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Sample records for piruvato deshidrogenasa dn

  1. Glutamato deshidrogenasas en cianobacterias

    OpenAIRE

    Chávez de Diego, Sebastián

    1992-01-01

    La finalidad del trabajo que aquí se presenta es contribuir al conocimiento de la función desarrollada por la actividad glutamato deshidrogenasa en las cianobacterias y de su relación con la asimilación de nitrógeno inorgánico, así como profundizar en las características moleculares de estas enzimas. Para ello se ha empleado una amplia metodología que abarca desde las aproximaciones puramente fisiológicas hasta el análisis filogenético de las secuencias de aminoácidos. El estudio físico-quími...

  2. PARTICIPACION DEL COMPLEJO PIRUVATO DESHIDROGENASA DE AEROMONAS CAVIAE ST EN RESISTENCIA TELURITO DE POSTASIO (K2TEO3)

    OpenAIRE

    2008-01-01

    El telurito de potasio (K2TeO3) es altamente tóxico para la mayoría de los seres vivos. Aunque se desconoce el mecanismo de toxicidad especifico, recientemente se ha demostrado que estaría dado, al menos en parte, por el establecimiento de un estrés oxidativo. Aeromonas caviae ST, el microorganismo utilizado en este estudio es altamente resistente a K2TeO3 y extractos crudos de esta bacteria catalizan la reducción de K2TeO3 in vitro. Se obtuvo fracciones con actividad telurito reductasa qu...

  3. Favismo: deficiencia de glucosa - 6- fosfato deshidrogenasa

    OpenAIRE

    Díez de Fuentes, Sara

    2016-01-01

    Favismo es una anomalía de carácter hereditario responsable de la hemólisis aguda que se produce cuando los individuos que la padecen entran en contacto con vicina y convicina, beta-glucósidos con alto poder oxidativo contenidos en Vicia Faba. Favismo es consecuencia de un déficit de una de las enzimas de la ruta de las pentosas-fosfato, glucosa-6-fosfato deshidrogenasa (G-6PDH), ocasionado por mutaciones en la secuencia aminoacídica de la misma. La ruta de las pentosas – fosfato es una impor...

  4. Síntesis de las enzimas de piruvato, oxidorreductasa de ferredoxina y deshidrogenasa E de alcohol durante el desenquistamiento (excystacion de Giardia intestinalis

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Niño

    2010-03-01

    Conclusiones. Nuestros resultados muestran de forma concluyente que las enzimas PFOR y ADHE son traducidas en el proceso de desenquistamiento (excystacion y esto demuestra que existe un proceso activo de síntesis de proteínas durante él.

  5. Expressão heteróloga da forma selvagem e mutante da piruvato desidrogenase humana (hPDHE1): efeito de chaperones químicos

    OpenAIRE

    Florindo, Cristina Isabel Pereira

    2010-01-01

    Tese de mestrado em Bioquímica (Bioquímica Médica), apresentada à Universidade de Lisboa, através da Faculdade de Ciências, 2010 O sistema piruvato desidrogenase humano (PDHc) e um complexo multienzimático, localizado na matriz mitocondrial, que apresenta uma função essencial no metabolismo da glucose ao promover a descarboxilação oxidativa do piruvato a acetil-CoA. De entre os seus constituintes, e de destacar o piruvato desidrogenase (E1; EC 1.2.4.1), uma proteína heterodimérica (α2β2) q...

  6. Fuchsian equations of type DN

    NARCIS (Netherlands)

    Golyshev, V.

    2007-01-01

    We prove that a generic differential operator of type DN is irreducible, regular, (anti)self-adjoint, and has quasiunipotent local monodromies. We prove that the defining matrix of a DN operator can be recovered from the expression of the operator as a polynomial in t and d/dt. -

  7. Deficiencia de glucosa-6-fosfato deshidrogenasa en un paciente con síndrome de Down

    Directory of Open Access Journals (Sweden)

    Francisco R. Cammarata Scalisi

    2012-07-01

    Full Text Available El síndrome de Down, es una alteración genética que ocurre cuando un individuo exhibe todo o una parte específica adicional del cromosoma 21 y es la entidad más frecuentemente asociada a retardo mental. La deficiencia de glucosa-6-fosfato deshidrogenasa, es el defecto enzimático más común en humanos y presenta patrón de herencia ligado al cromosoma X recesivo. Se debe a la mutación del gen G6PD, el cual causa diversos fenotipos bioquímicos y clínicos. Reportamos un caso de lactante menor masculino, evaluado en la Unidad de Genética Médica de la Universidad de Los Andes, con el diagnóstico de deficiencia de glucosa-6-fosfato deshidrogenasa con doble mutación A376G y G202A y síndrome de Down con estudio citogenético 47, XY, +21. Palabras clave:Síndrome de Down; deficiencia de glucosa-6-fosfato deshidrogenasa; G6PD; A37G6; G202A. Glucose-6-phosphate dehydrogenase deficiency in a patient with Down syndrome Abstract Down syndrome, is a genetic disorder that occurring when an individual exhibits all or part of an extra copy of chromosome 21 and the most common entity associated mental retardation. Glucose-6-phosphate dehydrogenase deficiency, is the most common human enzyme defect and has a X-linked recessive inheritance. Due to mutations in the G6PD gene, which cause many biochemical and clinical phenotypes. We reported a case of child male, evaluated in the Unit of Medical Genetics of the University of The Andes, with diagnosis of glucose-6-phosphate dehydrogenase deficiency with double mutation A376G and G202A and Down syndrome with cytogenetic study 47, XY, + 21.

  8. DN interaction from meson exchange

    NARCIS (Netherlands)

    Haidenbauer, J.; Krein, G.; Meiner, U. -G.; Tolos, L.

    A model of the DN interaction is presented which is developed in close analogy to the meson-exchange (K) over barN potential of the Julich group utilizing SU(4) symmetry constraints. The main ingredients of the interaction are provided by vector meson (rho, omega) exchange and higher-order box

  9. Actividad enzimática del suelo -Deshidrogenasa , -ß Glucosidasa , Fosfatasa y Ureasa - bajo diferentes cultivos.

    Directory of Open Access Journals (Sweden)

    Carlos Henr\\u00EDquez

    2014-01-01

    Full Text Available Se analizó la actividad de 4 enzimas del suelo en fincas bajo diferentes manejos agronómi - cos y en diferentes tipos de suelos. Las enzimas evaluadas fueron la Fosfatasa, β-Glucosidasa, Deshidrogenasa y Ureasa. Se tomaron muestras de suelo de los primeros 20 cm de profundidad con el fin de analizarlas químicamente y determinar la actividad de las 4 enzimas a evaluar. Se encontró que la actividad de la Deshidrogenasa varió de 0,13 a 4,46 con un promedio de 1,17 (μg INTF.g - 1 .h -1 ; la β-Glucosidasa varió de 31,9 a 208,1 con un promedio de 108,1 (μg PNP.g -1 .h -1 . Por otro lado la Fosfatasa tuvo valores entre 413,4 y 3043,6 con un promedio de 1521,5 μg PNP.g -1 .h -1 , mientras que la Ureasa varió de 12,5 a 52,8 con un promedio de 38,3 μg N-NH4.g -1 .h -1 . La Fos - fatasa correlacionó con el porcentaje de materia orgánica y de carbono en el suelo (0,75 y 0,76 respectivamente, p<0,01; se encontró una rela - ción inversa entre la actividad de esta enzima y el contenido de P disponible (r de -0,53, p<0,10. La Ureasa correlacionó con la Fosfatasa (r de 0,61 p<0,05 y con la Deshidrogenasa (r de -0,77 p<0,01. El análisis multivariado de conglomera - dos a partir de la actividad enzimática permitió hacer grupos similares según el tipo de cultivo, lo que evidencia el potencial de utilización que tiene esta propiedad para realizar estudios más detallados.

  10. Hemolytic anemia caused by hereditary pyruvate kinase deficiency in a West Highland White Terrier dog=Anemia hemolítica causada por la deficiencia de piruvato quinasa hereditaria en un perro West Highland White Terrier

    OpenAIRE

    Hlavac,Nicole Regina Capacchi; Lacerda,Luciana de Almeida; Conrado, Francisco de Oliveira; Hünning, Paula Stieven; Seibert, M.; Diaz Gonzalez, Félix Hilário; Giger, U.

    2012-01-01

    La deficiencia de piruvato quinasa (PK) es un desorden hemolítico autosómico recesivo descrito en perros y gatos. La piruvato quinasa es una de las enzimas regulatorias esenciales de la glicólisis anaeróbica, la deficiencia de esta enzima causa una destrucción prematura de los eritrocitos. El presente es un estudio de caso y relata los hallazgos clínicos y paraclínicos en un perro brasileño de la raza West Highland White Terrier (WHWT) con historia de debilidad e intolerancia al ejercicio. El...

  11. Deficiencia de glucosa-6-fosfato deshidrogenasa en un paciente con síndrome de Down

    OpenAIRE

    Francisco R. Cammarata Scalisi; Harry Sánchez; Graciela Cammarata Scalisi; Nayra Cabral Alfonso; Osmoire Moreno; Miguel Alonzo Bastardo Ramos; Gustavo Gil

    2012-01-01

    El síndrome de Down, es una alteración genética que ocurre cuando un individuo exhibe todo o una parte específica adicional del cromosoma 21 y es la entidad más frecuentemente asociada a retardo mental. La deficiencia de glucosa-6-fosfato deshidrogenasa, es el defecto enzimático más común en humanos y presenta patrón de herencia ligado al cromosoma X recesivo. Se debe a la mutación del gen G6PD, el cual causa diversos fenotipos bioquímicos y clínicos. Reportamos un caso de lactante menor masc...

  12. Deshidrogenasa láctica sérica en preeclámpticas y embarazadas normotensas

    Directory of Open Access Journals (Sweden)

    Avelin Vargas-García

    2016-08-01

    Full Text Available El objetivo de la investigación fue comparar las concentraciones séricas de deshidrogenasa láctica en pacientes con preeclampsia y embarazadas normotensas sanas. Se seleccionó un total de 180 pacientes. Se incluyeron 90 preeclámpticas como grupo de estudio (grupo A y un grupo de control seleccionado por tener edad e índice de masa corporal similares al grupo de estudio que consistió en 90 embarazadas normotensas sanas (grupo B. Las muestras de sangre se recolectaron en todas las pacientes antes del parto e inmediatamente después del diagnóstico en el grupo A para determinar las concentraciones de deshidrogenasa láctica. Se encontraron diferencias estadísticamente significativas en las concentraciones séricas de deshidrogenasa láctica entre las pacientes del grupo de estudio (grupo A: 523,9 +/- 131,7 UI/L y las pacientes del grupo de control (grupo B: 241,0 +/- 76,3 UI/L; p < 0,001. No se observaron correlaciones significativas con los valores de presión arterial sistólica y diastólica (p = ns. Un valor de corte de 350 UI/L presentó un valor por debajo de la curva de 0,98, sensibilidad del 92,2%, especificidad del 86,6%, valor predictivo positivo del 87,7% y valor predictivo negativo del 91,7%, con una exactitud diagnóstica del 89,4%. Se concluye que las preeclámpticas presentaron concentraciones séricas significativamente más altas de deshidrogenasa láctica al compararlo con embarazadas normotensas sanas

  13. INHIBICION DE LA EXPRESION DEL GEN DE LA DESHIDROGENASA ALDEHIDICA: EVALUACION DEL SISTEMA DE RNA DE INTERFERENCIA

    OpenAIRE

    CORTINEZ FLORES, GABRIEL

    2008-01-01

    El alcoholismo es una enfermedad multifactorial que genera una serie de trastornos personales, sociales y de salud pública. Según estimaciones del Ministerio de Salud de Chile el abuso en el consumo de alcohol y el alcoholismo causa pérdidas al país por US$3.000 millones anuales. Luego de la oxidación hepática del etanol, el acetaldehído generado es metabolizado a acetato por la deshidrogenasa aldehídica 2 (ALDH2*1). En la población Asiática existen individuos que poseen una variante de la...

  14. Diagnostico De La Deficiencia De Acil-Coa Deshidrogenasa De Cadena Muy Larga Mediante El Uso De Sustratos Tritiados

    OpenAIRE

    Osorio, José Henry; Laboratorio de Patología Molecular, Departamento de Ciencias Básicas de la Salud. Universidad de Caldas. e.mail:; Ribes, Antonia; Instituto de Bioquímica Clínica. Corporació Sanitaria Clínic. Barcelona. España Correspondencia: Universidad de Caldas, Departamento de Ciencias Básicas de la Salud. Calle 65 N0. 26-10. Manizales; Lluc, Montse; Instituto de Bioquímica Clínica. Corporació Sanitaria Clínic. Barcelona. España Correspondencia: Universidad de Caldas, Departamento de Ciencias Básicas de la Salud. Calle 65 N0. 26-10. Manizales

    2008-01-01

    Introducción: la acil-CoA deshidrogenasa de cadena muy larga (VLCAD) cataliza la primera reacción de la degradación de ácidos grasos de 14 a 24 átomos de carbono. Su deficiencia debe ser siempre confirmada por estudios de laboratorio. Materiales y métodos: en el presente trabajo, fueron incubados fibroblastos de pacientes que presentaban la deficiencia de VLCAD, en presencia de sustratos tritiados. Resultados y discusión: Fue encontrada diferencia significativa (P<0.05) al comparar la degr...

  15. Deficiencia de glucosa-6-fosfato deshidrogenasa: De lo clínico a lo bioquímico

    OpenAIRE

    Saúl Gómez-Manzo; Gabriel López-Velázquez; Itzhel García-Torres; Gloria Hernández-Alcantara; Sara Teresa Méndez-Cruz; Jaime Marcial-Quino; Adriana Castillo-Villanueva; Sergio Enríquez-Flores; Ignacio De la Mora; Angélica Torres-Arroyo; Horacio Reyes-Vivas; Jesús Oria-Hernández

    2014-01-01

    La deficiencia de Glucosa-6-fosfato deshidrogenasa (G6PD) es la enzimopatía más frecuente, con una prevalencia global del 4,9% y con alrededor de 330 a 400 millones de personas afectadas en el mundo. La G6PD desempeña un papel fundamental en el equilibrio redox intracelular, especialmente en los eritrocitos; en condiciones de estrés oxidativo inducido (por ejemplo, por exposición a agentes externos como fármacos, alimentos o infecciones), los hematíes portadores de la variante enzimática y...

  16. Maturation of auditory brainstem projections and calyces in the congenitally deaf (dn/dn) mouse.

    Science.gov (United States)

    Youssoufian, Monique; Couchman, Kiri; Shivdasani, Mohit N; Paolini, Antonio G; Walmsley, Bruce

    2008-01-20

    The deaf dn/dn mouse is a valuable model of human congenital deafness. In this study we used the lipophylic dye DiA to trace auditory nerve and cochlear nucleus projections in the dn/dn mouse. In both normal and deaf mice, the ipsilateral projections from the anteroventral cochlear nucleus (AVCN) to the lateral superior olive (LSO), and the contralateral projections from the AVCN to the medial nucleus of the trapezoid body (MNTB) were intact. With age, there was a noted increase in the fenestration of the endbulb and calyx of Held, and this morphological maturation was also observed in the deaf mice, although there was a significant difference in total endbulb volume at P20 between normal and deaf mice. However, total calyceal volume was not significantly different between normal and deaf mice. There was electrophysiological evidence of in vivo spontaneous ventral cochlear nucleus activity in normal and deaf animals, indicating that this activity may be responsible for the appropriate connectivity in the deaf mice. Our results indicate that congenital deafness caused by the dn/dn mutation does not result in aberrant projections between the AVCN and the ipsilateral MNTB and contralateral LSO but can cause abnormalities in endbulb size.

  17. Tinkertoys for the D_N series

    CERN Document Server

    Chacaltana, Oscar

    2013-01-01

    We describe a procedure for classifying 4D N=2 superconformal theories of the type introduced by Davide Gaiotto. Any punctured curve, C, on which the 6D (2,0) SCFT is compactified, may be decomposed into 3-punctured spheres, connected by cylinders. The 4D theories, which arise, can be characterized by listing the "matter" theories corresponding to 3-punctured spheres, the simple gauge group factors, corresponding to cylinders, and the rules for connecting these ingredients together. Different pants decompositions of $ correspond to different S-duality frames for the same underlying family of 4D N=2 SCFTs. In a previous work [1], we developed such a classification for the A_{N-1} series of 6D (2,0) theories. In the present paper, we extend this to the D_N series. We outline the procedure for general D_N, and construct, in detail, the classification through D_4. We discuss the implications for S-duality in Spin(8) and Spin(7) gauge theory, and recover many of the dualities conjectured by Argyres and Wittig [2].

  18. EST Table: DN985252 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN985252 EST01098 11/12/09 GO hit GO:0016021(integral to membrane) 10/09/28 56 %/13...10289-PA 10/09/10 53 %/125 aa gi|91094217|ref|XP_973172.1| PREDICTED: similar to AGAP001778-PA [Tribolium castaneum] DN985252 BmP ...

  19. EST Table: DN237285 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237285 EST00410 10/09/29 42 %/121 aa ref|YP_015696.1| polyprotein [Kakugo virus] ...dbj|BAD06930.1| polyprotein [Kakugo virus] 10/09/02 n.h 10/08/28 n.h 10/09/10 n.h 10/09/10 n.h 10/09/10 n.h DN237490 BmP ...

  20. EST Table: DN237513 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237513 EST00639 10/09/29 45 %/111 aa ref|YP_145791.1| polyprotein [Varroa destruc...tor virus 1] gb|AAP51418.2| polyprotein [Varroa destructor virus 1] 10/09/03 n.h 10/08/28 n.h 10/09/10 n.h 10/09/10 n.h 10/09/10 n.h DN237490 BmP ...

  1. EST Table: DN236936 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN236936 EST00061 11/12/09 n.h 10/09/29 54 %/228 aa sp|Q25490.1|APLP_MANSE RecName:...9/02 n.h 10/08/28 n.h 10/09/10 n.h 10/09/10 low homology 10/09/10 low homology DN236936 BmP ...

  2. Hemolytic anemia caused by hereditary pyruvate kinase deficiency in a West Highland White Terrier dog Anemia hemolítica causada por la deficiencia de piruvato quinasa hereditaria en un perro West Highland White Terrier

    Directory of Open Access Journals (Sweden)

    NRC Hlavac

    2012-01-01

    Full Text Available Inherited erythrocytic pyruvate kinase (PK deficiency is an autosomal recessive hemolytic disorder described in dogs and cats. Pyruvate kinase is one of two key-regulatory enzymes in the anaerobic glycolytic pathway, and its deficiency causes premature destruction of the ATP-depleted red cells. This case study reports the clinical and laboratory findings in a West Highland White Terrier (WHWT dog with a life-long history of weakness and exercise intolerance from Brazil. The dog was found to have persistently profound pallor, a highly regenerative hemolytic anemia and osteosclerosis. PK deficiency was confirmed by a breed-specific DNA test for the previously described 6 bp insertion at 3' end of exon 10 in red blood cell (RBC R- PK gene sequence. The dog was euthanized at 20 months of age due to the deterioration of its clinical condition including anemia and blood incompatibility. Other PK-deficient WHWTs have lived as long as 9 years. Hereditary red cell defects are important differential diagnoses for chronic hemolytic anemias in younger animals after excluding immune-mediated and infectious causes. Furthermore, purebred dogs for which DNA tests for hereditary diseases are available should be screened prior to breeding in order to limit the spread of the mutant allele and to avoid future production of PK-deficient animals.La deficiencia de piruvato quinasa (PK es un desorden hemolítico autosómico recesivo descrito en perros y gatos. La piruvato quinasa es una de las enzimas regulatorias esenciales de la glicólisis anaeróbica, la deficiencia de esta enzima causa una destrucción prematura de los eritrocitos. El presente es un estudio de caso y relata los hallazgos clínicos y paraclínicos en un perro brasileño de la raza West Highland White Terrier (WHWT con historia de debilidad e intolerancia al ejercicio. El paciente presentaba mucosas pálidas, anemia hemolítica bastante regenerativa y osteoclerosis. La deficiencia de PK fue

  3. Pyruvate kinase activation and lipoperoxidation after selective hepatic ischemia in Wistar rats Ativação da piruvato quinase e lipoperoxidação após isquemia hepática seletiva em ratos Wistar

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    Ilka de Fátima Santana Ferreira Boin

    2006-01-01

    Full Text Available PURPOSE: Hepatic ischemia and reperfusion can cause several problems in hepatic surgery. The aim of this study was to determine pyruvate kinase activation and lipid peroxidation after hepatic ischemia. METHODS: Twenty-four Wistar rats were submitted to 90 minutes of selective liver ischemia and 15 minutes of reperfusion. Twelve animals were submitted to selective liver ischemia and reperfusion (Group A and the other 12 were submitted to sham operation (Group B. After 15 minutes of reperfusion, the following parameters were measured: mean arterial pressure (MAP, alanine aminotransferase (ALT, glycemia (GLY, hepatic glycogen (GH, pyruvate kinase (PK activation, hepatic glutathione (GSH and malondialdehyde (MDA. Analysis of the results were made by the Student t-test and has been considered significant difference for pOBJETIVO: A isquemia e reperfusão hepática podem causar graves repercussões hepatocelulares em cirurgias hepáticas. O objetivo deste estudo foi determinar o comportamento da piruvato EM PORTUGUÊS quinase e a lipoperoxidação após isquemia hepática. MÉTODOS: Foram utilizados vinte e quatro ratos Wistar machos divididos em dois grupos. Doze animais foram submetidos a 90 minutos de isquemia hepática seletiva e reperfusão hepática de por 15 minutos (pressão arterial média (PAM, alanina aminotransferase (ALT, glicemia (GLI, gicogênio hepático (GH, ativação da piruvato quinase (PQ, glutationa hepática (GSH e malondialdeído (MDA. Os resultados foram analisados utilizando o teste t de Student sendo as diferenças consideradas significativas para p<0,05. RESULTADOS: Verificou-se diferença significativa entre os grupos em todos os parâmetros analisados. CONCLUSÃO: Verificou-se que os animais do grupo A mostraram síndrome de reperfusão com queda da PAM, ativação do metabolismo da glicose através da via glicolítica e presença de lipoperoxidação quando comparada com o grupo B.

  4. Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2

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    Dnyanada S. Khanolkar

    2014-12-01

    Full Text Available A bacterial isolate capable of utilizing tributyltin chloride (TBTCl as sole carbon source was isolated from estuarine sediments of west coast of India and identified as Pseudomonas stutzeri based on biochemical tests and Fatty acid methyl ester (FAME analysis. This isolate was designated as strain DN2. Although this bacterial isolate could resist up to 3 mM TBTCl level, it showed maximum growth at 2 mM TBTCl in mineral salt medium (MSM. Pseudomonas stutzeri DN2 exposed to 2 mM TBTCl revealed significant alteration in cell morphology as elongation and shrinkage in cell size along with roughness of cell surface. FTIR and NMR analysis of TBTCl degradation product extracted using chloroform and purified using column chromatography clearly revealed biotransformation of TBTCl into Dibutyltin dichloride (DBTCl2 through debutylation process. Therefore, Pseudomonas stutzeri strain DN2 may be used as a potential bacterial strain for bioremediation of TBTCl contaminated aquatic environmental sites.

  5. Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2

    Science.gov (United States)

    Khanolkar, Dnyanada S.; Naik, Milind Mohan; Dubey, Santosh Kumar

    2014-01-01

    A bacterial isolate capable of utilizing tributyltin chloride (TBTCl) as sole carbon source was isolated from estuarine sediments of west coast of India and identified as Pseudomonas stutzeri based on biochemical tests and Fatty acid methyl ester (FAME) analysis. This isolate was designated as strain DN2. Although this bacterial isolate could resist up to 3 mM TBTCl level, it showed maximum growth at 2 mM TBTCl in mineral salt medium (MSM). Pseudomonas stutzeri DN2 exposed to 2 mM TBTCl revealed significant alteration in cell morphology as elongation and shrinkage in cell size along with roughness of cell surface. FTIR and NMR analysis of TBTCl degradation product extracted using chloroform and purified using column chromatography clearly revealed biotransformation of TBTCl into Dibutyltin dichloride (DBTCl2) through debutylation process. Therefore, Pseudomonas stutzeri strain DN2 may be used as a potential bacterial strain for bioremediation of TBTCl contaminated aquatic environmental sites. PMID:25763027

  6. EST Table: DN237676 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237676 EST00802 10/09/29 99 %/257 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  7. EST Table: DN237681 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237681 EST00807 10/09/29 99 %/232 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  8. EST Table: DN237717 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237717 EST00843 10/09/29 100 %/117 aa ref|NP_001037309.1| vitellogenin precursor ...gs: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  9. EST Table: DN237719 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237719 EST00845 10/09/29 99 %/200 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  10. EST Table: DN237655 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237655 EST00781 10/09/29 95 %/259 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  11. EST Table: DN237682 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237682 EST00808 10/09/29 99 %/228 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  12. EST Table: DN237714 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237714 EST00840 10/09/29 99 %/253 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  13. EST Table: DN237644 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237644 EST00770 10/09/29 99 %/227 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  14. EST Table: DN237706 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237706 EST00832 10/09/29 99 %/210 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  15. EST Table: DN237346 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237346 EST00472 10/09/29 97 %/120 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  16. EST Table: DN237656 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237656 EST00782 10/09/29 91 %/268 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  17. EST Table: DN237703 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237703 EST00829 10/09/29 99 %/231 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  18. EST Table: DN237718 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237718 EST00844 10/09/29 99 %/202 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  19. EST Table: DN237662 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237662 EST00788 10/09/29 89 %/271 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  20. EST Table: DN237680 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237680 EST00806 10/09/29 95 %/227 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  1. EST Table: DN237338 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237338 EST00464 10/09/29 96 %/116 aa ref|NP_001037309.1| vitellogenin precursor [...s: Precursor dbj|BAA02444.1| vitellogenin precursor [Bombyx mori] dbj|BAA06397.1| vitellogenin [Bombyx mori

  2. Data of evolutionary structure change: 1CG8A-2DN1B [Confc[Archive

    Lifescience Database Archive (English)

    Full Text Available equence>FSGFE-----ACNEQ ure> ----- HH> B 2DN1B FGDLSTPDAVMGNPK re>G HHHH HH...ence>VNLQA-FTPVT >HH - HHH> 2DN1 B 2DN1B HHFGKEFTPPV ...>HHHGGG HHH> ATOM 1931 CA HIS B 116 17.758 39.

  3. EST Table: DN237490 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available 019079(viral genome replication) 10/09/29 44 %/102 aa ref|YP_145791.1| polyprotein [Varroa destructor virus ...1] gb|AAP51418.2| polyprotein [Varroa destructor virus 1] 10/09/03 n.h 10/08/28 n.h 10/09/10 n.h 10/09/10 n.h 10/09/10 n.h DN237490 BmP ...

  4. Condensation pool experiments with steam using DN200 blowdown pipe

    Energy Technology Data Exchange (ETDEWEB)

    Laine, J.; Puustinen, M. [Lappeenranta Univ. of Technology (Finland)

    2005-08-01

    This report summarizes the results of the condensation pool experiments with steam using a DN200 blowdown pipe. Altogether five experiment series, each consisting of several steam blows, were carried out in December 2004 with a scaled-down test facility designed and constructed at Lappeenranta University of Technology. The main purpose of the experiments was to increase the understanding of different phenomena in the condensation pool during steam discharge. (au)

  5. Leaching of DOC, DN, and inorganic constituents from scrap tires.

    Science.gov (United States)

    Selbes, Meric; Yilmaz, Ozge; Khan, Abdul A; Karanfil, Tanju

    2015-11-01

    One concern for recycle and reuse of scrap tires is the leaching of tire constituents (organic and inorganic) with time, and their subsequent potential harmful impacts in environment. The main objective of this study was to examine the leaching of dissolved organic carbon (DOC), dissolved nitrogen (DN), and selected inorganic constituents from scrap tires. Different sizes of tire chips and crumb rubber were exposed to leaching solutions with pH's ranging from 3.0 to 10.0 for 28days. The leaching of DOC and DN were found to be higher for smaller size tire chips; however, the leaching of inorganic constituents was independent of the size. In general, basic pH conditions increased the leaching of DOC and DN, whereas acidic pH conditions led to elevated concentrations of metals. Leaching was minimal around the neutral pH values for all the monitored parameters. Analysis of the leaching rates showed that components associated with the rubbery portion of the tires (DOC, DN, zinc, calcium, magnesium, etc.) exhibited an initial rapid followed by a slow release. On the other hand, a constant rate of leaching was observed for iron and manganese, which are attributed to the metal wires present inside the tires. Although the total amounts that leached varied, the observed leaching rates were similar for all tire chip sizes and leaching solutions. Operation under neutral pH conditions, use of larger size tire chips, prewashing of tires, and removal of metal wires prior to application will reduce the impact of tire recycle and reuse. Copyright © 2015 Elsevier Ltd. All rights reserved.

  6. Computation of a Specific Determinant Dn(m,k)%特殊行列式Dn(m,k)的计算

    Institute of Scientific and Technical Information of China (English)

    王念良; 严小红

    2001-01-01

    给出了计算以数列{Pn}的项为元素的特殊行列式Dn(m,k)的一般公式.以及数列{Pn}一般项由递推公式Pn+1(x)=s(x)Pn(x)+t(x)Pn-1(x)确定时,求数列一般项的公式,并讨论了当Pn=ncλn+P0λn(c,λ,P0为常数)且m<n-1时,Dn(m,k)=0的重要性质,最后指出Fibonacci,chebyshev行列式的计算,仅是本命题的一个特例.%The general formula is given for calculating the specific determinant, whose elements are the terms of a number sequence {Pn}. The formula is also given for calculating the terms when the terms are determined by the recursion formula of Pn+1(x)=S(x)+Pn-1(x)*t(x). The important characteristics of Dn(m,k)=0 were discussed when Pn=ncλn+Poλn(c,λ,Po are all constants) and m<n-1.It is pointed that Fibonacci's and chebyshev's determinants are two specific examples of the proposition.

  7. Actividad de la lactato deshidrogenasa en fluído crevicular gingival y saliva en fumadores con periodontitis crónica Lactate dehidrogenase activity in gingival crevicular fluid and saliva of smoker with chronic periodontitis

    OpenAIRE

    E.R. Leyva Huerta; C. Esquivel Chirino; G. Marín González; M. Neblina Noriega; S. Olivares Tapia

    2009-01-01

    Existe un alto porcentaje de enfermedad periodontal atribuido al hábito del tabaco. La respuesta del organismo a esta enfermedad incluye la liberación de enzimas intracelulares relacionadas con la muerte y destrucción celular, como la Lactato Deshidrogenasa (LDH). Objetivo: Comparar los valores de LDH en fluido crevicular gingival (FCG) y saliva de pacientes fumadores y no fumadores con Periodontitis crónica (PC). Metodología: Participaron 15 pacientes hombres mayores de 30 años; 6 fumadores,...

  8. Glucosa-6-fosfato deshidrogenasa (G6PD). Respuesta de los hematíes y otras células humanas a la disminución en su actividad

    OpenAIRE

    Javier Fernando Bonilla; Magda Carolina Sánchez; Lilian Chuaire

    2007-01-01

    La glucosa-6-fosfato deshidrogenasa (G6PD) es la primera enzima de la vía pentosa fosfato y la principal fuente intracelular de nicotidamina adenina dinucleótido fosfato reducido (NADPH), compuesto comprometido en diversos procesos fisiológicos, por ejemplo defensa antioxidante (sobre todo células como los eritrocitos), modulación del crecimiento endotelial, eritropoyesis, vascularización y fagocitosis. La deficiencia de G6PD es la enzimopatía ligada al cromosoma X más común en el ser human...

  9. EST Table: DN985187 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN985187 EST01033 10/09/28 51 %/157 aa gb|AAP42818.1| NADH dehydrogenase subunit 6 [Bombyx mandarin...a] gb|ADE18271.1| NADH dehydrogenase subunit 6 [Bombyx mandarina] gb|ADE18284.1| NADH dehydr...ogenase subunit 6 [Bombyx mandarina] gb|ADE18388.1| NADH dehydrogenase subunit 6 [Bombyx mandarina] gb|ADE18...557.1| NADH dehydrogenase subunit 6 [Bombyx mandarina] gb|ADE18583.1| NADH dehydr...ogenase subunit 6 [Bombyx mandarina] gb|ADE18661.1| NADH dehydrogenase subunit 6 [Bombyx mandarina] gb|ADE18

  10. EST Table: DN237519 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available DN237519 EST00645 10/09/29 49 %/159 aa gb|AAP42818.1| NADH dehydrogenase subunit 6 [Bombyx mandarin...a] gb|ADE18271.1| NADH dehydrogenase subunit 6 [Bombyx mandarina] gb|ADE18284.1| NADH dehydr...ogenase subunit 6 [Bombyx mandarina] gb|ADE18388.1| NADH dehydrogenase subunit 6 [Bombyx mandarina] gb|ADE18...557.1| NADH dehydrogenase subunit 6 [Bombyx mandarina] gb|ADE18583.1| NADH dehydr...ogenase subunit 6 [Bombyx mandarina] gb|ADE18661.1| NADH dehydrogenase subunit 6 [Bombyx mandarina] gb|ADE18

  11. Las quinoproteínas alcohol deshidrogenasas en los sistemas bacterianos: distribución, clasificación, estructura y función

    Directory of Open Access Journals (Sweden)

    Saúl Gómez-Manzo

    2005-01-01

    Full Text Available Existe una gran diversidad de alcohol deshidrogenasas (ADHs microbianas; las cuales son divididas en tres grandes grupos: (a Las que son dependientes de las coenzimas NAD o NADP, (b Las que son independientes de estas coenzimas; sin embargo, utilizan pirroloquinolina quinona (PQQ y hemo tipo C como grupo prostético y (c Las oxidasas dependientes de FAD que catalizan la reacción irreversible de alcoholes. Las ADHs que utilizan el PQQ, se encuentran a su vez divididas en tres tipos. Las ADHs tipo I que contienen sólo PQQ como grupo prostético y se les conoce como quinoproteínas; mientras que las ADHs tipo II y tipo III además del PQQ contienen hemo tipo C y se les conoce como quinohemoproteínas. Las ADHs tipo II son enzimas solubles que se encuentran en el espacio periplásmico y están presentes en proteobacterias como Pseudomonas putida, Ralstonia eutropha y Comamonas testosteroni. Las ADHs tipo III son enzimas que se encuentran ancladas a la membrana y trabajan orientadas hacia el espacio periplásmico. Se les ha identificado y caracterizado únicamente en bacterias ácido acéticas. Las ADH tipo III, por lo general contienen tres subunidades. El transporte intramolecular de electrones en las ADHs tipo II y IIII se propone que es del PQQ al hemo C de la primera subunidad y de ahí, de hemo en hemo en la segunda subunidad hasta llegar a la quinona endógena. Los tres tipos de PQQ-ADHs son discutidas en esta revisión.

  12. On simple modules of Hecke algebras of type Dn

    Institute of Scientific and Technical Information of China (English)

    HU; Jun(

    2001-01-01

    [1]Hu Jun, Wang Jianpan, Hecke algebras of type Dn at roots of unity, J. Alg. , 1999, 212: 132-160.[2]Pallikaros, C., Representations of Hecke algebras of type Dn, J. Alg., 1994, 169: 20-48.[3]Ford, B., Irreducible representations of the alternating group in odd characteristic, Proc. Amer. Math. Soc. , 1997, 125 (2): 375-380.[4]Geck, M., Kazhdan-Lusztig cells and decomposition numbers, Represent. Theory, 1998, 2:264-277 (electronic).[5]Dipper R., James, G. D., Representations of Hecke algebras of type Bn, J. Alg., 1992, 146: 454-481.[6]Graham J. J., Lehrer, G. I., Cellular algebras, Invent. Math., 1996, 123: 1-34.[7]Dipper, R., James G. D., Murphy, E., Hecke algebras of type Bn at roots of unity, Proc. London. Math. Soc., 1995, 70 (3): 505-528.[8]Halverson, T., Ram, A., Mumaghan-Nakayama rules characters of Iwahori-Hecke algebras of the complex reflection groups G(r,p,n), Can. J. Math. ,1997, 50(1): 167-192.[9]Ram, A., Seminormal representations of Weyl groups and Iwahori-Hecke algebras, Proc. London. Math. Soc., 1997, 75 (1): 99-133.

  13. Efecto de la aplicación de vinazas en las propiedades físicas y la actividad de deshidrogenasas en suelos cultivados con maíz dulce (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Magda Narváez Castillo

    2010-04-01

    Full Text Available Se evaluó el efecto de la aplicación de vinazas en algunas propiedades físicas y en la actividad de las deshidrogenasas en un Entic dystropept y en un Fluventic haplustoll del Valle de Cauca, Colombia. Para suplir los requerimientos de K+ del cultivo de maíz dulce (Zea mays L. se utilizaron seis tratamientos (T1 = 100% de K+ como vinaza, T2 = 100% de K+ con KCl; T3 = 50% de K+ como vinaza y KCl, T4 = 75% de K+ con vinaza y 25% de K+ como KCl, T5 = testigo absoluto sin planta, T6 = testigo más planta que fueron dispuestos en un diseño completamente al azar con cinco repeticiones. En el Entic dystropept la densidad aparente disminuyó y en consecuencia la porosidad total aumentó, mientras que en el Fluventic haplustoll sucedió lo contrario. La actividad de las deshidrogenasas fue significativamente mayor (P < 0.05 en el Entic dystropept que en el Fluventic haplustoll que presentó valores bajos. Los resultados mostraron que los cambios que se generan como producto de la aplicación de vinazas son diferentes para ambos tipo de suelo.

  14. Efecto de la aplicación de vinazas en las propiedades físicas y la actividad de deshidrogenasas en suelos cultivados con maíz dulce (Zea mays L.

    Directory of Open Access Journals (Sweden)

    Narváez Castillo Magda

    2010-06-01

    Full Text Available Se evaluó el efecto de la aplicación de vinazas en algunas propiedades físicas y en la actividad de las deshidrogenasas en un Entic dystropept y en un Fluventic haplustoll del Valle de Cauca, Colombia. Para suplir los requerimientos de K+ del cultivo de maíz dulce (Zea mays L. se utilizaron seis tratamientos (T1 = 100% de K+ como vinaza, T2 = 100% de K+ con KCl; T3 = 50% de K+ como vinaza y KCl, T4 = 75% de K+ con vinaza y 25% de K+ como KCl, T5 = testigo absoluto sin planta, T6 = testigo más planta que fueron dispuestos en un diseño completamente al azar con cinco repeticiones. En el Entic dystropept la densidad aparente disminuyó y en consecuencia la porosidad total aumentó, mientras que en el Fluventic haplustoll sucedió lo contrario. La actividad de las deshidrogenasas fue significativamente mayor (P < 0.05 en el Entic dystropept que en el Fluventic haplustoll que presentó valores bajos. Los resultados mostraron que los cambios que se generan como producto de la aplicación de vinazas son diferentes para ambos tipo de suelo.

  15. Idd13 is involved in determining immunoregulatory DN T-cell number in NOD mice.

    Science.gov (United States)

    Dugas, V; Liston, A; Hillhouse, E E; Collin, R; Chabot-Roy, G; Pelletier, A-N; Beauchamp, C; Hardy, K; Lesage, S

    2014-03-01

    Immunoregulatory T cells have been identified as key modulators of peripheral tolerance and participate in preventing autoimmune diseases. CD4(-)CD8(-) (double negative, DN) T cells compose one of these immunoregulatory T-cell subsets, where the injection of DN T cells confers protection from autoimmune diabetes progression. Interestingly, genetic loci defining the function and number of CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) coincide with at least some autoimmune disease susceptibility loci. Herein, we investigate the impact of major insulin-dependent diabetes (Idd) loci in defining the number of DN T cells. We demonstrate that although Idd3, Idd5 and Idd9 loci do not regulate DN T-cell number, NOD mice congenic for diabetes resistance alleles at the Idd13 locus show a partial restoration in DN T-cell number. Moreover, competitive and non-competitive bone marrow chimera experiments reveal that DN T-cell number is defined by a bone marrow-intrinsic, but DN T-cell-extrinsic, factor. This suggests that non-autonomous candidate genes define DN T-cell number in secondary lymphoid organs. Together, our results show that the regulation of DN T-cell number in NOD mice is at least partially conferred by alleles at the Idd13 locus.

  16. Monovalency Unleashes the Full Therapeutic Potential of the DN-30 Anti-Met Antibody*

    Science.gov (United States)

    Pacchiana, Giovanni; Chiriaco, Cristina; Stella, Maria C.; Petronzelli, Fiorella; De Santis, Rita; Galluzzo, Maria; Carminati, Paolo; Comoglio, Paolo M.; Michieli, Paolo; Vigna, Elisa

    2010-01-01

    Met, the high affinity receptor for hepatocyte growth factor, is one of the most frequently activated tyrosine kinases in human cancer and a validated target for cancer therapy. We previously developed a mouse monoclonal antibody directed against the extracellular portion of Met (DN-30) that induces Met proteolytic cleavage (receptor “shedding”) followed by proteasome-mediated receptor degradation. This translates into inhibition of hepatocyte growth factor/Met-mediated biological activities. However, DN-30 binding to Met also results in partial activation of the Met kinase due to antibody-mediated receptor homodimerization. To safely harness the therapeutic potential of DN-30, its shedding activity must be disassociated from its agonistic activity. Here we show that the DN-30 Fab fragment maintains high affinity Met binding, elicits efficient receptor shedding and down-regulation, and does not promote kinase activation. In Met-addicted tumor cell lines, DN-30 Fab displays potent cytostatic and cytotoxic activity in a dose-dependent fashion. DN-30 Fab also inhibits anchorage-independent growth of several tumor cell lines. In mouse tumorigenesis assays using Met-addicted carcinoma cells, intratumor administration of DN-30 Fab or systemic delivery of a chemically stabilized form of the same molecule results in reduction of Met phosphorylation and inhibition of tumor growth. These data provide proof of concept that monovalency unleashes the full therapeutic potential of the DN-30 antibody and point at DN-30 Fab as a promising tool for Met-targeted therapy. PMID:20833723

  17. Monovalency unleashes the full therapeutic potential of the DN-30 anti-Met antibody.

    Science.gov (United States)

    Pacchiana, Giovanni; Chiriaco, Cristina; Stella, Maria C; Petronzelli, Fiorella; De Santis, Rita; Galluzzo, Maria; Carminati, Paolo; Comoglio, Paolo M; Michieli, Paolo; Vigna, Elisa

    2010-11-12

    Met, the high affinity receptor for hepatocyte growth factor, is one of the most frequently activated tyrosine kinases in human cancer and a validated target for cancer therapy. We previously developed a mouse monoclonal antibody directed against the extracellular portion of Met (DN-30) that induces Met proteolytic cleavage (receptor "shedding") followed by proteasome-mediated receptor degradation. This translates into inhibition of hepatocyte growth factor/Met-mediated biological activities. However, DN-30 binding to Met also results in partial activation of the Met kinase due to antibody-mediated receptor homodimerization. To safely harness the therapeutic potential of DN-30, its shedding activity must be disassociated from its agonistic activity. Here we show that the DN-30 Fab fragment maintains high affinity Met binding, elicits efficient receptor shedding and down-regulation, and does not promote kinase activation. In Met-addicted tumor cell lines, DN-30 Fab displays potent cytostatic and cytotoxic activity in a dose-dependent fashion. DN-30 Fab also inhibits anchorage-independent growth of several tumor cell lines. In mouse tumorigenesis assays using Met-addicted carcinoma cells, intratumor administration of DN-30 Fab or systemic delivery of a chemically stabilized form of the same molecule results in reduction of Met phosphorylation and inhibition of tumor growth. These data provide proof of concept that monovalency unleashes the full therapeutic potential of the DN-30 antibody and point at DN-30 Fab as a promising tool for Met-targeted therapy.

  18. Double negative (DN) αβ T cells: misperception and overdue recognition.

    Science.gov (United States)

    Martina, Maria N; Noel, Sanjeev; Saxena, Ankit; Rabb, Hamid; Hamad, Abdel Rahim A

    2015-03-01

    CD4(-)CD8(-)double negative (DN) αβ T cells are legitimate components of the normal immune system. However, they are poorly understood and largely ignored by immunologists because of their historical association with the lymphoproliferation that occurs in mice (lpr and gld) and humans (autoimmune lymphoproliferative syndromes patients) with impaired Fas-mediated apoptosis where they are considered abnormal T cells. We believe that the traditional view that DN T cells that cause lymphoproliferation (hereafter referred to as lpr DN T cells) are CD4 and CD8 T cells that lost their coreceptor, conceived more than two decades ago, is flawed and that conflating lpr DN T cells with DN T cells found in normal immune system (hereafter referred to as nDN T cells) is unnecessarily dampening interest of this potentially important cell type. To begin rectifying these misperceptions, we will revisit the traditional view of lpr DN T cells and show that it does not hold true in light of recent immunological advances. In lieu of it, we offer a new model proposing that Fas-mediated apoptosis actively removes normally existing DN T cells from the periphery and that impaired Fas-mediated apoptosis leads to accumulation of these cells rather than de novo generation of DN T cells from activated CD4 or CD8 T cells. By doing so, we hope to provoke a new discussion that may lead to a consensus about the origin of lpr DN T cells and regulation of their homeostasis by the Fas pathway and reignite wider interest in nDN T cells.

  19. Proteína C reactiva y lactato deshidrogenasa en el diagnóstico de la obstrucción intestinal en un servicio de urgencias

    Directory of Open Access Journals (Sweden)

    R. Calvo-Rodríguez

    Full Text Available Fundamento. La obstrucción intestinal es una de las urgencias quirúrgicas más frecuentes. Su diagnóstico se basa esencialmente en la historia clínica, la exploración física y las pruebas de imagen. El objetivo de este estudio fue analizar el valor diagnóstico de reactantes de fase aguda en en la obstrucción intestinal y discernir su naturaleza benigna versus maligna. Método. Se ha realizado un estudio de cohortes histórico sobre 53 pacientes sometidos a cirugía por obstrucción intestinal y/o cáncer colorrectal no obstructivo. Los pacientes se encuadraron en 3 grupos: grupo 1 (cáncer colorrectal con obstrucción intestinal (n=23, grupo 2 (obstrucción intestinal benigna (n=10 y grupo 3 (cáncer de colon no obstructivo (n= 20. Se determinan los valores plasmáticos iniciales de la proteína C reactiva (PCR y el enzima lactato deshidrogenasa (LDH. Resultados. La PCR se elevó cuantitativamente más en pacientes con obstrucción intestinal benigna (grupo 2 (p=0,001, mientras que la LDH en el grupo 1 (pacientes con cáncer obstructivo. Los niveles plasmáticos de LDH fueron significativamente mayores en los grupos con obstrucción intestinal (Grupos 1 y 2 que en pacientes sin obstrucción (p 24 ng/l (IC 95% = 30-82% y de 52% (IC 95% = 29-74% para niveles de LDH > 359 U/L. Conclusión. La determinación de las concentraciones plasmáticas de la PCR y la LDH pueden ayudar al diagnóstico de la obstrucción intestinal y orientar hacia su origen benigno o maligno en los servicios de urgencias.

  20. Deficiencia de glucosa 6 fosfato deshidrogenasa: análisis enzimático y molecular en una población de Bogotá

    Directory of Open Access Journals (Sweden)

    Magda Carolina Sánchez

    2008-06-01

    Full Text Available bjetivo: Determinar qué tan frecuente es la deficiencia de glucosa 6 fosfato deshidrogenasa (G6PD y realizar análisis molecular para identificar las variantes A+, A- y mediterránea en una población de residentes en Bogotá.Métodos: Se analizaron 348 personas que residen en Bogotá, pertenecientes a la Policía Nacional y a la Universidad del Rosario. La actividad enzimática se determinó en muestras de sangre mediante espectrofotometría con el kit Trinity Biotech (Cat 345-B. Los valores de hemoglobina (Hg y hematócrito (Hto se determinaron con el método de Drabkin y sedimentación, respectivamente. La determinación de las variantes moleculares se realizó mediante amplificación por reacción en cadena de la polimerasa (PCR y análisis de fragmentos de restricción de longitud polimórfica (RFLP con las enzimas NlaIII, Fok I y MboII para A+, A- y mediterránea, respectivamente. Se hicieron análisis estadísticos para comparar la concentración de Hg en personas sanas y deficientes, la actividad de G6PD por géneros y los datos de frecuencia a nivel mundial.Resultados y conclusiones: La frecuencia de deficiencia de G6PD para la población en estudio fue 3.1%. En 1.4% de los casos se observó actividad deficiente, en 1.7% actividad intermedia y en 0.6% actividad aumentada. No se encontraron las variantes moleculares A+, A- y mediterránea en ningún afectado. La actividad de G6PD no tuvo diferencias por género. Se encontró diferencia significativa en el valor de hemoglobina entre las personas sanas y deficientes de G6PD. Los individuos deficientes eran asintomáticos lo que indica mecanismos de compensación de estrés oxidativo. Las mujeres deficientes son heterocigotos con una inactivación preferencial del cromosoma X anormal y al ser portadoras tienen riesgo de 50% de tener hijos afectados con la enfermedad. La identificación de mujeres y hombres deficientes permite establecer medidas preventivas ante posibles crisis hemol

  1. Mechanism of FADD-DN-Induced Apoptosis in Normal Breast Cells

    Science.gov (United States)

    2005-04-01

    the objectives described in the approved statement of work for years one and two. From 17 FADD-DN interacting proteins identified in a yeast two...that our approach for finding Page 4 mutation in FADD interacting proteins that could restore binding to a mutant FADD-DN would not work. To determine

  2. Developmental dynamics of post-selection thymic DN iNKT.

    Science.gov (United States)

    Yassai, Maryam; Cooley, Brian; Gorski, Jack

    2012-01-01

    Invariant natural killer T (iNKT) cells develop in the thymus and branch off from the maturation pathway of conventional T cell at the DP stage. While different stages of iNKT cellular development have been defined, the actual time that iNKT cell precursors spend at each stage is still unknown. Here we report on maturation dynamics of post-selection DN iNKT cells by injecting wild-type DP(dim) thymocytes into the thymus of TCRα(-/-) mice and using the Vα14-Jα18 rearrangements as a molecular marker to follow the maturation dynamics of these cells. This study shows that the developmental dynamics of DN iNKT cells in DP(dim) are very rapid and that it takes less than 1 day to down-regulate CD4 and CD8 and become DN. These DN cells are precursors of peripheral DN iNKT cells and appear in the spleen in 1-2 days. Thymic DN iNKT residents are predominantly derived from cells that quickly return from the periphery. The expansion of a very small subset of DN iNKT precursors could also play a small role in this process. These data are an example of measuring T cell maturation in the thymus and show that the maturation dynamics of selected DN iNKT cells fall within the same general time frame as conventional αβ T cells.

  3. Dysfunctional hippocampal inhibition in the Ts65Dn mouse model of Down syndrome.

    Science.gov (United States)

    Best, Tyler K; Cramer, Nathan P; Chakrabarti, Lina; Haydar, Tarik F; Galdzicki, Zygmunt

    2012-02-01

    GABAergic dysfunction is implicated in hippocampal deficits of the Ts65Dn mouse model of Down syndrome (DS). Since Ts65Dn mice overexpress G-protein coupled inward-rectifying potassium (GIRK2) containing channels, we sought to evaluate whether increased GABAergic function disrupts the functioning of hippocampal circuitry. After confirming that GABA(B)/GIRK current density is significantly elevated in Ts65Dn CA1 pyramidal neurons, we compared monosynaptic inhibitory inputs in CA1 pyramidal neurons in response to proximal (stratum radiatum; SR) and distal (stratum lacunosum moleculare; SLM) stimulation of diploid and Ts65Dn acute hippocampal slices. Synaptic GABA(B) and GABA(A) mediated currents evoked by SR stimulation were generally unaffected in Ts65Dn CA1 neurons. However, the GABA(B)/GABA(A) ratios evoked by stimulation within the SLM of Ts65Dn hippocampus were significantly larger in magnitude, consistent with increased GABA(B)/GIRK currents after SLM stimulation. These results indicate that GIRK overexpression in Ts65Dn has functional consequences which affect the balance between GABA(B) and GABA(A) inhibition of CA1 pyramidal neurons, most likely in a pathway specific manner, and may contribute to cognitive deficits reported in these mice.

  4. Phase I study of weekly DN-101, a new formulation of calcitriol, in patients with cancer.

    Science.gov (United States)

    Beer, Tomasz M; Javle, Milind M; Ryan, Christopher W; Garzotto, Mark; Lam, Gilbert N; Wong, Alvin; Henner, W David; Johnson, Candace S; Trump, Donald L

    2007-04-01

    DN-101 is a new, high-dose, oral formulation of calcitriol under investigation for the treatment of cancer. We sought to evaluate the tolerability and pharmacokinetics (PK) of weekly doses of DN-101 in patients with advanced cancer. Patients who completed a previously reported single dose escalation study of DN-101 [Beer et al. (2005) Clin Cancer Res 11:7794-7799] were eligible for this continuation weekly dosing study. Cohorts of 3-10 patients were treated at doses of 15, 30, 45, 60, and 75 microg calcitriol. Once 45 microg was established as the maximum tolerated dose (MTD), this cohort was expanded to include 18 patients. Dose limiting toxicity (DLT) was defined as > or =grade 2 hypercalcemia or > or =grade 3 persistent treatment-related toxicities. Thirty-seven patients were recruited. DLT of transient reversible grade 2 hypercalcemia (serum calcium of 11.6-12.5 mg/dL) occurred in two of six patients treated with 60 microg of DN-101. No DLT was observed in the 18 patients who received DN-101 weekly at 45 microg. Overall, DN-101 was well tolerated. The most frequent adverse events were fatigue (27%), hypercalcemia (19%, including five grade 1, two grade 2, and no grade 3 or 4 events), and grade 1 nausea (16%). PK parameters following repeat dosing were comparable to those for the initial dose (n = 4). The MTD for weekly DN-101 was established as 45 mug. The DLTs observed were two episodes of rapidly reversible grade 2 hypercalcemia in two of the six patients treated at 60 microg weekly. Repeat doses of DN-101 at 45 microg weekly are well tolerated and this dose is suitable for studies of weekly DN-101 in cancer patients.

  5. Increased male reproductive success in Ts65Dn “Down syndrome” mice

    OpenAIRE

    Moore, Clara S.; Hawkins, Charles; Franca, Arianna; Lawler, Ann; Devenney, Benjamin; Das, Ishita; Reeves, Roger H.

    2010-01-01

    The Ts65Dn mouse is trisomic for orthologs of about half the genes on Hsa21. A number of phenotypes in these trisomic mice parallel those in humans with trisomy 21 (Down syndrome), including cognitive deficits due to hippocampal malfunction that are sufficiently similar to human that “therapies” developed in Ts65Dn mice are making their way to human clinical trials. However, the impact of the model is limited by availability. Ts65Dn cannot be completely inbred and males are generally consider...

  6. Influence of overexpression of SOCS2 on cells of DN rat.

    Science.gov (United States)

    Bao, Na-Na; Kong, De-Yang; Zhu, Dan; Hao, Li-Rong

    2015-07-01

    To explore the influence and mechanism of overexpression of SOCS2 on diabetic nephropathy (DN) rats and cells. STZ was used to induce male SD rats and SOCS2 was injected into left renal vein. Rats were divided into DN group, DN-Ad-null group and DN-Ad-SOCS2 group. Glucose with high and normal concentration was used to culture HBZY-1 cells and then transfect Ad-SOCS2. HG group, HG-Ad-null group, HG-Ad-SOCS2 group, CG group, CG-Ad-null group, and CG-Ad-SOCS2 group were created. The expression of inflammatory cytokines (MCP-1, TNF-α and IL-6) in kidney tissue of rats, fibrosis related protein (FN, Collagen IV and TGF-β) in kidney tissue and cells of rats, and JAK/STAT signaling pathway related proteins (p-JAK2 and p-STAT3) were tested by western blot. ELISA was used to test the expression of inflammatory cytokines (TNF-α and IL-6) in cells. The expression of inflammatory cytokines in DN rats (MCP-1, TNF-α and IL-6) and cell (TNF-α and IL-6) were increased (P DN animal models and cell models (P DN rats and cells increased while SOCS2 decreased the overexpression of mediated fibrosis related protein in DN model rats and cells (P DN rats and cells increased and the JAK/STAT signaling pathway was activated. Yet, SOCS2 obviously suppressed the expression of the JAK/STAT signaling pathway as well as the related proteins (p-JAK2 and p-STAT3) in both DN rats and cells. The overexpression of SOCS2 can decrease the expression of inflammatory cytokines and fibrosis related proteins in DN rats and cells, and meanwhile suppress the activation of JAK/STAT signaling pathway mediated by DN. Copyright © 2015 Hainan Medical College. Production and hosting by Elsevier B.V. All rights reserved.

  7. A role for E2-2 at the DN3 stage of early thymopoiesis

    DEFF Research Database (Denmark)

    Wikström, Ingela; Forssell, Johan; Penha-Goncalves, Mario N;

    2008-01-01

    proteins, Id2 displayed a prominent expression exclusively in DN1, whereas Id3 showed some expression in DN1, followed by a down regulation and then a prominent induction, peaking in the DP stage. E2-2 was expressed during the DN stages, as well as in the DP stage, suggesting that E2-2 operates in concert...... with the other E-proteins during early thymocyte development. We found that E2-2 null thymocytes displayed a partial block at the DN3 stage of development, as well as a reduced expression of pre-T alpha, known to be regulated also by E2A and HEB. The fact that E2-2 deficient thymocytes develop without gross...

  8. Validation of a New Arabic Version of the Neuropathic Pain Diagnostic Questionnaire (DN4).

    Science.gov (United States)

    Chatila, Nadwa; Pereira, Bruno; Maarrawi, Joseph; Dallel, Radhouane

    2017-01-01

    The "Douleur Neuropathique 4 (DN4) questionnaire" was developed for screening neuropathic pain. The purpose of this work was to validate the DN4 questionnaire in the standard Arabic language. First, the questionnaire was translated and semantically adapted to Arabic according to the international guidelines for cross-cultural adaptation. Second, a prospective observational study was performed to validate this questionnaire. A total of 195 patients with chronic pain (n = 99 with neuropathic pain and n = 96 without neuropathic pain) were enrolled in the study. The internal consistency Kuder-Richardson's Formula 20 for the whole DN4 questionnaire was 0.86 (P pain items. The sensitivity and specificity of the 7-item DN4 and 10-item DN4 were not influenced by either pain severity or educational level. In conclusion, this new Arabic version DN4 questionnaire is a simple, reliable, and valid tool for discriminating between neuropathic and non-neuropathic pain. It represents a useful tool in clinical setting and population-based studies. © 2016 World Institute of Pain.

  9. The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice.

    Science.gov (United States)

    Duan, Suyan; Wu, Yingyi; Zhao, Chuanyan; Chen, Mingyu; Yuan, Yanggang; Xing, Changying; Zhang, Bo

    2016-01-01

    The present study aimed to investigate the relationship between wnt/β-catenin signaling pathway and kidney impairment in diabetic nephropathy (DN) mice as well as the renoprotective effect of rhein (RH). Mice were randomly divided into four groups (n = 6): db/db mice treated with RH (DN + RH), db/db mice (DN), db/m mice treated with RH (NC + RH) and db/m mice (NC). RH-treated groups were administered orally at a daily dose 120 mg/kg. Mice were sacrificed after 12 weeks of treatments. In our study, increased albuminuria, together with weight gain and hyperglycemia was observed in the beginning of the study and continued to increase throughout the length of the study (12 weeks). Histopathologic changes were observed in the DN group. Expectedly, mice receiving the treatment with RH were protected from this injury. Meanwhile, the expression of nephrin, a podocyte-specific marker, was significantly reduced while wnt1, p-GSK-3β/tGSK-3β, p-β-catenin/tβ-catenin were higher in the DN group mice when analyzed by immunofluorescence and Western blotting. RH reversed these above changes. wnt/β-catenin signaling pathway participates in RH ameliorating kidney injury in DN mice. The manipulation of RH might act as a promising therapeutic intervention for DN.

  10. Inhibition of cell growth and telomerase activity in osteosarcoma cells by DN-hTERT.

    Science.gov (United States)

    Xu, Tao; Rao, Yaojian; Zhu, Wentao; Guo, Fengjin

    2006-01-01

    In order to study the effects of dominant negative human telomerase reverse transcriptase (DN-hTERT) on cell growth and telomerase activity in osteosarcoma cell line MG63, MG63 cells were transfected with DN-hTERT-IRES2-EGFP9 (DN) or IRES2-EGF (I, blank vector) with lipofectamine 2000. The stably transfected cells were selected with G-418. Cell growth properties were examined under a fluorescence microscope. The hTERT mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR). Telomerase activities were measured by TRAP-ELISE. The tumorigenicity was studied with tumor xenografts by subcutaneous injection of cancer cells into nude mice. The results showed that cell growth was suppressed in MG63 cells transfected with DN-hTERT. The hTERT mRNA was increased in N-hTERT transfected-MG63 cells (MG63/DN). The telomerase activity was 2.45-0.11 in MG63/DN cells, while 3.40+/-0.12 in the cells transfected with blank vector (MG63/I), (PDN-hTERT-expressing clones did not form tumors in 2 weeks, but the ratio of tumorigenesis was 30 % in nude mice bearing MG63/I (PDN-hTERT could specifically inhibit the cell growth and telomerase activity in MG63 cells.

  11. Determinación de frecuencia cardiaca, frecuencia respiratoria, lactato deshidrogenasa, creatinkinasa y ácido láctico en caballos durante competencia de salto en la Sabana de Bogotá

    Directory of Open Access Journals (Sweden)

    Paula Andrea Guerrero Nieto

    2009-06-01

    Full Text Available El estudio presenta los primeros resultados de investigación en variables fisiológicas y sus cambios por el ejercicio en equinos en competencia de salto en el país. Se utilizaron 24 ejemplares de las razas Silla Argentina, PSI y mestizo de la Escuela de Equitación del Ejército Nacional de Colombia, que participaron en pruebas de salto (1,10 – 1,20 m en diferentes centros ecuestres de la Sabana de Bogotá. Se determinó frecuencia cardiaca, frecuencia respiratoria y enzimas musculares (creatinquinasa (CK, lactato deshidrogenasa (LDH y el ácido láctico.. Se tomaron tres muestras: reposo, inmediatamente después del ejercicio y a las 6 horas posejercicio. Para la frecuencia cardiaca se utilizó un monitor POLAR S625; la frecuencia respiratoria se determinó mediante fonendoscopio; y las enzimas y el ácido láctico se analizaron en laboratorio. Se utilizó el método estadístico ANAVA para enzimas musculares y acido láctico y estadística descriptiva para frecuencia cardiaca y respiratoria. En los resultados se encontró un comportamiento estadísticamente significativo (p < 0.05 del ácido láctico y la enzima creatinkinasa, a diferencia del comportamiento de la enzima lactato deshidrogenasa, que fue no significativo. Las frecuencias cardiaca y respiratoria se elevaron de manera significativa posejercicio y regresaron a lo normal alrededor de las 6 seis horas. El ácido láctico se incrementó posejercicio y disminuyó a las 6 horas, contrario a lo reportado por otros autores. Se concluye que una vez analizadas estas variables, hay diferencias con los valores obtenidos por otros autores, que pueden deberse a la altitud, al tipo y tiempo de ejercicio realizado. Por tanto es indispensable continuar haciendo estudios en este campo.

  12. Dysfunction of the ubiquitin-proteasome system in the cerebellum of aging Ts65Dn mice.

    Science.gov (United States)

    Necchi, Daniela; Lomoio, Selene; Scherini, Elda

    2011-12-01

    In the cerebellum of adult-aging Ts65Dn mice, a murine model of Down syndrome, Purkinje cells undergo degeneration. Searching for the cause of Purkinje cell degeneration, we have studied the ubiquitin-proteasome system (UPS) in the cerebellum of aging Ts65Dn mice. Inhibition of UPS is sufficient to induce neuron degeneration and death. Proteasome chymotrypsin-like proteolytic activity was reduced by 35% in the cerebellum of Ts65Dn mice in comparison with euploid animals. Accordingly, Western blot analysis of ubiquitin showed an increase in ubiquitinated proteins. Immunocytochemistry for ubiquitin revealed strongly positive intranuclear inclusions in Purkinje cells and large neurons of cerebellar nuclei. The Western blot analysis of ubiquitin in nuclear protein extracts confirmed the increase of ubiquitinated proteins in the cell nuclei. After FUS immunocytochemistry, large intranuclear inclusions were visible in Purkinje cells and large neurons of cerebellar nuclei in Ts65Dn mice. Together, data indicate a possible role for proteasome inhibition in the cerebellar neurodegeneration in Ts65Dn mice.

  13. Endurance and failure characteristic of main-shaft jet engine bearing at 3 million DN

    Science.gov (United States)

    Bamberger, E. N.; Zaretsky, E. V.; Signer, H.

    1976-01-01

    Groups of thirty 120-mm bore angular-contact ball bearings were endurance tested at a speed of 12,000 and 25,000 rpm (1.44 million and 3.0 million DN, where DN is the product of the bearing bore in mm and the shaft speed in rpm) and a thrust load of 66,721 N. The bearings were manufactured from a single heat of VIM-VAR AISI M-50 steel. At 1.44 million and 3.0 million DN, 84,483 and 74,800 bearing test hours were accumulated, respectively. Test results were compared with similar bearings made from CVM AISI M-50 steel run under the same conditions. Bearing lives at speeds of 3 million DN with the VIM-VAR AISI M-50 steel were nearly equivalent to those obtained at lower speeds. A combined processing and material life factor of 44 was found for VIM-VAR AISI M-50 steel. Continuous running after a spall has occurred at 3.0 million DN can result in a destructive fracture of the bearing inner race.

  14. Ts65Dn, a mouse model of Down syndrome, exhibits increased GABAB-induced potassium current.

    Science.gov (United States)

    Best, Tyler K; Siarey, Richard J; Galdzicki, Zygmunt

    2007-01-01

    Down syndrome (DS) is the most common nonheritable cause of mental retardation. DS is the result of the presence of an extra chromosome 21 and its phenotype may be a consequence of overexpressed genes from that chromosome. One such gene is Kcnj6/Girk2, which encodes the G-protein-coupled inward rectifying potassium channel subunit 2 (GIRK2). We have recently shown that the DS mouse model, Ts65Dn, overexpresses GIRK2 throughout the brain and in particular the hippocampus. Here we report that this overexpression leads to a significant increase ( approximately 2-fold) in GABA(B)-mediated GIRK current in primary cultured hippocampal neurons. The dose response curves for peak and steady-state GIRK current density is significantly shifted left toward lower concentrations of baclofen in Ts65Dn neurons compared with diploid controls, consistent with increased functional expression of GIRK channels. Stationary fluctuation analysis of baclofen-induced GIRK current from Ts65Dn neurons indicated no significant change in single-channel conductance compared with diploid. However, significant increases in GIRK channel density was found in Ts65Dn neurons. In normalized baclofen-induced GIRK current and GIRK current kinetics no difference was found between diploid and Ts65Dn neurons, which suggests unimpaired mechanisms of interaction between GIRK channel and GABA(B) receptor. These results indicate that increased expression of GIRK2 containing channels have functional consequences that likely affect the balance between excitatory and inhibitory neuronal transmission.

  15. Norfolk QOL-DN: validation of a patient reported outcome measure in transthyretin familial amyloid polyneuropathy.

    Science.gov (United States)

    Vinik, Etta J; Vinik, Aaron I; Paulson, James F; Merkies, Ingemar S J; Packman, Jeff; Grogan, Donna R; Coelho, Teresa

    2014-06-01

    The Norfolk Quality of Life-Diabetic Neuropathy (QOL-DN) questionnaire is an instrument to assess QOL in diabetic polyneuropathy. The objective of this observational, cross-sectional study in 61 patients with V30M transthyretin familial amyloid polyneuropathy (TTR-FAP) and 16 healthy volunteers was to validate the Norfolk QOL-DN for assessment of QOL in TTR-FAP. Comparisons were conducted to identify the best items to discriminate disease stages and assess which individual Norfolk domains (symptoms, large fiber, small fiber, autonomic, and activities of daily living) would be most affected by disease stage. Analysis of individual items revealed a significant pattern of discrimination among disease stages (p DN is a reliable indicator of the impact of disease severity on QOL in patients with TTR-FAP. © 2014 Peripheral Nerve Society.

  16. Digastric Muscle Phenotypes of the Ts65Dn Mouse Model of Down Syndrome.

    Directory of Open Access Journals (Sweden)

    Tiffany J Glass

    Full Text Available Down syndrome is frequently associated with complex difficulties in oromotor development, feeding, and swallowing. However, the muscle phenotypes underlying these deficits are unclear. We tested the hypotheses that the Ts65Dn mouse model of DS has significantly altered myosin heavy chain (MyHC isoform profiles of the muscles involved in feeding and swallowing, as well as reductions in the speed of these movements during behavioral assays. SDS-PAGE, immunofluorescence, and qRT-PCR were used to assess MyHC isoform expression in pertinent muscles, and functional feeding and swallowing performance were quantified through videofluoroscopy and mastication assays. We found that both the anterior digastric (ADG and posterior digastric (PDG muscles in 11-day old and 5-6 week old Ts65Dn groups showed significantly lower MyHC 2b protein levels than in age-matched euploid control groups. In videofluoroscopic and videotape assays used to quantify swallowing and mastication performance, 5-6 week old Ts65Dn and euploid controls showed similar swallow rates, inter-swallow intervals, and mastication rates. In analysis of adults, 10-11 week old Ts65Dn mice revealed significantly less MyHC 2b mRNA expression in the posterior digastric, but not the anterior digastric muscle as compared with euploid controls. Analysis of MyHC 2b protein levels across an adult age range (10-53 weeks of age revealed lower levels of MyHC 2b protein in the PDG of Ts65Dn than in euploids, but similar levels of MyHC 2b in the ADG. Cumulatively, these results indicate biochemical differences in some, but not all, muscles involved in swallowing and jaw movement in Ts65Dn mice that manifest early in post-natal development, and persist into adulthood. These findings suggest potential utility of this model for future investigations of the mechanisms of oromotor difficulties associated with Down syndrome.

  17. Glucosa-6-fosfato deshidrogenasa (G6PD. Respuesta de los hematíes y otras células humanas a la disminución en su actividad

    Directory of Open Access Journals (Sweden)

    Javier Fernando Bonilla

    2007-03-01

    Full Text Available La glucosa-6-fosfato deshidrogenasa (G6PD es la primera enzima de la vía pentosa fosfato y la principal fuente intracelular de nicotidamina adenina dinucleótido fosfato reducido (NADPH, compuesto comprometido en diversos procesos fisiológicos, por ejemplo defensa antioxidante (sobre todo células como los eritrocitos, modulación del crecimiento endotelial, eritropoyesis, vascularización y fagocitosis. La deficiencia de G6PD es la enzimopatía ligada al cromosoma X más común en el ser humano. Si bien se puede presentar en cualquier tipo de célula, su carencia absoluta es incompatible con la vida. Según la OMS, en el mundo hay más de 400 millones de personas afectadas por la deficiencia de la enzima, y para Colombia calculan una prevalencia de la deficiencia severa entre 3% y 7%, pero no se conocen los datos relativos a las alteraciones leves y moderadas, que también tienen efectos clínicos. El presente artículo revisa los aspectos biomoleculares más importantes de la enzima, su clasificación de acuerdo con la actividad y la movilidad electroforética, y también se mencionan algunos aspectos clínicos relacionados con la alteración de su actividad.

  18. Laparoendoscopic single-site donor nephrectomy (LESS-DN) versus standard laparoscopic donor nephrectomy.

    Science.gov (United States)

    Gupta, Ameet; Ahmed, Kamran; Kynaston, Howard G; Dasgupta, Prokar; Chlosta, Piotr L; Aboumarzouk, Omar M

    2016-05-27

    Advances in minimally invasive surgery for live kidney donors have led to the development of laparoendoscopic single site donor nephrectomy (LESS-DN). At present, laparoscopic donor nephrectomy is the technique of choice for donor nephrectomy globally. Compared with open surgical approaches, laparoscopic donor nephrectomy is associated with decreased morbidity, faster recovery times and return to normal activity, and shorter hospital stays. LESS-DN differs from standard laparoscopic donor nephrectomy; LESS-DN requires a single incision through which the procedure is performed and donor kidney is removed. Previous studies have hypothesised that LESS-DN may provide additional benefits for kidney donors and stimulate increased donor rates. This review looked at the benefits and harms of LESS-DN compared with standard laparoscopic nephrectomy for live kidney donors. We searched the Cochrane Kidney and Transplant's Specialised Register to 28 January 2016 through contact with the Information Specialist using search terms relevant to this review. We included randomised controlled trials (RCTs) that compared LESS-DN with laparoscopic donor nephrectomy in adults. Three authors independently assessed studies for eligibility and conducted risk of bias evaluation. Summary estimates of effect were obtained using a random-effects model and results were expressed as risk ratios (RR) or risk difference (RD) and their 95% confidence intervals (CI) for dichotomous outcomes, and mean difference (MD) and 95% CI for continuous outcomes. We included three studies (179 participants) comparing LESS-DN with laparoscopic donor nephrectomy. There were no significant differences between LESS-DN and laparoscopic donor nephrectomy for mean operative time (2 studies, 79 participants: MD 6.36 min, 95% CI -11.85 to 24.57), intra-operative blood loss (2 studies, 79 participants: MD -8.31 mL, 95% CI -23.70 to 7.09), or complication rates (3 studies, 179 participants: RD 0.05, 95% CI -0.04 to 0

  19. Validation of the Greek Version of the DN4 Diagnostic Questionnaire for Neuropathic Pain.

    Science.gov (United States)

    Sykioti, Panagiota; Zis, Panagiotis; Vadalouca, Athina; Siafaka, Ioanna; Argyra, Eriphili; Bouhassira, Didier; Stavropoulou, Evmorfia; Karandreas, Nikolaos

    2015-09-01

    The Douleur Neuropathique 4 questionnaire (DN4) was developed by the French Neuropathic Pain Group and is a simple and objective tool, primarily designed to screen for neuropathic pain. The aim of our study is to validate the DN4 in the Greek language. The study was set up as a prospective observational study. Two pain specialists independently examined patients and diagnosed them with neuropathic, nociceptive, or mixed pain, according to the International Association for the Study of Pain (IASP) definitions. A third and a fourth physician administered the DN4 questionnaire to the patients. Out of the 237 patients who met our inclusion criteria and had identical diagnoses regarding the type of pain, 123 were diagnosed with neuropathic, 59 with nociceptive, and 55 with mixed pain. Among patients with identical diagnoses of neuropathic or nociceptive pain, using a receiver operating characteristic (ROC) curve analysis, the area under the curve (AUC) was 0.92. A cutoff point of equal or greater than 4 resulted in a sensitivity of 93% and a specificity of 78%. Among patients suffering from pain with neuropathic element (neuropathic or mixed pain) or pain with no neuropathic element (nociceptive pain), using a ROC curve analysis, the AUC was 0.89. A cutoff point of equal or greater than 4 resulted in a sensitivity of 89% and a specificity of 78%. The Greek version of DN4 is a valid tool for discriminating between neuropathic and nociceptive pain conditions in daily practice. © 2014 World Institute of Pain.

  20. DnB NORD Grupi majandusanalüüs : kinnisvaral on veel kasvuruumi / Kaire Talviste

    Index Scriptorium Estoniae

    Talviste, Kaire, 1979-

    2007-01-01

    Tallinna, Riia ja Vilniuse kinnisvarahindade kasvu võrdlus. DnB NORD Grupp peab tõenäoliseks hindade stabiliseerumist kõigis Balti riikides. Kui arvestada eluasemelaenude portfelli suhet SKT-sse, siis on kinnisvarahindadel veel kasvuruumi, hinnatõusude võimalus on suurem Leedus ja Lätis. Diagramm, tabel

  1. Axonal abnormalities in cerebellar Purkinje cells of the Ts65Dn mouse.

    Science.gov (United States)

    Necchi, Daniela; Lomoio, Selene; Scherini, Elda

    2008-10-31

    Ts65Dn mice are a genetic model for Down syndrome. Among others, these mice have cerebellar pathology features which parallel those seen in Down syndrome patients. Both individuals with Down syndrome and Ts65Dn mice have reduced cerebellar volume and numbers of granule and Purkinje cells. In this report, we describe morphological abnormalities of axons of Purkinje cells in the cerebellum of Ts65Dn mice, by using anti-calbindin immunocytochemistry. A consistent number of Purkinje cells shows axons bearing giant varicosities along their transit through the granular layer. The cerebellar arbor vitae made by fasciculated Purkinje cell axons has a patchy appearance, some tracks being devoid of calbindin staining. The infraganglionic plexus, formed by recurrent collaterals of Purkinje cell axons, has enormously increased density, which is evidence for a compensatory reaction to degeneration of distal segments of axons. These alterations are accompanied by strong glial reaction as evidenced by GFAP immunocytochemistry. Moreover, the alterations are more consistent in the anterior lobules of the vermis and intermediate cortex. The axonal pathology of Purkinje cells may explain the impairment in cerebellar functions observed in Ts65Dn mice at the adulthood.

  2. Study of the Correlation between the Level of CRP and Chemerin of Serum and the Occurrence and Development of DN.

    Science.gov (United States)

    Wang, Yunqian; Wang, Min; Chen, Baoping; Shi, Jun

    2015-01-01

    The aim of the study was to find the correlation between CRP and chemerin in development of DN. We choose 90 type-2 diabetic patients between February 2010 and February 2013, who were then divided into DN group and healthy control group. The results of BP showed that there is no difference in SBP and DBP of patients in the three groups. HDL-C of patients in diabetic group and DN group is lower compared with control. CRP in diabetic group and DN group is higher than that of patients in control group. Comparing the patients in DN group with that in diabeteic group, CRP was significantly higher. Chemerin level in the diabetic group and DN group is higher than control group. When comparing the patients in DN group with those in diabeteic group, serum level of chemerin was significantly higher. Serum level of chemerin is negatively correlated with HDL-C and positively correlated with FPG, HbA1c, LDL-C, BUN and Scr. Serum CRP is negatively correlated with HDL-C and positively correlated with FPG, HbA1c, LDL-C, BUN and Scr. Serum level of chemerin is positively correlated with CRP (r=0.701, PDN patients rose significantly, and may participate in the occurrence and development of DN.

  3. Incremento de la glucosa-6-fosfato-deshidrogenasa eritrocitaria en jóvenes con síndrome de Down tras un programa de actividad física de 12 semanas A 12-week physical activity program increases glucose-6-phosphate-dehydrogenase activity in Down syndrome adolescents

    OpenAIRE

    Francisco J. Ordóñez; Manuel Rosety-Rodríguez; Manuel Rosety

    2005-01-01

    Recientemente se ha publicado que las células trisómicas presentan una mayor sensibilidad al daño oxidativo, que podría justificar la frecuente asociación de síndrome de Down a aterosclerosis, envejecimiento precoz, etc. Para conocer el posible papel de la actividad física moderada en la mejora de la capacidad antioxidante se estudió el comportamiento de la enzima glucosa-6-fosfato-deshidrogenasa (G6PDH) eritrocitaria en 31 adolescentes varones (16.3 ± 1.1 años) tras desarrollar un programa d...

  4. Predisposición genética en el consumo de alcohol: el caso de la Alcohol Deshidrogenasa 1C Genetic predisposition to alcohol consumption: The case of Alcohol Dehydrogenase 1C

    Directory of Open Access Journals (Sweden)

    F. Francès

    2007-07-01

    Full Text Available Introducción: El consumo de alcohol se presenta frecuentemente asociado a determinados delitos, siendo en unas ocasiones atenuante o eximente, y en otras una infracción penal per se. Se han identificado numerosos factores genéticos y ambientales que predisponen al consumo de alcohol. Nuestro objetivo ha sido estudiar la prevalencia del polimorfismo Ile349Val en la alcohol deshidrogenasa 1C que da lugar a la isoforma gamma 2 (metabolizador lento, y estudiar su asociación con el consumo de alcohol así como reflexionar sobre la dimensión de la implicación de estas variantes genéticas en la Medicina Legal. Material y Métodos: Se ha genotipado el polimorfismo Ile 349Val en 869 individuos procedentes de una población mediterránea española. Se ha estimado su prevalencia y su asociación con el consumo de alcohol tanto de manera contínua como categórica. Resultados: La prevalencia de la variante fue: 41%Ile/Ile, 44,5%Ile/Val y 14%Val/Val. En las mujeres Val/Val (homozigotas para la variante gamma 2, el consumo de alcohol fue superior a las portadoras de la variante gamma 1 (Ile; p=0,013. Además, el riesgo de consumo elevado de alcohol en estas mujeres fue estadísticamente significativo (OR 2,59: IC al 95%: 1,01-6,65; p=0,048. Conclusión: En nuestro estudio, la variante Ile349val en el gen de la Alcohol Deshidrogenasa 1C está asociada con el riesgo de mayor consumo de alcohol en las mujeres. Estos datos hacen pensar en la posibilidad futura de valorar el perfil de genes asociados al consumo de alcohol en personas imputadas en determinados actos en estado de ebriedad, pudiendo matizar potencialmente la voluntariedad e imputabilidad de dicho acto ilícito.Introduction: Alcohol consumption is present in several crimes, being an extenuating or exculpating circumstance. In other cases it represents per se a penal infraction. Several genetic and environmental factors predisposing to alcohol consumption have been identified. Our aim is to

  5. The PI3K/p-Akt signaling pathway participates in calcitriol ameliorating podocyte injury in DN rats.

    Science.gov (United States)

    Song, Zhixia; Guo, Yinfeng; Zhou, Min; Zhang, Xiaoliang

    2014-10-01

    The present study aimed to investigate the relationship between PI3K/p-Akt signaling pathway and podocyte impairment in DN rats as well as the protective effect of calcitriol. SD rats were randomly divided into four groups: normal control (NC), normal treated with calcitriol (NC+VD), diabetic nephropathy (DN) and DN treated with calcitriol (DN+VD); all VD rats were treated with 0.1 μg/kg/d calcitriol by gavage. DN model rats were established by intraperitoneal injections of streptozotocin (STZ). Rats were sacrificed after 18 weeks of treatments. In the present study, increased albuminuria was observed as early as 3 weeks of diabetes and continued to increase more than six-fold throughout the length of the study (18 weeks). Expectedly, animals receiving the treatment with calcitriol was protected from this increase, lower about one third. Meanwhile, the expression of podocyte specific markers, including nephrin and podocin, together with PI3K/p-Akt was significantly decreased in DN rats, whereas calcitriol reversed these above changes accompanied by elevated the expression levels of VDR. Additionally, a positive correlation was observed between the expression levels of nephrin and VDR (r = 0.776, P DN rats. The manipulation of calcitriol might act as a promising therapeutic intervention for diabetic nephropathy. Copyright © 2014 Elsevier Inc. All rights reserved.

  6. A D-N Alternating Algorithm for Solving 3D Exterior Helmholtz Problems

    Directory of Open Access Journals (Sweden)

    Qing Chen

    2014-01-01

    Full Text Available The nonoverlapping domain decomposition method, which is based on the natural boundary reduction, is applied to solve the exterior Helmholtz problem over a three-dimensional domain. The basic idea is to introduce a spherical artificial boundary; the original unbounded domain is changed into a bounded subdomain and a typical unbounded region; then, a Dirichlet-Nuemann (D-N alternating method is presented; the finite element method and natural boundary element methods are alternately applied to solve the problems in the bounded subdomain and the typical unbounded subdomain. The convergence of the D-N alternating algorithm and its discretization are studied. Some numerical experiments are presented to show the performance of this method.

  7. $NDK$, $\\bar{K} DN$ and $ND\\bar{D}$ molecules

    CERN Document Server

    Xiao, C W; Oset, E

    2011-01-01

    We investigate theoretically baryon systems made of three hadrons which contain one nucleon and one D meson, and in addition another meson, $\\bar{D}, K$ or $\\bar{K}$. The systems are studied using the Fixed Center Approximation to the Faddeev equations. The study is made assuming scattering of a $K$ or a $\\bar{K}$ on a $DN$ cluster, which is known to generate the $\\Lambda_c(2595)$, or the scattering of a nucleon on the $D\\bar{D}$ cluster, which has been shown to generate a hidden charm resonance named X(3700). We also investigate the configuration of scattering of $N$ on the $KD$ cluster, which is known to generate the $D_{s0}^*(2317)$. In all cases we find bound states, with the $NDK$ system, of exotic nature, more bound than the $\\bar{K} DN$.

  8. Generation of a Retinoblastoma (Rb)1-inducible dominant-negative (DN) mouse model.

    Science.gov (United States)

    Tarang, Shikha; Doi, Songila M S R; Gurumurthy, Channabasavaiah B; Harms, Donald; Quadros, Rolen; Rocha-Sanchez, Sonia M

    2015-01-01

    Retinoblastoma 1 (Rb1) is an essential gene regulating cellular proliferation, differentiation, and homeostasis. To exert these functions, Rb1 is recruited and physically interacts with a growing variety of signaling pathways. While Rb1 does not appear to be ubiquitously expressed, its expression has been confirmed in a variety of hematopoietic and neuronal-derived cells, including the inner ear hair cells (HCs). Studies in transgenic mice demonstrate that complete germline or conditional Rb1 deletion leads to abnormal cell proliferation, followed by massive apoptosis; making it difficult to fully address Rb1's biochemical activities. To overcome these limitations, we developed a tetracycline-inducible TetO-CB-myc6-Rb1 (CBRb) mouse model to achieve transient and inducible dominant-negative (DN) inhibition of the endogenous RB1 protein. Our strategy involved fusing the Rb1 gene to the lysosomal protease pre-procathepsin B (CB), thus allowing for further routing of the DN-CBRb fusion protein and its interacting complexes for proteolytic degradation. Moreover, reversibility of the system is achieved upon suppression of doxycycline (Dox) administration. Preliminary characterization of DN-CBRb mice bred to a ubiquitous rtTA mouse line demonstrated a significant inhibition of the endogenous RB1 protein in the inner ear and in a number of other organs where RB1 is expressed. Examination of the postnatal (P) DN-CBRb mice inner ear at P10 and P28 showed the presence of supernumerary inner HCs (IHCs) in the lower turns of the cochleae, which corresponds to the described expression domain of the endogenous Rb1 gene. Selective and reversible suppression of gene expression is both an experimental tool for defining function and a potential means to medical therapy. Given the limitations associated with Rb1-null mice lethality, this model provides a valuable resource for understanding RB1 activity, relative contribution to HC regeneration and its potential therapeutic application.

  9. Narcolepsy is a common phenotype in HSAN IE and ADCA-DN.

    Science.gov (United States)

    Moghadam, Keivan Kaveh; Pizza, Fabio; La Morgia, Chiara; Franceschini, Christian; Tonon, Caterina; Lodi, Raffaele; Barboni, Piero; Seri, Marco; Ferrari, Simona; Liguori, Rocco; Donadio, Vincenzo; Parchi, Piero; Cornelio, Ferdinando; Inzitari, Domenico; Mignarri, Andrea; Capocchi, Giuseppe; Dotti, Maria Teresa; Winkelmann, Juliane; Lin, Ling; Mignot, Emmanuel; Carelli, Valerio; Plazzi, Giuseppe

    2014-06-01

    We report on the extensive phenotypic characterization of five Italian patients from four unrelated families carrying dominant heterozygous DNMT1 mutations linked to two distinct autosomal dominant diseases: hereditary sensory and autonomic neuropathy with dementia and hearing loss type IE (HSAN IE) and autosomal dominant cerebellar ataxia, deafness and narcolepsy (ADCA-DN). Patients underwent genetic analysis of DNMT1 gene, neurophysiological tests investigating sleep, auditory functions and peripheral nervous system, ophthalmological studies including optical coherence tomography, lymphoscintigraphy, brain magnetic resonance and nuclear imaging, cerebrospinal fluid hypocretin-1, total tau, phosphorylated tau, amyloid-β1-42 and 14-3-3 proteins measurement, skin, muscular and sural nerve biopsies. Exome and direct sequencing studies disclosed two different point mutations affecting exon 21 of DNMT1 gene in patients with ADCA-DN, a novel heterozygous point mutation in exon 20 in two affected HSAN IE siblings, and a trinucleotide deletion in exon 20 in the latter patient with HSAN IE. Phenotypic characterization pinpoints that ADCA-DN and HSAN IE represent two discrete clinical entities belonging to the same disease spectrum, with variable degree of overlap. Remarkably, narcolepsy with or without cataplexy with low/intermediate or normal cerebrospinal fluid hypocretin-1 is present in both diseases. The human leukocyte antigen DQB1*06:02 was absent in all patients. Other common symptoms and features observed in our cases, involving the central and peripheral nervous system, include deafness, optic neuropathy-previously not reported in HSAN IE-large and small fibres polyneuropathy and lower limbs oedema. Overall, the two syndromes share more characteristics than previously recognized and narcolepsy is common to both. HSAN IE and ADCA-DN are two extreme phenotypic manifestations of a DNMT1 methylopathy. © The Author (2014). Published by Oxford University Press on

  10. Application of electron paramagnetic resonance spectroscopy for validation of the novel (AN+DN) solvent polarity scale.

    Science.gov (United States)

    Malavolta, Luciana; Poletti, Erick F; Silva, Elias H; Schreier, Shirley; Nakaie, Clovis R

    2008-06-01

    Based on solvation studies of polymers, the sum (1:1) of the electron acceptor (AN) and electron donor (DN) values of solvents has been proposed as an alternative polarity scale. To test this, the electron paramagnetic resonance isotropic hyperfine splitting constant, a parameter known to be dependent on the polarity/proticity of the medium, was correlated with the (AN+DN) term using three paramagnetic probes. The linear regression coefficient calculated for 15 different solvents was approximately 0.9, quite similar to those of other well-known polarity parameters, attesting to the validity of the (AN+DN) term as a novel "two-parameter" solvent polarity scale.

  11. Elucidation of fluoranthene degradative characteristics in a newly isolated Achromobacter xylosoxidans DN002.

    Science.gov (United States)

    Ma, Yan-Ling; Lu, Wei; Wan, Li-Li; Luo, Na

    2015-02-01

    Strain DN002 isolated from petroleum-contaminated soil was identified as Achromobacter xylosoxidans based on morphological and biochemical properties and 16S rRNA phylogeny, and investigated for its potential to utilize numerous polycyclic aromatic hydrocarbons (PAHs) such as fluoranthene and pyrene as sole carbon and energy resource. Biodegradation studies showed that 500 mg(·)l(-1)fluranthene was degraded to 35.6 ± 0.3 mg(·)l(-1) by DN002 after 14 days incubation. During fluoranthene biodegradation, catechol 2,3 dioxygenase (C23O) activity was augmented 1.5 times more than catechol 1,2 dioxygenase (C12O), which indicated that C23O played a major role in fluoranthene degradation by DN002. Protein profiles were examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and two-dimensional electrophoresis then analyzed by mass spectrometry induced by fluoranthene; a molecular mass range of 18 ∼ 66 kDa proteins were found upregulated compared with the uninduced control sample, including multiple isoenzymes of β-oxidation and dehydrogenases as well as dioxygenases. Besides, some new proteins, i.e., dihydrolipoamide succinyltransferase and aldehyde dehydrogenase family proteins and isocitrate lyase were also synthesized.

  12. Synaptic Vesicle Recycling Is Unaffected in the Ts65Dn Mouse Model of Down Syndrome.

    Science.gov (United States)

    Marland, Jamie R K; Smillie, Karen J; Cousin, Michael A

    2016-01-01

    Down syndrome (DS) is the most common genetic cause of intellectual disability, and arises from trisomy of human chromosome 21. Accumulating evidence from studies of both DS patient tissue and mouse models has suggested that synaptic dysfunction is a key factor in the disorder. The presence of several genes within the DS trisomy that are either directly or indirectly linked to synaptic vesicle (SV) endocytosis suggested that presynaptic dysfunction could underlie some of these synaptic defects. Therefore we determined whether SV recycling was altered in neurons from the Ts65Dn mouse, the best characterised model of DS to date. We found that SV exocytosis, the size of the SV recycling pool, clathrin-mediated endocytosis, activity-dependent bulk endocytosis and SV generation from bulk endosomes were all unaffected by the presence of the Ts65Dn trisomy. These results were obtained using battery of complementary assays employing genetically-encoded fluorescent reporters of SV cargo trafficking, and fluorescent and morphological assays of fluid-phase uptake in primary neuronal culture. The absence of presynaptic dysfunction in central nerve terminals of the Ts65Dn mouse suggests that future research should focus on the established alterations in excitatory / inhibitory balance as a potential route for future pharmacotherapy.

  13. Synaptic Vesicle Recycling Is Unaffected in the Ts65Dn Mouse Model of Down Syndrome.

    Directory of Open Access Journals (Sweden)

    Jamie R K Marland

    Full Text Available Down syndrome (DS is the most common genetic cause of intellectual disability, and arises from trisomy of human chromosome 21. Accumulating evidence from studies of both DS patient tissue and mouse models has suggested that synaptic dysfunction is a key factor in the disorder. The presence of several genes within the DS trisomy that are either directly or indirectly linked to synaptic vesicle (SV endocytosis suggested that presynaptic dysfunction could underlie some of these synaptic defects. Therefore we determined whether SV recycling was altered in neurons from the Ts65Dn mouse, the best characterised model of DS to date. We found that SV exocytosis, the size of the SV recycling pool, clathrin-mediated endocytosis, activity-dependent bulk endocytosis and SV generation from bulk endosomes were all unaffected by the presence of the Ts65Dn trisomy. These results were obtained using battery of complementary assays employing genetically-encoded fluorescent reporters of SV cargo trafficking, and fluorescent and morphological assays of fluid-phase uptake in primary neuronal culture. The absence of presynaptic dysfunction in central nerve terminals of the Ts65Dn mouse suggests that future research should focus on the established alterations in excitatory / inhibitory balance as a potential route for future pharmacotherapy.

  14. Examining the effect of nonlocality in $(d,n)$ transfer reactions

    CERN Document Server

    Ross, A; Nunes, F M

    2016-01-01

    Background: In the last year we have been exploring the effect of the explicit inclusion of nonlocality in (d,p) reactions. Purpose: The goal of this work is to extend previous studies to (d,n) reactions, which, although similar to (d,p), have specific properties that merit inspection. Method: We apply our methods (both the distorted wave Born approximation and the adiabatic wave approximation) to $(d,n)$ reactions on $^{16}$O, $^{40}$Ca, $^{48}$Ca, $^{126}$Sn, $^{132}$Sn, and $^{208}$Pb at $20$ and $50$ MeV. Results: We look separately at the modifications introduced by nonlocality in the final bound and scattering states, as well as the consequences reflected on the differential angular distributions. The cross sections obtained when using nonlocality explicitly are significantly different than those using the local approximation, just as in (d,p). Due to the particular role of Coulomb in the bound state, often we found the effects of nonlocality to be larger in (d,n) than in (d,p). Conclusions: Our results...

  15. Comparative Analysis of SN 2012dn Optical Spectra: Days -14 to +114

    CERN Document Server

    Parrent, J T; Fesen, R A; Parker, S; Bianco, F B; Dilday, B; Sand, D; Valenti, S; Vinkó, J; Berlind, P; Challis, P; Milisavljevic, D; Sanders, N; Marion, G H; Wheeler, J C; Brown, P; Calkins, M L; Friesen, B; Kirshner, R; Pritchard, T; Quimby, R; Roming, P

    2016-01-01

    SN 2012dn is a super-Chandrasekhar mass candidate in a purportedly normal spiral (SAcd) galaxy, and poses a challenge for theories of type Ia supernova diversity. Here we utilize the fast and highly parameterized spectrum synthesis tool, SYNAPPS, to estimate relative expansion velocities of species inferred from optical spectra obtained with six facilities. As with previous studies of normal SN Ia, we find that both unburned carbon and intermediate mass elements are spatially coincident within the ejecta near and below 14,000 km/s. Although the upper limit on SN 2012dn's peak luminosity is comparable to some of the most luminous normal SN Ia, we find a progenitor mass exceeding ~1.6 Msun is not strongly favored by leading merger models since these models do not accurately predict spectroscopic observations of SN 2012dn and more normal events. In addition, a comparison of light curves and host-galaxy masses for a sample of literature and Palomar Transient Factory SN Ia reveals a diverse distribution of SN Ia s...

  16. Altered distribution of hippocampal interneurons in the murine Down Syndrome model Ts65Dn.

    Science.gov (United States)

    Hernández-González, Samuel; Ballestín, Raúl; López-Hidalgo, Rosa; Gilabert-Juan, Javier; Blasco-Ibáñez, José Miguel; Crespo, Carlos; Nácher, Juan; Varea, Emilio

    2015-01-01

    Down Syndrome, with an incidence of one in 800 live births, is the most common genetic alteration producing intellectual disability. We have used the Ts65Dn model, that mimics some of the alterations observed in Down Syndrome. This genetic alteration induces an imbalance between excitation and inhibition that has been suggested as responsible for the cognitive impairment present in this syndrome. The hippocampus has a crucial role in memory processing and is an important area to analyze this imbalance. In this report we have analysed, in the hippocampus of Ts65Dn mice, the expression of synaptic markers: synaptophysin, vesicular glutamate transporter-1 and isoform 67 of the glutamic acid decarboxylase; and of different subtypes of inhibitory neurons (Calbindin D-28k, parvalbumin, calretinin, NPY, CCK, VIP and somatostatin). We have observed alterations in the inhibitory neuropil in the hippocampus of Ts65Dn mice. There was an excess of inhibitory puncta and a reduction of the excitatory ones. In agreement with this observation, we have observed an increase in the number of inhibitory neurons in CA1 and CA3, mainly interneurons expressing calbindin, calretinin, NPY and VIP, whereas parvalbumin cell numbers were not affected. These alterations in the number of interneurons, but especially the alterations in the proportion of the different types, may influence the normal function of inhibitory circuits and underlie the cognitive deficits observed in DS.

  17. Efecto del pirofosfato de tiamina en la morfología del hepatocito normal, y en el potencial de membrana de células hepáticas, de cerebro y de cerebelo de ratas intoxicadas con CCl4

    OpenAIRE

    Heberto Alcázar-Montenegro; Elsa Aguirre-Benítez; Susana Alcázar-Leyva; Noé Alvarado-Vásquez; María Teresa Benítez-Rodríguez

    2000-01-01

    La coenzima pirofosfato de tiamina (PPT) es importante para el funcionamiento de la piruvato-deshidrogenasa y de la a-cetoglutarato deshidrogenasa que intervienen en el metabolismo celular. En especial, órganos como el corazón, el hígado o el cerebro son dependientes de la presencia del PPT para su actividad. En este estudio, se evaluó al nivel hepático el efecto de la aplicación de dosis suprafisiológicas del PPT en la morfología celular y nuclear de hepatocitos de ratas Wistar macho normale...

  18. A novel heterotrophic nitrifying and aerobic denitrifying bacterium, Zobellella taiwanensis DN-7, can remove high-strength ammonium.

    Science.gov (United States)

    Lei, Yu; Wang, Yangqing; Liu, Hongjie; Xi, Chuanwu; Song, Liyan

    2016-05-01

    A novel heterotrophic bacterium capable of heterotrophic nitrification and aerobic denitrification was isolated from ammonium contaminated landfill leachate and physiochemical and phylogenetically identified as Zobellella taiwanensis DN-7. DN-7 converted nitrate, nitrate, and ammonium to N2 as the primary end product. Single factor experiments suggested that the optimal conditions for ammonium removal were trisodium citrate as carbon source, C/N ratio 8, pH 8.0-10.0, salinity less than 3 %, temperature 30 °C, and rotation speed more than 150 rpm. Specifically, DN-7 could remove 1000.0 and 2000.0 mg/L NH4 (+)-N completely within 96 and 216 h, with maximum removal rates of 19.6 and 17.3 mg L(-1) h(-1), respectively. These results demonstrated that DN-7 is a promising candidate for application of high-strength ammonium wastewater treatments.

  19. [Effect of dominant negative HIF-1alpha (dn HIF-1alpha) on biological characteristics of uterine cervix cancer cells].

    Science.gov (United States)

    Tang, Bin-Zhi; Zhao, Feng-Yan; Wei, Ting; Mu, De-Zhi; Mao, Meng; Fu, Qiang; Zhang, Lin; Qu, Yia

    2008-05-01

    To explore the effect of dominant negative HIF-1alpha (dn HIF-1alpha) on biological characteristics of uterine cervix cancer cell SiHa and elucidate the related mechanism. pcDNA3. 1-dn HIF-1alpha was transfected into SiHa cells. The expression of HIF-1alpha and VEGF protein were detected by immunocytochemical method and Western Blotting. The growth proliferation of cells was surveyed by the MTT assay and cell apoptosis was detected through TUNEL after treated with CoCl2, meanwhile the results were compared with the group transfected with mock plasmid and untransfected group. After successfully transfected with relevant plasmid, there's no obvious difference of expression of HIF-1alpha among dn HIF-1alpha group, pcDNA3. 1 group, and untransfected group, however the expression of VEGF of dn HIF-1alpha group was significantly lower than that of the others (P dn HIF-1alpha group was obviously lower than that of the other two (P dn HIF-1alpha group among these three (P < 0.05). Domain negative HIF-1alpha can inhibit the proliferation of uterine cervix cancer cell and accelerate its apoptosis under hypoxia induced by CoCl2, as well as decrease the expression of VEGF protein. The implications of all this were that the domain negative HIF-1alpha may play an important role in the therapy of uterine cervix cancer.

  20. Recent Progress in the Treatment of Advanced Prostate Cancer With Intermittent Dose-Intense Calcitriol (DN-101).

    Science.gov (United States)

    Brawer, Michael K

    2007-01-01

    Docetaxel is becoming standard therapy for androgen-independent prostate cancer (AIPC), and investigational agents are being added to docetaxel to assess potential additive effects and synergy. Although one of these agents, calcitriol, has repeatedly demonstrated antiproliferative properties against cancer of the prostate, breast, colon, and lung, the antineoplastic activity of calcitriol requires superphysiologic levels. Unfortunately, chronic exposure to superphysiologic levels of calcitriol causes hypercalcemia and resulting toxicity. Therefore, a host of analogues of calcitriol have been investigated for antineoplastic function, including intermittent dose-intense calcitriol, or DN-101. Because of encouraging results from phase II studies of DN-101 combined with docetaxel, the ASCENT (AIPC Study of Calcitriol Enhancement of Taxotere) phase II trial investigated docetaxel plus DN-101 versus docetaxel plus placebo in 250 men with metastatic AIPC and an abnormal baseline prostate-specific antigen (PSA) level. Although the ASCENT trial did not achieve its primary endpoint for increased PSA response, there was a significant trend in PSA response rate in the DN-101 arm. DN-101 in combination with docetaxel seems to improve overall survival and, interestingly, has a favorable safety profile compared with docetaxel alone. The DN-101/docetaxel combination is currently being studied in a much larger international trial, ASCENT-2.

  1. Commercially available probiotic drinks containing Lactobacillus casei DN-114001 reduce antibiotic-associated diarrhea.

    Science.gov (United States)

    Dietrich, Christoph G; Kottmann, Tanja; Alavi, Manuela

    2014-11-14

    To investigate the effect of Lactobacillus-containing commercially available probiotic formulations in Germany during antibiotic treatment with an analysis of cost-efficiency. In an observational study, we analyzed the frequency of bowel movements from 258 patients with infections in a primary care hospital in western Germany; 107 of the patients were offered a probiotic drink containing at least 10 billion cultures of Lactobacillus casei DN 114001 b.i.d. The economic analysis was based on the costs of patient isolation vs preventive intake of probiotics. In a second pilot study, two commercially available probiotic drinks with different Lactobacillus casei strains were directly compared in 60 patients in a randomized controlled fashion. In the first study, the incidence of antibiotic-associated diarrhea (AAD) was significantly reduced in the intervention group (6.5% vs 28.4%), and the duration of AAD in days was significantly shorter (1.7 ± 1.1 vs 3.1 ± 2.1). Higher age and creatinine and lower albumin were identified as risk factors for AAD. Ampicillin was the antibiotic with the highest rate of AAD (50%) and with the greatest AAD reduction in the probiotic group (4.2%, relative risk reduction 92%). The economic analysis showed a cost advantage of nearly 60000 €/year in a department of this size. The second study confirmed the preventive effect of the drink with Lactobacillus casei DN114001; however, there were no advantages found for the other tested probiotic drink containing Lactobacillus casei Shirota. In contrast to a drink containing Lactobacillus casei Shirota, a commercially available probiotic drink containing Lactobacillus casei DN 114001 cost-efficiently reduces the prevalence of AAD during antibiotic treatment.

  2. The mouse model of Down syndrome Ts65Dn presents visual deficits as assessed by pattern visual evoked potentials.

    Science.gov (United States)

    Scott-McKean, Jonah Jacob; Chang, Bo; Hurd, Ronald E; Nusinowitz, Steven; Schmidt, Cecilia; Davisson, Muriel T; Costa, Alberto C S

    2010-06-01

    The Ts65Dn mouse is the most complete widely available animal model of Down syndrome (DS). Quantitative information was generated about visual function in the Ts65Dn mouse by investigating their visual capabilities by means of electroretinography (ERG) and patterned visual evoked potentials (pVEPs). pVEPs were recorded directly from specific regions of the binocular visual cortex of anesthetized mice in response to horizontal sinusoidal gratings of different spatial frequency, contrast, and luminance generated by a specialized video card and presented on a 21-in. computer display suitably linearized by gamma correction. ERG assessments indicated no significant deficit in retinal physiology in Ts65Dn mice compared with euploid control mice. The Ts65Dn mice were found to exhibit deficits in luminance threshold, spatial resolution, and contrast threshold, compared with the euploid control mice. The behavioral counterparts of these parameters are luminance sensitivity, visual acuity, and the inverse of contrast sensitivity, respectively. DS includes various phenotypes associated with the visual system, including deficits in visual acuity, accommodation, and contrast sensitivity. The present study provides electrophysiological evidence of visual deficits in Ts65Dn mice that are similar to those reported in persons with DS. These findings strengthen the role of the Ts65Dn mouse as a model for DS. Also, given the historical assumption of integrity of the visual system in most behavioral assessments of Ts65Dn mice, such as the hidden-platform component of the Morris water maze, the visual deficits described herein may represent a significant confounding factor in the interpretation of results from such experiments.

  3. Correcting incompatible DN values and geometric errors in nighttime lights time series images

    Energy Technology Data Exchange (ETDEWEB)

    Zhao, Naizhuo [Texas Tech Univ., Lubbock, TX (United States); Zhou, Yuyu [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Samson, Eric L. [Mayan Esteem Project, Farmington, CT (United States)

    2014-09-19

    The Defense Meteorological Satellite Program’s Operational Linescan System (DMSP-OLS) nighttime lights imagery has proven to be a powerful remote sensing tool to monitor urbanization and assess socioeconomic activities at large scales. However, the existence of incompatible digital number (DN) values and geometric errors severely limit application of nighttime light image data on multi-year quantitative research. In this study we extend and improve previous studies on inter-calibrating nighttime lights image data to obtain more compatible and reliable nighttime lights time series (NLT) image data for China and the United States (US) through four steps: inter-calibration, geometric correction, steady increase adjustment, and population data correction. We then use gross domestic product (GDP) data to test the processed NLT image data indirectly and find that sum light (summed DN value of pixels in a nighttime light image) maintains apparent increase trends with relatively large GDP growth rates but does not increase or decrease with relatively small GDP growth rates. As nighttime light is a sensitive indicator for economic activity, the temporally consistent trends between sum light and GDP growth rate imply that brightness of nighttime lights on the ground is correctly represented by the processed NLT image data. Finally, through analyzing the corrected NLT image data from 1992 to 2008, we find that China experienced apparent nighttime lights development in 1992-1997 and 2001-2008 respectively and the US suffered from nighttime lights decay in large areas after 2001.

  4. Magnetospheric accretion on the fully-convective classical T Tauri star DN Tau

    CERN Document Server

    Donati, JF; Alencar, SHP; Hussain, G; Bouvier, J; Jardine, MM; Menard, F; Dougados, C; Romanova, MM

    2013-01-01

    We report here results of spectropolarimetric observations of the classical T Tauri star DN Tau carried out (at 2 epochs) with ESPaDOnS at the Canada-France-Hawaii Telescope within the `Magnetic Protostars and Planets' programme. We infer that DN Tau, with a photospheric temperature of 3,950+-50 K, a luminosity of 0.8+-0.2 Lsun and a rotation period of 6.32 d, is a ~2Myr-old fully-convective 0.65+-0.05 Msun star with a radius of 1.9+-0.2 Dsun, viewed at an inclination of 35+-10degr. Clear circularly-polarized Zeeman signatures are detected in both photospheric and accretion-powered emission lines, probing longitudinal fields of up to 1.8 kG (in the He1 D3 accretion proxy). Rotational modulation of Zeeman signatures, detected both in photospheric and accretion lines, is different between our 2 runs, providing further evidence that fields of cTTSs are generated by non-stationary dynamos. Using tomographic imaging, we reconstruct maps of the large-scale field, of the photospheric brightness and of the accretion-...

  5. Rational quantum integrable systems of DN type with polarized spin reversal operators

    Directory of Open Access Journals (Sweden)

    B. Basu-Mallick

    2015-09-01

    Full Text Available We study the spin Calogero model of DN type with polarized spin reversal operators, as well as its associated spin chain of Haldane–Shastry type, both in the antiferromagnetic and ferromagnetic cases. We compute the spectrum and the partition function of the former model in closed form, from which we derive an exact formula for the chain's partition function in terms of products of partition functions of Polychronakos–Frahm spin chains of type A. Using a recursion relation for the latter partition functions that we derive in the paper, we are able to numerically evaluate the partition function, and thus the spectrum, of the DN-type spin chain for relatively high values of the number of spins N. We analyze several global properties of the chain's spectrum, such as the asymptotic level density, the distribution of consecutive spacings of the unfolded spectrum, and the average degeneracy. In particular, our results suggest that this chain is invariant under a suitable Yangian group, and that its spectrum coincides with that of a Yangian-invariant vertex model with linear energy function and dispersion relation.

  6. DnA's SONGZHUANG ARTIST RESIDENCE%宋庄艺术公社

    Institute of Scientific and Technical Information of China (English)

    卞皎皎

    2010-01-01

    @@ 2007年8月,由徐甜甜主持的北京DnA建筑事务所开始筹备他们在宋庄的第三个项目:宋庄艺术公社.DnA事务所与宋庄有着不解之缘,它2004年成立之初接手的第一个项目便是宋庄美术馆,此项目获得了2006年WA中国建筑奖.宋庄艺术公社位于宋庄小堡的万盛园艺术区内,这里原来是宋庄美术馆的施工单位堆积建材的场地.艺术公社面朝着一个人工池塘,是一座可以容纳20位艺术家居住和工作的集合型住宅,占地面积1400平米,建筑面积5300平米.2008年3月开始动工,从设计到完工共历时两年.

  7. Precision Measurement of the Neutron Twist-3 Matrix Element dn2: Probing Color Forces

    Energy Technology Data Exchange (ETDEWEB)

    Posik, Matthew; Flay, David; Parno, Diana; Allada, Kalyan; Armstrong, Whitney; Averett, Todd; Benmokhtar, Fatiha; Bertozzi, William; Camsonne, Alexandre; Canan, Mustafa; Cates, Gordon; Chen, Chunhua; Chen, Jian-Ping; Choi, Seonho; Chudakov, Eugene; Cusanno, Francesco; Dalton, Mark; Deconinck, Wouter; De Jager, Cornelis; Deng, Xiaoyan; Deur, Alexandre; Dutta, Chiranjib; El Fassi, Lamiaa; Franklin, Gregg; Friend, Megan; Gao, Haiyan; Garibaldi, Franco; Gilad, Shalev; Gilman, Ronald; Glamazdin, Oleksandr; Golge, Serkan; Gomez, Javier; Guo, Lei; Hansen, Jens-Ole; Higinbotham, Douglas; Holmstrom, Timothy; Huang, J; Hyde, Charles; Ibrahim Abdalla, Hassan; Jiang, Xiaodong; Jin, Ge; Katich, Joseph; Kelleher, Aidan; Kolarkar, Ameya; Korsch, Wolfgang; Kumbartzki, Gerfried; LeRose, John; Lindgren, Richard; Liyanage, Nilanga; Long, Elena; Lukhanin, Oleksandr; Mamyan, Vahe; McNulty, Dustin; Meziani, Zein-Eddine; Michaels, Robert; Mihovilovic, Miha; Moffit, Bryan; Muangma, Navaphon; Nanda, Sirish; Narayan, Amrendra; Nelyubin, Vladimir; Norum, Blaine; Nuruzzaman, nfn; Oh, Yongseok; Peng, Jen-chieh; Qian, Xin; Qiang, Yi; Rakhman, Abdurahim; Riordan, Seamus; Saha, Arunava; Sawatzky, Bradley; Hashemi Shabestari, Mitra; Shahinyan, Albert; Sirca, Simon; Solvignon-Slifer, Patricia; Subedi, Ramesh; Sulkosky, Vincent; Tobias, William; Troth, Wolfgang; Wang, Diancheng; Wang, Y; Wojtsekhowski, Bogdan; Yan, X; Yao, Huan; Ye, Yunxiu; Ye, Zhihong; Yuan, Lulin; Zhan, X; Zhang, Y; Zhang, Y -W; Zhao, Bo; Zheng, Xiaochao

    2014-07-01

    Double-spin asymmetries and absolute cross sections were measured at large Bjorken x (0.25 lte x lte 0.90), in both the deep-inelastic and resonance regions, by scattering longitudinally polarized electrons at beam energies of 4.7 and 5.9 GeV from a transversely and longitudinally polarized 3He target. In this dedicated experiment, the spin structure function g2 on 3He was determined with precision at large x, and the neutron twist-three matrix element dn2 was measured at ?Q2? of 3.21 and 4.32 GeV2/c2, with an absolute precision of about 10?5. Our results are found to be in agreement with lattice QCD calculations and resolve the disagreement found with previous data at ?Q2?= 5 GeV2/c2. Combining dn2 and a newly extracted twist-four matrix element, fn2, the average neutron color electric and magnetic forces were extracted and found to be of opposite sign and about 60 MeV/fm in magnitude.

  8. Changes in gut microbiota in children with atopic dermatitis administered the bacteria Lactobacillus casei DN--114001.

    Science.gov (United States)

    Klewicka, Elzbieta; Cukrowska, Bozena; Libudzisz, Zdzisława; Slizewska, Katarzyna; Motyl, Ilona

    2011-01-01

    Gut microbiota was analyzed in children, aged 6-18 months and suffering from atopic dermatitis before and after 3 month supplementation of their diet with Lactobacillus casei DN--114001 in a dose of 109 cells daily. On completion of this period the total number of fecal Lactobacillus sp. cells decreased from 7.86 Log10 CFU/g to 6.40 Log10 CFU/g. After the next 5 months (without dietary supplementation with the probiotic bacteria) the level of Lactobacillus sp. cells was maintained at the latter value. During the dietary supplementation with the probiotic strain, the level of Bifidobacterium cells was maintained at 6.15-6.89 Log10 CFU/g while after 5 months it decreased to 5.57 Log10 CFU/g. The population of Clostridium sp. was reduced after 3 months of dietary supplementation from 6.49 to 5.83 Log10 CFU/g and was maintained at the latter level during the next 5 months. The dietary supplementation had no effect on populations of Bacteroides sp., Enterococcus sp. and Enterobacteriaceae. Supplementation of children who developed atopic dermatitis with the preparation of Lactobacillus casei DN - 114001 positively affected their gut microbiota in terms of bifidobacteria and clostridia populations.

  9. Quantitative PET imaging of Met-expressing human cancer xenografts with {sup 89}Zr-labelled monoclonal antibody DN30

    Energy Technology Data Exchange (ETDEWEB)

    Perk, Lars R.; Stigter-van Walsum, Marijke; Vosjan, Maria J.W.D.; Leemans, C.R. [VU University Medical Centre, Department of Otolaryngology-Head and Neck Surgery, De Boelelaan 1117, P.O. Box 7057, Amsterdam (Netherlands); Visser, Gerard W.M.; Kloet, Reina W. [VU University Medical Centre, Nuclear Medicine and PET Research, Amsterdam (Netherlands); Giaccone, Giuseppe [National Cancer Institute, Medical Oncology Branch CCR, Bethesda, MD (United States); Albano, Raffaella; Comoglio, Paolo M. [University of Turin Medical School, Division of Molecular Oncology, Institute for Cancer Research and Treatment (IRCC), Turin (Italy); Dongen, Guus A.M.S. van [VU University Medical Centre, Department of Otolaryngology-Head and Neck Surgery, De Boelelaan 1117, P.O. Box 7057, Amsterdam (Netherlands); VU University Medical Centre, Nuclear Medicine and PET Research, Amsterdam (Netherlands)

    2008-10-15

    Targeting the c-Met receptor with monoclonal antibodies (MAbs) is an appealing approach for cancer diagnosis and treatment because this receptor plays a prominent role in tumour invasion and metastasis. Positron emission tomography (PET) might be a powerful tool for guidance of therapy with anti-Met MAbs like the recently described MAb DN30 because it allows accurate quantitative imaging of tumour targeting (immuno-PET). We considered the potential of PET with either {sup 89}Zr-labelled (residualising radionuclide) or {sup 124}I-labelled (non-residualising radionuclide) DN30 for imaging of Met-expressing tumours. The biodistribution of co-injected {sup 89}Zr-DN30 and iodine-labelled DN30 was compared in nude mice bearing either the human gastric cancer line GLT-16 (high Met expression) or the head-and-neck cancer line FaDu (low Met expression). PET images were acquired in both xenograft models up to 4 days post-injection (p.i.) and used for quantification of tumour uptake. Biodistribution studies in GTL-16-tumour-bearing mice revealed that {sup 89}Zr-DN30 achieved much higher tumour uptake levels than iodine-labelled DN30 (e.g. 19.6%ID/g vs 5.3%ID/g, 5 days p.i.), while blood levels were similar, indicating internalisation of DN30. Therefore, {sup 89}Zr-DN30 was selected for PET imaging of GLT-16-bearing mice. Tumours as small as 11 mg were readily visualised with immuno-PET. A distinctive lower {sup 89}Zr uptake was observed in FaDu compared to GTL-16 xenografts (e.g. 7.8%ID/g vs 18.1%ID/g, 3 days p.i.). Nevertheless, FaDu xenografts were also clearly visualised with {sup 89}Zr-DN30 immuno-PET. An excellent correlation was found between PET-image-derived {sup 89}Zr tumour uptake and ex-vivo-assessed {sup 89}Zr tumour uptake (R {sup 2} = 0.98). The long-lived positron emitter {sup 89}Zr seems attractive for PET-guided development of therapeutic anti-c-Met MAbs. (orig.)

  10. Early-onset diabetic E1-DN mice develop albuminuria and glomerular injury typical of diabetic nephropathy.

    Science.gov (United States)

    Hyvönen, Mervi E; Dumont, Vincent; Tienari, Jukka; Lehtonen, Eero; Ustinov, Jarkko; Havana, Marika; Jalanko, Hannu; Otonkoski, Timo; Miettinen, Päivi J; Lehtonen, Sanna

    2015-01-01

    The transgenic E1-DN mice express a kinase-negative epidermal growth factor receptor in their pancreatic islets and are diabetic from two weeks of age due to impaired postnatal growth of β-cell mass. Here, we characterize the development of hyperglycaemia-induced renal injury in the E1-DN mice. Homozygous mice showed increased albumin excretion rate (AER) at the age of 10 weeks; the albuminuria increased over time and correlated with blood glucose. Morphometric analysis of PAS-stained histological sections and electron microscopy images revealed mesangial expansion in homozygous E1-DN mice, and glomerular sclerosis was observed in the most hyperglycaemic mice. The albuminuric homozygous mice developed also other structural changes in the glomeruli, including thickening of the glomerular basement membrane and widening of podocyte foot processes that are typical for diabetic nephropathy. Increased apoptosis of podocytes was identified as one mechanism contributing to glomerular injury. In addition, nephrin expression was reduced in the podocytes of albuminuric homozygous E1-DN mice. Tubular changes included altered epithelial cell morphology and increased proliferation. In conclusion, hyperglycaemic E1-DN mice develop albuminuria and glomerular and tubular injury typical of human diabetic nephropathy and can serve as a new model to study the mechanisms leading to the development of diabetic nephropathy.

  11. Early-Onset Diabetic E1-DN Mice Develop Albuminuria and Glomerular Injury Typical of Diabetic Nephropathy

    Directory of Open Access Journals (Sweden)

    Mervi E. Hyvönen

    2015-01-01

    Full Text Available The transgenic E1-DN mice express a kinase-negative epidermal growth factor receptor in their pancreatic islets and are diabetic from two weeks of age due to impaired postnatal growth of β-cell mass. Here, we characterize the development of hyperglycaemia-induced renal injury in the E1-DN mice. Homozygous mice showed increased albumin excretion rate (AER at the age of 10 weeks; the albuminuria increased over time and correlated with blood glucose. Morphometric analysis of PAS-stained histological sections and electron microscopy images revealed mesangial expansion in homozygous E1-DN mice, and glomerular sclerosis was observed in the most hyperglycaemic mice. The albuminuric homozygous mice developed also other structural changes in the glomeruli, including thickening of the glomerular basement membrane and widening of podocyte foot processes that are typical for diabetic nephropathy. Increased apoptosis of podocytes was identified as one mechanism contributing to glomerular injury. In addition, nephrin expression was reduced in the podocytes of albuminuric homozygous E1-DN mice. Tubular changes included altered epithelial cell morphology and increased proliferation. In conclusion, hyperglycaemic E1-DN mice develop albuminuria and glomerular and tubular injury typical of human diabetic nephropathy and can serve as a new model to study the mechanisms leading to the development of diabetic nephropathy.

  12. Autosomal dominant cerebellar ataxia, deafness, and narcolepsy (ADCA-DN) associated with progressive cognitive and behavioral deterioration.

    Science.gov (United States)

    Walker, Lisa A S; Bourque, Pierre; Smith, Andra M; Warman Chardon, Jodi

    2017-03-01

    Autosomal dominant cerebellar ataxia with deafness and narcolepsy (ADCA-DN) is an emerging syndrome caused by mutations in the C-terminus end of the TS domain of the DNMT1 gene. ADCA-DN is also associated with sensorimotor polyneuropathy, extrapyramidal, and dysautonomic signs, as well as dementia. Little has been reported about the progressive cognitive impairment associated with ADCA-DN. Our objective is to provide a detailed characterization of the cognitive profile of ADCA-DN. Three members of a kindred with ADCA-DN underwent comprehensive neuropsychological testing and neuroimaging. At baseline, 2 individuals demonstrated cognitive profiles with executive difficulties in some areas consistent with frontal-system dysfunction behaviorally and on standardized testing. The third individual was further in the disease course and exhibited more globally impaired cognition consistent with a diagnosis of dementia. This family demonstrated progressive neurodegeneration beginning with isolated areas of executive dysfunction and leading to globally impaired cognition and dementia. Cognitive decline occurred in parallel with neurological deterioration. The cognitive profile is similar to case reports of other individuals with an allelic neurological phenotype, Hereditary Sensory Autonomic Neuropathy 1E, also caused by DNMT1 mutations. (PsycINFO Database Record (c) 2017 APA, all rights reserved).

  13. Significance of M2 and E3 transitions for $4p^54d^{N+1}$ and $4p^64d^{N-1}4f$ configuration metastable level lifetimes

    CERN Document Server

    Karpuškienė, R; Kisielius, R

    2015-01-01

    Magnetic quadrupole and electric octupole transitions from the configurations $4p^54d^{N+1}$ and $4p^64d^{N-1}4f$ were calculated along with magnetic dipole, electric dipole and electric quadrupole radiative transitions in quasirelativistic Hartree-Fock approximation. Their significance in determining the metastable level radiative lifetimes was investigated along several isoelectronic sequences for the ions from $Z=50$ to $Z=92$. Strontium-like ions, zirconium-like ions, molybdenum-like ions and rhodium-like ions were studied comprehensively. Remaining isoelectronic sequences with the ground configuration $4d^{N}$ ($N=1,3,5,7,8,10$) were also reviewed albeit in less detail. A systematic trends of determined total radiative lifetimes were studied. The importance of magnetic quadrupole and electric octupole transitions from metastable levels of ions from these isoelectronic sequences was investigated and discussed. Inclusion of such transitions of higher multipole order can change theoretical radiative lifetim...

  14. Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN).

    Science.gov (United States)

    Yuan, Yang; Sun, Hong; Sun, Zilin

    2017-06-28

    Advanced glycation end products (AGEs) are pathogenic factors of diabetic nephropathy (DN), causing renal damage in various ways. The aim of this study is to investigate the ectopic lipid accumulation caused by AGEs in human renal tubular epithelial cell line (HK-2) cells and the kidney of type 2 diabetic rats. In vivo study, diabetes was induced in male Sprague-Dawley rats through intraperitoneal injection of high-fat/high-sucrose diet and low-dose streptozocin (STZ). Two weeks after STZ injection, the diabetic rats were randomly divided into two groups, namely, untreated diabetic and Aminoguanidine Hydrochloride (AG, an AGEs formation inhibitor)-treated (100 mg/Kg/day, i.g., for 8 weeks) group. In vitro study, according to the different treatments, HK-2 were divided into 6 groups. Intracellular cholesterol content was assessed by Oil Red O staining and cholesterol enzymatic assay. Expression of mRNA and protein of molecules controlling cholesterol homeostasis in the treated cells was examined by real-time quantitative PCR and western blotting, respectively. SREBP cleavage-activating protein (SCAP) translocation was detected by confocal microscopy. Here we found Nε-(carboxymethyl) lysine (CML, a member of the AGEs family) increased Oil Red O staining and intracellular cholesterol ester (CE) in HK-2 cells; Anti-RAGE (AGEs receptor) reduced lipid droplets and the CE level. A strong staining of Oil Red O was also found in the renal tubules of the diabetic rats, which could be alleviated by AG. CML upregulated both mRNA and protein expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoAR), LDL receptor (LDLr), sterol regulatory element binding protein-2 (SREBP-2) and SCAP, which were inhibited by anti-RAGE. The upregulation of these molecules in the kidney of the diabetic rats was also ameliorated by AG. Furthermore, AG reduced serum and renal CML deposition, and improved urine protein and u-NGAL in type 2 diabetic rats. Overall, these results

  15. DN1(p) circadian neurons coordinate acute light and PDF inputs to produce robust daily behavior in Drosophila.

    Science.gov (United States)

    Zhang, Luoying; Chung, Brian Y; Lear, Bridget C; Kilman, Valerie L; Liu, Yixiao; Mahesh, Guruswamy; Meissner, Rose-Anne; Hardin, Paul E; Allada, Ravi

    2010-04-13

    Daily behaviors in animals are determined by the interplay between internal timing signals from circadian clocks and environmental stimuli such as light. How these signals are integrated to produce timely and adaptive behavior is unclear. The fruit fly Drosophila exhibits clock-driven activity increases that anticipate dawn and dusk and free-running rhythms under constant conditions. Flies also respond to the onset of light and dark with acute increases in activity. Mutants of a novel ion channel, narrow abdomen (na), lack a robust increase in activity in response to light and show reduced anticipatory behavior and free-running rhythms, providing a genetic link between photic responses and circadian clock function. We used tissue-specific rescue of na to demonstrate a role for approximately 16-20 circadian pacemaker neurons, a subset of the posterior dorsal neurons 1 (DN1(p)s), in mediating the acute response to the onset of light as well as morning anticipatory behavior. Circadian pacemaker neurons expressing the neuropeptide PIGMENT-DISPERSING FACTOR (PDF) are especially important for morning anticipation and free-running rhythms and send projections to the DN1(p)s. We also demonstrate that DN1(p)Pdfr expression is sufficient to rescue, at least partially, Pdfr morning anticipation defects as well as defects in free-running rhythms, including those in DN1 molecular clocks. Additionally, these DN1 clocks in wild-type flies are more strongly reset to timing changes in PDF clocks than other pacemaker neurons, suggesting that they are direct targets. Taking these results together, we demonstrate that the DN1(p)s lie at the nexus of PDF and photic signaling to produce appropriate daily behavior.

  16. Protein dynamics associated with failed and rescued learning in the Ts65Dn mouse model of Down syndrome.

    Directory of Open Access Journals (Sweden)

    Md Mahiuddin Ahmed

    Full Text Available Down syndrome (DS is caused by an extra copy of human chromosome 21 (Hsa21. Although it is the most common genetic cause of intellectual disability (ID, there are, as yet, no effective pharmacotherapies. The Ts65Dn mouse model of DS is trisomic for orthologs of ∼55% of Hsa21 classical protein coding genes. These mice display many features relevant to those seen in DS, including deficits in learning and memory (L/M tasks requiring a functional hippocampus. Recently, the N-methyl-D-aspartate (NMDA receptor antagonist, memantine, was shown to rescue performance of the Ts65Dn in several L/M tasks. These studies, however, have not been accompanied by molecular analyses. In previous work, we described changes in protein expression induced in hippocampus and cortex in control mice after exposure to context fear conditioning (CFC, with and without memantine treatment. Here, we extend this analysis to Ts65Dn mice, measuring levels of 85 proteins/protein modifications, including components of MAP kinase and MTOR pathways, and subunits of NMDA receptors, in cortex and hippocampus of Ts65Dn mice after failed learning in CFC and after learning was rescued by memantine. We show that, compared with wild type littermate controls, (i of the dynamic responses seen in control mice in normal learning, >40% also occur in Ts65Dn in failed learning or are compensated by baseline abnormalities, and thus are considered necessary but not sufficient for successful learning, and (ii treatment with memantine does not in general normalize the initial protein levels but instead induces direct and indirect responses in approximately half the proteins measured and results in normalization of the endpoint protein levels. Together, these datasets provide a first view of the complexities associated with pharmacological rescue of learning in the Ts65Dn. Extending such studies to additional drugs and mouse models of DS will aid in identifying pharmacotherapies for effective

  17. Long-term running alleviates some behavioral and molecular abnormalities in Down syndrome mouse model Ts65Dn.

    Science.gov (United States)

    Kida, Elizabeth; Rabe, Ausma; Walus, Marius; Albertini, Giorgio; Golabek, Adam A

    2013-02-01

    Running may affect the mood, behavior and neurochemistry of running animals. In the present study, we investigated whether voluntary daily running, sustained over several months, might improve cognition and motor function and modify the brain levels of selected proteins (SOD1, DYRK1A, MAP2, APP and synaptophysin) in Ts65Dn mice, a mouse model for Down syndrome (DS). Ts65Dn and age-matched wild-type mice, all females, had free access to a running wheel either from the time of weaning (post-weaning cohort) or from around 7 months of age (adult cohort). Sedentary female mice were housed in similar cages, without running wheels. Behavioral testing and evaluation of motor performance showed that running improved cognitive function and motor skills in Ts65Dn mice. However, while a dramatic improvement in the locomotor functions and learning of motor skills was observed in Ts65Dn mice from both post-weaning and adult cohorts, improved object memory was seen only in Ts65Dn mice that had free access to the wheel from weaning. The total levels of APP and MAP2ab were reduced and the levels of SOD1 were increased in the runners from the post-weaning cohort, while only the levels of MAP2ab and α-cleaved C-terminal fragments of APP were reduced in the adult group in comparison with sedentary trisomic mice. Hence, our study demonstrates that Ts65Dn females benefit from sustained voluntary physical exercise, more prominently if running starts early in life, providing further support to the idea that a properly designed physical exercise program could be a valuable adjuvant to future pharmacotherapy for DS.

  18. Efecto de la aplicación de vinazas en las propiedades físicas y la actividad de deshidrogenasas en suelos cultivados con maíz dulce (Zea mays L. Effect of vinasse application on physical properties and dehydrogenases activity in soils under sweet corn (Zea mays L. cultivation

    Directory of Open Access Journals (Sweden)

    Magda Narváez Castillo

    2010-04-01

    Full Text Available Se evaluó el efecto de la aplicación de vinazas en algunas propiedades físicas y en la actividad de las deshidrogenasas en un Entic dystropept y en un Fluventic haplustoll del Valle de Cauca, Colombia. Para suplir los requerimientos de K+ del cultivo de maíz dulce (Zea mays L. se utilizaron seis tratamientos (T1 = 100% de K+ como vinaza, T2 = 100% de K+ con KCl; T3 = 50% de K+ como vinaza y KCl, T4 = 75% de K+ con vinaza y 25% de K+ como KCl, T5 = testigo absoluto sin planta, T6 = testigo más planta que fueron dispuestos en un diseño completamente al azar con cinco repeticiones. En el Entic dystropept la densidad aparente disminuyó y en consecuencia la porosidad total aumentó, mientras que en el Fluventic haplustoll sucedió lo contrario. La actividad de las deshidrogenasas fue significativamente mayor (P The effect of vinasse application was assessed on physical properties and dehydrogenases activity in Entic Dystropept and Fluventic Haplustoll of Valle del Cauca, Colombia. To meet the requirements of K+ in sweet corn (Zea mays L. six treatments were used (T1 = 100% of K+ as vinasse; T2 = 100% of K+ with KCl; T3 = 50% of K+ as vinasse and KCl; T4 = 75% of K+ with vinasse and 25% of K+ as KCl; T5 = absolute control without plants; T6 = control plus plants arranged in a completely randomized design with five replications. In Dystropept Entic bulk density decreased and consequently the total porosity increased, while the opposite happened in Haplustoll Fluventic. The dehydrogenase activity was significantly higher (P <0.05 in the Dystropept Entic than in Haplustoll Fluventic presenting low values. The results showed that the changes that are generated as a result of the application of vinasse are different for each type of soil.

  19. Deficiencia de glucosa 6-fostato deshidrogenasa en hombres sanos y en pacientes maláricos; Turbo (Antioquia, Colombia Deficiency of glucose-6-phosphate dehydrogenase in healthy men and malaria patients; Turbo (Antioquia, Colombia

    Directory of Open Access Journals (Sweden)

    Jaime Carmona-Fonseca

    2008-06-01

    Full Text Available INTRODUCCIÓN: En América Latina la deficiencia de glucosa 6-fosfato deshidrogenasa (d-G6PD ha sido poco estudiada y en Colombia solo conocemos tres publicaciones antiguas. Urge conocer más la prevalencia de d-G6PD, sobre todo ahora que el tratamiento de la malaria vivax plantea aumentar la dosis diaria o total de primaquina. OBJETIVO: Medir la prevalencia de d-G6PD en poblaciones masculina sana y de enfermos con malaria por Plasmodium vivax, en Turbo (Urabá, departamento de Antioquia, Colombia. METODOLOGÍA: Encuestas de prevalencia, para evaluar la G6PD en dos poblaciones de Turbo (Antioquia: hombres sanos; hombres y mujeres con malaria vivax. Se trabajó con muestras diseñadas con criterios estadístico-epidemiológicos. La actividad enzimática se midió con el método normalizado de Beutler para valorar la G6PD en hemolizados. RESULTADOS: Entre los hombres sanos (n = 508, el intervalo de confianza 95% para el promedio (IC95% estuvo entre 4,15 y 4,51 UI/g hemoglobina y 14,8% presentaron valores por debajo del "límite normal" de INTRODUCTION: Glucose-6-phosphate dehydrogenase (G6PD deficiency in Latin America has not been fully studied and in Colombia only three outdated publications are known. Recent information on the prevalence of G6PD deficiency is required now, because the recommended treatment of vivax malaria requires higher daily or total doses of primaquine. OBJECTIVE: To measure the prevalence of G6PD in a healthy male population and in a Plasmodium vivax infected population in Turbo (Urabá, Antioquia Department, Colombia. METHOD: Prevalence survey to evaluate G6PD in two populations of Turbo (Antioquia: healthy male; male and female with vivax malaria. The work was carried out on population samples selected using statistical and epidemiological criteria. Enzyme activity was measured using Beutler's normalized method to evaluate G6PD after hemolysis. RESULTS: For the healthy male group (n = 508, and with a 95% confidence

  20. Actividad de la lactato deshidrogenasa en fluído crevicular gingival y saliva en fumadores con periodontitis crónica Lactate dehidrogenase activity in gingival crevicular fluid and saliva of smoker with chronic periodontitis

    Directory of Open Access Journals (Sweden)

    E.R. Leyva Huerta

    2009-04-01

    Full Text Available Existe un alto porcentaje de enfermedad periodontal atribuido al hábito del tabaco. La respuesta del organismo a esta enfermedad incluye la liberación de enzimas intracelulares relacionadas con la muerte y destrucción celular, como la Lactato Deshidrogenasa (LDH. Objetivo: Comparar los valores de LDH en fluido crevicular gingival (FCG y saliva de pacientes fumadores y no fumadores con Periodontitis crónica (PC. Metodología: Participaron 15 pacientes hombres mayores de 30 años; 6 fumadores, 6 no fumadores con PC y 3 sujetos control. Se recolectaron muestras de saliva total y de FCG de bolsas periodontales de = 6 mm. El volumen del FCG se determino pesando las puntas de papel antes y después del muestreo obteniendo los valores en gramos y convertidos a unidades de volumen (ml. La actividad de LDH se determino por medio de espectrofotometría. Los resultados se convirtieron a unidades de actividad enzimática expresados en mM. El volumen de FCG fue de 0,78 µL en sujetos sanos, en no fumadores con PC 1.12 y en fumadores con PC 1,32. Los valores de LDH correspondieron a; saliva de sujetos sanos 0,845, en no fumadores con PC 1,325 y en fumadores con PC 1,7895 mM. En FCG la actividad fue 0,4568 en sujetos sanos, en no fumadores con PC 0,987 y en fumadores con PC 1,2546 mM. Conclusiones: Las técnicas utilizadas permitieron determinar diferencia entre los valores de la actividad de LDH en ambos fluidos en fumadores y no fumadores con PC.The response of the body to periodontal disease includes the production of intra-cellular enzymes like Lactate Dehydrogenase (LDH which is released by damaged periodontal cells. The LDH can be found in the gingival crevicular fluid (GCF and saliva as a result of cellular death and damage. Objective: To compare LDH activity in GCF and saliva of smoker and non-smoker patients with Chronic Periodontitis. Methods: 15 male patients (30 to 50 years of age participated in this study; 6 smokers with Chronic Periodontitis

  1. Millimeter-Wave Spectroscopy and Global Analysis of the Lowest Eight Vibrational States of Deuterated Hydrazoic Acid (DN_3)

    Science.gov (United States)

    Amberger, Brent K.; Woods, R. Claude; Esselman, Brian J.; McMahon, Robert J.

    2015-06-01

    Hydrazoic acid (HN_3) and DN_3 have qualitatively different rotational spectra, owing in large part to a substantial difference in their A rotational constants (345 GHz for DN_3 vs 611 GHz for HN_3). Like HN_3, DN_3 has six fundamental vibrational modes, of which four are visible in our millimeter-wave spectra at room temperature. Between 240 and 450 GHz, many pure rotational transitions for the ground vibrational state, νb{5} (496 cm-1), νb{6} (586 cm-1), νb{4} (955 cm-1), νb{3} (1197 cm-1), the first overtones of νb{5} and νb{6}, and the combination νb{5}+νb{6} have been observed and assigned. Because DN_3 is a light molecule, the rotational energy levels are widely spaced, leading to numerous interactions between rotational states of different vibrational modes. We have drawn on a wealth of previously published ro-vibrational data from high resolution FTIR spectra in our efforts to understand these perturbations. The centrifugal distortion interaction between νb{5} and the ground state of DN_3 is less dramatic than in HN_3 but still significant. DN_3 shows the same set of Coriolis interactions as does HN_3, but again, their magnitude is generally smaller. In DN_3 the νb{4} state is at slightly lower energy than 2νb{5}, instead of being nearly degenerate with νb{5}+νb{6} as is the case for HN_3. Therefore, there are strong local interactions between 2νb{5} and νb{4}, as well as between νb{3} and 2νb{6}. A notable advantage in solving the DN_3 problem compared to HN_3 is the substantial increase in the number and diversity of observable b-type lines in our frequency region. Furthermore, the smaller A value permits higher K states to be observed due to a more gradual decrease in state populations. Ground state observations have been extended through K = 11 and through J = 50. Pickett's SPFIT has been employed to carry out multi-state fits using combined datasets of our millimeter-wave data and the published FTIR data. J. Bendtsen and F. M

  2. Review of the origin of sulphur in DN-1 discharge and its implication for future development, Dauin prospect, central Philippines

    Energy Technology Data Exchange (ETDEWEB)

    Bayrante, L.F.; Hermoso, D.Z.; Candelaria, M.R. [PNOC-Energy Development Corp., Fort Bonifacio Makati City (Philippines)] [and others

    1997-12-31

    Well DN-1, the first exploratory well of the Dauin geothermal prospect discharged in 1983 substantial quantities of sulphur with a near-neutral pH fluid (pH 6.4 to 7.2) containing maximum chloride levels of 3,300 mg/kg, SO{sub 4} of 300 mg/kg; and high CO{sub 2} and H{sub 2}S relative to the production wells in Palinpinon Field to the north. The chemistry of DN-1 discharge-fluid and the origin of sulphur have been the cause of apprehension for any future development due to concerns on the presence of a possible acid resource southeast of Cuernos de Negros. A reinterpretation of the previous and new surface data was undertaken in 1992 and 1996, including the origin of sulphur, to evaluate the potential of Dauin for development. The results indicate that the sulphur in DN-1 is formed from partial oxidation of hydrogen sulphide derived from the neutralised-acid fluids formed by sulphur hydrolysis at shallow levels but distant from DN-1. The study argues for the presence of near neutral exploitable resource in the prospect area.

  3. Monoacylglycerol lipase inhibitor JZL184 improves behavior and neural properties in Ts65Dn mice, a model of down syndrome.

    Directory of Open Access Journals (Sweden)

    Larisa V Lysenko

    Full Text Available Genetic alterations or pharmacological treatments affecting endocannabinoid signaling have profound effects on synaptic and neuronal properties and, under certain conditions, may improve higher brain functions. Down syndrome (DS, a developmental disorder caused by triplication of chromosome 21, is characterized by deficient cognition and inevitable development of the Alzheimer disease (AD type pathology during aging. Here we used JZL184, a selective inhibitor of monoacylglycerol lipase (MAGL, to examine the effects of chronic MAGL inhibition on the behavioral, biochemical, and synaptic properties of aged Ts65Dn mice, a genetic model of DS. In both Ts65Dn mice and their normosomic (2N controls, JZL184-treatment increased brain levels of 2-arachidonoylglycerol (2-AG and decreased levels of its metabolites such as arachidonic acid, prostaglandins PGD2, PGE2, PGFα, and PGJ2. Enhanced spontaneous locomotor activity of Ts65Dn mice was reduced by the JZL184-treatement to the levels observed in 2N animals. Deficient long-term memory was also improved, while short-term and working types of memory were unaffected. Furthermore, reduced hippocampal long-term potentiation (LTP was increased in the JZL184-treated Ts65Dn mice to the levels observed in 2N mice. Interestingly, changes in synaptic plasticity and behavior were not observed in the JZL184-treated 2N mice suggesting that the treatment specifically attenuated the defects in the trisomic animals. The JZL184-treatment also reduced the levels of Aβ40 and Aβ42, but had no effect on the levels of full length APP and BACE1 in both Ts65Dn and 2N mice. These data show that chronic MAGL inhibition improves the behavior and brain functions in a DS model suggesting that pharmacological targeting of MAGL may be considered as a perspective new approach for improving cognition in DS.

  4. German-translated Norfolk quality of life (QOL-DN) identifies the same factors as the English version of the tool and discriminates different levels of neuropathy severity.

    Science.gov (United States)

    Vinik, Etta J; Paulson, James F; Ford-Molvik, Stephanie L; Vinik, Aaron I

    2008-11-01

    The development and validation of Norfolk QOL-DN, a fiber-specific, quality-of-life tool for diabetic neuropathy, was published previously (Part 1). This study (Part 2) defines the psychometric properties of the German-translated Norfolk QOL-DN in a large multicenter (96 sites) population with neuropathy ranging from minimal to severe, comparing them with those in the original English/American version in a 30-center European/North American population with mild neuropathy; determines the power of the German-translated version in a five-staged diabetic peripheral neuropathy (DPN) German population to discriminate different levels of neuropathy severity; and establishes factors having the greatest impact on QOL. One hundred eighty-six German patients were assessed: asymptomatic of DPN (n = 40), symptomatic (n = 46), DN with foot-ulcer history (n = 32), DN with amputations (n = 22), and DN amputation history (n = 46). German-translated Norfolk QOL-DN was administered to 177 patients with staged DN complications. German-translated Norfolk QOL-DN data were compared with QOL-DN data from the European/American study of 379 mild neuropathy patients. Exploratory factor analysis assessed factor structure consistency in the translated instrument. Ordinal regression analysis (polytomous universal model) was used to evaluate the association between factor scores and complication stages. The German translation identified the same five factors in more advanced neuropathy as in the English mild neuropathy population. Total QOL scores differed among each of the five neuropathy severity groups [analysis of variance p DN discriminates levels of neuropathy within and between populations. Physical functioning and ADL are the most important determinants of QOL. Early occurrence of orthostasis suggests a redefinition of autonomic neuropathy to be more symptom inclusive.

  5. RETRACTED: Association between the ACE I/D gene polymorphism and T2DN susceptibility: The risk of T2DM developing into T2DN in the Asian population.

    Science.gov (United States)

    Zhong, Weiqiang; Jiang, Zongpei; Zhou, Tian-Biao

    2015-12-01

    This article has been included in a multiple retraction: Weiqiang Zhong, Zongpei Jiang, and Tian-Biao Zhou Association between the ACE I/D gene polymorphism and T2DN susceptibility: The risk of T2DM developing into T2DN in the Asian population Journal of Renin-Angiotensin-Aldosterone System 1470320314566019, first published on January 26, 2015 doi: 10.1177/1470320314566019 This article has been retracted at the request of the Editors and the Publisher. After conducting a thorough investigation, SAGE found that the submitting authors of a number of papers published in the Journal of the Renin-Angiotensin Aldosterone System ( JRAAS) (listed below) had supplied fabricated contact details for their nominated reviewers. The Editors accepted these papers based on the reports supplied by the individuals using these fake reviewer email accounts. After concluding that the peer review process was therefore seriously compromised, SAGE and the journal Editors have decided to retract all affected articles. Online First articles (these articles will not be published in an issue) Wenzhuang Tang, Tian-Biao Zhou, and Zongpei Jiang Association of the angiotensinogen M235T gene polymorphism with risk of diabetes mellitus developing into diabetic nephropathy Journal of Renin-Angiotensin-Aldosterone System 1470320314563426, first published on December 18, 2014 doi: 10.1177/1470320314563426 Tian-Biao Zhou, Hong-Yan Li, Zong-Pei Jiang, Jia-Fan Zhou, Miao-Fang Huang, and Zhi-Yang Zhou Role of renin-angiotensin-aldosterone system inhibitors in radiation nephropathy Journal of Renin-Angiotensin-Aldosterone System 1470320314563424, first published on December 18, 2014 doi: 10.1177/1470320314563424 Weiqiang Zhong, Zongpei Jiang, and Tian-Biao Zhou Association between the ACE I/D gene polymorphism and T2DN susceptibility: The risk of T2DM developing into T2DN in the Asian population Journal of Renin-Angiotensin-Aldosterone System 1470320314566019, first published on January 26, 2015 doi: 10

  6. RETRACTED: Relationship between the ACE I/D gene polymorphism and T1DN susceptibility/risk of T1DM developing into T1DN in the Caucasian population.

    Science.gov (United States)

    Zhou, Tian-Biao; Guo, Xue-Feng; Jiang, Zongpei; Li, Hong-Yan

    2015-12-01

    The following article has been included in a multiple retraction: Tian-Biao Zhou, Xue-Feng Guo, Zongpei Jiang, and Hong-Yan Li Relationship between the ACE I/D gene polymorphism and T1DN susceptibility/risk of T1DM developing into T1DN in the Caucasian population Journal of Renin-Angiotensin-Aldosterone System 1470320314563425, first published on February 1, 2015 doi: 10.1177/1470320314563425 This article has been retracted at the request of the Editors and the Publisher. After conducting a thorough investigation, SAGE found that the submitting authors of a number of papers published in the Journal of the Renin-Angiotensin Aldosterone System ( JRAAS) (listed below) had supplied fabricated contact details for their nominated reviewers. The Editors accepted these papers based on the reports supplied by the individuals using these fake reviewer email accounts. After concluding that the peer review process was therefore seriously compromised, SAGE and the journal Editors have decided to retract all affected articles. Online First articles (these articles will not be published in an issue) Wenzhuang Tang, Tian-Biao Zhou, and Zongpei Jiang Association of the angiotensinogen M235T gene polymorphism with risk of diabetes mellitus developing into diabetic nephropathy Journal of Renin-Angiotensin-Aldosterone System 1470320314563426, first published on December 18, 2014 doi: 10.1177/1470320314563426 Tian-Biao Zhou, Hong-Yan Li, Zong-Pei Jiang, Jia-Fan Zhou, Miao-Fang Huang, and Zhi-Yang Zhou Role of renin-angiotensin-aldosterone system inhibitors in radiation nephropathy Journal of Renin-Angiotensin-Aldosterone System 1470320314563424, first published on December 18, 2014 doi: 10.1177/1470320314563424 Weiqiang Zhong, Zongpei Jiang, and Tian-Biao Zhou Association between the ACE I/D gene polymorphism and T2DN susceptibility: The risk of T2DM developing into T2DN in the Asian population Journal of Renin-Angiotensin-Aldosterone System 1470320314566019, first published on January

  7. Mould Design of DN200 Water Curtain Spool and its Investment Casting%DN200水幕阀芯精密铸造模具及工艺设计

    Institute of Scientific and Technical Information of China (English)

    范修谦; 孙伟涛; 周琳

    2015-01-01

    Through analyzing shape ,structure of DN200 water curtain spool and its investment casting process,investment casting process was designed.For mould design,three-pushing mould was design, and precision position was realized by a cone-shape position ring as position joint.During wax pattern making process,according to the injecting volume,through hand-made lots of irregular hard wax cluster to add into cavity of the mould and then injecting,inadequate injecting volume can be overcome.In addi-tion,through adding cold wax strip,the shrinkage deformation is decreased to improve the dimension precision of the castings,so the high quality parts were produced successfully.%通过DN200水幕阀芯的形状、结构、精密铸造工艺分析,合理设计了DN200水幕阀芯蜡模,方案:模具采用三开模,通过一个锥形定位环作为定位连接的纽带精确定位。蜡模制造时,根据射蜡机的最大射蜡量,手工制作相当数量的不规则形状硬蜡团,添加到模具的型腔中再合模射蜡,从而解决了射蜡机射蜡量不足的问题。同时由于添加了冷蜡条,减少了蜡件的收缩变形,从而提高了DN200水幕阀芯蜡件的尺寸精度。

  8. 滨河西路DN2000 mm供水管线抢修方案浅议%Discussion on Rush-repair Scheme for DN 2 000 mm Water Pipeline on Binhe West Road

    Institute of Scientific and Technical Information of China (English)

    李伟

    2010-01-01

    DN2 000mm供水管线是太原市的主输水管线,管径比较大,修理比较困难.介绍了风2 000mm供水管线漏点的确定方法,阐述了抢修方案与抢修流程,为大口径输水管线的维修提供了经验借鉴.

  9. DN2菌降解烟碱的动力学及其应用研究%Study on the Characteristics of Nicotine Degradation by Strain DN2 and its Application

    Institute of Scientific and Technical Information of China (English)

    袁勇军; 陆兆新; 别小妹; 吕凤霞

    2006-01-01

    研究了菌株DN2降解烟碱的特性和对烟草废弃物中烟碱的降解情况.结果表明,该菌降解烟碱的最适条件为接种量5%,温度30℃,初始pH值6.5.在该条件下,对初始烟碱浓度为500mg/L的降解过程进行考察.结果表明,未经烟碱诱导的降解曲线呈倒S曲线,半衰期为17.43h;经烟碱诱导的降解曲线符合Eckenfelder动力学模型,半衰期为4.10h.添加0.1%(质量分数)葡萄糖,可提高菌株DN2的烟碱耐受浓度,达5 000mg/L.菌株DN2能够降解烟草废弃物水提液中的烟碱(烟碱含量约为2 220mg/L),60h时烟碱的降解率为95.22%,表明该菌在治理烟碱污染环境方面具有应用价值.

  10. The production of alpha/beta and gamma/delta double negative (DN) T-cells and their role in the maintenance of pregnancy.

    Science.gov (United States)

    Chapman, John C; Chapman, Fae M; Michael, Sandra D

    2015-07-12

    The ability of the thymus gland to convert bone marrow-derived progenitor cells into single positive (SP) T-cells is well known. In this review we present evidence that the thymus, in addition to producing SP T-cells, also has a pathway for the production of double negative (DN) T-cells. The existence of this pathway was noted during our examination of relevant literature to determine the cause of sex steroid-induced thymocyte loss. In conducting this search our objective was to answer the question of whether thymocyte loss is the end product of a typical interaction between the reproductive and immune systems, or evidence that the two systems are incompatible. We can now report that "thymocyte loss" is a normal process that occurs during the production of DN T-cells. The DN T-cell pathway is unique in that it is mediated by thymic mast cells, and becomes functional following puberty. Sex steroids initiate the development of the pathway by binding to an estrogen receptor alpha located in the outer membrane of the mast cells, causing their activation. This results in their uptake of extracellular calcium, and the production and subsequent release of histamine and serotonin. Lymphatic vessels, located in the subcapsular region of the thymus, respond to the two vasodilators by undergoing a substantial and preferential uptake of gamma/delta and alpha/beta DN T- cells. These T- cells exit the thymus via efferent lymphatic vessels and enter the lymphatic system.The DN pathway is responsible for the production of three subsets of gamma/delta DN T-cells and one subset of alpha/beta DN T-cells. In postpubertal animals approximately 35 % of total thymocytes exit the thymus as DN T-cells, regardless of sex. In pregnant females, their levels undergo a dramatic increase. Gamma/delta DN T-cells produce cytokines that are essential for the maintenance of pregnancy.

  11. The $DN$, $\\pi \\Sigma_c$ interaction in finite volume and the $\\Lambda_c(2595)$ resonance

    CERN Document Server

    Xie, Ju-Jun

    2012-01-01

    In this work the interaction of the coupled channels $DN$ and $\\pi \\Sigma_c$ in an SU(4) extrapolation of the chiral unitary theory, where the $\\Lambda_c(2595)$ resonance appears as dynamically generated from that interaction, is extended to produce results in finite volume. Energy levels in the finite box are evaluated and, assuming that they would correspond to lattice results, the inverse problem of determining the phase shifts in the infinite volume from the lattice results is solved. We observe that it is possible to obtain accurate $\\pi \\Sigma_c$ phase shifts and the position of the $\\Lambda_c(2595)$ resonance, but it requires the explicit consideration of the two coupled channels. We also observe that some of the energy levels in the box are attached to the closed $DN$ channel, such that their use to induce the $\\pi \\Sigma_c$ phase shifts via L\\"uscher's formula leads to incorrect results.

  12. Altered expression of neuropeptides in the primary somatosensory cortex of the Down syndrome model Ts65Dn.

    Science.gov (United States)

    Hernández, Samuel; Gilabert-Juan, Javier; Blasco-Ibáñez, José Miguel; Crespo, Carlos; Nácher, Juan; Varea, Emilio

    2012-02-01

    Down syndrome is the most common genetic disorder associated with mental retardation. Subjects and mice models for Down syndrome (such as Ts65Dn) show defects in the formation of neuronal networks in both the hippocampus and the cerebral cortex. The principal neurons display alterations in the morphology, density and distribution of dendritic spines in the cortex as well as in the hippocampus. Several evidences point to the possibility that the atrophy observed in principal neurons could be mediated by changes in their inhibitory inputs and, in fact, an imbalance between excitation and inhibition has been observed in Ts65Dn mice in these regions, which are crucial for learning and information processing. These animals have an increased density of interneurons in the primary somatosensory cortex, especially of those expressing calretinin and calbindin D-28k. Here, we have analysed the expression and distribution of several neuropeptides in the primary somatosensory cortex of Ts65Dn mice in order to investigate whether these subpopulations of interneurons are affected. We have observed an increase in the total density of somatostatin expressing interneurons and of those expressing VIP in layer IV in Ts65Dn mice. The typology of the somatostatin and VIP interneurons was unaltered as attested by the pattern of co-expression with other markers. Somatostatin immunoreactive neurons co-express mainly D-28k calbindin and VIP expressing interneurons maintain its pattern of co-expression with calcium binding proteins. These alterations, in case they were also present in subjects with Down syndrome, could be related to their impairment in cognitive profile and could be involved in the neurological defects observed in this disorder.

  13. Biodegradation of RDX and MNX with Rhodococcus sp. Strain DN22: New Insights into the Degradation Pathway

    Science.gov (United States)

    2010-11-15

    ENVIRONMENTAL SCIENCE & TECHNOLOGY / VOL. 44, NO. 24, 2010 10.1021/es1023724  2010 American Chemical Society Published on Web 11/24/2010 Report...exclusively; how- FIGURE 1. Time course of aerobic biodegradation of RDX with Rhodococcus sp. DN22 VOL. 44, NO. 24, 2010 / ENVIRONMENTAL SCIENCE & TECHNOLOGY 9...water (H216O) (C) or H218O (D). 9332 9 ENVIRONMENTAL SCIENCE & TECHNOLOGY / VOL. 44, NO. 24, 2010 Degradation of MNX with Rhodococcus sp. strain

  14. Assessing Apoptosis Gene Expression Profiling with a PCR Array in the Hippocampus of Ts65Dn Mice

    Directory of Open Access Journals (Sweden)

    Bin Yu

    2015-01-01

    Full Text Available It is well known that Down syndrome (DS is a condition in which extra genetic material causes delays in the way a child develops, both mentally and physically. Intellectual disability is the foremost and most debilitating trait, which caused loss of cognitive abilities and the development of early onset Alzheimer’s disease (AD. Ts65Dn mice were used in this study. We isolated the hippocampus. First, we used transmission scanning electron microscopy to directly observe the hippocampus and confirm if apoptosis had occurred. Second, we customized a PCR array with 53 genes, including several important genes related to cell apoptosis. Gene expression was detected by RT-PCR. There were varying degrees of changes characteristic of apoptosis in the hippocampus of Ts65Dn mice, which mainly included the following: nuclear membrane thinning, unevenly distributed chromosomes, the production of chromatin crescents, and pyknosis of the nuclei with some nuclear fragmentation. Meanwhile, three genes (API5, AIFM1, and NFκB1 showed changes of expression in the hippocampus of Ts65Dn mice compared with normal mice. Only NFκB1 expression was significantly increased, while the expressions of API5 and AIFM1 were notably decreased. The fold changes in the expression of API5, AIFM1, and NFκB1 were 11.55, 5.94, and 3.11, respectively. However, some well-known genes related to cell apoptosis, such as the caspase family, Bcl-2, Bad, Bid, Fas, and TNF, did not show changes in expression levels. The genes we found which were differentially expressed in the hippocampus of Ts65Dn mice may be closely related to cell apoptosis. PCR array technology can assist in the screening and identification of genes involved in apoptosis.

  15. Fermented milk containing Bifidobacterium lactis DN-173 010 in childhood constipation: a randomized, double-blind, controlled trial.

    Science.gov (United States)

    Tabbers, Merit M; Chmielewska, Ania; Roseboom, Maaike G; Crastes, Nolwenn; Perrin, Catherine; Reitsma, Johannes B; Norbruis, Obbe; Szajewska, Hania; Benninga, Marc A

    2011-06-01

    Constipation is a frustrating symptom affecting 3% of children worldwide. A fermented dairy product containing Bifidobacterium lactis strain DN-173 010 was effective in increasing stool frequency in constipated women. Our aim was to assess the effects of this product in constipated children. In this prospective randomized, double-blind, controlled trial, 159 constipated children (defecation frequency DN-173 010 (n = 79) or a control product (n = 80) twice a day for 3 weeks. The primary endpoint was the change in stool frequency from baseline to after 3 weeks of product consumption. Analyses were by intention to treat. Eleven children did not return to any follow-up visit (5 in the probiotic group, 6 in the control group) and were therefore excluded from the final analysis. Thus, 74 children in each group were analyzed. The change in stool frequency from baseline to after 3 weeks of product consumption increased in both groups, but the difference was not statistically significant (2.9 ± 3.2 in probiotic group versus 2.6 ± 2.6 in control group, P = .35). There were no serious adverse events. In constipated children, the fermented dairy product containing B lactis strain DN-173 010 did increase stool frequency, but this increase was comparable in the control group. There is currently not sufficient evidence to recommend fermented dairy products containing B lactis strain DN-173 010 in this category of patients. Future studies should focus on whether a longer period of probiotic products is more effective in children who have a short history of constipation.

  16. 内腐蚀缺陷尺寸对DN150承插管线承压能力的影响%Analysis of the Effects of Corrosion Defect Size on DN150 Socket Pipes Failure Pressure

    Institute of Scientific and Technical Information of China (English)

    李健健; 雍歧卫; 周仁; 林芳清

    2013-01-01

    为了科学地指导DN150承插管线的使用与管理,采用非线性有限元法对带内腐蚀缺陷的DN150承插管线进行了剩余强度的评估。通过分析和计算,研究了腐蚀缺陷宽度、长度和深度对管线极限承载能力的影响。结果表明:腐蚀缺陷宽度对DN150承插管线的承载能力影响很小,可忽略不计;随着腐蚀缺陷长度的增加,管线的承载能力略微减小,当缺陷长度达到150 mm,管道失效压力基本不变;随着腐蚀缺陷深度的增加,管道失效压力呈线性递减,其对管道失效压力的影响程度远远大于腐蚀缺陷长度。%In order to instruct the scientific use and management of DN150 socket pipes,non-linear finite element method is applied to evaluate the residual strength of DN150 socket pipes with internal corrosion defects. The effects of depth,length and width of corrosion defect on the pipe failure pressure is researched through computational analysis. The results show that the effect of corrosion defect width is minor and can be neglected. With the length of corrosion defects increasing,pipes failure pressure is de-creasing fractionally and remains fairly static when the length reaches 150 mm. With the depth of corrosion defects increasing,pipes failure pressure is decreasing linearly. The impact of corrosion defects depth on pipe failure pressure is considerably greater than that of corrosion defect length.

  17. Effect of a fermented milk containing Bifidobacterium lactis DN-173010 on Chinese constipated women

    Institute of Scientific and Technical Information of China (English)

    Yue-Xin Yang; Mei He; Gang Hu; Jie Wei; Philippe Pages; Xian-Hua Yang; Sophie Bourdu-Naturel

    2008-01-01

    AIM: To investigate the effect of a fermented milk containing Bifidobacterium lactis DN- 173010 and yogurt strains (BIO ) on adult women with constipation in Beijing. METHODS: A total of 135 adult females with constipation were randomly allocated to consume for 2 wk either 100 g of the test fermented milk or 100 g of an acidified milk containing non-living bacteria (control). Stool frequency, defecation condition scores, stool consistency and food intake were recorded at baseline and after 1 and 2 wk in an intention-to-treat population of 126 subjects. In parallel, safety evaluation parameters were performed. RESULTS: At baseline, no differences were found between groups. Following consumption of test product, stool frequency was significantly increased after 1 wk (3.5±1.5 vs 2.4±0.6, P < 0.01) and 2 wk (4.1±1.7 vs 2.4±0.6, P < 0.01), vs baseline. Similarly, after 1 and 2 wk, of test product consumption, defecation condition (1.1±0.9 vs 1.9±1.2, P < 0.01 and 0.8±1.0 vs 1.9±1.2, P < 0.01, respectively) and stool consistency (1.0±0.8 vs 1.5±1.1, P < 0.01 and 0.6±0.8 vs 1.5±1.1, P < 0.01, respectively) were significantly improved. Compared with the control group, stool frequency was also significantly increased (3.5±1.5 vs 2.5± 0.9, P <0.01 and 4.1±1.7 vs 2.6±1.0, P <0.01, respectively), and defecation condition (1.1±0.9 vs 1.6±1.1, P < 0.01 and 0.8±1.0 vs 1.6±1.1, P < 0.01, respectively) and stool consistency (1.0±0.8 vs 1.4±1.0, P < 0.05 and 0.6±0.8 vs 1.3±1.0, P < 0.01, respectively) significantly decreased after 1 and 2 wk of product consumption. During the same period, food intake did not change between the two groups, and safety parameters of the subjects were within normal ranges. CONCLUSION: This study suggests a beneficial effect of a fermented milk containing B. lactis DN-173010 on stool frequency, defecation condition and stool consistency in adult women with constipation constipated women after 1 and 2 wk of consumption.

  18. Chronic up-regulation of the SHH pathway normalizes some developmental effects of trisomy in Ts65Dn mice.

    Science.gov (United States)

    Dutka, Tara; Hallberg, Dorothy; Reeves, Roger H

    2015-02-01

    Down Syndrome (DS) is a highly complex developmental genetic disorder caused by trisomy for human chromosome 21 (Hsa21). All individuals with DS exhibit some degree of brain structural changes and cognitive impairment; mouse models such as Ts65Dn have been instrumental in understanding the underlying mechanisms. Several phenotypes of DS might arise from a reduced response of trisomic cells to the Sonic Hedgehog (SHH) growth factor. If all trisomic cells show a similar reduced response to SHH, then up-regulation of the pathway in trisomic cells might ameliorate multiple DS phenotypes. We crossed Ptch1tm1Mps/+ mice, in which the canonical SHH pathway is expected to be up-regulated in every SHH-responsive cell due to the loss of function of one allele of the pathway suppressor, Ptch1, to the Ts65Dn DS model and assessed the progeny for possible rescue of multiple DS-related phenotypes. Down-regulation of Ptch produced several previously unreported effects on development by itself, complicating interpretation of some phenotypes, and a number of structural or behavioral effects of trisomy were not compensated by SHH signaling. However, a deficit in a nest-building task was partially restored in Ts;Ptch+/- mice, as were the structural anomalies of the cerebellum seen in Ts65Dn mice. These results extend the body of evidence indicating that reduced response to SHH in trisomic cells and tissues contributes to various aspects of the trisomic phenotype.

  19. Study of Eclipsing Binary and Multiple Systems in OB Associations IV: Cas OB6 Member DN Cas

    CERN Document Server

    Bakis, V; Bilir, S; Eker, Z

    2016-01-01

    An early-type, massive, short-period ($P_{\\rm orb}=2^{\\rm d}.310951$) eclipsing spectroscopic binary DN Cas has been re-visited with new spectral and photometric data. The masses and radii of the components have been obtained as $M_1=19.04\\pm0.07 M_\\odot$, $M_2=13.73\\pm 0.05M_\\odot$ and $R_1=7.22\\pm0.06 R_\\odot$, $R_2=5.79\\pm0.06R_\\odot$, respectively. Both components present synchronous rotation ($V_{\\rm rot1}=160\\,$km s$^{-1}$, $V_{\\rm rot2}=130\\,$km s$^{-1}$) with their orbit. Orbital period analysis yielded a physically bound additional component in the system with a minimum mass of $M_3=0.88 M_\\odot$ orbiting in an eccentric orbit ($e=0.37\\pm0.2$) with an orbital period of $P_{12}=42\\pm9$ yrs. High precision absolute parameters of the system allowed us to derive a distance to DN Cas as 1.7$\\pm$0.2 kpc which locates the system within the borders of the Cas OB6 association (d=1.8 kpc; Mel'nik & Dambis, 2009). The space velocities and the age of DN Cas are in agreement with those of Cas OB6. The age of ...

  20. In vivo delivery of DN:REST improves transcriptional changes of REST-regulated genes in HD mice.

    Science.gov (United States)

    Conforti, P; Mas Monteys, A; Zuccato, C; Buckley, N J; Davidson, B; Cattaneo, E

    2013-06-01

    Current therapeutic strategies for Huntington's disease (HD) are focused on symptom management of disease progression. Transcriptional dysregulation is one of the major characteristics in HD. REST is a transcriptional repressor that silences gene expression through binding to RE1/NRSE sites found in the regulatory regions of numerous neuronal genes. Dysregulation of REST and its targeted genes has been reported in different cell and mouse HD models, as well as in biopsies from human patients. In this work, we characterized transcriptional dysregulation associated with REST in two different HD mouse models and assessed the therapeutic effect of interfering with REST function by overexpressing a dominant-negative form (DN:REST). We show that delivery of DN:REST in the motor cortex restores brain-derived neurotrophic factor (BDNF) mRNA and protein levels by reducing endogenous REST occupancy at the Bdnf locus. Similarly, expression of other REST-regulated genes such as Synapsin I (Syn1), Proenkephalin (Penk1) and Cholinergic receptor muscarinic 4 (Chrm4) were restored to normal levels while non-REST-regulated genes were unaffected. This is the first study conducted to investigate REST's role in vivo in a neurodegenerative disease. Our data show that DN:REST in motor cortex reversed RESTs repressive effects on target genes. However, the lack of therapeutic effect on motor function suggests that a more widespread rescue of REST-regulated sites in the affected brain regions may be necessary.

  1. Determination of Specific Refractive Index Increment (dn/dc)μ at a Constant Chemical Potential, for Solutions of Polymer in Mixed Solvents by the GPC Method

    Institute of Scientific and Technical Information of China (English)

    WANG Qing-guo; LI Xiao-wen; CHENG Rong-shi

    2007-01-01

    A new chromatographic method is described for the determination of specific refractive index increment(dn/dc)μ at a constant chemical potential, for polymer/mixed solvent systems. In this method the (dn/dc) is obtained by measuring the areas of solvated-polymer peaks when the mixed solvent is used as an eluent. Values of(dn/dc)μ for the poly(dimethylsiloxane) (PDMS) -benzene-methanol system, determined by the proposed method are in good agreement with those determined by the conventional dialysis method. The new approach has the advantages of simplicity, fast speed, and high reproducibility. The experimental results for stearic acid-chloroform-methanol system show that this method can also be applied to nonpolymer/mixed solvent systems for the determination of (dn/dc)μ.

  2. Photoinduced multi-electron transfer in the Dn-A system consisting of multi-phthalocyanines linked to one carbon nanotube.

    Science.gov (United States)

    Zhang, Xian-Fu; Cui, Xiaofeng; Liu, Qiang; Zhang, Fushi

    2009-05-14

    A Dn-A model in which a carbon nanotube (nanoscaled electron acceptor A) is attached with a number of electron donors (Dn), i.e. amino phthalocyanines, was employed to reveal the different nature of photoinduced electron transfer (PeT) in the nanoscaled model from that of a usual one-to-one linked D-A system, which is known to form the single-charge separation state D*+-A*-via well-studied single-electron transfer. For the Dn-A system, however, a one-step multi-electron transfer from multi donors to the same acceptor occurs simultaneously to generate a long-lived multiple-charge separation state Dn*+-A*n- (n>1), which is desirable for efficiently mimicking PSII in photosynthesis.

  3. [Clinical evaluations of neoadjuvant chemotherapy with DN and FP regimens for patients with middle or lower thoracic locally advanced esophageal squamous cell carcinoma].

    Science.gov (United States)

    Yang, Hongxia; Yao, Juan; Wen, Hong; Yu, Lan; Liu, Wu; Liang, Hua; Han, Shuhong

    2015-05-19

    To explore the efficacies and side effects of neoadjuvant chemotherapy with DN (docetaxel plus cisplatin) and FP (nedaplatin plus cisplatin) regimens for patients with upper or middle thoracic locally advanced esophageal squamous cell carcinoma. From January 2008 to January 2012, a total of 124 patients with upper or middle thoracic locally advanced esophageal squamous cell carcinoma were randomized into DN group (n = 64) and FP group (n = 60). Both groups received neoadjuvant chemotherapy. The treatment schedule was recycled every 3 weeks. After 2 cycles, those with potential surgical resection underwent surgery. The 2-year overall, locoregional relapse-free and distant metastasis-free survival rates in DN and FP groups were 71.1% and 66.7%, 65.0% and 63.0%, 78.3% and 74.3% respectively (P > 0.05). The incidence of leucopenia was higher in DN group than that in FP group (P DN group. No perioperative mortality occurred with a low incidence of postoperative complications. The rates of overall response, resection, postoperative complications and pathological complete rates response were similar in two groups. And the rates of downstage and R0 resection were significantly higher in DN group than those in FP group (P < 0.05). For patients with middle or lower thoracic locally advanced esophageal squamous cell carcinoma, neoadjuvant chemotherapies of docetaxel and nedaplatin may achieve excellent outcomes in clinical response and 2-year survival rate. And the side effects are clinically acceptable.

  4. INITIAL IN VIVO EXPERIENCE OF PIG ARTERY PATCH TRANSPLANTATION IN BABOONS USING MUTANT MHC (CIITA-DN) PIGS

    Science.gov (United States)

    Iwase, H; Ekser, B; Satyananda, V; Zhou, H; Hara, H; Bajona, P; Wijkstrom, M; Bhama, JK; Long, C; Veroux, M; Wang, Y; Dai, Y; Phelps, C; Ayares, D; Ezzelarab, MB; Cooper, DKC

    2015-01-01

    Background In the pig-to-nonimmunosuppressed baboon artery patch model, a graft from an α1,3-galactosyltransferase gene-knockout pig transgenic for human CD46 (GTKO/CD46) induces a significant adaptive immune response (elicited anti-pig antibody response, increase in T cell proliferation on MLR, cellular infiltration of the graft), which is effectively prevented by anti-CD154mAb-based therapy. Methods As anti-CD154mAb is currently not clinically applicable, we evaluated whether it could be replaced by CD28/B7 pathway blockade or by blockade of both pathways (using belatacept+anti-CD40mAb [2C10R4]). We further investigated whether a patch from a GTKO/CD46 pig with a mutant human MHC class II transactivator (CIITA-DN) gene would allow reduction in the immunosuppressive therapy administered. Results When grafts from GTKO/CD46 pigs were transplanted with blockade of both pathways, a minimal or insignificant adaptive response was documented. When a GTKO/CD46/CIITA-DN graft was transplanted, but no immunosuppressive therapy was administered, a marked adaptive response was documented. In the presence of CD28/B7 pathway blockade (abatacept or belatacept), there was a weak adaptive response that was diminished when compared with that to a GTKO/CD46 graft. Blockade of both pathways prevented an adaptive response. Conclusion Although expression of the mutant MHC CIITA-DN gene was associated with a reduced adaptive immune response when immunosuppressive therapy was inadequate, when blockade of both the CD40/CD154 and CD28/B7 pathways was present, the response even to a GTKO/CD46 graft was suppressed. This was confirmed after GTKO/CD46 heart transplantation in baboons. PMID:25687023

  5. Initial in vivo experience of pig artery patch transplantation in baboons using mutant MHC (CIITA-DN) pigs.

    Science.gov (United States)

    Iwase, H; Ekser, B; Satyananda, V; Zhou, H; Hara, H; Bajona, P; Wijkstrom, M; Bhama, J K; Long, C; Veroux, M; Wang, Y; Dai, Y; Phelps, C; Ayares, D; Ezzelarab, M B; Cooper, D K C

    2015-03-01

    In the pig-to-nonimmunosuppressed baboon artery patch model, a graft from an α1,3-galactosyltransferase gene-knockout pig transgenic for human CD46 (GTKO/CD46) induces a significant adaptive immune response (elicited anti-pig antibody response, increase in T cell proliferation on MLR, cellular infiltration of the graft), which is effectively prevented by anti-CD154mAb-based therapy. As anti-CD154mAb is currently not clinically applicable, we evaluated whether it could be replaced by CD28/B7 pathway blockade or by blockade of both pathways (using belatacept + anti-CD40mAb [2C10R4]). We further investigated whether a patch from a GTKO/CD46 pig with a mutant human MHC class II transactivator (CIITA-DN) gene would allow reduction in the immunosuppressive therapy administered. When grafts from GTKO/CD46 pigs were transplanted with blockade of both pathways, a minimal or insignificant adaptive response was documented. When a GTKO/CD46/CIITA-DN graft was transplanted, but no immunosuppressive therapy was administered, a marked adaptive response was documented. In the presence of CD28/B7 pathway blockade (abatacept or belatacept), there was a weak adaptive response that was diminished when compared with that to a GTKO/CD46 graft. Blockade of both pathways prevented an adaptive response. Although expression of the mutant MHC CIITA-DN gene was associated with a reduced adaptive immune response when immunosuppressive therapy was inadequate, when blockade of both the CD40/CD154 and CD28/B7 pathways was present, the response even to a GTKO/CD46 graft was suppressed. This was confirmed after GTKO/CD46 heart transplantation in baboons. Copyright © 2015 Elsevier B.V. All rights reserved.

  6. Altered Synaptic Marker Abundance in the Hippocampal Stratum Oriens of Ts65Dn Mice is Associated with Exuberant Expression of Versican

    Directory of Open Access Journals (Sweden)

    Matthew D Howell

    2012-01-01

    Full Text Available DS (Down syndrome, resulting from trisomy of chromosome 21, is the most common cause of genetic mental retardation; however, the molecular mechanisms underlying the cognitive deficits are poorly understood. Growing data indicate that changes in abundance or type of CSPGs (chondroitin sulfate proteoglycans in the ECM (extracellular matrix can influence synaptic structure and plasticity. The purpose of this study was to identify changes in synaptic structure in the hippocampus in a model of DS, the Ts65Dn mouse, and to determine the relationship to proteoglycan abundance and/or cleavage and cognitive disability. We measured synaptic proteins by ELISA and changes in lectican expression and processing in the hippocampus of young and old Ts65Dn mice and LMCs (littermate controls. In young (5 months old Ts65Dn hippocampal extracts, we found a significant increase in the postsynaptic protein PSD-95 (postsynaptic density 95 compared with LMCs. In aged (20 months old Ts65Dn hippocampus, this increase was localized to hippocampal stratum oriens extracts compared with LMCs. Aged Ts65Dn mice exhibited impaired hippocampal-dependent spatial learning and memory in the RAWM (radial-arm water maze and a marked increase in levels of the lectican versican V2 in stratum oriens that correlated with the number of errors made in the final RAWM block. Ts65Dn stratum oriens PNNs (perineuronal nets, an extension of the ECM enveloping mostly inhibitory interneurons, were dispersed over a larger area compared with LMC mice. Taken together, these data suggest a possible association with alterations in the ECM and inhibitory neurotransmission in the Ts65Dn hippocampus which could contribute to cognitive deficits.

  7. Altered synaptic marker abundance in the hippocampal stratum oriens of Ts65Dn mice is associated with exuberant expression of versican

    Directory of Open Access Journals (Sweden)

    Paul E Gottschall

    2012-02-01

    Full Text Available DS (Down syndrome, resulting from trisomy of chromosome 21, is the most common cause of genetic mental retardation; however, the molecular mechanisms underlying the cognitive deficits are poorly understood. Growing data indicate that changes in abundance or type of CSPGs (chondroitin sulfate proteoglycans in the ECM (extracellular matrix can influence synaptic structure and plasticity. The purpose of this study was to identify changes in synaptic structure in the hippocampus in a model of DS, the Ts65Dn mouse, and to determine the relationship to proteoglycan abundance and/or cleavage and cognitive disability. We measured synaptic proteins by ELISA and changes in lectican expression and processing in the hippocampus of young and old Ts65Dn mice and LMCs (littermate controls. In young (5 months old Ts65Dn hippocampal extracts, we found a significant increase in the postsynaptic protein PSD-95 (postsynaptic density 95 compared with LMCs. In aged (20 months old Ts65Dn hippocampus, this increase was localized to hippocampal stratum oriens extracts compared with LMCs. Aged Ts65Dn mice exhibited impaired hippocampal-dependent spatial learning and memory in the RAWM (radial-arm water maze and a marked increase in levels of the lectican versican V2 in stratum oriens that correlated with the number of errors made in the final RAWM block. Ts65Dn stratum oriens PNNs (perineuronal nets, an extension of the ECM enveloping mostly inhibitory interneurons, were dispersed over a larger area compared with LMC mice. Taken together, these data suggest a possible association with alterations in the ECM and inhibitory neurotransmission in the Ts65Dn hippocampus which could contribute to cognitive deficits.

  8. DNM1DN: a new class of paralogous genomic segments (duplicons) with highly conserved copies on chromosomes Y and 15.

    Science.gov (United States)

    Makrinou, E; Fox, M; Wolfe, J; Cameron, J; Taylor, K; Edwards, Y H

    2004-03-01

    Screening a testis cDNA selection library for Y-linked genes yielded 79 cDNAs. Of these, 9 matched the 3' region of the dynamin 1 gene (DNM1) on chromosome 9q34 with >90% identity. Fluoresence in situ hybridisation and PCR amplification were used to localise a large number of DNM1-like sequences to human chromosomes 15 and Y. PCR amplification of overlapping Y-linked YACs allowed a more accurate mapping of the Y-linked DNM1-like cDNAs to a euchromatic locus in close proximity to heterochromatin at Yq11.23. A search of the genome database identified 64 highly homologous copies of the DNM1 fragment. Most of these copies were localised to chromosomes 15 and Y, but others mapped to chromosomes 5, 8, 10, 12, 19 and 22. These sequences exhibit all the major features of a duplicon and have been designated DNM1DN (DNM1 duplicon). Evolutionary studies using fluorescence in situ hybridisation indicate that transposition of the DNM1DN sequence to chromosome 15 took place earlier in primate evolution than the transposition to the Y chromosome. The translocation to the Y took place at a time following the divergence of a common ancestor from gorilla, approximately 4-7 million years ago.

  9. Evaluation of endoscopic submucosal dissection using a new endosurgical knife DN-D2718B: a first clinical feasibility study.

    Science.gov (United States)

    Sakaguchi, Yoshiki; Tsuji, Yosuke; Fujishiro, Mitsuhiro; Kataoka, Yosuke; Saito, Itaru; Shichijo, Satoki; Minatsuki, Chihiro; Asada-Hirayama, Itsuko; Yamaguchi, Daisuke; Niimi, Keiko; Ono, Satoshi; Kodashima, Shinya; Yamamichi, Nobutake; Koike, Kazuhiko

    2017-07-01

    Endosurgical devices with injection function have been reported to decrease endoscopic submucosal dissection (ESD) operation times for experts, but the efficacy of these devices for inexperienced endoscopists is unclear. The aim of this study was to evaluate the feasibility of ESD using a novel ESD knife (DN-D2718B). This is a single-center prospective pilot clinical feasibility study. Patients diagnosed with superficial gastrointestinal neoplasms were enrolled. A pre-specified group of ESD trainees with ESD experience on a porcine gastric model and fewer than 30 cases of ESD in their selected fields performed ESD under expert supervision, using the DN-D2718B. En bloc resection rates, R0 resection rates, procedure times, and incidence of intra-operational/post-operational adverse events were assessed. Between June 2015 and January 2016, 13 esophageal, 27 gastric, and 14 colorectal ESD cases were performed per-protocol with mean resection speeds of 10.2, 12.0, and 15.5 mm (2) /min, respectively. There were no intra-operational complications. ESD with this novel knife is feasible even when performed by non-experts.

  10. Productive HIV-1 infection is enriched in CD4(-)CD8(-) double negative (DN) T cells at pleural sites of dual infection with HIV and Mycobacterium tuberculosis.

    Science.gov (United States)

    Meng, Qinglai; Canaday, David H; McDonald, David J; Mayanja-Kizza, Harriet; Baseke, Joy; Toossi, Zahra

    2016-01-01

    A higher human immunodeficiency virus 1 (HIV-1) viral load at pleural sites infected with Mycobacterium tuberculosis (MTB) than in peripheral blood has been documented. However, the cellular source of productive HIV infection in HIV-1/MTB-coinfected pleural fluid mononuclear cells (PFMCs) remains unclear. In this study, we observed significant quantities of HIV-1 p24(+) lymphocytes in PFMCs, but not in peripheral blood mononuclear cells (PBMCs). HIV-1 p24(+) lymphocytes were mostly enriched in DN T cells. Intracellular CD4 expression was detectable in HIV-1 p24(+) DN T cells. HIV-1 p24(+) DN T cells showed lower surface expression of human leukocyte antigen (HLA)-ABC and tetherin than did HIV-1 p24(+) CD4 T cells. Upon in vitro infection of PFMC CD4 T cells from TB mono-infected subjects, Nef- and/or Vpu-deleted HIV mutants showed lower generation of HIV-1 p24(+) DN T cells than the wild-type virus. These data indicate that productively HIV-1-infected DN T cells, generated through down-modulation of surface CD4, likely by HIV-1 Nef and Vpu, are the predominant source of HIV-1 at pleural sites of HIV/MTB coinfection.

  11. ANALYSIS OF THE CONVERGENCE RELATION BETWEEN D-N ALTERNATING PROCESS AND SCHWARTZ ALTERNATING PROCESS%D-N交替法与Schwartz交替法的收敛关系分析

    Institute of Scientific and Technical Information of China (English)

    孙连友; 洪伟

    2003-01-01

    This paper has demonstrated that the Schwartz alternating process must converge if D-N alternating process has converged. Its technique is that the overlapping regions in Schwartz alternating process are considered as independent domains, then Schwartz alternating process could be transformed into D-N alter-nating process. Finally the convergence estimation of Schwartz alternating process could be obtained. The results show that its convergence rate is same as that of D-N alternating process.

  12. Introduction to Singapore DN2 200 Water Supply Pipe Jacking Project%新加坡DN2 200新生水管道顶管工程介绍

    Institute of Scientific and Technical Information of China (English)

    孟繁强; 陈宝强; 刘龙; 孟宪刚; 张雪丽; 孙涛; 阎强; 魏香顺

    2014-01-01

    介绍了新加坡DN2 200新生水管道顶管工程的土质情况、主要顶管设备和配套设备选型、钢筒混凝土复合管的结构及接口方式、管道内涂层、逆作法钢筋混凝土工作井的施工技术等.该工程成功采用并完善逆作法钢筋混凝土工作井的施工工艺,缩短了工期,节省了资金,取得了较好的经济效益.

  13. Ability of probiotic Lactobacillus casei DN 114001 to bind or/and metabolise heterocyclic aromatic amines in vitro.

    Science.gov (United States)

    Nowak, Adriana; Libudzisz, Zdzislawa

    2009-10-01

    Heterocyclic aromatic amines (HCA) are compounds with high mutagenic potential, formed when meat is cooked at high temperatures of 150-300 degrees C. These compounds contribute to development of colon and gastric cancer. Western diet provides a lot of HCA and influences the available substrates for the intestinal microbiota which can activate HCA to direct acting mutagens. On the other hand, lactic acid bacteria existing in the colon and ingested with food including probiotics, may exert an anti-carcinogenic action, but the mechanism is still poorly understood. In the present study we determined the ability of probiotic Lactobacillus casei DN 114001 (Actimel strain) to metabolise or adsorb three HCA: IQ, MelQx and PhIP in vitro. Lactobacilli were cultivated in MRS and in a modified MRS broth with reduced concentrations of nitrogen and carbon (MRS NC), with addition of 25 mug/ml of IQ, MelQx or PhIP. Their concentration after cultivation with L. casei DN 114001 was measured with high-performance liquid chromatography and the genotoxicity was evaluated by the alkaline comet assay. It was measured, that after 24 h cultivation in MRS (cell density was 10(9) cfu/ml), rapid decrease of IQ and PhIP (98-99%) was observed, and the peaks on chromatograms were almost completely reduced. In case of MeIQx the decrease was about 27%. In a modified MRS broth (cell density was 10(8) cfu/ml), the ability to decrease HCA concentration during prolonged cultivation (to 168 h) depended on the growth phase of bacteria, and it was about 51.5% for IQ and at about 11.2% for MeIQx. Non-growing cells (in phosphate buffer), could reduce the content of IQ and PhIP from 72 h to the end of incubation. L. casei DN 114001 reduced genotoxicity of HCA (IQ from 46 to 48%; MeIQx from 35 to 65% and PhIP from 32 to 81%), and the degree depended on the incubation time, cell growth and the medium used. It may suggest that bacteria can metabolise or adsorb HCA.

  14. Application of regenerated DN-3100 Catalyst in hydrotreating unit%再生后DN-3100催化剂在加氢精制装置的应用

    Institute of Scientific and Technical Information of China (English)

    吴文涛; 李江松; 曹阳

    2011-01-01

    中国石油天然气股份有限公司辽阳石化分公司炼油厂为生产满足欧Ⅳ排放标准的精制柴油,采用再生后DN-3100催化剂和新鲜FH-UDS催化剂混合装填的方案,经催化剂干燥和预硫化后,用于新建的2.00 Mt/a汽柴油加氧精制装置.应用结果表明,在反应器入口温度为311℃,氢油体积比为381,体积空速为1.29 h-1操作条件下,精制柴油的硫质量分数为33 μg/g,满足欧Ⅳ柴油质量标准.%To produce refined diesel satisfying Europe Ⅳ emission standards, the mixed regenerated DN-3100 hydrotreating catalyst and fresh FH-UDS hydrotreating catalyst were applied in a new 2.0 MM TPY gaso line and diesel hydrotreating unit in the refinery of PetroChina liaoyang Petrochemical Company. The results showed that the sulfur in the refined diesel is 33 ppm which meets the Europe IV specifications at the condi tions of the reactor inlet temperature 311 ℃, hydrogen to oil volumetric ratio 381 and volumetric space velocity 1.29 h-1.

  15. Postnatal development of numbers and mean sizes of pancreatic islets and beta-cells in healthy mice and GIPR(dn transgenic diabetic mice.

    Directory of Open Access Journals (Sweden)

    Nadja Herbach

    Full Text Available The aim of this study was to examine postnatal islet and beta-cell expansion in healthy female control mice and its disturbances in diabetic GIPR(dn transgenic mice, which exhibit an early reduction of beta-cell mass. Pancreata of female control and GIPR(dn transgenic mice, aged 10, 45, 90 and 180 days were examined, using state-of-the-art quantitative-stereological methods. Total islet and beta-cell volumes, as well as their absolute numbers increased significantly until 90 days in control mice, and remained stable thereafter. The mean islet volumes of controls also increased slightly but significantly between 10 and 45 days of age, and then remained stable until 180 days. The total volume of isolated beta-cells, an indicator of islet neogenesis, and the number of proliferating (BrdU-positive islet cells were highest in 10-day-old controls and declined significantly between 10 and 45 days. In GIPR(dn transgenic mice, the numbers of islets and beta-cells were significantly reduced from 10 days of age onwards vs. controls, and no postnatal expansion of total islet and beta-cell volumes occurred due to a reduction in islet neogenesis whereas early islet-cell proliferation and apoptosis were unchanged as compared to control mice. Insulin secretion in response to pharmacological doses of GIP was preserved in GIPR(dn transgenic mice, and serum insulin to pancreatic insulin content in response to GLP-1 and arginine was significantly higher in GIPR(dn transgenic mice vs. controls. We could show that the increase in islet number is mainly responsible for expansion of islet and beta-cell mass in healthy control mice. GIPR(dn transgenic mice show a disturbed expansion of the endocrine pancreas, due to perturbed islet neogenesis.

  16. Increased TCR signal strength in DN thymocytes promotes development of gut TCRαβ((+))CD8αα((+)) intraepithelial lymphocytes.

    Science.gov (United States)

    Grandjean, Capucine L; Sumaria, Nital; Martin, Stefania; Pennington, Daniel J

    2017-09-06

    CD4((+))CD8((+)) "double positive" (DP) thymocytes differentiate into diverse αβ T cell sub-types using mechanistically distinct programs. For example, conventional αβ T cells develop from DP cells after partial-agonist T cell receptor (TCR) interactions with self-peptide/MHC, whereas unconventional αβ T cells, such as TCRαβ((+))CD8αα((+)) intraepithelial lymphocytes (IELs), require full-agonist TCR interactions. Despite this, DP cells appear homogeneous, and it remains unclear how distinct TCR signalling instructs distinct developmental outcomes. Moreover, whether TCR signals at earlier stages of development, for example in CD4((-))CD8((-)) double negative (DN) cells, impact on later fate decisions is presently unknown. Here, we assess four strains of mice that display altered TCR signal strength in DN cells, which correlates with altered generation of unconventional TCRαβ((+))CD8αα((+)) IELs. FVB/n mice (compared to C57BL/6 animals) and mice with altered preTCRα (pTα) expression, both displayed weaker TCR signalling in DN cells, an inefficient DN-to-DP transition, and reduced contribution of TCRαβ((+))CD8αα((+)) IELs to gut epithelium. Conversely, TCRαβ((+))CD8αα((+)) IEL development was favoured in mice with increased TCR signal strength in DN cells. Collectively, these data suggest TCR signal strength in DN cells directly impacts on subsequent DP cell differentiation, fundamentally altering the potential of thymocyte progenitors to adopt conventional versus unconventional T cell fates.

  17. Postnatal development of numbers and mean sizes of pancreatic islets and beta-cells in healthy mice and GIPR(dn) transgenic diabetic mice.

    Science.gov (United States)

    Herbach, Nadja; Bergmayr, Martina; Göke, Burkhard; Wolf, Eckhard; Wanke, Ruediger

    2011-01-01

    The aim of this study was to examine postnatal islet and beta-cell expansion in healthy female control mice and its disturbances in diabetic GIPR(dn) transgenic mice, which exhibit an early reduction of beta-cell mass. Pancreata of female control and GIPR(dn) transgenic mice, aged 10, 45, 90 and 180 days were examined, using state-of-the-art quantitative-stereological methods. Total islet and beta-cell volumes, as well as their absolute numbers increased significantly until 90 days in control mice, and remained stable thereafter. The mean islet volumes of controls also increased slightly but significantly between 10 and 45 days of age, and then remained stable until 180 days. The total volume of isolated beta-cells, an indicator of islet neogenesis, and the number of proliferating (BrdU-positive) islet cells were highest in 10-day-old controls and declined significantly between 10 and 45 days. In GIPR(dn) transgenic mice, the numbers of islets and beta-cells were significantly reduced from 10 days of age onwards vs. controls, and no postnatal expansion of total islet and beta-cell volumes occurred due to a reduction in islet neogenesis whereas early islet-cell proliferation and apoptosis were unchanged as compared to control mice. Insulin secretion in response to pharmacological doses of GIP was preserved in GIPR(dn) transgenic mice, and serum insulin to pancreatic insulin content in response to GLP-1 and arginine was significantly higher in GIPR(dn) transgenic mice vs. controls. We could show that the increase in islet number is mainly responsible for expansion of islet and beta-cell mass in healthy control mice. GIPR(dn) transgenic mice show a disturbed expansion of the endocrine pancreas, due to perturbed islet neogenesis.

  18. Overexpression of an Orchid (Dendrobium nobile) SOC1/TM3-Like Ortholog, DnAGL19, in Arabidopsis Regulates HOS1-FT Expression.

    Science.gov (United States)

    Liu, Xiao-Ru; Pan, Ting; Liang, Wei-Qi; Gao, Lan; Wang, Xiao-Jing; Li, Hong-Qing; Liang, Shan

    2016-01-01

    Flowering in the appropriate season is critical for successful reproduction in angiosperms. The orchid species, Dendrobium nobile, requires vernalization to achieve flowering in the spring, but the underlying regulatory network has not been identified to date. The MADS-box transcription factor DnAGL19 was previously identified in a study of low-temperature treated D. nobile buds and was suggested to regulate vernalization-induced flowering. In this study, phylogenetic analysis of DnAGL9 and the MADS-box containing proteins showed that DnAGL19 is phylogenetically closely related to the SOC1-like protein from orchid Dendrobium Chao Parya Smile, DOSOC1. The orchid clade closed to but is not included into the SOC1-1/TM3 clades associated with either eudicots or monocots, suggesting that DnAGL19 is an SOC1-1/TM3-like ortholog. DnAGL19 was found to be highly expressed in pseudobulbs, leaves, roots, and axillary buds but rarely in flowers, and to be substantially upregulated in axillary buds by prolonged low-temperature treatments. Overexpression of DnAGL19 in Arabidopsis thaliana resulted in a small but significantly reduced time to bolting, suggesting that flowering time was slightly accelerated under normal growth conditions. Consistent with this, the A. thaliana APETELA1 (AP1) gene was expressed at an earlier stage in transgenic lines than in wild type plants, while the FLOWERING LOCUS T (FT) gene was suppressed, suggesting that altered regulations on these transcription factors caused the weak promotion of flowering. HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 (HOS1) was slightly activated under the same conditions, suggesting that the HOS1-FT module may be involved in the DnAGL19-related network. Under vernalization conditions, FT expression was significantly upregulated, whereas HOS1 expression in the transgenic A. thaliana has a level similar to that in wild type. Taken together, these results suggest that DnAGL19 controls the action of the HOS1-FT module

  19. Overexpression of an orchid (Dendrobium nobile SOC1/TM3-like ortholog, DnAGL19, in Arabidopsis regulates HOS1-FT expression

    Directory of Open Access Journals (Sweden)

    Xiao-ru eLiu

    2016-02-01

    Full Text Available Flowering in the appropriate season is critical for successful reproduction in angiosperms. The orchid species, Dendrobium nobile, requires vernalization to achieve flowering in the spring, but the underlying regulatory network has not been identified to date. The MADS-box transcription factor DnAGL19 was previously identified in a study of low-temperature treated D. nobile buds and was suggested to regulate vernalization-induced flowering. In this study, phylogenetic analysis of DnAGL9 and the MADS-box containing proteins showed that DnAGL19 is phylogenetically closely related to the SOC1-like protein from orchid Dendrobium Chao Parya Smile, DOSOC1. The orchid clade closed to but is not included into the SOC1-1/TM3 clades associated with either eudicots or monocots, suggesting that DnAGL19 is an SOC1-1/TM3-like ortholog. DnAGL19 was found to be highly expressed in pseudobulbs, leaves, roots and axillary buds but rarely in flowers, and to be substantially upregulated in axillary buds by prolonged low-temperature treatments. Overexpression of DnAGL19 in Arabidopsis thaliana resulted in a small but significantly reduced time to bolting, suggesting that flowering time was slightly accelerated under normal growth conditions. Consistent with this, the A. thaliana APETELA1 (AP1 gene was expressed at an earlier stage in transgenic lines than in wild type plants, while the FLOWERING LOCUS T (FT gene was suppressed, suggesting that altered regulations on these transcription factors caused the weak promotion of flowering. HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 1 (HOS1 was slightly activated under the same conditions, suggesting that the HOS1-FT module may be involved in the DnAGL19-related network. Under vernalization conditions, FT expression was significantly upregulated, whereas HOS1 expression in the transgenic A. thaliana has a level similar to that in wild type. Taken together, these results suggest that DnAGL19 controls the action of the

  20. Ultrastructural features of skeletal muscle in adult and aging Ts65Dn mice, a murine model of Down syndrome.

    Science.gov (United States)

    Cisterna, Barbara; Costanzo, Manuela; Scherini, Elda; Zancanaro, Carlo; Malatesta, Manuela

    2013-10-01

    Patients with Down syndrome (DS) suffer from muscle hypotonia and an altered motor coordination whose basic mechanisms are still largely unknown. Interestingly, they show muscle weakness like healthy aged subjects, suggesting possible similarity with sarcopenia: to test this hypothesis, the Ts65Dn mouse, a suitable animal model of DS, was employed. The fine structure of skeletal fibres of the quadriceps femoris muscle was analysed in adult (12 months) and aging (19 months) animals and their age-matched euploid controls by combining morphometry and immunocytochemistry at transmission electron microscopy. Results demonstrated structural alterations of mitochondria and myonuclei reminiscent of those observed in age-related sarcopenia, supporting the hypothesis that trisomy leads to an early aging of skeletal muscle consistent with the multi-systemic premature aging typical of DS.

  1. An AC phase measuring interferometer for measuring dn/dT of fused silica and calcium fluoride at 193 nm

    Energy Technology Data Exchange (ETDEWEB)

    Shagam, R.N.

    1998-09-01

    A novel method for the measurement of the change in index of refraction vs. temperature (dn/dT) of fused silica and calcium fluoride at the 193 nm wavelength has been developed in support of thermal modeling efforts for the development of 193 nm-based photolithographic exposure tools. The method, based upon grating lateral shear interferometry, uses a transmissive linear grating to divide a 193 nm laser beam into several beam paths by diffraction which propagate through separate identical material samples. One diffracted order passing through one sample overlaps the undiffracted beam from a second sample and forms interference fringes dependent upon the optical path difference between the two samples. Optical phase delay due to an index change from heating one of the samples causes the interference fringes to change sinusoidally with phase. The interferometer also makes use of AC phase measurement techniques through lateral translation of the grating. Results for several samples of fused silica and calcium fluoride are demonstrated.

  2. First in situ determination of gas transport coefficients (DO2, DAr and DN2) from bulk gas concentration measurements (O2, N2, Ar) in natural sea ice

    DEFF Research Database (Denmark)

    Crabeck, O.; Delille, B.; Rysgaard, Søren

    2014-01-01

    evolution of an internal gas peak within the ice, we deduced the bulk gas transport coefficients for oxygen (DO2), argon (DAr), and nitrogen (DN2). The values fit to the few existing estimates from experimental work, and are close to the diffusivity values in water (1025 cm2 s21). We suggest that gas...

  3. Reliable dn/dc Values of Cellulose, Chitin, and Cellulose Triacetate Dissolved in LiCl/N,N-Dimethylacetamide for Molecular Mass Analysis.

    Science.gov (United States)

    Ono, Yuko; Ishida, Takashi; Soeta, Hiroto; Saito, Tsuguyuki; Isogai, Akira

    2016-01-11

    Freeze-dried microfibrillated cellulose (MFC) was directly dissolved in 8.0% w/w lithium chloride/N,N-dimethylacetamide (LiCl/DMAc), and MFC/LiCl/DMAc solutions with accurate MFC concentrations were prepared. The different MFC solutions were diluted to 1.0% and 0.5% w/v LiCl/DMAc, and subjected to size-exclusion chromatography with multiangle laser-light scattering and refractive index analyses (SEC/MALLS/RI), and off-line RI analysis to determine their refractive index increments (dn/dc). Chitin, cellulose triacetate, a poly(styrene) standard, and cellobiose were used for comparison. Each of the two determination methods gave different dn/dc values for MFC and chitin but similar dn/dc values for cellulose triacetate and poly(styrene). The anomalously small dn/dc values of MFC and chitin were explainable in terms of stable cellulose-LiCl and chitin-LiCl structures (i.e., formation of apparent covalent bonds between hydroxyl groups and LiCl) in the solutions. Thus, the SEC/MALLS/RI method provides reliable molecular mass parameters for cellulose and chitin.

  4. INGENIERIA METABOLICA PARA INCREMENTAR EL FLUX Y RENDIMIENTO DE ETANOL EN Escherichia coli ETANOLOGENICA

    OpenAIRE

    G. Huerta-Beristain; J. Utrilla-Carreri; G. Hernández-Chávez; Bolívar, F; Gosset, G; A. Martínez

    2005-01-01

    Se estudió el efecto de sobreexpresar genes del metabolismo de carbono sobre la velocidad específica (flux) de formación de etanol, en la cepa de Escherichia coli etanologénica KO11, la cual tiene integrado en el cromosoma los genes que codifican para las enzimas piruvato decarboxilasa (PdcZm) y la alcohol deshidrogenasa (AdhBZm) de Zymomonas mobilis. Los resultados muestran que en la cepa KO11, el control del flux de carbono está parcialmente fuera de la glucólisis y existe una actividad lim...

  5. Purification and Biochemical Characteristics of Cyanide-degrading Enzyme from Alcaligenes sp.DN25%产碱杆菌DN25中降氰酶的分离纯化及生化特性

    Institute of Scientific and Technical Information of China (English)

    汪艳华; 刘幽燕; 唐爱星; 李青云; 王顺成

    2012-01-01

    The cyanide-degrading enzyme from Alcaligenes sp. DN25 was purified through ultrafiltration, precipitation with 30 mg/mL protamine sulfate, 30%~70% fractional ammonium sulphate precipitation and hydrophobic chromatography on Phenyl-Toyopearl 650M, and the pure enzyme with the specific activity of 44 U/mg was obtained. After the proper reaction conditions including enzyme concentration and reaction time were determined for the cyanide-degrading activity assay, the purified enzyme properties were then studied in order to provide a theoretical basis for the future researches on cyanide-degradation mechanism and genetic engineering of strain DN2S. The results showed that the optimal pH and temperature were 8.0 and 30℃, respectively. Good stability of the enzyme was observed at pH 7.6-8.0 and its activity decreased quickly when pH reached up to 9.0. The activity of the purified enzyme could keep stable when preserved at 30 t for 10 h while fast deactivation happened at 60 CC. However, with glycine added, the enzyme activity still remained 19.6% after incubated at 60 ℃ for 20 min. The degradation of cyanide by the purified enzyme followed a typical Michaelis-Menten kinetics, with Km of 3.11 mmol/L and Vmax of 0.23 mmol L-1 min-1. Fig 11, Tab 1, Ref 23%以一株可降氰的产碱杆菌DN25为酶来源,通过超滤、30 mg/mL硫酸鱼精蛋白沉淀、30%~70%硫酸铵盐析和Phenyl-Toyopearl 650M疏水层析等步骤,获得比活力为44 U/mg的纯化酶制剂.在确定酶浓度、反应时间等氰降解活力测定条件后开展酶学性质研究,试图为将来氰降解代谢机理的深入研究和菌株的基因工程改造提供理论基础.研究结果表明,此纯化酶催化氰化物水解的最适pH值为8.0,最适温度为30℃.该酶在pH 7.0~8.0区域稳定,而在pH>9时会很快失活;在30℃保存10 h,酶活力保持稳定,高于60℃,酶快速失活.加入甘氨酸稳定剂,在60℃下保存20 min酶活仍可保留19.6%.酶促反应动

  6. Biodegradation of Cyanide by Immobilized Alcaligenes sp.DN25 Corncob as the Carrier%玉米芯固定化产碱杆菌DN25处理含氰废水研究

    Institute of Scientific and Technical Information of China (English)

    贺莉丽; 刘幽燕; 李青云; 徐少滨

    2011-01-01

    [目的]研究使用玉米芯作为固定化载体处理氰化物.[方法]以玉米芯为载体,对一株产碱杆菌DN25进行固定化,以实验室配制的模拟含氰废水为底物进行降解试验.[结果]采用菌体附着载体生长的方式来制备固定化细胞,最佳培养条件为温度30℃,pH8.0.固定化细胞对50 mg/L含氰废水45 min的降解率为98.5%,对300 mg/L含氰废水降解率可达91.9%.批式重复使用固定化细胞进行降解,前5次对氰化物的降解率仍可维持在80%以上.所设计的固定床反应器中系统运行稳定,固定化细胞降解效果良好,连续运行25d,前15 d对于氰化物的最大降解率高达99.25%.[结论]与其他传统载体相比,玉米芯为天然绿色植物,廉价易得,可降解,本身不会对环境造成二次污染,且玉米芯多孔、吸附能力强、固定化效果好.%[Objective] The aim was to study biodegradation of cyanide by immobilized Akaligenes sp. DN25 corncob as the carrier. [ Method ] The treatment of cyanide-containing wastewater by the immobilized Akaligenes sp. DN25 with the corncob as carrier was studied. [ Result ] The immobilization could be realized through the growth of the cell on the corncob during the culture process and the optimal culture condition was found to be 30 ℃ , pH 8.0. The degradation rate of 50 mg/L cyanide by the immobilized cell might achieve 98. 5% at 45 min, while the conversion of 91.9% achieved for 300 mg/L. The degradation of cyanide by the corncob-immobilized cell was carried out in the batch. The immobilized cell could be used repeated and the degradation rate of cyanide could maintain above 80% during the first five circle. When the immobilized cell was employed in a continuous-bioreactor for 25 days, it showed a good operation stability and degradation ability. The highest degradation rate could reach 99.25% during the first fifteen days. [ Conclusion] Compared with other traditional carrier, corncob, a natural green plant

  7. Survival of Bifidobacterium animalis DN-173 010 in the faecal microbiota after administration in lyophilised form or in fermented product - a randomised study in healthy adults.

    Science.gov (United States)

    Rochet, Violaine; Rigottier-Gois, Lionel; Ledaire, Audrey; Andrieux, Claude; Sutren, Malène; Rabot, Sylvie; Mogenet, Agnès; Bresson, Jean-Louis; Cools, Stéphanie; Picard, Céline; Goupil-Feuillerat, Nathalie; Doré, Joël

    2008-01-01

    The survival of Bifidobacterium animalis strain DN-173 010 was assessed after its ingestion in a fermented product or in a lyophilised form. Twelve healthy subjects were included in a randomised, open study with 2 parallel groups. The composition and activities of the faecal microbiota were monitored before (10-day baseline step), during (1-week product administration step) and after (10-day follow-up step) the ingestion of 1 of the 2 products. A colony immunoblotting method, fluorescent in situ hybridisation with group-specific DNA probes, and temporal temperature gradient gel electrophoresis using group-specific primers were carried out to compare survival of B. animalis strain DN-173 010 after ingestion of the 2 products, together with analyses of enzyme activities and faecal metabolites. At the end of the supplementation step, the mean number of B. animalis DN-173 010 quantified by immunodetection in the faeces of 5 of 6 subjects in each treatment group was >/=10(8) colony-forming units/g faeces. These numbers corresponded to an average survival of 22% for the lyophilised form and 20% for the fermented product. At the same step, the PCR temporal temperature gradient gel electrophoresis profiles showed a double band corresponding to the B. animalis DN-173 010 pattern for 11 subjects. No major modification was observed during the trial in either the dominant members of the faecal microbiota assessed by fluorescent in situ hybridisation or their activities. In conclusion, we show that the lyophilised form of B. animalis DN-173 010 survives transit and could represent a more convenient form to administer for long-term clinical trials.

  8. Incremento de la glucosa-6-fosfato-deshidrogenasa eritrocitaria en jóvenes con síndrome de Down tras un programa de actividad física de 12 semanas A 12-week physical activity program increases glucose-6-phosphate-dehydrogenase activity in Down syndrome adolescents

    Directory of Open Access Journals (Sweden)

    Francisco J. Ordóñez

    2005-12-01

    Full Text Available Recientemente se ha publicado que las células trisómicas presentan una mayor sensibilidad al daño oxidativo, que podría justificar la frecuente asociación de síndrome de Down a aterosclerosis, envejecimiento precoz, etc. Para conocer el posible papel de la actividad física moderada en la mejora de la capacidad antioxidante se estudió el comportamiento de la enzima glucosa-6-fosfato-deshidrogenasa (G6PDH eritrocitaria en 31 adolescentes varones (16.3 ± 1.1 años tras desarrollar un programa de 12 semanas con tres sesiones (45-60 minutos y una intensidad del 60-75% frecuencia cardíaca máxima teórica. Nuestros resultados indican una mayor actividad de G6PDH en individuos con síndrome de Down cuando se compara con controles sin trisomía ajustados a su sexo, edad e índice de masa corporal. Asimismo observamos un incremento significativo de su actividad tras completar nuestro programa de 12 semanas. Podemos concluir que la actividad física moderada mejora la capacidad antioxidante en jóvenes con síndrome de Down.In recent years it has been claimed that trisomic cells are more sensitive to oxidative stress since there is an imbalance in the hydrogen peroxide metabolism. We designed the present study to assess the activity level of antioxidant enzyme glucose-6-phosphate-dehydrogenase (G6PDH of erythrocytes in 31 male adolescents with Down syndrome (mean age 16.3 ± 1.1 after performing a 12 week aerobic training program. First of all, a significant increase of 14.9% in the catalytic activity of G6PDH was observed in male adolescents with Down syndrome when compared with age, sex and body mass-matched controls without trisomy. After 12-wk program its activity increased significantly compared to baseline value in Down syndrome individuals. Our data are consistent with previous evidence of the existence of higher oxidative stress in adolescents with Down syndrome when compared to the general population. We may also conclude that G6PDH

  9. Effect of yogurt containing Bifidobacterium animalis subsp . lactis DN-173010 probiotic on dental plaque and saliva in orthodontic patients.

    Science.gov (United States)

    Pinto, G S; Cenci, M S; Azevedo, M S; Epifanio, M; Jones, M H

    2014-01-01

    To assess how consumption of yogurt containing Bifidobacterium animalis subsp. lactis DN-173010 probiotic for a period of 2 weeks affects salivary and dental plaque levels of mutans streptococci and lactobacilli in patients undergoing orthodontic treatment. A crossover, double-blind, randomized and placebo-controlled clinical trial was performed with 26 volunteers. The study was divided into four periods. During periods 2 and 4, the volunteers ingested yogurt containing probiotic or control yogurt daily for 2 weeks. Periods 1 and 3 were a 1-week run-in period and 4-week washout period, respectively. Saliva and dental plaque samples were collected from each participant at the end of each period. Mutans streptococci, lactobacilli, and total cultivable microorganisms were counted. Values were compared between groups and across periods with the Wilcoxon's test. There was no difference between the yogurt containing probiotic and the control yogurt for any of the studied variables (all p > 0.05). A reduction in counts of total cultivable microorganisms was observed in dental plaque samples after ingestion of either yogurts (both p < 0.05 vs. baseline), but not in saliva (p < 0.05). Daily ingestion of yogurt with or without B. animalis subsp. lactis for a period of 2 weeks was beneficial in reducing total microbial counts in dental plaque. Therefore, no additional benefits were achieved by the use of the tested probiotic strain.

  10. High-resolution measurements of the exited states (n,pn), (n,dn) C-12 cross sections

    Science.gov (United States)

    Pillon, M.; Angelone, M.; Belloni, F.; Geerts, W.; Loreti, S.; Milocco, A.; Plompen, A. J. M.

    2017-09-01

    Measurements of C12 cross sections for the excited states (n,p0) up to (n,p4) and (n,d0), (n,d1) have been carried out. The Van de Graaff neutron generator of the EC-JRC-IRMM laboratory has been used for these measurements. A very thin tritiated target (263 μg/cm2) was employed with deuteron beams energies impinging on the target in the range 2.5-4.0 MeV. Neutrons in the range 18.9-20.7 MeV were produced with an intrinsic energy spread of 0.2-0.25% FWHM. With such narrow neutron energy spread, using a high energy resolution device such as a single crystal diamond detector, several peaks from the outgoing charged particles produced by the (n,pn), (n,dn) and also (n,α0) reactions appear in the pulse height spectrum. The peaks can be identified using the reaction Q-values. The diamond detector used for these measurements has shown an intrinsic energy resolution lower than 0.9% FWHM. The analysis of the peaks has permitted to derive the partial carbon reaction cross sections for several excited states. The results are presented in this paper with the associated uncertainties and they are compared with different versions of TENDL compilation when these data are available (e.g. versions 2009, 2010, 2011 and 2015) and also with experimental results available in the EXFOR database.

  11. Elucidation of the chemical and morphological structure of double-network (DN) hydrogels by high-resolution magic angle spinning (HRMAS) NMR spectroscopy.

    Science.gov (United States)

    Shestakova, Pavletta; Willem, Rudolph; Vassileva, Elena

    2011-12-23

    (1)H HRMAS NMR spectroscopy is applied to gain insight into the chemical and morphological structure of double-network (DN) hydrogels, prepared from poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (PAMPS) and poly(acrylamide) (PAAm). The method enables one to obtain detailed information at the molecular level about the formation of covalent bonds between the two polymer networks through non-reacted double bonds of the cross-linker N,N'-methylene bis(acrylamide) (MBAA). Evidence to the existence of strong hydrogen-bond interactions based on the N-H group of the PAMPS as a hydrogen-bond donor and the C=O group of the PAAm as a hydrogen-bond acceptor is also provided. These findings clarify the origin of the toughening mechanism and the exceptionally strong mechanical properties of DN gels, further supported by microhardness data. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. OISTER optical and near-infrared observations of the super-Chandrasekhar supernova candidate SN 2012dn: Dust emission from the circumstellar shell

    Science.gov (United States)

    Yamanaka, Masayuki; Maeda, Keiichi; Tanaka, Masaomi; Tominaga, Nozomu; Kawabata, Koji S.; Takaki, Katsutoshi; Kawabata, Miho; Nakaoka, Tatsuya; Ueno, Issei; Akitaya, Hiroshi; Nagayama, Takahiro; Takahashi, Jun; Honda, Satoshi; Omodaka, Toshihiro; Miyanoshita, Ryo; Nagao, Takashi; Watanabe, Makoto; Isogai, Mizuki; Arai, Akira; Itoh, Ryosuke; Ui, Takahiro; Uemura, Makoto; Yoshida, Michitoshi; Hanayama, Hidekazu; Kuroda, Daisuke; Ukita, Nobuharu; Yanagisawa, Kenshi; Izumiura, Hideyuki; Saito, Yoshihiko; Masumoto, Kazunari; Ono, Rikako; Noguchi, Ryo; Matsumoto, Katsura; Nogami, Daisaku; Morokuma, Tomoki; Oasa, Yumiko; Sekiguchi, Kazuhiro

    2016-10-01

    We present extensively dense observations of the super-Chandrasekhar supernova (SC SN) candidate SN 2012dn from -11 to +140 d after the date of its B-band maximum in the optical and near-infrared (NIR) wavelengths conducted through the OISTER ToO (Optical and Infrared Synergetic Telescopes for Education and Research Target of Opportunity) program. The NIR light curves and color evolutions up to 35 days after the B-band maximum provided an excellent match with those of another SC SN 2009dc, providing further support to the nature of SN 2012dn as an SC SN. We found that SN 2012dn exhibited strong excesses in the NIR wavelengths from 30 d after the B-band maximum. The H- and Ks-band light curves exhibited much later maximum dates at 40 and 70 d after the B-band maximum, respectively, compared with those of normal SNe Ia. The H- and Ks-band light curves subtracted by those of SN 2009dc displayed plateaued evolutions, indicating an NIR echo from the surrounding dust. The distance to the inner boundary of the dust shell is limited to 4.8-6.4 × 10-2 pc. No emission lines were found in its early phase spectra, suggesting that the ejecta-circumstellar material interaction could not occur. On the other hand, we found no signature that strongly supports the scenario of dust formation. The mass-loss rate of the pre-explosion system is estimated to be 10-6-10-5 M⊙ yr-1, assuming that the wind velocity of the system is 10-100 km s-1, which suggests that the progenitor of SN 2012dn could be a recurrent nova system. We conclude that the progenitor of this SC SN could be explained by the single-degenerate scenario.

  13. The Origin of the Near-infrared Excess in SN Ia 2012dn: Circumstellar Dust around the Super-Chandrasekhar Supernova Candidate

    Science.gov (United States)

    Nagao, Takashi; Maeda, Keiichi; Yamanaka, Masayuki

    2017-02-01

    The nature of progenitors of the so-called super-Chandrasekhar candidate Type Ia supernovae (SC-SNe Ia) has been actively debated. Recently, Yamanaka et al. reported a near-infrared (NIR) excess for SN 2012dn and proposed that the excess originates from an echo by circumstellar (CS) dust. In this paper, we examine a detailed distribution of the CS dust around SN 2012dn and investigate implications of the CS dust echo scenario for general cases of SC-SNe Ia. We find that a disk/bipolar CS medium configuration reproduces the NIR excess fairly well, where the radial density distribution is given by a stationary mass loss. The inner radius of the CS dust is 0.04 pc. The mass-loss rate of the progenitor system is estimated to be 1.2× {10}-5 and 3.2× {10}-6 M⊙ yr‑1 for the disk and bipolar CS medium configurations, respectively, which adds further support for the single-degenerate scenario. Our models limit SN 2009dc, another SC-SN Ia, to have a dust mass less than 0.16 times that of SN 2012dn. While this may merely indicate some variation on the CS environment among SC-SNe Ia, this could raise another interesting possibility. There could be two classes among SC-SNe Ia: the brighter SC-SNe Ia in a clean environment (SN 2009dc) and the fainter SC-SNe Ia in a dusty environment (SN 2012dn).

  14. The Scrambling Index of Primitive Digraphs Dn,q,s%本原有向图 Dn,q,s的 scrambling 指数

    Institute of Scientific and Technical Information of China (English)

    尤利华; 陈芳

    2013-01-01

    设n,q,s是正整数,满足1≤s<q≤n,g.c.d.(q,s)=1,且q+s≥n+1.定义有向图Dn,q,s =(V,E),其中V={1,2,⋯,n},E={(i,i+1)1≤i≤n-1}∪{(s,1),(n,n-q+1)}.显然,Dn,q,s是本原有向图,且是一类重要的极图.运用了数论和图论的方法得到了Dn,q,s的scrambling 指数,根据本原有向图D的本原指数exp ( D)与scrambling 指数k(D)的已知结论,猜想limn→∞ k(D)exp(D)=12.%Let n,q,s be integers,1≤sDn,q,s =(V, E), where V={1,2,⋯,n},E={(i,i+1)|1≤i≤n-1}∪{(s,1),(n,n-q+1)}.Obviously, Dn,q,s is a primitive digraph.In fact, Dn,q,s is an important extremal digraph.Scrambling index of Dn,q,s is studied by the method of Number theory and Graph theory .Moreover,based on the results of exponent exp ( D) and scrambling in-dex k( D) of the important primitive digraph D, conjecture lim n→∞k( D) exp( D) =1 2 is proposed .

  15. Longitudinal measures of cognition in the Ts65Dn mouse: Refining windows and defining modalities for therapeutic intervention in Down syndrome.

    Science.gov (United States)

    Olmos-Serrano, J Luis; Tyler, William A; Cabral, Howard J; Haydar, Tarik F

    2016-05-01

    Mouse models have provided insights into adult changes in learning and memory in Down syndrome, but an in-depth assessment of how these abnormalities develop over time has never been conducted. To address this shortcoming, we conducted a longitudinal behavioral study from birth until late adulthood in the Ts65Dn mouse model to measure the emergence and continuity of learning and memory deficits in individuals with a broad array of tests. Our results demonstrate for the first time that the pace at which neonatal and perinatal milestones are acquired is correlated with later cognitive performance as an adult. In addition, we find that life-long behavioral indexing stratifies mice within each genotype. Our expanded assessment reveals that diminished cognitive flexibility, as measured by reversal learning, is the most robust learning and memory impairment in both young and old Ts65Dn mice. Moreover, we find that reversal learning degrades with age and is therefore a useful biomarker for studying age-related decline in cognitive ability. Altogether, our results indicate that preclinical studies aiming to restore cognitive function in Ts65Dn should target both neonatal milestones and reversal learning in adulthood. Here we provide the quantitative framework for this type of approach.

  16. OISTER Optical and Near-Infrared Observations of the Super-Chandrasekhar Supernova Candidate SN 2012dn: Dust Emission from the Circumstellar Shell

    CERN Document Server

    Yamanaka, Masayuki; Tanaka, Masaomi; Tominaga, Nozomu; Kawabata, Koji S; Takaki, Katsutoshi; Kawabata, Miho; Nakaoka, Tatsuya; Ueno, Issei; Akitaya, Hiroshi; Nagayama, Takahiro; Takahashi, Jun; Honda, Satoshi; Omodaka, Toshihiro; Miyanoshita, Ryo; Nagao, Takashi; Watanabe, Makoto; Isogai, Mizuki; Arai, Akira; Itoh, Ryosuke; Ui, Takahiro; Uemura, Makoto; Yoshida, Michitoshi; Hanayama, Hidekazu; Kuroda, Daisuke; Ukita, Nobuharu; Yanagisawa, Kenshi; Izumiura, Hideyuki; Saito, Yoshihiko; Masumoto, Kazunari; Ono, Rikako; Noguchi, Ryo; Matsumoto, Katsura; Nogami, Daisaku; Morokuma, Tomoki; Oasa, Yumiko; Sekiguchi, Kazuhiro

    2016-01-01

    We present extensively dense observations of the super-Chandrasekhar supernova (SC SN) candidate SN 2012dn from $-11$ to $+140$ days after the date of its $B$-band maximum in the optical and near-infrared (NIR) wavelengths conducted through the OISTER ToO program. The NIR light curves and color evolutions up to 35 days after the $B$-band maximum provided an excellent match with those of another SC SN 2009dc, providing a further support to the nature of SN 2012dn as a SC SN. We found that SN 2012dn exhibited strong excesses in the NIR wavelengths from $30$ days after the $B$-band maximum. The $H$ and $K_{s}$-band light curves exhibited much later maximum dates at $40$ and $70$ days after the $B$-band maximum, respectively, compared with those of normal SNe Ia. The $H$ and $K_{s}$-band light curves subtracted by those of SN 2009dc displayed plateaued evolutions, indicating a NIR echo from the surrounding dust. The distance to the inner boundary of the dust shell is limited to be $4.8 - 6.4\\times10^{-2}$ pc. No ...

  17. Endurance and failure characteristics of main-shaft jet engine bearings at 3x10 to the 6th power DN

    Science.gov (United States)

    Bamberger, E. N.; Zaretsky, E. V.; Signer, H.

    1976-01-01

    Groups of thirty 120-mm bore angular contact ball bearings were endurance tested at a speed of 12,000 and 25,000 rpm and a thrust load of 66 721 N. The bearings were manufactured from a single heat of VIM-VAR AISI M-50 steel. At 1.44X1 million and 3.0x1 million DN, 84 483 and 74 800 bearing test hours were accumulated, respectively. Test results were compared with similar bearings made from CVM AISI M-50 steel run under the same conditions. Bearing lives at speeds of 3x1 million DN with the VIM-VAR AISI M-50 steel were nearly equivalent to those obtained at lower speeds. A combined processing and material life factor of 44 was found for VIM-VAR AISI M-50 steel. Continuous running after a spall has occurred at 3.0x1 million DN can result in a destructive fracture of the bearing inner race.

  18. Interferometric measurement of the temperature coefficient of the refractive index dn/dT and the coefficient of thermal expansion of Pr:YLF laser crystals.

    Science.gov (United States)

    Kazasidis, Orestis S; Wittrock, Ulrich

    2014-12-15

    We report interferometric measurements of the temperature coefficient of the refractive index (dn/dT) and the coefficient of thermal expansion (α) of a praseodymium-doped yttrium lithium fluoride (Pr:YLF) crystal and of a fused silica reference sample. Our phase-resolved interferometric method yields a large number of data points and thus allows a precise measurement and a good error estimation. Furthermore, both dn/dT and α are obtained simultaneously from a single measurement which reduces errors that can occur in separate measurements. Over the temperature range from 20 °C to 80 °C, the value of dn/dT of Pr:YLF decreases from −5.2 × 10(−6)/K to −6.2 × 10(−6)/K for the ordinary refractive index and from −7.6 × 10(−6)/K to −8.6 × 10(−6)/K for the extraordinary refractive index. The coefficient of thermal expansion for the a-axis of Pr:YLF increases from 16.4 × 10(−6)/K to 17.8 × 10(−6)/K over the same temperature range.

  19. Modulation of Lactobacillus casei in ileal and fecal samples from healthy volunteers after consumption of a fermented milk containing Lactobacillus casei DN-114 001Rif.

    Science.gov (United States)

    Rochet, Violaine; Rigottier-Gois, Lionel; Levenez, Florence; Cadiou, Julie; Marteau, Philippe; Bresson, Jean-Louis; Goupil-Feillerat, Nathalie; Doré, Joël

    2008-08-01

    Lactobacillus casei DN-114 001 is a probiotic strain able to interact with the immune system and to interfere with gastrointestinal pathogens. The derived strain DN-114 001Rif was studied during its transit through the upper and distal intestine of human volunteers. Seven volunteers participated in the study, which involved intestinal intubation to sample ileal contents and collection of fecal samples, with a wash-out period of 8 days between the 2 steps. The retrieval of the probiotic was analyzed in the ileum every 2 h for 8 h following the ingestion of one dose of the test product and in the feces prior to, during, and after daily consumption of the test product for 8 days. Persistence of the probiotic amplifiable DNA was assessed using temporal temperature gradient gel electrophoresis and real-time PCR. Fluorescent in situ hybridization allowed analysis of the composition of the dominant digestive microbiota. The ingestion of L. casei DN-114 001Rif led to a significant and transient increase of its amplifiable DNA in ileal and fecal samples. This is related to a high stability in the composition of dominant groups of the gut microbiota. Data from ileal samples are scarce and our study confirms the potentiality for interaction between probiotics and the human immune system.

  20. Inhibition of APP gamma-secretase restores Sonic Hedgehog signaling and neurogenesis in the Ts65Dn mouse model of Down syndrome.

    Science.gov (United States)

    Giacomini, Andrea; Stagni, Fiorenza; Trazzi, Stefania; Guidi, Sandra; Emili, Marco; Brigham, Elizabeth; Ciani, Elisabetta; Bartesaghi, Renata

    2015-10-01

    Neurogenesis impairment starting from early developmental stages is a key determinant of intellectual disability in Down syndrome (DS). Previous evidence provided a causal relationship between neurogenesis impairment and malfunctioning of the mitogenic Sonic Hedgehog (Shh) pathway. In particular, excessive levels of AICD (amyloid precursor protein intracellular domain), a cleavage product of the trisomic gene APP (amyloid precursor protein) up-regulate transcription of Ptch1 (Patched1), the Shh receptor that keeps the pathway repressed. Since AICD results from APP cleavage by γ-secretase, the goal of the current study was to establish whether treatment with a γ-secretase inhibitor normalizes AICD levels and restores neurogenesis in trisomic neural precursor cells. We found that treatment with a selective γ-secretase inhibitor (ELND006; ELN) restores proliferation in neurospheres derived from the subventricular zone (SVZ) of the Ts65Dn mouse model of DS. This effect was accompanied by reduction of AICD and Ptch1 levels and was prevented by inhibition of the Shh pathway with cyclopamine. Treatment of Ts65Dn mice with ELN in the postnatal period P3-P15 restored neurogenesis in the SVZ and hippocampus, hippocampal granule cell number and synapse development, indicating a positive impact of treatment on brain development. In addition, in the hippocampus of treated Ts65Dn mice there was a reduction in the expression levels of various genes that are transcriptionally regulated by AICD, including APP, its origin substrate. Inhibitors of γ-secretase are currently envisaged as tools for the cure of Alzheimer's disease because they lower βamyloid levels. Current results provide novel evidence that γ-secretase inhibitors may represent a strategy for the rescue of neurogenesis defects in DS.

  1. Detection and functional annotation of misregulated microRNAs in the brain of the Ts65Dn mouse model of Down syndrome

    Institute of Scientific and Technical Information of China (English)

    HE Xiang-jun; XIAO Yun; ZHANG Qi; MA Li-ping; LI Na; YANG Jing

    2013-01-01

    Background Brain hypoplasia and mental retardation in Down syndrome (DS) can be attributed to a severe and selective disruption of neurogenesis.Secondary disruption of the transcriptome,as well as primary gene dosage imbalance,is responsible for the phenotype.MicroRNA (miRNA) expression is relatively abundant in brain tissue.Perturbed miRNA expression might contribute to the cellular events underlying the pathology in DS.Methods MiRNA expression profiles in the cerebrum of Ts65Dn mice,a DS model,were examined with a real-time RT-PCR array.MiRNA target gene expression was detected by real-time quantitative PCR and Western blotting.Based on the prediction of their cerebrum-specific targets,the functions of the misregulated miRNAs were annotated by Gene Ontology (GO) enrichment analysis.Results A total of 342 miRNAs were examined.Among them,20 miRNAs showed decreased expression in the brains of Ts65Dn mice,and some of these belonged to the same family.Two known targets of the miR-200 family,Lfng and Zeb2,were specifically selected to compare their expression in the cerebrum of Ts65Dn mice with those of euploids.However,no significant difference was found in terms of mRNA and protein expression levels of these genes.By enrichment analysis of the cerebrum-specific targets of each miRNA,we found that 15 of the differential miRNAs could significantly affect target genes that were enriched in the GO biological processes related to nervous system development.Conclusion Perturbed expression of multiple functionally cooperative miRNAs contributes to the cellular events underlying the pathogenesis of DS.

  2. New structural and functional contexts of the Dx[DN]xDG linear motif: insights into evolution of calcium-binding proteins.

    Science.gov (United States)

    Rigden, Daniel J; Woodhead, Duncan D; Wong, Prudence W H; Galperin, Michael Y

    2011-01-01

    Binding of calcium ions (Ca²⁺) to proteins can have profound effects on their structure and function. Common roles of calcium binding include structure stabilization and regulation of activity. It is known that diverse families--EF-hands being one of at least twelve--use a Dx[DN]xDG linear motif to bind calcium in near-identical fashion. Here, four novel structural contexts for the motif are described. Existing experimental data for one of them, a thermophilic archaeal subtilisin, demonstrate for the first time a role for Dx[DN]xDG-bound calcium in protein folding. An integrin-like embedding of the motif in the blade of a β-propeller fold--here named the calcium blade--is discovered in structures of bacterial and fungal proteins. Furthermore, sensitive database searches suggest a common origin for the calcium blade in β-propeller structures of different sizes and a pan-kingdom distribution of these proteins. Factors favouring the multiple convergent evolution of the motif appear to include its general Asp-richness, the regular spacing of the Asp residues and the fact that change of Asp into Gly and vice versa can occur though a single nucleotide change. Among the known structural contexts for the Dx[DN]xDG motif, only the calcium blade and the EF-hand are currently found intracellularly in large numbers, perhaps because the higher extracellular concentration of Ca²⁺ allows for easier fixing of newly evolved motifs that have acquired useful functions. The analysis presented here will inform ongoing efforts toward prediction of similar calcium-binding motifs from sequence information alone.

  3. Effect of the consumption of a fermented dairy product containing Bifidobacterium lactis DN-173 010 on constipation in childhood: a multicentre randomised controlled trial (NTRTC: 1571).

    Science.gov (United States)

    Tabbers, Merit M; Chmielewska, Ania; Roseboom, Maaike G; Boudet, Claire; Perrin, Catherine; Szajewska, Hania; Benninga, Marc A

    2009-03-18

    Constipation is a frustrating symptom affecting 3% of children worldwide. Randomised controlled trials show that both polyethylene glycol and lactulose are effective in increasing defecation frequency in children with constipation. However, in 30-50%, these children reported abdominal pain, bloating, flatulence, diarrhoea, nausea and bad taste of the medication. Two recent studies have shown that the fermented dairy product containing Bifidobacterium lactis strain DN-173 010 is effective in increasing stool frequency in constipation-predominant irritable bowel syndrome patients with a defecation frequency DN-173 010 or a control product, twice a day, for 3 weeks. During the study all children are instructed to try to defecate on the toilet for 5-10 minutes after each meal (3 times a day) and daily complete a standardized bowel diary. Primary endpoint is stool frequency. Secondary endpoints are stool consistency, faecal incontinence frequency, pain during defecation, digestive symptoms (abdominal pain, flatulence), adverse effects (nausea, diarrhoea, bad taste) and intake of rescue medication (Bisacodyl). Rate of success and rate of responders are also evaluated, with success defined as > or = 3 bowel movements per week and or = 3 on the last week of product consumption. To demonstrate that the success percentage in the intervention group will be 35% and the success percentage in the control group (acidified milk without ferments, toilet training, bowel diary) will be 15%, with alpha 0.05 and power 80%, a total sample size of 160 patients was calculated. This study is aimed to show that the fermented dairy product containing Bifidobacterium lactis strain DN-173 010 is effective in increasing stool frequency after 3 weeks of product consumption in children with functional constipation and a defecation frequency < 3/week.

  4. Effects of probiotic Lactobacillus casei DN-114 001 in prevention of radiation-induced diarrhea: results from multicenter, randomized, placebo-controlled nutritional trial.

    Science.gov (United States)

    Giralt, Jordi; Regadera, José Perez; Verges, Ramona; Romero, Jesus; de la Fuente, Isabel; Biete, Albert; Villoria, Jesús; Cobo, Jose Maria; Guarner, Francisco

    2008-07-15

    To determine whether a probiotic drink containing Lactobacillus casei DN-114 001 reduces the incidence of radiation-induced diarrhea in patients with gynecologic cancer. Patients who were undergoing pelvic radiotherapy (45-50 Gy, conventional fractionation) for either cervical carcinoma (radiotherapy and weekly cisplatin) or endometrial adenocarcinoma (postoperative radiotherapy) were randomly assigned to a probiotic drink or placebo, in a double-blind fashion. The probiotic drink consisted of liquid yogurt containing L. casei DN-114 001 at 10(8) CFU/g. The patients recorded the daily the number of bowel movements and scored the stool consistency using the Bristol scale. Diarrhea was graded weekly according the Common Toxicity Criteria system. The primary endpoint was to reduce the incidence of diarrhea, defined by a Common Toxicity Criteria Grade of 2 or greater or the need for loperamide. A total of 85 patients were enrolled. Grade 2 or greater diarrhea and/or the use of loperamide was observed in 24 of 41 patients in the placebo group and 30 of 44 in the probiotic group (p = 0.568). No differences were found in the median time to the presentation of the primary endpoint. Probiotic intervention had a significant effect on stool consistency (p = 0.04). The median time for patients to present with Bristol scale stools of Type 6 or greater was 14 days for patients receiving the probiotic drink vs. 10 days for those receiving placebo. Nutritional intervention with the probiotic drink containing L. casei DN-114 001 does not reduce the incidence of radiation-induced diarrhea as defined by a Common Toxicity Criteria Grade 2 or greater. However, it had a significant effect on stool consistency as measured by the Bristol scale.

  5. Beta-amyloid overload does not directly correlate with SAPK/JNK activation and tau protein phosphorylation in the cerebellar cortex of Ts65Dn mice.

    Science.gov (United States)

    Lomoio, Selene; Scherini, Elda; Necchi, Daniela

    2009-11-10

    It is known that in the nervous tissue beta-amyloid overproduction and its extracellular or intracellular deposition can activate mitogen-activated protein kinases involved in tau protein phosphorylation. Hyperphosphorylated tau is not more able to bind neuron microtubules, leading to their disassembly and axon degeneration. We have previously described that at 10 months of age in the cerebellum of Ts65Dn mice, which are partially trisomic for the chromosome 16 and are considered a valuable model for Down syndrome, Purkinje cells undergo axon degeneration. Taking into consideration that Ts65Dn mice carry three copies of the gene encoding for the amyloid precursor protein, to characterize potential signaling events triggering the degenerative phenomenon, specific antibodies were used to examine the role of beta-amyloid overload in the activation of the stress activated kinase/c-jun N-terminal kinase (SAPK/JNK) and tau protein phosphorylation in the cerebellar cortex of 12-month-old Ts65Dn mice. We found small extracellular deposits of beta-amyloid at the borderline between the granule cell layer and the white matter, i.e., in the vicinity of the area where calbindin immunostaining of Purkinje cell axons revealed clusters of newly formed terminals of injured axons. Moreover, intracellular deposits were present in the somata of Purkinje cells. The level of activation of SAPK/JNK was greatly increased. The activation occurred in the "pinceaux" made by basket interneuron axons at the axon hillock of Purkinje cells. Antibody directed against tau protein phosphorylated at Ser-396/Ser-404 revealed positive NG2 cells and Bergman fibers in the molecular layer and oligodendrocytes in the white matter. Data indicate that beta-amyloid extracellular deposits could have exerted a local cytotoxic effect, leading to Purkinje cell axon degeneration. The activation of SAPK/JNK in basket cell "pinceaux" may be a consequence of altered functionality of Purkinje cells and may represent

  6. DN-3531/SDD-800/Z-3723催化剂在加氢改质装置上的工业应用

    Institute of Scientific and Technical Information of China (English)

    衣志成

    2016-01-01

    本文介绍了壳牌标准公司的催化剂DN-3531、SDD-800以及Z-3723在某石化公司加氢改质装置中的使用,包括催化剂装填、活化以及工业运转情况。运行和标定结果表明,通过调整操作条件,可成功地实现生产低凝柴油,达到了预期目标。

  7. The influence of Lactobacillus casei DN 114 001 on the activity of faecal enzymes and genotoxicity of faecal water in the presence of heterocyclic aromatic amines.

    Science.gov (United States)

    Nowak, Adriana; Śliżewska, Katarzyna; Błasiak, Janusz; Libudzisz, Zdzisława

    2014-12-01

    High activity of bacterial enzymes in human colon and genotoxicity of faecal water (FW) are biomarkers of the harmful action of microbiota. The aim of the present study was to assess the activity of β-glucuronidase and β-glucosidase and the genotoxicity of FW in vitro after incubation with 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline (IQ) or 2-amino-1-methyl-6-phenyl-1H-imidazo[4,5-b]pyridine (PhIP) and probiotic Lactobacillus casei DN 114 001 (Actimel). Our results indicate, that IQ and PhIP greatly increased the activity of faecal enzymes (it was up to four times higher, as measured by spectrophotometric methods) and the genotoxicity of FW (% DNA in the tail was up to 3.2 times higher, as evaluated by the comet assay on Caco-2 cells) in 15 individuals from three age-dependent groups (breast-fed children, adults aged 30-40 years, elderly aged 75-85 years). Lb. casei DN 114 001 decreased the activity of faecal enzymes and the genotoxicity of FW exposed to PhIP and IQ mostly to control values. The activity of faecal enzymes after incubation with IQ was reduced by 71.8% in the FW of children, 37.5% in adults and 64.2% in elderly (β-glucuronidase); as well as by 59.9% in children and 87.9% in elderly (β-glucosidase). For PhIP the reduction was by 59.0% in the FW of children, 50.0% in adults and 81.2% in elderly (β-glucuronidase) and by 20.2% in children, 20.7% in adults and 84.1% in elderly (β-glucosidase). Lb. casei DN 114 001 also decreased the genotoxicity of FW to the greatest extent in adults after incubation with IQ (by 65.4%) and PhIP (by 69.6%) and it was found to correlate positively with the decrease in faecal enzymes activity. In conclusion, Lb. casei DN 114 001 may exert the protective effects against genotoxic and possibly pro-carcinogenic effects of food processing-derived chemicals present in faecal water. Copyright © 2014. Published by Elsevier Ltd.

  8. Λ{sub b} → π{sup -}(D{sub s}{sup -})Λ{sub c}(2595), π{sup -}(D{sub s}{sup -})Λ{sub c}(2625) decays and DN, D*N molecular components

    Energy Technology Data Exchange (ETDEWEB)

    Liang, Wei-Hong [Guangxi Normal University, Department of Physics, Guilin (China); Centro Mixto Universidad de Valencia-CSIC Institutos de Investigacion de Paterna, Departamento de Fisica Teorica y IFIC, Valencia (Spain); Bayar, Melahat [Kocaeli University, Department of Physics, Izmit (Turkey); Oset, Eulogio [Centro Mixto Universidad de Valencia-CSIC Institutos de Investigacion de Paterna, Departamento de Fisica Teorica y IFIC, Valencia (Spain)

    2017-01-15

    From the perspective that Λ{sub c}(2595) and Λ{sub c}(2625) are dynamically generated resonances from the DN, D*N interaction and coupled channels, we have evaluated the rates for Λ{sub b} → π{sup -}Λ{sub c}(2595) and Λ{sub b} → π{sup -}Λ{sub c}(2625) up to a global unknown factor that allows us to calculate the ratio of rates and compare with experiment, where good agreement is found. Similarly, we can also make predictions for the ratio of rates of the, yet unknown, decays of Λ{sub b} → D{sub s}{sup -}Λ{sub c}(2595) and Λ{sub b} → D{sub s}{sup -}Λ{sub c}(2625) and make estimates for their individual branching fractions. (orig.)

  9. Novel angiogenic inhibitor DN-9693 that inhibits post-transcriptional induction of connective tissue growth factor (CTGF/CCN2) by vascular endothelial growth factor in human endothelial cells.

    Science.gov (United States)

    Kondo, Seiji; Tanaka, Noriko; Kubota, Satoshi; Mukudai, Yoshiki; Yosimichi, Gen; Sugahara, Toshio; Takigawa, Masaharu

    2006-01-01

    Connective tissue growth factor (CTGF/CCN2) is a potent angiogenic factor. In this report, we describe for the first time that vascular endothelial growth factor (VEGF)-mediated induction of the ctgf/ccn2 gene was a post-transcriptional event that was inhibited by a novel angiogenic inhibitor, DN-9693, in human umbilical vein endothelial cells. Steady-state mRNA levels of ctgf/ccn2 were remarkably increased by VEGF in a concentration-dependent manner, whereas the activity of the ctgf/ccn2 promoter was not responsive to VEGF as confirmed by a reporter gene assay and quantitative real-time PCR analysis. By employing a RNA degradation assay, we eventually found that the observed increase in the ctgf/ccn2 mRNA level was due to an increased stability of the mRNA induced by VEGF. DN-9693 at a dose of 0.1 to 2 ng/mL did not affect basal levels of ctgf/ccn2 mRNA; however, enhancement of ctgf/ccn2 mRNA expression by VEGF was specifically inhibited by DN-9693. Of importance, the inhibitory effects could be also ascribed to post-transcriptional regulation, because the VEGF-mediated increase in stability of ctgf/ccn2 mRNA was suppressed by DN-9693. Furthermore, we investigated the effects of DN-9693 on VEGF-induced activation of three subgroups of mitogen-activated protein kinase pathways and found that DN-9693 blocked the activation of these pathways by VEGF. These results suggest that VEGF increases ctgf/ccn2 mRNA stability through mitogen-activated protein kinase-mediated intracellular signaling cascade(s), which can be inhibited posttranscriptionally by a novel angiogenic inhibitor, DN-9693, in human umbilical vein endothelial cells.

  10. 含动物双歧杆菌DN-173 010的益生菌酸乳对便秘症状改善作用的研究%Effect of Probiotic Yogurt Containing Bifidobacterium animalis Strain DN-173 010 on Symptoms of Constipation

    Institute of Scientific and Technical Information of China (English)

    何梅; 胡刚; 魏洁; 杨月欣

    2009-01-01

    便秘对健康的危害不容忽视,但迄今尚无研究直接观察益生菌酸乳与便秘的关系.目的:观察含动物双歧杆菌DN-173 010的益生菌酸乳对便秘症状的改善作用.方法:159例成年便秘者随机分为试验组和对照组.试验组服用含DN-173 010的益生菌酸乳2周,每天100 g;对照组同法服用酸化牛奶2周.观察受试者试验前和试验期间的排便次数、排便状况、粪便性状以及饮食情况.结果:试验组服用含DN-173 010的益生菌酸乳后,每周排便次数、排便状况评分和粪便性状评分与自身试验前相比显著改善(P<0.01);与同时间点对照组相比,每周排便次数显著增加.排便状况和粪便性状评分显著降低(P=0.000).两组试验期间食物摄入量无明显差异.结论:服用含动物双歧杆菌DN-173 010的益生菌酸乳可明显改善成年便秘者的便秘症状.

  11. Activation of p53 in Down Syndrome and in the Ts65Dn Mouse Brain is Associated with a Pro-Apoptotic Phenotype.

    Science.gov (United States)

    Tramutola, Antonella; Pupo, Gilda; Di Domenico, Fabio; Barone, Eugenio; Arena, Andrea; Lanzillotta, Chiara; Broekaart, Diede; Blarzino, Carla; Head, Elizabeth; Butterfield, D Allan; Perluigi, Marzia

    2016-01-01

    Down syndrome (DS) is the most common genetic cause of intellectual disability, resulting from trisomy of chromosome 21. The main feature of DS neuropathology includes early onset of Alzheimer's disease (AD), with deposition of senile plaques and tangles. We hypothesized that apoptosis may be activated in the presence of AD neuropathology in DS, thus we measured proteins associated with upstream and downstream pathways of p53 in the frontal cortex from DS cases with and without AD pathology and from Ts65Dn mice, at different ages. We observed increased acetylation and phosphorylation of p53, coupled to reduced MDM2/p53 complex level and lower levels of SIRT1. Activation of p53 was associated with a number of targets (BAX, PARP1, caspase-3, p21, heat shock proteins, and PGC1α) that were modulated in both DS and DS/AD compared with age-matched controls. In particular, the most relevant changes (increased p-p53 and acetyl-p53 and reduced formation of MDM2/p53 complex) were found to be modified only in the presence of AD pathology in DS. In addition, a similar pattern of alterations in the p53 pathway was found in Ts65Dn mice. These results suggest that p53 may integrate different signals, which can result in a pro-apoptotic-phenotype contributing to AD neuropathology in people with DS.

  12. Photometric and spectroscopic monitoring of AA Tau, DN Tau, UX Tau A, T Tau, RY Tau, Lk Ca 4, and Lk Ca 7

    Science.gov (United States)

    Vrba, F. J.; Chugainov, P. F.; Weaver, W. B.; Stauffer, J. S.

    1993-01-01

    We report the results of a UBVRI photometric monitoring campaign for three classical T Tauri stars (AA Tau, DN Tau, and UX Tau A) and two weak emission line T Tauri stars (Lk Ca 4 and Lk Ca 7). Observations were obtained at three sites during a core observing period spanning UT 1985 October 14 through UT 1985 December 25, with additional observations continuing until UT 1986 April 6. Concurrent spectrophotometric observations were obtained for all main program stars except Lk Ca 7 and additionally for T Tau, RW Aur, and RY Tau. Periodic photometric variability, assumed to be the stars' rotation periods, were found for AA Tau, DN Tau, Lk Ca 4, and Lk Ca 7, respectively, as 8.2, 6.3, 3.4, and 5.7 days. Several U-filter flares were observed for Lk Ca 4 and Lk Ca 7, which are strongly concentrated toward phases of minimum light. Correlations are found between H-alpha line strengths and V magnitudes for AA Tau and RY Tau. An analysis of absolute color variations of classical T Tauri stars confirms that hot spots are the predominant cause of these stars' variability. Our overall results are consistent with earlier findings that long-lived cool spots are responsible for most of the variability found for weak-emission T Tauri stars, while temporal hot spots are primarily responsible for the observed variability found in classical T Tauri stars.

  13. Expression profile analysis of hippocampal CA1 pyramidal neurons in aged Ts65Dn mice, a model of Down syndrome (DS) and Alzheimer's disease (AD).

    Science.gov (United States)

    Alldred, Melissa J; Lee, Sang Han; Petkova, Eva; Ginsberg, Stephen D

    2015-09-01

    Down syndrome (DS) is caused by the triplication of human chromosome 21 (HSA21) and is the most common genetic cause of intellectual disability, with individuals having deficits in cognitive function including hippocampal learning and memory and neurodegeneration of cholinergic basal forebrain neurons, a pathological hallmark of Alzheimer's disease (AD). To date, the molecular underpinnings driving this pathology have not been elucidated. The Ts65Dn mouse is a segmental trisomy model of DS and like DS/AD pathology, displays age-related cognitive dysfunction and basal forebrain cholinergic neuron (BFCN) degeneration. To determine molecular and cellular changes important for elucidating mechanisms of neurodegeneration in DS/AD pathology, expression profiling studies were performed. Molecular fingerprinting of homogeneous populations of Cornu Ammonis 1 (CA1) pyramidal neurons was performed via laser capture microdissection followed by Terminal Continuation RNA amplification combined with custom-designed microarray analysis and subsequent validation of individual transcripts by qPCR and protein analysis via immunoblotting. Significant alterations were observed within CA1 pyramidal neurons of aged Ts65Dn mice compared to normal disomic (2N) littermates, notably in excitatory and inhibitory neurotransmission receptor families and neurotrophins, including brain-derived neurotrophic factor as well as several cognate neurotrophin receptors. Examining gene and protein expression levels after the onset of BFCN degeneration elucidated transcriptional and translational changes in neurons within a vulnerable circuit that may cause the AD-like pathology seen in DS as these individuals age, and provide rational targets for therapeutic interventions.

  14. Consumption of a fermented dairy product containing the probiotic Lactobacillus casei DN-114001 reduces the duration of respiratory infections in the elderly in a randomised controlled trial.

    Science.gov (United States)

    Guillemard, E; Tondu, F; Lacoin, F; Schrezenmeir, J

    2010-01-01

    Common infectious diseases (CID) of the airways and the gastrointestinal tract are still a considerable cause of morbidity and mortality in elderly. The present study examined the beneficial effect of a dairy product containing the probiotic strain Lactobacillus casei DN-114 001 (fermented product) on the resistance of free-living elderly to CID. The study was multicentric, double blind and controlled, involving 1072 volunteers (median age = 76.0 years) randomised for consumption of either 200 g/d of fermented (n 537) or control (non-fermented) dairy product (n 535) for 3 months, followed by an additional 1 month's follow-up. The results showed that, when considering all CID, the fermented product significantly reduced the average duration per episode of CID (6.5 v. 8 d in control group; P = 0.008) and the cumulative duration of CID (7 v. 8 d in control group; P = 0.009). Reduction in both episode and cumulative durations was also significant for all upper respiratory tract infections (URTI; P DN-114 001 in elderly was associated with a decreased duration of CID in comparison with the control group, especially for URTI such as rhinopharyngitis.

  15. A flavonoid agonist of the TrkB receptor for BDNF improves hippocampal neurogenesis and hippocampus-dependent memory in the Ts65Dn mouse model of DS.

    Science.gov (United States)

    Stagni, Fiorenza; Giacomini, Andrea; Guidi, Sandra; Emili, Marco; Uguagliati, Beatrice; Salvalai, Maria Elisa; Bortolotto, Valeria; Grilli, Mariagrazia; Rimondini, Roberto; Bartesaghi, Renata

    2017-09-04

    Intellectual disability is the unavoidable hallmark of Down syndrome (DS), with a heavy impact on public health. Reduced neurogenesis and impaired neuron maturation are considered major determinants of altered brain function in DS. Since the DS brain starts at a disadvantage, attempts to rescue neurogenesis and neuron maturation should take place as soon as possible. The brain-derived neurotrophic factor (BDNF) is a neurotrophin that plays a key role in brain development by specifically binding to tropomyosin-related kinase receptor B (TrkB). Systemic BDNF administration is impracticable because BDNF has a poor blood-brain barrier penetration. Recent screening of a chemical library has identified a flavone derivative, 7,8-dihydroxyflavone (7,8-DHF), a small-molecule that crosses the blood-brain barrier and binds with high affinity and specificity to the TrkB receptor. The therapeutic potential of TrkB agonists for neurogenesis improvement in DS has never been examined. The goal of our study was to establish whether it is possible to restore brain development in the Ts65Dn mouse model of DS by targeting the TrkB receptor with 7,8-DHF. Ts65Dn mice subcutaneously injected with 7,8-DHF in the neonatal period P3-P15 exhibited a large increase in the number of neural precursor cells in the dentate gyrus and restoration of granule cell number, density of dendritic spines and levels of the presynaptic protein synaptophysin. In order to establish the functional outcome of treatment, mice were treated with 7,8-DHF from P3 to adolescence (P45-50) and were tested with the Morris Water Maze. Treated Ts65Dn mice exhibited improvement of learning and memory, indicating that the recovery of the hippocampal anatomy translated into a functional rescue. Our study in a mouse model of DS provides novel evidence that treatment with 7,8-DHF during the early postnatal period restores the major trisomy-linked neurodevelopmental defects, suggesting that therapy with 7,8-DHF may represent a

  16. Effect of the consumption of a fermented dairy product containing Bifidobacterium lactis DN-173 010 on constipation in childhood: a multicentre randomised controlled trial (NTRTC: 1571

    Directory of Open Access Journals (Sweden)

    Perrin Catherine

    2009-03-01

    Full Text Available Abstract Background Constipation is a frustrating symptom affecting 3% of children worldwide. Randomised controlled trials show that both polyethylene glycol and lactulose are effective in increasing defecation frequency in children with constipation. However, in 30–50%, these children reported abdominal pain, bloating, flatulence, diarrhoea, nausea and bad taste of the medication. Two recent studies have shown that the fermented dairy product containing Bifidobacterium lactis strain DN-173 010 is effective in increasing stool frequency in constipation-predominant irritable bowel syndrome patients with a defecation frequency Methods/design It is a two nation (The Netherlands and Poland double-blind, placebo-controlled randomised multicentre trial in which 160 constipated children (age 3–16 years with a defecation frequency Bifidobacterium lactis DN-173 010 or a control product, twice a day, for 3 weeks. During the study all children are instructed to try to defecate on the toilet for 5–10 minutes after each meal (3 times a day and daily complete a standardized bowel diary. Primary endpoint is stool frequency. Secondary endpoints are stool consistency, faecal incontinence frequency, pain during defecation, digestive symptoms (abdominal pain, flatulence, adverse effects (nausea, diarrhoea, bad taste and intake of rescue medication (Bisacodyl. Rate of success and rate of responders are also evaluated, with success defined as ≥ 3 bowel movements per week and ≤1 faecal incontinence episode over the last 2 weeks of product consumption and responder defined as a subject reporting a stool frequency ≥ 3 on the last week of product consumption. To demonstrate that the success percentage in the intervention group will be 35% and the success percentage in the control group (acidified milk without ferments, toilet training, bowel diary will be 15%, with alpha 0.05 and power 80%, a total sample size of 160 patients was calculated. Conclusion This

  17. 空间蒙太奇 台湾鼎恩创意料理餐厅%MONTAGE TECHNIQUE INSPACE DESIGN DN INNNOVACION IN TAIWAN

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    空间表达的语言总是丰富多彩,正如设计师在台北的鼎恩创意料理(DN InnovaCion)餐厅中所运用的蒙太奇手法一般,他用艺术化的空间来突显餐厅所希望表现的独特味道。整个设计带有强烈的设计师个人风格,空间交错的流线美感、优雅流畅的线条、让人难忘的力度表达,都表现出高雅洁净的意境,

  18. $\\Lambda_b \\to \\pi^- (D_s^-) \\Lambda_c(2595),~\\pi^- (D_s^-) \\Lambda_c(2625)$ decays and $DN,~D^*N$ molecular components

    CERN Document Server

    Liang, Wei-Hong; Oset, E

    2016-01-01

    From the perspective that the $\\Lambda_c(2595)$ and $\\Lambda_c(2625)$ are dynamically generated resonances from the $DN,~D^*N$ interaction and coupled channels, we have evaluated the rates for $\\Lambda_b \\to \\pi^- \\Lambda_c(2595)$ and $\\Lambda_b \\to \\pi^- \\Lambda_c(2625)$ up to a global unknown factor that allows us to calculate the ratio of rates and compare with experiment, where good agreement is found. Similarly, we can also make predictions for the ratio of rates of the, yet unknown, decays of $\\Lambda_b \\to D_s^- \\Lambda_c(2595)$ and $\\Lambda_b \\to D_s^- \\Lambda_c(2625)$ and make estimates for their individual branching fractions.

  19. An atypical segment swap in the DN and DC domains of the Acr_tran family resistance-nodulation-cell division pump.

    Science.gov (United States)

    Acharya, Giriraj; Kaur, Gurmeet; Subramanian, Srikrishna

    2016-12-01

    Domain/segment swapping is an exchange of equivalent secondary structure element(s) among two or more protein domains resulting in the reconstitution of the original fold while simultaneously causing oligomerization. Here we report an example of the outer membrane factor docking region of the Acr_tran family (PF00873) resistance-nodulation-cell division pump, in which a swapped, misfolded state, of the ferredoxin-like fold of the DN and DC domains, effectuates oligomerization. The atypical segment swap and the associated displacement of a region of the ferredoxin-like fold leads to a topology that is distinct from the original fold. To our knowledge, such segment swaps and associated fold change are rare. This exemplifies the role of functional constraints including oligomerization that determine the interplay between sequence and the three-dimensional structure of proteins. Copyright © 2016 Elsevier Inc. All rights reserved.

  20. Rationale, Design, and Baseline Characteristics of ARTS-DN : A Randomized Study to Assess the Safety and Efficacy of Finerenone in Patients with Type 2 Diabetes Mellitus and a Clinical Diagnosis of Diabetic Nephropathy

    NARCIS (Netherlands)

    Ruilope, Luis M.; Agarwal, Rajiv; Chan, Juliana C.; Cooper, Mark E.; Gansevoort, Ron T.; Haller, Hermann; Remuzzi, Giuseppe; Rossing, Peter; Schmieder, Roland E.; Nowack, Christina; Ferreira, Anna C.; Pieper, Alexander; Kimmeskamp-Kirschbaum, Nina; Bakris, George L.

    2014-01-01

    Background/Aims: Finerenone decreases albuminuria in patients having heart failure with reduced ejection fraction and mild-to-moderate (stage 2-3) chronic kidney disease. The MinerAlocorticoid Receptor Antagonist Tolerability Study-Diabetic Nephropathy (ARTS-DN; NCT01874431) is a multicenter, random

  1. The structural innovative design of DN400 experimental facility with inversed U-shaped twin tower for uranium ion exchange%倒U型双塔式高效铀离子交换试验装置DN400结构上的创新设计

    Institute of Scientific and Technical Information of China (English)

    李庚; 雷泽勇; 刘清

    2012-01-01

    A DN400 experimental facility with inversed U-shaped twin towers for uranium ion exchange is designed and developed to solve the existing problems in ion exchange equipment, such as easy agglomeration on the upper layer of resin bed, much resin abrasion, high sensitiveness for mud and sand, and difficulty in producing ideal plug flow in back-wash process. The facility combined with the valve control principle,cross flow type sieve plate and the unique inlet and outlet structures can solve the above problems,and the efficiency of uranium extraction can be improved significantly.%倒U型双塔式高效铀吸附试验装置DN400的设计和研发,克服了目前离子交换设备在树脂床层上部易发生板结、树脂磨损大、对泥沙敏感度高、反冲操作无法形成理想的柱塞流的缺陷.该装置与阀门顺序控制、穿流式筛板、独特的进(出)液装置相结合,大大提高了铀的提取效率.

  2. Rationale, design, and baseline characteristics of ARTS-DN: a randomized study to assess the safety and efficacy of finerenone in patients with type 2 diabetes mellitus and a clinical diagnosis of diabetic nephropathy.

    Science.gov (United States)

    Ruilope, Luis M; Agarwal, Rajiv; Chan, Juliana C; Cooper, Mark E; Gansevoort, Ron T; Haller, Hermann; Remuzzi, Giuseppe; Rossing, Peter; Schmieder, Roland E; Nowack, Christina; Ferreira, Anna C; Pieper, Alexander; Kimmeskamp-Kirschbaum, Nina; Bakris, George L

    2014-01-01

    Finerenone decreases albuminuria in patients having heart failure with reduced ejection fraction and mild-to-moderate (stage 2-3) chronic kidney disease. The MinerAlocorticoid Receptor Antagonist Tolerability Study-Diabetic Nephropathy (ARTS-DN; NCT01874431) is a multicenter, randomized, double-blind, placebo-controlled, parallel-group, phase 2b study. ARTS-DN investigated whether the mineralocorticoid receptor antagonist finerenone reduces albuminuria without causing major alterations in serum potassium levels in patients with type 2 diabetes mellitus and a clinical diagnosis of DN who were receiving a renin-angiotensin-system (RAS) inhibitor. Patients were randomized to oral finerenone 1.25-20 mg or placebo once daily. The primary objectives were to assess the ratio of the urinary albumin-to-creatinine ratio at day 90 to that at baseline in patients receiving finerenone, and to compare it with that in the placebo group. Additional exploratory analyses included evaluating changes from baseline in serum potassium levels, efficacy and safety biomarkers, and health-related quality of life. Of 1,501 patients screened, 821 (the sample population) received at least one dose of finerenone/placebo. Baseline characteristics included: male, 77.8%; white, 84.2%; very high albuminuria (formerly macroalbuminuria), 38.4%; high albuminuria (formerly microalbuminuria), 60.3%; median (range) estimated glomerular filtration rate, 66.3 (24.5-130.7) ml/min/1.73 m(2); and systolic blood pressure (mean ± standard deviation), 138.1 ± 14.4 mm Hg. There was a history of cardiovascular disease in 39.6%, diabetic neuropathy in 20.0%, and diabetic retinopathy in 19.9% of patients. ARTS-DN is the first phase 2b trial of finerenone in combination with a RAS inhibitor in patients with type 2 diabetes mellitus and a clinical diagnosis of DN. © 2015 S. Karger AG, Basel.

  3. Specific IgA to lactic acid bacteria in feces of children consuming milk fermented by yoghurt symbiosis and Lactobacillus casei (Danone strain DN 114 001).

    Science.gov (United States)

    Faure, G C; Morisset, M; Gobert, B; Guérin, C; Pedone, C; Bouley, C; Béné, M C

    2001-01-01

    An immunoreactive role of lactic acid bacteria established in animals has seldom been investigated in humans. In a large-scale clinical study, children from day-care centers received either yoghurt (Y), milk fermented by yoghurt symbiosis and Lactobacillus casei (DN 114 001) (YC), or gelified milk (GM) as diet supplements during two 30-day supplementation periods separated by one 30-day period without supplementation. Feces samples were collected before, during, and after the 2nd supplementation period. Proteins were extracted in a buffer containing enzymatic inhibitors. IgA levels were assessed and adjusted to the weight of feces samples. Specific IgA to lactic acid bacteria strains (Streptococcus thermophilus 8901A, 8902A; Lactobacillus bulgaricus; Lactobacillus casei) present in Y and YC were assayed in ELISA and adjusted to individual IgA levels. Mean levels of fecal IgA were within reported ranges for pediatric populations of similar age. IgA levels decreased significantly but transiently in children receiving Y, and increased significantly in children receiving GM, but did not vary in the group of children who were given YC. Specific IgA to the 4 strains tested increased significantly during the supplementation period only in the group of children receiving GM, while it was transient and not significant in children receiving YC. No variation was noted in children given Y Specific IgA to lactic acid bacteria can be assayed in feces. Supplementation with fermented milks might induce a mucosal tolerance to environmental flora.

  4. Increased excitability and altered action potential waveform in cerebellar granule neurons of the Ts65Dn mouse model of Down syndrome.

    Science.gov (United States)

    Usowicz, Maria M; Garden, Claire L P

    2012-07-17

    Down syndrome (DS) is characterized by intellectual disability and impaired motor control. Lack of coordinated movement, poor balance, and unclear speech imply dysfunction of the cerebellum, which is known to be reduced in volume in DS. The principal cause of the smaller cerebellum is a diminished number of granule cells (GCs). These neurons form the 'input layer' of the cerebellar cortex, where sensorimotor information carried by incoming mossy fibers is transformed before it is conveyed to Purkinje cells and inhibitory interneurons. However, it is not known how processing of this information is affected in the hypogranular cerebellum that characterizes DS. Here we explore the possibility that the electrical properties of the surviving GCs are changed. We find that in the Ts65Dn mouse model of DS, GCs have a higher input resistance at voltages approaching the threshold for firing, which causes them to be more excitable. In addition, they fire narrower and larger amplitude action potentials. These subtly modified electrical properties may result in atypical transfer of information at the input layer of the cerebellum.

  5. Measuring the partial width of the 56Ni proton-capture resonance through (d,n) with VANDLE and MoNA-LISA

    Science.gov (United States)

    Peters, William; Grzywacz, R.; Madurga, M.; Paulauskas, S.; Taylor, S.; Allen, J.; Cizewski, J. A.; Manning, B.; Howard, M. E.; Bardayan, D. W.; Pain, S. D.; Clement, R. C. C.; Ilyushkin, S.; O'Malley, P. D.; Ikeyama, R.; Kozub, R. L.; Long, K. D.; Bergstrom, Z. J.; Deyoung, P. A.; Rogers, W. F.; Smith, J.; Jones, M.; Baumann, T.; Thoennessen, M.; Vandle; Mona Collaboration

    2013-10-01

    Due to the long half-life of 56Ni and the low proton threshold of 57Cu, the (p, γ) reaction on 56Ni is the linchpin reaction for heavier elements in the rp process of explosive nucleosynthesis. A new attempt to measure the proton partial width of the excited 1/2- state in 57Cu and to indirectly extract the proton-capture resonance strength was performed at the National Superconducting Cyclotron Laboratory using the Versatile Array of Neutron Detectors at Low Energy (VANDLE) along with the MoNA-LISA neutron array. The VANDLE setup of digitizing electronics was event-matched to the MoNA-LISA-Sweeper data acquisition system. After a test run with a stable 40Ca beam, a beam of 56Ni impinged onto a deuterated polyethylene target inducing (d,n) transfer reactions to predominantly single-proton states in 57Cu. These experiments were the commissioning experiments for VANDLE with a transfer reaction. Details of the unique experimental setup will be presented as well as current analysis of the data with both 40Ca and 56Ni beams. Work supported in part by the USDoE Office of Science and the National Nuclear Security Administration Stewardship Science Academic Alliance and the National Science Foundation.

  6. Lack of behavioral and cognitive effects of chronic ethosuximide and gabapentin treatment in the Ts65Dn mouse model of Down syndrome.

    Science.gov (United States)

    Vidal, V; García, S; Martínez, P; Corrales, A; Flórez, J; Rueda, N; Sharma, A; Martínez-Cué, C

    2012-09-18

    The Ts65Dn (TS) mouse model of Down syndrome (DS) displays a number of behavioral, neuromorphological and neurochemical phenotypes of the syndrome. Altered GABAergic transmission appears to contribute to the mechanisms responsible for the cognitive impairments in TS mice. Increased functional expression of the trisomic gene encoding an inwardly rectifying potassium channel, subfamily J, member 6 (KCNJ6) has been reported in DS and TS mice, along with the consequent impairment in GAB Aergic function. Partial display of DS phenotypes in mice harboring a single trisomy of Kcnj6 provides compelling evidence for a functional role of increased channel expression in some of the abnormal neurological phenotypes found in DS. Notably, the antiepileptic drug (AED) ethosuximide (ETH), but not other AEDs such as gabapentin (GAB), is known to inhibit KCNJ6 channels in mice. Here, we report the effect of chronic ETH and GAB on the behavioral and cognitive phenotypes of TS and disomic control (CO) mice. Neither drug significantly affected sensorimotor abilities, motor coordination or spontaneous activity in TS and CO mice. Also, ETH and GAB did not induce anxiety in the open field or plus maze tests, did not alter performance in the Morris water maze, and did not affect cued - or context - fear conditioning. Our results thus suggest that KCNJ6 may not be a promising drug target candidate in DS. As a corollary, they also show that long-term use of ETH and GAB is devoid of adverse behavioral and cognitive effects.

  7. On the Nature of Type Ia-CSM Supernovae: Optical and Near-Infrared Spectra of SN 2012ca and SN 2013dn

    CERN Document Server

    Fox, Ori D; Filippenko, Alexei V; Mauerhan, Jon; Becker, Juliette; Borish, H Jacob; Cenko, S Bradley; Clubb, Kelsey I; Graham, Melissa; Hsiao, Eric; Kelly, Patrick L; Lee, William H; Marion, G H; Milisavljevic, Dan; Parrent, Jerod; Shivvers, Isaac; Skrutskie, Michael; Smith, Nathan; Wilson, John; Zheng, Weikang

    2014-01-01

    A growing subset of Type Ia supernovae (SNe Ia) show evidence for unexpected interaction with a dense circumstellar medium (SNe Ia-CSM). The precise nature of the progenitor, however, remains debated owing to spectral ambiguities arising from a strong contribution from the CSM interaction. Late-time spectra offer potential insight if the post-shock cold, dense shell becomes sufficiently thin and/or the ejecta begin to cross the reverse shock. To date, few high-quality spectra of this kind exist. Here we report on the late-time optical and infrared spectra of the SNe~Ia-CSM 2012ca and 2013dn. These SNe Ia-CSM spectra exhibit low [Fe III]/[Fe II] ratios and strong [Ca II] at late epochs. Such characteristics are reminiscent of the super-Chandrasekhar-mass (SC) candidate SN 2009dc, for which these features suggested a low-ionisation state due to high densities, although the broad Fe features admittedly show similarities to the blue "quasi-continuum" observed in some core-collapse SNe Ibn and IIn. Neither SN 2012...

  8. The effect of DN (dominant-negative) Ku70 and reoxygenation on hypoxia cell-kill: evidence of hypoxia-induced potentially lethal damage.

    Science.gov (United States)

    Urano, Muneyasu; Li, Gloria C; He, Fuqiu; Minami, Akiko; Burgman, Paul; Ling, C Clifton

    2012-07-01

    To study the effect of DN (dominant-negative) Ku70 and reoxygenation on the hypoxia-induced cell-kill. Cell lines were human colorectal carcinoma HCT8 and HT29 cells and their respective derivatives, v-HCT8 and v-HT29 infected with DNKu70-containing adenovirus. Cells were plated in glass tubes and made hypoxic by flushing N(2) gas containing 0, 0.1 or 0.5% O(2). Cell survival was determined by colony formation assay immediately after 0-96 h hypoxia. To reoxygenate medium were replaced fresh following 48 or 72 h in hypoxia and cells were incubated in aerobic environment for 2-24 h before survival assay. When incubated in hypoxia, cells lost reproductive capability ∼ exponentially as a function of time in hypoxia, and depending on the O(2) concentration. DNKu70 rendered cells more prone to hypoxia-induced cell-kill. Following reoxygenation cell survival increased rapidly but without detectable cell proliferation during first 24 hours. This evinced hypoxia-induced potentially lethal damage (PLD) that was repairable upon reoxygenation. DNKu70 did not significantly inhibit this repair. Hypoxia-induced cell lethality was facilitated by DNKu70, but substantially repaired upon reoxygenation. This may have negative impact on the effect of reoxygenation in cancer therapy.

  9. Maitrise de la fermentation alcoolique sous stress éthanolique, thermique et osmotique de la souche Saccharomyces cerevisiae YS-DN1 en vue de la préparation du vinaigre de fruits

    Directory of Open Access Journals (Sweden)

    Majid MOUNIR

    2016-07-01

    Full Text Available The purpose of the present study is to isolate, identify and characterize new yeast species of industrial interest. A total of 54 strains were isolated and identified from agricultural raw products and from by-products of the food industry. Among these, four strains were selected, purified and tested for their ability to perform efficient fermentation on Bouslikhène date juice. After the sequencing of the 18S gene of the ribosomal DNA, the two strains YS-DN1 and YS-M isolated respectively from dates and molasses have been identified belonging to Saccharomyces cerevisiae species. In addition, the other two strains, YS-OMP and YS-G isolated from olives and grapes belonged to Kluyveromyces marxianus (78% homology and Candida utilis (89% homology species, respectively. In addition, YS-DN1 strain showed the highest performance in terms of ethanol production in comparison with two other industrial reference strains. YS-DN1 strain was the only one that was able to grow at a temperature ranged from 35 to 40°C in the presence of a high alcoholic concentration and aggressive osmotic pressure condition. Finally, production of the cellular biomass of YS-DN1 strain was improved by optimization of temperature, Brix and pH fixed respectively at 29.75°C, 15.7% and 4.15. These values allowed to obtain a maximum of cells of about 8.4x10 8 UFC/ml. It was concluded that this strain is well suited for fruit vinegar production at a large-scale.

  10. Neuropathic pain phenotyping as a predictor of treatment response in painful diabetic neuropathy: data from the randomized, double-blind, COMBO-DN study.

    Science.gov (United States)

    Bouhassira, Didier; Wilhelm, Stefan; Schacht, Alexander; Perrot, Serge; Kosek, Eva; Cruccu, Giorgio; Freynhagen, Rainer; Tesfaye, Solomon; Lledó, Alberto; Choy, Ernest; Marchettini, Paolo; Micó, Juan Antonio; Spaeth, Michael; Skljarevski, Vladimir; Tölle, Thomas

    2014-10-01

    Sensory profiles are heterogeneous in neuropathic pain disorders, and subgroups of patients respond differently to treatment. To further explore this, patients in the COMBO-DN study were prospectively assessed by the Neuropathic Pain Symptom Inventory (NPSI) at baseline, after initial 8-week therapy with either duloxetine or pregabalin, and after subsequent 8-week combination/high-dose therapy. Exploratory post hoc cluster analyses were performed to identify and characterize potential subgroups through their scores in the NPSI items. In patients not responding to initial 60 mg/d duloxetine, adding 300 mg/d pregabalin for combination treatment was particularly effective regarding the dimensions pressing pain and evoked pain, whereas maximizing the duloxetine dose to 120 mg/d appeared more beneficial regarding paresthesia/dysesthesia. In contrast, adding 60 mg/d duloxetine to 300 mg/d pregabalin in case of nonresponse to initial pregabalin led to numerically higher decreases in all NPSI dimensions/items compared to maximizing the pregabalin dose to 600 mg/d. Cluster analysis revealed 3 patient clusters (defined by baseline scores for the 10 NPSI sensory items) with different pain profiles, not only in terms of overall pain severity, but also across NPSI items. Mean Brief Pain Inventory average pain improved in all clusters during combination/high-dose therapy. However, in patients with severe pain, the treatment effect showed a trend in favor of high-dose monotherapy, whereas combination therapy appeared to be more beneficial in patients with moderate and mild pain (not significant). These complementary exploratory analyses further endorse the idea that sensory phenotyping might lead to a more stratified treatment and potentially to personalized pain therapy. Copyright © 2014 The Authors. Published by Elsevier B.V. All rights reserved.

  11. Enhanced biological phosphorus removal under alternating anaerobic and anoxic conditions. Pt. 2. The sorption-denitrification-P-removal-system; Vermehrte biologische Phosphorelimination durch alternierende anaerob/anoxische Milieubedingungen. T. 2. S-DN-P-Biofilmverfahren

    Energy Technology Data Exchange (ETDEWEB)

    Sieker, C.A. [Berliner Wasserbetriebe, Berlin (Germany); Hegemann, W. [Technische Univ. Berlin (Germany). Fachgebiet Siedlungswasserwirtschaft

    2000-07-01

    The Sorption-Denitrification-P-Removal-system (S-DN-P-system) is a process which combines biological excess P-removal and denitrification in a biofilm system. The accumulation of polyP organisms is achieved by sequencing anaerobic and anoxic conditions. The biofilm is in alternating contact with primary treated wastewater (anaerobic phase) and nitrified wastewater (anoxic phase). In the anaerobic phase, P-Release takes place and readily biodegradable organic substrate is fermented to volatile fatty acids and absorbed by the polyP organisms. In addition to this, other organic substrate is physically/chemically adsorbed in the biofilm. In the anoxic phase the biofilm dentirifies the ab/adsorbed organic substrate and, at the same time, P-uptake and polyP formation occurs. The research on the S-DN-P-system shows that P-removal in the anaerobic/anoxic system works well and ab- and adsorption mechanisms nearly remove all of the readily biodegradable substrate in the anaerobic phase. Consequently, this substrate can be used for denitrification in the anoxic phase. The S-DN-P-Process is a very promising concept. However, up to now the technical scale-up is unclear. This is subject to further investigation at the Technical University of Berlin. (orig.) [German] Das S-DN-P-Verfahren ist ein Biofilmverfahren, welches die vermehrte biologische Phosphorelemination und die Denitrifikation kombiniert. Die Anreicherung von denitrifizierenden polyP-Organismen wird durch alternierende anaerob-anoxische Milieubedingungen erreicht. Der Biofilm wird abwechselnd mit vorgeklaertem Abwasser beschickt (Sorptionsphase) und nitrifiziertem Abwasser (Denitrifikationsphase). In der anaeroben Sorptionsphase erfolgt eine P-Rueckloesung sowie die Aufnahme und zellinterne Speicherung von organischen Saeuren durch polyP-Organismen. Darueber hinaus werden andere organische Substanzen im Biofilm chemisch/physikalisch adsorbiert. In der Denitrifikationsphase werden die zuvor gespeicherten und

  12. AAV-dominant negative tumor necrosis factor (DN-TNF) gene transfer to the striatum does not rescue medium spiny neurons in the YAC128 mouse model of Huntington's disease.

    Science.gov (United States)

    Alto, Laura Taylor; Chen, Xi; Ruhn, Kelly A; Treviño, Isaac; Tansey, Malú G

    2014-01-01

    CNS inflammation is a hallmark of neurodegenerative disease, and recent studies suggest that the inflammatory response may contribute to neuronal demise. In particular, increased tumor necrosis factor (TNF) signaling is implicated in the pathology of both Parkinson's disease (PD) and Alzheimer's disease (AD). We have previously shown that localized gene delivery of dominant negative TNF to the degenerating brain region can limit pathology in animal models of PD and AD. TNF is upregulated in Huntington's disease (HD), like in PD and AD, but it is unknown whether TNF signaling contributes to neuronal degeneration in HD. We used in vivo gene delivery to test whether selective reduction of soluble TNF signaling could attenuate medium spiny neuron (MSN) degeneration in the YAC128 transgenic (TG) mouse model of Huntington's disease (HD). AAV vectors encoding cDNA for dominant-negative tumor necrosis factor (DN-TNF) or GFP (control) were injected into the striatum of young adult wild type WT and YAC128 TG mice and achieved 30-50% target coverage. Expression of dominant negative TNF protein was confirmed immunohistologically and biochemically and was maintained as mice aged to one year, but declined significantly over time. However, the extent of striatal DN-TNF gene transfer achieved in our studies was not sufficient to achieve robust effects on neuroinflammation, rescue degenerating MSNs or improve motor function in treated mice. Our findings suggest that alternative drug delivery strategies should be explored to determine whether greater target coverage by DN-TNF protein might afford some level of neuroprotection against HD-like pathology and/or that soluble TNF signaling may not be the primary driver of striatal neuroinflammation and MSN loss in YAC128 TG mice.

  13. DN-R175H p53 mutation is more effective than p53 interference in inducing epithelial disorganization and activation of proliferation signals in human carcinoma cells: role of E-cadherin.

    Science.gov (United States)

    Rieber, Manuel; Strasberg Rieber, Mary

    2009-10-01

    One of the hallmarks of carcinomas is epithelial disorganization, linked to overexpression of matrix metalloproteases (MMP) like MMP-9, loss of intercellular E-cadherin and activation of epidermal growth receptor (EGFR/erbB1). Since the p53 tumor suppressor pathway is inactivated in most human cancers due to gene mutations or defective wt p53 signaling, we now investigated in human wt p53 breast carcinoma MCF-7 cells, whether single treatment with the p53 transactivation pharmacological inhibitor pifithrin-alpha, transient p53 siRNA interference or stable insertion of a dominant-negative (DN) R175H p53 mutant increase: (i) EGFR/erbB1 activation, (ii) MMP-9 expression and (iii) loss of surface E-cadherin. Transient abrogation of wt p53 function increased phosphorylation of EGFR/erbB1 and MMP-9 expression. However, all these effects were much more pronounced in cells stably transduced with the dominant negative-Arg-175His mutant p53 (DN-R175H mutant p53), which also showed loss of epithelial cytoarchitecture and extensive E-cadherin downregulation. Collectively, these results support the notion that the DN-R175H mutant p53 exerts a gain of oncogenic function by promoting disruption of E-cadherin intercellular contacts and activation of proliferation signals. Our data suggests that epithelial shape and growth control are unequally affected depending on how wt p53 function is impaired and whether partial or full tumor suppressor activity is lost.

  14. Effect of Patiromer on Serum Potassium Level in Patients With Hyperkalemia and Diabetic Kidney Disease: The AMETHYST-DN Randomized Clinical Trial.

    Science.gov (United States)

    Bakris, George L; Pitt, Bertram; Weir, Matthew R; Freeman, Mason W; Mayo, Martha R; Garza, Dahlia; Stasiv, Yuri; Zawadzki, Rezi; Berman, Lance; Bushinsky, David A

    2015-07-14

    Hyperkalemia is a potentially life-threatening condition predominantly seen in patients treated with renin-angiotensin-aldosterone system (RAAS) inhibitors with stage 3 or greater chronic kidney disease (CKD) who may also have diabetes, heart failure, or both. To select starting doses for a phase 3 study and to evaluate the long-term safety and efficacy of a potassium-binding polymer, patiromer, in outpatients with hyperkalemia. Phase 2, multicenter, open-label, dose-ranging, randomized clinical trial (AMETHYST-DN), conducted at 48 sites in Europe from June 2011 to June 2013 evaluating patiromer in 306 outpatients with type 2 diabetes (estimated glomerular filtration rate, 15 to 5.0 mEq/L). All patients received RAAS inhibitors prior to and during study treatment. Patients were stratified by baseline serum potassium level into mild or moderate hyperkalemia groups and received 1 of 3 randomized starting doses of patiromer (4.2 g [n = 74], 8.4 g [n = 74], or 12.6 g [n = 74] twice daily [mild hyperkalemia] or 8.4 g [n = 26], 12.6 g [n = 28], or 16.8 g [n = 30] twice daily [moderate hyperkalemia]). Patiromer was titrated to achieve and maintain serum potassium level 5.0 mEq/L or lower. The primary efficacy end point was mean change in serum potassium level from baseline to week 4 or prior to initiation of dose titration. The primary safety end point was adverse events through 52 weeks. Secondary efficacy end points included mean change in serum potassium level through 52 weeks. A total of 306 patients were randomized. The least squares mean reduction from baseline in serum potassium level at week 4 or time of first dose titration in patients with mild hyperkalemia was 0.35 (95% CI, 0.22-0.48) mEq/L for the 4.2 g twice daily starting-dose group, 0.51 (95% CI, 0.38-0.64) mEq/L for the 8.4 g twice daily starting-dose group, and 0.55 (95% CI, 0.42-0.68) mEq/L for the 12.6 g twice daily starting-dose group. In those with moderate hyperkalemia, the

  15. Studies of Cystatin C joint eGFR-MDRD for diagnosis of early DN%胱抑素C联合eGFR-MDRD诊断早期DN的研究

    Institute of Scientific and Technical Information of China (English)

    吴丽婷; 刘梦琼

    2015-01-01

    Objective:Comparative analysis detection indexes such as [ inhibition C ( CysC ) , BUN, Scr, eGFR-MDRD and eGFR-cys] of application value in the diagnosis of early DN.Methods: Randomly collected the Type 2 diabetes ( Type 2 diabetes mellitus,T2DM)214 cases patients to our hospital check-up and 100 healthy people from January 2012 to October 2014,and according to the urinary albumin excretion rate could divided into pure T2DM group and diabetes group(DN group),collected the patient′s serum creatinine( Scr) ,urea nitrogen( BUN) ,serum[ inhibition( Cys C) detection indexes such as C,and USES the simplified MDRD formula according to the Scr estimate the eGFR(eGFR-MDRD),while using CysC CHINAcys equation calculated eGFR,eGFR-Cys],make statistical analysis for these measurements.Results: DN group compared with control group ,the BUN,Scr,CysC,eGFR-MDRD and eGFR-cys had significant changes,including BUN,Scr and CysC was significantly higher than control group;EGFR-MDRD and eGFR-cys significantly lower compared with control group,5 testing indexes,P<0.05.Five commonly used detection index curve of ROS area was greater than 0.5,and P<0.05.A single detection index of CysC indicators could reach 80.38,the highest followed by BUN and eGFR-MDRD;And joint detection of eGFR MDRD+Cys C much higher sensitivity and an index of eGFR-MDRD+eGFR-Cys and other individual detection index.Conclusion:eGFR-MDRD CysC indicator in the diagnosis of early DN screening is an effective,feasible, and the method of high accuracy.%目的:比较分析胱抑素C(CysC)、BUN、Scr、eGFR-MDRD 和eGFR-cys 等检测指标在诊断早期DN 中的应用价值。方法:随机收集2012年1月至2014年10月本院收治的2型糖尿病(Type 2 diabetes mellitus,T2DM)患者214例和来本院体检的100名健康人,并根据尿蛋白排泄率分为单纯的T2DM 组和糖尿病组(DN 组),收集患者的血肌酐(Scr)、尿素氮(BUN)、血清胱抑素C(Cys C)等检测

  16. Use of a fermented dairy probiotic drink containing Lactobacillus casei (DN-114 001) to decrease the rate of illness in kids: the DRINK study. A patient-oriented, double-blind, cluster-randomized, placebo-controlled, clinical trial.

    Science.gov (United States)

    Merenstein, D; Murphy, M; Fokar, A; Hernandez, R K; Park, H; Nsouli, H; Sanders, M E; Davis, B A; Niborski, V; Tondu, F; Shara, N M

    2010-07-01

    To evaluate whether a fermented dairy drink containing the probiotic strain Lactobacillus casei DN-114 001 could reduce the incidence of common infectious diseases (CIDs) and the change of behavior because of illness in children. We conducted a double-blinded, randomized, placebo-controlled allocation concealment clinical trial in the Washington, DC metropolitan area. Participants were 638 children 3-6 years old in daycare/schools. The intervention was a fermented dairy drink containing a specific probiotic strain or matching placebo with no live cultures for 90 consecutive days. Two primary outcomes were assessed: incidence of CIDs and change of behavior because of illness (both assessed by parental report). The rate of change of behavior because of illness was similar among active and control groups. However, the incidence rate for CIDs in the active group (0.0782) is 19% lower than that of the control group (0.0986) (incidence rate ratio=0.81, 95% CI: 0.65, 099) P=0.046. Daily intake of a fermented dairy drink containing the probiotic strain L. casei DN-114 001 showed some promise in reducing overall incidence of illness, but was primarily driven by gastrointestinal infections and there were no differences in change of behavior.

  17. 北疆水利枢纽灌溉引水倒虹工程DN2900缠绕玻璃钢夹砂管道监造皿坦

    Institute of Scientific and Technical Information of China (English)

    陈亮

    2015-01-01

    对北疆水利枢纽灌溉引水倒虹吸工程使用的DN2900缠绕玻璃钢夹砂管道生产进行监造,依据国标《玻璃纤维增强塑料夹砂管》及建设部给水排水设备产品质量监督检验中心玻璃纤维增强塑料夹砂管分中心所制定的《监造大纲》、《原材料生产工艺流程和管道检测的控制要求》、生产前及工艺过程的监控《监造实施细则》进行监督监造工作。主要工作总结如下。

  18. Observation of an extremely-long-lived metastable level in a Ti-like system via an L -shell dielectronic recombination measurement in highly charged 3 dn ions of tungsten

    Science.gov (United States)

    Tu, B.; Yao, K.; Shen, Y.; Yang, Y.; Li, M. C.; Xu, T. H.; Lu, Q. F.; Lu, D.; Wang, X.; Chen, C. Y.; Fu, Y.; Wei, B.; Zheng, C.; Huang, L. Y.; Xiong, G.; Yang, J. M.; Zhang, B. H.; Tang, Y. J.; Hutton, R.; Zou, Y.; Xiao, J.

    2017-09-01

    In this paper we report the L-shell dielectronic recombination measurement in highly charged 3 dn ions of tungsten by employing a fast electron beam-energy scanning technique at Shanghai EBIT. The studies of the LMM DR resonance strengths of Ar-like up to Mn-like tungsten were implemented through the experiment as well as a fully relativistic configuration interaction method in the flexible atomic code. In the analysis of DR spectrum, an isolated resonant peak was discovered coming from the DR processes via an extremely-long-lived metastable level in a Ti-like system. This work indicated the population of this Ti-like metastable level in the present EBIT plasma condition was as large as 45%.

  19. Validity and reliability of the Spanish version of the DN4 (Douleur Neuropathique 4 questions questionnaire for differential diagnosis of pain syndromes associated to a neuropathic or somatic component

    Directory of Open Access Journals (Sweden)

    Bouhassira Didier

    2007-12-01

    Full Text Available Abstract Background This study assesses the validity and reliability of the Spanish version of DN4 questionnaire as a tool for differential diagnosis of pain syndromes associated to a neuropathic (NP or somatic component (non-neuropathic pain, NNP. Methods A study was conducted consisting of two phases: cultural adaptation into the Spanish language by means of conceptual equivalence, including forward and backward translations in duplicate and cognitive debriefing, and testing of psychometric properties in patients with NP (peripheral, central and mixed and NNP. The analysis of psychometric properties included reliability (internal consistency, inter-rater agreement and test-retest reliability and validity (ROC curve analysis, agreement with the reference diagnosis and determination of sensitivity, specificity, and positive and negative predictive values in different subsamples according to type of NP. Results A sample of 164 subjects (99 women, 60.4%; age: 60.4 ± 16.0 years, 94 (57.3% with NP (36 with peripheral, 32 with central, and 26 with mixed pain and 70 with NNP was enrolled. The questionnaire was reliable [Cronbach's alpha coefficient: 0.71, inter-rater agreement coefficient: 0.80 (0.71–0.89, and test-retest intra-class correlation coefficient: 0.95 (0.92–0.97] and valid for a cut-off value ≥ 4 points, which was the best value to discriminate between NP and NNP subjects. Discussion This study, representing the first validation of the DN4 questionnaire into another language different than the original, not only supported its high discriminatory value for identification of neuropathic pain, but also provided supplemental psychometric validation (i.e. test-retest reliability, influence of educational level and pain intensity and showed its validity in mixed pain syndromes.

  20. Effects of consumption of a fermented dairy product containing the probiotic Lactobacillus casei DN-114 001 on common respiratory and gastrointestinal infections in shift workers in a randomized controlled trial.

    Science.gov (United States)

    Guillemard, Eric; Tanguy, Jérôme; Flavigny, Ann'Laure; de la Motte, Stephan; Schrezenmeir, Juergen

    2010-10-01

    The risk of infection may be increased in people under stress such as shift workers. This study examined the effect of a fermented dairy product containing the probiotic Lactobacillus casei DN-114 001 (verum) on the incidence of respiratory and gastrointestinal common infectious diseases (CIDs) and on immune functions in healthy shift workers. The study was single-center, randomized, double-blind, and controlled. Volunteers received 200 g/day of verum (n = 500) or control product (n = 500) for 3 months; 1-month follow-up was carried out. The cumulated number of CIDs (primary outcome) was not significantly different between groups. Because the Poisson distribution of the primary parameter did not fully fit the observed data, a post hoc categorical analysis was applied and showed a significantly lower cumulated number of CIDs in the verum group during the product consumption phase (odds ratio [OR] = 0.75, 95% confidence interval [CI] 0.59-0.95, p = 0.017). Verum also reduced the proportion of volunteers experiencing at least 1 CID (43% vs. 51%, p = 0.005), increased the time to the first occurrence of CID (p = 0.017) in the whole population, and reduced the cumulated number of CIDs in the subgroup of smokers (p = 0.033). In the course of CID, cumulated duration of fever was lower in the verum group (in the whole study phase) (p = 0.022), and an increase in leukocyte, neutrophil, and natural killer (NK) cell counts and activity (p = 0.047 to p DN-114 001 could reduce the risk of common infections in stressed individuals such as shift workers.

  1. Fermented milk containing Bifidobacterium lactis DN-173 010 improves gastrointestinal well-being and digestive symptoms in women reporting minor digestive symptoms: a randomised, double-blind, parallel, controlled study.

    Science.gov (United States)

    Guyonnet, Denis; Schlumberger, Armelle; Mhamdi, Leila; Jakob, Stefan; Chassany, Olivier

    2009-12-01

    The ability of probiotics to improve bowel habits or transit time has been shown in healthy populations. Additional data are required to support the use of specific probiotics to improve gastrointestinal (GI) well-being. The present study was designed to investigate the effect of consuming fermented milk (FM) on GI well-being, digestive symptoms and health-related quality of life (HRQoL) amongst women without diagnosed GI disorders. In this double-blind, controlled, parallel-design study, subjects were randomised to ingest daily either 2 x 125 g FM containing Bifidobacterium lactis DN-173 010 and yoghurt strains or a control non-fermented dairy product for 4 weeks followed by a 4-week wash-out period. GI well-being and digestive symptoms were assessed weekly. HRQoL was measured every 4 weeks. Data were analysed using analysis of covariance and logistic regression, correcting for baseline values on the full analysis set population of 197 women (aged 18-60 years). The percentage of women reporting an improvement in their GI well-being was significantly (P < 0.01) higher in the FM group v. the control group (OR 1.69; 95 % CI 1.17, 2.45). A significantly (P < 0.05) more pronounced decrease in the composite score of digestive symptoms was observed in the FM group when comparing with the control group (least squares mean - 0.57; 95 % CI - 1.12, - 0.02). Among HRQoL dimensions, the digestive comfort score was significantly (P < 0.05) improved in the FM group compared with the control group. The present study showed that the daily consumption of a specific FM is able to improve GI well-being and digestive symptoms in adult women without GI disorders.

  2. Pre- and post-natal melatonin administration partially regulates brain oxidative stress but does not improve cognitive or histological alterations in the Ts65Dn mouse model of Down syndrome.

    Science.gov (United States)

    Corrales, Andrea; Parisotto, Eduardo B; Vidal, Verónica; García-Cerro, Susana; Lantigua, Sara; Diego, Marian; Wilhem Filho, Danilo; Sanchez-Barceló, Emilio J; Martínez-Cué, Carmen; Rueda, Noemí

    2017-09-15

    Melatonin administered during adulthood induces beneficial effects on cognition and neuroprotection in the Ts65Dn (TS) mouse model of Down syndrome. Here, we investigated the effects of pre- and post-natal melatonin treatment on behavioral and cognitive abnormalities and on several neuromorphological alterations (hypocellularity, neurogenesis impairment and increased oxidative stress) that appear during the early developmental stages in TS mice. Pregnant TS females were orally treated with melatonin or vehicle from the time of conception until the weaning of the offspring, and the pups continued to receive the treatment from weaning until the age of 5 months. Melatonin administered during the pre- and post-natal periods did not improve the cognitive impairment of TS mice as measured by the Morris Water maze or fear conditioning tests. Histological alterations, such as decreased proliferation (Ki67+ cells) and hippocampal hypocellularity (DAPI+ cells), which are typical in TS mice, were not prevented by melatonin. However, melatonin partially regulated brain oxidative stress by modulating the activity of the primary antioxidant enzymes (superoxide dismutase in the cortex and catalase in the cortex and hippocampus) and slightly decreasing the levels of lipid peroxidation in the hippocampus of TS mice. These results show the inability of melatonin to prevent cognitive impairment in TS mice when it is administered at pre- and post-natal stages. Additionally, our findings suggest that to induce pro-cognitive effects in TS mice during the early stages of development, in addition to attenuating oxidative stress, therapies should aim to improve other altered processes, such as hippocampal neurogenesis and/or hypocellularity. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. QM/MM simulation (B3LYP) of the RNase A cleavage-transesterification reaction supports a triester A(N) + D(N) associative mechanism with an O2' H internal proton transfer.

    Science.gov (United States)

    Elsässer, Brigitta; Fels, Gregor; Weare, John H

    2014-01-22

    The mechanism of the backbone cleavage-transesterification step of the RNase A enzyme remains controversial even after 60 years of study. We report quantum mechanics/molecule mechanics (QM/MM) free energy calculations for two optimized reaction paths based on an analysis of all structural data and identified by a search for reaction coordinates using a reliable quantum chemistry method (B3LYP), equilibrated structural optimizations, and free energy estimations. Both paths are initiated by nucleophilic attack of the ribose O2' oxygen on the neighboring diester phosphate bond, and both reach the same product state (PS) (a O3'-O2' cyclic phosphate and a O5' hydroxyl terminated fragment). Path 1, resembles the widely accepted dianionic transition-state (TS) general acid (His119)/base (His12) classical mechanism. However, this path has a barrier (25 kcal/mol) higher than that of the rate-limiting hydrolysis step and a very loose TS. In Path 2, the proton initially coordinating the O2' migrates to the nonbridging O1P in the initial reaction path rather than directly to the general base resulting in a triester (substrate as base) AN + DN mechanism with a monoanionic weakly stable intermediate. The structures in the transition region are associative with low barriers (TS1 10, TS2 7.5 kcal/mol). The Path 2 mechanism is consistent with the many results from enzyme and buffer catalyzed and uncatalyzed analog reactions and leads to a PS consistent with the reactive state for the following hydrolysis step. The differences between the consistently estimated barriers in Path 1 and 2 lead to a 10(11) difference in rate strongly supporting the less accepted triester mechanism.

  4. QM/MM Simulation (B3LYP) of the RNase A Cleavage-Transesterification Reaction Supports a Triester AN+DN Associative Mechanism with an O2´ H Internal Proton Transfer

    Energy Technology Data Exchange (ETDEWEB)

    Elsasser, Brigitta M.; Fels, Gregor; Weare, John H.

    2014-01-22

    The mechanism of the backbone cleavage transesterification step of the RNase A enzyme remains controversial even after 60 years of study. We report QM/MM free energy calculations for two optimized reaction paths based on an analysis of all structural data and identified by a search for reaction coordinates using a reliable quantum chemistry method (B3LYP), equilibrated structural optimizations, and free energy estimations. Both paths are initiated by nucleophilic attack of the ribose O2? oxygen on the neighboring diester phosphate bond and both reach the same product state (PS) (a O3??O2? cyclic phosphate and a O5? hydroxyl terminated fragment). Path 1, resembles the widely accepted dianionic transition state (TS) general acid (His 119)/base (His 12) classical mechanism. However, this path has a barrier (25 kcal/mol) higher than that of the rate limiting hydrolysis step and a very loose TS. In Path 2, the proton initially coordinating the O2? migrates to the non-bridging O1P in the initial reaction path rather than directly to the general base resulting in a triester (substrate as base) AN+DN mechanism with a monoanionic weakly stable intermediate. The structures in the transition region are associative with low barriers (TS1 10, TS2 7.5 kcal/mol). The Path 2 mechanism is consistent with the many results from enzyme and buffer catalyzed and uncatalyzed analog reactions and leads to a PS consistent with the reactive state for the following hydrolysis step. The differences between the consistently estimated barriers in Path 1 and 2 lead to a 1011 difference in rate strongly supporting the less accepted triester mechanism.

  5. Leadership in non governmental sports organisations in Slovenia [Vůdcovská role v nevládních sportovních organizacích ve Slovinsku

    Directory of Open Access Journals (Sweden)

    Jakob Bednarik

    2010-12-01

    & Bednarik, 2006. Řízení tak významného zdroje je proto významným úkolem vedení slovinského sportu. CÍLE: Účelem této studie bylo odhalit stávající charakteristiky vůdcovské role dobrovolníků ve slovinských nevládních sportovních organizacích. METODIKA: Výzkum se prováděl na stratifikovaném vzorku 190 vedoucích nevládních sportovních organizací, kteří pracují v různých segmentech slovinského sportu. Měření různých stylů vedení bylo provedeno za pomoci dotazníku o způsobech vedení, zpracovaného na základě situačního modelu vedení Herseyho a Blancharda. VÝSLEDKY: Studie odhalila, že vedoucí sportovních organizací nejčastěji využívají koučování (selling – 42 % případů a podporování (participating – 37 %, zatímco přikazování (telling – 12 % a delegování (delegating – 9 % se používá mnohem méně. Vedoucí přizpůsobují styl vedení aktuálním okolnostem; ovšem nepoužívají vhodný styl vedení vzhledem k úrovni připravenosti svých svěřenců a okolností. Styl vedení nemá přímý dopad na měřené indikátory úspěšnosti sportovních organizací. Zjištění vedou k závěru, že vedoucí slovinských nevládních sportovních organizací obecně přehlížejí význam vůdcovství v tomto kontextu. ZÁVĚRY: Vzhledem k výše uvedeným průměrným výsledkům slovinského sportu lze vyvozovat, že dobrovolná práce ve sportovních organizacích představuje jednu z konkurenčních výhod v naší společnosti. Je proto nezbytné věnovat zvláštní pozornost jejímu rozvojovému potenciálu. Zjištění studie vedou k závěru, že by bylo vhodné ustavit národní strategii dobrovolné práce ve sportu.

  6. 活性维生素D对糖尿病肾病大鼠足细胞损伤的抑制作用及其机制研究%The effects of calcitriol on ameliorating podocytes impairment and its possible mechanism in DN rats

    Institute of Scientific and Technical Information of China (English)

    宋志霞; 郭银凤; 周敏; 张晓良

    2014-01-01

    Objective To investigate the effects and underlying mechanism of calcitriol on ameliorating podocytes impairment in DN rats.Methods SD rats were randomly divided into four groups:normal control (NC) group,calcitriol treatment (VD) group:calcitriol 0.1μg· kg--1 d-1,diabetic nephropathy (DN) group:streptozocin (STZ) 58 mg/kg,DN treated with calcitriol (DN + VD) group:calcitriol 0.1 μg · kg-1 · d-1 + STZ 58 mg/kg.Rats were sacrificed at the end of 18 weeks.Results Compared with the DN group,the DN + VD group exhibited significantly lower proteinuria by 36%,improved renal histology at the end of the experiment (P < 0.05),and similar levels of blood glucose,serum urea nitrogen as well as body weight (P > 0.05).There were no significant differences in the serum concentrations of creatinine,calcium and phosphorus among the four groups (P > 0.05).In DN group,the expressions of nephrin,podocin,VDR,PI3K-p85 and p-Akt were significantly decreased and the expression of desmin was increased compared to NC group.Calcitriol treatment could attenuate the above changes.Additionally,a positive correlation was observed between the expressions of nephrin and VDR (r=0.776,P < 0.05).Likewise,the expression of nephrin was positively correlated with either PI3K -p85 or p-Akt (r=-0.736,r=0.855,all P < 0.05).Conclusion Calcitriol can ameliorate podocytes injury in DN rats,which might be related with the further up-regulation of PI3K/p-Akt signaling pathway.%目的 探讨活性维生素D对糖尿病肾病大鼠足细胞损伤的抑制作用及其可能机制.方法 将SD雄性大鼠随机分为四组:对照组(NC组)、活性维生素D组(VD组,骨化三醇0.1 μg· kg-1·d-1灌胃)、糖尿病肾病组(DN组,腹腔注射STZ 58 mg/kg)、糖尿病肾病+活性维生素D组(DN+VD组).定期检测血糖、体质量,收集尿标本,18周末处死动物,检测Scr、BUN和尿蛋白变化.PAS及MASSON染色观察肾脏病理改变.电镜观察足细胞超

  7. Effect of the administration of a fermented milk containing Lactobacillus casei DN-114001 on intestinal microbiota and gut associated immune cells of nursing mice and after weaning until immune maturity

    Directory of Open Access Journals (Sweden)

    Carmuega Esteban

    2008-06-01

    Full Text Available Abstract Background Microbial colonization of the intestine after birth is an important step for the development of the gut immune system. The acquisition of passive immunity through breast-feeding may influence the pattern of bacterial colonization in the newborn. The aim of this work was to evaluate the effect of the administration of a probiotic fermented milk (PFM containing yogurt starter cultures and the probiotic bacteria strain Lactobacillus casei DN-114001 to mothers during nursing or their offspring, on the intestinal bacterial population and on parameters of the gut immune system. Results Fifteen mice of each group were sacrificed at ages 12, 21, 28 and 45 days. Large intestines were taken for determination of intestinal microbiota, and small intestines for the study of secretory-IgA (S-IgA in fluid and the study of IgA+ cells, macrophages, dendritic cells and goblet cells on tissue samples. The consumption of the PFM either by the mother during nursing or by the offspring after weaning modified the development of bifidobacteria population in the large intestine of the mice. These modifications were accompanied with a decrease of enterobacteria population. The administration of this PFM to the mothers improved their own immune system and this also affected their offspring. Offspring from mice that received PFM increased S-IgA in intestinal fluids, which mainly originated from their mother's immune system. A decrease in the number of macrophages, dendritic cells and IgA+ cells during the suckling period in offspring fed with PFM was observed; this could be related with the improvement of the immunity of the mothers, which passively protect their babies. At day 45, the mice reach maturity of their own immune system and the effects of the PFM was the stimulation of their mucosal immunity. Conclusion The present work shows the beneficial effect of the administration of a PFM not only to the mothers during the suckling period but also to

  8. Synthesis, characterization and thermal behaviour on solid pyruvates of light trivalent lanthanides; Sintese, caracterizacao e comportamento termico dos piruvatos de lantanideos leves no estado solido

    Energy Technology Data Exchange (ETDEWEB)

    Siqueira, A.B.; Carvalho, C.T. de; Ionashiro, M. [UNESP, Araraquara, SP (Brazil). Inst. de Quimica]. E-mail: massaoi@iq.unesp.br; Rodrigues, E.C. [Fundacao Educacional de Barretos, SP (Brazil); Ionashiro, E.Y.; Bannach, G. [Universidade Estadual de Ponta Grossa (UEPG), PR (Brazil). Dept. de Quimica

    2007-07-01

    Solid State Ln-L compounds, where Ln stands for light trivalent lanthanides (La - Gd) and L is pyruvate, have been synthesized. Thermogravimetry and derivative thermogravimetry (TG/DTG), differential scanning calorimetry (DSC), X-Ray powder diffractometry, infrared spectroscopy, elemental analysis, and complexometry were used to characterize and to study the thermal behaviour of these compounds. The results led to information about the composition, dehydration, ligand denticity, thermal behaviour and thermal decomposition of the isolated compounds. (author)

  9. Lymphoid EVA1 Expression Is Required for DN1-DN3 Thymocytes Transition

    NARCIS (Netherlands)

    Iacovelli, Stefano; Iosue, Ilaria; Di Cesare, Silvia; Guttinger, Maria

    2009-01-01

    Background: Thymus organogenesis and T lymphocyte development are accomplished together during fetal life. Proper development and maintenance of thymus architecture depend on signals generated by a sustained crosstalk between developing thymocytes and stromal elements. Any maturation impairment occu

  10. PROTECCION CONTRA EL ALCOHOLISMO POR EL POLIMORFISMO ARG47HIS DE LA DESHIDROGENASA ALCOHOLICA: DESARROLLO DE UN MODELO ANIMAL.

    OpenAIRE

    RIVERA MEZA, MARIO

    2009-01-01

    El alcoholismo es una de las adicciones de mayor prevalencia en el mundo. Sin embargo, se ha visto que la presencia de ciertos polimorfismos en los genes de las enzimas que metabolizan el etanol, son capaces de proteger a sus portadores de desarrollar est 114p.

  11. Asignación de nuevas funciones a la enzima gliceraldehído-3-fosfato deshidrogenasa en E. coli

    OpenAIRE

    Ferreira Melo, Elaine

    2012-01-01

    [spa] La identificación de interacciones proteína-proteína en las células resulta de gran importancia para entender los procesos biológicos a nivel molecular. Así, cada vez despierta mayor interés el estudio de las proteínas, no de manera individual sino a través de las interacciones que establecen con otras proteínas o biomoléculas ya que estas asociaciones son fundamentales para el desarrollo de las funciones celulares. En este sentido, una gran parte de este trabajo se ha dirigido a la apl...

  12. IKK2dn转染树突状细胞诱导产生的CD4+CD25-T细胞的筛选及鉴定%Screening and function identification of CD4 + CD25 - T cells induced by immature dendritic cells transfected with IKK2dn

    Institute of Scientific and Technical Information of China (English)

    樊彩斌; 张东兴; 温端改; 侯建全; 欧阳骏; 杜科霖

    2012-01-01

    Objective To investigate the method of screening CD4 + CD25 - T cells from regulatory T cells (Treg) induced by recipient-derived immature dendritic cells (imDC) transfected by IKK2dn and loaded with donor antigens,and assess their immunologic function.Methods Rat bone marrow-derived imDC were transfected by IKK2dn and loaded by BN antigen,then cultured with Lewis rats T cells in vitro.From these Treg,CD4 + CD25 T cells were screened by magnetic active cell sorting (MACS),then incubated in secondary mixed lymphocyte reaction ( MLR),with Lewis rat T lymphocytes.Results By MACS,the purity of CD4 + CD25 - T cells was more than 95%.The result of secondary MLR displayed the absorbance value was significantly lower in the CD4 + CD25 - T cells group ( 0.106 ± 0.006 ) than that in BN antigen group (0.189 ± 0.007 ),Adv0-CD4 +T cells group (0.419 ± 0.014) and the third donor group (0.200 ± 0.008) (P <0.05).Conclusion By MACS sereening,we can obtain high purity of CD4 + CD25-T cells from Treg induced by imDC transfected with IKK2dn and loaded by BN rat antigen and recipient Lewis rats T cells.And these T cells can inhibit T cell proliferation with the donor antigen specificity.%目的 探讨从转染IKK2dn并负载供者抗原的未成熟树突状细胞(imDC)诱导产生的调节性T细胞(Treg)中筛选CD4+ CD25 - Treg的方法,并进行鉴定.方法 Lewis大鼠骨髓源性imDC,转染IKK2dn后负载供者BN大鼠抗原,与Lewis大鼠T细胞进行体外混合淋巴细胞反应(MLR)诱导产生Treg,用免疫磁珠法(MACS)筛选出CD4+ CD25 -T细胞,流式细胞仪(FCM)检测细胞纯度.加入CD4+ CD25 -T细胞行再次MLR检测其抑制T细胞增殖的作用.结果 经MACS筛选,CD4+ CD25 -T细胞纯度为(95.78±1.25)%.再次MLR结果显示CD4+ CD25 -T细胞组的吸光度值为(0.106±0.006),低于BN抗原组(0.189±0.007)、Adv0-CD4+T细胞组(0.419±0.014)及第三方供者抗原组(0.200±0.008),差异有统计学意义(P<0.05).结论 转染IKK2dn并负载

  13. On realizations of exterior calculus with dN = 0

    Science.gov (United States)

    Abramov, V.

    1998-11-01

    We study realizations of the q-exterior calculus with exterior differential d satisfying d N = 0, N > 2 on the free associative algebra with one generator and on the generalized Clifford algebras. Analogs of the notions of connection and curvature are discussed in the case of the q-exterior calculus on the generalized Clifford algebra. We show that the q-exterior calculus on the free associative algebra with one generator is related to q-calculus on the braided line.

  14. Odpovědný management a odpovědné podnikání

    Directory of Open Access Journals (Sweden)

    Dytrt Zdenek

    2009-12-01

    Full Text Available Dynamika vývoje nových poznatků v technice, medicíně a v poslední době i farmacií vyvolávají potřebu inovovat i filozofii a obsah managementu a jeho úlohu v podnikatelském prostředí. Aktuálně na potřebu inovovat management upozorňuje současná celosvětová finanční a ekonomické krize. Její průběh vyžaduje analyzovat příčiny a nově definovat filo-zofii, obsah a cíle managementu, aby odpovídaly potřebám globalizace a 21. století. Sou-časná teorie managementu vznikla více než před sto léty a její modernizace se soustředila hlavně na automatizaci a zpracování dat kvantitativního vývoje podnikových a společenských jevů. Nové směry a cíle managementu by se měly více orientovat na odpovědnost, etické rozhodování a zvyšování pracovní motivace a kreativní plnění strategických cílů společnosti a podnikatelských aktivit.The dynamics of the development of new knowledge in technology, medicine and, more recently, pharmacy invokes the need to innovate the philosophy as well as management content and its role in the business environment. As it stands now, the need to innovate management is highlighted by the current global financial and economic crisis. Its course requires analyzing the causes and redefining the philosophy, content and objectives of management, to meet the needs of globalization and of the 21st century. The present theory of management was established more than a hundred years ago, and its modernization has focused mainly on the automation of processing the data of quantitative development of corporate and social phenomena. New directions and objectives of management should focus more on accountability, ethical decision-making and increasing the working motivation and creative fulfilment of strategic objectives and business activities.

  15. Malato deshidrogenasa citosólica de cisticercos de Taenia solium proveedora de malato : caracterización molecular, genómica y cinética /

    OpenAIRE

    Nava Balderas, Gabriela

    2011-01-01

     tesis que para obtener el grado de Doctor en Ciencias Biológicas, presenta Gabriela Nava Balderas ; asesor Agustín Plancarte Crespo, Juan Pedro Laclette San Román, Juan Luis Rendón Gómez. 106 páginas : ilustraciones, diagramas,. Doctorado en Ciencias Biológicas UNAM, Facultad de Medicina, 2011

  16. Discussion of changes of TGF-β1, Smad-4, α-SMA and PDGF in DN rat liver before and after treatment in traditional Chinese Medicine based on the "liver and kidney share the same origin"%基于“肝肾同源”探讨DN大鼠肝中TGF-β1、Smad-4、α-SMA和PDGF在中药治疗前后的变化

    Institute of Scientific and Technical Information of China (English)

    李光超; 车念聪; 耿建国; 赵晖; 王志强; 郭新新; 白辰

    2014-01-01

    目的:基于中医“肝肾同源”理论,观察中药方剂加味消渴康对糖尿病肾病(diabetic nephropahy,DN)大鼠肝组织转化生长因子-β1(transforming growth factor-β1,TGF-β1)、Smad-4蛋白(drosophila mothers against decapentaplegic protein-4)、α-平滑肌肌动蛋白(alpha smooth muscle actin,a-SMA)和血小板衍生生长因子(platelet-derived growth factor.PDGF)表达的影响.方法:采用单侧肾切除加链脲佐菌素(streptozotocin,STZ)诱导的糖尿病肾病大鼠模型,将造模成功后的DN大鼠按随机数字表法分为正常组、模型组、西药组和中药小、中、大剂量组.其中西药组给予氯沙坦灌胃,中药组给予加味消渴康灌胃,正常组及模型组每日灌服等量蒸馏水共12周.各组大鼠于第12周末处死取出肝脏,运用ELISA法观察大鼠肝组织TGFβ1、Smad-4、a-SMA和PDGF的表达.结果:与正常组比较,各组大鼠肝中TGFβ1、Smad-4、a-SMA和PDGF的表达明显升高,差异有统计学意义(P<0.01).中药治疗后,各组大鼠TGFβ1、Smad-4、a-SMA和PDGF的表达有一定程度的降低,差异有统计学意义(P<0.01或P<0.05).结论:加味消渴康对DN大鼠的肝损伤均有保护作用.

  17. Historic Archaeology of the Johnson (41DN248) and Jones (41DN250) Farmsteads in the Ray Roberts Lake Area: 1850-1950

    Science.gov (United States)

    1996-08-15

    92 7-5. Distribution of metal fragments in Block 1 ............................ 93 0 0 v *1 0 0 6 0S00 0 0 0 0 0 7-6. r:.-tograph of cast- iron ...elm, bois d’arc (Maclura pomifera) and mesquite ( Prosopis spp.) (Institute of Applied Sciences 1988:9-10; Yates and Ferring 1986:17). Along streams...accomplished using a carpenter’s tape. The height of the windmill had been changed by the addition of a new section of • angle iron , and numerous

  18. dnAGEnetics: genomic copy number variants and parental age as risk factors for psychiatric disorders

    NARCIS (Netherlands)

    Buizer - Voskamp, J.E.

    2011-01-01

    Introduction: Schizophrenia is a debilitating psychiatric disorder with a life-time risk of 0.46 – 1% in the general population. It is characterized by psychotic symptoms, including delusions and hallucinations. Its predisposition is influenced by a complex interaction of genetic and environmental f

  19. Disruption of Inhibitory Function in the Ts65Dn Mouse Hippocampus Through Overexpression of GIRK2

    Science.gov (United States)

    2007-10-24

    mouse cerebellum. Cerebellum 1:201- 206. Hattori M, Fujiyama A, Taylor TD, Watanabe H, Yada T, Park HS, Toyoda A, Ishii K, Totoki Y, Choi DK...mouse: A model of Down syndrome. J Comp Neurol 494: 815-833, 2006. Hattori M, Fujiyama A, Taylor TD, Watanabe H, Yada T, Park HS, Toyoda A, Ishii K...cultured from both DS patients and model animals are also reportedly more vulnerable to apoptosis ( Sawa , 1999). A majority of research investigating cell

  20. Revisiting Elastic Scattering of D(n, n)D reaction

    Science.gov (United States)

    Stanoiu, M.; Canton, L.; Kozier, K. S.; Nankov, N.; Plompen, A.; Rao, R.; Roubtsov, D.; Rouki, C.; Svenne, J. P.

    2010-04-01

    Interest has risen recently concerning the angular distribution of neutron elastic scattering on deuterium at low incident energies. The main subject is the amount of backscatter at energies below 3.2 MeV observed in differential cross-section measurements and represented in various evaluations. These various angles of approach encompass fundamental nuclear-data measurements, three-body nuclear-theory calculations, evaluated nuclear -data libraries and associated data processing, and the simulation of critical experiments involving heav y water. A new theoretical approach on the basis of three -nucleon theory was made that resulte d in new angular distributions. At the GELINA neutron time-of-flight facility a new experimental setup was developed to measure elastic scattering of neutrons on deuterium in the energy range of interest. The technique proposed is complementary to the earlier works by detecting the scattered neutron instead of the recoiling deuterium. The setup is an array of two HPGe detectors, each with a 10B neutron-gamma converter. Preliminary GELINA findings using a C6D6 target indicate less backscatter than predicted by ENDF/B -VII.0, in contrast to the nuclear-theory results. It is expected that completion of the planned work will reduce the uncertainty of the energy-angle distributions for deuterium elastic scattering and contribute to an improved deuterium evaluation in a future release of ENDF/B -VII.

  1. Zodpovědný cestovní ruch na Křivoklátsku

    OpenAIRE

    STAROSTOVÁ, Markéta

    2015-01-01

    This thesis deals with the responsible tourism in the selected area CHKO Křivoklátsko. The aim of the thesis is to evaluate the current state of eco-friendly types of tourismin this location through the analysis of its present supply. On the basis of the questionnaire research was also evaluated how are visitors interested in activities of responsible tourism. The data that were obtained through the analysis of supply and demand were used as a basis for suggestions of optimization of products...

  2. ScrumDN : Aplicación para gestión de proyectos con Scrum

    OpenAIRE

    Montesinos Marín, Oscar

    2014-01-01

    Memoria, presentación y código fuente del proyecto fin de carrera para crear una aplicación de gestión de proyectos que use la metodología Scrum. Memòria, presentació i codi font del projecte fi de carrera per crear una aplicació de gestió de projectes que utilitzi la metodologia Scrum.

  3. Promising New High-Explosives: Triaminoguanidinium (TAG) and Dinitramide (DN) Salts

    Science.gov (United States)

    2008-12-01

    thermal stability. Especially 4 shows a well- defined melting point in the range from 120 ° to 195 °C, which depends on the number of existing hydrogen...parameters (α, β, κ, θ) as stated below the equations and Xi being the mol fraction of i-th gaseous product, ki is the molar covolume of the i-th gaseous...ΔEUm° (J g -1) -5902 4888 −3699 -6168 -6360 6186 Explosion temp. (TE) (K) 3986 3210 2673 4710 4231 4657 Det. pressure (p) (kbar) 299 273 231

  4. Complexes of 3dn Metal Ions with Thiosemicarbazones: Synthesis and Antimicrobial Activity

    Directory of Open Access Journals (Sweden)

    Tudor Rosu

    2007-04-01

    Full Text Available The chelating behavior of the thiosemicarbazone derivatives of 2-hydroxy-8-R-tricyclo[7.3.1.0.2,7]tridecane-13-one (where R = H, CH3, C6H5 towards Co(II, Ni(II and Cu(II has been investigated by elemental analysis, molar conductivity measurements, UV-VIS, IR, ESR spectroscopy and thermal studies. It was deduced from the experiments performed that the ligands coordinate to metal ions in different ways – neutral bidentate or mononegative bidentate – depending on the nature of R. Also, if metal acetates are used instead of metal chlorides, the ligands coordinate in a mononegative bidentate fashion, regardless of the nature of R or the thiosemicarbazone type ligand. The antimicrobial activity of the ligands and of the complexes towards samples of Acinetobacter boumanii, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa was determined.

  5. Dynamics and bifurcations in a Dn-symmetric Hamiltonian network. Application to coupled gyroscopes

    Science.gov (United States)

    Buono, Pietro-Luciano; Chan, Bernard S.; Palacios, Antonio; In, Visarath

    2015-01-01

    The advent of novel engineered or smart materials, whose properties can be significantly altered in a controlled fashion by external stimuli, has stimulated the design and fabrication of smaller, faster, and more energy-efficient devices. As the need for even more powerful devices grows, networks have become popular alternatives to advance the fundamental limits of performance of individual units. In many cases, the collective rhythmic behavior of a network can be studied through the classical theory of nonlinear oscillators or through the more recent development of the coupled cell formalism. However, the current theory does not account yet for networks in which cells, or individual units, possess a Hamiltonian structure. One such example is a ring array of vibratory gyroscopes, where certain network topologies favor stable synchronized oscillations. Previous perturbation-based studies have shown that synchronized oscillations may, in principle, increase performance by reducing phase drift. The governing equations for larger array sizes are, however, not amenable to similar analysis. To circumvent this problem, the model equations are now reformulated in a Hamiltonian structure and the corresponding normal forms are derived. Through a normal form analysis, we investigate the effects of various coupling schemes and unravel the nature of the bifurcations that lead a ring of gyroscopes of any size into and out of synchronization. The Hamiltonian approach can, in principle, be readily extended to other symmetry-related systems.

  6. ScrumDN : Aplicación para gestión de proyectos con Scrum

    OpenAIRE

    Montesinos Marín, Oscar

    2014-01-01

    Memoria, presentación y código fuente del proyecto fin de carrera para crear una aplicación de gestión de proyectos que use la metodología Scrum. Memòria, presentació i codi font del projecte fi de carrera per crear una aplicació de gestió de projectes que utilitzi la metodologia Scrum.

  7. The Role of DN-GSK3b in Mammary Tumorigenesis

    Science.gov (United States)

    2007-07-01

    Publications Marganit Farago, Isabel Dominguez, Esther Landesman-Bollag, Xin Xu, Andrea Rosner, Robert D. Cardiff, and David C. Seldin . “Kinase Inactive...Isabel Dominguez, Esther Landesman , Xin Xu, and David C. Seldin Farago, June 2005. ”Kinase inactive GSK3b promotes Wnt signaling and mammary...Esther Landesman-Bollag, and David C. Seldin . 2004 “Kinase Inactive GSK3b Promotes Wnt Signaling and Mammary Tumorigenesis” BRCA (Boston Cancer

  8. InterProScan Result: DN237525 [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available 2.4e-56 T IPR011547 unintegrated Molecular Function: transporter activity (GO:0005215)|Biological Process: ...transport (GO:0006810)|Cellular Component: integral to membrane (GO:0016021)|Biological Process: transmembrane transport (GO:0055085) ...

  9. Infrared spectrum of the NH4-d(n)+ cation trapped in solid neon.

    Science.gov (United States)

    Jacox, Marilyn E; Thompson, Warren E

    2005-03-01

    The NH4+ cation has been stabilized in solid neon in sufficient concentration for the identification of both of its infrared-active vibrational fundamentals, which appear within a few wavenumbers of the gas-phase band centers. Systematic alteration of the concentrations and positions of introduction of NH3 and H2 in the discharge sampling experiments demonstrated that the highest yield of NH4+ resulted when both the NH3 and the H2 were introduced downstream from a discharge through pure neon. In this configuration, each of these molecules can be ionized by excited neon atoms and their resonance radiation (16.6 eV to 16.85 eV), but fragmentation is minimized. Both infrared-active vibrational fundamentals of ND4+ and several fundamentals of each of the partially deuterium-substituted isotopomers of NH4+ were also identified. Evidence is presented for complexation of NH4+ with an H atom or with one or more H2 molecules.

  10. $\\mathcal{N} = 1$ superconformal theories with $D_N$ blocks

    CERN Document Server

    Fazzi, Marco

    2016-01-01

    We study the chiral ring of four-dimensional superconformal field theories obtained by wrapping M5-branes on a complex curve inside a Calabi-Yau three-fold. We propose a field theoretic construction of all the theories found by Bah, Beem, Bobev and Wecht by introducing new building blocks, and prove several $\\mathcal{N} = 1$ dualities featuring the latter. We match the central charges with those computed from the M5-brane anomaly polynomial, perform the counting of relevant operators and analyze unitarity bound violations. As a byproduct, we compute the exact dimension of "heavy operators" obtained by wrapping an M2-brane on the complex curve.

  11. Coulomb branches for rank 2 gauge groups in 3dN=4 gauge theories

    Energy Technology Data Exchange (ETDEWEB)

    Hanany, Amihay [Theoretical Physics Group, Imperial College London,Prince Consort Road, London, SW7 2AZ (United Kingdom); Sperling, Marcus [Institut für Theoretische Physik, Leibniz Universität Hannover,Appelstraße 2, 30167 Hannover (Germany)

    2016-08-02

    The Coulomb branch of 3-dimensional N=4 gauge theories is the space of bare and dressed BPS monopole operators. We utilise the conformal dimension to define a fan which, upon intersection with the weight lattice of a GNO-dual group, gives rise to a collection of semi-groups. It turns out that the unique Hilbert bases of these semi-groups are a sufficient, finite set of monopole operators which generate the entire chiral ring. Moreover, the knowledge of the properties of the minimal generators is enough to compute the Hilbert series explicitly. The techniques of this paper allow an efficient evaluation of the Hilbert series for general rank gauge groups. As an application, we provide various examples for all rank two gauge groups to demonstrate the novel interpretation.

  12. Izrada 3D modela kućišta ravnog ventila DN 50

    OpenAIRE

    Hršak, Božidar; Golubić, Stjepan; Bošnjak, Matej

    2012-01-01

    Prikazana je izrada 3D modela kućišta centrifugalne pumpe na temelju postojećeg 2D nacrta gotovog proizvoda primjenom sinkrone tehnologije 3D modeliranja, i reverzibilnog inženjeringa. Na temelju kreiranog 3D modela, predložene su moguće modifikacije postojeće izvedbe proizvoda. Provedene su analize zakrivljenosti površina, Bézierove krivulje modela i 3D geometrije. Prikazana je i mogućnost vizualizacije gotovog 3D modela na Web stranicama u cilju prezentiranja proizvoda budućim kupcima primj...

  13. dnAGEnetics: genomic copy number variants and parental age as risk factors for psychiatric disorders

    NARCIS (Netherlands)

    Buizer - Voskamp, J.E.

    2011-01-01

    Introduction: Schizophrenia is a debilitating psychiatric disorder with a life-time risk of 0.46 – 1% in the general population. It is characterized by psychotic symptoms, including delusions and hallucinations. Its predisposition is influenced by a complex interaction of genetic and environmental

  14. Geometric engineering, mirror symmetry and 6{d}_{(1,0)}to 4{d}_{(N=2)}

    Science.gov (United States)

    Del Zotto, Michele; Vafa, Cumrun; Xie, Dan

    2015-11-01

    We study compactification of 6 dimensional (1,0) theories on T 2. We use geometric engineering of these theories via F-theory and employ mirror symmetry technology to solve for the effective 4d N=2 geometry for a large number of the (1 ,0) theories including those associated with conformal matter. Using this we show that for a given 6d theory we can obtain many inequivalent 4d N=2 SCFTs. Some of these respect the global symmetries of the 6d theory while others exhibit SL(2 , ℤ) duality symmetry inherited from global diffeomorphisms of the T 2. This construction also explains the 6d origin of moduli space of 4d affine ADE quiver theories as flat ADE connections on T 2. Among the resulting 4 d N=2 CFTs we find theories whose vacuum geometry is captured by an LG theory (as opposed to a curve or a local CY geometry). We obtain arbitrary genus curves of class S with punctures from toroidal compactification of (1 , 0) SCFTs where the curve of the class S theory emerges through mirror symmetry. We also show that toroidal compactification of the little string version of these theories can lead to class S theories with no punctures on arbitrary genus Riemann surface.

  15. Specifika klimatu na malotřídní škole

    OpenAIRE

    PRÁŠILOVÁ, Zdeňka

    2012-01-01

    The thesis will be devoted to issues of social and psychological climate in the classrooms and schools. It will be specifically aimed at describing the environment of at the schools with united classes.The practical part (research) will investigate the difference of socio-psychological climate in large schools and at the schools with united classes.

  16. Determinación de frecuencia cardiaca, frecuencia respiratoria, lactato deshidrogenasa, creatinkinasa y ácido láctico en caballos durante competencia de salto en la Sabana de Bogotá

    OpenAIRE

    Paula Andrea Guerrero Nieto

    2009-01-01

    El estudio presenta los primeros resultados de investigación en variables fisiológicas y sus cambios por el ejercicio en equinos en competencia de salto en el país. Se utilizaron 24 ejemplares de las razas Silla Argentina, PSI y mestizo de la Escuela de Equitación del Ejército Nacional de Colombia, que participaron en pruebas de salto (1,10 – 1,20 m) en diferentes centros ecuestres de la Sabana de Bogotá. Se determinó frecuencia cardiaca, frecuencia respiratoria y enzimas musculares (creatinq...

  17. Evaluación de actividad de fosfatasas y deshidrogenasas por efecto de la aplicación de vinazas en suelos cultivados con maíz dulce Zea mays L

    OpenAIRE

    Narvaez Castillo, Magda Cecilia

    2008-01-01

    La vinaza es un residuo resultante en el proceso de la producción de etanol, con potencialidades de fertilizante por su contenido de potasio. Sin embargo, presenta riesgos ambientales para las fuentes de agua por su alta demanda bioquímica de oxígeno. Las investigaciones sobre vinazas se han dirigido a los cambios fisicoquímicos que ocasiona en el suelo y escasamente a efectos en actividad biológica. Se realizó un experimento en el Invernadero de la Universidad Nacional de Colombia, Sede Palm...

  18. Hemólisis inducida por el ejercicio: relación entre el nivel de actividad de la glucosa-6-fosfato deshidrogenasa y el grado de hemólisis

    Directory of Open Access Journals (Sweden)

    Javier F. Bonilla

    2008-06-01

    Full Text Available Introducción: La oxidación, al igual que la hemólisis asociadas con el ejercicio tanto moderado como extenuante han sido condiciones objeto de múltiples estudios, pero sólo en algunos recientes se evaluó cómo las variaciones en la capacidad de los mecanismos antioxidantes enzimáticos eritrocitarios específicos y genéticamente determinados, condicionan distintos grados de susceptibilidad a la hemólisis inducida por el ejercicio, pero esos trabajos se han hecho sobre todo en individuos no sedentarios y con deficiencia franca de la enzima G6PD.Objetivo: El propósito de este trabajo fue determinar si existe o no relación entre el nivel de actividad de la enzima eritrocitaria G6PD y el grado de hemólisis inducida por el ejercicio en hombres adultos sedentarios en apariencia sanos, luego de un tiempo experimental de ejercicio moderado pre-extenuante y equivalente en modalidad y magnitud. Metodología: Se evaluaron 25 voluntarios hombres, sedentarios, en apariencia sanos; se les aplicó una sesión de ejercicio, sobre un cicloergómetro, de una hora de duración, a 70% del VO2pico previamente hallado. Sobre la base de la concentración de hemoglobina plasmática se calculó el porcentaje de hemólisis y la haptoglobina consumida, tomando en cuenta la variación en el plasma de la haptoglobina y la hemólisis intravascular inmediatamente después y hasta tres horas luego del ejercicio. Resultados: Se pudo determinar que los individuos presentaron, con base en la determinación de Hb plasmática libre, % de hemólisis y consumo de haptoglobina, hemólisis intravascular durante y hasta por lo menos tres horas post-ejercicio. Conclusiones: La magnitud de la hemólisis presentada, por un lado, tuvo una relación inversa significativa con la actividad de la G6PD, y por otro, que tal magnitud tuvo un comportamiento significativamente distinto por debajo del percentil 40 de la actividad hallada para G6PD y por encima de la misma, o sea, la respuesta hemolítica oxidativa en individuos sedentarios sanos con niveles más bajos de actividad de G6PD frente a una sesión de ejercicio a 70% del VO2pico presenta una gran variabilidad.

  19. Prevalencia de la deficiencia de glucosa - 6 - fosfato deshidrogenasa en donadores voluntarios de sangre que acuden a los hospitales nacionales Cayetano Heredia y Arzobispo Loayza: Lima - Perú

    Directory of Open Access Journals (Sweden)

    Wilson Ruiz

    1997-01-01

    Full Text Available Objetivos: El presente trabajo tuvo como objetivo, conocer la Prevalencia de la deficiencia de la G-6-PDH, en la población masculina mestiza, aparentemente sana que acude a los Bancos de Sangre de los hospitales nacionales Cayetano Heredia (HNCH y Arzobispo Loayza (HNAL. Material y métodos: Se examinó 140 muestras de hemodonadores entre junio y julio, 60 (42.85% correspondieron al HNCH y 80 (57.15% al HNCH. Se aplicó un cuestionario a todos los donantes indagando por: raza en los ancestros y antecedentes patológicos compatibles con episodios de hemólisis enzimopática. En todas las muestras se empleó el método cualitativo de Brewer (tamizaje y posteriormente fueron sometidos a la prueba cuantitativa SIGMA. Resultados: Con el método de Brewer resultaron 9 casos positivos (6.42% de los cuales sólo uno resultó positivo con el método confirmatorio, lo cual da una prevalencia de 0.71%. La totalidad de los participantes fueron de raza mestiza, carecían de antecedentes importantes, las edades estuvieron comprometidas entre los 18 y 58 años y el hematocrito entre 40 y 48%. Conclusiones: Se confirma que la prevalencia de esta deficiencia, en nuestra población es bastante baja. (Rev Med Hered 1997; 8: 11-18.

  20. DSD2D-FLS 2010: Bdzil's 2010 DSD Code Base; Computing tb and Dn with Edits to Reduce the Noise in the Dn Field Near HE Boundaries

    Energy Technology Data Exchange (ETDEWEB)

    Bdzil, John Bohdan [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2016-09-21

    The full level-set function code, DSD3D, is fully described in LA-14336 (2007) [1]. This ASCI-supported, DSD code project was the last such LANL DSD code project that I was involved with before my retirement in 2007. My part in the project was to design and build the core DSD3D solver, which was to include a robust DSD boundary condition treatment. A robust boundary condition treatment was required, since for an important local “customer,” the only description of the explosives’ boundary was through volume fraction data. Given this requirement, the accuracy issues I had encountered with our “fast-tube,” narrowband, DSD2D solver, and the difficulty we had building an efficient MPI-parallel version of the narrowband DSD2D, I decided DSD3D should be built as a full level-set function code, using a totally local DSD boundary condition algorithm for the level-­set function, phi, which did not rely on the gradient of the level-­set function being one, |grad(phi)| = 1. The narrowband DSD2D solver was built on the assumption that |grad(phi)| could be driven to one, and near the boundaries of the explosive this condition was not being satisfied. Since the narrowband is typically no more than10*dx wide, narrowband methods are discrete methods with a fixed, non-­resolvable error, where the error is related to the thickness of the band: the narrower the band the larger the errors. Such a solution represents a discrete approximation to the true solution and does not limit to the solution of the underlying PDEs under grid resolution.The full level-­set function code, DSD3D, is fully described in LA-14336 (2007) [1]. This ASCI-­supported, DSD code project was the last such LANL DSD code project that I was involved with before my retirement in 2007. My part in the project was to design and build the core DSD3D solver, which was to include a robust DSD boundary condition treatment. A robust boundary condition treatment was required, since for an important local “customer,” the only description of the explosives’ boundary was through volume fraction data. Given this requirement, the accuracy issues I had encountered with our “fast-­tube,” narrowband, DSD2D solver, and the difficulty we had building an efficient MPI-parallel version of the narrowband DSD2D, I decided DSD3D should be built as a full level-­set function code, using a totally local DSD boundary condition algorithm for the level-­set function, phi, which did not rely on the gradient of the level-­set function being one, |grad(phi)| = 1. The narrowband DSD2D solver was built on the assumption that |grad(phi)| could be driven to one, and near the boundaries of the explosive this condition was not being satisfied. Since the narrowband is typically no more than10*dx wide, narrowband methods are discrete methods with a fixed, non-resolvable error, where the error is related to the thickness of the band: the narrower the band the larger the errors. Such a solution represents a discrete approximation to the true solution and does not limit to the solution of the underlying PDEs under grid resolution.

  1. Multibeam collection for GRA03_01: Multibeam data collected aboard Granuaile from 2003-10-01 to 2003-10-12, departing from D?n Laoghaire, Ireland and returning to D?n Laoghaire, Ireland

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  2. Multibeam collection for KRY09_02: Multibeam data collected aboard Keary from 2009-07-23 to 2009-09-17, departing from D?n Laoghaire, Ireland and returning to D?n Laoghaire, Ireland

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  3. Multibeam collection for KRY10_01: Multibeam data collected aboard Keary from 2010-03-16 to 2010-04-16, departing from D?n Laoghaire, Ireland and returning to D?n Laoghaire, Ireland

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  4. Světové trendy v módním průmyslu

    OpenAIRE

    Hubková, Veronika

    2012-01-01

    The Dissertation is providing concise insight into the history of fashion industry, its global evolution and remarkable thresholds leading us into the way we can see it nowadays. Consequent focus is around today's trend of fast fashion being seen as the synonym for affordable and low-cost apparel. Vigorous supply chain analysis delivers valid arguments about negative social and environmental impact on our society as a result of recent fashion trends. Considering all above highlighted issues a...

  5. Průmysl 4.0 a společenskovědní výzkum

    Directory of Open Access Journals (Sweden)

    Jiří Vacek

    2017-06-01

    Full Text Available The contribution is based on the desk research of exponentially growing number of publications devoted socio-economic issues related to Industry 4.0. The growing interest in Industry 4.0 is documented by growing interest in documents with this keyword in Google searches. The brief introduction refers to importance of research in social sciences for successful implementation of this concept. This introduction is followed by section 1 with 4 subsections attempting to answer questions related to socio-economic aspects of Industry 4.0: management in the age of machines, competencies and skills for work with clever machines, analysis of jobs left for people and preparation for the future. In the conclusion are expressed some gaps in the Czech Republic R&D and educational systems related to Industry 4.0.

  6. DNC/HNC and N2D+/N2H+ ratios in high-mass star forming cores

    CERN Document Server

    Fontani, F; Furuya, K; Sakai, N; Aikawa, Y; Yamamoto, S

    2014-01-01

    Chemical models predict that the deuterated fraction (the column density ratio between a molecule containing D and its counterpart containing H) of N2H+, Dfrac(N2H+), is high in massive pre-protostellar cores and rapidly drops of an order of magnitude after the protostar birth, while that of HNC, Dfrac(HNC), remains constant for much longer. We tested these predictions by deriving Dfrac(HNC) in 22 high-mass star forming cores divided in three different evolutionary stages, from high-mass starless core candidates (HMSCs, 8) to high-mass protostellar objects (HMPOs, 7) to Ultracompact HII regions (UCHIIs, 7). For all of them, Dfrac (N2H+) was already determined through IRAM-30m Telescope observations, which confirmed the theoretical rapid decrease of Dfrac(N2H+) after protostar birth (Fontani et al. 2011). Therefore our comparative study is not affected by biases introduced by the source selection. We have found average Dfrac(HNC) of 0.012, 0.009 and 0.008 in HMSCs, HMPOs and UCHIIs, respectively, with no stati...

  7. DNC/HNC and N2D+/N2H+ ratios in high-mass star-forming cores

    Science.gov (United States)

    Fontani, F.; Sakai, T.; Furuya, K.; Sakai, N.; Aikawa, Y.; Yamamoto, S.

    2014-05-01

    Chemical models predict that the deuterated fraction (the column density ratio between a molecule containing D and its counterpart containing H) of N2H+, Dfrac(N2H+), high in massive pre-protostellar cores, is expected to rapidly drop by an order of magnitude after the protostar birth, while that of HNC, Dfrac(HNC), remains constant for much longer. We tested these predictions by deriving Dfrac(HNC) in 22 high-mass star-forming cores divided in three different evolutionary stages, from high-mass starless core candidates (HMSCs, eight) to high-mass protostellar objects (HMPOs, seven) to ultracompact H II regions (UCHIIs, seven). For all of them, Dfrac(N2H+) was already determined through IRAM 30 m Telescope observations, which confirmed the theoretical rapid decrease of Dfrac(N2H+) after protostar birth. Therefore, our comparative study is not affected by biases introduced by the source selection. We have found average Dfrac(HNC) of 0.012, 0.009 and 0.008 in HMSCs, HMPOs and UCHIIs, respectively, with no statistically significant differences among the three evolutionary groups. These findings confirm the predictions of the chemical models, and indicate that large values of Dfrac(N2H+) are more suitable than large values of Dfrac(HNC) to identify cores on the verge of forming high-mass stars, likewise what was found in the low-mass regime.

  8. Diverzita půdních mikroorganismů ve vztahu k biodegradaci polymerů

    OpenAIRE

    Hušek, Pavel

    2016-01-01

    Polymer materials have had a big impact on the industry in the 20th century and replaced a large amount of then used materials. Plastic waste, however, has subsequently become a big problem that needs a solution. One of the possible solutions is to produce biodegradable plastics, that should degrade in nature by themselves and therefore not increase the volume of plastic waste. Their degradation, however, is often only partial and the dynamics of polymer biodegradation in soil are not yet ful...

  9. Biological properties of Staphylococcus genus strains from patients of Mechnikov Dnіpropetrovsk region clinical hospital

    Directory of Open Access Journals (Sweden)

    Y. Y. Nikulina

    2011-04-01

    Full Text Available It was investigated the biological properties of 494 strains of Staphylococcus genus isolated from pathological material of patients from 15 Departments of Mechnikov Dnipropetrovsk Region Clinical Hospita. All microflora was presented by four bacteria species: Staphylococcus aureus, S. epidermidis, S. haemolyticus and S. saprophyticus. S. aureus was the most extended agent of infections. All bacteria were the most sensitive to linezolid, gentamycin and meropenem.

  10. Ionization energies of three resonance-stabilized radicals: cyclohexadienyl (dn, n = 0, 1, 6, 7), 1-phenylpropargyl, and methylcyclohexadienyl.

    Science.gov (United States)

    Krechkivska, Olha; Wilcox, Callan; O'Connor, Gerard D; Nauta, Klaas; Kable, Scott H; Schmidt, Timothy W

    2014-11-06

    The ionization energies for three resonance-stabilized radicals are determined: cyclohexadienyl, 1-phenylpropargyl, and methylcyclohexadienyl. The recommended ionization energies are, respectively, 6.820(1), 6.585(1), and 7.232(1) eV. That of cyclohexadienyl is found to be just 0.02 eV above a high level ab initio calculation [Bargholz, A.; Oswald, R.; Botschwina, P. J. Chem. Phys. 2013, 138, 014307], and that of 1-phenylpropargyl is found within the stated error of a recent experimental determination [Holzmeier, F.; Lang, M.; Hemberger, P.; Fischer, I. ChemPhysChem 2014, DOI: 10.1002/cphc.201402446]. The ionization energy of the methylcyclohexadienyl radical is consistent with the ortho isomer. Ionization energies of a range of isotopologues of cyclohexadienyl radical are given, along with their D1 ← D0 origin band positions, which indicate a blue shift of 18 cm(-1) per deuterium atom substituted. The ionization energy of cyclohexadienyl, along with the calculated bond dissociation energy of Bargholz et al., affords a new estimate of the 0 K proton affinity of benzene: 739.7 ± 2.0 kJ/mol. The ionization energies are discussed in terms of the interplay between radical and cation stabilization energies.

  11. The role of intrahepatic CD3+/CD4-/CD8- double negative T (DN T) cells in enhanced acetaminophen toxicity.

    Science.gov (United States)

    Getachew, Yonas; Cusimano, Frank A; James, Laura P; Thiele, Dwain L

    2014-10-15

    The role of the immune system, specifically NK, NKT and CD3 cells, in acetaminophen (APAP) induced liver injury remains inconsistently defined. In the present study, wild type (C57BL/6J) mice and granzyme B deficient (GrB -/-) mice were treated with acetaminophen to assess the role of the immune system in acute liver injury. Doses of acetaminophen that induced sub lethal liver injury in wild type mice unexpectedly produced fatal hepatotoxicity in granzyme B deficient (GrB -/-) mice. Analysis revealed that GrB -/- mice had an increased population of intrahepatic CD3 (+), CD4 (-), and CD8 (-) lymphocytes expressing the CD69 activation marker and Fas ligand. Depletion of these cells in the GrB -/- and wild type mice made them less susceptible to APAP injury, while depletion of NK1.1 (+) cells or both CD4 (+) and CD8 (+) T cells failed to provide the same hepatoprotection. Transfer of the GrB -/- IHLs further exacerbated liver injury and increased mortality in wild type mice but not in LRP/LPR mice, lacking fas expression. Acetaminophen toxicity is enhanced by the presence of activated, FasL expressing intrahepatic CD3 (+), CD4 (-), CD8 (-), NK1.1 (-) T cells. Depletion of these cells from GrB -/- mice and wild type mice greatly reduces mortality and improves the course of liver injury recovery. Published by Elsevier Inc.

  12. A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance.

    Science.gov (United States)

    Gabor, K A; Charette, J R; Pietraszewski, M J; Wingfield, D J; Shim, J S; Millard, P J; Kim, C H

    2015-08-01

    Melanoma Differentiation-Associated protein 5 (MDA5) is a member of the retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family, which is a cytosolic pattern recognition receptor that detects viral nucleic acids. Here we show an Mda5-dependent response to rhabdovirus infection in vivo using a dominant-negative mda5 transgenic zebrafish. Dominant-negative mda5 zebrafish embryos displayed an impaired antiviral immune response compared to wild-type counterparts that can be rescued by recombinant full-length Mda5. To our knowledge, we have generated the first dominant-negative mda5 transgenic zebrafish and demonstrated a critical role for Mda5 in the antiviral response to rhabdovirus. Copyright © 2015 Elsevier Ltd. All rights reserved.

  13. Estudio de pacientes con aciduria glutarica tipo II, mediante la incubacion de fibroblastos con acidos palmitico y miristico tritiados

    National Research Council Canada - National Science Library

    Osorio Orozco, Jose Henry

    2011-01-01

    Introduccion: la aciduria glutarica tipo II, o deficiencia multiple de acil-CoA deshidrogenasas, es un trastorno causado por deficiencia de la flavoproteina de transferencia de electrones, de su oxidorreductasa o de ambas...

  14. Identificação de Mycobacterium bovis em cepas micobacterianas isoladas espécimes clínicos humanos em um complexo hospitalar na cidade do Rio de Janeiro

    OpenAIRE

    Sobral,Luciana Fonseca; Duarte, Rafael Silva; Vieira,Gisele Betzler de Oliveira; Silva,Marlei Gomes da; Boechat, Neio; Fonseca,Leila Souza

    2011-01-01

    Entre 2005 e 2006, 8.121 espécimes clínicos enviados ao Laboratório de Micobactérias do Hospital Universitário Clementino Fraga Filho/Instituto de Doenças do Tórax, no Rio de Janeiro, RJ, foram inoculados em meio Löwenstein-Jensen contendo glicerol e piruvato. Desses espécimes, 79 isolados de micobactérias tiveram crescimento somente em meio com piruvato, sendo selecionados para a identificação presuntiva de Mycobacterium bovis. Esses isolados foram submetidos à identificação por testes bioqu...

  15. The role of intrahepatic CD3 +/CD4 −/CD8 − double negative T (DN T) cells in enhanced acetaminophen toxicity

    Energy Technology Data Exchange (ETDEWEB)

    Getachew, Yonas, E-mail: yonas.getachew@utsouthwestern.edu [Division of Digestive and Liver Diseases, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9151 (United States); Cusimano, Frank A. [Division of Digestive and Liver Diseases, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9151 (United States); James, Laura P. [Department of Pediatrics, University of Arkansas, Little Rock, AR (United States); Thiele, Dwain L. [Division of Digestive and Liver Diseases, Department of Internal Medicine, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9151 (United States)

    2014-10-15

    The role of the immune system, specifically NK, NKT and CD3 cells, in acetaminophen (APAP) induced liver injury remains inconsistently defined. In the present study, wild type (C57BL/6J) mice and granzyme B deficient (GrB −/−) mice were treated with acetaminophen to assess the role of the immune system in acute liver injury. Doses of acetaminophen that induced sub lethal liver injury in wild type mice unexpectedly produced fatal hepatotoxicity in granzyme B deficient (GrB −/−) mice. Analysis revealed that GrB −/− mice had an increased population of intrahepatic CD3 (+), CD4 (−), and CD8 (−) lymphocytes expressing the CD69 activation marker and Fas ligand. Depletion of these cells in the GrB −/− and wild type mice made them less susceptible to APAP injury, while depletion of NK1.1 (+) cells or both CD4 (+) and CD8 (+) T cells failed to provide the same hepatoprotection. Transfer of the GrB −/− IHLs further exacerbated liver injury and increased mortality in wild type mice but not in LRP/LPR mice, lacking fas expression. Conclusions: Acetaminophen toxicity is enhanced by the presence of activated, FasL expressing intrahepatic CD3 (+), CD4 (−), CD8 (−), NK1.1 (−) T cells. Depletion of these cells from GrB −/− mice and wild type mice greatly reduces mortality and improves the course of liver injury recovery. - Highlights: • Intrahepatic lymphocytes (IHLs) from GrB −/− mice harbor activated DNT cells. • IHLs from GrB −/− mice exhibit enhanced Fas ligand expression. • Acetaminophen toxicity is enhanced by activated, FasL expressing DNT cells.

  16. Quark model description of the \\Lambda_c(2940)^+ as a molecular D^*N state and the possible existence of the \\Lambda_b(6248)

    CERN Document Server

    Ortega, P G; Fernández, F

    2012-01-01

    The \\Lambda_c(2940)^+ baryon is studied in a constituent quark model as a molecular state composed by nucleons and D^* mesons. A bound state with the right binding energy is found for the J^\\pi=3/2^- channel. The partial widths \\Lambda_c(2940)^+\\to ND and \\Lambda_c(2940)^+\\to \\Sigma_c \\pi are calculated and the results are consistent with the experimental data. Additionally a bottom partner \\bar B^*N is predicted with a mass of 6248 MeV/c^2.

  17. Důsledky vlivu CSR na výkonnost podnikatelských subjektů a nevládní neziskové organizace

    OpenAIRE

    Březinová, Anna

    2014-01-01

    This thesis deals with the issue of corporate social responsibility. It examined the impact of CSR on performance of companies with a focus on financial and non-financial effects of this concept. The key survey was carried out in enterprises and non-profit organizations in Brno. Publicly available annual reports were used to analyze changes in financial indicators before and after launching concept CSR within organization. The benefit of this work is to determine the status of CSR among respo...

  18. Vliv institucionálního zapojení nevládních organizací na volbu strategií

    Directory of Open Access Journals (Sweden)

    Aneta Pinková

    2013-04-01

    Full Text Available The article explores institutional platforms used to incorporate non-governmental groups into the decision making process and the possible impacts of such incorporation on their choice of strategy. The paper starts with an overview of relevant theories and concepts, such as the political opportunities structures, the concept of access points to the political system and, most importantly, the insider – outsider typology of interest groups. The empirical part of the paper focuses on the Czech Republic and the two institutional platforms available to NGOs within its political system: participation in advisory committees and formal commenting procedure. The article further presents a case study of employer and business groups in the Czech Republic, using a scale of institutional incorporation as an independent variable influencing the groups’ strategy choice. The scale roughly indicates the level of the organizations’ institutional incorporation, based on their involvement in both advisory committees and formal commenting procedure. On the basis of the findings from the case study, possible impacts of institutional incorporation on strategy choice are outlined, indicating that the so-called insider strategies are more often used by more incorporated groups. This suggests a continuing relevance of Grant’s insider – outsider typology, which has been questioned by political scientists in recent years. While the article focuses on a relatively narrowly defined category of organizations, the findings also suggest that the role and influence of institutionalized platforms on the overall strategy choice of traditional interest groups should not be ignored by researchers, as it is often the case both in the Czech Republic and internationally.

  19. Study Protocol Effect of the consumption of a fermented dairy product containing Bifidobacterium lactis DN-173 010 on constipation in childhood: a multicentre randomised controlled trial (NTRTC: 1571)

    NARCIS (Netherlands)

    Tabbers, M.M.; Chmielewska, A.; Roseboom, M.G.; Boudet, C.; Perrin, C.; Szajewska, H.; Benninga, M.A.

    2009-01-01

    ABSTRACT: BACKGROUND: Constipation is a frustrating symptom affecting 3% of children worldwide. Randomised controlled trials show that both polyethylene glycol and lactulose are effective in increasing defecation frequency in children with constipation. However, in 30-50%, these children reported ab

  20. FIFTH SEMINAR IN MEMORY OF D.N. KLYSHKO: Dynamics of a Fabry-Perot cavity in the field of a plane gravitational wave

    Science.gov (United States)

    Tarabrin, S. P.

    2007-12-01

    The interaction of a weak gravitational wave with a Fabry-Perot cavity is analysed beyond the long-wavelength approximation in the input-mirror locally Lorentzian frame of reference taking the light pressure into account. The generalised expressions are obtained for the coefficient of pondermotive optical rigidity, the motion law of the moving mirror of the cavity and the response function of the cavity. It is shown that the latter is a sum of two phase shifts of a circulating light wave: the phase incursion after reflection from the moving mirror and the phase incursion due to the direct interaction of gravitational and light waves in the cavity. The possibility of the resonance detection of high-frequency gravitational waves by using the optical rigidity effect is considered.

  1. Determining the resonance strength of the 56Ni rp-process waiting point through (d,n) with VANDLE and MoNA-LISA

    Science.gov (United States)

    Peters, W.; Grzywacz, R.; Madurga, M.; Paulauskas, S. V.; Taylor, S.; Allen, J.; Cizewski, J. A.; Manning, B.; Howard, M. E.; Smith, J.; Jones, M.; Baumann, T.; Thoennessen, M.; Bardayan, D. W.; Pain, S. D.; Clement, R. C. C.; Brown, J.; Luther, B.; Ilyushkin, S.; O'Malley, P. D.; Ikeyama, R.; Kozub, R. L.; Bergstrom, Z. J.; Deyoung, P. A.; Rogers, W.

    2014-03-01

    The rapid proton capture (rp) process of explosive nucleosynthesis is believed to be the driver of X-ray bursts and creates nuclei up to around mass 110. Whereas much of this process burns in an equilibrium determined by half-lives and masses, the waiting point at 56Ni is unique. At this point the process reaches its peak luminosity and the synthesis of almost all heavier nuclei pass through the 56Ni(p,γ)57Cu reaction. Since the gamma-decay width dominates the relevant resonance in 57Cu, a measurement of its proton partial width can be used to extract the proton-capture resonance strength. An experiment to do this was performed at the NSCL using the Versatile Array of Neutron Detectors at Low Energy (VANDLE) along with the MoNA-LISA neutron detector arrays; and was the commissioning experiment for VANDLE with a transfer reaction. The events in the digitizing electronics of VANDLE were event-matched to the MoNA-LISA-Sweeper data acquisition system. Work supported in part by U.S. DOE, the NNSA SSAA, and the NSF.

  2. Amyloidogenic Properties of a D/N Mutated 12 Amino Acid Fragment of the C-Terminal Domain of the Cholesteryl-Ester Transfer Protein (CETP

    Directory of Open Access Journals (Sweden)

    Victor García-González

    2011-03-01

    Full Text Available The cholesteryl-ester transfer protein (CETP facilitates the transfer of cholesterol esters and triglycerides between lipoproteins in plasma where the critical site for its function is situated in the C-terminal domain. Our group has previously shown that this domain presents conformational changes in a non-lipid environment when the mutation D470N is introduced. Using a series of peptides derived from this C-terminal domain, the present study shows that these changes favor the induction of a secondary β-structure as characterized by spectroscopic analysis and fluorescence techniques. From this type of secondary structure, the formation of peptide aggregates and fibrillar structures with amyloid characteristics induced cytotoxicity in microglial cells in culture. These supramolecular structures promote cell cytotoxicity through the formation of reactive oxygen species (ROS and change the balance of a series of proteins that control the process of endocytosis, similar to that observed when β-amyloid fibrils are employed. Therefore, a fine balance between the highly dynamic secondary structure of the C-terminal domain of CETP, the net charge, and the physicochemical characteristics of the surrounding microenvironment define the type of secondary structure acquired. Changes in this balance might favor misfolding in this region, which would alter the lipid transfer capacity conducted by CETP, favoring its propensity to substitute its physiological function.

  3. A DN-mda5 Transgenic Zebrafish 1 Model Demonstrates that Mda5 Plays an Important Role in Snakehead Rhabdovirus Resistance

    Science.gov (United States)

    Gabor, KA; Charette, JR; Pietraszewski, MJ; Wingfield, DJ; Shim, JS; Millard, PJ; Kim, CH

    2015-01-01

    Melanoma Differentiation-Associated protein 5 (MDA5) is a member of the retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family, which is a cytosolic pattern recognition receptor that detects viral nucleic acids. Here we show an Mda5-dependent response to rhabdovirus infection in vivo using a dominant-negative mda5 transgenic zebrafish. Dominant-negative mda5 zebrafish embryos displayed an impaired antiviral immune response compared to wild-type counterparts that can be rescued by recombinant full-length Mda5. To our knowledge, we have generated the first dominant-negative mda5 transgenic zebrafish and demonstrated a critical role for Mda5 in the antiviral response to rhabdovirus. PMID:25634485

  4. Quantum and quasi-classical dynamics of reaction H + DN (v = 0,1; j = 0) → HD + N and its isotopic variants

    Science.gov (United States)

    Li, Dan; Wang, Yuliang; Wumaier, Tuerdi

    2016-08-01

    Time-dependent wave packet (TDWP) and quasic-classical trajectory (QCT) methods were employed to study the title reaction and its isotopic variants on a new potential energy surface (PES) of 4A'' state over the range of collision energies, starting from the reaction threshold to 1.0 eV. We took the Coriolis coupling (CC) effect into account and presented the comparison between the CC and the centrifugal sudden (CS) approximation calculations. Reaction probabilities obtained using the different methods were thoroughly compared as a function of the collision energy for a series of total angular momentum J values. Significant differences of integral cross section between CC and CS results have been observed. The QCT-calculated cross sections and probabilities reproduced well the CC results over most of the collision energy range. In addition, the attack angle dependent reaction probabilities of the title reaction at J = 0 were calculated. The reaction probabilities were found to be strongly dependent on the attack angle. The influences of the reactant vibration excitation on the attack angle dependent reaction probabilities were also studied in detail.

  5. LFP: A PC-program for ligand-field analysis of 3d(n) ions in Oh and lower symmetries

    Science.gov (United States)

    Kurzak

    2000-05-01

    A modular and efficient version of PC program for calculating ligand-field parameters, i.e. crystal-field model (CFM) and angular overlap model (AOM) is presented. The LFP program is designed to calculate the ligand-field parameters of low symmetry transition metal complexes. It is based on the general method for the analysis of central ion states distortion using group theory. The program has not closed form. It will be extended, according to spectroscopic studies in our laboratory. It is written in FORTRAN language.

  6. Performance of 40-millimeter-bore ball bearings with lead- and lead-alloy-plated retainers in liquid hydrogen at 1.2 million DN

    Science.gov (United States)

    Brewe, D. E.; Wisander, D. W.; Scribbe, H. W.

    1972-01-01

    Forty-millimeter-bore ball bearings with lead- and lead-alloy-coated retainers were operated in liquid hydrogen at 30,000 rpm under a thrust load of 1780 N (400 lb.) Four different substrate materials were used for the retainer. Longer bearing run times were achieved with a lead-tin-copper alloy coating plated onto a leaded-bronze material (22.5 hr) and an aluminum-bronze alloy (19.3 hr). One bearing with a pure lead coating achieved the desired objective of 10 hr. This bearing had an aluminum - bronze substrate retainer and ran successfully for 12.4 hr. Additions of antimony to the lead provided an alloy coating with better wear resistance than pure lead; however, this coating was abrasive to the outer-race lands.

  7. Performance of high speed ball bearings with lead and lead alloy plated retainers in liquid hydrogen at 1.2 million DN

    Science.gov (United States)

    Brewe, D. E.; Scibbe, H. W.; Wisander, D. W.

    1973-01-01

    Ball bearings with lead- and lead-alloy-coated retainers were operated in liquid hydrogen at 30,000 rpm under a thrust load of 400 lb. Bearing lives were compared using different: (1) lead- and lead-alloy coatings, (2) coating thicknesses, (3) substrate materials, (4) retainer locating surfaces, and (5) plating techniques. Longer bearing run times were achieved using retainers with a lead-tin-copper alloy coating electroplated onto a leaded-bronze material and an aluminum-bronze alloy. Thirty percent of the bearings tested achieved the desired objective of 10 hours. All of the lead-alloy coated retainers exceeded this objective. A coating thickness of at least 0.0014 in. was used for all bearings exceeding the 10-hour goal.

  8. Španělský módní průmysl a jeho místo ve světě

    OpenAIRE

    OLIVOVÁ, Klára

    2012-01-01

    Klára Olivová Spanish fashion industry and its place in the world The topic of this thesis is Spanish fashion industry from the 19.century until now. This thesis brings the entire analyses of the contemporary fashion industry and information about the main representatives of Spanish fashion: their business strategies and their position on the European and world market. At the end I will also mention the penetration of the Spanish fashion companies on the Czech market and I will try to explain...

  9. 结构DN A纳米新技术的研究现状与应用%Research status and application of structural DNA nanotechnology

    Institute of Scientific and Technical Information of China (English)

    黄春; 侯贺伟; 叶盟盟; 李萌萌; 崔光照; 王延峰

    2014-01-01

    Structural DNA nanotechnology was systematic summarized,including the research status of DNA self-assembly,DNA origami and SST self-assembly.In the design of Zhengzhou University of Light Industry badge for instance,DNA origami and SST self-assembly were introduced to devise and establish DNA struc-tural model of school badge with hollow structure.With this example,structural DNA nanotechnology was valued and investigated.The main application of the technique was in the aspects of the assembly of guiding inorganic nanoparticles with precision,the equipment of nano-bio-chip,as well as the combination with micro-machining technology.In view of this it was proposed that structural DNA nanotechnology would play a more active role in the application field of nano-electronic circuits and devices,nano-optoelectronics,bio-sensors of high sensitivity and high specificity,molecular robotics,materials science and nano-medicine etc.in future development.%综述了结构DNA纳米技术---DNA自组装、DNA折纸术和SST自组装的研究现状,并以郑州轻工业学院校徽设计中引入DNA折纸术构建校徽DNA结构模型、利用SST自组装方法设计构造镂空结构的校徽图形为例,对结构DNA纳米技术予以评析与探讨;鉴于该技术目前主要应用于引导无机纳米粒子精确装配、装备纳米生物芯片以及与微加工技术相结合等方面,提出:纳米电子电路及器件、纳米光电子学、高灵敏度高特异性生物传感器、分子机器人、材料学和纳米医学等领域,将是结构DNA纳米技术应用未来的发展方向。

  10. DN A链置换技术的研究现状与展望%Current situation and prospect of DNA strand displacement technology

    Institute of Scientific and Technical Information of China (English)

    姚莉娜; 田桂花; 叶盟盟; 赵涛涛; 崔光照; 王延峰

    2014-01-01

    DNA strand displacement as a new kind of technology has been used in the construction of artifi-cial biochemical logic circuit depending on the DNA molecular component.The research progress of DNA strand displacement technology in many fields was reviewed,including logic gate operation model,bio-chemical logic circuit and neural network,DNA nanorobotics,DNA reaction network and so on.The relat-ed applications of DNA strand displacement technology obtained experimental verification through the de-sign and the simulation of half adder/full adder and encoder logic operation models.Based on these,it was put forward that constructing dynamic and functional nanomachines,integrating DNA logic gates and build-ing DNA computer system structure were to be the development direction of application of DNA strand dis-placement technology.%综述了利用DNA分子元件构造人工逻辑生化电路的新技术---DNA链置换技术在构造逻辑门运算模型、生化逻辑电路与神经网络、DNA纳米机器人、DNA反应网络等领域的研究进展,并通过对半加器/全加器逻辑运算模型和编码器逻辑运算模型的设计及仿真,对DNA链置换技术的相关应用进行了实验验证。在此基础上提出:构建运动及功能型DNA纳米机器,整合DNA逻辑门、自底向上地构建DNA计算机体系结构,将是DNA链置换技术应用的发展方向。

  11. [Physicians see both pros and cons of health care financial management. Questionnaire study provides more insights--with starting point in controversial DN-article series].

    Science.gov (United States)

    Björk, Joar; Petersson, Christer

    2015-05-12

    In the spring of 2013, the Swedish journalist Maciej Zaremba wrote a series of articles criticizing the impact of NPM (New Public Management) on Swedish health care. The present study examines the views of experienced Swedish physicians (general practitioners and internal medicine speclialists) on the problems focused in Mr Zaremba's article series. The respondents (51 general practitioners and 61 internal medicine specialists) mention advantages as well as disadvantages with NPM in Swedish health care. The majority agrees that with NPM, physicians loose influence over health care governance to other professional groups. The majority disagree with the charge made by Mr Zaremba that NPM has had the effect of manipulating Swedish physicians away from the standards of good medical care.

  12. Measurement of the centrality dependence of the charged particle $dn/d\\eta$ in p+Pb at 5.02 TeV

    CERN Document Server

    Cole, B; The ATLAS collaboration; Milov, A; Perepelitsa, D

    2014-01-01

    Proton-lead collisions at the LHC provide an opportunity to probe the physics of the initial state of ultra-relativistic heavy ion collisions. In particular, they can provide insight on the effect of an extended nuclear target on the dynamics of soft and hard scattering processes and subsequent particle production. Charged particle multiplicity and pseudorapidity distributions are among the most basic experimental probes of particle production. The centrality dependence of the charged particle pseudorapidity distributions, dNch/deta , was measured in p+Pb collisions at a nucleon-nucleon centre-of-mass energy of sqrt(s_NN) = 5.02 TeV using the ATLAS detector. Charged particles were reconstructed over |eta| < 2.7 using the ATLAS pixel detector. The proton-lead collision centrality was characterized by the total transverse energy measured over the pseudorapidity interval 3.2 < eta < 4.9 in the direction of the lead beam. The dNch/deta distributions are found to vary strongly with centrality, with an inc...

  13. Propojení přírodovědného a environmentálního vzdělávání

    NARCIS (Netherlands)

    Wals, A.E.J.; Brody, M.; Dillon, J.; Stevenson, R.B.

    2015-01-01

    What is written about in Science journal? A paraphrase of the article: Wals, Arjen E. J., Michael Brody, Justin Dillon, Robert B. Stevenson (2014). Convergence Between Science and Environmental Education. Science, 344(6184), 583–584. DOI:10.1126/science.1250515

  14. cDNA library Table: BmP [KAIKOcDNA[Archive

    Lifescience Database Archive (English)

    Full Text Available BmP NA Qingsong-Haoyue whole body pupal stage mixed pHelix Site_1 Hind II M13 Forwa...rd DN236876-DN237878, DN591076-DN591089,DN985172-DN985373,DY230449-DY231514 EST[number] whole pupae body but for the skin ...

  15. Proměna postojů k lidským právům v Evropě: Pohled českých nevládních organizací

    OpenAIRE

    Libová, Anna

    2017-01-01

    This master's thesis deals with the identification and the analysis of the changes in society which lead to questioning the basic principles of human rights from the point of view of Czech non-profit organisations. In this study, it is important to divide the definition of human rights into their legal aspect, i.e. as rights of people, and their institutional aspect, i.e. as the moral imperative of equality and liberty. The latter is the main objective of this study, and is in this thesis lab...

  16. Correlation of sperm DNA fragmentation index and semen parameters%精子DN A碎片指数与精液参数的相关性研究

    Institute of Scientific and Technical Information of China (English)

    秦文松; 刘英; 段金良

    2014-01-01

    目的:探讨男性精子DNA碎片与精液常规参数及精子顶体酶活性之间的关系。方法:收集生殖中心门诊553例男性患者精液标本,采用改良精子染色质扩散实验(SCD)检测精子 DNA 碎片指数(DFI),按精子DFI结果分为DFI<15%(I组)、15%≤DFI<25%(II组)、DFI≥25%(III组),同时检测精子密度、总数、(a+b)级精子百分率、畸形率及顶体酶活性,对DFI与精液各项参数的相关性进行分析。结果:精子密度及精子总数与DFI无显著相关性;前向运动精子百分率随DFI升高逐渐降低,III组与I组间存在显著性差异;精子正常形态率随DFI的增加有下降的趋势,但是差异不显著;精子顶体酶活性随DFI升高而降低,组间均有显著性差异。结论:精子DNA碎片指数与前向精子百分率及精子顶体酶活性呈负相关。因此DFI可作为评估精子功能的一个较好的参考指标。%Objectives:To explore the relationship between the male sperm DNA fragmentation,semen pa-rameters and acrosome activity.Method:Semen samples of 553 male patients were collected from our center. Sperm DNA fragmentation index(DFI)was detected by Sperm Chromatin Dispersion(SCD)methods.According to the results of sperm DFI,all the values were divided into group I (DFI<15%),group II (15%≤DFI <25%) and group III (DFI≥25%),and the sperm density,count,percentage of progressive motility sperm,morphology and acrosome activity were detected at the same time.The correction of DFI and semen parameters were analyzed. Results:Sperm density and total sperm count had no significant correlation with the DFI.The percentage of progres-sive motility sperm gradually decreased with the augment of DFI,and there were significant differences between group I and group III.Normal sperm morphology rate also decreased with the augment of DFI,and the difference was not significant.Acrosome activity gradually decreased with the augment of DFI as well,with significant differ-ences among the groups.Conclusion:Negative correlations were obtained between both progressive motility sperm and acrosome activity.As a result,DFI is a better reference index for sperm function.

  17. Vyhledávání mimoř ádných prožitků a preference učebního stylu studenta. / Sensation seeking and student’s preferred learning style.

    Directory of Open Access Journals (Sweden)

    Dita Culková

    2013-06-01

    Full Text Available The objective of this empirical study is to determine what learning style students and how this relates to their sensation seeking tendency. We were also interested in what natural clusters occur if these two variables are considered, together with gender and students’ affiliation with general or physical education oriented class at grammar school. Theoretical basis, which are Dunn’s theory of learning styles and theory of Zuckerman’s sensation seeking tendency, are introduced in the first part. The second part describes used methods including reliability and validity verification of the applied questionnaire. Cluster analysis is the method for data processing and provides a deep insight into a big amount of variables. The third part informs about the main results, discusses them and provides praxis recommendations. The greatest differences between genders and class type were found in sensation seekingtendency, partly in learning styles. Girls of physical education and sport specialized classes least represent cluster, where there is a very little thrill and adventure seeking and seeking of new experiences, where boredom susceptibility is low and respecttowards rules and norms is high. Boys are most often members of a cluster where thrill and adventure is not searched but new experiences are welcome, enjoyment follows from disrespect for norms and standards and the members are susceptible to boredom. Boys of physical education and sport classes are little responsible withinthe learning process, they need structured tasks and they prefer experiential or kinesthetic education. It is possible to state, that the preference of learning style of grammar school students might relate to sensation seeking tendency as well as to gender and type of school class.

  18. Diagnóstico de las dificultades de lectura y escritura y de la dislexia basado en la Teoría PASS de lainteligencia utilizando la batería DN-CAS. Origen cognitivo de la dislexia

    OpenAIRE

    Timoneda Gallart, Carme; Pérez Álvarez, Frederic; Mayoral, Sílvia; Serra Sala, Mireia

    2013-01-01

    Las dificultades de lectura y escritura se pueden detectar desde el momento en que los niños y niñas inician el aprendizaje de la lectoescritura en la etapa de Educación Infantil. En algunos casos estos alumnos reciben un apoyo escolar que en muchas ocasiones no conlleva las mejoras esperadas, siendo las técnicas y metodologías de refuerzo aplicadas ineficaces. El problema, desde nuestro punto de vista, empieza con el diagnóstico que se realiza a estos jóvenes, que determina las directrices d...

  19. 广电 EPON +FTTB 网络O DN 光缆纤芯规划设计%The Design Project of ODN Optical Fiber Core in Broadcasting & Television EPON+FTTB Network

    Institute of Scientific and Technical Information of China (English)

    刘春元

    2015-01-01

    FTTx and PON technic is the mainstream technology of 2-ways access network in telecom updating/Broadcasting&Television system bidirectional rebuilding/Next Generation Broadcasting( NGB) at present.The ODN of PON system will cast much, to start it need foresight, the project of ODN structure and optical fiber quantity should give full consideration to future FTTH request.Therefore proposed EPON +FTTB typical structure of ODN fiber core programming design method for Broadcasting & Television, to simple analyses ODN split fiber structure in PON system.%FTTx及PON技术是当前电信升级、广电双改及下一代广播电视网络( NGB )双向接入网的主流技术。 PON系统的ODN部分投入较大,ODN的建设要有前瞻性,ODN结构及其光缆纤芯规划需充分考虑今后能否满足FTTH的需求。为此提出了广电EPON+FTTB这一典型结构的ODN纤芯规划设计方法,并对PON系统ODN的分光结构进行分析。

  20. A magyar családnévanyag kontrasztív szempontú tipológiai-statisztikai vizsgálata [Contrastive typological-statistical analysis of the Hungarian family name stock

    Directory of Open Access Journals (Sweden)

    Farkas, Tamás

    2016-12-01

    Full Text Available The comprehensive description of a country’s family name stock has been, and is being, carried out in several European countries, including Hungary. The paper, from among other possible lines of research, offers a typological-statistical survey of the Hungarian family name stock. The study focuses on the 100 most frequent family names of contemporary Hungary, while taking international research (European Surname Typology Project into account and drawing its conclusions from complete or highly representative name corpora. The paper discusses the typological distribution of the 100 most frequent contemporary family names in Hungary, in comparison with Hungarianhistorical family names from the beginning of the 18th century, as well as types of art ificial Hungarian family names produced in the processes of official family name changes in the 19–20th centuries. Certain peculiarities of Hungarian family names are also pointed out when compared to the family names of other languages and countries. The paper also focuses on some questions of research methodology that arise in the field and are rarely discussed in relevant international literature. Different approaches in research methodology are described with their consequences, i.e. differences in research results. Ultimately, the paper intends, in part, to give a picture of the Hungarian family name stock and the possibilities of further research in the field, while also exposing a number of ideas that should also be taken into consideration by international research projects of great importance.

  1. Marketingová komunikace módních značek a jejich vliv na vnímání body image spotřebitele

    OpenAIRE

    Macková, Monika

    2012-01-01

    The Master's Thesis deals with the marketing communication of fashion industry with the focus on two particular global fast fashion brands. The main goal is to identify the specifics of marketing communication strategies of fashion industry, to analyze the approach difference of the two chosen fashion brands H&M and Zara and to define their influence on consumer's body image through qualitative and quantitative research. The thesis is divided into several parts. The first chapter follows up t...

  2. Array-based comparative genomic hybridization facilitates identification of breakpoints of a novel der(1)t(1;18)(p36.3;q23)dn in a child presenting with mental retardation.

    Science.gov (United States)

    Lennon, P A; Cooper, M L; Curtis, M A; Lim, C; Ou, Z; Patel, A; Cheung, S W; Bacino, C A

    2006-06-01

    Monosomy of distal 1p36 represents the most common terminal deletion in humans and results in one of the most frequently diagnosed mental retardation syndromes. This deletion is considered a contiguous gene deletion syndrome, and has been shown to vary in deletion sizes that contribute to the spectrum of phenotypic anomalies seen in patients with monosomy 1p36. We report on an 8-year-old female with characteristics of the monosomy 1p36 syndrome who demonstrated a novel der(1)t(1;18)(p36.3;q23). Initial G-banded karyotype analysis revealed a deleted chromosome 1, with a breakpoint within 1p36.3. Subsequent FISH and array-based comparative genomic hybridization not only confirmed and partially characterized the deletion of chromosome 1p36.3, but also uncovered distal trisomy for 18q23. In this patient, the duplicated 18q23 is translocated onto the deleted 1p36.3 region, suggesting telomere capture. Molecular characterization of this novel der(1)t(1;18)(p36.3;q23), guided by our clinical array-comparative genomic hybridization, demonstrated a 3.2 Mb terminal deletion of chromosome 1p36.3 and a 200 kb duplication of 18q23 onto the deleted 1p36.3, presumably stabilizing the deleted chromosome 1. DNA sequence analysis around the breakpoints demonstrated no homology, and therefore this telomere capture of distal 18q is apparently the result of a non-homologous recombination. Partial trisomy for 18q23 has not been previously reported. The importance of mapping the breakpoints of all balanced and unbalanced translocations found in the clinical laboratory, when phenotypic abnormalities are found, is discussed.

  3. Dětské čtenářství v didaktických souvislostech a perspektivy neurovědních poznatků. / Didactic background of children’s reading and perspectives of neuroscience.

    Directory of Open Access Journals (Sweden)

    Václav Jindráček

    2013-10-01

    Full Text Available The goal of this review is to look up research studies, that have by using the functional or electrophysiological methods investigated the activation of cortical parts of brain during reading or narrating astory (narrative or discourse, and by using reviews give the overview of the results which the selected works achieved. The first part of the report is devoted to the contemporary children reading in educational contexts in literary education. In the second part an overview of research studies mapping the activation of cortical parts of the brain during the process of reading and the understanding of narrative/discourse is provided. As a source of data, thefollowing databases were used (2007–2012: Medline, Bibliomedica, Science Direct, Web of Science, Google Scholar. 16 studies were included in the analysis. It has come to light, that the identified works considered the specifics of pubescent and prepubescent readers only in few isolated cases, yet in spite of that, gave extensiveoverview about the options that can be used to clarify the mechanisms of the process of reading a narrative text on the level of cortical activation of the brain. Sixteen research studies were included in the analysis. In the evaluated articles, great importance hasbeen given to the research of activity of brain parts/hemispheres in the process of construction of the so called situational models (during the segmentation of the narrative into individual parts/events and defining contrasts that characterize neural correlates of inferential processes.

  4. Multibeam collection for KRY11_06: Multibeam data collected aboard Keary from 2011-09-20 to 2011-10-04, departing from Clogherhead, Ireland and returning to D?n Laoghaire, Ireland

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  5. Multibeam collection for KRY12_01: Multibeam data collected aboard Keary from 2012-04-13 to 2012-06-04, departing from D?n Laoghaire, Ireland and returning to Clogherhead, Ireland

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  6. Duloxetine and pregabalin: high-dose monotherapy or their combination? The "COMBO-DN study"--a multinational, randomized, double-blind, parallel-group study in patients with diabetic peripheral neuropathic pain.

    Science.gov (United States)

    Tesfaye, Solomon; Wilhelm, Stefan; Lledo, Alberto; Schacht, Alexander; Tölle, Thomas; Bouhassira, Didier; Cruccu, Giorgio; Skljarevski, Vladimir; Freynhagen, Rainer

    2013-12-01

    This multicentre, double-blind, parallel-group study in diabetic peripheral neuropathic pain addressed whether, in patients not responding to standard doses of duloxetine or pregabalin, combining both medications is superior to increasing each drug to its maximum recommended dose. For initial 8-week therapy, either 60 mg/day duloxetine (groups 1, 2) or 300 mg/day pregabalin (groups 3, 4) was given. Thereafter, in the 8-week combination/high-dose therapy period, only nonresponders received 120 mg/day duloxetine (group 1), a combination of 60 mg/day duloxetine and 300 mg/day pregabalin (groups 2, 3), or 600 mg/day pregabalin (group 4). Primary outcome (Brief Pain Inventory Modified Short Form [BPI-MSF] 24-hour average pain change after combination/high-dose therapy) was analyzed comparing combination (groups 2, 3 pooled) with high-dose monotherapy (groups 1, 4 pooled). Secondary end points included response rates, BPI-MSF severity items, and comparison of duloxetine and pregabalin in BPI-MSF average pain. Eight hundred four patients were evaluated for initial therapy and 339 for combination/high-dose therapy. There were no significant differences between combination and high-dose monotherapy regarding BPI-MSF average pain (mean change: combination: -2.35; high-dose monotherapy: -2.16; P = 0.370) and most secondary end points, which, however, consistently favoured combination therapy. Fifty-percent response rates were 52.1% for combination and 39.3% for high-dose monotherapy (P = 0.068). In exploratory analyses of the initial 8-week therapy uncorrected for multiple comparisons, 60 mg/day duloxetine was found superior to 300 mg/day pregabalin (P < 0.001). Both drugs and their combination were well tolerated. Although not significantly superior to high-dose monotherapy, combination therapy was considered to be effective, safe, and well tolerated. Crown Copyright © 2013. Published by Elsevier B.V. All rights reserved.

  7. Multibeam collection for CV1202: Multibeam data collected aboard Celtic Voyager from 2012-02-01 to 2012-02-28, departing from Cork, Ireland and returning to D?n Laoghaire, Ireland

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  8. Multibeam collection for cb12_01: Multibeam data collected aboard Cosantoir Bradan from 2012-09-17 to 2012-10-07, departing from D?n Laoghaire, Ireland and returning to Cork, Ireland

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is part of a larger set of data called the Multibeam Bathymetry Database (MBBDB) where other similar data can be found at...

  9. 19q13.11 microdeletion concomitant with ins(2;19)(p25.3;q13.1q13.4)dn in a boy: potential role of UBA2 in the associated phenotype.

    Science.gov (United States)

    Venegas-Vega, Carlos; Nieto-Martínez, Karem; Martínez-Herrera, Alejandro; Gómez-Laguna, Laura; Berumen, Jaime; Cervantes, Alicia; Kofman, Susana; Fernández-Ramírez, Fernando

    2014-01-01

    The 19q13.11 microdeletion syndrome (MIM613026) is a clinically recognisable condition in which a 324-kb minimal overlapping critical region has been recently described. However, genes not included within this region, such as WTIP and UBA2, have been proposed to contribute to the clinical characteristics observed in patients. Using cytogenetic techniques, single nucleotide polymorphism arrays, and the quantitative polymerase chain reaction, we identified a novel case with a 2.49-Mb deletion derived from a de novo chromosomal rearrangement. Based on a review of the literature, we support the notion that UBA2 haploinsufficiency could contribute to the phenotype of this rare genomic disorder. UBA2 belongs to a protein complex with sumoylation activity, and several transcription factors, hormone receptors, and signalling proteins related to brain and sexual development are regulated by this post-translational modification. Additional clinical reports and further research on UBA2 molecular function are warranted.

  10. DNA immobilization and hybridization on graphene/gold nanoparticles/polypyrrole nanocomposite%DN A在石墨烯/金纳米/聚吡咯复合材料上的固定及杂交

    Institute of Scientific and Technical Information of China (English)

    王明花; 杨光; 张园厂; 康萌萌; 何领好; 冯孝中; 彭东来; 张治红

    2014-01-01

    采用原位化学氧化聚合法制备石墨烯/金纳米/聚吡咯(G/AuNPs/PPy)纳米复合材料,并在其表面进行DNA的固定及杂交.使用傅里叶变换红外光谱、X-射线光电子能谱、场发射扫描电子显微镜及透射电子显微镜对复合材料的化学结构、元素组成和表面形貌进行表征,DNA固定及杂交前后复合材料电化学性能和质量的变化运用电化学循环伏安、交流阻抗和石英晶体微天平等方法测试,结果表明,G/AuNPs/PPy复合材料电化学性能明显优于G/AuNPs二元材料,且DNA在复合材料表面的固定量可达307 ng,同时能检测到357 ng完全匹配靶向DNA.%Graphene/gold nanoparticles/polypyrrole (G/AuNPs/PPy)nanocomposite was prepared by in-situ chemical oxidation method,then DNA was immobilized and hybridized on the surface of the composite. The chemical structure,element composition and surface morphology of the nanocomposite were character-ized by Fourier transform infrared spectroscopy,X-ray photoelectron spectroscopy,field emission scanning electron microscope and transmission electron microscopy,respectively.Afterwards,electrochemical cyclic voltammetry,impedance spectroscopy and electrochemical quartz crystal microbalance were used to deter-mine the variation of electrochemical properties and weight of the nanocomposite before and after DNA im-mobilization/hybridization.It demonstrated that the electrochemical activities of G/AuNPs/PPy were better than that of G/AuNPs nanocomposite.The immobilization amount of DNA on the G/AuNPs/PPy nanocom-posite was 307 ng,and aslo 357 ng of perfectly matched target DNA was detected on the surface.

  11. Increased axonal regeneration and swellings in intraepidermal nerve fibers characterize painful phenotypes of diabetic neuropathy

    OpenAIRE

    Cheng, H.T.; Dauch, J.R.; Porzio, M.T.; Yanik, B.M.; Hsieh, W; Smith, A.G.; Singleton, J.R.; Feldman, E.L.

    2013-01-01

    We examined changes in intraepidermal nerve fibers (IENFs) to differentiate patients with diabetic neuropathy (DN) and neuropathic pain (DN-P) from those with DN without pain (DN-NOP). Punch skin biopsies were collected from the proximal thigh (PT) and distal leg (DL) of normal subjects (NS), patients with type 2 diabetes without evidence of DN (DM), or DN-P and DN-NOP patients. Protein gene product 9.5 (PGP) immunohistochemistry was used to quantify total IENF, and growth associated protein ...

  12. A Matched Filter Algorithm for Acoustic Signal Detection.

    Science.gov (United States)

    1985-06-01

    16 DNL(4),OQ(4),DN21(2),DD21(2),DN22(2),DD22(2) A8P00690 COMPLEX DNL5(5), DD45 (5),DN213(3),DD213(3),DN223(3),DD223(3) ABPOO700 C TABULATED INPUT... DD45 (5)=CMPLX(l.,0.) ABPO04030 DO 512 1=1,4 ABPO40L40 DN45( I)=DN4( I) ABPO4050 0045(1 )=D04( I) ABPO4060 512 CONTINUE ABP04070 WRITE(4,514) ABP0J4080...DN45(4),DN45(3),DN45(2),DN45( 1) ABP04120 WRITE(4,530) DD45 (5),D045(4), DD45 (3), DD45 (2), DD45 (l) ABP04130 515 FORMAT(/’ SINGLE FOURTH ORDER TRANSFER

  13. UAS Collision Avoidance Algorithm that Minimizes the Impact on Route Surveillance

    Science.gov (United States)

    2009-03-01

    initialize cone variables as empty acount =[]; alpha_up=[];alpha_dn=[];alpha_up_dot=[];alpha_dn_dot=[]; acountv=[]; alpha_upv=[];alpha_dnv=[];alpha_up_dotv...8217 + phidot(kk)]; alpha_dn_dotv = [alpha_dn_dotv; eta_dn_dotv’ + phidot(kk)]; %Record the number of cones acount ... acount length(eta_up)]; acountv = [acountv length(eta_upv)]; end end violation = max(violation1); %Check and correct for horizontal

  14. Estudo termoanalítico de compatibilidade fármaco-excipiente e de estabilidade entre ácido lipóico e adjuvantes tecnológicos

    OpenAIRE

    Silva,Paulo César Dantas da

    2014-01-01

    O ácido lipóico (AL) é um antioxidante de ocorrência endógena e exógena que atua como um cofator essencial em complexos multienzimáticos como: α-cetoglutarato desidrogenase, piruvato desidrogenase, complexo α-cetoácido desidrogenase e o complexo glicina descarboxilase. Quatro propriedades terapêuticas desta molécula já foram bastante estudadas: capacidade de quelar íons metálicos como: Cd 2+ , Fe 3+ , Cu 2+ e Zn 2+ , reter espécies reativas ao oxigênio ...

  15. Knock-in gene correction of induced pluripotent stem cells from pyruvate kinase deficient patient

    OpenAIRE

    Garate Mutiloa, Zita

    2014-01-01

    Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 27-11-2013 La Deficiencia en Piruvato Quinasa (DPQ) es una enfermedad rara causada por mutaciones en el gen PKLR que provoca Anemia Hemolítica no Esferocítica Crónica (AHNEC). El único tratamiento definitivo para los casos graves de DPQ es el Trasplante Alogénico de Médula Ósea (TAMO). Debido a los riesgos asociados a TAMO, como la enfermedad de injer...

  16. Knock-in gene correction of induced pluripotent stem cells from pyruvate kinase deficient patient

    OpenAIRE

    Garate Mutiloa, Zita

    2013-01-01

    Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 27-11-2013 La Deficiencia en Piruvato Quinasa (DPQ) es una enfermedad rara causada por mutaciones en el gen PKLR que provoca Anemia Hemolítica no Esferocítica Crónica (AHNEC). El único tratamiento definitivo para los casos graves de DPQ es el Trasplante Alogénico de Médula Ósea (TAMO). Debido a los riesgos asociados a TAMO, como la enfermedad de injer...

  17. Prevalência de mycobacterium bovis e estudo dos fatores associados entre casos de tuberculose atendidos em centros de referência, Brasil, Março 2008 Fevereiro 2010

    OpenAIRE

    Marcio Roberto Silva

    2011-01-01

    Introdução: Mycobacterium bovis é um agente do complexo M. tuberculosis que causa TB animal, mas que pode afetar também o ser humano, sendo a sua contribuição relativa no contexto global da tuberculose humana (TB) provavelmente subestimada devido às suas características de crescimento disgônico e lento e, à ausência de piruvato na maioria dos meios utilizados que dificulta o seu isolamento primário. No Brasil, cultivos de microrganismos do complexo Mycobacterium tuberculosis (CMT), sua identi...

  18. Optimum design of sustainable sealed low volume roads using the Dynamic Cone Penetrometer (DCP)

    CSIR Research Space (South Africa)

    Paige-Green, P

    2012-09-01

    Full Text Available into the gravel or soil material (DN in mm/blow) has been found to be a reasonably good predictor of the California Bearing Ratio (CBR) at the prevailing in situ moisture and density conditions using the following equations (Kleyn 1984) shown diagrammatically... in Figure 2: If DN > 2 mm/blow CBR = 410 x DN-1.27 (1) If DN < 2 mm/blow CBR = (66.66 x DN2) - (330 x DN) + 563.33 (2) The first equation is generally more applicable to lower traffic roads with in situ CBR values up to about 150%. Of major significance...

  19. Dicty_cDB: SHA342 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available . 38 0.97 3 DN203564 |DN203564.1 USDA-FP_139589 5th Instar Glassy-winged Sharpshooter Homalodisca coagulata ...cDNA clone WHHC5-019_G02 5', mRNA sequence. 46 1.0 1 DN203476 |DN203476.1 USDA-FP_139501 5th Instar Glassy-w...ce. 46 1.0 1 DN203471 |DN203471.1 USDA-FP_139496 5th Instar Glassy-winged Sharpshooter Homalodisca coagulata... cDNA clone WHHC5-015_C06 5', mRNA sequence. 46 1.0 1 DN203427 |DN203427.1 USDA-FP_139452 5th Instar Glassy-...03388.1 USDA-FP_139413 5th Instar Glassy-winged Sharpshooter Homalodisca coagulat

  20. 77 FR 19032 - Certain Semiconductor Integrated Circuit Devices and Products Containing Same Notice of Receipt...

    Science.gov (United States)

    2012-03-29

    ...] [FR Doc No: 2012-7567] INTERNATIONAL TRADE COMMISSION [DN 2888] Certain Semiconductor Integrated... Certain Semiconductor Integrated Circuit Devices and Products Containing Same, DN 2888; the Commission is... importation of certain semiconductor integrated circuit devices and products containing same. The...

  1. JMBR APRIL.cdr

    African Journals Online (AJOL)

    FinePrint

    Diabetic nephropathy (DN) is the leading cause of end-stage renal ... Twenty-two newly-diagnosed DN patients due to type2 diabetes mellitus(8 males ... Complications Trial (DCCT) and United ..... of macrovascular and microvascular.

  2. The effects of blocking angiotensin receptors on early stages of diabetic nephropathy

    Directory of Open Access Journals (Sweden)

    Alaleh Gheissari

    2012-01-01

    Conclusion: Angiotensin receptor blockers may reduce VCAM-1 and microalbuminuria and may increase NO levels in early stages of DN. Thus administration of ARBs might be considered even in early stages of DN.

  3. Early systemic microvascular damage in pigs with atherogenic diabetes mellitus coincides with renal angiopoietin dysbalance

    NARCIS (Netherlands)

    M. Khairoun (Meriem); M.M. van den Heuvel (Mieke); B. van den Berg (Bernard); O. Sorop (Oana); De Boer, R. (Rients); N.S. van Ditzhuijzen (Nienke); I.M. Bajema (Ingeborg); H.J. Baelde; M. Zandbergen; D.J.G.M. Duncker (Dirk); T. Rabelink (Ton); M.E. Reinders (Marlies); W.J. van der Giessen (Wim); J.I. Rotmans (Joris)

    2015-01-01

    textabstractBackground: Diabetes mellitus (DM) is associated with a range of microvascular complications including diabetic nephropathy (DN). Microvascular abnormalities in the kidneys are common histopathologic findings in DN, which represent one manifestation of ongoing systemic microvascular dama

  4. Personality and complex brain networks: The role of openness to experience in default network efficiency

    OpenAIRE

    2015-01-01

    Abstract The brain's default network (DN) has been a topic of considerable empirical interest. In fMRI research, DN activity is associated with spontaneous and self‐generated cognition, such as mind‐wandering, episodic memory retrieval, future thinking, mental simulation, theory of mind reasoning, and creative cognition. Despite large literatures on developmental and disease‐related influences on the DN, surprisingly little is known about the factors that impact normal variation in DN functio...

  5. Dicty_cDB: SHG171 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available T10I14 (ESSA project). 38 2.4 2 DN191182 |DN191182.1 ACL5_13E12 AcL5 cDNA Library Angiostrongylus cantonens...is cDNA, mRNA sequence. 44 3.7 1 DN190596 |DN190596.1 ACL5_06E22 AcL5 cDNA Library Angiostrongylus canto

  6. Dicty_cDB: SHC461 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available is thaliana DNA chromosome 4, contig fragment No. 38. 42 1.7 3 DN191182 |DN191182.1 ACL5_13E12 AcL5 cDNA Library Angiostrongylus cant...onensis cDNA, mRNA sequence. 44 3.9 1 DN190596 |DN190596.1 ACL5_06E22 AcL5 cDNA Library Angiostrongylus cant

  7. Dicty_cDB: VFI734 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CLLWSDPQSNPGIAPSSRGVGVYFGPDVTRRFLKEN NLSTIIRSHEVKEKGYQIDDDGSLITVFSAPNYCDQSGNLGSFINITE...VYFGPDVTRRFLKEN NLSTIIRSHEVKEKGYQIDDDGSLITVFSAPNYCDQSGNLGSFINITEDKIKITTLKL*t seyttnalakk Homology vs CSM-cDN

  8. The phenotypes of podocytes and parietal epithelial cells may overlap in diabetic nephropathy

    NARCIS (Netherlands)

    Andeen, Nicole K; Nguyen, Tri Q; Steegh, Floor; Hudkins, Kelly L; Najafian, Behzad; Alpers, Charles E

    2015-01-01

    Reversal of diabetic nephropathy (DN) has been achieved in humans and mice, but only rarely and under special circumstances. As progression of DN is related to podocyte loss, reversal of DN requires restoration of podocytes. Here, we identified and quantified potential glomerular progenitor cells th

  9. Laparoendoscopic single-site nephrectomy in obese living renal donors.

    Science.gov (United States)

    Afaneh, Cheguevara; Sheth, Seema; Aull, Meredith J; Leeser, David B; Kapur, Sandip; Del Pizzo, Joseph J

    2012-02-01

    Laparoendoscopic single-site (LESS) surgery has been shown to be feasible in living donor nephrectomies (DNs). Obesity is an established risk factor for perioperative morbidity. We sought to determine whether LESS-DN is safe and effective in the obese (body mass index [BMI] ≥30 kg/m(2)) population. Between August 2009 and September 2010, 125 consecutive LESS-DN were performed; 32 patients were obese. This group was matched to 32 nonobese LESS-DN (BMI obese conventional laparoscopic DN (obese LAP-DN) patients, and 32 nonobese LAP-DN patients. Comparison parameters included organ recovery time, operative time, estimated blood loss (EBL), warm ischemia time (WIT), incision length, complications, and recipient allograft function. Demographic data were similar between the groups, except BMI (P>0.0001). Organ recovery time, EBL, WIT, complications, and recipient allograft function were similar between the obese LESS-DN group and the other three groups (P>0.05). Total operative time was longer in the obese LESS-DN compared with the nonobese LAP-DN (Pobese LESS-DN group compared with either LAP group (Pobese donor cases were converted to hand-assisted laparoscopy). Our results indicate that LESS-DN can be performed safely in obese donors without increased donor morbidity and similar recipient allograft outcomes compared with ideal-sized donors as well as with conventional LAP-DN patients.

  10. Multicentre prospective validation of a urinary peptidome-based classifier for the diagnosis of type 2 diabetic nephropathy

    NARCIS (Netherlands)

    Siwy, Justyna; Schanstra, Joost P.; Argiles, Angel; Bakker, Stephan J. L.; Beige, Joachim; Boucek, Petr; Brand, Korbinian; Delles, Christian; Duranton, Flore; Fernandez-Fernandez, Beatriz; Jankowski, Marie-Luise; Al Khatib, Mohammad; Kunt, Thomas; Lajer, Maria; Lichtinghagen, Ralf; Lindhardt, Morten; Maahs, David M.; Mischak, Harald; Mullen, William; Navis, Gerarda; Noutsou, Marina; Ortiz, Alberto; Persson, Frederik; Petrie, John R.; Roob, Johannes M.; Rossing, Peter; Ruggenenti, Piero; Rychlik, Ivan; Serra, Andreas L.; Snell-Bergeon, Janet; Spasovski, Goce; Stojceva-Taneva, Olivera; Trillini, Matias; von der Leyen, Heiko; Winklhofer-Roob, Brigitte M.; Zuerbig, Petra; Jankowski, Joachim

    2014-01-01

    Diabetic nephropathy (DN) is one of the major late complications of diabetes. Treatment aimed at slowing down the progression of DN is available but methods for early and definitive detection of DN progression are currently lacking. The 'Proteomic prediction and Renin angiotensin aldosterone system

  11. Multicentre prospective validation of a urinary peptidome-based classifier for the diagnosis of type 2 diabetic nephropathy

    DEFF Research Database (Denmark)

    Siwy, Justyna; Schanstra, Joost P; Argilés, Angel

    2014-01-01

    BACKGROUND: Diabetic nephropathy (DN) is one of the major late complications of diabetes. Treatment aimed at slowing down the progression of DN is available but methods for early and definitive detection of DN progression are currently lacking. The 'Proteomic prediction and Renin angiotensin aldo...

  12. Novel susceptibility locus at 22q11 for diabetic nephropathy in type 1 diabetes

    DEFF Research Database (Denmark)

    Wessman, Maija; Forsblom, Carol; Kaunisto, Mari A

    2011-01-01

    Diabetic nephropathy (DN) affects about 30% of patients with type 1 diabetes (T1D) and contributes to serious morbidity and mortality. So far only the 3q21-q25 region has repeatedly been indicated as a susceptibility region for DN. The aim of this study was to search for new DN susceptibility loci...

  13. The phenotypes of podocytes and parietal epithelial cells may overlap in diabetic nephropathy

    NARCIS (Netherlands)

    Andeen, Nicole K; Nguyen, Tri Q; Steegh, Floor; Hudkins, Kelly L; Najafian, Behzad; Alpers, Charles E

    Reversal of diabetic nephropathy (DN) has been achieved in humans and mice, but only rarely and under special circumstances. As progression of DN is related to podocyte loss, reversal of DN requires restoration of podocytes. Here, we identified and quantified potential glomerular progenitor cells

  14. Clinical Characteristics of Patients with Diabetic Nephropathy on Maintenance Hemodialysis: A Multicenter Cross-sectional Survey in Anhui Province, Eastern China

    Directory of Open Access Journals (Sweden)

    Hu Chen

    2016-01-01

    Conclusions: DN patients on MHD in Anhui province exhibited different clinical characteristics compared to non-DN hemodialysis patients. They presented higher percentage in TCC use and cardiovascular complication, lower serum albumin and iPTH levels than those in non-DN patients.

  15. Porovnání zpracování barevné modifikace Warteggova kresebného testu u studentů psychologie a přírodovědných oborů (bakalářská práce je součástí širšího projektu)

    OpenAIRE

    Klečková, Kateřina

    2012-01-01

    This bachelor thesis compares outcomes of colored modification of Wartegg's drawing test in psychology students and natural science students and considers characteristic traits of psychology students. Theoretical part of the thesis describes projective methods, especially Wartegg's drawing test (WZT) and its colored modification with color symbolism. Empirical part is focused on differences in WZT outcomes of 16 psychology students and 16 natural science students. On the basis of these outcom...

  16. Study on the correlation between Super C-reactive protein and Cystatin C in Early Diagnosis of Type 2 diabetic diabetic nephropathy,DN%超敏C反应蛋白和胱抑素C在2型糖尿病肾病早期诊断的临床意义

    Institute of Scientific and Technical Information of China (English)

    王卫; 龙艳; 苏珂; 胡璟; 黄漓莉

    2013-01-01

    目的 探讨血超敏C反应蛋白(Hs-CRP)和胱抑素C(CysC)水平的变化与T2DM诊断的临床意义.方法 将T2DM患者以24小时尿白蛋白排泄率(UAER)水平予分为单纯的T2DM组(I组),早期DN组(II组),分别测定各组间患者血Hs-CRP、CysC、尿素氮(BUN)、肌酐(Cr)以及UAER的浓度,并且与对照组(III组)进行比较.结果 单纯的T2DM组、DN早期组中的血Hs-CRP、CysC水平明显高于对照组,统计学分析有差异(P<005).DN早期组中的血Hs-CRP、CysC水平明星高于单纯的T2DM组(P<005).随着DN病情的进展,血Hs-CRP、CysC水平显著升高(P<0.05).结论 在DN的发生发展中,血Hs-CRP、CysC浓度变化水平与DN患者病情成正比,可作为诊断DN的早期敏感、可靠的常规性指标.因此,在DN早期,血Hs-CRP、CysC可为诊断及监测病变进展提供一定的临床价值.

  17. Dimenzionális szemlélet a névkutatásban. Általános kérdések és a családnévkutatás példája [The dimensional approach in Onomastics. General questions and the example of family-name research

    Directory of Open Access Journals (Sweden)

    Farkas, Tamás

    2014-12-01

    Full Text Available An essential characteristic of natural and modern languages and language use is variability and changeability. These features are connected to the basic existential dimensions of human beings and languages. These dimensions are those of time (historicity, space (spatiality and society (society and culture. Dimensional linguistics as an approach and research area, by considering and examining the above dimensions jointly, seeks to contribute to a better knowledge and understanding of several language phenomena. As a result of the fact that proper names, in comparison with common nouns, are strongly embedded in extralinguisitic reality, their examination should rely on this approach. This paper presents how typical it is to pay attention to the different dimensions in the analyses of the distinct types of names. The author explores the reasons for, the antecedents of, and the presence of this approach in Hungarian Onomastics, focusing on topics such as name history, name geography and socioonomastics as well as their connections and their possible dimensional linguistic examination. Examples are given from the earlier and contemporary literature in the field of Hungarian family-name research. The author argues that most questions in relation to Onomastics can only be discussed validly in the framework of macrolinguistics, using the methods of contextual linguistics.

  18. Analýza užívaných citačních stylů v publikacích autorů z lékařských, sportovních a přírodovědných oborů na Masarykově univerzitě

    OpenAIRE

    Kratochvíl Jiří

    2014-01-01

    Příspěvek představuje výsledky analýzy citačních stylů, podle kterých citovali autoři z vybraných fakult Masarykovy univerzity. Autoři z lékařské fakulty citovali nejčastěji podle stylu Vancouver/NLM (32, 42 % případů) nebo ISO 690 (12, 71 %), autoři z fakulty sportovních studií podle stylu APA (38,24 %), ISO 690 (6,86 %) nebo Vancouver/NLM (5,39 %) a autoři z přírodovědecké fakulty podle vlastního stylu časopisu (46,60 %). Některý z obecně známých stylů následovali jen autoři z oboru astrofy...

  19. “El libro que contiene onze partidos del M. Dn Joseph de Torres”. ¿Torres y Martínez Bravo, o Torres y Vergara? Aproximaciones a una interpretación filológica del manuscrito de la colección Sánchez Garza

    Directory of Open Access Journals (Sweden)

    Delgado Parra, Gustavo

    2008-12-01

    Full Text Available The Torres Book from the Sanchez Garza collection is one of the most polemic issues in the study of Hispanic music. This controversy has been the result of two main factors. On the one hand, the authorship problems and on the other, the apparent disorder of the musical content in this manuscript due to the ineffi cient work done by the copiers who gave it form. The latter has lead to conclude, as a generalized opinion, that it is a highly corrupted copy of the organ works that could have been composed by the famous Spanish composer, organist, musical theorist and printer, Joseph de Torres y Martinez Bravo (*1670c; †1738; in which case the copier would be the one to blame for the demerit of such a renowned musician of the Royal Chapel of Madrid. If to this we add the transcription offered by the CENIDIM in 1993 which multiplies the confusion, we are facing pieces of music full of questions and a musical text which is neither effective nor convincing.El Libro de Torres de la colección Sánchez Garza, es uno de los documentos más polémicos de los últimos tiempos en la musicología hispánica. Esta polémica ha sido el resultado de dos factores fundamentales: por un lado el problema de la autoría, y por el otro, el aparente desorden del contenido musical del manuscrito, debido supuestamente, al trabajo inefi ciente de los copistas que le dieron forma. Esto último ha llevado a concluir, como opinión generalizada, que se trata de una copia corrompida de las obras para órgano que habrían sido compuestas por el prestigiado compositor, organista, teórico musical e impresor español, Joseph de Torres y Martínez Bravo (*1670c; †1738, en cuyo caso el copista del manuscrito sería el responsable de dicho desorden; lo que ha llevado a suponer, a otros investigadores, que incluso las obras en cuestión no sean de su autoría. A esto debemos agregar la confusa y a veces errónea transcripción ofrecida por el CENIDIM en 1993, en cuyo caso las obras han sido adjudicadas a José de Torres y Vergara, un personaje mexicano, homónimo y contemporáneo de Torres y Martínez Bravo.

  20. Rates and determinants of progression to graft failure in kidney allograft recipients with de novo donor-specific antibody.

    Science.gov (United States)

    Wiebe, C; Gibson, I W; Blydt-Hansen, T D; Pochinco, D; Birk, P E; Ho, J; Karpinski, M; Goldberg, A; Storsley, L; Rush, D N; Nickerson, P W

    2015-11-01

    Understanding rates and determinants of clinical pathologic progression for recipients with de novo donor-specific antibody (dnDSA), especially subclinical dnDSA, may identify surrogate endpoints and inform clinical trial design. A consecutive cohort of 508 renal transplant recipients (n = 64 with dnDSA) was studied. Recipients (n = 388) without dnDSA or dysfunction had an eGFR decline of -0.65 mL/min/1.73 m(2) /year. In recipients with dnDSA, the rate eGFR decline was significantly increased prior to dnDSA onset (-2.89 vs. -0.65 mL/min/1.73 m(2) /year, p < 0.0001) and accelerated post-dnDSA (-3.63 vs. -2.89 mL/min/1.73 m(2) /year, p < 0.0001), suggesting that dnDSA is both a marker and contributor to ongoing alloimmunity. Time to 50% post-dnDSA graft loss was longer in recipients with subclinical versus a clinical dnDSA phenotype (8.3 vs. 3.3 years, p < 0.0001). Analysis of 1091 allograft biopsies found that dnDSA and time independently predicted chronic glomerulopathy (cg), but not interstitial fibrosis and tubular atrophy (IFTA). Early T cell-mediated rejection, nonadherence, and time were multivariate predictors of IFTA. Independent risk factors for post-dnDSA graft survival available prior to, or at the time of, dnDSA detection were delayed graft function, nonadherence, dnDSA mean fluorescence intensity sum score, tubulitis, and cg. Ultimately, dnDSA is part of a continuum of mixed alloimmune-mediated injury, which requires solutions targeting T and B cells.

  1. Tissue Culture Technology for Populus × euramericana Hybrids: Effect of Genotype on in Vitro Regeneration

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    In order to select a suitable genotype for the studies on genetic transformation, the difference among three genotypes (DN04-18, DN04-25 and DN04-26) of Populus × euramericana (Populus deltoides × P. nigra) in terms of shoot regeneration was investigated in this paper. An efficient in vitro micropropagation method was successfully established for genotype DN04-18. The high frequency shoot regeneration (90.0%) from leaf discs was obtained in genotype DN04-18 on the modified Murashige and Skoog (mMS) medium w...

  2. Prevalence of diabetic nephropathy among Type 2 diabetic patients in some of the Arab countries

    Science.gov (United States)

    Aldukhayel, Abdulrhman

    2017-01-01

    Type 2 diabetes mellitus (DM) is a public health concern worldwide and an important cause of morbidity and mortality. Type 2 DM is associated with microvascular and macrovascular complications. Diabetic nephropathy (DN), which is characterized by proteinuria, is one of the most serious long-term microvascular complications of DM. The proportion of DN is increasing worldwide. DN is the leading cause of chronic kidney diseases and end-stage renal disease, which constitutes the major workload of dialysis centers worldwide. Microalbuminuria (MA) is the earliest sign of DN, so the early detection of MA and early control of diabetes retards the progression of DN. PMID:28293155

  3. Emerging therapeutics for the treatment of diabetic nephropathy.

    Science.gov (United States)

    Brenneman, Jehrod; Hill, Jon; Pullen, Steve

    2016-09-15

    Diabetic nephropathy (DN) is the most common pathology contributing to the development of chronic kidney disease (CKD). DN caused by hypertension and unmitigated inflammation in diabetics, renders the kidneys unable to perform normally, and leads to renal fibrosis and organ failure. The increasing global prevalence of DN has been directly attributed to rising incidences of Type II diabetes, and is now the largest non-communicable cause of death worldwide. Despite the high morbidity, successful new treatments for DN are lacking. This review seeks to provide new insight on emerging clinical candidates under investigation for the treatment of DN.

  4. Evaluating Kolmogorov\\'s Distribution

    Directory of Open Access Journals (Sweden)

    George Marsaglia

    2003-11-01

    Full Text Available Kolmogorov's goodness-of-fit measure, Dn , for a sample CDF has consistently been set aside for methods such as the D+n or D-n of Smirnov, primarily, it seems, because of the difficulty of computing the distribution of Dn . As far as we know, no easy way to compute that distribution has ever been provided in the 70+ years since Kolmogorov's fundamental paper. We provide one here, a C procedure that provides Pr(Dn .999 with n's of several thousand, we provide a quick approximation that gives accuracy to the 7th digit for such cases.

  5. Identification and Expression Profiling of the Auxin Response Factors in Dendrobium officinale under Abiotic Stresses.

    Science.gov (United States)

    Chen, Zhehao; Yuan, Ye; Fu, Di; Shen, Chenjia; Yang, Yanjun

    2017-05-04

    Auxin response factor (ARF) proteins play roles in plant responses to diverse environmental stresses by binding specifically to the auxin response element in the promoters of target genes. Using our latest public Dendrobium transcriptomes, a comprehensive characterization and analysis of 14 DnARF genes were performed. Three selected DnARFs, including DnARF1, DnARF4, and DnARF6, were confirmed to be nuclear proteins according to their transient expression in epidermal cells of Nicotiana benthamiana leaves. Furthermore, the transcription activation abilities of DnARF1, DnARF4, and DnARF6 were tested in a yeast system. Our data showed that DnARF6 is a transcriptional activator in Dendrobium officinale. To uncover the basic information of DnARF gene responses to abiotic stresses, we analyzed their expression patterns under various hormones and abiotic treatments. Based on our data, several hormones and significant stress responsive DnARF genes have been identified. Since auxin and ARF genes have been identified in many plant species, our data is imperative to reveal the function of ARF mediated auxin signaling in the adaptation to the challenging Dendrobium environment.

  6. Chinese medicines in the treatment of experimental diabetic nephropathy.

    Science.gov (United States)

    Liu, Jing-Yi; Chen, Xiao-Xin; Tang, Sydney Chi-Wai; Sze, Stephen Cho-Wing; Feng, Yi-Bin; Lee, Kai-Fai; Zhang, Kalin Yan-Bo

    2016-01-01

    Diabetic nephropathy (DN) is a severe micro vascular complication accompanying diabetes mellitus that affects millions of people worldwide. End-stage renal disease occurs in nearly half of all DN patients, resulting in large medical costs and lost productivity. The course of DN progression is complicated, and effective and safe therapeutic strategies are desired. While the complex nature of DN renders medicines with a single therapeutic target less efficacious, Chinese medicine, with its holistic view targeting the whole system of the patient, has exhibited efficacy for DN management. This review aims to describe the experimental evidence for Chinese medicines in DN management, with an emphasis on the underlying mechanisms, and to discuss the combined use of herbs and drugs in DN treatment.

  7. Double network hydrogel with high mechanical strength:Performance, progress and future perspective

    Institute of Scientific and Technical Information of China (English)

    CHEN YongMei; DONG Kun; LIU ZhenQi; XU Feng

    2012-01-01

    With high water content (~90 wt%) and significantly improved mechanical strength (~MPa),double network (DN) hydrogels have emerged as promising biomaterials with widespread applications in biomedicine.In recent years,DN hydrogels with extremely high mechanical strength have achieved great advance,and scientists have designed a series of natural and biomimetic DN hydrogels with novel functions including low friction,low wear,mechanical anisotropy and cell compatibility.These advances have also led to new design of biocompatible DN hydrogels for regeneration of tissues such as cartilage.In this paper,we reviewed the strategies of designing high-strength DN hydrogel and analyzed the factors that affect DN hydrogel properties.We also discussed the challenges and future development of the DN hydrogel in view of its potential as biomaterials for their biomedical applications.

  8. Low molecular weight fucoidan modulates P-selectin and alleviates diabetic nephropathy.

    Science.gov (United States)

    Xu, Yingjie; Zhang, Quanbin; Luo, Dali; Wang, Jing; Duan, Delin

    2016-10-01

    Diabetic nephropathy (DN) is a serious microvascular complication that can lead to chronic and end-stage renal failure. It is understood that inflammation is associated with the onset and process of DN. Low molecular weight fucoidan (LMWF) isolated from Saccharina japonica has anti-inflammatory properties. Therefore, this study aimed to explore the mechanism of LMWF in DN model induced by streptozotocin. The biochemical indices levels showed LMWF reduced the DN diagnostic indices to protect renal function. The HE stained sections exhibited LMWF protected normal morphological structures and reduced inflammatory cell infiltration in the kidneys of DN rats. Furthermore, the levels of P-selectin and selectin-dependent inflammatory cytokines resulting from LMWF were obviously decreased at both the transcriptional and protein levels. Thus, our results found that LMWF protected the renal function in DN rats and alleviated inflammation through the modulation of P-selectin and inflammatory cytokines. LMWF may have therapeutic potential against DN.

  9. Dicty_cDB: Contig-U05754-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available on 599 Leaf cDNA 4... 44 3.2 1 ( DN828144 ) G.hir-45, 60 hrs post inoculation 465 L...eaf cDNA 4... 44 3.2 1 ( DN828033 ) G.hir-45, 60 hrs post inoculation 354 Leaf cDNA 4... 44 3.2 1 ( DN828029... ) G.hir-45, 60 hrs post inoculation 350 Leaf cDNA 4... 44 3.2 1 ( DN827879 ) G.hir-45, 60 hrs post inocul...ation 200 Leaf cDNA 4... 44 3.2 1 ( DN827736 ) G.hir-45, 60 hrs post inoculation 57... Leaf cDNA 45... 44 3.2 1 ( DN827594 ) G.hir-20, 30 hrs post inoculation 904 Leaf cDNA 2... 44 3.2 1 ( DN827

  10. Genètica de la variabilitat isoenzimàtica en l'avellaner: identificació varietal

    OpenAIRE

    1993-01-01

    L'estudi del polimorfisme dels isoenzims en 9 progenies d'avellaner (Corylusavellana L.), utilitzant extractes de fulla i de pol·len, ha permès establir la genèticade sis sistemes enzimàtics en aquesta espècie: aconitasa (AGO), 6-fosfogluconatdeshidrogenasa (6PGD), fosfoglucoisomerasa (PGI), fosfoglucomutasa (PGM),glutamat oxalacetat transaminasa (GOT) i malat deshidrogenasa (MDH).La variació observada s'ha pogut explicar per l'existència de 10 gens d'herènciaMendeliana: Aco-1 (4 al·lels), Ac...

  11. Estudio de la expresión de enzimas del metabolismo de aminoácidos en Lactococcus lactis

    OpenAIRE

    García-Cayuela, Tomás

    2011-01-01

    Los aminoácidos son fundamentales para la supervivencia y el desarrollo de bacterias. Son las principales fuentes de nitrógeno y están implicados en la producción de energía, el control del pH intracelular y la regeneración de cofactores. Además, son los precursores de una larga variedad de compuestos volátiles en Lactococcus lactis y, por ello, diversas enzimas son consideradas clave para su formación, como aminotransferasas, deshidrogenasas, liasas y decarboxilasas, entre otras. Estas enzim...

  12. Evolución molecular de los genes Badh

    OpenAIRE

    2004-01-01

    Se hizo un análisis comparativo entre 18 secuencias de aminoácidos de enzimas Betaina Aldehído Deshidrogenasas (BADH), que participan en la síntesis del osmoprotector glicina betaina, en algunas especies vegetales pertenecientes a familias económicamente importantes, como Chenopodiaceae, Amaranthaceae, Brassicaceae, Acanthaceae y Poaceae. El objetivo fue estudiar la evolución molecular de la vía de síntesis de la glicina betaina y de los genes que codifican para las enzimas que en ella partic...

  13. Cáncer anaplásico de tiroides. Reporte de caso.

    OpenAIRE

    Pinto-Valdivia, Miguel; Servicio de Endocrinología, Hospital Nacional Cayetano Heredia. Facultad de Medicina Alberto Hurtado, Universidad Peruana Cayetano Heredia. Lima.; Ortiz-Torres, Milagros; Servicio de Endocrinología, Hospital Nacional Cayetano Heredia. Facultad de Medicina Alberto Hurtado, Universidad Peruana Cayetano Heredia. Lima.; Villena-Chávez, Jaime; Servicio de Endocrinología, Hospital Nacional Cayetano Heredia. Facultad de Medicina Alberto Hurtado, Universidad Peruana Cayetano Heredia. Lima.; Chian-García, César; Departamento de Anatomía Patológica, Hospital Nacional Arzobispo Loayza. Lima.

    2012-01-01

    Mujer de 40 años, sin antecedente de patología tiroidea, con historia de enfermedad de cuatro semanas caracterizado por baja de peso, aumento rápido del tamaño de la glándula tiroides y dolor óseo generalizado. El examen físico mostró bocio multinodular no doloroso con múltiples ganglios cervicales. Los análisis de laboratorio mostraron anemia y niveles elevados de transaminasas, fosfatasa alcalina y deshidrogenasa láctica. El perfil tiroideo fue normal y los anticuerpos antiperoxidasa tiroid...

  14. Reporte de 5 casos con enfermedad de jarabe de arce en un periodo de 16 años. Tamiz metabólico ampliado, detección de errores innatos del metabolismo en el hospital para el niño poblano

    Directory of Open Access Journals (Sweden)

    Juan Manuel Aparicio-Rodríguez

    2013-02-01

    Full Text Available Los Errores innatos del metabolismo (EIM desde 1908 más frecuentes como fenilcetonuria, y galactosemia, así como albinismo, cistinuria y Porfiria y con una incidencia más rara Enfermedad de Orina con Olor de Jarabe de Arce (EOOJA que se reporta en este estudio. Secundario a una deficiencia del complejo alfa-cetoacido deshidrogenasa (BCKDC, dando una alteración de los aminoácidos leucina, isoleucina y valina, originando productos tóxicos en sangre y orina.

  15. Anemia hemolítica autoinmune postinfección por virus de la hepatitis A. Informe de caso; Autoimmune haemolytic anaemia associated to hepatitis A. Case report

    OpenAIRE

    Claudia Lucía Sossa Melo, MD; Sara Inés Jiménez Sanguino, MD; Carlos Andrés Pérez Martínez, MD; Amaury Alexis Amaris Vergara, MD; Luis Antonio Salazar Montaña, MD; Ángela Peña Castellanos, MD; Jesica Liliana Pinto Ramírez; Laura Andrea Rincón Arenas

    2010-01-01

    La anemia hemolítica autoinmune se asocia con una variedad de virus hepatotrópicos, en particular citomegalovirus (CMV), virus del Epstein-Barr y de la hepatitis B. No es frecuente dentro de la historia natural de la hepatitis A, la aparición de anemia hemolítica, y cuando se presenta, generalmente se asocia a deficiencia de glucosa-6-fosfato deshidrogenasa. Presentamos el caso de un paciente de sexo masculino sin hemólisis previa, con astenia e ictericia de dos meses de evolución y hepatomeg...

  16. Indicadores microbiológicos como marcadores de la contaminación y recuperación de suelos contaminados con queroseno

    Directory of Open Access Journals (Sweden)

    E. Pérez

    2008-01-01

    actividad deshidrogenasa (DH, la respiración basal (RB y la concentración residual de hidrocarburos (THC. El recuento de heterótrofos cultivables (ufcs resultó ser menos efectivo que las variables anteriores para monitorizar el proceso de biorremediación. Tras 2-4 semanas de adaptación, el incremento en la actividad de los microorganismos aumentó la tasa de degradación. A medida que los hidrocarburos disponibles se consumían, la actividad disminuyó y con ella las tasas de degradación.

  17. Distrofia muscular de Duchenne y defecto de oxidación de ácidos grasos en un paciente pediátrico

    OpenAIRE

    Alejandra Acosta-Gualandri; Mildred Jiménez-Hernández

    2014-01-01

    Paciente pediátrico, con el diagnóstico de distrofia muscular de Duchenne y deficiencia de acil-CoA deshidrogenasa de cadenas medias, ambas patologías confirmadas por medio de análisis molecular, al detectarse la deleción de los exones 45 al 50 en el gen DMD, y la mutación A985G en estado homocigoto del gen ACADM. El paciente presenta una enfermedad metabólica debido a un trastorno en la oxidación mitocondrial de los ácidos grasos, con una clínica caracterizada por hipoglicemias no cetósicas,...

  18. Mecanismos implicados en las conductas inducidas por el alcohol: el papel de los enzimas cerebrales responsables de metabolismo del acetaldehido

    OpenAIRE

    Escarabajal-Arrieta, María-Dolores

    2013-01-01

    [ES] Existe un constante aumento del consumo de bebidas alcohólicas unido a un porcentaje de personas alcohólicas cada vez mayor lo que demanda nuevos estudios que puedan aportar algo de claridad a la pregunta de por qué la gente bebe. En este sentido se presenta el estudio realizado sobre los sistemas enzimáticos implicados en el metabolismo del etanol tras las manipulaciones farmacológicas de la actividad de la catalasa y de la aldehído deshidrogenasa (ALDH) cerebrales, manipulaciones q...

  19. Reporte de 5 casos con enfermedad de jarabe de arce en un periodo de 16 años. Tamiz metabólico ampliado, detección de errores innatos del metabolismo en el hospital para el niño poblano

    OpenAIRE

    Juan Manuel Aparicio-Rodríguez

    2013-01-01

    Los Errores innatos del metabolismo (EIM) desde 1908 más frecuentes como fenilcetonuria, y galactosemia, así como albinismo, cistinuria y Porfiria y con una incidencia más rara Enfermedad de Orina con Olor de Jarabe de Arce (EOOJA) que se reporta en este estudio. Secundario a una deficiencia del complejo alfa-cetoacido deshidrogenasa (BCKDC), dando una alteración de los aminoácidos leucina, isoleucina y valina, originando productos tóxicos en sangre y orina.

  20. EVALUACION DEL DAÑO OXIDATIVO INDUCIDO POR LOS RADICALES PEROXILOS HIDROSOLUBLES SOBRE PROTEINAS DEL GLOBO OCUALR

    OpenAIRE

    ARENAS LISSI, ANDREA BEATRIZ; ARENAS LISSI; ANDREA BEATRIZ

    2013-01-01

    En esta tesis se estudiaron las modificaciones tanto químicas como estructurales de diferentes proteínas presentes en el globo ocular (lisozima, glucosa 6-fosfato deshidrogenasa y proteína de lente de bovino) causadas por la acción de los radicales peroxilos generados a partir de la termólisis del cloro hidrato de 2,2'azobis(amidinopropano) en presencia de oxígeno. El estudio de los geles de poliacrilamida, dieron cuenta de la ocurrencia de entrecruzamiento y fraccionamiento proteico...

  1. Xantinuria: una causa rara de urolitiasis en el gato

    OpenAIRE

    2012-01-01

    La xantinuria es una patología que se presenta raramente en los gatos. Su etiología puede tener origen genético o deberse a una inhibición yatrogénica de la enzima xantina deshidrogenasa, que generalmente se manifiesta con urolitiasis. En este trabajo se informa el hallazgo de dos urolitos de xantina en dos gatos, un macho y una hembra, de raza Europea de pelo corto, no emparentados. Los urolitos fueron analizados mediante microscopía estereoscópica, espectroscopía infrarroja y microscopía el...

  2. Producción de xilitol a partir de levaduras nativas colombianas*

    OpenAIRE

    Vanegas Córdoba, Isleny Andrea; Yepes Pérez, María Del Socorro; Ruiz Villadiego, Orlando Simón

    2007-01-01

    * Este artículo corresponde a la presentación oral que obtuvo el tercer lugar en la sala de bioprocesos, bioprospección y ambiental del segundo Congreso Colombiano de Biotecnología. El xilitol es un edulcorante alternativo con características similares a la sacarosa que ha despertado gran interés debido principalmente a su uso seguro en pacientes diabéticos y aquellos deficientes en glucosa-6-fosfato-deshidrogenasa. Su síntesis química es costosa y genera subproductos indeseables, mientra...

  3. ANALISIS FUNCIONAL DE LOS GENES QUE CODIFICAN PARA LAS SUBUNIDADES DEL COMPLEJO II MITOCONDRIAL EN ARABIDOPSIS THALIANA

    OpenAIRE

    2006-01-01

    En todos los organismos la biogénesis mitocondrial depende de la expresión de los genomas mitocondrial y nuclear. No obstante, se sabe poco acerca de la estructura, la regulación y la importancia fisiológica de los genes nucleares que codifican para proteínas mitocondriales, particularmente en plantas. Esto, a pesar que la mayor parte de las proteínas mitocondriales está codificada por genes nucleares. El complejo II mitocondrial (succinato deshidrogenasa) cataliza la oxidación de succi...

  4. Primer caso de hemoglobina Köln (codon98 GTG>ATG en Costa Rica

    Directory of Open Access Journals (Sweden)

    Walter Cartín

    2010-03-01

    Full Text Available La exposición a ciertos medicamentos puede provocar anemia hemolítica con presencia de cuerpos de Heinz en sangre periférica. Esta anemia puede presentarse por sobredosis de medicamentos, tanto en individuos sanos como en personas con deficiencias enzimáticas como la glucosa-6-fosfato deshidrogenasa, o en presencia de hemoglobinas inestables. Este reporte muestra un caso de anemia hemolítica con cuerpos de Heinz, debido a la presencia de una hemoglobina inestable, cuyos estudios moleculares y HPLC confirmaron el primer caso descrito de hemoglobina Köln (Val98Met en Costa Rica.

  5. Anemia hemolítica autoinmune postinfección por virus de la hepatitis A. Informe de caso; Autoimmune haemolytic anaemia associated to hepatitis A. Case report

    OpenAIRE

    Claudia Lucía Sossa Melo, MD; Sara Inés Jiménez Sanguino, MD; Carlos Andrés Pérez Martínez, MD; Amaury Alexis Amaris Vergara, MD; Luis Antonio Salazar Montaña, MD; Ángela Peña Castellanos, MD; Jesica Liliana Pinto Ramírez; Laura Andrea Rincón Arenas

    2010-01-01

    La anemia hemolítica autoinmune se asocia con una variedad de virus hepatotrópicos, en particular citomegalovirus (CMV), virus del Epstein-Barr y de la hepatitis B. No es frecuente dentro de la historia natural de la hepatitis A, la aparición de anemia hemolítica, y cuando se presenta, generalmente se asocia a deficiencia de glucosa-6-fosfato deshidrogenasa. Presentamos el caso de un paciente de sexo masculino sin hemólisis previa, con astenia e ictericia de dos meses de evolución y hepatomeg...

  6. Conciderações sobre a oxidação lipíca e seus limitações: o possível papel do fracionamento de carboidratos

    Directory of Open Access Journals (Sweden)

    André de Camargo Smolarek

    2012-05-01

    Full Text Available Os ácidos graxos estocados principalmente na forma de triacilglicerois no tecido adiposo ou em reduzidas quantidades no tecido muscular representam uma fundamental via energética em exercícios de intensidade leve a moderada. Caracterizam-se pela alta disponibilidade de trifosfato de adenosina (ATP possuindo relevância em termos quantitativos nos exercícios de longa duração (aeróbios. De forma simplificada, a oxidação lipídica divide-se em mobilização e transporte dos ácidos graxos, β-oxidação e formação de acetil-CoA para então ser metabolizado no ciclo de Krebs. Nesse trabalho levantaremos a hipótese de que a contínua metabolização dos ácidos graxos depende em parte do fracionamento dos carboidratos (CHO. Após a glicólise, com a formação de piruvato, há formação de oxaloacetato através da enzima piruvato carboxilase. O ciclo de Krebs inicia-se com a condensação de acetil-CoA e oxaloacetato. Quando há restrição nutricional ou período extenso de atividade física (AF, há um decréscimo na quantidade de glicogênio gerando uma queda na formação de piruvato. A oxidação dos AG no ciclo de Krebs depende da acessibilidade adequada de oxaloacetato para condensar-se com o acetil-CoA. Uma queda na quantidade de CHO incitará uma diminuição conseqüente na produção de oxaloacetato o que resultará em diminuição oxidativa dos ácidos graxos. Sendo assim, o objetivo do presente estudo foi verificar, através da revisão bibliográfica, considerações sobre a oxidação lipídica e suas limitações visando o possível papel do fracionamento dos CHO.

  7. Releasing dentate nucleus cells from Purkinje cell inhibition generates output from the cerebrocerebellum.

    Directory of Open Access Journals (Sweden)

    Takahiro Ishikawa

    Full Text Available The cerebellum generates its vast amount of output to the cerebral cortex through the dentate nucleus (DN that is essential for precise limb movements in primates. Nuclear cells in DN generate burst activity prior to limb movement, and inactivation of DN results in cerebellar ataxia. The question is how DN cells become active under intensive inhibitory drive from Purkinje cells (PCs. There are two excitatory inputs to DN, mossy fiber and climbing fiber collaterals, but neither of them appears to have sufficient strength for generation of burst activity in DN. Therefore, we can assume two possible mechanisms: post-inhibitory rebound excitation and disinhibition. If rebound excitation works, phasic excitation of PCs and a concomitant inhibition of DN cells should precede the excitation of DN cells. On the other hand, if disinhibition plays a primary role, phasic suppression of PCs and activation of DN cells should be observed at the same timing. To examine these two hypotheses, we compared the activity patterns of PCs in the cerebrocerebellum and DN cells during step-tracking wrist movements in three Japanese monkeys. As a result, we found that the majority of wrist-movement-related PCs were suppressed prior to movement onset and the majority of wrist-movement-related DN cells showed concurrent burst activity without prior suppression. In a minority of PCs and DN cells, movement-related increases and decreases in activity, respectively, developed later. These activity patterns suggest that the initial burst activity in DN cells is generated by reduced inhibition from PCs, i.e., by disinhibition. Our results indicate that suppression of PCs, which has been considered secondary to facilitation, plays the primary role in generating outputs from DN. Our findings provide a new perspective on the mechanisms used by PCs to influence limb motor control and on the plastic changes that underlie motor learning in the cerebrocerebellum.

  8. Clinical Characteristics of Patients with Diabetic Nephropathy on Maintenance Hemodialysis: A Multicenter Cross-sectional Survey in Anhui Province, Eastern China

    Institute of Scientific and Technical Information of China (English)

    Hu Chen; De-Guang Wang; Liang Yuan; Gui-Ling Liu; Heng-Jie He; Juan Wang; Sen Zhang

    2016-01-01

    Background:The incidence of diabetic nephropathy (DN) increases year by year.However,clinical characteristics of DN patients on maintenance hemodialysis (MHD) were rarely reported in China.The purpose of this study was to examine the clinical characteristics of the DN patients on MHD in Anhui Province,Eastern China.Methods:The clinical data of MHD patients in the hemodialysis centers of 26 hospitals in Anhui Province from January 1,2014,to March 31,2014,were examined.The differences between DN patients and non-DN patients were compared regarding vascular access,nutritional status,mineral and bone disorder,and other indexes.Results:Among the selected 2768 adult MHD patients,427 had DN.The incidence of hypertension,coronary heart disease,and cerebral thrombus in DN patients was 94.1%,21.5%,and 15.0%,respectively,which were higher than those in non-DN patients (P < 0.001).Category of vascular access for hemodialysis in DN patients was arteriovenous fistula (AVF) (87.4% [373/427]) and tunneled cuffed catheter (TCC) (11.2% [48/427]).The percentage of AVF was significantly lower than that ofnon-DN patients (P < 0.001),and percentage of TCC was significantly higher than that of non-DN patients (P < 0.001).Hemoglobin achievement rate in DN patients was 32.0%.The incidence of hypoalbuminemia was 24.7%,significantly higher than that in non-DN patients (P < 0.001).The achievement rate of the target range in mineral values was 55.9% in corrected serum calcium level,30.1% in serum phosphorus level,and 49.3% in intact parathyroid hormone (iPTH) level in DN patients.Compared with non-DN patients,the achievement rate of serum phosphorus was significantly higher in DN patients.Conclusions:DN patients on MHD in Anhui province exhibited different clinical characteristics compared to non-DN hemodialysis patients.They presented higher percentage in TCC use and cardiovascular complication,lower serum albumin and iPTH levels than those in non-DN patients.

  9. Dry needling versus acupuncture: the ongoing debate.

    Science.gov (United States)

    Zhou, Kehua; Ma, Yan; Brogan, Michael S

    2015-12-01

    Although Western medical acupuncture (WMA) is commonly practised in the UK, a particular approach called dry needling (DN) is becoming increasingly popular in other countries. The legitimacy of the use of DN by conventional non-physician healthcare professionals is questioned by acupuncturists. This article describes the ongoing debate over the practice of DN between physical therapists and acupuncturists, with a particular emphasis on the USA. DN and acupuncture share many similarities but may differ in certain aspects. Currently, little information is available from the literature regarding the relationship between the two needling techniques. Through reviewing their origins, theory, and practice, we found that DN and acupuncture overlap in terms of needling technique with solid filiform needles as well as some fundamental theories. Both WMA and DN are based on modern biomedical understandings of the human body, although DN arguably represents only one subcategory of WMA. The increasing volume of research into needling therapy explains its growing popularity in the musculoskeletal field including sports medicine. To resolve the debate over DN practice, we call for the establishment of a regulatory body to accredit DN courses and a formal, comprehensive educational component and training for healthcare professionals who are not physicians or acupuncturists. Because of the close relationship between DN and acupuncture, collaboration rather than dispute between acupuncturists and other healthcare professionals should be encouraged with respect to education, research, and practice for the benefit of patients with musculoskeletal conditions who require needling therapy.

  10. Polymorphism of angiotensin-converting enzyme (rs4340 and diabetic nephropathy in Caucasians with type 2 diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Šeruga M

    2016-12-01

    Full Text Available Diabetic nephropathy (DN is the leading cause of endstage renal disease (ESRD in developed countries. Several environmental and genetic factors predict the development and progression of DN. The renin-angiotensin system was demonstrated to be involved in the development of DN. We evaluated the association between rs4340 of the angiotensin-converting enzyme (ACE gene and DN in Caucasians with type 2 diabetes mellitus (T2DM in 276 Slovenian patients with T2DM who had DN, and 375 patients without clinical signs of DN. Genetic analysis was performed with either standard polymerase chain reaction (PCR (for rs4340. Results were analyzed using the χ2 test and multivariate logistic regression analyses. We found no association between rs4340 and DN. Cystatin C was significantly higher in the DN+ group (p <0.001 than in the DN group. Cystatin C was a better marker for the estimation of renal function than estimated glomerular filtration rate (eGFR according to the modification diet in renal disease (MDRD equation mL/ min. We concluded that there was no association between the rs4340 of the ACE gene and DN in Caucasian patients who have T2DM.

  11. Bone-marrow mesenchymal stem cell transplantation to treat diabetic nephropathy in tree shrews.

    Science.gov (United States)

    Pan, Xing-Hua; Yang, Xiao-Yan; Yao, Xiang; Sun, Xiao-Mei; Zhu, Lu; Wang, Jin-Xiang; Pang, Rong-Qing; Cai, Xue-Min; Dai, Jie-Jie; Ruan, Guang-Ping

    2014-07-01

    Diabetic nephropathy (DN) is a common microvascular complication of diabetes. We used a new DN model in tree shrews to validate the use of bone-marrow mesenchymal stem cell (BM-MSC) transplantation to treat DN. The DN tree shrew model was established by a high-sugar and high-fat diet and four injections of streptozotocin. 4',6-Diamidino-2-phenylindole labelled BM-MSCs were injected into tree shrews. The DN tree shrew model was successfully established. Blood glucose was significantly increased ( p < 0.01) during the entire experiment. DN tree shrews showed dyslipidemia, insulin resistance and increased 24-h proteinuria. At 21 days after BM-MSC transplantation, glucose and levels of triglycerides, total cholesterol and 24-h urine volume were lower than in tree shrews with DN alone ( p < 0.01) but were still higher than control values ( p < 0.01). Levels of creatinine and urea nitrogen as well as 24-h proteinuria were lower for DN tree shrews with BM-MSCs transplantation than DN alone ( p < 0.05). High-sugar and high-fat diet combined with STZ injection can induce a tree shrew model of DN. BM-MSCs injection can home to damaged kidneys and pancreas, for reduced 24-h proteinuria and improved insulin resistance.

  12. A retrospective study on the impact of comorbid depression or anxiety on healthcare resource use and costs among diabetic neuropathy patients

    Directory of Open Access Journals (Sweden)

    Bao Yanjun

    2009-06-01

    Full Text Available Abstract Background Diabetic neuropathy (DN is a common complication of diabetes that has significant economic burden, especially for patients with comorbid depression or anxiety. This study examines and quantifies factors associated with healthcare costs among patients diagnosed with diabetic neuropathy (DN with or without a comorbid diagnosis of depression or anxiety (DA using retrospective administrative claims data. No study has examined the differences in economic outcomes depending on the presence of comorbid DA disorders. Methods Over-age-18 individuals with 1+ diagnosis of DN in 2005 were selected. The first observed DN claim was considered the "index date." All individuals had a 12-month pre-index and follow-up period. For both under-age-65 commercially insured and over-age-65 individuals with employer-sponsored Medicare supplemental insurance, we constructed 2 subgroups for individuals with DA (DN-DA or without (DN-only. Patients' clinical characteristics over pre-index period were compared. Multivariate regressions were performed to assess whether DN-DA patients had higher utilization of healthcare resources and costs than DN-only patients, controlling for demographic and clinical characteristics. Results We identified 16,831 DN-only and 1,699 DN-DA patients in the Medicare supplemental cohort, as well as 17,205 and 3,105 in the commercially insured. DN-DA patients had higher prevalence of diabetes-related comorbidities for cardiovascular disease, cerebrovascular/peripheral vascular disease, nephropathy, obesity, and hypoglycemic events than DN-only patients (all p Conclusion These findings indicate that the healthcare costs were significantly higher for DN patients with depression or anxiety relative to those without such comorbid disorders.

  13. Efeitos de estatinas sobre a cetonemia, glicemia e lipidemia

    OpenAIRE

    2008-01-01

    As estatinas deprimem a colesterologênese elevando os níveis de acetil-CoA, o que, segundo a lei de ação das massas, poderia exacerbar a cetogênese e inibir a piruvato desidrogenase, com diminuição da glicólise. O objetivo desse trabalho foi investigar os efeitos do uso de estatinas sobre a cetonemia e a glicemia em pacientes normoglicêmicos. A população estudada foi constituída de 9 pacientes que receberam prescrição para utilização de estatina (40 mg/dia de sinvastatina ou 10...

  14. Obtención de ácido láctico. Fermentación en fase dual mediante Actinobacilus Succinogenes

    OpenAIRE

    Ayala Varela, Flavio Celín

    2015-01-01

    Se presentan los resultados obtenidos para la obtención de ácido láctico partiendo de glucosa como sustrato, utilizando el microorganismo Actinobacillus succinogenes, favoreciendo la ruta menos común hacia el piruvato con las condiciones adecuadas: Medio de crecimiento; 53286 Brain Heart Broth (SIGMA-ALDRICH), medio de fermentación; 60 g glucosa, 30 g extracto de levadura, 2 g urea, 2 g MgCl2·6H2O, 1.99 g CaCl2. 2H2O, 0.11 g MnCl2. 4H2O, 4.4 g Na2HPO4, 4,06 g NaH2PO4. 2H2O (composición por li...

  15. Identificação de Mycobacterium bovis em cepas micobacterianas isoladas espécimes clínicos humanos em um complexo hospitalar na cidade do Rio de Janeiro Identification of Mycobacterium bovis among mycobacterial isolates from human clinical specimens at a university hospital in Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Luciana Fonseca Sobral

    2011-10-01

    Full Text Available Entre 2005 e 2006, 8.121 espécimes clínicos enviados ao Laboratório de Micobactérias do Hospital Universitário Clementino Fraga Filho/Instituto de Doenças do Tórax, no Rio de Janeiro, RJ, foram inoculados em meio Löwenstein-Jensen contendo glicerol e piruvato. Desses espécimes, 79 isolados de micobactérias tiveram crescimento somente em meio com piruvato, sendo selecionados para a identificação presuntiva de Mycobacterium bovis. Esses isolados foram submetidos à identificação por testes bioquímicos, amplificação por PCR com primers específicos (Rv0577 e Rv1510 e teste de suscetibilidade à pirazinamida. Todas as cepas apresentaram padrões fenotípicos e genotípicos de M. tuberculosis, não sendo detectado M. bovis.In 2005 and 2006, 8,121 clinical specimens submitted to the Mycobacteriology Laboratory of the Clementino Fraga Filho University Hospital/Thoracic Diseases Institute, in the city of Rio de Janeiro, Brazil, were inoculated on Löwenstein-Jensen medium containing glycerol and pyruvate. There were 79 mycobacteria isolates that presented growth only on pyruvate-containing medium, and those isolates were selected for the presumptive identification of Mycobacterium bovis. The selected isolates were screened with biochemical tests, PCR amplification (with the specific primers Rv0577 and Rv1510, and pyrazinamide susceptibility tests. All of the strains isolated showed specific phenotypical and genotypical patterns characteristic of M. tuberculosis, and no M. bovis strains were detected.

  16. Alteraciones bioquímicas de los lípidos en los alimentos vegetales. II. Metabolismo de los hidroperóxidos lipídicos

    Directory of Open Access Journals (Sweden)

    Zamora, R.

    1991-06-01

    Full Text Available The enzymes of the lipoxygenase pathway in plant foods are reviewed. This part analyzes the enzymes related to the descomposition or transformation of the lipidic hydroperoxides. Lipoxygenase, hydroperoxide lyase, enal isomerase, alcohol dehydrogenase, aldehyde dehydrogenase, hydroperoxide dehydrase, alene oxide ciclase and 12-Oxo-phytodienoic acid reductase in plants are described. Posible control mechanisms of the lipoxygenase pathway are also considered.

    Los enzimas responsables de la degradación enzimática de los lípidos en los alimentos vegetales son examinados en este trabajo. Esta parte describe la descomposición o transformación de los hidroperóxidos lipídicos producida por la lipoxigenasa, hidroperóxido liasa, enal isomerasa, alcohol deshidrogenasa, aldehído deshidrogenasa, hidroperóxido dehidrasa, óxido de aleño ciclasa y ácido 12-Oxofitodienoico reductasa, así como los distintos mecanismos de control de la ruta.

  17. Dicty_cDB: Contig-U13210-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available DR451089 ) WS00954.BR_K12 IS-B-N-A-10 Picea engelmannii x Pi... 46 0.35 1 ( DN203564 ) USDA-FP_139589 5th Instar Glass...y-winged Sharpshoo... 46 0.35 1 ( DN203476 ) USDA-FP_139501 5th Instar Glass...y-winged Sharpshoo... 46 0.35 1 ( DN203471 ) USDA-FP_139496 5th Instar Glassy-winged Sharpshoo... 46 0....35 1 ( DN203427 ) USDA-FP_139452 5th Instar Glassy-winged Sharpshoo... 46 0.35 1 ( DN203388 ) USDA-FP_139413 5th Instar Glass...y-winged Sharpshoo... 46 0.35 1 ( DN199204 ) USDA-FP_135918 5th Instar Glass

  18. Reliable Designing of Stand-alone PV/FC Hybrid System

    Directory of Open Access Journals (Sweden)

    Saber Arabi Nowdeh

    2013-06-01

    Full Text Available Application of renewable energy sources has shown a perfect potential as a form of contribution to conventional power generation systems. This paper presents a hybrid system based on photovoltaic (PV module and PEM (proton exchange membrane fuel cell (FC with the aim of selling electricity to distribution network (DN and improving its reliability. In this paper, moreover supplying the load electricity of system, the proposed hybrid system is capable to sale electricity to DN and by electricity injection causes DN reliability improvement. The revenue from selling electricity to DN is considered as the system profit (SP. An optimization is applied to maximize the SP using GAMS environment. This study claims that moreover load electricity provision, electricity can also be sold to DN by proposed hybrid system and reliability of DN in load supplement can be increased by injecting the electricity to it.

  19. Advances in Murine Models of Diabetic Nephropathy

    Science.gov (United States)

    Kong, Li-li; Wu, Hao; Cui, Wen-peng; Zhou, Wen-hua; Luo, Ping; Sun, Jing; Yuan, Hang; Miao, Li-ning

    2013-01-01

    Diabetic nephropathy (DN) is one of the microvascular complications of both type 1 and type 2 diabetes, which is also associated with a poor life expectancy of diabetic patients. However, the pathogenesis of DN is still unclear. Thus, it is of great use to establish appropriate animal models of DN for doing research on pathogenesis and developing novel therapeutic strategies. Although a large number of murine models of DN including artificially induced, spontaneous, and genetically engineered (knockout and transgenic) animal models have been developed, none of them develops renal changes sufficiently reflecting those seen in humans. Here we review the identified murine models of DN from the aspects of genetic background, type of diabetes, method of induction, gene deficiency, animal age and gender, kidney histopathology, and phenotypic alterations in the hope of enhancing our comprehension of genetic susceptibility and molecular mechanisms responsible for this disease and providing new clues as to how to choose appropriate animal models of DN. PMID:23844375

  20. Microbubbles and Ultrasound: Therapeutic Applications in Diabetic Nephropathy.

    Science.gov (United States)

    Cao, Wei J; Matkar, Pratiek N; Chen, Hao H; Mofid, Azadeh; Leong-Poi, Howard

    2016-01-01

    Diabetic nephropathy (DN) remains one of the most common causes of end-stage renal disease. Current therapeutic strategies aiming at optimization of serum glucose and blood pressure are beneficial in early stage DN, but are unable to fully prevent disease progression. With the limitations of current medical therapies and the shortage of available donor organs for kidney transplantation, the need for novel therapies to address DN complications and prevent progression towards end-stage renal failure is crucial. The development of ultrasound technology for non-invasive and targeted in-vivo gene delivery using high power ultrasound and carrier microbubbles offers great therapeutic potential for the prevention and treatment of DN. The promising results from preclinical studies of ultrasound-mediated gene delivery (UMGD) in several DN animal models suggest that UMGD offers a unique, non-invasive platform for gene- and cell-based therapies targeted against DN with strong clinical translation potential.

  1. Pre-Concentration for Improved Long-Term Monitoring of Contaminants in Groundwater

    Science.gov (United States)

    2009-01-01

    stationary phase was an 250 mm Altech Alltima C18 (5μm) analytical column with an isocratic 50:50 methanol:water mobile phase. A 20 μL sample...0.0 0.5 1.0 1.5 T ar ge t C on ce nt ra tio n (p pm ) 0 2 4 6 52 54 HM X RD X TN B tet ryl NB TN T 2-A DN T 4-A DN T 2,4 -D NT 2-N T 4-N T 3-N T DN B...HM X RD X TN B tet ryl NB TN T 2-A DN T 4-A DN T 2,4 -D NT 2-N T 4-N T 3-N T DN B   Figure 35. Concentration

  2. Dental enamel structure is altered by expression of dominant negative RhoA in ameloblasts.

    Science.gov (United States)

    Li, Yong; Pugach, Megan K; Kuehl, Melissa A; Peng, Li; Bouchard, Jessica; Hwang, Soon Y; Gibson, Carolyn W

    2011-01-01

    Using in vitrotooth germ cultures and analysis by confocal microscopy, ameloblasts treated with sodium fluoride were found to have elevated amounts of filamentous actin. Because this response is reduced by inhibitors of the Rho/ROCK signaling pathway, we generated mice that express dominant negative RhoA (RhoA(DN)) in ameloblasts for in vivo analysis. Expression of the EGFP-RhoA(DN) fusion protein was evaluated by RT-PCR and immunohistochemistry, and teeth were analyzed by scanning electron microscopy. The 3 strains expressed at either low (TgEGFP-RhoA(DN)-8), intermediate (TgEGFP-RhoA(DN)-2), or high (TgEGFP-RhoA(DN)-13) levels, and the molar teeth from the 3 strains had enamel hypoplasia and surface defects. We conclude that RhoA(DN) expressed in ameloblasts interferes with normal enamel development through the pathway that is induced by sodium fluoride. Copyright © 2011 S. Karger AG, Basel.

  3. Delayed neutrons measurement at the MEGAPIE target

    Energy Technology Data Exchange (ETDEWEB)

    Panebianco, S.; Bokov, P.; Dore, D.; Letourneau, A.; Prevost, A.; Ridikas, D. [CEA Saclay, Dept. d' Astrophysique de Physique des Particules, de Physique Nucleaire et de l' Instrumentation Associee (DSM/DAPNIA/SPhN), 91- Gif sur Yvette (France); Ledoux, X. [CEA Bruyeres-le-Chatel (CEA DIF, DPTA/SPN), 91 (France). Dept. de Physique Theorique et Appliquee

    2008-07-01

    In the framework of the Neutronic and Nuclear Assessment Task Group of the MEGAPIE experiment we measured the delayed neutron (DN) flux at the top of the target. The measurement was proposed mainly for radioprotection purposes since the DN flux at the top of the target has been estimated to be of the same order of magnitude as the prompt neutron flux. Given the strong model-dependence of DN predictions, the measurement of DN contribution to the total neutron activity at the top of the target was thus desired. Moreover, this measurement is complementary to the DN experiments performed at PNPI (Gatchina) on solid lead and bismuth targets. The DN measurement at MEGAPIE was performed during the start-up phase of the target. In this paper we present a detailed description of the experimental setup and some preliminary results on decay spectra. (authors)

  4. Delayed neutrons measurement at the MEGAPIE target

    CERN Document Server

    Panebianco, Stefano; Dore, Diane; Ledoux, Xavier; Letourneau, Alain; Prevost, Aurelien; Ridikas, Danas

    2007-01-01

    In the framework of the Neutronic and Nuclear Assessment Task Group of the MEGAPIE experiment we measured the delayed neutron (DN) flux at the top of the target. The measurement was proposed mainly for radioprotection purposes since the DN flux at the top of the target has been estimated to be of the same order of magnitude as the prompt neutron flux. Given the strong model-dependence of DN predictions, the measurement of DN contribution to the total neutron activity at the top of the target was thus desired. Moreover, this measurement is complementary to the DN experiments performed at PNPI (Gatchina) on solid lead and bismuth targets. The DN measurement at MEGAPIE was performed during the start-up phase of the target. In this paper we present a detailed description of the experimental setup and some preliminary results on decay spectra.

  5. Diabetic nephropathy and antioxidants.

    Science.gov (United States)

    Tavafi, Majid

    2013-01-01

    Oxidative stress has crucial role in pathogenesis of diabetic nephropathy (DN). Despite satisfactory results from antioxidant therapy in rodent, antioxidant therapy showed conflicting results in combat with DN in diabetic patients. Directory of Open Access Journals (DOAJ), Google Scholar,Pubmed (NLM), LISTA (EBSCO) and Web of Science have been searched. Treatment of DN in human are insufficient with rennin angiotensin system (RAS) blockers, so additional agent ought to combine with this management. Meanwhile based on DN pathogenesis and evidences in experimental and human researches, the antioxidants are the best candidate. New multi-property antioxidants may be improved human DN that show high power antioxidant capacity, long half-life time, high permeability to mitochondrion, improve body antioxidants enzymes activity and anti-inflammatory effects. Based on this review and our studies on diabetic rats, rosmarinic acid a multi-property antioxidant may be useful in DN patients, but of course, needs to be proven in clinical trials studies.

  6. Advances in Murine Models of Diabetic Nephropathy

    Directory of Open Access Journals (Sweden)

    Li-li Kong

    2013-01-01

    Full Text Available Diabetic nephropathy (DN is one of the microvascular complications of both type 1 and type 2 diabetes, which is also associated with a poor life expectancy of diabetic patients. However, the pathogenesis of DN is still unclear. Thus, it is of great use to establish appropriate animal models of DN for doing research on pathogenesis and developing novel therapeutic strategies. Although a large number of murine models of DN including artificially induced, spontaneous, and genetically engineered (knockout and transgenic animal models have been developed, none of them develops renal changes sufficiently reflecting those seen in humans. Here we review the identified murine models of DN from the aspects of genetic background, type of diabetes, method of induction, gene deficiency, animal age and gender, kidney histopathology, and phenotypic alterations in the hope of enhancing our comprehension of genetic susceptibility and molecular mechanisms responsible for this disease and providing new clues as to how to choose appropriate animal models of DN.

  7. Piwil 2 gene transfection changes the autophagy status in a rat model of diabetic nephropathy.

    Science.gov (United States)

    Wu, Weihua; Zhang, Maoping; Liu, Qi; Xue, Ling; Li, Ying; Ou, Santao

    2015-01-01

    This study aims to investigate effects of Piwil2 on autohpagy in a DN rat model. Sixty health SD rats were selected and divided into four group, including normal group, control, DN and Piwil2 therapy group. DN model (DN group) was established by injecting the streptozotocin (50 mg/kg) into rats. Piwil2 therapy group was injected with viral plasmid carrying Piwil2 mRNA to DN rats. The urinary protein concentrations were determined by placing the animals in individual metabolic cages for a timed urine collection every 8 weeks. Blood and soleus muscle samples were collected after animals were sacrificed. Blood glucose was examined by using commercial detection kits. Western blot assay was employed to examine expression of Beclin 1 and LC3 (LC3 I and LC3 II) protein. Results indicated that urinary protein levels were remarkably higher in DN group compared to Normal and Control group (Pdiabetic nephropathy rats.

  8. Improved elucidation of biological processes linked to diabetic nephropathy by single probe-based microarray data analysis.

    OpenAIRE

    2008-01-01

    BACKGROUND: Diabetic nephropathy (DN) is a complex and chronic metabolic disease that evolves into a progressive fibrosing renal disorder. Effective transcriptomic profiling of slowly evolving disease processes such as DN can be problematic. The changes that occur are often subtle and can escape detection by conventional oligonucleotide DNA array analyses. METHODOLOGY/PRINCIPAL FINDINGS: We examined microdissected human renal tissue with or without DN using Affymetrix oligonucleotide microarr...

  9. Dicty_cDB: Contig-U06117-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available -01-1... 36 1.1 3 ( DN796272 ) Cm_mx0_60h11_SP6 Green Shore Crab Multiple Tissue...... 34 1.7 2 ( DN796154 ) Cm_mx0_59d11_SP6 Green Shore Crab Multiple Tissue... 34 1.7 2 ( DN738904 ) Cm_mx0_50h02_SP6 Green Shore

  10. Experimental evidence for aerobic bio-denitrification

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Nitrate pollution of groundwater is paid more and more attention for its hazardous to environments and human health. A strain of DN11 was isolated from soil and used in the laboratory columns filled with various media for nitrate removal. The experimental results showed that DN11could reduce nitrate at different rates in different media under the aerobic condition. The mechanism for nitrate removal with DN11 is explained meanwhile.

  11. Gellan gum microgel-reinforced cell-laden gelatin hydrogels

    OpenAIRE

    Shin, Hyeongho; Olsen, Bradley D.; Khademhosseini, Ali

    2013-01-01

    The relatively weak mechanical properties of hydrogels remain a major drawback for their application as load-bearing tissue scaffolds. Previously, we developed cell-laden double-network (DN) hydrogels that were composed of photocrosslinkable gellan gum (GG) and gelatin. Further research into the materials as tissue scaffolds determined that the strength of the DN hydrogels decreased when they were prepared at cell-compatible conditions, and the encapsulated cells in the DN hydrogels did not f...

  12. The Protective Effect of Beraprost Sodium on Diabetic Nephropathy by Inhibiting Inflammation and p38 MAPK Signaling Pathway in High-Fat Diet/Streptozotocin-Induced Diabetic Rats

    OpenAIRE

    Li Peng; Jie Li; Yixing Xu; Yangtian Wang; Hong Du; Jiaqing Shao; Zhimin Liu

    2016-01-01

    Background. p38 mitogen-activated protein kinase (MAPK) plays a crucial role in regulating signaling pathways implicated in inflammatory processes leading to diabetic nephropathy (DN). This study aimed to examine p38 MAPK activation in DN and determine whether beraprost sodium (BPS) ameliorates DN by inhibiting inflammation and p38 MAPK signaling pathway in diabetic rats. Methods. Forty male Sprague Dawley (SD) rats were randomly divided into the normal control group, type 2 diabetic group, a...

  13. Role of Endothelial Nitric Oxide Synthase in Diabetic Nephropathy: Lessons from Diabetic eNOS Knockout Mice

    OpenAIRE

    Takamune Takahashi; Harris, Raymond C.

    2014-01-01

    Diabetic nephropathy (DN) is the leading cause of end-stage renal disease in many countries. The animal models that recapitulate human DN undoubtedly facilitate our understanding of this disease and promote the development of new diagnostic markers and therapeutic interventions. Based on the clinical evidence showing the association of eNOS dysfunction with advanced DN, we and others have created diabetic mice that lack eNOS expression and shown that eNOS-deficient diabetic mice exhibit advan...

  14. Role of Endothelial Nitric Oxide Synthase in Diabetic Nephropathy: Lessons from Diabetic eNOS Knockout Mice

    Directory of Open Access Journals (Sweden)

    Takamune Takahashi

    2014-01-01

    Full Text Available Diabetic nephropathy (DN is the leading cause of end-stage renal disease in many countries. The animal models that recapitulate human DN undoubtedly facilitate our understanding of this disease and promote the development of new diagnostic markers and therapeutic interventions. Based on the clinical evidence showing the association of eNOS dysfunction with advanced DN, we and others have created diabetic mice that lack eNOS expression and shown that eNOS-deficient diabetic mice exhibit advanced nephropathic changes with distinct features of progressive DN, including pronounced albuminuria, nodular glomerulosclerosis, mesangiolysis, and arteriolar hyalinosis. These studies clearly defined a critical role of eNOS in DN and developed a robust animal model of this disease, which enables us to study the pathogenic mechanisms of progressive DN. Further, recent studies with this animal model have explored the novel mechanisms by which eNOS deficiency causes advanced DN and provided many new insights into the pathogenesis of DN. Therefore, here we summarize the findings obtained with this animal model and discuss the roles of eNOS in DN, unresolved issues, and future investigations of this animal model study.

  15. New Experimental Models of Diabetic Nephropathy in Mice Models of Type 2 Diabetes: Efforts to Replicate Human Nephropathy

    Directory of Open Access Journals (Sweden)

    María José Soler

    2012-01-01

    Full Text Available Diabetic nephropathy (DN is the leading cause of end-stage renal disease. The use of experimental models of DN has provided valuable information regarding many aspects of DN, including pathophysiology, progression, implicated genes, and new therapeutic strategies. A large number of mouse models of diabetes have been identified and their kidney disease was characterized to various degrees. Most experimental models of type 2 DN are helpful in studying early stages of DN, but these models have not been able to reproduce the characteristic features of more advanced DN in humans such as nodules in the glomerular tuft or glomerulosclerosis. The generation of new experimental models of DN created by crossing, knockdown, or knockin of genes continues to provide improved tools for studying DN. These models provide an opportunity to search for new mechanisms involving the development of DN, but their shortcomings should be recognized as well. Moreover, it is important to recognize that the genetic background has a substantial effect on the susceptibility to diabetes and kidney disease development in the various models of diabetes.

  16. Unigene BLAST: CBRC-RNOR-08-0285 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-08-0285 gnl|UG|Rn#S14181616 UI-R-DN1-cmt-f-09-0-UI.s1 UI-R-DN1 Rattus nor...vegicus cDNA clone UI-R-DN1-cmt-f-09-0-UI 3', mRNA sequence /clone=UI-R-DN1-cmt-f-09-0-UI /clone_end=3' /gb=BQ203155 /gi=20419620 /ti=154455607 /ug=Rn.213357 /len=803 0.024 30% ...

  17. Subcellular distribution and uptake mechanism of di-n-butyl phthalate in roots of pumpkin (Cucurbita moschata) seedlings.

    Science.gov (United States)

    Lin, Qingqi; Yang, Xiuhong; Huang, Xiongfei; Wang, Shizhong; Chao, Yuanqing; Qiu, Rongliang

    2016-01-01

    Phthalate acid esters (PAEs) are of particular concern due to their potential environmental risk to human and nonhuman organisms. Although uptake of PAEs by plants has been reported by several researchers, information about the intracellular distribution and uptake mechanisms of PAEs is still lacking. In this study, a series of hydroponic experiments using intact pumpkin (Cucurbita moschata) seedlings was conducted to investigate how di-n-butyl phthalate (DnBP), one of the most frequently identified PAEs in the environment, enters and is distributed in roots. DnBP was transported into subcellular tissues rapidly in the initial uptake period (<12 h). More than 80% of DnBP was detected in the cell walls and organelles, which suggests that DnBP is primarily accumulated in these two fractions due to their high affinity to DnBP. The kinetics of DnBP uptake were fitted well with the Michaelis-Menten equation, suggesting that a carrier-mediated process was involved. The application of 2,4-dinitrophenol and sodium vanadate reduced the uptake of DnBP by 37 and 26%, respectively, while aquaporin inhibitors, silver and glycerol, had no effect on DnBP uptake. These data demonstrated that the uptake of DnBP included a carrier-mediated and energy-dependent process without the participation of aquaporins.

  18. The Search for Molecular Prognostic Markers of Diabetic Nephropathy in Patients with Type 2 Diabetes Mellitus

    Directory of Open Access Journals (Sweden)

    V. M. Ibragimov

    2016-03-01

    Full Text Available The purpose of this study was to search for molecular prognostic markers of diabetic nephropathy (DN in patients with type 2 diabetes mellitus (T2DM. The study included 205 patients with T2DM and DN (stages 1 to 4. All patients were stratified by the MDRD equation. The control group included 30 healthy individuals. All T2DM patients were divided into 4 groups depending on the DN stages. Group 1 included 42 patients with DN-Stage 1 (prenephropathy, Group 2 included 48 patients with DN-Stage 2 (incipient nephropathy; Group 3 included 65 patients with DN-Stage 3 (overt nephropathy, and Group 4 included 50 patients with DN-Stage 4 (kidney failure. Molecular phenotyping of urine was processed with methods of proteomics: the prefractionation, the separation of proteins with standard sets (MB-HIC C8 Kit, MB-IMAC Cu, MB-Wax Kit, «Bruker», USA, matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS, Ultraflex II, «Bruker», USA. The data of the molecular interactions and functional features of proteins were received with STRING 10.0 database. Potentially new molecular markers of DN development were identified. The research into signaling pathways and the molecules that are involved in ECM formation may help in developing strategies to prevent DN.

  19. Dicty_cDB: Contig-U12705-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Cm_mx0_66d02_SP6 Green Shore Crab Multiple Tissue... 72 2e-18 3 ( DN796200 ) Cm_mx0_60a08_SP6 Green Shore Cr...ab Multiple Tissue... 72 2e-18 3 ( DN796060 ) Cm_mx0_58c06_SP6 Green Shore Crab Multiple Tissue... 72 3e-18 ...3 ( DN796607 ) Cm_mx0_65d03_SP6 Green Shore Crab Multiple Tissue... 72 3e-18 3 ( ...7978 ) Dictyostelium discoideum slug cDNA, clone SSH118. 86 2e-18 3 ( DN796682 )

  20. Rapid Identification of Potential Drugs for Diabetic Nephropathy Using Whole-Genome Expression Profiles of Glomeruli

    Directory of Open Access Journals (Sweden)

    Jingsong Shi

    2016-01-01

    Full Text Available Objective. To investigate potential drugs for diabetic nephropathy (DN using whole-genome expression profiles and the Connectivity Map (CMAP. Methodology. Eighteen Chinese Han DN patients and six normal controls were included in this study. Whole-genome expression profiles of microdissected glomeruli were measured using the Affymetrix human U133 plus 2.0 chip. Differentially expressed genes (DEGs between late stage and early stage DN samples and the CMAP database were used to identify potential drugs for DN using bioinformatics methods. Results. (1 A total of 1065 DEGs (FDR 1.5 were found in late stage DN patients compared with early stage DN patients. (2 Piperlongumine, 15d-PGJ2 (15-delta prostaglandin J2, vorinostat, and trichostatin A were predicted to be the most promising potential drugs for DN, acting as NF-κB inhibitors, histone deacetylase inhibitors (HDACIs, PI3K pathway inhibitors, or PPARγ agonists, respectively. Conclusion. Using whole-genome expression profiles and the CMAP database, we rapidly predicted potential DN drugs, and therapeutic potential was confirmed by previously published studies. Animal experiments and clinical trials are needed to confirm both the safety and efficacy of these drugs in the treatment of DN.

  1. Catalog of Audiovisual Productions. Volume 2. Navy and Marine Corps Productions

    Science.gov (United States)

    1984-06-01

    LAWS AND WAYS OF BUDGET WORK-UP 25281-DN POSTGRADUATE WAY, THE LEARNING (PART 82) 4 -009-DN BUYER "EiARE 25571-DN LIBRARY ASSISTANT. THE 8201-ION...WATER CUEXICAL TREATMENT BUT WILL IT I (REVISED) % r2m (2457 N) (3542701) (IAK NIDO RELEASE- LOFT IA .3 BUYER BEWARE __BLCK OS STLE AIR INNR WATER (AWIG... COMPULSIVE GAMBLER, AND HOW HE AVAILABILITY FROM THE INVENTORY CONTROL POINT FOR EVENTUALLY FACES HIS PROBLEM.21981.DN BASIC PRINCIPLES OF POWFR THE NAVY

  2. Involvement of the NLRC4-Inflammasome in Diabetic Nephropathy

    Science.gov (United States)

    Yuan, Fang; Kolb, Ryan; Pandey, Gaurav; Li, Wei; Sun, Lin; Liu, Fuyou; Sutterwala, Fayyaz S.; Liu, Yinghong; Zhang, Weizhou

    2016-01-01

    Diabetic nephropathy (DN) is the leading cause of end-stage kidney disease worldwide but current treatments remain suboptimal. The role of inflammation in DN has only recently been recognized. It has been shown that the NLRP3-inflammasome contributes to DN development by inducing interleukin (IL)-1β processing and secretion. In an effort to understand other IL-1β activating mechanism during DN development, we examined the role of the NLRC4-inflammasome in DN and found that NLRC4 is a parallel mechanism, in addition to the NLRP3-inflammasome, to induce pro-IL-1β processing and activation. We found that the expression of NLRC4 is elevated in DN kidneys. NLRC4-deficiency results in diminished DN disease progression, as manifested by a decrease in blood glucose and albumin excretion, as well as preserved renal histology. We further found that DN kidneys have increased F4/80+ macrophages, increased IL-1β production, and other signaling pathways related to kidney pathology such as activation of NF-κB and MAP kinase pathways, all of which were rescued by NLRC4-deficiency. This study demonstrates NLRC4-driven IL-1β production as critical for the progression of DN, which underscores the importance to target this pathway to alleviate this devastating disease. PMID:27706238

  3. Association of Chemerin and Vascular Endothelial Growth Factor (VEGF) with Diabetic Nephropathy

    Science.gov (United States)

    Lin, Shuhua; Teng, Jian; Li, Jixia; Sun, Fang; Yuan, Dong; Chang, Jing

    2016-01-01

    Background Diabetic nephropathy (DN) is a common complication of diabetes, caused by diabetic microvascular lesions. The pathogenesis of DN is complicated, involving genetics, physics, chemistry, and environmental factors. Chemerin is a fat cell factor that participates in regulating inflammation. Vascular endothelial growth factor (VEGF) promotes vascular endothelial cell proliferation, differentiation, and angiogenesis. The relationship role of Chemerin and VEGF in DN is not fully understood. Material/Methods SD rats were randomly divided into 2 groups: the control group and the DN group. Streptozotocin was used to construct the DN model. Serum creatinine (Scr), blood urea nitrogen (BUN), and urine microalbumin (UAlb) were detected. Real-time PCR and Western blot were used to test Chemerin and VEGF mRNA and protein expression in kidney tissue. ELISA was performed to test TGF-β1, TNF-α, and INF-γ levels. The correlation of Chemerin and VEGF with renal function and inflammatory factors was analyzed. Results DN group rats showed obviously increased Scr and BUN levels, and elevated TGF-β1, TNF-α, and INF-γ secretion (P<0.05). Compared with controls, Chemerin and VEGF were clearly overexpressed in the DN group (P<0.05). Chemerin and VEGF expression were positively correlated with inflammatory factors and renal function. Conclusions Chemerin and VEGF play important roles in DN by regulating inflammatory factors and renal function. They may be treated as indicators of DN. PMID:27612613

  4. New Insight into the Molecular Drug Target of Diabetic Nephropathy

    Directory of Open Access Journals (Sweden)

    Vivian Soetikno

    2014-01-01

    Full Text Available Diabetic nephropathy (DN lowered quality of life and shortened life expectancy amongst those affected. Evidence indicates interaction between advanced glycation end products (AGEs, activated protein kinase C (PKC and angiotensin II exacerbate the progression of DN. Inhibitors of angiotensin-converting enzyme (ACEIs, renin angiotensin aldosterone system (RAAS, AGEs, and PKC have been tested for slowing down the progression of DN. The exact molecular drug targets that lead to the amelioration of renal injury in DN are not well understood. This review summarizes the potential therapeutic targets, based on putative mechanism in the progression of the disease.

  5. Unigene BLAST: CBRC-RNOR-05-0200 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-05-0200 gnl|UG|Rn#S14182168 UI-R-DN1-cmu-o-17-0-UI.s1 UI-R-DN1 Rattus nor...vegicus cDNA clone UI-R-DN1-cmu-o-17-0-UI 3', mRNA sequence /clone=UI-R-DN1-cmu-o-17-0-UI /clone_end=3' /gb=BQ203707 /gi=20420172 /ti=154456247 /ug=Rn.150870 /len=609 5.0 29% ...

  6. Unigene BLAST: CBRC-RNOR-10-0161 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-10-0161 gnl|UG|Rn#S14182280 UI-R-DN1-cmu-d-23-0-UI.s1 UI-R-DN1 Rattus nor...vegicus cDNA clone UI-R-DN1-cmu-d-23-0-UI 3', mRNA sequence /clone=UI-R-DN1-cmu-d-23-0-UI /clone_end=3' /gb=BQ203819 /gi=20420284 /ti=154456370 /ug=Rn.213369 /len=732 5e-15 47% ...

  7. Unigene BLAST: CBRC-RNOR-06-0132 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-06-0132 gnl|UG|Rn#S14182107 UI-R-DN1-cmu-e-01-0-UI.s1 UI-R-DN1 Rattus nor...vegicus cDNA clone UI-R-DN1-cmu-e-01-0-UI 3', mRNA sequence /clone=UI-R-DN1-cmu-e-01-0-UI /clone_end=3' /gb=BQ203646 /gi=20420111 /ti=154456179 /ug=Rn.34837 /len=711 2.0 29% ...

  8. Unigene BLAST: CBRC-RNOR-09-0129 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-09-0129 gnl|UG|Rn#S14182238 UI-R-DN1-cmu-m-06-0-UI.s1 UI-R-DN1 Rattus nor...vegicus cDNA clone UI-R-DN1-cmu-m-06-0-UI 3', mRNA sequence /clone=UI-R-DN1-cmu-m-06-0-UI /clone_end=3' /gb=BQ203777 /gi=20420242 /ti=154456325 /ug=Rn.153233 /len=788 1e-31 78% ...

  9. Relationship between E-cadherin and diabetic nephropathy

    Institute of Scientific and Technical Information of China (English)

    姜洪娟

    2014-01-01

    Objective To identify novel biomarker for diabetic nephropathy(DN)by urinary proteomic methods,and to detect the expression of E-cadherin in urine and renal tissue of patients with DN.Methods Urine samples were collected from 12 cases of type 1 diabetic nephropathy patients(T1DN),12 cases of type 2 diabetic nephropathy patients(T2DN),12 cases of nephritic syndrome patients(NS),and 12 cases of healthy Controls.Comparative proteomic approach of two-dimensional gel electro-

  10. ОСОБЕННОСТИ ХИМИЧЕСКОГО СОСТАВА МЕРТВОГО МОРЯ И СВОЙСТВА КОМПЛЕКСА DN-1

    OpenAIRE

    Лопатина, А.Б.

    2015-01-01

    В данном научном обзоре рассматриваются особенности химического состава Мертвого моря. Описываются этапы происхождения, формирования и развития Мертвого моря. Приводится сравнительный анализ химического состава Мертвого моря и других соленых водоемов Земли. Раскрывается уникальность химического состава и физические факторы, этому способствующие. Показано, как происходило зарождение жизни на Земле. Описывается, как уникальный химический состав Мертвого моря повлиял на механизмы зарождения и ст...

  11. Construction of eukaryotic expression vector pcDNA 3 .1 (+)-GFP-MC1R and its expression in alpaca hair follicle stem cells%真核表达载体 pcDN A3.1(+)-G FP-M C1R 的构建及在羊驼毛囊干细胞中的表达

    Institute of Scientific and Technical Information of China (English)

    白瑞; 王振华; 尹志红; 弓慧敏; 于志慧; 庞全海

    2015-01-01

    旨在构建含有 MC1R基因的pcDNA3.1(+)真核重组表达载体,为进一步研究 MC1R的生物学功能奠定基础。应用RT‐PCR技术从羊驼皮肤cDNA文库中扩增 MC1R基因全长cDNA ,然后通过双酶切将 MC1R基因全长cDNA克隆到真核表达载体pcDNA3.1(+),然后采用脂质体法转染体外培养的羊驼毛囊干细胞后,通过G418筛选培养,建立稳定的转染体系,直接荧光观察pcDNA3.1/MC1R融合蛋白在细胞中的分布定位,并通过Western Blot方法检测 MC1R在羊驼毛囊干细胞中的表达。结果表明,成功构建了pcDNA3.1/MC1R重组表达载体,并且发现试验组的毛囊干细胞中表达 MC1R蛋白显著高于空白组(P<0.05)。成功构建了含有绿色荧光蛋白基因的真核表达载体pcDNA3.1/MC1R ,且 MC1R基因在羊驼毛囊干细胞中获得表达。%It is the base of further study on MC1R biological functions to construct the pcD‐NA3 .1 eukaryotic expression vector of MC1R gene in alpaca .MC1R gene was amplified by RT‐PCR with the cDNA from alpaca skin library .PCR products and pcDNA3 .1 plasmid were digested and recycled by BamHI and EcoRI endonuclease .The positive clones were selected , from which plasmid DNA was abstracted and identified by restriction endonuclease ,sequence identification and sequencing . The pcDNA3 .1/MC1R recombinant expression plasmid was transfected into alpaca hair follicle stem cells by Lipofectamine TM 2000 .After screening culture by G418 ,a stably‐transfected cell system was established .Fluorescent microscopy was em‐ployed to reveal the localization of MC1R in alpaca hair follicle stem cells ,and the transcrip‐tion and expression of the MC1R were identified by Western Blot .The results show that eu‐karyotic expression vector pcDNA3 .1/MC1R was successfully constructed .When pcDNA3 . 1/MC1R recombinant expression plasmids were transfected into alpaca hair follicle stem cells , the expression of MC1R was up‐regulated (P<0 .05) .This study successfully constructed eu‐karyotic expression vector containing green fluorescent protein gene pcDNA 3 .1/MC1R .It could up‐regulate the expression of MC1R in alpaca hair follicle stem cells .

  12. [Participation of hospital nephrology departments in outpatient dialysis care. Joint Statement of the »Deutsche Gesellschaft für Nephrologie (DGfN)«, »Verband leitender Klinikärzte in der Nephrologie (VLKN)«, »Verband Deutsche Nierenzentren (DN) e. V.« , »KfH Kuratorium für Dialyse und Nierentransplantation e. V.« and »PHV - Der Dialysepartner Patienten-Heimversorgung«].

    Science.gov (United States)

    Floege, Jürgen; Alscher, Mark Dominik; Bach, Dieter; Dähne, Werner; Daschner, Michael; Kuhlmann, Martin

    2015-09-01

    The proposals by health care providers to impose drastic limits on chronic dialysis in hospitals to the extent that it can only be provided on loss-making terms, will jeopardize the cost efficiency of nephrological departments in hospitals and hence their continued existence. Such departments play a key role within the discipline, however, as the training of nephrologists is tied to them by further training regulations. The authors take the view that the proposals by health care providers are short-sighted with regard to the quality of care and the safeguarding of care in the future, and that they counteract the goals of quality assurance currently dominating the health policy agenda. © Georg Thieme Verlag KG Stuttgart · New York.

  13. Serum Hepcidin Predicts Uremic Accelerated Atherosclerosis in Chronic Hemodialysis Patients with Diabetic Nephropathy

    Institute of Scientific and Technical Information of China (English)

    Han Li; Su-Juan Feng; Lu-Lu Su; Wei Wang; Xiao-Dong Zhang; Shi-Xiang Wang

    2015-01-01

    Background:Hepcidin,as a regulator of body iron stores,has been recently discovered to play a critical role in the pathogenesis of anemia of chronic disease.Atherosclerotic cardiovascular disease is the most common complication and the leading cause of death in chronic hemodialysis (CHD) patients.In the current study,we aimed to explore the relationship between serum hepcidin and uremic accelerated atherosclerosis (UAAS) in CHD patients with diabetic nephropathy (CHD/DN).Methods:A total of 78 CHD/DN and 86 chronic hemodialyzed nondiabetic patients with chronic glomerulonephritis (CHD/non-DN) were recruited in this study.The level of serum hepcidin-25 was specifically measured by liquid chromatography-tandem mass spectrometry.Serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent assay.Results:High serum level ofhepcidin-25 was seen in CHD patients.Serum hepcidin-25 in CHD/DN was significantly higher than that in CHD/non-DN patients.Serum hepcidin-25 was positively correlated with ferritin,high-sensitivity C-reactive protein (hs-CRP),TNF-α,and IL-6 in CHD/DN patients.CHD/DN patients exhibited higher common carotid artery intima media thickness (CCA-IMT),hs-CRP,and hepcidin-25 levels than that in CHD/non-DN patients.Moreover,in CHD/DN patients,CCA-IMT was positively correlated with serum hepcidin,hs-CRP,and low-density lipoprotein-cholesterol.On multiple regression analysis,serum hepcidin and hs-CRP level exhibited independent association with IMT in CHD/DN patients.Conclusions:These findings suggest possible linkage between iron metabolism and hepcidin modulation abnormalities that may contribute to the development of UAAS in CHD/DN patients.

  14. Kinetic characterization of steroid 1,2-dehydrogenase from a new Mycobacterium sp mutant of industrial interest

    Directory of Open Access Journals (Sweden)

    Alina Falero

    2004-01-01

    Full Text Available Se realizó un estudio cinético de la enzima esteroide 1,2-deshidrogenasa con células enteras de la cepa 6323, la cual fue aislada por mutagénesis-UV a partir de Ex4, mutante derivada de Mycobacterium NRRL-B3683, obtenida previamente por mutagénesis-UV a partir de un aislado del suelo. La esteroide 1,2-deshidrogenasa es una enzima clave para la biotransformación del sistema de anillos de estos compuestos, la cual es esencial en el metabolismo microbiano de los esteroides. Con anterioridad se ha demostrado que esta enzima es inducible y necesita de un aceptor electrónico externo para la transferencia de electrones desde la enzima hasta el oxígeno molecular, el aceptor final de los electrones liberados en la 1,2 deshidrogenación. Se determinaron las constantes cinéticas de Michaelis-Menten (Kmapa y Vmax.apa. Los valores obtenidos fueron 6,92 · 10-4 mol/L y 2,3 U/mg de biomasa seca, respectivamente. Se estableció el efecto de la concentración de biomasa y la concentración del azul de metileno empleado como aceptor electrónico externo en la reacción enzimática. La actividad máxima se observó entre 5-10 mg/mLde biomasa seca y 1,34 ·10-4 mol/L de azul de metileno. Los resultados obtenidos con azul de metileno, claramente indican la necesidad del uso del aceptor en la 1,2 deshidrogenación de esteroides, como ha sido reportado anteriormente por otros autores. Además se estudió el efecto del pH sobre la velocidad inicial de reacción, obteniéndose la actividad máxima en el intervalo de 8,4-8,6. Los resultados del estudio confirman que esta nueva cepa posee una actividad esteroide 1,2-deshidrogenasa superior con relación a sus parentales. Esto se hace evidente de acuerdo con los parámetros cinéticos obtenidos, los cuales son de gran interés desde el punto de vista industrial.

  15. Dicty_cDB: Contig-U10437-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available me Vinous Sweet Orang... 44 3.4 1 ( DN625586 ) UCRCA01_06K11_f Bark of Madame Vinou... T21L20TR TAMU Arabidopsis thaliana genomic clone T2... 44 3.4 1 ( DN625587 ) UCRCA01_06K11_r Bark of Mada

  16. Dicty_cDB: Contig-U11290-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available oem Citrus sinen... 48 1.0 1 ( DN623638 ) UCRCA01_03N07_r Bark of Madame Vinous S...weet Orang... 48 1.0 1 ( DN623637 ) UCRCA01_03N07_f Bark of Madame Vinous Sweet Orang... 48 1.0 1 ( BJ340097

  17. Dicty_cDB: Contig-U16329-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 34 5.6 2 ( DN622028 ) UCRCA01_01I19_f Bark of Madame Vinous Sweet Orang... 34 5.6... 2 ( CV884734 ) UCRCS04_2_010C05_T3 Ruby Orange Developing Flower... 34 5.6 2 ( DN622029 ) UCRCA01_01I19_r Bark of Madame

  18. Suppressing autoimmunity in Arabidopsis thaliana with dominant negative immune receptors

    DEFF Research Database (Denmark)

    Greeff, Michael Christiaan

    2014-01-01

    R genes, as we have recently found that transgenics with similarly mutated rpm1-DN alleles lose resistance to Pseudomonas syringae expressing the AvrRpm1 effector. Accordingly, we have constructed a collection of 100 R-DN alleles and transformed them into other autoimmune mutants including camta3...

  19. 76 FR 72439 - Certain Consumer Electronics and Display Devices and Products Containing Same; Receipt of...

    Science.gov (United States)

    2011-11-23

    ...-72440] [FR Doc No: 2011-30184] INTERNATIONAL TRADE COMMISSION [DN 2858] Certain Consumer Electronics and... Consumer Electronics and Display Devices and Products Containing Same, DN 2858; the Commission is... importation of certain consumer electronics and display devices and products containing same. The...

  20. HLA-DR, and not PLA2R, is expressed on the podocytes in kidney allografts in de novo membranous nephropathy.

    Science.gov (United States)

    Wen, Jiqiu; Xie, Kenan; Zhang, Mingchao; Chen, Jinsong; Zhang, Jiong; Cheng, Dongrui; Li, Xue; Ji, Shuming; Liu, Zhihong

    2016-09-01

    Idiopathic membranous nephropathy (IMN) is known to be associated with antibodies acting on the M-type phospholipase A2 receptor (PLA2R) of the podocyte. However, the mechanism underlying de novo membranous nephropathy (dn MN) posttransplantation remains unclear. In this study, we aimed to elucidate the mechanism underlying dn MN.We selected 8 cases with dn MN and compared them to 20 IMN cases. Fifteen cases of stable grafts were selected as controls.Several differences between the dn MN group and the IMN group were detected. IgG4 showed negligible positive staining in patients with dn MN, while it was predominant in the IMN group (1/8 vs 20/20, P PLA2R antibodies and anti-PLA2R antibodies of the podocyte were very few in the dn MN patients; however, these antibodies were detected in most of the IMN patients (serum anti-PLA2R antibodies: 1/8 vs 16/20, P = 0.002, anti-PLA2R antibodies of the podocyte: 0/8 vs 17/20, P PLA2R pathway, which is known to play a role in IMN, was not involved in the mechanism underlying dn MN. On the contrary, dn MN might be associated with the alloimmune response directed against the podocyte.

  1. Protective effects of bendazac lysine on early experimental diabetic nephropathy in rats

    Institute of Scientific and Technical Information of China (English)

    Xiao-xing YIN; Yin-di ZHANG; Jian-ping SHEN; Hai-wei WU; Xiang ZHU; Li-min LI; Jun QIU; Shao-jun JIANG; Xiao-gang ZHENG

    2005-01-01

    Aim: To investigate the preventive and protective effects of bendazac lysine(BDL) on experimental early diabetic nephropathy (DN) rats. Methods: After an early DN model was induced by streptozotocin, rats were administered BDL at doses of 100, 200, and 400 mg/kg for 8 weeks. Blood glucose, microalbuminuria,kidney index, total antioxidative capacity, laminin, advanced glycation end products (AGE), aldose reductase (AR) activity, and the relative quantity of transforming growth factor β1 (TGF-β1) mRNA were measured by different methods.The ultrastructural morphology was observed by transmission electron microscope. Results: The physical behaviors of early DN rats were hypopraxia,cachexia, and polyuria, while those treated with high doses of BDL were vibrant and vigorous. For BDL-treated DN rats, when compared with vehicle-treated DN rats, the blood glucose level and the intensity of oxidative stress were ameliorated.Also, the microalbuminuria level, AGE either in serum or in renal, and AR activity were significantly reduced. Furthermore, the expression of TGF-β1 mRNA in the kidney cortex was declined and the thickness of glomerular base membrane was decreased significantly. The ultrastructure of glomerulus and mesangial matrix of BDL-treated DN rats were ameliorated. Conclusion: BDL has protective effects on several pharmacological targets in the progress of DN and is a potential drug for the prevention of early DN.

  2. VP22 fusion protein-based dominant negative mutant can inhibit hepatitis B virus replication

    Institute of Scientific and Technical Information of China (English)

    Jun Yi; Wei-Dong Gong; Ling Wang; Rui Ling; Jiang-Hao Chen; Jun Yun

    2005-01-01

    AIM: To investigate the inhibitory effect of VP22 fusion protein-based dominant negative (DN) mutant on Hepatitis Bvrus (HBV) replication.METHODS: Full-length or truncated fragment of VP22 was fused to C terminal of HBV core protein (HBc), and subcloned into pcDNA3.1 (-) vector, yielding eukaryotic expression plasmids of DN mutant. After transfection into HepG2.2.15 cells, the expression of DN mutant was identified by immunofluorescence staining. The inhibitory effect of DN mutant on HBV replication was indexed as the supernatant HBsAg concentration determined by RIA and HBV-DNA content by fluorescent quantification-PCR (FQ-PCR). Meanwhile, metabolism of HepG2.2.15 cells was evaluated by MTT colorimetry.RESULTS: VP22-based DN mutants and its truncated fragment were expressed in HepG2.2.15 cells, and had no toxic effect on host cells. DN mutants could inhibit HBV replication and the transduction ability of mutantbearing protein had a stronger inhibitory effect on HBV replication. DN mutants with full length of VP22 had the strongest inhibitory effect on HBV replication, reducing the HBsAg concentration by 81.94%, and the HBV-DNA content by 72.30%. MTT assay suggested that there were no significant differences in cell metabolic activity between the groups.CONCLUSION: VP22-based DN mutant can inhibit HBV replication effectively.

  3. New susceptibility loci associated with kidney disease in type 1 diabetes

    DEFF Research Database (Denmark)

    Sandholm, Niina; Salem, Rany M; McKnight, Amy Jayne

    2012-01-01

    Diabetic kidney disease, or diabetic nephropathy (DN), is a major complication of diabetes and the leading cause of end-stage renal disease (ESRD) that requires dialysis treatment or kidney transplantation. In addition to the decrease in the quality of life, DN accounts for a large proportion of ...

  4. Dicty_cDB: Contig-U05480-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available ... 42 3e-05 2 ( AU268504 ) Dictyostelium discoideum vegetative cDNA clone:VS... 60 1e-04 1 ( DN778026 ) E5946 [C3917|slt10_g05] Sali...nity Library Thellung... 58 5e-04 1 ( DN775170 ) E5211 [C772|cld9_a04] Cold Library

  5. Statistics and characteristics of xuv transition arrays from laser-produced plasmas of the elements tin through iodine

    DEFF Research Database (Denmark)

    Svendsen, Winnie Edith; O'Sullivan, G.

    1994-01-01

    Spectra of laser-produced plasmas of the elements from tin to iodine contain weak bands of quasicontinuum overlaid by weak emission lines in the 70–120-Å region. Multiconfiguration-Dirac-Fock calculations show that these features are consistent with theoretical spectra for 4dN-4dN-1(5f+6p) transi...

  6. Dicty_cDB: Contig-U13901-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 4 3e-22 7 ( Z74319 ) S.cerevisiae chromosome IV reading frame ORF YDR023w. 46 8e-22 8 ( DN740372 ) 8 Cassa...va root cDNA library Manihot esculenta cDN... 76 3e-21 3 ( DY647026 ) PU3_plate36_F

  7. Dicty_cDB: SFK782 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 24. 652 0.0 1 ( EH431480 ) NPE00000879 Neocallimastix patriciarum ZAP II cDN... 141 8e-49 2 ( DY885764 ) Cel...s pBluescript (E... 139 1e-43 2 ( EH432023 ) NPE00000423 Neocallimastix patriciarum ZAP II cDN... 94 4e-41 3

  8. The Philosophy of Science and Technology in China: Political and Ideological Influences

    Science.gov (United States)

    Guo, Yuanlin

    2014-01-01

    In China, the philosophy of science and technology (PST) is derived from "Dialectics of Nature" (DN), which is based on Engels' unfinished book "Dialektik der Natur." DN as a political ideology provides political guidance for scientists and engineers. Therefore, since 1981, "Introduction to Dialectics of Nature"…

  9. Double negative regulatory T cells in transplantation and autoimmunity: recent progress and future directions

    Institute of Scientific and Technical Information of China (English)

    Stephen C. Juvet; Li Zhang

    2012-01-01

    T lymphocytes bearing the αβ T cell receptor (TCR) but lacking CD4,CD8,and markers of natural killer (NK) cell differentiation are known as ‘double-negative’ (DN) T cells and have been described in both humans and rodent models.We and others have shown that DN T cells can act as regulatory T cells (Tregs) that are able to prevent allograft rejection,graft-versus-host disease,and autoimmune diabetes.In the last few years,new data have revealed evidence of DN Treg function in vivo in rodents and humans.Moreover,significant advances have been made in the mechanisms by which DN Tregs target antigen-specific T cells.One major limitation of the field is the lack of a specific marker that can be used to distinguish truly regulatory DN T cells (DN Tregs) from non-regulatory ones,and this is the central challenge in the coming years.Here,we review recent progress on the role of DN Tregs in transplantation and autoimmunity,and their mechanisms of action.We also provide some perspectives on how DN Tregs compare with Foxp3+ Tregs.

  10. Abstract Central neuropathic mechanisms in post-stroke shoulder pain

    NARCIS (Netherlands)

    Roosink, M.; Renzenbrink, G.J.; Buitenweg, Jan R.; Van Dongen, R.T.M.; Geurts, A.C.H.; IJzerman, Maarten Joost

    2009-01-01

    Central neuropathic pain mechanisms may play a larger role in chronic PSSP than is traditionally assumed. With the DN4, it is possible to select a subgroup of PSSP patients with more severe pain complaints and sensory abnormalities. Subgroup identification based on the DN4 might be used in future

  11. Immediate and short-term effects of the combination of dry needling and percutaneous TENS on post-needling soreness in patients with chronic myofascial neck pain.

    Science.gov (United States)

    León-Hernández, Jose V; Martín-Pintado-Zugasti, Aitor; Frutos, Laura G; Alguacil-Diego, Isabel M; de la Llave-Rincón, Ana I; Fernandez-Carnero, Josue

    2016-07-11

    Dry needling (DN) and percutaneous electrical nerve stimulation (PENS) are widely used techniques in the treatment of myofascial pain. To investigate the immediate and short-term effects of the combination of DN and PENS compared to DN alone on the upper trapezius muscle. This is a 72-hour follow-up single-blinded randomized controlled trial. Sixty-two volunteer patients with chronic myofascial neck pain with active Myofascial Trigger Points (MTrPs) in the upper trapezius muscle were recruited. Randomization was performed, and 31 patients received DN treatment (DN group) and 31 received DN and PENS (DN+PENS group). The primary outcomes were neck disability index (NDI) and visual analog scale for pain for both post-needling soreness (PNS) and neck pain intensity (NPI). Pressure pain threshold (PPT) and cervical range of motion (CROM) were the secondary outcomes. We detected between-group differences in NPI and PNS in favor of the DN+PENS group immediately after treatment. No between-group differences in NDI were observed. PENS application after dry needling treatment is more effective than dry needling alone for decreasing soreness in the short term and improving neck pain intensity immediately in patients with myofascial chronic neck pain.

  12. Immediate and short-term effects of the combination of dry needling and percutaneous TENS on post-needling soreness in patients with chronic myofascial neck pain

    Directory of Open Access Journals (Sweden)

    Jose V. León-Hernández

    Full Text Available ABSTRACT Background Dry needling (DN and percutaneous electrical nerve stimulation (PENS are widely used techniques in the treatment of myofascial pain. Objective To investigate the immediate and short-term effects of the combination of DN and PENS compared to DN alone on the upper trapezius muscle. Method This is a 72-hour follow-up single-blinded randomized controlled trial. Sixty-two volunteer patients with chronic myofascial neck pain with active Myofascial Trigger Points (MTrPs in the upper trapezius muscle were recruited. Randomization was performed, and 31 patients received DN treatment (DN group and 31 received DN and PENS (DN+PENS group. The primary outcomes were neck disability index (NDI and visual analog scale for pain for both post-needling soreness (PNS and neck pain intensity (NPI. Pressure pain threshold (PPT and cervical range of motion (CROM were the secondary outcomes. Results We detected between-group differences in NPI and PNS in favor of the DN+PENS group immediately after treatment. No between-group differences in NDI were observed. Conclusion PENS application after dry needling treatment is more effective than dry needling alone for decreasing soreness in the short term and improving neck pain intensity immediately in patients with myofascial chronic neck pain.

  13. Trigger-Point Dry Needling for the SOF Medic.

    Science.gov (United States)

    Cavett, Thomas; Solarczyk, Justin

    We propose that trigger-point dry needle (TrP-DN) therapy is an effective low-risk treatment for pain associated with myofascial trigger points (MTrP), and should be incorporated into the Special Operations Forces (SOF) Medic's scope of practice. Furthermore, TrP-DN therapy can be used as a treatment adjunct on the SOF continuum of care, providing analgesia and increased tolerance for rehabilitative therapy, thereby facilitating improved patient outcomes and faster return to operational readiness. The incidence of musculoskeletal injuries in the SOF community is discussed, as are available treatment options TrP-DN methods, a case study of a Soldier deployed to Afghanistan, the science behind the subject of MTrP and TrP-DN, and the risks associated with TrP-DN and how we can mitigate them effectively. Caution should be used in the interpretation of a body of literature based largely on case studies. Although the amount of published evidence in support of the potential benefits of TrP-DN is growing, larger, randomized, placebo-controlled trials and studies that evaluate the effects of TrP-DN in a methodologically rigorous and statistically significant way are needed. Based on anecdotal evidence of and personal experience with the success of the therapy, as well as its growing use within both civilian and military medicine, the possible therapeutic benefit of TrP-DN is relevant for the SOF community. 2016.

  14. Dicty_cDB: Contig-U05050-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available NA, clone:PTB-004N22.R. 46 2.2 1 ( DN551390 ) Cm_mx0_34e01_SP6 Green Shore Crab Multiple Tissue... 46 2.2 1 ...( DN550831 ) Cm_mx0_28b07_SP6 Green Shore Crab Multiple Tissue... 46 2.2 1 ( CX18

  15. Standardizing Methods for Weapons Accuracy and Effectiveness Evaluation

    Science.gov (United States)

    2014-06-01

    O. Yakimenko, Engineering Computations and Modeling in MATLAB / Simulink . Reston, VA: AIAA, 2011. [6] Global Positioning System Standard...CONCLUSIONS AND RECOMMENDATIONS......................85  APPENDIX ....................................................87  A.  MATLAB CODE – DN_CDF_DATA...87  1.  MATLAB CODE DN_myfit.......................89  B.  MATLAB CODE – DR_CDF_DATA.......................89  1.  MATLAB CODE

  16. Bilirubin and Progression of Nephropathy in Type 2 Diabetes : A Post Hoc Analysis of RENAAL With Independent Replication in IDNT

    NARCIS (Netherlands)

    Riphagen, Ineke J.; Deetman, Petronella E.; Bakker, Stephan J. L.; Navis, Gerarda; Cooper, Mark E.; Lewis, Julia B.; de Zeeuw, Dick; Lambers Heerspink, Hiddo

    2014-01-01

    Bilirubin, a potent endogenous antioxidant, was found to protect against the development of diabetic nephropathy (DN) in rodents. In humans, cross-sectional studies found an inverse relation between bilirubin and DN. We prospectively investigated whether bilirubin is associated with progression of D

  17. Double-Negative αβ T Cells Are Early Responders to AKI and Are Found in Human Kidney.

    Science.gov (United States)

    Martina, Maria N; Noel, Sanjeev; Saxena, Ankit; Bandapalle, Samatha; Majithia, Richa; Jie, Chunfa; Arend, Lois J; Allaf, Mohamad E; Rabb, Hamid; Hamad, Abdel Rahim A

    2016-04-01

    Ischemia-reperfusion injury (IRI) is a major cause of AKI, and previous studies established important roles for conventional CD4(+) T cells, natural killer T cells, and CD4(+)CD25(+)FoxP3(+) Tregs in AKI pathogenesis. We recently identified CD4(-)CD8(-) (double-negative; DN) T cells as an important subset of αβ T cell receptor-positive cells residing in mouse kidney. However, little is known about the pathophysiologic functions of kidney DN T cells. In this study, we phenotypically and functionally characterized murine kidney DN T cells in the steady state and in response to IRI. Unlike CD4(+) and CD8(+) T cells, DN T cells in the steady state expressed high levels of CD69, CD28, and CD40L; differentially expressed IL-27 and IL-10 anti-inflammatory cytokines; spontaneously proliferated at a very high rate; and suppressed in vitro proliferation of activated CD4(+) T cells. Within the first 3-24 hours after IRI, kidney DN T cells expanded significantly and upregulated expression of IL-10. In adoptive transfer experiments, DN T cells significantly protected recipients from AKI by an IL-10-dependent mechanism. DN T cells also made up a large fraction of the T cell compartment in human kidneys. Our results indicate that DN T cells are an important subset of the resident αβ(+) T cell population in the mammalian kidney and are early responders to AKI that have anti-inflammatory properties.

  18. Value of Cystatin C in Diagnosis of Renal Injury in Patients with Early stage of diabetes%血清胱抑素C在糖尿病早期肾损伤诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    陈瑶

    2010-01-01

    @@ 糖尿病肾病(diabetic Nephropathy,DN)是糖尿病(diabetics Melitus,DM)常见和严重的并发症之一,临床上以DM患者出现持续性尿蛋白为主要标志,有20%~40%的DM患者最终发展为DN[1].

  19. Elevated Urinary Connective Tissue Growth Factor in Diabetic Nephropathy Is Caused by Local Production and Tubular Dysfunction

    NARCIS (Netherlands)

    Gerritsen, Karin G. F.; Leeuwis, JanWillem; Koeners, Maarten P.; Bakker, Stephan J. L.; van Oeveren, Willem; Aten, Jan; Tarnow, Lise; Rossing, Peter; Wetzels, Jack F. M.; Joles, Jaap A.; Kok, Robbert Jan; Goldschmeding, Roel; Nguyen, Tri Q.

    2015-01-01

    Connective tissue growth factor (CTGF; CCN2) plays a role in the development of diabetic nephropathy (DN). Urinary CTGF (uCTGF) is elevated in DN patients and has been proposed as a biomarker for disease progression, but it is unknown which pathophysiological factors contribute to elevated uCTGF. We

  20. Matrix Metalloproteinase-9 −1562C/T Gene Polymorphism Is Associated with Diabetic Nephropathy

    Science.gov (United States)

    Feng, Shufen; Ye, Gang; Bai, Shi; Liao, Xueling; Li, Lu

    2016-01-01

    To investigate the association between the metalloproteinase-9 (MMP9) −1562C/T polymorphism and diabetic nephropathy (DN) in Han Chinese, the patients with type 2 diabetes were collected and divided into the non-DN (NDN) and DN groups; controls were recruited. Genotype and allele frequencies were assessed using polymerase chain reaction and restriction fragment length polymorphism. Results showed that SBP, DBP, HbA1c, UAER, Cr, BUN, TG, and TC were higher in the DN group compared with the control and NDN groups. SBP, HbA1c, and TC in DN patients with the TT and CT genotypes were lower than in those with CC. Compared with controls, the frequency of the T allele in the DN group was significantly lower. The MMP9 −1562C allele, SBP, Cr, BUN, TG, and TC were independent risk factors for DN. All of the above suggested that the MMP9 −1562C/T polymorphism was associated with DN in Han Chinese. PMID:27631001

  1. Plasmin reduces fibronectin deposition by mesangial cells in a protease-activated receptor-1 independent manner

    Directory of Open Access Journals (Sweden)

    Maaike Waasdorp

    2017-07-01

    Conclusion: Expression profiling identified plasmin as potential endogenous PAR-1 agonist driving DN. Instead of inducing fibronectin expression, plasmin however reduced mesangial fibronectin deposition in vitro. Therefore we conclude that plasmin may not be the endogenous PAR-1 agonist potentiating DN.

  2. A Dwarf Nova in the Globular Cluster M13

    NARCIS (Netherlands)

    Servillat, M.; Webb, N.A.; Lewis, F.; Knigge, C.; van den Berg, M.C.; Dieball, A.; Grindlay, J.E.

    2011-01-01

    Dwarf novae (DNe) in globular clusters (GCs) seem to be rare with only 13 detections in the 157 known Galactic GCs. We report the identification of a new DN in M13, the 14th DN identified in a GC to date. Using the 2 m Faulkes Telescope North, we conducted a search for stars in M13 that show variabi

  3. Fenugreek Prevents the Development of STZ-Induced Diabetic Nephropathy in a Rat Model of Diabetes

    Directory of Open Access Journals (Sweden)

    Yingli Jin

    2014-01-01

    evidently reduced by fenugreek treatment. Furthermore, the upregulation of TGF-β1 and CTGF at a transcriptional and translational level in DN rats was distinctly inhibited by fenugreek. Consequently, fenugreek prevents DN development in a STZ-induced diabetic rat model.

  4. The role of CTGF in diabetic nephropathy : Marker, pathogenic factor and target for therapeutic intervention

    NARCIS (Netherlands)

    Roestenberg, Patricia Maria Henrica

    2007-01-01

    Diabetic nephropathy (DN) is a common complication of diabetes mellitus (DM) and a major cause of end stage renal disease. DN is a progressive fibrotic kidney disease that is structurally characterized by mesangial matrix accumulation and thickening of the glomerular basement membrane (GBM), and fun

  5. Renoprotective effects of the AGE-inhibitor pyridoxamine in experimental chronic allograft nephropathy in rats

    NARCIS (Netherlands)

    Waanders, Femke; van den Berg, Else; Nagai, Ryoji; van Veen, Ingrid; Navis, Gerjan; van Goor, Harry

    2008-01-01

    Background. Advanced glycation end products (AGEs) are involved in diabetic nephropathy (DN). The AGE formation inhibitor pyridoxamine (PM) is renoprotective in DN and in normoglycaemic obese Zucker rats. In chronic allograft nephropathy (CAN), renal AGE accumulation occurs as well. Methods. To inve

  6. Dicty_cDB: Contig-U14511-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available .21 1 ( DR755039 ) USDA-FP_114900-054 adult black-winged sharpshoote... 50 0.21 1... ( DN470438 ) USDA-FP_144428 Adult sharpshooter leafhopper Onco... 50 0.21 1 ( DN197332 ) USDA-FP_134046 5th

  7. Dicty_cDB: CHR719 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available Washington Navel Orange Scale-Infested Rind cDNA Library UCRCS11 Citrus sinensis cDNA clone CS_WEd0004D15, m...7, WORKING DRAFT SEQUENCE. 40 0.40 3 DN619654 |DN619654.1 UCRCS11_04D15_r Parent

  8. Urinary Biomarkers in the Assessment of Early Diabetic Nephropathy

    Directory of Open Access Journals (Sweden)

    Cristina Gluhovschi

    2016-01-01

    Full Text Available Diabetic nephropathy (DN is a frequent and severe complication of diabetes mellitus (DM. Its diagnosis in incipient stages may allow prompt interventions and an improved prognosis. Towards this aim, biomarkers for detecting early DN can be used. Microalbuminuria has been proven a remarkably useful biomarker, being used for diagnosis of DN, for assessing its associated condition—mainly cardiovascular ones—and for monitoring its progression. New researches are pointing that some of these biomarkers (i.e., glomerular, tubular, inflammation markers, and biomarkers of oxidative stress precede albuminuria in some patients. However, their usefulness is widely debated in the literature and has not yet led to the validation of a new “gold standard” biomarker for the early diagnosis of DN. Currently, microalbuminuria is an important biomarker for both glomerular and tubular injury. Other glomerular biomarkers (transferrin and ceruloplasmin are under evaluation. Tubular biomarkers in DN seem to be of a paramount importance in the early diagnosis of DN since tubular lesions occur early. Additionally, biomarkers of inflammation, oxidative stress, podocyte biomarkers, and vascular biomarkers have been employed for assessing early DN. The purpose of this review is to provide an overview of the current biomarkers used for the diagnosis of early DN.

  9. Finding diabetic nephropathy biomarkers in the plasma peptidome by high-throughput magnetic bead processing and MALDI-TOF-MS analysis

    DEFF Research Database (Denmark)

    Hansen, Henning G; Overgaard, Julie; Lajer, Maria

    2010-01-01

    Diabetic nephropathy (DN) is the most common cause of end-stage renal disease and improved biomarkers would help identify high-risk individuals. The aim of this study was to discover candidate biomarkers for DN in the plasma peptidome in an in-house cross-sectional cohort (n=122) of type 1 diabetic...

  10. The Philosophy of Science and Technology in China: Political and Ideological Influences

    Science.gov (United States)

    Guo, Yuanlin

    2014-01-01

    In China, the philosophy of science and technology (PST) is derived from "Dialectics of Nature" (DN), which is based on Engels' unfinished book "Dialektik der Natur." DN as a political ideology provides political guidance for scientists and engineers. Therefore, since 1981, "Introduction to Dialectics of Nature" (IDN)…

  11. ANTIBODY POLYCLONAL PRODUCTION ON RABBIT ANTI-OVINE PREGNANCY-ASSOCIATED GLYCOPROTEIN (Rabbit anti-ovPAG

    Directory of Open Access Journals (Sweden)

    E.T. Setiatin

    2014-10-01

    Full Text Available The aim of the study was to produce polyclonal antibody (rabbit anti-ovPAG which could detectPAG in the urine of pregnant ewes. Twelve rabbits were immunized against ovPG DEAE-TrisHCl (DT,DEAE-NaCl 20mM (DN2, DEAE-NaCl 40mM (DN4, DEAE-NaCl 80mM (DN8, DEAE-NaCl160mM (DN16, DEAE-NaCl 320mM (DN32 and DEAE-NaCl 1M (DN1 and NaCl 0.9 % as aplacebo. The 0.5 ml of isolate (purified from ovine cotyledon was emulsified in equal volume withcomplete and incomplete Freud’s adjuvant. The mixture of each isolate and adjuvant was injected atmutiple sites along the dorsal area of rabbits by subcutaneous route. Blood were collected from marginalear vein, starting before first injection (baseline and every 14 days. Rabbit anti-ovPAG were measuredusing Modified ELISA Technique. By using Western Blot Technique, DN32 showed the best immuneresponse among others and also could differenciate ovPAG in the urine of pregnant ewes It could beconcluded that ovPAG DN32 is a specific source of rabbit anti-ovPAG production. Protein of ovPAG atmolecular weight 31 kDa is a pregnancy protein marker of garut sheep and could be developed as amajor protein for producing antibodi.

  12. Activity-Dependent Excitability Changes Suggest Na[superscript +]/K[superscript +] Pump Dysfunction in Diabetic Neuropathy

    Science.gov (United States)

    Krishnan, Arun V.; Lin, Cindy S.-Y.; Kiernan, Matthew C.

    2008-01-01

    The present study was undertaken to evaluate the role of Na[superscript +]/K[superscript +] pump dysfunction in the development of diabetic neuropathy (DN). Nerve excitability techniques, which provide information about membrane potential and axonal ion channel function, were undertaken in 15 patients with established DN and in 10 patients with…

  13. On conjugacy of MASAs and the outer automorphism group of the Cuntz algebra

    DEFF Research Database (Denmark)

    Conti, Roberto; Hong, Jeong Hee; Szymanski, Wojciech

    2015-01-01

    We investigate the structure of the outer automorphism group of the Cuntz algebra and the closely related problem of conjugacy of MASAs in O_n. In particular, we exhibit an uncountable family of MASAs, conugate to the standard MASA D_n via Bogolubov automorphisms, that are not inner conjugate to D_n....

  14. Lymphocytic profiling in thyroid cancer provides clues for failure of tumor immunity.

    Science.gov (United States)

    Imam, Shahnawaz; Paparodis, Rodis; Sharma, Deepak; Jaume, Juan Carlos

    2014-06-01

    Thyroid cancers are usually surrounded by a significant number of immune-reactive cells. Tumor-associated lymphocytes as well as background lymphocytic thyroiditis are frequently mentioned in pathology reports of patients who have undergone surgery for thyroid cancer. The nature of this lymphocytic reaction is not well understood. The fact that cancer can survive in this adverse microenvironment is indicative of immune regulation. We characterized the lymphocytic infiltration that accompanies thyroid cancer and compared it with that present in thyroid autoimmunity. We found that double-negative (DN) T cells were significantly more abundant in thyroid cancer than in thyroid autoimmunity. Although FOXP3(+) regulatory T cells were also present, DN T cells were the dominant cell type, associated with thyroid cancer. Furthermore, upon stimulation, the DN T cells associated with cancer remained unchanged, while the few (20%). CD25 expression on DN T cells remained unchanged after stimulation, which indicates that the increase in the absolute number of DN T cells in thyroid autoimmunity was at the expense of inactivation of single-positive T cells. We concluded that in the setting of thyroid cancer, DN T cells appear to suppress tumor immunity. In contrast, in thyroid autoimmunity, DN T cells were barely present and only increased at the expense of inactivated, single-positive T cells upon induction. Together, these findings indicate that thyroid cancer-associated DN T cells might regulate proliferation and effector function of T cells and thereby contribute to tumor tolerance and active avoidance of tumor immunity.

  15. NOS3 894G>T polymorphism is associated with progression of kidney disease and cardiovascular morbidity in type 2 diabetic patients: NOS3 as a modifier gene for diabetic nephropathy?

    Science.gov (United States)

    Kuricová, Katarína; Tanhäuserová, Veronika; Pácal, Lukáš; Bartáková, Vendula; Brožová, Lucie; Jarkovský, Jiří; Kaňková, Kateřina

    2013-01-01

    We have previously associated SNP 894G>T in the NOS3 gene with diabetic nephropathy (DN) using multi-locus analysis. Variant 894G>T has been widely studied as a DN susceptibility factor with contradictory results. In the present study we genotyped 894G>T in the cohort of prospectively followed type 2 diabetics with the aim to investigate its possible role in the progression of DN and development of morbidity and mortality associated with diabetes. 311 subjects with defined stage of DN were enrolled in the study and followed up for a median of 38 months. We considered three end-points: progression of DN, major cardiovascular event and all-cause mortality. Considering baseline GFR, age at enrolment and diabetes duration as confounders, Cox regression analysis identified 894GT genotype as a risk factor for DN progression (HR = 1.843 [95% CI 1.088 - 3.119], P = 0.023) and 894TT genotype as a risk factor for major cardiovascular event (HR = 2.515 [95% CI 1.060 - 5.965], P = 0.036). We ascertained the significant effect of the NOS3 894G>T variant on DN progression and occurrence of major cardiovascular event in T2DM subjects. Based on these results NOS3 can be considered a modifier gene for DN.

  16. Renoprotective effects of the AGE-inhibitor pyridoxamine in experimental chronic allograft nephropathy in rats

    NARCIS (Netherlands)

    Waanders, Femke; van den Berg, Else; Nagai, Ryoji; van Veen, Ingrid; Navis, Gerjan; van Goor, Harry

    2008-01-01

    Background. Advanced glycation end products (AGEs) are involved in diabetic nephropathy (DN). The AGE formation inhibitor pyridoxamine (PM) is renoprotective in DN and in normoglycaemic obese Zucker rats. In chronic allograft nephropathy (CAN), renal AGE accumulation occurs as well. Methods. To inve

  17. The Philosophy of Science and Technology in China: Political and Ideological Influences

    Science.gov (United States)

    Guo, Yuanlin

    2014-01-01

    In China, the philosophy of science and technology (PST) is derived from "Dialectics of Nature" (DN), which is based on Engels' unfinished book "Dialektik der Natur." DN as a political ideology provides political guidance for scientists and engineers. Therefore, since 1981, "Introduction to Dialectics of Nature" (IDN)…

  18. Decoupling of structural and functional brain connectivity in older adults with white matter hyperintensities

    NARCIS (Netherlands)

    Reijmer, Y. D.; Schultz, A. P.; Leemans, A.; O'Sullivan, M. J.; Gurol, M. E.; Sperling, R.; Greenberg, S. M.; Viswanathan, A.; Hedden, T.

    2015-01-01

    Age-related impairments in the default network (DN) have been related to disruptions in connecting white matter tracts. We hypothesized that the local correlation between DN structural and functional connectivity is negatively affected in the presence of global white matter injury. In 125 clinically

  19. Dicty_cDB: Contig-U09147-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 6761 ) G.hir-20, 30 hrs post inoculation 71 Leaf cDNA 20... 34 0.54 2 ( DN826776 ) G.hir-20, 30 hrs post inoculation... 86 Leaf cDNA 20... 34 0.54 2 ( DN826715 ) G.hir-20, 30 hrs post inoculation

  20. Dicty_cDB: SHE759 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available m raimondii cDNA clone GR__Eb0034H20 3', mRNA sequence. 42 3e-14 5 DN826070 |DN826070.1 G.hir-8,14 hrs post inoculation... 13 Leaf cDNA 8,14 hrs post inoculation with Xanthomonas campestris pv. malvacearum 3631 Gossypiu

  1. Association of peroxisome proliferator-activated receptor γ Pro12Ala gene polymorphism with type 2 diabetic nephropathy risk in Caucasian population.

    Science.gov (United States)

    Zhou, Tian-Biao; Guo, Xue-Feng; Yin, Sheng-Sheng

    2014-06-01

    Association of peroxisome proliferator-activated receptor gamma (PPARγ) Pro12Ala gene polymorphism with type 2 diabetic nephropathy (T2DN) risk in Caucasians is still not clear. This investigation was conducted to assess if there was an association between the PPARγ Pro12Ala gene polymorphism and T2DN risk in Caucasians using meta-analysis. The relevant literatures were identified from PubMed, and Cochrane Library on 10 October 2013, and eligible studies were included and synthesized. Six reports including eight studies were recruited into this meta-analysis for the association of the PPARγ Pro12Ala gene polymorphism with T2DN risk in Caucasians. The Pro/Pro genotype was shown to be associated with T2DN risk in Caucasians. However, the Ala/Ala genotype and Ala allele were not associated with T2DN risk in Caucasians. In the sensitivity analysis, according to the control source from hospital, the control source from population, the genotyping methods using PCR-RFLP, Taqman, sample size of case Pro12Ala gene polymorphism with T2DN risk was similar to those in non-sensitivity analysis. In conclusion, the PPARγ Pro/Pro genotype was associated with T2DN risk in Caucasians, but the Ala/Ala genotype and Ala allele not. However, additional studies are required to firmly establish a correlation between the PPARγ Pro12Ala gene polymorphism and T2DN risk in Caucasians.

  2. Languages of Iran: Past and Present - Iranian Studies in memoriam David Neil MacKenzie / Dieter Weber (ed.). Wiesbaden : Harrassowitz, 2005

    DEFF Research Database (Denmark)

    Pedersen, Claus Valling

    2007-01-01

    Anmeldelse af et overvejende filologisk mindeskrift (monografi) til ære for D.N. MacKenzie indeholdende artikler om iranske sprog......Anmeldelse af et overvejende filologisk mindeskrift (monografi) til ære for D.N. MacKenzie indeholdende artikler om iranske sprog...

  3. Plasma renin and prorenin and renin gene variation in patients with insulin-dependent diabetes mellitus and nephropathy

    NARCIS (Netherlands)

    J. Deinum (Jacob); L. Tarnow; J.M. van Gool (Jeanette); R.J.A. de Bruin (René); F.H.M. Derkx (Frans); M.A.D.H. Schalekamp (Maarten); H.H. Parving

    1999-01-01

    textabstractBackground. The most striking abnormality in the renin-angiotensin system in diabetic nephropathy (DN) is increased plasma prorenin. Renin is thought to be low or normal in DN. In spite of altered (pro)renin regulation the renin gene has not been studied for contribution to the

  4. Heparanase is essential for the development of diabetic nephropathy in mice.

    NARCIS (Netherlands)

    Gil, N.; Goldberg, R.; Neuman, T.; Garsen, M.; Zcharia, E.; Rubinstein, A.M.; Kuppevelt, T. van; Meirovitz, A.; Pisano, C.; Li, J.P.; Vlag, J. van der; Vlodavsky, I.; Elkin, M.

    2012-01-01

    Diabetic nephropathy (DN) is the major life-threatening complication of diabetes. Abnormal permselectivity of glomerular basement membrane (GBM) plays an important role in DN pathogenesis. Heparanase is the predominant enzyme that degrades heparan sulfate (HS), the main polysaccharide of the GBM.

  5. Rapidity dependence of particle densities in pp and AA collisions

    CERN Document Server

    Bautista, Irais; Milhano, Jose Guilherme; Dias de Deus, Jorge

    2012-01-01

    We use multiple scattering and energy conservation arguments to describe $dn/d\\eta_{NANA}$ as a function of $dn/d\\eta_{pp}$ in the framework of string percolation. We discuss the pseudo-rapidity $\\eta$? and beam rapidity Y dependence of particle densities. We present our results for pp, Au- Au, and Pb-Pb collisions at RHIC and LHC.

  6. Dicty_cDB: Contig-U16338-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available a melanogaster mixed e... 42 6.6 1 ( FD376962 ) CBPN7982.g1 CBPN: Subtracted channel catfish cDNA... 42 6.6 ...1 ( FD366351 ) CBPN44922.g1 CBPN: Subtracted channel catfish cDN... 42 6.6 1 ( FD350730 ) CBPN29177.g1 CBPN: Subtracted channel catfi...sh cDN... 42 6.6 1 ( FD324692 ) CBPN12039.g1 CBPN: Subtracted channel catfish... cDN... 42 6.6 1 ( FD188705 ) CBZA20575.g1 CBZA: Normalized channel catfish cDN... 42 6....6 1 ( FD187385 ) CBZA19839.g1 CBZA: Normalized channel catfish cDN... 42 6.6 1 ( FD177926 ) CBZA14364.g1 CB

  7. Dicty_cDB: Contig-U03338-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 3 ( AV929262 ) Hordeum vulgare subsp. vulgare cv.Haruna Nijo cDN... 280 e-113 3 ...J298309 ) Triticum aestivum cDNA clone:whsl24e04, 3' end, s... 272 6e-99 2 ( AV920301 ) Hordeum vulgare subsp. vulgare cv.Haruna... Nijo cDN... 280 2e-95 2 ( AV928609 ) Hordeum vulgare subsp. vulgare cv.Haruna Nijo cDN....um vulgare subsp. vulgare cv.Haruna Nijo cDN... 280 4e-94 2 ( BJ476030 ) Hordeum vulgare subsp. vulgare cv.Haruna... Nijo cDN... 280 4e-94 2 ( BJ465796 ) Hordeum vulgare subsp. vulgare cv.Haruna

  8. Clinical Proteomics: The Potentiality of Urine Analysis for Understanding Diabetic Nephropathy

    Directory of Open Access Journals (Sweden)

    Massimo Paple

    2013-07-01

    Full Text Available The incidence of diabetic nephropathy (DN is constantly rising in parallel with the prevalence of type 2 diabetes and has been predicted to double within the next 15 years. Albuminuria is considered the earliest putative diagnostic sign of diabetic renal damage but it is poorly associated to the complex histopathological picture of glomerular and tubular damage hence, up to now, the accurate diagnosis of the DN requires renal biopsy. The identification of new biomarkers of DN is an urgent need since the proper management of the DN patients requires early and unbiased diagnosis. The Proteomics approach to the study of the human disease allows a large-scale characterisation of the protein content of a biological sample, and its application to urine may be a challenging but powerful strategy to identify new DN biomarkers. In this review we discuss the main results of a decade of proteomic studies focused on the urinary investigation of diabetic nephropathy.

  9. Stochastic Resonance in a Bistable System Subject to Dichotomous Noise

    Institute of Scientific and Technical Information of China (English)

    ZHOU Yu-Rong; PAN Hui-Mei; GUO Feng; PANG Xiao-Feng

    2008-01-01

    The stochastic resonance phenomenon in a bistable system subject to Markov dichotomous noise (DN) is investigated. Based on the adiabatic elimination and the two-state theories, the explicit expressions for the signal-to-noise ratio (SNR) and the spectral power amplification (SPA) have been obtained. It is shown that two peaks can occur on the curve of SNR versus the intensity of the DN. Moreover, the SNR is a non-monotonic function of the correlation time of the DN. The SPA varies non-monotonously with the strength of the DN. The dependence of the SNR on the frequency and the amplitude of the external periodic signal are discussed. The effect of the external frequency and the correlation time of the DN on the SPA are analyzed.

  10. Assessment of cardiac sympathetic nerve abnormalities by {sup 123}I-MIBG (metaiodobenzylguanidine) myocardial scintigraphy in diabetic patients undergoing hemodialysis

    Energy Technology Data Exchange (ETDEWEB)

    Ohashi, Hiroshige; Oda, Hiroshi; Matsuno, Yukihiko [Prefectural Gifu Hospital (Japan)] [and others

    1995-05-01

    We compared cardiac sympathetic nerve abnormalities in patients hemodialyzed because of diabetic nephropathy (DN, n=18) and chronic glomerulonephritis (CGN, n=21). {sup 123}I-metaiodobenzylguanidine (MIBG) myocardial scintigraphy was performed in this study. SPECT and anterior planar myocardial images were obtained 15 minutes after (initial images) and 4 hours after (delayed images) an injection of MIBG. The following results were obtained: (1) SPECT showed more defects in DN than in CGN. (2) The heart to superior mediastinum uptake ratio (H/M) was lower in DN than in CGN. These findings suggest that myocardial uptake of MIBG in DN is significantly impaired because of cardiac sympathetic nerve abnormalities. These abnormalities may affect the prognosis in DN. (author).

  11. Mouse Models for Studying Diabetic Nephropathy.

    Science.gov (United States)

    Chow, Bryna S M; Allen, Terri J

    2015-06-01

    Diabetic nephropathy (DN) is a term used to describe kidney damage cause by diabetes. With DN as one of the leading causes of end-stage renal disease worldwide, there is a strong need for appropriate animal models to study DN pathogenesis and develop therapeutic strategies. To date, most experiments are carried out in mouse models as opposed to other species for several reasons including lower cost, ease of handling, and easy manipulation of the mouse genome to generate transgenic and knockout animals. This unit provides detailed insights and technical knowledge in setting up one of the most widely used models of DN, the streptozotocin (STZ)-induced model. This model has been extensively exploited to study the mechanism of diabetic renal injury. The advantages and limitations of the STZ model and the availability of other genetic models of DN are also discussed.

  12. Determination of the cathode layer thickness in the normal glow discharge

    Science.gov (United States)

    Hou, Xinyu; Fu, Yangyang; Wang, Hao; Zou, Xiaobing; Luo, Haiyun; Wang, Xinxin

    2017-08-01

    Two methods for the determination of the cathode layer thickness dn in the normal glow discharge were developed. The first one is the computational method based on the iteration with a differently assumed value of dn. The second one is the experimental method with a Langmuir probe. The computational results showed that the reduced cathode layer thickness p.dn monotonically decreases and finally saturates with the increase in the cathode fall. It was found with these two methods that p.dn is a constant for the given cathode fall and secondary electron emission coefficient. This implies that the cathode layer will automatically adjust its thickness to keep p.dn a constant when the gas pressure changes. The results obtained with these two developed methods were compared with the results obtained with the numerical simulation of the normal glow discharge, which shows a good agreement.

  13. Distribution of neuropeptide Y Leu7Pro polymorphism in patients with type 1 diabetes and diabetic nephropathy among Swedish and American populations.

    Science.gov (United States)

    Ma, Jun; Nordman, Sofia; Möllsten, Anna; Falhammar, Henrik; Brismar, Kerstin; Dahlquist, Gisela; Efendic, Suad; Gu, Harvest F

    2007-11-01

    The distribution of Leu7Pro polymorphism in the neuropeptide Y gene shows a geographical north to south gradient of decreasing frequency, suggesting that it may be a population-specific causal variant. This polymorphism is found to be associated with diabetic nephropathy (DN) and coronary heart disease in Finnish women with type 1 diabetes (T1D). The present study aims to evaluate the susceptibility of this polymorphism to the development of DN in two different populations. One sample set consists of 174 (females 98 and males 76) Swedish T1D patients with DN and 249 (females 132 and males 117) patients without DN. Another sample set includes 597 (females 356 and males 241) American T1D patients without DN and 577 (females 264 and males 313) patients with DN, who were descents of European Caucasians and were from the Genetics of Kidneys in Diabetes (GoKinD) Study. Genotyping of Leu7Pro polymorphism was performed by dynamic allele-specific hybridization. The C allele frequencies of Leu7Pro polymorphism in T1D patients between Swedish and American GoKinD populations were significantly different (6.3 vs 4.0%; P=0.006). Particularly, the C allele frequency in Swedish female T1D patients with DN was significantly higher in comparison with T1D patients without DN (10.2 vs 4.2%; P=0.011, OR=2.614, 95% confidence intervals: 1.249-5.467). No significant association of this polymorphism with DN was observed in Swedish male T1D patients and the patients from GoKinD. The present study provides further evidence that Leu7Pro polymorphism confers the susceptibility to the development of DN in Swedish female T1D patients.

  14. Evidence and expert opinions: Dry needling versus acupuncture (I) : The American Alliance for Professional Acupuncture Safety (AAPAS) White Paper 2016.

    Science.gov (United States)

    Fan, Arthur Yin; Xu, Jun; Li, Yong-Ming

    2017-01-01

    In the last twenty years, in the United States and other Western countries, dry needling (DN) became a hot and debatable topic, not only in academic but also in legal fields. This White Paper is to provide the authoritative information of DN versus acupuncture to academic scholars, healthcare professional administrators, lawmakers, and the general public through providing the authoritative evidence and experts' opinions regarding critical issues of DN versus acupuncture, and then reach consensus. DN is the use of dry needles alone, either solid filiform acupuncture needles or hollow-core hypodermic needles, to insert into the body for the treatment of muscle pain and related myofascial pain syndrome. DN is sometimes also known as intramuscular stimulati on, trigger points (TrP) acupuncture, TrP DN, myofascial TrP DN, or biomedical acupuncture. In Western countries, DN is a form of simplified acupuncture using biomedical language in treating myofascial pain, a contemporary development of a portion of Ashi point acupuncture from Chinese acupuncture. It seeks to redefine acupuncture by reframing its theoretical principles in a Western manner. DN-like needling with filiform needles have been widely used in Chinese acupuncture practice over the past 2,000 years, and with hypodermic needles has been used in China in acupuncture practice for at least 72 years. In Eastern countries, such as China, since late of 1800s or earlier, DN is a common name of acupuncture among acupuncturists and the general public, which has a broader scope of indications, not limited to treating the myofascial pain.

  15. New susceptibility loci associated with kidney disease in type 1 diabetes.

    Directory of Open Access Journals (Sweden)

    Niina Sandholm

    2012-09-01

    Full Text Available Diabetic kidney disease, or diabetic nephropathy (DN, is a major complication of diabetes and the leading cause of end-stage renal disease (ESRD that requires dialysis treatment or kidney transplantation. In addition to the decrease in the quality of life, DN accounts for a large proportion of the excess mortality associated with type 1 diabetes (T1D. Whereas the degree of glycemia plays a pivotal role in DN, a subset of individuals with poorly controlled T1D do not develop DN. Furthermore, strong familial aggregation supports genetic susceptibility to DN. However, the genes and the molecular mechanisms behind the disease remain poorly understood, and current therapeutic strategies rarely result in reversal of DN. In the GEnetics of Nephropathy: an International Effort (GENIE consortium, we have undertaken a meta-analysis of genome-wide association studies (GWAS of T1D DN comprising ~2.4 million single nucleotide polymorphisms (SNPs imputed in 6,691 individuals. After additional genotyping of 41 top ranked SNPs representing 24 independent signals in 5,873 individuals, combined meta-analysis revealed association of two SNPs with ESRD: rs7583877 in the AFF3 gene (P = 1.2 × 10(-8 and an intergenic SNP on chromosome 15q26 between the genes RGMA and MCTP2, rs12437854 (P = 2.0 × 10(-9. Functional data suggest that AFF3 influences renal tubule fibrosis via the transforming growth factor-beta (TGF-β1 pathway. The strongest association with DN as a primary phenotype was seen for an intronic SNP in the ERBB4 gene (rs7588550, P = 2.1 × 10(-7, a gene with type 2 diabetes DN differential expression and in the same intron as a variant with cis-eQTL expression of ERBB4. All these detected associations represent new signals in the pathogenesis of DN.

  16. Effects of MCF2L2, ADIPOQ and SOX2 genetic polymorphisms on the development of nephropathy in type 1 Diabetes Mellitus

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    Gu Harvest F

    2010-07-01

    Full Text Available Abstract Background MCF2L2, ADIPOQ and SOX2 genes are located in chromosome 3q26-27, which is linked to diabetic nephropathy (DN. ADIPOQ and SOX2 genetic polymorphisms are found to be associated with DN. In the present study, we first investigated the association between MCF2L2 and DN, and then evaluated effects of these three genes on the development of DN. Methods A total of 1177 type 1 diabetes patients with and without DN from the GoKinD study were genotyped with TaqMan allelic discrimination. All subjects were of European descent. Results Leu359Ile T/G variant in the MCF2L2 gene was found to be associated with DN in female subjects (P = 0.017, OR = 0.701, 95%CI 0.524-0.938 but not in males. The GG genotype carriers among female patients with DN had tendency decreased creatinine and cystatin levels compared to the carriers with either TT or TG genotypes. This polymorphism MCF2L2-rs7639705 together with SNPs of ADIPOQ-rs266729 and SOX2-rs11915160 had combined effects on decreased risk of DN in females (P = 0.001. Conclusion The present study provides evidence that MCF2L2, ADIPOQ and SOX2 genetic polymorphisms have effects on the resistance of DN in female T1D patients, and suggests that the linkage with DN in chromosome 3q may be explained by the cumulated genetic effects.

  17. Single-Dose Pharmacokinetic Study of Tramadol Extended-Release Tablets in Children and Adolescents.

    Science.gov (United States)

    Vandenbossche, Joris; Van Peer, Achiel; Richards, Henry

    2016-09-01

    Combined analyses from 2 open-label, phase-1 studies-the pharmacokinetic profile of tramadol and its metabolite (M1) following a single oral dose of tramadol extended release (ER) (25 to 100 mg) in children (7 to 11 years old; study 1: n = 37) and adolescents (12 to 17 years old; study 2: n = 38) with painful conditions-were historically compared with that of healthy adults following similar dosing. The dose-normalized area under the curve (DN AUC0-24h ) and maximum concentration (DN Cmax ) of tramadol and of M1 in children and in adolescents were lower than those in adults (children vs adults: tramadol, DN AUC0-24h 82.19%; DN Cmax 80.38%, P = .0031; M1, DN AUC0-24h 51.19%, DN Cmax 52.68%, P < .0001; adolescents vs adults: tramadol, DN AUC0-24h 89.56%, DN Cmax 84.01%; M1, DN AUC0-24h 85.28%, DN Cmax 83.03%, P = .0004). The arithmetic mean terminal elimination t1/2 of tramadol in children and adolescents was comparable to that in adults (children 8.4 hours; adolescents 8.5 hours; adults 7.9 hours). The most frequently reported (≥5% of participants) treatment-emergent adverse events in children included headache, upper abdominal pain and constipation, and in adolescents were headache, nausea, dizziness, and stomach discomfort. Multiple factors may have contributed to these observations, including a higher proportion of children (56%) who may have a lower activity of CYP2D6, resulting in reduced clearance of tramadol.

  18. New susceptibility loci associated with kidney disease in type 1 diabetes.

    Directory of Open Access Journals (Sweden)

    Niina Sandholm

    2012-09-01

    Full Text Available Diabetic kidney disease, or diabetic nephropathy (DN, is a major complication of diabetes and the leading cause of end-stage renal disease (ESRD that requires dialysis treatment or kidney transplantation. In addition to the decrease in the quality of life, DN accounts for a large proportion of the excess mortality associated with type 1 diabetes (T1D. Whereas the degree of glycemia plays a pivotal role in DN, a subset of individuals with poorly controlled T1D do not develop DN. Furthermore, strong familial aggregation supports genetic susceptibility to DN. However, the genes and the molecular mechanisms behind the disease remain poorly understood, and current therapeutic strategies rarely result in reversal of DN. In the GEnetics of Nephropathy: an International Effort (GENIE consortium, we have undertaken a meta-analysis of genome-wide association studies (GWAS of T1D DN comprising ~2.4 million single nucleotide polymorphisms (SNPs imputed in 6,691 individuals. After additional genotyping of 41 top ranked SNPs representing 24 independent signals in 5,873 individuals, combined meta-analysis revealed association of two SNPs with ESRD: rs7583877 in the AFF3 gene (P = 1.2 × 10(-8 and an intergenic SNP on chromosome 15q26 between the genes RGMA and MCTP2, rs12437854 (P = 2.0 × 10(-9. Functional data suggest that AFF3 influences renal tubule fibrosis via the transforming growth factor-beta (TGF-β1 pathway. The strongest association with DN as a primary phenotype was seen for an intronic SNP in the ERBB4 gene (rs7588550, P = 2.1 × 10(-7, a gene with type 2 diabetes DN differential expression and in the same intron as a variant with cis-eQTL expression of ERBB4. All these detected associations represent new signals in the pathogenesis of DN.

  19. Low bone mineral density is associated with bone microdamage accumulation in postmenopausal women with osteoporosis.

    Science.gov (United States)

    Stepan, Jan J; Burr, David B; Pavo, Imre; Sipos, Adrien; Michalska, Dana; Li, Jiliang; Fahrleitner-Pammer, Astrid; Petto, Helmut; Westmore, Michael; Michalsky, David; Sato, Masahiko; Dobnig, Harald

    2007-09-01

    Marked suppression of bone turnover by bisphosphonates is associated with increased bone microdamage accumulation in animal models. The purpose of this study was to test the hypothesis that long-term treatment with alendronate (ALN) results in accumulation of microdamage in bone in women after menopause. Sixty-six postmenopausal women with osteoporosis (mean age of 68.0 years and mean BMD T-score of -1.7 at total hip and -2.8 at lumbar spine; 62% with prevalent fractures) were evaluated in this cross-sectional analysis. Thirty-eight had been treated previously with ALN (10 mg/day or 70 mg/week for a mean duration of 63.6 months) while twenty-eight were treatment naive (TN). Without adjustments, crack surface density (Cr.S.Dn) and crack density (Cr.Dn) were not different between ALN and TN patients. After adjustment for potential confounders (age, prevalent fractures, femoral neck BMD, activation frequency and center), Cr.Dn was elevated in ALN patients (P=0.028 and P=0.069 for Cr.S.Dn). In ALN patients only, lower femoral neck BMD (Cr.S.Dn, r=-0.58, P=0.003; Cr.Dn, r=-0.54, P=0.005) and increased age (Cr.S.Dn, r=0.43, P=0.03; Cr.Dn, r=0.43, P=0.03) were associated with microdamage accumulation. Among potential confounders, femoral neck BMD was the only independent predictor for these correlations (P=0.04 for Cr.Dn and P=0.03 for Cr.S.Dn). We conclude that increased microdamage accumulation may occur in low BMD patients treated with alendronate.

  20. Simulation modeling for nitrogen removal and experimental estimation of mass fractions of microbial groups in single-sludge system.

    Science.gov (United States)

    Huang, J S; Tsai, C C; Chou, H H; Ting, W H

    2006-01-01

    Nitrification-denitrification in a single-sludge nitrogen removal system (SSNRS; with a sufficient carbon source for denitrification) was performed. With an increase in the mixed liquor recycle ratio (R(m)) from 1 to 2, the total nitrogen (TN) removal efficiency at a lower volumetric loading rate (VLR=0.21 NH(4)(+)-N m(-3) d(-1)) increased, but the TN removal efficiency at a higher VLR (0.35 kg NH(4)(+)-N m(-3) d(-1)) decreased. A kinetic model that accounts for the mass fractions of Nitrosomonas, Nitrobacter, nitrate reducer and nitrite reducer (f(n1), f(n2), f(dn1), and f(dn2)) in the SSNRS and an experimental approach for the estimation of the mass fractions of nitrogen-related microbial groups are also proposed. The estimated f(dn1) plus f(dn2) (0.65-0.83) was significantly larger than the f(n1) plus f(n2) (0.28-0.32); the f(n1) (0.21-0.26) was larger than the f(n2) (0.05-0.07); and the f(dn1) (0.32-0.45) varied slightly with the f(dn2) (0.33-0.38). At the lower VLR, the f(dn1) plus f(dn2) increased with increasing R(m); however at the higher VLR, the f(dn1) plus f(dn2) did not increase with increasing R(m). By using the kinetic model, the calculated residual NH(4)(+)-N and NO(2)(-)-N in the anoxic reactor and NO(2)(-)-N and NO(3)(-)-N in the aerobic reactor were in fairly good agreement with the experimental data; the calculated NO(3)(-)-N in the anoxic reactor was over-estimated and the calculated NH(4)(+)-N in the aerobic reactor was under-estimated.

  1. New Susceptibility Loci Associated with Kidney Disease in Type 1 Diabetes

    Science.gov (United States)

    Forsblom, Carol; Isakova, Tamara; McKay, Gareth J.; Williams, Winfred W.; Sadlier, Denise M.; Mäkinen, Ville-Petteri; Swan, Elizabeth J.; Palmer, Cameron; Boright, Andrew P.; Ahlqvist, Emma; Deshmukh, Harshal A.; Keller, Benjamin J.; Huang, Huateng; Ahola, Aila J.; Fagerholm, Emma; Gordin, Daniel; Harjutsalo, Valma; He, Bing; Heikkilä, Outi; Hietala, Kustaa; Kytö, Janne; Lahermo, Päivi; Lehto, Markku; Lithovius, Raija; Österholm, Anne-May; Parkkonen, Maija; Pitkäniemi, Janne; Rosengård-Bärlund, Milla; Saraheimo, Markku; Sarti, Cinzia; Söderlund, Jenny; Soro-Paavonen, Aino; Syreeni, Anna; Thorn, Lena M.; Tikkanen, Heikki; Tolonen, Nina; Tryggvason, Karl; Tuomilehto, Jaakko; Wadén, Johan; Gill, Geoffrey V.; Prior, Sarah; Guiducci, Candace; Mirel, Daniel B.; Taylor, Andrew; Hosseini, S. Mohsen; Parving, Hans-Henrik; Rossing, Peter; Tarnow, Lise; Ladenvall, Claes; Alhenc-Gelas, François; Lefebvre, Pierre; Rigalleau, Vincent; Roussel, Ronan; Tregouet, David-Alexandre; Maestroni, Anna; Maestroni, Silvia; Falhammar, Henrik; Gu, Tianwei; Möllsten, Anna; Cimponeriu, Danut; Ioana, Mihai; Mota, Maria; Mota, Eugen; Serafinceanu, Cristian; Stavarachi, Monica; Hanson, Robert L.; Nelson, Robert G.; Kretzler, Matthias; Colhoun, Helen M.; Panduru, Nicolae Mircea; Gu, Harvest F.; Brismar, Kerstin; Zerbini, Gianpaolo; Hadjadj, Samy; Marre, Michel; Groop, Leif; Lajer, Maria; Bull, Shelley B.; Waggott, Daryl; Paterson, Andrew D.; Savage, David A.; Bain, Stephen C.; Martin, Finian; Hirschhorn, Joel N.; Godson, Catherine; Florez, Jose C.; Groop, Per-Henrik; Maxwell, Alexander P.

    2012-01-01

    Diabetic kidney disease, or diabetic nephropathy (DN), is a major complication of diabetes and the leading cause of end-stage renal disease (ESRD) that requires dialysis treatment or kidney transplantation. In addition to the decrease in the quality of life, DN accounts for a large proportion of the excess mortality associated with type 1 diabetes (T1D). Whereas the degree of glycemia plays a pivotal role in DN, a subset of individuals with poorly controlled T1D do not develop DN. Furthermore, strong familial aggregation supports genetic susceptibility to DN. However, the genes and the molecular mechanisms behind the disease remain poorly understood, and current therapeutic strategies rarely result in reversal of DN. In the GEnetics of Nephropathy: an International Effort (GENIE) consortium, we have undertaken a meta-analysis of genome-wide association studies (GWAS) of T1D DN comprising ∼2.4 million single nucleotide polymorphisms (SNPs) imputed in 6,691 individuals. After additional genotyping of 41 top ranked SNPs representing 24 independent signals in 5,873 individuals, combined meta-analysis revealed association of two SNPs with ESRD: rs7583877 in the AFF3 gene (P = 1.2×10−8) and an intergenic SNP on chromosome 15q26 between the genes RGMA and MCTP2, rs12437854 (P = 2.0×10−9). Functional data suggest that AFF3 influences renal tubule fibrosis via the transforming growth factor-beta (TGF-β1) pathway. The strongest association with DN as a primary phenotype was seen for an intronic SNP in the ERBB4 gene (rs7588550, P = 2.1×10−7), a gene with type 2 diabetes DN differential expression and in the same intron as a variant with cis-eQTL expression of ERBB4. All these detected associations represent new signals in the pathogenesis of DN. PMID:23028342

  2. Leishmaniasis visceral en Medula Ósea

    OpenAIRE

    Méndez López, Max A.; López Villegas, Jorge

    2016-01-01

    Una mujer de 47 años, fue evaluada en Emergencias con fiebre y pancitopenia, con velocidad de eritrosedimentación en 160mm/h (0-20mm/h) y deshidrogenasa láctica en 980 U/L (140-280 U/L). Había sido diagnosticada VIH positiva desde hace 7 años y se mantenía con terapia antiretroviral hasta 6 meses atrás cuando decidió abandonar el seguimiento y medicación. A su llegada, el ultrasonido de abdomen documentó hepatoesplenomegalia sin adenopatías. Se puede apreciar en las imágenes un histiocito fag...

  3. EFECTO DE DIFERENTES INOCULANTES SOBRE LA ACTIVIDAD MICROBIANA EN LA RIZÓSFERA DEL CULTIVO DE PALLAR (Phaseolus lunatus var. sieva) EN CONDICIONES DE CAMPO

    OpenAIRE

    Ramos Vásquez, Elena; Universidad Nacional Agraria La Molina (Perú).; Zúñiga Dávila, Doris; Universidad Nacional Agraria La Molina (Perú).

    2008-01-01

    Se evaluó la actividad microbiana en la rizósfera de Phaseolus lunatus var. sieva, a partir de muestras de suelo colectadas en campo durante los diferentes estados fenológicos del cultivo, cuyas semillas fueron inoculadas con Bradyrhizobium sp. cepa PCYGIVN3, cepa PSNC4N2 y la interacción de éstas; además, se evaluó la aplicación de fertilizante nitrogenado N-P-K y el control sin inocular. Se determinaron la respiración y la actividad deshidrogenasa de los microorganismos en la rizósfera, las...

  4. Modelo teórico para explicar la acumulación de gotas lipídicas en embriones bovinos machos o hembras producidos in vitro

    OpenAIRE

    OLIVERA MARTHA; CAMARGO OMAR; RUIZ TATIANA

    2008-01-01

    La glucosa 6-fosfato deshidrogenasa (G6PD), codificada por un gen ubicado en el cromosoma X, es la enzima limitante de vía de las pentosas fosfato (PF). La entrada de la glucosa así como su flujo y el rendimiento metabólico de esta vía están determinados tanto por los mismos niveles glucosa así como por la actividad de la G6PD. Por esta vía, la glucosa regula la trascripción de varios genes lipogénicos. En algunos embriones hembra producidos in vitro, se regis...

  5. Effect of short-term ornithine alpha-ketoglutarate pretreatment on intestinal ischemia-reperfusion in rats Efeitos do pré-tratamento em curto prazo com ornitina alfa-cetoglutarato na isquemia-reperfusão intestinal em ratos

    Directory of Open Access Journals (Sweden)

    Eduardo Silvio Gouveia Gonçalves

    2011-01-01

    grupo G5 foi submetido à reperfusão por 30 minutos. Amostras de sangue foram coletadas no final da laparotomia (G1, após 30 minutos (G2, G4 e 60 minutos (G3, G5 para determinação das concentrações de metabolitos (piruvato, lactato, creatinofosfoquinase (CPK, substâncias reativas ao ácido tiobarbitúrico (TBARS e glutationa (GSH. RESULTADOS: Observou-se redução significante (p<0,05 das concentrações de piruvato e lactato, teciduais e CPK plasmático em ratos tratados com OKG, no final do período de reperfusão. Não houve alteração significante nos níveis plasmáticos e teciduais de GSH. Entretanto os níveis de TBARS aumentaram significativamente (p<0,05 em amostras de tecido de ratos tratados com OKG submetido à isquemia por 30 minutos. CONCLUSÃO: o pré-tratamento em curto prazo com OKG antes da indução da I/R diminui a lesão tecidual, aumenta a utilização de piruvato para produção de energia no ciclo de Krebs, mas não atenua o estresse oxidativo neste modelo animal.

  6. Producción de 1,4-androstadien-3,17-diona a partir de colesterol por Mycobacterium sp., empleando azul de metileno como aceptor electrónico

    Directory of Open Access Journals (Sweden)

    Alina Falero

    2004-01-01

    Full Text Available La formación de AD y ADD a partir de colesterol en microorganismos involucra la participación de diferentes enzimas. Cada una de ellas tiene sus propios requerimientos específicos. El primer paso en la degradaci ón del colesterol es la oxidación del grupo OH que está situado en posici ón 3 del anillo A de la estructura química. Posteriormente ocurre una isomerización del doble enlace en posición 5 del anillo B a posición 4 del anillo A, para formar el 3-ceto-D4-esteroide correspondiente. En la oxidaci ón intervienen deshidrogenasas NAD+ dependientes y oxidasas, siendo el oxígeno molecular el aceptor final de la reacción. Se ha demostrado que la insaturación del esteroide ocurre mediante la eliminación de los hidrógenos 1 y 2 del anillo A. Este paso está mediado por la enzima esteroide-1,2- deshidrogenasa. Esta enzima es inducible y se encuentra en bacterias asociada a membrana. Para su funcionamiento normalmente necesita de aceptores electrónicos externos. Para estos fines, se han utilizado aceptores electrónicos artificiales tales como: menadiona, metasulfato de fenacina, 2,6-diclorofenolindofenol y azul de metileno. En este trabajo se aportan los resultados de la utilización del azul de metileno en la cepa NRRL B-3683 Mycobacterium sp. Si el aceptor electrónico se añade al inicio de la reacci ón, la biotransformación disminuye drásticamente. Sin embargo, se observa un incremento notable en la cantidad de ADD formado, si el azul de metileno es añadido a las 72 h de iniciada la reacción.

  7. POLIMORFISMO BIOQUÍMICO EN CULTIVARES DE FRESA (Fragaria ananassa DUCH.

    Directory of Open Access Journals (Sweden)

    Argelys Kessel Dominí

    2014-01-01

    Full Text Available La caracterización genética de los cultivares de fresa (Fragaria ananassa Duch. existentes en Cuba resulta de gran interés ya que permite obtener información básica necesaria para el diseño de estrategias para la conservación de este germoplasma y para futuros programas de mejora del cultivo en el país, con el fin de seleccionar cultivares mejor adaptados a nuestras condiciones edafoclimáticas. El presente trabajo tuvo como objetivo determinar la variabilidad, en siete genotipos de fresa cultivados en diferentes localidades de la isla mediante el empleo de marcadores bioquímicos. El análisis se realizó para los sistemas isoenzimáticos esterasas, anhidrasa carbónica, fosfatasas ácidas y malato deshidrogenasa. El grado de polimorfismo total para los sistemas estudiados fue elevado, de un 89 %, siendo el sistema malato deshidrogenasa el más polimórfico con un 100 %. El mayor nivel de polimorfismo, entre cultivares, se observó en el cultivar ‘Misionaria’ con un 81,2 % y el menor en el cultivar de Villa Clara con un 32,4 %. Se construyó una matriz de presencia-aunsencia de bandas, la que se procesó por el paquete del programa estadístico Past versión 2,14; se empleó el índice de Jaccard y el método UPGMA para obtener el dendograma. Estos resultados permitieron conocer el grado de variabilidad presente en los cultivares de fresa existentes en Cuba, información que podrá ser aprovechada por los programas de mejoramiento genético del cultivo.

  8. Caracterização da mitocôndria isolada de fígado de tilápia-do-nilo (Oreochromis niloticus e alterações da bioenergética mitocondrial causadas pela exposição herbicida oxifluorfena Characterization of liver mitochondria from Nile tilapia (Oreochromis niloticus and mitochondrial bioenergetics alterations caused by exposure to oxyfluorfen herbicide

    Directory of Open Access Journals (Sweden)

    F.P. Peixoto

    2009-04-01

    Full Text Available Descreve-se um método de isolamento de mitocôndrias acopladas de tilápia-do-nilo Oreochromis niloticus, isoladas de células hepáticas de peixes adultos. As mitocôndrias estavam metabolicamente ativas, sendo capazes de realizarem fosforilação oxidativa, de acordo com os valores do quociente de controle respiratório. Os valores de controle respiratório obtidos com malato/piruvato (complexo I e com succinato (complexo II foram de 5,8±0,8 e 3,38±0,4, respectivamente. O potencial de membrana exibiu o valor de 197±4mV, quer se utilizasse malato/piruvato ou succinato como substrato. O procedimento de isolamento de mitocôndrias de O. niloticus permite o estudo do efeito de xenobióticos na bioenergética mitocondrial, tendo sido avaliada a ação da oxifluorfena (0,6mgL-1 na bioenergética mitocondrial. Os resultados demonstram que o tratamento com oxifluorfena influencia a capacidade fosforilativa dos peixes, interferindo na sua carga energética, o que poderá levar à sua morte.A method for isolation of coupled mitochondria isolated from the liver of adult Nile tilapia Oreochromis niloticus is described for the first time. They were metabolically active, able to sustain oxidative phosphorylation, as shown by respiratory control ratio values, which were about 5.8±0.8 and 3.3±0.4 when respiring on malate/piruvate (complex I or succinate (complex II, respectively, as substrate. Membrane potential exhibited a value of approximately 197±4mV for malate/piruvate or succinate. The procedure now described for the isolation of O. niloticus mitochondria is an important new tool, allowing the study about the effect of xenobiotics on mitochondrial bioenergetic, being evaluated the effect of oxyfluorfen (0.6mgL-1 in the liver mitocondrial bioenergetic. These results showed that phosphorylation was significantly affected by oxyfluorfen which contributed to the decrease on the liver cell energy charge and consequently led to the fish dead.

  9. EFECTO DE NIVELES CRECIENTES DE NITRÓGENO NO PROTÉICO DIETARIO SOBRE LA CONCENTRACIÓN DE PRECURSORES GLUCONEOGÉNICOS EN HÍGADO BOVINO EFFECT OF INCREASING LEVELS OF DIETARY NONPROTEIN NITROGEN ON GLUCONEOGENIC PRECURSORS CONCENTRATION IN BOVINE LIVER

    Directory of Open Access Journals (Sweden)

    Rubén Darío Galvis Goez

    2010-06-01

    Full Text Available Con el fin de evaluar el efecto de niveles crecientes de proteína degradable en rumen (PDR sobre las concentraciones de amonio y piruvato en hígado, se utilizaron 15 vacas Holstein que cursaban los primeros cien días posparto. Estas se asignaron aleatoriamente a tres tratamientos experimentales que consistieron en el suministro de un suplemento alimenticio comercial al que se le adicionó urea de tal manera que se incrementará el consumo de PDR en aproximadamente 0 (T0, 10 (T1 y 15% (T2 respecto a los requerimientos. A estas vacas se les determinaron las concentraciones hepáticas de amonio (Ahep y piruvato (Pihep. Se realizó un balance nutricional de energía neta de lactancia (ENL, proteína metabolizable (PM, PDR y proteína no degradable en rumen (PNDR donde se encontraron diferencias significativas entre los tratamientos (PIn order to test the effect of growing levels of diet rumen degradable protein on hepatic concentrations of ammonia and piruvate, fifteen cows Holstein in their first one hundred post calving days were used. They were assigned at random to three different experimental treatments, which consisted on the supply of a commercial feeding supplement without any addition of urea as a source of NNP (T0; the second treatment (T1 consisted on the supply of the same supplement with an addition of urea, so that the supply of PDR was increased 10% approximately to the requirements;finally, in the third treatment, (T2 the cows were given the same supplement which was added with enough urea so that the supply of PDR was increased 15% approximately to the requirements. In these cows were determined hepatic concentrations of ammonia (Ahep and pyruvate (Pyhep. The concentrations of Ahep were different among treatments, whereas the concentrations of Pihep were similar. Negative relations between Ahep and ENL, Ahep and PNDR, Ahep, and PM, were found it; while Pihep was positively related with Ahep. These results suggest the

  10. Improving Protein Fold Recognition by Deep Learning Networks

    Science.gov (United States)

    Jo, Taeho; Hou, Jie; Eickholt, Jesse; Cheng, Jianlin

    2015-12-01

    For accurate recognition of protein folds, a deep learning network method (DN-Fold) was developed to predict if a given query-template protein pair belongs to the same structural fold. The input used stemmed from the protein sequence and structural features extracted from the protein pair. We evaluated the performance of DN-Fold along with 18 different methods on Lindahl’s benchmark dataset and on a large benchmark set extracted from SCOP 1.75 consisting of about one million protein pairs, at three different levels of fold recognition (i.e., protein family, superfamily, and fold) depending on the evolutionary distance between protein sequences. The correct recognition rate of ensembled DN-Fold for Top 1 predictions is 84.5%, 61.5%, and 33.6% and for Top 5 is 91.2%, 76.5%, and 60.7% at family, superfamily, and fold levels, respectively. We also evaluated the performance of single DN-Fold (DN-FoldS), which showed the comparable results at the level of family and superfamily, compared to ensemble DN-Fold. Finally, we extended the binary classification problem of fold recognition to real-value regression task, which also show a promising performance. DN-Fold is freely available through a web server at http://iris.rnet.missouri.edu/dnfold.

  11. Personality and complex brain networks: The role of openness to experience in default network efficiency.

    Science.gov (United States)

    Beaty, Roger E; Kaufman, Scott Barry; Benedek, Mathias; Jung, Rex E; Kenett, Yoed N; Jauk, Emanuel; Neubauer, Aljoscha C; Silvia, Paul J

    2016-02-01

    The brain's default network (DN) has been a topic of considerable empirical interest. In fMRI research, DN activity is associated with spontaneous and self-generated cognition, such as mind-wandering, episodic memory retrieval, future thinking, mental simulation, theory of mind reasoning, and creative cognition. Despite large literatures on developmental and disease-related influences on the DN, surprisingly little is known about the factors that impact normal variation in DN functioning. Using structural equation modeling and graph theoretical analysis of resting-state fMRI data, we provide evidence that Openness to Experience-a normally distributed personality trait reflecting a tendency to engage in imaginative, creative, and abstract cognitive processes-underlies efficiency of information processing within the DN. Across two studies, Openness predicted the global efficiency of a functional network comprised of DN nodes and corresponding edges. In Study 2, Openness remained a robust predictor-even after controlling for intelligence, age, gender, and other personality variables-explaining 18% of the variance in DN functioning. These findings point to a biological basis of Openness to Experience, and suggest that normally distributed personality traits affect the intrinsic architecture of large-scale brain systems. Hum Brain Mapp 37:773-779, 2016. © 2015 Wiley Periodicals, Inc. © 2015 The Authors Human Brain Mapping Published by Wiley Periodicals, Inc.

  12. Long-term epidemiological study of disseminated neoplasia of cockles in Galicia (NW Spain): temporal patterns at individual and population levels, influence of environmental and cockle-based factors and lethality.

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    Díaz, S; Iglesias, D; Villalba, A; Carballal, M J

    2016-09-01

    The dynamics of disseminated neoplasia (DN) affecting cockles Cerastoderma edule (L.) in Galicia was addressed at individual and population levels. Early stage of DN was characterized by isolated neoplastic cells occurring in branchial vessels or in the connective tissue of gills, mantle, gonad or digestive gland. As disease progressed, the neoplastic cells appeared loose in foci and became widely distributed throughout the organs. In advanced stages, the connective tissue of most organs was infiltrated by neoplastic cells, which displaced normal cells, leading to the loss of the normal tissue/organ architecture. Host defence reaction was occasionally observed. A field survey performed for 7 years, in two cockle beds located in different Galician Rías, showed that DN is a hyperendemic disease usually present all year-round at high prevalence in adult cockles but with annual prevalence minima in spring likely due to the death of heavily affected cockles, concurrently with gonad ripeness-spawning. DN was detected in the cockles ranging from 10 to 39 mm in size; the highest DN prevalence and severity corresponded to the cockles of intermediate size/age (22-29 mm/0.7-1 year old). Sex did not appear to influence DN occurrence. An inhibitory effect of DN on cockle gametogenesis was detected.

  13. Different hydrogen isotope fractionations during lipid formation in higher plants: Implications for paleohydrology reconstruction at a global scale.

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    Liu, Jinzhao; Liu, Weiguo; An, Zhisheng; Yang, Hong

    2016-01-25

    Leaf wax δDn-alkane values have shown to differ significantly among plant life forms (e.g., among grasses, shrubs, and trees) in higher plants. However, the underlying causes for the differences in leaf wax δDn-alkane values among different plant life forms remain poorly understood. In this study, we observed that leaf wax δDn-alkane values between major high plant lineages (eudicots versus monocots) differed significantly under the same environmental conditions. Such a difference primarily inherited from different hydrogen biosynthetic fractionations (εwax-lw). Based upon a reanalysis of the available leaf wax δDn-alkane dataset from modern plants in the Northern Hemisphere, we discovered that the apparent hydrogen fractionation factor (εwax-p) between leaf wax δDn-alkane values of major angiosperm lineages and precipitation δD values exhibited distinguishable distribution patterns at a global scale, with an average of -140‰ for monocotyledonous species, -107‰ for dicotyledonous species. Additionally, variations of leaf wax δDn-alkane values and the εwax-p values in gymnosperms are similar to those of dicotyledonous species. Therefore, the data let us believe that biological factors inherited from plant taxonomies have a significant effect on controlling leaf wax δDn-alkane values in higher plants.

  14. Low dose EGCG treatment beginning in adolescence does not improve cognitive impairment in a Down syndrome mouse model.

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    Stringer, Megan; Abeysekera, Irushi; Dria, Karl J; Roper, Randall J; Goodlett, Charles R

    2015-11-01

    Down syndrome (DS) or Trisomy 21 causes intellectual disabilities in humans and the Ts65Dn DS mouse model is deficient in learning and memory tasks. DYRK1A is triplicated in DS and Ts65Dn mice. Ts65Dn mice were given up to ~20mg/kg/day epigallocatechin-3-gallate (EGCG), a Dyrk1a inhibitor, or water beginning on postnatal day 24 and continuing for three or seven weeks, and were tested on a series of behavioral and learning tasks, including a novel balance beam test. Ts65Dn as compared to control mice exhibited higher locomotor activity, impaired novel object recognition, impaired balance beam and decreased spatial learning and memory. Neither EGCG treatment improved performance of the Ts65Dn mice on these tasks. Ts65Dn mice had a non-significant increase in Dyrk1a activity in the hippocampus and cerebellum. Given the translational value of the Ts65Dn mouse model, further studies will be needed to identify the EGCG doses (and mechanisms) that may improve cognitive function.

  15. Detection of a novel population of fetal thymocytes characterized by preferential emigration and a TCRgammadelta+ T cell fate after dioxin exposure.

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    Majora, Marc; Frericks, Markus; Temchura, Vladimir; Reichmann, Gaby; Esser, Charlotte

    2005-11-01

    T cell maturation into TCRalphabeta(+) or TCRgammadelta(+) cells from common immature CD4(-)CD8(-)(DN) precursors occurs in the thymus, and is controlled through ordered regulation of genes. The aryl hydrocarbon receptor (AHR), a latent cytoplasmic transcription factor, affects thymocyte maturation and differentiation at several stages, also including DN cells. We analyzed in murine fetal thymus organ cultures (FTOC) the outcome of AHR-signaling and found a higher frequency of DN TCRgammadelta(+) cells in the presence of the AHR-activating ligand TCDD. We detected a novel population of CD25(int/lo)CD44(hi) cells associated with preferential emigration and a TCRgammadelta(+) T cell fate of thymocytes. Sorted DN TCRgammadelta(+) emigrants could proliferate if IL-2 was available. Moreover, they suppressed the proliferation of co-cultivated, activated CD4(+) T cells. Gene expression profiles of purified DN emigrants from TCDD*FTOC revealed 295 modulated genes, 10% of which are genes of the immune system. For instance, RAG-1, TdT, and Gfi-1 were downregulated, yet genes indicative of mature thymocytes were upregulated. In conclusion, we have detected changes in the differentiation programme of fetal DN thymocytes after ligand-activation of the AHR. In particular, we observed a higher frequency of DN TCRgammadelta(+) cells with high emigration potential, and possible regulatory functions.

  16. The Ikaros transcription factor regulates responsiveness to IL-12 and expression of IL-2 receptor alpha in mature, activated CD8 T cells.

    Directory of Open Access Journals (Sweden)

    Eric T Clambey

    Full Text Available The Ikaros family of transcription factors is critical for normal T cell development while limiting malignant transformation. Mature CD8 T cells express multiple Ikaros family members, yet little is known about their function in this context. To test the functions of this gene family, we used retroviral transduction to express a naturally occurring, dominant negative (DN isoform of Ikaros in activated CD8 T cells. Notably, expression of DN Ikaros profoundly enhanced the competitive advantage of activated CD8 T cells cultured in IL-12, such that by 6 days of culture, DN Ikaros-transduced cells were 100-fold more abundant than control cells. Expression of a DN isoform of Helios, a related Ikaros-family transcription factor, conferred a similar advantage to transduced cells in IL-12. While DN Ikaros-transduced cells had higher expression of the IL-2 receptor alpha chain, DN Ikaros-transduced cells achieved their competitive advantage through an IL-2 independent mechanism. Finally, the competitive advantage of DN Ikaros-transduced cells was manifested in vivo, following adoptive transfer of transduced cells. These data identify the Ikaros family of transcription factors as regulators of cytokine responsiveness in activated CD8 T cells, and suggest a role for this family in influencing effector and memory CD8 T cell differentiation.

  17. Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy.

    Science.gov (United States)

    Rossi, Luigi; Nicoletti, Maria Celeste; Carmosino, Monica; Mastrofrancesco, Lisa; Di Franco, Antonella; Indrio, Francesca; Lella, Rossella; Laviola, Luigi; Giorgino, Francesco; Svelto, Maria; Gesualdo, Loreto; Procino, Giuseppe

    2017-01-01

    Diabetic nephropathy (DN) is a microangiopathic complication of diabetes mellitus (DM) affecting one-third of diabetic patients. The large variability in the clinical presentation of renal involvement in patients with DM makes kidney biopsy a prerequisite for a correct diagnosis. However, renal biopsy is an invasive procedure associated with risk of major complications. Numerous studies aimed to identify a noninvasive biomarker of DN but, so far, none of these is considered to be sufficiently specific and sensitive. Water channel aquaporins (AQPs), expressed at the plasma membrane of epithelial tubular cells, are often dysregulated during DN. In this work, we analyzed the urine excretion of AQP5 and AQP2 (uAQP5 and uAQP2), via exosomes, in 35 diabetic patients: 12 normoalbuminuric with normal renal function (DM), 11 with proteinuric nondiabetic nephropathy (NDN), and 12 with histological diagnosis and classification of DN. ELISA and WB analysis independently showed that uAQP5 was significantly increased in DN patients. Interestingly, linear regression analysis showed a positive correlation between uAQP5 and the histological class of DN. The same analysis, focusing on uAQP2, showed comparable results. Taken together, these data suggest a possible use of AQP5 and AQP2 as novel noninvasive biomarkers to help in classifying the clinical stage of DN.

  18. Urinary Excretion of Kidney Aquaporins as Possible Diagnostic Biomarker of Diabetic Nephropathy

    Directory of Open Access Journals (Sweden)

    Luigi Rossi

    2017-01-01

    Full Text Available Diabetic nephropathy (DN is a microangiopathic complication of diabetes mellitus (DM affecting one-third of diabetic patients. The large variability in the clinical presentation of renal involvement in patients with DM makes kidney biopsy a prerequisite for a correct diagnosis. However, renal biopsy is an invasive procedure associated with risk of major complications. Numerous studies aimed to identify a noninvasive biomarker of DN but, so far, none of these is considered to be sufficiently specific and sensitive. Water channel aquaporins (AQPs, expressed at the plasma membrane of epithelial tubular cells, are often dysregulated during DN. In this work, we analyzed the urine excretion of AQP5 and AQP2 (uAQP5 and uAQP2, via exosomes, in 35 diabetic patients: 12 normoalbuminuric with normal renal function (DM, 11 with proteinuric nondiabetic nephropathy (NDN, and 12 with histological diagnosis and classification of DN. ELISA and WB analysis independently showed that uAQP5 was significantly increased in DN patients. Interestingly, linear regression analysis showed a positive correlation between uAQP5 and the histological class of DN. The same analysis, focusing on uAQP2, showed comparable results. Taken together, these data suggest a possible use of AQP5 and AQP2 as novel noninvasive biomarkers to help in classifying the clinical stage of DN.

  19. Neurophysiological and clinical effects of dry needling in patients with upper trapezius myofascial trigger points.

    Science.gov (United States)

    Abbaszadeh-Amirdehi, Maryam; Ansari, Noureddin Nakhostin; Naghdi, Soofia; Olyaei, Gholamreza; Nourbakhsh, Mohammad Reza

    2017-01-01

    Dry needling (DN) is a widely used in treatment of myofascial trigger points (MTrPs). The purpose of this pretest-posttest clinical trial was to investigate the neurophysiological and clinical effects of DN in patients with MTrPs. A sample of 20 patients (3 man, 17 women; mean age 31.7 ± 10.8) with upper trapezius MTrPs received one session of deep DN. The outcomes of neuromuscular junction response (NMJR), sympathetic skin response (SSR), pain intensity (PI) and pressure pain threshold (PPT) were measured at baseline and immediately after DN. There were significant improvements in SSR latency and amplitude, pain, and PPT after DN. The NMJR decreased and returned to normal after DN. A single session of DN to the active upper trapezius MTrP was effective in improving pain, PPT, NMJR, and SSR in patients with myofascial trigger points. Further studies are needed. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Urinary Markers of Tubular Injury in Early Diabetic Nephropathy

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    Temesgen Fiseha

    2016-01-01

    Full Text Available Diabetic nephropathy (DN is a common and serious complication of diabetes associated with adverse outcomes of renal failure, cardiovascular disease, and premature mortality. Early and accurate identification of DN is therefore of critical importance to improve patient outcomes. Albuminuria, a marker of glomerular involvement in early renal damage, cannot always detect early DN. Thus, more sensitive and specific markers in addition to albuminuria are needed to predict the early onset and progression of DN. Tubular injury, as shown by the detection of tubular injury markers in the urine, is a critical component of the early course of DN. These urinary tubular markers may increase in diabetic patients, even before diagnosis of microalbuminuria representing early markers of normoalbuminuric DN. In this review we summarized some new and important urinary markers of tubular injury, such as neutrophil gelatinase associated lipocalin (NGAL, kidney injury molecule-1 (KIM-1, liver-type fatty acid binding protein (L-FABP, N-acetyl-beta-glucosaminidase (NAG, alpha-1 microglobulin (A1M, beta 2-microglobulin (B2-M, and retinol binding protein (RBP associated with early DN.