WorldWideScience

Sample records for pineapples

  1. US SEEDLESS PINEAPPLE ORANGE

    Science.gov (United States)

    The Agricultural Research Service, U.S. Department of Agriculture, hereby releases for propagation the US SEEDLESS PINEAPPLE citrus scion selection, formerly tested as USDA 1-10-60. US SEEDLESS PINEAPPLE resulted from irradiation of Ridge Pineapple seeds by C.J. Hearn in 1970 at the U.S. Horticultu...

  2. Pineapple [Ananas comosus (L.) Merr].

    Science.gov (United States)

    Gangopadhyay, Gaurab; Mukherjee, Kalyan K

    2015-01-01

    The efficacy of Agrobacterium-mediated pineapple transformation technique has been improved (mean percentage of transgenic micro-shoots regenerated from initial callus explants up to 20.6%) using a novel encapsulation-based, antibiotic selection procedure. The detailed protocol using a standard plant transformation vector (pCAMBIA1304) as reported in an 'elite' Indian variety (Queen) of pineapple [Ananas comosus (L.) Merr] can be applied to other varieties of pineapple for introgression of target genes.

  3. Alcohol production from pineapple waste

    Energy Technology Data Exchange (ETDEWEB)

    Ban-Koffi, L. (Ministry of Scientific Research, Abidjan (CI). Ivorian Center of Technological Research); Han, Y.W. (USDA, Southern Regional Research Center, New Orleans, LA (US))

    1990-09-01

    Saccharomyces cerevisiae and Zymomonas mobilis were grown on pineapple waste and their alcohol production characteristics compared. The pineapple waste consisted of 19% cellulose, 22% hemi-cellulose, 5% lignin and 53% cell soluble matters but concentration of soluble sugars, which included 5.2% sucrose, 3.1% glucose and 3.4% fructose, was relatively low and pretreatment of the substrate was needed. Pretreatment of pineapple waste with cellulase and hemi-cellulase and then fermentation with S. cerevisiae or Z. mobilis produced about 8% ethanol from pineapple waste in 48 h. (author).

  4. [New cerebroside from leaves of pineapple].

    Science.gov (United States)

    Wang, Jin-Ping; Wang, Hong-Ying; Du, Li-Jun; Ding, Yi; Xing, Dong-Ming; Wang, Wei

    2007-03-01

    To study the chemical constituents of the leaves of pineapple. Chromatographic methods were used to isolate compounds from the leaves of pineapple and spectral methods were used to identify the structures of the isolated compounds. Compound 1 was isolated from the leaves of pineapple. It was identified as 1-O-beta-D-glucopyranosyl-(2S, 3R, 4E, 11E)-2-[(2(R)-hydroxydocosanoyl) amido]-4, 11-hexadecanediene-1, 3-diol. Compound 1 was a new compound.

  5. 21 CFR 145.181 - Artificially sweetened canned pineapple.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Artificially sweetened canned pineapple. 145.181... § 145.181 Artificially sweetened canned pineapple. (a) Artificially sweetened canned pineapple is the food that conforms to the definition and standard of identity prescribed for canned pineapple by § 145...

  6. Towards Sustainable Use of Potassium in Pineapple Waste

    OpenAIRE

    Ahmed, Osumanu H.; Husni, M.H.A.; Anuar, A.R.; Hanafi, M.M.

    2004-01-01

    Due to the 1997/98 haze problem in South-East Asia and the increasing need for sustainable food production and development, the usual management of crop residues (including pineapple wastes) through burning is prohibited. As a result, the need for alternative uses of pineapple wastes in pineapple production has been emphasized. This study investigated an environmentally friendly means of recycling pineapple leaves for agricultural use. Pineapple leaves were shredded and composted in a compost...

  7. Exploring Oven-drying Technique in Producing Pineapple Powder

    OpenAIRE

    Cyril John A. Domingo; Wilma M. De Vera; Raquel C. Pambid

    2017-01-01

    Pineapple puree and juice of 11 to 12 °Brix were used to obtain pineapple powder using oven-drying technique. Addition of maltodextrin in treatments 2 and 4 yielded good quality powder, however addition of sugar and maltodextrin in treatments 1 and 3 resulted to sticky product which was processed to pineapple leather. Treatment 2 composed of pineapple puree and maltodextrin resulted to significantly higher powder recovery compared with treatment 4 which composed of pineapple juice...

  8. Pineapple Fruit Collapse: Newly Emerging Disease of Pineapple Fruit in Lampung, Indonesia

    OpenAIRE

    Joko Prasetyo; Titik Nur Aeny

    2014-01-01

    ABSTRACT Pineapple fruit collapse: newly emerging disease of pineapple fruit in Lampung, Indonesia Recently, a new disease on pineapple fruit has occurred in Lampung. Symptoms of the disease are complex. Fruits rotted and exuded copious liquid from the inter- fruitlet tissues accompanied by gas bubbles. Open spaces were formed inside the rotten fruit. Dissection of diseased fruit showed many cavities within its sceletal fibres and bad odour was exerted from the rotten tissues. A bacterial...

  9. Genomic analyses of the CAM plant pineapple.

    Science.gov (United States)

    Zhang, Jisen; Liu, Juan; Ming, Ray

    2014-07-01

    The innovation of crassulacean acid metabolism (CAM) photosynthesis in arid and/or low CO2 conditions is a remarkable case of adaptation in flowering plants. As the most important crop that utilizes CAM photosynthesis, the genetic and genomic resources of pineapple have been developed over many years. Genetic diversity studies using various types of DNA markers led to the reclassification of the two genera Ananas and Pseudananas and nine species into one genus Ananas and two species, A. comosus and A. macrodontes with five botanical varieties in A. comosus. Five genetic maps have been constructed using F1 or F2 populations, and high-density genetic maps generated by genotype sequencing are essential resources for sequencing and assembling the pineapple genome and for marker-assisted selection. There are abundant expression sequence tag resources but limited genomic sequences in pineapple. Genes involved in the CAM pathway has been analysed in several CAM plants but only a few of them are from pineapple. A reference genome of pineapple is being generated and will accelerate genetic and genomic research in this major CAM crop. This reference genome of pineapple provides the foundation for studying the origin and regulatory mechanism of CAM photosynthesis, and the opportunity to evaluate the classification of Ananas species and botanical cultivars. © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  10. Determinants of Revenue Derived from Pineapple Marketing in Edo ...

    African Journals Online (AJOL)

    Determinants of Revenue Derived from Pineapple Marketing in Edo State, Nigeria. ... The results showed that an average pineapple marketer has a mean volume of 98 dozens and incurred a variable ... EMAIL FULL TEXT EMAIL FULL TEXT

  11. Biomethanation of banana peel and pineapple waste

    Energy Technology Data Exchange (ETDEWEB)

    Bardiya, N.; Somayaji, D.; Khanna, S. [Tata Energy Research Inst., New Delhi (India)

    1997-10-01

    Biomethanation of banana peel and pineapple wastes studied at various HRTs showed a higher rate of gas production at lower retention time. The lowest possible HRT for banana peel was 25 days, resulting in a maximum rate of gas production of 0.76 vol/vol/day with 36% substrate utilization, while pineapple-processing waste digesters could be operated at 10 days HRT, with a maximum rate of gas production of 0.93 vol/vol/day and 58% substrate utilization. For pineapple-processing waste lowering of retention time did not affect the methane content significantly; however, with banana peel an HRT below 25 days showed a drastic reduction in methane content. (author)

  12. Pineapple Fruit Collapse: Newly Emerging Disease of Pineapple Fruit in Lampung, Indonesia

    Directory of Open Access Journals (Sweden)

    Joko Prasetyo

    2014-03-01

    Full Text Available ABSTRACT Pineapple fruit collapse: newly emerging disease of pineapple fruit in Lampung, Indonesia Recently, a new disease on pineapple fruit has occurred in Lampung. Symptoms of the disease are complex. Fruits rotted and exuded copious liquid from the inter- fruitlet tissues accompanied by gas bubbles. Open spaces were formed inside the rotten fruit. Dissection of diseased fruit showed many cavities within its sceletal fibres and bad odour was exerted from the rotten tissues. A bacterial entity was isolated  from the diseased materials. In a pathogenicity test, the isolated bacteria caused the same symptom as mentioned. In the growing-on test the crown of the heavily infected fruit  showed  heart rot symptom.  Those  indicated that the disease was pineapple fruit collapse. Both symptoms were known related to the same causal agent, Erwinia chrysanthemi (pineapple strain Dickeya sp.. In our opinion, this is the first report of pineapple fruit collapse in Indonesia.

  13. Exploring Oven-drying Technique in Producing Pineapple Powder

    Directory of Open Access Journals (Sweden)

    Cyril John A. Domingo

    2017-11-01

    Full Text Available Pineapple puree and juice of 11 to 12 °Brix were used to obtain pineapple powder using oven-drying technique. Addition of maltodextrin in treatments 2 and 4 yielded good quality powder, however addition of sugar and maltodextrin in treatments 1 and 3 resulted to sticky product which was processed to pineapple leather. Treatment 2 composed of pineapple puree and maltodextrin resulted to significantly higher powder recovery compared with treatment 4 which composed of pineapple juice and maltodextrin. The solubility of pineapple powder improved as maltodextrin concentration is increased from 40.00 % to 60.00 %.Addition of maltodextrin also reduced stickiness of the final product. An instant pineapple powder of 5.47 and 5.33 % moisture content could be produced by oven-drying.This level of moisture content will prohibit bacterial growth in the pineapple powder but may have mold or yeast growth with increase storage period at environments with high humidity. Molds were observed on the 17th day at 89.00 % relative humidity as exhibited by the moisture sorption isotherm data. This suggests that appropriate packaging with moisture barrier is recommended for pineapple powder. This study showedthat by using appropriate ratio of juice, puree, and maltodextrin and appropriate oven drying conditions, a good oven-dried pineapple powder could be obtained.

  14. Isozyme variation in wild and cultivated pineapple

    Science.gov (United States)

    Isozyme variation was studied in 161 accessions of pineapple including four species of Ananas and one of Pseudananas. Six enzyme systems (ADH, GPI, PGM, SKDH, TPI, UGPP) involving seven putative loci revealed 35 electromorphs . Considerable variation exists within and between species of Ananas. Sixt...

  15. Cloning and expression of pineapple sucrosephosphate synthase ...

    African Journals Online (AJOL)

    A 1132-base pairs (bp) polymerase-chain-reaction product of sucrose-phosphate synthase (SPS) (EC 2.3.1.14) from pineapple (Ananas comosus cv. Comte de paris) fruit was cloned and nominated as Ac- SPS1. The sequence encodes a putative 377 amino acids protein containing two serine conserved features that had ...

  16. The Pineapple Value Chain in Ethiopia

    NARCIS (Netherlands)

    S. Drost (Sarah); J.C.A.C. van Wijk (Jeroen)

    2011-01-01

    textabstractThis report investigates the dynamics of a multi-stakeholder platform (named: coordination group, or CG) for stakeholders of the pineapple value chains in Ethiopia. The CG was initiated by the Dutch development organisation SNV in 2005 as part of a broader programme to improve market

  17. Analysis of pineapple production systems in Benin

    NARCIS (Netherlands)

    Fassinou Hotegni, V.N.; Lommen, W.J.M.; Vorst, van der J.G.A.J.; Agbossou, E.K.; Struik, P.C.

    2012-01-01

    In Benin, pineapple is an important fruit crop, mainly grown in the Atlantic department. The overall quality of the two cultivars grown, ‘Sugarloaf’ and ‘Smooth Cayenne’, does not meet the requirements for some outlets and the heterogeneity in fruit quality within and between lots is high. This

  18. Microarray analysis of gene expression profiles in ripening pineapple fruits.

    Science.gov (United States)

    Koia, Jonni H; Moyle, Richard L; Botella, Jose R

    2012-12-18

    Pineapple (Ananas comosus) is a tropical fruit crop of significant commercial importance. Although the physiological changes that occur during pineapple fruit development have been well characterized, little is known about the molecular events that occur during the fruit ripening process. Understanding the molecular basis of pineapple fruit ripening will aid the development of new varieties via molecular breeding or genetic modification. In this study we developed a 9277 element pineapple microarray and used it to profile gene expression changes that occur during pineapple fruit ripening. Microarray analyses identified 271 unique cDNAs differentially expressed at least 1.5-fold between the mature green and mature yellow stages of pineapple fruit ripening. Among these 271 sequences, 184 share significant homology with genes encoding proteins of known function, 53 share homology with genes encoding proteins of unknown function and 34 share no significant homology with any database accession. Of the 237 pineapple sequences with homologs, 160 were up-regulated and 77 were down-regulated during pineapple fruit ripening. DAVID Functional Annotation Cluster (FAC) analysis of all 237 sequences with homologs revealed confident enrichment scores for redox activity, organic acid metabolism, metalloenzyme activity, glycolysis, vitamin C biosynthesis, antioxidant activity and cysteine peptidase activity, indicating the functional significance and importance of these processes and pathways during pineapple fruit development. Quantitative real-time PCR analysis validated the microarray expression results for nine out of ten genes tested. This is the first report of a microarray based gene expression study undertaken in pineapple. Our bioinformatic analyses of the transcript profiles have identified a number of genes, processes and pathways with putative involvement in the pineapple fruit ripening process. This study extends our knowledge of the molecular basis of pineapple fruit

  19. Biogas production from solid pineapple waste

    Energy Technology Data Exchange (ETDEWEB)

    Tanticharoen, M.; Bhumiratana, S.; Tientanacom, S.; Pengsobha, L.

    1984-01-01

    Solid pineapple waste composed of shell and core was used as substrate in anaerobic fermentation producing CH4. The experiments were carried out using four 30-L vessels and no mixing, a 200-L plug-flow reactor, and a 5-cubic m stirred tank. Because of high acidity of the substrate, the loading rate is as low as 2.5 g dry solid added/L-day. The average gas yield is 0.3-0.5 L/g dry substrate. A pretreatment of wet solid with sludge effluent prior loading to the digester resulted in better stability of the biodigester than without pretreatment. These studies showed that loading rate can be much higher than those previously used. The 2-stage process was tested to determine a conversion efficiency of high loading and at much shorter reactor retention times. The results of the entire program indicated that biogas production from cannery pineapple waste is technically feasible.

  20. Metabolite profiling and volatiles of pineapple wine and vinegar obtained from pineapple waste.

    Science.gov (United States)

    Roda, Arianna; Lucini, Luigi; Torchio, Fabrizio; Dordoni, Roberta; De Faveri, Dante Marco; Lambri, Milena

    2017-08-15

    Vinegar is an inexpensive commodity, and economic considerations require that a relatively low-cost raw material be used for its production. An investigation into the use of a new, alternative substrate - pineapple waste - is described. This approach enables the utilization of the pineapple's (Ananas comosus) peels and core, which are usually discarded during the processing or consumption of the fruit. Using physical and enzymatic treatments, the waste was saccharified, and the resulting substrate was fermented with Saccharomyces cerevisiae for 7-10days under aerobic conditions at 25°C. This resulted in an alcohol yield of approximately 7%. The alcoholic medium was then used as a seed broth for acetic fermentation using Acetobacter aceti as the inoculum for approximately 30days at 32°C to obtain 5% acetic acid. Samples were analyzed at the beginning and end of the acetification cycle to assess the volatile and fixed compounds by GC-MS and UHPLC-QTOF-MS. The metabolomic analysis indicated that l-lysine, mellein, and gallic acid were significantly more concentrated in the pineapple vinegar than in the original wine. Higher alcohols, aldehydes, and ketones characterized the aroma of the final pineapple vinegar, whilst off-flavors were significantly reduced relative to the initial wine. This study is the first to highlight the metabolite profile of fruit vinegar with a slight floral aroma profile derived from pineapple waste. The potential to efficiently reduce the post-harvest losses of pineapple fruits by re-using them for products with added food values is also demonstrated. Copyright © 2017 Elsevier Ltd. All rights reserved.

  1. UTILIZATION OF PINEAPPLE WASTE AS CARBON SOURCE

    Directory of Open Access Journals (Sweden)

    Abdullah Moch Busairi

    2012-02-01

    Full Text Available The liquid pineapple waste contains mainly sucrose, glucose, fructose and other nutrients. It therefore can potentially be used as carbon source for organic acid fermentation.  The objective of this work is to evaluate the use of pineapple waste as substrate for lactic acid fermentation under variables of aerobic, anaerobic condition and pH controlling. Initial results showed that the liquid pineapple waste can be used as carbon source for lactic acid fermentation using Lactobacillus delbrueckii. In the anaerobic condition growth of bacteria and lactic acid production better than aerobic condition. In the anaerobic condition and the controlled pH  the production of lactic acid are found to be 54.79 g/l  (78.27% yield at  40oC, pH 6, 50 rpm and 70 g/l sugar concentration.  In contrast, only 13.87g/l lactic acid produced if the fermentation pH was not controlled even though the fermentation parameters were kept at the same conditions

  2. Storage of pineapple fruits under different conditions: implication on ...

    African Journals Online (AJOL)

    An experiment was carried out at Abeokuta to investigate the effects of storage conditions popularly used in Abeokuta metropolis on freshly harvested pineapple fruits. The pineapple fruits were harvested from a farmer's field and stored for 40 days under three conditions: refridgeration (mean temperature 10 0 C), Ambient ...

  3. Problems and Prospects of Pineapple Production in Enugu State ...

    African Journals Online (AJOL)

    The study identified problems and prospects of pineapple production in Enugu State of Nigeria. Purposive sampling technique was used to select eighty (80) pineapple farmers from two agricultural zones. Data were analyzed using percentage and mean score. Results showed that greater proportion of the farmers was ...

  4. The pineapple genome and the evolution of CAM photosynthesis

    Science.gov (United States)

    Pineapple (Ananas comosus (L.) Merr.) is the most economically valuable crop possessing crassulacean acid metabolism (CAM), a photosynthetic carbon assimilation pathway with high water-use efficiency, and the second most important tropical fruit. We sequenced the genomes of pineapple varieties F153 ...

  5. 6823 Volume 12 No. 6 October 2012 PROCESSING PINEAPPLE ...

    African Journals Online (AJOL)

    CRSP

    2012-10-06

    Oct 6, 2012 ... PROCESSING PINEAPPLE PULP INTO DIETARY FIBRE ... investigate the processing of pineapple pulp waste from a processing plant, into a ... classified dietary fibre chemically as cellulose, hemicellulose and lignin constituents .... drying time was shorter compared to the freeze-drying and yielded a ...

  6. CONTINUOUS STALL FEEDING OF PINEAPPLE SILAGE AS THE ...

    African Journals Online (AJOL)

    Dairy animals received silage made from pineapple plants as their only source of roughage for a prolonged period. .... ration. Attempts were made to correct the abnormalities that developed. During the course of the experiment prolonged vitamin A supplementation resulted in no ... deficiency specific to the pineapple plant.

  7. A novel approach for rapid micropropagation of maspine pineapple ...

    African Journals Online (AJOL)

    A novel approach for rapid micropropagation of maspine pineapple ( Ananas comosus L.) shoots using liquid shake culture system. ... Maspine (Ananas comosus L.) is currently the most preferred pineapple variety in Malaysia due to its pleasant aroma and applicability in caning. Large quantities of plant materials are ...

  8. Effect of cellulose-based fibers extracted from pineapple (Ananas ...

    African Journals Online (AJOL)

    New polyurethane foams were fabricated utilizing cellulose-based fibers extracted from pineapple (Ananas comosus) leaf as raw material. The pineapple leaf fibers (PALF) were treated with alkali and subsequently bleached to enhance its fiber-matrix adhesion. Polyurethane composites have been prepared by ...

  9. Effect of Processing on the Quality of Pineapple Juice

    NARCIS (Netherlands)

    Hounhouigan, M.H.; Linnemann, A.R.; Soumanou, M.M.; Boekel, van M.A.J.S.

    2014-01-01

    Pineapple processing plays an important role in juice preservation. Because the quality of the pineapple juice is affected by the processing technology applied, the effects of pasteurization and other preservation methods on the overall juice quality were discussed. During juice processing,

  10. Training Needs of Pineapple Farmers in Enugu State, Nigeria ...

    African Journals Online (AJOL)

    Majority of the respondents indicated that training on pineapple should be organized by researchers (65%) through interpersonal communication (83.8%) using ... informal training especially in the areas of processing, preservation and off-season production of pineapple so that they can face challenges of the enterprise, ...

  11. Effect of detachment time of pineapple ( Ananas comosus L .) crown ...

    African Journals Online (AJOL)

    A limiting factor to large scale production of pineapple is the scarcity of planting materials. The use of pineapple crown as a propagation material is common, but with no regard to length of time after detachment. A study was conducted in the late seasons of 2007 and 2008 at the Teaching and Research Farm Ekiti State ...

  12. Phylogeography of pink pineapple mealybugs, Dysmicoccus brevipes (Cockerell) reveals the history of pineapple introduction and cultivation in China.

    Science.gov (United States)

    He, Y B; Zhan, R L; Sun, G M; Wu, J B; Zhao, Y L

    2015-08-19

    The pink pineapple mealybug (PPM), Dysmicoccus brevipes (Cockerell) (Hemiptera: Pseudococcidae), is a widespread plant-sucking insect of considerable concern because it transmits the pineapple mealybug wilt-associated virus. Its distribution is closely linked with its host, the pineapple [Ananas comosus (L.) Merrill] because of its wingless and parthenogenetic characteristics. To investigate the history of D. brevipes introduction and the cultivation of pineapple in China, samples of D. brevipes were collected from the main pineapple production region in China, and from Thailand, and the mitochondrial cytochrome c oxidase subunit I (COI) gene was analyzed. Homologous sequences of D. brevipes COI from Brazil, Thailand, and Philippines that are deposited in GenBank were compared. Phylogenetic analyses suggest there are close genetic relationships between PPM populations from Hawaii, Brazil, the Philippines, and from Thailand and China, which probably originate from South America. It is suggested that most PPMs in China were introduced from South America by way of Southeast Asia, being accompanied by the pineapple seedling. Conversely, some PPMs represented by Haplotype-WN from Wanning of China, and Lampang of Thailand were found to differ greatly from populations in Hawaii, Brazil, the Philippines, Thailand, and China. It is possible that another route was used for the introduction and distribution of pineapple, or that pineapple might have originated in Southeast Asia.

  13. Bioactive compounds and antioxidant activity analysis of Malaysian pineapple cultivars

    Science.gov (United States)

    Chiet, Chong Hang; Zulkifli, Razauden Mohamed; Hidayat, Topik; Yaakob, Harisun

    2014-03-01

    Pineapple industry is one of the important agricultural sectors in Malaysia with 76 cultivars planted throughout the country. This study aims to generate useful nutritional information as well as evaluating antioxidant properties of different pineapple commercial cultivars in Malaysia. The bioactive compound content and antioxidant capacity of `Josapine', `Morris' and `Sarawak' pineapple (Ananas comosus) were studied. The pineapple varieties were collected at commercial maturity stage (20-40% yellowish of fruit peel) and the edible portion of the fruit was used as sample for evaluation. The bioactive compound of the fruit extracts were evaluated by total phenolic and tannin content assay while the antioxidant capacity was determined by ferric reducing antioxidant power (FRAP). From the results obtained, total phenolic and tannin content was highest for `Josapine' followed by `Morris' and `Sarawak'. With respect to FRAP, `Josapine' showed highest reducing capacity, followed by `Morris' and then `Sarawak' having the least value. The bioactive compounds content are positively correlated with the antioxidant capacities of the pineapple extracts. This result indicates that the total phenolics and tannin content present in the pineapples may contribute to the antioxidant capacity of the pineapples.

  14. [Studies on phenolic constituents from leaves of pineapple (Ananas comosus)].

    Science.gov (United States)

    Wang, Wei; Ding, Yi; Xing, Dong-ming; Wang, Jin-ping; Du, Li-jun

    2006-08-01

    To study the phenolic constituents of the leaves of pineapple. Chromatographic methods were used to isolate compounds from the leaves of pineapple and spectroscopic methods were used to identify the structures of the isolated compounds. 7 compounds, ananasate (1), 1-O-caffeoylglycerol (2), 1-O-p-coumaroylglycerol (3), caffeic acid (4), p-coumaric acid (5), beta-sitosterol (6) and daucosterol (7), were isolated from the leaves of pineapple. 1 was a new compound, and others were obtained from this plant for the first time.

  15. CHARACTERISATION OF SOLID AND LIQUID PINEAPPLE WASTE

    Directory of Open Access Journals (Sweden)

    Abdullah Abdullah

    2011-07-01

    Full Text Available The pineapple waste is contain high concentration of biodegradable organic material and suspended solid. As a result it has a high BOD and extremes of pH conditions. The pineapple wastes juice contains mainly sucrose, glucose, fructose and other nutrients. The characterisation this waste is needed to reduce it by  recycling to get raw material or  for  conversion into useful product of higher value added products such as organic acid, methane , ethanol, SCP and enzyme. Analysis of sugar indicates that liquid waste contains mainly sucrose, glucose and fructose.  The dominant sugar was fructose, glucose and sucrose.  The fructose and glucose levels were similar to each other, with fructose usually slightly higher than glucose. The total sugar and citric acid content were 73.76 and 2.18 g/l. The sugar content in solid waste is glucose and fructose was 8.24 and 12.17 %, no sucrose on this waste

  16. Nutrient enrichment of pineapple waste using Aspergillus niger and ...

    African Journals Online (AJOL)

    Nutrient enrichment of pineapple waste using Aspergillus niger and Trichoderma viride by solid state fermentation. Evans Otieno Omwango, Eliud Nyaga Mwaniki Njagi, George Owino Orinda, Ruth Nduta Wanjau ...

  17. World pineapple production: an overview | M F | African Journal of ...

    African Journals Online (AJOL)

    The economic importance of pineapple is easily justified by its unique ... This perishable fruit is usually stored only for 4-5 days after harvesting in normal conditions. ... Hawaii, India, Bangladesh, Nigeria, Kenya, Democratic Republic of Congo, ...

  18. Growth, flowering and fruiting in vitro pineapple (Ananas comosus L ...

    African Journals Online (AJOL)

    pc star

    2013-04-10

    Apr 10, 2013 ... ... under natural conditions is favoured by shortened day-length and cool night temperatures (Van Overbeek and Cruzado, 1948; ..... pineapple plants after acclimatization b) flower of formation c) growth flower d) formation fruit ...

  19. tracing uganda's global primary organic pineapple value chain

    African Journals Online (AJOL)

    ACSS

    2016-02-22

    Feb 22, 2016 ... methods were used to validate results obtained from the .... TABLE 2. Agronomic information on organic pineapple production in Uganda ..... management, which makes the value chain expensive ..... A handbook for value ...

  20. Processing pineapple pulp into dietary fibre supplement | Ackom ...

    African Journals Online (AJOL)

    Processing pineapple pulp into dietary fibre supplement. ... The pasting characteristics or properties of wheat flour fortified with the product up to 20 ... of some popular foods to help increase the fibre intake and health of the general population.

  1. The Economic Potentials of Pineapple Marketing in Edo State, Nigeria.

    African Journals Online (AJOL)

    The Economic Potentials of Pineapple Marketing in Edo State, Nigeria. ... Agricultural marketing involves numerous lines of activities, which if well developed can sustain livelihood. It is in line with this ... EMAIL FULL TEXT EMAIL FULL TEXT

  2. Assessment of feeding value of vegetable-carried pineapple fruit ...

    African Journals Online (AJOL)

    Dr Asaolu

    2015-08-03

    Aug 3, 2015 ... pineapple fruit wastes to Red Sokoto goats in. Ogbomoso, Oyo State of ..... with vegetable carrier as the main treatment effect and mixing ratios as the block. ...... In: The roles of protozoa and fungi in ruminants' digestion.

  3. Assessment of feeding value of vegetable-carried pineapple fruit ...

    African Journals Online (AJOL)

    This study compared the sun-drying characteristics of five blends each (w/w; 1:1, 1:1.5, 1:2, 1:2.5, 1:3) of wheat offal-carried pineapple waste (WO:PW) and brewers' dried grains-carried pineapple waste (BDG:PW), assessed the blends for their nutrient contents and the feeding value of the optimum blends with Red Sokoto ...

  4. Aroma Volatile Compounds from Two Fresh Pineapple Varieties in China

    Directory of Open Access Journals (Sweden)

    Chang-Bin Wei

    2012-06-01

    Full Text Available Volatile compounds from two pineapples varieties (Tainong No.4 and No.6 were isolated by headspace solid phase microextraction (HS-SPME and identified and quantified by gas chromatography-mass spectrometry (GC/MS. In the Tainong No. 4 and No. 6 pineapples, a total of 11 and 28 volatile compounds were identified according to their retention time on capillary columns and their mass spectra, and quantified with total concentrations of 1080.44 µg·kg−1 and 380.66 µg·kg−1 in the Tainong No.4 and No. 6 pineapples, respectively. The odor active values (OAVs of volatile compounds from pineapples were also calculated. According to the OAVs, four compounds were defined as the characteristic aroma compounds for the Tainong No. 4 pineapple, including furaneol, 3-(methylthiopropanoic acid methyl ester, 3-(methylthiopropanoic acid ethyl ester and δ-octalactone. The OAVs of five compounds including ethyl-2-methylbutyrate, methyl-2-methylbutyrate, 3-(methylthiopropanoic acid ethyl ester, ethyl hexanoate and decanal were considered to be the characteristic aroma compounds for the Tainong No. 6 pineapple.

  5. Aroma volatile compounds from two fresh pineapple varieties in China.

    Science.gov (United States)

    Zheng, Liang-Yong; Sun, Guang-Ming; Liu, Yu-Ge; Lv, Ling-Ling; Yang, Wen-Xiu; Zhao, Wei-Feng; Wei, Chang-Bin

    2012-01-01

    Volatile compounds from two pineapples varieties (Tainong No.4 and No.6) were isolated by headspace solid phase microextraction (HS-SPME) and identified and quantified by gas chromatography-mass spectrometry (GC/MS). In the Tainong No. 4 and No. 6 pineapples, a total of 11 and 28 volatile compounds were identified according to their retention time on capillary columns and their mass spectra, and quantified with total concentrations of 1080.44 μg·kg(-1) and 380.66 μg·kg(-1) in the Tainong No.4 and No. 6 pineapples, respectively. The odor active values (OAVs) of volatile compounds from pineapples were also calculated. According to the OAVs, four compounds were defined as the characteristic aroma compounds for the Tainong No. 4 pineapple, including furaneol, 3-(methylthio)propanoic acid methyl ester, 3-(methylthio)propanoic acid ethyl ester and δ-octalactone. The OAVs of five compounds including ethyl-2-methylbutyrate, methyl-2-methylbutyrate, 3-(methylthio)propanoic acid ethyl ester, ethyl hexanoate and decanal were considered to be the characteristic aroma compounds for the Tainong No. 6 pineapple.

  6. In vitro mutagenesis for the improvement of Josapine pineapple

    International Nuclear Information System (INIS)

    Rusli Ibrahim; Amir Hamzah

    2006-01-01

    Pineapple is the most important fruit in terms of revenue earner in Malaysia. There are about 10,000 ha cultivated with this fruit and half of this is owned by estates and planted for the canning industry. The export of canned pineapple is about 2 million standard cases annually valued at RM 60 million, while the export of fresh pineapple is about 40,000 tonnes worth about RM 10 million. The industry for canning is however, an ailing industry with production on the decline since the 70s. Somaclonal variations and induced mutation using irradiation in breeding are least invasive in changes to genetic make-up of an established variety and will be useful for improving the pineapple varieties. The use of tissue culture to generate somaclones with minute genetic changes that do not damage the overall varietal identity would be the most suitable tool to improve the variety. Protocols for the production of tissue culture plantlets of pineapple using bioreactor technology has been developed and proved to be much more efficient and productive compared to conventional method. In vitro mutagenesis using adventitious buds had produced new plants with smooth leaves, vigorous growth and ornamental-like characters. A total of 30,000 plants derived from tissue culture will be planted and screened in the field for the improvement of Josapine pineapple against bacterial heart rot disease and multiple crown. (Author)

  7. The pineapple genome and the evolution of CAM photosynthesis.

    Science.gov (United States)

    Ming, Ray; VanBuren, Robert; Wai, Ching Man; Tang, Haibao; Schatz, Michael C; Bowers, John E; Lyons, Eric; Wang, Ming-Li; Chen, Jung; Biggers, Eric; Zhang, Jisen; Huang, Lixian; Zhang, Lingmao; Miao, Wenjing; Zhang, Jian; Ye, Zhangyao; Miao, Chenyong; Lin, Zhicong; Wang, Hao; Zhou, Hongye; Yim, Won C; Priest, Henry D; Zheng, Chunfang; Woodhouse, Margaret; Edger, Patrick P; Guyot, Romain; Guo, Hao-Bo; Guo, Hong; Zheng, Guangyong; Singh, Ratnesh; Sharma, Anupma; Min, Xiangjia; Zheng, Yun; Lee, Hayan; Gurtowski, James; Sedlazeck, Fritz J; Harkess, Alex; McKain, Michael R; Liao, Zhenyang; Fang, Jingping; Liu, Juan; Zhang, Xiaodan; Zhang, Qing; Hu, Weichang; Qin, Yuan; Wang, Kai; Chen, Li-Yu; Shirley, Neil; Lin, Yann-Rong; Liu, Li-Yu; Hernandez, Alvaro G; Wright, Chris L; Bulone, Vincent; Tuskan, Gerald A; Heath, Katy; Zee, Francis; Moore, Paul H; Sunkar, Ramanjulu; Leebens-Mack, James H; Mockler, Todd; Bennetzen, Jeffrey L; Freeling, Michael; Sankoff, David; Paterson, Andrew H; Zhu, Xinguang; Yang, Xiaohan; Smith, J Andrew C; Cushman, John C; Paull, Robert E; Yu, Qingyi

    2015-12-01

    Pineapple (Ananas comosus (L.) Merr.) is the most economically valuable crop possessing crassulacean acid metabolism (CAM), a photosynthetic carbon assimilation pathway with high water-use efficiency, and the second most important tropical fruit. We sequenced the genomes of pineapple varieties F153 and MD2 and a wild pineapple relative, Ananas bracteatus accession CB5. The pineapple genome has one fewer ancient whole-genome duplication event than sequenced grass genomes and a conserved karyotype with seven chromosomes from before the ρ duplication event. The pineapple lineage has transitioned from C3 photosynthesis to CAM, with CAM-related genes exhibiting a diel expression pattern in photosynthetic tissues. CAM pathway genes were enriched with cis-regulatory elements associated with the regulation of circadian clock genes, providing the first cis-regulatory link between CAM and circadian clock regulation. Pineapple CAM photosynthesis evolved by the reconfiguration of pathways in C3 plants, through the regulatory neofunctionalization of preexisting genes and not through the acquisition of neofunctionalized genes via whole-genome or tandem gene duplication.

  8. Aroma Volatile Compounds from Two Fresh Pineapple Varieties in China

    Science.gov (United States)

    Zheng, Liang-Yong; Sun, Guang-Ming; Liu, Yu-Ge; Lv, Ling-Ling; Yang, Wen-Xiu; Zhao, Wei-Feng; Wei, Chang-Bin

    2012-01-01

    Volatile compounds from two pineapples varieties (Tainong No.4 and No.6) were isolated by headspace solid phase microextraction (HS-SPME) and identified and quantified by gas chromatography-mass spectrometry (GC/MS). In the Tainong No. 4 and No. 6 pineapples, a total of 11 and 28 volatile compounds were identified according to their retention time on capillary columns and their mass spectra, and quantified with total concentrations of 1080.44 μg·kg−1 and 380.66 μg·kg−1 in the Tainong No.4 and No. 6 pineapples, respectively. The odor active values (OAVs) of volatile compounds from pineapples were also calculated. According to the OAVs, four compounds were defined as the characteristic aroma compounds for the Tainong No. 4 pineapple, including furaneol, 3-(methylthio)propanoic acid methyl ester, 3-(methylthio)propanoic acid ethyl ester and δ-octalactone. The OAVs of five compounds including ethyl-2-methylbutyrate, methyl-2-methylbutyrate, 3-(methylthio)propanoic acid ethyl ester, ethyl hexanoate and decanal were considered to be the characteristic aroma compounds for the Tainong No. 6 pineapple. PMID:22837701

  9. Optimisation of microwave-assisted processing in production of pineapple jam

    Science.gov (United States)

    Ismail, Nur Aisyah Mohd; Abdullah, Norazlin; Muhammad, Norhayati

    2017-10-01

    Pineapples are available all year round since they are unseasonal fruits. Due to the continuous harvesting of the fruit, the retailers and farmers had to find a solution such as the processing of pineapple into jam, to treat the unsuccessfully sold pineapples. The direct heating of pineapple puree during the production of pineapple jam can cause over degradation of quality of the fresh pineapple. Thus, this study aims to optimise the microwave-assisted processing conditions for producing pineapple jam which could reduce water activity and meets minimum requirement for pH and total soluble solids contents of fruit jam. The power and time of the microwave processing were chosen as the factors, while the water activity, pH and total soluble solids (TSS) content of the pineapple jam were determined as responses to be optimised. The microwave treatment on the pineapple jam was able to give significant effect on the water activity and TSS content of the pineapple jam. The optimum power and time for the microwave processing of pineapple jam is 800 Watt and 8 minutes, respectively. The use of domestic microwave oven for the pineapple jam production results in acceptable pineapple jam same as conventional fruit jam sold in the marketplace.

  10. Propagation of pineapple by shoot tip culture

    International Nuclear Information System (INIS)

    Almobasher, H. A. A.; Osman, M. G.; Said, A. E.

    2009-01-01

    This study was conducted with the objective of modifying the composition of MS medium for the clonal propagation of pineapple using shoot tips of Smooth Cayenne cultivar. Modification were made on various medium components. Results showed that both MS salts at the full or half strength were optimal, and there was on significant difference between them. Sucrose concentrations of 3% and 6% were better than other concentrations tested for growth and development of plant lets. The cultures responded positively to the increase of adenine sulfate and 80 mg/1 was the optimal. As for the additions of NAA and BA , alone or in combinations, the best results were recorded with the combination of NAA at 0.01 mg/1, and BA at 3.0 mg/1 where the largest number of shoots was obtained. Better explants performance was achieved on liquid medium with cotton support compared to solid medium. (Author)

  11. A comparison of tensile properties of polyester composites reinforced with pineapple leaf fiber and pineapple peduncle fiber

    Science.gov (United States)

    Juraidi, J. M.; Shuhairul, N.; Syed Azuan, S. A.; Intan Saffinaz Anuar, Noor

    2013-12-01

    Pineapple fiber which is rich in cellulose, relatively inexpensive, and abundantly available has the potential for polymer reinforcement. This research presents a study of the tensile properties of pineapple leaf fiber and pineapple peduncle fiber reinforced polyester composites. Composites were fabricated using leaf fiber and peduncle fiber with varying fiber length and fiber loading. Both fibers were mixed with polyester composites the various fiber volume fractions of 4, 8 and 12% and with three different fiber lengths of 10, 20 and 30 mm. The composites panels were fabricated using hand lay-out technique. The tensile test was carried out in accordance to ASTM D638. The result showed that pineapple peduncle fiber with 4% fiber volume fraction and fiber length of 30 mm give highest tensile properties. From the overall results, pineapple peduncle fiber shown the higher tensile properties compared to pineapple leaf fiber. It is found that by increasing the fiber volume fraction the tensile properties has significantly decreased but by increasing the fiber length, the tensile properties will be increased proportionally. Minitab software is used to perform the two-way ANOVA analysis to measure the significant. From the analysis done, there is a significant effect of fiber volume fraction and fiber length on the tensile properties.

  12. METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE ( Ananas comosus var. comosus )

    OpenAIRE

    WANDREILLA MOREIRA GARCIA; WILLIAN KRAUSE; DEJÂNIA VIEIRA DE ARAÚJO; ISANE VERA KARSBURG; RIVANILDO DALLACORT

    2017-01-01

    The objective of this work was to evaluate Fusarium guttiforme inoculation methods and genetic resistance of pineapple accessions. Thus, three experiments were conducted: pathogen inoculation of different leaf types ( B, D and F ) of pineapple (1), pathogen inoculation of pineapple cuttings and detached D leaves (2), and identification of resistance to fusariosis in 19 pineapple accessions (3) sampled in the State of Mato Grosso, Brazil. The cultivars Pérola (susceptible...

  13. Effect of physical damage and storage of pineapple fruits on their suitability for juice production

    NARCIS (Netherlands)

    Hounhouigan, M.H.; Linnemann, A.R.; Ingenbleek, P.T.M.; Soumanou, M.M.; Trijp, van H.C.M.; Boekel, van T.

    2014-01-01

    This study evaluated the suitability of physically damaged pineapples, variety “MD2,” that were stored for up to 9 days at 20C for the production of fresh pineapple juice. Fresh pineapples were bruised and cut in different ways. The study showed an interaction effect of the physical damage

  14. 75 FR 51978 - United States Standards for Grades of Pineapple Juice

    Science.gov (United States)

    2010-08-24

    ...-327] United States Standards for Grades of Pineapple Juice AGENCY: Agricultural Marketing Service, USDA. ACTION: Notice; correction. SUMMARY: The United States Standards for Grades of Pineapple Juice... e-mail [email protected] . Corrected copies of the U.S. Standards for Grades of Pineapple...

  15. Heterogeneity in pineapple fruit quality results from plant heterogeneity at flower induction

    NARCIS (Netherlands)

    Fassinou Hotegni, V.N.; Lommen, W.J.M.; Agbossou, E.K.; Struik, P.C.

    2014-01-01

    Heterogeneity in fruit quality constitutes a major constraint in agri-food chains. In this paper the sources of the heterogeneity in pineapple in the field were studied in four experiments in commercial pineapple fields. The aims were to determine (a) whether differences in pineapple fruit quality

  16. Modifikasi Mata Pisau Cincin pada Mesin Pengupas Kulit Nanas (Pineapple Peeler)

    OpenAIRE

    Nusantari, Swastika

    2015-01-01

    Pineapple is a tropical fruit which is quite unique. Beside it has sharp scales on its skin, a juicy texture and it has sharp eyes through the outer body. This research was held to modify and to expand the pineapple peeler in order to simplified pineapple peeling with ring blade so we could peel a pineapple well and to compare the result to the previous blade. As the name, this blade shaped like a ring and the cutting side was honed and was sharpened. The capacity of pineapple peeler using ri...

  17. Production and Quality Evaluation of Pineapple Fruit Wine

    Science.gov (United States)

    Qi, Ningli; Ma, Lina; Li, Liuji; Gong, Xiao; Ye, Jianzhi

    2017-12-01

    The fermentation process of pineapple fruit wine was studied. The juice was inoculated with 5% (v/v) active yeast and held at 20 °C for 7 days. Total sugar and pH decreased while the alcoholic strength increased with increasing length of fermentation. The fermented fruit wine contains 2.29 g/L total acid, 10.2 % (v/v) alcohol, 5.4 °Brix soluble solids, pH 3.52. Pineapple wine detected 68 kinds of aroma components, including 34 esters, 13 alcohols. The ester material accounted for 52.25% of the main aroma components. The quality and sensory evaluation results indicated that pineapple fruit wine belongs to a kind of low alcohol wine, so it is easy to be accepted by the public.

  18. The energetic characterization of pineapple crown leaves.

    Science.gov (United States)

    Braga, R M; Queiroga, T S; Calixto, G Q; Almeida, H N; Melo, D M A; Melo, M A F; Freitas, J C O; Curbelo, F D S

    2015-12-01

    Energetic characterization of biomass allows for assessing its energy potential for application in different conversion processes into energy. The objective of this study is to physicochemically characterize pineapple crown leaves (PC) for their application in energy conversion processes. PC was characterized according to ASTM E871-82, E1755-01, and E873-82 for determination of moisture, ash, and volatile matter, respectively; the fixed carbon was calculated by difference. Higher heating value was determined by ASTM E711-87 and ash chemical composition was determined by XRF. The thermogravimetric and FTIR analyses were performed to evaluate the thermal decomposition and identify the main functional groups of biomass. PC has potential for application in thermochemical processes, showing high volatile matter (89.5%), bulk density (420.8 kg/m(3)), and higher heating value (18.9 MJ/kg). The results show its energy potential justifying application of this agricultural waste into energy conversion processes, implementing sustainability in the production, and reducing the environmental liabilities caused by its disposal.

  19. Towards Sustainable Use of Potassium in Pineapple Waste

    Directory of Open Access Journals (Sweden)

    Osumanu H. Ahmed

    2004-01-01

    Full Text Available Due to the 1997/98 haze problem in South-East Asia and the increasing need for sustainable food production and development, the usual management of crop residues (including pineapple wastes through burning is prohibited. As a result, the need for alternative uses of pineapple wastes in pineapple production has been emphasized. This study investigated an environmentally friendly means of recycling pineapple leaves for agricultural use. Pineapple leaves were shredded and composted in a composting drum for 30 days. Part of the shredded leaves was ashed in a muffle furnace for 4 h. Humic acid (HA, K-fulvate, and K in HA and compost were analyzed using standard procedures. An ash to water ratio of 1:7 was used to extract 0.1 molar (M KOH from the shredded leaves. The 0.1 M KOH contained 50% K and was able to extract 20% HA from the composted pineapple leaves. Percent K in the fulvate using 0.1 M KOH was 43. Besides serving as a foliar spray (supplement soil application K fertilizers, source of K for freshwater fish (e.g., tilapia, the HA produced can be used as a soil conditioner. Studies show that between 0.05–0–01 g of HA per kg soil retards runoff by 36% in sandy and sandy loam soils. The K-fulvate can be used as a fluid fertilizer. In addition, the pH of 2 of the K-fulvate suggests it could be used to dissolve phosphate rocks, particularly those in the arid regions where high soil pH does not facilitate the dissolution of these important rocks that serve as one of the sources of phosphorus fertilizer in agriculture.

  20. Towards sustainable use of potassium in pineapple waste.

    Science.gov (United States)

    Ahmed, Osumanu H; Husni, M H A; Anuar, A R; Hanafi, M M

    2004-11-20

    Due to the 1997/98 haze problem in South-East Asia and the increasing need for sustainable food production and development, the usual management of crop residues (including pineapple wastes) through burning is prohibited. As a result, the need for alternative uses of pineapple wastes in pineapple production has been emphasized. This study investigated an environmentally friendly means of recycling pineapple leaves for agricultural use. Pineapple leaves were shredded and composted in a composting drum for 30 days. Part of the shredded leaves was ashed in a muffle furnace for 4 h. Humic acid (HA), K-fulvate, and K in HA and compost were analyzed using standard procedures. An ash to water ratio of 1:7 was used to extract 0.1 molar (M) KOH from the shredded leaves. The 0.1 M KOH contained 50% K and was able to extract 20% HA from the composted pineapple leaves. Percent K in the fulvate using 0.1 M KOH was 43. Besides serving as a foliar spray (supplement soil application K fertilizers), source of K for freshwater fish (e.g., tilapia), the HA produced can be used as a soil conditioner. Studies show that between 0.05-0.01 g of HA per kg soil retards runoff by 36% in sandy and sandy loam soils. The K-fulvate can be used as a fluid fertilizer. In addition, the pH of 2 of the K-fulvate suggests it could be used to dissolve phosphate rocks, particularly those in the arid regions where high soil pH does not facilitate the dissolution of these important rocks that serve as one of the sources of phosphorus fertilizer in agriculture.

  1. Rancang Bangun Mesin Pengupas Kulit Nanas (Pineapple Peeler)

    OpenAIRE

    Noersalim, Yuli

    2015-01-01

    During this time,peeling pineapple fruit is known only through manual tools by kitchen knife. However, lately has begun to be developed in the form of the pineapple peeler toolwith manually press by using human operators power. With the development of science and technology (IPTEK) in this modern age, as human beings that have the potential to think will always develop it and trying to create or make a new one, more efficient equipment and practical that can help even replacing human power w...

  2. Effect of boron on fruit quality in pineapple

    Science.gov (United States)

    Wei, Changbin; Ma, Zhiling; Liu, Yuge; Qiao, Jian; Sun, Guangming

    2018-04-01

    Boron (B) is an important element for the plant. The aim of work was to study the effect of B on fruit quality of pineapple. The experiment was carried out with `Comte de paris' variety in pots. The results demonstrated that B had positive effect on fruit weight, TSS, the ratio of TSS/acidity, Vitamin C, the content of aroma volatile compounds. The B had no effect on the content of the three sugars (sucrose, fructose and glucose) and titrable acidity. There was a positive effect on fruit quality by application of B fertilizer in production of pineapple.

  3. Natural Pineapple Leaf Fibre Extraction On Josapine And Morris

    OpenAIRE

    Mazalan Muhammad Firdaus; Yusof Yusri

    2017-01-01

    The pineapple’s leaf plant contains approximately 2.5% to 3.5% of strong white silky fibres. These fibres are useful and can be extracted from the leaves. There are a few ways to extract the fibre such as hand scrapping and by extraction machine. The objective of this research is to study the quality of fibre extraction by using different age of pineapple leaf. Next, the study aims to compare the quality of Josapine and Morris pineapple leaf with tensile test. Fibre yield percentage are calcu...

  4. Growth, flowering and fruiting in vitro pineapple ( Ananas comosus L ...

    African Journals Online (AJOL)

    The experiment was carried out in a greenhouse, located in Tehran city, Iran. The objectives of this study was to evaluate the effects of fertilizer and acidic soil on the foliar and radicular growth of micropropagated plantlets of the pineapple cv. Merr (Ananas comosus L.). We evaluated the growth of that genotype in five ...

  5. Microbial and heavy metal contamination of pineapple products ...

    African Journals Online (AJOL)

    SAM

    3Department of Social Sciences, University of Rwanda, P.O. Box 117 Butare, ... pineapple processing Enterprises (SMEs) over a storage duration of 12 months. .... The results were measured against ... analyzed for microbial contamination using International Organization ... All culture media used were manufactured by.

  6. Alkali and bleach treatment of the extracted cellulose from pineapple ...

    African Journals Online (AJOL)

    (FTIR) spectroscopy, Thermogravimetric analysis (TGA) and Differential thermal analysis (DTA). SEM micrographs revealed that alkali treatment removed the impurities in the pineapple leaf fibers and subsequent bleaching further purify the fibers leaving mostly cellulose only while hemicellulose and lignin are removed as ...

  7. The Sliced Pineapple Grid Feature for Predicting Grasping Affordances

    DEFF Research Database (Denmark)

    Thomsen, Mikkel Tang; Kraft, Dirk; Krüger, Norbert

    2017-01-01

    The problem of grasping unknown objects utilising vision is addressed in this work by introducing a novel feature, the Sliced Pineapple Grid Feature (SPGF). The SPGF encode semi-local surfaces and allows for distinguishing structures such as “walls”,“edges” and “rims”. These structures are shown...

  8. The Analgesic Effect of Pineapple Fruit Juice on Mice

    Directory of Open Access Journals (Sweden)

    Ainul Atiqah binti Hilmi

    2014-08-01

    Full Text Available Background: Pain is a feeling stimulated by the nervous system which can be suppressed by giving an analgesic agent. Some studies revealed that pineapples have an analgesic effect. This study aim was to determine analgesic effect of pineapple on mice. Methods: In this experimental study, the effect was examined by using a writhing method on the 28 male mice. Subjects were divided into 4 groups with 7 mice each. The control group received aquades and other groups received pineapple fruit juice with 20%, 40% and 80% concentration with the dosage of 10 mL/kg/body weight. After 30 minutes, 3% acetic acid was injected intraperitoneally to induce pain. Writhing responseswere observed every 5 minutes for 30 minutes. Results: The result for mean of total writhing reaction was 2.39±0.40, 1.92±0.40, 1.50±2.13, 1.66±0.11 respectively for group 1 to 4. These data indicated a significant decrease of total writhing response in mice with 20%, 40% and 80% concentration compared to control group (p=0.023;p=0.000 and p=0.000 respectively. Most optimal concentration was40% with the protective percentage equal to 71.8%. Conclusion: Pineapple fruit juice concentrations (20%, 40%, and 80%has an analgesic effect with the most optimal concentration of 40%.

  9. Systemic allergic reaction and diarrhoea after pineapple ingestion

    NARCIS (Netherlands)

    Kabir, I.; Speelman, P.; Islam, A.

    1993-01-01

    Some foods may initiate allergic reactions. Anaphylaxis due to mangoes, oranges, nuts and other foods has been reported earlier. We report the clinical and laboratory features of 32 patients who became symptomatic shortly after they had eaten pineapples. Seventeen patients were males and 15 females

  10. Understanding the effects of slip pruning on pineapple fruit quality

    NARCIS (Netherlands)

    Fassinou Hotegni, V.N.; Lommen, W.J.M.; Agbossou, E.K.; Struik, P.C.

    2016-01-01

    Pineapple fruit quality is important especially when fruits are exported to international markets. Fruits should meet minimum requirements such as a weight of at least 0.7 kg, a ratio between the crown length and infructescence (fruit without the crown) length ranging from 0.5 to 1.5, and a Brix

  11. Pineapple Waste Extract for Preventing Oxidation in Model Food Systems.

    Science.gov (United States)

    Segovia Gómez, Francisco; Almajano Pablos, María Pilar

    2016-07-01

    Pineapple (Ananas comosus) is consumed in the form of chunks (canned), cubes, fruit salad, and also in juices, concentrates, and jams. In the processes to produce these products, the waste generated represents a high percentage of the total fruit. Some studies have shown that residues of certain fruits, such as pineapple, have the same antioxidant activity as the fruit pulp. So although these residues are discarded, they could be used as an alternative source of polyphenols, as natural antioxidants. This study is focused on the antioxidant activity of wastes obtained in the production of pineapple products and their application. The polyphenols' scavenging activity was determined by the oxygen radical antioxidant capacity assay. The antioxidant potential was determined in emulsions (o/w) and in muffins, where the primary oxidation products (by peroxide value, PV) and the secondary oxidation products (by thiobarbituric acid reactive substances) were analyzed. In addition the muffins were analyzed by means of a triangular sensory test. The PV method showed that pineapple waste extracts caused a reduction in oxidation products of 59% in emulsions and 91% in the muffins. The reduction in TBARs values for emulsions were 27% and for muffins were 51%. The triangular sensory test showed that the samples containing the extract were not distinguished from the control (α = 0.05). © 2016 Institute of Food Technologists®

  12. Thin Layer Drying Kinetics of Pineapple: Effect of Blanching ...

    African Journals Online (AJOL)

    Four thin-layer drying models were fitted to the experimental drying data. The .... MATLAB software package (version 6.5). The correlation ... to evaluate the goodness of fit of the simulation ... during the oven-drying process of pineapple slices.

  13. Mass micropropagation of pineapple tissue culture using bioreactor technology

    International Nuclear Information System (INIS)

    Irwan Syafri; Amir Hamzah Harun; Rusli Ibrahim

    2005-01-01

    Pineapple (ananas comosus) is the most important fruit in terms of revenue earner in this country. The export of the canned pineapple is about 2 million standard cases annually valued at RM 60 million, while the export of fresh pineapple is about 40,000 tonnes worth about RM 10 million. The industry for canning is however, an ailing industry with production on the decline since the 70s. Scaling up the pineapple propagation using in vitro methods seems to be possible solutions for the lack of planting material. Temporary immersion system (TIS) has been described by Teisson and Alvard (1995) for plant tissue culture propagation. This system, also known as RITA, has been successfully used with embryogenic tissues of banana (Alvard et al 1993), coffee (Berthouly 1991), rubber (Etienne et al 1993) and sugarcane (Lorenzo et al 1998). In this study, the system has been set up with a potential capacity of 3 manifolds with 10 RITA each, to multiply meristem explants at different immersion periods. The system was compared with the conventional micropropagation system on solid medium. Both systems were treated with MS media containing 2.5 mg/l BAP and 0.1 NAA. In TIS the shoots were able to multiplied faster in comparison with solid media. The multiplication rates were increased up to 1:3 to 1:5 compared to normal propagation on solid media. The results show that TIS not only increase the propagation rates of pineapple but could also be adapted to reduce implementation costs to establish low-cost propagation systems. (Author)

  14. Modeling the Pineapple Express phenomenon via Multivariate Extreme Value Theory

    Science.gov (United States)

    Weller, G.; Cooley, D. S.

    2011-12-01

    The pineapple express (PE) phenomenon is responsible for producing extreme winter precipitation events in the coastal and mountainous regions of the western United States. Because the PE phenomenon is also associated with warm temperatures, the heavy precipitation and associated snowmelt can cause destructive flooding. In order to study impacts, it is important that regional climate models from NARCCAP are able to reproduce extreme precipitation events produced by PE. We define a daily precipitation quantity which captures the spatial extent and intensity of precipitation events produced by the PE phenomenon. We then use statistical extreme value theory to model the tail dependence of this quantity as seen in an observational data set and each of the six NARCCAP regional models driven by NCEP reanalysis. We find that most NCEP-driven NARCCAP models do exhibit tail dependence between daily model output and observations. Furthermore, we find that not all extreme precipitation events are pineapple express events, as identified by Dettinger et al. (2011). The synoptic-scale atmospheric processes that drive extreme precipitation events produced by PE have only recently begun to be examined. Much of the current work has focused on pattern recognition, rather than quantitative analysis. We use daily mean sea-level pressure (MSLP) fields from NCEP to develop a "pineapple express index" for extreme precipitation, which exhibits tail dependence with our observed precipitation quantity for pineapple express events. We build a statistical model that connects daily precipitation output from the WRFG model, daily MSLP fields from NCEP, and daily observed precipitation in the western US. Finally, we use this model to simulate future observed precipitation based on WRFG output driven by the CCSM model, and our pineapple express index derived from future CCSM output. Our aim is to use this model to develop a better understanding of the frequency and intensity of extreme

  15. Development of Pineapple Microsatellite Markers and Germplasm Genetic Diversity Analysis

    Directory of Open Access Journals (Sweden)

    Suping Feng

    2013-01-01

    Full Text Available Two methods were used to develop pineapple microsatellite markers. Genomic library-based SSR development: using selectively amplified microsatellite assay, 86 sequences were generated from pineapple genomic library. 91 (96.8% of the 94 Simple Sequence Repeat (SSR loci were dinucleotide repeats (39 AC/GT repeats and 52 GA/TC repeats, accounting for 42.9% and 57.1%, resp., and the other three were mononucleotide repeats. Thirty-six pairs of SSR primers were designed; 24 of them generated clear bands of expected sizes, and 13 of them showed polymorphism. EST-based SSR development: 5659 pineapple EST sequences obtained from NCBI were analyzed; among 1397 nonredundant EST sequences, 843 were found containing 1110 SSR loci (217 of them contained more than one SSR locus. Frequency of SSRs in pineapple EST sequences is 1SSR/3.73 kb, and 44 types were found. Mononucleotide, dinucleotide, and trinucleotide repeats dominate, accounting for 95.6% in total. AG/CT and AGC/GCT were the dominant type of dinucleotide and trinucleotide repeats, accounting for 83.5% and 24.1%, respectively. Thirty pairs of primers were designed for each of randomly selected 30 sequences; 26 of them generated clear and reproducible bands, and 22 of them showed polymorphism. Eighteen pairs of primers obtained by the one or the other of the two methods above that showed polymorphism were selected to carry out germplasm genetic diversity analysis for 48 breeds of pineapple; similarity coefficients of these breeds were between 0.59 and 1.00, and they can be divided into four groups accordingly. Amplification products of five SSR markers were extracted and sequenced, corresponding repeat loci were found and locus mutations are mainly in copy number of repeats and base mutations in the flanking region.

  16. Development of Pineapple Microsatellite Markers and Germplasm Genetic Diversity Analysis

    Science.gov (United States)

    Tong, Helin; Chen, You; Wang, Jingyi; Chen, Yeyuan; Sun, Guangming; He, Junhu; Wu, Yaoting

    2013-01-01

    Two methods were used to develop pineapple microsatellite markers. Genomic library-based SSR development: using selectively amplified microsatellite assay, 86 sequences were generated from pineapple genomic library. 91 (96.8%) of the 94 Simple Sequence Repeat (SSR) loci were dinucleotide repeats (39 AC/GT repeats and 52 GA/TC repeats, accounting for 42.9% and 57.1%, resp.), and the other three were mononucleotide repeats. Thirty-six pairs of SSR primers were designed; 24 of them generated clear bands of expected sizes, and 13 of them showed polymorphism. EST-based SSR development: 5659 pineapple EST sequences obtained from NCBI were analyzed; among 1397 nonredundant EST sequences, 843 were found containing 1110 SSR loci (217 of them contained more than one SSR locus). Frequency of SSRs in pineapple EST sequences is 1SSR/3.73 kb, and 44 types were found. Mononucleotide, dinucleotide, and trinucleotide repeats dominate, accounting for 95.6% in total. AG/CT and AGC/GCT were the dominant type of dinucleotide and trinucleotide repeats, accounting for 83.5% and 24.1%, respectively. Thirty pairs of primers were designed for each of randomly selected 30 sequences; 26 of them generated clear and reproducible bands, and 22 of them showed polymorphism. Eighteen pairs of primers obtained by the one or the other of the two methods above that showed polymorphism were selected to carry out germplasm genetic diversity analysis for 48 breeds of pineapple; similarity coefficients of these breeds were between 0.59 and 1.00, and they can be divided into four groups accordingly. Amplification products of five SSR markers were extracted and sequenced, corresponding repeat loci were found and locus mutations are mainly in copy number of repeats and base mutations in the flanking region. PMID:24024187

  17. Improvement of pineapple using in vitro and mutation breeding techniques

    International Nuclear Information System (INIS)

    Lokko, Y.; Amoatey, H.

    2001-01-01

    Induction of genetic variation in pineapple (Ananas comosus (L.) Merr.), cv. 'Smooth Cayene' and 'Sugar Loaf, was investigated by irradiation of shoot tips with 45 Gy gamma rays. Irradiated shoot tips were sub-cultured in vitro, and 2500 plants of each cultivar were generated and acclimatised at 45/40 deg. C day/night temperatures. The plants were transplanted in field, and subjected to prolonged periods of drought and heat. In 'Smooth Cayene', the stress treatments did not produce any variant capable of survival under prolonged drought. In 'Sugar Loaf ', 54% of the plants, derived from irradiated shoot-tips, survived prolonged drought, but produced unmarketable fruits. A method of in vitro propagation to generate large numbers of pineapple plants was developed. (author)

  18. Mechanical properties of pineapple leaf fibre reinforced polypropylene composites

    International Nuclear Information System (INIS)

    Arib, R.M.N.; Sapuan, S.M.; Ahmad, M.M.H.M.; Paridah, M.T.; Zaman, H.M.D. Khairul

    2006-01-01

    Pineapple leaf fibre, which is rich in cellulose, relative inexpensive and abundantly available has the potential for polymer-reinforced composite. The present study investigates the tensile and flexural behaviours of pineapple leaf fibre-polypropylene composites as a function of volume fraction. The tensile modulus and tensile strength of the composites were found to be increasing with fibre content in accordance with the rule of mixtures. The tensile modulus and tensile strength with a volume fraction 10.8% are 687.02 and 37.28 MPa, respectively. The flexural modulus gives higher value at 2.7% volume fraction. The flexural strength of the composites containing 5.4% volume fraction was found to be higher than that of pure polypropylene resin by 5.1%. Scanning electron microscopic studies were carried out to understand the fibre-matrix adhesion and fibre breakage

  19. Gamma radiation influences postharvest disease incidence of pineapple fruits

    International Nuclear Information System (INIS)

    Damayanti, M.; Sharma, G.J.; Kundu, S.C.

    1992-01-01

    The application of gamma radiation for improving the storage of pineapple fruits [Ananas comosus (L.) Merr. cv. Queen] has been studied in an attempt to reduce decay caused by fungal pathogens such as Ceratocystis paradoxa (Dade)-Moreau and Penicillium purpurogenum Stoll. Gamma radiation at 50, 75, 100, 150, and 250 Gy improved shelf life. The maximum tolerable dose was approximately 250 Gy. Fruits irradiated with up to 150 Gy and then stored at 25 to 28C maintained their texture better than did the controls. Radiation, particularly at doses 250 Gy, caused browning of the skin and softening of tissues. Browning increased with increasing radiation dose and storage duration. Excessively high doses promoted spoilage. Doses in the range of 50 to 250 Gy, in combination with storage at 11 to 13C, can be used to reduce postharvest losses in pineapple due to fungal diseases and senescence, thereby extending shelf life

  20. Mechanical properties of pineapple leaf fibre reinforced polypropylene composites

    Energy Technology Data Exchange (ETDEWEB)

    Arib, R.M.N. [Department of Mechanical and Manufacturing Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Sapuan, S.M. [Department of Mechanical and Manufacturing Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia)]. E-mail: sapuan@eng.upm.edu.my; Ahmad, M.M.H.M. [Department of Mechanical and Manufacturing Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Paridah, M.T. [Faculty of Forestry, Universiti Putra Malaysia, 43400 Serdang, Selangor (Malaysia); Zaman, H.M.D. Khairul [Radiation Processing Technology Division, Malaysian Institute for Nuclear Technology Research (MINT), Bangi 43000 Kajang, Selangor (Malaysia)

    2006-07-01

    Pineapple leaf fibre, which is rich in cellulose, relative inexpensive and abundantly available has the potential for polymer-reinforced composite. The present study investigates the tensile and flexural behaviours of pineapple leaf fibre-polypropylene composites as a function of volume fraction. The tensile modulus and tensile strength of the composites were found to be increasing with fibre content in accordance with the rule of mixtures. The tensile modulus and tensile strength with a volume fraction 10.8% are 687.02 and 37.28 MPa, respectively. The flexural modulus gives higher value at 2.7% volume fraction. The flexural strength of the composites containing 5.4% volume fraction was found to be higher than that of pure polypropylene resin by 5.1%. Scanning electron microscopic studies were carried out to understand the fibre-matrix adhesion and fibre breakage.

  1. Research of Uncertainty Reasoning in Pineapple Disease Identification System

    Science.gov (United States)

    Liu, Liqun; Fan, Haifeng

    In order to deal with the uncertainty of evidences mostly existing in pineapple disease identification system, a reasoning model based on evidence credibility factor was established. The uncertainty reasoning method is discussed,including: uncertain representation of knowledge, uncertain representation of rules, uncertain representation of multi-evidences and update of reasoning rules. The reasoning can fully reflect the uncertainty in disease identification and reduce the influence of subjective factors on the accuracy of the system.

  2. SENSORY CHARACTERISTICS OF NATIVE CHICKEN QUEEN PINEAPPLE-CURED HAM

    OpenAIRE

    Dr. Lilibeth A. Roxas; Nikko A. Roxas

    2015-01-01

    The potential of Native Chicken to be processed into palatable ham was conducted making use of Queen Pineapple (QP) crude extract as one of the curing ingredients. Primarily, the main goal is to develop a protocol in the manufacture of processed native chicken ham and determine the organoleptic quality of native chicken ham product. The age of the bird and maturity of the fruit were considered for the best organoleptic quality of chicken ham. In this study, the combine injectio...

  3. MARKET RESEARCH, ELABORATION AND CHARACTERIZATION OF PINEAPPLE LIQUEUR

    Directory of Open Access Journals (Sweden)

    Vania Zanella Pinto

    2017-12-01

    Full Text Available Native trees and fruit have great importance in culture of small centers and rural areas, as well as, homemade liqueurs. However, some strategies such as marketing research are necessary to help for products development or even modifications to existing products. The objective was to know the habits related to the consumption of alcoholic beverages and to evaluate the consumer's interest about fruit liqueurs, as well as to develop and standardize and characterize a pineapple liqueur, besides assessing the sensorial characteristics of the same. The most of the interviewees do not have the habit of consuming fruit liqueurs, with beer being the preferred alcoholic beverage. Interest in trying a fruit liqueur was almost unanimous among respondents demonstrating the existence of a niche market for fruit liqueur. Consumers testing had no significant difference between all attributes, but it was being observed well-acceptance for all formulations with scores ranging from 6.80 to 7.20 and different concentrations of pineapple do not interfere with the sensorial characteristics of the product. Thus, it was concluded that the production of pineapple liqueurs with lower levels of fruit (30% are accepted and contribute to the economy of production.

  4. Food taboo of taking pineapple and milk at a time

    Directory of Open Access Journals (Sweden)

    Md. Mahbubur Rahman

    2017-02-01

    Full Text Available The purpose of this study was to assess whether potential toxic interactions occur between the pineapple and milk following oral administration in rats by comparing clinical signs, hematological and biochemical parameters with the normal control and toxic standard groups. Pineapple and milk solutions were made by 1:1 (PMS1 and 2:1 (PMS2 ratio, administered 12 mL/kg body weight. Forty rats were equally divided into 4 groups treated for 3 days: a normal control (only vehicle treated; b toxic standard (CCl4 was suspended in corn oil, 20% v/v; treated 1.25 mL/kg, c PMS1 and d PMS2 groups. CCl4 administration altered the normal behavior, changes gross and microscopic morphology. Toxicity related hematological and serum biochemistry changed significantly (p<0.05 than the normal group. However, all these clinical and pathological changes were completely absent in PMS treated groups. These results suggest that taking pineapple with milk is not toxic and this food taboo is wrong.

  5. Natural Pineapple Leaf Fibre Extraction On Josapine And Morris

    Directory of Open Access Journals (Sweden)

    Mazalan Muhammad Firdaus

    2017-01-01

    Full Text Available The pineapple’s leaf plant contains approximately 2.5% to 3.5% of strong white silky fibres. These fibres are useful and can be extracted from the leaves. There are a few ways to extract the fibre such as hand scrapping and by extraction machine. The objective of this research is to study the quality of fibre extraction by using different age of pineapple leaf. Next, the study aims to compare the quality of Josapine and Morris pineapple leaf with tensile test. Fibre yield percentage are calculated to determine which type of pineapple leaf produce high production of dry fibre. The mechanical properties of the fibres are analysed by Tensile Test under American Standard Testing Methods (ASTM C1577-03 and Scanning Electron Microscope (SEM. The result of the fibre yield percentage show the Josapine type on 12 month ages are the highest value fibre yield percentage which is 7.89%. Based on fibre yield percentages, it showed the Josapine type produce better dry fibre production compare to Morris type. Based on mechanical test, it showed Josapine type on 12 months ages are the strongest fibre compare to Morris type since it can withstand on 67.6 N of load.

  6. Pineapple peel wastes as a potential source of antioxidant compounds

    Science.gov (United States)

    Saraswaty, V.; Risdian, C.; Primadona, I.; Andriyani, R.; Andayani, D. G. S.; Mozef, T.

    2017-03-01

    Indonesia is a large pineapple (Ananas comosus) producing country. Food industries in Indonesia processed this fruit for new products and further resulted wastes of which cause an environmental problems. Approximately, one pineapple fruit total weight is 400 gr of which 60 g is of peel wastes. In order to reduce such pineapple peel wastes (PPW), processing to a valuable product using an environmentally friendly technique is indispensable. PPW contained phenolic compound, ferulic acid, and vitamin A and C as antioxidant. This study aimed to PPW using ethanol and water as well as to analyze its chemical properties. Both dried and fresh PPW were extracted using mixtures of ethanol and water with various concentrations ranging from 15 to 95% (v/v) at room temperature for 24 h. The chemical properties, such as antioxidant activity, total phenolic content (Gallic acid equivalent/GAE), and total sugar content were determined. The results showed that the range of Inhibition Concentration (IC)50 value as antioxidant activity of extracts from dried and fresh PPW were in the range of 0.8±0.05 to 1.3±0.09 mg.mL-1 and 0.25±0.01 to 0.59±0.01 mg.mL-1, respectively, with the highest antioxidant activity was in water extract. The highest of total phenolic content of 0.9 mg.g-1 GAE, was also found in water extract.

  7. Probing behavior of Dysmicoccus brevipes mealybug in pineapple plants

    Directory of Open Access Journals (Sweden)

    Lenira Viana Costa Santa-Cecília

    2016-12-01

    Full Text Available Differences in susceptibility to viruses in plants may be partially explained by the insect vector probing behavior and by the presence of phenolic compounds, which are often associated with defense strategies. This study aimed at detecting barriers that may difficult the probing activity of the Dysmicoccus brevipes (Pseudococcidae pineapple mealybug, a vector of the pineapple mealybug wilt-associated virus, as well as evaluating the phenolic content of plants, in order to verify any possible relationship with the probing behavior, by using the electrical penetration graphs (EPG technique. Seedlings of 'Smooth Cayenne' and 'Pérola' pineapple cultivars were used in the experiments. Only 28 % and 21 % of the mealybugs reached the phloem of the 'Smooth Cayenne' and 'Pérola' cultivars, respectively, over 16 h of recording, with an average of 9 h to reach the phloem vessels. The xylem phase was extended in both cultivars and represented approximately 31 % ('Smooth Cayenne' and 44 % ('Pérola' of the recording time. The phenolic contents of both cultivars were similar.

  8. Postharvest internal browning of pineapple fruit originates at the phloem.

    Science.gov (United States)

    Luengwilai, Kietsuda; Beckles, Diane M; Siriphanich, Jingtair

    2016-09-01

    A typical symptom of postharvest chilling injury (PCI) in pineapple fruit (Ananas comosus (L.) Merr.) is internal browning (IB) near the fruit core. Since vascular bundles (VBs) are localized to this region, it was hypothesized that the VBs might be the site of IB. To test this, the anatomy and histochemistry of VBs during chilling stress in four pineapple cultivars with different levels of sensitivity to PCI were examined. Fruit were stored at 10°C for up to three weeks to stimulate translucency symptoms (TS; the initiation of IB). After three weeks of chilling exposure, the cultivars 'MD2' showed 0%, 'Pattavia' and 'Savee' showed 10-16%, and 'Trad Sri Thong' showed 100% TS and IB symptom. Scanning electron microscopy and in situ histochemical staining techniques that detect enzymes and substrates commonly associated with IB initiation were used in parallel. The TS of pineapple fruit coincided with the collapse of the phloem tissue. The VBs in the tissue where IB was initiated (i.e., the flesh adjacent to the core or F/C) had the highest activity of polyphenol oxidase, hydrogen peroxide, and phenolic compounds. The IB-resistant 'MD2' genotype had fewer VBs, but a greater proportion of sclerenchyma fibers (Ppineapple IB occurrence in the phloem was proposed. Copyright © 2016 Elsevier GmbH. All rights reserved.

  9. Biogas production from pineapple core - A preliminary study

    Science.gov (United States)

    Jehan, O. S.; Sanusi, S. N. A.; Sukor, M. Z.; Noraini, M.; Buddin, M. M. H. S.; Hamid, K. H. K.

    2017-09-01

    Anaerobic digestion of pineapple waste was investigated by using pineapple core as the sole substrate. Pineapple core was chosen due to its high total sugar content thus, indicating high amount of fermentable sugar. As digestion process requires the involvement of microorganisms, wastewater from the same industry was added in the current study at ratio of 1:1 by weight. Two different sources of wastewater (Point 1 and Point 2) were used in this study to distinguish the performance of microorganism consortia in both samples. The experiment was conducted by using a lab scale batch anaerobic digester made up from 5L container with separate gas collecting system. The biogas produced was collected by using water displacement method. The experiment was conducted for 30 days and the biogas produced was collected and its volume was recorded at 3 days interval. Based on the data available, wastewater from the first point recorded higher volume of biogas with the total accumulated biogas volume is 216.1 mL. Meanwhile, wastewater sample from Point 2 produced a total of 140.5 mL of biogas, by volume. The data shows that the origin and type of microorganism undeniably play significant role in biogas production. In fact, other factors; pH of wastewater and temperature were also known to affect biogas production. The anaerobic digestion is seen as the promising and sustainable alternatives to current disposal method.

  10. Natural Endophytic Occurrence of Acetobacter diazotrophicus in Pineapple Plants.

    Science.gov (United States)

    Tapia-Hernández; Bustillos-Cristales; Jiménez-Salgado; Caballero-Mellado; Fuentes-Ramírez

    2000-01-01

    The presence of endophytic Acetobacter diazotrophicus was tested for pineapple plants (Ananas comosus [L.] Merr.) grown in the field. Diazotrophic bacteria were isolated from the inner tissues of surface sterilized roots, stems, and leaves of pineapple plants. Phenotypic tests permitted the selection of presumptive nitrogen-fixing A. diazotrophicus isolates. Restriction fragment length polymorphisms (RFLPs) of small subunit (SSU) rDNA using total DNA digested with endonuclease SphI and with endonuclease NcoI, hybridizations of RNA with an A. diazotrophicus large subunit (LSU) rRNA specific probe, as well as patterns in denaturing protein electrophoresis (SDS-PAGE) and multilocus enzyme tests allowed the identification of A. diazotrophicus isolates. High frequencies of isolation were obtained from propagative buds that had not been nitrogen-fertilized, and lower frequencies from 3-month-old plants that had been nitrogen-fertilized. No isolates were recovered from 5- to 7-month-old nitrogen-fertilized plants. All the A. diazotrophicus isolates recovered from pineapple plants belonged to the multilocus genotype which shows the most extensive distribution among all host species previously analyzed.

  11. Physicochemical, Proximate and Sensory Properties of Pineapple (Ananas sp.) Syrup Developed from Its Organic Side-Stream

    NARCIS (Netherlands)

    Tortoe, C.; Johnson, P.N.T.; Slaghek, T.; Miedema, M.; Timmermans, T.

    2013-01-01

    A major economical industrial challenge from pineapple (Ananas sp.) processing contributing to environmental pollu- tion is the organic side-streams of pineapple. The physicochemical, proximate and sensory properties of organic side- stream pineapple syrup (OSPS) developed from Smooth cayenne, Sugar

  12. The addition of pineapple flesh and pineapple peels extracts to increase the quality of used cooking oil

    Science.gov (United States)

    Kusumawardani, R.; Hasanah, N.; Sukemi

    2018-04-01

    In Indonesia, reuse of cooking oil is high and common. Heating process and reuse of the cooking oil causes a change in its chemical constituents and decrease its qualities. This research aimed to investigate the addition of pineapple flesh extract (PFE) and pineapple peel extract (PPE) on the increment of the quality of oxidized (used) cooking oil. The cooking oil has been used three times. Treatment was done by mixing the used cooking oil with the extract (2:1) at 50°C. Peroxide value, FFA and iodine number of treated and untreated used cooking oils were measured by using titration method. The result showed that the treatment could increase the quality of the used cooking oils. PPE was better than PFE to increase the quality of the used cooking oil.

  13. Microwave assisted air drying of osmotically treated pineapple with variable power programmes

    CSIR Research Space (South Africa)

    Botha, GE

    2012-01-01

    Full Text Available Variable power programmes for microwave assisted air drying of pineapple were studied. The pineapple pieces were pre-treated by osmotic dehydration in a 55º Brix sucrose solution at 40ºC for 90 minutes. Variable power output programmes were designed...

  14. Selective pruning in pineapple plants as means to reduce heterogeneity in fruit quality

    NARCIS (Netherlands)

    Fassinou Hotegni, V.N.; Lommen, W.J.M.; Struik, P.C.; Agbossou, E.K.

    2015-01-01

    Heterogeneity in fruit quality (size and taste) is a major problem in pineapple production chains. The possibilities were investigated of reducing the heterogeneity in pineapple in the field by pruning slips on selected plants, in order to promote the fruit growth on these plants. Slips are side

  15. Bottlenecks and opportunities for quality improvement in fresh pineapple supply chains in Benin

    NARCIS (Netherlands)

    Fassinou Hotegni, V.N.; Lommen, W.J.M.; Vorst, van der J.G.A.J.; Agbossou, E.K.; Struik, P.C.

    2014-01-01

    This study mapped and diagnosed the fresh pineapple supply chains in Benin to identify bottlenecks in pineapple quality improvement for different markets. A research framework was defined that comprised all relevant aspects to be researched. After 54 semi-structured interviews with key informants,

  16. First report of Pineapple mealybug wilt associated virus-1 in Ecuador

    Science.gov (United States)

    In Ecuador, where pineapple represents one of the most important export commodities, virus testing has been neglected. In July 2014, a total of twenty MD2 hybrid pineapple plants showing virus-like symptoms (Fig. 1) were collected from a commercial planting located at the border of Santo Domingo and...

  17. Quality of pasteurised pineapple juice in the context of the Beninese marketing system

    NARCIS (Netherlands)

    Hounhouigan, M.H.

    2014-01-01

    This study is a result of the interdisciplinary project Co-Innovation for Quality in African Food Chains (CoQA). The objective of the research was to improve the quality of pasteurised pineapple juice taking the characteristics of the Beninese pineapple marketing system into account. The specific

  18. inter-fertility among female parent clones of pineapple involved in a ...

    African Journals Online (AJOL)

    ACSS

    Ivoireconsiders fruit diversification as key component in the international pineapple industry. The objective of this study was to determine the sexual compatibility of female pineapple clones recently developed in Côte d'Ivoire. Three female hybrid clones, ...

  19. Effectiveness of radiation processing for elimination of Salmonella Typhimurium from minimally processed pineapple (Ananas comosus Merr.).

    Science.gov (United States)

    Shashidhar, Ravindranath; Dhokane, Varsha S; Hajare, Sachin N; Sharma, Arun; Bandekar, Jayant R

    2007-04-01

    The microbiological quality of market samples of minimally processed (MP) pineapple was examined. The effectiveness of radiation treatment in eliminating Salmonella Typhimurium from laboratory inoculated ready-to-eat pineapple slices was also studied. Microbiological quality of minimally processed pineapple samples from Mumbai market was poor; 8.8% of the samples were positive for Salmonella. D(10) (the radiation dose required to reduce bacterial population by 90%) value for S. Typhimurium inoculated in pineapple was 0.242 kGy. Inoculated pack studies in minimally processed pineapple showed that the treatment with a 2-kGy dose of gamma radiation could eliminate 5 log CFU/g of S. Typhimurium. The pathogen was not detected from radiation-processed samples up to 12 d during storage at 4 and 10 degrees C. The processing of market samples with 1 and 2 kGy was effective in improving the microbiological quality of these products.

  20. Effects of elevated temperature postharvest on color aspect, physiochemical characteristics, and aroma components of pineapple fruits.

    Science.gov (United States)

    Liu, Chuanhe; Liu, Yan

    2014-12-01

    In this work, 2 separate experiments were performed to describe the influence of elevated temperature treatments postharvest on the color, physiochemical characteristics and aroma components of pineapple fruits during low-temperature seasons. The L* (lightness) values of the skin and pulp of pineapple fruits were decreased. The a* (greenness-redness) and b* (blueness-yellowness) values of the skin and pulp were all markedly increased. The elevated temperature significantly increased the contents of total soluble solids (TSS) and slightly affected contents of vitamin C (nonsignificant). Titratable acidity (TA) of pineapple fruits were notably decreased, whereas the values of TSS/TA of pineapple fruits were significantly increased. The firmness of the pineapple fruits decreased and more esters and alkenes were identified. The total relative contents of esters were increased, and the total relative contents of alkenes were decreased. © 2014 Institute of Food Technologists®

  1. Developing single nucleotide polymorphism markers for the identification of pineapple (Ananas comosus) germplasm.

    Science.gov (United States)

    Zhou, Lin; Matsumoto, Tracie; Tan, Hua-Wei; Meinhardt, Lyndel W; Mischke, Sue; Wang, Boyi; Zhang, Dapeng

    2015-01-01

    Pineapple (Ananas comosus [L.] Merr.) is the third most important tropical fruit in the world after banana and mango. As a crop with vegetative propagation, genetic redundancy is a major challenge for efficient genebank management and in breeding. Using expressed sequence tag and nucleotide sequences from public databases, we developed 213 single nucleotide polymorphism (SNP) markers and validated 96 SNPs by genotyping the United States Department of Agriculture - Agricultural Research Service pineapple germplasm collection, maintained in Hilo, Hawaii. The validation resulted in designation of a set of 57 polymorphic SNP markers that revealed a high rate of duplicates in this pineapple collection. Twenty-four groups of duplicates were detected, encompassing 130 of the total 170 A cosmos accessions. The results show that somatic mutation has been the main source of intra-cultivar variations in pineapple. Multivariate clustering and a model-based population stratification suggest that the modern pineapple cultivars are comprised of progenies that are derived from different wild Ananas botanical varieties. Parentage analysis further revealed that both A. comosus var. bracteatus and A. comosus var. ananassoides are likely progenitors of pineapple cultivars. However, the traditional classification of cultivated pineapple into horticultural groups (e.g. 'Cayenne', 'Spanish', 'Queen') was not well supported by the present study. These SNP markers provide robust and universally comparable DNA fingerprints; thus, they can serve as an efficient genotyping tool to assist pineapple germplasm management, propagation of planting material, and pineapple cultivar protection. The high rate of genetic redundancy detected in this pineapple collection suggests the potential impact of applying this technology on other clonally propagated perennial crops.

  2. Optimizing the extraction of antibacterial compounds from pineapple leaf fiber

    Directory of Open Access Journals (Sweden)

    Zhuang Zhikai

    2016-01-01

    Full Text Available Five different solvents (petroleum ether, chloroform, ethyl acetate, acetone, and distilled water were used to extract antibacterial compounds from pineapple leaf fiber. Compounds extracted using acetone showed the greatest antibacterial effect against Escherichia coli, measured by inhibition zone diameter. Three extraction parameters including temperature, time and solid-liquid ratio were optimized through orthogonal experiment based on single factor investigations for achieving maximum active substance extraction rate and bacteriostatic effect. Results showed that using acetone, the optimum extraction conditions for temperature, time and solid-liquid ratio were 45°C, 8 h, and 1:40 (g/ml, respectively.

  3. The draft genome of MD-2 pineapple using hybrid error correction of long reads

    Science.gov (United States)

    Redwan, Raimi M.; Saidin, Akzam; Kumar, S. Vijay

    2016-01-01

    The introduction of the elite pineapple variety, MD-2, has caused a significant market shift in the pineapple industry. Better productivity, overall increased in fruit quality and taste, resilience to chilled storage and resistance to internal browning are among the key advantages of the MD-2 as compared with its previous predecessor, the Smooth Cayenne. Here, we present the genome sequence of the MD-2 pineapple (Ananas comosus (L.) Merr.) by using the hybrid sequencing technology from two highly reputable platforms, i.e. the PacBio long sequencing reads and the accurate Illumina short reads. Our draft genome achieved 99.6% genome coverage with 27,017 predicted protein-coding genes while 45.21% of the genome was identified as repetitive elements. Furthermore, differential expression of ripening RNASeq library of pineapple fruits revealed ethylene-related transcripts, believed to be involved in regulating the process of non-climacteric pineapple fruit ripening. The MD-2 pineapple draft genome serves as an example of how a complex heterozygous genome is amenable to whole genome sequencing by using a hybrid technology that is both economical and accurate. The genome will make genomic applications more feasible as a medium to understand complex biological processes specific to pineapple. PMID:27374615

  4. Cognitive enhancing of pineapple extract and juice in scopolamine-induced amnesia in mice

    Science.gov (United States)

    Momtazi-borojeni, Amir Abbas; Sadeghi-Aliabadi, Hojjat; Rabbani, Mohammed; Ghannadi, Alireza; Abdollahi, Elham

    2017-01-01

    The objective of the present study was to evaluate the cognitive enhancing of pineapple juice and ethanolic extract in scopolamine-induced cognitive deficit mice. The ethanolic extract of pineapple (Ananas comosus (L.) Merr.) was prepared by maceration method and its juice was obtained by a homogenizer. Object recognition task was used to evaluate the mice memory. Exploration time in the first and second trial was recorded. The differences in exploration time between a familiar and a novel object in the second trial were taken as a memory index. Animals were randomly assigned into 15 groups of 6 each including: control group (normal saline + vehicle), positive control group (scopolamine + rivastigmine), seven experimental groups (received scopolamine alone or scopolamine + ethanolic extract of pineapple in different doses), six other experimental groups were treated by ethanolic extract or juice of pineapple in different doses. Scopolamine (100 μL, 1 mg/kg, i.p.) and pineapple juice or extract (50, 75 and 100 mg/kg, i.p.) were administered 40 and 30 min before starting the second trial in the experimental groups. Object discrimination was impaired after scopolamine administration. Results showed that juice and ethanolic extract of pineapple significantly restored object recognition ability in mice treated with scopolamine. These finding suggested that pineapple had a protective role against scopolamine-induced amnesia, indicating its ability in management of cognitive disorders. PMID:28626484

  5. Transmission of Pineapple Mealybug Wilt-Associated Virus by Two Species of Mealybug (Dysmicoccus spp.).

    Science.gov (United States)

    Sether, D M; Ullman, D E; Hu, J S

    1998-11-01

    ABSTRACT Closterovirus-like particles associated with mealybug wilt of pineapple were acquired and transmitted by the pink pineapple mealybug, Dysmicoccus brevipes, and the gray pineapple mealybug, D. neobrevipes. Mealybugs acquired pineapple mealybug wilt-associated virus (PMWaV) from infected pineapple plants or detached leaves. The virus was detected in plants by tissue blot immunoassay and confirmed by immunosorbent electron microscopy. Plants exposed to mealybugs reared on PMWaV-free pineapple tissue remained uninfected. The presence of ants was correlated with an increased rate of virus spread when caged with D. brevipes. All stages of D. neobrevipes acquired PMWaV, although vector efficiency decreased significantly in older adult females. The probability of a single third-instar immature transmitting the virus was 0.04. Both species of mealybug acquired and transmitted PMWaV from infected pineapple material that had been clonally propagated for decades, and both species acquired PMWaV from sources previously infected with the virus by the other mealybug species.

  6. Acceptability of minimally processed and irradiated pineapple and watermelon among Brazilian consumers

    Energy Technology Data Exchange (ETDEWEB)

    Martins, Cecilia Geraldes; Aragon-Alegro, Lina Casale; Behrens, Jorge Herman; Oliveira Souza, Katia Leani [Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 580 B.14, 05508-900 Sao Paulo, SP (Brazil); Martins Vizeu, Dirceu; Hutzler, Beatriz Weltman [Embrarad Ltda. Av. Cruzada Bandeirante, 269, 06700-000 Cotia, SP (Brazil); Teresa Destro, Maria [Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 580 B.14, 05508-900 Sao Paulo, SP (Brazil); Landgraf, Mariza [Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 580 B.14, 05508-900 Sao Paulo, SP (Brazil)], E-mail: landgraf@usp.br

    2008-06-15

    This study aimed at evaluating the acceptance of MP watermelon and pineapple exposed to 1.0 and 2.5 kGy compared to non-irradiated samples. No significant differences were observed in liking between irradiated and non-irradiated samples, and also between doses of 1.0 and 2.5 kGy. Significant differences in sourness (pineapple) or sweetness (watermelon) and between intention of purchase of irradiated and non-irradiated fruits were not observed as well. Results showed that MP watermelon and pineapple could be irradiated with doses up to 2.5 kGy without significant changes in acceptability.

  7. Acceptability of minimally processed and irradiated pineapple and watermelon among Brazilian consumers

    International Nuclear Information System (INIS)

    Martins, Cecilia Geraldes; Aragon-Alegro, Lina Casale; Behrens, Jorge Herman; Oliveira Souza, Katia Leani; Martins Vizeu, Dirceu; Hutzler, Beatriz Weltman; Teresa Destro, Maria; Landgraf, Mariza

    2008-01-01

    This study aimed at evaluating the acceptance of MP watermelon and pineapple exposed to 1.0 and 2.5 kGy compared to non-irradiated samples. No significant differences were observed in liking between irradiated and non-irradiated samples, and also between doses of 1.0 and 2.5 kGy. Significant differences in sourness (pineapple) or sweetness (watermelon) and between intention of purchase of irradiated and non-irradiated fruits were not observed as well. Results showed that MP watermelon and pineapple could be irradiated with doses up to 2.5 kGy without significant changes in acceptability

  8. Acceptability of minimally processed and irradiated pineapple and watermelon among Brazilian consumers

    Science.gov (United States)

    Martins, Cecília Geraldes; Aragon-Alegro, Lina Casale; Behrens, Jorge Herman; Oliveira Souza, Kátia Leani; Martins Vizeu, Dirceu; Hutzler, Beatriz Weltman; Teresa Destro, Maria; Landgraf, Mariza

    2008-06-01

    This study aimed at evaluating the acceptance of MP watermelon and pineapple exposed to 1.0 and 2.5 kGy compared to non-irradiated samples. No significant differences were observed in liking between irradiated and non-irradiated samples, and also between doses of 1.0 and 2.5 kGy. Significant differences in sourness (pineapple) or sweetness (watermelon) and between intention of purchase of irradiated and non-irradiated fruits were not observed as well. Results showed that MP watermelon and pineapple could be irradiated with doses up to 2.5 kGy without significant changes in acceptability.

  9. An Electrochemical Impedance Spectroscopy System for Monitoring Pineapple Waste Saccharification

    Directory of Open Access Journals (Sweden)

    Claudia Conesa

    2016-02-01

    Full Text Available Electrochemical impedance spectroscopy (EIS has been used for monitoring the enzymatic pineapple waste hydrolysis process. The system employed consists of a device called Advanced Voltammetry, Impedance Spectroscopy & Potentiometry Analyzer (AVISPA equipped with a specific software application and a stainless steel double needle electrode. EIS measurements were conducted at different saccharification time intervals: 0, 0.75, 1.5, 6, 12 and 24 h. Partial least squares (PLS were used to model the relationship between the EIS measurements and the sugar determination by HPAEC-PAD. On the other hand, artificial neural networks: (multilayer feed forward architecture with quick propagation training algorithm and logistic-type transfer functions gave the best results as predictive models for glucose, fructose, sucrose and total sugars. Coefficients of determination (R2 and root mean square errors of prediction (RMSEP were determined as R2 > 0.944 and RMSEP < 1.782 for PLS and R2 > 0.973 and RMSEP < 0.486 for artificial neural networks (ANNs, respectively. Therefore, a combination of both an EIS-based technique and ANN models is suggested as a promising alternative to the traditional laboratory techniques for monitoring the pineapple waste saccharification step.

  10. Technological feasibility of irradiating pineapples for shelf-life extension

    International Nuclear Information System (INIS)

    Sharma, Jiten

    2001-01-01

    The spoilage of food caused by infestation, contamination and deterioration of the world's food supply is enormous in the developing countries where warm and humid climate favour the growth of spoilage organisms and hasten deterioration of stored food. Particularly, in a country like India where the population growth has already attained one billion, any preventable loss of food is intolerable. Since food security of a nation also largely determines the economic stability as well as self-reliance, this problem draws utmost attention not only of the food scientists but also of the food policy makers. From these above facts, it may be concluded that radiation technology can be safely used for extending shelf-life of pineapple fruits thereby maximizing the transportation and marketing potentials. Consumption of such irradiated pineapples simultaneously does not pose any immediate or long-term human health risks. Keeping these techno-economic considerations in view establishment of large scale industrial operations for irradiating fruits, vegetables, cereals etc. are of immense importance

  11. Processing of mixed fruit juice from mango, orange and pineapple

    Directory of Open Access Journals (Sweden)

    Sajeda Begam

    2018-08-01

    Full Text Available The study was conducted to prepare mixed fruit juice by using mango pulp, pineapple and orange juices and the quality in terms of nutritional value, keeping quality, shelf life and consumers’ acceptability were investigated. Chemical analysis showed that TSS, acidity were increased slightly whereas vitamin C and pH were decreased gradually during the storage periods. Storage studies were carried out up to one month with an interval of one week and the result showed that all the samples were in good condition after one month, though little bit of faded color was found at the end of storage periods. Sample with 35% mango juice, 40% orange juice and 25% pineapple secured the highest score on sensory evaluation and showed the best consumer acceptance. This research reveals that perishable fruits can be converted to attractive mixed juice and thus increase the shelf-life, which increase value of the product. [Fundam Appl Agric 2018; 3(2.000: 440-445

  12. Extraction and characterisation of cellulose nanocrystals from pineapple peel

    Directory of Open Access Journals (Sweden)

    Ana Raquel Madureira

    2018-04-01

    Full Text Available The potential of pineapple peel as a source of cellulose nanocrystals was evaluated. Peels skin from fresh-cut fruit was used as raw material. These residues were purified to remove pigments, lipids and hemicellulose, and a bleaching process for delignification was carried out for 4-6 h. All resulting products were characterised for their lignin, hemicellulose, cellulose and ash contents using standard techniques. Dry matter at the end was low (ca. 50% compared with the raw material (ca. 90%. The process applied resulted in ca. 20% (m/m of purified cellulose (ca. 80% purity, with ineligible levels of lignin and hemicellulose present, especially when using 6h of bleaching. The purified cellulose was subject to acid hydrolysis for nanocrystal extraction with two testing times, 30 and 60 minutes. These cellulose nanocrystals had small sizes (< 1000 nm, with high variability and negative zeta potential values. The time of extraction did not affect the nanocrystals’ chemical and physical properties. The use of 6 h of bleaching treatment during purification was shown to be more effective than 4 h. Pineapple peel was demonstrated to be a good source of cellulose for the production of cellulose nanocrystals.

  13. Acclimatization and growth of ornamental pineapple seedlings under organic substrates

    Directory of Open Access Journals (Sweden)

    Ronan Carlos Colombo

    2017-09-01

    Full Text Available The in vitro propagation techniques are commonly used to produce ornamental pineapple seedlings in commercial scale, aiming to attend the growers with genetic and sanitary quality seedlings. However, the choice of the ideal substrate is essential for the acclimatization and growth stage of the seedlings propagated by this technique, since some substrates can increase the seedling mortality and/or limit the seedling growth due to its physical and chemical characteristics. Thus, the aim of this study was to evaluate the acclimatization of ornamental pineapple [Ananas comosus (L. Merr. var. ananassoides (Baker Coppens & Leal] on different substrates. Seedlings with approximately seven centimeters, obtained from in vitro culture, were transplanted into styrofoam trays filled with the following substrates: sphagnum; semi-composed pine bark; carbonized rice husk; sphagnum + semicomposed pine bark; sphagnum + carbonized rice husk; and semi-composed pine bark + carbonized rice husk. Each treatment was replicated five times using 10 plants. At 180 days, there were evaluated the following variables: survival percentage, plant height, number of leaves, leaf area, largest root length, and shoot and root dry matter. The substrate semi-composed pine bark + carbonized rice husk presented the lowest mean (62% for survival percentage. The semi-composed pine bark and semi-composed pine bark + carbonized rice husk treatments presented significant increments in some evaluated biometric characteristics. The semi-composed pine bark is the most favorable substrate for the A. comosus var. ananassoids acclimatization.

  14. Correlations of Rotylenchulus reniformis Population Densities with 1,3-Dichloropropene Dosage Rate and Pineapple Yields

    OpenAIRE

    Schenck, Susan

    1990-01-01

    The relationships between Rotylenchulus reniformis population densities and pineapple growth and yield were studied in a small-plot field experiment. Increasing rates of handgun-injected 1,3-dichloropropene (1,3-D) preplant fumigant from 0 to 337 liters/ha resulted in greater nematode control, faster plant growth, and larger pineapple fruits. Rotylenchulus reniformis population densities at 2, 4, 6, and 8 months postplant were correlated with plant size and yield. The shorter the time period ...

  15. Biohydrogen Production from Pineapple Waste: Effect of Substrate Concentration and Acid Pretreatment

    Science.gov (United States)

    Cahyari, K.; Putri, A. M.; Oktaviani, E. D.; Hidayat, M. A.; Norajsha, J. D.

    2018-05-01

    Biohydrogen is the ultimate choice of energy carrier in future due to its superior qualities such as fewer greenhouse gases emission, high energy density (142 kJ/gram), and high energy conversion using a fuel cell. Production of biohydrogen from organic waste e.g. pineapple waste offers a simultaneous solution for renewable energy production and waste management. It is estimated that pineapple cultivation in Indonesia generated more than 1 million ton/year comprising of rotten pineapple fruit, leaves, and stems. Majority of this waste is dumped into landfill area without any treatments which lead to many environmental problems. This research was meant to investigate the utilization of pineapple waste i.e. peel and the core of pineapple fruit and leaves to produce biohydrogen through mesophilic dark fermentation (30°C, 1 atm, pH 5.0). Effect of dilute acid treatment and substrate concentration was particularly investigated in these experiments. Peel and core of pineapple waste were subjected to fermentation at 3 various substrate concentration i.e. 8.8, 17.6 and 26.4-gram VS/liter. Meanwhile, pineapple leaves were pretreated using dilute acid (H2SO4) at 0.2, 0.3 and 0.4 N and followed by dark fermentation. Results show that the highest yield of biohydrogen was obtained at a substrate concentration of 26.4-gram VS/liter both for peel and core of the waste. Pretreatment using dilute acid (H2SO4) 0.3 N might improve fermentation process with a higher yield at 0.8 ml/gram VS. Hydrogen percentage in biogas produced during fermentation process was in the range between 5 – 32% of volume ratio. In summary, it is possible to utilize pineapple waste for production of biohydrogen at an optimum substrate concentration of 26.4-gram VS/liter and acid pretreatment (H2SO4) of 0.3 N.

  16. Development and evaluation of a new method for sampling and monitoring the symphylid population in pineapple

    OpenAIRE

    Soler, A.; Gaude, J. M.; Marie-Alphonsine, P. A.; Vinatier, F.; Dole, B.; Govindin, J. C.; Fournier, P.; Quénéhervé, Patrick

    2011-01-01

    BACKGROUND: Symphylids (Hanseniella sp.) are polyphagous soilborne parasites. Today, symphylid populations on pineapple are monitored by observing root symptoms and the presence of symphylids at the bottom of basal leaves. The authors developed a reliable method with a bait and trap device to monitor symphylid populations in pineapple or fallow crops. The spatial distribution of the symphylid populations was evaluated using the variance/mean ratios and spatial analyses based on Moran's and Ge...

  17. Physicochemical Properties of Pineapple Plant Waste Fibers from the Leaves and Stems of Different Varieties

    OpenAIRE

    Muhammad Fakhri Zainuddin; Rosnah Shamsudin; Mohd Noriznan Mokhtar; Dahlan Ismail

    2014-01-01

    Pineapple agro-waste, the residue produced during harvesting or processing activities, is widely available around the world. After harvesting, most pineapple residue is disposed of and serves as fertilizer, or is burnt in an open field. However, these methods are not only ineffective, but also contribute to air pollution. The main objective of this study is to determine the physicochemical properties (i.e., cellulose, hemicellulose, lignin, proximate composition, dry matter, and nitrogen cont...

  18. Odor-active constituents in fresh pineapple (Ananas comosus [L.] Merr.) by quantitative and sensory evaluation.

    Science.gov (United States)

    Tokitomo, Yukiko; Steinhaus, Martin; Büttner, Andrea; Schieberle, Peter

    2005-07-01

    By application of aroma extract dilution analysis (AEDA) to an aroma distillate prepared from fresh pineapple using solvent-assisted flavor evaporation (SAFE), 29 odor-active compounds were detected in the flavor dilution (FD) factor range of 2 to 4,096. Quantitative measurements performed by stable isotope dilution assays (SIDA) and a calculation of odor activity values (OAVs) of 12 selected odorants revealed the following compounds as key odorants in fresh pineapple flavor: 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDF; sweet, pineapple-like, caramel-like), ethyl 2-methylpropanoate (fruity), ethyl 2-methylbutanoate (fruity) followed by methyl 2-methylbutanoate (fruity, apple-like) and 1-(E,Z)-3,5-undecatriene (fresh, pineapple-like). A mixture of these 12 odorants in concentrations equal to those in the fresh pineapple resulted in an odor profile similar to that of the fresh juice. Furthermore, the results of omission tests using the model mixture showed that HDF and ethyl 2-methylbutanoate are character impact odorants in fresh pineapple.

  19. New Insights for Diagnosis of Pineapple Fusariosis by MALDI-TOF MS Technique.

    Science.gov (United States)

    Santos, Cledir; Ventura, José Aires; Lima, Nelson

    2016-08-01

    Fusarium is one of the most economically important fungal genus, since it includes many pathogenic species which cause a wide range of plant diseases. Morphological or molecular biology identification of Fusarium species is a limiting step in the fast diagnosis and treatment of plant disease caused by these fungi. Mass spectrometry by matrix-assisted laser/desorption ionisation-time-of-flight (MALDI-TOF)-based fingerprinting approach was applied to the fungal growth monitoring and direct detection of strain Fusarium guttiforme E-480 inoculated in both pineapple cultivars Pérola and Imperial side shoots, that are susceptible and resistant, respectively, to this fungal strain. MALDI-TOF MS technique was capable to detect fungal molecular mass peaks in the susceptible pineapple stem side shoot tissue. It is assumed that these molecular masses are mainly constituted by ribosomal proteins. MALDI-TOF-based fingerprinting approach has herein been demonstrated to be sensitive and accurate for the direct detection of F. guttiforme E-480 molecular masses on both susceptible and resistant pineapple side stem free of any pre-treatment. According to the results obtained, the changing on molecular mass peaks of infected susceptible pineapple tissue together with the possibility of fungal molecular masses analysis into this pineapple tissue can be a good indication for an early diagnosis by MALDI-TOF MS of pineapple fusariosis.

  20. De novo assembly, characterization and functional annotation of pineapple fruit transcriptome through massively parallel sequencing.

    Science.gov (United States)

    Ong, Wen Dee; Voo, Lok-Yung Christopher; Kumar, Vijay Subbiah

    2012-01-01

    Pineapple (Ananas comosus var. comosus), is an important tropical non-climacteric fruit with high commercial potential. Understanding the mechanism and processes underlying fruit ripening would enable scientists to enhance the improvement of quality traits such as, flavor, texture, appearance and fruit sweetness. Although, the pineapple is an important fruit, there is insufficient transcriptomic or genomic information that is available in public databases. Application of high throughput transcriptome sequencing to profile the pineapple fruit transcripts is therefore needed. To facilitate this, we have performed transcriptome sequencing of ripe yellow pineapple fruit flesh using Illumina technology. About 4.7 millions Illumina paired-end reads were generated and assembled using the Velvet de novo assembler. The assembly produced 28,728 unique transcripts with a mean length of approximately 200 bp. Sequence similarity search against non-redundant NCBI database identified a total of 16,932 unique transcripts (58.93%) with significant hits. Out of these, 15,507 unique transcripts were assigned to gene ontology terms. Functional annotation against Kyoto Encyclopedia of Genes and Genomes pathway database identified 13,598 unique transcripts (47.33%) which were mapped to 126 pathways. The assembly revealed many transcripts that were previously unknown. The unique transcripts derived from this work have rapidly increased of the number of the pineapple fruit mRNA transcripts as it is now available in public databases. This information can be further utilized in gene expression, genomics and other functional genomics studies in pineapple.

  1. Fresh-Cut Pineapple as a New Carrier of Probiotic Lactic Acid Bacteria

    Science.gov (United States)

    Russo, Pasquale; de Chiara, Maria Lucia Valeria; Vernile, Anna; Amodio, Maria Luisa; Arena, Mattia Pia; Capozzi, Vittorio; Massa, Salvatore; Spano, Giuseppe

    2014-01-01

    Due to the increasing interest for healthy foods, the feasibility of using fresh-cut fruits to vehicle probiotic microorganisms is arising scientific interest. With this aim, the survival of probiotic lactic acid bacteria, belonging to Lactobacillus plantarum and Lactobacillus fermentum species, was monitored on artificially inoculated pineapple pieces throughout storage. The main nutritional, physicochemical, and sensorial parameters of minimally processed pineapples were monitored. Finally, probiotic Lactobacillus were further investigated for their antagonistic effect against Listeria monocytogenes and Escherichia coli O157:H7 on pineapple plugs. Our results show that at eight days of storage, the concentration of L. plantarum and L. fermentum on pineapples pieces ranged between 7.3 and 6.3 log cfu g−1, respectively, without affecting the final quality of the fresh-cut pineapple. The antagonistic assays indicated that L. plantarum was able to inhibit the growth of both pathogens, while L. fermentum was effective only against L. monocytogenes. This study suggests that both L. plantarum and L. fermentum could be successfully applied during processing of fresh-cut pineapples, contributing at the same time to inducing a protective effect against relevant foodborne pathogens. PMID:25093163

  2. Application of Pineapple Juice in the Fish Digestion Process for Carcinogenic Liver Fluke Metacercaria Collection

    Science.gov (United States)

    Sripan, Panupan; Aukkanimart, Ratchadawan; Boonmars, Thidarut; Pranee, Sriraj; Songsri, Jiraporn; Boueroy, Parichart; Khueangchaingkhwang, Sukhonthip; Pumhirunroj, Benjamabhorn; Artchayasawat, Atchara

    2017-01-01

    Pepsin is common digestive enzyme used for fish digestion in the laboratory to collect trematode metacercariae. In a field study, to survey the infected fish is needed a huge yield of pepsin and it is very expensive. Therefore, our purpose of this study was to investigate the candidate enzyme from pineapple juice which has a digestive enzyme called bromelain, a mixture of proteolytic enzymes, to digest fish in order to harvest metacercariae. Fish were divided into 2 groups: one group in which metacercariae were harvested using acid pepsin as a control and other groups in which the fish was digested using fresh pineapple juices. The results showed that pineapple juice is able to digest fish similarly to pepsin. The Pattavia pineapple juice had the highest number of metacercariae similar to the control. For Trat Si Thong pineapple juice, we found the number of metacercariae was less than control. This result suggests that the Pattavia pineapple juice was optimal juice for fish digestion to metacercaria collection and can be used instread of pepsin acid. PMID:28441786

  3. METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE ( Ananas comosus var. comosus

    Directory of Open Access Journals (Sweden)

    WANDREILLA MOREIRA GARCIA

    2017-01-01

    Full Text Available The objective of this work was to evaluate Fusarium guttiforme inoculation methods and genetic resistance of pineapple accessions. Thus, three experiments were conducted: pathogen inoculation of different leaf types ( B, D and F of pineapple (1, pathogen inoculation of pineapple cuttings and detached D leaves (2, and identification of resistance to fusariosis in 19 pineapple accessions (3 sampled in the State of Mato Grosso, Brazil. The cultivars Pérola (susceptible to fusariosis and BRS - Vitória (resistant to fusariosis were used as controls. The fusariosis severity was evaluated at 10, 15, 20, 25 and 30 days after inoculation with F. guttiforme . The lesion diameters (severity level were used in order to calculate the area under the disease progress curve (AUDPC. The inoculation of detached D leaves was the most efficient, fast and inexpensive method, and the one that most satisfactorily reproduced the disease symptoms. The period of 10 to 20 days after inoculation of the D detached leaves with the pathogen is the most suitable to evaluate the resistance of pineapple accessions to fusariosis. The lowest lesion area and AUDPC was found in the accession 1, in all evaluations. Thus, the accession 1 can be used in pineapple breeding programs for resistance to fusariosis.

  4. Dimensional stability of pineapple leaf fibre reinforced phenolic composites

    Science.gov (United States)

    Asim, M.; Jawaid, M.; Abdan, K.; Ishak, M. R.

    2017-12-01

    In this research, pineapple leaves fibre (PALF)/phenolic resin (PF) composites were fabricated by hand lay-up method. The aim of this work is to investigate the physical properties (water absorption and thickness swelling) of PALF reinforced phenolic resin composites. Long-term water absorption (WA) and thickness swelling (TS) behaviours of the PALF/PF composites were investigated at several water immersion times. The effects of different fibre loading on WA and TS of PALF/PF composites were also analyzed. Obtained results indicated that the WA and TS of PALF/PF composites vary with fibres content and water immersion time before reaching to equilibrium. WA and TS of PALF/PF composites were increased by increasing fibre loading. Results obtained in this study will be used for further study on hybridization of PALF and Kenaf fibre based phenolic composites.

  5. Physiological and enzymatic analyses of pineapple subjected to ionizing radiation

    International Nuclear Information System (INIS)

    Silva, Josenilda Maria da; Silva, Juliana Pizarro; Spoto, Marta Helena Fillet

    2007-01-01

    The physiological and enzymatic post-harvest characteristics of the pineapple cultivar Smooth Cayenne were evaluated after the fruits were gamma-irradiated with doses of 100 and 150 Gy and the fruits were stored for 10, 20 and 30 days at 12 deg C (±1) and relative humidity of 85% (±5). Physiological and enzymatic analyses were made for each storage period to evaluate the alterations resulting from the application of ionizing radiation. Control specimens showed higher values of soluble pectins, total pectins, reducing sugars, sucrose and total sugars and lower values of polyphenyloxidase and polygalacturonase enzyme activities. All the analyses indicated that storage time is a significantly influencing factor. The 100 Gy dosage and 20-day storage period presented the best results from the standpoint of maturation and conservation of the fruits quality. (author)

  6. A highly sensitive single-tube nested PCR assay for the detection of Pineapple mealybug wilt associated virus-2 (PMWaV-2)

    Science.gov (United States)

    An assay was developed for the detection of Pineapple mealybug wilt associated virus-2 (PMWaV-2), an important factor in the etiology of mealybug wilt of pineapple. The assay combines reverse transcription of RNA isolated from pineapple with a specific and very sensitive, single, closed-tube nested ...

  7. Aroma profile and volatiles odor activity along gold cultivar pineapple flesh.

    Science.gov (United States)

    Montero-Calderón, Marta; Rojas-Graü, María Alejandra; Martín-Belloso, Olga

    2010-01-01

    Physicochemical attributes, aroma profile, and odor contribution of pineapple flesh were studied for the top, middle, and bottom cross-sections cut along the central axis of Gold cultivar pineapple. Relationships between volatile and nonvolatile compounds were also studied. Aroma profile constituents were determined by headspace solid-phase microextraction at 30 °C, followed by gas chromatography/mass spectrometry analysis. A total of 20 volatile compounds were identified and quantified. Among them, esters were the major components which accounted for 90% of total extracted aroma. Methyl butanoate, methyl 2-methyl butanoate, and methyl hexanoate were the 3 most abundant components representing 74% of total volatiles in pineapple samples. Most odor active contributors were methyl and ethyl 2-methyl butanoate and 2,5-dimethyl 4-methoxy 3(2H)-furanone (mesifuran). Aroma profile components did not vary along the fruit, but volatile compounds content significantly varied (P fresh-cut pineapple trays, compromising consumer perception and acceptance of the product. Such finding highlighted the need to include volatiles content and SSC/TA ratio and their variability along the fruit as selection criteria for pineapples to be processed and quality assessment of the fresh-cut fruit. © 2010 Institute of Food Technologists®

  8. Cultivar identification and genetic relationship of pineapple (Ananas comosus) cultivars using SSR markers.

    Science.gov (United States)

    Lin, Y S; Kuan, C S; Weng, I S; Tsai, C C

    2015-11-25

    The genetic relationships among 27 pineapple [Ananas comosus (L.) Merr.] cultivars and lines were examined using 16 simple sequence repeat (SSR) markers. The number of alleles per locus of the SSR markers ranged from 2 to 6 (average 3.19), for a total of 51 alleles. Similarity coefficients were calculated on the basis of 51 amplified bands. A dendrogram was created according to the 16 SSR markers by the unweighted pair-group method. The banding patterns obtained from the SSR primers allowed most of the cultivars and lines to be distinguished, with the exception of vegetative clones. According to the dendrogram, the 27 pineapple cultivars and lines were clustered into three main clusters and four individual clusters. As expected, the dendrogram showed that derived cultivars and lines are closely related to their parental cultivars; the genetic relationships between pineapple cultivars agree with the genealogy of their breeding history. In addition, the analysis showed that there is no obvious correlation between SSR markers and morphological characters. In conclusion, SSR analysis is an efficient method for pineapple cultivar identification and can offer valuable informative characters to identify pineapple cultivars in Taiwan.

  9. Induced chlorophyll variation in pineapple c v. 'queen' by gamma irradiation (60Cobalt)

    International Nuclear Information System (INIS)

    Valencia, Lolita DC.

    2012-01-01

    Irradiation using gamma rays ( 60 Cobalt) coupled with in vitro culture techniques was undertaken to induce variation or mutation in pineapple. Calli from crown meristem tips of pineapple cv 'Queen' [Ananas comosus (L.) Merr.] were initiated using Murashiege and Skoog's basal meduim supplemented with 10mg/liter 4-amino-3,5,6-trichloro-2-pyrodinecarboxylic acid (Picloram). High percentage of growth and shoot proliferation was observed in basal medium supplemented with 50 μM benzene adenine purine (BAP) and 10 μM gibberrelic acid (GA) after 8 weeks in vitro. Regenerants derived from shoots using different doses of gamma rays (0, 5, 10, 15, 20, 25 and 30 Gy) were evaluated under laboratory conditions. Of the different doses, 15 Gy produced the most variegation in young shoots (chlorophyll variants) maintained in vitro. The variants appeared to have yellow and green color combinations of the young leaves of pineapple 'Queen' variety. When transplanted inside the greenhouse, high percentage of plantlets survival was observed, ranging from 90-95%. Of the different irradiation doses, variegation in young leaves was observed at 15, 20, 25 and 30 Gy. Further assessment on the effect of irradiation is currently being unertaken under screen house conditions. The variants produced could serve a basis for selection of ornamental-type pineapple. In addition, protocols developed on the use of in vitro culture techniques could be utilized as a tool for induced mutation breeding in pineapple. (author)

  10. The Mechanical Properties and Microstructure Characters of Hybrid Composite Geopolymers-Pineapple Fiber Leaves (PFL)

    Science.gov (United States)

    Amalia, N.; Hidayatullah, S.; Nurfadilla; Subaer

    2017-03-01

    The objective of this research is to study the influence of organic fibers on the mechanical properties and microstructure characters of hybrid composite geopolymers-pineapple fibers (PFL). Geopolymers were synthesized by using alkali activated of class C-fly ash added manually with short pineapple fiber leaves (PFL) and then cured at 60°C for 1 hour. The resulting composites were stored in open air for 28 days prior to mechanical and microstructure characterizations. The samples were subjected to compressive and flexural strength measurements, heat resistance as well as acid attack (1M H2SO4 solution). The microstructure of the composites were examined by using Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM-EDS). The measurement showed that the addition of pineapple fibers was able to improve the compressive and flexural strength of geopolymers. The resulting hybrid composites were able to resist fire to a maximum temperature of 1500°C. SEM examination showed the presence of good bond between geopolymer matrix and pineapple fibers. It was also found that there were no chemical constituents of geopolymers leached out during acid liquid treatment. It is concluded that hybrid composite geopolymers-pineapple fibers are potential composites for wide range applications.

  11. Genome-wide identification, functional and evolutionary analysis of terpene synthases in pineapple.

    Science.gov (United States)

    Chen, Xiaoe; Yang, Wei; Zhang, Liqin; Wu, Xianmiao; Cheng, Tian; Li, Guanglin

    2017-10-01

    Terpene synthases (TPSs) are vital for the biosynthesis of active terpenoids, which have important physiological, ecological and medicinal value. Although terpenoids have been reported in pineapple (Ananas comosus), genome-wide investigations of the TPS genes responsible for pineapple terpenoid synthesis are still lacking. By integrating pineapple genome and proteome data, twenty-one putative terpene synthase genes were found in pineapple and divided into five subfamilies. Tandem duplication is the cause of TPS gene family duplication. Furthermore, functional differentiation between each TPS subfamily may have occurred for several reasons. Sixty-two key amino acid sites were identified as being type-II functionally divergence between TPS-a and TPS-c subfamily. Finally, coevolution analysis indicated that multiple amino acid residues are involved in coevolutionary processes. In addition, the enzyme activity of two TPSs were tested. This genome-wide identification, functional and evolutionary analysis of pineapple TPS genes provide a new insight into understanding the roles of TPS family and lay the basis for further characterizing the function and evolution of TPS gene family. Copyright © 2017 Elsevier Ltd. All rights reserved.

  12. Study of Tensile Properties and Deflection Temperature of Polypropylene/Subang Pineapple Leaf Fiber Composites

    Science.gov (United States)

    Hafizhah, R.; Juwono, A. L.; Roseno, S.

    2017-05-01

    The development of eco-friendly composites has been increasing in the past four decades because the requirement of eco-friendly materials has been increasing. Indonesia has a lot of natural fiber resources and, pineapple leaf fiber is one of those fibers. This study aimed to determine the influence of weight fraction of pineapple leaf fibers, that were grown at Subang, to the tensile properties and the deflection temperature of polypropylene/Subang pineapple leaf fiber composites. Pineapple leaf fibers were pretreated by alkalization, while polypropylene pellets, as the matrix, were extruded into sheets. Hot press method was used to fabricate the composites. The results of the tensile test and Heat Deflection Temperature (HDT) test showed that the composites that contained of 30 wt.% pineapple leaf fiber was the best composite. The values of tensile strength, modulus of elasticity and deflection temperature were (64.04 ± 3.91) MPa; (3.98 ± 0.55) GPa and (156.05 ± 1.77) °C respectively, in which increased 187.36%, 198.60%, 264.72% respectively from the pristine polypropylene. The results of the observation on the fracture surfaces showed that the failure modes were fiber breakage and matrix failure.

  13. Development and evaluation of a new method for sampling and monitoring the symphylid population in pineapple.

    Science.gov (United States)

    Soler, Alain; Gaude, Jean-Marie; Marie-Alphonsine, Paul-Alex; Vinatier, Fabrice; Dole, Bernard; Govindin, Jean-Claude; Fournier, Patrick; Queneherve, Patrick

    2011-09-01

    Symphylids (Hanseniella sp.) are polyphagous soilborne parasites. Today, symphylid populations on pineapple are monitored by observing root symptoms and the presence of symphylids at the bottom of basal leaves. The authors developed a reliable method with a bait and trap device to monitor symphylid populations in pineapple or fallow crops. The spatial distribution of the symphylid populations was evaluated using the variance/mean ratios and spatial analyses based on Moran's and Geary's indices. The method has been tested to monitor symphylid populations at different developmental stages of pineapple. Adding potato baits to the soil samples increased the trapping efficiency of symphylids when compared with 'soil only' and 'bait only' methods. The handling of the samples is also facilitated by the new device. Results showed that the vertical distribution of symphylids may be uniform deeply inside the soil profile under pineapple, up to 50 cm. Results showed that symphylid populations are highly aggregated, showing a spot area about 4-6 m wide for their development. The new method allows better and easier evaluation of symphylid populations. It may be very useful in the evaluation of new IPM methods to control symphylids under pineapple. Copyright © 2011 Society of Chemical Industry.

  14. Bromelain, a cysteine protease from pineapple (Ananas comosus) stem, is an inhibitor of fungal plant pathogens.

    Science.gov (United States)

    López-García, B; Hernández, M; Segundo, B S

    2012-07-01

    This study aimed to evaluate the effect of bromelain, a cysteine protease isolated from pineapple (Ananas comosus), on growth of several agronomically important fungal pathogens. Purification of bromelain from pineapple stems was carried out by chromatography techniques, and its antimicrobial activity was tested against the fungal pathogens Fusarium verticillioides, Fusarium oxysporum and Fusarium proliferatum by broth microdilution assay. A concentration of 0.3 μmol l(-1) of bromelain was sufficient for 90% growth inhibition of F. verticillioides. The capability of bromelain to inhibit fungal growth is related to its proteolytic activity. The study demonstrates that stem bromelain exhibits a potent antifungal activity against phytopathogens and suggests its potential use as an effective agent for crop protection. The results support the use of a natural protease that accumulates at high levels in pineapple stems as alternative to the use of chemical fungicides for crop protection. © 2012 The Authors. Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.

  15. Applications of Microencapsulated Bifidobacterium Longum with Eleutherine Americana in Fresh Milk Tofu and Pineapple Juice

    Science.gov (United States)

    Phoem, Atchara N.; Chanthachum, Suphitchaya; Voravuthikunchai, Supayang P.

    2015-01-01

    Bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. Microencapsulation of B. longum with Eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. Microencapsulated B. longum with the extract and oligosaccharides extract in the food products showed better survival than free cells under adverse conditions. Sensory analysis demonstrated that the products containing co-encapsulated bacterial cells were more acceptable by consumers than free cells. Pineapple juice prepared with co-encapsulated cells had lower values for over acidification, compared with the juice with free cells added. This work suggested that microencapsulated B. longum with E. americana could enhance functional properties of fresh milk tofu and pineapple juice. PMID:25854832

  16. Postharvest Exogenous Application of Abscisic Acid Reduces Internal Browning in Pineapple.

    Science.gov (United States)

    Zhang, Qin; Liu, Yulong; He, Congcong; Zhu, Shijiang

    2015-06-10

    Internal browning (IB) is a postharvest physiological disorder causing economic losses in pineapple, but there is no effective control measure. In this study, postharvest application of 380 μM abscisic acid (ABA) reduced IB incidence by 23.4-86.3% and maintained quality in pineapple fruit. ABA reduced phenolic contents and polyphenol oxidase and phenylalanine ammonia lyase activities; increased catalase and peroxidase activities; and decreased O2(·-), H2O2, and malondialdehyde levels. This suggests ABA could control IB through inhibiting phenolics biosynthesis and oxidation and enhancing antioxidant capability. Furthermore, the efficacy of IB control by ABA was not obviously affected by tungstate, ABA biosynthesis inhibitor, nor by diphenylene iodonium, NADPH oxidase inhibitor, nor by lanthanum chloride, calcium channel blocker, suggesting that ABA is sufficient for controlling IB. This process might not involve H2O2 generation, but could involve the Ca(2+) channels activation. These results provide potential for developing effective measures for controlling IB in pineapple.

  17. Physicochemical changes of 'Phulae' pineapple fruit treated with short-term anoxia during ambient storage.

    Science.gov (United States)

    Techavuthiporn, Chairat; Boonyaritthongchai, Panida; Supabvanich, Suriyan

    2017-08-01

    The effects of short-term anoxia exposure for 16h on physicochemical changes of 'Phulae' pineapple fruit stored at ambient temperature (25±2°C) were investigated. The respiratory rate of the fruit was induced by the anoxia treatment. However, it retarded the increase in moisture loss and maintained both flesh and pulp colour by inhibiting polyphenol oxidase (PPO) activity of the both tissues. The anoxia exposure delayed the increase in total sugar content and enhanced total ascorbic acid content during storage. The half-cut pineapple fruit showed that the anoxia exposure completely inhibited internal transparency of the flesh tissue adjacent to core during the storage. In conclusion, the short-term anoxia exposure for 16h maintained postharvest quality, retarded physiological disorder and enhanced nutritional values of the pineapple fruit stored at ambient temperature (25±2°C). Copyright © 2017 Elsevier Ltd. All rights reserved.

  18. Applications of Microencapsulated Bifidobacterium Longum with Eleutherine Americana in Fresh Milk Tofu and Pineapple Juice

    Directory of Open Access Journals (Sweden)

    Atchara N. Phoem

    2015-04-01

    Full Text Available Bifidobacterium longum was microencapsulated by extrusion technique and added in fresh milk tofu and pineapple juice. Microencapsulation of B. longum with Eleutherine americana extract, oligosaccharides extract, and commercial fructo-oligosaccharides was assessed for the bacterial survival after sequential exposure to simulated gastric and intestinal juices, and refrigeration storage. Microencapsulated B. longum with the extract and oligosaccharides extract in the food products showed better survival than free cells under adverse conditions. Sensory analysis demonstrated that the products containing co-encapsulated bacterial cells were more acceptable by consumers than free cells. Pineapple juice prepared with co-encapsulated cells had lower values for over acidification, compared with the juice with free cells added. This work suggested that microencapsulated B. longum with E. americana could enhance functional properties of fresh milk tofu and pineapple juice.

  19. SOLID AND LIQUID PINEAPPLE WASTE UTILIZATION FOR LACTIC ACID FERMENTATION USING Lactobacillus delbrueckii

    Directory of Open Access Journals (Sweden)

    Abdullah Abdullah

    2012-01-01

    Full Text Available The liquid and solid  pineapple wastes contain mainly sucrose, glucose, fructose and other nutrients. It therefore can potentially be used as carbon source for fermentation to produce organic acid. Recently, lactic acid has been considered to be an important raw material for production of biodegradable lactate polymer. The experiments were  carried out in batch fermentation using  the  liquid and solid pineapple wastes to produce lactic acid. The anaerobic fermentation of lactic acid were performed at 40 oC, pH 6, 5% inocolum and  50 rpm. Initially  results show that the liquid pineapple waste by  using Lactobacillus delbrueckii can be used as carbon source  for lactic acid fermentation. The production of lactic acid  are found to be 79 % yield, while only  56% yield was produced by using solid waste. 

  20. Irradiation on cereal bars incorporated with pineapple skin

    International Nuclear Information System (INIS)

    Souza, Adriana Regia Marques; Silva, Yasmini Portes Abraham; Costa, Naiane Vieira; Almeida, Thatyana Lacerda; Damiani, Clarissa; Arthur, Valter; Lage, Moacir Evandro; Asquieri, Eduardo Ramirez

    2011-01-01

    One of the major current concerns to the food industry is the management of residues generated in its production processes. Thus, several studies have been developed, seeking alternative uses for these residues, in order to minimize environmental impacts and add value to products previously discarded. Combining this idea with the increasingly search for healthy and practical products, by consumers, this study aimed the characterization of cereal bars irradiated with doses of 0 kGy, 1 kGy, and 2 kGy, incorporated with dried pineapple skin. The following analyses were carried out: moisture, proteins, lipids, ashes, carbohydrates, energetic value, antioxidant potential, phenolic content, organic acids, and fatty acids profile. The results observed for the centesimal composition did not vary as a function of the radiation doses used, reducing only the levels of antioxidants, phenolic compounds, and organic acids. The product showed potential for becoming an effective way of reusing a food industry residue and the irradiation interfered on the nutritional characteristics of the final product. (author)

  1. Developmental toxicity of orally administered pineapple leaf extract in rats.

    Science.gov (United States)

    Hu, Jun; Lin, Han; Shen, Jia; Lan, Jiaqi; Ma, Chao; Zhao, Yunan; Lei, Fan; Xing, Dongming; Du, Lijun

    2011-06-01

    The extract of pineapple leaves (EPL) has anti-diabetic and anti-dyslipidemic effects and can be developed into a promising natural medicine. This study was conducted to evaluate EPL's effects on developmental parameters in order to provide evidence of its safety before potential medical use. Five groups were included: a negative control that was given distilled water daily, a positive control that was dosed 7 mg/kg cyclophosphamide (CP) every two days, and three groups that were respectively dosed 2.0, 1.0, and 0.5 g/kg EPL daily. Female rats were dosed during the organogenesis period of gestation days (GD) 7-17 and terminated on GD 20. A series of parameters were examined. Data revealed that CP significantly reduced maternal body weight gains, caused maternal organ weight alterations, reduced female fertility, disturbed fetal growth and development, and caused marked teratogenic effects on fetal appearances, skeleton and internal organs. Distilled water and the three high doses of EPL did not cause any of the aforementioned effects. This study concluded that orally administered EPL is safe to rats during embryonic development. Copyright © 2011 Elsevier Ltd. All rights reserved.

  2. A Review on Pineapple Leaves Fibre and Its Composites

    Directory of Open Access Journals (Sweden)

    M. Asim

    2015-01-01

    Full Text Available Natural fibre based composites are under intensive study due to their ecofriendly nature and peculiar properties. The advantage of natural fibres is their continuous supply, easy and safe handling, and biodegradable nature. Although natural fibres exhibit admirable physical and mechanical properties, it varies with the plant source, species, geography, and so forth. Pineapple leave fibre (PALF is one of the abundantly available wastes materials of Malaysia and has not been studied yet as it is required. A detailed study of chemical, physical, and mechanical properties will bring out logical and reasonable utilization of PALF for various applications. From the socioeconomic prospective, PALF can be a new source of raw material to the industries and can be potential replacement of the expensive and nonrenewable synthetic fibre. However, few studies on PALF have been done describing the interfacial adhesion between fibres and reinforcement compatibility of fibre but a detailed study on PALF properties is not available. In this review, author covered the basic information of PALF and compared the chemical, physical, and mechanical properties with other natural fibres. Furthermore, it summarizes the recent work reported on physical, mechanical, and thermal properties of PALF reinforced polymer composites with its potential applications.

  3. Macronutrient uptake, accumulation and export by the irrigated 'vitória' pineapple plant

    Directory of Open Access Journals (Sweden)

    Rodinei Facco Pegoraro

    2014-06-01

    Full Text Available The nutritional state of the pineapple plant has a large effect on plant growth, on fruit production, and fruit quality. The aim of this study was to assess the uptake, accumulation, and export of nutrients by the irrigated 'Vitória' pineapple plant during and at the end of its development. A randomized block statistical design with four replications was used. The treatments were defined by different times of plant collection: at 270, 330, 390, 450, 510, 570, 690, 750, and 810 days after planting (DAP. The collected plants were separated into the following components: leaves, stem, roots, fruit, and slips for determination of fresh and dry matter weight at 65 ºC. After drying, the plant components were ground for characterization of the composition and content of nutrients taken up and exported by the pineapple plant. The results were subjected to analysis of variance, and non-linear regression models were fitted for the significant differences identified by the F test (p N > S > Ca > Mg > P, which corresponded to 898, 452, 134, 129, 126, and 107 kg ha-1, respectively, of total accumulation. The export of macronutrients by the pineapple fruit was in the following decreasing order: K > N > S > Ca > P > Mg, which was equivalent to 18, 17, 11, 8, 8, and 5 %, respectively, of the total accumulated by the pineapple. The 'Vitória' pineapple plant exported 78 kg ha-1 of N, 8 kg ha-1 of P, 164 kg ha-1 of K, 14 kg ha-1 of S, 10 kg ha-1 of Ca, and 6 kg ha-1 of Mg by the fruit. The nutrient content exported by the fruits represent important components of nutrient extraction from the soil, which need to be restored, while the nutrients contained in the leaves, stems and roots can be incorporated in the soil within a program of recycling of crop residues.

  4. Genetic Diversity in Various Accessions of Pineapple [Ananas comosus (L.) Merr.] Using ISSR and SSR Markers.

    Science.gov (United States)

    Wang, Jian-Sheng; He, Jun-Hu; Chen, Hua-Rui; Chen, Ye-Yuan; Qiao, Fei

    2017-12-01

    Inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers were used to assess the genetic diversity of 36 pineapple accessions that were introduced from 10 countries/regions. Thirteen ISSR primers amplified 96 bands, of which 91 (93.65%) were polymorphic, whereas 20 SSR primers amplified 73 bands, of which 70 (96.50%) were polymorphic. Nei's gene diversity (h = 0.28), Shannon's information index (I = 0.43), and polymorphism information content (PIC = 0.29) generated using the SSR primers were higher than that with ISSR primers (h =  0.23, I = 0.37, PIC = 0.24), thereby suggesting that the SSR system is more efficient than the ISSR system in assessing genetic diversity in various pineapple accessions. Mean genetic similarities were 0.74, 0.61, and 0.69, as determined using ISSR, SSR, and combined ISSR/SSR, respectively. These results suggest that the genetic diversity among pineapple accessions is very high. We clustered the 36 pineapple accessions into three or five groups on the basis of the phylogenetic trees constructed based on the results of ISSR, SSR, and combined ISSR/SSR analyses using the unweighted pair-group with arithmetic averaging (UPGMA) method. The results of principal components analysis (PCA) also supported the UPGMA clustering. These results will be useful not only for the scientific conservation and management of pineapple germplasm but also for the improvement of the current pineapple breeding strategies.

  5. Diurnal Cycling Transcription Factors of Pineapple Revealed by Genome-Wide Annotation and Global Transcriptomic Analysis.

    Science.gov (United States)

    Sharma, Anupma; Wai, Ching Man; Ming, Ray; Yu, Qingyi

    2017-09-01

    Circadian clock provides fitness advantage by coordinating internal metabolic and physiological processes to external cyclic environments. Core clock components exhibit daily rhythmic changes in gene expression, and the majority of them are transcription factors (TFs) and transcription coregulators (TCs). We annotated 1,398 TFs from 67 TF families and 80 TCs from 20 TC families in pineapple, and analyzed their tissue-specific and diurnal expression patterns. Approximately 42% of TFs and 45% of TCs displayed diel rhythmic expression, including 177 TF/TCs cycling only in the nonphotosynthetic leaf tissue, 247 cycling only in the photosynthetic leaf tissue, and 201 cycling in both. We identified 68 TF/TCs whose cycling expression was tightly coupled between the photosynthetic and nonphotosynthetic leaf tissues. These TF/TCs likely coordinate key biological processes in pineapple as we demonstrated that this group is enriched in homologous genes that form the core circadian clock in Arabidopsis and includes a STOP1 homolog. Two lines of evidence support the important role of the STOP1 homolog in regulating CAM photosynthesis in pineapple. First, STOP1 responds to acidic pH and regulates a malate channel in multiple plant species. Second, the cycling expression pattern of the pineapple STOP1 and the diurnal pattern of malate accumulation in pineapple leaf are correlated. We further examined duplicate-gene retention and loss in major known circadian genes and refined their evolutionary relationships between pineapple and other plants. Significant variations in duplicate-gene retention and loss were observed for most clock genes in both monocots and dicots. © The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

  6. EVALUATION OF ANTHELMINTIC ACTIVITY OF PINEAPPLE FRUIT EXTRACT USING INDIAN EARTHWORM (PHERITIMA POSTHUMA)

    OpenAIRE

    Dey P; Debnath P; Bhakta T

    2013-01-01

    Pineapple is a favorite for the lovers of fruit in its fresh forms as well as in preserves like jams, jellies and squashes. Two varieties of Pineapples (Queen and Kew) are available in Tripura during mid-May to mid-September. The plant is well known for its different folk medicines like the root and fruit are either eaten or applied topically as an anti-inflammatory, digestive and anthelmintic. It was observed that the people of Tripura especially the Tribes are use the juice of matured root ...

  7. Microwaves as a pretreatment for enhancing enzymatic hydrolysis of pineapple industrial waste for bioethanol production

    OpenAIRE

    Conesa Domínguez, Claudia; Seguí Gil, Lucía; Laguarda-Miro, Nicolas; Fito Maupoey, Pedro

    2016-01-01

    [EN] The pineapple industry generates significant amounts of residues which are classified as lignocellulosic residual biomass. In the present paper, microwaves are studied as a pretreatment to improve pineapple waste saccharification. Different microwave (MW) powers (10.625, 8.5, 6.375, 4.25 and 2.125 W/g) and exposure times (1-20 min) were applied to the solid part of the waste before enzymatic hydrolysis. Infrared thermography was used to assess temperature evolution and structural modific...

  8. Optimization of a protocol for the micropropagation of pineapple

    Directory of Open Access Journals (Sweden)

    ALMEIDA WELITON ANTONIO BASTOS DE

    2002-01-01

    Full Text Available The present work aimed at maximizing the number of plantlets obtained by the micropropagation of pineapple (Ananas comosus (L. Merrill cv. Pérola. Changes in benzylaminopurine (BAP concentration, type of medium (liquid or solidified and the type of explant in the proliferation phase were evaluated. Slips were used as the explant source, which consisted of axillary buds obtained after careful excision of the leaves. A Sterilization was done in the hood with ethanol (70%, for three minutes, followed by calcium hypochlorite (2%, for fifteen minutes, and three washes in sterile water. The explants were introduced in MS medium supplemented with 2mg L-1 BAP and maintained in a growth room at a 16h photoperiod (40 mmol.m-2.s-1, 27 ± 2ºC. After eight weeks, cultures were subcultured for multiplication in MS medium. The following treatments were tested: liquid x solidified medium with different BAP concentrations (0.0, 1.5 or 3.0 mg L-1, and the longitudinal cut, or not, of the shoot bud used as explant. The results showed that liquid medium supplemented with BAP at 1.5 mg L-1, associated with the longitudinal sectioning of the shoot bud used as explant presented the best results, maximizing shoot proliferation. On average, the best treatment would allow for an estimated production of 161,080 plantlets by the micropropagation of the axillary buds of one plant with eight slips and ten buds/slips, within a period of eight months.

  9. Effect of graded levels of dry pineapple peel on digestibility and ...

    African Journals Online (AJOL)

    SARAH

    2013-07-30

    Jul 30, 2013 ... ABSTRACT. Objectives: A feeding trial was conducted to investigate the effect of the inclusion levels of sun dried pineapple peel (PP) in the diets, on the digestibility and growth performance of rabbits. Methodology and Results: Diets R0 (control), R20, R30 and R40 were formulated by including 0, 20, 30.

  10. Utilization of Wheat Offal-Carried Pineapple Waste in the Diet of ...

    African Journals Online (AJOL)

    Utilization of Wheat Offal-Carried Pineapple Waste in the Diet of West African Dwarf (WAD) Goats. ... Although, the analysis of blood cells (red blood cell, white blood and packed cell volume counts) were significantly different (p<0.05) among the goats fed experimental diets, the counts fell within the normal physiological ...

  11. Physical and chemical pretreatment of lignocellulosics in pineapple (Ananus comosus) peels dried for investment

    Science.gov (United States)

    Sukkaew, Adulsman; Boonsong, Panthip; Thongpradistha, Sriubol; Intan, Maimoon

    2017-08-01

    Pineapple (Ananus comosus) Peels, once known as waste from agricultural, can be a problem when we eliminate in agriculture and industry. The current technology can help preliminarily to solve this problem. The sustainable solution to this problem is lignocellulosics pretreatments for converted saccharide as a carbon source for ethanol production. The objective of this study is the investigation of pineapple peels pretreatment to produce fermentable sugar by drying and digesting 5% sulfuric acid (H2SO4). And study of cost economic passed selection for investment. The result found that the best investment of drying was 100 °C at 11 hours for the sulphuric acid which could be easily crushed into a fine powder. Moreover, digestion of pineapple peels gave the best total sugar 252.2 g/l by 5% H2SO4 incubated for 60 minutes at room temperature. The pineapple peels were digested by 5%H2SO4 concentration by incubating for 60 minutes at room temperature, finding to be the best condition and the lowest investment. Finally, the optimisation of investment and management for lignocellulosic pretreatment will improve efficiency of strategy for economic and energy development.

  12. Utilization of Cellulose from Pineapple Leaf Fibers as Nanofiller in Polyvinyl Alcohol-Based Film

    Directory of Open Access Journals (Sweden)

    Kendri Wahyuningsih

    2016-08-01

    Full Text Available Cellulose from pineapple leaf fibers as one of the natural polymer which has biodegradable property in a nanometer’s scale, can be formed as a filler in composite of Poly(vinyl Alcohol/PVA is expected to increase the physical, thermal, and barrier properties of composite films similar to conventional plastic. The aim of this study was to examine the effect of fibrillation of cellulose fibers from pineapple leaf fibers using a combined technique of chemical-mechanical treatments, to investigate the reinforcing effect of concentration of nanocellulose fibrils in the polyvinyl alcohol (PVA matrix on physical properties, thermal properties, water vapor transmission rate, light transmittance and morphological with and without addition of glycerol. Nanocellulose was made from cellulose of pineapple leaf fiber using wet milling (Ultra Fine Grinder. The composite film production was carried out by using casting solution method by mixing PVA solution with nanocellulose (10-50% and glycerol (0-1%. The characterization of film covered physical properties (thickness, moisture content and density, thermal properties, permeability (WVTR, light transmittance, morphology, and crystallinity. Nanocellulose from pineapple leaf fibers was produced by Ultra Fine Grinder shows that the size reduction process was accurate. Nanocellulose addition on PVA composite film was affected to increasing the physical, thermal, and barrier properties. Meanwhile, decreasing the percentage of composite film transmittance, thus the transparency decrease (opaque. Water vapor transmission rate (WVTR the film was increased with increasing glycerol concentration, but the physical and thermal properties was decreased.

  13. Inter-fertility among female parent clones of pineapple involved in a ...

    African Journals Online (AJOL)

    Inter-fertility among female parent clones of pineapple involved in a 6x6 complete diallel crossing system based on typological approach. AE Issali, AE Badou Nguessan, DF Thiemele, A Amoncho, SB Ngoran Koua, HC Keassemon Kone, ZEB Koffi, A Sangare ...

  14. Effect of gas sparging on flux enhancement and phytochemical properties of clarified pineapple juice by microfiltration

    KAUST Repository

    Laorko, Aporn; Li, Zhenyu; Tongchitpakdee, Sasitorn; Youravong, Wirote

    2011-01-01

    of the membrane process. In this study, a 0.2 μm hollow fiber microfiltration membrane was used to study the effect of cross flow velocity (CFV) and gas injection factor () on the critical and limiting flux during microfiltration of pineapple juice. In addition

  15. Species Richness, Community Organization, and Spatiotemporal Distribution of Earthworms in the Pineapple Agroecosystems of Tripura, India

    Directory of Open Access Journals (Sweden)

    Animesh Dey

    2016-01-01

    Full Text Available The impact that plant communities may have on underground faunal diversity is unclear. Therefore, understanding the links between plants and organisms is of major interest. Earthworm population dynamics were studied in the pineapple agroecosystems of Tripura to evaluate the impact of monoculture plantation on earthworm communities. A total of thirteen earthworm species belonging to four families and five genera were collected from different sampling sites. Application of sample-based rarefaction curve and nonparametric richness estimators reveal 90–95% completeness of sampling. Earthworm community of pineapple agroecosystems was dominated by endogeic earthworms and Drawida assamensis was the dominant species with respect to its density, biomass, and relative abundance. Vertical distribution of earthworms was greatly influenced by seasonal variations. Population density and biomass of earthworms peaked during monsoon and postmonsoon period, respectively. Overall density and biomass of earthworms were in increasing trend with an increase in plantation age and were highest in the 30–35-year-old plantation. Significant decrease in the Shannon diversity and evenness index and increase in Simpson’s dominance and spatial aggregation index with an increase in the age of pineapple plantation were recorded. Soil temperature and soil moisture were identified as the most potent regulators of earthworm distribution in the pineapple plantation.

  16. Agrobacterium-mediated genetic transformation of pineapple (Ananas comosus L., Merr.).

    Science.gov (United States)

    Mhatre, Minal

    2013-01-01

    Pineapple (Ananas comosus L., Merr.) is a commercially important crop, grown in the tropical and subtropical regions. However, the crop is faced with postharvest damage and poor varietal and nutritional improvement. Being a vegetatively propagated crop, conventional breeding programs take longer time for genetic improvement, which may not necessarily successfully develop an improved cultivar. Hence, the genetic modification of pineapple is an alternative handy approach to improve pineapple. We have established an Agrobacterium-mediated transformation system using leaf bases from in vitro-grown pineapple plants. Being a monocot, acetosyringone is added to the culture medium for overnight growth of Agrobacterium and transformation to transfer a gene of interest MSI99 soybean ferritin. Leaf bases isolated from in vitro shoot cultures are treated with Agrobacterium suspension at two dilutions, 10× and 20×, for 30 min. Explants are subsequently blot dried and cultured on gelrite solidified hormone-free Pin1 medium for 2 days (cocultivation). Periodic transfer is first done to the regeneration medium (Pin1) containing cefotaxime for the suppression of Agrobacterium growth. The transformants are selected by culturing on Pin1 medium containing cefotaxime and kanamycin. Multiple shoots, regenerated in leaf bases, are further multiplied and individually rooted in the liquid RM medium amended with antibiotics to recover plants. Putative transformants are analyzed for transgene integration and expression using standard molecular biological methods of PCR, RT-PCR, and genomic Southern.

  17. Simulation and Failure Analysis of Car Bumper Made of Pineapple Leaf Fiber Reinforced Composite

    Science.gov (United States)

    Arbintarso, E. S.; Muslim, M.; Rusianto, T.

    2018-02-01

    The bumper car made of the Pineapple Leaf Fiber Reinforced Composite (PLFRC) is possible to be produced with the advantage of easy to get, and cheap. Pineapple leaf fiber has chosen as a natural fiber, which the maximum of the strength of 368 MPa. The objective of this study was to determine the maximum capability of front car bumpers using Pineapple Leaf Fiber Reinforced Composite materials through the process of simulating stress analysis with Solidworks 2014 software. The aim also to know the distribution of loads that occur on the front car bumper and predict the critical point position on the design of the bumper. The result will use to develop the alternative lightweight, cheap and environmentally friendly materials in general and the development of the use of pineapple fiber for automotive purposes in particular. Simulations and failure analysis have been conducted and showed an increased impact speed in line with increased displacement, strain, and stress that occur on the surface of the bumper. The bumper can withstand collisions at a speed of less than 70 kph.

  18. Structural characteristics of pineapple pulp polysaccharides and their antitumor cell proliferation activities.

    Science.gov (United States)

    Wang, Ling; Tang, De-Qiang; Kuang, Yu; Lin, Feng-Jiao; Su, Yu

    2015-09-01

    Pineapple has a delicious taste and good health benefits. Bioactive polysaccharides are important components of pineapple that might contribute to its health benefits. Since little structural information on these polysaccharides is currently available, the aim of this study was to investigate their structural characteristics and bioactivities. The polysaccharides of pineapple pulp were fractionated into three fractions (PAPs 1-3) by anion exchange chromatography. Their structural characteristics were first identified, including molecular weights and glycosidic linkages. The monosaccharide compositions were revealed as PAP 1 (Ara, Xyl, Man, Glc and Gal), PAP 2 (Rha, Ara, Xyl, Man, Glc and Gal) and PAP 3 (Rha, Ara, Xyl, Man and Gal). Nuclear magnetic resonance (NMR) spectra suggested that PAP 2 had a backbone of → 4)-α-d-Manp-(1 → 2,4)-α-d-Manp-(1 → with branches attached to O-4 of Manp. The NMR data of α-l-Araf-(1→, →3)-α-l-Araf-(1→, →4)-β-d-Galp-(1 → and → 4)-α-d-GalpAMe-(1 → were assigned. PAPs 1 and 2 showed significant antitumor cell proliferation activities against breast carcinoma cell line and strong antioxidant activities. The above findings indicated that PAPs 1-3 contributed much to the health benefits of pineapple. They could be used as health-beneficial food additives in functional foods. © 2015 Society of Chemical Industry.

  19. Quality challenges and opportunities in the pineapple supply chain in Benin

    NARCIS (Netherlands)

    Arinloye, D.D.A.A.; Boekel, van M.A.J.S.

    2016-01-01

    Quality has become a key aspect for establishing international market access and improving competitiveness of (smallholder) producers in developing countries. This is especially the case for perishable tropical food products. This chapter explores the key quality issues of the Beninese pineapple

  20. Droplet vitrification technique for cryopreservation of different pineapple (Ananas comosus L. Merrill) accessions

    Science.gov (United States)

    Germplasm conservation of pineapple is crucial to secure the genetic variability of the genus for breeding programs and supporting new research. Long-term conservation is done through cryopreservation, by storing cells or tissues at ultra-low temperature in liquid nitrogen (LN; -196°C) or in the LN ...

  1. Physico-chemical properties, antioxidant activity and mineral contents of pineapple genotypes grown in china.

    Science.gov (United States)

    Lu, Xin-Hua; Sun, De-Quan; Wu, Qing-Song; Liu, Sheng-Hui; Sun, Guang-Ming

    2014-06-23

    The fruit physico-chemical properties, antioxidant activity and mineral contents of 26 pineapple [Ananas comosus (L.) Merr.] genotypes grown in China were measured. The results showed great quantitative differences in the composition of these pineapple genotypes. Sucrose was the dominant sugar in all 26 genotypes, while citric acid was the principal organic acid. Potassium, calcium and magnesium were the major mineral constituents. The ascorbic acid (AsA) content ranged from 5.08 to 33.57 mg/100 g fresh weight (FW), while the total phenolic (TP) content varied from 31.48 to 77.55 mg gallic acid equivalents (GAE)/100 g FW. The two parameters in the predominant cultivars Comte de Paris and Smooth Cayenne were relative low. However, MD-2 indicated the highest AsA and TP contents (33.57 mg/100 g and 77.55 mg GAE/100 g FM, respectively), and it also showed the strongest antioxidant capacity 22.85 and 17.30 μmol TE/g FW using DPPH and TEAC methods, respectively. The antioxidant capacity of pineapple was correlated with the contents of phenolics, flavonoids and AsA. The present study provided important information for the further application of those pineapple genotypes.

  2. Transcriptome Profiling of the Pineapple under Low Temperature to Facilitate Its Breeding for Cold Tolerance

    Science.gov (United States)

    Chen, Chengjie; Zhang, Yafeng; Xu, Zhiqiang; Luan, Aiping; Mao, Qi; Feng, Junting; Xie, Tao; Gong, Xue; Wang, Xiaoshuang; Chen, Hao; He, Yehua

    2016-01-01

    The pineapple (Ananas comosus) is cold sensitive. Most cultivars are injured during winter periods, especially in sub-tropical regions. There is a lack of molecular information on the pineapple’s response to cold stress. In this study, high-throughput transcriptome sequencing and gene expression analysis were performed on plantlets of a cold-tolerant genotype of the pineapple cultivar ‘Shenwan’ before and after cold treatment. A total of 1,186 candidate cold responsive genes were identified, and their credibility was confirmed by RT-qPCR. Gene set functional enrichment analysis indicated that genes related to cell wall properties, stomatal closure and ABA and ROS signal transduction play important roles in pineapple cold tolerance. In addition, a protein association network of CORs (cold responsive genes) was predicted, which could serve as an entry point to dissect the complex cold response network. Our study found a series of candidate genes and their association network, which will be helpful to cold stress response studies and pineapple breeding for cold tolerance. PMID:27656892

  3. DNA profiling of pineapple cultivars in Japan discriminated by SSR markers

    Science.gov (United States)

    Shoda, Moriyuki; Urasaki, Naoya; Sakiyama, Sumisu; Terakami, Shingo; Hosaka, Fumiko; Shigeta, Narumi; Nishitani, Chikako; Yamamoto, Toshiya

    2012-01-01

    We developed 18 polymorphic simple sequence repeat (SSR) markers in pineapple (Ananas comosus) by using genomic libraries enriched for GA and CA motifs. The markers were used to genotype 31 pineapple accessions, including seven cultivars and 11 breeding lines from Okinawa Prefecture, 12 foreign accessions and one from a related species. These SSR loci were highly polymorphic: the 31 accessions contained three to seven alleles per locus, with an average of 4.1. The values of expected heterozygosity ranged from 0.09 to 0.76, with an average of 0.52. All 31 accessions could be successfully differentiated by the 18 SSR markers, with the exception of ‘N67-10’ and ‘Hawaiian Smooth Cayenne’. A single combination of three markers TsuAC004, TsuAC010 and TsuAC041, was enough to distinguish all accessions with one exception. A phenogram based on the SSR genotypes did not show any distinct groups, but it suggested that pineapples bred in Japan are genetically diversed. We reconfirmed the parentage of 14 pineapple accessions by comparing the SSR alleles at 17 SSR loci in each accession and its reported parents. The obtained information will contribute substantially to protecting plant breeders’ rights. PMID:23341750

  4. Developing Single Nucleotide Polymorphism (SNP) markers for the identification of pineapple (Ananas comosus) germplasm

    Science.gov (United States)

    Pineapple (Ananas comosus [L.] Merr.) is the third most important tropical fruit in the world after banana and mango and a major agricultural commodity in Hawaii. As a crop with vegetative propagation, genetic redundancy is a major challenge for efficient genebank management and in breeding. Using E...

  5. Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes

    Science.gov (United States)

    Germplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus’ genetic diversity to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation that is done by storing cells or t...

  6. 78 FR 26540 - Importation of Jackfruit, Pineapple, and Starfruit From Malaysia Into the Continental United States

    Science.gov (United States)

    2013-05-07

    ... insect pests, inspected, and imported in commercial consignments. There would also be additional.... viridis, green scale. Darna trima, a nettle caterpillar. D. neobrevipes Beardsley, gray pineapple mealybug... phytopathogenic fungus. Melanitis leda, evening brown butterfly. Parasa lepida, blue-striped nettle grub. P. minor...

  7. Characterization and extraction of volatile compounds from pineapple (Ananas comosus L. Merril processing residues

    Directory of Open Access Journals (Sweden)

    Lília Calheiros de Oliveira Barretto

    2013-12-01

    Full Text Available The aim of this study was to extract and identify volatile compounds from pineapple residues generated during concentrated juice processing. Distillates of pineapple residues were obtained using the following techniques: simple hydrodistillation and hydrodistillation by passing nitrogen gas. The volatile compounds present in the distillates were captured by the solid-phase microextraction technique. The volatile compounds were identified in a system of high resolution gas chromatography system coupled with mass spectrometry using a polyethylene glycol polar capillary column as stationary phase. The pineapple residues constituted mostly of esters (35%, followed by ketones (26%, alcohols (18%, aldehydes (9%, acids (3% and other compounds (9%. Odor-active volatile compounds were mainly identified in the distillate obtained using hydrodistillation by passing nitrogen gas, namely decanal, ethyl octanoate, acetic acid, 1-hexanol, and ketones such as γ-hexalactone, γ-octalactone, δ-octalactone, γ-decalactone, and γ-dodecalactone. This suggests that the use of an inert gas and lower temperatures helped maintain higher amounts of flavor compounds. These data indicate that pineapple processing residue contained important volatile compounds which can be extracted and used as aroma enhancing products and have high potential for the production of value-added natural essences.

  8. Early histological, hormonal, and molecular changes during pineapple (Ananas comosus (L.) Merrill) artificial flowering induction.

    Science.gov (United States)

    Espinosa, Maita Eulalia Ávila; Moreira, Rafael Oliveira; Lima, André Almeida; Ságio, Solange Aparecida; Barreto, Horllys Gomes; Luiz, Sara Lazara Pérez; Abreu, Carlos Eduardo Aragón; Yanes-Paz, Ermis; Ruíz, Yanelis Capdesuñer; González-Olmedo, Justo Lorenzo; Chalfun-Júnior, Antonio

    2017-02-01

    Natural flowering can cause serious scheduling problems in the pineapple (Ananas comosus) industry and increase harvest costs. Pineapple flowering is thought to be triggered by increased ethylene levels and artificial forcing of pineapple flowering is a common practice to promote flowering synchronisation. However, little is known about the early hormonal and molecular changes of pineapple flowering induction and development. Here, we aimed to analyse the molecular, hormonal, and histological changes during artificial pineapple flowering by Ethrel ® 48 treatment. Histological analyses of the shoot apical meristem, leaf gibberellic acid (GA 3 ), and ethylene quantification were carried out during the first 72h after Ethrel ® 48 treatment. Expression profiles from ethylene biosynthesis (AcACS2 and AcACO1), gibberellin metabolism (AcGA2-ox1 and AcDELLA1), and flower development (FT-like gene (AcFT), LFY-like gene (AcLFY), and a PISTILLATA-like gene (AcPI)) genes were analysed during the first 24h after Ethrel ® 48 treatment. Differentiation processes of the shoot apical meristem into flower buds were already present in the first 72h after Ethrel ® 48 treatment. Ethrel ® 48 lead to a reduction in GA 3 levels, probably triggered by elevated ethylene levels and the positive regulation AcGA2-ox1. AcLFY activation upon Ethrel ® 48 may also have contributed to the reduction of GA 3 levels and, along with the up-regulation of AcPI, are probably associated with the flower induction activation. AcFT and AcDELLA1 do not seem to be regulated by GA 3 and ethylene. Decreased GA 3 and increased ethylene levels suggest an accumulation of AcDELLA1, which may display an important role in pineapple flowering induction. Thus, this study shows that molecular, hormonal, and histological changes are present right after Ethrel ® 48 treatment, providing new insights into how pineapple flowering occurs under natural conditions. Copyright © 2016 Elsevier GmbH. All rights reserved.

  9. Enzymatic, antimicrobial and toxicity studies of the aqueous extract of Ananas comosus (pineapple) crown leaf.

    Science.gov (United States)

    Dutta, Sangita; Bhattacharyya, Debasish

    2013-11-25

    Various parts of the plant pineapple (Ananas comosus) are used in traditional medicine worldwide for treatment of a number of diseases and disorders. In folk medicine, pineapple leaf extract was used as an antimicrobial, vermicide, purgative, emmenagoogue, abortifacient, anti-oedema and anti-inflammatory agent. Compared to the fruit and stem extracts of pineapple, information about its leaf extract is limited. The potential of pineapple crown leaf extract as an ethno-medicine has been evaluated in terms of its enzymatic activities related to wound healing, antimicrobial property and toxicity. Major protein components of the extract were revealed by 2-D gel electrophoresis followed by MS/MS analysis. Zymography, DQ-gelatin assay were performed to demonstrate proteolytic, fibrinolytic, gelatinase and collagenase activities. DNase and RNase activities were revealed from agarose gel electrophoresis. Antimicrobial activity was evaluated spectrophotometrically from growth inhibition. Sprague-Dawley rat model was used to measure acute and sub-acute toxicity of the extract by analyzing blood markers. The extract contains several proteins that were clustered under native condition. Proteomic studies indicated presence of fruit bromelain as major protein constituent of the extract. It showed nonspecific protease activity, gelatinolytic, collagenase, fibrinolytic, acid and alkaline phosphatase, peroxidase, DNase and RNase activities along with considerable anti-microbial property. The leaf extract did not induce any toxicity in rats after oral administration of acute and sub-acute doses. Pineapple leaf extract is nontoxic, contains enzymes related to damage tissue repairing, wound healing and possibly prevents secondary infections from microbial organisms. © 2013 Elsevier Ireland Ltd. All rights reserved.

  10. Exploring of Agro Waste (Pineapple Leaf, Corn Stalk, and Napier Grass) by Chemical Composition and Morphological Study

    OpenAIRE

    Angzzas Sari Mohd Kassim; Halizah Awang; Ashuvila Mohd Aripin

    2013-01-01

    Malaysia is a country that is a rich source of agricultural waste material. Three different crops were studied here, including pineapple (Ananas comosus) leaf, corn (Zea mays) stalk, and Napier grass (Pennisetum purpureum). These crops are characterized as agricultural waste materials in Malaysia and have a high potential to be used as alternative fibers for the paper making industry. The objective of this work was to analyze the chemical composition of pineapple leaf, corn stalk, and Napier ...

  11. Optimization of lignin peroxidase, manganese peroxidase, and Lac production from Ganoderma lucidum under solid state fermentation of pineapple leaf

    OpenAIRE

    Sudha Hariharan; Padma Nambisan

    2013-01-01

    This study was undertaken to isolate ligninase-producing white-rot fungi for use in the extraction of fibre from pineapple leaf agriwaste. Fifteen fungal strains were isolated from dead tree trunks and leaf litter. Ligninolytic enzymes (lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lac)), were produced by solid-state fermentation (SSF) using pineapple leaves as the substrate. Of the isolated strains, the one showing maximum production of ligninolytic enzymes was identified...

  12. KEBERADAAN DYSMICOCCUS BREVIPES (COCKERELL (HEMIPTERA: PSEUDOCOCCIDAE SEBAGAI VEKTOR PINEAPPLE MEALYBUG WILT-ASSOCIATED VIRUS (PMWAV PADA TANAMAN NANAS

    Directory of Open Access Journals (Sweden)

    Dewi Sartiami

    2006-04-01

    Full Text Available Mealybug can almost be found in all pineapple fields (Ananas comosus (Linnaeus. The insect is known to be a vector of Pineapple Mealybug wilt-associated Virus (PMWaV. The insect samples taken from pineapple in Bunihayu, Jalancagak, Subang, West Java, were identified in laboratory. Mealybug-ant symbionts were also taken. The ability of this ant to carry the mealybugs from colony reared on kabocha (Cucurbita maxima to pineapple was also tested at green house level. Only one spesies of mealybug was found on pineapple, i.e. Dysmicoccus brevipes (Cockerell (Hemiptera: Pseudococcidae. The mealybugs were found to colonize root, basal of stem and the leaf. Eight ant species were found to be associated with mealybug. There are four species belongs to Pseudolasius genera, two species Cardiocondyla genera, Paratrechina sp. and Dorylus sp. Paratrechina sp. showed the ability to carry D. brevipes from kabocha population to pineapple. Therefore the ants should also be controlled in the total management of PMWav.

  13. Effect of UV-C radiation on bioactive compounds of pineapple (Ananas comosus L. Merr.) by-products.

    Science.gov (United States)

    Freitas, Ana; Moldão-Martins, Margarida; Costa, Helena S; Albuquerque, Tânia G; Valente, Ana; Sanches-Silva, Ana

    2015-01-01

    The industrial processing of pineapple generates a high quantity of by-products. To reduce the environmental impact of these by-products and the inherent cost of their treatment, it is important to characterise and valorise these products, converting them into high added value products. Ultra-violet radiation is one of the main sustainable sanitation techniques for fruits. Since this radiation can induce plant stress which can promote the biosynthesis of bioactive compounds, it is important to evaluate its effect in fruits. The amounts of vitamins (C and E) and carotenoids (α-carotene, β-carotene, β-cryptoxanthin, lutein, lycopene, neoxanthin, violaxanthin and zeaxanthin) in pineapple by-products (core and rind) were analysed before and after treatment with UV radiation. All treated and untreated pineapple by-products contained β-carotene as the main carotenoid (rind, 2537-3225 µg; and core, 960-994 µg 100 g(-1) DW). Pineapple rind also contained lutein (288-297 µg 100 g(-1) DW) and α-carotene (89-126 µg 100 g(-1) DW). The results provide evidence of the potential of pineapple by-products as a source of bioactive compounds with antioxidant activity, which can be used by pharmaceutical, cosmetics and food industries. In addition, UV-C was shown to be a treatment that can add nutritional value to pineapple by-products. © 2014 Society of Chemical Industry.

  14. Pineapple waste-silages as basal feed for growing Boer X Kacang cross goats

    Directory of Open Access Journals (Sweden)

    Simon P Ginting

    2007-10-01

    Full Text Available The study was aimed: 1 to evaluate the effects of using different additives on the quality of pineapple waste-silage (SLN, and 2 to investigate the responses of goats fed with PAS as a basal feed. Pineapple processing wastes include skins and the pulp left after cannery wastes are pressed to extract the juice. Six additive treatments were used in the processing of pressed pineapple wastes, namely 1 urea (5% DM, 2 Urea (2% DM and cassava meal (3% DM, 3 molasses (5% DM, 4 urea (2.5% DM and molasses (2.5% DM, 5 fermented-juice lactic acid bacteria (5% DM, and 6 without additives. Fermentation periode were set at 9, 12, 15, 18 and 21 days. The best SLN obtained from those treatments was then used in feeding trials. Twenty male Boer x Kacang crosses with an initial body weight averaging 13.2 ± 1.9 kg were used in this experiment. The animals were allocated to one of the following feed treatments, in DM: A Grasses (75% + SLN (25%, B SLN (75% + Concentrates (25%, C SLN (50% +Concentrate (50%, and D SLN (25% + Concentrates (75%. Using molasses as additive material at 5% and 15 days of fermentation period gave the best chemical and physical characteristics of the SLN. Its crude fiber content decreased and it showed the lowest pH (4.7 The silage showed temperature at 280C, and its taste was sour, and no fungi contamination. The DM and OM intakes and DM, OM and N digestibility were not different (P>0.05 between the animals fed 75% Grass/25% CON and 75% SLN/25% CON. When the proportion of concentrates in the rations was increased, the feed intake and digestibility were increase significantly (P<0.05. ADG (71.3 vs 68.8 g and feed efficiency (11.2 vs 13.4 was similar between the 75% Grass/25% CON and 75% SLN 25% CON groups. ADG increased significantly (P<0.05 when the proportion of concentrates in ration was increased to 50% (82.6 g or to 75% (89.1 g. N retention was positive in all treatments, and it was increased significantly (P<0.05 as the proportion of

  15. Calcium Ion Removal by KMnO4 Modified Pineapple Leaf Waste Carbon Prepared from Waste of Pineapple Leaf Fiber Production Processing

    Directory of Open Access Journals (Sweden)

    Sumrit Mopoung

    2016-12-01

    Full Text Available Pineapple leaf fiber waste carbon, modified with 3% KMnO4, was used for Ca2+ removal from aqueous solution. The effects of contact time, loading, water hardness, and isotherms on Ca2+ adsorption were studied. The results show that the Ca2+ ion removal by pineapple leaf fiber waste carbon could be improved by modification with KMnO4. The adsorption would reach equilibrium state at about 60 min for a water source with hardness values of 40-200 mg/dm3. Increases in total hardness (40 to 200 mg/dm3 lead to a decrease in Ca2+ ion removal efficiency (90.05% to 37.65% and an increase in Ca2+ ion adsorption capacity at equilibrium (4.37 mg/g to 8.95 mg/g. The Ca2+ removal efficiencies increase with increasing loading of modified waste carbon. The equilibrium data were fitted well by both the Langmuir isotherm and the Freundlich isotherm. For the Langmuir isotherm model, the values of the maximum Ca2+ adsorption capacity and Langmuir constant being 2.81 mg/g and 0.9262 dm3 /g, respectively. On the other hand for the Freundlich isotherm model, the KF and n values are 1.374 dm3 (1/n mg (1-1/n/g and 4.671, respectively. These results indicate that modified pineapple fiber waste carbon is a material with high Ca2+ ion adsorption capacity, heterogeneity, and high affinity.

  16. Bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions.

    Science.gov (United States)

    Vilanova Neta, Jaci Lima; da Silva Lédo, Ana; Lima, Aloisio André Bonfim; Santana, José Carlos Curvelo; Leite, Nadjma Souza; Ruzene, Denise Santos; Silva, Daniel Pereira; de Souza, Roberto Rodrigues

    2012-09-01

    The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. In this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. The most significant levels of bromelain were observed in leaf tissue after 4 months of culture in vitro in medium with a filter paper bridge, followed by medium gelled by the agar. The results of this study, regarding the different structures of the pineapple (leaves and stems) in vitro showed that the activity of bromelain varied depending on the culture conditions, the time and structure of which was quantified, ensuring a viable strategy in the production of seedlings with high levels of bromelain in subsequent phases of micropropagation.

  17. Effect of Alkali treatments on physical and Mechanical strength of Pineapple leaf fibres

    Science.gov (United States)

    Asim, M.; Jawaid, M.; Abdan, K.; Nasir, M.

    2018-01-01

    Pineapple leaf fibre (PALF) is a waste material of pineapple plants. PALF is abundant in amount for industrial purpose, cheap, easily available, high specific strength and stiffness. PALF is contributing a sustainable development in bio-composites as reinforcement material. However, natural fibres are not fully compatible with matrix due to hydrophilic in nature. To enhance the compatibility with matrix, fibres are modified its surface to make good interfacial bonding with matrix. In this research, PALF is treated with 3% and 6% concentration of NaOH for 3h, 6h 9h, and 12h soaking time. Surface modification of fibres was investigated by using scanning electron microscopy. Single fibre test and diameter of PALF fibres were evaluated the effects of NaOH treatments.

  18. Effect of the ionizing radiation in the sensorial characteristics of the pineapple

    International Nuclear Information System (INIS)

    Silva, Josenilda Maria da; Spoto, Marta Helena Fillet; Silva, Juliana Pizarro

    2007-01-01

    The post-harvest quality of the pineapple cultivar Smooth Cayenne was evaluated after the fruit was irradiated with doses of 100 and 150 Gy and stored for 10, 20 and 30 days at 12 deg C ( +-1) and relative humidity of 85% (+- 5). The controls were untreated fruits. Sensorial analyses were made for each storage period to obtain information about the effects of ionizing radiation on the quality of the fruit. The ionizing radiation dosage had little effect on the sensorial characteristics of the pineapple, although the best results were obtained with fruit irradiated with 100 Gy. The 20-day storage period resulted in the highest consumer acceptability, while the 30-day storage period impaired the fruit's external and internal appearance. (author)

  19. Cloning of the queen variety pineapple (Ananas comosus (L.) merr) through the callus

    International Nuclear Information System (INIS)

    Lapade, A.G.; Veluz, A.M.S.; Santos, I.S.

    1988-01-01

    Crown sections of pineapple were inoculated aseptically in Murashige and Skoog (MS) medium with varying levels of benzyl adenine (BA) in combination with napthalene acetic acid (NAA). Callus was induced from crown sections that was grown in MS with 0 ppm BA + 2 ppm NAA, 2 ppm BA + 6 ppm NAA. These calli when grown continuously in the same medium developed profusely into shoots. Callus and shoots developed profusely at medium containing 2 ppm BA + 2 ppm NAA. Calli that were further sub-cultured in MS with 2 ppm BA + 2 ppm NAA produced multiple shoots. Calli and shoots that were further sub-cultured in 0 to 4 ppm BA to 4 to 6 ppm NAA formed roots. Results showed that complete plantlets were produced from crown sections of pineapple variety Queen in MS medium supplemented with the different treatment combinations of BA and NAA. (Author). 9 refs.; 1 fig

  20. Polyphenol oxidase and peroxidase expression in four pineapple varieties (Ananas comosus L.) after a chilling injury.

    Science.gov (United States)

    Raimbault, Astrid-Kim; Marie-Alphonsine, Paul-Alex; Horry, Jean-Pierre; Francois-Haugrin, Madlyn; Romuald, Karell; Soler, Alain

    2011-01-12

    Pineapple internal browning (IB) is a chilling injury that produces enzymatic browning associated with flesh translucency. Pineapple biodiversity allowed the investigation of how polyphenol oxidase (PPO) and peroxidase (POD) activities with their different isoforms are involved in the IB mechanism. Fruits of four varieties that expressed IB symptoms differently, Smooth Cayenne (SCay) and the hybrids MD2, Flhoran 41 (Flh 41), and Flhoran 53 (Flh 53), were stressed by cold. The susceptible varieties showed classical brown spots but different patterns of IB, whereas MD2 and controls showed no IB. Enzymatic activities were measured on fruit protein extracts and PPO and POD isoforms separated on mini-gels (PhastSystem). Only PPO activity was significantly enhanced in the presence of IB. Up to six PPO isoforms were identified in the susceptible varieties. PPO was barely detectable in the nonsusceptible variety MD2 and in controls. The number of PPO isoforms and the total PPO activity after chilling are varietal characteristics.

  1. Chemical pulping of waste pineapple leaves fiber for kraft paper production

    Directory of Open Access Journals (Sweden)

    Waham Ashaier Laftah

    2015-07-01

    Full Text Available The main objective of this study is to evaluate the implementation of acetone as a pulping agent for pineapple leaves. Mixtures of water and acetone with concentration of 1%, 3%, 5%, 7%, and 10% were used. The effects of soaking and delignification time on the paper properties were investigated. Thermal and physical properties of paper sheet were studied using thermogravimetric analysis (TGA and tearing resistance test respectively. The morphological properties were observed using microscope at 200× magnification. The paper sheet produced from pulping with 3% acetone concentration shows the highest mechanical properties. Papers strength was improved by increasing the delignification time. The delignification time was reduced by cooking the pineapple leaves at a temperature of 118 °C under applied pressure of 80 kPa which has remarkable effect on paper strength.

  2. Empirical Modeling on Hot Air Drying of Fresh and Pre-treated Pineapples

    Directory of Open Access Journals (Sweden)

    Tanongkankit Yardfon

    2016-01-01

    Full Text Available This research was aimed to study drying kinetics and determine empirical model of fresh pineapple and pre-treated pineapple with sucrose solution at different concentrations during drying. 3 mm thick samples were immersed into 30, 40 and 50 Brix of sucrose solution before hot air drying at temperatures of 60, 70 and 80°C. The empirical models to predict the drying kinetics were investigated. The results showed that the moisture content decreased when increasing the drying temperatures and times. Increase in sucrose concentration led to longer drying time. According to the statistical values of the highest coefficients (R2, the lowest least of chi-square (χ2 and root mean square error (RMSE, Logarithmic model was the best models for describing the drying behavior of soaked samples into 30, 40 and 50 Brix of sucrose solution.

  3. Determination of Cd and Pb in canned pineapple by atomic absorption spectroscopy using a graphite oven

    International Nuclear Information System (INIS)

    Linares P, G.; Sanchez P, L.; Benavides M, A.; Acosta L, C.

    1997-01-01

    Owing to the food susceptibility to be contaminated it is necessary to realize pursuit studies or frequent monitoring about the content of certain metals which represent health risks by its toxicological effects in the human being and another living organisms. In this work the cadmium and lead concentrations are determined in six national brands of canned pineapple analysing separately sugar sirup and the fruit. (Author)

  4. Performance of pineapple slips inoculated with diazotrophic phosphate-solubilizing bacteria and rock phosphate

    OpenAIRE

    Lílian Estrela Borges Baldotto; Marihus Altoé Baldotto; Fábio Lopes Olivares; Adriane Nunes de Souza

    2014-01-01

    Besides fixing N2, some diazotrophic bacteria or diazotrophs, also synthesize organic acids and are able to solubilize rock phosphates, increasing the availability of P for plants. The application of these bacteria to pineapple leaf axils in combination with rock phosphate could increase N and P availability for the crop, due to the bacterial activity of biological nitrogen fixation and phosphate solubilization. The objectives of this study were: (i) to select and characterize diazotrophs abl...

  5. Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

    OpenAIRE

    Silva,Aurilena de Aviz; Carvalho,Almy Junior Cordeiro de; Freitas,Flávia Paiva de; Pessanha,Patrícia Gomes de Oliveira; Santos,Paulo Cesar dos; Silva,Mírian Peixoto Soares da; Vasconcelos,Tábatha de Souza; Olivares,Fábio Lopes

    2016-01-01

    ABSTRACT: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholder...

  6. Genomic Survey, Characterization, and Expression Profile Analysis of the SBP Genes in Pineapple (Ananas comosus L.).

    Science.gov (United States)

    Ali, Hina; Liu, Yanhui; Azam, Syed Muhammad; Rahman, Zia Ur; Priyadarshani, S V G N; Li, Weimin; Huang, Xinyu; Hu, Bingyan; Xiong, Junjie; Ali, Umair; Qin, Yuan

    2017-01-01

    Gene expression is regulated by transcription factors, which play many significant developmental processes. SQUAMOSA promoter-binding proteins (SBP) perform a variety of regulatory functions in leaf, flower, and fruit development, plant architecture, and sporogenesis. 16 SBP genes were identified in pineapple and were divided into four groups on basis of phylogenetic analysis. Five paralogs in pineapple for SBP genes were identified with Ka/Ks ratio varied from 0.20 for AcSBP14 and AcSBP15 to 0.36 for AcSBP6 and AcSBP16 , respectively. 16 SBP genes were located on 12 chromosomes out of 25 pineapple chromosomes with highly conserved protein sequence structures. The isoionic points of SBP ranged from 6.05 to 9.57, while molecular weight varied from 22.7 to 121.9 kD. Expression profiles of SBP genes revealed that AcSBP7 and AcSBP15 (leaf), AcSBP13 , AcSBP12 , AcSBP8 , AcSBP16 , AcSBP9 , and AcSBP11 (sepal), AcSBP6 , AcSBP4 , and AcSBP10 (stamen), AcSBP14 , AcSBP1 , and AcSBP5 (fruit) while the rest of genes showed low expression in studied tissues. Four genes, that is, AcSBP11 , AcSBP6 , AcSBP4 , and AcSBP12 , were highly expressed at 4°C, while AcSBP16 were upregulated at 45°C. RNA-Seq was validated through qRT-PCR for some genes. Salt stress-induced expression of two genes, that is, AcSBP7 and AcSBP14 , while in drought stress, AcSBP12 and AcSBP15 were highly expressed. Our study lays a foundation for further gene function and expression studies of SBP genes in pineapple.

  7. Surmounting self-incompatibility in pineapple (Ananas comosus L.) with pollen irradiation

    International Nuclear Information System (INIS)

    Subramanian, N.; Iyer, C.P.A.; Singh, Rajendra

    1981-01-01

    Irradiation was investigated as a tool to overcome self-incompatibility in pineapple. Pollen grains were irradiated with 15, 20, 25 kr gamma radiation from cobalt-60 and were used for pollination on the stigma of the same inflorescence. Observations on seed-set were made when the fruits were ripe. It was observed that 20 kr gamma radiation was effective in overcoming self-incompatibility. (M.G.B.)

  8. Heterogeneity in pineapple fruit quality results from plant heterogeneity at flower induction.

    Science.gov (United States)

    Fassinou Hotegni, V Nicodème; Lommen, Willemien J M; Agbossou, Euloge K; Struik, Paul C

    2014-01-01

    Heterogeneity in fruit quality constitutes a major constraint in agri-food chains. In this paper the sources of the heterogeneity in pineapple in the field were studied in four experiments in commercial pineapple fields. The aims were to determine (a) whether differences in pineapple fruit quality among individual fruits are associated with differences in vigor of the individual plants within the crop at the time of artificial flower induction; and (b) whether the side shoots produced by the plant during the generative phase account for the fruit quality heterogeneity. Two pineapple cultivars were considered: cv. Sugarloaf and cv. Smooth Cayenne. Plant vigor at the time of artificial flower induction was measured by three variates: the number of functional leaves, the D-leaf length and their cross product. Fruit quality attributes measured at harvest time included external attributes (weight and height of fruit, infructescence and crown) and internal quality attributes [total soluble solids (TSS), pH, translucent flesh]. Results showed that the heterogeneity in fruit weight was a consequence of the heterogeneity in vigor of the plants at the moment of flower induction; that effect was mainly on the infructescence weight and less or not on the crown weight. The associations between plant vigor variates at flower induction and the internal quality attributes of the fruit were poor and/or not consistent across experiments. The weight of the slips (side shoots) explained part of the heterogeneity in fruit weight, infructescence weight and fruit height in cv. Sugarloaf. Possibilities for reducing the variation in fruit quality by precise cultural practices are discussed.

  9. Heterogeneity in pineapple fruit quality results from plant heterogeneity at flower induction

    Directory of Open Access Journals (Sweden)

    V. Nicodeme eFassinou Hotegni

    2014-12-01

    Full Text Available Heterogeneity in fruit quality constitutes a major constraint in agri-food chains. In this paper the sources of the heterogeneity in pineapple in the field were studied in four experiments in commercial pineapple fields. The aims were to determine (a whether differences in pineapple fruit quality among individual fruits are associated with differences in vigor of the individual plants within the crop at the time of artificial flower induction; and (b whether the side shoots produced by the plant during the generative phase account for the fruit quality heterogeneity. Two pineapple cultivars were considered: cv. Sugarloaf and cv. Smooth Cayenne. Plant vigor at the time of artificial flower induction was measured by three variates: the number of functional leaves, the D-leaf length and their cross product. Fruit quality attributes measured at harvest time included external attributes (weight and height of fruit, infructescence and crown and internal quality attributes (total soluble solids, pH, translucent flesh. Results showed that the heterogeneity in fruit weight was a consequence of the heterogeneity in vigor of the plants at the moment of flower induction; that effect was mainly on the infructescence weight and less or not on the crown weight. The association between plant vigor variates at flower induction and the internal quality attributes of the fruit were poor and/or not consistent across experiments. The weight of the slips (side shoots, explained part of the heterogeneity in fruit weight, infructescence weight and fruit height in cv. Sugarloaf. Possibilities for reducing the variation in fruit quality by precise cultural practices are discussed.

  10. Chemical pulping of waste pineapple leaves fiber for kraft paper production

    OpenAIRE

    Laftah, Waham Ashaier; Abdul Rahaman, Wan Aizan Wan

    2015-01-01

    The main objective of this study is to evaluate the implementation of acetone as a pulping agent for pineapple leaves. Mixtures of water and acetone with concentration of 1%, 3%, 5%, 7%, and 10% were used. The effects of soaking and delignification time on the paper properties were investigated. Thermal and physical properties of paper sheet were studied using thermogravimetric analysis (TGA) and tearing resistance test respectively. The morphological properties were observed using microscope...

  11. Comparison of in vivo toxicity, antioxidant and immunomodulatory activities of coconut, nipah and pineapple juice vinegars.

    Science.gov (United States)

    Mohamad, Nurul Elyani; Keong Yeap, Swee; Beh, Boon Keen; Romli, Muhammad Firdaus; Yusof, Hamidah Mohd; Kristeen-Teo, Ye Wen; Sharifuddin, Shaiful Adzni; Long, Kamariah; Alitheen, Noorjahan Banu

    2018-01-01

    Vinegar is widely used as a food additive, in food preparation and as a food supplement. This study compared the phenolic acid profiles and in vivo toxicities, and antioxidant and immunomodulatory effects of coconut, nipah and pineapple juice vinegars, which were respectively prepared via a two-step fermentation using Saccharomyces cerevisiae 7013 INRA and Acetobacter aceti vat Europeans. Pineapple juice vinegar, which had the highest total phenolic acid content, also exhibited the greatest in vitro antioxidant capacity compared to coconut juice and nipah juice vinegars. Following acute and sub-chronic in vivo toxicity evaluation, no toxicity and mortality were evident and there were no significant differences in the serum biochemical profiles between mice administered the vinegars versus the control group. In the sub-chronic toxicity evaluation, the highest liver antioxidant levels were found in mice fed with pineapple juice vinegar, followed by coconut juice and nipah juice vinegars. However, compared to the pineapple juice and nipah juice vinegars, the mice fed with coconut juice vinegar, exhibited a higher population of CD4 + and CD8 + T-lymphocytes in the spleen, which was associated with greater levels of serum interleukin-2 and interferon-γ cytokines. Overall, the data suggested that not all vinegar samples cause acute and sub-chronic toxicity in vivo. Moreover, the in vivo immunity and organ antioxidant levels were enhanced, to varying extents, by the phenolic acids present in the vinegars. The results obtained in this study provide appropriate guidelines for further in vivo bioactivity studies and pre-clinical assessments of vinegar consumption. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  12. Effect of pineapple waste powder on probiotic growth, antioxidant and antimutagenic activities of yogurt.

    Science.gov (United States)

    Sah, B N P; Vasiljevic, T; McKechnie, S; Donkor, O N

    2016-03-01

    Although many fruit by-products are good sources of nutrients, little is known about their prebiotic potential. This research was aimed at establishing the prebiotic effect of pineapple wastes on probiotics including Lactobacillus (L.) acidophilus (ATCC® 4356™), L. casei (ATCC® 393™) and L. paracasei spp. paracasei (ATCC® BAA52™) and the subsequent release of antioxidant and antimutagenic peptides in yogurt during their growth. Oven- and freeze- dried peel and pomace were milled separately into powders and tested for prebiotic activities. The net probiotic growth (1.28-2.14 log cfu/g) in customized MRS broth containing the pineapple powders as a direct carbohydrate source was comparable to MRS broth containing glucose. The powders were also separately added to milk during the manufacturing of yogurt with or without probiotics. An increase (by 0.3-1.4 log cycle) in probiotic populations was observed in the yogurts as a consequence of pineapple powder supplementation. Crude water-soluble peptide extracts, prepared by high-speed centrifugation of the yogurts, displayed remarkable antioxidant activities assessed through in vitro assays, namely scavenging activity of 1,1-diphenyl-2-picrylhydrazyl radicals (IC50 = 0.37-0.19 mg/ml) and hydroxyl radicals (58.52-73.55 %). The peptide extracts also exhibited antimutagenic activities (18.60-32.72 %) as sodium azide inhibitor in the Salmonella mutagenicity test. Together, these results suggest that pineapple by-products exhibited prebiotic properties and could possibly be commercially applied in new functional food formulations.

  13. Bioprospecting of powdered pineapple rind as an organic supplement of composted sawdust for Pleurotus ostreatus mushroom cultivation.

    Science.gov (United States)

    Narh Mensah, Deborah L; Addo, Peter; Dzomeku, Matilda; Obodai, Mary

    2018-03-01

    Pineapple rind is a by-product of the pineapple processing industry and contains nutrients and other compounds which must be utilized as a bioresource for socio-economic benefits while preventing the potential problems of improper agroindustrial biomass disposal methods. Pleurotus ostreatus is an edible oyster mushroom with medicinal properties and can be cultivated on various agroindustrial biomass, including sawdust containing supplements. Pineapple rind was powdered and used as a supplement of composted sawdust at 2%, 5%, 10%, 12%, 15%, and 20% (w/w) on dry weight basis. A control treatment consisted of composted sawdust supplemented with rice bran at 12% (the most utilized composition in Ghana). P. ostreatus strain EM-1 was cultivated on these treatments. Factors investigated included the spawn run period, yield, fruiting body weight and size, biological efficiency, and nutritional composition (proximate composition and Copper, Zinc and Lead content) of fruiting bodies harvested from selected high-yielding treatments and the control treatment. Full colonization of all treatments occurred by the 34th day of incubation. Enhanced yield, fruiting body weight and size, and biological efficiency were generally recorded with supplementation at lower concentrations (2% and 5%) compared to treatments supplemented at higher concentrations. There was also a supplement concentration-dependent alteration of the nutritional composition of the mushroom. Powdered pineapple rind can be utilized as an organic supplement at relatively low concentrations in composted sawdust for P. ostreatus strain EM-1 cultivation. The use of lower concentrations of powdered pineapple rind in composted sawdust is advantageous as relatively less input will be required to produce higher P. ostreatus strain EM-1 yields. Utilization of pineapple rind for mushroom cultivation will extend the pineapple plant value chain, intensify mushroom production in a sustainable way, and minimize agricultural

  14. Plant mutation breeding of pineapple (Ananas comosus (L.) Merr.) using gamma irradiation for improvement of smooth cayenne variety

    International Nuclear Information System (INIS)

    Soeranto Human; S Loekito; M Trilaksono; A Syaifudin

    2016-01-01

    Currently, the most famous pineapple cultivar cultivated for the world trade is Smooth Cayenne. Many clones derived from this cultivar such as GP1, GP2, GP3, GP4, GP5, and F180 are grown by GGPC for fresh and processed fruits. GGPC started pineapple breeding and varietal improvement programs in 1986 with the objectives to increase quality, tonage and yield. Mutation breeding in pineapple was started in 2006 i.e. in collaboration with the Center for Isotopes and Radiation Application (CIRA), the National Nuclear Energy Agency (BATAN). A number of 10 pineapple crowns originated from GP2, GP3 (A10) and F180 clones were treated with gamma irradiation from Cobalt-60 source installed in gamma chamber 4000 A, using the doses of 200 and 300 Gy. The irradiated crowns were then planted in the experimental field (as V1) and maintained following the GGPC commercial standard cultivation for pineapple. The results showed there was no significant differences between the two gamma irradiation doses (200 and 300 Gy) on pineapple phenotypic performances. However, high phenotypic variability was found in clones at the second vegetative propagation (V2). Some plant variations were recorded as follows: 47 % of normal vigour, 15 % of rosset, 11 % of spiny, 5 % of crowns with double tips, 4 % of plant having plenty of leaves and 18 % of fruits with abnormal shape. Significant mutant variation was also observed in clones the third vegetative propagation (V3) but some mutants seemed to be more stable in the V3 generation. This pineapple mutation breeding program will be continued for mutant evaluation that is related to improvement of productivity, quality and resistance to major insect and diseases. (author)

  15. Production, characterization and acceptability of pineapple liqueurs prepared with different alcoholic bases

    Directory of Open Access Journals (Sweden)

    Érica Resende Oliveira

    2015-04-01

    Full Text Available Evaluation of physico-chemical parameters and the acceptability of liquors pineapple (Ananas comosus L. Merri, produced with different alcoholic bases: rum, flavored with pineapple peel and rum doubly distilled grain alcohol, were performed in this study. We conducted analyzes of alcoholic strength, density, pH, soluble solids, total fixed and volatile acidity, dry, and sensory analysis through the affective test of acceptability with 100 judges using 9-point hedonic scale to evaluate the attributes color, flavor , odor and overall impression, and purchase intent test. The average alcohol content found for all treatments was 25% v / v, a value that lies within the limits of the Brazilian law. The values of dry matter, fixed acidity, density and L * is not distinguished between treatments. The color parameters L *, a *, b *, chroma and H °, and the results of the analysis of the total acidity, soluble solid showed up only statistically different for liquor-based grain alcohol. The average pH differed for rum-based liqueur. All liquors showed good acceptability, averaging above 6 and 49% of the panelists claimed that the product would probably buy pineapple liqueur made with different alcoholic bases. The liquor made of only rum was the most accepted as the global average of items analyzed.

  16. Growth, production and quality of pineapple in response to herbicide use

    Directory of Open Access Journals (Sweden)

    Leonardo Carvalho Brant Maia

    2012-09-01

    Full Text Available In pineapple fields, weed competition is exacerbated by the fact that the crop is small and has a very slow vegetative development. The objective of this study was to determine the effects of herbicides on growth, yield and quality of pineapple, cultivar 'Pérola'. The experimental design was in randomized blocks with four treatments and four replications. Treatments consisted of weeding by hoe and the herbicides diuron; fluazifop-p-butyl and atrazine + S-metolachlor applied in post-emergence. The characteristics evaluated monthly during the vegetative stage were stem diameter, D-leaf length, number of leaves, number of emitted leaves and percentage of natural floral induction. In the reproductive phase, evaluations were made of average fruit weight (g with and without crown, fruits length and diameter, number of slip, slip-sucker and sucker type seedlings, determination of soluble solids and pH in the pulp. There was no effect of herbicide treatment on the vegetative growth characteristics. Stem diameter increased until 330 days after planting, showing a decrease after this period. The D-leaf grew over time in all treatments, although phytotoxicity symptoms were observed after the first application of herbicides. The traits evaluated on the reproductive phase showed no significant differences in response to treatments. Therefore, the use of diuron fluazifop-p-butyl and atrazine + S-metolachlor did not affect growth, yield and fruit quality of pineapple, cultivar 'Pérola'.

  17. THE ANALYSIS OF AGRICULTURAL PRODUCT IN PINEAPPLE COMMODITIES IN NGANCAR DISTRICT, KEDIRI REGENCY, EAST JAVA PROVINCE

    Directory of Open Access Journals (Sweden)

    Risdiana Himmati

    2017-05-01

    Full Text Available The present research has the purpose to analyze factors affecting the production of pineapple in Ngancar District, Kediri Regency, East Java Province. The research took place in Ngancar District, Kediri Regency, East Java Province, employing secondary data derived from Central Bureau of Statistics (Kediri Regency and Agricultural Extension Agency (Ngancar District and primary data consisted of farming production data, land area, amount of seeds, and amount of fertilizer usage, pesticide and workforce. The analysis technique used in this research was Cobb-Douglas production function. The completion upon Cobb-Douglas production functions used Ordinary Least Square (OLS method with Eviews 9 program tool. The sampling technique employed in this research was simple random sampling method. The results demonstrate that the pineapple total production in Ngancar District is affected by land area, amount of seeds, molasses, and urea. The research concludes that pineapple production in Ngancar District is affected by all the factors of production, except the usage of pesticide and workforce.

  18. Influence of smoke and ethylene on the fruiting of the pineapple (Ananas sativus Shult

    Energy Technology Data Exchange (ETDEWEB)

    Rodriguez, A G

    1932-01-01

    The use of smoke to force pineapple plants into fruit production is a practical development. No controlled experimental work has been reported of this practice. For this reason it seemed desirable to determine the influence of smoke under controlled conditions. In considering the cause of flowering of the pineapple plant following the treatment of smoke, the primary hypothesis raised was that smoke forces the pineapple plant out of a dormant condition. However it was recognized that some constituents of smoke probably ethylene might effect metabolic changes even though the plants were not in a dormant condition, which changes might conduce to floral development. Both slips and suckers are completely developed plants. The roots at the time of planting are very short. The fact that new leaf development is retarded four to six weeks after planting suggests that slips and suckers are in a state of dormancy. It seemed probable that the use of ethylene on these might bring about an immediate resumption on growth with a consequent shortening of the time for flower production. There also remained the possibility as was realized, of a more pronounced effect of ethylene on fruit production. Accordingly an experiment was devised in which both slips and suckers were exposed to ethylene previous to planting. Such a method, if it proved successful, would obviously be a cheaper and more practical method than the use of smoke under field conditions.

  19. Performance of pineapple slips inoculated with diazotrophic phosphate-solubilizing bacteria and rock phosphate

    Directory of Open Access Journals (Sweden)

    Lílian Estrela Borges Baldotto

    2014-06-01

    Full Text Available Besides fixing N2, some diazotrophic bacteria or diazotrophs, also synthesize organic acids and are able to solubilize rock phosphates, increasing the availability of P for plants. The application of these bacteria to pineapple leaf axils in combination with rock phosphate could increase N and P availability for the crop, due to the bacterial activity of biological nitrogen fixation and phosphate solubilization. The objectives of this study were: (i to select and characterize diazotrophs able to solubilize phosphates in vitro and (ii evaluate the initial performance of the pineapple cultivars Imperial and Pérola in response to inoculation with selected bacteria in combination with rock phosphate. The experiments were conducted at Universidade Estadual do Norte Fluminense Darcy Ribeiro, in 2009. In the treatments with bacteria the leaf contents of N, P and K were higher than those of the controls, followed by an increase in plant growth. These results indicate that the combined application of diazotrophic phosphate-solubilizing bacteria Burkholderia together with Araxá rock phosphate can be used to improve the initial performance of pineapple slips.

  20. Pineapple leaf fiber as reinforce in composite materials, an alternative for automotive industry

    International Nuclear Information System (INIS)

    Campos, Rejane Daniela de; Seo, Emilia Satoshi Miyamaru

    2013-01-01

    The composites appear as an extremely favorable alternative for different industries, due to the fact that it combines the best mechanical properties with the best physic-chemical properties of two or more materials. Nowadays, in the evaluation of materials, besides criteria such as economic viability and performance, the environmental criterion was included in this evaluation. Part of the environmental criteria is the use of biodegradable materials and/or recycled materials. In this sense, researches focused on vegetal fibers, as reinforcement in composites are growing considerably and positive results for its performance were achieved. Moreover, the environmental-friendly approach not only is the unique advantage on usage of vegetal fibers, but also it has an economical advantage, because of the low cost and good performance due to low density. The fiber extracted from the pineapple leaf (PALF) is a new alternative for automotive industry as cellulose-based fiber composite. In this sense, the present paper aims to present the characterization of the pineapple leaf fiber for manufacturing the automotive composite materials. Milled pineapple fibers extracted, in two different ways and submitted to mercerisation treatments, were characterized by mechanical and thermal properties; density; morphology; FTIR spectroscopy, EDX and X-ray diffraction. It is important to characterize the fibers, in order to obtain appropriate mechanical properties of composite. (author)

  1. Study on evaluation of silage from pineapple (Ananas comosus) fruit residue as livestock feed.

    Science.gov (United States)

    Gowda, Nisarani Kollurappa Shivakumar; Vallesha, Naglapura Chandrashekara; Awachat, Vaibhav Bhagvan; Anandan, Samireddypalli; Pal, Din Taran; Prasad, Cadaba Srinivasa

    2015-03-01

    Pineapple is a commercially important fruit crop grown in Asian and African countries. Pineapple fruit residue (PFR) accounts for more than 65% of the processed fruits, and its disposal is a major problem due to its high moisture and sugar content predisposing it to fungal growth and spoilage. Silage technique was adopted to address this problem, and the PFR silage was evaluated for its feeding value. It was observed that on 15th day, the pH of PFR silage was 4.2-4.3 and lactic acid content was 6-8% (DM basis). Combination of 4 parts leafy crown and 1 part peels/pomace was found very ideal to achieve moisture content of 65-70% and produced a good quality silage with minimum fungal count (Pineapple fruit residue that was hitherto wasted was successfully converted to silage and was found to be a valuable alternative to conventional green fodder. Ensiling of PFR not only improved the economics of feeding but also helped in overcoming the disposal problem.

  2. Pineapple leaf fiber as reinforce in composite materials, an alternative for automotive industry

    Energy Technology Data Exchange (ETDEWEB)

    Campos, Rejane Daniela de; Seo, Emilia Satoshi Miyamaru, E-mail: rejanedaniela@ipen.br [Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)

    2013-07-01

    The composites appear as an extremely favorable alternative for different industries, due to the fact that it combines the best mechanical properties with the best physic-chemical properties of two or more materials. Nowadays, in the evaluation of materials, besides criteria such as economic viability and performance, the environmental criterion was included in this evaluation. Part of the environmental criteria is the use of biodegradable materials and/or recycled materials. In this sense, researches focused on vegetal fibers, as reinforcement in composites are growing considerably and positive results for its performance were achieved. Moreover, the environmental-friendly approach not only is the unique advantage on usage of vegetal fibers, but also it has an economical advantage, because of the low cost and good performance due to low density. The fiber extracted from the pineapple leaf (PALF) is a new alternative for automotive industry as cellulose-based fiber composite. In this sense, the present paper aims to present the characterization of the pineapple leaf fiber for manufacturing the automotive composite materials. Milled pineapple fibers extracted, in two different ways and submitted to mercerisation treatments, were characterized by mechanical and thermal properties; density; morphology; FTIR spectroscopy, EDX and X-ray diffraction. It is important to characterize the fibers, in order to obtain appropriate mechanical properties of composite. (author)

  3. Fabrication, mechanical characterization of pineapple leaf fiber (PALF) reinforced vinylester hybrid composites

    Science.gov (United States)

    Yogesh, M.; Rao, A. N. Hari

    2018-04-01

    Natural fibre based composites are under intensive study due to their eco friendly nature and peculiar properties. The advantage of natural fibres is their continuous supply, easy and safe handling, and biodegradable nature. Although natural fibres exhibit admirable physical and mechanical properties, it varies with the plant source, species, geography, and so forth. Pineapple leave fibre (PALF) is one of the abundantly available waste materials in India and has not been studied yet. The work has been carried out to fabrication and study the mechanical characterization of Pineapple Leaf fiber reinforced Vinylester composites filled with different particulate fillers. These results are compared with those of a similar set of glass fiber reinforced Vinylester composites filled with same particulate fillers. It is evident that the density values for Pineapple leaf fiber (PALF) - Vinylester composites increase with the particulate filler content and void fractions in these composites also increase. The test results show that with the presence of particulate fillers, micro hardness of the PALF-Vinylester composites has improved. Among all the composites under this investigation, the maximum hardness value is recorded for PALF-Vinylester composite filled with 20 wt% alumina. In this investigation the maximum value of ILSS has been recorded for the PALF-Vinylester composite with 20 wt% of Flyash.

  4. Characterization of a polyhydroxyalkanoate obtained from pineapple peel waste using Ralsthonia eutropha.

    Science.gov (United States)

    Vega-Castro, Oscar; Contreras-Calderon, Jose; León, Emilson; Segura, Almir; Arias, Mario; Pérez, León; Sobral, Paulo J A

    2016-08-10

    Agro-industrial waste can be the production source of biopolymers such as polyhydroxyalkanoates. The aim of this study was to produce and characterize Polyhydroxyalkanoates produced from pineapple peel waste fermentation processes. The methodology includes different pineapple peel waste fermentation conditions. The produced biopolymer was characterized using FTIR, GC-MS and NMR. The best fermentation condition for biopolymer production was obtained using pH 9, Carbon/Nitrogen 11, carbon/phosphorus 6 and fermentation time of 60h. FTIR analyzes showed PHB group characteristics, such as OH, CH and CO. In addition, GC-MS showed two monomers with 4 and 8 carbons, referred to PHB and PHBHV. H(1) NMR analysis showed 0.88-0.97 and 5.27ppm signals, corresponding to CH3 and CH, respectively. In conclusion, polyhydroxyalkanoate production from pineapple peels waste is an alternative for the treatment of waste generated in Colombia's fruit industry. Copyright © 2016 Elsevier B.V. All rights reserved.

  5. Survival of the endangered Pima pineapple cactus: Does clearing before prescribed fire alter survival postfire?

    Science.gov (United States)

    Thomas, Kathryn A.; Jarchow, Christopher; Crawford, Julie A.

    2017-01-01

    Federal land managers and ranchers often use prescribed fire as a tool to reduce invading woody plants within desert grasslands of the arid southwestern United States. Managers must evaluate the threat of the burn toward the health and survival of plants of concern including how preemptive clearing before prescribed fire might benefit these species. One example is the endangered Pima pineapple cactus (Coryphantha scheeri var. robustispina), a small hemispheric cactus of desert scrublands and grasslands of south-central Arizona and northern Sonora, Mexico. In 2014, we examined survival of Pima pineapple cactus documented in 2009 or 2010 within grasslands of Buenos Aires National Wildlife Refuge in Arizona. Of the 72 sites observed, 35 had no burn after documentation and 37 experienced prescribed fire. Refuge staff removed vegetation between 0.3 and 3.0 m from the cactus preburn. We found that Pima pineapple cacti in areas subjected to prescribed fire and with preemptive clearing had the same survival statistically as cacti from sites that were not burned.

  6. Sustainable biobutanol production from pineapple waste by using Clostridium acetobutylicum B 527: Drying kinetics study.

    Science.gov (United States)

    Khedkar, Manisha A; Nimbalkar, Pranhita R; Gaikwad, Shashank G; Chavan, Prakash V; Bankar, Sandip B

    2017-02-01

    Present investigation explores the use of pineapple peel, a food industry waste, for acetone-butanol-ethanol (ABE) production using Clostridium acetobutylicum B 527. Proximate analysis of pineapple peel shows that it contains 35% cellulose, 19% hemicellulose, and 16% lignin on dry basis. Drying experiments on pineapple peel waste were carried out in the temperature range of 60-120°C and experimental drying data was modeled using moisture diffusion control model to study its effect on ABE production. The production of ABE was further accomplished via acid hydrolysis, detoxification, and fermentation process. Maximum total sugar release obtained by using acid hydrolysis was 97g/L with 95-97% and 10-50% removal of phenolics and acetic acid, respectively during detoxification process. The maximum ABE titer obtained was 5.23g/L with 55.6% substrate consumption when samples dried at 120°C were used as a substrate (after detoxification). Copyright © 2016 Elsevier Ltd. All rights reserved.

  7. Potassium fertilization for pineapple: effects on soil chemical properties and plant nutrition

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Junqueira Teixeira

    2011-06-01

    Full Text Available A field experiment was carried out on an Ultisol located at the city of Agudos (22º30'S; 49º03'W, in the state of São Paulo, Brazil, in order to determine the effects of rates and sources of potassium fertilizer on nutritional status of 'Smooth Cayenne' pineapple and on some soil chemical properties. The experiment was a complete factorial design with four rates (0, 175, 350, and 700 kg ha-1 of K2O and three combinations of K sources (100% KCl, 100% K2SO4 and 40% K2SO4 + 60% KCl. Soil samples were taken from the depths 0-20 cm, 20-40 cm and 40-60 cm at planting and 14 months after. Nutritional status of pineapple plants was assessed by means of tissue analysis. Soil K availability increased with application of K fertilizer, regardless of K sources. Soil chlorine and Cl concentration in pineapple leaves increased with application of KCl or K2SO4+KCl. Plant uptake of potassium was shaped by soil K availability and by the application rates of K fertilizer, independently of K sources.

  8. Water dephts and macronutrients accumulation in 'pérola' pineapple irrigated by drip

    Directory of Open Access Journals (Sweden)

    Uirá do Amaral

    2014-09-01

    Full Text Available Brazil is one of the largest pineapple producers worldwide, and those fruits are for the juice industry and the in natura market. Its cultivation requires high technology investment by using irrigation and balanced fertilization. However, little is known about the influence of drip irrigation on nutrient uptake by pineapple plants. This study aimed to evaluate the influence of different irrigation depths on nutrients accumulation by 'Pérola' pineapples grown in northern region of Minas Gerais State, Brazil. The experimental design was in randomized blocks with five treatments referring to: 30% of Class A Evaporation Pan (ECA; 50% of ECA; 70% of ECA; 100% of ECA; and 150% of ECA. To determine the levels of macronutrients and dry matter, the plants were separated in root, stem, leaves, 'D' leaf, crown, fruit and whole plant. The sequence of macronutrients accumulated in the whole plant was K>N>Ca>P>Mg. The fruits exported from the cultivated area the following amounts of macronutrients: 17.52 kg ha-1 of K (12.8%; 16.91 kg ha-1 of N (20.7%; 10.77 kg ha-1 of Ca (15.9%, 1.29 kg ha-1 of P (12.4% and 1.04 kg ha-1 of Mg (20.5%. The irrigation depths that provided the maximum N, P, K and Ca accumulation in the whole plant are 53.6, 61.6, 54.5 and 60.2% of ECA, respectively.

  9. Clonal evaluation of new ornamental pineapple hybrids to use as cut flowers

    Directory of Open Access Journals (Sweden)

    Davi Silva Costa Junior

    2016-09-01

    Full Text Available Embrapa Cassava and Fruits has developed ornamental pineapple hybrids for several categories of ornamental use including cut flowers, potted plants and landscaping. The objective of this study was to carry out a clonal evaluation of two ornamental hybrids using quantitative and qualitative morphological descriptors to recommend these hybrids as new ornamental pineapple cultivars. Twenty plants of each hybrid (PL01 and PL04 were evaluated regarding the response to floral induction as well as the stability and homogeneity of the clones in two production cycles. The descriptive statistics were calculated and analyzed to determine the genetic distance based on the Gower algorithm. Four groups were formed, two using parental data and the other two containing the different hybrids that were evaluated in the two growing cycles. In the floral evaluation, the time from field planting to harvest of the stem as a cut flower was determined to be as long as 17 months in the first cycle and 13.5 months in the second cycle for both hybrids. They were characterized as a novelty in the flower market; they showed genetic stability and homogeneity and can be recommended as new cultivars of ornamental pineapple because they exhibit satisfactory quality and meet the market requirements.

  10. Concentration of pineapple juice by reverse osmosis: physicochemical characteristics and consumer acceptance

    Directory of Open Access Journals (Sweden)

    Daniel Simões Couto

    2011-12-01

    Full Text Available Reverse osmosis has been used for the concentration of fruit juices with promising considering the quality of the obtained products. The objective of this study was to concentrate single strength pineapple juice by reverse osmosis. The concentration was carried out with polyamide composite membranes in a 0.65 m² plate and frame module at 60 bar transmembrane pressure at 20 °C. The permeate flux was 17 L.hm-2. The total soluble solid content of the juice increased from 11 to 31 °Brix corresponding to a Volumetric Concentration Factor (VCF of 2.9. The concentration of soluble solids, total solids, and total acidity increased proportionally to FCV. The concentrated juice and three commercial concentrated pineapple juices were evaluated regarding preference and purchase intention by 79 pineapple juice consumers. The concentrated juice by reverse osmosis was the preferred among consumers. It can be concluded that this process may be considered an alternative to the pre-concentration of fruit juices.

  11. Mapping the rainfall distribution for irrigation planning in dry season at pineapple plantation, Lampung Province, Indonesia (Study case at Great Giant Pineapple Co. Ltd.)

    Science.gov (United States)

    Cahyono, P.; Astuti, N. K.; Purwito; Rahmat, A.

    2018-03-01

    One of the problems caused by climate change is unpredictable of the dry season. Understanding when the dry season will start is very important to planning the irrigation schedule especially on large plantation. Data of rainfall for 30 years in Lampung, especially in Pineapple Plantation show that dry month occurs from June to October. If in two decadals (ten days period) rainfall less than 100 mm then it is predicted that next decadal will be dry season. Great Giant Pineapple Co. Ltd. has 32,000 hectares plantation area and located in three regencies at Lampung Province, Indonesia with varies rainfall between regions within a plantation. Therefore, monitoring the rainfall distribution by using ombrometer installed at 10 representative location points can be used to determine irrigation period at the beginning of dry season. Mapping method using the server program and data source is from 10 monitoring rainfall stations installed at the observed points. Preparation of rainfall distribution mapping is important to know the beginning of the dry season and thus planning the irrigation. The results show that 2nd decadal of April is indicated as the starting time of dry season, which is similar with Indonesian government for climate agency’s result.

  12. Testicular histology and blood testosterone levels of male rabbit after given concentrated diets containing calliandra leaf meal and pineapple peels

    Science.gov (United States)

    Setyawati, I.; Ermayanti, N. G. A. M.; Suarni, N. M. R.; Narayani, I.; Suaskara, I. B. M.

    2018-03-01

    Calliandra calothyrsus Meissn is one of a highly protein source of forage, however, it is not widely used for non ruminant feed because it contains antinutritional substances in the form of condensed tannin. Tannin can bind proteins, this tannin-protein complexes are difficult to be digested in the gastrointestinal tract, then will be defecated outside the body. To optimize the utilization of calliandra in the diet, pineapple peels were added as a source of protease (bromelain). Beside of waste utilization, it is expected that bromelain can degrade the tannin-protein complexes thereby reducing the negative effects of tannins. This research is a feeding experiment on male rabbit (Lepus sp), five weeks old. The diet formulation is prepared according to the standardized diet of the local rabbit. This experiment used a Completely Randomized Design (CRD) with four treatments i.e. control group which were only given commercial feed (R0), commercial feed contained 15% of Calliandra leaf meal and 30% of pineapple peel (R1), commercial feed contained 30% of Calliandra leaf meal and 30% of pineapple peel (R2), and commercial feed contained 45% of Calliandra and 30% of pineapple peel (R3). The treatment was done for three months. The results of this study on the reproduction of male rabbits showed that the increase of calliandra leaf meal level in a diet containing 30% of pineapple peels affected the testicular histology, and also decreased the diameter of seminiferous tubule and blood testosterone levels of male rabbits.

  13. Taboo in the Workplace: A Qualitative Study of Taiwanese Nurses' "Not-Eating Pineapple" in Clinical Practice.

    Science.gov (United States)

    Tsai, Shu-Ling; Tsai, Chang-Hsiung; Hsu, Yu-Chien; Hsieh, Mei-Hui; Kao, Hsia-Tzu; Hsu, Min-Tao

    There has been an increased emphasis on nurses' mental health and well-being in the workplace. Psychologists have established a correlative link between individual's beliefs on luck and mental health. The pineapple taboo has been observed among Taiwanese hospital nurses as a prevalent superstitious belief for bringing luck or warding off increased clinical workloads, but how and why the ritual persists in the hospital workplace remains unknown. This article aims to explore the latent meaning of observance of the taboo and how it is related to nurses' clinical practice and possibly affects their mental health at work. A qualitative research was designed in line with the hermeneutic phenomenological method. Through purposive sampling, 18 nurse participants were recruited for in-depth semistructured interviews. Resulting from the ensuing analysis, 3 modalities were identified as constituting the spectrum of observance of the taboo: (a) "strictly not eating pineapple"; (b) "not eating pineapple at work"; and (c) "eating pineapple without admitting to doing so." Each reflects the position of nurses revealed in relation to the pineapple taboo in clinical settings. Based on the subjective narratives of nurses, it may be understood as an active moral attempt at "being right" rather than a passive avoidance of bad luck in the taboo observation. The findings facilitate an appropriate understanding of the embedded meaning of nurses' workplace-related belief and its seminal function of empowerment for nurses in holistic nursing practice.

  14. Influence of modified atmosphere packaging on volatile compounds and physicochemical and antioxidant attributes of fresh-cut pineapple (Ananas comosus).

    Science.gov (United States)

    Montero-Calderón, Marta; Rojas-Graü, María Alejandra; Aguiló-Aguayo, Ingrid; Soliva-Fortuny, Robert; Martín-Belloso, Olga

    2010-04-28

    The effects of modified atmosphere packaging on volatile compound content and physicochemical and antioxidant attributes of Gold cultivar fresh-cut pineapples were assessed throughout storage at 5 degrees C. Fresh-cut pineapple pieces were packed under LO (low oxygen, 12% O(2), 1% CO(2)), AIR (20.9% O(2)) and HO (high oxygen, 38% O(2)) headspace atmospheres. Methyl butanoate, methyl 2-methylbutanoate, and methyl hexanoate were the most abundant volatiles regardless of the packaging atmosphere and days of storage; whereas most odor active volatiles were methyl and ethyl 2-methylbutanoate, 2,5-dimethyl-4-methoxy-3(2H)-furanone and ethyl hexanoate. Physicochemical attributes of pineapple did not significantly vary, whereas vitamin C content and total antioxidant capacity were lower for fresh-cut pineapple in HO (488 +/- 38 mg/100 mg(fw) and 54.4 +/- 5.7%, respectively) than for LO and AIR packages. Storage life of fresh-cut pineapple was limited to 14 days by volatile compounds losses and fermentation processes.

  15. A novel aspartic acid protease gene from pineapple fruit (Ananas comosus): cloning, characterization and relation to postharvest chilling stress resistance.

    Science.gov (United States)

    Raimbault, Astrid-Kim; Zuily-Fodil, Yasmine; Soler, Alain; Cruz de Carvalho, Maria H

    2013-11-15

    A full-length cDNA encoding a putative aspartic acid protease (AcAP1) was isolated for the first time from the flesh of pineapple (Ananas comosus) fruit. The deduced sequence of AcAP1 showed all the common features of a typical plant aspartic protease phytepsin precursor. Analysis of AcAP1 gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development revealed opposite trends. The resistant variety showed an up-regulation of AcAP1 precursor gene expression whereas the susceptible showed a down-regulation in response to postharvest chilling treatment. The same trend was observed regarding specific AP enzyme activity in both varieties. Taken together our results support the involvement of AcAP1 in postharvest chilling stress resistance in pineapple fruits. Copyright © 2013 Elsevier GmbH. All rights reserved.

  16. Biology and trapping of stable flies (Diptera: Muscidae) developing in pineapple residues (Ananas comosus) in Costa Rica.

    Science.gov (United States)

    Solórzano, José-Arturo; Gilles, Jeremie; Bravo, Oscar; Vargas, Cristina; Gomez-Bonilla, Yannery; Bingham, Georgina V; Taylor, David B

    2015-01-01

    Pineapple production in Costa Rica increased nearly 300-fold during the last 30 yr, and >40,000 hectares of land are currently dedicated to this crop. At the end of the pineapple cropping cycle, plants are chopped and residues incorporated into the soil in preparation for replanting. Associated with increased pineapple production has been a large increase in stable fly, Stomoxys calcitrans (L.), populations. Stable flies are attracted to, and oviposit in, the decomposing, chopped pineapple residues. In conjunction with chemical control of developing larvae, adult trapping is an important control strategy. In this study, four blue-black fabric traps, Nzi, Vavoua, Model H, and Ngu, were compared with a white sticky trap currently used for stable fly control in Costa Rica. Overall, the white sticky trap caught the highest number of stable flies, followed by the Nzi, Vavoua, Model H, and Ngu. Collections on the white sticky trap increased 16 d after residues were chopped; coinciding with the expected emergence of flies developing in the pineapple residues. During this same time period, collections in the blue-black fabric traps decreased. Sex ratio decreased from >7:1 (females:males) 3-7 d after chopping to 1:1 at 24-28 d. White sticky, Nzi and Vavoua traps collected similar numbers of colonizing flies 3-7 d after residues were chopped. However, white sticky traps collected more flies once emergence from the pineapple residues began. Although white sticky traps collected more flies than fabric traps, they remain labor intensive and environmentally unsound because of their disposable and nonbiodegradable nature. Published by Oxford University Press on behalf of the Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.

  17. Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L.) Merr.] and papaya (Carica papaya L.)

    OpenAIRE

    Ramachandran Kogeethavani; Manaf Uyub Abdul; Zakaria Latiffah

    2015-01-01

    The Erwinia species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value fruit crops that include pineapple [Ananas comosus (L.) Merr.] and papaya (Carica papaya L.).The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species; Dickeya zeae from pineapple heart rot, and Erwinia mallotivora from papaya dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered...

  18. Trade-Offs of Flowering and Maturity Synchronisation for Pineapple Quality.

    Directory of Open Access Journals (Sweden)

    V Nicodème Fassinou Hotegni

    Full Text Available In the pineapple sector of Benin, poor fruit quality prevents pineapple producers to enter the European market. We investigated effects of common cultural practices, flowering and maturity synchronisation, (1 to quantify the trade-offs of flowering and maturity synchronisation for pineapple quality and the proportion of fruits exportable to European markets, and (2 to determine the effect of harvesting practice on quality attributes. Four on-farm experiments were conducted during three years using cultivars Sugarloaf and Smooth Cayenne. A split-split plot design was used in each experiment, with flowering induction practice as main factor (artificial or natural flowering induction, maturity induction practice as split factor (artificial or natural maturity induction and harvesting practice as the split-split factor (farmers' harvest practice or individual fruit harvesting at optimum maturity. Artificial flowering induction gave fruits with lower infructescence weight, higher ratio crown: infructescence length, and a lower proportion of fruits exportable to European markets than natural flowering induction. The costs of the improvements by natural flowering induction were huge: the longer durations from planting to flowering induction and harvesting, the higher number of harvestings of the fruits increasing the labour cost and the lower proportion of plants producing fruits compared with crops from artificially flowering-induced plants. Artificial maturity induction decreased the total soluble solids concentration in the fruits compared with natural maturity induction thus decreasing the proportion of fruits exportable to European markets, at a benefit of only a slightly shorter time from flowering induction to harvesting. Harvesting individual fruits at optimum maturity gave fruits with higher total soluble solids in naturally maturity induced fruits compared with the farmers' harvest practice. Given the huge costs of natural flowering induction

  19. Shelf life of minimally processed pineapples treated with ascorbic and citric acids

    Directory of Open Access Journals (Sweden)

    Lucimara Rogéria Antoniolli

    2012-01-01

    Full Text Available The purpose of this research was to determine the shelf life of minimally processed (MP 'Pérola' pineapples treated with ascorbic acid (AA and citric acid (CA based on physical, chemical, sensorial and microbiological attributes. Slices were dipped into drinking water (control or combined solutions of AA:CA (% (1.0:0.5 and 1.0:1.0 with sodium hypochlorite (NaClO 20 mg L-1 for 30 seconds. The samples were conditioned in polyethylene terephtalate packages and stored at 4±1 °C per 13 days. The low peroxidase activity in the slices treated with antioxidant combinations was related to low pH values observed in these samples. The treatments 1.0:0.5 and 1.0:1.0 (AA:CA, % favored maintenance of the initial a* values and avoided the pulp browning. The ascorbic acid increased more than double on the 2nd day in the treated slices. By the 4th day the CO2 values suggested a higher respiratory activity in the slices treated with anti-browning compounds. The antioxidant treatments did not produce detectable residual flavors in the MP pineapple. Regardless of microbiological safety during the 13 days of cold storage, the control slices can be kept by 6 days, afterwards the color and dehydration become strong enough to affect the appearance. On the other hand, MP 'Pérola' pineapples treated with 1.0:0.5 (AA:CA, % and NaClO (20 mg L-1 can be stored for 8 days at 4±1 ºC, which represents the extension of the shelf life in 2 days. After this period the overripe odor starts to develop.

  20. Development of Guided Inquiry-Based Student Lab Worksheet on the Making of Pineapple Flavoring

    Science.gov (United States)

    Dwiyanti, G.; Suryatna, A.; Taibah, I.

    2017-02-01

    The aim of this research was to develop guided inquiry based student lab worksheet on making pineapple flavour and knowing the quality of worksheet that is being developed. Research methods that is being conducted is research and development that is limited by a preliminary studies (literature studies, field surveys, and preparation of the initial product) and development of the model (within limited testing). The results from analyze the books sources and fields survey showed that the characteristic of esterification lab worksheet that currently available still in the direct instruction form (cookbook). The optimization result of making pineapple flavour experiment that was conducted are the ethanol volume 3 mL, butyric acid volume 2 mL, sulfuric acid 5 drops, saturated NaHCO3 solution volume 9 mL, and temperature of heating was 80 °C. The characteristic of guided inquiry based student lab worksheet that was developed contained phenomenon and instructions that suitable with inquiry stages to guide the students in doing the experiment of making pineapple flavour. The evaluation of designated teachers and lecturers of the developed student worksheet were very good (96,08%). Lab-experiment feasibility achieved by using guided inquiry based student lab worksheets that is being developed based on the inquiry stages that conducted by student were found very good (97,50%) and accomplishment based on students’ answer of the tasks in the worksheet were found very good (83,84%). Students’ responses of the experiments using the developed worksheet are found very good (81,84%).

  1. In vitro activity of pineapple extracts (Ananas comosus, Bromeliaceae) on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).

    Science.gov (United States)

    Domingues, Luciana Ferreira; Giglioti, Rodrigo; Feitosa, Karina Alves; Fantatto, Rafaela Regina; Rabelo, Márcio Dias; Oliveira, Márcia Cristina de Sena; Oliveira, Gilson Pereira de; Bechara, Gervasio Henrique; Chagas, Ana Carolina de Souza

    2013-07-01

    Measures to control the cattle tick, Rhipicephalus (Boophilus) microplus, based only on chemical products are becoming unsustainable, mainly because of the development of resistance. The objective of this study was to test the effect of the aqueous extract of pineapple skin (AEPS) and bromelain extracted from the stem (Sigma-Aldrich®, B4882) on engorged females and larvae of R. (B.) microplus in vitro. These substances were diluted in water and evaluated at eight concentrations. Engorged females were collected and distributed in groups of 10, with three repetitions for each treatment. After immersion in the solutions, the females were placed in an incubator for observation of survival, oviposition and larval hatching. The larval packet method was used, also with three repetitions with about 100 larvae each. The packets were incubated and the readings were performed after 24 h. The estimated reproduction and efficacy of the solutions were calculated. The LC(50) and LC(90) were estimated using the Probit procedure of the SAS program. The eight concentrations were compared within each treatment by the Tukey test. For the experiment with engorged females, the most effective concentrations were 125, 250 and 500 mg/mL: 33%, 48% and 59% for the AEPS and 27%, 51% and 55% for the bromelain. The LC(50) and LC(90) values were, respectively, 276 and 8691 mg/mL for AEPS and 373 and 5172 mg/mL for bromelain. None of the dilutions tested was effective against the larvae of R. (B.) microplus. This is the first report of the action of pineapple extracts or their constituents on cattle ticks. The results demonstrate that further studies regarding composition of tick cuticle, with evaluation of other solvents and formulations, should be conducted seeking to enhance the effect of pineapple extracts and compounds against this ectoparasite. Copyright © 2013 Elsevier Inc. All rights reserved.

  2. Soluble Sugars as the Carbohydrate Reserve for CAM in Pineapple Leaves 1

    Science.gov (United States)

    Carnal, Nancy Wieland; Black, Clanton C.

    1989-01-01

    Neutral ethanol-soluble sugar pools serve as carbohydrate reserves for Crassulacean acid metabolism (CAM) in pineapple (Ananas comosus (L.) Merr.) leaves. Levels of neutral soluble sugars and glucans fluctuated reciprocally with concentrations of malic acid. Hexose loss from neutral soluble-sugar pools was sufficient to account for malic acid accumulation with about 95% of the required hexose accounted for by turnover of fructose and glucose pools. Hexose loss from starch or starch plus lower molecular weight glucan pools was insufficient to account for nocturnal accumulation of malic acid. The apparent maximum catalytic capacity of pyrophosphate:6-phosphofructokinase (PPi-PFK) at 15°C was about 16 times higher than the mean maximum rate of glycolysis that occurred to support malic acid accumulation in pineapple leaves at night and 12 times higher than the mean maximum rate of hexose turnover from all carbohydrate pools. The apparent maximum catalytic capacity of ATP-PFK at 15°C was about 70% of the activity required to account for the mean maximal rate of hexose turnover from all carbohydrate pools if turnover were completely via glycolysis, and marginally sufficient to account for mean maximal rates of acidification. Therefore, at low night temperatures conducive to CAM and under subsaturating substrate concentrations, PPi-PFK activity, but not ATP-PFK activity, would be sufficient to support the rate of glycolytic carbohydrate processing required for acid accumulation. These data for pineapple establish that there are at least two types of CAM plants with respect to the nature of the carbohydrate reserve utilized to support nighttime CO2 accumulation. The data further indicate that the glycolytic carbohydrate processing that supports acidification proceeds in different subcellular compartments in plants utilizing different carbohydrate reserves. PMID:16666775

  3. Effect of chemical treatment on thermal properties of fibers from pineapple

    International Nuclear Information System (INIS)

    Fernandes, Rafael I.M.; Mulinari, Daniella R.; Carvalho, Kelly C.C.; Conejo, Luiza dos Santos; Voorwald, Herman J.C.; Cioffi, Maria Odila H.

    2009-01-01

    In this work the effect of the chemical modification of natural fibres from of pineapple fibres with alkaline solution was studied. After modification the in nature and modified fibres were analyzed by XRD diffractometry and thermogravimetry with objective to evaluate influence chemical treatment in surface and in the thermal properties fibres. With the obtained results it was possible to verify an increase of 10.4 % in the crystallinity index of fibres beyond the increase around 4.5% in the degradation temperature, what it indicates an increase in the stability thermal of the fibres. (author)

  4. Comparative study of two methods of fractionation bromelain from pineapple core extract (Ananas comosus)

    Science.gov (United States)

    Febriani, K.; Wahyuni, I.; Setiasih, S.; Hudiyono, S.

    2017-07-01

    The enzyme can be purified by fractional precipitation. This can be done by salt or organic solvent. In this research, purification of bromelain from pineapple core by fractional precipitation was done by 2 compounds, ammonium sulfate, and ethanol. Fractional precipitation by ammonium sulfate proved to be more effective as it yielded a higher specific activity. Specific activity by ethanol and ammonium sulfate is 4.6480 U/mg at 0-60 % saturation and 8.2243 U/mg at 50-80 % saturation.

  5. Ultrasound treatment on phenolic metabolism and antioxidant capacity of fresh-cut pineapple during cold storage.

    Science.gov (United States)

    Yeoh, Wei Keat; Ali, Asgar

    2017-02-01

    Ultrasound treatment at different power output (0, 25 and 29W) and exposure time (10 and 15min) was used to investigate its effect on the phenolic metabolism enzymes, total phenolic content and antioxidant capacity of fresh-cut pineapple. Following ultrasound treatment at 25 and 29W, the activity of phenylalanine ammonia lyase (PAL) was increased significantly (Ppineapple was significantly (Ppineapple. Results suggest that hormetic dosage of ultrasound treatment can enhance the activity of PAL and total phenolic content and hence the total antioxidant capacity to encounter with oxidative stress. Copyright © 2016. Published by Elsevier Ltd.

  6. Genome-wide organization and expression profiling of the R2R3-MYB transcription factor family in pineapple (Ananas comosus).

    Science.gov (United States)

    Liu, Chaoyang; Xie, Tao; Chen, Chenjie; Luan, Aiping; Long, Jianmei; Li, Chuhao; Ding, Yaqi; He, Yehua

    2017-07-01

    The MYB proteins comprise one of the largest families of plant transcription factors, which are involved in various plant physiological and biochemical processes. Pineapple (Ananas comosus) is one of three most important tropical fruits worldwide. The completion of pineapple genome sequencing provides a great opportunity to investigate the organization and evolutionary traits of pineapple MYB genes at the genome-wide level. In the present study, a total of 94 pineapple R2R3-MYB genes were identified and further phylogenetically classified into 26 subfamilies, as supported by the conserved gene structures and motif composition. Collinearity analysis indicated that the segmental duplication events played a crucial role in the expansion of pineapple MYB gene family. Further comparative phylogenetic analysis suggested that there have been functional divergences of MYB gene family during plant evolution. RNA-seq data from different tissues and developmental stages revealed distinct temporal and spatial expression profiles of the AcMYB genes. Further quantitative expression analysis showed the specific expression patterns of the selected putative stress-related AcMYB genes in response to distinct abiotic stress and hormonal treatments. The comprehensive expression analysis of the pineapple MYB genes, especially the tissue-preferential and stress-responsive genes, could provide valuable clues for further function characterization. In this work, we systematically identified AcMYB genes by analyzing the pineapple genome sequence using a set of bioinformatics approaches. Our findings provide a global insight into the organization, phylogeny and expression patterns of the pineapple R2R3-MYB genes, and hence contribute to the greater understanding of their biological roles in pineapple.

  7. Ripening-dependent metabolic changes in the volatiles of pineapple (Ananas comosus (L.) Merr.) fruit: II. Multivariate statistical profiling of pineapple aroma compounds based on comprehensive two-dimensional gas chromatography-mass spectrometry.

    Science.gov (United States)

    Steingass, Christof Björn; Jutzi, Manfred; Müller, Jenny; Carle, Reinhold; Schmarr, Hans-Georg

    2015-03-01

    Ripening-dependent changes of pineapple volatiles were studied in a nontargeted profiling analysis. Volatiles were isolated via headspace solid phase microextraction and analyzed by comprehensive 2D gas chromatography and mass spectrometry (HS-SPME-GC×GC-qMS). Profile patterns presented in the contour plots were evaluated applying image processing techniques and subsequent multivariate statistical data analysis. Statistical methods comprised unsupervised hierarchical cluster analysis (HCA) and principal component analysis (PCA) to classify the samples. Supervised partial least squares discriminant analysis (PLS-DA) and partial least squares (PLS) regression were applied to discriminate different ripening stages and describe the development of volatiles during postharvest storage, respectively. Hereby, substantial chemical markers allowing for class separation were revealed. The workflow permitted the rapid distinction between premature green-ripe pineapples and postharvest-ripened sea-freighted fruits. Volatile profiles of fully ripe air-freighted pineapples were similar to those of green-ripe fruits postharvest ripened for 6 days after simulated sea freight export, after PCA with only two principal components. However, PCA considering also the third principal component allowed differentiation between air-freighted fruits and the four progressing postharvest maturity stages of sea-freighted pineapples.

  8. 77 FR 24459 - Voluntary Termination of Foreign-Trade Subzone 9D, Maui Pineapple Company, Ltd., Kahului, Maui, HI

    Science.gov (United States)

    2012-04-24

    ... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Order No. 1821] Voluntary Termination of Foreign-Trade Subzone 9D, Maui Pineapple Company, Ltd., Kahului, Maui, HI Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934, as amended (19 U.S.C. 81a-81u), and the Foreign-Trade Zones...

  9. Pollination of pima pineapple cactus (Coryphantha sheeri var. robustispina): does pollen flow limit abundance of this endangered species?

    Science.gov (United States)

    Christopher J. McDonald; Guy R. McPherson

    2005-01-01

    Pima pineapple cactus (PPC) (Coryphantha sheeri var. robustispina), a federally listed endangered species, occurs throughout southeastern Arizona and has relatively low population densities. To determine whether pollination limits reproduction of PPC we used florescent dye to quantify pollen flow between individuals in a PPC...

  10. Effect of membrane property and operating conditions on phytochemical properties and permeate flux during clarification of pineapple juice

    KAUST Repository

    Laorko, Aporn; Li, Zhenyu; Tongchitpakdee, Sasitorn; Chantachum, Suphitchaya; Youravong, Wirote

    2010-01-01

    The effects of membrane property on the permeate flux, membrane fouling and quality of clarified pineapple juice were studied. Both microfiltration (membrane pore size of 0.1 and 0.2 μm) and ultrafiltration (membrane molecular weight cut-off (MWCO) of 30 and 100 kDa) membranes were employed. Membrane filtration did not have significant effects on the pH, reducing sugar and acidity of clarified juice whereas the suspended solids and microorganism were completely removed. The 0.2 μm membrane gave the highest permeate flux, total vitamin C content, total phenolic content and antioxidant capacity as well as the highest value of irreversible fouling. Based on these results, the membrane with pore size of 0.2 μm was considered to be the most suitable membrane for the clarification of pineapple juice. The optimum operating conditions for the clarification pineapple juice by membrane filtration was a cross-flow velocity of 3.4 ms-1 and transmembrane pressure (TMP) of 0.7 bar. An average flux of about 37 lm-2 h-1 was obtained during the microfiltration of pineapple juice under the optimum conditions using batch concentration mode. © 2010 Elsevier Ltd. All rights reserved.

  11. Effect of membrane property and operating conditions on phytochemical properties and permeate flux during clarification of pineapple juice

    KAUST Repository

    Laorko, Aporn

    2010-10-01

    The effects of membrane property on the permeate flux, membrane fouling and quality of clarified pineapple juice were studied. Both microfiltration (membrane pore size of 0.1 and 0.2 μm) and ultrafiltration (membrane molecular weight cut-off (MWCO) of 30 and 100 kDa) membranes were employed. Membrane filtration did not have significant effects on the pH, reducing sugar and acidity of clarified juice whereas the suspended solids and microorganism were completely removed. The 0.2 μm membrane gave the highest permeate flux, total vitamin C content, total phenolic content and antioxidant capacity as well as the highest value of irreversible fouling. Based on these results, the membrane with pore size of 0.2 μm was considered to be the most suitable membrane for the clarification of pineapple juice. The optimum operating conditions for the clarification pineapple juice by membrane filtration was a cross-flow velocity of 3.4 ms-1 and transmembrane pressure (TMP) of 0.7 bar. An average flux of about 37 lm-2 h-1 was obtained during the microfiltration of pineapple juice under the optimum conditions using batch concentration mode. © 2010 Elsevier Ltd. All rights reserved.

  12. Governance, marketing and innovations in Beninese pineapple supply chains : a survey of smallholder farmers in South Benin

    NARCIS (Netherlands)

    Arinloye, D.D.A.A.

    2013-01-01

    The main goal of the study was to identify an innovative approach that could overcome market quality and price information asymmetry issues in pineapple supply chain in Benin. Two case studies were conducted in Benin and Ghana, with an in-depth survey of 219 farmers in Benin. The study mapped

  13. Multi-governance choices by smallholder farmers in the pineapple supply chain in Benin: An application of transaction cost theory

    NARCIS (Netherlands)

    Arinloye, A.A.D.D.; Hagelaar, J.L.F.; Linnemann, A.R.; Pascucci, S.; Coulibaly, O.; Omta, S.W.F.; Boekel, van T.

    2012-01-01

    The present study validates the new construct “Multi-governance choices”, to sharpen our understanding of how and why smallholder farmers select among alternative governance structures. Primary data were collected from a sample of 219 pineapple farmers in Southern Benin. Results from a multivariate

  14. Electrodialytic removal of nitrate from pineapple juice: effect on selected physicochemical properties, amino acids, and aroma components of the juice.

    Science.gov (United States)

    Ackarabanpojoue, Yuwadee; Chindapan, Nathamol; Yoovidhya, Tipaporn; Devahastin, Sakamon

    2015-05-01

    This study aimed at investigating the effect of nitrate removal from pineapple juice by electrodialysis (ED) on selected properties of the ED-treated juice. Single-strength pineapple juice with reduced pulp content was treated by ED to reduce the nitrate concentration to 15, 10, or 5 ppm. After ED, the removed pulp was added to the ED-treated juice and its properties, including electrical conductivity, acidity, pH, total soluble solids (TSS), color, amino acids, and selected aroma compounds, were determined and compared with those of the untreated juice. ED could reduce the nitrate content of 1 L of pineapple juice from an initial value of 50 ppm to less than 5 ppm within 30 min. A significant decrease in the electrical conductivity, acidity, pH, TSS, and yellowness, but a significant increase in the lightness, of the juice was observed upon ED. Concentrations of almost all amino acids of the ED-treated juice significantly decreased. The concentrations of 8 major compound contributors to the pineapple aroma also significantly decreased. Adding the pulp back to the ED-treated juice increased the amino acids concentrations; however, it led to a significant decrease in the concentrations of the aroma compounds. © 2015 Institute of Food Technologists®

  15. Pineapple (Ananas comosus L. Merr.) wine production in Angola: Characterisation of volatile aroma compounds and yeast native flora.

    Science.gov (United States)

    Dellacassa, Eduardo; Trenchs, Oriol; Fariña, Laura; Debernardis, Florencia; Perez, Gabriel; Boido, Eduardo; Carrau, Francisco

    2017-01-16

    A pineapple vinification process was conducted through inoculated and spontaneous fermentation to develop a process suitable for a quality beverage during two successive vintages in Huambo, Angola. Wines obtained with the conventional Saccharomyces cerevisiae strain, were analysed by gas chromatography, and a total of 61 volatile constituents were detected in the volatile fraction and 18 as glycosidically bound aroma compounds. Concentration levels of carbonyl and sulphur compounds were in agreement with the limited information reported about pineapple fruits of other regions. We report, for the first time in pineapple wines, the presence of significant concentrations of lactones, ketones, terpenes, norisoprenoids and a variety of volatile phenols. Eight native yeast strains were isolated from spontaneous batches. Further single-strain fermentations allowed us to characterise their suitability for commercial fermentation. Three native strains (Hanseniaspora opuntiae, H. uvarum and Meyerozyma guilliermondii) were selected with sensory potential to ferment pineapple fruits with increased flavour diversity. Results obtained here contribute to a better understanding of quality fermentation alternatives of this tropical fruit in subtropical regions. Copyright © 2016 Elsevier B.V. All rights reserved.

  16. Current status of tropical fruit breeding and genetics for three tropical fruit species cultivated in Japan: pineapple, mango, and papaya

    Science.gov (United States)

    Ogata, Tatsushi; Yamanaka, Shinsuke; Shoda, Moriyuki; Urasaki, Naoya; Yamamoto, Toshiya

    2016-01-01

    Tropical fruit crops are predominantly produced in tropical and subtropical developing countries, but some are now grown in southern Japan. Pineapple (Ananas comosus), mango (Mangifera indica) and papaya (Carica papaya) are major tropical fruits cultivated in Japan. Modern, well-organized breeding systems have not yet been developed for most tropical fruit species. Most parts of Japan are in the temperate climate zone, but some southern areas such as the Ryukyu Islands, which stretch from Kyushu to Taiwan, are at the northern limits for tropical fruit production without artificial heating. In this review, we describe the current status of tropical fruit breeding, genetics, genomics, and biotechnology of three main tropical fruits (pineapple, mango, and papaya) that are cultivated and consumed in Japan. More than ten new elite cultivars of pineapple have been released with improved fruit quality and suitability for consumption as fresh fruit. New challenges and perspectives for obtaining high fruit quality are discussed in the context of breeding programs for pineapple. PMID:27069392

  17. Quality optimisation of combined osmotic dehydration and microwave assisted air drying of pineapple using constant power emission

    CSIR Research Space (South Africa)

    Botha, GE

    2012-04-01

    Full Text Available parameters that influenced the quality of the dehydrated pineapple, with the parameters most affected by the operating conditions being water content and percentage of charred pieces. Only in the latter was a significant quadratic effect found, all others...

  18. Effect of starch sizes particle as binder on short pineapple leaf fiber composite mechanical properties

    Directory of Open Access Journals (Sweden)

    Selamat Mohd Zulkefli

    2018-01-01

    Full Text Available Pineapple leaf fiber (PLF is one of the natural fibers that abundantly can be found in Malaysia, but the usage of the pineapple plant is limited only on their fruit and the other parts to be a waste. In this study, PLF is used as the reinforcement material and starch (SH used as the matrix or binder. Both materials were combined with several compositions ratio (weight percentage of PLF/SH composites which are 50PLF/50SH, 60PLF/40SH and 70PLF/30SH. Before undergo the fabrication process, the fiber has gone through an alkaline treatment to increase the strength of the fiber and chopped with an approximate size range from 0.5 mm to 5 mm. Besides that, SH powder is sieved to gain several particulate sizes which are 75 μm, 100 μm and 250 μm. The related tests such as flexural, hardness, density tests and macrostructure analysis have been done to determine their mechanical properties of composite. Based on the results, the sample with composition of 70PL/30SH with 75 μm has shown the highest result for flexural stress which is 14.49 MPa. While, the composite with the same composition of 70PLF/30SH with particulate size SH of 250 μm has shown the highest result in the hardness of 67 Shore-D and density of 1.36 g/cm3 respectively.

  19. Growth model of the pineapple guava fruit as a function of thermal time and altitude

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    Alfonso Parra-Coronado

    2016-09-01

    Full Text Available The growth of the pineapple guava fruit is primarily stimulated by temperature but is also influenced by other climactic factors, such as altitude. The goal of this study was to develop a growth model for the pineapple guava fruit as a function of thermal time (GDD, growing-degree day and altitude (H of the production area. Twenty trees per farm were marked in two sites in the Cundinamarca department (Colombia during the 2012 and 2014 seasons. The measurements were performed every seven days after day 96 and 99 post-anthesis until harvest in the sites of Tenjo (2,580 m.a.s.l. and San Francisco de Sales (1,800 m.a.s.l., respectively. A growth model was produced for weight as a function of fruit length and diameter as well as for the weight of the fruit as a function of GDD and H, with this last measure adjusted to a sigmoidal logistic growth model. The parameters for the regression analysis showed that the models satisfactorily predicted fruit growth for both of the sites, with a high determination coefficient. The cross-validation showed good statistical fit between the predicted and observed models; the intercept was not significantly different than zero, and the slope was statistically equal to one.

  20. Development and quality of pineapple guava fruit in two locations with different altitudes in Cundinamarca, Colombia

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    Alfonso Parra-Coronado

    2015-01-01

    Full Text Available Fruit growth is stimulated by different weather conditions. The aim of this study was to determine the influence of weather conditions on the physicochemical properties of pineapple guava fruit growth. Twenty trees were marked in two production areas located at different altitudes (1,800 and 2,580 m.a.s.l., and measurements were performed every 7 days from 99 and 141 days post-anthesis to harvest at altitudes of 1,800 and 2,580 m.a.s.l., respectively. The results indicate that altitude and weather conditions greatly influence the growth and development of pineapple guava fruit, and these effects are primarily manifested in the physical characteristics of the fruit. The weight and size of the fruit at harvest are directly related to the altitude of the production area. The weather condition that has the greatest impact on total titratable acidity at harvest is cumulative radiation during fruit growth; the highest value of total soluble solids at harvest corresponds to the location with the higher altitude, lower rainfall and relative humidity and higher cumulative radiation during the fruit growth period. The hue angle and pulp firmness at harvest are not influenced by the location or weather conditions at any location and do not determine the fruit quality at harvest time.

  1. Design of automatic control system for the precipitation of bromelain from the extract of pineapple wastes

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    Flavio Vasconcelos da Silva

    2010-12-01

    Full Text Available In this work, bromelain was recovered from ground pineapple stem and rind by means of precipitation with alcohol at low temperature. Bromelain is the name of a group of powerful protein-digesting, or proteolytic, enzymes that are particularly useful for reducing muscle and tissue inflammation and as a digestive aid. Temperature control is crucial to avoid irreversible protein denaturation and consequently to improve the quality of the enzyme recovered. The process was carried out alternatively in two fed-batch pilot tanks: a glass tank and a stainless steel tank. Aliquots containing 100 mL of pineapple aqueous extract were fed into the tank. Inside the jacketed tank, the protein was exposed to unsteady operating conditions during the addition of the precipitating agent (ethanol 99.5% because the dilution ratio "aqueous extract to ethanol" and heat transfer area changed. The coolant flow rate was manipulated through a variable speed pump. Fine tuned conventional and adaptive PID controllers were on-line implemented using a fieldbus digital control system. The processing performance efficiency was enhanced and so was the quality (enzyme activity of the product.

  2. Screening of Acetic Acid Bacteria from Pineapple Waste for Bacterial Cellulose Production using Sago Liquid Waste

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    Nur Arfa Yanti

    2017-12-01

    Full Text Available Bacterial cellulose is a biopolymer produced by fermentation process with the help of bacteria. It has numerous applications in industrial sector with its characteristic as a biodegradable and nontoxic compound in nature. The potential application of BC is limited by its production costs, because BC is produced from expensive culture media. The use of cheap carbon and nutrient sources such as sago liquid waste is an interesting strategy to overcome this limitation. The objective of this study was to obtain the AAB strain that capable to produce bacterial cellulose from sago liquid waste. Isolation of AAB strains was conducted using CARR media and the screening of BC production was performed on Hestrin-Schramm (HS media with glucose as a carbon source. The strains of AAB then were evaluated for their cellulose-producing capability using sago liquid waste as a substrate. Thirteen strains of AAB producing BC were isolated from pineapple waste (pineapple core and peel and seven of them were capable to produce BC using sago liquid waste substrate. One of the AAB strains produced a relatively high BC, i.e. isolate LKN6. The result of morphological and biochemical test was proven that the bacteria was Acetobacter xylinum. The result of this study showed that A. xylinum LKN6 can produce a high yield of BC, therefore this strain is potentially useful for its utilization as a starter in bacterial cellulose production. 

  3. Diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple plantlets during acclimatization

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    Aurilena de Aviz Silva

    Full Text Available ABSTRACT: This study examines the effect of inoculation with diazotrophic bacteria and nitrogen fertilization on the growth of micropropagated pineapple cv. 'Vitória' plantlets during the acclimatization period. The experiment was carried out in a greenhouse in Campos dos Goytacazes, in randomized blocks, using a 2x5x5 factorial scheme, with the factors being two types of inocula (absence or presence of a mixture of diazotrophic bacteria that contained Burkholderia sp. UENF 114111, Burkholderia silvatlantica UENF 11711, and Herbaspirillum seropedicae HRC 54, five levels of urea (0, 2, 5, 7, and 10g L-1, and five evaluation periods (30, 60, 90, 120, and 150 days following the planting of plantlets in trays, and with four replications of each treatment. An increase in the dried weight of shoots and roots of micropropagated pineapple cv. 'Vitória' plants depended on inoculation with diazotrophic bacteria, and plantlets acclimatization could be reduced by up to 57 days, depending on the concentration of urea fertilizer.

  4. Effect of Extraction Process and Surface Treatment on the mechanical properties in Pineapple Leaf Fibre

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    Ariffin Azrie

    2017-01-01

    Full Text Available Pineapple Leaf Fibre (PALF is a one of the natural fibre that has high potential in the industry. Natural fibres have become the main alternative source in the modern world industry. The objective of this study is to observe the effect chemical treatment using Sodium Hydroxide (NaOH solution on the physical and mechanical properties of pineapple leaf fibre. Different concentration of NaOH solution (2%, 4%, 6%, 8% and different treatment time (1 hour, 3 hour and 5 hour are used for the experiment. The tensile test was conducted to obtain the mechanical properties such as tensile strength, Yong modulus, (E and elongation at break. From the results obtained, NaOH concentration of 6% and five-hour treatment time that was used for treatment showed the higher physical and mechanical properties values. Furthermore, morphology analysis also shows the surface of the fibre at 6% NaOH after five-hour of treatment was in the better condition with good bonding arrangement of the fibre.

  5. Novel biocomposite of carboxymethyl chitosan and pineapple peel carboxymethylcellulose as sunscreen carrier.

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    Wongkom, Lucksanee; Jimtaisong, Ampa

    2017-02-01

    This study aims to prepare of biocomposite of carboxymethyl chitosan (CM-chitosan) and carboxymethylcellulose (CMC) from Ananas comosus (pineapple) peel for use as broad spectrum sunscreen carrier. Biocomposite was produced by using ferulic acid (FA), a plant extract, as crosslinker with the optimal ratio of CMC: CM-chitosan: FA at 1:2:4%w. FT-IR technique demonstrated that crosslinking may occur at amine group of CM-chitosan and carboxyl group of FA and hydrogen bonding between hydroxyl group of CMC and carboxyl group of FA. Biocomposite is pale yellow powder and present fibre bundle-like surface in the SEM image. DSC, TGA and XRD results indicated that new compound was formed. The particle size of biocomposite is 626nm determined by using Zetasizer. Hydrophilic TiO 2 and phenylbenzimidazole sulphonic acid (PBSA) were used as sunscreen agent at ratio of TiO 2 : PBSA at 2:1%w. The biocomposite sunscreen possesses the SPF value of 2.47 with boost star rating of 3 at 2% compound. The results obtained indicate that the biocomposite was successfully prepared from CM-chitosan and pineapple peel CMC and the system can be used as matrix delivery system for hydrophilic sunscreens. Copyright © 2016 Elsevier B.V. All rights reserved.

  6. Adsorption of caffeine on mesoporous activated carbon fibers prepared from pineapple plant leaves.

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    Beltrame, Karla K; Cazetta, André L; de Souza, Patrícia S C; Spessato, Lucas; Silva, Taís L; Almeida, Vitor C

    2018-01-01

    The present work reports the preparation of activated carbon fibers (ACFs) from pineapple plant leaves, and its application on caffeine (CFN) removal from aqueous solution. The preparation procedure was carried out using the H 3 PO 4 as activating agent and slow pyrolysis under N 2 atmosphere. The characterization of materials was performed from the N 2 adsorption and desorption isotherms, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Raman spectroscopy, Boehm titration and pH pzc method. ACFs showed high BET surface area value (S BET = 1031m 2 g -1 ), well-developed mesoporous structure (mesopore volume of 1.27cm³ g -1 ) and pores with average diameter (D M ) of 5.87nm. Additionally, ACFs showed features of fibrous material with predominance of acid groups on its surface. Adsorption studies indicated that the pseudo-second order kinetic and Langmuir isotherm models were that best fitted to the experimental data. The monolayer adsorption capacity was found to be 155.50mgg -1 . thermodynamic studies revealed that adsorption process is spontaneous, exothermic and occurs preferably via physisorption. The pineapple leaves are an efficient precursor for preparation of ACFs, which were successful applied as adsorbent material for removal of caffeine from the aqueous solutions. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. Plasticity of crassulacean acid metabolism at subtropical latitudes: a pineapple case study.

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    Rainha, Nuno; Medeiros, Violante P; Câmara, Mariana; Faustino, Hélder; Leite, João P; Barreto, Maria do Carmo; Cruz, Cristina; Pacheco, Carlos A; Ponte, Duarte; Bernardes da Silva, Anabela

    2016-01-01

    Plants with the crassulacean acid metabolism (CAM) express high-metabolic plasticity, to adjust to environmental stresses. This article hypothesizes that irradiance and nocturnal temperatures are the major limitations for CAM at higher latitudes such as the Azores (37°45'N). Circadian CAM expression in Ananas comosus L. Merr. (pineapple) was assessed by the diurnal pattern of leaf carbon fixation into l-malate at the solstices and equinoxes, and confirmed by determining maximal phosphoenolpyruvate carboxylase (PEPC) activity in plant material. Metabolic adjustments to environmental conditions were confirmed by gas exchange measurements, and integrated with environmental data to determine CAM's limiting factors: light and temperature. CAM plasticity was observed at the equinoxes, under similar photoperiods, but different environmental conditions. In spring, CAM expression was similar between vegetative and flowering plants, while in autumn, flowering (before anthesis) and fructifying (with fully developed fruit before ripening) plants accumulated more l-malate. Below 100 µmol m(-2) s(-1) , CAM phase I was extended, reducing CAM phase III during the day. Carbon fixation inhibition may occur by two major pathways: nocturnal temperature (pineapple to survive in environments not suitable for high productivity. © 2015 Scandinavian Plant Physiology Society.

  8. Temporal and spatial transcriptomic and microRNA dynamics of CAM photosynthesis in pineapple.

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    Wai, Ching M; VanBuren, Robert; Zhang, Jisen; Huang, Lixian; Miao, Wenjing; Edger, Patrick P; Yim, Won C; Priest, Henry D; Meyers, Blake C; Mockler, Todd; Smith, J Andrew C; Cushman, John C; Ming, Ray

    2017-10-01

    The altered carbon assimilation pathway of crassulacean acid metabolism (CAM) photosynthesis results in an up to 80% higher water-use efficiency than C 3 photosynthesis in plants making it a potentially useful pathway for engineering crop plants with improved drought tolerance. Here we surveyed detailed temporal (diel time course) and spatial (across a leaf gradient) gene and microRNA (miRNA) expression patterns in the obligate CAM plant pineapple [Ananas comosus (L.) Merr.]. The high-resolution transcriptome atlas allowed us to distinguish between CAM-related and non-CAM gene copies. A differential gene co-expression network across green and white leaf diel datasets identified genes with circadian oscillation, CAM-related functions, and source-sink relations. Gene co-expression clusters containing CAM pathway genes are enriched with clock-associated cis-elements, suggesting circadian regulation of CAM. About 20% of pineapple microRNAs have diel expression patterns, with several that target key CAM-related genes. Expression and physiology data provide a model for CAM-specific carbohydrate flux and long-distance hexose transport. Together these resources provide a list of candidate genes for targeted engineering of CAM into C 3 photosynthesis crop species. © 2017 The Authors The Plant Journal © 2017 John Wiley & Sons Ltd.

  9. Cell wall alterations in the leaves of fusariosis-resistant and susceptible pineapple cultivars.

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    de Farias Viégas Aquije, Glória Maria; Zorzal, Poliana Belisário; Buss, David Shaun; Ventura, José Aires; Fernandes, Patricia Machado Bueno; Fernandes, Antonio Alberto Ribeiro

    2010-10-01

    Fusariosis, caused by the fungus Fusarium subglutinans f. sp. ananas (Syn. F. guttiforme), is one of the main phytosanitary threats to pineapple (Ananas comosus var. comosus). Identification of plant cell responses to pathogens is important in understanding the plant-pathogen relationship and establishing strategies to improve and select resistant cultivars. Studies of the structural properties and phenolic content of cell walls in resistant (Vitoria) and susceptible (Perola) pineapple cultivars, related to resistance to the fungus, were performed. The non-chlorophyll base of physiologically mature leaves was inoculated with a conidia suspension. Analyses were performed post-inoculation by light, atomic force, scanning and transmission electron microscopy, and measurement of cell wall-bound phenolic compounds. Non-inoculated leaves were used as controls to define the constitutive tissue characteristics. Analyses indicated that morphological differences, such as cell wall thickness, cicatrization process and lignification, were related to resistance to the pathogen. Atomic force microscopy indicated a considerable difference in the mechanical properties of the resistant and susceptible cultivars, with more structural integrity, associated with higher levels of cell wall-bound phenolics, found in the resistant cultivar. p-Coumaric and ferulic acids were shown to be the major phenolics bound to the cell walls and were found in higher amounts in the resistant cultivar. Leaves of the resistant cultivar had reduced fungal penetration and a faster and more effective cicatrization response compared to the susceptible cultivar.

  10. Macronutrients accumulation and growth of pineapple cultivars submitted to aluminum stress

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    Mauro F. C. Mota

    Full Text Available ABSTRACT The objective was to determine the growth and accumulation of macronutrients of two pineapple cultivars submitted to different concentrations of aluminum (Al. For this, a study was conducted in plastic pots containing 4 L of nutrient solution, in a randomized block design, in a 2 x 5 factorial scheme, corresponding to two pineapple cultivars (‘IAC Fantástico’ and ‘Vitória’ and five Al concentrations (0, 21.6, 43.2, 64.8 and 86.4 mg of Al plant-1, with four replicates. The following variables were evaluated: root length, dry matter of root, stem and leaf, stem diameter, number of leaves, chlorophyll content and accumulation of macronutrients at 60 days after treatment. The cv. ‘Vitória’ showed a linear decrease in chlorophyll content, root dry matter, root length and accumulation of N, P, K, Ca and Mg in most plant components promoted by the increase of Al concentration in the nutrient solution. The cv. ‘IAC Fantástico’ had lower total dry matter, stem dry matter, stem diameter and accumulation of N, Ca and Mg. However, the evaluated characteristics were not influenced by the increase of Al concentration, showing greater tolerance of this cultivar to Al in nutrient solution.

  11. Purification, characterization, and antifungal activity of chitinases from pineapple (Ananas comosus) leaf.

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    Taira, Toki; Toma, Noriko; Ishihara, Masanobu

    2005-01-01

    Three chitinases, designated pineapple leaf chitinase (PL Chi)-A, -B, and -C were purified from the leaves of pineapple (Ananas comosus) using chitin affinity column chromatography followed by several column chromatographies. PL Chi-A is a class III chitinase having a molecular mass of 25 kDa and an isoelectric point of 4.4. PL Chi-B and -C are class I chitinases having molecular masses of 33 kDa and 39 kDa and isoelectric points of 7.9 and 4.6 respectively. PL Chi-C is a glycoprotein and the others are simple proteins. The optimum pHs of PL Chi-A, -B, and -C toward glycolchitin are pH 3, 4, and 9 respectively. The chitin-binding ability of PL Chi-C is higher than that of PL Chi-B, and PL Chi-A has lower chitin-binding ability than the others. At low ionic strength, PL Chi-B exhibits strong antifungal activity toward Trichoderma viride but the others do not. At high ionic strength, PL Chi-B and -C exhibit strong and weak antifungal activity respectively. PL Chi-A does not have antifungal activity.

  12. [Determination of 1, 3-O-dicaffeoylglycerides from leaves of pineapple by HPLC].

    Science.gov (United States)

    Shen, Jia; Xie, Wei-Dong; Wang, Wei; Ding, Yi; Du, Li-Jun

    2007-05-01

    To establish a HPLC method for a new compound 1,3-O-dicaffeoylglycerides determination. The separation was performed in a Kromasil C18 column (4.6 mm x 150 mm, 5 microm) with a mobile phase of 0.1% H3PO4: Acetontrile = 75 : 25 (v/v). The flow rate was 1.0 ml x min(-)1 and the temperature of column was 30 degrees C. A satisfactory separation between 1, 3-O-dicaffeoylglycerides and impurity was obtained. The calibration curve was linear over the concentration range from 0.051 6 microg to 0.516 microg, r = 0.999 9. The average recoveries was 97.1% (RSD 1.3%). The content of 1,3-O-dicaffeoylglycerides in pineapple leaves from three different batches were 0.033%, 0.034% and 0.031% respectively. The method has good selectivity, high recovery and reproducibility, and can be used for the analysis of 1,3-O-dicaffeoylglycerides in pineapple leaves and their quality control.

  13. In vitro regeneration of Amazonian pineapple (Ananas comosus plants ecotype Gobernadora

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    Héctor Alexander Blanco Flores

    2017-01-01

    Full Text Available There are a number of pineapple (Ananas comosus cultivars and ecotypes of local commercial importance in Venezuela, among them the Amazonian ones, cultivated mainly by the aboriginal Piaroa, are of relevance. They sow the propagules, which restricts the availability of material for large-scale cultivation. This limitation was approached by plant tissue culture for in vitro propagation of Amazonian pineapple plants, Gobernadora ecotype, through somatic embryogenesis (ES and adventitious organogenesis (OA. Basal and intermediate sections of leaves were tested. Only the leaf base sections (FBS were morphogenically induced. The highest number of vitroplants (1.58 plants / explant was obtained from the embryogenic callus induced in MS medium with Picloram 10 mg.L-1 + Thidiazuron 2 mg.L-1, transferred to MS medium without hormones. In the organogenic process, the highest number of plants/explants (5 was obtained directly in MS with naphthaleneacetic acid 5 mg.L-1 + benzylaminopurine 0.25 mg.L-1, transferred to MS. The latter being the best in vitro culture system due to its productivity and for being a method that minimizes somaclonal variation.

  14. Analysis of the Perolera and Gold pineapple into two main wholesalers central Colombia

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    Ana María Neira García

    2016-05-01

    Full Text Available In this work the behavior of the price of two varieties of pineapple Gold and wholesalers Perolera two plants in Colombia is analyzed. The techniques used were the cointegration and Granger causality test, for which prices were taken pineapple, in the period 2006-2011 of wholesale markets Corabastos (in the case of Bogotá and the Central Wholesale Itagui (for Medellin. The theoretical framework was the law of one price. The work proved that there is market integration in the process of price formation. The results allow us to see that there is independence between the prices of the two markets. Cointegration tests indicate that the series are cointegrated itself, so that in the absence of external shocks, the series converge in the long term. We conclude that, although there is no causal link between the markets, these are themselves integrated in the long run, evidencing a fulfillment of the law of one price. The study does not include an analysis of the distances between production areas and consumption centers to consider the effect of transport costs when setting the price. Future research could consider such variables as quality and consumer preferences.

  15. Integrated system for recommending fertilization rates in pineapple (Ananas comosus (L. Merr. crop

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    Sergio Salgado García

    2017-10-01

    Full Text Available In recent years much attention has focused on the impacts of agriculture on climate change, due to this stage specialists in plant nutrition and soil fertility have achieved the task of generating adequate fertilization doses for pineapple to reduce effects on environment. A methodology for Integrated System for Recommending Fertilizer Dose (ISRFD was used. As a result, seven Thiessen polygons of the average annual rainfall, where rainfall ranged from 1640 to 2841 mm was correlated. Therefore, three major soil groups were defined and classified as subunit level. Likewise, eight doses of fertilizers were generated as follows: N, P2O5 and K2O, with a fertilizer dose, a map is generated according to the cultivar: 230-138-300 for Creole pineapple in Acrisol Cutanic (Chromic, Ferric; 460-161-480 for Cayenne and MD2 in Acrisol Cutanic (Endoclayic, Ferric; 345-161-450 for Cayenne and MD2, 253-138-450 for Creole in Acrisol Cutanic (Endoclayic, Hyperdystric, Ferric; 391-161-450 for Cayenne and MD2 in Acrisol Umbric Cutanic (Endoclayic, Hyperdystric and Acrisol Umbric Cutanic (Endoclayic, Hyperdystric, Ferric; 207-138-300 for Creole in Acrisol Umbric Cutanic (Endoclayic, Hyperdystric; 253-138-300 for Creole in Acrisol Umbric Cutanic (Endoclayic,Hyperdystric, Ferric; 253-138-360 for Creole in Acrisol Umbric Gleyic (Hyperdystric, Ferric; and 391-161-360 in Cambisol Endogleyic (Clayic, Eutric. These fertilizer doses were supplemented with micronutrients to obtain the expected results.

  16. Complete chloroplast genome sequence of MD-2 pineapple and its comparative analysis among nine other plants from the subclass Commelinidae.

    Science.gov (United States)

    Redwan, R M; Saidin, A; Kumar, S V

    2015-08-12

    Pineapple (Ananas comosus var. comosus) is known as the king of fruits for its crown and is the third most important tropical fruit after banana and citrus. The plant, which is indigenous to South America, is the most important species in the Bromeliaceae family and is largely traded for fresh fruit consumption. Here, we report the complete chloroplast sequence of the MD-2 pineapple that was sequenced using the PacBio sequencing technology. In this study, the high error rate of PacBio long sequence reads of A. comosus's total genomic DNA were improved by leveraging on the high accuracy but short Illumina reads for error-correction via the latest error correction module from Novocraft. Error corrected long PacBio reads were assembled by using a single tool to produce a contig representing the pineapple chloroplast genome. The genome of 159,636 bp in length is featured with the conserved quadripartite structure of chloroplast containing a large single copy region (LSC) with a size of 87,482 bp, a small single copy region (SSC) with a size of 18,622 bp and two inverted repeat regions (IRA and IRB) each with the size of 26,766 bp. Overall, the genome contained 117 unique coding regions and 30 were repeated in the IR region with its genes contents, structure and arrangement similar to its sister taxon, Typha latifolia. A total of 35 repeats structure were detected in both the coding and non-coding regions with a majority being tandem repeats. In addition, 205 SSRs were detected in the genome with six protein-coding genes contained more than two SSRs. Comparative chloroplast genomes from the subclass Commelinidae revealed a conservative protein coding gene albeit located in a highly divergence region. Analysis of selection pressure on protein-coding genes using Ka/Ks ratio showed significant positive selection exerted on the rps7 gene of the pineapple chloroplast with P less than 0.05. Phylogenetic analysis confirmed the recent taxonomical relation among the member of

  17. Effect of indirect ohmic heating on quality of ready-to-eat pineapple packed in plastic pouch

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    Hoang Pham

    2014-06-01

    Full Text Available Ready-to-eat fruits packed in sealed containers are highly perishable due to their intrinsic characteristics and lack of full thermal process. Ohmic heating has the advantages of rapid liquid heating through electrical current. The aim of this research was to investigate the effect of indirect ohmic heating on pH, total soluble solids, polyphenol oxidase activity, color and texture of ready-to-eat pineapple packed in a polypropylene pouch with 1% calcium chloride and 0.3% ascorbic acid packing solution. The pre-packed sample in a pouch was placed in the ohmic heating jar filled with 0.5% sodium chloride ohmic heating solution which was then ohmic heated at different voltage gradients (20, 30, 40 V/cm, to different packing solution temperatures (60, 70, 80°C for 60s. Samples were kept at 4°C for quality measurement. It was found that browning index of ready-to-eat pineapple treated with 20 V/cm at 80°C, 30 V/cm at 70°C and 80°C, 40 V/cm at 80°C did not change during 12 days cold storage (p>0.05. Polyphenol oxidase was inactivated when the temperature of the pineapple was 62°C or higher. After 10 days at 4°C, the pineapple heated with 30 V/cm at 70°C had much higher firmness than the un-heated sample kept at the same storage condition. Indirect ohmic heating of pre-packed ready-to-eat pineapple in polypropylene pouch with 30 V/cm at 70°C packing solution temperature for 60s could be used as minimal heating methods to maintain the quality of ready-to-eat fruits in 12 days at 4°C.

  18. ANTIMICROBIAL ACTIVITY OF PINEAPPLE (ANANAS COMOSUS L. MERR EXTRACT AGAINST MULTIDRUG-RESISTANT OF PSEUDOMONAS AERUGINOSA: AN IN VITRO STUDY

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    Rahmat Sayyid Zharfan

    2017-08-01

    Full Text Available Pseudomonas aeruginosa is the main cause of nosocomial infection which is responsible for 10% of hospital-acquired infection. Pseudomonas aeruginosa tends to mutate and displays potential for development of antibiotic resistance. Approximately, 10% of global bacterial isolates are found as Multidrug-resistant Pseudomonas aeruginosa. Pseudomonas aeruginosa have a quite tremendous severity index, especially on pneumonia and urinary tract infections, even sepsis, which 50% mortality rate. Pineapple (Ananas comosus L. Merr has antimicrobial properties. The active antimicrobial compounds in Ananas comosus L. Merr include saponin and bromelain. This research aims to find the potency of antimicrobial effect of pineapple (Ananas comosus L. Merr extract towards Multidrug-resistant Pseudomonas aeruginosa. Multidrug-resistant Pseudomonas aeruginosa specimen is obtained from patient’s pus in orthopaedic department, Dr Soetomo Public Hospital, Surabaya. Multidrug-resistant Pseudomonas aeruginosa specimen is resistant to all antibiotic agents except cefoperazone-sulbactam. This research is conducted by measuring the Minimum Inhibitory Concentration (MIC through dilution test with Mueller-Hinton broth medium. Pineapple extract (Ananas comosus L. Merr. is dissolved in aquadest, then poured into test tube at varying concentrations (6 g/ml; 3 g/ml; 1.5 g/ml; 0.75 g/ml, 0.375 g/ml; and 0.1875 g/ml. After 24 hours’ incubation, samples are plated onto nutrient agar plate, to determine the Minimum Bactericidal Concentration (MBC. The extract of pineapple (Ananas comosus L. Merr has antimicrobial activities against Multidrug-resistant Pseudomonas aeruginosa. Minimum Inhibitory Concentration (MIC could not be determined, because turbidity changes were not seen. The Minimum Bactericidal Concentration (MBC of pineapple extract (Ananas comosus L. Merr to Multidrug-resistant Pseudomonas aeruginosa is 0.75 g/ml. Further study of in vivo is needed.

  19. The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles.

    Science.gov (United States)

    Aragón, C; Pascual, P; González, J; Escalona, M; Carvalho, L; Amancio, S

    2013-11-01

    Proteomic and transcriptomic profiles of key enzymes were monitored in pineapple plants propagated under C3 and CAM-inducing metabolisms to obtain insight into the CAM-facultative metabolism and the relationship of CAM plants with oxidative stress. Pineapple is one of the most important tropical crops worldwide. The use of temporary immersion bioreactors for the first stages of pineapple propagation enables precise control of plant growth, increases the rate of plant multiplication, decreases space, energy and labor requirements for pineapple plants in commercial micropropagation. Once the plantlets are ready to be taken from the reactors, they are carefully acclimatized to natural environmental conditions, and a facultative C3/CAM metabolism in the first 2 months of growth is the characteristic of pineapple plants, depending on environmental conditions. We subjected two sets of micropropagated pineapple plants to C3 and CAM-inducing environmental conditions, determined by light intensity/relative humidity (respectively 40 μmol m−2 s−1/85 % and 260 μmol m−2 s−1/50 %). Leaves of pineapple plants grown under CAM-inducing conditions showed higher leaf thickness and more developed cuticles and hypodermic tissue. Proteomic profiles of several proteins, isoenzyme patterns and transcriptomic profiles were also measured. Five major spots were isolated and identified, two of them for the first time in Ananas comosus (OEE 1; OEE 2) and the other three corresponding to small fragments of the large subunit of Rubisco (LSU). PEPC and PEPCK were also detected by immunobloting of 2DE at the end of both ex vitro treatments (C3/CAM) during the dark period. Isoenzymes of SOD and CAT were identified by electrophoresis and the transcript levels of OEE 1 and CAT were associated with CAM metabolism in pineapple plants.

  20. Discovery of precursor and mature microRNAs and their putative gene targets using high-throughput sequencing in pineapple (Ananas comosus var. comosus).

    Science.gov (United States)

    Yusuf, Noor Hydayaty Md; Ong, Wen Dee; Redwan, Raimi Mohamed; Latip, Mariam Abd; Kumar, S Vijay

    2015-10-15

    MicroRNAs (miRNAs) are a class of small, endogenous non-coding RNAs that negatively regulate gene expression, resulting in the silencing of target mRNA transcripts through mRNA cleavage or translational inhibition. MiRNAs play significant roles in various biological and physiological processes in plants. However, the miRNA-mediated gene regulatory network in pineapple, the model tropical non-climacteric fruit, remains largely unexplored. Here, we report a complete list of pineapple mature miRNAs obtained from high-throughput small RNA sequencing and precursor miRNAs (pre-miRNAs) obtained from ESTs. Two small RNA libraries were constructed from pineapple fruits and leaves, respectively, using Illumina's Solexa technology. Sequence similarity analysis using miRBase revealed 579,179 reads homologous to 153 miRNAs from 41 miRNA families. In addition, a pineapple fruit transcriptome library consisting of approximately 30,000 EST contigs constructed using Solexa sequencing was used for the discovery of pre-miRNAs. In all, four pre-miRNAs were identified (MIR156, MIR399, MIR444 and MIR2673). Furthermore, the same pineapple transcriptome was used to dissect the function of the miRNAs in pineapple by predicting their putative targets in conjunction with their regulatory networks. In total, 23 metabolic pathways were found to be regulated by miRNAs in pineapple. The use of high-throughput sequencing in pineapples to unveil the presence of miRNAs and their regulatory pathways provides insight into the repertoire of miRNA regulation used exclusively in this non-climacteric model plant. Copyright © 2015 Elsevier B.V. All rights reserved.

  1. Determination of Sliced Pineapple Drying Characteristics in A Closed Loop Heat Pump Assisted Drying System

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    Cüneyt Tunçkal

    2018-02-01

    Full Text Available Pineapple (Ananascomosus slices were dried with the aid of a heat pump assisted dryer (HPD. During this process, air velocity was kept constant at 1m/s, while air temperatures were changed as 37°C, 40°C and 43°C. The drying air was also circulated by using an axial fan in a closed cycle and fresh air was not allowed into the system. The drying rate and drying time were significantly influenced by drying temperature. It was observed that drying temperatures had significant effects on the drying rate and drying time. During the conduct of the study, pineapple slices were dried at 37, 40 and 43°C for 465, 360 and 290 min, respectively. The specific moisture extraction ratio (SMER values were observed to change as drying temperatures were changed. The drying rate curves indicated that the whole drying process occurred in the falling rate period. Seven well-known thin-layer models (Lewis, Henderson &Pabis, Logarithmic, Page, Midilli & Kucuk, Weibull and Aghbashlo et al. were employed to make a prediction about drying kinetics through nonlinear regression analysis. The Midilli & Kucuk and Aghbashlo et al. models were consistent with the experimental data. Fick’s second law of diffusion was used to determine the moisture diffusivity coefficient ranging from 3.78×10–9 to 6.57×10-9  m2/s the each of the above mentioned temperatures. The dependence of effective diffusivity coefficient on temperature was defined by means a fan Arrhenius type equation. The activation energy of moisture diffusion was found to be 75.24kJ/mol.   Article History: Received: July 18th 2017; Received: October 27th 2017; Accepted: January 16th 2018; Available online How to Cite This Article: Tunçkal, C., Coşkun, S., Doymaz, I. and Ergun, E. (2018 Determination of Sliced Pineapple Drying Characteristics in A Closed Loop Heat Pump Assisted Drying System. International Journal of Renewable Energy Development, 7(1, 35-41. https://doi.org/10.14710/ijred.7.1.35-41

  2. Protective effect of pineapple (Ananas cosmosus peel extract on alcohol-induced oxidative stress in brain tissues of male albino rats

    Directory of Open Access Journals (Sweden)

    Ochuko L Erukainure

    2011-03-01

    Full Text Available Objective: To investigate the ability of pineapple peels to protect against alcohol-induced oxidative stress in brain tissues using male albino rat models. Methods: Response surface methodology (RSM was used to design a series of experiments to optimize treatment conditions with the aim of investigating the protective effect of pineapple peel extract on alcohol-induced oxidative stress in brain tissues. Oxidative stress was induced by oral administration of ethanol (20% w/v at a dosage of 5 mL/kg bw. The treatment lasted for 28 days. At the end of the treatment, the rats were fasted overnight and sacrificed by cervical dislocation. Tissue homogenates were used for the assessment of protein concentration, reduced glutathione (GSH content, catalase, and SOD. Results: Alcohol administration caused a significant decrease (P>0.05 in GSH level in the group which was only fed alcohol. Treatment with pineapple peel extracts caused increase in GSH level in alcohol fed groups. No significant difference (P<0.05 was observed in SOD levels of the negative control and group fed on only pineapple peel extract. Elevated level of catalase was observed in the negative control but pineapple peel extract significantly reduced the levels. Conclusions: This study indicates the protective effect of pineapple peel against alcoholinduced oxidative stress in brain tissues.

  3. Studies into the phenolic patterns of different tissues of pineapple (Ananas comosus [L.] Merr.) infructescence by HPLC-DAD-ESI-MS (n) and GC-MS analysis.

    Science.gov (United States)

    Steingass, Christof B; Glock, Mona P; Schweiggert, Ralf M; Carle, Reinhold

    2015-08-01

    In a comprehensive study, more than 60 phenolic compounds were detected in methanolic extracts from different tissues of pineapple infructescence by high-performance liquid chromatography with diode array detection and electrospray ionisation multiple-stage mass spectrometry (HPLC-DAD-ESI-MS (n) ) as well as by gas chromatography-mass spectrometry (GC-MS). The analytical workflow combining both methods revealed numerous compounds assigned for the first time as pineapple constituents by their mass fragmentations. Pineapple crown tissue was characterised by depsides of p-coumaric and ferulic acid. In contrast, major phenolic compounds in pineapple pulp extracts were assigned to diverse S-p-coumaryl, S-coniferyl and S-sinapyl derivatives of glutathione, N-L-γ-glutamyl-L-cysteine and L-cysteine, which were also identified in the peel. The latter was additionally characterised by elevated concentrations of p-coumaric, ferulic and caffeic acid depsides and glycerides, respectively. Two peel-specific cyanidin hexosides were found. Elevated concentrations of isomeric N,N'-diferuloylspermidines may be a useful tool for the detection of fraudulent peel usage for pineapple juice production. Mass fragmentation pathways of characteristic pineapple constituents are proposed, and their putative biological functions are discussed.

  4. The effect of pineapple waste (Ananas comosus (L. Merr subtitution on mixed basal diet of Elephant grass and calliandra on rumen ecosystem of sheep

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    Y. Widiawati

    2009-12-01

    Full Text Available The aim of this study was to evaluate the effect of pineapple waste substitution to mixed basal diet of Elephant grass and calliandra on rumen ecosystem. Pineapple waste was substituted to basal of Elephant grass and calliandra leaves (3:2 at the level of 0% (RA; 10% (RB; 20% (RC; 30% (RD; 40% (RE and 50% (RF. In this experiment 24 Indonesian local male sheep (9-10 months old, 15.3 kg average body weight were used, and were divided into 6 groups of dietary treatment (4 sheep each. Every group was offered one of the experimental diets (RA to RF in a Completely Randomized Design. Pineapple waste was offered gradually for one month until the level of 50% (RF was reached. The animals were adapted to experimental diets for about 14 days prior to the data collection period. Rumen fluids from each animal was taken (5 hours after morning feeding for pH, ammonia concentration; bacteria and protozoa population analysis. The results showed that substitution of pineapple waste up to 30% had no effect on pH, but when the level was increased up to 40 and 50%, the pH (P0.05. Increasing pineapple waste given increased population of amyllolytic bacteria but decreased the population of cellulolytic bacteria. On the Elephant grass and calliandra basal diet with the proportion of 3 : 2, the best substitution of pineapple waste was up to 20%.

  5. Preliminary results relating to gamma irradiations of the pollen in the pineapple (Ananas comosus (L.) Merr)

    International Nuclear Information System (INIS)

    Benaga, R.; Vicedo, L.; Isidron, M.; Arias, E.; Campanioni, L.; Fernandez, J.; Castillo, E.; Romero, M.

    1993-01-01

    As a way to know the radiosensitivity of the pollen of pineapple, in order to get to know haploid plants, the anthers of red Espanola of the type Pinarena and smooth Cayena from the Serrana (Ciego de Avila) were exposed to 60C o gamma radiations in the irradiator at CENSA, with fractional doses ranging from zero to 5KGy. The percentage of germination and length pollinic tube is analysed in vitro; a decrease in the above mentioned parameters is observed, with the main doses in the two cultures although red Espanola showed a slight recovery at doses of 3 and 4KGy as well as the major values of germination of the pollen

  6. Pollen-gamma irradiation on pineapple and pollination using irradiated pollen

    International Nuclear Information System (INIS)

    Benega, R.; Cisneros, A.; Martinez, J.; Arias, E.; Yabor, L.; Isidron, M.; Castillo, E.; Fernandez, J.

    1997-01-01

    In order to try to induce haploid plants, pineapple (Ananas comosus (L.) ;err.) pollen grains were gamma-rays irradiated with a Co 60 source at rates of doses from 0 to 300 Gy. The effect of gamma-rays on the generative-nucleus division and vitro pollen viability as well as, seed contents and plantlets obtained after pollinations was analysed. The were reducing in the division frequencies of generative nucleus at all assayed doses. The LD50 was achieved among 200 and 250 Gy. There were not differences on pollen viability in the rates of assayed doses. Seed contents were dose-dependent. The percentages of seed full and seed containing only embryos decreases at increasing of irradiation doses. Contrary effect was observed on the seed empty. Some plantlets regenerated from the different irradiation doses showed phenotypes with small and leaves and short inter nodes

  7. Improvement of pineapple (Ananas comosus (L.) Merr.) using biotechnology and mutation breeding techniques

    International Nuclear Information System (INIS)

    Osei-Kofi, F.; Amoatey, H.M.; Lokko, Y.

    1997-01-01

    Micropropagation and in vitro mutagenesis are reported in two local pineapple (Ananas comosus (L.) Merr.) varietie, 'Smooth Cayenne' and 'Sugar Loaf'. Multiple shoots developed on Murashige and Skoog medium containing 3.5% sucrose, 3μM/L thiamine HCl, 3μM/l naphthaline acetic acid (NAA) and varying concentrations of 6-benzylaminopurine (BAP). Shoot proliferation was best with 20 μM/l BAP. Shoots were rooted on MS medium supplemented with 1.5 μM/l indole-3-butyric acid (IBA) and 0.75 μM/l indole-3-acetic acid (IAA). Radiosensitivity was determined by irradiating in vitro shoot tips with 15 to 120 Gy gamma rays. The LD 50 was found to be 45 Gy, and doses above 80 Gy were lethal to explants. Projected methods are discussed to carry out mutation breeding for tolerance to drought and heat. (author). 12 refs, 2 figs

  8. Micropropagação do abacaxizeiro ornamental Protocol for in vitromicropropagation of ornamental pineapple

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    Moacir Pasqual

    2008-03-01

    Full Text Available O Ananas comosus var. erectifolius, cultivar de abacaxi ornamental, tem apresentado grande interesse para paisagistas e floricultores do Brasil e do exterior, por ser uma planta ornamental tropical, exótica e rústica. A produção de plantas ornamentais a partir de técnicas de cultura de tecidos apresenta-se como uma alternativa viável para a obtenção de um grande número de plantas com qualidade genética e fitossanitária, em um curto espaço de tempo, suprindo, assim, a necessidade do mercado na aquisição de mudas com qualidade comprovada. Estudou-se a influência das concentrações de BAP (0; 0,5; 1,0 e 1,5 mg L-1 e ANA (0,0; 0,12; 0,24; 0,48 mg L-1 no meio de cultura MS com 0; 2,5; 5,0; e 7,5 g L-1 de ágar, visando estabelecer um protocolo para multiplicação e enraizamento in vitro de brotos de abacaxizeiro ornamental. Brotações com 1,5 ± 0,5 cm, já estabelecidas in vitro, oriundas das gemas da coroa do fruto do abacaxizeiro ornamental, foram inoculados assepticamente nos frascos. Após inoculados, os explantes foram mantidos em sala de crescimento com luminosidade em torno de 35 ìmol m-2 s-1, 26±1ºC e fotoperíodo de 16 horas. Após 45 dias observou-se que a multiplicação in vitrodo abacaxi ornamental é viável em meio MS líquido acrescido de BAP 1,5 mg L-1 e o enraizamento também em meio MS líquido, na ausência de reguladores de crescimento.The Ananas comosus var. erectifoliusis an ornamental pineapple cultivar which greatly interests Brazilians and foreign landscapers and flower producers for being an exotic and rustic tropical ornamental plant. The market demand for high quality of cuttings requires efficient methods of propagation and in this context the tissue culture stands out as a viable alternative to obtain plants with genetic and phytossanitary quality in a short time. In the present work we studied the influence of concentrations of BAP (0; 0.5; 1.0; 1.5 mg L-1 and NAA (0; 0.12; 0.24; 0.48 mg L-1 in

  9. Improvement of pineapple (Ananas comosus (L.) Merr.) using biotechnology and mutation breeding techniques

    Energy Technology Data Exchange (ETDEWEB)

    Osei-Kofi, F; Amoatey, H M; Lokko, Y [Biotechnology and Nuclear Agriculture Research Inst., Ghana Atomic Energy Commission, Legon-Accra (Ghana)

    1997-07-01

    Micropropagation and in vitro mutagenesis are reported in two local pineapple (Ananas comosus (L.) Merr.) varietie, `Smooth Cayenne` and `Sugar Loaf`. Multiple shoots developed on Murashige and Skoog medium containing 3.5% sucrose, 3{mu}M/L thiamine HCl, 3{mu}M/l naphthaline acetic acid (NAA) and varying concentrations of 6-benzylaminopurine (BAP). Shoot proliferation was best with 20 {mu}M/l BAP. Shoots were rooted on MS medium supplemented with 1.5 {mu}M/l indole-3-butyric acid (IBA) and 0.75 {mu}M/l indole-3-acetic acid (IAA). Radiosensitivity was determined by irradiating in vitro shoot tips with 15 to 120 Gy gamma rays. The LD{sub 50} was found to be 45 Gy, and doses above 80 Gy were lethal to explants. Projected methods are discussed to carry out mutation breeding for tolerance to drought and heat. (author). 12 refs, 2 figs.

  10. Tapanuli Organoclay Addition Into Linear Low Density Polyethylene-Pineapple Fiber Composites

    International Nuclear Information System (INIS)

    Adawiyah, Robiatul; Juwono, Ariadne L.; Roseno, Seto

    2010-01-01

    Linear low density polyethylene-Tapanuli organoclay-pineapple fiber composites were succesfully synthesized by a melt intercalation method. The clay was modified as an organoclay by a cation exchange reaction using hexadecyl trimethyl ammonium bromide (HDTMABr) surfactant. The X-ray diffraction results of the organoclay exhibited a higher basal spacing of 1.87 nm compared to the unmodified clay of 1.46 nm. The composite tensile strength was enhanced up to 46.4% with the 1 wt% organoclay addition. Both tensile and flexural moduli increased up to 150.6% and 43% with the 3 wt% organoclay addition to the composites. However, the flexural strength of the composites was not improved with the organoclay addition. The addition of organoclay has also decreased the heat deflection temperature of the composites.

  11. Isolation and purification of bromelain from waste peel of pineapple for therapeutic application

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    Iara Rocha Antunes Pereira Bresolin

    2013-12-01

    Full Text Available The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%, followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, maintaining its enzymatic activity. The batch adsorption of bromelain was evaluated in terms of total protein and enzymatic activity using Langmuir and Langmuir-Freundlich models. Results showed that the process could be suitable for the recovery and purification of the enzyme, maintaining its specific activity.

  12. Simulating the Pineapple Express in the half degree Community Climate System Model, CCSM4

    Science.gov (United States)

    Shields, Christine A.; Kiehl, Jeffrey T.

    2016-07-01

    Atmospheric rivers are recognized as major contributors to the poleward transport of water vapor. Upon reaching land, these phenomena also play a critical role in extreme precipitation and flooding events. The Pineapple Express (PE) is defined as an atmospheric river extending out of the deep tropics and reaching the west coast of North America. Community Climate System Model (CCSM4) high-resolution ensemble simulations for the twentieth and 21st centuries are diagnosed to identify the PE. Analysis of the twentieth century simulations indicated that the CCSM4 accurately captures the spatial and temporal climatology of the PE. Analysis of the end 21st century simulations indicates a significant increase in storm duration and intensity of precipitation associated with landfall of the PE. Only a modest increase in the number of atmospheric rivers of a few percent is projected for the end of 21st century.

  13. Tapanuli Organoclay Addition Into Linear Low Density Polyethylene-Pineapple Fiber Composites

    Science.gov (United States)

    Adawiyah, Robiatul; Juwono, Ariadne L.; Roseno, Seto

    2010-12-01

    Linear low density polyethylene-Tapanuli organoclay-pineapple fiber composites were succesfully synthesized by a melt intercalation method. The clay was modified as an organoclay by a cation exchange reaction using hexadecyl trimethyl ammonium bromide (HDTMABr) surfactant. The X-ray diffraction results of the organoclay exhibited a higher basal spacing of 1.87 nm compared to the unmodified clay of 1.46 nm. The composite tensile strength was enhanced up to 46.4% with the 1 wt% organoclay addition. Both tensile and flexural moduli increased up to 150.6% and 43% with the 3 wt% organoclay addition to the composites. However, the flexural strength of the composites was not improved with the organoclay addition. The addition of organoclay has also decreased the heat deflection temperature of the composites.

  14. Insight into biosorption equilibrium, kinetics and thermodynamics of crystal violet onto Ananas comosus (pineapple) leaf powder

    Science.gov (United States)

    Chakraborty, Sagnik; Chowdhury, Shamik; Saha, Papita Das

    2012-06-01

    Biosorption performance of pineapple leaf powder (PLP) for removal of crystal violet (CV) from its aqueous solutions was investigated. To this end, the influence of operational parameters such as pH, biosorbent dose, initial dye concentration and temperature were studied employing a batch experimental setup. The biosorption process followed the Langmuir isotherm model with high correlation coefficients ( R 2 > 0.99) at different temperatures. The maximum monolayer biosorption capacity was found to be 78.22 mg g-1 at 293 K. The kinetic data conformed to the pseudo-second-order kinetic model. The activation energy of the system was calculated as 58.96 kJ mol- 1 , indicating chemisorption nature of the ongoing biosorption process. A thermodynamic study showed spontaneous and exothermic nature of the biosorption process. Owing to its low cost and high dye uptake capacity, PLP has potential for application as biosorbent for removal of CV from aqueous solutions.

  15. Effects of different deficit irrigation on sugar accumulation of pineapple during development

    Science.gov (United States)

    Feng, Haiyan; Du, Liqing; Liu, Shenghui; Zhang, Xiumei

    2017-08-01

    The potted pineapple cultivar ‘Comte de paris’ was used to study the influence of deficit irrigation on fruit sugar accumulation in greenhouse during the fruit enlargement period. The study included a control (normal irrigation) and two treatment groups, moderate deficit (50% of the control irrigation) and severe deficit (25% of the control irrigation). The results indicated that the deficit irrigation significantly decreased the sucrose accumulation. The sucrose content in the fruits of moderate deficit irrigation was the lowest. During the mature period, the deficit irrigation decreased the sucrose phosophate synthase activity(SPS) an increased the sucrose synthase (SS) and neutral invertase (NI). The moderate deficit irrigation significantly improved the acid invertase activity(AI). However, it was inhibited by the severe deficit irrigation. In general, the moderate treatment reduced the SPS activity and enhanced the NI and AI activities, while the severe treatment decreased the SPS and AI activities.

  16. Enhanced Production of Cellulase from Pineapple Waste by Response Surface Methodology

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    P. Saravanan

    2013-01-01

    Full Text Available Optimization of the media components for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using pineapple waste as substrate was performed with statistical methodology based on experimental designs. The screening of nutrients and their influence on the cellulase production was studied using a Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and yeast extract were found to have the positive influence for the production of cellulase. The selected components were optimized using response surface methodology. The optimum concentrations are avicel: 26.5 g/L, soybean cake flour: 22.5 g/L, KH2PO4: 4.5 g/L, and yeast extract: 12.3 g/L. A maximum cellulase activity of 8.61 IU/mL was obtained under the optimized medium in the validation experiment.

  17. Study of modification of fibers from pineapple crown for the formation of polymeric composites

    International Nuclear Information System (INIS)

    Marcon, Juliana S.; Mulinari, Daniella R.; Cioffi, Maria Odila H.; Voorwald, Herman J.C.

    2009-01-01

    Study of modification of fibers from pineapple crown for the formation of polymeric composites An important aspect to make fiber and matrix work together in a given application is the interface between them. For an efficient adherence fiber/matrix an appropriate interfacial contact is required. For this purpose, it was made a modification in the fiber surface using sodium hydroxide solution. And the effect of fibers modification was analyzed by X-Ray diffractometry (XRD) and scanning electron microscopy (SEM). The results indicated that occurred an effective increase in the crystallinity of modified fibers compared to natural fibers and that was occurred the formation of pores or holes across the rough surface of the fiber showing that will can occur an increase in effective superficial area for contact with polymeric matrix. (author)

  18. Do Labour Rights Matter for Export? A Qualitative Comparative Analysis of Pineapple Trade to the EU

    Directory of Open Access Journals (Sweden)

    Annelien Gansemans

    2017-12-01

    Full Text Available Labour norms are increasingly considered in trade relations, but is the protection of labour standards a necessary condition for export to the EU? A Qualitative Comparative Analysis, based on countries that export pineapples to the EU, shows that labour standards protection matters in combination with distance, zero tariffs and institutional quality in a number of cases. However, for none of the cases was it a sufficient condition on its own for determining exports to the European market. Rather, we show that (1 having a zero tariff is necessary for a relatively large share of export to the EU, and (2 labour standards protection can make a difference when the institutional quality is weak in some African cases, in contrast to Latin American exporters.

  19. Biosorption of Basic Green 4 from aqueous solution by Ananas comosus (pineapple) leaf powder.

    Science.gov (United States)

    Chowdhury, Shamik; Chakraborty, Sagnik; Saha, Papita

    2011-06-01

    Biosorption characteristics of Ananas comosus (pineapple) leaf powder was investigated for decolorization of Basic Green 4 (BG 4), a cationic dye from its aqueous solutions employing a batch experimental set-up. Parameters that influence the sorption process such as pH, biosorbent dosage, contact time, initial dye concentration and temperature were systematically studied. The optimum conditions for removal of BG 4 were found to be pH 9.0, contact time=150 min, biosorbent dosage=5.0 g L(-1), initial dye concentration=50 mg L(-1). The temperature had a strong influence on the biosorption process. Further, the biosorbent was characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and Brunauer, Emmett, Teller (BET) surface area and pore size analysis. Experimental biosorption data were modeled by Langmuir, Freundlich and Dubinin-Radushkevich (D-R) isotherms. The biosorption process followed the Langmuir isotherm model with high coefficients of correlation (R(2)>0.99) at different temperatures. The pseudo second order kinetic model fitted well in correlation to the experimental results. Activation energy of the biosorption process (E(a)) was found to be 45.79 kJ mol(-1) by using the Arrhenius equation, indicating chemisorption nature of BG 4 sorption onto pineapple leaf powder. Thermodynamic parameters suggest that the biosorption process is spontaneous and exothermic in nature. Overall, the present findings suggest that this environmentally friendly, efficient and low-cost biosorbent may be useful for the removal of BG 4 from aqueous media. Copyright © 2011 Elsevier B.V. All rights reserved.

  20. Cytokinins and auxin communicate nitrogen availability as long-distance signal molecules in pineapple (Ananas comosus).

    Science.gov (United States)

    Tamaki, Vívian; Mercier, Helenice

    2007-11-01

    This work aimed at identifying a possible role of phytohormones in long-distance (root-shoot) signaling under nitrogen deficiency. Three-months old pineapple plants were transferred from Murashige and Skoog (MS) medium to nitrogen-free MS (-N). During the first 24h on -N, 20 plants were harvested every 4h. After 30 days in -N, the remaining plants were transferred back to regular MS (+N) and 20 plants harvested every 4h for the first 24h. Following the harvests, endogenous levels of nitrate (NO(3)(-)), indole-3-acetic acid (IAA), isopentenyladenine (iP), isopentenyladenine riboside (iPR), zeatin (Z) and zeatin riboside (ZR) were analyzed in roots and leaves. In N-starved plants, the NO(3)(-) level dropped by 20% in roots between the first (4h) and the second harvest (8h). In leaves a reduction of 20% was found 4h later. Accumulation of IAA peaked in leaves at 16h. In roots, the accumulation of IAA only started at 16h while the leaf content was already in decline, which suggests that the hormone might have traveled from the leaves to the roots, communicating N-shortage. The contents of the four cytokinins were generally low in both, shoot and roots, and remained almost unchanged during the 24h of analysis. After N re-supply, roots showed a NO(3)(-) peak at 8h whereas the foliar concentration increased 4h later. Hormone levels in roots climaxed at 8h, this coinciding with the highest NO(3)(-) concentration. In leaf tissue, a dramatic accumulation was only observed for Z and ZR, and the peak was seen 4h later than in roots, suggesting that Z-type cytokinins might have traveled from the roots to the leaves. These findings provide evidence that there is a signaling pathway for N availability in pineapple plants, communicated upwards through cytokinins (N-supplemented plants) and downwards through auxin (N-starved plants).

  1. Enhanced iron and zinc accumulation in genetically engineered pineapple plants using soybean ferritin gene.

    Science.gov (United States)

    Mhatre, Minal; Srinivas, Lingam; Ganapathi, Thumballi R

    2011-12-01

    Pineapple (Ananas comosus L. Merr., cv. "Queen") leaf bases were transformed with Agrobacterium tumefaciens strain EHA 105 harboring the pSF and pEFESF plasmids with soybean ferritin cDNA. Four to eight percent of the co-cultivated leaf bases produced multiple shoots 6 weeks after transfer to Murashige and Skoog's medium supplemented with α-naphthalene acetic acid 1.8 mg/l, indole-3-butyric acid 2.0 mg/l, kinetin 2.0 mg/l, cefotaxime 400 mg/l, and kanamycin 50 mg/l. Putatively transformed shoots (1-2 cm) were selected and multiplied on medium of the same composition and elongated shoots (5 cm) were rooted on liquid rooting medium supplemented with cefotaxime 400 mg/l and kanamycin 100 mg/l. The rooted plants were analyzed through PCR, genomic Southern analysis, and reverse transcription PCR. The results clearly confirmed the integration and expression of soybean ferritin gene in the transformed plants. Atomic absorption spectroscopic analysis carried out with six independently transformed lines of pSF and pEFE-SF revealed a maximum of 5.03-fold increase in iron and 2.44-fold increase in zinc accumulation in the leaves of pSF-transformed plants. In pEFE-SF-transformed plants, a 3.65-fold increase in iron and 2.05-fold increase in zinc levels was observed. Few of the transgenic plants were hardened in the greenhouse and are being grown to maturity to determine the enhanced iron and zinc accumulation in the fruits. To the best of our knowledge this is the first report on the transformation of pineapple with soybean ferritin for enhanced accumulation of iron and zinc content in the transgenic plants.

  2. Mechanism of internal browning of pineapple: The role of gibberellins catabolism gene (AcGA2ox) and GAs

    Science.gov (United States)

    Zhang, Qin; Rao, Xiuwen; Zhang, Lubin; He, Congcong; Yang, Fang; Zhu, Shijiang

    2016-01-01

    Internal browning (IB), a physiological disorder (PD) that causes severe losses in harvested pineapple, can be induced by exogenous gibberellins (GAs). Over the years, studies have focused on roles of Gibberellin 2-oxidase (GA2oxs), the major GAs catabolic enzyme in plants, in the regulation of changes in morphology or biomass. However, whether GA2oxs could regulate PD has not been reported. Here, a full-length AcGA2ox cDNA was isolated from pineapple, with the putative protein sharing 23.59% to 72.92% identity with GA2oxs from five other plants. Pineapples stored at 5 °C stayed intact, while those stored at 20 °C showed severe IB. Storage at 5 °C enhanced AcGA2ox expression and decreased levels of a GAs (GA4) ‘compared with storage at 20 °C. However, at 20 °C, exogenous application of abscisic acid (ABA) significantly suppressed IB. ABA simultaneously upregulated AcGA2ox and reduced GA4. Ectopic expression of AcGA2ox in Arabidopsis resulted in reduced GA4, lower seed germination, and shorter hypocotyls and roots, all of which were restored by exogenous GA4/7. Moreover, in pineapple, GA4/7 upregulated polyphenol oxidase, while storage at 5 °C and ABA downregulated it. These results strongly suggest the involvement of AcGA2ox in regulation of GAs levels and a role of AcGA2ox in regulating IB. PMID:27982026

  3. Study on the physico-chemical properties, sensory attributes and shelf life of pineapple powder incorporated biscuits

    Directory of Open Access Journals (Sweden)

    M. Thivani

    2016-12-01

    Full Text Available The bakery industry is growing very fast and the products are becoming increasingly popular among consumers world-wide. Among ready-to-eat snacks, biscuits possess several attractive features including wider consumption base, relatively long shelf-life and preferred eating quality. Pineapples constitute important natural and valuable material in producing functional foods due to the presence of several anti-oxidants and bioactive compounds. Therefore, the aim of the present study was to investigate the nutritional composition and sensory quality of wheat flour biscuits incorporated with pineapple powder at the rates of 3, 5, 10, and 15% (w/w basis. The protein, fat, total sugar, moisture and mineral contents were significantly (p<0.05 changed during storage. Nutritional analysis indicated that biscuits incorporated with 5% pineapple powder had a slow rate of increase in moisture content (4.19 to 5.0% and decreasing trend in protein (22.15 to 22.06%, fat (18.83 to 18.79%, total sugar (58.52 to 58.34% and mineral contents (2.212 to 1.591% compared to other treatments during storage. Among the treatments, the biscuits prepared with 5% pineapple powder had the highest nutritional and sensory quality, having the overall acceptability score of 7.7 in a 9-point hedonic scale. The shelf life evaluation showed that these biscuits could be stored for 6 weeks at the ambient conditions of average temperature at 301ºC and RH at 75-80% with acceptable quality.

  4. The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions.

    Science.gov (United States)

    Aragón, C; Carvalho, L; González, J; Escalona, M; Amancio, S

    2012-04-01

    Many plant species grown under in vitro controlled conditions can be used as models for the study of physiological processes. Adult pineapple can display CAM physiology while in vitro it functions as a C3 plant. Ex vitro Ananas comosus has plastic morphology and physiology, both easy to modify from C3 to CAM by changing the environmental conditions. The yield of survival for a rentable propagation protocol of pineapple is closely related with the C3/CAM shift and the associated physiological characteristics. In the present work, ex vitro pineapple plants were divided in two sets and subjected to C3 and CAM-inducing environmental conditions, determined by light intensity and relative humidity, respectively, 40 μmol m(-2) s(-1)/85% and 260 μmol m(-2) s(-1)/50%. The results demonstrated that the stress imposed by the environmental conditions switched pineapple plants from C3 to CAM behavior. Comparing to CAM induced, C3-induced pineapple plants showed substandard growth parameters and morphological leaf characteristics but a better rooting process and a higher ABA production, a phenotype closer to adult plants, which are expected to produce fruits in a normal production cycle. We conclude that the upholding of these characteristics is conditioned by low light intensity plus high relative humidity, especially during the first 8 weeks of ex vitro growth. It is expected that the better understanding of pineapple acclimatization will contribute to the design of a protocol to apply as a rentable tool in the pineapple agronomic industry. © Springer-Verlag 2011

  5. Partitioning Carbon Dioxide Emission and Assessing Dissolved Organic Carbon Leaching of a Drained Peatland Cultivated with Pineapple at Saratok, Malaysia

    Directory of Open Access Journals (Sweden)

    Liza Nuriati Lim Kim Choo

    2014-01-01

    Full Text Available Pineapples (Ananas comosus (L. Merr. cultivation on drained peats could affect the release of carbon dioxide (CO2 into the atmosphere and also the leaching of dissolved organic carbon (DOC. Carbon dioxide emission needs to be partitioned before deciding on whether cultivated peat is net sink or net source of carbon. Partitioning of CO2 emission into root respiration, microbial respiration, and oxidative peat decomposition was achieved using a lysimeter experiment with three treatments: peat soil cultivated with pineapple, bare peat soil, and bare peat soil fumigated with chloroform. Drainage water leached from cultivated peat and bare peat soil was also analyzed for DOC. On a yearly basis, CO2 emissions were higher under bare peat (218.8 t CO2 ha/yr than under bare peat treated with chloroform (205 t CO2 ha/yr, and they were the lowest (179.6 t CO2 ha/yr under cultivated peat. Decreasing CO2 emissions under pineapple were attributed to the positive effects of photosynthesis and soil autotrophic activities. An average 235.7 mg/L loss of DOC under bare peat suggests rapid decline of peat organic carbon through heterotrophic respiration and peat decomposition. Soil CO2 emission depended on moderate temperature fluctuations, but it was not affected by soil moisture.

  6. MEDICINAL PLANTS AND HERBS OF NEWFOUNDLAND. PART 1. CHEMICAL CONSTITUENTS OF THE AERIAL PART OF PINEAPPLE WEED (Matricaria matricarioides

    Directory of Open Access Journals (Sweden)

    TIMOTHY F. LOOMIS

    2004-09-01

    Full Text Available The aerial part of Pineapple weed (Matricaria matricarioides, an adulterant of Chamomile, was investigated for its chemical constituents. Nine compounds were isolated and identified as two spiroethers [cis - en - yn - dicycloether 1 and trans - en - yn - dicycloether 2], three coumarins [7 - methoxycoumarin (Herniarin 3, umbelliferone 4 and 7 - methoxy - 3, 4 -dihydrocoumarin 5], phytol 6, luteolin - 7 - glucoside 7, (Z - 2 - β - D - Glucopyranosyloxyl - 4 - methoxycinnamic acid 8, and (E - 2 - β -D-Glucopyranosyloxyl - 4 -methoxycinnamic acid 9. By GC-MS analysis, the major components of the steam distilled volatile oil were identified as trans-en-yn-dicycloether and cis-en- yn-dicycloether, with the trans-form being more abundant than the cis-form. The results indicated some similarities between the constituents of Pineapple weed and those of German Chamomile. All these nine compounds are reported for the first time from Pineapple weed growing in Newfoundland. Compound 5 is reported from this plant genus for the first time.

  7. Particle passage kinetics and neutral detergent fiber degradability of silage of pineapple waste (aerial parts under different packing densities

    Directory of Open Access Journals (Sweden)

    Graciele Araújo de Oliveira Caetano

    2014-01-01

    Full Text Available The objective of this study was to determine the kinetics of in situ degradability parameters of the dry matter (DM and neutral detergent fiber (NDF and the passage of materials originating from the ensilage of the waste from pineapple cultivation (aerial parts. The four treatments utilized were silage of pineapple waste compacted at 600, 700, 900 and 1000 kg/m³. After ensiling the material from the pineapple cultivation, the particle-transit and rumen-degradation kinetics were analyzed. For the analysis of particle transit, chromium was utilized as a marker to mark the fiber. Passage rates were determined by retrieving the markers in the feces of the animals. In the degradation assay, samples were incubated in nylon bags for 0, 6, 18, 48 and 96 hours. The behavior observed in the regression curves of the variables analyzed describes high correlation between them, i.e., the time during which the silage is retained in the rumen influences its digestibility and its degradation rate. Although the silage compacted at 900 kg/m³ shows a larger potentially digestible fraction, it is recommended that it be ensiled at a compaction density of approximately 750 kg/m³ due to the lower cost and shorter mean retention time in the rumen-reticulum and rumen fill, thereby increasing the ruminal degradation and passage dynamics.

  8. Isolation of bacterial cellulose nanocrystalline from pineapple peel waste: Optimization of acid concentration in the hydrolysis method

    Science.gov (United States)

    Anwar, Budiman; Rosyid, Nurul Huda; Effendi, Devi Bentia; Nandiyanto, Asep Bayu Dani; Mudzakir, Ahmad; Hidayat, Topik

    2016-02-01

    Isolation of needle-shaped bacterial cellulose nanocrystalline with a diameter of 16-64 nm, a fiber length of 258-806 nm, and a degree of crystallinity of 64% from pineapple peel waste using an acid hydrolysis process was investigated. Experimental showed that selective concentration of acid played important roles in isolating the bacterial cellulose nanocrystalline from the cellulose source. To achieve the successful isolation of bacterial cellulose nanocrystalline, various acid concentrations were tested. To confirm the effect of acid concentration on the successful isolation process, the reaction conditions were fixed at a temperature of 50°C, a hydrolysis time of 30 minutes, and a bacterial cellulose-to-acid ratio of 1:50. Pineapple peel waste was used as a model for a cellulose source because to the best of our knowledge, there is no report on the use of this raw material for producing bacterial cellulose nanocrystalline. In fact, this material can be used as an alternative for ecofriendly and cost-free cellulose sources. Therefore, understanding in how to isolate bacterial cellulose nanocrystalline from pineapple peel waste has the potential for large-scale production of inexpensive cellulose nanocrystalline.

  9. A novel pineapple-structured Si/TiO{sub 2} composite as anode material for lithium ion batteries

    Energy Technology Data Exchange (ETDEWEB)

    Yan, Dong; Bai, Ying; Yu, Caiyan; Li, Xiaoge; Zhang, Weifeng, E-mail: wfzhang6@163.com

    2014-10-01

    Highlights: • Pineapple-structured Si/TiO{sub 2} composite was firstly synthesized by a simple sol–gel method. • Pineapple-structured Si/TiO{sub 2} composite exhibits the best cycling stability. • TiO{sub 2} layer not only effectively inhibits the volume change of Si, but also contributes its electrochemical activity. - Abstract: Nanoscaled Si is successfully wrapped with different contents of nano-TiO{sub 2} (the molar ratios of Si/TiO{sub 2} composites are 1:1, 1:2, 1:3, 1:4 and 1:5, respectively) to form a novel pineapple structure. X-ray diffraction (XRD), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM) and high resolution transmission electron microscopy (HRTEM) are utilized to characterize the structure, component, chemical environment and morphology of the composite. The investigation in cycling performances demonstrates that Si/TiO{sub 2} with molar ratio of 1:4 exhibits the best cycling stability, with specific capacity of 593 mA h g{sup −1} after 50 cycles at 0.1 C, much higher than those of the other composites and the pristine material. Cyclic voltammetry (CV) profiles are also measured and compared. It is believed that the outside TiO{sub 2} particles act as buffer against the huge volume change of Si during repeated alloying and de-alloying, which explains the improved electrochemical performances.

  10. Chemical profile of pineapple cv. Vitória in different maturation stages using electrospray ionization mass spectrometry.

    Science.gov (United States)

    Ogawa, Elizângela M; Costa, Helber B; Ventura, José A; Caetano, Luiz Cs; Pinto, Fernanda E; Oliveira, Bruno G; Barroso, Maria Eduarda S; Scherer, Rodrigo; Endringer, Denise C; Romão, Wanderson

    2018-02-01

    Pineapple is the fruit of Ananas comosus var. comosus plant, being cultivated in tropical areas and has high energy content and nutritional value. Herein, 30 samples of pineapple cv. Vitória were analyzed as a function of the maturation stage (0-5) and their physico-chemical parameters monitored. In addition, negative-ion mode electrospray ionization mass spectrometry [ESI(-)FT-ICR MS] was used to identify and semi-quantify primary and secondary metabolites present in the crude and phenolic extracts of pineapple, respectively. Physico-chemical tests show an increase in the total soluble solids (TSS) values and in the TSS/total titratable acidity ratio as a function of the maturity stage, where a maximum value was observed in stage 3 (¾ of the fruit is yellow, which corresponds to the color of the fruit peel). ESI(-)FT-ICR MS analysis for crude extracts showed the presence mainly of sugars as primary metabolites present in deprotonated molecule form ([M - H] - and [2 M - H] - ions) whereas, for phenolic fractions, 11 compounds were detected, being the most abundant in the third stage of maturation. This behavior was confirmed by quantitative analysis of total polyphenols. ESI-FT-ICR MS was efficient in identifying primary (carbohydrates and organic acids) and secondary metabolites (13 phenolic compounds) presents in the crude and phenolic extract of the samples, respectively. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  11. Effects of storage and packaging materials on some physicochemical properties and sensory and microbiological parameters of pineapple juice (ananas comosus)

    International Nuclear Information System (INIS)

    Shakpo, I.O.; Arawande, J.O.

    2010-01-01

    Physicochemical, microbiological and sensory parameters of concentrated pineapple juice stored in cans and glass bottles were studied over a period of ten weeks. There was slight increase in pH from 4.2 to 4.7 and to 4.8 and decrease in titratable acidity from 8.1 to 5.1 and to 4.6 mg/100 mL, whereas total solids (%) decreased from 76.23 to 65.47% and to 60.38% in canned and bottled pineapple concentrates, respectively. Over 90% loss of Vitamin C was observed, with the bottled samples retaining more Vitamin C than the canned samples. The microbial counts ranged from 2.0 X 103 to 2.4 X 104 cfu/mL whereas fungi and mesophilic bacteria, were not detected to 6 X 103 cfu/mL. Freshly prepared single strength juices of pineapple were better in terms of taste and colour, while the bottled reconstituted juice concentrate competed favourably with the fresh one in colour. The canned samples lost their colours within 10 weeks of storage. The glass bottled samples had a characteristic desirable aroma. Thus concentrated juice in glass bottles stored at room temperature enhanced the keeping quality of the juice and compared more favourably with the fresh juice than the canned concentrated juice. (author)

  12. Investigation of Endophytic Bacterial Community in Supposedly Axenic Cultures of Pineapple and Orchids with Evidence on Abundant Intracellular Bacteria.

    Science.gov (United States)

    Esposito-Polesi, Natalia Pimentel; de Abreu-Tarazi, Monita Fiori; de Almeida, Cristina Vieira; Tsai, Siu Mui; de Almeida, Marcílio

    2017-01-01

    Asepsis, defined as the absence of microbial contamination, is one of the most important requirements of plant micropropagation. In long-term micropropagated cultures, there may occasionally occur scattered microorganism growth in the culture medium. These microorganisms are common plant components and are known as latent endophytes. Thus, the aim of this research was to investigate the presence of endophytic bacteria in asymptomatic pineapple and orchid microplants, which were cultivated in three laboratories for 1 year. Isolation and characterization of bacterial isolates, PCR-DGGE from total genomic DNA of microplants and ultrastructural analysis of leaves were performed. In the culture-dependent technique, it was only possible to obtain bacterial isolates from pineapple microplants. In this case, the bacteria genera identified in the isolation technique were Bacillus, Acinetobacter, and Methylobacterium. The scanning electron microscopy and transmission electron microscopy (SEM and TEM) analyses revealed the presence of endophytic bacteria in intracellular spaces in the leaves of pineapple and orchid microplants, independent of the laboratory or cultivation protocol. Our results strongly indicate that there are endophytic bacterial communities inhabiting the microplants before initiation of the in vitro culture and that some of these endophytes persist in their latent form and can also grow in the culture medium even after long-term micropropagation, thus discarding the concept of "truly axenic plants."

  13. A novel pineapple-structured Si/TiO2 composite as anode material for lithium ion batteries

    International Nuclear Information System (INIS)

    Yan, Dong; Bai, Ying; Yu, Caiyan; Li, Xiaoge; Zhang, Weifeng

    2014-01-01

    Highlights: • Pineapple-structured Si/TiO 2 composite was firstly synthesized by a simple sol–gel method. • Pineapple-structured Si/TiO 2 composite exhibits the best cycling stability. • TiO 2 layer not only effectively inhibits the volume change of Si, but also contributes its electrochemical activity. - Abstract: Nanoscaled Si is successfully wrapped with different contents of nano-TiO 2 (the molar ratios of Si/TiO 2 composites are 1:1, 1:2, 1:3, 1:4 and 1:5, respectively) to form a novel pineapple structure. X-ray diffraction (XRD), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM) and high resolution transmission electron microscopy (HRTEM) are utilized to characterize the structure, component, chemical environment and morphology of the composite. The investigation in cycling performances demonstrates that Si/TiO 2 with molar ratio of 1:4 exhibits the best cycling stability, with specific capacity of 593 mA h g −1 after 50 cycles at 0.1 C, much higher than those of the other composites and the pristine material. Cyclic voltammetry (CV) profiles are also measured and compared. It is believed that the outside TiO 2 particles act as buffer against the huge volume change of Si during repeated alloying and de-alloying, which explains the improved electrochemical performances

  14. Effect of cassava starch coating on quality and shelf life of fresh-cut pineapple (Ananas comosus L. Merril cv "Pérola").

    Science.gov (United States)

    Bierhals, Vânia S; Chiumarelli, Marcela; Hubinger, Miriam D

    2011-01-01

    This research studied the influence of treatment with ascorbic acid, citric acid, and calcium lactate dipping and cassava starch edible coatings on quality parameters and shelf life of fresh-cut pineapple in slices during 12 d at 5 °C. After previous tests, the treatments selected for this study were samples dipped into antibrowning solution with 0.5% of ascorbic acid and 1% of citric acid, with and without 2% of calcium lactate and coated with 2% of cassava starch suspensions. Changes in weight loss, juice leakage, mechanical properties (stress at failure), color parameters (L* and H*), ascorbic acid content, sensory acceptance, and microbial growth of fruits were evaluated. Samples only treated with antibrowning agents were used as control. Edible coatings with and without calcium lactate were efficient in reducing weight loss, juice leakage, and maintaining firmness during storage. However, these samples showed more browning and the ascorbic acid content was reduced. All treatments presented good sensory acceptance (scores above 6). The determining factor of shelf life of pineapple slices was the microbial spoilage. A shelf life of 8 d was obtained for pineapple slices only treated with antibrowning agents. On the other hand, coated samples showed a reduced shelf life of 7 d and higher yeast and mold growth. Thus, although cassava starch coatings were efficient in reducing respiration rate, weight loss, and juice leakage and maintained mechanical properties, these treatments were not able to increase the shelf life of minimally processed pineapple. Practical Application: Pineapple fruit is highly appreciated for its aroma, flavor, and juiciness, but its immediate consumption is difficult. Therefore, pineapple is a potential fruit for minimal processing. However, shelf life of fresh-cut pineapple is very limited by changes in color, texture, appearance, off-flavors, and microbial growth. The use of edible coatings as gas and water vapor barrier and antibrowning

  15. Genome-Wide Identification and Expression Profiling of ATP-Binding Cassette (ABC) Transporter Gene Family in Pineapple (Ananas comosus (L.) Merr.) Reveal the Role of AcABCG38 in Pollen Development.

    Science.gov (United States)

    Chen, Piaojuan; Li, Yi; Zhao, Lihua; Hou, Zhimin; Yan, Maokai; Hu, Bingyan; Liu, Yanhui; Azam, Syed Muhammad; Zhang, Ziyan; Rahman, Zia Ur; Liu, Liping; Qin, Yuan

    2017-01-01

    Pineapple ( Ananas comosus L .) cultivation commonly relies on asexual reproduction which is easily impeded by many factors in agriculture production. Sexual reproduction might be a novel approach to improve the pineapple planting. However, genes controlling pineapple sexual reproduction are still remain elusive. In different organisms a conserved superfamily proteins known as ATP binding cassette (ABC) participate in various biological processes. Whereas, till today the ABC gene family has not been identified in pineapple. Here 100 ABC genes were identified in the pineapple genome and grouped into eight subfamilies (5 ABCAs , 20 ABCB s, 16 ABCCs , 2 ABCDs , one ABCEs , 5 ABCFs , 42 ABCGs and 9 ABCIs ). Gene expression profiling revealed the dynamic expression pattern of ABC gene family in various tissues and different developmental stages. AcABCA5, AcABCB6, AcABCC4 , AcABCC7 , AcABCC9 , AcABCG26 , AcABCG38 and AcABCG42 exhibited preferential expression in ovule and stamen. Over-expression of AcABCG38 in the Arabidopsis double mutant abcg1-2abcg16-2 partially restored its pollen abortion defects, indicating that AcABCG38 plays important roles in pollen development. Our study on ABC gene family in pineapple provides useful information for developing sexual pineapple plantation which could be utilized to improve pineapple agricultural production.

  16. Métodos de micropropagação de abacaxizeiro Micropropagation methods of pineapple

    Directory of Open Access Journals (Sweden)

    Adriano Bortolotti da Silva

    2007-09-01

    Full Text Available O objetivo deste trabalho foi comparar diferentes métodos de micropropagação in vitro de abacaxizeiro em larga escala. Os tratamentos utilizados foram micropropagação convencional em meio sólido (5 e 6 g L-1 de ágar e em meio líquido, e micropropagação em biorreator de imersão temporária - imersão no meio de cultura a cada 2, 4 e 8 horas, por 3 min - e contínua, com aeração a cada 2, 4 e 8 horas, por 3 min. Utilizou-se meio MS suplementado com 1 mg L-1 de BAP, 0,25 mg L-1 de ANA, pH ajustado para 5,8. As culturas foram mantidas em sala de crescimento com 25±1ºC, sob luz branca fria (40 µmol m-2 s-1 , com 16 horas de fotoperíodo. O delineamento foi inteiramente casualizado, com nove tratamentos e quatro repetições. Depois de 45 dias de cultivo, foram avaliados número de brotos, brotações superiores a 1 cm, comprimento de brotos, massa de matéria fresca e massa de matéria seca de brotos. Sistemas de imersão temporária, com as plântulas imersas a cada 2 horas por 3 min, proporcionaram maior número, altura e massa de matéria seca de brotos de abacaxizeiro. O sistema de imersão temporária é o método mais eficiente na micropropagação de abacaxizeiro em larga escala.The objective of this work was to compare different methods of pineapple in vitro mass micropropagation. The treatments were conventional micropropagation in solid medium (5 and 6 g L-1 agar and in liquid medium, micropropagation in bioreactor of temporary immersion (immersion in culture medium each 2, 4 and 8 hours, for 3 minutes and continuous immersion (with aeration each 2, 4 and 8 hours, for 3 minutes. MS medium supplement with 1 mg L-1 BAP, 0.25 mg L-1 NAA, and pH adjusted to 5.8 was utilized. Cultures were incubated at 25±1ºC under cool white fluorescent tubes (40 µmol m-2 s-1 with a 16-hours photoperiod. The experiment design was completely randomized, comprising nine treatments and four replicates. After 45 days, number of shootings

  17. Production of bioethanol from heart and pineapple shell using the yeast Saccharomyces Cerevisiae

    International Nuclear Information System (INIS)

    Corella Quiros, Byron Antonio

    2013-01-01

    The performance of bioethanol production was evaluated from heart and pineapple shell, using the yeast Saccharomyces Cerevisiae, in which has been obtained a maximum output of 1,6% v/v. The research was divided into a phase of characterization and five experimental phases. The heart and pineapple shell were used as substrate for the study. The contents of glucose, reducing sugars and total, moisture, ash, crude fiber and soluble solids content were determined of the heart and golden pineapple shell (MD2). The shell has had a higher content of soluble solids, fiber content, ash and lower moisture content and reducing sugars. In the first experimental phase was made a fermentation of commercial sucrose, with the objective to corroborate the method of measurement of CO 2 and the pH was measured of the water that is collected the gas. Great variation between samples has not been observed, comparing the method to estimate the losses of gas, so it is reproducible and the losses of CO 2 has been at least of 22%. In the second experimental stage to compare measurement methods of ethanol, for collection of CO 2 and gas chromatography, it has been found that for concentrations from 0 to 0,79% v/v, the results have shown a quadratic behavior (second-degree polynomial with 0,83173x 2 +0,0024 x, R 2 =0,9984), while that for higher concentrations to 0,79% the relation has been linear (0,6372 x -0,099, R 2 =0,9424), in which x is the %v/v of ethanol, of the chromatographic method. In the third experimental stage were compared the effects of the filtration. The significant differences of this effect were not found for either of the two substrates used: hearts and shells. The adjustment parameters of the modified Gompertz equation for mixtures of 53% heart and 47% shell, and concentration of 280 g/L have been: Pm 0,72 %v/v; λ 0,3 h, Rm 0,047 (%v/v)/h; for a concentration of 400 g/L, have been Pm 1,3 %v/v λ 1,8 h and Rm 0,068 (%v/v)/h and for 523 g/L, using extract of yeast have

  18. Mobilidade do boro em plantas de abacaxi Boron mobility in pineapple

    Directory of Open Access Journals (Sweden)

    Susana Cristine Siebeneichler

    2005-08-01

    Full Text Available Para confirmar a mobilidade do boro em abacaxizeiro, cultivaram-se doze mudas da cultivar 'Pérola' em solução nutritiva completa, contendo 1 µmol L-1 de B, em delineamento inteiramente casualizado. Após 45 dias, coletaram-se quatro plantas (T0 e, nas oito plantas restantes, aplicou-se, por 3 dias consecutivos, uma solução de H3BO3 a 10 mmol L-1, pincelando-se ambas as faces da folha basal número sete (7. A partir do início da aplicação foliar, foi suspenso o fornecimento de B na solução nutritiva. Um dia após a terceira aplicação foliar, coletaram-se quatro plantas (T1 e, 60 dias após esta, coletaram-se as últimas quatro plantas (T2. As plantas foram fracionadas em diferentes partes nas quais o teor de B total foi determinado pelo método da azometina-H. Em T0, o maior teor de B observado foi na raiz; no T1, na folha pincelada e na raiz e, no T2, na folha pincelada. O conteúdo de B acompanhou a variação da massa seca das porções da planta, sendo que, no T2, o maior conteúdo de B e massa seca foram observados nas folhas novas formadas entre o T1 e o T2. O boro contido nessas folhas (29,8% do boro total da planta proveio das folhas mais velhas (FMed, FTrat e FBas, cujo conteúdo diminuiu significativamente, confirmando a mobilidade do boro em plantas de abacaxi 'Pérola'.To confirm the mobility of boron in pineapple, twelve 'Pérola' pineapple seedlings were cultivated in a complete nutrient solution containing 1 mmol L-1 B in a completely randomized design. After 45 days, B was removed from the nutrient solution, and four plants were harvested (T0, and in the eight remaining plants 10 mmol L-1 H3BO3 solution were painted, for 3 consecutive days, on both faces of the basal leaf number seven (7. One day after the third foliar application, four plants were harvested (T1 and, sixty days later, the last four plants were harvested (T2. The plants were partitioned into different parts in which total B concentration was determined

  19. Effects of pineapple byproduct and canola oil as fat replacers on physicochemical and sensory qualities of low-fat beef burger.

    Science.gov (United States)

    Selani, Miriam M; Shirado, Giovanna A N; Margiotta, Gregório B; Saldaña, Erick; Spada, Fernanda P; Piedade, Sonia M S; Contreras-Castillo, Carmen J; Canniatti-Brazaca, Solange G

    2016-02-01

    Pineapple byproduct and canola oil were evaluated as fat replacers on physicochemical and sensory characteristics of low-fat burgers. Five treatments were performed: conventional (CN, 20% fat) and four low-fat formulations (10% fat): control (CT), pineapple byproduct (PA), canola oil (CO), pineapple byproduct and canola oil (PC). Higher water and fat retention and lower cooking loss and diameter reduction were found in burgers with byproduct addition. In raw burgers, byproduct incorporation reduced L*, a*, and C* values, but these alterations were masked after cooking, leading to products similar to CN. Low-fat treatments were harder, chewier, and more cohesive than full-fat burgers. However, in Warner Bratzler shear measurements, PA and PC were as tender as CN. In QDA, no difference was found between CN and PC. Pineapple byproducts along with canola oil are promising fat replacers in beef burgers. In order to increase the feasibility of use of pineapple byproduct in the meat industry, alternative processes of byproduct preparation should be evaluated in future studies. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Genetic characterization of natural populations of pineapple guava (Acca sellowiana, with heterologous microsatellites markers

    Directory of Open Access Journals (Sweden)

    Karine Louise dos Santos

    2011-12-01

    Full Text Available Pineapple guava (Acca sellowiana is a native species from south Brazil and northeast Uruguay, and due to the unique flavor of its fruits, it is an income-generating alternative to small farmers. Knowledge on genetic diversity is an important tool for genetic improvement and conservation. Aiming to increase the knowledge with regarde to the species genetic diversity, fi ve natural populations of A. sellowiana were analyzed through microsatellites markers developed from Eucalyptus grandis W. Hill ex Maiden x E. urophylla S.T. Blake complex. Using 10 pairs of selected markers, 122 plants were characterized. The mean values for expected and observed heterozigosity were 0.42 and 0.47, respectively. The fˆ estimates did not differ from zero to four out of the five populations evaluated, suggesting a small inbreeding effect. In addition, private alleles and high genetic divergence was observed. the average genetic divergence among the populations was st Fˆ = 0,13 e st Rˆ = 0,14, mostly due to the incidenceof rare or exclusive alleles among some populations.

  1. The effect of pineapple core fiber on dough rheology and the quality of mantou

    Directory of Open Access Journals (Sweden)

    Sy-Yu Shiau

    2015-09-01

    Full Text Available The consumption of dietary fiber offers the health benefit of lowering the risk of many chronic diseases. Pineapple core fiber (PCF in this study was extracted and incorporated into dough and mantou (i.e., steamed bread. The effects of PCF substitution and fiber size on textural and rheological properties of dough and mantou were evaluated by a texture analyzer. The substitution of wheat flour by PCF resulted in a stiffer and less extensible dough with or without fermentation. The hardness and gumminess of mantou significantly increased as the PCF substitution increased from 0% to 15%, but the cohesiveness, specific volume, and elasticity significantly decreased with the fiber substitution. Ten percent PCF-enriched dough and mantou with various fiber sizes had similar rheological and textural properties, except for the k1 and k2 values. By sensory evaluation, 5% PCF-enriched mantou and the control bread had better acceptability in texture, color, odor, and overall acceptability, compared to mantous enriched with 10% or 15% PCF. Significant correlations existed between the rheological properties of dough and textural parameters of mantou and between the sensory quality and textural parameters of mantou. Therefore, we suggest that fiber-enriched mantou can be prepared with 5% PCF substitution to increase the intake of dietary fiber and maintain the quality of mantou.

  2. Cattle management practices and milk production on mixed smallholder organic pineapple farms in Central Uganda

    DEFF Research Database (Denmark)

    Nalubwama, S; Kabi, F; Vaarst, M

    2016-01-01

    A longitudinal study to assess animal management practices and milk production was conducted for a period of 12 months on 30 smallholder farms keeping dairy cattle and certified organic pineapple production in Luwero and Kayunga districts, based on questionnaire and on-farm collected data. Farm...... sizes were 9.3 ± 6.7 acres in tethering system and 4.3 ± 2.6 acres in zero-grazing. Fifty-four percent of the zero-grazing herds had animal housing facilities. All farmers in tethering system kept cows on earthen floors and calves without bedding. Hygiene level in existing farms was low. Majority...... of calves were fed once a day by restricted suckling (77 %). Seventy-four percent of tethered cows were only fed on natural grass, while cows under zero-grazing system had a more diversified diet but with 82 % feeding mainly Napier grass. Most farms (87 %) used bulls for breeding. Milk production was higher...

  3. Mechanical and optical characterization of bio-nanocomposite from pineapple leaf fiber material for food packaging

    Science.gov (United States)

    Nikmatin, Siti; Rudwiyanti, Jerry R.; Prasetyo, Kurnia W.; Yedi, Dwi A.

    2015-01-01

    The utilization of Bio-nanocomposite material that was derived from pineapple leaf fiber as filler and tapioca starch with plasticizer glycerol as a matrix for food packaging can reduce the use of plastic that usually was made from petroleum materials. It is important to develop and producethis environmental friendly plastic because of limited availability of petroleum nowadays. The process of synthesize and characterization tapioca starch with the plasticizer glycerol bionanocomposites using print method had been conducted. There were 3 samples with different filler concentration variation; 3%, 4% and 5%.The results of mechanical test from each sample showed that bio-nanocomposite with 5% filler concentration was the optimum sample with 4.6320 MPa for tensile strength test and 24.87% for the elongation test. Based on the result of optical test for each sample was gained that along with the increasing of concentration filler would make the absorbance value of the sample became decreased, bio-nanocomposite with 5% filler concentration had several peaks with low absorbance values. The first peak was in 253 nm of wavelength regionwith absorbance of 0.131%, and the second peak was in 343 nmwavelength region and absorbance was 0.087%.

  4. Pineapple juice labeled with gadolinium: a convenient oral contrast for magnetic resonance cholangiopancreatography

    International Nuclear Information System (INIS)

    Coppens, Emmanuel; Metens, Thierry; Winant, Catherine; Matos, Celso

    2005-01-01

    The aim of our study was to prepare in vitro a pineapple juice (PJ) solution labeled with a minimal gadolinium concentration working as a negative contrast agent in heavily T2-weighted imaging and to assess that solution in vivo as a negative oral contrast agent for magnetic resonance cholangiopancreatography (MRCP). Three PJs were compared in vitro according to their T2. Increasing concentrations of gadolinium (Gd)-DOTA in PJ were assessed in vitro for T2 reduction. Single-shot turbo spin echo T2-weighted MR cholangiopancreatograms were obtained for 35 patients with suspected biliopancreatic duct disease, before and after ingestion of the PJ/Gd-DOTA solution. Signal intensity (SI) measurements of gastroduodenal lumens, pancreatobiliary ducts, and image quality scores were obtained systematically before and after contrast ingestion. The in vitro selected Gd-DOTA concentration in the PJ was 2.76 mmol/l. Ingestion of 180 ml of PJ labeled with 1 ml of Gd-DOTA eliminated efficiently the gastroduodenal SI in MRCP, improving significantly the rates of complete visualization of the pancreatobiliary ducts (P<0.01) and the MRCP image quality scores (P<0.05). All patients easily ingested the contrast solution and found the solution palatable. PJ labeled with gadolinium constituted an efficient and convenient negative oral contrast agent for MRCP. (orig.)

  5. Evaluation of antibacterial activities of silver nanoparticles green-synthesized using pineapple leaf (Ananas comosus).

    Science.gov (United States)

    Emeka, Elemike Elias; Ojiefoh, Oseghale Charles; Aleruchi, Chuku; Hassan, Labulo Ayomide; Christiana, Owoseni Mojisola; Rebecca, Mfon; Dare, Enock Olugbenga; Temitope, Adesuji Elijah

    2014-02-01

    Pineapple leaf was used in this study for the synthesis of silver nanoparticles based on the search for sustainable synthetic means. Indeed, this offered an economical and sustainable synthetic route relative to expensive and toxic chemical methods. The leaf extract was used and the corresponding nanoparticles obtained were subjected to UV-vis analysis at different times. The UV-vis was used to monitor the silver nanoparticle formation through sampling at time intervals. The formation of silver nanoparticles was apparently displayed within 2 min with evidence of surface plasmon bands (SPB) between 440 and 460 nm. The crystals was equally characterized using FTIR, X-ray diffraction methods and TEM. The different results obtained suggested the appearance of silver nanoparticles (SNPs) as determined by the process parameters with a particle size of 12.4 nm. The sample was further screened against Staphylococcus aureus, Streptococcus pneumoniae, Proteus mirabilis and Escherichia coli using Gentamicin as control. From the results, there is evidence of inhibition towards bacteria growth. It can now be inferred from the studies that biosynthesis of nanoparticles could be a gateway to our numerous health issues. Copyright © 2013 Elsevier Ltd. All rights reserved.

  6. Adhesion of pineapple-leaf fiber to epoxy matrix: The role of surface treatments

    Directory of Open Access Journals (Sweden)

    Yusran Payae

    2009-07-01

    Full Text Available Natural fibers are considered to have potential use as reinforcing agents in polymer composite materials because of their principle benefits: moderate strength and stiffness, low cost, and be an environmental friendly, degradable, and renewablematerial. Due to their inherently hydrophilic nature, they are prone to absorb moisture, which can plasticise or weaken theadhesion of fibers to the surrounding matrix and by this affect the performance of composites used in atmospheric humidity,particularly at elevated temperatures. The surface treatments are often applied to the fiber to improve the bond strengthbetween the fibers and matrix. This work discussed the effect of sodium hydroxide (NaOH treatment and epoxy resin as acompatibilizing agent on interface properties of pineapple leaf fiber (PALF-epoxy composites. A single-fiber fragmentationtest coupled with data reduction technique was employed to assess interface quality in terms of apparent interfacial shearstrength (IFSS or a of untreated, NaOH, and epoxy resin treated PALFs-epoxy composites. Tensile properties of untreatedand treated PALFs were also examined. It was found that both treatments substantially increase a, corresponding to animproved level of adhesion. The improvement in the level of adhesion for the alkali and epoxy treated fiber composites wasdue to an increase in the physical bonding between the alkali treated fibers and the matrix, and due to a promoted compatibilitybetween the epoxy treated fibers and matrix, respectively.

  7. Nanoporous carbon derived from agro-waste pineapple leaves for supercapacitor electrode

    Science.gov (United States)

    Sodtipinta, Jedsada; Amornsakchai, Taweechai; Pakawatpanurut, Pasit

    2017-09-01

    By using KOH as the chemical activating agent in the synthesis, the activated carbon derived from pineapple leaf fiber (PALF) was prepared. The structure, morphology, and the surface functional groups of the as-prepared activated carbon were investigated using x-ray diffraction, field emission scanning electron microscope equipped with energy dispersive x-ray spectroscopy, and x-ray photoelectron spectroscopy. The electrochemical behavior and performance of the as-synthesized activated carbon electrode were measured using the cyclic voltammetry and the electrochemical impedance spectroscopy in 1 M Na2SO4 electrolyte solution in three-electrode setup. The activated carbon electrode exhibited the specific capacitance of 131.3 F g-1 at a scan rate of 5 mV s-1 with excellent cycling stability. The capacitance retention after 1000 cycles was about 97% of the initial capacitance at a scan rate of 30 mV s-1. Given these good electrochemical properties along with the high abundance of PALF, this activated carbon electrode has the potential to be one of the materials for future large-scale production of the electrochemical capacitors. Invited talk at 5th Thailand International Nanotechnology Conference (Nano Thailand-2016), 27-29 November 2016, Nakhon Ratchasima, Thailand.

  8. Physical properties of coir and pineapple leaf fibre reinforced polylactic acid hybrid composites

    Science.gov (United States)

    Siakeng, R.; Jawaid, M.; Ariffin, H.; Sapuan, S. M.

    2018-01-01

    This study examined the physical behaviour of Coir fibres (CF)/Pineapple leaf fibres (PALF)/Poly lactic acid (PLA) composites. In this research, coir and PALF reinforced PLA hybrid composites were fabricated by hand lay-up process and hot press. The aim of this work is to do comparative study on density, water absorption (WA) and thickness swelling (TS) of untreated CF/PALF reinforced PLA composites and hybrid composites. The effect of different fibre ratios in hybridization on density, WA and TS of CF/PALF hybrid composites were also analyzed and C7P3 showed highest density while P30 had lowest. The results indicated that the density varies on different fibre ratio. WA and TS of CF/PALF composites and hybrid composites vary with fibres ratio and soaking duration. WA and TS of untreated CF/PALF hybrid composites were increased by increasing coir fibre ratio so, C30 showed highest WA and TS whereas P30 and C1P1 showed least WA and TS respectively apart from neat PLA.

  9. Pineapple juice labeled with gadolinium: a convenient oral contrast for magnetic resonance cholangiopancreatography

    Energy Technology Data Exchange (ETDEWEB)

    Coppens, Emmanuel; Metens, Thierry; Winant, Catherine; Matos, Celso [Hopital Erasme, Universite Libre de Bruxelles, Department of Radiology, Division of Magnetic Resonance, Brussels (Belgium)

    2005-10-01

    The aim of our study was to prepare in vitro a pineapple juice (PJ) solution labeled with a minimal gadolinium concentration working as a negative contrast agent in heavily T2-weighted imaging and to assess that solution in vivo as a negative oral contrast agent for magnetic resonance cholangiopancreatography (MRCP). Three PJs were compared in vitro according to their T2. Increasing concentrations of gadolinium (Gd)-DOTA in PJ were assessed in vitro for T2 reduction. Single-shot turbo spin echo T2-weighted MR cholangiopancreatograms were obtained for 35 patients with suspected biliopancreatic duct disease, before and after ingestion of the PJ/Gd-DOTA solution. Signal intensity (SI) measurements of gastroduodenal lumens, pancreatobiliary ducts, and image quality scores were obtained systematically before and after contrast ingestion. The in vitro selected Gd-DOTA concentration in the PJ was 2.76 mmol/l. Ingestion of 180 ml of PJ labeled with 1 ml of Gd-DOTA eliminated efficiently the gastroduodenal SI in MRCP, improving significantly the rates of complete visualization of the pancreatobiliary ducts (P<0.01) and the MRCP image quality scores (P<0.05). All patients easily ingested the contrast solution and found the solution palatable. PJ labeled with gadolinium constituted an efficient and convenient negative oral contrast agent for MRCP. (orig.)

  10. Effect of Pineapple Leaf Fibers (PALF) concentration on nanofibers formation by electrospinning

    Science.gov (United States)

    Surip, S. N.; Aziz, F. M. Abdul; Bonnia, N. N.; Sekak, K. A.

    2018-01-01

    Electrospinning method has been studied widely in producing nanofibers due to its straightforward and versatile method. In this study, Pineapple Leaf Fibers (PALF) solution were electrospinning to obtain mat of PALF electrospun. PALF were diluted in Trifluoacetic Acid (TFA) into five different concentrations to study the effect of concentration to the nanofibers formation. Raw sample of PALF (PALFraw), PALF after dewax (PALFdewax) and PALF after dilute with TFA (PALFTFA) were analyzed and compared using FTIR to study the structural change occur. TFA solvent has removed and recreated some of the functional group in PALF thus disrupt strong hydrogen bonds that hold hemicellulose, cellulose and lignin together. All the PALF sample has been proceed to electrospinning process. Low concentration of solution cause the solution jet to break up even before reach the collector however high concentration of solution made the solvent volatile faster and the solution dried easily. Therefore, PALF with optimum concentration of 0.02 gml-1 had favors the formation of nanofibers and succeed in forming membrane at the collector.

  11. Removal of methylene blue from aqueous solution by adsorption onto pineapple leaf powder

    International Nuclear Information System (INIS)

    Weng, Chih-Huang; Lin, Yao-Tung; Tzeng, Tai-Wei

    2009-01-01

    The ability of an unconventional bio-adsorbent, pineapple leaf powder (PLP) for the adsorption of methylene blue (MB) from aqueous solution was studied. It was observed that intra-particle diffusion was involved in the adsorption process and that the kinetic data fitted well with a pseudo-second-order equation. Fitting parameters revealed that the rate of adsorption increased with decrease in dye concentration and decrease in ionic strength while the mixing speed did not have a significant effect on adsorption. The adsorption was favorable at higher pH and lower temperature, and the equilibrium data were well fitted by the Langmuir isotherm. The maximum adsorption capacity varied from 4.68 x 10 -4 to 9.28 x 10 -4 mol/g when pH increases from 3.5 to 9.5. Thermodynamic parameters suggest that the adsorption is a typical physical process, spontaneous, and exothermic in nature. The results revealed that this agricultural waste has potential to be used as an economical adsorbent for the removal of methylene blue from aqueous solution.

  12. Isolation and characterization of a FLOWERING LOCUS T homolog from pineapple (Ananas comosus (L.) Merr).

    Science.gov (United States)

    Lv, LingLing; Duan, Jun; Xie, JiangHui; Wei, ChangBin; Liu, YuGe; Liu, ShengHui; Sun, GuangMing

    2012-09-01

    FLOWERING LOCUS T (FT)-like genes are crucial regulators of flowering in angiosperms. A homolog of FT, designated as AcFT (GenBank ID: HQ343233), was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The cDNA sequence of AcFT is 915 bp in length and contains an ORF of 534 bp, which encodes a protein of 177 aa. Molecular weight was 19.9 kDa and isoelectric point was 6.96. The deduced protein sequence of AcFT was 84% and 82% identical to homologs encoded by CgFT in Cymbidium goeringii and OgFT in Oncidium Gower Ramsey respectively. Quantitative real-time PCR (qRT-PCR) analyses showed that the expression of AcFT was high in flesh and none in leaves. qRT-PCR analyses in different stages indicated that the expression of AcFT reached the highest level on 40 d after flower inducing, when the multiple fruit and floral organs were forming. The 35S::AcFT transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants. Copyright © 2012 Elsevier B.V. All rights reserved.

  13. Structural characterization of a glucuronoarabinoxylan from pineapple (Ananas comosus (L.) Merrill) gum exudate.

    Science.gov (United States)

    Simas-Tosin, Fernanda F; de Souza, Lauro M; Wagner, Ricardo; Pereira, Graciele C Z; Barraza, Ruth R; Wendel, Cinthia F; Sassaki, Guilherme L; Iacomini, Marcello; Gorin, Philip A J

    2013-04-15

    Native polysaccharide from pineapple gum (PANP) was obtained following alkaline extraction of gum and fractionation with cetylpyridinium chloride. It was characterized as a glucuronoarabinoxylan using NMR, methylation data, controlled Smith degradation, carboxy-reduction, and ESI-MS of oligosaccharides produced on mild acid hydrolysis of PANP. HSPEC-MALLS-RI of carboxy-reduced fraction showed homogeneous profile (Mw 1.943×10(5) g/mol). PANP was composed of Ara, Xyl, Gal, and GlcpA (40:23:7:30 molar ratio). Its main chain presented (1→4)-linked β-xylan, highly substituted at O-2 and O-3 by side chains of 3-O- and 3,5-di-O-linked α-Araf, 2-O- and 4-O-linked α-GlcpA, and nonreducing end-units of α-Araf, β-Arap, β-Galp, and α-GlcpA. ESI-MS of a mixture of oligosaccharides formed on the mild acid hydrolysis of PANP was consistent with repetitive structures of α-GlcpA O-3 linked at β-Xylp units, whereas in others glucuronoarabinoxylan-type gum exudates, α-GlcpA units had been previously found to be linked at O-2. Copyright © 2013 Elsevier Ltd. All rights reserved.

  14. Removal of methylene blue from aqueous solution by adsorption onto pineapple leaf powder

    Energy Technology Data Exchange (ETDEWEB)

    Weng, Chih-Huang, E-mail: chweng@isu.edu.tw [Department of Civil and Ecological Engineering, I-Shou University, Da-Hsu Township, Kaohsiung 84008, Taiwan (China); Lin, Yao-Tung; Tzeng, Tai-Wei [Department of Soil and Environmental Sciences, National Chung Hsing University, TaiChung 40227, Taiwan (China)

    2009-10-15

    The ability of an unconventional bio-adsorbent, pineapple leaf powder (PLP) for the adsorption of methylene blue (MB) from aqueous solution was studied. It was observed that intra-particle diffusion was involved in the adsorption process and that the kinetic data fitted well with a pseudo-second-order equation. Fitting parameters revealed that the rate of adsorption increased with decrease in dye concentration and decrease in ionic strength while the mixing speed did not have a significant effect on adsorption. The adsorption was favorable at higher pH and lower temperature, and the equilibrium data were well fitted by the Langmuir isotherm. The maximum adsorption capacity varied from 4.68 x 10{sup -4} to 9.28 x 10{sup -4} mol/g when pH increases from 3.5 to 9.5. Thermodynamic parameters suggest that the adsorption is a typical physical process, spontaneous, and exothermic in nature. The results revealed that this agricultural waste has potential to be used as an economical adsorbent for the removal of methylene blue from aqueous solution.

  15. Relationships between some Thai cultivars of pineapple (Ananas comosus revealed by RAPD analysis

    Directory of Open Access Journals (Sweden)

    Siam Popluechai

    2007-11-01

    Full Text Available RAPD analysis of nine Thai pineapple cultivars, including 'Phulae', 'Sawee', 'Tradsithong', 'Phuket', 'Pattavia', 'Intrachitdang', 'Intrachitkhow', 'Petburi No.1', and 'Nanglae', showed that, of 40 arbitrary 10- mer primers, 17 primers gave 206 DNA fragments ranging from 510 to 4,700 bp. One hundred and forty-five (70.4% of the amplified fragments were polymorphic. RAPD analysis using NTSYS-pc Version 2.01e also showed that the similarity coefficients among the cultivars were 0.643-0. 963. The dendrogram indicated that the cultivars were clustered into 3 groups, consistent with the morphological data. The first group, consisting of 'Phuket', 'Phulae', 'Tradsithong', 'Sawee', and 'Petburi No.1', had morphological characteristics of the Queen group, while those of the second ('Intrachitdang' and 'Intrachitkow' and the third ('Nanglae' and 'Pattavia' groups could be determined morphologically to be members of the Spanish and Cayenne groups, respectively. 'Intrachitdang' and 'Intrachitkow' have similarity coefficient of 0.963, while that of 'Phulae' and 'Phuket' is 0.950. These pairs of cultivars are probably the same cultivars. The morphological differences between them are probably caused by mutations, differences in environment and agricultural practices, or combinations of these factors.

  16. Characterization of Phosphoenolpyruvate Carboxykinase from Pineapple Leaves Ananas comosus (L.) Merr. 1

    Science.gov (United States)

    Daley, Laurence S.; Ray, Thomas B.; Vines, H. Max; Black, Clanton C.

    1977-01-01

    Phosphoenolpyruvate carboxykinase has been partially purified from pineapple (Ananas comosus [L.]) leaves. Specific activities obtained show it to be a major activity in this tissue. Above 15 C, the respective activation energies for decarboxylation and carboxylation are 13 and 12 kcal/mol. Below 15 C, there are discontinuities in Arrhenius plots with an associated large increase in activation energy. The adenine nucleotides are preferred to other nucleotides as substrates. The apparent Km values in the carboxylation direction are: ADP 0.13 mm, HCO3- 3.4 mm, and phosphoenolpyruvate 5 mm. In the decarboxylation direction, the apparent Km values are: ATP 0.02 mm, ADP 0.05 mm, and oxaloacetate 0.4 mm. The decarboxylation activity had an almost equal velocity with either ADP or ATP. The pH optima are between 6.8 and 7. Inhibition of the carboxylation reaction by ATP, pyruvate, and carbonic anhydrase was demonstrated. Decarboxylase specific activities are over twice carboxylation activities. The data support a model in which phosphoenolpyruvate carboxykinase is of physiological significance only during the light period and then only as a decarboxylase. PMID:16659905

  17. Characterization of Phosphoenolpyruvate Carboxykinase from Pineapple Leaves Ananas comosus (L.) Merr.

    Science.gov (United States)

    Daley, L S; Ray, T B; Vines, H M; Black, C C

    1977-04-01

    Phosphoenolpyruvate carboxykinase has been partially purified from pineapple (Ananas comosus [L.]) leaves. Specific activities obtained show it to be a major activity in this tissue. Above 15 C, the respective activation energies for decarboxylation and carboxylation are 13 and 12 kcal/mol. Below 15 C, there are discontinuities in Arrhenius plots with an associated large increase in activation energy. The adenine nucleotides are preferred to other nucleotides as substrates. The apparent Km values in the carboxylation direction are: ADP 0.13 mm, HCO(3) (-) 3.4 mm, and phosphoenolpyruvate 5 mm. In the decarboxylation direction, the apparent Km values are: ATP 0.02 mm, ADP 0.05 mm, and oxaloacetate 0.4 mm. The decarboxylation activity had an almost equal velocity with either ADP or ATP. The pH optima are between 6.8 and 7. Inhibition of the carboxylation reaction by ATP, pyruvate, and carbonic anhydrase was demonstrated. Decarboxylase specific activities are over twice carboxylation activities. The data support a model in which phosphoenolpyruvate carboxykinase is of physiological significance only during the light period and then only as a decarboxylase.

  18. Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

    Directory of Open Access Journals (Sweden)

    Nadjma Souza Leite

    2012-10-01

    Full Text Available This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7. Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL while in the Imperial cultivar, it was higher in the stem after eight months (0.0179 U/mL. Imperial cultivar showed higher bromelain activity than the Pérola's.

  19. The study of mechanical properties of pineapple leaf fibre reinforced tapioca based bioplastic resin composite

    Directory of Open Access Journals (Sweden)

    Mathivanan D.

    2016-01-01

    Full Text Available Natural fibre reinforced composite has brought the material engineering to a high new level of research. Natural fibres are compatible with matrices like polypropylene and can be used as reinforcement material to reduce the composition of plastic in a material. Natural fibres such as kenaf, pineapple leaf, and coir already found its importance in reducing the dependence of petroleum based products. However the biodegradability of the product at the end of the intended lifespan is still questionable. This has led many researches to look for a suitable replacement for synthetic fibres and achieve better adhesion between fibre and matrix. In this study, fiber and matrix which are hydrophilic in nature was used and the mixture was extruded and hot compressed to acquire better mechanical properties. The specimens were fabricated and tested according to ASTM D638. The 30% composition illustrates the best average modulus value among other composition and from this result it can be concluded that the increase of PALF fibre in TBR composite increases the modulus strength of the composite.

  20. Characterisation and potential application of pineapple pomace in an extruded product for fibre enhancement.

    Science.gov (United States)

    Selani, Miriam Mabel; Brazaca, Solange Guidolin Canniatti; Dos Santos Dias, Carlos Tadeu; Ratnayake, Wajira S; Flores, Rolando A; Bianchini, Andreia

    2014-11-15

    This study characterised pineapple pomace (PP) and evaluated its application in extrusion to enhance fibre content of the final product. The pomace had low fat (0.61%) and high dietary fibre (45.22%), showing its potential for fibre enrichment of nutritionally poor products, as some extruded snacks. Results also showed low microbiological counts, water activity, and pH indicating good microbiological quality and low risk of physicochemical deterioration. During extrusion, pomace (0%, 10.5% and 21%), moisture (14%, 15% and 16%) and temperature (140 and 160°C) were evaluated. The PP addition decreased expansion and luminosity; while increasing redness of the extrudates compared to the control (0% pomace/14% moisture/140°C). When hardness, yellowness, water absorption, and bulk density were compared to the control, there was no effect (p>0.05) of 10.5% PP addition on the extrudates, indicating that, at this level, PP could be added without affecting the properties of the final extruded product. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. On-line gas chromatography combustion/pyrolysis isotope ratio mass spectrometry (HRGC-C/P-IRMS) of pineapple (Ananas comosus L. Merr.) volatiles.

    Science.gov (United States)

    Preston, Christina; Richling, Elke; Elss, Sandra; Appel, Markus; Heckel, Frank; Hartlieb, Ariane; Schreier, Peter

    2003-12-31

    By use of extracts prepared by liquid-liquid separation of the volatiles from self-prepared juices of pineapple fruits (Ananas comosus) (n = 14) as well as commercial pineapple recovery aromas/water phases (n = 3), on-line capillary gas chromatography-isotope ratio mass spectrometry was employed in the combustion (C) and the pyrolysis (P) modes (HRGC-C/P-IRMS) to determine the delta(13)C(VPDB) and delta(2)H(VSMOW) values of selected pineapple flavor constituents. In addition to methyl 2-methylbutanoate 1, ethyl 2-methylbutanoate 2, methyl hexanoate 3, ethyl hexanoate 4, and 2,5-dimethyl-4-methoxy-3[2H]-furanone 5, each originating from the fruit, the delta(13)C(VPDB) and delta(2)H(VSMOW) data of commercial synthetic 1-5 and "natural" (biotechnologically derived) 1-4 were determined. With delta(13)C(VPDB) data of pineapple volatiles 1-4 varying from -12.8 to -24.4 per thousand, the range expected for CAM metabolism was observed. Compound 5 showed higher depletion from -20.9 to -28.6 per thousand. A similar situation was given for the delta(2)H(VSMOW) values of 3-5 from pineapple ranging from -118 to -191 per thousand, whereas 1 and 2 showed higher depleted values from -184 to -263 per thousand. In nearly all cases, analytical differentiation of 1-5 from pineapple and natural as well as synthetic origin was possible. In general, natural and synthetic 1-5 exhibited delta(13)C(VPDB) data ranging from -11.8 to -32.2 per thousand and -22.7 to -35.9 per thousand, respectively. Their delta(2)H(VSMOW) data were in the range from -242 to -323 per thousand and -49 to -163 per thousand, respectively.

  2. Inactivation of Escherichia coli, Listeria monocytogenes, and Salmonella Enteritidis by Cymbopogon citratus D.C. Stapf. Essential Oil in Pineapple Juice.

    Science.gov (United States)

    Leite, Caroline Junqueira Barcellos; de Sousa, Jossana Pereira; Medeiros, José Alberto da Costa; da Conceição, Maria Lúcia; dos Santos Falcão-Silva, Vivyanne; de Souza, Evandro Leite

    2016-02-01

    In the present study, the efficacy of Cymbopogon citratus D.C. Stapf. essential oil (CCEO) to provoke a 5-log CFU/ml (5-log) inactivation in a mixed composite of Escherichia coli, Listeria monocytogenes, and Salmonella enterica serovar Enteritidis in pineapple (Ananas comosus (L.) Merril) juice (4°C) was assessed. Moreover, the effects of CCEO on the physicochemical and sensory quality parameters of pineapple juice were evaluated. The MIC of CCEO was 5 μl/ml against the composite mix examined. For L. monocytogenes and E. coli inoculated in juice containing CCEO (5, 2.5, and 1.25 μl/ml), a ≥5-log reduction was detected after 15 min of exposure. This same result was obtained for Salmonella Enteritidis incubated alone in pineapple juice containing CCEO at 5 and 2.5 μl/ml. Overall, Salmonella Enteritidis was the most tolerant and L. monocytogenes was the most sensitive to CCEO. The physicochemical properties (pH, titratable acidic [citric acid per 100 g], and soluble solids) of pineapple juice containing CCEO (2.5 and 1.25 μl/ml) were maintained. Juice containing CCEO (2.5 and 1.25 μl/ml) exhibited similar scores for odor, appearance, and viscosity compared with juice without CCEO. However, unsatisfactory changes in taste and aftertaste were observed in juices containing CCEO. These results suggest that CCEO could be used as an alternative antimicrobial compound to ensure the safety of pineapple juice, although CCEO at the tested concentrations negatively impacted its taste. Therefore, further studies are needed to determine the balance between microbial safety and taste acceptability of pineapple juice containing CCEO.

  3. Management and use of dairy cattle feed resources on smallholder certified organic pineapple farms in Central Uganda

    Directory of Open Access Journals (Sweden)

    Muhammad Kiggundu

    2014-12-01

    Full Text Available Formulation of exclusively organic diets that meet maintenance and production requirements of dairy cattle is a major limitation to production of premium organic products of animal origin. This study was therefore carried out to assess the use and availability of feed resources and the coping strategies used by farmers to overcome dry season feed shortages on 64 smallholder certified organic pineapple farms. Data was collected using semi-structured questionnaires and two focus group discussions. Majority of households were headed by males (62.9% while average age of respondents was 42.5 years. Farmers allocated more land (P<0.05 to organic pineapple production compared to livestock. Beside dairy cattle, farmers also kept chickens, goats and pigs. Tethering was the commonest cattle management system. Fifty three percent of respondents reported using both natural pastures and crop residues as major dairy cattle feed resources while only 19% reported using elephant grass. Banana peels (25.1% and sweet potato vines (24.7% were the most important crop residues fed to cattle. Farmers reported high cost of concentrates and scarcity of feeds as their biggest challenges in dairy cattle production. Of the respondents, 51.4% conserved feed for their cattle as fodder banks. As a coping strategy to feed shortages, majority (42.9% of farmer scavenged for feed resources from both organic certified and nonorganic neighbouring farms which is contrary to organic livestock farming standards. It was, therefore, concluded that management of livestock feeding in the study area fell short of the requirements for organic livestock feeding standards. Research to develop strategies that can use alternative on-farm feed resources through ensiling organic pineapple wastes during the dry season is recommended as a long term strategy to address feed challenges for organic livestock farmers.

  4. Molecular characterization and pathogenicity of Erwinia spp. associated with pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L.

    Directory of Open Access Journals (Sweden)

    Ramachandran Kogeethavani

    2015-12-01

    Full Text Available The Erwinia species are well-known pathogens of economic importance in Malaysia causing serious damage to high-value fruit crops that include pineapple [Ananas comosus (L. Merr.] and papaya (Carica papaya L..The 16S rRNA sequence using eubacteria fD1 and rP2 primers, identified two bacteria species; Dickeya zeae from pineapple heart rot, and Erwinia mallotivora from papaya dieback. Phylogenetic analysis based on the neighbor-joining method indicated that all the bacterial isolates clustered in their own taxa and formed monophyletic clades. From the pathogenicity test, all isolates of D. zeae and E. mallotivora showed pathogenic reactions on their respective host plants. Genetic variability of these isolates was assessed using repetitive sequence-based PCR (rep-PCR fingerprinting. The results indicated interspecies, and intraspecies variation in both species’ isolates. There were more polymorphic bands shown by rep-PCR fingerprints than enterobacterial repetitive intergenic consensus (ERIC and BOX- PCRs, however both species’ isolates produced distinguishable banding patterns. Unweighted pair-group method with arithmetic averages (UPGMA cluster analysis indicated that all Dickeya and Erwinia isolates from the same species were grouped in the same main cluster. Similarity among the isolates ranged from 77 to 99%. Sequencing of 16S rRNA using eubacteria fD1 and rP2 primers, and rep-PCR fingerprinting revealed diversity among Dickeya and Erwinia isolates. But this method appears to be reliable for discriminating isolates from pineapple heart rot and papaya dieback.

  5. The role of aqueous extract of pineapple fruit parts on the healing of acute crush tendon injury.

    Science.gov (United States)

    Aiyegbusi, A I; Duru, F I O; Awelimobor, D; Noronha, C C; Okanlawon, A O

    2010-01-01

    The Pineapple plant contains the enzyme bromelain which has been acclaimed to reduce pain and swellings following acute muscle injuries as well as carotenoids and polyphenols which are powerful antioxidants. It is yet to be determined if these constituents are distributed throughout the plant and what effect they have on the healing of acute tendon injuries. This study therefore investigated the effects of the aqueous extract of different parts of the pineapple plant on tenoblast proliferation and the tendon Malondialdehyde (MDA) level in the early stage of healing in a crush injury to the achilles tendon of Sprague-Dawley rats. Forty male rats were divided randomly into five groups; all had induced crush injury to the left Achilles tendon. Group 1; injury and nil treatment, Group 2; leaves extract, Group 3; fruit flesh extract, Group 4; bark extract, Group 5; core extract. The extract was given at a dosage of 30 mg/kg body weight daily over the first 14 days post-injury. On the 15th day post injury, the animals were sacrificed and the tendons excised and processed for histological study and MDA assay. The flesh and bark extract induced a proliferation of tenoblasts which however was not significantly different from that of the untreated tendon while the leaves and core extracts reduced the population of the tenocytes. The flesh extract significantly (p leaves and core extract significantly (p pineapple plant are concentrated in the flesh while the bark and flesh extracts have the potential to promote healing by stimulating tenoblast proliferation.

  6. THE EFFECT OF PICLORAM AND LIGHT ON SOMATIC EMBRYOGENESIS REGENERATION OF PINEAPPLE

    Directory of Open Access Journals (Sweden)

    Ika Roostika

    2012-10-01

    Full Text Available Smooth Cayenne is the largest pineapple type cultivated in Indonesia, but its vegetative planting materials for mass propagation are limited. Somatic embryogenesis is a potential method to be applied. The aim of this study was to investigate the somatic embryogenesis regeneration under the effect of picloram and light. Callus formation was induced by picloram (21, 41 and 62 μM added with 9 μM thidiazuron. The calli were transferred onto MS or Bac medium  enriched with N-organic compounds with or without addition of 21 μM picloram under dark or light condition. The compact calli were subcultured onto MS medium supplemented with 4.65 μM kinetin, while the friable calli were  transferred onto BIG medium (modified MS + 1.1 μM benzyl adenine + 0.9 μM indole butyric acid + 0.09 μM giberelic acid or B medium (MS + 0.018 mM benzyl adenine. The results showed that the events of somatic embryogenesis were started from cell polarization, asymmetrical division, proembryo formation as  embryogenic tissues and friable embryogenic tissues, and embryo development. The best treatment for callus induction was 21 μM picloram. The addition of 21 μM picloram on N-organic enriched medium and the use of light condition  proliferated embryogenic calli. The N-organic enriched Bac medium and light condition yielded the highest number of mature somatic embryos (17 embryos perexplant in 2 months. The B medium was better than BIG medium to develop  somatic embryos from friable embryogenic tissues. The somatic embryogenesis method presented is potential for pineapple mass propagation and artificial seedproduction.Abstrak Bahasa IndonesiaSmooth Cayenne merupakan kultivar nenas yang banyak dibudidayakan di  Indonesia, namun ketersediaan benih untuk perbanyakan massal masih terbatas. Embriogenesis somatikadalah metode yang potensial untuk produksi bibit secara massal. Tujuan penelitian adalah untuk mempelajari pengaruh pikloram dan pencahayaan terhadap regenerasi

  7. Conservation of minimally processed pineapple using calcium chloride, edible coating and gamma radiation

    International Nuclear Information System (INIS)

    Pilon, Lucimeire

    2007-01-01

    The aim of this study was to obtain a convenience type pineapple subjected to fresh-cut process and calcium chloride, wheat gluten and alginate-base edible coating and irradiation treatments. The fruits were washed, sanitized with Sumaveg (Sodium Dichloro-s-Triazinetrione) in a 200 mg L-1 chlorine-free solution at 7 deg C for 15 minutes, and then manually peeled. The peeled fruits were sliced into 1 cm thick slices, rinsed in 20 mg L-1 chlorine-free solution for 3 minutes and drained for 3 minutes. In the first experiment, the samples were treated with: 1% calcium chloride + vital wheat gluten solution; 1% calcium chloride + 1% alginate solution; and control. In the second experiment, the samples were treated with: 1% calcium chloride + vital wheat gluten solution + 2.3 kGy; 1% calcium chloride + 2.3kGy; irradiation with 2.3kGy; and control. The packing consisted of rigid polyethylene terephthalate (PET) trays with around 250 g of fruit. The irradiation was performed in a Cobalt-60 multipurpose irradiator with 92 kCi activity and dose value of 2.3 kGy h-1. The samples were stored at 5 ± 1 deg C and evaluated every other day for 12 days. In the first experiment pH and titratable acidity values showed slight variations but were similar between the treatments. There was a decrease in ascorbic acid values in all treatments. Browning was noticed in all treatments over the storage period. Although the values between the treatments were similar, the pineapple treated with calcium chloride + gluten showed firmer texture, less liquid loss, and lower values of polyphenoloxidase and peroxidase activities and CO 2 and ethylene production. Mesophiles and mold and yeast counts were also reduced. No Salmonella and E. coli were detected. Total coliform counts were low in all the treatments and appeared in just a few isolated samples during the storage period. Sensory analyses showed that the samples treated with calcium chloride + gluten had the lower scores for texture

  8. Destabilization of Human Insulin Fibrils by Peptides of Fruit Bromelain Derived From Ananas comosus (Pineapple).

    Science.gov (United States)

    Das, Sromona; Bhattacharyya, Debasish

    2017-12-01

    Deposition of insulin aggregates in human body leads to dysfunctioning of several organs. Effectiveness of fruit bromelain from pineapple in prevention of insulin aggregate was investigated. Proteolyses of bromelain was done as par human digestive system and the pool of small peptides was separated from larger peptides and proteins. Under conditions of growth of insulin aggregates from its monomers, this pool of peptides restricted the reaction upto formation of oligomers of limited size. These peptides also destabilized preformed insulin aggregates to oligomers. These processes were followed fluorimetrically using Thioflavin T and 1-ANS, size-exclusion HPLC, dynamic light scattering, atomic force microscopy, and transmission electron microscopy. Sequences of insulin (A and B chains) and bromelain were aligned using Clustal W software to predict most probable sites of interactions. Synthetic tripeptides corresponding to the hydrophobic interactive sites of bromelain showed disaggregation of insulin suggesting specificity of interactions. The peptides GG and AAA serving as negative controls showed no potency in destabilization of aggregates. Disaggregation potency of the peptides was also observed when insulin was deposited on HepG2 liver cells where no formation of toxic oligomers occurred. Amyloidogenic des-octapeptide (B23-B30 of insulin) incapable of cell signaling showed cytotoxicity similar to insulin. This toxicity could be neutralized by bromelain derived peptides. FT-IR and far-UV circular dichroism analysis indicated that disaggregated insulin had structure distinctly different from that of its hexameric (native) or monomeric states. Based on the stoichiometry of interaction and irreversibility of disaggregation, the mechanism/s of the peptides and insulin interactions has been proposed. J. Cell. Biochem. 118: 4881-4896, 2017. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  9. Cloning, localization and expression analysis of vacuolar sugar transporters in the CAM plant Ananas comosus (pineapple).

    Science.gov (United States)

    Antony, Edna; Taybi, Tahar; Courbot, Mikaël; Mugford, Sam T; Smith, J Andrew C; Borland, Anne M

    2008-01-01

    In photosynthetic tissues of the CAM plant pineapple (Ananas comosus), storage of soluble sugars in the central vacuole during the daytime and their remobilization at night is required to provide carbon skeletons for nocturnal CO(2) fixation. However, soluble sugars produced photosynthetically must also be exported to support growth processes in heterotrophic tissues. To begin to address how vacuolar sugar storage and assimilate partitioning are regulated in A. comosus, degenerate PCR and cDNA library screening were used to clone three candidate sugar transporters from the leaves of this species. Subcellular localization of the three transporters was investigated via expression of YFP-fusion proteins in tobacco epidermal cells and their co-localization with subcellular markers by confocal microscopy. Using this strategy, a putative hexose transporter (AcMST1) and a putative inositol transporter (AcINT1) were identified that both localized to the tonoplast, whereas a putative sucrose transporter (AcSUT1) was found to localize to prevacuolar compartments. A cDNA (AcMST2) with high similarity to a recently characterized tonoplast hexose transporter in Arabidopsis was also identified from an A. comosus fruit EST database. Analyses of transcript abundance indicated that AcMST1 was more highly expressed in fruits compared to leaves of A. comosus, whilst transcripts of AcINT1, AcSUT1, and AcMST2 were more abundant in leaves. Transcript abundance of AcINT1, the putative inositol transporter, showed day-night changes comparable to those of other CAM-related transcripts described in Mesembryanthemum crystallinum. The results are discussed in terms of the role of vacuolar sugar transporters in regulating carbon flow during the diel cycle in CAM plants.

  10. Cloning and expression analysis of a PISTILLATA homologous gene from pineapple (Ananas comosus L. Merr).

    Science.gov (United States)

    Lv, Ling-Ling; Duan, Jun; Xie, Jiang-Hui; Liu, Yu-Ge; Wei, Chang-Bin; Liu, Sheng-Hui; Zhang, Jian-Xia; Sun, Guang-Ming

    2012-01-01

    PISTILLATA (PI)-like genes are crucial regulators of flowering in angiosperms. A homologue of PI, designated as AcPI (Genbank accession number HQ717796), was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The cDNA sequence of AcPI is 907 bp in length and contains an open reading frame of 594 bp, which encodes a protein of 197 amino acids. The molecular weight was 2.29 kDa and the isoelectric point was 9.28. The alignment showed that AcPI had a high identity with CsPIC2 (78.6%), AoPI (77.4%), OrcPI (75.7%) and HPI2 (72.4%). Quantitative real-time polymerase chain reaction (qRT-PCR) analyses in different tissues showed that the expression pattern of AcPI was different from the B-class genes in eudicots. AcPI was expressed in all the tissues investigated. The expression level was very low in fruit stems, bracts, leaves and sepals, high in petals and carpels, and moderate in apical meristems, flesh and stamens. The qRT-PCR analyses in different stages indicated that the expression of AcPI reached the highest level at 40 days after flower inducement, when the multiple fruit and floral organs were forming. It proved the important role of AcPI in floral organs and fruit development. The 35S::AcPI transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants.

  11. Cloning and Expression Analysis of a PISTILLATA Homologous Gene from Pineapple (Ananas comosus L. Merr

    Directory of Open Access Journals (Sweden)

    Guang-Ming Sun

    2012-01-01

    Full Text Available PISTILLATA (PI-like genes are crucial regulators of flowering in angiosperms. A homologue of PI, designated as AcPI (Genbank accession number HQ717796, was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR and rapid amplification of cDNA ends (RACE. The cDNA sequence of AcPI is 907 bp in length and contains an open reading frame of 594 bp, which encodes a protein of 197 amino acids. The molecular weight was 2.29 kDa and the isoelectric point was 9.28. The alignment showed that AcPI had a high identity with CsPIC2 (78.6%, AoPI (77.4%, OrcPI (75.7% and HPI2 (72.4%. Quantitative real-time polymerase chain reaction (qRT-PCR analyses in different tissues showed that the expression pattern of AcPI was different from the B-class genes in eudicots. AcPI was expressed in all the tissues investigated. The expression level was very low in fruit stems, bracts, leaves and sepals, high in petals and carpels, and moderate in apical meristems, flesh and stamens. The qRT-PCR analyses in different stages indicated that the expression of AcPI reached the highest level at 40 days after flower inducement, when the multiple fruit and floral organs were forming. It proved the important role of AcPI in floral organs and fruit development. The 35S::AcPI transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants.

  12. CONVERSION OF PINEAPPLE JUICE WASTE INTO LACTIC ACID IN BATCH AND FED – BATCH FERMENTATION SYSTEMS

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    Abdullah Mochamad Busairi

    2012-01-01

    Full Text Available Pineapple juice waste contains valuable components, which are mainly sucrose, glucose, and fructose. Recently, lactic acid has been considered to be an important raw material for the production of biodegradable lactide polymer. The fermentation experiments were carried out in a 3 litres fermentor (Biostat B Model under anaerobic condition with stirring speed of 50 rpm, temperature at 40oC, and pH of 6.00. Effect of feed concentration on lactic acid production, bacterial growth, substrate utilisation and productivity was studied. The results obtained from fed- batch culture fermentation showed that the maximum lactic acid productivity was 0.44 g/L.h for feed concentration of 90 g/L at 48 hours. Whereas the lactic acid productivity obtained from fed-batch culture was twice and half fold higher than that of batch culture productivity.  Buangan jus nanas mengandung komponen yang berharga terutama sukrosa, glukosa, dan fruktosa. Asam laktat adalah bahan baku yang terbaru dan penting untuk dibuat sebagai polimer laktat yang dapat terdegradasi oleh lingkungan. Percobaan dilakukan pada fermentor 3 liter (Model Biostat B di bawah kondisi anaerob dengan kecepatan pengadukan 50 rpm, temperatur 40oC, dan pH 6,00. Pengaruh konsentrasi umpan terhadap produksi asam laktat, pertumbuhan mikroba, pengggunaan substrat dan produktivitas telah dipelajari. Hasil yang didapatkan pada fermentasi dengan menggunakan sistem fed-batch menunjukkan bahwa produktivitas asam laktat maksimum adalah 0.44 g/L,jam dengan konsentrasi umpan, 90 g/L pada waktu 48 jam. Bahkan produktivitas asam laktat yang didapat pada kultur fed-batch lebih tinggi 2,5 kali dari pada proses menggunakan sistem batch

  13. Ripening-dependent metabolic changes in the volatiles of pineapple (Ananas comosus (L.) Merr.) fruit: I. Characterization of pineapple aroma compounds by comprehensive two-dimensional gas chromatography-mass spectrometry.

    Science.gov (United States)

    Steingass, Christof Björn; Carle, Reinhold; Schmarr, Hans-Georg

    2015-03-01

    Qualitative ripening-dependent changes of pineapple volatiles were studied via headspace solid-phase microextraction and analyzed by comprehensive two-dimensional gas chromatography quadrupole mass spectrometry (HS-SPME-GC×GC-qMS). Early green-ripe stage, post-harvest ripened, and green-ripe fruits at the end of their commercial shelf-life were compared to air-freighted pineapples harvested at full maturity. In total, more than 290 volatiles could be identified by mass spectrometry and their linear retention indices. The majority of compounds comprise esters (methyl and ethyl esters of saturated and unsaturated fatty acids, acetates), terpenes, alcohols, aldehydes, 2-ketones, free fatty acids, and miscellaneous γ- and δ-lactones. The structured separation space obtained by GC×GC allowed revealing various homologous series of compound classes as well as clustering of sesquiterpenes. Post-harvest ripening increased the diversity of the volatile profile compared to both early green-ripe maturity stages and on-plant ripened fruits.

  14. Pineapple by-product and canola oil as partial fat replacers in low-fat beef burger: Effects on oxidative stability, cholesterol content and fatty acid profile.

    Science.gov (United States)

    Selani, Miriam M; Shirado, Giovanna A N; Margiotta, Gregório B; Rasera, Mariana L; Marabesi, Amanda C; Piedade, Sonia M S; Contreras-Castillo, Carmen J; Canniatti-Brazaca, Solange G

    2016-05-01

    The effect of freeze-dried pineapple by-product and canola oil as fat replacers on the oxidative stability, cholesterol content and fatty acid profile of low-fat beef burgers was evaluated. Five treatments were performed: conventional (CN, 20% fat) and four low-fat formulations (10% fat): control (CT), pineapple by-product (PA), canola oil (CO), and pineapple by-product and canola oil (PC). Low-fat cooked burgers showed a mean cholesterol content reduction of 9.15% compared to the CN. Canola oil addition improved the fatty acid profile of the burgers, with increase in the polyunsaturated/saturated fatty acids ratio and decrease in the n-6/n-3 ratio, in the atherogenic and thrombogenic indexes. The oxidative stability of the burgers was affected by the vegetable oil addition. However, at the end of the storage time (120 days), malonaldehyde values of CO and PC were lower than the threshold for the consumer's acceptance. Canola oil, in combination with pineapple by-product, can be considered promising fat replacers in the development of healthier burgers. Copyright © 2016 Elsevier Ltd. All rights reserved.

  15. 16S Ribosomal DNA Characterization of Nitrogen-Fixing Bacteria Isolated from Banana (Musa spp.) and Pineapple (Ananas comosus (L.) Merril)

    Science.gov (United States)

    Magalhães Cruz, Leonardo; Maltempi de Souza, Emanuel; Weber, Olmar Baler; Baldani, José Ivo; Döbereiner, Johanna; de Oliveira Pedrosa, Fábio

    2001-01-01

    Nitrogen-fixing bacteria isolated from banana (Musa spp.) and pineapple (Ananas comosus (L.) Merril) were characterized by amplified 16S ribosomal DNA restriction analysis and 16S rRNA sequence analysis. Herbaspirillum seropedicae, Herbaspirillum rubrisubalbicans, Burkholderia brasilensis, and Burkholderia tropicalis were identified. Eight other types were placed in close proximity to these genera and other alpha and beta Proteobacteria. PMID:11319127

  16. Effects of high-pressure argon and nitrogen treatments on respiration, browning and antioxidant potential of minimally processed pineapples during shelf life.

    Science.gov (United States)

    Wu, Zhi-shuang; Zhang, Min; Wang, Shao-jin

    2012-08-30

    High-pressure (HP) inert gas processing causes inert gas and water molecules to form clathrate hydrates that restrict intracellular water activity and enzymatic reactions. This technique can be used to preserve fruits and vegetables. In this study, minimally processed (MP) pineapples were treated with HP (∼10 MPa) argon (Ar) and nitrogen (N) for 20 min. The effects of these treatments on respiration, browning and antioxidant potential of MP pineapples were investigated after cutting and during 20 days of storage at 4 °C. Lower respiration rate and ethylene production were found in HP Ar- and HP N-treated samples compared with control samples. HP Ar and HP N treatments effectively reduced browning and loss of total phenols and ascorbic acid and maintained antioxidant capacity of MP pineapples. They did not cause a significant decline in tissue firmness or increase in juice leakage. HP Ar treatments had greater effects than HP N treatments on reduction of respiration rate and ethylene production and maintenance of phenolic compounds and DPPH(•) and ABTS(•+) radical-scavenging activities. Both HP Ar and HP N processing had beneficial effects on MP pineapples throughout 20 days of storage at 4 °C. Copyright © 2012 Society of Chemical Industry.

  17. Method by chromatography of gases for the determination of made up of alcoholic fermentation in pineapple fruits (Ananas comosus [L.] Merr)

    International Nuclear Information System (INIS)

    Murillo Williams, A.

    2001-01-01

    The pineapple (Ananas comosus) it is used in the entire world for the fresh consumption or for processed products (canned, frozen, dehydrated). it is cultivated in a wide range of countries and in extreme latitudes.The factors of quality include: maturity, stability, size uniformity, absence of microbial deterioration, absence of burns for the sun, absence of blows, damage for insects and breaking, crowns, color, longitude and integrity. The factors that determine the longevity of a product can be physiologic or pathological. The physiologic conditions refer to the processes of degradation of the fabrics after the crop, while the pathological ones involve the attack of mushrooms and bacteria. In the case of the pineapple, a physiologic problem exists, called black heart, internal brewing (IB), brown endogenic stain ( m ancha cafe endogena , MCE) or chilling injury (CI) that can happen in any part of the world where it is cultivated. This problem has associated to the exhibition from the pineapple to low temperatures, so it is a challenge to manage a fruit like the pineapple that it cannot tolerate low temperatures without problems. In studies made about the physiologic changes that happen during the storage in controlled atmospheres in fruits, it has been observed that the ethanol and the acetaldehyde are volatile compounds associated with metabolic post-crop changes and that they have implication in the quality of the product. (author) [es

  18. In vitro Study of Noni Juice Extract Waste (Morinda citrifolia L.) and Pineapple Industrial Wastes (Ananas comosus L. Merr) as Energy Supplement in Dairy Goat Ration

    Science.gov (United States)

    Evvyernie, D.; Tjakradidjaja, A. S.; Permana, I. G.; Toharmat, T.; Insani, A.

    2018-02-01

    The aim of the study was to evaluate the potency of noni juice extract waste (Morinda citrifolia L.) and pineapple industrial wastes (Ananas comosus L. Merr) as an energy supplement in dairy goat ration through in vitro study. This study used a complete randomized design with 5 treatments and 3 rumen fluid groups. The treatments were R0 as control (60% Napier grass (NG) + 40% concentrate), R1 (45% NG + 15% noni juice extract waste + 40% concentrate) + R2 (45% NG + 15% noni juice extract waste ammoniated + 40% concentrate), R3 (45% NG + 15% pineapple peel + 40% concentrate), and R4 (45% NG + 15% pineapple crown + 40% concentrate). The variables were totalbacterial population, protozoal population, fermentation characteristic (total VFA and NH3 concentration), and digestibility (dry matter and organic matter).Data were analyzed with analysis of variance (ANOVA) and differences among treatments were determined by orthogonal contrast.The results showed that total VFA concentration was significant increased (Ppineapple peel (R3). The average increasing of total VFA concentration was 74% compared to control. As conclusions, 15% pineapple peel or 15% noni juice extract waste can use as an energy supplement by replacing 25% of napier grass in lactating dairy goat ration.

  19. PineElm_SSRdb: a microsatellite marker database identified from genomic, chloroplast, mitochondrial and EST sequences of pineapple (Ananas comosus (L.) Merrill).

    Science.gov (United States)

    Chaudhary, Sakshi; Mishra, Bharat Kumar; Vivek, Thiruvettai; Magadum, Santoshkumar; Yasin, Jeshima Khan

    2016-01-01

    Simple Sequence Repeats or microsatellites are resourceful molecular genetic markers. There are only few reports of SSR identification and development in pineapple. Complete genome sequence of pineapple available in the public domain can be used to develop numerous novel SSRs. Therefore, an attempt was made to identify SSRs from genomic, chloroplast, mitochondrial and EST sequences of pineapple which will help in deciphering genetic makeup of its germplasm resources. A total of 359511 SSRs were identified in pineapple (356385 from genome sequence, 45 from chloroplast sequence, 249 in mitochondrial sequence and 2832 from EST sequences). The list of EST-SSR markers and their details are available in the database. PineElm_SSRdb is an open source database available for non-commercial academic purpose at http://app.bioelm.com/ with a mapping tool which can develop circular maps of selected marker set. This database will be of immense use to breeders, researchers and graduates working on Ananas spp. and to others working on cross-species transferability of markers, investigating diversity, mapping and DNA fingerprinting.

  20. The expression patterns of bromelain and AcCYS1 correlate with blackheart resistance in pineapple fruits submitted to postharvest chilling stress.

    Science.gov (United States)

    Raimbault, Astrid-Kim; Zuily-Fodil, Yasmine; Soler, Alain; Mora, Phillipe; Cruz de Carvalho, Maria H

    2013-11-01

    Blackheart is a physiological disorder induced by postharvest chilling storage during pineapple fruit export shipping. The aim of this study was to check the involvement of bromelain, the cysteine protease protein family abundantly present in pineapple fruits, and AcCYS1, an endogenous inhibitor of bromelain, in the development of blackheart. For this we checked the response to postharvest chilling treatment of two pineapple varieties (MD2 and Smooth Cayenne) differing in their resistance to blackheart. Quantitative RT-PCR analyses showed that postharvest chilling treatment induced a down-regulation of bromelain transcript accumulation in both varieties with the most dramatic drop in the resistant variety. Regarding AcCYS1 transcript accumulation, the varieties showed opposite trends with an up-regulation in the case of the resistant variety and a down-regulation in the susceptible one. Taken together our results suggest that the control of bromelain and AcCYS1 expression levels directly correlates to the resistance to blackheart development in pineapple fruits. Copyright © 2013 Elsevier GmbH. All rights reserved.

  1. Taxonomic structure of the yeasts and lactic acid bacteria microbiota of pineapple (Ananas comosus L. Merr.) and use of autochthonous starters for minimally processing.

    Science.gov (United States)

    Di Cagno, Raffaella; Cardinali, Gainluigi; Minervini, Giovanna; Antonielli, Livio; Rizzello, Carlo Giuseppe; Ricciuti, Patrizia; Gobbetti, Marco

    2010-05-01

    Pichia guilliermondii was the only identified yeast in pineapple fruits. Lactobacillus plantarum and Lactobacillus rossiae were the main identified species of lactic acid bacteria. Typing of lactic acid bacteria differentiated isolates depending on the layers. L. plantarum 1OR12 and L. rossiae 2MR10 were selected within the lactic acid bacteria isolates based on the kinetics of growth and acidification. Five technological options, including minimal processing, were considered for pineapple: heating at 72 degrees C for 15 s (HP); spontaneous fermentation without (FP) or followed by heating (FHP), and fermentation by selected autochthonous L. plantarum 1OR12 and L. rossiae 2MR10 without (SP) or preceded by heating (HSP). After 30 days of storage at 4 degrees C, HSP and SP had a number of lactic acid bacteria 1000 to 1,000,000 times higher than the other processed pineapples. The number of yeasts was the lowest in HSP and SP. The Community Level Catabolic Profiles of processed pineapples indirectly confirmed the capacity of autochthonous starters to dominate during fermentation. HSP and SP also showed the highest antioxidant activity and firmness, the better preservation of the natural colours and were preferred for odour and overall acceptability. Copyright (c) 2009 Elsevier Ltd. All rights reserved.

  2. Survival and expression of acid resistance genes in Shiga toxin-producing Escherichia coli acid adapted in pineapple juice and exposed to synthetic gastric fluid

    Science.gov (United States)

    Aims: The aim of this research was to examine relative transcriptional expression of acid resistance (AR) genes, rpoS, gadA and adiA, in O157:H7 and non-O157 Shiga toxin-producing Escherichia coli (STEC) serotypes after adaptation to pineapple juice (PJ) and subsequently to determine survival with e...

  3. Expression Patterns, Activities and Carbohydrate-Metabolizing Regulation of Sucrose Phosphate Synthase, Sucrose Synthase and Neutral Invertase in Pineapple Fruit during Development and Ripening

    Science.gov (United States)

    Zhang, Xiu-Mei; Wang, Wei; Du, Li-Qing; Xie, Jiang-Hui; Yao, Yan-Li; Sun, Guang-Ming

    2012-01-01

    Differences in carbohydrate contents and metabolizing-enzyme activities were monitored in apical, medial, basal and core sections of pineapple (Ananas comosus cv. Comte de paris) during fruit development and ripening. Fructose and glucose of various sections in nearly equal amounts were the predominant sugars in the fruitlets, and had obvious differences until the fruit matured. The large rise of sucrose/hexose was accompanied by dramatic changes in sucrose phosphate synthase (SPS) and sucrose synthase (SuSy) activities. By contrast, neutral invertase (NI) activity may provide a mechanism to increase fruit sink strength by increasing hexose concentrations. Furthermore, two cDNAs of Ac-sps (accession no. GQ996582) and Ac-ni (accession no. GQ996581) were first isolated from pineapple fruits utilizing conserved amino-acid sequences. Homology alignment reveals that the amino acid sequences contain some conserved function domains. Transcription expression analysis of Ac-sps, Ac-susy and Ac-ni also indicated distinct patterns related to sugar accumulation and composition of pineapple fruits. It suggests that differential expressions of multiple gene families are necessary for sugar metabolism in various parts and developmental stages of pineapple fruit. A cycle of sucrose breakdown in the cytosol of sink tissues could be mediated through both Ac-SuSy and Ac-NI, and Ac-NI could be involved in regulating crucial steps by generating sugar signals to the cells in a temporally and spatially restricted fashion. PMID:22949808

  4. Effects of Nitrogen Supplementation on Yeast (Candida utilis Biomass Production by Using Pineapple (Ananas comosus Waste Extracted Medium

    Directory of Open Access Journals (Sweden)

    Rosma, A.

    2007-01-01

    Full Text Available Pineapple waste medium was used to cultivate yeast, Candida utilis. It served as the sole carbon and energy source for the yeast growth. However, pineapple waste media contain very little nitrogen (0.003-0.015% w/v. Various nitrogen sources were incorporate and their effects on biomass, yield and productivity were studied. Significant (p<0.05 increment on biomass production was observed when nitrogen supplement (commercial yeast extract, peptone, ammonium dihydrogen phosphate, ammonium sulphate and potassium nitrate was added into fermentation medium. Commercial yeast extract, Maxarome® which increased 55.2% of biomass production at 0.09% (w/v nitrogen content, is the most suitable among the selected organic source. On the other hand, ammonium dihydrogen phosphate at 0.09% (w/v nitrogen content is comparable inorganic source which enhanced 53.7% of production. Total nitrogen content of each treatment at 0.05% (w/v showed that nitrogen supplied was not fully utilized as substrate limitation in the fermentation medium.

  5. Comparative genomics analysis of rice and pineapple contributes to understand the chromosome number reduction and genomic changes in grasses

    Directory of Open Access Journals (Sweden)

    Jinpeng Wang

    2016-10-01

    Full Text Available Rice is one of the most researched model plant, and has a genome structure most resembling that of the grass common ancestor after a grass common tetraploidization ~100 million years ago. There has been a standing controversy whether there had been 5 or 7 basic chromosomes, before the tetraploidization, which were tackled but could not be well solved for the lacking of a sequenced and assembled outgroup plant to have a conservative genome structure. Recently, the availability of pineapple genome, which has not been subjected to the grass-common tetraploidization, provides a precious opportunity to solve the above controversy and to research into genome changes of rice and other grasses. Here, we performed a comparative genomics analysis of pineapple and rice, and found solid evidence that grass-common ancestor had 2n =2x =14 basic chromosomes before the tetraploidization and duplicated to 2n = 4x = 28 after the event. Moreover, we proposed that enormous gene missing from duplicated regions in rice should be explained by an allotetraploid produced by prominently divergent parental lines, rather than gene losses after their divergence. This means that genome fractionation might have occurred before the formation of the allotetraploid grass ancestor.

  6. Post-harvest quality model of pineapple guava fruit according to storage and weather conditions of cultivation

    Directory of Open Access Journals (Sweden)

    Alfonso Parra-Coronado

    Full Text Available ABSTRACT The post-harvest quality of pineapple guava fruit is determined by the storage and prevailing weather conditions during growth and development. This study proposes a model for post-harvest fruit quality according to the storage and weather conditions in the pineapple guava growing region. Physiologically ripe fruit were collected during two harvests from two locations within the Department of Cundinamarca (Colombia: Tenjo and San Francisco de Sales. The fruits were stored at 18 ± 1 °C (76 ± 5% relative humidity (RH, over 11 days and at 5 ± 1 °C (87 ± 5% RH, over 31 days, and the quality attributes were evaluated every two days. Models of the most significant physio-chemical quality characteristics of the post-harvest fruit were developed by using the Excel® Solver tool for all data obtained in the two crop periods. The results showed that storage and prevailing weather conditions, which differed according to the altitude of the growing site, had considerable impacts on the physio-chemical characteristics of the fruit throughout the post-harvest ripening process.

  7. Functional Properties of a Cysteine Proteinase from Pineapple Fruit with Improved Resistance to Fungal Pathogens in Arabidopsis thaliana

    Directory of Open Access Journals (Sweden)

    Wei Wang

    2014-02-01

    Full Text Available In plant cells, many cysteine proteinases (CPs are synthesized as precursors in the endoplasmic reticulum, and then are subject to post-translational modifications to form the active mature proteinases. They participate in various cellular and physiological functions. Here, AcCP2, a CP from pineapple fruit (Ananas comosus L. belonging to the C1A subfamily is analyzed based on the molecular modeling and homology alignment. Transcripts of AcCP2 can be detected in the different parts of fruits (particularly outer sarcocarps, and gradually increased during fruit development until maturity. To analyze the substrate specificity of AcCP2, the recombinant protein was overexpressed and purified from Pichia pastoris. The precursor of purified AcCP2 can be processed to a 25 kDa active form after acid treatment (pH 4.3. Its optimum proteolytic activity to Bz-Phe-Val-Arg-NH-Mec is at neutral pH. In addition, the overexpression of AcCP2 gene in Arabidopsis thaliana can improve the resistance to fungal pathogen of Botrytis cinerea. These data indicate that AcCP2 is a multifunctional proteinase, and its expression could cause fruit developmental characteristics of pineapple and resistance responses in transgenic Arabidopsis plants.

  8. Optimization of pineapple pulp residue hydrolysis for lipid production by Rhodotorula glutinis TISTR5159 using as biodiesel feedstock.

    Science.gov (United States)

    Tinoi, Jidapha; Rakariyatham, Nuansri

    2016-08-01

    The higher lipid productivity of Rhodotorula glutinis TISTR5159 was achieved by optimizing the pineapple pulp hydrolysis for releasing the high sugars content. The sequential simplex method operated by varied; solid-to-liquid ratio, sulfuric acid concentration, temperature, and hydrolysis time were successfully applied and the highest sugar content (83.2 g/L) evaluated at a solid-to-liquid ratio of 1:10.8, 3.2% sulfuric acid, 105 °C for 13.9 min. Moreover, the (NH4)2SO4 supplement enhanced the lipid productivity and gave the maximum yields of biomass and lipid of 15.2 g/L and 9.15 g/L (60.2%), respectively. The C16 and C18 fatty acids were found as main components included oleic acid (55.8%), palmitic acid (16.6%), linoleic acid (11.9%), and stearic acid (7.8%). These results present the possibility to convert the sugars in pineapple pulp hydrolysate to lipids. The fatty acid profile was also similar to vegetable oils. Thus, it could be used as potential feedstock for biodiesel production.

  9. In Vivo antiplatelet activity aggregation assay of bromelain fractionate by ethanol from extract pineapple core (Ananas comosus [l.] merr.)

    Science.gov (United States)

    Musfiroh, F. F.; Setiasih, S.; Handayani, S.; Hudiyono, S.; Ilyas, N. M.

    2018-01-01

    Processed fruit from pineapple is one of largest commodities tropical fruit production in Indonesia and will bring the waste from the skin and core. This study aims to isolate bromelain from the pineapple core (Ananas comusus) are purified by fractionation using ethanol and continued by activity test as an antiplatelets agent by in vivo method using white mice male ddy type with acetosal as positive control. Fractionation of crude enzyme bromelain with ethanol produces highest specific activity on ethanol 30-60% fraction (fraction 2) 3.107 Unit/mg and the protein content 61.25 mg with the degree of purity of 155 times compared to crude enzyme. Antiplatelet aggregation tests from in vivo method shows that faction of bromelain with doses 70 μg/KgBW, 140 μg/KgBW, and 210 μg/KgBW can increase a meaningful bleeding time. The highest percentage of increase shown by the isolate at a dose of 210 μg/KgBW in the amount of 515.10 ± 182.23%, when compared to aspirin (231.20 ± 140.66), the value is relatively higher.

  10. Exploring of Agro Waste (Pineapple Leaf, Corn Stalk, and Napier Grass by Chemical Composition and Morphological Study

    Directory of Open Access Journals (Sweden)

    Angzzas Sari Mohd Kassim

    2013-12-01

    Full Text Available Malaysia is a country that is a rich source of agricultural waste material. Three different crops were studied here, including pineapple (Ananas comosus leaf, corn (Zea mays stalk, and Napier grass (Pennisetum purpureum. These crops are characterized as agricultural waste materials in Malaysia and have a high potential to be used as alternative fibers for the paper making industry. The objective of this work was to analyze the chemical composition of pineapple leaf, corn stalk, and Napier grass and to investigate the fiber morphology of these crops. The chemical components analyzed include the following: cellulose (Kurshner-Hoffner method, holocellulose (chlorination method, hemicellulose (chlorination method, ash content (TAPPI method T211-om-93, lignin content (TAPPI method T222-om-98, and soluble sodium hydroxide (TAPPI method T203-om-98. All handsheets morphologies were observed using scanning electron microscopy (SEM. Results indicated each crop has the potential for use as a fiber in paper making. SEM images indicated a condensed composition of the fiber structure. The observed chemical composition and morphology of these three crops indicate their suitability for use as fiber sources for the paper industry.

  11. Qualidade do abacaxi cv Smooth Cayenne minimamente processado Storage of pineapple minimally processed

    Directory of Open Access Journals (Sweden)

    Deborah Santesso Bonnas

    2003-08-01

    Full Text Available O objetivo deste trabalho foi verificar a qualidade e o tempo de conservação de abacaxis minimamente processados, sob armazenamento a 8ºC e 85% U.R., influenciados pelo tipo de corte, o uso ou não de sanificação com hipoclorito de sódio e o tipo de embalagem. Foram analisados os seguintes parâmetros: acidez titulável total, sólidos solúveis totais, pH, aparência, fungos e leveduras, coliformes totais e fecais. Concluiu-se que: a o tipo de corte não influenciou nas características físico-químicas e químicas do abacaxi durante o armazenamento; b o baixo pH, a sanificação e o uso de Boas Práticas de Fabricação inibiram o crescimento de bactérias do grupo coliforme; c a sanificação com hipoclorito não foi eficiente no controle de fungos e leveduras; d os produtos tiveram uma vida útil de prateleira de 6 dias, sob as condições oferecidas.The aim of this work was to evaluate the quality and shelf life of pineapple minimally processed, stored at 8ºC and 85% R.H., influenced by the kind of cutting, sanitization with sodium hipochlorite and packaging. It was analyzed the following parameters: the total titrable acidity, the total soluble solids, pH, the appearance, molds and yeasts, total and fecal coliforms. The conclusions were: a the kind of cutting had not influenced the chemical and physical-chemical characteristics of fruit during storage; b low pH associated with sanitization and the use of good manufacturing practices (GMP's reduced bacterial growth; c the sanitization with sodium hipochlorite was not effective against moulds and yeasts; d the product had a shelf life of 6 days according to the conditions offered.

  12. Effect of gas sparging on flux enhancement and phytochemical properties of clarified pineapple juice by microfiltration

    KAUST Repository

    Laorko, Aporn

    2011-08-01

    Membrane fouling is a major obstacle in the application of microfiltration. Several techniques have been proposed to enhance the permeate flux during microfiltration. Gas sparging is a hydrodynamic method for improving the performance of the membrane process. In this study, a 0.2 μm hollow fiber microfiltration membrane was used to study the effect of cross flow velocity (CFV) and gas injection factor () on the critical and limiting flux during microfiltration of pineapple juice. In addition, the phytochemical properties of clarified juice were investigated. In the absence of gas sparging, the critical and limiting flux increased as the CFV or shear stress number increased. The use of gas sparging led to a remarkable improvement in both the critical and limiting flux but it was more effective at the lower CFV (1.5 m s-1), compared to those at higher CFV (2.0 and 2.5 m s-1). When the gas injection factor was applied at 0.15, 0.25 and 0.35 with a CFV of 1.5 m s -1, the enhancement of 55.6%, 75.5% and 128.2% was achieved for critical flux, while 65.8%, 69.7% and 95.2% was achieved for limiting flux, respectively. The results also indicated that the use of gas sparging was an effective method to reduce reversible fouling and external irreversible fouling rather than internal irreversible fouling. In addition, the CFV and gas sparging did not affect pH, total soluble solids, colour, total phenolic content and the antioxidant property of the clarified juice. The l-ascorbic acid and total vitamin C were significantly decreased when the higher CFV and high gas injection factor were applied. The results also indicated that the use of gas sparging with low CFV was beneficial for flux enhancement while most of the phytochemical properties of the clarified juice was preserved. © 2011 Elsevier B.V. All rights reserved.

  13. Molecular Cloning and Characterization of Four Genes Encoding Ethylene Receptors Associated with Pineapple (Ananas comosus L.) Flowering.

    Science.gov (United States)

    Li, Yun-He; Wu, Qing-Song; Huang, Xia; Liu, Sheng-Hui; Zhang, Hong-Na; Zhang, Zhi; Sun, Guang-Ming

    2016-01-01

    Exogenous ethylene, or ethephon, has been widely used to induce pineapple flowering, but the molecular mechanism behind ethephon induction is still unclear. In this study, we cloned four genes encoding ethylene receptors (designated AcERS1a, AcERS1b, AcETR2a, and AcETR2b). The 5' flanking sequences of these four genes were also cloned by self-formed adaptor PCR and SiteFinding-PCR, and a group of putative cis-acting elements was identified. Phylogenetic tree analysis indicated that AcERS1a, AcERS1b, AcETR2a, and AcETR2b belonged to the plant ERS1s and ETR2/EIN4-like groups. Quantitative real-time PCR showed that AcETR2a and AcETR2b (subfamily 2) were more sensitive to ethylene treatment compared with AcERS1a and AcERS1b (subfamily 1). The relative expression of AcERS1b, AcETR2a, and AcETR2b was significantly increased during the earlier period of pineapple inflorescence formation, especially at 1-9 days after ethylene treatment (DAET), whereas AcERS1a expression changed less than these three genes. In situ hybridization results showed that bract primordia (BP) and flower primordia (FP) appeared at 9 and 21 DAET, respectively, and flowers were formed at 37 DAET. AcERS1a, AcERS1b, AcETR2a, and AcETR2b were mainly expressed in the shoot apex at 1-4 DAET; thereafter, with the appearance of BP and FP, higher expression of these genes was found in these new structures. Finally, at 37 DAET, the expression of these genes was mainly focused in the flower but was also low in other structures. These findings indicate that these four ethylene receptor genes, especially AcERS1b, AcETR2a, and AcETR2b, play important roles during pineapple flowering induced by exogenous ethephon.

  14. De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction

    Science.gov (United States)

    Liu, Chuan-He; Fan, Chao

    2016-01-01

    A remarkable characteristic of pineapple is its ability to undergo floral induction in response to external ethylene stimulation. However, little information is available regarding the molecular mechanism underlying this process. In this study, the differentially expressed genes (DEGs) in plants exposed to 1.80 mL·L−1 (T1) or 2.40 mL·L−1 ethephon (T2) compared with Ct plants (control, cleaning water) were identified using RNA-seq and gene expression profiling. Illumina sequencing generated 65,825,224 high-quality reads that were assembled into 129,594 unigenes with an average sequence length of 1173 bp. Of these unigenes, 24,775 were assigned to specific KEGG pathways, of which metabolic pathways and biosynthesis of secondary metabolites were the most highly represented. Gene Ontology (GO) analysis of the annotated unigenes revealed that the majority were involved in metabolic and cellular processes, cell and cell part, catalytic activity and binding. Gene expression profiling analysis revealed 3788, 3062, and 758 DEGs in the comparisons of T1 with Ct, T2 with Ct, and T2 with T1, respectively. GO analysis indicated that these DEGs were predominantly annotated to metabolic and cellular processes, cell and cell part, catalytic activity, and binding. KEGG pathway analysis revealed the enrichment of several important pathways among the DEGs, including metabolic pathways, biosynthesis of secondary metabolites and plant hormone signal transduction. Thirteen DEGs were identified as candidate genes associated with the process of floral induction by ethephon, including three ERF-like genes, one ETR-like gene, one LTI-like gene, one FT-like gene, one VRN1-like gene, three FRI-like genes, one AP1-like gene, one CAL-like gene, and one AG-like gene. qPCR analysis indicated that the changes in the expression of these 13 candidate genes were consistent with the alterations in the corresponding RPKM values, confirming the accuracy and credibility of the RNA-seq and gene

  15. Influence of irradiation on the degradation speed of pre servants of sliced pineapple preserved by a combined method

    International Nuclear Information System (INIS)

    Garcia A, A.; Castro, D.; Espinosa, R.; Gonzalez, E.; Fernandez, L.; Garcia R, M.O.

    1995-01-01

    In the fulfillment of this study, pineapple of the spanish red variety, cut in 1.0 - 1.5 cm slices, were used. The product was sunken in a saccharose syrup with the addition of sodium methabisulphite and potassium sorbate and packed in polyethylene bags and latter irradiated with 0.2 and KGy doses. The behavior of potassium sorbate with two addition levels is similar during storage at room temperature for the studied irradiation doses. The degradation process of sodium methabisulphite takes place almost totally at one month storage. Unlike of potassium sorbate there exist no meaning differences between sulfur dioxide in irradiated and nonirradiated samples.It is concluded that the irradiation process cause an increasing in the degradation of potassium sorbate basically at the beginning of the storage, while seems no exert some effect on the sodium methabisulphite degradation. (Author)

  16. Efeito da autofecundação em cultivares de abacaxi Effects of self pollination in pineapple cultivars

    Directory of Open Access Journals (Sweden)

    José Renato Santos Cabral

    2003-04-01

    Full Text Available O elevado nível de heterozigose dos parentais utilizados em hibridações é a principal causa da baixa eficiência dos programas de melhoramento genético do abacaxizeiro em gerar novas cultivares. Por outro lado, os efeitos da autofecundação são pouco conhecidos em abacaxi, mas esta estratégia pode proporcionar avanços significativos no melhoramento desta planta. Assim, este trabalho teve como objetivo observar os efeitos da autofecundação em cultivares de abacaxi. Inflorescências das cultivares Primavera, Perolera, Roxo-de-Tefé, Pérola e Smooth Cayenne foram protegidas antes da antese para possibilitar a ocorrência de autofecundação. As sementes produzidas foram germinadas em câmara de crescimento, utilizando-se como substrato do meio de cultura MS, suplementado com 30 g.L-1 de sacarose e solidificado com 2 g.L-1 de "Phytagel. Durante a germinação, observou-se que cerca de 16% das sementes de 'Roxo-de-Tefé' produziram plântulas albinas. Foram obtidas 43 plantas a partir de 'Primavera', cinco de 'Perolera', onze de 'Roxo-de-Tefé' e nenhuma de 'Pérola' e 'Smooth Cayenne'. Todas as plantas da progênie de 'Primavera' apresentaram folhas sem espinhos nos bordos ("piping", evidenciando homozigose para esta característica, enquanto na progênie de 'Perolera' três plantas evidenciaram folhas sem espinhos nos bordos e duas com folhas espinhosas, segregando para o caráter espinescência. Na descendência de 'Roxo-de-Tefé', oito plantas apresentaram folhas de cor roxa e três com folhas verdes, com segregação para a cor da folha, mas todas evidenciaram folhas com espinhos no bordo. As baixas porcentagens de germinação, o crescimento lento e o baixo vigor observados nas plantas em casa de vegetação, viveiro e campo, evidenciaram a ocorrência de depressão por endogamia nestas fases de desenvolvimento.The high level of heterozygosis of parentals used to generate pineapple hybrids has resulted in low efficiency of the

  17. Management and use of dairy cattle feed resources on smallholder certified organic pineapple farms in Central Uganda

    DEFF Research Database (Denmark)

    Kiggundu, Muhammad; Kabi, Fred; Vaarst, Mette

    2014-01-01

    strategies used by farmers to overcome dry season feed shortages on 64 smallholder certified organic pineapple farms. Data was collected using semi-structured questionnaires and two focus group discussions. Majority of households were headed by males (62.9%) while average age of respondents was 42.5 years....... Farmers allocated more land (Pgoats and pigs. Tethering was the commonest cattle management system. Fifty three percent of respondents reported using both natural pastures and crop residues....... Of the respondents, 51.4% conserved feed for their cattle as fodder banks. As a coping strategy to feed shortages, majority (42.9%) of farmer scavenged for feed resources from both organic certified and nonorganic neighbouring farms which is contrary to organic livestock farming standards. It was, therefore...

  18. Nano-Crystalline Diamond Films with Pineapple-Like Morphology Grown by the DC Arcjet vapor Deposition Method

    Science.gov (United States)

    Li, Bin; Zhang, Qin-Jian; Shi, Yan-Chao; Li, Jia-Jun; Li, Hong; Lu, Fan-Xiu; Chen, Guang-Chao

    2014-08-01

    A nano-crystlline diamond film is grown by the dc arcjet chemical vapor deposition method. The film is characterized by scanning electron microscopy, high-resolution transmission electron microscopy (HRTEM), x-ray diffraction (XRD) and Raman spectra, respectively. The nanocrystalline grains are averagely with 80 nm in the size measured by XRD, and further proven by Raman and HRTEM. The observed novel morphology of the growth surface, pineapple-like morphology, is constructed by cubo-octahedral growth zones with a smooth faceted top surface and coarse side surfaces. The as-grown film possesses (100) dominant surface containing a little amorphous sp2 component, which is far different from the nano-crystalline film with the usual cauliflower-like morphology.

  19. Pineapple (Ananas comosus cv. pérola ex vitro growth and mycorrhizal colonization affected by in vitro sucrose concentration

    Directory of Open Access Journals (Sweden)

    Alceu Kunze

    2014-09-01

    Full Text Available The aim of this study was to evaluate the effect of sucrose concentration in the culture medium on growth and on the establishment of mycorrhizas during the acclimatization of pineapple cv. Pérola. The plantlets were micropropagated in MS culture medium with 0, 10, 20 and 30 g L-1 of sucrose and then they were acclimatized during 12 weeks under greenhouse conditions, in a sandy soil - compost mixture, uninoculated or inoculated with a Rhizophagus clarus isolate. Plantlets from the culture medium with 20 g and 30 g of sucrose L-1 showed higher shoot and root biomass than those from sugar-free medium. Mycorrhizal colonization was lower in plantlets micropropagated in sucrose-free medium, but the intensity of arbuscules did not differ among treatments. In the 12-week period of acclimatization, mycorrhizal colonization had no effect on plant biomass.

  20. Expression patterns of flowering genes in leaves of 'Pineapple' sweet orange [Citrus sinensis (L.) Osbeck] and pummelo (Citrus grandis Osbeck).

    Science.gov (United States)

    Pajon, Melanie; Febres, Vicente J; Moore, Gloria A

    2017-08-30

    In citrus the transition from juvenility to mature phase is marked by the capability of a tree to flower and fruit consistently. The long period of juvenility in citrus severely impedes the use of genetic based strategies to improve fruit quality, disease resistance, and responses to abiotic environmental factors. One of the genes whose expression signals flower development in many plant species is FLOWERING LOCUS T (FT). In this study, gene expression levels of flowering genes CiFT1, CiFT2 and CiFT3 were determined using reverse-transcription quantitative real-time PCR in citrus trees over a 1 year period in Florida. Distinct genotypes of citrus trees of different ages were used. In mature trees of pummelo (Citrus grandis Osbeck) and 'Pineapple' sweet orange (Citrus sinensis (L.) Osbeck) the expression of all three CiFT genes was coordinated and significantly higher in April, after flowering was over, regardless of whether they were in the greenhouse or in the field. Interestingly, immature 'Pineapple' seedlings showed significantly high levels of CiFT3 expression in April and June, while CiFT1 and CiFT2 were highest in June, and hence their expression induction was not simultaneous as in mature plants. In mature citrus trees the induction of CiFTs expression in leaves occurs at the end of spring and after flowering has taken place suggesting it is not associated with dormancy interruption and further flower bud development but is probably involved with shoot apex differentiation and flower bud determination. CiFTs were also seasonally induced in immature seedlings, indicating that additional factors must be suppressing flowering induction and their expression has other functions.

  1. Nitric Oxide Mediates the Hormonal Control of Crassulacean Acid Metabolism Expression in Young Pineapple Plants1[W][OA

    Science.gov (United States)

    Freschi, Luciano; Rodrigues, Maria Aurineide; Domingues, Douglas Silva; Purgatto, Eduardo; Van Sluys, Marie-Anne; Magalhaes, Jose Ronaldo; Kaiser, Werner M.; Mercier, Helenice

    2010-01-01

    Genotypic, developmental, and environmental factors converge to determine the degree of Crassulacean acid metabolism (CAM) expression. To characterize the signaling events controlling CAM expression in young pineapple (Ananas comosus) plants, this photosynthetic pathway was modulated through manipulations in water availability. Rapid, intense, and completely reversible up-regulation in CAM expression was triggered by water deficit, as indicated by the rise in nocturnal malate accumulation and in the expression and activity of important CAM enzymes. During both up- and down-regulation of CAM, the degree of CAM expression was positively and negatively correlated with the endogenous levels of abscisic acid (ABA) and cytokinins, respectively. When exogenously applied, ABA stimulated and cytokinins repressed the expression of CAM. However, inhibition of water deficit-induced ABA accumulation did not block the up-regulation of CAM, suggesting that a parallel, non-ABA-dependent signaling route was also operating. Moreover, strong evidence revealed that nitric oxide (NO) may fulfill an important role during CAM signaling. Up-regulation of CAM was clearly observed in NO-treated plants, and a conspicuous temporal and spatial correlation was also evident between NO production and CAM expression. Removal of NO from the tissues either by adding NO scavenger or by inhibiting NO production significantly impaired ABA-induced up-regulation of CAM, indicating that NO likely acts as a key downstream component in the ABA-dependent signaling pathway. Finally, tungstate or glutamine inhibition of the NO-generating enzyme nitrate reductase completely blocked NO production during ABA-induced up-regulation of CAM, characterizing this enzyme as responsible for NO synthesis during CAM signaling in pineapple plants. PMID:20147491

  2. Development and preservation of a cocktail beverage produced from Carrot (Daucus carrota), Pineapple (Ananas comosus) and Mango (Mangifera indoca L,)

    International Nuclear Information System (INIS)

    Boateng, A. A.

    2013-07-01

    Carrot is known to have the potential of providing vital nutrition as well as health and well-being to consumers. In this study, carrot juice was envisaged as a good vehicle for spreading its nutraceutical benefits. The study was then designed in two parts: (a) Survey to establish the beverage consumption pattern in the Anyaa-Sowutuom District in the Greater Accra Region of Ghana and (b) Carrot juice formulation and optimisation. The survey was conducted by administering a questionnaire to volunteer adult consumers. To develop an acceptable carrot juice beverage, pineapple juice and mango juice were included as components to improve taste and flavour respectively. A constrained, simplex centroid mixture design for three components was employed to optimise the proportions of the components in the final product based on sensory attributes. Chemical analyses as well as shelf stability studies were conducted for the optimised beverage preserved by chemicals and gamma radiation. Data obtained from the survey revealed that juices were very popular among consumers. The choice of a particular beverage was dependent on nutrition, taste and colour. Contour plots generated from sensory data of the products were overlaid to determine the optimum ratios of the components of the cocktail beverage. The optimised product consisted of 50-52% Carrot, 18-20% Pineapple and 28-30% Mango. Analysis of the beverage revealed 223 mg/100ml Potassium, 3.92 mg/100ml Pro-vitamin A (Beta carotene) and 43 mg/100ml vitamin C. After 8 weeks of storage 71% Pro-vitamin A was retained even under relatively higher doses (2.5kGy) of gamma radiation. The microbial quality of the beverage was good under all conditions. (au)

  3. The proteolytic system of pineapple stems revisited: Purification and characterization of multiple catalytically active forms.

    Science.gov (United States)

    Matagne, André; Bolle, Laetitia; El Mahyaoui, Rachida; Baeyens-Volant, Danielle; Azarkan, Mohamed

    2017-06-01

    Crude pineapple proteases extract (aka stem bromelain; EC 3.4.22.4) is an important proteolytic mixture that contains enzymes belonging to the cysteine proteases of the papain family. Numerous studies have been reported aiming at the fractionation and characterization of the many molecular species present in the extract, but more efforts are still required to obtain sufficient quantities of the various purified protease forms for detailed physicochemical, enzymatic and structural characterization. In this work, we describe an efficient strategy towards the purification of at least eight enzymatic forms. Thus, following rapid fractionation on a SP-Sepharose FF column, two sub-populations with proteolytic activity were obtained: the unbound (termed acidic) and bound (termed basic) bromelain fractions. Following reversible modification with monomethoxypolyethylene glycol (mPEG), both fractions were further separated on Q-Sepharose FF and SP-Sepharose FF, respectively. This procedure yielded highly purified molecular species, all titrating ca. 1 mol of thiol group per mole of enzyme, with distinct biochemical properties. N-terminal sequencing allowed identifying at least eight forms with proteolytic activity. The basic fraction contained previously identified species, i.e. basic bromelain forms 1 and 2, ananain forms 1 and 2, and comosain (MEROPS identifier: C01.027). Furthermore, a new proteolytic species, showing similarities with basic bomelain forms 1 and 2, was discovered and termed bromelain form 3. The two remaining species were found in the acidic bromelain fraction and were arbitrarily named acidic bromelain forms 1 and 2. Both, acidic bromelain forms 1, 2 and basic bromelain forms 1, 2 and 3 are glycosylated, while ananain forms 1 and 2, and comosain are not. The eight protease forms display different amidase activities against the various substrates tested, namely small synthetic chromogenic compounds (DL-BAPNA and Boc-Ala-Ala-Gly-pNA), fluorogenic compounds

  4. Effect of the ionizing radiation in the sensorial characteristics of the pineapple; Efeitos da radiacao ionizante nas caracteristicas sensoriais do abacaxi

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Josenilda Maria da, E-mail: jmnilda@yahoo.com.b [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil); Spoto, Marta Helena Fillet; Silva, Juliana Pizarro [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil)

    2007-10-15

    The post-harvest quality of the pineapple cultivar Smooth Cayenne was evaluated after the fruit was irradiated with doses of 100 and 150 Gy and stored for 10, 20 and 30 days at 12 deg C ( +-1) and relative humidity of 85% (+- 5). The controls were untreated fruits. Sensorial analyses were made for each storage period to obtain information about the effects of ionizing radiation on the quality of the fruit. The ionizing radiation dosage had little effect on the sensorial characteristics of the pineapple, although the best results were obtained with fruit irradiated with 100 Gy. The 20-day storage period resulted in the highest consumer acceptability, while the 30-day storage period impaired the fruit's external and internal appearance. (author)

  5. Chemical and antioxidant properties of snake tomato (Trichosanthes cucumerina) juice and Pineapple (Ananas comosus) juice blends and their changes during storage.

    Science.gov (United States)

    Bamidele, Oluwaseun P; Fasogbon, Mofoluwaso B

    2017-04-01

    Juice blends made from the mixture of snake tomato (Trichosanthes cucumerina) and Pineapple (Ananas comosus) fruits were analyzed for pH, antioxidant properties, total titratable acidity, vitamin C, lycopene and total phenolic contents after different blend ratios were made. The addition of snake tomato juice increased the vitamin C, total carotene, lycopene and antioxidant properties of the juice blends. The radical scavenging properties of juice blends containing a higher ratio of snake tomato were higher and samples stored at room temperature (29°C) showed an increase in antioxidant properties compared to samples stored at 4°C. In conclusion, snake tomato juice up to 50% may be added to Pineapple juice to make a healthy juice blend. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. The effective use of acai juice, blueberry juice and pineapple juice as negative contrast agents for magnetic resonance cholangiopancreatography in children

    International Nuclear Information System (INIS)

    Bittman, Mark E.; Callahan, Michael J.

    2014-01-01

    Magnetic resonance cholangiopancreatography (MRCP) is commonly performed in the evaluation of known or suspected pancreaticobiliary disease in children. The administration of a negative oral contrast agent can improve the quality of the examination without significant additional cost. We describe our experience with certain brands of acai juice, blueberry juice and pineapple juice as negative oral contrast agents in children. We believe these fruit juices are safe, palatable and may improve MRCP image quality. (orig.)

  7. The effective use of acai juice, blueberry juice and pineapple juice as negative contrast agents for magnetic resonance cholangiopancreatography in children

    Energy Technology Data Exchange (ETDEWEB)

    Bittman, Mark E. [Cohen Children' s Medical Center of New York, North Shore Long Island Jewish Health System, Department of Radiology, New Hyde Park, NY (United States); Callahan, Michael J. [Boston Children' s Hospital, Department of Radiology, Boston, MA (United States)

    2014-07-15

    Magnetic resonance cholangiopancreatography (MRCP) is commonly performed in the evaluation of known or suspected pancreaticobiliary disease in children. The administration of a negative oral contrast agent can improve the quality of the examination without significant additional cost. We describe our experience with certain brands of acai juice, blueberry juice and pineapple juice as negative oral contrast agents in children. We believe these fruit juices are safe, palatable and may improve MRCP image quality. (orig.)

  8. The Use of Alternate Ligno-cellulosic Raw Materials Banana (Musa sapientum) Ankara (Calotropis procera ) and Pineapple (Ananas comosus ) in Handmade Paper & their Blending with Waste Paper.

    OpenAIRE

    Atul Kumar

    2013-01-01

    The studies were made to established suitability of lingo-cellulosic raw materials namely leaf fibreBanana (Musa Sapientum), bast fibre Ankara (Calotropis Procera), & leaf fibre Pineapple (Ananas Comosus) for making pulps for handmade paper industry. This should help in providing a cost effective, good quality cellulosic raw material as an alternate to cost prohibitive traditionally used cotton hosiery waste traditionally used for manufacturing good quality handmade paper & it’s products. Th...

  9. Effect of vital bleaching with solutions containing different concentrations of hydrogen peroxide and pineapple extract as an additive on human enamel using reflectance spectrophotometer: An in vitro study.

    Science.gov (United States)

    Vejai Vekaash, Chitra Janardhanan; Kumar Reddy, Tripuravaram Vinay; Venkatesh, Kondas Vijay

    2017-01-01

    This study aims to evaluate the color change in human enamel bleached with three different concentrations of hydrogen peroxide, containing pineapple extract as an additive in two different timings, using reflectance spectrophotometer. The study aimed to investigate the bleaching efficacy on natural teeth using natural enzymes. Baseline color values of 10 randomly selected artificially stained incisors were obtained. The specimens were divided into three groups of 20 teeth each: Group 1 - 30% hydrogen peroxide, Group II - 20% hydrogen peroxide, and Group III - 10% hydrogen peroxide. One half of the tooth was bleached with hydrogen peroxide, and other was bleached with hydrogen peroxide and pineapple extract for 20 min (Subgroup A) and 10 min (Subgroup B). The results were statistically analyzed using student's t -test. The mean ΔE values of Group IA (31.62 ± 0.9), Group IIA (29.85 ± 1.2), and Group IIIA (28.65 ± 1.2) showed statistically significant higher values when compared to the mean Δ E values of Group 1A (25.02 ± 1.2), Group IIA (22.86 ± 1.1), and Group IIIA (16.56 ± 1.1). Identical results were obtained in Subgroup B. The addition of pineapple extract to hydrogen peroxide resulted in effective bleaching.

  10. Light-induced alterations of pineapple (Ananas comosus [L.] Merr.) juice volatiles during accelerated ageing and mass spectrometric studies into their precursors.

    Science.gov (United States)

    Steingass, Christof Björn; Glock, Mona Pia; Lieb, Veronika Maria; Carle, Reinhold

    2017-10-01

    Alterations of volatiles during accelerated light-induced ageing of pineapple juice were assessed by HS-SPME-GC-MS in a non-targeted profiling analysis over a 16-week period. Multivariate statistics permitted to reveal substantial chemical markers generally describing the effect of light storage. Volatiles generated comprised phenylpropenes, carbonyls, 2-methylthiophene, toluene, and furfural, while concentrations of methyl and ethyl esters, terpenes, and furanones decreased. In addition, the qualitative composition of phenolic compounds and glycoside-bound volatiles in selected samples was characterized by HPLC-DAD-ESI-MS n as well as HR-ESI-MS. The fresh juice contained unique pineapple metabolites such as S-p-coumaryl, S-coniferyl, S-sinapylglutathione, and structurally related derivatives. Among others, the presence of p-coumaroyl, feruloyl, and caffeoylisocitrate as well as three 4-hydroxy-2,5-dimethyl-3(2H)-furanone glycosides in pineapples could be substantiated by the HR-ESI-MS experiment. Mass spectrometric assignments of selected metabolites are presented, and putative linkages between volatiles and their precursors are established. Copyright © 2017 Elsevier Ltd. All rights reserved.

  11. Utilization of composite membrane polyethyleneglycol-polystyrene-cellulose acetate from pineapple leaf fibers in lowering levels of methyl orange batik waste

    Science.gov (United States)

    Delsy, E. V. Y.; Irmanto; Kazanah, F. N.

    2017-02-01

    Pineapple leaves are agricultural waste from the pineapple that the fibers can be utilized as raw material in cellulose acetate membranes. First, made pineapple leaf fibers into pulp and then converted into cellulose acetate by acetylation process in four stages consisting of activation, acetylation, hydrolysis and purification. Cellulose acetate then used as the raw material to manufacture composite membrane with addition of polystyrene and poly (ethylene glycol) as porogen. Composite membrane is made using phase inversion method with dichloromethane-acetone as a solvent. The result of FTIR analysis (Fourier transform infra-red) showed that the absorption of the carbonyl group (C=O) is at 1643.10 cm-1 and acetyl group (C-O ) at 1227.01 cm-1, with a molecular weight of 8.05 x 104 g/mol and the contents (rate) of acetyl is 37.31%. PS-PEG-CA composite membrane had also been characterized by measuring the water flux values and its application to decrease methyl orange content (level) in batik waste. The results showed that the water flux value is of 25.62 L/(m2.hour), and the decrease percentage of methyl orange content in batik waste is 71.53%.

  12. Evaluation of the effects of gamma radiation on the quality of pineapple (Ananas comosus (L.) Meer) cv. Smooth Cayenne minimally processed, storaged on differents temperatures and packages

    International Nuclear Information System (INIS)

    Leite, Daniela Terenzi Stuchi

    2006-01-01

    The present work aimed to verify the effects of gamma radiation (doses until 2 kGy), types of packages and temperatures of storage (5, 8 e 12 deg C) on the physicochemical characteristics, on the microbiological contamination and on the sensorial characteristics of pineapple 'Smooth Cayenne' minimally processed. The fruits were selected, washed; peeled and cutted transverse and the slices cutted were cutted on two or for pieces. The pieces were immersed in chlorinated water (100 mg/L) for 3 minutes, flowing and package, irradiated and stored. According with the results obtained in thi present work it was concluded that bigger the temperature of storage more quickly were the browning of the fruits. The loss of fresh weight of pineapple was bigger in the packages of polystyrene comparing with PET package when both are covered with PVC film and smaller in the PET packages covered with the same material. Doses of gamma radiation until 2 kGy did not change the physico-chemical and sensorial characteristics of pineapple 'Smooth Cayenne' minimally processed. The microbiological growth on the pieces of the processed fruit was smaller on the biggest dose. All the samples were is in good conditions for the human consume. (author)

  13. Utilization of pineapple stem juice to enhance enzyme-hydrolytic efficiency for sugarcane bagasse after an optimized pre-treatment with alkaline peroxide

    Energy Technology Data Exchange (ETDEWEB)

    Monte, J.R.; Brienzo, M.; Milagres, A.M.F. [Department of Biotechnology, School of Engineering of Lorena, University of Sao Paulo - USP Estrada Municipal do Campinho, s/no - CP 116, 12602-810 Lorena, SP (Brazil)

    2011-01-15

    The enzymatic hydrolysis of sugarcane bagasse was investigated by treating a peroxide-alkaline bagasse with a pineapple stem juice, xylanase and cellulase. Pre-treatment procedures of sugarcane bagasse with alkaline hydrogen peroxide were evaluated and compared. Analyses were performed using 2{sup 4} factorial designs, with pre-treatment time, temperature, magnesium sulfate and hydrogen peroxide concentration as factors. The responses evaluated were the yield of cellobiose and glucose released from pretreated bagasse after enzymatic hydrolysis. The results show that the highest enzymatic conversion was obtained for bagasse using 2% hydrogen peroxide at 60 C for 16 h in the presence of 0.5% magnesium sulfate. Bagasse (5%) was treated with pineapple stem extract, which contains mixtures of protease and esterase, in combination with xylanase and cellulase. It was observed that the amount of glucose and cellobiose released from bagasse increased with the mixture of enzymes. It is believed that the enzymes present in pineapple extracts are capable of hydrolyze specific linkages that would facilitate the action of digesting plant cell walls enzymes. This increases the amount of glucose and other hexoses that are released during the enzymatic treatment and also reduces the amount of cellulase necessary in a typical hydrolysis. (author)

  14. Characterization of Mexican coriander (Eryngium foetidum) essential oil and its inactivation of Listeria monocytogenes in vitro and during mild thermal pasteurization of pineapple juice.

    Science.gov (United States)

    Ngang, Jean J Essia; Nyegue, Maximilienne A; Ndoye, Foe C; Tchuenchieu Kamgain, Alex D; Sado Kamdem, Sylvain L; Lanciotti, Rosalba; Gardini, Fausto; Etoa, François-Xavier

    2014-03-01

    The aim of this work was to characterize the essential oil (EO) of Eryngium foetidum (EfEO) and assess its activity toward Listeria monocytogenes in broth and during thermal inactivation of the pathogen in pineapple juice. In this respect, EfEO was chemically characterized, and its antilisteria potential in broth as a function of pH, cell load, and EfEO concentration was assessed through a central composite design. Furthermore, the inactivation kinetics of L. monocytogenes in the juice were assessed by combining EfEO and low pasteurization temperatures. A total of 81 compounds were identified from EfEO. The reduction of pH and cell load increased EO activity. The use of only 15 ppm of EfEO during pasteurization of pineapple juice at 60°C reduced the time required for a 4-log reduction in L. monocytogenes CFU/ml by 74.9% (i.e., from 8.5 to 2.1 min) compared with treatment without EfEO. It could be concluded that EfEO activity toward L. monocytogenes increases with the reduction of pH and that it can be used at sublethal concentrations in combination with low temperatures in pineapple juice pasteurization. This study demonstrates that EO-assisted pasteurization is a promising strategy for the reduction of thermal impact during juice production. EfEO is easily available and compatible with many juices and is thus promising for industrial application.

  15. Effect of refrigerated storage on probiotic viability and the production and stability of antimutagenic and antioxidant peptides in yogurt supplemented with pineapple peel.

    Science.gov (United States)

    Sah, B N P; Vasiljevic, T; McKechnie, S; Donkor, O N

    2015-09-01

    Fruit by-products are good resources of carbohydrates, proteins, vitamins, and minerals, which may function as growth nutrients for probiotic bacteria. This research aimed at evaluating effects of pineapple peel powder addition on the viability and activity of Lactobacillus acidophilus (ATCC 4356), Lactobacillus casei (ATCC393), and Lactobacillus paracasei ssp. paracasei (ATCC BAA52) in yogurts throughout storage at 4°C for 28d. Plain and probiotic yogurts supplemented with or without pineapple peel powder or inulin were prepared. The probiotic counts in supplemented yogurts at 28d of storage ranged from 7.68 and 8.03 log cfu/g, one log cycle higher compared with nonsupplemented control yogurt. Degree of proteolysis in synbiotic yogurts was significantly higher than plain yogurts and increased substantially during storage. Crude water-soluble peptide extract of the probiotic yogurt with peel possessed stronger antimutagenic and antioxidant activities [evaluated measuring reducing power and scavenging capacity of 1,1-diphenyl-2-picrylhydrazyl; 2,2'-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid), and hydroxyl radicals] than control and maintained during storage. Pineapple peel, a by-product of juice production, could be proposed as a prebiotic ingredient in the manufacture of yogurts with enhanced nutrition, and functionality. Copyright © 2015 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

  16. Quantification of allyl hexanoate in pineapple beverages and yogurts as a case study to characterise a source of uncertainty in dietary exposure assessment to flavouring substances.

    Science.gov (United States)

    Raffo, A; D'Aloise, A; Magrì, A D; Leclercq, C

    2012-01-01

    One source of uncertainty in the estimation of dietary exposure to flavouring substances is the uncertainty in the occurrence and concentration levels of these substances naturally present or added to foodstuffs. The aim of this study was to assess the variability of concentration levels of allyl hexanoate, considered as a case study, in two main food categories to which it is often added: pineapple juice-based beverages and yogurts containing pineapple. Thirty-four beverages and 29 yogurts, with pineapple fruit or juice and added flavourings declared as ingredients on the package, were purchased from the local market (in Rome) and analysed. Analytical methods based on the stir bar sorptive extraction (SBSE) technique for the isolation of the target analyte, and on GC-MS analysis for final determination, were developed for the two food categories. In beverages, allyl hexanoate concentrations ranged from less than 0.01 to 16.71 mg l(-1), whereas in yogurts they ranged from 0.02 to 89.41 mg kg(-1). Average concentrations in beverages and yogurts with pineapple as the main fruit ingredient (1.91 mg l(-1) for beverages, 9.61 mg kg(-1) for yogurts) were in fair agreement with average use level data reported from industry surveys for the relevant food categories (4.5 and 6.0 mg kg(-1), respectively). Within the group of yogurts a single product was found to contain a level of allyl hexanoate more than 10-fold higher than the average reported use level. The screening techniques developed by the European Food Safety Authority (EFSA) using use level data provided by industry gave estimates of exposure that were of the same order of magnitude as the estimates obtained for regular consumers who would be loyal to the pineapple yogurt and beverage products containing the highest observed concentration of the substance of interest. In this specific case the uncertainty in the results obtained with the use of standard screening techniques for exposure assessment based on industry

  17. FOLIAR FERTILIZATION ON PINEAPPLE QUALITY AND YIELD ADUBAÇÃO FOLIAR NA QUALIDADE E PRODUTIVIDADE DE ABACAXI

    Directory of Open Access Journals (Sweden)

    Marcelo Carvalho Minhoto Teixeira Filho

    2011-04-01

    Full Text Available There are just a few studies using foliar sprays with micronutrients on pineapple crops. The objective of this study was to evaluate the B and Zn effect, as chelate, acid or salt, via foliar feeding, on fruit yield and quality. The experiment was carried out in Guaraçaí, São Paulo State, Brazil, in a loamy medium texture soil, by using Smooth Cayenne (Hawaiian pineapple seedlings. A randomized block design with four replications was adopted, with 110 g ha-1 of B and 250 g ha-1 of Zn for each application. Two foliar sprays were applied, at 7 and 9 months after planting. The B and Zn sources did not affect the total soluble solids contents, titratable acidity, average fruit diameter, fruit length without crown, and maturity index. Only the B, Zn, and K concentrations in the leaves were influenced by the application of micronutrients.

    Poucos são os estudos desenvolvidos com a aplicação via foliar de micronutrientes, na cultura do abacaxi. Este trabalho teve como objetivo avaliar os efeitos de B e Zn, em forma de quelato, ácido ou sal, via foliar, buscando-se obter respostas sobre os efeitos na produtividade e qualidade dos frutos. O experimento foi realizado em Guaraçaí (SP, em solo com textura média. Foram utilizadas mudas tipo filhote, da cultivar Smooth Cayenne (Havaiano. O delineamento experimental adotado foi o de blocos ao acaso, com quatro repetições, utilizando-se fontes para fornecer, em cada aplicação, 110 g ha-1 de B e 250 g ha-1 de Zn. Foram realizadas duas pulverizações foliares, aos 7 e 9 meses após o plantio. As fontes de B e Zn não exerceram efeito nos teores de sólidos solúveis totais, acidez titulável, diâmetro médio do fruto, comprimento do fruto sem coroa e índice de maturação. Apenas os teores de B, Zn e K, na

  18. Irradiation on cereal bars incorporated with pineapple skin; Irradiacao em barras de cereais incorporadas com casca de abacaxi

    Energy Technology Data Exchange (ETDEWEB)

    Souza, Adriana Regia Marques; Silva, Yasmini Portes Abraham; Costa, Naiane Vieira; Almeida, Thatyana Lacerda; Damiani, Clarissa, E-mail: drilavras@yahoo.com.br, E-mail: yasmibipas@hotmail.com, E-mail: naiane@hotmail.com, E-mail: thototy@gmail.com, E-mail: damianiclarissa@hotmail.com [Universidade Federal de Goias, Escola de Agronomia e Engenharia de Alimentos, Goiania, GO (Brazil); Arthur, Valter, E-mail: arthur@cena.usp.br [Centro de Energia Nuclear na Agricultura, Laboratorio de Radioentomologia e Irradiacao de Alimentos, Piracicaba, SP (Brazil); Lage, Moacir Evandro, E-mail: moacir@cpa.vet.ufg.br [Universidade Federal de Goias, Escola de Veterinaria, Goiania, GO (Brazil); Asquieri, Eduardo Ramirez, E-mail: asquieri@gmail.com [Universidade Federal de Goias, Escola de Farmacia, Goiania, GO (Brazil)

    2011-10-15

    One of the major current concerns to the food industry is the management of residues generated in its production processes. Thus, several studies have been developed, seeking alternative uses for these residues, in order to minimize environmental impacts and add value to products previously discarded. Combining this idea with the increasingly search for healthy and practical products, by consumers, this study aimed the characterization of cereal bars irradiated with doses of 0 kGy, 1 kGy, and 2 kGy, incorporated with dried pineapple skin. The following analyses were carried out: moisture, proteins, lipids, ashes, carbohydrates, energetic value, antioxidant potential, phenolic content, organic acids, and fatty acids profile. The results observed for the centesimal composition did not vary as a function of the radiation doses used, reducing only the levels of antioxidants, phenolic compounds, and organic acids. The product showed potential for becoming an effective way of reusing a food industry residue and the irradiation interfered on the nutritional characteristics of the final product. (author)

  19. EFFECT OF NITROGEN SOURCE AND INITIAL SUGAR CONCENTRATION ON LACTIC ACID FERMENTATION OF PINEAPPLE WASTE USING L.DELBRUECKII

    Directory of Open Access Journals (Sweden)

    Abdullah Moch Busairi

    2012-02-01

    Full Text Available The liquid pineapple waste contains mainly sucrose, glucose, fructose and other nutrients. It therefore can potentially be used as carbon source for lactic acid fermentation. The lactic acid is utilised in food technology as pH regulator, microbial preservative, buffering agent and in the chemical industry. Recently, lactic acid has been considered to be an important raw material for production of biodegradable lactate polymer. The experiments were carried out in batch fermentation at anaerobic condition with stirring speed: 50 rpm, temperature: 40 oC, pH: 6.0, and inoculum size: 5%. Effect of nitrogen source and initial sugar concentration were studied. The effect of nitrogen source on lactic acid production shows that the yeast extract is highest yield , followed by urea , corn steep liquor, malt sprout and ammonium sulphates with the yield of 78.52; 26.68; 19.14; 14.10 and 5.6 %, respectively. The highest yield of initial sugar concentration on lactic acid production obtained was 78.52 % (54.97 g/l at 70 g/l, if the concentration of sugar was increased to 110 g/l , the lactic acid production or yield decrease to51.53 g/l or 54.24%.

  20. Structural Interaction Between GFP-Labeled Diazotrophic Endophytic Bacterium Herbaspirillum seropedicae RAM10 and Pineapple Plantlets 'VitóRia'.

    Science.gov (United States)

    Estrela Borges Baldotto, Lílian; Lopes Olivares, Fábio; Bressan-Smith, Ricardo

    2011-01-01

    The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae.

  1. Leaf margin phenotype-specific restriction-site-associated DNA-derived markers for pineapple (Ananas comosus L.).

    Science.gov (United States)

    Urasaki, Naoya; Goeku, Satoko; Kaneshima, Risa; Takamine, Tomonori; Tarora, Kazuhiko; Takeuchi, Makoto; Moromizato, Chie; Yonamine, Kaname; Hosaka, Fumiko; Terakami, Shingo; Matsumura, Hideo; Yamamoto, Toshiya; Shoda, Moriyuki

    2015-06-01

    To explore genome-wide DNA polymorphisms and identify DNA markers for leaf margin phenotypes, a restriction-site-associated DNA sequencing analysis was employed to analyze three bulked DNAs of F1 progeny from a cross between a 'piping-leaf-type' cultivar, 'Yugafu', and a 'spiny-tip-leaf-type' variety, 'Yonekura'. The parents were both Ananas comosus var. comosus. From the analysis, piping-leaf and spiny-tip-leaf gene-specific restriction-site-associated DNA sequencing tags were obtained and designated as PLSTs and STLSTs, respectively. The five PLSTs and two STSLTs were successfully converted to cleaved amplified polymorphic sequence (CAPS) or simple sequence repeat (SSR) markers using the sequence differences between alleles. Based on the genotyping of the F1 with two SSR and three CAPS markers, the five PLST markers were mapped in the vicinity of the P locus, with the closest marker, PLST1_SSR, being located 1.5 cM from the P locus. The two CAPS markers from STLST1 and STLST3 perfectly assessed the 'spiny-leaf type' as homozygotes of the recessive s allele of the S gene. The recombination value between the S locus and STLST loci was 2.4, and STLSTs were located 2.2 cM from the S locus. SSR and CAPS markers are applicable to marker-assisted selection of leaf margin phenotypes in pineapple breeding.

  2. Stability Test of Partially Purified Bromelain from Pineapple (Ananas comosus (L.) Merr) Core Extract in Artificial Stomach Fluid

    Science.gov (United States)

    Setiasih, S.; Adimas, A. Ch. D.; Dzikria, V.; Hudiyono, S.

    2018-01-01

    This study aimed to isolate and purify bromelain from pineapple core (Ananascomosus (L.) Merr) accompanied by a stability test of its enzyme activity in artificial gastric juice. Purification steps start with fractionation by a precipitation method were carried out stepwise using several concentration of ammonium sulfate salt, followed by dialysis prosess and ion exchange chromatography on DEAE-cellulose column. Each step of purification produced an increasing specific activity in enzyme fraction, starting with crude extract, respectively: 0.276 U/mg; 14.591 U/mg; and 16.05 U/mg. Bromelain fraction with the highest level of purity was obtained in 50-80% ammonium sulphate fraction after dialyzed in the amount of 58.15 times compared to the crude extract. Further purification of the enzyme by DEAE-cellulose column produced bromelain which had a purity level 160-fold compared to crude enzyme. The result of bromelain stability test in artificial stomach juice by milk clotting units assay bromelain fraction have proteolytic activity in clotting milk substrate. Exposing bromelain fraction in artificial stomach juice which gave the highest core bromelain proteolytic activity was achieved at estimated volume of 0.4-0.5 mL. Exposure in a period of reaction time to artificial stomach juice that contained pepsin showed relatively stable proteolytic activity in the first 4 hours.

  3. Structural interaction between GFP-labeled diazotrophic endophytic bacterium Herbaspirillum seropedicae RAM10 and pineapple plantlets 'Vitória'

    Directory of Open Access Journals (Sweden)

    Lílian Estrela Borges Baldotto

    2011-03-01

    Full Text Available The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae.

  4. Advances in life cycle assessment and emergy evaluation with case studies in gold mining and pineapple production

    Science.gov (United States)

    Ingwersen, Wesley W.

    Life cycle assessment (LCA) is an internationally standardized framework for assessing the environmental impacts of products that is rapidly evolving to improve understanding and quantification of how complex product systems depend upon and affect the environment. This dissertation contributes to that evolution through the development of new methods for measuring impacts, estimating the uncertainty of impacts, and measuring ranges of environmental performance, with a focus on product systems in non-OECD countries that have not been well characterized. The integration of a measure of total energy use, emergy, is demonstrated in an LCA of gold from the Yanacocha mine in Peru in the second chapter. A model for estimating the accuracy of emergy results is proposed in the following chapter. The fourth chapter presents a template for LCA-based quantification of the range of environmental performance for tropical agricultural products using the example of fresh pineapple production for export in Costa Rica that can be used to create product labels with environmental information. The final chapter synthesizes how each methodological contribution will together improve the science of measuring product environmental performance.

  5. Effect of sucrose and pectin addition on physical, chemical, thermal and rheological properties of frozen/thawed pineapple pulps

    Science.gov (United States)

    Conceição, Márcia Cavalcante; Fernandes, Tatiana Nunes; Prado, Mônica Elisabeth Torres; de Resende, Jaime Vilela

    2012-09-01

    Pectin (0-1.0 g/100 mL) and sucrose (0-20 g/100 mL) were added to pineapple pulp to improve their rheological properties, thermal properties and stability after freezing and thawing processes. The properties of the mixes were characterized before and after freezing and thawing. Samples were frozen at -20°C, and the freeze concentration was evaluated every 60 min. The thawing rate was evaluated at 19°C and quantified by photographic editing and image analysis software. The thawing rates and values for the freeze concentration were leveled out at pectin concentrations above 0.5 g/100 mL pectin, which indicated that pectin functions to maintain structural homogeneity during freezing. In the thawed samples, the plastic viscosity values were leveled out from pectin concentrations (0.25-0.75 g/100 mL) as the sucrose concentration increased when compared to unfrozen samples. The differences between the rheological parameters of the unfrozen and frozen/thawed pulps, the higher yield stress values after thawing were attributed to the size of suspended particles in the pulp. Applications can specify formulations of frozen products containing pectin, where these properties can be handled after thawing the product.

  6. Performance and carcass quality of broiler chickens fed diet containing pineapple waste meal fermented by “ragi tape”

    Science.gov (United States)

    Mandey, J. S.; Tulung, B.; Leke, J. R.; Sondakh, B. F. J.

    2018-01-01

    The study was conducted to determine the effect of pineapple waste meal fermented by “ragi tape” (FPW) in diets on the performance and carcass quality of broilers. The “ragi tape” was a traditional commercial product of yeast. Five dietary treatments containing 0, 5, 10, 15 and 20% levels of FPW with four replicates were fed to 250 broiler chickens for 42 days in a completely randomized design. Feed and water were provided ad libitum. The variables were performance parameters and carcass quality. Results showed that the performance in finisher, carcass percentage and abdominal fat percentage were significantly affected by dietary treatments. Carcass percentage and abdominal fat percentage were significantly decrease in the proportion of 20% of FPW. However, the carcass percentage in treatments R0 - R4 were still in a good category. The higher the levels of FPW the lower the abdominal fat percentage signed that FPW treatments up to 20% resulted good category of broiler carcass. Income over feed cost and broiler cost analysis in 20% FPW treatment obtained the highest income and the lowest was 0% FPW treatment. It can be concluded that FPW can be fed to broiler chickens at up to 20% level.

  7. In vitro and in vivo evaluation of the activity of pineapple (Ananas comosus) on Haemonchus contortus in Santa Inês sheep.

    Science.gov (United States)

    Domingues, Luciana Ferreira; Giglioti, Rodrigo; Feitosa, Karina Alves; Fantatto, Rafaela Regina; Rabelo, Márcio Dias; de Sena Oliveira, Márcia Cristina; Bechara, Gervásio Henrique; de Oliveira, Gilson Pereira; Barioni Junior, Waldomiro; de Souza Chagas, Ana Carolina

    2013-10-18

    The development of resistance to anthelmintics has prompted research into alternative methods of controlling intestinal nematodes in ruminants. This study aimed to assess the activity of Ananas comosus on Haemonchus contortus in Santa Inês sheep. The aqueous extract of pineapple skin (AEPS), bromelain from pineapple stems (B4882) and residue from pineapple processing was evaluated in in vitro and in vivo tests. The enzymatic activity of substances was analyzed by the azocasein method. The egg hatch test (EHT) and larval development test (LDT) were performed using the Embrapa2010 isolate of H. contortus. In the in vivo test, 36 sheep artificially infected with H. contortus were divided into six groups: G1: 2 g/kg BW of the aqueous extract administered for three days; G2: 2 g/kg BW of the industrial pineapple residue for 60 days; G3: 180 mg/animal of bromelain in a single dose; G4: negative control I; G5: positive control (levamisole phosphate); and G6: negative control II. The eggs per gram (EPG) in the feces were counted till 28 days after treatment. LC₅₀ and LC₉₀ were obtained by the probit procedure, while the in vivo test results were analyzed by GLM. The aqueous extract in the in vitro and in vivo test, the bromelain and industrial residue presented 0.102, 0.157, 1.864 and 0.048 enzyme units/mL, respectively. In the egg hatch test, the LC₅₀ and LC₉₀ were respectively 31 and 81 mg/mL for the aqueous extract and 0.50 and 2 mg/mL for bromelain. In the larval development test, the LC₅₀ and LC₉₀ were respectively 1.7 and 7.3 mg/mL for the aqueous extract and 0.019 and 0.086 mg/mL for bromelain. In the in vivo test, the general efficacies of the treatments in relation to the negative control were 22.6%, 42.2%, 3.65% and 89% for the aqueous extract, industrial pineapple residue, bromelain and positive control respectively. The transformed EPG values were 3.19 ± 0.59, 3.32 ± 0.25, 2.85 ± 0.66, 3.44 ± 0.50, 2.28 ± 0.93 and 2.75 ± 0.94 for

  8. Soluble Sugars as the Carbohydrate Reserve for CAM in Pineapple Leaves : Implications for the Role of Pyrophosphate:6-Phosphofructokinase in Glycolysis.

    Science.gov (United States)

    Carnal, N W; Black, C C

    1989-05-01

    Neutral ethanol-soluble sugar pools serve as carbohydrate reserves for Crassulacean acid metabolism (CAM) in pineapple (Ananas comosus (L.) Merr.) leaves. Levels of neutral soluble sugars and glucans fluctuated reciprocally with concentrations of malic acid. Hexose loss from neutral soluble-sugar pools was sufficient to account for malic acid accumulation with about 95% of the required hexose accounted for by turnover of fructose and glucose pools. Hexose loss from starch or starch plus lower molecular weight glucan pools was insufficient to account for nocturnal accumulation of malic acid. The apparent maximum catalytic capacity of pyrophosphate:6-phosphofructokinase (PPi-PFK) at 15 degrees C was about 16 times higher than the mean maximum rate of glycolysis that occurred to support malic acid accumulation in pineapple leaves at night and 12 times higher than the mean maximum rate of hexose turnover from all carbohydrate pools. The apparent maximum catalytic capacity of ATP-PFK at 15 degrees C was about 70% of the activity required to account for the mean maximal rate of hexose turnover from all carbohydrate pools if turnover were completely via glycolysis, and marginally sufficient to account for mean maximal rates of acidification. Therefore, at low night temperatures conducive to CAM and under subsaturating substrate concentrations, PPi-PFK activity, but not ATP-PFK activity, would be sufficient to support the rate of glycolytic carbohydrate processing required for acid accumulation. These data for pineapple establish that there are at least two types of CAM plants with respect to the nature of the carbohydrate reserve utilized to support nighttime CO(2) accumulation. The data further indicate that the glycolytic carbohydrate processing that supports acidification proceeds in different subcellular compartments in plants utilizing different carbohydrate reserves.

  9. Transformações bioquímicas de abacaxi minimamente processado armazenado sob atmosfera modificada Biochemical modifications of pineapple minimally processed under modified atmosphere

    Directory of Open Access Journals (Sweden)

    Mônica Elisabeth Torres Prado

    2004-12-01

    Full Text Available Realizou-se estudo sobre a influência da atmosfera modificada com diferentes concentrações de gases durante o armazenamento de abacaxi cv. Smooth cayennne minimamente processado, por oito dias, à temperatura de 5ºC e 85% de UR. Foram realizadas análises de açúcares neutros, celulose, hemicelulose e poliuronídeos totais na parede celular. O abacaxi minimamente processado foi acondicionado sob duas Atmosferas Modificadas Ativas, uma com 5% de O2 e 5% de CO2 (AM1,outra com 2% de O2 e 10% de CO2 (AM2,e uma Atmosfera Modificada Passiva (Controle durante 8 dias de armazenamento. O uso de atmosferas modificadas ativas permitiu que o abacaxi minimamente processado sofresse menor degradação da parede celular com menor solubilização das hemiceluloses. Abacaxis minimamente processados e armazenados sob atmosfera modificada obtiveram uma vida de prateleira média de 6 dias, a 5º C.Pineapples minimally processed were, stored eight days (5ºC and 85% RH under passive and active atmosphere (MA. Neutral sugars, cellulose, hemicellulose, and total polyuronide analysis in cell wall were done. Two different active MA were tested: 5% of O2 + 5% of CO2 (MA1 and 2% of O2 + 10% of CO2 (MA2 and one passive MA (Control; during eight days of storage. Pineapples minimally processed stored under active modified atmosphere showed degradation of cell wall and less solubilization of hemicelluloses, besides being more effective in control of ethanol production and formation of off flavours. Pineapples minimally processed stored under modified atmosphere, showed life average of 6 days under refrigeration at 5ºC.

  10. Formulation and evaluation of the use of edible coatings based on gellan chunks of fresh pineapple cut to minimize deterioration of quality attributes and mass loss

    International Nuclear Information System (INIS)

    Saborio Marin, Laura

    2014-01-01

    The result of the use of edible coatings on special based of gellan in various formulations applied on pieces of fresh cut pineapple is evaluated to reduce the degradation of the quality characteristics and mass loss, as a plan to increase the usefulness. The effect of storage time of the fruit before processing (TAP), the application of vacuum pressure and concentration has been studied of the active components (gellan (0,50-1,00%), oil (0,00 -0,20%), glycerol and sorbitol (1,5-2,5%), CaCl 2 (1.0-10,0%)) of the forming solutions of coating. Several parameters are evaluated: the loss of juice, weight and composition of O 2 and CO 2 in the headspace, content of soluble solids (SS), titratable acidity (TA) color, darkening and appearance of pineapple chunks. The effect of forming solutions is measured on the adhesion, uniformity, coverage and determination of the water vapor resistance (RVA) coating and the presence of molds and yeasts during refrigerated storage at 5 degrees Celsius and 80% relative humidity. Another finding was that the loss of juice of the pieces uncoated increased (p≤0,05) as the increase TAP of 1 to 3 weeks, still higher than those of the coated pieces (0,50-0,75% gellan ), which have been minimal, while weight loss has decreased. The application of a vacuum pressure of 0,08 MPa before coating the pieces has favored the juice loss after the first two weeks of storage and reduced weight loss of uncoated pieces along the storage. The formulation comprised of 0,75% gellan, 1,5% glycerol, 0,10% oil and 1,0% of CaCl 2 has been which allows further reduction of juice loss and weight of pineapple chunks stored at 5 degrees Celsius and 80% RH [es

  11. Effect of chemical treatment on thermal properties of fibers from pineapple;Estudo da modificacao quimica nas fibras provenientes da coroa do abacaxi

    Energy Technology Data Exchange (ETDEWEB)

    Fernandes, Rafael I.M.; Mulinari, Daniella R.; Carvalho, Kelly C.C.; Conejo, Luiza dos Santos; Voorwald, Herman J.C.; Cioffi, Maria Odila H., E-mail: rafaelimf@hotmail.co [Universidade Estadual Paulista (UNESP), Guaratingueta, SP (Brazil)

    2009-07-01

    In this work the effect of the chemical modification of natural fibres from of pineapple fibres with alkaline solution was studied. After modification the in nature and modified fibres were analyzed by XRD diffractometry and thermogravimetry with objective to evaluate influence chemical treatment in surface and in the thermal properties fibres. With the obtained results it was possible to verify an increase of 10.4 % in the crystallinity index of fibres beyond the increase around 4.5% in the degradation temperature, what it indicates an increase in the stability thermal of the fibres. (author)

  12. The effect of different fibre volume fraction on mechanical properties of banana/pineapple leaf (PaLF)/glass hybrid composite

    Science.gov (United States)

    Hanafee, Z. M.; Khalina, A.; Norkhairunnisa, M.; Syams, Z. Edi; Liew, K. E.

    2017-09-01

    This paper investigates the effect of fibre volume fraction on mechanical properties of banana-pineapple leaf (PaLF)-glass reinforced epoxy resin under tensile loading. Uniaxial tensile tests were carried out on specimens with different fibre contents (30%, 40%, 50% in weight). The composite specimens consists of 13 different combinations. The effect of hybridisation between synthetic and natural fibre onto tensile properties was determined and the optimum fibre volume fraction was obtained at 50% for both banana and PaLF composites. Additional 1 layer of woven glass fibre increased the tensile strength of banana-PaLF composite up to 85%.

  13. Cinética de secagem do resíduo de abacaxi enriquecido Drying kinetics of enriched pineapple residue

    Directory of Open Access Journals (Sweden)

    Hofsky V. Alexandre

    2013-06-01

    Full Text Available O Brasil é um dos maiores produtores de abacaxi do mundo e o maior da América do Sul. O fruto é a parte comercializável da planta enquanto o restante é formado por caule, folha, casca, coroa e talos, considerado resíduo agrícola e não tem sido devidamente aproveitado, resultando em perdas econômicas. Caracterizar e avaliar a cinética de secagem do resíduo (casca de abacaxi (Ananas comosus L. enriquecido utilizando a levedura Saccharomyces cerevisiae em leito estático, em camada fina de secagem, utilizando-se de planejamento experimental fatorial, foi o objetivo deste estudo. A temperatura (40 a 60 ºC e a velocidade do ar de secagem (0,8 a 1,3 m s-1 são as variáveis do processo. O resíduo enriquecido e caracterizado apresentou alto teor de matéria seca (90,27% e elevado teor de proteína (20,21% em relação ao resíduo in natura (7,61%. A cinética de secagem ocorreu no período de taxa decrescente. Os modelos Page, Henderson & Pabis e Lewis, ajustaram os dados experimentais satisfatoriamente, sendo Page discretamente superior aos demais. Verificou-se influência da variável temperatura apenas para a variável resposta umidade, não sendo observada influência estatisticamente significativa da velocidade do ar de secagem.Brazil is the largest producer of pineapple (Ananas comosus L. in South America and one of the largest in the world. The fruit is the marketable part of the plant, while the rest formed by stem, leaves, peel, crowns and shafts is considered as agricultural residue and is not properly used, resulting in economic loss. The aim of this study was to characterize and to evaluate the drying kinetics of enriched pineapple residue (peel using Saccharomyces cerevisiae yeast in static bed in fine layer, using of experimental factorial planning. The temperature (40 to 60 ºC and the wind speed (0.8 to 1.3 m s-1 were the process variables. The characterized enriched residue exhibited a high dry matter content (90.27% and a

  14. Behavior of a portable solar dryer for pineapple fiber Comportamento de um secador solar para fibras de abacaxi

    Directory of Open Access Journals (Sweden)

    Róger Moya

    2012-12-01

    Full Text Available In Costa Rica, there has been a growing interest to use pineapple fiber from plant, for which current processes need to be improved or new processes need to be developed, with emphasis on drying methods. This work presents the design and evaluation of the behavior of a prototype portable solar dryer in four sites of Costa Rica. The design describes the main parts of the dryer, as well as how they were constructed. The behavior was evaluated according to temperature and relative humidity inside e outside the chamber, and the influence of direct and indirect solar radiation. In order to achieve improved dryer efficiency, a dehumidifier was used to control relative humidity, and a heating system was implemented during the hours of low temperature, low solar radiation and high relative humidity. Based on drying time, final moisture content of fibers of pineapple leaves from plant, and the varying hours when fibers were put to dry, the evaluation of the dryer revealed that the best fiber-drying interval is from 6h00 to 14h00, and after that, it is convenient to inject hot air and use a dehumidifier in the dryer in order to increase its efficiency.Na Costa Rica, o interesse pelo uso da fibra proveniente da folha de abacaxizeiro. Tem almentado e essa atividade precisa de melhoramento ou implementação de novos processos para alcançar maior eficiência, principalmente na secagem da fibra. O presente trabalho apresenta o design e comportamento de uma protótipo portátil de secadora solar em 4 sites geográficos da Costa Rica. Estão descritas as principais partes do secador, bem como a metodologia de construção. O comportamento foi avaliado de acordo com a temperatura e umidade relativa interna e externa da câmara e a influencia da radiação direta e indireta. Com a finalidade de melhorar a eficiência da secadora, foi usado um desumidificador para controlar a umidade relativa e ainda um sistema de aquecimento durante as horas de baixa temperatura

  15. Deficiência de boro na cultura do abacaxi 'Pérola' Boron deficiency in pineapple 'Pérola'

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    Susana Cristine Siebeneichler

    2008-12-01

    Full Text Available Vinte plantas de abacaxi "Pérola" foram cultivadas em vasos de plástico de 10 L, em casa de vegetação, com o objetivo de caracterizar os sintomas visuais de deficiência de B nas plantas e nos frutos. Os vasos continham areia de rio lavada cinco vezes com água comum e depois duas vezes com água desionizada. Dez plantas foram irrigadas com solução completa e, de outras dez, o B foi suprimido da solução cinco meses após o plantio. As plantas apresentaram crescimento vegetativo normal e formaram frutos normais. Após a colheita dos frutos, acompanhou-se a formação das mudas do tipo rebento e rebentão basal, onde se observou que as plantas cultivadas sem B formaram mais mudas do tipo rebento do que as plantas cultivadas com B. Na formação das mudas do tipo rebentão basal, não se observou o efeito da supressão do B. No cultivo da planta-soca e em rebentões basais, cultivados em solução nutritiva sem B, observaram-se sintomas de deficiência de B somente no período reprodutivo. Os sintomas de deficiência de B se caracterizaram por: frutos deformados e menores, com formação de excrescência cortiçosa ou secreção de goma entre os frutilhos, rachaduras entre estes preenchidas com excrescência cortiçosa. As mudas tipo filhote formadas nas plantas-soca apresentaram folhas com falhas na borda e pontas secas.Twenty pineapple "Pérola" plants were cultivated in 10L plastic pots in a greenhouse, with the objective of characterizing the visual symptoms of boron deficiency in the pineapple leaves and fruit. The pots contained river sand that was washed five times with common water and two times with deionized water. Ten plants were irrigated five months after planting with a complete nutrient solution and the other ten, without B in the solution. The plants presented regular vegetative growth and fruit formation. After the harvest of the fruit, the formation of shoot and suckers seedling types were accompanied, where it was

  16. Populations and identification of fungi causing postharvest molds, on pineapple peduncles in two regions in Costa Rica

    Directory of Open Access Journals (Sweden)

    Johanny Castro Chinchilla

    2015-11-01

    Full Text Available Pineapple peduncle mold is an important postharvest problem in Costa Rica and it causes fruit rejection. The objective of this study was to identify and quantify the most important fungi in different postharvest phases. Monthly samplings were performed during one production year in 2 regions of Costa Rica. The main genera of fungi were identified and characterized at the molecular level. The colony forming units (CFU were determined in disinfection water, wax, cooling rooms air and in the peel and peduncle of fruits before (NP and after (P processing with the common postharvest treatments of the farms. Fruits were stored in cooling rooms during 22 days and at the end incidence and severity of peduncle molds were evaluated. During the year, changes in fungi populations were observed in all postharvest phases and in the fruits, with higher populations in wax than in disinfection water. Fungi population and molds were higher in the peduncle of NP fruits as compared with P fruits, coincident with larger mold populations at the end of storage. Fungi recovered in the cooling rooms air could also be a source for peduncle molds development. Penicillium purpureogenum, P. diversum and Penicllium sp., were the main fungi identified, with an in vitro high sporulation rate and growing in the peduncle. Moreover, different commercial practices, such as waxing and cooling, where spores were captured, can enhance the peduncle molds development, so it is considered important the cleaning of cooling rooms, as well as developing mechanisms to avoid accumulation in wax of important populations of microorganisms.

  17. Influence of weight and type of planting material on fruit quality and its heterogeneity in pineapple [Ananas comosus (L.) Merrill].

    Science.gov (United States)

    Fassinou Hotegni, V Nicodème; Lommen, Willemien J M; Agbossou, Euloge K; Struik, Paul C

    2014-01-01

    Cultural practices can affect the quality of pineapple fruits and its variation. The objectives of this study were to investigate (a) effects of weight class and type of planting material on fruit quality, heterogeneity in quality and proportion and yield of fruits meeting European export standards, and (b) the improvement in quality, proportion and yield of fruits meeting export standards when flowering was induced at optimum time. Experiments were conducted in Benin with cvs Sugarloaf (a Perola type) and Smooth Cayenne. In cv. Sugarloaf, experimental factors were weight class of planting material (light, mixed, heavy) and time of flowering induction (farmers', optimum) (Experiment 1). In cv. Smooth Cayenne an additional experimental factor was the type of planting material (hapas, ground suckers, a mixture of the two) (Experiment 2). Fruits from heavy planting material had higher infructescence and fruit weights, longer infructescences, shorter crowns, and smaller crown: infructescence length than fruits from light planting material. The type of planting material in Experiment 2 did not significantly affect fruit quality except crown length: fruits from hapas had shorter crowns than those from ground suckers. Crops from heavy planting material had a higher proportion and yield of fruits meeting export standards than those from other weight classes in Experiment 1 only; also the type of planting material in Experiment 2 did not affect these variates. Heterogeneity in fruit quality was usually not reduced by selecting only light or heavy planting material instead of mixing weights; incidentally the coefficient of variation was significantly reduced in fruits from heavy slips only. Heterogeneity was also not reduced by not mixing hapas and ground suckers. Flowering induction at optimum time increased the proportion and yield of fruits meeting export standards in fruits from light and mixed slip weights and in those from the mixture of heavy hapas plus ground suckers.

  18. Characterization of the third SERK gene in pineapple (Ananas comosus) and analysis of its expression and autophosphorylation activity in vitro

    Science.gov (United States)

    Ma, Jun; He, Yehua; Hu, Zhongyi; Xu, Wentian; Xia, Jingxian; Guo, Cuihong; Lin, Shunquan; Chen, Chengjie; Wu, Chenghou; Zhang, Junli

    2014-01-01

    Two somatic embryogenesis receptor-like kinase genes (identified as AcSERK1 and AcSERK2) have previously been characterized from pineapple (Ananas comosus). In this work, we describe the characterization of a third gene (AcSERK3) in this family. AcSERK3 had all the characteristic domains and shared extensive sequence homology with other plant SERKs. AcSERK3 expression was studied by in situ hybridization and quantitative real-time PCR to analyze its function. Intense in situ hybridization signals were observed only in single competent cells and competent cell clusters; no hybridization signal was detected in the subsequent stages of somatic embryogenesis. AcSERK3 was highly expressed in embryogenic callus compared to other organs, e.g., 20–80 fold more than in anther but similar to that of non-embryogenic callus, which was 20–50 fold that of anther. AcSERK3 expression in root was 80 fold higher than in anther and the highest amongst all organs tested. These results indicate that AcSERK3 plays an important role in callus proliferation and root development. His-tagged AcSERK3 protein was successfully expressed and the luminescence of His6-AcSERK3 protein was only ∼5% of that of inactivated AcSERK3 protein and reaction buffer without protein, and 11.3% of that of an extract of host Escherichia coli pET-30a. This finding confirmed that the AcSERK3 fusion protein had autophosphorylation activity. PMID:25249776

  19. Influence of harvest maturity and fruit logistics on pineapple (Ananas comosus [L.] Merr.) volatiles assessed by headspace solid phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC/MS).

    Science.gov (United States)

    Steingass, Christof B; Grauwet, Tara; Carle, Reinhold

    2014-05-01

    Profiling of volatiles from pineapple fruits was performed at four ripening stages using headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME-GC/MS). In total, 142 volatiles were detected, of which 132 were identified. Multivariate data analysis was carried out to assess the effect of post-harvest storage on volatiles composition of green-ripe sea-freighted pineapple in comparison to air-freighted fruits harvested at full maturity. The latter fruits were characterised by volatiles described as potent odorants in pineapples, such as δ-octalactone, γ-lactones, 1-(E,Z)-3,5-undecatriene and 1,3,5,8-undecatetraene, as well as various methyl esters. In contrast, post-harvest storage of green-ripe sea-freighted fruits resulted in an increased formation of ethyl esters, acetates, acetoxy esters and alcohols, thus allowing the authentication of sea- and air-freighted pineapples, respectively. Particularly, compounds presumably derived from methyl-branched amino acid catabolism were identified in the fruits at later post-harvest stages. In addition, physicochemical traits were determined to characterise the fruit maturity stages. Copyright © 2013 Elsevier Ltd. All rights reserved.

  20. The pineapple AcMADS1 promoter confers high level expression in tomato and arabidopsis flowering and fruiting tissues, but AcMADS1 does not complement the tomato LeMADS-RIN (rin) mutant

    Science.gov (United States)

    A previous EST study identified a MADS box transcription factor coding sequence, AcMADS1, that is strongly induced during non-climacteric pineapple fruit ripening. Phylogenetic analyses place the AcMADS1 protein in the same superclade as LeMADS-RIN, a master regulator of fruit ripening upstream of e...

  1. Evaluation of nutritional and antioxidant properties of the tropical fruits banana, litchi, mango, papaya, passion fruit and pineapple cultivated in Réunion French Island.

    Science.gov (United States)

    Septembre-Malaterre, Axelle; Stanislas, Giovédie; Douraguia, Elisabeth; Gonthier, Marie-Paule

    2016-12-01

    Much attention is paid to the beneficial action of fruits against obesity-related oxidative stress. This study evaluated nutritional and antioxidant properties of banana, litchi, mango, papaya, passion fruit and pineapple from Réunion French Island. Results showed that total amounts of carbohydrates, vitamin C and carotenoids were 7.7-67.3g glucose equivalent, 4.7-84.9mg ascorbic acid equivalent and 26.6-3829.2μg β-carotene equivalent/100g fresh weight, respectively. Polyphenols were detected as the most abundant antioxidants (33.0-286.6mg gallic acid equivalent/100g fresh weight) with the highest content from passion fruit. UPLC-MS analysis led to identify epigallocatechin and quercetin derivatives from banana and litchi, ferulic, sinapic, syringic and gallic acids from pineapple and mango, and piceatannol from passion fruit. Polyphenol-rich extracts protected red blood cells and preadipose cells against oxidative stress. Altogether, these findings highlight nutritional benefits of French tropical fruits and their possible interest to improve antioxidant capacities of the body during obesity. Copyright © 2016 Elsevier Ltd. All rights reserved.

  2. Evaluation of volatile profiles obtained for minimally-processed pineapple fruit samples during storage by headspace-solid phase microextraction gas chromatography-mass spectrometry

    Directory of Open Access Journals (Sweden)

    Francielle Crocetta TURAZZI

    Full Text Available Abstract This paper describes the application of the solid-phase microextraction (SPME technique for the determination and monitoring of the volatile profile of minimally-processed pineapple fruit stored at various temperatures (-12 °C, 4 °C and 25 °C for different periods (1, 4 and 10 days. The SPME fiber coating composed of Car/PDMS presented the best performance. The optimal extraction conditions obtained through a Doehlert design were 60 min at 35 °C. The profiles for the volatile compounds content of the fruit at each stage of storage were determined by gas chromatography-mass spectrometry (GC-MS. The variation in the volatile profile over time was greater when the fruit samples were stored at 25 °C and at -12 °C compared to 4 °C. Thus, according to the volatile profiles associated with the storage conditions evaluated in this study, packaged pineapple retains best its fresh fruit aroma when stored at 4 °C.

  3. Inactivation of Spoilage Yeasts by Mentha spicata L. and M. × villosa Huds. Essential Oils in Cashew, Guava, Mango, and Pineapple Juices

    Directory of Open Access Journals (Sweden)

    Erika T. da Cruz Almeida

    2018-05-01

    Full Text Available This study evaluated the efficacy of the essential oil from Mentha spicata L. (MSEO and M. × villosa Huds. (MVEO to inactivate Candida albicans, C. tropicalis, Pichia anomala and Saccharomyces cerevisiae in Sabouraud dextrose broth and cashew, guava, mango, and pineapple juices during 72 h of refrigerated storage. The effects of the incorporation of an anti-yeast effective dose of MSEO on some physicochemical and sensory characteristics of juices were evaluated. The incorporation of 3.75 μL/mL MSEO or 15 μL/mL MVEO caused a ≥5-log reductions in counts of C. albicans, P. anomala, and S. cerevisiae in Sabouraud dextrose broth. In cashew and guava juices, 1.875 μL/mL MSEO or 15 μL/mL MVEO caused ≥5-log reductions in counts of P. anomala and S. cerevisiae. In pineapple juice, 3.75 μL/mL MSEO caused ≥5-log reductions in counts of P. anomala and S. cerevisiae; 15 μL/mL MVEO caused ≥5-log reductions in counts of S. cerevisiae in mango juice. The incorporation of 1.875 μL/mL MSEO did not affect the physicochemical parameters of juices and did not induce negative impacts to cause their possible sensory rejection. These results show the potential of MSEO and MVEO, primarily MSEO, to comprise strategies to control spoilage yeasts in fruit juices.

  4. Removal lead (Pb and mercury (Hg from juaro fish (Pangasius polyuranodon using citric acid from pineapple extract (Ananas comosus as chelating agent

    Directory of Open Access Journals (Sweden)

    Pra Dian Mariadi

    2017-10-01

    Full Text Available An assessment of dietary risk of heavy metals exposure to human is important since it is the main of exposure. The aim of study to measure the contamination of lead and mercury in juaro fish meat and the effort to reduce contaminations using citric acid from pineapple extract as a chelating agent. Samples was soaked and boiled in citric acid extract at concentration 50%, 75 %, 100 % for, 15 min, 30 min, 45 min at 25 0C, 50 0C and 100 0C. The concentration of lead and mercury in Juaro fish lower than the maximum acceptable lever for Pb and Hg respectively (1mg/kg for Pb, 0,5 mg/Kg for Hg. The result indicated that after soaking and boiling in citric acid solution form pineapple extract at concentration 100 % for 45 min at 100 0C reducing heavy metals Pb from 0,02 mg.Kg-1 to 0,003 mg.Kg-1 and Hg from 0,011 mg.Kg-1 to lower than 0,0001 mg.Kg-1. The increasing of soaking time and boiling temperature, the levels of Pb and Hg in Juaro fish Tissue will decreasing.

  5. Study of technical, economic and environmental feasibility of industrial scale production of nanocellulose obtained from the agroindustrial wastes from pineapple peel (Ananas comosus)

    International Nuclear Information System (INIS)

    Camacho Elizondo, Melissa

    2013-01-01

    Technical, economic and environmental study is realized to determine the feasibility of the industrial production of nanocellulose, from agroindustrial wastes of pineapple (Ananas comosus) market oriented of plastic packaging. The market bibliographical studies (national and international) and real capacities of national institutions have determined the most adequate and competitive method for the production of nanocellulose. The conditions to produce nanocellulose are described from agroindustrial wastes of pineapple in an industrial scale, according with the predominant factors in the plastic market. The equilibrium point, cost and price of nanocellulose produced are analyzed for the national market of plastics. The producing unit implemented is evaluated within the general framework of national and international economy and market to contribute the conditions that may to affect the feasibility and profitability of the project. The technical study has demonstrated to count with the adequate technology for the project execution. The economic study of the project has indicated to be economically profitable, considering the results of the NPV ($ 110 031,73), IRR (46,42%) and MARR (19,19%). The SuperPro Designer program has been used as a tool to corroborate the results in the technical-economic study and these have shown that the project has been feasible [es

  6. RNA interference of endochitinases in the sugarcane endophyte Trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease.

    Directory of Open Access Journals (Sweden)

    Aline S Romão-Dumaresq

    Full Text Available The sugarcane root endophyte Trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. Its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including N-acetyl-ß-D-glucosaminidases, chitobiosidases and endochitinases. We used targeted gene deletion and RNA-dependent gene silencing strategies to disrupt N-acetyl-ß-D-glucosaminidase and endochitinase activities of the fungus, and to determine their roles in the biocontrol of soil-borne plant pathogens. The loss of N-acetyl-ß-D-glucosaminidase activities was dispensable for biocontrol of the plurivorous damping-off pathogens Rhizoctonia solani and Sclerotinia sclerotiorum, and of the sugarcane pathogen Ceratocystis paradoxa, the causal agent of pineapple disease. Similarly, suppression of endochitinase activities had no effect on R. solani and S. sclerotiorum disease control, but had a pronounced effect on the ability of T. virens 223 to control pineapple disease. Our work demonstrates a critical requirement for T. virens 223 endochitinase activity in the biocontrol of C. paradoxa sugarcane disease, but not for general antagonism of other soil pathogens. This may reflect its lifestyle as a sugarcane root endophyte.

  7. Sulfur-containing constituents and one 1H-pyrrole-2-carboxylic acid derivative from pineapple [Ananas comosus (L.) Merr.] fruit.

    Science.gov (United States)

    Zheng, Zong-Ping; Ma, Jinyu; Cheng, Ka-Wing; Chao, Jianfei; Zhu, Qin; Chang, Raymond Chuen-Chung; Zhao, Ming; Lin, Zhi-Xiu; Wang, Mingfu

    2010-12-01

    Two sulfur-containing compounds, (S)-2-amino-5-((R)-1-carboxy-2-((E)-3-(4-hydroxy-3-methoxyphenyl)allylthio)ethyl-amino)-5-oxopentanoic acid (1) and (S)-2-amino-5-((R)-1-(carboxymethylamino)-3-((E)-3-(4-hydroxyphenyl)allylthio)-1-oxopropan-2-ylamino)-5-oxopentanoic acid (2), and one 1H-pyrrole-2-carboxylic acid derivative, 6-(3-(1H-pyrrole-2-carbonyloxy)-2-hydroxypropoxy)-3,4,5-trihydroxy-tetrahydro-2H-pyran-2-carboxylic acid (3), together with eighteen known phenolic compounds, were isolated from the fruits of pineapple. Their structures were elucidated by a combination of spectroscopic analyses. Some of these compounds showed inhibitory activities against tyrosinase. The half maximal inhibitory concentration values of compounds 1, 4, 5, 6, 7 are lower than 1 mM. These compounds may contribute to the well-known anti-browning effect of pineapple juice and be potential skin whitening agents in cosmetic applications. Copyright © 2010 Elsevier Ltd. All rights reserved.

  8. Evaluation of genetic variability in micropropagated propagules of ornamental pineapple [Ananas comosus var. bracteatus (Lindley) Coppens and Leal] using RAPD markers.

    Science.gov (United States)

    Santos, M D M; Buso, G C S; Torres, A C

    2008-10-21

    The objective of the present study was to evaluate the genetic variability in micropropagated plantlets of ornamental pineapple, after the fourth period of subculture. The basal culture medium consisted of MS salts, vitamins, 3% sucrose, liquid formulation, supplemented with 6-benzylaminopurine (BAP) at concentrations of 0.125, 0.25, 0.5, 1.0, and 2.0 mg/L. The addition of BAP influenced the occurrence of genetic variation revealed using random amplified polymorphic DNA (RAPD) markers. Of a total of 520 primers tested, 44 were selected and amplified; 402 monomorphic bands (97.2%) and 18 polymorphic bands (2.8%) resulted among regenerated plantlets. The polymorphic fragments were produced by 12 primers (OPA-01, OPA-20, OPB-01, OPB-19, OPC-19, OPF-13, OPL-17, OPM-13, OPP-16, OPT-07, OPV-19, and OPX-03). Among the primers that identified polymorphism, OPA-01, OPA-20, OPB-19, OPC-19, OPL-17, OPP-16, and OPX-3 each showed, one polymorphic band and OPF-13 amplified a maximum of three bands. In this study, the RAPD technique was effective in showing the occurrence of somaclonal variations that occur during the micropropagation process of ornamental pineapple cultivation in BAP-supplemented medium, and it is possible to detect the presence of genetic variation in early stages of plant development.

  9. LONG-TERM PRECONDITIONING OF PLANTLETS: A PRACTICAL METHOD FOR ENHANCING SURVIVAL OF PINEAPPLE (Ananas comosus Merr.) SHOOT TIPS CRYOPRESERVED USING VITRIFICATION.

    Science.gov (United States)

    Hu, W H; Liu, S F; Liaw, S I

    2015-01-01

    The purpose of this study was to develop an efficient cryopreservation protocol for pineapple (Ananas comosus Merr.) shoot tips. The optimal state of pineapple plantlets was investigated by using sucrose preconditioning to enhance survival after cryostorage. To achieve a suitable state of plantlets before cryopreservation, 0.2 M to 0.4 M sucrose concentrations combined with short- (0-7 days), medium- (15-30 days), and long-term (75-150 days) preconditioning periods were compared. The highest survival (100 %) was achieved using the following procedure: intact plantlets underwent long-term preconditioning with 0.2 M sucrose for 135 days, dissected shoot tips were treated with a loading solution containing 2.0 M glycerol + 0.4 M sucrose for 60 min at 25 degree and the shoot tips were dehydrated in PVS2 for 2h at 0 degree C before being plunged in liquid nitrogen. Rewarming was conducted in a water-bath for 30 s at 40 degree C and PVS2 was replaced with a 1.2 M sucrose solution for 30 min at 25 degree C. The shoot tips were transferred on semisolid medium and left in the dark for 1 week, then in dim light for 3 weeks.

  10. Carbon Dioxide Exchange and Acidity Levels in Detached Pineapple, Ananas comosus (L.), Merr., Leaves during the Day at Various Temperatures, Oxygen and Carbon Dioxide Concentrations 1

    Science.gov (United States)

    Moradshahi, Ali; Vines, H. Max; Black, Clanton C.

    1977-01-01

    The effects of temperature, O2, and CO2 on titratable acid content and on CO2 exchange were measured in detached pineapple (Ananas comosus) leaves during the daily 15-hour light period. Comparative measurements were made in air and in CO2-free air. Increasing the leaf temperature from 20 to 35 C decreased the total CO2 uptake in air and slightly increased the total CO2 released into CO2-free air. Between 25 and 35 C, the activation energy for daily acid loss was near 12 kcal mol−1, but at lower temperatures the activation energy was much greater. Increasing O2 or decreasing the CO2 concentration decreased the total CO2 fixation in air, whereas the total CO2 released in CO2-free air was increased. The total acid content remained constant at 20 C, but it decreased progressively with increasing temperature both in air and in CO2-free air. The total acid content at 30 C remained constant in 2% O2 irrespective of CO2 concentration. The total acid content decreased in 21 and 50% O2 as the CO2 increased from 0 to 300, and 540 μl/l of CO2. The data indicate that photorespiration is present in pineapple. The lack of acid loss in 2% O2 suggests that light deacidification is dependent upon respiration and that higher O2 concentrations are required to saturate deacidification. PMID:16659832

  11. Carbon Dioxide Exchange and Acidity Levels in Detached Pineapple, Ananas comosus (L.), Merr., Leaves during the Day at Various Temperatures, Oxygen and Carbon Dioxide Concentrations.

    Science.gov (United States)

    Moradshahi, A; Vines, H M; Black, C C

    1977-02-01

    The effects of temperature, O(2), and CO(2) on titratable acid content and on CO(2) exchange were measured in detached pineapple (Ananas comosus) leaves during the daily 15-hour light period. Comparative measurements were made in air and in CO(2)-free air. Increasing the leaf temperature from 20 to 35 C decreased the total CO(2) uptake in air and slightly increased the total CO(2) released into CO(2)-free air. Between 25 and 35 C, the activation energy for daily acid loss was near 12 kcal mol(-1), but at lower temperatures the activation energy was much greater.Increasing O(2) or decreasing the CO(2) concentration decreased the total CO(2) fixation in air, whereas the total CO(2) released in CO(2)-free air was increased. The total acid content remained constant at 20 C, but it decreased progressively with increasing temperature both in air and in CO(2)-free air. The total acid content at 30 C remained constant in 2% O(2) irrespective of CO(2) concentration. The total acid content decreased in 21 and 50% O(2) as the CO(2) increased from 0 to 300, and 540 mul/l of CO(2). The data indicate that photorespiration is present in pineapple. The lack of acid loss in 2% O(2) suggests that light deacidification is dependent upon respiration and that higher O(2) concentrations are required to saturate deacidification.

  12. Influence of the Fermentation of Pineapple Wastes with the Use of Methanobacterium Strains Separated in Vietnam on the Production of Biogas from them

    Science.gov (United States)

    Nga, Nguyen Thuy; Trang, Nguyen The

    2015-03-01

    When pineapples are processed, the wastes obtained comprise 30-50% of the raw-material weight. These wastes contain sugar, albumins, lipids, and vitamins that decompose under the action of microorganisms existing in nature and, in so doing, pollute the environment. The use of wastes in plants operating on biogas makes it possible to obtain great economic benefits. It was established that the best conditions for the obtaining biogas from pineapple wastes are realized in the case where Methanobacterium strains are used for increasing the rate of fermentation of these wastes and the wastes have a temperature of 35-45oC, a pH index of 7-7.5 at the initial stage of their fermentation, and a C/N ratio of 30/1. Under these conditions, the MT3, MT5, and MT7 strains of Methanobacterium yield the maximum amounts of biogases containing, respectively, 57.4% of CH 4 and 36.1% of CO 2 , 57.2% of CH 4 and 36.3% of CO 2 , and 57.4% of CH 4 and 36.2% of CO 2 .

  13. The efficacy of Mentha arvensis L. and M. piperita L. essential oils in reducing pathogenic bacteria and maintaining quality characteristics in cashew, guava, mango, and pineapple juices.

    Science.gov (United States)

    de Sousa Guedes, Jossana Pereira; da Costa Medeiros, José Alberto; de Souza E Silva, Richard Sidney; de Sousa, Janaína Maria Batista; da Conceição, Maria Lúcia; de Souza, Evandro Leite

    2016-12-05

    This study evaluated the ability of the essential oil from Mentha arvensis L. (MAEO) and M. piperita L. (MPEO) to induce ≥5-log reductions in counts (CFU/mL) of E. coli, L. monocytogenes, and Salmonella enterica serovar Enteritidis in Brain-Heart Infusion broth (BHIB) and cashew, guava, mango, and pineapple juices during refrigerated storage (4±0.5°C). The effects of the incorporation of these essential oils on some physicochemical and sensory parameters of juices were also evaluated. The incorporation of 5, 2.5, 1.25, or 0.625μL/mL of MAEO in BHIB caused a ≥5-log reduction in counts of E. coli and Salmonella Enteritidis after 24h of storage; but only 5μL/mL was able to cause the same reduction in counts of L.monocytogenes. The incorporation of 10μL/mL of MPEO in BHIB caused a ≥5-log reduction in counts of E. coli, Salmonella Enteritidis, and L. monocytogenes after 24h of storage; smaller reductions were observed in BHIB containing 5, 2.5, and 1.25μL/mL of MPEO. Similar reductions were observed when the MAEO or MPEO was incorporated at the same concentrations in mango juice. The incorporation of MAEO or MPEO at all tested concentrations in cashew, guava, and pineapple juices resulted in a ≥5-log reduction in pathogen counts within 1h. The incorporation of MAEO and MPEO (0.625 and 1.25μL/mL, respectively) in fruit juices did not induce alterations in °Brix, pH, and acidity, but negatively affected the taste, aftertaste, and overall acceptance. The use of MAEO or MPEO at low concentrations could constitute an interesting tool to achieve the required 5-log reduction of pathogenic bacteria in cashew, guava, mango, and pineapple fruit juices. However, new methods combining the use of MAEO or MPEO with other technologies are necessary to reduce their negative impacts on specific sensory properties of these juices. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Evaluation of the effects of gamma radiation on physical and chemical characteristics of pineapple (Annanas Comosus (L.) (Meer) cv. smooth Cayenne minimally processed

    Energy Technology Data Exchange (ETDEWEB)

    Perecin, Thalita N.; Oliveira, Ana Claudia S.; Silva, Lucia C.A.; Costa, Marcia H.N.; Arthur, Valter [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil). Lab. de Radiobiologia e Ambiente], e-mail: arthur@cena.usp.br

    2009-07-01

    This study aimed to evaluate the effects of gamma radiation, polypropylene packaging type and temperature (8 deg C) in the physic-chemical characteristics of pineapple 'Smooth Cayenne' minimally processed. The fruits were selected, washed, peeled, sliced crosswise into four parts and placed in sodium hypochlorite 10 ml/L for three minutes, dried and packaged. Were irradiated in a Cobalt-60 source, type Gammacell -220 (dose rate 0.543 kGy/hour), with doses of 0 (control), 1 and 2 kGy and stored in temperature of 8 deg C. Were analyzed color (L factors, a, b), pH, deg Brix, texture, during 5 days after irradiation. The experiment was entirely at random with 3 replicates for each treatment. For the statistic analysis was used the Tuckey test at 5% level of probability. (author)

  15. The effect of electron beam irradiation on the mechanical properties of pineapple leaf fibre (PALF) reinforced high impact polystyrene (HIPS) composites

    Science.gov (United States)

    Siregar, J. P.; Sapuan, S. M.; Rahman, M. Z. A.; Zaman, H. M. D. K.

    2010-05-01

    The effects of electron beam irradiation on the mechanical properties of pineapple leaf fibre reinforced high impact polystyrene (HIPS) composites were studied. Two types of crosslinking agent that has been used in this study were trimethylolpropane triacrylate (TMPTA) and tripropylene gylcol diacrylate (TPGDA). A 50 wt.% of PALF was blended with HIPS and crosslinking agent using Brabender melt mixer at 165 °C. The composites were then irradiated using a 3 MeV electron beam accelerator with dosage of 0-100 kGy. The tensile strength, tensile modulus, flexural strength, flexural modulus, notched and unnotched impat and hardness of composites were measured and the effects of crosslinking agent were also compared.

  16. Evaluation of the effects of gamma radiation on physical and chemical characteristics of pineapple (Annanas Comosus (L.) (Meer) cv. smooth Cayenne minimally processed

    International Nuclear Information System (INIS)

    Perecin, Thalita N.; Oliveira, Ana Claudia S.; Silva, Lucia C.A.; Costa, Marcia H.N.; Arthur, Valter

    2009-01-01

    This study aimed to evaluate the effects of gamma radiation, polypropylene packaging type and temperature (8 deg C) in the physic-chemical characteristics of pineapple 'Smooth Cayenne' minimally processed. The fruits were selected, washed, peeled, sliced crosswise into four parts and placed in sodium hypochlorite 10 ml/L for three minutes, dried and packaged. Were irradiated in a Cobalt-60 source, type Gammacell -220 (dose rate 0.543 kGy/hour), with doses of 0 (control), 1 and 2 kGy and stored in temperature of 8 deg C. Were analyzed color (L factors, a, b), pH, deg Brix, texture, during 5 days after irradiation. The experiment was entirely at random with 3 replicates for each treatment. For the statistic analysis was used the Tuckey test at 5% level of probability. (author)

  17. The effect of electron beam irradiation on the mechanical properties of pineapple leaf fibre (PALF) reinforced high impact polystyrene (HIPS) composites

    Energy Technology Data Exchange (ETDEWEB)

    Siregar, J P; Sapuan, S M [Department of Mechanical and Manufacturing Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor (Malaysia); Rahman, M Z A [Department of Chemistry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor (Malaysia); Zaman, H M D K, E-mail: januarjasmine@yahoo.com [Radiation Processing Technology Division, Malaysia Nuclear Agency, Bangi 43000 Kajang, Selangor (Malaysia)

    2010-05-15

    The effects of electron beam irradiation on the mechanical properties of pineapple leaf fibre reinforced high impact polystyrene (HIPS) composites were studied. Two types of crosslinking agent that has been used in this study were trimethylolpropane triacrylate (TMPTA) and tripropylene gylcol diacrylate (TPGDA). A 50 wt.% of PALF was blended with HIPS and crosslinking agent using Brabender melt mixer at 165 deg. C. The composites were then irradiated using a 3 MeV electron beam accelerator with dosage of 0-100 kGy. The tensile strength, tensile modulus, flexural strength, flexural modulus, notched and unnotched impat and hardness of composites were measured and the effects of crosslinking agent were also compared.

  18. Physiological and enzymatic analyses of pineapple subjected to ionizing radiation; Analises fisiologicas e enzimaticas em abacaxi submetido a tecnologia de radiacao ionizante

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Josenilda Maria da [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil)]. E-mail: jmnilda@yahoo.com.br; Silva, Juliana Pizarro; Spoto, Marta Helena Fillet [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil)

    2007-07-15

    The physiological and enzymatic post-harvest characteristics of the pineapple cultivar Smooth Cayenne were evaluated after the fruits were gamma-irradiated with doses of 100 and 150 Gy and the fruits were stored for 10, 20 and 30 days at 12 deg C ({+-}1) and relative humidity of 85% ({+-}5). Physiological and enzymatic analyses were made for each storage period to evaluate the alterations resulting from the application of ionizing radiation. Control specimens showed higher values of soluble pectins, total pectins, reducing sugars, sucrose and total sugars and lower values of polyphenyloxidase and polygalacturonase enzyme activities. All the analyses indicated that storage time is a significantly influencing factor. The 100 Gy dosage and 20-day storage period presented the best results from the standpoint of maturation and conservation of the fruits quality. (author)

  19. The effect of electron beam irradiation on the mechanical properties of pineapple leaf fibre (PALF) reinforced high impact polystyrene (HIPS) composites

    International Nuclear Information System (INIS)

    Siregar, J P; Sapuan, S M; Rahman, M Z A; Zaman, H M D K

    2010-01-01

    The effects of electron beam irradiation on the mechanical properties of pineapple leaf fibre reinforced high impact polystyrene (HIPS) composites were studied. Two types of crosslinking agent that has been used in this study were trimethylolpropane triacrylate (TMPTA) and tripropylene gylcol diacrylate (TPGDA). A 50 wt.% of PALF was blended with HIPS and crosslinking agent using Brabender melt mixer at 165 deg. C. The composites were then irradiated using a 3 MeV electron beam accelerator with dosage of 0-100 kGy. The tensile strength, tensile modulus, flexural strength, flexural modulus, notched and unnotched impat and hardness of composites were measured and the effects of crosslinking agent were also compared.

  20. Application of an aqueous two-phase micellar system to extract bromelain from pineapple (Ananas comosus) peel waste and analysis of bromelain stability in cosmetic formulations.

    Science.gov (United States)

    Spir, Lívia Genovez; Ataide, Janaína Artem; De Lencastre Novaes, Letícia Celia; Moriel, Patrícia; Mazzola, Priscila Gava; De Borba Gurpilhares, Daniela; Silveira, Edgar; Pessoa, Adalberto; Tambourgi, Elias Basile

    2015-01-01

    Bromelain is a set of proteolytic enzymes found in pineapple (Ananas comosus) tissues such as stem, fruit and leaves. Because of its proteolytic activity, bromelain has potential applications in the cosmetic, pharmaceutical, and food industries. The present study focused on the recovery of bromelain from pineapple peel by liquid-liquid extraction in aqueous two-phase micellar systems (ATPMS), using Triton X-114 (TX-114) and McIlvaine buffer, in the absence and presence of electrolytes CaCl2 and KI; the cloud points of the generated extraction systems were studied by plotting binodal curves. Based on the cloud points, three temperatures were selected for extraction: 30, 33, and 36°C for systems in the absence of salts; 40, 43, and 46°C in the presence of KI; 24, 27, and 30°C in the presence of CaCl2 . Total protein and enzymatic activities were analyzed to monitor bromelain. Employing the ATPMS chosen for extraction (0.5 M KI with 3% TX-114, at pH 6.0, at 40°C), the bromelain extract stability was assessed after incorporation into three cosmetic bases: an anhydrous gel, a cream, and a cream-gel formulation. The cream-gel formulation presented as the most appropriate base to convey bromelain, and its optimal storage conditions were found to be 4.0 ± 0.5°C. The selected ATPMS enabled the extraction of a biomolecule with high added value from waste lined-up in a cosmetic formulation, allowing for exploration of further cosmetic potential. © 2015 American Institute of Chemical Engineers.

  1. An Extended AE-Rich N-Terminal Trunk in Secreted Pineapple Cystatin Enhances Inhibition of Fruit Bromelain and Is Posttranslationally Removed during Ripening1[W][OA

    Science.gov (United States)

    Neuteboom, Leon W.; Matsumoto, Kristie O.; Christopher, David A.

    2009-01-01

    Phytocystatins are potent inhibitors of cysteine proteases and have been shown to participate in senescence, seed and organ biogenesis, and plant defense. However, phytocystatins are generally poor inhibitors of the cysteine protease, bromelain, of pineapple (Ananas comosus). Here, we demonstrated that pineapple cystatin, AcCYS1, inhibited (>95%) stem and fruit bromelain. AcCYS1 is a unique cystatin in that it contains an extended N-terminal trunk (NTT) of 63 residues rich in alanine and glutamate. A signal peptide preceding the NTT is processed in vitro by microsomal membranes giving rise to a 27-kD species. AcCYS1 mRNA was present in roots and leaves but was most abundant in fruit. Using immunofluorescence and immunoelectron microscopy with an AcCYS1-specific antiserum, AcCYS1 was found in the apoplasm. Immunoblot analysis identified a 27-kD protein in fruit, roots, and leaves and a 15-kD species in mature ripe fruit. Ripe fruit extracts proteolytically removed the NTT of 27-kD AcCYS1 in vitro to produce the 15-kD species. Mass spectrometry analysis was used to map the primary cleavage site immediately after a conserved critical glycine-94. The AE-rich NTT was required to inhibit fruit and stem bromelain (>95%), whereas its removal decreased inhibition to 20% (fruit) and 80% (stem) and increased the dissociation equilibrium constant by 1.8-fold as determined by surface plasmon resonance assays. We propose that proteolytic removal of the NTT results in the decrease of the inhibitory potency of AcCYS1 against fruit bromelain during fruit ripening to increase tissue proteolysis, softening, and degradation. PMID:19648229

  2. Purification and Structural and Kinetic Characterization of the Pyrophosphate:Fructose-6-Phosphate 1-Phosphotransferase from the Crassulacean Acid Metabolism Plant, Pineapple.

    Science.gov (United States)

    Tripodi, KEJ.; Podesta, F. E.

    1997-03-01

    Pyrphosphate-dependent phosphofructokinase (PFP) was purified to electrophoretic homogeneity from illuminated pineapple (Ananas comosus) leaves. The purified enzyme consists of a single subunit of 61.5 kD that is immunologically related to the potato tuber PFP [beta] subunit. The native form of PFP likely consists of a homodimer of 97.2 kD, as determined by gel filtration. PFP's glycolytic activity was strongly dependent on pH, displaying a maximum at pH 7.7 to 7.9. Gluconeogenic activity was relatively constant between pH 6.7 and 8.7. Activation by Fru-2,6-bisphosphate (Fru-2,6-P2) was dependent on assay pH. In the glycolytic direction, it activated about 10-fold at pH 6.7, but only 2-fold at pH 7.7. The gluconeogenic reaction was only weakly affected by Fru-2,6-P2. The true substrates for the PFP forward and reverse reactions were Fru-6-phosphate and Mg-pyrophosphate, and Fru-1,6-P2, orthophosphate, and Mg2+, respectively. The results suggest that pineapple PFP displays regulatory properties consistent with a pH-based regulation of its glycolytic activity, in which a decrease in cytosolic pH caused by nocturnal acidification during Crassulacean acid metabolism, which could curtail its activity, is compensated by a parallel increase in its sensitivity to Fru-2,6-P2. It is also evident that the [beta] subunit alone is sufficient to confer PFP with a high catalytic rate and the regulatory properties associated with activation by Fru-2,6-P2.

  3. An extended AE-rich N-terminal trunk in secreted pineapple cystatin enhances inhibition of fruit bromelain and is posttranslationally removed during ripening.

    Science.gov (United States)

    Neuteboom, Leon W; Matsumoto, Kristie O; Christopher, David A

    2009-10-01

    Phytocystatins are potent inhibitors of cysteine proteases and have been shown to participate in senescence, seed and organ biogenesis, and plant defense. However, phytocystatins are generally poor inhibitors of the cysteine protease, bromelain, of pineapple (Ananas comosus). Here, we demonstrated that pineapple cystatin, AcCYS1, inhibited (>95%) stem and fruit bromelain. AcCYS1 is a unique cystatin in that it contains an extended N-terminal trunk (NTT) of 63 residues rich in alanine and glutamate. A signal peptide preceding the NTT is processed in vitro by microsomal membranes giving rise to a 27-kD species. AcCYS1 mRNA was present in roots and leaves but was most abundant in fruit. Using immunofluorescence and immunoelectron microscopy with an AcCYS1-specific antiserum, AcCYS1 was found in the apoplasm. Immunoblot analysis identified a 27-kD protein in fruit, roots, and leaves and a 15-kD species in mature ripe fruit. Ripe fruit extracts proteolytically removed the NTT of 27-kD AcCYS1 in vitro to produce the 15-kD species. Mass spectrometry analysis was used to map the primary cleavage site immediately after a conserved critical glycine-94. The AE-rich NTT was required to inhibit fruit and stem bromelain (>95%), whereas its removal decreased inhibition to 20% (fruit) and 80% (stem) and increased the dissociation equilibrium constant by 1.8-fold as determined by surface plasmon resonance assays. We propose that proteolytic removal of the NTT results in the decrease of the inhibitory potency of AcCYS1 against fruit bromelain during fruit ripening to increase tissue proteolysis, softening, and degradation.

  4. Determination of Cd and Pb in canned pineapple by atomic absorption spectroscopy using a graphite oven; Determinacion de Cd y Pb en pina enlatada por espectrometria de absorcion atomica utilizando horno de grafito

    Energy Technology Data Exchange (ETDEWEB)

    Linares P, G.; Sanchez P, L.; Benavides M, A.; Acosta L, C. [Instituto Nacional de Investigaciones Nucleares, A.P. 18-1027, 11801 Mexico D.F. (Mexico)

    1997-07-01

    Owing to the food susceptibility to be contaminated it is necessary to realize pursuit studies or frequent monitoring about the content of certain metals which represent health risks by its toxicological effects in the human being and another living organisms. In this work the cadmium and lead concentrations are determined in six national brands of canned pineapple analysing separately sugar sirup and the fruit. (Author)

  5. Variação de caracteres em híbridos de abacaxizeiro obtidos de diferentes cruzamentos Characters variation in pineapple hybrids obtained by different crosses

    Directory of Open Access Journals (Sweden)

    José Renato Santos Cabral

    2009-12-01

    Full Text Available A herança dos caracteres utilizados na seleção de híbridos de abacaxi é de suma importância para o sucesso dos programas de melhoramento genético desta fruteira. o trabalho teve como objetivo analisar as variações observadas nos principais caracteres utilizados na seleção de híbridos de abacaxi obtidos de diferentes cruzamentos. Foi realizada a análise de variância dos caracteres: altura da planta do solo até à base do fruto, comprimento do pedúnculo, peso do fruto sem coroa, peso da coroa, teor de sólidos solúveis e acidez titulável de 446 genótipos avaliados e 213 selecionados. observou-se uma diminuição do peso do fruto nos híbridos selecionados na maioria dos cruzamentos e aumento dos sólidos solúveis em todos os cruzamentos, quando comparados com todos os genótipos avaliados. Constatou-se correlação linear simples positiva significativa entre o peso do fruto e a altura da planta. Foi observada também correlação linear simples significativa, de baixa magnitude, positiva entre o peso do fruto e o peso da coroa, a altura da planta e o peso da coroa, a altura da planta e o teor de sólidos solúveis e negativa entre o teor de sólidos solúveis e ocomprimento do pedúnculo. Verificou-se diferença entre os cruzamentos na seleção de genótipos de abacaxi para os caracteres estudados.The inheritance of traits used for the selection of pineapple hybrids is very important for the success of the genetic breeding program of this fruit crop. The objective of the present work was to analyze the variations observed in main traits used for the selection of pineapple hybrids obtained by different crosses. The analysis of variance for 446 evaluated and 213 selected genotypes was carried out for the following traits: plant height from soil to the base of the fruit, peduncle length, weight of the fruit without the crown, weight of the crown, total soluble solids content and titrable acidity. in general, it was observed that

  6. Leitura SPAD em abacaxizeiro imperial cultivado em deficiência de macronutrientes e de boro Spad reading in imperial pineapple under macronutrientes and boron deficiency

    Directory of Open Access Journals (Sweden)

    Maria José Mota Ramos

    2013-03-01

    Full Text Available O equipamento Minolta SPAD-502 mede a intensidade da coloração verde das folhas e tem sido utilizado na quantificação de clorofilas, caracterizando-se pela rapidez, simplicidade e, principalmente, por possibilitar uma avaliação não destrutiva do tecido foliar. O objetivo deste trabalho foi calibrar a leitura SPAD, correlacionando-a com o diagnóstico das deficiências induzidas de macronutrientes e de boro associando às deficiências ao crescimento vegetativo do abacaxizeiro. O experimento constou de oito tratamentos: Completo, -N, -P, -K, -Ca, -Mg, -S e - B, em blocos casualizados completos, com seis repetições. Foram avaliados o comprimento e a largura da folha "D" (marcada e realizadas leituras com o medidor de clorofila SPAD 502. O uso do método de medida indireta da clorofila é adequado para a avaliação do estado nutricional de N e de crescimento vegetativo do abacaxizeiro 'Imperial'. O valor Spad e a concentração foliar de N no tratamento completo são, respectivamente: 75,7 e 14,8 g kg-1, e no deficiente de N: 36,6 e 9,7g kg-1. Com exceção das deficiências de N e P, os demais tratamentos não afetaram a leitura SPAD.The equipment Minolta SPAD-502 measures the intensity of green color of leaves and has been used in the quantification of chlorophyll, characterized by speed, simplicity, and especially by allowing a non-destructive evaluation of the leaf tissue. The objective of this study was to calibrate the SPAD reading and its correlation with the diagnosis of induced deficiencies of macronutrients and boron deficiencies involving the vegetative growth of the pineapple. The experiment consisted of eight treatments: complete, -N,-P, -K, -Ca, -Mg, -S and -B in randomized complete block with six replicates. It was evaluated the length and the width of the sheet "D" (marked and readings taken with the SPAD 502 chlorophyll meter. The use of the method of indirect measurement of chlorophyll is suitable for assessing the

  7. Estudo da viscosidade aparente das polpas de manga (Keitt e abacaxi (Pérola Study of aparent viscosity of mango (Keitt and pineapple (Pérola pulps

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    Daniela Helena PELEGRINE

    2000-04-01

    Full Text Available Neste trabalho foram determinadas experimentalmente, em reômetro rotacional de placas paralelas PQ45 da marca Haake Rotovisco RV-20, o comportamento reológico das polpas integrais e centrifugadas de manga (variedade Keitt e abacaxi (variedade Pérola à temperatura de 30° C. Aos reogramas foi ajustado o modelo reológico de Mizrahi-Berk (M-B, utilizando as medidas da taxa de deformação e tensão de cisalhamento. Os resultados foram apresentados na forma de razão entre viscosidades aparentes (integral/centrifugada em função da taxa de deformação. Esses resultados mostraram o efeito dos sólidos suspensos sobre o comportamento reológico de cada polpa.In this work, it was determined, experimentally, the rheological behavior of mango (Keitt and pineapple (Perola pulps, whole and centrifuged, in a Haake Rotovisco RV-20 rheometer with parallel plates PQ45, at 30° C. On the rheograms it was adjusted the Mizrahi-Berk (M-B model, using the shear rate and shear stress. The results were presented as a ratio between the apparent viscosities (whole/centrifuged with shear rate. This results showed the relative effect of suspended solids on the rheological behavior of each pulp.

  8. Structural Interaction Between GFP-Labeled Diazotrophic Endophytic Bacterium Herbaspirillum seropedicae RAM10 and Pineapple Plantlets ‘VitóRia’

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    Estrela Borges Baldotto, Lílian; Lopes Olivares, Fábio; Bressan-Smith, Ricardo

    2011-01-01

    The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets ‘Vitória’ were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae. PMID:24031612

  9. The chemopreventive action of bromelain, from pineapple stem (Ananas comosus L.), on colon carcinogenesis is related to antiproliferative and proapoptotic effects.

    Science.gov (United States)

    Romano, Barbara; Fasolino, Ines; Pagano, Ester; Capasso, Raffaele; Pace, Simona; De Rosa, Giuseppe; Milic, Natasa; Orlando, Pierangelo; Izzo, Angelo A; Borrelli, Francesca

    2014-03-01

    Colorectal cancer is an important health problem across the world. Here, we investigated the possible antiproliferative/proapoptotic effects of bromelain (from the pineapple stem Ananas comosus L., family Bromeliaceae) in a human colorectal carcinoma cell line and its potential chemopreventive effect in a murine model of colon cancer. Proliferation and apoptosis were evaluated in human colon adenocarcinoma (Caco-2) cells by the (3) H-thymidine incorporation assay and caspase 3/7 activity measurement, respectively. Extracellular signal-related kinase (ERK) and Akt expression were evaluated by Western blot analysis, reactive oxygen species production by a fluorimetric method. In vivo, bromelain was evaluated using the azoxymethane murine model of colon carcinogenesis. Bromelain reduced cell proliferation and promoted apoptosis in Caco-2 cells. The effect of bromelain was associated to downregulation of pERK1/2/total, ERK, and pAkt/Akt expression as well as to reduction of reactive oxygen species production. In vivo, bromelain reduced the development of aberrant crypt foci, polyps, and tumors induced by azoxymethane. Bromelain exerts antiproliferative and proapoptotic effects in colorectal carcinoma cells and chemopreventive actions in colon carcinogenesis in vivo. Bromelain-containing foods and/or bromelain itself may represent good candidates for colorectal cancer chemoprevention. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. New species of Rhinoleucophenga, a potential predator of pineapple mealybugs Nova espécie de Rhinoleucophenga, potencial predadora da cochonilha-do-abacaxizeiro

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    Mark Paul Culik

    2009-04-01

    Full Text Available The objective of this work was to describe a new species of Rhinoleucophenga (Diptera: Drosophilidae. Rhinoleucophenga capixabensis sp. nov. is described based on specimens collected from pineapple (Ananas comosus var. comosus infested with Dysmicoccus brevipes (Hemiptera: Pseudococcidae in the state of Espírito Santo, Brazil. Distinguishing characters of R. capixabensis sp. nov. include hyaline wings, a strong seta on the second antennal segment, body length of about 2-3 mm, uniformly dark brown scutellum, one pair of strong prescutellar setae, ventral epandrial lobes with about 17-18 teeth, and female cerci with approximately 20 long setae.O objetivo deste trabalho foi descrever uma nova espécie de Rhinoleucophenga (Diptera: Drosophilidae. Rhinoleucophenga capixabensis sp. nov. foi descrita com base em espécimes coletados em associação com Dysmicoccus brevipes (Hemiptera: Pseudococcidae em Ananas comosus var. comosus, no Estado do Espírito Santo, Brasil. As principais características de R. capixabensis sp. nov. incluem asas hialinas, uma seta forte no segundo segmento da antena, comprimento do corpo de aproximadamente 2-3 mm, escutelo uniformemente castanho-escuro, um par robusto de setas prescutelares, lobos epadriais ventrais com aproximadamente 17-18 dentes e cercos da fêmea com aproximadamente 20 setas longas.

  11. Identification of volatiles from pineapple (Ananas comosus L.) pulp by comprehensive two-dimensional gas chromatography and gas chromatography/mass spectrometry.

    Science.gov (United States)

    Pedroso, Marcio P; Ferreira, Ernesto C; Hantao, Leandro W; Bogusz, Stanislau; Augusto, Fabio

    2011-07-01

    Combining qualitative data from the chromatographic structure of 2-D gas chromatography with flame ionization detection (GC×GC-FID) and that from gas chromatography-mass spectrometry (GC/MS) should result in a more accurate assignment of the peak identities than the simple analysis by GC/MS, where coelution of analytes is unavoidable in highly complex samples (rendering spectra unsuitable for qualitative purposes) or for compounds in very low concentrations. Using data from GC×GC-FID combined with GC/MS can reveal coelutions that were not detected by mass spectra deconvolution software. In addition, some compounds can be identified according to the structure of the GC×GC-FID chromatogram. In this article, the volatile fractions of fresh and dehydrated pineapple pulp were evaluated. The extraction of the volatiles was performed by dynamic headspace extraction coupled to solid-phase microextraction (DHS-SPME), a technique appropriate for slurries or solid matrices. Extracted analytes were then analyzed by GC×GC-FID and GC/MS. The results obtained using both techniques were combined to improve compound identifications. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  12. Influence of Soda Pulping Variables on Properties of Pineapple (Ananas comosus Merr. Leaf Pulp and Paper Studied by Face-Centered Composite Experimental Design

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    Jantharat Wutisatwongkul

    2016-01-01

    Full Text Available Face-centered composite design (FCC was used to study the effect of pulping variables: soda concentration (4-5 wt%, temperature (90–130°C, and pulping time (20–60 min on the properties of pineapple leaf pulp and paper employing soda pulping. Studied pulp responses were screened yield and lignin content (kappa number. Paper properties, which include tensile index, burst index, and tear index, were also investigated. Effects of the pulping variables on the properties were statistically analyzed using Minitab 16. The optimum conditions to obtain the maximum tensile index were soda concentration of 4 wt%, pulping temperature of 105°C, and pulping time of 20 min. The predicted optimum conditions provided tensile index, burst index, tear index, screened yield, and kappa number of 44.13 kN·m/kg, 1.76 kPa·m2, 1.68 N·m2/kg, 21.29 wt%, and 28.12, respectively, and were experimentally confirmed.

  13. The Supplementation Effects of Iron and Folic Acid Compared with the Multivitamin and Mineral on Female Workers of Childbearing Age in the Pineapple Agribusiness

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    Yaktiworo Indriani

    2013-06-01

    Full Text Available Female workers of childbearing age (WUS as a major of human resources in many agribusiness exposed to anemia. This study aims to improve the iron status of anemic WUS workers with low hemoglobin (Hb levels, who work in a pineapple agribusiness by iron supplementation. This study was conducted two periods, using a double-blind randomized trial design. Subjects were divided into two treatment groups supplements, namely IF that was given iron + folic acid and MVM that was given multi vitamin and mineral containing 15 different vitamins and minerals including iron and folic acid. The subjects of period-1 were 25 married WUS (IF=13, MVM=12 and of period-2 were 15 single WUS (BF=7, MVM=8. Supplementation performed three times weekly for 10 weeks. After supplementation, the levels of Hb, haematocrit (Hc and serum ferritin of BF-group increased, whereas there were declines in MVM-group. The increase in Hb and Hc in married WUS was higher than the single. However, their Hb was fallen down when supplementation was continued without supervision and getting down when not given the supplements anymore. Supplementation with iron is a must for WUS workers, because they are not able to increase their Hb if only rely on their food.

  14. Das Américas para o Mundo: origem, domesticação e dispersão do abacaxizeiro From the Americas to the World: origin, domestication and dispersion of pineapple

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    Maraisa Crestani

    2010-06-01

    Full Text Available O abacaxi (Ananas comosus (L. Merril, fruto símbolo de regiões tropicais e subtropicais, originário das Américas, foi difundido para todo o mundo, principalmente pelos navegantes europeus, em razão de seu aroma e sabor característicos e exuberante aparência. Batizado como o "Rei das Frutas Coloniais", o abacaxi encontra-se entre as 11 frutas mais produzidas no mundo, sendo cultivada e consumida pelos cinco continentes, e o Brasil destaca-se como maior produtor. Assim, o objetivo desta revisão bibliográfica é abordar a história da cultura do abacaxi, contemplando sua botânica, sua origem e dispersão pelo mundo, seu cenário atual, também aspectos relacionados à conservação de germoplasma e ao melhoramento genético.The pineapple(Ananas comosus (L. Merril, fruit-symbol of tropical and subtropical regions, originated in the Americas, was widespread throughout the world mainly by european navigators because of its aroma and flavor, and lush appearance. Named as "King of Colonial Fruits", pineapple is one of the eleven most produced fruit in the world, growing consumed in every continent, with Brazil as the largest producer. Then, the objective of this literature review is to approach the pineapple history, contemplating its botany, origin and dispersion in the world and its current scenario and issues related to germplasm conservation and breeding.

  15. Sintomas visuais de deficiência de macronutrientes e de boro em abacaxizeiro 'imperial' Visual symptons of macronutrients and boron deficiency in 'imperial' pineapple

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    Maria José Mota Ramos

    2009-03-01

    Full Text Available O objetivo deste trabalho foi caracterizar os sintomas visuais de deficiências de macronutrientes e de boro no abacaxizeiro 'Imperial', e associá-los à diagnose foliar. Os tratamentos: Completo, - N, - P, - K, -Ca, - Mg, - S e - B foram aplicados como soluções nutritivas, em vasos plásticos com 14 kg de areia de praia purificada e uma muda de abacaxi, como unidade experimental. O delineamento foi em blocos casualizados completos, com seis repetições. Os sintomas visuais de deficiência nutricional foram fotografados e descritos durante todo o processo de crescimento e desenvolvimento das plantas. Avaliaram-se, também, as concentrações foliares de N, P, K, Ca, Mg, S e B na folha 'D', aos cinco, sete, nove e 12 meses após o plantio. A deficiência de N causou amarelecimento das folhas da planta e da coroa dos frutos e descoramento da polpa do fruto; a de P, folhas novas vermelho-arroxeadas e frutos com a casca avermelhada; a de K, necrose do ápice das folhas mais velhas e escurecimento da polpa do fruto; a de Mg, necrose na base das folhas da coroa do fruto, e na deficiência de B, frutos com cortiça e rachadura nos frutilhos e entre eles. Apesar dos baixos teores foliares de S e de Ca, na época da colheita dos frutos, não foram observados sintomas visuais de deficiência nos frutos. Os teores foliares no início dos sintomas de deficiência e na época da colheita dos frutos foram, respectivamente: N = 8,7 e 6,8; P = 0,70 e 0,32; K = 11,6 e 3,2; Mg = 0,73 e 0,54 g kg-1, e B = 5,8 e 5,5 mg kg-1.The objective of this study was to characterize the visual symptoms of macronutrients and boron deficiencies in the pineapple 'Imperial', and involve them in the foliar diagnosis. The full treatment, - N, - P - K, - Ca, - Mg, - and S - B was applied as nutrient solutions in plastic pots with 14 kg of purified sand beach and a seedling of pineapple, as an experimental unit . The design was a randomized complete block with six replicates. The

  16. Model of pre-harvest quality of pineapple guava fruits (Acca sellowiana (O. berg burret as a function of weather conditions of the crops

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    Alfonso Parra-Coronado

    Full Text Available ABSTRACT Weather conditions influence the quality parameters of pineapple guava fruit during growth and development. The aim of this study was to propose a model of pre-harvest fruit quality as a function of weather conditions in the cultivation area. Twenty trees were flagged per farm in 2 localities of the Department of Cundinamarca, Colombia: Tenjo (2,580 m.a.s.l.; 12.5 °C; relative humidity between 74 and 86%; mean annual precipitation 765 mm and San Francisco de Sales (1,800 m.a.s.l.; 20.6 °C; relative humidity between 63 and 97%; mean annual precipitation 1,493 mm. Measurements were performed every 7 days during 2 harvest periods starting on days 96 (Tenjo and 99 (San Francisco de Sales after anthesis and until harvest. The models were obtained using Excel® Solver, and a set of data was obtained for the 2 different cultivar periods and each study site. The results showed that altitude, growing degree days, and accumulated precipitation are the weather variables with the highest influence on the physicochemical characteristics of the fruit during growth. The models of fresh weight, total titratable acidity, and skin firmness better predict the development of fruit quality during growth and development. Equations were obtained for increases of length and diameter as a function of fruit weight and for days from anthesis as a function of growing degree days and altitude. The regression analysis parameters showed that the models adequately predicted the fruit characteristics during growth for both localities, and a cross-validation analysis showed a good statistical fit between the estimated and observed values.

  17. Empirical model based on Weibull distribution describing the destruction kinetics of natural microbiota in pineapple (Ananas comosus L.) puree during high-pressure processing.

    Science.gov (United States)

    Chakraborty, Snehasis; Rao, Pavuluri Srinivasa; Mishra, Hari Niwas

    2015-10-15

    High pressure inactivation of natural microbiota viz. aerobic mesophiles (AM), psychrotrophs (PC), yeasts and molds (YM), total coliforms (TC) and lactic acid bacteria (LAB) in pineapple puree was studied within the experimental domain of 0.1-600 MPa and 30-50 °C with a treatment time up to 20 min. A complete destruction of yeasts and molds was obtained at 500 MPa/50 °C/15 min; whereas no counts were detected for TC and LAB at 300 MPa/30 °C/15 min. A maximum of two log cycle reductions was obtained for YM during pulse pressurization at the severe process intensity of 600 MPa/50 °C/20 min. The Weibull model clearly described the non-linearity of the survival curves during the isobaric period. The tailing effect, as confirmed by the shape parameter (β) of the survival curve, was obtained in case of YM (β1) was observed for the other microbial groups. Analogous to thermal death kinetics, the activation energy (Ea, kJ·mol(-1)) and the activation volume (Va, mL·mol(-1)) values were computed further to describe the temperature and pressure dependencies of the scale parameter (δ, min), respectively. A higher δ value was obtained for each microbe at a lower temperature and it decreased with an increase in pressure. A secondary kinetic model was developed describing the inactivation rate (k, min(-1)) as a function of pressure (P, MPa) and temperature (T, K) including the dependencies of Ea and Va on P and T, respectively. Copyright © 2015 Elsevier B.V. All rights reserved.

  18. Fruit size and quality of pineapples cv. Vitória in response to micronutrient doses and way of application and to soil covers

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    Aiala Vieira Amorim

    2011-10-01

    Full Text Available The objective of this study was to evaluate the effects of foundation and leaf fertilization with micronutrients on fruit size and quality of pineapple cv. Vitória under the environmental conditions of the Baixo Acaraú irrigated perimeter in Northern Ceará State, Brazil, under two covers (bagana and black plastic of the sandy soil of low fertility. The experimental design was a randomized split blocks one with four levels of soil dressing and four levels of foliar fertilization, with five replications. Micronutrient soil dressing was studied as FTE-12 at doses of 0, 60, 120 and 180 kg ha-1. The four levels of foliar fertilization were: LF0 (without fertilizer, LF 1 (15 leaf fertilization, using the amount of 1158.75 g Fe ha-1, 844.65 g Mn ha-1, 391.5 g ha-1 Zn, 322.65 g ha-1 Cu and 216 g ha-1 B, LF2 (15 leaf fertilization, using twice the quantities of level LF1 and LF3 (15 leaf fertilization, using three times the amount of level LF1. At 13 months after planting the micropropagated plantlets was carried out the floral induction treatment and five months later the fruit harvest determining the following variables: fruit weight and median diameter, soluble solids content (SS and titratable acidity (TA. Both fruit weight and diameter increased with increasing doses of micronutrients applied to the soil and to the leaves, of plants grown both on bagana soil cover and plastic mulch. On the other hand fruit pulp quality was little affected by the treatments studied. There were a small increase of SS contents for plants grown on bagana soil cover and a small decrease of titratable acidity for those grown on plastic mulch, in both cases just in response to micronutrient foliar application.

  19. Effects of ‘flowering chemicals’ application on the development of inflorescence and fruit of pineapple. [Ananas comosus (L. Merr. c.v. Phuket

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    Butrat, P.

    2005-05-01

    Full Text Available This comparative study of the effects of three different flowering chemicals, CaC2, ethephon, and NAA, on the development of inflorescence and fruit of pineapples c.v. Phuket was conducted at Amphoe Talang of Phuket Province, between December 2000 and September 2001. An experiment was arranged as a factorial design in RCB with 2 factors in 3 replications. In the first factor, there were CaC2 1 g/plant, ethephon 100 mg/l combenation with urea 1.5% 60 ml/plant, NAA 0.5 mg (1 ANAA tablet/plant, and the second factor was application period (one time and two times into the shoot tips of nine-month-old ground suckers. The results showed that the application of ethephon one time or two times led to faster floral development than in plants that received NAA or CaC2. In fact, the floral development with ethephon application was 5-7 days faster than that in NAA application, and 8-10 days faster than that in CaC2 application. Apical meristem differentiation took place 3 days after the application of ethephon, followed by complete development of the first floret and stamen within 42-43 days, anthesis within 45-46 days, and pistil within 48-49 days. Maturation of the egg was 5.7-6.3 days later than that of the pollen grains and 2.3-2.6 days after anthesis. In the treatment of NAA application once or twice, the development of the egg cell took a longer period.

  20. Authentication of pineapple (Ananas comosus [L.] Merr.) fruit maturity stages by quantitative analysis of γ- and δ-lactones using headspace solid-phase microextraction and chirospecific gas chromatography-selected ion monitoring mass spectrometry (HS-SPME-GC-SIM-MS).

    Science.gov (United States)

    Steingass, Christof B; Langen, Johannes; Carle, Reinhold; Schmarr, Hans-Georg

    2015-02-01

    Headspace solid phase microextraction and chirospecific gas chromatography-mass spectrometry in selected ion monitoring mode (HS-SPME-GC-SIM-MS) allowed quantitative determination of δ-lactones (δ-C8, δ-C10) and γ-lactones (γ-C6, γ-C8, γ-C10). A stable isotope dilution assay (SIDA) with d7-γ-decalactone as internal standard was used for quantitative analysis of pineapple lactones that was performed at three progressing post-harvest stages of fully ripe air-freighted and green-ripe sea-freighted fruits, covering the relevant shelf-life of the fruits. Fresh pineapples harvested at full maturity were characterised by γ-C6 of high enantiomeric purity remaining stable during the whole post-harvest period. In contrast, the enantiomeric purity of γ-C6 significantly decreased during post-harvest storage of sea-freighted pineapples. The biogenetical background and the potential of chirospecific analysis of lactones for authentication and quality evaluation of fresh pineapple fruits are discussed. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. The effects of concentrate added to pineapple (Ananas comosus Linn. Mer.) waste silage in differing ratios to form complete diets, on digestion, excretion of urinary purine derivatives and blood metabolites in growing, male, Thai swamp buffaloes.

    Science.gov (United States)

    Jetana, T; Suthikrai, W; Usawang, S; Vongpipatana, C; Sophon, S; Liang, J B

    2009-04-01

    Four, male, growing Thai swamp buffaloes (197 +/- 5.3 kg and all 1 year old) were used to evaluate the effects of concentrate added to pineapple waste silage in differing ratios, to form a complete diet, studying in vivo digestion, the rate of passage, microbial protein synthesis and blood metabolites. Animals were fed ad libitum with 4 diets, using four combinations of pineapple waste silage (P) and concentrate (C), in the proportions (on a dry matter basis) of 0.8:0.2 (P80:C20), 0.6:0.4 (P60:C40), 0.4:0.6 (P40:C60) and 0.2:0.8 (P20:C80). The results showed that the intakes of dry matter (DM), organic matter (OM), nitrogen (N), the N-balance, urinary purine derivatives (PD) excretion, the ratios of allantoin to creatinine (CR), PD to CR, the plasma urea-N (PUN) and insulin increased in the animals, but the intake of neutral detergent fiber (NDF), the coefficient of whole tract, apparent digestibility of NDF, the transit time (TT) and the mean retention time (TMRT) decreased, when the proportion of concentrate in the diet increased. This study indicated that the proportion of P40:C60 in the diet produced the best efficiency of urinary PD excretion (mmol) per digestible OM intake (kg DOMI).

  2. Valor nutritivo do resíduo da indústria processadora de abacaxi (Ananas comosus L. em dietas para ovinos. 1. Consumo, digestibilidade aparente e balanços energético e nitrogenado Nutritive value of pineapple by-product (Ananas comosus L. in diets for sheep. 1. Intake, apparent digestibility, energetic and nitrogenous balance

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    M.C.P. Rogério

    2007-06-01

    Full Text Available O presente estudo foi conduzido para avaliar a influência da inclusão do resíduo do processamento de abacaxi (Ananas comosus L. nas dietas sobre os consumos (por unidade de tamanho metabólico - UTM e coeficientes de digestibilidade da matéria seca (MS, matéria orgânica (MO, proteína bruta (PB, extrato etéreo (EE, fibra detergente neutro (FDN, fibra detergente ácido (FDA, hemiceluloses (HCEL, celulose (CEL e energia de dietas experimentais isofibrosas e isoprotéicas. Avaliaram-se também os balanços nitrogenados e de energia dos animais. Vinte ovinos machos, inteiros receberam rações com quatro níveis de inclusão do resíduo (0; 11%; 16%; 27% em base de matéria seca, em delineamento inteiramente ao acaso, com cinco repetições por tratamento. Maiores consumos de matéria seca foram evidenciados quando as rações continham 11% do resíduo. Maiores consumos de proteína bruta, fibra detergente neutro, fibra detergente ácido e celulose (g/UTM e energia metabolizável (kcal/UTM foram apresentados por animais alimentados com dietas contendo 11% de resíduo em relação ao das dietas com 27% de inclusão (PThe influence of increasing levels of pineapple by-product (Ananas comosus L. on intake (unit metabolic size - UMS and apparent digestibility (% of dry matter (DM, organic matter (OM, crude protein (CP, ether extract (EE, neutral detergent fiber (NDF, acid detergent fiber (ADF, hemicelluloses (HCEL and cellulose (CEL and energy of isofibrous and isoproteic experimental diets of sheep were evaluated. Energetic and nitrogenous balance were also measured. Twenty rams were randomly allotted to four treatments in a completely randomized design with five replications. The treatments were formulated to contain zero; 11, 16 and 27% of pineapple by-product on DM basis. Higher dry matter intake was observed on 11% pineapple by-product diet. CP, NDF, ADF, CEL intake (g/UMS and metabolizable energy intake (kcal/UMS of 11% pineapple by

  3. Histologi Tubulus Seminiferus dan Kadar Testosteron Tikus yang Diberi Pakan Imbuhan Tepung Daun Kaliandra dan Kulit Nanas (HISTOLOGY OF SEMINIFEROUS TUBULES AND TESTOSTERONE LEVEL OF RAT GIVEN CALLIANDRA LEAF MEAL AND PINEAPPLE PEELS IN THE DIETS

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    Iriani Setyawati

    2017-09-01

    Full Text Available Calliandra calothyrsus leaf contains 17-28% protein which is hihly potential for use as source of protein supplement for animal feed. However, the plants also contain high level (>10% of condensed tannins as antinutritional effects which may reduce consumtion efficiency of diet. The addition of protease (bromelain into feed containing tannin is expected to decrease the negative effects of tannins. Bromelain can be obtained from the pineapple plant (Ananas comosus including on the peels. This study was conducted to determine the effect of calliandra tannin combined with bromelain protease of pineapple peels in the feed on testicular histology and testosterone level of rats feeded during the growth period. The feeding experiment on post-weaning male rats was conducted using a completely randomized factorial design (4 main factors x 4 subfactors. The main factors were calliandra leaf meal substitution of 0; 10; 17.5 and 25% in the diets and subfactors were addition of pineapple peels, 0; 4.35; 8.70 and 13.05 g/rat/day. Rats were divided into 16 groups and were feeded the diets for two months (during the growth period. The results showed the susbtituion of feed with calliandra leaf had no sigbnificant effecton the thickness of the seminiferous tubules, but it appeared to have significant effect on the histology of seminiferous tubules. Increased pineapple peels level in the diets containing calliandra decreased wall thickness of the seminiferous tubules of the rat testes, indicating that bromalein fastened the maturation of spermatozoa. The addition of pineapple peels into the diet containing calliandra had a significant interaction effect on testoteron levels of male rats, but the testoteron levels among all treated rats were still within the normal range. ABSTRAK Daun kaliandra (Calliandra calothyrsus mengandung protein 17-28% sehingga potensial sebagai sumber protein pakan ternak, namun tanaman ini mengandung condensed tannin cukup tinggi (>10

  4. Aplicação do método QFD na avaliação do perfil do consumidor de abacaxi 'Pérola' Consumer profile evaluation by quality function development for a pineapple

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    Ana Carolina Almeida Miguel

    2007-04-01

    Full Text Available A busca pela longevidade e a procura por alimentos mais saudáveis fizeram com que os consumidores se tornassem cada vez mais exigentes. Diante disso, procurou-se estabelecer o comportamento de compra dos consumidores de abacaxi no Mercado Municipal de Piracicaba (SP através do método de Desdobramento da Função Qualidade (QFD e, com base nas respostas obtidas nos questionários aplicados, traçou-se o perfil dos consumidores, destacando-se suas preferências, costumes, reclamações e exigências. Verificou-se que é a mulher que realiza as compras do abacaxi, com preferência pelo consumo na forma in natura. Encontrou-se insatisfação de 80,1% dos entrevistados quanto à qualidade do abacaxi, sendo a elevada acidez, a principal causa de descontentamento. Logo, a qualidade do abacaxi não correspondia àquela indicada pelos consumidores (casca sem defeitos, coloração amarelada ou mista, tamanho grande, boa aparência interna demonstrando que está sadio, textura firme, aroma característico, baixa acidez, sabor doce, maduro do ponto de vista fisiológico e suculento. Diante da insatisfação dos consumidores e considerando-se que o abacaxi é consumido preferencialmente in natura, deve-se atentar para a preservação da sua aparência e qualidade sensorial. A opinião dos consumidores deve ser considerada na tentativa de identificar os pontos que devem ser melhorados dentro da cadeia de comercialização, a fim de minimizar as perdas e promover a melhoria e a manutenção da qualidade do produto final.The search for longevity and the demand for healthy food have made consumers more conscious. A study was carried out to evaluate the buying habits of pineapple consumers in the Municipal Market of Piracicaba (São Paulo State, Brazil by the Quality Function Development (QFD Method. With the answers from the questionnaire handed to the consumers, it was possible to determine the preferences, habits, complaints and demands. It was verified

  5. Technical classification and evaluation of possible methods for disposal of the wax for coating that leftover during preparation of the pineapple for export

    International Nuclear Information System (INIS)

    Carvajal Torres, Carlos

    2013-01-01

    The search and evaluation of possible methods of disposal are made for the waste of wax, generated during the coating of pineapple. Most suitable processes are characterized by means of tests in the laboratory, with the purpose of to assess the quality of treatment. The information of the possible methods of disposal to be employed has been collected through a literature review. The most suitable method was selected through of an evaluation, by application of a matrix of decision, which, a set of professionals related to this thematic were consulted. The coagulation-flocculation method is chosen according to the criteria considered and a series of evaluations was applied in the laboratory, to define the actual results that were obtained with the application of this treatment. The coagulant concentration and initial pH of the wax were determined statistically, and have had a significant effect on the volume of floc obtained. Also, within of the operating conditions established have been obtained that the optimum dose of flocculant has been 350 mg/L for Iron II sulphate and 400 mg/L for aluminum sulphate. A removal of the 88,7% is obtained for the chemical oxygen demand, 85,3% for the biochemical oxygen demand and 81,1% for fats and oils by employing aluminum sulphate. The yields for the removal of chemical oxygen demand has been of 80,3%, when is used the iron II sulphate, and for biochemical oxygen demand has been of the 80.5%, while the removal of fats and oils has been of 84,9%. A physicochemical process is proposed as a treatment system, and it consists of a roughing channel, a fast mixing zone (coagulation), a mixing zone slowly (flocculation) and a sedimentation. A sand filter of upward flow has complemented the system, followed by an activated carbon bed and horizontal wetland of subsurface flow. Besides, a sludge drying bed has disposed. The cost for construction of the treatment system has estimated of ₡12.700000. A physicochemical characterization of the

  6. Composição mineral da folha em abacaxizeiro: efeito da parte da folha analisada Mineral composition of the leaf in pineapple: effect of the part of the analysed leaf

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    SUSANA CRISTINE SIEBENEICHLER

    2002-04-01

    Full Text Available A padronização da amostragem para a análise foliar em abacaxizeiros é importante para a obtenção de resultados confiáveis. Neste aspecto, este trabalho visa a esclarecer a variação da composição mineral da folha de abacaxi, em plantas desenvolvidas em solo arenoso, sem irrigação. As folhas foram coletadas, limpas e divididas em 4 porções: aclorofilada, basal, mediana e apical. Após a secagem, as amostras foram pesadas, submetidas à digestão e analisadas pela metodologia adequada a cada nutriente. Uma grande variação no teor dos nutrientes foi encontrada ao longo da folha de abacaxi. Uma comparação confiável pode ser estabelecida entre as análises feitas com a folha inteira e a porção clorofilada da folha; o mesmo já não pode ser dito para a porção aclorofilada. Assim, este trabalho vem confirmar a necessidade da padronização da amostragem.The standardization of the sampling for foliar analysis in pineapple is important to obtain reliable results. In this aspect this work seeks to identify the variation of the mineral composition of pineapple leaf, in plants developed in sandy soil, without irrigation. The leaves were collected, cleaned and divided in 4 parts: non-chlorophyllated, basal, medium and apical. After the drying, the samples were weighted, digested, and analyzed by the appropriate methodology for each nutrient. A great variation in the concentration of the nutrients was found along the pineapple leaf. A reliable comparison of the analysis can be established between the whole leaf and the chlorophyllated portion of the leaf; the same does not apply to the non-chlorophyllated portion of the same leaf. This paper confirms the need of the standardization of leaf sampling for mineral composition evaluation.

  7. Suscetibilidade a dano pelo frio em abacaxi 'pérola' tratado com 1-metilciclopropeno Susceptibility to chilling injury for 'pérola' pineapple treated with 1-methylcyclopropene

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    Ovídio Ricardo Dantas Júnior

    2009-03-01

    Full Text Available Este trabalho avaliou o efeito de 1-metilciclopropeno (1-MCP na suscetibilidade ao dano pelo frio em abacaxi 'Pérola', colhido em Santa Rita-PB, na maturidade comercial. Os frutos foram tratados com 1-MCP (0; 300; 600 e 1.200 ppb, por 12 horas, sob condição ambiente e armazenados: a durante 42 dias a 10ºC, avaliados a cada 7 dias, e quando transferidos para o ambiente (25ºC e 65±5% U.R., após 21; 28; 35 e 42 dias, e também foram avaliados após sete dias; b durante 32 dias, a 7ºC, avaliados a cada 8 dias, e transferidos para o ambiente após 8; 16; 24 e 32 dias, sendo avaliados após sete dias. Em frutos mantidos a 10ºC, não se observou efeito do 1-MCP em retardar a perda de qualidade. Para frutos a 7ºC, o 1-MCP minimizou a incidência de dano pelo frio quando transferidos para a condição ambiente.This work evaluated the effect of 1-methylcyclopropene (1-MCP on susceptibility to chilling injury (CI in 'Perola' pineapple, harvested at the commercial maturity from Santa Rita municipality, Paraíba State, Northeast Brazil. Fruits were treated at room temperature with 1-MCP (0, 300, 600, and 1200 ppb for a 12-hour period and stored: a during 42 days at 10ºC, evaluated each 7 days and, when transferred to room conditions (25ºC and 65±5% R.H., followed 21, 28, 35, and 42 days, and were also evaluated after 7 days; b during 32 days at 7ºC, evaluated at 8-day intervals, transferred to room conditions followed 8, 16, 24, and 32 days, and evaluated after 7 days. For pineapple kept at 10ºC it was not observed 1-MCP effect of retarding the loss of fruit quality. For fruits stored at 7ºC, 1-MCP minimized the incidence of CI when pineapples were transferred to room conditions.

  8. Avaliação da qualidade do abacaxi "Pérola" minimamente processado armazenado sob atmosfera modificada Evaluation of quality in fresh-cut 'Pérola' pineapple stored under modified atmosphere

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    Juliana Costa Bueno Santos

    2005-04-01

    Full Text Available Objetivou-se com este trabalho verificar o efeito da atmosfera modificada ativa sobre a qualidade do abacaxi minimamente processado. Assim, foram avaliadas as modificações físico-químicas, sensorial e microbiológica do abacaxi 'Pérola' minimamente processado submetido a diferentes atmosferas modificadas passiva e ativamente (controle - atmosfera modificada passiva; 5%O2 + 5%CO2 , 2%O2 + 10%CO2. A atmosfera com 2% O2 + 10% CO2 determinou menor perda de líquido drenado, embora tal perda tenha sido desprezível. A atmosfera modificada ativa preveniu o amaciamento do abacaxi minimamente processado, reduzindo a atividade da poligalacturonase e solubilização péctica. As variáveis pH e vitamina C não foram significativamente afetadas pela atmosfera modificada ativa. Maiores valores de sólidos solúveis totais (SST foram encontrados nos frutos sob atmosfera 5% O2 + 5% CO2 e no caso dos açúcares solúveis o tratamento com 2% O2 + 10% CO2 resultou em maiores teores. De acordo com análise sensorial, os frutos sob atmosfera modificada passiva se sobressaíram com relação ao sabor, aparência e cor. A análise microbiológica detectou valores insignificantes para fungos filamentosos, leveduras e coliformes a 35° e 45°C em todos os tratamentos. Conclui-se que o abacaxi 'Pérola' minimamente processado pode ser armazenado por 8 dias, a 5°C, sob atmosfera modificada passiva.The goal of this work was to verify the effect of active modified atmosphere on the quality of fresh-cut pineapple. Physical-chemical, sensory and microbiological changes of fresh-cut 'Pérola' pineapple treated with passive (control and active (5%O2 + 5%CO2; 2%O2 + 10%CO2 modified atmosphere were studied. The 2%O2 + 10%CO2 combination promoted the lowest loss of drained liquid, although they were negligible. Active modified atmosphere prevented the softening of fresh cut pineapples, reducing the poligalacturonase activity and pectin solubilization. The pH and ascorbic

  9. Study of modification of fibers from pineapple crown for the formation of polymeric composites;Estudo da modificacao da fibra proveniente da coroa de abacaxi para a formacao de compositos polimericos

    Energy Technology Data Exchange (ETDEWEB)

    Marcon, Juliana S.; Mulinari, Daniella R.; Cioffi, Maria Odila H.; Voorwald, Herman J.C., E-mail: mat07103@feg.unesp.b [Universidade Estadual Paulista (UNESP), Guaratingueta, SP (Brazil)

    2009-07-01

    Study of modification of fibers from pineapple crown for the formation of polymeric composites An important aspect to make fiber and matrix work together in a given application is the interface between them. For an efficient adherence fiber/matrix an appropriate interfacial contact is required. For this purpose, it was made a modification in the fiber surface using sodium hydroxide solution. And the effect of fibers modification was analyzed by X-Ray diffractometry (XRD) and scanning electron microscopy (SEM). The results indicated that occurred an effective increase in the crystallinity of modified fibers compared to natural fibers and that was occurred the formation of pores or holes across the rough surface of the fiber showing that will can occur an increase in effective superficial area for contact with polymeric matrix. (author)

  10. Indicadores de estresse salino em abacaxizeiro cultivado na ausência e presença de fitorreguladores Indicators of salinity in pineapple grown in the absence and presence of growth regulators

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    Yuri Lima Melo

    2011-09-01

    Full Text Available O objetivo do presente estudo foi avaliar o efeito de diferentes concentrações de NaCl nas fases de multiplicação e enraizamento in vitro do abacaxizeiro cultivar MD Gold. Brotos de abacaxizeiro foram inoculados em meio MS, na ausência e presença dos fitorreguladores ácido naftalenoacético (ANA e 6-benzilaminopurina (BAP e também de diferentes concentrações de NaCl (Controle - 0; 50; 100 e 150 mM. Mensalmente, os brotos foram subcultivados e foram analisados altura média, número de folhas vivas e mortas, taxas de brotação e enraizamento. Durante a multiplicação e na ausência de ANA e BAP, o NaCl provocou significativa redução no crescimento e desenvolvimento de brotos de abacaxizeiro, expresso pela altura e pelo número de folhas, nas doses mais elevadas, contrariamente, na presença dos fitorreguladores. Durante os 60 dias iniciais, ocorreu aumento na produção de folhas. Contudo, aos 90 dias, observou-se decréscimo na média de folhas vivas nos brotos tratados com sal. O cultivo in vitro de abacaxizeiro em presença de sal é mais eficiente na presença de ANA e BAP, garantindo a manutenção do crescimento, aumentando o número de folhas, produzindo novas gemas e acelerando o processo de enraizamento.To assess the effects of salt on the pineapple MD Gold during the multiplication and rooting phases in vitro, this study evaluated its performance in different concentrations of NaCl in the absence or presence of growth regulators. Pineapple shoots were inoculated on MS solution in the absence and presence of the growth regulators naphtaleneacetic acid (NAA and 6-benzlaminopurine (BAP and different concentrations of NaCl (Control - 0, 50, 100 and 150 mM. Monthly, shoots were subcultured and it was analyzed height, number of alive and dead leaves, rates of sprouting and rooting. During the multiplication in the absence of NAA and BAP, the NaCl treatments caused significant reduction in growth and development of pineapple

  11. Qualidade sensorial dos frutos do abacaxizeiro 'imperial' cultivado em deficiência de macronutrientes e de boro Sensorial quality of the fruits of 'imperial' pineapple cultivated in macronutrient and boron deficiencies

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    Maria José Mota Ramos

    2010-09-01

    Full Text Available A comercialização do abacaxi vem expandindo-se no mercado mundial principalmente por suas apreciáveis características de sabor, aroma e cor. A nutrição mineral da planta exerce uma influência acentuada na composição química do abacaxi. A inexistência de informações sobre a influência da deficiência nutricional na qualidade dos frutos do abacaxizeiro 'Imperial' motivou a realização de um experimento em casa de vegetação da Universidade Estadual do Norte Fluminense, em Campos dos Goytacazes-RJ, no período de 2003 a 2005, para avaliar os efeitos das deficiências de macronutrientes e de boro na composição fisico-química e na qualidade sensorial dos frutos dessa cultivar. A unidade experimental constou de um vaso de plástico com 14 L de areia purificada e uma muda de abacaxizeiro. Foram utilizados os seguintes tratamentos: completo, - N, - P, - K, - Ca, - Mg, - S e - B, aplicados sob a forma de soluções nutritivas, em blocos casualizados completos, com seis repetições. As seguintes características foram obtidas nos frutos: firmeza, teor de suco, AT, SST, SST/AT, vitamina C, pH, coloração e a análise sensorial da polpa. As deficiências de N e de K aumentaram a firmeza da polpa, mas a de S a diminuiu. As deficiências de N e S aumentaram a AT e o teor de Vitamina C, mas diminuíram SST/AT, a coloração da polpa e o pH. A deficiência de K reduziu os SSTs e a de Ca e de S os aumentou. As deficiências de N e K reduziram a aceitação sensorial dos frutos. As deficiências de P, Mg e B não alteraram as propriedades sensoriais do abacaxi.The marketing of pineapple has been expanding in the world mainly by its appreciable characteristics of flavor, aroma and color. The mineral nutrition of the plant exert an accentuated influence on the chemical composition of pineapple. The inexistence of information on the influence of the mineral deficiency on the quality of the 'Imperial' pineapple fruits motivated the accomplishment

  12. Influence of irradiation on the degradation speed of pre servants of sliced pineapple preserved by a combined method; Influencia de la irradiacion sobre la velocidad de degradacion de preservantes en rodajas de pina conservadas por metodos combinados

    Energy Technology Data Exchange (ETDEWEB)

    Garcia A, A; Castro, D; Espinosa, R; Gonzalez, E; Fernandez, L; Garcia R, M O

    1996-12-31

    In the fulfillment of this study, pineapple of the spanish red variety, cut in 1.0 - 1.5 cm slices, were used. The product was sunken in a saccharose syrup with the addition of sodium methabisulphite and potassium sorbate and packed in polyethylene bags and latter irradiated with 0.2 and KGy doses. The behavior of potassium sorbate with two addition levels is similar during storage at room temperature for the studied irradiation doses. The degradation process of sodium methabisulphite takes place almost totally at one month storage. Unlike of potassium sorbate there exist no meaning differences between sulfur dioxide in irradiated and nonirradiated samples.It is concluded that the irradiation process cause an increasing in the degradation of potassium sorbate basically at the beginning of the storage, while seems no exert some effect on the sodium methabisulphite degradation. (Author).

  13. Estiolamento na micropropagação do Abacaxizeiro cv. Pérola Etiolated in micropropagation of cv. Pérola Pineapple plant

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    Maria Aparecida Moreira

    2003-10-01

    Full Text Available Objetivou-se estudar o efeito do estiolamento na micropropagação de abacaxizeiro cv. Pérola e posterior recuperação dos brotos estiolados, realizando-se dois experimentos. No primeiro, os caules utilizados como explantes foram obtidos de brotos pré-estabelecidos in vitro, dos quais foram retiradas as folhas. O estiolamento foi induzido colocando-se os explantes em tubos de ensaio no escuro por 20, 40 e 80 dias, contendo os seguintes meios de cultura: 1 MS suplementado com 0,1 mg.L-1 de ANA e 0,5 mg.L-1 de BAP, 2 MS suplementado com 1,8 mg.L-1 de ANA e 2 mg.L-1 de BAP e 3 MS sem reguladores de crescimento. Para número de brotos, o melhor meio foi o MS + 1,8 mg.L-1 de ANA e 2 mg.L-1 de BAP, obtendo-se média de 10,26 brotações aos 40 dias no escuro. Para comprimento de brotos estiolados, o meio MS sem reguladores de crescimento foi significativamente melhor quando os explantes foram mantidos por 80 dias no escuro, apresentando comprimento médio de 10,86 cm. No segundo experimento, os brotos estiolados, com e sem o ápice, foram colocados horizontalmente em placas contendo meios de cultura com idêntica formulação aos descritos anteriormente. Para número total de brotos, brotações estioladas sem ápice em meio MS suplementado com 1,8mg.L-1 de ANA e 2 mg.L-1 de BAP promoveram o melhor resultado, correspondendo a 10,61 brotações por explante.It was aimed to produce micropropagated plantlets of pineapple cv. Pérola by using the etiolated technique and subsequent recovery of etiolated shoots. Two experiments were carried out. In the first, the stalks used as explant were obtained from in vitro shoots established without leaves. The etiolation was induced by putting the explants in test tubes in the darkness for 20, 40 and 80 days with the media: 1 MS supplemented with ANA 0.1mg.L-1 and BAP 0.5mg.L-1, 2 MS supplemented with ANA 1.8mg.L-1 and BAP 2mg.L-1 and 3 MS without growth regulators. The best results for shoot number were obtained

  14. Brassinosteróide e substratos na aclimatização do abacaxizeiro ‘Imperial’ = Brassinosteroid and substrates in acclimatization of ‘Imperial’ pineapple

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    Paulo Henrique Aragão Catunda

    2008-07-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito da aplicação de diferentes concentrações de um análogo de brassinosteróide (BIOBRAS – 16 e de dois substratos sobre o crescimento de mudas micropropagadas do abacaxizeiro ‘Imperial’. O experimento foi conduzido em DBC, em esquema fatorial 2 x 5 x 4, no qual foram avaliadas cincoconcentrações do BIOBRAS - 16 (0; 0,1; 0,3; 0,5 e 1 mg L-1, dois tipos de substratos e quatro épocas de amostragem (60, 90, 120 e 150 dias após transplantio. Os substratos utilizados foram o Plantmax® e outro substrato obtido pela compostagem de uma mistura entre o bagaço-de-cana e torta de filtro (BT. As plântulas foram transplantadas para tubetes cônicos em casa-de-vegetação equipada com nebulizadores intermitentes. As plantas cultivadas no substrato BT e pulverizadas com 0,1 mg L-1 do BIOBRAS - 16 apresentaram maior crescimento da parte aérea, com maior número de folhas, diâmetro de roseta, largurade folha, massa fresca e massa seca, aos 150 dias após transplantio. Nas plantas cultivadas no substrato BT as pulverizações com o BIOBRAS-16 a 0,1 mg L-1 proporcionaram acúmulo de matéria seca 2,8 vezes superior ao valor da testemunha cultivada no substrato Plantmax®. A massa fresca e seca de raízes foram superiores no substrato Plantmax® em relação ao BT, nas últimas épocas de amostragem.This work aimed to evaluate the effects of different concentrations of a brassinoesteroid analogue (BIOBRAS-16 and two substrates on the growth of micropropagated seedlings of ‘Imperial’ pineapple. A randomized block design was used in a 2 x 5 x 4 factorial scheme, evaluating five concentrations of BIOBRAS-16 (0; 0.1; 0.3; 0.5and 1 mg L-1, two types of substrates and four sampling periods (60, 90, 120 and 150 day safter planting. The substrates used were Plantmax® and another obtained from a mix of composting sugar-cane bagasse and filter cake (CC. The seedlings were transplanted tosmall conic tubes and

  15. Evaluation of the effects of gamma radiation on the quality of pineapple (Ananas comosus (L.) Meer) cv. Smooth Cayenne minimally processed, storaged on differents temperatures and packages;Avaliacao dos efeitos da radiacao gama na qualidade de abacaxi (Ananas comosus (L.) Meer) cv. Smooth Cayenne minimamente processado, armazenado em diferentes temperaturas e embalagens

    Energy Technology Data Exchange (ETDEWEB)

    Leite, Daniela Terenzi Stuchi

    2006-07-01

    The present work aimed to verify the effects of gamma radiation (doses until 2 kGy), types of packages and temperatures of storage (5, 8 e 12 deg C) on the physicochemical characteristics, on the microbiological contamination and on the sensorial characteristics of pineapple 'Smooth Cayenne' minimally processed. The fruits were selected, washed; peeled and cutted transverse and the slices cutted were cutted on two or for pieces. The pieces were immersed in chlorinated water (100 mg/L) for 3 minutes, flowing and package, irradiated and stored. According with the results obtained in thi present work it was concluded that bigger the temperature of storage more quickly were the browning of the fruits. The loss of fresh weight of pineapple was bigger in the packages of polystyrene comparing with PET package when both are covered with PVC film and smaller in the PET packages covered with the same material. Doses of gamma radiation until 2 kGy did not change the physico-chemical and sensorial characteristics of pineapple 'Smooth Cayenne' minimally processed. The microbiological growth on the pieces of the processed fruit was smaller on the biggest dose. All the samples were is in good conditions for the human consume. (author)

  16. Captura de Rhynchophorus palmarum L. (Coleoptera: curculionidae em armadilhas iscadas com o feromônio de agregação e compostos voláteis de frutos do abacaxi Trap catches of Rhynchophorus palmarum L. (Coleoptera: curculionidae baited with its aggregation pheromone and volatile compounds from pineapple

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    Adriana Guimarães Duarte

    2003-04-01

    Full Text Available O objetivo deste trabalho foi determinar os índices de captura de Rhynchophorus palmarum em armadilhas iscadas com o feromônio de agregação, 6-metil-2(E-hepten-4-ol (rincoforol, associado a toletes de cana-de-açúcar, a pedaços de frutos do abacaxi e a seis compostos voláteis isolados de frutos do abacaxi. Os compostos voláteis do abacaxi são caracterizados por uma mistura de ésteres metílicos e etílicos, sendo o octanoato de metila e o octanoato de etila os mais abundantes. As armadilhas iscadas com o rincoforol associado a toletes de cana-de-açúcar e as iscas com rincoforol associado a pedaços de abacaxi não apresentaram diferenças significativas no número de besouros capturados. No entanto, ambas apresentaram índices de captura superiores àquelas em que o rincoforol foi utilizado em associação com voláteis do abacaxi. Não se observaram efeitos significativos do local e época de captura, nem no número de machos e de fêmeas capturados.The aim of this work was to investigate the capture of Rhynchophorus palmarum in traps baited with its aggregation pheromone, 6-methyl-2(E-hepten-4-ol (rhynchophorol, in association with sugar cane, pieces of pineapple fruit, and six volatile compounds from pineapple. A mixture of methyl and ethyl esters, being methyl octanoate and ethyl octanoate the most abundant, characterizes the volatile compounds from pineapple fruits. Traps baited with rhynchophorol in association with sugar cane and those baited with rhynchophorol in association with pieces of pineapple, showed no significant differences in the number of trapped weevils. However, both traps caught significantly more weevils, than those baited with rhynchophorol in association with pineapple volatiles. There were no significant effects from place and time or in the number of male and female weevils trapped.

  17. In vitro cultivation of pineapple seedlings using filters, artificial ventilation and sucrose = Cultivo in vitro de plântulas de abacaxizeiro com uso de filtros, ventilação artificial e sacarose

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    Patrícia dos Santos Mendes

    2015-06-01

    Full Text Available An alternative to the production of quality seedlings is the use of in-vitro systems of propagation. However, the conventional in-vitro environment is different from the external environment, and is often the reason for physiological and morphological disorders in the plant. To this effect, the aim of this study was to evaluate the use of filters and artificial ventilation to increase air exchange between the flask and environment, and adjust the concentration of sucrose in the culture medium for a better development of the photosynthetic apparatus, and stimulate the growth of pineapple plants in vitro. The experimental design was completely randomised in a 3 x 3 factorial scheme with nine replications. The factors under study consisted of three concentrations of sucrose (0, 15, and 30 g L-1 and three methods for ventilating the flask (unfiltered flask with no ventilation, filtered flask with no ventilation, and filtered flask with ventilation. The use of unfiltered flasks with no ventilation and 15 g L-1 of added sucrose, result in greater growth of the pineapple plants in vitro. The use of filtered flasks with ventilation promotes an increase in the levels of chlorophyll-a, chlorophyll-b, and total chlorophyll, as well as improvements in the system of photosynthesis of the pineapple seedlings = Uma alternativa para a produção de mudas de qualidade é a utilização de sistemas de propagação in vitro . Porém, o ambiente de cultivo convencional in vitro é diferente do ambiente externo e comumente há razão de distúrbios fisiológicos e morfológicos na planta. Neste contexto, objetivou-se com o presente trabalho avaliar o uso de filtros e ventilação artificial visando aumentar as trocas de ar do frasco com o ambiente e ajustar a concentração de sacarose no meio de cultura para o melhor desenvolvimento do aparelho fotossintético e estímulo do crescimento das plantas in vitro de abacaxizeiro. O delineamento experimental foi

  18. Frutas estruturadas de umidade intermediária obtidas de polpas concentradas de abacaxi, manga e mamão Restructured fruits of intermediate moisture from concentrated pineapple, mango and papaya pulps

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    Regina Kitagawa Grizotto

    2005-12-01

    Full Text Available O objetivo deste trabalho foi estabelecer procedimento tecnológico para produção de fruta estruturada com elevados teores (450 a 700 g/kg de polpas concentradas de abacaxi (39ºBrix, manga (35ºBrix e mamão (16ºBrix, e quantidade mínima de sacarose, utilizando alginato ou pectina baixamente metoxilada. Glicose ou glicerol foi utilizado como supressor de atividade de água. Valores de firmeza de 240 g para géis de alginato e 1300 g para géis de pectina, determinados em Analisador de Textura TA.XT2, podem ser utilizados como indicadores para a estruturação, baseando-se nas propriedades de corte das frutas estruturadas. O processo de estruturação compreende a mistura sob vácuo (88 kPa da polpa concentrada previamente aquecida a 60ºC e o hidrocolóide disperso em açúcar. Soluções de CaHPO e Glucona-Delta-Lactona foram adicionadas à mistura para favorecer a formação do gel. Os resultados mostraram a importância de elevar o pH de polpas de abacaxi e manga para 4,00 com NaOH. O alginato produziu 4 géis mais firmes e o glicerol foi o supressor de atividade de água mais eficiente. As frutas estruturadas de mamão com alginato apresentaram maior firmeza (acima de 3 kg. Os géis de abacaxi com alginato não puderam ser cortados com a faca, embora os géis de manga puderam ser cortados, eles aderiram à faca.The objective of this research was to establish a technological procedure for the production of restructured fruits with high contents (450 to 700 g/kg of concentrated pineapple (39ºBrix, mango (35ºBrix and papaya (16ºBrix pulps, and minimal amounts of sucrose using alginate or low methoxy pectin. Glycerol or glucose was used to suppress the water activity. Firmness values of 240 g for alginate gels or 1300 g for pectin gels, measured using the TA.XT2 Texture Analyser, can be used as indicators for structuring based on the cutting properties of the structured fruits. The structuring process includes the mixing under vacuum (88

  19. Resposta de plantas micropropagadas de abacaxizeiro à inoculação de bactérias diazotróficas em casa de vegetação Response of pineapple plantlets to inoculation with diazotrophic bacteria in greenhouse

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    Olmar Baller Weber

    2003-12-01

    Full Text Available O objetivo deste trabalho foi selecionar e avaliar bactérias diazotróficas isoladas de abacaxizeiro (Ananas comosus (L. Merril no desenvolvimento de cultivares micropropagadas da mesma espécie em casa de vegetação. Plantas da cultivar Perolera foram submetidas à inoculação com Asaia bogorensis (AB219 e cultivadas em tubetes, durante 145 dias, com as misturas: casca de arroz carbonizada, folha de carnaubeira triturada e vermicomposto; casca de arroz carbonizada, pó da casca do coco maduro e vermicomposto; casca de arroz carbonizada, vermiculita e vermicomposto. Plantas da cultivar Primavera receberam inóculos com o AB219 e bactérias relacionadas a Burkholderia cepacia (AB202 e AB213, enquanto plantas das cultivares Pérola e Smooth Cayenne receberam AB219 e AB213, sendo cultivadas, por 140 dias, em tubetes com a mistura de vermicomposto e vermiculita. A colonização dos abacaxizeiros pelas bactérias diazotróficas foi confirmada. As plantas da cultivar Perolera cresceram melhor em casca de arroz carbonizada, vermiculita e vermicomposto e responderam positivamente ao AB219. Já as plantas da cultivar Primavera não apresentaram resposta significativa à inoculação com AB219, AB202 e AB213. Houve incremento de 23,1% a 38,5% na matéria seca de raízes das plantas da cultivar Pérola na presença de AB213 e AB219, respectivamente. A presença de AB213 incrementou em 15,2% a matéria seca da parte aérea das plantas da cultivar Smooth Cayenne. Os resultados revelam a eficiência de bactérias diazotróficas na promoção do crescimento de abacaxizeiros.The objective of this work was to select and to evaluate isolates of diazotrophic bacteria associated to pineapple (Ananas comosus (L. Merril plants in the development of micropropagated plantlets of cultivars pineapple in greenhouse. Plantlets from the Perolera cultivar have been submitted to inoculation with Asaia bogorensis (AB219 and were cultivated during 145 days on the following

  20. Avaliação do desenvolvimento de diferentes tamanhos de mudas micropropagadas de abacaxizeiro, após aclimatação Evaluation of the development of different sizes of micropropagated seeedlings of pineapple tree, after acclimatized

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    Sávio da Silva Berilli

    2011-03-01

    Full Text Available A micropropagação de plantas é extremamente útil na multiplicação de novas cultivares em larga escala. O abacaxizeiro 'Vitória' é uma cultivar com a importante característica de ser resistente à fusariose. Após cultivo in vitro e aclimatização, é possível produzir mudas de diferentes tamanhos, entretanto um tamanho-padrão tem sido utilizado para comercialização. O uso de mudas de diferentes estádios de crescimento, após a aclimatização, pode interferir na qualidade final das mudas para o plantio no campo. O objetivo deste trabalho foi avaliar o efeito dos diferentes tamanhos de mudas do abacaxizeiro 'Vitória', após aclimatização, na qualidade dessas após o período de aclimatação. Foram selecionadas mudas micropropagadas do abacaxizeiro 'Vitoria', aclimatizadas em casa de vegetação com diferentes tamanhos, e estas foram plantadas em canteiros a céu aberto para aclimatação, por um período de 150 dias. Foi utilizado o delineamento experimental em blocos casualizados, em arranjo fatorial 5x2, correspondentes a cinco períodos de aclimatização das mudas (30; 60; 90; 120 e 150 dias e a duas épocas de avaliação (início e aos 150 dias da aclimatação. Os tratamentos foram avaliados em relação ao número de folhas, área foliar, altura da planta, diâmetro de roseta, comprimento da folha mais desenvolvida, massa seca e massa fresca da parte aérea. Para as condições deste experimento, verificou-se que mudas com 60; 90; 120 e 150 dias após aclimatização apresentam condições para aclimatação.The micropropagation of plants is extremely useful in the multiplication of new cultivars in wide scale. The pineapple tree Vitória is a cultivar with the important characteristic of being resistant to the fusariosis. After cultivation in vitro and acclimatization is possible to produce seedlings of different sizes, meantime a standard size has been used for marketing. The use of seedlings of different stages of

  1. Avaliação da vanilina como agente antimicrobiano em abacaxi 'Pérola' minimamente processado Evaluation of vanillin as an antimicrobial agent on fresh-cut 'Pérola' pineapple

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    Lucimara Rogéria Antoniolli

    2004-09-01

    Full Text Available Procurou-se avaliar os efeitos da vanilina como agente antimicrobiano, bem como o nível de injúria física como fator de contaminação inicial em abacaxi (Ananas comosus (L. Meer cv. Pérola minimamente processado. Fatias e cubos foram obtidos a partir de frutos sanitizados, descascados e fatiados mecanicamente. Os dois tipos de corte foram imersos, separadamente, em água (controle ou soluções de vanilina 3000 ou 5000mg.L-1, durante 30 segundos. Após período de repouso, para drenagem do excesso de líquido, foram acondicionados em embalagens de polietileno tereftalato e mantidos à temperatura de 4 ± 1°C durante 12 dias. As análises microbiológicas, realizadas em intervalos de 3 dias, envolveram a contagem de microrganismos aeróbios mesófilos e de bolores e leveduras e a determinação de coliformes totais e fecais. A utilização de vanilina mostrou-se ineficiente no controle do crescimento da população de microrganismos aeróbios mesófilos e de bolores e leveduras em abacaxi 'Pérola' minimamente processado. O maior nível de injúrias físicas efetuado nos cubos parece favorecer a contaminação inicial do produto.The purpose of this research was to evaluate the effects of vanillin as an antimicrobial agent, as well as the relationship between the injury degree and initial contamination in fresh-cut pineapple (Ananas comosus (L. Meer cv. Pérola. Slices and cubes were obtained from whole fruits that were mechanically peeled and sliced after sanitization. Both kinds of cutting were dipped in pure water (control or vanillin solutions 3000 or 5000mg.L-1, for 30 seconds. After that, the liquid was drained, slices and cubes were placed in polyethylene terephtalate packages and stored at 4 ± 1°C during 12 days. Microbiological analyses were carried out every 3 days and involved mesophile aerobic counts, molds and yeasts and total and fecal coliforms determination. The use of vanillin was inefficient for the control of mesophile

  2. Estudo da estabilidade físico-química de suco de abacaxi 'pérola' Study of the psycho-chemical stability of 'Pérola' pineapple juice

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    Paulo Rogério Siriano Borges

    2011-08-01

    Full Text Available Neste trabalho foram avaliadas as mudanças físico-químicas provocadas pelo tipo de armazenamento do suco de abacaxi 'Pérola', durante de 48 horas após sua elaboração. Foram avaliadas 4 condições de armazenamento, em ambiente refrigerado (4 ±1º C e em temperatura ambiente (22 ±1º C, ambas com e sem luminosidade. O experimento foi conduzido em delineamento inteiramente casualizado, com os tratamentos dispostos em esquema fatorial 2 x 2 x 6, sendo 2 ambientes de luminosidade (presença e ausência, 2 condições de temperatura (refrigeração e ambiente e 6 tempos de amostragem (0, 6, 12, 24, 36 e 48 horas com 3 repetições. Foram analisados, pH, acidez titulável, sólidos solúveis, vitamina C total e cor utilizando-se as variáveis claridade (L*, ângulo de cor (hº e cromaticidade (C*. O tempo de armazenamento apresentou influência negativa na qualidade do suco provocando a diminuição dos teores de ácido ascórbico e ácido cítrico, alterações na cor, principalmente o escurecimento. Entretanto, quando armazenado em ambiente refrigerado e sem luz, o suco conservou suas características iniciais por mais tempo, sendo estas melhor preservadas pelas baixas temperaturas.In this work, psycho-chemical changes caused by the storage of homemade 'Pérola' pineapple juice, over 48 hours after its preparation were evaluated. Four storage conditions were evaluated in a refrigerated environment (4 ±1º C and in room temperature (22 ±1º C both in the presence and abscence of light. The experiment was conducted with a totally random design, with treatments arranged in a factorial 2 x 2 x 6, with 2 light conditions (presence and absence, 2 temperature conditions (cooling and room temperature and 6 sampling times (0, 6, 12, 24, 36 and 48 hours with three replicates. pH, titratable acidity, soluble solids, total vitamin C and color using the variables: luminosity (L*, hue angle (hº and chromaticity (C* were analyzed. The period of storage

  3. Qualidades físicas e químicas do abacaxi comercializado na CEAGESP São Paulo Physico chemical quality of pineapple commercialized in the CEAGESP-São Paulo

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    Fábio José Bengozi

    2007-01-01

    casca, pode representar um avanço significativo na indicação da qualidade dos frutos comercializados, o que permite concluir ser desnecessário o uso de etefon na fase de pré-colheita.The purpose of the present study was to analyze the variations of the physical and chemical characteristics of pineapple fruits commercialized at the "Entreposto Terminal de São Paulo" (ETSP, of the "Companhia de Entrepostos e Armazéns Gerais de São Paulo" (CEAGESP, from September/2005 to March/2006. Evaluations were made at the CEAGESP and in the laboratories of the Agro industry, Foods and Nutrition Department of the "Escola Superior de Agricultura Luiz de Queiroz" - ESALQ/USP. Samples were collected from three different wholesalers at random, fortnight, directly from the items provided for commercialization. Pineapples were standardized as type 10 (boxes with 10 fruits collected from each wholesaler/origin. The following characteristics were evaluated: weight of whole pineapple, weight of crown tuft, skin color, density, pulp color, pH value, contents of soluble solids (SS, titratable acidity (TA and the relation SS/TA. Statistical analysis was made with Program SAS. Results showed that among the regions where pineapple grows, stood out in relation to offer regularity the regions of: Canápolis (cv. 'Smooth Cayenne' Sapé and Miracema do Tocantins (cv. 'Pérola'. The other areas that produce the cultivar 'Pérola' presented great variations in the weight average of the fruit commercialized, indicating technological differences among the production systems (where the fruit showed a considerable increase in weight. Throughout the period of analysis great statistic variation in the contents of soluble solids was noticed in the areas of Canápolis (cv. 'Smooth Cayenne' and Itaberaba, Sapé and Miracema (cv. 'Pérola'. Among the chemical variables analyzed, the use of the manual refractometer for determining the contents of soluble solids per sample, associated with apparent ripening

  4. Utilização de abacaxi (Ananas comosus L. cv. Pérola e Smooth cayenne para a produção de vinhos: estudo da composição química e aceitabilidade Utilization of pineapple (Ananas comosus L. cv. Pérola and Smooth cayenne for wine making: study of chemical composition and acceptance

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    Kátia Gomes Lima Araújo

    2009-03-01

    Full Text Available Foram estudadas as características químicas de vinhos produzidos com as cultivares de abacaxi Pérola e Smooth cayenne, com vistas a predizer a qualidade das bebidas, tomando-se como base a sua composição e aceitabilidade. Os mostos de abacaxi foram analisados quanto ao pH, acidez total, ácidos orgânicos fixos, açúcar total, pectina total, compostos fenólicos, K, Ca, Fe, Cu, N e P, enquanto nos vinhos analisou-se também a acidez volátil, teor alcoólico, alcoóis superiores, acetaldeído, acetato de etila, glicerol, metanol e cor. Os resultados indicaram que existem semelhanças em relação à composição química de mostos e vinhos de abacaxis e uvas. A análise dos compostos voláteis formados durante a fermentação alcoólica indicou que a composição do mosto de abacaxi interferiu nas quantidades de alguns voláteis produzidos pela levedura Saccharomyces cerevisiae. O teste de aceitabilidade com escala hedônica de nove pontos indicou que os vinhos elaborados com as duas cultivares de abacaxi foram igualmente aceitos.The objective of the present work was to study the chemical characteristics of wine made with two different varieties of pineapple: Pérola and Smooth cayenne, in order to predict its quality based on composition and acceptance. The pineapple musts were chemically characterized through the analysis of pH, total acidity, fixed organic acids, total sugar, total pectin, phenolic compounds, and K, Ca, N e P. The wines were characterized by volatile acidity, alcoholic grade, higher alcohols, acetaldehyde, ethyl acetate, glycerol, methanol, and color. The results showed that there are similarities between the pineapple and grape musts and wines. The analysis of volatile compunds performed during the alcoholic fermentation showed that the pineapple must composition interfered with the quantities of volatiles formed by Saccharomyces cerevisiae. The 9-point hedonic scale acceptance indicated that the two varieties of wine

  5. DESBASTE DE MUDAS TIPO FILHOTE DO ABACAXI CV. PÉROLA: 1. PRODUÇÃO E QUALIDADE DO FRUTO SLIPS THINNING FROM THE PINEAPPLE CV. PÉROLA: 1. PRODUCTION AND FRUIT QUALITY

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    VALMIR PEREIRA DE LIMA

    2001-12-01

    Full Text Available O abacaxizeiro Pérola tem como uma das suas principais características a geração de elevado número de mudas tipo filhote, formando um cacho na parte superior do pedúnculo. Os filhotes desenvolvem-se no mesmo período da formação do fruto. Em plantio comercial na região do litoral Norte da Bahia, foi desenvolvido este estudo com o objetivo de avaliar o efeito do desbaste de mudas tipo filhote sobre aspectos vegetativos e produtivos do abacaxi cv. Pérola, cultivado sob condições de sequeiro. Nesta primeira parte, serão apresentados os efeitos sobre a produção e a qualidade do fruto. Em delineamento em blocos completos ao acaso, com sete repetições, foram estudados seis tratamentos, sendo a testemunha, sem desbaste, e os seguintes cinco níveis de desbaste de mudas tipo filhote: 1 - todas as mudas desbastadas; 2 -mantidas duas mudas do lado do sol poente; 3 - mantidas quatro mudas, com desbaste, de cima para baixo; 4 - mantidas quatro mudas, com desbaste, de baixo para cima; 5 - mantidas seis mudas. Por meio da análise de variância e teste de comparação entre as médias, foram avaliados aspectos da qualidade química e física dos frutos, tais como sólidos solúveis totais (SST, acidez total titulável (ATT, relação SST/ATT, peso do fruto com coroa, comprimento do fruto, peso e comprimento da coroa, produtividade, número e o peso médio dos frutos. O desbaste não determinou diferenças estatísticas para a produtividade da cultura e o peso do fruto, observando-se, no entanto, tendência consistente para a elevação dos valores deste último. O desbaste de mudas não afetou a qualidade do fruto, que apresentou características químicas, físicas e físico-químicas dentro dos padrões da cultivar Pérola.One of the main characteristics of the pineapple cv. Pérola is the generation of numerous plantlets called slips located at the upper part of the peduncle. The slips develop at the same period of fruit formation. In a

  6. Efeito do hipoclorito de sódio sobre a microbiota de abacaxi 'Pérola' minimamente processado Effect of sodium hypochlorite on the microflora of fresh-cut 'Pérola' pineapple

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    Lucimara Rogéria Antoniolli

    2005-04-01

    Full Text Available Procurou-se determinar a menor concentração eficiente de hipoclorito de sódio (NaOCl para desinfecção da casca, bem como avaliar a necessidade de utilização do mesmo agente sanitizante no banho de imersão da polpa do abacaxi 'Pérola' minimamente processado. Frutos previamente lavados foram desinfectados com NaOCl 100; 150 ou 200mg.L-1, durante 2 minutos. Após aproximadamente 24 horas de armazenamento refrigerado, os frutos foram descascados e fatiados mecanicamente. As fatias foram imersas em solução de NaOCl 20mg.L-1 ou em água (controle, durante 30 segundos. Após período de repouso, para drenagem do excesso de líquido, foram acondicionadas em embalagens de polietileno tereftalato e mantidas à temperatura de 4 ± 1°C, durante 16 dias. As análises microbiológicas, realizadas em intervalos de três dias, foram: coliformes a 35ºC, coliformes a 45ºC, bactérias aeróbias mesófilas e de bolores, e leveduras. Não foram detectados coliformes totais e fecais em nenhum dos tratamentos, durante 16 dias de armazenamento refrigerado. A desinfecção da casca com NaOCl 200mg.L-1, associada à sanitização da polpa do abacaxi com NaOCl 20mg.L-1, proporcionou menores populações de microrganismos aeróbios mesófilos e de bolores, e leveduras, tornando tais operações imprescindíveis na obtenção de produtos minimamente processados que ofereçam garantia de sanidade ao consumidor.The purpose of this research was to determine the smallest effective concentration of sodium hypochlorite (NaOCl for intact fruit disinfection as well as to evaluate the use of the same disinfectant product for sanitization of the fresh-cut 'Pérola' pineapple. Fruits were washed and disinfected with chlorine solutions (100, 150 or 200mg.L-1 for 2 minutes. After approximately 24 hours of cold storage, fruits were mechanically peeled and sliced. Slices were dipped in 20mg.L-1 of chlorine solution or pure water (control, for 30 seconds. After that, the

  7. Conservation of minimally processed pineapple using calcium chloride, edible coating and gamma radiation;Conservacao de abacaxi minimamente processado utilizando como coadjuvantes cloreto de calcio, pelicula comestivel e radiacao gama

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    Pilon, Lucimeire

    2007-07-01

    The aim of this study was to obtain a convenience type pineapple subjected to fresh-cut process and calcium chloride, wheat gluten and alginate-base edible coating and irradiation treatments. The fruits were washed, sanitized with Sumaveg (Sodium Dichloro-s-Triazinetrione) in a 200 mg L-1 chlorine-free solution at 7 deg C for 15 minutes, and then manually peeled. The peeled fruits were sliced into 1 cm thick slices, rinsed in 20 mg L-1 chlorine-free solution for 3 minutes and drained for 3 minutes. In the first experiment, the samples were treated with: 1% calcium chloride + vital wheat gluten solution; 1% calcium chloride + 1% alginate solution; and control. In the second experiment, the samples were treated with: 1% calcium chloride + vital wheat gluten solution + 2.3 kGy; 1% calcium chloride + 2.3kGy; irradiation with 2.3kGy; and control. The packing consisted of rigid polyethylene terephthalate (PET) trays with around 250 g of fruit. The irradiation was performed in a Cobalt-60 multipurpose irradiator with 92 kCi activity and dose value of 2.3 kGy h-1. The samples were stored at 5 +- 1 deg C and evaluated every other day for 12 days. In the first experiment pH and titratable acidity values showed slight variations but were similar between the treatments. There was a decrease in ascorbic acid values in all treatments. Browning was noticed in all treatments over the storage period. Although the values between the treatments were similar, the pineapple treated with calcium chloride + gluten showed firmer texture, less liquid loss, and lower values of polyphenoloxidase and peroxidase activities and CO{sub 2} and ethylene production. Mesophiles and mold and yeast counts were also reduced. No Salmonella and E. coli were detected. Total coliform counts were low in all the treatments and appeared in just a few isolated samples during the storage period. Sensory analyses showed that the samples treated with calcium chloride + gluten had the lower scores for texture

  8. Caracterización de la maduración del fruto de pina nativa (Ananas comosus L. Merrill CV. India Charaeterization of the ripening of pineapple (Ananas comosus L. Merrill ev. India fruit

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    Morales Madelaide

    2001-12-01

    Full Text Available El presente estudio tuvo como propósito caracterizar física, química y fisiológicamente el desarrollo de la maduración del fruto de piña nativa cv. India, conservado en fresco a 20°C. Durante el periodo de maduración del fruto, se encontró que la intensidad respiratoria no tuvo ningún incremento, lo que permitió clasificar al fruto como no climatérico. Este comportamiento estuvo acompañado por perdida de peso, disminución de la consistencia del mesocarpio del fruto, aumento en ácidos orgánicos, acidez, azúcares y disminución en los sólidos solubles y pH. El análisis de calidad mostró el detrimento de las características organolépticas que hacen apetecible el fruto, como son apariencia, sabor, color y aroma.This study was conducted to characterize physical, chemical
    and physiologically the development of maturation of fruit
    native pineapple cv. India, kept fresh at 20°C. During the fruit
    rnaturation, the respiratory intensity did not have any incrcase,
    which allowed to classify the fruit as non climacteric. This
    behavior was accompanied by loss of weight, decrease of
    consistency of edible fruit portion, increase in organic acids,
    acidity and sugars and decrease in soluble solids and pH.
    The quality analysis showed loss of organoleptic
    characteristics that make desirable the fruit, like appearance,
    flavor and color.

  9. WILD PINEAPPLE (ANANAS BRACTEATUS (LINDL., VAR. ALBUS HARVESTED IN FOREST PATCHES IN RURAL AREA OF VIÇOSA, MINAS GERIAS, BRAZIL: EXCELLENT SOURCE OF MINERALS AND GOOD SOURCE OF PROTEINS AND VITAMIN C

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    GALDINO XAVIER DE PAULA FILHO

    2016-01-01

    Full Text Available ABSTRACT In the rural region of the municipality of Viçosa, Minas Gerais, forest patches are encountered and they present a great diversity of wild and edible fruit, where wild pineapple (Ananas bracteatus (Lindl., var. albus is one of those of greatest occurrence. Given that, little is known about the nutritional characteristics of this fruit, the present study aimed to investigate the physical and physic-chemical characteristics, chemical composition (titratable acidity, total soluble solids, pH, moisture content, ash, protein, lipids and dietary fiber, carotenoids (a-carotene, ß-carotene, ß-cryptoxanthin and lycopene, vitamin C (AA and DHA by HPLC-DAD, vitamin E (a-, ß-, ?-, d-, tocopherols and tocotrienols by HPLC-fluorescence, and minerals (P, K, Ca, Mg, Cu, Fe, Zn, Mn, Na, Cr, Se and Mo by ICP-AES. Fruits showed a moisture content average of 78.5 g 100g-1, 16.3 ° Brix of soluble solids, titratable acidity equal to 1.71 g 100g-1 of citric acid, 1.66 g 100g-1 of dietary fiber, 1.41 g 100 g-1 of lipids and caloric density of 83.15 kcal 100g-1. We considered it a source of carbohydrates (12.82 g 100g-1, proteins (4.79 g 100g-1 and Zn (0.98 mg 100g-1; a good source of vitamin C (18.70 mg 100g-1; and an excellent source of Cu (0.48 mg 100g-1, Fe (2.05 mg 100g-1, Mn (8.87 mg 100g-1 and Mo (0.15 mg 100g-1.

  10. Uréia como fonte alternativa de nitrogênio na micropropagação de abacaxizeiro cv. Pérola = Micropropagation of pineapple cv. Pérola with urea as nitrogen source

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    Maria Aparecida Moreira

    2007-12-01

    Full Text Available Objetivou-se estudar a viabilidade da substituição parcial ou total do nitrato de amônio por uréia como fonte de nitrogênio no meio de cultura para o cultivo in vitro do abacaxizeiro cv. Pérola. Plantas oriundas das gemas da coroa do fruto com massa fresca em torno de 80 mg foram inoculadas em meio MS (líquido e sólido substituindo-se 100, 80, 60, 40, 20 e 0% do nitrato de amônio por uréia. O delineamento experimental utilizado foi o inteiramente casualizado, com quatro repetições e três plantas/repetição. Após 60 dias,observou-se a possibilidade da substituição parcial de 40% de nitrato de amônio por uréia para as variáveis analisadas e melhor desenvolvimento das plantas em meio de cultura sólido.The partial or total substitution viability of the ammonium nitrate for urea, as source of nitrogen in the culture medium for the in vitro culture of pineapple cv. Pérola was studied. Plants originating from fruit crown buds with fresh weight matter around 80 mg was inoculated on MS medium (liquid and solid with substitution of 100, 80, 60, 40, 20 and 0% of the ammonium nitrate for urea. These treatments were arranged in a completely randomized design, with four replications and three plants/replications. After 60 days, thepossibility of 40% ammonium nitrate for urea partial substitution was observed for the analyzed variables. In addition, better plant development in solid culture medium was observed as well.

  11. Sistema de recomendação de fertilizantes e corretivos para a cultura do abacaxi - fertcalc-abacaxi Fertilizer and lime recommendation system for pineapple - fertcalc-abacaxi

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    Alexandre Paiva da Silva

    2009-10-01

    pineapple plants is higher than for other crops and IT depends on cultivar, fruit weight, production destination and planting density and cultivation system. However, the variation in these factors is not always taken into account in fertilization tables. The purpose of this study was to propose a fertilizer and lime recommendation system for pineapple designated FERTCALC-Abacaxi, based on systematization and modeling of literature data and the current knowledge concerning fertilization and mineral nutrition of the crop. FERTCALC-Abacaxi is based on the nutritional balance method and is divided into two subsystems: the requirement subsystem that contemplates the plant demand, considering the efficiency of recovery of the applied nutrients and a dose to meet the requirement "sustainability"; and the supply subsystem that comprises the nutrient supply by liming, soil, organic matter, and by organic residues. Simulations with FERTCALC-Abacaxi showed that the System recommends higher doses for the Pérola cv., than for Smooth Cayenne, for denser planting systems and to obtain heavier fruits; and differently from the tables, it varied continuously with the expected productivity and soil nutrient contents, being higher in the first than in the second growing cycle. In view of the higher number of factors taken into consideration that reflect the response of the crop to fertilization, for example the expected yield, cultivar, fruit weight, planting density and system, besides taking into account the contribution of organic residues from the previous cycle, the FERTCALC- Abacaxi represents a more versatile alternative for fertilizer and lime recommendation for the pineapple crop. However, improvements in future versions of the System as well as its validation under practical conditions are necessary.

  12. Morpho-physiological changes in pineapple plantlets [Ananas comosus (L. merr.] during acclimatization Mudanças morfofisiológicas em plântulas de abacaxi [Ananas comosus (L. Merr.] durante a aclimatização

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    Ariel Villalobo

    2012-12-01

    Full Text Available Pineapple producing countries lack good quality propagation material to expand cultivars into new areas. Previously, research protocols may increase the offer of high quality plantlets and speed the introduction of new pineapple cultivars. The present work is to evaluate the morpho-physiological changes in plantlets of pineapple [Ananas comosus (L. Merr. 'MD-2'] during the acclimatization phase. Plantlets were acclimatized under 80% relative humidity, 25.5 °C temperature and photosynthetic photon flux of 400-500 µmol m-2 s-1 as average for 45 d under natural photoperiods. All measurements (plant length, number of leaves and roots, fresh weight, width and length of leaf 'D', net photosynthesis and total transpiration rate were carried out at the end of in vitro rooting phase coincident with 0 d of acclimatization and at 15, 30 and 45 d thereafter. Photosynthetic activity of in vitro plantlets did not increase during the first 30 d of the acclimatization phase. After 30 d, photosynthetic activity ranged from 5.72 to 9.36 µmol CO2 m-2 s-1 while total transpiration ranged from 6.0 to 1.42 mmol H2O m-2 s-1. During the first 30 days there were no significant differences in number of leaves, length or width of the longest ('D' leaf (cm or plant length (cm. However, after 45 days plant fresh weight (g, length and width of the 'D' leaf (cm and root number all increased significantly, while transpiration (mmol H2O m-2 s-1 declined. There were small but significant decreases in chlorophyll a and b (µg g-1 mf.. Increased photosynthetic activity after 30 d shows that the increase in light intensity and the reduction of relative humidity during acclimatization did not constitute inhibitory factors.Os países produtores do abacaxi precisam de mudas de boa qualidade para a introdução de novas culturas nas áreas de cultivo. Os protocolos de pesquisa podem aumentar a oferta de mudas de alta qualidade e acelerar a introdução de novas culturas do abacaxi

  13. ALTAS DENSIDADES DE PLANTIO NA CULTURA DO ABACAXI CV. SMOOTH CAYENNE, SOB CONDIÇÕES DE SEQUEIRO HIGH PLANTING DENSITY FOR THE SMOOTH CAYENNE PINEAPPLE CROP, GROWN UNDER RAINFED CONDITIONS

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    LUCIANA LIMA DE ALMEIDA SANTANA

    2001-08-01

    Full Text Available No Brasil têm-se usado densidades de plantio relativamente baixas para a obtenção de frutos grandes, com reflexos negativos na produtividade da cultura do abacaxi. No entanto, frutos cada vez menores têm sido comercializados no mercado internacional, o que poderá também ocorrer no mercado nacional. Este trabalho teve o objetivo de avaliar o efeito de altas densidades em sistemas de plantio em filas duplas sobre a produção quantitativa e qualitativa de abacaxi cv. Smooth Cayenne, sob condições de sequeiro. Densidades de plantio, variando de 51.280 plantas/ha a 100.000 plantas/ha, foram estudadas em delineamento experimental de blocos casualizados, com cinco repetições e doze tratamentos, distribuídos em esquema fatorial 4 x 3, correspondendo a quatro combinações de espaçamentos entre linhas duplas e entre linhas simples na fila dupla (90cm x 40 cm, 90 cm x 30 cm, 80 cm x 30 cm , 70 cm x 30 cm e três espaçamentos entre plantas nas linhas de plantio (30 cm, 25 cm, 20 cm. A análise de variância determinou diferença estatística apenas para a produtividade, em função do espaçamento entre plantas na linha de plantio, sendo mais elevada para o espaçamento de 20 cm. O peso do fruto, as suas dimensões e a sua qualidade (açucares, acidez, teor de suco, relação açúcares/acidez não foram significativamente influenciados pelas densidades de plantio estudadas, mantendo-se dentro dos padrões da cultivar. Para cada aumento de 10.000 plantas por hectare, a produtividade cresceu em 8,27 t/ha e o peso médio do fruto caiu 102 g. Nas condições ambientais dos Tabuleiros Costeiros do Norte da Bahia, a cultura do abacaxi cv. Smooth Cayenne apresenta potencial para uso em altas densidades de plantio, mesmo em cultivo de sequeiro, podendo-se atingir produtividade acima de 80t/ha e peso médio do fruto superior a 1,0 kg.In Brazil, relatively low planting densities have been used to obtain large fruits, with negative effects on pineapple

  14. Pineapple yield and fruit quality effected by NPK fertilization in a tropical soil Produção e qualidade de frutos de abacaxizeiro em resposta à adubação com NPK

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    Ademar Spironello

    2004-04-01

    Full Text Available There is a lack of information about fertilization of pineapple grown in the State of São Paulo, Brazil. So a field experiment with pineapple 'Smooth Cayenne' was carried out to study the effects of NPK rates on yield and fruit quality. The trial was located on an Alfisol in the central part of the State of São Paulo (Agudos county. The experimental design was an incomplete NPK factorial, with 32 treatments set up in two blocks. The P was applied only at planting, at the rates of 0; 80; 160 and 320 kg/ha of P2 0(5, as superphosphate. The N and K2O rates were 0; 175; 350, and 700 kg/ha, applied as urea and potassium chloride, respectively, divided in four applications during the growth period. Response functions were adjusted to yield or to fruit characteristics in order to estimate the nutrient rates required to reach maximum values. The results showed quadratic effects of N and K on yield and a maximum of 72 t/ha of fresh fruit was attained with rates of 498 and 394 kg/ha, respectively of N and K2O. In order to reach the maximum fruit size, and to improve the percentage of first class fruit (mass greater than 2.6 kg, were necessary rates of N and K respectively 11 and 43 % higher than those for maximum yield. No effect of P rates was observed on pineapple plant growth, despite the low availability of this nutrient in the soil. The effect of N rates was negative on total soluble solids and total acidity while the opposite occurred with K, which increased also the content of vitamin C. High yield and fruit size were closely related to N and K concentrations in the leaves.Há falta de informações sobre adubação de abacaxizeiro em São Paulo. Assim, um experimento de campo com abacaxizeiro 'Smooth Cayenne' foi desenvolvido com o objetivo de estudar os efeitos de doses de NPK na produção e qualidade de frutos em Agudos, região central do Estado, num Argissolo Vermelho-Amarelo. Empregou-se um fatorial incompleto, com 32 tratamentos distribu

  15. Seleção de bactérias promotoras de crescimento no abacaxizeiro cultivar Vitória durante a aclimatização Selection of growth-promoting bacteria for pineapple 'Vitória' during acclimatization

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    Lílian Estrela Borges Baldotto

    2010-04-01

    Full Text Available A propagação in vitro do abacaxizeiro (Ananas comosus L. Merril resulta na produção de uma grande quantidade de mudas sadias e homogêneas. Apesar dessas vantagens, a necessidade de um longo período de aclimatização onera essa prática agrícola. A aceleração do crescimento das plantas pela inoculação de bactérias diazotróficas endofíticas e epifíticas pode ser útil para diminuir esse período. O objetivo deste trabalho foi avaliar o potencial de 20 estirpes de bactérias diazotróficas em sintetizar indol, solubilizar fosfato de Ca e óxido de Zn e atuar antagonicamente ao fungo fitopatogênico Fusarium subglutinans f. sp. ananas, bem como, posteriormente, avaliar o desempenho do abacaxizeiro 'Vitória' propagado por cultura de tecidos em resposta à inoculação bacteriana durante o período de aclimatização em casa de vegetação. Foram medidas as características de crescimento da parte aérea e do sistema radicular e o conteúdo de nutrientes de folhas do abacaxizeiro. Os resultados mostraram diferenças na capacidade das bactérias de sintetizar indol, solubilizar fosfato de Ca e óxido de Zn e atuar antagonicamente ao Fusarium. Foram também constatadas diferenças na capacidade das bactérias em promover o crescimento da parte aérea e do sistema radicular e o acúmulo de N, P, K, Ca e Mg em folhas do abacaxizeiro. A inoculação das bactérias diazotróficas selecionadas pode promover o crescimento das mudas durante o período de aclimatização, melhorando a adaptação do abacaxizeiro ao ambiente ex vitroPineapple (Ananas comosus L. Merrill in vitro propagation results in the production of a large quantity of healthy and homogeneous plantlets. Despite these advantages, the long time required for acclimatization makes this agricultural practice too costly. Growth acceleration of plantlets by inoculation with endophytic and epiphytic diazotrophic bacteria may be useful to reduce this period. The objectives of this

  16. Avaliação por RAPD de plantas de abacaxizeiro cultivar Smooth Cayenne derivadas do seccionamento do talo e cultura de tecidos Evaluation of pineapple plants cultivar Smooth Cayenne from peduncle division and tissue culture by RAPD

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    MARIA VITÓRIA CECCHETTI GOTTARDI

    2002-04-01

    Full Text Available Foram coletadas, em área comercial da fazenda Córrego dos Bois, município de Canápolis -- MG, plantas de abacaxizeiro cultivar Smooth Cayenne, para serem avaliadas quanto à propagação pelo método do seccionamento do talo e cultura de tecidos, bem como análise por RAPD das mudas decorrentes destes dois processos de propagação. A propagação pelo seccionamento do talo foi eficiente na produção de mudas, tanto em quantidade como em qualidade, em um curto espaço de tempo, além de apresentar a mesma característica genotípica (análise por RAPD das plantas-matrizes de origem. Já no processo de produção de mudas por cultura de tecidos, não foi obtida uma quantidade suficiente de mudas que comprovasse a utilização de uma metodologia mais sofisticada. Além da perda por contaminação em laboratório de 70% do material em estudo, foi necessária a utilização de um longo período, aproximadamente 18 meses, para a obtenção das mudas. Na análise por RAPD das plantas decorrentes deste processo de propagação, foram observados padrões de bandas diferentes em algumas amostras, as quais podem estar relacionadas com uma possível variação somaclonal.Plants of pineapple Smooth Cayenne cultivar were collected from a commercial area, Córrego dos Bois farm, in Canápolis, state of Minas Gerais, to evaluate two different processes of propagation, peduncle division and tissue culture. These same plants were characterized by RAPD analysis. According to the two propagation method, the peduncle division was efficient in seedlings propagation, in quantity as well as in quality, at a short period of time, resulting the same phenotype and genetic characteristics (RAPD analysis from the matrix descent plants. In the tissue culture method, the quantity of seedlings obtained were not good enough that could prove the use of a sophisticated methodology. Besides loosing 70% of the material, it was necessary to expend a long period of time, at

  17. Efeito de substratos na aclimatização de mudas micropropagadas de abacaxizeiro cv. Pérola Acclimatization of micropropagated pineapple plants cv. "Pérola": substrata effect

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    Maria Aparecida Moreira

    2006-10-01

    Full Text Available Objetivou-se com este trabalho verificar o efeito da matéria orgânica como componente de substratos para mudas micropropagadas de abacaxizeiro cv. Pérola em fase de aclimatização. Mudas micropropagadas de abacaxizeiro cv. Pérola, selecionadas de acordo com o peso (aproximadamente 2 g, foram plantadas em bandejas de isopor de 72 células de 120 cm³ contendo proporções de substratos com terra, esterco bovino, Plantmax e matéria orgânica. O delineamento estatístico utilizado foi o inteiramente casualizado com 4 repetições e 3 plantas por parcela. As avaliações foram feitas 90 dias após o plantio, quando se avaliou: altura da planta, comprimento de raiz, peso de matéria fresca de raiz e parte aérea, peso de matéria seca de raiz e parte aérea e número de folhas. Com os resultados obtidos, conclui-se que a matéria orgânica tem efeito significativo no desenvolvimento das mudas. Com o substrato contendo terra, esterco e Plantmax foi obtido o melhor desenvolvimento das raízes e parte aérea.The objective of this work was to evaluate the effect of the organic matter as component of substrata for micropropagated plants of pineapple cv. Pérola in the acclimatization phase. Micropropagated plants were selected according to the weight (approximately 2g and planted in trays with 72 cels of 120 cm³, containing substrata made of soil, bovine manure, Plantmax and organic matter at different proportions. At 90 days after planting, the following parameters were evaluated: height of the plant, root length, weight of fresh matter of root and aerial part, dry matter weight of root and aerial part and number of leaves. The obtained results allowed to conclude that the organic matter has significant effect on the development of the seedlings. The best results for the development of the roots and aerial part were obtained with substratum containing soil, manure and Plantmax.

  18. AVALIAÇÃO DE PLANTAS MATRIZES DE ABACAXIZEIRO CULTIVAR SMOOTH CAYENNE UTILIZANDO MARCADORES RAPD E PADRÕES ISOENZIMÁTICOS EVALUATION OF MATRIX PLANTS OF PINEAPPLE SMOOTH CAYENNE CULTIVAR BY RAPD AND ISOZYMES ANALYSIS

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    MARIA VITÓRIA CECCHETTI GOTTARDI

    2001-12-01

    Full Text Available Foram coletadas, em área comercial da fazenda Córrego dos Bois, município de Canápolis -- MG, 20 plantas matrizes de abacaxizeiro cultivar Smooth Cayenne, para avaliação de similaridade e padrões genotípicos através de marcadores moleculares RAPD e padrões isoenzimáticos. As plantas matrizes foram selecionadas mediante as seguintes características: planta sadia, frutos cilíndricos, ausência de fasciação, pedúnculo curto, ausência de espinhos nas folhas, "olho" do fruto chato e peso dos frutos entre 1,6 a 2,2kg. Para análise de marcadores RAPD, foram testados 100 "primers", dos quais, 43 foram eficientes na amplificação das amostras, onde foram observados padrões de bandas diferentes entre as plantas matrizes utilizadas, indicando a existência de variabilidade genética. Nos padrões isoenzimáticos, dos 15 sistemas utilizados para revelação das amostras, 8 apresentaram atividade enzimática, sendo 5 deles com baixa resolução; entretanto, estes sistemas não foram eficientes em diferenciar as amostras devido à ausência de polimorfismoTwenty matrix plants of pineapple cultivar Smooth Cayenne were collected from commercial area in the farm Córrego dos Bois, Canápolis city - MG, to evaluate similarity and genotypes patterns by both molecular markers, RAPD and isozymes analysis. Collected matrix plants presented the following characteristics: healthy plants, cylindrical fruits, no fasciation, short peduncle, spineless leaves, boring bubilles and fruit weight between 1,6 to 2,2kg. One hundred primers were tested to analyze RAPD patterns, within them, 43 were efficient to amplify the samples. Distinct patterns were observed among the matrix plants indicating that there is genetic variability. In relation to isozyme profiles, 15 isoenzyme systems were tested but only 8 revealed enzymatic activity, where 5 of them presented low resolution. In spite of this, the 8 systems weren't efficient to differentiate the samples not

  19. Aplicação de revestimento comestível em abacaxis processados por métodos combinados: isoterma de sorção e cinética de desidratação osmótica Application of edible coatings in processed pineapples products by hurdle technology: desorption isotherms and kinetics of the osmotic dehydration

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    Renata Paula Herrera Brandelero

    2005-06-01

    Full Text Available A desidratação osmótica é uma etapa essencial na elaboração de produtos de fruta através da Tecnologia dos Métodos Combinados ou de Obstáculos ("Hurdle Technology", pois reduz a atividade de água para níveis que, combinando um ou mais obstáculos, aumentam a estabilidade do produto. Neste processo há uma perda de água da fruta para a solução e incorporação de sólidos solúveis pelo produto. Este último fluxo é considerado uma desvantagem do processo pois pode alterar o sabor do produto. Neste trabalho estudou-se a ação de revestimentos comestíveis a base de alginato e gelatina aplicados em abacaxis, previamente à desidratação osmótica, como barreira à incorporação de sólidos solúveis. Os abacaxis com e sem revestimento (controle foram desidratados em solução de sacarose sob condições isotérmicas. Foram determinadas as isotermas de dessorção de abacaxis revestidos com gelatina, alginato e sem revestimento e os parâmetros cinéticos do processo de desidratação osmótica. Abacaxis revestidos com alginato apresentaram menor velocidade de ganho de sólidos, sem alterar a velocidade de perda de água, quando comparado ao controle.The osmotic dehydration is an essential stage to manufacture fruit products by Hurdle Technology because it reduces the water activity to levels that, combined with one or more obstacles, increase the stability of the product. This process causes a loss of water from the fruit to solution and soluble solid incorporation by the product. This last flow is considered a disadvantage of the process because it can modify the product flavor. In this work, the action of alginate and gelatin edible coatings as a barrier to soluble solid incorporation during osmotic dehydration was studied. Pineapples with and without coating (control were dehydrated in sucrose solution under isothermal conditions. The desorption isotherms of pineapples coated with gelatin, sodium alginate and without coating

  20. Viabilidade econômica do cultivo de abacaxi no arenito Caiuá, região noroeste do Estado do Paraná = Economic feasibility of pineapple culture in the Caiuá sandstone area, northwestern Paraná State

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    Aguinaldo José Freitas Leal

    2009-04-01

    Full Text Available A área da região noroeste do Estado do Paraná, sobre o arenito Caiuá, carece de opções de exploração agrícola economicamente viáveis. Assim, o abacaxi apresenta-se como opção de cultivo, entretanto, necessitando de maiores estudos referentes à sua exploraçãonesta região. O presente trabalho teve por objetivo avaliar a viabilidade socioeconômica do cultivo desta frutífera na região de Umuarama, Estado do Paraná em relação às principais regiões fornecedoras de abacaxi para o Estado. Para tanto, visitaram-se três propriedades nos municípios de Umuarama, Cafezal do Sul e Cruzeiro do Oeste, objetivando levantar os coeficientes técnicos desta atividade e seus indicadores de lucratividade. A região analisada apresenta boa capacidade competitiva em relação às regiões tradicionalmente produtoras (Frutal, Estado de Minas Gerais e Guaraçaí, Estado de São Paulo, principalmente pela proximidade do mercado consumidor. O abacaxi é uma excelente opção de cultivo no contexto da agricultura familiar.The northwestern region of the state of Paraná, in the Caiuásandstone area, lacks options for economically feasible agricultural exploration. Thus, pineapple appears as a growth option, needing further studies regarding its exploration in that region. Thepresent review aimed to evaluate the feasibility of this culture and analyze the competitiveness of this region of Umuarama, Paraná State, compared to the state’s main pineapple supplying regions. For this purpose, three producers in Umuarama, Cafezal do Sul and Cruzeiro do Oeste were visited, in order to survey the technical coefficient of this culture and its profit indicators. The analyzed region presents a good and competitive capacity compared to other traditional fruitproducers, mainly due to the proximity of consumer markets. Pineapple is an excellent choice of culture in the familiar agricultural context.

  1. Desbaste de mudas tipo filhote do abacaxi cv. Pérola -- 2: análises de crescimento e de correlações Slips thinning from the pineapple cv. Pérola - 2: growth and correlation analyses

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    VALMIR PEREIRA DE LIMA

    2002-04-01

    Full Text Available O abacaxi cv. Pérola, variedade mais plantada no Brasil, forma um número excessivo de mudas tipo filhote durante o mesmo período de desenvolvimento do fruto. Em plantio comercial da região do litoral Norte da Bahia, foi desenvolvido estudo com o objetivo de avaliar o efeito do desbaste de mudas tipo filhote sobre aspectos vegetativos e produtivos da cultura, desenvolvida sob condições de sequeiro. Nesta segunda parte, são apresentados os efeitos sobre o acúmulo de matérias fresca e seca nos diversos órgãos da planta e analisadas as correlações entre caracteres vegetativos e do fruto. Em delineamento em blocos completos ao acaso, em parcelas subdivididas, com sete repetições, foram estudados seis tratamentos, sendo a testemunha (sem desbaste e cinco níveis de intensidade de desbaste de mudas tipo filhote, e três épocas de avaliação. Por meio da análise de variância, teste de comparação entre as médias e coeficientes de correlação, foram avaliados caracteres de crescimento vegetativo em pesos fresco e seco (planta inteira, raiz, caule, pedúnculo, folhas e mudas aos 90; 120 e 150 dias após o tratamento de indução floral, e a sua correlação com caracteres do fruto. O desbaste favoreceu o acúmulo de matéria seca nas mudas tipo filhote remanescentes e na coroa do fruto, que é outro tipo de muda. A eliminação de todos os filhotes determinou a alteração da correlação entre o peso do fruto e o peso da coroa de negativa para positiva. A distribuição de matéria seca entre os órgãos mostrou partição em favor do fruto, com redução do peso seco de caule, folhas e pedúnculo na fase de maturação do fruto, a partir de 120 dias após o tratamento de indução floral.Pineapple plants of the cv. Pérola, the most cultivated in Brazil, form an excessive number of slips during the same period of the fruit development. In a commercial plantation of the North Coast region of Bahia State, Brazil, was carried out a

  2. Produtividade e qualidade de abacaxizeiro cv. Smooth Cayenne, cultivado comaplicação de doses e parcelamentos do nitrogênio, em Guaraçaí-SP Productivity and quality of pineapple cv. Smooth Cayenne, cultivated with nitrogen doses and splitting application in Guaraçaí-SP

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    Leandro Spegiorin Marques

    2011-09-01

    Full Text Available A adubação é um dos fatores que influem na produção e qualidade dos frutos; portanto, a necessidade de se estudar o efeito do nitrogênio aplicado antes e após a indução floral nestas características dos frutos de abacaxi é fundamental. Os objetivos deste trabalho foram avaliar a produtividade, o estado nutricional e os aspectos quantitativos e qualitativos dos frutos de abacaxizeiro 'Smooth Cayenne' submetidos a doses e parcelamentos do nitrogênio, cultivado em Guaraçaí-SP. O delineamento experimental adotado foi o de blocos ao caso, com quatro repetições e dezesseis tratamentos, num esquema fatorial 4x4, sendo quatro doses de N: 7,5; 15; 22,5 e 30 g planta-1 de N, e quatro parcelamentos: toda a dose aplicada antes da indução floral, metade da dose antes da indução floral e a outra metade após a indução, 2/3 da dose antes da indução e 1/3 da dose após a indução e toda a dose após a indução floral. A aplicação total do N após a indução floral, independentemente da dose, resultou em menores teores foliares de N e dimensões da folha "D", apresentando na colheita frutos pequenos, com menor índice de maturação e maior acidez. Para obtenção de frutos de abacaxi cv. Smooth Cayenne, com maior tamanho, massa e maior teor de SST e menor acidez, a adubação nitrogenada pode ser aplicada em dose total, antes da indução floral, ou parcelada 2/3 antes e 1/3 após esta indução. A adubação nitrogenada incrementou os teores foliares de N, entretanto não teve efeito nas dimensões, produtividade e qualidade de frutos de abacaxizeiro cv. Smooth Cayenne.Fertilization is one of the factors that influence the production and fruit quality, thus the need to study the nitrogen effect applied before and after the floral induction in pineapple fruit characteristics is fundamental. The aim of this study was to evaluate the productivity, nutritional status and the quantitative and qualitative aspects of pineapple fruits

  3. Avaliação da influência da temperatura e do tratamento enzimático no comportamento reológico do suco de abacaxi pérola (Ananas Comosus L. merr. Evaluation of influence from temperature and enzymatic treatment in the rheological behavior of pineapple (Ananas Comosus L. merr. juice

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    Adriano Cesar Calandrini Braga

    2013-03-01

    Full Text Available O objetivo deste trabalho foi determinar o comportamento reológico do suco de abacaxi- pérola natural e tratado com enzimas pectinolíticas. As condições de tratamento enzimático foram otimizadas através de um planejamento experimental do tipo fatorial completo 2k, com três repetições do ponto central. Na avaliação do comportamento reológico foram utilizadas duas amostras submetidas a peneiramento (N e D, analisadas em quatro diferentes temperaturas (10; 25; 50 e 65 °C. As análises reológicas foram realizadas utilizando um viscosímetro de cilindros concêntricos Brookfield e os dados experimentais foram ajustados ao modelo de Mizrahi-Berk. A relação entre temperatura e viscosidade aparente foi descrita por uma equação tipo Arrhenius. A otimização da atividade enzimática indicou, através da análise de variância e da metodologia de superfície de resposta, que as variáveis temperatura e tempo de tratamento exerceram efeito estatisticamente significativo (pThe objective this study was determinate the rheological behavior of pineapple juice natural and treated with pectinolytic enzymes. Tests for optimization of enzyme activity used in treatment were realized using a complete factorial planning 2k, with three repetitions of center point and two independent variables (temperature and time of treatment. In evaluation of rheological behavior of pineapple juice, were utilized two samples submitted to sieving (N and D, analyzed in four different temperatures (10, 25, 50 e 65 °C. The rheological analyses were realized using a Brookfield concentric cylinders viscometer and the relationship in the temperature and apparent viscosity was described by an equation type Arrhenius. The experimental data were adjusted by the Mizrahi-Berk model. The optimization tests indicated by ANOVA and response surface methodology that the variables temperature and time of treatment have statistically significant effect (p<0,05 on concentration of

  4. Method by chromatography of gases for the determination of made up of alcoholic fermentation in pineapple fruits (Ananas comosus [L.] Merr); Metodo por cromatografia de gases para la determinacion de compuestos de fermentacion alcoholica en frutos de pina (Ananas comosus [L.] Merr)

    Energy Technology Data Exchange (ETDEWEB)

    Murillo Williams, A

    2001-07-01

    The pineapple (Ananas comosus) it is used in the entire world for the fresh consumption or for processed products (canned, frozen, dehydrated). it is cultivated in a wide range of countries and in extreme latitudes.The factors of quality include: maturity, stability, size uniformity, absence of microbial deterioration, absence of burns for the sun, absence of blows, damage for insects and breaking, crowns, color, longitude and integrity. The factors that determine the longevity of a product can be physiologic or pathological. The physiologic conditions refer to the processes of degradation of the fabrics after the crop, while the pathological ones involve the attack of mushrooms and bacteria. In the case of the pineapple, a physiologic problem exists, called black heart, internal brewing (IB), brown endogenic stain ({sup m}ancha cafe endogena{sup ,} MCE) or chilling injury (CI) that can happen in any part of the world where it is cultivated. This problem has associated to the exhibition from the pineapple to low temperatures, so it is a challenge to manage a fruit like the pineapple that it cannot tolerate low temperatures without problems. In studies made about the physiologic changes that happen during the storage in controlled atmospheres in fruits, it has been observed that the ethanol and the acetaldehyde are volatile compounds associated with metabolic post-crop changes and that they have implication in the quality of the product. (author) [Spanish] La pina (Ananas comosus) se utiliza en todo el mundo para el consumo fresco o para productos procesados (enlatados, congelados, deshidratados). Se cultiva en un amplio rango de paises y en latitudes extremas. Los factores de calidad incluyen: madurez, firmeza, uniformidad de tamano, ausencia de deterioro microbiano, ausencia de quemaduras por el sol, ausencia de golpes, dano por insectos y quebraduras, corona, color, longitud e integridad. Los factores que determinan la longevidad de un producto pueden ser

  5. Growth promotion of pineapple 'vitória' by humic acids and burkholderia spp. during acclimatization Promoção do crescimento do abacaxizeiro 'vitória' por ácidos húmicos e Burkholderia spp. durante a aclimatização

    Directory of Open Access Journals (Sweden)

    Lílian Estrela Borges Baldotto

    2010-10-01

    Full Text Available In vitro propagation of pineapple produces uniform and disease-free plantlets, but requires a long period of acclimatization before transplanting to the field. Quicker adaptation to the ex vitro environment and growth acceleration of pineapple plantlets are prerequisites for the production of a greater amount of vigorous, well-rooted planting material. The combination of humic acids and endophytic bacteria could be a useful technological approach to reduce the critical period of acclimatization. The aim of this study was to evaluate the initial performance of tissue-cultured pineapple variety Vitória in response to application of humic acids isolated from vermicompost and plant growth-promoting bacteria (Burkholderia spp. during greenhouse acclimatization. The basal leaf axils were treated with humic acids while roots were immersed in bacterial medium. Humic acids and bacteria application improved shoot growth (14 and 102 %, respectively, compared with the control; the effect of the combined treatment was most pronounced (147 %. Likewise, humic acids increased root growth by 50 %, bacteria by 81 % and the combined treatment by 105 %. Inoculation was found to significantly increase the accumulation of N (115 %, P (112 % and K (69 % in pineapple leaves. Pineapple growth was influenced by inoculation with Burkholderia spp., and further improved in combination with humic acids, resulting in higher shoot and root biomass as well as nutrient contents (N 132 %, P 131 %, K 80 % than in uninoculated plantlets. The stability and increased consistency of the host plant response to bacterization in the presence of humic substances indicate a promising biotechnological tool to improve growth and adaptation of pineapple plantlets to the ex vitro environment.A propagação in vitro de abacaxizeiro produz mudas uniformes e sadias, mas exige longo período de aclimatização antes da transferência para o campo. A adaptação ao ambiente ex vitro seguida da

  6. Uso da vinhaça no abacaxizeiro em solo de baixo potencial de produção Stillage application for pineapple in soil with low potential of yield

    Directory of Open Access Journals (Sweden)

    Miralda Bueno de Paula

    1999-07-01

    Full Text Available O trabalho foi desenvolvido com o objetivo de avaliar a vinhaça como fonte de K para o abacaxizeiro (Ananas Comosus L., em substituição ao KCl, e seus efeitos sobre as características químicas do solo. O experimento foi instalado em um LV, textura argilosa, utilizando a cv. Smooth Cayenne. Os tratamentos constaram de quatro doses de vinhaça (0 - 100 - 200 - 400 m³/ha, mais um tratamento adicional: 12 g/planta K2O (KCl. O delineamento experimental foi em blocos ao acaso, com três repetições. Verificou-se efeito significativo da vinhaça e do KCl sobre a produção. Com 400 m³/ha de vinhaça e 20,5 g/planta de KCl , os rendimentos tiveram um acréscimo de 70% e 73%, respectivamente, em relação à testemunha. O fornecimento de K elevou a porcentagem de acidez titulável total e sólidos solúveis totais nos frutos, porém não houve diferença significativa entre as fontes. Os teores foliares de K foram aumentados significativamente pela aplicação de vinhaça e de KCl, e os teores de Mg decresceram. A aplicação de vinhaça contribuiu para aumento, no solo, dos teores dos cátions K, Ca, Mg e para a lixiviação de K. Vinhaça e KCl elevaram a níveis adequados, para cultura, a porcentagem de K na soma de bases.An experiment was carried out in order to evaluate the stillage as K source for pineapple (Ananas Comosus L. in comparison with KCl, and the effects on chemical soil characteristics. The experiment was assessed in a Red Yellow Latosol with Smooth Cayenne cultivar. The treatments consisted of four application doses of stillage (0 - 100 - 200 - 400 m³/ha and of an additional treatment (12 g/plant K2O KCl. The design of the trial was in randomized blocks with three replications. Yields statistically responded to stillage and KCl and yields obtained with 400 m³/ha of stillage and 20.5 g of KCl per plant were 70% and 73%, respectivelly, over the control. Addition of K increased the content of tritratable acidity and total

  7. Caracterização física, química, microbiológica e sensorial de geléias light de abacaxi Physical, chemical, microbiological and sensory characterization of light jellies of pineapple

    Directory of Open Access Journals (Sweden)

    Graziele Guimaraes Granada

    2005-12-01

    Full Text Available O trabalho objetivou elaborar geléias com reduzido teor calórico, utilizando como agentes de corpo diferentes combinações dos hidrocolóides xantana, carragena e locusta. Como comparativo utilizou-se uma formulação padrão de geléia de abacaxi (controle, elaborada com sacarose e xarope de glicose na proporção de 4:1 (p/p, e com teor final de sólidos solúveis de 65ºBrix. As quatro formulações light foram preparadas pela substituição de 50% da quantidade de açúcar da formulação controle, sendo que para equiparar a doçura, adicionou-se o edulcorante sucralose. Foram avaliadas as características químicas, físicas, microbiológicas e sensoriais, além do valor calórico total das geléias. Os resultados mostraram que as geléias apresentaram características químicas e físicas semelhantes às descritas na literatura e enquadraram-se na categoria de produtos light, segundo a legislação vigente. Quanto às características microbiológicas, todas as formulações enquadraram-se nos padrões microbiológicos estabelecidos pela legislação brasileira. Em termos sensoriais a formulação com a combinação das gomas carragena:xantana:locusta (1:1:1, p/p/p foi a que apresentou melhores características de geléia.The objective of the work was to elaborate jellies with reduced caloric content, by using as body replacers a combination of the hydrocolloids xanthan, carrageenan and locust. The pineapple jelly was used as control formulation, and it was made with sucrose and glucose in the proportion of 4:1 p/p, and soluble solid content of 65ºBrix. The four light formulations were prepared by substitution of 50% of the amount of sugar of the control formulation and, to compensate the sweetness, the low-calorie sweetener sucralose was used. The chemical, physical, microbiological, sensory and the total caloric content of the jellies were evaluated. The results showed that the jellies presented chemical and physical characteristics

  8. Avaliação física e química de produtos minimamente processados de abacaxi-'Pérola' Physical and chemical study of minimally processed products of 'Pérola' pineapples

    Directory of Open Access Journals (Sweden)

    BIANCA SARZI

    2002-08-01

    Full Text Available O objetivo deste trabalho foi avaliar, física e quimicamente, produtos minimamente processados de abacaxi-'Pérola', "rodelas" e "metades", armazenados sob diferentes temperaturas (3ºC, 6ºC e 9ºC. Frutos selecionados, quanto ao grau de maturação e ausência de danos, foram lavados, desinfeccionados com cloro (200 mg.L-1 e armazenados a 10ºC, por 12 horas, antes do processamento. O produto minimamente processado foi embalado em bandejas de isopor recobertas com filme de PVC esticável ("metades" ou bandejas de tereftalato de polietileno ("rodelas" e armazenado sob refrigeraç ;ão, com avaliação a cada 3 dias, quanto à textura, coloração, pH, e conteúdos de sólidos solúveis totais, acidez total titulável, ácido ascórbico e de açúcares, solúveis e redutores. Durante o armazenamento, o produto tornou-se menos firme, e sua polpa apresentou escurecimento. Os conteúdos de açúcares solúveis e redutores e de sólidos solúveis totais não foram afetados pelo tipo de preparo, temperatura ou embalagem. Os teores de acidez total titulável aumentaram e foram influenciados pela temperatura, sendo que os mantidos a 9ºC apresentaram os maiores teores, havendo, como conseqüência, decréscimo no pH. Os produtos armazenados a 9ºC também apresentaram evolução mais rápida no escurecimento, na redução do teor de ácido ascórbico e menor vida útil (6 dias, enquanto, para os armazenados a 3ºC e 6ºC, este período foi de 9 dias. Os resultados obtidos permitiram concluir que a temperatura de armazenamento foi o fator limitante para a vida útil destes produtos.The aim of the present study was to determine the physical and chemical characteristics of minimally processed products of 'Pérola' pineapples, "roundels" and "halves," stored under different temperatures (3ºC, 6ºC and 9ºC. The fruits, selected according to degree of maturation and absence of injury, were washed, disinfected with chlorine (200 mg.L-1 and stored at 10º

  9. Alterações morfofisiolócias de plantas de abacaxizeiro influenciadas por diferentes substratos durante o processo de aclimatização Morphophysiological changes of pineapple plants influenced by different substrates during the process of acclimatization

    Directory of Open Access Journals (Sweden)

    Francyane Tavares Braga

    2011-10-01

    Full Text Available Objetivou-se determinar um substrato adequado para aclimatização de plantas micropropagadas de abacaxizeiro 'Gomo de Mel' e a sua caracterização anatômica, durante este processo. O enraizamento dos brotos foi realizado em meio MS acrescido de 30 g L-1 de sacarose e 6 g L-1 de ágar. As culturas foram mantidas em sala de crescimento a 25±1º C, 36µmol m-2 s-1 durante 16 horas diárias. Após 60 dias, brotos enraizados foram removidos dos frascos e distribuídos em tubetes, contendo os seguintes tratamentos: 1 A+X+H (areia, xaxim e húmus (1:1:1; 2 substrato comercial plantmax®; 3 vermiculita e 4 combinação 1:1 de plantmax® + vermiculita. As características anatômicas foram avaliadas nas plântulas ainda in vitro e aos 7; 15; 30 e 60 dias de aclimatização. As folhas de transição também foram caracterizadas. O experimento foi instalado em delineamento inteiramente casualizado, com quatro tratamentos e quatro repetições com cinco plantas. Maiores comprimentos da parte aérea, massa fresca e seca da parte aérea e raízes, foram observados com o uso de areia + xaxim + húmus. Para número de folhas, massa fresca de raízes e massa seca de parte aérea, não houve diferença entre os substratos. Quanto às características anatômicas, o substrato vermiculita, no período de 60 dias de aclimatização, promoveu as maiores espessuras dos tecidos que compõe o limbo foliar.This work aimed to determine a right substrate for acclimatization of pineapple 'Gomo de Mel' plantlets and its anatomical characterization during this process. Rooting of the shoots was done in MS medium supplemented with 30 g L-1 sucrose and 6 g L-1 agar. Cultures were maintained in growth room at 25±1º C, 36µmol m-2 s-1 during 16 hours. After 60 days, rooted shoots were removed from the bottles and distributed in tubes containing the following treatments: 1 A+X+H (sand, fern tree fiber and humus (1: 1: 1; 2 commercial substrate Plantmax®; 3 vermiculite and 4

  10. Desenvolvimento vegetativo de abacaxizeiros 'Pérola' e 'Smooth Cayenne' no Estado da Paraíba Vegetative development of Pérola and Smooth Cayenne pineapple cultivars in the state of Paraíba

    Directory of Open Access Journals (Sweden)

    Alessandra Alves Rodrigues

    2010-03-01

    Full Text Available Este trabalho teve por objetivo avaliar o desenvolvimento vegetativo de abacaxizeiros 'Pérola' e 'Smooth Cayenne' nas condições edafoclimáticas de Santa Rita, Estado da Paraíba. Foram realizados dois experimentos, um para cada cultivar, em Espodossolo Ferrocárbico de textura arenosa, entre junho de 2003 e setembro de 2004. O delineamento experimental foi o de blocos casualizados, com cinco tratamentos (avaliações aos 4; 6; 8; 10 e 12 meses após o plantio e cinco repetições. Foram avaliadas as variáveis de altura da planta, produção de matéria fresca e seca de raízes, caule e folhas e de matéria fresca da folha 'D' na época de indução floral. Os resultados mostraram que as plantas da cv. Pérola apresentaram maiores valores de altura (133 e 100 cm, matéria fresca (263 e 255 g e seca de raiz (80 e 60 g, matéria fresca (310 e 200 g e seca de caule (86 e 20 g em relação à cv. Smooth Cayenne. O acúmulo de matéria fresca e seca das folhas intensificou-se a partir dos oito meses após o plantio, sendo maior para a cv. Pérola, e obedeceu à seguinte sequência: D>C>B>A. As plantas das duas cultivares apresentaram valores de matéria fresca da folha 'D' na época de indução floral superiores a 80 g (118 g para a 'Pérola' e 81 g para a 'Smooth Cayenne', demonstrando a possibilidade de antecipação desta prática.This study was carried out to evaluate the vegetative development of both pineapple plants 'Pérola' and 'Smooth Cayenne' under the edaphoclimatic conditions in Santa Rita county, in the state of Paraíba. Two experiments were conducted, being one for each cultivar, during the period from June 2003 to September 2004 and the sandy-textured 'Espodossolo Ferrocárbico' was used. The randomized block experimental design was used, with five treatments (evaluations at 4, 6, 8, 10 and 12 months after planting and five replicates. The following variables were evaluated: plant height, both fresh and dry matter yield of the

  11. 21 CFR 145.180 - Canned pineapple.

    Science.gov (United States)

    2010-04-01

    ... ingredients referred to in paragraph (a)(3)(i) of this section are: (a) Sugar. (b) Invert sugar sirup. (c) Any... surface of the unit: deep fruit eyes, pieces of shell, brown spots, bruised portions, and other...

  12. 21 CFR 146.185 - Pineapple juice.

    Science.gov (United States)

    2010-04-01

    ... reconstituted with water suitable for the purpose of maintaining essential composition and quality factors of... Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION CANNED FRUIT JUICES Requirements for Specific Standardized Canned Fruit Juices and Beverages § 146...

  13. Bitter Sweets: Mapping Pineapples, Hospitality, and Slavery

    Science.gov (United States)

    Dawson, Julia; Mitchell, Jerry T.

    2017-01-01

    The objects that represent our material culture--street signs, mascots, symbols etched into stone or printed on stationery--are silent bearers of history: the past tattooed on the present. The meanings given to those objects may change from one social or culture group to another. One seemingly innocent and overlooked example in this vein is the…

  14. Testing market integration for Kenyan pineapples. | Onyuma ...

    African Journals Online (AJOL)

    The market structure was found to be oligopsonistic in nature with aspects of collusion amongst the urban middlemen and local market traders thus barring further entry by other potential actors. There was little market integration between urban markets and producing markets, and very low or no integration between the ...

  15. EFFECT OF DETARCHMENT TIME OF PINEAPPLE

    African Journals Online (AJOL)

    user

    Journal of Agriculture and Social Research, Vol. 13, No.2 ... crowns were planted in a media consisting of top soil and rice husk on 1:1 volume basis. .... better than those in unshaded fields also, longer storage time depressed number of leaves.

  16. Produção de mudas do tipo rebentão, utilizando coroas de três cultivares de abacaxi inoculadas com fungos micorrízicos Production of seedlings type suckers, using crowns of three cultivars of pineapple inoculated with mycorrhizal fungi

    Directory of Open Access Journals (Sweden)

    Paulo Cesar dos Santos

    2011-09-01

    alternativa para a produção de mudas de abacaxizeiro, sendo mais eficiente na cultivar 'Smooth Cayenne', que produziu 26 mudas em coroas cultivadas até 420 dias após o plantio.One of the main obstacles to the development of pineapple cultivation in Brazil has been the absence of seedlings in both quantity and quality, to spread. Among the alternatives, the obtaining of seedlings is verified starting from the sprouting from crowns of fruits, which are normally discarded by the consumer. Furthermore, the use of arbuscular mycorrhizal fungi (AMF can be an alternative for improving the production of seedlings, since these fungi can shorten the time of seedlings of various fruit. In that sense, the objective of this work was to evaluate the production of seedlings type suckers, using the method of destruction of the apical meristem of the crown of the pineapple plants inoculated with FMAs. For such, we used the randomized complete block design, factorial 3x3, with three cultivars of pineapple ('Smooth Cayenne', 'Pérola' and 'Jupí' and three microbiological treatments (no inoculation, Glomus etunicatum and a mixture of Glomus clarum and Gigaspora margarita, with four replications. The first emissions were recorded at 30, 60 and 90 days after planting for cultivar Smooth, 'Pérola' and 'Jupí', respectively. The pineapple tree 'Smooth Cayenne' produced 80 to 69% seedlings more than the cultivars 'Pérola' and 'Jupí', respectively. In microbiological treatments there was a significant difference for the suckers issued, in which the mixed treatment was the most prevalent issue when compared with treatment with G. etunicatum. Nutritional assessments in the crowns, inoculation with the mixed treatment promoted an increase of 85 and 66% for P, 22 and 13% for N and 6 and 19% for K, compared to treatments G. etunicatum and control respectively. It is concluded that the production of suckers from crown whose principal gem was beheaded is an alternative for the production of pineapple

  17. Boro em abacaxizeiro 'Pérola' no norte fluminense: teores, distribuição e características do fruto Boro in pineapple plants 'pérola' in the north fluminense: contents, distribution and characteristics of the fruit

    Directory of Open Access Journals (Sweden)

    Susana Cristine Siebeneichler

    2008-09-01

    sem sintomas de deficiência, sugerem que tal limite inferior esteja acima do mínimo necessário.The appropriate evaluation of the nutritional state of a pineapple plant and the fulfillment of its nutritional needs are important factors to improve fruit quality and production levels. This study aimed to evaluate the effect of the application of boron on its contents, distribution in the plant and fruit characteristics, in addition to discussing the range of B considered appropriate for pineapple. Boron concentration in different parts of the 'D' leaf was determined along the growth cycle of pineapple plants. The experiment was carried out in São Francisco de Itabapuana-Rio de Janeiro. The experimental design was completely randomized blocks, with 4 replicates and 5 treatments: no B application; monthly foliar sprays of borax, for 6 months before floral induction; monthly foliar sprays of borax, for 6 months after floral induction; monthly foliar sprays of borax, from 6 months before until 6 months after floral induction, and B application, in the axils of the basal leaves of the plant, at 30 days before the floral induction. Each sample consisted of five 'D' leaves, collected at 230, 300, 370, 450 and 520 days after planting. A foliar application of B determined the trend of increasing the total soluble solids content of the pineapple fruit. The weight and size of the fruit were not significantly affected by the treatments. B distribution along the 'D' leaf was not uniform. In general, the largest B concentration was observed in the apical portion of the leaf and the smallest in the non-chlorophyllated portion of the leaf. Borax foliar application increased B concentration in median and apical portions of the leaf but had no effect on basal and non-chlorophyllated portions of the leaf. The lack of symptoms of boron deficiency associated with a B concentration below the minimum limit suggests that the minimum limit is higher than the real limit of pineapple plants.

  18. Adubação foliar com macro e micronutrientes no crescimento de mudas micropropagadas do abacaxizeiro cv. Gold [Ananas comosus (L. Merrill] em diferentes recipientes Foliar fertilization with macro and micronutrients in the growth of plantlets micropropagated of pineapple cv. Gold [Ananas comosus (L. Merrill] in different containers

    Directory of Open Access Journals (Sweden)

    Izaias dos Santos Bregonci

    2008-06-01

    Full Text Available Objetivou-se com este trabalho avaliar o efeito da adubação foliar com macro e micronutrientes no crescimento das mudas micropropagadas do abacaxizeiro cv. Gold [Ananas comosus (L. Merrill], em diferentes recipientes. O experimento foi em esquema fatorial 8x3, adubação foliar em 8 níveis e recipientes em 3 níveis, através de um delineamento inteiramente casualizado com 5 repetições. As mudas foram padronizadas com altura média de 7,12 cm. As adubações foliares foram feitas com uréia, cloreto de potássio, ácido bórico, um formulado comercial com macro e micronutrientes e testemunha (pulverização com água e os recipientes: bandeja de isopor com 200 células; tubete pequeno de 115 cm³; e tubete grande com 300 cm³. O substrato utilizado foi o plantmax hortaliças®. Avaliaram-se as características área foliar, altura de planta e massa seca da parte aérea e da raiz, aos 140 dias do transplantio. Os adubos foliares proporcionaram maior crescimento em área foliar, altura e massa seca da parte aérea às mudas do abacaxizeiro, embora com resultados diferentes. Os adubos foliares não aumentaram a massa seca do sistema radicular. A bandeja de isopor apresentou as menores médias, com todos os adubos foliares para área foliar, altura e massa seca da parte aérea das mudas do abacaxizeiro. O tubete pequeno e o tubete grande apresentaram resultados semelhantes com a maioria dos adubos foliares utilizados.The objective of this work was to evaluate the effect of the foliar fertilization with macro and micronutrients on the growth of the plantlets micropropagated of pineapple cv. Gold [Ananas comosus (L. Merrill] in different containers. The experiment was mounted in factorial arrangement 8x3, with foliar fertilization in 8 levels and containers in 3 levels, through a completely randomized design with five repetitions. The plantlets were standardized with average height of 7,12 cm. The foliar fertilization was used in the urea

  19. Secagem de fatias de abacaxi in natura e pré-desidratadas por imersão-impregnação: cinética e avaliação de modelos Assessment of convective drying models for fresh and osmo-dehydrated pineapple rings

    Directory of Open Access Journals (Sweden)

    Rafael Gomes Dionello

    2009-03-01

    Full Text Available O presente estudo teve como objetivos investigar o efeito da impregnação de sacarose e de açúcar invertido na taxa de secagem por convecção de abacaxi e avaliar a adequação de nove modelos matemáticos na descrição das curvas de secagem. Os testes experimentais foram feitos empregando-se coroas circulares de abacaxi in natura e pré-desidratadas por imersão-impregnação em soluções de sacarose (0,40, 0,44 e 0,47 g.mL-1 e em xarope de açúcar invertido. A pré-desidratação osmótica foi realizada a 40 e 50 ºC, por 2 horas, sob agitação de 60 min-1. A secagem por convecção foi feita em secador do tipo gabinete com bandejas, a 60 ºC e 1,25 m.s-1. O grau de ajuste dos modelos foi avaliado por meio do coeficiente de determinação, da raiz do erro quadrático médio, do erro percentual absoluto médio e por análise de dispersão de resíduos. As maiores taxas de secagem foram obtidas para amostras in natura; naquelas pré-desidratadas osmoticamente, o aumento da concentração da solução ocasionou a diminuição da taxa de secagem. O modelo de Wang e Singh foi o que melhor descreveu a secagem por convecção de coroas circulares de abacaxi.The effect of glucose and invert sugar impregnation on the convective drying rate of pineapple and the suitability of nine mathematical models to describe the drying curves were investigated. Tests were conducted using fresh pineapple rings and samples which were pre-treated by osmotic dehydration in sucrose solutions (0.40, 0.44, and 0.47 g mL-1 and in invert sugar syrup. Osmotic pre-treatments were performed at 40 and 50 ºC for 2 hours at 60 min-1. The convective drying was performed in a tray cabinet dryer using heated ambient air at 60 ºC and 1.25 m.s-1. The adjustment levels of the models were based on the correlation coefficient, the root mean square error, mean absolute percentage error, and residual plot analysis. The highest rates of convective drying were obtained for the fresh

  20. Desempenho do abacaxizeiro 'Vitória' em resposta à aplicação de ácidos húmicos durante a aclimatação Performance of 'Vitória' pineapple in response to humic acid application during acclimatization

    Directory of Open Access Journals (Sweden)

    Lílian Estrela Borges Baldotto

    2009-08-01

    Full Text Available A aclimatação de mudas de abacaxizeiro propagadas por cultura in vitro é um processo lento. A promoção do crescimento radicular pelo uso de ácidos húmicos pode ser útil durante esse processo. O objetivo deste trabalho foi avaliar o desempenho do abacaxizeiro 'Vitória' propagado por cultura de tecidos em resposta à aplicação de diferentes doses de ácidos húmicos isolados de vermicomposto e de torta de filtro durante o período de aclimatação em casa de vegetação. Foram avaliadas as características anatômicas da lâmina foliar, o teor de pigmentos fotossintéticos, o crescimento da parte aérea e do sistema radicular, e o conteúdo de nutrientes foliares. Os resultados mostraram incrementos no crescimento e desenvolvimento da parte aérea e do sistema radicular com a aplicação de ácidos húmicos, bem como acúmulo significativo de N, P, K, Ca e Mg e aumento na relação clorofila a/clorofila b. A promoção do crescimento das mudas durante o período de aclimatação, em resposta à aplicação de ácidos húmicos, pode melhorar a adaptação do abacaxizeiro ao ambiente ex vitro.The acclimatization of pineapple plantlets propagated by plant tissue culture is slow. The intensification of root growth by the use of humic acids may be useful during this process. The aim of this study was to evaluate the greenhouse acclimatization of 'Vitória' pineapple propagated by plant tissue culture in response to different doses of humic acids isolated from vermicompost and filter cake. Leaf anatomy, photosynthetic pigment content, shoot and root growth, and leaf nutrients were evaluated. An increase in growth and development of shoots and root system with the application of humic acids as well as a significant accumulation of N, P, K, Ca, and Mg and increase in the chlorophyll a / chlorophyll b ratio were found. Growth promotion by the application of humic acids to plantlets during the period of acclimatization can improve their