WorldWideScience

Sample records for plant transformation advances

  1. PLANT TRANSFORMATION: ADVANCES AND PERSPECTIVES

    Directory of Open Access Journals (Sweden)

    Alves Adriana Cristina

    1999-01-01

    Full Text Available Genetic transformation is a powerful tool for plant breeding and genetical, physiological or biochemical research, consequently it is an extremely dynamic field. Transgenic plants are commonly used to complete or substitute mutants in basic research, helping the studies of complex biological situations such as pathogenesis process, genome organization, light reception and signal transduction. In this review, recent approaches for foreign gene introduction (e.g. Agrobiolistics, whole tissue electroporation, in planta Agrobacterium transformation, screening (reporter gene possibilities and performance and transformant selection (ipt selective marker are discussed. Transgene expression and mechanisms underlying (transgene inactivation are presented. Practical applications of genetically modified plants, field tests and commercial transgenic crops worldwide and in Brazil are listed, as well as the main traits and species modified. Potential uses of transgenic plants for animal compound production, biological remediation and synthetic polymer assembly are also shown.

  2. PLANT TISSUE CULTURE IN BIOTECHNOLOGY: RECENT ADVANCES IN TRANSFORMATION THROUGH SOMATIC EMBRYOGENESIS

    Directory of Open Access Journals (Sweden)

    V. A. Sidorov

    2012-08-01

    Full Text Available Plant genetic transformation has become an important biotechnology tool for the improvement of many crops. A solid foundation for the fast development and implementation of biotechnology in agriculture was provided by achievements in plant tissue culture. On the 30th anniversary of plant transformation, I report the advancements, recent challenges and shifts in methodology of transformation. The main focus of this paper will be on conventional and novel approaches for genetic improvements of soybean, cotton and corn. I will also highlight results on the transformation of these crops that have considerably been improved by modern biotechnology.

  3. Advancing Crop Transformation in the Era of Genome Editing

    Science.gov (United States)

    Plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. Unfortunately, for most crops, transformation and regeneration remain arduous even after more than thirty years of technological advances. Genome editing provides new opportunities to...

  4. Technological advances transforming rheumatology

    Science.gov (United States)

    Robinson, William H.; Mao, Rong

    2016-01-01

    Technological advances over the past decade have revolutionized many areas of rheumatology, ranging from diagnosis, prognosis and therapeutic development to the mechanistic understanding of rheumatic diseases. This overview highlights key technological innovations and discusses the major impact that these developments are having on research and clinical practice. PMID:26439404

  5. Micro-shock Wave Assisted Plant Transformation

    Science.gov (United States)

    Gnanadhas, Divya Prakash; Datey, Akshay; Chakravortty, Dipshikha; Gopalan, Jagadeesh

    Genetically modified (GM) crops are developed by transforming the desired DNA to plant. There are various methods employed to achieve the required transformation in plants. Agrobacterium mediated transformation and Biolistics or particle bombardment method are the most commonly used methods.

  6. Advancing Crop Transformation in the Era of Genome Editing.

    Science.gov (United States)

    Altpeter, Fredy; Springer, Nathan M; Bartley, Laura E; Blechl, Ann E; Brutnell, Thomas P; Citovsky, Vitaly; Conrad, Liza J; Gelvin, Stanton B; Jackson, David P; Kausch, Albert P; Lemaux, Peggy G; Medford, June I; Orozco-Cárdenas, Martha L; Tricoli, David M; Van Eck, Joyce; Voytas, Daniel F; Walbot, Virginia; Wang, Kan; Zhang, Zhanyuan J; Stewart, C Neal

    2016-07-01

    Plant transformation has enabled fundamental insights into plant biology and revolutionized commercial agriculture. Unfortunately, for most crops, transformation and regeneration remain arduous even after more than 30 years of technological advances. Genome editing provides novel opportunities to enhance crop productivity but relies on genetic transformation and plant regeneration, which are bottlenecks in the process. Here, we review the state of plant transformation and point to innovations needed to enable genome editing in crops. Plant tissue culture methods need optimization and simplification for efficiency and minimization of time in culture. Currently, specialized facilities exist for crop transformation. Single-cell and robotic techniques should be developed for high-throughput genomic screens. Plant genes involved in developmental reprogramming, wound response, and/or homologous recombination should be used to boost the recovery of transformed plants. Engineering universal Agrobacterium tumefaciens strains and recruiting other microbes, such as Ensifer or Rhizobium, could facilitate delivery of DNA and proteins into plant cells. Synthetic biology should be employed for de novo design of transformation systems. Genome editing is a potential game-changer in crop genetics when plant transformation systems are optimized.

  7. Advanced genetic tools for plant biotechnology

    Energy Technology Data Exchange (ETDEWEB)

    Liu, WS; Yuan, JS; Stewart, CN

    2013-10-09

    Basic research has provided a much better understanding of the genetic networks and regulatory hierarchies in plants. To meet the challenges of agriculture, we must be able to rapidly translate this knowledge into generating improved plants. Therefore, in this Review, we discuss advanced tools that are currently available for use in plant biotechnology to produce new products in plants and to generate plants with new functions. These tools include synthetic promoters, 'tunable' transcription factors, genome-editing tools and site-specific recombinases. We also review some tools with the potential to enable crop improvement, such as methods for the assembly and synthesis of large DNA molecules, plant transformation with linked multigenes and plant artificial chromosomes. These genetic technologies should be integrated to realize their potential for applications to pressing agricultural and environmental problems.

  8. Advanced genetic tools for plant biotechnology.

    Science.gov (United States)

    Liu, Wusheng; Yuan, Joshua S; Stewart, C Neal

    2013-11-01

    Basic research has provided a much better understanding of the genetic networks and regulatory hierarchies in plants. To meet the challenges of agriculture, we must be able to rapidly translate this knowledge into generating improved plants. Therefore, in this Review, we discuss advanced tools that are currently available for use in plant biotechnology to produce new products in plants and to generate plants with new functions. These tools include synthetic promoters, 'tunable' transcription factors, genome-editing tools and site-specific recombinases. We also review some tools with the potential to enable crop improvement, such as methods for the assembly and synthesis of large DNA molecules, plant transformation with linked multigenes and plant artificial chromosomes. These genetic technologies should be integrated to realize their potential for applications to pressing agricultural and environmental problems.

  9. Advanced stellarator power plants

    Energy Technology Data Exchange (ETDEWEB)

    Miller, R.L.

    1994-07-01

    The stellarator is a class of helical/toroidal magnetic fusion devices. Recent international progress in stellarator power plant conceptual design is reviewed and comparisons in the areas of physics, engineering, and economics are made with recent tokamak design studies.

  10. Advanced stellarator power plants

    Energy Technology Data Exchange (ETDEWEB)

    Miller, R.L.

    1994-07-01

    The stellarator is a class of helical/toroidal magnetic fusion devices. Recent international progress in stellarator power plant conceptual design is reviewed and comparisons in the areas of physics, engineering, and economics are made with recent tokamak design studies.

  11. Physical methods for genetic plant transformation

    Science.gov (United States)

    Rivera, Ana Leonor; Gómez-Lim, Miguel; Fernández, Francisco; Loske, Achim M.

    2012-09-01

    Production of transgenic plants is a routine process for many crop species. Transgenes are introduced into plants to confer novel traits such as improved nutritional qualities, tolerance to pollutants, resistance to pathogens and for studies of plant metabolism. Nowadays, it is possible to insert genes from plants evolutionary distant from the host plant, as well as from fungi, viruses, bacteria and even animals. Genetic transformation requires penetration of the transgene through the plant cell wall, facilitated by biological or physical methods. The objective of this article is to review the state of the art of the physical methods used for genetic plant transformation and to describe the basic physics behind them.

  12. Transformation of medicinal plants using Agrobacterium tumefaciens.

    Science.gov (United States)

    Bandurska, Katarzyna; Berdowska, Agnieszka; Król, Małgorzata

    2016-12-20

    For many years attempts are made to develop efficient methods for transformation of medicinal plants via Agrobacterium tumefaciens. It is a soil bacteria which possess a natural ability to infect plants in places of injures which results in arise of cancerous growths (crown gall). This is possible thanks a transfer of fragment of Ti plasmid into plant cells and stable integration with a plant genome. Efficiency of medicinal plant transformation depends on many factors for example: Agrobacterium strain, methods and procedures of transformation as well as on plant species, type and age of the explants and regeneration conditions. The main goal of plant transformation is to increase the amount of naturally occurring bioactive compounds and the production of biopharmaceuticals. Genetic plant transformation via bacteria of the genus Agrobacterium is a complex process which requires detailed analysis of incorporated transgene expression and occurs only in the case when the plant cell acquires the ability to regenerate. In many cases, the regeneration efficiency observed in medicinal plants are inefficient after applied transformation procedures. To date there have been attempts of genetic transformation by using A. tumefaciens of medicinal plants belonging to the families: Apocynaceae, Araceae, Araliaceae, Asphodelaceae, Asteraceae, Begoniaceae, Crassulaceae, Fabaceae, Lamiaceae, Linaceae, Papaveraceae, Plantaginaceae, Scrophulariaceae and Solanaceae.

  13. Advanced light water reactor plant

    Energy Technology Data Exchange (ETDEWEB)

    Giedraityte, Zivile [Helsinki University of Technology, Otaranta 8D-84, 02150 Espoo (Finland)

    2008-07-01

    For nuclear power to be competitive with the other methods of electrical power generation the economic performance should be significantly improved by increasing the time spent on line generating electricity relative to time spent off-line conducting maintenance and refueling. Maintenance includes planned actions (surveillances) and unplanned actions (corrective maintenance) to respond to component degradation or failure. A methodology is described which is used to resolve maintenance related operating cycle length barriers. Advanced light water nuclear power plant is designed with the purpose to maximize online generating time by increasing operating cycle length. (author)

  14. Advances in Agrobacterium-mediated plant transformation with enphasys on soybean Avanços na transformação genética de plantas mediada por Agrobacterium com ênfase em soja

    Directory of Open Access Journals (Sweden)

    Paulo Celso de Mello-Farias

    2008-02-01

    Full Text Available Soybean is one of humanity's major sources of plant protein. It is also very important for animal feed and as industrial raw material. Great advances have recently been achieved in its genetic transformation. This review provides a comprehensive discussion of important factors affecting Agrobacterium-mediated soybean transformation including target tissues, plant tissue health, wounding methods, regeneration systems, selectable markers and reporter genes.A soja é uma das maiores fontes de proteína vegetal da humanidade. É também muito importante na alimentação animal e como matéria-prima industrial. Grandes avanços foram recentemente alcançados em sua transformação genética. O objetivo desta revisão é fornecer uma discussão acerca de importantes fatores que influenciam a transformação de soja mediada por Agrobaterium, incluindo tecidos alvo, condições do tecido vegetal, métodos de injúria, sistemas de regeneração, marcadores de seleção e genes repórter.

  15. Some Recent Advances in Plant Physiology

    Science.gov (United States)

    Stafford, G. A.

    1972-01-01

    A popular review of plant physiological research, emphasizing those apsects of plant metabolism where there has been a recent shift in emphasis that is not yet reflected in secondary school advanced texts. (AL)

  16. Recent advances in the genetic transformation of coffee.

    Science.gov (United States)

    Mishra, M K; Slater, A

    2012-01-01

    Coffee is one of the most important plantation crops, grown in about 80 countries across the world. The genus Coffea comprises approximately 100 species of which only two species, that is, Coffea arabica (commonly known as arabica coffee) and Coffea canephora (known as robusta coffee), are commercially cultivated. Genetic improvement of coffee through traditional breeding is slow due to the perennial nature of the plant. Genetic transformation has tremendous potential in developing improved coffee varieties with desired agronomic traits, which are otherwise difficult to achieve through traditional breeding. During the last twenty years, significant progress has been made in coffee biotechnology, particularly in the area of transgenic technology. This paper provides a detailed account of the advances made in the genetic transformation of coffee and their potential applications.

  17. Recent Advances in the Genetic Transformation of Coffee

    Directory of Open Access Journals (Sweden)

    M. K. Mishra

    2012-01-01

    Full Text Available Coffee is one of the most important plantation crops, grown in about 80 countries across the world. The genus Coffea comprises approximately 100 species of which only two species, that is, Coffea arabica (commonly known as arabica coffee and Coffea canephora (known as robusta coffee, are commercially cultivated. Genetic improvement of coffee through traditional breeding is slow due to the perennial nature of the plant. Genetic transformation has tremendous potential in developing improved coffee varieties with desired agronomic traits, which are otherwise difficult to achieve through traditional breeding. During the last twenty years, significant progress has been made in coffee biotechnology, particularly in the area of transgenic technology. This paper provides a detailed account of the advances made in the genetic transformation of coffee and their potential applications.

  18. Transformation of plant young proembryos by electroporation

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    It is first reported that plant young proembryos expressed exogenous reporter genes by electroporation. Young proembryos with 8-32 cells and globular proembryos with 250-400 cells could be isolated by enzymatic maceration combined with microdissection. After electroporation with GUS or GFP genes, the proembryos were cultured for 1-2 d in KM8p medium. At the field strength of electroporation 500-1 500 V/cm, blue reaction of GUS or green fluorescence of GFP could be observed in the proembryos. The highest transient expression frequency of young proembryos (2.2%) was obtained at the field strength of 750 V/cm, whereas the highest frequency of globular proembryos (5.9%) was obtained at the field strength of 1 250 V/cm. Taking the proportion of transformed cells in the whole cells of proembryos as efficient transformation frequency, the efficient transformation frequency of the young proembryos was 7 times that of the globular proembryos.

  19. New advances in virtual plant research

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Virtual plants are computer simulations of the growth, development and deployment of plants in a three-dimensional space. Over the past 20 years, significant pro-gress has been made in virtual plant modeling corresponding to the rapid advances in information technology. Virtual plant research has broad applications in agronomy, forestry, ecology, and remote sensing areas. In this review, we attempt to introduce the significance, modeling methodology, and main advances in virtual plant research and applications. The challenges associated with virtual plant modeling in agronomy applications, including the interaction mechanism between plant and environment and root system modeling, are also discussed. Insights into applications of virtual plants in agronomy are given in the areas of performing virtual experiments to accurately quantify the utilization of soil water and nutrients, to design crop ideotype on computers, and to improve crop planting.

  20. Advanced Coordinating Control System for Power Plant

    Institute of Scientific and Technical Information of China (English)

    WU Peng; WEI Shuangying

    2006-01-01

    The coordinating control system is popular used in power plant. This paper describes the advanced coordinating control by control methods and optimal operation, introduces their principals and features by using the examples of power plant operation. It is wealthy for automation application in optimal power plant operation.

  1. Recent advances in plant-herbivore interactions

    Science.gov (United States)

    Burkepile, Deron E.; Parker, John D.

    2017-01-01

    Plant-herbivore interactions shape community dynamics across marine, freshwater, and terrestrial habitats. From amphipods to elephants and from algae to trees, plant-herbivore relationships are the crucial link generating animal biomass (and human societies) from mere sunlight. These interactions are, thus, pivotal to understanding the ecology and evolution of virtually any ecosystem. Here, we briefly highlight recent advances in four areas of plant-herbivore interactions: (1) plant defense theory, (2) herbivore diversity and ecosystem function, (3) predation risk aversion and herbivory, and (4) how a changing climate impacts plant-herbivore interactions. Recent advances in plant defense theory, for example, highlight how plant life history and defense traits affect and are affected by multiple drivers, including enemy pressure, resource availability, and the local plant neighborhood, resulting in trait-mediated feedback loops linking trophic interactions with ecosystem nutrient dynamics. Similarly, although the positive effect of consumer diversity on ecosystem function has long been recognized, recent advances using DNA barcoding to elucidate diet, and Global Positioning System/remote sensing to determine habitat selection and impact, have shown that herbivore communities are probably even more functionally diverse than currently realized. Moreover, although most diversity-function studies continue to emphasize plant diversity, herbivore diversity may have even stronger impacts on ecosystem multifunctionality. Recent studies also highlight the role of risk in plant-herbivore interactions, and risk-driven trophic cascades have emerged as landscape-scale patterns in a variety of ecosystems. Perhaps not surprisingly, many plant-herbivore interactions are currently being altered by climate change, which affects plant growth rates and resource allocation, expression of chemical defenses, plant phenology, and herbivore metabolism and behavior. Finally, we conclude by

  2. Atmospheric transformation of plant volatiles disrupts host plant finding

    Science.gov (United States)

    Li, Tao; Blande, James D.; Holopainen, Jarmo K.

    2016-09-01

    Plant-emitted volatile organic compounds (VOCs) play important roles in plant-insect interactions. Atmospheric pollutants such as ozone (O3) can react with VOCs and affect the dynamics and fidelity of these interactions. However, the effects of atmospheric degradation of plant VOCs on plant-insect interactions remains understudied. We used a system comprising Brassica oleracea subsp. capitata (cabbage) and the specialist herbivore Plutella xylostella to test whether O3-triggered VOC degradation disturbs larval host orientation, and to investigate the underlying mechanisms. Larvae oriented towards both constitutive and larva-induced cabbage VOC blends, the latter being the more attractive. Such behaviour was, however, dramatically reduced in O3-polluted environments. Mechanistically, O3 rapidly degraded VOCs with the magnitude of degradation increasing with O3 levels. Furthermore, we used Teflon filters to collect VOCs and their reaction products, which were used as odour sources in behavioural tests. Larvae avoided filters exposed to O3-transformed VOCs and spent less time searching on them compared to filters exposed to original VOCs, which suggests that some degradation products may have repellent properties. Our study clearly demonstrates that oxidizing pollutants in the atmosphere can interfere with insect host location, and highlights the need to address their broader impacts when evaluating the ecological significance of VOC-mediated interactions.

  3. Plant regeneration and genetic transformation in Jatropha

    KAUST Repository

    Sujatha, M.

    2012-07-01

    Jatropha curcas, a non-edible oil bearing species with multiple uses, and considerable economic potential is emerging as a potential biofuel plant. The limited knowledge of this species, low and inconsistent yields, the narrow genetic variability, and vulnerability to insects and diseases are major constraints in successful cultivation of Jatropha as a biofuel crop. Hence, genetic improvement of Jatropha is essential by conventional and modern biotechnological tools to use as a viable alternative source of bio-diesel. Realising its potential as a bio-energy crop, in vitro regeneration methods have been established to meet the demand of large scale supply of superior clones, and also as a prelude for genetic improvement of the species through transgenic approaches. In this chapter, an overview of in vitro tissue culture and genetic transformation of Jatropha is discussed. © 2013 Springer Science+Business Media Dordrecht. All rights are reserved.

  4. Recent advances of metabolomics in plant biotechnology.

    Science.gov (United States)

    Okazaki, Yozo; Saito, Kazuki

    2012-01-01

    Biotechnology, including genetic modification, is a very important approach to regulate the production of particular metabolites in plants to improve their adaptation to environmental stress, to improve food quality, and to increase crop yield. Unfortunately, these approaches do not necessarily lead to the expected results due to the highly complex mechanisms underlying metabolic regulation in plants. In this context, metabolomics plays a key role in plant molecular biotechnology, where plant cells are modified by the expression of engineered genes, because we can obtain information on the metabolic status of cells via a snapshot of their metabolome. Although metabolome analysis could be used to evaluate the effect of foreign genes and understand the metabolic state of cells, there is no single analytical method for metabolomics because of the wide range of chemicals synthesized in plants. Here, we describe the basic analytical advancements in plant metabolomics and bioinformatics and the application of metabolomics to the biological study of plants.

  5. Plant maintenance and advanced reactors, 2006

    Energy Technology Data Exchange (ETDEWEB)

    Agnihotri, Newal (ed.)

    2006-09-15

    The focus of the September-October issue is on plant maintenance and advanced reactors. Major articles/reports in this issue include: Advanced plants to meet rising expectations, by John Cleveland, International Atomic Energy Agency, Vienna; A flexible and economic small reactor, by Mario D. Carelli and Bojan Petrovic, Westinghouse Electric Company; A simple and passively safe reactor, by Yury N. Kuznetsov, Research and Development Institute of Power Engineering (NIKIET), Russia; Gas-cooled reactors, by Jeffrey S. Merrifield, U.S. Nuclear Regulatory Commission; ISI project managment in the PRC, by Chen Chanbing, RINPO, China; and, Fort Calhoun refurbishment, by Sudesh Cambhir, Omaha Public Power District.

  6. Advanced coal-fired power plants

    Energy Technology Data Exchange (ETDEWEB)

    Hebel, G.; Weirich, P.H.

    1988-02-01

    Reconstruction of coal-fired power plants under the aspects of higher economic efficiency and lower emissions has become more interesting as the petroleum and natural gas reserves have become shorter. A number of advanced concepts have been presented in the last few years and tested in experimental facilities, pilot plants and demonstration plants. If construction is envisaged within the next five years, better steam processes and coal gas turbines should be employed. Supercharged steam generators, which will bring about further improvements, will be available by the mid-Nineties.

  7. AGROBACTERIUM-MEDIATED TRANSFORMATION OF COMPOSITAE PLANTS. I. CONSTRUCTION OF TRANSGENIC PLANTS AND «HAIRY» ROOTS WITH NEW PROPERTIES

    Directory of Open Access Journals (Sweden)

    N. A.Matvieieva

    2013-02-01

    Full Text Available The review explores some of the recent advances and the author's own researchs concerning biotechnological approaches for Agrobacterium tumefaciens- and A. rhizogenes-mediated transformation of Compositae family plants. This paper reviews the results of genetic transformation of Compositae plants, including edible (Cichorium intybus, Lactuca sativa, oil (Helianthus annuus, decorative (Gerbera hybrida, medical (Bidens pilosa, Artemisia annua, Artemisia vulgaris, Calendula officinalis, Withania somnifera etc. plant species. Some Compositae genetic engineering areas are considered including creation of plants, resistant to pests, diseases and herbicides, to the effect of abiotic stress factors as well as plants with altered phenotype. The article also presents the data on the development of biotechnology for Compositae plants Cynara cardunculus, Arnica montana, Cichorium intybus, Artemisia annua "hairy" roots construction.

  8. Advanced coal-fired power plant technology

    Energy Technology Data Exchange (ETDEWEB)

    Klauke, F. [Babcock Borsig Power Energy GmbH (Germany)

    2001-07-01

    This paper presents the joint efforts of a large European group of manufacturers, utilities and institutes co-operating in a phased long-term project named 'Advanced 700{degree}C PF Power Plant'. Net efficiences of more than 50% will be reached through development of a super critical steam cycle operating at maximum steam temperatures in the range of 700{degree}C. The principal efforts are based on development of creep resistent nickel-based materials named super-alloys for the hottest areas of the water/steam cycle. The Advanced 700{degree}C PF Power Plant project will improve the competitiveness of coal-fired power generation. Furthermore, it will provide a major reduction of CO{sub 2} from coal-fired power plants in the range of 15% from the best PF power plants presently and up to 40% from older plants. The demonstration programme will leave the possibility of any plant output between 400 and 1000 MW. The project will run to the end of 2003. 8 figs.

  9. Advanced Power Plant Development and Analysis Methodologies

    Energy Technology Data Exchange (ETDEWEB)

    A.D. Rao; G.S. Samuelsen; F.L. Robson; B. Washom; S.G. Berenyi

    2006-06-30

    Under the sponsorship of the U.S. Department of Energy/National Energy Technology Laboratory, a multi-disciplinary team led by the Advanced Power and Energy Program of the University of California at Irvine is defining the system engineering issues associated with the integration of key components and subsystems into advanced power plant systems with goals of achieving high efficiency and minimized environmental impact while using fossil fuels. These power plant concepts include 'Zero Emission' power plants and the 'FutureGen' H2 co-production facilities. The study is broken down into three phases. Phase 1 of this study consisted of utilizing advanced technologies that are expected to be available in the 'Vision 21' time frame such as mega scale fuel cell based hybrids. Phase 2 includes current state-of-the-art technologies and those expected to be deployed in the nearer term such as advanced gas turbines and high temperature membranes for separating gas species and advanced gasifier concepts. Phase 3 includes identification of gas turbine based cycles and engine configurations suitable to coal-based gasification applications and the conceptualization of the balance of plant technology, heat integration, and the bottoming cycle for analysis in a future study. Also included in Phase 3 is the task of acquiring/providing turbo-machinery in order to gather turbo-charger performance data that may be used to verify simulation models as well as establishing system design constraints. The results of these various investigations will serve as a guide for the U. S. Department of Energy in identifying the research areas and technologies that warrant further support.

  10. Advanced Power Plant Development and Analyses Methodologies

    Energy Technology Data Exchange (ETDEWEB)

    G.S. Samuelsen; A.D. Rao

    2006-02-06

    Under the sponsorship of the U.S. Department of Energy/National Energy Technology Laboratory, a multi-disciplinary team led by the Advanced Power and Energy Program of the University of California at Irvine is defining the system engineering issues associated with the integration of key components and subsystems into advanced power plant systems with goals of achieving high efficiency and minimized environmental impact while using fossil fuels. These power plant concepts include ''Zero Emission'' power plants and the ''FutureGen'' H{sub 2} co-production facilities. The study is broken down into three phases. Phase 1 of this study consisted of utilizing advanced technologies that are expected to be available in the ''Vision 21'' time frame such as mega scale fuel cell based hybrids. Phase 2 includes current state-of-the-art technologies and those expected to be deployed in the nearer term such as advanced gas turbines and high temperature membranes for separating gas species and advanced gasifier concepts. Phase 3 includes identification of gas turbine based cycles and engine configurations suitable to coal-based gasification applications and the conceptualization of the balance of plant technology, heat integration, and the bottoming cycle for analysis in a future study. Also included in Phase 3 is the task of acquiring/providing turbo-machinery in order to gather turbo-charger performance data that may be used to verify simulation models as well as establishing system design constraints. The results of these various investigations will serve as a guide for the U. S. Department of Energy in identifying the research areas and technologies that warrant further support.

  11. Advances in Research on Genetically Engineered Plants for Metal Resistance

    Institute of Scientific and Technical Information of China (English)

    Ri-Qing Zhang; Chun-Fang Tang; Shi-Zhi Wen; Yun-Guo Liu; Ke-Lin Li

    2006-01-01

    The engineering application of natural hyperaccumulators in removing or inactivating metal pollutants from soil and surface water in field trials mostly presents the insurmountable shortcoming of low efficiency owing to their little biomass and slow growth. Based on further understanding of the molecular mechanism of metal uptake, translocation, and also the separation, identification, and cloning of some related functional genes, this article highlights and summarizes in detail the advances in research on transgenic techniques, such as Agrobacterium tumefaciens-mediated transformation and particle bombardment, in breeding of plants for metal resistance and accumulation, and points out that deepening the development of transgenic plants is one of the efficient approaches to improving phytoremediation efficiency of metal-contaminated environments. From the viewpoint of sustainable development, governments should strengthen support to the development of genetic engineering for metal resistance and accumulation in plants.

  12. Research advances on transgenic plant vaccines.

    Science.gov (United States)

    Han, Mei; Su, Tao; Zu, Yuan-Gang; An, Zhi-Gang

    2006-04-01

    In recent years, with the development of genetics molecular biology and plant biotechnology, the vaccination (e.g. genetic engineering subunit vaccine, living vector vaccine, nucleic acid vaccine) programs are taking on a prosperous evolvement. In particular, the technology of the use of transgenic plants to produce human or animal therapeutic vaccines receives increasing attention. Expressing vaccine candidates in vegetables and fruits open up a new avenue for producing oral/edible vaccines. Transgenic plant vaccine disquisitions exhibit a tempting latent exploiting foreground. There are a lot of advantages for transgenic plant vaccines, such as low cost, easiness of storage, and convenient immune-inoculation. Some productions converged in edible tissues, so they can be consumed directly without isolation and purification. Up to now, many transgenic plant vaccine productions have been investigated and developed. In this review, recent advances on plant-derived recombinant protein expression systems, infectious targets, and delivery systems are presented. Some issues of high concern such as biosafety and public health are also discussed. Special attention is given to the prospects and limitations on transgenic plant vaccines.

  13. Advances on Plant Pathogenic Mycotoxin Binding Proteins

    Institute of Scientific and Technical Information of China (English)

    WANG Chao-hua; DONG Jin-gao

    2002-01-01

    Toxin-binding protein is one of the key subjects in plant pathogenic mycotoxin research. In this paper, new advances in toxin-binding proteins of 10 kinds of plant pathogenic mycotoxins belonging to Helminthosporium ,Alternaria ,Fusicoccum ,Verticillium were reviewed, especially the techniques and methods of toxin-binding proteins of HS-toxin, HV-toxin, HMT-toxin, HC-toxin. It was proposed that the isotope-labeling technique and immunological chemistry technique should be combined together in research of toxin-binding protein, which will be significant to study the molecular recognition mechanism between host and pathogenic fungus.

  14. Transformation and regeneration of the holoparasitic plant Phelipanche aegyptiaca

    Directory of Open Access Journals (Sweden)

    Fernández-Aparicio Mónica

    2011-11-01

    Full Text Available Abstract Background Transformation and subsequent regeneration of holoparasitic plants has never been reported, in part due to challenges in developing transformation protocols, but also because regeneration of obligate parasites is difficult since their survival depends completely on successful haustorium penetration of a host and the formation of vascular connections. The recent completion of a massive transcriptome sequencing project (the Parasitic Plant Genome Project will fuel the use of genomic tools for studies on parasitic plants. A reliable system for holoparasite transformation is needed to realize the full value of this resource for reverse genetics and functional genomics studies. Results Here we demonstrate that transformation of Phelipanche aegyptiaca is achieved by infection of 3 month-old in vitro grown P. aegyptiaca calli with Agrobacterium rhizogenes harboring the yellow fluorescent protein (YFP. Four months later, YFP-positive regenerated calli were inoculated onto tomato plants growing in a minirhizotron system. Eight days after inoculation, transgenic parasite tissue formed lateral haustoria that penetrated the host and could be visualized under UV illumination through intact host root tissue. YFP-positive shoot buds were observed one month after inoculation. Conclusions This work constitutes a breakthrough in holoparasitic plant research methods. The method described here is a robust system for transformation and regeneration of a holoparasitic plant and will facilitate research on unique parasitic plant capabilities such as host plant recognition, haustorial formation, penetration and vascular connection.

  15. Advanced Gasifier Pilot Plant Concept Definition

    Energy Technology Data Exchange (ETDEWEB)

    Steve Fusselman; Alan Darby; Fred Widman

    2005-08-31

    This report presents results from definition of a preferred commercial-scale advanced gasifier configuration and concept definition for a gasification pilot plant incorporating those preferred technologies. The preferred commercial gasifier configuration was established based on Cost Of Electricity estimates for an IGCC. Based on the gasifier configuration trade study results, a compact plug flow gasifier, with a dry solids pump, rapid-mix injector, CMC liner insert and partial quench system was selected as the preferred configuration. Preliminary systems analysis results indicate that this configuration could provide cost of product savings for electricity and hydrogen ranging from 15%-20% relative to existing gasifier technologies. This cost of product improvement draws upon the efficiency of the dry feed, rapid mix injector technology, low capital cost compact gasifier, and >99% gasifier availability due to long life injector and gasifier liner, with short replacement time. A pilot plant concept incorporating the technologies associated with the preferred configuration was defined, along with cost and schedule estimates for design, installation, and test operations. It was estimated that a 16,300 kg/day (18 TPD) pilot plant gasifier incorporating the advanced gasification technology and demonstrating 1,000 hours of hot-fire operation could be accomplished over a period of 33 months with a budget of $25.6 M.

  16. Recent Advances in Ocean Nuclear Power Plants

    Directory of Open Access Journals (Sweden)

    Kang-Heon Lee

    2015-10-01

    Full Text Available In this paper, recent advances in Ocean Nuclear Power Plants (ONPPs are reviewed, including their general arrangement, design parameters, and safety features. The development of ONPP concepts have continued due to initiatives taking place in France, Russia, South Korea, and the United States. Russia’s first floating nuclear power stations utilizing the PWR technology (KLT-40S and the spar-type offshore floating nuclear power plant designed by a research group in United States are considered herein. The APR1400 and SMART mounted Gravity Based Structure (GBS-type ONPPs proposed by a research group in South Korea are also considered. In addition, a submerged-type ONPP designed by DCNS of France is taken into account. Last, issues and challenges related to ONPPs are discussed and summarized.

  17. VIP1: linking Agrobacterium-mediated transformation to plant immunity?

    Science.gov (United States)

    Liu, Yukun; Kong, Xiangpei; Pan, Jiaowen; Li, Dequan

    2010-08-01

    Agrobacterium tumefaciens is the most efficient vehicle used today for the production of transgenic plants and plays an essential role in basic scientific research and in agricultural biotechnology. Previously, plant VirE2-interacting protein 1 (VIP1) was shown to play a role in Agrobacterium-mediated transformation. Recent reports demonstrate that VIP1, as one of the bZIP transcription factors, is also involved in plant immunity responses. Agrobacterium is able to activate and abuse VIP1 for transformation. These findings highlight Agrobacterium-host interaction and unveil how Agrobacterium hijacks host cellular mechanism for its own benefit. This review focuses on the roles played by VIP1 in Agrobacterium-mediated transformation and plant immunity.

  18. Genetic transformation of plants by protoplast electroporation.

    Science.gov (United States)

    Bates, G W

    1994-10-01

    This article describes an optimized protocol for the electroporation of tobacco mesophyll protoplasts together with notes and data on the effects of various parameters and suggestions for work with protoplasts of other species. In this protocol, electroporation is achieved by means of electrical pulses from a high-voltage, capacitive-discharge unit. Procedures are described for measurement of protoplast viability with Evan's blue, the detection of transient expression of CAT and GUS gene plasmid constructs, and for the recovery of stable transformants based on selection for kanamycin resistance.

  19. Cold tolerance of potato plants transformed with yeast invertase gene

    Directory of Open Access Journals (Sweden)

    Alexander N. Deryaabin

    2013-12-01

    Full Text Available Our study was carried out with potato plants (Solanum tuberosun L.,cv. Désirée transformed with the yeast invertase gene under the control of the B33 class I patatin promoter and with the proteinase inhibitor II leader peptide sequence providing for the apoplastic enzyme localization (B33-inv plants and with the plants transformed with the reporter gene encoding bb-glucuronidase under the control of the 35S CaMV promoter (control plants. Exposure to 5°C during 6 days caused an increase in invertase activity and sugar content in B33-inv leaves in comparison with the control plants. Cell membranes of B33-inv plant cells showed greater cold tolerance under low temperature conditions than control plants that was recorded by electrolyte release. We supposed that higher cold tolerance of B33-inv plants was caused by stabilizing effect of sugar on the membranes, because B33-inv plants differ from the control plants in higher invertase activity, induced by expression of yeast invertase gene, and high content of sugars.

  20. NSF ADVANCE: Institutional Transformation to Achieve Faculty Diversity

    Science.gov (United States)

    Anthony, E. Y.

    2004-12-01

    The NSF ADVANCE initiative is designed to enhance gender equity in academic science and engineering faculty. One of its components - Institutional Transformation - has the goal of establishing strategies and policies that will revolutionize institutional climate so that diverse faculty flourish. The University of Texas at El Paso is one of 19 institutions to currently hold a 5-year grant under the Institutional Transformation program. This poster presentation highlights practices from the participating institutions. Two general aspects of the program are: 1) co-principal investigators are a blend of administrators and active researchers. This blend ensures a bottom-up, top-down approach to presenting gender equity to faculty. 2) Many of the investigators have diversity as their research focus, which is intended to result in rigorous, peer-reviewed dissemination of institutional results. Specific effors for all institutions relate to recruitment, retention, and advancement of female faculty and, by establishing equitable conditions, to improvement of the workplace for all faculty. To aid recruitment, institutions have committed faculty involved in the search process, including training of search committees in diversity strategies and interaction with candidates. A close working relationship with the campus EO officer is essential. Retention strategies center on mentoring, monetary support for research, and policy implementation. Policies focus on work-family balance. Advancement of females to important administrative and non-administrative leadership roles is the third focus. Workshops and seminars on leadership skills are common in the various institutions. Finally, a central theme of the program is that, in addition to specific strategies, institutions must articulate diversity as a core value and reflect on the means to actualize this value. More information on the NSF ADVANCE program, including links to the Institutional Transformation grantees, may be found on

  1. Plant regeneration and genetic transformation in switchgrass-A review

    Institute of Scientific and Technical Information of China (English)

    Paul Merrick; Shuizhang Fei

    2015-01-01

    Switchgrass is native to the tal grass prairie of North America. It is self-incompatible and has varied ploidy levels from diploid (2x) to dodecaploid (12x) with tetraploid and octoploid being the most common. The high yielding potential and the ability to grow wel in marginal lands make switchgrass an ideal species as a dedicated biomass producer for lignocel ulosic ethanol production. Genetic transformation is an important tool for studying gene function and for germplasm improvement in switchgrass, the genome of which has been sequenced recently. This paper intends to provide a comprehensive review on plant regeneration and genetic transformation in switchgrass. We ifrst reviewed the effect of explants, basal medium and plant growth regulators on plant regeneration in switchgrass, which is a prerequisite for genetic transformation. We then reviewed the progresses on genetic transformation with either the biolistic or Agrobacterium-mediated method in switchgrass, and discussed various techniques employed to improve the transformation efifciency. Final y we reviewed the recent progresses on the use of genetic transformation in improving biomass quality such as the reduction of lignin, and in increasing biomass yield in switchgrass. We also provided a future perspective on the use of new genome editing technologies in switchgrass and its potential impact on regulatory processes.

  2. Novel and potential application of cryopreservation to plant genetic transformation.

    Science.gov (United States)

    Wang, Biao; Zhang, Zhibo; Yin, Zhenfang; Feng, Chaohong; Wang, Qiaochun

    2012-01-01

    The world population now is 6.7 billion and is predicted to reach 9 billion by 2050. Such a rapid growing population has tremendously increased the challenge for food security. Obviously, it is impossible for traditional agriculture to ensure the food security, while plant biotechnology offers considerable potential to realize this goal. Over the last 15 years, great benefits have been brought to sustainable agriculture by commercial cultivation of genetically modified (GM) crops. Further development of new GM crops will with no doubt contribute to meeting the requirements for food by the increasing population. The present article provides updated comprehensive information on novel and potential application of cryopreservation to genetic transformation. The major progresses that have been achieved in this subject include (1), long-term storage of a large number of valuable plant genes, which offers a good potential for further development of novel cultivars by genetic transformation; (2), retention of regenerative capacity of embryogenic tissues and protoplasts, which ensures efficient plant regeneration system for genetic transformation; (3), improvement of transformation efficiency and plant regeneration of transformed cells; (4), long-term preservation of transgenic materials with stable expression of transgenes and productive ability of recombinant proteins, which allows transgenic materials to be stored in a safe manner before being analyzed and evaluated, and allows establishment of stable seed stocks for commercial production of homologous proteins. Data provided in this article clearly demonstrate that cryo-technique has an important role to play in the whole chain of genetic transformation. Further studies coupling cryotechnique and genetic transformation are expected to significantly improve development of new GM crops.

  3. Plant Products for Pharmacology: Application of Enzymes in Their Transformations

    Directory of Open Access Journals (Sweden)

    Marie Zarevúcka

    2008-12-01

    Full Text Available Different plant products have been subjected to detailed investigations due to their increasing importance for improving human health. Plants are sources of many groups of natural products, of which large number of new compounds has already displayed their high impact in human medicine. This review deals with the natural products which may be found dissolved in lipid phase (phytosterols, vitamins etc.. Often subsequent convenient transformation of natural products may further improve the pharmacological properties of new potential medicaments based on natural products. To respect basic principles of sustainable and green procedures, enzymes are often employed as efficient natural catalysts in such plant product transformations. Transformations of lipids and other natural products under the conditions of enzyme catalysis show increasing importance in environmentally safe and sustainable production of pharmacologically important compounds. In this review, attention is focused on lipases, efficient and convenient biocatalysts for the enantio- and regioselective formation / hydrolysis of ester bond in a wide variety of both natural and unnatural substrates, including plant products, eg. plant oils and other natural lipid phase compounds. The application of enzymes for preparation of acylglycerols and transformation of other natural products provides big advantage in comparison with employing of conventional chemical methods: Increased selectivity, higher product purity and quality, energy conservation, elimination of heavy metal catalysts, and sustainability of the employed processes, which are catalyzed by enzymes. Two general procedures are used in the transformation of lipid-like natural products: (a Hydrolysis/alcoholysis of triacylglycerols and (b esterification of glycerol. The reactions can be performed under conventional conditions or in supercritical fluids/ionic liquids. Enzyme-catalyzed reactions in supercritical fluids combine the

  4. Biofuel Potential of Plants Transformed Genetically With NAC Family Genes

    Directory of Open Access Journals (Sweden)

    Sadhana eSingh

    2016-01-01

    Full Text Available NAC genes contribute to enhance survivability of plants under conditions of environmental stress and in secondary growth of the plants, thereby building biomass. Thus, genetic transformation of plants using NAC genes provides a possibility to tailor made biofuel plants. Over-expression studies have indicated that NAC family genes can provide tolerance to various biotic and abiotic stresses, either by physiological or biochemical changes at the cellular level, or by affecting visible morphological and anatomical changes, for example by development of lateral roots in a number of plants. Over-expression of these genes also work as triggers for development of secondary cell walls. In our laboratory, we have observed a NAC gene from Lepidium latifolium contributing to both enhanced biomass as well as cold stress tolerance of model plants tobacco. Thus, we have reviewed all the developments of genetic engineering using NAC genes which could enhance the traits required for biofuel plants, either by enhancing the stress tolerance or by enhancing the biomass of the plants. KeywordsNAC, Genetically engineered plants, Abiotic stress tolerance, Secondary growth, Cell wall synthesis, Biomass

  5. Advanced Neutron Source: Plant Design Requirements

    Energy Technology Data Exchange (ETDEWEB)

    1990-07-01

    The Advanced Neutron Source will be a new world-class facility for research using hot, thermal, cold, and ultra-cold neutrons. The heart of the facility will be a 330-MW (fission), heavy-water cooled and heavy-water moderated reactor. The reactor will be housed in a central reactor building, with supporting equipment located in an adjoining reactor support building. An array of cold neutron guides will fan out into a large guide hall, housing about 30 neutron research stations. Appropriate office, laboratory, and shop facilities will be included to provide a complete facility for users. The ANS is scheduled to begin operation at the Oak Ridge National Laboratory early in the next decade. This PDR document defines the plant-level requirements for the design, construction, and operation of ANS. It also defines and provides input to the individual System Design Description (SDD) documents. Together, this PDR document and the set of SDD documents will define and control the baseline configuration of ANS.

  6. Advanced Neutron Source: Plant Design Requirements

    Energy Technology Data Exchange (ETDEWEB)

    1990-07-01

    The Advanced Neutron Source will be a new world-class facility for research using hot, thermal, cold, and ultra-cold neutrons. The heart of the facility will be a 330-MW (fission), heavy-water cooled and heavy-water moderated reactor. The reactor will be housed in a central reactor building, with supporting equipment located in an adjoining reactor support building. An array of cold neutron guides will fan out into a large guide hall, housing about 30 neutron research stations. Appropriate office, laboratory, and shop facilities will be included to provide a complete facility for users. The ANS is scheduled to begin operation at the Oak Ridge National Laboratory early in the next decade. This PDR document defines the plant-level requirements for the design, construction, and operation of ANS. It also defines and provides input to the individual System Design Description (SDD) documents. Together, this PDR document and the set of SDD documents will define and control the baseline configuration of ANS.

  7. Comparison of Soybean Transformation Efficiency and Plant Factors Affecting Transformation during the Agrobacterium Infection Process.

    Science.gov (United States)

    Jia, Yuying; Yao, Xingdong; Zhao, Mingzhe; Zhao, Qiang; Du, Yanli; Yu, Cuimei; Xie, Futi

    2015-01-01

    The susceptibility of soybean genotype to Agrobacterium infection is a key factor for the high level of genetic transformation efficiency. The objective of this study is to evaluate the plant factors related to transformation in cotyledonary nodes during the Agrobacterium infection process. This study selected three genotypes (Williams 82, Shennong 9 and Bert) with high transformation efficiency, which presented better susceptibility to Agrobacterium infection, and three low transformation efficiency genotypes (General, Liaodou 16 and Kottman), which showed a relatively weak susceptibility. Gibberellin (GA) levels and soybean GA20ox2 and CYP707A2 transcripts of high-efficiency genotypes increased and were higher than those of low-efficiency genotypes; however, the opposite performance was shown in abscisic acid (ABA). Higher zeatin riboside (ZR) content and DNA quantity, and relatively higher expression of soybean IPT5, CYCD3 and CYCA3 were obtained in high-efficiency genotypes. High-efficiency genotypes had low methyl jasmonate (MeJA) content, polyphenol oxidase (PPO) and peroxidase (POD) activity, and relatively lower expression of soybean OPR3, PPO1 and PRX71. GA and ZR were positive plant factors for Agrobacterium-mediated soybean transformation by facilitating germination and growth, and increasing the number of cells in DNA synthesis cycle, respectively; MeJA, PPO, POD and ABA were negative plant factors by inducing defence reactions and repressing germination and growth, respectively.

  8. The Plant-Window System: A framework for an integrated computing environment at advanced nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Wood, R.T.; Mullens, J.A. [Oak Ridge National Lab., TN (United States); Naser, J.A. [Electric Power Research Inst., Palo Alto, CA (United States)

    1997-10-01

    Power plant data, and the information that can be derived from it, provide the link to the plant through which the operations, maintenance and engineering staff understand and manage plant performance. The extensive use of computer technology in advanced reactor designs provides the opportunity to greatly expand the capability to obtain, analyze, and present data about the plant to station personnel. However, to support highly efficient and increasingly safe operation of nuclear power plants, it is necessary to transform the vast quantity of available data into clear, concise, and coherent information that can be readily accessed and used throughout the plant. This need can be met by an integrated computer workstation environment that provides the necessary information and software applications, in a manner that can be easily understood and sued, to the proper users throughout the plan. As part of a Cooperative Research and Development Agreement with the Electric Power Research Institute, the Oak Ridge National laboratory has developed functional requirements for a Plant-Wide Integrated Environment Distributed On Workstations (Plant-Window) System. The Plant-Window System (PWS) can serve the needs of operations, engineering, and maintenance personnel at nuclear power stations by providing integrated data and software applications within a common computing environment. The PWS requirements identify functional capabilities and provide guidelines for standardized hardware, software, and display interfaces so as to define a flexible computing environment for both current generation nuclear power plants and advanced reactor designs.

  9. [PLANT GENETIC TRANSFORMATION USING CARBON NANOTUBES FOR DNA DELIVERY].

    Science.gov (United States)

    Burlaka, O M; Pirko, Ya V; Yemets, A I; Blume, Ya B

    2015-01-01

    The possibility of exploiting carbon nanotubes (CNTs)-based nanocarriers to deliver genes into protoplasts, callus and mesophyll explants of plants was examined. Using single-walled CNTs (SWCNTs) at the concentration of 20 μg/ml and multi-walled CNTs (MWCNTs) at the concentration of 15 μg/ml genetic transformation of Nicotiana tabacum L. mesophyll protoplasts with plasmid pGreen 0029 was carried out and transient expression of reporter yfp gene in the protoplasts was observed. Using SWCNTs at the concentration of 40 μg/ml and MWCNTs at the concentration of 30 μg/ml genetic transformation of N. tabacum callus and leaf explants with nptII gene as a part of plasmid pGreen 0029 was carried out. As a result plant regeneration on selective medium containing 50 mg/lkanamycin was shown. SWCNTs-based nanocarriers de-onstrated their appli-ability to transform protoplasts as well as walled plant cells. Whereas, MWCNTs-based nano-arriers were suitable only for transformation of proto-lasts due to the limiting role of cellulose walls in cell penetration.

  10. Classification of explosives transformation products in plant tissue

    Energy Technology Data Exchange (ETDEWEB)

    Larson, S.L.; Jones, R.P. (Army Corps of Engineers, Vicksburg, MI (United States). Waterways Experiment Station); Escalon, L.; Parker, D. (AScI Corp., McLean, VA (United States))

    1999-06-01

    Explosives contamination in surface or groundwater used for the irrigation of food crops and phytoremediation of explosives-contaminated soil or water using plant-assisted biodegradation have brought about concerns as to the fate of explosives in plants. Liquid scintillation counting, high-performance liquid chromatography, and gel permeation chromatography were utilized to characterize explosives (hexahydro-1,3,5-trinitro-1,3,5-triazine and trinitrotoluene) and their metabolites in plant tissues obtained from three separate studies. Analyzing tissues of yellow nutsedge (Cyperus esculentus), corn (Zea mays), lettuce (Lacuta sativa), tomato (Lyopersicum esculentum), radish (Raphanus sativus), and parrot feather (Myriophyllum aquaticum) from three studies where exposure to explosives at nontoxic levels occurred showed that extensive transformation of the explosive contaminant occurred, variations were noted in uptake and transformation between terrestrial and aquatic plants, the products had significantly higher polarity and water solubility than the parent compounds, and the molecular sizes of the transformation products were significantly greater than those of the parent compounds.

  11. Isolation, culture, and transient transformation of plant protoplasts.

    Science.gov (United States)

    Shen, Jinbo; Fu, Jiaxin; Ma, Jin; Wang, Xiangfeng; Gao, Caiji; Zhuang, ChuXiong; Wan, Jianmin; Jiang, Liwen

    2014-06-03

    Transient gene expression in protoplasts, which has been used in several plant species, is an important and versatile tool for rapid functional gene analysis, protein subcellular localization, and biochemical manipulations. This unit describes transient gene expression by electroporation of DNA into protoplasts of Arabidopsis or tobacco suspension-cultured cells and by polyethylene glycol (PEG)-mediated DNA transformation into protoplasts derived from rice leaf sheaths. PEG-mediated DNA transformation for transient gene expression in rice protoplasts in suspension culture is also described as an alternative technique. Methods for collecting intracellular and secreted proteins are also provided.

  12. Spectral Envelope Transformation in Singing Voice for Advanced Pitch Shifting

    Directory of Open Access Journals (Sweden)

    José L. Santacruz

    2016-11-01

    Full Text Available The aim of the present work is to perform a step towards more natural pitch shifting techniques in singing voice for its application in music production and entertainment systems. In this paper, we present an advanced method to achieve natural modifications when applying a pitch shifting process to singing voice by modifying the spectral envelope of the audio excerpt. To this end, an all-pole model has been selected to model the spectral envelope, which is estimated using a constrained non-linear optimization. The analysis of the global variations of the spectral envelope was carried out by identifying changes of the parameters of the model along with the changes of the pitch. With the obtained spectral envelope transformation functions, we applied our pitch shifting scheme to some sustained vowels in order to compare results with the same transformation made by using the Flex Pitch plugin of Logic Pro X and pitch synchronous overlap and add technique (PSOLA. This comparison has been carried out by means of both an objective and a subjective evaluation. The latter was done with a survey open to volunteers on our website.

  13. Plant Virus Metagenomics: Advances in Virus Discovery.

    Science.gov (United States)

    Roossinck, Marilyn J; Martin, Darren P; Roumagnac, Philippe

    2015-06-01

    In recent years plant viruses have been detected from many environments, including domestic and wild plants and interfaces between these systems-aquatic sources, feces of various animals, and insects. A variety of methods have been employed to study plant virus biodiversity, including enrichment for virus-like particles or virus-specific RNA or DNA, or the extraction of total nucleic acids, followed by next-generation deep sequencing and bioinformatic analyses. All of the methods have some shortcomings, but taken together these studies reveal our surprising lack of knowledge about plant viruses and point to the need for more comprehensive studies. In addition, many new viruses have been discovered, with most virus infections in wild plants appearing asymptomatic, suggesting that virus disease may be a byproduct of domestication. For plant pathologists these studies are providing useful tools to detect viruses, and perhaps to predict future problems that could threaten cultivated plants.

  14. Advances in Agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops.

    Science.gov (United States)

    Singh, Roshan Kumar; Prasad, Manoj

    2016-05-01

    Steady increase in global population poses several challenges to plant science research, including demand for increased crop productivity, grain yield, nutritional quality and improved tolerance to different environmental factors. Transgene-based approaches are promising to address these challenges by transferring potential candidate genes to host organisms through different strategies. Agrobacterium-mediated gene transfer is one such strategy which is well known for enabling efficient gene transfer in both monocot and dicots. Due to its versatility, this technique underwent several advancements including development of improved in vitro plant regeneration system, co-cultivation and selection methods, and use of hyper-virulent strains of Agrobacterium tumefaciens harbouring super-binary vectors. The efficiency of this method has also been enhanced by the use of acetosyringone to induce the activity of vir genes, silver nitrate to reduce the Agrobacterium-induced necrosis and cysteine to avoid callus browning during co-cultivation. In the last two decades, extensive efforts have been invested towards achieving efficient Agrobacterium-mediated transformation in cereals. Though high-efficiency transformation systems have been developed for rice and maize, comparatively lesser progress has been reported in other graminaceous crops. In this context, the present review discusses the progress made in Agrobacterium-mediated transformation system in rice, maize, wheat, barley, sorghum, sugarcane, Brachypodium, millets, bioenergy and forage and turf grasses. In addition, it also provides an overview of the genes that have been recently transferred to these graminaceous crops using Agrobacterium, bottlenecks in this technique and future possibilities for crop improvement.

  15. Identification of Ornamental Plant Functioned as Medicinal Plant Based on Redundant Discrete Wavelet Transformation

    Directory of Open Access Journals (Sweden)

    Kohei Arai

    2013-03-01

    Full Text Available Human has a duty to preserve the nature. One of the examples is preserving the ornamental plant. Huge economic value of plant trading, escalating esthetical value of one space and medicine efficacy that contained in a plant are some positive values from this plant. However, only few people know about its medicine efficacy. Considering the easiness to obtain and the medicine efficacy, this plant should be an initial treatment of a simple disease or option towards chemical based medicines. In order to let people get acquaint, we need a system that can proper identify this plant. Therefore, we propose to build a system based on Redundant Discrete Wavelet Transformation (RDWT through its leaf. Since its character is translation invariant that able to produce some robust features to identify ornamental plant. This system was successfully resulting 95.83% of correct classification rate.

  16. Diagnostics of the power oil-filled transformer equipment of thermal power plants

    Science.gov (United States)

    Eltyshev, D. K.; Khoroshev, N. I.

    2016-08-01

    Problems concerning improvement of the diagnostics efficiency of the electrical facilities and functioning of the generation and distribution systems through the examples of the power oil-filled transformers, as the responsible elements referring to the electrical part of thermal power plants (TPP), were considered. Research activity is based on the fuzzy logic system allowing working both with statistical and expert information presented in the form of knowledge accumulated during operation of the power oil-filled transformer facilities. The diagnostic algorithm for various types of transformers, with the use of the intellectual estimation model of its thermal state on the basis of the key diagnostic parameters and fuzzy inference hierarchy, was developed. Criteria for taking measures allowing preventing emergencies in the electric power systems were developed. The fuzzy hierarchical model for the state assessment of the power oil-filled transformers of 110 kV, possessing high degree of credibility and setting quite strict requirements to the limits of variables of the equipment diagnostic parameters, was developed. The most frequent defects of the transformer standard elements, related with the disturbance of the isolation properties and instrumentation operation, were revealed after model testing on the real object. Presented results may be used both for the express diagnostics of the transformers state without disconnection from the power line and for more detailed analysis of the defects causes on the basis of the advanced list of the diagnostic parameters; information on those parameters may be received only after complete or partial disconnection.

  17. Advances in imaging RNA in plants

    DEFF Research Database (Denmark)

    Christensen, Nynne Meyn; Oparka, Karl J.; Tilsner, Jens

    2010-01-01

    Increasing evidence shows that many RNAs are targeted to specific locations within cells, and that RNA-processing pathways occur in association with specific subcellular structures. Compartmentation of mRNA translation and RNA processing helps to assemble large RNA–protein complexes, while RNA...... targeting allows local protein synthesis and the asymmetric distribution of transcripts during cell polarisation. In plants, intercellular RNA trafficking also plays an additional role in plant development and pathogen defence. Methods that allow the visualisation of RNA sequences within a cellular context......, and preferably at subcellular resolution, can help to answer important questions in plant cell and developmental biology. Here, we summarise the approaches currently available for localising RNA in vivo and address the specific limitations inherent with plant systems....

  18. Advances on Bioactive Polysaccharides from Medicinal Plants.

    Science.gov (United States)

    Xie, Jian-Hua; Jin, Ming-Liang; Morris, Gordon A; Zha, Xue-Qiang; Chen, Han-Qing; Yi, Yang; Li, Jing-En; Wang, Zhi-Jun; Gao, Jie; Nie, Shao-Ping; Shang, Peng; Xie, Ming-Yong

    2016-07-29

    In recent decades, the polysaccharides from the medicinal plants have attracted a lot of attention due to their significant bioactivities, such as anti-tumor activity, antioxidant activity, anticoagulant activity, antidiabetic activity, radioprotection effect, anti-viral activity, hypolipidemic and immunomodulatory activities, which make them suitable for medicinal applications. Previous studies have also shown that medicinal plant polysaccharides are non-toxic and show no side effects. Based on these encouraging observations, most researches have been focusing on the isolation and identification of polysaccharides, as well as their bioactivities. A large number of bioactive polysaccharides with different structural features and biological effects from medicinal plants have been purified and characterized. This review provides a comprehensive summary of the most recent developments in physiochemical, structural features and biological activities of bioactive polysaccharides from a number of important medicinal plants, such as polysaccharides from Astragalus membranaceus, Dendrobium plants, Bupleurum, Cactus fruits, Acanthopanax senticosus, Angelica sinensis (Oliv.) Diels, Aloe barbadensis Miller, and Dimocarpus longan Lour. Moreover, the paper has also been focused on the applications of bioactive polysaccharides for medicinal applications. Recent studies have provided evidence that polysaccharides from medicinal plants can play a vital role in bioactivities. The contents and data will serve as a useful reference material for further investigation, production, and application of these polysaccharides in functional foods and therapeutic agents.

  19. Altered phenotypes in plants transformed with chimeric tobacco peroxidase genes

    Energy Technology Data Exchange (ETDEWEB)

    Lagrimini, L.M.

    1990-12-31

    Peroxidases have been implicated in a variety of secondary metabolic reactions including lignification, cross-linking of cell wall polysaccharides, oxidation of indole-3-acetic acid, regulation of cell elongation, wound-healing, phenol oxidation, and pathogen defense. However, due to the many different isoenzymes and even more potential substrates, it has proven difficult to verify actual physiological roles for peroxidase. We are studying the molecular biology of the tobacco peroxidase genes, and have utilized genetic engineering techniques to produce transgenic plants which differ only in their expression of an individual peroxidase isoenzyme. Many of the in planta functions for any individual isoenzyme may be predicted through the morphological and physiological analysis of transformed plants.

  20. Altered phenotypes in plants transformed with chimeric tobacco peroxidase genes

    Energy Technology Data Exchange (ETDEWEB)

    Lagrimini, L.M.

    1990-01-01

    Peroxidases have been implicated in a variety of secondary metabolic reactions including lignification, cross-linking of cell wall polysaccharides, oxidation of indole-3-acetic acid, regulation of cell elongation, wound-healing, phenol oxidation, and pathogen defense. However, due to the many different isoenzymes and even more potential substrates, it has proven difficult to verify actual physiological roles for peroxidase. We are studying the molecular biology of the tobacco peroxidase genes, and have utilized genetic engineering techniques to produce transgenic plants which differ only in their expression of an individual peroxidase isoenzyme. Many of the in planta functions for any individual isoenzyme may be predicted through the morphological and physiological analysis of transformed plants.

  1. ADVANCING PROTOCOLS FOR POPLARS in vitro PROPAGATION, REGENERATION AND SELECTION OF TRANSFORMANTS

    Directory of Open Access Journals (Sweden)

    Nataliia Kutsokon

    2013-02-01

    Full Text Available Poplars (genus Populus have emerged as a model organism for forest biotechnology, and genetic modification is more advanced for this genus than for any other tree. So far several protocols for microclonal propagation and regeneration for Populus species have been developed. However it is well known that these protocols differ for various species and need to be adapted even for different clones of the same species. This work was focused on developing of protocols for propagation, regeneration and putative transformant´s selection of aspen Populus tremula L. and other two fast-growing Populus species (P. nigra L., P. x canadensis Moench. The regeneration ability for black poplar explants was demonstrated to be three times higher compared to those for aspen and hybrid poplar. It was found that concentration 1 mg/L of phosphinothricin and 25 mg/L of kanamycin is toxic for non- transgenic plant tissues of P. x canadensis and can be applied in transformation experiments when genes of resistance to the corresponding selective agents into the plant genome are introduced.

  2. 草本能源植物培育及催化制备先进液体燃料%Advanced Liquid Fuel Production by Herbage Energy Plant Breeding and Catalytic Transformation

    Institute of Scientific and Technical Information of China (English)

    马隆龙; 刘琪英

    2016-01-01

    协同转化纤维素和半纤维素,实现了糖类衍生物到平台分子(糠醛,HMF和乙酰丙酸)的高效转化,揭示了上述转化过程的反应机理与产物控制机制;设计制备了新型双功能加氢催化剂,研究了水相平台分子HMF加氢氢解为含氧化合物2,5-二甲基呋喃的反应机理、选择性控制规律;制备了高效的氧化催化剂K-OMS-2,阐明了该催化剂上HMF到2,5-呋喃二甲醛的转化规律。发展了从酸水残渣中提取甜高粱木质素的方法,并对其结构进行了表征;在实验室合成了木质素二聚体模型;制备了高效负载型金属催化剂体系,研究了木质素低聚物解聚反应和酚类衍生物制备液体烷烃的反应机理;发展了含钒杂多酸在水/醇混合溶剂体系中催化氧化解聚木质素,获得了含有芳香醛类化合物,研究了Aldol缩合、频哪醇偶联和傅克反应的增碳反应机理,通过催化剂加氢脱氧,实现了由木质素制取C13-C17的液态烃类燃料。%With aiming to the three key scientific issues, this project conducted the production advanced liquid fuels from biomass, and the development is listed as follows: (1) So as to the first key scientific issue, this project conducted the investigation on the the component and construction, and the function and mechansim of the stress-inducible gene during biosynthesis of energy plant such as energy sorghum. The net mediation mechanism of secondary cell wall formation in energy sorghum by biosynthesis was elucidated. The measurement platform and evaluation system for the genetic diversity of plasm germ was established. (2) For the second key scientific issue, this project clarified the decompolymerization mechanism and product controlling pathway of biomass macro-molecules in hydrothermal condition. The structure properties of acid/alkali treated lignin were explained and the dynamical model of the alkali dissolved lignin was built. For

  3. Recent advances in plant cell wall proteomics.

    Science.gov (United States)

    Jamet, Elisabeth; Albenne, Cécile; Boudart, Georges; Irshad, Muhammad; Canut, Hervé; Pont-Lezica, Rafael

    2008-02-01

    The plant extracellular matrix contains typical polysaccharides such as cellulose, hemicelluloses, and pectins that interact to form dense interwoven networks. Plant cell walls play crucial roles during development and constitute the first barrier of defense against invading pathogens. Cell wall proteomics has greatly contributed to the description of the protein content of a compartment specific to plants. Around 400 cell wall proteins (CWPs) of Arabidopsis, representing about one fourth of its estimated cell wall proteome, have been described. The main points to note are that: (i) the diversity of enzymes acting on polysaccharides suggests a great plasticity of cell walls; (ii) CWPs such as proteases, polysaccharide hydrolytic enzymes, and lipases may contribute to the generation of signals; (iii) proteins of unknown functions were identified, suggesting new roles for cell walls. Recently, the characterization of PTMs such as N- and O-glycosylations improved our knowledge of CWP structure. The presence of many glycoside hydrolases and proteases suggests a complex regulation of CWPs involving various types of post-translational events. The first 3-D structures to be resolved gave clues about the interactions between CWPs, or between CWPs and polysaccharides. Future work should include: extracting and identifying CWPs still recalcitrant to proteomics, describing the cell wall interactome, improving quantification, and unraveling the roles of each of the CWPs.

  4. Analysis of morphological traits of bird's foot trefoil plants cv. Bokor transformed with Agrobacterium rhizogenes

    Directory of Open Access Journals (Sweden)

    Nikolić Radomirka

    2005-01-01

    Full Text Available An efficient method for genetic transformation and shoot regeneration was achieved in bird's foot trefoil cv. Bokor using A. rhizogens. The transformed shoots were regenerated on hairy root segments in high frequency. After rooting and acclimation, transformed To plants were grown in experimental field. Analysis of morphological traits and chemical content in ten unintentionally chosen To bird's foot trefoil plants (genotypes no. 2 and no. 5 was performed. They were compared to those of control non-transformed plants. The traits as a number of stems per plant, length of internodes in longest stem, number of flowers per plant and plan high were very significant differed than the same traits in control plants, while there were no significant differences in the leaf area. No signs of the rol genes genotype and "T" phenotype were present. The transformed plants had significantly higher content of cellulose, while the protein and nitrogen contents of are in the range of control plants.

  5. Model-free adaptive control of advanced power plants

    Energy Technology Data Exchange (ETDEWEB)

    Cheng, George Shu-Xing; Mulkey, Steven L.; Wang, Qiang

    2015-08-18

    A novel 3-Input-3-Output (3.times.3) Model-Free Adaptive (MFA) controller with a set of artificial neural networks as part of the controller is introduced. A 3.times.3 MFA control system using the inventive 3.times.3 MFA controller is described to control key process variables including Power, Steam Throttle Pressure, and Steam Temperature of boiler-turbine-generator (BTG) units in conventional and advanced power plants. Those advanced power plants may comprise Once-Through Supercritical (OTSC) Boilers, Circulating Fluidized-Bed (CFB) Boilers, and Once-Through Supercritical Circulating Fluidized-Bed (OTSC CFB) Boilers.

  6. Model-free adaptive control of advanced power plants

    Science.gov (United States)

    Cheng, George Shu-Xing; Mulkey, Steven L.; Wang, Qiang

    2015-08-18

    A novel 3-Input-3-Output (3.times.3) Model-Free Adaptive (MFA) controller with a set of artificial neural networks as part of the controller is introduced. A 3.times.3 MFA control system using the inventive 3.times.3 MFA controller is described to control key process variables including Power, Steam Throttle Pressure, and Steam Temperature of boiler-turbine-generator (BTG) units in conventional and advanced power plants. Those advanced power plants may comprise Once-Through Supercritical (OTSC) Boilers, Circulating Fluidized-Bed (CFB) Boilers, and Once-Through Supercritical Circulating Fluidized-Bed (OTSC CFB) Boilers.

  7. MICROBIAL TRANSFORMATIONS OF RADIONUCLIDES RELEASED FROM NUCLEAR FUEL REPROCESSING PLANTS.

    Energy Technology Data Exchange (ETDEWEB)

    FRANCIS,A.J.

    2006-10-18

    Microorganisms can affect the stability and mobility of the actinides U, Pu, Cm, Am, Np, and the fission products Tc, I, Cs, Sr, released from nuclear fuel reprocessing plants. Under appropriate conditions, microorganisms can alter the chemical speciation, solubility and sorption properties and thus could increase or decrease the concentrations of radionuclides in solution and the bioavailability. Dissolution or immobilization of radionuclides is brought about by direct enzymatic action or indirect non-enzymatic action of microorganisms. Although the physical, chemical, and geochemical processes affecting dissolution, precipitation, and mobilization of radionuclides have been investigated, we have only limited information on the effects of microbial processes. The mechanisms of microbial transformations of the major and minor actinides and the fission products under aerobic and anaerobic conditions in the presence of electron donors and acceptors are reviewed.

  8. Transformation chez les plantes fixatrices d'azote

    OpenAIRE

    Duhoux, Emile; Bogusz, Didier; Franche, Claudine

    1992-01-01

    Les techniques classiques d'amélioration végétale par hybridation sexuée, fusion somatique et mutagénèse artificielle, conduisent à l'introduction, en mélange et de manière aveugle, d'informations génétiques dans la descendance. Or, de plus en plus, on souhaite introduire seulement un ou quelques caractères nouveaux, parfaitement identifiés, sans bouleverser l'architecture génétique globale de la plante. Les transformations génétiques assurent cette opération. Il n'y a pas actuellement de mét...

  9. Emerging Roles of Agrobacterial Plant-Transforming Oncogenes in Plant Defense Reactions

    Science.gov (United States)

    Bulgakov, Victor P.; Inyushkina, Yuliya V.; Gorpenchenko, Tatiana Y.; Koren, Olga G.; Shkryl, Yuri N.; Zhuravlev, Yuri N.

    2009-01-01

    For recent years, engineering plant metabolic pathways by using rol genes looks promising in several aspects. New directions of rol-gene studies are highlighted in this work underlying the unique regulatory properties of the genes. It is known that following agrobacterial infection, the Agrobacterium rhizogenes rolA, rolB and rolC genes are transferred to plant genome, causing tumor formation and hairy root disease. In this report, we show mat these oncogenes are also involved in regulation of plant defense reactions, including the production of secondary metabolites. Situations occur where the rol genes perform their own critical function to regulate secondary metabolism by bypassing upstream plant control mechanisms and directing defense reactions via a "short cut." The rolC gene expressed in transformed plant cells is efficient in establishing an enhanced resistance of host cells to salt and temperature stresses. The emerging complexity of the rol-gene triggered effects and the involvement of signals generated by these genes in basic processes of cell biology such as calcium and ROS signaling indicate that the plant oncogenes, like some animal protooncogenes, use sophisticated strategies to affect cell growth and differentiation. The data raise the intriguing possibility that some components of plant and animal oncogene signaling pathways share common features.

  10. Transformation of pecan and regeneration of transgenic plants.

    Science.gov (United States)

    McGranahan, G H; Leslie, C A; Dandekar, A M; Uratsu, S L; Yates, I E

    1993-09-01

    A gene transfer system developed for walnut (Juglans regia L.) was successfully applied to pecan (Carya illinoensis [Wang] K. Koch). Repetitively embryogenic somatic embryos derived from open-pollinated seed of 'Elliott', 'Wichita', and 'Schley' were co-cultivated with Agrobacterium strain EHA 101/pCGN 7001, which contains marker genes for beta-glucuronidase activity and resistance to kanamycin. Several modifications of the standard walnut transformation techniques were tested, including a lower concentration of kanamycin and a modified induction medium, but these treatments had no measurable effect on efficiency of transformation. Nineteen of the 764 viable inoculated embryos produced transgenic subclones; 13 of these were from the line 'Elliott'6, 3 from 'Schley'5/3, and 3 from 'Wichita'9. Transgenic embryos of 'Wichita'9 germinated most readily and three subclones were successfully micropropagated. Three transgenic plants of one of these subclones were obtained by grafting the tissue cultured shoots to seedling pecan rootstock in the greenhouse. Gene insertion, initially detected by GUS activity, was confirmed by detection of integrated T-DNA sequences using Southern analysis.

  11. Handling Arabidopsis plants: growth, preservation of seeds, transformation, and genetic crosses.

    Science.gov (United States)

    Rivero, Luz; Scholl, Randy; Holomuzki, Nicholas; Crist, Deborah; Grotewold, Erich; Brkljacic, Jelena

    2014-01-01

    Growing healthy plants is essential for the advancement of Arabidopsis thaliana (Arabidopsis) research. Over the last 20 years, the Arabidopsis Biological Resource Center (ABRC) has collected and developed a series of best-practice protocols, some of which are presented in this chapter. Arabidopsis can be grown in a variety of locations, growth media, and environmental conditions. Most laboratory accessions and their mutant or transgenic derivatives flower after 4-5 weeks and set seeds after 7-8 weeks, under standard growth conditions (soil, long day, 23 ºC). Some mutant genotypes, natural accessions, and Arabidopsis relatives require strict control of growth conditions best provided by growth rooms, chambers, or incubators. Other lines can be grown in less-controlled greenhouse settings. Although the majority of lines can be grown in soil, certain experimental purposes require utilization of sterile solid or liquid growth media. These include the selection of primary transformants, identification of homozygous lethal individuals in a segregating population, or bulking of a large amount of plant material. The importance of controlling, observing, and recording growth conditions is emphasized and appropriate equipment required to perform monitoring of these conditions is listed. Proper conditions for seed harvesting and preservation, as well as seed quality control, are also described. Plant transformation and genetic crosses, two of the methods that revolutionized Arabidopsis genetics, are introduced as well.

  12. Software Framework for Advanced Power Plant Simulations

    Energy Technology Data Exchange (ETDEWEB)

    John Widmann; Sorin Munteanu; Aseem Jain; Pankaj Gupta; Mark Moales; Erik Ferguson; Lewis Collins; David Sloan; Woodrow Fiveland; Yi-dong Lang; Larry Biegler; Michael Locke; Simon Lingard; Jay Yun

    2010-08-01

    This report summarizes the work accomplished during the Phase II development effort of the Advanced Process Engineering Co-Simulator (APECS). The objective of the project is to develop the tools to efficiently combine high-fidelity computational fluid dynamics (CFD) models with process modeling software. During the course of the project, a robust integration controller was developed that can be used in any CAPE-OPEN compliant process modeling environment. The controller mediates the exchange of information between the process modeling software and the CFD software. Several approaches to reducing the time disparity between CFD simulations and process modeling have been investigated and implemented. These include enabling the CFD models to be run on a remote cluster and enabling multiple CFD models to be run simultaneously. Furthermore, computationally fast reduced-order models (ROMs) have been developed that can be 'trained' using the results from CFD simulations and then used directly within flowsheets. Unit operation models (both CFD and ROMs) can be uploaded to a model database and shared between multiple users.

  13. Recent Molecular Advances on Downstream Plant Responses to Abiotic Stress

    Directory of Open Access Journals (Sweden)

    Cláudia Regina Batista de Souza

    2012-07-01

    Full Text Available Abiotic stresses such as extremes of temperature and pH, high salinity and drought, comprise some of the major factors causing extensive losses to crop production worldwide. Understanding how plants respond and adapt at cellular and molecular levels to continuous environmental changes is a pre-requisite for the generation of resistant or tolerant plants to abiotic stresses. In this review we aimed to present the recent advances on mechanisms of downstream plant responses to abiotic stresses and the use of stress-related genes in the development of genetically engineered crops.

  14. TRANSFORMATION

    Energy Technology Data Exchange (ETDEWEB)

    LACKS,S.A.

    2003-10-09

    Transformation, which alters the genetic makeup of an individual, is a concept that intrigues the human imagination. In Streptococcus pneumoniae such transformation was first demonstrated. Perhaps our fascination with genetics derived from our ancestors observing their own progeny, with its retention and assortment of parental traits, but such interest must have been accelerated after the dawn of agriculture. It was in pea plants that Gregor Mendel in the late 1800s examined inherited traits and found them to be determined by physical elements, or genes, passed from parents to progeny. In our day, the material basis of these genetic determinants was revealed to be DNA by the lowly bacteria, in particular, the pneumococcus. For this species, transformation by free DNA is a sexual process that enables cells to sport new combinations of genes and traits. Genetic transformation of the type found in S. pneumoniae occurs naturally in many species of bacteria (70), but, initially only a few other transformable species were found, namely, Haemophilus influenzae, Neisseria meningitides, Neisseria gonorrheae, and Bacillus subtilis (96). Natural transformation, which requires a set of genes evolved for the purpose, contrasts with artificial transformation, which is accomplished by shocking cells either electrically, as in electroporation, or by ionic and temperature shifts. Although such artificial treatments can introduce very small amounts of DNA into virtually any type of cell, the amounts introduced by natural transformation are a million-fold greater, and S. pneumoniae can take up as much as 10% of its cellular DNA content (40).

  15. The Advanced BWR Nuclear Plant: Safe, economic nuclear energy

    Energy Technology Data Exchange (ETDEWEB)

    Redding, J.R. [GE Nuclear Energy, San Jose, CA (United States)

    1994-12-31

    The safety and economics of Advanced BWR Nuclear Power Plants are outlined. The topics discussed include: ABWR Programs: status in US and Japan; ABWR competitiveness: safety and economics; SBWR status; combining ABWR and SBWR: the passive ABWR; and Korean/GE partnership.

  16. Recent advances in plant metabolomics and greener pastures.

    Science.gov (United States)

    Sumner, Lloyd W

    2010-01-27

    Metabolomics is an extension of the omics concept and experimental approaches. However, is metabolomics just another trendy omics fashion perturbation or is metabolomics actually delivering novel content and value? This article highlights some recent advances that definitely support the role of plant metabolomics in the movement toward greener pastures.

  17. The advanced main control console for next japanese PWR plants

    Energy Technology Data Exchange (ETDEWEB)

    Tsuchiya, A. [Hokkaido Electric Power Co., Inc., Sapporo (Japan); Ito, K. [Mitsubishi Heavy Industries, Ltd., Nuclear Energy Systems Engineering Center, Yokohama (Japan); Yokoyama, M. [Mitsubishi Electric Corporation, Energy and Industrial Systems Center, Kobe (Japan)

    2001-07-01

    The purpose of the improvement of main control room designing in a nuclear power plant is to reduce operators' workload and potential human errors by offering a better working environment where operators can maximize their abilities. In order to satisfy such requirements, the design of main control board applied to Japanese Pressurized Water Reactor (PWR) type nuclear power plant has been continuously modified and improved. the Japanese Pressurized Water Reactor (PWR) Utilities (Electric Power Companies) and Mitsubishi Group have developed an advanced main control board (console) reflecting on the study of human factors, as well as using a state of the art electronics technology. In this report, we would like to introduce the configuration and features of the Advanced Main Control Console for the practical application to the next generation PWR type nuclear power plants including TOMARI No.3 Unit of Hokkaido Electric Power Co., Inc. (author)

  18. Gasification CFD Modeling for Advanced Power Plant Simulations

    Energy Technology Data Exchange (ETDEWEB)

    Zitney, S.E.; Guenther, C.P.

    2005-09-01

    In this paper we have described recent progress on developing CFD models for two commercial-scale gasifiers, including a two-stage, coal slurry-fed, oxygen-blown, pressurized, entrained-flow gasifier and a scaled-up design of the PSDF transport gasifier. Also highlighted was NETL’s Advanced Process Engineering Co-Simulator for coupling high-fidelity equipment models with process simulation for the design, analysis, and optimization of advanced power plants. Using APECS, we have coupled the entrained-flow gasifier CFD model into a coal-fired, gasification-based FutureGen power and hydrogen production plant. The results for the FutureGen co-simulation illustrate how the APECS technology can help engineers better understand and optimize gasifier fluid dynamics and related phenomena that impact overall power plant performance.

  19. Advances in recurrence and malignant transformation of sinonasal inverted papillomas

    Science.gov (United States)

    Sun, Qingjia; An, Lifeng; Zheng, Jun; Zhu, Dongdong

    2017-01-01

    Sinonasal inverted papilloma (SIP) is a benign tumor of the nasal cavity and sinus. SIP is characterized by aggressive malignant transformation and a high rate of recurrence. Inadequate removal of the tumor during surgery is one of the most significant contributors to SIP recurrence. A growing body of evidence suggests that molecular alteration in SIP, including human papilloma virus infections, single nucleotide polymorphisms of key genes, deregulation of signaling pathways and immunological changes, may lead to SIP occurrence and malignant transformation. However, the extent to which these molecular mechanisms contribute to SIP pathology and transformation remains unclear due to limited research. Further studies are warranted to elucidate the primary dependent factors that contribute to SIP etiology. The present article reviewed risk factors of progression and recurrence of SIP, including outdoor and industrial occupational exposure, smoking, septal deviation, SIP location, recurrent cases, stage of SIP-associated squamous cell carcinoma and choice of surgical method. PMID:28599459

  20. Assessment of transformability of bacteria associated with tomato and potato plants

    NARCIS (Netherlands)

    Overbeek, van L.S.; Ray, J.L.; Elsas, van J.D.

    2007-01-01

    Transformation of plant-associated bacteria by plant DNA has never been demonstrated in agricultural fields. In total 552 bacterial isolates from stems of Ralstonia solanacearum-infected and healthy tomato plants and from stems and leaves of healthy potato plants were tested for natural genetic comp

  1. Targeted Mutagenesis in Plant Cells through Transformation of Sequence-Specific Nuclease mRNA.

    Directory of Open Access Journals (Sweden)

    Thomas J Stoddard

    Full Text Available Plant genome engineering using sequence-specific nucleases (SSNs promises to advance basic and applied plant research by enabling precise modification of endogenous genes. Whereas DNA is an effective means for delivering SSNs, DNA can integrate randomly into the plant genome, leading to unintentional gene inactivation. Further, prolonged expression of SSNs from DNA constructs can lead to the accumulation of off-target mutations. Here, we tested a new approach for SSN delivery to plant cells, namely transformation of messenger RNA (mRNA encoding TAL effector nucleases (TALENs. mRNA delivery of a TALEN pair targeting the Nicotiana benthamiana ALS gene resulted in mutation frequencies of approximately 6% in comparison to DNA delivery, which resulted in mutation frequencies of 70.5%. mRNA delivery resulted in three-fold fewer insertions, and 76% were 10bp. In an effort to increase mutation frequencies using mRNA, we fused several different 5' and 3' untranslated regions (UTRs from Arabidopsis thaliana genes to the TALEN coding sequence. UTRs from an A. thaliana adenine nucleotide α hydrolases-like gene (At1G09740 enhanced mutation frequencies approximately two-fold, relative to a no-UTR control. These results indicate that mRNA can be used as a delivery vehicle for SSNs, and that manipulation of mRNA UTRs can influence efficiencies of genome editing.

  2. Advanced binary geothermal power plants: Limits of performance

    Science.gov (United States)

    Bliem, C. J.; Mines, G. L.

    1991-01-01

    The Heat Cycle Research Program is investigating potential improvements to power cycles utilizing moderate temperature geothermal resources to produce electrical power. Investigations have specifically examined Rankine cycle binary power systems. Binary Rankine cycles are more efficient than the flash steam cycles at moderate resource temperature, achieving a higher net brine effectiveness. At resource conditions similar to those at the Heber binary plant, it has been shown that mixtures of saturated hydrocarbons (alkanes) or halogenated hydrocarbons operating in a supercritical Rankine cycle gave improved performance over Rankine cycles with the pure working fluids executing single or dual boiling cycles or supercritical cycles. Recently, other types of cycles have been proposed for binary geothermal service. The feasible limits on efficiency of a plant given practical limits on equipment performance is explored and the methods used in these advanced concept plants to achieve the maximum possible efficiency are discussed. (Here feasible is intended to mean reasonably achievable and not cost effective.) No direct economic analysis was made because of the sensitivity of economic results to site specific input. The limit of performance of three advanced plants were considered. The performance predictions were taken from the developers of each concept. The advanced plants considered appear to be approaching the feasible limit of performance. Ultimately, the plant designer must weigh the advantages and disadvantages of the the different cycles to find the best plant for a given service. In addition, a standard is presented of comparison of the work which has been done in the Heat Cycle Research Program and in the industrial sector by Exergy, Inc. and Polythermal Technologies.

  3. Economic benefits of plant optimization and advanced fine coal cleaning

    Energy Technology Data Exchange (ETDEWEB)

    Patwardhan, A.; Honaker, R.Q.; Sevim, H. [Engineering Southern Illinois University-Carbondale, Carbondale, IL (United States). Dept. of Mining and Mineral Resources

    2002-07-01

    Advanced fine coal cleaning (AFCC) technologies such as flotation columns provide metallurgical performances that are superior to those obtained by conventional cleaning devices. However, their implementation into operating preparation plants has been limited due to dewatering and handling concerns and the effect on overall plant economics. To evaluate the impacts of AFCC technologies on overall plant performance, a coal plant model has been developed that optimizes the plant operation based on the constant incremental product quality approach while assessing the improvements in plant mass yield. Also, an empirical model relating the probable error of separation (E{sub p}) to the specific gravity of separation (d{sub 50}) and particle size has been developed for simulating unit operation separation performance. From the analysis of two coal processing plants, the installation of flotation columns for treating the fine coal was found to substantially improve plant yield, and, thus, overall mine profitability over a range of product quality. However, the magnitude of the techno-economical benefits was a function of the feed characteristics, especially particle size-by-size washabilities and inherent moisture. 24 refs., 8 figs., 3 tabs.

  4. Soil 35S Transformation and Availability to Plants

    Institute of Scientific and Technical Information of China (English)

    ZHOUWEI; PANJIARONG

    1999-01-01

    Sulfur transformation in 3 soils maintained in a closed incubation system and its availability to plants were investigated using carrier-free 35S-SO42- and 35S-labeled ryegrass straw.For carrier-free Na235SO4 treatment,78%,70%and 64% of 35S applied were found in Ca(H2PO4)2-extractale S fraction,4%,5% and 7% in slowly soluble inorganic S,11%,15%and 18%in C-O-S,5%,7%,and 6% in C-bonded S,and 5%,7% and 6% in unidentified organic S120 days after incubation in black soil,cinnamon soil and chestnut soil,respectively.Most of 35S uptake by plants came from extractable 35SO42-,and little from C-O-35S and C-bonded 35S,In the treatment with 35S-labeled straw,51%,46%and 36% of 35S incorporated were found in Ca(H2PO4)2-extractable S fraction,7%,6% and 7% in slowly soluble inorganic S,13%,15%and 18% in C-O-S,8%,8%and 6% in C-bonded S,and 18%,25%and 35% in unidentified organic S at the end of incubation in above-mentoned three soils,respectively.Higher availability of C-O-35S,C-bonded 35S and unidentified organic 35S from 35S-labeled straw was observed in 35S-labeled straw treatment compared to carrier-free Na235SO4 treatment.

  5. Application of advanced pattern recognition to power plant condition assessment

    Energy Technology Data Exchange (ETDEWEB)

    Caudill, M.B.; Griebenow, R.D.; Hansen, E.J.

    1996-05-01

    As electric utilities strive to become more competitive, efficient operation and maintenance of steam power cycles becomes a greater concern. As part of an aggressive thermal performance monitoring and improvement program, Tri-State Generation and Transmission Association has integrated advanced pattern recognition technology with more conventional heat balance analysis methods to monitor and diagnose plant cycle deficiencies. Advanced pattern recognition (APR) technology is used for continuous monitoring of plant operation. It can quickly diagnose faulty instrumentation and supply accurate replacement values, providing confidence in the data used for daily operating and engineering decisions. Furthermore, it accurately identifies calibration drift, providing Tri-State with the information required to allocate manpower to only those instruments requiring maintenance. APR is also integrated into the data validation and analysis portions of annual thermal performance tests, providing increased confidence in test data and reducing data analysis time. This paper will present the advanced pattern recognition methodology applied at Tri-State and the benefits in optimizing plant operations and assessing power plant performance.

  6. AN ADVANCED SCALE INVARIANT FEATURE TRANSFORM ALGORITHM FOR FACE RECOGNITION

    OpenAIRE

    Mohammad Mohsen Ahmadinejad; Elizabeth Sherly

    2016-01-01

    In computer vision, Scale-invariant feature transform (SIFT) algorithm is widely used to describe and detect local features in images due to its excellent performance. But for face recognition, the implementation of SIFT was complicated because of detecting false key-points in the face image due to irrelevant portions like hair style and other background details. This paper proposes an algorithm for face recognition to improve recognition accuracy by selecting relevant SIFT key-points only th...

  7. Agrobacterium tumefaciens mediated transformation of the oomycete plant pathogen Phytophthora infestans

    NARCIS (Netherlands)

    Vijn, I.; Govers, F.

    2003-01-01

    Agrobacterium tumefaciens is widely used for plant DNA transformation and, more recently, has also been used to transform yeast and filamentous fungi. Here we present a protocol for Agrobacterium-mediated DNA transformation of the oomycete Phytophthora infestans, the causal agent of potato late blig

  8. Comparative Study of Feature Extraction Components from Several Wavelet Transformations for Ornamental Plants

    Directory of Open Access Journals (Sweden)

    Kohei Arai

    2014-02-01

    Full Text Available Human has a duty to preserve the nature, preserving the plant is one of the examples. This research emphasis on ornamental plant that has functionality not only as ornament plant but also as a medicinal plant. Purpose of this research is to find the best of the particular feature extraction components from several wavelet transformations. It consists of Daubechies, Dyadic, and Dual-tree complex wavelet transformation. Dyadic and Dual-tree complex wavelet transformations have shift invariant property. While Daubechies is a standard wavelet transform that widely used for many applications. This comparison is utilizing leaf image datasets from ornamental plants. From the experiments, obtained that best classification performance attained by Dual-tree complex wavelet transformation with 96.66% of overall performance result.

  9. Cogeneration power plant concepts using advanced gas turbines

    Energy Technology Data Exchange (ETDEWEB)

    Huettenhofer, K.; Lezuo, A. [Siemens Power Generation, Erlangen (Germany)

    2001-07-01

    Cogeneration of heat and power (CHP) is undeniably the environmentally most favourable way of making efficient use of energy in the power generation industry. Cogeneration is also particularly appreciated by political decision makers because of its high yield from primary energy sources, and thus its contribution to the protection of the environment and the conservation of resources. Advanced gas turbines, along with an intelligent power plant design consisting of pre-engineered, modular power plant items, will help cogeneration to play an important role in future energy markets also from an economic point of view. (orig.)

  10. Agrobacterium-mediated transformation of plants: Basic principles ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-19

    Oct 19, 2009 ... culture media composition, tissue damage, suppression and elimination of A. .... some chemicals are also some important factors that affect transformation .... mediated transformation of Vicia faba. Mol. Breed. 8: 243-254.

  11. Biological Effects of Potato Plants Transformation with Glucose Oxidase Gene and their Resistance to Hyperthermia

    Directory of Open Access Journals (Sweden)

    O.I. Grabelnych

    2017-02-01

    Full Text Available It is known that regulation of plant tolerance to adverse environmental factors is connected with short term increase of the concentration of endogenous reactive oxygen species (ROS, which are signalling molecules for the induction of protective mechanisms. Introduction and expression of heterologous gox gene, which encodes glucose oxidase enzyme in plant genome, induce constantly higher content of hydrogen peroxide in plant tissues. It is not known how the introduction of native or modified gox gene affects the plant resistance to high-temperature stress, one of the most commonly used model for the study of stress response and thermal tolerance. In this study, we investigated biological effects of transformation and evaluated the resistance to temperature stress of potato plants with altered levels of glucose oxidase expression. Transformation of potato plants by gox gene led to the more early coming out from tuber dormancy of transformed plants and slower growth rate. Transformants containing the glucose oxidase gene were more sensitive to lethal thermal shock (50 °C, 90 min than the transformant with the empty vector (pBI or untransformed plants (CK. Pre-heating of plants at 37 °C significantly weakened the damaging effect of lethal thermal shock. This attenuation was more significant in the non-transformed plants.

  12. Thermoeconomic Analysis of Advanced Solar-Fossil Combined Power Plants

    OpenAIRE

    Yassine Allani; Klaus Ziegler; Daniel Favrat; Malick Kane

    2000-01-01

    Hybrid solar thermal power plants (with parabolic trough type of solar collectors) featuring gas burners and Rankine steam cycles have been successfully demonstrated by California's Solar Electric Generating System (SEGS). This system has been proven to be one of the most efficient and economical schemes to convert solar energy into electricity. Recent technological progress opens interesting prospects for advanced cycle concepts: a) the ISCCS (Integrated Solar Combined Cycle System)...

  13. Materials and welding engineering in advanced coal utilization plants

    Energy Technology Data Exchange (ETDEWEB)

    Schuhmacher, D.; Schulze-Frielinghaus, W.; Puetz, J.; Eichhorn, F.; Gaever, E. van

    1983-08-01

    The authors present the findings of studies on welding methods for high-temperature alloys used in advanced coal gasification plants. They discuss weld preparation, automatic TIG welding, MIG welding (also with pulsed arc) and plasma arc welding. The mechanical properties of welded joints before and after age hardening are investigated, and the results of fatigue and corrosion tests are presented. The welding methods are compared with a view to their suitability for high-temperature materials.

  14. Investigating Agrobacterium-mediated transformation of Verticillium albo-atrum on plant surfaces.

    Directory of Open Access Journals (Sweden)

    Claire J Knight

    Full Text Available BACKGROUND: Agrobacterium tumefaciens has long been known to transform plant tissue in nature as part of its infection process. This natural mechanism has been utilised over the last few decades in laboratories world wide to genetically manipulate many species of plants. More recently this technology has been successfully applied to non-plant organisms in the laboratory, including fungi, where the plant wound hormone acetosyringone, an inducer of transformation, is supplied exogenously. In the natural environment it is possible that Agrobacterium and fungi may encounter each other at plant wound sites, where acetosyringone would be present, raising the possibility of natural gene transfer from bacterium to fungus. METHODOLOGY/PRINCIPAL FINDINGS: We investigate this hypothesis through the development of experiments designed to replicate such a situation at a plant wound site. A. tumefaciens harbouring the plasmid pCAMDsRed was co-cultivated with the common plant pathogenic fungus Verticillium albo-atrum on a range of wounded plant tissues. Fungal transformants were obtained from co-cultivation on a range of plant tissue types, demonstrating that plant tissue provides sufficient vir gene inducers to allow A. tumefaciens to transform fungi in planta. CONCLUSIONS/SIGNIFICANCE: This work raises interesting questions about whether A. tumefaciens may be able to transform organisms other than plants in nature, or indeed should be considered during GM risk assessments, with further investigations required to determine whether this phenomenon has already occurred in nature.

  15. 77 FR 3009 - Knowledge and Abilities Catalog for Nuclear Power Plant Operators: Advanced Boiling Water Reactors

    Science.gov (United States)

    2012-01-20

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Knowledge and Abilities Catalog for Nuclear Power Plant Operators: Advanced Boiling Water Reactors..., ``Knowledge and Abilities Catalog for Nuclear Power Plant Operators: Advanced Boiling Water Reactors.''...

  16. A Simplified Seed Transformation Method for Obtaining Transgenic Brassica napus Plants

    Institute of Scientific and Technical Information of China (English)

    SONG Li; ZHAO De-gang; WU Yong-jun; TIAN Xiao-e

    2009-01-01

    We report here a seed transformation of sonication-assisted,no-tissue culture to rapidly produce transgenic Brassica napus plants.This method comprises the steps of treating seeds by ultrasonic wave,inoculating Agrobacterium tumefaciens with a recombinant ChlFN-a gene and germinating directly of treatment seed on wet filter papers.The obtained transformants were verified by GUS histochemical assay and nested PCR amplification.It suggests that seed transformation has a potential use in genetic transformation of rape.

  17. Prospects for advanced coal-fuelled fuel cell power plants

    Science.gov (United States)

    Jansen, D.; Vanderlaag, P. C.; Oudhuis, A. B. J.; Ribberink, J. S.

    1994-04-01

    As part of ECN's in-house R&D programs on clean energy conversion systems with high efficiencies and low emissions, system assessment studies have been carried out on coal gasification power plants integrated with high-temperature fuel cells (IGFC). The studies also included the potential to reduce CO2 emissions, and to find possible ways for CO2 extraction and sequestration. The development of this new type of clean coal technology for large-scale power generation is still far off. A significant market share is not envisaged before the year 2015. To assess the future market potential of coal-fueled fuel cell power plants, the promise of this fuel cell technology was assessed against the performance and the development of current state-of-the-art large-scale power generation systems, namely the pulverized coal-fired power plants and the integrated coal gasification combined cycle (IGCC) power plants. With the anticipated progress in gas turbine and gas clean-up technology, coal-fueled fuel cell power plants will have to face severe competition from advanced IGCC power plants, despite their higher efficiency.

  18. Multiple host-cell recombination pathways act in Agrobacterium-mediated transformation of plant cells.

    Science.gov (United States)

    Mestiri, Imen; Norre, Frédéric; Gallego, Maria E; White, Charles I

    2014-02-01

    Using floral-dip, tumorigenesis and root callus transformation assays of both germline and somatic cells, we present here results implicating the four major non-homologous and homologous recombination pathways in Agrobacterium-mediated transformation of Arabidopsis thaliana. All four single mutant lines showed similar mild reductions in transformability, but knocking out three of four pathways severely compromised Agrobacterium-mediated transformation. Although integration of T-DNA into the plant genome is severely compromised in the absence of known DNA double-strand break repair pathways, it does still occur, suggesting the existence of other pathways involved in T-DNA integration. Our results highlight the functional redundancy of the four major plant recombination pathways in transformation, and provide an explanation for the lack of strong effects observed in previous studies on the roles of plant recombination functions in transformation.

  19. Transforming the advanced lab: Part I - Learning goals

    Science.gov (United States)

    Zwickl, Benjamin; Finkelstein, Noah; Lewandowski, H. J.

    2012-02-01

    Within the physics education research community relatively little attention has been given to laboratory courses, especially at the upper-division undergraduate level. As part of transforming our senior-level Optics and Modern Physics Lab at the University of Colorado Boulder we are developing learning goals, revising curricula, and creating assessments. In this paper, we report on the establishment of our learning goals and a surrounding framework that have emerged from discussions with a wide variety of faculty, from a review of the literature on labs, and from identifying the goals of existing lab courses. Our goals go beyond those of specific physics content and apparatus, allowing instructors to personalize them to their contexts. We report on four broad themes and associated learning goals: Modeling (math-physics-data connection, statistical error analysis, systematic error, modeling of engineered "black boxes"), Design (of experiments, apparatus, programs, troubleshooting), Communication, and Technical Lab Skills (computer-aided data analysis, LabVIEW, test and measurement equipment).

  20. Underwater Photosynthesis of Submerged Plants – Recent Advances and Methods

    Science.gov (United States)

    Pedersen, Ole; Colmer, Timothy D.; Sand-Jensen, Kaj

    2013-01-01

    We describe the general background and the recent advances in research on underwater photosynthesis of leaf segments, whole communities, and plant dominated aquatic ecosystems and present contemporary methods tailor made to quantify photosynthesis and carbon fixation under water. The majority of studies of aquatic photosynthesis have been carried out with detached leaves or thalli and this selectiveness influences the perception of the regulation of aquatic photosynthesis. We thus recommend assessing the influence of inorganic carbon and temperature on natural aquatic communities of variable density in addition to studying detached leaves in the scenarios of rising CO2 and temperature. Moreover, a growing number of researchers are interested in tolerance of terrestrial plants during flooding as torrential rains sometimes result in overland floods that inundate terrestrial plants. We propose to undertake studies to elucidate the importance of leaf acclimation of terrestrial plants to facilitate gas exchange and light utilization under water as these acclimations influence underwater photosynthesis as well as internal aeration of plant tissues during submergence. PMID:23734154

  1. Underwater photosynthesis of submerged plants – recent advances and methods

    Directory of Open Access Journals (Sweden)

    Ole ePedersen

    2013-05-01

    Full Text Available We describe the general background and the recent advances in research on underwater photosynthesis of leaf segments, whole communities and plant dominated aquatic ecosystems and present contemporary methods tailor made to quantify photosynthesis and carbon fixation under water. The majority of studies of aquatic photosynthesis have been carried out with detached leaves or thalli and this selectiveness influences the perception of the regulation of aquatic photosynthesis. We thus recommend assessing the influence of inorganic carbon and temperature on natural aquatic communities of variable density in addition to studying detached leaves in the scenarios of rising CO2 and temperature. Moreover, a growing number of researchers are interested in tolerance of terrestrial plants during flooding as torrential rains sometimes result in overland floods that inundate terrestrial plants. We propose to undertake studies to elucidate the importance of leaf acclimation of terrestrial plants to facilitate gas exchange and light utilisation under water as these acclimations influence underwater photosynthesis as well as internal aeration of plant tissues during submergence.

  2. Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells.

    Science.gov (United States)

    Buschmann, Henrik

    2016-01-01

    The continuing analysis of plant cell division will require additional protein localization studies. This is greatly aided by GFP-technology, but plant transformation and the maintenance of transgenic lines can present a significant technical bottleneck. In this chapter I describe a method for the Agrobacterium-mediated genetic transformation of tobacco BY-2 cells. The method allows for the microscopic analysis of fluorescence-tagged proteins in dividing cells in within 2 days after starting a coculture. This transient transformation procedure requires only standard laboratory equipment. It is hoped that this rapid method would aid researchers conducting live-cell localization studies in plant mitosis and cytokinesis.

  3. Genome-wide profiling of genetic variation in Agrobacterium-transformed rice plants*#

    Science.gov (United States)

    Li, Wen-xu; Wu, San-ling; Liu, Yan-hua; Jin, Gu-lei; Zhao, Hai-jun; Fan, Long-jiang; Shu, Qing-yao

    2016-01-01

    Agrobacterium-mediated transformation has been widely used in producing transgenic plants, and was recently used to generate “transgene-clean” targeted genomic modifications coupled with the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas9) system. Although tremendous variation in morphological and agronomic traits, such as plant height, seed fertility, and grain size, was observed in transgenic plants, the underlying mechanisms are not yet well understood, and the types and frequency of genetic variation in transformed plants have not been fully disclosed. To reveal the genome-wide variation in transformed plants, we sequenced the genomes of five independent T0 rice plants using next-generation sequencing (NGS) techniques. Bioinformatics analyses followed by experimental validation revealed the following: (1) in addition to transfer-DNA (T-DNA) insertions, three transformed plants carried heritable plasmid backbone DNA of variable sizes (855–5216 bp) and in different configurations with the T-DNA insertions (linked or apart); (2) each transgenic plant contained an estimated 338–1774 independent genetic variations (single nucleotide variations (SNVs) or small insertion/deletions); and (3) 2–6 new Tos17 insertions were detected in each transformed plant, but no other transposable elements or bacterial genomic DNA. PMID:27921404

  4. Requirements for advanced simulation of nuclear reactor and chemicalseparation plants.

    Energy Technology Data Exchange (ETDEWEB)

    Palmiotti, G.; Cahalan, J.; Pfeiffer, P.; Sofu, T.; Taiwo, T.; Wei,T.; Yacout, A.; Yang, W.; Siegel, A.; Insepov, Z.; Anitescu, M.; Hovland,P.; Pereira, C.; Regalbuto, M.; Copple, J.; Willamson, M.

    2006-12-11

    This report presents requirements for advanced simulation of nuclear reactor and chemical processing plants that are of interest to the Global Nuclear Energy Partnership (GNEP) initiative. Justification for advanced simulation and some examples of grand challenges that will benefit from it are provided. An integrated software tool that has its main components, whenever possible based on first principles, is proposed as possible future approach for dealing with the complex problems linked to the simulation of nuclear reactor and chemical processing plants. The main benefits that are associated with a better integrated simulation have been identified as: a reduction of design margins, a decrease of the number of experiments in support of the design process, a shortening of the developmental design cycle, and a better understanding of the physical phenomena and the related underlying fundamental processes. For each component of the proposed integrated software tool, background information, functional requirements, current tools and approach, and proposed future approaches have been provided. Whenever possible, current uncertainties have been quoted and existing limitations have been presented. Desired target accuracies with associated benefits to the different aspects of the nuclear reactor and chemical processing plants were also given. In many cases the possible gains associated with a better simulation have been identified, quantified, and translated into economical benefits.

  5. A novel gateway-compatible binary vector series (PC-GW) for flexible cloning of multiple genes for genetic transformation of plants.

    Science.gov (United States)

    Dalal, Jyoti; Yalamanchili, Roopa; La Hovary, Christophe; Ji, Mikyoung; Rodriguez-Welsh, Maria; Aslett, Denise; Ganapathy, Sowmya; Grunden, Amy; Sederoff, Heike; Qu, Rongda

    2015-09-01

    The rapidly advancing field of plant synthetic biology requires transforming plants with multiple genes. This has sparked a growing interest in flexible plant transformation vectors, which can be used for multi-gene transformations. We have developed a novel binary vector series, named the PC-GW series (GenBank: KP826769-KP826773), for Agrobacterium-mediated plant transformation. The PC-GW vectors use the pCAMBIA vector backbone, and contain NPTII, hpt, bar, mCherry or egfp genes as selectable markers for plant transformation. In a modified multiple cloning site (MCS) of the T-DNA region, we have placed the attR1, attR2 and ccdB sequences for rapid cloning of one to four genes by Gateway™-assisted recombination. In addition, we have introduced four meganuclease sites, and other restriction sites for multi-gene vector construction. Finally, we have placed a CaMV 35S promoter and a 35S terminator on the 5' and 3' ends of the MCS. The CaMV 35S promoter is flanked by PstI restriction sites that can be used to replace it with another promoter sequence if needed. The PC-GW vectors provide choices for selectable markers, cloning methods, and can accommodate up to eight gene constructs in a single T-DNA, thereby significantly reducing the number of transformations or crosses needed to generate multi-transgene expressing plants.

  6. Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants.

    Science.gov (United States)

    Majumdar, Sukanya; Garai, Saraswati; Jha, Sumita

    2011-05-01

    We have developed an efficient transformation system for Bacopa monnieri, an important Indian medicinal plant, using Agrobacterium rhizogenes strains LBA 9402 and A4. Transformed roots induced by strain LBA 9402 spontaneously dedifferentiated to callus while excised roots induced by strain A4 spontaneously showed induction of shoot buds within 10 days. PCR and RT-PCR analysis revealed the presence and expression of the rolAB and rolC genes at the transcription level in pRi A4 transformed cultures indicating that the TL-DNA was integrated retained and expressed in the A4-Ri transformed shoots. Transformed calli showed the presence of rolAB or rol A, TR and ags genes. Transformed plants showed morphological features typically seen in transgenic plants produced by A. rhizogenes. Growth and biomass accumulation was significantly higher in the transformed shoots (twofold) and roots (fourfold) than in the non-transformed (WT) plants. In pRi A4-transformed plants, the content of bacopasaponin D, bacopasaponin F, bacopaside II and bacopaside V was enhanced significantly as compared to WT plants of similar age while bacoside A3 and bacopasaponin C content was comparable with that of WT plants. Significant increase in content of five bacopa saponins could be detected in pRi 9402-transformed callus cultures. There is an overall stimulatory effect on accumulation of bacopa saponins in transformed plants and cells of B. monnieri establishing the role of endogenous elicitation by Ri T-DNA of A. rhizogenes.

  7. Morphological Transformation of Plant Cells in vitro and Its Effect on Plant Growth

    Institute of Scientific and Technical Information of China (English)

    GUO Zhigang; ZENG Zhaolin; LIU Ruizhi; DENG Ying

    2005-01-01

    Enhancement of cell growth in suspension cultures is urgently needed in plant cell culture engineering. This study investigates the relationship between morphological transformation and cell growth in callus and suspension cultures of saffron cells belonging to the cell line C96 induced from Crocus sativus L. In the suspension culture, an unbalanced osmotic pressure between the intracell and extracell regions induced a large morphological transformation which affected normal division of the saffron cells. An increase in osmotic pressure caused by the addition of sucrose inhibits the vacuolation and shrinkage of cytoplasm in the cells. As the sucrose concentration increases, the total amount of accumulated biomass also increases. Besides the sucrose concentration, increased ionic strength and inoculation ratio also help restrain to a large extent the vacuolation and shrinkage of the cytoplasm in the suspended cells, which results in increased biomass. The conditions for optimal biomass are: Murashige and Skoog's (MS) medium with 40 g/L sucrose and 60% (v/v) inoculation ratio.

  8. Transformation products and reaction kinetics of fragrances in advanced wastewater treatment with ozone

    DEFF Research Database (Denmark)

    Janzen, Niklas; Dopp, Elke; Hesse, Julia

    2011-01-01

    ) ethanone (musk ketone/MK), and 1-(2,3,8,8-tetramethyl-1,3,4,5,6,7-hexahydronaphthalen-2-yl)ethanone (OTNE) with ozone in tap water as well as waste water treatment plant (WWTP) effluents is described. Several transformation products are characterized by means of gas chromatography coupled to mass...... spectrometry. One transformation product (HHCB-Lactone) was confirmed by means of a true standard. Musk xylene and musk ketone do not react with ozone under the conditions used in this study. AHTN and HHCB reacted slowly to a multitude of transformation products, while OTNE reacted quickly to several stable...

  9. Thermodynamic analysis of the advanced zero emission power plant

    Directory of Open Access Journals (Sweden)

    Kotowicz Janusz

    2016-03-01

    Full Text Available The paper presents the structure and parameters of advanced zero emission power plant (AZEP. This concept is based on the replacement of the combustion chamber in a gas turbine by the membrane reactor. The reactor has three basic functions: (i oxygen separation from the air through the membrane, (ii combustion of the fuel, and (iii heat transfer to heat the oxygen-depleted air. In the discussed unit hot depleted air is expanded in a turbine and further feeds a bottoming steam cycle (BSC through the main heat recovery steam generator (HRSG. Flue gas leaving the membrane reactor feeds the second HRSG. The flue gas consist mainly of CO2 and water vapor, thus, CO2 separation involves only the flue gas drying. Results of the thermodynamic analysis of described power plant are presented.

  10. Thermodynamic analysis of the advanced zero emission power plant

    Science.gov (United States)

    Kotowicz, Janusz; Job, Marcin

    2016-03-01

    The paper presents the structure and parameters of advanced zero emission power plant (AZEP). This concept is based on the replacement of the combustion chamber in a gas turbine by the membrane reactor. The reactor has three basic functions: (i) oxygen separation from the air through the membrane, (ii) combustion of the fuel, and (iii) heat transfer to heat the oxygen-depleted air. In the discussed unit hot depleted air is expanded in a turbine and further feeds a bottoming steam cycle (BSC) through the main heat recovery steam generator (HRSG). Flue gas leaving the membrane reactor feeds the second HRSG. The flue gas consist mainly of CO2 and water vapor, thus, CO2 separation involves only the flue gas drying. Results of the thermodynamic analysis of described power plant are presented.

  11. Advances in Nitrogen Loss Leached by Precipitation from Plant Canopy

    Institute of Scientific and Technical Information of China (English)

    LI Shi-qing; JI Chun-rong; FANG Ya-ning; CHEN Xiao-li; LI Sheng-xiu

    2008-01-01

    Function of canopy in changing nutrient cycle and flux is one of the focuses in recent years. On the basis of comprehensively appraising published research, we analyzed the nitrogen loss leaching from plant canopy and several factors which affected it. We pointed out the disadvantages of the published researches and the key issues that ought to be solved: (1) The menstruation need to be advanced, and the research should be carried out on nitrogen loss leaching from the canopy of the field plant. (2) If the nitrogen is leached from the plant canopy, the research on the type of nitrogen loss should be carried out, and the nitrogen use efficiency of different varieties should be dealt on a research perspective with regard to the nitrogen leaching. (3) The research should be conducted on the mechanism and pathway, and the progress of nitrogen leaching; and the factors affecting nitrogen leaching should be included in the research, such as the leaf area of different growth stages, stomata densities, stomata conductance, and the apparent free space, which are beneficial to explain the mechanism of nitrogen leaching from the plant canopy.

  12. Novel enabling technologies of gene isolation and plant transformation for improved crop protection

    Energy Technology Data Exchange (ETDEWEB)

    Torok, Tamas

    2013-02-04

    Meeting the needs of agricultural producers requires the continued development of improved transgenic crop protection products. The completed project focused on developing novel enabling technologies of gene discovery and plant transformation to facilitate the generation of such products.

  13. About the safety of power transformers in nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Petrovan Boiarciuc, M.; Muller, S.; Perigaud, G. [Research Department, SERGI Holding, 186, Avenue du General de Gaulle, 78260 Acheres (France)

    2010-07-01

    Oil-filled transformer explosions are due to electrical arcs occurring in transformer tanks. Within milliseconds, arcs vaporize the surrounding oil and the generated gas is quickly pressurized. The pressure difference between the gas bubble and the surrounding liquid oil generates one dynamic pressure peak which propagates and interacts with the tank. Then, the reflections of the pressure peak build up the static pressure, which rises and leads to the tank rupture since tanks are not designed to withstand such levels of static pressure. This results in dangerous explosions, expensive damages and possible environmental pollution. While protective walls surrounding transformers can contain the explosion and sprinklers can fight the induced fire, the current paper presents a strategy to prevent the transformer tank rupture. Once an electrical fault occurs, the fast depressurization of the tank is induced by quick oil evacuation to a reservoir in order to prevent the tank explosion. To evaluate the efficiency of this strategy, experiments and computer simulations are used. The experiments were performed on large scale transformers equipped with the protection. Besides, simulations of the consequences of an electrical arc occurring in a 200 MVA transformer geometry were run and the pressure maps obtained with and without protection were compared. (authors)

  14. Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes

    Institute of Scientific and Technical Information of China (English)

    吕德扬; 曹学远; 唐顺学; 田霞

    2000-01-01

    Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of trans-genie plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties.

  15. Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of transgenic plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties.

  16. Foliage Plant Retrieval using Polar Fourier Transform, Color Moments and Vein Features

    CERN Document Server

    Kadir, Abdul; Susanto, Adhi; Santosa, Paulus Insap

    2011-01-01

    This paper proposed a method that combines Polar Fourier Transform, color moments, and vein features to retrieve leaf images based on a leaf image. The method is very useful to help people in recognizing foliage plants. Foliage plants are plants that have various colors and unique patterns in the leaf. Therefore, the colors and its patterns are information that should be counted on in the processing of plant identification. To compare the performance of retrieving system to other result, the experiments used Flavia dataset, which is very popular in recognizing plants. The result shows that the method gave better performance than PNN, SVM, and Fourier Transform. The method was also tested using foliage plants with various colors. The accuracy was 90.80% for 50 kinds of plants.

  17. Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

    DEFF Research Database (Denmark)

    Trieu, A.T.; Burleigh, S.H.; Kardailsky, I.V.

    2000-01-01

    Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The se...

  18. Recent advances on host plants and expression cassettes' structure and function in plant molecular pharming.

    Science.gov (United States)

    Makhzoum, Abdullah; Benyammi, Roukia; Moustafa, Khaled; Trémouillaux-Guiller, Jocelyne

    2014-04-01

    Plant molecular pharming is a promising system to produce important recombinant proteins such as therapeutic antibodies, pharmaceuticals, enzymes, growth factors, and vaccines. The system provides an interesting alternative method to the direct extraction of proteins from inappropriate source material while offering the possibility to overcome problems related to product safety and source availability. Multiple factors including plant hosts, genes of interest, expression vector cassettes, and extraction and purification techniques play important roles in the plant molecular pharming. Plant species, as a biosynthesis platform, are a crucial factor in achieving high yields of recombinant protein in plant. The choice of recombinant gene and its expression strategy is also of great importance in ensuring a high amount of the recombinant proteins. Many studies have been conducted to improve expression, accumulation, and purification of the recombinant protein from molecular pharming systems. Re-engineered vectors and expression cassettes are also pivotal tools in enhancing gene expression at the transcription and translation level, and increasing protein accumulation, stability, retention and targeting of specific organelles. In this review, we report recent advances and strategies of plant molecular pharming while focusing on the choice of plant hosts and the role of some molecular pharming elements and approaches: promoters, codon optimization, signal sequences, and peptides used for upstream design, purification and downstream processing.

  19. The Process of Transforming an Advanced Lab Course: Goals, Curriculum, and Assessments

    CERN Document Server

    Zwickl, Benjamin M; Lewandowski, H J

    2012-01-01

    A thoughtful approach to designing and improving labs, particularly at the advanced level, is critical for the effective preparation of physics majors for professional work in industry or graduate school. With that in mind, physics education researchers in partnership with the physics faculty at the University of Colorado Boulder have overhauled the senior-level Advanced Physics Lab course. The transformation followed a three part process of establishing learning goals, designing curricula that align with the goals, and assessment. Similar efforts have been carried out in physics lecture courses at the University of Colorado Boulder, but this is the first systematic research-based revision of one of our laboratory courses. The outcomes of this effort include a set of learning goals, a suite of new lab-skill activities and transformed optics labs, and a set of assessments specifically tailored for a laboratory environment. While the particular selection of advanced lab experiments varies widely between institu...

  20. Advanced Thermodynamic Analysis and Evaluation of a Supercritical Power Plant

    Directory of Open Access Journals (Sweden)

    George Tsatsaronis

    2012-06-01

    Full Text Available A conventional exergy analysis can highlight the main components having high thermodynamic inefficiencies, but cannot consider the interactions among components or the true potential for the improvement of each component. By splitting the exergy destruction into endogenous/exogenous and avoidable/unavoidable parts, the advanced exergy analysis is capable of providing additional information to conventional exergy analysis for improving the design and operation of energy conversion systems. This paper presents the application of both a conventional and an advanced exergy analysis to a supercritical coal-fired power plant. The results show that the ratio of exogenous exergy destruction differs quite a lot from component to component. In general, almost 90% of the total exergy destruction within turbines comes from their endogenous parts, while that of feedwater preheaters contributes more or less 70% to their total exergy destruction. Moreover, the boiler subsystem is proven to have a large amount of exergy destruction caused by the irreversibilities within the remaining components of the overall system. It is also found that the boiler subsystem still has the largest avoidable exergy destruction; however, the enhancement efforts should focus not only on its inherent irreversibilities but also on the inefficiencies within the remaining components. A large part of the avoidable exergy destruction within feedwater preheaters is exogenous; while that of the remaining components is mostly endogenous indicating that the improvements mainly depend on advances in design and operation of the component itself.

  1. Morphological changes in atypical bird's foot trefoil plants obtained during genetic transformation by agrobacterium

    Directory of Open Access Journals (Sweden)

    Nikolić Radomirka R.

    2003-01-01

    Full Text Available Atypical plants of bird's foot trefoil (Lotus corniculatus L., Bokor cv showing altered morphological characters that deviate from a normal phenotype were found after plant regeneration from transformed tissue. It had been obtained by genetic transformation of root sections of seedlings using Agrobacterium tumefaciens vector LBA4404/pBI121 on a medium supplemented with 0.2 mg I-1 BAP. The transformants 2b arid 4a were found to have a greatly atypical habit, including shortened internodes, elongated leaves, regular leaf arrangement along the stem and thicker leaves. Inheritance of altered characters was observed in the first progeny generation, and their genetic origin was considered.

  2. Establishment of Genetic Transformation System for Miscanthus sacchariflorus and Obtaining of Its Transgenic Plants

    Institute of Scientific and Technical Information of China (English)

    Yi Zili(易自力); Zhou Puhua; Chu Chengcai; Li Xiang; Tian Wenzhong; Wang Li; Cao Shouyun; Tang Zuoshun

    2004-01-01

    The initiation and regeneration system for embryogenic callus of Miscanthus sacchariflrus is established at very high frequency. Potato proteinaseⅡ(pinⅡ) genes are introduced into the callus of Miscanthus sacchariflorus and some transgenic plants are obtained by the particle bombardment transformation system. PCR and Southern analysis show that target genes are integrated into the genome of these transgenic plants.

  3. Agrobacterium tumefaciens and Agrobacterium rhizogenes transformed roots of the parasitic plant Triphysaria versicolor retain parasitic competence.

    Science.gov (United States)

    Tomilov, Alexey; Tomilova, Natalya; Yoder, John I

    2007-04-01

    Parasitic plants in the Orobanchaceae invade roots of neighboring plants to rob them of water and nutrients. Triphysaria is facultative parasite that parasitizes a broad range of plant species including maize and Arabidopsis. In this paper we describe transient and stable transformation systems for Triphysaria versicolor Fischer and C. Meyer. Agrobacterium tumefaciens and Agrobacterium rhizogenes were both able to transiently express a GUS reporter in Triphysaria seedlings following vacuum infiltration. There was a correlation between the length of time seedlings were conditioned in the dark prior to infiltration and the tissue type transformed. In optimized experiments, nearly all of the vacuum infiltrated seedlings transiently expressed GUS activity in some tissue. Calluses that developed from transformed tissues were selected using non-destructive GUS staining and after several rounds of in vivo GUS selection, we recovered uniformly staining GUS calluses from which roots were subsequently induced. The presence and expression of the transgene in Triphysaria was verified using genomic PCR, RT PCR and Southern hybridizations. Transgenic roots were also obtained by inoculating A. rhizogenes into wounded Triphysaria seedlings. Stable transformed roots were identified using GUS staining or fluorescent microscopy following transformation with vectors containing GFP, dsRED or EYFP. Transgenic roots derived from both A. tumefaciens and A. rhizogenes transformations were morphologically normal and developed haustoria that attached to and invaded lettuce roots. Transgenic roots also remained competent to form haustoria in response to purified inducing factors. These transformation systems will allow an in planta assessment of genes predicted to function in plant parasitism.

  4. Influence of Phosphate on Transformation and Plant Uptake of Cadmium in Cd—amended Soils

    Institute of Scientific and Technical Information of China (English)

    XIONGLI-MING; LURU-KUN

    1991-01-01

    Phosphate was found to have neither influence on Cd transformation nor effect on plant Cd uptake in three Cd-amended upland soils.However,on submerged red earth,high phosphate dressing inhibited the transformation of Cd from exchangeable fraction to other lower-available ones.Cadmium uptake by rice plants increased simultaneously with increasing phosphate supply though plant resistance to Cd also increased at high phosphate level.Application of phosphate as an amendment for Cd-contaminated soil was therefore not recommended in view of the increasing influx of Cd into food chain especially on flooded soils.

  5. A rapid and stable Agrobacterium-mediated transformation method of a medicinal plant Chelone glabra L.

    Science.gov (United States)

    Gao, Zhenrui; Li, Ying; Chen, Jinhua; Chen, Zhixing; Cui, Min-Long

    2015-03-01

    Transformation approach is a useful tool for the study of gene function, the mechanism of molecular regulation, and increase usefulness of components by reverse genetic approach in plants. In this study, we developed a stable and rapid method for Agrobacterium-mediated transformation of a medicinal plant Chelone glabra L. using leaf explants. Stable transformants were obtained using Agrobacterium tumefaciens strains GV2260 and GV3101 that harbored the binary vector pBI121 and contained the neomycin phosphotransferase gene (NPT II) as a selectable marker and a reporter gene β-glucuronidase (GUS). Putative transformants were identified by kanamycin selection and a histochemical assay. PCR and Southern blot analysis confirmed the integration of the GUS gene into transformed genomes as well as detected stable expression of the β-glucuronidase gene (GUS) by RT-PCR. Resulting transformed plants had morphologically normal phenotypes. This method requires two changes of medium and few leaf explants as well as the transformation efficiency of 2-8 % after 2-3 months of inoculation. This method can provide a quick and economical transformation method for reverse genetic approach to change the secondary metabolic pathway to increase useful components in C. glabra.

  6. Lox-dependent gene expression in transgenic plants obtained via Agrobacterium-mediated transformation.

    Science.gov (United States)

    Shcherbak, N; Kishchenko, O; Sakhno, L; Komarnytsky, I; Kuchuk, M

    2013-01-01

    Lox sites of the Cre/lox recombination system from bacteriophage P1 were analyzed for their ability to affect on transgene expression when inserted upstream from a gene coding sequence adjacent to the right border (RB) of T-DNA. Wild and mutated types of lox sites were tested for their effect upon bar gene expression in plants obtained via Agrobacterium-mediated and biolistic transformation methods. Lox-mediated expression of bar gene, recognized by resistance of transgenic plants to PPT, occurred only in plants obtained via Agrobacterium-mediated transformation. RT-PCR analysis confirms that PPT-resistant phenotype of transgenic plants obtained via Agrobacterium-mediated transformation was caused by activation of bar gene. The plasmid with promoterless gus gene together with the lox site adjacent to the RB was constructed and transferred to Nicotiana tabacum as well. Transgenic plants exhibited GUS activity and expression of gus gene was detected in plant leaves. Expression of bar gene from the vectors containing lox site near RB allowed recovery of numerous PPT-resistant transformants of such important crops as Beta vulgaris, Brassica napus, Lactuca sativa and Solanum tuberosum. Our results demonstrate that the lox site sequence adjacent to the RB can be used to control bar gene expression in transgenic plants.

  7. HFE safety reviews of advanced nuclear power plant control rooms

    Science.gov (United States)

    Ohara, John

    1994-01-01

    Advanced control rooms (ACR's) will utilize human-system interface (HSI) technologies that may have significant implications for plant safety in that they will affect the operator's overall role and means of interacting with the system. The Nuclear Regulatory Commission (NRC) reviews the human factors engineering (HFE) aspects of HSI's to ensure that they are designed to good HFE principles and support performance and reliability in order to protect public health and safety. However, the only available NRC guidance was developed more than ten years ago, and does not adequately address the human performance issues and technology changes associated with ACR's. Accordingly, a new approach to ACR safety reviews was developed based upon the concept of 'convergent validity'. This approach to ACR safety reviews is described.

  8. The Recent Advances in Plant Protection Researches of China

    Institute of Scientific and Technical Information of China (English)

    GuoYuyuan; LiangGemei

    2005-01-01

    There are eight examples briefly given in this paper, namely, (1) Polymyxa graminis and the cereal viruses it transmits; (2) the geographical types and facultative migration of cotton bollworm as well as the safety of Bt transgenic cotton; (3) development of crop near-isogenic lines with resistance to diseases; (4) molecular-biological researches induced resistance of rice by infection of blast fungus;(5) to use cytological and molecular-biological techniques for breeding wheat varieties resistant to barley yellow dwarf virus; (6) mass rearing and field releasing of Microplitis mediator for cotton bollworm control; (7) identification and recombination of insecticidal crystal genes of Bacillus thuringiensis; and (8) interplanting of diverse resistance rice varieties for sustainable control of blast disease; which reflect the general situation of recent advances in plant protection researches of China.

  9. Advances on methods for mapping QTL in plant

    Institute of Scientific and Technical Information of China (English)

    ZHANG Yuan-Ming

    2006-01-01

    Advances on methods for mapping quantitative trait loci (QTL) are firstly summarized.Then, some new methods, including mapping multiple QTL, fine mapping of QTL, and mapping QTL for dynamic traits, are mainly described. Finally, some future prospects are proposed, including how to dig novel genes in the germplasm resource, map expression QTL (eQTL) by the use of all markers,phenotypes and micro-array data, identify QTL using genetic mating designs and detect viability loci. The purpose is to direct plant geneticists to choose a suitable method in the inheritance analysis of quantitative trait and in search of novel genes in germplasm resource so that more potential genetic information can be uncovered.

  10. A set of modular plant transformation vectors allowing flexible insertion of up to six expression units.

    Science.gov (United States)

    Goderis, Inge J W M; De Bolle, Miguel F C; François, Isabelle E J A; Wouters, Piet F J; Broekaert, Willem F; Cammue, Bruno P A

    2002-09-01

    We have constructed a binary vector for Agrobacterium-mediated plant transformation, which has a multiple cloning site consisting of 13 hexanucleotide restriction sites, 6 octanucleotide restriction sites and 5 homing endonuclease sites. The homing endonuclease sites have the advantages to be extremely rare in natural sequences and to allow unidirectional cloning. We have also constructed a set of auxiliary vectors allowing the assembly of expression cassettes flanked by homing endonuclease sites. The expression cassettes assembled in these auxiliary vectors can be transferred into the binary vector with virtually no risk of cutting the vector within previously introduced sequences. This vector set is ideally suited for the construction of plant transformation vectors containing multiple expression cassettes and/or other elements such as matrix attachment regions. With this modular vector system, six different expression units were constructed in as many auxiliary vectors and assembled together in one plant transformation vector. The transgenic nature of Arabidopsis thaliana plants, transformed with this plant transformation vector, was assessed and the expression of each of the six genes was demonstrated.

  11. Advanced nuclear power plants for the 21st century

    Energy Technology Data Exchange (ETDEWEB)

    Graham, J. [BNFL, Inc. (United States)

    1996-10-01

    This paper examines, following four issues: capacity; the closure of the fuel cycle; deregulation; and the need to maintain the development of the advanced systems. Demand is a governing parameter: if one doesn`t need the power then there is no need to increase generating capacity. However, there is no question but that the population is growing. All predictions are that new generating capacity will be needed -- the questions are when and how? Until the various issues involved in deregulation are played through it is not clear what form markets will take for the longer term or how investment in large-capital-cost facilities will fit into the financial structure. Deregulation needs the time to throw light on these matters and to gain some experience in the various financial options. The lack of closure of the fuel cycle is both a cost and public perception issue. The US program, as a result of a cold-war paranoia against recycling the partially used fuel, is based upon the final disposal of useful supplies of energy. However, the program itself is plagued with poor management, delays, and uncertainties that are due, in no small measure, to half-uttered thoughts by all concerned, that this is the wrong policy. Current efforts to rethink the policy, and its implementing projects, are welcome. Finally, if it is important to keep design options for advanced nuclear power plants open for the future, then it necessary to maintain valid research and development programs for those designs. Current US policy is damaging to a number of the more advanced options. This paper discusses the candidate systems: LWR, ALMR, HTGR, and CANDU systems for the special contributions they may each provide in an ideal electrical generating industry of the mid-twenty-first century, and makes suggestions for the future. (J.P.N.)

  12. Advanced transformational analysis applied to e-beam proximity effect correction

    Science.gov (United States)

    Barouch, Eytan; Coifman, Ronald R.; Ma, Jimmy T.; Peckerar, Martin C.; Rokhlin, Vladimir

    1993-06-01

    In this paper we address the problem of dose correction in the data bases consistent with ultra- large-scale integration. It is shown that recent advances in transformation theory provide a natural platform on which to build these dose correctors. Specifically, transformation approaches making use of compactly supported, smooth basis functions are shown to be particularly suitable. This is a natural result of the evolution of mathematically based correctors currently in use. Previous work in Parikh, MacDonald and others employed global transform method to determine the values of 'corrected' dose. In most cases, the mathematical inversion is essentially ill posed, in other words, the exact pattern desired cannot be obtained using a finite Gaussian sum. In this paper a set of smooth basis elements of compact support are employed. The mathematically smooth form of the basis makes it easy to match doses at boundaries without Gibbs phenomenon. Thus the transform field can be partitioned for optimum speed. Consequently, while most transformation complexities are of order N6 (the inversion of an N2 X N2 matrix) where N2 is the number of grid points characterizing the database, we developed an algorithm of complexity N2 log N. A method of dose field bias is employed to stem the requests for negative dose. The heart of the numerical process is essentially based on an adapted fast non-uniform-grid Fourier Transform combined with proper filtering and geometric localization methods. Several examples are given.

  13. Thermoeconomic Analysis of Advanced Solar-Fossil Combined Power Plants

    Directory of Open Access Journals (Sweden)

    Yassine Allani

    2000-12-01

    Full Text Available

    Hybrid solar thermal power plants (with parabolic trough type of solar collectors featuring gas burners and Rankine steam cycles have been successfully demonstrated by California's Solar Electric Generating System (SEGS. This system has been proven to be one of the most efficient and economical schemes to convert solar energy into electricity. Recent technological progress opens interesting prospects for advanced cycle concepts: a the ISCCS (Integrated Solar Combined Cycle System that integrates the parabolic trough into a fossil fired combined cycle, which allows a larger exergy potential of the fuel to be converted. b the HSTS (Hybrid Solar Tower System which uses high concentration optics (via a power tower generator and high temperature air receivers to drive the combined cycle power plant. In the latter case, solar energy is used at a higher exergy level as a heat source of the topping cycle. This paper presents the results of a thermoeconomic investigation of an ISCCS envisaged in Tunisia. The study is realized in two phases. In the first phase, a mixed approach, based on pinch technology principles coupled with a mathematical optimization algorithm, is used to minimize the heat transfer exergy losses in the steam generators, respecting the off design operating conditions of the steam turbine (cone law. In the second phase, an economic analysis based on the Levelized Electricity Cost (LEC approach was carried out for the configurations, which provided the best concepts during the first phase. A comparison of ISCCS with pure fossil fueled plants (CC+GT is reported for the same electrical power load. A sensitivity analysis based on the relative size of the solar field is presented.

    •  This paper was presented at the ECOS'00 Conference in Enschede, July 5-7, 2000

  14. Alkene Metathesis and Renewable Materials: Selective Transformations of Plant Oils

    Science.gov (United States)

    Malacea, Raluca; Dixneuf, Pierre H.

    The olefin metathesis of natural oils and fats and their derivatives is the basis of clean catalytic reactions relevant to green chemistry processes and the production of generate useful chemicals from renewable raw materials. Three variants of alkene metathesis: self-metathesis, ethenolysis and cross-metathesis applied to plant oil derivatives will show new routes to fine chemicals, bifunctional products, polymer precursours and industry intermediates.

  15. Recent Advances in Metal-Organic Frameworks for Heterogeneous Catalyzed Organic Transformations

    Energy Technology Data Exchange (ETDEWEB)

    Sabale, Sandip R.; Zheng, Jian; Vemuri, Venkata Rama Ses; Yu, Xiao-Ying; McGrail, Bernard P.; Motkuri, Radha K.

    2016-12-12

    In this review, we have summarized the recent advances in MOF based heterogeneous catalytic chemistry. Catalytic performance of various configurations of MOFs such as active sites, post synthetic modification and MOF derived catalyst, has been summarized in the context of various organic transformation reactions. Post synthetic modification of MOFs via functionalization of organic linkers with active catalytic moieties was deliberated. Also, efficacy of carbonaceous catalysts derived from MOFs was discussed.

  16. SEQUENCES OF PLANT TRANSFORMATION: THE RESULT OF THE INSERTED FOREIGN GENE EXPRESSION OR THE STRESS REACTION?

    Directory of Open Access Journals (Sweden)

    Enikeev A.G.

    2012-08-01

    Full Text Available The gene technology using in plant investigations came to the better understanding of plant physiological processes. At the same time unclear knowledge about transgenic plant physiology may occur a source of incorrect interpretation of obtained results and, consequently, wrong conclusions. In addition, the causes and mechanisms of pleiotropic effects associated with transgenic insertion and gene silencing are remaining unexplained. To solve the problem of transgenic plant physiology it is necessary to pay a close attention to physiological and biochemical peculiarities of plant-agrobacterium symbiosis, because it is a base of plant transformation. It was assumed earlier that agrobacterial transformation is a complex biotic stressing factor and transgenic plant is a long-term stressed organism. We suppose that physiological consequences of plant transformation are determined not only by foreign gene insertion, but largely by stress reaction of plant cells on agrobacterium transformation. Foreign DNA insertion to the plant recipient results in cascade of response reactions remarkably changing metabolism. The degree of such response is supposed to be in dependence on phylogenetic relations of gene donor and recipient. Cell cultures were obtained from tobacco plants (Nicotiana tabacum cv. ’Samsung’ transformed by following Agrobacterium tumefaciens strains: disarmed 669 one and LBA4400 one with hsp 101 in sense or antisense orientation. These cell cultures were used for investigations of the stress-reactions on biotic (bacterial infection agent Clavibacter michiganensis subsp. Sepedonicus and abiotic (high temperature, potassium fluoride factors. It was revealed that “sense” culture was superior to normal and “699” ones in tolerance to pointed stressing factors. Similar results were obtained for “antisense” culture, nevertheless it was a priori not expected to be tolerant. So, to assess the transformation consequence is necessary to

  17. Update on ORNL TRANSFORM Tool: Simulating Multi-Module Advanced Reactor with End-to-End I&C

    Energy Technology Data Exchange (ETDEWEB)

    Hale, Richard Edward [ORNL; Fugate, David L [ORNL; Cetiner, Sacit M [ORNL; Qualls, A L [ORNL

    2015-05-01

    The Small Modular Reactor (SMR) Dynamic System Modeling Tool project is in the fourth year of development. The project is designed to support collaborative modeling and study of various advanced SMR (non-light water cooled reactor) concepts, including the use of multiple coupled reactors at a single site. The focus of this report is the development of a steam generator and drum system model that includes the complex dynamics of typical steam drum systems, the development of instrumentation and controls for the steam generator with drum system model, and the development of multi-reactor module models that reflect the full power reactor innovative small module design concept. The objective of the project is to provide a common simulation environment and baseline modeling resources to facilitate rapid development of dynamic advanced reactor models; ensure consistency among research products within the Instrumentation, Controls, and Human-Machine Interface technical area; and leverage cross-cutting capabilities while minimizing duplication of effort. The combined simulation environment and suite of models are identified as the TRANSFORM tool. The critical elements of this effort include (1) defining a standardized, common simulation environment that can be applied throughout the Advanced Reactors Technology program; (2) developing a library of baseline component modules that can be assembled into full plant models using available geometry, design, and thermal-hydraulic data; (3) defining modeling conventions for interconnecting component models; and (4) establishing user interfaces and support tools to facilitate simulation development (i.e., configuration and parameterization), execution, and results display and capture.

  18. Overexpression of several Arabidopsis histone genes increases agrobacterium-mediated transformation and transgene expression in plants.

    Science.gov (United States)

    Tenea, Gabriela N; Spantzel, Joerg; Lee, Lan-Ying; Zhu, Yanmin; Lin, Kui; Johnson, Susan J; Gelvin, Stanton B

    2009-10-01

    The Arabidopsis thaliana histone H2A-1 is important for Agrobacterium tumefaciens-mediated plant transformation. Mutation of HTA1, the gene encoding histone H2A-1, results in decreased T-DNA integration into the genome of Arabidopsis roots, whereas overexpression of HTA1 increases transformation frequency. To understand the mechanism by which HTA1 enhances transformation, we investigated the effects of overexpression of numerous Arabidopsis histones on transformation and transgene expression. Transgenic Arabidopsis containing cDNAs encoding histone H2A (HTA), histone H4 (HFO), and histone H3-11 (HTR11) displayed increased transformation susceptibility, whereas histone H2B (HTB) and most histone H3 (HTR) cDNAs did not increase transformation. A parallel increase in transient gene expression was observed when histone HTA, HFO, or HTR11 overexpression constructs were cotransfected with double- or single-stranded forms of a gusA gene into tobacco (Nicotiana tabacum) protoplasts. However, these cDNAs did not increase expression of a previously integrated transgene. We identified the N-terminal 39 amino acids of H2A-1 as sufficient to increase transient transgene expression in plants. After transfection, transgene DNA accumulates more rapidly in the presence of HTA1 than with a control construction. Our results suggest that certain histones enhance transgene expression, protect incoming transgene DNA during the initial stages of transformation, and subsequently increase the efficiency of Agrobacterium-mediated transformation.

  19. Educating advanced level practice within complex health care workplace environments through transformational practice development.

    Science.gov (United States)

    Hardy, Sally; Jackson, Carrie; Webster, Jonathan; Manley, Kim

    2013-10-01

    Over the past 20 years health care reform has influenced the development of advanced level practitioner roles and expectations. How advanced level practitioners work to survive the highly stimulating, yet sometimes overwhelming aspects of balancing high quality provision with political reform agendas, amidst economic constraint is considered. Transformational approaches (encompassing education and practice led service development) can provide, promote and 'provoke' a harnessing of complex issues workplace environment to produce creative solutions. Transformational Practice Development provides a structured, rigorous, systematic approach that practitioners, teams and health care consumers alike can utilise to achieve skills and attributes needed for successful innovation. The authors present case study materials from action orientated locally delivered Practice Development, as a complex strategic intervention approach to influence and promote advanced level practice expertise. Initiated through facilitation of transformational leadership, and resultant team based improvements, we present how strategic collaborative processes can harness work chaos and complexity to provide sustainable and productive workplace cultures of effectiveness. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. Selectable genes for transformation of the fungal plant pathogen Glomerella cingulata f. sp. phaseoli (Colletotrichum lindemuthianum).

    Science.gov (United States)

    Rodriguez, R J; Yoder, O C

    1987-01-01

    Glomerella cingulata f. sp. phaseoli (Gcp) was transformed using either of two selectable markers: the amdS + gene of Aspergillus nidulans, which encodes acetamidase and permits growth on acetamide as the sole nitrogen source and the hygBR gene of Escherichia coli which encodes hygromycin B (Hy) phosphotransferase and permits growth in the presence of the antibiotic Hy. The amdS+ gene functioned in Gcp under control of A. nidulans regulatory signals and hygBR was expressed after fusion to a promoter from Cochliobolus heterostrophus, another filamentous ascomycete. Protoplasts to be transformed were generated with the digestive enzyme complex Novozym 234 and then were exposed to plasmid DNA in the presence of 10 mM CaCl2 and polyethylene glycol. Transformation occurred by integration of single or multiple copies of either the amdS+ or hygBR plasmid into the fungal genome. There was no evidence of autonomous plasmid replication. Transformants were mitotically stable on selective and nonselective media. However, transforming DNA in hygBR transformants was observed to occasionally rearrange during nonselective growth, resulting in fewer copies of the plasmid per genome. These transformants were capable of infecting bean (Phaseolus vulgaris), the Gcp host plant, and after recovery from infected tissue were found to have retained both the transforming DNA unrearranged in their genomes and the Hy resistance phenotype. All single-conidial cultures derived from both amdS+ and hygBR transformants had the transplanted phenotype, suggesting that transformants were homokaryons.

  1. Establishment of Plant Acceptor System for Hyperosmosis Transformation.

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Calli and adventitious buds induced from different explants such as young stems and leaves of tomato variety Moneymaker, and mature embryos and young spikelets of rice variety TP309 were used to establish hyperosmotic transformation system at various osmotica treatments.The results revealed that the calli induced from tomato young leaves and rice young spikelets were the ideal transfomation acceptor. The cells of calli were still vigorous when treated wity 0.75 mol/L hyperosmotic solution for 4 hours. The differentiation rates of calli varied from 7.5% to 93.3% in different media. The bud differentiation was apparently inhibited by hyperosmotic treatments. O.75mol/L sucrose hypertonic solution and O.2mol/L CaCl2 solution were favorable hyperosmoticum and hypoosmoticum respectively.

  2. A novel approach to the generation of seamless constructs for plant transformation

    DEFF Research Database (Denmark)

    Kronbak, Remy; Ingvardsen, Christina R.; Madsen, Claus K.;

    2014-01-01

    Background: When creating plant transformation vectors, full control of nucleotides flanking the insert in the final construct may be desirable. Modern ligase-independent methods for DNA-recombination are based on linearization by classical type II restriction endonucleases (REs) alone or in comb......Background: When creating plant transformation vectors, full control of nucleotides flanking the insert in the final construct may be desirable. Modern ligase-independent methods for DNA-recombination are based on linearization by classical type II restriction endonucleases (REs) alone...... on wheat and barley endosperm cells for transient gfp expression.Conclusions: All nucleotides flanking an insert in a biolistic plant transformation vector can be customized by means of SRL in combination with SLIC. Especially type IIS REs promote an efficient cloning result. Based on our findings, we...

  3. Biology and biotechnological advances in Jatropha curcas - A biodiesel plant

    KAUST Repository

    Reddy, Muppala P.

    2009-10-31

    Increasing global demand for energy, the impending depletion of fossil fuels, and concern over global climate change have lead to a resurgence in the development of alternative energy sources. Bio-fuels and bio-energy encompass a wide range of alternative sources of energy of biological origin, and offer excellent, environmentally friendly opportunities to address these issues. The recognition that Jatropha oil can yield high quality biodiesel has led to a surge of interest in Jatropha across the globe, more so in view of the potential for avoiding the dilemma of food vs fuel. Hardiness, rapid growth, easy propagation, short gestation period, wide adaptation, and optimum plant size combine to make this species suitable for sustainable cultivation on wastelands. Besides biodiesel from the seed, the plant produces several useful products that also have commercial value. Large scale cultivation remains the single most important factor that will ultimately determine the success of Jatropha as a source of bio-fuel. The limited knowledge of the genetics of this species, low and inconsistent yields, the narrow genetic variability, and vulnerability to insects and diseases are major constraints in successful cultivation of Jatropha as a bio-fuel crop. Despite the optimal protein content and composition of the pressed cake, the presence of phorbol esters makes it unsuitable for consumption by livestock. A non-toxic variety with low or no phorbol ester content has been identified from Mexico, and the utility of pressed cake from this variety as livestock feed has been demonstrated successfully. In the absence of any morphological differences, identification of linked markers for toxic/non-toxic varieties will add value to the crop and facilitate further improvement. This chapter discusses current efforts towards assessing the diversity and phylogeny of Jatropha, identification of specific markers for toxic and non-toxic varieties, and aspects of micropropagation and genetic

  4. Where Does the Transformation of Precipitated Ceria Nanoparticles in Hydroponic Plants Take Place?

    Science.gov (United States)

    Ma, Yuhui; Zhang, Peng; Zhang, Zhiyong; He, Xiao; Zhang, Junzhe; Ding, Yayun; Zhang, Jing; Zheng, Lirong; Guo, Zhi; Zhang, Lijuan; Chai, Zhifang; Zhao, Yuliang

    2015-09-01

    Cerium oxide nanoparticles (CeO2 NPs) have been found to be partly biotransformed from Ce(IV) to Ce(III) in plants, yet the transformation process and mechanism are not fully understood. Here, we try to clarify the specific site and necessary conditions for the transformation of precipitated CeO2 NPs in hydroponic cucumber plants. Three different treatment modes were adopted according to whether the NPs were incubated with roots all the time or not. Results showed that exposure modes significantly affect the translocation and transformation of CeO2 NPs. In the normal exposure mode, Ce was present as a Ce(IV) and Ce(III) mixture in the roots and shoots, and the proportion of Ce(III) in the shoots was enhanced obviously with the increase of exposure time. The results of short-time incubation and petiole exposure modes suggested that CeO2 NPs could not be reduced within a short incubation time (3 h) or be further reduced inside the plant tissues. It was deduced that root surfaces are the sites, and the physicochemical interaction between the NPs and root exudates at the nanobio interface is the necessary condition for the transformation of CeO2 NPs in plant systems. These results will contribute to understanding the transformation mechanism of CeO2 and other metal-based NPs and properly evaluate their ecological effects.

  5. Micro-Tom Tomato as an Alternative Plant Model System: Mutant Collection and Efficient Transformation.

    Science.gov (United States)

    Shikata, Masahito; Ezura, Hiroshi

    2016-01-01

    Tomato is a model plant for fruit development, a unique feature that classical model plants such as Arabidopsis and rice do not have. The tomato genome was sequenced in 2012 and tomato is becoming very popular as an alternative system for plant research. Among many varieties of tomato, Micro-Tom has been recognized as a model cultivar for tomato research because it shares some key advantages with Arabidopsis including its small size, short life cycle, and capacity to grow under fluorescent lights at a high density. Mutants and transgenic plants are essential materials for functional genomics research, and therefore, the availability of mutant resources and methods for genetic transformation are key tools to facilitate tomato research. Here, we introduce the Micro-Tom mutant database "TOMATOMA" and an efficient transformation protocol for Micro-Tom.

  6. Genetic transformation of the plant pathogens Phytophthora capsici and Phytophthora parasitica.

    Science.gov (United States)

    Bailey, A M; Mena, G L; Herrera-Estrella, L

    1991-08-11

    Phytophthora capsici and P.parasitica were transformed to hygromycin B resistance using plasmids pCM54 and pHL1, which contain the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the Ustilago maydis heat shock hsp70 gene. Enzymes Driselase and Novozyme 234 were used to generate protoplasts which were then transformed following exposure to plasmid DNA and polyethylene glycol 6000. Transformation frequencies of over 500 transformants per micrograms of DNA per 1 x 10(6) protoplasts were obtained. Plasmid pCM54 appears to be transmitted in Phytophthora spp. as an extra-chromosomal element through replication, as shown by Southern blot hybridization and by the loss of plasmid methylation. In addition, transformed strains retained their capacity of infecting Serrano pepper seedlings and Mc. Intosh apple fruits, the host plants for P.capsici and P.parasitica, respectively.

  7. Production of Transgenic Tall Fescue Plants with Enhanced Stress Tolerances by Agrobacterium tumefaciens-Mediated Transformation

    Institute of Scientific and Technical Information of China (English)

    WU Guan-ting; CHEN Jin-qing; HU Zhang-hua; LANG Chun-xiu; CHEN Xiao-yun; WANG Fu-lin; JIN Wei; XIA Ying-wu

    2006-01-01

    In order to improve stress tolerances of turf-type tall fescue (Festuca arundinacea Schreb.), Agrobacterium tumefaciens strain EHA105 carrying plasmid pCMD containing stress tolerance-related CBF1 gene from Arabidopsis thaliana was used to transform mature seeds-derived embryogenic calli of four cultivars. A total of 112 transgenic plants were regenerated from 32 independent lines and verified by histochemical detection of GUS activity, PCR assay and Southern hybridization analysis. The transformation frequency ranged from 0.92 to 2.87% with apparent differences among the cultivars. Stress tolerances of transgenic plants were enhanced, which was shown by the facts that transgenic plants had distinct growth superiority and significantly higher survival rate than non-transformed ones under high salinity and high osmosis stresses,and that relative electronic conductivity of in vitro leaves treated with low and high temperatures, dehydration and high salinity stresses was 25-30% lower in transgenic plants than in control plants. In addition, it was observed that growth of transgenic plants was inhibited due to constitutive overexpression of CBF1 gene under normal environmental conditions.

  8. Bacterial communities involved in sulfur transformations in wastewater treatment plants.

    Science.gov (United States)

    Meyer, Daniel Derrossi; de Andrade, Pedro Avelino Maia; Durrer, Ademir; Andreote, Fernando Dini; Corção, Gertrudes; Brandelli, Adriano

    2016-12-01

    The main sulfate-reducing (SRB) and sulfur-oxidizing bacteria (SOB) in six wastewater treatment plants (WWTPs) located at southern Brazil were described based on high-throughput sequencing of the 16S rDNA. Specific taxa of SRB and SOB were correlated with some abiotic factors, such as the source of the wastewater, oxygen content, sample type, and physical chemical attributes of these WWTPs. When the 22 families of SRB and SOB were clustered together, the samples presented a striking distribution, demonstrating grouping patterns according to the sample type. For SOB, the most abundant families were Spirochaetaceae, Chromatiaceae, Helicobacteriaceae, Rhodospirillaceae, and Neisseriaceae, whereas, for SRB, were Syntrophaceae, Desulfobacteraceae, Nitrospiraceae, and Desulfovibriaceae. The structure and composition of the major families related to the sulfur cycle were also influenced by six chemical attributes (sulfur, potassium, zinc, manganese, phosphorus, and nitrogen). Sulfur was the chemical attribute that most influenced the variation of bacterial communities in the WWTPs (λ = 0.14, p = 0.008). The OTUs affiliated to Syntrophus showed the highest response to the increase of total sulfur. All these findings can contribute to improve the understanding in relation to the sulfur-oxidizing and sulfate-reducing communities in WWTPs aiming to reduce H2S emissions.

  9. Advanced smart tungsten alloys for a future fusion power plant

    Science.gov (United States)

    Litnovsky, A.; Wegener, T.; Klein, F.; Linsmeier, Ch; Rasinski, M.; Kreter, A.; Tan, X.; Schmitz, J.; Mao, Y.; Coenen, J. W.; Bram, M.; Gonzalez-Julian, J.

    2017-06-01

    The severe particle, radiation and neutron environment in a future fusion power plant requires the development of advanced plasma-facing materials. At the same time, the highest level of safety needs to be ensured. The so-called loss-of-coolant accident combined with air ingress in the vacuum vessel represents a severe safety challenge. In the absence of a coolant the temperature of the tungsten first wall may reach 1200 °C. At such a temperature, the neutron-activated radioactive tungsten forms volatile oxide which can be mobilized into atmosphere. Smart tungsten alloys are being developed to address this safety issue. Smart alloys should combine an acceptable plasma performance with the suppressed oxidation during an accident. New thin film tungsten-chromium-yttrium smart alloys feature an impressive 105 fold suppression of oxidation compared to that of pure tungsten at temperatures of up to 1000 °C. Oxidation behavior at temperatures up to 1200 °C, and reactivity of alloys in humid atmosphere along with a manufacturing of reactor-relevant bulk samples, impose an additional challenge in smart alloy development. First exposures of smart alloys in steady-state deuterium plasma were made. Smart tungsten-chroimium-titanium alloys demonstrated a sputtering resistance which is similar to that of pure tungsten. Expected preferential sputtering of alloying elements by plasma ions was confirmed experimentally. The subsequent isothermal oxidation of exposed samples did not reveal any influence of plasma exposure on the passivation of alloys.

  10. Advanced Neutron Source: Plant Design Requirements. Revision 4

    Energy Technology Data Exchange (ETDEWEB)

    1990-07-01

    The Advanced Neutron Source will be a new world-class facility for research using hot, thermal, cold, and ultra-cold neutrons. The heart of the facility will be a 330-MW (fission), heavy-water cooled and heavy-water moderated reactor. The reactor will be housed in a central reactor building, with supporting equipment located in an adjoining reactor support building. An array of cold neutron guides will fan out into a large guide hall, housing about 30 neutron research stations. Appropriate office, laboratory, and shop facilities will be included to provide a complete facility for users. The ANS is scheduled to begin operation at the Oak Ridge National Laboratory early in the next decade. This PDR document defines the plant-level requirements for the design, construction, and operation of ANS. It also defines and provides input to the individual System Design Description (SDD) documents. Together, this PDR document and the set of SDD documents will define and control the baseline configuration of ANS.

  11. Advanced Neutron Source: Plant Design Requirements. Revision 4

    Energy Technology Data Exchange (ETDEWEB)

    1990-07-01

    The Advanced Neutron Source will be a new world-class facility for research using hot, thermal, cold, and ultra-cold neutrons. The heart of the facility will be a 330-MW (fission), heavy-water cooled and heavy-water moderated reactor. The reactor will be housed in a central reactor building, with supporting equipment located in an adjoining reactor support building. An array of cold neutron guides will fan out into a large guide hall, housing about 30 neutron research stations. Appropriate office, laboratory, and shop facilities will be included to provide a complete facility for users. The ANS is scheduled to begin operation at the Oak Ridge National Laboratory early in the next decade. This PDR document defines the plant-level requirements for the design, construction, and operation of ANS. It also defines and provides input to the individual System Design Description (SDD) documents. Together, this PDR document and the set of SDD documents will define and control the baseline configuration of ANS.

  12. The rhizosphere microbiota of plant invaders: an overview of recent advances in the microbiomics of invasive plants.

    Science.gov (United States)

    Coats, Vanessa C; Rumpho, Mary E

    2014-01-01

    Plants in terrestrial systems have evolved in direct association with microbes functioning as both agonists and antagonists of plant fitness and adaptability. As such, investigations that segregate plants and microbes provide only a limited scope of the biotic interactions that dictate plant community structure and composition in natural systems. Invasive plants provide an excellent working model to compare and contrast the effects of microbial communities associated with natural plant populations on plant fitness, adaptation, and fecundity. The last decade of DNA sequencing technology advancements opened the door to microbial community analysis, which has led to an increased awareness of the importance of an organism's microbiome and the disease states associated with microbiome shifts. Employing microbiome analysis to study the symbiotic networks associated with invasive plants will help us to understand what microorganisms contribute to plant fitness in natural systems, how different soil microbial communities impact plant fitness and adaptability, specificity of host-microbe interactions in natural plant populations, and the selective pressures that dictate the structure of above-ground and below-ground biotic communities. This review discusses recent advances in invasive plant biology that have resulted from microbiome analyses as well as the microbial factors that direct plant fitness and adaptability in natural systems.

  13. The rhizosphere microbiota of plant invaders: an overview of recent advances in the microbiomics of invasive plants

    Directory of Open Access Journals (Sweden)

    Vanessa eCoats

    2014-07-01

    Full Text Available Plants in terrestrial systems have evolved in direct association with microbes functioning as both agonists and antagonists of plant fitness and adaptability. As such, investigations that segregate plants and microbes provide only a limited scope of the biotic interactions that dictate plant community structure and composition in natural systems. Invasive plants provide an excellent working model to compare and contrast the effects of microbial communities associated with natural plant populations on plant fitness, adaptation, and fecundity. The last decade of DNA sequencing technology advancements opened the door to microbial community analysis, which has led to an increased awareness of the importance of an organism’s microbiome and the disease states associated with microbiome shifts. Employing microbiome analysis to study the symbiotic networks associated with invasive plants will help us to understand what microorganisms contribute to plant fitness in natural systems, how different soil microbial communities impact plant fitness and adaptability, specificity of host-microbe interactions in natural plant populations, and the selective pressures that dictate the structure of above-ground and below-ground biotic communities. This review discusses recent advances in invasive plant biology that have resulted from microbiome analyses as well as the microbial factors that direct plant fitness and adaptability in natural systems.

  14. Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f.sp. hordei

    DEFF Research Database (Denmark)

    Christiansen, S.K.; Knudsen, S.; Giese, H.

    1995-01-01

    Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces, GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta...

  15. Plant metabolites of the Siberian flora. Chemical transformations and the scope of practical application

    Energy Technology Data Exchange (ETDEWEB)

    Shults, Elvira E; Raldugin, Victor A; Volcho, Konstantin P; Salakhutdinov, Nariman F; Tolstikov, Genrikh A [N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk (Russian Federation)

    2007-07-31

    The results of studies of some terpenoids, alkaloids and phenolic derivatives isolated from Siberian plants are summarised. The structures of the compounds studied are presented and the chemical transformations of the available terpenoids and alkaloids are considered. Examples of practical application of natural compounds and their derivatives are given.

  16. Plant metabolites of the Siberian flora. Chemical transformations and the scope of practical application

    Science.gov (United States)

    Shults, Elvira E.; Raldugin, Victor A.; Volcho, Konstantin P.; Salakhutdinov, Nariman F.; Tolstikov, Genrikh A.

    2007-07-01

    The results of studies of some terpenoids, alkaloids and phenolic derivatives isolated from Siberian plants are summarised. The structures of the compounds studied are presented and the chemical transformations of the available terpenoids and alkaloids are considered. Examples of practical application of natural compounds and their derivatives are given.

  17. Effect of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity and Other Factors in Successful Plant Transformation

    Directory of Open Access Journals (Sweden)

    Sandip S. Magdum

    2013-08-01

    Full Text Available Agrobacterium tumefaciens infection and antibiotic wash are the critical steps of Agrobacterium mediated plant transformation procedure, most time responsible for lower transformation efficiency due to necrosis and phytotoxicity caused by biotic stress of Agrobacterium and abiotic stress by antibiotics respectively. Ammi majus Egyptian origin medicinal plant and Pearl millet cereal grain crop were studied for their stress responses to Agrobacterium mediated transformation (AMT. Agrobacterium strains LBA4404 (O.D.=0.6-0.8 and EHA105 (O.D.=0.2-0.4 were used for transformation experiments to infect calli of Ammi majus and embryogenic calli of Pearl millet respectively. Incase of antibiotic wash, Cefotaxime 500 mg L-1 was used for LBA4404 infected Ammi majus calli and Timentin 300 mg L-1 was used for EHA105 infected embryogenic calli of Pearl millet. Effects of Agrobacterium infection, antibiotic and NaOCl washes on Agrobacterium removal and both explants physiological changes during transformation experimental procedures were studied. At the end of the experiments explants survival efficiency of Ammi majus and pearl millet were 8% and 5% respectively. Biotic and abiotic stress factors responsible for lower efficiency were investigated with various other factors and strategies were discussed which are need to be considered for higher transformation events and target tissue survival.

  18. Time lens based optical fourier transformation for advanced processing of spectrally-efficient OFDM and N-WDM signals

    DEFF Research Database (Denmark)

    Guan, Pengyu; Røge, Kasper Meldgaard; Morioka, Toshio

    2016-01-01

    We review recent progress in the use of time lens based optical Fourier transformation for advanced optical signal processing, with focus on all-optical generation, detection and format conversion of spectrally-efficient OFDM and N-WDM signals.......We review recent progress in the use of time lens based optical Fourier transformation for advanced optical signal processing, with focus on all-optical generation, detection and format conversion of spectrally-efficient OFDM and N-WDM signals....

  19. Defining the needs for gas centrifuge enrichment plants advanced safeguards

    Energy Technology Data Exchange (ETDEWEB)

    Boyer, Brian David [Los Alamos National Laboratory; Erpenbeck, Heather H [Los Alamos National Laboratory; Miller, Karen A [Los Alamos National Laboratory; Swinhoe, Martyn T [Los Alamos National Laboratory; Ianakiev, Kiril [Los Alamos National Laboratory; Marlow, Johnna B [Los Alamos National Laboratory

    2010-04-05

    Current safeguards approaches used by the International Atomic Energy Agency (IAEA) at gas centrifuge enrichment plants (GCEPs) need enhancement in order to verify declared low-enriched (LEU) production, detect undeclared LEU production and detect highly enriched uranium (HEU) production with adequate detection probability using nondestructive assay (NDA) techniques. At present inspectors use attended systems, systems needing the presence of an inspector for operation, during inspections to verify the mass and {sup 235}U enrichment of declared UF{sub 6} containers used in the process of enrichment at GCEPs. In verifying declared LEU production, the inspectors also take samples for off-site destructive assay (DA) which provide accurate data, with 0.1% to 0.5% measurement uncertainty, on the enrichment of the UF{sub 6} feed, tails, and product. However, taking samples of UF{sub 6} for off-site analysis is a much more labor and resource intensive exercise for the operator and inspector. Furthermore, the operator must ship the samples off-site to the IAEA laboratory which delays the timeliness of results and interruptions to the continuity of knowledge (CofK) of the samples during their storage and transit. This paper contains an analysis of possible improvements in unattended and attended NDA systems such as process monitoring and possible on-site analysis of DA samples that could reduce the uncertainty of the inspector's measurements and provide more effective and efficient IAEA GCEPs safeguards. We also introduce examples advanced safeguards systems that could be assembled for unattended operation.

  20. Advances in transforming kudzu (Pueraria phaseoloides and carrot (Daucus carota var. Danvers 126 roots with different Agrobacterium rhizogenes strains for increasing MA fungi growth

    Directory of Open Access Journals (Sweden)

    Marisol Medina Sierra

    2011-12-01

    Full Text Available Kudzú (P. phaseoloides and carrot (D. carota roots were transformed in this survey into different kinds of culture medium by using five different A. rhizogenes strains. These presented different behaviour both in carrot transformation by A. rhizogenes 15834, A.r.8196 and A.r.2659 strains as well as kudzu transformation by A.r.15834 and A.r.1724 strains. Transformed carrot root growth was increased in WM culture medium, whilst transformed kudzu root growth did not increase in either the same medium or in modified MS medium. Transformed carrot roots were used for G. intrarradices increase and sporulation; however, wild AMF strains, isolated from a mining area (the lower Cauca area of Antioquia, did not grow either in roots from this specie or those from kudzu, in spite of this plant having great affinity for wild AMF strains. The results represent an advance in the procedure for DNA isolation and keeping AMF collections, required for other research.

  1. Agrobacterium rhizogenes-mediated transformation of the parasitic plant Phtheirospermum japonicum.

    Directory of Open Access Journals (Sweden)

    Juliane K Ishida

    Full Text Available BACKGROUND: Plants within the Orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. Despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: We developed transient and stable transformation systems for Phtheirospermum japonicum, a facultative parasitic plant in the Orobanchaceae. The transformation protocol was established by a combination of sonication and acetosyringone treatments using the hairy-root-inducing bacterium, Agrobacterium rhizogenes and young seedlings. Transgenic hairy roots of P. japonicum were obtained from cotyledons 2 to 3 weeks after A. rhizogenes inoculation. The presence and the expression of transgenes in P. japonicum were verified by genomic PCR, Southern blot and RT-PCR methods. Transgenic roots derived from A. rhizogenes-mediated transformation were able to develop haustoria on rice and maize roots. Transgenic roots also formed apparently competent haustoria in response to 2,6-dimethoxy-1,4-benzoquinone (DMBQ, a haustorium-inducing chemical. Using this system, we introduced a reporter gene with a Cyclin B1 promoter into P. japonicum, and visualized cell division during haustorium formation. CONCLUSIONS: We provide an easy and efficient method for hairy-root transformation of P. japonicum. Transgenic marker analysis revealed that cell divisions during haustorium development occur 24 h after DMBQ treatment. The protocols described here will allow functional analysis of genes involved in plant parasitism.

  2. Development of an Agrobacterium-mediated stable transformation method for the sensitive plant Mimosa pudica.

    Science.gov (United States)

    Mano, Hiroaki; Fujii, Tomomi; Sumikawa, Naomi; Hiwatashi, Yuji; Hasebe, Mitsuyasu

    2014-01-01

    The sensitive plant Mimosa pudica has long attracted the interest of researchers due to its spectacular leaf movements in response to touch or other external stimuli. Although various aspects of this seismonastic movement have been elucidated by histological, physiological, biochemical, and behavioral approaches, the lack of reverse genetic tools has hampered the investigation of molecular mechanisms involved in these processes. To overcome this obstacle, we developed an efficient genetic transformation method for M. pudica mediated by Agrobacterium tumefaciens (Agrobacterium). We found that the cotyledonary node explant is suitable for Agrobacterium-mediated transformation because of its high frequency of shoot formation, which was most efficiently induced on medium containing 0.5 µg/ml of a synthetic cytokinin, 6-benzylaminopurine (BAP). Transformation efficiency of cotyledonary node cells was improved from almost 0 to 30.8 positive signals arising from the intron-sGFP reporter gene by using Agrobacterium carrying a super-binary vector pSB111 and stabilizing the pH of the co-cultivation medium with 2-(N-morpholino)ethanesulfonic acid (MES) buffer. Furthermore, treatment of the explants with the detergent Silwet L-77 prior to co-cultivation led to a two-fold increase in the number of transformed shoot buds. Rooting of the regenerated shoots was efficiently induced by cultivation on irrigated vermiculite. The entire procedure for generating transgenic plants achieved a transformation frequency of 18.8%, which is comparable to frequencies obtained for other recalcitrant legumes, such as soybean (Glycine max) and pea (Pisum sativum). The transgene was stably integrated into the host genome and was inherited across generations, without affecting the seismonastic or nyctinastic movements of the plants. This transformation method thus provides an effective genetic tool for studying genes involved in M. pudica movements.

  3. Development of an Agrobacterium-mediated stable transformation method for the sensitive plant Mimosa pudica.

    Directory of Open Access Journals (Sweden)

    Hiroaki Mano

    Full Text Available The sensitive plant Mimosa pudica has long attracted the interest of researchers due to its spectacular leaf movements in response to touch or other external stimuli. Although various aspects of this seismonastic movement have been elucidated by histological, physiological, biochemical, and behavioral approaches, the lack of reverse genetic tools has hampered the investigation of molecular mechanisms involved in these processes. To overcome this obstacle, we developed an efficient genetic transformation method for M. pudica mediated by Agrobacterium tumefaciens (Agrobacterium. We found that the cotyledonary node explant is suitable for Agrobacterium-mediated transformation because of its high frequency of shoot formation, which was most efficiently induced on medium containing 0.5 µg/ml of a synthetic cytokinin, 6-benzylaminopurine (BAP. Transformation efficiency of cotyledonary node cells was improved from almost 0 to 30.8 positive signals arising from the intron-sGFP reporter gene by using Agrobacterium carrying a super-binary vector pSB111 and stabilizing the pH of the co-cultivation medium with 2-(N-morpholinoethanesulfonic acid (MES buffer. Furthermore, treatment of the explants with the detergent Silwet L-77 prior to co-cultivation led to a two-fold increase in the number of transformed shoot buds. Rooting of the regenerated shoots was efficiently induced by cultivation on irrigated vermiculite. The entire procedure for generating transgenic plants achieved a transformation frequency of 18.8%, which is comparable to frequencies obtained for other recalcitrant legumes, such as soybean (Glycine max and pea (Pisum sativum. The transgene was stably integrated into the host genome and was inherited across generations, without affecting the seismonastic or nyctinastic movements of the plants. This transformation method thus provides an effective genetic tool for studying genes involved in M. pudica movements.

  4. INFLUENCE OF BIOLOGICAL AND THERMAL TRANSFORMED SEWAGE SLUDGE APPLICATION ON MANGANESE CONTENT IN PLANTS AND SOIL

    Directory of Open Access Journals (Sweden)

    Małgorzata Koncewicz-Baran

    2014-10-01

    Full Text Available A great variety of sewage sludge treatment methods, due to the agent (chemical, biological, thermal leads to the formation of varying ‘products’ properties, including the content of heavy metals forms. The aim of the study was to determine the effects of biologically and thermally transformed sewage sludge on the manganese content in plants and form of this element in the soil. The study was based on a two-year pot experiment. In this study was used stabilized sewage sludge collected from Wastewater Treatment Plant Krakow – ”Płaszów” and its mixtures with wheat straw in the gravimetric ratio 1:1 in conversion to material dry matter, transformed biologically (composting by 117 days in a bioreactor and thermally (in the furnace chamber with no air access by the following procedure exposed to temperatures of 130 °C for 40 min → 200 °C for 30 min. In both years of the study biologically and thermally transformed mixtures of sewage sludge with wheat straw demonstrated similar impact on the amount of biomass plants to the pig manure. Bigger amounts of manganese were assessed in oat biomass than in spring rape biomass. The applied sewage sludge and its biologically and thermally converted mixtures did not significantly affect manganese content in plant biomass in comparison with the farmyard manure. The applied fertilization did not modify the values of translocation and bioaccumulation ratios of manganese in the above-ground parts and roots of spring rape and oat. No increase in the content of the available to plants forms of manganese in the soil after applying biologically and thermally transformed sewage sludge mixtures with straw was detected. In the second year, lower contents of these manganese forms were noted in the soil of all objects compared with the first year of the experiment.

  5. Selenium Content, Influential Factors Within the Plant and the Transformation of Different Selenium Specification

    Directory of Open Access Journals (Sweden)

    LIU Yuan-yuan

    2014-12-01

    Full Text Available The paper collected relevant literatures on selenium and explored the function to plant, selenium content, influential factors and selenium specification and transformation. We believed that there should be more deep researches on function of selenium to plant. Approaches of molecular, genetic engineering and isotope could be employed to breed selenium rich crops and possibilities in practice. More efforts should be spent on the technologies research for improving selenium level in crops under natural soil conditions to sustainably utilize the selenium resources.

  6. Transformation

    DEFF Research Database (Denmark)

    Peters, Terri

    2011-01-01

    Artiklen diskuterer ordet "transformation" med udgangspunkt i dels hvorledes ordet bruges i arkitektfaglig terminologi og dels med fokus på ordets potentielle indhold og egnethed i samme teminologi.......Artiklen diskuterer ordet "transformation" med udgangspunkt i dels hvorledes ordet bruges i arkitektfaglig terminologi og dels med fokus på ordets potentielle indhold og egnethed i samme teminologi....

  7. Transformation

    DEFF Research Database (Denmark)

    Peters, Terri

    2011-01-01

    Artiklen diskuterer ordet "transformation" med udgangspunkt i dels hvorledes ordet bruges i arkitektfaglig terminologi og dels med fokus på ordets potentielle indhold og egnethed i samme teminologi.......Artiklen diskuterer ordet "transformation" med udgangspunkt i dels hvorledes ordet bruges i arkitektfaglig terminologi og dels med fokus på ordets potentielle indhold og egnethed i samme teminologi....

  8. Advances in Melatonin and Its Functions in Plants

    Institute of Scientific and Technical Information of China (English)

    Na ZHANG; Haijun ZHANG; Rongchao YANG; Yunyu HUANG; Yangdong GUO

    2012-01-01

    Abstract Melatonin (N-acetyi-5-methoxytryptamine) is a well-known animal hormone, which is synthesized and secreted by pineal gland and takes part in the regulation of circadian rhythm in animals. At present it has been observed that melatonin is widely existed in higher plants while there have no enough studies on functions of melatonin in plants. Researches have already indicated that the possible functions of melatonin in plants include regulating photoperiod, participating in growth regulation, clearing active oxygen, and promoting activity of antioxidase. General reviews upon functions of melatonin in plants are made upon experiments in recent years. We fo-cus on the demonstrated and predicted biological functions of melatonin in plants to bring researchers up to date on this field. The weakness in present studies and the main research directions are also pointed out.

  9. Nitrogen transformations and retention in planted and artificially aerated constructed wetlands.

    Science.gov (United States)

    Maltais-Landry, Gabriel; Maranger, Roxane; Brisson, Jacques; Chazarenc, Florent

    2009-02-01

    Nitrogen (N) processing in constructed wetlands (CWs) is often variable, and the contribution to N loss and retention by various pathways (nitrification/denitrification, plant uptake and sediment storage) remains unclear. We studied the seasonal variation of the effects of artificial aeration and three different macrophyte species (Phragmites australis, Typha angustifolia and Phalaris arundinacea) on N processing (removal rates, transformations and export) using experimental CW mesocosms. Removal of total nitrogen (TN) was higher in summer and in planted and aerated units, with the highest mean removal in units planted with T. angustifolia. Export of ammonium (NH(4)(+)), a proxy for nitrification limitation, was higher in winter, and in unplanted and non-aerated units. Planted and aerated units had the highest export of oxidized nitrogen (NO(y)), a proxy for reduced denitrification. Redox potential, evapotranspiration (ETP) rates and hydraulic retention times (HRT) were all predictors of TN, NH(4)(+) and NO(y) export, and significantly affected by plants. Denitrification was the main N sink in most treatments accounting for 47-62% of TN removal, while sediment storage was dominant in unplanted non-aerated units and units planted with P. arundinacea. Plant uptake accounted for less than 20% of the removal. Uncertainties about the long-term fate of the N stored in sediments suggest that the fraction attributed to denitrification losses could be underestimated in this study.

  10. An intellectual property sharing initiative in agricultural biotechnology: development of broadly accessible technologies for plant transformation.

    Science.gov (United States)

    Chi-Ham, Cecilia L; Boettiger, Sara; Figueroa-Balderas, Rosa; Bird, Sara; Geoola, Josef N; Zamora, Pablo; Alandete-Saez, Monica; Bennett, Alan B

    2012-06-01

    The Public Intellectual Property Resource for Agriculture (PIPRA) was founded in 2004 by the Rockefeller Foundation in response to concerns that public investments in agricultural biotechnology benefiting developing countries were facing delays, high transaction costs and lack of access to important technologies due to intellectual property right (IPR) issues. From its inception, PIPRA has worked broadly to support a wide range of research in the public sector, in specialty and minor acreage crops as well as crops important to food security in developing countries. In this paper, we review PIPRA's work, discussing the failures, successes, and lessons learned during its years of operation. To address public sector's limited freedom-to-operate, or legal access to third-party rights, in the area of plant transformation, we describe PIPRA's patent 'pool' approach to develop open-access technologies for plant transformation which consolidate patent and tangible property rights in marker-free vector systems. The plant transformation system has been licensed and deployed for both commercial and humanitarian applications in the United States (US) and Africa, respectively.

  11. pORE: a modular binary vector series suited for both monocot and dicot plant transformation.

    Science.gov (United States)

    Coutu, Catherine; Brandle, James; Brown, Dan; Brown, Kirk; Miki, Brian; Simmonds, John; Hegedus, Dwayne D

    2007-12-01

    We present a series of 14 binary vectors suitable for Agrobacterium-mediated transformation of dicotyledonous plants and adaptable for biolistic transformation of monocotyledonous plants. The vector size has been minimized by eliminating all non-essential elements from the vector backbone and T-DNA regions while maintaining the ability to replicate independently. The smallest of the vector series is 6.3 kb and possesses an extensive multiple cloning site with 21 unique restriction endonuclease sites that are compatible with common cloning, protein expression, yeast two-hybrid and other binary vectors. The T-DNA region was engineered using a synthetic designer oligonucleotide resulting in an entirely modular system whereby any vector element can be independently exchanged. The high copy number ColE1 origin of replication has been included to enhance plasmid yield in Escherichia coli. FRT recombination sites flank the selectable marker cassette regions and allow for in planta excision by FLP recombinase. The pORE series consists of three basic types; an 'open' set for general plant transformation, a 'reporter' set for promoter analysis and an 'expression' set for constitutive expression of transgenes. The sets comprise various combinations of promoters (P (HPL), P (ENTCUP2) and P (TAPADH)), selectable markers (nptII and pat) and reporter genes (gusA and smgfp).

  12. ESCRIME: testing bench for advanced operator workstations in future plants

    Energy Technology Data Exchange (ETDEWEB)

    Poujol, A.; Papin, B. [CEA Centre d`Etudes Nucleaires de Cadarache, 13 - Saint-Paul-lez-Durance (France)

    1994-12-31

    The problem of optimal task allocation between man and computer for the operation of nuclear power plants is of major concern for the design of future plants. As the increased level of automation induces the modification of the tasks actually devoted to the operator in the control room, it is very important to anticipate these consequences at the plant design stage. The improvement of man machine cooperation is expected to play a major role in minimizing the impact of human errors on plant safety. The CEA has launched a research program concerning the evolution of the plant operation in order to optimize the efficiency of the human/computer systems for a better safety. The objective of this program is to evaluate different modalities of man-machine share of tasks, in a representative context. It relies strongly upon the development of a specific testing facility, the ESCRIME work bench, which is presented in this paper. It consists of an EDF 1300MWe PWR plant simulator connected to an operator workstation. The plant simulator model presents at a significant level of details the instrumentation and control of the plant and the main connected circuits. The operator interface is based on the generalization of the use of interactive graphic displays, and is intended to be consistent to the tasks to be performed by the operator. The functional architecture of the workstation is modular, so that different cooperation mechanisms can be implemented within the same framework. It is based on a thorough analysis and structuration of plant control tasks, in normal as well as in accident situations. The software architecture design follows the distributed artificial intelligence approach. Cognitive agents cooperate in order to operate the process. The paper presents the basic principles and the functional architecture of the test bed and describes the steps and the present status of the program. (author).

  13. Reference Operational Concepts for Advanced Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Hugo, Jacques Victor [Idaho National Lab. (INL), Idaho Falls, ID (United States); Farris, Ronald Keith [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-09-01

    This report represents the culmination of a four-year research project that was part of the Instrumentation and Control and Human Machine Interface subprogram of the DOE Advanced Reactor Technologies program.

  14. Recent advances in plant-herbivore interactions [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Deron E. Burkepile

    2017-02-01

    Full Text Available Plant-herbivore interactions shape community dynamics across marine, freshwater, and terrestrial habitats. From amphipods to elephants and from algae to trees, plant-herbivore relationships are the crucial link generating animal biomass (and human societies from mere sunlight. These interactions are, thus, pivotal to understanding the ecology and evolution of virtually any ecosystem. Here, we briefly highlight recent advances in four areas of plant-herbivore interactions: (1 plant defense theory, (2 herbivore diversity and ecosystem function, (3 predation risk aversion and herbivory, and (4 how a changing climate impacts plant-herbivore interactions. Recent advances in plant defense theory, for example, highlight how plant life history and defense traits affect and are affected by multiple drivers, including enemy pressure, resource availability, and the local plant neighborhood, resulting in trait-mediated feedback loops linking trophic interactions with ecosystem nutrient dynamics. Similarly, although the positive effect of consumer diversity on ecosystem function has long been recognized, recent advances using DNA barcoding to elucidate diet, and Global Positioning System/remote sensing to determine habitat selection and impact, have shown that herbivore communities are probably even more functionally diverse than currently realized. Moreover, although most diversity-function studies continue to emphasize plant diversity, herbivore diversity may have even stronger impacts on ecosystem multifunctionality. Recent studies also highlight the role of risk in plant-herbivore interactions, and risk-driven trophic cascades have emerged as landscape-scale patterns in a variety of ecosystems. Perhaps not surprisingly, many plant-herbivore interactions are currently being altered by climate change, which affects plant growth rates and resource allocation, expression of chemical defenses, plant phenology, and herbivore metabolism and behavior. Finally

  15. TRANSFORMER

    Science.gov (United States)

    Baker, W.R.

    1959-08-25

    Transformers of a type adapted for use with extreme high power vacuum tubes where current requirements may be of the order of 2,000 to 200,000 amperes are described. The transformer casing has the form of a re-entrant section being extended through an opening in one end of the cylinder to form a coaxial terminal arrangement. A toroidal multi-turn primary winding is disposed within the casing in coaxial relationship therein. In a second embodiment, means are provided for forming the casing as a multi-turn secondary. The transformer is characterized by minimized resistance heating, minimized external magnetic flux, and an economical construction.

  16. [Recent advances in the analysis of gibberellins plant hormones].

    Science.gov (United States)

    Zhang, Xiaona; Lu, Minghua; Xu, Linfang; Xiao, Rui; Cai, Zongwei

    2015-08-01

    Gibberellins (GAs) are a class of phytohormones that exert profound and diverse effects on plant growth and development, such as seed germination and leaf expansion. Up to now, 136 members of GAs have been identified and recognized. All known GAs are diterpenoid acids with similar chemical structures, only double bonds, hydroxyl numbers and locations on gibberellin alkane skeleton are different. However, the content of GAs in plants is of ultra trace levels (usually at ng/g and even pg/g levels) with little ultraviolet (UV) absorption, no fluorescence and no distinguishing chemical characteristics. Moreover, the matrix of plant samples is complicated. Thus, quantification of GAs is always extremely difficult. Nowadays, the bottle necks for the study of GAs in plants are due to the lack of efficient sample preparation and sensitive detection techniques. This article reviews the analytical methods for determination of GAs in recent years, hoping to provide some references to develop new methods and techniques.

  17. Plant genetic resources: Advancing conservation and use through ...

    African Journals Online (AJOL)

    Administrator

    Germplasm Conservation Scientist, International Plant Genetic Resources Institute, Regional Office for Sub-Saharan. Africa, P.O. ... In cacao (Theobroma cocoa), where collecting .... the advent of these new cryogenic procedures, especially.

  18. Advanced technologies for plant cell wall evolution and diversity

    DEFF Research Database (Denmark)

    Fangel, Jonatan Ulrik

    Plant cell walls consist of polysaccharides, glycoproteins and phenolic polymers interlinked together in a highly complex network. The detailed analysis of cell walls is challenging because of their inherent complexity and heterogeneity. Also, complex carbohydrates, unlike proteins and nucleotide...... angiosperms. This analysis has enabled cell wall diversity to be placed in a phylogenetic context, and, when integrated with transcriptomic and genomic analysis has contributed to our understanding of important aspects of plant evolution....

  19. Recent Advances of Flowering Locus T Gene in Higher Plants

    OpenAIRE

    Shuiyuan Cheng; Feng Xu; Xiaofeng Rong; Xiaohua Huang

    2012-01-01

    Flowering Locus T (FT) can promote flowering in the plant photoperiod pathway and also facilitates vernalization flowering pathways and other ways to promote flowering. The expression of products of the FT gene is recognized as important parts of the flowering hormone and can induce flowering by long-distance transportation. In the present study, many FT-like genes were isolated, and the transgenic results show that FT gene can promote flowering in plants. This paper reviews the progress of t...

  20. The dsdA gene from Escherichia coli provides a novel selectable marker for plant transformation.

    Science.gov (United States)

    Erikson, Oskar; Hertzberg, Magnus; Näsholm, Torgny

    2005-02-01

    Plants are sensitive to D-serine, but functional expression of the dsdA gene, encoding D-serine ammonia lyase, from Escherichia coli can alleviate this toxicity. Plants, in contrast to many other organisms, lack the common pathway for oxidative deamination of D-amino acids. This difference in metabolism has major consequences for plant responses to D-amino acids, since several D-amino acids are toxic to plants even at relatively low concentrations. Therefore, introducing an enzyme specific for a phytotoxic D-amino acid should generate a selectable characteristic that can be screened. Here we present the use of the dsdA gene as a selectable marker for transformation of Arabidopsis. D-serine ammonia lyase catalyses the deamination of D-serine into pyruvate, water and ammonium. dsdA transgenic seedlings can be clearly distinguished from wild type, having an unambiguous phenotype immediately following germination when selected on D-serine containing medium. The dsdA marker allows flexibility in application of the selective agent: it can be applied in sterile plates, in foliar sprays or in liquid culture. Selection with D-serine resistance was compared with selection based on kanamycin resistance, and was found to generate similar transformation frequencies but also to be more unambiguous, more rapid and more versatile with respect to the way the selective agent can be supplied.

  1. Metabolic engineering by plastid transformation as a strategy to modulate isoprenoid yield in plants.

    Science.gov (United States)

    Hasunuma, Tomohisa; Kondo, Akihiko; Miyake, Chikahiro

    2010-01-01

    Plants synthesize a large number of isoprenoid compounds that have diverse structures and functions. All isoprenoids are synthesized through consecutive condensation of five-carbon precursors, isopentenyl diphosphate (IPP) and its allyl isomer dimethylallyl diphosphate (DMAPP). With recent success in the cloning of genes that encode the enzymes of isoprenoid biosynthesis, genetic engineering strategies for the improvement of plant isoprenoid metabolism have emerged. Plastid transformation technology offers attractive features in plant genetic engineering. It has many advantages over nuclear genome transformation: high-level foreign protein expression, no need for a transit peptide, absence of gene silencing, and convenient transgene stacking in operons. We demonstrated that this technology is a remarkable tool for the production of isoprenoids in plants through metabolic engineering. The expression of bacterial genes encoding CrtW (beta-carotene ketolase) and CrtZ (beta-carotene hydroxylase) or cyanobacterial genes encoding DXR (1-deoxy-D-xylulose-5-phosphate reductoisomerase) in the plastid genome leads to alteration in isoprenoid content of tobacco leaves.

  2. Reproduction of Meloidogyne javanica on Plant Roots Genetically Transformed by Agrobacterium rhizogenes.

    Science.gov (United States)

    Verdejo, S; Jaffee, B A; Mankau, R

    1988-10-01

    Reproduction of Meloidogyne javanica was compared on several Agrobacterium rhizogenes-transformed root cultures under monoxenic conditions. M. javanica reproduced on all transformed roots tested; however, more females and eggs were obtained on potato and South Australian Early Dwarf Red tomato than on bindweed, Tropic tomato, lima bean, or carrot. Roots that grew at moderate rates into the agar and produced many secondary roots supported the highest reproduction. Numbers of females produced in cultures of transformed potato roots increased with increasing nematode inoculum levels, whether inoculum was dispersed eggs or juveniles. Females appeared smaller, produced fewer eggs, and were found in coalesced galls at the higher inoculum levels. The ratio between the final and initial population decreased sharply as the juvenile inoculum increased. The second-stage juvenile was preferred to dispersed eggs or egg masses for inoculation of tissue culture systems because quantity and viability of inoculum were easily assessed. Meloidogyne javanica reared on transformed root cultures were able to complete their life cycles on new transformed root cultures or greenhouse tomato plants.

  3. Aging Management Guideline for commercial nuclear power plants: Power and distribution transformers

    Energy Technology Data Exchange (ETDEWEB)

    Toman, G.; Gazdzinski, R. [Sandia National Labs., Albuquerque, NM (United States)

    1994-05-01

    This Aging Management Guideline (AMG) provides recommended methods for effective detection and mitigation of age-related degradation mechanisms in power and distribution transformers important to license renewal in commercial nuclear power plants. The intent of this AMG to assist plant maintenance and operations personnel in maximizing the safe, useful life of these components. It also supports the documentation of effective aging management programs required under the License Renewal Rule 10 CFR Part 54. This AMG is presented in a manner which allows personnel responsible for performance analysis and maintenance to compare their plant-specific aging mechanisms (expected or already experienced) and aging management program activities to the more generic results and recommendations presented herein.

  4. Arsenic transformations in the soil-rhizosphere-plant system: fundamentals and potential application to phytoremediation.

    Science.gov (United States)

    Fitz, Walter J; Wenzel, Walter W

    2002-11-13

    This paper reviews major processes that potentially affect the fate of arsenic in the rhizosphere of plants. Rhizosphere interactions are deemed to play a key role in controlling bioavailability to crop plants and for a better understanding and improvement of phytoremediation technologies. Substantial progress has been made towards an understanding of As transformation processes in soils. However, virtually no information is available that directly addresses the fate of As in the rhizosphere. We are proposing a conceptual model of the fate of As in the soil-rhizosphere-plant system by integrating the state-of-the art knowledge available in the contributing disciplines. Using this model and recent studies on hyperaccumulation of As, we discuss research needs and the potential application of rhizosphere processes to the development of phytoremediation technologies for As-polluted soils.

  5. Transformation of japonica rice with RHL gene and salt tolerance of the transgenic rice plant

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Overexpression of the yeast HAL2 gene increases salt tolerance of yeast and plant. Rice HAL2-like (RHL) gene was introduced into a japonica rice cultivar HJ19 with Agrobacterium tumefaciens-mediated transformation. Transgenic plants in R0 generation were selected on the principle of GUS-positive, RHL gene PCR-positive and normal growth. Hygromycin-resistant plants of some transgenic lines in R1 generation increased salt tolerance during the seedling and booting stage, being less damaged in the cytomembrane and stronger in leaf tissue viability under salt stress during booting period. Southern analysis of transgenic lines tolerant to salt in R1 generation showed that the RHL gene expression cassette had been successfully integrated into rice genome. Moreover, gene engineering breeding methodology and really salt-tolerant rice cultivar were discussed.

  6. Transformation Laplacian metamaterials: recent advances in manipulating thermal and dc fields

    Science.gov (United States)

    Han, Tiancheng; Qiu, Cheng-Wei

    2016-04-01

    The full control of single or even multiple physical fields has attracted intensive research attention in the past decade, thanks to the development of metamaterials and transformation optics. Significant progress has been made in vector fields (e.g., optics, electromagnetics, and acoustics), leading to a host of strikingly functional metamaterials, such as invisibility cloaks, illusion devices, concentrators, and rotators. However, metamaterials in vector fields, designed through coordinate transformation of Maxwell’s equations, usually require extreme parameters and impose challenges on the actual realization. In this context, metamaterials in scalar fields (e.g., thermal and dc fields), which are mostly governed by the Laplace equation, lead to more plausible and facile implementations, since there are native insulators and excellent conductors (serving as two extreme cases). This paper therefore is particularly dedicated to reviewing the most recent advances in Laplacian metamaterials in manipulating thermal (both transient and steady states) and dc fields, separately and (or) simultaneously. We focus on the theory, design, and realization of thermal/dc functional metamaterials that can be used to control heat flux and electric current at will. We also provide an outlook toward the challenges and future directions in this fascinating area.

  7. Nanometric Scale Investigation of Phase Transformations in Advanced Steels for Automotive Application

    Science.gov (United States)

    Drillet, Josée; Valle, Nathalie; Iung, Thierry

    2012-12-01

    The current trend toward producing lighter vehicles in the automotive industry is driven by the need to conform to the new exhaust emission control regulations. This objective presents a challenge to steel manufacturers. The difficulty lies in designing new alloys with an optimum strength/formability/cost balance for the various components. Here, the key to success lies in controlling the steel microstructure and especially the phase transformations at the smallest possible scale. Among the different alloying elements, light elements such as carbon and boron are of prime importance due to their major effects on the kinetics of phase transformations. Characterization tools combining high spatial and analytical resolution such as secondary ion mass spectrometry (SIMS) and field emission gun-transmission electron microscopy (TEM) were used. In this article, the examples presented are as follows. (1) Boron segregation and precipitation effects to control hardenability in martensitic steels. (2) Local carbon distribution in advanced high-strength steels, with a specific emphasis on martensite tempering. Links have been established between the boron and carbon distribution and the formability.

  8. Advancements in transformation optics-enabled gradient-index lens design

    Science.gov (United States)

    Campbell, Sawyer D.; Brocker, Donovan E.; Nagar, Jogender; Easum, John A.; Werner, Douglas H.; Werner, Pingjuan L.

    2015-09-01

    Transformation Optics (TO) provides the mathematical framework for representing the behavior of electromagnetic radiation in a given geometry by "transforming" it to an alternative, usually more desirable, geometry through an appropriate mapping of the constituent material parameters. Using a quasi-conformal mapping, the restrictions on the required material parameters can be relaxed allowing isotropic inhomogeneous all-dielectric materials to be employed. This approach has led to the development of a new and powerful design tool for gradient-index (GRIN) optical systems. Using TO, aspherical lenses can be transformed to simpler spherical and flat geometries or even rotationally-asymmetric shapes which result in true 3D GRIN profiles. TO can also potentially be extended to collapse an entire lens system into a representative GRIN profile thus reducing its physical dimensions while retaining the optical performance of the original system. However, dispersion effects of the constituent materials often limit the bandwidth of metamaterial and TO structures thus restricting their potential applicability. Nonetheless, with the proper pairing of GRIN profile and lens geometry to a given material system, chromatic aberrations can be minimized. To aid in the GRIN construction, we employ advanced multi-objective optimization algorithms which allow the designer to explicitly view the trade-offs between all design objectives such as RMS spot size, field-of-view (FOV), lens thickness, 𝛥𝑛, and focal drift due to chromatic aberrations. We present an overview of our TO-enabled GRIN lens design process and analysis techniques while demonstrating designs which minimize the presence of mono- and poly-chromatic aberrations and discuss their requisite material systems.

  9. The use of comet assay in plant toxicology: recent advances

    Directory of Open Access Journals (Sweden)

    Conceição LV Santos

    2015-06-01

    Full Text Available The systematic study of genotoxicity in plants induced by contaminants and other stress agents has been hindered to date by the lack of reliable and robust biomarkers. The comet assay is a versatile and sensitive method for the evaluation of DNA damages and DNA repair capacity at single-cell level. Due to its simplicity and sensitivity, and the small number of cells required to obtain robust results, the use of plant comet assay has drastically increased in the last decade. For years its use was restricted to a few model species, e.g. Allium cepa, Nicotiana tabacum, Vicia faba, or Arabidopsis thaliana but this number largely increased in the last years. Plant comet assay has been used to study the genotoxic impact of radiation, chemicals including pesticides, phytocompounds, heavy metals, nanoparticles or contaminated complex matrices. Here we will review the most recent data on the use of this technique as a standard approach for studying the genotoxic effects of different stress conditions on plants. Also, we will discuss the integration of information provided by the comet assay with other DNA-damage indicators, and with cellular responses including oxidative stress, cell division or cell death. Finally, we will focus on putative relations between transcripts related with DNA damage pathways, DNA replication and repair, oxidative stress and cell cycle progression that have been identified in plant cells with comet assays demonstrating DNA damage.

  10. Estimating the number of integrations in transformed plants by quantitative real-time PCR

    Directory of Open Access Journals (Sweden)

    Vaira Anna Maria

    2002-10-01

    Full Text Available Abstract Background When generating transformed plants, a first step in their characterization is to obtain, for each new line, an estimate of how many copies of the transgene have been integrated in the plant genome because this can deeply influence the level of transgene expression and the ease of stabilizing expression in following generations. This task is normally achieved by Southern analysis, a procedure that requires relatively large amounts of plant material and is both costly and labour-intensive. Moreover, in the presence of rearranged copies the estimates are not correct. New approaches to the problem could be of great help for plant biotechnologists. Results By using a quantitative real-time PCR method that requires limited preliminary optimisation steps, we achieved statistically significant estimates of 1, 2 and 3 copies of a transgene in the primary transformants. Furthermore, by estimating the copy number of both the gene of interest and the selectable marker gene, we show that rearrangements of the T-DNA are not the exception, and probably happen more often than usually recognised. Conclusions We have developed a rapid and reliable method to estimate the number of integrated copies following genetic transformation. Unlike other similar procedures, this method is not dependent on identical amplification efficiency between the PCR systems used and does not need preliminary information on a calibrator. Its flexibility makes it appropriate in those situations where an accurate optimisation of all reaction components is impossible or impractical. Finally, the quality of the information produced is higher than what can be obtained by Southern blot analysis.

  11. Waste-to-energy advanced cycles and new design concepts for efficient power plants

    CERN Document Server

    Branchini, Lisa

    2015-01-01

    This book provides an overview of state-of-the-art technologies for energy conversion from waste, as well as a much-needed guide to new and advanced strategies to increase Waste-to-Energy (WTE) plant efficiency. Beginning with an overview of municipal solid waste production and disposal, basic concepts related to Waste-To-Energy conversion processes are described, highlighting the most relevant aspects impacting the thermodynamic efficiency of WTE power plants. The pervasive influences of main steam cycle parameters and plant configurations on WTE efficiency are detailed and quantified. Advanc

  12. Advanced targeted monitoring of high temperature components in power plants

    Energy Technology Data Exchange (ETDEWEB)

    Roos, E.; Maile, K.; Jovanovic, A. [MPA Stuttgart (Germany)

    1998-12-31

    The article presents the idea of targeted monitoring of high-temperature pressurized components in fossil-fueled power plants, implemented within a modular software system and using, in addition to pressure and temperature data, also displacement and strain measurement data. The concept has been implemented as a part of a more complex company-oriented Internet/Intranet system of MPA Stuttgart (ALIAS). ALIAS enables to combine smoothly the monitoring results with those of the off-line analysis, e. g. sensitivity analyses, comparison with preceding experience (case studies), literature search, search in material databases -(experimental and standard data), nonlinear FE-analysis, etc. The concept and the system have been implemented in real plant conditions several power plants in Germany and Europe: one of these applications and its results are described more in detail in the presentation. (orig.) 9 refs.

  13. Use of simulators for validation of advanced plant monitoring systems

    Energy Technology Data Exchange (ETDEWEB)

    Uytterhoeven, G.; Vlaminck, M. De [Belgatom, Brussels (Belgium); Javaux, D. [Cognitive Ergonomics Work-Psychology Department, University of Liege, Sart-Tilman (Belgium)

    1999-07-01

    This paper describes how the full-scope nuclear power plant simulator of Doel (Belgium) was used to assess Situation Awareness for the validation of a process monitoring and supervision system, named DIMOS. The method (derived from a method originally developed for the aerospace industry) has been adapted and applied to compare the efficiency of two versions of the monitoring system: Alarm-masking and non alarm-masking versions of DIMOS have been analysed in their ability to support Situation Awareness, to improve performance and to fulfil the satisfaction of operators. Both normal power plant operating conditions and abnormal operating conditions were simulated and a large number of power plant operators were involved in the evaluation. The paper focuses on the rationale behind the 'Situation Awareness' evaluation, the experiment environment and the results regarding the added value of the alarm masking version of the monitoring system. (author)

  14. Advances in Effects of Sound Waves on Plants

    Institute of Scientific and Technical Information of China (English)

    Reda H. E. Hassanien; HOU Tian-zhen; LI Yu-feng; and LI Bao-ming

    2014-01-01

    Sound waves technology has been applied to different plants. It has been found that sound waves were at different frequencies, sound pressure levels (SPLs), exposure periods, and distances from the source of sound inlfuence plant growth. Experiments have been conducted in the open ifeld and under greenhouse growing conditions with different levels of audible sound frequencies and sound pressure levels. Sound waves at 1 kHz and 100 dB for 1 h within a distance of 0.20 m could signiifcantly promote the division and cell wall lfuidity of callus cells and also signiifcantly enhance the activity of protective enzymes and endogenous hormones. Sound waves stimulation could increase the plant plasma-membrane H+-ATPase activity, the contents of soluble sugar, soluble protein, and amylase activity of callus. Moreover, sound waves could increase the content of RNA and the level of transcription. Stress-induced genes could switch on under sound stimulation. Sound waves at 0.1-1 kHz and SPL of (70±5) dB for 3 h from plant acoustic frequency technology (PAFT) generator within a distance ranged from 30 to 60 m every other day signiifcantly increased the yieldof sweet pepper, cucumber and tomato by 30.05, 37.1 and 13.2%, respectively. Furthermore, the yield of lettuce, spinach, cotton, rice, and wheat were increased by 19.6, 22.7, 11.4, 5.7, and 17.0%, respectively. Sound waves may also strengthen plant immune systems. It has been proved that spider mite, aphids, gray mold, late blight and virus disease of tomatoes in the greenhouses decreased by 6.0, 8.0, 9.0, 11.0, and 8.0%, respectively, and the sheath blight of rice was reduced by 50%. This paper provides an overview of literature for the effects of sound waves on various growth parameters of plant at different growth stages.

  15. Folates in plants: research advances and progress in crop biofortification

    Science.gov (United States)

    Gorelova, Vera; Ambach, Lars; Rébeillé, Fabrice; Stove, Christophe; Van Der Straeten, Dominique

    2017-03-01

    Folates, also known as B9 vitamins, serve as donors and acceptors in one-carbon (C1) transfer reactions. The latter are involved in synthesis of many important biomolecules, such as amino acids, nucleic acids and vitamin B5. Folates also play a central role in the methyl cycle that provides one-carbon groups for methylation reactions. The important functions fulfilled by folates make them essential in all living organisms. Plants, being able to synthesize folates de novo, serve as an excellent dietary source of folates for animals that lack the respective biosynthetic pathway. Unfortunately, the most important staple crops such as rice, potato and maize are rather poor sources of folates. Insufficient folate consumption is known to cause severe developmental disorders in humans. Two approaches are employed to fight folate deficiency: pharmacological supplementation in the form of folate pills and biofortification of staple crops. As the former approach is considered rather costly for the major part of the world population, biofortification of staple crops is viewed as a decent alternative in the struggle against folate deficiency. Therefore strategies, challenges and recent progress of folate enhancement in plants will be addressed in this review. Apart from the ever-growing need for the enhancement of nutritional quality of crops, the world population faces climate change catastrophes or environmental stresses, such as elevated temperatures, drought, salinity that severely affect growth and productivity of crops. Due to immense diversity of their biochemical functions, folates take part in virtually every aspect of plant physiology. Any disturbance to the plant folate metabolism leads to severe growth inhibition and, as a consequence, to a lower productivity. Whereas today’s knowledge of folate biochemistry can be considered very profound, evidence on the physiological roles of folates in plants only starts to emerge. In the current review we will discuss the

  16. Advanced maintenance, inspection & repair technology for nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Hinton, B.M.

    1994-12-31

    Maintenance, inspection, and repair technology for nuclear power plants is outlined. The following topics are discussed: technology for reactor systems, reactor refueling bridge, fuel inspection system, fuel shuffling software, fuel reconstitution, CEA/RCCA/CRA inspection, vessel inspection capabilities, CRDM inspection and repair, reactor internals inspection and repair, stud tensioning system, stud/nut cleaning system, EDM machining technology, MI Cable systems, core exit T/C nozzle assemblies, technology for steam generators, genesis manipulator systems, ECT, UT penetrant inspections, steam generator repair and cleaning systems, technology for balance of plant, heat exchangers, piping and weld inspections, and turbogenerators.

  17. Recent advances of flowering locus T gene in higher plants.

    Science.gov (United States)

    Xu, Feng; Rong, Xiaofeng; Huang, Xiaohua; Cheng, Shuiyuan

    2012-01-01

    Flowering Locus T (FT) can promote flowering in the plant photoperiod pathway and also facilitates vernalization flowering pathways and other ways to promote flowering. The expression of products of the FT gene is recognized as important parts of the flowering hormone and can induce flowering by long-distance transportation. In the present study, many FT-like genes were isolated, and the transgenic results show that FT gene can promote flowering in plants. This paper reviews the progress of the FT gene and its expression products to provide meaningful information for further studies of the functions of FT genes.

  18. Reacting flow simulations of supercritical water oxidation of PCB-contaminated transformer oil in a pilot plant reactor

    Directory of Open Access Journals (Sweden)

    V. Marulanda

    2011-06-01

    Full Text Available The scale-up of a supercritical water oxidation process, based on recent advancements in kinetic aspects, reactor configuration and optimal operational conditions, depends on the research and development of simulation tools, which allow the designer not only to understand the complex multiphysics phenomena that describe the system, but also to optimize the operational parameters to attain the best profit for the process and guarantee its safe operation. Accordingly, this paper reports a multiphysics simulation with the CFD software Comsol Multiphysics 3.3 of a pilot plant reactor for the supercritical water oxidation of a heavily PCB-contaminated mineral transformer oil. The proposed model was based on available information for the kinetic aspects of the complex mixture and the optimal operational conditions obtained in a lab-scale continuous supercritical water oxidation unit. The pilot plant simulation results indicate that it is not feasible to scale-up directly the optimal operational conditions obtained in the isothermal lab-scale experiments, due to the excess heat released by the exothermic oxidation reactions that result in outlet temperatures higher than 600°C, even at reactor inlet temperatures as low as 400°C. Consequently, different alternatives such as decreasing organic flowrates or a new reactor set-up with multiple oxidant injections should be considered to guarantee a safe operation.

  19. [Study on transformation of snowdrop lectin gene to chrysanthemum and aphid resistance of the transgenic plants].

    Science.gov (United States)

    Wang, Guan-Lin; Liu, Yan-Hong; Guo, Shao-Hua; Wang, Yu; Ji, Yan; Fang, Hong-Jun

    2004-12-01

    Agrobacterium-mediated transformation in chrysanthemum was studied to prevent the insect pest of aphid (Mizus persicae). The gna gene was successfully transferred into chrysanthemum by leaf dish, and 93 transgenic clones were obtained. The highest transformation frequency 11.21% was achieved on the optimization facts, which were medium YEB with pH5.6, bacterial concentration OD600 = 0.4, precultivation for one day, cocultivation for four days, the cocultivation media supplemented with GA3 0.5 mg/L and leaf explants growed for 45 days. The results from PCR and FQ-PCR analysis confirmed that gna gene was integrated into the genome of chrysanthemum plants. The insect bioassay with aphid showed that the aphid resistance of different transgenic plants was difference, and the rate of aphid population inhibition of them were from 10% to 84% with an average rate of 39.4%. The leaf-extracts from different transgenic plants showed varying actinties in red-blood cell bioassay.

  20. Transformation of maize embryogenic calli mediated by ultrasonication and regeneration of fertile transgenic plants

    Institute of Scientific and Technical Information of China (English)

    张宏; 王国英; 谢友菊; 戴景瑞; 许宁; 赵南明; 李太源; 田颖川; 乔利亚; 莽克强

    1997-01-01

    An insecticidal protein gene isolated from Bacillus thuringiensis was transferred into maize by using ultrasonication. The fertile transgenic plants and their progeny were obtained. The Southern hybridization results indi-cated that the foreign gene had integrated into the maize genome. It has been found that the acoustic intensity and the duration of treatment are the important parameters influencing transformation efficiency by ultrasonication. The maxi-mum relative transformation frequency of 34. 1 % was achieved after 30 min of sonication at 0. 5 W/cm2 acoustic inten-sity. With appropriate parameters the ultrasonication ran make a number of micropores formed on the cell surface and minimize the treatment damage to the foreign DNA molecules, thus facilitating the DNA molecules to enter the cells.

  1. Technical analysis of advanced wastewater-treatment systems for coal-gasification plants

    Energy Technology Data Exchange (ETDEWEB)

    1981-03-31

    This analysis of advanced wastewater treatment systems for coal gasification plants highlights the three coal gasification demonstration plants proposed by the US Department of Energy: The Memphis Light, Gas and Water Division Industrial Fuel Gas Demonstration Plant, the Illinois Coal Gasification Group Pipeline Gas Demonstration Plant, and the CONOCO Pipeline Gas Demonstration Plant. Technical risks exist for coal gasification wastewater treatment systems, in general, and for the three DOE demonstration plants (as designed), in particular, because of key data gaps. The quantities and compositions of coal gasification wastewaters are not well known; the treatability of coal gasification wastewaters by various technologies has not been adequately studied; the dynamic interactions of sequential wastewater treatment processes and upstream wastewater sources has not been tested at demonstration scale. This report identifies key data gaps and recommends that demonstration-size and commercial-size plants be used for coal gasification wastewater treatment data base development. While certain advanced treatment technologies can benefit from additional bench-scale studies, bench-scale and pilot plant scale operations are not representative of commercial-size facility operation. It is recommended that coal gasification demonstration plants, and other commercial-size facilities that generate similar wastewaters, be used to test advanced wastewater treatment technologies during operation by using sidestreams or collected wastewater samples in addition to the plant's own primary treatment system. Advanced wastewater treatment processes are needed to degrade refractory organics and to concentrate and remove dissolved solids to allow for wastewater reuse. Further study of reverse osmosis, evaporation, electrodialysis, ozonation, activated carbon, and ultrafiltration should take place at bench-scale.

  2. Advanced technologies for plant cell wall evolution and diversity

    DEFF Research Database (Denmark)

    Fangel, Jonatan Ulrik

    Plant cell walls consist of polysaccharides, glycoproteins and phenolic polymers interlinked together in a highly complex network. The detailed analysis of cell walls is challenging because of their inherent complexity and heterogeneity. Also, complex carbohydrates, unlike proteins and nucleotides...... probes (monoclonal antibodies mAbs and carbohydrate binding modules, CBMs) to rapidly profile polysaccharides across a sample set. During my PhD I have further developed the CoMPP technique and used it for cell wall analysis within the context of a variety of applied and fundamental projects. The data...... produced has provided new insight into cell wall evolution and biosynthesis and has contributed to the commercial development of cell wall materials. A major focus of the work has been the wide scale sampling of cell wall diversity across the plant kingdom, from unicellular algae to highly evolved...

  3. System configuration for advanced water management in power plants

    Energy Technology Data Exchange (ETDEWEB)

    Queirazza, G.; Sigon, F.; Zagano, C. [Ente Nazionale per l`Energia Elettrica, Milan (Italy)

    1995-12-01

    Water ie required for power plant operation and electricity generation. The water demand is steadily increasing depending on the enrgy pro-capite demand, the available or innovative technologies for power generation and the need for emissions control. Water management is also required to comply with the regulatory trends and it agrees with the guidelines for the sustainable development, as recommended at the Rio conference (Agenda 21). In order to assess the design and the operating alternatives for the water system of power plants and the impact of innovative technologies, a simulation code has been developed. The ENEL proprietary WATERSOFT code is presented in this paper. Some significant results will be presented and discussed, within the frame of improving the water management and optimizing the overall performances of the actual water systems.

  4. Antioxidant potential of Agrobacterium-transformed and non-transformed Physalis ixocarpa plants grown in vitro and ex vitro 

    Directory of Open Access Journals (Sweden)

    Katarzyna Bergier

    2012-12-01

    Full Text Available Introduction:Oxidative stress is involved in pathogenesis of a number of chronic diseases hence is an increasing interest in plant-derived natural antioxidants with respect to their potential health benefits. Plants from the genus Physalis are particularly rich in secondary metabolites and show significant antioxidant potential. Recent development in transgenic research has opened new possibilities for enhanced production of secondary metabolites with plant cell and organ cultures. The hairy root-regenerated Physalis ixocarpa plants grown in vitro and ex vitro were compared to the non-transformed plants with respect to their antioxidant potential.Material/Methods:The total antioxidant capacity (TAC, the contents of total phenols and ascorbate were evaluated in fruits, flowers, leaves and roots of P. ixocarpa using the ferric reducing antioxidant power assay (FRAP, the Folin-Ciocalteu method and the 2,2’-dipyridyl method, respectively.Results/Discussion:The antioxidant profiles, in terms of TAC, ascorbate and phenols were organ-specific and depended on the culture conditions. Neither the total phenol content nor the ascorbate level appeared to determine the TAC of the studied plant extracts. The aqueous extracts exhibited lower antioxidant activities than the acetone ones indicating that lipophilic antioxidants made a major contribution to TAC of the plant tissues. Agrobacterium rhizogenes-mediated transformation changed the antioxidant status with respect to TAC, phenols and ascorbate and this effect was observed in the plants grown in vitro and ex vitro. 

  5. Current advances of DNA barcoding study in plants

    OpenAIRE

    Shuping Ning; Haifei Yan; Gang Hao; Xuejun Ge

    2008-01-01

    DNA barcoding has become one of hotspots of biodiversity research in the last five years. It is a method of rapid and accurate species identification and recognition using a short, standardized DNA region. DNA barcoding is now well established for animals, using a portion of the mitochondrial cytochrome c oxidase subunit 1 (COI or cox1) as the standard universal barcode. However, in plants, progress has been hampered by slow substitution rates in mitochondrial DNA. A number of different chlor...

  6. Advances in plant proteomics-key techniques of proteome

    Institute of Scientific and Technical Information of China (English)

    Songlin RUAN; Huasheng MA; Shiheng WANG; Ya XIN; Lihua QIAN; Jianxing TONG; Jie WANG

    2008-01-01

    Following the completion of genome sequen-cing of model plants,such as rice (Oryza sativa L.) and Arabidopsis thaliana,the era of functional plant genomics has arrived which provides a solid basis for the develop-ment of plant proteomics.We review the background and concepts of proteomics,as well as the key techniques which include:(1) separation techniques such as 2-DE (two-dimensional electrophoresis),RP-HPLC (reverse phase high performance liquid chromatography) and SELDI (surface enhanced laser desorption/ionization) protein chip; (2) mass spectrometry such as MALDI-TOF-MS (matrix assisted laser desorption/ionization-time of flight- mass spectrometry) and ESI-MS/MS (elec-trospray ionization mass spectrometry/mass spectro-metry); (3) Peptide sequence tags; (4) databases related to proteomics; (5) quantitative proteome; (6) TAP (tandem affinity purification) and (7) yeast two-hybrid system.In addition,the challenges and prospects of pro-teomics are also discussed.

  7. [Research advance in seed germination of desert woody plants].

    Science.gov (United States)

    Chang, Wei; Wu, Jian-guo; Liu, Yan-hong

    2007-02-01

    This paper reviewed the research methods of desert woody plants seed germination, and the effects of internal and external ecological factors on it. Most researchers use incubator and artificial climate chamber to dispose the seeds, while field investigation was few involved. Seed dormancy is the important physiological factor affecting germination, while seed size, mass and color are closely correlated with its maturity and vigor. The poor permeability of seed capsule is a barrier that restrains the germination, which can be weakened or eliminated by shaving, cutting, treating with low temperature, and dipping in chemical reagent, etc. Seed water content has a close correlation with its storage life and water-absorbing capability. Suitable temperature is the prerequisite of seed germination, while changing temperature can accelerate the germination. Soil moisture content is a limiting factor, while illumination is not so essential to the seed germination of most desert woody plants. Sand-burying plays an important role in the seed germination through regulating illumination, temperature, and soil moisture content. Salinity stress restrains the seed germination of desert woody plants observably. In further studies, the effects of multi-factors and the eco-physiological and molecular biological mechanisms of germination should be more concerned.

  8. Transformation of polyfluorinated compounds in natural waters by advanced oxidation processes.

    Science.gov (United States)

    Anumol, Tarun; Dagnino, Sonia; Vandervort, Darcy R; Snyder, Shane A

    2016-02-01

    The presence of perfluorocarboxylic acids (PFCAs) in source and finished drinking waters is a concern with studies showing bioaccumulation and adverse toxicological effects in wildlife and potentially humans. Per/Polyfluoroalkyl substances (PFAS) such as fluorotelomer alcohols have been identified as precursors for PFCAs in biological pathways. In this study, we investigated the fate of 6:2 and 8:2 homologues of the fluorotelomer unsaturated carboxylic acids (FTUCAs) during advanced oxidation process (AOPs). Results showed 6:2 FTUCA and 8:2 FTUCA transformed into 6-C PFCA (PFHxA) and 8-C PFCA (PFOA) respectively with very little other PFCA formation for all AOPs. The degradation of 6:2 FTUCA and 8:2 FTUCA was greater in the GW compared to SW for the ozone processes but similar for UV/H2O2. The formation of n-C PFCA followed O3>O3/H2O2 at same dose and UV/H2O2 had much lower formation at the doses tested. Non-targeted analysis with the LC-MS-qTOF indicated the production of other PFCAs which contribute to the total mass balance, although no intermediate product was discovered indicating a rapid and direct transformation from the FTUCAs to the PFCAs and/or significant volatilization of intermediates. With the use of AOPs essential to water reuse treatment schemes, this work raises concerns over the risk of potential formation of PFCAs in the treatment and their adverse health effects in finished drinking water.

  9. Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene

    Institute of Scientific and Technical Information of China (English)

    金松恒; 蒋德安; 李雪芹; 孙骏威

    2004-01-01

    Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased sig-nificantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types,although they showed 70% lower rate of photosynthesis, whichRubsico activase and/or the reduction in carbamylation.was likely an acclimation response to the reduction in Rubsico activase and/or the reduction in carbamylation.

  10. Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene

    Institute of Scientific and Technical Information of China (English)

    金松恒; 蒋德安; 李雪芹; 孙骏威

    2004-01-01

    Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased significantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types,although they showed 70% lower rate of photosynthesis, which was likely an acclimation response to the reduction inRubsico activase and/or the reduction in carbamylation.

  11. Effect of Weak Light on the Photoassimilates Distribution and Transformation of Young Grape Plants

    Institute of Scientific and Technical Information of China (English)

    ZHAN Ji-cheng; HUANG Wei-dong; WANG Li-jun

    2002-01-01

    The effect of weak light on the photoassimilates distribution and transformation of young Jingyu grape plants ( Vitis vinefera L. cv. Jingyn) were studied by shading. Compared to the grape grown under natural light intensity environment, the net photosynthetic rate diurnal variation curve of experimental plants grown in weak light intensity environment was remarkably lower. Its leaf and stem biomass ratio increased when light intensity decreased, the root and new shoot biomass ratio showed an opposite trend. The 14C-photoassimilates was mostly distributed to young leaves and stem, a little was distributed to root. The metabolism of 14C-photoassimilates distributed to the entire grape body were also changed under weak light intensity environment.

  12. Characteristics of photosynthesis in rice plants transformed with an antisense Rubisco activase gene.

    Science.gov (United States)

    Jin, Song-Heng; Jiang, De-An; Li, Xue-Qin; Sun, Jun-Wei

    2004-08-01

    Transgenic rice plants with an antisense gene inserted via Agrobacterium tumefaciens were used to explore the impact of the reduction of Rubisco activase (RCA) on Rubisco and photosynthesis. In this study, transformants containing 15% to 35% wild type Rubisco activase were selected, which could survive in ambient CO2 concentration but grew slowly compared with wild type controls. Gas exchange measurements indicated that the rate of photosynthesis decreased significantly, while stomatal conductance and transpiration rate did not change; and that the intercellular CO2 concentration even increased. Rubisco determination showed that these plants had approximately twice as much Rubisco as the wild types, although they showed 70% lower rate of photosynthesis, which was likely an acclimation response to the reduction in Rubsico activase and/or the reduction in carbamylation.

  13. Evaluation of Nicotiana tabacum plants transformed for the expression of verocytotoxic Escherichia coli antigens.

    Directory of Open Access Journals (Sweden)

    Angela Lombardi

    2015-07-01

    Full Text Available Two transgenic Nicotiana tabacum plants, carrying respectively the F18 adhesive fimbriae and the B subunit of verocytotoxin genes from O138 Verocytotoxic E.coli serotype were developed as a model of edible vaccine. Tobacco plants were transformed by agroinfection according to Rossi et al. (2013 stably.  The F18 adhesive fimbriae and VT2e B-subunit were placed under control of the GLOB promoter for the seed-specific protein expression. Agrobacterium tumefaciens binary vector system is an efficient tool to transform plant cells; however, the exogenous gene integrates at semi-random into the nuclear chromosome. PCR products, using specific oligonucleotides putatively encoding the B-subunit of VT2e-B and F18 fimbriae were identified on agarose gel (1.5% - 0.9% as bands with a length of 270 and 519 base pairs, respectively. We showed that the foreign VT2e-B and F18 fimbriae genes were stably integrated into the tobacco genome. Northern blot and Western blot analyses carried out respectively on total mRNA and total soluble protein extract obtained from seeds. For each line, the obtained amount of antigens is sufficient for subsequent oral immunization trials. Three lines of tobacco seeds (F18, VT2e-B, and WT were seeded in homogeneous conditions and were harvested simultaneously. Tobacco plants were analysed also by optical and electronic microscope in different phases of growth. Germination of transgenic seeds were delayed of three/five days compared to WT in two replicated experiments, suggesting that genetic manipulation may influenced mechanisms leading to germination. In conclusion the genes coding for VT2e-B and the F18 are stably maintained in the seeds and obtained tobacco seeds represent a valid strategy to ferry antigenic proteins to the gut and a promising non-invasive method of vaccination in pig industry.

  14. Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment.

    Science.gov (United States)

    Gao, Caixia; Long, Danfeng; Lenk, Ingo; Nielsen, Klaus Kristian

    2008-10-01

    Agrobacterium-mediated transformation and particle bombardment are the two most widely used methods for genetically modifying grasses. Here, these two systems are compared for transformation efficiency, transgene integration and transgene expression when used to transform tall fescue (Festuca arundinacea Schreb.). The bar gene was used as a selectable marker and selection during tissue culture was performed using 2 mg/l bialaphos in both callus induction and regeneration media. Average transformation efficiency across the four callus lines used in the experiments was 10.5% for Agrobacterium-mediated transformation and 11.5% for particle bombardment. Similar transgene integration patterns and co-integration frequencies of bar and uidA were observed in both gene transfer systems. However, while GUS activity was detected in leaves of 53% of the Agrobacterium transformed lines, only 20% of the bombarded lines showed GUS activity. Thus, Agrobacterium-mediated transformation appears to be the preferred method for producing transgenic tall fescue plants.

  15. Genetic engineering of novel flower colors in floricultural plants: recent advances via transgenic approaches.

    Science.gov (United States)

    Nishihara, Masahiro; Nakatsuka, Takashi

    2010-01-01

    Since the first successful genetic engineering of flower color in petunia, several new techniques have been developed and applied to modify flower color not only in model plants but also in floricultural plants. A typical example is the commercial violet-flowered carnation "Moondust series" developed by Suntry Ltd. and Florigene Ltd. More recently, blue-flowered roses have been successfully produced and are expected to be commercially available in the near future. In recent years, successful modification of flower color by sophisticated regulation of flower-pigment metabolic pathways has become possible. In this chapter, we review recent advances in flower color modification by genetic engineering, especially focusing on the methodology. We have included our own recent results on successful production of flower-color-modified transgenic plants in a model plant, tobacco and an ornamental plant, gentian. Based on these results, genetic engineering of flower color for improvement of floricultural plants is discussed.

  16. Ceramic membranes for gas separation in advanced fossil power plants

    Energy Technology Data Exchange (ETDEWEB)

    Meulenberg, W.A.; Baumann, S.; Ivanova, M.; Gestel, T. van; Bram, M.; Stoever, D. [Forschungszentrum Juelich GmbH (DE). Inst. fuer Energieforschung (IEF)

    2010-07-01

    The reduction or elimination of CO{sub 2} emissions from electricity generation power plants fuelled by coal or gas is a major target in the current socio-economic, environmental and political discussion to reduce green house gas emissions such as CO{sub 2}. This mission can be achieved by introducing gas separation techniques making use of membrane technology, which is, as a rule, associated with significantly lower efficiency losses compared with the conventional separation technologies. Depending on the kind of power plant process different membrane types (ceramic, polymer, metal) can be implemented. The possible technology routes are currently investigated to achieve the emission reduction. They rely on different separation tasks. The CO{sub 2}/N{sub 2} separation is the main target in the post-combustion process. Air separation (O{sub 2}/N{sub 2}) is the focus of the oxyfuel process. In the pre-combustion process an additional H{sub 2}/CO{sub 2} separation is included. Although all separation concepts imply different process requirements they have in common a need in membranes with high permeability, selectivity and stability. In each case CO{sub 2} is obtained in a readily condensable form. CO{sub 2}/N{sub 2} separation membranes like microporous membranes or polymer membranes are applicable in post-combustion stages. In processes with oxyfuel combustion, where the fuel is combusted with pure oxygen, oxygen transport membranes i.e. mixed ionic electronic conducting (MIEC) membranes with mainly perovskite or fluorite structure can be integrated. In the pre-combustion stages of the power plant process, H{sub 2}/CO{sub 2} separation membranes like microporous membranes e.g. doped silica or mixed protonic electronic conductors or metal membranes can be applied. The paper gives an overview about the considered ceramic materials for the different gas separation membranes. The manufacturing of bulk materials as well as supported thin films of these membranes along

  17. Simultaneous Waste Heat and Water Recovery from Power Plant Flue Gases for Advanced Energy Systems

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Dexin [Gas Technology Inst., Des Plaines, IL (United States)

    2016-12-31

    This final report presents the results of a two-year technology development project carried out by a team of participants sponsored by the Department of Energy (DOE). The objective of this project is to develop a membrane-based technology to recover both water and low grade heat from power plant flue gases. Part of the recovered high-purity water and energy can be used directly to replace plant boiler makeup water as well as improving its efficiency, and the remaining part of the recovered water can be used for Flue Gas Desulfurization (FGD), cooling tower water makeup or other plant uses. This advanced version Transport Membrane Condenser (TMC) with lower capital and operating costs can be applied to existing plants economically and can maximize waste heat and water recovery from future Advanced Energy System flue gases with CO2 capture in consideration, which will have higher moisture content that favors the TMC to achieve higher efficiency.

  18. Construction of a Plant Transformation-ready Expression cDNA Library for Thellungiella halophila Using Recombination Cloning

    Institute of Scientific and Technical Information of China (English)

    Wan-Song Ni; Zhi-Yong Lei; Xi Chen; David J. Oliver; Cheng-Bin Xiang

    2007-01-01

    Salt cress (Thellungiella halophila), a close relative of the model plant Arabidopsis thaliana L., is an extremophile that is adapted to harsh saline environments. To mine salt-tolerance genes from this species, we constructed an entry cDNA library from the salt cress plant treated with salt-stress by using a modified cDNA synthesis and an improved recombinationassisted cDNA library construction method that is completely free of manipulations involving restriction enzymes and DNA ligase. This cDNA library construction procedure is significantly simplified and the quality of the cDNA library is improved. This entry cDNA library was subsequently shuttled into the destination binary vector pCB406 designed for plant transformation and expression via recombination-assisted cloning. The library is plant transformation ready and is used to transform Arabidopsis on a large scale in order to create a large collection of transgenic lines for functional gene mining.

  19. Can plant oncogenes inhibit programmed cell death? The rolB oncogene reduces apoptosis-like symptoms in transformed plant cells.

    Science.gov (United States)

    Gorpenchenko, Tatiana Y; Aminin, Dmitry L; Vereshchagina, Yuliya V; Shkryl, Yuri N; Veremeichik, Galina N; Tchernoded, Galina K; Bulgakov, Victor P

    2012-09-01

    The rolB oncogene was previously identified as an important player in ROS metabolism in transformed plant cells. Numerous reports indicate a crucial role for animal oncogenes in apoptotic cell death. Whether plant oncogenes such as rolB can induce programmed cell death (PCD) in transformed plant cells is of particular importance. In this investigation, we used a single-cell assay based on confocal microscopy and fluorescent dyes capable of discriminating between apoptotic and necrotic cells. Our results indicate that the expression of rolB in plant cells was sufficient to decrease the proportion of apoptotic cells in steady-state conditions and diminish the rate of apoptotic cells during induced PCD. These data suggest that plant oncogenes, like animal oncogenes, may be involved in the processes mediating PCD.

  20. Organogenesis and plant selection by kanamycin of strawberry in vitro aiming at genetic transformation

    Directory of Open Access Journals (Sweden)

    Letícia Reis

    2015-08-01

    Full Text Available The success of genetic transformation of the strawberry (Fragaria x ananassa is strongly influenced by the cultivar and depends on efficient regeneration and plant-transformed selection. In plant regeneration, efficient organogenesis depends on the concentrations of plant growth regulators that are present in the culture medium, and the response varies according to the explant type and cultivar. Similarly, in the selection of transformed plants, the sensitivity to selective agents induced by marker genes depends on the concentration of the selective substance in the regeneration medium and must be adjusted according to the chosen cultivar. The aim of this work was to test different combinations and concentrations of TDZ (Thidiazuron and IBA (indolebutyric-acid added to the base medium MS (MURASHIGE; SKOOG, 1962 for shoot regeneration of Camiño Real and Festival cultivars from leaf and petiole explants. In the case of ‘Camiño Real’, 1.0, 1.5 and 2.0 mg L-1 of TDZ combined with 0.1, 0.3 and 0.5 mg L-1 of IBA were tested. In the case of ‘Festival’, 1.0, 1.5 and 2.0 mg L-1 of TDZ combined with 0.1 and 0.3 mg L-1 of IBA were evaluated. Another aim was to define the lowest concentration of the selective agent Kanamycin (marker gene npt II able to inhibit regeneration of the untransformed shoots. For this purpose, Kanamycin doses of 0, 2, 4, 6, 8 and 10 mg L-1 were added to the pre-established regeneration medium. In the petiole explant test, normal shoot regeneration did not occur; thus, only shoot regeneration and the sensitivity to Kanamycin from leaf explants were analyzed. For ‘Camiño Real’ the best shoot regeneration was established with a combination of 2.0 mg L-1 of TDZ + 0.1 mg L-1 of IBA. On the other hand, for ‘Festival’ the best shoot regeneration was established with a combination of 2.5 mg L-1 of TDZ + 0.1 mg L-1 of IBA. As far as Kanamycin sensitivity is concerned, total inhibition of the shoots was observed with 10 mg

  1. Transformations of the Pleiomorphic Plant MTOC during Sporogenesis in the Hepatic Marchantia polymorpha

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Sporogenesis in the hepatic Marchantia polymorpha L. provides an outstanding example of the pleiomorphic nature of the plant microtubule organizing center (MTOC). Microtubules are nucleated from γ-tubulin in MTOCs that change form during mitosis and meiosis. Following entry of cells into the reproductive pathway of sporogenesis, successive rounds of mitosis give rise to packets of 4-16 sporocytes. Mitotic spindles are organized at discrete polar organizers (POs), a type of MTOC that is unique to this group of early divergent land plants. An abrupt and radical transformation in microtubule organization occurs when sporocytes enter meiosis: POs are lost and γ-tubulin is closely associated with surfaces of two large elongated plastids that subsequently divide into four. Migration of the four plastid MTOCs into a tetrahedral arrangement establishes the future spore domains and the division polarity of meiosis. As is typical of many bryophytes,cones of microtubules from the four plastid MTOCs initiate a quadripolar microtubule system (QMS) in meiotic prophase.At this point a transformation in the organization of the MTOCs occurs. The γ-tubulin detaches from plastids and forms a diffuse spheroidal pole in each of the spore domains. The plastids, which are no longer MTOCs, continue to divide. The diffuse MTOCs continue to nucleate cones of microtubules during transformation of the QMS to a bipolar spindle. Following meiosis I, γ-tubulin is associated with nuclear envelopes, and the spindles of meiosis Ⅱ are organized from diffuse MTOCs at the tetrad poles. At simultaneous cytokinesis, radial microtubule systems are organized at nuclear envelope MTOCs in each of the tetrad members.

  2. Potassium chloride and rare earth elements improve plant growth and increase the frequency of the Agrobacterium tumefaciens-mediated plant transformation.

    Science.gov (United States)

    Boyko, Alex; Matsuoka, Aki; Kovalchuk, Igor

    2011-04-01

    Plant transformation efficiency depends on the ability of the transgene to successfully interact with plant host factors. Our previous work and the work of others showed that manipulation of the activity of host factors allows for increased frequency of transformation. Recently we reported that exposure of tobacco plants to increased concentrations of ammonium nitrate increases the frequency of both homologous recombination and plant transgenesis. Here we tested the influence of KCl and salts of rare earth elements, Ce and La on the efficiency of Agrobacterium-mediated plant transformation. We found that exposure to KCl, CeCl(3) and LaCl(3) leads to an increase in recombination frequency in Arabidopsis and tobacco. Plants grown in the presence of CeCl(3) and LaCl(3) had higher biomass, longer roots and greater root number. Analysis of transformation efficiency showed that exposure of tobacco plants to 50 mM KCl resulted in ~6.0-fold increase in the number of regenerated calli and transgenic plants as compared to control plants. Exposure to various concentrations of CeCl(3) showed a maximum increase of ~3.0-fold in both the number of calli and transgenic plants. Segregation analysis showed that exposure to KCl and cerium (III) chloride leads to more frequent integrations of the transgene at a single locus. Analysis of transgene intactness showed better preservation of right T-DNA border during transgene integration. Our data suggest that KCl and CeCl(3) can be effectively used to improve quantity and quality of transgene integrations.

  3. Mapping Soil Carbon from Cradle to Grave: C Transformations of Root Exudates and Plant Litter

    Science.gov (United States)

    Pett-Ridge, J.; Keiluweit, M.; Nuccio, E.; Bougoure, J.; Weber, P. K.; Brodie, E.; Mayali, X.; Shi, S.; Hwang, M.; Thelen, M.; Firestone, M.; Kleber, M.; Nico, P. S.

    2013-12-01

    Carbon cycling in the rhizosphere is a nexus of biophysical interactions between plant roots, microorganisms, and the soil organo-mineral matrix. Plant roots provide 30-40% of soil organic C inputs, accelerate the rate of organic matter mineralization by ~10X, and support an active microhabitat for microbial transformation of soil C. Our research on how roots influence decomposition of soil organic matter in both simplified and complex microcosms uses geochemical characterization, molecular microbiology, isotope tracing, metabolomics and novel imaging approaches (';ChipSIP' and ';STXM-SIMS') to trace the fate of isotopically labelled root exudates and plant tissues. Our previous work suggests root exudates drive O2 limitation, alter metal chemistry and mineralogy, and influence the availability of SOM. Our most recent experiments using synthetic rhizospheres were designed to identify the role of root exudates on ligno-cellulose decomposition in soils. Cultures of 13C/15N-labeled single plant cells (lignin-rich tracheary elements) were added to rhizosphere microcosm soils, and their decomposition followed under the influence of different root exudates using the dual imaging approach ';STXM-SIMS'. Using this combination of X-ray spectromicroscopy and NanoSIMS, we imaged the deconstruction of 13C/15N-labeled ligno-cellulose in situ, and mapped associations of plant cell-derived decomposition products with specific soil minerals. We've also looked at microbial community function in the more complex rhizospheres surrounding roots of the annual grass Avena fatua. Using an isotope array that allows us to follow root C into bacterial, fungal, and microfaunal communities, we tracked the movement of 13C from labeled exudates and 15N from labeled root litter into the soil microbial community. Our results indicate that the microbial communities involved in litter decomposition differ in rhizosphere versus bulk soils, which may have implications for carbon stabilization in soil.

  4. Advancement of safeguards inspection technology for CANDU nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jae Sung; Park, W. S.; Cha, H. R.; Ham, Y. S.; Lee, Y. G.; Kim, K. P.; Hong, Y. D

    1999-04-01

    The objectives of this project are to develop both inspection technology and safeguards instruments, related to CANDU safeguards inspection, through international cooperation, so that those outcomes are to be applied in field inspections of national safeguards. Furthermore, those could contribute to the improvement of verification correctness of IAEA inspections. Considering the level of national inspection technology, it looked not possible to perform national inspections without the joint use of containment and surveillance equipment conjunction with the IAEA. In this connection, basic studies for the successful implementation of national inspections was performed, optimal structure of safeguards inspection was attained, and advancement of safeguards inspection technology was forwarded. The successful implementation of this project contributed to both the improvement of inspection technology on CANDU reactors and the implementation of national inspection to be performed according to the legal framework. In addition, it would be an opportunity to improve the ability of negotiating in equal shares in relation to the IAEA on the occasion of discussing or negotiating the safeguards issues concerned. Now that the national safeguards technology for CANDU reactors was developed, the safeguards criteria, procedure and instruments as to the other item facilities and fabrication facilities should be developed for the perfection of national inspections. It would be desirable that the recommendations proposed and concreted in this study, so as to both cope with the strengthened international safeguards and detect the undeclared nuclear activities, could be applied to national safeguards scheme. (author)

  5. Advancement of safeguards inspection technology for CANDU nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Jae Sung; Park, W. S.; Cha, H. R.; Ham, Y. S.; Lee, Y. G.; Kim, K. P.; Hong, Y. D

    1999-04-01

    The objectives of this project are to develop both inspection technology and safeguards instruments, related to CANDU safeguards inspection, through international cooperation, so that those outcomes are to be applied in field inspections of national safeguards. Furthermore, those could contribute to the improvement of verification correctness of IAEA inspections. Considering the level of national inspection technology, it looked not possible to perform national inspections without the joint use of containment and surveillance equipment conjunction with the IAEA. In this connection, basic studies for the successful implementation of national inspections was performed, optimal structure of safeguards inspection was attained, and advancement of safeguards inspection technology was forwarded. The successful implementation of this project contributed to both the improvement of inspection technology on CANDU reactors and the implementation of national inspection to be performed according to the legal framework. In addition, it would be an opportunity to improve the ability of negotiating in equal shares in relation to the IAEA on the occasion of discussing or negotiating the safeguards issues concerned. Now that the national safeguards technology for CANDU reactors was developed, the safeguards criteria, procedure and instruments as to the other item facilities and fabrication facilities should be developed for the perfection of national inspections. It would be desirable that the recommendations proposed and concreted in this study, so as to both cope with the strengthened international safeguards and detect the undeclared nuclear activities, could be applied to national safeguards scheme. (author)

  6. Projected techno-economic improvements for advanced solar thermal power plants

    Science.gov (United States)

    Fujita, T.; Manvi, R.; Roschke, E. J.

    1979-01-01

    The projected characteristics of solar thermal power plants (with outputs up to 10 MWe) employing promising advanced technology subsystems/components are compared to current (or pre-1985) steam-Rankine systems. Improvements accruing to advanced technology development options are delineated. The improvements derived from advanced systems result primarily from achieving high efficiencies via solar collector systems which (1) capture a large portion of the available insolation and (2) concentrate this captured solar flux to attain high temperatures required for high heat engine/energy conversion performance. The most efficient solar collector systems employ two-axis tracking. Attractive systems include the central receiver/heliostat and the parabolic dish.

  7. Advanced Hydrogen Transport Membranes for Vision 21 Fossil Fuel Plants

    Energy Technology Data Exchange (ETDEWEB)

    Carl R. Evenson; Shane E. Roark

    2006-03-31

    The objective of this project was to develop an environmentally benign, inexpensive, and efficient method for separating hydrogen from gas mixtures produced during industrial processes, such as coal gasification. A family of hydrogen separation membranes was developed including single phase mixed conducting ceramics, ceramic/ceramic composites, cermet membranes, cermet membranes containing a hydrogen permeable metal, and intermediate temperature composite layered membranes. Each membrane type had different operating parameters, advantages, and disadvantages that were documented over the course of the project. Research on these membranes progressed from ceramics to cermets to intermediate temperature composite layered membranes. During this progression performance was increased from 0.01 mL x min{sup -1} x cm{sup -2} up to 423 mL x min{sup -1} x cm{sup -2}. Eltron and team membranes not only developed each membrane type, but also membrane surface catalysis and impurity tolerance, creation of thin film membranes, alternative applications such as membrane promoted alkane dehydrogenation, demonstration of scale-up testing, and complete engineering documentation including process and mechanical considerations necessary for inclusion of Eltron membranes in a full scale integrated gasification combined cycle power plant. The results of this project directly led to a new $15 million program funded by the Department of Energy. This new project will focus exclusively on scale-up of this technology as part of the FutureGen initiative.

  8. Identification of four Sedum plant medicines by Fourier transform infrared spectra

    Directory of Open Access Journals (Sweden)

    Ran Xu

    2012-01-01

    Full Text Available Background: Sedum sarmentosum bunge (SSB., S. lineare Thunb. (SLT, S. erythrostictum migo. (SEM, and S. aizoon L. (SAL were four widely used Chinese traditional drugs or ethnic drugs, which were easy to be confused with each other. Objective: This study aimed at developing a rapid and accurate method to identify the four Sedum plant medicines with very similar appearances and close relationships. Materials and Methods: The herbal medicines employed here were SSB, SLT, SEM, and SAL collected in different places and seasons. Through comparing the infrared (IR spectra of their 70% ethanol extracts, the results showed that the IR spectra of the four plant medicines possessed not only some common characteristics but also certain notable distinctions, such as shapes, numbers, positions, intensity, and ratios of the absorbing peaks. Results: By Fourier transform infrared (FT-IR spectroscopy, the four medicines could be effectively differed, their habitats could be judged preliminarily, and the genetic relationships of the original plants of the four medicines could also be estimated to some extent. Conclusion: The application of FT-IR spectroscopy in crude medicine authentication and quality evaluation deserved to be further emphasized.

  9. Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses

    Institute of Scientific and Technical Information of China (English)

    CHAI A-li; WANG Yi-kai; ZHU Fa-di; SHI Yan-xia; XIE Xue-wen; LI Bao-ju

    2016-01-01

    Identification of plant-pathogenic fungi is time-consuming due to cultivation and microscopic exami-nation and can be influenced by the interpretation of the micro-morphological characters observed.The present investigation aimed to create a simple but sophisticated method for the identification of plant-pathogenic fungi by Fourier transform infrared (FTIR)spectroscopy.In this study,FTIR-attenuated total reflectance (ATR) spectroscopy was used in combination with chemometric analysis for identification of important pathogenic fun-gi of horticultural plants.Mixtures of mycelia and spores from 27 fungal strains belonging to nine different families were collected from liquid PD or solid PDA media cultures and subj ected to FTIR-ATR spectroscopy measurements.The FTIR-ATR spectra ranging from 4 000 to 400 cm-1 were obtained.To classify the FTIR-ATR spectra,cluster analysis was compared with canonical vitiate analysis (CVA)in the spectral regions of 3 050~2 800 and 1 800~900 cm-1 .Results showed that the identification accuracies achieved 97.53% and 9 9.1 8% for the cluster analysis and CVA analysis,respectively,demonstrating the high potential of this tech-nique for fungal strain identification.

  10. Advances in the reintroduction of rare and endangered wild plant species.

    Science.gov (United States)

    Ren, Hai; Jian, ShuGuang; Liu, HongXiao; Zhang, QianMei; Lu, HongFang

    2014-06-01

    Human disturbance and climate change have increased the risk of extinction for rare and endangered wild plant species. One effective way to conserve these rare and endangered species is through reintroduction. In this review, we summarize the advances in wild plant reintroduction from five perspectives: the establishment of reintroduction biology as an important tool for biodiversity conservation; the importance of genetic diversity in reintroduction; reintroduction under global climate change; recruitment limitation in reintroduction; and reintroduction and ecological restoration. In addition, we consider the future of plant reintroduction strategies.

  11. Bench to batch: advances in plant cell culture for producing useful products.

    Science.gov (United States)

    Weathers, Pamela J; Towler, Melissa J; Xu, Jianfeng

    2010-02-01

    Despite significant efforts over nearly 30 years, only a few products produced by in vitro plant cultures have been commercialized. Some new advances in culture methods and metabolic biochemistry have improved the useful potential of plant cell cultures. This review will provide references to recent relevant reviews along with a critical analysis of the latest improvements in plant cell culture, co-cultures, and disposable reactors for production of small secondary product molecules, transgenic proteins, and other products. Some case studies for specific products or production systems are used to illustrate principles.

  12. High-efficiency Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) and regeneration of insect-resistant transgenic plants.

    Science.gov (United States)

    Mehrotra, Meenakshi; Sanyal, Indraneel; Amla, D V

    2011-09-01

    To develop an efficient genetic transformation system of chickpea (Cicer arietinum L.), callus derived from mature embryonic axes of variety P-362 was transformed with Agrobacterium tumefaciens strain LBA4404 harboring p35SGUS-INT plasmid containing the uidA gene encoding β-glucuronidase (GUS) and the nptII gene for kanamycin selection. Various factors affecting transformation efficiency were optimized; as Agrobacterium suspension at OD(600) 0.3 with 48 h of co-cultivation period at 20°C was found optimal for transforming 10-day-old MEA-derived callus. Inclusion of 200 μM acetosyringone, sonication for 4 s with vacuum infiltration for 6 min improved the number of GUS foci per responding explant from 1.0 to 38.6, as determined by histochemical GUS assay. For introducing the insect-resistant trait into chickpea, binary vector pRD400-cry1Ac was also transformed under optimized conditions and 18 T(0) transgenic plants were generated, representing 3.6% transformation frequency. T(0) transgenic plants reflected Mendelian inheritance pattern of transgene segregation in T(1) progeny. PCR, RT-PCR, and Southern hybridization analysis of T(0) and T(1) transgenic plants confirmed stable integration of transgenes into the chickpea genome. The expression level of Bt-Cry protein in T(0) and T(1) transgenic chickpea plants was achieved maximum up to 116 ng mg(-1) of soluble protein, which efficiently causes 100% mortality to second instar larvae of Helicoverpa armigera as analyzed by an insect mortality bioassay. Our results demonstrate an efficient and rapid transformation system of chickpea for producing non-chimeric transgenic plants with high frequency. These findings will certainly accelerate the development of chickpea plants with novel traits.

  13. Transgenic plants from shoot apical meristems of Vitis vinifera L. "Thompson Seedless" via Agrobacterium-mediated transformation.

    Science.gov (United States)

    Dutt, M; Li, Z T; Dhekney, S A; Gray, D J

    2007-12-01

    Shoot apical meristem explants of Vitis vinifera "Thompson Seedless" were used for Agrobacterium-mediated genetic transformation. It was determined that the meristems had to be subjected to a dark growth phase then wounded to obtain transgenic plants. Morphological and histological studies illustrated the role of wounding to expose apical meristem cells for transformation. A bifunctional egfp/nptII fusion gene was used to select kanamycin resistant plants that expressed green fluorescent protein (GFP). Kanamycin at a concentration of 16 mg L(-1) in selection medium resulted in recovery of non-chimeric transgenic plants that uniformly expressed GFP, whereas 8 mg L(-1) kanamycin allowed non-transgenic and/or chimeric plants to develop. Polymerase chain reaction (PCR) and Southern blot analyses confirmed the presence of transgenes and their stable integration into the genome of regenerated plants. Up to 1% of shoot tips produced stable transgenic cultures within 6 weeks of treatment, resulting in a total of 18 independent lines.

  14. USING OF Agrobacterium-MEDIATED TRANSFORMATION FOR THE BIOTECHNOLOGICAL IMPROVEMENT OF COMPOSITAE PLANTS. ІІ. SYNTHESIS OF BIOACTIVE COMPOUNDS IN TRANSGENIC PLANTS AND «HAIRY» ROOTS

    Directory of Open Access Journals (Sweden)

    N. A. Matvieieva

    2015-04-01

    Full Text Available The review focused on the data concerning current state in the field of Compositae “hairy” roots and transgenic plants construction using A.tumefaciens- and A. rhizogenes-mediated transformation to obtain biologically active compounds, including recombinant proteins. The article presents data on the results of genetic transformation of Cichorium intybus, Lactuca sativa, Artemisia annua, Artemisia vulgaris, Calendula officinalis, Withania somnifera and other Compositae plants as well as studies on the artemisinin, flavonoids, polyphenols, fructans and other compounds accumulation in transgenic plants and roots. The data show that the use of biotechnological approaches for construction of "hairy" roots and transgenic plants with new features are of great interest. The possibility of increase in the accumulation of naturally synthesized bioactive compounds and recombinant proteins production via A. tumefaciens and A. rhizogenes-mediated transformation have been shown. In vitro cultivation of transgenic plants characterized by high level of bioactive compounds accumulation and synthesis of recombinant proteins makes it possible to obtain guaranteed pure raw material. Using of biotechnological approaches preserved natural populations of plants is particularly important for rare and endangered plant species.

  15. Advanced conceptual design report: T Plant secondary containment and leak detection upgrades. Project W-259

    Energy Technology Data Exchange (ETDEWEB)

    Hookfin, J.D.

    1995-05-12

    The T Plant facilities in the 200-West Area of the Hanford site were constructed in the early 1940s to produce nuclear materials in support of national defense activities. T Plant includes the 271-T facility, the 221-T facility, and several support facilities (eg, 2706-T), utilities, and tanks/piping systems. T Plant has been recommended as the primary interim decontamination facility for the Hanford site. Project W-259 will provide capital upgrades to the T Plant facilities to comply with Federal and State of Washington environmental regulations for secondary containment and leak detection. This document provides an advanced conceptual design concept that complies with functional requirements for the T Plant Secondary Containment and Leak Detection upgrades.

  16. Functions and toxicity of nickel in plants: recent advances and future prospects

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Cuiyun [Institute of Molecular Ecology, Key Laboratory of Arid and Grassland Ecology, School of Life Sciences, Lanzhou University, Lanzhou (China); Huang, Dejun [School of Life Sciences, Lanzhou University, Lanzhou (China); Liu, Jianquan

    2009-04-15

    Nickel is an essential nutrient for plants. However, the amount of Ni required for normal growth of plants is very low. Hence, with the level of Ni pollution in the environment increasing, it is essential to understand the functional roles and toxic effects of Ni in plants. We briefly review advances in relevant research over the past 20 years. Based on the available data, two new indirect pathways of Ni toxicity in plants are proposed. These are (i) interference with other essential metal ions and (ii) induction of oxidative stress. Research should focus on these mechanisms at the protein and molecular levels. Further research should also be directed at plant species that are capable of accumulating Ni at high concentration, so-called hyperaccumulators. Such species can provide model systems to study the mechanisms of Ni tolerance and can also be used for phytoremediation by removing nickel from polluted environment. (Abstract Copyright [2009], Wiley Periodicals, Inc.)

  17. External costs of silicon carbide fusion power plants compared to other advanced generation technologies

    Energy Technology Data Exchange (ETDEWEB)

    Lechon, Y. E-mail: yolanda.lechon@ciemat.es; Cabal, H.; Saez, R.M.; Hallberg, B.; Aquilonius, K.; Schneider, T.; Lepicard, S.; Ward, D.; Hamacher, T.; Korhonen, R

    2003-09-01

    This study was performed in the framework of the Socio-Economic Research on Fusion (SERF3), which is jointly conducted by Euratom and the fusion associations. Assessments of monetarized external impacts of the fusion fuel-cycle were previously performed (SERF1 and SERF2). Three different power plant designs were studied, with the main difference being the structural materials and cooling system used. In this third phase of the SERF project the external costs of three additional fusion power plant models using silicon carbide as structural material have been analysed. A comparison with other advanced generation technologies expected to be in use around 2050, when the first fusion power plant would be operative, has also been performed. These technologies include advanced fossil technologies, such as Natural Gas Combined Cycle, Pressurised Fluidised Bed Combustion and Integrated Gasification Combined Cycle with carbon sequestration technologies; fuel cells and renewable technologies including geothermal energy, wind energy and photovoltaic systems with energy storage devices. Fusion power plants using silicon carbide as structural material have higher efficiencies than plants using steel and this fact has a very positive effect on the external costs per kW h. These external costs are in the lowest range of the external costs of advanced generation technologies indicating the outstanding environmental performance of fusion power.

  18. Application of generalized Hough transform for detecting sugar beet plant from weed using machine vision method

    Directory of Open Access Journals (Sweden)

    A Bakhshipour Ziaratgahi

    2017-05-01

    Full Text Available Introduction Sugar beet (Beta vulgaris L. as the second most important world’s sugar source after sugarcane is one of the major industrial crops. The presence of weeds in sugar beet fields, especially at early growth stages, results in a substantial decrease in the crop yield. It is very important to efficiently eliminate weeds at early growing stages. The first step of precision weed control is accurate detection of weeds location in the field. This operation can be performed by machine vision techniques. Hough transform is one of the shape feature extraction methods for object tracking in image processing which is basically used to identify lines or other geometrical shapes in an image. Generalized Hough transform (GHT is a modified version of the Hough transform used not only for geometrical forms, but also for detecting any arbitrary shape. This method is based on a pattern matching principle that uses a set of vectors of feature points (usually object edge points to a reference point to construct a pattern. By comparing this pattern with a set pattern, the desired shape is detected. The aim of this study was to identify the sugar beet plant from some common weeds in a field using the GHT. Materials and Methods Images required for this study were taken at the four-leaf stage of sugar beet as the beginning of the critical period of weed control. A shelter was used to avoid direct sunlight and prevent leaf shadows on each other. The obtained images were then introduced to the Image Processing Toolbox of MATLAB programming software for further processing. Green and Red color components were extracted from primary RGB images. In the first step, binary images were obtained by applying the optimal threshold on the G-R images. A comprehensive study of several sugar beet images revealed that there is a unique feature in sugar beet leaves which makes them differentiable from the weeds. The feature observed in all sugar beet plants at the four

  19. An Assessment of Wind Plant Complex Flows Using Advanced Doppler Radar Measurements

    Science.gov (United States)

    Gunter, W. S.; Schroeder, J.; Hirth, B.; Duncan, J.; Guynes, J.

    2015-12-01

    As installed wind energy capacity continues to steadily increase, the need for comprehensive measurements of wind plant complex flows to further reduce the cost of wind energy has been well advertised by the industry as a whole. Such measurements serve diverse perspectives including resource assessment, turbine inflow and power curve validation, wake and wind plant layout model verification, operations and maintenance, and the development of future advanced wind plant control schemes. While various measurement devices have been matured for wind energy applications (e.g. meteorological towers, LIDAR, SODAR), this presentation will focus on the use of advanced Doppler radar systems to observe the complex wind flows within and surrounding wind plants. Advanced Doppler radars can provide the combined advantage of a large analysis footprint (tens of square kilometers) with rapid data analysis updates (a few seconds to one minute) using both single- and dual-Doppler data collection methods. This presentation demonstrates the utility of measurements collected by the Texas Tech University Ka-band (TTUKa) radars to identify complex wind flows occurring within and nearby operational wind plants, and provide reliable forecasts of wind speeds and directions at given locations (i.e. turbine or instrumented tower sites) 45+ seconds in advance. Radar-derived wind maps reveal commonly observed features such as turbine wakes and turbine-to-turbine interaction, high momentum wind speed channels between turbine wakes, turbine array edge effects, transient boundary layer flow structures (such as wind streaks, frontal boundaries, etc.), and the impact of local terrain. Operational turbine or instrumented tower data are merged with the radar analysis to link the observed complex flow features to turbine and wind plant performance.

  20. Interim results of the study of control room crew staffing for advanced passive reactor plants

    Energy Technology Data Exchange (ETDEWEB)

    Hallbert, B.P.; Sebok, A.; Haugset, K. [OECD Halden Reactor Project (Norway)

    1996-03-01

    Differences in the ways in which vendors expect the operations staff to interact with advanced passive plants by vendors have led to a need for reconsideration of the minimum shift staffing requirements of licensed Reactor Operators and Senior Reactor Operators contained in current federal regulations (i.e., 10 CFR 50.54(m)). A research project is being carried out to evaluate the impact(s) of advanced passive plant design and staffing of control room crews on operator and team performance. The purpose of the project is to contribute to the understanding of potential safety issues and provide data to support the development of design review guidance. Two factors are being evaluated across a range of plant operating conditions: control room crew staffing; and characteristics of the operating facility itself, whether it employs conventional or advanced, passive features. This paper presents the results of the first phase of the study conducted at the Loviisa nuclear power station earlier this year. Loviisa served as the conventional plant in this study. Data collection from four crews were collected from a series of design basis scenarios, each crew serving in either a normal or minimum staffing configuration. Results of data analyses show that crews participating in the minimum shift staffing configuration experienced significantly higher workload, had lower situation awareness, demonstrated significantly less effective team performance, and performed more poorly as a crew than the crews participating in the normal shift staffing configuration. The baseline data on crew configurations from the conventional plant setting will be compared with similar data to be collected from the advanced plant setting, and a report prepared providing the results of the entire study.

  1. The lived experience of nurse practitioners practicing within the Transformational Advanced Professional Practice Model: A phenomenological study.

    Science.gov (United States)

    Elliott, Elizabeth C; Walden, Marlene; Young, Anne; Symes, Lene; Fredland, Nina

    2017-06-01

    The purpose of this study was to describe the lived experiences of nurse practitioners (NPs) practicing within the Transformational Advanced Professional Practice (TAPP) Model, a professional practice model (PPM). A descriptive phenomenological analysis using semistructured interviews of 11 NPs across multiple inpatient and outpatient clinical areas at Texas Children's Hospital. Member checking and theming data occurred using Colaizzi's Method concurrently with Mind Mapping technique. Main themes included: (a) transforming professional practice, (b) cultivating the inner self, and (c) mentoring professional transitions. The findings of this study provide qualitative evidence that the TAPP Model influences role transition and professional development. Transforming NP practice within organizations and within the nursing profession itself will take mindfulness with an intentional approach to design PPMs specifically for NPs. ©2017 American Association of Nurse Practitioners.

  2. Plant Growth Experiments in Zeoponic Substrates: Applications for Advanced Life Support Systems

    Science.gov (United States)

    Ming, Douglas W.; Gruener, J. E.; Henderson, K. E.; Steinberg, S. L.; Barta, D. J.; Galindo, C.; Henninger, D. L.

    2001-01-01

    A zeoponic plant-growth system is defined as the cultivation of plants in artificial soils, which have zeolites as a major component (Allen and Ming, 1995). Zeolites are crystalline, hydrated aluminosilicate minerals that have the ability to exchange constituent cations without major change of the mineral structure. Recently, zeoponic systems developed at the National Aeronautics and Space Administration (NASA) slowly release some (Allen et at., 1995) or all of the essential plant-growth nutrients (Ming et at., 1995). These systems have NH4- and K-exchanged clinoptilolite (a natural zeolite) and either natural or synthetic apatite (a calcium phosphate mineral). For the natural apatite system, Ca and P were made available to the plant by the dissolution of apatite. Potassium and NH4-N were made available by ion-exchange reactions involving Ca(2+) from apatite dissolution and K(+) and NH4(+) on zeolitic exchange sites. In addition to NH4-N, K, Ca, and P, the synthetic apatite system also supplied Mg, S, and other micronutrients during dissolution (Figure 1). The overall objective of this research task is to develop zeoponic substrates wherein all plant growth nutrients are supplied by the plant growth medium for several growth seasons with only the addition of water. The substrate is being developed for plant growth in Advanced Life Support (ALS) testbeds (i.e., BioPLEX) and microgravity plant growth experiments. Zeoponic substrates have been used for plant growth experiments on two Space Shuttle flight experiments (STS-60; STS-63; Morrow et aI., 1995). These substrates may be ideally suited for plant growth experiments on the International Space Station and applications in ALS testbeds. However, there are several issues that need to be resolved before zeoponics will be the choice substrate for plant growth experiments in space. The objective of this paper is to provide an overview on recent research directed toward the refinement of zeoponic plant growth substrates.

  3. Karhunen-Loeve Transform and Sparse Representation Based Plant Leaf Disease Recognition

    Directory of Open Access Journals (Sweden)

    Tian Jie

    2013-09-01

    Full Text Available To improve the classification accuracy rate of apple leaf disease images and solve the problem of dimension redundancy in feature extraction, Karhunen-Loeve (K-L transform and sparse representation are applied to apple leaf disease recognition. Firstly 9 color features and 8 texture features of disease leaf images are extracted and taken as feature vectors after dimensionality reduction by the K-L transform. Then, for each of apple mosaic virus, apple rust and apple alternaria leaf spot, 40 apple leaf images are selected as the training samples, whose feature vectors are made up of the dictionary of the sparse representation, respectively. Each testing sample is classified into the class with the minimal residual. The identifying results using the proposed method are analyzed and compared with those of the Support Vector Machine (SVM and original sparse representation method. The average classification accuracy rate of the proposed method is 94.18 %, which confirms its good robustness. In addition, the proposed method not only improves the plant leaf disease classification accuracy but also solves the redundancy problem of the extracted features.

  4. Improvement of environmental aspects of thermal power plant operation by advanced control concepts

    Directory of Open Access Journals (Sweden)

    Mikulandrić Robert

    2012-01-01

    Full Text Available The necessity of the reduction of greenhouse gas emissions, as formulated in the Kyoto Protocol, imposes the need for improving environmental aspects of existing thermal power plants operation. Improvements can be reached either by efficiency increment or by implementation of emission reduction measures. Investments in refurbishment of existing plant components or in plant upgrading by flue gas desulphurization, by primary and secondary measures of nitrogen oxides reduction, or by biomass co-firing, are usually accompanied by modernisation of thermal power plant instrumentation and control system including sensors, equipment diagnostics and advanced controls. Impact of advanced control solutions implementation depends on technical characteristics and status of existing instrumentation and control systems as well as on design characteristics and actual conditions of installed plant components. Evaluation of adequacy of implementation of advanced control concepts is especially important in Western Balkan region where thermal power plants portfolio is rather diversified in terms of size, type and commissioning year and where generally poor maintenance and lack of investments in power generation sector resulted in high greenhouse gases emissions and low efficiency of plants in operation. This paper is intended to present possibilities of implementation of advanced control concepts, and particularly those based on artificial intelligence, in selected thermal power plants in order to increase plant efficiency and to lower pollutants emissions and to comply with environmental quality standards prescribed in large combustion plant directive. [Acknowledgements. This paper has been created within WBalkICT - Supporting Common RTD actions in WBCs for developing Low Cost and Low Risk ICT based solutions for TPPs Energy Efficiency increasing, SEE-ERA.NET plus project in cooperation among partners from IPA SA - Romania, University of Zagreb - Croatia and Vinca

  5. The advanced super critical 700{sup o}C pulverized coal-fired power plant

    Energy Technology Data Exchange (ETDEWEB)

    Kjaer, S.; Klauke, F.; Vanstone, R.; Zeijseink, A.; Weissinger, G.; Kristensen, P.; Meier, J.; Blum, R.; Wieghardt, K. [Tech-Wise A/S, Fredericia (Denmark)

    2001-07-01

    This paper presents the efforts of a large European group of manufacturers, utilities and institutes co-operating in a phased long-term project named 'Advanced 700{sup o}C PF Power Plant'. The first phase started in 1998 based on a grant from the Commission's Thermie programme under the 4th Framework programme. The overall objective of the project is to ensure a role for coal in Europe also in future. The project's targets renewedpublic and political acceptance of coal by improving efficiency and economy of well-proven, super critical pulverised coal-fired technology. Net efficiencies of more than 50% will be reached through development of a super critical steam cycle operating at maximum steam temperatures in the range of 700{sup o}C. Principal efforts are based on development of creep resistant - and expensive - nickel-based materials named super-alloys for the hottest areas of the water/steam cycle. Three benchmarks for theinvestigations have been set up: (i) the net efficiency of the demonstration plant from the present state of the art performance of 44% will be boosted into the range of 50-51% for a plant located inland with a cooling tower and 53-54% for the best seawater-cooled versions. (ii) reductions of investment cost of PF power plant by revising the overall architecture of the plant. (iii) Co-firing of up to 20% biomass with coal. The Advanced 700{sup o}C PF power plant project (or AD700) will improve the competitiveness of coal-fired power generation and give a major reduction of CO{sub 2} from coal-fired power plants in the range of 15% from the best PF power plants presently and up to 40% from older plants. 11 figs., 2 tabs.

  6. Study of Pu consumption in Advanced Light Water Reactors. Evaluation of GE Advanced Boiling Water Reactor plants

    Energy Technology Data Exchange (ETDEWEB)

    1993-05-13

    Timely disposal of the weapons plutonium is of paramount importance to permanently safeguarding this material. GE`s 1300 MWe Advanced Boiling Water Reactor (ABWR) has been designed to utilize fill] core loading of mixed uranium-plutonium oxide fuel. Because of its large core size, a single ABWR reactor is capable of disposing 100 metric tons of plutonium within 15 years of project inception in the spiking mode. The same amount of material could be disposed of in 25 years after the start of the project as spent fuel, again using a single reactor, while operating at 75 percent capacity factor. In either case, the design permits reuse of the stored spent fuel assemblies for electrical energy generation for the remaining life of the plant for another 40 years. Up to 40 percent of the initial plutonium can also be completely destroyed using ABWRS, without reprocessing, either by utilizing six ABWRs over 25 years or by expanding the disposition time to 60 years, the design life of the plants and using two ABWRS. More complete destruction would require the development and testing of a plutonium-base fuel with a non-fertile matrix for an ABWR or use of an Advanced Liquid Metal Reactor (ALMR). The ABWR, in addition, is fully capable of meeting the tritium target production goals with already developed target technology.

  7. Agrobacterium-mediated transformation of the haploid liverwort Marchantia polymorpha L., an emerging model for plant biology.

    Science.gov (United States)

    Ishizaki, Kimitsune; Chiyoda, Shota; Yamato, Katsuyuki T; Kohchi, Takayuki

    2008-07-01

    Agrobacterium-mediated transformation has not been practical in pteridophytes, bryophytes and algae to date, although it is commonly used in model plants including Arabidopsis and rice. Here we present a rapid Agrobacterium-mediated transformation system for the haploid liverwort Marchantia polymorpha L. using immature thalli developed from spores. Hundreds of hygromycin-resistant plants per sporangium were obtained by co-cultivation of immature thalli with Agrobacterium carrying the binary vector that contains a reporter, the beta-glucuronidase (GUS) gene with an intron, and a selection marker, the hygromycin phosphotransferase (hpt) gene. In this system, individual gemmae, which arise asexually from single initial cells, were analyzed as isogenic transformants. GUS activity staining showed that all hygromycin-resistant plants examined expressed the GUS transgene in planta. DNA analyses verified random integration of 1-5 copies of the intact T-DNA between the right and the left borders into the M. polymorpha genome. The efficient and rapid Agrobacterium-mediated transformation of M. polymorpha should provide molecular techniques to facilitate comparative genomics, taking advantage of this unique model plant that retains many features of the common ancestor of land plants.

  8. Advances in Photocatalysis: A Microreview of Visible Light Mediated Ruthenium and Iridium Catalyzed Organic Transformations.

    Science.gov (United States)

    Day, Jon I; Teegardin, Kip; Weaver, Jimmie; Chan, John

    2016-07-15

    Photocatalytic organic transformations utilizing ruthenium and iridium complexes have garnered significant attention due to the access they provide to new synthetic spaces through new reaction mechanisms. A survey of the photophysical data and the diversity of transformations that may be accomplished utilizing commercially available photocatalysts is contained herein.

  9. Acquiring transgenic tobacco plants with insect resistance and glyphosate tolerance by fusion gene transformation.

    Science.gov (United States)

    Sun, He; Lang, Zhihong; Zhu, Li; Huang, Dafang

    2012-10-01

    The advantages of gene 'stacking' or 'pyramiding' are obvious in genetically modified (GM) crops, and several different multi-transgene-stacking methods are available. Using linker peptides for multiple gene transformation is considered to be a good method to meet a variety of needs. In our experiment, the Bt cry1Ah gene, which encodes the insect-resistance protein, and the mG ( 2 ) -epsps gene, which encodes the glyphosate-tolerance protein, were connected by a 2A or LP4/2A linker. Linker 2A is a peptide from the foot-and-mouth disease virus (FMDV) that has self-cleavage activity. LP4 is a peptide from Raphanus sativus seeds that has a recognition site and is cleaved by a protease. LP4/2A is a hybrid peptide that contains the first 9 amino acids of LP4 and 20 amino acids from 2A. We used the linker peptide to construct four coordinated expression vectors: pHAG, pHLAG, pGAH and pGLAH. Two single gene expression vectors, pSAh and pSmG(2), were used as controls. The six expression vectors and the pCAMBIA2301 vector were transferred into tobacco by Agrobacterium tumefaciens-mediated transformation, and 529 transformants were obtained. Molecular detection and bioassay detection data demonstrated that the transgenic tobaccos possessed good pest resistance and glyphosate tolerance. The two genes in the fusion vector were expressed simultaneously. The plants with the genes linked by the LP4/2A peptide showed better pest resistance and glyphosate tolerance than the plants with the genes linked by 2A. The expression level of the two genes linked by LP4/2A was not significantly different from the single gene vector. Key message The expression level of the two genes linked by LP4/2A was higher than those linked by 2A and was not significantly different from the single gene vector.

  10. ANTIOXIDANT PROPERTIES OF METHANOLIC EXTRACTS FROM THE SHOOTS AND ROOTS OF pRi-TRANSFORMED PLANTS OF REHMANNIA GLUTINOSA LIBOSCH.

    Science.gov (United States)

    Piatczak, Ewelina; Dfbska, Marta; Kontek, Bogdan; Olas, Beata; Wysokinskai, Halina

    2016-01-01

    The antioxidant activity of methanolic extracts derived from shoots (HR-shoots) and roots (HR-roots) of pRi-transformed Rehmannia glutinosa plants were determined. The activity was indicated by the ability of the plant extracts to inhibit superoxide anion (O2(-·)) generation and thiobarbituric acid reactive substances (TBARS) production in resting blood platelets and platelets activated by thrombin. The strongest activity was exhibited by the HR-shoot extract (50 μg/mL). The present study also examines the antioxidant properties of the plant extracts against human plasma lipid peroxidation induced by strong biological oxidants: hydrogen peroxide (H2O2) and H2O2/Fe. The study shows that extracts from transformed R. glutinosa plants may be a promising source of natural antioxidants, which would be valuable in various cardiovascular diseases. The extracts may also protect lipids against oxidative modifications.

  11. Alkene Metathesis Catalysis: A Key for Transformations of Unsaturated Plant Oils and Renewable Derivatives

    Directory of Open Access Journals (Sweden)

    Dixneuf Pierre H.

    2016-03-01

    Full Text Available This account presents the importance of ruthenium-catalysed alkene cross-metathesis for the catalytic transformations of biomass derivatives into useful intermediates, especially those developed by the authors in the Rennes (France catalysis team in cooperation with chemical industry. The cross-metathesis of a variety of functional alkenes arising from plant oils, with acrylonitrile and fumaronitrile and followed by catalytic tandem hydrogenation, will be shown to afford linear amino acid derivatives, the precursors of polyamides. The exploration of cross-metathesis of bio-sourced unsaturated nitriles with acrylate with further catalytic hydrogenation has led to offer an excellent route to α,ω-amino acid derivatives. That of fatty aldehydes has led to bifunctional long chain aldehydes and saturated diols. Two ways of access to functional dienes by ruthenium-catalyzed ene-yne cross-metathesis of plant oil alkene derivatives with alkynes and by cross-metathesis of bio-sourced alkenes with allylic chloride followed by catalytic dehydrohalogenation, are reported. Ricinoleate derivatives offer a direct access to chiral dihydropyrans and tetrahydropyrans via ring closing metathesis. Cross-metathesis giving value to terpenes and eugenol for the straightforward synthesis of artificial terpenes and functional eugenol derivatives without C=C bond isomerization are described.

  12. Sulfate removal and sulfur transformation in constructed wetlands: The roles of filling material and plant biomass.

    Science.gov (United States)

    Chen, Yi; Wen, Yue; Zhou, Qi; Huang, Jingang; Vymazal, Jan; Kuschk, Peter

    2016-10-01

    Sulfate in effluent is a challenging issue for wastewater reuse around the world. In this study, sulfur (S) removal and transformation in five batch constructed wetlands (CWs) treating secondary effluent were investigated. The results showed that the presence of the plant cattail (Typha latifolia) had little effect on sulfate removal, while the carbon-rich litter it generated greatly improved sulfate removal, but with limited sulfide accumulation in the pore-water. After sulfate removal, most of the S was deposited with the valence states S (-II) and S (0) on the iron-rich gravel surface, and acid volatile sulfide was the main S sink in the litter-added CWs. High-throughput pyrosequencing revealed that sulfate-reducing bacteria (i.e. Desulfobacter) and sulfide-oxidizing bacteria (i.e. Thiobacillus) were dominant in the litter-added CWs, which led to a sustainable S cycle between sulfate and sulfide. Overall, this study suggests that recycling plant litter and iron-rich filling material in CWs gives an opportunity to utilize the S in the wastewater as both an electron acceptor for sulfate reduction and as an electron donor for nitrate reduction coupled with sulfide oxidation. This leads to the simultaneous removal of sulfate, nitrate, and organics without discharging toxic sulfide into the receiving water body. Copyright © 2016 Elsevier Ltd. All rights reserved.

  13. Impact of high strength electromagnetic fields generated by Tesla transformer on plant cell ultrastructure

    Directory of Open Access Journals (Sweden)

    Anna Rusakova

    2017-09-01

    Full Text Available Non-thermal effects of direct electric fields and alternating electromagnetic fields (EMF have been successfully used in a number of studies and applications in agriculture and biotechnology. Among different kinds of high strength EMF generators, the Tesla transformer (TT is known as a widely applied, low cost, and troubleproof device, which generates EMF in the range of 2–8 MHz. Despite of a number of developed and perspective applications of high strength EMFs in agriculture and biotechnology, the EMFs generated by TT, as well as the 1–50 MHz range of high strength EMF still remain unexplored in the fields of plant physiology, ultrastructure studies and biochemistry. In this work, we have shown that TT-EMFs (4 MHz induced fast stem and petiole bending, disappearance of cell organelles, vacuolar membranes, and increase of a non-photochemical chlorophyll fluorescence quenching in petioles. It is intriguing that such fatal effects can be evoked in plants by EMFs which are well known as harmless for man at the applied strength and frequency.

  14. Agrobacterium rhizogenes-mediated transformation of Superroot-derived Lotus corniculatus plants: a valuable tool for functional genomics

    Directory of Open Access Journals (Sweden)

    Liu Wei

    2009-06-01

    Full Text Available Abstract Background Transgenic approaches provide a powerful tool for gene function investigations in plants. However, some legumes are still recalcitrant to current transformation technologies, limiting the extent to which functional genomic studies can be performed on. Superroot of Lotus corniculatus is a continuous root cloning system allowing direct somatic embryogenesis and mass regeneration of plants. Recently, a technique to obtain transgenic L. corniculatus plants from Superroot-derived leaves through A. tumefaciens-mediated transformation was described. However, transformation efficiency was low and it took about six months from gene transfer to PCR identification. Results In the present study, we developed an A. rhizogenes-mediated transformation of Superroot-derived L. corniculatus for gene function investigation, combining the efficient A. rhizogenes-mediated transformation and the rapid regeneration system of Superroot. The transformation system using A. rhizogenes K599 harbouring pGFPGUSPlus was improved by validating some parameters which may influence the transformation frequency. Using stem sections with one node as explants, a 2-day pre-culture of explants, infection with K599 at OD600 = 0.6, and co-cultivation on medium (pH 5.4 at 22°C for 2 days enhanced the transformation frequency significantly. As proof of concept, Superroot-derived L. corniculatus was transformed with a gene from wheat encoding an Na+/H+ antiporter (TaNHX2 using the described system. Transgenic Superroot plants were obtained and had increased salt tolerance, as expected from the expression of TaNHX2. Conclusion A rapid and efficient tool for gene function investigation in L. corniculatus was developed, combining the simplicity and high efficiency of the Superroot regeneration system and the availability of A. rhizogenes-mediated transformation. This system was improved by validating some parameters influencing the transformation frequency, which could

  15. Transformation of traditional knowledge of medicinal plants: the case of Tyroleans (Austria) who migrated to Australia, Brazil and Peru

    OpenAIRE

    Pirker Heidemarie; Haselmair Ruth; Kuhn Elisabeth; Schunko Christoph; Vogl Christian R

    2012-01-01

    Abstract Background In ethnobotanical research, the investigation into traditional knowledge of medicinal plants in the context of migration has been of increasing interest in recent decades since it is influenced and changed by new environmental and social conditions. It most likely undergoes transformation processes to match the different living circumstances in the new location. This study compares the traditional knowledge of medicinal plants held by Tyroleans – and their descendants – wh...

  16. Advances in molecular marker techniques and their applications in plant sciences.

    Science.gov (United States)

    Agarwal, Milee; Shrivastava, Neeta; Padh, Harish

    2008-04-01

    Detection and analysis of genetic variation can help us to understand the molecular basis of various biological phenomena in plants. Since the entire plant kingdom cannot be covered under sequencing projects, molecular markers and their correlation to phenotypes provide us with requisite landmarks for elucidation of genetic variation. Genetic or DNA based marker techniques such as RFLP (restriction fragment length polymorphism), RAPD (random amplified polymorphic DNA), SSR (simple sequence repeats) and AFLP (amplified fragment length polymorphism) are routinely being used in ecological, evolutionary, taxonomical, phylogenic and genetic studies of plant sciences. These techniques are well established and their advantages as well as limitations have been realized. In recent years, a new class of advanced techniques has emerged, primarily derived from combination of earlier basic techniques. Advanced marker techniques tend to amalgamate advantageous features of several basic techniques. The newer methods also incorporate modifications in the methodology of basic techniques to increase the sensitivity and resolution to detect genetic discontinuity and distinctiveness. The advanced marker techniques also utilize newer class of DNA elements such as retrotransposons, mitochondrial and chloroplast based microsatellites, thereby revealing genetic variation through increased genome coverage. Techniques such as RAPD and AFLP are also being applied to cDNA-based templates to study patterns of gene expression and uncover the genetic basis of biological responses. The review details account of techniques used in identification of markers and their applicability in plant sciences.

  17. Advanced phenotyping and phenotype data analysis for the study of plant growth and development

    Science.gov (United States)

    Rahaman, Md. Matiur; Chen, Dijun; Gillani, Zeeshan; Klukas, Christian; Chen, Ming

    2015-01-01

    Due to an increase in the consumption of food, feed, fuel and to meet global food security needs for the rapidly growing human population, there is a necessity to breed high yielding crops that can adapt to the future climate changes, particularly in developing countries. To solve these global challenges, novel approaches are required to identify quantitative phenotypes and to explain the genetic basis of agriculturally important traits. These advances will facilitate the screening of germplasm with high performance characteristics in resource-limited environments. Recently, plant phenomics has offered and integrated a suite of new technologies, and we are on a path to improve the description of complex plant phenotypes. High-throughput phenotyping platforms have also been developed that capture phenotype data from plants in a non-destructive manner. In this review, we discuss recent developments of high-throughput plant phenotyping infrastructure including imaging techniques and corresponding principles for phenotype data analysis. PMID:26322060

  18. Histological study of organogenesis in Cucumis melo L. after genetic transformation: why is it difficult to obtain transgenic plants?

    Science.gov (United States)

    Chovelon, V; Restier, V; Giovinazzo, N; Dogimont, C; Aarrouf, J

    2011-11-01

    Melon (Cucumis melo L.) is widely considered as a recalcitrant species for genetic transformation. In this study, we developed different regeneration and transformation protocols and we examined the regeneration process at different steps by histological studies. The highest regeneration rate (1.13 ± 0.02 plants per explant) was obtained using cotyledon explants of the 'Védrantais' genotype on Murashige and Skoog (MS) medium supplemented with 0.2 mg/l 6-benzylaminopurine (BAP) and 0.2 mg/l dimethylallylaminopurine (2-iP). Agrobacterium tumefaciens-mediated transformations with the uidA reporter gene were realized on cotyledon explants cultivated in these conditions: 70-90% of explants expressed a transient GUS activity during the early stages of regeneration, however, only few transgenic plants were obtained (1.8-4.5% of stable transformation with the GV2260pBI101 strain). These results revealed a low capacity of melon GUS-positive cells to regenerate transgenic plants. To evaluate the influence of the Agrobacterium infection on plant regeneration, histological analyses were conducted on explants 2, 7, 15, and 28 days after co-culture with the GV2260pBI101 strain. Genetic transformation occurred in epidermal and sub-epidermal cells and reached the meristematic structures expressing a high level of GUS activity during 14 days of culture; but after this period, most of the meristematic structures showed premature cell vacuolization and disorganization. This disruption of the GUS-positive meristematic areas could be responsible of the difficulties encountered to regenerate melon plants after genetic transformation.

  19. An efficient method for Agrobacterium-mediated genetic transformation and plant regeneration in cumin (Cuminum cyminum L.).

    Science.gov (United States)

    Pandey, Sonika; Mishra, Avinash; Patel, Manish Kumar; Jha, Bhavanath

    2013-09-01

    Cumin is an annual herbaceous medicinally important plant having diverse applications. An efficient and reproducible method of Agrobacterium-mediated genetic transformation was herein established for the first time. A direct regeneration method without callus induction was optimised using embryos as explant material in Gamborg's B5 medium supplemented with 0.5-μM 6-benzyladenine and 2.0-μM α-naphthalene acetic acid. About 1,020 embryos (a mean of 255 embryos per batch) were used for the optimisation of transformation conditions. These conditions were an Agrobacterium cell suspension of 0.6 OD600, a co-cultivation time of 72 h, 300-μM acetosyringone and wounding of explants using a razor blade. Pre-cultured elongated embryos were treated using optimised conditions. About 720 embryos (a mean of 180 embryos per batch) were used for transformation and 95 % embryos showed transient β-glucuronidase expression after co-cultivation. Putative transformed embryos were cultured on B5 medium for shoot proliferation and 21 regenerated plants were obtained after selection and allowed to root. T0 plantlets showed β-glucuronidase expression and gene integration was confirmed via PCR amplification of 0.96 and 1.28 kb fragments of the hygromycin-phosphotransferase II and β-glucuronidase genes, respectively. In this study, a transformation efficiency of 1.5 % was demonstrated and a total of 11 transgenic plants were obtained at the hardening stage, however, only four plants acclimatised during hardening. Gene copy number was analysed by Southern blot analysis of hardened plants and single-copy gene integration was observed. This is the first successful attempt of Agrobacterium-mediated genetic transformation of cumin.

  20. Advances in methods for identification and characterization of plant transporter function

    DEFF Research Database (Denmark)

    Larsen, Bo; Xu, Deyang; Halkier, Barbara Ann

    2017-01-01

    Transport proteins are crucial for cellular function at all levels. Numerous importers and exporters facilitate transport of a diverse array of metabolites and ions intra- and intercellularly. Identification of transporter function is essential for understanding biological processes at both...... the cellular and organismal level. Assignment of a functional role to individual transporter proteins or to identify a transporter with a given substrate specificity has notoriously been challenging. Recently, major advances have been achieved in function-driven screens, phenotype-driven screens, and in silico......-based approaches. In this review, we highlight examples that illustrate how new technology and tools have advanced identification and characterization of plant transporter functions....

  1. Operating boundaries of full-scale advanced water reuse treatment plants: many lessons learned from pilot plant experience.

    Science.gov (United States)

    Bele, C; Kumar, Y; Walker, T; Poussade, Y; Zavlanos, V

    2010-01-01

    Three Advanced Water Treatment Plants (AWTP) have recently been built in South East Queensland as part of the Western Corridor Recycled Water Project (WCRWP) producing Purified Recycled Water from secondary treated waste water for the purpose of indirect potable reuse. At Luggage Point, a demonstration plant was primarily operated by the design team for design verification. The investigation program was then extended so that the operating team could investigate possible process optimisation, and operation flexibility. Extending the demonstration plant investigation program enabled monitoring of the long term performance of the microfiltration and reverse osmosis membranes, which did not appear to foul even after more than a year of operation. The investigation primarily identified several ways to optimise the process. It highlighted areas of risk for treated water quality, such as total nitrogen. Ample and rapid swings of salinity from 850 to 3,000 mg/l-TDS were predicted to affect the RO process day-to-day operation and monitoring. Most of the setpoints used for monitoring under HACCP were determined during the pilot plant trials.

  2. Recent advances in the identiifcation and the study of composition and activities of medicinal plants

    Institute of Scientific and Technical Information of China (English)

    Fatiha Brahmi; Sabiha Achat; Naima Guendouze-Bouchefa; Leila Benazzouz-Smail; Mahmoud Fahmi Elsebai; Khodir Madani

    2016-01-01

    ABSTRACT Medicinal and aromatic plants are the foremost source of life economy drugs for the majority of the world’s population. Secondary plant metabolites are essential for life as drugs, fragrances, pigments and food additives. Botanical study of plants is an inevitable step in the ethno-pharmacological approach and it is the reference system since it allows sitting scientifically research that follows from of these new concepts. So, the purpose of many scientists is to advance a new approach useful for identifying plants within a folk pharmacopoeia which deserve the increased research attention of phytochemists and pharmacologists. In the objective to study medicinal plants, usually we start by extraction of the bioactive compounds, which is an essential stage. So, new important extraction processes were elaborated to recover bioactive constituants from herbal plants by augmenting the yield at inferior fee. Recent approachs and methods used in isolation and characterization of active compounds from herbal preparations are also given according to their important roles. Besides, in the present work, an update on some biological activities properties of medicinal plants is reviewed.

  3. Recent advances in the identification and the study of composition and activities of medicinal plants

    Directory of Open Access Journals (Sweden)

    Fatiha Brahmi

    2016-11-01

    Full Text Available Medicinal and aromatic plants are the foremost source of life economy drugs for the majority of the world’s population. Secondary plant metabolites are essential for life as drugs, fragrances, pigments and food additives. Botanical study of plants is an inevitable step in the ethnopharmacological approach and it is the reference system since it allows sitting scientifically research that follows from of these new concepts. So, the purpose of many scientists is to advance a new approach useful for identifying plants within a folk pharmacopoeia which deserve the increased research attention of phytochemists and pharmacologists. In the objective to study medicinal plants, usually we start by extraction of the bioactive compounds, which is an essential stage. So, new important extraction processes were elaborated to recover bioactive constituants from herbal plants by augmenting the yield at inferior fee. Recent approachs and methods used in isolation and characterization of active compounds from herbal preparations are also given according to their important roles. Besides, in the present work, an update on some biological activities properties of medicinal plants is reviewed.

  4. On the advancements of conformal transformations and their associated symmetries in geometry and theoretical physics

    Energy Technology Data Exchange (ETDEWEB)

    Kastrup, H.A. [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Theory Group

    2008-08-15

    The historical developments of conformal transformations and symmetries are sketched: Their origin from stereographic projections of the globe, their blossoming in two dimensions within the eld of analytic complex functions, the generic role of transformations by reciprocal radii in dimensions higher than two and their linearization in terms of polyspherical coordinates by Darboux, Weyl's attempt to extend General Relativity, the slow rise of nite dimensional conformal transformations in classical eld theories and the problem of their interpretation, then since about 1970 the rapid spread of their acceptance for asymptotic and structural problems in quantum eld theories and beyond, up to the current AdS=CFT conjecture. The occasion for the present article: hundred years ago Bateman and Cunningham discovered the form invariance of Maxwell's equations for electromagnetism with respect to conformal space-time transformations. (orig.)

  5. Directly Transforming PCR-Amplified DNA Fragments into Plant Cells Is a Versatile System That Facilitates the Transient Expression Assay

    Science.gov (United States)

    Lu, Yuming; Chen, Xi; Wu, Yuxuan; Wang, Yanping; He, Yuqing; Wu, Yan

    2013-01-01

    A circular plasmid containing a gene coding sequence has been broadly used for studying gene regulation in cells. However, to accommodate a quick screen plasmid construction and preparation can be time consuming. Here we report a PCR amplified dsDNA fragments (PCR-fragments) based transient expression system (PCR-TES) for suiting in the study of gene regulation in plant cells. Instead of transforming plasmids into plant cells, transient expression of PCR-fragments can be applicable. The transformation efficiency and expression property of PCR-fragments are comparable to transformation using plasmids. We analyzed the transformation efficiency in PCR-TES at transcription and protein levels. Our results indicate that the PCR-TES is as versatile as the conventional transformation system using plasmid DNA. Through reconstituting PYR1-mediated ABA signaling pathway in Arabidopsis mesophyll protoplasts, we were not only validating the practicality of PCR-TES but also screening potential candidates of CDPK family members which might be involved in the ABA signaling. Moreover, we determined that phosphorylation of ABF2 by CPK4 could be mediated by ABA-induced PYR1 and ABI1, demonstrating a crucial role of CDPKs in the ABA signaling. In summary, PCR-TES can be applicable to facilitate analyzing gene regulation and for the screen of putative regulatory molecules at the high throughput level in plant cells. PMID:23468926

  6. Directly transforming PCR-amplified DNA fragments into plant cells is a versatile system that facilitates the transient expression assay.

    Directory of Open Access Journals (Sweden)

    Yuming Lu

    Full Text Available A circular plasmid containing a gene coding sequence has been broadly used for studying gene regulation in cells. However, to accommodate a quick screen plasmid construction and preparation can be time consuming. Here we report a PCR amplified dsDNA fragments (PCR-fragments based transient expression system (PCR-TES for suiting in the study of gene regulation in plant cells. Instead of transforming plasmids into plant cells, transient expression of PCR-fragments can be applicable. The transformation efficiency and expression property of PCR-fragments are comparable to transformation using plasmids. We analyzed the transformation efficiency in PCR-TES at transcription and protein levels. Our results indicate that the PCR-TES is as versatile as the conventional transformation system using plasmid DNA. Through reconstituting PYR1-mediated ABA signaling pathway in Arabidopsis mesophyll protoplasts, we were not only validating the practicality of PCR-TES but also screening potential candidates of CDPK family members which might be involved in the ABA signaling. Moreover, we determined that phosphorylation of ABF2 by CPK4 could be mediated by ABA-induced PYR1 and ABI1, demonstrating a crucial role of CDPKs in the ABA signaling. In summary, PCR-TES can be applicable to facilitate analyzing gene regulation and for the screen of putative regulatory molecules at the high throughput level in plant cells.

  7. Water mediated alterations in gravity signal transform phytofilertation capability in hydroponic plants

    Science.gov (United States)

    Singh, Yogranjan; Singh Marabi, Rakesh; Satpute, Gyanesh Kumar; Mishra, Stuti

    2012-07-01

    An exorbitant sum of different synthetic molecules of chemicals including dyes and pigments are discharged into the environment, mainly via industrial effluents every year worldwide. The physical-chemical treatments for remediation viz adsorption, precipitation, ion exchange or filtration have proved to be disadvantageous because of high cost, low efficiency and inapplicability to a wide variety of dyes, or the formation of by-products and thereby creating waste disposal problems. Similarly the limited ability of micro-organisms to degrade xenobiotic especially sulphonoaromatic compounds, limits the efficiency and, therefore, the use of conventional wastewater treatment plants. In this context, the development of alternative biological treatments to eliminate these pollutants from industrial effluents is an important requirement. Plant metabolism, is extremely diverse and can be exploited to treat recalcitrant pollutants, not degradable by bacteria or fungi and can act as an important global sink for environmental pollutants. The presence of putative metabolites, in leaves of hydrophytes has been observed, indicating the transformation of several xenobiotics. A diverse range of the enzymes involved in the early stages of the detoxification process are closely associated with the redox biochemistry of the cell. The activities of enzymes such as glutathione transferases, peroxidases and cytochrome P450 monooxygenases and its multigenic family have implications with respect to the maintenance of redox homeostasis. Besides activating xenobiotics, cytochromes P450 is involved vitally in cell signaling for counteracting buoyant balance. Signal transduction cascades, including the role of cytochrome P450 monooxygenases in responding to gravitational cues, appear to be affected by buoyancy as well. Gravitropism is the orientation of growth in response to gravity and involves the perception of the gravitational force in the columella cells of the root cap where the primary

  8. Plant-based food and feed protein structure changes induced by gene-transformation, heating and bio-ethanol processing: a synchrotron-based molecular structure and nutrition research program.

    Science.gov (United States)

    Yu, Peiqiang

    2010-11-01

    Unlike traditional "wet" analytical methods which during processing for analysis often result in destruction or alteration of the intrinsic protein structures, advanced synchrotron radiation-based Fourier transform infrared microspectroscopy has been developed as a rapid and nondestructive and bioanalytical technique. This cutting-edge synchrotron-based bioanalytical technology, taking advantages of synchrotron light brightness (million times brighter than sun), is capable of exploring the molecular chemistry or structure of a biological tissue without destruction inherent structures at ultra-spatial resolutions. In this article, a novel approach is introduced to show the potential of the advanced synchrotron-based analytical technology, which can be used to study plant-based food or feed protein molecular structure in relation to nutrient utilization and availability. Recent progress was reported on using synchrotron-based bioanalytical technique synchrotron radiation-based Fourier transform infrared microspectroscopy and diffused reflectance infrared Fourier transform spectroscopy to detect the effects of gene-transformation (Application 1), autoclaving (Application 2), and bio-ethanol processing (Application 3) on plant-based food and feed protein structure changes on a molecular basis. The synchrotron-based technology provides a new approach for plant-based protein structure research at ultra-spatial resolutions at cellular and molecular levels.

  9. Impact of microbial/plant interactions on the transformation of polycyclic aromatic hydrocarbons in rhizosphere of Festuca arundinacea.

    Science.gov (United States)

    Ho, C H; Applegate, B; Banks, M K

    2007-01-01

    The promotion of polycyclic aromatic hydrocarbon (PAH) degradation was demonstrated in the rhizosphere of Festuca arundinacea with Pseudomonas fluorescens. P. fluorescens 5RL more significantly interacted with salicylate and dextrose in the agar containing tall fescue than agar without plant roots. Although the presence of tall fescue did not promote catabolic enzyme induction in the absence of salicylate, an increase in dioxygenase activity relative to no plant controls implies that this plant may enhance the degradation of PAHs or facilitate the genotypes that are capable of transforming PAH in the rhizosphere.

  10. The advanced supercritical 700 C pulverised coal-fired power plant

    Energy Technology Data Exchange (ETDEWEB)

    Kjaer, S.; Kristensen, P. [Tech-wise A/S, Fredericia (Denmark); Klauke, F. [Babcock Borsig Power Energy, Oberhausen (Germany); Vanstone, R. [ALSTOM Power UK Ltd., Rugby (United Kingdom); Zeijseink, A. [KEMA Nederland B.V., Arnhem (Netherlands); Weissinger, G. [ALSTOM Power Boilers GmbH, Stuttgart (Germany); Meier, J. [ALSTOM Power Ltd., Baden (Switzerland); Blum, R. [Elsam A/S, Fredericia (Denmark); Wieghardt, K. [Siemens, Muelheim (Germany)

    2002-07-01

    This paper presents the joint efforts of a large European group of manufacturers, utilities and institutes co-operating in a phased long-term project named 'Advanced 700 C PF Power Plant'. Net efficiencies of more than 50% will be reached through development of a super critical steam cycle operating at maximum steam temperatures in the range of 700 C. The principal efforts are based on development of creep resistant - and expensive - Nickel-based materials. (orig.) [German] Der Beitrag beschreibt die gemeinsamen Anstrengungen einer grossen Gruppe europaeischer Kraftwerksbauer, Kraftwerksbetreiber und Institute, die in einem gestuften langfristigen Projekt mit dem Titel 'Advanced 700 C PF Power Plant' zusammenarbeiten. Nettowirkungsgrade von mehr als 50% sollen durch die Entwicklung eines ueberkritischen Dampfkreislaufs erreicht werden, der mit maximalen Dampftemperaturen in der Groessenordnung von 700 C arbeitet. Die Hauptbemuehungen gelten der Entwicklung von kriechfesten und aufwaendigen Werkstoffen auf Nickelbasis, die als Superlegierungen bezeichnet werden. (orig.)

  11. Advanced Reactor Licensing: Experience with Digital I&C Technology in Evolutionary Plants

    Energy Technology Data Exchange (ETDEWEB)

    Wood, RT

    2004-09-27

    This report presents the findings from a study of experience with digital instrumentation and controls (I&C) technology in evolutionary nuclear power plants. In particular, this study evaluated regulatory approaches employed by the international nuclear power community for licensing advanced l&C systems and identified lessons learned. The report (1) gives an overview of the modern l&C technologies employed at numerous evolutionary nuclear power plants, (2) identifies performance experience derived from those applications, (3) discusses regulatory processes employed and issues that have arisen, (4) captures lessons learned from performance and regulatory experience, (5) suggests anticipated issues that may arise from international near-term deployment of reactor concepts, and (6) offers conclusions and recommendations for potential activities to support advanced reactor licensing in the United States.

  12. Manipulating photorespiration to increase plant productivity: recent advances and perspectives for crop improvement.

    Science.gov (United States)

    Betti, Marco; Bauwe, Hermann; Busch, Florian A; Fernie, Alisdair R; Keech, Olivier; Levey, Myles; Ort, Donald R; Parry, Martin A J; Sage, Rowan; Timm, Stefan; Walker, Berkley; Weber, Andreas P M

    2016-05-01

    Recycling of the 2-phosphoglycolate generated by the oxygenase reaction of Rubisco requires a complex and energy-consuming set of reactions collectively known as the photorespiratory cycle. Several approaches aimed at reducing the rates of photorespiratory energy or carbon loss have been proposed, based either on screening for natural variation or by means of genetic engineering. Recent work indicates that plant yield can be substantially improved by the alteration of photorespiratory fluxes or by engineering artificial bypasses to photorespiration. However, there is also evidence indicating that, under certain environmental and/or nutritional conditions, reduced photorespiratory capacity may be detrimental to plant performance. Here we summarize recent advances obtained in photorespiratory engineering and discuss prospects for these advances to be transferred to major crops to help address the globally increasing demand for food and biomass production.

  13. Natural transformation in plant breeding - a biotechnological platform for quality improvement of ornamental, agricultural and medicinal plants

    DEFF Research Database (Denmark)

    Lütken, Henrik Vlk; Hegelund, Josefine Nymark; Himmelboe, Martin;

    2015-01-01

    Compactness is a desirable trait in ornamental plant breeding because it is preferred by producers, distributors and consumers. Presently, in ornamental plant production growth of many potted plants is regulated by application of chemical growth retardants, several of which are harmful to both......, decreased plant height, short internodes, reduced apical dominance and changes in flower characteristics. Several of these traits improve ornamental plant quality and may also benefit characteristics useful in agricultural field crops. In addition, a number of regenerated plants derived from hairy roots...

  14. Water quality transformations during soil aquifer treatment at the Mesa Northwest Water Reclamation Plant, USA.

    Science.gov (United States)

    Fox, P; Narayanaswamy, K; Genz, A; Drewes, J E

    2001-01-01

    Water quality transformations during soil aquifer treatment at the Mesa Northwest Water Reclamation Plant (NWWRP) were evaluated by sampling a network of groundwater monitoring wells located within the reclaimed water plume. The Mesa Northwest Water Reclamation Plant has used soil aquifer treatment (SAT) since it began operation in 1990 and the recovery of reclaimed water from the impacted groundwater has been minimal. Groundwater samples obtained represent travel times from several days to greater than five years. Samples were analyzed for a wide range of organic and inorganic constituents. Sulfate was used as a tracer to estimate travel times and define reclaimed water plume movement. Dissolved organic carbon concentrations were reduced to approximately 1 mg/L after 12 to 24 months of soil aquifer treatment with an applied DOC concentration from the NWWRP of 5 to 7 mg/L. The specific ultraviolet absorbance (SUVA) increased during initial soil aquifer treatment on a time-scale of days and then decreased as longer term soil aquifer treatment removed UV absorbing compounds. The trihalomethane formation potential (THMFP) was a function of the dissolved organic carbon concentration and ranged from 50 to 65 micrograms THMFP/mg DOC. Analysis of trace organics revealed that the majority of trace organics were removed as DOC was removed with the exception of organic iodine. The majority of nitrogen was applied as nitrate-nitrogen and the reclaimed water plume had lower nitrate-nitrogen concentrations as compared to the background groundwater. The average dissolved organic carbon concentrations in the reclaimed water plume were less than 50% of the drinking water dissolved organic concentrations from which the reclaimed water originated.

  15. Comparison of advanced engines for parabolic dish solar thermal power plants

    Science.gov (United States)

    Fujita, T.; Bowyer, J. M.; Gajanana, B. C.

    1980-01-01

    A paraboloidal dish solar thermal power plant produces electrical energy by a two-step conversion process. The collector subsystem is composed of a two-axis tracking paraboloidal concentrator and a cavity receiver. The concentrator focuses intercepted sunlight (direct, normal insolation) into a cavity receiver whose aperture encircles the focal point of the concentrator. At the internal wall of the receiver the electromagnetic radiation is converted to thermal energy. A heat engine/generator assembly then converts the thermal energy captured by the receiver to electricity. Developmental activity has been concentrated on small power modules which employ 11- to 12-meter diameter dishes to generate nominal power levels of approximately 20 kWe. A comparison of advanced heat engines for the dish power module is presented in terms of the performance potential of each engine with its requirements for advanced technology development. Three advanced engine possibilities are the Brayton (gas turbine), Brayton/Rankine combined cycle, and Stirling engines.

  16. Recent advances towards development and commercialization of plant cell culture processes for the synthesis of biomolecules.

    Science.gov (United States)

    Wilson, Sarah A; Roberts, Susan C

    2012-04-01

    Plant cell culture systems were initially explored for use in commercial synthesis of several high-value secondary metabolites, allowing for sustainable production that was not limited by the low yields associated with natural harvest or the high cost associated with complex chemical synthesis. Although there have been some commercial successes, most notably paclitaxel production from Taxus sp., process limitations exist with regards to low product yields and inherent production variability. A variety of strategies are being developed to overcome these limitations including elicitation, in situ product removal and metabolic engineering with single genes and transcription factors. Recently, the plant cell culture production platform has been extended to pharmaceutically active heterologous proteins. Plant systems are beneficial because they are able to produce complex proteins that are properly glycosylated, folded and assembled without the risk of contamination by toxins that are associated with mammalian or microbial production systems. Additionally, plant cell culture isolates transgenic material from the environment, allows for more controllable conditions over field-grown crops and promotes secretion of proteins to the medium, reducing downstream purification costs. Despite these benefits, the increase in cost of heterologous protein synthesis in plant cell culture as opposed to field-grown crops is significant and therefore processes must be optimized with regard to maximizing secretion and enhancing protein stability in the cell culture media. This review discusses recent advancements in plant cell culture processing technology, focusing on progress towards overcoming the problems associated with commercialization of these production systems and highlighting recent commercial successes.

  17. [Research advances in iron and zinc transfer from soil to plant in intercropping systems].

    Science.gov (United States)

    Xia, Hai-yong; Xue, Yan-fang; Meng, Wei-wei; Yu, Li-min; Liu, Ling-yan; Zhang, Zheng

    2015-04-01

    Intercropping facilitates the efficient utilization of land, light, water and nutrients. It is, therefore, important to increase the biodiversity of farmland and to develop sustainable ecological agriculture in both theory and practice. Intercropping helps improve the mobilization and uptake of soil iron (Fe) and zinc (Zn) and corresponding nutritional status in the plants, thus achieving grain micronutrient biofortification. In this review, phenomena of the improvement of Fe and Zn nutrition in dicotyledonous plants as affected by intercropping with gramineous plants (e.g. maize/peanut intercropping) were summarized. Moreover, the possible mechanisms in relation to interspecific rhizosphere molecular and physiological processes, as well as the changes in interspecific root morphology and distribution and microorganisms in the rhizosphere were elucidated. The accumulation, transfer and distribution of Fe and Zn in the plants in intercropping systems were also reviewed. The possible affecting factors on nutrients of Fe and Zn were analyzed. Based on the present advances in the mobilization and acquisition of soil Fe and Zn, and their accumulation and distribution in plants as well as the related management and environment influence factors, some new research questions were pointed out. Quantitative analysis, dynamic and systemic researches and field studies on Fe and Zn transfer from soil to plant in intercropping systems should be strengthened in the future.

  18. Theoretical comparison of single-stage and advanced absorption heat transformers used to increase solar pond's temperature

    Energy Technology Data Exchange (ETDEWEB)

    Rivera, W; Best, Roberto [Centro de Investigacion en Energia-UNAM, Temixco, Morelos (Mexico)

    2000-07-01

    Mathematical models of single-stage and advanced absorption heat transformers operating with the water/Carrol{sup T}M mixture were developed to simulate the performance of these systems coupled to a solar pond in order to increase the temperature of the useful heat produced by solar ponds. The results showed that the single-stage and the double absorption heat transformer are the most promising configuration to be coupled to solar ponds. With single-stage heat transformers it is possible to increase solar pond's temperature until 50 Celsius degrees with coefficients of performance of about 0.48 and with double absorption heat transformers until 100 Celsius degrees with coefficients of performance of 0.33. [Spanish] Se desarrollaron modelos matematicos de una sola etapa y transformadores avanzados de absorcion de calor operando con la mezcla agua/Carrol{sup T}M para simular el rendimiento de estos sistemas acoplados a un estanque solar con el objeto de aumentar la temperatura del calor util producido por los estanques solares. Los resultados mostraron que la etapa sencilla y el transformador de calor de absorcion doble son la configuracion mas prometedora para ser acoplado a estanques solares. Con los transformadores de calor de una sola etapa es posible aumentar la temperatura del estanque solar hasta 50 grados Celsius con coeficientes de rendimiento de alrededor de 0.48 y con transformadores de calor de doble absorcion hasta 100 grados Celsius con coeficientes de rendimiento de 0.33.

  19. Assessment of fiber optic sensors and other advanced sensing technologies for nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Hashemian, H.M. [Analysis and Measurement Services Corporation, Knoxville, TN (United States)

    1996-03-01

    As a result of problems such as calibration drift in nuclear plant pressure sensors and the recent oil loss syndrome in some models of Rosemount pressure transmitters, the nuclear industry has become interested in fiber optic pressure sensors. Fiber optic sensing technologies have been considered for the development of advanced instrumentation and control (I&C) systems for the next generation of reactors and in older plants which are retrofitted with new I&C systems. This paper presents the results of a six-month Phase I study to establish the state-of-the-art in fiber optic pressure sensing. This study involved a literature review, contact with experts in the field, an industrial survey, a site visit to a fiber optic sensor manufacturer, and laboratory testing of a fiber optic pressure sensor. The laboratory work involved both static and dynamic performance tests. This initial Phase I study has recently been granted a two-year extension by the U.S. Nuclear Regulatory Commission (NRC). The next phase will evaluate fiber optic pressure sensors in specific nuclear plant applications in addition to other advanced methods for monitoring critical nuclear plant equipment.

  20. Advanced Optical Signal Processing using Time Lens based Optical Fourier Transformation

    DEFF Research Database (Denmark)

    Guan, Pengyu; Røge, Kasper Meldgaard; Lillieholm, Mads

    2016-01-01

    An overview of recent progress on time lens based advanced optical signal processing is presented, with a special focus on all-optical ultrafast 640 Gbit/s all-channel serial-to-parallel conversion, and scalable WDM regeneration....

  1. 植物内生菌的研究进展%Research Advance on Plant Endophyte

    Institute of Scientific and Technical Information of China (English)

    王莉衡

    2011-01-01

    植物内生是菌是一种新型的微生物资源,具有重大的研究意义和潜在的应用价值,近年来成为微生物资源研究的热点之一.对植物内生菌的研究进展和生物学作用进行了综述,以期更好地了解和研究植物内生菌.%Plant endophytes are the new microorganism resources with great investigative significance and potential applied value, which have become one of the most popular research topics in recent years. In this paper, general situation and research advances and biological functions of plant endophyte were summarized for further understanding of interactions of endophytes and host plants.

  2. Ca2+ transport in plant cells and mechanisms of transformation of phytochrome-induced photosignals

    Science.gov (United States)

    Volotovski, Igor D.

    1995-01-01

    The recent data on the influence of phytochrome on the efficiency of Ca2+ translocation across the membranes of oat protoplasts are given. Ca2+ uptake in the protoplasts was shown to be influenced by the red light (R) illumination. This effect was reverted by the following far-red light (FR) illumination. To elucidate the sensitivity to phytochrome-controlling action the screening between the mechanisms of Ca2+ transport across the plasma membranes of oat protoplasts, Na+/Ca2+ and Ca2+/H+ exchangers, Ca2+-pump and Ca2+-channel was done. It was established that phytochrome modulated the activity of Na+/Ca2+-exchanger and Ca2+-pump. The light-mediated oscillations of cytoplasmic Ca2+ concentration in the oat protoplasts were demonstrated using fluorescence probe quin2 loaded into the cells and laser monitoring of fluorescence signal. The evidences were obtained that the oscillations were not the result of the elevation of cytoplasmic Ca2+ concentration and had no connection with Ca2+ pool of mitochondria. The possibility of the relation between the Ca2+ oscillations and phosphoinositide metabolism in plant cell membranes is analyzed. The mechanisms of transformation of primary phytochrome signal into biological effects were discussed.

  3. Transformation of mercury speciation through the SCR system in power plants

    Institute of Scientific and Technical Information of China (English)

    YANG Hong-min; PAN Wei-ping

    2007-01-01

    Coal-fired utility boilers are now identified as the largest source of mercury in the United States. There is speculation that the installation of selective catalytic reduction (SCR) system for reduction of NOx can also prompt the oxidation and removal of mercury.In this paper, tests at six full-scale power plants with similar type of the SCR systems are conducted to investigate the effect of the SCR on the transformation of mercury speciation. The results show that the SCR system can achieve more than 70%-80% oxidation of elemental mercury and enhance the mercury removal ability in these units. The oxidation of elemental mercury in the SCR system strongly depends on the coal properties and the operation conditions of the SCR systems. The content of chloride in the coal is the key factor for the oxidization process and the maximum oxidation of elemental mercury is found when chloride content changes from 400 to 600 ppm. The sulfur content is no significant impact on oxidation of elemental mercury.

  4. Transformation of mercury speciation through the SCR system in power plants.

    Science.gov (United States)

    Yang, Hong-min; Pan, Wei-ping

    2007-01-01

    Coal-fired utility boilers are now identified as the largest source of mercury in the United States. There is speculation that the installation of selective catalytic reduction (SCR) system for reduction of NOx can also prompt the oxidation and removal of mercury. In this paper, tests at six full-scale power plants with similar type of the SCR systems are conducted to investigate the effect of the SCR on the transformation of mercury speciation. The results show that the SCR system can achieve more than 70%-80% oxidation of elemental mercury and enhance the mercury removal ability in these units. The oxidation of elemental mercury in the SCR system strongly depends on the coal properties and the operation conditions of the SCR systems. The content of chloride in the coal is the key factor for the oxidization process and the maximum oxidation of elemental mercury is found when chloride content changes from 400 to 600 ppm. The sulfur content is no significant impact on oxidation of elemental mercury.

  5. Advanced Grid-Friendly Controls Demonstration Project for Utility-Scale PV Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Gevorgian, Vahan [National Renewable Energy Lab. (NREL), Golden, CO (United States); O' Neill, Barbara [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2016-01-21

    A typical photovoltaic (PV) power plant consists of multiple power electronic inverters and can contribute to grid stability and reliability through sophisticated 'grid-friendly' controls. The availability and dissemination of actual test data showing the viability of advanced utility-scale PV controls among all industry stakeholders can leverage PV's value from being simply an energy resource to providing additional ancillary services that range from variability smoothing and frequency regulation to power quality. Strategically partnering with a selected utility and/or PV power plant operator is a key condition for a successful demonstration project. The U.S. Department of Energy's (DOE's) Solar Energy Technologies Office selected the National Renewable Energy Laboratory (NREL) to be a principal investigator in a two-year project with goals to (1) identify a potential partner(s), (2) develop a detailed scope of work and test plan for a field project to demonstrate the gird-friendly capabilities of utility-scale PV power plants, (3) facilitate conducting actual demonstration tests, and (4) disseminate test results among industry stakeholders via a joint NREL/DOE publication and participation in relevant technical conferences. The project implementation took place in FY 2014 and FY 2015. In FY14, NREL established collaborations with AES and First Solar Electric, LLC, to conduct demonstration testing on their utility-scale PV power plants in Puerto Rico and Texas, respectively, and developed test plans for each partner. Both Puerto Rico Electric Power Authority and the Electric Reliability Council of Texas expressed interest in this project because of the importance of such advanced controls for the reliable operation of their power systems under high penetration levels of variable renewable generation. During FY15, testing was completed on both plants, and a large amount of test data was produced and analyzed that demonstrates the ability of

  6. The Attending Nurse Caring Model: integrating theory, evidence and advanced caring-healing therapeutics for transforming professional practice.

    Science.gov (United States)

    Watson, Jean; Foster, Roxie

    2003-05-01

    This paper presents a proposed model: The Attending Nursing Caring Model (ANCM) as an exemplar for advancing and transforming nursing practice within a reflective, theoretical and evidence-based context. Watson's theory of human caring is used as a guide for integrating theory, evidence and advanced therapeutics in the area of children's pain. The ANCM is offered as a programme for renewing the profession and its professional practices of caring-healing arts and science, during an era of decline, shortages, and crises in care, safety, and hospital and health reform. The ANCM elevates contemporary nursing's caring values, relationships, therapeutics and responsibilities to a higher/deeper order of caring science and professionalism, intersecting with other professions, while sustaining the finest of its heritage and traditions of healing.

  7. Application of Transformational Leadership Principles in the Development and Integration of Palliative Care Within an Advanced Heart Failure Program.

    Science.gov (United States)

    George, Susan; Leasure, A Renee

    2016-01-01

    Heart failure (HF) is a major health problem in United States, and it has reached epidemic proportions. Heart failure is associated with significant morbidity, mortality, and cost. Although the prognosis of HF is worse than many forms of cancer, many patients, families, and clinicians are unaware of the dire prognosis. As the disease progress to advanced HF, patients are faced with many challenges, such as poor quality of life due to worsening symptoms and frequent hospitalizations. Heart failure management adds significant financial burden to the health care system. Palliative care can be integrated into HF care to improve quality of life and symptom management and to address physical, spiritual, and psychosocial needs of patients and families. Palliative care can be used concurrently with or independent of curative or life-prolonging HF therapies. Transformational leadership principles were used to guide the development of a plan to enhance integration of palliative care within traditional advanced HF care.

  8. Identification of transformation products during advanced oxidation of diatrizoate: Effect of water matrix and oxidation process.

    Science.gov (United States)

    Azerrad, Sara P; Lütke Eversloh, Christian; Gilboa, Maayan; Schulz, Manoj; Ternes, Thomas; Dosoretz, Carlos G

    2016-10-15

    Removal of micropollutants from reverse osmosis (RO) brines of wastewater desalination by oxidation processes is influenced by the scavenging capacity of brines components, resulting in the accumulation of transformation products (TPs) rather than complete mineralization. In this work the iodinated contrast media diatrizoate (DTZ) was used as model compound due to its relative resistance to oxidation. Identification of TPs was performed in ultrapure water (UPW) and RO brines applying nonthermal plasma (NTP) and UVA-TiO2 as oxidation techniques. The influence of main RO brines components in the formation and accumulation of TPs, such as chloride, bicarbonate alkalinity and humic acid, was also studied during UVA-TiO2. DTZ oxidation pattern in UPW resulted similar in both UVA-TiO2 and NTP achieving 66 and 61% transformation, respectively. However, DTZ transformation in RO brines was markedly lower in UVA-TiO2 (9%) than in NTP (27%). These differences can be attributed to the synergic effect of RO brines components during NTP. Moreover, reactive species other than hydroxyl radical contributed to DTZ transformation, i.e., direct photolysis in UVA-TiO2 and direct photolysis + O3 in NTP accounted for 16 and 23%, respectively. DTZ transformation led to iodide formation in both oxidation techniques but it further oxidized to iodate by ozone in NTP. In total 14 transformation products were identified in UPW of which 3 were present only in UVA-TiO2 and 2 were present exclusively in NTP; 5 of the 14 TPs were absent in RO brines. Five of them were new and were denoted as TP-474A/B, TP-522, TP-586, TP-602, TP-628. TP-522 (mono-chlorinated) was elucidated only in presence of high chloride titer-synthetic water matrix in NTP, most probably formed by active chlorine species generated in situ. TPs accumulation in RO brines was markedly different in comparison to UPW. This denotes the influence of RO brines components in the formation of reactive species that could further attack

  9. Genetic transformation of the plant pathogens Phytophthora capsici and Phytophthora parasitica.

    OpenAIRE

    Bailey, A. M.; Mena, G L; Herrera-Estrella, L

    1991-01-01

    Phytophthora capsici and P.parasitica were transformed to hygromycin B resistance using plasmids pCM54 and pHL1, which contain the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the Ustilago maydis heat shock hsp70 gene. Enzymes Driselase and Novozyme 234 were used to generate protoplasts which were then transformed following exposure to plasmid DNA and polyethylene glycol 6000. Transformation frequencies of over 500 transformants per micrograms of DNA per ...

  10. Possible consequences of the overlap between the CaMV 35S promoter regions in plant transformation vectors used and the viral gene VI in transgenic plants.

    Science.gov (United States)

    Podevin, Nancy; du Jardin, Patrick

    2012-01-01

    Multiple variants of the Cauliflower mosaic virus 35S promoter (P35S) are used to drive the expression of transgenes in genetically modified plants, for both research purposes and commercial applications. The genetic organization of the densely packed genome of this virus results in sequence overlap between P35S and viral gene VI, encoding the multifunctional P6 protein. The present paper investigates whether introduction of P35S variants by genetic transformation is likely to result in the expression of functional domains of the P6 protein and in potential impacts in transgenic plants. A bioinformatic analysis was performed to assess the safety for human and animal health of putative translation products of gene VI overlapping P35S. No relevant similarity was identified between the putative peptides and known allergens and toxins, using different databases. From a literature study it became clear that long variants of the P35S do contain an open reading frame, when expressed, might result in unintended phenotypic changes. A flowchart is proposed to evaluate possible unintended effects in plant transformants, based on the DNA sequence actually introduced and on the plant phenotype, taking into account the known effects of ectopically expressed P6 domains in model plants.

  11. Advances in plant proteomics towards improvement of crop productivity and stress resistance

    Directory of Open Access Journals (Sweden)

    Junjie eHu

    2015-04-01

    Full Text Available Abiotic and biotic stresses constrain plant growth and development negatively impacting crop production. Plants have developed stress-specific adaptations as well as simultaneous responses to a combination of various abiotic stresses with pathogen infection. The efficiency of stress-induced adaptive responses is dependent on activation of molecular signaling pathways and intracellular networks by modulating expression, or abundance, and/or posttranslational modification of proteins primarily associated with defense mechanisms. In this review, we summarize and evaluate the contribution of proteomic studies to our understanding of stress response mechanisms in different plant organs and tissues. Advanced quantitative proteomic techniques have improved the coverage of total proteomes and sub-proteomes from small amounts of starting material, and characterized post-translational modifications as well as protein–protein interactions at the cellular level, providing detailed information on organ- and tissue-specific regulatory mechanisms responding to a variety of individual stresses or stress combinations during plant life cycle. In particular, we address the tissue-specific signaling networks localized to various organelles that participate in stress-related physiological plasticity and adaptive mechanisms, such as photosynthetic efficiency, symbiotic nitrogen fixation, plant growth, tolerance and common responses to environmental stresses. We also provide an update on the progress of proteomics with major crop species and discuss the current challenges and limitations inherent to proteomics techniques and data interpretation for non-model organisms. Future directions in proteomics research towards crop improvement are further discussed.

  12. Advances in Eco-Efficient Agriculture: The Plant-Soil Mycobiome

    Directory of Open Access Journals (Sweden)

    Marcela Claudia Pagano

    2017-02-01

    Full Text Available In order to achieve a desirable ecological and sustainable agriculture a thorough understanding of the plant-soil mycobiome is imperative. Commercial industrial agriculture alters greenhouse gas emissions, promotes loss of plant and soil biodiversity, increases pollution by raising atmospheric CO2, and releases pesticides, thus affecting both terrestrial and aquatic ecosystems. Diversified farming systems, including perennial cultivated pastures, are among worldwide strategies that aim to reduce terrestrial greenhouse gas emissions and deal with threats to global sustainability. Additionally, stimulation of soil microbes and appropriate soil management can influence soil interactions as well as the rates of organic matter decomposition and the release of gases. Agricultural soil microbial communities play a central role in ecosystem processes and are affected by biocontrol agents, biofertilizers, and exposure to pesticides, the extent to which is yet to be fully elucidated. Intercropping different plant species is beneficial, as this can increase carbon fixation by plants, transferring carbon to the soil, especially via mycorrhizas, thus modifying interplant interactions. This review focuses on agro-ecosystems, showing the latest advances in the plant-soil interface (the mycobiome for an eco-efficient agricultural production.

  13. Advancements in Root Growth Measurement Technologies and Observation Capabilities for Container-Grown Plants

    Directory of Open Access Journals (Sweden)

    Lesley A. Judd

    2015-07-01

    Full Text Available The study, characterization, observation, and quantification of plant root growth and root systems (Rhizometrics has been and remains an important area of research in all disciplines of plant science. In the horticultural industry, a large portion of the crops grown annually are grown in pot culture. Root growth is a critical component in overall plant performance during production in containers, and therefore it is important to understand the factors that influence and/or possible enhance it. Quantifying root growth has varied over the last several decades with each method of quantification changing in its reliability of measurement and variation among the results. Methods such as root drawings, pin boards, rhizotrons, and minirhizotrons initiated the aptitude to measure roots with field crops, and have been expanded to container-grown plants. However, many of the published research methods are monotonous and time-consuming. More recently, computer programs have increased in use as technology advances and measuring characteristics of root growth becomes easier. These programs are instrumental in analyzing various root growth characteristics, from root diameter and length of individual roots to branching angle and topological depth of the root architecture. This review delves into the expanding technologies involved with expertly measuring root growth of plants in containers, and the advantages and disadvantages that remain.

  14. Advanced phenotyping and phenotype data analysis for the plant growth and development study

    Directory of Open Access Journals (Sweden)

    Md. Matiur eRahaman

    2015-08-01

    Full Text Available Due to increase in the consumption of food, feed, fuel and to ensure global food security for rapidly growing human population, there is need to breed high yielding crops that can adapt to future climate. To solve these global issues, novel approaches are required to provide quantitative phenotypes to elucidate the genetic basis of agriculturally import traits and to screen germplasm with super performance in function under resource-limited environment. At present, plant phenomics has offered and integrated suite technologies for understanding the complete set of phenotypes of plants, towards the progression of the full characteristics of plants with whole sequenced genomes. In this aspect, high-throughput phenotyping platforms have been developed that enables to capture extensive and intensive phenotype data from non-destructive imaging over time. These developments advance our view on plant growth and performance with responses to the changing climate and environment. In this paper, we present a brief review on currently developed high-throughput plant phenotyping infrastructures based on imaging techniques and corresponding principles for phenotype data analysis.

  15. Strategically aligning a mandala of competencies to advance a transformative vision.

    Science.gov (United States)

    Giuse, Nunzia B; Kusnoor, Sheila V; Koonce, Taneya Y; Ryland, Christopher R; Walden, Rachel R; Naylor, Helen M; Williams, Annette M; Jerome, Rebecca N

    2013-10-01

    This paper offers insight into the processes that have shaped the Eskind Biomedical Library's (EBL's) strategic direction and its alignment to the institution's transformative vision. The academic biomedical library has a notable track record for developing and pioneering roles for information professionals focused on a sophisticated level of information provision that draws from and fuels practice evolutions. The medical center's overall transformative vision informs the creation of a fully aligned library strategic plan designed to effectively contribute to the execution of key organizational goals. Annual goals reflect organizational priorities and contain quantifiable and measurable deliverables. Two strategic themes, facilitating genetic literacy and preserving community history, are described in detail to illustrate the concept of goal setting. The strategic planning model reflects EBL's adaptation to the ever-changing needs of its organization. The paper provides a characterization of a workable model that can be replicated by other institutions.

  16. Novel recombinant binary vectors harbouring Basta (bar) gene as a plant selectable marker for genetic transformation of plants.

    Science.gov (United States)

    Nada, Reham M

    2016-04-01

    Genetic transformation is one of the most widely used technique in crop improvement. However, most of the binary vectors used in this technique, especially cloning based, contain antibiotic genes as selection marker that raise serious consumer and environmental concerns; moreover, they could be transferred to non-target hosts with deleterious effects. Therefore, the goal of this study was reconstruction of the widely used pBI121 binary vector by substituting the harmful antibiotic selection marker gene with a less-harmful selection marker, Basta (herbicide resistance gene). The generated vectors were designated as pBI121NB and pBI121CB, in which Basta gene was expressed under the control of Nos or CaMV 35S promoter, respectively. The successful integration of the new inserts into both the vectors was confirmed by PCR, restriction digestion and sequencing. Both these vectors were used in transforming Arabidopsis, Egyptian wheat and barley varieties using LBA4404 and GV3101 Agrobacterium strains. The surfactant Tween-20 resulted in an efficient transformation and the number of Arabidopsis transformants was about 6-9 %. Soaked seeds of wheat and barley were transformed with Agrobacterium to introduce the bacteria to the growing shoot apices. The percentage of transgenic lines was around 16-17 and 14-15 % for wheat and barley, respectively. The quantitative studies presented in this work showed that both LBA4404 and GV3101 strains were suitable for transforming Egyptian wheat and barley.

  17. Glancing Angle Deposition System for Advanced Fabrication of Metamaterial and Transformation-Optics Devices

    Science.gov (United States)

    2012-06-15

    CLASSIFICATION OF: Transformation optics is opening the door to new devices and applications by manipulating the flow of light on the nano-scale combining...quote received from vendor ( PVD  Products)  ­ 01/16/2011: Utility form received for pre‐installation connections  ­ 05/13/2011: Trades (plumber

  18. UNEDF: Advanced Scientific Computing Transforms the Low-Energy Nuclear Many-Body Problem

    CERN Document Server

    Stoitsov, M; Nazarewicz, W; Bulgac, A; Hagen, G; Kortelainen, M; Pei, J C; Roche, K J; Schunck, N; Thompson, I; Vary, J P; Wild, S M

    2011-01-01

    The UNEDF SciDAC collaboration of nuclear theorists, applied mathematicians, and computer scientists is developing a comprehensive description of nuclei and their reactions that delivers maximum predictive power with quantified uncertainties. This paper illustrates significant milestones accomplished by UNEDF through integration of the theoretical approaches, advanced numerical algorithms, and leadership class computational resources.

  19. The Transformation of Learning: Advances in Cultural-Historical Activity Theory

    Science.gov (United States)

    van Oers, Bert, Ed.; Wardekker, Wim, Ed.; Elbers, Ed, Ed.; van der Veer, Rene, Ed.

    2010-01-01

    Learning is a changing phenomenon, depending on the advances in theory and research. This book presents a relatively new approach to learning, based on meaningful human activities in cultural practices and in collaboration with others. It draws extensively from the ideas of Lev Vygotsky and his recent followers. The book presents ideas that…

  20. Introduction of soft X-ray spectromicroscopy as an advanced technique for plant biopolymers research.

    Directory of Open Access Journals (Sweden)

    Chithra Karunakaran

    Full Text Available Soft X-ray absorption spectroscopy coupled with nano-scale microscopy has been widely used in material science, environmental science, and physical sciences. In this work, the advantages of soft X-ray absorption spectromicroscopy for plant biopolymer research were demonstrated by determining the chemical sensitivity of the technique to identify common plant biopolymers and to map the distributions of biopolymers in plant samples. The chemical sensitivity of soft X-ray spectroscopy to study biopolymers was determined by recording the spectra of common plant biopolymers using soft X-ray and Fourier Transform mid Infrared (FT-IR spectroscopy techniques. The soft X-ray spectra of lignin, cellulose, and polygalacturonic acid have distinct spectral features. However, there were no distinct differences between cellulose and hemicellulose spectra. Mid infrared spectra of all biopolymers were unique and there were differences between the spectra of water soluble and insoluble xylans. The advantage of nano-scale spatial resolution exploited using soft X-ray spectromicroscopy for plant biopolymer research was demonstrated by mapping plant cell wall biopolymers in a lentil stem section and compared with the FT-IR spectromicroscopy data from the same sample. The soft X-ray spectromicroscopy enables mapping of biopolymers at the sub-cellular (~30 nm resolution whereas, the limited spatial resolution in the micron scale range in the FT-IR spectromicroscopy made it difficult to identify the localized distribution of biopolymers. The advantages and limitations of soft X-ray and FT-IR spectromicroscopy techniques for biopolymer research are also discussed.

  1. Introduction of soft X-ray spectromicroscopy as an advanced technique for plant biopolymers research.

    Science.gov (United States)

    Karunakaran, Chithra; Christensen, Colleen R; Gaillard, Cedric; Lahlali, Rachid; Blair, Lisa M; Perumal, Vijayan; Miller, Shea S; Hitchcock, Adam P

    2015-01-01

    Soft X-ray absorption spectroscopy coupled with nano-scale microscopy has been widely used in material science, environmental science, and physical sciences. In this work, the advantages of soft X-ray absorption spectromicroscopy for plant biopolymer research were demonstrated by determining the chemical sensitivity of the technique to identify common plant biopolymers and to map the distributions of biopolymers in plant samples. The chemical sensitivity of soft X-ray spectroscopy to study biopolymers was determined by recording the spectra of common plant biopolymers using soft X-ray and Fourier Transform mid Infrared (FT-IR) spectroscopy techniques. The soft X-ray spectra of lignin, cellulose, and polygalacturonic acid have distinct spectral features. However, there were no distinct differences between cellulose and hemicellulose spectra. Mid infrared spectra of all biopolymers were unique and there were differences between the spectra of water soluble and insoluble xylans. The advantage of nano-scale spatial resolution exploited using soft X-ray spectromicroscopy for plant biopolymer research was demonstrated by mapping plant cell wall biopolymers in a lentil stem section and compared with the FT-IR spectromicroscopy data from the same sample. The soft X-ray spectromicroscopy enables mapping of biopolymers at the sub-cellular (~30 nm) resolution whereas, the limited spatial resolution in the micron scale range in the FT-IR spectromicroscopy made it difficult to identify the localized distribution of biopolymers. The advantages and limitations of soft X-ray and FT-IR spectromicroscopy techniques for biopolymer research are also discussed.

  2. Introduction of Soft X-Ray Spectromicroscopy as an Advanced Technique for Plant Biopolymers Research

    Science.gov (United States)

    Karunakaran, Chithra; Christensen, Colleen R.; Gaillard, Cedric; Lahlali, Rachid; Blair, Lisa M.; Perumal, Vijayan; Miller, Shea S.; Hitchcock, Adam P.

    2015-01-01

    Soft X-ray absorption spectroscopy coupled with nano-scale microscopy has been widely used in material science, environmental science, and physical sciences. In this work, the advantages of soft X-ray absorption spectromicroscopy for plant biopolymer research were demonstrated by determining the chemical sensitivity of the technique to identify common plant biopolymers and to map the distributions of biopolymers in plant samples. The chemical sensitivity of soft X-ray spectroscopy to study biopolymers was determined by recording the spectra of common plant biopolymers using soft X-ray and Fourier Transform mid Infrared (FT-IR) spectroscopy techniques. The soft X-ray spectra of lignin, cellulose, and polygalacturonic acid have distinct spectral features. However, there were no distinct differences between cellulose and hemicellulose spectra. Mid infrared spectra of all biopolymers were unique and there were differences between the spectra of water soluble and insoluble xylans. The advantage of nano-scale spatial resolution exploited using soft X-ray spectromicroscopy for plant biopolymer research was demonstrated by mapping plant cell wall biopolymers in a lentil stem section and compared with the FT-IR spectromicroscopy data from the same sample. The soft X-ray spectromicroscopy enables mapping of biopolymers at the sub-cellular (~30 nm) resolution whereas, the limited spatial resolution in the micron scale range in the FT-IR spectromicroscopy made it difficult to identify the localized distribution of biopolymers. The advantages and limitations of soft X-ray and FT-IR spectromicroscopy techniques for biopolymer research are also discussed. PMID:25811457

  3. Advancement of Phenotype Transformation of Cancer-associated Fibroblasts: 
from Genetic Alterations to Epigenetic Modification

    Directory of Open Access Journals (Sweden)

    Dali CHEN

    2015-02-01

    Full Text Available In the field of human cancer research, even though the vast majority attentions were paid to tumor cells as “the seeds”, the roles of tumor microenvironments as “the soil” are gradually explored in recent years. As a dominant compartment of tumor microenvironments, cancer-associated fibroblasts (CAFs were discovered to correlated with tumorigenesis, tumor progression and prognosis. And the exploration of the mechanisms of CAF phenotype transformation would conducive to the further understand of the CAFs function in human cancers. As we known that CAFs have four main origins, including epithelial cells, endothelial cells, mesenchymal stem cells (MSCs and local mesenchymal cells. However, researchers found that all these origins finally conduct similiar phenotypes from intrinsic to extrinsic ones. Thus, what and how a mechanism can conduct the phenotype transformation of CAFs with different origins? Two viewpoints are proposed to try to answer the quetsion, involving genetic alterations and epigenetic modifications. This review will systematically summarize the advancement of mechanisms of CAF phenotype transformations in the aspect of genentic and epigenetic modifications.

  4. The Physics Basis For An Advanced Physics And Advanced Technology Tokamak Power Plant Configuration, ARIES-ACT1

    Energy Technology Data Exchange (ETDEWEB)

    Charles Kessel, et al

    2014-03-05

    The advanced physics and advanced technology tokamak power plant ARIES-ACT1 has a major radius of 6.25 m at aspect ratio of 4.0, toroidal field of 6.0 T, strong shaping with elongation of 2.2 and triangularity of 0.63. The broadest pressure cases reached wall stabilized βN ~ 5.75, limited by n=3 external kink mode requiring a conducting shell at b/a = 0.3, and requiring plasma rotation, feedback, and or kinetic stabilization. The medium pressure peaking case reached βN = 5.28 with BT = 6.75, while the peaked pressure case reaches βN < 5.15. Fast particle MHD stability shows that the alpha particles are unstable, but this leads to redistribution to larger minor radius rather than loss from the plasma. Edge and divertor plasma modeling show that about 75% of the power to the divertor can be radiated with an ITER-like divertor geometry, while over 95% can be radiated in a stable detached mode with an orthogonal target and wide slot geometry. The bootstrap current fraction is 91% with a q95 of 4.5, requiring about ~ 1.1 MA of external current drive. This current is supplied with 5 MW of ICRF/FW and 40 MW of LHCD. EC was examined and is most effective for safety factor control over ρ ~ 0.2-0.6 with 20 MW. The pedestal density is ~ 0.9x1020 /m3 and the temperature is ~ 4.4 keV. The H98 factor is 1.65, n/nGr = 1.0, and the net power to LH threshold power is 2.8- 3.0 in the flattop.

  5. An advanced field experimental design to assess plant tolerance to heavy metal pollution

    Science.gov (United States)

    Łopata, Barbara; Szarek-Łukaszewska, Grażyna; Babst-Kostecka, Alicja

    2016-04-01

    Only a limited number of vascular plant species can survive and reproduce in toxic metalliferous environments. Among these species, pseudometallophytes are particularly interesting, as their metallicolous (M) populations on metalliferous soils and non-metallicolous (NM) populations on non-metalliferous soils show very pronounced ecological differences. Pseudometallophytes thus provide excellent opportunities for multidisciplinary research to improve phytoremediation and phytomining. Numerous methods have been developed to investigate plant adaptation to metal pollution, the majority of which has been conducted under controlled laboratory conditions. Although these efforts have significantly advanced our understanding of mechanisms underlying metal tolerance in plants, populations must be reciprocally transplanted to clearly identify natural selection. Only then is it possible to test, whether the fitness of native plants is higher than that of nonnative populations and thereby prove local adaptation. Here, we present an enhanced field experimental design aimed at verification of local adaptation to habitats with different levels of heavy metal soil contamination. At two M and two NM sites, we established a total of 12 plots (4 sites x 3 plots each), removed the existing local vegetation, and collected soil samples for chemical analyses (5 samples per plot). Plant collection (N= 480) from all four selected populations was established under laboratory conditions prior to the transplant experiment. Genotypes were randomly distributed within each plot (240 x 270 cm) and planted along a regulary spaced grid (30x30cm cell size) in spring 2015. Measurements will start in spring 2016, by which time plants are expected to have acclimatized to the local conditions. For the two subsiquent years, growth, survival, fitness, life cycle and herbivory consumption will be monitored for each transplant. On a weekly basis, we will record: 1) pictures of each transplant to determine

  6. 氯碱厂节水技术改造%Water saving technology transformation of chlor-alkali plant

    Institute of Scientific and Technical Information of China (English)

    董雷; 刘勇先

    2012-01-01

    介绍了氯碱厂一次盐水、电解、氯气处理、氢气处理工序的节水改造情况。通过技术改造,回收了大量的冷凝水及废水,减少了酸性水对厂房、设备的腐蚀。%The water saving technology transformation of chlor-alkali plant was introduced,including the brine process,electrolysis process and chlorine and hydrogen treatment.After technology transformation,the waste water was recycled,and reduced the corrosion of acidic water for the plant and equipment.The economic and social benefits were better.

  7. Generation of marker- and/or backbone-free transgenic wheat plants via Agrobacterium-mediated transformation

    Directory of Open Access Journals (Sweden)

    Wang Genping

    2016-09-01

    Full Text Available Horizontal transfer of antibiotic resistance genes to animals and vertical transfer of herbicide resistance genes to the weedy relatives are perceived as major biosafety concerns in genetically modified (GM crops. In this study, five novel vectors which used gusA and bar as a reporter gene and a selection marker gene, respectively, were constructed based on the pCLEAN dual binary vector system. Among these vectors, 1G7B and 5G7B carried two T-DNAs located on two respective plasmids with 5G7B possessing an additional virGwt gene. 5LBTG154 and 5TGTB154 carried two T-DNAs in the target plasmid with either one or double right borders, and 5BTG154 carried the selectable marker gene on the backbone outside of the T-DNA left border in the target plasmid. In addition, 5BTG154, 5LBTG154 and 5TGTB154 used pAL154 as a helper plasmid which contains Komari fragment to facilitate transformation. These five dual binary vector combinations were transformed into Agrobacterium strain AGL1 and used to transform durum wheat cv Stewart 63. Evaluation of the co-transformation efficiencies, the frequencies of marker-free transgenic plants and integration of backbone sequences in the obtained transgenic lines indicated that two vectors (5G7B and 5TGTB154 were more efficient in generating marker-free transgenic wheat plants with no or minimal integration of backbone sequences in the wheat genome. The vector series developed in this study for generation of marker- and/or backbone-free transgenic wheat plants via Agrobacterium-mediated transformation will be useful to facilitate the creation of ‘clean’ GM wheat containing only the foreign genes of agronomic importance.

  8. Generation of Marker- and/or Backbone-Free Transgenic Wheat Plants via Agrobacterium-Mediated Transformation

    Science.gov (United States)

    Wang, Gen-Ping; Yu, Xiu-Dao; Sun, Yong-Wei; Jones, Huw D.; Xia, Lan-Qin

    2016-01-01

    Horizontal transfer of antibiotic resistance genes to animals and vertical transfer of herbicide resistance genes to the weedy relatives are perceived as major biosafety concerns in genetically modified (GM) crops. In this study, five novel vectors which used gusA and bar as a reporter gene and a selection marker gene, respectively, were constructed based on the pCLEAN dual binary vector system. Among these vectors, 1G7B and 5G7B carried two T-DNAs located on two respective plasmids with 5G7B possessing an additional virGwt gene. 5LBTG154 and 5TGTB154 carried two T-DNAs in the target plasmid with either one or double right borders, and 5BTG154 carried the selectable marker gene on the backbone outside of the T-DNA left border in the target plasmid. In addition, 5BTG154, 5LBTG154, and 5TGTB154 used pAL154 as a helper plasmid which contains Komari fragment to facilitate transformation. These five dual binary vector combinations were transformed into Agrobacterium strain AGL1 and used to transform durum wheat cv Stewart 63. Evaluation of the co-transformation efficiencies, the frequencies of marker-free transgenic plants, and integration of backbone sequences in the obtained transgenic lines indicated that two vectors (5G7B and 5TGTB154) were more efficient in generating marker-free transgenic wheat plants with no or minimal integration of backbone sequences in the wheat genome. The vector series developed in this study for generation of marker- and/or backbone-free transgenic wheat plants via Agrobacterium-mediated transformation will be useful to facilitate the creation of “clean” GM wheat containing only the foreign genes of agronomic importance. PMID:27708648

  9. A Revolution in the Making: Advances in Materials That May Transform Future Exploration Infrastructures and Missions

    Science.gov (United States)

    Harris, Charles E.; Dicus, Dennis L.; Shuart, Mark J.

    2001-01-01

    The NASA Strategic Plan identifies the long-term goal to provide safe and affordable space access, orbital transfer, and interplanetary transportation capabilities to enable research, human exploration, and the commercial development of space; and to conduct human and robotic missions to planets and other bodies in our solar system. Numerous scientific and engineering breakthroughs will be required to develop the technology necessary to achieve this goal. Critical technologies include advanced vehicle primary and secondary structure, radiation protection, propulsion and power systems, fuel storage, electronics and devices, sensors and science instruments, and medical diagnostics and treatment. Advanced materials with revolutionary new capabilities are an essential element of each of these technologies. This paper discusses those materials best suited for aerospace vehicle structure and highlights the enormous potential of one revolutionary new material, carbon nanotubes.

  10. UNEDF: Advanced Scientific Computing Collaboration Transforms the Low-Energy Nuclear Many-Body Problem

    CERN Document Server

    Nam, H; Nazarewicz, W; Bulgac, A; Hagen, G; Kortelainen, M; Maris, P; Pei, J C; Roche, K J; Schunck, N; Thompson, I; Vary, J P; Wild, S M

    2012-01-01

    The demands of cutting-edge science are driving the need for larger and faster computing resources. With the rapidly growing scale of computing systems and the prospect of technologically disruptive architectures to meet these needs, scientists face the challenge of effectively using complex computational resources to advance scientific discovery. Multidisciplinary collaborating networks of researchers with diverse scientific backgrounds are needed to address these complex challenges. The UNEDF SciDAC collaboration of nuclear theorists, applied mathematicians, and computer scientists is developing a comprehensive description of nuclei and their reactions that delivers maximum predictive power with quantified uncertainties. This paper describes UNEDF and identifies attributes that classify it as a successful computational collaboration. We illustrate significant milestones accomplished by UNEDF through integrative solutions using the most reliable theoretical approaches, most advanced algorithms, and leadershi...

  11. A Revolution in the Making: Advances in Materials That May Transform Future Exploration Infrastructures and Missions

    Science.gov (United States)

    Harris, Charles E.; Dicus, Dennis L.; Shuart, Mark J.

    2001-01-01

    The NASA Strategic Plan identifies the long-term goal to provide safe and affordable space access, orbital transfer, and interplanetary transportation capabilities to enable research, human exploration, and the commercial development of space; and to conduct human and robotic missions to planets and other bodies in our solar system. Numerous scientific and engineering breakthroughs will be required to develop the technology necessary to achieve this goal. Critical technologies include advanced vehicle primary and secondary structure, radiation protection, propulsion and power systems, fuel storage, electronics and devices, sensors and science instruments, and medical diagnostics and treatment. Advanced materials with revolutionary new capabilities are an essential element of each of these technologies. This paper discusses those materials best suited for aerospace vehicle structure and highlights the enormous potential of one revolutionary new material, carbon nanotubes.

  12. Agrobacterium tumefaciens-mediated transformation of embryogenic tissue and transgenic plant regeneration in Chamaecyparis obtusa Sieb. et Zucc.

    Science.gov (United States)

    Taniguchi, T; Kurita, M; Ohmiya, Y; Kondo, T

    2005-03-01

    A genetic transformation procedure for Chamaecyparis obtusa was developed after co-cultivation of embryogenic tissues with disarmed Agrobacterium tumefaciens strain C58/pMP90, which harbours the sgfp (synthetic green fluorescent protein) visual reporter and nptII (neomycin phoshotransferase II) selectable marker genes. The highest transformation frequency was 22.5 independent transformed lines per dish (250 mg embryogenic tissue) following selection on kanamycin medium. Transgenic plantlets were regenerated through the maturation and germination of somatic embryos. The intensity of GFP fluorescence, observed under a fluorescence microscope, varied from very faint to relatively strong, depending on the transgenic line or part of the transgenic plant. The integration of the genes into the genome of regenerated plantlets was confirmed by Southern blot analysis.

  13. Plant Regeneration from Immature Inflorescence Culture and Genetic Transformation of Wheatgrass (Agropyron cristatum × A. Desertorum cv. Hycrest-Mengnong)

    Institute of Scientific and Technical Information of China (English)

    HUO Xiu-wen; WEI Jian-hua; XU Chun-bo; MI Fu-gui; YUN Jin-feng

    2006-01-01

    The plants of hybrid wheatgrass (A. Cristatum ×A. Desertorum cv. Hycrest-Mengnong) were directly induced from embryogenic callus regenerated from immature inflorescence. Immature inflorescence was cultured on improved MS medium containing 2.0-3.0 mg L-1 2,4-D to regenerate callus. The calli were then transferred to hormone-free MS medium for differentiation and 1/2 MS medium for rooting. Results showed that callus initiation frequency was 83.4% and plant regeneration frequency was 59.6%. Phosphinothricin acetyltransferase (bar) gene was transformed into the hybrid wheatgrass by particle bombardment. Resistant callus was obtained using selecting agent, herbicide glufosinate of 0.5 mg L-1, and some transgenic plants were recovered in vitro. The transgenic plants were identified by PCR and Southern blot analysis and these plants developed normally in the glufosinate medium, whereas the nontransgenic plants did not. The results demonstrated that bar cDNA integrated into the genomic DNA of the transgenic plants. The transgenic frequencies of bar gene were 1.1%.

  14. Advances in processing technologies for titanium heat exchanger tubes of fossil and nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Likhareva, T.P.; Tchizhik, A.A.; Chavchanidze, N.N. [Polzanov Central Boiler and Turbine Institute, St. Petersburg (Russian Federation)

    1998-12-31

    The advances in processing technologies for titanium heat exchangers with rolled and welded tubes of fossil and nuclear power plants in Russia are presented. The special methodology of investigations with constant small strain rate have been used to study the effects of mixed corrosion and creep processes in condensers cooled by sea or synthetic sea waters. The results of corrosion creep tests and K1scc calculations are given. The Russian science activities concerning condensers manufactured from titanium show the possibilities for designing structures with very high level service reliability in different corrosion aggressive mediums with high total salt, Cl-ion and oxygen contents. (orig.)

  15. Advances in the Plant Isoprenoid Biosynthesis Pathway and Its Metabolic Engineering

    Institute of Scientific and Technical Information of China (English)

    Yan LIU; Hong WANG; He-Chun YE; Guo-Feng LI

    2005-01-01

    Although the cytosolic isoprenoid biosynthetic pathway, mavolonate pathway, in plants has been known for many years, a new plastidial 1-deoxyxylulose-5-phosphate (DXP) pathway was identified in the past few years and its related intermediates, enzymes, and genes have been characterized quite recently.With a deep insight into the biosynthetic pathway of isoprenoids, investigations into the metabolic engineering of isoprenoid biosynthesis have started to prosper. In the present article, recent advances in the discoveries and regulatory roles of new genes and enzymes in the plastidial isoprenoid biosynthesis path way are reviewed and examples of the metabolic engineering of cytosolic and plastidial isoprenoids biosnthesis are discussed.

  16. High production of plant type levan in sugar beet transformed with timothy (Phleum pratense) 6-SFT genes.

    Science.gov (United States)

    Matsuhira, Hiroaki; Tamura, Ken-ichi; Tamagake, Hideto; Sato, Yutaka; Anzai, Hiroyuki; Yoshida, Midori

    2014-12-20

    Levan, a type of fructan, is an oligomer or polymer with mainly a β(2,6)-linked fructose chain attached to sucrose. We introduced two timothy genes, PpFT1 and PpFT2, coding for two homologous sucrose:fructan 6-fructosyltransferases into sugar beet. Sugar beet produces a high concentration of sucrose, a starting substrate in fructan synthesis, in the root. Among transgenic T1 lines, we obtained sugar beet transformants that accumulated large amounts of β(2,6)-linked levans (about 20 to 75mgg(-1) FW) in the roots. The transformed sugar beet plants possessing PpFT1 or PpFT2 produced linear levans with different degrees of polymerization (DP). Namely, the PpFT1 transformants accumulated mainly high DP levans including those with DP>40, while the PpFT2 transformants accumulated levans with DP between 3 and 40. Chromatograms showed that PpFT2 produces pure β(2,6)-linked linear levans compared with fructans synthesized by PpFT1. These levans belong to the high DP class of plant fructans, but have much shorter DP than that of levans generally produced by microorganisms.

  17. Proposing a new advanced control technique for micro hydro power plants

    Energy Technology Data Exchange (ETDEWEB)

    Hanmandlu, M.; Goyal, Himani [Department of Electrical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi 110016 (India)

    2008-05-15

    Micro hydropower plants are emerging as a major renewable energy resource today as they do not encounter the problems of population displacement and environmental problems associated with the large hydro power plants. However, they require control systems to limit the huge variation in input flows expected in rivulets over which these are established to produce a constant power supply. This paper proposes an electric servomotor as a governor for a micro hydro power plant especially those plants that are operated in isolated mode. An advanced controller is developed combining four control schemes for the control of the governor following the concept that the control action can be split up into linear and non-linear parts. The linear part of this controller contains an adaptive fast transversal filter (FTF) algorithm and normalized LMS (nLMS) algorithm. The non-linear part of the controller incorporates Fuzzy PI and a neural network. The concept behind splitting the control action is reasoned out and the conditions for stability of the controller are proved. The new controller has a superior performance compared to other control schemes. (author)

  18. In planta Transformed Cumin (Cuminum cyminum L. Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance.

    Directory of Open Access Journals (Sweden)

    Sonika Pandey

    Full Text Available Cumin is an annual, herbaceous, medicinal, aromatic, spice glycophyte that contains diverse applications as a food and flavoring additive, and therapeutic agents. An efficient, less time consuming, Agrobacterium-mediated, a tissue culture-independent in planta genetic transformation method was established for the first time using cumin seeds. The SbNHX1 gene, cloned from an extreme halophyte Salicornia brachiata was transformed in cumin using optimized in planta transformation method. The SbNHX1 gene encodes a vacuolar Na+/H+ antiporter and is involved in the compartmentalization of excess Na+ ions into the vacuole and maintenance of ion homeostasis Transgenic cumin plants were confirmed by PCR using gene (SbNHX1, uidA and hptII specific primers. The single gene integration event and overexpression of the gene were confirmed by Southern hybridization and competitive RT-PCR, respectively. Transgenic lines L3 and L13 showed high expression of the SbNHX1 gene compared to L6 whereas moderate expression was detected in L5 and L10 transgenic lines. Transgenic lines (L3, L5, L10 and L13, overexpressing the SbNHX1 gene, showed higher photosynthetic pigments (chlorophyll a, b and carotenoid, and lower electrolytic leakage, lipid peroxidation (MDA content and proline content as compared to wild type plants under salinity stress. Though transgenic lines were also affected by salinity stress but performed better compared to WT plants. The ectopic expression of the SbNHX1 gene confirmed enhanced salinity stress tolerance in cumin as compared to wild type plants under stress condition. The present study is the first report of engineering salt tolerance in cumin, so far and the plant may be utilized for the cultivation in saline areas.

  19. In planta Transformed Cumin (Cuminum cyminum L.) Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance.

    Science.gov (United States)

    Pandey, Sonika; Patel, Manish Kumar; Mishra, Avinash; Jha, Bhavanath

    2016-01-01

    Cumin is an annual, herbaceous, medicinal, aromatic, spice glycophyte that contains diverse applications as a food and flavoring additive, and therapeutic agents. An efficient, less time consuming, Agrobacterium-mediated, a tissue culture-independent in planta genetic transformation method was established for the first time using cumin seeds. The SbNHX1 gene, cloned from an extreme halophyte Salicornia brachiata was transformed in cumin using optimized in planta transformation method. The SbNHX1 gene encodes a vacuolar Na+/H+ antiporter and is involved in the compartmentalization of excess Na+ ions into the vacuole and maintenance of ion homeostasis Transgenic cumin plants were confirmed by PCR using gene (SbNHX1, uidA and hptII) specific primers. The single gene integration event and overexpression of the gene were confirmed by Southern hybridization and competitive RT-PCR, respectively. Transgenic lines L3 and L13 showed high expression of the SbNHX1 gene compared to L6 whereas moderate expression was detected in L5 and L10 transgenic lines. Transgenic lines (L3, L5, L10 and L13), overexpressing the SbNHX1 gene, showed higher photosynthetic pigments (chlorophyll a, b and carotenoid), and lower electrolytic leakage, lipid peroxidation (MDA content) and proline content as compared to wild type plants under salinity stress. Though transgenic lines were also affected by salinity stress but performed better compared to WT plants. The ectopic expression of the SbNHX1 gene confirmed enhanced salinity stress tolerance in cumin as compared to wild type plants under stress condition. The present study is the first report of engineering salt tolerance in cumin, so far and the plant may be utilized for the cultivation in saline areas.

  20. Recent advances in development of marker-free transgenic plants: Regulation and biosafety concern

    Indian Academy of Sciences (India)

    Narendra Tuteja; Shiv Verma; Ranjan Kumar Sahoo; Sebastian Raveendar; In Bheema Lingeshwara Reddy

    2012-03-01

    During the efficient genetic transformation of plants with the gene of interest, some selectable marker genes are also used in order to identify the transgenic plant cells or tissues. Usually, antibiotic- or herbicide-selective agents and their corresponding resistance genes are used to introduce economically valuable genes into crop plants. From the biosafety authority and consumer viewpoints, the presence of selectable marker genes in released transgenic crops may be transferred to weeds or pathogenic microorganisms in the gastrointestinal tract or soil, making them resistant to treatment with herbicides or antibiotics, respectively. Sexual crossing also raises the problem of transgene expression because redundancy of transgenes in the genome may trigger homology-dependent gene silencing. The future potential of transgenic technologies for crop improvement depends greatly on our abilities to engineer stable expression of multiple transgenic traits in a predictable fashion and to prevent the transfer of undesirable transgenic material to non-transgenic crops and related species. Therefore, it is now essential to develop an efficient marker-free transgenic system. These considerations underline the development of various approaches designed to facilitate timely elimination of transgenes when their function is no longer needed. Due to the limiting number of available selectable marker genes, in future the stacking of transgenes will be increasingly desirable. The production of marker-free transgenic plants is now a critical requisite for their commercial deployment and also for engineering multiple and complex trait. Here we describe the current technologies to eliminate the selectablemarker genes (SMG) in order to develop marker-free transgenic plants and also discuss the regulation and biosafety concern of genetically modified (GM) crops.

  1. Advanced virtual energy simulation training and research: IGCC with CO2 capture power plant

    Energy Technology Data Exchange (ETDEWEB)

    Zitney, S.; Liese, E.; Mahapatra, P.; Bhattacharyya, D.; Provost, G.

    2011-01-01

    In this presentation, we highlight the deployment of a real-time dynamic simulator of an integrated gasification combined cycle (IGCC) power plant with CO{sub 2} capture at the Department of Energy's (DOE) National Energy Technology Laboratory's (NETL) Advanced Virtual Energy Simulation Training and Research (AVESTARTM) Center. The Center was established as part of the DOE's accelerating initiative to advance new clean coal technology for power generation. IGCC systems are an attractive technology option, generating low-cost electricity by converting coal and/or other fuels into a clean synthesis gas mixture in a process that is efficient and environmentally superior to conventional power plants. The IGCC dynamic simulator builds on, and reaches beyond, conventional power plant simulators to merge, for the first time, a 'gasification with CO{sub 2} capture' process simulator with a 'combined-cycle' power simulator. Fueled with coal, petroleum coke, and/or biomass, the gasification island of the simulated IGCC plant consists of two oxygen-blown, downward-fired, entrained-flow, slagging gasifiers with radiant syngas coolers and two-stage sour shift reactors, followed by a dual-stage acid gas removal process for CO{sub 2} capture. The combined cycle island consists of two F-class gas turbines, steam turbine, and a heat recovery steam generator with three-pressure levels. The dynamic simulator can be used for normal base-load operation, as well as plant start-up and shut down. The real-time dynamic simulator also responds satisfactorily to process disturbances, feedstock blending and switchovers, fluctuations in ambient conditions, and power demand load shedding. In addition, the full-scope simulator handles a wide range of abnormal situations, including equipment malfunctions and failures, together with changes initiated through actions from plant field operators. By providing a comprehensive IGCC operator training system, the

  2. Im"plant"ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation.

    Science.gov (United States)

    Kajiura, Hiroyuki; Fujiyama, Kazuhito

    2015-01-01

    Enzymatic activity assay of exogenous glycosyltransferase (GT) and glycosylhydrolase (GH) expressed in plants is an important analysis for determination of the expression of the gene of interest. However, generations and establishment of in planta transgenic lines are time-consuming. Furthermore, the expression levels and the activities of the exogenous GTs and GHs are quite low and weak, the radiolabeled donor substrate had to be used to analyze the enzymatic activity. Here, we describe a protocol for the generation of transgenic plants using suspension-cultured cells and a high sensitive assay for GT, especially β1,4-galactosyltransferase, using microsomal fraction from plant cells and fluorescent-labeled sugar chains as an acceptor substrate. This method enables less-time-consuming preparation of stable transgenic plants, non-radiolabeled, high-throughput detail analysis which includes mass spectrometric analysis and exo-glycosidase digestions.

  3. Advances in biotechnology and linking outputs to variation in complex traits: Plant and Animal Genome meeting January 2012.

    Science.gov (United States)

    Appels, R; Barrero, R; Bellgard, M

    2012-03-01

    The Plant and Animal Genome (PAG, held annually) meeting in January 2012 provided insights into the advances in plant, animal, and microbe genome studies particularly as they impact on our understanding of complex biological systems. The diverse areas of biology covered included the advances in technologies, variation in complex traits, genome change in evolution, and targeting phenotypic changes, across the broad spectrum of life forms. This overview aims to summarize the major advances in research areas presented in the plenary lectures and does not attempt to summarize the diverse research activities covered throughout the PAG in workshops, posters, presentations, and displays by suppliers of cutting-edge technologies.

  4. Promising Pedagogy: Advancing the Educational Experience of Queer Students Through Transformative Leadership.

    Science.gov (United States)

    Zook, Thomas

    2016-12-05

    The school experience for queer youth is often quite different from that of their heterosexual and gender-conforming peers; yet it is often the c ase that little attention is given to the disparate and inequitable educational and social conditions under which lesbian, gay, bisexual, transgender, and queer/questioning (LGBTQ) youth must learn. In fact, the entirety of pedagogical structures in many schools creates milieus where queer youth, those who have loved ones who are queer, and those who are merely perceived to be queer are systematically marginalized and deprived of their right to a safe, supportive, and equitable educational experience. Transformative leadership theory (TLT) inspires educational leaders to create inclusive and excellent schools for all youth. Neither a prescriptive model nor a process-oriented theory, TLT offers eight tenets that operate in concert to form an authentic way of being informing and guiding leaders' decisions and actions toward the goal of individual, organizational, and societal change.

  5. Agrobacterium-Mediated Transformation of Embryogenic Calli of Anliucheng and Regeneration of Plants Containing the Chimaeric Ribonuclease Gene

    Institute of Scientific and Technical Information of China (English)

    LI Dong-dong; SHI Wei; DENG Xiu-xin

    2003-01-01

    Anliucheng (Citrus sinensis Osbeck), a very seedy and widely spread acidless sweet orange cultivar in south of China, was transformed by the strain of Agrobacterium Tumefaciens EHA105 carrying pTA29-barnase gene, which will induce pollen sterility in transgenic plants. The embryogenic calli of Anliucheng were co-cultivated with Agrobacterium tumefaciens for 3 days, and then transferred to selective medium containing 50 mg L-1 basta (a kind of herbicide) for 5 weeks. The resistant calli were recovered and regenerated 118 embryoids. A total of 13 entire plants were obtained after micro-grafted on trifoliate orange. These regenerated plants were verified by PCR amplification and confirmed by PCR-Southern blotting analysis.

  6. Advances in the Glass Formulations for the Hanford Tank Waste Treatment and Immobilization Plant

    Energy Technology Data Exchange (ETDEWEB)

    Kruger, Albert A.; Vienna, John D.; Kim, Dong Sang

    2015-01-14

    The Department of Energy-Office of River Protection (DOE-ORP) is constructing the Hanford Tank Waste Treatment and Immobilization Plant (WTP) to treat radioactive waste currently stored in underground tanks at the Hanford site in Washington. The WTP that is being designed and constructed by a team led by Bechtel National, Inc. (BNI) will separate the tank waste into High Level Waste (HLW) and Low Activity Waste (LAW) fractions with the majority of the mass (~90%) directed to LAW and most of the activity (>95%) directed to HLW. The pretreatment process, envisioned in the baseline, involves the dissolution of aluminum-bearing solids so as to allow the aluminum salts to be processed through the cesium ion exchange and report to the LAW Facility. There is an oxidative leaching process to affect a similar outcome for chromium-bearing wastes. Both of these unit operations were advanced to accommodate shortcomings in glass formulation for HLW inventories. A by-product of this are a series of technical challenges placed upon materials selected for the processing vessels. The advances in glass formulation play a role in revisiting the flow sheet for the WTP and hence, the unit operations that were being imposed by minimal waste loading requirements set forth in the contract for the design and construction of the plant. Another significant consideration to the most recent revision of the glass models are the impacts on resolution of technical questions associated with current efforts for design completion.

  7. FPGA-Based Smart Sensor for Drought Stress Detection in Tomato Plants Using Novel Physiological Variables and Discrete Wavelet Transform

    Directory of Open Access Journals (Sweden)

    Carlos Duarte-Galvan

    2014-10-01

    Full Text Available Soil drought represents one of the most dangerous stresses for plants. It impacts the yield and quality of crops, and if it remains undetected for a long time, the entire crop could be lost. However, for some plants a certain amount of drought stress improves specific characteristics. In such cases, a device capable of detecting and quantifying the impact of drought stress in plants is desirable. This article focuses on testing if the monitoring of physiological process through a gas exchange methodology provides enough information to detect drought stress conditions in plants. The experiment consists of using a set of smart sensors based on Field Programmable Gate Arrays (FPGAs to monitor a group of plants under controlled drought conditions. The main objective was to use different digital signal processing techniques such as the Discrete Wavelet Transform (DWT to explore the response of plant physiological processes to drought. Also, an index-based methodology was utilized to compensate the spatial variation inside the greenhouse. As a result, differences between treatments were determined to be independent of climate variations inside the greenhouse. Finally, after using the DWT as digital filter, results demonstrated that the proposed system is capable to reject high frequency noise and to detect drought conditions.

  8. FPGA-based smart sensor for drought stress detection in tomato plants using novel physiological variables and discrete wavelet transform.

    Science.gov (United States)

    Duarte-Galvan, Carlos; Romero-Troncoso, Rene de J; Torres-Pacheco, Irineo; Guevara-Gonzalez, Ramon G; Fernandez-Jaramillo, Arturo A; Contreras-Medina, Luis M; Carrillo-Serrano, Roberto V; Millan-Almaraz, Jesus R

    2014-10-09

    Soil drought represents one of the most dangerous stresses for plants. It impacts the yield and quality of crops, and if it remains undetected for a long time, the entire crop could be lost. However, for some plants a certain amount of drought stress improves specific characteristics. In such cases, a device capable of detecting and quantifying the impact of drought stress in plants is desirable. This article focuses on testing if the monitoring of physiological process through a gas exchange methodology provides enough information to detect drought stress conditions in plants. The experiment consists of using a set of smart sensors based on Field Programmable Gate Arrays (FPGAs) to monitor a group of plants under controlled drought conditions. The main objective was to use different digital signal processing techniques such as the Discrete Wavelet Transform (DWT) to explore the response of plant physiological processes to drought. Also, an index-based methodology was utilized to compensate the spatial variation inside the greenhouse. As a result, differences between treatments were determined to be independent of climate variations inside the greenhouse. Finally, after using the DWT as digital filter, results demonstrated that the proposed system is capable to reject high frequency noise and to detect drought conditions.

  9. Auxinic herbicides, mechanisms of action, and weed resistance: A look into recent plant science advances

    Directory of Open Access Journals (Sweden)

    Pedro Jacob Christoffoleti

    2015-08-01

    Full Text Available Auxin governs dynamic cellular processes involved at several stages of plant growth and development. In this review, we discuss the mechanisms employed by auxin in light of recent scientific advances, with a focus on synthetic auxins as herbicides and synthetic auxin resistance mechanisms. Two auxin receptors were reported. The plasma membrane receptor ABP1 (Auxin Binding Protein 1 alters the structure and arrangement of actin filaments and microtubules, leading to plant epinasty and reducing peroxisomes and mitochondria mobility in the cell environment. The second auxin receptor is the gene transcription pathway regulated by the SCFTir/AFB ubiquitination complex, which destroys transcription repressor proteins that interrupt Auxin Response Factor (ARF activation. As a result mRNA related with Abscisic Acid (ABA and ethylene are transcribed, producing high quantities of theses hormones. Their associated action leads to high production of Reactive Oxygen Species (ROS, leading to tissue and plant death. Recently, another ubiquitination pathway which is described as a new auxin signaling route is the F-box protein S-Phase Kinase-Associated Protein 2A (SKP2A. It is active in cell division regulation and there is evidence that auxin herbicides can deregulate the SKP2A pathway, which leads to severe defects in plant development. In this discussion, we propose that SFCSKP2A auxin binding site alteration could be a new auxinic herbicide resistance mechanism, a concept which may contribute to the current progress in plant biology in its quest to clarify the many questions that still surround auxin herbicide mechanisms of action and the mechanisms of weed resistance.

  10. Study of Pu consumption in advanced light water reactors: Evaluation of GE advanced boiling water reactor plants - compilation of Phase 1B task reports

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1993-09-15

    This report contains an extensive evaluation of GE advanced boiling water reactor plants prepared for United State Department of Energy. The general areas covered in this report are: core and system performance; fuel cycle; infrastructure and deployment; and safety and environmental approval.

  11. New steels for advanced coal fired plant up to 620{degree}C

    Energy Technology Data Exchange (ETDEWEB)

    Metcalfe, E.; Bakker, W.T.; Blum, R.; Bygate, R.P.; Gibbons, T.B.; Hald, J.; Matsuyama, F.; Naoi, H.; Price, S.; Sawaragi, Y. [National Power, Leatherhead (United Kingdom)

    1997-09-01

    There are strong environmental and economic incentives to increase the thermal efficiency of fossil fired power stations, and this has led to a steady increase in steam temperatures and pressures. In addition, the economics of power generation are driving down the price of plant so that advanced technology must be available at reduced installed cost. The key to securing these conditions is the development of high temperature materials, available at an acceptable price, particularly for thick section components in the boiler and turbine. These considerations led to the establishment of a four year EPRI project with partners from Japan, UK, USA and Denmark whose objective was to establish strong 9Cr and 12Cr steels as practical, validated materials for thick section boiler components such as headers and main steam lines. This successful project has developed three strong steels for thick section components for plant operating in the temperature range 565-620{degree}C and two of them, P92 (NF616) and P122(HCM12A), have received ASME Code approval. This project has now entered its second phase with the fabrication of full sized headers which have been installed in the NJV power plant in Denmark. In addition, there is further work on the long term microstructural stability of the steels, and a full sized pressure vessel test under accelerated conditions is about to start. 6 refs., 7 figs., 2 tabs.

  12. Constructs of analysis and assessment model of transformation restructuring in steel plants

    Directory of Open Access Journals (Sweden)

    B. Gajdzik

    2014-01-01

    Full Text Available The article presents the assumptions for construction of a transformation restructuring model which describes, in a complex way, the range of changes implemented in metallurgical enterprises in Poland in time of economical changes. The model was prepared on the basis of thorough research studies concerning restructuring in countries where transformation of the economy system took place, which is change from central planning economy into market economy. It was also based on scientific research and own experiences. Besides the presentation of the methodological assumptions of designing a model of transformation restructuring in metallurgical sector the article also shows the issue of transformation restructuring and the areas of implemented changes in particular ranges of activity in metallurgical enterprises, which allowed them to function in conditions of market economy.

  13. Genetic transformation of the plant pathogens Phytophthora capsici and Phytophthora parasitica

    National Research Council Canada - National Science Library

    Bailey, A M; Mena, G L; Herrera-Estrella, L

    1991-01-01

    ...) fused to promoter elements of the Ustilago maydis heat shock hsp70 gene. Enzymes Driselase and Novozyme 234 were used to generate protoplasts which were then transformed following exposure to plasmid DNA and polyethylene glycol 6000...

  14. SacB-SacR gene cassette as the negative selection marker to suppress Agrobacterium overgrowth in Agrobacterium-mediated plant transformation

    Directory of Open Access Journals (Sweden)

    Yiming Liu

    2016-10-01

    Full Text Available Agrobacterium overgrowth is a common problem in Agrobacterium-mediated plant transfor-mation. To suppress the Agrobacterium overgrowth, various antibiotics have been used during plant tissue culture steps. The antibiotics are expensive and may adversely affect plant cell differentiation and reduce plant transformation efficiency. The SacB-SacR proteins are toxic to most Agrobacterium tumefaciens strains when they are grown on culture medium sup¬plemented with sucrose. Therefore, SacB-SacR genes can be used as negative selection markers to suppress the overgrowth of Agrobacterium tumefaciens in the plant tissue culture process. We generated a mutant Agrobacterium tumefaciens strain GV2260 (recA-SacB/R that has the SacB-SacR cassette inserted into the bacterial genome at the recA gene locus. The mutant Agrobacterium strain is sensitive to sucrose but maintains its ability to transform plant cells in both transient and stable transformation assays. We demonstrated that the mutant strain GV2260 (recA-SacB/R can be inhibited by sucrose that reduces the overgrowth of Agrobacterium and therefore improves the plant transformation efficiency. We employed GV2260 (recA-SacB/R to generate stable transgenic N. benthamiana plants expressing a CRISPR-Cas9 for knocking out a WRKY transcrip¬tion factor.

  15. Reproduction of Meloidogyne javanica on Plant Roots Genetically Transformed by Agrobacterium rhizogenes

    OpenAIRE

    Verdejo, S.; Jaffee, B. A.; Mankau, R.

    1988-01-01

    Reproduction of Meloidogyne javanica was compared on several Agrobacterium rhizogenes-transformed root cultures under monoxenic conditions. M. javanica reproduced on all transformed roots tested; however, more females and eggs were obtained on potato and South Australian Early Dwarf Red tomato than on bindweed, Tropic tomato, lima bean, or carrot. Roots that grew at moderate rates into the agar and produced many secondary roots supported the highest reproduction. Numbers of females produced i...

  16. Analysis of propagation of Bacopa monnieri (L.) from hairy roots, elicitation and Bacoside A contents of Ri transformed plants.

    Science.gov (United States)

    Largia, Muthiah Joe Virgin; Satish, Lakkakula; Johnsi, Rajaiah; Shilpha, Jayabalan; Ramesh, Manikandan

    2016-08-01

    Agrobacterium rhizogenes mediated transformation has been experimented in leaf explants of the memory herb Bacopa monnieri in order to assess the regeneration potential of hairy roots (HR) followed by the elicitation of transformed plants for increased Bacoside A production. Out of the four strains tested, A4 and MTCC 532 derived HR exhibited regrowth in MS basal medium while MTCC 2364 derived HR showed regeneration in MS medium supplemented with suitable phyto hormones. R1000 derived HR possessed no regeneration potential. Comparable to A4, MTCC 532 derived HR displayed maximum regrowth frequency of about 85.71 ± 1.84 % with an increase in biomass to threefold. Therefore, five HR plant lines (MTCC 532 derived) were generated and maintained in MS basal liquid medium in which HR3 topped the others in producing a huge biomass of about 67.09 ± 0.66 g FW. PCR amplification and southern hybridization analysis of rol A gene (280 bp) has been performed in order to confirm the transformation process. Moreover, HR3 plant line has accumulated highest total phenolic content of about 165.68 ± 0.82 mg GAE/g DW and highest total flavonoid content of about 497.78 ± 0.57 mg QRE/g DW when compared to other lines and untransformed controls. In addition, HR3 plant extract showed 85.58 ± 0.14 % of DPPH (2, 2-diphenyl-1-picryl hydrazyl) inhibition displaying its reliable anti oxidant potential. Further on elicitation with 10 mg/L chitosan for 2 weeks, HR3 has produced 5.83 % of Bacoside A which is fivefold and threefold increased production when compared to untransformed and transformed unelicited controls respectively. This is the first report on eliciting HR plants for increased metabolite accumulation in B. monnieri.

  17. Progress of nuclear safety for symbiosis and sustainability advanced digital instrumentation, control and information systems for nuclear power plants

    CERN Document Server

    Yoshikawa, Hidekazu

    2014-01-01

    This book introduces advanced methods of computational and information systems allowing readers to better understand the state-of-the-art design and implementation technology needed to maintain and enhance the safe operation of nuclear power plants. The subjects dealt with in the book are (i) Full digital instrumentation and control systems and human?machine interface technologies (ii) Risk? monitoring methods for large and? complex? plants (iii) Condition monitors for plant components (iv) Virtual and augmented reality for nuclear power plants and (v) Software reliability verification and val

  18. Advancements in mass spectrometry for biological samples: Protein chemical cross-linking and metabolite analysis of plant tissues

    Energy Technology Data Exchange (ETDEWEB)

    Klein, Adam [Iowa State Univ., Ames, IA (United States)

    2015-01-01

    This thesis presents work on advancements and applications of methodology for the analysis of biological samples using mass spectrometry. Included in this work are improvements to chemical cross-linking mass spectrometry (CXMS) for the study of protein structures and mass spectrometry imaging and quantitative analysis to study plant metabolites. Applications include using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to further explore metabolic heterogeneity in plant tissues and chemical interactions at the interface between plants and pests. Additional work was focused on developing liquid chromatography-mass spectrometry (LC-MS) methods to investigate metabolites associated with plant-pest interactions.

  19. Modelling Digital Knowledge Transfer: Nurse Supervisors Transforming Learning at Point of Care to Advance Nursing Practice

    Directory of Open Access Journals (Sweden)

    Carey Mather

    2017-05-01

    Full Text Available Limited adoption of mobile technology for informal learning and continuing professional development within Australian healthcare environments has been explained primarily as an issue of insufficient digital and ehealth literacy of healthcare professionals. This study explores nurse supervisors’ use of mobile technology for informal learning and continuing professional development both for their own professional practice, and in their role in modelling digital knowledge transfer, by facilitating the learning and teaching of nursing students in the workplace. A convenience sample of 27 nurse supervisors involved with guiding and supporting undergraduate nurses participated in one of six focus groups held in two states of Australia. Expanding knowledge emerged as the key theme of importance to this group of clinicians. Although nurse supervisors regularly browsed Internet sources for learning and teaching purposes, a mixed understanding of the mobile learning activities that could be included as informal learning or part of formal continuing professional development was detected. Participants need educational preparation and access to mobile learning opportunities to improve and maintain their digital and ehealth literacy to appropriately model digital professionalism with students. Implementation of mobile learning at point of care to enable digital knowledge transfer, augment informal learning for students and patients, and support continuing professional development opportunities is necessary. Embedding digital and ehealth literacy within nursing curricula will promote mobile learning as a legitimate nursing function and advance nursing practice.

  20. Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transformed Mid-Infrared Spectroscopy (FT-IR).

    Science.gov (United States)

    Badhan, Ajay; Wang, Yuxi; McAllister, Tim A

    2017-01-01

    Fourier transformed mid-infrared spectroscopy (FTIR) is a powerful tool for compositional analysis of plant cell walls (Acebes et al., Front Plant Sci 5:303, 2014; Badhan et al., Biotechnol Biofuels 7:1-15, 2014; Badhan et al., BioMed Res Int 2015: 562952, 2015; Roach et al., Plant Physiol 156:1351-1363, 2011). The infrared spectrum generates a fingerprint of a sample with absorption peaks corresponding to the frequency of vibrations between the bonds of the atoms making up the material. Here, we describe a method focused on the use of FTIR in combination with principal component analysis (PCA) to characterize the composition of the plant cell wall. This method has been successfully used to study complex enzyme saccharification processes like rumen digestion to identify recalcitrant moieties in low-quality forage which resist rumen digestion (Badhan et al., BioMed Res Int 2015: 562952, 2015), as well as to characterize cell wall mutant lines or transgenic lines expressing exogenous hydrolases (Badhan et al., Biotechnol Biofuels 7:1-15, 2014; Roach et al., Plant Physiol 156:1351-1363, 2011). The FTIR method described here facilitates high-throughput identification of the major compositional differences across a large set of samples in a low cost and nondestructive manner.

  1. An Agrobacterium tumefaciens Strain with Gamma-Aminobutyric Acid Transaminase Activity Shows an Enhanced Genetic Transformation Ability in Plants

    Science.gov (United States)

    Nonaka, Satoko; Someya, Tatsuhiko; Zhou, Sha; Takayama, Mariko; Nakamura, Kouji; Ezura, Hiroshi

    2017-01-01

    Agrobacterium tumefaciens has the unique ability to mediate inter-kingdom DNA transfer, and for this reason, it has been utilized for plant genetic engineering. To increase the transformation frequency in plant genetic engineering, we focused on gamma-aminobutyric acid (GABA), which is a negative factor in the Agrobacterium-plant interaction. Recent studies have shown contradictory results regarding the effects of GABA on vir gene expression, leading to the speculation that GABA inhibits T-DNA transfer. In this study, we examined the effect of GABA on T-DNA transfer using a tomato line with a low GABA content. Compared with the control, the T-DNA transfer frequency was increased in the low-GABA tomato line, indicating that GABA inhibits T-DNA transfer. Therefore, we bred a new A. tumefaciens strain with GABA transaminase activity and the ability to degrade GABA. The A. tumefaciens strain exhibited increased T-DNA transfer in two tomato cultivars and Erianthus arundinacues and an increased frequency of stable transformation in tomato. PMID:28220841

  2. Plant tissue culture independent Agrobacterium tumefaciens mediated In-planta transformation strategy for upland cotton (Gossypium hirsutum

    Directory of Open Access Journals (Sweden)

    Bipinchandra B. Kalbande

    2016-06-01

    Full Text Available A new method of transgenic development called “In-planta” transformation method, where Agrobacterium is used to infect the plantlets but the steps of in vitro regeneration of plants is totally avoided. In this study, we have reported a simple In-planta method for efficient transformation of diploid cotton Gossypium hirsutum cv LRK-516 Anjali using Agrobacterium tumefaciens EHA-105 harbouring recombinant binary vector plasmid pBinAR with Arabidopsis At-NPR1 gene. Four day old plantlets were used for transformation. A vertical cut was made at the junction of cotyledonary leaves, moderately bisecting the shoot tip and exposing meristem cells at apical meristem. This site was infected with Agrobacterium inoculum. The transgenic events obtained were tested positive for the presence of At-NPR1 gene with promoter nptII gene. They are also tested negative for vector backbone integration and Agrobacterium contamination in T0 events. With this method a transformation frequency of 6.89% was reported for the cv LRK-516.

  3. The production of class III plant peroxidases in transgenic callus cultures transformed with the rolB gene of Agrobacterium rhizogenes.

    Science.gov (United States)

    Shkryl, Y N; Veremeichik, G N; Bulgakov, V P; Avramenko, T V; Günter, E A; Ovodov, Y S; Muzarok, T I; Zhuravlev, Y N

    2013-10-10

    The production of plant peroxidases by plant cell cultures is of great interest because of the potential for industrial applications. We used plant cell cultures overexpressing the rolB gene to produce increased amounts of plant class III peroxidases. The rolB gene ensured the stable and permanent activation of peroxidase activity in the transformed callus cultures of different plants. In particular, the total peroxidase activity in transformed Rubia cordifolia cells was increased 23-86-fold, and the abundance of the major peroxidase gene transcripts was increased 17-125-fold (depending on the level of rolB expression) compared with non-transformed control calli. The peroxidase-activating effect of rolB was greater than that of other peroxidase inducers, such as external stresses and methyl jasmonate. Copyright © 2013 Elsevier B.V. All rights reserved.

  4. Recent advances in plant-mediated engineered gold nanoparticles and their application in biological system.

    Science.gov (United States)

    Siddiqi, Khwaja Salahuddin; Husen, Azamal

    2017-03-01

    For biosynthesis of gold nanoparticles different parts of a plant are used as they contain metabolites such as alkaloids, flavonoids, phenols, terpenoids, alcohols, sugars and proteins which act as reducing agents to produce nanoparticles. They also act as capping agent and stabilizer for them. They are used in medicine, agriculture and many other technologies. The attention is therefore focussed on all plant species which have either aroma or colour in their leaves, flowers or roots for the synthesis of nanoparticles because they all contain such chemicals which reduce the metal ions to metal nanoparticles. The size and morphology of gold nanoparticles is dependent on the biogenic-synthetic route, incubation time, temperature, concentration and pH of the solution. In this review, we have discussed the latest developments for the fabrication of gold nanoparticles from herbal extract, their characterization by UV-vis., Fourier transform infrared spectroscopy, transmission electron microscopy, scanning electron microscopy, X-ray diffraction, atomic force microscopy, energy-dispersive X-ray spectroscopy, dynamic light scattering and Zeta Potential techniques. Their application in drug delivery, cancer treatment, catalysis and as antimicrobial agent has also been discussed. Copyright © 2016 Elsevier GmbH. All rights reserved.

  5. Hyperspectral remote sensing for advanced detection of early blight (Alternaria solani) disease in potato (Solanum tuberosum) plants

    Science.gov (United States)

    Atherton, Daniel

    Early detection of disease and insect infestation within crops and precise application of pesticides can help reduce potential production losses, reduce environmental risk, and reduce the cost of farming. The goal of this study was the advanced detection of early blight (Alternaria solani) in potato (Solanum tuberosum) plants using hyperspectral remote sensing data captured with a handheld spectroradiometer. Hyperspectral reflectance spectra were captured 10 times over five weeks from plants grown to the vegetative and tuber bulking growth stages. The spectra were analyzed using principal component analysis (PCA), spectral change (ratio) analysis, partial least squares (PLS), cluster analysis, and vegetative indices. PCA successfully distinguished more heavily diseased plants from healthy and minimally diseased plants using two principal components. Spectral change (ratio) analysis provided wavelengths (490-510, 640, 665-670, 690, 740-750, and 935 nm) most sensitive to early blight infection followed by ANOVA results indicating a highly significant difference (p < 0.0001) between disease rating group means. In the majority of the experiments, comparisons of diseased plants with healthy plants using Fisher's LSD revealed more heavily diseased plants were significantly different from healthy plants. PLS analysis demonstrated the feasibility of detecting early blight infected plants, finding four optimal factors for raw spectra with the predictor variation explained ranging from 93.4% to 94.6% and the response variation explained ranging from 42.7% to 64.7%. Cluster analysis successfully distinguished healthy plants from all diseased plants except for the most mildly diseased plants, showing clustering analysis was an effective method for detection of early blight. Analysis of the reflectance spectra using the simple ratio (SR) and the normalized difference vegetative index (NDVI) was effective at differentiating all diseased plants from healthy plants, except for the

  6. Intelligent software system for the advanced control room of a nuclear power plant

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Soon Heung; Choi, Seong Soo; Park, Jin Kyun; Heo, Gyung Young [Korea Advanced Institute of Science and Technology, Taejon (Korea, Republic of); Kim, Han Gon [Korea Electric Power Research Institute, Taejon (Korea, Republic of)

    1997-12-31

    The intelligent software system for nuclear power plants (NPPs) has been conceptually designed in this study. Its design goals are to operate NPPs in an improved manner and to support operators` cognitive takes. It consists of six major modules such as {sup I}nformation Processing,{sup {sup A}}larm Processing,{sup {sup P}}rocedure Tracking,{sup {sup P}}erformance Diagnosis,{sup a}nd {sup E}vent Diagnosis{sup m}odules for operators and {sup M}alfunction Diagnosis{sup m}odule for maintenance personnel. Most of the modules have been developed for several years and the others are under development. After the completion of development, they will be combined into one system that would be main parts of advanced control rooms in NPPs. 5 refs., 4 figs. (Author)

  7. Advancing the Deployment of Utility-Scale Photovoltaic Plants in the Northeast

    Energy Technology Data Exchange (ETDEWEB)

    Lofaro R.; Villaran, M; Colli, A.

    2012-06-03

    As one of the premier research laboratories operated by the Department of Energy, Brookhaven National Laboratory (BNL) is pursuing an energy research agenda that focuses on renewable energy systems and will help to secure the nation's energy security. A key element of the BNL research is the advancement of grid-connected utility-scale solar photovoltaic (PV) plants, particularly in the northeastern part of the country where BNL is located. While a great deal of information has been generated regarding solar PV systems located in mostly sunny, hot, arid climates of the southwest US, very little data is available to characterize the performance of these systems in the cool, humid, frequently overcast climates experienced in the northeastern portion of the country. Recognizing that there is both a need and a market for solar PV generation in the northeast, BNL is pursuing research that will advance the deployment of this important renewable energy resource. BNL's research will leverage access to unique time-resolved data sets from the 37MWp solar array recently developed on its campus. In addition, BNL is developing a separate 1MWp solar research array on its campus that will allow field testing of new PV system technologies, including solar modules and balance of plant equipment, such as inverters, energy storage devices, and control platforms. These research capabilities will form the cornerstone of the new Northeast Solar Energy Research Center (NSERC) being developed at BNL. In this paper, an overview of BNL's energy research agenda is given, along with a description of the 37MWp solar array and the NSERC.

  8. Investigation on the design of human-system interface for advanced nuclear plant control room

    Energy Technology Data Exchange (ETDEWEB)

    Chuang, C. F.; Chou, H. P. [Dept. of Engineering and System Science, National Tsing Hua Univ., Hsinchu Taiwan (China)

    2006-07-01

    The Lungmen Nuclear Power Project (LMNPP), under construction in Taiwan, consists of two GE Advanced Boiling Water Reactor (ABWR) units, each with 1350 MW electrical output. Major Human-System Interfaces (HSIs) of LMNPP are different from traditional ones. Video display units (VDUs) are the main human-system interface for operators to manipulate and to know the status of the equipment and plant information. Based upon NUREG-0711, the applicable human factors engineering (HFE) guideline in the design of HSIs has been adopted. An important aspect of the Lungmen HFE program has been the direct involvement of the end user, Taiwan Power Company (TPC), throughout the design development and implementation to ensure not only that the process for the design is compliant with the HFE principles, but also that the necessary displays, control, and alarms are provided to support the identified personnel tasks. This paper reviews the applicable HFE principles and verification and validation (V and V) processes in the design of HSIs for the advanced LMNPP. This paper also presents three investigated topics of the LMNPP HSI design development and implementation process. From the perspective of licensing concern and experience feedback, the focus of this paper is on the topics of validation with simulator, alarm auto reset, and VDU operational configuration strategy. The objectives of investigating the latter topic were to ensure the VDU operational configuration strategy, after appropriate V and V, achieves its goals of reinforcing operation discipline and distributing operator crew task assignments and workload during typical operations, and to confirm the need for an intensive training program that addresses the knowledge and skill requirements of the operators to meet the task characteristics and the responses of the plant processes. The results to date and implications for going forward from this process are also presented. (authors)

  9. Transformation of the limonene synthase gene into peppermint (Mentha piperita L.) and preliminary studies on the essential oil profiles of single transgenic plants.

    Science.gov (United States)

    Krasnyanski, S; May, R A; Loskutov, A; Ball, T M; Sink, K C

    1999-08-01

    Agrobacterium-mediated and direct gene transfer into protoplasts using PEG were both successfully used to produce stable, transformed peppermint plants (Mentha×piperita L. cultivar Black Mitcham) with the limonene synthase gene. Stem internode explants found to possess a high level of organogenesis through adventitious shoot formation were subjected to Agrobacterium tumefaciens disarmed strain GV3101 (pMP90). Following the development of an efficient protoplast-to-plant cycle from stem-isolated protoplasts, they were used in direct gene transformations. In both cases the binary vector pGA643 carrying the nptII/GUS genes, both driven by the CaMV35S promoter, was used in preliminary plant-transformation studies. Later, GUS was replaced with the limonene synthase gene. Kanamycin was used as a selective agent in all transformation experiments to obtain both transformed protoplast-derived calli as well as putative transgenic shoots regenerated from internode explants. Both types of transformation resulted in transgenic plants which were detected using PCR and confirmed by Southern-blot hybridizations. Southern analysis revealed that the method of Agrobacterium-mediated transformation is superior to the direct DNA uptake into protoplasts with regard to the stability of the insert during the transformation event. Single transgenic plants were grown to 10% flowering in a greenhouse and the plants derived both by the Agrobacterium and the protoplast-derived methods were generally observed to have essential oil profiles characterized by a high-menthone, low-menthol, high-menthofuran and -pulegone content in comparison to a typical mid-west peppermint. Limonene varied only slightly, around 1.2%, in transgenic plants produced by both methods.

  10. Assessment of plausible bioindicators for plant performance in advanced wastewater treatment systems.

    Science.gov (United States)

    Pérez-Uz, Blanca; Arregui, Lucía; Calvo, Pilar; Salvadó, Humbert; Fernández, Natividad; Rodríguez, Eva; Zornoza, Andrés; Serrano, Susana

    2010-09-01

    Three full-scale advanced biological systems for nitrogen removal showing different efficiencies were assessed during one year, to investigate the protist communities supported in these wastewater treatment plants (WWTP). The main goal of this research was to explore the differences of these communities from those observed in conventional activated sludge systems. The final objective was to provide background support for the proposal of bioindicators in this type of biological systems, where scarce information was available until now, since only conventional systems had been previously studied from this point of view. Results obtained indicate that, in fact, protist population density and diversity in advanced systems for N-elimination are quite different from other wastewater systems studied before. A statistical approach through multivariate analysis was developed to search for association between protist species and physical-chemical system performance, and specifically N-removal efficiencies. The original hypothesis proposing that previous indicators from conventional systems are not adequate in advanced N-removal mechanisms was proved to be correct. Efficient processes on N-removal, despite what it had been usually found in conventional systems, show important flagellate and amoeba populations and these populations tend to reduce their abundances as nitrogen removal performance decreases (moderate to low). Ciliates are however less abundant in these N-removal efficient systems. Certain groups and genera of protist such as flagellates and small amoebae are thus proposed as indicative of high performance N-removal, while in this case the appearance of certain ciliates were indicative of low performance on N- or high organic matter removal (as COD) efficiencies. Copyright © 2010 Elsevier Ltd. All rights reserved.

  11. Monitoring systems online of oil for transformers of nuclear power plants; Sistemas de monitorizacion online del aceite para transformadores de potencia de Centrales

    Energy Technology Data Exchange (ETDEWEB)

    Sarandeses, S.

    2014-07-01

    The nuclear power plants are showing their concern due to the existence of recent failures related to the bulky transformers of power. These transformers are not security, but are important for the production of power as its failure can cause transient on the floor, reactor scram or shooting, that can cause interruptions in the production of energy or might force us to reduce the power of production The analysis of gases dissolved in transformer oil is recognized as a trial key to identify a submerged transformer failure in oil. With this analysis it is not possible to ensure that there is no damage in the transformer, but the probability of risk of this type of failure can be reduced. The industry recommended to equip the new large power transformers with oil online monitoring systems and in some cases also be It recommended its use in existing transformers. (Author)

  12. Metabolite profiling of Arabidopsis thaliana (L.) plants transformed with an antisense chalcone synthase gene

    DEFF Research Database (Denmark)

    Le Gall, G.; Metzdorff, Stine Broeng; Pedersen, Jan W.;

    2005-01-01

    A metabolite profiling study has been carried out on Arabidopsis thaliana (L.) Heynh. ecotype Wassilewskija and a series of transgenic lines of the ecotype transformed with a CHS (chalcone synthase) antisense construct. Compound identifications by LC/MS and H-1 NMR are discussed. The glucosinolate...

  13. Efficient production of transgenic plants by Agrobacterium-mediated transformation of cassava (Manihot esculenta Crantz)

    NARCIS (Netherlands)

    Schreuder, M.M.; Raemakers, C.J.J.M.; Jacobsen, E.; Visser, R.G.F.

    2001-01-01

    An efficient and reproducible method was developed for Agrobacterium-mediated transformation of embryogenic suspension cultures of cassava. LBA4404(pTOK233), containing the nptII, hph and gus marker genes, was used in the experiments. Chemical selection by means of kanamycin was used to establish 10

  14. Improved phytoaccumulation of cadmium by genetically modified tobacco plants (Nicotiana tabacum L.). Physiological and biochemical response of the transformants to cadmium toxicity

    Energy Technology Data Exchange (ETDEWEB)

    Gorinova, N. [AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164 Sofia (Bulgaria)]. E-mail: noraig60@yahoo.co.uk; Nedkovska, M. [AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164 Sofia (Bulgaria); Todorovska, E. [AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164 Sofia (Bulgaria); Simova-Stoilova, L. [Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113 Sofia (Bulgaria); Stoyanova, Z. [Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113 Sofia (Bulgaria); Georgieva, K. [Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113 Sofia (Bulgaria); Demirevska-Kepova, K. [Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113 Sofia (Bulgaria); Atanassov, A. [AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164 Sofia (Bulgaria); Herzig, R. [Phytotech-Foundation PT-F, Quartiergasse 12, CH 3013 Bern (Switzerland)

    2007-01-15

    The response of tobacco plants (Nicotiana tabacum L.)-non-transformed and transformed with a metallothionein gene MThis from Silene vulgaris L. - to increase cadmium supply in the nutrient solution was compared. The transgenic plants accumulated significantly more Cd both in the roots and the leaves. Visual toxicity symptoms and disturbance in water balance were correlated with Cd tissue content. Treatment with 300 {mu}M CdCl{sub 2} resulted in inhibition of photosynthesis and mobilization of the ascorbate-glutathione cycle. Treatment with 500 {mu}M CdCl{sub 2} led to irreversible damage of photosynthesis and oxidative stress. An appearance of a new peroxidase isoform and changes in the leaf polypeptide pattern were observed at the highest Cd concentration. The level of non-protein thiols gradually increased following the Cd treatment both in transgenic and non-transformed plants. - Genetic transformation of Nicotiana tabacum L. by metallothionein gene improved phytoaccumulation of cadmium.

  15. Conservation state of populations of rare plant species in highly transformed meadow steppes of Southern Opillya

    Directory of Open Access Journals (Sweden)

    I. I. Dmytrash-Vatseba

    2016-09-01

    Full Text Available Degradation of natural habitats causes rapid extinction of rare plant populations. The diversity of rare plant species in the meadow steppes of Southern Opillya (Western Ukraine depends strongly on patch area, pasture digression of vegetation and a variety of eco-coenotical conditions. The main threats for the rare components of the meadow steppe flora are reduction of habitat and overgrazing. Spatial connections between sites are unable to support a constant rare plant population. The analysis of the composition of rare plant meadow-steppe species indicated that habitats with similar rare species composition usually have similar parameters of area, stages of pasture digression and eco-coenotical conditions. Spatial connectivity of patches does not ensure species similarity of rare components of the flora. Rare plant species were grouped according to their preferences for habitat , area and condition. In small patches subject to any stage of pasture digression grow populations of Adonis vernalis L., Pulsatilla patens (L. Mill., P. grandis Wender., Stipa capillata L., S. рennata L., Chamaecytisus blockianus (Pawł. Klásková etc. On the contrary, populations of other species (Carlina onopordifolia Besser. ex Szafer., Kuecz. et Pawł., Adenophora liliifolia (L. Ledeb. ex A. DC., Crambe tataria Sebeók, Euphorbia volhynica Besser ex Racib., Stipa tirsa Stev. etc. prefer large habitats, not changed by pasture digression. Prevention of reduction of rare species diversity requires preservation (also extension of patch area and regulation of grazing intensity.

  16. Transformation of traditional knowledge of medicinal plants: the case of Tyroleans (Austria who migrated to Australia, Brazil and Peru

    Directory of Open Access Journals (Sweden)

    Pirker Heidemarie

    2012-11-01

    Full Text Available Abstract Background In ethnobotanical research, the investigation into traditional knowledge of medicinal plants in the context of migration has been of increasing interest in recent decades since it is influenced and changed by new environmental and social conditions. It most likely undergoes transformation processes to match the different living circumstances in the new location. This study compares the traditional knowledge of medicinal plants held by Tyroleans – and their descendants – who emigrated to Australia, Brazil and Peru at different time scales. The study’s findings allow a discussion of the complexities and dynamics that influence this knowledge within the context of long-distance migration. Methods Information was obtained from 65 informants by free-listing, semi-structured interviews and non-participatory observation in Tyrol (Austria and the migrants’ countries: Australia, Brazil and Peru. The collected data was analysed using different quantitative approaches, including statistical tests, and compared between the countries of investigation. Results All respondents in all four investigation areas claimed that they had knowledge and made use of medicinal plants to treat basic ailments in their day-to-day lives. Informants made 1,139 citations of medicinal plants in total in free lists, which correspond to 164 botanical taxa (genus or species level in Tyrol, 87 in Australia, 84 in Brazil and 134 in Peru. Of all the botanical taxa listed, only five (1.1% were listed in all four countries under investigation. Agreement among informants within free lists was highest in Tyrol (17%, followed by Peru (12.2%, Australia (11.9% and Brazil (11.2%. The proportion of agreement differs significantly between informants in Australia and Tyrol (p = 0.001, Brazil and Tyrol (p = 0.001 and Peru and Tyrol (p = 0.001 and is similar between informants in the migrant countries, as indicated by statistical tests. We recorded 1,286 use

  17. Physical Metallurgy of Modern Creep-Resistant Steel for Steam Power Plants: Microstructure and Phase Transformations

    Directory of Open Access Journals (Sweden)

    V. C. Igwemezie

    2016-01-01

    Full Text Available The fact that the microstructure of steel depends on its composition and the heat treatment given to it has been heavily exploited in the design of steel for power plant applications. To obtain a steel that can function at the higher temperature where power plants operate without failure for extended life, heat treatment is needed to produce fine and highly stable dispersion of carbides, nitrides, and intermetallic compounds in the microstructure of the material. A significant contribution also comes from solid solution strengthening by substitutional solutes. We review here various types of phases, microstructures, functions, and interacting effects of the various alloying elements in the design of steel for modern power plant application.

  18. Role of plants in nitrogen and sulfur transformations in floating hydroponic root mats: A comparison of two helophytes.

    Science.gov (United States)

    Saad, Rania A B; Kuschk, Peter; Wiessner, Arndt; Kappelmeyer, Uwe; Müller, Jochen A; Köser, Heinz

    2016-10-01

    Knowledge about the roles helophytes play in constructed wetlands (CWs) is limited, especially regarding their provision of organic rhizodeposits. Here, transformations of inorganic nitrogen and sulfur were monitored in a CW variety, floating hydroponic root mat (FHRM), treating synthetic wastewater containing low concentration of organic carbon. Two helophytes, Phragmites australis and Juncus effusus, were compared in duplicates. Striking differences were found between the FHRM of the two helophytes. Whereas ammonium was removed in all FHRMs to below detection level, total nitrogen of 1.15 ± 0.4 g m(-2) d(-1) was removed completely only in P. australis systems. The mats with J. effusus displayed effective nitrification but incomplete denitrification as 77% of the removed ammonium-nitrogen accumulated as nitrate. Furthermore, the P. australis treatment units showed on average 3 times higher sulfate-S removal rates (1.1 ± 0.45 g m(-2) d(-1)) than the systems planted with J. effusus (0.37 ± 0.29 g m(-2) d(-1)). Since the influent organic carbon was below the stoichiometric requirement for the observed N and S transformation processes, helophytes' organic rhizodeposits apparently contributed to these transformations, while P. australis provided about 6 times higher bioavailable organic rhizodeposits than J. effusus. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. Ash chemistry in MSW incineration plants: Advanced characterization and thermodynamic considerations

    Energy Technology Data Exchange (ETDEWEB)

    Frandsen, Flemming J.; Laursen, Karin; Arvelakis, S. (and others)

    2004-07-15

    A number of ash samples where collected at four Danish municipal solid waste incineration (MSWI) plants. Samples of bottom ash/slag, 2nd-3rd pass ashes and ESP/E-filter ash were collected at the plants. The ashes were analyzed by a number of standard chemical analyses, and a number of advanced analytical techniques. The wet chemical analyses of the different ash fractions revealed that residual ash is formed on the grate by interaction of the main ash forming elements, Al, Ca, Fe and Si. Some of this ash is entrained from the grate and carried with the flue gas along the flue gas duct, where volatile species of K, Na, Pb, Zn, Cl and S starts to condense heterogeneously on the fly ash, thereby causing a dilution of the main ash forming elements. When compared plant-by-plant, the ash chemical analyses showed that the plant with the highest S-content in the fly ash is the one with the most often operational problems in relation to deposition, while a high Cl-content is indicative of a high corrosive potential. An existing Computer Controlled Scanning Electron Microscopy (CCSEM) algorithm was extended with chemical classes covering Pb- and Zn-rich phases. This has made it possible also to analyze MSW-derived ashes by use of CCSEM. Representative samples of 2nd-3rd pass and ESP/E-filter ashes from the four plants have been analyzed by Quantitative X-Ray Diffraction (QXRD) analysis. Only a few crystalline phases were identified: KCl, NaCl, CaSO{sub 4}, SiO{sub 2} and CaCO{sub 3} being the main ones. No crystalline phases containing Pb or Zn were identified by QXRD. A comparison between CCSEM and QXRD revealed the expected surface nature of the CCSEM analysis. Samples of 2nd-3rd pass and ESP/E-filter ash from the four plants where investigated for melting behavior in the Simultaneous Thermal Analyzer (STA). It was shown that it is possible to quantify the melting behavior of these ashes, and that the melting goes on in two steps (salts followed by silicates/oxides). The

  20. How tests of lubricating and transformer oils became part of power plant chemistry in Denmark

    Energy Technology Data Exchange (ETDEWEB)

    Moeller, H. [I/S Nordjyllandsvaerket, Vodskov (Denmark)

    1996-12-01

    Lubricating, hydraulic and transformer oils based on refined crude oil are used in nearly all power station components, such as gear, turbines, hydraulic stations, feed pumps and transformers. The function of these components totally depends on the condition of the oils and their properties. Seen from this point one may wonder why examination and evaluation of oils did not become part of the power station chemistry within the ELSAM utility area until during the middle of the eighties. We started to examine the properties of lubricating oils at the time when several steam turbines experienced serious problems with formation of deposits in their hydraulic control circuits. This work was intensified in connection with the significant number of CHPs and wind turbines erected within the Danish electricity sector during the past 10 years or so. The majority of the CHPs are natural gas fired turbines or motors, equipment which severely stresses the lubricating oil. In collaboration with KEMA, the Netherlands, we have carried through with a large examination of lubricating oils in gas turbines and we have found suitable oil types. The objectives of our work with lubricating and transformer oils have been to link together the laboratory measurements with operational experience. Only by doing this is it possible to utilize the laboratory measurements in a correct way. It must be remembered that the main part of all oil specifications concerns the properties of new oils. Only very little is published about the requirements concerning used oils. (EG)

  1. An improved plant regeneration and Agrobacterium - mediated transformation of red pepper (Capsicum annuum L.).

    Science.gov (United States)

    Kumar, R Vinoth; Sharma, V K; Chattopadhyay, B; Chakraborty, S

    2012-10-01

    Capsicum annuum (red pepper) is an important spice cum vegetable crop in tropical and subtropical countries. Here, we report an effective and reproducible auxin free regeneration method for six different red pepper cultivars (ACA-10, Kashi Anmol, LCA-235, PBC-535, Pusa Jwala and Supper) using hypocotyl explants and an efficient Agrobacterium-mediated transformation protocol. The explants (hypocotyls, cotyledonary leaves and leaf discs) collected from axenic seedlings of six red pepper cultivars were cultured on either hormone free MS medium or MS medium supplemented with BAP alone or in combination with IAA. Inclusion of IAA in the regeneration medium resulted in callus formation at the cut ends of explants, formation of rosette leaves and ill defined shoot buds. Regeneration of shoot buds could be achieved from hypocotyls grown in MS medium supplemented with different concentrations of BAP unlike other explants which failed to respond. Incorporation of GA3 in shoot elongation medium at 0.5 mg/l concentration enhanced the elongation in two cultivars, LCA-235 and Supper, while other cultivars showed no significant response. Chilli cultivar, Pusa Jwala was transformed with βC1 ORF of satellite DNA β molecule associated with Chilli leaf curl Joydebpur virus through Agrobacterium tumefaciens. Transgene integration in putative transformants was confirmed by PCR and Southern hybridization analysis.

  2. Assessing the fate and transformation of plant residues in the terrestrial environment using HR-MAS NMR spectroscopy

    Science.gov (United States)

    Kelleher, Brian P.; Simpson, Myrna J.; Simpson, Andre J.

    2006-08-01

    Plant litter decomposition plays a fundamental role in carbon and nitrogen cycles, provides key nutrients to the soil environment and represents a potentially large positive feedback to atmospheric CO 2. However, the full details of decomposition pathways and products are unknown. Here we present the first application of HR-MAS NMR spectroscopy on 13C and 15N labeled plant materials, and apply this approach in a preliminary study to monitor the environmental degradation of the pine and wheatgrass residues over time. In HR-MAS, is it possible to acquire very high resolution NMR data of plant biomass, and apply the vast array of multidimensional experiments available in conventional solution-state NMR. High levels of isotopic enrichment combined with HR-MAS significantly enhance the detection limits, and provide a wealth of information that is unattainable by any other method. Diffusion edited HR-MAS NMR data reveal the rapid loss of carbohydrate structures, while two-dimensional (2-D) HR-MAS NMR spectra demonstrate the relatively fast loss of both hydrolysable and condensed tannin structures from all plant tissues studied. Aromatic (partially lignin) and aliphatic components (waxes, cuticles) tend to persist, along with a small fraction of carbohydrate, and become highly functionalized over time. While one-dimensional (1-D) 13C HR-MAS NMR spectra of fresh plant tissue reflect compositional differences between pine and grass, these differences become negligible after decomposition suggesting that recalcitrant carbon may be similar despite the plant source. Two-dimensional 1H- 15N HR-MAS NMR analysis of the pine residue suggests that nitrogen from specific peptides is either selectively preserved or used for the synthesis of what appears to be novel structures. The amount of relevant data generated from plant components in situ using HR-MAS NMR is highly encouraging, and demonstrates that complete assignment will yield unprecedented structural knowledge of plant cell

  3. Recent advances in the research for the homolog of breast cancer associated gene AtROW1 in higher plants.

    Science.gov (United States)

    Jiao, Yue; Zhang, Yuzhou; Zhu, Yu-Xian

    2016-08-01

    BARD1 (BRCA1 associated RING domain protein 1), as an important animal tumor suppressor gene associated with many kinds of cancers, has been intensively studied for decades. Surprisingly, homolog of BARD1 was found in plants and it was renamed AtROW1 (repressor of Wuschel-1) according to its extremely important function with regard to plant stem cell homeostasis. Although great advances have been made in human BARD1, the function of this animal tumor-suppressor like gene in plant is not well studied and need to be further elucidated. Here, we review and summarize past and present work regarding this protein. Apart from its previously proposed role in DNA repair, recently it is found essential for shoot and root stem cell development and differentiation in plants. The study of AtROW1 in plant may provide an ideal model for further elucidating the functional mechanism of BARD1 in mammals.

  4. Gladiolus plants transformed with single-chain variable fragment antibodies to Cucumber mosaic virus

    Science.gov (United States)

    Transgenic plants of Gladiolus ‘Peter Pears’ or ‘Jenny Lee’ were developed that contain single-chain variable fragments (scFv) to Cucumber mosaic virus (CMV) subgroup I or II. The CMV subgroup I heavy and light chain scFv fragments were placed under control of either the duplicated CaMV 35S or suga...

  5. Mineralization and Transformation of Nitrogen Derived from Plant Materials in Soils over 10 Years

    Institute of Scientific and Technical Information of China (English)

    CHENGLI-LI; WENQI-XIAO

    1993-01-01

    Results of a 10-year decomposition experiment indicated that the annual mineralization rate of organic N in newly-formed humus varied with the type of original plant materials and the water regimes for decomposition,ranging from 0.028 to 0.074.The mineralization rate under waterlogged conditions was higher than that under upland conditions.The proportion of α-amino acid N in humus newly-formed under waterlogged conditions was slightly higher than that under upland conditions.It decreased gradually with time,while the proportion of nonhydrolyzable N showed no consistent trend,irrespective of the water regines for decomposition.The distribution of amino acids in humus newly-formed from different plant materials under various water regimes was quite similar with that in original plant materials,and only minor differences could be found among them.For example,in comparison to original plant materials,the newly-formed humus contained higher proportions of isoleucine,cysting,γ-amino-butyric acid and ornithine,and lower proportions of phenylalanine and proline.Moreover the proportion of phenylalanine was higher in the humus newly-fored under waterlogged conditions than that under upland conditions.

  6. Modeling Creep-Fatigue-Environment Interactions in Steam Turbine Rotor Materials for Advanced Ultra-supercritical Coal Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Shen, Chen [General Electric Global Research, Niskayuna, NY (United States)

    2014-04-01

    The goal of this project is to model creep-fatigue-environment interactions in steam turbine rotor materials for advanced ultra-supercritical (A-USC) coal power Alloy 282 plants, to develop and demonstrate computational algorithms for alloy property predictions, and to determine and model key mechanisms that contribute to the damages caused by creep-fatigue-environment interactions.

  7. Recent advances in creep-resistant steels for power plant applications

    Indian Academy of Sciences (India)

    P J Ennis; A Czyrska-Filemonowicz

    2003-06-01

    The higher steam temperatures and pressures required to achieve increase in thermal efficiency of fossil fuel-fired power-generation plants necessitate the use of steels with improved creep rupture strength. The 9% chromium steels developed during the last three decades are of great interest in such applications. In this report, the development of steels P91, P92 and E911 is described. It is shown that the martensitic transformation in these three steels produces high dislocation density that confers significant transient hardening. However, the dislocation density decreases during exposure at service temperatures due to recovery effects and for long-term creep strength the sub-grain structure produced under different conditions is most important. The changes in the microstructure mean that great care is needed in the extrapolation of experimental data to obtain design values. Only data from tests with rupture times above 3,000 h provide reasonable extrapolated values. It is further shown that for the 9% chromium steels, oxidation resistance in steam is not sufficiently high for their use as thin-walled components at temperatures of 600°C and above. The potential for the development of steels of higher chromium contents (above 11%) to give an improvement in steam oxidation resistance whilst maintaining creep resistance to the 9% chromium steels is discussed.

  8. Recent Advances in the Application of Metabolomics to Studies of Biogenic Volatile Organic Compounds (BVOC Produced by Plant

    Directory of Open Access Journals (Sweden)

    Yoko Iijima

    2014-08-01

    Full Text Available In many plants, biogenic volatile organic compounds (BVOCs are produced as specialized metabolites that contribute to the characteristics of each plant. The varieties and composition of BVOCs are chemically diverse by plant species and the circumstances in which the plants grow, and also influenced by herbivory damage and pathogen infection. Plant-produced BVOCs are receptive to many organisms, from microorganisms to human, as both airborne attractants and repellants. In addition, it is known that some BVOCs act as signals to prime a plant for the defense response in plant-to-plant communications. The compositional profiles of BVOCs can, thus, have profound influences in the physiological and ecological aspects of living organisms. Apart from that, some of them are commercially valuable as aroma/flavor compounds for human. Metabolomic technologies have recently revealed new insights in biological systems through metabolic dynamics. Here, the recent advances in metabolomics technologies focusing on plant-produced BVOC analyses are overviewed. Their application markedly improves our knowledge of the role of BVOCs in chemosystematics, ecological influences, and aroma research, as well as being useful to prove the biosynthetic mechanisms of BVOCs.

  9. A Visual Basic simulation software tool for performance analysis of a membrane-based advanced water treatment plant.

    Science.gov (United States)

    Pal, P; Kumar, R; Srivastava, N; Chowdhury, J

    2014-02-01

    A Visual Basic simulation software (WATTPPA) has been developed to analyse the performance of an advanced wastewater treatment plant. This user-friendly and menu-driven software is based on the dynamic mathematical model for an industrial wastewater treatment scheme that integrates chemical, biological and membrane-based unit operations. The software-predicted results corroborate very well with the experimental findings as indicated in the overall correlation coefficient of the order of 0.99. The software permits pre-analysis and manipulation of input data, helps in optimization and exhibits performance of an integrated plant visually on a graphical platform. It allows quick performance analysis of the whole system as well as the individual units. The software first of its kind in its domain and in the well-known Microsoft Excel environment is likely to be very useful in successful design, optimization and operation of an advanced hybrid treatment plant for hazardous wastewater.

  10. Development of an efficient Agrobacterium-mediated transformation system and production of herbicide-resistant transgenic plants in garlic (Allium sativum L.).

    Science.gov (United States)

    Ahn, Yul-Kyun; Yoon, Moo-Kyoung; Jeon, Jong-Seong

    2013-08-01

    The genetic improvement of garlic plants (Allium sativum L.) with agronomical beneficial traits is rarely achieved due to the lack of an applicable transformation system. Here, we developed an efficient Agrobacterium-mediated transformation procedure with Danyang, an elite Korean garlic cultivar. Examination of sGFP (synthetic green fluorescence protein) expression revealed that treatment with 2-(N-morpholino) ethanesulfonic acid (MES), L-cysteine and/or dithiothreitol (DTT) gives the highest efficiency in transient gene transfer during Agrobacterium co-cultivation with calli derived from the roots of in vitro plantlets. To increase stable transformation efficiency, a two-step selection was employed on the basis of hygromycin resistance and sGFP expression. Of the hygromycin-resistant calli initially produced, only sGFP-expressing calli were subcultured for selection of transgenic calli. Transgenic plantlets produced from these calli were grown to maturity. The transformation efficiency increased up to 10.6% via our optimized procedure. DNA and RNA gel-blot analysis indicated that transgenic garlic plants stably integrated and expressed the phosphinothricin acetyltransferase (PAT) gene. A herbicide spraying assay demonstrated that transgenic plants of garlic conferred herbicide resistance, whilst nontransgenic plants and weeds died. These results indicate that our transformation system can be efficiently utilized to produce transgenic garlic plants with agronomic benefits.

  11. Degradation of chlorophenols and alkylphenol ethoxylates, two representative textile chemicals, in water by advanced oxidation processes: the state of the art on transformation products and toxicity.

    Science.gov (United States)

    Karci, Akin

    2014-03-01

    Advanced oxidation processes based on the generation of reactive species including hydroxyl radicals are viable options in eliminating a wide array of refractory organic contaminants in industrial effluents. The assessment of transformation products and toxicity should be, however, the critical point that would allow the overall efficiency of advanced oxidation processes to be better understood and evaluated since some transformation products could have an inhibitory effect on certain organisms. This article reviews the most recent studies on transformation products and toxicity for evaluating advanced oxidation processes in eliminating classes of compounds described as "textile chemicals" from aqueous matrices and poses questions in need of further investigation. The scope of this paper is limited to the scientific studies with two classes of textile chemicals, namely chlorophenols and alkylphenol ethoxylates, whose use in textile industry is a matter of debate due to health risks to humans and harm to the environment. The article also raises the critical question: What is the state of the art knowledge on relationships between transformation products and toxicity?

  12. A benchmark simulation model to describe plant-wide phosphorus transformations in WWTPs

    DEFF Research Database (Denmark)

    Flores-Alsina, Xavier; Ikumi, D.; Kazadi-Mbamba, C.

    It is more than 10 years since the publication of the BSM1 technical report (Copp, 2002). The main objective of BSM1 was to create a platform for benchmarking C and N removal strategies in activated sludge systems. The initial platform evolved into BSM1_LT and BSM2, which allowed for the evaluati...... to be addressed and presents the simulation results of the first software prototype....... of monitoring and plant-wide control strategies, respectively. In addition, researchers working within the IWA Task Group on Benchmarking of Control Strategies for Wastewater Treatment Plants developed other BSM related spin-off products, such as the dynamic influent generator, sensor/actuators/fault models......) pursue biological/chemical phosphorus removal. However, realistic descriptions of combined C, N and P removal, adds a major, but unavoidable degree of complexity in wastewater treatment process models. This paper identifies and discusses important issues that need to be addressed to upgrade the BSM2...

  13. [Advanced Treatment of Effluent from Industrial Park Wastewater Treatment Plant by Ferrous Ion Activated Sodium Persulfate].

    Science.gov (United States)

    Zhu, Song-mei; Zhou, Zhen; Gu, Ling-yun; Jiang, Hai-tao; Ren, Jia-min; Wang, Luo-chun

    2016-01-15

    Fe(II) activated sodium persulfate (PS) technology was used for advanced treatment of effluent from industrial park wastewater treatment plant. Separate and combined effects of PS/COD, Fe(II)/PS and pH on COD and TOC removal were analyzed by the response surface methodology. Variations of organic substances before and after Fe(II)-PS oxidation were characterized by UV-Vis spectrometry, gel chromatography and three-dimensional fluorescence. PS/COD and Fe(II)/PS had significant effect on COD removal, while all the three factors had significant effect on TOC removal. The combined effect of PS/COD and pH had significant effect on COD removal. COD and TOC removal efficiencies reached 50.7% and 60.6% under optimized conditions of PS/COD 3.47, Fe(II)/PS 3.32 and pH 6.5. Fe(II)-PS oxidation converted macromolecular organic substances to small ones, and reduced contents of protein-, humic- and fulvic-like substances.

  14. Gas centrifuge enrichment plants inspection frequency and remote monitoring issues for advanced safeguards implementation

    Energy Technology Data Exchange (ETDEWEB)

    Boyer, Brian David [Los Alamos National Laboratory; Erpenbeck, Heather H [Los Alamos National Laboratory; Miller, Karen A [Los Alamos National Laboratory; Ianakiev, Kiril D [Los Alamos National Laboratory; Reimold, Benjamin A [Los Alamos National Laboratory; Ward, Steven L [Los Alamos National Laboratory; Howell, John [GLASGOW UNIV.

    2010-09-13

    Current safeguards approaches used by the IAEA at gas centrifuge enrichment plants (GCEPs) need enhancement in order to verify declared low enriched uranium (LEU) production, detect undeclared LEU production and detect high enriched uranium (BEU) production with adequate probability using non destructive assay (NDA) techniques. At present inspectors use attended systems, systems needing the presence of an inspector for operation, during inspections to verify the mass and {sup 235}U enrichment of declared cylinders of uranium hexafluoride that are used in the process of enrichment at GCEPs. This paper contains an analysis of how possible improvements in unattended and attended NDA systems including process monitoring and possible on-site destructive analysis (DA) of samples could reduce the uncertainty of the inspector's measurements providing more effective and efficient IAEA GCEPs safeguards. We have also studied a few advanced safeguards systems that could be assembled for unattended operation and the level of performance needed from these systems to provide more effective safeguards. The analysis also considers how short notice random inspections, unannounced inspections (UIs), and the concept of information-driven inspections can affect probability of detection of the diversion of nuclear material when coupled to new GCEPs safeguards regimes augmented with unattended systems. We also explore the effects of system failures and operator tampering on meeting safeguards goals for quantity and timeliness and the measures needed to recover from such failures and anomalies.

  15. Production of potato minitubers using advanced environmental control technologies developed for growing plants in space

    Science.gov (United States)

    Britt, Robert G.

    1998-01-01

    Development of plant growth systems for use in outer space have been modified for use on earth as the backbone of a new system for rapid growth of potato minitubers. The automation of this new biotechnology provides for a fully controllable method of producing pathogen-free nuclear stock potato minitubers from tissue cultured clones of varieties of potato in a biomanufacturing facility. These minitubers are the beginning stage of seed potato production. Because the new system provides for pathogen-free minitubers by the tens-of-millions, rather than by the thousands which are currently produced in advanced seed potato systems, a new-dimension in seed potato development, breeding and multiplication has been achieved. The net advantage to earth-borne agricultural farming systems will be the elimination of several years of seed multiplication from the current system, higher quality potato production, and access to new potato varieties resistant to diseases and insects which will eliminate the need for chemical controls.

  16. Hadamard Transforms

    CERN Document Server

    Agaian, Sos; Egiazarian, Karen; Astola, Jaakko

    2011-01-01

    The Hadamard matrix and Hadamard transform are fundamental problem-solving tools in a wide spectrum of scientific disciplines and technologies, such as communication systems, signal and image processing (signal representation, coding, filtering, recognition, and watermarking), digital logic (Boolean function analysis and synthesis), and fault-tolerant system design. Hadamard Transforms intends to bring together different topics concerning current developments in Hadamard matrices, transforms, and their applications. Each chapter begins with the basics of the theory, progresses to more advanced

  17. Multiple abiotic stress tolerance of the transformants yeast cells and the transgenic Arabidopsis plants expressing a novel durum wheat catalase.

    Science.gov (United States)

    Feki, Kaouthar; Kamoun, Yosra; Ben Mahmoud, Rihem; Farhat-Khemakhem, Ameny; Gargouri, Ali; Brini, Faiçal

    2015-12-01

    Catalases are reactive oxygen species scavenging enzymes involved in response to abiotic and biotic stresses. In this study, we described the isolation and functional characterization of a novel catalase from durum wheat, designed TdCAT1. Molecular Phylogeny analyses showed that wheat TdCAT1 exhibited high amino acids sequence identity to other plant catalases. Sequence homology analysis showed that TdCAT1 protein contained the putative calmodulin binding domain and a putative conserved internal peroxisomal targeting signal PTS1 motif around its C-terminus. Predicted three-dimensional structural model revealed the presence of four putative distinct structural regions which are the N-terminal arm, the β-barrel, the wrapping and the α-helical domains. TdCAT1 protein had the heme pocket that was composed by five essential residues. TdCAT1 gene expression analysis showed that this gene was induced by various abiotic stresses in durum wheat. The expression of TdCAT1 in yeast cells and Arabidopsis plants conferred tolerance to several abiotic stresses. Compared with the non-transformed plants, the transgenic lines maintained their growth and accumulated more proline under stress treatments. Furthermore, the amount of H2O2 was lower in transgenic lines, which was due to the high CAT and POD activities. Taken together, these data provide the evidence for the involvement of durum wheat catalase TdCAT1 in tolerance to multiple abiotic stresses in crop plants. Copyright © 2015 Elsevier Masson SAS. All rights reserved.

  18. Mirror Advanced Reactor Study (MARS). Final report. Volume 1-B. Commercial fusion electric plant

    Energy Technology Data Exchange (ETDEWEB)

    Donohue, M.L.; Price, M.E. (eds.)

    1984-07-01

    Volume 1-B contains the following chapters: (1) blanket and reflector; (2) central cell shield; (3) central cell structure; (4) heat transport and energy conversion; (5) tritium systems; (6) cryogenics; (7) maintenance; (8) safety; (9) radioactivity, activation, and waste disposal; (10) instrumentation and control; (11) balance of plant; (12) plant startup and operation; (13) plant availability; (14) plant construction; and (15) economic analysis.

  19. Fourier transform mid infrared spectroscopy applications for monitoring the structural plasticity of plant cell walls

    Science.gov (United States)

    Largo-Gosens, Asier; Hernández-Altamirano, Mabel; García-Calvo, Laura; Alonso-Simón, Ana; Álvarez, Jesús; Acebes, José L.

    2014-01-01

    Fourier transform mid-infrared (FT-MIR) spectroscopy has been extensively used as a potent, fast and non-destructive procedure for analyzing cell wall architectures, with the capacity to provide abundant information about their polymers, functional groups, and in muro entanglement. In conjunction with multivariate analyses, this method has proved to be a valuable tool for tracking alterations in cell walls. The present review examines recent progress in the use of FT-MIR spectroscopy to monitor cell wall changes occurring in muro as a result of various factors, such as growth and development processes, genetic modifications, exposition or habituation to cellulose biosynthesis inhibitors and responses to other abiotic or biotic stresses, as well as its biotechnological applications. PMID:25071791

  20. Fourier transform Raman spectroscopic characterisation of cells of the plant-associated soil bacterium Azospirillum brasilense Sp7

    Science.gov (United States)

    Kamnev, A. A.; Tarantilis, P. A.; Antonyuk, L. P.; Bespalova, L. A.; Polissiou, M. G.; Colina, M.; Gardiner, P. H. E.; Ignatov, V. V.

    2001-05-01

    Structural and compositional features of bacterial cell samples and of lipopolysaccharide-protein complex isolated from the cell surface of the plant-growth-promoting rhizobacterium Azospirillum brasilense (wild-type strain Sp7) were characterised using Fourier transform (FT) Raman spectroscopy. The structural spectroscopic information obtained is analysed and considered together with analytical data on the content of metal cations (Co 2+, Cu 2+ and Zn 2+) in the bacterial cells grown in a standard medium as well as in the presence of each of the cations (0.2 mM). The latter, being taken up by bacterial cells from the culture medium in significant amounts, were shown to induce certain metabolic changes in the bacterium revealed in FT-Raman spectra, which is discussed from the viewpoint of bacterial response to environmental stresses.

  1. Soil ni.trogen transformations varied with plant community under Nanchang urban forests in mid-subtropical zone of China

    Institute of Scientific and Technical Information of China (English)

    REN Wen; CHEN Fu-sheng; HU Xiao-fei; YU Ming-quan; FENG Xue

    2011-01-01

    Soil N transformations using the polyvinyl chloride (PVC)closed-top tube in situ incubation method were studied in Nanchang urban forests of the mid-subtropical region of China m different monthsof 2007.Four plots of 20 m × 20 m were established in four different plant communities that represented typical successional stages of forest development including shrubs,coniferous forest,mixed forest and broadleaved forest.Average concentrations of soil NH4+-N fiom January to December were not different among the four plant communities.The concentrations of soil NO3-N and mineral N,and the annual rates of ammonification,nitrification and net N-mineralization under the early successional shrub community and coniferous forest were generally lower than that of the late successional mixed and broad-leaved forests (p<0.05).Similar differences among the plant communities were also shown in the relative nitrification index (NH4+-N/NO3-N) and relative nitrification intensity (nitrification rate/net N-mineralization rate).The annual net N-mineralization rate was increased from younger to older plant communities,from 15.1 and 41.4 kg·ha-1·a-1 under the shrubs and coniferous forest communities to 98.0 and 112.9 kg·ha-1·a-1 under the mixed and broad-leaved forests,respectively.Moreover,the high annual nitrification rates (50-70 kg·ha-1·a-1) and its end product,NO3-N (2.4-3.8mg·kg-1),under older plant communities could increase the potential risk of N loss.Additionally,the temporal patterns of the different soil N variables mentioned above varied with different plant community due to the combined affects of natural biological processes associated with forest maturation and urbanization.Our results indicated that urban forests arc moving towards a state of"N saturation" (extremely nitrification rate and NO3-N content) as they mature.

  2. Expression of anti-tumor necrosis factor alpha (TNFα) single-chain variable fragment (scFv) in Spirodela punctata plants transformed with Agrobacterium tumefaciens.

    Science.gov (United States)

    Balaji, Parthasarathy; Satheeshkumar, P K; Venkataraman, Krishnan; Vijayalakshmi, M A

    2016-05-01

    Therapeutic antibodies against tumor necrosis factor alpha (TNFα) have been considered effective for some of the autoimmune diseases such as rheumatoid arthritis, Crohn's diseases, and so on. But associated limitations of the current therapeutics in terms of cost, availability, and immunogenicity have necessitated the need for alternative candidates. Single-chain variable fragment (scFv) can negate the limitations tagged with the anti-TNFα therapeutics to a greater extent. In the present study, Spirodela punctata plants were transformed with anti-TNFα through in planta transformation using Agrobacterium tumefaciens strain, EHA105. Instead of cefotaxime, garlic extract (1 mg/mL) was used to remove the agrobacterial cells after cocultivation. To the best of our knowledge, this report shows for the first time the application of plant extracts in transgenic plant development. 95% of the plants survived screening under hygromycin. ScFv cDNA integration in the plant genomic DNA was confirmed at the molecular level by PCR. The transgenic protein expression was followed up to 10 months. Expression of scFv was confirmed by immunodot blot. Protein expression levels of up to 6.3% of total soluble protein were observed. β-Glucuronidase and green fluorescent protein expressions were also detected in the antibiotic resistant plants. The paper shows the generation of transgenic Spirodela punctuata plants through in planta transformation.

  3. Effect of plant age, larval age, and fertilizer treatment on resistance of a cry1Ab-transformed aromatic rice to lepidopterous stem borers and foliage feeders.

    Science.gov (United States)

    Alinia, F; Ghareyazie, B; Rubia, L; Bennett, J; Cohen, M B

    2000-04-01

    The resistance of vegetative, booting, and flowering stage plants of a variety of an aromatic rice, Oryza sativa L., transformed with a Bacillus thuringiensis Berliner cry1Ab gene under control of the maize phosphoenolpyruvate carboxylase (PEPC) promoter was evaluated against four lepidopterous rice pests--the stem borers Chilo suppressalis (Walker) (Lepidoptera: Crambidae) and Scirpophaga incertulas (Walker) (Lepidoptera: Pyralidae), and the foliage feeders Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae) and Naranga aenescens Moore (Lepidoptera: Noctuidae). Plants of the cry1Ab-transformed line (no. 827) were more resistant to young larvae of S. incertulas, C. suppressalis, and C. medinalis than control plants at the vegetative stage but not at the flowering stage. Survival of 10-d-old stem borer larvae did not differ on cry1Ab plants and control plants at either the vegetative or flowering stage, but the development of 10-d-old C. suppressalis larvae was retarded on the vegetative stage cry1Ab plants. Immunological analysis also showed an apparent decline in Cry1Ab titer in leaf blades and leaf sheaths at the reproductive stage. In experiments comparing three fertilizer treatments (NPK, PK, and none), there was a significant interaction between fertilizer treatment and variety on larval survival only in whole-plant assays at booting stage with C. suppressalis. On cry1Ab plants, larval survival did not differ significantly among the three fertilizer levels, whereas on control plants survival was highest with the NPK treatment. cry1Ab plants tested at the sixth and seventh generations after transformation were more resistant than control plants to N. aenescens and C. suppressalis, respectively, suggesting that gene silencing will not occur in line 827. The results of the experiments are discussed in terms of resistance management for B. thuringiensis toxins in rice.

  4. Agrobacterium-mediated transformation of apple (Malus x domestica Borkh.): an assessment of factors affecting regeneration of transgenic plants.

    Science.gov (United States)

    De Bondt, A; Eggermont, K; Penninckx, I; Goderis, I; Broekaert, W F

    1996-03-01

    We have previously developed a protocol for efficient gene transfer and regeneration of transgenic calli following cocultivation of apple (cv. Jonagold) explants with Agrobacterium tumefaciens (De Bondt et al. 1994, Plant Cell Reports 13: 587-593). Now we report on the optimization of postcultivation conditions for efficient and reproducible regeneration of transgenic shoots from the apple cultivar Jonagold. Factors which were found to be essential for efficient shoot regeneration were the use of gelrite as a gelling agent and the use of the cytokinin-mimicing thidiazuron in the selective postcultivation medium. Improved transformation efficiencies were obtained by combining the hormones thidiazuron and zeatin and by using leaf explants from in vitro grown shoots not older than 4 weeks after multiplication. Attempts to use phosphinothricin acetyl transferase as a selectable marker were not successful. Using selection on kanamycin under optimal postcultivation conditions, about 2% of the leaf explants developed transgenic shoots or shoot clusters. The presence and expression of the transferred genes was verified by β-glucuronidase assays and Southern analysis. The transformation procedure has also been succesfully applied to several other apple cultivars.

  5. Attenuation of pharmaceuticals and their transformation products in a wastewater treatment plant and its receiving river ecosystem.

    Science.gov (United States)

    Aymerich, I; Acuña, V; Barceló, D; García, M J; Petrovic, M; Poch, M; Rodriguez-Mozaz, S; Rodríguez-Roda, I; Sabater, S; von Schiller, D; Corominas, Ll

    2016-09-01

    Pharmaceuticals are designed to improve human and animal health, but may also be a threat to freshwater ecosystems, particularly after receiving urban or wastewater treatment plant (WWTP) effluents. Knowledge on the fate and attenuation of pharmaceuticals in engineered and natural ecosystems is rather fragmented, and comparable methods are needed to facilitate the comprehension of those processes amongst systems. In this study the dynamics of 8 pharmaceuticals (acetaminophen, sulfapyridine, sulfamethoxazole, carbamazepine, venlafaxine, ibuprofen, diclofenac, diazepam) and 11 of their transformation products were investigated in a WWTP and the associated receiving river ecosystem. During 3 days, concentrations of these compounds were quantified at the influents, effluents, and wastage of the WWTP, and at different distances downstream the effluent at the river. Attenuation (net balance between removal and release from and to the water column) was estimated in both engineered and natural systems using a comparable model-based approach by considering different uncertainty sources (e.g. chemical analysis, sampling, and flow measurements). Results showed that pharmaceuticals load reduction was higher in the WWTP, but attenuation efficiencies (as half-life times) were higher in the river. In particular, the load of only 5 out of the 19 pharmaceuticals was reduced by more than 90% at the WWTP, while the rest were only partially or non-attenuated (or released) and discharged into the receiving river. At the river, only the load of ibuprofen was reduced by more than 50% (out of the 6 parent compounds present in the river), while partial and non-attenuation (or release) was observed for some of their transformation products. Linkages in the routing of some pharmaceuticals (venlafaxine, carbamazepine, ibuprofen and diclofenac) and their corresponding transformation products were also identified at both WWTP and river. Finally, the followed procedure showed that dynamic

  6. A study on advanced man-machine interface system for autonomous nuclear power plants

    Energy Technology Data Exchange (ETDEWEB)

    Matsuoka, Takeshi; Numano, Masayoshi; Someya, Minoru; Fukuto, Junji; Mitomo, Noboru; Miyazaki, Keiko; Sugasawa, Shinobu [Ship Research Inst., Mitaka, Tokyo (Japan)

    1997-01-01

    Research on Artificial Intelligence Systems for Nuclear Installations has been performed in cooperation with five research institutes (Ship Research Institute, Electrotechnical Laboratory, Japan Atomic Energy Research Institute, Power Reactor and Nuclear Fuel Development Corporation, The Institute of Physical and Chemical Research), from 1989 to 1994 as the Cross-over Research Group with the support of the Science and Technology Agency. Ship Research Institute has been carrying out the research on the Man-Machine Interface (MMI) system for autonomous nuclear power plants. This paper describes the concept of autonomous nuclear power plants, a plant simulator of an autonomous nuclear power plant, a contracted function model of a plant state, three-dimensional color graphic display of a plant state, and a function of automatic classification of plant states by the COBWEB method. A plant simulator has been developed by using the expert system G2 (Gensym Co.). The simulator generates plant process data at each component of a plant. This simulator models a pressurized water reactor and some examples of autonomous functions are incorporated. A contracted function model of a plant state has been produced at the main part of the MMI system based on plant process data from the simulator. The main purpose of the present study is to give the MMI system a function to identify the plant operational state, to update and revise the function model, and to expand a knowledge. A plant state is expressed in a three-dimensional graphic display which receives sensor values from the plant simulator and expresses the plant state in nearly real time speed. A research on the automatic classification of plant states has been also performed, which shows us the relations among different plant states. The study is being continued to the 2nd stage Cross-over Research from 1994, as the Study on Divers, Cooperative Intelligent System for Autonomous Plants. (J.P.N.)

  7. Enhanced transformation of TNT by Arabidopsis plants expressing an old yellow enzyme.

    Science.gov (United States)

    Zhu, Bo; Peng, Ri-He; Fu, Xiao-Yan; Jin, Xiao-Fen; Zhao, Wei; Xu, Jing; Han, Hong-Juan; Gao, Jian-Jie; Xu, Zhi-Sheng; Bian, Lin; Yao, Quan-Hong

    2012-01-01

    2,4,6-Trinitrotoluene (TNT) is released in nature from manufacturing or demilitarization facilities, as well as after the firing or detonation of munitions or leakage from explosive remnants of war. Environmental contamination by TNT is associated with human health risks, necessitating the development of cost-effective remediation techniques. The lack of affordable and effective cleanup technologies for explosives contamination requires the development of better processes. In this study, we present a system for TNT phytoremediation by overexpressing the old yellow enzyme (OYE3) gene from Saccharomyces cerevisiae. The resulting transgenic Arabidopsis plants demonstrated significantly enhanced TNT tolerances and a strikingly higher capacity to remove TNT from their media. The current work indicates that S. cerevisiae OYE3 overexpression in Arabidopsis is an efficient method for the phytoremoval and degradation of TNT. Our findings have the potential to provide a suitable remediation strategy for sites contaminated by TNT.

  8. Enhanced transformation of TNT by Arabidopsis plants expressing an old yellow enzyme.

    Directory of Open Access Journals (Sweden)

    Bo Zhu

    Full Text Available 2,4,6-Trinitrotoluene (TNT is released in nature from manufacturing or demilitarization facilities, as well as after the firing or detonation of munitions or leakage from explosive remnants of war. Environmental contamination by TNT is associated with human health risks, necessitating the development of cost-effective remediation techniques. The lack of affordable and effective cleanup technologies for explosives contamination requires the development of better processes. In this study, we present a system for TNT phytoremediation by overexpressing the old yellow enzyme (OYE3 gene from Saccharomyces cerevisiae. The resulting transgenic Arabidopsis plants demonstrated significantly enhanced TNT tolerances and a strikingly higher capacity to remove TNT from their media. The current work indicates that S. cerevisiae OYE3 overexpression in Arabidopsis is an efficient method for the phytoremoval and degradation of TNT. Our findings have the potential to provide a suitable remediation strategy for sites contaminated by TNT.

  9. The Next Generation Nuclear Plant Graphite Creep Experiment Irradiation in the Advanced Test Reactor

    Energy Technology Data Exchange (ETDEWEB)

    Blaine Grover

    2010-10-01

    The United States Department of Energy’s Next Generation Nuclear Plant (NGNP) Program will be irradiating six gas reactor graphite creep experiments in the Advanced Test Reactor (ATR) located at the Idaho National Laboratory (INL). The ATR has a long history of irradiation testing in support of reactor development and the INL has been designated as the United States Department of Energy’s lead laboratory for nuclear energy development. The ATR is one of the world’s premiere test reactors for performing long term, high flux, and/or large volume irradiation test programs. These graphite irradiations are being accomplished to support development of the next generation reactors in the United States. The graphite experiments will be irradiated over the next six to eight years to support development of a graphite irradiation performance data base on the new nuclear grade graphites now available for use in high temperature gas reactors. The goals of the irradiation experiments are to obtain irradiation performance data, including irradiation creep, at different temperatures and loading conditions to support design of the Next Generation Nuclear Plant (NGNP) Very High Temperature Gas Reactor, as well as other future gas reactors. The experiments will each consist of a single capsule that will contain six stacks of graphite specimens, with half of the graphite specimens in each stack under a compressive load, while the other half of the specimens will not be subjected to a compressive load during irradiation. The six stacks will have differing compressive loads applied to the top half of each pair of specimen stacks, while a seventh stack will not have a compressive load. The specimens will be irradiated in an inert sweep gas atmosphere with on-line temperature and compressive load monitoring and control. There will also be the capability of sampling the sweep gas effluent to determine if any oxidation or off-gassing of the specimens occurs during initial start-up of

  10. Development of advanced risk informed asset management tool based on system dynamics approach for nuclear power plant

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Gyoung Cheol

    2007-02-15

    In the competitive circumstance of electricity industry, the economic efficiency of electricity generation facility is the most important factor to increase their competitiveness. For nuclear power plant (NPP), safety is also an essential factor. Over fast several years, efforts for development of safety concerned and financial asset maximizing method, process and tools have been continued internationally and Risk-Informed Asset Management (RIAM) methodology is suggested by Electric Power Research Institute (EPRI). This RIAM methodology is expected to provide plant operators with a project prioritization and life cycle management planning tool for making long-term maintenance plans, guiding plant budgeting, and determining the sensitivity of a plant's economic risk to the reliability and availability of system, structure, and components (SSC), as well as other technical and economic parameters. The focus of this study is to develop model that help us to resource allocation, to find what effect such allocations on the plant economic and safety performance. Detailed research process for this goal is as follow; First step for development of advanced RIAM model is to review for current RIAM model of EPRI. This part describes the overall RIAM methodology including its conceptual model, implementation process, modular approach etc. Second step is to perform feasibility study for current EPRI's RIAM model with case study. This part shows the result of feasibility study for current RIAM method by case study and discussion for result. Finally, concept of Advanced RIAM model is developed based on system dynamics approach and parameter relationship is formulated. In advanced RIAM Model, Identification of scheduled maintenance effect on other parameters and the relationship between PM Activity and failure rate is most important factor. In this study, these relationships are formulated based on system dynamics approach. Creations of these modeling tool using Vensim

  11. Transformation of traditional knowledge of medicinal plants: the case of Tyroleans (Austria) who migrated to Australia, Brazil and Peru.

    Science.gov (United States)

    Pirker, Heidemarie; Haselmair, Ruth; Kuhn, Elisabeth; Schunko, Christoph; Vogl, Christian R

    2012-11-16

    In ethnobotanical research, the investigation into traditional knowledge of medicinal plants in the context of migration has been of increasing interest in recent decades since it is influenced and changed by new environmental and social conditions. It most likely undergoes transformation processes to match the different living circumstances in the new location. This study compares the traditional knowledge of medicinal plants held by Tyroleans - and their descendants - who emigrated to Australia, Brazil and Peru at different time scales. The study's findings allow a discussion of the complexities and dynamics that influence this knowledge within the context of long-distance migration. Information was obtained from 65 informants by free-listing, semi-structured interviews and non-participatory observation in Tyrol (Austria) and the migrants' countries: Australia, Brazil and Peru. The collected data was analysed using different quantitative approaches, including statistical tests, and compared between the countries of investigation. All respondents in all four investigation areas claimed that they had knowledge and made use of medicinal plants to treat basic ailments in their day-to-day lives. Informants made 1,139 citations of medicinal plants in total in free lists, which correspond to 164 botanical taxa (genus or species level) in Tyrol, 87 in Australia, 84 in Brazil and 134 in Peru. Of all the botanical taxa listed, only five (1.1%) were listed in all four countries under investigation. Agreement among informants within free lists was highest in Tyrol (17%), followed by Peru (12.2%), Australia (11.9%) and Brazil (11.2%). The proportion of agreement differs significantly between informants in Australia and Tyrol (p = 0.001), Brazil and Tyrol (p = 0.001) and Peru and Tyrol (p = 0.001) and is similar between informants in the migrant countries, as indicated by statistical tests. We recorded 1,286 use citations according to 744 different uses (Tyrol: 552, Australia

  12. Plant-wide modelling of phosphorus transformations in wastewater treatment systems: Impacts of control and operational strategies.

    Science.gov (United States)

    Solon, K; Flores-Alsina, X; Kazadi Mbamba, C; Ikumi, D; Volcke, E I P; Vaneeckhaute, C; Ekama, G; Vanrolleghem, P A; Batstone, D J; Gernaey, K V; Jeppsson, U

    2017-04-15

    The objective of this paper is to report the effects that control/operational strategies may have on plant-wide phosphorus (P) transformations in wastewater treatment plants (WWTP). The development of a new set of biological (activated sludge, anaerobic digestion), physico-chemical (aqueous phase, precipitation, mass transfer) process models and model interfaces (between water and sludge line) were required to describe the required tri-phasic (gas, liquid, solid) compound transformations and the close interlinks between the P and the sulfur (S) and iron (Fe) cycles. A modified version of the Benchmark Simulation Model No. 2 (BSM2) (open loop) is used as test platform upon which three different operational alternatives (A1, A2, A3) are evaluated. Rigorous sensor and actuator models are also included in order to reproduce realistic control actions. Model-based analysis shows that the combination of an ammonium ( [Formula: see text] ) and total suspended solids (XTSS) control strategy (A1) better adapts the system to influent dynamics, improves phosphate [Formula: see text] accumulation by phosphorus accumulating organisms (XPAO) (41%), increases nitrification/denitrification efficiency (18%) and reduces aeration energy (Eaeration) (21%). The addition of iron ( [Formula: see text] ) for chemical P removal (A2) promotes the formation of ferric oxides (XHFO-H, XHFO-L), phosphate adsorption (XHFO-H,P, XHFO-L,P), co-precipitation (XHFO-H,P,old, XHFO-L,P,old) and consequently reduces the P levels in the effluent (from 2.8 to 0.9 g P.m(-3)). This also has an impact on the sludge line, with hydrogen sulfide production ( [Formula: see text] ) reduced (36%) due to iron sulfide (XFeS) precipitation. As a consequence, there is also a slightly higher energy production (Eproduction) from biogas. Lastly, the inclusion of a stripping and crystallization unit (A3) for P recovery reduces the quantity of P in the anaerobic digester supernatant returning to the water line and allows

  13. Advanced numerical description of the behavior of 700 C steam power plant components

    Energy Technology Data Exchange (ETDEWEB)

    Maile, K. [Materialpruefungsanstalt, Univ. Stuttgart (Germany); Schmidt, K.; Roos, E.; Klenk, A.; Speicher, M.

    2009-07-01

    To make full use of the strength potential of new boiler materials like the new 9-11% Cr steels and nickel based alloys, taking into account their specific stress-strain relaxation behavior, new design methods are required in the design of today's power plants. Highly loaded components are approaching more and more the classical design limits with regard to critical wall thicknesses and the related tolerable thermal gradients, due to planed increases of steam parameters like steam pressure and steam temperature. ''Design by analysis'' can be realized by modern state of the art Numerical Finite Element (FE) simulation codes and in some cases by the use of user defined advanced inelastic material laws. These material laws have to be adjusted to specific material behavior of new boiler materials. To model the strain and stress situation in components under high temperature loading, a constitutive equation based on a Graham-Walles approach is used in this paper. Furthermore essential steps and recommendations to implement experimental data in the user defined subroutines and the subsequent integration of the subroutines in modern FE codes like ABAQUS trademark and ANSYS trademark are given. As an example, the results of FE simulations of components like hollow cylinders and waterwall like components made of Alloy 617 or 9-11% Cr steels are discussed and verified with experimental results. In a last step, the successful application of the developed creep equation will be demonstrated by calculating the creep strains and stress relaxation of a P92 steam header under constant loading. (orig.)

  14. Production of herbicide-resistant coffee plants (Coffea canephora P. via Agrobacterium tumefaciens-mediated transformation

    Directory of Open Access Journals (Sweden)

    Alessandra Ferreira Ribas

    2006-01-01

    Full Text Available Transgenic plants of Coffea canephora P. resistant to the herbicide ammonium glufosinate were regenerated from leaf explants after co-culture with Agrobacterium tumefaciens strain EHA105 harboring pCambia3301, a plasmid that contains the bar and the uidA genes both under control of 35S promoter. Direct somatic embryogenesis was induced on basal medium contained ¼ strength macro salts and half strength micro salts of MS medium, organic constituents of B5 medium and 30 g.L-1 sucrose supplemented with 5µM N6 - (2-isopentenyl-adenine (2-iP. Ten µM ammonium glufosinate was used for putative transgenic somatic embryos selection. Presence and integration of the bar gene were confirmed by PCR and Southern blot analysis. Selected transgenic coffee plants sprayed with up to 1600 mg.L-1 of FinaleTM, a herbicide containing glufosinate as the active ingredient, retained their pigmentation and continued to grow normally during ex vitro acclimation.Plantas transgênicas de Coffea canephora P resistentes ao herbicida glufosinato de amônio foram regeneradas a partir de explantes foliares co-cultivados com Agrobacterium tumefaciens EHA105 contendo o plasmídio pCambia3301 que contém os genes bar e uidA ambos sob controle do promotor 35S. Embriogênese somática direta foi induzida no meio contendo ¼ da concentração de macro, metade da concentração de micronutrientes do meio MS, constituintes orgânicos do meio B5 e 30 g.L-1 de sacarose suplementado com 5µM N6 - (2-isopentenil-adenina (2-iP e 10 µM de glufosinato de amônio para seleção de embriões transgênicos putativos. A presença e a integração do gene bar foram confirmados pelas análises de PCR e Southern blot. As plantas transgênicas selecionadas de café, pulverizadas com 1600 mg.L-1 do herbicida FinaleTM que contém glufosinato como ingrediente ativo, mantiveram a coloração e continuaram crescendo normalmente na aclimatação ex vitro.

  15. Development of an efficient transformation method by Agrobacterium tumefaciens and high throughput spray assay to identify transgenic plants for woodland strawberry (Fragaria vesca) using NPTII selection.

    Science.gov (United States)

    Pantazis, Christopher J; Fisk, Sarah; Mills, Kerri; Flinn, Barry S; Shulaev, Vladimir; Veilleux, Richard E; Dan, Yinghui

    2013-03-01

    KEY MESSAGE : We developed an efficient Agrobacterium -mediated transformation method using an Ac/Ds transposon tagging construct for F. vesca and high throughput paromomycin spray assay to identify its transformants for strawberry functional genomics. Genomic resources for Rosaceae species are now readily available, including the Fragaria vesca genome, EST sequences, markers, linkage maps, and physical maps. The Rosaceae Genomic Executive Committee has promoted strawberry as a translational genomics model due to its unique biological features and transformability for fruit trait improvement. Our overall research goal is to use functional genomic and metabolic approaches to pursue high throughput gene discovery in the diploid woodland strawberry. F. vesca offers several advantages of a fleshy fruit typical of most fruit crops, short life cycle (seed to seed in 12-16 weeks), small genome size (206 Mbb/C), small plant size, self-compatibility, and many seeds per plant. We have developed an efficient Agrobacterium tumefaciens-mediated strawberry transformation method using kanamycin selection, and high throughput paromomycin spray assay to efficiently identify transgenic strawberry plants. Using our kanamycin transformation method, we were able to produce up to 98 independent kanamycin resistant insertional mutant lines using a T-DNA construct carrying an Ac/Ds transposon Launchpad system from a single transformation experiment involving inoculation of 22 leaf explants of F. vesca accession 551572 within approx. 11 weeks (from inoculation to soil). Transgenic plants with 1-2 copies of a transgene were confirmed by Southern blot analysis. Using our paromomycin spray assay, transgenic F. vesca plants were rapidly identified within 10 days after spraying.

  16. [Genetic transformation of buckwheat ( Fagopyrum esculentum Moench ) with AtNHX1 gene and regeneration of salt-tolerant transgenic plants].

    Science.gov (United States)

    Cheng, Li-Hong; Zhang, Bo; Xu, Zi-Qin

    2007-01-01

    The Arabidopsis thaliana tonoplast Na+ /H+ antiporter gene, AtNHX1, was transferred into buckwheat by Agrobacterium-mediated method. Transgenic buckwheat plants were regenerated and selected on MS basal medium supplemented with 2.0mg/L 6-BA, 1.0mg/L KT, 0.lmg/L IAA, 50mg/L kanamycin and 500mg/L carbenicillin. 426 seedlings from 36 resistant calli originated from 864 explants (transformed about at 4.17 percentage) exhibited resistance to kanamycin. The transformants were confirmed by PCR, Southern blotting, RT-PCR and Northern blotting analysis. After stress treatment for 6 weeks with 200mmol/L NaCl, transgenic plants survived, while wild-type plants did not. After 3 days of stress treatment through different concentrations of NaCl, transgenic plants accumulated higher concentration of Na+ and proline than the control plants. However, the K+ concentration of transgenic plants declined in comparison with the control plants. Moreover, the rutin content of the roots, stems and leaves of transgenic buckwheat increased than those of the control plants. These results showed that it could be possible to improve the salt-tolerance of crops with genetic technology.

  17. Advanced design methodology for single and dual voltage wound core power transformers based on a particular finite element model

    Energy Technology Data Exchange (ETDEWEB)

    Tsili, Marina A.; Kladas, Antonios G. [Faculty of Electrical & amp; Computer Engineering, National Technical University of Athens, GR-15780, Athens (Greece); Georgilakis, Pavlos S. [Department of Production Engineering and Management, Technical University of Crete, GR-73100, Chania (Greece); Souflaris, Athanasios T.; Paparigas, Dimitris G. [Schneider Electric AE, Elvim Plant, GR-32011, Inofyta, Viotia (Greece)

    2006-06-15

    The paper presents an accurate and cost effective three-dimensional finite element model for the analysis and design of wound core, shell type, power transformers, focusing on the short-circuit impedance evaluation. The model efficiency lies on the detailed representation of the transformer geometry along with the adoption of a particular reduced scalar potential formulation enabling three-dimensional magnetostatic problem solution without prior source field calculation. Its accuracy is validated through local field measurements and through comparison of the calculated short-circuit impedance value with the measured one for several commercial wound core, shell type transformers. In such transformers, involving extensive winding parts out of the core window, the detailed representation of the transformer geometry, including the winding cooling ducts, provides accurate results for low densities of the three-dimensional finite element mesh, resulting to reduction of the required calculation time. The model is used in the development of a computational tool, which enables the automated and accurate transformer characteristics prediction, adopted to the manufacturing process. This tool has also been applied in the impedance calculation for different winding connections of dual voltage transformers, thus providing the information needed for the achievement of an accurate design and the enhancement of the manufacturer's ability to reduce design margins. The methodology presented in this paper has been incorporated in the design process of a transformer manufacturing industry. (author)

  18. The function of advanced treatment process in a drinking water treatment plant with organic matter-polluted source water.

    Science.gov (United States)

    Lin, Huirong; Zhang, Shuting; Zhang, Shenghua; Lin, Wenfang; Yu, Xin

    2017-04-01

    To understand the relationship between chemical and microbial treatment at each treatment step, as well as the relationship between microbial community structure in biofilms in biofilters and their ecological functions, a drinking water plant with severe organic matter-polluted source water was investigated. The bacterial community dynamics of two drinking water supply systems (traditional and advanced treatment processes) in this plant were studied from the source to the product water. Analysis by 454 pyrosequencing was conducted to characterize the bacterial diversity in each step of the treatment processes. The bacterial communities in these two treatment processes were highly diverse. Proteobacteria, which mainly consisted of beta-proteobacteria, was the dominant phylum. The two treatment processes used in the plant could effectively remove organic pollutants and microbial polution, especially the advanced treatment process. Significant differences in the detection of the major groups were observed in the product water samples in the treatment processes. The treatment processes, particularly the biological pretreatment and O3-biological activated carbon in the advanced treatment process, highly influenced the microbial community composition and the water quality. Some opportunistic pathogens were found in the water. Nitrogen-relative microorganisms found in the biofilm of filters may perform an important function on the microbial community composition and water quality improvement.

  19. [Advances in studies on growth metabolism and response mechanisms of medicinal plants under drought stress].

    Science.gov (United States)

    Si, Can; Zhang, Jun-Yi; Xu, Hu-Chao

    2014-07-01

    Drought stress exerts a considerable effect on growth, physiology and secondary metabolisms of the medicinal plants. It could inhabit the growth of the medicinal plants but promote secretion of secondary metabolites. Other researches indicated that the medicinal plants could depend on the ABA signaling pathway and secreting osmotic substances to resist the drought stress and reduce the damage by it. The article concludes the changes in growth, physiology, secondary metabolisms and response mechanisms of medicinal plants to drought stress that provides a theoretical basis for exploring the relationship between medicinal plants and drought stress.

  20. A review of methods for sensing the nitrogen status in plants: advantages, disadvantages and recent advances.

    Science.gov (United States)

    Muñoz-Huerta, Rafael F; Guevara-Gonzalez, Ramon G; Contreras-Medina, Luis M; Torres-Pacheco, Irineo; Prado-Olivarez, Juan; Ocampo-Velazquez, Rosalia V

    2013-08-16

    Nitrogen (N) plays a key role in the plant life cycle. It is the main plant mineral nutrient needed for chlorophyll production and other plant cell components (proteins, nucleic acids, amino acids). Crop yield is affected by plant N status. Thus, the optimization of nitrogen fertilization has become the object of intense research due to its environmental and economic impact. This article focuses on reviewing current methods and techniques used to determine plant N status. Kjeldahl digestion and Dumas combustion have been used as reference methods for N determination in plants, but they are destructive and time consuming. By using spectroradiometers, reflectometers, imagery from satellite sensors and digital cameras, optical properties have been measured to estimate N in plants, such as crop canopy reflectance, leaf transmittance, chlorophyll and polyphenol fluorescence. High correlation has been found between optical parameters and plant N status, and those techniques are not destructive. However, some drawbacks include chlorophyll saturation, atmospheric and soil interference, and the high cost of instruments. Electrical properties of plant tissue have been used to estimate quality in fruits, and water content in plants, as well as nutrient deficiency, which suggests that they have potential for use in plant N determination.

  1. Advances in RNA interference technology and its impact on nutritional improvement, disease and insect control in plants.

    Science.gov (United States)

    Katoch, Rajan; Thakur, Neelam

    2013-03-01

    This review highlights the advances in the knowledge of RNA interference (RNAi) and discusses recent progress on the functionality of different components RNAi machinery operating in the organisms. The silencing of genes by RNA interference has become the technology of choice for investigation of gene functions in different organisms. The refinement in the knowledge of the endogenous RNAi pathways in plants along with the development of new strategies and applications for the improvement of nutritional value of important agricultural crops through suppression of genes in different plants have opened new vistas for nutritional security. The improvement in the nutritional status of the plants and reduction in the level of toxins or antinutrients was desired for long, but the available technology was not completely successful in achieving the tissue specific regulation of some genes. In the recent years, a number of economically important crop plants have been tested successfully for improving plant nutritional value through metabolic engineering using RNAi. The implications of this technology for crop improvement programs, including nutritional enrichment, reduction of antinutrients, disease, and insect control have been successfully tested in variety of crops with commercial considerations. The enhancement of the nutraceutical traits for the desired health benefits in common crop plants through manipulation of gene expression has been elaborated in this article. The tremendous potential with RNAi technology is expected to revolutionize the modern agriculture for meeting the growing challenges is discussed.

  2. An Investigation for Arranging the Video Display Unit Information in a Main Control Room of Advanced Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Hsu, Chong Cheng; Yang, Chih Wei [Institute of Nuclear Energy Research, Atomic Energy Council, Taoyuan (China)

    2014-08-15

    Current digital instrumentation and control and main control room (MCR) technology has extended the capability of integrating information from numerous plant systems and transmitting needed information to operations personnel in a timely manner that could not be envisioned when previous generation plants were designed and built. A MCR operator can complete all necessary operating actions on the video display unit (VDU). It is extremely flexible and convenient for operators to select and to control the system display on the screen. However, a high degree of digitalization has some risks. For example, in nuclear power plants, failures in the instrumentation and control devices could stop the operation of the plant. Human factors engineering (HFE) approaches would be a manner to solve this problem. Under HFE considerations, there exists 'population stereotype' for operation. That is, the operator is used to operating a specific display on the specific VDU for operation. Under emergency conditions, there is possibility that the operator will response with this habit population stereotype, and not be aware that the current situation has already changed. Accordingly, the advanced nuclear power plant should establish the MCR VDU configuration plan to meet the consistent teamwork goal under normal operation, transient and accident conditions. On the other hand, the advanced nuclear power plant should establish the human factors verification and validation plan of the MCR VDU configuration to verify and validate the configuration of the MCR VDUs, and to ensure that the MCR VDU configuration allows the operator shift to meet the HFE consideration and the consistent teamwork goal under normal operation, transient and accident conditions. This paper is one of the HF V V plans of the MCR VDU configuration of the advanced nuclear power plant. The purpose of this study is to confirm whether the VDU configuration meets the human factors principles and the consistent

  3. Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants

    OpenAIRE

    Todaka, Daisuke; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko

    2015-01-01

    Advances have been made in the development of drought-tolerant transgenic plants, including cereals. Rice, one of the most important cereals, is considered to be a critical target for improving drought tolerance, as present-day rice cultivation requires large quantities of water and as drought-tolerant rice plants should be able to grow in small amounts of water. Numerous transgenic rice plants showing enhanced drought tolerance have been developed to date. Such genetically engineered plants ...

  4. The effects of regional insolation differences upon advanced solar thermal electric power plant performance and energy costs

    Science.gov (United States)

    Latta, A. F.; Bowyer, J. M.; Fujita, T.; Richter, P. H.

    1980-01-01

    The performance and cost of four 10 MWe advanced solar thermal electric power plants sited in various regions of the continental United States was studied. Each region has different insolation characteristics which result in varying collector field areas, plant performance, capital costs and energy costs. The regional variation in solar plant performance was assessed in relation to the expected rise in the future cost of residential and commercial electricity supplied by conventional utility power systems in the same regions. A discussion of the regional insolation data base is presented along with a description of the solar systems performance and costs. A range for the forecast cost of conventional electricity by region and nationally over the next several decades is given.

  5. Improving human reliability through better nuclear power plant system design: Program for advanced nuclear power studies. Progress report

    Energy Technology Data Exchange (ETDEWEB)

    Golay, M.W.

    1993-10-10

    The project on ``Development of a Theory of the Dependence of Human Reliability upon System Designs as a Means of Improving Nuclear Power Plant Performance`` was been undertaken in order to address the problem of human error in advanced nuclear power plant designs. Lack of a mature theory has retarded progress in reducing likely frequencies of human errors. Work being pursued in this project is to perform a set of experiments involving human subjects who are required to operate, diagnose and respond to changes in computer-simulated systems, relevant to those encountered in nuclear power plants, which are made to differ in complexity in a systematic manner. The computer program used to present the problems to be solved also records the response of the operator as it unfolds.

  6. Agrobacterium-transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer.

    Science.gov (United States)

    Cheng, X; Sardana, R; Kaplan, H; Altosaar, I

    1998-03-17

    Over 2,600 transgenic rice plants in nine strains were regenerated from >500 independently selected hygromycin-resistant calli after Agrobacterium-mediated transformation. The plants were transformed with fully modified (plant codon optimized) versions of two synthetic cryIA(b) and cryIA(c) coding sequences from Bacillus thuringiensis as well as the hph and gus genes, coding for hygromycin phosphotransferase and beta-glucuronidase, respectively. These sequences were placed under control of the maize ubiquitin promoter, the CaMV35S promoter, and the Brassica Bp10 gene promoter to achieve high and tissue-specific expression of the lepidopteran-specific delta-endotoxins. The integration, expression, and inheritance of these genes were demonstrated in R0 and R1 generations by Southern, Northern, and Western analyses and by other techniques. Accumulation of high levels (up to 3% of soluble proteins) of CryIA(b) and CryIA(c) proteins was detected in R0 plants. Bioassays with R1 transgenic plants indicated that the transgenic plants were highly toxic to two major rice insect pests, striped stem borer (Chilo suppressalis) and yellow stem borer (Scirpophaga incertulas), with mortalities of 97-100% within 5 days after infestation, thus offering a potential for effective insect resistance in transgenic rice plants.

  7. An in vivo Transient Expression System Can Be Applied for Rapid and Effective Selection of Artificial MicroRNA Constructs for Plant Stable Genetic Transformation

    Institute of Scientific and Technical Information of China (English)

    Basdeo Bhagwat; Ming Chi; Li Su; Haifeng Tang; Guiliang Tang; Yu Xiang

    2013-01-01

    The utility of artificial microRNAs (amiRNAs) to induce loss of gene function has been reported for many plant species,but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable.In this study,expressions of amiRNAs through the gene backbone of Arabidopsis miR 168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation.A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments.Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs.This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.

  8. Effectiveness of beneficial plant-microbe interactions under hypobaric and hypoxic conditions in an advanced life support system

    Science.gov (United States)

    MacIntyre, Olathe; Stasiak, Michael; Cottenie, Karl; Trevors, Jack; Dixon, Mike

    An assembled microbial community in the hydroponics solution of an advanced life support system may improve plant performance and productivity in three ways: (1) exclusion of plant pathogens from the initial community, (2) resistance to infection, and (3) plant-growth promotion. However, the plant production area is likely to have a hypobaric (low pressure) and hypoxic (low oxygen) atmosphere to reduce structural mass and atmosphere leakage, and these conditions may alter plant-microbe interactions. Plant performance and productivity of radish (Raphanus sativus L. cv. Cherry Bomb II) grown under hypobaric and hypoxic conditions were investigated at the University of Guelph's Controlled Environment Systems Research Facility. Changes in the microbial communities that routinely colonized the re-circulated nutrient solution, roots, and leaves of radishes in these experiments were quantified in terms of similarity in community composition, abundance of bacteria, and community diversity before and after exposure to hypobaric and hypoxic conditions relative to communities maintained at ambient growth conditions. The microbial succession was affected by extreme hypoxia (2 kPa oxygen partial pressure) while hypobaria as low as 10 kPa total pressure had little effect on microbial ecology. There were no correlations found between the physiological profile of these unintentional microbial communities and radish growth. The effects of hypobaric and hypoxic conditions on specific plant-microbe interactions need to be determined before beneficial gnotobiotic communities can be developed for use in space. The bacterial strains Tal 629 of Bradyrhizobium japonicum and WCS417 of Pseudomonas fluorescens, and the plant pathogen Fusarium oxysporum f. sp. raphani will be used in future experiments. B. japonicum Tal 629 promotes radish growth in hydroponics systems and P. fluorescens WCS417 induces systemic resistance to fusarium wilt (F. oxysporum f. sp. raphani) in radish under ambient

  9. Electric power plant technology. Generators, transformers, auxiliary installations, protective systems. Lecture notes. 5. rev. ed.; Elektrische Kraftwerkstechnik. Generatoren, Transformatoren, Eigenbedarfsanlagen, Schutzeinrichtungen. Vorlesungsskript

    Energy Technology Data Exchange (ETDEWEB)

    Wagner, Ulrich

    2009-07-01

    The lecture series on electric power plant engineering was initiated in 1969 by Prof. Helmut Schaefer, founder of the then department of power economy and power plant engineering. The lecture series was continuously updated in its contents and didactic approach, e.g. by integrating innovative power generation concepts like micro cogeneration plants and fuel cell systems. The focus is on the fundamentals and function of generators, main transformers, auxiliary installations and protective systems in power plant. Apart from the electrotechnical aspects, also aspects of mechanical engineering are gone into. Also discussed are energy conversion concepts fo renewable power generation plants like wind turbines and PV systems. This is the 5th, completely revised edition. It addresses primarily attendants of the lectures but may also serve as a textbook and reference manual for interested experts.

  10. Transformation of marine sediment to paddy soil: Primary marine, lacustrine, and land plant lipids

    Science.gov (United States)

    Mueller-Niggemann, Cornelia; Cao, Zhihong; Schwark, Lorenz

    2010-05-01

    More than fifty percent of the world's population feeds on rice. The continuous population increase and urban sprawl leads to an ever-increasing demand for new rice cultivation area, in particular China. For centuries suitable coastal areas in China have been exploited for land reclamation, i.e. conversion of coastal marine and lacustrine marshlands into rice paddy fields. Flooded rice paddies are considered one of the major biogenic sources of methane into the atmospheric. Methane is thought to be about 30 times more efficient as greenhouse gas, when compared to carbon dioxide. Overall, rice fields are assumed to contribute app. 10-25% to global CH4 production. It is thus paramount importance to study the effects of increasing rice cultivation and land reclamation in China. For global carbon cycle investigation, it is crucial whether paddy soils, due to their large extent and higher carbon turnover, serve as carbon (CO2) sinks or sources. Here we present results from a chronosequence study of paddy soils with different and well known starting dates of cultivation, in the Zhejiang province (Yangtze River delta) by land reclamation through the building of protective dikes over the past 2000 years. Two end members of natural sediments subjected to land reclamation, a marine tidal mudflat in the Yangtze delta and a coastal lake, represent the substrate on which the paddy soil evolution started. Dike systems were constructed 2000, 1000, 700, 300, 100, and 50 years before present. We are thus able to follow the evolution of rice paddy soils developed on marine sediments using eight well defined tie-points. This chronosequence is then used for assessing the relative proportion of primary marine or lacustrine organic matter preserved in present day soils and to identify the amount and composition of organic matter added since cultivation started. Paddy soil management introduces rice plants debris and exudates as well as rice-associated microbial biomass (covered in a

  11. Using an extractive Fourier transform infrared spectrometer for improving cleanroom air quality in a semiconductor manufacturing plant.

    Science.gov (United States)

    Li, Shou-Nan; Chang, Chin-Ta; Shih, Hui-Ya; Tang, Andy; Li, Alen; Chen, Yin-Yung

    2003-01-01

    A mobile extractive Fourier transform infrared (FTIR) spectrometer was successfully used to locate, identify, and quantify the "odor" sources inside the cleanroom of a semiconductor manufacturing plant. It was found that ozone (O(3)) gas with a peak concentration of 120 ppm was unexpectedly releasing from a headspace of a drain for transporting used ozonized water and that silicon tetrafluoride (SiF(4)) with a peak concentration of 3 ppm was off-gassed from silicon wafers after dry-etching processing. When the sources of the odors was pinpointed by the FTIR, engineering control measures were applied. For O(3) control, a water-sealed pipeline was added to prevent the O(3) gas (emitting from the ozonized water) from entering the mixing unit. A ventilation system also was applied to the mixing unit in case of O(3) release. For SiF(4) mitigation, before the wafer-out chamber was opened, N(2) gas with a flow rate of 150 L/min was used for 100 sec to purge the wafer-out chamber, and a vacuum system was simultaneously activated to pump away the purging N(2). The effectiveness of the control measures was assured by using the FTIR. In addition, the FTIR was used to monitor the potential hazardous gas emissions during preventative maintenance of the semiconductor manufacturing equipment.

  12. Assessment of nitrous oxide emission from cement plants: real data measured with both Fourier transform infrared and nondispersive infrared techniques.

    Science.gov (United States)

    Mosca, Silvia; Benedetti, Paolo; Guerriero, Ettore; Rotatori, Mauro

    2014-11-01

    Nitrous oxide (N2O) is the third most important greenhouse gas after carbon dioxide and methane, and contributes about 6% to the greenhouse effect. Nitrous oxide is a minor component of the atmosphere, and it is a thousand times less than carbon dioxide (CO2). Nevertheless, it is much more potent than CO2 and methane, owing to its long stay in the atmosphere of approximately 120 yr and the high global warmingpotential (GWP) of298 times that of CO2. Although greenhouse gases are natural in the atmosphere, human activities have changed the atmospheric concentrations. Most of the values of emission of nitrous oxide are still obtained by means ofemission factors and not actually measured; the lack ofreal data may result in an underestimation ofcurrent emissions. The emission factors used for the calculation of N2O can be obtained from the "Guidelines for the implementation of the national inventory of emissions" of the Intergovernmental Panel on Climate Change, which refer to all nations for the realization of their inventory. This study will present real data, measured in several Italian cement plants with different characteristics. The work also shows a comparison between N2O concentration measured with in situ-Fourier transform IR (FTIR) and the reference method EN ISO 21258 based on nondispersive IR (NDIR), in order to investigate the interfering compounds in the measurement with NDIR.

  13. Monitoring the spread of recombinant DNA from field plots with transgenic sugar beet plants by PCR and natural transformation of Pseudomonas stutzeri.

    Science.gov (United States)

    Meier, Petra; Wackernagel, Wilfried

    2003-06-01

    Previous studies had shown that recombinant DNA can be detected for several months in soil after the deposition of litter from transgenic (tg) plants. Here we show by PCR monitoring of field releases of tg sugar beet plants that during the growth of the plants the soil close to the plants and also plant material contains recombinant DNA, in the form of extracellular molecules. Surprisingly, the monitoring also revealed the presence of tg DNA in many field plots (30-70%) in which tg plants were never grown. These studies and the further monitoring during other tg sugar beet release experiments by PCR and a novel bioassay (measuring the transforming potential of recombinant DNA for Pseudomonas stutzeri) indicated that recombinant DNA was only detectable in the surface soil of field plots and their vicinity where flowering of the tg beet plants was allowed. Recombinant DNA was found in soil at a distance of 50 m from pollen-producing plants surrounded by a strip with hemp plants as a containment regime. It is concluded that recombinant DNA is deposited in soil during the growth of tg sugar beets and that a major mechanism of recombinant DNA spread in the environment is the dispersal of pollen which allows recombinant DNA to persist in the field plot for at least a year.

  14. ADVANCES IN THE APPLICATION OF REMOTE SENSING TO PLANT INCORPORATED PROTECTANT CROP MONITORING

    Science.gov (United States)

    Current forecasts call for significant increases to the plantings of transgenic corn in the United States for the 2007 growing season and beyond. Transgenic acreage approaching 80% of the total corn plantings could be realized by 2009. These conditions call for a new approach to ...

  15. Advances on plant-pathogen interactions from molecular toward systems biology perspectives.

    Science.gov (United States)

    Peyraud, Rémi; Dubiella, Ullrich; Barbacci, Adelin; Genin, Stéphane; Raffaele, Sylvain; Roby, Dominique

    2016-11-21

    In the past 2 decades, progress in molecular analyses of the plant immune system has revealed key elements of a complex response network. Current paradigms depict the interaction of pathogen-secreted molecules with host target molecules leading to the activation of multiple plant response pathways. Further research will be required to fully understand how these responses are integrated in space and time, and exploit this knowledge in agriculture. In this review, we highlight systems biology as a promising approach to reveal properties of molecular plant-pathogen interactions and predict the outcome of such interactions. We first illustrate a few key concepts in plant immunity with a network and systems biology perspective. Next, we present some basic principles of systems biology and show how they allow integrating multiomics data and predict cell phenotypes. We identify challenges for systems biology of plant-pathogen interactions, including the reconstruction of multiscale mechanistic models and the connection of host and pathogen models. Finally, we outline studies on resistance durability through the robustness of immune system networks, the identification of trade-offs between immunity and growth and in silico plant-pathogen co-evolution as exciting perspectives in the field. We conclude that the development of sophisticated models of plant diseases incorporating plant, pathogen and climate properties represent a major challenge for agriculture in the future.

  16. Production of novel biopolymers in plants: recent technological advances and future prospects.

    Science.gov (United States)

    Snell, Kristi D; Singh, Vijay; Brumbley, Stevens M

    2015-04-01

    The production of novel biopolymers in plants has the potential to provide renewable sources of industrial materials through agriculture. In this review we will highlight recent progress with plant-based production of polyhydroxyalkanoates (PHAs), silk, elastin, collagen, and cyanophycin with an emphasis on the synthesis of poly[(R)-3-hydroxybutyrate] (PHB), a renewable biodegradable PHA polymer with potential commercial applications in plastics, chemicals, and feed markets. Improved production of PHB has required manipulation of promoters driving expression of transgenes, reduction in activity of endogenous enzymes in competing metabolic pathways, insertion of genes to increase carbon flow to polymer, and basic plant biochemistry to understand metabolic limitations. These experiments have increased our understanding of carbon availability and partitioning in different plant organelles, cell types, and organs, information that is useful for the production of other novel molecules in plants.

  17. Advanced power plant concepts based on CFB combustion and combined cycles

    Energy Technology Data Exchange (ETDEWEB)

    Plass, L.; Beisswenger, H.; Anders, R. (Lurgi GmbH, Frankfurt am Main (Germany, F.R.). Coal and Energy Division)

    1990-01-01

    The paper describes several power plants employing circulating fluidized bed (CFB) technology. The Moabit power plant of BEWAG (Berliner Kraft und Licht Aktiengesellschaft) supplies electric power and steam for district heating. The Charbonnages de France group has built a 367 t/h CFB boiler with reheat at the Emile Huchet power station in Carling, Lorraine France. This uses dry fuel but operation with slurry feed is scheduled soon. The 150 MWe CFB boiler operated by Texas New Mexico Power Company is the largest CFB boiler worldwide. Salient features of boiler design for these plants are discussed. All meet requirements for low SO{sub 2} and NO{sub x} and particulate emission but have been developed to the full. Improved efficiency and low emission levels can be met by combined cycle power plants employing coal gasification. Demonstration plants for example using the BGL-fixed bed gasifier are mentioned. 1 ref., 13 figs., 7 tabs.

  18. Micromechanics of plastic deformation and phase transformation in a three-phase TRIP-assisted advanced high strength steel: Experiments and modeling

    Science.gov (United States)

    Srivastava, Ankit; Ghassemi-Armaki, Hassan; Sung, Hyokyung; Chen, Peng; Kumar, Sharvan; Bower, Allan F.

    2015-05-01

    The micromechanics of plastic deformation and phase transformation in a three-phase advanced high strength steel are analyzed both experimentally and by microstructure-based simulations. The steel examined is a three-phase (ferrite, martensite and retained austenite) quenched and partitioned sheet steel with a tensile strength of ~980 MPa. The macroscopic flow behavior and the volume fraction of martensite resulting from the austenite-martensite transformation during deformation were measured. In addition, micropillar compression specimens were extracted from the individual ferrite grains and the martensite particles, and using a flat-punch nanoindenter, stress-strain curves were obtained. Finite element simulations idealize the microstructure as a composite that contains ferrite, martensite and retained austenite. All three phases are discretely modeled using appropriate crystal plasticity based constitutive relations. Material parameters for ferrite and martensite are determined by fitting numerical predictions to the micropillar data. The constitutive relation for retained austenite takes into account contributions to the strain rate from the austenite-martensite transformation, as well as slip in both the untransformed austenite and product martensite. Parameters for the retained austenite are then determined by fitting the predicted flow stress and transformed austenite volume fraction in a 3D microstructure to experimental measurements. Simulations are used to probe the role of the retained austenite in controlling the strain hardening behavior as well as internal stress and strain distributions in the microstructure.

  19. Advanced Micromechanical Model for Transformation-Induced Plasticity Steels with Application of In-Situ High-Energy X-Ray Diffraction Method

    Science.gov (United States)

    Choi, K. S.; Liu, W. N.; Sun, X.; Khaleel, M. A.; Ren, Y.; Wang, Y. D.

    2008-12-01

    Compared to other advanced high-strength steels, transformation-induced plasticity (TRIP) steels exhibit better ductility at a given strength level and can be used to produce complicated automotive parts. This enhanced formability comes from the transformation of retained austenite to martensite during plastic deformation. In this study, as a first step in predicting optimum processing parameters in TRIP steel productions, a micromechanical finite element model is developed based on the actual microstructure of a TRIP 800 steel. The method uses a microstructure-based representative volume element (RVE) to capture the complex deformation behavior of TRIP steels. The mechanical properties of the constituent phases of the TRIP 800 steel and the fitting parameters describing the martensite transformation kinetics are determined using the synchrotron-based in-situ high-energy X-ray diffraction (HEXRD) experiments performed under a uniaxial tensile deformation. The experimental results suggest that the HEXRD technique provides a powerful tool for characterizing the phase transformation behavior and the microstress developed due to the phase-to-phase interaction of TRIP steels during deformation. The computational results suggest that the response of the RVE well represents the overall macroscopic behavior of the TRIP 800 steel under deformation. The methodology described in this study may be extended for studying the effects of the various processing parameters on the macroscopic behaviors of TRIP steels.

  20. PILOT-AND FULL-SCALE DEMONSTRATION OF ADVANCED MERCURY CONTROL TECHNOLOGIES FOR LIGNITE-FIRED POWER PLANTS

    Energy Technology Data Exchange (ETDEWEB)

    Steven A. Benson; Charlene R. Crocker; Kevin C. Galbreath; Jay R. Gunderson; Michael J. Holmes; Jason D. Laumb; Jill M. Mackenzie; Michelle R. Olderbak; John H. Pavlish; Li Yan; Ye Zhuang

    2005-02-01

    The overall objective of the project was to develop advanced innovative mercury control technologies to reduce mercury emissions by 50%-90% in flue gases typically found in North Dakota lignite-fired power plants at costs from one-half to three-quarters of current estimated costs. Power plants firing North Dakota lignite produce flue gases that contain >85% elemental mercury, which is difficult to collect. The specific objectives were focused on determining the feasibility of the following technologies: Hg oxidation for increased Hg capture in dry scrubbers, incorporation of additives and technologies that enhance Hg sorbent effectiveness in electrostatic precipitators (ESPs) and baghouses, the use of amended silicates in lignite-derived flue gases for Hg capture, and the use of Hg adsorbents within a baghouse. The approach to developing Hg control technologies for North Dakota lignites involved examining the feasibility of the following technologies: Hg capture upstream of an ESP using sorbent enhancement, Hg oxidation and control using dry scrubbers, enhanced oxidation at a full-scale power plant using tire-derived fuel and oxidizing catalysts, and testing of Hg control technologies in the Advanced Hybrid{trademark} filter.

  1. [Construction of plant expression vectors with PMI gene as selection marker and their utilization in transformation of Salvia miltiorrhiza f. alba].

    Science.gov (United States)

    Tao, Ru; Zhang, You-Can; Fang, Qian; Shi, Ren-Jiu; Li, Yan-Ling; Huang, Lu-Qi; Hao, Gang-Ping

    2014-04-01

    To construct plant expression pCAMBIA1301-PMI by substituting PMI for hygromycin resistance gene in pCAMBIA1301 and obtain transgenic Salvia miltiorrhiza f. alba using PMI-mannose selection system. The 6-phosphomannose isomerase gene (PMI) of Escherichia coli was amplified by PCR. Sequence analysis showed that it shared 100% amino acids identities with the sequences of PMI genes isolates reported in the NCBI. Based on pCAMBIA1305, the plant expression pCAMBIA1305-PMI was constructed successfully by substituting PMI for hygromycin resistance gene in pCAMBIA1305. pCAMBIA1305-PMI was transformed into Agrobacterium tumefaciens LBA4404, and then the leaves of S. miltiorrhiza f. alba were inoculated in LBA4404 with pCAMBIA1305-PMI. Plant expression pCAMBIA1301-PMI was successfully constructed and the leaves of S. miltiorrhiza f. alba inoculated in LBA4404 with pCAMBIA1305-PMI were selected on medium supplemented with a combination of 20 g x L(-1) mannose and 10 g x L(-1) sucrose as a carbon source. The transformation efficiency rate was 23.7%. Genetic transformation was confirmed by PCR, indicating that a new method for obtaining transgenic S. miltiorrhiza f. alba plants was developed using PMI-mannose selection system.

  2. Advances in seed conservation of wild plant species: a review of recent research

    National Research Council Canada - National Science Library

    Hay, Fiona R; Probert, Robin J

    2013-01-01

    .... Seed banking is now widely used for the ex situ conservation of wild plant species. Many seed banks that conserve wild species broadly follow international genebank guidelines for seed collection, processing, storage, and management...

  3. Recent advances in the analysis of metal hyperaccumulation and hypertolerance in plants using proteomics

    National Research Council Canada - National Science Library

    Dalcorso, Giovanni; Fasani, Elisa; Furini, Antonella

    2013-01-01

    Hyperaccumulator/hypertolerant plant species have evolved strategies allowing them to grow in metal-contaminated soils, where they accumulate high concentrations of heavy metals in their shoots without signs of toxicity...

  4. Operational Evaluation of the Root Modules of the Advanced Plant Habitat

    Science.gov (United States)

    Monje, O.

    2014-01-01

    Photosynthetic and growth data were collected on APH Root Module. Described Stand pipe system for active moisture control. Tested germination in wicks. Evaluated EC-5 moisture sensors. Demonstrated that Wheat plants can grow in the APH Root Module.

  5. Advances in biotechnology and informatics to link variation in the genome to phenotypes in plants and animals.

    Science.gov (United States)

    Appels, R; Barrero, R; Bellgard, M

    2013-03-01

    Advances in our understanding of genome structure provide consistent evidence for the existence of a core genome representing species classically defined by phenotype, as well as conditionally dispensable components of the genome that shows extensive variation between individuals of a given species. Generally, conservation of phenotypic features between species reflects conserved features of the genome; however, this is evidently not necessarily always the case as demonstrated by the analysis of the tunicate chordate Oikopleura dioica. In both plants and animals, the methylation activity of DNA and histones continues to present new variables for modifying (eventually) the phenotype of an organism and provides for structural variation that builds on the point mutations, rearrangements, indels, and amplification of retrotransposable elements traditionally considered. The translation of the advances in the structure/function analysis of the genome to industry is facilitated through the capture of research outputs in "toolboxes" that remain accessible in the public domain.

  6. Recent advances in plant biotechnology and genetic engineering for production of secondary metabolites.

    Science.gov (United States)

    Sheludko, Y V

    2010-01-01

    For a long time people are using plants not only as crop cultures but also for obtaining of various chemicals. Currently plants remain one of the most important and essential sources of biologically active compounds in spite of progress in chemical or microbial synthesis. In our review we compare potentials and perspectives of modern genetic engineering approaches for pharmaceutical biotechnology and give examples of actual biotechnological systems used for production of several promising natural compounds: artemisinin, paclitaxel and scopolamine.

  7. [Research advances in drought resistance and heavy metals tolerance of transgenic plant].

    Science.gov (United States)

    Zhang, Yu-Xiu; Xu, Jin; Wang, Xiao; Chai, Tuan-Yao

    2007-07-01

    Drought and heavy metals contamination greatly affect plant growth, while the cloning and function analysis of stress-inducible genes provide an effective approach to improve the stress tolerance and yield of crops by genetic engineering. The expression of LEA (late embryogenesis abundant protein) gene could be induced by various stresses such as drought, high salinity, cold, and heavy metals. The study on transgenic plants showed that LEA could increase plant tolerance to water stress, had ion-binding activity, and acted as an antioxidant under abiotic stresses. Aquaporins largely presented in plasma membrane and vacuolar membrane, and played a key role in root water uptake and transportation both at cellular and at whole plant level. The expression of aquaporins was up-regulated in response to drought and salinity, and conferred the water stress tolerance in plant. Cation-efflux transport proteins were involved in the absorption, transportation, and accumulation of heavy metals in plants. All of the proteins mentioned above could have potential applied profits on improving the biological water-saving, drought resistance, and heavy metals tolerance of lawny grass.

  8. [Advances in studies of absorption and utilization of amino acids by plants: A review].

    Science.gov (United States)

    Cao, Xiao-chuang; Wu, Liang-huan; Ma, Qing-xu; Jin, Qian-yu

    2015-03-01

    Plant can directly take up the intact amino acids, thus bypass the microbial mineralization of organic nitrogen. As an excellent carbon and nitrogen source, there exists competition for amino acid absorption between plant roots.and soil microorganisms. And the total flux of amino acids in soil may be enormous due to the extensive sources and short half-life. Studies on amino acid nitrogen nutritional contribution for plant by the technique of nitrogen isotopic tracer, has become a research topic in recent years ,which will help us better understand the principle of soil fertility. This paper summarized the recent researches on amino acid morphological characteristics in soil and its metabolic mechanism and nitrogen nutritional contribution for plant in different ecosystems, and discussed the present status and development trend of the amino acid circulation mechanism in the plant-soil-microorganism ecosystem and its bioavailability for plant. Finally, the topics of environmental regulating mechanism of amino acid bioavailability, amino acid carbon-nitrogen metabolism, and how to improve the field organic nitrogen management were all the core issues to be resolved.

  9. Recent Advances in the Role of the Elongator Complex in Plant Physiology and tRNA Modiifcation:A Review

    Institute of Scientific and Technical Information of China (English)

    YAN Xu; JIN Xiao-huan; WANG You-mei; ZHENG Bo; CHEN Peng

    2014-01-01

    The Elongator complex is a multifunction protein complex which has been shown to be involved in transcriptional elongation, DNA replication and repair, tubulin and histone acetylation, gene silencing and tranfer RNA uridine modiifcation. The composition of the Elongator complex is found to be highly conserved in eukaryotes, protein homologs of various subunits have been identiifed in fungi, plant, animal, and human. Remarkably, mutation in genes encoding the Elongator complex structural components all results in defects of transfer RNA wobble uridine modiifcation, and this function of the Elongator complex is also conserved in eukaryotes. The Elongator complex mutants in higher plants have pleiotropic phenotypes including defects in vegetative growth, abiscisic acid hypersensitivity, elevated tolerance to drought and oxidative stress. What is the relationship between the Elongator complex’s function in nucleoside modiifcation and its activity in other cellular pathways? This review summarizes the recent advances in study of function of the Elongator complex, in the aspects of cell physiology and molecular biology.

  10. Advanced analytical techniques for the extraction and characterization of plant-derived essential oils by gas chromatography with mass spectrometry.

    Science.gov (United States)

    Waseem, Rabia; Low, Kah Hin

    2015-02-01

    In recent years, essential oils have received a growing interest because of the positive health effects of their novel characteristics such as antibacterial, antifungal, and antioxidant activities. For the extraction of plant-derived essential oils, there is the need of advanced analytical techniques and innovative methodologies. An exhaustive study of hydrodistillation, supercritical fluid extraction, ultrasound- and microwave-assisted extraction, solid-phase microextraction, pressurized liquid extraction, pressurized hot water extraction, liquid-liquid extraction, liquid-phase microextraction, matrix solid-phase dispersion, and gas chromatography (one- and two-dimensional) hyphenated with mass spectrometry for the extraction through various plant species and analysis of essential oils has been provided in this review. Essential oils are composed of mainly terpenes and terpenoids with low-molecular-weight aromatic and aliphatic constituents that are particularly important for public health.

  11. Conceptual design of advanced central receiver power systems sodium-cooled receiver concept. Volume 4. Commercial and pilot plant cost data. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1979-03-01

    This volume of the advanced central receiver final report presents the cost data using the cost breakdown structure identified in the preliminary specification. Cost summaries are presented in the following sections for the 100-MWe and 281-MWe commercial plant and a 10-MWe pilot plant. Cost substantiation data for this volume are presented in the appendices. Other cost summary data include Nth plant data for the 100-MWe and 281-MWe commercial plants, and a summary for the alternative concept air-rock storage system. The main description of the plant costing technique occurs as part of Section II for the 100-MWe baseline concept.

  12. Conceptual design of advanced central receiver power systems sodium-cooled receiver concept. Volume 4. Commercial and pilot plant cost data. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1979-03-01

    This volume of the advanced central receiver final report presents the cost data using the cost breakdown structure identified in the preliminary specification. Cost summaries are presented in the following sections for the 100-MWe and 281-MWe commercial plant and a 10-MWe pilot plant. Cost substantiation data for this volume are presented in the appendices. Other cost summary data include Nth plant data for the 100-MWe and 281-MWe commercial plants, and a summary for the alternative concept air-rock storage system. The main description of the plant costing technique occurs as part of Section II for the 100-MWe baseline concept.

  13. 植物类胡萝卜素研究进展%Advances of Carotenoid Research in Plants

    Institute of Scientific and Technical Information of China (English)

    侯耀兵; 康保珊; 黄进勇

    2009-01-01

    The paper reviewed the advances of research on carotenoid structure,enzyme and genes regulating carotenoid biosynthetic pathway in plant,especially in watermelon. The challenges and prospect of carotenoid research are also discussed.%结合西瓜介绍植物中类胡萝卜素的结构、生物合成途径中的酶及基因、合成调控研究进展,提出目前研究存在的问题,并展望未来的研究方向.

  14. Advanced development in phytochemicals analysis of medicine and food dual purposes plants used in China (2011-2014).

    Science.gov (United States)

    Zhao, Jing; Ge, Li-Ya; Xiong, Wei; Leong, Fong; Huang, Lu-Qi; Li, Shao-Ping

    2016-01-08

    In 2011, we wrote a review for summarizing the phytochemical analysis (2006-2010) of medicine and food dual purposes plants used in China (Zhao et al., J. Chromatogr. A 1218 (2011) 7453-7475). Since then, more than 750 articles related to their phytochemical analysis have been published. Therefore, an updated review for the advanced development (2011-2014) in this topic is necessary for well understanding the quality control and health beneficial phytochemicals in these materials, as well as their research trends.

  15. Inland Treatment of the Brine Generated from Reverse Osmosis Advanced Membrane Wastewater Treatment Plant Using Epuvalisation System

    Science.gov (United States)

    Qurie, Mohannad; Abbadi, Jehad; Scrano, Laura; Mecca, Gennaro; Bufo, Sabino A.; Khamis, Mustafa; Karaman, Rafik

    2013-01-01

    The reverse osmosis (RO) brine generated from the Al-Quds University wastewater treatment plant was treated using an epuvalisation system. The advanced integrated wastewater treatment plant included an activated sludge unit, two consecutive ultrafiltration (UF) membrane filters (20 kD and 100 kD cutoffs) followed by an activated carbon filter and a reverse osmosis membrane. The epuvalisation system consisted of salt tolerant plants grown in hydroponic channels under continuous water flowing in a closed loop system, and placed in a greenhouse at Al-Quds University. Sweet basil (Ocimum basilicum) plants were selected, and underwent two consecutive hydroponic flowing stages using different brine-concentrations: an adaptation stage, in which a 1:1 mixture of brine and fresh water was used; followed by a functioning stage, with 100% brine. A control treatment using fresh water was included as well. The experiment started in April and ended in June (2012). At the end of the experiment, analysis of the effluent brine showed a remarkable decrease of electroconductivity (EC), PO43−, chemical oxygen demand (COD) and K+ with a reduction of 60%, 74%, 70%, and 60%, respectively, as compared to the influent. The effluent of the control treatment showed 50%, 63%, 46%, and 90% reduction for the same parameters as compared to the influent. Plant growth parameters (plant height, fresh and dry weight) showed no significant difference between fresh water and brine treatments. Obtained results suggest that the epuvalisation system is a promising technique for inland brine treatment with added benefits. The increasing of channel number or closed loop time is estimated for enhancing the treatment process and increasing the nutrient uptake. Nevertheless, the epuvalisation technique is considered to be simple, efficient and low cost for inland RO brine treatment. PMID:23823802

  16. Advanced design and economic considerations for commercial geothermal power plants at Heber and Niland, California. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1977-10-01

    Two separate studies, involving advanced design and economic considerations for commercial geothermal power plants using liquid-dominated hydrothermal resources, are presented. In the first study, the effects on design, capital cost, and bus bar electric energy production cost caused by an anticipated decline in available geothermal fluid temperature over the lifetime of power plants are described. A two-stage, flashed-steam energy conversion process was used for the conceptual design of the power plants, which operate from the moderate-temperature, low-salinity reservoir at Heber, California. Plants with net capacities of 50, 100, and 200 MWe (net) were investigated. The results show that it is important to include provision for geothermal fluid temperature decline in the design of power plants to prevent loss of electric energy production capability and to reduce bus bar electric energy costs. In the second study, the technical, economic, and environmental effects of adding regeneration to a 50 MWe (net) power plant employing the multistage-flash/binary process are described. Regeneration is potentially attractive because it recovers waste heat from the turbine exhaust and uses it in the power cycle. However, the pressure drop caused by the introduction of the regenerator decreases the turbine expansion and thus decreases system performance. An innovative approach was taken in the design of the regenerator, which minimized the expected performance degradation of the turbine. The result was that the performance, capital cost, and bus bar electric energy production cost are nearly the same for the processes with and without regeneration. On the other hand, the addition of regeneration has the environmental benefits of substantially reducing heat rejection to the atmosphere and cooling tower makeup and blowdown water requirements. It also increases the temperature of the brine returned to the field for reinjection.

  17. Inland Treatment of the Brine Generated from Reverse Osmosis Advanced Membrane Wastewater Treatment Plant Using Epuvalisation System

    Directory of Open Access Journals (Sweden)

    Mohannad Qurie

    2013-07-01

    Full Text Available The reverse osmosis (RO brine generated from the Al-Quds University wastewater treatment plant was treated using an epuvalisation system. The advanced integrated wastewater treatment plant included an activated sludge unit, two consecutive ultrafiltration (UF membrane filters (20 kD and 100 kD cutoffs followed by an activated carbon filter and a reverse osmosis membrane. The epuvalisation system consisted of salt tolerant plants grown in hydroponic channels under continuous water flowing in a closed loop system, and placed in a greenhouse at Al-Quds University. Sweet basil (Ocimum basilicum plants were selected, and underwent two consecutive hydroponic flowing stages using different brine-concentrations: an adaptation stage, in which a 1:1 mixture of brine and fresh water was used; followed by a functioning stage, with 100% brine. A control treatment using fresh water was included as well. The experiment started in April and ended in June (2012. At the end of the experiment, analysis of the effluent brine showed a remarkable decrease of electroconductivity (EC, PO43−, chemical oxygen demand (COD and K+ with a reduction of 60%, 74%, 70%, and 60%, respectively, as compared to the influent. The effluent of the control treatment showed 50%, 63%, 46%, and 90% reduction for the same parameters as compared to the influent. Plant growth parameters (plant height, fresh and dry weight showed no significant difference between fresh water and brine treatments. Obtained results suggest that the epuvalisation system is a promising technique for inland brine treatment with added benefits. The increasing of channel number or closed loop time is estimated for enhancing the treatment process and increasing the nutrient uptake. Nevertheless, the epuvalisation technique is considered to be simple, efficient and low cost for inland RO brine treatment.

  18. Inland treatment of the brine generated from reverse osmosis advanced membrane wastewater treatment plant using epuvalisation system.

    Science.gov (United States)

    Qurie, Mohannad; Abbadi, Jehad; Scrano, Laura; Mecca, Gennaro; Bufo, Sabino A; Khamis, Mustafa; Karaman, Rafik

    2013-07-03

    The reverse osmosis (RO) brine generated from the Al-Quds University wastewater treatment plant was treated using an epuvalisation system. The advanced integrated wastewater treatment plant included an activated sludge unit, two consecutive ultrafiltration (UF) membrane filters (20 kD and 100 kD cutoffs) followed by an activated carbon filter and a reverse osmosis membrane. The epuvalisation system consisted of salt tolerant plants grown in hydroponic channels under continuous water flowing in a closed loop system, and placed in a greenhouse at Al-Quds University. Sweet basil (Ocimum basilicum) plants were selected, and underwent two consecutive hydroponic flowing stages using different brine-concentrations: an adaptation stage, in which a 1:1 mixture of brine and fresh water was used; followed by a functioning stage, with 100% brine. A control treatment using fresh water was included as well. The experiment started in April and ended in June (2012). At the end of the experiment, analysis of the effluent brine showed a remarkable decrease of electroconductivity (EC), PO43-, chemical oxygen demand (COD) and K+ with a reduction of 60%, 74%, 70%, and 60%, respectively, as compared to the influent. The effluent of the control treatment showed 50%, 63%, 46%, and 90% reduction for the same parameters as compared to the influent. Plant growth parameters (plant height, fresh and dry weight) showed no significant difference between fresh water and brine treatments. Obtained results suggest that the epuvalisation system is a promising technique for inland brine treatment with added benefits. The increasing of channel number or closed loop time is estimated for enhancing the treatment process and increasing the nutrient uptake. Nevertheless, the epuvalisation technique is considered to be simple, efficient and low cost for inland RO brine treatment.

  19. Advanced monitoring and supervision of biological treatment of complex dairy effluents in a full-scale plant.

    Science.gov (United States)

    Carrasco, Eugenio F; Omil, Francisco; Garrido, Juan M; Arrojo, Belén; Méndez, Ramón

    2004-01-01

    The operation of a wastewater treatment plant treating effluents from a dairy laboratory was monitored by an advanced system. This plant comprises a 12 m(3) anaerobic filter (AF) reactor and a 28 m(3) sequential batch reactor (SBR) coupled in series and is equipped with the following on-line measurement devices: biogas flow meter, feed and recycling flow meters, temperature sensor, dissolved oxygen analyzer, and redox meter. Other parameters such as chemical oxygen demand (COD), volatile fatty acids (VFA), etc. were determined off-line. The plant has been in operation for 634 days, the influent flow rate being 6-8 m(3)/d. COD concentration of the influent ranged between 8 and 12 kg COD/m(3), resulting in COD values in the effluent around 50-200 mg/L. The behavior of the system was studied using the set of measurements collected by the data acquisition program especially developed for this purpose. Monitoring of variables such as anaerobic reactor temperature permitted the detection and prevention of several failures such as temperature shocks in the AF reactor. Besides, off-line measurements such as the alkalinity or the VFA content, together with the on-line measurements, provided immediate information about the state of the plant and the detection of several anomalies, such as organic overloads in the SBR, allowing the implementation of several fast control actions.

  20. Agrobacterium-mediated genetic transformation and regeneration of transgenic plants using leaf midribs as explants in ramie [Boehmeria nivea (L.) Gaud].

    Science.gov (United States)

    An, Xia; Wang, Bo; Liu, Lijun; Jiang, Hui; Chen, Jie; Ye, Shengtuo; Chen, Leiyu; Guo, Pingan; Huang, Xing; Peng, Dingxiang

    2014-05-01

    In this study, leaf midribs, the elite explants, were used for the first time to develop an efficient regeneration and transformation protocol for ramie [Boehmeria nivea (L.) Gaud.] via Agrobacterium-mediated genetic transformation. Sensitivity of leaf midribs regeneration to kanamycin was evaluated, which showed that 40 mg l(-1) was the optimal concentration needed to create the necessary selection pressure. Factors affecting the ramie transformation efficiency were evaluated, including leaf age, Agrobacterium concentration, length of infection time for the Agrobacterium solution, acetosyringone concentration in the co-cultivation medium, and the co-cultivation period. The midrib explants from 40-day-old in vitro shoots, an Agrobacterium concentration at OD600 of 0.6, 10-min immersion in the bacteria solution, an acetosyringone concentration of 50 mg l(-1) in the co-cultivation medium and a 3-day co-cultivation period produced the highest efficiencies of regeneration and transformation. In this study, the average transformation rate was 23.25%. Polymerase chain reactions using GUS and NPTII gene-specific primers, Southern blot and histochemical GUS staining analyses further confirmed that the transgene was integrated into the ramie genome and expressed in the transgenic ramie. The establishment of this system of Agrobacterium-mediated genetic transformation and regeneration of transgenic plants will be used not only to introduce genes of interest into the ramie genome for the purpose of trait improvement, but also as a common means of testing gene function by enhancing or inhibiting the expression of target genes.

  1. Advances in the application of plant growth-promoting rhizobacteria in phytoremediation of heavy metals.

    Science.gov (United States)

    Tak, Hamid Iqbal; Ahmad, Faheem; Babalola, Olubukola Oluranti

    2013-01-01

    In this review, we briefly describe the biological application of PGPR for purposes of phytoremediating heavy metals. We address the agronomic practices that can be used to maximize the remediation potential of plants. Plant roots have limited ability ability mental from soil, mainly because metals have low solubility in the soil solution. The phytoavailability of metal is closely tired to the soil properties and the metabolites that are released by PGPR (e.g., siderophores, organ acids, and plant growth regulators). The role played by PGPR may be accomplished by their direct effect on plant growth dynamics, or indirectly by acidification, chelation, precipitation, or immobilization of heavy metals in the rhizosphere. From performing this review we have formed the following conclusions: The most critical factor is determining how efficient phytoremediation of metal-contaminated soil will be is the rate of uptake of the metal by plants. In turn, this depends on the rate of bioavailability. We know from our review that beneficial bacteria exist tha can alter metal bioavailability of plants. Using these beneficial bacteria improves the performance of phytoremediation of the metal-contaminated sites. Contaminated sites are often nutrient poor. Such soil can be nutrient enriched by applying metal-tolerant microbes that provide key needed plant nutrients. Applying metal-tolerant microbes therefore may be vital in enhancing the detoxification of heavy-metal-contaminated soils (Glick 2003). Plant stress generated by metal-contaminated soils can be countered by enhancing plant defense responses. Responses can be enhanced by alleviating the stress-mediated impact on plants by enzymatic hydrolysis of ACC, which is intermediate in the biosynthetic pathway of ethylene. These plant-microbe partnerships can act as decontaminators by improving phytoremediation. Soil microorganisms play a central role in maintaining soil structure, fertility and in remediating contaminated soils

  2. Investigating the formation and toxicity of nitrogen transformation products of diclofenac and sulfamethoxazole in wastewater treatment plants

    Energy Technology Data Exchange (ETDEWEB)

    Osorio, Victoria; Sanchís, Josep [Water and Soil Quality Research Group, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona (Spain); Abad, Jose Luís [RUBAM-IIQAC-CSIC, Jordi Girona 18-26, Barcelona (Spain); Ginebreda, Antoni; Farré, Marinella [Water and Soil Quality Research Group, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona (Spain); Pérez, Sandra, E-mail: spsqam@idaea.csic.es [Water and Soil Quality Research Group, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona (Spain); Barceló, Damià [Water and Soil Quality Research Group, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona (Spain); Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, Emili Grahit 101, Girona (Spain)

    2016-05-15

    Highlights: • Environmental monitoring of recently discovered TPs of diclofenac in wastewater and surface water. • Quantitative determination of non-detected TPs of sulfamethoxazole in wastewater and impacted surface waters. • Toxicity assessment of diclofenac and its TPs in a panel of standard assays of aquatic organism. • Only nitro-diclofenac proved to be more toxic than the parent compound. - Abstract: Diclofenac (DCF) and sulfamethoxazole (SMX) are highly consumed pharmaceuticals and concentrated in effluents from conventional wastewater treatment plants (WWTPs) since they are not completely eliminated. Under microbial mediated nitrification/denitrification processes occurring in nitrifying activated sludge DCF biotransformed into its nitroso and nitro derivatives (NO-DCF and NO{sub 2}-DCF, respectively). SMX was biotransformed under denitrification conditions in water/sediment batch reactors into its nitro and desamino derivatives (NO{sub 2}-SMX and Des-SMX, respectively). Four transformation products (TPs) from DCF and SMX were analized in wastewaters (WW) and receiving surface waters (SW). Nitrifying/denitrifying-derivatives of DCF and SMX were detected for the first time in WW and SW at one order of magnitude lower than their parent compounds. Relationships observed among levels of NO-DCF, NO{sub 2}-DCF and nitrogen-species tentatively suggested that nitrification/denitrification processes are involved in nitration and nitrosation of DCF during biological WW treatment. Acute toxicity of analytes to Daphnia magna and Vibrio fischeri was assessed individually and in mixtures with other relevant micropollutants. Individual effects showed these compounds as not harmful and not toxic. However, synergism effects observed in mixtures evidenced that contribution of these compounds to overall toxicity of complex environmental samples, should not be dismissed.

  3. Advanced methodology to determine plant stresses using in-situ spectral data

    Science.gov (United States)

    Polinova, Maria; Brook, Anna; Housh, Mashor

    2017-04-01

    Fluorescence method in remote sensing has long been a traditional method estimating plant state. Vegetation indices (VIs) are tool for assessment plants' state based on its spectral characteristics. During the last half-century, in this domain were developed many vegetation indices and even more modifications of these indices. Nowadays, visible range across electromagnetic waves allows assessing plants' health and calculating its physical parameters. One of the VI's capabilities is detecting stress in plants. This approach has application in different areas. For discerning external environment (unnatural) stress from features of plant's development most of VIs have border values for greenness and health. This is the reason for these methods to be superficial and insufficient detecting and estimating stresses on the early stages. This limits plays especial importance in agriculture. Late stress detection leads to irreversible damage in crops and yield loss. We propose new principle of VI analysis for determination unnatural stress on early stages. Novelty of this method is common consideration several VIs related to plant's pigmentation: chlorophyll, carotenoids and anthocyanins. We have tasted this method on two agriculture fields: tomatoes and cotton. The goal of study was to determinate water crop stress at its beginning. A single VI shows reactions on emergence growth stage, fruit producing and ripening phase. It was hard to isolate crops' reaction on water from reaction on growth changes. Nevertheless, we have noted that there is correlation between chlorophyll VIs and carotenoid VIs. The correlation strength was depended on stress type. Based on common VIs analysis we were able to identify dryness and over irrigation stress. In addition, we have determine reaction on fertilizers input. Common VIs analysis can improve existing fluorescence method of remote sensing monitoring. It can find application in areas where the early plant's stress detection is very

  4. Advanced Power Ultra-Uprates of Existing Plants (APPU) Final Scientific/Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Rubiolo, Pablo R. [Westinghouse Electric Company LLC, Pittsburgh, PA (United States). Science and Technology Dept.; Conway, Lawarence E. [Westinghouse Electric Company LLC, Pittsburgh, PA (United States). Science and Technology Dept.; Oriani, Luca [Westinghouse Electric Company LLC, Pittsburgh, PA (United States). Science and Technology Dept.; Lahoda, Edward J. [Westinghouse Electric Company LLC, Pittsburgh, PA (United States). Science and Technology Dept.; DeSilva, Greg [Westinghouse Electric Company LLC, Pittsburgh, PA (United States). Science and Technology Dept.; Hu, Min H. [Westinghouse Electric Company LLC, Pittsburgh, PA (United States). Nuclear Services Division; Hartz, Josh [Westinghouse Electric Company LLC, Pittsburgh, PA (United States). Nuclear Services Division; Bachrach, Uriel [Westinghouse Electric Company LLC, Pittsburgh, PA (United States). Nuclear Services Division; Smith, Larry [Westinghouse Electric Company LLC, Pittsburgh, PA (United States). Nuclear Services Division; Dudek, Daniel F. [Westinghouse Electric Company LLC, Pittsburgh, PA (United States). Nuclear Services Division; Toman, Gary J. [Electric Power Research Inst. (EPRI), Palo Alto, CA (United States); Feng, Dandong [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Hejzlar, Pavel [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Kazimi, Mujid S. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

    2006-03-31

    This project assessed the feasibility of a Power Ultra-Uprate on an existing nuclear plant. The study determined the technical and design limitations of the current components, both inside and outside the containment. Based on the identified plant bottlenecks, the design changes for major pieces of equipment required to meet the Power Ultra-Uprate throughput were determined. Costs for modified pieces of equipment and for change-out and disposal of the replaced equipment were evaluated. These costs were then used to develop capital, fuel and operating and maintenance cost estimates for the Power Ultra-Uprate plant. The cost evaluation indicates that the largest cost components are the replacement of power (during the outage required for the uprate) and the new fuel loading. Based on these results, the study concluded that, for a standard 4-loop plant, the proposed Power Ultra-Uprate is technically feasible. However, the power uprate is likely to be more expensive than the cost (per Kw electric installed) of a new plant when large capacity uprates are considered (>25%). Nevertheless, the concept of the Power Ultra-Uprate may be an attractive option for specific nuclear power plants where a large margin exists in the steam and power conversion system or where medium power increases (~600 MWe) are needed. The results of the study suggest that development efforts on fuel technologies for current nuclear power plants should be oriented towards improving the fuel performance (fretting-wear, corrosion, uranium load, manufacturing, safety) required to achieve higher burnup rather focusing on potential increases in the fuel thermal output.

  5. The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B

    Science.gov (United States)

    Joung, Young Hee; Park, Se Hee; Moon, Ki-Beom; Jeon, Jae-Heung; Cho, Hye-Sun; Kim, Hyun-Soon

    2016-01-01

    Disease prevention through vaccination is considered to be the greatest contribution to public health over the past century. Every year more than 100 million children are vaccinated with the standard World Health Organization (WHO)-recommended vaccines including hepatitis B (HepB). HepB is the most serious type of liver infection caused by the hepatitis B virus (HBV), however, it can be prevented by currently available recombinant vaccine, which has an excellent record of safety and effectiveness. To date, recombinant vaccines are produced in many systems of bacteria, yeast, insect, and mammalian and plant cells. Among these platforms, the use of plant cells has received considerable attention in terms of intrinsic safety, scalability, and appropriate modification of target proteins. Research groups worldwide have attempted to develop more efficacious plant-derived vaccines for over 30 diseases, most frequently HepB and influenza. More inspiring, approximately 12 plant-made antigens have already been tested in clinical trials, with successful outcomes. In this study, the latest information from the last 10 years on plant-derived antigens, especially hepatitis B surface antigen, approaches are reviewed and breakthroughs regarding the weak points are also discussed. PMID:27754367

  6. The CRISPR-Cas system for plant genome editing: advances and opportunities.

    Science.gov (United States)

    Kumar, Vinay; Jain, Mukesh

    2015-01-01

    Genome editing is an approach in which a specific target DNA sequence of the genome is altered by adding, removing, or replacing DNA bases. Artificially engineered hybrid enzymes, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) system are being used for genome editing in various organisms including plants. The CRISPR-Cas system has been developed most recently and seems to be more efficient and less time-consuming compared with ZFNs or TALENs. This system employs an RNA-guided nuclease, Cas9, to induce double-strand breaks. The Cas9-mediated breaks are repaired by cellular DNA repair mechanisms and mediate gene/genome modifications. Here, we provide a detailed overview of the CRISPR-Cas system and its adoption in different organisms, especially plants, for various applications. Important considerations and future opportunities for deployment of the CRISPR-Cas system in plants for numerous applications are also discussed. Recent investigations have revealed the implications of the CRISPR-Cas system as a promising tool for targeted genetic modifications in plants. This technology is likely to be more commonly adopted in plant functional genomics studies and crop improvement in the near future.

  7. The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B

    Directory of Open Access Journals (Sweden)

    Young Hee Joung

    2016-10-01

    Full Text Available Disease prevention through vaccination is considered to be the greatest contribution to public health over the past century. Every year more than 100 million children are vaccinated with the standard World Health Organization (WHO-recommended vaccines including hepatitis B (HepB. HepB is the most serious type of liver infection caused by the hepatitis B virus (HBV, however, it can be prevented by currently available recombinant vaccine, which has an excellent record of safety and effectiveness. To date, recombinant vaccines are produced in many systems of bacteria, yeast, insect, and mammalian and plant cells. Among these platforms, the use of plant cells has received considerable attention in terms of intrinsic safety, scalability, and appropriate modification of target proteins. Research groups worldwide have attempted to develop more efficacious plant-derived vaccines for over 30 diseases, most frequently HepB and influenza. More inspiring, approximately 12 plant-made antigens have already been tested in clinical trials, with successful outcomes. In this study, the latest information from the last 10 years on plant-derived antigens, especially hepatitis B surface antigen, approaches are reviewed and breakthroughs regarding the weak points are also discussed.

  8. Transformation of a wave energy spectrum from encounter to absolute domain when observing from an advancing ship

    DEFF Research Database (Denmark)

    Nielsen, Ulrik Dam

    2017-01-01

    The article presents a practical approach to transform a wave energy spectrum from encounter domain to absolute domain. This problem has its specific relevance, when shipboard sea state estimation is conducted by the buoy analogy; notably for some particular implementation solving for the sea state......” the waves (beam to head sea) but in following sea conditions there exists no unique solution to the problem. Instead, a reasonable approach valid for practical engineering must be applied, and the article outlines one viable solution that can be used to transform a wave spectrum from encounter to absolute...... domain. Specifically, two pseudo algorithms are presented, and good performance is achieved with both algorithms when they are tested at different operational scenarios....

  9. Support vector machine based decision for mechanical fault condition monitoring in induction motor using an advanced Hilbert-Park transform.

    Science.gov (United States)

    Ben Salem, Samira; Bacha, Khmais; Chaari, Abdelkader

    2012-09-01

    In this work we suggest an original fault signature based on an improved combination of Hilbert and Park transforms. Starting from this combination we can create two fault signatures: Hilbert modulus current space vector (HMCSV) and Hilbert phase current space vector (HPCSV). These two fault signatures are subsequently analysed using the classical fast Fourier transform (FFT). The effects of mechanical faults on the HMCSV and HPCSV spectrums are described, and the related frequencies are determined. The magnitudes of spectral components, relative to the studied faults (air-gap eccentricity and outer raceway ball bearing defect), are extracted in order to develop the input vector necessary for learning and testing the support vector machine with an aim of classifying automatically the various states of the induction motor.

  10. The Aggregate Exposure Pathway (AEP): A conceptual framework for advancing exposure science research and transforming risk assessment

    Science.gov (United States)

    Recent advances in analytical methods, biomarker discovery, cell-based assay development, computational tools, sensor/monitor, and omics technology have enabled new streams of exposure and toxicity data to be generated at higher volumes and speed. These new data offer the opport...

  11. Recent advances in understanding the control of secretory proteins by the unfolded protein response in plants.

    Science.gov (United States)

    Hayashi, Shimpei; Wakasa, Yuhya; Takaiwa, Fumio

    2013-04-29

    The membrane transport system is built on the proper functioning of the endoplasmic reticulum (ER). The accumulation of unfolded proteins in the ER lumen (ER stress) disrupts ER homeostasis and disturbs the transport system. In response to ER stress, eukaryotic cells activate intracellular signaling (named the unfolded protein response, UPR), which contributes to the quality control of secretory proteins. On the other hand, the deleterious effects of UPR on plant health and growth characteristics have frequently been overlooked, due to limited information on this mechanism. However, recent studies have shed light on the molecular mechanism of plant UPR, and a number of its unique characteristics have been elucidated. This study briefly reviews the progress of understanding what is happening in plants under ER stress conditions.

  12. [Advances in humans and animals opportunistic pathogens from environment infecting plants by crossing kingdoms].

    Science.gov (United States)

    Huang, Min; Wu, Yixin; He, Pengfei

    2016-02-04

    Some pathogenic microorganisms ubiquitous in the environment could cross kingdoms to infect diverse hosts. Several cross-kingdom human pathogens were summarized in this paper, including Serratia marcescens, Enterobacter cloacae and Pseudomonas aeuriginosa. They are ubiquitous in the nature and could cause plant diseases using the same or different infection strategies with which they infect humans and broaden host range. Among these bacteria, Klebsiella pneumoniae causes top rot disease of maize in the nature, revealing some plants in the environment could serve as a reservoir of various pathogens which might infect animals and probably humans when conditions are favorable, and even potentially harm food. Research on these cross-kingdom pathogens may play a very important role in the epidemiology of human, animal and plant diseases and be a hot topic in environment science.

  13. Advances and Developing Tendency of Water Use Efficiency in Plant Biology

    Institute of Scientific and Technical Information of China (English)

    CHEN Zhao-bo; TANG Jiao-wen; ZHANG Fu

    2009-01-01

    Biological water saving is one of the major fields of water saving agriculture in the future and has an enormous potential in agricultural production. In this paper, the necessity and urgency of developing high water use efficiency in plant biology were dissertated firstly, and the research progresses at home and abroad were reviewed as following aspects: mechanisms of drought resistance and high water use efficiency, criterions for identifying and evaluating drought resistance and water use efficiency, genetic improvement for drought resistance and water use efficiency, water saving irrigation technology based on the physiological regulation and control in crop plants. Major problems in the research field at present were put forward, and development tendency of water use efficiency in plant biology in the future were also discussed.

  14. Constitutive expression of the tzs gene from Agrobacterium tumefaciens virG mutant strains is responsible for improved transgenic plant regeneration in cotton meristem transformation.

    Science.gov (United States)

    Ye, Xudong; Chen, Yurong; Wan, Yuechun; Hong, Yun-Jeong; Ruebelt, Martin C; Gilbertson, Larry A

    2016-03-01

    KEY MESSAGE : virG mutant strains of a nopaline type of Agrobacterium tumefaciens increase the transformation frequency in cotton meristem transformation. Constitutive cytokinin expression from the tzs gene in the virG mutant strains is responsible for the improvement. Strains of Agrobacterium tumefaciens were tested for their ability to improve cotton meristem transformation frequency. Two disarmed A. tumefaciens nopaline strains with either a virGN54D constitutively active mutation or virGI77V hypersensitive induction mutation significantly increased the transformation frequency in a cotton meristem transformation system. The virG mutant strains resulted in greener explants after three days of co-culture in the presence of light, which could be attributed to a cytokinin effect of the mutants. A tzs knockout strain of virGI77V mutant showed more elongated, less green explants and decreased cotton transformation frequency, as compared to a wild type parental strain, suggesting that expression of the tzs gene is required for transformation frequency improvement in cotton meristem transformation. In vitro cytokinin levels in culture media were tenfold higher in the virGN54D strain, and approximately 30-fold higher in the virGI77V strain, in the absence of acetosyringone induction, compared to the wild type strain. The cytokinin level in the virGN54D strain is further increased upon acetosyringone induction, while the cytokinin level in the virGI77V mutant is decreased by induction, suggesting that different tzs gene expression regulation mechanisms are present in the two virG mutant strains. Based on these data, we suggest that the increased cytokinin levels play a major role in increasing Agrobacterium attachment and stimulating localized division of the attached plant cells.

  15. Recent advances in plant sterol%植物甾醇的研究进展

    Institute of Scientific and Technical Information of China (English)

    张斌; 郁听; 栗磊; 孟祥河

    2015-01-01

    研究发现,植物甾醇能同时降低血清中总胆固醇含量和低密度脂蛋白胆固醇水平,并在2010年被我国批准为新资源食品.文中简要介绍了植物甾醇的性质、来源及生理功能,总结了典型的植物甾醇分离提取工艺,并综述了植物甾醇的改性研究和甾醇氧化产物研究进展,重点阐述了天然植物甾醇及其氧化物的安全性问题,为甾醇的工业应用提供一定的借鉴作用.%Researches have found out that plant sterol could reduce total cholesterol and lower the density lipoprotein cholesterol in serum.And it had been approved as a new resource food by our country in 2010.This paper briefly introduced the properties,sources and physiological functions of plant sterol.At the same time,we summarized the processes of separation and extraction of typical plant sterol,as well as the modification research and progress of research of plant sterol oxidation products.We put emphasis on the safety of natural plant sterol and its oxidation products,which could provide a certain reference for industrial application of plant sterol.

  16. Techno-economic projections for advanced small solar thermal electric power plants to years 1990--2000

    Energy Technology Data Exchange (ETDEWEB)

    Fujita, T.; Manvi, R.; Roschke, E.J.; El Gabalawi, N.; Herrera, G.; Kuo, T.J.; Chen, K.H.

    1978-11-15

    Advanced technologies applicable to solar thermal electric power systems in the 1990--2000 time-frame are delineated for power applications that fulfill a wide spectrum of small power needs with primary emphasis on power ratings <10 MWe. Techno-economic projections of power system characteristics (energy and capital costs as a function of capacity factor) are made based on development of identified promising technologies. The key characteristic of advanced technology systems is an efficient low-cost solar energy collection while achieving high temperatures for efficient energy conversion. Two-axis tracking systems such as the central receiver or power tower concept and distributed parabolic dish receivers possess this characteristic. For these two basic concepts, advanced technologies including, e.g., conversion systems such as Stirling engines, Brayton/Rankine combined cycles and storage/transport concepts encompassing liquid metals, and reversible-reaction chemical systems are considered. In addition to techno-economic aspects, technologies are also judged in terms of factors such as developmental risk, relative reliability, and probability of success. Improvements accruing to projected advanced technology systems are measured with respect to current (or pre-1985) steam-Rankine systems, as represented by the central receiver pilot plant being constructed near Barstow, California. These improvements, for both central receivers and parabolic dish systems, indicate that pursuit of advanced technology across a broad front can result in post-1985 solar thermal systems having the potential of approaching the goal of competitiveness with conventional power systems; i.e., capital costs of $600 kWe and energy costs of 50 mills/kWe-hr (1977 dollars).

  17. The advance of transgenic plants%转基因植物的现状

    Institute of Scientific and Technical Information of China (English)

    王亮; 游松

    2001-01-01

    近20多年以来,随着分子生物学的不断发展,植物基因工程取得了很多突破,本文介绍了转基因植物在生物药品、食品、工业用酶和农业四方面的发展现状。%In the past 20 years, dramatic changes have taken place in the field of plant biotechnology with the development of molecular biotechnology. This paper reviewed the applications of transgenic plants in pharmaceutics, food, industrial enzymes and agriculture.

  18. Advancing our understanding of plant adaptation to metal polluted environments - new insights from Biscutella laevigata

    Science.gov (United States)

    Babst-Kostecka, Alicja; Waldmann, Patrik; Frérot, Hélène; Vollenweider, Pierre

    2016-04-01

    The legacy of industrial pollution alters ecosystems, particularly at post-mining sites where metal trace elements have created toxic conditions that trigger rapid plant adaptation. Apart from the purely scientific merits, in-depth knowledge of the mechanisms underlying plant adaptation to metal contamination is beneficial for the economic and societal sectors because of its application in bioengineering (e.g. phytoremediation or biofortification). An important process is the evolution and/or enhancement of metal tolerance, a trait that has predominantly been studied by applying acute metal stress on species that allocate large quantities of certain metals to their foliage (so-called hyperaccumulators). As the vast majority of vascular plants does not hyperaccumulate metals, more efforts are needed to investigate non-hyperaccumulating species and thereby broaden understanding of biological mechanisms underlying metal tolerance. The pseudometallophyte Biscutella laevigata has shown potential in this respect, but its characteristics are insufficiently understood. We determined the zinc tolerance level and various plant responses to environmentally relevant zinc concentrations in ten metallicolous and non-metallicolous B. laevigata populations. In a two-phase hydroponic experiment, we scored multiple morphological and physiological traits (e.g. biomass, visible stress symptoms, element content in foliage) and assessed phenotypic variability within plant families. The structure of these quantitative traits was compared to that of neutral molecular markers to test, whether natural selection caused population differentiation in zinc tolerance. While all genotypes were tolerant compared to a zinc sensitive reference species, we found congruent trends toward higher tolerance in metallicolous compared to non-metallicolous plants. We identified the most indicative parameters for these differences and find that enhanced zinc tolerance in metallicolous populations is driven by

  19. Polyacetylenes from terrestrial plants and fungi: Recent phytochemical and biological advances.

    Science.gov (United States)

    Negri, Roberto

    2015-10-01

    Polyacetylenes are a class of polyketides related to fatty acids that occur in plants, fungi, marine organisms and animals. These compounds show a pleiotropic profile of bioactivity, that includes antitumor, antibacterial, antimicrobial or antifungal properties. Because of this, the literature on these compounds has grown exponentially, and this review aims at summarizing the inventory of polyacetylenes occurring in terrestrial eukaryotic organisms (plants and fungi) during the last 15 years, and at discussing progress in their bioactivities and in the identification of their biological targets.

  20. [Advances in influence of UV-B radiation on medicinal plant secondary metabolism].

    Science.gov (United States)

    Wu, Yang; Fang, Minfeng; Yue, Ming; Chai, Yongfu; Wang, Hui; Li, Yifei

    2012-08-01

    Stratospheric ozone depletion results in an increased level of solar UV-B radiation (UV-B, 280-320 nm) reaching the earth surface. By the effect of UV-B radiation, various medicinal active ingredients changed because of the change of gene expression, enzyme activity and secondary metabolism, clinical effect is also changed. The research status of UV-B radiation and the accumulation of plant secondary metabolites in the past 10 years were summarized in this paper to supply reference for cultivation and exploitation of the medicinal plants.

  1. Ecohydrological advances and applications in plant-water relations research: a review

    NARCIS (Netherlands)

    Asbjornsen, H.; Goldsmith, G.R.; Alvarado-Barrientos, M.S.; Rebel, K.T.; Osch, F. van; Rietkerk, M.G.; Chen, J.; Gotsch, S.; Tobon, C.; Geissert, D.R.; Gomez-Tagle, A.; Vache, K.; Dawson, T.E.

    2011-01-01

    Aims The field of ecohydrology is providing new theoretical frameworks and methodological approaches for understanding the complex interactions and feedbacks between vegetation and hydrologic flows at multiple scales. Here we review some of the major scientific and technological advances in ecohydro

  2. Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid.

    Science.gov (United States)

    Zyprian, E; Kado, C I

    1990-08-01

    A new binary vector system for Agrobacterium-mediated plant transformation was developed. A set of four mini-T vectors comprised of T-DNA border sequences from nopaline-type Ti-plasmid pTiC58 flanking a chimaeric hygromycin-resistance gene for selection of transformants and up to eight unique restriction sites for cloning foreign DNA was constructed on a broad-host replicon containing the oriV of plasmid pSa. In two of the constructs these multiple cloning sites are flanked by a strong promoter to activate transcription of inserted DNA in planta. High-efficiency transformation was prompted by a high-copy, stable virulence helper plasmid pUCD2614, which contains a cloned virulence region of pTiC58 and tandem copies of the par locus of plasmid pTAR. Southern blot hybridization and genetic analyses of the progeny of transformed plants showed that the hygromycin resistance gene was stably inherited.

  3. Effect of water withdrawal on formation of free radical, proline accumulation and activities of antioxidant enzymes in ZAT12-transformed transgenic tomato plants.

    Science.gov (United States)

    Chandra Rai, Avinash; Singh, Major; Shah, Kavita

    2012-12-01

    Water stress often leads to the accumulation of reactive oxygen species (ROS) and their excessive production alters the activities of enzymes involved in their removal. ZAT12 is a member of stress-responsive C(2)H(2) type Zinc Finger Protein (ZFP) reported to control the expression of several stress-activated genes in plants through ROS signaling. The ZAT12-transformed tomato lines (cv. H-86 variety Kashi Vishesh) when subjected to water withdrawal for 7, 14 and 21 days revealed significant and consistent changes in activities of enzymes SOD, CAT, APX, GR and POD paralleled with an increased proline levels. Unlike that in wild-type tomato, the leaf superoxide anion and hydrogen peroxide concentrations in the transformed tomato plants did not alter much, suggesting a well regulated formation of free radicals suppressing oxidative stress in the latter. Results suggest BcZAT12-transformed tomato lines ZT1, ZT2 and ZT6 to be better adapted to drought stress tolerance by accumulation of osmolyte proline and increased antioxidant response triggered by the ZAT12 gene. Therefore, the ZAT12-transformed tomato cv. H-86 lines will prove useful for higher yield of tomato crop in regions affected with severe drought stress.

  4. Research progress in microbial transformation of plant secondary metabolite%微生物转化植物次生代谢产物研究进展

    Institute of Scientific and Technical Information of China (English)

    邹宇; 马堃; 尹冬梅

    2013-01-01

    植物次生代谢产物具有许多重要的生理功能,它的微生物转化成为近年来的研究热点.本文综述了植物次生代谢产物的种类和功能以及微生物转化植物次生代谢产物的类型和特点,展望了微生物转化技术在生物活性物质生产和医药保健品研发等领域的广阔应用前景.%The plant secondary metabolites has many important physiological functions and its microbial transformation has become a hot research topic in this field in recent years.In this paper,several species and function of plant secondary metabolite had been summarized.Meanwhile,various types and characteristics of microbial transformation of plant secondary metabolite had been introduced.Moreover,it was predicted that microbial transformation would have wide application prospect in production of bioactive substances and development of pharmaceuticals and health foods.

  5. Performance analysis and optimization of an advanced pharmaceutical wastewater treatment plant through a visual basic software tool (PWWT.VB).

    Science.gov (United States)

    Pal, Parimal; Thakura, Ritwik; Chakrabortty, Sankha

    2016-05-01

    A user-friendly, menu-driven simulation software tool has been developed for the first time to optimize and analyze the system performance of an advanced continuous membrane-integrated pharmaceutical wastewater treatment plant. The software allows pre-analysis and manipulation of input data which helps in optimization and shows the software performance visually on a graphical platform. Moreover, the software helps the user to "visualize" the effects of the operating parameters through its model-predicted output profiles. The software is based on a dynamic mathematical model, developed for a systematically integrated forward osmosis-nanofiltration process for removal of toxic organic compounds from pharmaceutical wastewater. The model-predicted values have been observed to corroborate well with the extensive experimental investigations which were found to be consistent under varying operating conditions like operating pressure, operating flow rate, and draw solute concentration. Low values of the relative error (RE = 0.09) and high values of Willmott-d-index (d will = 0.981) reflected a high degree of accuracy and reliability of the software. This software is likely to be a very efficient tool for system design or simulation of an advanced membrane-integrated treatment plant for hazardous wastewater.

  6. Research advances in transgenic plant vaccines%转基因植物疫苗的研究进展

    Institute of Scientific and Technical Information of China (English)

    肖明珠; 朱家勇

    2010-01-01

    In recent years, with the development of immunology, genetics and molecular biology, the vaccination programs are taking on a prosperous evolvement. In particular, the technology of the use of transgenic plants to produce human or animal vaccines receives increasing attention. Expressing vaccine antigens in transgenic plants opens up a new avenue for producing oral/edible vaccines. Up to now, many transgenic plant vaccines have been investigated and developed. In this review, recent research advances and basic action mechanisms of transgenic plant vaccines and the issues of high concern, such as the influence on environment and biosafety, are discussed. Special attention is given to the prospects and limitations on transgenic plant vaccines.%近年来,随着免疫学、遗传学和分子生物学的发展,疫苗研究得到了迅速发展,尤其是利用转基因植物技术生产植物疫苗的研究受到了广泛关注.通过在转基因植物的可食用部位表达抗原来生产人或动物疫苗的技术为可食性疫苗的研制开辟了新途径.目前已有很多转基因植物疫苗投入研究和开发.此文综述了近几年转基因植物疫苗的研究现状、作用机制及安全性评价等,并在对转基因植物疫苗存在的问题进行分析的基础上,对其研究前景提出了展望.

  7. Advances in seed conservation of wild plant species: a review of recent research.

    Science.gov (United States)

    Hay, Fiona R; Probert, Robin J

    2013-01-01

    Seed banking is now widely used for the ex situ conservation of wild plant species. Many seed banks that conserve wild species broadly follow international genebank guidelines for seed collection, processing, storage, and management. However, over the last 10-20 years, problems and knowledge gaps have been identified, which have led to more focused seed conservation research on diverse species. For example, there is now greater ecogeographic understanding of seed storage behaviour and of the relative longevity of orthodox seeds, and we are therefore able to predict which species should be conserved using cryostorage techniques; seed development studies have identified when seeds should be harvested for maximal tolerance of desiccation and longevity in storage, as well as highlighting how seed development can vary between species; and there is now a wealth of literature on the dormancy-breaking and germination requirements of wild species which, as well as enabling better management of accessions, will also mean that their use in restoration, species reintroduction, or for evaluation for other applications is possible. Future research may be focused, for example, on nursery and plant production systems for wild plant species that maximize genetic diversity, so that introduced seeds and plant materials have the resilience to cope with future environmental stresses.

  8. Advanced Multi-Product Coal Utilization By-Product Processing Plant

    Energy Technology Data Exchange (ETDEWEB)

    John Groppo; Thomas Robl

    2006-09-30

    The objective of the project is to build a multi-product ash beneficiation plant at Kentucky Utilities 2,200-MW Ghent Generating Station, located in Carroll County, Kentucky. This part of the study includes an investigation of the secondary classification characteristics of the ash feedstock excavated from the lower ash pond at Ghent Station.

  9. Advances in biocontrol mechanism and application of Trichoderma spp. for plant diseases

    Institute of Scientific and Technical Information of China (English)

    HUANG Caihong; YANG Qian

    2007-01-01

    Trichoderma spp. is a filamentous soil fungus known as an effective biocontrol agent of a range of important airborne and soilborne pathogens, it has universal distribution and economic importance. This article reviewed the researches on biocontrol mechanism for plant diseases and application of Trichoderma spp., especially Trichoderma harzianum in recent years.

  10. Manipulating photorespiration to increase plant productivity. Recent advances and perspectives for crop improvement

    Science.gov (United States)

    Recycling of the 2-phosphoglycolate generated by the oxygenase reaction of Rubisco requires a complex and energy-consuming set of reactions collectively known as the photorespiratory cycle. Several approaches have been proposed with the aim of producing plants with reduced rates of photorespiratory ...

  11. Development of Advanced Technologies to Reduce Design, Fabrication and Construction Costs for Future Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    DiNunzio, Camillo A. [Framatome ANP DE& S, Marlborough, MA (United States); Gupta, Abhinav [Univ. of North Carolina, Raleigh, NC (United States); Golay, Michael [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Luk, Vincent [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Turk, Rich [Westinghouse Electric Company Nuclear Systems, Windsor, CT (United States); Morrow, Charles [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Jin, Geum-Taek [Korea Power Engineering Company Inc., Yongin-si, Gyeonggi-do (Korea, Republic of)

    2002-11-30

    This report presents a summation of the third and final year of a three-year investigation into methods and technologies for substantially reducing the capital costs and total schedule for future nuclear plants. In addition, this is the final technical report for the three-year period of studies.

  12. A simulation benchmark to evaluate the performance of advanced control techniques in biological wastewater treatment plants

    Directory of Open Access Journals (Sweden)

    Sotomayor O.A.Z.

    2001-01-01

    Full Text Available Wastewater treatment plants (WWTP are complex systems that incorporate a large number of biological, physicochemical and biochemical processes. They are large and nonlinear systems subject to great disturbances in incoming loads. The primary goal of a WWTP is to reduce pollutants and the second goal is disturbance rejection, in order to obtain good effluent quality. Modeling and computer simulations are key tools in the achievement of these two goals. They are essential to describe, predict and control the complicated interactions of the processes. Numerous control techniques (algorithms and control strategies (structures have been suggested to regulate WWTP; however, it is difficult to make a discerning performance evaluation due to the nonuniformity of the simulated plants used. The main objective of this paper is to present a benchmark of an entire biological wastewater treatment plant in order to evaluate, through simulations, different control techniques. This benchmark plays the role of an activated sludge process used for removal of organic matter and nitrogen from domestic effluents. The development of this simulator is based on models widely accepted by the international community and is implemented in Matlab/Simulink (The MathWorks, Inc. platform. The benchmark considers plant layout and the effects of influent characteristics. It also includes a test protocol for analyzing the open and closed-loop responses of the plant. Examples of control applications in the benchmark are implemented employing conventional PI controllers. The following common control strategies are tested: dissolved oxygen (DO concentration-based control, respirometry-based control and nitrate concentration-based control.

  13. Effects of advanced treatment systems on the removal of antibiotic resistance genes in wastewater treatment plants from Hangzhou, China.

    Science.gov (United States)

    Chen, Hong; Zhang, Mingmei

    2013-08-06

    This study aimed at quantifying the concentration and removal of antibiotic resistance genes (ARGs) in three municipal wastewater treatment plants (WWTPs) employing different advanced treatment systems [biological aerated filter, constructed wetland, and ultraviolet (UV) disinfection]. The concentrations of tetM, tetO, tetQ, tetW, sulI, sulII, intI1, and 16S rDNA genes were examined in wastewater and biosolid samples. In municipal WWTPs, ARG reductions of 1-3 orders of magnitude were observed, and no difference was found among the three municipal WWTPs with different treatment processes (p > 0.05). In advanced treatment systems, 1-3 orders of magnitude of reductions in ARGs were observed in constructed wetlands, 0.6-1.2 orders of magnitude of reductions in ARGs were observed in the biological aerated filter, but no apparent decrease by UV disinfection was observed. A significant difference was found between constructed wetlands and biological filter (p constructed wetlands and UV disinfection (p constructed wetlands, significant correlations were observed in the removal of ARGs and 16S rDNA genes (R(2) = 0.391-0.866; p Constructed wetlands not only have the comparable ARG removal values with WWTP (p > 0.05) but also have the advantage in ARG relative abundance removal, and it should be given priority to be an advanced treatment system for further ARG attenuation from WWTP.

  14. Occurrence, fate and risk assessment of parabens and their chlorinated derivatives in an advanced wastewater treatment plant.

    Science.gov (United States)

    Li, Wenhui; Shi, Yali; Gao, Lihong; Liu, Jiemin; Cai, Yaqi

    2015-12-30

    In the present study, parabens, p-hydroxybenzoic acid (PHBA) and chlorinated derivatives, were simultaneously determined in wastewater and sludge samples along the whole process in an advanced wastewater treatment plant (WWTP). Nine target compounds were detected in this WWTP, and methylparaben and PHBA were the dominant compounds in these samples. It is noteworthy that octylparaben with longer chain was firstly detected in this work. Mass balance results showed that 91.8% of the initial parabens mass loading was lost mainly due to degradation, while the contribution of sorption and output of primary and excess sludge was much less (7.5%), indicating that biodegradation played a significant role in the removal of parabens during the conventional treatment process. Specifically, parabens were mainly degraded in the anaerobic tank, and PHBA could be effectively removed at high rates after the advanced treatment. However, both biodegradation and adsorption accounted for minor contribution to the removal of chlorinated parabens during conventional treatment process, and they were only scantly removed by conventional treatment (33.9-40.7%) and partially removed by advanced treatment (59.2-82.8%). Risk assessment indicated that parabens and their chlorinated derivatives in second and tertiary effluent are not likely to produce biological effects on aquatic ecosystems. Copyright © 2015 Elsevier B.V. All rights reserved.

  15. In-plant testing of a novel coal cleaning circuit using advanced technologies, Quarterly report, March 1 - May 31, 1996

    Energy Technology Data Exchange (ETDEWEB)

    Honaker, R.Q. [Southern Illinois Univ., Carbondale, IL (United States); Reed, S.; Mohanty, M.K. [Kerr-McGee Coal Corp., Oklahoma City, OK (United States)

    1996-12-31

    Research conducted at Southern Illinois University at Carbondale over the past two years has identified highly efficient methods for treating fine coal (i.e., -28 mesh). In this study, a circuit comprised of the three advanced fine coal cleaning technologies is being tested in an operating preparation plant to evaluate circuit performance and to compare the performance with the current technologies used to treat -16 mesh fine coal. The circuit integrated a Floatex hydrosizer, a Falcon concentrator and a Jameson froth flotation cell. The Floatex hydrosizer is being used as a primary cleaner for the nominally -16 mesh Illinois No. 5 fine coal circuit feed. The overflow of the Floatex is screened at 48 mesh using a Sizetec vibratory screen to produce a clean coal product from the screen overflow. The screen overflow is further treated by the Falcon and Jameson Cell. During this reporting period, tests were initiated on the fine coal circuit installed at the Kerr-McGee Galatia preparation plant. The circuit was found to reduce both the ash content and the pyritic sulfur content. Additional in-plant circuitry tests are ongoing.

  16. Dynamic modeling, simulation and control design of an advanced micro-hydro power plant for distributed generation applications

    Energy Technology Data Exchange (ETDEWEB)

    Marquez, J.L. [Instituto de Energia Electrica, Universidad Nacional de San Juan, Av. Libertador San Martin Oeste 1109, J5400ARL San Juan (Argentina); Molina, M.G. [CONICET, Instituto de Energia Electrica, Universidad Nacional de San Juan, Av. Libertador San Martin Oeste 1109, J5400ARL San Juan (Argentina); Pacas, J.M. [Institut fuer Leistungselektronik und Elektrische Antriebe, Universitaet Siegen, Fachbereich 12 Hoelderlinstr 3, D 57068 Siegen (Germany)

    2010-06-15

    A small-scale hydropower station is usually a run-of-river plant that uses a fixed speed drive with mechanical regulation of the turbine water flow rate for controlling the active power generation. This design enables to reach high efficiency over a wide range of water flows but using a complex operating mechanism, which is in consequence expensive and tend to be more affordable for large systems. This paper proposes an advanced structure of a micro-hydro power plant (MHPP) based on a smaller, lighter, more robust and more efficient higher-speed turbine. The suggested design is much simpler and eliminates all mechanical adjustments through a novel electronic power conditioning system for connection to the electric grid. In this way, it allows obtaining higher reliability and lower cost of the power plant. A full detailed model of the MHPP is derived and a new three-level control scheme is designed. The dynamic performance of the proposed MHPP is validated through digital simulations and employing a small-scale experimental set-up. (author)

  17. ADVANCED MULTI-PRODUCT COAL UTILIZATION BY-PRODUCT PROCESSING PLANT

    Energy Technology Data Exchange (ETDEWEB)

    Robert Jewell; Thomas Robl; John Groppo

    2005-03-01

    The objective of the project is to build a multi-product ash beneficiation plant at Kentucky Utilities 2,200-MW Ghent Generating Station, located in Carroll County, Kentucky. This part of the study includes the examination of the feedstocks for the beneficiation plant. The ash, as produced by the plant, and that stored in the lower pond were examined. The ash produced by the plant was found to be highly variable as the plant consumes high and low sulfur bituminous coal, in Units 1 and 2 and a mixture of subbituminous and bituminous coal in Units 3 and 4. The ash produced reflected this consisting of an iron-rich ({approx}24%, Fe{sub 2}O{sub 3}), aluminum rich ({approx}29% Al{sub 2}O{sub 3}) and high calcium (6%-7%, CaO) ash, respectively. The LOI of the ash typically was in the range of 5.5% to 6.5%, but individual samples ranged from 1% to almost 9%. The lower pond at Ghent is a substantial body, covering more than 100 acres, with a volume that exceeds 200 million cubic feet. The sedimentation, stratigraphy and resource assessment of the in place ash was investigated with vibracoring and three-dimensional, computer-modeling techniques. Thirteen cores to depths reaching nearly 40 feet, were retrieved, logged in the field and transported to the lab for a series of analyses for particle size, loss on ignition, petrography, x-ray diffraction, and x-ray fluorescence. Collected data were processed using ArcViewGIS, Rockware, and Microsoft Excel to create three-dimensional, layered iso-grade maps, as well as stratigraphic columns and profiles, and reserve estimations. The ash in the pond was projected to exceed 7 million tons and contain over 1.5 million tons of coarse carbon, and 1.8 million tons of fine (<10 {micro}m) glassy pozzolanic material. The size, quality and consistency of the ponded material suggests that it is the better feedstock for the beneficiation plant.

  18. An Ecology for Cities: A Transformational Nexus of Design and Ecology to Advance Climate Change Resilience and Urban Sustainability

    Directory of Open Access Journals (Sweden)

    Daniel L. Childers

    2015-03-01

    Full Text Available Cities around the world are facing an ever-increasing variety of challenges that seem to make more sustainable urban futures elusive. Many of these challenges are being driven by, and exacerbated by, increases in urban populations and climate change. Novel solutions are needed today if our cities are to have any hope of more sustainable and resilient futures. Because most of the environmental impacts of any project are manifest at the point of design, we posit that this is where a real difference in urban development can be made. To this end, we present a transformative model that merges urban design and ecology into an inclusive, creative, knowledge-to-action process. This design-ecology nexus—an ecology for cities—will redefine both the process and its products. In this paper we: (1 summarize the relationships among design, infrastructure, and urban development, emphasizing the importance of joining the three to achieve urban climate resilience and enhance sustainability; (2 discuss how urban ecology can move from an ecology of cities to an ecology for cities based on a knowledge-to-action agenda; (3 detail our model for a transformational urban design-ecology nexus, and; (4 demonstrate the efficacy of our model with several case studies.

  19. Advanced Techniques for In-Situ Monitoring of Phase Transformations During Welding Using Synchrotron-Based X-Ray Diffraction

    Energy Technology Data Exchange (ETDEWEB)

    Elmer, J W; Palmer, T A; Zhang, W; DebRoy, T

    2005-06-05

    Understanding the evolution of microstructure in welds is an important goal of welding research because of the strong correlation between weld microstructure and weld properties. To achieve this goal it is important to develop a quantitative measure of phase transformations encountered during welding in order to ultimately develop methods for predicting weld microstructures from the characteristics of the welding process. To aid in this effort, synchrotron radiation methods have been developed at Lawrence Livermore National Laboratory (LLNL) for direct observation of microstructure evolution during welding. Using intense, highly collimated synchrotron radiation, the atomic structure of the weld heat affected and fusion zones can be probed in real time. Two synchrotron-based techniques, known as spatially resolved (SRXRD) and time resolved (TRXRD) x-ray diffraction, have been developed for these investigations. These techniques have now been used to investigate welding induced phase transformations in titanium alloys, low alloy steels, and stainless steel alloys. This paper will provide a brief overview of these methods and will discuss microstructural evolution during the welding of low carbon (AISI 1005) and medium carbon (AISI 1045) steels where the different levels of carbon influence the evolution of microstructures during welding.

  20. CRISPR/Cas9: an advanced tool for editing plant genomes.

    Science.gov (United States)

    Samanta, Milan Kumar; Dey, Avishek; Gayen, Srimonta

    2016-10-01

    To meet current challenges in agriculture, genome editing using sequence-specific nucleases (SSNs) is a powerful tool for basic and applied plant biology research. Here, we describe the principle and application of available genome editing tools, including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeat associated CRISPR/Cas9 system. Among these SSNs, CRISPR/Cas9 is the most recently characterized and rapidly developing genome editing technology, and has been successfully utilized in a wide variety of organisms. This review specifically illustrates the power of CRISPR/Cas9 as a tool for plant genome engineering, and describes the strengths and weaknesses of the CRISPR/Cas9 technology compared to two well-established genome editing tools, ZFNs and TALENs.

  1. Advances in plant cell type-specific genome-wide studies of gene expression

    Institute of Scientific and Technical Information of China (English)

    Ying WANG; Yuling JIAO

    2011-01-01

    Cell is the functional unit of life.To study the complex interactions of systems of biological molecules,it is crucial to dissect these molecules at the cell level.In recent years,major progresses have been made by plant biologists to profile gene expression in specific cell types at the genome-wide level.Approaches based on the isolation of cells,polysomes or nuclei have been developed and successfully used for studying the cell types from distinct organs of several plant species.These cell-level data sets revealed previously unrecognized cellular properties,such as cell-specific gene expression modules and hormone response centers,and should serve as essential resources for functional genomic analyses.Newly developed technologies are more affordable to many laboratories and should help to provide new insights at the cellular resolution in the near future.

  2. Elimination of micropollutants and transformation products from a wastewater treatment plant effluent through pilot scale ozonation followed by various activated carbon and biological filters.

    Science.gov (United States)

    Knopp, Gregor; Prasse, Carsten; Ternes, Thomas A; Cornel, Peter

    2016-09-01

    Conventional wastewater treatment plants are ineffective in removing a broad range of micropollutants, resulting in the release of these compounds into the aquatic environment, including natural drinking water resources. Ozonation is a suitable treatment process for micropollutant removal, although, currently, little is known about the formation, behavior, and removal of transformation products (TP) formed during ozonation. We investigated the elimination of 30 selected micropollutants (pharmaceuticals, X-ray contrast media, industrial chemicals, and TP) by biological treatment coupled with ozonation and, subsequently, in parallel with two biological filters (BF) or granular activated carbon (GAC) filters. The selected micropollutants were removed to very different extents during the conventional biological wastewater treatment process. Ozonation (specific ozone consumption: 0.87 ± 0.29 gO3 gDOC(-1), hydraulic retention time: 17 ± 3 min) eliminated a large number of the investigated micropollutants. Although 11 micropollutants could still be detected after ozonation, most of these were eliminated in subsequent GAC filtration at bed volumes (BV) of approximately 25,000 m(3) m(-3). In contrast, no additional removal of micropollutants was achieved in the BF. Ozonation of the analgesic tramadol led to the formation of tramadol-N-oxide that is effectively eliminated by GAC filters, but not by BF. For the antiviral drug acyclovir, the formation of carboxy-acyclovir was observed during activated sludge treatment, with an average concentration of 3.4 ± 1.4 μg L(-1) detected in effluent samples. Subsequent ozonation resulted in the complete elimination of carboxy-acyclovir and led to the formation of N-(4-carbamoyl-2-imino-5-oxo imidazolidin)-formamido-N-methoxyacetetic acid (COFA; average concentration: 2.6 ± 1.0 μg L(-1)). Neither the BF nor the GAC filters were able to remove COFA. These results highlight the importance of considering TP in the

  3. Systems Analyses of Advanced Brayton Cycles For High Efficiency Zero Emission Plants

    Energy Technology Data Exchange (ETDEWEB)

    A. D. Rao; J. Francuz; H. Liao; A. Verma; G. S. Samuelsen

    2006-11-01

    Table 1 shows that the systems efficiency, coal (HHV) to power, is 35%. Table 2 summarizes the auxiliary power consumption within the plant. Thermoflex was used to simulate the power block and Aspen Plus the balance of plant. The overall block flow diagram is presented in Figure A1.3-1 and the key unit process flow diagrams are shown in subsequent figures. Stream data are given in Table A1.3-1. Equipment function specifications are provided in Tables A1.3-2 through 17. The overall plant scheme consists of a cryogenic air separation unit supplying 95% purity O{sub 2} to GE type high pressure (HP) total quench gasifiers. The raw gas after scrubbing is treated in a sour shift unit to react the CO with H{sub 2}O to form H{sub 2} and CO{sub 2}. The gas is further treated to remove Hg in a sulfided activated carbon bed. The syngas is desulfurized and decarbonized in a Selexol acid gas removal unit and the decarbonized syngas after humidification and preheat is fired in GE 7H type steam cooled gas turbines. Intermediate pressure (IP) N{sub 2} from the ASU is also supplied to the combustors of the gas turbines as additional diluent for NOx control. A portion of the air required by the ASU is extracted from the gas turbines. The plant consists of the following major process units: (1) Air Separation Unit (ASU); (2) Gasification Unit; (3) CO Shift/Low Temperature Gas Cooling (LTGC) Unit; (4) Acid Gas Removal Unit (AGR) Unit; (5) Fuel Gas Humidification Unit; (6) Carbon Dioxide Compression/Dehydration Unit; (7) Claus Sulfur Recovery/Tail Gas Treating Unit (SRU/TGTU); and (8) Power Block.

  4. Recent advances in understanding of meiosis initiation and the apomictic pathway in plants

    OpenAIRE

    Chung-Ju Rachel Wang; Ching-Chih eTseng

    2014-01-01

    Meiosis, a specialized cell division to produce haploid cells, marks the transition from a sporophytic to a gametophytic generation in the life cycle of plants. In angiosperms, meiosis takes place in sporogenous cells that develop de novo from somatic cells in anthers or ovules. A successful transition from the mitotic cycle to the meiotic program in sporogenous cells is crucial for sexual reproduction. By contrast, when meiosis is bypassed or a mitosis-like division occurs to produce unreduc...

  5. Recent advances in the analysis of metal hyperaccumulation and hypertolerance in plants using proteomics

    OpenAIRE

    Giovanni eDalCorso; Elisa eFasani; Antonella eFurini

    2013-01-01

    Hyperaccumulator/hypertolerant plant species have evolved strategies allowing them to grow in metal-contaminated soils, where they accumulate high concentrations of heavy metals in their shoots without signs of toxicity. The mechanisms that allow enhanced metal uptake, root-to-shoot translocation and detoxification in these species are not fully understood. Complementary approaches such as transcriptomic-based DNA microarrays and proteomics have recently been used to gain insight into the mol...

  6. A simulation benchmark to evaluate the performance of advanced control techniques in biological wastewater treatment plants

    OpenAIRE

    Sotomayor O.A.Z.; Park S.W.; Garcia C

    2001-01-01

    Wastewater treatment plants (WWTP) are complex systems that incorporate a large number of biological, physicochemical and biochemical processes. They are large and nonlinear systems subject to great disturbances in incoming loads. The primary goal of a WWTP is to reduce pollutants and the second goal is disturbance rejection, in order to obtain good effluent quality. Modeling and computer simulations are key tools in the achievement of these two goals. They are essential to describe, predict ...

  7. Empirical scoring functions for advanced protein-ligand docking with PLANTS.

    Science.gov (United States)

    Korb, Oliver; Stützle, Thomas; Exner, Thomas E

    2009-01-01

    In this paper we present two empirical scoring functions, PLANTS(CHEMPLP) and PLANTS(PLP), designed for our docking algorithm PLANTS (Protein-Ligand ANT System), which is based on ant colony optimization (ACO). They are related, regarding their functional form, to parts of already published scoring functions and force fields. The parametrization procedure described here was able to identify several parameter settings showing an excellent performance for the task of pose prediction on two test sets comprising 298 complexes in total. Up to 87% of the complexes of the Astex diverse set and 77% of the CCDC/Astex clean listnc (noncovalently bound complexes of the clean list) could be reproduced with root-mean-square deviations of less than 2 A with respect to the experimentally determined structures. A comparison with the state-of-the-art docking tool GOLD clearly shows that this is, especially for the druglike Astex diverse set, an improvement in pose prediction performance. Additionally, optimized parameter settings for the search algorithm were identified, which can be used to balance pose prediction reliability and search speed.

  8. Advanced Multi-Product Coal Utilization By-Product Processing Plant

    Energy Technology Data Exchange (ETDEWEB)

    Thomas Robl; John Groppo

    2005-09-01

    The objective of the project is to build a multi-product ash beneficiation plant at Kentucky Utilities 2,200-MW Ghent Generating Station, located in Carroll County, Kentucky. This part of the study includes the examination of the feedstocks for the beneficiation plant. The ash, as produced by the plant, and that stored in the lower pond were examined. A mobile demonstration unit has been designed and constructed for field demonstration. The demonstration unit was hauled to the test site on trailers that were place on a test pad located adjacent to the ash pond and re-assembled. The continuous test unit will be operated at the Ghent site and will evaluate three processing configurations while producing sufficient products to facilitate thorough product testing. The test unit incorporates all of the unit processes that will be used in the commercial design and is self sufficient with respect to water, electricity and processing capabilities. Representative feed ash for the operation of the filed testing unit was excavated from a location within the lower ash pond determined from coring activities. Approximately 150 tons of ash was excavated and pre-screened to remove +3/8 inch material that could cause plugging problems during operation of the demonstration unit.

  9. Recent advances in understanding of meiosis initiation and the apomictic pathway in plants

    Directory of Open Access Journals (Sweden)

    Chung-Ju Rachel Wang

    2014-09-01

    Full Text Available Meiosis, a specialized cell division to produce haploid cells, marks the transition from a sporophytic to a gametophytic generation in the life cycle of plants. In angiosperms, meiosis takes place in sporogenous cells that develop de novo from somatic cells in anthers or ovules. A successful transition from the mitotic cycle to the meiotic program in sporogenous cells is crucial for sexual reproduction. By contrast, when meiosis is bypassed or a mitosis-like division occurs to produce unreduced cells, followed by the development of an embryo sac, clonal seeds can be produced by apomixis, an asexual reproduction pathway found in 400 species of flowering plants. An understanding of the regulation of entry into meiosis and molecular mechanisms of apomictic pathway will provide vital insight into reproduction for plant breeding. Recent findings suggest that AM1/SWI1 may be the key gene for entry into meiosis, and increasing evidence has shown that the apomictic pathway is epigenetically controlled. However, the mechanism for the initiation of meiosis during sexual reproduction or for its omission in the apomictic pathway still remains largely unknown. Here we review the current understanding of meiosis initiation and the apomictic pathway and raised several questions that are awaiting further investigation.

  10. Recent advances in understanding of meiosis initiation and the apomictic pathway in plants.

    Science.gov (United States)

    Wang, Chung-Ju R; Tseng, Ching-Chih

    2014-01-01

    Meiosis, a specialized cell division to produce haploid cells, marks the transition from a sporophytic to a gametophytic generation in the life cycle of plants. In angiosperms, meiosis takes place in sporogenous cells that develop de novo from somatic cells in anthers or ovules. A successful transition from the mitotic cycle to the meiotic program in sporogenous cells is crucial for sexual reproduction. By contrast, when meiosis is bypassed or a mitosis-like division occurs to produce unreduced cells, followed by the development of an embryo sac, clonal seeds can be produced by apomixis, an asexual reproduction pathway found in 400 species of flowering plants. An understanding of the regulation of entry into meiosis and molecular mechanisms of apomictic pathway will provide vital insight into reproduction for plant breeding. Recent findings suggest that AM1/SWI1 may be the key gene for entry into meiosis, and increasing evidence has shown that the apomictic pathway is epigenetically controlled. However, the mechanism for the initiation of meiosis during sexual reproduction or for its omission in the apomictic pathway still remains largely unknown. Here we review the current understanding of meiosis initiation and the apomictic pathway and raised several questions that are awaiting further investigation.

  11. New advanced amine for secondary systems in pressurized water reactor plants

    Energy Technology Data Exchange (ETDEWEB)

    Stevens, Jim [Comanche Peak Steam Electric Station, Glen Rose, TX (United States); Nasrazadani, Seifollah [North Texas Univ., Denton, TX (United States)

    2011-04-15

    Comanche Peak Nuclear Power Plant (CPNPP) augmented morpholine chemistry in the secondary system with dimethylamine (DMA) chemistry after discovering the benefits from Italian fossil plant experience. As a result of the operational success with DMA, CPNPP began searching for other amines with similar properties that may provide greater benefit. The amine discovered to have the most favorable potential benefits was 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), which is a strong base with a pKa of 13.4. Corrosion and compatibility testing was performed in reference to existing amines and followed the pattern of the qualification testing performed for the implementation of DMA. This work describes the candidate testing that led to the selection of DBU and the qualification testing for operation at CPNPP. Testing was performed on carbon steel for balance of plant considerations and on Alloy 600 steam generator tubing material. Also described is an unexpected finding from the testing. One test regime indicated a corrosion protection benefit of DBU beyond a simple pH effect, which may be the result of surface modification or surface reaction with the carbon steel oxide. (orig.)

  12. Recent advances in utilizing transcription factors to improve plant abiotic stress tolerance by transgenic technology

    Directory of Open Access Journals (Sweden)

    Hongy