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Sample records for pinus radiata usando

  1. 90SR uptake by Pinus ponderosa and Pinus radiata seedlings inoculated with ectomycorrhizal fungi

    International Nuclear Information System (INIS)

    Entry, J.A.; Emmingham, W.H.; Rygiewicz, P.T.

    1994-01-01

    Strontium-90 ( 90 Sr) is a radionuclide characteristic of fallout from nuclear reactor accidents and nuclear weapons testing. Prior studies have shown that Pinus ponderosa and P. radiata seedlings can remove appreciable quantities of 90 Sr from soil and store it in plant tissue. In this study, we inoculated P. ponderosa and P. radiata seedlings with one of five isolates of ectomycorrhizal fungi. Inoculated and noninoculated (control) seedlings were compared for their ability to remove 90 Sr from an organic growth medium. Ectomycorrhizal P. ponderosa and P. radiata seedlings are able to remove 3-5 times more 90 Sr from contaminated soil than seedlings without ectomycorrhizae. (Author)

  2. Biocontrol of Fusarium circinatum Infection of Young Pinus radiata Trees

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    Eugenia Iturritxa

    2017-01-01

    Full Text Available Pitch canker, caused by the fungus Fusarium circinatum, is a major disease of Pinus radiata currently controlled to some extent in nurseries by good hygiene and application of synthetic fungicides. The aim of this study was to evaluate alternative strategies to control fungal infections in nurseries and young pine plantations. The antagonistic effects of biocontrol bacteria and essential oils against F. circinatum in vitro and in young P. radiata trees were assessed. Pseudomonas fluorescens, Erwinia billingiae, and Bacillus simplex reduced the growth of the fungus in vitro by 17%–29%, and decreased the density of the mycelial mat. In young P. radiata trees, the length of F. circinatum lesions was reduced by 22%–25% by the same bacterial strains. Direct application of cinnamon and/or clove essential oils to wounds in stems of two-year-old P. radiata trees also limited the damage caused by F. circinatum. Lesion length was reduced by 51% following treatment with cinnamon oil (10% v/v, and by 45% following treatment with clove oil (15% v/v or a combination of both oils. However, the oils were toxic to younger trees. The biocontrol bacteria and essential oils show promise as prophylactic treatments to reduce the devastating effects of F. circinatum on P. radiata.

  3. Influencia de la poda en el desarrollo de masas de Pinus radiata D. Don y Pinus pinaster Aiton en Asturias

    OpenAIRE

    Hevia Cabal, Andrea

    2013-01-01

    Esta tesis evaluó la influencia de diferentes intensidades de poda sobre el crecimiento, desarrollo y persistencia de masas regulares jóvenes de Pinus radiata D. Don y Pinus pinaster Aiton en Asturias, dentro de una selvicultura sostenible enfocada a la producción de madera de calidad.

  4. A comparison of estimation methods for fitting Weibull, Johnson's SB and beta functions to Pinus pinaster, Pinus radiata and Pinus sylvestris stands in northwest Spain

    Energy Technology Data Exchange (ETDEWEB)

    Gorgoseo, J. J.; Rojo, A.; Camara-Obregon, A.; Dieguez-Aranda, U.

    2012-07-01

    The purpose of this study was to compare the accuracy of the Weibull, Johnson's SB and beta distributions, fitted with some of the most usual methods and with different fixed values for the location parameters, for describing diameter distributions in even-aged stands of Pinus pinaster, Pinus radiata and Pinus sylvestris in northwest Spain. A total of 155 permanent plots in Pinus sylvestris stands throughout Galicia, 183 plots in Pinus pinaster stands throughout Galicia and Asturias and 325 plots in Pinus radiata stands in both regions were measured to describe the diameter distributions. Parameters of the Weibull function were estimated by Moments and Maximum Likelihood approaches, those of Johnson's SB function by Conditional Maximum Likelihood and by Knoebel and Burkhart's method, and those of the beta function with the method based on the moments of the distribution. The beta and the Johnson's SB functions were slightly superior to Weibull function for Pinus pinaster stands; the Johnson's SB and beta functions were more accurate in the best fits for Pinus radiata stands, and the best results of the Weibull and the Johnson's SB functions were slightly superior to beta function for Pinus sylvestris stands. However, the three functions are suitable for this stands with an appropriate value of the location parameter and estimation of parameters method. (Author) 44 refs.

  5. Polyamine levels during the development of zygotic and somatic embryos of Pinus radiata

    Science.gov (United States)

    Rakesh Minocha; Dale R. Smith; Cathie Reeves; Kevin D. Steele; Subhash C. Minocha

    1999-01-01

    Changes in the cellular content of three polyamines (putrescine, spermidine and spermine) were compared at different stages of development in zygotic and somatic embryos of Pinus radiata D. Don. During embryo development, both the zygotic and the somatic embryos showed a steady increase in spermidine content, with either a small decrease or no...

  6. Climate as possible reproductive barrier in Pinus radiata (D. Don interspecific hybridisation

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    Hannél Ham

    2017-12-01

    Full Text Available Historically, interspecific hybridisation with Pinus radiata D. Don had limited success. The effect of environmental conditions and position of pollination bags in the tree were investigated as possible hybridisation barriers. The study was conducted in a P. radiata seed orchard in the Southern Cape (South Africa. Field data were compared to the climatic conditions at natural and commercial provenances of seven Mesoamerican Pinus species identified as possible hybrid partners. In vitro pollen studies were used to confirm whether interspecific crosses with P. radiata might be feasible within predefined climatic parameters. The temperature ranges for both top and northern side of P. radiata pine trees in the seed orchard was similar to the natural distribution of P. radiata, P. elliottii Engelm. and P. taeda L. in the USA. Results suggested that pollen of P. elliottii and P. taeda might be more suited to result in the successful pollination of P. radiata than the other Mesoamerican pine species tested in this study.  Furthermore, the combination of minimum temperature and precipitation also showed a closer correlation to successful hybridisation with P. radiata for both P. elliotii and P. taeda. However, pollen tube elongation studies did not support these results, suggesting that mean temperature might not be the only determining factor of hybridisation success. Three circadian temperature models that mimic natural conditions were developed for Karatara and Sabie (Tweefontein, Witklip and Spitskop.  These models will be tested in future in vitro studies to further evaluate temperature fluctuations between day and night regimes as a possible reproductive barrier limiting hybridisation success between P. radiata and other Mesoamerican pine species.

  7. Application of gamma radiation to the nodes detection in Pinus Radiata (D.Don) wood pieces

    International Nuclear Information System (INIS)

    Morales, J.R.; Dinator, Maria I; Karsulovic C, Jose T.; Leon G, Adolfo

    1996-01-01

    Attenuation of 59.5 KeV photons provided by an Am-241 source, has been used to detect knots in lumber pieces from Pinus Radiata (D.Don). It is shown that the linear attenuation coefficient is a sensitive parameter to detect singularities in the structure of this material. The scanning of the piece provides profiles which define the position and extension of the singularity. (author)

  8. Immunolocalization of IAA and ABA in roots and needles of radiata pine (Pinus radiata) during drought and rewatering.

    Science.gov (United States)

    De Diego, N; Rodríguez, J L; Dodd, I C; Pérez-Alfocea, F; Moncaleán, P; Lacuesta, M

    2013-05-01

    Anatomical, physiological and phytohormonal changes involved in drought tolerance were examined in different Pinus radiata D. Don breeds subjected to soil drying and rewatering. Breeds with the smallest stomatal chamber size had the lowest transpiration rate and the highest intrinsic water-use efficiency. Xylem cell size was positively correlated with leaf hydraulic conductance and needle indole-3-acetic acid (IAA) concentrations, whereas transpiration rate was negatively correlated with needle abscisic acid (ABA) levels. Since these two phytohormones seem important in regulating the P. radiata drought response, they were simultaneously immunolocalized in roots and needles of the most tolerant breed (P. radiata var. radiata × var. cedrosensis) during two sequential drought cycles and after rewatering. During drought, IAA was unequally distributed into the pointed area of the needle cross-section and mainly located in mesophyll and vascular tissue cells of needles, possibly inducing needle epinasty, whereas ABA was principally located in guard cells, presumably to elicit stomata closure. In the roots, at the end of the first drought cycle, while strong IAA accumulation was observed in the cortex, ABA levels decreased probably due to translocation to the leaves. Rewatering modified the distribution of both IAA and ABA in the needles, causing an accumulation principally in vascular tissue, with residual concentrations in mesophyll, likely favouring the acclimatization of the plants for further drought cycles. Contrarily, in the roots IAA and ABA were located in the exodermis, a natural barrier that regulates the phytohormone translocation to other plant tissues and hormone losses to the soil solution after rewatering. These results confirm that immunolocalization is an efficient tool to understand the translocation of IAA and ABA in plants subjected to different water stress situations, and clarify their role in regulating physiological responses such as stomata

  9. Relationship between fiber porosity and cellulose digestibility in steam-exploded Pinus radiata

    Energy Technology Data Exchange (ETDEWEB)

    Wong, K.K.Y.; Deverell, K.F.; Mackie, K.L.; Clark, T.A.; Donaldson, L.A.

    1988-04-05

    The use of lignocellulosic materials in bioconversion processes may be improved if the critical factors limiting conversion are better understood. Steam explosion after sulfur dioxide impregnation of wood chips is an effective method for improving the enzymatic digestibility of cellulose in the softwood Pinus radiata. Digestibility of pretreated fiber was progressively increased by altering the conditions of steam explosion. With increasing digestibility, there was an observed increase in fiber porosity as measured by the solute exclusion technique. Accessible pore volume and accessible surface area to a 5-nm dextran probe positively correlated with both 2- and 24-h digestion yields from pretreated fiber. The increase in accessibility was probably the result of hemicellulose extraction and lignin redistribution. A subsequent loss in accessibility, brought about by structural collapse or further lignin redistribution, resulted in a corresponding loss in digestibility. It appears that steam explosion increases cellulose digestibility in P. radiata by increasing fiber porosity.

  10. Above-ground biomass equations for Pinus radiata D. Don in Asturias

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    E. Canga

    2013-12-01

    Full Text Available Aim of the study: The aim of this study was to develop a model for above-ground biomass estimation for Pinus radiata D. Don in Asturias.Area of study: Asturias (NE of Spain.Material and methods: Different models were fitted for the different above-ground components and weighted regression was used to correct heteroscedasticity. Finally, all the models were refitted simultaneously by use of Nonlinear Seemingly Unrelated Regressions (NSUR to ensure the additivity of biomass equations.Research highlights: A system of four biomass equations (wood, bark, crown and total biomass was develop, such that the sum of the estimations of the three biomass components is equal to the estimate of total biomass. Total and stem biomass equations explained more than 92% of observed variability, while crown and bark biomass equations explained 77% and 89% respectively.Keywords: radiata pine; plantations; biomass.

  11. IDENTIFICACION DEL CILINDRO NUDOSO EN IMÁGENES TC DE TROZAS PODADAS DE PINUS RADIATA UTILIZANDO REDES NEURONALES ARTIFICIALES

    OpenAIRE

    Rojas Espinoza,Gerson; Ortiz Irribarren,Oscar

    2010-01-01

    La factibilidad de identificar el cilindro nudoso en imágenes de tomografía computarizada de rayos X (TC) de trozas podadas de pino radiata (Pinus radiata D. Don), fue evaluada utilizando un método de clasificación supervisada basado en Redes Neuronales Artificiales (RNA). El proceso de clasificación consideró también la identificación de la zona libre de defectos y nudos. Treinta trozas podadas de pino radiata fueron escaneadas en un escáner médico multi-slice de rayos X, donde las imágenes ...

  12. Metabolome Integrated Analysis of High-Temperature Response in Pinus radiata

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    Mónica Escandón

    2018-04-01

    Full Text Available The integrative omics approach is crucial to identify the molecular mechanisms underlying high-temperature response in non-model species. Based on future scenarios of heat increase, Pinus radiata plants were exposed to a temperature of 40°C for a period of 5 days, including recovered plants (30 days after last exposure to 40°C in the analysis. The analysis of the metabolome using complementary mass spectrometry techniques (GC-MS and LC-Orbitrap-MS allowed the reliable quantification of 2,287 metabolites. The analysis of identified metabolites and highlighter metabolic pathways across heat time exposure reveal the dynamism of the metabolome in relation to high-temperature response in P. radiata, identifying the existence of a turning point (on day 3 at which P. radiata plants changed from an initial stress response program (shorter-term response to an acclimation one (longer-term response. Furthermore, the integration of metabolome and physiological measurements, which cover from the photosynthetic state to hormonal profile, suggests a complex metabolic pathway interaction network related to heat-stress response. Cytokinins (CKs, fatty acid metabolism and flavonoid and terpenoid biosynthesis were revealed as the most important pathways involved in heat-stress response in P. radiata, with zeatin riboside (ZR and isopentenyl adenosine (iPA as the key hormones coordinating these multiple and complex interactions. On the other hand, the integrative approach allowed elucidation of crucial metabolic mechanisms involved in heat response in P. radiata, as well as the identification of thermotolerance metabolic biomarkers (L-phenylalanine, hexadecanoic acid, and dihydromyricetin, crucial metabolites which can reschedule the metabolic strategy to adapt to high temperature.

  13. Madera de compresión en Pinus radiata III: propiedades de pulpas kraft

    OpenAIRE

    Diaz-vaz,J.E; Ananias,R. A; Valenzuela,L; Torres,M; Rodriguez,S

    2012-01-01

    Estudios anteriores en estos mismos árboles de Pinus radiata con fustes levemente inclinados, demostraron que la madera de compresión leve que ellos exhibían presentaba: traqueidas más cortas, mayor densidad, mayor proporción de lignina, mayor proporción de extraíbles en etanol tolueno y menor proporción de alfa celulosa que la madera opuesta y que la madera de árboles sin madera de compresión. En el presente estudio se analiza las propiedades pulpables de la madera de compresión y la de sus ...

  14. Response in water yield to the thinning of Pinus radiata, Pinus patula and Eucalyptus grandis plantations

    CSIR Research Space (South Africa)

    Lesch, W

    1997-12-15

    Full Text Available . radiata plantation in the Biesievlei catchment, annual stream flow increased by between 10 and 71% (19-99 mm). These increases persisted for three and two years after the thinning, respectively. A final thinning in the same catchment removed only 22...

  15. Visual Grading and Structural Properties Assessment of Large Cross-Section Pinus radiata D. Don Timber

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    Eva Hermoso Prieto

    2016-04-01

    Full Text Available The use of large cross-section timber for structural purposes has increased in Spain, and knowledge of its properties is strategically necessary. The Spanish visual strength-grading standard UNE 56544 (2011 efficiency applied to large cross-section structural timber was analyzed using a sample of 363 specimens of radiata pine (Pinus radiata D. Don. from the Basque Country and Catalonia, Spain. Different sizes were tested (80 × 120 × 2400 mm3, 150 × 250 × 5600 mm3, 150 × 250 × 4300 mm3, and 200 × 250 × 5000 mm3. Bending strength, modulus of elasticity, and density were obtained, and characteristic values were determined in order to assign strength class according to European standard EN 338 (2010. Knots and twists were the most relevant singularities for visual strength grading. It was concluded that large cross-section Spanish radiata pine timber was suitable for structures, and it was assigned to the C20 strength class.

  16. Proanthocyanidin-rich Pinus radiata bark extract inhibits mast cell-mediated anaphylaxis-like reactions.

    Science.gov (United States)

    Choi, Yun Ho; Song, Chang Ho; Mun, Sung Phil

    2018-02-01

    Mast cells play a critical role in the effector phase of immediate hypersensitivity and allergic reactions. Pinus radiata bark extract exerts multiple biological effects and exhibits immunomodulatory and antioxidant properties. However, its role in mast cell-mediated anaphylactic reactions has not been thoroughly investigated. In this study, we examined the effects of proanthocyanidin-rich water extract (PAWE) isolated from P. radiata bark on compound 48/80-induced or antidinitrophenyl (DNP) immunoglobulin E (IgE)-mediated anaphylaxis-like reactions in vivo. In addition, we evaluated the mechanism underlying the inhibitory effect of PAWE on mast cell activation, with a specific focus on histamine release, using rat peritoneal mast cells. PAWE attenuated compound 48/80-induced or anti-DNP IgE-mediated passive cutaneous anaphylaxis-like reactions in mice, and it inhibited histamine release triggered by compound 48/80, ionophore A23187, or anti-DNP IgE in rat peritoneal mast cells in vitro. Moreover, PAWE suppressed compound 48/80-elicited calcium uptake in a concentration-dependent manner and promoted a transient increase in intracellular cyclic adenosine-3',5'-monophosphate levels. Together, these results suggest that proanthocyanidin-rich P. radiata bark extract effectively inhibits anaphylaxis-like reactions. Copyright © 2017 John Wiley & Sons, Ltd.

  17. Effect of Pinus radiata derived biochars on soil sorption and desorption of phenanthrene

    International Nuclear Information System (INIS)

    Zhang Honghua; Lin Kunde; Wang Hailong; Gan, Jay

    2010-01-01

    Biochars are anthropogenic carbonaceous sorbent and their influences on the sorption of environmental contaminants need to be characterized. Here we evaluated the effect of Pinus radiata derived biochars on soil sorption and desorption of phenanthrene. Two biochars separately produced at 350 o C and 700 o C and three soils were tested. Biochar amendment generally enhanced the soil sorption of phenanthrene. The biochar produced at 700 o C generally showed a greater ability at enhancing a soil's sorption ability than that prepared at 350 o C. The single-step desorption measurement showed an apparent hysteresis in biochar-amended soils. After 28 d equilibration, the sorptive capacity of biochar-amended soil (with an organic carbon content of 0.16%) significantly decreased. This study clearly suggested that biochar application enhanced soil sorption of hydrophobic organic compounds, but the magnitude of enhancement depended on the preparation of biochars, the indigenous soil organic carbon levels, and the contact time between soil and biochar. - Pinus radiata derived biochars influence soil sorption and desorption of phenanthrene.

  18. First report of shoot blight and dieback caused by Diplodia pinea on Pinus pinaster and P. radiata trees in Tunisia

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    B.T. Linaldeddu

    2009-01-01

    Full Text Available Damage caused by Diplodia pinea on Pinus pinaster and P. radiata forests are reported for the fi rst time in Tunisia. The affected plants show shoot blight, canker and branch dieback. On decaying and dead branches pycnidia of D. pinea are observed. The fungus was repeatedly isolated from the bark of symptomatic branches. The results of pathogenicity tests confirm the virulence of D. pinea and the susceptibility of both Pinus species to infection.

  19. Family-site interaction in Pinus radiata: implications for progeny testing strategy and regionalised breeding in New Zealand.

    Science.gov (United States)

    G.R. Johnson; R.D. Brudon

    1990-01-01

    A progeny test of 170 open-pollinated families from second-generation plus trees of Pinus radiata was established on four sites in New Zealand in 1981. Two test sites were on volcanic purnice soils in the Central North Island region and two were on phosphate-retentive clay soils in the Northland region.Assessments of volume growth, stem straightness, mal-...

  20. Horse grazing systems: understory biomass and plant biodiversity of a Pinus radiata stand

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    Antonio Rigueiro-Rodríguez

    2012-02-01

    Full Text Available Horse grazing systems may affect productivity and biodiversity of understory developed under Pinus radiata D. Don silvopastoral systems, while acting as a tool to reduce the risk of fire. This study compared continuous and rotational grazing systems effect upon biomass, fractions of stem, sprouts, leaves and woody parts of Ulex europaeus L. and alpha (Species Richness, Shannon-Wiener and beta (Jaccard and Magurran biodiversity for a period of four years in a P. radiata silvopastoral system. The experiment consisted of a randomized block design of two treatments (continuous and rotational grazing. Biomass, and species abundances were measured - biodiversity metrics were calculated based on these results for a two years of grazing and two years of post-grazing periods. Both continuous and rotational grazing systems were useful tools for reducing biomass and, therefore, fire risk. The rotational grazing system caused damage to the U. europaeus shrub, limiting its recovery once grazing was stopped. However, the more intensive grazing of U. europaeus plants under rotational had a positive effect on both alpha and beta biodiversity indexes due to the low capacity of food selection in the whole plot rather than continuous grazing systems. Biomass was not affected by the grazing system; however the rotational grazing system is more appropriate to reduce U. europaeus biomass and therefore forest fire risk at a long term and to enhance pasture biodiversity than the continuous grazing system.

  1. Phase-change related epigenetic and physiological changes in Pinus radiata D. Don.

    Science.gov (United States)

    Fraga, Mario F; Cañal, Maria Jesús; Rodríguez, Roberto

    2002-08-01

    DNA methylation and polyamine levels were analysed before and after Pinus radiata D. Don. phase change in order to identify possible molecular and physiological phase markers. Juvenile individuals (without reproductive ability) were characterised by a degree of DNA methylation of 30-35% and a ratio of free polyamines to perchloric acid-soluble polyamine conjugates greater than 1, while mature trees (with reproductive ability) had 60% 5-methylcytosine and a ratio of free polyamines to perchloric acid-soluble polyamine conjugates of less than 1. Results obtained with trees that attained reproductive capacity during the experimental period confirmed that changes in the degree of DNA methylation and polyamine concentrations found among juvenile and mature states come about immediately after the phase change. We suggest that both indicators may be associated with the loss of morphogenic ability during ageing, particularly after phase change, through a number of molecular interactions, which are subsequently discussed.

  2. Genomic DNA methylation-demethylation during aging and reinvigoration of Pinus radiata.

    Science.gov (United States)

    Fraga, Mario F; Rodríguez, Roberto; Cañal, Maria Jesús

    2002-08-01

    In animals, DNA methylation is related to gene silencing during ontogenic development. Little is known about DNA methylation in plants, although occasional changes in the DNA methylation state of specific gene promoters have been reported in angiosperms during some developmental processes. We found large differences in the extent of DNA methylation between meristematic areas of juvenile and mature Pinus radiata D. Don. trees, whereas differences in the extent of DNA methylation between differentiated tissues of juvenile and mature trees were small. In meristematic areas, there was a gradual decrease in extent of DNA methylation as the degree of reinvigoration increased. The observed changes in extent of DNA methylation during aging and reinvigoration indicate that reinvigoration could be a consequence of epigenetic modifications opposite in direction to those that occur during aging.

  3. Lactarius deliciosus and Pinus radiata in New Zealand: towards the development of innovative gourmet mushroom orchards.

    Science.gov (United States)

    Guerin-Laguette, Alexis; Cummings, Nicholas; Butler, Ruth Catherine; Willows, Anna; Hesom-Williams, Nina; Li, Shuhong; Wang, Yun

    2014-10-01

    The cultivation of Lactarius deliciosus (saffron milk cap) in New Zealand began in 2002 when fruiting bodies were produced in an Otago plantation of Pinus radiata seedlings artificially mycorrhized by L. deliciosus. In 2007, 42 P. radiata seedlings mycorrhized by L. deliciosus under controlled conditions were planted in a grass field at Plant and Food Research (Lincoln, Canterbury). The effects of pine bark mulch application and initial degree of mycorrhization of seedlings were examined to determine their influence on tree growth, development of mycorrhizae (i.e. their multiplication on the root system and their degree of branching) and fruiting body production. Mulch application increased tree growth significantly over 4 years. High initial mycorrhization slightly stimulated tree growth over 2 years. The initial degree of mycorrhization was positively, but not strongly, related to the persistence and development of L. deliciosus mycorrhizae and rhizomorphs based on root sample analyses 2 years after planting. However, mulching strongly reduced the proportion of highly branched L. deliciosus mycorrhizae compared with poorly ramified ones. A positive correlation was observed between the fruiting of L. deliciosus and the development of mycorrhizae. Mulching delayed the onset of fruiting body production. In 2010, fruiting bodies were produced only from non-mulched trees with eight of these (38 %) producing a total of 12 fruiting bodies. In 2011, 19 non-mulched trees (90 %) and 9 mulched trees (45 %) produced 143 and 47 fruiting bodies, respectively, totalling 190 fruiting bodies. By 2012, 19 non-mulched trees (90 %) and 13 mulched trees (65 %) produced 333 and 236 fruiting bodies, respectively, totalling 569 fruiting bodies (c. 30 kg). This study presents new information on factors influencing the onset of fruiting and the development of yields in a plantation of P. radiata mycorrhized by L. deliciosus. Projected yields as high as c. 300 kg/ha from the

  4. EFFECT OF THE REACTION CONDITIONS OVER THE YIELD AND PROPERTIES OF METHYLCELLULOSE OBTAINED FROM PINUS RADIATA KRAFT BLEACHED CELLULOSE

    OpenAIRE

    TAPIA, C.; SAPAG-HAGAR, J.; ANDRADE, C.T.; HASSÓN, J.; VALENZUELA, F.; BASUALTO, C.

    2002-01-01

    Kraft bleached cellulose from Pinus radiata was submitted to methylation reactions in heterogeneous media. After activation with 29% (w/w) NaOH solution, methylation reactions were carried out both with dimethyl sulfate (DMS) and methyl iodide (MI). The effects of the pressing ratio, reaction time and type of methylation agent were studied. The better yields were obtained with dimethylsulphate and the pressing ratio and reaction time were not signficant over the yield. Methylcellulose (MC) of...

  5. Effect of Partial Pre-Extraction of Hemicelluloses on the Properties of Pinus radiata Chemimechanical Pulps

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    Pablo Reyes

    2015-09-01

    Full Text Available Extraction of hemicelluloses prior to pulping and conversion of the extracted hemicelluloses to other bioproducts could provide additional revenue to traditional pulp and paper industries. The effect of hemicelluloses pre-extraction with a hydrothermal (HT process on Pinus radiata chemimechanical pulp (CMP properties was investigated in this study. The HT extraction resulted in a release of 7% to 58% of the initial amount of hemicelluloses from the wood. The extraction yield increased with temperature and extraction time. This hemicellulosic fraction was in the form of low molar mass oligomers with molecular weights varying from 1.5 to 100 kDa. Compared with the control (unextracted CMP pulp, the HT pre-extraction significantly reduced the refining energy to obtain a given fibrillation degree (freeness. The pulp yield with the HT/CMP process was in the range of 56% to 75%. Fiber properties of the pulps from pre-extracted wood, such as fiber length, were reduced, while increases in fiber width, fines content, fiber coarseness, and kink index were observed in comparison with the control pulps. The strength properties of CMP pulps decreased with increasing amounts of hemicellulose removal during the stage prior to pulping.

  6. Cellular modelling of secondary radial growth in conifer trees: application to Pinus radiata (D. Don).

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    Forest, Loïc; Demongeot, Jacques; Demongeota, Jacques

    2006-05-01

    The radial growth of conifer trees proceeds from the dynamics of a merismatic tissue called vascular cambium or cambium. Cambium is a thin layer of active proliferating cells. The purpose of this paper was to model the main characteristics of cambial activity and its consecutive radial growth. Cell growth is under the control of the auxin hormone indole-3-acetic. The model is composed of a discrete part, which accounts for cellular proliferation, and a continuous part involving the transport of auxin. Cambium is modeled in a two-dimensional cross-section by a cellular automaton that describes the set of all its constitutive cells. Proliferation is defined as growth and division of cambial cells under neighbouring constraints, which can eliminate some cells from the cambium. The cell-growth rate is determined from auxin concentration, calculated with the continuous model. We studied the integration of each elementary cambial cell activity into the global coherent movement of macroscopic morphogenesis. Cases of normal and abnormal growth of Pinus radiata (D. Don) are modelled. Abnormal growth includes deformed trees where gravity influences auxin transport, producing heterogeneous radial growth. Cross-sectional microscopic views are also provided to validate the model's hypothesis and results.

  7. Suppression of CCR impacts metabolite profile and cell wall composition in Pinus radiata tracheary elements.

    Science.gov (United States)

    Wagner, Armin; Tobimatsu, Yuki; Goeminne, Geert; Phillips, Lorelle; Flint, Heather; Steward, Diane; Torr, Kirk; Donaldson, Lloyd; Boerjan, Wout; Ralph, John

    2013-01-01

    Suppression of the lignin-related gene cinnamoyl-CoA reductase (CCR) in the Pinus radiata tracheary element (TE) system impacted both the metabolite profile and the cell wall matrix in CCR-RNAi lines. UPLC-MS/MS-based metabolite profiling identified elevated levels of p-coumaroyl hexose, caffeic acid hexoside and ferulic acid hexoside in CCR-RNAi lines, indicating a redirection of metabolite flow within phenylpropanoid metabolism. Dilignols derived from coniferyl alcohol such as G(8-5)G, G(8-O-4)G and isodihydrodehydrodiconiferyl alcohol (IDDDC) were substantially depleted, providing evidence for CCR's involvement in coniferyl alcohol biosynthesis. Severe CCR suppression almost halved lignin content in TEs based on a depletion of both H-type and G-type lignin, providing evidence for CCR's involvement in the biosynthesis of both lignin types. 2D-NMR studies revealed minor changes in the H:G-ratio and consequently a largely unchanged interunit linkage distribution in the lignin polymer. However, unusual cell wall components including ferulate and unsaturated fatty acids were identified in TEs by thioacidolysis, pyrolysis-GC/MS and/or 2D-NMR in CCR-RNAi lines, providing new insights into the consequences of CCR suppression in pine. Interestingly, CCR suppression substantially promoted pyrolytic breakdown of cell wall polysaccharides, a phenotype most likely caused by the incorporation of acidic compounds into the cell wall matrix in CCR-RNAi lines.

  8. Golgi enrichment and proteomic analysis of developing Pinus radiata xylem by free-flow electrophoresis.

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    Harriet T Parsons

    Full Text Available Our understanding of the contribution of Golgi proteins to cell wall and wood formation in any woody plant species is limited. Currently, little Golgi proteomics data exists for wood-forming tissues. In this study, we attempted to address this issue by generating and analyzing Golgi-enriched membrane preparations from developing xylem of compression wood from the conifer Pinus radiata. Developing xylem samples from 3-year-old pine trees were harvested for this purpose at a time of active growth and subjected to a combination of density centrifugation followed by free flow electrophoresis, a surface charge separation technique used in the enrichment of Golgi membranes. This combination of techniques was successful in achieving an approximately 200-fold increase in the activity of the Golgi marker galactan synthase and represents a significant improvement for proteomic analyses of the Golgi from conifers. A total of thirty known Golgi proteins were identified by mass spectrometry including glycosyltransferases from gene families involved in glucomannan and glucuronoxylan biosynthesis. The free flow electrophoresis fractions of enriched Golgi were highly abundant in structural proteins (actin and tubulin indicating a role for the cytoskeleton during compression wood formation. The mass spectrometry proteomics data associated with this study have been deposited to the ProteomeXchange with identifier PXD000557.

  9. Bark polyflavonoids from Pinus radiata as functional building-blocks for polylactic acid (PLA-based green composites

    Directory of Open Access Journals (Sweden)

    D. E. Garcia

    2016-10-01

    Full Text Available Polylactic acid (PLA was melt-blended with Pinus radiata unmodified and modified (hydroxypropyled bark polyflavonoids in order to use such polyphenolic building-blocks as functional additives for envisaged applications. Rheological, morphological, molecular, thermal, and flexural properties were studied. Polyflavonoids improved blend processability in terms of short-time mixing. Furthermore, hydroxypropylated polyflavonoids improve miscibility in binary and ternary blends. Blend-composition affects crystallization-, melting-, and glass transition-temperature of PLA, as well as thermal resistance, and flexural properties of the blends. Polyflavonoids induced PLA-crystallization, and polymer-chain decomposition. Modified and unmodified bark polyflavonoids from radiata pine can be used successfully in PLA-based green composites beyond the food-packaging applications. The high compatibility between PLA and hydroxypropyled polyflavonoids highlights the potential of such phenolic derivatives for PLA-based material design.

  10. Generation and analysis of expressed sequence tags from six developing xylem libraries in Pinus radiata D. Don

    Directory of Open Access Journals (Sweden)

    Dillon Shannon K

    2009-01-01

    Full Text Available Abstract Background Wood is a major renewable natural resource for the timber, fibre and bioenergy industry. Pinus radiata D. Don is the most important commercial plantation tree species in Australia and several other countries; however, genomic resources for this species are very limited in public databases. Our primary objective was to sequence a large number of expressed sequence tags (ESTs from genes involved in wood formation in radiata pine. Results Six developing xylem cDNA libraries were constructed from earlywood and latewood tissues sampled at juvenile (7 yrs, transition (11 yrs and mature (30 yrs ages, respectively. These xylem tissues represent six typical development stages in a rotation period of radiata pine. A total of 6,389 high quality ESTs were collected from 5,952 cDNA clones. Assembly of 5,952 ESTs from 5' end sequences generated 3,304 unigenes including 952 contigs and 2,352 singletons. About 97.0% of the 5,952 ESTs and 96.1% of the unigenes have matches in the UniProt and TIGR databases. Of the 3,174 unigenes with matches, 42.9% were not assigned GO (Gene Ontology terms and their functions are unknown or unclassified. More than half (52.1% of the 5,952 ESTs have matches in the Pfam database and represent 772 known protein families. About 18.0% of the 5,952 ESTs matched cell wall related genes in the MAIZEWALL database, representing all 18 categories, 91 of all 174 families and possibly 557 genes. Fifteen cell wall-related genes are ranked in the 30 most abundant genes, including CesA, tubulin, AGP, SAMS, actin, laccase, CCoAMT, MetE, phytocyanin, pectate lyase, cellulase, SuSy, expansin, chitinase and UDP-glucose dehydrogenase. Based on the PlantTFDB database 41 of the 64 transcription factor families in the poplar genome were identified as being involved in radiata pine wood formation. Comparative analysis of GO term abundance revealed a distinct transcriptome in juvenile earlywood formation compared to other stages of

  11. Evaluation of Trichoderma spp. and Clonostachys spp. Strains to Control Fusarium circinatum in Pinus radiata Seedlings Evaluación de Cepas de Trichoderma spp. y Clonostachys spp. para Controlar Fusarium circinatum en Plántulas de Pinus radiata

    Directory of Open Access Journals (Sweden)

    Priscila Moraga-Suazo

    2011-09-01

    Full Text Available The fungus Fusarium circinatum Nirenberg & O’Donnell causes pine pitch canker, an important disease for conifers worldwide. F. circinatum was first detected in Chile in 2001 and to date is present in nurseries and clonal hedges from Libertador General Bernardo O’Higgins Region to Los Rios Region. The purpose of this study was to evaluate the potential of Trichoderma spp. and Clonostachys spp. strains to control F. circinatum in Pinus radiata D. Don seedlings in the absence of other effective control methods. Eighty-one Trichoderma spp. and Clonostachys spp. strains were evaluated through in vitro assays to determine their ability to act as antagonists of F. circinatum and 21 strains were tested for their ability to reduce post-emergence mortality and increase P. radiata survival under greenhouse conditions. During in vitro experiments, 15 strains of Trichoderma inhibited mycelial growth of the pathogen by more than 60% and one strain of Clonostachys showed parasitism of F. circinatum hyphae. Greenhouse experiments showed no control of the disease when the antagonists were added to substrate after the pathogen. However, when the antagonists were added before the pathogen, four strains (Clonostachys UDC-32 and UDC-222 and Trichoderma UDC-23 and UDC-408 reduced post-emergence mortality between 80 and 100%. Among these strains, only Clonostachys UDC-222 significantly increased the survival of P. radiata seedlings. These results showed that Clonostachys UDC-222 has the potential to be used as a biocontrol agent against F. circinatum in the production of P. radiata plants.Fusarium circinatum Nirenberg & O’Donnell es el hongo que causa el cancro resinoso del pino, una enfermedad de importancia mundial en coníferas. En Chile, F. cicirnatum fue detectado por primera vez el año 2001 y a la fecha se encuentra presente en algunos viveros y huertos clonales desde la Región del Libertador General Bernardo O’Higgins hasta la Región de Los R

  12. Progress towards initiation of somatic embryogenesis from differentiated tissues of radiata pine (Pinus radiata D. Don) using cotyledonary embryos

    DEFF Research Database (Denmark)

    Find, Jens Iver; Hargreaves, Cathy L.; Reeves, Catherine B.

    2014-01-01

    of dissected embryos and a modified Litvay medium, Glitz, was best. This combination gave the highest rate of initiation, and it was possible to initiate somatic embryogenesis (SE) from differentiated cells in the epicotyledonary region of postcotyledonary zygotic embryos from the two tested families...... with an average initiation rate of approximately 24% and 7% from stage five and six embryos, respectively. This is different from established initiation protocols of embryogenic cultures in radiata pine, which has traditionally been based on embryo rescue and continued proliferation of immature zygotic embryos....... A further implication of initiation of SE from excised post-cotyledonary embryos was that the period of initiation of embryogenic cultures was extended from 4 to 12 wk....

  13. VARIABILIDAD GENÉTICA DEL CRECIMIENTO EN PROGENIES SELECTAS DE Pinus radiata

    Directory of Open Access Journals (Sweden)

    Freddy Mora

    2006-09-01

    Full Text Available Heredabilidad en sentido estricto (h2, correlación genética intra-clase (2IC y componentes de varianza para la altura, diámetro a la altura del pecho (DAP y volumen del fuste, a los siete años de edad después de la plantación, fueron analizados en dos ensayos de progenie de Pinus radiata establecidos en el sur de Chile, en orden a examinar la variabilidad genética del crecimiento en una generación avanzada de la especie. Estos ensayos incluyeron árboles provenientes de propagación vegetativa y generativa. Las heredabilidades fueron estimadas para los árboles originados de semillas, y mostraron los siguientes valores: altura, 0.08-0.13; diámetro a la altura del pecho, 0-0.13; volumen del fuste, 0-0.09. Los valores de la correlación genética intra-clase, estimados para el grupo de árboles originados de propagación vegetativa, fueron moderados a bajos (2IC: altura, 0.1-0.11; diámetro a la altura del pecho, 0.01-0.05; volumen del fuste, 0.03-0.06. No obstante la presencia de heredabilidades y correlaciones moderadas para algunas características y, la variación relativamente limitada entre grupos de árboles reflejan la fuerte selección realizada a las progenies. En general, los árboles provenientes de estacas presentaron una tendencia a la menor variación intra-genotípica, y evidenciaron magnitudes relativas de las varianzas debida al grupo (2G mayores a las varianzas familiares (2F, en cuatro de seis caracteres evaluados, indicando una tendencia a la mayor homogeneidad en el crecimiento, característica común de la propagación vegetativa.

  14. Seasonal changes in stable carbon isotope ratios within annual growth rings of Pinus radiata

    International Nuclear Information System (INIS)

    Walcroft, A.; Silvester, W.; Whitehead, D.; Kelliher, F.

    1997-01-01

    The stable isotope composition of photosynthetically assimilated carbon (δ 13 C) is determined by the ratio of the leaf internal CO 2 concentration (c i ) to that of the ambient air (c a ), and so reflects the contribution of both stomatal conductance (g s ) and the rate of photosynthesis (A). Assimilated carbon which is subsequently laid down as wood in annual growth rings may therefore represent a time integrated record of physiological responses by the whole tree to seasonal changes in the environmental variables regulating growth. We analysed the stable carbon isotope composition of Pinus radiata wood collected from two plantation forest sites in New Zealand which differ markedly in temperature, rainfall and soil characteristics. For both sites, discs were cut from the stem of several trees near ground level and whole wood samples were taken from within individual annual growth rings over a number of years. At one site, diameter bands were installed over the 1994 - 1996 growing seasons in order to date precisely the formation of wood during that time. Trees at each site consistently showed a seasonal pattern in the stable isotope composition of wood within individual growth rings. The amplitude of seasonal δ 13 C variation at the wet and dry sites were 1-2 per thousand and 4 per thousand respectively. Mean δ 13 C values from the wet site were 3 per thousand more 13 C depleted than those from the dry site implying lower water-use efficiency (carbon assimilation per unit transpiration). A process-based, model of stomatal conductance and CO 2 assimilation was combined with a soil-water balance model to estimate the average daily leaf-level intercellular CO 2 concentration (c i ). Over two growing seasons at each site there was generally good agreement between mean canopy-level c i derived from the tree-ring δ 13 C data and modelled leaf-level c i levels. Further, the ratio of annual CO 2 assimilation to transpiration estimated by the model for each site

  15. Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation

    DEFF Research Database (Denmark)

    Hargreaves, Cathy; Find, Jens; Reeves, Cathie

    2009-01-01

    The principal aim of this investigation was to improve somatic embryogenesis initiation and to enhance representation of families and genotypes within those families of Pinus radiata D. Don. A total of 19 open-pollinated seed families, many with unrelated and weakly related parents, were tested...

  16. Descomposición de hojarasca de Pinus radiata y tres especies arbóreas nativas Decomposition of leaf litter of Pinus radiata and three native tree species

    Directory of Open Access Journals (Sweden)

    CHRISTOPHER H. LUSK

    2001-09-01

    Full Text Available En el centro-sur de Chile, las últimas décadas han sido testigo de una conversión masiva de bosques, matorrales y tierras agrícolas a plantaciones de árboles exóticos. Aunque se ha estudiado la influencia de dichos cambios sobre el balance hídrico, los posibles efectos sobre otros procesos ecosistémicos han recibido poca atención. En esta breve comunicación se presentan datos de un estudio de la descomposición de hojarasca de Pinus radiata y tres especies arbóreas nativas, llevado a cabo con el fin de explorar los posibles efectos del reemplazo de bosque nativo por plantaciones exóticas sobre el ciclaje de nutrientes. Se incubaron muestras de las cuatro especies en dos ambientes distintos en sitios colindantes: bajo un bosque nativo secundario, y bajo un rodal de P. radiata. Se registró la pérdida de peso seco después de dos meses y seis meses. Las tasas diarias de descomposición fueron mucho mayores durante los primeros dos meses de incubación que durante los cuatro meses subsiguientes. En ambas fechas hubo diferencias significativas entre las especies y entre los sitios: todas las especies presentaron mayores tasas de descomposición bajo P. radiata que bajo el bosque nativo. No hubo evidencia de interacción entre sitio y especie. Después de seis meses, el orden de pérdida de peso seco fue Nothofagus obliqua > P. radiata > Peumus boldus > Cryptocarya alba. La variación interespecífica en la tasa de descomposición presentó más relación con el área foliar específica que con el contenido de nitrógeno en la hojarasca. Dado que la hojarasca de P. radiata se descompuso más lentamente que la de la especie caducifolia N. obliqua, pero más rápidamente que las especies esclerófilas, los efectos de la sustitución o invasión sobre descomposición dependerían de la composición original del bosque nativo en cuestiónRecent decades have seen widespread conversion of native forests, shrublands and farmland in south

  17. Application of gamma radiation to the nodes detection in Pinus Radiata (D.Don) wood pieces; Aplicacion de la radiacion gamma en la deteccion de nudos en piezas de madera de Pino Radiata (D.Don)

    Energy Technology Data Exchange (ETDEWEB)

    Morales, J R; Dinator, Maria I [Chile Univ., Santiago (Chile). Dept. de Fisica; Karsulovic C, Jose T; Leon G, Adolfo [Chile Univ., Santiago (Chile). Dept. de Tecnologia de la Madera

    1997-12-31

    Attenuation of 59.5 KeV photons provided by an Am-241 source, has been used to detect knots in lumber pieces from Pinus Radiata (D.Don). It is shown that the linear attenuation coefficient is a sensitive parameter to detect singularities in the structure of this material. The scanning of the piece provides profiles which define the position and extension of the singularity. (author). 7 refs.

  18. The effect of water availability on plastic responses and biomass allocation in early growth traits of Pinus radiata D. Don

    Energy Technology Data Exchange (ETDEWEB)

    Espinoza, S. E.; Magni, C. R.; Martinez, V. A.; Ivkovic, M.

    2013-05-01

    Aim of study: The aim of the study was to assess the effect of water availability on plastic responses and biomass allocation in early growth traits of Pinus radiata D. Don. Area of study: Seedlings of 69 families of P. radiata belonging to five different sites in Central Chile, ranging from coastal range to fothills of the Andes, were grown in controlled conditions to evaluate differences in response to watering. Material and methods: The seedlings were subjected to two watering regimes: well-watered treatment, in which seedlings were watered daily, and water stress treatment in which seedlings were subjected to three cyclic water deficits by watering to container capacity on 12 days cycles each. After twenty-eight weeks root collar diameter, height, shoot dry weight (stem + needles), root dry weight, total dry weight, height/diameter ratio and root/shoot ratio were recorded. Patterns and amounts of phenotypic changes, including changes in biomass allocation, were analyzed. Main results: Families from coastal sites presented high divergence for phenotypic changes, allocating more biomass to shoots, and those families from interior sites presented low phenotypic plasticity, allocating more biomass to roots at the expense of shoots. These changes are interpreted as a plastic response and leads to the conclusion that the local land race of P. radiata in Chile originating from contrasting environments possess distinct morphological responses to water deficit which in turn leads to phenotypic plasticity. Research highlights: Families belonging to sandy soil sites must be considered for tree breeding in dry areas, selecting those with high root: shoot ratio. (Author) 46 refs.

  19. MADERA DE COMPRESIÓN EN PINUS RADIATA D. DON: I, CARACTERÍSTICAS ANATÓMICAS

    OpenAIRE

    Diaz-vaz,J.E; Fernandez,A; Valenzuela,L; Torres,M

    2007-01-01

    Se caracterizó la madera de compresión en 10 árboles de Pinus radiata D.Don con inclinaciones menores a 10 º en dos sitios.          Los diámetros de traqueidas de los árboles del sitio Arena húmeda resultaron estadísticamente mayores que los del sitio Arcilla; pero no resultaron diferentes al comparar madera juvenil y madura o al compara madera de compresión con madera opuesta.          Diferencias significativas se determinaron en el largo de traqueida entre sitios (Arena húmeda-Arcilla), t...

  20. First record of the genus .i.Bloszykiella./i. in Kenya with the description of .i.Bloszykiella tertia./i. sp n. (Acari: Uropodidae) from a .i.Pinus radiata./i. D. Don plantation

    Czech Academy of Sciences Publication Activity Database

    Kontschán, J.; Starý, Josef

    2015-01-01

    Roč. 56, č. 3 (2015), s. 629-635 ISSN 1681-5556 Institutional support: RVO:60077344 Keywords : Acari * new species * Pinus radiata plantation * Kenya Subject RIV: EG - Zoology Impact factor: 0.708, year: 2015

  1. Formation of post-fire water-repellent layers in Monterrey pine (Pinus radiata D. Don) plantations in south-central Chile

    Science.gov (United States)

    P. Garcia-Chevesich; R. Pizarro; C. L. Stropki; P. Ramirez de Arellano; P. F. Ffolliott; L. F. DeBano; Dan Neary; D. C. Slack

    2010-01-01

    A wildfire burned about 15,000 ha of Monterrey Pine (Pinus radiata D. Don) plantations near Yungay, Chile, in January of 2007. Post-fire water repellency (hydrophobicity) was measured using the water-drop-penetration-time (WDPT) method at depths of 0, 5, and 10 mm from the soil surface. These measurements were collected on burned sites of both young (4-years old) and...

  2. Wood formation from the base to the crown in Pinus radiata: gradients of tracheid wall thickness, wood density, radial growth rate and gene expression

    Science.gov (United States)

    Sheree Cato; Lisa McMillan; Lloyd Donaldson; Thomas Richardson; Craig Echt; Richard Gardner

    2006-01-01

    Wood formation was investigated at five heights along the bole for two unrelated trees of Pinus radiataBoth trees showed clear gradients in wood properties from the base to the crown. Cambial cells at the base of the tree were dividing 3.3-fold slower than those at the crown, while the average thickness of cell walls in wood was highest at the base....

  3. Stable carbon and oxygen isotope ratios of annual rings of pinus radiata provide an integrative record of canopy gas exchange

    International Nuclear Information System (INIS)

    Barbour, M.M.; Farquhar, G.D.

    2000-01-01

    Full text: Seasonal variation in δ 13 C and δ 18 O of cellulose from annual rings of Pinus radiata growing at each of three sites in New Zealand was measured. The three sites differed in annual water balance, temperature, and vapour pressure deficit, and these differences were reflected in cellulose δ 13 C and δ 18 O. Specific events such as drought or heavy rain were recorded as peaks and troughs in enrichment. A canopy-level combined photosynthesis and conductance model was linked to a model of soil water content and δ 18 O of xylem water to allow daily prediction of δ 13 C and δ 18 O of cellulose. A photosynthesis-weighted average of δ 13 C and δ 18 O was calculated for each sampling period. Each sample represented between 3 and 30 days, depending on stem growth rate. The timing and amplitude of changes in δ 13 C were predicted accurately by the model, while general seasonal patterns and event related peaks in δ 18 O enrichment were well predicted. These results suggest that stable isotope ratios of cellulose from annual rings reflect the canopy response to interactions between site-specific and seasonal variation in climatic conditions and soil water availability

  4. The carbon budget of Pinus radiata plantations in south-western Australia under 4 climate change scenarios

    International Nuclear Information System (INIS)

    Simioni, G.; Ritson, P.; McGrath, J.; Dumbrell, I.; Copeland, B.

    2009-01-01

    The future stem wood production and net ecosystem production of Pinus radiata plantations in southwestern Australia were estimated in this modelling study, which was conducted in order to determine the potential effects of anticipated severe rainfall reductions in the region. Four climate change and emission scenarios were considered as well as simulations of the present climate. Results of the study showed that stem wood production and NEP were not significantly influenced by moderate changes in temperature. However, stem wood production and NEP decreased significantly under the most pessimistic climate change scenarios. Results of the study suggested that a trade-off between the positive effects of rising atmospheric carbon dioxide (CO 2 ) on plant and water use efficiency and the negative impacts of decreased rainfall and increased temperatures. Changes in heterotrophic respiration lagged behind changes in plant growth. It was concluded that realistic predictions of forest production and carbon sequestration potential will require modelling tools capable of characterizing interactions between environmental variables, plant physiology and soil organic matter decomposition, as well as the potential range of climate change scenarios. 53 refs., 4 tabs., 9 figs

  5. Fourier transform infrared imaging and microscopy studies of Pinus radiata pulps regarding the simultaneous saccharification and fermentation process

    Energy Technology Data Exchange (ETDEWEB)

    Castillo, Rosario del P., E-mail: rosariocastillo@udec.cl [Faculty of Pharmacy, University of Concepcion, Concepcion (Chile); Biotechnology Center, University of Concepcion, Concepcion (Chile); Araya, Juan [Faculty of Pharmacy, University of Concepcion, Concepcion (Chile); Biotechnology Center, University of Concepcion, Concepcion (Chile); Troncoso, Eduardo [Consorcio Bioenercel S.A, University of Concepcion, Concepcion (Chile); Vinet, Silenne; Freer, Juanita [Biotechnology Center, University of Concepcion, Concepcion (Chile); Faculty of Chemical Sciences, University of Concepcion, Concepcion (Chile)

    2015-03-25

    The distribution and chemical patterns of lignocellulosic components at microscopic scale and their effect on the simultaneous saccharification and fermentation process (SSF) in the production of bioethanol from Pinus radiata pulps were analyzed by the application of diverse microscopical techniques, including scanning electronic microscopy (SEM), confocal laser scanning microscopy (CLSM) and attenuated total reflectance (ATR) – Fourier transform infrared microspectroscopy. This last technique was accompanied with multivariate methods, including principal component analysis (PCA) and multivariate curve resolution with alternating least squares (MCR-ALS) to evaluate the distribution patterns and to generate pure spectra of the lignocellulosic components of fibers. The results indicate that the information obtained by the techniques is complementary (ultrastructure, confocality and chemical characterization) and that the distribution of components affects the SSF yield, identifying lignin coalescence droplets as a characteristic factor to increase the SSF yield. Therefore, multivariate analysis of the infrared spectra enabled the in situ identification of the cellulose, lignin and lignin-carbohydrates arrangements. These techniques could be used to investigate the lignocellulosic components distribution and consequently their recalcitrance in many applications where minimal sample manipulation and microscale chemical information is required.

  6. Toxicological features of maleilated polyflavonoids from Pinus radiata (D. Don.) as potential functional additives for biomaterials design.

    Science.gov (United States)

    García, Danny E; Medina, Paulina A; Zúñiga, Valentina I

    2017-11-01

    Polyflavonoids from Pinus radiata (D. Don.) are an abundant natural oligomers highly desirable as renewable chemicals. However, structural modification of polyflavonoids is a viable strategy in order to use such polyphenols as macrobuilding-blocks for biomaterial design. Polyflavonoids were esterified with three five-member cyclic anhydrides (maleic, itaconic, and citraconic) at 20 °C during 24 h in order to diversify physicochemical-, and biological-properties for agricultural, and food-packaging applications. In addition, the influence of the chemical modification, as well as the chemical structure of the grafting on toxicological features was evaluated. Structural features of derivatives were analyzed by spectroscopy (FT-IR and 1 H-NMR), and the degree of substitution was calculated. Toxicological profile was assessed by using three target species in a wide range of concentration (0.01-100 mgL - 1 ). Effect of polyflavonoids on the growth rate (Selenastrum capricornutum), mortality (Daphnia magna), and germination and radicle length (Lactuca sativa) was determined. Chemical modification affects the toxicological profile on the derivatives in a high extent. Results described remarkable differences in function of the target specie. The bioassays indicate differences of the polyflavonoids toxicological profile associated to the chemical structure of the grafting. Results allowed conclude that polyflavonoids from pine bark show slight toxic properties. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Distribution and movement of nutrients and metals in a Pinus radiata forest soil following applications of biosolids

    International Nuclear Information System (INIS)

    McLaren, Ronald G.; Clucas, Lynne M.; Speir, Tom W.; Schaik, Andrew P. van

    2007-01-01

    Samples of biosolids, spiked with increasing amounts of Cu, Ni or Zn were applied to field plots in a Pinus radiata forest, and the nutrient and metal status of the forest litter and underlying mineral soil was monitored over a period of six years following application. The macronutrient status of the forest litter was changed markedly by the biosolids application, with substantial increases in N, P and Ca concentrations, and decreases in Mg and K. The C/N ratio of the litter was also decreased and pH was increased by the biosolids application. The metals applied with the biosolids were retained predominantly in the litter layer, and even with non-metal-spiked biosolids there were substantial increases in litter metal concentrations. There was also firm evidence of some movement of Cu, Ni and Zn into the underlying mineral soil. The potential environmental issues resulting from these changes in nutrient and metal status are discussed. - Biosolids application to forest soils results in substantial build-up of macronutrients and metals in the forest litter layer

  8. Distribution and movement of nutrients and metals in a Pinus radiata forest soil following applications of biosolids

    Energy Technology Data Exchange (ETDEWEB)

    McLaren, Ronald G. [Centre for Soil and Environmental Quality, Agriculture and Life Sciences Division, P.O. Box 84, Lincoln University Canterbury (New Zealand)]. E-mail: mclaren@lincoln.ac.nz; Clucas, Lynne M. [Centre for Soil and Environmental Quality, Agriculture and Life Sciences Division, P.O. Box 84, Lincoln University Canterbury (New Zealand); Speir, Tom W. [Institute of Environmental Science and Research Ltd, P.O. Box 50348, Porirua (New Zealand); Schaik, Andrew P. van [Institute of Environmental Science and Research Ltd, P.O. Box 50348, Porirua (New Zealand)

    2007-05-15

    Samples of biosolids, spiked with increasing amounts of Cu, Ni or Zn were applied to field plots in a Pinus radiata forest, and the nutrient and metal status of the forest litter and underlying mineral soil was monitored over a period of six years following application. The macronutrient status of the forest litter was changed markedly by the biosolids application, with substantial increases in N, P and Ca concentrations, and decreases in Mg and K. The C/N ratio of the litter was also decreased and pH was increased by the biosolids application. The metals applied with the biosolids were retained predominantly in the litter layer, and even with non-metal-spiked biosolids there were substantial increases in litter metal concentrations. There was also firm evidence of some movement of Cu, Ni and Zn into the underlying mineral soil. The potential environmental issues resulting from these changes in nutrient and metal status are discussed. - Biosolids application to forest soils results in substantial build-up of macronutrients and metals in the forest litter layer.

  9. Fourier transform infrared imaging and microscopy studies of Pinus radiata pulps regarding the simultaneous saccharification and fermentation process

    International Nuclear Information System (INIS)

    Castillo, Rosario del P.; Araya, Juan; Troncoso, Eduardo; Vinet, Silenne; Freer, Juanita

    2015-01-01

    The distribution and chemical patterns of lignocellulosic components at microscopic scale and their effect on the simultaneous saccharification and fermentation process (SSF) in the production of bioethanol from Pinus radiata pulps were analyzed by the application of diverse microscopical techniques, including scanning electronic microscopy (SEM), confocal laser scanning microscopy (CLSM) and attenuated total reflectance (ATR) – Fourier transform infrared microspectroscopy. This last technique was accompanied with multivariate methods, including principal component analysis (PCA) and multivariate curve resolution with alternating least squares (MCR-ALS) to evaluate the distribution patterns and to generate pure spectra of the lignocellulosic components of fibers. The results indicate that the information obtained by the techniques is complementary (ultrastructure, confocality and chemical characterization) and that the distribution of components affects the SSF yield, identifying lignin coalescence droplets as a characteristic factor to increase the SSF yield. Therefore, multivariate analysis of the infrared spectra enabled the in situ identification of the cellulose, lignin and lignin-carbohydrates arrangements. These techniques could be used to investigate the lignocellulosic components distribution and consequently their recalcitrance in many applications where minimal sample manipulation and microscale chemical information is required

  10. Lack of genetic variation in tree ring delta13C suggests a uniform, stomatally-driven response to drought stress across Pinus radiata genotypes.

    Science.gov (United States)

    Rowell, Douglas M; Ades, Peter K; Tausz, Michael; Arndt, Stefan K; Adams, Mark A

    2009-02-01

    We assessed the variation in delta(13)C signatures of Pinus radiata D. Don stemwood taken from three genetic trials in southern Australia. We sought to determine the potential of using delta(13)C signatures as selection criteria for drought tolerance. Increment cores were taken from P. radiata and were used to determine the basal area increment and the delta(13)C signature of extracted cellulose. Both growth increment and cellulose delta(13)C were affected by water availability. Growth increment and delta(13)C were negatively correlated suggesting that growth was water-limited. While there was significant genetic variation in growth, there was no significant genetic variation in cellulose delta(13)C of tree rings. This suggests that different genotypes of P. radiata display significant differences in growth and yet respond similarly to drought stress. The delta(13)C response to drought stress was more due to changes in stomatal conductance than to the variation in photosynthetic capacity, and this may explain the lack of genetic variation in delta(13)C. The lack of genetic variation in cellulose delta(13)C of tree rings precludes its use as a selection criterion for drought tolerance among P. radiata genotypes.

  11. Silvicultural manipulation and site effect on above and belowground biomass equations for young Pinus radiata

    International Nuclear Information System (INIS)

    Rubilar, Rafael A.; Allen, H. Lee; Alvarez, Jose S.; Albaugh, Timothy J.; Fox, Thomas R.; Stape, Jose L.

    2010-01-01

    There is little understanding of how silvicultural treatments, during the early stages of tree development, affect allometric relationships. We developed and compared stem, branch, foliage, coarse and fine root biomass, and leaf area estimation equations, for four-year-old genetically improved radiata pine trees grown on three contrasting soil-site conditions. At each site, selected trees were destructively sampled from a control (shovel planted, no weed control, fertilized with 2 g of boron), a shovel planted + weed control (2 first years) + complete fertilization (nitrogen + phosphorus + boron 2 first years + potassium 2nd year), and a soil tillage (subsoil at 60 cm) + weed control (first 2 years) + complete fertilization treatment. Tissues were separated into foliage, branch, stem, fine and coarse roots (>2 mm). Regression equations for each tree biomass tissue versus leaf area were fit for each site and compared among treatments and sites with the same genetic material. Our results indicated that individual tree biomasses for young plantations are affected by silvicultural treatment and site growing conditions. Higher variability in estimates was found for foliage and branches due to the ephemeral nature of these components. Stem biomass equations vary less, but differences in biomass equations were found among sites and treatments. Coarse root biomass estimates were variable but less than expected, considering the gradient among sites. Similar to stem biomass, a simple positive general linear relationship between root collar diameter, or diameter at breast height with coarse roots biomass was developed across sites and treatments.

  12. INFLUENCIA DE LA MADERA JUVENIL DE PINO RADIATA SOBRE LAS PROPIEDADES MECÁNICAS DE TABLEROS OSB

    OpenAIRE

    PECHO,Robert; ANANIAS,Rubén A; BALLERINI,Aldo; CLOUTIER,Alain

    2004-01-01

    En este trabajo se estudia la influencia de la madera juvenil de pino radiata (Pinus radiata D. Don) sobre las propiedades mecánicas de tableros de hojuelas orientadas OSB. La madera para los ensayos es recogida de 10 árboles en pié de 26 años creciendo en plantaciones manejadas de la Octava región, Chile. La determinación de madera juvenil se realiza observando la variación radial de los anillos de crecimiento de la madera, usando un analizador de anillos en base a rayos X. Los detalles de l...

  13. Proteomic analysis of Pinus radiata needles: 2-DE map and protein identification by LC/MS/MS and substitution-tolerant database searching.

    Science.gov (United States)

    Valledor, Luis; Castillejo, Maria A; Lenz, Christof; Rodríguez, Roberto; Cañal, Maria J; Jorrín, Jesús

    2008-07-01

    Pinus radiata is one of the most economically important forest tree species, with a worldwide production of around 370 million m (3) of wood per year. Current selection of elite trees to be used in conservation and breeding programes requires the physiological and molecular characterization of available populations. To identify key proteins related to tree growth, productivity and responses to environmental factors, a proteomic approach is being utilized. In this paper, we present the first report of the 2-DE protein reference map of physiologically mature P. radiata needles, as a basis for subsequent differential expression proteomic studies related to growth, development, biomass production and responses to stresses. After TCA/acetone protein extraction of needle tissue, 549 +/- 21 well-resolved spots were detected in Coommassie-stained gels within the 5-8 pH and 10-100 kDa M(r) ranges. The analytical and biological variance determined for 450 spots were of 31 and 42%, respectively. After LC/MS/MS analysis of in-gel tryptic digested spots, proteins were identified by using the novel Paragon algorithm that tolerates amino acid substitution in the first-pass search. It allowed the confident identification of 115 out of the 150 protein spots subjected to MS, quite unusual high percentage for a poor sequence database, as is the case of P. radiata. Proteins were classified into 12 or 18 groups based on their corresponding cell component or biological process/pathway categories, respectively. Carbohydrate metabolism and photosynthetic enzymes predominate in the 2-DE protein profile of P. radiata needles.

  14. Productivity and cost of harvesting a stemwood biomass product from integrated cut-to-length harvest operations in Australian Pinus radiata plantations

    International Nuclear Information System (INIS)

    Walsh, D.; Strandgard, M.

    2014-01-01

    Significant quantities of woody biomass from the tops of trees and larger woody ‘waste’ pieces that fall outside existing sawlog and pulpwood specifications are left on site post final harvest in Australian radiata Pinus radiata (D. Don) (radiata pine) plantations. Woody biomass is a potential product for pulp making or energy generation. Commercial use of woody biomass from radiata pine plantations would add extra value to the Australian plantation estate through improved resource utilisation, and potentially reduced post-harvesting silvicultural costs. This study investigated the productivity and cost impact of the harvest and extraction to roadside of woody biomass in an integrated harvest operation in a typical Australian two machine (harvester/processor and forwarder), cut-to-length, clearfall operation in a mature, thinned radiata pine plantation. The harvest operation yielded 23 GMt/ha (5% of the total yield) of woody biomass (known as ‘fibreplus’), 443 GMt/ha of sawlogs and 28 GMt/ha of pulpwood. The mean quantity of biomass left on site was 128 GMt/ha, mainly consisting of branches and needles, sufficient to minimise nutrient loss and protect the soil from erosion. Woodchips derived from the fibreplus product were suitable for kraft pulp making, (when blended in small amounts with clean de-barked roundwood woodchips), and for energy generation. The method trialed with the fibreplus product being produced did not impact harvesting and processing productivity and costs, but extraction was 14% less productive. Through analysis of the productivities of each phase and development of a cost model the harvest and extraction of the fibreplus product was estimated to increase total unit costs by ∼4.9%. - Highlights: • Study of the productivity and cost impact of producing a woody biomass product. • We compared two scenarios – harvesting with and without the biomass product. • An additional 23 GMt/ha (5% of the total yield) of woody biomass

  15. Sub-Compartment Variation in Tree Height, Stem Diameter and Stocking in a Pinus radiata D. Don Plantation Examined Using Airborne LiDAR Data

    Directory of Open Access Journals (Sweden)

    Hanieh Saremi

    2014-08-01

    Full Text Available Better information regarding the spatial variability of height, Diameter at Breast Height (DBH and stocking could improve inventory estimates at the operational Planning Unit since these parameters are used extensively in allometric equations, including stem volume, biomass and carbon calculations. In this study, the influence of stand stocking on height and DBH of two even aged radiata pine (Pinus radiata D. Don stands were investigated using airborne Light Detection and Ranging (LiDAR data at a study site in New South Wales, Australia. Both stands were characterized by irregular stocking due to patchy establishment and self-thinning in the absence of any silvicultural thinning events. For the purpose of this study, a total of 34 plots from a 34 year old site and 43 plots from a nine year old site were established, from which a total of 447 trees were sampled. Within these plots, DBH and height measurements were measured and their relationships with stocking were evaluated. LiDAR was used for height estimation as well as stem counts in fixed plots (stocking. The results showed a significant relationship between stem DBH and stocking. At both locations, trees with larger diameters were found on lower stocking sites. Height values were also significantly correlated with stocking, with taller trees associated with high stocking. These results were further verified of additional tree samples, with independent field surveys for DBH and LiDAR-derived metrics for height analysis. This study confirmed the relationship between P. radiata tree heights and stem diameter with stocking and demonstrated the capacity of LiDAR to capture sub-compartment variation in these tree-level attributes.

  16. Seasonal Terpene Variation in Needles of Pinus radiata (Pinales: Pinaceae) Trees Attacked by Tomicus piniperda (Coleoptera: Scolytinae) and the Effect of Limonene on Beetle Aggregation.

    Science.gov (United States)

    Romón, Pedro; Aparicio, Domitila; Palacios, Francisco; Iturrondobeitia, Juan Carlos; Hance, Thierry; Goldarazena, Arturo

    2017-09-01

    Concentrations of four monoterpenes were determined in needles of Pinus radiata (D.Don) (Pinales: Pinaceae) trees that were attacked or nonattacked by Tomicus piniperda (L.) (Coleoptera: Scolytinae). Compounds were identified and quantified by gas chromatography-mass spectrometry. The mean ambient temperature was obtained using climate-recording data loggers. The effect of limonene on field aggregation was also evaluated at three limonene release rates using Lindgren attractant-baited traps and trap logs. Attacked trees produced less α-pinene in March, July, and November than nonattacked trees, less β-pinene in July and November, and less limonene from May to November. Limonene reduced the attraction of T. piniperda to attractant-baited traps and trap logs. Results were linked to better responses to high temperatures, with respect to terpene contents, by the nonattacked trees after the spring attack. © The Author 2017. Published by Oxford University Press on behalf of Entomological Society of America.

  17. No impact of transgenic nptII-leafy Pinus radiata (Pinales: Pinaceae) on Pseudocoremia suavis (Lepidoptera: Geometridae) or its endoparasitoid Meteorus pulchricornis (Hymenoptera: Braconidae).

    Science.gov (United States)

    Burgess, E P J; Barraclough, E I; Kean, A M; Walter, C; Malone, L A

    2011-10-01

    To investigate the biosafety to insects of transgenic Pinus radiata D. Don containing the antibiotic resistance marker gene nptII and the reproductive control gene leafy, bioassays were conducted with an endemic lepidopteran pest of New Zealand plantation pine forests and a hymenopteran endoparasitoid. Larvae of the common forest looper, Pseudocoremia suavis (Butler), were fed from hatching on P. radiata needles from either one of two nptII-leafy transgenic clones, or an isogenic unmodified control line. For both unparasitized P. suavis and those parasitized by Meteorus pulchricornis (Wesmael), consuming transgenic versus control pine had no impact on larval growth rate or mass at any age, larval duration, survival, pupation or successful emergence as an adult. Total larval duration was 1 d (3%) longer in larvae fed nptII-2 than nptII-1, but this difference was considered trivial and neither differed from the control. In unparasitized P. suavis larvae, pine type consumed did not affect rate of pupation or adult emergence, pupal mass, or pupal duration. Pine type had no effect on the duration or survival of M. pulchricornis larval or pupal stages, mass of cocoons, stage at which they died, adult emergence, or fecundity. Parasitism by M. pulchricornis reduced P. suavis larval growth rate, increased the duration of the third larval stadium, and resulted in the death of all host larvae before pupation. The lack of impact of an exclusive diet of nptII-leafy transgenic pines on the life history of P. suavis and M. pulchricornis suggests that transgenic plantation pines expressing nptII are unlikely to affect insect populations in the field.

  18. Experimental determination of the effect of temperature and humidity on the development of colour in Pinus radiata

    Directory of Open Access Journals (Sweden)

    M. McCurdy

    2005-06-01

    Full Text Available Experiments were undertaken to determine the effects of drying conditions (temperature and humidity on the development of kiln brown stain in radiata pine during drying. Eight schedules were tested with temperatures ranging from 50°C to 120°C and relative humidity from 14% to 67%. The variables measured were moisture content, color expressed using the CIELab color space, and nitrogen content. The experiments have shown that the kiln brown stain is influenced by drying temperature and drying time. The recommendation is therefore that low-temperature and low-humidity schedules be developed for controlling color development.

  19. Variations in dark respiration and mitochondrial numbers within needles of Pinus radiata grown in ambient or elevated CO2 partial pressure

    International Nuclear Information System (INIS)

    Griffin, K. L.; Anderson, O. R.; Tissue, D. T.; Turnbull, M. H.; Whitehead, D.

    2004-01-01

    An experiment involving comparison of within-leaf variations in cell size, mitochondrial numbers and dark respiration in the most recently expanded tip, the mid-section and the base of needles of Pinus radiata grown for four years at ambient and elevated carbon dioxide partial pressure, is described. Results showed variation in mitochondrial numbers and respiration along the length of the needle, with the highest number of mitochondria per unit cytoplasm and the highest rate of respiration per unit leaf area at the base of the needle. Elevated carbon dioxide pressure caused the number of mitochondria per unit cytoplasm to double regardless of location (tip, basal or mid sections). Under these conditions, greatest mitochondrial density was observed at the tip. The mean size of mitochondria was not affected by either growth at elevated carbon dioxide pressure or by position on the needle. Respiration per unit leaf area at elevated carbon dioxide pressure was highest at the tip of needles, decreasing towards the middle and basal sections. The observed data supports the hypothesis that the highest number of mitochondria per unit area of cytoplasm occurs at the base of the needle, but does not support the hypothesis that the lowest rate of respiration also occurs at the base. It is suggested that the relationship that determines the association between structure and function in these needles is more complex than previously thought. 33 refs., 4 tabs., 1 fig

  20. Effect of liming and organic and inorganic fertilization on soil carbon sequestered in macro-and microaggregates in a 17-year old Pinus radiata silvopastoral system.

    Science.gov (United States)

    Mosquera-Losada, M R; Rigueiro-Rodríguez, A; Ferreiro-Domínguez, N

    2015-03-01

    Agroforestry systems have been recognized as a potential greenhouse gas mitigation strategy under the Kyoto Protocol because of their ability to absorb carbon dioxide from the atmosphere and store carbon mainly in the soil. Soil particle size and land management practices are known to have a considerable influence on carbon storage in soils. This study evaluated changes in soil chemical and physical properties, and quantified and compared the amount of C stored in the bulk soil and in three different soil fractions (250-2000, 53-250 and silvopastoral system located on an acidic forest soil under Pinus radiata D. Don. Areas of this system were subjected ten years ago to one of nine fertilization treatments: three different doses of sewage sludge or no fertilization, all with or without the addition of lime, and mineral fertilizer with no liming. Seventeen years after reforestation and seven years after canopy closure, strong gradients with soil depth were found regarding soil bulk density, pH and carbon storage. Intense soil management (high doses of sewage sludge and liming) generally reduced soil carbon storage, mainly in coarse aggregates, but this could be compensated by the increase in tree and pasture development observed in soils subject to intermediate sewage sludge doses. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. Extração e caracterização de hemiceluloses de Pinus radiata e sua viabilidade para a produção de bioetanol

    Directory of Open Access Journals (Sweden)

    Pablo Reyes

    2013-02-01

    Full Text Available As galactoglucomananas são as principais frações de hemiceluloses presentes nas madeiras moles e contêm, principalmente, as hexoses galactose, glicose e manose. O isolamento eficiente e seletivo dessas hemiceluloses é um obstáculo crítico a superar para sua utilização. Os objetivos deste trabalho foram extrair e caracterizar soluções aquosas ácidas e neutras de hemiceluloses de cavacos de madeira de Pinus radiata, bem como avaliar sua viabilidade para a produção de bioetanol. As hemiceluloses em P. radiata representam 26 g/100 g de madeira (base seca, e as hexoses são responsáveis por aproximadamente 64% dessa quantidade. De acordo com as diferentes condições de extração, cerca de 50% da fração hemicelulósica foi solubilizada e recuperada depois de uma precipitação com etanol. As frações recuperadas de hemiceluloses estavam na forma de oligômeros com peso molecular médio (Mw variando entre 4x10³ e 4x10(5 g/mol. Os oligômeros hemicelulósicos foram hidrolisados com ácido sulfúrico diluído e os hidrolisados concentrados até aproximadamente 70 g/L hexosas e fermentados pela levedura Saccharomyces cerevisiae. Os resultados de fermentação indicaram que os açúcares obtidos dos extratos ácidos e neutros foram fermentados com rendimentos máximos de etanol de 63% e 54% (22 g/L e 19 g/L, respectivamente. A conversão de hemiceluloses da madeira em etanol é viável, porém seu baixo rendimento faz que o processo não seja economicamente atrativo, razão por que melhorias no processo ou usos alternativos das hemiceluloses devem ser avaliados.

  2. Extração e caracterização de hemiceluloses de Pinus radiata e sua viabilidade para a produção de bioetanol Extraction and characterization of hemicelluloses from Pinus radiata and its feasibility for bioethanol production

    Directory of Open Access Journals (Sweden)

    Pablo Reyes

    2013-02-01

    Full Text Available As galactoglucomananas são as principais frações de hemiceluloses presentes nas madeiras moles e contêm, principalmente, as hexoses galactose, glicose e manose. O isolamento eficiente e seletivo dessas hemiceluloses é um obstáculo crítico a superar para sua utilização. Os objetivos deste trabalho foram extrair e caracterizar soluções aquosas ácidas e neutras de hemiceluloses de cavacos de madeira de Pinus radiata, bem como avaliar sua viabilidade para a produção de bioetanol. As hemiceluloses em P. radiata representam 26 g/100 g de madeira (base seca, e as hexoses são responsáveis por aproximadamente 64% dessa quantidade. De acordo com as diferentes condições de extração, cerca de 50% da fração hemicelulósica foi solubilizada e recuperada depois de uma precipitação com etanol. As frações recuperadas de hemiceluloses estavam na forma de oligômeros com peso molecular médio (Mw variando entre 4x10³ e 4x10(5 g/mol. Os oligômeros hemicelulósicos foram hidrolisados com ácido sulfúrico diluído e os hidrolisados concentrados até aproximadamente 70 g/L hexosas e fermentados pela levedura Saccharomyces cerevisiae. Os resultados de fermentação indicaram que os açúcares obtidos dos extratos ácidos e neutros foram fermentados com rendimentos máximos de etanol de 63% e 54% (22 g/L e 19 g/L, respectivamente. A conversão de hemiceluloses da madeira em etanol é viável, porém seu baixo rendimento faz que o processo não seja economicamente atrativo, razão por que melhorias no processo ou usos alternativos das hemiceluloses devem ser avaliados.Galactoglucomannans are the major hemicellulosic fraction present in softwoods, and is mainly composed by the hexoses: galactose, glucose and mannose. The efficient and selective isolation of these hemicelluloses is regarded as one of the most critical obstacles to overcome for their utilization. The objective of the current work was to extract and characterize acidic and

  3. Investigation on the variation of annual ring thicknesses and word densities in South African Pinus radiata stands under the influence of climate and different thinning measures by means of radiodensitometry

    International Nuclear Information System (INIS)

    Bues, C.T.

    1984-01-01

    The present work investigates three phenomena of growth biology in Pinus radiata stands: In the first investigation project: the thickness of the annual rings in relation to climate factors; In the second project: the variability of annual ring thickness and wood density as a function of different thinning measures; In the third project the variability of wood density within a Pinus radiate stand is analyzed. To determine the thickness of the annual rings and the wood density values within the annual rings, the method of radiodensitometry, developed during the last decade, is adopted: In the first investigation project the thicknesses of 800 annual rings are determined, in the second project 3808 annual ring structures are investigated, and in the third project 2000 annual rings are evaluated for their density characteristics. (orig./MG) [de

  4. Flight Dynamics and Abundance of Ips sexdentatus (Coleoptera: Curculionidae: Scolytinae in Different Sawmills from Northern Spain: Differences between Local Pinus radiata (Pinales: Pinaceae and Southern France Incoming P. pinaster Timber

    Directory of Open Access Journals (Sweden)

    Sergio López

    2012-01-01

    Full Text Available In January 2009, the windstorm “Klaus” struck the southern part of France, affecting 37.9 million m3 of maritime pine Pinus pinaster Aiton (Pinales: Pinaceae. This breeding plant material favored the outbreak of Ips sexdentatus (Börner (Coleoptera: Curculionidae: Scolytinae. As much of this timber is imported to the Basque Country (northern Spain, a potential risk to conifer stands is generated, due to the emergence of the incoming beetles. Thus, flight dynamics and beetle abundance were compared in different sawmills, according to the timber species (either local P. radiata D. Don or imported P. pinaster. A maximum flight peak of I. sexdentatus was observed in mid-June in P. pinaster importing sawmills, whereas a second lighter peak occurred in September. In contrast, only a maximum peak in mid-June was observed in P. radiata inhabiting beetles, being significantly smaller than in local P. pinaster trading sawmills. In addition, significant differences were found between imported P. pinaster and P. radiata regarding the number of insects beneath the bark. The development of IPM strategies for controlling I. sexdentatus populations is recommended, due to the insect abundance found in P. pinaster imported timber.

  5. Comunidad ectomicorrícica en una cronosecuencia de Pinus radiata (Pinophyta: Pinaceae de la zona de transición climática mediterráneo-templada de Chile central The ectomycorrhizal community in a chronosequence of Pinus radiata (Pinophyta: Pinaceae of the transitional Mediterranean-temperate climatic zone of central Chile

    Directory of Open Access Journals (Sweden)

    YUSSI M PALACIOS

    2012-03-01

    Full Text Available En ecosistemas naturales y plantaciones, las coníferas establecen asociaciones mutualistas con una comunidad diversa de hongos micorrícicos. El estudio de este tema en Sudamérica es aún incipiente, y no existen antecedentes sobre la dinámica temporal de esta comunidad, y menos de sus potenciales causas, a pesar de su importancia para un país forestal como Chile. En el presente trabajo se evaluó la dinámica de la comunidad ectomicorrícica, identificando y cuantificando los hongos formadores de esta asociación en raíces finas de Pinus radiata en plantaciones de 3, 10 y 20 años. Los resultados confirman que la comunidad ectomicorrícica de P. radiata cambia con la edad de los árboles, la cual difiere más bien en el patrón de dominancia que en la riqueza de especies, separándose un primer grupo de árboles de 3 y 10 años de un segundo grupo de 20 años. Un total de once morfotipos de micorrizas fueron diferenciados. Cuatro de ellos, identificados como Hebeloma crustuliniforme, Inocybe sp., Russula sardonia y Pinirhiza spinulosa, fueron los más abundantes (77, 29, 78 y 8 % respectivamente, mientras las otras se encontraron colonizando menos de 100 puntas de raíz (In natural forest ecosystems and plantations, most trees live in mutualistic association with mycorrhizal fungi. Studies of this association in South America are still scarce, especially when referring to the causes of temporal dynamics of this symbiotic community, despite its importance in countries with a thriving forestry industry like Chile. This study evaluates the dynamics of the ectomycorrhizal community of Pinus radiata stands of 3, 10 and 20 years of age, identifying and quantifying the most common fungal colonizers of fine roots in each age class. The results confirm that the mycobiont community changes with host tree age but that age classes differ in dominance patterns rather than in species richness, with the three- and ten-year-old tree cohorts forming a

  6. Mineralización del nitrógeno, carbono y actividad enzimática del suelo en un bosque de Nothofagus obliqua (Mirb Oerst y una plantación de Pinus radiata D. Don. del centro-sur de Chile Nitrogen and carbon mineralization and enzyme activity in soils of Nothofagus obliqua (Mirb Oerst stands and Pinus radiata D. Don plantation in south-central Chile

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    YESSICA RIVAS

    2009-03-01

    Full Text Available En Chile, el establecimiento de plantaciones comerciales de rápido crecimiento ha sido sostenido en las últimas décadas mediante la sustitución de bosques nativos y conversión de suelos agrícolas. Pinus radiata D. Don es la principal especie productiva, debido a su crecimiento acelerado y adaptabilidad al clima y los suelos. En el presente estudio se plantea que la actividad biológica del suelo es variable a través del año, en respuesta a variaciones de precipitación, temperatura y contenido de humedad de suelo y que el cambio de uso de suelo desde un bosque templado de Nothofagus obliqua (Mirb Oerst a una plantación con coniferas exóticas, modifica la química del suelo y consecuentemente los procesos de N-min, C-min y la actividad biológica del suelo. Esta hipótesis fue examinada en un bosque de N. obliqua y una plantación de P. radiata del centro-sur de Chile (40°07' S, 72° O. Se evaluó mensualmente la tasa mineralización de nitrógeno (N-min, cabono (C-min y la actividad enzimática potencial del suelo (ureasa, proteasa e hidrólisis de la fluoresceína diacetato entre septiembre 2003 y mayo 2005. Los resultados demuestran que los niveles de las variables de actividad biológica del suelo fueron significativamente diferentes entre las parcelas de bosque y plantación (Lambda de Wilk = 0,022; F 5,80 = 733; P In Chile, commercial forests plantations have increased during the last decades due in part to replacement of native forests and conversion of agricultural soils. Pinus radiata D. Don has been the main tree planted, due to its rapid growth and adaptability. In the present study we proposed that biological activity varies along the year due to changes of precipitation, temperature and soil water content and mainly because the conversion of native forest to exotic P. radiata plantations alters the soil chemistry, N and C mineralization and the potential enzymatic activity in these soils. This hypothesis was examined in a

  7. Biomass partitioning and leaf area of Pinus radiata trees subjected to silvopastoral and conventional forestry in the VI region, Chile Distribución de biomasa y área foliar en árboles de Pinus radiata sometidos a manejo silvopastoral y convencional en la VI región, Chile

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    ROLANDO RODRÍGUEZ

    2003-09-01

    Full Text Available The effects of silvicultural regimes on leaf area and biomass distribution were analyzed in 16-year old Pinus radiata trees growing in the semiarid zone of Chile. Three stands with different silvopastoral management were compared with a conventionally managed stand. Data were obtained through destructive sampling of 36 trees and analyzed by MANOVA and regression models of ANCOVA. Results show that the management regime affects the leaf area. Specific leaf area was affected by both silvicultural regime and crown position. Total biomass per tree under the silvopastoral regime was 2.1 to 2.5 times larger than in the conventional forestry regime. However, aboveground biomass partitioning was neither affected by the silvicultural regime nor by the schemes of silvopastoral management. The most important allometric change was in fine root biomass, which was greater under the conventional forestry regime than in the silvopastoral one. Fine root biomass increases with a regular distribution of the plants in the field, and decreases with the clumping of trees. Similarly, the fine root biomass decreases with fertilization. Both plantation design and fertilization regimes explain the changes in the fine root biomass to components of the crown. However, crown structure influences the magnitude of these changes.Se analizaron los efectos del régimen silvícola en el área foliar y distribución de biomasa en árboles de Pinus radiata de 16 años, creciendo en la zona semiárida de Chile. Para ello se compararon tres rodales con manejo silvopastoral con uno manejado en forma tradicional. Los datos se obtuvieron mediante muestreo destructivo de 36 árboles y se analizaron mediante MANOVA y regresión en modelos de ANCOVA. Los resultados permiten concluir que el régimen de manejo afectó el área foliar. El área foliar específica fue afectada por el régimen silvícola y su posición en la copa. La biomasa total por árbol con régimen silvopastoral es 2,1 a

  8. Defect core detection in radiata pine logs

    International Nuclear Information System (INIS)

    Wallace, G.

    1993-01-01

    Internal defect cores in Pinus radiata logs arise primarily from the practice in New Zealand of pruning trees to increase the amount of clear wood. Realising the benefits of this practice when milling the logs is hampered by the lack of a practical method for detecting the defect cores. This report attempts to establish industry requirements for detections and examine techniques which may be suitable. Some trials of a novel technique are described. (author) 19 refs.; 11 figs

  9. Influencia del deterioro incipiente producido por el hongo de pudrición parda Serpula lacrymans, sobre las propiedades mecánicas de compresión normal y paralela a la fibra en madera de Pinus radiata D. Don

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    Pereira, A.

    2011-03-01

    Full Text Available Several buildings and structures of wood are attacked by rot fungi, leading in some cases, slight weight losses that result in reductions in the strength properties of the material. In this work was evaluated the influence of early rot decay in wood, in mechanical properties of normal and parallel compression. For this, were inoculated samples of Pinus radiata D. Don with brown rot fungi Serpula Lacrymans. These samples were tested in three differents periods of fungal exposure. The results identified a significant decrease in percentage of the properties under study.Diversas edificaciones y estructuras construidas en madera son atacadas por hongos de pudrición, provocando en algunos casos, leves pérdidas de peso que se traducen en disminución en las propiedades resistentes del material. En este trabajo se evaluó la influencia de estados incipientes de pudrición en madera, en las propiedades mecánicas de compresión paralela y compresión normal. Para esto, se inocularon probetas de Pinus radiata D. Don. con el hongo de pudrición parda Serpula lacrymans, las cuales fueron posteriormente ensayadas mecánicamente en tres diferentes períodos de exposición fúngica. Los resultados obtenidos determinaron una importante disminución porcentual de las propiedades bajo estudio.

  10. Estudio del efecto de la dirección de medición y contenido de humedad en la velocidad de ultrasonido en madera de Pinus radiata D. Don a través de análisis de varianza

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    Rodrigo Ortíz Mansilla

    2009-09-01

    Full Text Available Para analizar la técnica de ultrasonido como una alternativa en la evaluación del estado estructural de diferentes edificaciones construidas en madera, se estudió el efecto e importancia relativa de la dirección de medición y el contenido de humedad, so- bre la velocidad de transmisión en este material. Para esto, se aplicaron pulsos longitudinales ultrasónicos de 1 Mhz sobre pro- betas de madera de Pinus radiata D. Don, bajo un procedimiento experimental que consideró la utilización de un diseño fac- torial 22 completamente aleatorizado. Como resultado del análisis de varianza, se determinó que dentro de los rangos de con- tenido de humedad y dirección de medición estudiados, este último se presenta como factor significativo, explicando el 95% de la variabilidad total observada. De hecho, la variación del contenido de humedad entre 0 y 15%, conlleva a valores de veloci- dad de propagación promedio de 1.644 m/s y 2.130 m/s para las direcciones tangencial y radial, respectivamente. Los resulta- dos de este trabajo permiten concluir que al momento de realizar una evaluación mediante ultrasonido en estructuras en servi- cio, principalmente se debe considerar la dirección de medición como un factor determinante en el estudio; sin embargo, la e- fectividad de aplicación de esta técnica requiere de otros trabajos complementarios que consideren la propagación en planos mixtos de la madera. Este estudio se llevará a cabo próximamente.

  11. DETERMINACIÓN DE MEDIOS DE CULTIVO Y PH PARA LA MASIFICACIÓN in vitro DE CEPAS DE Suillus luteus Aubl. ASOCIADAS A Pinus radiata D. Don Y Scleroderma citrinum Pers. ASOCIADAS A Eucalyptus globulus Labill. DE LA REGIÓN DEL BIOBÍO, CHILE

    Directory of Open Access Journals (Sweden)

    Marta González

    2015-02-01

    Full Text Available El uso de agentes biológicos en la producción en vivero ha aflorado como un elemento que permite no sólo mejorar la calidad morfológica y fisiológica de las plantas, sino que también posibilitar un mayor éxito en el ámbito silvicultural de una plantación. Estos agentes biológicos requieren de un ambiente óptimo para crecer, asociarse y reproducirse. El objetivo de este estudio fue determinar la dependencia del pH y el medio de cultivo en el crecimiento in vitro de distintas cepas de Suillus luteus y Scleroderma citrinum asociadas a Pinus radiata y Eucalyptus globulus, respectivamente. El estudio se realizó en condiciones controladas de temperatura y humedad, disponiendo los inóculos en placas Petri con diferentes medios cultivo, evaluando parámetros de crecimiento y biomasa a los 38 días para S. luteus y 105 días para S. citrinum. Los resultados indican que tanto el medio de cultivo, el pH del medio, así como las cepas de cada especie estudiada, son determinantes en las respuestas de crecimiento de los hongos ectomicorrícicos in vitro evaluados. Las cepas de S. luteus se desarrollaron adecuadamente en un medio de cultivo con abundancia de nutrientes (BAF, MMN como en baja presencia de ellos (EMA, y con pH más bien ácido (4,8 y 5,8. Por otro lado, las cepas de S. citrinum presentaron, para los mismos ambientes, un desarrollo inferior y lento, no obstante, la cepa Sc8 se reprodujo de forma óptima y rápida bajo un medio de cultivo BAF y con un pH moderadamente ácido de 5,8.

  12. EFFECTIVENESS OF A SUPERFICIAL TREATMENT USING BIFENTHRIN TO PROTECT RADIATA PINE FRAMING FROM DAMAGE BY SUBTERRANEAN AND DRYWOOD TERMITES IN INDONESIA

    OpenAIRE

    Paimin Sukartana; Jim W. Creffield; Agus Ismanto; Neo E. Lelana; Rusti Rushelia

    2010-01-01

    Various experimental testing procedures were undertaken in Indonesia to determine the effectiveness of a patented superficial (envelope) treatment using bifenthrin to protect radiata pine framing material from damage by two species of subterranean termites (Macrotermes gilvus and Coptotermes curvignathus) and one species of drywood termite (Cryptotermes cynocephalus). Lengths of framing material (Pinus radiata sapwood) were commercially treated to the targeted retention of 0.02% m/m of bifent...

  13. Equilibrium moisture content of radiata pine at elevated temperature and pressure reveals measurement challenges

    DEFF Research Database (Denmark)

    Pearson, Hamish; Gabbitas, Brian; Ormarsson, Sigurdur

    2012-01-01

    moisture contents were attributed to condensation of liquid water on the specimen with subsequent evaporation at a rate that was too slow for the moisture content to reach equilibrium before it was measured. Reliable EMC data at elevated temperatures require (1) tight process control of experimental......Relatively few studies have been performed on the equilibrium moisture content (EMC) of wood under conditions of elevated temperature and pressure. Eight studies indicated that EMC near saturation decreased between 100 and 150 °C, whilst five studies indicated that EMC increased. The aim...... of this study was to identify the likely source of the disagreement using radiata pine (Pinus radiata D. Don) sapwood which was conditioned to a moisture content of around 3 % and then exposed for 1 h at 150 °C and relative humidities of either 50, 70 or 90 %. Mean values of EMC, obtained through in situ...

  14. Datalogger usando nios ii

    OpenAIRE

    Campoverde Rugel, Luis Enrique; Velásquez Vargas, Washington Adrián; Ponguillo, Ronald

    2013-01-01

    El presente proyecto consiste en la implementación de un Datalogger utilizando el microprocesador NIOS II el cual fue embebido en el FPGA CYCLONE II que se encuentra integrada en la tarjeta de desarrollo ALTERA DE2, el cual obtiene datos de distintos sensores y los almacena en una tarjeta SD Card. Para la realización del proyecto se aplican cuatro etapas. La primera etapa está basada en obtener los datos mediante el uso de sensores y la transmisión usando un PIC, la siguiente etapa se basa...

  15. The potential of breeding for enhanced inducibility in Pinus pinaster and Pinus radiata

    Science.gov (United States)

    Rafael Zas; Alejandro Solla; Xoaquin Moreira; Luis Sampedro

    2012-01-01

    Most resistance mechanisms against pests and pathogens in pine trees involve the production of chemical defenses. These defenses are not cost free and the production of secondary metabolisms is generally inversely related with other plant fitness correlates, such as growth. The existence of these negative genetic correlations imposes an important obstacle for breeding...

  16. Physiological response to drought in radiata pine: phytohormone implication at leaf level.

    Science.gov (United States)

    De Diego, N; Pérez-Alfocea, F; Cantero, E; Lacuesta, M; Moncaleán, P

    2012-04-01

    Pinus radiata D. Don is one of the most abundant species in the north of Spain. Knowledge of drought response mechanisms is essential to guarantee plantation survival under reduced water supply as predicted in the future. Tolerance mechanisms are being studied in breeding programs, because information on such mechanisms can be used for genotype selection. In this paper, we analyze the changes of leaf water potential, hydraulic conductance (K(leaf)), stomatal conductance and phytohormones under drought in P. radiata breeds (O1, O2, O3, O4, O5 and O6) from different climatology areas, hypothesizing that they could show variable drought tolerance. As a primary signal, drought decreased cytokinin (zeatin and zeatin riboside-Z + ZR) levels in needles parallel to K(leaf) and gas exchange. When Z + ZR decreased by 65%, indole-3-acetic acid (IAA) and abscisic acid (ABA) accumulation started as a second signal and increments were higher for IAA than for ABA. When plants decreased by 80%, Z + ZR and K(leaf) doubled their ABA and IAA levels, the photosystem II yield decreased and the electrolyte leakage increased. At the end of the drought period, less tolerant breeds increased IAA over 10-fold compared with controls. External damage also induced jasmonic acid accumulation in all breeds except in O5 (P. radiata var. radiata × var. cedrosensis), which accumulated salicylic acid as a defense mechanism. After rewatering, only the most tolerant plants recovered their K(leaf,) perhaps due to an IAA decrease and 1-aminocyclopropane-1-carboxylic acid maintenance. From all phytohormones, IAA was the most representative 'water deficit signal' in P. radiata.

  17. Identification case of evidence in timber tracing of Pinus radiate, using high-resolution melting (HRM) analysis.

    Science.gov (United States)

    Solano, Jaime; Anabalón, Leonardo; Encina, Francisco

    2016-03-01

    Fast, accurate detection of plant species and their hybrids using molecular tools will facilitate assessment and monitoring of timber tracing evidence. In this study the origin of unknown pine samples is determined for a case of timber theft in the region of Araucania southern Chile. We evaluate the utility of the trnL marker region for species identification applied to pine wood based on High Resolution Melting. This efficient tracing methods can be incorporated into forestry applications such as certification of origin. The object of this work was genotype identification using high-resolution melting (HRM) and trnL approaches for Pinus radiata (Don) in timber tracing evidence. Our results indicate that trnL is a very sensitive marker for delimiting species and HRM analysis was used successfully for genotyping Pinus samples for timber tracing purposes. Genotyping samples by HRM analysis with the trnL1 approach allowed us to differentiate two wood samples from the Pinaceae family: Pinus radiata (Don) and Pseudotsuga menziesii (Mirb.) Franco. The same approach with Pinus trnL wood was not able to discriminate between samples of Pinus radiata, indicating that the samples were genetically indistinguishable, possibly because they have the same genotype at this locus. Timber tracing with HRM analysis is expected to contribute to future forest certification schemes, control of illegal trading, and molecular traceability of Pinus spp. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  18. Characterization of moisture in acetylated and propionylated radiata pine using low-field nuclear magnetic resonance (LFNMR) relaxometry

    DEFF Research Database (Denmark)

    Beck, Greeley; Thybring, Emil Engelund; Thygesen, Lisbeth Garbrecht

    2018-01-01

    . A possible explanation is the counteracting effects of decreased hydrophilicity and reduced moisture content (MC) of these water populations at higher levels of acetylation. The evaluation of propionylation on WCW T2 data was complicated by peak splitting in the relaxation spectrum. Constant T2 values......Moisture in radiata pine (Pinus radiata D. Don) earlywood (EW), which was acetylated or propionylated to various degrees, was measured by low-field nuclear magnetic resonance (LFNMR) relaxometry. Spin-spin relaxation times (T2) were determined for fully saturated samples at 22 and -18°C. T2 values...... for EW lumen water increased with increasing acetylation weight percentage gain (WPG), perhaps caused by the less hydrophilic acetylated wood (AcW) surface. Cell wall water (WCW) and the water in pits and small voids also showed increasing T2 values as a function of WPG but with a weaker tendency...

  19. Male sterile mutant in Vigna radiata

    International Nuclear Information System (INIS)

    Pande, Kalpana; Raghuvanshi, S.S.

    1987-01-01

    Single and combined treatment of γ-rays and 0.25 per cent EMS were tried on Vigna radiata variety K851. A male sterile mutant was isolated in M 2 generation. Experiments indicated male sterility to be recessive and monogenic in nature. 6 figures. (author)

  20. Bioactive phenolic acids from Scorzonera radiata Fisch.

    Directory of Open Access Journals (Sweden)

    N Tsevegsuren

    2014-09-01

    Full Text Available Chromatographic separation of the crude extract obtained from the aerial parts of the Mongolian medicinal plant Scorzonera radiata yielded five new dihydrostilbenes [4], two new flavonoids, one new quinic acid derivative, as well as twenty known compounds including eight quinic acid derivatives, four flavonoids, two coumarins, five simple benzoic acids, and one monoterpene glycoside. We present here results on isolation and structural identification some active phenolic compounds from the Scorzonera radiata - eight quinic acid derivatives (quinic acid, 4,5-dicaffeoylquinic acid, 4,5-dicaffeoyl-epi-quinic acid, 3,5-dicaffeoylquinic acid, 3,5-dicaffeoyl-epi-quinic acid, chlorogenic acid, 5-p-coumaroylquinic acid (trans, 5-p-coumaroylquinic acid (cis. Quinic acid derivatives exhibited antioxidative activity.DOI: http://dx.doi.org/10.5564/mjc.v12i0.177 Mongolian Journal of Chemistry Vol.12 2011: 78-84

  1. Comparative nutritional analysis between Vigna radiata and Vigna ...

    African Journals Online (AJOL)

    Vigna radiata (mung bean) and Vigna mungo (mash bean) of the family Fabaceae are among staple food in Pakistan. The experiments were conducted on these beans to determine the proximate composition such as moisture, ash, fibre, fat and protein content. The protein isolates from V. radiata and V. mungo was ...

  2. Metabolites and hormones are involved in the intraspecific variability of drought hardening in radiata pine.

    Science.gov (United States)

    De Diego, N; Saiz-Fernández, I; Rodríguez, J L; Pérez-Alfocea, P; Sampedro, M C; Barrio, R J; Lacuesta, M; Moncaleán, P

    2015-09-01

    Studies of metabolic and physiological bases of plant tolerance and hardening against drought are essential to improve genetic breeding programs, especially in productive species such as Pinus radiata. The exposure to different drought cycles is a highly effective tool that improves plant conditioning, but limited information is available about the mechanisms that modulate this process. To clarify this issue, six P. radiata breeds with well-known differences in drought tolerance were analyzed after two consecutive drought cycles. Survival rate, concentration of several metabolites such as free soluble amino acids and polyamines, and main plant hormones varied between them after drought hardening, while relative growth ratio and water potential at both predawn and dawn did not. Hardening induced a strong increase in total soluble amino acids in all breeds, accumulating mainly those implicated in the glutamate metabolism (GM), especially L-proline, in the most tolerant breeds. Other amino acids from GM such as γ-aminobutyric acid (GABA) and L-arginine (Arg) were also strongly increased. GABA pathway could improve the response against drought, whereas Arg acts as precursor for the synthesis of spermidine. This polyamine showed a positive relationship with the survival capacity, probably due to its role as antioxidant under stress conditions. Finally, drought hardening also induced changes in phytohormone content, showing each breed a different profile. Although all of them accumulated indole-3-acetic acid and jasmonic acid and reduced zeatin content in needles, significant differences were observed regarding abscisic acid, salicylic acid and mainly zeatin riboside. These results confirm that hardening is not only species-dependent but also an intraspecific processes controlled through metabolite changes. Copyright © 2015 Elsevier GmbH. All rights reserved.

  3. Ecological impacts of long-term application of biosolids to a radiata pine plantation

    International Nuclear Information System (INIS)

    Xue, Jianming; Kimberley, Mark O.; Ross, Craig; Gielen, Gerty; Tremblay, Louis A.; Champeau, Olivier; Horswell, Jacqui; Wang, Hailong

    2015-01-01

    Assessment of the ecological impact of applying biosolids is important for determining both the risks and benefits. This study investigated the impact on soil physical, chemical and biological properties, tree nutrition and growth of long-term biosolids applications to a radiata pine (Pinus radiata D. Don) plantation growing on a Sandy Raw Soil in New Zealand. Biosolids were applied to the trial site every 3 years from tree age 6 to 19 years at three application rates: 0 (Control), 300 (Standard) and 600 (High) kg nitrogen (N) ha −1 , equivalent to 0, 3 and 6 Mg ha −1 of dry biosolids, respectively. Tree nutrition status and growth have been monitored annually. Soil samples were collected 13 years after the first biosolids application to assess the soil properties and functioning. Both the Standard and High biosolids treatments significantly increased soil (0–50 cm depth) total carbon (C), N, and phosphorus (P), Olsen P and cation exchange capacity (CEC), reduced soil pH, but had no significant effects on soil (0–20 cm depth) physical properties including bulk density, total porosity and unsaturated hydraulic conductivity. The High biosolids treatment also increased concentrations of soil total cadmium (Cd), chromium (Cr), copper (Cu) and lead (Pb) at 25–50 cm depth, but these concentrations were still considered very low for a soil. Ecotoxicological assessment showed no significant adverse effects of biosolids application on either the reproduction of springtails (Folsomia candida) or substrate utilisation ability of the soil microbial community, indicating no negative ecological impact of bisolids-derived heavy metals or triclosan. This study demonstrated that repeated application of biosolids to a plantation forest on a poor sandy soil could significantly improve soil fertility, tree nutrition and pine productivity. However, the long-term fate of biosolids-derived N, P and litter-retained heavy metals needs to be further monitored in the receiving

  4. Ecological impacts of long-term application of biosolids to a radiata pine plantation

    Energy Technology Data Exchange (ETDEWEB)

    Xue, Jianming, E-mail: jianming.xue@scionresearch.com [Scion, Private Bag 29237, Christchurch (New Zealand); Kimberley, Mark O., E-mail: mark.kimberley@scionresearch.com [Scion, Private Bag 3020, Rotorua (New Zealand); Ross, Craig, E-mail: rossc@landcareresearch.co.nz [Landcare, Private Bag 11052, Palmerston North (New Zealand); Gielen, Gerty, E-mail: gerty.gielen@scionresearch.com [Scion, Private Bag 3020, Rotorua (New Zealand); Tremblay, Louis A., E-mail: louis.tremblay@cawthron.org.nz [Cawthron Institute, Private Bag 2, Nelson (New Zealand); School of Biological Sciences, University of Auckland, PO Box 92019, Auckland 1142 (New Zealand); Champeau, Olivier, E-mail: olivier.champeau@cawthron.org.nz [Cawthron Institute, Private Bag 2, Nelson (New Zealand); Horswell, Jacqui, E-mail: jacqui.horswell@esr.cri.nz [ESR, P O Box 50-348, Porirua (New Zealand); Wang, Hailong, E-mail: hailong@zafu.edu.cn [Scion, Private Bag 3020, Rotorua (New Zealand); Key Laboratory of Soil Contamination Bioremediation of Zhejiang Province, Zhejiang Agricultural and Forestry University, Lin' an, Hangzhou, Zhejiang Province 311300 (China)

    2015-10-15

    Assessment of the ecological impact of applying biosolids is important for determining both the risks and benefits. This study investigated the impact on soil physical, chemical and biological properties, tree nutrition and growth of long-term biosolids applications to a radiata pine (Pinus radiata D. Don) plantation growing on a Sandy Raw Soil in New Zealand. Biosolids were applied to the trial site every 3 years from tree age 6 to 19 years at three application rates: 0 (Control), 300 (Standard) and 600 (High) kg nitrogen (N) ha{sup −1}, equivalent to 0, 3 and 6 Mg ha{sup −1} of dry biosolids, respectively. Tree nutrition status and growth have been monitored annually. Soil samples were collected 13 years after the first biosolids application to assess the soil properties and functioning. Both the Standard and High biosolids treatments significantly increased soil (0–50 cm depth) total carbon (C), N, and phosphorus (P), Olsen P and cation exchange capacity (CEC), reduced soil pH, but had no significant effects on soil (0–20 cm depth) physical properties including bulk density, total porosity and unsaturated hydraulic conductivity. The High biosolids treatment also increased concentrations of soil total cadmium (Cd), chromium (Cr), copper (Cu) and lead (Pb) at 25–50 cm depth, but these concentrations were still considered very low for a soil. Ecotoxicological assessment showed no significant adverse effects of biosolids application on either the reproduction of springtails (Folsomia candida) or substrate utilisation ability of the soil microbial community, indicating no negative ecological impact of bisolids-derived heavy metals or triclosan. This study demonstrated that repeated application of biosolids to a plantation forest on a poor sandy soil could significantly improve soil fertility, tree nutrition and pine productivity. However, the long-term fate of biosolids-derived N, P and litter-retained heavy metals needs to be further monitored in the

  5. EFFECTIVENESS OF A SUPERFICIAL TREATMENT USING BIFENTHRIN TO PROTECT RADIATA PINE FRAMING FROM DAMAGE BY SUBTERRANEAN AND DRYWOOD TERMITES IN INDONESIA

    Directory of Open Access Journals (Sweden)

    Paimin Sukartana

    2010-06-01

    Full Text Available Various experimental testing procedures were undertaken in Indonesia to determine the effectiveness of a patented superficial (envelope treatment using bifenthrin to protect radiata pine framing material from damage by two species of subterranean termites (Macrotermes gilvus and Coptotermes curvignathus and one species of drywood termite (Cryptotermes cynocephalus. Lengths of framing material (Pinus radiata sapwood were commercially treated to the targeted retention of 0.02% m/m of bifenthrin in the outer 2 mm depth penetration zone of the material. The treated and untreated materials were subsequently cut into test specimens and exposed to M. gilvus in the field and a semi-laboratory trial, to C. curvignathus in the laboratory and a semi-laboratory trial and to C. cynocephalus in a laboratory trial. No supplementary treatment was performed on the exposed cut ends of the treated test specimens. The results from the trials clearly demonstrated that the superficial treatment of bifenthrin seemed effective in protecting test specimens of radiata pine framing material from significant damage by Indonesia’s most notorious termite species that often causes serious economic loss to the timbers. Termites were unable to damage any of the bifenthrin-treated surfaces of test specimens. Any obser ved damage by termites, albeit minor, was in all cases confined to the exposed cut ends of test specimens. In contrast, attack by termites on the untreated control test specimens caused damage of the samples ranging from light to heavy.

  6. Hylastes ater (Curculionidae: Scolytinae Affecting Pinus radiata Seedling Establishment in New Zealand

    Directory of Open Access Journals (Sweden)

    Stephen D. Reay

    2012-01-01

    Full Text Available The introduced pine bark beetle Hylastes ater has been present in New Zealand for around 100 years. The beetle has been a minor pest on pines. Research was undertaken to control the pest in the 1950s–1970s, with a biological control agent introduced with limited success. Following a reasonably long period with minimal research attention, renewed interest in developing a better understanding of the pest status was initiated in the mid to late 1990s. Subsequently, a significant amount of research was undertaken, with a number of studies exploring the role of this pest of exotic forests in New Zealand. These studies ranged from attempting to quantify damage to seedlings, evaluate the role of the beetle in vectoring sapstain fungi, explore options for management, and evaluate the potential for chemical and biological control. From these studies, a number of findings were made that are relevant to the New Zealand exotic forest industry and shed new light onto the role of secondary bark beetles globally.

  7. MICROINJERTO IN VITRO COMO UNA TECNICA DE REVIGORIZACION DE ARBOLES ELITES DE PINUS RADIATA D. DON.

    OpenAIRE

    MATERAN OVIEDO, MARIA ELENA; MATERAN OVIEDO, MARIA ELENA

    2008-01-01

    Es bien conocido, que los ciclos de vida de muchas especies perennes comprenden dos fases en las que determinadas características morfológicas y fisiológicas son bastante distintas. Después de la germinación, la mayoría de las plántulas anuales y perennes 106p.

  8. Solute accumulation and elastic modulus changes in six radiata pine breeds exposed to drought.

    Science.gov (United States)

    De Diego, N; Sampedro, M C; Barrio, R J; Saiz-Fernández, I; Moncaleán, P; Lacuesta, M

    2013-01-01

    Drought is one of the main abiotic factors that determine forest species growth, survival and productivity. For this reason, knowledge of plant drought response and the identification of physiological traits involved in stress tolerance will be of interest to breeding programs. In this work, several Pinus radiata D. Don breeds from different geographical origins were evaluated along a water stress period (4 weeks) and subsequent rewatering (1 week), showing different responses among them. Leaf water potential (Ψ(leaf)) and osmotic potential decreases were accompanied by a variation in the total relative water content (RWC, %). The most tolerant breeds presented the lowest leaf water potential and RWC at turgor loss point, and showed the lowest elastic modulus (ε) values. A high ε value was a characteristic of a less-drought-tolerant plant and was related to membrane alterations (high electrolyte leakage percentages) that could favor cell water loss. Of the group of solutes that contributed to osmotic adjustment, soluble carbohydrates were the most abundant, although stressed plants also increased their content of free amino acids [mainly proline (Pro) and glutamic acid (Glu), and γ-aminobutyric acid (GABA)] and free polyamines. In addition, the most sensitive breeds had a higher GABA/Glu ratio. After rewatering, Pro and GABA were higher in rehydrated plants than in controls.

  9. Selection and evaluation of Rhizobial strains of Vigna radiata L ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-10-20

    Oct 20, 2008 ... Selection and evaluation of Rhizobial strains of Vigna radiata L. beneficial to ... This study aimed to select suitable strains that can be used as inoculants to enhance legume production and simultaneously reduce the use of ... contributor to natural or biological N2 fixation and allows legumes to grow in the ...

  10. The effects of high energy radiation on the pulping properties of Pinus radiation and Eucalyptus regnans

    International Nuclear Information System (INIS)

    McLaren, K.G.; Garland, C.P.; Higgins, H.G.

    1976-01-01

    Studies have been made of the effects of high energy radiation on the pulping behaviour of Eucalyptus regnans and Pinus radiata. Pre-irradiation of wood chips with small doses of 60 Co gamma radiation (up to about 0.2 Mrad) caused little degradation of the cellulose, and had only minor effects on the kraft pulping properties of both wood species. Pulp yield, Kappa number and strength properties of the pulps showed little change. There was also little effect on the bisulphite cooking of Pinus radiata. As the dose was increased to 1 Mrad, degradation of cellulose (as indicated by degree of polymerisation measurements) became significant, and Kraft pulp yields from both woods showed small reductions. The Kappa number and physical properties of these pulps were little affected at this dose level. A gamma radiation dose of 10 Mrad produced marked depolymerisation of the cellulose, and big reductions in kraft and neutral sulphite semi-chemical pulp yields. The kraft pulps showed a much higher lignin content. Some low dose (0.15 Mrad) irradiations on thin chips were carried out with a 1 MeV electron accelerator. In contrast to comparable gamma irradiations, this treatment produced discernible changes in kraft pulping behaviour. The pulp yield, under the same cooking conditions, appears to be slightly higher, but the Lignin content of the pulp was increased. (Author)

  11. Transcriptional Slippage and RNA Editing Increase the Diversity of Transcripts in Chloroplasts: Insight from Deep Sequencing of Vigna radiata Genome and Transcriptome.

    Directory of Open Access Journals (Sweden)

    Ching-Ping Lin

    Full Text Available We performed deep sequencing of the nuclear and organellar genomes of three mungbean genotypes: Vigna radiata ssp. sublobata TC1966, V. radiata var. radiata NM92 and the recombinant inbred line RIL59 derived from a cross between TC1966 and NM92. Moreover, we performed deep sequencing of the RIL59 transcriptome to investigate transcript variability. The mungbean chloroplast genome has a quadripartite structure including a pair of inverted repeats separated by two single copy regions. A total of 213 simple sequence repeats were identified in the chloroplast genomes of NM92 and RIL59; 78 single nucleotide variants and nine indels were discovered in comparing the chloroplast genomes of TC1966 and NM92. Analysis of the mungbean chloroplast transcriptome revealed mRNAs that were affected by transcriptional slippage and RNA editing. Transcriptional slippage frequency was positively correlated with the length of simple sequence repeats of the mungbean chloroplast genome (R2=0.9911. In total, 41 C-to-U editing sites were found in 23 chloroplast genes and in one intergenic spacer. No editing site that swapped U to C was found. A combination of bioinformatics and experimental methods revealed that the plastid-encoded RNA polymerase-transcribed genes psbF and ndhA are affected by transcriptional slippage in mungbean and in main lineages of land plants, including three dicots (Glycine max, Brassica rapa, and Nicotiana tabacum, two monocots (Oryza sativa and Zea mays, two gymnosperms (Pinus taeda and Ginkgo biloba and one moss (Physcomitrella patens. Transcript analysis of the rps2 gene showed that transcriptional slippage could affect transcripts at single sequence repeat regions with poly-A runs. It showed that transcriptional slippage together with incomplete RNA editing may cause sequence diversity of transcripts in chloroplasts of land plants.

  12. Metabolism of 14C-aspartate during shoot bud formation in cultured cotyledon explants of radiata pine

    International Nuclear Information System (INIS)

    Konschuh, M.N.; Thorpe, T.A.

    1997-01-01

    Aspartate metabolism was investigated in excised cotyledons of radiata pine (Pinus radiate D. Don). These cotyledons were cultured under shoot-forming (plus N 6 -benzyladenine, SF), non-shoot-forming (minus N 6 -benzyladenine, NSF) and unresponsive (plus N 6 -benzyladenine, OLD) conditions, then incubated with [ 14 C]-aspartate for 3-h pulse treatments followed by 3-h chase treatments with cold aspartate. The majority of label was recovered in the CO 2 , amino acid, organic acid and pellet fractions. Uptake was greatest in all tissue types early in culture. Most (over 80%) of the [ 14 C 9-aspartate taken up by the tissues was converted to CO 2 at day 0 in SF and NSF tissues. CO 2 accounted for less than 50% of the total radioactivity in other tissues. Greater incorporation into fractions was observed in SF tissues during promeristemoid formation, while in NSF tissues the greatest incorporation was observed during a period of rapid elongation. Generally, less incorporation was observed in OLD cotyledons than in SF and NSF cotyledons. Analysis of the amino acid fraction showed that labelled aspartate was converted to other amino acids, mainly glutamate, glutamine, asparagine and 4-aminobutyric acid. (au)

  13. Mechanism of Resistance in Mungbean [Vigna radiata (L. R. Wilczek var. radiata] to bruchids, Callosobruchus spp. (Coleoptera: Bruchidae

    Directory of Open Access Journals (Sweden)

    Abdul R. War

    2017-06-01

    Full Text Available Mungbean [Vigna radiata (L. R. Wilczek var. radiata] is an important pulse crop in Asia, and is consumed as dry seeds and as bean sprouts. It is an excellent source of digestible protein. Bruchids [Callosobruchus chinensis (L. and Callosobruchus maculatus (F.] are the important pests of mungbean and cause damage in the field and in storage. Bruchid infestation reduces the nutritional and market value of the grain and renders seeds unfit for human consumption, agricultural and commercial uses. These pests are controlled mainly by fumigation with highly toxic chemicals such as carbon disulfide, phosphene, and methyl bromide, or by dusting with several other insecticides, which leave residues on the grain, thus, threatening food safety. Some plant-based extracts have been found useful in controlling bruchids, but are not fully successful due to their short-term activity, rapid degradability, and potentially negative effect on seed germination. Although some wild sources of bruchid resistance in mungbean have been reported, which have been used to develop bruchid- resistant lines, undesirable genetic linkages threaten the proper exploitation of genetic diversity from wild germplasm into commercial cultivars. Further, biotype variation in bruchids has rendered some mungbean lines susceptible that otherwise would have been resistant to the pest. Host plant resistance is a cost-effective and a safe alternative to control bruchids in mungbean and is associated with morphological, biochemical, and molecular traits. These traits affect insect growth and development, thereby, reduce the yield losses by the pests. Understanding the defense mechanisms against insect pests could be utilized in exploiting these traits in crop breeding. This review discusses different traits in mungbean involved in defense against bruchids and their utility in pest management. We also highlight the breeding constraints for developing bruchid-resistant mungbean and how can these

  14. Forested habitat preferences by Chilean citizens: Implications for biodiversity conservation in Pinus radiata plantations Preferencia por hábitats forestales por ciudadanos chilenos: Implicancias para la conservación de biodiversidad en plantaciones de Pinus radiata

    Directory of Open Access Journals (Sweden)

    NICOLE PÜSCHEL-HOENEISEN

    2012-06-01

    Full Text Available The need for conservation outside protected areas has prompted the modification of productive practices to allow the maintenance of wild biota in productive landscapes such as those associated to timber production. Forest plantations could cooperate in conserving biodiversity outside protected areas if they have a developed understory. However, the success of the production changes depends on the social support they receive. Therefore, we evaluate Chilean citizens' preference for five habitats of different types of forest management. In addition, we assessed perceptions regarding the relationship between pine plantations and native wildlife through surveys administered in Chillán, Santiago and six rural localities in the VII and VIII region. Despite there is not a unanimous opinion regarding pine plantations as a threat to biodiversity, people prefer pine plantations that serve as habitat for endangered fauna. In fact, they agree on paying more for forest products to contribute to conservation in forest plantations, and actually prefer plantations with a developed understory better than those without it. This would suggest that measures aimed at conservation in forest plantations could be supported by the Chilean society.La necesidad de la conservación fuera de áreas protegidas ha llevado a la modificación de las prácticas productivas para permitir el mantenimiento de la biota silvestre en paisajes productivos tales como los asociados a la producción de madera. Las plantaciones forestales podrían cooperar en la conservación de la biodiversidad fuera de áreas protegidas si tienen un sotobosque desarrollado. Sin embargo, el éxito de los cambios en la producción depende del apoyo social que estos reciben. Así, evaluamos la preferencia por cinco paisajes con diferentes tipos de manejo forestal. Además, se evaluó la percepción acerca de la relación entre las plantaciones de pino y la fauna nativa a través de encuestas realizadas en Chillán, Santiago y seis localidades rurales de la VII y VIII Región. Pese a no haber una opinión pública unánime respecto a considerar las plantaciones de pino como una amenaza a la biodiversidad, las personas en general prefieren las plantaciones de pino que sirven de hábitat para la fauna en peligro de extinción. De hecho, están de acuerdo en pagar más por productos forestales que contribuyan a la conservación en plantaciones forestales, prefiriendo especialmente plantaciones forestales con un sotobosque desarrollado. Estos resultados sugieren que las medidas destinadas a la conservación en las plantaciones forestales podrían ser apoyadas por la sociedad chilena.

  15. Endogenous cytokinin and auxin profiles during in vitro organogenesis from vegetative buds of Pinus radiata adult trees

    Czech Academy of Sciences Publication Activity Database

    Montalban, I.A.; Novák, Ondřej; Rolčík, Jakub; Strnad, Miroslav; Moncalean, P.

    2013-01-01

    Roč. 148, č. 2 (2013), s. 214-231 ISSN 0031-9317 Institutional research plan: CEZ:AV0Z50380511 Keywords : ACTINIDIA-DELICIOSA * SHOOT ORGANOGENESIS * AROMATIC CYTOKININS Subject RIV: EF - Botanics Impact factor: 3.262, year: 2013

  16. Serrated leaf mutant in mungbean (Vigna radiata (L) Wilczek)

    International Nuclear Information System (INIS)

    Malik, I.A.; Ghulam, Sarwar; Yousaf, Ali; Saleem, M.

    1988-01-01

    Dry dormant seeds of mungbean (Vigna radiata (L) Wilczek) were treated with gamma rays (15, 30 and 60 kR). The serrated leaf mutation was noticed in M 2 of cultivar Pak 32 treated with 60 kR. Cf 14 plants, 3 showed the altered leaf structure and the others were normal. The feature of this mutant was the deep serration of leaflet margins. The mutant had large thick leaflets with prominent venation. The mutant bred true in the M 3 and successive generation. Details of the morphological characteristics of the mutant are presented. The mutant exhibited slower growth particularly during the early stages of development, flowered later and attained shorter height. There was an increase in the number of pods, in seed weight and in seed protein content, but number of seed per pod was considerably reduced. The seed coat colour showed a change from green to yellowish green. In the mutant's flowers the stamina were placed much below the stigma level and the stigma sometimes protruded the corolla. Outcrossing of 4% recorded in some of the mutant lines revealed a reduced cleistogamy. The low number of seeds per pod in the mutant could be due to reduced pollen fertility. The mutant behaved as monogenic recessive. The symbols SL/sl are proposed for this allelic pair. The mutant may have use as a green manure crop because of its large foliage and for the breeders as a genetic marker

  17. Some physicochemical characteristics of pinus (Pinus halepensis Mill., Pinus pinea L., Pinus pinaster and Pinus canariensis) seeds from North Algeria, their lipid profiles and volatile contents.

    Science.gov (United States)

    Kadri, Nabil; Khettal, Bachra; Aid, Yasmine; Kherfellah, Souraya; Sobhi, Widad; Barragan-Montero, Veronique

    2015-12-01

    Physicochemical characteristics of seeds of some pinus species (Pinus halepensis Mill., Pinus pinea L., Pinus pinaster and Pinus canariensis) grown in North Algeria were determined. The results showed that the seeds consist of 19.8-36.7% oil, 14.25-26.62% protein, 7.8-8.6% moisture. Phosphorus, potassium and magnesium were the predominant elements present in seeds. Pinus seed's oil physicochemical properties show acid values (4.9-68.9), iodine values (93.3-160.4) and saponification values (65.9-117.9). Oil analysis showed that the major unsaturated fatty acids for the four species were linoleic acid (30-59%) and oleic acid (17.4-34.6%), while the main saturated fatty acid was palmitic acid (5-29%). Gas Chromatography and Mass Spectrometry analysis of P. halepensis Mill., P. pinaster and P. canariensis volatile oils indicated that the major volatile compound was the limonene with relative percentage of 3.1, 7.5 and 10.8, respectively. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. A review of Sphaeropsis sapinea occurrence on Pinus species in Bulgaria

    Directory of Open Access Journals (Sweden)

    Margarita Georgieva

    2016-12-01

    Full Text Available Sphaeropsis sapinea (Fr. Dyko & Sutton causes shoot blight and canker disease throughout the world on conifers predisposed by stress. The disease is most important to Pinus species that are affected from the seedling stage in nurseries to mature trees in ornamental planting, forest plantations and natural stands. In Bulgaria, the first findings of the disease were noted in 1989 on Pinus nigra plantations in the North-eastern part of the country. Over the past few years, new emergency and severe damages have developed rapidly as a consequence of the prolonged drought periods during the last growing seasons. The high existence of S. sapinea outbreaks contributed considerably to the physiological weakness of pine trees that become more susceptible to attack by aggressive xylophages and other fungal pathogens. Disease occurrence and its pathogenicity are economically important affecting a number of pine trees from all ages. In Bulgaria, S. sapinea has been obtained on six pine species P. nigra, P. strobus, P. radiata, P. ponderosa, P. pinaster and P. halepensis. The aim of this review is to present the available knowledge on distribution, host specificity, biology, ecology, management of the disease, and to discuss its current prevalence and pathogenicity effect on pine species in Bulgaria.

  19. PpRT1: the first complete gypsy-like retrotransposon isolated in Pinus pinaster.

    Science.gov (United States)

    Rocheta, Margarida; Cordeiro, Jorge; Oliveira, M; Miguel, Célia

    2007-02-01

    We have isolated and characterized a complete retrotransposon sequence, named PpRT1, from the genome of Pinus pinaster. PpRT1 is 5,966 bp long and is closely related to IFG7 gypsy retrotransposon from Pinus radiata. The long terminal repeats (LTRs) have 333 bp each and show a 5.4% sequence divergence between them. In addition to the characteristic polypurine tract (PPT) and the primer binding site (PBS), PpRT1 carries internal regions with homology to retroviral genes gag and pol. The pol region contains sequence motifs related to the enzymes protease, reverse transcriptase, RNAseH and integrase in the same typical order known for Ty3/gypsy-like retrotransposons. PpRT1 was extended from an EST database sequence indicating that its transcription is occurring in pine tissues. Southern blot analyses indicate however, that PpRT1 is present in a unique or a low number of copies in the P. pinaster genome. The differences in nucleotide sequence found between PpRT1 and IFG7 may explain the strikingly different copy number in the two pine species genome. Based on the homologies observed when comparing LTR region among different gypsy elements we propose that the highly conserved LTR regions may be useful to amplify other retrotransposon sequences of the same or close retrotransposon family.

  20. Vigna radiata as a New Source for Biotransformation of Hydroquinone to Arbutin

    Directory of Open Access Journals (Sweden)

    Zahra Tofighi, Mohsen Amini, Mahzad Shirzadi, Hamideh Mirhabibi, Negar Ghazi Saeedi, Narguess Yassa

    2016-06-01

    Full Text Available Background: The suspension culture of Vigna radiata was selected for biotransformation of hydroquinone to its β-D-glucoside form (arbutin as an important therapeutic and cosmetic compound. Methods: The biotransformation efficiency of a Vigna radiata cell culture in addition to different concentrations of hydroquinone (6-20 mg/100 ml was investigated after 24 hours in comparison to an Echinacea purpurea cell culture and attempts were made to increase the efficacy of the process by adding elicitors. Results: Arbutin was accumulated in cells and found in the media only in insignificant amounts. The arbutin content of the biomass extracts of V. radiata and E. purpurea was different, ranging from 0.78 to 1.89% and 2.00 to 3.55% of dry weight, respectively. V. radiata demonstrated a bioconversion efficiency of 55.82% after adding 8 mg/100 ml precursor, which was comparable with result of 69.53% for E. purpurea cells after adding 10 mg/100 ml hydroquinone (P>0.05. In both cultures, adding hydroquinone in two portions with a 24-hour interval increased the biotransformation efficiency. Different concentrations of methyl jasmonate (25, 50, and 100 µM and chitosan (50 and 100 µg/ml as elicitors increased the bio-efficiency percentage of the V. radiata culture in comparison with the flask containing only hydroquinone. Conclusion: This is the first report of the biotransformation possibility of V. radiata cultures. It was observed the bioconversion capacity increased by adding hydroquinone in two portions, which was comparable to adding an elicitor.

  1. Primer Registro de Tamarixia radiata (Waterston, 1922 (Hymenoptera: Eulophidae en Colombia Record of Tamarixia radiata (Waterston, 1922 (Hymenoptera: Eulophidae in Colombia

    Directory of Open Access Journals (Sweden)

    Everth Emilio Ebratt Ravelo

    2011-12-01

    Full Text Available Tamarixia radiata es un ectoparasitoide idiobionte de Diaphorina citri Kuwayama (Hemiptera: Psyllidae; la especie fue descrita a partir de material recogido en la India. Ha sido introducida en muchas regiones intencional o accidentalmente para el control biológico de D. citri, incluyendo algunos países asiáticos. Se sabe de su presencia en el sur de África y las Américas. En Suramérica, se reportó en Brasil, Argentina y ahora en Colombia.Tamarixia radiata is an ectoparasitoid idiobionte of Diaphorina citri Kuwayama (Hemiptera: Psyllidae; the species was described from material collected in India. It has been introduced in many regions intentionally or accidentally, to biological control of D. citri, including some Asian countries. It is know about its presence in southern Africa and the Americas. In South America, it is present in Brazil, Argentina and now in Colombia.

  2. Population structure and historical demography of the thorny skate (Amblyraja radiata, Rajidae) in the North Atlantic

    NARCIS (Netherlands)

    Chevolot, M.; Wolfs, P.H.J.; Palsson, J.; Rijnsdorp, A.D.; Stam, W.T.; Olsen, J.L.

    2007-01-01

    Population genetic structure of the thorny skate (Amblyraja radiata) was surveyed in >300 individuals sampled from Newfoundland, Iceland, Norway, the Kattegat and the central North Sea. A 290-bp fragment of the mt cytochrome-b gene was first screened by SSCP. Sequences of SSCP haplotypes revealed

  3. Population structure and historical demography of the thorny skate (Amblyraja radiata, Rajidae) in the North Atlantic

    NARCIS (Netherlands)

    Chevolot, Malia; Wolfs, Peter H. J.; Palsson, Jonbjorn; Rijnsdorp, Adriaan D.; Stam, Wytze T.; Olsen, Jeanine L.; Palson, J.

    Population genetic structure of the thorny skate (Amblyraja radiata) was surveyed in > 300 individuals sampled from Newfoundland, Iceland, Norway, the Kattegat and the central North Sea. A 290-bp fragment of the mt cytochrome-b gene was first screened by SSCP. Sequences of SSCP haplotypes revealed

  4. Potential biochemical markers for selection of disease resistance in Vigna radiata

    International Nuclear Information System (INIS)

    Badere, R.S.; Koche, D.K.; Choudhary, A.D.; Pawar, S.E.

    2001-01-01

    The Vigna radiata (L.) Wilczek (Green gram), a major pulse crop is prone to damaging diseases caused by Erysiphe polygoni, Cercospora canescens and Rhizoctonia sp. Therefore, the development of multiple resistance is a major breeding objective in green gram. Resistance to powdery mildew has already been developed, however, there are no reports on the development of resistance to Cercospora in green gram. Owing to limitation of conventional screening methods, the improvement for multiple disease resistance is inadequate, in this crop. It needs an efficient and quick selection method, for screening the plant population at an early stage. It is well established that the resistant interaction, in plants, involves accumulation of antibiotic compound phytoalexin (Genestein in Vigna radiata) and induction of enzymes such as β-1,3 gulcanase and Chitinases. These compounds are not only induced by pathogens but also pathogen-derived elicitors. These biochemical compounds can be used as resistance indicative biochemical markers for screening the natural or mutagen induced genetic diversity in populations of Vigna radiata in non-destructive manner. It, however, needs a systematic study of plant defense response. This paper deals with the response of resistant and susceptible cultivars of vigna radiata to Cercospora elicitor and development of non-destructive selection method for disease resistance. (author)

  5. Reconocimiento de Gestos usando Cámaras de Profundidad

    OpenAIRE

    JHONSON GARCÍA, FIORELLA ANNETH

    2017-01-01

    El proyecto pretende detectar un repertorio de gestos a partir de nubes de puntos 3D obtenidas con cámaras de profundidad tipo Kinect. Jhonson García, FA. (2017). Reconocimiento de Gestos usando Cámaras de Profundidad. http://hdl.handle.net/10251/91700 TFGM

  6. Discovering and verifying DNA polymorphisms in a mung bean [V. radiata (L. R. Wilczek] collection by EcoTILLING and sequencing

    Directory of Open Access Journals (Sweden)

    Dean Rob E

    2008-06-01

    Full Text Available Abstract Background Vigna radiata, which is classified in the family Fabaceae, is an important economic crop and a dietary staple in many developing countries. The species radiata can be further subdivided into varieties of which the variety sublobata is currently acknowledged as the putative progenitor of radiata. EcoTILLING was employed to identify single nucleotide polymorphisms (SNPs and small insertions/deletions (INDELS in a collection of Vigna radiata accessions. Findings A total of 157 DNA polymorphisms in the collection were produced from ten primer sets when using V. radiata var. sublobata as the reference. The majority of polymorphisms detected were found in putative introns. The banding patterns varied from simple to complex as the number of DNA polymorphisms between two pooled samples increased. Numerous SNPs and INDELS ranging from 4–24 and 1–6, respectively, were detected in all fragments when pooling V. radiata var. sublobata with V. radiata var. radiata. On the other hand, when accessions of V. radiata var. radiata were mixed together and digested with CEL I relatively few SNPs and no INDELS were detected. Conclusion EcoTILLING was utilized to identify polymorphisms in a collection of mung bean, which previously showed limited molecular genetic diversity and limited morphological diversity in the flowers and pod descriptors. Overall, EcoTILLING proved to be a powerful genetic analysis tool providing the rapid identification of naturally occurring variation.

  7. Reconocimiento de Huellas Dactilares Usando Características Locales

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    Gualberto Aguilar

    2008-01-01

    Full Text Available El reconocimiento de huellas dactilares es uno de los métodos más populares usados con mayor grado de éxito para la identificación de personas. La huella dactilar tiene características únicas llamadas minucias, las cuales son puntos donde los bordes terminan o se dividen. Los sistemas de identificación que usan patrones biométricos de huella dactilar se denominan AFIS (Sistema de Identificación Automático de Huella Dactilar. En este trabajo se realizó un sistema para reconocimiento de huella dactilar usando combinación de Transformada Rápida de Fourier (FFT con Filtros de Gabor para aclarar la imagen y después un novedoso método para el reconocimiento usando características locales.

  8. Diaphorina citri (Kuwayama, 1907 and Tamarixia radiata (Waterson, 1922 in citrus crops of Cundinamarca, Colombia

    Directory of Open Access Journals (Sweden)

    Ebratt Ravelo Everth Emilio

    2011-12-01

    Full Text Available

    The presence of Diaphorina citri Kuwayama (Hemiptera: Psyllidae and Tamarixia radiata (Waterston (Hymenoptera: Eulophidae was recorded in citrus crops of Cundinamarca, Colombia. This work is to know the geographic distribution of D. citri and an initial record of the parasitoid T. radiata in citrus producing areas of this department.

  9. Bioecology of the fungus Sphaeropsis sapinea dyko & Sutton - agents of pinus species decline

    Directory of Open Access Journals (Sweden)

    Milijašević Tanja

    2002-01-01

    Full Text Available Sphaeropsis sapinea is a cosmopolitan fungus, identified in more than 50 countries of the world, on all continents, but it is primarily the species of warm lands. It is also a polyphagous fungus recorded from 11 coniferous genera. The most endangered and the most frequent host plants are Pinus species - it occurs on 48 pine species, among which the most susceptible are Pinus Radiata, P. nigra, P. sylvestris, P. ponderosa, P. resinosa, P. mugo, P. pinaster and P. elliotti. The greatest damage is caused on the introduced Pinus species and on those cultivated in artificial plantations, shelterbelts and in urban environments. In Yugoslavia S. sapinea is widely distributed both in the continental and in the Mediterranean parts. It was identified from ten pine species and six hosts from other coniferous genera. By the study in our country, the new hosts of this fungus were detected - Pinus jeffrey, P. peuce and P. heldreichii. The most endangered species in our country is Austrian pine, both in urban environments, and in plantations The symptoms of the disease are bud wilt, curling, stunting and necrosis of current year shoots and needles, dieback of top shoots, parts of crown or tree tops, branch and stem bark canker, root collar rot on the young plants in nurseries and their dying. This fungus also prevents seed germination of Pinus species and causes blue sap stain of the freshly cut wood, although sap stain was also observed on standing trees. More rarely it causes root rot and crown wilt of Pinus species. The main symptoms of infection, both of young plants and older trees, are the dieback of current year shoots S. sapinea can penetrate through buds, bark of young shoots and needles. The critical time of infection is the period from mid April to mid May. Then infection mainly penetrates through the bark of young shoots, which results in their dying. Infection through the needles occurs mainly at the time of their sudden growth or during summer

  10. Thermal hygrometric requirements for the rearing and release of Tamarixia radiata (Waterston (Hymenoptera, Eulophidae

    Directory of Open Access Journals (Sweden)

    Mariuxi Lorena Gómez-Torres

    2014-09-01

    Full Text Available Thermal hygrometric requirements for the rearing and release of Tamarixia radiata (Waterston (Hymenoptera, Eulophidae. Tamarixia radiata is the main agent for the biological control of Diaphorina citri in Brazil with a parasitism rate ranging from 20 to 80%. This study investigated the influence of temperature on the development, fecundity and longevity of adults of T. radiata and the effect of relative humidity (RH on their parasitism capacity and survival rate in the pre-imaginal period. The effect of temperature was assessed in the range between 15 and 35 ± 1ºC, 70 ± 10% RH, and a 14-h photophase. The RH effect was evaluated in the range from 30 to 90 ± 10%, temperature at 25 ± 1ºC, and photophase of 14-h. At 25ºC, circa 166.7 nymphs were parasitized, the highest parasitism capacity observed compared to other treatments. The longest longevity of females was observed at 25ºC, although the rate did not differ in the 20-30ºC temperature range. The threshold temperature (TT was 7.2ºC, and 188.7 degrees-day were required for the development (egg-to-adult period. The parasitism rate and longevity were higher at 50 and 70% of RH. This shows that temperature and RH may affect the parasitism capacity of T. radiata on nymphs of D. citri, which can explain the great parasitism variation for D. citri observed in citrus groves in São Paulo State, Brazil.

  11. Ethanol production from Lycoris radiata Herbert (Amarylllidaceae) residues as a new resource

    International Nuclear Information System (INIS)

    Liu, Shuangping; Ding, Zhongyang; Zhang, Liang; Gu, Zhenghua; Wang, Xiaolan; Sun, Xiaojun; Shi, Guiyang

    2012-01-01

    The large quantities of Lycoris radiata Herbert (Amarylllidaceae) residues, left after the extraction of alkaloids from the bulbs, could threaten the environment if not properly disposed. Therefore, the aim of this research is to investigate the feasibility of bioconversion of L. radiata Herbert residues to ethanol through batch fermentation. In L. radiata Herbert residues, the average contents (g kg −1 ) of non-structural carbohydrates, crude fiber, crude protein, ash, and lignin are 485.1, 177.3, 124.7, 108.9, and 91.0, respectively. Five commercial enzymes (β-glucanase, pectinase, xylanase, phytase and cellulase) were employed in pretreatment process and ethanol bioconversion was evaluated with three yeasts (Saccharomyces cerevisiae, osmotolerant S. cerevisiae and genetically engineered S. cerevisiae constructed to use cellobiose). The results showed that pretreament by β-glucanase successfully facilitated the penetration of α-amylase into ground material. After residues pretreatment by 10 g kg −1 of β-glucanase for 14 h at 50 °C, the viscosity decreased from 1135 to 59 Pa·s which was equivalent to that obtained by the combined whole five enzymes. Further experiments proved that osmotolerant S. cerevisiae was desirable for ethanol production from Lycoris radiate Herbert residues. The results are helpful to develop non-grain bioethanol production. -- Highlights: ► The residuals from Lycoris radiata Herbert bulbs were able to generate 55% ethanol concentrations by yeast fermentation. ► Treatment with xylanase significantly reduced the viscosity more than 10-fold and improved fermentable sugars by ∼35%. ► An osmotolerant Saccharomyces cerevisiae strain that showed improved fermentation rates was identified.

  12. Clinicoradiological study on 59 patients with pure motor hemiparesis due to corona radiata infarcts

    International Nuclear Information System (INIS)

    Nagaoka, Tetsuro; Kawabe, Kiyokazu; Ito, Hirono; Ikeda, Ken

    2009-01-01

    To evaluate the clinicoradiological findings of patients with small infarction at the level of the corona radiata, such patients were divided into three groups: Group A (facio-lingual weakness), Group B (arm-dominant weakness) and Group C (leg-dominant weakness). The anteroposterior position and maximum diameter of the radiata infarcts were assessed by axial T2-weighted imaging. In total, 59 consecutive patients (42 men and 17 women) were selected. The frequency of radiata infarct is 9.4% among cerebral infarct patients. The male/female ratio was 1.3. The mean age (SD) was 68.9 (9.5) years. The number of patients was 23 in Group A, 19 in Group B and 17 in Group C. Group B patients needed aid in their daily life, when compared to Group A and Group C. The cerebrovascular risk profiles demonstrated hypertension in 43 patients (72.9%), diabetes mellitus in 15 (25.4%), current smoking in 22 (37.3%), dyslipidemia in 16 (27.1%) and arterial fibrillation in 11 (18.6%). The clinical subtypes revealed 48 patients with lacunar infarct and 11 with cardiogenic emboli. The somatotopical distribution of motor fibers of Groups A to C was arranged in antero-posterior order. There were 24 patients with right lesions and 35 with left lesions. The size of the left infarcts was significantly smaller than that of the right infarcts. The clinical outcome of patients with arm-dominant weakness was relatively poor as compared to that of patients with dysarthria or leg-dominant weakness. The neuroradiological data suggest that left radiata infarct is smaller and more frequent than right infarct. (author)

  13. Clinicoradiological study on 59 patients with pure motor hemiparesis due to corona radiata infarcts

    Energy Technology Data Exchange (ETDEWEB)

    Nagaoka, Tetsuro; Kawabe, Kiyokazu; Ito, Hirono; Ikeda, Ken [Toho Univ., Omori Medical Center, Tokyo (Japan)

    2009-07-15

    To evaluate the clinicoradiological findings of patients with small infarction at the level of the corona radiata, such patients were divided into three groups: Group A (facio-lingual weakness), Group B (arm-dominant weakness) and Group C (leg-dominant weakness). The anteroposterior position and maximum diameter of the radiata infarcts were assessed by axial T2-weighted imaging. In total, 59 consecutive patients (42 men and 17 women) were selected. The frequency of radiata infarct is 9.4% among cerebral infarct patients. The male/female ratio was 1.3. The mean age (SD) was 68.9 (9.5) years. The number of patients was 23 in Group A, 19 in Group B and 17 in Group C. Group B patients needed aid in their daily life, when compared to Group A and Group C. The cerebrovascular risk profiles demonstrated hypertension in 43 patients (72.9%), diabetes mellitus in 15 (25.4%), current smoking in 22 (37.3%), dyslipidemia in 16 (27.1%) and arterial fibrillation in 11 (18.6%). The clinical subtypes revealed 48 patients with lacunar infarct and 11 with cardiogenic emboli. The somatotopical distribution of motor fibers of Groups A to C was arranged in antero-posterior order. There were 24 patients with right lesions and 35 with left lesions. The size of the left infarcts was significantly smaller than that of the right infarcts. The clinical outcome of patients with arm-dominant weakness was relatively poor as compared to that of patients with dysarthria or leg-dominant weakness. The neuroradiological data suggest that left radiata infarct is smaller and more frequent than right infarct. (author)

  14. PHYSIOLOGICAL RESPONSES OF ECKLONIA RADIATA (LAMINARIALES) TO A LATITUDINAL GRADIENT IN OCEAN TEMPERATURE

    DEFF Research Database (Denmark)

    Stæhr, Peter Anton; Wernberg, Thomas

    2009-01-01

    We tested the ability of sporophytes of a small kelp, Ecklonia radiata (C. Agardh) J. Agardh, to adjust their photosynthesis, respiration, and cellular processes to increasingly warm ocean climates along a latitudinal gradient in ocean temperature (~4°C). Tissue concentrations of pigment and nutr......We tested the ability of sporophytes of a small kelp, Ecklonia radiata (C. Agardh) J. Agardh, to adjust their photosynthesis, respiration, and cellular processes to increasingly warm ocean climates along a latitudinal gradient in ocean temperature (~4°C). Tissue concentrations of pigment...... and nutrients decreased with increasing ocean temperature. Concurrently, a number of gradual changes in the metabolic balance of E. radiata took place along the latitudinal gradient. Warm-acclimatized kelps had 50% lower photosynthetic rates and 90% lower respiration rates at the optimum temperature than did...... cool-acclimatized kelps. A reduction in temperature sensitivity was also observed as a reduction in Q10-values from cool- to warm-acclimatized kelps for gross photosynthesis (Q10: 3.35 to 1.45) and respiration (Q10: 3.82 to 1.65). Respiration rates were more sensitive to increasing experimental...

  15. Enhancing Growth of Vigna radiata in the Presence of Pseudomonas aeruginosa Biopolymer and Metarhizium anisopliae Spores

    Directory of Open Access Journals (Sweden)

    Bhagwan N. Rekadwad

    2016-01-01

    Full Text Available Exopolysaccharide producing Pseudomonas aeruginosa NCIM 2945 (PANCL belonging to gamma-proteobacterium and entomopathogenic fungus Metarhizium anisopliae MCC 1129 (MAMCC belonging to Ascomycota were studied for their morphological features biochemical characteristics and plant growth promotion ability. Optimum growth of PANCL was recorded after 24 h at temperature 30°C and pH 7.0. Gram-negative PANCL appeared as white in color, one mm size, circular, opaque, and nonconsistent elevated colonies with entire margin. It has utilized dextrose, fructose, maltose, and sorbitol as carbon source and produced acid in the medium. PANCL was sensitive to Polymyxin B (300 µgm/disc followed by Neomycin (30 µgm/disc, Gentamycin (10 µgm/disc, and Chloramphenicol (30 µgm/disc. PANCL has secreted extracellular lipase, amylase, protease, and exopolysaccharides (EPS. Another fungal strain MAMCC sporulated after 168 h at temperature 30°C and pH 7.0. MAMCC has septate-white mycelium and bears dirty green colored spores. Growth of MAMCC was enhanced in the presence of Neem and Karela-Amla oil (0.1 mL each. Extracellular polysaccharide produced by PANCL and spores of MAMCC promoted growth of dicotyledon Vigna radiata (Mung individually as well as in consortium. Considerable increase in dry weight of Vigna radiata was recorded. Thus, reported PANCL and MAMCC strains have promoted growth Vigna radiata and may be a solution for sustainable agriculture.

  16. Implementación del sistema para municipios, usando pmi

    OpenAIRE

    Cruz Ruiz, Victor Hugo; Buenaño Vera, Sulay Pamela; Freire Cobo, Lenin

    2014-01-01

    El objetivo de informe de graduación, es plantear y describir la implementación de un sistema informático en una entidad municipal usando PMI, siguiendo las pautas definidas en la Guía del PMBOK. Nuestro estudio es aplicar la Metodología Administración de Proyectos PMI a un proyecto ya terminado. Para este proyecto hemos tomado la información de la implementación del Sistema CABILDO ERP en el Gobierno Municipal de San Jacinto de Yaguachi, realizado por la empresa ecuatoriana...

  17. Programação funcional usando Java

    OpenAIRE

    Jorge Juan Zavaleta Gavidia

    1997-01-01

    Desde a introdução da World Wide Web para o mundo nos inícios de 1990, usando a Internet como uma rede para transferir dados, empregando uma forma de expressão chamada de Hipertexto, a qual liga as informações relacionadas e combinadas com multimídia, os Webs resultantes têm aberto novas possibilidades de expressão e comunicação. A quantidade de tráfego de dados na Web e o número de computadores ofertando informação vem crescendo dramaticamente, mas falta expressividade e qualidade interativa...

  18. Volcanic mercury in Pinus canariensis

    Science.gov (United States)

    Rodríguez Martín, José Antonio; Nanos, Nikos; Miranda, José Carlos; Carbonell, Gregoria; Gil, Luis

    2013-08-01

    Mercury (Hg) is a toxic element that is emitted to the atmosphere by both human activities and natural processes. Volcanic emissions are considered a natural source of mercury in the environment. In some cases, tree ring records taken close to volcanoes and their relation to volcanic activity over time are contradictory. In 1949, the Hoyo Negro volcano (La Palma-Canary Islands) produced significant pyroclastic flows that damaged the nearby stand of Pinus canariensis. Recently, 60 years after the eruption, we assessed mercury concentrations in the stem of a pine which survived volcano formation, located at a distance of 50 m from the crater. We show that Hg content in a wound caused by pyroclastic impacts (22.3 μg kg-1) is an order of magnitude higher than the Hg concentrations measured in the xylem before and after the eruption (2.3 μg kg-1). Thus, mercury emissions originating from the eruption remained only as a mark—in pyroclastic wounds—and can be considered a sporadic and very high mercury input that did not affect the overall Hg input in the xylem. In addition, mercury contents recorded in the phloem (9.5 μg kg-1) and bark (6.0 μg kg-1) suggest that mercury shifts towards non-living tissues of the pine, an aspect that can be related to detoxification in volcanism-adapted species.

  19. Volcanic mercury in Pinus canariensis.

    Science.gov (United States)

    Rodríguez Martín, José Antonio; Nanos, Nikos; Miranda, José Carlos; Carbonell, Gregoria; Gil, Luis

    2013-08-01

    Mercury (Hg) is a toxic element that is emitted to the atmosphere by both human activities and natural processes. Volcanic emissions are considered a natural source of mercury in the environment. In some cases, tree ring records taken close to volcanoes and their relation to volcanic activity over time are contradictory. In 1949, the Hoyo Negro volcano (La Palma-Canary Islands) produced significant pyroclastic flows that damaged the nearby stand of Pinus canariensis. Recently, 60 years after the eruption, we assessed mercury concentrations in the stem of a pine which survived volcano formation, located at a distance of 50 m from the crater. We show that Hg content in a wound caused by pyroclastic impacts (22.3 μg kg(-1)) is an order of magnitude higher than the Hg concentrations measured in the xylem before and after the eruption (2.3 μg kg(-1)). Thus, mercury emissions originating from the eruption remained only as a mark-in pyroclastic wounds-and can be considered a sporadic and very high mercury input that did not affect the overall Hg input in the xylem. In addition, mercury contents recorded in the phloem (9.5 μg kg(-1)) and bark (6.0 μg kg(-1)) suggest that mercury shifts towards non-living tissues of the pine, an aspect that can be related to detoxification in volcanism-adapted species.

  20. THE ALLELOPATHIC EFFECT OF SHREDDED LEAVES OF ARTOCARPUS HETEROPHYLLUS AND A. ALTILIS ON VIGNA RADIATA L

    OpenAIRE

    Romilly Margaret Mendez* & Glaxy Ezekel.V

    2017-01-01

    In the present study, an attempt has been made to study the effect of Artocarpus heterophyllus Lam. and Artocarpus altilis (Parkinson) Fosberg on seed germination, seedling growth and total phenolic content of Vigna radiata L. The objective of this study is to assess the rate of germination, growth of the seedlings and the chlorophyll content of the cultivar seeds exposed to four concentrations (10 ppm, 1 ppm, 0.1 ppm and 0.01 ppm) of the leaf extracts of A. heterophyllus and A. altilis. I...

  1. Characterization of seasonal reproductive and stress steroid hormones in wild Radiated Tortoises, Astrochelys radiata.

    Science.gov (United States)

    Currylow, Andrea F T; Rafeliarisoa, Tsilavo H; Louis, Edward E; Stanford, Craig B; Randrianjafizanaka, Soary T; Chinn, Sarah M; Crocker, Daniel E

    2017-11-01

    The critically endangered Radiated Tortoise (Astrochelys radiata) is endemic to the southern coastlines of Madagascar. Once common, wild populations of this tortoise have undergone dramatic declines in recent years. Although there have been studies documenting reproductive activities, reproductive physiological parameters are unknown yet may be crucial in the recovery of the species. Over four research seasons in remote field locations native to A. radiata, we surveyed for, radio-tracked, and sampled wild, free ranging tortoises. We sampled and measured stress and reproductive parameters (corticosterone [CORT], testosterone [T], estradiol-17β [E2], and progesterone [P]) in 311 plasma samples from 203 wild A. radiata, capturing their active period. Generally, hormone concentrations were associated with body condition, temperature, and humidity. There was wide variation in CORT that varied monthly and by group. Juvenile tortoises maintained more than twice the mean basal CORT concentrations than either adult sex, with the most dramatic distinctions in the middle of the wet season. For adult sex hormones, the last months of the dry season and into the wet season when ground humidities are low and just begin to rise prior to temperature declines, male T concentrations gradually increased to a peak before returning to near undetectable values into the dry season. We had limited data for T concentrations in females, but found average T concentrations were much lower than in males and positively correlated with larger female home range sizes. For female hormone cycles, E2 also peaked in the early 1/3 of the wet season along with male T, and was followed by an uptick in P which correlates to the putative ovulatory cycle. Females tracked over four years showed variation in patterns of P, indicating that number and frequency of clutches vary. Our results suggest that 1) there is high species plasticity in response to stress; 2) A. radiata reproductive cycling is somewhat

  2. Induction of chlorophyll chimeras and chlorophyll mutations in mungbean (Vigna radiata) cv. T44

    International Nuclear Information System (INIS)

    Singh, V.P.; Yadav, R.D.S.

    1993-01-01

    Uniform and healthy seeds of mungbean (Vigna radiata) cv. T44 were exposed to varying doses of gamma rays, ethyl methane sulphonate (EMS) and combination treatment of gamma rays with EMS. The data were recorded for seed germination, plant survival, frequency and spectrum of chlorophyll chimeras in M 1 and chlorophyll mutations in M 2 generation. Among all, the combination treatments were found most effective for inducing chlorophyll chimeras and chlorophyll mutations than the gamma rays or EMS alone. Of the mutants under reference, the albino, xantha and chlorina showed monogenic recessive while viridis exhibited digenic recessive inheritance. (author). 8 refs., 2 tabs

  3. Effect of chronic administration of Tamoxifen on fertility in male bonnet monkeys (Macaca radiata).

    Science.gov (United States)

    Rao, A J; Ramachandra, S G; Ramesh, V; Krishnamurthy, H N; Jayaraman, S; Gopalakrishnan, K; Juneja, H S

    1998-01-01

    Administration of Tamoxifen via the Alzet pump at a rate of 50 micrograms hr-1 for 90 days in the adult male bonnet monkeys Macaca radiata had no effect on the serum testosterone concentration determined at 10 AM and 10 PM as well as total sperm count determined at 15-day intervals over a period of 260 days. However, a significant reduction in sperm motility was observed beyond 90 days up until the 225th day. Breeding studies conducted from day 90 to 260 revealed that these males were infertile.

  4. Regeneration of Pinus cubensis Griseb. plants

    Directory of Open Access Journals (Sweden)

    Raima Cantillo Ardebol

    2011-01-01

    Full Text Available Micropropagation of plants from the genus Pinus has been done in several species. However, micropropagation of Pinus cubensis Griseb has not been reported. This species has a great economical importance. Then, the aim of the current research was to achieve in vitro propagation of Pinus cubensis Griseb. to increase the number of individuals in their natural habitat. The concentration of sodium hypochlorite and immersion time were determined for seeds disinfection and embryos establishment. The effect of the presence or absence of the seed coat was also studied. Two growth regulators and three concentrations of each one were tested to achieve the emission of axillary buds in the multiplication phase. Five subcultures every 21 days were done. Rooting and acclimatization were carried out simultaneously. Shoots were individualized and immersed in a rooting solution. Zygotic embryos of P. cubensis wer e dev el o ped in vitro. The highest percentages of disinfection and germination were obtained by introducing the seeds in a solution of sodium hypochlorite at 20% for 15 minutes, planting them after that without the seed coat. The number and length of axillary buds increased by using 22.5 µM of 6-benzylaminopurine and 5.4 µM naphthaleneacetic acid in the multiplication phase. The in vitro propagation of Pinus cubensis Griseb. from zygotic embryos was achieved for the first time. A protocol was also established, reaching 50% of survival in the acclimatization phase. Key words: acclimatization, axillary buds, forestry, multiplication, pinus

  5. Pinus halepensis, Pinus pinaster, Pinus pinea and Pinus sylvestris Essential Oils Chemotypes and Monoterpene Hydrocarbon Enantiomers, before and after Inoculation with the Pinewood Nematode Bursaphelenchus xylophilus.

    Science.gov (United States)

    Rodrigues, Ana M; Mendes, Marta D; Lima, Ana S; Barbosa, Pedro M; Ascensão, Lia; Barroso, José G; Pedro, Luis G; Mota, Manuel M; Figueiredo, A Cristina

    2017-01-01

    Pinewood nematode (PWN), Bursaphelenchus xylophilus, is the causal agent of pine wilt disease, a serious threat to global forest populations of conifers, especially Pinus spp. A time-course study of the essential oils (EOs) of 2-year-old Pinus halepensis, Pinus pinaster, Pinus pinea and Pinus sylvestris following inoculation with the PWN was performed. The constitutive and nematode inoculation induced EOs components were analyzed at both the wounding or inoculation areas and at the whole plant level. The enantiomeric ratio of optically active main EOs components was also evaluated. External symptoms of infection were observed only in P. pinaster and P. sylvestris 21 and 15 days after inoculation, respectively. The EO composition analysis of uninoculated and unwounded plants revealed the occurrence of chemotypes for P. pinaster, P. halepensis and P. sylvestris, whereas P. pinea showed a homogenous EO composition. When whole plants were evaluated for EO and monoterpene hydrocarbon enantiomeric chemical composition, no relevant qualitative and quantitative differences were found. Instead, EO analysis of inoculated and uninoculated wounded areas revealed an increase of sesquiterpenes and diterpenic compounds, especially in P. pinea and P. halepensis, comparatively to healthy whole plants EOs. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  6. Pinus taeda AND Pinus oocarpa PLYWOOD MANUFACTURING WITH FENOL-FORMALDHEYDE RESIN

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2002-01-01

    Full Text Available The objective of this study was to evaluate the quality of the plywood, manufactured from Pinus taeda and Pinus oocarpa, with 20 and 24 years old respectively, using three differentformulations of the fenol-formaldheyde resin. The results of the glue line shearing tests not showed statistical differences between the species and three resin formulations. In the hot water test, all of the boards were classified as “BR” and, the boards of the Pinus oocarpa produced with formulations (1and (3, were classified as “WBP”. The different resin formulations not influenced on the modulus ofelasticity (MOE and modulus of rupture (MOR The boards of Pinus taeda with formulations (1 and(2 showed higher values of MOE in comparison of the Pinus oocarpa. The MOR of the Pinus oocarpa withformulation (2 was higher than Pinus taeda. The mechanical properties of the plywood wereprobably influenced by wood variability related to sapwood and hartwood, thickness of growth ringsand, springwood and summerwood.

  7. Evaluation of the present genetic conservation efforts in Pinus sylvestris, Picea abies, Quercus spp., Fagus sylvatica, and Pinus pinaster

    NARCIS (Netherlands)

    Kramer, K.

    2015-01-01

    Information on genetic diversity and gene conservation activities were combined with climatic data to evaluate the present genetic conservation efforts in Pinus sylvestris, Picea abies, Quercus spp., Fagus sylvatica, and Pinus pinaster. Combinations of climatic variables explained much of the

  8. 28-Homobrassinolide mitigates boron induced toxicity through enhanced antioxidant system in Vigna radiata plants.

    Science.gov (United States)

    Yusuf, Mohammad; Fariduddin, Qazi; Ahmad, Aqil

    2011-11-01

    The objective of this study was to establish relationship between boron induced oxidative stress and antioxidant system in Vigna radiata plants and also to investigate whether brassinosteroids will enhance the level of antioxidant system that could confer tolerance to the plants from the boron induced oxidative stress. The mung bean (V. radiata cv. T-44) plants were administered with 0.50, 1.0 and 2.0 mM boron at 6 d stage for 7 d along with nutrient solution. At 13 d stage, the seedlings were sprayed with deionized water (control) or 10(-8) M of 28-homobrassinolide and plants were harvested at 21 d stage to assess growth, leaf gas-exchange traits and biochemical parameters. The boron treatments diminished growth, water relations and photosynthetic attributes along with nitrate reductase and carbonic anhydrase activity in the concentration dependent manner whereas, it enhanced lipid peroxidation, electrolyte leakage, accumulation of H(2)O(2) as well as proline, and various antioxidant enzymes in the leaves of mung bean which were more pronounced at higher concentrations of boron. However, the follow-up application of 28-homobrassinolide to the boron stressed plants improved growth, water relations and photosynthesis and further enhanced the various antioxidant enzymes viz. catalase, peroxidase and superoxide dismutase and content of proline. The elevated level of antioxidant enzymes as well as proline could have conferred tolerance to the B-stressed plants resulting in improved growth, water relations and photosynthetic attributes. Copyright © 2011 Elsevier Ltd. All rights reserved.

  9. Biochemical Changes under Chromium Stress on Germinating Seedlings of Vigna radiata

    Directory of Open Access Journals (Sweden)

    Bhavin SUTHAR

    2014-03-01

    Full Text Available Hexavalant chromium is considered the most toxic form because of its high solubility in water. Cr is known to induce production of elevated concentration of reactive oxygen species (ROS resulted in macromolecule damage. Plants are having unique mechanisms to overcome ROS induced damage by accumulation of proline, ascorbate and glutathione and increasing the activities of antioxidant enzymes such as superoxide dismutase (SOD, catalase (CAT, glutathione reductase (GR, and ascorbate peroxidaes (APX, peroxidise (POX. In the present investigation effects of chromium on seed germination of Mung bean (Vigna radiata 'Gujarat Mung-4’ were studied. Seeds were treated with different Cr concentrations (50, 100, 150 and 200 4M for seven days. On 7th day root and shoot length was measured and activities of antioxidant enzyme SOD, APX, POX, CAT and GR were checked along with protein, proline and lipid peroxidation. It was observed that there is gradual decrease in shoot and root length with respect to the increase in Cr concentration. Level of lipid peroxidation significantly increased along with proline and antioxidant enzyme activity at higher Cr concentration. Lipid peroxidation is an indication of membrane damage due to elevated production of reactive oxygen species (ROS. To combat oxidative damage by ROS antioxidant enzyme activity increased significantly, which indicates that antioxidant enzymes (SOD, CAT, APX and GR play a crucial role during Cr stress during germination of V. radiata.

  10. Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata

    Directory of Open Access Journals (Sweden)

    Hien P. Nguyen

    2017-12-01

    Full Text Available The establishment of a root nodule symbiosis between a leguminous plant and a rhizobium requires complex molecular interactions between the two partners. Compatible interactions lead to the formation of nitrogen-fixing nodules, however, some legumes exhibit incompatibility with specific rhizobial strains and restrict nodulation by the strains. Bradyrhizobium elkanii USDA61 is incompatible with mung bean (Vigna radiata cv. KPS1 and soybean cultivars carrying the Rj4 allele. Here, we explored genetic loci in USDA61 that determine incompatibility with V. radiata KPS1. We identified five novel B. elkanii genes that contribute to this incompatibility. Four of these genes also control incompatibility with soybean cultivars carrying the Rj4 allele, suggesting that a common mechanism underlies nodulation restriction in both legumes. The fifth gene encodes a hypothetical protein that contains a tts box in its promoter region. The tts box is conserved in genes encoding the type III secretion system (T3SS, which is known for its delivery of virulence effectors by pathogenic bacteria. These findings revealed both common and unique genes that are involved in the incompatibility of B. elkanii with mung bean and soybean. Of particular interest is the novel T3SS-related gene, which causes incompatibility specifically with mung bean cv. KPS1.

  11. Comparison of traditional field retting and Phlebia radiata Cel 26 retting of hemp fibres for fibre-reinforced composites

    DEFF Research Database (Denmark)

    Liu, Ming; Ale, Marcel Tutor; Kołaczkowski, Bartłomiej

    2017-01-01

    Classical field retting and controlled fungal retting of hemp using Phlebia radiata Cel 26 (a mutant with low cellulose degrading ability) were compared with pure pectinase treatment with regard to mechanical properties of the produced fibre/epoxy composites. For field retting a classification...

  12. Leaf area index, biomass carbon and growth rate of radiata pine genetic types and relationships with LiDAR

    Science.gov (United States)

    Peter N. Beets; Stephen Reutebuch; Mark O. Kimberley; Graeme R. Oliver; Stephen H. Pearce; Robert J. McGaughey

    2011-01-01

    Relationships between discrete-return light detection and ranging (LiDAR) data and radiata pine leaf area index (LAI), stem volume, above ground carbon, and carbon sequestration were developed using 10 plots with directly measured biomass and leaf area data, and 36 plots with modelled carbon data. The plots included a range of genetic types established on north- and...

  13. Susceptibility of Diaphorina citri (Hemiptera: Liviidae) and Its Parasitoid Tamarixia radiata (Hymenoptera: Eulophidae) to Entomopathogenic Fungi under Laboratory Conditions.

    Science.gov (United States)

    Ibarra-Cortés, K H; Guzmán-Franco, A W; González-Hernández, H; Ortega-Arenas, L D; Villanueva-Jiménez, J A; Robles-Bermúdez, A

    2018-02-01

    Diaphorina citri (Kuwayama) is a global pest of citrus that transmits the bacteria associated with the disease, Huanglongbing. Entomopathogenic fungi and the parasitoid Tamarixia radiata (Waterston) are important biological control agents of this pest and likely to interact in D. citri populations. As a basis for interaction studies, we determined the susceptibility of nymphs and adults of D. citri and adults of the parasitoid T. radiata to six fungal isolates from the species Beauveria bassiana s.l. (Bals.-Criv.) Vuill. (isolates B1 and B3), Metarhizium anisopliae s.s. (Metsch.) (Ma129 and Ma65) and Isaria fumosorosea Wize (I2 and Pae). We conducted experiments evaluating infection levels in all three insect groups following inoculation with a series of conidial concentrations (1 × 10 4 -1 × 10 8 conidia mL -1 ). Results showed that D. citri nymphs and T. radiata were more susceptible to fungal isolates than D. citri adults. Overall, B. bassiana and M. anisopliae isolates caused the greatest infection compared with I. fumosorosea isolates in all three groups of insects. Isolates B1 (B. bassiana) and Ma129 (M. anisopliae) infected a greater proportion of adults and nymphs of D. citri, respectively. Both isolates of B. bassiana caused greater infection in T. radiata compared with isolates of the other fungal species. We propose that isolates B1 and Ma129 are the strongest candidates for control of D. citri. Our results represent the first report of entomopathogenic fungi infecting T. radiata, and the basis for future studies to design a biological control programme that uses both agents more efficiently against D. citri populations.

  14. Lethal and Sublethal Impacts of Acaricides on Tamarixia radiata (Hemiptera: Eulophidae), an Important Ectoparasitoid of Diaphorina citri (Hemiptera: Liviidae).

    Science.gov (United States)

    Lira, A C S; Zanardi, O Z; Beloti, V H; Bordini, G P; Yamamoto, P T; Parra, J R P; Carvalho, G A

    2015-10-01

    The use of synthetic acaricides for management of pest mites may alter the efficacy of the ectoparasitoid Tamarixia radiata (Waterston) in biological control of Diaphorina citri Kuwayama, the vector of the bacteria associated with huanglongbing (HLB) in citrus orchards. We evaluated the toxicity of 16 acaricides that are recommended for the control of citrus-pest mites to T. radiata. Acrinathrin, bifenthrin, carbosulfan, and fenpropathrin caused high acute toxicity and were considered harmful (mortality >77%) to T. radiata. Abamectin, diflubenzuron, etoxazole, fenbutatin oxide, fenpyroximate, flufenoxuron, hexythiazox, propargite, spirodiclofen, and sulfur caused low acute toxicity and affected the parasitism rate and emergence rate of adults (F1 generation), and were considered slightly harmful to T. radiata. Dicofol and pyridaben did not affect the survival and action of the ectoparasitoid, and were considered harmless. In addition to its acute toxicity, carbosulfan caused mortality higher than 25% for >30 d after application, and was considered persistent. Acrinathrin, bifenthrin, fenpropathrin, fenpyroximate, propargite, and sulfur caused mortalities over 25% until 24 d after application and were considered moderately persistent; abamectin was slightly persistent, and fenbutatin oxide was short lived. Our results suggest that most acaricides used to control pest mites in citrus affect the density and efficacy of T. radiata in the biological control of D. citri. However, further evaluations are needed in order to determine the effect of these products on this ectoparasitoid under field conditions. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  15. Ecosystem carbon stocks in Pinus palustris forests

    Science.gov (United States)

    Lisa Samuelson; Tom Stokes; John R. Butnor; Kurt H. Johnsen; Carlos A. Gonzalez-Benecke; Pete Anderson; Jason Jackson; Lorenzo Ferrari; Tim A. Martin; Wendell P. Cropper

    2014-01-01

    Longleaf pine (Pinus palustris Mill.) restoration in the southeastern United States offers opportunities for carbon (C) sequestration. Ecosystem C stocks are not well understood in longleaf pine forests, which are typically of low density and maintained by prescribed fire. The objectives of this research were to develop allometric equations for...

  16. The extractives of Pinus pinaster wood

    Science.gov (United States)

    Richard W. Hemingway; W. E. Hillis; L. S. Lau

    1973-01-01

    The extractives in Pinus pinaster wood grown in South Australia were examined as part of an assessment of the suitability of this wood for manufacture of absorbent tissues from bisulphite pulps. The average petroleum solubility of the wood was 2.0% but the amount and composition of the petroleum extract varied widely depending upon the age of the...

  17. Silvical characteristics of pitch pine (Pinus rigida)

    Science.gov (United States)

    S. Little

    1959-01-01

    Pitch pine (Pinus rigida Mill.) grows over a wide geographical range - from central Maine to New York and extreme southeastern Ontario, south to Virginia and southern Ohio, and in the mountains to eastern Tennessee, northern Georgia, and western South Carolina. Because it grows mostly on the poorer soils, its distribution is spotty.

  18. Oviposition behavior and survival of Tamarixia radiata (Hymenoptera: Eulophidae), an ectoparasitoid of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Liviidae), on hosts exposed to an entomopathogenic fungus

    Science.gov (United States)

    Antagonistic interactions between the nymphal parasitoid, Tamarixia radiata Waterston (Hymenoptera: Eulophidae), and the ARSEF 3581 isolate of the entomopathogenic fungus, Isaria fumosorosea Wize (Hypocreales: Cordycipitaceae) could disrupt biological control of the Asian citrus psyllid, Diaphorina ...

  19. DISEÑO DE UN MICROSISTEMA PROGRAMABLE PARA EFECTOS DE AUDIO DIGITAL USANDO FPGAS

    Directory of Open Access Journals (Sweden)

    John Michael Espinosa Durán

    Full Text Available Este artículo describe el diseño de un microsistema programable para el procesamiento de efectos de audio digital implementado en un FPGA. El microsistema es diseñado usando un procesador de propósito específico y reconfigurable, un banco de RAMs y una interfaz gráfica de usuario basada en una pantalla táctil LCD. El procesador es diseñado usando 15 efectos de audio basados en retardos y procesamiento en el dominio dinámico y de la frecuencia. Los efectos son diseñados usando Megafunciones y el compilador FIR de Quartus II, son simulados en Simulink5 usando DSP Builder6, y son configurados utilizando una interfaz gráfica de usuario. El microsistema programable es implementado en el sistema de desarrollo DE2-70, y su funcionamiento es verificado usando un reproductor MP3 y un parlante. Adicionalmente, el microsistema permite la generación de efectos con alta fidelidad usando una tasa de muestreo máxima de 195.62 MSPS, y puede ser embebido en un SoC.

  20. Conserved Epigenetic Mechanisms Could Play a Key Role in Regulation of Photosynthesis and Development-Related Genes during Needle Development of Pinus radiata.

    Science.gov (United States)

    Valledor, Luis; Pascual, Jesús; Meijón, Mónica; Escandón, Mónica; Cañal, María Jesús

    2015-01-01

    Needle maturation is a complex process that involves cell growth, differentiation and tissue remodelling towards the acquisition of full physiological competence. Leaf induction mechanisms are well known; however, those underlying the acquisition of physiological competence are still poorly understood, especially in conifers. We studied the specific epigenetic regulation of genes defining organ function (PrRBCS and PrRBCA) and competence and stress response (PrCSDP2 and PrSHMT4) during three stages of needle development and one de-differentiated control. Gene-specific changes in DNA methylation and histone were analysed by bisulfite sequencing and chromatin immunoprecipitation (ChIP). The expression of PrRBCA and PrRBCS increased during needle maturation and was associated with the progressive loss of H3K9me3, H3K27me3 and the increase in AcH4. The maturation-related silencing of PrSHMT4 was correlated with increased H3K9me3 levels, and the repression of PrCSDP2, to the interplay between AcH4, H3K27me3, H3K9me3 and specific DNA methylation. The employ of HAT and HDAC inhibitors led to a further determination of the role of histone acetylation in the regulation of our target genes. The integration of these results with high-throughput analyses in Arabidopsis thaliana and Populus trichocarpa suggests that the specific epigenetic mechanisms that regulate photosynthetic genes are conserved between the analysed species.

  1. Conserved Epigenetic Mechanisms Could Play a Key Role in Regulation of Photosynthesis and Development-Related Genes during Needle Development of Pinus radiata.

    Directory of Open Access Journals (Sweden)

    Luis Valledor

    Full Text Available Needle maturation is a complex process that involves cell growth, differentiation and tissue remodelling towards the acquisition of full physiological competence. Leaf induction mechanisms are well known; however, those underlying the acquisition of physiological competence are still poorly understood, especially in conifers. We studied the specific epigenetic regulation of genes defining organ function (PrRBCS and PrRBCA and competence and stress response (PrCSDP2 and PrSHMT4 during three stages of needle development and one de-differentiated control. Gene-specific changes in DNA methylation and histone were analysed by bisulfite sequencing and chromatin immunoprecipitation (ChIP. The expression of PrRBCA and PrRBCS increased during needle maturation and was associated with the progressive loss of H3K9me3, H3K27me3 and the increase in AcH4. The maturation-related silencing of PrSHMT4 was correlated with increased H3K9me3 levels, and the repression of PrCSDP2, to the interplay between AcH4, H3K27me3, H3K9me3 and specific DNA methylation. The employ of HAT and HDAC inhibitors led to a further determination of the role of histone acetylation in the regulation of our target genes. The integration of these results with high-throughput analyses in Arabidopsis thaliana and Populus trichocarpa suggests that the specific epigenetic mechanisms that regulate photosynthetic genes are conserved between the analysed species.

  2. Variations in DNA methylation, acetylated histone H4, and methylated histone H3 during Pinus radiata needle maturation in relation to the loss of in vitro organogenic capability.

    Science.gov (United States)

    Valledor, Luis; Meijón, Mónica; Hasbún, Rodrigo; Jesús Cañal, Maria; Rodríguez, Roberto

    2010-03-15

    Needle differentiation is a very complex process associated with the formation of a mature photosynthetic organ. From meristem differentiation to leaf maturation, gene control must play an important role switching required genes on and off to define tissue functions, with the epigenetic code being one of the main regulation mechanisms. In this work, we examined the connections between the variation in the levels of some epigenetic players (DNA methylation, acetylated histone H4 and histone H3 methylation at Lys 4 and Lys 9) at work during needle maturation. Our results indicate that needle maturation, which is associated with a decrease in organogenic capability, is related to an increase in heterochromatin-related epigenetic markers (high DNA methylation and low acetylated histone H4 levels, and the presence of histone H3 methylated at lys 9). Immunohistochemical analyses also showed that the DNA methylation of palisade parenchyma cell layers during the transition from immature to mature scions is associated with the loss of the capacity to induce adventitious organs. Copyright 2009 Elsevier GmbH. All rights reserved.

  3. Deslignificación de pasta kraft de Pinus radiata con una levadura genéticamente modificada para producir lacasa

    OpenAIRE

    Arana-Cuenca, A.; Yagüe, Susana; Fermiñán, Encarnación

    2010-01-01

    Una posible aplicación biotecnológica de las lacasas fúngicas es el blanqueo de pasta de papel mediante la degradación de la lignina en la pasta cruda. Para ello es necesaria su producción a bajo coste y a concentraciones superiores a las secretadas por los hongos de forma natural. La expresión heteróloga en levaduras es una alternativa para su producción a mayor escala. En el presente trabajo se ha realizado la expresión heteróloga del gen cglcc1, responsable de la producción de lacasa en el...

  4. Whole-tree sap flow responses to soil water and weather variables for Pinus radiata and three indigenous species in a southern afrotemperate forest region

    CSIR Research Space (South Africa)

    Mapeto, T

    2018-03-01

    Full Text Available –plant–atmosphere continuum and facilitate the development of rapid estimation techniques through extrapolation. To quantify and characterise the influence of local environmental conditions on single-tree water use, whole-tree daily sap flow for three indigenous afrotemperate...

  5. Cadmium and copper change root growth and morphology of Pinus pinea and Pinus pinaster seedlings

    Energy Technology Data Exchange (ETDEWEB)

    Arduini, I.; Onnis, A. (Dipart. di Agronomia e Gestione dell' Agro-Ecosistema, Univ. degli Studi Pisa, Pisa (Italy)); Boldbold, D.L. (Forstbotanishces Institut, Univ. Goettingen, Goettingen (Germany))

    1994-01-01

    Heavy metal loads in forest soils have been increasing over time due to atmospheric inputs. Accumulation in the upper soil layers could affect establishment of seedlings and forest regeneration. Mediterranean species show a high initial root development, allowing seedlings to reach the moisture of deeper soil layers. In the present work seedlings of stone pine (Pinus pinea L.) and maritime pine (Pinus pinaster Ait.), were grown in culture solution supplied with 0.0, 0.1, 1 or 5 [mu]M CdSO[sub 4] or with 1 [mu]M CdSO[sub 4] and 1 [mu]M CuSO[sub 4] combined. In both species tap-root elongation was drastically reduced in the 5 [mu]M Cd[sup 2+] and in the (Cd[sup 2+] - Cu[sup 2+]) treatments. A supply of 0.1 or 1 [mu]M Cd[sup 2+] however, enhanced root elongation in Pinus pinea without significantly influencing root elongation in Pinus pinaster. In both species the root density (weight per unit length) and the width of the cortex increased in response to Cd[sup 2+] exposure. In Pinus pinaster the mitotic index decreased at the higher Cd[sup 2+] concentrations and when Cd[sup 2+] and Cu[sup 2+] were combined. The data suggest that cell elongation is more sensitive to Cd[sup 2+] than cell division. The number and length of the lateral roots were also affected by Cd[sup 2+] treatment to a higher degree in Pinus pinaster than in Pinus pinea, reflecting the different Cd-tolerance of the two species. (au)

  6. Utilization of Diamine Oxidase Enzyme from Mung Bean Sprouts (Vigna radiata L) for Histamine biosensors

    Science.gov (United States)

    Karim, Abdul; Wahab, A. W.; Raya, I.; Natsir, H.; Arif, A. R.

    2018-03-01

    This research is aimed to utilize the diamine oxidase enzyme (DAO) which isolated from mung bean sprouts (Vigna radiata L) to develop histamine biosensors based on electode enzyme with the amperometric method (cyclic voltammetry).The DAO enzyme is trapped inside the membrane of chitin-cellulose acetate 2:1 and glutaraldehyde which super imposed on a Pt electrode. Histamine will be oxidized by DAO enzyme to produce aldehydes and H2O2 that acting as electron transfer mediators.The performance of biosensors will be measured at various concentrations of glutaraldehyde, temperature changes and different range of pH. Recently, it has been found that the optimal conditions obtained from the paramaters as follows; at 25% of glutaraldehyde, temperature of 37°C and pH of 7.4. Eventually, the results provided an expectation for applying histamine biosensors in determining the freshness and safety of fish specifically skombroidae families.

  7. Yield and Quality of Mung Bean (Vigna radiata (l. R. Wilczek Seeds Produced in Poland

    Directory of Open Access Journals (Sweden)

    Kamil MISIAK

    2017-11-01

    Full Text Available The aim of the experiment was to do field and laboratory assessments of yield and quality of mung bean (Vigna radiata (L. R. Wilczek seeds cultivated in Western Poland. Mean yield of seeds per plant was higher for common bean (Phaseolus vulgaris L. than for mung one: 13.1 g and 2.58 g, respectively. The mean 1000 mung seeds weight was 50.9 g and their germination – 78 %. Germination capacities of seeds of both beans in the field were similar. Mung beans, compared to common bean, had much smaller seeds, started to bloom later and produced mature seeds later than the latter. Mung bean seeds had more total proteins and Magnesium and Copper than common bean seeds. In Western Poland, production of high quality mung bean seeds was possible.

  8. Biogenic synthesis and spatial distribution of silver nanoparticles in the legume mungbean plant (Vigna radiata L.).

    Science.gov (United States)

    Kumari, Rima; Singh, Jay Shankar; Singh, Devendra Pratap

    2017-01-01

    The present investigation aimed to study the in vivo synthesis of silver nanoparticles (AgNPs) in the legume Vigna radiata. The level of plant metabolites such as total phenolics, lipid, terpenoids, alkaloids and amino acid increased by 65%, 133%, 19%, 67% and 35%, respectively, in AgNO 3 (100 mg L -1 ) treated plants compared to control. Whereas protein and sugar contents in the treated plants were reduced by 38% and 27%, respectively. FTIR analysis of AgNO 3 (20-100 mg L -1 ) treated plants exhibited changes in the IR regions between 3297 and 3363 cm -1 , 1635-1619 cm -1 , 1249-1266 cm -1 and that corresponded to alterations in OH groups of carbohydrates, OH and NH groups of amide I and II regions of protein, when compared with the control. Transmission electron micrographs showed the spatial distribution of AgNPs in the chloroplast, cytoplasmic spaces, vacuolar and nucleolar plant regions. Metal quantification in different tissues of plants exposed to 20-100 mg L -1 AgNO 3 showed about a 22 fold accumulation of Ag in roots as compared to shoots. The phytotoxic parameters such as percent seed germination and shoot elongation remained almost unaltered at low AgNO 3 doses (20-50 mg L -1 ). However, at higher levels of exposure (100 mg L -1 ), the percent seed germination as well as root and shoot elongation exhibited concentration dependent decline. In conclusion, synthesis of AgNPs in V. radiata particularly at lower doses of AgNO 3 , could be used as a sustainable and environmentally safe technology for large scale production of metal nanoparticles. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

  9. Anatomical aspects of the male reproductive system in the bonnet monkey (Macaca radiata).

    Science.gov (United States)

    Prakash, S; Suresh, S; Prithiviraj, E

    2009-04-01

    The normal anatomy of the male reproductive system in Macaca radiata is presented here. The external genitalia consist of a triangular button-shaped glans penis. The corpus cavernosum, and spongiosum form the vascular component of the penis and the baculum or os penis forms the non-vascular erectile component. The baculum is one of the longest in the genus macaques. The scrotal sac is non-pigmented, slightly pendulous, with scattered hairs, faintly corrugated, and does not reach the ischial callosities in the sitting posture. The testicles are ovoid in shape without appendix. Right and left testicular arteries originate at the level of the inter-vertebral disc between T12-L1 and L2-L3, respectively. Seminiferous tubules present mixed stages of spermatogenesis, i.e. single/multistage. The epididymis is crescent shaped, attached to the postero-lateral border of the testis without an appendix. Light microscopic observation revealed a characteristic high columnar epithelium with stereocilia. Clear cells or light cells are seen in the caudal region. The ductus deferens display a lumen lined by pseudo-stratified columnar epithelium separated by concentric layers of smooth muscle cells covered by serosa. The seminal vesicles are pyramidal in shape, prominently projecting above the urinary bladder, and are the largest of the accessory glands, typical of polyandrous primate genera. The prostate is conical in shape. Its base is in contact with the trigone of the bladder. Its posterior surface shows a transverse cleft separating an upper quarter, the cranial lobe, from the lower three-quarters of the gland. Compared with other macaques there are many distinguishing features in M. radiata. Excellent adaptability and spermatogenic efficiency in the laboratory environment makes this animal a good primate model for andrological research.

  10. Lethal and Sublethal Effects of Insecticides Used on Citrus, on the Ectoparasitoid Tamarixia radiata.

    Science.gov (United States)

    Beloti, Vitor Hugo; Alves, Gustavo Rodrigues; Araújo, Diogo Feliciano Dias; Picoli, Mateus Manara; Moral, Rafael de Andrade; Demétrio, Clarice Garcia Borges; Yamamoto, Pedro Takao

    2015-01-01

    Huanglongbing (HLB) is a disease associated with the bacteria "Candidatus Liberibacter spp." and has been devastating citrus orchards around the world. Its management involves control of the insect vector, the Asian citrus psyllid Diaphorina citri Kuwayama. However, the indiscriminate use of chemicals has caused pest outbreaks and eliminated the natural enemies of the vector, such as the parasitoid Tamarixia radiata (Waterston), the main agent for biological control of D. citri. This study assessed the lethal and sublethal effects of insecticides recommended for integrated production of citrus on the parasitoid T. radiata. When adult parasitoids were exposed to residues of 25 insecticides, 20% of them, i.e., gamma-cyhalothrin, etofenprox, azadirachtin, tebufenozide and pyriproxyfen, were considered as harmless (Class 1), 12% as slightly harmful (Class 2), 12% as moderately harmful (Class 3) and 56% as harmful (Class 4), according to the classification proposed by the IOBC/WPRS. Afterward, 14 insecticides (5 harmless and 9 harmful) were sprayed on the parasitoid pupae. Of the 14 insecticides tested, only the organophosphates dimethoate and chlorpyrifos affected the parasitoid emergence. The effects of insecticides on the parasitism capacity of adults exposed to residues of azadirachtin, etofenprox, gamma-cyhalothrin, pyriproxyfen and tebufenozide (harmless) were also evaluated. Tebufenozide and gamma-cyhalothrin affected the parasitism of the F0 generation, but did not affect the emergence of the F1 and F2 generations. Therefore, for an effective IPM program, selective insecticides or harmful pesticides to adult parasitoids could be used in the field, provided that the adults do not occur naturally and the chemical applications do not coincide with parasitoid releases.

  11. Lethal and Sublethal Effects of Insecticides Used on Citrus, on the Ectoparasitoid Tamarixia radiata.

    Directory of Open Access Journals (Sweden)

    Vitor Hugo Beloti

    Full Text Available Huanglongbing (HLB is a disease associated with the bacteria "Candidatus Liberibacter spp." and has been devastating citrus orchards around the world. Its management involves control of the insect vector, the Asian citrus psyllid Diaphorina citri Kuwayama. However, the indiscriminate use of chemicals has caused pest outbreaks and eliminated the natural enemies of the vector, such as the parasitoid Tamarixia radiata (Waterston, the main agent for biological control of D. citri. This study assessed the lethal and sublethal effects of insecticides recommended for integrated production of citrus on the parasitoid T. radiata. When adult parasitoids were exposed to residues of 25 insecticides, 20% of them, i.e., gamma-cyhalothrin, etofenprox, azadirachtin, tebufenozide and pyriproxyfen, were considered as harmless (Class 1, 12% as slightly harmful (Class 2, 12% as moderately harmful (Class 3 and 56% as harmful (Class 4, according to the classification proposed by the IOBC/WPRS. Afterward, 14 insecticides (5 harmless and 9 harmful were sprayed on the parasitoid pupae. Of the 14 insecticides tested, only the organophosphates dimethoate and chlorpyrifos affected the parasitoid emergence. The effects of insecticides on the parasitism capacity of adults exposed to residues of azadirachtin, etofenprox, gamma-cyhalothrin, pyriproxyfen and tebufenozide (harmless were also evaluated. Tebufenozide and gamma-cyhalothrin affected the parasitism of the F0 generation, but did not affect the emergence of the F1 and F2 generations. Therefore, for an effective IPM program, selective insecticides or harmful pesticides to adult parasitoids could be used in the field, provided that the adults do not occur naturally and the chemical applications do not coincide with parasitoid releases.

  12. Improvement of non-key traits in radiata pine breeding programme when long-term economic importance is uncertain.

    Science.gov (United States)

    Li, Yongjun; Dungey, Heidi; Yanchuk, Alvin; Apiolaza, Luis A

    2017-01-01

    Diameter at breast height (DBH), wood density (DEN) and predicted modulus of elasticity (PME) are considered as 'key traits' (KT) in the improvement in radiata pine breeding programmes in New Zealand. Any other traits which are also of interest to radiata pine breeders and forest growers are called 'non-key traits' (NKTs). External resin bleeding (ERB), internal checking (IC), number of heartwood rings (NHR) are three such non-key traits which affect wood quality of radiata pine timber. Economic importance of the KTs and NKTs is hard to define in radiata pine breeding programmes due to long rotation period. Desired-gain index (DGIs) and robust selection were proposed to incorporate NKTs into radiata pine breeding programme in order to deal with the uncertainty of economic importance. Four desired-gain indices A-D were proposed in this study. The desired-gain index A (DGI-A) emphasized growth and led to small decrease in ERB and small increase in IC and NHR. The expected genetic gains of all traits in the desired-gain index B (DGI-B) were in the favourable directions (positive genetic gains in the key traits and negative genetic gains in the non-key traits). The desired-gain index C (DGI-C) placed emphasis on wood density, leading to favourable genetic gain in the NKTs but reduced genetic gains for DBH and PME. The desired-gain index D (DGI-D) exerted a bit more emphasis on the non-key traits, leading large favourable reduction in the non-key traits and lower increase in the key traits compared with the other DGIs. When selecting both the key traits and the non-key traits, the average EBVs of six traits were all in the same directions as the expected genetic gains except for DBH in the DGI-D. When the key traits were measured and selected, internal checking always had a negative (favourable) genetic gain but ERB and NHR had unfavourable genetic gain in the most of time. After removing some individuals with high sensitivity to the change of economic weights, robust

  13. Improvement of non-key traits in radiata pine breeding programme when long-term economic importance is uncertain.

    Directory of Open Access Journals (Sweden)

    Yongjun Li

    Full Text Available Diameter at breast height (DBH, wood density (DEN and predicted modulus of elasticity (PME are considered as 'key traits' (KT in the improvement in radiata pine breeding programmes in New Zealand. Any other traits which are also of interest to radiata pine breeders and forest growers are called 'non-key traits' (NKTs. External resin bleeding (ERB, internal checking (IC, number of heartwood rings (NHR are three such non-key traits which affect wood quality of radiata pine timber. Economic importance of the KTs and NKTs is hard to define in radiata pine breeding programmes due to long rotation period. Desired-gain index (DGIs and robust selection were proposed to incorporate NKTs into radiata pine breeding programme in order to deal with the uncertainty of economic importance. Four desired-gain indices A-D were proposed in this study. The desired-gain index A (DGI-A emphasized growth and led to small decrease in ERB and small increase in IC and NHR. The expected genetic gains of all traits in the desired-gain index B (DGI-B were in the favourable directions (positive genetic gains in the key traits and negative genetic gains in the non-key traits. The desired-gain index C (DGI-C placed emphasis on wood density, leading to favourable genetic gain in the NKTs but reduced genetic gains for DBH and PME. The desired-gain index D (DGI-D exerted a bit more emphasis on the non-key traits, leading large favourable reduction in the non-key traits and lower increase in the key traits compared with the other DGIs. When selecting both the key traits and the non-key traits, the average EBVs of six traits were all in the same directions as the expected genetic gains except for DBH in the DGI-D. When the key traits were measured and selected, internal checking always had a negative (favourable genetic gain but ERB and NHR had unfavourable genetic gain in the most of time. After removing some individuals with high sensitivity to the change of economic weights

  14. Vigna radiata

    Indian Academy of Sciences (India)

    Table 2. Cluster mean for different traits. Characters/ Days to 50% Days to Plant height Plant yield. 100-seed. Pods per Pods per Pod length Seeds per clusters flowering maturity. (cm). (gm) weight (gm) plant cluster. (cm) pod. Cluster I. 34.00. 6.20. 29.64. 4.89. 4.71. 13.15. 3.29. 6.69. 8.74. Cluster II. 35.17. 61.00. 31.29. 5.26.

  15. SISTEMA DE MODELAGEM DE FIGURAS TRIDIMENSIONAIS USANDO RECONHECIMENTO DE VOZ

    Directory of Open Access Journals (Sweden)

    Kauan Cristiano De Souza

    2017-01-01

    Full Text Available Atualmente o mercado de Sistemas de Desenho auxiliado por Computador (CAD, sofre extrema carência de processos automatizados de reconhecimento de fala. O surgimento de tecnologias de automação facilita no desenvolvimento de aplicações capazes de auxiliar nestes processos. Baseando-se nestas afirmações construiu-se um modelo utilizando tecnologias capazes de transformar fala em sinais digitais, sendo possível a interpretação por sofisticados computadores. Observando ausência de tais ferramentas, o presente trabalho busca uma proposta capaz de acrescentar mecanismos os quais possibilitem a criação de modelos tridimensionais usando tecnologias como servidores HTTP, dispositivos moveis, linguagens de programação Java e PHP, Computação Gráfica e linguagem de interpretação por blocos. Os resultados obtidos na confecção do presente trabalho corroboram com as necessidades atuais representadas na sociedade propondo sustentabilidade, acessibilidade, portabilidade e facilidade de comunicação.

  16. [Comparison of chemical components of essential oils in needles of Pinus massoniana Lamb and Pinus elliottottii Engelm from Guangxi].

    Science.gov (United States)

    Shen, Changmao; Duan, Wengui; Cen, Bo; Tan, Jianhui

    2006-11-01

    Essential oils were extracted by steam distillation from the needles of Pinus massoniana Lamb and Pinus elliottottii Engelm grown in Guangxi. Various factors such as pine needle dosage and extraction time which may influence the oil yield were investigated. The optimum conditions were found to be as follows: pine needle dosage 700 g, extraction time 5 h. The essential oil yields from the needles of Pinus massoniana Lamb and Pinus elliottottii Engelm were 0.45% and 0.19%, respectively. Moreover, the chemical compositions of the essential oils were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Sixty four components in the essential oil from needle of Pinus massoniana Lamb were separated and twenty of them (98.59%) were identified while seventy three components in the essential oil from needle of Pinus elliottottii Engelm were separated and twenty nine of them (94.23%) were identified. Generally, the compositions of the essential oils from needles of the two varieties were similar but the contents of some compounds differed greatly. Especially, the content of alpha-pinene in the essential oils from Pinus massoniana Lamb needles was 2.6 times as that from Pinus elliottottii Engelm needles, but the content of beta-pinene was less than the latter. Mono- and sesquiterpenes were the main composition of the essential oils from Pinus massoniana Lamb and Pinus elliottottii Engelm needles.

  17. Comparative mapping in Pinus: sugar pine (Pinus lambertiana Dougl.) and loblolly pine (Pinus taeda L.).Tree Genet Genomes 7:457-468

    Science.gov (United States)

    Kathleen D. Jermstad; Andrew J. Eckert; Jill L. Wegrzyn; Annette Delfino-Mix; Dean A Davis; Deems C. Burton; David B. Neale

    2011-01-01

    The majority of genomic research in conifers has been conducted in the Pinus subgenus Pinus mostly due to the high economic importance of the species within this taxon. Genetic maps have been constructed for several of these pines and comparative mapping analyses have consistently revealed notable synteny. In contrast,...

  18. Modeling natural regeneration biomass of Pinus stand

    Directory of Open Access Journals (Sweden)

    Rafael Cubas

    2016-09-01

    Full Text Available Reliable biomass data are very important in the evaluation of ecosystems, and help in understanding the contribution of forests in climate change. Variables that describe the size of the tree, like diameter and height are directly associated with biomass, which allows the use of regression models to estimate this element. Therefore, this study aimed to estimate by regression models, the biomass of different compartments of natural regeneration of trees of a Pinus taeda L. stand. The data were obtained through direct destructive method, using 100 randomly selected trees in the understory of a stand of Pinus taeda. We analyzed three arithmetical models, three logarithmic and two models developed by Stepwise process. Logarithmic equations developed by Stepwise procedure showed the best estimates of total and stems biomass. However, for needles and twigs compartments the best adjust was observed with Husch model and for root biomass Berkhout model proved to be the most suitable.

  19. Foliar fungi of Scots pine (Pinus sylvestris)

    OpenAIRE

    Millberg, Hanna

    2015-01-01

    Scots pine (Pinus sylvestris) is an ecologically and economically important tree species in Fennoscandia. Scots pine needles host a variety of fungi, some with the potential to profoundly influence their host. These fungi can have beneficial or detrimental effects with important implications for both forest health and primary production. In this thesis, the foliar fungi of Scots pine needles were investigated with the aim of exploring spatial and temporal patterns, and development with needle...

  20. Eficiência dos herbicidas oxadiazil, oxadiazon, oxyfluorfen e Imazapyr sobre a cultura de pinus Efficiency of the oxadiazil, oxadiazon, oxyfluorfen and imazapyr herbicides on Pinus cropping

    Directory of Open Access Journals (Sweden)

    Wilson da Silva

    1999-08-01

    Full Text Available Avaliou-se a seletividade de alguns herbicidas para mudas de Pinus caribaea var. Hondurensis e suas eficiências no controle de plantas daninhas. As mudas com 20 cm de altura, produzidas em tubetes, foram transplantadas num espaçamento de 2,0 m x 2,0 m, em parcelas de quatro fileiras com 10,0 m de comprimento. Os tratamentos utilizados foram oxadiazil (600; 800 e 1000 g ha-1, oxadiazon (1140; 1520 e 1900 g ha-1, oxyfluorfen (720 g ha-1, imazapyr (250 g ha-1 e testemunhas (capinada e sem capina, dispostos em blocos ao acaso, com quatro repetições. Os herbicidas foram aplicados sete dias após o transplante, usando-se pulverizador costal, pressurizado com CO2, calibrado para 200 L ha- 1 de calda. As principais plantas daninhas com maior infestação foram: Brachiaria plantaginea e Ipomoea grandifolia e com menor infestação: Galinsoga parviflora e Bidens pilosa. O oxadiazil apresentou excelente controle de B. plantaginea, I. grandifolia e G. parviflora, não sendo eficiente para controle de B. pilosa, embora proporcionasse controle superior ao observado pelo oxadiazon. O oxadiazon foi eficiente até 45 dias após o tratamento para B. plantaginea, G. parviflora e até 90 dias para I. grandifolia. Tanto o oxyfluorfen quanto o imazapyr apresentaram excelente controle das plantas daninhas. De todos os herbicidas avaliados, apenas o imazapyr não deve ser recomendado para aplicação sobre o dossel de Pinus caribaea var. Hondurensis, em face da toxicidade provocada.The selectivity of some herbicides were evaluated on Pinus caribaea var. Hondurensis seedlings as well as these herbicide efficiencies in the weed control. When the tubule-produced seedlings were 20 cm height they were transplanted at 2,0 m x 2,0 m spacings in fourrows plots with 10,0 m length. The treatments with oxadiazil (600; 800 and 1000 g ha-1, oxadiazon (1140; 1520 and 1900 g ha-1, oxyfluorfen (720 g ha-1, imazpyr (250 g ha-1 and controls (weeded and without weeding were disposed

  1. La corona radiata de Helios-Sol como símbolo de poder en la Cultura Visual Romana

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    Jorge Tomás García

    2017-12-01

    Full Text Available El presente trabajo pretende analizar la presencia del motivo iconográfico de la corona radiata en la cultura visual romana como símbolo de poder. Para ello, analizaremos la figura mitológica de Helios, y sus múltiples variantes en las fuentes clásicas, especialmente aquellas que más la relacionan con la divinidad del Sol en el mundo romano. Las principales categorías de interpretación de la iconografía del Sol en la cultura visual romana enriquecen las variantes iconológicas de la presencia de la corona radiata. Así, pretendemos analizar la naturaleza real o simbólica de este atributo iconográfico tan presente desde la época de Augusto como símbolo de poder y luz ligado a la realía imperial. This article aims to analyze the presence of the iconographic motif of the corona radiata in the Roman visual culture as a symbol of power. For this, we will analyze the mythological figure of Helios, and its multiple variants in the classical sources, especially those that relate more to the divinity of the Sun in the Roman world. e main categories of interpretation of the Sun's iconography in the Roman visual culture enrich the iconological variants of the presence of the corona radiata. us, we intend to analyze the real or symbolic nature of this iconographic attribute so present since the time of Augustus as a symbol of power and light linked to the imperial realia.

  2. Contenido de carotenos en el follaje de Pinus caribaea Morelet y Pinus tropicalis Morelet

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    Rolando Quert Álvarez

    1997-08-01

    Full Text Available Se realizó un análisis del material vegetal de las especies Pinus caribaea Morelet y Pinus tropicalis Morelet, con el objetivo de determinar su contenido de caroteno tomando como base las condiciones y tiempo de exposición del follaje de las especies objeto de estudio al sol y a la sombra, y teniendo en cuenta la extracción del aceite esencias como factores influyentes en la variación de las concentraciones de caroteno. Para determinar el contenido de caroteno se tomaron muestras del follaje entre 1 y 20 d, expuesto a las condiciones de trabajo en intervalos de 1, 3, 6, 10 y 20 d, tanto antes como después de extraer el aceite esencial. Los resultados obtenidos para ambas especies demostraron que el follaje expuesto a la sombra contiene un mayor porcentaje de caroteno que el expuesto al sol, como era de esperar; el tiempo de exposición influye significativamente en este contenido, así como la extracción del aceite esencial que aumenta el contenido de caroteno; los valores máximos fueron de 130,7 y 157,2 mg/kg de follaje y los mínimos de 55,3 y 57,2 mg/kg de follaje para Pinus caribaea Morelet y Pinus tropicalis Morelet respectivamente.An analysis of the vegetable material from the species Pinus cariabaea Morelet and Pinus tropicalis Morelet was carried out and their content of carotene was determined taking as the basis the conditions and time of exposure of the foliage of the species studied to the sun and shade, and also taking into account the extraction of the essential oil as factors influencing on the variation of carotene concentrations. For the determination of the carotene content, samples of the foliage between 1 and 20 exposed to working conditions at intervals of 1, 3, 6, 10 and 20 d were taken, both before and after the extraction of the essential oil. Results obteined from both species showed that the foliage exposed to shade contains a higher percentage of carotene than the one exposed to the sun, as it was expected to

  3. Aulas-laboratorios de bajo costo, usando TIC

    Directory of Open Access Journals (Sweden)

    Silvia E. Calderón

    2015-01-01

    Full Text Available En este trabajo se presentan los resultados de una propuesta educativa orientada a promover el desarrollo de un pensamiento critico y un mayor interes por las ciencias experimentales. Con este fin desarrollamos propuestas de proyectos educativos susceptibles de ser destinadas a las aulas y laboratorios de las escuelas secundarias y primeros anos de la universidad, que resaltan los aspectos metodologicos de las ciencias. Aqui, realizamos una compilacion de varios proyectos, que ilustran formas de incorporar las tecnologias de la informacion y la comunicacion (TIC en diversos experimentos de ciencias, muchos de ellos publicados individualmente anteriormente, y que en conjunto se pueden utilizar para implementar un aula-laboratorio de bajo costo. Con TIC hacemos referencia a la convergencia de computadoras, sistemas audiovisuales, Internet, telefonia, y diversos equipos que se integran con algunos de ellos. Los proyectos intentan integrar areas como fisica, matematica, quimica, informatica, arte, etc. y apuntan a que los estudiantes puedan responder a las preguntas: .Como sabemos esto?, .Por que creemos en aquello? Preguntas que ilustran la naturaleza del pensamiento cientifico. Nuestra contribucion mas significativa es haber desarrollado ≪aulas-laboratorios≫ de muy bajo costo, usando TIC. Con el advenimiento de programas como ≪Una Laptop por Nino≫ que se estan implementando en varios paises de Latinoamerica, resulta oportuno utilizar este recurso como base para generar laboratorios de bajo costo, que creemos pueden ser una herramienta util para mejorar el aprendizaje de las ciencias, incentivar vocaciones y contribuir a desarrollar un pensamiento critico, a la par de desarrollar habilidades con el uso de las TIC que pueden ser de utilidad en diversos ambitos academicos y laborales.

  4. Needle Terpenes as Chemotaxonomic Markers in Pinus: Subsections Pinus and Pinaster.

    Science.gov (United States)

    Mitić, Zorica S; Jovanović, Snežana Č; Zlatković, Bojan K; Nikolić, Biljana M; Stojanović, Gordana S; Marin, Petar D

    2017-05-01

    Chemical compositions of needle essential oils of 27 taxa from the section Pinus, including 20 and 7 taxa of the subsections Pinus and Pinaster, respectively, were compared in order to determine chemotaxonomic significance of terpenes at infrageneric level. According to analysis of variance, six out of 31 studied terpene characters were characterized by a high level of significance, indicating statistically significant difference between the examined subsections. Agglomerative hierarchical cluster analysis has shown separation of eight groups, where representatives of subsect. Pinaster were distributed within the first seven groups on the dendrogram together with P. nigra subsp. laricio and P. merkusii from the subsect. Pinus. On the other hand, the eighth group included the majority of the members of subsect. Pinus. Our findings, based on terpene characters, complement those obtained from morphological, biochemical, and molecular parameters studied over the past two decades. In addition, results presented in this article confirmed that terpenes are good markers at infrageneric level. © 2017 Wiley-VHCA AG, Zurich, Switzerland.

  5. Growth and dynamic modulus of elasticity of Pinus patula × Pinus ...

    African Journals Online (AJOL)

    Field establishment of South Africa's most important commercial pine species, Pinus patula, is severely hampered by the pitch canker fungus, Fusarium circinatum. Importantly, hybrids between P. patula and other pine species tolerant to the pitch canker fungus, such as P. tecunumanii and P. oocarpa, have been identified ...

  6. The flexural properties of young Pinus elliottii × Pinus caribaea var ...

    African Journals Online (AJOL)

    The objectives of this study were to determine the bending strength and stiffness properties of young Pinus elliottii × P. caribaea var. hondurensis timber from the Southern Cape, South Africa, and to evaluate the predictability of these properties from acoustic measurements on standing trees, logs and their sawn boards.

  7. Rust resistance in seedling families of Pinus albicaulis and Pinus strobiformis and implications for restoration

    Science.gov (United States)

    R. A. Sniezko; A. Kegley; R. Danchok; J. Hamlin; J. Hill; D. Conklin

    2011-01-01

    Infection and mortality levels from Cronartium ribicola, the fungus causing white pine blister rust, are very high in parts of the geographic range of Pinus albicaulis (whitebark pine) and P. strobiformis (Southwestern white pine). Genetic resistance to this non-native fungus will be one of the key factors in maintaining or restoring populations of these species in...

  8. Growth and provenance variation of Pinus caribaea var ...

    African Journals Online (AJOL)

    CAMCORE has visited 33 populations of Pinus caribaea var. hondurensis in Belize, Guatemala, Honduras, El Salvador, Nicaragua, and Quintana Roo, Mexico. Seed collections have been made in 29 provenances from 1, 325 mother trees. A total of 21 provenances and sources of Pinus caribaea var. hondurensis were ...

  9. Germination and early seedling growth of Pinus densata Mast. provenances

    Science.gov (United States)

    Yulan Xu; Nianhui Cai; Bin He; Ruili Zhang; Wei Zhao; Jianfeng Mao; Anan Duan; Yue Li; Keith Woeste

    2016-01-01

    We studied seed germination and early seedling growth of Pinus densata to explore the range of variability within the species and to inform afforestation practices. Phenotypes were evaluated at a forest tree nursery under conditions that support Pinus yunnanensis, one of the presumed parental species of P. densata...

  10. Critical water stress levels in Pinus patula seedlings and their ...

    African Journals Online (AJOL)

    Critical water stress levels in Pinus patula seedlings and their relation to measures of seedling morphology. ... Southern Forests: a Journal of Forest Science ... A pot trial was implemented to determine the effect of soil water stress following transplanting on shoot water potential and stomatal conductance of Pinus patula ...

  11. Effect on nursery and field performance of Pinus patula seedlings ...

    African Journals Online (AJOL)

    Fusarium circinatum is an important fungal pathogen of Pinus species. In South Africa, it is the most significant pathogen of Pinus patula seedlings in forestry nurseries where it presents a substantial constraint to productivity and can continue to cause mortality in-field for up to two years after establishment. This study ...

  12. Morphological evaluation of the Pinus kesiya complex (Pinaceae)

    Czech Academy of Sciences Publication Activity Database

    Businský, R.; Frantík, Tomáš; Vít, Petr

    2014-01-01

    Roč. 300, č. 2 (2014), s. 273-285 ISSN 0378-2697 Institutional support: RVO:67985939 Keywords : morphological var iation * Pinus densata ssp. tibetica * Pinus kesiya complex Subject RIV: EF - Botanics Impact factor: 1.422, year: 2014

  13. An evaluation of wood properties of Pinus caribeae (Morelet) in ...

    African Journals Online (AJOL)

    This paper examines the effects of within tree variation on wood density, ring width and anisotropic shrinkage of Pinus caribeae (Morelet) among tree partitions in Oluwa pine plantation. Five 15-year old Pinus caribeae (Morelet) in three partitions were randomly selected from the plantation and felled for the study. Wood ...

  14. Host range testing of Tamarixia radiata (Hymenoptera: Eulophidae) sourced from the Punjab of Pakistan for classical biological control of Diaphorina citri (Hemiptera: Liviidae: Euphyllurinae: Diaphorinini) in California.

    Science.gov (United States)

    Hoddle, Mark S; Pandey, Raju

    2014-02-01

    ABSTRACT Tests evaluating the host range of Tamarixia radiata (Waterson) (Hymenoptera: Eulophidae), a parasitoid of the pestiferous Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), sourced from the Punjab of Pakistan, were conducted in quarantine at the University of California, Riverside, CA. Seven nontarget psyllid species (five native and two self-introduced species) representing five families were exposed to T radiata under the following three different exposure scenarios: 1) sequential no-choice tests, 2) static no-choice tests, and 3) choice tests. Nontarget species were selected for testing based on the following criteria: 1) taxonomic relatedness to the target, D. citri; 2) native psyllids inhabiting native host plants related to citrus that could release volatiles attractive to T. radiata; 3) native psyllids with a high probability of occurrence in native vegetation surrounding commercial citrus groves that could be encountered by T. radiata emigrating from D. citri-infested citrus orchards; 4) a common native pest psyllid species; and 5) a beneficial psyllid attacking a noxious weed. The results of host range testing were unambiguous; T radiata exhibited a narrow host range and high host specificity, with just one species of nontarget psyllid, the abundant native pest Bactericera cockerelli Sulc, being parasitized at low levels (citri poses negligible environmental risk.

  15. Identificación de sistemas no lineales usando series Volterra – Laguerre

    OpenAIRE

    Medina Ramos, Carlos Celestino; Medina Ramos, Carlos Celestino

    2011-01-01

    Este trabajo de Tesis está enfocado en la identificación de sistemas no lineales de modelo dinámico no conocido, adicionalmente y en base a los resultados obtenidos, se propone la aplicación del sistema de Control Predictivo no Lineal Basado en Modelos, NMPC, usando el algoritmo de la Matriz Dinámica de Control no Lineal, NDMC. El primer objetivo de este trabajo consiste en implementar una metodología para la identificación de sistemas no lineales usando series de Volterra truncadas; proye...

  16. Connectivity of the habitat-forming kelp, Ecklonia radiata within and among estuaries and open coast.

    Science.gov (United States)

    Coleman, Melinda A

    2013-01-01

    With marine protected areas being established worldwide there is a pressing need to understand how the physical setting in which these areas are placed influences patterns of dispersal and connectivity of important marine organisms. This is particularly critical for dynamic and complex nearshore marine environments where patterns of genetic structure of organisms are often chaotic and uncoupled from broad scale physical processes. This study determines the influence of habitat heterogeneity (presence of estuaries) on patterns of genetic structure and connectivity of the common kelp, Ecklonia radiata. There was no genetic differentiation of kelp between estuaries and the open coast and the presence of estuaries did not increase genetic differentiation among open coast populations. Similarly, there were no differences in level of inbreeding or genetic diversity between estuarine and open coast populations. The presence of large estuaries along rocky coastlines does not appear to influence genetic structure of this kelp and factors other than physical heterogeneity of habitat are likely more important determinants of regional connectivity. Marine reserves are currently lacking in this bioregion and may be designated in the future. Knowledge of the factors that influence important habitat forming organisms such as kelp contribute to informed and effective marine protected area design and conservation initiatives to maintain resilience of important marine habitats.

  17. The AVRDC - The World Vegetable Center mungbean (Vigna radiata) core and mini core collections.

    Science.gov (United States)

    Schafleitner, Roland; Nair, Ramakrishnan Madhavan; Rathore, Abhishek; Wang, Yen-wei; Lin, Chen-yu; Chu, Shu-hui; Lin, Pin-yun; Chang, Jian-Cheng; Ebert, Andreas W

    2015-04-29

    Large ex situ germplasm collections generally harbor a wide range of crop diversity. AVRDC--The World Vegetable Center is holding in trust the world's second largest mungbean (Vigna radiata) germplasm collection with more than 6,700 accessions. Screening large collections for traits of interest is laborious and expensive. To enhance the access of breeders to the diversity of the crop, mungbean core and mini core collections have been established. The core collection of 1,481 entries has been built by random selection of 20% of the accessions after geographical stratification and subsequent cluster analysis of eight phenotypic descriptors in the whole collection. Summary statistics, especially the low differences of means, equal variance of the traits in both the whole and core collection and the visual inspection of quantile-quantile plots comparing the variation of phenotypic traits present in both collections indicated that the core collection well represented the pattern of diversity of the whole collection. The core collection was genotyped with 20 simple sequence repeat markers and a mini core set of 289 accessions was selected, which depicted the allele and genotype diversity of the core collection. The mungbean core and mini core collections plus their phenotypic and genotypic data are available for distribution to breeders. It is expected that these collections will enhance the access to biodiverse mungbean germplasm for breeding.

  18. Cytotoxic and Antimalarial Amaryllidaceae Alkaloids from the Bulbs of Lycoris radiata

    Directory of Open Access Journals (Sweden)

    Bin Hao

    2013-02-01

    Full Text Available Phytochemical investigation of the 80% ethanol extract of the bulbs of Lycoris radiata resulted in the isolation of five new Amaryllidaceae alkaloids: (+-5,6-dehydrolycorine (1, (+-3α,6β-diacetyl-bulbispermine (2, (+-3α-hydroxy-6β-acetyl- bulbispermine (3, (+-8,9-methylenedioxylhomolycorine-N-oxide (5, and 5,6-dihydro-5- methyl-2-hydroxyphenanthridine (7, together with two known compounds, (+-3α-methoxy- 6β-acetylbulbispermine (4 and (+-homolycorine- N-oxide (6. Structural elucidation of all the compounds were performed by spectral methods such as 1D and 2D (1H-1H COSY, HMQC, and HMBC NMR spectroscopy, in addition to high resolution mass spectrometry. Alkaloid 1 showed potent cytotoxicity against astrocytoma and glioma cell lines (CCF-STTG1, CHG-5, SHG-44, and U251, as well as HL-60, SMMC-7721, and W480 cell lines with IC50 values of 9.4–11.6 μM. Additonally, compound 1 exhibited antimalarial activity with IC50 values of 2.3 μM for D-6 strain and 1.9 μM for W-2 strain of Plasmodium falciparum.

  19. Pretreatment of radiata pine using two white rot fungal strains Stereum hirsutum and Trametes versicolor

    International Nuclear Information System (INIS)

    Shirkavand, Ehsan; Baroutian, Saeid; Gapes, Daniel J.; Young, Brent R.

    2017-01-01

    Highlights: • Fungal pretreatment by two New Zealand native white rot fungi was proposed. • Trametes versicolor was more efficient in selective degradation of pine wood chips. • Both fungal strains significantly decreased crystallinity index of biomass only after week 7 of degradation. • Structural analysis showed that Trametes versicolor and Stereum hirsutum increased porous surface area of woody biomass. - Abstract: Stereum hirsutum and Trametes versicolor, were studied over a period of 3–7 weeks for pretreatment of radiata pine wood chips. Chemical analysis of pretreated biomass showed that the two studied strains were able to selectively degrade lignin. Selective lignin degradation was greater in week 3 of the pretreatment by Trametes versicolor compared to the other strain. Lengthening pretreatment time increased both lignin and cellulose losses which caused a reduction in selective lignin degradation for both strains. X-ray diffractometry showed that after seven weeks of pretreatment, the crystallinity of the woody biomass was decreased significantly. It decreased from 46% for untreated wood chips to 37% and 44% for Stereum hirsutum and Trametes versicolor treated biomass, respectively. The pretreatment with these two white rot fungi showed that 3-week pretreatment provided a cellulose rich biomass with the minimum cellulose loss compared to the other time of pretreatment.

  20. Injury of leg somatotopy of corticospinal tract at corona radiata by ventriculoperitoneal shunt: A case report.

    Science.gov (United States)

    Jang, Sung Ho; Kwon, Younghyeon

    2018-03-01

    A 45-year-old right-handed female patient suffered head trauma after being hit by a truck that ran into a house. The patient lost consciousness for 1 hour and experienced posttraumatic amnesia for 1 month after the accident. She underwent conservative management for a subdural hematoma in the left frontotemporal lobes and intracerebral hematoma in the left frontal lobe. The patient's Glasgow Coma Scale score was 11. She underwent a VP shunt operation, approached through the right posterior parietal area of the brain, at 4 months after onset. Approximately, 6 months after onset, she was admitted to the rehabilitation department of a university hospital. She presented with moderate weakness of the left leg: Medical Research Council scores: hip flexor; 3, knee extensor; 3+, ankle dorsiflexor; 3-. Brain magnetic resonance imaging revealed a leukomalactic lesion in the right posterior corona radiata along the shunt. On 6-month (2 months after the shunt operation) diffusion tensor tractography, the left CST showed partial injury in the posterior portion compared with the right CST. On 6-month transcranial magnetic stimulation study, the motor-evoked potential obtained at the left tibialis anterior muscle revealed lower amplitude than that on the right side. Injury of leg somatotopy of a CST was demonstrated in a patient with leg weakness following a VP shunt operation.

  1. Reduction in flatulence factors in mung beans (Vigna radiata) using low-dose gamma-irradiation

    International Nuclear Information System (INIS)

    Machaiah, J.P.; Pednekar, M.D.; Thomas, P.

    1999-01-01

    Mungbeans (Vigna radiata), control and gamma-irradiated at insect disinfestation dose levels (0.25 and 0.75 kGy) were germinated (0-6 Bays) and the qualitative and quantitative changes in soluble carbohydrates were studied in detail. The key flatulence-producing raffinose family oligosaccharides inmungbeans were degraded in the irradiated samples at the onset of the germination (0-2 days) compared to the control where it occurred much later (>4days). However, the reducing sugars, mainly glucose, fructose and galactose, which are metabolised easily, were enhanced in the irradiated samples. At low dose (0.25 kGy), irradiation had no effect on germination and sprout length, indicating that irradiated beans are suitable for use as sprouted beans. These observations clearly indicate that gamma-irradiation at insect disinfestation dose levels improved the digestibility and nutritional quality of mung beans by reducing the content of oligosaccharides responsible for intestinal gas production. (C) 1999 Society of Chemical Industry

  2. Increased antioxidant activity and polyphenol metabolites in methyl jasmonate treated mung bean (Vigna radiata sprouts

    Directory of Open Access Journals (Sweden)

    Li LI

    Full Text Available Abstract Mung bean sprouts are a popular health food both in China and worldwide. We determined the optimal concentration of exogenous methyl jasmonate (MeJA for the promotion of the sprouting in mung beans (Vigna radiata. The 1,1-diphenyl-2- picrylhydrazyl radical (DPPH scavenging test showed that MeJA application resulted in significantly improved antioxidant capacity in the sprouts 72 h later. Measurement of total polyphenols in MeJA-treated beans from 0 to 168 h, using Folin–Ciocalteu colorimetry, showed that the polyphenols changing was significantly correlated with antioxidant activity. The main polyphenols isovitexin, kaempferol-3-O-rutinoside, daidzein, genistein, isoquercitrin, p-coumaric acid, and caffeic acid were quantified using high-performance liquid chromatography (HPLC/QqQ MS and partial least squares discriminant analysis (PLS-DA. MeJA promoted the production of polyphenols, metabolites, and antioxidants in the sprouts; therefore, its use may allow sprouts to be prepared more quickly or increase their nutritional value.

  3. Regeneration of Rocky Mountain bristlecone pine (Pinus aristata) and limber pine (Pinus flexilis) three decades after stand-replacing fires

    Science.gov (United States)

    Jonathan D. Coop; Anna W. Schoettle

    2009-01-01

    Rocky Mountain bristlecone pine (Pinus aristata) and limber pine (Pinus flexilis) are important highelevation pines of the southern Rockies that are forecast to decline due to the recent spread of white pine blister rust (Cronartium ribicola) into this region. Proactive management strategies to promote the evolution of rust resistance and maintain ecosystem function...

  4. POTENSI ALELOPAT DAUN PINUS (Pinus spp. SEBAGAI BIOHERBISIDA PRA TUMBUH PADA GULMA KROKOT (Portulaca oleracea

    Directory of Open Access Journals (Sweden)

    Lutfy Ditya Cahyanti

    2015-06-01

    Full Text Available The aim of this experiment was to study the effectof pine leaf as allelophaty on purslane germination. Theexperiment were conducted at screen house Departmentof Biology, Faculty of Mathematics and Science, BrawijayaUniversity. The research is experimental design by nonfactorial Completely Randomized Blok Design, with threereplications, consisted of eleven levels. Purslane seeds sprout with control treatment, signifi cantly different from seedssprout ability in treatment solution leaves Pinus merkusii 2000ppm, and solution leaves of P. longaeva 2000 ppm. The resultshowed that 2000 ppm of P. merkusii extraction signifi cantlysuppressed 46% of purslane germination whereas 2000 ppmPinus longeava extraction signifi cantly suppressed of 41%campared to without any treatments (control.

  5. Novel ssDNA Viruses Detected in the Virome of Bleached, Habitat-Forming Kelp Ecklonia radiata

    Directory of Open Access Journals (Sweden)

    Douglas T. Beattie

    2018-01-01

    Full Text Available Kelp forests provide essential habitats for organisms in temperate rocky shores. Loss of kelp forests has occurred over large areas in a number of temperate regions, including in Australia, where the dominant kelp Ecklonia radiata has been lost from substantial areas of the shoreline. Loss of E. radiata has been associated with environmental stressors, including increased temperature and anthropogenic contaminants, as well as biological factors, such as herbivory. Disease may also play a role, but there is little information on the role of disease in the loss of kelp from coastal ecosystems or on the potential role of pathogenic microorganisms, such as viruses. E. radiata across much of its distribution in Australia can develop a “bleached” phenotype, which may be a disease. To investigate whether the phenotype was associated with a potential viral agent, we shotgun sequenced viral particles that were isolated from kelp with normal (healthy and bleached phenotypes. Each virome consisted of ~380,000 reads, of which ~25% were similar to known viruses. All samples were dominated by bacteriophages, but novel ssDNA virus sequences were detected that were almost exclusively in viromes from the bleached kelp phenotype. These ssDNA viruses are covered by 11 contigs that contained complete capsids and characteristic rep genes that were 30–60% similar to those of circular, Rep-encoding ssDNA viruses (CRESS-DNA viruses. CRESS-DNA viruses have not previously been described from macroalgae, and the rep genes were similar to CRESS-DNA viruses from marine water samples, snails, crabs, anemones, but also dragonflies. This raises the interesting possibility that the kelp could be a vector of the CRESS-DNA viruses to other organisms that are associated with the bleached state.

  6. Study of the effects of gamma sterilization on a bio material potential bone substitute: the nacre of Pinctada radiata (Leach, 1814)

    International Nuclear Information System (INIS)

    Ben Ammar, Rim

    2010-01-01

    In this work, we studied the effects of gamma irradiation at a dose of 25 kGy on physico-chemical properties of the nacre of the pearl oyster P. radiata Tunisian coast. The aim of our work is, after sterilizing the shell to gamma radiation, the study of its crystal structure, behavior and thermodynamics of the qualitative and quantitative composition of organic and inorganic matrices and compare them with those of the non-irradiated pearl . In addition, there has been interest in developing the method of sterilization with gamma irradiation to 25 kGy of the nacre of the pearl oyster Pinctada radiata.

  7. Genetic Analysis of Pinus sylvestris L. and Pinus sylvestris forma turfosa L. Using RAPD Markers

    Directory of Open Access Journals (Sweden)

    Beáta ÁBRAHÁM

    2010-03-01

    Full Text Available The purpose of the present study was to determine the level of genetic diversity within and among Ciuc basin, Romania (populations from Mohos and Luci raised bogs in Harghita Mountain and Sumuleu in Ciuc Mountain Pinus sylvestris populations using molecular markers. Two of populations (Mohos and Luci seems to be the descendants that survived the continental glaciation. Genetic diversity was analyzed by RAPD (Random Amplified Polymorphic DNA. Nine primers were selected for analysis, which generated reproducible bands. On base of presence or absence of homologues bands Nei’s gene diversity, the percentage of polymorphic loci and Nei’s unbiased genetic distance were calculated. The level of genetic variation among populations was found to be low. For both populations the variation values among populations were higher than within populations. The fossil records and geological historical data explain the extremely low genetic diversity of this species. Pinus sylvestris experienced strong bottlenecks during its evolutionary history, which caused the loss of genetic variation. Genetic drift and breeding in post-bottlenecked small populations may be the major forces that contribute to low genetic diversity and genetic differentiation of populations. Human activities may have accelerated the loss of genetic diversity in Pinus sylvestris.

  8. Density determination in Pino Radiata (D.Don) samples using 59.5 keV gamma radiation attenuation

    International Nuclear Information System (INIS)

    Dinator, Maria I.; Morales, Jose R.; Aliaga, Nelson; Karsulovic, Jose T.; Sanchez, Jaime; Leon, Adolfo

    1996-01-01

    A non destructive method to determine wood samples density is presented. The photon mass attenuation coefficient in samples of Pino radiata (D.Don) was measured at 59.5 keV with a radioactive source of Am-241. The value of 0.192 ± 0.002 cm 2 /g was obtained with a gamma spectroscopy system and later used on the determination of the mass density in sixteen samples of the same species. Comparison of these results with those of gravimetric method through a linear regression showed a slope of 1.001 and a correlation factor of 0.94. (author)

  9. Coronal MRI in the diagnosis of corona radiata infarcts adjacent to the lateral ventricles

    Energy Technology Data Exchange (ETDEWEB)

    Iwamoto, Toshihiko; Abe, Shin-e; Kanaya, Kiyoshi; Kubo, Hideki; Takasaki, Masaru (Tokyo Medical Coll. (Japan))

    1992-04-01

    To clarify pathophysiology of vascular lesions in corona radiata infarcts adjacent to the body of the lateral ventricle (CRILV), 17 patients with CRILV were studied by using magnetic resonance (MR) imaging. Based on the coronal MR images obtained between the anterior and posterior commissures, the patients were divided into two groups: (I) the group having infarcts below a line extending from the upper edge of the insular cistern to the external angle of the lateral ventricle adjacent to the corpus callosum (n=11); and (II) the group having lesions above the line towards the centrum semiovale (n=6). Most of the patients in both groups were male and had hypertension. Symptoms were gradually deteriorated and stroke in the morning was frequent in both groups, althrough the time from onset to completion was longer in Group II than Group I. In comparing clinical manifestations in Groups I and II, pure motor hemiparesis was found in 5 and 3 patients, and sensorimotor stroke in 2 and one patients, respectively. Drowsiness was found in 2 for Group I, while aphasia occurred in 2 for Group II. In addition, pseudobular and asymptomatic palsy occurred in each one patient for Group I. Prognosis was favorable in both groups, except for 2 who died of aspiration pneumonia. In Group I, 11 infarcts were well-defined, less than 15 mm in diameter on CT scans and were sclerotic in the main arteries without obstruction on angiograms. In contrast, Group II had ill-defined, larger low-density areas on CT scans and trunk obstruction of the internal carotid or middle cerebral arteries on angiograms. SPECT scans showed diffuse defect in Group II, in contrast to normal to diffuse or multiple defects in Group I. In Group I, lacunar lesions seemed to be caused by ischemia of the perforators, and in Group II, terminal zone infarcts in the territory of the cortical branches or watershed infarcts seemd to be caused by main trunk obstruction. (N.K.).

  10. Genetic variation for phytic acid content in mungbean (Vigna radiata L. Wilczek

    Directory of Open Access Journals (Sweden)

    Vinod Janardan Dhole

    2015-03-01

    Full Text Available Mungbean (Vigna radiata L. Wilczek is a short-duration legume crop cultivated for seeds that are rich in protein and carbohydrates. Mungbeans contain phytic acid (PA, an anti-nutritional factor that is the main storage form of organic phosphorus in seeds. It is a strong inhibitor against the absorption of nutrients including iron, zinc, calcium and magnesium in monogastric animals. Genotypes with low phytic acid (lpa in seed may show increased assimilation of nutrients and be useful in breeding lpa cultivars. The present study was conducted to identify lpa sources, genetic variation, heritability, and association with seed coat color, inorganic phosphorus (IP, and seed size in 102 mungbean genotypes including released varieties, land races, mutants, and wild species grown in two seasons: summer 2011 and rabi 2012. PA and IP in dry seeds were estimated by modified colorimetric method and Chen's modified method, respectively. PA, IP, and 100-seed weight differed significantly in the two seasons. PA content in 102 genotypes ranged from 5.74 to 18.98 mg g− 1 and 5.85 to 20.02 mg g− 1 in summer 2011 and rabi 2012, respectively. High heritability was found for PA (0.87 and 0.86 and seed size (0.82 and 0.83 but low heritability for IP (0.61 and 0.60. A negative correlation was found between PA and seed size (r = − 0.183 and − 0.267. Yellow and green seed coat genotypes contained significantly less PA than black seed coat genotypes. Cluster analysis revealed the distinctness of wild species, land races and cultivated varieties on the basis of PA content. The genotypes YBSM (6.001 mg g− 1 and JL-781 (6.179 mg g− 1 showed lowest PA. These lpa sources can be used to develop high-yielding mungbean cultivars with low phytic acid.

  11. Mobile phone radiation inhibits Vigna radiata (mung bean) root growth by inducing oxidative stress

    Energy Technology Data Exchange (ETDEWEB)

    Sharma, Ved Parkash [Department of Environment and Vocational Studies, Panjab University, Chandigarh 160014 (India); Department of Zoology, Panjab University, Chandigarh 160014 (India); Singh, Harminder Pal, E-mail: hpsingh_01@yahoo.com [Department of Environment and Vocational Studies, Panjab University, Chandigarh 160014 (India); Kohli, Ravinder Kumar; Batish, Daizy Rani [Department of Botany, Panjab University, Chandigarh 160014 (India)

    2009-10-15

    During the last couple of decades, there has been a tremendous increase in the use of cell phones. It has significantly added to the rapidly increasing EMF smog, an unprecedented type of pollution consisting of radiation in the environment, thereby prompting the scientists to study the effects on humans. However, not many studies have been conducted to explore the effects of cell phone EMFr on growth and biochemical changes in plants. We investigated whether EMFr from cell phones inhibit growth of Vigna radiata (mung bean) through induction of conventional stress responses. Effects of cell phone EMFr (power density: 8.55 {mu}W cm{sup -2}; 900 MHz band width; for 1/2, 1, 2, and 4 h) were determined by measuring the generation of reactive oxygen species (ROS) in terms of malondialdehyde and hydrogen peroxide (H{sub 2}O{sub 2}) content, root oxidizability and changes in levels of antioxidant enzymes. Our results showed that cell phone EMFr significantly inhibited the germination (at {>=}2 h), and radicle and plumule growths ({>=}1 h) in mung bean in a time-dependent manner. Further, cell phone EMFr enhanced MDA content (indicating lipid peroxidation), and increased H{sub 2}O{sub 2} accumulation and root oxidizability in mung bean roots, thereby inducing oxidative stress and cellular damage. In response to EMFr, there was a significant upregulation in the activities of scavenging enzymes, such as superoxide dismutases, ascorbate peroxidases, guaiacol peroxidases, catalases and glutathione reductases, in mung bean roots. The study concluded that cell phone EMFr inhibit root growth of mung bean by inducing ROS-generated oxidative stress despite increased activities of antioxidant enzymes.

  12. Mobile phone radiation inhibits Vigna radiata (mung bean) root growth by inducing oxidative stress

    International Nuclear Information System (INIS)

    Sharma, Ved Parkash; Singh, Harminder Pal; Kohli, Ravinder Kumar; Batish, Daizy Rani

    2009-01-01

    During the last couple of decades, there has been a tremendous increase in the use of cell phones. It has significantly added to the rapidly increasing EMF smog, an unprecedented type of pollution consisting of radiation in the environment, thereby prompting the scientists to study the effects on humans. However, not many studies have been conducted to explore the effects of cell phone EMFr on growth and biochemical changes in plants. We investigated whether EMFr from cell phones inhibit growth of Vigna radiata (mung bean) through induction of conventional stress responses. Effects of cell phone EMFr (power density: 8.55 μW cm -2 ; 900 MHz band width; for 1/2, 1, 2, and 4 h) were determined by measuring the generation of reactive oxygen species (ROS) in terms of malondialdehyde and hydrogen peroxide (H 2 O 2 ) content, root oxidizability and changes in levels of antioxidant enzymes. Our results showed that cell phone EMFr significantly inhibited the germination (at ≥2 h), and radicle and plumule growths (≥1 h) in mung bean in a time-dependent manner. Further, cell phone EMFr enhanced MDA content (indicating lipid peroxidation), and increased H 2 O 2 accumulation and root oxidizability in mung bean roots, thereby inducing oxidative stress and cellular damage. In response to EMFr, there was a significant upregulation in the activities of scavenging enzymes, such as superoxide dismutases, ascorbate peroxidases, guaiacol peroxidases, catalases and glutathione reductases, in mung bean roots. The study concluded that cell phone EMFr inhibit root growth of mung bean by inducing ROS-generated oxidative stress despite increased activities of antioxidant enzymes.

  13. Phosphorus Response and Amino Acid Composition of Different Green Gram (Vigna radiata L. Genotypes from Myanmar

    Directory of Open Access Journals (Sweden)

    M. Kywe

    2007-10-01

    Full Text Available Mungbean or green gram (Vigna radiata L. is an important component of rice-based cropping systems in Myanmar, where grain yields of around 800 kg ha^(-1 are much below its yield potential of 3000 kg ha^(-1. The reasons for this shortfall are as under-investigated as is the genotype-specific response of this crop to phosphorus (P application, which is critically low in many Myanmar soils, and the genetic variation in grain quality. For green gram quality, the concentration of lysine, an essential amino acid is particularly important given its scarcity in many cereal-based diets of Southeast Asia. The purpose of this study therefore was to investigate the effects of P application on the root and shoot growth, yield and its components for a range of green gram varieties, and to analyse the protein concentration and amino acid composition in green gram seed of different origins. To this end from 2001 to 2003, field experiments were conducted under rain-fed conditions in Yezin and Nyaung Oo. Fifteen landraces and five introduced green gram cultivars were grown at two levels of P (0 and 15 kg ha^(-1. There were large genotypic differences in P effects and a significant interaction between green gram genotypes and P for shoot and root growth. An unexpected benefit of P application was a reduction of pest and plant virus infestation in the field. Significant genotypic differences in the amino acid profile of seeds were also observed. The results indicate the potential for breeding efforts to increase seed yield and protein quality in green gram.

  14. Influence of distillery effluent on germination and growth of mung bean (Vigna radiata) seeds

    Energy Technology Data Exchange (ETDEWEB)

    Kannan, A. [Biomembrane Toxicology Division, Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, Lucknow 226001 (India); Upreti, Raj K. [Biomembrane Toxicology Division, Industrial Toxicology Research Centre, Post Box No. 80, M.G. Marg, Lucknow 226001 (India)], E-mail: upretirk@rediffmail.com

    2008-05-01

    Distillery effluent or spent wash discharged as waste water contains various toxic chemicals that can contaminate water and soil and may affect the common crops if used for agricultural irrigation. Toxic nature of distillery effluent is due to the presence of high amounts of organic and inorganic chemical loads and its high-acidic pH. Experimental effects of untreated (Raw) distillery effluent, discharged from a distillery unit (based on fermentation of alcohol from sugarcane molasses), and the post-treatment effluent from the outlet of conventional anaerobic treatment plant (Treated effluent) of the distillery unit were studied in mung bean (Vigna radiata, L.R. Wilczek). Mung bean is a commonly used legume crop in India and its neighboring countries. Mung bean seeds were presoaked for 6 h and 30 h, respectively, in different concentrations (5-20%, v/v) of each effluent and germination, growth characters, and seedling membrane enzymes and constituents were investigated. Results revealed that the leaching of carbohydrates and proteins (solute efflux) were much higher in case of untreated effluent and were also dependent to the presoaking time. Other germination characters including percentage of germination, speed of germination index, vigor index and length of root and embryonic axis revealed significant concentration-dependent decline in untreated effluent. Evaluation of seedlings membrane transport enzymes and structural constituents (hexose, sialic acid and phospholipids) following 6 h presoaking of seeds revealed concentration-dependent decline, which were much less in treated effluent as compared to the untreated effluent. Treated effluent up to 10% (v/v) concentration reflected low-observed adverse effect levels.

  15. Volatile constituents of Pinus roxburghii from Nepal.

    Science.gov (United States)

    Satyal, Prabodh; Paudel, Prajwal; Raut, Josna; Deo, Akash; Dosoky, Noura S; Setzer, William N

    2013-01-01

    Pinus roxburghii Sarg. Is one of 3 species of pine found in Nepal, the oil of which is traditionally used to treat cuts, wounds, boils, and blisters. To obtain, analyze, and examine the anti-microbial and cytotoxic activities of the essential oils of P. roxburghii. Three plant parts (cone, needle, and bark) of Pinus roxburghii were collected in Biratnagar, Nepal. The essential oils were obtained by hydrodistillation, and the chemical compositions were determined by GC-MS. The needle and cone essential oils were screened for anti-microbial activity against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Aspergillus niger; brine shrimp (Artemia salina) lethality; and in-vitro cytotoxicity against MCF-7 cells. GC-MS analysis for the cone oil revealed 81 compounds with 78 components being identified (95.5% of the oil) while 98.3% of needle oil was identified to contain 68 components and 98.6% of the bark oil (38 components) was identified. The 3 essential oils were dominated by sesquiterpenes, particularly (E)-caryophyllene (26.8%-34.5%) and α-humulene (5.0%-7.3%) as well as monoterpene alcohols terpinen-4-ol (4.1%-30.1%) and α-terpineol(2.8%-5.0%). The monoterpene δ-3-carene was present only in needle and cone essential oils (2.3% and 6.8%, respectively). Bio-activity assays of the cone essential oil of P. roxburghii showed remarkable cytotoxic activity (100% killing of MCF-7 cells at 100 μg/mL) along with notable brine shrimp lethality (LC50 =11.8 μg/mL). The cone essential oil did not show anti-bacterial activity, but it did exhibit anti-fungal activity against Aspergillus niger (MIC=39 μg/mL). The bioactivity of P. roxburghii essential oil is consistent with its traditional medicinal use.

  16. Phytochemical analysis of Pinus eldarica bark

    Science.gov (United States)

    Iravani, S.; Zolfaghari, B.

    2014-01-01

    Bark extract of Pinus pinaster contains numerous phenolic compounds such as catechins, taxifolin, and phenolic acids. These compounds have received considerable attentions because of their anti-inflammatory, antimutagenic, anticarcinogenic, antimetastatic and high antioxidant activities. Although P. pinaster bark has been intensely investigated in the past; there is comparably less information available in the literature in regard to P. eldarica bark. Therefore, the aim of this study was to determine the chemical composition of P. eldarica commonly found in Iran. A reversed-phase high pressure liquid chromatography (RP-HPLC) method for the determination of catechin, caffeic acid, ferulic acid, and taxifolin in P. pinaster and P. eldarica was developed. A mixture of 0.1% formic acid in deionized water and 0.1% formic acid in acetonitrile was used as the mobile phase, and chromatographic separation was achieved on a Nova pack C18 at 280 nm. The two studied Pinus species contained high amounts of polyphenolic compounds. Among four marker compounds, the main substances identified in P. pinaster and P. eldarica were taxifolin and catechin, respectively. Furthermore, the composition of the bark oil of P. eldarica obtained by hydrodistillation was analyzed by gas chromatography/mass spectroscopy (GC/MS). Thirty-three compounds accounting for 95.1 % of the oil were identified. The oils consisted mainly of mono- and sesquiterpenoid fractions, especially α-pinene (24.6%), caryophyllene oxide (14.0%), δ-3-carene (10.7%), (E)-β-caryophyllene (7.9%), and myrtenal (3.1%). PMID:25657795

  17. Fire effects in Pinus uncinata Ram plantations

    Directory of Open Access Journals (Sweden)

    Adrián Cardil Forradellas

    2016-04-01

    Full Text Available Aim of study: Understanding fire ecology of main forest species is essential for a sound, scientifically based on managing of wildlands and also to assess likely implications due to changes in fire regime under a global change scenario. Few references can be found about fire ecology of Pinus uncinata Ram. (PU. PU species grows in the Central Pyrenees where large, severe wildland fires did not occur frequently in the past. However, several fires with extreme fire behavior have affected PU stands in last years and they might disturb other PU forest in the future.Area of study: Cabdella fire (February 2012, in Lleida province, is one of the several wildland fires occurred in 2012 (winter season in the Central Pyrenees. Fire affected a large PU plantation (102 ha located at 1.800-2,100 meters above the sea.Material and methods: We have analyzed first order fire effects in three fireline intensity thresholds along three years in terms of mortality ratio, scorched height, percentage of scorched crown volume and bark char height.Main results: PU seems to be a very tolerant species to low and medium fire line intensity but fire effects were very significant when fire line intensity was high. In medium fireline intensity sites, probability of mortality ranged from 15 to 30% and the dead trees had the highest values on scorched height and percentage of scorched crown volume.Research highlights: Results from this work supports that prescribed burning might be used to efficiently decrease fuel load and fuel vertical continuity while avoiding considerable PU mortality. It also displayed that when fuel management has been implemented, PU mortality might be limited even under extreme fire behavior.Abbreviations used: PU: Pinus uncinata Ram.

  18. Analisis Komponen Kimia Dan Uji Aktivitas Antibakteri Minyak Atsiri Daun Pinus (Pinus Merkusii Jungh.Et Devries) Dari Kabupaten Samosir

    OpenAIRE

    Siringo-Ringo, Mawar

    2015-01-01

    Essential oil of pinus leaves (Pinus merkusii Jungh.et deVries) have been isolated by hydrodestilation method using Stahl. Pinus leaves have destilated for five hours roduced essential oil 0.1531% (w/w). The results of the analyse use GC-MS showed 23 peaks and can be identified 20 compounds and have five major compounds are Limonene (22.72%), α-Pinene (17.53%), β-Caryophyllene (16.76%), β-Ocimene (14.68%), and Germacren-d (11.24%). Antibacterial activity of the test have been done using ag...

  19. Efficacy of insecticides against army worm (spodoptera mauritia) on mung bean (vigna radiata l.) under arid climate

    International Nuclear Information System (INIS)

    Abbas, G.; Aslam, M.; Khokhar, M.B.; Khattak, J.Z.K.; Malik, A.U.

    2011-01-01

    Influence of Bifenthrine (Talstar) at the rate 375 ml ha/sup -1/, Deltaphos 10+350 EC at the rate 500 ml ha/sup -1/, Lorsban 40 EC at the rate 850 ml ha/sup -1/, Triazofos (20/400 EC) at the rate 750 ml ha/sup -1/and Karate 5 EC at the rate 1250 ml ha/sup -1/ was studied on mung bean (Vigna radiata L.) yield under arid climate at Adaptive Research Farm, Karor during two kharif seasons of 2007 and 2008. Experiments were laid out in randomized complete block design with six a test variety. All the chemicals showed significant impact on mung crop as compared to that in control treatments. AZRI- 2006, a promising variety of mung bean (Vigna radiata L.) for arid climate was used as plots, but the treatment of Deltaphos 10+350 EC at the rate 350 ml ha/sup -1/consistently proved better than other treatments. (author)

  20. Diffusion tensor magnetic resonance imaging for hand and foot fibers location at the corona radiata: comparison with two lesion studies

    Directory of Open Access Journals (Sweden)

    Dong-Hoon eLee

    2014-09-01

    Full Text Available The corticospinal tract is the motor pathway in the human brain, and corona radiata is an important location to diagnose stroke. We detected hand and foot motor fiber tracts in the corona radiata to investigate accurate locations using diffusion tensor imaging and functional imaging. Ten right-handed normal volunteers participated in this study. We used a probabilistic tracking algorithm, a brain normalization method, and functional imaging results to set out ROIs. Moreover, our results were compared to previous results of lesion studies to confirm their accuracy and usefulness. The location measurements were performed in two index types; anteriority index on the basis of the anterior and posterior location of lateral ventricle, laterality index on the basis of the left and right location. The anteriority indices were 56.40/43.2 (hand/foot at the upper CR and lower CR 40.72/30.90 at the lower CR. The measurements of anteriority and laterality of motor fibers were represented as anteriority index 0.40/0.31 and laterality index 0.60/0.47 (hand/foot. Our results showed that the hand and foot fibers were in good agreements with previous lesion studies. This study and approaches can be used as a standard for diffusion tensor image combined with lesion location studies in patients who need rehabilitation or follow up.

  1. In silico genome-wide identification and characterization of the glutathione S-transferase gene family in Vigna radiata.

    Science.gov (United States)

    Vaish, Swati; Awasthi, Praveen; Tiwari, Siddharth; Tiwari, Shailesh Kumar; Gupta, Divya; Basantani, Mahesh Kumar

    2018-05-01

    Plant glutathione S-transferases (GSTs) are integral to normal plant metabolism and biotic and abiotic stress tolerance. The GST gene family has been characterized in diverse plant species using molecular biology and bioinformatics approaches. In the current study, in silico analysis identified 44 GSTs in Vigna radiata. Of the total 44 GSTs identified, chromosomal locations of 31 GSTs were confirmed. The pI value of GST proteins ranged from 5.10 to 9.40. The predicted molecular weights ranged from 13.12 to 50 kDa. Subcellular localization analysis revealed that all GSTs were predominantly localized in the cytoplasm. The active site amino acids were confirmed to be serine in tau, phi, theta, zeta, and TCHQD; cysteine in lambda, DHAR, and omega; and tyrosine in EF1G. The gene architecture conformed to the two-exon/one-intron and three-exon/two-intron organization in the case of tau and phi classes, respectively. MEME analysis identified 10 significantly conserved motifs with the width of 8-50 amino acids. The motifs identified were either specific to a specific GST class or were shared by multiple GST classes. The results of the current study will be of potential importance in the characterization of the GST gene family in V. radiata, an economically important leguminous crop.

  2. Ionizing radiation induced changes in phenotype, photosynthetic pigments and free polyamine levels in Vigna radiata (L.) Wilczek

    International Nuclear Information System (INIS)

    Sengupta, Mandar; Chakraborty, Anindita; Raychaudhuri, Sarmistha Sen

    2013-01-01

    Effects of gamma rays on the free polyamine (PA) levels were studied in Vigna radiata (L.) Wilczek. Seeds exposed to different doses of gamma rays were checked for damage on phenotype, germination frequency and alteration in photosynthetic pigments. Free polyamine levels were estimated from seeds irradiated in dry and water imbibed conditions. Polyamine levels of seedlings grown from irradiated seeds, and irradiated seedlings from unexposed seeds were also measured. Damage caused by gamma irradiation resulted in decrease in final germination percentage and seedling height. Photosynthetic pigments decreased in a dose dependent manner as marker of stress. Polyamines decreased in irradiated dry seeds and in seedlings grown from irradiated seeds. Radiation stress induced increase in free polyamines was seen in irradiated imbibed seeds and irradiated seedlings. Response of polyamines towards gamma rays is dependent on the stage of the life cycle of the plant. - Highlights: ► Gamma irradiation of Vigna radiata (L.) Wilczek seeds and seedlings. ► Decrease in germination frequency. ► Increase in seedling injury with increased dosage of gamma rays. ► Decrease in chlorophyll and carotenoid pigments. ► Change in free polyamine levels

  3. Nontarget effects of chemical pesticides and biological pesticide on rhizospheric microbial community structure and function in Vigna radiata.

    Science.gov (United States)

    Singh, Sunil; Gupta, Rashi; Kumari, Madhu; Sharma, Shilpi

    2015-08-01

    Intensive agriculture has resulted in an indiscriminate use of pesticides, which demands in-depth analysis of their impact on indigenous rhizospheric microbial community structure and function. Hence, the objective of the present work was to study the impact of two chemical pesticides (chlorpyrifos and cypermethrin) and one biological pesticide (azadirachtin) at two dosages on the microbial community structure using cultivation-dependent approach and on rhizospheric bacterial communities involved in nitrogen cycle in Vigna radiata rhizosphere through cultivation-independent technique of real-time PCR. Cultivation-dependent study highlighted the adverse effects of both chemical pesticide and biopesticide on rhizospheric bacterial and fungal communities at different plant growth stages. Also, an adverse effect on number of genes and transcripts of nifH (nitrogen fixation); amoA (nitrification); and narG, nirK, and nirS (denitrification) was observed. The results from the present study highlighted two points, firstly that nontarget effects of pesticides are significantly detrimental to soil microflora, and despite being of biological origin, azadirachtin exerted negative impact on rhizospheric microbial community of V. radiata behaving similar to chemical pesticides. Hence, such nontarget effects of chemical pesticide and biopesticide in plants' rhizosphere, which bring out the larger picture in terms of their ecotoxicological effect, demand a proper risk assessment before application of pesticides as agricultural amendments.

  4. Late Eocene white pines (Pinus subgenus Strobus) from southern China.

    Science.gov (United States)

    Xu, Qingqing; Zhou, Wenjun; Kodrul, Tatiana M; Naugolnykh, Serge V; Jin, Jianhua

    2015-11-09

    Fossil records indicate that the genus Pinus L. split into two subgenera by the Late Cretaceous, although subgenus Strobus (D. Don) Lemmon is less well documented than subgenus Pinus L., especially in eastern Asia. In this paper, Pinus maomingensis sp. nov. is established based on a compressed seed cone from the upper Eocene of the Maoming Basin of southern China. This species is attributed to genus Pinus, subgenus Strobus, section Quinquefoliae Duhamel, subsection Strobus Loudon based on the combination of morphological characters obtained from the cone scales, specifically from the terminal umbo, rhombic apophysis, and cuticle structure. Associated fascicles of needle leaves with deciduous sheaths and bulbous bases are recognized as Pinus sp. and also represent Pinus subgenus Strobus. This new discovery from the Maoming Basin constitutes the first megafossil record of subgenus Strobus from southern China and implies that the members of this subgenus arrived in the southern region of China by the late Eocene. The extant species of subgenus Strobus are mainly distributed in northern temperate and tropical to subtropical mountainous regions. We propose that the Maoming Basin was adjacent to a mountainous region during the late Eocene.

  5. Ocean acidification reverses the positive effects of seawater pH fluctuations on growth and photosynthesis of the habitat-forming kelp, Ecklonia radiata.

    Science.gov (United States)

    Britton, Damon; Cornwall, Christopher E; Revill, Andrew T; Hurd, Catriona L; Johnson, Craig R

    2016-05-27

    Ocean acidification (OA) is the reduction in seawater pH due to the absorption of human-released CO2 by the world's oceans. The average surface oceanic pH is predicted to decline by 0.4 units by 2100. However, kelp metabolically modifies seawater pH via photosynthesis and respiration in some temperate coastal systems, resulting in daily pH fluctuations of up to ±0.45 units. It is unknown how these fluctuations in pH influence the growth and physiology of the kelp, or how this might change with OA. In laboratory experiments that mimicked the most extreme pH fluctuations measured within beds of the canopy-forming kelp Ecklonia radiata in Tasmania, the growth and photosynthetic rates of juvenile E. radiata were greater under fluctuating pH (8.4 in the day, 7.8 at night) than in static pH treatments (8.4, 8.1, 7.8). However, pH fluctuations had no effect on growth rates and a negative effect on photosynthesis when the mean pH of each treatment was reduced by 0.3 units. Currently, pH fluctuations have a positive effect on E. radiata but this effect could be reversed in the future under OA, which is likely to impact the future ecological dynamics and productivity of habitats dominated by E. radiata.

  6. Analysis of disconnected diallel mating designs II: results from a third generation progeny test of the New Zealand radiata pine improvement programme.

    Science.gov (United States)

    J.N. King; M.J. Carson; G.R. Johnson

    1998-01-01

    Genetic parameters from a second generation (F2) disconnected diallel progeny test of the New Zealand radiata pine improvement programme are presented. Heritability estimates of growth and yield traits of 0.2 are similar to progeny test results of the previous generation (F1) generation tests. A trend of declining dominance...

  7. Acute Toxicity of Fresh and Aged Residues of Pesticides to the Parasitoid Tamarixia radiata and to the HLB-Bacteria Vector Diaphorina citri.

    Science.gov (United States)

    Beloti, V H; Alves, G R; Moral, R A; Demétrio, C G B; Yamamoto, P T

    2018-06-01

    One method for controlling the Asian citrus psyllid (ACP) Diaphorina citri Kuwayama, the vector of the putative causal agent of Huanglongbing, uses the parasitoid Tamarixia radiata (Waterston). However, the general intensive use of insecticides has reduced the numbers of this parasitoid. This study evaluated the effect of the residual action of 24 insecticides on T. radiata and also determined the differential toxicity of insecticides to D. citri and T. radiata, using three bioassays. In the first, when adults of the parasitoid were exposed to residues of the 24 insecticides, ten were considered short-life (class 1), six slightly persistent (class 2), five moderately persistent (class 3), and three insecticides were considered persistent (class 4), under the IOBC/WPRS classification system. The second bioassay evaluated the sublethal concentrations of the persistent insecticides (formetanate, dimethoate, spinosad). Increasing the concentrations of the insecticides increased the number that were classified as persistent. In the third bioassay, evaluation of the differential toxicity of eight insecticides to the ACP and the parasitoid showed that chlorpyrifos and bifenthrin were more harmful to T. radiata. Therefore, these two insecticides are not recommended for application at the time of parasitoid release. Cypermethrin, imidacloprid, and dimethoate caused higher mortality of D. citri and are most often recommended in IPM programs. The choice of an insecticide for the control of citrus pests must be made with care, aiming to preserve the natural enemies in the ecosystem, and thereby contribute to the success of biological control.

  8. Contrasting Effects of Aqueous Tissue Extracts from an Invasive Plant, Bidens pilosa L. var. radiata, on the Performance of Its Sympatric Plant Species

    Directory of Open Access Journals (Sweden)

    Hsiao-Mei Hsu

    2009-09-01

    Full Text Available Bidens pilosa L. var. radiata Sch. Bip., a common weed in lowland Taiwan, is listed as one of the twenty most noxious invasive plants in Taiwan. In this study, we examined the effect of aqueous extracts of leaves, stems and roots of the invasive plant on germination and growth of seedlings (estimated by measuring the elongation of hypocotyls and radicals of the same species and two other sympatric species, B. bipinnata and Ageraturem conyzoides. The objective of this study was to understand whether the aqueous tissue extracts affected the performance of the target species and whether these effects varied among tissue types and among target species. We found that the germination percentage of seeds of B. bipinnata was significantly reduced by root and leaf extracts, that of B. pilosa var. radiata was also significantly reduced by the application of root extract, while that of A. conyzoides was not affected by any of the three tissue extracts. The application of stem and leaf extracts inhibited the elongation of radicals of B. pilosa var. radiata, consequently, the growth of seedlings of this species was decreased in these two treatments. Though the elongation of hypocotyls was stimulated by leaf extract, the overall growth of seedlings of B. bipinnata was not affected by any tissue extract. In contrast, all three extracts stimulated the elongation of hypocotyls and radicals of A. conyzoides, consequently, the overall growth of seedlings of this plant was promoted by all three extracts. These results revealed that aqueous extracts from tissue of B. pilosa var. radiata had differential effect on the emergence and seedling growth of the three target species. The inhibition effect of its root and leaf extracts on the germination of B. bipinnata may partially explain the overwhelming dominance of B. pilosa var. radiata over B. bipinnata when they are sympatric.

  9. Dendrochronology of bristlecone pine, Pinus longaeva

    International Nuclear Information System (INIS)

    Ferguson, C.W.

    1979-01-01

    Since 1953 the Laboratory of Tree-Ring Research has conducted dendrochronological studies of bristlecone pine Pinus longaeva D.K. Bailey, sp. nov.) in the White Mountains of California. This research resulted in the establishment of a continuous tree-ring sequence of 8253 yr. The millennia-old pines have emerged as a unique source of chronological data and the precisely dated wood is essential to certain paleoenvironmental and geophysical investigations. Over 1000 dendrochronologically dated decade samples of bristlecone pine supplied to three C-14 laboratories have been used to calibrate the radiocarbon time scale for the past seven millennia, a development of far reaching consequences in the fields of archaeology and geology. In addition, recent advances in other methods of analyzing past climatic variability - techniques involving stable isotope ratios, amino acid racemization, remanent magnetism and trace element abundances - have greatly increased the demand for wood of known age and, hence, for chronology development. Spanning the past 7500 yr, 1138 prepared decade samples, with a total weight of nearly 16 kg are available for study. (author)

  10. Fusariose em Mudas de Pinus taeda Fusarium disease on Pinus taeda seedlings

    Directory of Open Access Journals (Sweden)

    Albino Grigoletti Júnior

    2011-03-01

    Full Text Available

    Viveiros comerciais têm apresentado mudas de Pinus taeda com sintomas de murcha e seca de ponteiros e morte, na Região Sul do Brasil. Isolamento em meio BDA e câmara úmida, teste de patogenicidade e microcultivo foram feitos para identificar o patógeno. Uma espécie de Fusarium foi isolada, cuja identificação encontra-se em andamento. Verificou-sepelos postulados de Koch que Fusarium sp. foi o agente causal dessa doença.
    Nurseries has presented Pinus taeda seedling with symptoms of wilt, tip blight and death, in Southern Region of Brazil. Isolation on PDA medium, moist chamber, pathogenicity test and microculture were made to identify the pathogen. A species of Fusarium was isolated, which is under identification. It was verified by Koch postulates that Fusarium sp. was the causal agent of this disease.

  11. Pinus nigra and Pinus pinaster needles as passive samplers of polycyclic aromatic hydrocarbons

    International Nuclear Information System (INIS)

    Piccardo, Maria Teresa; Pala, Mauro; Bonaccurso, Bruna; Stella, Anna; Redaelli, Anna; Paola, Gaudenzio; Valerio, Federico

    2005-01-01

    Nine polycyclic aromatic hydrocarbons (PAHs) were analysed in pine needles of different ages (from 6 to 30 months) collected from two species, Pinus nigra and Pinus pinaster, in seven sites located along a transect from a suburban to a rural area of Genoa (Italy). In all sites and for both species, concentrations of more volatile PAHs (phenanthrene, anthracene, fluoranthene, pyrene) were higher than those for other less volatile PAHs, which are preferentially sorbed to airborne particulates (benzo[a]anthracene, chrysene, benzofluoranthenes, benzo[a]pyrene). Concentrations of total PAHs found in P. nigra in the rural sites were, on the average, 2.3 times higher than those in P. pinaster growing nearby. In both pine species, concentrations of volatile PAHs increased according to needle age. Annual trends of other PAHs were more variable, with a general decrease in older needles. P. pinaster needles are shown to be more reliable passive samplers, since they are more resistant to plant diseases, and considerable variation in PAH concentration was observed in P. nigra needles with moulds and fungi. - The suitability of the pine needles as passive air samplers for persistent trace organics is demonstrated

  12. Pinus nigra and Pinus pinaster needles as passive samplers of polycyclic aromatic hydrocarbons

    Energy Technology Data Exchange (ETDEWEB)

    Piccardo, Maria Teresa [Environmental Chemistry Laboratory, National Cancer Research Institute, Genova. L.go Rosanna Benzi n. 10, 16132 Genova (Italy)]. E-mail: chimamb@istge.it; Pala, Mauro [Environmental Chemistry Laboratory, National Cancer Research Institute, Genova. L.go Rosanna Benzi n. 10, 16132 Genova (Italy); Bonaccurso, Bruna [Environmental Chemistry Laboratory, National Cancer Research Institute, Genova. L.go Rosanna Benzi n. 10, 16132 Genova (Italy); Stella, Anna [Environmental Chemistry Laboratory, National Cancer Research Institute, Genova. L.go Rosanna Benzi n. 10, 16132 Genova (Italy); Redaelli, Anna [Environmental Chemistry Laboratory, National Cancer Research Institute, Genova. L.go Rosanna Benzi n. 10, 16132 Genova (Italy); Paola, Gaudenzio [Botany Department, Genoa University, C.so Dogali 1 canc., 16136 Genova (Italy); Valerio, Federico [Environmental Chemistry Laboratory, National Cancer Research Institute, Genova. L.go Rosanna Benzi n. 10, 16132 Genova (Italy)

    2005-01-01

    Nine polycyclic aromatic hydrocarbons (PAHs) were analysed in pine needles of different ages (from 6 to 30 months) collected from two species, Pinus nigra and Pinus pinaster, in seven sites located along a transect from a suburban to a rural area of Genoa (Italy). In all sites and for both species, concentrations of more volatile PAHs (phenanthrene, anthracene, fluoranthene, pyrene) were higher than those for other less volatile PAHs, which are preferentially sorbed to airborne particulates (benzo[a]anthracene, chrysene, benzofluoranthenes, benzo[a]pyrene). Concentrations of total PAHs found in P. nigra in the rural sites were, on the average, 2.3 times higher than those in P. pinaster growing nearby. In both pine species, concentrations of volatile PAHs increased according to needle age. Annual trends of other PAHs were more variable, with a general decrease in older needles. P. pinaster needles are shown to be more reliable passive samplers, since they are more resistant to plant diseases, and considerable variation in PAH concentration was observed in P. nigra needles with moulds and fungi. - The suitability of the pine needles as passive air samplers for persistent trace organics is demonstrated.

  13. Evaluation of ischemic corticospinal tract damage by diffusion tensor MRI. Its significance to predict functional outcome of corona radiata infarct

    International Nuclear Information System (INIS)

    Tanaka, Hideki

    2010-01-01

    Motor impairment is one of the most frequent symptoms among stroke patients and often leads to poststroke dependency. Recent advances of diffusion tensor MR imaging made it possible to identify corticospinal tract (CST) three-dimensionally and evaluate structural damage, so precise evaluation of the ischemic CST damage became feasible.Motor impairment, lesion size and location upon diffusion weighted MR image and clinical outcome were assessed in 23 acute to subacute capsular and corona radiata infarct patients. According to the lesion size, patients were grouped into A, maximal diameter below 15 mm and B, that above 15 mm. Motor impairment was graded severe: limb movement synergy level, moderate: selective muscle activity possible and mild: isolated movements well co-ordinated, each corresponding to Brunnstrom stage 1-3, 4-5, and 6, respectively. Outcome at the time of discharge was assessed by modified Rankin Scale (mRS), discharge destination and length of hospital stay were also registered. Diffusion tensor MR imaging was conducted in 15 corona radiata infarct patients at 2.3+-2.2 days from the onset of the clinical symptoms. CST was 3-dimensionally identified with dTV. II. SR and Volume-one 1.72 and CST-FA ratio (ipsi-/contralesional CST-FA) and CST-Area% (CST lesion free area/whole CST area) were obtained at the level where ischemic damage was most prominent and correlation of these parameters to motor impairment and clinical outcome was studied. CST-FA ratio and CST-Area% were in good correlation to motor impairment at presentation. Patients with severe motor impairment had lower CST-FA ratio and CSF-Area% than those with moderate or mild. CST-FA ratio was 0.73+-0.22 in patients with poor clinical outcome (mRS 3-6) and 0.93+-0.09 with good clinical outcome (mRS 0-2) (p=0.038). Diffusion tensor MR imaging is useful in evaluating motor impairment and predicting functional outcome of corona radiata infarct patient in the acute to subacute stage. (author)

  14. Salt and drought stress and ABA responses related to bZIP genes from V. radiata and V. angularis.

    Science.gov (United States)

    Wang, Lanfen; Zhu, Jifeng; Li, Xiaoming; Wang, Shumin; Wu, Jing

    2018-04-20

    Mung bean and adzuki bean are warm-season legumes widely cultivated in China. However, bean production in major producing regions is limited by biotic and abiotic stress, such as drought and salt stress. Basic leucine zipper (bZIP) genes play key roles in responses to various biotic and abiotic stresses. However, only several bZIP genes involved in drought and salt stress in legumes, especially Vigna radiata and Vigna angularis, have been identified. In this study, we identified 54 and 50 bZIP proteins from whole-genome sequences of V. radiata and V. angularis, respectively. First, we comprehensively surveyed the characteristics of all bZIP genes, including their gene structure, chromosome distribution and motif composition. Phylogenetic trees showed that VrbZIP and VabZIP proteins were divided into ten clades comprising nine known and one unknown subgroup. The results of the nucleotide substitution rate of the orthologous gene pairs showed that bZIP proteins have undergone strong purifying selection: V. radiata and V. angularis diverged 1.25 million years ago (mya) to 9.20 mya (average of 4.95 mya). We also found that many cis-acting regulatory elements (CAREs) involved in abiotic stress and plant hormone responses were detected in the putative promoter regions of the bZIP genes. Finally, using the quantitative real-time PCR (qRT-PCR) method, we performed expression profiling of the bZIP genes in response to drought, salt and abscisic acid (ABA). We identified several bZIP genes that may be involved in drought and salt responses. Generally, our results provided useful and rich resources of VrbZIP and VabZIP genes for the functional characterization and understanding of bZIP transcription factors (TFs) in warm-season legumes. In addition, our results revealed important and interesting data - a subset of VrbZIP and VabZIP gene expression profiles in response to drought, salt and ABA stress. These results provide gene expression evidence for the selection of

  15. Effect of (/sup 60/cobalt) gamma rays on growth and root rot diseases in mungbean (vigna radiata L.)

    International Nuclear Information System (INIS)

    Ikram, N.; Dawar, S.; Zaki, M.J.; Abass, Z.

    2010-01-01

    Present investigation showed that gamma rays influences suppressive effect on root rot fungi such as Macrophomina phaseolina (Tassi) Goid, Rhizoctonia solani Kuhn and Fusarium spp., and inducive effect on growth parameters of mung bean (Vigna radiata L.). Seeds of mung bean were treated with gamma rays (/sup 60/Cobalt) at time periods of 0 and 4 minutes and stored for 90 days at room temperature to determine its effect on growth parameters and infection of root infecting fungi. All treatments of gamma rays enhanced the growth parameters as compared to untreated plants. Infection of M. phaseolina, R. solani and Fusarium spp., were significantly decreased on mung bean seeds treated with gamma rays. Gamma rays significantly increased the growth parameters and controlled the root rot fungi up to 90 days of storage of seeds. (author)

  16. Biofertilizing efficiency of Sargassum polycystum extract on growth and biochemical composition of Vigna radiata and Vigna mungo

    Directory of Open Access Journals (Sweden)

    B Bharath

    2018-01-01

    Full Text Available Objective: To evaluate the effect of marine brown alga Sargassum polycystum extract on growth and biochemical parameters of Vigna radiata and Vigna mungo.Methods: Different concentrations of algal extracts (0.5%, 1.0%, 2.0%, 3.0%, 4.0%, and 5.0% were prepared and applied to the crops at every 10-day intervals under natural conditions. After 30 d, the plants were harvested to evaluate the growth and biochemical parameters.Results: Seaweed liquid fertilizers treated seedlings showed maximum growth in 3.0% concentration when compared to the untreated seedlings. Similarly, biochemical parameters such as photosynthetic pigments, protein, reducing sugar, total sugar and amino acids exhibited increases in 3.0% concentration seaweed extract. Decreases in growth and biochemical parameters were noticed in concentrations higher than 3.0%.Conclusions: Presence of micronutrients and growth regulating substances in the liquid extract help healthier and faster productivity of the crop.

  17. Fire effects in Pinus uncinata Ram plantations

    Energy Technology Data Exchange (ETDEWEB)

    Cardil Forradellas, A.; Molina Terrén, D.M.; Oliveres, J.; Castellnou, M.

    2016-07-01

    Aim of study: Understanding fire ecology of main forest species is essential for a sound, scientifically based on managing of wildlands and also to assess likely implications due to changes in fire regime under a global change scenario. Few references can be found about fire ecology of Pinus uncinata Ram. (PU). PU species grows in the Central Pyrenees where large, severe wildland fires did not occur frequently in the past. However, several fires with extreme fire behavior have affected PU stands in last years and they might disturb other PU forest in the future. Area of study: Cabdella fire (February 2012), in Lleida province, is one of the several wildland fires occurred in 2012 (winter season) in the Central Pyrenees. Fire affected a large PU plantation (102 ha) located at 1.800-2,100 meters above the sea. Material and methods: We have analyzed first order fire effects in three fireline intensity thresholds along three years in terms of mortality ratio, scorched height, percentage of scorched crown volume and bark char height. Main results: PU seems to be a very tolerant species to low and medium fire line intensity but fire effects were very significant when fire line intensity was high. In medium fireline intensity sites, probability of mortality ranged from 15 to 30% and the dead trees had the highest values on scorched height and percentage of scorched crown volume. Research highlights: Results from this work supports that prescribed burning might be used to efficiently decrease fuel load and fuel vertical continuity while avoiding considerable PU mortality. It also displayed that when fuel management has been implemented, PU mortality might be limited even under extreme fire behavior. (Author)

  18. Relationship between diffusion tensor fractional anisotropy and motor outcome in patients with hemiparesis after corona radiata infarct.

    Science.gov (United States)

    Koyama, Tetsuo; Marumoto, Kohei; Miyake, Hiroji; Domen, Kazuhisa

    2013-11-01

    This study examined the relationship between fractional anisotropy (FA) values of magnetic resonance-diffusion tensor imaging (DTI) and motor outcome (1 month after onset) in 15 patients with hemiparesis after ischemic stroke of corona radiata lesions. DTI data were obtained on days 14-18. FA values within the cerebral peduncle were analyzed using a computer-automated method. Motor outcome of hemiparesis was evaluated according to Brunnstrom stage (BRS; 6-point scale: severe to normal) for separate shoulder/elbow/forearm, wrist/hand, and lower extremity functions. The ratio of FA values in the affected hemisphere to those in the unaffected hemisphere (rFA) was assessed in relation to the BRS data (Spearman rank correlation test, P<.05). rFA values ranged from .715 to 1.002 (median=.924). BRS ranged from 1 to 6 (median=4) for shoulder/elbow/forearm, from 1 to 6 (median=5) for wrist/hand, and from 2 to 6 (median=4) for the lower extremities. Analysis revealed statistically significant relationships between rFA and upper extremity functions (correlation coefficient=.679 for shoulder/elbow/forearm and .706 for wrist/hand). Although slightly less evident, the relationship between rFA and lower extremity function was also statistically significant (correlation coefficient=.641). FA values within the cerebral peduncle are moderately associated with the outcome of both upper and lower extremity functions, suggesting that DTI may be applicable for outcome prediction in stroke patients with corona radiata infarct. Copyright © 2013 National Stroke Association. Published by Elsevier Inc. All rights reserved.

  19. Halopriming of seeds imparts tolerance to NaCl and PEG induced stress in Vigna radiata (L.) Wilczek varieties.

    Science.gov (United States)

    Jisha, K C; Puthur, Jos T

    2014-07-01

    The investigation was carried out to study the effect of halopriming on NaCl and polyethylene glycol-6000 (PEG-6000) induced stress tolerance potential of three Vigna radiata (L.) Wilczek varieties, with varied abiotic stress tolerance potential. Halopriming is a seed priming technique in which the seeds were soaked in various salt solutions (in this study NaCl was used). The results of the study indicated that the application of stresses (both NaCl and PEG) induced retardation of growth attributes (measured in terms of shoot length, fresh weight, dry weight) and decrease in physiological attributes like total chlorophyll content, metabolites, photosynthetic and mitochondrial activity of the seedlings in all three V. radiata (L.) varieties. However, halopriming of the seeds could reduce the extent of decrease in these biological attributes. NaCl and PEG stress also caused increase in MDA content (a product of membrane lipid peroxidation) in all the varieties studied and this increase was significantly minimized under halopriming. From the present investigation it was evident that among the green gram varieties studied, Pusa Vishal, a NaCl tolerant variety showed enhanced tolerance to NaCl and PEG induced stress, when the seeds were subjected to halopriming followed by Pusa Ratna (stress sensitive variety). Pusa 9531 (drought tolerant variety) also showed positive halopriming effects but it was less significant when compared to other two varieties. It could be concluded that halopriming improved the drought and salinity stress tolerance potential of all varieties and it was significantly higher in the Pusa Vishal as compared to Pusa 9531 and Pusa Ratna.

  20. Expanding the repertoire of microsatellite markers for polymorphism studies in Indian accessions of mung bean (Vigna radiata L. Wilczek).

    Science.gov (United States)

    Shrivastava, Divya; Verma, Priyanka; Bhatia, Sabhyata

    2014-09-01

    Limited availability of validated, polymorphic microsatellite markers in mung bean (Vigna radiata), an important food legume of India, has been a major hurdle towards its improvement and higher yield. The present study was undertaken in order to develop a new set of microsatellite markers and utilize them for the analysis of genetic diversity within mung bean accessions from India. A GA/CT enriched library was constructed from V. radiata which resulted in 1,250 putative recombinant clones of which 850 were sequenced. SSR motifs were identified and their flanking sequences were utilized to design 328 SSR primer pairs. Of these, 48 SSR markers were employed for assessing genetic diversity among 76 mung bean accessions from various geographical locations in India. Two hundred and thirty four alleles with an average of 4.85 alleles per locus were detected at 48 loci. The polymorphic information content (PIC) per locus varied from 0.1 to 0.88 (average: 0.49 per locus). The observed and expected heterozygosities ranged from 0.40 to 0.95 and 0.40 to 0.81 respectively. Based on Jaccard's similarity matrix, a dendrogram was constructed using the unweighted pair-group method with arithmetic averages (UPGMA) analysis which revealed that one accession from Bundi, Rajasthan was clustered out separately while remaining accessions were grouped into two major clusters. The markers generated in this study will help in expanding the repertoire of the available SSR markers thereby facilitating analysis of genetic diversity, molecular mapping and ultimately broadening the scope for genetic improvement of this legume.

  1. Interdependence of plant water status with photosynthetic performance and root defense responses in Vigna radiata (L.) Wilczek under progressive drought stress and recovery.

    Science.gov (United States)

    Sengupta, Debashree; Guha, Anirban; Reddy, Attipalli Ramachandra

    2013-10-05

    The present study investigates the interdependence of plant water status with foliar and root responses in Vigna radiata L.Wilczek under progressive drought. Vegetatively-mature V. radiata plants were subjected to water withdrawal for 3 and 6days (D3 and D6, respectively) and then re-watered subsequently for 6days (6R) for stress-recovery. Changes in plant water status were expressed in terms of leaf and root moisture contents (LMC and RMC, respectively) and leaf relative water content (LRWC). Progressive drought caused apparent decrease in LRWC, LMC and RMC depicting significant level of dehydration of leaf and root tissues. Stomatal limitation alone could not account for the observed decrease in net CO2 assimilation rates (Pn) due to comparatively less decrease in sub-stomatal CO2 (Ci) concentrations with respect to other gas exchange parameters indicating possible involvement of non-stomatal limitations. Analysis of polyphasic chl a fluorescence kinetics during progressive drought showed decreased energy connectivity among PSII units as defined by a positive L-band with highest amplitude during D6. Efficiency of electron flux from OEC towards PSII acceptor side was not significantly affected during drought conditions as evidenced by the absence of a positive K-band. Increasing root-level water-limitation enforced a gradual oxidative stress through H2O2 accumulation and membrane lipid peroxidation in V. radiata roots exhibiting drastic enhancement of proline content and a significant but gradual increase in ascorbic acid content as well as guaiacol peroxidase activity under progressive drought. Expression analysis of Δ(1) pyrroline-5-carboxylate synthetase (P5CS) through real time PCR and enzyme activity studies showed a strong positive correlation between VrP5CS gene expression, enzyme activity and proline accumulation in the roots of V. radiata under progressive drought and recovery. Drought-induced changes in root moisture content (RMC) showed positive linear

  2. PRODUÇÃO DE CHAPAS DE MADEIRA COMPENSADA DE CINCO ESPÉCIES DE PINUS TROPICAIS

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2001-03-01

    Full Text Available Este trabalho teve como objetivo avaliar o potencial de utilização de 5 espécies de pinus tropicais para produção de painéis compensados. As espécies estudadas foram: Pinus caribaea, Pinus chiapensis, Pinus maximinoi, Pinus oocarpa, Pinus tecunumannii e Pinus taeda, sendo esta última espécie como testemunha. Foram produzidos compensados de 5 lâminas com resinas uréia-formaldeído e fenol-formaldeído. Os resultados de inchamento e recuperação em espessura foram estatisticamente iguais entre as espécies estudadas, com exceção para inchamento em espessura das chapas coladas com resina fenol-formaldeído. As chapas de Pinus maximinoi e Pinus oocarpa, apresentaram melhores resultados de módulos de elasticidade. Para o módulo de ruptura, as chapas de Pinus maxininoi, Pinus oocarpa e Pinus taeda, coladas com resina fenol-formaldeído, apresentaram valores estatisticamente superiores em relação às demais espécies. Quanto a resistência da linha de cola, as chapas de Pinus maximinoi, Pinus taeda e Pinus chiapensis, foram as que apresentaram melhor desempenho. Com base nos resultados gerais da pesquisa, pode-se destacar a potencialidade da madeira de Pinus maximinoi e Pinus oocarpa para produção de chapas de madeira compensada.

  3. Hydraulic architecture and tracheid allometry in mature Pinus palustris and Pinus elliottii trees.

    Science.gov (United States)

    Gonzalez-Benecke, C A; Martin, T A; Peter, G F

    2010-03-01

    Pinus palustris Mill. (longleaf pine, LL) and Pinus elliottii Engelm. var. elliottii (slash pine, SL) frequently co-occur in lower coastal plain flatwoods of the USA, with LL typically inhabiting slightly higher and better-drained microsites than SL. The hydraulic architecture and tracheid dimensions of roots, trunk and branches of mature LL and SL trees were compared to understand their role in species microsite occupation. Root xylem had higher sapwood-specific hydraulic conductivity (k(s)) and was less resistant to cavitation compared with branches and trunk sapwood. Root k(s) of LL was significantly higher than SL, whereas branch and trunk k(s) did not differ between species. No differences in vulnerability to cavitation were observed in any of the organs between species. Across all organs, there was a significant but weak trade-off between water conduction efficiency and safety. Tracheid hydraulic diameter (D(h)) was strongly correlated with k(s) across all organs, explaining >73% of the variation in k(s). In contrast, tracheid length (L(t)) explained only 2.4% of the variability. Nevertheless, for trunk xylem, k(s) was 39.5% higher at 20 m compared with 1.8 m; this increase in k(s) was uncorrelated with D(h) and cell-wall thickness but was strongly correlated with the difference in L(t). Tracheid allometry markedly changed between sapwood of roots, trunks and branches, possibly reflecting different mechanical constraints. Even though vulnerability to cavitation was not different for sapwood of roots, branches or the trunks of LL and SL, higher sapwood to leaf area ratio and higher maximum sapwood-specific hydraulic conductivity in roots of LL are functional traits that may provide LL with a competitive advantage on drier soil microsites.

  4. Produção de chapas de madeira compensada de cinco espécies de pinus tropicais

    Directory of Open Access Journals (Sweden)

    Setsuo Iwakiri

    2001-01-01

    Full Text Available This research was developed aiming at evaluating the feasibility of the use of 5 species of tropical pine to plywood manufacture. The following species were studied: Pinus caribaea, Pinus chiapensis, Pinus maximinoi, Pinus oocarpa, Pinus tecunumannii and Pinus taeda, being the last used as the referential species. Plywood were manufactured with 5 plies, bonded with ureaformaldheyde and fenol-formaldheyde resin. The results of thickness sweeling and recovering were the same for all species studied, with exception to thickness sweeling for the boards glued with fenolformaldheyde resin. The boards made from Pinus maximinoi and Pinus oocarpa, showed the higher values in modulus of elasticity. The boards of Pinus maximinoi, Pinus oocarpa and Pinus taeda, glued with fenol-formaldheyde resin, resulted in higher values of the modulus of rupture, in comparison to other species. For the glue line strength, the boards of Pinus maximinoi, Pinus taeda and Pinus chiapensis, showed the better results. Based on the general results of this research it, could be said that the Pinus maximinoi and Pinus oocarpa present the high potentiality to plywood manufacture.

  5. Construcción de Distribuciones Multivariadas con Marginales Dependientes Usando Cópulas en R

    OpenAIRE

    Jaramillo-Elorza, M. C; Lozano, J. A

    2014-01-01

    Las cópulas se han convertido en una herramienta popular para la construcción de modelos multivariados en campos donde la dependencia multivariada es de gran interés. El propósito de este trabajo es presentar las cópulas tanto en su concepto teórico, como en su implementación en el software estadístico R y profundizar en la construcción de distribuciones multivariadas con marginales dependientes, usando la clase mvdc del paquete copula, la cual permite utilizar varias y diferentes marginales ...

  6. Interação Humano - Computador usando Visão Computacional

    Directory of Open Access Journals (Sweden)

    Bernardo Bucher B. Barbosa

    2015-07-01

    Full Text Available Este trabalho visa estudar maneiras de se explorar a Interação Humano Computador, usando Visão Computacional. A idéia tem como objetivo um esforço para tornar o computador mais interativo com o usuário, sem a necessidade da compra de um hardware ou acessório específico para tal. O produto final deste trabalho em desenvolvimento é um software que contempla esta funcionalidade, tornando o computador mais interativo.

  7. PRODUÇÃO DE CHAPAS DE MADEIRA COMPENSADA DE CINCO ESPÉCIES DE PINUS TROPICAIS

    OpenAIRE

    Setsuo Iwakiri; Danielle Previdi Olandoski; Gabriela Leonhardt; Martha Andreia Brand

    2001-01-01

    Este trabalho teve como objetivo avaliar o potencial de utilização de 5 espécies de pinus tropicais para produção de painéis compensados. As espécies estudadas foram: Pinus caribaea, Pinus chiapensis, Pinus maximinoi, Pinus oocarpa, Pinus tecunumannii e Pinus taeda, sendo esta última espécie como testemunha. Foram produzidos compensados de 5 lâminas com resinas uréia-formaldeído e fenol-formaldeído. Os resultados de inchamento e recuperação em espessura foram estatisticamente iguais entre as ...

  8. Analgesic and Anti-Inflammatory Activity of Pinus roxburghii Sarg.

    Directory of Open Access Journals (Sweden)

    Dhirender Kaushik

    2012-01-01

    Full Text Available The Chir Pine, Pinus roxburghii, named after William Roxburgh, is a pine native to the Himalaya. Pinus roxburghii Sarg. (Pinaceae is traditionally used for several medicinal purposes in India. As the oil of the plant is extensively used in number of herbal preparation for curing inflammatory disorders, the present study was undertaken to assess analgesic and anti-inflammatory activities of its bark extract. Dried and crushed leaves of Pinus roxburghii Sarg. were defatted with petroleum ether and then extracted with alcohol. The alcoholic extract at the doses of 100 mg/kg, 300 mg/kg, and 500 mg/kg body weight was subjected to evaluation of analgesic and anti-inflammatory activities in experimental animal models. Analgesic activity was evaluated by acetic acid-induced writhing and tail immersion tests in Swiss albino mice; acute and chronic anti-inflammatory activity was evaluated by carrageenan-induced paw oedema and cotton pellet granuloma in Wistar albino rats. Diclofenac sodium and indomethacin were employed as reference drugs for analgesic and anti-inflammatory studies, respectively. In the present study, the alcoholic bark extract of Pinus roxburghii Sarg. demonstrated significant analgesic and anti-inflammatory activities in the tested models.

  9. A new C-methylated flavonoid glycoside from Pinus densiflora.

    Science.gov (United States)

    Jung, M J; Choi, J H; Chung, H Y; Jung, J H; Choi, J S

    2001-12-01

    A new C-methyl flavonol glycoside, 5,7,8,4'-tetrahydroxy-3-methoxy-6-methylflavone 8-O-beta-D-glucopyranoside (1), has been isolated from the needles of Pinus densiflora, together with kaempferol 3-O-beta-(6"-acetyl)-galactopyranoside.

  10. Impacts of prescribed fire on Pinus rigida Mill

    Science.gov (United States)

    Nicholas J. Carlo; Heidi J. Renninger; Kenneth L. Clark; Karina V.R. Schäfer

    2016-01-01

    A comparative analysis of the impacts of prescribed fire on three upland forest stands in the Northeastern Atlantic Plain, NJ, USA, was conducted. Effects of prescribed fire on water use and gas exchange of overstory pines were estimated via sap-flux rates and photosynthetic measurements on Pinus rigida Mill. Each study site had two sap-flux plots...

  11. Evaluation of genetic diversity of Portuguese Pinus sylvestris L.

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Genetics; Volume 92; Online resources. Evaluation of genetic diversity of Portuguese Pinus sylvestris L. populations based on molecular data and inferences about the future use of this germplasm. J. Cipriano A. Carvalho C. Fernandes M. J. Gaspar J. Pires J. Bento L. Roxo J. Louzada J. Lima- ...

  12. Growth process and diameter structure of Pinus tabulaeformis forest ...

    African Journals Online (AJOL)

    STORAGESEVER

    2009-10-19

    Oct 19, 2009 ... and sunny slope Pinus tabulaeformis forest were investigated in hilly loess-gully ... tabulaeformis, the main species of tree for forestation, ... Biomass in different artificial P. tabulaformis stands ..... The change of plant diversity during natural ... elements and stand biomass in forest communities in Hilly Loess.

  13. Genomic DNA extraction from sapwood of Pinus roxburghii for ...

    African Journals Online (AJOL)

    A method for extraction of genomic DNA from sapwood tissues of mature tall trees of Pinus roxburghii, where collection of needle tissues is extremely difficult has been standardized. The extracted DNA was comparable to that obtained from the needle tissue in terms of yield and purity. The yield of extracted DNA ranged ...

  14. Bishop pine (Pinus muricata) of inland Marin County, CA

    Science.gov (United States)

    Constance I. Millar

    1986-01-01

    The locations and characteristics of five, small, previously undescribed stands of bishop pine (Pinus muricata) in central Marin Co., California, are reported. Three stands lie on dry sites in the Kent Lake Drainage north of Mt. Tamalpais: San Geronimo Ridge, a spur ridge above Little Carson Cr., and Oat Hill. These stands are anomalous in occurring...

  15. Experiments in rooting bishop pine (Pinus muricata D. Don) cuttings

    Science.gov (United States)

    Constance I. Millar

    1987-01-01

    Presented here are results of rooting studies using hedges established from juvenile seedlings of "blue" and "green" foliaged bishop pine (Pinus muricata D. Don) from Mendocino and Sonoma Counties, California. Rootability, averaged over all clones and all setting dates, was 88%. The average time for 50% of the...

  16. A holistic approach to genetic conservation of Pinus strobiformis

    Science.gov (United States)

    K.M. Waring; R. Sniezko; B.A. Goodrich; C. Wehenkel; J.J. Jacobs

    2017-01-01

    Pinus strobiformis (southwestern white pine) is threatened by both a rapidly changing climate and the tree disease white pine blister rust, caused by an introduced fungal pathogen, Cronartium ribicola. We began a proactive program in ~2009 to sustain P. strobiformis that includes genetic conservation, research, and management strategies. Research...

  17. Evolutionary relationships of Slash Pine ( Pinus elliottii ) with its ...

    African Journals Online (AJOL)

    llozymes in bud tissue and monoterpene contents in xylem oleoresin of slash pine (Pinus elliottii) were analyzed from populations across the natural distribution, as well as those from other species in the AUSTRALES pines. Allozyme diversity measures of slash pine were similar to those found in other southern pines.

  18. Evaluation of seed production of scots pine ( Pinus sylvestris L ...

    African Journals Online (AJOL)

    This research was carried out to investigate seed production in a 13 years-old scots pine (Pinus sylvestris L.) clonal seed orchard, including 30 clones. Eight of cone and seed traits as number of fertile and infertile scales, cone volume, cone number, filled and empty seed number, seed efficiency and 1000 seed weight were ...

  19. evaluation of the leave and bud decoctions pinus halepensis mill ...

    African Journals Online (AJOL)

    31 déc. 2014 ... L'espèce Pinus halepensis Mill (ou pin d'Alep), appartenant à la famille des Pinaceae, a ... au moyen d'un moulin électrique à usage domestique (model Moulinex D5001) au niveau du ..... central ltaly Flavour Frag. J., 2003 ...

  20. Pinus ponderosa : A checkered past obscured four species

    Science.gov (United States)

    Ann Willyard; David S. Gernandt; Kevin Potter; Valerie Hipkins; Paula E. Marquardt; Mary Frances Mahalovich; Stephen K. Langer; Frank W. Telewski; Blake Cooper; Connor Douglas; Kristen Finch; Hassani H. Karemera; Julia Lefler; Payton Lea; Austin Wofford

    2016-01-01

    PREMISE OF THE STUDY: Molecular genetic evidence can help delineate taxa in species complexes that lack diagnostic morphological characters. Pinus ponderosa (Pinaceae; subsection Ponderosae ) is recognized as a problematic taxon: plastid phylogenies of exemplars were paraphyletic, and mitochondrial phylogeography suggested at...

  1. Growth models for Pinus patula in Angola | Delgado-Matas ...

    African Journals Online (AJOL)

    This study developed growth models for Pinus patula Schiede ex Schltdl. et Cham. for the Central Highlands of Angola for simulating the development of stand characteristics. The model set included dominant height, individual-tree diameter increment, individual-tree height and self-thinning models. The study was based ...

  2. Some physical and strength properties of immature Pinus patula ...

    African Journals Online (AJOL)

    A study was conducted to determine physical and strength properties of immature Pinus patula grown in Iringa and Njombe regions of Tanzania. Sample trees aged 5 to 15 years were collected from farmers' woodlots. The trees were categorized into 5 age classes: 5 - 7, 8 - 10, 11 - 12, 13 - 14 and 15 years. Four trees from ...

  3. Impact of the eocene on the evolution of Pinus L.

    Science.gov (United States)

    Constance I. Millar

    1993-01-01

    Pinus evolved in middle latitudes of the Northern Hemisphere in the middle Mesozoic. By the late Cretaceous pines had spread east and west throughout Laurasia, attaining high diversity in eastern Asia, the eastern United States, and western Europe, but having little representation at high northern latitudes. Changing climates in the early Tertiary...

  4. An interesting chemical polymorphism in Pinus sylvestris L.

    Directory of Open Access Journals (Sweden)

    Jerzy Szweykowski

    2014-01-01

    Full Text Available Intra- and interpopulational polymorphism in the production of phenolic compounds is described in Polish populations of Pinus sylvestris L. Two mutually exclusive forms of pine trees are present in changing proportions in all populations studied. This allows three groups of populations to be distinguished. The character of this differentiation is discussed.

  5. Percentile-based Weibull diameter distribution model for Pinus ...

    African Journals Online (AJOL)

    Using a site index equation and stem volume model developed for Pinus kesiya in the Philippines, a yield prediction system was created to predict the volume per ha (VPH) for each diameter class and, subsequently, the total volume of a stand. To evaluate the yield prediction system, the predicted mean VPH for each ...

  6. Natural parasitism of Diaphorina citri Kuwayama (Hemiptera, Psyllidae nymphs by Tamarixia radiata Waterston (Hymenoptera, Eulophidae in São Paulo orange groves

    Directory of Open Access Journals (Sweden)

    Paulo Eduardo Branco Paiva

    2012-12-01

    Full Text Available The psyllid Diaphorina citri Kuwayama 1908 has become the main citrus pest species in the state of São Paulo, Brazil, after the introduction of the huanglongbing or citrus greening. This study evaluated the parasitism of 3rd, 4th and 5th instar D. citri nymphs by Tamarixia radiata (Waterston, 1922 in citrus groves under a regimen of regular insecticide applications in ten producing regions: Araraquara, Barretos, Bauru, Botucatu, Franca, Itapetininga, Jaú, Limeira, Lins and São João da Boa Vista. Sixty-nine samples of new branches infested with nymphs of D. citri were collected from 2005 to 2008 in orange groves ranging from 1 to 20 years old, of the varieties Hamlin, Pera, Valencia and Natal. The parasitoid T. radiata is widely distributed in São Paulo orange groves, and was identified in 50 (72% of the samples, showing a mean parasitism rate of 12.4%. The highest parasitism rate was observed in the "summer" (from January through March, with a mean of 25.7%. Nymphal parasitism was above 90% in two samples. The probable causes of the variations in parasitism of D. citri by T. radiata are discussed.

  7. Growth and photosynthetic responses of two pine species (Pinus koraiensis and Pinus rigida) in a polluted industrial region in Korea

    International Nuclear Information System (INIS)

    Choi, D.S.; Kayama, M.; Jin, H.O.; Lee, C.H.; Izuta, T.; Koike, T.

    2006-01-01

    We investigated the effects of pollutants on two pine species (Pinus koraiensis and Pinus rigida) in an industrial region in Korea, using a physiological approach. The concentrations of fluorine (F) and chlorine (Cl) in the atmosphere, in precipitation and soil water at the damaged site were all significantly higher than at a control site. Moreover, the concentrations of F, Cl and Mn in pine needles were significantly higher, and essential elements and chlorophyll in needles were significantly lower at the damaged site than at the control site. The photosynthetic capacities, shoot length and survival statistics of needles of the two pines were all significantly reduced at the damaged site compared to the control site, especially P. rigida. Based on our comparison of photosynthetic responses and the concentrations of F, Cl and Mn in needles of the two pine species, P. koraiensis is more resistant to excess Mn in its needles than P. rigida. - Pinus koraiensis seems to be more pollution tolerant than Pinus rigida

  8. Carbon stable isotope-climate association in tree rings of Pinus pinaster and Pinus sylvestris in Mediterranean environments

    International Nuclear Information System (INIS)

    Bogino, Stella M; Bravo, Felipe

    2014-01-01

    Carbon isotope ratios, recorded as "1"3C/"1"2C variations in tree rings of woody species, are the result of physiological changes related to environmental conditions. The objective of this work was to analyze the association among carbon thirteen variability (δ"1"3C), climate variables and tree-ring growth of Pinus pinaster and Pinus sylvestris in central Spain. Pulverized woody material from the period 1975-1999 from four trees for each pine species was analyzed. To detect common patterns in δ"1"3C within each species and between δ"1"3C and growth indices, a principal component analysis (PCA) was performed. δ"1"3C of trees and the residual tree-ring chronologies were used at the PCA. Multilevel mixed linear models were applied between intrinsic water use efficiency (iWUE) and climate variables. Our results show an inverse significant correlation between δ"1"3C and tree-ring growth of both species. Winter and spring air moisture was negatively correlated with iWUE of Pinus pinaster. July maximum temperature was positively correlated with iWUE of Pinus sylvestris. As δ"1"3C is significantly related to climate and growth and it may be recommended as a valuable tool for tree growth dynamic analysis to withstand increasingly stressful climate conditions

  9. Time-of-Flight Adjustment Procedure for Acoustic Measurements in Structural Timber

    Science.gov (United States)

    Danbiel F. Llana; Guillermo Iñiguez-Gonzalez; Francisco Arriaga; Xiping Wang

    2016-01-01

    The effect of timber length on time-of-flight acoustic longitudinal measurements was investigated on the structural timber of four Spanish species: radiata pine (Pinus radiata D. Don), Scots pine (Pinus sylvestris L.), laricio pine (Pinus nigra Arn.), and maritime pine (Pinus pinaster Ait.). Time-of-flight longitudinal measurements were conducted on 120 specimens of...

  10. CLARIFICACIÓN DE AGUAS USANDO COAGULANTES POLIMERIZADOS: CASO DEL HIDROXICLORURO DE ALUMINIO

    Directory of Open Access Journals (Sweden)

    JUAN MIGUEL COGOLLO FLÓREZ

    2011-01-01

    Full Text Available En este artículo se realiza un estudio del proceso de clarificación en sistemas de tratamiento de aguas industriales usando un coagulante inorgánico polimerizado (hidroxicloruro de aluminio. Inicialmente, se establecen los elementos conceptuales más importantes de las etapas del proceso de clarificación (coagulación, floculación y sedimentación. Luego, se señalan los principales coagulantes convencionales utilizados en el tratamiento de aguas y se abordan los policloruros de aluminio (PAC´s como integrantes de una nueva generación de coagulantes alternativos cuyo uso se ha incrementado en las últimas décadas dado su mejor desempeño respecto a los coagulantes convencionales; se especifican los aspectos técnicos y operativos que se deben considerar al momento de implementar un proceso de clarificación de aguas usando un PAC como coagulante. Finalmente, se presentan datos comparativos de condiciones operacionales reales de un proceso de clarificación de aguas, producto de un trabajo previo, donde se remplazó un coagulante convencional (sulfato de aluminio por hidroxicloruro de aluminio, donde se corrobora el mejor desempeño del proceso luego del remplazo.

  11. Terpene chemodiversity of relict conifers Picea omorika, Pinus heldreichii, and Pinus peuce, endemic to Balkan.

    Science.gov (United States)

    Nikolić, Biljana; Ristić, Mihailo; Tešević, Vele; Marin, Petar D; Bojović, Srdjan

    2011-12-01

    Terpenes are often used as ecological and chemotaxonomic markers of plant species, as well as for estimation of geographic variability. Essential oils of relic and Balkan endemic/subendemic conifers, Picea omorika, Pinus heldreichii, and P. peuce, in central part of Balkan Peninsula (Serbia and Montenegro), on the level of terpene classes and common terpene compounds were investigated. In finding terpene combinations, which could show the best diversity between species and their natural populations, several statistical methods were applied. Apart from the content of different terpene classes (P. omorika has the most abundant O-containing monoterpenes and sesquiterpenes; P. heldreichii and P. peuce have the largest abundance of sesquiterpene and monoterpene hydrocarbons, resp.), the species are clearly separated according to terpene profile with 22 common compounds. But, divergences in their populations were established only in combination of several compounds (specific for each species), and they were found to be the results of geomorphologic, climatic, and genetic factors. We found similarities between investigated species and some taxa from literature with respect to terpene composition, possibly due to hybridization and phylogenetic relations. Obtained results are also important regarding to chemotaxonomy, biogeography, phylogeny, and evolution of these taxa. Copyright © 2011 Verlag Helvetica Chimica Acta AG, Zürich.

  12. A consensus genetic map for Pinus taeda and Pinus elliottii and extent of linkage disequilibrium in two genotype-phenotype discovery populations of Pinua taeda

    Science.gov (United States)

    Jared W. Westbrook; Vikram E. Chhatre; Le-Shin Wu; Srikar Chamala; Leandro Gomide Neves; Patricio Munoz; Pedro J. Martinez-Garcia; David B. Neale; Matias Kirst; Keithanne Mockaitis; C. Dana Nelson; Gary F. Peter; John M. Davis; Craig S. Echt

    2015-01-01

    A consensus genetic map for Pinus taeda (loblolly pine) and Pinus elliottii (slash pine) was constructed by merging three previously published P. taeda maps with a map from a pseudo-backcross between P. elliottii and P. taeda. The consensus map positioned 3856 markers via...

  13. Natural parasitism of Diaphorina citri Kuwayama (Hemiptera, Psyllidae nymphs by Tamarixia radiata Waterston (Hymenoptera, Eulophidae in São Paulo orange groves Parasitismo natural de ninfas de Diaphorina citri Kuwayama (Hemiptera, Psyllidae por Tamarixia radiata Waterston (Hymenoptera, Eulophidae em pomares de laranja em São Paulo

    Directory of Open Access Journals (Sweden)

    Paulo Eduardo Branco Paiva

    2012-12-01

    Full Text Available The psyllid Diaphorina citri Kuwayama 1908 has become the main citrus pest species in the state of São Paulo, Brazil, after the introduction of the huanglongbing or citrus greening. This study evaluated the parasitism of 3rd, 4th and 5th instar D. citri nymphs by Tamarixia radiata (Waterston, 1922 in citrus groves under a regimen of regular insecticide applications in ten producing regions: Araraquara, Barretos, Bauru, Botucatu, Franca, Itapetininga, Jaú, Limeira, Lins and São João da Boa Vista. Sixty-nine samples of new branches infested with nymphs of D. citri were collected from 2005 to 2008 in orange groves ranging from 1 to 20 years old, of the varieties Hamlin, Pera, Valencia and Natal. The parasitoid T. radiata is widely distributed in São Paulo orange groves, and was identified in 50 (72% of the samples, showing a mean parasitism rate of 12.4%. The highest parasitism rate was observed in the "summer" (from January through March, with a mean of 25.7%. Nymphal parasitism was above 90% in two samples. The probable causes of the variations in parasitism of D. citri by T. radiata are discussed.O psilídeo Diaphorina citri Kuwayama 1908 tornou-se a principal praga dos citros no estado de São Paulo após a introdução do huanglongbing ou grenning dos citros. Este estudo avaliou as proporções de ninfas de terceiro a quinto ínstares de D. citri parasitadas por Tamarixia radiata (Waterston, 1922 em pomares de laranja submetidos a pulverizações constantes de inseticidas em dez regiões produtoras, Araraquara, Barretos, Bauru, Botucatu, Franca, Itapetininga, Jaú, Limeira, Lins e São João da Boa Vista. Foram coletadas 69 amostras de ramos jovens infestados com ninfas de D. citri obtidas entre 2005 e 2008 em pomares de 1 a 20 anos de idade, das cultivares Hamilin, Pera, Valencia e Natal. O parasitóide T. radiata encontra-se amplamente distribuído em pomares de São Paulo, tendo sido observado em 50 amostras (72%, com taxa média de

  14. QUALIDADE DAS CHAPAS DE PARTÍCULAS AGLOMERADAS FABRICADAS COM RESÍDUOS DO PROCESSAMENTO MECÂNICO DA MADEIRA DE Pinus elliottii Engelm.

    Directory of Open Access Journals (Sweden)

    Leandro Calegari

    2005-09-01

    Full Text Available Este trabalho teve como objetivo avaliar as propriedades mecânicas de chapas de madeira aglomerada, confeccionados com resíduos de Pinus elliottii Engelm., resultantes do processamento mecânico da madeira. As chapas foram produzidas com dois tipos de resíduos, cavaco e maravalha, utilizados puros ou misturados. O adesivo utilizado foi à base de uréia-formaldeído em proporções de 4, 8 e 12% com base no peso seco das partículas de madeira, e as densidades nominais das chapas foram de 0,6 e 0,7 g/cm3. Foram avaliadas as propriedades de flexão estática, ligação interna e arrancamento de parafuso. Os valores das propriedades de flexão estática aumentaram com a proporção de maravalha, com a densidade da chapa, e com o teor de adesivo. Pode-se concluir que, usando as partículas do tipo maravalha, misturadas com maiores percentagens de adesivo e maiores densidades, são obtidas chapas com qualidade satisfatória.

  15. Qualidade das chapas de partículas aglomeradas fabricadas com resíduos do processamento mecânico da madeira de Pinus elliottii (Engelm..

    Directory of Open Access Journals (Sweden)

    Lourdes Patricia Elias Dacosta

    2010-08-01

    Full Text Available Este trabalho teve como objetivo avaliar as propriedades mecânicas de chapas de madeira aglomerada, confeccionados com resíduos de Pinus elliottii Engelm., resultantes do processamento mecânico da madeira. As chapas foram produzidas com dois tipos de resíduos, cavaco e maravalha, utilizados puros ou misturados. O adesivo utilizado foi à base de uréia-formaldeído em proporções de 4, 8 e 12% com base no peso seco das partículas de madeira, e as densidades nominais das chapas foram de 0,6 e 0,7 g/cm3. Foram avaliadas as propriedades de flexão estática, ligação interna e arrancamento de parafuso. Os valores das propriedades de flexão estática aumentaram com a proporção de maravalha, com a densidade da chapa, e com o teor de adesivo. Pode-se concluir que, usando as partículas do tipo maravalha, misturadas com maiores percentagens de adesivo e maiores densidades, são obtidas chapas com qualidade satisfatória.

  16. Reproductive toxicity of chromium in adult bonnet monkeys (Macaca radiata Geoffrey). Reversible oxidative stress in the semen

    International Nuclear Information System (INIS)

    Subramanian, Senthivinayagam; Rajendiran, Gopalakrishnan; Sekhar, Pasupathi; Gowri, Chandrahasan; Govindarajulu, Pera; Aruldhas, Mariajoseph Michael

    2006-01-01

    The present study was designed to test the hypothesis that oxidative stress mediates chromium-induced reproductive toxicity. Monthly semen samples were collected from adult monkeys (Macaca radiata), which were exposed to varying doses (50, 100, 200 and 400 ppm) of chromium (as potassium dichromate) for 6 months through drinking water. Chromium treatment decreased sperm count, sperm forward motility and the specific activities of antioxidant enzymes, superoxide dismutase and catalase, and the concentration of reduced glutathione in both seminal plasma and sperm in a dose- and duration-dependent manner. On the other hand, the quantum of hydrogen peroxide in the seminal plasma/sperm from monkeys exposed to chromium increased with increasing dose and duration of chromium exposure. All these changes were reversed after 6 months of chromium-free exposure period. Simultaneous supplementation of vitamin C (0.5 g/L; 1.0 g/L; 2.0 g/L) prevented the development of chromium-induced oxidative stress. Data support the hypothesis and show that chronic chromium exposure induces a reversible oxidative stress in the seminal plasma and sperm by creating an imbalance between reactive oxygen species and antioxidant system, leading to sperm death and reduced motility of live sperm

  17. Effects of chemical and biological pesticides on plant growth parameters and rhizospheric bacterial community structure in Vigna radiata

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Sunil; Gupta, Rashi; Sharma, Shilpi, E-mail: shilpi@dbeb.iitd.ac.in

    2015-06-30

    Highlights: • Non-target effects of pesticides employing qualitative and quantitative approaches. • Qualitative shifts in resident and active bacterial community structure. • Abundance of 16S rRNA gene and transcripts were reduced significantly. • Effects of biological pesticide similar to chemical pesticides on rhizospheric bacteria. - Abstract: With increasing application of pesticides in agriculture, their non-target effects on soil microbial communities are critical to soil health maintenance. The present study aimed to evaluate the effects of chemical pesticides (chlorpyrifos and cypermethrin) and a biological pesticide (azadirachtin) on growth parameters and the rhizospheric bacterial community of Vigna radiata. Qualitative and quantitative analysis by PCR-denaturing gradient gel electrophoresis (DGGE) and q-PCR, respectively, of the 16S rRNA gene and transcript were performed to study the impact of these pesticides on the resident and active rhizospheric bacterial community. While plant parameters were not affected significantly by the pesticides, a shift in the bacterial community structure was observed with an adverse effect on the abundance of 16S rRNA gene and transcripts. Chlorpyrifos showed almost complete degradation toward the end of the experiment. These non-target impacts on soil ecosystems and the fact that the effects of the biopesticide mimic those of chemical pesticides raise serious concerns regarding their application in agriculture.

  18. Effects of chemical and biological pesticides on plant growth parameters and rhizospheric bacterial community structure in Vigna radiata.

    Science.gov (United States)

    Singh, Sunil; Gupta, Rashi; Sharma, Shilpi

    2015-06-30

    With increasing application of pesticides in agriculture, their non-target effects on soil microbial communities are critical to soil health maintenance. The present study aimed to evaluate the effects of chemical pesticides (chlorpyrifos and cypermethrin) and a biological pesticide (azadirachtin) on growth parameters and the rhizospheric bacterial community of Vigna radiata. Qualitative and quantitative analysis by PCR-denaturing gradient gel electrophoresis (DGGE) and q-PCR, respectively, of the 16S rRNA gene and transcript were performed to study the impact of these pesticides on the resident and active rhizospheric bacterial community. While plant parameters were not affected significantly by the pesticides, a shift in the bacterial community structure was observed with an adverse effect on the abundance of 16S rRNA gene and transcripts. Chlorpyrifos showed almost complete degradation toward the end of the experiment. These non-target impacts on soil ecosystems and the fact that the effects of the biopesticide mimic those of chemical pesticides raise serious concerns regarding their application in agriculture. Copyright © 2015 Elsevier B.V. All rights reserved.

  19. Tolerance and toxicity levels of boron in mung bean (vigna radiata (l.) wilczek) cultivars at early growth stages

    International Nuclear Information System (INIS)

    Hasnain, A.; Mahmood, S.; Akhtar, S.; Malik, S.A.; Bashir, N.

    2011-01-01

    Boron (B) toxicity has been recognized as a serious problem in arid and semi arid regions of the world. This study was aimed to determine critical levels of B by studying phenotypic variation for B-tolerance/ toxicity at the germination and seedling stage in three mung bean (Vigna radiata) cultivars; M-6, M-8 and 96009. Boron levels ranging from 0-20 ppm were applied using Boric acid. Germination, growth and photosynthetic attributes were significantly (p<0.001) influenced by varying B levels. However, the cultivars were significantly invariable for germination, seedling height and leaf number. B levels (5-10 ppm) appeared to be nutritionally critical whereas, 15-20 ppm induced B toxicity. The toxicity was expressed in terms of reduction in plant's growth as well as by visible symptoms which included chlorosis and necrosis of the foliage. The present study also demonstrated variation in B tolerance at the seedling stage in these cultivars. Among the tested cultivars, M-6 and M-8 exhibited better growth responses as compared with 96009. Fresh biomass and shoot: root ratio appeared to serve as selection criteria for B tolerance. The study further suggested screening of cultivars/ accessions on a large scale to explore more diversity of traits as well as the use of biochemical markers for mechanistic understanding of B tolerance. (author)

  20. Effects of chemical and biological pesticides on plant growth parameters and rhizospheric bacterial community structure in Vigna radiata

    International Nuclear Information System (INIS)

    Singh, Sunil; Gupta, Rashi; Sharma, Shilpi

    2015-01-01

    Highlights: • Non-target effects of pesticides employing qualitative and quantitative approaches. • Qualitative shifts in resident and active bacterial community structure. • Abundance of 16S rRNA gene and transcripts were reduced significantly. • Effects of biological pesticide similar to chemical pesticides on rhizospheric bacteria. - Abstract: With increasing application of pesticides in agriculture, their non-target effects on soil microbial communities are critical to soil health maintenance. The present study aimed to evaluate the effects of chemical pesticides (chlorpyrifos and cypermethrin) and a biological pesticide (azadirachtin) on growth parameters and the rhizospheric bacterial community of Vigna radiata. Qualitative and quantitative analysis by PCR-denaturing gradient gel electrophoresis (DGGE) and q-PCR, respectively, of the 16S rRNA gene and transcript were performed to study the impact of these pesticides on the resident and active rhizospheric bacterial community. While plant parameters were not affected significantly by the pesticides, a shift in the bacterial community structure was observed with an adverse effect on the abundance of 16S rRNA gene and transcripts. Chlorpyrifos showed almost complete degradation toward the end of the experiment. These non-target impacts on soil ecosystems and the fact that the effects of the biopesticide mimic those of chemical pesticides raise serious concerns regarding their application in agriculture

  1. Arseno-sugars from brown kelp (Ecklonia radiata) as intermediates in cycling of Arsenic in a marine ecosystem

    Energy Technology Data Exchange (ETDEWEB)

    Edmonds, J S; Francesconi, K A

    1981-02-12

    The high concentration (relative to seawater) of arsenic in many marine animals eaten as human food has stimulated interest in the cycling of arsenic in the marine environment. Although arsenic is present in arsenobetaine ((CH/sub 3/)/sub 3/As/sup +/CH/sub 2/COO/sup -/) in the wester rock lobster (Panulirus cygnus), the dusky shark (Carcharhinus obscurus) and the school whiting (Sillago bassensis) it is not clear what intermediate stages are involved in the biosynthesis of this compound from arsenate, the major form of arsnenic in seawater. We now report the isolation of the two main arsenical constituents of the brown kelp, Ecklonia radiata, and their identification as a 2-hydroxy-3-sulphopropyl-5-deoxy-5-(dimethylarsenoso)furanoside and a 2,3-dihydroxypropyl-5-deoxy-5-(dimethylarsenoso)furanoside. A ..beta..-ribo structure for the sugar system is strongly indicated in each case. Ecklonia is the major organisms that concentrates arsenic in the coastal ecosystem to which the western rock lobster and school whiting belong. It is clear that the compounds described here could readily be further metabolized to arsenobetaine and may well be the source of arsenobetaine in marine fauna associated with the region.

  2. Yield and Yield Components of Vetch (Vigna radiata as Affected by the Use of Vermicompost and Phosphate Bio-fertilizer

    Directory of Open Access Journals (Sweden)

    Mohammad Mehdi Rahimi

    2016-06-01

    Full Text Available To evaluate the effects different levels of phosphate biofertilizer barvar-2 and vermi compost on yield and yield components of vetch plant (Vigna radiata L.in Yasouj a factorial experiments was performed in completely randomized design in crop year of 2013. Experimental treatments were phosphate biofertilizer barvar-2 at 3 levels (0, 50, 100 gram per hectare and vermicompost at 4 levels (0, 10, 20, 30 ton per hectare. In this study stem height, root length, biological yield, seed yield and harvest index was measured. ANOVA and comparison of means showed that vermicompost significantly increased stem height, economic and biological yields. Results, also, indicated that highest yield and biomass, 4.3 and 18.8 g/plant, observed respectively when 100 g/ha of barvar-2 and 30 t/ha of vermi compost were used. Using both of phosphate biofertilizer barvar-2 and vermicompost was better than their individnal usage. This indicates that combined use of these 2 factors would produce higher yield. It can be concluded that application of 100 g/ha of barvar-2 and 30 t/ha of vermicompost would a proper recommendation.

  3. Acetylcholineestarase-inhibiting alkaloids from Lycoris radiata delay paralysis of amyloid beta-expressing transgenic C. elegans CL4176.

    Directory of Open Access Journals (Sweden)

    Lijuan Xin

    Full Text Available The limited symptom relief and side effects of current Alzheimer's disease (AD medications warrant urgent discovery and study of new anti-AD agents. The "cholinergic hypothesis" of AD prompts us to search for plant-derived acetylcholineesterase (AChE inhibitors such as galanthamine that has been licensed in Europe for AD treatment. We used the unique amyloid β-expressing transgenic C. elegans CL4176, which exhibits paralysis when human Aβ1-42 is induced, to study two natural benzylphenethylamine alkaloids isolated from Lycoris radiata (L' Her. Herb, galanthamine and haemanthidine, and their synthetic derivatives 1,2-Di-O-acetyllycorine and 1-O-acetyllycorine for their anti-paralysis effects. Our data indicate that these Lycoris compounds effectively delay the paralysis of CL4176 worms upon temperature up-shift, and prolong the lives of these transgenic worms. Lycoris compounds were shown to significantly inhibit the gene expression of ace-1 and ace-2. Additionally, the Lycoris compounds may modulate inflammatory and stress-related gene expressions to combat the Aβ-toxicity in C. elegans.

  4. Economic evaluation (quantitative for Mung bean (Vigna radiata production in the Kamin Region, Sadat Shahr, Fars province

    Directory of Open Access Journals (Sweden)

    Mahmood Reza Sadikhani

    2016-11-01

    Full Text Available Although one of the biggest disadvantages of an economic evaluation, is volatility of price and instability of economic conditions, But for land use planning, economic considerations play a key role in land use and making decision. Cost is an important factor that encourage farmers to grow special crop. Thus, in addition to the qualitative and quantitative evaluation, Land Suitability can be based on net or gross profit per unit area of land to be assessed in economic terms. The economic evaluation is based on net income or gross income from the land. The purpose of this study was to assess the economic evaluation for part of Saadat Shahr (Kamin region for Mung bean (Vigna radiata. 8 drilled profiles in order to see the profiles were chosen and after collecting data on real output, the variable cost of producing the product and the critical values and estimated gross profit are calculated and land suitability classes were determined. The results showed that one unit has a moderate suitability (S2 and two units have suitable classes (S1 from separate units.

  5. The mitochondrial genome of the legume Vigna radiata and the analysis of recombination across short mitochondrial repeats.

    Directory of Open Access Journals (Sweden)

    Andrew J Alverson

    2011-01-01

    Full Text Available The mitochondrial genomes of seed plants are exceptionally fluid in size, structure, and sequence content, with the accumulation and activity of repetitive sequences underlying much of this variation. We report the first fully sequenced mitochondrial genome of a legume, Vigna radiata (mung bean, and show that despite its unexceptional size (401,262 nt, the genome is unusually depauperate in repetitive DNA and "promiscuous" sequences from the chloroplast and nuclear genomes. Although Vigna lacks the large, recombinationally active repeats typical of most other seed plants, a PCR survey of its modest repertoire of short (38-297 nt repeats nevertheless revealed evidence for recombination across all of them. A set of novel control assays showed, however, that these results could instead reflect, in part or entirely, artifacts of PCR-mediated recombination. Consequently, we recommend that other methods, especially high-depth genome sequencing, be used instead of PCR to infer patterns of plant mitochondrial recombination. The average-sized but repeat- and feature-poor mitochondrial genome of Vigna makes it ever more difficult to generalize about the factors shaping the size and sequence content of plant mitochondrial genomes.

  6. Accelerated Stem Growth Rates and Improved Fiber Properties of Loblolly Pine: Functional Analysis Of CyclinD from Pinus taeda

    Energy Technology Data Exchange (ETDEWEB)

    Dr. John Cairney, School of Biology and Institute of Paper Science and Technology @ Georgia Tech, Georgia Institute of Technology; Dr. Gary Peter, University of Florida; Dr. Ulrika Egertsdotter, Dept. of Forestry, Virgina Tech; Dr. Armin Wagner, New Zealand Forest Research Institute Ltd. (Scion Research.)

    2005-11-30

    divisions in the cambial meristem as expected. We isolated a promoter from a cambial specific gene and commenced development of transformation protocols for loblolly pine. Since our results show that cyclin D expression correlates with increased growth we continued with experiments to demonstrate the effect of cyclin overexpression upon tree growth. Vectors which constitutively express the cyclin D cDNA were constructed and transformed into a transgenic pine system through the collaboration with Forest Research, New Zealand. The transformation system for Pinus radiata is well established and we hoped to gain phenotypic information in a closely related pine, rather than await development of a robust loblolly pine transformation method. Transformation experiments were conducted by a biolistic method developed at Forest Research, NZ. A total of 78 transgenic embryogenic lines were generated and bulked up with a good representation of transgenic lines per construct. Transformed calli were originally identified by resistance to the antibiotic Geneticin contained in the medium. The transgenic nature of the selected lines was subsequently confirmed using histochemical GUS staining. To date, 10 out of 13 selected transgenic lines have produced embryos and we are currently harvesting the first transgenic plantlets. At present time 22 of those plantlets have been moved to GMO facilities. We will soon develop a strategy for assessing potential phenotypic differences between the transclones and non-transformed controls. Transgenic plants are being grown to a stage (approx. 1 year) when meaningful phenotypic evaluation can be conducted. The recent availability of 10,000 element loblolly pine cDNA microarray will permit the evaluation of cyclinD overexpression upon gene expression in transgenic Pinus.

  7. KAJIAN KONSERVASI Pinus merkusii strain Tapanuli DI SUMATERA

    Directory of Open Access Journals (Sweden)

    Hendi Suhendi

    2017-01-01

    Full Text Available Di Indonesia, Pinus yang tumbuh secara alami hanyalah Pinus merkusii di Sumatera yang terdiri dari strain Tapanuli, strain Kerinci dan strain Aceh. Berdasarkan persebarannya, strain Tapanuli tidak banyak dijumpai karena tercampur dengan jenis-jenis kayu daun lebar. Secara alami, strain Tapanuli ditemukan di Cagar Alam Dolok Sipirok dan Cagar Alam Dolok Saut. Dalam bentuk hutan tanaman, strain Tapanuli dibuat oleh masyarakat atau rakyat dengan anakan alam dan diambil secara cabutan di Tegakan Benih Dolok Tusam, dan sekarang sudah habis ditebang karena digantikan oleh tanaman kopi. Di wilayah kerja Dinas Kehutanan Propinsi Sumatera Utara hampir tidak pernah didapatkan informasi tentang keberadaan strain Tapanuli. Konservasi in situ dalam bentuk Cagar Alam perlu dilengkapi dengan konservasi ex situ. Sebagai langkah awal konservasi, terlebih dahulu perlu dikaji permudaan alamnya. Di samping itu, analisis kebijakan berkaitan dengan pentingnya eksplorasi dengan metode sensus pada semua kawasan konservasi di Sumatera perlu dipertimbangkan, dan pertemuan formal antar pengambil kebijakan di Departemen Kehutanan perlu direkomendasikan

  8. Sexual stability in the nearly dioecious Pinus johannis (Pinaceae).

    Science.gov (United States)

    Flores-Rentería, Lluvia; Molina-Freaner, Francisco; Whipple, Amy V; Gehring, Catherine A; Domínguez, C A

    2013-03-01

    Even though dioecy is a dominant sexual system among gymnosperms, little is known about its evolutionary history. Pinus johannis may represent a model system because unisexual and monoecious individuals compose its populations. The presence of unisexual individuals in other Pinus species is a consequence of sexual lability. Here we determined whether P. johannis represents the first example of a dioecious or nearly dioecious reproductive system in conifers by evaluating its sexual stability. • To assess the stability of sexual expression, we quantified the proportion of male vs. female reproductive structures produced by trees over multiple years and tested for the presence of sexual dimorphism. Sexual lability hypotheses were also examined by looking at the relationship between environmental factors and sexual expression and by comparing the reproductive behavior of P. johannis with its closest labile relative, P. edulis. • Pinus johannis is nearly dioecious: ~99% of individuals are unisexual or express a low proportion of the opposite gender with few changes in sexual expression through time. We found sexual dimorphism consistent with sexual stability. Sexual expression did not vary with tree size/age, abiotic environment, or herbivore removal, providing evidence against sexual lability. Individuals of P. johannis tended to produce only male or female strobili, whereas those of P. edulis were mainly monoecious with a gradient in the female to male strobili ratio. • This study represents the first report of a nearly stable dioecious Pinus species. The variety of sexual morphs coexisting in the same population makes P. johannis a model for studying the evolution of dioecy in gymnosperms.

  9. Genomic DNA extraction from sapwood of Pinus roxburghii for ...

    African Journals Online (AJOL)

    Ashish

    2013-02-22

    Feb 22, 2013 ... A method for extraction of genomic DNA from sapwood tissues of mature tall trees of Pinus roxburghii, .... DNA as a template. PCR was performed on a thermal cycler. (Biorad, Mycycler) incorporating 10 ng genomic DNA to a 25 µl reaction mix containing 1X Taq buffer, 3 mM MgCl2, 0.2 mM each of dNTPs ...

  10. MICROBIOTA OF PINUS POLLEN AS ADJUVANT FACTOR OF ALLERGY

    OpenAIRE

    Tetiana Shevtsova; Miroslava Kačániová; Jana Petrová; Ján Brindza; Kateryna Garkava

    2016-01-01

    Bacteria, their endotoxin and mold found on pollen can be a reason of respiratory symptoms in sensitized individuals. This question concerns an anemophilous pollen more acute. In this work quantitative by dilution plating method and qualitative microbial analysis by MALDI-TOF MS Biotyper of pollen and other plants organs of Pinus sylvestris L., P. nigra Arnold, P. mugo Turra, P. armandii Franch., P. wallichiana A.B. Jacks from Nitra, Slovakia are performed which shows quantitative and species...

  11. Photosynthesis and respiration in the needles of Pinus sibirica and Pinus pumila and their putative hybrids

    Directory of Open Access Journals (Sweden)

    A.P. Zotikova

    2013-12-01

    Full Text Available A putative interspecific hybridization in Pinaceae family was investigated. Very rarely the physiological methods were involved in hybridization processes that occurs in the hybrid zones. It is well known that in most gymnosperms, the plastid genome is inherited from the paternal component while the mitochondrion is inherited from the maternal one. Therefore functioning pattern of organelles in the hybrid plant can suggest parent, from which they were inherited. The aim of this study was to indirectly establish the inheritance energy-transducing organelles (mitochondria, chloroplast according to their functioning. Current year needles from Siberian Stone Pine (Pinus sibirica Du Tour and Japanese Stone Pine (Pinus pumila (Pall. Regel as parent species and their putative hybrids were collected from Baikal Region. The photosynthesis rate was determined by using the spectrophotometer. The study of emission CO2 under dark respiration of needle was conducted with laser optical-acoustic gasanalyzer. The quantity was measured at 1, 2 and 3 hour after experiment start. The rate of the photoreduction ferricyanide potassium was characterized by the primary photochemical processes activity at the level of photosystem II. Comparison of pure species was shown that Japanese Stone Pine had higher functional activity of chloroplast as compared with SiberianStone Pine in spite of the fact that they are growing in similar environment conditions. Two of three analyzed hybrids had decreasedactivity of their chloroplasts. Unfortunately, in this case we can't conclude if the chloroplasts were inherited from Siberian Stone Pine orfrom Japanese Stone Pine. Chloroplast activity of the third hybrid was approximately similar to that of Japanese Stone Pine suggesting thatits chloroplasts were inherited from this parent. Consequently, the Siberian Stone Pine and the Japanese Stone Pine were maternal and paternal, respectively parents of

  12. Biological invasion of Pinus ponderosa and Pinus contorta: case study of a forest plantation in Northwestern Patagonia; Invasion biologica de Pinus ponderosa y Pinus contorta: estudio de caso de una plantacion en la Patagonia noroccidental

    Energy Technology Data Exchange (ETDEWEB)

    Dezzotti, A.; Sbrancia, R.; Mortoro, A.; Monte, C.

    2009-07-01

    In the Southern Hemisphere, Pinus species from plantations can bring about processes of biological invasion that cause significant and permanent changes on the structure and functioning of surrounding natural ecosystems. The invasive character of Pinus ponderosa (P) and Pinus contorta (C) was examined for a 20-year old plantation located in the Alicura Forest Station (40 degree centigrade 40' S and 71 degree centigrade 00' W), through the analysis of abundance, age and spatial structures, and dispersal of natural regeneration. Seedlings and saplings were located largely within the plantation boundaries, and exhibited a density of 6.9 ind / ha (41 % for P and 59 % for C), a clustered spatial pattern with clumps dispersed not randomly, and a mean dispersal rate of 9.5 m / yr for P. ponderosa and 5.4 m / yr for P. contorta. Both species were invading the adjacent area, according to technical criteria based on ecological responses. However, regeneration niche is strongly hindering tree establishment and dispersal, probably due to high plant cover, presence of vertic soils, and absence of ectomycorrhizal fungi. These results can contribute to predict the capability of P. contorta and P. ponderosa to become invasive, in order to maximize the positive balance of forestry based on these species in northwestern Patagonia. (Author) 50 refs.

  13. SÍNTESIS DE OXITOCINA EN FASE SÓLIDA USANDO DERIVADOS DE TERBUTOXICARBONILO Y FLUORENILMETOXICARBONILO

    Directory of Open Access Journals (Sweden)

    Julio C Calvo

    2010-08-01

    Full Text Available La oxitocina, péptido cíclico cuya secuencia es CYIQNCPLG, fué el primer péptido de importancia biológica que pudo ser sintetizado. En este trabajo se compara la síntesis de la oxitocina usando resina p metilbenzhidrilamina (MBHA para la síntesis por estrategia t-Boc y resina Rink p-metilbenzhidrilamina (Rink MBHA para la síntesis por estrategia Fmoc, con altos rendimientos. El péptido crudo se ciclizó en una disolución acuosa de dimetilsulfóxido al 10%. La caracterización se llevó a cabo por espectrometría de masas y resonancia magnética nuclear, y se logró detectar la presencia de dos isómeros.

  14. Ectomycorrhizal diversity associated with Cedrus deodara and Pinus wallichiana in the Kashmir Himalaya, India.

    Science.gov (United States)

    Itoo, Zahoor Ahmad; Reshi, Zafar A

    2014-01-01

    The present study was undertaken to document the ectomycorrhizal diversity associated with the Cedrus deodara and Pinus wallichiana in the Kashmir Himalaya, India. The extensive field surveys carried out in the Kashmir Himalaya at five study sites resulted in the collection and identification of 76 potential ectomycorrhizal fungal species associated with the Cedrus deodara and Pinus wallichiana. Maximum 32 number of species were found associated with Pinus wallichiana, 19 with Cedrus deodara and 25 species were found growing in association with both the conifers. The present study reveals that Cedrus deodara and Pinus wallichiana in the Kashmir Himalaya, India harbour diverse ectomycorrhizal fungal species.

  15. Mineral Analysis of Pine Nuts (Pinus spp.) Grown in New Zealand.

    Science.gov (United States)

    Vanhanen, Leo P; Savage, Geoffrey P

    2013-04-03

    Mineral analysis of seven Pinus species grown in different regions of New Zealand; Armand pine ( Pinus armandii Franch), Swiss stone pine ( Pinus cembra L.), Mexican pinyon ( Pinus cembroides Zucc. var. bicolor Little), Coulter pine ( Pinus coulteri D. Don), Johann's pine ( Pinus johannis M.F. Robert), Italian stone pine ( Pinus pinea L.) and Torrey pine ( Pinus torreyana Parry ex Carrière), was carried out using an inductively coupled plasma optical emission spectrophotometer (ICP-OES) analysis. Fourteen different minerals (Al, B, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S and Zn) were identified in all seven varieties, except that no Al or Na was found in Pinus coulteri D. Don. New Zealand grown pine nuts are a good source of Cu, Mg, Mn, P and Zn, meeting or exceeding the recommended RDI for these minerals (based on an intake of 50 g nuts/day) while they supplied between 39%-89% of the New Zealand RDI for Fe. Compared to other commonly eaten tree-nuts New Zealand grown pine nuts are an excellent source of essential minerals.

  16. Molecular and analysis of a phenylalanine ammonia-lyase gene (LrPAL2) from Lycoris radiata.

    Science.gov (United States)

    Jiang, Yumei; Xia, Bing; Liang, Lijian; Li, Xiaodan; Xu, Sheng; Peng, Feng; Wang, Ren

    2013-03-01

    Phenylalanine ammonia-lyase (PAL), the first enzyme of phenylpropanoid biosynthesis, participates in the biosynthesis of flavonoids, lignins, stilbenes and many other compounds. In this study, we cloned a 2,326 bp full-length PAL2 gene from Lycoris radiata by using degenerate oligonucleotide primer PCR (DOP-PCR) and the rapid amplification of cDNA ends method. The cDNA contains a 2,124 bp coding region encoding 707 amino acids. The LrPAL2 shares about 77.0 % nucleic acid identity and 83 % amino acid identity with LrPAL1. Furthermore, genome sequence analysis demonstrated that LrPAL2 gene contains one intron and two exons. The 5' flanking sequence of LrPAL2 was also cloned by self-formed adaptor PCR (SEFA-PCR), and a group of putative cis-acting elements such as TATA box, CAAT box, G box, TC-rich repeats, CGTCA motif and TCA-element were identified. The LrPAL2 was detected in all tissues examined, with high abundance in bulbs at leaf sprouting stage and in petals at blooming stage. Besides, LrPAL2 drastically responded to MJ, SNP and UV, moderately responded to GA and SA, and a little increased under wounding. Comparison of LrPAL2 expression and LrPAL1 expression demonstrated that LrPAL2 can be more significantly induced than LrPAL1 under the above treatments, and LrPAL2 transcripts accumulated prominently at blooming stage, especially in petals, while LrPAL1 transcripts did not accumulated significantly at blooming stage. All these results suggested that LrPAL2 might play distinct roles in different branches of the phenylpropanoid pathway.

  17. Formation and action of lignin-modifying enzymes in cultures of Phlebia radiata supplemented with veratric acid

    International Nuclear Information System (INIS)

    Lundell, T.; Hatakka, A.; Leonowicz, A.; Rogalski, J.

    1990-01-01

    Transformation of veratric (3,4-dimethoxybenzoic) acid by the white rot fungus Phlebia radiata was studied to elucidate the role of ligninolytic, reductive, and demeth(ox)ylating enzymes. Under both air and a 100% O 2 atmosphere, with nitrogen limitation and glucose as a carbon source, reducing activity resulted in the accumulation of veratryl alcohol in the medium. When the fungus was cultivated under air, veratric acid caused a rapid increase in laccase (benzenediol:oxygen oxidoreductase; EC 1.10.3.2) production, which indicated that veratic acid was first demethylated, thus providing phenolic compounds for laccase. After a rapid decline in laccase activity, elevated lignin peroxidase (ligninase) activity and manganese-dependent peroxidase production were detected simultaneously with extracellular release of methanol. This indicated apparent demethoxylation. When the fungus was cultivated under a continuous 100% O 2 flow and in the presence of veratric acid, laccase production was markedly repressed, whereas production of lignin peroxidase and degradation of veratryl compounds were clearly enhanced. In all cultures, the increases in lignin peroxidase titers were directly related to veratryl alcohol accumulation. Evolution of 14 CO 2 from 3-O 14 CH 3 -and 4-O 14 CH 3 -labeled veratric acids showed that the position of the methoxyl substituent in the aromatic ring only slightly affected demeth(ox)ylation activity. In both cases, more than 60% of the total 14 C was converted to 14 CO 2 under air in 4 weeks, and oxygen flux increased the degradation rate of the 14 C-labeled veratric acids just as it did with unlabeled cultures

  18. Characterization of a novel Y2K-type dehydrin VrDhn1 from Vigna radiata.

    Science.gov (United States)

    Lin, Chia-Hui; Peng, Po-Hsin; Ko, Chia-Yun; Markhart, Albert H; Lin, Tsai-Yun

    2012-05-01

    A novel dehydrin gene (VrDhn1) was isolated from an embryo cDNA library of Vigna radiata (L.) Wilczek (mungbean) variety VC1973A. The intronless VrDhn1 gene encodes a protein belonging to the Y(2)K-type dehydrin family. VrDhn1 protein accumulated in embryos and cotyledons during seed maturation and disappeared 2 days after seed imbibition (DAI). The expression of VrDhn1 mRNA and accumulation of VrDhn1 protein were at high levels in mature seeds, but neither mRNA nor protein was detected in mungbean vegetative tissues under normal growth conditions. The VrDhn1 mRNA level was extremely high in mature seeds and decreased to ∼30% at 1 DAI, and was not detectable at ~7 DAI. Tissue dehydration, salinity and exogenous ABA markedly induced VrDhn1 transcripts in plants as measured by quantitative real-time reverse transcription-PCR (qRT-PCR). VrDhn1 protein was not detected using immunoblots in seedlings under stress treatments. In mature seeds or 1 DAI seedlings, VrDhn1 proteins were immunolocalized in the nucleus and cytoplasm. VrDhn1 exhibited low affinity for non-specific interaction with DNA using electrophoretic mobility shift assays (EMSAs), and the exogenous addition of Zn(2+) or Ni(2+) stimulated interaction. The His-tagged VrDhn1 (30.17 kDa) protein showed a molecular mass of 63.1 kDa on gel filtration, suggesting a dimer form. This is the first report showing that a Y(2)K-type VrDhn1 enters the nucleus and interacts with DNA during seed maturation.

  19. Combined efficacy of Vigna radiata (L. R. Wilczek and Amorphophallus paeoniifolius (Dennst. Nicolson on serum lipids in albino rats

    Directory of Open Access Journals (Sweden)

    P.B. Benil

    2017-09-01

    Full Text Available Coronary Artery Disease (CAD is a major killer disease throughout the world. Dyslipidemia is a major contributor to the risk of CAD. Several dietary articles traditionally used in India and other South Asian countries reduced dyslipidemia. The present study was undertaken to evaluate the combined effect of Mung bean (Vigna radiata and Elephant foot yam (Amorphophallus paeoniifolius on serum lipids and atherogenic indices in albino rats and to compare it with a standard drug Cholestyramine. Thirty healthy albino rats of both sexes (150–200 g were randomized to 5 groups of 6 animals each. The grouping were done based on the following criteria: Group I: Normal Control Group, Group II: (Standard Group: Cholestyramine resin 5 mg/kg bw, Group III: (Half Dose Group: Drug powder at 540 mg/kg bw, Group IV: (Effective Dose Group: Drug powder at 1080 mg/kg bw, and Group V: (Double Dose Group: Drug powder at 2160 mg/kg bw. Lipid profile was estimated at the beginning and after 30 days of treatment. The Effective and Double doses of the drug reduced Total cholesterol along with levels of Triglycerides, Low density lipoprotein and Very low density lipoprotein levels significantly (p < 0.01 along with a significant (p < 0.01 increase in high density lipoproteins (HDL in rats. There was also significant (p < 0.01 improvement in atherogenic indices like Castelli Risk Index I, Non HDL C/HDL, Castelli risk Index II, TG/HDL, Atherogenic coefficient and Atherogenic Index of Plasma. The combination of powdered sprouted mung bean and yam powder have excellent lipid lowering potential.

  20. Competencia morfogénica de embriones maduros de Pinus radiata cultivados in vitro y su relación con la posición del cono en el árbol

    OpenAIRE

    Sánchez-Olate, Manuel; Zapata Valenzuela, Jaime; Sáez Delgado, Patricia; Ríos Leal, Darcy; Spiercolli Henríquez, Scarlette; Alvarez Maldini, Carolina; Delaveau, Catherine; Pereira Cancino, Guillermo

    2008-01-01

    La micropropagación de material embrionario colectado de árboles adultos constituye una alternativa importante en la propagación masiva de fenotipos selectos, adecuados para los programas de clonación implementados por las empresas forestales. Utilizando esta técnica, ejes embrionarios maduros extraídos desde conos lignificados ubicados a diferentes alturas en el árbol (zonas basal, media y apical) fueron introducidos in vitro con el objeto de analizar la posible relación entre el estado fisi...

  1. Influence of gap-scale disturbance on developmental and successional pathways in Quercus-Pinus stands

    Science.gov (United States)

    T.A. Weber; J.L. Hart; C. Schweitzer; D.C. Dey

    2014-01-01

    Quercus-Pinus forests of the eastern USA cover millions of hectares and span a variety of ecoregions. Understanding the influence of natural disturbance on developmental and successional pathways is important for managers that wish to sustain Pinus spp. in these mixtures. Quantifying developmental and successional patterns in this...

  2. Development of site index curves for Pinus kesiya in the Philippines ...

    African Journals Online (AJOL)

    Development of site index curves for Pinus kesiya in the Philippines. RIC Lumbres, YJ Lee, YO Seo, FG Calora JR. Abstract. This study was conducted to develop a height–age growth model and site index curves for site quality evaluation of old secondary-growth stands of Pinus kesiya in the northern Philippines.

  3. Resistance to white pine blister rust in Pinus flexilis and P

    Science.gov (United States)

    Anna W. Schoettle; Richard A. Sniezko; Angelia Kegley; Jerry Hill; Kelly S. Burns

    2010-01-01

    The non-native fungus Cronartium ribicola, that causes white pine blister rust (WPBR), is impacting or threatening limber pine, Pinus flexilis, and Rocky Mountain bristlecone pine, Pinus aristata. In the Southern Rockies, where the rust invasion is still expanding, we have the opportunity to be proactive and prepare the landscape for invasion. Genetic...

  4. CONTINUIDADE ESPACIAL DE CARACTERÍSTICAS DENDROMÉTRICAS DE PINUS (Pinus pinaster Aiton E DE ATRIBUTOS DO SOLO / SPATIAL CONTINUITY OF DENDROMETRICS CHARACTERISTICS OF PINUS (Pinus pinaster Aiton AND SOIL ATTRIBUTES

    Directory of Open Access Journals (Sweden)

    GLÉCIO M. SIQUEIRA

    2009-04-01

    Full Text Available O objetivo deste trabalho foi avaliar a relação e a continuidade espacial de características dendrométricas de pinus (Pinus pinaster Aiton e de atributos do solo. As amostragens foram realizadas em uma área de 0,25 ha cultivada com Pinus pinaster em Muras (Província de Lugo, Galícia, Espanha. Os atributos amostrados foram: o diâmetro da base das árvores (DB; o diâmetro a 0,30 m acima da base (D30; o diâmetro a altura do peito (a 1,3 m de altura, DAP, altura das árvores (ALT, profundidade do perfil do solo (PROF e a condutividade elétrica aparente do solo a 0,3 m de profundidade (CEa-H e a 1,5 m de profundidade (CEa-V. O uso da geoestatística permitiu caracterizar a variabilidade espacial de todos os atributos em estudo, com exceção da profundidade da rocha que apresentou efeito pepita puro. Os dados apresentaram moderada razão de dependência espacial entre amostras. Os mapas de variabilidade espacial não demonstram uma relação entre os mapas dos atributos de solo e planta estudados.Palavras-Chave: geoestatística; silvicultura de precisão; condutividade elétrica aparente do solo.

  5. Pine weevil (Hylobius abietis) antifeedants from lodgepole pine (Pinus contorta).

    Science.gov (United States)

    Bratt, K; Sunnerheim, K; Nordenhem, H; Nordlander, G; Langström, B

    2001-11-01

    Pine weevils (Hylobius abietis) fed less on bark of lodgepole pine (Pinus contorta) than on bark of Scots pine (P. sylvestris). Two pine weevil antifeedants, ethyl trans-cinnamate and ethyl 2,3-dibromo-3-phenyl-propanoate, were isolated from bark of lodgepole pine. These two compounds significantly reduced pine weevil feeding in a laboratory bioassay. In field assays, the second compound significantly decreased pine weevil damage on planted seedlings. Ethyl 2,3-dibromo-3-phenylpropanoate has not previously been reported as a natural product.

  6. Pinus pinaster Knot: A Source of Polyphenols against Plasmopara viticola.

    Science.gov (United States)

    Gabaston, Julien; Richard, Tristan; Cluzet, Stéphanie; Palos Pinto, Antonio; Dufour, Marie-Cécile; Corio-Costet, Marie-France; Mérillon, Jean-Michel

    2017-10-11

    Pine knot extract from Pinus pinaster byproducts was characterized by UHPLC-DAD-MS and NMR. Fourteen polyphenols divided into four classes were identified as follows: lignans (nortrachelogenin, pinoresinol, matairesinol, isolariciresinol, secoisolariciresinol), flavonoids (pinocembrin, pinobanksin, dihydrokaempferol, taxifolin), stilbenes (pinosylvin, pinosylvin monomethyl ether, pterostilbene), and phenolic acids (caffeic acid, ferulic acid). The antifungal potential of pine knot extract, as well as the main compounds, was tested in vitro against Plasmopara viticola. The ethanolic extract showed a strong antimildew activity. In addition, pinosylvins and pinocembrin demonstrated significant inhibition of zoospore mobility and mildew development. These findings strongly suggest that pine knot is a potential biomass that could be used as a natural antifungal product.

  7. Genetic diversity and gene exchange in Pinus oocarpa, a Mesoamerican pine with resistance to the pitch canker fungus (Fusarium circinatum)

    Science.gov (United States)

    W.S. Dvorak; K.M. Potter

    2009-01-01

    Eleven highly polymorphic microsatellite markers were used to determine the genetic structure and levels of diversity in 51 natural populations of Pinus oocarpa across its geographic range of 3000 km in Mesoamerica. The study also included 17 populations of Pinus patula and Pinus tecunumanii chosen for their resistance or susceptibility to the pitch canker fungus based...

  8. Changes in some biophysical and biochemical parameters of mungbean [vigna radiata (L.) wilczek] grown on chromium-contaminated soils treated with solid tea wastage

    International Nuclear Information System (INIS)

    Azmat, R.; Akhtar, H.

    2010-01-01

    The success of solid tea wastage treatment technology in remediating chromium (111) contamination in the soil has been demonstrated on growth of Vigna radiata. The present research was designed to study the effect of chromium (Cr3/sup +/) on plant growth, potassium (K), phosphorus (P), protease activity and proline profile of Vigna radiata as a bio indicator in the presence and absence of the solid tea surface as a bio sorbent to control the mobility of Cr3/sup +/ in the soil. Results showed toxic effects of Cr3/ sup +/ on plant growth and development, which include high protease activity with prominent proline and decreased potassium and phosphorus contents at elevated concentration of metal. Proline content is the only amino acid that accumulates to a greater extent in the leaves of plants under stress. An increase in proline contents in leaves, stem and root with high concentration of Cr3/s sup +/ gets reduced in a solid tea wastage amended plants. Metabolic alteration by Cr3/ sup +/exposure and their control by solid tea wastage already described in the first report, showed direct effect on enzymes or other metabolites or by its ability to generate reactive oxygen species which may cause oxidative stress. It is suggested that the plant can grow under chromium stress if some suitable adsorbent (like tea wastage) is mixed with the soil which can protect the plants from the phyto toxicity of Cr 3/sup +/ by altering various metabolic processes. (author)

  9. Gill tissue reactions in walleye Stizostedion vitreum vitreum and common carp Cyprinus carpio to glochidia of the freshwater mussel Lampsilis radiata siliquoidea

    Science.gov (United States)

    Waller, D.L.; Mitchell, L.G.

    1989-01-01

    The glochidia of many freshwater mussels, which are obligate parasites on the gills, fins, and other body parts of specific fishes, attach to a suitable host, become encapsulated, and develop to the free-living juvenile stage. Using light and electron microscopy we compared gill tissue reactions in a suitable host (walleye Stizostedion vitreum vitreum) and unsuitable host (common carp Cyprinus carpio) infected with Lampsilis radiata siliquoidea. Encapsulation of glochidia on walleye gills was completed by 6 h post-infection at 20 to 22°C. Capsular formation and compaction were accompanied by a general increase in epithelioid cells. Fibrotic material appeared in capsules at about 48 h and virtually filled capsular cells from about Day 5 to Day 11 post-infection. Liberation of juvenile mussels was accompanied by thinning of the capsule from about Day 11 to Day l7. Although glochidia attached to the gills of common carp, few became encapsulated. By 48 h post-infection, preliminary capsular growth was evident and necrotic cells and cellular debris appeared at the edges of the growth. However, all glochidia were sloughed from carp gills by 60 h. Host specificity of L. radiata siliquoidea apparently depended on a combination of the attachment response of glochidia, differences in the encapsulation process, and tissue reactions in the fish.

  10. Composition and structure of Pinus koraiensis mixed forest respond to spatial climatic changes.

    Science.gov (United States)

    Zhang, Jingli; Zhou, Yong; Zhou, Guangsheng; Xiao, Chunwang

    2014-01-01

    Although some studies have indicated that climate changes can affect Pinus koraiensis mixed forest, the responses of composition and structure of Pinus koraiensis mixed forests to climatic changes are unknown and the key climatic factors controlling the composition and structure of Pinus koraiensis mixed forest are uncertain. Field survey was conducted in the natural Pinus koraiensis mixed forests along a latitudinal gradient and an elevational gradient in Northeast China. In order to build the mathematical models for simulating the relationships of compositional and structural attributes of the Pinus koraiensis mixed forest with climatic and non-climatic factors, stepwise linear regression analyses were performed, incorporating 14 dependent variables and the linear and quadratic components of 9 factors. All the selected new models were computed under the +2°C and +10% precipitation and +4°C and +10% precipitation scenarios. The Max Temperature of Warmest Month, Mean Temperature of Warmest Quarter and Precipitation of Wettest Month were observed to be key climatic factors controlling the stand densities and total basal areas of Pinus koraiensis mixed forest. Increased summer temperatures and precipitations strongly enhanced the stand densities and total basal areas of broadleaf trees but had little effect on Pinus koraiensis under the +2°C and +10% precipitation scenario and +4°C and +10% precipitation scenario. These results show that the Max Temperature of Warmest Month, Mean Temperature of Warmest Quarter and Precipitation of Wettest Month are key climatic factors which shape the composition and structure of Pinus koraiensis mixed forest. Although the Pinus koraiensis would persist, the current forests dominated by Pinus koraiensis in the region would all shift and become broadleaf-dominated forests due to the dramatic increase of broadleaf trees under the future global warming and increased precipitation.

  11. The Combined Intervention with Germinated Vigna radiata and Aerobic Interval Training Protocol Is an Effective Strategy for the Treatment of Non-Alcoholic Fatty Liver Disease (NAFLD and Other Alterations Related to the Metabolic Syndrome in Zucker Rats

    Directory of Open Access Journals (Sweden)

    Garyfallia Kapravelou

    2017-07-01

    Full Text Available Metabolic syndrome (MetS is a group of related metabolic alterations that increase the risk of developing non-alcoholic fatty liver disease (NAFLD. Several lifestyle interventions based on dietary treatment with functional ingredients and physical activity are being studied as alternative or reinforcement treatments to the pharmacological ones actually in use. In the present experiment, the combined treatment with mung bean (Vigna radiata, a widely used legume with promising nutritional and health benefits that was included in the experimental diet as raw or 4 day-germinated seed flour, and aerobic interval training protocol (65–85% VO2 max has been tested in lean and obese Zucker rats following a 2 × 2 × 2 (2 phenotypes, 2 dietary interventions, 2 lifestyles factorial ANOVA (Analysis of Variance statistical analysis. Germination of V. radiata over a period of four days originated a significant protein hydrolysis leading to the appearance of low molecular weight peptides. The combination of 4 day-germinated V. radiata and aerobic interval training was more efficient compared to raw V. radiata at improving the aerobic capacity and physical performance, hepatic histology and functionality, and plasma lipid parameters as well as reverting the insulin resistance characteristic of the obese Zucker rat model. In conclusion, the joint intervention with legume sprouts and aerobic interval training protocol is an efficient treatment to improve the alterations of glucose and lipid metabolism as well as hepatic histology and functionality related to the development of NAFLD and the MetS.

  12. The Combined Intervention with Germinated Vigna radiata and Aerobic Interval Training Protocol Is an Effective Strategy for the Treatment of Non-Alcoholic Fatty Liver Disease (NAFLD) and Other Alterations Related to the Metabolic Syndrome in Zucker Rats.

    Science.gov (United States)

    Kapravelou, Garyfallia; Martínez, Rosario; Nebot, Elena; López-Jurado, María; Aranda, Pilar; Arrebola, Francisco; Cantarero, Samuel; Galisteo, Milagros; Porres, Jesus M

    2017-07-19

    Metabolic syndrome (MetS) is a group of related metabolic alterations that increase the risk of developing non-alcoholic fatty liver disease (NAFLD). Several lifestyle interventions based on dietary treatment with functional ingredients and physical activity are being studied as alternative or reinforcement treatments to the pharmacological ones actually in use. In the present experiment, the combined treatment with mung bean ( Vigna radiata ), a widely used legume with promising nutritional and health benefits that was included in the experimental diet as raw or 4 day-germinated seed flour, and aerobic interval training protocol (65-85% VO₂ max) has been tested in lean and obese Zucker rats following a 2 × 2 × 2 (2 phenotypes, 2 dietary interventions, 2 lifestyles) factorial ANOVA (Analysis of Variance) statistical analysis. Germination of V. radiata over a period of four days originated a significant protein hydrolysis leading to the appearance of low molecular weight peptides. The combination of 4 day-germinated V. radiata and aerobic interval training was more efficient compared to raw V. radiata at improving the aerobic capacity and physical performance, hepatic histology and functionality, and plasma lipid parameters as well as reverting the insulin resistance characteristic of the obese Zucker rat model. In conclusion, the joint intervention with legume sprouts and aerobic interval training protocol is an efficient treatment to improve the alterations of glucose and lipid metabolism as well as hepatic histology and functionality related to the development of NAFLD and the MetS.

  13. Variantes del problema del cartero mixto que se pueden resolver usando programación lineal

    Directory of Open Access Journals (Sweden)

    Francisco Javier Zaragoza Martínez

    2012-07-01

    Full Text Available Dada una gráfica mixta y conexa con costos en sus aristas y arcos, el problema del cartero mixto consiste en encontrar un circuito cerrado de la gráfica mixta que recorra sus aristas y arcos a costo mínimo. Se sabe que este problema es NP-duro. Sin embargo, bajo ciertas condiciones adicionales, el problema se puede resolver en tiempo polinomial usando programación lineal, en otras palabras, los poliedros correspondientes son enteros. Algunas de estas condiciones son: la gráfica mixta es serie paralelo o la gráfica mixta tiene grado total par en todos sus vértices. Además, mostramos que si agregamos la restricción adicional de que cada arista se recorra exactamente una vez entonces el problema se puede resolver en tiempo polinomial si el conjunto de arcos forma un bosque. Palabras clave: Gráfica mixta, problema de cartero, programación lineal. Mathematics Subject Classification: 05C45, 90C35.

  14. Root architecture and wind-firmness of mature Pinus pinaster.

    Science.gov (United States)

    Danjon, Frédéric; Fourcaud, Thierry; Bert, Didier

    2005-11-01

    This study aims to link three-dimensional coarse root architecture to tree stability in mature timber trees with an average of 1-m rooting depth. Undamaged and uprooted trees were sampled in a stand damaged by a storm. Root architecture was measured by three-dimensional (3-D) digitizing. The distribution of root volume by root type and in wind-oriented sectors was analysed. Mature Pinus pinaster root systems were organized in a rigid 'cage' composed of a taproot, the zone of rapid taper of horizontal surface roots and numerous sinkers and deep roots, imprisoning a large mass of soil and guyed by long horizontal surface roots. Key compartments for stability exhibited strong selective leeward or windward reinforcement. Uprooted trees showed a lower cage volume, a larger proportion of oblique and intermediate depth horizontal roots and less wind-oriented root reinforcement. Pinus pinaster stability on moderately deep soils is optimized through a typical rooting pattern and a considerable structural adaptation to the prevailing wind and soil profile.

  15. Modelagem do crescimento e de biomassa individual de Pinus

    Directory of Open Access Journals (Sweden)

    Ana Beatriz Schikowski

    2013-09-01

    Full Text Available Este estudo tem como objetivo testar modelos matemáticos para estimativas de biomassa de diferentes compartimentos de Pinus spp., a partir de variáveis de fácil mensuração. Os dados utilizados são provenientes de plantios localizados no centro sul do estado do Paraná. Foram utilizados dados de peso seco total e parcial de 35 árvores de Pinus spp., obtidos por meio do método destrutivo direto. De cada árvore amostrada foram medidos também o CAP (circunferência à altura do peito e a altura total. Os modelos para estimativa de biomassa de folhagem não apresentaram bom desempenho, verificado pelos indicadores de ajuste. Entretanto, para os compartimentos: galhos, raízes, casca, fuste e para biomassa total, os ajustes apresentaram elevados valores de R² e baixos valores de Syx%. O modelo de crescimento de Richards obteve melhor desempenho que os demais testados para a estimativa da biomassa total.

  16. The complete plastid genome of Bunge's pine Pinus bungeana (Pinaceae).

    Science.gov (United States)

    Li, Zhong-Hu; Zhu, Juan; Yang, Yi-Xin; Yang, Jie; He, Jing-Wen; Zhao, Gui-Fang

    2016-07-01

    The complete nucleotide sequence of Bunge's pine Pinus bungeana Zucc. ex Endl. chloroplast genome (cp DNA) was determined in this study. The cpDNA was 117 861 bp in length, containing a pair of 475 bp inverted repeat regions (IRa and IRb), which were separated by large and small single copy regions (LSC and SSC) of 65 373 and 51 538 bp, respectively. The cpDNA contained 111 genes, including 71 protein-coding genes (71 PCG species), 4 ribosomal RNA genes (4 rRNA species) and 36 tRNA genes (32 tRNA species). In these genes, 13 harbored a single intron and 1 (ycf3) contained a couple of introns. The overall AT content of Bunge's pine cpDNA is 61.2%, while the corresponding values of the LSC, SSC and IR regions are 61.9%, 60.2% and 62.5%, respectively. A phylogenetic reconstruction based on the maximum parsimony analysis suggested that all the sampled Pinus species clustered a monophyletic clade and have a high bootstrap support, and the cpDNA of P. bungeana is closely related to that of congeneric P. gerardiana.

  17. Chemical composition, antimicrobial, insecticidal, phytotoxic and antioxidant activities of Mediterranean Pinus brutia and Pinus pinea resin essential oils.

    Science.gov (United States)

    Ulukanli, Zeynep; Karabörklü, Salih; Bozok, Fuat; Ates, Burhan; Erdogan, Selim; Cenet, Menderes; Karaaslan, Merve Göksin

    2014-12-01

    Essential oils of the resins of Pinus brutia and Pinus pinea were evaluated for their biological potential. Essential oils were characterized using GC-MS and GC/FID. in vitro antimicrobial, phytotoxic, antioxidant, and insecticidal activities were carried out using the direct contact and the fumigant assays, respectively. The chemical profile of the essential oils of the resins of P. pinea and P. brutia included mainly α-pinene (21.39% and 25.40%), β-pinene (9.68% and 9.69%), and caryophyllene (9.12% and 4.81%). The essential oils of P. pinea and P. brutia exerted notable antimicrobial activities on Micrococcus luteus and Bacillus subtilis, insecticidal activities on Ephestia kuehniella eggs, phytotoxic activities on Lactuca sativa, Lepidium sativum, and Portulaca oleracea, as well as antioxidant potential. Indications of the biological activities of the essential oils suggest their use in the formulation of ecofriendly and biocompatible pharmaceuticals. Copyright © 2014 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

  18. Molecular response to water stress in two contrasting Mediterranean pines (Pinus pinaster and Pinus pinea).

    Science.gov (United States)

    Perdiguero, Pedro; Barbero, María Del Carmen; Cervera, María Teresa; Collada, Carmen; Soto, Alvaro

    2013-06-01

    Adaptation to water stress has determined the evolution and diversification of vascular plants. Water stress is forecasted to increase drastically in the next decades in certain regions, such as in the Mediterranean basin. Consequently, a proper knowledge of the response and adaptations to drought stress is essential for the correct management of plant genetic resources. However, most of the advances in the understanding of the molecular response to water stress have been attained in angiosperms, and are not always applicable to gymnosperms. In this work we analyse the transcriptional response of two emblematic Mediterranean pines, Pinus pinaster and Pinus pinea, which show noticeable differences in their performance under water stress. Using microarray analysis, up to 113 genes have been detected as significantly induced by drought in both species. Reliability of expression patterns has been confirmed by RT-PCR. While induced genes with similar profiles in both species can be considered as general candidate genes for the study of drought response in conifers, genes with diverging expression patterns can underpin the differences displayed by these species under water stress. Most promising candidate genes for drought stress response include genes related to carbohydrate metabolism, such as glycosyltransferases or galactosidases, sugar transporters, dehydrins and transcription factors. Additionally, differences in the molecular response to drought and polyethylene-glycol-induced water stress are also discussed. Copyright © 2013 Elsevier Masson SAS. All rights reserved.

  19. Levels and sources of PAHs in selected sites from Portugal: biomonitoring with Pinus pinea and Pinus pinaster needles.

    Science.gov (United States)

    Ratola, Nuno; Amigo, José Manuel; Alves, Arminda

    2010-04-01

    Pine needle samples from two pine species (Pinus pinaster Ait. and Pinus pinea L.) were collected at 29 sites scattered throughout Portugal, in order to biomonitor the levels and trends of 16 polycyclic aromatic hydrocarbons (PAHs). The values obtained for the sum of all PAHs ranged from 76 to 1944 ng/g [dry weight (dw)]. Despite the apparent matrix similarities between both pine species, P. pinaster needles revealed higher mean entrapment levels than P. pinea (748 and 399 ng/g (dw) per site, respectively). The urban and industrial sites have the highest average of PAH incidence [for P. pinea, 465 and 433 ng/g (dw) per site, respectively, and for P. pinaster, 1147 and 915 ng/g (dw)], followed by the rural sites [233 ng/g and 711 ng/g (dw) per site, for P. pinea and P. pinaster, respectively]. The remote sites, both from P. pinaster needles, show the least contamination, with 77 ng/g (dw) per site. A predominance of 3-ring and 4-ring PAHs was observed in most samples, with phenanthrene having 30.1% of the total. Naphthalene prevailed in remote sites. Rainfall had no influence on the PAHs levels, but there was a relationship between higher wind speeds and lower concentrations. PAH molecular ratios revealed the influence of both petrogenic and pyrogenic sources.

  20. Impact of solar activity on growth of pine trees (Pinus cembra: 1610 - 1970; Pinus pinaster: 1910 -1989)

    Science.gov (United States)

    Surový, P.; Dorotovič, I.; Karlovský, V.; Rodrigues, J. C.; Rybanský, M.; Fleischer, P.

    2010-12-01

    In this work we have focused on the analysis of the data on the annual growth of cembra pine (Pinus cembra) grown in the Kôprová dolina Valley in the High Tatra Mountains. The database covers the period of 1406 - 1970, however, the sunspot data (minima and maxima) at the NGDC web site are only available since 1610. Moreover, reliable sunspot data are only available since 1749. The results of this analysis agree with the observation made in our previous work, i.e. there is a negative impact of high SA on the pine tree growth. However, it should be noted that statistical significance of the results is low. We also applied wavelet analysis to the data on the tree growth evolution, with the results indicating growth variations' period of about 20 years (duration of approximately two solar cycles or one magnetic cycle, respectively). A negative impact of the SA was also observed in growth of a 90 year-old maritime pine tree (Pinus pinaster) grown in northern Portugal. The width of the annual rings was smaller in the years of maximum SA; furthermore, it was found that it is the latewood growth that it is affected while the earlywood growth is not, and consequently the latewood additions also show a significative negative correlation with SA.

  1. Characterization of Phenolic Compounds in Pinus laricio Needles and Their Responses to Prescribed Burnings

    Directory of Open Access Journals (Sweden)

    Lila Ferrat

    2007-07-01

    Full Text Available Fire is a dominant ecological factor in Mediterranean-type ecosystems. Management strategies include prescribed (controlled burning, which has been used in the management of several species, such as Pinus nigra ssp laricio var. Corsicana, a pine endemic to Corsica of great ecological and economic importance. The effects of prescribed burning on Pinus laricio have been little studied. The first aim of this study was to characterize total and simple phenolic compounds in Pinus laricio. The second aim was to understand: i the short term (one to three months and medium term (three years effects of prescribed burning, and ii the effects of periodic prescribed burning on the production of phenolic compounds in Pinus laricio. The first result of this study is the presence of total and simple phenolic compounds in the needles of Pinus laricio. 3-Vanillyl propanol is the major compound. After a prescribed burning, the synthesis of total phenolic compounds increases in Pinus laricio for a period of three months. Total phenolic compounds could be used as bioindicators for the short-term response of Pinus laricio needles to prescribed burning. Simple phenolic compounds do not seem to be good indicators of the impact of prescribed burning because prescribed burnings are low in intensity.

  2. Characterization of phenolic compounds in Pinus laricio needles and their responses to prescribed burnings.

    Science.gov (United States)

    Cannac, Magali; Pasqualini, Vanina; Greff, Stéphane; Fernandez, Catherine; Ferrat, Lila

    2007-07-30

    Fire is a dominant ecological factor in Mediterranean-type ecosystems. Management strategies include prescribed (controlled) burning, which has been used in the management of several species, such as Pinus nigra ssp laricio var. Corsicana, a pine endemic to Corsica of great ecological and economic importance. The effects of prescribed burning on Pinus laricio have been little studied. The first aim of this study was to characterize total and simple phenolic compounds in Pinus laricio. The second aim was to understand: i) the short term (one to three months) and medium term (three years) effects of prescribed burning, and ii) the effects of periodic prescribed burning on the production of phenolic compounds in Pinus laricio. The first result of this study is the presence of total and simple phenolic compounds in the needles of Pinus laricio. 3-Vanillyl propanol is the major compound. After a prescribed burning, the synthesis of total phenolic compounds increases in Pinus laricio for a period of three months. Total phenolic compounds could be used as bioindicators for the short-term response of Pinus laricio needles to prescribed burning. Simple phenolic compounds do not seem to be good indicators of the impact of prescribed burning because prescribed burnings are low in intensity.

  3. Density determination in Pino Radiata (D.Don) samples using 59.5 keV gamma radiation attenuation; Determinacion de densidad en muestras de Pino Radiata (D. Don) mediante atenuacion de radiacion gamma de 59.5 KeV

    Energy Technology Data Exchange (ETDEWEB)

    Dinator, Maria I; Morales, Jose R; Aliaga, Nelson [Chile Univ., Santiago (Chile). Dept. de Fisica; Karsulovic, Jose T; Sanchez, Jaime; Leon, Adolfo [Chile Univ., Santiago (Chile). Dept. de Tecnologia de la Madera

    1997-12-31

    A non destructive method to determine wood samples density is presented. The photon mass attenuation coefficient in samples of Pino radiata (D.Don) was measured at 59.5 keV with a radioactive source of Am-241. The value of 0.192 {+-} 0.002 cm{sup 2}/g was obtained with a gamma spectroscopy system and later used on the determination of the mass density in sixteen samples of the same species. Comparison of these results with those of gravimetric method through a linear regression showed a slope of 1.001 and a correlation factor of 0.94. (author). 3 refs., 4 figs.

  4. Productivity and adaptation of Pinus in the north litoral of Bahia

    International Nuclear Information System (INIS)

    Barros Ferraz, E.S. de; Rezende, M.A.

    1986-01-01

    The productivity and climatic adaptation of two species of Pinus in the north litoral of Bahia State has been studied through the variability of wood density in a comercial plantation. The gamma-ray attenuation method of the 100 mCi 241-Am source, 59,6 KeV, was used for density variability analysis. The results show that Pinus caribaea hondurensis is better adapted to the region than Pinus caribaea caribaea, with a superior mean productivity of 26% at 8 years. (Author) [pt

  5. Un estudio sobre el desarrollo del pensamiento aleatorio usando recursos educativos abiertos

    Directory of Open Access Journals (Sweden)

    Yenny Patricia Pinzón Triana

    2015-04-01

    Full Text Available Este estudio presenta los resultados de un proyecto de investigación sobre la implementación de la enseñanza probabilística con recursos educativos abiertos (REA, diseñados en la plataforma Edmodo, y que está dirigido a estudiantes de tercer grado de educación básica secundaria de Bogotá, Colombia, y Tuxtepec, México. El fin era evidenciar sus preconceptos, nociones y evaluar el resultado de la instrucción en términos de su pensamiento probabilístico y dar respuesta a la interrogante ¿cuál es el efecto de la instrucción en probabilidad usando Edmodo en estudiantes de tercer grado de educación básica secundaria respecto a la valoración de fenómenos aleatorios de la vida cotidiana, sus conjeturas y la toma de decisiones? Se empleó un enfoque cualitativo a partir del método de estudio de casos, desde el análisis particular a lo general (Stake, 2005. Para la implementación, se consideró el estudio de Fishbein (1975 sobre el desarrollo del pensamiento probabilístico y el de Marzano (2000, relacionado con las dimensiones del aprendizaje. Estos elementos sirvieron para establecer el impacto del uso de los REA, en especial el de la plataforma Edmodo en el desarrollo de competencias en los estudiantes. Los instrumentos consistieron en una prueba de entrada, actividades de apoyo interactivo desde Edmodo y una prueba de salida para determinar los niveles de aprendizaje; mediante triangulación de datos, se evidenciaron alcances de niveles adecuados de desempeño acordes con los requerimientos de los estándares nacionales e internacionales.

  6. AUTOMATIZACIÓN DE LA ARQUITECTURA DE COMPONENTES GENÉRICOS USANDO UML

    Directory of Open Access Journals (Sweden)

    Noel Fuentes Ramírez

    2006-04-01

    Full Text Available

     

    La arquitectura de componentes genéricos permite chequear la consistencia interna de sus elementos arquitectónicos (componentes y conectores a partir de las relaciones internas en sus respectivas estructuras, que pueden ser de inclusión y transformación. También permite verificar la conexión entre componentes y conectores a partir de las relaciones de transformación entre sus interfaces respectivas. Las ideas que se presentan aquí constituyen una propuesta para la automatización de la descripción de esta arquitectura usando el lenguaje de modelado unificado (UML a partir de la descripción formal de sus diagramas de clases y de secuencia, así como para el chequeo de la consistencia. En este artículo se muestra la aplicación de esta propuesta mediante una extensión de la herramienta Visual Paradigm, por medio de un módulo de software conectable.

  7. Pirólise catalítica do PEBD usando como catalisador a vermiculita modificada

    Directory of Open Access Journals (Sweden)

    Franciel Aureliano Bezerra

    2016-01-01

    Full Text Available Resumo O polietileno de baixa densidade (PEBD é um dos polímeros mais usados atualmente, e a grande quantidade desse polímero produzida resulta em toneladas de resíduos, que necessitam ser tratados. Neste trabalho foi realizada a pirólise termocatalítica do PEBD usando como catalisador a argila vermiculita modificada, como alternativa para o tratamento dos resíduos. A argila foi tratada com solução de ácido nítrico a diferentes concentrações e calcinada a 400 °C. Os materiais foram caracterizados por técnicas de difratometria de raios X, termogravimetria, adsorção de nitrogênio e espectroscopia de energia dispersiva. A pirólise térmica e termocatalítica foi realizada em um micro reator acoplado com GC/MS, a 500 °C. O intuito da pirólise de resíduos poliméricos é a obtenção de hidrocarbonetos leves (C<16, que possam ser empregados na indústria química e petroquímica, através de quebras na cadeia polimérica. Os resultados foram satisfatórios, com aumento no rendimento para hidrocarbonetos leves ao empregar os catalisadores chegando a 71,4% de produtos com C<16, enquanto a pirólise térmica resultou apenas de 25,8%.

  8. Desarrollo de un horno solar para el secado de plantas y vegetales usando control difuso

    Directory of Open Access Journals (Sweden)

    Oscar G. Ibarra Manzano

    2012-05-01

    Full Text Available Recientemente, el aprovechamiento de la energía solar en el deshidratado de productos agrícolas se ha vuelto cada día más común debido a los altos rendimientos en los productos post-cosecha. La inversión en tecnologías propias para contribuir con los productores del sector agroalimentario es un factor importante para el desarrollo de las cadenas produc­tivas de nuestro país. En este trabajo se presenta el desarrollo de un horno solar para el secado de plantas y vegetales utilizando control difuso. Este es un sistema térmicamente controlado que permite disminuir el tiempo de secado de varios días a unas horas. Se reali­zaron pruebas de secado usando flor de jamaica, en las cuales se pudo disminuir el tiempo de secado de cuatro días a aproximadamente 5 h. Se presentan tanto la parte de diseño conceptual, como resultados experimentales del mismo. Los resultados obtenidos permiten ver la viabilidad del diseño propuesto.Recently, the use of solar energy in the dehydration of agricultural products is becomingmore common as high yields in the post-harvest products. Investment in technologies forcontributing to the producers of food products is an important factor for the development ofthe productive chains of our country. This paper presents the development of a solar oven fordrying plants and vegetables using fuzzy control. This is a heat-controlled system that allowsdecreasing the drying time from several days to hours. Drying tests were conducted usingjamaica flower, which could decrease the drying time from four days to about 5 h. We presentboth the conceptual design of the experimental results. The results obtained allow us to seethe feasibility of the proposed design.

  9. Quality of Pinus elliottii sawn timber from tapped forests

    Directory of Open Access Journals (Sweden)

    Pedro Henrique Gonzalez de Cademartori

    2012-12-01

    Full Text Available This study aimed to characterize the quality of Pinus elliottii sawn timber extracted from tapped forests and processed in a sawmill in São José do Norte (RS. Four butt logs and four upper logs for each of the three existing diameter grades were selected and sawed. The wood pieces were analyzed after sawing and after kiln drying. The presence of knots, which occurred due to the absence of forest management and influenced the qualitative classification of the wood pieces, was observed mainly in the samples from upper logs. The process of resin tapping contributed to a higher incidence of resin pockets in the samples from butt logs, also influencing the qualitative classification of the samples. The appearance of drying defects did not modify the classification of the wood samples from butt and upper logs.

  10. Protein synthesis in the embryo of Pinus thunbergii seed, 2

    International Nuclear Information System (INIS)

    Yamamoto, Naoaki; Sasaki, Satohiko.

    1977-01-01

    14 C-Amino acid incorporating activity in the absence of exogenous mRNA was found in a cell-free system from embryos of light-germinated Pinus thunbergii seeds, but not in that from dark-imbibed seed embryos. Template activity in the cell-free system from the light-germinated seed embryos was observed in the ribosome fraction, especially the polyribosome fraction, but not in the 100,000 x g supernatant fraction (s100). These facts suggest that the nature of the block in protein synthesis during the imbibition of seeds in the dark is due to the lack or inactivity of mRNA. The s100 from light-germinated seed embryos was found to be less active in amino acid incorporation than that from dark-imbibed seed embryos. (auth.)

  11. Urbanization in China drives soil acidification of Pinus massoniana forests

    Science.gov (United States)

    Huang, Juan; Zhang, Wei; Mo, Jiangming; Wang, Shizhong; Liu, Juxiu; Chen, Hao

    2015-09-01

    Soil acidification instead of alkalization has become a new environmental issue caused by urbanization. However, it remains unclear the characters and main contributors of this acidification. We investigated the effects of an urbanization gradient on soil acidity of Pinus massoniana forests in Pearl River Delta, South China. The soil pH of pine forests at 20-cm depth had significantly positive linear correlations with the distance from the urban core of Guangzhou. Soil pH reduced by 0.44 unit at the 0-10 cm layer in urbanized areas compared to that in non-urbanized areas. Nitrogen deposition, mean annual temperature and mean annual precipitation were key factors influencing soil acidification based on a principal component analysis. Nitrogen deposition showed significant linear relationships with soil pH at the 0-10 cm (for ammonium N (-N), P greatly contributed to a significant soil acidification occurred in the urbanized environment.

  12. NUTRIENTS CONCENTRATION AND RETRANSLOCATION IN THE Pinus taeda L. NEEDLES

    Directory of Open Access Journals (Sweden)

    Márcio Viera

    2010-03-01

    Full Text Available Aiming at evaluating nutrients concentration and retranslocation in the Pinus taeda L. needles, this study was developed in two stands, in native grass area and in second rotation area, with same species and same age (7.5 years old in Cambará do Sul, RS. The needles were collected in plants in four orthogonal points (South, North, East and West, sampled new needles, mature needles and old needles. The material was dried in a stove, milled and chemically analyzed (macro and micronutrients. The concentrations of N, P, K, B, Cu and Zn had decreased, of Ca, Fe and Mn increased, and the Mg and S have remained constant with the age of the needles. The retranslocation rate (old-new needles was more than 50% for most nutrients, except for Mn and Fe, showed that cumulative effect and the Ca reference element.

  13. Green Synthesis of Silver Nanoparticles Using Pinus eldarica Bark Extract

    Directory of Open Access Journals (Sweden)

    Siavash Iravani

    2013-01-01

    Full Text Available Recently, development of reliable experimental protocols for synthesis of metal nanoparticles with desired morphologies and sizes has become a major focus of researchers. Green synthesis of metal nanoparticles using organisms has emerged as a nontoxic and ecofriendly method for synthesis of metal nanoparticles. The objectives of this study were production of silver nanoparticles using Pinus eldarica bark extract and optimization of the biosynthesis process. The effects of quantity of extract, substrate concentration, temperature, and pH on the formation of silver nanoparticles are studied. TEM images showed that biosynthesized silver nanoparticles (approximately in the range of 10–40 nm were predominantly spherical in shape. The preparation of nano-structured silver particles using P. eldarica bark extract provides an environmentally friendly option, as compared to currently available chemical and/or physical methods.

  14. Anatomic Study of Female Sterility of Pinus tabulaeformis Carr.

    Institute of Scientific and Technical Information of China (English)

    Cheng Pengjun; Li Fenglan; Zheng Caixia

    2003-01-01

    The anatomic research on the mutant clone of Pinus tabulaeformis Carr. in the seed orchard in Xingcheng, LiaoningProvince was carried out. The female cone of the mutant clone looked like normal, but its ovules degenerated in the early stage. Thispaper tries to find out the reason and time of ovule abortion. It seems that the ovule abortion is probably caused by female sterilitybecause the microspores of this mutant clone were normal. Through the serial observations on the one-year-old macrosporangiatesand the ovules of two-year-old female cones of mutant and normal clone, it is found that the reason of ovule abortion in mutant cloneis the failure of the mitosis of free nuclei in the female gametophyte, and the time is about in the early April.

  15. Needles of Pinus halepensis as biomonitors of bioaerosol emissions.

    Directory of Open Access Journals (Sweden)

    Amandine Galès

    Full Text Available We propose using the surface of pine trees needles to biomonitor the bioaerosol emissions at a composting plant. Measurements were based on 16S rRNA gene copy numbers of Saccharopolyspora rectivirgula, a bioindicator of composting plant emissions. A sampling plan was established based on 29 samples around the emission source. The abundance of 16S rRNA gene copies of S. rectivirgula per gram of Pinus halepensis needles varied from 104 to 102 as a function of the distance. The signal reached the background level at distances around the composting plant ranging from 2 km to more than 5.4 km, depending on the local topography and average wind directions. From these values, the impacted area around the source of bioaerosols was mapped.

  16. Effect of soaking and fermentation on content of phenolic compounds of soybean (Glycine max cv. Merit) and mung beans (Vigna radiata [L] Wilczek).

    Science.gov (United States)

    María Landete, José; Hernández, Teresa; Robredo, Sergio; Dueñas, Montserrat; de Las Rivas, Blanca; Estrella, Isabel; Muñoz, Rosario

    2015-03-01

    Mung beans (Vigna radiata [L] Wilczek) purchased from a Spanish company as "green soybeans", showed a different phenolic composition than yellow soybeans (Glycine max cv. Merit). Isoflavones were predominant in yellow soybeans, whereas they were completely absent in the green seeds on which flavanones were predominant. In order to enhance their health benefits, both types of bean were subjected to technological processes, such as soaking and fermentation. Soaking increased malonyl glucoside isoflavone extraction in yellow beans and produced an increase in apigenin derivatives in the green beans. Lactobacillus plantarum CECT 748 T fermentation produced an increase in the bioactivity of both beans since a conversion of glycosylated isoflavones into bioactive aglycones and an increase of the bioactive vitexin was observed in yellow and green beans, respectively. In spite of potential consumer confusion, since soybean and "green soybean" are different legumes, the health benefits of both beans were enhanced by lactic fermentation.

  17. Nitrogen fixation by mung bean (Vigna radiata L.) under field conditions in the Philippines as quantified by 15N isotope dilution

    International Nuclear Information System (INIS)

    Rosales, C.M.; Rivera, F.; Hautia, R.A.; Del Rosario, E.

    1995-05-01

    Nitrogen fixation by five mung bean genotypes (Vigna radiata L.) was estimated using two reference crops at two locations in the Philippines. The percentage of N derived from fixation and the amount of N-fixed ranged from 64 to 87% and 43 to 85 kg N/ha respectively at one location and from 36.6 to 72% and 21 to 85 kg N/ha at another location using cotton as reference crop. Maize was not a good reference crop. The highest mung bean seed yields obtained were 1.99 t/ha and 0.86 t/ha in the two locations. As to residual benefits, corn dry matter seeds yield were higher when grown following N 2 -fixing mung bean than after non-fixing corn or cotton. (author). 25 refs., 1 fig., 5 tabs

  18. Isolation and screening of rhizobia for auxin biosynthesis and growth promotion of mung bean (Vigna radiata L. seedlings under axenic conditions

    Directory of Open Access Journals (Sweden)

    Muhammad Ashfaq Anjum, Zahir Ahmad Zahir, Muhammad Arshad and Muhammad Ashraf

    2011-04-01

    Full Text Available A series of screening experiments to evaluate the effectiveness of rhizobia for producing auxins and improvegrowth and nodulation of mungbean (Vigna radiata L. were carried out under axenic conditions. Forty fouriolatess of rhizobia were isolated using standard procedures. Auxin biosynthesis by these rhizobial isolates wasdetermined in the absence and presence of L-Trp, a physiological precursor of auxins. Rhizobial isolates variedwidely in auxins biosynthesis capabilities. On the basis of auxins biosynthesis, a pouch experiment was conductedfor screening thirty four efficient isolates of rhizobia for the growth promotion of mung bean. Results of pouch studyshowed that inoculation with selected rhizobial isolates increased the root /shoot length, fresh, and dry shoot weightof mung bean up to 33, 59, 71, 148, 107 and 188%, respectively, over untreated control. Further studies are neededunder glasshouse and field conditions for confirmation of these results.

  19. Evaluation of ischemic damage of the corticospinal tract by diffusion tensor MRI. Utility in predicting functional outcome of corona radiata infarcts

    International Nuclear Information System (INIS)

    Tanaka, Hideki; Matsuno, Akira; Okubo, Toshiyuki; Nakaguchi, Hiroshi; Murakami, Mineko; Ono, Seiichi; Takeuchi, Masato

    2011-01-01

    Motor impairment is one of the most frequent symptoms among stroke patients and often leads to post-stroke dependency, so evaluation of motor symptoms and underlining corticospinal tract (CST) damage is of prime importance. Motor impairment, ischemic lesion by diffusion weighted MRI, and clinical outcome were assessed in 15 acute to early subacute corona radiata infarct patients. Motor impairment was graded severe: limb movement synergy level, moderate: selective muscle activity possible and mild: isolated movements are well coordinated. Outcome at the time of discharge was assessed by modified Rankin Scale (mRS). Diffusion tensor MRI (GE Signa Excite system 1.5 T, Echo Planar Imaging, MPG 15) was conducted at 2.3±2.2 days from the onset of the clinical symptoms. CST was delineated 3-dimensionally with dTV.II.SR and Volume-one 1.72. CST-FA (fractional anisotropy) ratio and CST-Area % were calculated at the slice where CST-infarct overlap was maximal. CST-FA ratio and CST-Area % showed good correlation to motor impairment at presentation. Patients with severe motor impairment had lower CST-FA ratio and CSF-Area % than those with moderate or mild. CST-FA ratio was 0.73±0.22 in patients with poor clinical outcome (mRS 3-6) and 0.93±0.09 with good clinical outcome (mRS 0-2) (p=0.038). Diffusion tensor MRI is useful in evaluating ischemic CST damage and predicting functional outcome in patients with corona radiata infarcts in the acute to subacute stage. (author)

  20. The potential of L. scoparium, K. robusta and P. radiata to mitigate N-losses in silvopastural systems.

    Science.gov (United States)

    Esperschuetz, J; Balaine, N; Clough, T; Bulman, S; Dickinson, N M; Horswell, J; Robinson, B H

    2017-06-01

    Silvopastoral systems aim to enhance economic, cultural and social principles by sustainably combining forest management with agriculture. In these typically high-nitrogen (N) environments, plant species selection can profoundly influence N fluxes. For grazed pastures, plants may be exposed to urine patches that have received the equivalent of up to 1000 kg N ha -1 . We aimed to determine the growth and N fluxes in three potential trees that may be used in silvopastoral systems: L. scoparium, K. robusta and P. radiata. Plants were grown in a greenhouse lysimeter experiment, with controlled irrigation and temperature and exposed to N at rates of 200 kg ha -1 equiv. for 15 weeks, followed by the addition of 800 kg ha -1 N equiv, to simulate a urine patch. Urea produced a positive growth response of all plant species. Treatments containing L. scoparium and K. robusta leached lower amounts of nitrate (NO 3 - ) (2 kg ha -1 NO 3 - ) compared to P. radiata (53 kg ha -1 ). Measurements of N 2 O over 20 days after the application of 800 kg N ha -1 indicated an inhibitory effect of L. scoparium and K. robusta on denitrification, hence loss of N via N 2 O. Both L. scoparium and K. robusta demonstrated that they have potential to reduce N-losses in silvopastural systems, while producing valuable biomass. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. Evaporation from Pinus caribaea plantations on former grassland soils under maritime tropical conditions.

    NARCIS (Netherlands)

    Waterloo, M.J.; Bruijnzeel, L.A.; Vugts, H.F.; Rawaqa, T.T.

    1999-01-01

    Wet canopy and dry canopy evaporation from young and mature plantations of Pinus caribaea on former grassland soils under maritime tropical conditions in southwestern Viti Levu, Fiji, were determined using micrometeorological and hydrological techniques. Modeled annual evaporation totals (ET) of

  2. Evaporation from Pinus caribaea plantations on former grassland soils under maritime tropical conditions

    NARCIS (Netherlands)

    Waterloo, M.J.; Bruijnzeel, L.A.; Vugts, H.F.; Rawaqa, T.T.

    1999-01-01

    Wet canopy and dry canopy evaporation from young and mature plantations of Pinus caribaea on former grassland soils under maritime tropical conditions in southwestern Viti Levu, Fiji, were determined using micrometeorological and hydrological techniques. Modeled annual evaporation totals (ET) of

  3. First discovery of fossil winged seeds of Pinus L. (family Pinaceae ...

    Indian Academy of Sciences (India)

    Mahasin Ali Khan

    2017-07-04

    Jul 4, 2017 ... review the historical phytogeography and highlight the phytogeographic implication of this genus. Keywords. ... In the present paper, winged seeds of Pinus are described ..... the Himalayas and the onset and development of.

  4. Guía metodológica para obtener patrones de accidentabilidad laboral usando Data Mining

    OpenAIRE

    Cevallos Chacón, Alvaro

    2013-01-01

    El objetivo de esta investigación es desarrollar y proponer una metodología clara y objetiva que permita a las empresas de cualquier industria, investigar sobre patrones de accidentabilidad laboral usando la minería de datos como herramienta de análisis. Para tal efecto se combina los conceptos de seguridad industrial relacionados con la investigación de accidentes y toma como marco principal, la normatividad nacional vigente en materia de seguridad y salud en el trabajo. Estos conceptos son ...

  5. Medición de microdeformaciones en losas viales usando sensores de redes de Bragg en fibras ópticas

    OpenAIRE

    Vélez-Hoyos, Francisco Javier; Serpa-Imbet, Claudia Milena; Gómez-Cardona, Nelson Darío

    2010-01-01

    En este trabajo se presenta un método no invasivo para la medición de microdeformaciones en estructuras de concreto usando sensores de redes de Bragg en fibras ópticas adheridos a su superficie. Se realizan mediciones en losas viales de concreto bajo una carga estática de 10 kN, encontrándose una relación aproximada de 2 : 1 entre la deformación registrada por los sensores y los valores arrojados por una simulación computacional con el método de elementos finitos. Se propone el uso de estos s...

  6. (Vigna radiata) seeds

    African Journals Online (AJOL)

    hp

    and maximum activity was observed at 70°C. The optimal pH value of the enzyme activity was found to be 5.2. There was a ... expresses its isozymes in many plants such as soyabean .... Firstly, it can change the ionization of the enzyme.

  7. (Vigna radiata (L.) Wilczek)

    Indian Academy of Sciences (India)

    hectare with 1.02 million tonne production (average during. 2000–2003). This crop provides ... rinsed with 70% ethanol for 10–15 min and dried at room temperature overnight. .... gating generation led to differentiation. Cluster IV exhibited.

  8. Growth decline assessment in Pinus sylvestris L. and Pinus nigra Arnold. forest by using 3-PG model.

    Directory of Open Access Journals (Sweden)

    Rafael M. Navarro-Cerrillo

    2016-12-01

    Full Text Available Aim of the study: We assessed the ability of the 3-PG process-based model to accurately predict growth of Pinus sylvestris and P. nigra plantations across a range of sites, showing declining growth trends, in southern Spain. Area of study: The study area is located in “Sierra de Los Filabres” (Almería. Material and methods: The model was modified in fifteen parameters to predict diameter (DBH, cm, basal area increment (BAI, cm2 yr-1 and leaf area index (LAI, m2 m-2 in healthy trees and trees showing declining growth. We assumed that a set of specific physiological parameters (stem partitioning ratio-pFS20, maximum litterfall rate-γFx, maximum canopy conductance-gCx, specific leaf area for mature aged stands-σ1, age at which specific leaf area = ½ (σ0 + σ1, age at full canopy cover-tc, and canopy boundary layer conductance-gB included in 3-PG would be suitable for predicting growth decline related to climate conditions. The calibrated model was evaluated using dendrochronological and LAI data obtained from plots. Main results: Observed and simulated DBH showed a high correlation (R2 > 0.99 between modelled and measured values for both species. In contrast, modelled and observed BAI showed lower correlation (R2 < 0.68. Sensitivity analysis on 3-PG outputs showed that the foliage parameters - maximum litterfall rate, maximum canopy conductance, specific leaf area for mature aged stands, age at which specific leaf area, and age at full canopy cover - were important for DBH and BAI predictions under drought stress. Research highlights: Our overall results indicated that the 3-PG model could predict growth response of pine plantations to climatic stress with desirable accuracy in southern Spain by using readily available soil and climatic data with physiological parameters derived from experiments. Keywords: Hybrid process model; forest management models; growth prediction; Pinus spp, Parameterization; forest decline.

  9. Greek Pinus essential oils: larvicidal activity and repellency against Aedes albopictus (Diptera: Culicidae).

    Science.gov (United States)

    Koutsaviti, Katerina; Giatropoulos, Athanassios; Pitarokili, Danae; Papachristos, Dimitrios; Michaelakis, Antonios; Tzakou, Olga

    2015-02-01

    The needle volatiles metabolites of seven Pinus spp.: Pinus nigra (3 samples), Pinus stankewiczii, Pinus brutia, Pinus halepensis, Pinus canariensis, Pinus pinaster and Pinus strobus from Greece were determined by gas chromatography and gas chromatography-mass spectrometry. P. nigra and P. canariensis essential oils were dominated by α-pinene (24.9-28.9 % and 15 %, respectively) and germacrene D (20.3-31.9 % and 55.8 %, respectively), whereas P. brutia and P. strobus by α-pinene (20.6 % and 31.4 %, respectively) and β-pinene (31.7 % and 33.6 %, respectively). P. halepensis and P. pinaster oils were characterized by β-caryophyllene (28.5 % and 22.5 %, respectively). Finally, β-pinene (31.4 %), germacrene D (23.3 %) and α-pinene (17.5 %) were the most abundant compounds in the needle oil of P. stankewiczii. Additionally the larvicidal and repellent properties of their essential oils were evaluated against Aedes albopictus, a mosquito of great ecological and medical importance. The results of bioassays revealed that repellent abilities of the tested essential oils were more potent than their larvicidal activities. The essential oils of P. brutia, P. halepensis and P. stankewiczii presented considerable larvicidal activity (LC50 values 67.04 mgL(-1) and 70.21 mgL(-1), respectively), while the others were weak to inactive against larvae. The essential oils of P. halepensis, P. brutia, and P. stankewiczii presented a high repellent activity, even at the dose of 0.2 μL cm(-2), while in the dose of 0.4 μL cm(-2), almost all the tested EOs displayed protection against the mosquito.

  10. PRODUCCIÓN DE HOJARASCA EN UN RODAL DE Pinus cembroides Zucc. EN ARTEAGA, COAHUILA, MÉXICO Y SU RELACIÓN CON LAS VARIABLES CLIMÁTICAS

    Directory of Open Access Journals (Sweden)

    Santiago A. Reyes-Carrera

    2013-01-01

    Full Text Available La caída de hojarasca constituye la fuente principal de materia orgánica en los ecosistemas terrestres. La evaluación de la caída de hojarasca es importante para entender la productividad forestal, pero también proporciona información relevante como indicador fenológico de los efectos del cambio climático. Este estudio se llevó a cabo en un rodal joven de Pinus cembroides Zucc. en la sierra de Arteaga, Coahuila, México. La hojarasca fue colectada mensualmente usando 40 colectores (1 m2 ubicados sistemáticamente dentro de una parcela de 1 ha. La prueba no paramétrica de Kruskal-Wallis se utilizó para detectar diferencias significativas entre fechas de colecta. Las relaciones de producción de hojarasca con las variables climáticas se evaluaron mediante análisis de componentes principales y modelos de regresión. La deposición total de hojarasca fue poco más de 1,000 kg·ha-1·año-1, la cual fue dependiente de la temperatura y el viento. Las acículas y ramas constituyeron cerca del 33 y 22 % de la deposición total, respectivamente.

  11. Belowground legacies of Pinus contorta invasion and removal result in multiple mechanisms of invasional meltdown.

    Science.gov (United States)

    Dickie, Ian A; St John, Mark G; Yeates, Gregor W; Morse, Chris W; Bonner, Karen I; Orwin, Kate; Peltzer, Duane A

    2014-01-01

    Plant invasions can change soil biota and nutrients in ways that drive subsequent plant communities, particularly when co-invading with belowground mutualists such as ectomycorrhizal fungi. These effects can persist following removal of the invasive plant and, combined with effects of removal per se, influence subsequent plant communities and ecosystem functioning. We used field observations and a soil bioassay with multiple plant species to determine the belowground effects and post-removal legacy caused by invasion of the non-native tree Pinus contorta into a native plant community. Pinus facilitated ectomycorrhizal infection of the co-occurring invasive tree, Pseudotsuga menziesii, but not conspecific Pinus (which always had ectomycorrhizas) nor the native pioneer Kunzea ericoides (which never had ectomycorrhizas). Pinus also caused a major shift in soil nutrient cycling as indicated by increased bacterial dominance, NO3-N (17-fold increase) and available phosphorus (3.2-fold increase) in soils, which in turn promoted increased growth of graminoids. These results parallel field observations, where Pinus removal is associated with invasion by non-native grasses and herbs, and suggest that legacies of Pinus on soil nutrient cycling thus indirectly promote invasion of other non-native plant species. Our findings demonstrate that multi-trophic belowground legacies are an important but hitherto largely unconsidered factor in plant community reassembly following invasive plant removal. Published by Oxford University Press on behalf of the Annals of Botany Company.

  12. COMPARAÇÃO GRÁFICA ENTRE CURVAS DE ÍNDICE DE SÍTIO PARA Pinus elliottii E Pinus taeda DESENVOLVIDAS NO SUL DO BRASIL

    OpenAIRE

    Helio Tonini; César Augusto Guimarães Finger; Paulo Renato Schneider; Peter Spathelf

    2002-01-01

    Este trabalho teve como objetivos estudar o crescimento em altura dominante para Pinus elliottii e Pinus taeda, mediante comparações entre as curvas de índice de sítio construídas para algumas regiões do estado do Rio Grande do Sul e outros Estados da Federação. Essas comparações indicaram que as curvas feitas por Tonini (2000) para as regiões da Serra do Sudeste e Litoral no estado do Rio Grande do Sul, não apresentaram o mesmo desenvolvimento em relação às curvas feitas por Scolforo e Macha...

  13. Aptitud combinatoria general y especifica de líneas tropicales de maiz usando probadores

    Directory of Open Access Journals (Sweden)

    Mauro Sierra

    2000-01-01

    Full Text Available Aptitud combinatoria general y específica de líneas tropicales de maíz usando probadores. Durante el ciclo O - I 1996/97 fueron evaluados en el Campo Experimental Cotaxtla mestizos de líneas sobresalientes y provenientes de varias fuentes de germoplasma como son : a Líneas recicladas de H-513 X VS-536, b Líneas derivadas de un compuesto de amplia base genética, c Líneas élite de programa de maíz de Cotaxtla (LTs y d Líneas de CIMMYT (CMLs. Como probadores se usaron las líneas LT-154 y LT-155 progenitores del híbrido H-513 y las líneas CML247 y CML254 cuya cruza es un patrón heterótico definido por CIMMYT para el trópico. Hubo líneas con buen comportamiento per-se tanto en rendimiento como en características agronómicas y que se encuentran formando mestizos sobresalientes con uno o varios probadores. Con relación a la Aptitud combinatoria, se encontró que las líneas F31XF30-4-3-1, F41XF40-1-2-1, CABG3’-12-2-1-2-1-1, LT174 y CML15 registraron los máximos valores con el probador 2 (LT155; F4XF3-5-2-1 y CML15 con el probador 4 (CMl254. Así también, las líneas F4XF5-5-1-1, y CABG3’-12-2-1-2-1-1, LT174, CML13 y CML15 con buena ACG. Con relación a los probadores, se encontró que para el grupo de líneas Recicladas , los probadores 1(LT154 y 4 (CML254 registraron los coeficientes de regresión más altos, lo que indica que permiten identificar líneas sobresalientes. Para líneas CABG fué el probador 2 (LT155 el que registró el mejor valor y en líneas Élite , los probadores 2(LT155, y 3(CML247 identificaron mejor a las líneas sobresalientes. Para las líneas del CIMMYT el mejor valor fué para el probador 4(CML254

  14. Solucionando dificultades en el aula: una estrategia usando el aprendizaje basado en problemas

    Directory of Open Access Journals (Sweden)

    Mery Luz Valderrama Sanabria

    2017-09-01

    Full Text Available Introducción: Se utiliza el proyecto de investigación en el aula con énfasis en el Aprendizaje Basado en Problemas, el cual promueve el aprendizaje activo y significativo, permitiendo solucionar situaciones reales de conocimiento en torno a una temática específica. Implementa los principios de la investigación formativa, como herramienta para generar nuevas alternativas en la apropiación del conocimiento. Este estudio tuvo como objetivo conocer la percepción de los estudiantes del programa Regencia de Farmacia frente a la utilización del aprendizaje basado en problemas con el fin de realizar aportes al currículo. Materiales y Métodos: Estudio descriptivo y transversal realizado con una muestra no probabilística, por conveniencia, conformada por 109 estudiantes de segundo a sexto semestre. Se elaboró un cuestionario con escala tipo Likert, sometido a valoración por expertos. Resultados: En general, los estudiantes están de acuerdo con la estrategia porque ha permitido acercarse a la investigación, fortaleciendo el pensamiento crítico; generando autonomía y responsabilidad frente al aprendizaje. A medida que avanzan los semestres, le ven mayor utilidad. Sin embargo, falta claridad en el uso de la metodología y capacitación por parte de algunos docentes para desarrollarla eficazmente. Discusión: La coordinación docente es fundamental, se debe fortalecer este aspecto para dar claridad al uso de la metodología. Implementar procesos de evaluación para determinar los avances desarrollados por los estudiantes y el impacto generado. Conclusiones: Los estudiantes consideran que adquieren conocimientos y competencias que les ayudarán en la práctica profesional.  Cómo citar este artículo: Valderrama ML, Castaño GA. Solucionando dificultades en el aula: una estrategia usando el aprendizaje basado en problemas. Rev Cuid. 2017; 8(3: 1907-18. http://dx.doi.org/10.15649/cuidarte.v8i3.456

  15. FABRICAÇÃO DE CHAPAS DE PARTÍCULAS AGLOMERADAS USANDO GESSO COMO MATERIAL CIMENTANTE

    Directory of Open Access Journals (Sweden)

    Clovis Roberto Haselein

    2002-04-01

    Full Text Available Neste trabalho, foram confeccionadas chapas aglomeradas, utilizando gesso como material cimentante e papel reciclável dissociado (jornal e offset e partículas de madeira de pinus como reforços. Em todos os tratamentos, a razão madeira (ou fibras para gesso foi mantida em 0,25 (base seca e duas dosagens de água (w foram empregadas: 0,4 e 0,8, correspondendo à razão água:gesso. As chapas foram prensadas a frio em prensa de laboratório, em um processo similar à produção de chapas aglomeradas convencionais. Após condicionadas em câmara climatizada, as chapas foram testadas em flexão estática, dureza, arrancamento de parafusos, absorção d’água e inchamento em espessura. Em geral, a adição de fibras causou melhoria nas propriedades das chapas. Diferenças estatísticas significativas em relação à testemunha (gesso puro foram encontradas para resistência à flexão estática (MOR, dureza e arrancamento de parafusos em relação a alguns dos tratamentos estudados. Já a inclusão de fibras de papel reciclável, com w = 0,4, não apresentou diferenças significativas em relação à testemunha para absorção d’água e inchamento em espessura. Os melhores resultados foram encontrados com papel jornal, com um coeficiente w = 0,4.

  16. Extraction of cellulose nanofibers from Pinus oocarpa residues

    Energy Technology Data Exchange (ETDEWEB)

    Manrich, Anny; Martins, Maria Alice, E-mail: anny@daad-alumni.de [EMBRAPA Instrumentacao, Sao Carlos, SP (Brazil); Moraes, Jheyce Cristina; Pasquoloto, Camila [Universidade Federal de Sao Carlos (UFSCar), SP (Brazil)

    2016-07-01

    Full text: Pinus oocarpa, which wood is moderately hard and tough, is planted in Brazil for reforestation and employed for timber production used in constructions. The wood residues, such as shavings, bark and sawdust represent 30% to 50% of the total volume of wood production, of which the sawdust is 10%{sup 1}. Cellulose nanofibers is nanomaterials having a diameter between 5 nm and 20 nm and a length of up to hundreds of nm. To obtain nanofibers from cellulose sources, such as sisal and sugarcane bagasse, is used chemical processes, in which the lignocellulosic material initially undergoes pre-treatments to promote partial separation of the cellulose, such as mercerisation and bleaching thus disposing lignin and hemicellulose components. Sequentially, by controlled acid hydrolysis, amorphous regions of the cellulose are removed, and crystalline cellulose is isolated in the form of cellulose nanofibers. In this work, nanofibers from sawdust of Pinnus oocarpa, containing 44.8 wt% of cellulose 20.6 wt% hemicellulose and 30.0 wt% insoluble lignin were isolated by mercerisation (NaOH 5%, 80°C, 120 min), followed by bleaching (NaOH + acetic acid + NaClO{sub 2}, 80 deg C, 240min) and acid hydrolysis (60 wt% sulfuric acid, 45 °C, 40min). Nanofibers obtained were characterized by DRX and SEM-FEG. Results showed that, for used conditions, fiber acid hydrolysis was not complete, therefore a biphasic suspension was formed. Crystallinity index achieved was not much higher than that from pinus fiber itself, increasing from 62% to 65% and signs of cellulose type II were observed. SEM images showed elongated fibers, which have diameter of 15 ± 5 nm and length of hundreds of nm, what means that they have a large L/D aspect ratio. Nanofiber extraction yield was very low (1.3 wt% of initial residue). All steps of the process are being reviewed aiming at better results. 1) Morais, S. A. L.; Nascimento E. A. e D. C. Melo, 2005, R. Árvore, 29, 3, 461-470. (author)

  17. Genetic diversity and the mating system of a rare Mexican Piñon, Pinus pinceana, and a comparison with Pinus maximartinezii (Pinaceae)

    Science.gov (United States)

    F. Thomas Ledig; Miguel A. Capó-Arteaga; Paul D. Hodgskiss; Hassan Sbay; Celestino Flores-López; M. Thompson Conkle; Basilio Bermejo-Velázquez

    2001-01-01

    Weeping piñon (Pinus pinceana) has a restricted and fragmented range, trees are widely scattered within populations, and reproduction is limited. Nevertheless, genetic diversity was high; based on 27 isozyme loci in 18 enzyme systems, unbiased expected heterozygosity averaged 0.174. Differentiation also was high (FST = 0.152),...

  18. Changes in whole-tree water relations during ontogeny of Pinus flexilis and Pinus ponderosa in a high-elevation meadow.

    Science.gov (United States)

    Fischer, Dylan G; Kolb, Thomas E; DeWald, Laura E

    2002-07-01

    We measured sap flux in Pinus ponderosa Laws. and Pinus flexilis James trees in a high-elevation meadow in northern Arizona that has been invaded by conifers over the last 150 years. Sap flux and environmental data were collected from July 1 to September 1, 2000, and used to estimate leaf specific transpiration rate (El), canopy conductance (Gc) and whole-plant hydraulic conductance (Kh). Leaf area to sapwood area ratio (LA/SA) increased with increasing tree size in P. flexilis, but decreased with increasing tree size in P. ponderosa. Both Gc and Kh decreased with increasing tree size in P. flexilis, and showed no clear trends with tree size in P. ponderosa. For both species, Gc was lower in the summer dry season than in the summer rainy season, but El did not change between wet and dry summer seasons. Midday water potential (Psi(mid)) did not change across seasons for either species, whereas predawn water potential (Psi(pre)) tracked variation in soil water content across seasons. Pinus flexilis showed greater stomatal response to vapor pressure deficit (VPD) and maintained higher Psi(mid) than P. ponderosa. Both species showed greater sensitivity to VPD at high photosynthetically active radiation (PAR; > 2500 micromol m-2 s-1) than at low PAR (Pinus species, and was influenced by changes in LA/SA. Whole-tree water use and El were similar between wet and dry summer seasons, possibly because of tight stomatal control over water loss. 2002 Heron Publishing--Victoria, Canada

  19. Composition and chemical variability of Corsican Pinus halepensis cone oil.

    Science.gov (United States)

    Nam, Anne-Marie; Casanova, Joseph; Tomi, Félix; Bighelli, Ange

    2014-09-01

    The composition of the essential oil isolated from cones of Pinus halepensis grown in Corsica has been investigated by a combination of chromatographic (CC, GC) and spectroscopic (MS, 13C NMR) techniques. In total, 48 compounds that accounted for 95.5% of the whole composition have been identified. α-Pinene (47.5%) was the major component followed by myrcene (11.0%), (E)-β-caryophyllene (8.3%) and caryophyllene oxide (5.9%). Various diterpenes have been identified by 13C NMR in the fractions of CC. Fifteen oil samples isolated from cones harvested in three forests have been analyzed and two groups of similar importance have been differentiated within the 15 compositions. Oil samples of the first group contained α-pinene (mean 45.0 g/100 g, SD = 5.5) as the major component. The composition of samples of the second group was dominated by myrcene (mean 30.3 g/100g, SD = 9.0) and α-pinene (mean 24.6 g/100 g, SD = 3.1).

  20. Antioxidative properties of the essential oil from Pinus mugo.

    Science.gov (United States)

    Grassmann, Johanna; Hippeli, Susanne; Vollmann, Renate; Elstner, Erich F

    2003-12-17

    The essential oil from Pinus mugo (PMEO) was tested on its antioxidative capacity. For this purpose, several biochemical test systems were chosen (e.g., the Fenton System, the xanthine oxidase assay, or the copper-induced oxidation of low-density lipoprotein (LDL)). The results show that there is moderate or weak antioxidative activity when tested in aqueous environments, like in the Fenton system, xanthine oxidase induced superoxide radical formation, or in the HOCl driven fragmentation of 1-aminocyclopropane-1-carboxylic acid (ACC). In contrast, when tested in more lipophilic environments (e.g., the ACC-cleavage by activated neutrophils in whole blood) the PMEO exhibits good antioxidative activity. PMEO does also show good antioxidative capacity in another lipophilic test system (i.e., the copper induced oxidation of LDL). Some components of PMEO (i.e., Delta(3)-carene, camphene, alpha-pinene, (+)-limonene and terpinolene) were also tested. As the PMEO, they showed weak or no antioxidant activity in aqueous environments, but some of them were effective antioxidants regarding ACC-cleavage by activated neutrophils in whole blood or copper-induced LDL-oxidation. Terpinolene, a minor component of PMEO, exhibited remarkable protection against LDL-oxidation.

  1. A single ectomycorrhizal fungal species can enable a Pinus invasion.

    Science.gov (United States)

    Hayward, Jeremy; Horton, Thomas R; Pauchard, Aníbal; Nuñnez, Martin A

    2015-05-01

    Like all obligately ectomycorrhizal plants, pines require ectomycorrhizal fungal symbionts to complete their life cycle. Pines introduced into regions far from their native range are typically incompatible with local ectomycorrhizal fungi, and, when they invade, coinvade with fungi from their native range. While the identities and distributions of coinvasive fungal symbionts of pine invasions are poorly known, communities that have been studied are notably depauperate. However, it is not yet clear whether any number of fungal coinvaders is able to support a Pinaceae invasion, or whether very depauperate communities are unable to invade. Here, we ask whether there is evidence for a minimum species richness of fungal symbionts necessary to support a pine/ectomycorrhizal fungus coinvasion. We sampled a Pinus contorta invasion front near Coyhaique, Chile, using molecular barcoding to identify ectomycorrhizal fungi. We report that the site has a total richness of four species, and that many invasive trees appear to be supported by only a single ectomycorrhizal fungus, Suillus luteus. We conclude that a single ectomycorrhizal (ECM) fungus can suffice to enable a pine invasion.

  2. Hydraulic adjustments underlying drought resistance of Pinus halepensis.

    Science.gov (United States)

    Klein, Tamir; Cohen, Shabtai; Yakir, Dan

    2011-06-01

    Drought-induced tree mortality has increased over the last decades in forests around the globe. Our objective was to investigate under controlled conditions the hydraulic adjustments underlying the observed ability of Pinus halepensis to survive seasonal drought under semi-arid conditions. One hundred 18-month saplings were exposed in the greenhouse to 10 different drought treatments, simulating combinations of intensities (fraction of water supply relative to control) and durations (period with no water supply) for 30 weeks. Stomata closed at a leaf water potential (Ψ(l)) of -2.8 MPa, suggesting isohydric stomatal regulation. In trees under extreme drought treatments, stomatal closure reduced CO(2) uptake to -1 µmol m(-2) s(-1), indicating the development of carbon starvation. A narrow hydraulic safety margin of 0.3 MPa (from stomatal closure to 50% loss of hydraulic conductivity) was observed, indicating a strategy of maximization of CO2 uptake in trees otherwise adapted to water stress. A differential effect of drought intensity and duration was observed, and was explained by a strong dependence of the water stress effect on the ratio of transpiration to evapotranspiration T/ET and the larger partitioning to transpiration associated with larger irrigation doses. Under intense or prolonged drought, the root system became the main target for biomass accumulation, taking up to 100% of the added biomass, while the stem tissue biomass decreased, associated with up to 60% reduction in xylem volume.

  3. The Transcriptomic Responses of Pinus massoniana to Drought Stress

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    Mingfeng Du

    2018-06-01

    Full Text Available Masson pine (Pinus massoniana is a major fast-growing timber species planted in southern China, a region of seasonal drought. Using a drought-tolerance genotype of Masson pine, we conducted large-scale transcriptome sequencing using Illumina technology. This work aimed to evaluate the transcriptomic responses of Masson pine to different levels of drought stress. First, 3397, 1695 and 1550 unigenes with differential expression were identified by comparing plants subjected to light, moderate or severe drought with control plants. Second, several gene ontology (GO categories (oxidation-reduction and metabolism and Kyoto Encyclopedia of Genes and Genomes (KEGG pathways (plant hormone signal transduction and metabolic pathways were enriched, indicating that the expression levels of some genes in these enriched GO terms and pathways were altered under drought stress. Third, several transcription factors (TFs associated with circadian rhythms (HY5 and LHY, signal transduction (ERF, and defense responses (WRKY were identified, and these TFs may play key roles in adapting to drought stress. Drought also caused significant changes in the expression of certain functional genes linked to osmotic adjustment (P5CS, abscisic acid (ABA responses (NCED, PYL, PP2C and SnRK, and reactive oxygen species (ROS scavenging (GPX, GST and GSR. These transcriptomic results provide insight into the molecular mechanisms of drought stress adaptation in Masson pine.

  4. Pharmaceutical and nutraceutical effects of Pinus pinaster bark extract

    Science.gov (United States)

    Iravani, S.; Zolfaghari, B.

    2011-01-01

    In everyday life, our body generates free radicals and other reactive oxygen species which are derived either from the endogenous metabolic processes (within the body) or from external sources. Many clinical and pharmacological studies suggest that natural antioxidants can prevent oxidative damage. Among the natural antioxidant products, Pycnogenol® (French Pinus pinaster bark extract) has been received considerable attention because of its strong free radical-scavenging activity against reactive oxygen and nitrogen species. P. pinaster bark extract (PBE) contains polyphenolic compounds (these compounds consist of catechin, taxifolin, procyanidins of various chain lengths formed by catechin and epicatechin units, and phenolic acids) capable of producing diverse potentially protective effects against chronic and degenerative diseases. This herbal medication has been reported to have cardiovascular benefits, such as vasorelaxant activity, angiotensin-converting enzyme inhibiting activity, and the ability to enhance the microcirculation by increasing capillary permeability. Moreover, effects on the immune system and modulation of nitrogen monoxide metabolism have been reported. This article provides a brief overview of clinical studies describing the beneficial and health-promoting effects of PBE. PMID:22049273

  5. Ophiostomatoid fungi associated with declined Pinus pinaster stands in Spain

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    Gonzalo Álvarez

    2015-04-01

    Full Text Available Aim of study: We studied the presence of fungi and distribution patterns in relation to the health status of declining Pinus pinaster trees. Area of study: Trees in two declining stands in Central Spain were allotted to three declining classes. Material and Methods: Trees in two declining stands in Central Spain were allotted to three declining classes (healthy, declining and recently dead and 3 trees of each class were felled in each stand. Wood slides (phloem and xylem were taken at six positions along the trees and samples collected from fungal identification. Main results: A total of 21 fungal taxa were isolated and identified; eleven of these species belonged to the Ophiostomatoid group. Ophiostoma minus was the most frequently isolated fungus and was identified in 22% of the samples, mainly associated to dead and diseased trees. Research highlights: Together these results suggest a putative association of O. minor with the decline in this area, and thus we suggest paying more attention to this fungus as a potential agent of decline in P. pinaster stands.

  6. Flow cytometric and morphological analyses of Pinus pinaster somatic embryogenesis.

    Science.gov (United States)

    Marum, Liliana; Loureiro, João; Rodriguez, Eleazar; Santos, Conceição; Oliveira, M Margarida; Miguel, Célia

    2009-09-25

    An approach combining morphological profiling and flow cytometric analysis was used to assess genetic stability during the several steps of somatic embryogenesis in Pinus pinaster. Embryogenic cell lines of P. pinaster were established from immature zygotic embryos excised from seeds obtained from open-pollinated trees. During the maturation stage, phenotype of somatic embryos was characterized as being either normal or abnormal. Based upon the prevalent morphological traits, different types of abnormal embryos underwent further classification and quantification. Nuclear DNA content of maritime pine using the zygotic embryos was estimated to be 57.04 pg/2C, using propidium iodide flow cytometry. According to the same methodology, no significant differences (P< or =0.01) in DNA ploidy were detected among the most frequently observed abnormal phenotypes, embryogenic cell lines, zygotic and normal somatic embryos, and somatic embryogenesis-derived plantlets. Although the differences in DNA ploidy level do not exclude the occurrence of a low level of aneuploidy, the results obtained point to the absence of major changes in ploidy level during the somatic embryogenesis process of this economically important species. Therefore, our primary goal of true-to-typeness was assured at this level.

  7. Transcriptome analysis of Pinus massoniana Lamb. microstrobili during sexual reversal

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    Feng Xiao

    2018-04-01

    Full Text Available The normal megastrobilli and microstrobilli before and after the sexual reversal in Pinus massoniana Lamb. were studied and classified using a transcriptomic approach. In the analysis, a total of 190,023 unigenes were obtained with an average length of 595 bp. The annotated unigenes were divided into 56 functional groups and 130 metabolic pathways involved in the physiological and biochemical processes related to ribosome biogenesis, carbon metabolism, and amino acid biosynthesis. Analysis revealed 4,758 differentially expressed genes (DEGs between the mega- and microstrobili from the polycone twig. The DEGs between the mega- and microstrobili from the normal twig were 5,550. In the polycone twig, 1,188 DEGs were identified between the microstrobili and the sexually reversed megastrobili. Concerning plant hormone signal transduction pathways, the DEGs from both the normal and polycone twigs displayed distinct male or female associated expression patterns. There were 36 common hormone-related DEGs from the two types of twigs of P. massoniana. Interestingly, expression of these DEGs was up-regulated in the bisexual strobili, which underwent the sexual reversal. A portion of MADS-box genes in the bisexual strobili were up-regulated relative to expression in microstrobili.

  8. Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation.

    Science.gov (United States)

    Sajid, Arfaa; Manzoor, Qaisar; Iqbal, Munawar; Tyagi, Amit Kumar; Sarfraz, Raja Adil; Sajid, Anam

    2018-01-01

    The present study was conducted to appraise the anticancer activity of Pinus roxburghii essential oil along with chemical composition evaluation. MTT assay revealed cytotoxicity induction in colon, leukemia, multiple myeloma, pancreatic, head and neck and lung cancer cells exposed to essential oil. Cancer cell death was also observed through live/dead cell viability assay and FACS analysis. Apoptosis induced by essential oil was confirmed by cleavage of PARP and caspase-3 that suppressed the colony-forming ability of tumor cells and 50 % inhibition occurred at a dose of 25 μg/mL. Moreover, essential oil inhibited the activation of inflammatory transcription factor NF-κB and inhibited expression of NF-κB regulated gene products linked to cell survival (survivin, c-FLIP, Bcl-2, Bcl-xL, c-Myc, c-IAP2), proliferation (Cyclin D1) and metastasis (MMP-9). P. roxburghii essential oil has considerable anticancer activity and could be used as anticancer agent, which needs further investigation to identify and purify the bioactive compounds followed by in vivo studies.

  9. [Cold hardiness of Pinus ponderosa, P. banksian and P. tabulaeformis].

    Science.gov (United States)

    Gong, Yuehua; Zhou, Yongxue; Fan, Junfeng; Liu, Yingzhou; Pang, Kejia

    2006-08-01

    By the method of artificial freezing, this paper made a comparative study on the cold hardiness of Pinus ponderosa, P. banksiana and P. tabulaeformis, with their inherent mechanisms approached. The results showed that the cold hardiness of these three species was in the sequence of P. banksiana > P. tabulaeformis > P. ponderosa. P. banksiana had high bound water/free water ratio (7.0) and ABA content (164.3 microg x g(-1) FW) but low K+ (2450 microg x g(-1) DW) and soluble sugar (12.0%) , P. tabulaeformis had higher contents of ABA (95.8 microg x g(-1) FW), K+ (4538 microg x g(-1) DW) and soluble sugar (18.68%) but low bound water/free water ratio (2.58), while P. ponderosa had high soluble sugar content (18.05%) but low bound water/free water ratio (2.18) and K+ (2275 microg x g(-1) DW) and ABA (63.3 microg x g(-1) FW) contents. These differences might be the reasons resulting in the different cold hardiness of these three species. Low chlorophyll content and high carotenoid/chlorophyll ratio might also contribute to the cold hardiness of P. banksiana. Therefore, though the test species are all of cold hardiness, their inherent mechanisms may be different.

  10. Aboveground Tree Biomass for Pinus ponderosa in Northeastern California

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    Todd A. Hamilton

    2013-03-01

    Full Text Available Forest managers need accurate biomass equations to plan thinning for fuel reduction or energy production. Estimates of carbon sequestration also rely upon such equations. The current allometric equations for ponderosa pine (Pinus ponderosa commonly employed for California forests were developed elsewhere, and are often applied without consideration potential for spatial or temporal variability. Individual-tree aboveground biomass allometric equations are presented from an analysis of 79 felled trees from four separate management units at Blacks Mountain Experimental Forest: one unthinned and three separate thinned units. A simultaneous set of allometric equations for foliage, branch and bole biomass were developed as well as branch-level equations for wood and foliage. Foliage biomass relationships varied substantially between units while branch and bole biomass estimates were more stable across a range of stand conditions. Trees of a given breast height diameter and crown ratio in thinned stands had more foliage biomass, but slightly less branch biomass than those in an unthinned stand. The observed variability in biomass relationships within Blacks Mountain Experimental Forest suggests that users should consider how well the data used to develop a selected model relate to the conditions in any given application.

  11. Response of Pinus ponderosa Seedlings to Stylet-Bearing Nematodes

    Science.gov (United States)

    Viglierchio, D. R.

    1979-01-01

    Of 12 stylet-bearing nematodes used for inoculations, Pratylenchus penetrans, P. brachyurus, P. vulnus, Ditylenchus destructor, Meloidogyne incognita, M. javanica, and M. hapla reproduced on Pinus ponderosa, while Xiphinema index, Aphelenchus avenae, Paratylenehus neoamblycephalus, Tylenchulus semipenetrans, and Macroposthonia xenoplax did not. P. vulnus, P. brachyurus, P. penetrans, A. avenae, D. destructor, T. semipenetrans, and P. neoamblycephalus significantly suppressed both the shoot and root wet weights of ponderosa pine seedlings obtained from stands in five different locations. X. index significantly suppressed root wet weights, M. xenoplax siguificantly suppressed shoot wet weight, and M. incognita, M. javanica, and M. hapla suppressed neither at the inoculation levels used. Injurious nematodes tended to suppress root growth more than shoot growth. Seedlings from two locations produced greater shoot growth wet weight than did seedlings from the other three locations. The more injurious nematodes tended to cause an increase in the water content of shoots. Frequency analyses of seedling population shoot-root ratios indicated that ponderosa pine seedlings could be selected for better shoot-root ratios as well as for resistance to several pathogenic nematodes. PMID:19300659

  12. MICROBIOTA OF PINUS POLLEN AS ADJUVANT FACTOR OF ALLERGY

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    Tetiana Shevtsova

    2016-06-01

    Full Text Available Bacteria, their endotoxin and mold found on pollen can be a reason of respiratory symptoms in sensitized individuals. This question concerns an anemophilous pollen more acute. In this work quantitative by dilution plating method and qualitative microbial analysis by MALDI-TOF MS Biotyper of pollen and other plants organs of Pinus sylvestris L., P. nigra Arnold, P. mugo Turra, P. armandii Franch., P. wallichiana A.B. Jacks from Nitra, Slovakia are performed which shows quantitative and species differences in mesophilic aerobic (0.00-6.27 log cfu/g and anaerobic bacteria (0.00-3.70 log cfu/g, enterococci (0.00 log cfu/g, coliform bacteria (0.00-5.29 log cfu/g, lactobacilli (0.00-4.20 log cfu/g, microscopic fungi and yeasts (2.60-5.29 log cfu/g content. Representatives of Pseudomonas (14, Bacillus (2, Acinetobacter (1, Arthrobacter (1, Pantoea (1, Klebsiella (1, Penicillium (6, Aspergillus (4, Cladosporium (1, Debaryomyces (1 genera were revealed on pine trees. The allergenic potential of the identified association of microorganisms on pollen has been evaluated based on published data. The results may be useful for aerobiologists, allergists and microbiologists, at least at the local level.

  13. Ethnobotany and phytopharmacology of Pinus roxburghii Sargent: a plant review.

    Science.gov (United States)

    Kaushik, Pawan; Kaushik, Dhirender; Khokra, Sukhbir Lal

    2013-11-01

    Traditional medicine is a blend of information gathered over generations from various communities and cultures. Pinus roxburghii Sargent (Pinaceae) commonly known as "chir pine" is widely used in traditional and folkloric systems of medicine. The all parts of the plant are believed to possess medicinal qualities in Ayurvedic and Unani systems of medicine. In these traditional systems of medicine, the plant is used to heal many diseases, including afflictions of the eyes, ears, throat, blood, and skin. The plant parts are rich in various bioactive compounds such as α-pinene, abietic acid, quercetin and xanthone. Resin acids and flavanoid form a major portion of these bioactive compounds. This review presents examples of traditional medicinal uses for P. roxburghii, and subsequently explores the current understanding of the chemical, pharmacological, and biochemical properties of the extracts and the main active constituents found in each tissue of the plant. Clinical trial information is also included where available. Careful evaluation of these data may be helpful for scientists and researchers to discover and evaluate the specific chemical entities responsible for the traditional medicinal uses of P. roxburghii.

  14. GRINDING OF HARDENED STEELS USING OPTIMIZED COOLING RECTIFICADO DE ACEROS ENDURECIDOS USANDO REFRIGERACIÓN OPTIMIZADA

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    Manoel Cléber de Sampaio Alves

    2008-06-01

    medio. Para ello, al proceso de rectificación está intrínseco el reciclaje del fluido de corte, que se destaca por su costo. A través de la variación de la velocidad de avance en el proceso de rectificación cilíndrica externa del acero ABNT D6, racionalizando la aplicación de dos fluidos de corte y usando una muela superabrasiva de CBN (nitruro de boro cúbico con ligante vitrificado, se evaluaron los parámetros de salida fuerza tangencial de corte, rugosidad, circularidad, desgaste de la herramienta, la tensión residual y la integridad superficial a través de la microscopia electrónica de barrido (SEM de las piezas de prueba. Con el análisis del desempeño fluido, muela y velocidad de inmersión se encontró las mejores condiciones de fabricación propiciando la disminución del volumen de fluido de corte, disminución del tiempo de fabricación sin perjudicar los parámetros geométricos, dimensionales, el acabado superficial y la integridad superficial de los componentes.

  15. Antidiabetic, antioxidant and anti inflammatory properties of water and n-butanol soluble extracts from Saharian Anvillea radiata in high-fat-diet fed mice.

    Science.gov (United States)

    Kandouli, Chouaib; Cassien, Mathieu; Mercier, Anne; Delehedde, Caroline; Ricquebourg, Emilie; Stocker, Pierre; Mekaouche, Mourad; Leulmi, Zineb; Mechakra, Aicha; Thétiot-Laurent, Sophie; Culcasi, Marcel; Pietri, Sylvia

    2017-07-31

    According to Saharian traditional medicine, Anvillea radiata Coss. & Dur. (Asteraceae) has been valued for treating a variety of ailments such as gastro-intestinal, liver and pulmonary diseases, and has gained awareness for its beneficial effect on postprandial hyperglycemia. However, to best of our knowledge, no detailed study of the antidiabetic curative effects of this plant has been conducted yet. To determine the hypoglycemic and antidiabetic effect of dietary supplementation with Anvillea radiata extracts on high-fat-diet (HFD)-induced obesity and insulin resistance in C57BL/6J mice in relation with antioxidant, anti-inflammatory, pancreatic beta-cells and skeletal muscle protection, and digestive enzyme inhibiting properties. Six extracts (water soluble and organic) from aerial parts of the plant were analyzed phytochemically (total phenolic and flavonoid content) and screened for in vitro superoxide (by chemiluminescence) and hydroxyl radical (by electron paramagnetic resonance spin-trapping) scavenging, antioxidant (DPPH, TRAP and ORAC assays), xanthine oxidase, metal chelating, α-amylase and α-glucosidase inhibitory property, and protective effects on copper-induced lipoprotein oxidation. Then selected hydroalcoholic and aqueous extracts were assessed for toxicity in normal human lung fibroblasts and A549 cancer cells using FMCA and MTT assays. Two water-soluble extracts having the best overall properties were assessed for their (i) protective effect at 1-15µg/mL on metabolic activity of rat insulinoma-derived INS-1 cells exposed to hyperglycemic medium, and (ii) acute hypoglycemic effect on 16-weeks HFD-induced diabetic mice. Then diabetic mice were administered HFD supplemented by extracts (up to 150mg/kg/day) for 12 additional weeks using standard diet as control and the antidiabetic drug, metformin (150mg/kg), as positive control. Then the antidiabetic, anti-inflammatory and antioxidant activity of extracts were determined. Of the highly efficient

  16. Validación de la escala para manía de la Universidad Nacional de Colombia usando el análisis de Rasch

    Directory of Open Access Journals (Sweden)

    Ricardo Sánchez

    2011-03-01

    Conclusiones. En este primer estudio de la escala para manías usando el análisis de Rasch, se detectó mal ajuste y redundancia de algunos ítems. El síndrome maníaco no queda completamente evaluado por la escala. El instrumento podría mejorarse agregando síntomas depresivos.

  17. Selectivity of Pinus sylvestris extract and essential oil to estrogen-insensitive breast cancer cells Pinus sylvestris against cancer cells.

    Science.gov (United States)

    Hoai, Nguyen Thi; Duc, Ho Viet; Thao, Do Thi; Orav, Anne; Raal, Ain

    2015-10-01

    So far, the anticancer action of pine tree extracts has mainly been shown for the species distributed widely around the Asian countries. Therefore, this study was performed to examine the potential cytotoxicity of Scots pine (Pinus sylvestris L.) native also to the European region and growing widely in Estonia. The cytotoxic activity of methanol extract and essential oil of Scots pine needles was determined by sulforhodamine B assay in different human cancer cell lines. This needle extract was found to suppress the viability of several human cancer cell lines showing some selectivity to estrogen receptor negative breast cancer cells, MDA-MB-231(half maximal inhibitory concentration [IC50] 35 μg/ml) in comparison with estrogen receptor-positive breast cancer cells, MCF-7 (IC50 86 μg/ml). It is the strongest cytotoxic effect at all measured, thus far for the needles and leaves extracts derived from various pine species, and is also the first study comparing the anticancer effects of pine tree extracts on molecularly different human breast cancer cells. The essential oil showed the stronger cytotoxic effect to both negative and positive breast cancer cell lines (both IC50 29 μg/ml) than pine extract (IC50 42 and 80 μg/ml, respectively). The data from this report indicate that Scots pine needles extract and essential oil exhibits some potential as chemopreventive or chemotherapeutic agent for mammary tumors unresponsive to endocrine treatment.

  18. Paleoclimatic implications of glacial and postglacial refugia for Pinus pumila in western Beringia

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, P M; Lozhkin, A V; Solomatkina, T B; Brown, T A

    2010-02-05

    Palynological results from Julietta Lake currently provide the most direct evidence to support the existence of a glacial refugium for Pinus pumila in mountains of southwestern Beringia. Both percentages and accumulation rates indicate the evergreen shrub survived until at least {approx}19,000 14C yr B.P. in the Upper Kolyma region. Percentage data suggest numbers dwindled into the late glaciation, whereas pollen accumulation rates point towards a more rapid demise shortly after {approx}19,000 14C yr B.P. Pinus pumila did not re-establish in any great numbers until {approx}8100 14C yr B.P., despite the local presence {approx}9800 14C yr B.P. of Larix dahurica, which shares similar summer temperature requirements. The postglacial thermal maximum (in Beringia {approx}11,000-9000 14C yr B.P.) provided Pinus pumila shrubs with equally harsh albeit different conditions for survival than those present during the LGM. Regional records indicate that in this time of maximum warmth Pinus pumila likely sheltered in a second, lower-elevation refugium. Paleoclimatic models and modern ecology suggest that shifts in the nature of seasonal transitions and not only seasonal extremes have played important roles in the history of Pinus pumila over the last {approx}21,000 14C yr B.P.

  19. Síntesis de oxitocina en fase sólida usando derivados de terbutoxicarbonilo y fluorenilmetoxicarbonilo

    OpenAIRE

    Calvo, Julio C; Barrera, Nubia F.; García, Josué A.; Guzman, Fanny; Espejo, Fabiola; Patarroyo, Manuel E.

    2010-01-01

    La oxitocina, péptido cíclico cuya secuencia es CYIQNCPLG, fué el primer péptido de importancia biológica que pudo ser sintetizado. En este trabajo se compara la síntesis de la oxitocina usando resina p metilbenzhidrilamina (MBHA) para la síntesis por estrategia t-Boc y resina Rink p-metilbenzhidrilamina (Rink MBHA) para la síntesis por estrategia Fmoc, con altos rendimientos. El péptido crudo se ciclizó en una disolución acuosa de dimetilsulfóxido al 10%. La caracterización se llevó a cabo p...

  20. Cytogenetic variability in pinus sylvestris L. populations experiencing anthropogenic influence

    International Nuclear Information System (INIS)

    Oudalova, A.; Geras'kin, S.; Vasiliev, D.; Dikarev, V.

    2004-01-01

    Techno-genic pollution has become one of the most significant ecological factors determining biosphere existence and development. An analysis of genetic consequences of the radiation accidents in the South Urals and Chernobyl has shown that mutation and recombination processes are considerably accelerated in plant and animal's populations experiencing techno-genic influence. This implies that there are complicated adaptation processes leading to changes in genetic structure of populations and increasing genetic load. Pinus sylvestris L. populations growing at the territory of the 'radon' Leningrad regional radioactive waste reprocessing enterprise and Sosnovy Bor town were monitored 6 years (1997-2002) by a set of cyto-genetical and morphological tests. Cytogenetic damage levels within intercalary meristem of needle as well as in root meristem of seedlings were found to significantly exceed corresponding controls. A higher radioresistance of the Scots pine seeds analyzed was demonstrated with an acute γ-radiation that also revealed a selection process directed at an enhancement of repair efficiency and resulting in a shift of mean values of radioresistance in populations towards higher values. An enlargement of variance of studied cytogenetic parameters was found in the populations experiencing techno-genic influence. This indicates, with an account of phenomenon of the enhanced radioresistance, that there are processes of cyto-genetical adaptation in the investigated regions. An analysis of the structure of ecological-genetical variability was carried out with the purpose of separating two components in the inter-population variability - the first is engaged to the genetically determined variability of biological characteristics intrinsic for this species, and the second is responsible for the variability originating from anthropogenic contamination of the natural habitat. Changes of these two types of variability were studied in dependence on time and techno

  1. Cytogenetic variability in pinus sylvestris L. populations experiencing anthropogenic influence

    Energy Technology Data Exchange (ETDEWEB)

    Oudalova, A.; Geras' kin, S.; Vasiliev, D.; Dikarev, V. [Russian Institute of Agricultural Radiology and Agroecology, Obninsk (Russian Federation)

    2004-07-01

    Techno-genic pollution has become one of the most significant ecological factors determining biosphere existence and development. An analysis of genetic consequences of the radiation accidents in the South Urals and Chernobyl has shown that mutation and recombination processes are considerably accelerated in plant and animal's populations experiencing techno-genic influence. This implies that there are complicated adaptation processes leading to changes in genetic structure of populations and increasing genetic load. Pinus sylvestris L. populations growing at the territory of the 'radon' Leningrad regional radioactive waste reprocessing enterprise and Sosnovy Bor town were monitored 6 years (1997-2002) by a set of cyto-genetical and morphological tests. Cytogenetic damage levels within intercalary meristem of needle as well as in root meristem of seedlings were found to significantly exceed corresponding controls. A higher radioresistance of the Scots pine seeds analyzed was demonstrated with an acute {gamma}-radiation that also revealed a selection process directed at an enhancement of repair efficiency and resulting in a shift of mean values of radioresistance in populations towards higher values. An enlargement of variance of studied cytogenetic parameters was found in the populations experiencing techno-genic influence. This indicates, with an account of phenomenon of the enhanced radioresistance, that there are processes of cyto-genetical adaptation in the investigated regions. An analysis of the structure of ecological-genetical variability was carried out with the purpose of separating two components in the inter-population variability - the first is engaged to the genetically determined variability of biological characteristics intrinsic for this species, and the second is responsible for the variability originating from anthropogenic contamination of the natural habitat. Changes of these two types of variability were studied in dependence on

  2. Pinus ponderosa: A checkered past obscured four species.

    Science.gov (United States)

    Willyard, Ann; Gernandt, David S; Potter, Kevin; Hipkins, Valerie; Marquardt, Paula; Mahalovich, Mary Frances; Langer, Stephen K; Telewski, Frank W; Cooper, Blake; Douglas, Connor; Finch, Kristen; Karemera, Hassani H; Lefler, Julia; Lea, Payton; Wofford, Austin

    2017-01-01

    Molecular genetic evidence can help delineate taxa in species complexes that lack diagnostic morphological characters. Pinus ponderosa (Pinaceae; subsection Ponderosae) is recognized as a problematic taxon: plastid phylogenies of exemplars were paraphyletic, and mitochondrial phylogeography suggested at least four subdivisions of P. ponderosa. These patterns have not been examined in the context of other Ponderosae species. We hypothesized that putative intraspecific subdivisions might each represent a separate taxon. We genotyped six highly variable plastid simple sequence repeats in 1903 individuals from 88 populations of P. ponderosa and related Ponderosae (P. arizonica, P. engelmannii, and P. jeffreyi). We used multilocus haplotype networks and discriminant analysis of principal components to test clustering of individuals into genetically and geographically meaningful taxonomic units. There are at least four distinct plastid clusters within P. ponderosa that roughly correspond to the geographic distribution of mitochondrial haplotypes. Some geographic regions have intermixed plastid lineages, and some mitochondrial and plastid boundaries do not coincide. Based on relative distances to other species of Ponderosae, these clusters diagnose four distinct taxa. Newly revealed geographic boundaries of four distinct taxa (P. benthamiana, P. brachyptera, P. scopulorum, and a narrowed concept of P. ponderosa) do not correspond completely with taxonomies. Further research is needed to understand their morphological and nuclear genetic makeup, but we suggest that resurrecting originally published species names would more appropriately reflect the taxonomy of this checkered classification than their current treatment as varieties of P. ponderosa. © 2017 Willyard et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons public domain license (CC0 1.0).

  3. Treatment of Cr/sup 3+/ contaminated soil by solid tea wastage I. A study of physiological processes of Vigna radiata

    International Nuclear Information System (INIS)

    Azmat, R.; Akhter, Y.; Sara Qureshi, S.; Ahmed, T.

    2010-01-01

    This study describes the option of using domestic tea waste in soil contaminated with the Cr/sup 3+/ trace metal due to industrial and mine activity, continuously discharging in the land and aquatic resources. This disposal of industrial wastage without proper treatment is responsible for the lowering of crop productivity with the accumulation of essential and non essential trace metals in the plants. On the other hand domestic waste management in soil and aquatic resources are also accountable for the reduced field productivity. This research discusses the proper domestic waste management in the agriculture land for the cultivation of crop in the contaminated soil. Vigna radiata has been selected as a crop to check the effects of Cr/sup 3+/ and its deletion in the contaminated soil. The highest yield was obtained when soil was mixed with tea wastage instead of spreaded tea wastage. Seed germination, morphology and physiology of 15 days old plant showed remarkable improvement in the plant growth including seed germination with activated tea wastage in the presence of Cr/sup 3+/ as compared to those plants which were grown in Cr/sup 3+/ contaminated soil only. Biochemical analysis of seedling showed an increase in the concentration of chlorophyll, carbohydrates, protein and amino acids, which confirms the remediation of contaminated soil through tea wastage. It was concluded that proper use of domestic waste can be helpful to increase the soil fertility and can concentrate the heavy toxic metals in it through complex formation. (author)

  4. Impact of pesticides on plant growth promotion of Vigna radiata and non-target microbes: comparison between chemical- and bio-pesticides.

    Science.gov (United States)

    Gupta, Sukriti; Gupta, Rashi; Sharma, Shilpi

    2014-08-01

    To compare the target and non-target effects of two chemical-pesticides (chlorpyrifos and endosulfan) with that of a bio-pesticide (azadirachtin), Vigna radiata (mung bean) was grown in a randomized pot experiment with recommended and higher application rates of pesticides. Colony counts enumerating specific microbial populations, viz. fungi, Pseudomonas, nitrogen-fixing bacteria, and phosphate-solubilizing microorganisms, were performed. In addition, several plant growth parameters such as root and shoot lengths were also monitored. It was observed that the pesticides exerted a suppressive effect on different microbial communities under study in the initial 30 days period. The bacterial and fungal populations in chlorpyrifos treated plants increased thereafter. Endosulfan resulted in enhancement of fungi and nitrogen-fixing bacteria, although phosphate-solubilizing microorganisms were suppressed at higher application rates. Azadirachtin, which is gaining popularity owing to its biological origin, did not result in enhancement of any microbial populations; on the other hand, it had a deleterious effect on phosphate-solubilizing bacteria. This study is the first to evaluate the non-target effects of pesticides with a comparison between chemical- and bio-pesticides, and also stresses the importance of critical investigation of bio-pesticides before their wide spread application in agriculture.

  5. Tillage and residue management effect on soil properties, crop performance and energy relations in greengram (Vigna radiata L. under maize-based cropping systems

    Directory of Open Access Journals (Sweden)

    J.R. Meena

    2015-12-01

    Full Text Available Effect of tillage and crop residue management on soil properties, crop performance, energy relations and economics in greengram (Vigna radiata L. was evaluated under four maize-based cropping systems in an Inceptisol of Delhi, India. Soil bulk density, hydraulic conductivity and aggregation at 0–15 cm layer were significantly affected both by tillage and cropping systems, while zero tillage significantly increased the soil organic carbon content. Yields of greengram were significantly higher in maize–chickpea and maize–mustard systems, more so with residue addition. When no residue was added, conventional tillage required 20% higher energy inputs than the zero tillage, while the residue addition increased the energy output in both tillage practices. Maize–wheat–greengram cropping system involved the maximum energy requirement and the cost of production. However, the largest net return was obtained from the maize–chickpea–greengram system under the conventional tillage with residue incorporation. Although zero tillage resulted in better aggregation, C content and N availability in soil, and reduced the energy inputs, cultivation of summer greengram appeared to be profitable under conventional tillage system with residue incorporation.

  6. Elevated [CO2] does not ameliorate the negative effects of elevated temperature on drought-induced mortality in Eucalyptus radiata seedlings.

    Science.gov (United States)

    Duan, Honglang; Duursma, Remko A; Huang, Guomin; Smith, Renee A; Choat, Brendan; O'Grady, Anthony P; Tissue, David T

    2014-07-01

    It has been reported that elevated temperature accelerates the time-to-mortality in plants exposed to prolonged drought, while elevated [CO(2)] acts as a mitigating factor because it can reduce stomatal conductance and thereby reduce water loss. We examined the interactive effects of elevated [CO(2)] and temperature on the inter-dependent carbon and hydraulic characteristics associated with drought-induced mortality in Eucalyptus radiata seedlings grown in two [CO(2)] (400 and 640 μL L(-1)) and two temperature (ambient and ambient +4 °C) treatments. Seedlings were exposed to two controlled drying and rewatering cycles, and then water was withheld until plants died. The extent of xylem cavitation was assessed as loss of stem hydraulic conductivity. Elevated temperature triggered more rapid mortality than ambient temperature through hydraulic failure, and was associated with larger water use, increased drought sensitivities of gas exchange traits and earlier occurrence of xylem cavitation. Elevated [CO(2)] had a negligible effect on seedling response to drought, and did not ameliorate the negative effects of elevated temperature on drought. Our findings suggest that elevated temperature and consequent higher vapour pressure deficit, but not elevated [CO(2)], may be the primary contributors to drought-induced seedling mortality under future climates. © 2013 John Wiley & Sons Ltd.

  7. Ethanol extract of Lycoris radiata induces cell death in B16F10 melanoma via p38-mediated AP-1 activation.

    Science.gov (United States)

    Son, Minsik; Kim, Aeyung; Lee, Jaewoo; Park, Chul-Hong; Heo, Jin-Chul; Lee, Hyun-Jin; Lee, Sang-Han

    2010-08-01

    Some active alkaloids isolated from Lycoris, a bulbous perennial herb, was shown to possess various anti-tumor and anti-inflammatory activities. In this study, we evaluated the in vitro apoptotic effect of ethanol extract from Lycoris radiata (LRE) and further probed the underlying molecular mechanisms of LRE effects. The survival rate of B16F10 melanoma cells exposed to LRE was decreased in a dose-dependent manner, cell growth was retarded by arresting cell cycle at G1 phase and apoptotic appearance such as caspase-3 activation as well as DNA fragmentation was observed by LRE treatment. In addition, LRE induced p38 and c-Jun phosphorylation, followed by activation of transcription factor AP-1. Pretreatment with the p38 inhibitor (SB203580) blocked LRE-induced AP-1 transcriptional activity, and curcumin, AP-1 inhibitor, dramatically inhibited LRE-induced apoptosis in B16F10 melanoma cells. Our results collectively indicate that LRE-mediated apoptosis occurs through the activation of p38 and AP-1 pathway and potentially LRE exhibits anti-cancer activity against B16F10 melanoma cells.

  8. Chronic suppression of testicular function by constant infusion of gonadotropin-releasing hormone agonist and testosterone supplementation in the bonnet monkey (Macaca radiata).

    Science.gov (United States)

    Ravindranath, N; Ramesh, V; Krishnamurthy, H N; Rao, A J; Moudgal, R N

    1992-03-01

    To study the efficacy of long-term buserelin acetate infusion to desensitize pituitary and block testicular function in adult male monkeys (Macaca radiata). Proven fertile male monkeys exhibiting normal testicular function. Each of the control (n = 5) and experimental monkeys (n = 10) received a fresh miniosmotic pump every 21 days, whereas pumps in controls delivered vehicle of experimentals released 50 micrograms buserelin acetate every 24 hours. On day 170 (renewed every 60 days) a silastic capsule containing crystalline testosterone (T) was implanted in the experimental monkeys. At the end of 3 years, treatment was stopped, and recovery of testicular function and fertility monitored. (1) Treatment resulted in marked reduction of nocturnal but not basal serum T; (2) the pituitary remained desensitized to buserelin acetate throughout the 3-year period; (3) animals were largely azoospermic with occasional oligospermia exhibited by two monkeys; and (4) withdrawal of treatment restored testicular function, with 70% of animals regaining fertility. Long-term infertility (but restorable) can be induced in male monkeys by constant infusion of buserelin acetate and T.

  9. Effects of Bidens pilosa L. var. radiata SCHERFF treated with enzyme on histamine-induced contraction of guinea pig ileum and on histamine release from mast cells.

    Science.gov (United States)

    Matsumoto, Takayuki; Horiuchi, Masako; Kamata, Katsuo; Seyama, Yoshiyuki

    2009-06-01

    The medical mechanism against type I allergies is to block the release or production of chemical mediators from mast cells or to block the H(1)-receptor signaling. We previously reported that the anti-allergic action of the dry powder from Bidens pilosa L. var. radiata SCHERFF treated with the enzyme cellulosine (eMMBP) was dependent on the inhibition of histamine release from mast cells. Here, we investigate that the effect of fractions in eMMBP on the histamine-induced contraction in guinea pig ileum and on the release of histamine in rat peritoneal mast cells. The histamine-induced contraction in guinea pig ileum is dose-dependently inhibited by ketotifen, an antagonist of H(1)-receptor. Fractions contained caffeic acid, caffeoylquinic acid and fractions contained flavonoids such as hyperin and isoquercitrin in eMMBP inhibit histamine release from mast cells, but only flavonoids such as hyperin, isoquercitrin and rutin suppress the histamine-induced contraction in guinea pig ileum. Moreover, the histamine-induced contraction was not affected by caffeic acid, however, such contraction was significantly inhibited by rutin. These results suggest that the primary antagonists of H(1)- receptor are different from the components in eMMBP that inhibit histamine release, and that these components participate in the anti-allergic activity of eMMBP.

  10. A facile biomimetic preparation of highly stabilized silver nanoparticles derived from seed extract of Vigna radiata and evaluation of their antibacterial activity

    Science.gov (United States)

    Choudhary, Manoj Kumar; Kataria, Jyoti; Cameotra, Swaranjit Singh; Singh, Jagdish

    2016-01-01

    The significant antibacterial activity of silver nanoparticles draws the major attention toward the present nanobiotechnology. Also, the use of plant material for the synthesis of metal nanoparticles is considered as a green technology. In this context, a non-toxic, eco-friendly, and cost-effective method has been developed for the synthesis of silver nanoparticles using seed extract of mung beans ( Vigna radiata). The synthesized nanoparticles have been characterized by UV-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), atomic absorption spectroscopy (AAS), and X-ray diffraction (XRD). The UV-visible spectrum showed an absorption peak at around 440 nm. The different types of phytochemicals present in the seed extract synergistically reduce the Ag metal ions, as each phytochemical is unique in terms of its structure and antioxidant function. The colloidal silver nanoparticles were observed to be highly stable, even after 5 months. XRD analysis showed that the silver nanoparticles are crystalline in nature with face-centered cubic geometry and the TEM micrographs showed spherical particles with an average size of 18 nm. Further, the antibacterial activity of silver nanoparticles was evaluated by well-diffusion method and it was observed that the biogenic silver nanoparticles have an effective antibacterial activity against Escherichia coli and Staphylococcus aureus. The outcome of this study could be useful for nanotechnology-based biomedical applications.

  11. Influence of reaction conditions and the char separation system on the production of bio-oil from radiata pine sawdust by fast pyrolysis

    Energy Technology Data Exchange (ETDEWEB)

    Park, Hyun Ju; Park, Young-Kwon; Kim, Joo Sik [Faculty of Environmental Engineering, University of Seoul, 90 Jeonnong-Dong, Dondaemun-Gu, Seoul 130-743 (Korea)

    2008-08-15

    Radiata pine sawdust was pyrolyzed in a bubbling fluidized bed equipped with a char separation system. The influence of the reaction conditions on the production of bio-oil was investigated through the establishment of mass balance, and the examination of the products' chemical and physical characteristics. The optimal reaction temperature for the production of bio-oil was between 673 and 723 K, and the yield was above 50 wt.% of the product. An optimal feed size also existed. In a particle with a size that was less than 0.3 mm, the bio-oil yield decreased due to overheating, which led to gas formation. A higher flow rate and feeding rate were found to be more effective for the production of bio-oil, but did not significantly affect it. The main compounds of bio-oil were phenolics, including cresol, guaiacol, eugenol, benzendiol and their derivatives, ketones, and aldehydes. In addition, high-quality bio-oils, which contained less than 0.005 wt.% of solid, no ash and low concentrations of alkali and alkaline earth metals, were produced due to the char removal system. (author)

  12. Echography of the Cervix and Uterus during the Proliferative and Secretory Phases of the Menstrual Cycle in Bonnet Monkeys (Macaca radiata)

    Science.gov (United States)

    Chaudhari, Uddhav K; Metkari, Siddnath M; Manjaramkar, Dhyananjay D; Sachdeva, Geetanjali; Katkam, Rajendra; Bandivdekar, Atmaram H; Mahajan, Abhishek; Thakur, Meenakshi H; Kholkute, Sanjiv D

    2014-01-01

    We undertook the present study to investigate the echographic characteristics of the uterus and cervix of female bonnet monkeys (Macaca radiata) during the proliferative and secretory phases of the menstrual cycle. The cervix was tortuous in shape and measured 2.74 ± 0.30 cm (mean ± SD) in width by 3.10 ± 0.32 cm in length. The cervical lumen contained 2 or 3 colliculi, which projected from the cervical canal. The echogenicity of cervix varied during proliferative and secretory phases. The uterus was pyriform in shape (2.46 ± 0.28 cm × 1.45 ± 0.19 cm) and consisted of serosa, myometrium, and endometrium. The endometrium generated a triple-line pattern; the outer and central lines were hyperechogenic, whereas the inner line was hypoechogenic. The endometrium was significantly thicker during the secretory phase (0.69 ± 0.12 cm) than during the proliferative phase (0.43 ± 0.15 cm). Knowledge of the echogenic changes in the female reproductive organs of bonnet monkeys during a regular menstrual cycle may facilitate understanding of other physiologic and pathophysiologic changes. PMID:24411775

  13. Exogenous Spermidine Alleviates Low Temperature Injury in Mung Bean (Vigna radiata L. Seedlings by Modulating Ascorbate-Glutathione and Glyoxalase Pathway

    Directory of Open Access Journals (Sweden)

    Kamrun Nahar

    2015-12-01

    Full Text Available The role of exogenous spermidine (Spd in alleviating low temperature (LT stress in mung bean (Vigna radiata L. cv. BARI Mung-3 seedlings has been investigated. Low temperature stress modulated the non-enzymatic and enzymatic components of ascorbate-glutathione (AsA-GSH cycle, increased H2O2 content and lipid peroxidation, which indicate oxidative damage of seedlings. Low temperature reduced the leaf relative water content (RWC and destroyed leaf chlorophyll, which inhibited seedlings growth. Exogenous pretreatment of Spd in LT-affected seedlings significantly increased the contents of non-enzymatic antioxidants of AsA-GSH cycle, which include AsA and GSH. Exogenous Spd decreased dehydroascorbate (DHA, increased AsA/DHA ratio, decreased glutathione disulfide (GSSG and increased GSH/GSSG ratio under LT stress. Activities of AsA-GSH cycle enzymes such as ascorbate peroxidase (APX, monodehydroascorbate reductase (MDHAR, dehydroascorbate reductase (DHAR and glutathione reductase (GR increased after Spd pretreatment in LT affected seedlings. Thus, the oxidative stress was reduced. Protective effects of Spd are also reflected from reduction of methylglyoxal (MG toxicity by improving glyoxalase cycle components, and by maintaining osmoregulation, water status and improved seedlings growth. The present study reveals the vital roles of AsA-GSH and glyoxalase cycle in alleviating LT injury.

  14. Antraquinona e surfactante para otimizaçao do processo Kraft com Pinus Spp.

    OpenAIRE

    Mocelin, Ezequiel Zatoni

    2013-01-01

    A madeira de Pinus é atualmente a matéria-prima mais utilizada na obtenção de fibras longas para papel, juntamente com o processo Kraft pela sua versatilidade e por produzir fibras com boa resistência. As atuais perspectivas florestais apontam para uma escassez da madeira de Pinus para os diversos fins, seja para madeira processada, seja para celulose e papel. O processo Kraft tem como desvantagem o baixo rendimento em celulose, devido à solubilização não somente da lignina como também parte ...

  15. Dendroclimatic analysis of Pinus pseudostrobus and Pinus devoniana in the municipalities of Áporo and Zitácuaro (Michoacán, Monarch Butterfly Biosphere Reserve

    Directory of Open Access Journals (Sweden)

    Jaume Marlès Magre

    2015-12-01

    Full Text Available This article presents the first study on dendroclimatology of Pinus pseudostrobus and Pinus devoniana in the state of Michoacán (Mexico, specifically in the municipalities of Áporo and Zitácuaro, both municipalities within the Monarch Butterfly Biosphere Reserve (MBBR. The sampling in Áporo, northwest of the MBBR, was held in Los Ejidos del Rincón del Soto and Arroyo Seco, in Sierra Chincúa (May 2011. In Zitácuaro, southwest of the reserve, a sampling was performed in the Ejido de San Juan de Zitácuaro, in the area of Ocotal and Palma, and Meso Sedano (June 2011. There were a total of 38 Pinus pseudostrobus and 12 Pinus devoniana sampled in both areas of the study and distributed in 28 trees in the municipality of Áporo and 22 in Zitácuaro. Two samples per tree were taken at 1.3 m height, resulting in a total of 100 tree cores. The dendrochronological series in Áporo for the species Pinus pseudostrobus were extended to 62 years (1949-2010 and for Pinus devoniana 86 years (1925-2010; and the series in Zitácuaro for Pinus pseudostrobus and Pinus devoniana were extended to 47 years (1964-2010 and 44 years (1967-2010, respectively. The ring chronologies were validated using the program COFECHA, which calculates the cross correlations between individual series of the tree-growth, five series were eliminated due to very low or negative correlations. The climate data from Zitácuaro were obtained from two weather stations located in the same municipality. And, in the case of Áporo, the data was obtained from stations located in Senguio. The growth rates related to the climate were obtained by removing the growth trend of each tree due to the age, size and other factors such as the competition, using the program ARSTAN. The following statistics were used to evaluate the quality of the residual chronologies and to determine the potential dendrochronology of species for the different populations: the average correlation between series (Rbar

  16. Growth decline assessment in Pinus sylvestris L. and Pinus nigra Arnold. forest by using 3-PG model

    Energy Technology Data Exchange (ETDEWEB)

    Navarro-Cerrillo, R.M.; Beira, J.; Suarez, J.; Xenakis, G.; Sánchez-Salguero, R.; Hernández-Clemente, R.

    2016-07-01

    Aim of the study: We assessed the ability of the 3-PG process-based model to accurately predict growth of Pinus sylvestris and P. nigra plantations across a range of sites, showing declining growth trends, in southern Spain. Area of study: The study area is located in “Sierra de Los Filabres” (Almería). Material and methods: The model was modified in fifteen parameters to predict diameter (DBH, cm), basal area increment (BAI, cm2 yr-1) and leaf area index (LAI, m2 m-2) in healthy trees and trees showing declining growth. We assumed that a set of specific physiological parameters (stem partitioning ratio-pFS20, maximum litterfall rate-γFx, maximum canopy conductance-gCx, specific leaf area for mature aged stands-σ1, age at which specific leaf area = ½ (σ0 + σ1), age at full canopy cover-tc, and canopy boundary layer conductance-gB) included in 3-PG would be suitable for predicting growth decline related to climate conditions. The calibrated model was evaluated using dendrochronological and LAI data obtained from plots. Main results: Observed and simulated DBH showed a high correlation (R2 > 0.99) between modelled and measured values for both species. In contrast, modelled and observed BAI showed lower correlation (R2 < 0.68). Sensitivity analysis on 3-PG outputs showed that the foliage parameters - maximum litterfall rate, maximum canopy conductance, specific leaf area for mature aged stands, age at which specific leaf area, and age at full canopy cover - were important for DBH and BAI predictions under drought stress. Research highlights: Our overall results indicated that the 3-PG model could predict growth response of pine plantations to climatic stress with desirable accuracy in southern Spain by using readily available soil and climatic data with physiological parameters derived from experiments. (Author)

  17. Crescimento em altura dominante do Pinus elliottii e Pinus taeda em solos arenizados degradados no oeste do Rio Grande do Sul

    OpenAIRE

    Schneider, Paulo Renato; Elesbão, Luiz Ernesto Grilo; Schneider, Paulo Sérgio Pigato; Longhi, Régis Villanova

    2013-01-01

    A introdução de espécies florestais de rápido crescimento em áreas com solos arenizados e degradados pode ser uma alternativa tanto para a prevenção como para a minimização desse problema. O trabalho objetivou avaliar o crescimento em altura dominante do Pinus elliottii Engelm. e Pinus taeda L. em relação aos solos arenizados e degradados por ação antrópica, no oeste do estado do Rio Grande do Sul (RS). Para isso, foram selecionadas árvores dominantes em povoamentos com 29 anos de idade, em á...

  18. Crescimento em diâmetro do Pinus elliottii e Pinus taeda em áreas arenizadas e degradadas no Oeste do Rio Grande do Sul

    OpenAIRE

    Schneider, Paulo Renato; Elesbão, Luiz Ernesto Grilo; Schneider, Paulo Sérgio Pigato; Longhi, Régis Villanova

    2014-01-01

    Este trabalho foi realizado com o objetivo de estudar o desempenho do Pinus elliottii Engelm. e Pinus taeda L. em áreas arenizadas e degradadas por ação antrópica, na região da fronteira oeste do estado do Rio Grande do Sul. Em povoamentos, foram selecionadas árvores médias, que foram abatidas e seccionadas pelo método de Smalian, a fim de obter discos de madeira para a análise dendrocronológica, para a obtenção do diâmetro por idade. O crescimento em diâmetro, quando comparado entre espécies...

  19. Stem compression reversibly reduces phloem transport in Pinus sylvestris trees.

    Science.gov (United States)

    Henriksson, Nils; Tarvainen, Lasse; Lim, Hyungwoo; Tor-Ngern, Pantana; Palmroth, Sari; Oren, Ram; Marshall, John; Näsholm, Torgny

    2015-10-01

    Manipulating tree belowground carbon (C) transport enables investigation of the ecological and physiological roles of tree roots and their associated mycorrhizal fungi, as well as a range of other soil organisms and processes. Girdling remains the most reliable method for manipulating this flux and it has been used in numerous studies. However, girdling is destructive and irreversible. Belowground C transport is mediated by phloem tissue, pressurized through the high osmotic potential resulting from its high content of soluble sugars. We speculated that phloem transport may be reversibly blocked through the application of an external pressure on tree stems. Thus, we here introduce a technique based on compression of the phloem, which interrupts belowground flow of assimilates, but allows trees to recover when the external pressure is removed. Metal clamps were wrapped around the stems and tightened to achieve a pressure theoretically sufficient to collapse the phloem tissue, thereby aiming to block transport. The compression's performance was tested in two field experiments: a (13)C canopy labelling study conducted on small Scots pine (Pinus sylvestris L.) trees [2-3 m tall, 3-7 cm diameter at breast height (DBH)] and a larger study involving mature pines (∼15 m tall, 15-25 cm DBH) where stem respiration, phloem and root carbohydrate contents, and soil CO2 efflux were measured. The compression's effectiveness was demonstrated by the successful blockage of (13)C transport. Stem compression doubled stem respiration above treatment, reduced soil CO2 efflux by 34% and reduced phloem sucrose content by 50% compared with control trees. Stem respiration and soil CO2 efflux returned to normal within 3 weeks after pressure release, and (13)C labelling revealed recovery of phloem function the following year. Thus, we show that belowground phloem C transport can be reduced by compression, and we also demonstrate that trees recover after treatment, resuming C

  20. Genetic conservation and management of the Californian endemic, Torrey Pine (Pinus torreyana Parry)

    Science.gov (United States)

    Jill A. Hamilton; Jessica W. Wright; F. Thomas. Ledig

    2017-01-01

    Torrey pine (Pinus torreyana) is one of the rarest pine species in the world. Restricted to one mainland and one island population in California, Torrey pine is a species of conservation concern under threat due to low population sizes, lack of genetic variation, and environmental stochasticity. Previous research points to a lack of within population variation that is...

  1. Geographic patterns of genetic variation and population structure in Pinus aristata, Rocky Mountain bristlecone pine

    Science.gov (United States)

    Anna W. Schoettle; Betsy A. Goodrich; Valerie Hipkins; Christopher Richards; Julie Kray

    2012-01-01

    Pinus aristata Engelm., Rocky Mountain bristlecone pine, has a narrow core geographic and elevational distribution, occurs in disjunct populations, and is threatened by rapid climate change, white pine blister rust, and bark beetles. Knowledge of genetic diversity and population structure will help guide gene conservation strategies for this species. Sixteen sites...

  2. Alkanes and terpenes in wood and leaves of Pinus jeffreyi and P. sabiniana

    Science.gov (United States)

    Robert P. Adams; Jessica W. Wright

    2012-01-01

    The wood oils of Pinus jeffreyi and P. sabiniana contain considerable amounts of heptane (76.6%, 92%), on a monoterpene basis. However, when entire wood extractables is considered, the amounts drop considerably (3.4%, 36.8%) with the major portion of the wood oils being diterpene acids. The leaf oil of P. jeffreyi...

  3. Pinus ponderosa: a taxonomic review with five subspecies in the United States

    Science.gov (United States)

    Robert Z. Callaham

    2013-01-01

    Various forms of Pinus ponderosa Douglas ex C. Lawson are found from British Columbia southward and eastward through 16 states and, perhaps, into Mexico. The status of many names previously associated with this species, but excluded here, has been clarified. Accumulated evidence based on variation in morphology and xylem monoterpenes,...

  4. The variation of microfibril angle in South African grown Pinus patula ...

    African Journals Online (AJOL)

    It has been shown for some species that the microfibril angle (MFA) of the S2 layer of tracheids is strongly related to the modulus of elasticity (MOE) of wood, even more so than wood density, especially in wood formed during juvenile growth. The objectives of this study were to describe the variation in MFA in young Pinus ...

  5. DNA analysis for section identification of individual Pinus pollen grains from Belukha glacier, Altai Mountains, Russia

    International Nuclear Information System (INIS)

    Nakazawa, Fumio; Uetake, Jun; Motoyama, Hideaki; Imura, Satoshi; Kanda, Hiroshi; Suyama, Yoshihisa; Kaneko, Ryo; Takeuchi, Nozomu; Fujita, Koji

    2013-01-01

    Pollen taxon in sediment samples can be identified by analyzing pollen morphology. Identification of related species based on pollen morphology is difficult and is limited primarily to genus or family. Because pollen grains of various ages are preserved at below 0 °C in glaciers and thus are more likely to remain intact or to suffer little DNA fragmentation, genetic information from such pollen grains should enable identification of plant taxa below the genus level. However, no published studies have attempted detailed identification using DNA sequences obtained from pollen found in glaciers. As a preliminary step, this study attempted to analyze the DNA of Pinus pollen grains extracted from surface snow collected from the Belukha glacier in the Altai Mountains of Russia in the summer of 2003. A 150-bp rpoB fragment from the chloroplast genome in each Pinus pollen grain was amplified by polymerase chain reaction, and DNA products were sequenced to identify them at the section level. A total of 105 pollen grains were used for the test, and sequences were obtained from eight grains. From the sequences obtained, the pollen grains were identified as belonging to the section Quinquefoliae. Trees of the extant species Pinus sibirica in the section Quinquefoliae are currently found surrounding the glacier. The consistency of results for this section suggests that the pollen in the glacier originated from the same Pinus trees as those found in the immediate surroundings. (letter)

  6. Mortalidade em florestas de Pinus palustris causada por tempestade de raios

    Science.gov (United States)

    Kenneth W. Outcalt; Jorge Paladino Corrêa de Lima; Jose Américo de Mello Filho

    2002-01-01

    The importance of lightning as an ignition source for the fire driven Pinus palustris ecosystem is widely recognized. Lightning also impacts this system on a smaller scale by causing individual tree mortality. The objective of this study was to determine the level of mortality due to lightning activity at the Department of Energy's Savannah...

  7. The wood quality of Pinus chiapensis (Mart.) Andresen grown in the ...

    African Journals Online (AJOL)

    The wood quality of Pinus chiapensis (Mart.) Andresen grown in the Mpumalanga forest region: scientific paper. ... When present, the amounts of included resin, pieces of bark and other debris at the occluded pruning cuts, were small and of little practical significance. The wood machined without any difficulty in the wet and ...

  8. Composition of the essential oils of Pinus nigra Arnold from Turkey

    OpenAIRE

    Turkey, Composition of the essential oils of Pin

    2010-01-01

    Essential oil composition of the needles of Pinus nigra Arnold collected from different localities in Turkey was investigated by GC and GC/MS. The main components in the oils were a-pinene, b-pinene, b-caryophyllene, and germacrene D.

  9. Composition of the essential oils of Pinus nigra Arnold from Turkey

    OpenAIRE

    Turkey, Composition of the essential oils of Pin; SEZİK, Ekrem; ÜSTÜN, Osman; DEMİRCİ, Betül; BAŞER, K. Hüsnü Can

    2014-01-01

    Essential oil composition of the needles of Pinus nigra Arnold collected from different localities in Turkey was investigated by GC and GC/MS. The main components in the oils were a-pinene, b-pinene, b-caryophyllene, and germacrene D.

  10. Chloroplast microsatellites reveal population genetic diversity in red pine, Pinus resinosa Ait

    Science.gov (United States)

    Craig S. Echt; L.L. DeVerno; M. Anzidei; G.G. Vendramin

    1998-01-01

    Variation in paternally inherited chloroplast microsatellite (cpSSR) DNA was used to study population genetic structure in red pine (Pinus resinosa Ait.), a species characterized by morphological uniformity, no allozyme variation, and limited RAPD variation. Using nine cpSSR loci, a total of 23 chloroplast haplotypes and 25 cpSSR alleles were were...

  11. Climate-related genetic variation in a threatened tree species, Pinus albicaulis

    Science.gov (United States)

    Marcus V. Warwell; Ruth G. Shaw

    2017-01-01

    PREMISE OF THE STUDY: With ongoing climate change, understanding of intraspecific adaptive variation is critical for conservation and restoration of plant species. Such information is especially scarce for threatened and endangered tree species, such as Pinus albicaulis Engelm. Therefore, our principal aims were to assess adaptive variation and characterize its...

  12. Inheritance of the bark reaction resistance mechanism in Pinus monticola infected by Cronartium ribicola

    Science.gov (United States)

    Ray J. Hoff

    1986-01-01

    Necrotic reactions in branch or main stems of western white pine (Pinus monticola Dougl.) caused by infection by the blister rust fungus (Cronartium ribicola J. C. Fisch. ex Rabenh.) are a major mechanism of resistance. Overall, 26 percent of the seedlings eliminated the fungus via this defense system. Heritability based upon crossing family groups averaged 33 percent...

  13. Inheritance of allozyme variants in bishop pine (Pinus muricata D.Don)

    Science.gov (United States)

    Constance I. Millar

    1985-01-01

    Isozyme phenotypes are described for 45 structural loci and I modifier locus in bishop pine (Pinus muricata D. Don,) and segregation data are presented for a subset of 31 polymorphic loci from 19 enzyme systems. All polymorphic loci had alleles that segregated within single-focus Mendelian expectations, although one pair of alleles at each of three...

  14. Influence of ammonia and ozone on growth and drought sensitivity of Pinus sylvestris

    NARCIS (Netherlands)

    Dueck, Th.A.; Zuin, A.; Elderson, J.

    1998-01-01

    Four-year-old Pinus sylvestris trees were exposed to ammonia (16, 55, 110 ppb for 24 h d-1)and ozone (0, 45 and 68 ppb, 9 h d-1) in a factorial design in open-top chambers for 15 months. Treatment effects on tree growth and architecture were assessed during two growing seasons; effects on

  15. CO2 AND O3 ALTER PHOTOSYNTHESIS AND WATER VAPOR EXCHANGE FOR PINUS PONDEROSA NEEDLES

    Science.gov (United States)

    1. Effects of CO2 and O3 were determined for a key component of ecosystem carbon and water cycling: needle gas exchange (photosynthesis, conductance, transpiration and water use efficiency). The measurements were made on Pinus ponderosa seedlings grown in outdoor, sunlit, mesoc...

  16. Soil enzyme activities in Pinus tabuliformis (Carriere) plantations in northern China

    Science.gov (United States)

    Weiwei Wang; Deborah Page-Dumroese; Ruiheng Lv; Chen Xiao; Guolei Li; Yong Liu

    2016-01-01

    Changes in forest stand structure may alter the activity of invertase, urease, catalase and phenol oxidase after thinning Pinus tabuliformis (Carriére) plantations in Yanqing County of Beijing, China. We examined changes in these soil enzymes as influenced by time since thinning (24, 32, and 40 years since thinning) for 3 seasons (spring, summer and autumn)...

  17. Fungal endophytes in woody roots of Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa)

    Science.gov (United States)

    J. A. Hoff; Ned B. Klopfenstein; Geral I. McDonald; Jonalea R. Tonn; Mee-Sook Kim; Paul J. Zambino; Paul F. Hessburg; J. D. Rodgers; T. L. Peever; L. M. Carris

    2004-01-01

    The fungal community inhabiting large woody roots of healthy conifers has not been well documented. To provide more information about such communities, a survey was conducted using increment cores from the woody roots of symptomless Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa) growing in dry forests...

  18. Patterns of resistance to Cronartium ribicola in Pinus aristata, Rocky Mountain bristlecone pine

    Science.gov (United States)

    A. W. Schoettle; R. A. Sniezko; A. Kegley; R. Danchok; K. S. Burns

    2012-01-01

    The core distribution of Rocky Mountain bristlecone pine, Pinus aristata Engelm., extends from central Colorado into northern New Mexico, with a disjunct population on the San Francisco Peaks in northern Arizona. Populations are primarily at high elevations and often define the alpine treeline; however, the species can also be found in open mixed conifer stands with...

  19. WATER-USE ALONG A HYDROLOGICAL GRADIENT IN CENTRAL FLORIDA: A TALE OF TWO PINUS SPECIES

    Science.gov (United States)

    Although central Florida is relatively flat, the distribution of species on the landscape is controlled by subtle changes in elevation. Along a four-meter elevation gradient, xeric sandhill vegetation dominated by Pinus palustris (Longleaf pine) gives way to mesic pine flatwoods...

  20. Biomass production in an age series of Pinus patula plantation in Tamil Nadu

    Energy Technology Data Exchange (ETDEWEB)

    Sharma, S C; Srivastava, V K

    1984-09-01

    Distribution of organic matter in different tree components of 3, 5, 9, 11 and 13 years old plantations of Pinus patula has been discussed. The total biomass ranged from 7 tonnes (3 years) to 194 tonnes (9 years) per ha with 82 to 87% being contributed by the above ground parts and 13 to 18% by root.

  1. Nuclear genetic variation across the range of ponderosa pine (Pinus ponderosa): Phylogeographic, taxonomic and conservation implications

    Science.gov (United States)

    Kevin M. Potter; Valerie D. Hipkins; Mary F. Mahalovich; Robert E. Means

    2015-01-01

    Ponderosa pine (Pinus ponderosa) is among the most broadly distributed conifer species of western North America, where it possesses considerable ecological, esthetic, and commercial value. It exhibits complicated patterns of morphological and genetic variation, suggesting that it may be in the process of differentiating into distinct regional...

  2. Forest floor depth mediates understory vigor in xeric Pinus palustris ecosystems

    Science.gov (United States)

    J. Kevin Hiers; Joseph J. O' Brien; Rodney E. Will; Robert J. Mitchell

    2007-01-01

    Longleaf pine (Pinus palustris) woodlands and savannas are among the most frequently burned ecosystems in the world with fire return intervals of 1–10 years. This fire regime has maintained high levels of biodiversity in terms of both species richness and endemism. Land use changes have reduced the area of this ecosystem by .95%, and inadequate fire...

  3. Using flotation in ethanol to separate filled and empty seeds of Pinus ...

    African Journals Online (AJOL)

    In this study, the separation of filled and empty seeds of Crimean pine [Pinus nigra Arn. ssp. pallasiana (Lamb.) Holmboe] by flotation in ethanol and the effect of this treatment on seed germination were investigated. Flotation tests in 96% ethanol by the density method and then germination tests were made on the seeds ...

  4. Interrelationships among light, photosynthesis and nitrogen in the crown of mature Pinus contorta ssp. latifolia

    Science.gov (United States)

    A. W. Schoettle; W. K. Smith

    1999-01-01

    Scaling leaf-level measurements to estimate carbon gain of entire leaf crowns or canopies requires an understanding of the distribution of photosynthetic capacity and corresponding light microenvironments within a crown. We have compared changes in the photosynthetic light response and nitrogen (N) content (per unit leaf area) of Pinus contorta Dougl. ssp. latifolia...

  5. Effects of air pollution on morphological and anatomical characteristics of Pinus Eldarica Wood

    Science.gov (United States)

    Vahidreza Safdari; Moinuddin Ahmed; Margaret S. Devall; Vilma Bayramzadeh

    2012-01-01

    Air pollution, including automobile exhaust pollution, can affect anatomical and morphological characteristics of wood. In order to evaluate this subject, the Pinus eldarica trees of Chitgar Park in Tehran, which extends from a crowded highway in the south (polluted site) to the semi polluted midsection and to Alborz Mountain in the north (unpolluted...

  6. Fusarium spp. and Pinus strobus seedlings: root disease pathogens and taxa associated with seed

    Science.gov (United States)

    C. M. Ocamb; J. Juzwik; F. B. Martin

    2002-01-01

    Eastern white pine (Pinus strobus L .) seeds were sown in soil infested wlth Fusarium proliferatum, root necrosis developed on seedling roots, and F. proliferatum as reisolated from symptomatic roots; thus, demonstrating that F. proliferatum is pathogenic to eastern white pine seedling. Soils...

  7. Simulatd Nitrogen Cycling Response to Elevated CO2 in Pinus taeda and Mixed Dediduous Forests

    Science.gov (United States)

    D.W. Johnson

    1999-01-01

    Interactions between elevated CO2 and N cycling were explored with a nutrient cycling model (NuCM, Johnson et al. 1993, 1995) for a Pinus tuedu L. site at Duke University North Carolina, and a mixed deciduous site at Walker Branch, Tennessee. The simulations tested whether N limitation would prevent growth increases in response to elevated CO...

  8. A Comparison of Fire Intensity levels for stand replacement of table mountain pine (Pinus pungens Lamb.)

    Science.gov (United States)

    Thomas A. Waldrop; Patrick H. Brose

    1999-01-01

    Stand-replacement prescribed fire has been recommended to regenerate stands of table mountain pine (Pinus pungens Lamb.) in the Southern Appalachian Mountains because the species has serotinous cones and is shade intolerant. A 350 ha prescribed fire in northeast Georgia provided an opportunity to observe overstory mortality and regeneration of table...

  9. Effects of temperature on Pinus patula seedlings growing in pots in a ...

    African Journals Online (AJOL)

    The re-establishment of Pinus patula seedlings into sites with high harvesting residue (slash) loads can negatively affect the survival of these plants. Field trials have examined the role that insect pests and fungal diseases play in causing this phenomenon. Research has also indicated that temperatures at ground level tend ...

  10. Results from four Pinus patula water planting trials in the summer ...

    African Journals Online (AJOL)

    Planting with water is used by some forestry companies in South Africa to reduce post-planting water stress. Four trials were implemented to test the response in survival of Pinus patula to water applied at planting. Two trials each were situated in the KwaZulu-Natal Midlands and Mpumalanga escarpment. The first trial at ...

  11. Growth and physiological responses to varied environments among populations of Pinus ponderosa

    Science.gov (United States)

    Jianwei Zhang; Bert M. Cregg

    2005-01-01

    We investigated population responses in physiology, morphology, and growth of mature Pinus ponderosa trees to an environmental gradient across Nebraska, USA. Ten populations from western Nebraska and eastern Wyoming were grown in three 26-year-old provenance tests from the warmest and wettest site in the east (Plattsmouth) to the intermediate site in...

  12. Adaptation des semis de Pin pignon ( Pinus pinea L.) au stress ...

    African Journals Online (AJOL)

    Adaptation des semis de Pin pignon ( Pinus pinea L.) au stress combiné hydrique et thermique par des marqueurs biochimiques. ... Objectif: L'objectif de ce travail consiste à analyser les effets d'un stress hydrique et ... from 32 Countries:.

  13. Comparative genetic responses to climate in the varieties of Pinus ponderosa and Pseudotsuga menziesii: reforestation

    Science.gov (United States)

    Gerald E. Rehfeldt; Barry C. Jaquish; Cuauhtemoc Saenz-Romero; Dennis G. Joyce; Laura P. Leites; J. Bradley St Clair; Javier Lopez-Upton

    2014-01-01

    Impacts of climate change on the climatic niche of the sub-specific varieties of Pinus ponderosa and Pseudotsuga menziesii and on the adaptedness of their populations are considered from the viewpoint of reforestation. In using climate projections from an ensemble of 17 general circulation models targeting the decade surrounding 2060, our analyses suggest that a...

  14. EFFECTS OF CARBON DIOXIDE AND OZONE ON GROWTH AND BIOMASS ALLOCATION IN PINUS PONDEROSA

    Science.gov (United States)

    The future productivity of forests will be affected by combinations of elevated atmospheric CO2 and O3. Because productivity of forests will, in part, be determined by growth of young trees, we evaluated shoot growth and biomass responses of Pinus ponderosa seedlings exposed to ...

  15. CARBON ISOTOPE DISCRIMINATION AND GROWTH RESPONSE OF OLD PINUS PONDEROSA TREES TO STAND DENSITY REDUCTIONS

    Science.gov (United States)

    Stand density reductions have been proposed as a method by which old-growth ponderosa pine (Pinus ponderosa) forests of North America can be converted back to pre-1900 conditions, thereby reducing the danger of catastrophic forest fires and insect attacks while increasing product...

  16. Reproduction ecology of Pinus halepensis : a monoecious, wind-pollinated and partially serotinous Mediterranean pine tree

    NARCIS (Netherlands)

    Goubitz, Shirrinka

    2001-01-01

    Fire is an important factor in the evolution and ecology of Mediterranean plant species. The fire frequency has increased in the 20st century. Pines are the most important tree species in the area. Pinus halepensis is the only natural pine in parts of the east Mediterranean basin, such as Israel and

  17. The genetics of shortleaf pine (Pinus echinata mill.) with implications for restoration and management

    Science.gov (United States)

    John F. Stewart; Rodney E. Will; Barbara S. Crane; C. Dana Nelson

    2016-01-01

    Shortleaf pine (Pinus echinata Mill.) is an important commercial timber resource and forest ecosystem component in the southeastern USA. The species occurs in mainly drier sites as an early- to mid-successional species, is fireadapted, and it plays an important role in the fire ecology of the region. However, shortleaf pine genetics are not well-studied, especially in...

  18. Water availability and genetic effects on wood properties of loblolly pine (Pinus taeda)

    Science.gov (United States)

    C. A. Gonzalez-Benecke; T. A. Martin; Alexander Clark; G. F. Peter

    2010-01-01

    We studied the effect of water availability on basal area growth and wood properties of 11-year-old loblolly pine (Pinus taeda L.) trees from contrasting Florida (FL) (a mix of half-sib families) and South Carolina coastal plain (SC) (a single, half-sib family) genetic material. Increasing soil water availability via irrigation increased average wholecore specific...

  19. Effects of intermediate-severity disturbance on composition and structure in mixed Pinus-hardwood stands

    Science.gov (United States)

    Benjamin Trammell; Justin Hart; Callie Schweitzer; Daniel C. Dey; Michael Steinberg

    2017-01-01

    Increasingly, forest managers intend to create or maintain mixed Pinus-hardwood stands. This stand assemblage may be driven by a variety of objectives but is often motivated by the desire to enhance native forest diversity and promote resilience to perturbations. Documenting the effects of natural disturbances on species composition and stand...

  20. Assessing the gene content of the megagenome: sugar pine (Pinus lambertiana)

    Science.gov (United States)

    Daniel Gonzalez-Ibeas; Pedro J. Martinez-Garcia; Randi A. Famula; Annette Deflino-Mix; Kristian A. Stevens; Carol A. Loopstra; Charles H. Landley; David B. Neale; Jill L. Wegryzn

    2016-01-01

    Sugar pine (Pinus lambertiana Douglas) is within the subgenus Strobus with an estimated genome size of 31 Gbp. Transcriptomic resources are of particular interest in conifers due to the challenges presented in their megagenomes for gene identification. In this study, we present the first comprehensive survey of the P. lambertiana...

  1. Cogongrass ( Imperata cylindrica ) affects above- and belowground processes in commercial loblolly pine ( Pinus taeda ) stands

    Science.gov (United States)

    Adam N. Trautwig; Lori G. Eckhardt; Nancy J. Loewenstein; Jason D. Hoeksema; Emily A. Carter; Ryan L. Nadel

    2017-01-01

    Cogongrass (Imperata cylindrica), an invasive grass species native to Asia, has been shown to reduce tree vigor in loblolly pine (Pinus taeda) plantations, which comprise more than 50% of growing stock in commercial forests of the United States. I. cylindrica produces exudates with possible allelopathic effects that may influence abundance of P. taeda symbionts, such...

  2. Assessing longleaf pine (Pinus palustris) restoration after southern pine beetle kill using a compact experimental design

    Science.gov (United States)

    J.-P. Berrill; C.M. Dagley

    2010-01-01

    A compact experimental design and analysis is presented of longleaf pine (Pinus palustris) survival and growth in a restoration project in the Piedmont region of Georgia, USA. Longleaf pine seedlings were planted after salvage logging and broadcast burning in areas of catastrophic southern pine beetle (Dendroctonus frontalis) attacks on even-aged mixed pine-hardwood...

  3. Prospects for the biological control of invasive Pinus species (Pinaceae) in South Africa

    CSIR Research Space (South Africa)

    Hoffmann, JH

    2011-09-01

    Full Text Available of Swiss stone pine (Pinus cembra) not attacked by the specialized pine cone weevil, Pissodes validirostris? A case of host selection vs. host suitability. Entomologia Experimentalis et Applicata 99: 157?163. DWAF. 2004. Key issue paper for a policy...

  4. Classification of Pinus patula, P. tecunumanii, P. oocarpa, P. caribaea var. hondurensis, and Related Taxonomic Entities

    Science.gov (United States)

    A.E. Squillace; Jesse P. Perry

    1992-01-01

    Stem xylem terpenes of 75 pine populations were studied to determine relationships among taxonomic entities. Typical Pinus patula populations occurring in areas north and west of Oaxaca, Mexico, had very high proportions of 3-phellandrene and low proportions of other constituents. Terpene compositions of populations of variety longipeduncalatain...

  5. Nursery response of container Pinus palustris seedlings to nitrogen supply and subsequent effects on outplanting performance

    Science.gov (United States)

    D. Paul Jackson; R. Kasten Dumroese; James P. Barnett

    2012-01-01

    Container longleaf pine (Pinus palustris) seedlings often survive and grow better after outplanting than bareroot seedlings. Because of this, most longleaf pine are now produced in containers. Little is known about nursery fertilization effects on the quality of container longleaf pine seedlings and how that influences outplanting performance. We compared various...

  6. Biomass and nutrients of Pinus massoniana plantations in southern China: simulations for different management practices.

    Science.gov (United States)

    Huixia Yang; Silong Wang; Jianwei Zhang; Bing Fan; Weidong Zhang

    2011-01-01

    We measured the dynamics of both biomass and nutrient pools on 7-, 17-, 31- and 51-year-old Pinus massoniana plantations in southern China. Using a chronosequence approach, we found that biomass of each component increased with aging while its proportion decreased except stem-wood. Nutrient pools varied with biomass pools except for foliage. For all harvest intensities...

  7. Análisis de la biodiversidad genética del algodón peruano usando marcadores moleculares: Avances en el 2004

    OpenAIRE

    Olórtegui, José; Espinoza, Marco; Espinoza, José; Montoya, Ysabel

    2005-01-01

    Tres mini preparaciones de extracción de ADN de algodón fueron comparadas en términos de calidad y rendimiento. El método de extracción de ADN usando CTAB fue el más eficiente (30 ug) en comparación con un kit comercial de extracción (20 ug) a partir de 100 mg de hojas cotiledonarias. La óptima calidad del ADN fue evaluada con las enzimas de restricción EcoRI y MseI. El ADN preparado será usado para iniciar el análisis de la biodiversidad genética del algodón peruano, usando marcadores mole...

  8. Estructura y diversidad genética en vacas Holstein de Antioquia usando un polimorfismo del gen bGH

    Directory of Open Access Journals (Sweden)

    Juan Rincon F.

    2013-03-01

    Full Text Available Objetivo. Determinar las frecuencias alélicas y genotípicas del polimorfismo del intrón 3 del gen bGH y estimar algunos parámetros de estructura poblacional en ganado Holstein. Materiales y métodos. El estudio se realizó con 1366 vacas Holstein en 120 hatos de 11 municipios del departamento de Antioquia. Se extrajo DNA por el método de Salting out y la genotipificación se realizó usando la técnica de PCR-RFLPs. La diversidad genética se determinó mediante la comparación de las heterocigosidades, El equilibrio de Hardy-Weinberg (HW y la diferenciación genética entre las poblaciones se realizó usando el software Arlequín 2.0 Las frecuencias alélicas y genotípicas se evaluaron mediante el paquete estadístico SAS®. Resultados. Las frecuencias genotípicas encontradas fueron 0.764 (+/+, 0.223 (+/- y 0.013 (-/- y las frecuencias alélicas 0.876 (+ y 0.124 (-. No se encontraron desviaciones del Equilibrio de Hardy Weinberg en ninguna de las subpoblaciones. La diversidad genética determinada mediante la comparación de las heterocigosidades fue relativamente baja entre poblaciones pero al interior de estas no. El valor de FST de toda la población fue de 0.0068 y significativo (p<0.05, algunos FST pareados también lo fueron, tomando valores desde 0.0 a 0.13. Los estadísticos FIT y FIS no fueron significativos. Conclusiones. El gen bGH es un candidato interesante para evaluar características de importancia económica ya que no parece haber sido sometido a selección directa, presenta una variabilidad media en las poblaciones, observándose diferenciación genética significativa entre distintos municipios, producto de los diferentes sistemas de producción y acceso a las biotecnologías.

  9. Increase in the yield of mung bean (Vigna radiata [L.] R. Wilczek) with storage of radiation-modified kappa-carrageenan

    International Nuclear Information System (INIS)

    Aurigue, F.B.; Montefalcon, D.R.V.; Dela Cruz, R.M.M.; Abad, L.V.

    2015-01-01

    Kappa-carrageenan is a sulphated polysaccharide naturally present in seaweeds. Upon gamma radiation, radiolysis produces low molecular weight κ-carrageenan with the cleavage of some of its sulphate groups. Consequently, the acidic solution may further hydrolyze κ-carrageenan. The study aims to determine the effects of application by foliar spraying of freshly prepared irradiated κ-carrageenan solution and those which has been stored for 3 months on the yield of potted mungbean (Vigna radiata) plants. Foliar application of radiation-modified κ-carrageenan solution on the Kulabo variety two weeks after sowing, at flower initiation, and at fruit formation stage resulted in 30.8% increase in pod yield and 50.2% increase in seed yield compared to plants similarly treated with NitroPlus inoculants only. Pod yield and seed yield advantage over the control plants (no inoculants and no fertilizer) were 57.2% and 83% respectively no fertilizer was used in all treatments. These result confirm the previous finding that irradiated κ-carrageenan solution sprayed every two weeks inoculants only. There was 200% yield advantage over the negative control. The increase in yield is attributed to the longer length of pod, higher number of seed per pod, heavier 100-seed weight. Interestingly, the 3 months-old solution gave better result compared to a freshly irradiated one. There was 26.5% and 26.8% difference in pod yield and seed yield, respectively. Compared with the Control plants of the Kulabo variety, those sprayed with stored κ-carrageenan solution had 105% advantage in seed yield. It was proposed that the degradation process of the oligo-κ-carrageenan continue during storage because of the acidic nature of the solution. Undoubtedly, irradiated κ-carrageenan solution has plant growth promoting activity that increase yield mung bean by spraying on the leaves at least three times before fruit development.(author)

  10. Research resources: comparative microRNA profiles in human corona radiata cells and cumulus oophorus cells detected by next-generation small RNA sequencing.

    Directory of Open Access Journals (Sweden)

    Xian-Hong Tong

    Full Text Available During folliculogenesis, cumulus cells surrounding the oocyte differentiate into corona radiata cells (CRCs and cumulus oophorus cells (COCs, which are involved in gonadal steroidogenesis and the development of germ cells. Several studies suggested that microRNAs (miRNAs play an important regulatory role at the post-transcriptional level in cumulus cells. However, comparative miRNA profiles and associated processes in human CRCs and COCs have not been reported before. In this study, miRNA profiles were obtained from CRCs and COCs using next generation sequencing in women undergoing controlled ovarian stimulation for IVF. A total of 785 and 799 annotated miRNAs were identified in CRCs and COCs, while high expression levels of six novel miRNAs were detected both in CRCs and in COCs. In addition, different expression patterns in CRCs and COCs were detected in 72 annotated miRNAs. To confirm the miRNA profile in COCs and CRCs, quantitative real-time PCR was used to validate the expression of annotated miRNAs, differentially expressed miRNAs, and novel miRNAs. The miRNAs in the let-7 family were found to be involved in the regulation of a broad range of biological processes in both cumulus cell populations, which was accompanied by a large amount of miRNA editing. Bioinformatics analysis showed that amino acid and energy metabolism were targeted significantly by miRNAs that were differentially expressed between CRCs and COCs. Our work extends the current knowledge of the regulatory role of miRNAs and their targeted pathways in folliculogenesis, and provides novel candidates for molecular biomarkers in the research of female infertility.

  11. Potential use of Vitellogenin and Zona radiata proteins as biomarkers of endocrine disruption in Peregrine falcon exposed to organochlorine compounds (DDTs, PCBs, PCDDs and PCDFs)

    Energy Technology Data Exchange (ETDEWEB)

    Jimenez, B. [CSIC, Inst. of Organic Chemistry, Madrid (Spain); Mori, G.; Concejero, M.A.; Casini, S.; Fossi, M.C. [Siena Univ. (Italy)

    2004-09-15

    Many different classes of environmental contaminants such as industrial chemicals (e.g. alkylphenols, polychlorinated biphenyls, pesticides, PAHs, polychlorinated dibenzo-p-dioxins, and dibenzofurans), ''can cause adverse effects in the reproductive functions of intact organisms or their progenies, consequent to changes in endocrine functions'' showing a so-called Endocrine disruptor activity. Avian raptor species, such as peregrine falcon (Falco peregrinus) for their peculiar position in the food web are potentially at risk in relation to the accumulation of Persistent Organic Pollutants (POPs) and toxic metals. Recent studies carried out with Peregrine falcon (Falco peregrinus) in Spain reveal a contamination with organochlorine compounds (PCDDs, PCDFs, PCBs and DDTs) which could be responsible of the decrease of successful pairs observed during the last ten years. Thus there is a need to develop sensitive diagnostic monitoring tools for the evaluation of toxicological risk and potential effects on the reproductive function and population dynamic of avian top predator species. Two markers for the detection of EDs effects in oviparous vertebrates are induction of Vitellogenin (Vtg) and Zona Radiata Proteins (ZR). Vtg, a complex phospholipoglycoprotein, is the major egg-yolk protein precursor and is normally synthesized by females in response to estradiol. ZR together with Zona Pellucida (ZP) constitutes in birds part of the eggshell. These proteins (Vtg, ZR and ZP) are normally synthesised in the liver as a response to an estrogen signal given by Estradiol. Males and sexually undifferentiated specimens also have the Vtg and ZR genes but do not express them unless exposed to estrogenic compounds. The main aim of this preliminary study was to develop methods for the detection of Vtg and ZR in plasma obtained from peregrine falcon as a specific biomarker for the evaluation of the effects of EDCs.

  12. Kajian Jumlah Biji per Lubang Tanam dan Paket Pupuk terhadap Pertumbuhan dan Hasil Kacang Hijau (Vigna radiata L. Varietas Vima-1

    Directory of Open Access Journals (Sweden)

    I PUTU PANCA DIANTARA PUTRA

    2016-05-01

    Full Text Available Study of the Number of the Amount of Beed a Hole Planting and Packages Fertilizer onGrowth and Yield of Mung Bean (Vigna radiata L. Varieties Vima-1. Mung bean (Vignaradiata L. is one of bean plant – nuts for many consumption in Indonesia. The low productwas’nt cultivation techniques that supposed to, including the amount of seed each holeplanting and fertilizer giving them. The study aims to determine the amount seed hole theplanting and fertilizer best to the growth and yield of mung bean varieties vima-1. Researchcarried out in Subak Basangbe, Perean Kangin village, Baturiti – Tabanan, in October –December, 2014. The study was designed using a factorial randomized block design. The firstfactor the amount of seed a hole planting (1, 2, 3 seed, factor two of fertilizer (organicfertilizer compost, fertilizer chemical urea + TSP + KCl, organic fertilizer liquid biourin,organic fertilizer compost and fertilizer chemical urea + TSP + KCl, organic fertilizercompost and organic fertilizer liquid biourin, fertilizer chemical urea + TSP + KCl andorganic fertilizer liquid biourin. The growth of mung bean varieties Vima-1 is mainly ofplant height and number of leaves affected very real by the number of seeds planting hole andfertilizer package. Treatment of the amount of seed planting hole and fertilizer packages tovery significant effect on yield components, especially the number of pods and number ofseeds of plants on mung bean varieties Vima-1. Treatment of the amount of seed planting holeaffects most of the components of plant growth and yield components of mung bean. Thehighest yield on three planting seeds per hole that is 3,67 t / ha dry weight oven.

  13. Monitorização de transplante cardíaco usando análises do eletrograma intracavitário

    Directory of Open Access Journals (Sweden)

    BROFMAN Paulo Roberto S.

    1997-01-01

    Full Text Available Uma série de registros do eletrograma intracavitário tem sido utilizada para monitorização não invasiva da rejeição em pacientes transplantados usando um marcapasso de dupla câmara e eletrodos endocavitários revestidos com estrutura fractal. Os sinais têm sido avaliados usando o sistema CHARM (Computerized Heart Acute Rejection Monitoring _ sistema computadorizado para monitorização da rejeição cardíaca aguda. Os relatórios obtidos com este sistema contêm curvas com parâmetros sensíveis à rejeição, que demonstram uma boa correlação com a clínica e os resultados das biópsias convencionais. A monitorização a longo prazo, usando estas análises, mostrou ser uma ferramenta valiosa no acompanhamento destes pacientes.

  14. MODELAMIENTO DE LA CINÉTICA DE BIOADSORCIÓN DE Cr (III USANDO CÁSCARA DE NARANJA

    Directory of Open Access Journals (Sweden)

    MARTHA LUCIA PINZÓN-BEDOYA

    2009-01-01

    Full Text Available En este trabajo se utilizó como material bioadsorbente cáscara naranja con el fin de remover iones cromo presentes en soluciones hipotéticas diluidas, utilizadas como modelo de aguas contaminadas con bajas concentraciones de este metal. Las condiciones de operación utilizadas fueron: relación sólido/líquido 4 g/l, tamaño de partícula ??0,425 mm, concentración inicial de disolución de Cr(III 100 mg/l, tiempo de contacto 60 h y pH constante (4 y 5. Los modelos cinéticos escogidos para identificar el mecanismo de reacción del proceso de bioadsorción usando cáscara de naranja fueron: primer orden reversible, pseudo- segundoorden, Elovich y difusión intraparticular. Los resultados indican que la ecuación de Elovich proporciona mayor exactitud en el ajuste de los datos experimentales del equilibrio a este modelo cinético. La bondad del ajuste de los datos se realizó por regresión no-lineal utilizando como criterio la minimización de la función objeto suma de los cuadrados del error, SCE, haciendo uso de la herramienta matemática MATLAB.

  15. APROXIMACIÓN AL ANÁLISIS DE LA VALORACIÓN DE LA PRUEBA USANDO MODIFIED WIGMOREAN ANALYSIS (MWA

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    Carlos del Valle Rojas

    2009-01-01

    Full Text Available El presente trabajo muestra cómo el análisis de la valoración de la prueba, usando herramientas de diagramación argumental, puede ayudar a los operadores jurídicos a evaluar la calidad de la argumentación del razonamiento jurídico. Se aplica el Modified Wigmorean Analysis (MWA a un caso penal hipotético y a un caso real, ensayando el proceso de análisis y diagramación y probando su utilidad. La conclusión reseña las ventajas y debilidades del análisis y sugiere ajustes al proceso de diagramación.The present work shows how the analysis of the valuation of the proof, using tools of plot diagramming, can help the juridical operators to evaluate the quality of the argumentation of the juridical reasoning. There is applied the Modified Wigmorean Analysis (MWA to a penal hypothetical case and to a real case, testing the process of analysis and diagramming and proving his utility. The conclusion outlines the advantages and weaknesses of the analysis and suggests adjustments to the process of diagramming.

  16. Criptosporidiose em paciente com espondilite anquilosante usando adalimumabe Cryptosporidiosis in a patient with ankylosing spondylitis treated with adalimumab

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    Fernando Augusto Chiuchetta

    2010-06-01

    Full Text Available A criptosporidiose é uma doença parasitária causada pelo protozoário Cryptosporidium sp. Observou-se um aumento no número de diagnósticos realizados nos últimos vinte anos, principalmente em pacientes que apresentam imunodeficiências como a síndrome da imunodeficiência humana adquirida e as imunodeficiências induzidas como em pacientes transplantados e nos que necessitam realizar hemodiálise frequentemente. Relata-se o caso de um jovem com espondilite anquilosante que, usando adalimumabe, apresentou diarreia devido à criptosporidiose.Cryptosporidiosis is a parasitic disease caused by a protozoan called Cryptosporidium sp. An increased number of diagnoses were made in the last 20 years, especially in patients with immunodeficiency like the acquired human immunodeficiency syndrome and induced immunodeficiency, such as in transplant patients and those who need frequent hemodialysis, has been observed. We report the case of a young patient with ankylosing spondylitis treated with adalimumab who developed chronic diarrhea secondary to cryptosporidiosis

  17. JMat - Herramienta remota de cálculo y multiusuario para el aprendizaje basado en problemas usando Matlab

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    Bladimir Bacca Cortes

    2011-01-01

    Full Text Available JMat es una herramienta de cálculo basada en JAVA y EJS (Easy Java Simulations, con un esquema cliente / servidor, soporte multi-usuario y acceso remoto a Matlab. La aplicación está orientada a brindar a los usuarios una interacción con Matlab usando tres interfaces: Consola de Comandos, donde se invocan remotamente comandos de texto compatibles con Matlab. Espacio de Trabajo y Graficación, donde se mantiene un registro automático de las variables de usuario y se grafican individualmente. Funciones de usuario y Transferencia de Archivos, donde el usuario crea sus funciones, envía y recibe datos hacia y desde el servidor. JMat requiere un acceso a Internet, un servidor remoto donde esté instalado Matlab y un cliente (Navegador WEB o aplicación. No se requiere Matlab en el cliente. JMat está siendo usada actualmente en la Universidad del Valle en los cursos de Control Automático de Procesos, Control Inteligente, Redes Neuronales Artificiales, Procesamiento de Señales y Tratamiento Digital de Imágenes como herramienta para el aprendizaje basado en problemas empleando la plataforma de eLearning de la Universidad del Valle.

  18. Volume de madeira de Pinus taeda L. em diferentes espaços vitais de crescimento. Wood volume of Pinus taeda L. at different growing spacings.

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    Rodrigo LIMA

    2015-12-01

    Full Text Available Pinus taeda L. é uma das espécies do gênero Pinus mais plantadas na região Sul do Brasil por apresentar excelente crescimento e ótima adaptação às condições climáticas e de solo. Essa espécie é utilizada em larga escala, principalmente para a produção de celulose, construção civil, laminação, produção de móveis, particulados e serraria. Objetivou-se avaliar a produção volumétrica de Pinus taeda L. em diferentes espaços vitais de crescimento (entre 1 m2 e 16 m2 por planta propiciados por nove diferentes espaçamentos entre árvores de um ensaio em cinco blocos ao acaso. O trabalho baseou-se nas medidas de altura e DAP em 25 árvores internas da parcela, aos sete anos após plantio das mudas oriundas de pomar de sementes clonal. Valores estimados de volume por hectare foram inversamente proporcionais ao aumento do espaço vital, alcançando entre 74,2 e 274,8 m3 /ha. Os incrementos médios em volume atingiram entre 10,60 e 39,25 m3 /ha/ano. Concluiu-se que, se o objetivo é a produção volumétrica mesmo com diâmetros pequenos, deve-se optar por espaços vitais menores. Quando se deseja maiores diâmetros, a opção é por espaços maiores. No presente caso, o melhor compromisso entre produção volumétrica e diâmetros grandes pode estar nos espaços vitais intermediários, entre 5 e 8 m2 para cada árvore. Pinus taeda L. is one of the most Pinus species planted in southern Brazil, because it presents excellent growth and optimum adaptation to climatic and soil condition. The species is used in large scale, mainly for cellulose production, construction, laminating, production of furniture, particulates and sawmill. It was aimed to evaluate the Pinus taeda L. volumetric production at different growth vital spaces (ranging 1 m² and 16 m² per plant provided by nine different spacings between plants, the trial was installed in a randomized blocks with five replications. The study was based on height and DBH measurements

  19. Cytoprotective effects of essential oil of Pinus halepensis L. against aspirin-induced toxicity in IEC-6 cells.

    Science.gov (United States)

    Bouzenna, Hafsia; Hfaiedh, Najla; Bouaziz, Mouhamed; Giroux-Metges, Marie-Agnès; Elfeki, Abdelfattah; Talarmin, Hélène

    2017-12-01

    Essential oils from Pinus species have been reported to have various therapeutic properties. This study was undertaken to identify the chemical composition and cytoprotective effects of the essential oil of Pinus halepensis L. against aspirin-induced damage in cells in vitro. The cytoprotection of the oil against toxicity of aspirin on the small intestine epithelial cells IEC-6 was tested. The obtained results have shown that 35 different compounds were identified. Aspirin induced a decrease in cell viability, and exhibited significant damage to their morphology and an increase in superoxide dismutase (SOD) and catalase (CAT) activities. However, the co-treatment of aspirin with the essential oil of Pinus induced a significant increase in cell viability and a decrease in SOD and CAT activities. Overall, these finding suggest that the essential oil of Pinus halepensis L. has potent cytoprotective effect against aspirin-induced toxicity in IEC-6 cells.

  20. Association of Pinus banksiana Lamb. and Populus tremuloides Michx. seedling fine roots with Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina)

    Science.gov (United States)

    Lynette R. Potvin; Dana L. Richter; Martin F. Jurgensen; R. Kasten Dumroese

    2012-01-01

    Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina, Hydanaceae), commonly regarded as a wood decay fungus, was consistently isolated from bareroot nursery Pinus banksiana Lamb. seedlings. S. brinkmannii was found in ectomycorrhizae formed by Thelephora terrestris Ehrh., ...

  1. Usage of the pruned log index for loblolly pine (Pinus taeda and slash pine (Pinus elliottii Aplicação do Índice de Tora Podada para Pinus taeda e Pinus elliottii

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    Denise Jeton Cardoso

    2010-10-01

    Full Text Available

    The assessment of the quality of clearwood produced in pruned plantations of pine is necessary, especially to set price and know the utilization potential. The pruned log index (PLI, index used in Chile and New Zealand to characterize the quality of the logs, is a function of measurable variables of each log as diameter with defects, diameter 1.3 m from the largest end and the ratio between the cylinder volume common to the entire length of the log and the scaling volume through the method Smalian. This study aims at evaluating the ITP usage for slash pine (Pinus elliottii logs at the age 24 years and loblolly pine (Pinus taeda at 19, harvested
    in the regions of Ibaiti, Paraná, and Itapeva, São Paulo. The PLI values did not exceed 2.3, which  indicates that there is little clearwood on the logs. This has been proven through the veneering results, in which the potentially clear volume in relation to the log volume ranged between 52% and 55%, but 10.3% at the maximum, was turned into clearwood veneer. The slicing procedure in the lathe proved to be suitable, since it allowed the diameter of the knotty core to be measured as soon as the knot came out. The PLI showed itself as applicable for the Brazilian conditions.

    doi: 10.4336/2010.pfb.30.62.119

  1. Climate-influenced ponderosa pine (Pinus ponderosa) seed masting trends in western Montana, USA

    OpenAIRE

    Keyes, Christopher R.; Gonzalez, Ruben Manso

    2015-01-01

    Aim of study: The aim of this study was to analyze 10-year records of ponderosa pine (Pinus ponderosa) seed production, in order to confirm synchronic seed production and to evaluate cyclical masting trends, masting depletion effect, and climate-masting relationships. Area of study: The study area was located in a P. ponderosa stand in the northern Rocky Mountains (western Montana, USA). Material and methods: The study was conducted in one stand that had been subjected to a silvicul...

  2. Site index models for calabrian pine (Pinus brutia Ten. in Thasos Island, Greece

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    Kyriaki Kitikidou

    2011-01-01

    Full Text Available A site index model for Calabrian pine (Pinus brutia Ten. in Thasos island (North Aegean sea, Greece is presented. The model was fitted and validated from 150 stem analyses, obtained from 75 fixed-area plots from five experimental sites. Four height growth equations of difference form were tested and the BAILEY and CLUTTER (1974 function was considered appropriate due to its good performance with both fitting and validation data. The results show errors lower than 5% and little bias.

  3. Modelo Bio-inspirado para el Reconocimiento de Gestos Usando Primitivas de Movimiento en Visión

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    Sandra E. Nope

    2008-10-01

    Full Text Available Resumen: Se aborda el problema del reconocimiento de gestos usando la información de movimiento con el fin de obtener un modelo bio-inspirado para, en un futuro, utilizarlo en la programación de robots mediante el paradigma del aprendizaje por imitación. En este trabajo se extraen las primitivas de movimiento a partir de imágenes consecutivas, capturadas por una cámara web estándar. Para la programación por imitación de robots se identificó, como primera fase, el reconocimiento de gestos, en el cual es necesario resolver tres aspectos principales: La representación instantánea del movimiento, la integración temporal de dicha información y, la estrategia de clasificación. Estos tres aspectos serán tratados a lo largo de este trabajo y, en contraste con otros, la extracción del movimiento y su codificación está inspirada en el procesamiento del movimiento realizado en el cerebro de macacos. El modelo obtenido fue aplicado al reconocimiento de cuatro tipos de gestos realizados con la mano por diferentes personas. El porcentaje de aciertos varió entre 91.42% y 97.14%, utilizando diferentes estrategias estándar de clasificación. Palabras clave: Reconocimiento de gestos, modelo bio-inspirado, primitivas de movimiento, codificación del movimiento, integración temporal, visión artificial

  4. Reproductive Success and Inbreeding Differ in Fragmented Populations of Pinus rzedowskii and Pinus ayacahuite var. veitchii, Two Endemic Mexican Pines under Threat

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    Paty Castilleja Sánchez

    2016-08-01

    Full Text Available Seed production, quality, germination and seedling establishment are indicators of reproductive success in conifers. Monitoring of these parameters is essential to determine the viability of populations for the purposes of conservation. We analyze cone and seed traits as indicators of reproductive success in the endangered Rzedowski´s pine (Pinus rzedowskii (Madrigal et Caballero and near-threatened veitchii pine (Pinus ayacahuite var. veitchii (Shaw in west-central Michoacán, Mexico. These traits were systematically quantified and their variation assessed using Generalized Linear Mixed Models (GLMMs. We found that the reproductive success of Rzedowski’s pine seems to be critical, presenting low seed efficiency (17.10%, germination (5.0% and seedling establishment (27.7%, with high levels of inbreeding (0.79. In contrast, veitchii pine presents moderate seed efficiency (54.9%, high germination (71.5% and seedling establishment (84%–97% and low inbreeding (0.33. Reproductive indicators differed significantly among zones and populations for each species, where fragment sizes mainly affected seed production and efficiency. This result indicates that fragmentation has played a more important role in the reproductive success of Rzedowski’s pine than in veitchii pine, perhaps by limiting pollen flow among zones and populations and producing higher levels of inbreeding and lower seed efficiency in the former species. We propose a conservation strategy for these important pine species in order to increase their long-term genetic viability.

  5. Crescimento em altura dominante do Pinus elliottii e Pinus taeda em solos arenizados degradados no oeste do Rio Grande do Sul

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    Paulo Renato Schneider

    2013-11-01

    Full Text Available A introdução de espécies florestais de rápido crescimento em áreas com solos arenizados e degradados pode ser uma alternativa tanto para a prevenção como para a minimização desse problema. O trabalho objetivou avaliar o crescimento em altura dominante do Pinus elliottii Engelm. e Pinus taeda L. em relação aos solos arenizados e degradados por ação antrópica, no oeste do estado do Rio Grande do Sul (RS. Para isso, foram selecionadas árvores dominantes em povoamentos com 29 anos de idade, em áreas com dois níveis de degradação, as quais foram abatidas e seccionadas pelo método de Smalian para a obtenção de discos de madeira para a análise dendrocronológica e determinação da altura dominante por idade, no período de 1982 a 2010. Os crescimentos em altura dominante dessas espécies, quando comparados entre si e por nível de degradação do solo, apresentaram tendências diferentes de desenvolvimento no tempo, indicando a presença de polimorfismo das curvas.

  6. Crescimento em diâmetro do Pinus elliottii e Pinus taeda em áreas arenizadas e degradadas no Oeste do Rio Grande do Sul

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    Paulo Renato Schneider

    2014-09-01

    Full Text Available Este trabalho foi realizado com o objetivo de estudar o desempenho do Pinus elliottii Engelm. e Pinus taeda L. em áreas arenizadas e degradadas por ação antrópica, na região da fronteira oeste do estado do Rio Grande do Sul. Em povoamentos, foram selecionadas árvores médias, que foram abatidas e seccionadas pelo método de Smalian, a fim de obter discos de madeira para a análise dendrocronológica, para a obtenção do diâmetro por idade. O crescimento em diâmetro, quando comparado entre espécies, apresentou tendências diferentes de níveis de desenvolvimento no tempo. O uso de variáveis dummy no modelo de crescimento permitiu definir uma equação única para estimar o diâmetro em função da idade para ambas as espécies, considerando os níveis de degradação do solo médio e baixo, como variáveis necessárias para estimar o crescimento destas espécies nestes tipos de solos arenizados e degradados.

  7. COMPARAÇÃO GRÁFICA ENTRE CURVAS DE ÍNDICE DE SÍTIO PARA Pinus elliottii E Pinus taeda DESENVOLVIDAS NO SUL DO BRASIL

    Directory of Open Access Journals (Sweden)

    Helio Tonini

    2002-04-01

    Full Text Available Este trabalho teve como objetivos estudar o crescimento em altura dominante para Pinus elliottii e Pinus taeda, mediante comparações entre as curvas de índice de sítio construídas para algumas regiões do estado do Rio Grande do Sul e outros Estados da Federação. Essas comparações indicaram que as curvas feitas por Tonini (2000 para as regiões da Serra do Sudeste e Litoral no estado do Rio Grande do Sul, não apresentaram o mesmo desenvolvimento em relação às curvas feitas por Scolforo e Machado (1988, para os estados do Paraná e Santa Catarina; Brasil (1989a, para a região de Passo Fundo RS, Marcolin (1990, para o Segundo Planalto Paranaense e Selle (1993, para a região de Cambará do Sul. No entanto, o comportamento em relação às curvas de índice de sítio feitas por Brasil (1989b, para a região de São Francisco de Paula mostraram-se semelhantes, havendo somente uma diferença de nível entre estas.

  8. Mycorrhization of containerised Pinus nigra seedlings with Suillus granulatus under open field conditions

    Energy Technology Data Exchange (ETDEWEB)

    Lazarevic, J.; Keca, N.; Martinovie, A.

    2012-07-01

    Seedling mycorrhization acts as an efficient tool for improving the quality of seedlings. In this study, the effectiveness of Suillus granulatus, originating from Pinus heldreichii forests (Montenegro), to produce containerized ectomycorrhizal seedlings of autochthonous Pinus nigra in open field conditions was investigated. Spore (106, 107, 108) and vegetative (1:16, 1:8, 1:4) inoculation on ectomycorrhizal formation and seedling growth were tested. Spore and vegetative inoculums of autochthonous Pisolithus arhizus were used in the same trial as additional control treatments. The utilization of vegetative and spore inoculums of autochthonous S. granulatus has proven to be an effective method of obtaining containerized ectomycorrhizal P. nigra seedlings under open field conditions after 11 months. S. granulatus spore inoculations resulted in well developed ectomycorrhiza, decreasing the growth of the P. nigra seedlings in the first growing season. Mycelial inoculations resulted in slightly developed S. granulatus ectomycorrhiza, which increased the growth of the seedlings. Therefore, it would be feasible to use spore inocula of S. granulatus, with 10{sup 6} spores per plant, to produce ectomycorrhizal P. nigra plants on a large scale. Controlled mycorrhizal inoculation of seedlings is not a common practice in Montenegrin and Serbian nurseries; as such, the obtained results will contribute to the enhancement of nursery production of Pinus nigra and other conifers. This also could be assumed as a starting point for many further efforts and investigations with autochthonous fungal and plant material in this region. (Author) 47 refs.

  9. Soil water balance in different densities of Pinus taeda in Southern Brazil

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    Jorge Luiz Moretti Souza

    2016-04-01

    Full Text Available This study aimed to quantify and compare water balance components over the course of a year for different Pinus taeda planting densities in an oxisol in southern Brazil. This experiment was conducted on 6-year-old trees in a clay oxisol at the Monte Alegre Farm, a property of the Klabin Company. The experimental design was a randomized block with four replicates and five treatments with different amounts of soil coverage: T100 (100% coverage - standard planting coverage; (T75, 75; T50, 50; and T25, 25%, and; T0 (without cover - clearcutting. The soil water storage and actual evapotranspiration under non-standard conditions were determined in a weekly estimated soil water balance (SWB with measured components. By the end of the year, the treatments had not reached field capacity or wilting point storage. The average value of total downward drainage was 100.2 mm, and the highest values occurred in the T75 and T100 treatments. The lowest population density (T25 had the highest actual evapotranspiration (ETr, due to the growth of the remaining Pinus taeda trees. The highest evapotranspiration occurred in September, due to the resumption of Pinus taeda growth.

  10. Antimicrobial activity of needle essential oil of Pinus peuce Griseb. (Pinaceae from Macedonian flora

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    Marija Karapandzova

    2012-03-01

    Full Text Available Chemical composition and antimicrobial activity of needle essential oil, obtained by hydrodistillation from wild Pinus peuce Griseb. (Pinaceae, growing on three different locations in R. Macedonia were investigated in period 2008/2009. Carried out GC/FID/MS analysis, one hundred and three constituents were identified belonging to the six different classes of components: monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpene hydrocarbons, oxygenated sesquiterpenes, diterpenes and other non-terpene components, representing 88.61/94.04% of the entire oil. The most abundant constituents were α-pinene (12.89/27.34%, β-pinene (6.16/13.13%, limonene + β-phellandrene (2.09/6.64% and bornyl acetate (2.92/11.67% as well as trans-(E-caryophyllene (4.63/7.13% and germacrene D (8.75/20.14%. Antimicrobial screening of Pinus peuce needle essential oil was made by hole-plate diffusion and broth dilution method against 13 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. The most sensitive bacteria against tested Pinus peuce essential oils were Streptococcus pneumonia encompassing Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Acinetobacter spp. and Streptococcus pyogenes. Minimal inhibitory concentrations (MICs of the oils ranged from 7.5- 62.5 µl/ml.

  11. Laboratory Evaluation of the Anti-stain Efficacy of Crude Wood Vinegar for Pinus densiflora

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    Sabiha Salim

    2016-12-01

    Full Text Available In Korea, Pinus densiflora is one of the most important indigenous tree species in terms of making high-value wood products. Therefore, Korean sawmills exercise extreme caution to prevent fungal discoloration such as that caused by sapstains and molds on the timber. In this study, the effectiveness of using natural crude wood vinegar to inhibit sapstains and molds, especially on Pinus densiflora (Japanese red pine was examined. Pinus densiflora wood samples were dipped in absolute and diluted wood vinegar at different concentrations (1:1, 2:1, and 3:1; deionized water to wood vinegar dilution ratio for 3 minutes and immediately air-dried. In addition, volatile wood vinegar was also used in this study to imitate the condition of wood vinegar when exposed to open air. The degree of discoloration was examined and evaluated every 2, 4, 6, and 8 weeks according to the ASTM D4445-91 Standard Method for laboratory test. Crude wood vinegar inhibited sapstains more efficiently than it inhibited molds. Wood vinegar at a 1:1 concentration was found to be the most optimum treatment for inhibiting sapstains for at least 8 weeks.

  12. Effects Of Drought Stress on Germination in Fourteen Provenances of Pinus Brutia Ten. Seeds in Turkey

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    Hakan Şevik

    2015-02-01

    Full Text Available Pinus brutia Ten., Red pine, known to be tough drought resistant pine specie, could effectively be used for afforestation of disturbed areas. It is of great interest for the afforestation in arid zones. Appropriate seed sources for the specific areas guarantees reforestation success. Away from its native areas Pinus brutia Ten. is planted for its ornamental value and timber production purposes. Selection of drought resistant provenances can very well increase the survival success. In this study, the effects of water potential on germination were studied in fourteen provenances of Pinus brutia Ten. from Turkey. Water potentials between 0 and -8 bars were obtained using polyethylene glycol 6000 (PEG-6000 solutions. Seeds were kept for 35 day at 20 ± 0.5°C. A decrease in water potential produced a marked reduction in germination percentage and germination value. As a result, significant variations between the provenances were found. It was determined that, under a -8 bar water stress, Isparta-Bucak and Mersin-Silifke, respectively corresponding to 58% and 57% of the control group, were the least water stress affected provenances.

  13. Fusarium oxysporum and F. verticillioides associated with damping-off in Pinus spp.

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    Caciara Gonzatto Maciel

    Full Text Available ABSTRACT Occurrence of Fusarium spp. is one of the problems, most limiting to growth of seedlings, in nurseries. This pathogen can be transmitted via seeds and causes damages to the seedlings during pre- and post-emergence stages. The present study aimed to identify Fusarium spp. at the species level based on morphological and molecular characteristics and to verify the pathogenicity of these isolates in seeds lots of Pinus elliottii and P. taeda. For this, we used two Fusarium isolates and five lots of Pinus spp. seeds. Morphological characterization was performed based on a key, specific to Fusarium spp. identification, whereas, molecular identification was carried out by amplification and sequencing of the regions from internal transcribed spacer (ITS and the elongation factor 1-α (tef1. The pathogenicity test was conducted through the contact of the seeds with fungal culture for 48 h, followed by sowing them in sand. The variables evaluated were emergency speed index, percentage of emergency, non-emergency seeds, symptomatic seedlings, and seedling damping-off. One isolate, F1UFSM, was identified as F. verticillioides and another isolate, F2UFSM, was identified as F. oxysporum. Both the isolates were pathogenic to the seeds of Pinus spp., causing a reduction in the percentage of emergence and seedling damping-off.

  14. Effects of Fusarium circinatum on Disease Development and Gas Exchange in the Seedlings of Pinus spp.

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    Kwan-Soo Woo

    2011-08-01

    Full Text Available Four-year-old seedlings of Pinus thunbergii, Pinus densiflora and Pinus rigida were inoculated with Fusarium circinatum isolate (FT-7, the pitch canker fungus, from P. thunbergii, to evaluate the effects of the pathogen on disease development and gas exchange rate. Needle dehydration was evident on 2 of 10 seedlings of P. thunbergii and P. rigida at 18 and 21 days after inoculation, respectively, while no symptoms were observed in P. densiflora seedlings throughout the experiment. Gas exchange stopped completely in 4 of 5 measured seedlings of P. thunbergii and 2 of 5 measured seedlings of P. rigida at 25 days after inoculation, and in the remaining 3 seedlings of P. rigida at 39 days after inoculation. Disease development in P. thunbergii seedlings was faster than that in P. rigida seedlings. By the time, the experiment was ended at 78 days after inoculation, 9 of 10 seedlings of P. rigida and 8 of 10 seedlings of P. thunbergii seedlings treated with FT-7 was almost dead, but all seedlings of P. densiflora were still healthy. We suggest that P. densiflora is resistant to F. circinatum in the current study, and gas exchange rate of the species after inoculation does not differ significantly compared to that of untreated control.

  15. Chloroplast evolution in the Pinus montezumae complex: a coalescent approach to hybridization.

    Science.gov (United States)

    Matos, J A; Schaal, B A

    2000-08-01

    This study addresses the evolutionary history of the chloroplast genomes of two closely related pine species, Pinus hartwegii Lindl. and P. montezumae Lamb (subsect. Ponderosae) using coalescent theory and some of the statistical tools that have been developed from it during the past two decades. Pinus hartwegii and P. montezumae are closely related species in the P. montezumae complex (subsect. Ponderosae) of Mexico and Central America. Pinus hartwegii is a high elevation species, whereas P. montezumae occurs at lower elevations. The two species occur on many of the same mountains throughout Mexico. A total of 350 individuals of P. hartwegii and P. montezumae were collected from Nevado de Colima (Jalisco), Cerro Potosí (Nuevo León), Iztaccihuatl/Popocatepetl (México), and Nevado de Toluca (México). The chloroplast genome of P. hartwegii and P. montezumae was mapped using eight restriction enzymes. Fifty-one different haplotypes were characterized; 38 of 160 restriction sites were polymorphic. Clades of most parsimoniously related chloroplast haplotypes are geographically localized and do not overlap in distribution, and the geographically localized clades of haplotypes include both P. hartwegii and P. montezumae. Some haplotypes in the clades occur in only one of the two species, whereas other haplotypes occur in both species. These data strongly suggest ancient and/or ongoing hybridization between P. hartwegii and P. montezumae and a shared chloroplast genome history within geographic regions of Mexico.

  16. Experimental study of Pinus densiflora Siebold et Zuccarini on Hyperlipidemia and lipid in Rats

    Directory of Open Access Journals (Sweden)

    Kim, Dae-Hyun

    2007-02-01

    Full Text Available In order to study the effects of Pinus densiflora on hyperlipidemia and lipid in rats, we divided the rats into groups(Normal group, Control group and Sample group and perfomed the experimental research. Hyperlipidemia and lipid in rats were induced by high fat diets for 8weeks. The sample group was administerd the extract of Pinus densiflora for 14 days and control group was administerd equal dose of oral. And then we measured the amount of serum triglyceride, Total cholesterol, LDL-cholesterol, HDL-cholesterol, Free Fatty Acid, phospholipid, Insuline, Laptin, Body weight, epididymis fat weight & rate, epididymis fat cell, Cardiac Risk Factor(CRF. The results were as followers : 1. Sample Group showed decreasing effects on Total cholesterol, Trigylceride, LDL-cholesterol, and Phospholipid levels in serum and CRF significantly. 2. Sample Group showed increasing effects on HDL-cholesterol level in serum significantly. 3. Sample Group showed decreasing effects on Insuline in serum significantly. 4. Sample Group showed increasing effects on Laptin in serum significantly. 5. Sample Group showed decreasing effects on Body weight, epididymis fat weight & rate, epididymis fat cell significantly. According to the above results, Pinus densiflora showed significant decreasing effects on hyperlipidemia and lipid in rats, it is considered that it is appropriate to apply for hyperlipidemia, obesity.

  17. Clinal differentiation and putative hybridization in a contact zone of Pinus ponderosa and P. arizonica (Pinaceae).

    Science.gov (United States)

    Epperson, B K; Telewski, F W; Plovanich-Jones, A E; Grimes, J E

    2001-06-01

    The widely distributed Pinus subsection Ponderosae is a species complex that has a transition zone among taxa in the southwestern United States. In southern Arizona and New Mexico at least two recognized taxa, Pinus ponderosa var. scopulorum and Pinus arizonica or P. ponderosa var. arizonica, are known to coexist in close proximity. In this study, we report the existence of populations where the taxa are sympatric. One of the key characteristics distinguishing taxa is the number of needles per fascicle; P. ponderosa typically has three, P. arizonica has five. We examined the spatial distribution of needle-number types in a belt transect that covers a transition zone from nearly pure three-needle types at the top of Mount Lemmon to five-needle types downslope, in the Santa Catalina Mountains, Arizona. The spatial distribution is inconsistent with there being both free interbreeding among types and selective neutrality of types. Trees with intermediate types, having combinations of three, four, and five needles and/or mean numbers of needles between 3.0 and 5.0, are spatially concentrated in the middle of the transition zone. The spatial distribution supports the occurrence of hybridization and introgression, and this is consistent with reported crossabilities of the types. The results suggest that selection is acting, either on needle number per se or on other traits of the ecotype with which it may be in linkage disequilibrium, to maintain the observed steep clinal differentiation.

  18. Spatial pattern of allozyme variation in a contact zone of Pinus Ponderosa and P. arizonica (Pinaceae).

    Science.gov (United States)

    Epperson, Bryan K; Chung, Myong Gi; Telewski, Frank W

    2003-01-01

    The spatial distribution of genotypes for nine polymorphic allozyme loci was examined in a contact zone between Pinus ponderosa var. scopulorum and another tree regarded as either a separate species, Pinus arizonica, or variety, Pinus ponderosa var. arizonica, in southern Arizona. Previous work had identified a steep elevational cline for a key taxonomic trait, number of leaf-needles per fascicle, on the south slope of Mt. Lemmon. The present results indicate that the taxa are not fully interbreeding in this contact zone, because allozyme genotypes are considerably more spatially structured than expected for the dispersal characteristics of pines. The amount of spatial differentiation is also much less than that observed for needle number. It appears that this is due to the lack of differentiation for allozyme gene frequencies for the two types of trees, which is further evidenced by analysis of samples from two other populations away from the contact zone. It is likely that if the two taxa were isolated in the past, it was not for long enough nor complete enough to allow mutation-drift to create substantial differentiation between them. Another possible explanation is that introgression after recontact is so advanced that any differences have been erased throughout the Santa Catalina mountain range.

  19. ECONOMIC ANALYSIS OF THE Pinus elliottii YIELD IN THE SOUTHEASTERN MOUNTAIN RANGE, RIO GRANDE DO SUL

    Directory of Open Access Journals (Sweden)

    Eduardo Pagel Floriano

    2010-03-01

    Full Text Available This study was conducted for Pinus elliottii Engelm. at Serra do Sudeste, Rio Grande do Sul, with the following objectives: to check production costs and possible revenues; to perform the economic and financial analysis of Pinus elliottii's wood production under regional conditions; and to determine the rotation of maximum Net Present Value (NPV. The costs for planting the stands were estimated at $2,292.09/ha and the annual maintenance ranged from R$ 134.84 to R$ 363.98 per hectare. Departing from the Site Index 28, with NPV of R$ 1,147.17/ha, Pinus' wood production becomes interesting. At the IS 26, with 6.86% Internal Rate of Return (IRR, it would be possible to pay the 6.75% Propflora's interest. Analysis of different rotations for IS 28 showed maximum VPL with rotation of 26 years, thinning in cycle of 4 years, starting up the cuts to 10 years. The economic analysis was performed with considerable caution, using productivity moderated levels, costs within patterns that can be considered between middle and high and moderated prices for today's wood market. Even with the restrictions imposed on the analysis, results are promising; especially in view of the regional market prices' increase tendency and the probability of wood veneer industries comes to absorb the larger log's size production in a closed future.

  20. Strenght of the glue line of edge glued panels of Pinus taeda made with different adhesives

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    Merielen de Carvalho Lopes

    2013-12-01

    Full Text Available The study compares the strength of the glue line of edge glued panels of Pinus taeda made with different industrial adhesives. Three types of adhesives (poly(vinyl acetate (PVAc, emulsion polymer isocyanate (EPI and polyurethane (PU glued in two side gluing orientation (radial and tangential and analyzed after the conditioning tests (dry and wet were analyzed. The 360 specimens were prepared for determining the shear strength of edge glued panels of Pinus taeda, and these were tested in a universal testing machine called EMIC. The shear strength of the glue line at dry condition was lower in the tangential side for the three types of adhesives analyzed. The PU adhesive showed the highest values of shear strength in the applied conditions, differing from PVAc and EPI adhesive when tested on dry condition and did not differ from the others adhesives when tested on wet condition. The highest percentage of wood failure was observed after the shear strength test at dry condition to EPI adhesive glued in the radial side. And in the wet condition, the EPI adhesive and PU adhesive glued in tangential side showed higher mean values of wood failure. Thus, it was possible to conclude that the strength of the glue line of Pinus taeda wood was influenced by the type of adhesive and side gluing orientation, and showed differently behavior according to the condition of use (wet or dry.

  1. De Novo Sequencing and Assembly Analysis of Transcriptome in Pinus bungeana Zucc. ex Endl.

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    Qifei Cai

    2018-03-01

    Full Text Available To enrich the molecular data of Pinus bungeana Zucc. ex Endl. and study the regulating factors of different morphology controled by apical dominance. In this study, de novo assembly of transcriptome annotation was performed for two varieties of Pinus bungeana Zucc. ex Endl. that are obviously different in morphology. More than 147 million reads were produced, which were assembled into 88,092 unigenes. Based on a similarity search, 11,692 unigenes showed significant similarity to proteins from Picea sitchensis (Bong. Carr. From this collection of unigenes, a large number of molecular markers were identified, including 2829 simple sequence repeats (SSRs. A total of 158 unigenes expressed differently between two varieties, including 98 up-regulated and 60 down-regulated unigenes. Furthermore, among the differently expressed genes (DEGs, five genes which may impact the plant morphology were further validated by reverse transcription quantitative polymerase chain reaction (RT-qPCR. The five genes related to cytokinin oxidase/dehydrogenase (CKX, two-component response regulator ARR-A family (ARR-A, plant hormone signal transduction (AHP, and MADS-box transcription factors have a close relationship with apical dominance. This new dataset will be a useful resource for future genetic and genomic studies in Pinus bungeana Zucc. ex Endl.

  2. SHEAR STRENGTH IN THE GLUE LINE OF Eucalyptus sp. AND Pinus sp.WOOD

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    Juliana Jerásio Bianche

    Full Text Available ABSTRACT To evaluate the adhesive efficiency on the union of glued joints in a particular temperature and humidity conditions for a specified time the adhesive must be submitted to specific load tests, such as shear in the glue line. The objective of this study was to evaluate the shear strength in the glue line of Eucalyptus sp and Pinus sp.woods. Five adhesives (castor oil, sodium silicate, modified silicate, , PVA and resorcinol-formaldehyde, three weights (150 g/m2, 200 g/m2, and 250 g/m2 and two species (Eucalyptus sp. and Pinus sp. of wood were used. Twelve specimens were obtained from each repetition per treatment, corresponding to 108 specimens that were conditioned at a temperature of 23 ± 1°C and relative humidity of 50 ± 2%. The interaction between the weight and type of adhesive was significant for the shear strength in the glue line of eucalyptus wood. However, no interaction between the weight and the adhesive was found for pinus, only the isolated from the adhesive effect. Chemical bonds originated in the polymerization of resorcinol-formaldehyde adhesives and castor bi-component conferred upon these adhesives the greatest resistance in the glue line. Castor and resorcinol-formaldehyde adhesives showed the highest shear strength values in the line of glue and wood failure. Castor adhesive presented satisfactory performance for bonding of eucalyptus and pine woods.

  3. Distribution of chemical compartments of soil organic matter and c stocks of a cambisol from south Brazil as affected by Pinus afforestation

    Directory of Open Access Journals (Sweden)

    Henrique Cesar Almeida

    2012-01-01

    Full Text Available Distribution and stocks of soil organic matter (SOM compartments after Pinus monoculture introduction in a native pasture area of a Cambisol, Santa Catarina, Brazil, were investigated. Pinus introduction increased soil acidity, content of exchangeable Al+3 and diminished soil nutrients. Nevertheless, soil C stock increased in all humic fractions of the 0-5 cm layer after Pinus afforestation. In the subsurface, the vegetation change only promoted SOM redistribution from the NaOH-extractable humic substances to a less hydrophobic humin fraction. Under Pinus, soil organo-mineral interactions were relevant up to a 15 cm depth, while in pasture environment, this mechanism occurred mainly in the surface layer.

  4. Desarrollo de herramienta de gestión de proyectos RUP usando metodologías Scrum + XP : gestión del proyecto y requisitos

    OpenAIRE

    Molina Romero, Javier; Quishpi Betún, Luis

    2016-01-01

    Una de las principales causas por las que los proyectos de desarrollo software fracasan es la ausencia de metodología en su desarrollo. La motivación que hay detrás de este proyecto es el desarrollo de una aplicación web usando la metodología Rational Unified Process (RUP) y aprender, en una situación real, cómo desarrollar utilizando las metodologías Scrum + XP. El principal objetivo de este proyecto es el seguimiento y documentación de una aplicación web para gestionar proyectos mediant...

  5. Efeito de instruções usando DVD-ROM no desempenho técnico de massoterapia miofascial e sua retenção

    OpenAIRE

    Luiz Francisco Cachoni

    2014-01-01

    Contextualização: Existem poucas investigações sobre recursos didáticos modernos para o ensino de habilidades técnicas de massoterapia. Objetivo: Avaliar o efeito de instruções usando filme no formato DVD associado ao ensino tradicional (aula expositiva com demonstração do instrutor em um voluntário), no aprendizado e na retenção de massagem miofascial (MM). Método: Estudo retrospectivo e duplo cego. Uma fisioterapeuta experiente que atuou como professora (cega ao objetivo do estudo) e 60 fis...

  6. Producción de carbones ultralimpios usando flotación burbujeante y lixiviación con ácidos

    OpenAIRE

    Barraza, Juan; Mejía, Isabel

    2009-01-01

    Los carbones ultralimpios representan una importante materia prima para la elaboración de productos de alto valor agregado tales como fibra de carbono, electrodos, espumas de carbón, entre otros. En este trabajo, carbones ultralimpios con concentraciones menores a 0,50% de ceniza (p/p, base seca, bs) se obtuvieron usando flotación burbujeante en columna y lixiviación con ácidos fluorhídrico (HF) y nítrico (HNO3). Los contenidos de ceniza se redujeron desde 19,60 % en los carbones alimentados ...

  7. MODELACIÓN NUMÉRICA DE PROCESOS DE ESTERILIZACIÓN TÉRMICA DE ALIMENTOS USANDO VOLUMENES DE CONTROL: APROXIMACIÓN CILÍNDRICA

    OpenAIRE

    HÉCTOR J. CIRO-VELÁSQUEZ; CARLOS GONZÁLEZ; EDUARD GARCÍA

    2009-01-01

    Un proceso de esterilización térmica de alimentos fue modelado usando la técnica de diferenciación finita aplicada a volúmenes de control. El modelo de simulación fue desarrollado en coordenadas cilíndricas con un ejemplo de aplicación tomando como referencia un producto cárnico (carne de res). Los resultados de la simulación mostraron que el algoritmo desarrollado es independiente de la red nodal seleccionada, permitiendo cuantificar las curvas de penetración de calor en el punto crítico del...

  8. Valoración de la formación recibida usando un perfil de referencia basado en competencias profesionales

    Directory of Open Access Journals (Sweden)

    Mónica Maldonado Rojas

    Full Text Available Introducción: La formación profesional está hoy en día promoviendo la implementación de opciones educativas basadas en modelos por competencias. El presente trabajo tuvo como propósito establecer un perfil de referencia basado en competencias profesionales para la carrera de Tecnología Médica de la Universidad de Talca y usando éstas como indicadores, conocer la auto percepción que tienen los egresados respecto del grado en que fueron adquiridas durante su formación. Material y métodos: Se realizó una investigación de tipo exploratoria, mediante una encuesta autoaplicada que contenía un conjunto de indicadores que correspondían a competencias establecidas previamente en un perfil de referencia, cuya estructura considera diferentes áreas de desempeño. El perfil fue validado por juicio de expertos, determinando el grado de congruencia a través del coeficiente de Serafine. La construcción de la información se realizó a partir de las repuestas, las que fueron codificadas y representadas en porcentajes y posteriormente analizadas bajo categorías interpretativas. Resultados: La evaluación que se hace considera el porcentaje de egresados que valoró el logro de las competencias por área de desempeño en los niveles más altos. Los resultados obtenidos fueron: Para el área asistencial el 72,9 %, para el área investigación un 59 %, para el área de docencia un 44%, para el área administrativa un 27,1% y para el área personal-social un 91,3 %. Conclusiones: Siguiendo el criterio de categorías interpretativas, el área personal social indica una gran fortaleza de la formación, el área asistencial y el área investigación se encuentran en un nivel satisfactorio y las áreas docencia y administración en niveles medianamente satisfactorio, siendo esta última la gran falencia de la formación. El currículo vigente es adecuado para asegurar el logro de gran número de competencias, pero se debe revisar con el objetivo de

  9. Mejoramiento de imágenes usando funciones de base radial Images improvement using radial basis functions

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    Jaime Alberto Echeverri Arias

    2009-07-01

    Full Text Available La eliminación del ruido impulsivo es un problema clásico del procesado no lineal para el mejoramiento de imágenes y las funciones de base radial de soporte global son útiles para enfrentarlo. Este trabajo presenta una técnica de interpolación que disminuye eficientemente el ruido impulsivo en imágenes, mediante el uso de interpolante obtenido por funciones de base radial en el marco de la investigación enfocada en el desarrollo de un Sistema de recuperación de imágenes de recursos acuáticos amazónicos. Esta técnica primero etiqueta los píxeles de la imagen que son ruidosos y, mediante la interpolación, genera un valor de reconstrucción de dicho píxel usando sus vecinos. Los resultados obtenidos son comparables y muchas veces mejores que otras técnicas ya publicadas y reconocidas. Según el análisis de resultados, se puede aplicar a imágenes con altas tasas de ruido, manteniendo un bajo error de reconstrucción de los píxeles "ruidosos", así como la calidad visual.Global support radial base functions are effective in eliminating impulsive noise in non-linear processing. This paper introduces an interpolation technique which efficiently reduces image impulsive noise by means of an interpolant obtained through radial base functions. These functions have been used in a research project designed to develop a system for the recovery of images of Amazonian aquatic resources. This technique starts with the tagging by interpolation of noisy image pixels. Thus, a value of reconstruction for the noisy pixels is generated using neighboring pixels. The results obtained with this technique have proved comparable and often better than those obtained with previously known techniques. According to results analysis, this technique can be successfully applied on images with high noise levels. The results are low error in noisy pixel reconstruction and better visual quality.

  10. Intra- and interpopulation relationships and taxonomic status of Pinus nigra Arnold in Croatia according to morphology and anatomy of needles

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    Zlatko Liber

    2014-01-01

    Full Text Available Intra- and interpopulational relationships and taxonomic status of six natural black pine populations (Pinus nigra Arnold from Croatia were analyzed. Special attention in this research was paid to the establishment of taxonomic status and distribution of stenoendemic Dalmatian black pine (Pinus nigra Arnold subsp. dalmatica (Vis. Franco. Three morphological and twenty-one anatomical traits of needles were used as well as analysis by the methods of multivariate statistics. Most of the researched individuals showed strong population affiliation, whereas a clinal transition of morphological and anatomical traits of needles was determined among the populations. The opinion of the existence of three subspecies of black pine on the territory of Republic of Croatia (Pinus nigra Arnold subsp. nigra, Pinus nigra Arnold subsp. dalmatica (Vis. Franco and Pinus nigra Arnold subsp. illyrica (Vid. Fukarek was confirmed. For each of the three subspecies new ranges were represented. Four Croatian populations were included in the stenoendemic subspecies of the Dalmatian black pine (Brač, Hvar, Grobnik and Pelješac, and a new distribution, broken in two, was suggested. Since the Dalmatian black pine has been reduced to several populations only, and there are some signs of continuous diminishing of its range, this research points out the need of making an action plan to preserve all natural populations of this subspecies.

  11. Transcriptome resources and functional characterization of monoterpene synthases for two host species of the mountain pine beetle, lodgepole pine (Pinus contorta) and jack pine (Pinus banksiana)

    Science.gov (United States)

    2013-01-01

    Background The mountain pine beetle (MPB, Dendroctonus ponderosae) epidemic has affected lodgepole pine (Pinus contorta) across an area of more than 18 million hectares of pine forests in western Canada, and is a threat to the boreal jack pine (Pinus banksiana) forest. Defence of pines against MPB and associated fungal pathogens, as well as other pests, involves oleoresin monoterpenes, which are biosynthesized by families of terpene synthases (TPSs). Volatile monoterpenes also serve as host recognition cues for MPB and as precursors for MPB pheromones. The genes responsible for terpene biosynthesis in jack pine and lodgepole pine were previously unknown. Results We report the generation and quality assessment of assembled transcriptome resources for lodgepole pine and jack pine using Sanger, Roche 454, and Illumina sequencing technologies. Assemblies revealed transcripts for approximately 20,000 - 30,000 genes from each species and assembly analyses led to the identification of candidate full-length prenyl transferase, TPS, and P450 genes of oleoresin biosynthesis. We cloned and functionally characterized, via expression of recombinant proteins in E. coli, nine different jack pine and eight different lodgepole pine mono-TPSs. The newly identified lodgepole pine and jack pine mono-TPSs include (+)-α-pinene synthases, (-)-α-pinene synthases, (-)-β-pinene synthases, (+)-3-carene synthases, and (-)-β-phellandrene synthases from each of the two species. Conclusion In the absence of genome sequences, transcriptome assemblies are important for defence gene discovery in lodgepole pine and jack pine, as demonstrated here for the terpenoid pathway genes. The product profiles of the functionally annotated mono-TPSs described here can account for the major monoterpene metabolites identified in lodgepole pine and jack pine. PMID:23679205

  12. Response of needle dark respiration of Pinus koraiensis and Pinus sylvestriformis to elevated CO2 concentrations for four growing seasons' exposure

    Institute of Scientific and Technical Information of China (English)

    ZHOU YuMei; HAN ShiJie; ZHANG HaiSen; XIN LiHua; ZHENG JunQiang

    2007-01-01

    The long-term effect of elevated CO2 concentrations on needle dark respiration of two coniferous species-Pinus koraiensis and Pinus sylvestriformis on the Changbai Mountain was investigated using open-top chambers. P. Koraiensis and P. Sylvestriformis were exposed to 700,500μmol·mol-1 CO2 and ambient CO2(approx.350 μmol·mol-1)for four growing seasons. Needle dark respiration was measurd during the second, third and fourth growing seasons' exposure to elevated CO2.The results showed that needle dark respiration rate increased for P. Koraiensis and P. Sylvestriformis grown at elevated CO2 concentrations during the second growing season, could be attributed to the change of carbohydrate and/or nitrogen content of needles. Needle dark respiration of P. Koraiensis was stimulated and that of P. Sylvestriformis was inhibited by elevated CO2 concentrations during the third growing season. Different response of the two tree species to elevated CO2 mainly resulted from the difference in the growth rate. Elevated CO2 concentrations inhibited needle dark respiration of both P. Koraiensis and P. Sylvestriformis during the fourth growing season. There was consistent trend between the short-term effect and the long-term effect of elevated CO2 on needle dark respiration in P. Sylvestriformis during the third growing season by changing measurement CO2 concentrations. However, the short-term effect was different from the long-term effect for P. Koraiensis. Response of dark respiration of P. Koraiensis and P. Sylvestriformis to elevated CO2 concentrations was related to the treatment time of CO2 and the stage of growth and development of plant. The change of dark respiration for the two tree species was determined by the direct effect of CO2 and long-term acclimation. The prediction of the long-term response of needle dark respiration to elevated CO2 concentration based on the short-term response is in dispute.

  13. Transcriptome resources and functional characterization of monoterpene synthases for two host species of the mountain pine beetle, lodgepole pine (Pinus contorta) and jack pine (Pinus banksiana).

    Science.gov (United States)

    Hall, Dawn E; Yuen, Macaire M S; Jancsik, Sharon; Quesada, Alfonso Lara; Dullat, Harpreet K; Li, Maria; Henderson, Hannah; Arango-Velez, Adriana; Liao, Nancy Y; Docking, Roderick T; Chan, Simon K; Cooke, Janice Ek; Breuil, Colette; Jones, Steven Jm; Keeling, Christopher I; Bohlmann, Jörg

    2013-05-16

    The mountain pine beetle (MPB, Dendroctonus ponderosae) epidemic has affected lodgepole pine (Pinus contorta) across an area of more than 18 million hectares of pine forests in western Canada, and is a threat to the boreal jack pine (Pinus banksiana) forest. Defence of pines against MPB and associated fungal pathogens, as well as other pests, involves oleoresin monoterpenes, which are biosynthesized by families of terpene synthases (TPSs). Volatile monoterpenes also serve as host recognition cues for MPB and as precursors for MPB pheromones. The genes responsible for terpene biosynthesis in jack pine and lodgepole pine were previously unknown. We report the generation and quality assessment of assembled transcriptome resources for lodgepole pine and jack pine using Sanger, Roche 454, and Illumina sequencing technologies. Assemblies revealed transcripts for approximately 20,000 - 30,000 genes from each species and assembly analyses led to the identification of candidate full-length prenyl transferase, TPS, and P450 genes of oleoresin biosynthesis. We cloned and functionally characterized, via expression of recombinant proteins in E. coli, nine different jack pine and eight different lodgepole pine mono-TPSs. The newly identified lodgepole pine and jack pine mono-TPSs include (+)-α-pinene synthases, (-)-α-pinene synthases, (-)-β-pinene synthases, (+)-3-carene synthases, and (-)-β-phellandrene synthases from each of the two species. In the absence of genome sequences, transcriptome assemblies are important for defence gene discovery in lodgepole pine and jack pine, as demonstrated here for the terpenoid pathway genes. The product profiles of the functionally annotated mono-TPSs described here can account for the major monoterpene metabolites identified in lodgepole pine and jack pine.

  14. Microwave-assisted extraction and ultrasonic extraction to determine polycyclic aromatic hydrocarbons in needles and bark of Pinus pinaster Ait. and Pinus pinea L. by GC-MS.

    Science.gov (United States)

    Ratola, Nuno; Lacorte, Sílvia; Barceló, Damià; Alves, Arminda

    2009-01-15

    Two different extraction strategies (microwave-assisted extraction (MAE) and ultrasonic extraction (USE)) were tested in the extraction of the 16 US Environmental Protection Agency (EPA) polycyclic aromatic hydrocarbons (PAHs) from pine trees. Extraction of needles and bark from two pine species common in the Iberian Peninsula (Pinus pinaster Ait. and Pinus pinea L.) was optimized using two amounts of sample (1g and 5 g) and two PAHs spiking levels (20 ng/g and 100 ng/g). In all cases, the clean-up procedure following extraction consisted in solid-phase extraction (SPE) with alumina cartridges. Quantification was done by gas chromatography (GC) with mass spectrometry (MS), using five deuterated PAH surrogate standards as internal standards. Limits of detection were globally below 0.2 ng/g. The method was robust for the matrices studied regardless of the extraction procedures. Recovery values between 70 and 130% were reached in most cases, except for high molecular weight PAHs (indeno[1,2,3-cd]pyrene, dibenzo[a,h]anthracene and benzo[ghi]perylene). A field study with naturally contaminated samples from eight sites (four in Portugal and four in Catalonia, Spain) showed that needles are more suitable biomonitors for PAHs, yielding concentrations from 2 to 17 times higher than those found in bark. The levels varied according to the sampling site, with the sum of the individual PAH concentrations between 213 and 1773 ng/g (dry weight). Phenanthrene was the most abundant PAH, followed by fluoranthene, naphthalene and pyrene.

  15. Isoenzymatic variability in progeny of Pinus mugo Turra x Pinus sylvestris L. hybrids from Bór na Czerwonem, in experimental culture

    Directory of Open Access Journals (Sweden)

    Maria A. Bobowicz

    2014-01-01

    Full Text Available The article describes the genetic structure of 12-year old seedlings. They were grown in an experimental culture representing the progeny of natural hybrids of Pinus mugo Turra and Pinus sylvestris L. from the peatbog Bór na Czerwonem. The studies were carried out on seedlings, employing electrophoresis of enzymatic proteins from winter buds. Parallel isoenzymatic analyses were also conducted for trees of P. mugo Turra from Tatra Mountains and for P. sylvestris L. trees from Białowieża in order to determine similarities of the hybrids to the 'pure' species, thought to represent parental forms, and for natural hybrids from Bór na Czerwonem. The obtained electrophoretic patterns made the determination of genetic structure of the population possible, namely of the observed heterozygosity, expected heterozygosity, Wright's fixation index and coefficients of similarity according to Nei and Hedrick. Genetic distances between the populations were also estimated. The variability of 11 enzymatic loci demonstrated the highest level of variability in the population of hybrids. Coefficients of genetic differentiation (GST and of gene flow, (Nm suggested a close relationship of the hybrids to P. mugo which may indicate an introgressive type of the hybrid swarm, from which the studied trees originated. The analysis of genetic similarity coefficients, according to Nei and Hedrick, demonstrated also an extensive similarity of the two hybrid groups to P. mugo. However, upon analysis of individual enzymatic loci, some of them highly resembled P. mugo while others were similar to P. sylvestris. The closest genetic similarity was demonstrated for the group of hybrids from the peatbog and the respective of hybrides group from culture. The variable gene expression as well as asymmetric inheritance of isoenzymes provided additional evidence of the introgressive character of the studied trees from the hybrid population.

  16. 13C discriminations of Pinus sylvestris vs. Pinus ponderosa at a dry site in Brandenburg (eastern Germany): 100-year growth comparison.

    Science.gov (United States)

    Wagner, Ralf; Insinna, Patrick A; Götz, Bernhard; Junge, Sebastian; Boettger, Tatjana

    2007-06-01

    The carbon isotope composition (delta(13)C, per thousand) and discrimination (Delta, per thousand) of old grown North American Pinus ponderosa Dougl. Ex P. et C. Laws. and European Pinus sylvestris L. were determined using trees grown under almost identical growing conditions in a mixed stand in Bralitz, Northeast Germany. Single-tree delta(13)C analyses of tree-ring cellulose of both species were carried out at a yearly resolution for the period 1901-2001 and the results compared with growth (basal area increment). Annual mean delta(13)C values for P. ponderosa ranged from-21.6 per thousand to-25.2 per thousand and for P. sylvestris from-21.4 per thousand to-24.4 per thousand. Accordingly, (13)C discrimination (Delta) showed higher values for P. ponderosa throughout the investigation period. Five characteristic periods of Delta were identified for both the tree species, reflecting positive and negative influences of environmental factors. Good growing conditions such as after-thinning events had a positive effect on Delta, reflecting higher values, while poor conditions like aridity and air pollution had a negative influence, reflecting lower values. The dynamics of Delta were likewise reflected in the growth (basal area increment, BAI). Higher (13)C discrimination values of P. ponderosa led to higher BAIs of P. ponderosa in comparison with P. sylvestris. Correlation function analyses confirmed that P. sylvestris was more dependent on precipitation than P. ponderosa, which showed a closer relationship with temperature. The results confirm that under predominantly dry growing conditions, P. ponderosa showed better growth performance than P. sylvestris, indicating better common intrinsic water-use efficiency and, therefore, higher rates of net photosynthesis at a given transpiration. In view of the prospect of climate change, the results are very significant for assessing both trees' physiological properties and, hence, their potential for coping with future growing

  17. Oviposition Behavior and Survival of Tamarixia radiata (Hymenoptera: Eulophidae), an Ectoparasitoid of the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Liviidae), on Hosts Exposed to an Entomopathogenic Fungus, Isaria fumosorosea (Hypocreales: Cordycipitaceae), Under Laboratory Conditions.

    Science.gov (United States)

    Chow, Andrew; Dunlap, Christopher A; Jackson, Mark A; Flores, Daniel; Patt, Joseph M; Sétamou, Mamoudou

    2016-10-01

    Antagonistic interactions between the nymphal parasitoid, Tamarixia radiata Waterston (Hymenoptera: Eulophidae), and the ARSEF 3581 strain of the entomopathogenic fungus, Isaria fumosorosea Wize (Hypocreales: Cordycipitaceae), could disrupt biological control of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). Three interactions were evaluated under laboratory conditions at 25 °C: 1) parasitoid survival if parasitized hosts were exposed to ARSEF 3581 blastospores before or after host mummification; 2) parasitoid survival if mummies containing larva or pupa were exposed to ARSEF 3581 hyphae; 3) parasitoid oviposition on infected hosts with visible or without visible hyphae. Topical application of blastospore formulation onto the dorsal surfaces of live nymphs parasitized with second-instar wasp larva (3 d after parasitism) reduced host mummification by 50% and parasitoid emergence by 85%. However, parasitoid emergence was not affected by topical application of blastospore formulation onto mummies that contained fourth-instar wasp larva (6 d after parasitism). Parasitoid emergence was reduced by 80% if mummies containing fourth-instar wasp larva were covered with blastospore formulation colonized by fungal hyphae. In comparison, parasitoid emergence was not affected if mummies containing wasp pupa (9 d after parasitism) were covered with formulation colonized by fungal hyphae. Female parasitoids oviposited on infected hosts without visible hyphae but not on infected hosts with visible hyphae. Our findings suggest that I. fumosorosea could detrimentally affect T. radiata, if both natural enemies are simultaneously deployed for biological control of D. citri However, temporal separation of the fungus and parasitoid could reduce antagonism and enhance control of D. citri. © The Authors 2016. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  18. Effect of tillage and crop residues management on mungbean (vigna radiata (L.) wilczek) crop yield, nitrogen fixation and water use efficiency in rainfed areas

    International Nuclear Information System (INIS)

    Mohammad, W.; Shehzadi, S.; Shah, S.M.; Shah, Z.

    2010-01-01

    A field experiment was conducted to study the effect of crop residues and tillage practices on BNF, WUE and yield of mungbean (Vigna radiata (L.) Wilczek) under semi arid rainfed conditions at the Livestock Research Station, Surezai, Peshawar in North West Frontier Province (NWFP) of Pakistan. The experiment comprised of two tillage i) conventional tillage (T1) and ii) no-tillage (T0) and two residues i) wheat crop residues retained (+) and ii) wheat crop residues removed (-) treatments. Basal doses of N at the rate of 20: P at the rate of 60 kg ha-1 were applied to mungbean at sowing time in the form of urea and single super phosphate respectively. Labelled urea having 5% 15N atom excess was applied at the rate of 20 kg N ha-1 as aqueous solution in micro plots (1m2) in each treatment plot to assess BNF by mungbean. Similarly, maize and sorghum were grown as reference crops and were fertilized with 15N labelled urea as aqueous solution having 1% 15N atom excess at the rate of 90 kg N ha/sup -1/. The results obtained showed that mungbean yield (grain/straw) and WUE were improved in notillage treatment as compared to tillage treatment. Maximum mungbean grain yield (1224 kg ha/sup -1/) and WUE (6.61kg ha/sup -1 mm/sup -1/) were obtained in no-tillage (+ residues) treatment. The N concentration in mungbean straw and grain was not significantly influenced by tillage or crop residue treatments. The amount of fertilizer-N taken up by straw and grain of mungbean was higher under no-tillage with residues-retained treatment but the differences were not significant. The major proportion of N (60.03 to 76.51%) was derived by mungbean crop from atmospheric N2 fixation, the remaining (19.6 to 35.91%) was taken up from the soil and a small proportion (3.89 to 5.89%) was derived from the applied fertilizer in different treatments. The maximum amount of N fixed by mungbean (82.59 kg ha/sup -1/) was derived in no-tillage with wheat residue-retained treatment. By using sorghum as

  19. Clasificación digital de masas nubosas a partir de imágenes meteorológicas usando algoritmos de aprendizaje de máquina

    Directory of Open Access Journals (Sweden)

    Salomón Einstein Ramírez-Fernández

    2014-01-01

    Full Text Available La identificación exacta de nubes precipitantes es una tarea difícil. En el presente trabajo se aplicaron los algoritmos Máquinas de Soporte Vectorial, Árboles de Decisión y Bosques Aleatorios para discriminar entre nubes precipitantes y nubes no precipitantes, a partir de una imagen meteorológica del satélite GOES-13 que cubre el territorio colombiano. El objetivo del trabajo fue evaluar el desempeño de los algoritmos de aprendizaje de máquina (ML, para la clasificación digital de masas nubosas, en términos de la exactitud temática de la clasificación usando como referencia el algoritmo convencional distancia de Mahalanobis. Los resultados muestran que los algoritmos ML proporcionan una clasificación de masas de nubes más exacta que la obtenida por algoritmos convencionales. La mejor exactitud fue obtenida usando Bosques Aleatorios (RF, con una exactitud temática global de 97%. Adicionalmente, la clasificación obtenida con RF fue comparada pixel a pixel con estimaciones de precipitación de la NASA Tropical Rainfall Measurement Mission (TRMM obteniendo una exactitud global del 94%. De acuerdo con este estudio, los algoritmos ML pueden ser usados para mejorar los actuales métodos de identificación de nubes precipitantes.

  20. Lei da gravitação universal e os satélites: uma abordagem histórico-temática usando multimídia

    Directory of Open Access Journals (Sweden)

    Elvis Vilela Rodrigues

    2012-01-01

    Full Text Available Examina-se, neste artigo, o desenvolvimento de aulas de Física em que, usando-se uma multimídia, a Lei da Gravitação Universal é abordada de forma contextualizada a partir da História da Ciência e de um tema atual (os satélites. O objetivo foi examinar como estudantes de Ensino Médio aceitam e se envolvem nesse estudo. Usando uma abordagem metodológica qualitativa, a multimídia apresenta o que são e como funcionam os satélites artificiais, trazendo uma narrativa histórica desde as ideias de movimento do sistema planetário de Ptolomeu até as de Isaac Newton sobre o movimento dos corpos, culminando na Lei da Gravitação Universal. Entrevistas, observações de sala de aula e documentos produzidos pelos estudantes mostram que as imagens, os filmes e os textos contidos na multimídia enriquecem, de modo significativo, o conteúdo, facilitando o entendimento de conceitos da Física. A contextualização histórico-temática, por sua vez, produz maior envolvimento dos alunos no estudo da Física.