institute of Scientific and Technical Information of China (English)

    方培元; 刘燕

    2016-01-01

    With the rapid development of information and technology, mass data based on the health serv-ice platform is increasing exponentially.With the development of big data technology and the implementation of“Internet +medical” strategy, high energy resources, which are comparable to oil and gold, can be extracted from the big and complex data.It is not only accelerate the medical conjecture, discovery and the transformation of the medical practice, but also derives traditional medical to be personalized and remote, to upgrade new medical serv-ice mode, such as cloud hospital.A new golden age of medical industry is coming.%信息技术的飞速发展,使各医疗卫生服务平台产生的海量数据呈指数级大爆发。大数据技术的开发应用和“互联网+医疗”战略行动的实施,能从大体量、高复杂的数据中提取堪比石油、黄金的高能资源,加速从医学猜想、发现到医疗实践的转化,催生传统医疗向个性化、远程化、云医院等新兴医疗服务模式转型升级,开拓医疗行业一个新的黄金时代。

  13. Ethylene insensitive plants

    Science.gov (United States)

    Ecker, Joseph R.; Nehring, Ramlah; McGrath, Robert B.

    2007-05-22

    Nucleic acid and polypeptide sequences are described which relate to an EIN6 gene, a gene involved in the plant ethylene response. Plant transformation vectors and transgenic plants are described which display an altered ethylene-dependent phenotype due to altered expression of EIN6 in transformed plants.

  14. Transforming a Traditional Inquiry-Based Science Unit into a STEM Unit for Elementary Pre-service Teachers: A View from the Trenches

    Science.gov (United States)

    Schmidt, Matthew; Fulton, Lori

    2016-04-01

    The need to prepare students with twenty-first-century skills through STEM-related teaching is strong, especially at the elementary level. However, most teacher education preparation programs do not focus on STEM education. In an attempt to provide an exemplary model of a STEM unit, we used a rapid prototyping approach to transform an inquiry-based unit on moon phases into one that integrated technology in a meaningful manner to develop technological literacy and scientific concepts for pre-service teachers (PSTs). Using qualitative case study methodology, we describe lessons learned related to the development and implementation of a STEM unit in an undergraduate elementary methods course, focusing on the impact the inquiry model had on PSTs' perceptions of inquiry-based science instruction and how the integration of technology impacted their learning experience. Using field notes and survey data, we uncovered three overarching themes. First, we found that PSTs held absolutist beliefs and had a need for instruction on inquiry-based learning and teaching. Second, we determined that explicit examples of effective and ineffective technology use are needed to help PSTs develop an understanding of meaningful technology integration. Finally, the rapid prototyping approach resulted in a successful modification of the unit, but caused the usability of our digital instructional materials to suffer. Our findings suggest that while inquiry-based STEM units can be implemented in existing programs, creating and testing these prototypes requires significant effort to meet PSTs' learning needs, and that iterating designs is essential to successful implementation.

  15. Midwest interstate sulfur transformation and transport project: aerial measurements of urban and power plant plumes, summer 1974. Final report, Jul 1974--Jun 1976

    Energy Technology Data Exchange (ETDEWEB)

    White, W.H.; Anderson, J.A.; Knuth, W.R.; Blumenthal, D.L.; Husar, R.B.

    1976-11-01

    A portion of the research activities of the Midwest Interstate Sulfur Transformation and Transport Project (Project MISTT) during the summer of 1974 is documented. Using a light plane equipped with instruments for measuring air pollutants and meteorological parameters, investigators mapped the three-dimensional distribution of aerosols and pollutant gases originating in the St. Louis area. Each day's flight plan was designed to characterize a large pollutant plume at discrete distances downwind from its source. The plume from the coal-fired power plant at Labadie, Missouri was followed out to 45 km. Secondary aerosol production within the plume was documented. The estimated average conversion rate for sulfur dioxide to sulfate was about three percent/hour at the distances sampled. The overall removal rate of SO/sub 2/ was too small to detect, and no net production of ozone was observed. Large pollutant plumes were also identified downwind of central St. Louis and the Wood River refineries. These urban-industrial plumes were followed out to 60-70 km, where they were characterized by elevated concentrations of ozone and light-scattering aerosols.

  16. Fourier transform infrared spectroscopic characterisation of heavy metal-induced metabolic changes in the plant-associated soil bacterium Azospirillum brasilense Sp7

    Science.gov (United States)

    Kamnev, A. A.; Antonyuk, L. P.; Tugarova, A. V.; Tarantilis, P. A.; Polissiou, M. G.; Gardiner, P. H. E.

    2002-06-01

    Structural and compositional features of whole cells of the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp7 under standard and heavy metal-stressed conditions are analysed using Fourier transform infrared (FTIR) spectroscopy and compared with the FT-Raman spectroscopic data obtained previously [J. Mol. Struct. 563-564 (2001) 199]. The structural spectroscopic information is considered together with inductively coupled plasma-mass spectrometric (ICP-MS) analytical data on the content of the heavy metal cations (Co 2+, Cu 2+ and Zn 2+) in the bacterial cells. As a bacterial response to heavy metal stress, all the three metals, being taken up by bacterial cells from the culture medium (0.2 mM) in significant amounts (ca. 0.12, 0.48 and 4.2 mg per gram of dry biomass for Co, Cu and Zn, respectively), are shown to induce essential metabolic changes in the bacterium revealed in the spectra, including the accumulation of polyester compounds in bacterial cells and their enhanced hydration affecting certain IR vibrational modes of functional groups involved.

  17. Proposal for the award of an industrial services contract for the operation and maintenance of liquid helium cryogenic plants

    CERN Document Server

    2000-01-01

    This document concerns the award of an Industrial Services contract for the operation and maintenance of liquid helium cryogenic plants. Following a market survey carried out among 54 firms in twelve Member States, a call for tenders (IT-2719/LHC) was sent on 18 August 2000 to two firms and four consortia, two consisting of two firms and two consisting of three firms, in five Member States. By the closing date, CERN had received tenders from one firm and three consortia, in four Member States. The Finance Committee is invited to agree to the negotiation of a contract with the consortium AIR LIQUIDE (FR) - LINDE KRYOTECHNIK (CH) - SERCO (DE), the lowest bidder, for an initial period of four years from 17 July 2001 for a total amount of 19 804 400 Swiss francs, not subject to revision until 16 July 2005. The contract will include options for two one-year extensions beyond the initial four-year period. The consortium has indicated the following distribution by country of the contract value covered by this adjudi...

  18. Research on plant-parasitic nematode biology conducted by the United States Department of Agriculture-Agricultural Research Service.

    Science.gov (United States)

    Chitwood, David J

    2003-01-01

    The recent de-registration of several chemical nematicides and the impending loss of methyl bromide from the pest-control market necessitate the development of new methods for controlling nematode-induced crop damage. One approach for developing novel target-specific controls is by exploiting fundamental differences between the biological processes of nematodes and their host plants. Researchers of the Agricultural Research Service (ARS) of the US Department of Agriculture are actively exploring these differences. Research accomplishments include the discovery of heat shock protein genes possibly involved in developmental arrest of the soybean cyst nematode, the identification of neuropeptides and female-specific proteins in the soybean cyst nematode, the disruption of nematode reproduction with inhibitors of nematode sterol metabolism, the development of novel morphological and molecular (heat shock protein genes and the D3 segment of large subunit ribosomal DNA) features useful for nematode identification and classification, and the elucidation of the population genetics of potato cyst nematode pathotypes. In addition, several ARS researchers are investigating biological determinants of nematode response to management strategies utilized in agricultural fields. These collective efforts should lead to new chemical and non-chemical alternatives to conventional nematode control strategies.

  19. Plant-based food and feed protein structure changes induced by gene-transformation, heating and bio-ethanol processing: a synchrotron-based molecular structure and nutrition research program.

    Science.gov (United States)

    Yu, Peiqiang

    2010-11-01

    Unlike traditional "wet" analytical methods which during processing for analysis often result in destruction or alteration of the intrinsic protein structures, advanced synchrotron radiation-based Fourier transform infrared microspectroscopy has been developed as a rapid and nondestructive and bioanalytical technique. This cutting-edge synchrotron-based bioanalytical technology, taking advantages of synchrotron light brightness (million times brighter than sun), is capable of exploring the molecular chemistry or structure of a biological tissue without destruction inherent structures at ultra-spatial resolutions. In this article, a novel approach is introduced to show the potential of the advanced synchrotron-based analytical technology, which can be used to study plant-based food or feed protein molecular structure in relation to nutrient utilization and availability. Recent progress was reported on using synchrotron-based bioanalytical technique synchrotron radiation-based Fourier transform infrared microspectroscopy and diffused reflectance infrared Fourier transform spectroscopy to detect the effects of gene-transformation (Application 1), autoclaving (Application 2), and bio-ethanol processing (Application 3) on plant-based food and feed protein structure changes on a molecular basis. The synchrotron-based technology provides a new approach for plant-based protein structure research at ultra-spatial resolutions at cellular and molecular levels.

  20. Efficacy of using multiple open-path Fourier transform infrared (OP-FTIR) spectrometers in an odor emission episode investigation at a semiconductor manufacturing plant.

    Science.gov (United States)

    Tsao, Yung-Chieh; Wu, Chang-Fu; Chang, Pao-Erh; Chen, Shin-Yu; Hwang, Yaw-Huei

    2011-08-01

    This study evaluated the efficacy of simultaneously employing three open-path Fourier transform infrared (OP-FTIR) spectrometers with 3-day consecutive monitoring, using an odor episode as an example. The corresponding monitoring paths were allocated among the possible emission sources of a semiconductor manufacturing plant and the surrounding optoelectronic and electronic-related factories, which were located in a high-tech industrial park. There was a combined total odor rate of 43.9% for the three monitoring paths, each comprised of 736 continuous 5-minute monitoring records and containing detectable odor compounds, such as ammonia, ozone, butyl acetate, and propylene glycol monomethyl ether acetate (PGMEA). The results of the logistic regression model indicated that the prevailing south wind and the OP-FTIR monitoring path closest to the emission source in down-wind direction resulted in a high efficacy for detecting odorous samples with odds ratios (OR) of 3.8 (95% confidence interval (CI): 2.9-5.0) and 5.1 (95% CI: 3.6-7.2), respectively. Meanwhile, the odds ratio for detecting ammonia odorous samples was 7.5 for Path II, which was downwind closer to the possible source, as compared to Path III, downwind far away from the possible source. PGMEA could not be monitored at Path II but could be at Path III, indicating the importance of the monitoring path and flow ejection velocities inside the stacks on the monitoring performance of OP-FTIR. Besides, an odds ratio of 5.1 for odorous sample detection was obtained with south prevailing wind comprising 65.0% of the monitoring time period. In general, it is concluded that OP-FTIR operated with multiple paths simultaneously shall be considered for investigation on relatively complicated episodes such as emergency of chemical release, multiple-source emission and chemical monitoring for odor in a densely populated plant area to enhance the efficacy of OP-FTIR monitoring. Copyright © 2011 Elsevier B.V. All rights

  1. Plastid transformation in eggplant.

    Science.gov (United States)

    Bansal, Kailash C; Singh, Ajay K

    2014-01-01

    Eggplant (Solanum melongena L.) is an important vegetable crop of tropical and temperate regions of the world. Here we describe a procedure for eggplant plastid transformation, which involves preparation of explants, biolistic delivery of plastid transformation vector into green stem segments, selection procedure, and identification of the transplastomic plants. Shoot buds appear from cut ends of the stem explants following 5-6 weeks of spectinomycin selection after bombardment with the plastid transformation vector containing aadA gene as selectable marker. Transplastomic lines are obtained after the regenerated shoots are subjected to several rounds of spectinomycin selection over a period of 9 weeks. Homoplasmic transplastomic lines are further confirmed by spectinomycin and streptomycin double selection. The transplastomic technology development in this plant species will open up exciting possibilities for improving crop performance, metabolic engineering, and the use of plants as factories for producing biopharmaceuticals.

  2. Biofiltration and electrochemical treatment for the production of service water from outflows of small-scaled sewage treatment plants; Biofiltration und elektrochemische Behandlung zur Brauchwassererzeugung aus Kleinklaeranlagenablaeufen

    Energy Technology Data Exchange (ETDEWEB)

    Ilian, Jens

    2010-12-14

    Up to the 1990s a mechanical partly biological wastewater treatment was performed at remote locations or collected in reservoirs without outflow. The currently valid legal regulations require a biological treatment of wastewater. Thus, biological small-scale sewage treatment plants experience a broad dissemination recently. Under this aspect, the author of the contribution under consideration reports on the bio filtration and electrochemical treatment in order to produce service water from outflows of small-scale sewage treatment plants. The author investigates the legal regulations, and supplements these regulations by own definitions and requirements on the consideration of a hygienic potential for damage. Additionally, investigations on the cleaning performance of properly operated small-scaled sewage treatment plants are performed. The hygienic risk potential as an inflow condition of a disinfection is determined.

  3. Genetic Transformation of Switchgrass

    Science.gov (United States)

    Xi, Yajun; Ge, Yaxin; Wang, Zeng-Yu

    Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced from caryopses or inflorescences were used as explants for inoculation with A. tumefaciens strain EHA105. Hygromycin phosphotransferase gene (hph) was used as the selectable marker and hygromycin was used as the selection agent. Calluses resistant to hygromycin were obtained after 5-6 weeks of selection. Soil-grown switchgrass plants were regenerated about 6 months after callus induction and Agrobacterium-mediated transformation.

  4. 磁暴对沿海核电站变压器安全影响的研究%Study on the Impact of Magnetic Storm on Security of Transformers in Coastal Nuclear Plants

    Institute of Scientific and Technical Information of China (English)

    刘连光; 原丽; 王智冬; 郑宽; 冯学尚

    2011-01-01

    The geomagnetically induced current (CIC) caused by magnetic storm would damage transformers directly and disturb relay protections or some other equipments indirectly, and strong magnetic storm could threaten the security of power system. For verifying the magnetic storm impact on the transformers in coastal nuclear plants, a mass of GIC data was collected and statistically analyzed, which included the measured GIC data in Guangdong Lingao nuclear power plant and Jiangsu Shanghe substation and the calculated GIC data in Northwest 750 kV power grid ( all the data above happened at the peak of 23rd solar cycle during the strong magnetic storm). The differences of the transformer's GIC between coastal nuclear power plant and inland substation and their reasons were mainly studied. The result indicates that the transformers in coastal nuclear power plants are more vulnerable to the magnetic storm impact due to the influence of the coastal effect. On this basis, the possible impacts and risks of extreme magnetic storm on the large-scale power system are assessed preliminarily, and it is suggested that the impact of magnetic storm should be assessed while selecting nuclear power plants location and transformers type.%磁暴产生的地磁感应电流(GIC)直接损伤变压器和间接干扰继电保护等设备,强磁暴可能威胁到电网的安全运行.为探明磁暴对沿海核电站变压器的影响,对第23太阳周峰年强磁暴侵害广东岭澳核电站、江苏上河变电站的GIC测量数据,以及西北陕甘青宁750 kV电网计算数据进行统计分析,研究沿海核电站与内陆变电站变压器GIC大小及其差异原因.结果表明,由于受海岸效应现象的影响,沿海核电站的变压器更容易遭受磁暴的侵害.在此基础上,对极端磁暴的可能危害、灾害风险以及沿海核电站的磁暴灾害进行评估,并建议核电站选址和变压器选型时应评估磁暴的影响.

  5. 6MW Back Pressure Unit Technical Transformation in a Thermal Power Plant%某热电厂6 MW背压机组技术改造

    Institute of Scientific and Technical Information of China (English)

    郭峰; 胡庆兵; 何鹏

    2015-01-01

    To improve the utilization rate of energy, a thermal power plant planned to build a 6MW medi-um temperature sub-high pressure back pressure turbine generator unit, which would replace two 3MW medium temperature and medium pressure back pressure turbine generator units. At the same time, a 3 MW extraction condensing unit in the first phase of the project was converted into a back pressure tur-bine with post machine connected with the exhaust steam pipe in order to solve the problem of poor system stability caused by the low heat load in the off-season in the company. It used the exhaust steam of the back pressure turbine as its steam admission to maintain the stable operation of the back pressure machine. This paper analyzed the technical transformation. Using the back pressure turbine with high parameters instead of the one with low parameters can improve energy efficiency; reduce pollutant emis-sions to achieve the aim of saving energy, protecting the environment and enhancing the area resident’ s quality of life.%某热电厂为提高能源利用率,拟新建一台6 MW中温次高压背压式汽轮发电机组,替代2台3 MW中温中压背压式汽轮发电机组,同时为解决公司淡季热负荷低造成系统稳定性差的问题,将一期1台3 MW抽凝机组改造成后置机接在背压机排汽管道后,利用背压机排汽作为它的进汽,起到维持背压机稳定运行的作用。本文对此技术改造进行了分析,以高参数的背压机组替代低参数的背压机组,提高能源利用率,减少污染物排放总量,达到节约能源、保护环境、提高区域内居民生活质量的目的。

  6. Identification of microplastic in effluents of waste water treatment plants using focal plane array-based micro-Fourier-transform infrared imaging.

    Science.gov (United States)

    Mintenig, S M; Int-Veen, I; Löder, M G J; Primpke, S; Gerdts, G

    2017-01-01

    The global presence of microplastic (MP) in aquatic ecosystems has been shown by various studies. However, neither MP concentrations nor their sources or sinks are completely known. Waste water treatment plants (WWTPs) are considered as significant point sources discharging MP to the environment. This study investigated MP in the effluents of 12 WWTPs in Lower Saxony, Germany. Samples were purified by a plastic-preserving enzymatic-oxidative procedure and subsequent density separation using a zinc chloride solution. For analysis, attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FT-IR) and focal plane array (FPA)-based transmission micro-FT-IR imaging were applied. This allowed the identification of polymers of all MP down to a size of 20 μm. In all effluents MP was found with quantities ranging from 0 to 5 × 10(1) m(-3) MP > 500 μm and 1 × 10(1) to 9 × 10(3) m(-3) MP < 500 μm. By far, polyethylene was the most frequent polymer type in both size classes. Quantities of synthetic fibres ranged from 9 × 10(1) to 1 × 10(3) m(-3) and were predominantly made of polyester. Considering the annual effluxes of tested WWTPs, total discharges of 9 × 10(7) to 4 × 10(9) MP particles and fibres per WWTP could be expected. Interestingly, one tertiary WWTP had an additionally installed post-filtration that reduced the total MP discharge by 97%. Furthermore, the sewage sludge of six WWTPs was examined and the existence of MP, predominantly polyethylene, revealed. Our findings suggest that WWTPs could be a sink but also a source of MP and thus can be considered to play an important role for environmental MP pollution. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  7. Elimination of micropollutants and transformation products from a wastewater treatment plant effluent through pilot scale ozonation followed by various activated carbon and biological filters.

    Science.gov (United States)

    Knopp, Gregor; Prasse, Carsten; Ternes, Thomas A; Cornel, Peter

    2016-09-01

    Conventional wastewater treatment plants are ineffective in removing a broad range of micropollutants, resulting in the release of these compounds into the aquatic environment, including natural drinking water resources. Ozonation is a suitable treatment process for micropollutant removal, although, currently, little is known about the formation, behavior, and removal of transformation products (TP) formed during ozonation. We investigated the elimination of 30 selected micropollutants (pharmaceuticals, X-ray contrast media, industrial chemicals, and TP) by biological treatment coupled with ozonation and, subsequently, in parallel with two biological filters (BF) or granular activated carbon (GAC) filters. The selected micropollutants were removed to very different extents during the conventional biological wastewater treatment process. Ozonation (specific ozone consumption: 0.87 ± 0.29 gO3 gDOC(-1), hydraulic retention time: 17 ± 3 min) eliminated a large number of the investigated micropollutants. Although 11 micropollutants could still be detected after ozonation, most of these were eliminated in subsequent GAC filtration at bed volumes (BV) of approximately 25,000 m(3) m(-3). In contrast, no additional removal of micropollutants was achieved in the BF. Ozonation of the analgesic tramadol led to the formation of tramadol-N-oxide that is effectively eliminated by GAC filters, but not by BF. For the antiviral drug acyclovir, the formation of carboxy-acyclovir was observed during activated sludge treatment, with an average concentration of 3.4 ± 1.4 μg L(-1) detected in effluent samples. Subsequent ozonation resulted in the complete elimination of carboxy-acyclovir and led to the formation of N-(4-carbamoyl-2-imino-5-oxo imidazolidin)-formamido-N-methoxyacetetic acid (COFA; average concentration: 2.6 ± 1.0 μg L(-1)). Neither the BF nor the GAC filters were able to remove COFA. These results highlight the importance of considering TP in the

  8. A Research of Service Transformation Strategy of Equipment Manufacturing Industry in Heilongjiang Province%黑龙江省装备制造业服务化转型策略研究

    Institute of Scientific and Technical Information of China (English)

    李知渊; 蔡野

    2016-01-01

    伴随市场环境不断变化,装备制造业服务化转型已成为提升其核心竞争力的必然选择,本文基于黑龙江省装备制造业发展现状展开研究,从产业集群低、自主创新能力差、商业模式单一等方面系统分析制约黑龙江省装备制造业服务化转型的主要因素,在此基础上针对性的提出加快黑龙江省装备制造业服务化转型的策略建议。本文的研究成果可为黑龙江省装备制造业的服务化转型及产业发展提供一定的理论指导。%With the constantly changing market conditions, the service transformation of equipment manufacturing industry has become the necessity of boosting its core competiveness. According to the current situation of equipment manufacturing industry in Heilongjiang, the thesis analyzes main factors restricting the trans-formation of equipment manufacturing industry, such as the low industry cluster, poor initiative, and single business model. Based on the above-mentioned aspects, the thesis offers strategic sugges-tions of hastening the service transformation of equipment manufac-turing industry in Heilongjiang. The thesis can be used as theoret-ical guidance for the service transformation of equipment manufac-turing industry in Heilongjiang and its industry development.

  9. Service Transformation and Innovation of University Library under the Circumstance of Information Society%信息社会环境下高校图书馆服务转型和创新

    Institute of Scientific and Technical Information of China (English)

    陈红

    2015-01-01

    信息社会环境下网络化、数字化和现代信息技术的发展使高校图书馆服务发生了巨大变化,本文重点分析了高校图书馆在服务理念、馆藏结构、服务模式、服务方式、服务内容等几个方面进行创新式转型,并具体讨论了高校图书馆服务创新的举措。%Under the circumstance of information society, the development of digitalization, networking and modern information technology has made great changes in the service of university library, this paper focused on the innovative transformation of university library in service concept, collection structure, service mode, service method, service contents, and discussed the specific measures of university library service innovation.

  10. ADJUSTMENT, MAINTENANCE, AND REPAIR OF TILLAGE, PLANTING, SPRAYING, AND FERTILIZING MACHINERY. AGRICULTURAL MACHINERY--SERVICE OCCUPATIONS, MODULE NUMBER 10.

    Science.gov (United States)

    Ohio State Univ., Columbus. Center for Vocational and Technical Education.

    THE PURPOSE OF THIS GUIDE IS TO HELP TEACHERS PREPARE POSTSECONDARY-LEVEL STUDENTS FOR THE AGRICULTURAL MACHINERY SERVICE OCCUPATIONS AS PARTS MEN, MECHANICS, MECHANIC'S HELPERS, AND SERVICE SUPERVISORS. IT WAS DESIGNED BY A NATIONAL TASK FORCE ON THE BASIS OF RESEARCH FROM STATE STUDIES. THE MAJOR OBJECTIVE IS TO DEVELOP (1) STUDENT UNDERSTANDING…

  11. Examining the use of color-infrared digital aerial photography to map common non-native invasive plants on two Southern Indiana U.S. Fish & Wildlife Service National Wildlife Refuges

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — OBJECTIVES: The overall objective was to determine if CIR digital aerial photography could be used to efficiently map and analyze common invasive plant distribution...

  12. Core Socialist Values to Guide Volunteer Service Transformed from Selfish to Altruistic%社会主义核心价值观引导志愿服务由利己向利他转化

    Institute of Scientific and Technical Information of China (English)

    李辉容; 叶飞霞

    2015-01-01

    如何实现工具理性的"利己"向价值理性的"利他"转化是志愿服务过程中面临的一个问题,二者之间的转化关系到志愿精神的实现程度. 社会主义核心价值观倡导社会价值与个人价值的统一,是志愿服务由利己性动机向利他性动机转化的内在驱动力;志愿服务在实现利己过程中并不排斥利他性行为的实现,存在着转化可能.因此,应该创新举措,促进志愿服务在社会主义核心价值观引导下实现由利己向利他转化.%It is a question to transform instrumental rationality"selfish"to value rational"altruism"in the course of volunteer service.It concerns the degree of realization about volunteer spirit.The core socialist values propose uni-fied social values and personal values.It is the internal driving force of the volunteer service conversing from the self-serving motivation to altruistic motivation.The volunteer service does not exclude the implementation of altruistic be-havior in the process of realization of self-interest, and there is a possible to transform it.Thus,we should make the core socialist values realize the transformation of volunteer service.

  13. Agrobacterium-mediated transformation: rice transformation.

    Science.gov (United States)

    Slamet-Loedin, Inez H; Chadha-Mohanty, Prabhjit; Torrizo, Lina

    2014-01-01

    Agrobacterium is a common soil bacterium with natural capacity for trans-kingdom transfer of genetic information by transferring its T-DNA into the eukaryotic genome. In agricultural plant biotechnology, combination of non-phytopathogenic strain of Agrobacterium tumefaciens with modified T-DNA and vir-genes in a binary vector system is the most widely utilized system for genetic improvement in diverse plant species and for gene function validation. Here we have described a highly efficient A. tumefaciens-mediated transformation system for indica and japonica rice cultivars based on an immature embryo system.

  14. Sewerage Treatment Plants, Published in Not Provided, 1:4800 (1in=400ft) scale, Warren County Emergency Services.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Sewerage Treatment Plants dataset, published at 1:4800 (1in=400ft) scale, was produced all or in part from Hardcopy Maps information as of Not Provided. Data...

  15. Water Treatment Plants, Published in Not Provided, 1:4800 (1in=400ft) scale, Warren County Emergency Services.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Water Treatment Plants dataset, published at 1:4800 (1in=400ft) scale, was produced all or in part from Hardcopy Maps information as of Not Provided. Data by...

  16. Seed planting

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes prairie seed plantings on Neal Smith National Wildlife Refuge (formerly Walnut Creek National Wildlife Refuge) between 1992 and 2009.

  17. 发电厂主变压器直流偏磁原因分析%Cause Analysis of Direct Current Magnetic Bias of 500 kV Main Transformer in Power Plant

    Institute of Scientific and Technical Information of China (English)

    车传强; 郭金刚; 杨东其

    2015-01-01

    In a region of Inner Mongolia, ± 500 kV dc transmission line under the condition of monopole operation mode and taking the earth as a loop, DC magnetic bias occured in main transformer 2 in near E power plant. The test to Dc current, noise and vibration of the main transformer 2 showed that the DC current in transformer neutral point, noise and vibration exceeded the standard requirements, which has disadvantageous affect on the normal operation of transformer. As well as technical and economic aspects of the various solutions DC bias of schemes are compared, it is recommended blocking capacitor method for two main transformer neutral point for technological innovation, to ensure the safe and stable operation of the transformer.%内蒙古某地区±500 kV直流输电线路在单极运行方式并采用大地作为回线的情况下,附近E发电厂内运行的2号主变压器产生严重的直流偏磁现象.通过测试2号主变压器的直流电流、噪声、振动情况,发现主变压器中性点直流电流、噪声、振动均超过标准要求,影响变压器的正常运行.从现场实际应用效果和技术、经济性等方面对各种解决方案进行分析对比,建议采用电容隔直法对2台主变压器中性点进行技术改造,保证变压器安全稳定运行.

  18. Advancing Crop Transformation in the Era of Genome Editing

    Science.gov (United States)

    Plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. Unfortunately, for most crops, transformation and regeneration remain arduous even after more than thirty years of technological advances. Genome editing provides new opportunities to...

  19. 计算机监控系统改造在观音阁水库电厂的应用%Application of Computer Monitoring System Transformation in Guanyinge Reservoir Power Plant

    Institute of Scientific and Technical Information of China (English)

    王金华

    2014-01-01

    为全面提高观音阁水库电厂设备的安全运行和发电质量,观音阁水库电厂经过10多年的运行管理,与时俱进,不断完善计算机监控系统及其设备更新改造,目前设备运行良好,已显现出了计算机监控系统自动化改造的优越性。本文对计算机监控系统自动化改造在观音阁水库电厂的应用进行论述。%Guanyinge Reservoir Water Plant is operated and managed for more than 10 years,and the computer monitoring system and equipment updating renovation are constantly improved with the times in order to comprehensively improve the safe operation and power generation quality of equipment in Guanyinge Reservoir Water Plant.The equipment is currently operated well,and the superiority of computer monitoring system automation transformation has been shown.In this paper, the application of computer monitoring system automation transformation in Guanyinge Reservoir Water Plant is discussed.

  20. The integration of solar power plants for domestic water services in buildings; Integracion de calderas y calentadores individuales en las instalaciones de ACS con energia solar

    Energy Technology Data Exchange (ETDEWEB)

    Martin, J. V.; Garcia, R.; Lopez de Subijana, R.; Casado, J. M.

    2004-07-01

    The integration of solar power plants for domestic water services in buildings with individual heating and domestic water facilities has some problems which must be solved by the manufacturers of boilers and individual heaters; the most important is the water temperature in the entrance to the individual equipment because of solar heating. Therefore, we must care about materials in boilers and heaters, and temperature control systems of domestic water production. We analyse the technical conditions which appear in these equipment in the usual schemes: Centralized accumulation, distributed accumulation, serial heating, etc., and propose some elements which can be integrated in these facilities to obtain a better operation. (Author)

  1. Is an ecosystem services-based approach developed for setting specific protection goals for plant protection products applicable to other chemicals?

    Science.gov (United States)

    Maltby, Lorraine; Jackson, Mathew; Whale, Graham; Brown, A Ross; Hamer, Mick; Solga, Andreas; Kabouw, Patrick; Woods, Richard; Marshall, Stuart

    2017-02-15

    Clearly defined protection goals specifying what to protect, where and when, are required for designing scientifically sound risk assessments and effective risk management of chemicals. Environmental protection goals specified in EU legislation are defined in general terms, resulting in uncertainty in how to achieve them. In 2010, the European Food Safety Authority (EFSA) published a framework to identify more specific protection goals based on ecosystem services potentially affected by plant protection products. But how applicable is this framework to chemicals with different emission scenarios and receptor ecosystems? Four case studies used to address this question were: (i) oil refinery waste water exposure in estuarine environments; (ii) oil dispersant exposure in aquatic environments; (iii) down the drain chemicals exposure in a wide range of ecosystems (terrestrial and aquatic); (iv) persistent organic pollutant exposure in remote (pristine) Arctic environments. A four-step process was followed to identify ecosystems and services potentially impacted by chemical emissions and to define specific protection goals. Case studies demonstrated that, in principle, the ecosystem services concept and the EFSA framework can be applied to derive specific protection goals for a broad range of chemical exposure scenarios. By identifying key habitats and ecosystem services of concern, the approach offers the potential for greater spatial and temporal resolution, together with increased environmental relevance, in chemical risk assessments. With modifications including improved clarity on terminology/definitions and further development/refinement of the key concepts, we believe the principles of the EFSA framework could provide a methodical approach to the identification and prioritization of ecosystems, ecosystem services and the service providing units that are most at risk from chemical exposure.

  2. To Promote Open Universities Transformation Based on the Concept of Government Purchasing Education Service%以政府购买服务的理念推进开放大学转型发展

    Institute of Scientific and Technical Information of China (English)

    胡鹏

    2015-01-01

    建设开放大学是国家教育体制改革和制度创新的重大试点项目,是构建国家终身教育体系、建设人力资源强国的一项重要战略举措。政府购买教育服务不仅是政府职能转变的重要体现,更重要的是一项社会治理机制创新,以政府购买服务的理念促进地方开放大学转型发展,是一种基于机制创新的积极尝试。近年来,常州市政府着眼于开放大学的非学历教育服务,开展了以政府购买服务,促进常州开放大学转型发展的探索与实践,并从认识、实施条件、过程管理和政策支持等方面进行了反思。%The construction of open universities is a key pilot project of the reform and innovation of the national educational sys-tem and an important strategy of developing a lifelong education system and building a strong country with rich human resources. Government purchasing education service is not only an important symbol of the government functions transformation but also an innovation of public administration system.To promote the local open universities transformation according to the concept of gov-ernment purchasing education service is an active trial of mechanical innovation.In recent years,the municipal government of Changzhou,focusing on non-degree education service of open universities,has taken the initiative to explore on how to promote the open universities transformation and development with the help of government purchasing education service.Some policies and proposals are put forward for the relevant problems in the course of transformation.

  3. Role of eucalypt and other planted forests in biodiversity conservation and the provision of biodiversity-related services

    Science.gov (United States)

    Eckehard G. Brockerhoff; Hervé Jactel; John A. Parrotta; Silvio F.B. Ferraz

    2013-01-01

    Forests provide important habitat for much of the world’s biodiversity, and the continuing global deforestation is one of our greatest environmental concerns. Planted forests represent an increasing proportion of the global forest area and partly compensate for the loss of natural forest in terms of forest area, habitat for biodiversity and ecological function. At...

  4. An ecosystem services approach to pesticide risk assessment and risk management of non-target terrestrial plants: recommendations from a SETAC Europe workshop.

    Science.gov (United States)

    Arts, Gertie H P; Dollinger, Margit; Kohlschmid, Eva; Maltby, Lorraine; Ochoa-Acuña, Hugo; Poulsen, Véronique

    2015-02-01

    The registration of plant protection products (PPPs) in the EU is under Regulation 1107/2009, which recommends a tiered approach to assessing the risk to non-target terrestrial plants (NTTPs). However, little information is provided on how to perform and implement higher tier studies or how to use them to refine the risk assessments. Therefore, a stakeholder workshop was organized to consolidate current knowledge and expertise to aid the further development of testing and assessment procedures for NTTPs. This brief communication highlights the agreed recommendations of the workshop, which relate to the three main themes, i.e. specific protection goals, risk assessment and mitigation. The participants of the workshop adopted the European Food Safety Authority (EFSA) approach of using an ecosystem services framework for identifying specific protection goals. First, delivery and protection of ecosystem services were discussed for in-crop, in-field and off-crop, and off-field areas. Second, lower and higher tier risk assessment methods, including modelling approaches, were evaluated. Third, options for risk mitigation of spray drift and run-off were discussed and evaluated. Several important knowledge gaps were identified, and specific data collation and literature-based tasks were actioned to begin to address them. A full workshop report is planned for the fall of 2014.

  5. Development of Transgenic Maize Plants Tolerant to Glyphosate via Pollen-mediated Transformation and Their Glyphosate Tolerance%花粉介导法获得耐草甘膦玉米植株及其耐性研究

    Institute of Scientific and Technical Information of China (English)

    杨慧珍; 任志强; 肖建红; 卜华虎; 刘惠民

    2016-01-01

    为获得耐草甘膦转基因玉米植株,进而研究其除草剂耐性及外源基因的遗传特性,以质粒 pBI101-aroA-M12为外源基因供体,以玉米自交系昌‘7-2’为受体,采用花粉介导法将外源基因导入玉米自交系中。PCR 扩增、Southern 杂交分析的结果证明获得了54个转基因植株;对其后代株系的分子检测及田间生物学鉴定,结果证明目的基因可以稳定遗传给后代植株,且赋予和提高了转基因植株的除草剂耐性。目的基因在转化体中的遗传规律研究,结果显示导入的目的基因在 F2受体植株中以3∶1的比例发生遗传分离,符合孟德尔单因子显性遗传分离规律。ELISA 分析和蛋白质浓度测定的结果证实目的基因在转化植株中得到表达,表达量介于40.5-112.6 ng/g 叶片鲜重之间,目的基因表达量与该植株的除草剂耐性性状间呈极显著相关,r=0.9423(P<0.01)。最终研究获得了4个高草甘膦耐性的转基因纯合株系。%In order to develop transgenic maize plants tolerant to glyphosate and further study their herbicide tolerance and the genetic characteristics of exogenous gene in transgenic plants,plasmid pBI101-aroA-M12 harboring aroA-M12 gene was transformed into maize inbred Chang‘7-2’plants by pollen-mediated transformation method. Fifty-four transgenic plants were obtained by PCR detection and Southern blot analysis. The detection results of molecular analysis and bioassay in the field showed that target aroA-M12 gene was integrated into the genome of transgenic plants and could stably inherit to next generation. Moreover,the aroA-M12 gene conferred the glyphosate-tolerance to transgenic plants. The result of genetic rule of target gene in transgenic plants suggested that the target gene was segregated according to the ratio of 3∶1 in T2 generation transgenic plants(lines)and it was consistent with the Mendel law of single-factor heredity. The results

  6. 制造企业服务化转型影响因素扎根研究%Research on the Influence Factors of Manufacturers Service Transformation Based on the Grounded Theory

    Institute of Scientific and Technical Information of China (English)

    杨水利; 梁永康

    2016-01-01

    Based on 16 typical cases ,this study analyzed the influence factors of manufacturers service transformation and the mechanism by grounded theory approach .The results found 15 main factors which belongs to the seven major catego-ries :the role of industrial chain ,strategic planning ,leadership ability ,service resource integration capabilities ,technolo-gy innovation capabilities ,technical service capabilities ,business model innovation .At the same time ,the study construc-ted the theoretical model from the "decision abilities mode" and interpreted the mechanism of action .This research will help manufacturers understand the restricting factors of service transformation more clearly and provide theoretical support for servitization .%以16个服务化转型的制造企业案例为样本,运用扎根理论研究方法,深入挖掘制造企业服务化转型影响因素.研究发现了15项主要影响因素,类属于7大主范畴,包括产业链角色、战略规划、领导者能力、资源整合能力、技术创新能力、技术服务能力、商业模式创新.从"决策—能力—模式"3个层面构建作用机理模型,阐释了各因素对企业服务化转型的作用过程.研究结论有助于制造企业厘清服务化转型的影响因素,为企业转型提供理论支持.

  7. The concept of extending the service life of the VVER-440-based power units at the Novovoronezh nuclear power plant

    Science.gov (United States)

    Asmolov, V. G.; Povarov, V. P.; Vitkovskii, S. L.; Berkovich, V. Ya.; Chetverikov, A. E.; Mozul', I. A.; Semchenkov, Yu. M.; Suslov, A. I.

    2014-02-01

    Basic statements of the Concept of Extending the Service Life of the VVER-440-Based Power Units at the Novovoronezh NPP beyond 45 years are considered. This topic is raised in connection with the fact that that in December 2016 and in December 2017 the extended service lives of Units 3 and 4 at this NPP will expire. The adopted concept of repeatedly extending the service life of the Novovoronezh NPP Unit 4 implies fitting the power unit with additional reactor core cooling systems with a view to extend the (ultimate) design-basis accidents (which have hitherto been adopted to be a loss of coolant accident involving a leak of reactor coolant through a break with a nominal diameter of 100 mm) to a reactor coolant leak equivalent to rupture of the main reactor coolant pipeline. The modified Unit 4 will also use the safety systems of Unit 3 that is going to be decommissioned. Preliminary calculated assessments of the new design-basis accident scenario involving rupture of the reactor coolant pipeline in Unit 4 fitted with a new configuration of safety systems confirmed the correctness of the adopted concept of repeatedly extending the service life of Unit 4.

  8. Macroclimatic change expected to transform coastal wetland ecosystems this century

    Science.gov (United States)

    Gabler, Christopher A.; Osland, Michael J.; Grace, James B.; Stagg, Camille L.; Day, Richard H.; Hartley, Stephen B.; Enwright, Nicholas M.; From, Andrew; McCoy, Meagan L.; McLeod, Jennie L.

    2017-01-01

    Coastal wetlands, existing at the interface between land and sea, are highly vulnerable to climate change. Macroclimate (for example, temperature and precipitation regimes) greatly influences coastal wetland ecosystem structure and function. However, research on climate change impacts in coastal wetlands has concentrated primarily on sea-level rise and largely ignored macroclimatic drivers, despite their power to transform plant community structure and modify ecosystem goods and services. Here, we model wetland plant community structure based on macroclimate using field data collected across broad temperature and precipitation gradients along the northern Gulf of Mexico coast. Our analyses quantify strongly nonlinear temperature thresholds regulating the potential for marsh-to-mangrove conversion. We also identify precipitation thresholds for dominance by various functional groups, including succulent plants and unvegetated mudflats. Macroclimate-driven shifts in foundation plant species abundance will have large effects on certain ecosystem goods and services. Based on current and projected climatic conditions, we project that transformative ecological changes are probable throughout the region this century, even under conservative climate scenarios. Coastal wetland ecosystems are functionally similar worldwide, so changes in this region are indicative of potential future changes in climatically similar regions globally.

  9. Hadamard Transforms

    CERN Document Server

    Agaian, Sos; Egiazarian, Karen; Astola, Jaakko

    2011-01-01

    The Hadamard matrix and Hadamard transform are fundamental problem-solving tools in a wide spectrum of scientific disciplines and technologies, such as communication systems, signal and image processing (signal representation, coding, filtering, recognition, and watermarking), digital logic (Boolean function analysis and synthesis), and fault-tolerant system design. Hadamard Transforms intends to bring together different topics concerning current developments in Hadamard matrices, transforms, and their applications. Each chapter begins with the basics of the theory, progresses to more advanced

  10. The Potential Role of New Technology for Enhanced Safety and Performance of Nuclear Power Plants Through Improved Service Maintenance

    Science.gov (United States)

    1991-06-01

    Analysis of Severe Core Damage Potential Nureg .1 150 and Nureg /CR 4550 (N-3,4,5,6). (2) Status reports on precursors to potential severe core damage...power plant PRAs, 14 of which are PWRs (G-1.2). A severe accident risks assessment report ( Nureg 1150), issued by the Nuclear Regulatory Commission...NRC) in June of 1989, summarized the Analysis of Core Damage Reports ( Nureg /CR-4550) for five light water reactors (LWR) along with other inputs. Nureg

  11. 益阳电厂300MW机组排粉风机节能改造研究%Research on Yiyang Power Plant 300 MW unit powder exhaust fan energy-saving transformation

    Institute of Scientific and Technical Information of China (English)

    左宁心; 舒展; 王良超

    2011-01-01

    为了促进常规火力发电企业的可持续发展,降低机组运行的能耗指标,结合益阳电厂2×300 MW机组排粉风机节能改造问题,以排粉风机各参数测试数据为基础,通过对风机选型计算、电机功率匹配分析等方面的研究,提出了以火力发电厂离心式风机节能改造的一种方法,实测数据表明本文所述方法节能效果明显.%In order to promote the sustainable development of conventional thermal power generation companies, the Energy Consumption Index of the unit operation should be reduced. The paper studied the energy saving transformation of Yiyang Power Plant 2 × 300 MW units Exhaust Fan. Based on the testing data of various parameters of Powder Exhaust Fan, and the research on the calculation of fan selection and the motor power matching analysis, a method of energy saving transformation of thermal power plant centrifugal fan was proposed for reference. The measurement data indicates that the method can remarkably save the energy as described in this article.

  12. Advances in Agrobacterium-mediated plant transformation with enphasys on soybean Avanços na transformação genética de plantas mediada por Agrobacterium com ênfase em soja

    Directory of Open Access Journals (Sweden)

    Paulo Celso de Mello-Farias

    2008-02-01

    Full Text Available Soybean is one of humanity's major sources of plant protein. It is also very important for animal feed and as industrial raw material. Great advances have recently been achieved in its genetic transformation. This review provides a comprehensive discussion of important factors affecting Agrobacterium-mediated soybean transformation including target tissues, plant tissue health, wounding methods, regeneration systems, selectable markers and reporter genes.A soja é uma das maiores fontes de proteína vegetal da humanidade. É também muito importante na alimentação animal e como matéria-prima industrial. Grandes avanços foram recentemente alcançados em sua transformação genética. O objetivo desta revisão é fornecer uma discussão acerca de importantes fatores que influenciam a transformação de soja mediada por Agrobaterium, incluindo tecidos alvo, condições do tecido vegetal, métodos de injúria, sistemas de regeneração, marcadores de seleção e genes repórter.

  13. Plants having modified response to ethylene

    Science.gov (United States)

    Meyerowitz, E.M.; Chang, C.; Bleecker, A.B.

    1997-11-18

    The invention includes transformed plants having at least one cell transformed with a modified ETR nucleic acid. Such plants have a phenotype characterized by a decrease in the response of at least one transformed plant cell to ethylene as compared to a plant not containing the transformed plant cell. Tissue and/or temporal specificity for expression of the modified ETR nucleic acid is controlled by selecting appropriate expression regulation sequences to target the location and/or time of expression of the transformed nucleic acid. The plants are made by transforming at least one plant cell with an appropriate modified ETR nucleic acid, regenerating plants from one or more of the transformed plant cells and selecting at least one plant having the desired phenotype. 31 figs.

  14. Helium Leak Examination Technology on In-Service Condenser in Nuclear Power Plant%核电厂凝汽器在役氦检漏技术

    Institute of Scientific and Technical Information of China (English)

    杨炯; 谢圣华; 钱征宇

    2011-01-01

    凝汽器是核电厂汽轮机组的重要设备,在电厂凝汽式汽轮机组的热力循环中起着冷源的作用,其传热管在运行的过程中可能发生破管,从而引起二回路水质恶化.介绍了针对核电厂常规岛凝汽器破管的氦气泄漏检测方法.通过对在役凝汽器氦气泄漏检测方法的研究,掌握传热管破管的判断方法.现场检测结果表明,开发的检验技术应用效果良好,在保证核电厂安全、经济运行上起到了重要的作用.%Condenser is the very important equipment in the steamship unit of the nuclear power plant and it plays a cooling effect on the heat circulation in the condensable steamship unit of the nuclear power plant. Pipe rupture in the process of operation might occur in its heat transfer canal and it will lead to the deterioration of water quality in the Second Circuit. This article introduced the helium leak examination method against the pipe rupture in the condenser in the steamship unit of the nuclear power plant, grasping the judgment method whether the pipe rupture occurs in heat transfer canal by researching the helium leak examination method on the in-service condenser. The on-site examination results indicated the developed examination technology apply well, it played an important effect on assuring the safety and economic operation of the nuclear power plant.

  15. Transforming classroom practices through teachers' learning of TPACK: The case of in-service teachers at Kibasila Secondary School in Tanzania

    NARCIS (Netherlands)

    Kafyulilo, Ayoub; Fisser, Petra; Voogt, Joke; Resta, P.

    2012-01-01

    This study investigated the extent to which teachers’ learning of Technological Pedagogical Content Knowledge (TPACK) has an impact on their technology use and classroom practices. The study involved 12 in-service teachers and 40 students from Kibasila secondary school in Tanzania. During the study,

  16. From hesitation to appreciation: the transformation of a single, local donation-nurse project into an established organ-donation service.

    Science.gov (United States)

    Gyllström Krekula, Linda; Malenicka, Silvia; Nydahl, Anders; Tibell, Annika

    2015-03-01

    This study evaluates the transition from a local project to promote organ donation to a permanent county-based donation service inspired by the Spanish model. To address the problem of declining donation rates, a project with one donation-specialized nurse (DOSS) was initiated at a single neuro-intensive care unit. This project was later expanded into a permanent on-call service consisting of seven DOSSes, covering a large urban county. During the different periods (before, during project and during permanent service), the DOSS function's effect on donation rates was significant, and the number of eligible donors that became actual donors increased from 37% to 73% and 74%, respectively. The effect on family vetoes was as prominent with a decrease from 34% to 8% and 14%. The staff appreciation of the DOSS function was also evident during the periods; all areas included in the questionnaire (family care, donor care and staff support) have improved greatly owing to the DOSS. The transition from a single, local donation-nurse project, to an on-call service with several DOSSes covering a large urban county was a success considering the donation rates as well as the staff's appreciation. Hence, organizational models from abroad can be adjusted and successfully adopted.

  17. Transforming classroom practices through teachers' learning of TPACK: The case of in-service teachers at Kibasila Secondary School in Tanzania

    NARCIS (Netherlands)

    Kafyulilo, A.; Fisser, P.H.G.; Voogt, Joke; Resta, P.

    2012-01-01

    This study investigated the extent to which teachers’ learning of Technological Pedagogical Content Knowledge (TPACK) has an impact on their technology use and classroom practices. The study involved 12 in-service teachers and 40 students from Kibasila secondary school in Tanzania. During the study,

  18. [Construction of a bivalent plant expression vector carrying VvSUC11 and VvSUC12 genes and its genetic transformation in sugar beet].

    Science.gov (United States)

    Yin, Donglin; Zhu, Jianbo; Wang, Aiying; Xiang, Benchun

    2011-08-01

    We have recombined genes VvSUC11, VvSUC12 from Vitis vinifera L., and root-specific promoters of sweet potato storage protein gene from Ipomoea batatas L. Lam., named as SP1 and SP2. We have constructed a vector pCAMBIA2301-SP1- VvSUC11-SP2-VvSUC12 using pCAMBIA2301 as an original vector. VvSUC11 and VvSUC12 were under the control of root-specific promoters of sweet potato storage protein gene. We transformed the vector into KWS-9103 breeding line of Beta vulgaris L. with Agrobacterium-mediated transformation. We have established the optimal genetic transformation protocol of sugar beet as following: the explants pre-cultured for 4 days were immersed in Agrobacterium suspension of OD(600)=0.5, supplemented with 0.005% Silwet L-77, and followed by a 4-day culture on medium containing cefotaxime, then the buds were selected on medium containing kanamycin and cefotaxime. The percentage of kanamycin-resistant buds was as high as 42%. Results of PCR and RT-PCR proved that the target genes had integrated into sugar beet genome and expressed. It will lay a foundation for further studying their function in Beta vulgaris.

  19. Trade-offs Between Electricity Production from Small Hydropower Plants and Ecosystem Services in Alpine River Basins

    Science.gov (United States)

    Meier, Philipp; Schwemmle, Robin; Viviroli, Daniel

    2015-04-01

    The need for a reduction in greenhouse gas emissions and the decision to phase out nuclear power plants in Switzerland and Germany increases pressure to develop the remaining hydropower potential in Alpine catchments. Since most of the potential for large reservoirs is already exploited, future development focusses on small run-of-the-river hydropower plants (SHP). Being considered a relatively environment-friendly electricity source, investment in SHP is promoted through subsidies. However, SHP can have a significant impact on riverine ecosystems, especially in the Alpine region where residual flow reaches tend to be long. An increase in hydropower exploitation will therefore increase pressure on ecosystems. While a number of studies assessed the potential for hydropower development in the Alps, two main factors were so far not assessed in detail: (i) ecological impacts within a whole river network, and (ii) economic conditions under which electricity is sold. We present a framework that establishes trade-offs between multiple objectives regarding environmental impacts, electricity production and economic evaluation. While it is inevitable that some ecosystems are compromised by hydropower plants, the context of these impacts within a river network should be considered when selecting suitable sites for SHP. From an ecological point of view, the diversity of habitats, and therefore the diversity of species, should be maintained within a river basin. This asks for objectives that go beyond lumped parameters of hydrological alteration, but also consider habitat diversity and the spatial configuration. Energy production in run-of-the-river power plants depends on available discharge, which can have large fluctuations. In a deregulated electricity market with strong price variations, an economic valuation should therefore be based on the expected market value of energy produced. Trade-off curves between different objectives can help decision makers to define policies

  20. Leaching of plant protection products and their transformation products : proposals for improving the assessment of leaching to groundwater in the Netherlands : version 2

    NARCIS (Netherlands)

    Boesten, J.J.T.I.; Linden, van der A.M.A.; Beltman, W.H.J.; Pol, J.W.

    2015-01-01

    Assessment of leaching of plant protection products to groundwater is an important aspect of the environmental risk assessment of these substances. Analysis of available Dutch groundwater monitoring data for these substances triggered a critical review of the current Dutch leaching assessment. As a

  1. Identification of microplastic in effluents of waste water treatment plants using focal plane array-based micro-Fourier-transform infrared imaging

    NARCIS (Netherlands)

    Mintenig, S.M.; Int-Veen, I.; Löder, M.G.J.; Primpke, S.; Gerdts, G.

    2017-01-01

    Abstract The global presence of microplastic (MP) in aquatic ecosystems has been shown by various studies. However, neither MP concentrations nor their sources or sinks are completely known. Waste water treatment plants (WWTPs) are considered as significant point sources discharging MP to the enviro

  2. Resistance to Cucumber mosaic virus in Gladiolus plants transformed with either a defective replicase of coat protein subgroup II gene from Cucumber mosaic virus

    Science.gov (United States)

    Transgenic Gladiolus plants that contain either Cucumber mosaic virus (CMV) subgroup I coat protein, CMV subgroup II coat protein, CMV replicase, a combination of the CMV subgroups I and II coat proteins, or a combination of the CMV subgroup II coat protein and replicase genes were developed. These...

  3. Identification of microplastic in effluents of waste water treatment plants using focal plane array-based micro-Fourier-transform infrared imaging

    NARCIS (Netherlands)

    Mintenig, S.M.; Int-Veen, I.; Löder, M.G.J.; Primpke, S.; Gerdts, G.

    2017-01-01

    Abstract The global presence of microplastic (MP) in aquatic ecosystems has been shown by various studies. However, neither MP concentrations nor their sources or sinks are completely known. Waste water treatment plants (WWTPs) are considered as significant point sources discharging MP to the enviro

  4. Mechanisms of Selenium Uptake, Translocation and Chemical Speciation Transformation in Plants%植物对硒的吸收转运和形态转化机制

    Institute of Scientific and Technical Information of China (English)

    王晓芳; 陈思杨; 罗章; 黄青青; 乔玉辉; 孙宏杰; 李花粉

    2014-01-01

    阐述了植物对不同形态硒的吸收、转运和形态转化机制。植物主要吸收水溶性硒,包括部分有机硒、硒酸盐和亚硒酸盐。多数研究表明植物对硒酸盐的主动吸收是通过高亲和力的硫酸盐转运子完成,最近的研究表明磷酸盐可以调节亚硒酸盐的吸收,磷酸盐转运子在亚硒酸盐的主动吸收过程中有重要作用;植物吸收的硒酸盐很快从根部转移到地上部,在叶片中被还原成亚硒酸盐,进而转化为有机硒化物进入其他组织;而亚硒酸盐及其代谢产物主要积累在根部,极少转移到地上部。进入植物体中的硒转化为含硒氨基酸和硒蛋白参与植物的代谢。%In this review, we summarized the uptake, translocation and metabolism mechanisms of selenium(Se)in plants. Water-soluble Se, including selenate, selenite and some organic selenium, was the predominant form taken up by plants. The active uptake of selenate in plant could be mediated by high affinity sulfate transporters. The mechanism of selenite uptake was not well understood, however, recent studies suggested that selenite uptake was probably mediated by Pi transporters. The translocation of Se from root to shoot was dependent on the form of Se supplied. The selenate taken up by plants was readily translocated to the shoots and reduced to selenite in leaves, then convert-ed to organic Se compound. By contrast, most of the selenite taken up by plants in the roots and only a small fraction was translocated to the shoots. Se could be assimilated to seleno amino acids or selenoprotein after plant uptake, and then involved in the metabolism of plants.

  5. Service life prediction. Development of models for predicting the service life of power plant components subject to thermomechanical creep fatigue; Lebensdauervorhersage. Entwicklung von Modellen zur Lebensdauervorhersage von Kraftwerksbauteilen unter thermisch-mechanischer Kriechermuedungsbeanspruchung. Abschlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Cui, L.; Scholz, A. [Technische Univ. Darmstadt (Germany). Institut fuer Werkstoffkunde; Hartrott, P. von; Schlesinger, M. [Fraunhofer-Institut fuer Werkstoffmechanik (IWM), Freiburg im Breisgau (Germany)

    2009-07-01

    Extensive use is made of massive components of heat resistant and highly heat resistant materials in installations of the power and heating industry. These components are exposed to varying thermomechanical stress as a result of ramping-up and down processes. In this research project two computer-assisted methods of predicting service life until crack initiation were extended to include cases of thermomechanical multi-axis stress conducive to creep fatigue and of superposition of high-cycle stress on power plant components. Investigations were limited to rotor steel of type X12CrMoWVNbN10-1-1. Complex thermomechanical multi-axis experiments were performed on round, notched and cruciform test specimens of close-to-life dimensions in order to demonstrate by experiment the validity of these models. The results of these calculations showed an acceptable degree of agreement between experiment and simulation for both models. Calculations on earlier TMF experiments performed at IfW on hollow specimens of 1%CrMoNiV showed good predictability for both the SARA and the ThoMat programme. Calculations on experiments performed at MPA Stuttgart on model bodies consisting of the same 1%CrMoNiV showed a predictability of acceptable variability considering the complexity of the stresses involved. A further outcome of this project is that the use of SARA appears universally suitable for the construction of new plants and in the service area, while the use of ThoMat appears suited for detail optimisation in the development process.

  6. 城市功能及其服务转型趋势分析%The analysis on urban function and its service transformation trend

    Institute of Scientific and Technical Information of China (English)

    陈玉英

    2009-01-01

    21世纪是城市的世纪,城市发展对中国经济发展将起决定性作用.而城市功能是城市发展的重要课题,其发展转型决定着城市的盛衰存亡.本文以城市功能的辞意剖析为切入点,透视城市发展的历史天际线,概括历史中的城市功能类型,探寻其变化、演进特征属性,并进一步提出创造和使用城市的人调控城市功能转型的必然与可能路径.%In 21 centuries, City will have very important function in country economy. City development to China economy development will have decisive function. The city function will be a city development of importance topic, it development transformation decision the rise and fail alive or dead of city. The paper analysis of the city function as a correspond point. Seeing through city development of history horizon line, this paper generalize urban function within history type, and explore its variety,evolve characteristic attribute, and further put forward creation and usage city of the person adjust to control city function transformation of inevitable and possibility path.

  7. Strategic Transformation of Special Libraries

    Institute of Scientific and Technical Information of China (English)

    刘细文

    2015-01-01

    Under the shocks of digitalization and networking,National Science Library of the Chinese Academy of Sciences(NSLC)has executed the transformation strategy for knowledge-based services,and fully pushed forward the literature information services of NSLC.Supervised by the Chinese Academy of Sciences(CAS),NSLC has established the Literature and Information Branch of Chinese Alliance of

  8. Transforming maternal and newborn health social norms and practices to increase utilization of health services in rural Bangladesh: a qualitative review.

    Science.gov (United States)

    Taleb, Fahmida; Perkins, Janet; Ali, Nabeel Ashraf; Capello, Cecilia; Ali, Muzahid; Santarelli, Carlo; Hoque, Dewan Md Emdadul

    2015-03-29

    Since 2008, Participatory Action for Rural Development Innovation (PARI) Development Trust, with the support of Enfants du Monde, has been implementing a maternal and newborn health (MNH) program based on the World Health Organization's (WHO) framework for Working with Individuals, Families and Communities (IFC) to improve MNH in Netrokona district, Bangladesh. This program aims to empower women and families and increase utilization of quality health services, thereby helping women realize their rights related to maternal health. Birth preparedness and complication readiness and working with traditional birth attendants (TBAs) to exercise a new role in MNH and have formed key interventions of this program. The purpose of this study was to explore how the program has contributed to changing social norms and practices surrounding MNH at midpoint. This study relied primarily on qualitative data collection. Two focus group discussions (FGDs) were conducted with women who were pregnant or had recently given birth and one FGD with each of the following groups: husbands, family members, TBAs, and health workers. In-depth interviews were conducted with women who were pregnant or had recently given birth, family members of these women, health care providers, TBAs and community health workers in selected intervention areas. Since implementation of interventions informants report an increase in planning for birth and complications and a shift in preference toward skilled care at birth. However, women still prefer to receive services at home. TBAs report encouraging women to access skilled care for both routine and emergency services. While community members' understanding of rights related to maternal health remains limited, they report increased women's participation in household decision- making processes, an important indicator of the realization of rights. Results suggest that community-level interventions aiming to affect change in social norms and practices surrounding

  9. Aging and service wear of spring-loaded pressure relief valves used in safety-related systems at nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Staunton, R.H.; Cox, D.F. [Oak Ridge National Lab., TN (United States)

    1995-03-01

    Spring-loaded pressure relief valves (PRVS) are used in some safety-related applications at nuclear power plants. In general, they are used in systems where, during accidents, pressures may rise to levels where pressure safety relief is required for protection of personnel, system piping, and components. This report documents a study of PRV aging and considers the severity and causes of service wear and how it is discovered and corrected in various systems, valve sizes, etc. Provided in this report are results of the examination of the recorded failures and identification of trends and relationships/correlations in the failures when all failure-related parameters are considered. Components that comprise a typical PRV, how those components fail, when they fail, and the current testing frequencies and methods are also presented in detail.

  10. Transformation of government function of health management under the perspective of new public service theory%新公共服务理论视角下的政府卫生管理职能转变

    Institute of Scientific and Technical Information of China (English)

    刘静; 李之; 王宏志

    2008-01-01

    分析了新公共服务理论视角下的政府卫生管理职能定位,揭示了现阶段我国政府卫生管理在医疗卫生服务体系、医疗机构运行机制、医疗卫生管理体制、医疗卫生服务市场监管体系4个方面存在的职能不到位、错位、越位及缺位现象,提出了政府卫生管理职能转变的6条途径.%The paper analyzes the orientation of health management function of government under the perspective of new public service theory, tries to reveal the unimplementation, dislocation, offside and absence of government function at four aspects including health service system, health organization operating mechanism, health management system and health service supervision system, and puts forward six approaches for transformation of government function of health management.

  11. 电信运营商的IDC业务转型浅谈%Discussion on Transformation of Telecom Operators’ IDC Services

    Institute of Scientific and Technical Information of China (English)

    甘志辉; 陈博

    2015-01-01

    随着“宽带中国”、“互联网+”战略上升至国家层面,电信运营商经营的IDC业务面临着重要的发展机遇,同时也面临着前所未有的竞争和困境。应用互联网思维,重点从管理体制、资源布局、业务流程、市场营销等方面提出了一些转型的思考。%With Broadband China and Internet+ strategies enhanced to national level, the IDC business is facing important developmental opportunities and unprecedented competition and dilemma. Combined with Internet thinking, some transformation ideas were put forward from aspects of management system, resource layout, business processes and marketing.

  12. Visualizing Transformation

    DEFF Research Database (Denmark)

    Pedersen, Pia

    2012-01-01

    Transformation, defined as the step of extracting, arranging and simplifying data into visual form (M. Neurath, 1974), was developed in connection with ISOTYPE (International System Of TYpographic Picture Education) and might well be the most important legacy of Isotype to the field of graphic...... design. Recently transformation has attracted renewed interest because of the book ‘The Transformer’ written by Robin Kinross and Marie Neurath. My on-going research project, summarized in this paper, identifies and depicts the essential principles of data visualization underlying the process...... of transformation with reference to Marie Neurath’s sketches on the Bilston Project. The material has been collected at the Otto and Marie Neurath Collection housed at the University of Reading, UK. By using data visualization as a research method to look directly into the process of transformation the project...

  13. Transformational leadership.

    Science.gov (United States)

    Luzinski, Craig

    2011-12-01

    This month, the director of the Magnet Recognition Program® takes an in-depth look at the Magnet® model component transformational leadership. The author examines the expectations for Magnet organizations around this component. What are the qualities that make a nursing leader truly transformational, and what is the best approach to successfully lead a healthcare organization through today's volatile healthcare environment?

  14. Landskabets transformation

    DEFF Research Database (Denmark)

    Munck Petersen, Rikke

    2005-01-01

    Seminaroplæg fra forskere. Faglige seminarer på KA, forår 2005. Belyser transformation af det danske landskab fysisk som holdningsmæssigt, samt hvordan phd-arbejdets egen proces håndterer den.......Seminaroplæg fra forskere. Faglige seminarer på KA, forår 2005. Belyser transformation af det danske landskab fysisk som holdningsmæssigt, samt hvordan phd-arbejdets egen proces håndterer den....

  15. Estimates of the impacts of invasive alien plants on water flows in ...

    African Journals Online (AJOL)

    species and their invasion ecology also differs (Van Wilgen et al., ... as transformed (e.g. cultivated, urban) in the national land cover ... spatial data sets supplied with the vegetation map (Mucina and Rutherford, 2006), and the land types from the database ...... impact of invasive alien plants on ecosystem services in South.

  16. Waste sulphuric acid regeneration plants and on-site services%废硫酸再生装置与现场服务

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Waste sulphuric acid regeneration has matured to provide a robust and reliable way for recycling the waste sulphuric acid using a variety of sources. Design features of MECS waste sulphuric acid regeneration plants and on-site service for sulphuric users provided by Rhodia are described. Rhodia's service includes a patented storage tank cleaning technology, which can not only clean out and recover acid sludge from storage tanks, but also avoid confined space entry.%废硫酸再生技术已经成熟,为回收利用各种来源的废硫酸提供了一条稳健、可靠的途径。主要阐述了MECS废酸再生装置的设计特点和罗地亚为硫酸用户提供的现场服务。罗地亚的现场服务包括一种专利储罐清洗技术,该技术不但可清除并回收罐底酸泥,而且可避免受限空间的进入。

  17. Renovation and transformation of radial service gate for flood relief tunnel of Shitouhe Reservoir%石头河水库泄洪洞弧形工作闸门(深孔)的更新改造

    Institute of Scientific and Technical Information of China (English)

    罗宝财

    2015-01-01

    介绍了石头河水库泄洪洞弧形工作闸门存在的锈蚀、焊接质量差、启闭设备动力不足等问题,论述了除险加固中,金属结构及机电设备更新改造工艺及方法.%An introduction was made on the problems of the radial service gate at the flood relief tunnel of Shitouhe Reservoir such as rust erosion, poor welding quality and insufficient power for hoisting equipment, based on which the author presented the technique and method adopted for renovation and transformation of metal works, mechanical and electrical equipment.

  18. Novel Control Strategy for Multiple Run-of-the-River Hydro Power Plants to Provide Grid Ancillary Services

    Energy Technology Data Exchange (ETDEWEB)

    Mohanpurkar, Manish; Luo, Yusheng; Hovsapian, Rob; Muljadi, Eduard; Gevorgian, Vahan; Koritarov, Vladimir

    2017-07-12

    Hydropower plant (HPP) generation comprises a considerable portion of bulk electricity generation and is delivered with a low-carbon footprint. In fact, HPP electricity generation provides the largest share from renewable energy resources, which include wind and solar. Increasing penetration levels of wind and solar lead to a lower inertia on the electric grid, which poses stability challenges. In recent years, breakthroughs in energy storage technologies have demonstrated the economic and technical feasibility of extensive deployments of renewable energy resources on electric grids. If integrated with scalable, multi-time-step energy storage so that the total output can be controlled, multiple run-of-the-river (ROR) HPPs can be deployed. Although the size of a single energy storage system is much smaller than that of a typical reservoir, the ratings of storages and multiple ROR HPPs approximately equal the rating of a large, conventional HPP. This paper proposes cohesively managing multiple sets of energy storage systems distributed in different locations. This paper also describes the challenges associated with ROR HPP system architecture and operation.

  19. Novel Control Strategy for Multiple Run-of-the-River Hydro Power Plants to Provide Grid Ancillary Services

    Energy Technology Data Exchange (ETDEWEB)

    Mohanpurkar, Manish; Luo, Yusheng; Hovsapian, Rob; Muljadi, Eduard; Gevorgian, Vahan; Koritarov, Vladimir

    2017-05-01

    Electricity generated by Hydropower Plants (HPPs) contributes a considerable portion of bulk electricity generation and delivers it with a low carbon footprint. In fact, HPP electricity generation provides the largest share from renewable energy resources, which includes solar and wind energy. The increasing penetration of wind and solar penetration leads to a lowered inertia in the grid and hence poses stability challenges. In recent years, breakthrough in energy storage technologies have demonstrated the economic and technical feasibility of extensive deployments in power grids. Multiple ROR HPPs if integrated with scalable, multi time-step energy storage so that the total output can be controlled. Although, the size of a single energy storage is far smaller than that of a typical reservoir, cohesively managing multiple sets of energy storage distributed in different locations is proposed. The ratings of storages and multiple ROR HPPs approximately equals the rating of a large, conventional HPP. The challenges associated with the system architecture and operation are described. Energy storage technologies such as supercapacitors, flywheels, batteries etc. can function as a dispatchable synthetic reservoir with a scalable size of energy storage will be integrated. Supercapacitors, flywheels, and battery are chosen to provide fast, medium, and slow responses to support grid requirements. Various dynamic and transient power grid conditions are simulated and performances of integrated ROR HPPs with energy storage is provided. The end goal of this research is to investigate the inertial equivalence of a large, conventional HPP with a unique set of multiple ROR HPPs and optimally rated energy storage systems.

  20. A Case Study of Nearshore Wave Transformation Processes along the Coast of Mexico near the Laguna Verde Nuclear Power Plant Using a Fast Simulation Method

    Directory of Open Access Journals (Sweden)

    Israel-Enrique Herrera-Díaz

    2010-01-01

    numerical method was developed using a split proposed version of the mild-slope equation and solved by an implicit method in a finite volume grid; this technique easily allows model the wave effects caused by the breakwater building in coastal waters, where industrial and other economic activities take place. Controlled case studies have been made and the results match very well with the reference solution. The capability and utility of the model for real coastal areas are illustrated by application to the breakwater of the Laguna Verde Nuclear Power Plant (LVNPP.